Sample records for lake chad viruses

  1. Lake Chad, Chad, Africa

    NASA Image and Video Library

    1990-04-29

    Africa's Lake Chad where the borders of Chad, Niger, Nigeria and Cameroon merge (13.0N, 14.0E) has been undergoing change for the past 25 to 30 years when it was first noticed that the lake is drying up. Since then, astronauts have been photographing it on a regular basis to record the diminishing lake bed. This lake was once the aproximate size of Lake Erie but is now only about half that size and is still receeding.

  2. Lake Chad, Chad, Africa

    NASA Technical Reports Server (NTRS)

    1992-01-01

    Hydrologic and ecologic changes in the Lake Chad Basin are shown in this Oct 1992 photograph. In space photo documentation, Lake Chad was at its greatest area extent (25,000 sq. km.) during Gemini 9 in June 1966 (see S66-38444). Its reduction during the severe droughts from 1968 to 1974 was first noted during Skylab (1973-1974). After the drought began again in 1982, the lake reached its minimum extent (1,450 sq. km.) in Space Shuttle photographs taken in 1984 and 1985. In this STS-52 photograph, Lake Chad has begun to recover. The area of the open water and interdunal impoundments in the southern basin (the Chari River Basin) is estimated to be 1,900 to 2100 sq. km. Note the green vegetation in the valley of the K'Yobe flow has wetted the northern lake basin for the first time in several years. There is evidence of biomass burning south of the K'Yobe Delta and in the vegetated interdunal areas near the dike in the center of the lake. Also note the dark 'Green Line' of the Sahel (the g

  3. Lake Chad, Chad, Africa

    NASA Technical Reports Server (NTRS)

    1988-01-01

    The fluctuating water levels of Lake Chad, (13.0N, 15.0E) at the intersection of the borders of Chad, Niger and Cameroon in the Sahara Desert, is an index of the drought in Africa. The lake level continues to decrease as indicated by the growing number and extent of emerging islands as previously submerged ancient sand dunes become visible. The water impounded between the dunes is probably because of local rainfall rather than a reversal of desertification.

  4. Lake Chad, Chad as seen from STS-66

    NASA Image and Video Library

    1994-11-14

    This oblique view of Lake Chad was taken by the STS-66 crew in November 1994. This lake lies mainly in the Republic of Chad and partly in Nigeria, Cameroon and Niger. The size of Lake Chad varies seasonally and is actually divided into north and south basins; neither of which is generally more than 25 feet (7.6 meters) deep. In this photograph, all the water appears to be located in the southern basin with the northern and eastern edges of both basins covered with sand dunes which have invaded the area where the water once stood. The prevailing wind direction can be seen from the agriculture burning in both basins to be from the east.

  5. Investigation of soil properties for identifying recharge characteristics in the Lake Chad Basin

    NASA Astrophysics Data System (ADS)

    Banks, M. L.; Ndunguru, G. G.; Adisa, S. J.; Lee, J.; Adegoke, J. O.; Goni, I. B.; Grindley, J.; Mulugeta, V.

    2009-12-01

    Lake Chad was once labeled as one of the largest fresh water lakes in the world, providing water and livelihood to over 20 million people. The lake is shared by six different countries; Chad Nigeria, Niger, Cameroon, Central African Republic, and Sudan. Since the 1970 to date, a significant decrease in the size of the lake has been observed with the use of satellite imagery. This shrinking of the lake has been blamed on global warming, population increase and poor water management by the agriculture industry for farming purpose for both plants and animals. While these can be all valid reasons for the decrease of Lake Chad, we see the need to examine environmental and hydrological evidence around the Lake Chad basin. This study was carried out from upper stream to lower stream leading from Kano to the Damatru region which is one of several water bodies that supply Lake Chad. Over seventy six sites were sampled for soil texture, bulk density and other physical properties to investigate recharge capacity of the basin especially along the stream. Soils were collected using a soil core and properly stored at 4 degrees Celsius. Soils were weighed and put to dry at 105 degrees for twenty four hours. Dry weight was recorded and bulk density was calculated. The wet sieve method was used to determine the particle size analysis. Soils were weighed to 10 grams and hydrogen peroxide added to separate particles. Samples were washed with water and put to dry overnight. Soils were reweighed and sieved to separate as course sand, fine sand and silt and clay. The data revealed that in the upstream, coarse sand continuously decreased while silt and clay continuously increased down toward the lake. At mid stream silt and clay had significantly higher values when compared to coarse sand and fine sand. In the lower stream, bulk density clearly decreased compared to the upper and mid streams. Correlations will be carried out to investigate the particle size analysis and bulk density with

  6. Quaranfil, Johnston Atoll, and Lake Chad viruses are novel members of the family Orthomyxoviridae.

    PubMed

    Presti, Rachel M; Zhao, Guoyan; Beatty, Wandy L; Mihindukulasuriya, Kathie A; da Rosa, Amelia P A Travassos; Popov, Vsevolod L; Tesh, Robert B; Virgin, Herbert W; Wang, David

    2009-11-01

    Arboviral infections are an important cause of emerging infections due to the movements of humans, animals, and hematophagous arthropods. Quaranfil virus (QRFV) is an unclassified arbovirus originally isolated from children with mild febrile illness in Quaranfil, Egypt, in 1953. It has subsequently been isolated in multiple geographic areas from ticks and birds. We used high-throughput sequencing to classify QRFV as a novel orthomyxovirus. The genome of this virus is comprised of multiple RNA segments; five were completely sequenced. Proteins with limited amino acid similarity to conserved domains in polymerase (PA, PB1, and PB2) and hemagglutinin (HA) genes from known orthomyxoviruses were predicted to be present in four of the segments. The fifth sequenced segment shared no detectable similarity to any protein and is of uncertain function. The end-terminal sequences of QRFV are conserved between segments and are different from those of the known orthomyxovirus genera. QRFV is known to cross-react serologically with two other unclassified viruses, Johnston Atoll virus (JAV) and Lake Chad virus (LKCV). The complete open reading frames of PB1 and HA were sequenced for JAV, while a fragment of PB1 of LKCV was identified by mass sequencing. QRFV and JAV PB1 and HA shared 80% and 70% amino acid identity to each other, respectively; the LKCV PB1 fragment shared 83% amino acid identity with the corresponding region of QRFV PB1. Based on phylogenetic analyses, virion ultrastructural features, and the unique end-terminal sequences identified, we propose that QRFV, JAV, and LKCV comprise a novel genus of the family Orthomyxoviridae.

  7. Quaranfil, Johnston Atoll, and Lake Chad Viruses Are Novel Members of the Family Orthomyxoviridae▿

    PubMed Central

    Presti, Rachel M.; Zhao, Guoyan; Beatty, Wandy L.; Mihindukulasuriya, Kathie A.; Travassos da Rosa, Amelia P. A.; Popov, Vsevolod L.; Tesh, Robert B.; Virgin, Herbert W.; Wang, David

    2009-01-01

    Arboviral infections are an important cause of emerging infections due to the movements of humans, animals, and hematophagous arthropods. Quaranfil virus (QRFV) is an unclassified arbovirus originally isolated from children with mild febrile illness in Quaranfil, Egypt, in 1953. It has subsequently been isolated in multiple geographic areas from ticks and birds. We used high-throughput sequencing to classify QRFV as a novel orthomyxovirus. The genome of this virus is comprised of multiple RNA segments; five were completely sequenced. Proteins with limited amino acid similarity to conserved domains in polymerase (PA, PB1, and PB2) and hemagglutinin (HA) genes from known orthomyxoviruses were predicted to be present in four of the segments. The fifth sequenced segment shared no detectable similarity to any protein and is of uncertain function. The end-terminal sequences of QRFV are conserved between segments and are different from those of the known orthomyxovirus genera. QRFV is known to cross-react serologically with two other unclassified viruses, Johnston Atoll virus (JAV) and Lake Chad virus (LKCV). The complete open reading frames of PB1 and HA were sequenced for JAV, while a fragment of PB1 of LKCV was identified by mass sequencing. QRFV and JAV PB1 and HA shared 80% and 70% amino acid identity to each other, respectively; the LKCV PB1 fragment shared 83% amino acid identity with the corresponding region of QRFV PB1. Based on phylogenetic analyses, virion ultrastructural features, and the unique end-terminal sequences identified, we propose that QRFV, JAV, and LKCV comprise a novel genus of the family Orthomyxoviridae. PMID:19726499

  8. West African monsoon dynamics inferred from abrupt fluctuations of Lake Mega-Chad

    PubMed Central

    Armitage, Simon J.; Bristow, Charlie S.; Drake, Nick A.

    2015-01-01

    From the deglacial period to the mid-Holocene, North Africa was characterized by much wetter conditions than today. The broad timing of this period, termed the African Humid Period, is well known. However, the rapidity of the onset and termination of the African Humid Period are contested, with strong evidence for both abrupt and gradual change. We use optically stimulated luminescence dating of dunes, shorelines, and fluviolacustrine deposits to reconstruct the fluctuations of Lake Mega-Chad, which was the largest pluvial lake in Africa. Humid conditions first occur at ∼15 ka, and by 11.5 ka, Lake Mega-Chad had reached a highstand, which persisted until 5.0 ka. Lake levels fell rapidly at ∼5 ka, indicating abrupt aridification across the entire Lake Mega-Chad Basin. This record provides strong terrestrial evidence that the African Humid Period ended abruptly, supporting the hypothesis that the African monsoon responds to insolation forcing in a markedly nonlinear manner. In addition, Lake Mega-Chad exerts strong control on global biogeochemical cycles because the northern (Bodélé) basin is currently the world’s greatest single dust source and possibly an important source of limiting nutrients for both the Amazon Basin and equatorial Atlantic. However, we demonstrate that the final desiccation of the Bodélé Basin occurred around 1 ka. Consequently, the present-day mode and scale of dust production from the Bodélé Basin cannot have occurred before 1 ka, suggesting that its role in fertilizing marine and terrestrial ecosystems is either overstated or geologically recent. PMID:26124133

  9. Lake drying and livelihood dynamics in Lake Chad: Unravelling the mechanisms, contexts and responses.

    PubMed

    Okpara, Uche T; Stringer, Lindsay C; Dougill, Andrew J

    2016-11-01

    This article examines lake drying and livelihood dynamics in the context of multiple stressors through a case study of the "Small Lake Chad" in the Republic of Chad. Livelihoods research in regions experiencing persistent lake water fluctuations has largely focused on the well-being and security of lakeshore dwellers. Little is known about the mechanisms through which lake drying shapes livelihood drawbacks and opportunities, and whether locally evolved responses are enhancing livelihoods. Here we address these gaps using empirical, mixed-methods field research couched within the framework of livelihoods and human well-being contexts. The analysis demonstrates that limited opportunities outside agriculture, the influx of mixed ethnic migrants and the increasing spate of violence all enhance livelihood challenges. Livelihood opportunities centre on the renewal effects of seasonal flood pulses on lake waters and the learning opportunities triggered by past droughts. Although drying has spurred new adaptive behaviours predicated on seasonality, traditional predictive factors and the availability of assets, responses have remained largely reactive. The article points to where lake drying fits amongst changes in the wider socio-economic landscape in which people live, and suggests that awareness of the particularities of the mechanisms that connect lake drying to livelihoods can offer insights into the ways local people might be assisted by governments and development actors.

  10. STS-65 Earth observation of Lake Chad, Africa, taken aboard Columbia, OV-102

    NASA Technical Reports Server (NTRS)

    1994-01-01

    STS-65 Earth observation taken aboard Columbia, Orbiter Vehicle (OV) 102, shows Lake Chad, Africa. This is another long term ecological monitoring site for NASA scientists. Lake Chad was first photographed from space in 1965. A 25-year length-of-record data set exists for this environmentally important area. A number of these scenes have been digitized, rectified, classified and results show that the lake area has been shrinking and only 15% to 20% of the surface water is visible on space images. NASA's objective in monitoring this lake is to document the intra- and interannual areal changes of the largest standing water body in the Sahelian biome of North Africa. These areal changes are an indicator of the presence or absence of drought across the arguably overpopulated, overgrazed, and over biological carrying capacity limits nations of the Sahel.

  11. Fishing and farming at Lake Chad: institutions for access to natural resources.

    PubMed

    Sarch, M T

    2001-06-01

    Lake Chad is a vitally important wetland in the semi-arid Sahel corridor. It provides the basis of many thousands of livelihoods which depend on its seasonal fluctuations to renew fish stocks, farmland and rangeland. This paper describes how access to farmland and fishing rights has evolved on the Nigerian shore of the lake. The paper aims to assess the applicability of different institutional approaches to natural resource management on the lake shore. Although many recent approaches to natural resource management have reflected a 'Tragedy of the Commons' approach, a growing literature both in support and critical of Hardin's (1968) thesis has followed. Four distinct approaches are considered: (1) institutional intervention to prevent 'tragedy'; (2) institution erosion brought about by such interventions; (3) models of institutional adaptation to resource scarcity; and (4) approaches which perceive institutions as a crucial determinant of social and economic development. In examining which institutional approaches may be relevant at Lake Chad, it is anticipated that this paper will provide insights which will be relevant to policy-makers, resource managers and students working in environments where resource fluctuation inhibits stable property rights and state resource management has proved neither feasible nor successful. The western shore of Lake Chad has been under the jurisdiction of Borno State in its various guises since the end of the fourteenth century and is currently one of 36 states in the Federal Republic of Nigeria. Although the administrative status of Borno itself has varied, it has been dominated by a Kanuri aristocracy for most of its existence. The Kanuri administration has continued to operate in a remarkably similar way over the past 150 years. Traditionally, it has played a crucial role in allocating access to farm land. In recent years, the 'Kanuri administration' has not only maintained its pre-colonial authority over farming on the lake

  12. Climate Change in Africa: Impacts and Effects on the Inhabitants of the Lake Chad Region.

    NASA Astrophysics Data System (ADS)

    Abubakar, B.; Tahir, S. M.; Olisa, O.

    2009-05-01

    The Department of Energy and Climate Change defined climate as the average weather experienced over a long period. This includes temperature, wind and rainfall patterns. The climate of the Earth is not static, and has changed many times in response to a variety of natural causes. Due to human activities in emmiting green house gases has resulted the Earth to get warmed by 0.74°C over the last hundred years. Around 0.4°C of this warming has occurred since the 1970s. Climate is now one of the major phenomenon threatening lives and humanity in general since the beginning of industrial revolution. Climate exerts a profound influence on the lives of poor populations in the Lake Chad region of Africa who depend on fishing and crop cultivation for livelihood and sustenance, who are unprotected against climate-related diseases, who lacked secure access to water and food and who are vulnerable to hydro meteorological hazard. The effects of climate change on the study area are many and include diminishing resources and conflicts over the available limited water resources. The Lake Chad region is a fragile area with high climate variability and extremes of weather. As this inland water is used for domestic and agricultural purposes, salt mining, as well as transportation by Nigerians, Nigeriens, Chadian and Cameroonians, it is an area of trans-boundary water conflicts. This paper examines the part played by climate change in the decline of fishery resources and livelihood activities in the Lake Chad region. Data from field studies, structured interview and secondary sources show that fish catches and livelihood activities have declined tremendously in recent times due to several factors including overexploitation and increasing demands on the aquatic resources. Findings from the study show that droughty periods have resulted in the reduction of open lake water surface from about 25,000 km2 in 1973 to less than 2,000 km2 in the 1990s. This has led to the diminishing aquatic

  13. Coherent monsoonal changes in the northern tropics revealed by Chadian lakes (L. Chad and Yoa) sedimentary archives during the African Humid Period

    NASA Astrophysics Data System (ADS)

    Sylvestre, Florence; Kroepelin, Stefan; Pierre, Deschamps; Christine, Cocquyt; Nicolas, Waldmann; Kazuyo, Tachikawa; Amaral Paula, Do; Doriane, Delanghe; Guillaume, Jouve; Edouard, Bard; Camille, Bouchez; Jean-Claude, Doumnang; Jean-Charles, Mazur; Martin, Melles; Guillemette, Menot; Frauke, Rostek; Nicolas, Thouveny; Volkner, Wennrich

    2016-04-01

    In northern African tropics, it is now well established that the Last Glacial Maximum (LGM) was extremely dry followed by a wetter Holocene. Numerous palaeolake records reveal a fairly consistent pattern of a moister early Holocene resulting in a green Sahara followed by the onset of aridification about 4000 years ago. These palaeoenvironmental conditions are deciphered from several continental records distributed over the sub-Saharan zone and including diverse environments. However, pronounced differences in the timing and amplitude of these moisture changes inferred from sedimentary records point to both regional climatic variability change and site-specific influences of local topographic-hydrogeological factors which biased the evolution of water balance reconstructed from individual lacustrine archives. Here we present hydrological reconstructions from Chadian lakes, i.e. Lake Chad (c. 13°N) and Lake Yoa (19°N). Because of their location, both records allow to reconstruct lake level fluctuations and environmental changes according to a gradient from Sahelian to Saharan latitudes. Whereas Lake Chad is considered as a good sensor of climatic changes because of its large drainage basin covering 610,000 km2 in the Sudanian belt, Lake Yoa logs the northern precipitation changes in the Sahara. Combining sedimentological (laser diffraction grain size) and geochemical (XRF analysis) data associated with bio-indicators proxies (diatoms, pollen), we compare lake-level fluctuations and environmental changes during the last 12,000 years. After the hyperarid Last Glacial Maximum period during which dunes covered the Lake Chad basin, both lake records indicate an onset of more humid conditions between 12.5-11 ka cal BP. These resulted in lacustrine transgressions approaching their maximum extension at c. 10.5 ka cal BP. The lacustrine phase was probably interrupted by a relatively short drying event occurring around 8.2 ka cal BP which is well-defined in Lake Yoa by

  14. Chad.

    PubMed

    1988-08-01

    Focus in this discussion of Chad is on the following: geography; the people; history; government and political conditions; the economy; foreign relations; and relations between Chad and the US. In 1987 the population was estimate to be 4.5 million with an annual growth rate of 2.5%. Chad is the largest country of former French Equatorial Africa. There are more than 200 ethnic groups in Chad. Ancient Arab manuscripts and modern archeological investigations document that well-developed societies flourished around Lake Chad over 1000 years ago. The French first penetrated Chad in 1891, establishing their authority through military expeditions. In 1959, the territory of French Equatorial Africa was dissolved and 4 states -- Gabon, the Central African Republic, Congo (Brazzaville), and Chad -- became autonomous members of the French Community. In 1960, Chad became an independent nation under its 1st president, Francois Tombalbaye. The Chadian government, announced in October 1982, is organized according to the Fundamental Act proclaimed by the Command Council of the Armed Forces of the North (CCFAN). The act provides for a president and head of state, a Council of Ministers, and a 30-member National Consultative Council. President Habre has sought to bring about national reconciliation by winning the allegiance of Chad's disaffected groups. He has offered any opponents or exiles who want to rejoin the Chadian polity the opportunity to do so without fear of recrimination or punishment. The primary political vehicle for the Habre government, the National Union for Independence and Revolution (UNIR), was established in June 1984, and efforts are underway to enlist members into UNIR and establish committees from the village and block level through regional committees. Most of the population earns their living from subsistence agriculture, fishing, and stock raising, with past Chadian participation in these essentially subsistence activities reaching 96%. Cotton has

  15. Satellite-based observations of rain-induced NOx emissions from soils around Lake Chad in the Sahel

    NASA Astrophysics Data System (ADS)

    Zörner, Jan; Penning de Vries, Marloes; Dörner, Steffen; Sihler, Holger; Beirle, Steffen; Wagner, Thomas

    2017-04-01

    Rain-induced emission pulses of NOx (≡ NO + NO2) from soils have been observed in many semi-arid regions over the world. They are induced by the first precipitation of the wet season and are mainly caused by the sudden re-activation of microbes in the soil releasing reactive nitrogen. In this study, a single intense event of pulsed NOx emissions from soils around Lake Chad is investigated. This is achieved by analysing daily tropospheric NO2 vertical column densities (VCDs) as observed by the satellite-based OMI instrument together with other satellite and model data on precipitation, lightning, fire and wind. The study region of Lake Chad and its ecosystems are indispensable to life in the Sahel region. Climate variability and unsustainable water utilization, however, caused a drastic decrease in the lakes' surface area which, in turn, lead to extensive land cover changes converting former lake area to shrub land and fertile farm land. The results indicate that the region of Lake Chad does not only show consistent enhancements in average NO2 VCDs in the early months of the wet season compared to its surrounding desert but also exhibits particularly strong NOx emissions shortly after a single large-scale precipitation event in June 2007. NO2 VCDs measured 14 hours after this precipitation event show strong enhancements (2.5*1015 molecules cm-2) compared to the seasonal background VCDs and, moreover, represent the highest detected NO2 VCDs of the entire year. Detailed analysis of potential contributors to the observed NO2 VCDs strongly indicate that fire, lightning and retrieval artefacts cannot explain the NO2 pulse. The estimated emission flux from the soil, calculated based on mass balance, amounts to about 32.3 ng N m-2 s-1, which corresponds to about 65 tonnes of nitrogen released to the atmosphere within one day.

  16. The prevalence of bovine venereal campylobacteriosis in cattle herds in the Lake Chad basin of Nigeria.

    PubMed

    Mshelia, Gideon Dauda; Amin, Jibrilla Dahiru; Egwu, Godwin Onyeamaechi; Woldehiwet, Zerai; Murray, Richard Donald

    2012-10-01

    The prevalence of bovine venereal campylobacteriosis (BVC) was investigated in the Lake Chad basin of Nigeria. Preputial washings and cervico-vaginal mucus samples were obtained from 270 cattle presenting a history of abortion and lowered fertility, kept in traditional and institutional farms. All the samples investigated were cultured using standard bacteriological technique. Campylobacter fetus was isolated from six bulls and four cows. In all cattle sampled, the isolation rates were 2.2% for C. fetus subsp. venerealis and 1.5% for C. fetus subsp. fetus; the herd and within-herd prevalence rates for C. fetus were 22.2% and 3.4%, respectively, while the overall active infectivity rate was 3.7%. BVC probably contributes to lowered fertility and abortions found in cattle in the Lake Chad basin of Nigeria, associated more with C. fetus subsp. venerealis than C. fetus subsp. fetus.

  17. Lake Chad sedimentation and environments during the late Miocene and Pliocene: New evidence from mineralogy and chemistry of the Bol core sediments

    NASA Astrophysics Data System (ADS)

    Moussa, Abderamane; Novello, Alice; Lebatard, Anne-Elisabeth; Decarreau, Alain; Fontaine, Claude; Barboni, Doris; Sylvestre, Florence; Bourlès, Didier L.; Paillès, Christine; Buchet, Guillaume; Duringer, Philippe; Ghienne, Jean-François; Maley, Jean; Mazur, Jean-Charles; Roquin, Claude; Schuster, Mathieu; Vignaud, Patrick; Brunet, Michel

    2016-06-01

    This study presents mineralogical and geochemical data from a borehole drilled near the locality of Bol (13°27‧N, 14°44‧E), in the eastern archipelago of the modern Lake Chad (Chad). Samples were taken from a ∼200 m long core section forming a unique sub-continuous record for Central Africa. Among these samples, 25 are dated between 6.4 and 2.4 Ma. Dominant minerals are clays (66% average) mixed with varying amounts of silt and diatomite. The clay fraction consists of Fe-beidellite (87% average), kaolinite, and traces of illite. Clay minerals originate from the erosion of the vertisols that surrounded the paleolake Chad. Sedimentological data indicate that a permanent lake (or recurrent lakes) existed from 6.7 until 2.4 Ma in the vicinity of Bol. By comparison with modern latitudinal distribution of vertisols in Africa the climate was Sudanian-like. Changes in the sedimentation rate suggest a succession of wetter and dryer periods during at least six million years in the region during the critical time period covering the Miocene-Pliocene transition.

  18. Advanced Land Use Classification for Nigeriasat-1 Image of Lake Chad Basin

    NASA Astrophysics Data System (ADS)

    Babamaaji, R.; Park, C.; Lee, J.

    2009-12-01

    Lake Chad is a shrinking freshwater lake that has been significantly reduced to about 1/20 of its original size in the 1960’s. The severe draughts in 1970’s and 1980’s and following overexploitations of water resulted in the shortage of surface water in the lake and the surrounding rivers. Ground water resources are in scarcity too as ground water recharge is mostly made by soil infiltration through soil and land cover, but this surface cover is now experiencing siltation and expansion of wetland with invasive species. Large changes in land use and water management practices have taken place in the last 50 years including: removal of water from river systems for irrigation and consumption, degradation of forage land by overgrazing, deforestation, replacing natural ecosystems with mono-cultures, and construction of dams. Therefore, understanding the change of land use and its characteristics must be a first step to find how such changes disturb the water cycle around the lake and affect the shrinkage of the lake. Before any useful thematic information can be extracted from remote sensing data, a land cover classification system has to be developed to obtain the classes of interest. A combination of classification systems used by Global land cover, Water Resources eAtlass and Lake Chad Basin Commission gave rise to 7 land cover classes comprising of - Cropland, vegetation, grassland, water body, shrub-land, farmland ( mostly irrigated) and bareland (i.e. clear land). Supervised Maximum likelihood classification method was used with 15 reference points per class chosen. At the end of the classification, the overall accuracy is 93.33%. Producer’s accuracy for vegetation is 40% compare to the user’s accuracy that is 66.67 %. The reason is that the vegetation is similar to shrub land, it is very hard to differentiate between the vegetation and other plants, and therefore, most of the vegetation is classified as shrub land. Most of the waterbodies are occupied

  19. Chad Basin: Paleoenvironments of the Sahara since the Late Miocene

    NASA Astrophysics Data System (ADS)

    Schuster, Mathieu; Duringer, Philippe; Ghienne, Jean-François; Roquin, Claude; Sepulchre, Pierre; Moussa, Abderamane; Lebatard, Anne-Elisabeth; Mackaye, Hassan Taisso; Likius, Andossa; Vignaud, Patrick; Brunet, Michel

    2009-08-01

    Since the mid 1990s, the Mission paléoanthropologique francotchadienne (MPFT) conducts yearly paleontological field investigations of the Miocene-Pliocene of the Chad Basin. This article synthesizes some of the results of the MPFT, with focus on the Chad Basin development during the Neogene. We propose an overview of the depositional paleoenvironments of this part of Africa at different scales of time and space, based on a multidisciplinary approach (sedimentary geology, geomorphology, geophysic, numerical simulations and geochronology). The Miocene-Pliocene paleoenvironments are examined through the sedimentary archives of the early hominids levels and the Holocene Lake Mega-Chad episode illustrates the last major paleoenvironmental change in this area. The sedimentary record of the Chad Basin since the Late Miocene can be schematized as the result of recurrent interactions from lake to desert environments.

  20. Characteristics of Lake Chad Level Variability and Links to ENSO, Precipitation, and River Discharge

    PubMed Central

    Demoz, Belay; Gebremariam, Sium

    2014-01-01

    This study used trend, correlation, and wavelet analysis to characterize Lake Chad (LC) level fluctuations, river discharge, El Niño Southern Oscillation (ENSO), and precipitation regimes and their interrelationships. Linear correlation results indicate a negative association between ENSO and LC level, river discharge and precipitation. Trend analysis shows increasing precipitation in the Lake Chad Basin (LCB) but decreasing LC level. The mode of interannual variability in LC level, rainfall, and ENSO analyzed using wavelet analysis is dominated by 3-4-year periods. Results show that variability in ENSO could explain only 31% and 13% of variations in LC level at Kindjeria and precipitation in the northern LCB, respectively. The wavelet transform coherency (WTC) between LC level of the southern pool at Kalom and ENSO is statistically significant at the 95% confidence level and phase-locked, implying a cause-and-effect association. These strong coherencies coincide with the La Niña years with the exception of 1997-1998 El Niño events. The WTC shows strong covariance between increasing precipitation and LC level in the northern pool at a 2- to 4-year band and 3- to 4-year band localized from 1996 to 2010. Implications for water resource planning and management are discussed. PMID:25538946

  1. Spatio-temporal variability of droughts and terrestrial water storage over Lake Chad Basin using independent component analysis

    NASA Astrophysics Data System (ADS)

    Ndehedehe, Christopher E.; Agutu, Nathan O.; Okwuashi, Onuwa; Ferreira, Vagner G.

    2016-09-01

    Lake Chad has recently been perceived to be completely desiccated and almost extinct due to insufficient published ground observations. Given the high spatial variability of rainfall in the region, and the fact that extreme climatic conditions (for example, droughts) could be intensifying in the Lake Chad basin (LCB) due to human activities, a spatio-temporal approach to drought analysis becomes essential. This study employed independent component analysis (ICA), a fourth-order cumulant statistics, to decompose standardised precipitation index (SPI), standardised soil moisture index (SSI), and terrestrial water storage (TWS) derived from Gravity Recovery and Climate Experiment (GRACE) into spatial and temporal patterns over the LCB. In addition, this study uses satellite altimetry data to estimate variations in the Lake Chad water levels, and further employs relevant climate teleconnection indices (El-Niño Southern Oscillation-ENSO, Atlantic Multi-decadal Oscillation-AMO, and Atlantic Meridional Mode-AMM) to examine their links to the observed drought temporal patterns over the basin. From the spatio-temporal drought analysis, temporal evolutions of SPI at 12 month aggregation show relatively wet conditions in the last two decades (although with marked alterations) with the 2012-2014 period being the wettest. In addition to the improved rainfall conditions during this period, there was a statistically significant increase of 0.04 m/yr in altimetry water levels observed over Lake Chad between 2008 and 2014, which confirms a shift in the hydrological conditions of the basin. Observed trend in TWS changes during the 2002-2014 period shows a statistically insignificant increase of 3.0 mm/yr at the centre of the basin, coinciding with soil moisture deficit indicated by the temporal evolutions of SSI at all monthly accumulations during the 2002-2003 and 2009-2012 periods. Further, SPI at 3 and 6 month scales indicated fluctuating drought conditions at the extreme south

  2. Biomass burning and its relationship with water cycle dynamics of the Chari-Logone catchment of Lake Chad Basin

    NASA Astrophysics Data System (ADS)

    Black, F. W.; Lee, J.; Ellison, L.; Gupta, M.; Bolten, J. D.; Gatebe, C. K.; Ichoku, C. M.

    2016-12-01

    The cause of shrinkage of Lake Chad has been of great interest for issues of global warming and climate change. The present study investigates the effect of biomass burning on the water cycle dynamics of Lake Chad Basin in the Northern Sub-Saharan Africa. Burning activities increase from November to April when monsoonal precipitation is at its lowest and decreases dramatically from May to October when precipitation peaks. To circumvent weather station scarcity in the region, a variety of satellite products were used as input into a water balance model. The datasets include TRMM 3B31 for precipitation, SRTM for elevation, and MODIS: MOD11C3 for temperature, MOD12Q1 for land cover, and MOD14A for fire count. Non-satellite based data sources include soil maps from the Harmonized World Soil Database and wind speed from NOAA NCDC stations. The Chari-Logone catchment of the Lake Chad Basin was selected since it supplies over 90% of the water input to the Lake. Fire count data from MOD14A were integrated with land cover albedo changes to determine monthly potential evapotranspiration (PET) using a Penman equation. The resolution of the model is 2 km x 2 km which allows for delineation of physical features such as lakes and other water bodies. Fire counts, also at a resolution of 2 km x 2 km, vary dramatically depending on the season. A separate land cover dataset was created to account for the effect of burning of different vegetative land types, which affects vegetative area, bare area, leaf area index, vegetation height, Manning coefficient, and aerodynamic resistance. Two water balance simulations, one considering burning and one without, were compared from the years 2005 to 2010. Results indicate biomass burning contribute to an increase in average monthly runoff and a decrease in groundwater recharge. Actual evapotranspiration shows variation depending on the month.

  3. Remote sensing appraisal of Lake Chad shrinkage connotes severe impacts on green economics and socio-economics of the catchment area.

    PubMed

    Onamuti, Olapeju Y; Okogbue, Emmanuel C; Orimoloye, Israel R

    2017-11-01

    Lake Chad commonly serves as a major hub of fertile economic activities for the border communities and contributes immensely to the national growth of all the countries that form its boundaries. However, incessant and multi-decadal drying via climate change pose greater threats to this transnational water resource, and adverse effects on ecological sustainability and socio-economic status of the catchment area. Therefore, this study assessed the extent of shrinkage of Lake Chad using remote sensing. Landsat imageries of the lake and its surroundings between 1987 and 2005 were retrieved from Global Land Cover Facility website and analysed using Integrated Land and Water Information System version 3.3 (ILWIS 3.3). Supervised classification of area around the lake was performed into various land use/land cover classes, and the shrunk part of its environs was assessed based on the land cover changes. The shrinkage trend within the study period was also analysed. The lake water size reduced from 1339.018 to 130.686 km 2 (4.08-3.39%) in 1987-2005. The supervised classification of the Landsat imageries revealed an increase in portion of the lake covered by bare ground and sandy soil within the reference years (13 490.8-17 503.10 km 2 ) with 4.98% total range of increase. The lake portion intersected with vegetated ground and soil also reduced within the period (11 046.44-10 078.82 km 2 ) with 5.40% (967.62 km 2 ) total decrease. The shrunk part of the lake covered singly with vegetation increased by 2.74% from 1987 to 2005. The shrunk part of the lake reduced to sand and turbid water showed 5.62% total decrease from 1987 to 2005 and a total decrease of 1805.942 km 2 in area. The study disclosed an appalling rate of shrinkage and damaging influences on the hydrologic potential, eco-sustainability and socio-economics of the drainage area as revealed using ILWIS 3.3.

  4. Viruses in Antarctic lakes

    NASA Technical Reports Server (NTRS)

    Kepner, R. L. Jr; Wharton, R. A. Jr; Suttle, C. A.; Wharton RA, J. r. (Principal Investigator)

    1998-01-01

    Water samples collected from four perennially ice-covered Antarctic lakes during the austral summer of 1996-1997 contained high densities of extracellular viruses. Many of these viruses were found to be morphologically similar to double-stranded DNA viruses that are known to infect algae and protozoa. These constitute the first observations of viruses in perennially ice-covered polar lakes. The abundance of planktonic viruses and data suggesting substantial production potential (relative to bacteria] secondary and photosynthetic primary production) indicate that viral lysis may be a major factor in the regulation of microbial populations in these extreme environments. Furthermore, we suggest that Antarctic lakes may be a reservoir of previously undescribed viruses that possess novel biological and biochemical characteristics.

  5. CHAD Explorer - An Enhanced Web Application for CHAD

    EPA Science Inventory

    In 1999 the US Environmental Protection Agency (EPA) developed a Consolidated Human Activity Database (CHAD). CHAD has been used for various exposure modeling studies (McCurdy et al., 2000, Graham & McCurdy, 2004; McCurdy & Graham, 2003). Recently, CHAD has been improved and enha...

  6. Re-infection with Fasciola gigantica 6-month post-treatment with triclabendazole in cattle from mobile pastoralist husbandry systems at Lake Chad.

    PubMed

    Greter, Helena; Batil, Annour A; Alfaroukh, Idriss O; Grimm, Felix; Ngandolo, Bongo N; Keiser, Jennifer; Utzinger, Jürg; Zinsstag, Jakob; Hattendorf, Jan

    2016-10-30

    At Lake Chad in central Africa, livestock fascioliasis caused by Fasciola gigantica represents a major veterinary health problem, particularly in cattle reared in mobile pastoralist husbandry systems. We assessed re-infection after a single dose of triclabendazole with fascioliasis in cattle in a mobile pastoralist setting towards the end of the dry season. Within the cattle herds of 14 groups of mobile pastoralists, 375 cattle were randomly selected. A faecal sample was obtained from each animal to determine the prevalence of F. gigantica. Animals were administered a single oral dose of triclabendazole (12mg/kg). A second faecal sample was obtained 6-month post-treatment after cattle had returned from the annual migration cycle. Faecal samples were fixed in sodium acetate-acetic acid-formalin (SAF), and examined for F. gigantica using the sedimentation technique. From the 375 cattle enrolled at baseline, 198 animals (53%) in 12 groups of mobile pastoralists were re-sampled at the 6-month follow-up. Baseline prevalence did not differ noteworthy between animals lost to follow-up and those re-examined. At baseline, bovine fascioliasis prevalence in cattle with follow-up data was 41.9% (95% confidence interval (CI) 35.2-48.9%). At the 6-month post-treatment follow-up, the prevalence was 46.0% (95% CI 39.2-52.9%), ranging between 0% and 75% at the herd level. The mean faecal egg counts at the unit of the herd were higher at follow-up compared to baseline. The observed persistent high prevalence of F. gigantica infection in cattle shows that a single pre-rainy season treatment does not prevent rapid re-infection despite the partial migration away from the high-risk areas at Lake Chad into drier areas. A locally adapted strategic control package for fascioliasis in cattle in the Lake Chad area ought to integrate targeted triclabendazole treatment and seasonal transhumance practices. Copyright © 2016 Elsevier B.V. All rights reserved.

  7. Genetic heterogeneity of hepatitis E virus in Darfur, Sudan, and neighboring Chad.

    PubMed

    Nicand, Elisabeth; Armstrong, Gregory L; Enouf, Vincent; Guthmann, Jean Paul; Guerin, Jean-Philippe; Caron, Mélanie; Nizou, Jacques Yves; Andraghetti, Roberta

    2005-12-01

    The within-outbreak diversity of hepatitis E virus (HEV) was studied during the outbreak of hepatitis E that occurred in Sudan in 2004. Specimens were collected from internally displaced persons living in a Sudanese refugee camp and two camps implanted in Chad. A comparison of the sequences in the ORF2 region of 23 Sudanese isolates and five HEV samples from the two Chadian camps displayed a high similarity (>99.7%) to strains belonging to Genotype 1. But four isolates collected in one of the Chadian camps were close to Genotype 2. Circulation of divergent strains argues for possible multiple sources of infection. Copyright (c) 2005 Wiley-Liss, inc.

  8. High-coercivity minerals from North African Humid Period soil material deposited in Lake Yoa (Chad)

    NASA Astrophysics Data System (ADS)

    Just, J.; Kroepelin, S.; Wennrich, V.; Viehberg, F. A.; Wagner, B.; Rethemeyer, J.; Karls, J.; Melles, M.

    2015-12-01

    The Holocene is a period of fundamental climatic change in North Africa. Humid conditions during the so-called African Humid Period (AHP) have favored the formation of big lake systems. Only very few of these lakes persist until today. One of them is Lake Yoa (19°03'N/20°31'E) in the Ounianga Basin, Chad, which maintains its water level by ground water inflow. Here we present the magnetic characteristics together with proxies for lacustrine productivity and biota of a sediment core (Co1240) from Lake Yoa, retrieved in 2010 within the framework of the Collaborative Research Centre 806 - Our Way to Europe (Deutsche Forschungsgemeinschaft). Magnetic properties of AHP sediments show strong indications for reductive diagenesis. An up to ~ 80 m higher lake level is documented by lacustrine deposits in the Ounianga Basin, dating to the early phase of the AHP. The higher lake level and less strong seasonality restricted deep mixing of the lake. Development of anoxic conditions consequently lead to the dissolution of iron oxides. An exception is an interval with high concentration of high-coercivity magnetic minerals, deposited between 7800 - 8120 cal yr BP. This interval post-dates the 8.2 event, which was dry in Northern Africa and probably caused a reduced vegetation cover. We propose that the latter resulted in the destabilization of soils around Lake Yoa. After the re-establishment of humid conditions, these soil materials were eroded and deposited in the lake. Magnetic minerals appear well preserved in the varved Late Holocene sequence, indicating (sub-) oxic conditions in the lake. This is surprising, because the occurrence of varves is often interpreted as an indicator for anoxic conditions of the lake water. However, the salinity of lake water rose strongly after the AHP. We therefore hypothesize that the conservation of varves and absence of benthic organisms rather relates to the high salinity than to anoxic conditions.

  9. Chad: World Oil Report 1991

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    1991-08-01

    This paper reports on Mango 1, which is an exploration well started in September 1990 on a block adjacent to Lake Chad by Esso, Chevron and Shell was suspended after the coup in that nation's capital later in the year. The small Sedigi oil field, discovered in the 70s, will be developed with a pipeline to a 3,000-bpd refinery. Improved relations with Libya and future internal stability may further open the door to exploration.

  10. Dust storm in Chad

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Lake Chad (lower left) and the surrounding wetlands are under increasing pressure from desertification. The encroachment of the Sahara occurs with creeping sand dunes and major dust storms, such as the one pictured in this MODIS image from October 28, 2001. The amount of open water (lighter green patch within the darker one) has declined markedly over the last decades and the invasion of dunes is creating a rippled effect through the wetlands that is all too clear in the high-resolution images. Growing population and increasing demands on the lake give it an uncertain future. The loss of such an important natural resource will have profound effects on the people who depend on the rapidly diminishing source of fresh water. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  11. CHAD USER’S GUIDE: Extracting Human Activity Information from CHAD on the PC

    EPA Science Inventory

    The Consolidated Human Activity Database (CHAD) User Guide offers a short tutorial about CHAD Access; background on the CHAD Databases; background on individual studies in CHAD; and information about using CHAD data, caveats, known problems, notes, and database design and develop...

  12. The origin of Late Ediacaran post-collisional granites near the Chad Lineament, Saharan Metacraton, South-Central Chad

    NASA Astrophysics Data System (ADS)

    Shellnutt, J. Gregory; Yeh, Meng-Wan; Lee, Tung-Yi; Iizuka, Yoshiyuki; Pham, Ngoc Ha T.; Yang, Chih-Cheng

    2018-04-01

    The southern Saharan Metacraton is one of the least geologically constrained regions in the world as bedrock exposures are rare. To the west of Lake Fitri near the communities of Ngoura and Moyto in south-central Chad there are two granitic inliers that form a series of lenticular to ellipsoidal low laying hills. Very little is known about the Lake Fitri inliers and their regional correlation to larger massifs in Chad is undetermined. The granites yielded weighted-mean zircon 206Pb/238U ages of 554 ± 8 Ma and 546 ± 8 Ma indicating they were emplaced 45 million years after the cessation of arc-related magmatism and the subsequent collision between the Congo-São Francisco Craton and the Saharan Metacraton. The rocks have distinct groupings of inherited zircons with ages of 580 Ma and 635 Ma suggesting they are at least in part derived by recycling of older crustal rocks. The biotite mineral chemistry, whole rock compositions and petrological modeling indicate the granites were derived by melting of crustal lithologies but the whole rock Nd isotopes (εNd(t) = +1.3-+2.9) are characteristic of a mantle source. The contrasting inheritance-rich nature of the granites with a juvenile Nd isotopic signature is likely due to mixing between magmas derived from juvenile (Neoproterozoic) arc-related crust and asthenospheric magmas. Asthenospheric upwelling was probably a response to post-orogenic lithospheric delamination related to fault movement along the Chad Lineament, a possible extension of the Tcholliré-Banyo shear zone that extended to the interior of the Saharan Metacraton. The implications are that lithospheric delamination may not have occurred immediately after collision but rather propagated along a narrow belt that extended well into the central regions of the Saharan Metacraton.

  13. CHAD User's Guide: Extracting Human Activity Information from CHAD on the PC

    EPA Pesticide Factsheets

    User manual that includes tutorials, what's inside the CHAD databases, background on individuals studies in CHAD, using data form individual studies, caveats, problems, notes, and database design and development.

  14. Water balance-based estimation of groundwater recharge in the Lake Chad Basin

    NASA Astrophysics Data System (ADS)

    Babamaaji, R. A.; Lee, J.

    2012-12-01

    Lake Chad Basin (LCB) has experienced drastic changes of land cover and poor water management practices during the last 50 years. The successive droughts in the 1970s and 1980s resulted in the shortage of surface water and groundwater resources. This problem of drought and shortage of water has a devastating implication on the natural resources of the Basin with great consequence on food security, poverty reduction and quality of life of the inhabitants in the LCB. Therefore, understanding the change of land use and its characteristics must be a first step to find how such changes disturb the water cycle especially the groundwater in the LCB. The abundance of groundwater is affected by the climate change through the interaction with surface water, such as lakes and rivers, and vertical recharge through an infiltration process. Quantifying the impact of climate change on the groundwater resource requires not only reliable forecasting of changes in the major climatic variables, but also accurate estimation of groundwater recharge. Spatial variations in the land use/land cover, soil texture, topographic slope, and meteorological conditions should be accounted for in the recharge estimation. In this study, we employed a spatially distributed water balance model WetSpass to simulate a long-term average change of groundwater recharge in the LCB of Africa. WetSpass is a water balance-based model to estimate seasonal average spatial distribution of surface runoff, evapotranspiration, and groundwater recharge. The model is especially suitable for studying the effect of land use/land cover change on the water regime in the LCB. The present study describes the concept of the model and its application to the development of recharge map of the LCB.

  15. Occurrence of 1,1'-dimethyl-4,4'-bipyridinium (Paraquat) in irrigated soil of the Lake Chad Basin, Niger.

    PubMed

    Crampon, Marc; Copard, Yoann; Favreau, Guillaume; Raux, Julie; Merlet-Machour, Nadine; Le Coz, Mathieu; Ibrahim, Maïmouna; Peulon-Agasse, Valérie; Portet-Koltalo, Florence

    2014-09-01

    Increased use of agrochemical products to improve yields for irrigated crops in sub-Saharan Africa has been accompanied by a significant increase in the risk of environmental contamination. Detailed examples of the fate of pesticides after initial spreading on crop fields are scarce in tropical regions, where safe practices and related health risks are poorly understood by smallholder farmers. In the semi-arid environment of the Lake Chad Basin, SE Niger, both intrinsic properties of pesticides and extrinsic factors such as soil and climate helped to characterize processes leading to an accumulation of pesticides in soils. Analysis by HPLC-UV of a 6 m deep soil profile showed the presence of Paraquat at concentrations from 953 ± 102 μg kg(-1) to 3083 ± 175 μg kg(-1) at depths between 0.80 and 2.75 m below the land surface. Soil analysis revealed that up to approximately 15 % of the total soil matrix consists of smectites, a clay mineral capable of retaining cationic pesticides such as Paraquat, and a very low content of organic matter (<0.15 wt.% TOC). Paraquat could be stored and not bioavailable in a clayey barrier at approximately 2-m depth and therefore does not represent an immediate risk for populations or environment in this form. However, if the Paraquat application rate remains constant, the clayey barrier could reach a saturation limit within 150-200 years and 180-220 years if we consider a DT50 in soil of ~1,000 days (FAO). Consequently, it could lead to a deeper infiltration and so a pollution of groundwater. Such a scenario can represent a health risk for drinking water and for the Lake Chad, which is a major resource for this densely populated region of semi-arid Africa. Further analyses should focus on deeper layers and groundwater Paraquat contents to validate or invalidate the hypothesis of storage in this clay-rich layer.

  16. Lake Yoa (Northern Chad): A Seasonal Footprint of 10,500 Years of Climate Change in the Sahara

    NASA Astrophysics Data System (ADS)

    Kroepelin, S.; Cocquyt, C.; Darius, F.; Dinies, M.; Francus, P.; Just, J.; Karls, J.; Kuper, J.; Lézine, A. M.; Mallaye, B.; Melles, M.; Sylvestre, F.; Viehberg, F. A.; Wennrich, V.

    2016-12-01

    We present Africa's most complete Holocene climate record in a long awaited breakthrough that few would have expected in one of the driest and most remote parts of the Sahara, the planet's major hot desert. A 16 m thick continuous sequence of seasonally laminated (varved) deposits at the bottom of a now fully groundwater-supported oasis lake at Ounianga Kebir in northern Chad extends our earlier 6,000 year record published in 2008 back to the onset of postglacial humid conditions 10,500 years ago in unrivalled detail. Main results indicate a rather slow regreening in northern Africa after 100,000 years of apparently continuous late Pleistocene aridity; precisely define the severe environmental impact of global climate events such as the 8,200 BP North Atlantic cooling even in hypercontinental positions far away from the oceans; and corroborate the gradual termination of the last "Green Sahara" period over millennia. Lake Yoa's varve count-controlled age model also shows the high error potential of the existing 14C chronology from bulk carbonate-dated paleolacustrine archives elsewhere in the Sahara and provides a basis for its correction. The new terrestrial multiproxy data set discloses agreements and discrepancies to marine and ice core data, and numeric climate models. As a natural analogue, it helps to foresee how North Africa's climate and environments might evolve due to anthropogenic global warming.

  17. Occurrence of water-borne enteric viruses in two settlements based in Eastern Chad: analysis of hepatitis E virus, hepatitis A virus and human adenovirus in water sources.

    PubMed

    Guerrero-Latorre, Laura; Carratala, Anna; Rodriguez-Manzano, Jesus; Calgua, Byron; Hundesa, Ayalkibet; Girones, Rosina

    2011-09-01

    Hepatitis E virus (HEV) is a common cause of water-borne acute hepatitis in areas with poor sanitation. In 2004 an outbreak of HEV infection affected around 2,000 people in Eastern Chad (Dar Sila). This paper describes the decrease in the incidence of acute jaundice syndrome (AJS) from 2004 until 2009 when a mean incidence of 0.48 cases/1,000 people/year was recorded in the region. Outbreaks of AJS were identified in some of the camps in 2007 and 2008. Moreover, water samples from drinking water sources were screened for human adenoviruses considered as viral indicators and for hepatitis A virus and HEV. Screening of faecal samples from donkeys for HEV gave negative results. Some of the samples were also analysed for faecal coliforms showing values before disinfection treatment between 3 and >50 colony forming units per 100 mL. All water samples tested were negative for HEV and HAV; however, the presence of low levels of human adenoviruses in 4 out of 16 samples analysed indicates possible human faecal contamination of groundwater. Consequently, breakdowns in the treatment of drinking water and/or increased excretion of hepatitis viruses, which could be related to the arrival of a new population, could spread future outbreaks through drinking water.

  18. Ground-water hydrology of the Chad Basin in Bornu and Dikwa Emirates, northeastern Nigeria, with special emphasis on the flow life of the artesian system

    USGS Publications Warehouse

    Miller, Raymond E.; Johnston, R.H.; Olowu, J.A.I.; Uzoma, J.U.

    1968-01-01

    Bornu and Dikwa Emirates lie in the Nigerian sector of the Chad Basin, a vast region of interior drainage encompassing about 600,000 square miles of north-central Africa. The report area includes about 25,000 square miles of the basin that lie in Nigeria. Most of the area is a featureless plain that slopes gently northeast and east from the uplands of central Nigeria towards Lake Chad. On its eastern side the lake has one surface outlet which overflows only during exceptionally high stages of the lake. This outlet spills into the channel of Bahr al Ghazal, which in turn drains into the Bod616 depression. Because the lake is shallow, the shoreline fluctuates markedly with high and low stages corresponding to the wet and dry seasons. The semiarid climate of Bornu and Dikwa Emirates is characterized by a long dry season and a short wet season that correspond to seasonal winds. Annual rainfall ranges from 15 inches in the northern part of the area to 32 inches in the southern. The Chad Basin in Dikwa and Bornu Emirates is underlain by interbedded sand and clay, collectively termed the Chad Formation. These alluvial and lactustrine sediments were deposited in or near Lake Chad whet it occupied a much greater area during Pliocene and Pleistocene time. The Chad Formation has a very slight primary dip in the direction of Lake Chad and conforms to the gentle slope of land surface. The known thickness of the formation ranges from a few feet where it overlies bedrock on the periphery of the basin to at least 1,800 feet at Maiduguri; however, its total thickness probably exceeds 2,000 feet in the central part of the basin. Three water-bearing units termed upper, middle, and lower zones occur within the Chad Formation. The upper zone yields water to numerous dug wells throughout the rural areas and also is .the major source of the Maiduguri municipal water .supply. The middle zone yields water from flowing artesian boreholes that have heads ranging from a few feet to 70 feet

  19. Outbreaks of Tilapia Lake Virus Infection, Thailand, 2015-2016.

    PubMed

    Surachetpong, Win; Janetanakit, Taveesak; Nonthabenjawan, Nutthawan; Tattiyapong, Puntanat; Sirikanchana, Kwanrawee; Amonsin, Alongkorn

    2017-06-01

    During 2015-2016, several outbreaks of tilapia lake virus infection occurred among tilapia in Thailand. Phylogenetic analysis showed that the virus from Thailand grouped with a tilapia virus (family Orthomyxoviridae) from Israel. This emerging virus is a threat to tilapia aquaculture in Asia and worldwide.

  20. CHAD-Master

    EPA Pesticide Factsheets

    Detailed data on human behavior from 19 studies has been compiled into the Consolidated Human Activity Database (CHAD) , enabling researchers to examine specific population groups for unique activity patterns that influence overall exposure to chemicals.

  1. CHAD-2000

    EPA Pesticide Factsheets

    Detailed data on human behavior from 19 studies has been compiled into the Consolidated Human Activity Database (CHAD), enabling researchers to examine specific population groups for unique activity patterns that influence overall exposure to chemicals.

  2. Storm and tide influenced depositional architecture of the Pliocene-Pleistocene Chad Formation, Chad Basin (Bornu Sub-basin) NE Nigeria: A mixed fluvial, deltaic, shoreface and lacustrine complex

    NASA Astrophysics Data System (ADS)

    Shettima, Bukar; Kyari, Aji Maina; Aji, Mallam Musa; Adams, Fatimoh Dupe

    2018-07-01

    Lithofacies analyses of the upper part of the Chad Formation (Bama Ridge Complex) in the Bornu Sub-basin of the Chad Basin indicated four facies associations; fluvial, deltaic, shoreface and lacustrine sequences. The fluvial sequences are composed of fining upward cycles with successive occurrence of planar crossbedded sandstone facies displaying unimodal paleocurrent system and rare mudstone facies typical of braided river system. The deltaic succession consists of both fining and coarsening upwards cycles with the former depicting fluvial setting of an upper delta plain while the later suggestive of mouth-bar sequences. The setting displays a polymodal current system of fluvial, waves, storms and tides that were primarily induced by complex interactions of seiches and lunar tides. Similar current systems devoid of fluvial patterns were reflected in the coarsening upward packages of the shoreface sequences. Lacustrine succession composed of thick bioturbated mudstone facies generally defines the base of these coarsening upward profiles, giving a fluvio-lacustrine geomorphic relief where complex interaction developed the deltaic and shoreface facies along its shorelines. Clay mineral fractions of the formation are dominantly kaolinitic, indicating a predominantly humid tropical-subtropical climatic condition during their deposition. This climatic regime falls within the African humid period of the early-mid Holocene that led to the third lacustrine transgression of the Lake Mega-Chad, whereas the subordinate smectite mineralization points to aridification that characterizes most of the post humid period to recent.

  3. Comparison of milk fatty acid profiles measured on Kouri cows near Lake Chad and on dairy cattle as reported by meta-analytical data.

    PubMed

    Bada Algom, O; Fabry, C; Leroy, P L; Hornick, J-L

    2017-06-01

    Kouri (Bos taurus) is a breed aboriginal from Lake Chad and threatened with extinction. This study aimed to compare milk fatty acid profiles measured on Kouri cows and on high-yielding dairy cattle in Europe and elsewhere as reported by meta-analytical data (22 experimentations). Milk samples were collected from 14 Kouri dairy cows in dry season (March to June) and fatty acids (FA) were determined by gas chromatography. Overall, 32 FA have been identified. Kouri showed lower values (P < 0.001) in the sum of saturated FA (SFA, -10.9 pts), cis-9, cis-12 18:2 (-1.00 pt) (P < 0.01, higher values (P < 0.001) in the sum of monounsaturated FA (MUFA, +15.3 pts), C18:0) (+3.5 pts), cis-9, trans-11 C18:2-CLA (+1.00 pts), trans-11 18:1 (+1.4 pts) and (P < 0.01) in cis-9, C18:1 (+3.00 pts) acids. The differences between the milk FA profile of the Kouri cows and that obtained from meta-analytical data could be the possible consequence of the use of particular lake pastures by Kouri cows.

  4. Outbreaks of Tilapia Lake Virus Infection, Thailand, 2015–2016

    PubMed Central

    Janetanakit, Taveesak; Nonthabenjawan, Nutthawan; Tattiyapong, Puntanat; Sirikanchana, Kwanrawee; Amonsin, Alongkorn

    2017-01-01

    During 2015–2016, several outbreaks of tilapia lake virus infection occurred among tilapia in Thailand. Phylogenetic analysis showed that the virus from Thailand grouped with a tilapia virus (family Orthomyxoviridae) from Israel. This emerging virus is a threat to tilapia aquaculture in Asia and worldwide. PMID:28518020

  5. Combined Effect of El Nino Southern Oscillation and Atlantic Multidecadal Oscillation on Lake Chad Level Variability Region

    NASA Technical Reports Server (NTRS)

    Okonkwo, Churchill; Demoz, Belay; Sakai, Ricardo; Ichoku, Charles; Anarado, Chigozie; Adegoke, Jimmy; Amadou, Angelina; Abdullahi, Sanusu Imran

    2015-01-01

    In this study, the combined effect of the Atlantic Multidecadal Oscillation (AMO) and El Niño Southern Oscillation (ENSO) on the Lake Chad (LC) level variability is explored. Our results show that the lake level at the Bol monitoring station has a statistically significant correlation with precipitation (R2 = 0.6, at the 99.5% confidence level). The period between the late 1960s and early 1970s marked a turning point in the response of the regional rainfall to climatic drivers, thereby severely affecting the LC level. Our results also suggest that the negative impact of the cold phase of AMO on Sahel precipitation masks and supersedes the positive effect of La Niña in the early the 1970s. The drop in the size of LC level from 282.5 m in the early 1960s to about 278.1 m in 1983/1984 was the largest to occur within the period of study (1900-2010) and coincides with the combined cold phase of AMO and strong El Niño phase of ENSO. Further analyses show that the current warm phase of AMO and increasing La Niña episodes appear to be playing a major role in the increased precipitation in the Sahel region. The LC level is responding to this increase in precipitation by a gradual recovery, though it is still below the levels of the 1960s. This understanding of the AMO-ENSO-rainfall-LC level association will help in forecasting the impacts of similar combined episodes in the future. These findings also have implications for long-term water resources management in the LC region.

  6. Evaluation of the efficacy of ChAd63-MVA vectored vaccines expressing circumsporozoite protein and ME-TRAP against controlled human malaria infection in malaria-naive individuals.

    PubMed

    Hodgson, Susanne H; Ewer, Katie J; Bliss, Carly M; Edwards, Nick J; Rampling, Thomas; Anagnostou, Nicholas A; de Barra, Eoghan; Havelock, Tom; Bowyer, Georgina; Poulton, Ian D; de Cassan, Simone; Longley, Rhea; Illingworth, Joseph J; Douglas, Alexander D; Mange, Pooja B; Collins, Katharine A; Roberts, Rachel; Gerry, Stephen; Berrie, Eleanor; Moyle, Sarah; Colloca, Stefano; Cortese, Riccardo; Sinden, Robert E; Gilbert, Sarah C; Bejon, Philip; Lawrie, Alison M; Nicosia, Alfredo; Faust, Saul N; Hill, Adrian V S

    2015-04-01

    Circumsporozoite protein (CS) is the antigenic target for RTS,S, the most advanced malaria vaccine to date. Heterologous prime-boost with the viral vectors simian adenovirus 63 (ChAd63)-modified vaccinia virus Ankara (MVA) is the most potent inducer of T-cells in humans, demonstrating significant efficacy when expressing the preerythrocytic antigen insert multiple epitope-thrombospondin-related adhesion protein (ME-TRAP). We hypothesized that ChAd63-MVA containing CS may result in a significant clinical protective efficacy. We conducted an open-label, 2-site, partially randomized Plasmodium falciparum sporozoite controlled human malaria infection (CHMI) study to compare the clinical efficacy of ChAd63-MVA CS with ChAd63-MVA ME-TRAP. One of 15 vaccinees (7%) receiving ChAd63-MVA CS and 2 of 15 (13%) receiving ChAd63-MVA ME-TRAP achieved sterile protection after CHMI. Three of 15 vaccinees (20%) receiving ChAd63-MVA CS and 5 of 15 (33%) receiving ChAd63-MVA ME-TRAP demonstrated a delay in time to treatment, compared with unvaccinated controls. In quantitative polymerase chain reaction analyses, ChAd63-MVA CS was estimated to reduce the liver parasite burden by 69%-79%, compared with 79%-84% for ChAd63-MVA ME-TRAP. ChAd63-MVA CS does reduce the liver parasite burden, but ChAd63-MVA ME-TRAP remains the most promising antigenic insert for a vectored liver-stage vaccine. Detailed analyses of parasite kinetics may allow detection of smaller but biologically important differences in vaccine efficacy that can influence future vaccine development. NCT01623557. © The Author 2014. Published by Oxford University Press on behalf of the Infectious Diseases Society of America.

  7. Lake viruses lyse cyanobacteria, Cylindrospermopsis raciborskii, enhances filamentous-host dispersal in Australia

    NASA Astrophysics Data System (ADS)

    Pollard, Peter C.; Young, Loretta M.

    2010-01-01

    Globally, cyanobacterial blooms are increasing along with observations of the controlling influence of viruses. Our aim here was to test whether viruses from an Australian freshwater lake could lyse the cyanobacterium Cylindrospermopsis raciborskii (Woloszynska) Seenaya and Subba Raju. C. raciborskii was selectively isolated from Lake Samsonvale southeast Queensland Australia using a Modified Jaworski Medium (without any form of inorganic nitrogen). Microscopy confirmed the resulting culture of a single cyanobacterial species. Natural viral-like particles (VLPs) were incubated with C. raciborskii cells, the host abundance decreased by 86% in 5 days, while the number of VLPs increased stepwise. As a cell lysed, the filaments of cells split into smaller, but viable, fragments. This process may help disperse the cyanobacterium in the wild. Hence the use of this virus to control blooms may inadvertently encourage the dispersal of toxic filamentous cyanobacteria. The cyanophage (virus infecting cyanobacteria) replication time was 21 h, with an average burst size of 64 viruses cell -1. Transmission Electron Microscopy showed this cyanophage for C. raciborskii, with its long, non-contractile tail and a capsid diameter of 70 nm, belongs to the Siphoviridae family of viruses. This cyanophage can affect the abundance and distribution of the cyanobacterium C. raciborskii in this Australian freshwater lake.

  8. Spatial analysis from remotely sensed observations of Congo basin of East African high Land to drain water using gravity for sustainable management of low laying Chad basin of Central Africa

    NASA Astrophysics Data System (ADS)

    Modu, B.; Herbert, B.

    2014-11-01

    The Chad basin which covers an area of about 2.4 million kilometer square is one of the largest drainage basins in Africa in the centre of Lake Chad .This basin was formed as a result of rifting and drifting episode, as such it has no outlet to the oceans or seas. It contains large area of desert from the north to the west. The basin covers in part seven countries such as Chad, Nigeria, Central African Republic, Cameroun, Niger, Sudan and Algeria. It is named Chad basin because 43.9% falls in Chad republic. Since its formation, the basin continues to experienced water shortage due to the activities of Dams combination, increase in irrigations and general reduction in rainfall. Chad basin needs an external water source for it to be function at sustainable level, hence needs for exploitation of higher east African river basin called Congo basin; which covers an area of 3.7 million square km lies in an astride the equator in west-central Africa-world second largest river basin after Amazon. The Congo River almost pans around republic of Congo, the democratic republic of Congo, the Central African Republic, western Zambia, northern Angola, part of Cameroun, and Tanzania. The remotely sensed imagery analysis and observation revealed that Congo basin is on the elevation of 275 to 460 meters and the Chad basin is on elevation of 240 meters. This implies that water can be drained from Congo basin via headrace down to the Chad basin for the water sustainability.

  9. Application of CHAD hydrodynamics to shock-wave problems

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Trease, H.E.; O`Rourke, P.J.; Sahota, M.S.

    1997-12-31

    CHAD is the latest in a sequence of continually evolving computer codes written to effectively utilize massively parallel computer architectures and the latest grid generators for unstructured meshes. Its applications range from automotive design issues such as in-cylinder and manifold flows of internal combustion engines, vehicle aerodynamics, underhood cooling and passenger compartment heating, ventilation, and air conditioning to shock hydrodynamics and materials modeling. CHAD solves the full unsteady Navier-Stoke equations with the k-epsilon turbulence model in three space dimensions. The code has four major features that distinguish it from the earlier KIVA code, also developed at Los Alamos. First, itmore » is based on a node-centered, finite-volume method in which, like finite element methods, all fluid variables are located at computational nodes. The computational mesh efficiently and accurately handles all element shapes ranging from tetrahedra to hexahedra. Second, it is written in standard Fortran 90 and relies on automatic domain decomposition and a universal communication library written in standard C and MPI for unstructured grids to effectively exploit distributed-memory parallel architectures. Thus the code is fully portable to a variety of computing platforms such as uniprocessor workstations, symmetric multiprocessors, clusters of workstations, and massively parallel platforms. Third, CHAD utilizes a variable explicit/implicit upwind method for convection that improves computational efficiency in flows that have large velocity Courant number variations due to velocity of mesh size variations. Fourth, CHAD is designed to also simulate shock hydrodynamics involving multimaterial anisotropic behavior under high shear. The authors will discuss CHAD capabilities and show several sample calculations showing the strengths and weaknesses of CHAD.« less

  10. Wind-driven Water Bodies : a new paradigm for lake geology

    NASA Astrophysics Data System (ADS)

    Nutz, A.; Schuster, M.; Ghienne, J. F.; Roquin, C.; Bouchette, F. A.

    2015-12-01

    In this contribution we emphasize the importance in some lakes of wind-related hydrodynamic processes (fair weather waves, storm waves, and longshore, cross-shore and bottom currents) as a first order forcing for clastics remobilization and basin infill. This alternative view contrasts with more classical depositional models for lakes where fluvial-driven sedimentation and settling dominates. Here we consider three large lakes/paleo-lakes that are located in different climatic and geodynamic settings: Megalake Chad (north-central Africa), Lake Saint-Jean (Québec, Canada), and Lake Turkana (Kenya, East African Rift System). All of these three lake systems exhibit well developed modern and ancient high-energy littoral morphosedimentary structures which directly derive from wind-related hydrodynamics. The extensive paleo-shorelines of Megalake Chad are composed of beach-foredune ridges, spits, wave-dominated deltas, barriers, and wave-ravinment surface. For Lake Saint-Jean the influence of wind is also identified below the wave-base at lake bottom from erosional surfaces, and sediment drifts. In the Lake Turkana Basin, littoral landforms and deposits are identified for three different time intervals (today, Holocene, Plio-Pleistocene) evidencing that wind-driven hydrodynamics can be preserved in the geological record. Moreover, a preliminary global survey suggests that numerous modern lakes (remote sensing) and paleo-lakes (bibliographic review) behave as such. We thus coin the term "Wind-driven Water Bodies" (WWB) to refer to those lake systems where sedimentation (erosion, transport, deposition) is dominated by wind-induced hydrodynamics at any depth, as it is the case in the marine realm for shallow seas. Integrating wind forcing in lake models has strong implications for basin analysis (paleoenvironments and paleoclimates restitutions, resources exploration), but also for coastal engineering, wildlife and reservoirs management, or leisure activities.

  11. Random demographic household surveys in highly mobile pastoral communities in Chad

    PubMed Central

    Béchir, Mahamat; Hattendorf, Jan; Bonfoh, Bassirou; Zinsstag, Jakob; Schelling, Esther

    2011-01-01

    Abstract Problem Reliable demographic data is a central requirement for health planning and management, and for the implementation of adequate interventions. This study addresses the lack of demographic data on mobile pastoral communities in the Sahel. Approach A total of 1081 Arab, Fulani and Gorane women and 2541 children (1336 boys and 1205 girls) were interviewed and registered by a biometric fingerprint scanner in five repeated random transect demographic and health surveys conducted from March 2007 to January 2008 in the Lake Chad region in Chad. Local setting Important determinants for the planning and implementation of household surveys among mobile pastoral communities include: environmental factors; availability of women for interviews; difficulties in defining “own” children; the need for information-education-communication campaigns; and informed consent of husbands in typically patriarchal societies. Relevant changes Due to their high mobility, only 5% (56/1081) of registered women were encountered twice. Therefore, it was not possible to establish a demographic and health cohort. Lessons learnt Prospective demographic and health cohorts are the most accurate method to assess child mortality and other demographic indices. However, their feasibility in a highly mobile pastoral setting remains to be shown. Future interdisciplinary scientific efforts need to target innovative methods, tools and approaches to include marginalized communities in operational health and demographic surveillance systems. PMID:21556307

  12. Seroprevalence of Rift Valley fever, Q fever, and brucellosis in ruminants on the southeastern shore of Lake Chad.

    PubMed

    Abakar, Mahamat Fayiz; Naré, Ngandolo B; Schelling, Esther; Hattendorf, Jan; Alfaroukh, Idriss O; Zinsstag, Jakob

    2014-10-01

    The seroprevalence of Rift Valley fever (RVF), brucellosis, and Q fever among domestic ruminants on the southeastern shore of Lake Chad was studied. The study area consisted of two parts, including mainland and islands. On the mainland, the study was conducted in nine randomly selected villages and camps. On the islands, samples were collected from all four available sites. A total of 985 serum samples were collected and 924 were analyzed using enzyme-linked immunosorbent assay (ELISA) for RVF. A total of 561 samples collected from islands were analyzed using ELISA for Q fever and both ELISA and Rose Bengal tests (RBT) for brucellosis. The apparent RVF seroprevalence by species was 37.8% (95% confidence interval [CI] 34.2-41.3) in cattle, 18.8% (95% CI 12.3-25.2) in goats, and 10.8% (95% CI 3.0-18.5) in sheep. For brucellosis and Q fever, only cattle samples from islands were analyzed. For Q fever, the apparent seroprevalence was 7.8% (95% CI 5.6-10.1). For brucellosis, the RBT showed a prevalence of 5.7% (95% CI 3.8-7.6), and ELISA showed 11.9% (95% CI 9.3-14.6) with a kappa value of 0.53 showing a moderate agreement between the two tests. This study confirms the presence of the three diseases in the study area. More research is required to assess the importance for public health and conservation of the Kouri cattle breed.

  13. Influence of the surface permeability on the GRACE water mass variations. Case of the Lake Chad basin.

    NASA Astrophysics Data System (ADS)

    Lopez, T.; Ramillien, G.; Antoine, R.; Darrozes, J.; Rabinowicz, M.

    2017-12-01

    Since its launch in 2002, the Gravity Recovery And Climate Experiment (GRACE) has been measured the tiny variations of the gravity field due to redistributions of water mass in the surface envelops of Earth. At a spatial resolution of 400 km, these satellite data offer a unique perspective to understand the evolution of continental water storage at regional and global scales, and therefore they enable the monitoring of the hydrological systems such as river basins. It is well known that seasonal cycle, droughts, vegetation and human extractions are the main contributors of the hydrology signals sensed by GRACE. However, the coupling between land surface and the atmosphere is important in semi-arid and arid regions, in particular in West Africa [1]. We propose to quantify the surface water fluxes in the Lake Chad region by using the 10-day water mass solutions of the GRACE mission in the context of the regular West African monsoon. Alternation of the evaporation/condensation cycles during the recent period are interpreted in terms of surface vertical permeability changes that control the thermal evolution in this region [2]. GRACE solutions reveal an interannual increase of surface water mass at the beginning of the dry seasons, especially between 2005 and 2008. We propose that this gain of surface water mass is caused by a seasonal cycle of clay fracturing that controls the evaporation/condensation cycle. [1] Koster et al. (2004). Science, 305, 1138-1140. [2] Lopez et al. (2016). Surv. Geophys., 37 (2), 471-502.

  14. The Consolidated Human Activity Database — Master Version (CHAD-Master) Technical Memorandum

    EPA Pesticide Factsheets

    This technical memorandum contains information about the Consolidated Human Activity Database -- Master version, including CHAD contents, inventory of variables: Questionnaire files and Event files, CHAD codes, and references.

  15. Safety and immunogenicity of heterologous prime-boost immunisation with Plasmodium falciparum malaria candidate vaccines, ChAd63 ME-TRAP and MVA ME-TRAP, in healthy Gambian and Kenyan adults.

    PubMed

    Ogwang, Caroline; Afolabi, Muhammed; Kimani, Domtila; Jagne, Ya Jankey; Sheehy, Susanne H; Bliss, Carly M; Duncan, Christopher J A; Collins, Katharine A; Garcia Knight, Miguel A; Kimani, Eva; Anagnostou, Nicholas A; Berrie, Eleanor; Moyle, Sarah; Gilbert, Sarah C; Spencer, Alexandra J; Soipei, Peninah; Mueller, Jenny; Okebe, Joseph; Colloca, Stefano; Cortese, Riccardo; Viebig, Nicola K; Roberts, Rachel; Gantlett, Katherine; Lawrie, Alison M; Nicosia, Alfredo; Imoukhuede, Egeruan B; Bejon, Philip; Urban, Britta C; Flanagan, Katie L; Ewer, Katie J; Chilengi, Roma; Hill, Adrian V S; Bojang, Kalifa

    2013-01-01

    Heterologous prime boost immunization with chimpanzee adenovirus 63 (ChAd63) and Modified vaccinia Virus Ankara (MVA) vectored vaccines is a strategy recently shown to be capable of inducing strong cell mediated responses against several antigens from the malaria parasite. ChAd63-MVA expressing the Plasmodium falciparum pre-erythrocytic antigen ME-TRAP (multiple epitope string with thrombospondin-related adhesion protein) is a leading malaria vaccine candidate, capable of inducing sterile protection in malaria naïve adults following controlled human malaria infection (CHMI). We conducted two Phase Ib dose escalation clinical trials assessing the safety and immunogenicity of ChAd63-MVA ME-TRAP in 46 healthy malaria exposed adults in two African countries with similar malaria transmission patterns. ChAd63-MVA ME-TRAP was shown to be safe and immunogenic, inducing high-level T cell responses (median >1300 SFU/million PBMC). ChAd63-MVA ME-TRAP is a safe and highly immunogenic vaccine regimen in adults with prior exposure to malaria. Further clinical trials to assess safety and immunogenicity in children and infants and protective efficacy in the field are now warranted. Pactr.org PACTR2010020001771828 Pactr.org PACTR201008000221638 ClinicalTrials.gov NCT01373879 NCT01373879 ClinicalTrials.gov NCT01379430 NCT01379430.

  16. Observed climate variability over Chad using multiple observational and reanalysis datasets

    NASA Astrophysics Data System (ADS)

    Maharana, Pyarimohan; Abdel-Lathif, Ahmat Younous; Pattnayak, Kanhu Charan

    2018-03-01

    Chad is the largest of Africa's landlocked countries and one of the least studied region of the African continent. The major portion of Chad lies in the Sahel region, which is known for its rapid climate change. In this study, multiple observational datasets are analyzed from 1950 to 2014, in order to examine the trend of precipitation and temperature along with their variability over Chad to understand possible impacts of climate change over this region. Trend analysis of the climatic fields has been carried out using Mann-Kendall test. The precipitation over Chad is mostly contributed during summer by West African Monsoon, with maximum northward limit of 18° N. The Atlantic Ocean as well as the Mediterranean Sea are the major source of moisture for the summer rainfall over Chad. Based on the rainfall time series, the entire study period has been divided in to wet (1950 to 1965), dry (1966 to 1990) and recovery period (1991 to 2014). The rainfall has decreased drastically for almost 3 decades during the dry period resulted into various drought years. The temperature increases at a rate of 0.15 °C/decade during the entire period of analysis. The seasonal rainfall as well as temperature plays a major role in the change of land use/cover. The decrease of monsoon rainfall during the dry period reduces the C4 cover drastically; this reduction of C4 grass cover leads to increase of C3 grass cover. The slow revival of rainfall is still not good enough for the increase of shrub cover but it favors the gradual reduction of bare land over Chad.

  17. Progress toward poliomyelitis eradication--Chad, January 2011-August 2012.

    PubMed

    2012-10-26

    In 1988, the World Health Assembly launched the Global Polio Eradication Initiative (GPEI) to interrupt transmission of wild poliovirus (WPV). By January 2012, indigenous WPV transmission had been interrupted in all countries except Afghanistan, Pakistan, and Nigeria. However, importation of WPV caused outbreaks in 29 and reestablished transmission in four, previously polio-free African countries during 2003-2011. Transmission after WPV importation is considered reestablished when it continues for ≥ 12 months; in Chad, transmissions of WPV type 3 (WPV3) and WPV type 1 (WPV1) were reestablished. WPV3 was imported from Nigeria in 2007 and continued to circulate; the latest reported WPV3 case occurred on March 10, 2011. Transmission of WPV1 continued after a WPV1 case was imported from Nigeria in September 2010; the latest reported WPV1 occurred on June 14, 2012. This report updates previous reports and describes polio eradication activities and progress in Chad during January 2011-August 2012, as of October 2, 2012. Five WPV1 cases were reported during January-August 2012, compared with 111 WPV1 cases and three WPV3 cases reported during the same period in 2011. Five circulating type 2 vaccine-derived poliovirus (cVDPV2) cases occurred during July-August 2012. Current progress suggests that Chad could interrupt reestablished WPV transmission in 2012, although limitations in surveillance hamper the ability to detect ongoing transmission. Furthermore, with ongoing endemic WPV transmission in Nigeria, Chad remains at risk for new WPV importations. Efforts to strengthen surveillance and enhance routine and campaign immunization performance will need to continue in Chad to ensure interruption of reestablished WPV transmission, limit circulation after any WPV importation, and interrupt transmission of cVDPV.

  18. Safety and Immunogenicity of Heterologous Prime-Boost Immunisation with Plasmodium falciparum Malaria Candidate Vaccines, ChAd63 ME-TRAP and MVA ME-TRAP, in Healthy Gambian and Kenyan Adults

    PubMed Central

    Kimani, Domtila; Jagne, Ya Jankey; Sheehy, Susanne H.; Bliss, Carly M.; Duncan, Christopher J. A.; Collins, Katharine A.; Garcia Knight, Miguel A.; Kimani, Eva; Anagnostou, Nicholas A.; Berrie, Eleanor; Moyle, Sarah; Gilbert, Sarah C.; Spencer, Alexandra J.; Soipei, Peninah; Mueller, Jenny; Okebe, Joseph; Colloca, Stefano; Cortese, Riccardo; Viebig, Nicola K.; Roberts, Rachel; Gantlett, Katherine; Lawrie, Alison M.; Nicosia, Alfredo; Imoukhuede, Egeruan B.; Bejon, Philip; Urban, Britta C.; Flanagan, Katie L.; Ewer, Katie J.; Chilengi, Roma; Hill, Adrian V. S.; Bojang, Kalifa

    2013-01-01

    Background Heterologous prime boost immunization with chimpanzee adenovirus 63 (ChAd63) and Modified vaccinia Virus Ankara (MVA) vectored vaccines is a strategy recently shown to be capable of inducing strong cell mediated responses against several antigens from the malaria parasite. ChAd63-MVA expressing the Plasmodium falciparum pre-erythrocytic antigen ME-TRAP (multiple epitope string with thrombospondin-related adhesion protein) is a leading malaria vaccine candidate, capable of inducing sterile protection in malaria naïve adults following controlled human malaria infection (CHMI). Methodology We conducted two Phase Ib dose escalation clinical trials assessing the safety and immunogenicity of ChAd63-MVA ME-TRAP in 46 healthy malaria exposed adults in two African countries with similar malaria transmission patterns. Results ChAd63-MVA ME-TRAP was shown to be safe and immunogenic, inducing high-level T cell responses (median >1300 SFU/million PBMC). Conclusions ChAd63-MVA ME-TRAP is a safe and highly immunogenic vaccine regimen in adults with prior exposure to malaria. Further clinical trials to assess safety and immunogenicity in children and infants and protective efficacy in the field are now warranted. Trial Registration Pactr.org PACTR2010020001771828 Pactr.org PACTR201008000221638 ClinicalTrials.gov NCT01373879 NCT01373879 ClinicalTrials.gov NCT01379430 NCT01379430 PMID:23526949

  19. Honey Bee Infecting Lake Sinai Viruses.

    PubMed

    Daughenbaugh, Katie F; Martin, Madison; Brutscher, Laura M; Cavigli, Ian; Garcia, Emma; Lavin, Matt; Flenniken, Michelle L

    2015-06-23

    Honey bees are critical pollinators of important agricultural crops. Recently, high annual losses of honey bee colonies have prompted further investigation of honey bee infecting viruses. To better characterize the recently discovered and very prevalent Lake Sinai virus (LSV) group, we sequenced currently circulating LSVs, performed phylogenetic analysis, and obtained images of LSV2. Sequence analysis resulted in extension of the LSV1 and LSV2 genomes, the first detection of LSV4 in the US, and the discovery of LSV6 and LSV7. We detected LSV1 and LSV2 in the Varroa destructor mite, and determined that a large proportion of LSV2 is found in the honey bee gut, suggesting that vector-mediated, food-associated, and/or fecal-oral routes may be important for LSV dissemination. Pathogen-specific quantitative PCR data, obtained from samples collected during a small-scale monitoring project, revealed that LSV2, LSV1, Black queen cell virus (BQCV), and Nosema ceranae were more abundant in weak colonies than strong colonies within this sample cohort. Together, these results enhance our current understanding of LSVs and illustrate the importance of future studies aimed at investigating the role of LSVs and other pathogens on honey bee health at both the individual and colony levels.

  20. Honey Bee Infecting Lake Sinai Viruses

    PubMed Central

    Daughenbaugh, Katie F.; Martin, Madison; Brutscher, Laura M.; Cavigli, Ian; Garcia, Emma; Lavin, Matt; Flenniken, Michelle L.

    2015-01-01

    Honey bees are critical pollinators of important agricultural crops. Recently, high annual losses of honey bee colonies have prompted further investigation of honey bee infecting viruses. To better characterize the recently discovered and very prevalent Lake Sinai virus (LSV) group, we sequenced currently circulating LSVs, performed phylogenetic analysis, and obtained images of LSV2. Sequence analysis resulted in extension of the LSV1 and LSV2 genomes, the first detection of LSV4 in the US, and the discovery of LSV6 and LSV7. We detected LSV1 and LSV2 in the Varroa destructor mite, and determined that a large proportion of LSV2 is found in the honey bee gut, suggesting that vector-mediated, food-associated, and/or fecal-oral routes may be important for LSV dissemination. Pathogen-specific quantitative PCR data, obtained from samples collected during a small-scale monitoring project, revealed that LSV2, LSV1, Black queen cell virus (BQCV), and Nosema ceranae were more abundant in weak colonies than strong colonies within this sample cohort. Together, these results enhance our current understanding of LSVs and illustrate the importance of future studies aimed at investigating the role of LSVs and other pathogens on honey bee health at both the individual and colony levels. PMID:26110586

  1. Situational analysis of infant and young child nutrition policies and programmatic activities in Chad.

    PubMed

    Wuehler, Sara E; Nadjilem, Djasndibye

    2011-04-01

    Progress towards reducing mortality and malnutrition among children <5 years of age has been less than needed to achieve related Millennium Development Goals (MDGs). Therefore, several international agencies joined to 'reposition children's right to adequate nutrition in the Sahel', starting with an analysis of current activities related to infant and young child nutrition (IYCN). The main objectives of the situational analysis are to compile, analyse, and interpret available information on infant and child feeding, and the nutrition situation of children <2 years of age in Chad, as one of the six targeted countries. These findings are available to assist in identifying inconsistencies and filling gaps in current programming. Between June and October of 2008, key informants responsible for IYCN-related activities in Chad were interviewed, and 53 documents were examined on the following themes: the promotion of optimal breastfeeding and complementary feeding practices, prevention of micronutrient deficiencies, management of acute malnutrition, prevention of mother-to-child transmission of human immunodeficiency virus (HIV), food security, and promotion of good hygienic practices. Chad is not on track to reaching the MDGs of reducing mortality by two-thirds and malnutrition by half among children <5 years of age between 1990 and 2015. Most of the key IYCN topics were addressed in a national policy to combat malnutrition and micronutrient deficiencies. No national nutrition policy was yet ratified in Chad, so the target of many documents reviewed was the malnourished child. Researchers have identified some barriers to optimal feeding practices. However, the majority of these surveys were small scale, so they do not necessarily provide information relevant to the general population. Expanded surveys would be needed for developing evidence-based educational messages targeted to local needs. Reviewed training materials and related programmes being implemented in Chad

  2. New Insights into the Lake Chad Basin Population Structure Revealed by High-Throughput Genotyping of Mitochondrial DNA Coding SNPs

    PubMed Central

    Černý, Viktor; Carracedo, Ángel

    2011-01-01

    Background Located in the Sudan belt, the Chad Basin forms a remarkable ecosystem, where several unique agricultural and pastoral techniques have been developed. Both from an archaeological and a genetic point of view, this region has been interpreted to be the center of a bidirectional corridor connecting West and East Africa, as well as a meeting point for populations coming from North Africa through the Saharan desert. Methodology/Principal Findings Samples from twelve ethnic groups from the Chad Basin (n = 542) have been high-throughput genotyped for 230 coding region mitochondrial DNA (mtDNA) Single Nucleotide Polymorphisms (mtSNPs) using Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight (MALDI-TOF) mass spectrometry. This set of mtSNPs allowed for much better phylogenetic resolution than previous studies of this geographic region, enabling new insights into its population history. Notable haplogroup (hg) heterogeneity has been observed in the Chad Basin mirroring the different demographic histories of these ethnic groups. As estimated using a Bayesian framework, nomadic populations showed negative growth which was not always correlated to their estimated effective population sizes. Nomads also showed lower diversity values than sedentary groups. Conclusions/Significance Compared to sedentary population, nomads showed signals of stronger genetic drift occurring in their ancestral populations. These populations, however, retained more haplotype diversity in their hypervariable segments I (HVS-I), but not their mtSNPs, suggesting a more ancestral ethnogenesis. Whereas the nomadic population showed a higher Mediterranean influence signaled mainly by sub-lineages of M1, R0, U6, and U5, the other populations showed a more consistent sub-Saharan pattern. Although lifestyle may have an influence on diversity patterns and hg composition, analysis of molecular variance has not identified these differences. The present study indicates that analysis of mt

  3. [Taxonomic status of the Tyulek virus (TLKV) (Orthomyxoviridae, Quaranjavirus, Quaranfil group) isolated from the ticks Argas vulgaris Filippova, 1961 (Argasidae) from the birds burrow nest biotopes in the Kyrgyzstan].

    PubMed

    L'vov, D K; Al'khovskiĭ, S V; Shchelkanov, M Iu; Shchetinin, A M; Deriabin, P G; Aristova, V A; Gitel'man, A K; Samokhvalov, E I; Botikov, A G

    2014-01-01

    The Tyulek virus (TLKV) was isolated from the ticks Argas vulgaris Filippova, 1961 (Argasidae), collected from the burrow biotopes in multispecies birds colony in the Aksu river floodplain near Tyulek village (northern part of Chu Valley, Kyrgyzstan). Recently, the TLKV was assigned to the Quaranfil group (including the Quaranfil virus (QRFV), Johnston Atoll virus (JAV), Lake Chad virus) that is a novel genus of the Quaranjavirus in the Orthomyxoviridae family. In his work, the complete genome (ID GenBank KJ438647-8) sequence of the TLKV was determined using next-generation sequencing (Illumina platform). Comparison of deduced amino acid sequences shows closed relationship of the TLKV with QRFV and JAV (86% and 84% identity for PB1 and about 70% for PB2 and PA, respectively). The identity level of the TLKV and QRFV in outer glycoprotein GP is 72% and 80% for nucleotide and amino acid sequences, respectively. The phylogenetic analysis showed that the TLKV belongs to the genus of the Quaranjavirus in the family Orthomyxoviridae.

  4. A climate trend analysis of Chad

    USGS Publications Warehouse

    Funk, Christopher C.; Rowland, Jim; Adoum, Alkhalil; Eilerts, Gary; White, Libby

    2012-01-01

    This brief report, drawing from a multi-year effort by the U.S. Agency for International Development (USAID) Famine Early Warning Systems Network (FEWS NET), identifies significant decreases in rainfall and increases in air temperature across Chad, especially in the eastern part of the country. These analyses are based on quality-controlled station observations. Conclusions:* Summer rains have decreased in eastern Chad during the past 20 years. * Temperatures have increased by 0.8 °Celsius since 1975, amplifying the effect of droughts. * Crop yields are very low and stagnant. * The amount of farmland per person is low, and decliningrapidly.* Population growth combined with stagnating yieldscould lead to a 30 percent reduction in per capita cereal production by 2025.* In many cases, areas with changing climate are coincident with zones of substantial conflict, indicating some degree of association; however, the contribution of climate change to these conflicts is not currently understood.

  5. Analysis of seasonal characteristics of Sambhar Salt Lake, India, from digitized Space Shuttle photography

    NASA Technical Reports Server (NTRS)

    Lulla, Kamlesh P.; Helfert, Michael R.

    1989-01-01

    Sambhar Salt Lake is the largest salt lake (230 sq km) in India, situated in the northwest near Jaipur. Analysis of Space Shuttle photographs of this ephemeral lake reveals that water levels and lake basin land-use information can be extracted by both the digital and manual analysis techniques. Seasonal characteristics captured by the two Shuttle photos used in this study show that additional land use/cover categories can be mapped from the dry season photos. This additional information is essential for precise cartographic updates, and provides seasonal hydrologic profiles and inputs for potential mesoscale climate modeling. This paper extends the digitization and mensuration techniques originally developed for space photography and applied to other regions (e.g., Lake Chad, Africa, and Great Salt Lake, USA).

  6. Diversity of virus-host systems in hypersaline Lake Retba, Senegal.

    PubMed

    Sime-Ngando, Télesphore; Lucas, Soizick; Robin, Agnès; Tucker, Kimberly Pause; Colombet, Jonathan; Bettarel, Yvan; Desmond, Elie; Gribaldo, Simonetta; Forterre, Patrick; Breitbart, Mya; Prangishvili, David

    2011-08-01

    Remarkable morphological diversity of virus-like particles was observed by transmission electron microscopy in a hypersaline water sample from Lake Retba, Senegal. The majority of particles morphologically resembled hyperthermophilic archaeal DNA viruses isolated from extreme geothermal environments. Some hypersaline viral morphotypes have not been previously observed in nature, and less than 1% of observed particles had a head-and-tail morphology, which is typical for bacterial DNA viruses. Culture-independent analysis of the microbial diversity in the sample suggested the dominance of extremely halophilic archaea. Few of the 16S sequences corresponded to known archeal genera (Haloquadratum, Halorubrum and Natronomonas), whereas the majority represented novel archaeal clades. Three sequences corresponded to a new basal lineage of the haloarchaea. Bacteria belonged to four major phyla, consistent with the known diversity in saline environments. Metagenomic sequencing of DNA from the purified virus-like particles revealed very few similarities to the NCBI non-redundant database at either the nucleotide or amino acid level. Some of the identifiable virus sequences were most similar to previously described haloarchaeal viruses, but no sequence similarities were found to archaeal viruses from extreme geothermal environments. A large proportion of the sequences had similarity to previously sequenced viral metagenomes from solar salterns. © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd.

  7. Highly Pathogenic Avian Influenza A(H5N8) Virus in Wild Migratory Birds, Qinghai Lake, China.

    PubMed

    Li, Mingxin; Liu, Haizhou; Bi, Yuhai; Sun, Jianqing; Wong, Gary; Liu, Di; Li, Laixing; Liu, Juxiang; Chen, Quanjiao; Wang, Hanzhong; He, Yubang; Shi, Weifeng; Gao, George F; Chen, Jianjun

    2017-04-01

    In May 2016, a highly pathogenic avian influenza A(H5N8) virus strain caused deaths among 3 species of wild migratory birds in Qinghai Lake, China. Genetic analysis showed that the novel reassortant virus belongs to group B H5N8 viruses and that the reassortment events likely occurred in early 2016.

  8. Diverse circular replication-associated protein encoding viruses circulating in invertebrates within a lake ecosystem.

    PubMed

    Dayaram, Anisha; Galatowitsch, Mark L; Argüello-Astorga, Gerardo R; van Bysterveldt, Katherine; Kraberger, Simona; Stainton, Daisy; Harding, Jon S; Roumagnac, Philippe; Martin, Darren P; Lefeuvre, Pierre; Varsani, Arvind

    2016-04-01

    Over the last five years next-generation sequencing has become a cost effective and efficient method for identifying known and unknown microorganisms. Access to this technique has dramatically changed the field of virology, enabling a wide range of environmental viral metagenome studies to be undertaken of organisms and environmental samples from polar to tropical regions. These studies have led to the discovery of hundreds of highly divergent single stranded DNA (ssDNA) virus-like sequences encoding replication-associated proteins. Yet, few studies have explored how viruses might be shared in an ecosystem through feeding relationships. Here we identify 169 circular molecules (160 CRESS DNA molecules, nine circular molecules) recovered from a New Zealand freshwater lake, that we have tentatively classified into 51 putatively novel species and five previously described species (DflaCV-3, -5, -6, -8, -10). The CRESS DNA viruses identified in this study were recovered from molluscs (Echyridella menzeisii, Musculium novaezelandiae, Potamopyrgus antipodarum and Physella acuta) and insect larvae (Procordulia grayi, Xanthocnemis zealandica, and Chironomus zealandicus) collected from Lake Sarah, as well as from the lake water and benthic sediments. Extensive diversity was observed across most CRESS DNA molecules recovered. The putative capsid protein of one viral species was found to be most similar to those of members of the Tombusviridae family, thus expanding the number of known RNA-DNA hybrid viruses in nature. We noted a strong association between the CRESS DNA viruses and circular molecules identified in the water and browser organisms (C. zealandicus, P. antipodarum and P. acuta), and between water sediments and undefended prey species (C. zealandicus). However, we were unable to find any significant correlation of viral assemblages to the potential feeding relationships of the host aquatic invertebrates. Copyright © 2016 Elsevier B.V. All rights reserved.

  9. [History of the public health policy in Chad 1900-1995].

    PubMed

    Massenet, D

    1996-01-01

    Public health policy in Chad began after colonization in 1899 and remained under the control of French Army Medical Corps for a long time. Military doctors shared their time between treating service personnel and indigenous people entitled Medical Assistance and making rounds in their sector. Since independence public health in the country has been based on a two-pronged association including fixed facilities (hospitals and dispensaries) and mobile services such as the Endemic Disease Unit whose most notable success was control of sleeping sickness in the southern part of the country. Over the years Chad has built up a national medical staff comprising 150 physicians. A medical school was opened in N'Djamena in 1990 and paramedical personnel are now trained at the National School for Public Health. War and lack of funds interrupted mobile services and there is presently a recrudescence of sleeping sickness. Since 1990 the World Health Organization has imposed its views and primary care is now available for all. However, it is now too early to judge the efficacity of this program in Chad.

  10. Water levels and artesian pressures in the Chad Basin of northeastern Nigeria, 1963-68

    USGS Publications Warehouse

    Carmalt, S.W.; Tibbitts, G.C.

    1969-01-01

    This report presents records of water levels and artesian pressures collected during 1963-68 on an observational network of 116 dug wells and boreholes (drilled wells) in the Chad Basin of northeastern Nigeria. The Chad Basin is underlain by the Chad Formation, a series of fluvio-lacustrine sediments which attain a thickness of 1,500 feet or more in Nigeria. Three water-bearing zones, designated Upper, Middle and Lower, have been identified in the Chad Formation of Nigeria. The Upper Zone aquifer, which contains water under both unconfined and confined conditions, provides the principal source of water to dug wells for domestic and village water supply. The Middle Zone aquifer is tapped by numerous deep boreholes (drilled wells) which provide water by artesian flow in more than 13,000 square miles of Nigeria north and east of Maiduguri. The Lower Zone, which is also confined has only been identified thus far (1969) in the vicinity of Maiduguri.

  11. Simplified prediction of postoperative cardiac surgery outcomes with a novel score: R2CHADS2.

    PubMed

    Peguero, Julio G; Lo Presti, Saberio; Issa, Omar; Podesta, Carlos; Parise, Helen; Layka, Ayman; Brenes, Juan C; Lamelas, Joseph; Lamas, Gervasio A

    2016-07-01

    To compare the accuracy of R2CHADS2, CHADS2, and CHA2DS2-VASc scores vs the Society of Thoracic Surgeons (STS) score as predictors of morbidity and mortality after cardiovascular surgery. All patients who underwent cardiothoracic surgery at our institution from January 2008 to July 2013 were analyzed. Only those patients who fulfilled the criteria for STS score calculation were included. The R2CHADS2 score was computed as follows: 2 points for GFR < 60 mL/min/1.73 m(2) (R2), prior stroke or TIA (S2); 1 point for history of congestive heart failure (C), hypertension (H), age ≥75 years (A), or diabetes (D). Area under the curve (AUC) analysis was used to estimate the accuracy of the different scores. The end point variables included operative mortality, permanent stroke, and renal failure as defined by the STS database system. Of the 3,492 patients screened, 2,263 met the inclusion criteria. These included 1,160 (51%) isolated valve surgery, 859 (38%) coronary artery bypass graft surgery, and 245 (11%) combined procedures. There were 147 postoperative events: 75 (3%) patients had postoperative renal failure, 48 (2%) had operative mortality, and 24 (1%) had permanent stroke. AUC analysis revealed that STS, R2CHADS2, CHADS2, and CHA2DS2-VASc reliably estimated all postoperative outcomes. STS and R2CHADS2 scores had the best accuracy overall, with no significant difference in AUC values between them. The R2CHADS2 score estimates postoperative events with acceptable accuracy and if further validated may be used as a simple preoperative risk tool calculator. Copyright © 2016 Elsevier Inc. All rights reserved.

  12. Effects of Land Cover / Land Use, Soil Texture, and Vegetation on the Water Balance of Lake Chad Basin

    NASA Astrophysics Data System (ADS)

    Babamaaji, R. A.; Lee, J.

    2013-12-01

    Lake Chad Basin (LCB) has experienced drastic changes of land cover and poor water management practices during the last 50 years. The successive droughts in the 1970s and 1980s resulted in the shortage of surface water and groundwater resources. This problem of drought has a devastating implication on the natural resources of the Basin with great consequence on food security, poverty reduction and quality of life of the inhabitants in the LCB. Therefore, understanding the effects of land use / land cover must be a first step to find how they disturb cycle especially the groundwater in the LCB. The abundance of groundwater is affected by the climate change through the interaction with surface water, such as lakes and rivers, and disuse recharge through an infiltration process. Quantifying the impact of climate change on the groundwater resource requires reliable forecasting of changes in the major climatic variables and other spatial variations including the land use/land cover, soil texture, topographic slope, and vegetation. In this study, we employed a spatially distributed water balance model WetSpass to simulate a long-term average change of groundwater recharge in the LCB of Africa. WetSpass is a water balance-based model to estimate seasonal and spatial distribution of surface runoff, interception, evapotranspiration, and groundwater recharge. The model is especially suitable for studying the effect of land use/land cover change on the water regime in the LCB. The present study describes the concept of the model and its application to the development of recharge map of the LCB. The study shows that major role in the water balance of LCB. The mean yearly actual evapotranspiration (ET) from the basin range from 60mm - 400 mm, which is 90 % (69mm - 430) of the annual precipitation from 2003 - 2010. It is striking that about 50 - 60 % of the total runoff is produced on build-up (impervious surfaces), while much smaller contributions are obtained from vegetated

  13. Space Radar of Image Aorounga Impact Crater, Chad

    NASA Image and Video Library

    1999-04-15

    The impact of an asteroid or comet several hundred million years ago left scars in the landscape that are still visible in this spaceborne radar image of an area in the Sahara Desert of northern Chad.

  14. [Evaluation of the lifetime of nail markings during polio vaccinations in Chad].

    PubMed

    Quoc Cuong, Huong; Schlumberger, Martin; Garba Tchang, Salomon; Ould Cheikh, Dah; Savès, Marianne; Mallah, Barah; Demtilo Attilo, Jacques; Ngangro Mosurel, Ndeikoundam; Gamatié, Youssouf

    2010-01-01

    SID (Supplemental Immunization Days) is a special strategy intended to accelerate eradication of poliomyelitis in countries where it is still endemic (India, Afghanistan, and Pakistan in Asia, and Nigeria in Africa). This strategy is also applied in Nigeria's neighbours (Cameroon, Chad, Niger and Benin). Since the poliomyelitis virus was imported from Nigeria in 2001, Chad has reported cases of poliomyelitis every year. After 30 SIDs in Chad and the inaccurate or false attribution of side-effects to polio vaccines, some groups persistently refuse polio vaccination. To ascertain the true coverage of SID, the Ministry of Health and several partners (WHO, UNICEF and Rotary) conduct external coverage evaluations, to identify the under-vaccinated areas where population may be refusing immunization. The nails of the children receiving vaccinations are marked with indelible ink and those markings are the best indicator of the area's actual SID coverage. When coverage investigators arrive and propose vaccination to all children not immunized during SID, mothers who wish to refuse vaccination may claim that the children's markings disappeared after a few days, due to bathing. WHO experts have found that markings applied to their own nails with the WHO-recommended markers persist a few weeks, but others suggested that the markings may disappear much faster among children living in a traditional tropical environment. Until now, the lifetime of these markings has not been tested among children in Africa. To determine the lifetime of the fingernail markings after SID and factors that influence this lifetime in children young than 5 years old in Chad. This prospective cohort study of 200 children (aged 0 to 59 months) took place from March to May 2009 in Milezi, a health zone north of Ndjamena, the capital of Chad, in central Africa. These children received nail markings on their left little finger with an indelible marker pen provided by WHO. The finger was monitored for 35

  15. Chad Genetic Diversity Reveals an African History Marked by Multiple Holocene Eurasian Migrations.

    PubMed

    Haber, Marc; Mezzavilla, Massimo; Bergström, Anders; Prado-Martinez, Javier; Hallast, Pille; Saif-Ali, Riyadh; Al-Habori, Molham; Dedoussis, George; Zeggini, Eleftheria; Blue-Smith, Jason; Wells, R Spencer; Xue, Yali; Zalloua, Pierre A; Tyler-Smith, Chris

    2016-12-01

    Understanding human genetic diversity in Africa is important for interpreting the evolution of all humans, yet vast regions in Africa, such as Chad, remain genetically poorly investigated. Here, we use genotype data from 480 samples from Chad, the Near East, and southern Europe, as well as whole-genome sequencing from 19 of them, to show that many populations today derive their genomes from ancient African-Eurasian admixtures. We found evidence of early Eurasian backflow to Africa in people speaking the unclassified isolate Laal language in southern Chad and estimate from linkage-disequilibrium decay that this occurred 4,750-7,200 years ago. It brought to Africa a Y chromosome lineage (R1b-V88) whose closest relatives are widespread in present-day Eurasia; we estimate from sequence data that the Chad R1b-V88 Y chromosomes coalesced 5,700-7,300 years ago. This migration could thus have originated among Near Eastern farmers during the African Humid Period. We also found that the previously documented Eurasian backflow into Africa, which occurred ∼3,000 years ago and was thought to be mostly limited to East Africa, had a more westward impact affecting populations in northern Chad, such as the Toubou, who have 20%-30% Eurasian ancestry today. We observed a decline in heterozygosity in admixed Africans and found that the Eurasian admixture can bias inferences on their coalescent history and confound genetic signals from adaptation and archaic introgression. Copyright © 2016 The Author(s). Published by Elsevier Inc. All rights reserved.

  16. Comparison of the Microbial Diversity and Abundance Between the Freshwater Land-Locked Lakes of Schirmacher Oasis and the Perennially Ice-Covered Lake Untersee in East Antarctica

    NASA Technical Reports Server (NTRS)

    Huang, Jonathan; Hoover, Richard B.; Swain, Ashit; Murdock, Chris; Bej, Asim K.

    2010-01-01

    Extreme conditions such as low temperature, dryness, and constant UV-radiation in terrestrial Antarctica are limiting factors of the survival of microbial populations. The objective of this study was to investigate the microbial diversity and enumeration between the open water lakes of Schirmacher Oasis and the permanently ice-covered Lake Untersee. The lakes in Schirmacher Oasis possessed abundant and diverse group of microorganisms compared to the Lake Untersee. Furthermore, the microbial diversity between two lakes in Schirmacher Oasis (Lake L27C and L47) was compared by culture-based molecular approach. It was determined that L27Chad a richer microbial diversity representing 5 different phyla and 7 different genera. In contrast L47 consisted of 4 different phyla and 6 different genera. The difference in microbial community could be due to the wide range of pH between L27C (pH 9.1) and L47 (pH 5.7). Most of the microbes isolated from these lakes consisted of adaptive biological pigmentation. Characterization of the microbial community found in the freshwater lakes of East Antarctica is important because it gives a further glimpse into the adaptation and survival strategies found in extreme conditions.

  17. Use of ChAd3-EBO-Z Ebola virus vaccine in Malian and US adults, and boosting of Malian adults with MVA-BN-Filo: a phase 1, single-blind, randomised trial, a phase 1b, open-label and double-blind, dose-escalation trial, and a nested, randomised, double-blind, placebo-controlled trial.

    PubMed

    Tapia, Milagritos D; Sow, Samba O; Lyke, Kirsten E; Haidara, Fadima Cheick; Diallo, Fatoumata; Doumbia, Moussa; Traore, Awa; Coulibaly, Flanon; Kodio, Mamoudou; Onwuchekwa, Uma; Sztein, Marcelo B; Wahid, Rezwanul; Campbell, James D; Kieny, Marie-Paule; Moorthy, Vasee; Imoukhuede, Egeruan B; Rampling, Tommy; Roman, Francois; De Ryck, Iris; Bellamy, Abbie R; Dally, Len; Mbaya, Olivier Tshiani; Ploquin, Aurélie; Zhou, Yan; Stanley, Daphne A; Bailer, Robert; Koup, Richard A; Roederer, Mario; Ledgerwood, Julie; Hill, Adrian V S; Ballou, W Ripley; Sullivan, Nancy; Graham, Barney; Levine, Myron M

    2016-01-01

    The 2014 west African Zaire Ebola virus epidemic prompted worldwide partners to accelerate clinical development of replication-defective chimpanzee adenovirus 3 vector vaccine expressing Zaire Ebola virus glycoprotein (ChAd3-EBO-Z). We aimed to investigate the safety, tolerability, and immunogenicity of ChAd3-EBO-Z in Malian and US adults, and assess the effect of boosting of Malians with modified vaccinia Ankara expressing Zaire Ebola virus glycoprotein and other filovirus antigens (MVA-BN-Filo). In the phase 1, single-blind, randomised trial of ChAd3-EBO-Z in the USA, we recruited adults aged 18-65 years from the University of Maryland medical community and the Baltimore community. In the phase 1b, open-label and double-blind, dose-escalation trial of ChAd3-EBO-Z in Mali, we recruited adults 18-50 years of age from six hospitals and health centres in Bamako (Mali), some of whom were also eligible for a nested, randomised, double-blind, placebo-controlled trial of MVA-BN-Filo. For randomised segments of the Malian trial and for the US trial, we randomly allocated participants (1:1; block size of six [Malian] or four [US]; ARB produced computer-generated randomisation lists; clinical staff did randomisation) to different single doses of intramuscular immunisation with ChAd3-EBO-Z: Malians received 1 × 10(10) viral particle units (pu), 2·5 × 10(10) pu, 5 × 10(10) pu, or 1 × 10(11) pu; US participants received 1 × 10(10) pu or 1 × 10(11) pu. We randomly allocated Malians in the nested trial (1:1) to receive a single dose of 2 × 10(8) plaque-forming units of MVA-BN-Filo or saline placebo. In the double-blind segments of the Malian trial, investigators, clinical staff, participants, and immunology laboratory staff were masked, but the study pharmacist (MK), vaccine administrator, and study statistician (ARB) were unmasked. In the US trial, investigators were not masked, but participants were. Analyses were per protocol. The primary outcome was safety, measured

  18. Improved harmonisation from policy dialogue? Realist perspectives from Guinea and Chad.

    PubMed

    Kwamie, Aku; Nabyonga-Orem, Juliet

    2016-07-18

    Harmonisation is a key principle of the Paris Declaration. The Universal Health Coverage (UHC) Partnership, an initiative of the European Union, the Government of Luxembourg and the World Health Organization, supported health policy dialogues between 2012 and 2015 in identified countries in the WHO African Region. The UHC Partnership has amongst its key objectives to strengthen national health policy development. In Guinea and Chad, policy dialogue focused on elaborating the national health plan and other key documents. This study is an analytical reflection inspired by realist evaluative approaches to understand whether policy dialogue led to improved harmonisation amongst health actors in Guinea and Chad, and if so, how and why. Interviews were conducted in Guinea and Chad with key informants at the national and sub-national government levels, civil society, and development partners. A review of relevant policy documents and reports was added to data collection to construct a full picture of the policy dialogue process. Context-mechanism-outcome configurations were used as the realist framework to guide the analysis on how participants' understanding of what policy dialogue was and the way the policy dialogue process unfolded led to improved harmonisation. Improved harmonisation as a result of policy dialogue was perceived to be stronger in Guinea than in Chad. While in both countries the participants held a shared view of what policy dialogue was and what it could achieve, and both policy dialogue processes were considered to be well implemented (i.e., well-facilitated, evidence-based, participatory, and consisted of recurring meetings and activities), certain contextual factors in Chad tempered the view of harmonisation as having improved. These were the pre-existence of dialogic policy processes that had exposed the actors to the potential that policy dialogue could have; a focus on elaborating provincial level strategies, which gave the sense that the process

  19. Dr. Chad E. Finn, 2013 Wilder Medal Recipient

    USDA-ARS?s Scientific Manuscript database

    Dr. Chad Finn took over the leadership of the USDA-ARS small fruit breeding program in Corvallis, Oregon in 1993 after three years working as an extension horticulturist in the Department of Horticulture at the University of Missouri, Columbia. Since taking over this program he has developed what is...

  20. [Recent Advances in Vaccines and Drugs Against the Ebola Virus].

    PubMed

    Zhu, Xiang; Yao, Chenguang; Wei, Yanhong; Kou, Zheng; Hu, Kanghong

    2015-05-01

    The Ebola virus belongs to the Filovirus family, which causes Ebola hemorrhagic fever (mortality, 25%-90%). An outbreak of infection by the Ebola virus is sweeping across West Africa, leading to high mortality and worldwide panic. The Ebola virus has caused a serious threat to public health, so intensive scientific studies have been carried out. Several vaccines (e.g., rVSV-ZEBOV, ChAd3-ZEBOV) have been put into clinical trials and antiviral drugs (e.g., TKM-Ebola, ZMAPP) have been administered in the emergency setting to patients infected by the Ebola virus. Here, recent advances in vaccines and drugs against the Ebola virus are reviewed.

  1. Riparian ecosystem resilience and livelihood strategies under test: lessons from Lake Chilwa in Malawi and other lakes in Africa.

    PubMed

    Kafumbata, Dalitso; Jamu, Daniel; Chiotha, Sosten

    2014-04-05

    This paper reviews the importance of African lakes and their management challenges. African inland lakes contribute significantly to food security, livelihoods and national economies through direct exploitation of fisheries, water resources for irrigation and hydropower generation. Because of these key contributions, the ecosystem services provided are under significant stress mainly owing to high demand by increasing populations, negative anthropogenic impacts on lake catchments and high levels of poverty which result in unsustainable use. Climate variability exacerbates the stress on these ecosystems. Current research findings show that the lakes cannot sustain further development activities on the scale seen over the past few decades. Millions of people are at risk of losing livelihoods through impacts on livestock and wildlife. The review further shows that the problems facing these lakes are beyond the purview of current management practices. A much better understanding of the interactions and feedbacks between different components of the lake socio-ecological systems is needed to address the complex challenges of managing these ecosystem services. This review suggests that the three small wetlands of Chad, Chilwa and Naivasha provide an opportunity for testing novel ideas that integrate sustainability of natural resource management with livelihoods in order to inform policy on how future land use and climatic variability will affect both food security and the ecosystem services associated with it.

  2. Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus (VHSV) isolate from USA

    PubMed Central

    Ammayappan, Arun; Vakharia, Vikram N

    2009-01-01

    Background Viral hemorrhagic septicemia virus (VHSV) is a highly contagious viral disease of fresh and saltwater fish worldwide. VHSV caused several large scale fish kills in the Great Lakes area and has been found in 28 different host species. The emergence of VHS in the Great Lakes began with the isolation of VHSV from a diseased muskellunge (Esox masquinongy) caught from Lake St. Clair in 2003. VHSV is a member of the genus Novirhabdovirus, within the family Rhabdoviridae. It has a linear single-stranded, negative-sense RNA genome of approximately 11 kbp, with six genes. VHSV replicates in the cytoplasm and produces six monocistronic mRNAs. The gene order of VHSV is 3'-N-P-M-G-NV-L-5'. This study describes molecular characterization of the Great Lakes VHSV strain (MI03GL), and its phylogenetic relationships with selected European and North American isolates. Results The complete genomic sequences of VHSV-MI03GL strain was determined from cloned cDNA of six overlapping fragments, obtained by RT-PCR amplification of genomic RNA. The complete genome sequence of MI03GL comprises 11,184 nucleotides (GenBank GQ385941) with the gene order of 3'-N-P-M-G-NV-L-5'. These genes are separated by conserved gene junctions, with di-nucleotide gene spacers. The first 4 nucleotides at the termini of the VHSV genome are complementary and identical to other novirhadoviruses genomic termini. Sequence homology and phylogenetic analysis show that the Great Lakes virus is closely related to the Japanese strains JF00Ehi1 (96%) and KRRV9822 (95%). Among other novirhabdoviruses, VHSV shares highest sequence homology (62%) with snakehead rhabdovirus. Conclusion Phylogenetic tree obtained by comparing 48 glycoprotein gene sequences of different VHSV strains demonstrate that the Great Lakes VHSV is closely related to the North American and Japanese genotype IVa, but forms a distinct genotype IVb, which is clearly different from the three European genotypes. Molecular characterization of the

  3. Detection of tilapia lake virus (TiLV) infection by PCR in farmed and wild Nile tilapia (Oreochromis niloticus) from Lake Victoria.

    PubMed

    Mugimba, K K; Chengula, A A; Wamala, S; Mwega, E D; Kasanga, C J; Byarugaba, D K; Mdegela, R H; Tal, S; Bornstein, B; Dishon, A; Mutoloki, S; David, L; Evensen, Ø; Munang'andu, H M

    2018-02-23

    Tilapia lake virus disease (TiLVD) has emerged to be an important viral disease of farmed Nile tilapia (Oreochromis niloticus) having the potential to impede expansion of aquaculture production. There is a need for rapid diagnostic tools to identify infected fish to limit the spread in individual farms. We report the first detection of TiLV infection by PCR in farmed and wild Nile tilapia from Lake Victoria. There was no difference in prevalence between farmed and wild fish samples (p = .65), and of the 442 samples examined from 191 fish, 28 were positive for TiLV by PCR. In terms of tissue distribution, the head kidney (7.69%, N = 65) and spleen (10.99%, N = 191), samples had the highest prevalence (p < .0028) followed by heart samples (3.45%, N = 29). Conversely, the prevalence was low in the liver (0.71%, N = 140) and absent in brain samples (0.0%, N = 17), which have previously been shown to be target organs during acute infections. Phylogenetic analysis showed homology between our sequences and those from recent outbreaks in Israel and Thailand. Given that these findings were based on nucleic acid detection by PCR, future studies should seek to isolate the virus from fish in Lake Victoria and show its ability to cause disease and virulence in susceptible fish. © 2018 John Wiley & Sons Ltd.

  4. Crater palaeolakes in the Tibesti mountains (Central Sahara, North Chad) - New insights into past Saharan climates

    NASA Astrophysics Data System (ADS)

    Kröpelin, Stefan; Dinies, Michèle; Sylvestre, Florence; Hoelzmann, Philipp

    2016-04-01

    For the first time continuous lacustrine sections were sampled from the volcanic Tibesti Mountains (Chad): In the 900 m deep crater of Trou au Natron at Pic Toussidé (3,315 m a.s.l.) and from the 800 m deep Era Kohor, the major sub-caldera of Emi Koussi (3,445 m a.s.l.). The remnant diatomites on their slopes are located 360 m (Trou au Natron) and 125 m (Era Kohor) above the present day bottom of the calderas. These sediments from highly continental positions in the central Sahara are keys for the reconstruction of the last climatic cycles (Kröpelin et al. 2015). We report first results from sedimentary-geochemical (total organic and total inorganic carbon contents; total nitrogen; major elements; mineralogy) and palynological analyses for palaeo-environmental interpretations. The diatomites from the Trou au Natron comprise 330 cm of mostly calcitic sediments with relatively low organic carbon (<2.5 %) and strongly varying aragonite and gypsum contents. Major elements (Ca, Fe, K, Mg, Mn, Na, P, S, Sr), elemental ratios (Sr/Ca, Mg/Ca, Fe/Mn) and the mineralogy are used to interpret the lake's salinity, productivity and ecological conditions. Trilete spores are preserved throughout the sequence, probably reflecting local moss/fern stands. Regional pollen rain-e.g. grasses and wormwood-is scarcely represented. Golden algae dominate in the lower section. The results of the first palynological samples suggest a small sedimentation basin. Two 14C-dated charcoals out of the upper part of the section indicate mid-Holocene ages and a linear extrapolation based on a sediment accumulation rate of 1.4mma-1 would lead to tentative dates of ~8650 cal a BP for basal lacustrine sediments and ~4450 cal a BP for the cessation of this lacustrine sequence. The diatomites from the Era Kohor reflect a suite of sections that in total sum up to 145 cm of mostly silica-based sediments with very low carbon contents (< 2% TC). Calcite dominated sediments are only present in the topmost 15

  5. Area Handbook Series: Chad, A Country Study

    DTIC Science & Technology

    1988-12-01

    northern and central interests. As dis- affection in these regions increased, in the late 1960s dissident groups formed an antigovernment coalition, the...decades-might finally ensue. December 13, 1988 * * seea eet After the research for this book was completed, several events occurred that greatly affected ...Chad’s geographic position along major trans-Saharan trade routes has also affected its historical development. In early times, trade consisted of

  6. Human and bovine viruses and bacteria at three Great Lakes beaches: Environmental variable associations and health risk

    USGS Publications Warehouse

    Corsi, Steven R.; Borchardt, Mark A.; Carvin, Rebecca B.; Burch, Tucker R; Spencer, Susan K.; Lutz, Michelle A.; McDermott, Colleen M.; Busse, Kimberly M.; Kleinheinz, Gregory; Feng, Xiaoping; Zhu, Jun

    2016-01-01

    Waterborne pathogens were measured at three beaches in Lake Michigan, environmental factors for predicting pathogen concentrations were identified, and the risk of swimmer infection and illness was estimated. Waterborne pathogens were detected in 96% of samples collected at three Lake Michigan beaches in summer, 2010. Samples were quantified for 22 pathogens in four microbial categories (human viruses, bovine viruses, protozoa, and pathogenic bacteria). All beaches had detections of human and bovine viruses and pathogenic bacteria indicating influence of multiple contamination sources at these beaches. Occurrence ranged from 40 to 87% for human viruses, 65–87% for pathogenic bacteria, and 13–35% for bovine viruses. Enterovirus, adenovirus A, Salmonella spp., Campylobacter jejuni, bovine polyomavirus, and bovine rotavirus A were present most frequently. Variables selected in multiple regression models used to explore environmental factors that influence pathogens included wave direction, cloud cover, currents, and water temperature. Quantitative Microbial Risk Assessment was done for C. jejuni, Salmonella spp., and enteroviruses to estimate risk of infection and illness. Median infection risks for one-time swimming events were approximately 3 × 10–5, 7 × 10–9, and 3 × 10–7 for C. jejuni, Salmonella spp., and enteroviruses, respectively. Results highlight the importance of investigating multiple pathogens within multiple categories to avoid underestimating the prevalence and risk of waterborne pathogens.

  7. Temporal variation in viral hemorrhagic septicemia virus antibodies in freshwater drum (Aplodinotus grunniens) indicates cyclic transmission in Lake Winnebago, Wisconsin

    USGS Publications Warehouse

    Wilson-Rothering, Anna; Marcquenski, Susan; Koenigs, Ryan P.; Bruch, Ronald; Kamke, Kendall; Isermann, Daniel A.; Thurman, Andrew; Toohey-Kurth, Kathy; Goldberg, Tony

    2015-01-01

    Viral hemorrhagic septicemia virus (VHSV) is an emerging pathogen that causes mass mortality in multiple fish species. In 2007, the Great Lakes freshwater strain, type IVb, caused a large die-off of freshwater drum (Aplodinotus grunniens) in Lake Winnebago, Wisconsin, USA. To evaluate the persistence and transmission of VHSV, freshwater drum from Lake Winnebago were tested for antibodies to the virus using recently developed virus neutralization (VN) and enzyme-linked immunosorbent (ELISA) assays. Samples were also tested by real-time reverse transcription-PCR (rRT-PCR) to detect viral RNA. Of 548 serum samples tested, 44 (8.03%) were positive by VN (titers ranging from 1:16 to 1:1,024) and 45 (8.21%) were positive by ELISA, including 7 fish positive by both assays. Antibody prevalence increased with age and was higher in one northwestern area of Lake Winnebago than in other areas. Of 3,864 tissues sampled from 551 fish, 1 spleen and 1 kidney sample from a single adult female fish collected in the spring of 2012 tested positive for VHSV by rRT-PCR, and serum from the same fish tested positive by VN and ELISA. These results suggest that VHSV persists and viral transmission may be active in Lake Winnebago even in years following outbreaks and that wild fish may survive VHSV infection and maintain detectable antibody titers while harboring viral RNA. Influxes of immunologically naive juvenile fish through recruitment may reduce herd immunity, allow VHSV to persist, and drive superannual cycles of transmission that may sporadically manifest as fish kills.

  8. Temporal Variation in Viral Hemorrhagic Septicemia Virus Antibodies in Freshwater Drum (Aplodinotus grunniens) Indicates Cyclic Transmission in Lake Winnebago, Wisconsin

    PubMed Central

    Wilson-Rothering, Anna; Marcquenski, Susan; Koenigs, Ryan; Bruch, Ronald; Kamke, Kendall; Isermann, Daniel; Thurman, Andrew; Toohey-Kurth, Kathy

    2015-01-01

    Viral hemorrhagic septicemia virus (VHSV) is an emerging pathogen that causes mass mortality in multiple fish species. In 2007, the Great Lakes freshwater strain, type IVb, caused a large die-off of freshwater drum (Aplodinotus grunniens) in Lake Winnebago, Wisconsin, USA. To evaluate the persistence and transmission of VHSV, freshwater drum from Lake Winnebago were tested for antibodies to the virus using recently developed virus neutralization (VN) and enzyme-linked immunosorbent (ELISA) assays. Samples were also tested by real-time reverse transcription-PCR (rRT-PCR) to detect viral RNA. Of 548 serum samples tested, 44 (8.03%) were positive by VN (titers ranging from 1:16 to 1:1,024) and 45 (8.21%) were positive by ELISA, including 7 fish positive by both assays. Antibody prevalence increased with age and was higher in one northwestern area of Lake Winnebago than in other areas. Of 3,864 tissues sampled from 551 fish, 1 spleen and 1 kidney sample from a single adult female fish collected in the spring of 2012 tested positive for VHSV by rRT-PCR, and serum from the same fish tested positive by VN and ELISA. These results suggest that VHSV persists and viral transmission may be active in Lake Winnebago even in years following outbreaks and that wild fish may survive VHSV infection and maintain detectable antibody titers while harboring viral RNA. Influxes of immunologically naive juvenile fish through recruitment may reduce herd immunity, allow VHSV to persist, and drive superannual cycles of transmission that may sporadically manifest as fish kills. PMID:26135873

  9. Genetic characterization of H9N2 avian influenza virus in plateau pikas in the Qinghai Lake region of China.

    PubMed

    Yan, Yan; Gu, Jin-Yan; Yuan, Zhuang-Chuan; Chen, Xin-Yu; Li, Zeng-Kui; Lei, Jing; Hu, Bo-Li; Yan, Li-Ping; Xing, Gang; Liao, Min; Zhou, Ji-Yong

    2017-04-01

    Qinghai Lake is a major migratory-bird breeding site that has experienced several highly pathogenic avian influenza virus (AIV) epizootics. Plateau pikas (Ochotona curzoniae) have previously been implicated in the ecology of avian influenza virus in this region. We first isolated an H9N2 AIV (A/Pika/Menyuan/01/2008) from plateau pikas between November 2008 and October 2009. Sequence analysis showed that the A/Pika/Menyuan/01/2008 AIV was closely related to the H9N2 AIV strain (A/Turkey/Wisconsin/ 1/1966). Our findings suggested that plateau pikas may contribute to AIV epidemiology in the Qinghai Lake region.

  10. Advanced chronic kidney disease in non-valvular atrial fibrillation: extending the utility of R2CHADS2 to patients with advanced renal failure.

    PubMed

    Bautista, Josef; Bella, Archie; Chaudhari, Ashok; Pekler, Gerald; Sapra, Katherine J; Carbajal, Roger; Baumstein, Donald

    2015-04-01

    The R2CHADS2 is a new prediction rule for stroke risk in atrial fibrillation (AF) patients wherein R stands for renal risk. However, it was created from a cohort that excluded patients with advanced renal failure (defined as glomerular filtration rate of <30 mL/min). Our study extends the use of R2CHADS2 to patients with advanced renal failure and aims to compare its predictive power against the currently used CHADS and CHA2DS2VaSc. This retrospective cohort study analyzed the 1-year risk for stroke of the 524 patients with AF at Metropolitan Hospital Center. AUC and C statistics were calculated using three groups: (i) the entire cohort including patients with advanced renal failure, (ii) a cohort excluding patients with advanced renal failure and (iii) all patients with GFR < 30 mL/min only. R2CHADS2, as a predictor for stroke risk, consistently performs better than CHADS2 and CHA2DS2VsC in groups 1 and 2. The C-statistic was highest in R2CHADS compared with CHADS or CHADSVASC in group 1 (0.718 versus 0.605 versus 0.602) and in group 2 (0.724 versus 0.584 versus 0.579). However, there was no statistically significant difference in group 3 (0.631 versus 0.629 versus 0.623). Our study supports the utility of R2CHADS2 as a clinical prediction rule for stroke risk in patients with advanced renal failure.

  11. Varroa destructor Macula-like virus, Lake Sinai virus and other new RNA viruses in wild bumblebee hosts (Bombus pascuorum, Bombus lapidarius and Bombus pratorum).

    PubMed

    Parmentier, Laurian; Smagghe, Guy; de Graaf, Dirk C; Meeus, Ivan

    2016-02-01

    Pollinators such as bumblebees (Bombus spp.) are in decline worldwide which poses a threat not only for ecosystem biodiversity but also to human crop production services. One main cause of pollinator decline may be the infection and transmission of diseases including RNA viruses. Recently, new viruses have been discovered in honeybees, but information on the presence of these in wild bumblebees is largely not available. In this study, we investigated the prevalence of new RNA viruses in Bombus species, and can report for the first time Varroa destructor Macula-like virus (VdMLV) and Lake Sinai virus (LSV) infection in multiple wild bumblebee hosts of Bombus pascuorum, Bombus lapidarius and Bombus pratorum. We sampled in 4 locations in Flanders, Belgium. Besides, we confirmed Slow bee paralysis virus (SBPV) in wild bumblebees, but no positive samples were obtained for Big Sioux river virus (BSRV). Secondly, we screened for the influence of apiaries on the prevalence of these viruses. Our results indicated a location effect for the prevalence of VdMLV in Bombus species, with a higher prevalence in the proximity of honeybee apiaries mainly observed in one location. For LSV, the prevalence was not different in the proximity or at a 1.5 km-distance of apiaries, but we reported a different isolate with similarities to LSV-2 and "LSV-clade A" as described by Ravoet et al. (2015), which was detected both in Apis mellifera and Bombus species. In general, our results indicate the existence of a disease pool of new viruses that seems to be associated to a broad range of Apoidae hosts, including multiple Bombus species. Copyright © 2015 Elsevier Inc. All rights reserved.

  12. Potential distribution of the viral haemorrhagic septicaemia virus in the Great Lakes region

    USGS Publications Warehouse

    Escobar, Luis E.; Kurath, Gael; Escobar-Dodero, Joaquim; Craft, Meggan E.; Phelps, Nicholas B.D.

    2017-01-01

    Viral haemorrhagic septicaemia virus (VHSV) genotype IVb has been responsible for large-scale fish mortality events in the Great Lakes of North America. Anticipating the areas of potential VHSV occurrence is key to designing epidemiological surveillance and disease prevention strategies in the Great Lakes basin. We explored the environmental features that could shape the distribution of VHSV, based on remote sensing and climate data via ecological niche modelling. Variables included temperature measured during the day and night, precipitation, vegetation, bathymetry, solar radiation and topographic wetness. VHSV occurrences were obtained from available reports of virus confirmation in laboratory facilities. We fit a Maxent model using VHSV-IVb reports and environmental variables under different parameterizations to identify the best model to determine potential VHSV occurrence based on environmental suitability. VHSV reports were generated from both passive and active surveillance. VHSV occurrences were most abundant near shore sites. We were, however, able to capture the environmental signature of VHSV based on the environmental variables employed in our model, allowing us to identify patterns of VHSV potential occurrence. Our findings suggest that VHSV is not at an ecological equilibrium and more areas could be affected, including areas not in close geographic proximity to past VHSV reports.

  13. Detection of Viral Hemorrhagic Septicemia Virus (VHSV) from Diporeia spp. (Pontoporeiidae, Amphipoda) in the Laurentian Great Lakes, USA

    PubMed Central

    2011-01-01

    The mode of viral hemorrhagic septicemia virus (VHSV) transmission in the Great Lakes basin is largely unknown. In order to assess the potential role of macroinvertebrates in VHSV transmission, Diporeia spp., a group of amphipods that are preyed upon by a number of susceptible Great Lakes fishes, were collected from seven locations in four of the Great Lakes and analyzed for the presence of VHSV. It was demonstrated that VHSV is present in some Diporeia spp. samples collected from lakes Ontario, Huron, and Michigan, but not from Lake Superior. Phylogenetic comparison of partial nucleoprotein (N) gene sequences (737 base pairs) of the five isolates to sequences of 13 other VHSV strains showed the clustering of Diporeia spp. isolates with the VHSV genotype IVb. This study reports the first incidence of a fish-pathogenic rhabdovirus being isolated from Diporeia, or any other crustacean and underscores the role macroinvertebrates may play in VHSV ecology. PMID:21210995

  14. A cost-utility analysis for catheter ablation of atrial fibrillation in combination with warfarin and dabigatran based on the CHADS2 score in Japan.

    PubMed

    Kimura, Takehiro; Igarashi, Ataru; Ikeda, Shunya; Nakajima, Kazuaki; Kashimura, Shin; Kunitomi, Akira; Katsumata, Yoshinori; Nishiyama, Takahiko; Nishiyama, Nobuhiro; Fukumoto, Kotaro; Tanimoto, Yoko; Aizawa, Yoshiyasu; Fukuda, Keiichi; Takatsuki, Seiji

    2017-01-01

    We aimed to clarify the cost-effectiveness of an expensive combination therapy for atrial fibrillation (AF) using both catheter ablation and dabigatran compared with warfarin at each CHADS 2 score for patients in Japan. A Markov model was constructed to analyze costs and quality-adjusted life years associated with AF therapeutic options with a time horizon of 10 years. The target population was 60-year-old patients with paroxysmal AF. The indication for anticoagulation was determined according to the Japanese guideline. Anticoagulation-related data were derived from the RE-LY study and the AF recurrence rate was set at 2.7% per month during the first 12 months and at 0.40% per month afterwards. Stroke risk was determined according to AF recurrence, anticoagulation, and CHADS 2 score. The risks for stroke recurrence and stroke death were also considered. Costs were calculated from the healthcare payer's perspective, and only direct medical costs were included. Warfarin was the most preferred option for patients with a CHADS 2 score of 0 from a health economics aspect. Ablation under warfarin was preferred for a CHADS 2 score of 1-3, while ablation under dabigatran was preferred for a CHADS 2 score ≥4. The quality of life score for AF had the largest impact on the incremental cost-effectiveness ratios in the analysis between the anticoagulation arm and the anticoagulation+ablation arm for a CHADS 2 score of 2. Within the range of the Japanese willingness-to-pay threshold (¥5,000,000), the ablation+warfarin arm became the best option with its probability of 81.7% for a CHADS 2 score of 2; the dabigatran+ablation arm was the most preferred option with its probability of 56.1% for a CHADS 2 score of 4. Ablation under dabigatran therapy is an expensive therapeutic option, but it might benefit patients with a low quality of life and a high CHADS 2 score. Copyright © 2016 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.

  15. Detection of Viral Hemorrhagic Septicemia Virus by Quantitative Reverse Transcription Polymerase Chain Reaction from Two Fish Species at Two Sites in Lake Superior

    USGS Publications Warehouse

    Cornwell, Emily R.; Eckerlin, Geofrey E.; Getchell, Rodman G.; Groocock, Geoffrey H.; Thompson, Tarin M.; Batts, William N.; Casey, Rufina N.; Kurath, Gael; Winton, James R.; Bowser, Paul R.; Bain, Mark B.; Casey, James W.

    2011-01-01

    Viral hemorrhagic septicemia virus (VHSV) was first detected in the Laurentian Great Lakes in 2005 during a mortality event in the Bay of Quinte, Lake Ontario. Subsequent analysis of archived samples determined that the first known isolation of VHSV in the Laurentian Great Lakes was from a muskellunge Esox masquinongy collected in Lake St. Clair in 2003. By the end of 2008, mortality events and viral isolations had occurred in all of the Laurentian Great Lakes except Lake Superior. In 2009, a focused disease surveillance program was designed to determine whether VHSV was also present in Lake Superior. In this survey, 874 fish from 7 sites along the U.S. shoreline of Lake Superior were collected during June 2009. Collections were focused on nearshore species known to be susceptible to VHSV. All fish were dissected individually by using aseptic techniques and were tested for the presence of VHSV genetic material by use of a quantitative reverse transcription (qRT) polymerase chain reaction (PCR) targeting the viral nucleoprotein gene. Seventeen fish from two host species at two different sites tested positive at low levels for VHSV. All attempts to isolate virus in cell culture were unsuccessful. However, the presence of viral RNA was confirmed independently in five fish by using a nested PCR that targeted the glycoprotein (G) gene. Partial G gene sequences obtained from three fish were identical to the corresponding sequence from the original 2003 VHSV isolate (MI03) from muskellunge. These detections represent the earliest evidence for the presence of VHSV in Lake Superior and illustrate the utility of the highly sensitive qRT-PCR assay for disease surveillance in aquatic animals.

  16. Petroleum Systems of the Nigerian Sector of Chad Basin: Insights from Field and Subsurface Data

    NASA Astrophysics Data System (ADS)

    Suleiman, A. A.; Nwaobi, G. O.; Bomai, A.; Dauda, R.; Bako, M. D.; Ali, M. S.; Moses, S. D.

    2017-12-01

    A.A. Suleiman, A. Bomai, R. Dauda, O.G. NwaobiNigerian National Petroleum CorporationAbstract:Formation of the West and Central African Rift systems (WCARS) reflects intra-plate deformation linked to the Early to Late Cretaceous opening of South Atlantic Ocean. From an economic point of view, the USGS (2010) estimated Chad Basin, which is part of WCARS rift system to contain, up to 2.32 BBO and 14.62 TCF. However, there has been no exploration success in the Nigerian sector of the Chad Basin principally because of a poor understanding of the basin tectono-stratigraphic evolution and petroleum system development. In this study, we use 3D seismic, geochemical and field data to construct a tectono-stratigraphic framework of the Nigerian sector of Chad Basin; within this framework we then investigate the basins petroleum system development. Our analysis suggests two key plays exist in the basin, Lower and Upper Cretaceous plays. Pre-Bima lacustrine shale and the Gongila Formation constitute the prospective source rocks for the Lower Cretaceous play, whereas the Fika Shale may provide the source, for the Upper Cretaceous play. Source rock hydrocarbon modeling indicates possible oil and gas generation and expulsion from the lacustrine shales and Fika Shale in Cretaceous and Tertiary times respectively. Bima Sandstone and weathered basement represent prospective reservoirs for the Lower Cretaceous play and intra-Fika sandstone beds for the Upper Cretaceous play. We identify a range of trapping mechanisms such as inversion-related anticlines. Shales of the Gongila Formation provide the top sealing for the Lower Cretaceous play. Our field observations have proved presence of the key elements of the petroleum system in the Nigerian Sector of the Chad Basin. It has also demonstrated presence of igneous intrusions in the stratigraphy of the basin that we found to influence the hydrocarbon potential of the basin through source rock thermal maturity and degradation. Our study

  17. Predictive value of CHADS2 and CHA2DS2-VASc scores for acute myocardial infarction in patients with atrial fibrillation.

    PubMed

    Pang, Hui; Han, Bing; Fu, Qiang; Zong, Zhenkun

    2017-07-05

    The presence of acute myocardial infarction (AMI) confers a poor prognosis in atrial fibrillation (AF), associated with increased mortality dramatically. This study aimed to evaluate the predictive value of CHADS 2 and CHA 2 DS 2 -VASc scores for AMI in patients with AF. This retrospective study enrolled 5140 consecutive nonvalvular AF patients, 300 patients with AMI and 4840 patients without AMI. We identified the optimal cut-off values of the CHADS 2 and CHA 2 DS 2 -VASc scores each based on receiver operating characteristic curves to predict the risk of AMI. Both CHADS 2 score and CHA 2 DS 2 -VASc score were associated with an increased odds ratio of the prevalence of AMI in patients with AF, after adjustment for hyperlipidaemia, hyperuricemia, hyperthyroidism, hypothyroidism and obstructive sleep apnea. The present results showed that the area under the curve (AUC) for CHADS 2 score was 0.787 with a similar accuracy of the CHA 2 DS 2 -VASc score (AUC 0.750) in predicting "high-risk" AF patients who developed AMI. However, the predictive accuracy of the two clinical-based risk scores was fair. The CHA 2 DS 2 -VASc score has fair predictive value for identifying high-risk patients with AF and is not significantly superior to CHADS 2 in predicting patients who develop AMI.

  18. Distribution of an invasive aquatic pathogen (viral hemorrhagic septicemia virus) in the Great Lakes and its relationship to shipping

    USGS Publications Warehouse

    Bain, Mark B.; Cornwell, Emily R.; Hope, Kristine M.; Eckerlin, Geofrey E.; Casey, Rufina N.; Groocock, Geoffrey H.; Getchell, Rodman G.; Bowser, Paul R.; Winton, James R.; Batts, William N.; Cangelosi, Allegra; Casey, James W.

    2010-01-01

    Viral hemorrhagic septicemia virus (VHSV) is a rhabdovirus found in fish from oceans of the northern hemisphere and freshwaters of Europe. It has caused extensive losses of cultured and wild fish and has become established in the North American Great Lakes. Large die-offs of wild fish in the Great Lakes due to VHSV have alarmed the public and provoked government attention on the introduction and spread of aquatic animal pathogens in freshwaters. We investigated the relations between VHSV dispersion and shipping and boating activity in the Great Lakes by sampling fish and water at sites that were commercial shipping harbors, recreational boating centers, and open shorelines. Fish and water samples were individually analyzed for VHSV using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and cell culture assays. Of 1,221 fish of 17 species, 55 were VHSV positive with highly varied qRT-PCR titers (1 to 5,950,000 N gene copies). The detections of VHSV in fish and water samples were closely associated and the virus was detected in 21 of 30 sites sampled. The occurrence of VHSV was not related to type of site or shipping related invasion hotspots. Our results indicate that VHSV is widely dispersed in the Great Lakes and is both an enzootic and epizootic pathogen. We demonstrate that pathogen distribution information could be developed quickly and is clearly needed for aquatic ecosystem conservation, management of affected populations, and informed regulation of the worldwide trade of aquatic organisms.

  19. Viral activity in two contrasting lake ecosystems.

    PubMed

    Bettarel, Yvan; Sime-Ngando, Télesphore; Amblard, Christian; Dolan, John

    2004-05-01

    For aquatic systems, especially freshwaters, there is little data on the long-term (i.e., >6-month period) and depth-related variability of viruses. In this study, we examined virus-induced mortality of heterotrophic bacteria over a 10-month period and throughout the water column in two lakes of the French Massif Central, the oligomesotrophic Lake Pavin and the eutrophic Lake Aydat. Concurrently, we estimated nonviral mortality through heterotrophic nanoflagellate and ciliate bacterivory. Overall, viral infection parameters were much less variable than bacterial production. We found that the frequency of visibly infected cells (FVIC), estimated using transmission electron microscopy, peaked in both lakes at the end of spring (May to June) and in early autumn (September to October). FVIC values were significantly higher in Lake Pavin (mean [M] = 1.6%) than in Lake Aydat (M = 1.1%), whereas the opposite trend was observed for burst sizes, which averaged 25.7 and 30.2 virus particles bacterium(-1), respectively. We detected no significant depth-related differences in FVIC or burst size. We found that in both lakes the removal of bacterial production by flagellate grazing (M(Pavin) = 37.7%, M(Aydat) = 18.5%) was nearly always more than the production removed by viral lysis (M(Pavin) = 16.2%, M(Aydat) = 19%) or ciliate grazing (M(Pavin) = 2.7%, M(Aydat) = 8.8%). However, at specific times and locations, viral lysis prevailed over protistan grazing, for example, in the anoxic hypolimnion of Lake Aydat. In addition, viral mortality represented a relatively constant mortality source in a bacterial community showing large variations in growth rate and subject to large variations in loss rates from grazers. Finally, although viruses did not represent the main agent of bacterial mortality, our data seem to show that their relative importance was higher in the less productive system.

  20. The CHADS2 Score to Predict Stroke Risk in the Absence of Atrial Fibrillation in Hypertensive Patients Aged 65 Years or Older.

    PubMed

    Morillas, Pedro; Pallarés, Vicente; Fácila, Lorenzo; Llisterri, Jose Luis; Sebastián, María Eugenia; Gómez, Manuel; Castilla, Elena; Camarasa, Raquel; Sandin, Miriam; García-Honrubia, Antonio

    2015-06-01

    The CHADS2 score is a proven, essential tool for estimating cardioembolic risk (mainly stroke) in patients with nonvalvular atrial fibrillation, with the purpose of determining the indication for anticoagulant therapy. In this study we analyzed the use of CHADS2 in hypertensive patients without known atrial fibrillation in a Mediterranean population. The study included 887 hypertensive patients aged 65 years or older without atrial fibrillation or anticoagulant therapy, who attended a medical consultation. Data on the patients' main risk factors, cardiovascular history, and medication were collected, basic laboratory analyses and electrocardiography were performed, and the CHADS2 score (heart failure, hypertension, age ≥ 75 years, diabetes mellitus, and previous stroke or transient ischemic attack) was calculated. A clinical follow-up was carried out, recording hospital admissions for a stroke or transient ischemic attack. The median duration of follow-up was 804 days. Mean age was 72.5 (SD,5.7) years, 46.6% were men, 27.8% had diabetes, and 8.6% were smokers. During follow-up, 40 patients were hospitalized for a stroke or transient ischemic attack (4.5%). The event-free survival analysis showed significant differences according to the CHADS2 score (log rank test, P < .001). On multivariate analysis, smoking and CHADS2 ≥3 were independent predictors of stroke or transient ischemic attack. The CHADS2 may be useful for estimating the risk of stroke or transient ischemic attack in hypertensive patients without known atrial fibrillation. Copyright © 2014 Sociedad Española de Cardiología. Published by Elsevier España, S.L.U. All rights reserved.

  1. Virus-bacterium coupling driven by both turbidity and hydrodynamics in an Amazonian floodplain lake.

    PubMed

    Barros, Nathan; Farjalla, Vinicius F; Soares, Maria C; Melo, Rossana C N; Roland, Fábio

    2010-11-01

    The importance of viruses in aquatic ecosystem functioning has been widely described. However, few studies have examined tropical aquatic ecosystems. Here, we evaluated for the first time viruses and their relationship with other planktonic communities in an Amazonian freshwater ecosystem. Coupling between viruses and bacteria was studied, focusing both on hydrologic dynamics and anthropogenic forced turbidity in the system (Lake Batata). Samples were taken during four hydrologic seasons at both natural and impacted sites to count virus-like particles (VLP) and bacteria. In parallel, virus-infected bacteria were identified and quantified by transmission electron microscopy (TEM). Viral abundance ranged from 0.5 × 10⁷ ± 0.2 × 10⁷ VLP ml⁻¹ (high-water season, impacted site) to 1.7 × 10⁷ ± 0.4 × 10⁷ VLP ml⁻¹ (low-water season, natural site). These data were strongly correlated with the bacterial abundance (r² = 0.84; P < 0.05), which ranged from 1.0 × 10⁶ ± 0.5 × 10⁶ cells ml⁻¹ (high water, impacted site) to 3.4 × 10⁶ ± 0.7 × 10⁶ cells ml⁻¹ (low water, natural site). Moreover, the viral abundance was weakly correlated with chlorophyll a, suggesting that most viruses were bacteriophages. TEM quantitative analyses revealed that the frequency of visibly infected cells was 20%, with 10 ± 3 phages per cell section. In general, we found a low virus-bacterium ratio (<7). Both the close coupling between the viral and bacterial abundances and the low virus-bacterium ratio suggest that viral abundance tends to be driven by the reduction of hosts for viral infection. Our results demonstrate that viruses are controlled by biological substrates, whereas in addition to grazing, bacteria are regulated by physical processes caused by turbidity, which affect underwater light distribution and dissolved organic carbon availability.

  2. Interview: An interview with Chad Mirkin: nanomedicine expert

    PubMed Central

    Mirkin, Chad

    2015-01-01

    Chad Mirkin speaks to Hannah Stanwix, Assistant Commissioning Editor Professor Chad Mirkin received his Bachelor of Science Degree in Chemistry from Dickinson College (PA, USA) in 1986. He holds a PhD in Chemistry from Pennsylvania State University (PA, USA) and was a Postdoctoral Fellow at the Massachusetts Institute of Technology (MA, USA). He subsequently moved to Northwestern University (IL, USA) as a Professor of Chemistry in 1991. In 2004, Professor Mirkin became Director of the International Institute for Nanotechnology and holds that post currently. He is also the George B Rathmann Professor of Chemistry, Professor of Chemical and Biological Engineering, Professor of Biomedical Engineering, Professor of Materials Science and Engineering and Professor of Medicine at Northwestern University. Professor Mirkin is a member of the National Academy of Engineering, the National Academy of Sciences, the Institute of Medicine, and the American Academy of Arts and Sciences. He is also currently a member of President Obama’s Council of Advisors for Science and Technology. Professor Mirkin is best known for his work on spherical nucleic acid nanoparticle conjugates and the invention of Dip-Pen Nanolithography. He has received over 70 awards and accolades for his accomplishments. Currently, based on total citations, Professor Mirkin is one of the most cited chemists and nanomedicine researchers in the world. He has authored over 500 publications, as well as over 440 patents and applications worldwide. PMID:22630148

  3. Petroleum geology of Cretaceous-Tertiary rift basins in Niger, Chad, and Central African Republic

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Genik, G.J.

    1993-08-01

    This overview of the petroleum geology of rift basins in Niger, Chad, and Central African Republic (CAR) is based on exploration work by Exxon and partners in the years 1969-1991. The work included 50,000 km of modern reflection seismic, 53 exploration wells, 1,000,000 km[sup 2] of aeromagnetic coverage, and about 10,500 km of gravity profiles. The results outline ten Cretaceous and Tertiary rift basins, which constitute a major part of the West and Central African rift system (WCARS). The rift basins derive from a multiphased geologic history dating from the Pan-African (approximately 750-550 Ma) to the Holocene. WCARS in themore » study area is divided into the West African rift subsystem (WAS) and the Central African rift subsystem (WAS) and the Central African rift subsystem (CAS). WAS basins in Niger and Chad are chiefly extensional, and are filled by up to 13,000 m of Lower Cretaceous to Holocene continental and marine clastics. The basins contain five oil (19-43[degrees]API) and two oil and gas accumulations in Upper Cretaceous and Eocene sandstone reservoirs. The hydrocarbons are sourced and sealed by Upper Cretaceous and Eocene marine and lacustrine shales. The most common structural styles and hydrocarbon traps usually are associated with normal fault blocks. CAS rift basins in Chad and CAR are extensional and transtensional, and are filled by up to 7500 m of chiefly Lower Cretaceous continental clastics. The basins contain eight oil (15-39[degrees]API) and one oil and gas discovery in Lower and Upper Cretaceous sandstone reservoirs. The hydrocarbons are sourced by Lower Cretaceous shales and sealed by interbedded lacustrine and flood-plain shales. Structural styles range from simple fault blocks through complex flower structures. The main hydrocarbon traps are in contractional anticlines. Geological conditions favor the discovery of potentially commercial volumes of oil in WCARS basins, of Niger, Chad and CAR. 108 refs., 24 figs., 4 tabs.« less

  4. Resistance and Protective Immunity in Redfish Lake Sockeye Salmon Exposed to M Type Infectious Hematopoietic Necrosis Virus (IHNV)

    USGS Publications Warehouse

    Kurath, Gael; Garver, Kyle; Purcell, Maureen K.; LaPatra, Scott E.

    2010-01-01

    Differential virulence of infectious hematopoietic necrosis virus (IHNV) isolates from the U and M phylogenetic subgroups is clearly evident in the Redfish Lake (RFL) strain of sockeye salmon Oncorhynchus nerka. In these fish, experimental immersion challenges with U isolates cause extremely high mortality and M isolates cause low or no mortality. When survivors of M virus immersion challenges were exposed to a secondary challenge with virulent U type virus they experienced high mortality, indicating that the primary M challenge did not elicit protective immunity. Delivery of a moderate dose (2 × 104 plaque-forming units [PFU]/fish) of virus by intraperitoneal injection challenge did not overcome RFL sockeye salmon resistance to M type IHNV. Injection challenge with a high dose (5 × 106 PFU/fish) of M type virus caused 10% mortality, and in this case survivors did develop protective immunity against a secondary U type virus challenge. Thus, although it is possible for M type IHNV to elicit cross-protective immunity in this disease model, it does not develop after immersion challenge despite entry, transient replication of M virus to low levels, stimulation of innate immune genes, and development of neutralizing antibodies in some fish.

  5. Quantification of virus genes provides evidence for seed-bank populations of phycodnaviruses in Lake Ontario, Canada

    PubMed Central

    Short, Cindy M; Rusanova, Oksana; Short, Steven M

    2011-01-01

    Using quantitative PCR, the abundances of six phytoplankton viruses DNA polymerase (polB) gene fragments were estimated in water samples collected from Lake Ontario, Canada over 26 months. Four of the polB fragments were most related to marine prasinoviruses, while the other two were most closely related to cultivated chloroviruses. Two Prasinovirus-related genes reached peak abundances of >1000 copies ml−1 and were considered ‘high abundance', whereas the other two Prasinovirus-related genes peaked at abundances <1000 copies ml−1 and were considered ‘low abundance'. Of the genes related to chloroviruses, one peaked at ca 1600 copies ml−1, whereas the other reached only ca 300 copies ml−1. Despite these differences in peak abundance, the abundances of all genes monitored were lowest during the late fall, winter and early spring; during these months the high abundance genes persisted at 100–1000 copies ml−1 while the low abundance Prasinovirus- and Chlorovirus-related genes persisted at fewer than ca 100 copies ml−1. Clone libraries of psbA genes from Lake Ontario revealed numerous Chlorella-like algae and two prasinophytes demonstrating the presence of candidate hosts for all types of viruses monitored. Our results corroborate recent metagenomic analyses that suggest that aquatic virus communities are composed of only a few abundant populations and many low abundance populations. Thus, we speculate that an ecologically important characteristic of phycodnavirus communities is seed-bank populations with members that can become numerically dominant when their host abundances reach appropriate levels. PMID:21124493

  6. Quantification of virus genes provides evidence for seed-bank populations of phycodnaviruses in Lake Ontario, Canada.

    PubMed

    Short, Cindy M; Rusanova, Oksana; Short, Steven M

    2011-05-01

    Using quantitative PCR, the abundances of six phytoplankton viruses DNA polymerase (polB) gene fragments were estimated in water samples collected from Lake Ontario, Canada over 26 months. Four of the polB fragments were most related to marine prasinoviruses, while the other two were most closely related to cultivated chloroviruses. Two Prasinovirus-related genes reached peak abundances of >1000 copies ml(-1) and were considered 'high abundance', whereas the other two Prasinovirus-related genes peaked at abundances <1000 copies ml(-1) and were considered 'low abundance'. Of the genes related to chloroviruses, one peaked at ca 1600 copies ml(-1), whereas the other reached only ca 300 copies ml(-1). Despite these differences in peak abundance, the abundances of all genes monitored were lowest during the late fall, winter and early spring; during these months the high abundance genes persisted at 100-1000 copies ml(-1) while the low abundance Prasinovirus- and Chlorovirus-related genes persisted at fewer than ca 100 copies ml(-1). Clone libraries of psbA genes from Lake Ontario revealed numerous Chlorella-like algae and two prasinophytes demonstrating the presence of candidate hosts for all types of viruses monitored. Our results corroborate recent metagenomic analyses that suggest that aquatic virus communities are composed of only a few abundant populations and many low abundance populations. Thus, we speculate that an ecologically important characteristic of phycodnavirus communities is seed-bank populations with members that can become numerically dominant when their host abundances reach appropriate levels.

  7. PHYSICAL ACTIVITY INDEX FOR CHILDREN: A COMPARISON OF LITERATURE VALUES AND EPA'S CHAD

    EPA Science Inventory

    The physical activity index (PAI) is a measure of an individual's energy expenditure level (and thus oxygen consumption) calculated as a time-weighted average of metabolic equivalents (METS) over the individual's activities. Many exposure models rely upon EPA's CHAD data base to ...

  8. Conflict and the women of Chad.

    PubMed

    Sy, A D

    1993-06-01

    Chad was used as an example of how women and children were the most affected during national and international conflicts. The conditions of women before and during the conflict were described. Chad has been a country involved in a war provoked by psychological differences between the way of life, economic activity, and culture of Arab Muslims who are nomadic pastoralists, and Animist and Christian farmers. The important year was 1979, when the country was torn apart by civil war. Women before the conflict were part of a patriarchal society of men heading households and sole producers for the family. One decision maker controlled family matters: the man. Men also possessed all household goods. Women were biological and social producers, and value was placed on having large numbers of children. Muslim women lived in seclusion. Married women, who engaged in commercial activity, were mocked and exposed their husbands to the humiliation of not being able to provide sufficiently for the family. Between 1979 and 1982, many men were exiled to other countries nearby, which sometimes meant the abandonment of women and children. The remaining men were limited in their ability to earn money, and reverted to fishing, farming, and hunting. Gender relations changed. Survival strategies were developed, such as women working as domestics or selling products. Petty trading and agricultural and market gardening enterprises were flourishing. Women learned skills in dressmaking, embroidery, or knitting. Sometimes long distances were traveled in order to secure income. Women set up their own banking system with a system of "tontines" or savings cooperatives. The war's impact was to disrupt social and private life, which allowed women to enter the public domain and contribute to economic security. The downside of the conflict was the toll in human life and poverty for vast numbers of women and children. The physical, financial, and psychological exhaustion helped to create an awareness

  9. Cost-estimate and proposal for a development impact bond for canine rabies elimination by mass vaccination in Chad.

    PubMed

    Anyiam, Franziska; Lechenne, Monique; Mindekem, Rolande; Oussigéré, Assandi; Naissengar, Service; Alfaroukh, Idriss Oumar; Mbilo, Celine; Moto, Daugla Doumagoum; Coleman, Paul G; Probst-Hensch, Nicole; Zinsstag, Jakob

    2017-11-01

    Close to 69,000 humans die of rabies each year, most of them in Africa and Asia. Clinical rabies can be prevented by post-exposure prophylaxis (PEP). However, PEP is commonly not available or not affordable in developing countries. Another strategy besides treating exposed humans is the vaccination of vector species. In developing countries, the main vector is the domestic dog, that, once infected, is a serious threat to humans. After a successful mass vaccination of 70% of the dogs in N'Djaména, we report here a cost-estimate for a national rabies elimination campaign for Chad. In a cross-sectional survey in four rural zones, we established the canine : human ratio at the household level. Based on human census data and the prevailing socio-cultural composition of rural zones of Chad, the total canine population was estimated at 1,205,361 dogs (95% Confidence interval 1,128,008-1,736,774 dogs). Cost data were collected from government sources and the recent canine mass vaccination campaign in N'Djaména. A Monte Carlo simulation was used for the simulation of the average cost and its variability, using probability distributions for dog numbers and cost items. Assuming the vaccination of 100 dogs on average per vaccination post and a duration of one year, the total cost for the vaccination of the national Chadian canine population is estimated at 2,716,359 Euros (95% CI 2,417,353-3,035,081) for one vaccination round. A development impact bond (DIB) organizational structure and cash flow scenario were then developed for the elimination of canine rabies in Chad. Cumulative discounted cost of 28.3 million Euros over ten years would be shared between the government of Chad, private investors and institutional donors as outcome funders. In this way, the risk of the investment could be shared and the necessary investment could be made available upfront - a key element for the elimination of canine rabies in Chad. Copyright © 2016 The Authors. Published by Elsevier B

  10. Detection of Tilapia Lake Virus in Clinical Samples by Culturing and Nested Reverse Transcription-PCR

    PubMed Central

    Kembou Tsofack, Japhette Esther; Zamostiano, Rachel; Watted, Salsabeel; Berkowitz, Asaf; Rosenbluth, Ezra; Mishra, Nischay; Briese, Thomas; Lipkin, W. Ian; Kabuusu, Richard M.; Ferguson, Hugh; del Pozo, Jorge

    2016-01-01

    ABSTRACT Tilapia are an important group of farmed fish that serve as a significant protein source worldwide. In recent years, substantial mortality of wild tilapia has been observed in the Sea of Galilee and in commercial ponds in Israel and Ecuador. We have identified the etiological agent of these mass die-offs as a novel orthomyxo-like virus and named it tilapia lake virus (TiLV). Here, we provide the conditions for efficient isolation, culturing, and quantification of the virus, including the use of susceptible fish cell lines. Moreover, we describe a sensitive nested reverse transcription-PCR (RT-PCR) assay allowing the rapid detection of TiLV in fish organs. This assay revealed, for the first time to our knowledge, the presence of TiLV in diseased Colombian tilapia, indicating a wider distribution of this emerging pathogen and stressing the risk that TiLV poses for the global tilapia industry. Overall, the described procedures should provide the tilapia aquaculture industry with important tools for the detection and containment of this pathogen. PMID:27974544

  11. Detection of Tilapia Lake Virus in Clinical Samples by Culturing and Nested Reverse Transcription-PCR.

    PubMed

    Kembou Tsofack, Japhette Esther; Zamostiano, Rachel; Watted, Salsabeel; Berkowitz, Asaf; Rosenbluth, Ezra; Mishra, Nischay; Briese, Thomas; Lipkin, W Ian; Kabuusu, Richard M; Ferguson, Hugh; Del Pozo, Jorge; Eldar, Avi; Bacharach, Eran

    2017-03-01

    Tilapia are an important group of farmed fish that serve as a significant protein source worldwide. In recent years, substantial mortality of wild tilapia has been observed in the Sea of Galilee and in commercial ponds in Israel and Ecuador. We have identified the etiological agent of these mass die-offs as a novel orthomyxo-like virus and named it tilapia lake virus (TiLV). Here, we provide the conditions for efficient isolation, culturing, and quantification of the virus, including the use of susceptible fish cell lines. Moreover, we describe a sensitive nested reverse transcription-PCR (RT-PCR) assay allowing the rapid detection of TiLV in fish organs. This assay revealed, for the first time to our knowledge, the presence of TiLV in diseased Colombian tilapia, indicating a wider distribution of this emerging pathogen and stressing the risk that TiLV poses for the global tilapia industry. Overall, the described procedures should provide the tilapia aquaculture industry with important tools for the detection and containment of this pathogen. Copyright © 2017 American Society for Microbiology.

  12. Pedo-sedimentary constituents as paleoenvironmental proxies in the Sudano-Sahelian belt during the Late Quaternary (southwestern Chad Basin)

    NASA Astrophysics Data System (ADS)

    Diaz, Nathalie; Dietrich, Fabienne; Sebag, David; King, Georgina E.; Valla, Pierre G.; Durand, Alain; Garcin, Yannick; de Saulieu, Geoffroy; Deschamps, Pierre; Herman, Frédéric; Verrecchia, Eric P.

    2018-07-01

    Climate and environmental changes since the Last Glacial Maximum in the tropical zone of West Africa are usually inferred from marine and continental records. In this study, the potential of carbonate pedo-sedimentary geosystems, i.e. Vertisol relics, to record paleoenvironmental changes in the southwestern part of Chad Basin are investigated. A multi-dating approach was applied on different pedogenic organo-mineral constituents. Optically stimulated luminescence (OSL) dating was performed on the soil K-rich feldspars and was combined with radiocarbon dating on both the inorganic (14Cinorg) and organic carbon (14Corg) soil fractions. Three main pedo-sedimentary processes were assessed over the last 20 ka BP: 1) the soil parent material deposition, from 18 ka to 12 ka BP (OSL), 2) the soil organic matter integration, from 11 cal ka to 8 cal ka BP (14Corg), and 3) the pedogenic carbonate nodule precipitation, from 7 cal ka to 5 cal ka BP (14Cinorg). These processes correlate well with the Chad Basin stratigraphy and West African records and are shown to be related to significant changes in the soil water balance responding to the evolution of continental hydrology during the Late Quaternary. The last phase affecting the Vertisol relics is the increase of erosion, which is hypothesized to be due to a decrease of the vegetation cover triggered by (i) the onset of drier conditions, possibly strengthened by (ii) anthropogenic pressure. Archaeological data from Far North Cameroon and northern Nigeria, as well as sedimentation times in Lake Tilla (northeastern Nigeria), were used to test these relationships. The increase of erosion is suggested to possibly occur between c. 3 cal ka and 1 cal ka BP. Finally, satellite images revealed similar geosystems all along the Sudano-Sahelian belt, and initial 14Cinorg ages of the samples collected in four sites gave similar ages to those reported in this study. Consequently, the carbonate pedo-sedimentary geosystems are valuable

  13. The CHADS2 and CHA2DS2-VASc scores for predicting ischemic stroke among East Asian patients with atrial fibrillation: A systemic review and meta-analysis.

    PubMed

    Xiong, Qinmei; Chen, Sisi; Senoo, Keitaro; Proietti, Marco; Hong, Kui; Lip, Gregory Y H

    2015-09-15

    Both the CHADS2 and CHA2DS2-VASc scores are well-validated in Western populations for predicting risk of stroke among patients with atrial fibrillation (AF). There is some uncertainty as to which risk score is best to guide optimal anticoagulant therapy among Asian populations with AF. A systemic literature search of Cochrane library, Scopus, and PubMed databases was conducted using search terms: atrial fibrillation, CHADS2 and CHA2DS2-VASc. Stroke/thromboembolism (TE) outcome events at low, moderate, and high risk groups were compared in relation to both scores. Statistical analyses were performed using Revman 5.3 software. 493 records were retrieved, of which 6 cohort studies focusing on patients from Asian regions were finally appraised and included. Absolute event rates were usually lower when patients were categorized as CHA2DS2-VASc of 0-1, rather than CHADS2 of 0-1, respectively. Meta-analysis revealed that when compared with the CHA2DS2-VASc score, there was a 1.71-fold elevated risk of stroke when patients were stratified as 'low risk' using a CHADS2 score = 0, or a 1.40-fold increase with a CHADS2 score = 1. A 1.19-fold elevated event rate was observed among CHADS2 score ≥ 2 compared to CHA2DS2-VASc, but the total stroke/TE events were numerically higher in patients categorized as CHA2DS2-VASc ≥ 2. The CHA2DS2-VASc score is superior to the CHADS2 score in identifying 'low risk' East Asian AF patients. Rather than a categorical approach, Asian guidelines should adopt a 2 step approach, by initially identifying the truly low risk patients, following which effective stroke prevention can be offered to those with ≥ 1 additional stroke risk factors. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  14. A novel reassortant H3N8 influenza virus isolated from drinking water for duck in a domestic duck farm in Poyang Lake area.

    PubMed

    Dong, Bei Bei; Xu, Cui Ling; Dong, Li Bo; Cheng, Hui Jian; Yang, Lei; Zou, Shu Mei; Chen, Min; Bai, Tian; Zhang, Ye; Gao, Rong Bao; Li, Xiao Dan; Shi, Jing Hong; Yuan, Hui; Yang, Jing; Chen, Tao; Zhu, Yun; Xiong, Ying; Yang, Shuai; Shu, Yue Long

    2013-07-01

    To conduct a full genome sequence analysis for genetic characterization of an H3N8 influenza virus isolated from drinking water of a domestic duck farm in Poyang Lake area in 2011. The virus was cultivated by specific pathogen free (SPF) chicken embryo eggs and was subtyped into hemagglutinin (HA) and neuraminidase (NA) by real-time PCR method. Eight gene segments were sequenced and phylogenetic analysis was conducted. The NA gene of this virus belongs to North American lineage; other seven genes belong to Eurasian lineage. Compared with the viruses containing NA gene, the PB2 and PB1 gene came from different clades. And this indicates that the virus was a novel reassortant genotype. The HA receptor binding preference was avian-like and the cleavage site sequence showed a low pathogenic feature. There was no drug resistance mutation of M2 protein. The mutations of Asn30Asp, and Thr215Ala of the M1 protein implied the potential of pathogenicity increase in mice. The finding of novel genotype of H3N8 virus in drinking water in this duck farm near Poyang Lake highlighted the importance of strengthening the surveillance of avian influenza in this region, which could contribute to pinpointing the influenza ecological relations among avian, swine, and human. Copyright © 2013 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.

  15. Microbial hitchhikers on intercontinental dust: catching a lift in Chad

    PubMed Central

    Favet, Jocelyne; Lapanje, Ales; Giongo, Adriana; Kennedy, Suzanne; Aung, Yin-Yin; Cattaneo, Arlette; Davis-Richardson, Austin G; Brown, Christopher T; Kort, Renate; Brumsack, Hans-Jürgen; Schnetger, Bernhard; Chappell, Adrian; Kroijenga, Jaap; Beck, Andreas; Schwibbert, Karin; Mohamed, Ahmed H; Kirchner, Timothy; de Quadros, Patricia Dorr; Triplett, Eric W; Broughton, William J; Gorbushina, Anna A

    2013-01-01

    Ancient mariners knew that dust whipped up from deserts by strong winds travelled long distances, including over oceans. Satellite remote sensing revealed major dust sources across the Sahara. Indeed, the Bodélé Depression in the Republic of Chad has been called the dustiest place on earth. We analysed desert sand from various locations in Chad and dust that had blown to the Cape Verde Islands. High throughput sequencing techniques combined with classical microbiological methods showed that the samples contained a large variety of microbes well adapted to the harsh desert conditions. The most abundant bacterial groupings in four different phyla included: (a) Firmicutes—Bacillaceae, (b) Actinobacteria—Geodermatophilaceae, Nocardiodaceae and Solirubrobacteraceae, (c) Proteobacteria—Oxalobacteraceae, Rhizobiales and Sphingomonadaceae, and (d) Bacteroidetes—Cytophagaceae. Ascomycota was the overwhelmingly dominant fungal group followed by Basidiomycota and traces of Chytridiomycota, Microsporidia and Glomeromycota. Two freshwater algae (Trebouxiophyceae) were isolated. Most predominant taxa are widely distributed land inhabitants that are common in soil and on the surfaces of plants. Examples include Bradyrhizobium spp. that nodulate and fix nitrogen in Acacia species, the predominant trees of the Sahara as well as Herbaspirillum (Oxalobacteraceae), a group of chemoorganotrophic free-living soil inhabitants that fix nitrogen in association with Gramineae roots. Few pathogenic strains were found, suggesting that African dust is not a large threat to public health. PMID:23254516

  16. Assessing narcolepsy with cataplexy in children and adolescents: development of a cataplexy diary and the ESS-CHAD

    PubMed Central

    Wang, Y Grace; Benmedjahed, Khadra; Lambert, Jérémy; Evans, Christopher J; Hwang, Steve; Black, Jed; Johns, Murray W

    2017-01-01

    Objective The aim of this study was to qualitatively evaluate concepts for incorporation into a daily diary to capture cataplexy frequency and to assess the content validity of the Epworth Sleepiness Scale for Children and Adolescents (ESS-CHAD) in pediatric patients with narcolepsy. Patients and methods Face-to-face concept elicitation and cognitive interviews were conducted with children (7–9 years; n=13) and adolescents (10–17 years; n=16) who have narcolepsy with cataplexy, and their parents/caregivers. Results Similarities and differences were noted between narcolepsy concepts described by children and their parents/caregivers, suggesting some different but complementary perspectives; parents may not recognize cataplexy symptoms/triggers as well as children, but parents have greater recognition of the circumstances of falling asleep. Cataplexy diary modifications included changes in definitions and examples of cataplexy, using child-friendly terminology, adding a quantitative question to determine daily frequency, and standardizing the questionnaire for evening administration with self-completion by the child. Modifications were made to ESS-CHAD for child-friendly wording and to ensure that items reflect activities (eating, watching TV/video) and environments (school, bus/car transport) in which children are likely to participate. Two ESS-CHAD versions were proposed: one with a 1-month recall period, for general use, and the other with a recall period of “since your last study visit,” for research, which could be shorter or longer than 1 month (as short as 1 week). Conclusion The cataplexy diary and ESS-CHAD were modified for the assessment of children and adolescents. Further psychometric validation is recommended. These measures are being used in a Phase III, placebo-controlled clinical trial of sodium oxybate in children and adolescents with narcolepsy. PMID:28860883

  17. Virological surveillance and phylogenetic analysis of the PB2 genes of influenza viruses isolated from wild water birds flying from their nesting lakes in Siberia to Hokkaido, Japan in autumn.

    PubMed

    Samad, Rozanah Asmah Abdul; Sakoda, Yoshihiro; Tsuda, Yoshimi; Simulundu, Edgar; Manzoor, Rashid; Okamatsu, Masatoshi; Ito, Kimihito; Kida, Hiroshi

    2011-02-01

    Recent introduction of H5N1 highly pathogenic avian influenza virus (HPAIV) in wild birds from poultry in Eurasia signaled the possibility that this virus may perpetuate in nature. Surveillance of avian influenza especially in migratory birds, therefore, has been conducted to provide information on the viruses brought by them to Hokkaido, Japan, from their nesting lakes in Siberia in autumn. During 2008-2009, 62 influenza viruses of 21 different combinations of hemagglutinin (HA) and neuraminidase (NA) subtypes were isolated. Up to September 2010, no HPAIV has been found, indicating that H5N1 HPAIV has not perpetuated at least dominantly in the lakes where ducks nest in summer in Siberia. The PB2 genes of 54 influenza viruses out of 283 influenza viruses isolated in Hokkaido in 2000-2009 were phylogenetically analysed. None of the genes showed close relation to those of H5N1 HPAIVs that were detected in wild birds found dead in Eurasia on the way back to their northern territory in spring.

  18. Consolidated Human Activity Database (CHAD) for use in human exposure and health studies and predictive models

    EPA Pesticide Factsheets

    EPA scientists have compiled detailed data on human behavior from 22 separate exposure and time-use studies into CHAD. The database includes more than 54,000 individual study days of detailed human behavior.

  19. Temporal Dynamics and Decay of Putatively Allochthonous and Autochthonous Viral Genotypes in Contrasting Freshwater Lakes

    PubMed Central

    Barbosa, Jorge G.; Brown, Julia M.; Donelan, Ryan P.; Eaglesham, James B.; Eggleston, Erin M.; LaBarre, Brenna A.

    2012-01-01

    Aquatic viruses play important roles in the biogeochemistry and ecology of lacustrine ecosystems; however, their composition, dynamics, and interactions with viruses of terrestrial origin are less extensively studied. We used a viral shotgun metagenomic approach to elucidate candidate autochthonous (i.e., produced within the lake) and allochthonous (i.e., washed in from other habitats) viral genotypes for a comparative study of their dynamics in lake waters. Based on shotgun metagenomes prepared from catchment soil and freshwater samples from two contrasting lakes (Cayuga Lake and Fayetteville Green Lake), we selected two putatively autochthonous viral genotypes (phycodnaviruses likely infecting algae and cyanomyoviruses likely infecting picocyanobacteria) and two putatively allochthonous viral genotypes (geminiviruses likely infecting terrestrial plants and circoviruses infecting unknown hosts but common in soil libraries) for analysis by genotype-specific quantitative PCR (TaqMan) applied to DNAs from viruses in the viral size fraction of lake plankton, i.e., 0.2 μm > virus > 0.02 μm. The abundance of autochthonous genotypes largely reflected expected host abundance, while the abundance of allochthonous genotypes corresponded with rainfall and storm events in the respective catchments, suggesting that viruses with these genotypes may have been transported to the lake in runoff. The decay rates of allochthonous and autochthonous genotypes, assessed in incubations where all potential hosts were killed, were generally lower (0.13 to 1.50% h−1) than those reported for marine virioplankton but similar to those for freshwater virioplankton. Both allochthonous and autochthonous viral genotypes were detected at higher concentrations in subsurface sediments than at the water-sediment interface. Our data indicate that putatively allochthonous viruses are present in lake plankton and sediments, where their temporal dynamics reflect active transport to the lake during

  20. Challenges to implementing Gavi's health system strengthening support in Chad and Cameroon: results from a mixed-methods evaluation.

    PubMed

    Dansereau, Emily; Miangotar, Yodé; Squires, Ellen; Mimche, Honoré; El Bcheraoui, Charbel

    2017-11-16

    Since 2005, Gavi has provided health system strengthening (HSS) grants to address bottlenecks affecting immunization services. This study is the first to evaluate the Gavi HSS implementation process in either Cameroon or Chad, two countries with significant health system challenges and poor achievement on the child and maternal health Millennium Development Goals. We triangulated quantitative and qualitative data including financial records, document review, field visit questionnaires, and key informant interviews (KII) with representatives from the Ministries of Health, Gavi, and other partners. We conducted a Root Cause Analysis of key implementation challenges, guided by the Consolidated Framework for Implementation Research. We conducted 124 field visits and 43 KIIs in Cameroon, and 57 field visits and 39 KIIs in Chad. Cameroon's and Chad's HSS programs were characterized by delayed disbursements, significant deviations from approved expenditures, and reprogramming of funds. Nearly a year after the programs were intended to be complete, many district and facility-level activities were only partially implemented and significant funds remained unabsorbed. Root causes of these challenges included unpredictable Gavi processes and disbursements, poor communication between the countries and Gavi, insufficient country planning without adequate technical assistance, lack of country staff and leadership, and weak country systems to manage finances and promote institutional memory. Though Chad and Cameroon both critically needed support to strengthen their weak health systems, serious challenges drastically limited implementation of their Gavi HSS programs. Implementation of future HSS programs in these and similar settings can be improved by transparent and reliable procedures and communication from Gavi, proposals that account for countries' programmatic capacity and the potential for delayed disbursements, implementation practices that foster learning and adaptation

  1. Is drinking water from 'improved sources' really safe? A case study in the Logone valley (Chad-Cameroon).

    PubMed

    Sorlini, S; Palazzini, D; Mbawala, A; Ngassoum, M B; Collivignarelli, M C

    2013-12-01

    Within a cooperation project coordinated by the Association for Rural Cooperation in Africa and Latin America (ACRA) Foundation, water supplies were sampled across the villages of the Logone valley (Chad-Cameroon) mostly from boreholes, open wells, rivers and lakes as well as from some piped water. Microbiological analyses and sanitary inspections were carried out at each source. The microbiological quality was determined by analysis of indicators of faecal contamination, Escherichia coli, Enterococci and Salmonellae, using the membrane filtration method. Sanitary inspections were done using WHO query forms. The assessment confirmed that there are several parameters of health concern in the studied area; bacteria of faecal origins are the most significant. Furthermore, this study demonstrated that Joint Monitoring Programme (JMP) classification and E. coli measurement are not sufficient to state water safety. In fact, in the studied area, JMP defined 'improved sources' may provide unsafe water depending on their structure and sources without E. coli may have Enterococci and Salmonellae. Sanitary inspections also revealed high health risks for some boreholes. In other cases, sources with low sanitary risk and no E. coli were contaminated by Enterococci and Salmonellae. Better management and protection of the sources, hygiene improvement and domestic water treatment before consumption are possible solutions to reduce health risks in the Logone valley.

  2. Virus-Bacterium Coupling Driven by both Turbidity and Hydrodynamics in an Amazonian Floodplain Lake ▿ † ‡

    PubMed Central

    Barros, Nathan; Farjalla, Vinicius F.; Soares, Maria C.; Melo, Rossana C. N.; Roland, Fábio

    2010-01-01

    The importance of viruses in aquatic ecosystem functioning has been widely described. However, few studies have examined tropical aquatic ecosystems. Here, we evaluated for the first time viruses and their relationship with other planktonic communities in an Amazonian freshwater ecosystem. Coupling between viruses and bacteria was studied, focusing both on hydrologic dynamics and anthropogenic forced turbidity in the system (Lake Batata). Samples were taken during four hydrologic seasons at both natural and impacted sites to count virus-like particles (VLP) and bacteria. In parallel, virus-infected bacteria were identified and quantified by transmission electron microscopy (TEM). Viral abundance ranged from 0.5 × 107 ± 0.2 × 107 VLP ml−1 (high-water season, impacted site) to 1.7 × 107 ± 0.4 × 107 VLP ml−1 (low-water season, natural site). These data were strongly correlated with the bacterial abundance (r2 = 0.84; P < 0.05), which ranged from 1.0 × 106 ± 0.5 × 106 cells ml−1 (high water, impacted site) to 3.4 × 106 ± 0.7 × 106 cells ml−1 (low water, natural site). Moreover, the viral abundance was weakly correlated with chlorophyll a, suggesting that most viruses were bacteriophages. TEM quantitative analyses revealed that the frequency of visibly infected cells was 20%, with 10 ± 3 phages per cell section. In general, we found a low virus-bacterium ratio (<7). Both the close coupling between the viral and bacterial abundances and the low virus-bacterium ratio suggest that viral abundance tends to be driven by the reduction of hosts for viral infection. Our results demonstrate that viruses are controlled by biological substrates, whereas in addition to grazing, bacteria are regulated by physical processes caused by turbidity, which affect underwater light distribution and dissolved organic carbon availability. PMID:20833790

  3. Potential for Waterborne and Invertebrate Transmission of West Nile Virus in the Great Salt Lake, Utah.

    PubMed

    Lund, Melissa; Shearn-Bochsler, Valerie; Dusek, Robert J; Shivers, Jan; Hofmeister, Erik

    2017-07-15

    In November and December of 2013, a large mortality event involving 15,000 to 20,000 eared grebes ( Podiceps nigricollis ) occurred at the Great Salt Lake (GSL), UT. The onset of the outbreak in grebes was followed by a mortality event in >86 bald eagles ( Haliaeetus leucocephalus ). During the die-off, West Nile virus (WNV) was detected by reverse transcription-PCR (RT-PCR) or viral culture in the carcasses of grebes and eagles submitted to the National Wildlife Health Center. However, no activity of mosquitoes, the primary vectors of WNV, was detected by the State of Utah's WNV monitoring program. The transmission of WNV has rarely been reported during the winter in North America in the absence of known mosquito activity; however, the size of this die-off, the habitat in which it occurred, and the species involved are unique. We experimentally investigated whether WNV could survive in water with a high salt content, as found at the GSL, and whether brine shrimp, the primary food of migrating eared grebes on the GSL, could have played a role in the transmission of WNV to feeding birds. We found that WNV can survive up to 72 h at 4°C in water containing 30 to 150 ppt NaCl, and brine shrimp incubated with WNV in 30 ppt NaCl may adsorb WNV to their cuticle and, through feeding, infect epithelial cells of their gut. Both mechanisms may have potentiated the WNV die-off in migrating eared grebes on the GSL. IMPORTANCE Following a major West Nile virus die-off of eared grebes and bald eagles at the Great Salt Lake (GSL), UT, in November to December 2013, this study assessed the survival of West Nile virus (WNV) in water as saline as that of the GSL and whether brine shrimp, the major food for migrating grebes, could have played a role as a vector for the virus. While mosquitoes are the major vector of WNV, under certain circumstances, transmission may occur through contaminated water and invertebrates as food. Copyright © 2017 American Society for Microbiology.

  4. Treatment of human and livestock helminth infections in a mobile pastoralist setting at Lake Chad: Attitudes to health and analysis of active pharmaceutical ingredients of locally available anthelminthic drugs.

    PubMed

    Greter, Helena; Cowan, Noemi; Ngandolo, Bongo N; Kessely, Hamit; Alfaroukh, Idriss O; Utzinger, Jürg; Keiser, Jennifer; Zinsstag, Jakob

    2017-11-01

    Mobile pastoralists face challenges in accessing quality health care and medication for managing human and animal diseases. We determined livestock disease priorities, health seeking behaviour of people bearing helminthiases and - placing particular emphasis on trematode infections - treatment strategies and outcome satisfaction among mobile pastoralists of four ethnic groups in the Lake Chad area using focus group discussions. People suffering from schistosomiasis were interviewed about symptoms, health seeking behaviour and their satisfaction with respect to the provided treatment. Anthelminthic drugs for human and veterinary use obtained from various health care structures were analysed for active pharmaceutical ingredients (API) and quantity, using high pressure liquid chromatography-UV and liquid chromatography combined with tandem mass spectrometry. Most people suffering from schistosomiasis sought treatment at health care centres. Yet, they also consulted informal providers without medical training. Regarding animal health, self-mediated therapy was common to manage suspected livestock fascioliasis. Self-reported treatment satisfaction for human schistosomiasis and trematodiasis treatment outcome in livestock were low. Mobile pastoralists perceived the purchased drugs to be of low quality. Among 33 products locally sold as anthelminthic drugs for human or veterinary use, 27 contained albendazole or mebendazole, varying between 91% and 159% of the labelled amount. Six products were sold loosely with incomplete information and their API could not be identified. No counterfeit anthelminthic drugs were detected. None of the samples contained praziquantel or triclabendazole, the drugs of choice against schistosomiasis and fascioliasis, respectively. The perceived unsatisfactory treatment outcomes in humans and animals infected with trematodes are most likely due to empiric diagnosis and the resulting use of inadequate therapy for human schistosomiasis and the

  5. Choices in the use of ICD-9 codes to identify stroke risk factors can affect the apparent population-level risk factor prevalence and distribution of CHADS2 scores

    PubMed Central

    Rothendler, James A; Rose, Adam J; Reisman, Joel I; Berlowitz, Dan R; Kazis, Lewis E

    2012-01-01

    While developed for managing individuals with atrial fibrillation, risk stratification schemes for stroke, such as CHADS2, may be useful in population-based studies, including those assessing process of care. We investigated how certain decisions in identifying diagnoses from administrative data affect the apparent prevalence of CHADS2-associated diagnoses and distribution of scores. Two sets of ICD-9 codes (more restrictive/ more inclusive) were defined for each CHADS2-associated diagnosis. For stroke/transient ischemic attack (TIA), the more restrictive set was applied to only inpatient data. We varied the number of years (1-3) in searching for relevant codes, and, except for stroke/TIA, the number of instances (1 vs. 2) that diagnoses were required to appear. The impact of choices on apparent disease prevalence varied by type of choice and condition, but was often substantial. Choices resulting in substantial changes in prevalence also tended to be associated with more substantial effects on the distribution of CHADS2 scores. PMID:22937488

  6. Atrial fibrillation incrementally increases dementia risk across all CHADS2 and CHA2DS2VASc strata in patients receiving long-term warfarin.

    PubMed

    Graves, Kevin G; May, Heidi T; Jacobs, Victoria; Bair, Tami L; Stevens, Scott M; Woller, Scott C; Crandall, Brian G; Cutler, Michael J; Day, John D; Mallender, Charles; Osborn, Jeffrey S; Peter Weiss, J; Jared Bunch, T

    2017-06-01

    Patients with atrial fibrillation (AF) are at higher risk for developing dementia. Warfarin is a common therapy for the prevention of thromboembolism in AF, valve replacement, and thrombosis patients. The extent to which AF itself increases dementia risk remains unknown. A total 6030 patients with no history of dementia and chronically anticoagulated with warfarin were studied. Warfarin management was provided through a Clinical Pharmacy Anticoagulation Service. Patients were stratified by warfarin indication of AF (n=3015) and non-AF (n=3015) and matched by propensity score (±0.01). Patients were stratified by the congestive heart failure, hypertension, age >75 years, diabetes, stroke (CHADS 2 ) score calculated at the time of warfarin initiation and followed for incident dementia. The average age of the AF cohort was 69.3±11.2 years, and 52.7% were male; average age of non-AF cohort was 69.3±10.9 years, and 51.5% were male. Increasing CHADS 2 score was associated with increased dementia incidence, P trend=.004. When stratified by warfarin indication, AF patients had an increased risk of dementia incidence. After multivariable adjustment, AF patients continued to display a significantly increased risk of dementia when compared with non-AF patients across all CHADS 2 scores strata. In patients receiving long-term warfarin therapy, dementia risk increased with increasing CHADS 2 scores. However, the presence of AF was associated with higher rates of dementia across all CHADS 2 score strata. These data suggest that AF contributes to the risk of dementia and that this risk is not solely attributable to anticoagulant use. Dementia may be an end manifestation of a systemic disease state, and AF likely contributes to its progression. Copyright © 2017 Elsevier Inc. All rights reserved.

  7. The value of the CHA2DS2-VASc score for refining stroke risk stratification in patients with atrial fibrillation with a CHADS2 score 0-1: a nationwide cohort study.

    PubMed

    Olesen, Jonas Bjerring; Torp-Pedersen, Christian; Hansen, Morten Lock; Lip, Gregory Y H

    2012-06-01

    North American and European guidelines on atrial fibrillation (AF) are conflicting regarding the classification of patients at low/intermediate risk of stroke. We aimed to investigate if the CHA2DS2-VASc score improved risk stratification of AF patients with a CHADS2 score of 0-1. Using individual-level-linkage of nationwide Danish registries 1997-2008, we identified patients discharged with AF having a CHADS2 score of 0-1 and not treated with vitamin K antagonist or heparin. In patients with a CHADS2 score of 0, 1, and 0-1, rates of stroke/ thromboembolism were determined according to CHA2DS2-VASc score, and the risk associated with increasing CHA2DS2-VASc score was estimated in Cox regression models adjusted for year of inclusion and antiplatelet therapy. The value of adding the extra CHA2DS2-VASc risk factors to the CHADS2 score was evaluated by c-statistics, Net Reclassification Improvement (NRI) and Integrated Discrimination Improvement (IDI). We included 47,576 patients with a CHADS2 score of 0-1, from these 7,536 (15.8%) were CHA2DS2-VASc score=0, 10,062 (21.2%) were CHA2DS2-VASc score=1, 14,310 (30.1%) were CHA2DS2-VASc score=2, 14,188 (29.8%) were CHA2DS2-VASc score=3, and 1,480 (3.1%) were CHA2DS2-VASc score=4. Of the cohort with a CHADS2 score of 0-1, the stroke/thromboembolism rate per 100 person-years increased with increasing CHA2DS2-VASc score (95% confidence interval): 0.84 (0.65-1.08), 1.79 (1.53-2.09), 3.67 (3.34-4.03), 5.75 (5.33-6.21), and 8.18 (6.68-10.02) at one year follow-up with CHA2DS2-VASc scores of 0, 1, 2, 3, and 4, respectively. Patients with a CHADS2 score=0 were not all 'low risk', with one-year event rates ranging from 0.84 (CHA2DS2-VASc score=0) to 3.2 (CHA2DS2-VASc score=3). Results from Cox regression analyses, NRI, and IDI confirmed the improved predictive ability of the CHA2DS2-VASc score in the AF patients who have a CHADS2 score of 0-1. In conclusion, the CHA2DS2-VASc provides critical information on risk of stroke in AF

  8. Long-chain 3-hydroxyacyl-coenzyme A dehydrogenase (L-CHAD) deficiency in a patient with the Bannayan-Riley-Ruvalcaba syndrome.

    PubMed

    Fryburg, J S; Pelegano, J P; Bennett, M J; Bebin, E M

    1994-08-01

    Bannayan-Riley-Ruvalcaba syndrome (BRRS) is an autosomal dominant condition of macrocephaly in combination with lipomas/hemangiomas, hypotonia, developmental delay, and a lipid myopathy. The etiology of the lipid storage myopathy has been unclear. We describe a black boy with findings of BRRS who also has a defect in long-chain fatty acid oxidation expressed in cultured skin fibroblasts as a deficiency of long-chain-L-3-hydroxyacyl-CoA dehydrogenase (L-CHAD). He also has an abnormal brain MRI and increased size of both lower limbs. We present this child because of his unusual combination of findings, and postulate that L-CHAD deficiency may be the cause of the lipid myopathy in BRRS.

  9. Comparative susceptibility among three stocks of yellow perch, Perca flavescens (Mitchill), to viral haemorrhagic septicaemia virus strain IVb from the Great Lakes

    USGS Publications Warehouse

    Olson, W.; Emmenegger, E.; Glenn, J.; Winton, J.; Goetz, F.

    2013-01-01

    The Great Lakes strain of viral haemorrhagic septicaemia virus IVb (VHSV-IVb) is capable of infecting a wide number of naive species and has been associated with large fish kills in the Midwestern United States since its discovery in 2005. The yellow perch, Perca flavescens (Mitchill), a freshwater species commonly found throughout inland waters of the United States and prized for its high value in sport and commercial fisheries, is a species documented in several fish kills affiliated with VHS. In the present study, differences in survival after infection with VHSV IVb were observed among juvenile fish from three yellow perch broodstocks that were originally derived from distinct wild populations, suggesting innate differences in susceptibility due to genetic variance. While all three stocks were susceptible upon waterborne exposure to VHS virus infection, fish derived from the Midwest (Lake Winnebago, WI) showed significantly lower cumulative % survival compared with two perch stocks derived from the East Coast (Perquimans River, NC and Choptank River, MD) of the United States. However, despite differences in apparent susceptibility, clinical signs did not vary between stocks and included moderate-to-severe haemorrhages at the pelvic and pectoral fin bases and exophthalmia. After the 28-day challenge was complete, VHS virus was analysed in subsets of whole fish that had either survived or succumbed to the infection using both plaque assay and quantitative PCR methodologies. A direct correlation was identified between the two methods, suggesting the potential for both methods to be used to detect virus in a research setting.

  10. [Computerized temperature monitoring of the vaccine cold chain in a tropical climate, Chad].

    PubMed

    Schlumberger, M; Mireux, F; Tchang, S G; Mboutbogol, D; Cheikh, D O; Hissein, A A; Youssouf, B O; Brahimi, M M; Gamatié, Y

    2011-06-01

    Because new EPI liquid vaccines are highly sensitive to freezing and overheating, close monitoring of the cold chain is mandatory. The new Testostore 171-1 electronic thermometer (Testo) provides more reliable monitoring of cold chain temperature than freezer indicators, vaccine vial monitors and color strips that only indicate if vaccines are out-of-date. The Testo thermometer uses a probe placed in refrigeration units to periodically measure and store temperature readings. Temperature curves are displayed via a USB connection on a laptop computer running special software (Comfort software light). Testo temperature data can easily be communicated to all management levels by e-mail. The first experience using the Testo system in Africa involved regional EPI supervision in Mondou, Logone Occidental, Chad. After a preliminary mission in Chad in 2006 showed the feasibility of using this method to manage the national cold chain at all levels, a nurse was appointed as EPI supervisor and given a refresher course in Chad's capital Ndjamena in March 2009. In April-May 2009, the supervisor was sent back to the Logone Occidental Region to monitor, by himself, refrigeration units making up the regional and district cold chain for vaccine storage in five health centers (rural and urban). Temperature curve readings were performed on site in the presence of the medical staff and results were compared to those recorded twice a day on conventional temperature charts using lamellar thermometers installed in refrigerators doors. Testo curves showed that liquid vaccine storage temperatures fell below freezing too frequently and that temperatures readings of door thermometers were often inaccurate. Testo readings also detected power outages in refrigeration units used in urban settings and flame extinctions in kerosene lamp refrigerators due to refrigerator breakdown or windy weather conditions before the rainy season. The main advantage of this monitoring method is to provide

  11. Great Salt Lake Microbial Communities: The Foundation of a Terminal Lake Ecosystem

    NASA Astrophysics Data System (ADS)

    Baxter, B. K.; Acord, M.; Riddle, M. R.; Avery, B.

    2006-12-01

    Great Salt Lake (GSL) is a natural hypersaline ecosystem and a terminal lake of substantial size. The dramatic fluctuation in water levels and salinity creates an ecological backdrop selective for organisms with a high degree of adaptability. At the macro level, the biodiversity of the GSL ecosystem is simple, due to the limitations of an extreme saline environment: Birds eat the two invertebrates of the lake, and the invertebrates eat phytoplankton. However, analysis of the microbial level reveals an enormous diversity of species interacting with one another and the ecosystem as a whole. Our cultivation, biochemical tests, microscopy and DNA sequencing yielded data on dozens of isolates. These data demonstrate novel species, and possibly genera, living in the lake. In addition, we have discovered viruses (bacteriophage) that prey on the microorganisms. Preliminary data on bacteria dwelling in the gut of the brine shrimp, Artemia franciscana, link these prokaryotic organisms to the food chain for the first time. All of these results taken together open the door for the discussion of the significance of the microbial level of terminal lake ecosystem, particularly in light of lake water contamination and bioremediation possibilities.

  12. Virus-Host and CRISPR Dynamics in Archaea-Dominated Hypersaline Lake Tyrrell, Victoria, Australia

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Emerson, Joanne B.; Andrade, Karen; Thomas, Brian C.

    The study of natural archaeal assemblages requires community context, namely, a concurrent assessment of the dynamics of archaeal, bacterial, and viral populations. Here, we use filter size-resolved metagenomic analyses to report the dynamics of 101 archaeal and bacterial OTUs and 140 viral populations across 17 samples collected over different timescales from 2007–2010 from Australian hypersaline Lake Tyrrell (LT). All samples were dominated by Archaea (75–95%). Archaeal, bacterial, and viral populations were found to be dynamic on timescales of months to years, and different viral assemblages were present in planktonic, relative to host-associated (active and provirus) size fractions. Analyses of clusteredmore » regularly interspaced short palindromic repeat (CRISPR) regions indicate that both rare and abundant viruses were targeted, primarily by lower abundance hosts. Although very few spacers had hits to the NCBI nr database or to the 140 LT viral populations, 21% had hits to unassembled LT viral concentrate reads. This suggests local adaptation to LT-specific viruses and/or undersampling of haloviral assemblages in public databases, along with successful CRISPR-mediated maintenance of viral populations at abundances low enough to preclude genomic assembly. This is the first metagenomic report evaluating widespread archaeal dynamics at the population level on short timescales in a hypersaline system.« less

  13. Virus-Host and CRISPR Dynamics in Archaea-Dominated Hypersaline Lake Tyrrell, Victoria, Australia

    DOE PAGES

    Emerson, Joanne B.; Andrade, Karen; Thomas, Brian C.; ...

    2013-01-01

    The study of natural archaeal assemblages requires community context, namely, a concurrent assessment of the dynamics of archaeal, bacterial, and viral populations. Here, we use filter size-resolved metagenomic analyses to report the dynamics of 101 archaeal and bacterial OTUs and 140 viral populations across 17 samples collected over different timescales from 2007–2010 from Australian hypersaline Lake Tyrrell (LT). All samples were dominated by Archaea (75–95%). Archaeal, bacterial, and viral populations were found to be dynamic on timescales of months to years, and different viral assemblages were present in planktonic, relative to host-associated (active and provirus) size fractions. Analyses of clusteredmore » regularly interspaced short palindromic repeat (CRISPR) regions indicate that both rare and abundant viruses were targeted, primarily by lower abundance hosts. Although very few spacers had hits to the NCBI nr database or to the 140 LT viral populations, 21% had hits to unassembled LT viral concentrate reads. This suggests local adaptation to LT-specific viruses and/or undersampling of haloviral assemblages in public databases, along with successful CRISPR-mediated maintenance of viral populations at abundances low enough to preclude genomic assembly. This is the first metagenomic report evaluating widespread archaeal dynamics at the population level on short timescales in a hypersaline system.« less

  14. Virophage control of antarctic algal host–virus dynamics

    PubMed Central

    Yau, Sheree; Lauro, Federico M.; DeMaere, Matthew Z.; Brown, Mark V.; Thomas, Torsten; Raftery, Mark J.; Andrews-Pfannkoch, Cynthia; Lewis, Matthew; Hoffman, Jeffrey M.; Gibson, John A.; Cavicchioli, Ricardo

    2011-01-01

    Viruses are abundant ubiquitous members of microbial communities and in the marine environment affect population structure and nutrient cycling by infecting and lysing primary producers. Antarctic lakes are microbially dominated ecosystems supporting truncated food webs in which viruses exert a major influence on the microbial loop. Here we report the discovery of a virophage (relative of the recently described Sputnik virophage) that preys on phycodnaviruses that infect prasinophytes (phototrophic algae). By performing metaproteogenomic analysis on samples from Organic Lake, a hypersaline meromictic lake in Antarctica, complete virophage and near-complete phycodnavirus genomes were obtained. By introducing the virophage as an additional predator of a predator–prey dynamic model we determined that the virophage stimulates secondary production through the microbial loop by reducing overall mortality of the host and increasing the frequency of blooms during polar summer light periods. Virophages remained abundant in the lake 2 y later and were represented by populations with a high level of major capsid protein sequence variation (25–100% identity). Virophage signatures were also found in neighboring Ace Lake (in abundance) and in two tropical lakes (hypersaline and fresh), an estuary, and an ocean upwelling site. These findings indicate that virophages regulate host–virus interactions, influence overall carbon flux in Organic Lake, and play previously unrecognized roles in diverse aquatic ecosystems. PMID:21444812

  15. Virophage control of antarctic algal host-virus dynamics.

    PubMed

    Yau, Sheree; Lauro, Federico M; DeMaere, Matthew Z; Brown, Mark V; Thomas, Torsten; Raftery, Mark J; Andrews-Pfannkoch, Cynthia; Lewis, Matthew; Hoffman, Jeffrey M; Gibson, John A; Cavicchioli, Ricardo

    2011-04-12

    Viruses are abundant ubiquitous members of microbial communities and in the marine environment affect population structure and nutrient cycling by infecting and lysing primary producers. Antarctic lakes are microbially dominated ecosystems supporting truncated food webs in which viruses exert a major influence on the microbial loop. Here we report the discovery of a virophage (relative of the recently described Sputnik virophage) that preys on phycodnaviruses that infect prasinophytes (phototrophic algae). By performing metaproteogenomic analysis on samples from Organic Lake, a hypersaline meromictic lake in Antarctica, complete virophage and near-complete phycodnavirus genomes were obtained. By introducing the virophage as an additional predator of a predator-prey dynamic model we determined that the virophage stimulates secondary production through the microbial loop by reducing overall mortality of the host and increasing the frequency of blooms during polar summer light periods. Virophages remained abundant in the lake 2 y later and were represented by populations with a high level of major capsid protein sequence variation (25-100% identity). Virophage signatures were also found in neighboring Ace Lake (in abundance) and in two tropical lakes (hypersaline and fresh), an estuary, and an ocean upwelling site. These findings indicate that virophages regulate host-virus interactions, influence overall carbon flux in Organic Lake, and play previously unrecognized roles in diverse aquatic ecosystems.

  16. Treating Cattle to Protect People? Impact of Footbath Insecticide Treatment on Tsetse Density in Chad

    PubMed Central

    Ndeledje, Noël; Bouyer, Jérémy; Stachurski, Frédéric; Grimaud, Patrice; Belem, Adrien Marie Gaston; Molélé Mbaïndingatoloum, Fidèle; Bengaly, Zakaria; Oumar Alfaroukh, Idriss; Cecchi, Guiliano; Lancelot, Renaud

    2013-01-01

    Background In Chad, several species of tsetse flies (Genus: Glossina ) transmit African animal trypanosomoses (AAT), which represents a major obstacle to cattle rearing, and sleeping sickness, which impacts public health. After the failure of past interventions to eradicate tsetse, the government of Chad is now looking for other approaches that integrate cost-effective intervention techniques, which can be applied by the stake holders to control tsetse-transmitted trypanosomoses in a sustainable manner. The present study thus attempted to assess the efficacy of restricted application of insecticides to cattle leg extremities using footbaths for controlling Glossina m. submorsitans, G . tachinoides and G . f . fuscipes in southern Chad. Methodology/Principal Findings Two sites were included, one close to the historical human African trypanosomiasis (HAT) focus of Moundou and the other to the active foci of Bodo and Moissala. At both sites, a treated and an untreated herd were compared. In the treatment sites, cattle were treated on a regular basis using a formulation of deltamethrin 0.005% (67 to 98 cattle were treated in one of the sites and 88 to 102 in the other one). For each herd, tsetse densities were monthly monitored using 7 biconical traps set along the river and beside the cattle pen from February to December 2009. The impact of footbath treatment on tsetse populations was strong (p < 10-3) with a reduction of 80% in total tsetse catches by the end of the 6-month footbath treatment. Conclusions/Significance The impact of footbath treatment as a vector control tool within an integrated strategy to manage AAT and HAT is discussed in the framework of the “One Health” concept. Like other techniques based on the treatment of cattle, this technology should be used under controlled conditions, in order to avoid the development of insecticide and acaricide resistance in tsetse and tick populations, respectively. PMID:23799148

  17. [Cost estimation of an epidemiological surveillance network for animal diseases in Central Africa: a case study of the Chad network].

    PubMed

    Ouagal, M; Berkvens, D; Hendrikx, P; Fecher-Bourgeois, F; Saegerman, C

    2012-12-01

    In sub-Saharan Africa, most epidemiological surveillance networks for animal diseases were temporarily funded by foreign aid. It should be possible for national public funds to ensure the sustainability of such decision support tools. Taking the epidemiological surveillance network for animal diseases in Chad (REPIMAT) as an example, this study aims to estimate the network's cost by identifying the various costs and expenditures for each level of intervention. The network cost was estimated on the basis of an analysis of the operational organisation of REPIMAT, additional data collected in surveys and interviews with network field workers and a market price listing for Chad. These costs were then compared with those of other epidemiological surveillance networks in West Africa. The study results indicate that REPIMAT costs account for 3% of the State budget allocated to the Ministry of Livestock. In Chad in general, as in other West African countries, fixed costs outweigh variable costs at every level of intervention. The cost of surveillance principally depends on what is needed for surveillance at the local level (monitoring stations) and at the intermediate level (official livestock sectors and regional livestock delegations) and on the cost of the necessary equipment. In African countries, the cost of surveillance per square kilometre depends on livestock density.

  18. Seroepidemiology of Selected Arboviruses in Febrile Patients Visiting Selected Health Facilities in the Lake/River Basin Areas of Lake Baringo, Lake Naivasha, and Tana River, Kenya

    PubMed Central

    Lwande, Olivia; Orindi, Benedict; Irura, Zephania; Ongus, Juliette; Sang, Rosemary

    2015-01-01

    Abstract Introduction: Arboviruses cause emerging and re-emerging infections affecting humans and animals. They are spread primarily by blood-sucking insects such as mosquitoes, ticks, midges, and sandflies. Changes in climate, ecology, demographic, land-use patterns, and increasing global travel have been linked to an upsurge in arboviral disease. Outbreaks occur periodically followed by persistent low-level circulation. Aim: This study was undertaken to determine the seroepidemiology of selected arboviruses among febrile patients in selected lake/river basins of Kenya. Methods: Using a hospital-based cross-sectional descriptive survey, febrile patients were recruited and their serum samples tested for exposure to immunoglobulin M (IgM) and IgG antibodies against Crimean–Congo hemorrhagic fever virus (CCHFV), Rift Valley fever virus (RVFV), West Nile virus (WNV), and chikungunya virus (CHIKV). Samples positive for CHIKV and WNV were further confirmed by the plaque reduction neutralization test (PRNT). Results: Of the 379 samples examined, 176 were IgG positive for at least one of these arboviruses (46.4%, 95% confidence interval [CI] 41.4–51.5%). Virus-specific prevalence for CCHF, RVF, WN, and CHIK was 25.6%, 19.5%, 12.4%, and 2.6%, respectively. These prevalences varied significantly with geographical site (p<0.001), with Tana recording the highest overall arboviral seropositivity. PRNT results for Alphaviruses confirmed that the actual viruses circulating in Baringo were Semliki Forest virus (SFV) and CHIKV, o'nyong nyong virus (ONNV) in Naivasha, and SFV and Sindbis virus (SINDV) in Tana delta. Among the flaviviruses tested, WNV was circulating in all the three sites. Conclusion: There is a high burden of febrile illness in humans due to CCHFV, RVFV, WNV, and CHIKV infection in the river/lake basin regions of Kenya. PMID:25700043

  19. Potential Reservoirs and Risk Factors for VHSV IVb in an Enzootic System: Budd Lake, Michigan.

    PubMed

    Throckmorton, Elizabeth; Brenden, Travis; Peters, Amber K; Newcomb, Tammy J; Whelan, Gary E; Faisal, Mohamed

    2017-03-01

    Viral hemorrhagic septicemia virus genotype IVb (VHSV IVb) has caused major, sporadic fish die-offs in the Laurentian Great Lakes region of North America since 2005. Presently, factors affecting VHSV IVb persistence in enzootic systems are not well understood. Even with annual surveillance, the virus can go undetected for several years after an outbreak before again re-emerging, which suggests that the virus is maintained in the system either below detectable levels or in untested reservoirs. The aim of this study was to identify potential reservoirs of VHSV IVb in Budd Lake, Michigan; VHSV IVb was first detected in Budd Lake in 2007 but remained undetected until 2011. Additionally, we explored the susceptibility of naive fish introduced into a water body enzootic for VHSV IVb by stocking age-0 Largemouth Bass Micropterus salmoides at varying densities into enclosures in the lake. The virus was not detected among samples of the fishes Notropis spp. and Lepomis spp., cylindrical papershell mussels Anodontoides ferussacianus, leeches (subclass Hirudinea), sediment, or water. However, the virus was successfully isolated from amphipods (family Hyalellidae) and Largemouth Bass held in the enclosures. Our finding of VHSV IVb in Hyalellidae amphipods in combination with other research that has detected the virus in Diporeia spp., a large benthic amphipod important as a food resource to Great Lake fishes, suggests that benthic macroinvertebrates are a reservoir for VHSV IVb in infected systems. If there are environmental reservoirs for VHSV IVb in infected systems, they are likely unevenly distributed. Findings of this study add to our understanding of the seemingly complex ecology of this deadly and economically detrimental virus. Received February 22, 2016; accepted October 16, 2016.

  20. Emergence of viral hemorrhagic septicemia virus in the North American Great Lakes region is associated with low viral genetic diversity

    USGS Publications Warehouse

    Thompson, T.M.; Batts, W.N.; Faisal, M.; Bowser, P.; Casey, J.W.; Phillips, K.; Garver, K.A.; Winton, J.; Kurath, G.

    2011-01-01

    Viral hemorrhagic septicemia virus (VHSV) is a fish rhabdovirus that causes disease in a broad range of marine and freshwater hosts. The known geographic range includes the Northern Atlantic and Pacific Oceans, and recently it has invaded the Great Lakes region of North Ame­rica. The goal of this work was to characterize genetic diversity of Great Lakes VHSV isolates at the early stage of this viral emergence by comparing a partial glycoprotein (G) gene sequence (669 nt) of 108 isolates collected from 2003 to 2009 from 31 species and at 37 sites. Phylogenetic analysis showed that all isolates fell into sub-lineage IVb within the major VHSV genetic group IV. Among these 108 isolates, genetic diversity was low, with a maximum of 1.05% within the 669 nt region. There were 11 unique sequences, designated vcG001 to vcG011. Two dominant sequence types, vcG001 and vcG002, accounted for 90% (97 of 108) of the isolates. The vcG001 isolates were most widespread. We saw no apparent association of sequence type with host or year of isolation, but we did note a spatial pattern, in which vcG002 isolates were more prevalent in the easternmost sub-regions, including inland New York state and the St. Lawrence Seaway. Different sequence types were found among isolates from single disease outbreaks, and mixtures of types were evident within 2 isolates from ­individual fish. Overall, the genetic diversity of VHSV in the Great Lakes region was found to be extremely low, consistent with an introduction of a new virus into a geographic region with ­previously naïve host populations.

  1. Emergence of Viral hemorrhagic septicemia virus in the North American Great Lakes region is associated with low viral genetic diversity.

    PubMed

    Thompson, Tarin M; Batts, William N; Faisal, Mohamed; Bowser, Paul; Casey, James W; Phillips, Kenneth; Garver, Kyle A; Winton, James; Kurath, Gael

    2011-08-29

    Viral hemorrhagic septicemia virus (VHSV) is a fish rhabdovirus that causes disease in a broad range of marine and freshwater hosts. The known geographic range includes the Northern Atlantic and Pacific Oceans, and recently it has invaded the Great Lakes region of North America. The goal of this work was to characterize genetic diversity of Great Lakes VHSV isolates at the early stage of this viral emergence by comparing a partial glycoprotein (G) gene sequence (669 nt) of 108 isolates collected from 2003 to 2009 from 31 species and at 37 sites. Phylogenetic analysis showed that all isolates fell into sub-lineage IVb within the major VHSV genetic group IV. Among these 108 isolates, genetic diversity was low, with a maximum of 1.05% within the 669 nt region. There were 11 unique sequences, designated vcG001 to vcG011. Two dominant sequence types, vcG001 and vcG002, accounted for 90% (97 of 108) of the isolates. The vcG001 isolates were most widespread. We saw no apparent association of sequence type with host or year of isolation, but we did note a spatial pattern, in which vcG002 isolates were more prevalent in the easternmost sub-regions, including inland New York state and the St. Lawrence Seaway. Different sequence types were found among isolates from single disease outbreaks, and mixtures of types were evident within 2 isolates from individual fish. Overall, the genetic diversity of VHSV in the Great Lakes region was found to be extremely low, consistent with an introduction of a new virus into a geographic region with previously naive host populations.

  2. Pliocene large-mammal assemblages from northern Chad: sampling and ecological structure

    NASA Astrophysics Data System (ADS)

    Fara, Emmanuel; Likius, Andossa; Mackaye, Hassane T.; Vignaud, Patrick; Brunet, Michel

    2005-11-01

    Numerous Pliocene large-mammal assemblages have been discovered in Chad over the last decade. They offer a unique opportunity to understand the settings in which important chapters of Hominid evolution took place in Central Africa. However, it is crucial to first investigate both sampling and taxonomic homogeneity for these Chadian assemblages because they occur over large sectors in a sandy desert that offers virtually no stratigraphic section. Using cluster analysis and ordination techniques, we show that the three Pliocene sectors from Chad are homogeneous and adequate sampling units. Previous stable isotope analyses on these assemblages have indicated that the environment became richer in C4 plants between approximately 5.3 and 3.5 3 Ma. To test whether this environmental change has affected the structure of palaeo-communities, we assigned body mass, trophic and locomotor eco-variables to mammal species from the three sectors. Statistical analysis shows that the overall ecological structure of the assemblages is not linked with the opening of the plant cover, and eco-variables show no temporal trend from the oldest sector to the youngest. For example, there is no significant change in the relative diversity of grazing and browsing taxa, although mixed feeders are less diversified in the youngest sector than in the preceding one. This pattern apparently does not result from potential biases such as methodological artefacts or taphonomic imprint. Instead, it seems that local heterogeneous environmental factors have played a major role in shaping the ecological spectrum of Chadian mammal palaeo-communities during the Pliocene.

  3. Infectious haematopoietic necrosis virus genogroup-specific virulence mechanisms in sockeye salmon, Oncorhynchus nerka (Walbaum), from Redfish Lake, Idaho

    USGS Publications Warehouse

    Purcell, M.K.; Garver, K.A.; Conway, C.; Elliott, D.G.; Kurath, G.

    2009-01-01

    Characterization of infectious haematopoietic necrosis virus (IHNV) field isolates from North America has established three main genogroups (U, M and L) that differ in host-specific virulence. In sockeye salmon, Oncorhynchus nerka, the U genogroup is highly virulent, whereas the M genogroup is nearly non-pathogenic. In this study, we sought to characterize the virus-host dynamics that contribute to genogroup-specific virulence in a captive stock of sockeye salmon from Redfish Lake in Idaho. Juvenile sockeye salmon were challenged by immersion and injection with either a representative U or M viral strain and sampled periodically until 14 days post-infection (p.i.). Fish challenged with each strain had positive viral titre by day 3, regardless of challenge route, but the fish exposed to the M genogroup virus had significantly lower virus titres than fish exposed to the U genogroup virus. Gene expression analysis by quantitative reverse transcriptase PCR was used to simultaneously assess viral load and host interferon (IFN) response in the anterior kidney. Viral load was significantly higher in the U-challenged fish relative to M-challenged fish. Both viruses induced expression of the IFN-stimulated genes (ISGs), but expression was usually significantly lower in the M-challenged group, particularly at later time points (7 and 14 days p.i.). However, ISG expression was comparable with 3 days post-immersion challenge despite a significant difference in viral load. Our data indicated that the M genogroup virus entered the host, replicated and spread in the sockeye salmon tissues, but to a lesser extent than the U genogroup. Both virus types induced a host IFN response, but the high virulence strain (U) continued to replicate in the presence of this response, whereas the low virulence strain (M) was cleared below detectable levels. We hypothesize that high virulence is associated with early in vivo replication allowing the virus to achieve a threshold level, which the

  4. Infectious haematopoietic necrosis virus genogroup-specific virulence mechanisms in sockeye salmon, Oncorhynchus nerka (Walbaum), from Redfish Lake, Idaho.

    PubMed

    Purcell, M K; Garver, K A; Conway, C; Elliott, D G; Kurath, G

    2009-07-01

    Characterization of infectious haematopoietic necrosis virus (IHNV) field isolates from North America has established three main genogroups (U, M and L) that differ in host-specific virulence. In sockeye salmon, Oncorhynchus nerka, the U genogroup is highly virulent, whereas the M genogroup is nearly non-pathogenic. In this study, we sought to characterize the virus-host dynamics that contribute to genogroup-specific virulence in a captive stock of sockeye salmon from Redfish Lake in Idaho. Juvenile sockeye salmon were challenged by immersion and injection with either a representative U or M viral strain and sampled periodically until 14 days post-infection (p.i.). Fish challenged with each strain had positive viral titre by day 3, regardless of challenge route, but the fish exposed to the M genogroup virus had significantly lower virus titres than fish exposed to the U genogroup virus. Gene expression analysis by quantitative reverse transcriptase PCR was used to simultaneously assess viral load and host interferon (IFN) response in the anterior kidney. Viral load was significantly higher in the U-challenged fish relative to M-challenged fish. Both viruses induced expression of the IFN-stimulated genes (ISGs), but expression was usually significantly lower in the M-challenged group, particularly at later time points (7 and 14 days p.i.). However, ISG expression was comparable with 3 days post-immersion challenge despite a significant difference in viral load. Our data indicated that the M genogroup virus entered the host, replicated and spread in the sockeye salmon tissues, but to a lesser extent than the U genogroup. Both virus types induced a host IFN response, but the high virulence strain (U) continued to replicate in the presence of this response, whereas the low virulence strain (M) was cleared below detectable levels. We hypothesize that high virulence is associated with early in vivo replication allowing the virus to achieve a threshold level, which the

  5. Comparison of CHA2DS2-VASc, CHADS2 and HATCH scores for the prediction of new-onset atrial fibrillation in cancer patients: A nationwide cohort study of 760,339 study participants with competing risk analysis.

    PubMed

    Hu, Wei-Syun; Lin, Cheng-Li

    2017-11-01

    The current study was conducted to assess the ability of CHA 2 DS 2 -VASc, CHADS 2 and HATCH scores in predicting new-onset atrial fibrillation (AF) among patients with cancer. Patients with newly diagnosed cancer between 1 January, 2000 and 31 December, 2011, from the Registry for Catastrophic Illness Patient Database, were defined as the study cohort. CHA 2 DS 2 -VASc, CHADS 2 and HATCH scores were used for new-onset AF prediction in these study patients, and the predictive accuracy of the scores was assessed by the receiver operating characteristics (ROC) curve. A total of 760,339 cancer patients were identified as the study participants. The ROC curves were 0.68 (95% confidence interval [CI] = 0.68-0.69) for the CHA 2 DS 2 -VASc score, 0.67 (95% CI = 0.67-0.68) for the CHADS 2 score and 0.69 (95% CI = 0.69-0.70) for the HATCH score. There were significant differences of c-statistics among CHA 2 DS 2 -VASc score, CHADS 2 score and HATCH score (CHA 2 DS 2 -VASc score vs. CHADS 2 score, p = 0.01; CHA 2 DS 2 -VASc score vs. HATCH score, p = 0.002; CHADS 2 score vs. HATCH score, p < 0.001). The current study is the first to assess the prognostic value of 3 AF risk scores (CHA 2 DS 2 -VASc, CHADS 2 and HATCH scores) in patients with newly-diagnosed cancer. HATCH score was found to have a slightly but significantly better predictive performance than the other 2 scores. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Experimental infection of Tilapia Lake Virus (TiLV) in Nile tilapia (Oreochromis niloticus) and red tilapia (Oreochromis spp.).

    PubMed

    Tattiyapong, Puntanat; Dachavichitlead, Worawan; Surachetpong, Win

    2017-08-01

    Since 2015, a novel orthomyxo-like virus, tilapia lake virus (TiLV) has been associated with outbreaks of disease and massive mortality of cultured Nile and red tilapia (Oreochromis niloticus and Oreochromis spp., respectively) in Thailand. In this study, TiLV was isolated from field samples and propagated in the permissive E-11 cell line, with cytopathic effect (CPE) development within 3-5days post-inoculation. Electron micrographs of infected E-11 cells and fish tissues confirmed the rounded, enveloped virions of 60 to 80nm with characteristics very similar to those of Orthomyxoviridae. In vivo challenge studies showed that high mortality in Nile (86%) and red tilapia (66%) occurred within 4-12days post-infection. The virus was re-isolated from challenged fish tissues in the permissive cell line, and PCR analysis confirmed TiLV as a causative pathogen. The distinct histopathology of challenged fish included massive degeneration and inflammatory cell infiltration in the liver and brain as well as the presence of eosinophilic intracytoplasmic inclusions in hepatocytes and splenic cells. Our results fulfilled Koch's postulates and confirmed that TiLV is an etiologic agent of mass mortality of tilapia in Thailand. The emergence of this virus in many countries has helped increase awareness that it is a potential threat to tilapia aquacultured in Thailand, Asia, and worldwide. Copyright © 2017 Elsevier B.V. All rights reserved.

  7. Use of remote sensing and a geographical information system in a national helminth control programme in Chad.

    PubMed Central

    Brooker, Simon; Beasley, Michael; Ndinaromtan, Montanan; Madjiouroum, Ester Mobele; Baboguel, Marie; Djenguinabe, Elie; Hay, Simon I.; Bundy, Don A. P.

    2002-01-01

    OBJECTIVE: To design and implement a rapid and valid epidemiological assessment of helminths among schoolchildren in Chad using ecological zones defined by remote sensing satellite sensor data and to investigate the environmental limits of helminth distribution. METHODS: Remote sensing proxy environmental data were used to define seven ecological zones in Chad. These were combined with population data in a geographical information system (GIS) in order to define a sampling protocol. On this basis, 20 schools were surveyed. Multilevel analysis, by means of generalized estimating equations to account for clustering at the school level, was used to investigate the relationship between infection patterns and key environmental variables. FINDINGS: In a sample of 1023 schoolchildren, 22.5% were infected with Schistosoma haematobium and 32.7% with hookworm. None were infected with Ascaris lumbricoides or Trichuris trichiura. The prevalence of S. haematobium and hookworm showed marked geographical heterogeneity and the observed patterns showed a close association with the defined ecological zones and significant relationships with environmental variables. These results contribute towards defining the thermal limits of geohelminth species. Predictions of infection prevalence were made for each school surveyed with the aid of models previously developed for Cameroon. These models correctly predicted that A. lumbricoides and T. trichiura would not occur in Chad but the predictions for S. haematobium were less reliable at the school level. CONCLUSION: GIS and remote sensing can play an important part in the rapid planning of helminth control programmes where little information on disease burden is available. Remote sensing prediction models can indicate patterns of geohelminth infection but can only identify potential areas of high risk for S. haematobium. PMID:12471398

  8. A large outbreak of Hepatitis E virus genotype 1 infection in an urban setting in Chad likely linked to household level transmission factors, 2016-2017.

    PubMed

    Spina, Alexander; Lenglet, Annick; Beversluis, David; de Jong, Marja; Vernier, Larissa; Spencer, Craig; Andayi, Fred; Kamau, Charity; Vollmer, Simone; Hogema, Boris; Irwin, Andrea; Ngueremi Yary, Roger; Mahamat Ali, Açyl; Moussa, Ali; Alfani, Prince; Sang, Sibylle

    2017-01-01

    In September 2016, three acutely jaundiced (AJS) pregnant women were admitted to Am Timan Hospital, eastern Chad. We described the outbreak and conducted a case test-negative study to identify risk factors for this genotype of HEV in an acute outbreak setting. Active case finding using a community based surveillance network identified suspected AJS cases. Pregnant or visibly ill AJS cases presenting at hospital were tested with Assure® IgM HEV rapid diagnostic tests (RDTs) and some with Polymerase Chain Reaction (PCR) in Amsterdam; confirmed cases were RDT-positive and controls were RDT-negative. All answered questions around: demographics, household makeup, area of residence, handwashing practices, water collection behaviour and clinical presentation. We calculated unadjusted odds ratios (ORs) and 95% confidence intervals (95% CI). Between September and April 2017, 1443 AJS cases (1293 confirmed) were detected in the town(attack rate: 2%; estimated 65,000 population). PCR testing confirmed HEV genotype 1e. HEV RDTs were used for 250 AJS cases; 100 (40%) were confirmed. Risk factors for HEV infection, included: having at least two children under the age of 5 years (OR 2.1, 95%CI 1.1-4.3), having another household member with jaundice (OR 2.4, 95%CI 0.90-6.3) and, with borderline significance, living in the neighbourhoods of Riad (OR 3.8, 95%CI 1.0-1.8) or Ridina (OR 3.3, 95%CI 1.0-12.6). Cases were more likely to present with vomiting (OR 3.2, 9%CI 1.4-7.9) than controls; possibly due to selection bias. Cases were non-significantly less likely to report always washing hands before meals compared with controls (OR 0.33, 95%CI 0.1-1.1). Our study suggests household factors and area of residence (possibly linked to access to water and sanitation) play a role in HEV transmission; which could inform future outbreak responses. Ongoing sero-prevalence studies will elucidate more aspects of transmission dynamics of this virus with genotype 1e.

  9. Proteins with CHADs (Conserved Histidine α-Helical Domains) Are Attached to Polyphosphate Granules In Vivo and Constitute a Novel Family of Polyphosphate-Associated Proteins (Phosins).

    PubMed

    Tumlirsch, Tony; Jendrossek, Dieter

    2017-04-01

    On the basis of bioinformatic evidence, we suspected that proteins with a CYTH ( Cy aB th iamine triphosphatase) domain and/or a CHAD ( c onserved h istidine α -helical d omain) motif might represent polyphosphate (polyP) granule-associated proteins. We found no evidence of polyP targeting by proteins with CYTH domains. In contrast, two CHAD motif-containing proteins from Ralstonia eutropha H16 (A0104 and B1017) that were expressed as fusions with enhanced yellow fluorescent protein (eYFP) colocalized with polyP granules. While the expression of B1017 was not detectable, the A0104 protein was specifically identified in an isolated polyP granule fraction by proteome analysis. Moreover, eYFP fusions with the CHAD motif-containing proteins MGMSRV2-1987 from Magnetospirillum gryphiswaldense and PP2307 from Pseudomonas putida also colocalized with polyP granules in a transspecies-specific manner. These data indicated that CHAD-containing proteins are generally attached to polyP granules. Together with the findings from four previously polyP-attached proteins (polyP kinases), the results of this study raised the number of polyP-associated proteins in R. eutropha to six. We suggest designating polyP granule-bound proteins with CHAD motifs as phosins ( pho sphate), analogous to pha sins and oleo sins that are specifically bound to the surface of polyhydroxyalkanoate (PHA) granules in PHA-accumulating bacteria and to oil droplets in oil seed plants, respectively. IMPORTANCE The importance of polyphosphate (polyP) for life is evident from the ubiquitous presence of polyP in all species on earth. In unicellular eukaryotic microorganisms, polyP is located in specific membrane-enclosed organelles, called acidocalcisomes. However, in most prokaryotes, polyP is present as insoluble granules that have been designated previously as volutin granules. Almost nothing is known regarding the macromolecular composition of polyP granules. Particularly, the absence or presence of cellular

  10. Groundwater resources of the aquifers of the northern Central African Republic (Ouham Province). First hydrogeological investigations in a changing environment.

    NASA Astrophysics Data System (ADS)

    Djebebe-Ndjiguim, Chantal; Foto, Eric; Backo, Salé; Nguerekossi, Bruno; Zoudamba, Narcisse; Basse-Keke, Eric; Allahdin, Oscar; Huneau, Frédéric; Garel, Emilie; Celle-Jeanton, Hélène; Mabingui, Joseph

    2017-04-01

    Groundwater is a key factor in the socio-economic development of African societies. This is particularly true for the Lake Chad Basin countries for which groundwater is the main water resource for both drinking water supply for population and agriculture, whether small or large scale. The Central African Republic (CAR) occupies a strategic place in the Lake Chad Basin since most waters feeding the different tributaries of the Chari River, which is the main water source of the Lake Chad, are originating from its territory. Indeed, the Northern CAR and particularly the Ouham Province, at the head of the whole Chad endoreic watershed, benefits from favourable rainfall conditions. Unfortunately, very little hydrological and hydrogeological information is available for this area which has never been investigated in terms of geochemical and isotope characterisation. The only available spares technical and scientific investigations over the area are dating from the 1960's. Unfortunately the Lake Chad basin has undergone strong climatological evolutions since the 1970's and hydrological information needs to be updated. The objectives of this study are to characterise groundwater from the Ouham Province in order to better appreciate the hydrogeological processes taking place in the recharge area of the Southern Lake Chad Basin. Isotope hydrology combined with geochemistry of groundwater has now proven being the best approach in under-documented territories to have a first diagnostic on the dynamics and quality of available resources. In this purpose combined hydrogeochemical and isotopic investigations (18O, 2H and 3H of the water molecule) have been launched to constrain groundwater origin, recharge processes, quality, residence time and anthropogenic fingerprint on aquifers. After two sampling campaigns it was possible to draw a general pattern of the hydrogeological and hydrochemical conditions in the region. The Ouham province is mostly composed of Precambrian

  11. Susceptibility of Representative Great Lakes Fish Species to the North Carolina Strain of Spring Viremia of Carp Virus (SVCV).

    PubMed

    Boonthai, Traimat; Loch, Thomas P; Standish, Isaac; Faisal, Mohamed

    2017-12-01

    Spring viremia of carp virus (SVCV) is a notifiable pathogen of the World Organization of Animal Health. Since SVCV was isolated in Lake Ontario in 2007, concern has grown about its spread in the Great Lakes basin and its potential negative impacts on fish species of importance in stock enhancement programs basinwide. The susceptibility of representative fish species from the families Cyprinidae (Fathead Minnow Pimephales promelas, Golden Shiner Notemigonus crysoleucas, Spotfin Shiner Cyprinella spiloptera, and Creek Chub Semotilus atromaculatus), Centrarchidae (Largemouth Bass Micropterus salmoides), Percidae (Walleye Sander vitreus), Salmonidae (Rainbow Trout Oncorhynchus mykiss), and Esocidae (Muskellunge Esox masquinongy) to SVCV was evaluated by experimental infection under laboratory conditions. Morbidity and mortality were recorded, and virus re-isolation, seminested reverse transcription PCR, and histopathological assessments were performed. Using intraperitoneal (i.p.) injection, Fathead Minnows and Golden Shiners were highly susceptible to SVCV (40-70% mortality). All dead or moribund and apparently healthy surviving Fathead Minnows and Golden Shiners were SVCV positive. The SVCV was also detected in challenged but healthy Spotfin Shiners (30%) and Creek Chub (5%). However, noncyprinid species exhibited no morbidity or mortality and were free of SVCV following an observation period of 30 d. In a follow-up experimental challenge, Fathead Minnows and Golden Shiners were SVCV challenged at 10 3 and 10 5 PFU/mL by means of waterborne immersion. After immersion, Fathead Minnows and Golden Shiners exhibited characteristic SVCV disease signs, but mortality was less (30% and 10% mortality, respectively) than that in fish with i.p. injections. The SVCV was detected in all mortalities and a subset of healthy Fathead Minnows and Golden Shiners. Necrotic changes were observed in the kidneys, liver, spleen, ovaries, and heart, and other histopathological lesions also

  12. Development and validation of a reverse transcription quantitative polymerase chain reaction for tilapia lake virus detection in clinical samples and experimentally challenged fish.

    PubMed

    Tattiyapong, P; Sirikanchana, K; Surachetpong, W

    2018-02-01

    Tilapia lake virus (TiLV) is an emerging pathogen associated with high mortalities of wild and farm-raised tilapia in different countries. In this study, a SYBR green-based reverse transcription quantitative polymerase chain reaction (RT-qPCR) assay targeting segment three of the virus was developed to detect and quantify TiLV in clinical samples and experimentally challenged fish. All 30 field samples with clinical signs and history consistent with TiLV infection were positive for TiLV as detected by the developed RT-qPCR method. The RT-qPCR technique provided 100 and 10,000 times more sensitive for virus detection than those offered by the RT-PCR and virus isolation in cell culture methods, respectively. The detection limit of the RT-qPCR method was as low as two viral copies/μl. Moreover, the RT-qPCR technique could be applied for TiLV detection in various fish tissues including gills, liver, brain, heart, anterior kidney and spleen. Significantly, this study delivered an accurate and reliable method for rapid detection of TiLV viruses that facilitates active surveillance programme and disease containment. © 2017 John Wiley & Sons Ltd.

  13. Antibiotic Susceptibility and Molecular Diversity of Bacillus anthracis Strains in Chad: Detection of a New Phylogenetic Subgroup

    PubMed Central

    Maho, Angaya; Rossano, Alexandra; Hächler, Herbert; Holzer, Anita; Schelling, Esther; Zinsstag, Jakob; Hassane, Mahamat H.; Toguebaye, Bhen S.; Akakpo, Ayayi J.; Van Ert, Matthew; Keim, Paul; Kenefic, Leo; Frey, Joachim; Perreten, Vincent

    2006-01-01

    We genotyped 15 Bacillus anthracis isolates from Chad, Africa, using multiple-locus variable-number tandem repeat analysis and three additional direct-repeat markers. We identified two unique genotypes that represent a novel genetic lineage in the A cluster. Chadian isolates were susceptible to 11 antibiotics and free of 94 antibiotic resistance genes. PMID:16954291

  14. Decreased Renal Function Is Associated with Elevated CHA2DS2VASC and R2CHADS2 Scores in Non-Valvular Atrial Fibrillation Patients Presenting with Stroke.

    PubMed

    Vindhyal, Mohinder; Vindhyal, Shravani R; Haneke, Travis; Ndunda, Paul M; Eid, Freidy; Kallail, K James

    2017-12-11

    Introduction Atrial fibrillation (AF), the most common cardiac arrhythmia, affects approximately 2.3 million patients in the United States, costing around $26 billion. Atrial fibrillation is associated with a two- to seven-fold increased risk of stroke, one of the most serious complications. Chronic kidney disease affects approximately 13% of the US population and has been associated with higher rates of AF than the general population. In patients with chronic kidney disease (CKD), the risk of stroke increases as the glomerular filtration rate (GFR) decreases, especially in CKD stages three and four. Several risks stratification scores such as CHADS2 (congestive heart failure, hypertension, age, diabetes mellitus, stroke), CHA2DS2VASc (congestive heart failure, hypertension, age, diabetes mellitus, stroke, vascular disease, age, sex), and R2CHADS2 (renal failure, congestive heart failure, age, diabetes, stroke) scores are used for stroke risk assessment in patients with non-valvular atrial fibrillation (NVAF). This study investigates the association between renal functions and risk stratification scoring systems in patients with non-valvular AF presenting with stroke. Methods Using the convenience sampling method, 171 subjects were selected from the eligible population (n = 386). A Pearson product-moment correlation coefficient was calculated to determine the association between the GFR and each of the CHA2DS2VASc and R2CHADS2 scores. In addition, a Pearson product-moment correlation coefficient was calculated to determine the association between the CHA2DS2VASc and R2CHADS2 scores. Results The selected population represented 44.3% of the eligible subjects. Of these, 88% were Caucasian, 60% were female, and the mean age was 78 years. The mean CHA2DS2VASc score was six (range 2-9). The mean eGFR was 69.77 (range 6-108). Both the mode and the median CHA2DS2VASc score was four (range 2-8). A weak, but significant, negative correlation was found between renal

  15. [Newcastle disease in southern Chad: peak epidemic periods and the impact of vaccination].

    PubMed

    Maho, A; Ndeledje Gondje, N; Mopate, L Y; Ganda Kana, S

    2004-12-01

    In spite of its universally acknowledged importance, backyard chicken production is still being hampered by Newcastle disease in some parts of the world. In Chad, the disease has been reported almost everywhere in the country and confirmed in several regions, but there are no control measures in place. A survey was conducted at three sites in south-eastern Chad in July and August 2001, based on face-to-face interviews with 20% of the peasant farmers keeping chickens at these sites. The aim was to collect information on peak epidemic periods and on ways in which the infection spreads. The survey revealed that the peak epidemic periods for Newcastle disease are April, during the mango harvesting and selling period, and December, when trade increases for the seasonal festivities. The survey also showed that peasant farmers attach great importance to chicken farming. The survey was followed by a vaccination trial in November 2001 and February 2002, using the La Sota strain administered ocularly. All of the birds vaccinated during the trial were successfully protected from the disease and both chicken production and the income of the villagers increased. The authors conclude that in order to sustain poultry farming and maximise production in the southern zone, vaccination programmes must be urgently introduced, campaigns to raise awareness of Newcastle disease should be carried out and financial support to pay for vaccines should be provided. Efforts to combat other causes of poultry mortality must also be undertaken.

  16. Cosmogenic nuclide dating of Sahelanthropus tchadensis and Australopithecus bahrelghazali: Mio-Pliocene hominids from Chad

    PubMed Central

    Lebatard, Anne-Elisabeth; Bourlès, Didier L.; Duringer, Philippe; Jolivet, Marc; Braucher, Régis; Carcaillet, Julien; Schuster, Mathieu; Arnaud, Nicolas; Monié, Patrick; Lihoreau, Fabrice; Likius, Andossa; Mackaye, Hassan Taisso; Vignaud, Patrick; Brunet, Michel

    2008-01-01

    Ages were determined at two hominid localities from the Chad Basin in the Djurab Desert (Northern Chad). In the Koro Toro fossiliferous area, KT 12 locality (16°00′N, 18°53′E) was the site of discovery of Australopithecus bahrelghazali (Abel) and in the Toros-Menalla fossiliferous area, TM 266 locality (16°15′N, 17°29′E) was the site of discovery of Sahelanthropus tchadensis (Toumaï). At both localities, the evolutive degree of the associated fossil mammal assemblages allowed a biochronological estimation of the hominid remains: early Pliocene (3–3.5 Ma) at KT 12 and late Miocene (≈7 Ma) at TM 266. Atmospheric 10Be, a cosmogenic nuclide, was used to quasicontinuously date these sedimentary units. The authigenic 10Be/9Be dating of a pelite relic within the sedimentary level containing Abel yields an age of 3.58 ± 0.27 Ma that points to the contemporaneity of Australopithecus bahrelghazali (Abel) with Australopithecus afarensis (Lucy). The 28 10Be/9Be ages obtained within the anthracotheriid unit containing Toumaï bracket, by absolute dating, the age of Sahelanthropus tchadensis to lie between 6.8 and 7.2 Ma. This chronological constraint is an important cornerstone both for establishing the earliest stages of hominid evolution and for new calibrations of the molecular clock. PMID:18305174

  17. New geochemical data from the Nigerian sector of the Chad basin: implications on hydrocarbon prospectivity

    NASA Astrophysics Data System (ADS)

    Obaje, N. G.; Wehner, H.; Hamza, H.; Scheeder, G.

    2004-04-01

    Organic geochemical studies have been carried out to assess the qualities of source rocks penetrated by four wells (Kemar-1, Murshe-1, Tuma-1 and Ziye-1) in the Nigerian sector of the Chad basin. The Chad basin is a large intracratonic basin in Central West Africa. Commercial hydrocarbon accumulations have been discovered in some sectors of the basin outside the Nigerian border in a structurally related contiguous basin. Fair to poor quality source rocks are inherent in the sequences penetrated by the studied wells. About 80% of all samples have their total organic carbon (TOC) contents more than 0.5 wt.%, the minimum limit for hydrocarbon generation. Juxtaposition of the hydrogen indices against the TOC and Tmax indicates that the source rocks are entirely gas-prone. However, biomarker chromatograms and extract vs. TOC plots indicate the presence of oil shows in Ziye-1 well at a depth of 1210 m. Although generated hydrocarbons (wherever they have accumulated) would be overwhelmingly gaseous, gas is the energy of the future. The gas resources of this part of Nigeria's inland basins can be economically exploited through policies that will increase the tempo of gas-utilization projects and the construction of a national grid of gas pipelines with nodal points of input and output.

  18. Possible illnesses: assessing the health impacts of the Chad Pipeline Project.

    PubMed Central

    Leonard, Lori

    2003-01-01

    Health impact assessments associated with large-scale infrastructure projects, such as the Chad-Cameroon Petroleum Development and Pipeline Project, monitor pre-existing conditions and new diseases associated with particular industries or changes in social organization. This paper suggests that illness self-reports constitute a complementary set of benchmarks to measure the health impacts of these projects, and presents data gathered in ongoing household and health service surveys in Ngalaba, a village near a major oilfield in Chad. In an initial 16-week period of weekly data collection, 363 people reported few of the clinically chronic or asymptomatic conditions expected according to health transition theory, and the overall level of illness reporting was low. Illnesses often were described by symptoms or lay diagnoses. Health care practitioners were consulted rarely; when they were, resources for diagnosis and treatment were limited. Clinically acute, short-duration illnesses (e.g. parasitic infections, toothaches, or hernias) were experienced as chronic conditions and were reported week after week. The low levels of illness reporting and lack of clinically chronic conditions are not taken to mean that rural Chadians are healthy. Rather, the patterns of morbidity reflect a particular local ecology in which health services are organized and care dispensed in ways that limit the possibilities for illness in terms of types of illnesses that can be diagnosed and reported, forms illnesses take, and ways in which illnesses are experienced. Illness self-reports are useful adjuncts to "harder" biological measures in HIAs, particularly in the context of large-scale infrastructure projects with explicit development goals. Rather than providing data on the extent to which harm has been mitigated by corporate, state, and donor activities, self-reports show the possibilities of illness in local contexts. PMID:12894327

  19. Prioritization of prevention activities to combat the spread of HIV/AIDS in resource constrained settings: a cost-effectiveness analysis from Chad, Central Africa.

    PubMed

    Hutton, Guy; Wyss, Kaspar; N'Diékhor, Yemadji

    2003-01-01

    In Chad, as in most sub-Saharan Africa countries, HIV/AIDS poses a massive public health threat as well as an economic burden, with prevalence rates estimated at 9% of the adult population. In defining and readjusting the scope and content of the national HIV/AIDS control activities, policy makers sought to identify the most cost-effective options for HIV/AIDS control. The cost-effectiveness analysis reported in this paper uses a mixture of local and international information sources combined with appropriate assumptions to model the cost-effectiveness of feasible HIV prevention options in Chad, with estimates of the budget impact. The most cost-effective options at under US$100 per infection prevented were peer group education of sex workers and screening of blood donors to identify infected blood before transfusion. These options were followed by mass media and peer group education of high risk men and young people, at around US$500 per infection prevented. Anti-retroviral therapy for HIV infected pregnant women and voluntary counselling and testing were in the order of US$1000 per infection prevented. The paper concludes with recommendations for which activities should be given priority in the next phase of the national HIV/AIDS control programme in Chad.

  20. COMPARISON OF EXERCISE PARTICIPATION RATES FOR CHILDREN IN THE LITERATURE WITH THOSE IN EPA'S CONSOLIDATED HUMAN ACTIVITY DATABASE (CHAD)

    EPA Science Inventory

    CHAD contains over 22,000 person-days of human activity pattern survey data. Part of the database includes exercise participation rates for children 0-17 years old, as well as for adults. Analyses of this database indicates that approximately 34% of the 0-17 age group (herea...

  1. Assessment of undiscovered oil and gas resources of the Chad Basin Province, North-Central Africa

    USGS Publications Warehouse

    Brownfield, Michael E.; Schenk, Christopher J.; Charpentier, Ronald R.; Klett, Timothy R.; Cook, Troy A.; Pollastro, Richard M.; Tennyson, Marilyn E.

    2010-01-01

    The Chad Basin Province located in north-central Africa recently was assessed for undiscovered, technically recoverable oil, natural gas, and natural gas liquids resources as part of the U.S. Geological Survey's (USGS) World Oil and Gas Assessment. Using a geology-based assessment methodology, the USGS estimated mean volumes of 2.32 billion barrels of oil, 14.65 trillion cubic feet of natural gas, and 391 million barrels of natural gas liquids.

  2. Hydrologic, land cover and seasonal patterns of waterborne pathogens in great lakes tributaries

    USDA-ARS?s Scientific Manuscript database

    Great Lakes tributaries deliver waterborne pathogens from a host of sources. To examine the hydrologic, land cover, and seasonal variability of waterborne pathogens, protozoa (2), pathogenic bacteria (4) and human (8) and bovine (8) viruses from eight rivers were monitored in the Great Lakes watersh...

  3. Initial Detection and Molecular Characterization of Namaycush Herpesvirus (Salmonid Herpesvirus 5) in Lake Trout.

    PubMed

    Glenney, Gavin W; Barbash, Patricia A; Coll, John A

    2016-03-01

    A novel herpesvirus was found by molecular methods in samples of Lake Trout Salvelinus namaycush from Lake Erie, Pennsylvania, and Lake Ontario, Keuka Lake, and Lake Otsego, New York. Based on PCR amplification and partial sequencing of polymerase, terminase, and glycoprotein genes, a number of isolates were identified as a novel virus, which we have named Namaycush herpesvirus (NamHV) salmonid herpesvirus 5 (SalHV5). Phylogenetic analyses of three NamHV genes indicated strong clustering with other members of the genus Salmonivirus, placing these isolates into family Alloherpesviridae. The NamHV isolates were identical in the three partially sequenced genes; however, they varied from other salmonid herpesviruses in nucleotide sequence identity. In all three of the genes sequenced, NamHV shared the highest sequence identity with Atlantic Salmon papillomatosis virus (ASPV; SalHV4) isolated from Atlantic Salmon Salmo salar in northern Europe, including northwestern Russia. These results lead one to believe that NamHV and ASPV have a common ancestor that may have made a relatively recent host jump from Atlantic Salmon to Lake Trout or vice versa. Partial nucleotide sequence comparisons between NamHV and ASPV for the polymerase and glycoprotein genes differ by >5% and >10%, respectively. Additional nucleotide sequence comparisons between NamHV and epizootic epitheliotropic disease virus (EEDV/SalHV3) in the terminase, glycoprotein, and polymerase genes differ by >5%, >20%, and >10%, respectively. Thus, NamHV and EEDV may be occupying discrete ecological niches in Lake Trout. Even though NamHV shared the highest genetic identity with ASPV, each of these viruses has a separate host species, which also implies speciation. Additionally, NamHV has been detected over the last 4 years in four separate water bodies across two states, which suggests that NamHV is a distinct, naturally replicating lineage. This, in combination with a divergence in nucleotide sequence from EEDV

  4. The predictive ability of the CHADS2 and CHA2DS2-VASc scores for bleeding risk in atrial fibrillation: the MAQI(2) experience.

    PubMed

    Barnes, Geoffrey D; Gu, Xiaokui; Haymart, Brian; Kline-Rogers, Eva; Almany, Steve; Kozlowski, Jay; Besley, Dennis; Krol, Gregory D; Froehlich, James B; Kaatz, Scott

    2014-08-01

    Guidelines recommend the assessment of stroke and bleeding risk before initiating warfarin anticoagulation in patients with atrial fibrillation. Many of the elements used to predict stroke also overlap with bleeding risk in atrial fibrillation patients and it is tempting to use stroke risk scores to efficiently estimate bleeding risk. Comparison of stroke risk scores to bleeding risk scores to predict bleeding has not been thoroughly assessed. 2600 patients followed at seven anticoagulation clinics were followed from October 2009-May 2013. Five risk models (CHADS2, CHA2DS2-VASc, HEMORR2HAGES, HAS-BLED and ATRIA) were retrospectively applied to each patient. The primary outcome was the first major bleeding event. Area under the ROC curves were compared with C statistic and net reclassification improvement (NRI) analysis was performed. 110 patients experienced a major bleeding event in 2581.6 patient-years (4.5%/year). Mean follow up was 1.0±0.8years. All of the formal bleeding risk scores had a modest predictive value for first major bleeding events (C statistic 0.66-0.69), performing better than CHADS2 and CHA2DS2-VASc scores (C statistic difference 0.10 - 0.16). NRI analysis demonstrated a 52-69% and 47-64% improvement of the formal bleeding risk scores over the CHADS2 score and CHA2DS2-VASc score, respectively. The CHADS2 and CHA2DS2-VASc scores did not perform as well as formal bleeding risk scores for prediction of major bleeding in non-valvular atrial fibrillation patients treated with warfarin. All three bleeding risk scores (HAS-BLED, ATRIA and HEMORR2HAGES) performed moderately well. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Genome of Phaeocystis globosa virus PgV-16T highlights the common ancestry of the largest known DNA viruses infecting eukaryotes

    PubMed Central

    Santini, Sebastien; Jeudy, Sandra; Bartoli, Julia; Poirot, Olivier; Lescot, Magali; Abergel, Chantal; Barbe, Valérie; Wommack, K. Eric; Noordeloos, Anna A. M.; Brussaard, Corina P. D.; Claverie, Jean-Michel

    2013-01-01

    Large dsDNA viruses are involved in the population control of many globally distributed species of eukaryotic phytoplankton and have a prominent role in bloom termination. The genus Phaeocystis (Haptophyta, Prymnesiophyceae) includes several high-biomass-forming phytoplankton species, such as Phaeocystis globosa, the blooms of which occur mostly in the coastal zone of the North Atlantic and the North Sea. Here, we report the 459,984-bp-long genome sequence of P. globosa virus strain PgV-16T, encoding 434 proteins and eight tRNAs and, thus, the largest fully sequenced genome to date among viruses infecting algae. Surprisingly, PgV-16T exhibits no phylogenetic affinity with other viruses infecting microalgae (e.g., phycodnaviruses), including those infecting Emiliania huxleyi, another ubiquitous bloom-forming haptophyte. Rather, PgV-16T belongs to an emerging clade (the Megaviridae) clustering the viruses endowed with the largest known genomes, including Megavirus, Mimivirus (both infecting acanthamoeba), and a virus infecting the marine microflagellate grazer Cafeteria roenbergensis. Seventy-five percent of the best matches of PgV-16T–predicted proteins correspond to two viruses [Organic Lake phycodnavirus (OLPV)1 and OLPV2] from a hypersaline lake in Antarctica (Organic Lake), the hosts of which are unknown. As for OLPVs and other Megaviridae, the PgV-16T sequence data revealed the presence of a virophage-like genome. However, no virophage particle was detected in infected P. globosa cultures. The presence of many genes found only in Megaviridae in its genome and the presence of an associated virophage strongly suggest that PgV-16T shares a common ancestry with the largest known dsDNA viruses, the host range of which already encompasses the earliest diverging branches of domain Eukarya. PMID:23754393

  6. Proposal for a revised taxonomy of the family Filoviridae: classification, names of taxa and viruses, and virus abbreviations

    PubMed Central

    Kuhn, Jens H.; Becker, Stephan; Ebihara, Hideki; Geisbert, Thomas W.; Johnson, Karl M.; Kawaoka, Yoshihiro; Lipkin, W. Ian; Negredo, Ana I.; Netesov, Sergey V.; Nichol, Stuart T.; Palacios, Gustavo; Peters, Clarence J.; Tenorio, Antonio; Volchkov, Viktor E.; Jahrling, Peter B.

    2011-01-01

    The taxonomy of the family Filoviridae (marburgviruses and ebolaviruses) has changed several times since the discovery of its members, resulting in a plethora of species and virus names and abbreviations. The current taxonomy has only been partially accepted by most laboratory virologists. Confusion likely arose for several reasons: species names that consist of several words or which (should) contain diacritical marks, the current orthographic identity of species and virus names, and the similar pronunciation of several virus abbreviations in the absence of guidance for the correct use of vernacular names. To rectify this problem, we suggest (1) to retain the current species names Reston ebolavirus, Sudan ebolavirus, and Zaire ebolavirus, but to replace the name Cote d'Ivoire ebolavirus [sic] with Taï Forest ebolavirus and Lake Victoria marburgvirus with Marburg marburgvirus; (2) to revert the virus names of the type marburgviruses and ebolaviruses to those used for decades in the field (Marburg virus instead of Lake Victoria marburgvirus and Ebola virus instead of Zaire ebolavirus); (3) to introduce names for the remaining viruses reminiscent of jargon used by laboratory virologists but nevertheless different from species names (Reston virus, Sudan virus, Taï Forest virus), and (4) to introduce distinct abbreviations for the individual viruses (RESTV for Reston virus, SUDV for Sudan virus, and TAFV for Taï Forest virus), while retaining that for Marburg virus (MARV) and reintroducing that used over decades for Ebola virus (EBOV). Paying tribute to developments in the field, we propose (a) to create a new ebolavirus species (Bundibugyo ebolavirus) for one member virus (Bundibugyo virus, BDBV); (b) to assign a second virus to the species Marburg marburgvirus (Ravn virus, RAVV) for better reflection of now available high-resolution phylogeny; and (c) to create a new tentative genus (Cuevavirus) with one tentative species (Lloviu cuevavirus) for the recently

  7. Proposal for a revised taxonomy of the family Filoviridae: classification, names of taxa and viruses, and virus abbreviations.

    PubMed

    Kuhn, Jens H; Becker, Stephan; Ebihara, Hideki; Geisbert, Thomas W; Johnson, Karl M; Kawaoka, Yoshihiro; Lipkin, W Ian; Negredo, Ana I; Netesov, Sergey V; Nichol, Stuart T; Palacios, Gustavo; Peters, Clarence J; Tenorio, Antonio; Volchkov, Viktor E; Jahrling, Peter B

    2010-12-01

    The taxonomy of the family Filoviridae (marburgviruses and ebolaviruses) has changed several times since the discovery of its members, resulting in a plethora of species and virus names and abbreviations. The current taxonomy has only been partially accepted by most laboratory virologists. Confusion likely arose for several reasons: species names that consist of several words or which (should) contain diacritical marks, the current orthographic identity of species and virus names, and the similar pronunciation of several virus abbreviations in the absence of guidance for the correct use of vernacular names. To rectify this problem, we suggest (1) to retain the current species names Reston ebolavirus, Sudan ebolavirus, and Zaire ebolavirus, but to replace the name Cote d'Ivoire ebolavirus [sic] with Taï Forest ebolavirus and Lake Victoria marburgvirus with Marburg marburgvirus; (2) to revert the virus names of the type marburgviruses and ebolaviruses to those used for decades in the field (Marburg virus instead of Lake Victoria marburgvirus and Ebola virus instead of Zaire ebolavirus); (3) to introduce names for the remaining viruses reminiscent of jargon used by laboratory virologists but nevertheless different from species names (Reston virus, Sudan virus, Taï Forest virus), and (4) to introduce distinct abbreviations for the individual viruses (RESTV for Reston virus, SUDV for Sudan virus, and TAFV for Taï Forest virus), while retaining that for Marburg virus (MARV) and reintroducing that used over decades for Ebola virus (EBOV). Paying tribute to developments in the field, we propose (a) to create a new ebolavirus species (Bundibugyo ebolavirus) for one member virus (Bundibugyo virus, BDBV); (b) to assign a second virus to the species Marburg marburgvirus (Ravn virus, RAVV) for better reflection of now available high-resolution phylogeny; and (c) to create a new tentative genus (Cuevavirus) with one tentative species (Lloviu cuevavirus) for the recently

  8. A third generation vaccine for human visceral leishmaniasis and post kala azar dermal leishmaniasis: First-in-human trial of ChAd63-KH

    PubMed Central

    Osman, Mohamed; Mistry, Anoop; Keding, Ada; Cook, Elizabeth; Wiggins, Rebecca; Di Marco, Stefania; Colloca, Stefano; Siani, Loredana; Smith, Deborah F.; Aebischer, Toni; Lacey, Charles J.

    2017-01-01

    Background Visceral leishmaniasis (VL or kala azar) is the most serious form of human leishmaniasis, responsible for over 20,000 deaths annually, and post kala azar dermal leishmaniasis (PKDL) is a stigmatizing skin condition that often occurs in patients after successful treatment for VL. Lack of effective or appropriately targeted cell mediated immunity, including CD8+ T cell responses, underlies the progression of VL and progression to PKDL, and can limit the therapeutic efficacy of anti-leishmanial drugs. Hence, in addition to the need for prophylactic vaccines against leishmaniasis, the development of therapeutic vaccines for use alone or in combined immuno-chemotherapy has been identified as an unmet clinical need. Here, we report the first clinical trial of a third-generation leishmaniasis vaccine, developed intentionally to induce Leishmania-specific CD8+ T cells. Methods We conducted a first-in-human dose escalation Phase I trial in 20 healthy volunteers to assess the safety, tolerability and immunogenicity of a prime-only adenoviral vaccine for human VL and PKDL. ChAd63-KH is a replication defective simian adenovirus expressing a novel synthetic gene (KH) encoding two Leishmania proteins KMP-11 and HASPB. Uniquely, the latter was engineered to reflect repeat domain polymorphisms and arrangements identified from clinical isolates. We monitored innate immune responses by whole blood RNA-Seq and antigen specific CD8+ T cell responses by IFNγ ELISPOT and intracellular flow cytometry. Findings ChAd63-KH was safe at intramuscular doses of 1x1010 and 7.5x1010 vp. Whole blood transcriptomic profiling indicated that ChAd63-KH induced innate immune responses characterized by an interferon signature and the presence of activated dendritic cells. Broad and quantitatively robust CD8+ T cell responses were induced by vaccination in 100% (20/20) of vaccinated subjects. Conclusion The results of this study support the further development of ChAd63-KH as a novel third

  9. A third generation vaccine for human visceral leishmaniasis and post kala azar dermal leishmaniasis: First-in-human trial of ChAd63-KH.

    PubMed

    Osman, Mohamed; Mistry, Anoop; Keding, Ada; Gabe, Rhian; Cook, Elizabeth; Forrester, Sarah; Wiggins, Rebecca; Di Marco, Stefania; Colloca, Stefano; Siani, Loredana; Cortese, Riccardo; Smith, Deborah F; Aebischer, Toni; Kaye, Paul M; Lacey, Charles J

    2017-05-01

    Visceral leishmaniasis (VL or kala azar) is the most serious form of human leishmaniasis, responsible for over 20,000 deaths annually, and post kala azar dermal leishmaniasis (PKDL) is a stigmatizing skin condition that often occurs in patients after successful treatment for VL. Lack of effective or appropriately targeted cell mediated immunity, including CD8+ T cell responses, underlies the progression of VL and progression to PKDL, and can limit the therapeutic efficacy of anti-leishmanial drugs. Hence, in addition to the need for prophylactic vaccines against leishmaniasis, the development of therapeutic vaccines for use alone or in combined immuno-chemotherapy has been identified as an unmet clinical need. Here, we report the first clinical trial of a third-generation leishmaniasis vaccine, developed intentionally to induce Leishmania-specific CD8+ T cells. We conducted a first-in-human dose escalation Phase I trial in 20 healthy volunteers to assess the safety, tolerability and immunogenicity of a prime-only adenoviral vaccine for human VL and PKDL. ChAd63-KH is a replication defective simian adenovirus expressing a novel synthetic gene (KH) encoding two Leishmania proteins KMP-11 and HASPB. Uniquely, the latter was engineered to reflect repeat domain polymorphisms and arrangements identified from clinical isolates. We monitored innate immune responses by whole blood RNA-Seq and antigen specific CD8+ T cell responses by IFNγ ELISPOT and intracellular flow cytometry. ChAd63-KH was safe at intramuscular doses of 1x1010 and 7.5x1010 vp. Whole blood transcriptomic profiling indicated that ChAd63-KH induced innate immune responses characterized by an interferon signature and the presence of activated dendritic cells. Broad and quantitatively robust CD8+ T cell responses were induced by vaccination in 100% (20/20) of vaccinated subjects. The results of this study support the further development of ChAd63-KH as a novel third generation vaccine for VL and PKDL. This

  10. [Contents of macromineral and trace elements in spirulina (Arthrospira platensis) from France, Chad, Togo, Niger, Mali, Burkina-Faso and Central African Republic].

    PubMed

    Vicat, Jean-Paul; Doumnang Mbaigane, Jean-Claude; Bellion, Yves

    2014-01-01

    Data on mineral elements in spirulinas being limited, we analyzed macrominerals and trace elements of samples from France and Africa. Spirulinas cultivated in France have a composition in macromineral elements similar to those of the literature. The entire contents of trace elements are low. Unlike marine cyanobacteria, they do not concentrate rare-earth elements. Spirulina harvested in Chad has high levels in macrominerals and trace elements, due to traditional drying and harvesting methods. Rare-earth element levels are attributed to this pollution and not to their concentration in spirulinas, because rare-earth element normalized profiles of spirulina are strictly parallel to those of ouadis mud and very different from those of ouadis water. Despite the sometimes high content of total As, normal water consumption in Chad presents no health problems. Spirulinas grown in Togo, Niger, Mali, Burkina-Faso and Central African Republic have chemical compositions similar to those of Chad spirulinas, but with a lower content of macromineral and trace elements, reflecting a lower mineral pollution. Rare-earth element normalized patterns dismiss an aeolian pollution and the pollution is rather of pedological origin. They show no toxicity problem except spirulinas from Burkina-Faso, whose Pb content is too high. The variability of composition of spirulinas can be largely attributed to the mineral pollution of the samples. Significant levels of rare-earth elements sometimes found in the literature reflect this pollution. Copyright © 2013 Académie des sciences. Published by Elsevier SAS. All rights reserved.

  11. Perceptions of the usefulness of external support to immunization coverage in Chad: an analysis of the GAVI-Alliance cash-based support.

    PubMed

    Ferrinho, Paulo; Dramé, Mohammed; Tumusiime, Prosper

    2013-01-01

    Chad is one of the countries supported by the GAVI-Alliance that remains with unsatisfactory vaccination coverage. This paper tries to understand the main barriers to better coverage. These barriers were categorised as up or downstream against the health system building blocks as proposed by WHO and compared with barriers and activities identified by the country in its health system's strengthening grant proposal as approved by the GAVI Alliance in 2007. Data were collected using a modified Delphi system and by analysis of grant and annual report documents. Most of the activities anticipated under the GAVI health system's strengthening proposal are activities targeting downstream barriers (the neglect of upstream issues is of major importance in a decentralised state like Chad) and aligned with, not complementary to, immunization services strengthening activities. Further, both set of cash grants are blind to important recommendations such as the need to address barriers at the level of leadership and governance and at the level of the financing system and also about initiatives to promote community demand of vaccination services. IN CHAD SLOW VACCINATION PROGRESS IS AGGRAVATED BY SEVERAL CONTEXTUAL BARRIERS: the size of the country, the low population density, the nomadic nature of a significant part of its peoples, the recent civil war, associated with civil unrest and political instability and its geographical localization. In this situation it would be important to sustain downstream operations (the major focus of the ISS grant) while taking a long term view of the needs of the health system. The GAVI effectively supports downstream operations, but neglects the long term view.

  12. Composition and cytotoxic activity of essential oils from Xylopia aethiopica (Dunal) A. Rich, Xylopia parviflora (A. Rich) Benth.) and Monodora myristica (Gaertn) growing in Chad and Cameroon.

    PubMed

    Bakarnga-Via, Issakou; Hzounda, Jean Baptiste; Fokou, Patrick Valere Tsouh; Tchokouaha, Lauve Rachel Yamthe; Gary-Bobo, Magali; Gallud, Audrey; Garcia, Marcel; Walbadet, Lucain; Secka, Youssouf; Dongmo, Pierre Michel Jazet; Boyom, Fabrice Fekam; Menut, Chantal

    2014-04-04

    Cancer has become a global public health problem and the search for new control measures is urgent. Investigation of plant products such as essential oils from Monodora myristica, Xylopia aethiopica and Xylopia parviflora might lead to new anticancer therapy. In this study, we have investigated the antineoplastic activity of essential oils from fruits of these plants growing in Chad and Cameroon. The essential oils obtained by hydrodistillation of fruits of Monodora myristica, Xylopia aethiopica and Xylopia parviflora collected in Chad and Cameroon were analyzed by GC-FID and GC-MS and investigated for their antiproliferative activity against the breast cancer cell line (MCF7). Overall, monoterpenes were mostly found in the six essential oils. Oils from X. aethiopica and X. parviflora from Chad and Cameroon mainly contain β-pinene at 24.6%, 28.2%, 35.7% and 32.9% respectively. Monodora myristica oils from both origins contain mainly α-phellandrene at 52.7% and 67.1% respectively. The plant origin did not significantly influence the chemical composition of oils. The six essential oils exerted cytotoxic activity against cancer (MCF-7) and normal cell lines (ARPE-19), with more pronounced effect on neoplastic cells in the majority of cases. The highest selectivity was obtained with the essential oils of X. parviflora from Chad and Cameroon (5.87 and 5.54) which were more cytotoxic against MCF-7 than against normal cell line (ARPE-19) with IC50 values of 0.155 μL/mL and 0.166 μL/mL respectively. Essential oils from fruits of Monodora myristica, Xylopia aethiopica and Xylopia parviflora have shown acceptable antineoplastic potency, and might be investigated further in this regard.

  13. Detection of Viral Hemorrhagic Septicemia virus (VHSV) from the leech Myzobdella lugubris Leidy, 1851

    PubMed Central

    Faisal, Mohamed; Schulz, Carolyn A

    2009-01-01

    The leech Myzobdella lugubris is widespread in the Lake Erie Watershed, especially Lake St. Clair. However, its role in pathogen transmission is not fully understood. In this same watershed, several widespread fish mortalities associated with the Viral Hemorrhagic Septicemia virus (VHSV) were recorded. Viral Hemorrhagic Septicemia is an emerging disease in the Great Lakes Basin that is deadly to the fish population, yet little is known about its mode of transmission. To assess the potential role of M. lugubris in VHSV transmission, leeches were collected from Lake St. Clair and Lake Erie and pooled into samples of five. Cell culture and reverse transcriptase polymerase chain reaction (RT-PCR) were used to determine the presence of the virus and its identity. Results showed that 57 of the 91 pooled leech samples were positive by cell culture for VHSV and 66 of the 91 pooled leech samples were positive by RT-PCR for the VHSV. Two representative virus isolates were sequenced for further genetic confirmation and genotype classification. VHSV detected within M. lugubris was homologous to the Great Lakes strain of VHSV genotype IVb. This is the first record of the VHSV being detected from within a leech, specifically M. lugubris, and suggests the potential of M. lugubris being involved in VHSV transmission. PMID:19785752

  14. Migration of Chadic speaking pastoralists within Africa based on population structure of Chad Basin and phylogeography of mitochondrial L3f haplogroup.

    PubMed

    Cerný, Viktor; Fernandes, Verónica; Costa, Marta D; Hájek, Martin; Mulligan, Connie J; Pereira, Luísa

    2009-03-23

    Chad Basin, lying within the bidirectional corridor of African Sahel, is one of the most populated places in Sub-Saharan Africa today. The origin of its settlement appears connected with Holocene climatic ameliorations (aquatic resources) that started ~10,000 years before present (YBP). Although both Nilo-Saharan and Niger-Congo language families are encountered here, the most diversified group is the Chadic branch belonging to the Afro-Asiatic language phylum. In this article, we investigate the proposed ancient migration of Chadic pastoralists from Eastern Africa based on linguistic data and test for genetic traces of this migration in extant Chadic speaking populations. We performed whole mitochondrial genome sequencing of 16 L3f haplotypes, focused on clade L3f3 that occurs almost exclusively in Chadic speaking people living in the Chad Basin. These data supported the reconstruction of a L3f phylogenetic tree and calculation of times to the most recent common ancestor for all internal clades. A date ~8,000 YBP was estimated for the L3f3 sub-haplogroup, which is in good agreement with the supposed migration of Chadic speaking pastoralists and their linguistic differentiation from other Afro-Asiatic groups of East Africa. As a whole, the Afro-Asiatic language family presents low population structure, as 92.4% of mtDNA variation is found within populations and only 3.4% of variation can be attributed to diversity among language branches. The Chadic speaking populations form a relatively homogenous cluster, exhibiting lower diversification than the other Afro-Asiatic branches (Berber, Semitic and Cushitic). The results of our study support an East African origin of mitochondrial L3f3 clade that is present almost exclusively within Chadic speaking people living in Chad Basin. Whole genome sequence-based dates show that the ancestral haplogroup L3f must have emerged soon after the Out-of-Africa migration (around 57,100 +/- 9,400 YBP), but the "Chadic" L3f3 clade has

  15. The association between different molecular weights of hyaluronic acid and CHAD, HIF-1α, COL2A1 expression in chondrocyte cultures

    PubMed Central

    Sirin, Duygu Yasar; Kaplan, Necati; Yilmaz, Ibrahim; Karaarslan, Numan; Ozbek, Hanefi; Akyuva, Yener; Kaya, Yasin Emre; Oznam, Kadir; Akkaya, Nuray; Guler, Olcay; Akkaya, Semih; Mahirogullari, Mahir

    2018-01-01

    The aim of the present study was to investigate the effects of three different formulations of hyaluronic acid (HA): Low molecular weight (MW) Sinovial One®, medium MW Viscoplus® and high MW Durolane®, on chondrocyte proliferation and collagen type II (COL2A1), hypoxia-inducible factor 1α (HIF-1α) and chondroadherin (CHAD) expression in primary chondrocyte cultures. Standard primary chondrocyte cultures were established from osteochondral tissues surgically obtained from 6 patients with gonarthrosis. Cell morphology was evaluated using an inverted light microscope; cell proliferation was determined with a MTT assay and confirmed with acridine orange/propidium iodide staining. Levels of CHAD, COL2A1 and HIF-1α expression were assessed using specific TaqMan gene expression assays. The results demonstrated the positive effect of HA treatment on cell proliferation, which was independent from the MW. COL2A1 expression increased in the medium and high MW HA treated groups. It was observed that HIF-1α expression increased in the high MW treated group alone. CHAD expression increased only in the medium MW HA treated group. Evaluation of gene expression revealed that levels of expression increased as the duration of HA application increased, in the medium and high MW HA treated groups. In terms of increased viability and proliferation, a longer duration of HA application was more effective. Taken together, it may be concluded that the administration of medium and high MW HA may be a successful way of treating diseases affecting chondrocytes in a clinical setting. PMID:29849772

  16. Perceptions of the usefulness of external support to immunization coverage in Chad: an analysis of the GAVI-Alliance cash-based support

    PubMed Central

    Ferrinho, Paulo; Dramé, Mohammed; Tumusiime, Prosper

    2013-01-01

    Introduction Chad is one of the countries supported by the GAVI-Alliance that remains with unsatisfactory vaccination coverage. This paper tries to understand the main barriers to better coverage. Methods These barriers were categorised as up or downstream against the health system building blocks as proposed by WHO and compared with barriers and activities identified by the country in its health system's strengthening grant proposal as approved by the GAVI Alliance in 2007. Data were collected using a modified Delphi system and by analysis of grant and annual report documents. Results Most of the activities anticipated under the GAVI health system's strengthening proposal are activities targeting downstream barriers (the neglect of upstream issues is of major importance in a decentralised state like Chad) and aligned with, not complementary to, immunization services strengthening activities. Further, both set of cash grants are blind to important recommendations such as the need to address barriers at the level of leadership and governance and at the level of the financing system and also about initiatives to promote community demand of vaccination services. Conclusion In Chad slow vaccination progress is aggravated by several contextual barriers: the size of the country, the low population density, the nomadic nature of a significant part of its peoples, the recent civil war, associated with civil unrest and political instability and its geographical localization. In this situation it would be important to sustain downstream operations (the major focus of the ISS grant) while taking a long term view of the needs of the health system. The GAVI effectively supports downstream operations, but neglects the long term view. PMID:24106572

  17. Antithrombotic treatment and the risk of death and stroke in patients with atrial fibrillation and a CHADS2 score=1.

    PubMed

    Gorin, Laurent; Fauchier, Laurent; Nonin, Emilie; de Labriolle, Axel; Haguenoer, Ken; Cosnay, Pierre; Babuty, Dominique; Charbonnier, Bernard

    2010-04-01

    In patients with atrial fibrillation (AF) and an intermediate risk of stroke (CHADS2 score =1), available evidence from clinical trials is inconclusive and the present guidelines for the management of AF indicate that the choice between oral anticoagulant and aspirin in these patients is open. Our goal was to evaluate whether, in patients with AF and only one moderate risk factor for thromboembolism, treatment with an oral anticoagulant is appreciably more beneficial than treatment with an antiplatelet agent. Among 6,517 unselected patients with AF, 1,012 of them (15.5%) had a CHADS2 score of 1 and were liable to treatment with an antiplatelet agent or an anticoagulant. An oral anticoagulant was prescribed for 606 patients (59.9%) and an antiplatelet agent or no antithrombotic treatment for 406 (40.1%). During follow-up (median=793 days, interquartile range=1,332 days), 105 deaths (10.4%) and 19 strokes (1.9%) were recorded. The administration of an anticoagulant was associated with a lower rate of events (relative risk=0.42, 95% confidence interval 0.29-0.60, p<0.0001) than when no anticoagulant was prescribed. Results remained similar after adjustment for age and other confounding factors. In contrast, prescription of an antiplatelet agent was not associated with a lower risk of events. Factors independently associated with an increased risk of events were older age (p<0.0001), concomitant heart failure (p=0.0002), diabetes (p=0.0025), lack of prescription of an anticoagulant (p<0.0001) and permanent AF (p=0.04). Thus, prescription of an anticoagulant is independently associated with a decreased risk of death or stroke among patients with AF and a CHADS2 score =1.

  18. Source and transport of human enteric viruses in deep municipal water supply wells

    USGS Publications Warehouse

    Bradbury, Kenneth R.; Borchardt, Mark A.; Gotkowitz, Madeline; Spencer, Susan K.; Zhu, Jun; Hunt, Randall J.

    2013-01-01

    Until recently, few water utilities or researchers were aware of possible virus presence in deep aquifers and wells. During 2008 and 2009 we collected a time series of virus samples from six deep municipal water-supply wells. The wells range in depth from approximately 220 to 300 m and draw water from a sandstone aquifer. Three of these wells draw water from beneath a regional aquitard, and three draw water from both above and below the aquitard. We also sampled a local lake and untreated sewage as potential virus sources. Viruses were detected up to 61% of the time in each well sampled, and many groundwater samples were positive for virus infectivity. Lake samples contained viruses over 75% of the time. Virus concentrations and serotypes observed varied markedly with time in all samples. Sewage samples were all extremely high in virus concentration. Virus serotypes detected in sewage and groundwater were temporally correlated, suggesting very rapid virus transport, on the order of weeks, from the source(s) to wells. Adenovirus and enterovirus levels in the wells were associated with precipitation events. The most likely source of the viruses in the wells was leakage of untreated sewage from sanitary sewer pipes.

  19. Special Issue: Honey Bee Viruses

    PubMed Central

    Gisder, Sebastian; Genersch, Elke

    2015-01-01

    Pollination of flowering plants is an important ecosystem service provided by wild insect pollinators and managed honey bees. Hence, losses and declines of pollinating insect species threaten human food security and are of major concern not only for apiculture or agriculture but for human society in general. Honey bee colony losses and bumblebee declines have attracted intensive research interest over the last decade and although the problem is far from being solved we now know that viruses are among the key players of many of these bee losses and bumblebee declines. With this special issue on bee viruses we, therefore, aimed to collect high quality original papers reflecting the current state of bee virus research. To this end, we focused on newly discovered viruses (Lake Sinai viruses, bee macula-like virus), or a so far neglected virus species (Apis mellifera filamentous virus), and cutting edge technologies (mass spectrometry, RNAi approach) applied in the field. PMID:26702462

  20. Special Issue: Honey Bee Viruses.

    PubMed

    Gisder, Sebastian; Genersch, Elke

    2015-10-01

    Pollination of flowering plants is an important ecosystem service provided by wild insect pollinators and managed honey bees. Hence, losses and declines of pollinating insect species threaten human food security and are of major concern not only for apiculture or agriculture but for human society in general. Honey bee colony losses and bumblebee declines have attracted intensive research interest over the last decade and although the problem is far from being solved we now know that viruses are among the key players of many of these bee losses and bumblebee declines. With this special issue on bee viruses we, therefore, aimed to collect high quality original papers reflecting the current state of bee virus research. To this end, we focused on newly discovered viruses (Lake Sinai viruses, bee macula-like virus), or a so far neglected virus species (Apis mellifera filamentous virus), and cutting edge technologies (mass spectrometry, RNAi approach) applied in the field.

  1. Underuse of Anticoagulation in Older Patients with Atrial Fibrillation and CHADS2 Score ≥ 2: Are We Doing Better Since the Marketing of Direct Oral Anticoagulants?

    PubMed

    Henrard, Séverine; Vandenabeele, Caroline; Marien, Sophie; Boland, Benoit; Dalleur, Olivia

    2017-11-01

    Our objectives were to (1) describe the evolution of the underuse of anticoagulants in older people with atrial fibrillation (AF) and a CHADS 2 score ≥ 2 since direct oral anticoagulants (DOACs) were introduced to the market and (2) describe factors associated with this underuse. We conducted a retrospective cross-sectional study including geriatric patients admitted during the pre-DOAC (2008-2011) and post-DOAC (2013-2015) periods in an academic hospital in Belgium. Five inclusion criteria were met: age ≥ 75 years, diagnosis of AF, indication for anticoagulation (CHADS 2 score ≥ 2), risk of functional decline (Identification of Seniors At Risk [ISAR] score ≥ 2), and comprehensive geriatric assessment. The use of anticoagulants and antiplatelets at home before admission was recorded. Risks of stroke and bleeding were calculated using CHADS 2 and HEMORR 2 HAGES scores, respectively. Three different logistic regression models were performed to describe the evolution of and factors associated with the underuse of anticoagulants after DOAC marketing. Anticoagulant underuse, present in 209 of 614 (34%) geriatric patients with AF, was lower in patients with a history of stroke (28.5%) or congestive heart failure (26.9%) but higher in those receiving antiplatelets (56.2%) and in older individuals. Anticoagulant underuse decreased significantly from the pre-DOAC (37.3%) to the post-DOAC (29.7%) era, as shown by two analyses using propensity scores. In older patients with AF, anticoagulant underuse was mainly associated with antiplatelet use. Anticoagulant underuse and antiplatelet use have both decreased since DOAC marketing. Underuse of anticoagulants was still a concern for three in ten geriatric patients with AF at high risk of stroke (CHADS 2 score ≥ 2).

  2. Composition and cytotoxic activity of essential oils from Xylopia aethiopica (Dunal) A. Rich, Xylopia parviflora (A. Rich) Benth.) and Monodora myristica (Gaertn) growing in Chad and Cameroon

    PubMed Central

    2014-01-01

    Background Cancer has become a global public health problem and the search for new control measures is urgent. Investigation of plant products such as essential oils from Monodora myristica, Xylopia aethiopica and Xylopia parviflora might lead to new anticancer therapy. In this study, we have investigated the antineoplastic activity of essential oils from fruits of these plants growing in Chad and Cameroon. Methods The essential oils obtained by hydrodistillation of fruits of Monodora myristica, Xylopia aethiopica and Xylopia parviflora collected in Chad and Cameroon were analyzed by GC-FID and GC-MS and investigated for their antiproliferative activity against the breast cancer cell line (MCF7). Results Overall, monoterpenes were mostly found in the six essential oils. Oils from X. aethiopica and X. parviflora from Chad and Cameroon mainly contain β-pinene at 24.6%, 28.2%, 35.7% and 32.9% respectively. Monodora myristica oils from both origins contain mainly α-phellandrene at 52.7% and 67.1% respectively. The plant origin did not significantly influence the chemical composition of oils. The six essential oils exerted cytotoxic activity against cancer (MCF-7) and normal cell lines (ARPE-19), with more pronounced effect on neoplastic cells in the majority of cases. The highest selectivity was obtained with the essential oils of X. parviflora from Chad and Cameroon (5.87 and 5.54) which were more cytotoxic against MCF-7 than against normal cell line (ARPE-19) with IC50 values of 0.155 μL/mL and 0.166 μL/mL respectively. Conclusions Essential oils from fruits of Monodora myristica, Xylopia aethiopica and Xylopia parviflora have shown acceptable antineoplastic potency, and might be investigated further in this regard. PMID:24708588

  3. Effects of a behavior change campaign on household drinking water disinfection in the Lake Chad basin using the RANAS approach.

    PubMed

    Lilje, Jonathan; Mosler, Hans-Joachim

    2018-04-01

    Worldwide, an estimated 700 million people rely on unimproved drinking water sources; even more consume water that is not safe to drink. Inadequate drinking water quality constitutes a major risk factor for cholera and other diarrheal diseases around the globe, especially for young children in developing countries. Household water treatment and safe storage systems represent an intermediate solution for settings that lack infrastructure supplying safe drinking water. However, the correct and consistent usage of such treatment technologies rely almost exclusively on the consumer's behavior. This study targeted at evaluating effects of a behavior change campaign promoting the uptake of household drinking water chlorination in communities along the Chari and Logone rivers in Chad. The campaign was based on formative research using health psychological theory and targeted several behavioral factors identified as relevant. A total of 220 primary caregivers were interviewed concerning their household water treatment practices and mindset related to water treatment six months after the campaign. The Risks, Attitudes, Norms, Abilities, and Self-regulation (RANAS) model was used to structure the interviews as the RANAS approach had been used for designing the campaign. Results show significantly higher self-reported drinking water chlorination among participants of the intervention. Significant differences from a control group were identified regarding several behavioral factors. Mediation analysis revealed that the intervention positively affected participants' individual risk estimation for diarrheal disease, health knowledge, perceived efforts and benefits of water treatment, social support strategies, knowledge of how to perform chlorination, and perceived ability to do so. The campaign's effect on water treatment was mainly mediated through differences in health knowledge, changes in norms, and self-efficacy convictions. The findings imply that water treatment behavior

  4. 3 CFR - Drawdown Under Section 506(a)(1) of the Foreign Assistance Act of 1961, as Amended, for Chad and...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 3 The President 1 2014-01-01 2014-01-01 false Drawdown Under Section 506(a)(1) of the Foreign Assistance Act of 1961, as Amended, for Chad and France To Support Their Efforts in Mali Presidential Documents Other Presidential Documents Presidential Determination No. 2013-6 of February 11, 2013 Drawdown Under Section 506(a)(1) of the Foreign...

  5. From bench to almost bedside: the long road to a licensed Ebola virus vaccine.

    PubMed

    Wong, Gary; Mendoza, Emelissa J; Plummer, Francis A; Gao, George F; Kobinger, Gary P; Qiu, Xiangguo

    2018-02-01

    The Ebola virus (EBOV) disease epidemic during 2014-16 in West Africa has accelerated the clinical development of several vaccine candidates that have demonstrated efficacy in the gold standard nonhuman primate (NHP) model, namely cynomolgus macaques. Areas covered: This review discusses the pre-clinical research and if available, clinical evaluation of the currently available EBOV vaccine candidates, while emphasizing the translatability of pre-clinical data generated in the NHP model to clinical data in humans. Expert opinion: Despite the existence of many successful EBOV vaccine candidates in the pre-clinical stages, only two platforms became the focus of Phase 2/3 efficacy trials in Liberia, Sierra Leone, and Guinea near the peak of the epidemic: the Vesicular stomatitis virus (VSV)-vectored vaccine and the chimpanzee adenovirus type 3 (ChAd3)-vectored vaccine. The results of three distinct clinical trials involving these candidates may soon pave the way for a licensed, safe and efficacious EBOV vaccine to help combat future epidemics.

  6. From bench to almost bedside: The long road to a licensed Ebola virus vaccine

    PubMed Central

    Wong, Gary; Mendoza, Emelissa J.; Plummer, Francis A.; Gao, George F.; Kobinger, Gary P.; Qiu, Xiangguo

    2018-01-01

    Introduction The Ebola virus (EBOV) disease epidemic during 2014-16 in West Africa has accelerated the clinical development of several vaccine candidates that have demonstrated efficacy in the gold standard nonhuman primate (NHP) model, namely cynomolgus macaques. Areas covered This review discusses the pre-clinical research and if available, clinical evaluation of the currently available EBOV vaccine candidates, while emphasizing the translatability of pre-clinical data generated in the NHP model to clinical data in humans. Expert opinion Despite the existence of many successful EBOV vaccine candidates in the pre-clinical stages, only two platforms became the focus of Phase 2/3 efficacy trials in Liberia, Sierra Leone, and Guinea near the peak of the epidemic: the Vesicular stomatitis virus (VSV)-vectored vaccine and the chimpanzee adenovirus type 3 (ChAd3)-vectored vaccine. The results of three distinct clinical trials involving these candidates may soon pave the way for a licensed, safe and efficacious EBOV vaccine to help combat future epidemics. PMID:29148858

  7. Human and Animal Vaccination Delivery to Remote Nomadic Families, Chad

    PubMed Central

    Bechir, Mahamat; Ahmed, Mahamat Abdoulaye; Wyss, Kaspar; Randolph, Thomas F.; Zinsstag, Jakob

    2007-01-01

    Vaccination services for people and livestock often fail to achieve sufficient coverages in Africa’s remote rural settings because of financial, logistic, and service delivery constraints. In Chad from 2000 through 2005, we demonstrated the feasibility of combining vaccination programs for nomadic pastoralists and their livestock. Sharing of transport logistics and equipment between physicians and veterinarians reduced total costs. Joint delivery of human and animal health services is adapted to and highly valued by hard-to-reach pastoralists. In intervention zones, for the first time ≈10% of nomadic children (>1–11 months of age) were fully immunized annually and more children and women were vaccinated per day during joint vaccination rounds than during vaccination of persons only and not their livestock (130 vs. 100, p<0.001). By optimizing use of limited logistical and human resources, public health and veterinary services both become more effective, especially at the district level. PMID:17552089

  8. Isolation of viral haemorrhagic septicaemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St Clair, Michigan, USA reveals a new sublineage of the North American genotype

    USGS Publications Warehouse

    Elsayed, E.; Faisal, M.; Thomas, M.; Whelan, G.; Batts, W.; Winton, J.

    2006-01-01

    Viral haemorrhagic septicaemia virus (VHSV) was isolated from muskellunge, Esox masquinongy (Mitchill), caught from the NW portion of Lake St Clair, Michigan, USA in 2003. Affected fish exhibited congestion of internal organs; the inner wall of the swim bladder was thickened and contained numerous budding, fluid-filled vesicles. A virus was isolated using fish cell lines inoculated with a homogenate of kidney and spleen tissues from affected fish. Focal areas of cell rounding and granulation appeared as early as 24 h post-inoculation and expanded rapidly to destroy the entire cell sheet by 96 h. Electron microscopy revealed virions that were 170-180 nm in length by 60-70 nm in width having a bullet-shaped morphology typical of rhabdoviruses. The virus was confirmed as VHSV by reverse transcriptase-polymerase chain reaction. Sequence analysis of the entire nucleoprotein and glycoprotein genes revealed the virus was a member of the North American genotype of VHSV; however, the isolate was sufficiently distinct to be considered a separate sublineage, suggesting its origin may have been from marine species inhabiting the eastern coastal areas of the USA or Canada.

  9. Water-quality and biological data for selected streams, lakes, and wells in the High Point Lake watershed, Guilford County, North Carolina, 1988-89

    USGS Publications Warehouse

    Davenport, M.S.

    1993-01-01

    Water and bottom-sediment samples were collected at 26 sites in the 65-square-mile High Point Lake watershed area of Guilford County, North Carolina, from December 1988 through December 1989. Sampling locations included 10 stream sites, 8 lake sites, and 8 ground-water sites. Generally, six steady-flow samples were collected at each stream site and three storm samples were collected at five sites. Four lake samples and eight ground-water samples also were collected. Chemical analyses of stream and lake sediments and particle-size analyses of lake sediments were performed once during the study. Most stream and lake samples were analyzed for field characteristics, nutrients, major ions, trace elements, total organic carbon, and chemical-oxygen demand. Analyses were performed to detect concentrations of 149 selected organic compounds, including acid and base/neutral extractable and volatile constituents and carbamate, chlorophenoxy acid, triazine, organochlorine, and organophosphorus pesticides and herbicides. Selected lake samples were analyzed for all constituents listed in the Safe Drinking Water Act of 1986, including Giardia, Legionella, radiochemicals, asbestos, and viruses. Various chromatograms from organic analyses were submitted to computerized library searches. The results of these and all other analyses presented in this report are in tabular form.

  10. Avian Influenza Virus Surveillance in High Arctic Breeding Geese, Greenland.

    PubMed

    Gaidet, Nicolas; Leclercq, India; Batéjat, Christophe; Grassin, Quentin; Daufresne, Tanguy; Manuguerra, Jean-Claude

    2018-06-01

    The connectedness in Arctic regions between migratory waterbird populations originating from different continents and the potential for virus exchange at their shared Arctic breeding ground point to the need to explore the largely unstudied circumpolar circulation of avian influenza viruses (AIV). We here report the investigation of AIV in wild birds and lakes in a high Arctic area of Northeast Greenland. No AIV could be detected in the fecal, feather, and water samples collected from large flocks of pink-footed geese Anser brachyrhynchus and barnacle geese Branta leucopsis in and around refuge lakes, where they congregate at high density during their flightless molting period in summer.

  11. Isolation and characterization of a virus infecting the freshwater algae Chrysochromulina parva

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mirza, S.F.; Staniewski, M.A.; Short, C.M.

    Water samples from Lake Ontario, Canada were tested for lytic activity against the freshwater haptophyte algae Chrysochromulina parva. A filterable lytic agent was isolated and identified as a virus via transmission electron microscopy and molecular methods. The virus, CpV-BQ1, is icosahedral, ca. 145 nm in diameter, assembled within the cytoplasm, and has a genome size of ca. 485 kb. Sequences obtained through PCR-amplification of DNA polymerase (polB) genes clustered among sequences from the family Phycodnaviridae, whereas major capsid protein (MCP) sequences clustered among sequences from either the Phycodnaviridae or Mimiviridae. Based on quantitative molecular assays, C. parva's abundance in Lakemore » Ontario was relatively stable, yet CpV-BQ1's abundance was variable suggesting complex virus-host dynamics. This study demonstrates that CpV-BQ1 is a member of the proposed order Megavirales with characteristics of both phycodnaviruses and mimiviruses indicating that, in addition to its complex ecological dynamics, it also has a complex evolutionary history. - Highlights: • A virus infecting the algae C. parva was isolated from Lake Ontario. • Virus characteristics demonstrated that this novel virus is an NCLDV. • The virus's polB sequence suggests taxonomic affiliation with the Phycodnaviridae. • The virus's capsid protein sequences also suggest Mimiviridae ancestry. • Surveys of host and virus natural abundances revealed complex host–virus dynamics.« less

  12. Lake Granbury and Lake Whitney Assessment Initiative Final Scientific/Technical Report Summary

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Harris, B. L.; Roelke, Daniel; Brooks, Bryan

    blooms. Our numerical modeling results support the idea that cyanobacteria, through allelopathy, control the timing of golden algae blooms in Lake Granbury. The in-lake experiments in Lake Whitney and Lake Waco also revealed that as golden algae blooms develop, there are natural enemies (a species of rotifer, and a virus) that help slow the population growth. Again, better characterization of these organisms is a high priority as it may be key to managing golden algae blooms. Our laboratory and in-lake experiments and field monitoring have shown that nutrient additions will remove toxicity and prevent golden algae from blooming. In fact, other algae displace the golden algae after nutrient additions. Additions of ammonia are particularly effective, even at low doses (much lower than what is employed in fish hatchery ponds). Application of ammonia in limited areas of lakes, such as in coves, should be explored as a management option. The laboratory experiments and field monitoring also show that the potency of toxins produced by P. parvum is greatly reduced when water pH is lower, closer to neutral levels. Application of mild acid to limited areas of lakes (but not to a level where acidic conditions are created), such as in coves, should be explored as a management option. Finally, our field monitoring and mathematical modeling revealed that flushing/dilution at high enough levels could prevent P. parvum from forming blooms and/or terminate existing blooms. This technique could work using deeper waters within a lake to flush the surface waters of limited areas of the same lakes, such as in coves and should be explored as a management option. In this way, water releases from upstream reservoirs would not be necessary and there would be no addition of nutrients in the lake.« less

  13. Vector competence of the stable fly (Diptera: Muscidae)for West Nile virus.

    USDA-ARS?s Scientific Manuscript database

    Stable flies, which are notorious pests of cattle and other livestock, were suspected of transmitting West Nile virus (WNV) among American white pelicans at the Medicine Lake Wildlife Refuge in northeastern Montana in 2006-2007. However the ability of stable flies to transmit the virus was unknown. ...

  14. Using space photography of the Earth in the classroom: Drought in Africa

    NASA Technical Reports Server (NTRS)

    Kinsler, Margaret C.

    1986-01-01

    A script of a classroom presentation based on space photographs of Africa is presented. The intended audience is elementary and secondary school teachers. Photographs of Lake Chad are used to illustrate climatic developments that exemplify the African drought problem.

  15. Two reassortant types of highly pathogenic H5N8 avian influenza virus from wild birds in Central China in 2016.

    PubMed

    Ma, Liping; Jin, Tao; Wang, Hanzhong; Liu, Haizhou; Wang, Runkun; Li, Yong; Yang, Guoxiang; Xiong, Yanping; Chen, Jing; Zhang, Jun; Chen, Guang; Li, Wei; Liu, Di; Lin, Peng; Huang, Yueying; Gao, George F; Chen, Quanjiao

    2018-02-07

    Since 2016, the highly pathogenic avian influenza H5N8 virus has emerged in the Central Asian flyway and Europe, causing massive deaths in poultry and wild birds. In this study, we isolated and identified three H5N8 viruses from swan goose and black swans in Hubei province during the 2016/2017 winter season. Whole-genome sequencing and phylogenetic analysis revealed that the three viruses clustered into a group of H5N8 viruses from Qinghai Lake and Europe. A novel reassortment virus from swan goose was distinguished from that of black swans, in that its PA and NP genes were distinct from those of Qinghai Lake viruses. Molecular dating revealed that the ancestral strain of these H5N8 viruses emerged around July 2015. From sequence comparison, we discovered eight amino acid substitutions in HA and NA during the adaption process from poultry to wild birds. The three viruses were isolated from wild birds in the East Asian-Australasian flyway; however, the viral genomes were similar to H5N8 viruses circulating along the Central Asian flyway. From these data, we conclude that wetlands and lakes in Central China may play a key role in disseminating H5N8 viruses between the East Asian-Australasian and Central Asian flyways.

  16. Meta-analysis of CHADS2 versus CHA2DS2-VASc for predicting stroke and thromboembolism in atrial fibrillation patients independent of anticoagulation.

    PubMed

    Zhu, Wen-Gen; Xiong, Qin-Mei; Hong, Kui

    2015-02-01

    Two validated scoring systems for predicting embolic risk, CHADS2 and CHA2DS2-VASc, contribute to optimizing antithrombotic prescription practices in patients who have atrial fibrillation. However, data about anticoagulated patients are sparse. We compared CHADS2 and CHA2DS2-VASc, in terms of their predictive risk evaluation, in patients with atrial fibrillation who were and were not taking anticoagulants. We systematically searched the Cochrane Library, PubMed, and Embase databases for studies of the comparative diagnostic performance of CHADS2 and CHA2DS2-VASc. We identified 12 cohort studies for meta-analysis. With regard to the occurrence of cardiovascular events individually, patients with CHA2DS2-VASc scores ≥2 have a greater risk of stroke (risk ratio [RR]=5.15; 95% confidence interval [CI], 3.85-6.88; P <0.00001) and thromboembolism (RR=5.96; 95% CI, 5.50-6.45; P <0.00001) (P diff=0.34) than do patients with CHA2DS2-VASc scores <2, independent of anticoagulation therapy (RR=5.76; 95% CI, 5.23-6.35; P <0.00001 in anticoagulated patients; and RR=6.12; 95% CI, 5.40-6.93; P <0.00001 in patients not taking anticoagulants; P diff=0.45). The pooled RR estimates indicate an approximate 6-fold increase in the risk of endpoint events in patients with CHA2DS2-VASc scores ≥2 (RR=5.90; 95% CI, 5.46-6.37; P <0.0001). These results clearly indicate the discriminative capacity of the CHA2DS2-VASc score for stroke, thromboembolic events, or both, independent of optimal anticoagulation. The CHA2DS2-VASc score enables the identification of patients who are at genuinely high risk and can direct the selection of appropriate therapeutic approaches.

  17. Economic benefits of keeping vaccines at ambient temperature during mass vaccination: the case of meningitis A vaccine in Chad

    PubMed Central

    Zipursky, Simona; Tevi-Benissan, Carole; Djingarey, Mamoudou Harouna; Gbedonou, Placide; Youssouf, Brahim Oumar; Zaffran, Michel

    2014-01-01

    Abstract Objective To evaluate the potential economic benefits of keeping a meningitis A vaccine at or near ambient temperature for up to 4 days during a mass vaccination campaign. Methods During a 10-day mass vaccination campaign against meningitis A in three regions of Chad in 2011, the costs associated with storage and transport of the vaccine in a traditional cold chain system were evaluated. A mathematical model was used to estimate the savings that could have been achieved if the vaccine had been stored at or near ambient temperature – in a “controlled temperature” chain – at the peripheral levels of the supply chain system. Findings The cost of the cold chain and associated logistics used in the campaign in Chad was 0.24 United States dollars (US$) per person vaccinated. In the modelled scenario for a controlled temperature chain, however, these costs dropped by 50% and were estimated to be only US$ 0.12 per person vaccinated. Conclusion The implementation of a “controlled temperature” chain at the most peripheral levels of the supply chain system – assuming no associated loss of vaccine potency, efficacy or safety – could result in major economic benefits and allow vaccine coverage to be extended in low-resource settings. PMID:24623901

  18. Low prevalence of VHSV detected in round goby collected in offshore regions of Lake Ontario

    USGS Publications Warehouse

    Cornwell, Emily R.; Getchell, Rodman G.; Groocock, Geoffrey H.; Walsh, Maureen G.; Bowser, Paul R.

    2012-01-01

    Since the first reports of mortalities due to viral hemorrhagic septicemia virus (VHSV) type IVb in the Laurentian Great Lakes basin during 2005 (Lake St. Clair, USA and Bay of Quinte, Lake Ontario, Canada), many groups have conducted surveillance efforts for the virus, primarily in nearshore areas. The round goby (Neogobius melanostomus) has been identified as a key species to target for surveillance, because they have a very high probability of infection at a given site. Our objective in this study was to document and quantify VHSV in round gobies in offshore waters of Lake Ontario using molecular techniques. We collected 139 round gobies from depths ranging from 55 to 150 m using bottom trawls during the early spring of 2011 and detected VHSV in 4 individuals (1/26 fish at 95 m, 2/12 fish at 105 m, and 1/24 fish at 135 m). These results expand the known depth range of VHSV in the Great Lakes. They also have implications on the management of the spread of VHSV within infected bodies of water related to the mixing of populations of fish that would remain distinct in their breeding habitats, but then have the opportunity to mix in their overwintering habitats, as well as to increase overlap of predator and prey species in overwintering habitats.

  19. Regional framework, structural and petroleum aspects of rift basins in Niger, Chad and the Central African Republic (C.A.R.)

    NASA Astrophysics Data System (ADS)

    Genik, G. J.

    1992-10-01

    This paper overviews the regional framework, tectonic, structural and petroleum aspects of rifts in Niger, Chad and the C.A.R. The data base is from mainly proprietary exploration work consisting of some 50,000 kilometres of seismic profiles, 50 exploration wells, one million square kilometres of aeromagnetics coverage and extensive gravity surveys. There have been 13 oil and two oil and gas discoveries. A five phased tectonic history dating from the Pan African orogeny (750-550 MY B.P.) to the present suggests that the Western Central African Rift System (WCAS) with its component West African Rift Subsystem (WAS) and Central African Subsystem (CAS) formed mainly by the mechanical separation of African crustal blocks during the Early Cretaceous. Among the resulting rift basins in Niger, Chad and the C.A.R., seven are in the WAS—Grein, Kafra, Tenere. Tefidet, Termit, Bongor, and N'Dgel Edgi and three, Doba, Doseo, and Salamat are in the CAS. The WAS basins in Niger and Chad are all extensional and contain more than 14,000 m of continental to marine Early Cretaceous to Recent clastic sediments and minor amounts of volcanics. Medium to light oil (20° API-46° API) and gas have been discovered in the Termit basin in reservoir, source and seal beds of Late Cretaceous and Palaeogene age. The most common structural styles are extensional normal fault blocks and transtensional synthetic and antithetic normal fault blocks. The CAS Doba, Doseo and Salamat are extensional to transtensional rift basins containing up to 7500 m of terrestrial mainly Early Cretaceous clastics. Heavy to light oil (15°-39° API) and gas have been discovered in Doba and Doseo basins. Source rocks are Early Cretaceous lacustrine shales, whereas reservoirs and seals are both Early and Late Cretaceous. Dominant structural styles are extensional and transtensional fault blocks, transpressional anticlines and flower structures. The existence of a total rift basin sediment volume of more than one

  20. Technical Education and Vocational Training in Central Africa. Feasibility Survey of the Regional Development of Rapid Vocational Training: Cameroon, Central African Republic, Chad, and Gabon.

    ERIC Educational Resources Information Center

    Organization for Rehabilitation through Training, Geneva (Switzerland).

    This final report is the result of a survey requested by the United States Agency for International Development (USAID) and undertaken by the Organization for Rehabilitation through Training (ORT) of four countries (Cameroon, Chad, Central African Republic, and Gabon) and a conference on vocational training sponsored by the Economic and Customs…

  1. Cle Elum Lake Sockeye Salmon Restoration Feasibility Study, 1986-1988 Progress Report.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Flagg, Thomas A.

    1988-10-01

    In 1986, a multi-year project to evaluate the biological feasibility of reestablishing anadromous sockeye salmon (Oncorhynchus nerka) runs to Cle Elum Lake in the Yakima River Basin was established between the Bonneville Power Administration (BPA) and the National Marine Fisheries Service (NMFS). This program involves the capture, spawning, and rearing of disease-free donor stock in 1987 and 1988 and assessment of juvenile outmigration and survival from Cle Elum Lake in 1989 and 1990. Work in 1987--1988 involved collection of adult sockeye salmon from the Lake Wenatchee run and incubation and rearing of progeny as donor stock. In July 1987, 263more » adults were captured at the Dryden fishway on the Wenatchee River and transferred to net-pens in Lake Wenatchee. Adults were held approximately 90 days and spawned, and the eggs were transferred to a quarantine hatchery. Pre-spawning survival was 95.1%, and all spawners were certified as being free of Infectious Hematopoietic Necrosis (IHN) and other replicating viruses. Egg viability averaged about 40%; however, eyed egg to hatch survival was over 99%. Juveniles are being reared in quarantine, and survival to date is about 92%. The NMFS currently has over 131,000 fry (0.7 g average weight) in culture. Fry have been certified twice (at 0.12 g and 0.25 g average weight) as being free of IHN and other replicating viruses. Viral certification will continue throughout rearing. 13 refs., 4 figs., 3 tabs.« less

  2. Dust as a tipping element: The Bodélé Depression, Chad

    PubMed Central

    Washington, Richard; Bouet, Christel; Cautenet, Guy; Mackenzie, Elisabeth; Ashpole, Ian; Engelstaedter, Sebastian; Lizcano, Gil; Henderson, Gideon M.; Schepanski, Kerstin; Tegen, Ina

    2009-01-01

    Dust plays a vital role in climate and biophysical feedbacks in the Earth system. One source of dust, the Bodélé Depression in Chad, is estimated to produce about half the mineral aerosols emitted from the Sahara, which is the world's largest source. By using a variety of new remote sensing data, regional modeling, trajectory models, chemical analyses of dust, and future climate simulations, we investigate the current and past sensitivity of the Bodélé. We show that minor adjustments to small features of the atmospheric circulation, such as the Bodélé Low-Level Jet, could profoundly alter the behavior of this feature. Dust production during the mid-Holocene ceased completely from this key source region. Although subject to a great deal of uncertainty, some simulations of the 21st century indicate the potential for a substantial increase in dust production by the end of the century in comparison with current values. PMID:19620716

  3. Detection of viral hemorrhagic septicemia virus

    USGS Publications Warehouse

    Winton, James; Kurath, Gael; Batts, William

    2007-01-01

    Viral hemorrhagic septicemia virus (VHSV) is considered to be one of the most important viral pathogens of finfish and is listed as reportable by many nations and international organizations (Office International des Epizooties 2006). Prior to 1988, VHSV was thought to be limited to Europe (Wolf 1988; Smail 1999). Subsequently, it was shown that the virus is endemic among many marine and anadromous fish species in both the Pacific and Atlantic Oceans (Meyers and Winton 1995; Skall et al. 2005). Genetic analysis reveals that isolates of VHSV can be divided into four genotypes that generally correlate with geographic location with the North American isolates generally falling into VHSV Genotype IV (Snow et al. 2004). In 2005-2006, reports from the Great Lakes region indicated that wild fish had experienced disease or, in some cases, very large die-offs from VHSV (Elsayed et al. 2006, Lumsden et al. 2007). The new strain from the Great Lakes, now identified as VHSV Genotype IVb, appears most closely related to isolates of VHSV from mortalities that occurred during 2000-2004 in rivers and near-shore areas of New Brunswick and Nova Scotia, Canada (Gagne et al. 2007). The type IVb isolate found in the Great Lakes region is the only strain outside of Europe that has been associated with significant mortality in freshwater species.

  4. Metagenomics Reveals a Novel Virophage Population in a Tibetan Mountain Lake

    PubMed Central

    Oh, Seungdae; Yoo, Dongwan; Liu, Wen-Tso

    2016-01-01

    Virophages are parasites of giant viruses that infect eukaryotic organisms and may affect the ecology of inland water ecosystems. Despite the potential ecological impact, limited information is available on the distribution, diversity, and hosts of virophages in ecosystems. Metagenomics revealed that virophages were widely distributed in inland waters with various environmental characteristics including salinity and nutrient availability. A novel virophage population was overrepresented in a planktonic microbial community of the Tibetan mountain lake, Lake Qinghai. Our study identified coccolithophores and coccolithovirus-like phycodnaviruses in the same community, which may serve as eukaryotic and viral hosts of the virophage population, respectively. PMID:27151658

  5. Bacillus anthracis Diversity and Geographic Potential across Nigeria, Cameroon and Chad: Further Support of a Novel West African Lineage

    PubMed Central

    Blackburn, Jason K.; Odugbo, Moses Ode; Van Ert, Matthew; O’Shea, Bob; Mullins, Jocelyn; Perrenten, Vincent; Maho, Angaya; Hugh-Jones, Martin; Hadfield, Ted

    2015-01-01

    Zoonoses, diseases affecting both humans and animals, can exert tremendous pressures on human and veterinary health systems, particularly in resource limited countries. Anthrax is one such zoonosis of concern and is a disease requiring greater public health attention in Nigeria. Here we describe the genetic diversity of Bacillus anthracis in Nigeria and compare it to Chad, Cameroon and a broader global dataset based on the multiple locus variable number tandem repeat (MLVA-25) genetic typing system. Nigerian B. anthracis isolates had identical MLVA genotypes and could only be resolved by measuring highly mutable single nucleotide repeats (SNRs). The Nigerian MLVA genotype was identical or highly genetically similar to those in the neighboring countries, confirming the strains belong to this unique West African lineage. Interestingly, sequence data from a Nigerian isolate shares the anthrose deficient genotypes previously described for strains in this region, which may be associated with vaccine evasion. Strains in this study were isolated over six decades, indicating a high level of temporal strain stability regionally. Ecological niche models were used to predict the geographic distribution of the pathogen for all three countries. We describe a west-east habitat corridor through northern Nigeria extending into Chad and Cameroon. Ecological niche models and genetic results show B. anthracis to be ecologically established in Nigeria. These findings expand our understanding of the global B. anthracis population structure and can guide regional anthrax surveillance and control planning. PMID:26291625

  6. Viruses of haloarchaea.

    PubMed

    Luk, Alison W S; Williams, Timothy J; Erdmann, Susanne; Papke, R Thane; Cavicchioli, Ricardo

    2014-11-13

    In hypersaline environments, haloarchaea (halophilic members of the Archaea) are the dominant organisms, and the viruses that infect them, haloarchaeoviruses are at least ten times more abundant. Since their discovery in 1974, described haloarchaeoviruses include head-tailed, pleomorphic, spherical and spindle-shaped morphologies, representing Myoviridae, Siphoviridae, Podoviridae, Pleolipoviridae, Sphaerolipoviridae and Fuselloviridae families. This review overviews current knowledge of haloarchaeoviruses, providing information about classification, morphotypes, macromolecules, life cycles, genetic manipulation and gene regulation, and host-virus responses. In so doing, the review incorporates knowledge from laboratory studies of isolated viruses, field-based studies of environmental samples, and both genomic and metagenomic analyses of haloarchaeoviruses. What emerges is that some haloarchaeoviruses possess unique morphological and life cycle properties, while others share features with other viruses (e.g., bacteriophages). Their interactions with hosts influence community structure and evolution of populations that exist in hypersaline environments as diverse as seawater evaporation ponds, to hot desert or Antarctic lakes. The discoveries of their wide-ranging and important roles in the ecology and evolution of hypersaline communities serves as a strong motivator for future investigations of both laboratory-model and environmental systems.

  7. Spread of the Emerging Viral Hemorrhagic Septicemia Virus Strain, Genotype IVb, in Michigan, USA

    PubMed Central

    Faisal, Mohamed; Shavalier, Megan; Kim, Robert K.; Millard, Elena V.; Gunn, Michelle R.; Winters, Andrew D.; Schulz, Carolyn A.; Eissa, Alaa; Thomas, Michael V.; Wolgamood, Martha; Whelan, Gary E.; Winton, James

    2012-01-01

    In 2003, viral hemorrhagic septicemia virus (VHSV) emerged in the Laurentian Great Lakes causing serious losses in a number of ecologically and recreationally important fish species. Within six years, despite concerted managerial preventive measures, the virus spread into the five Great Lakes and to a number of inland waterbodies. In response to this emerging threat, cooperative efforts between the Michigan Department of Natural Resources (MI DNR), the Michigan State University Aquatic Animal Health Laboratory (MSU-AAHL), and the United States Department of Agriculture-Animal and Plant Health Inspection Services (USDA-APHIS) were focused on performing a series of general and VHSV-targeted surveillances to determine the extent of virus trafficking in the State of Michigan. Herein we describe six years (2005–2010) of testing, covering hundreds of sites throughout Michigan’s Upper and Lower Peninsulas. A total of 96,228 fish representing 73 species were checked for lesions suggestive of VHSV and their internal organs tested for the presence of VHSV using susceptible cell lines. Of the 1,823 cases tested, 30 cases from 19 fish species tested positive for VHSV by tissue culture and were confirmed by reverse transcriptase polymerase chain reaction (RT-PCR). Gene sequence analyses of all VHSV isolates retrieved in Michigan demonstrated that they belong to the emerging sublineage “b” of the North American VHSV genotype IV. These findings underscore the complexity of VHSV ecology in the Great Lakes basin and the critical need for rigorous legislation and regulatory guidelines in order to reduce the virus spread within and outside of the Laurentian Great Lakes watershed. PMID:22754647

  8. Evidence of Lake Trout reproduction at Lake Michigan's mid-lake reef complex

    USGS Publications Warehouse

    Janssen, J.; Jude, D.J.; Edsall, T.A.; Paddock, R.W.; Wattrus, N.; Toneys, M.; McKee, P.

    2006-01-01

    The Mid-Lake Reef Complex (MLRC), a large area of deep (> 40 m) reefs, was a major site where indigenous lake trout (Salvelinus namaycush) in Lake Michigan aggregated during spawning. As part of an effort to restore Lake Michigan's lake trout, which were extirpated in the 1950s, yearling lake trout have been released over the MLRC since the mid-1980s and fall gill net censuses began to show large numbers of lake trout in spawning condition beginning about 1999. We report the first evidence of viable egg deposition and successful lake trout fry production at these deep reefs. Because the area's existing bathymetry and habitat were too poorly known for a priori selection of sampling sites, we used hydroacoustics to locate concentrations of large fish in the fall; fish were congregating around slopes and ridges. Subsequent observations via unmanned submersible confirmed the large fish to be lake trout. Our technological objectives were driven by biological objectives of locating where lake trout spawn, where lake trout fry were produced, and what fishes ate lake trout eggs and fry. The unmanned submersibles were equipped with a suction sampler and electroshocker to sample eggs deposited on the reef, draw out and occasionally catch emergent fry, and collect egg predators (slimy sculpin Cottus cognatus). We observed slimy sculpin to eat unusually high numbers of lake trout eggs. Our qualitative approaches are a first step toward quantitative assessments of the importance of lake trout spawning on the MLRC.

  9. 77 FR 41686 - Safety Zone; Sheffield Lake Fireworks, Lake Erie, Sheffield Lake, OH

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-16

    ...-AA00 Safety Zone; Sheffield Lake Fireworks, Lake Erie, Sheffield Lake, OH AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on Lake Erie, Sheffield Lake, OH. This safety zone is intended to restrict vessels from a portion of Lake Erie...

  10. Earth observations during Space Shuttle flight STS-41 - Discovery's mission to planet earth

    NASA Technical Reports Server (NTRS)

    Lulla, Kamlesh P.; Helfert, Michael R.; Amsbury, David L.; Whitehead, Victor S.; Richards, Richard N.; Cabana, Robert D.; Shepherd, William M.; Akers, Thomas D.; Melnick, Bruce E.

    1991-01-01

    An overview of space flight STS-41 is presented, including personal observations and comments by the mission astronauts. The crew deployed the Ulysses spacecraft to study the polar regions of the sun and the interplanetary space above the poles. Environmental observations, including those of Lake Turkana, Lake Chad, biomass burning in Madagascar and Argentina, and circular features in Yucatan are described. Observations that include landforms and geology, continental sedimentation, desert landscapes, and river morphology are discussed.

  11. A reverse genetics system for the Great Lakes strain of viral hemorrhagic septicemia virus: the NV gene is required for pathogenicity

    USGS Publications Warehouse

    Ammayappan, Arun; Kurath, Gael; Thompson, Tarin M.; Vakharia, Vikram N.

    2011-01-01

    Viral hemorrhagic septicemia virus (VHSV), belonging to the genus Novirhabdovirus in the family of Rhabdoviridae, causes a highly contagious disease of fresh and saltwater fish worldwide. Recently, a novel genotype of VHSV, designated IVb, has invaded the Great Lakes in North America, causing large-scale epidemics in wild fish. An efficient reverse genetics system was developed to generate a recombinant VHSV of genotype IVb from cloned cDNA. The recombinant VHSV (rVHSV) was comparable to the parental wild-type strain both in vitro and in vivo, causing high mortality in yellow perch (Perca flavescens). A modified recombinant VHSV was generated in which the NV gene was substituted with an enhanced green fluorescent protein gene (rVHSV-ΔNV-EGFP), and another recombinant was made by inserting the EGFP gene into the full-length viral clone between the P and M genes (rVHSV-EGFP). The in vitro replication kinetics of rVHSV-EGFP was similar to rVHSV; however, the rVHSV-ΔNV-EGFP grew 2 logs lower. In yellow perch challenges, wtVHSV and rVHSV induced 82-100% cumulative per cent mortality (CPM), respectively, whereas rVHSV-EGFP produced 62% CPM and rVHSV-ΔNV-EGFP caused only 15% CPM. No reversion of mutation was detected in the recovered viruses and the recombinant viruses stably maintained the foreign gene after several passages. These results indicate that the NV gene of VHSV is not essential for viral replication in vitro and in vivo, but it plays an important role in viral replication efficiency and pathogenicity. This system will facilitate studies of VHSV replication, virulence, and production of viral vectored vaccines.

  12. Lake trout rehabilitation in Lake Ontario

    USGS Publications Warehouse

    Elrod, Joseph H.; O'Gorman, Robert; Schneider, Clifford P.; Eckert, Thomas H.; Schaner, Ted; Bowlby, James N.; Schleen, Larry P.

    1995-01-01

    Attempts to maintain the native lake trout (Salvelinus namaycush) population in Lake Ontario by stocking fry failed and the species was extirpated by the 1950s. Hatchery fish stocked in the 1960s did not live to maturity because of sea lamprey (Petromyzon marinus) predation and incidental commercial harvest. Suppression of sea lampreys began with larvicide treatments of Lake Ontario tributaries in 1971 and was enhanced when the tributaries of Oneida Lake and Lake Erie were treated in the 1980s. Annual stocking of hatchery fish was resumed with the 1972 year class and peaked at about 1.8 million yearlings and 0.3 million fingerlings from the 1985–1990 year classes. Survival of stocked yearlings declined over 50% in the 1980 s and was negatively correlated with the abundance of lake trout > 550 mm long (r = −0.91, P < 0.01, n = 12). A slot length limit imposed by the State of New York for the 1988 fishing season reduced angler harvest. Angler harvest in Canadian waters was 3 times higher in eastern Lake Ontario than in western Lake Ontario. For the 1977–1984 year classes, mean annual survival rate of lake trout age 6 and older was 0.45 (range: 0.35–0.56). In U.S. waters during 1985–1992, the total number of lake trout harvested by anglers was about 2.4 times greater than that killed by sea lampreys. The number of unmarked lake trout < 250 mm long in trawl catches in 1978–1992 was not different from that expected due to loss of marks and failure to apply marks at the hatchery, and suggested that recruitment of naturally-produced fish was nil. However, many of the obstacles which may have impeded lake trout rehabilitation in Lake Ontario during the 1980s are slowly being removed, and there are signs of a general ecosystem recovery. Significant recruitment of naturally produced lake trout by the year 2000, one interim objective of the rehabilitation plan for the Lake, may be achieved.

  13. Lake level variability in Silver Lake, Michigan: a response to fluctuations in lake levels of Lake Michigan

    USGS Publications Warehouse

    Fisher, Timothy G.; Loope, Walter L.

    2004-01-01

    Sediment from Silver Lake, Michigan, can be used to constrain the timing and elevation of Lake Michigan during the Nipissing transgression. Silver Lake is separated from Lake Michigan by a barrier/dune complex and the Nipissing, Calumet, and Glenwood shorelines of Lake Michigan are expressed landward of this barrier. Two Vibracores were taken from the lake in February 2000 and contain pebbly sand, sand, buried soils, marl, peat, and sandy muck. It is suggested here that fluctuations in the level of Lake Michigan are reflected in Silver Lake since the Chippewa low phase, and possibly at the end of the Algonquin phase. An age of 12,490 B.P. (10,460±50 14C yrs B.P.) on wood from a buried Entisol may record the falling Algonquin phase as the North Bay outlet opened. A local perched water table is indicated by marl deposited before 7,800 B.P. and peat between 7,760-7,000 B.P. when Lake Michigan was at the low elevation Chippewa phase. Continued deepening of the lake is recorded by the transition from peat to sandy muck at 7,000 B.P. in the deeper core, and with the drowning of an Inceptisol nearly 3 m higher at 6,410 B.P. in the shallower core. A rising groundwater table responding to a rising Lake Michigan base level during the Nipissing transgression, rather than a response to mid-Holocene climate change, explains deepening of Silver Lake. Sandy muck was deposited continually in Silver Lake between Nipissing and modern time. Sand lenses within the muck are presumed to be eolian in origin, derived from sand dunes advancing into the lake on the western side of the basin.

  14. Hydrologic, land cover, and seasonal patterns of waterborne pathogens in Great Lakes tributaries

    USGS Publications Warehouse

    Lenaker, Peter L.; Corsi, Steven; Borchardt, Mark A.; Spencer, Susan K.; Baldwin, Austin K.; Lutz, Michelle A.

    2017-01-01

    Great Lakes tributaries are known to deliver waterborne pathogens from a host of sources. To examine the hydrologic, land cover, and seasonal patterns of waterborne pathogens (i.e. protozoa (2), pathogenic bacteria (4) human viruses, (8) and bovine viruses (8)) eight rivers were monitored in the Great Lakes Basin over 29 months from February 2011 to June 2013. Sampling locations represented a wide variety of land cover classes from urban to agriculture to forest. A custom automated pathogen sampler was deployed at eight sampling locations which provided unattended, flow-weighted, large-volume (120–1630 L) sampling. Human and bovine viruses and pathogenic bacteria were detected by real-time qPCR in 16%, 14%, and 1.4% of 290 samples collected while protozoa were never detected. The most frequently detected pathogens were: bovine polyomavirus (11%), and human adenovirus C, D, F (9%). Human and bovine viruses were present in 16.9% and 14.8% of runoff-event samples (n = 189) resulting from precipitation and snowmelt, and 13.9% and 12.9% of low-flow samples (n = 101), respectively, indicating multiple delivery mechanisms could be influential. Data indicated human and bovine virus prevalence was different depending on land cover within the watershed. Occurrence, concentration, and flux of human viruses were greatest in samples from the three sampling locations with greater than 25% urban influence than those with less than 25% urban influence. Similarly, occurrence, concentration, and flux of bovine viruses were greatest in samples from the two sampling locations with greater than 50 cattle/km2 than those with less than 50 cattle/km2. In seasonal analysis, human and bovine viruses occurred more frequently in spring and winter seasons than during the fall and summer. Concentration, occurrence, and flux in the context of hydrologic condition, seasonality, and land use must be considered for each watershed individually to develop effective watershed management

  15. The earliest modern mongoose (Carnivora, Herpestidae) from Africa (late Miocene of Chad)

    NASA Astrophysics Data System (ADS)

    Peigné, Stéphane; Bonis, Louis; Likius, Andossa; Mackaye, Hassane Taïsso; Vignaud, Patrick; Brunet, Michel

    2005-06-01

    We report on the earliest modern mongooses of Africa, from the late Miocene (ca. 7 Ma) of the hominid locality TM 266, Toros-Menalla, Chad. The material is based on fragmentary dentitions of three individuals. The main diagnostic feature of the Chadian species is the great development of the shear in the carnassials, which distinguishes the Chadian specimens from all extant herpestids except Herpestes and Galerella. In comparison with most extinct and extant Herpestes, the species from Toros-Menalla differs by a markedly smaller size and, depending on the species, relatively more elongated carnassials, more transversely elongated M1 and more reduced p4. On the basis of a great morphological similarity and the absence of significant differences, we assign our material to Galerella sanguinea; the Chadian finding therefore represents the earliest appearance of an extant species of Herpestidae. This record ties the first appearance of the genus to a minimum age of ca. 7 Ma, which is consistent with the estimated divergence date of 11.4 Ma known from the literature for the species of Galerella.

  16. The earliest modern mongoose (Carnivora, Herpestidae) from Africa (late Miocene of Chad).

    PubMed

    Peigné, Stéphane; de Bonis, Louis; Likius, Andossa; Mackaye, Hassane Taïsso; Vignaud, Patrick; Brunet, Michel

    2005-06-01

    We report on the earliest modern mongooses of Africa, from the late Miocene (ca. 7 Ma) of the hominid locality TM 266, Toros-Menalla, Chad. The material is based on fragmentary dentitions of three individuals. The main diagnostic feature of the Chadian species is the great development of the shear in the carnassials, which distinguishes the Chadian specimens from all extant herpestids except Herpestes and Galerella. In comparison with most extinct and extant Herpestes, the species from Toros-Menalla differs by a markedly smaller size and, depending on the species, relatively more elongated carnassials, more transversely elongated M1 and more reduced p4. On the basis of a great morphological similarity and the absence of significant differences, we assign our material to Galerella sanguinea; the Chadian finding therefore represents the earliest appearance of an extant species of Herpestidae. This record ties the first appearance of the genus to a minimum age of ca. 7 Ma, which is consistent with the estimated divergence date of 11.4 Ma known from the literature for the species of Galerella.

  17. Microplastic pollution in lakes and lake shoreline sediments - A case study on Lake Bolsena and Lake Chiusi (central Italy).

    PubMed

    Fischer, Elke Kerstin; Paglialonga, Lisa; Czech, Elisa; Tamminga, Matthias

    2016-06-01

    Rivers and effluents have been identified as major pathways for microplastics of terrestrial sources. Moreover, lakes of different dimensions and even in remote locations contain microplastics in striking abundances. This study investigates concentrations of microplastic particles at two lakes in central Italy (Lake Bolsena, Lake Chiusi). A total number of six Manta Trawls have been carried out, two of them one day after heavy winds occurred on Lake Bolsena showing effects on particle distribution of fragments and fibers of varying size categories. Additionally, 36 sediment samples from lakeshores were analyzed for microplastic content. In the surface waters 2.68 to 3.36 particles/m(3) (Lake Chiusi) and 0.82 to 4.42 particles/m(3) (Lake Bolsena) were detected, respectively. Main differences between the lakes are attributed to lake characteristics such as surface and catchment area, depth and the presence of local wind patterns and tide range at Lake Bolsena. An event of heavy winds and moderate rainfall prior to one sampling led to an increase of concentrations at Lake Bolsena which is most probable related to lateral land-based and sewage effluent inputs. The abundances of microplastic particles in sediments vary from mean values of 112 (Lake Bolsena) to 234 particles/kg dry weight (Lake Chiusi). Lake Chiusi results reveal elevated fiber concentrations compared to those of Lake Bolsena what might be a result of higher organic content and a shift in grain size distribution towards the silt and clay fraction at the shallow and highly eutrophic Lake Chiusi. The distribution of particles along different beach levels revealed no significant differences. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. The first fossil fungus gardens of Isoptera: oldest evidence of symbiotic termite fungiculture (Miocene, Chad basin)

    NASA Astrophysics Data System (ADS)

    Duringer, Philippe; Schuster, Mathieu; Genise, Jorge F.; Likius, Andossa; Mackaye, Hassan Taisso; Vignaud, Patrick; Brunet, Michel

    2006-12-01

    Higher termites of the subfamily Macrotermitinae (fungus-growing termites) are known to build fungus gardens where a symbiotic fungus ( Termitomyces sp.) is cultivated. The fungus grows on a substrate called fungus comb, a structure built with the termites’ own faeces. Here we present the first fossil fungus combs ever found in the world. They were extracted from 7-million-year-old continental sandstone (Chad basin). Fossilized fungus combs have an ovoid morphology with a more or less flattened concave base and a characteristic general alveolar aspect. Under lens, they display a typical millimetre-scale pelletal structure. The latter, as well as the general shape and alveolar aspect, are similar to the morphology of fungus combs from extant fungus-growing termites.

  19. Approaches to Golden Algae Control: In-Lake Mesocosm Experiments

    DTIC Science & Technology

    2012-04-01

    P. parvum population density (b), and cyanobacteria biomass (c) from monthly sampling trips performed in Lake Granbury where trips spanned five...zooplankton and pathogenic effects of virus (Schwierzke et al. 2010). In addition, some cyanobacteria may inhibit P. parvum blooms (Grover et al...toxins released by P. parvum may behave as weak bases in aqueous solutions. These results suggest that targeted reductions of pH can reduce the

  20. The right to health, health systems development and public health policy challenges in Chad.

    PubMed

    Azétsop, Jacquineau; Ochieng, Michael

    2015-02-15

    There is increasing consensus that the right to health can provide ethical, policy and practical groundings for health systems development. The goals of the right to health are congruent with those of health systems development, which are about strengthening health promotion organizations and actions so as to improve public health. The poor shape and performance of health systems in Chad question the extent of realization of the right to health. Due to its comprehensiveness and inclusiveness, the right to health has the potential of being an organizational and a normative backbone for public health policy and practice. It can then be understood and studied as an integral component of health systems development. This paper uses a secondary data analysis of existing documents by the Ministry of Public Health, Institut National de la Statistique, des Etudes Economiques et Démographiques (INSEED), the Ministry of Economy and Agence Française de Cooperation to analyze critically the shape and performance of health systems in Chad based on key concepts and components of the right to health contained in article 12 of the International Covenant on Economic, Social and Cultural Rights, and on General Comment 14. The non-realization of the right to health, even in a consistently progressive manner, raises concerns about the political commitment of state officials to public health, about the justice of social institutions in ensuring social well-being and about individual and public values that shape decision-making processes. Social justice, democratic rule, transparency, accountability and subsidiarity are important groundings for ensuring community participation in public affairs and for monitoring the performance of public institutions. The normative ideals of health systems development are essentially democratic in nature and are rooted in human rights and in ethical principles of human dignity, equality, non-discrimination and social justice. These ideals are grounded

  1. Lake whitefish and lake herring population structure and niche in ten south-central Ontario lakes

    USGS Publications Warehouse

    Carl, Leon M.; McGuiness, Fiona

    2006-01-01

    This study compares simple fish communities of ten oligotrophic lakes in south-central Ontario. Species densities and population size structure vary significantly among these lake communities depending on fish species present beyond the littoral zone. Lake whitefish are fewer and larger in the presence of lake herring than in their absence. Diet analysis indicates that lake whitefish shift from feeding on both plankton and benthic prey when lake herring are absent to a primarily benthic feeding niche in the presence of lake herring. When benthic round whitefish are present, lake whitefish size and density decline and they move lower in the lake compared to round whitefish. Burbot are also fewer and larger in lakes with lake herring than in lakes without herring. Burbot, in turn, appear to influence the population structure of benthic coregonine species. Lower densities of benthic lake whitefish and round whitefish are found in lakes containing large benthic burbot than in lakes with either small burbot or where burbot are absent. Predation on the pelagic larvae of burbot and lake whitefish by planktivorous lake herring alters the size and age structure of these populations. As life history theory predicts, those species with poor larval survival appear to adopt a bet-hedging life history strategy of long-lived individuals as a reproductive reserve.

  2. Phylogenetic Analysis and Pathogenicity Assessment of Two Strains of Avian Influenza Virus Subtype H9N2 Isolated from Migratory Birds: High Homology of Internal Genes with Human H10N8 Virus.

    PubMed

    Ye, Ge; Liang, Chai Hong; Hua, Deng Guo; Song, Lei Yong; Xiang, Yang Guo; Guang, Chen; Lan, Chen Hua; Ping, Hua Yu

    2016-01-01

    Two human-infecting avian influenza viruses (AIVs), H7N9 and H10N8, have emerged in China, which further indicate that the H9N2 subtype of AIVs, as an internal gene donor, may have an important role in the generation of new viruses with cross-species transmissibility and pathogenicity. H9N2 viruses that contain such internal genes widely exist in poultry but are rarely reported in migratory birds. In this study, two strains of the H9N2 virus were isolated from fecal samples of migratory birds in 2014: one strain from Caizi Lake in Anhui Province and one from Chen Lake in Hubei Province of China. Nucleotide sequence analysis revealed high homology of all six internal genes of these two strains with the internal genes of the human H10N8 virus in Jiangxi Province, as well as with the human H7N9 virus. Phylogenetic analysis indicated a possible origin of these two strains from poultry in South China. Both of the two viruses tested could replicated in respiratory organs of infective mice without adaption, by both strains of the H9N2 AIVs from wild birds, suggesting their potential capacity for directly infecting mammals. Our findings indicate the existence of H9N2 viruses that contain internal genes highly homologous with human H10N8 or H7N9 viruses. Wild birds can contribute to the spread of the H9N2 virus that contains the "harmful" internal gene complex, leading to gene rearrangement with other influenza viruses and to the generation of new pathogenic viruses. Therefore, strengthening AIV surveillance in wild birds can promote an understanding of the presence and prevalence of viruses and provide scientific evidence for the prevention and control of AIVs and human-infecting AIVs.

  3. Seasonal movements and migration of Pallas's Gulls Larus ichthyaetus from Qinghai Lake, China

    USGS Publications Warehouse

    Muzaffar, S.B.; Takekawa, John Y.; Prosser, D.J.; Douglas, David C.; Yan, B.; Xing, Z.; Hou, Y.; Palm, E.C.; Newman, S.H.

    2008-01-01

    We studied the seasonal movements and migration often Pallas's Gulls Larus ichthyaetus trom Qinghai Lake to assess migratory routes and stopover areas. Each individual was captured and equipped with an 18 g solar-powered Platform Transmitter Terminal (PIT) to track its movements from September 2007 to May 2008. Six individuals remained near Qinghai Lake until the PTTs stopped transmitting. Three individuals flew 50-330 km from Qinghai Lake to nearby salt lakes. One individual departed on 8 December and flew over 1,700 km south-west to arrive at coastal Bangladesh on 9 January 2008. Two individuals flew in October to the Brahmaputra River in Assam, India, remaining in the area for at least one month until one stopped transmitting. The second individual travelled southwest to coastal Bangladesh. Of the two individuals overwintering in Bangladesh, one remained for 67 days before migrating north. The second bird departed after 96 days, and it returned to Qinghai on 10 May 2008 after 48 days in migration. Both individuals that overwintered in coastal Bangladesh arrived much later than the outbreaks of Highly Pathogenic Avian Influenza (HP AI H5N I) in poultry in 2007. This disparity in timing would tentatively suggest that this species was not involved in long-distance movements of the virus. Instead, the converse may be true: previous work demonstrates the potential for virus spill-over trom poultry into gulls and other wild bird species upon arrival into locations with widespread HPAI H5NI outbreaks and environmental contamination.

  4. In vitro infection of salmonid epidermal tissues by infectious hematopoietic necrosis virus and viral hemorrhagic septicemia virus

    USGS Publications Warehouse

    Yamamoto, T.; Batts, W.N.; Winton, J.R.

    1992-01-01

    The ability of two rhabdoviruses, infectious hematopoietic necrosis virus (IHNV) and viral hemorrhagic septicemia virus (VHSV), to infect fish skin was investigated by in vitro infection of excised tissues. Virus replication was determined by plaque assay of homogenized tissue extracts, and the virus antigen was detected by immunohistology of tissue sections. Gill, fin, and ventral abdominal skin tissues of rainbow trout Oncorhynchus mykiss that had been infected in vitro with a virulent strain of IHNV (193–110) produced substantial increases in virus titer within 24 h. Titers continued to increase up until day 3 of incubation; by this time, virus had increased 1,000-fold or more. This increase in IHNV titer occurred in epidermal tissues of fingerlings and of older fish. In another experiment, IHNV replicated in excised rainbow trout tissues whether the fish had been subject to prior infection with a virulent strain of IHNV (Western Regional Aquaculture Consortium isolate) or whether the fish had been infected previously with an attenuated strain of the virus (Nan Scott Lake, with 100 passes in culture). A virulent strain of VHSV (23/75) replicated effectively in excised gill tissues and epidermal tissues of rainbow trout and chinook salmon O. tshawytscha; however, the avirulent North American strain of VHSV (Makah) replicated poorly or not at all.

  5. Phylogenetic analysis and molecular methods for the detection of lymphocystis disease virus from yellow perch, Perca flavescens (Mitchell).

    PubMed

    Palmer, L J; Hogan, N S; van den Heuvel, M R

    2012-09-01

    Lymphocystis disease is a prevalent, non-fatal disease that affects many teleost fish and is caused by the DNA virus lymphocystis disease virus (LCDV). Lymphocystis-like lesions have been observed in yellow perch, Perca flavescens (Mitchell), in lakes in northern Alberta, Canada. In an effort to confirm the identity of the virus causing these lesions, DNA was extracted from these lesions and PCR with genotype generic LCDV primers specific to the major capsid protein (MCP) gene was performed. A 1357-base pair nucleotide sequence corresponding to a peptide length of 452 amino acids of the MCP gene was sequenced, confirming the lesions as being lymphocystis disease lesions. Phylogenetic analysis of the generated amino acid sequence revealed the perch LCDV isolate to be a distinct and novel genotype. From the obtained sequence, a real-time PCR identification method was developed using fluorgenic LUX primers. The identification method was used to detect the presence/absence of LCDV in yellow perch from two lakes, one where lymphocystis disease was observed to occur and the other where the disease had not been observed. All samples of fin, spleen and liver tested negative for LCDV in the lake where lymphocystis disease had not been observed. The second lake had a 2.6% incidence of LCD, and virus was detected in tissue samples from all individuals tested regardless of whether they were expressing the disease or not. However, estimated viral copy number in spleen and liver of symptomatic perch was four orders of magnitude higher than that in asymptomatic perch. © 2012 Blackwell Publishing Ltd.

  6. Unacceptably High Mortality Related to Measles Epidemics in Niger, Nigeria, and Chad

    PubMed Central

    Grais, R. F; Dubray, C; Gerstl, S; Guthmann, J. P; Djibo, A; Nargaye, K. D; Coker, J; Alberti, K. P; Cochet, A; Ihekweazu, C; Nathan, N; Payne, L; Porten, K; Sauvageot, D; Schimmer, B; Fermon, F; Burny, M. E; Hersh, B. S; Guerin, P. J

    2007-01-01

    Background Despite the comprehensive World Health Organization (WHO)/United Nations Children's Fund (UNICEF) measles mortality–reduction strategy and the Measles Initiative, a partnership of international organizations supporting measles mortality reduction in Africa, certain high-burden countries continue to face recurrent epidemics. To our knowledge, few recent studies have documented measles mortality in sub-Saharan Africa. The objective of our study was to investigate measles mortality in three recent epidemics in Niamey (Niger), N'Djamena (Chad), and Adamawa State (Nigeria). Methods and Findings We conducted three exhaustive household retrospective mortality surveys in one neighbourhood of each of the three affected areas: Boukoki, Niamey, Niger (April 2004, n = 26,795); Moursal, N'Djamena, Chad (June 2005, n = 21,812); and Dong District, Adamawa State, Nigeria (April 2005, n = 16,249), where n is the total surveyed population in each of the respective areas. Study populations included all persons resident for at least 2 wk prior to the study, a duration encompassing the measles incubation period. Heads of households provided information on measles cases, clinical outcomes up to 30 d after rash onset, and health-seeking behaviour during the epidemic. Measles cases and deaths were ascertained using standard WHO surveillance-case definitions. Our main outcome measures were measles attack rates (ARs) and case fatality ratios (CFRs) by age group, and descriptions of measles complications and health-seeking behaviour. Measles ARs were the highest in children under 5 y old (under 5 y): 17.1% in Boukoki, 17.2% in Moursal, and 24.3% in Dong District. CFRs in under 5-y-olds were 4.6%, 4.0%, and 10.8% in Boukoki, Moursal, and Dong District, respectively. In all sites, more than half of measles cases in children aged under 5 y experienced acute respiratory infection and/or diarrhoea in the 30 d following rash onset. Of measles cases, it was reported that 85.7% (979

  7. West Nile Virus transmission in winter: the 2013 Great Salt Lake Bald Eagle and Eared Grebes Mortality event

    USGS Publications Warehouse

    Ip, Hon S.; Van Wettere, Arnaud J.; McFarlan, Leslie; Shearn-Bochsler, Valerie I.; Dickson, Sammie L.; Baker, JoDee; Hatch, Gary; Cavender, Kimberly; Long, Renee Romaine; Bodenstein, Barbara L.

    2014-01-01

    West Nile Virus (WNV) infection has been reported in over 300 species of birds and mammals. Raptors such as eagles, hawks and falcons are remarkably susceptible, but reports of WNV infection in Bald Eagles (Haliaeetus leucocephalus) are rare and reports of WNV infection in grebes (Podicipediformes) even rarer. We report an unusually large wild bird mortality event involving between 15,000-20,000 Eared Grebes (Podiceps nigricollis) and over 40 Bald Eagles around the Great Salt Lake, Utah, in November-December 2013. Mortality in grebes was first reported in early November during a period when the area was unseasonably warm and the grebes were beginning to gather and stage prior to migration. Ten out of ten Eared Grebes collected during this period were WNV RT-PCR and/or isolation positive. This is the first report of WNV infection in Eared Grebes and the associated mortality event is matched in scale only by the combined outbreaks in American White Pelican (Pelecanus erythrorhynchos) colonies in the north central states in 2002-2003. We cannot be sure that all of the grebes were infected by mosquito transmission; some may have become infected through contact with WNV shed orally or cloacally from other infected grebes. Beginning in early December, Bald Eagles in the Great Salt Lake area were observed to display neurological signs such as body tremors, limb paralysis and lethargy. At least 43 Bald Eagles had died by the end of the month. Nine of nine Bald Eagles examined were infected with WNV. To the best of our knowledge, this is the largest single raptor mortality event since WNV became endemic in the USA. Because the majority of the eagles affected were found after onset of below-freezing temperatures, we suggest at least some of the Bald Eagles were infected with WNV via consumption of infected Eared Grebes or horizontal transmission at roost sites.

  8. The occurrence of lysogenic bacteria and microbial aggregates in the lakes of the McMurdo Dry Valleys, Antarctica

    USGS Publications Warehouse

    Lisle, J.T.; Priscu, J.C.

    2004-01-01

    The McMurdo Dry Valleys of Antarctica form the coldest and driest ecosystem on Earth. Within this region there are a number of perennially ice-covered (3-6 m thick) lakes that support active microbial assemblages and have a paucity of metazoans. These lakes receive limited allochthonous input of carbon and nutrients, and primary productivity is limited to only 6 months per year owing to an absence of sunlight during the austral winters. In an effort to establish the role that bacteria and their associated viruses play in carbon and nutrient cycling in these lakes, indigenous bacteria, free bacteriophage, and lysogen abundances were determined. Total bacterial abundances (TDC) ranged from 3.80 ?? 104 to 2.58 ?? 107 cells mL-1 and virus-like particle (VLP) abundances ranged from 2.26 ?? 105 to 5.56 ?? 107 VLP mL-1. VLP abundances were significantly correlated (P < 0.05) with TDC, bacterial productivity (TdR), chlorophyll a (Chl a), and soluble reactive phosphorus (SRP). Lysogenic bacteria, determined by induction with mitomycin C, made up between 2.0% and 62.5% of the total population of bacteria when using significant decreases and increases in TDC and VLP abundances, respectively, and 89.5% when using increases in VLP abundances as the sole criterion for a successful induction event. The contribution of viruses released from induced lysogens contributed <0.015% to the total viral production rate. Carbohydrate and protein based organic aggregates were abundant within the water column of the lakes and were heavily colonized by bacteria and VLPs. Alkaline phosphatase activity was detected within the matrix of the aggregates, implying phosphorus deficiency and consortial nutrient exchanges among microorganisms.

  9. Cle Elum Lake Sockeye Salmon Restoration Feasibility Study, 1987-1989 Progress Report.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Flagg, Thomas A.

    1990-02-01

    This report summarizes research activities conducted by the National Marine Fisheries Service (NMFS) from July 1988 through March 1989 relating to the Cle Elum Lake sockeye salmon restoration feasibility study. During this period, efforts focused on collection and spawning of adult sockeye salmon from the Wenatchee River, incubation of eggs from the 1988-brood, and the rearing of juveniles from the 1987-brood. In late July and early August 1988, 520 adult sockeye salmon were captured at fishways on the Wenatchee River and transferred to net-pens in Lake Wenatchee. Fish were held to maturity in late September and early October, spawned, andmore » eggs incubated at a quarantine hatchery in Seattle, WA. The 336 sockeye salmon successfully spawned from the net-pens at Lake Wenatchee were surveyed for the presence of infectious hematopoietic necrosis (IHN) and other replicating viruses. In addition, 13 and 5 sockeye salmon spawners were surveyed from spawning grounds on the White and Little Wenatchee Rivers, respectively, from within the Lake Wenatchee system. 12 refs., 4 figs., 6 tabs.« less

  10. Incidence of canine rabies in N'Djaména, Chad.

    PubMed

    Kayali, U; Mindekem, R; Yémadji, N; Oussiguéré, A; Naïssengar, S; Ndoutamia, A G; Zinsstag, J

    2003-11-12

    This work describes for the first time the incidence risk of passively reported canine rabies, and quantifies reported human exposure in N'Djaména (the capital of Chad). To diagnose rabies, we used a direct immunofluorescent-antibody test (IFAT). From January 2001 to March 2002, we were brought 34 rabies cases in dogs and three cases in cats. Canine cases were geographically clustered. The annual incidence risk of canine rabies was 1.4 (95% CI: 1.2, 1.7) per 1000 unvaccinated dogs. Most of the rabid dogs were owned-although free-roaming and not vaccinated against rabies. Most showed increased aggressiveness and attacked people without being provoked. Eighty-one persons were exposed to rabid dogs and four persons to rabid cats (mostly children<15 years old). Most of the exposed persons were neighbours or family members of the animal owner. Most exposures were transdermal bites, but nearly half of all exposed persons did not apply any first wound care or only applied a traditional treatment. In N'Djaména, humans are often exposed to canine rabies but do not use the full-course post-exposure treatment and wound care is insufficient. Most rabid dogs would be accessible to parenteral vaccination. Pilot vaccination campaigns are needed to determine the success of dog mass vaccination in N'Djaména as a way to prevent animal and human rabies.

  11. Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia.

    PubMed

    Bacharach, Eran; Mishra, Nischay; Briese, Thomas; Zody, Michael C; Kembou Tsofack, Japhette Esther; Zamostiano, Rachel; Berkowitz, Asaf; Ng, James; Nitido, Adam; Corvelo, André; Toussaint, Nora C; Abel Nielsen, Sandra Cathrine; Hornig, Mady; Del Pozo, Jorge; Bloom, Toby; Ferguson, Hugh; Eldar, Avi; Lipkin, W Ian

    2016-04-05

    Tilapia are an important global food source due to their omnivorous diet, tolerance for high-density aquaculture, and relative disease resistance. Since 2009, tilapia aquaculture has been threatened by mass die-offs in farmed fish in Israel and Ecuador. Here we report evidence implicating a novel orthomyxo-like virus in these outbreaks. The tilapia lake virus (TiLV) has a 10-segment, negative-sense RNA genome. The largest segment, segment 1, contains an open reading frame with weak sequence homology to the influenza C virus PB1 subunit. The other nine segments showed no homology to other viruses but have conserved, complementary sequences at their 5' and 3' termini, consistent with the genome organization found in other orthomyxoviruses. In situ hybridization indicates TiLV replication and transcription at sites of pathology in the liver and central nervous system of tilapia with disease. The economic impact of worldwide trade in tilapia is estimated at $7.5 billion U.S. dollars (USD) annually. The infectious agent implicated in mass tilapia die-offs in two continents poses a threat to the global tilapia industry, which not only provides inexpensive dietary protein but also is a major employer in the developing world. Here we report characterization of the causative agent as a novel orthomyxo-like virus, tilapia lake virus (TiLV). We also describe complete genomic and protein sequences that will facilitate TiLV detection and containment and enable vaccine development. Copyright © 2016 Bacharach et al.

  12. Highly pathogenic influenza A(H5N1) virus survival in complex artificial aquatic biotopes.

    PubMed

    Horm, Viseth Srey; Gutiérrez, Ramona A; Nicholls, John M; Buchy, Philippe

    2012-01-01

    Very little is known regarding the persistence of Highly Pathogenic Avian Influenza (HPAI) H5N1 viruses in aquatic environments in tropical countries, although environmental materials have been suggested to play a role as reservoirs and sources of transmission for H5N1 viruses. The survival of HPAI H5N1 viruses in experimental aquatic biotopes (water, mud, aquatic flora and fauna) relevant to field conditions in Cambodia was investigated. Artificial aquatic biotopes, including simple ones containing only mud and water, and complex biotopes involving the presence of aquatic flora and fauna, were set up. They were experimentally contaminated with H5N1 virus. The persistence of HPAI H5N1 virus (local avian and human isolates) was determined by virus isolation in embryonated chicken eggs and by real-time reverse-polymerase chain reaction. Persistence of infectious virus did not exceed 4 days, and was only identified in rain water. No infectious virus particles were detected in pond and lake water or mud even when high inoculum doses were used. However, viral RNA persisted up to 20 days in rain water and 7 days in pond or lake water. Viral RNA was also detected in mud samples, up to 14 days post-contamination in several cases. Infectious virus and viral RNA was detected in few cases in the aquatic fauna and flora, especially in bivalves and labyrinth fish, although these organisms seemed to be mostly passive carriers of the virus rather than host allowing virus replication. Although several factors for the survival and persistence of HPAI viruses in the environment are still to be elucidated, and are particularly hard to control in laboratory conditions, our results, along with previous data, support the idea that environmental surveillance is of major relevance for avian influenza control programs.

  13. Highly Pathogenic Influenza A(H5N1) Virus Survival in Complex Artificial Aquatic Biotopes

    PubMed Central

    Horm, Viseth Srey; Gutiérrez, Ramona A.; Nicholls, John M.; Buchy, Philippe

    2012-01-01

    Background Very little is known regarding the persistence of Highly Pathogenic Avian Influenza (HPAI) H5N1 viruses in aquatic environments in tropical countries, although environmental materials have been suggested to play a role as reservoirs and sources of transmission for H5N1 viruses. Methodology/Principal Findings The survival of HPAI H5N1 viruses in experimental aquatic biotopes (water, mud, aquatic flora and fauna) relevant to field conditions in Cambodia was investigated. Artificial aquatic biotopes, including simple ones containing only mud and water, and complex biotopes involving the presence of aquatic flora and fauna, were set up. They were experimentally contaminated with H5N1 virus. The persistence of HPAI H5N1 virus (local avian and human isolates) was determined by virus isolation in embryonated chicken eggs and by real-time reverse-polymerase chain reaction. Persistence of infectious virus did not exceed 4 days, and was only identified in rain water. No infectious virus particles were detected in pond and lake water or mud even when high inoculum doses were used. However, viral RNA persisted up to 20 days in rain water and 7 days in pond or lake water. Viral RNA was also detected in mud samples, up to 14 days post-contamination in several cases. Infectious virus and viral RNA was detected in few cases in the aquatic fauna and flora, especially in bivalves and labyrinth fish, although these organisms seemed to be mostly passive carriers of the virus rather than host allowing virus replication. Conclusions/Significance Although several factors for the survival and persistence of HPAI viruses in the environment are still to be elucidated, and are particularly hard to control in laboratory conditions, our results, along with previous data, support the idea that environmental surveillance is of major relevance for avian influenza control programs. PMID:22514622

  14. 2016 Lake Michigan Lake Trout Working Group Report

    USGS Publications Warehouse

    Madenjian, Charles P.; Breidert, Brian; Boyarski, David; Bronte, Charles R.; Dickinson, Ben; Donner, Kevin; Ebener, Mark P.; Gordon, Roger; Hanson, Dale; Holey, Mark; Janssen, John; Jonas, Jory; Kornis, Matthew; Olsen, Erik; Robillard, Steve; Treska, Ted; Weldon, Barry; Wright, Greg D.

    2017-01-01

    This report provides a review on the progression of lake trout rehabilitation towards meeting the Salmonine Fish Community Objectives (FCOs) for Lake Michigan (Eshenroder et. al. 1995) and the interim goal and evaluation objectives articulated in A Fisheries Management Implementation Strategy for the Rehabilitation of Lake Trout in Lake Michigan (Dexter et al. 2011); we also include data describing lake trout stocking and mortality to portray the present state of progress towards lake trout rehabilitation.

  15. Contaminants in American alligator eggs from Lake Apopka, Lake Griffin, and Lake Okeechobee, Florida

    USGS Publications Warehouse

    Heinz, Gary H.; Percival, H. Franklin; Jennings, Michael L.

    1991-01-01

    Residues of organochlorine pesticides, polychlorinated biphenyls (PCBs), and 16 elements were measured in American alligator (Alligator mississippiensis) eggs collected in 1984 from Lakes Apopka, Griffin, and Okeechobee in central and south Florida. Organochlorine pesticides were highest in eggs from Lake Apopka. None of the elements appeared to be present at harmful concentrations in eggs from any of the lakes. A larger sample of eggs was collected in 1985, but only from Lakes Griffin, a lake where eggs were relatively clean, and Apopka, where eggs were most contaminated. In 1985, hatching success of artificially incubated eggs was lower for Lake Apopka, and several organochlorine pesticides were higher than in eggs from Lake Griffin. However, within Lake Apopka, higher levels of pesticides in chemically analyzed eggs were not associated with reduced hatching success of the remaining eggs in the clutch. Therefore, it did not appear that any of the pesticides we measured were responsible for the reduced hatching success of Lake Apopka eggs.

  16. REMOVAL OF HEPATITIS A VIRUS AND ROTAVIRUS BY DRINKING WATER TREATMENT

    EPA Science Inventory

    The paper presents quantitative data from a two year study on the removability of rotavirus SA11 and hepatitis A virus added exogenously to Lake Houston raw water during treatment. Processes studied on laboratory and pilot scale included coagulation, filtration, softening and dis...

  17. Sanctuaries for lake trout in the Great Lakes

    USGS Publications Warehouse

    Stanley, Jon G.; Eshenroder, Randy L.; Hartman, Wilbur L.

    1987-01-01

    Populations of lake trout, severely depleted in Lake Superior and virtually extirpated from the other Great Lakes because of sea lamprey predation and intense fishing, are now maintained by annual plantings of hatchery-reared fish in Lakes Michigan, Huron, and Ontario and parts of Lake Superior. The extensive coastal areas of the Great Lakes and proximity to large populations resulted in fishing pressure on planted lake trout heavy enough to push annual mortality associated with sport and commercial fisheries well above the critical level needed to reestablish self-sustaining stocks. The interagency, international program for rehabilitating lake trout includes controlling sea lamprey abundance, stocking hatchery-reared lake trout, managing the catch, and establishing sanctuaries where harvest is prohibited. Three lake trout sanctuaries have been established in Lake Michigan: the Fox Island Sanctuary of 121, 500 ha, in the Chippewa-Ottawa Treaty fishing zone in the northern region of the lake; the Milwaukee Reef Sanctuary of 160, 000 ha in midlake, in boundary waters of Michigan and Wisconsin; and Julian's Reef Sanctuary of 6, 500 ha, in Illinois waters. In northern Lake Huron, Drummond Island Sanctuary of 55, 000 ha is two thirds in Indian treaty-ceded waters in Michigan and one third in Ontario waters of Canada. A second sanctuary, Six Fathom Bank-Yankee Reef Sanctuary, in central Lake Huron contains 168, 000 ha. Sanctuary status for the Canadian areas remains to be approved by the Provincial government. In Lake Superior, sanctuaries protect the spawning grounds of Gull Island Shoal (70, 000 ha) and Devils Island Shoal (44, 000 ha) in Wisconsin's Apostle Island area. These seven sanctuaries, established by the several States and agreed upon by the States, Indian tribes, the U.S. Department of the Interior, and the Province of Ontario, contribute toward solving an interjurisdictional fishery problem.

  18. Geodermatophilus saharensis sp. nov., isolated from sand of the Saharan desert in Chad.

    PubMed

    Montero-Calasanz, M C; Göker, M; Pötter, G; Rohde, M; Spröer, C; Schumann, P; Gorbushina, A A; Klenk, H-P

    2013-03-01

    A novel Gram-positive, aerobic, actinobacterial strain, CF5/5, was isolated from soil in the Sahara desert, Chad. It grew best at 20-35 °C and at pH 6.0-8.0 and with 0-4 % (w/v) NaCl, forming black-colored colonies. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The DNA G + C content was 75.9 mol%. The peptidoglycan contained meso-diaminopimelic acid; galactose and xylose were detected as diagnostic sugars. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, and phosphatidylinositol; MK-9(H(4)) was the dominant menaquinone. The major cellular fatty acids were: iso-C(16:0) and iso-C(15:0). The 16S rRNA gene showed 95.6-98.3 % sequence similarity with the other named members of the genus Geodermatophilus. Based on the polyphasic taxonomy data, the isolate is proposed to represent a novel species, Geodermatophilus saharensis with the type strain CF5/5(T) = DSM 45423 = CCUG 62813 = MTCC 11416.

  19. Water quality of Lake Austin and Town Lake, Austin, Texas

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Andrews, F.L.; Wells, F.C.; Shelby, W.J.

    1988-01-01

    Lake Austin and Town Lake are impoundments on the Colorado River in Travis County, central Texas, and are a source of water for municipal industrial water supplies, electrical-power generation, and recreation for more than 500,000 people in the Austin metropolitan area. Small vertical temperature variations in both lakes were attributed to shallow depths in the lakes and short retention times of water in the lakes during the summer months. The largest areal variations in dissolved oxygen generally occur in Lake Austin during the summer as a result of releases of water from below the thermocline in Lake Travis. Except formore » iron, manganese, and mercury, dissolved concentrations of trace elements in water collected from Lake Austin and Town Lake did not exceed the primary or secondary drinking water standards set by the US Environmental Protection Agency. Little or no effect of stormwater runoff on temperature, dissolved oxygen, or minor elements could be detected in either Lake Austin or Town Lake. Little seasonal or areal variation was noted in nitrogen concentrations in Lake Austin or Town lake. Total phosphorus concentrations generally were small in both lakes. Increased concentrations of nitrogen and phosphorus were detected after storm runoff inflow in Town Lake, but not in Lake Austin; densities of fecal-coliform bacteria increased in Lake Austin and Town Lake, but were substantially greater in Town Lake than in Lake Austin. 18 refs., 38 figs., 59 tabs.« less

  20. Viruses Occur Incorporated in Biogenic High-Mg Calcite from Hypersaline Microbial Mats

    PubMed Central

    De Wit, Rutger; Gautret, Pascale; Bettarel, Yvan; Roques, Cécile; Marlière, Christian; Ramonda, Michel; Nguyen Thanh, Thuy; Tran Quang, Huy; Bouvier, Thierry

    2015-01-01

    Using three different microscopy techniques (epifluorescence, electronic and atomic force microscopy), we showed that high-Mg calcite grains in calcifying microbial mats from the hypersaline lake “La Salada de Chiprana”, Spain, contain viruses with a diameter of 50–80 nm. Energy-dispersive X-ray spectrometer analysis revealed that they contain nitrogen and phosphorus in a molar ratio of ~9, which is typical for viruses. Nucleic acid staining revealed that they contain DNA or RNA. As characteristic for hypersaline environments, the concentrations of free and attached viruses were high (>1010 viruses per g of mat). In addition, we showed that acid treatment (dissolution of calcite) resulted in release of viruses into suspension and estimated that there were ~15 × 109 viruses per g of calcite. We suggest that virus-mineral interactions are one of the possible ways for the formation of nano-sized structures often described as “nanobacteria” and that viruses may play a role in initiating calcification. PMID:26115121

  1. A survey of North American migratory waterfowl for duck plague (duck virus enteritis) virus

    USGS Publications Warehouse

    Brand, Christopher J.; Docherty, Douglas E.

    1984-01-01

    A survey of migratory waterfowl for duck plague (DP) virus was conducted in the Mississippi and Central flyways during 1982 and in the Atlantic and Pacific flyways during 1983. Cloacal and pharyngeal swabs were collected from 3,169 migratory waterfowl in these four flyways, principally mallards (Anas platyrhynchos L.), black ducks (Anas rubripes Brewster), and pintails (Anas acuta L). In addition 1,033 birds were sampled from areas of recurrent DP outbreaks among nonmigratory and captive waterfowl, and 590 from Lake Andes National Wildlife Refuge, the site of the only known major DP outbreak in migratory waterfowl. Duck plague virus was not found in any of the samples. Results support the hypothesis that DP is not established in North American migratory waterfowl as an enzootic disease.

  2. Evidence of offshore lake trout reproduction in Lake Huron

    USGS Publications Warehouse

    DeSorcie, Timothy J.; Bowen, Charles A.

    2003-01-01

    Six Fathom Bank-Yankee Reef, an offshore reef complex, was an historically important spawning area believed to represent some of the best habitat for the rehabilitation of lake trout Salvelinus namaycush in Lake Huron. Since 1986, lake trout have been stocked on these offshore reefs to reestablish self-sustaining populations. We sampled with beam trawls to determine the abundance of naturally reproduced age-0 lake trout on these offshore reefs during May-July in 1994-1998 and 2000-2002. In total, 123 naturally reproduced lake trout fry were caught at Six Fathom Bank, and 2 naturally reproduced lake trout fry were caught at nearby Yankee Reef. Our findings suggest that this region of Lake Huron contains suitable habitat for lake trout spawning and offers hope that lake trout rehabilitation can be achieved in the main basin of Lake Huron.

  3. Lake Nasser and Toshka Lakes, Egypt

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Lake Nasser (center) and the Toshka Lakes (center left) glow emerald green and black in this MODIS true-color image acquired March 8, 2002. Located on and near the border of Egypt and Norther Sudan, these lakes are an oasis of water in between the Nubian (lower right) and Libyan Deserts (upper left). Also visible are the Red Sea (in the upper right) and the Nile River (running north from Lake Nasser). Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  4. Predictive ability of the CHADS2 and CHA2DS2-VASc scores for stroke after transcatheter aortic balloon-expandable valve implantation: an Italian Transcatheter Balloon-Expandable Valve Implantation Registry (ITER) sub-analysis.

    PubMed

    Conrotto, Federico; D'Ascenzo, Fabrizio; D'Onofrio, Augusto; Agrifoglio, Marco; Chieffo, Alaide; Cioni, Micaela; Regesta, Tommaso; Tarantini, Giuseppe; Gabbieri, Davide; Saia, Francesco; Tamburino, Corrado; Ribichini, Flavio; Cugola, Diego; Aiello, Marco; Sanna, Francesco; Iadanza, Alessandro; Pompei, Esmeralda; Stolcova, Miroslava; Cappai, Antioco; Minati, Alessandro; Cassese, Mauro; Martinelli, Gian Luca; Agostinelli, Andrea; Gerosa, Gino; Gaita, Fiorenzo; Rinaldi, Mauro; Salizzoni, Stefano

    2016-11-01

    Stroke incidence after transcatheter aortic valve implantation (TAVI) still represents a concern. This multicentre study aimed at investigating the hypothesis that CHADS2 and CHA2DS2-VASc scores may be used to predict perioperative stroke after TAVI. The Italian Transcatheter Balloon-Expandable Valve Implantation Registry (ITER) is a multicentre, prospective registry of patients undergoing balloon-expandable TAVI using Edwards Sapien and Sapien XT prosthesis between 2007 and 2012. The primary end-point of this study was the 30-day stroke rate. Secondary safety end-points were all the major adverse events based on Valve Academic Research Consortium (VARC-2) criteria. One thousand nine hundred and four patients were enrolled in the registry. Mean age was 81.6 ± 6.2 years and 1147 (60.2%) patients were female; mean CHADS 2 and CHA 2 DS 2 -VASc scores were 2.2 ± 0.8 and 4.4 ± 1.1, respectively. Fifty-four (2.8%) patients had a stroke within 30 days. At multivariable logistic regression analysis, CHA 2 DS 2 -VASc (OR: 1.35, 95% CI: 1.03-1.78; P = 0.031) and previous cardiac surgery (OR: 1.96, 95% CI: 1.06-3.6; P = 0.033) but not CHADS 2 (OR: 1.05, 95% CI: 0.76-1.44; P = 0.77) were found to be independent predictors of in-hospital stroke. A CHA 2 DS 2 -VASc score ≥5 was strongly related to the occurrence of in-hospital stroke (OR: 2.51, 95% CI: 1.38-4.57; P= 0.001). However, CHA 2 DS 2 -VASc score showed only poor accuracy for in-hospital stroke with a trend for better accuracy when compared with CHADS 2 score (area under the curve: 0.61, 95% CI: 0.59-0.63 vs 0.51; 95% CI: 0.49-0.54, respectively, P = 0.092). In TAVI patients, CHA 2 DS 2 -VASc provided a strong correlation for in-hospital stroke but with low accuracy. Dedicated scores to properly tailor procedures and preventive strategies are needed. © The Author 2016. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved.

  5. The role of solar UV radiation in the ecology of alpine lakes.

    PubMed

    Sommaruga, R

    2001-09-01

    Solar ultraviolet radiation (UVR, 290-400 nm) is a crucial environmental factor in alpine lakes because of the natural increase of the UVR flux with elevation and the high water transparency of these ecosystems. The ecological importance of UVR, however, has only recently been recognized. This review, examines the general features of alpine lakes regarding UVR, summarizes what is known about the role of solar UVR in the ecology of alpine lakes, and identifies future research directions. Unlike the pattern observed in most lowland lakes, variability of UV attenuation in alpine lakes is poorly explained by differences in dissolved organic carbon (DOC) concentrations, and depends mainly on optical characteristics (absorption) of the chromophoric dissolved organic matter (CDOM). Within the water column of lakes with low DOC concentrations (0.2-0.4 mg l(-1)), UV attenuation is influenced by phytoplankton whose development at depth (i.e. the deep chlorophyll maximum) causes important changes in UV attenuation. Alpine aquatic organisms have developed a number of strategies to minimize UV damage. The widespread synthesis or bioaccumulation of different compounds that directly or indirectly absorb UV energy is one such strategy. Although most benthic and planktonic primary producers and crustacean zooplankton are well adapted to high intensities of solar radiation, heterotrophic protists, bacteria, and viruses seem to be particularly sensitive to UVR. Understanding the overall impact of UVR on alpine lakes would need to consider synergistic and antagonistic processes resulting from the pronounced climatic warming, which have the potential to modify the UV underwater climate and consequently the stress on aquatic organisms.

  6. Lake whitefish diet, condition, and energy density in Lake Champlain and the lower four Great Lakes following dreissenid invasions

    USGS Publications Warehouse

    Herbst, Seth J.; Marsden, J. Ellen; Lantry, Brian F.

    2013-01-01

    Lake Whitefish Coregonus clupeaformis support some of the most valuable commercial freshwater fisheries in North America. Recent growth and condition decreases in Lake Whitefish populations in the Great Lakes have been attributed to the invasion of the dreissenid mussels, zebra mussels Dreissena polymorpha and quagga mussels D. bugensis, and the subsequent collapse of the amphipod, Diporeia, a once-abundant high energy prey source. Since 1993, Lake Champlain has also experienced the invasion and proliferation of zebra mussels, but in contrast to the Great Lakes, Diporeia were not historically abundant. We compared the diet, condition, and energy density of Lake Whitefish from Lake Champlain after the dreissenid mussel invasion to values for those of Lake Whitefish from Lakes Michigan, Huron, Erie, and Ontario. Lake Whitefish were collected using gill nets and bottom trawls, and their diets were quantified seasonally. Condition was estimated using Fulton's condition factor (K) and by determining energy density. In contrast to Lake Whitefish from some of the Great Lakes, those from Lake Champlain Lake Whitefish did not show a dietary shift towards dreissenid mussels, but instead fed primarily on fish eggs in spring, Mysis diluviana in summer, and gastropods and sphaeriids in fall and winter. Along with these dietary differences, the condition and energy density of Lake Whitefish from Lake Champlain were high compared with those of Lake Whitefish from Lakes Michigan, Huron, and Ontario after the dreissenid invasion, and were similar to Lake Whitefish from Lake Erie; fish from Lakes Michigan, Huron, and Ontario consumed dreissenids, whereas fish from Lake Erie did not. Our comparisons of Lake Whitefish populations in Lake Champlain to those in the Great Lakes indicate that diet and condition of Lake Champlain Lake Whitefish were not negatively affected by the dreissenid mussel invasion.

  7. Investigation of avian influenza infections in wild birds, poultry and humans in Eastern Dongting Lake, China.

    PubMed

    Shi, Jinghong; Gao, Lidong; Zhu, Yun; Chen, Tao; Liu, Yunzhi; Dong, Libo; Liu, Fuqiang; Yang, Hao; Cai, Yahui; Yu, Mingdong; Yao, Yi; Xu, Cuilin; Xiao, Xiangming; Shu, Yuelong

    2014-01-01

    We investigated avian influenza infections in wild birds, poultry, and humans at Eastern Dongting Lake, China. We analyzed 6,621 environmental samples, including fresh fecal and water samples, from wild birds and domestic ducks that were collected from the Eastern Dongting Lake area from November 2011 to April 2012. We also conducted two cross-sectional serological studies in November 2011 and April 2012, with 1,050 serum samples collected from people exposed to wild birds and/or domestic ducks. Environmental samples were tested for the presence of avian influenza virus (AIV) using quantitative PCR assays and virus isolation techniques. Hemagglutination inhibition assays were used to detect antibodies against AIV H5N1, and microneutralization assays were used to confirm these results. Among the environmental samples from wild birds and domestic ducks, AIV prevalence was 5.19 and 5.32%, respectively. We isolated 39 and 5 AIVs from the fecal samples of wild birds and domestic ducks, respectively. Our analysis indicated 12 subtypes of AIV were present, suggesting that wild birds in the Eastern Dongting Lake area carried a diverse array of AIVs with low pathogenicity. We were unable to detect any antibodies against AIV H5N1 in humans, suggesting that human infection with H5N1 was rare in this region.

  8. Spatial patterns in PCB concentrations of Lake Michigan lake trout

    USGS Publications Warehouse

    Madenjian, Charles P.; DeSorcie, Timothy J.; Stedman, Ralph M.; Brown, Edward H.; Eck, Gary W.; Schmidt, Larry J.; Hesselberg, Robert J.; Chernyak, Sergei M.; Passino-Reader, Dora R.

    1999-01-01

    Most of the PCB body burden in lake trout (Salvelinus namaycush) of the Great Lakes is from their food. PCB concentrations were determined in lake trout from three different locations in Lake Michigan during 1994–1995, and lake trout diets were analyzed at all three locations. The PCB concentrations were also determined in alewife (Alosa pseudoharengus), rainbow smelt (Osmerus mordax), bloater (Coregonus hoyi), slimy sculpin (Cottus cognatus), and deepwater sculpin (Myoxocephalus thompsoni), five species of prey fish eaten by lake trout in Lake Michigan, at three nearshore sites in the lake. Despite the lack of significant differences in the PCB concentrations of alewife, rainbow smelt, bloater, slimy sculpin, and deepwater sculpin from the southeastern nearshore site near Saugatuck (Michigan) compared with the corresponding PCB concentrations from the northwestern nearshore site near Sturgeon Bay (Wisconsin), PCB concentrations in lake trout at Saugatuck were significantly higher than those at Sturgeon Bay. The difference in the lake trout PCB concentrations between Saugatuck and Sturgeon Bay could be explained by diet differences. The diet of lake trout at Saugatuck was more concentrated in PCBs than the diet of Sturgeon Bay lake trout, and therefore lake trout at Saugatuck were more contaminated in PCBs than Sturgeon Bay lake trout. These findings were useful in interpreting the long-term monitoring series for contaminants in lake trout at both Saugatuck and the Wisconsin side of the lake.

  9. Potential strategies for recovery of lake whitefish and lake herring stocks in eastern Lake Erie

    USGS Publications Warehouse

    Oldenburg, K.; Stapanian, M.A.; Ryan, P.A.; Holm, E.

    2007-01-01

    Lake Erie sustained large populations of ciscoes (Salmonidae: Coregoninae) 120 years ago. By the end of the 19th century, abundance of lake whitefish (Coregonus clupeaformis) had declined drastically. By 1925, the lake herring (a cisco) population (Coregonus artedii) had collapsed, although a limited lake herring fishery persisted in the eastern basin until the 1950s. In the latter part of the 20th century, the composition of the fish community changed as oligotrophication proceeded. Since 1984, a limited recovery of lake whitefish has occurred, however no recovery was evident for lake herring. Current ecological conditions in Lake Erie probably will not inhibit recovery of the coregonine species. Recovery of walleye (Sander vitreus) and efforts to rehabilitate the native lake trout (Salvelinus namaycush) in Lake Erie will probably assist recovery because these piscivores reduce populations of alewife (Alosa psuedoharengus) and rainbow smelt (Osmerus mordax), which inhibit reproductive success of coregonines. Although there are considerable spawning substrates available to coregonine species in eastern Lake Erie, eggs and fry would probably be displaced by storm surge from most shoals. Site selection for stocking or seeding of eggs should consider the reproductive life cycle of the stocked fish and suitable protection from storm events. Two potential sites in the eastern basin have been identified. Recommended management procedures, including commercial fisheries, are suggested to assist in recovery. Stocking in the eastern basin of Lake Erie is recommended for both species, as conditions are adequate and the native spawning population in the eastern basin is low. For lake herring, consideration should be given to match ecophenotypes as much as possible. Egg seeding is recommended. Egg seeding of lake whitefish should be considered initially, with fingerling or yearling stocking suggested if unsuccessful. Spawning stocks of whitefish in the western basin of Lake

  10. Glacial lake inventory and lake outburst potential in Uzbekistan.

    PubMed

    Petrov, Maxim A; Sabitov, Timur Y; Tomashevskaya, Irina G; Glazirin, Gleb E; Chernomorets, Sergey S; Savernyuk, Elena A; Tutubalina, Olga V; Petrakov, Dmitriy A; Sokolov, Leonid S; Dokukin, Mikhail D; Mountrakis, Giorgos; Ruiz-Villanueva, Virginia; Stoffel, Markus

    2017-08-15

    Climate change has been shown to increase the number of mountain lakes across various mountain ranges in the World. In Central Asia, and in particular on the territory of Uzbekistan, a detailed assessment of glacier lakes and their evolution over time is, however lacking. For this reason we created the first detailed inventory of mountain lakes of Uzbekistan based on recent (2002-2014) satellite observations using WorldView-2, SPOT5, and IKONOS imagery with a spatial resolution from 2 to 10m. This record was complemented with data from field studies of the last 50years. The previous data were mostly in the form of inventories of lakes, available in Soviet archives, and primarily included localized in-situ data. The inventory of mountain lakes presented here, by contrast, includes an overview of all lakes of the territory of Uzbekistan. Lakes were considered if they were located at altitudes above 1500m and if lakes had an area exceeding 100m 2 . As in other mountain regions of the World, the ongoing increase of air temperatures has led to an increase in lake number and area. Moreover, the frequency and overall number of lake outburst events have been on the rise as well. Therefore, we also present the first outburst assessment with an updated version of well-known approaches considering local climate features and event histories. As a result, out of the 242 lakes identified on the territory of Uzbekistan, 15% are considered prone to outburst, 10% of these lakes have been assigned low outburst potential and the remainder of the lakes have an average level of outburst potential. We conclude that the distribution of lakes by elevation shows a significant influence on lake area and hazard potential. No significant differences, by contrast, exist between the distribution of lake area, outburst potential, and lake location with respect to glaciers by regions. Copyright © 2017 Elsevier B.V. All rights reserved.

  11. Progress toward lake trout restoration in Lake Michigan

    USGS Publications Warehouse

    Holey, Mark E.; Rybicki, Ronald W.; Eck, Gary W.; Brown, Edward H.; Marsden, J. Ellen; Lavis, Dennis S.; Toneys, Michael L.; Trudeau, Tom N.; Horrall, Ross M.

    1995-01-01

    Progress toward lake trout restoration in Lake Michigan is described through 1993. Extinction of the native lake trout fishery by sea lamprey predation, augmented by exploitation and habitat destruction, resulted in an extensive stocking program of hatchery-reared lake trout that began in 1965. Sea lamprey abundance was effectively controlled using selective chemical toxicants. The initial stocking produced a measurable wild year class of lake trout by 1976 in Grand Traverse Bay, but failed to continue probably due to excessive exploitation. The overall lack of successful reproduction lakewide by the late 1970s led to the development and implementation in 1985 of a focused inter-agency lakewide restoration plan by a technical committee created through the Lake Committee structure of the Great Lakes Fishery Commission. Strategies implemented in 1985 by the plan included setting a 40% total mortality goal lakewide, creating two large refuges designed to encompass historically the most productive spawning habitat and protect trout stocked over their home range, evaluating several lake trout strains, and setting stocking priorities throughout the lake. Target levels for stocking in the 1985 Plan have never been reached, and are much less than the estimated lakewide recruitment of yearlings by the native lake trout stocks. Since 1985, over 90% of the available lake trout have been stocked over the best spawning habitat, and colonization of the historically productive offshore reefs has occurred. Concentrations of spawning lake trout large enough for successful reproduction, based on observations of successful hatchery and wild stocks, have developed at specific reefs. Continued lack of recruitment at these specific sites suggests that something other than stotk abundance has limited success. Poor survival of lake trout eggs, assumed to be related to contaminant burden, occurred in the late 1970s and early 1980s, but survival has since increased to equal survival in the

  12. Lake trout rehabilitation in Lake Huron

    USGS Publications Warehouse

    Eshenroder, Randy L.; Payne, N. Robert; Johnson, James E.; Bowen, Charles; Ebener, Mark P.

    1995-01-01

    Efforts to restore lake trout (Salvelinus namaycush) in Lake Huron after their collapse in the 1940s were underway in the early 1970s with completion of the first round of lampricide applications in tributary streams and the stocking of several genotypes. We assess results of rehabilitation and establish a historical basis for comparison by quantifying the catch of spawning lake trout from Michigan waters in 1929-1932. Sixty-eight percent of this catch occurred in northern waters (MH-1) and most of the rest (15%) was from remote reefs in the middle of the main basin. Sea lampreys (Petromyzon marinus) increased in the early 1980s in the main basin and depressed spawning populations of lake trout. This increase was especially severe in northern waters and appeared to be associated with untreated populations in the St. Marys River. Excessive commercial fishing stemming from unresolved treaty rights also contributed to loss of spawning fish in northern Michigan waters. Seneca-strain lake trout did not appear to be attacked by sea lampreys until they reached a size > 532 mm. At sizes > 632 mm, Seneca trout were 40-fold more abundant than the Marquette strain in matched-planting experiments. Natural reproduction past the fry stage has occurred in Thunder Bay and South Bay, but prospects for self-sustaining populations of lake trout in the main basin are poor because sea lampreys are too abundant, only one side of the basin is stocked, and stocking is deferred to allow commercial gillnetting in areas where most of the spawning occurred historically. Backcross lake trout, a lake trout x splake (s. Fontinalis x s. Namaycush) hybrid, did not reproduce in Georgian Bay, but this genotype is being replaced with pure-strain lake trout, whose early performance appears promising.

  13. Evolution of alkaline lakes - Lake Van case study

    NASA Astrophysics Data System (ADS)

    Tillman Meyer, Felix; Viehberg, Finn; Bahroun, Sonya; Wolf, Annabel; Immenhauser, Adrian; Kwiecien, Ola

    2017-04-01

    Lake Van in Eastern Anatolia (Turkey) is the largest terminal soda lake on Earth. The lake sedimentary profile covers ca. 600 ka (Stockhecke et al. 2014) Based on lithological changes, the presence of freshwater microfossils and close-to-freshwater pH value in the pore water, members of ICDP PALEOVAN concluded that Lake Van might have started as an open lake. Here we show paleontological and geochemical evidence in favour of this idea and constrain the time, when Lake Van likely transformed into a closed lake. Additionally we provide the first conceptual model of how this closure may have happened. Our archives of choice are inorganic and biogenic carbonates, separated by wet sieving. We identified microfossil assemblages (fraction > 125 µm) and performed high-resolution oxygen isotope (delta18O) and elemental (Mg/Ca, Sr/Ca) analyses of the fraction < 63 µm assuming that it represents only carbonates precipitating in the water column. Microfossil assemblage consists of three different species of ostracods (Candona spp, Loxoconcha sp, Amnicythere spp.), diatoms, gastropods and bivalves. Brakish-water ostracods, Loxoconcha sp and Amnicythere sp occur more often after 530 ka. Additionaly, Loxoconcha sp is a shallow-water species relaying on plants growing in the photic zone as food supply. These two aspects point to an increasing salinity in a shallowing lake. The delta18O values of inorganic carbonates are relatively low during the initial phase of Lake Van and increase abruptly (ca. 7‰) after 530 ka BP. At approximately the same time combination of Sr/Ca and Mg/Ca data suggest first occurrence of aragonite. Again, these findings suggest geochemical changes of the lake water concurrent with transition documented by microfossils. Comparison between Lake Van and Lake Ohrid (Lacey et al. 2016) delta18O data, precludes regional climate change (e.g.: increased evaporation) as the main driver of observed changes. With no evidence for increased volcanic or tectonic

  14. Embryotoxicity of an extract from Great Lakes lake trout to rainbow trout and lake trout

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wright, P.J.; Tillitt, D.E.

    1995-12-31

    Aquatic ecosystems such as the Great Lakes are known to be contaminated with chemicals that are toxic to fish. However, the role of these contaminants in reproductive failures of fishes, such as lake trout recruitment, has remained controvertible. It was the objective to evaluate dioxin-like embryotoxicity of a complex mixture of chemicals and predict their potential to cause the lack of recruitment in Great Lakes lake trout. Graded doses of a complex environmental extract were injected into eggs of both rainbow trout and lake trout. The extract was obtained from whole adult lake trout collected from Lake Michigan in 1988.more » The extract was embryotoxic in rainbow trout, with LD50 values for Arlee strain and Erwin strain of 33 eggEQ and 14 eggEQ respectively. The LOAEL for hemorrhaging, yolk-sac edema, and craniofacial deformities in rainbow trout were 2, 2, and 4 eggEQ, respectively. Subsequent injections of the extract into lake trout eggs were likewise embryotoxic, with an LD50 value of 7 eggEQ. The LOAEL values for the extract in lake trout for hemorrhaging, yolk-sac edema, and craniofacial deformities were 0.1, 1, and 2 eggEQ, respectively. The current levels of contaminants in lake trout eggs are above the threshold for hemorrhaging and yolk-sac edema. The results also support the use of an additive model of toxicity to quantify PCDDs, PCDFs, Non-o-PCBs, and Mono-o-PCBs in relation to early life stage mortality in Lake Michigan lake trout.« less

  15. Movement patterns of Bar-headed Geese Anser indicus during breeding and post-breeding periods at Qinghai Lake, China

    USGS Publications Warehouse

    Cui, Peng; Hou, Yuansheng; Tang, Mingjie; Zhang, Haiting; Zuohua, Yuanchun; Yin, Zuohua; Li, Tianxian; Guo, Shan; Xing, Zhi; He, Yubang; Prosser, Diann J.; Newman, Scott H.; Takekawa, John Y.; Yan, Baoping; Lei, Fumin

    2011-01-01

    The highly pathogenic avian influenza (HPAI) H5N1 outbreak at Qinghai Lake, China, in 2005 caused the death of over 6,000 migratory birds, half of which were Bar-headed Geese Anser indicus. Understanding the movements of this species may inform monitoring of outbreak risks for HPAI viruses; thus, we investigated the movement patterns of 29 Bar-headed Geese at Qinghai Lake, China during 2007 and 2008 by using high resolution GPS satellite telemetry. We described the movements and distribution of marked Bar-headed Geese during the pre-nesting, nesting, and moulting periods. Of 21 Bar-headed Geese with complete transmission records, 3 moved to other areas during the nesting period: 2 to Jianghe wetland (50 km northwest of Qinghai Lake) and 1 to Cuolongka Lake (220 km northwest of Qinghai Lake) during the nesting period. We identified nesting attempts of 7 of the marked geese at Qinghai Lake. Four completed successful nesting attempts according to our rules of judgment for the breeding status, and 2 geese lost broods soon after hatching (hereafter referred to as unsuccessful breeders). Of 18 geese present at Qinghai Lake during the nesting period, 9 (6 non-breeders, 2 successful breeders and 1 unsuccessful breeder) remained at Qinghai Lake during the moulting period; and 9 (5 non-breeders, 4 unsuccessful breeders) left Qinghai Lake for moulting. Kuhai Lake, Donggeicuona Lake, Alake Lake, Zhaling-Eling Lake area and Huangheyuan wetland area were used as moulting sites. Geese that moulted at Qinghai Lake, Cuolongka Lake, Kuhai Lake, Donggeicuona Lake and Alake Lake also moved to Zhaling-Eling Lake area or Huangheyuan wetland area and stayed there for several days prior to autumn migration. Mean home range and core area estimates did not differ significantly by sex, year and between breeders and non-breeders.

  16. Movement patterns of Bar-headed Geese Anser indicus during breeding and post-breeding periods at Qinghai Lake, China

    USGS Publications Warehouse

    Cui, P.; Hou, Y.; Tang, M.; Zhang, H.; Zhou, Y.; Yin, Z.; Li, T.; Guo, S.; Xing, Z.; He, Y.; Prosser, D.J.; Newman, S.H.; Takekawa, John Y.; Yan, B.; Lei, F.

    2011-01-01

    The highly pathogenic avian influenza (HPAI) H5N1 outbreak at Qinghai Lake, China, in 2005 caused the death of over 6,000 migratory birds, half of which were Bar-headed Geese Anser indicus. Understanding the movements of this species may inform monitoring of outbreak risks for HPAI viruses; thus, we investigated the movement patterns of 29 Bar-headed Geese at Qinghai Lake, China during 2007 and 2008 by using high resolution GPS satellite telemetry. We described the movements and distribution of marked Bar-headed Geese during the pre-nesting, nesting, and moulting periods. Of 21 Bar-headed Geese with complete transmission records, 3 moved to other areas during the nesting period: 2 to Jianghe wetland (50 km northwest of Qinghai Lake) and 1 to Cuolongka Lake (220 km northwest of Qinghai Lake) during the nesting period. We identified nesting attempts of 7 of the marked geese at Qinghai Lake. Four completed successful nesting attempts according to our rules of judgment for the breeding status, and 2 geese lost broods soon after hatching (hereafter referred to as unsuccessful breeders). Of 18 geese present at Qinghai Lake during the nesting period, 9 (6 non-breeders, 2 successful breeders and 1 unsuccessful breeder) remained at Qinghai Lake during the moulting period; and 9 (5 non-breeders, 4 unsuccessful breeders) left Qinghai Lake for moulting. Kuhai Lake, Donggeicuona Lake, Alake Lake, Zhaling-Eling Lake area and Huangheyuan wetland area were used as moulting sites. Geese that moulted at Qinghai Lake, Cuolongka Lake, Kuhai Lake, Donggeicuona Lake and Alake Lake also moved to Zhaling-Eling Lake area or Huangheyuan wetland area and stayed there for several days prior to autumn migration. Mean home range and core area estimates did not differ significantly by sex, year and between breeders and non-breeders. ?? 2010 Dt. Ornithologen-Gesellschaft e.V.

  17. Modelling the mid-Pliocene Warm Period with the IPSLGCM: contribution to PlioMIP and feedback mechanisms from the presence of mega-lakes

    NASA Astrophysics Data System (ADS)

    Contoux, C.; Jost, A.; Sepulchre, P.; Ramstein, G.

    2012-04-01

    The mid-Pliocene Warm Period (mPWP, ca. 3.3 -3 Ma) is the last geological period showing a warmer climate than the preindustrial during a sustained period of time, much longer than interglacial periods of the last million years. Moreover, mPWP position of the continents and atmospheric pCO2 are very close to present-day, both conditions making the mPWP a relevant analogue for future global warming. For these reasons, the mPWP has been the focus of Pliocene Modelling Intercomparison Project (PlioMIP), which associates data analysis and modelling. We use the IPSLCM5 Earth System model and its atmospheric component alone (LMDZ), to simulate the climate of the mPWP. Boundary conditions such as sea surface temperatures (SSTs), topography, ice sheet extent and vegetation are the ones used within the PlioMIP framework. On a global scale we show the impact of different boundary conditions with LMDZ, and of a global coupling on the simulated climate. Results from the Earth System model are also compared to SST reconstructions, particularly in the North Atlantic Ocean, where an important warming occurs, generally poorly reproduced by models. These results will then be part of the multi-model analysis for the Pliocene. The PlioMIP exercise is also about better understanding model/data mismatches. In the present-day desertic regions of Lake Chad (Africa) and Lake Eyre (Australia), vegetation data show the presence of tropical savanna at the expense of deserts during the mPWP. Vegetation models forced by mPWP climatic simulations fail to reproduce more humid vegetation in these locations. There might be a reason for this model/data discrepancy: geological data stand for the presence of mega-lakes in these two regions during the mPWP that are not accounted for in previous simulations. Such extended waterbodies could have important feedbacks on the hydrological cycle and regional climate. We use the LMDZ4 atmospheric model imbedding explicitly resolved lake surfaces to simulate

  18. Hydrogeologic Controls on Lake Level at Mountain Lake, Virginia

    NASA Astrophysics Data System (ADS)

    Roningen, J. M.; Burbey, T. J.

    2011-12-01

    Mountain Lake in Giles County, Virginia has a documented history of severe natural lake-level changes involving groundwater seepage that extend over the past 4200 years. Featured in the 1986 movie Dirty Dancing, the natural lake dried up completely in September 2008 and levels have not yet recovered. A hydrogeologic investigation was undertaken in an effort to determine the factors influencing lake level changes. A daily water balance, dipole-dipole electrical resistivity surveying, well logging and chemical sampling have shed light on: 1) the influence of a fault not previously discussed in literature regarding the lake, 2) the seasonal response to precipitation of a forested first-order drainage system in fractured rock, and 3) the possibility of flow pathways related to karst features. Geologic controls on lake level were investigated using several techniques. Geophysical surveys using dipole-dipole resistivity located possible subsurface flowpaths both to and from the lake. Well logs, lineament analysis, and joint sampling were used to assess structural controls on lake hydrology. Major ions were sampled at wells, springs, streams, and the lake to evaluate possible mixing of different sources of water in the lake. Groundwater levels were monitored for correlation to lake levels, rainfall events, and possible seismic effects. The hydrology of the lake was quantified with a water balance on a daily time step. Results from the water balance indicate steady net drainage and significant recharge when vegetation is dormant, particularly during rain-on-snow melt events. The resistivity survey reveals discrete areas that represent flow pathways from the lake, as well as flowpaths to springs upgradient of the lake located in the vicinity of the fault. The survey also suggests that some flowpaths may originate outside of the topographic watershed of the lake. Chemical evidence indicates karst may underlie the lakebed. Historical data suggest that artificial intervention

  19. Lake-level frequency analysis for Devils Lake, North Dakota

    USGS Publications Warehouse

    Wiche, Gregg J.; Vecchia, Aldo V.

    1996-01-01

    Two approaches were used to estimate future lake-level probabilities for Devils Lake. The first approach is based on an annual lake-volume model, and the second approach is based on a statistical water mass-balance model that generates seasonal lake volumes on the basis of seasonal precipitation, evaporation, and inflow. Autoregressive moving average models were used to model the annual mean lake volume and the difference between the annual maximum lake volume and the annual mean lake volume. Residuals from both models were determined to be uncorrelated with zero mean and constant variance. However, a nonlinear relation between the residuals of the two models was included in the final annual lakevolume model.Because of high autocorrelation in the annual lake levels of Devils Lake, the annual lake-volume model was verified using annual lake-level changes. The annual lake-volume model closely reproduced the statistics of the recorded lake-level changes for 1901-93 except for the skewness coefficient. However, the model output is less skewed than the data indicate because of some unrealistically large lake-level declines. The statistical water mass-balance model requires as inputs seasonal precipitation, evaporation, and inflow data for Devils Lake. Analysis of annual precipitation, evaporation, and inflow data for 1950-93 revealed no significant trends or long-range dependence so the input time series were assumed to be stationary and short-range dependent.Normality transformations were used to approximately maintain the marginal probability distributions; and a multivariate, periodic autoregressive model was used to reproduce the correlation structure. Each of the coefficients in the model is significantly different from zero at the 5-percent significance level. Coefficients relating spring inflow from one year to spring and fall inflows from the previous year had the largest effect on the lake-level frequency analysis.Inclusion of parameter uncertainty in the model

  20. Lake-level frequency analysis for Devils Lake, North Dakota

    USGS Publications Warehouse

    Wiche, Gregg J.; Vecchia, Aldo V.

    1995-01-01

    Two approaches were used to estimate future lake-level probabilities for Devils Lake. The first approach is based on an annual lake-volume model, and the second approach is based on a statistical water mass-balance model that generates seasonal lake volumes on the basis of seasonal precipitation, evaporation, and inflow.Autoregressive moving average models were used to model the annual mean lake volume and the difference between the annual maximum lake volume and the annual mean lake volume. Residuals from both models were determined to be uncorrelated with zero mean and constant variance. However, a nonlinear relation between the residuals of the two models was included in the final annual lake-volume model.Because of high autocorrelation in the annual lake levels of Devils Lake, the annual lakevolume model was verified using annual lake-level changes. The annual lake-volume model closely reproduced the statistics of the recorded lake-level changes for 1901-93 except for the skewness coefficient However, the model output is less skewed than the data indicate because of some unrealistically large lake-level declines.The statistical water mass-balance model requires as inputs seasonal precipitation, evaporation, and inflow data for Devils Lake. Analysis of annual precipitation, evaporation, and inflow data for 1950-93 revealed no significant trends or long-range dependence so the input time series were assumed to be stationary and short-range dependent.Normality transformations were used to approximately maintain the marginal probability distributions; and a multivariate, periodic autoregressive model was used to reproduce the correlation structure. Each of the coefficients in the model is significantly different from zero at the 5-percent significance level. Coefficients relating spring inflow from one year to spring and fall inflows from the previous year had the largest effect on the lake-level frequency analysis.Inclusion of parameter uncertainty in the model

  1. Lake trout in northern Lake Huron spawn on submerged drumlins

    USGS Publications Warehouse

    Riley, Stephen C.; Binder, Thomas; Wattrus, Nigel J.; Faust, Matthew D.; Janssen, John; Menzies, John; Marsden, J. Ellen; Ebener, Mark P.; Bronte, Charles R.; He, Ji X.; Tucker, Taaja R.; Hansen, Michael J.; Thompson, Henry T.; Muir, Andrew M.; Krueger, Charles C.

    2014-01-01

    Recent observations of spawning lake trout Salvelinus namaycush near Drummond Island in northern Lake Huron indicate that lake trout use drumlins, landforms created in subglacial environments by the action of ice sheets, as a primary spawning habitat. From these observations, we generated a hypothesis that may in part explain locations chosen by lake trout for spawning. Most salmonines spawn in streams where they rely on streamflows to sort and clean sediments to create good spawning habitat. Flows sufficient to sort larger sediment sizes are generally lacking in lakes, but some glacial bedforms contain large pockets of sorted sediments that can provide the interstitial spaces necessary for lake trout egg incubation, particularly if these bedforms are situated such that lake currents can penetrate these sediments. We hypothesize that sediment inclusions from glacial scavenging and sediment sorting that occurred during the creation of bedforms such as drumlins, end moraines, and eskers create suitable conditions for lake trout egg incubation, particularly where these bedforms interact with lake currents to remove fine sediments. Further, these bedforms may provide high-quality lake trout spawning habitat at many locations in the Great Lakes and may be especially important along the southern edge of the range of the species. A better understanding of the role of glacially-derived bedforms in the creation of lake trout spawning habitat may help develop powerful predictors of lake trout spawning locations, provide insight into the evolution of unique spawning behaviors by lake trout, and aid in lake trout restoration in the Great Lakes.

  2. Lake sturgeon population characteristics in Rainy Lake, Minnesota and Ontario

    USGS Publications Warehouse

    Adams, W.E.; Kallemeyn, L.W.; Willis, D.W.

    2006-01-01

    Rainy Lake contains a native population of lake sturgeon Acipenser fulvescens that has been largely unstudied. The aims of this study were to document the population characteristics of lake sturgeon in Rainy Lake and to relate environmental factors to year-class strength for this population. Gill-netting efforts throughout the study resulted in the capture of 322 lake sturgeon, including 50 recaptures. Lake sturgeon in Rainy Lake was relatively plump and fast growing compared with a 32-population summary. Population samples were dominated by lake sturgeon between 110 and 150 cm total length. Age–structure analysis of the samples indicated few younger (<10 years) lake sturgeon, but the smallest gill net mesh size used for sampling was 102 mm (bar measure) and would not retain small sturgeon. Few lake sturgeon older than age 50 years were captured, and maximum age of sampled fish was 59 years. Few correlations existed between lake sturgeon year-class indices and both annual and monthly climate variables, except that mean June air temperature was positively correlated with year-class strength. Analysis of Rainy Lake water elevation and resulting lake sturgeon year-class strength indices across years yielded consistent but weak negative correlations between late April and early June, when spawning of lake sturgeon occurs. The baseline data collected in this study should allow Rainy Lake biologists to establish more specific research questions in the future.

  3. West Nile Virus Transmission in Winter: The 2013 Great Salt Lake Bald Eagle and Eared Grebes Mortality Event

    PubMed Central

    Ip, Hon S.; Van Wettere, Arnaud J.; McFarlane, Leslie; Shearn-Bochsler, Valerie; Dickson, Sammie Lee; Baker, JoDee; Hatch, Gary; Cavender, Kimberly; Long, Renee; Bodenstein, Barbara

    2014-01-01

    West Nile Virus (WNV) infection has been reported in over 300 species of birds and mammals. Raptors such as eagles, hawks and falcons are remarkably susceptible, but reports of WNV infection in Bald Eagles (Haliaeetus leucocephalus) are rare and reports of WNV infection in grebes (Podicipediformes) even rarer. We report an unusually large wild bird mortality event involving between 15,000-20,000 Eared Grebes (Podiceps nigricollis) and over 40 Bald Eagles around the Great Salt Lake, Utah, in November-December 2013. Mortality in grebes was first reported in early November during a period when the area was unseasonably warm and the grebes were beginning to gather and stage prior to migration. Ten out of ten Eared Grebes collected during this period were WNV RT-PCR and/or isolation positive. This is the first report of WNV infection in Eared Grebes and the associated mortality event is matched in scale only by the combined outbreaks in American White Pelican (Pelecanus erythrorhynchos) colonies in the north central states in 2002-2003. We cannot be sure that all of the grebes were infected by mosquito transmission; some may have become infected through contact with WNV shed orally or cloacally from other infected grebes. Beginning in early December, Bald Eagles in the Great Salt Lake area were observed to display neurological signs such as body tremors, limb paralysis and lethargy. At least 43 Bald Eagles had died by the end of the month. Nine of nine Bald Eagles examined were infected with WNV. To the best of our knowledge, this is the largest single raptor mortality event since WNV became endemic in the USA. Because the majority of the eagles affected were found after onset of below-freezing temperatures, we suggest at least some of the Bald Eagles were infected with WNV via consumption of infected Eared Grebes or horizontal transmission at roost sites. PMID:24761310

  4. Emergence and evolution of H10 subtype influenza viruses in poultry in China.

    PubMed

    Ma, Chi; Lam, Tommy Tsan-Yuk; Chai, Yujuan; Wang, Jia; Fan, Xiaohui; Hong, Wenshan; Zhang, Yu; Li, Lifeng; Liu, Yongmei; Smith, David K; Webby, Richard J; Peiris, Joseph S M; Zhu, Huachen; Guan, Yi

    2015-04-01

    The cases of human infections with H10N8 viruses identified in late 2013 and early 2014 in Jiangxi, China, have raised concerns over the origin, prevalence, and development of these viruses in this region. Our long-term influenza surveillance of poultry and migratory birds in southern China in the past 12 years showed that H10 influenza viruses have been introduced from migratory to domestic ducks over several winter seasons at sentinel duck farms at Poyang Lake, where domestic ducks share their water body with overwintering migratory birds. H10 viruses were never detected in terrestrial poultry in our survey areas until August 2013, when they were identified at live-poultry markets in Jiangxi. Since then, we have isolated 124 H10N8 or H10N6 viruses from chickens at local markets, revealing an ongoing outbreak. Phylogenetic analysis of H10 and related viruses showed that the chicken H10N8 viruses were generated through multiple reassortments between H10 and N8 viruses from domestic ducks and the enzootic chicken H9N2 viruses. These chicken reassortant viruses were highly similar to the human isolate, indicating that market chickens were the source of human infection. Recently, the H10 viruses further reassorted, apparently with H5N6 viruses, and generated an H10N6 variant. The emergence and prevalence of H10 viruses in chickens and the occurrence of human infections provide direct evidence of the threat from the current influenza ecosystem in China. After the outbreak of avian-origin H7N9 influenza viruses in China, fatal human infections with a novel H10N8 virus were reported. Utilizing data from 12 years of influenza surveillance in southern China, we showed that H10 viruses were regularly introduced by migratory ducks to domestic ducks on Poyang Lake, a major aggregative site of migratory birds in Asia. The H10 viruses were maintained and amplified in domestic ducks and then transmitted to chickens and reassorted with enzootic H9N2 viruses, leading to an

  5. Lake Level Variation in Small Lakes: Not a Clear Picture

    NASA Astrophysics Data System (ADS)

    Starratt, S.

    2017-12-01

    Lake level is a useful tool for identifying regional changes in precipitation and evaporation. Due to the volume of water in large lakes, they may only record large-scale changes in water balance, while smaller lakes may record more subtle variations. However, the record of water level in small lakes is affected by a number of factors including elevation, bathymetry, nutrient load, and aquatic macrophyte abundance. The latest Quaternary diatom records from three small lakes with areas of <10 ha (Hobart Lake, OR, 1458 masl; Swamp Lake, CA, 1554 masl; Favre Lake, NV, 2899 masl) and a larger lake (Medicine Lake, CA, 2036 masl, 154 ha) were compared in this study. All the lakes have a deep central basin (>10 m) surrounded by a shallow (1-2 m) shelf. Changes in the abundance of diatoms representing different life habits (benthic, tychoplanktic, planktic) were used to identify lake level variation. Benthic taxa dominate the assemblage when only the central basin is occupied. As the shallow shelf is flooded, the abundance of tychoplanktic taxa increases. Planktic taxa increase with the establishment of stratification. Favre Lake presents the clearest indication of initial lake level rise (7600-5750 cal yr BP) and intermittent flooding of the shelf for the remainder of the record. Stratification appears to become established only in the last few hundred years. Higher nutrient levels in the early part of the Hobart Lake record lead to a nearly monotypic planktic assemblage which is replaced by a tychoplanktic-dominated assemblage as the lake floods the shelf at about 3500 cal yr BP. The last 500 years is dominated by benthic taxa associated with aquatic macrophytes. The consistent presence of planktic taxa in the Swamp Lake record suggests that the lake was stratified during most of its history, although slight variations in the relative abundances of planktic and tychoplanktic groups occur. The Medicine Lake record shows a gradual increase in planktic species between 11

  6. Provision of healthcare in a remote base of operations in southern Chad.

    PubMed

    Hickey, J P

    2010-09-01

    The Irish Defence Forces maintained a presence in south eastern Chad under the authority of the United Nations Security Council from January 2008 until May 2010, operating in a peace support role as the lead contingent in a multinational battalion. In September 2009 the task of establishing a forward operating base in a remote location within the area of operations was ordered by mission headquarters. Irish and Finnish troops duly deployed and began the task of establishing a safe and secure base from which to operate. This involved securing the location, installing accommodation, electricity, lighting and facilities for ablutions, removing natural hazards, establishing secure communications and ensuring rapid access and egress in the event of hostile contact or emergency. The incidence of disease at this location was low, with the notable exception of a limited outbreak of gastroenteritis. The high standard of engineering work carried out around the camp, especially the latrines, washing facilities and other hygiene measures, significantly contributed to minimising the transmission of infectious disease. The past experiences of the Defence Forces in Congo, Lebanon, East Timor, Eritrea and Liberia have led to a high standard of forward planning and logistical awareness within the organisation which served personnel well in this latest mission. Copyright © 2010 Elsevier Ltd. All rights reserved.

  7. Factors determining water treatment behavior for the prevention of cholera in Chad.

    PubMed

    Lilje, Jonathan; Kessely, Hamit; Mosler, Hans-Joachim

    2015-07-01

    Cholera is a well-known and feared disease in developing countries, and is linked to high rates of morbidity and mortality. Contaminated drinking water and the lack of sufficient treatment are two of the key causes of high transmission rates. This article presents a representative health survey performed in Chad to inform future intervention strategies in the prevention and control of cholera. To identify critical psychological factors for behavior change, structured household interviews were administered to N = 1,017 primary caregivers, assessing their thoughts and attitudes toward household water treatment according to the Risk, Attitude, Norm, Ability, and Self-regulation model. The intervention potential for each factor was estimated by analyzing differences in means between groups of current performers and nonperformers of water treatment. Personal risk evaluation for diarrheal diseases and particularly for cholera was very low among the study population. Likewise, the perception of social norms was found to be rather unfavorable for water treatment behaviors. In addition, self-reported ability estimates (self-efficacy) revealed some potential for intervention. A mass radio campaign is proposed, using information and normative behavior change techniques, in combination with community meetings focused on targeting abilities and personal commitment to water treatment. © The American Society of Tropical Medicine and Hygiene.

  8. 78 FR 53675 - Safety Zone; Lake Erie Heritage Foundation, Battle of Lake Erie Reenactment; Lake Erie, Put-in...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-30

    ...-AA00 Safety Zone; Lake Erie Heritage Foundation, Battle of Lake Erie Reenactment; Lake Erie, Put-in-Bay... temporary safety zone in the waters of Lake Erie in the vicinity of Put-In-Bay, OH. This safety zone is intended to restrict vessels from a portion of Lake Erie during Battle of Lake Erie Reenactment near Put-In...

  9. Monovalent virus-like particle vaccine protects guinea pigs and nonhuman primates against infection with multiple Marburg viruses.

    PubMed

    Swenson, Dana L; Warfield, Kelly L; Larsen, Tom; Alves, D Anthony; Coberley, Sadie S; Bavari, Sina

    2008-05-01

    Virus-like particle (VLP)-based vaccines have the advantage of being morphologically and antigenically similar to the live virus from which they are derived. Expression of the glycoprotein and VP40 matrix protein from Lake Victoria marburgvirus (MARV) results in spontaneous production of VLPs in mammalian cells. Guinea pigs vaccinated with Marburg virus VLPs (mVLPs) or inactivated MARV (iMARV) develop homologous humoral and T-cell responses and are completely protected from a lethal homologous MARV challenge. To determine whether mVLPs based on the Musoke (aka Lake Victoria) isolate of MARV could broadly protect against diverse isolates of MARV, guinea pigs were vaccinated with mVLPs or iMARV-Musoke and challenged with MARV-Musoke, -Ravn or -Ci67. Prior to challenge, the mVLP- and iMARV-vaccinated guinea pigs had high levels of homologous MARV-Musoke and heterologous MARV-Ravn and -Ci67 antibodies. The Musoke-based mVLPs and iMARV vaccines provided complete protection in guinea pigs against viremia, viral replication and pathological changes in tissues, and lethal disease following challenge with MARV-Musoke, -Ravn or -Ci67. Guinea pigs vaccinated with RIBI adjuvant alone and infected with guinea pig-adapted MARV-Musoke, -Ravn or -Ci67 had histopathologic findings similar to those seen in the nonhuman primate model for MARV infection. Based on the strong protection observed in guinea pigs, we next vaccinated cynomolgus macaques with Musoke-based mVLPs and showed the VLP-vaccinated monkeys were broadly protected against three isolates of MARV (Musoke, Ravn and Ci67). Musoke mVLPs are effective at inducing broad heterologous immunity and protection against multiple MARV isolates.

  10. Etiological, clinical, and therapeutic aspects of acute generalized peritonitis in N'Djamena, Chad.

    PubMed

    Choua, O; Ali, M M; Kaboro, M; Moussa, K M; Anour, M

    2017-08-01

    Our aim was to define the epidemiological profile of acute generalized peritonitis in N'Djamena, Chad. This retrospective study, conducted in the general surgery department of the National Reference General Hospital, examined the files of 492 patients who underwent surgery for acute generalized peritonitis from June 2007 to December 2012. Epidemiological, clinical, paraclinical, and therapeutic characteristics were described. Acute generalized peritonitis accounted for 35.2 % of all visceral surgical emergencies. Male patients were at highest risk (sex-ratio 6.5). The patients' mean age was 25.8 years (range 1 to 70 years). All patients had abdominal pain. The leading cause was traumatic visceral perforation by stabbing or a firearm in 226 cases (46 %), followed by diffuse appendiceal peritonitis. Primary peritonitis was rare. The principal procedure was surgical excision and suture. The mean time to consultation was 3 days and the mean hospital stay 8.5 days. The morbidity rate was 16.8 %, dominated by wound infection. The mortality rate was 6.8 %. Abdominal trauma is the major cause of acute generalized peritonitis in N'Djamena. Prognosis depends on time to surgical management.

  11. Prediction of new-onset atrial fibrillation after first-ever ischemic stroke: A comparison of CHADS2, CHA2DS2-VASc and HATCH scores and the added value of stroke severity.

    PubMed

    Hsieh, Cheng-Yang; Lee, Cheng-Han; Wu, Darren Philbert; Sung, Sheng-Feng

    2018-05-01

    Early detection of atrial fibrillation after stroke is important for secondary prevention in stroke patients without known atrial fibrillation (AF). We aimed to compare the performance of CHADS 2 , CHA 2 DS 2 -VASc and HATCH scores in predicting AF detected after stroke (AFDAS) and to test whether adding stroke severity to the risk scores improves predictive performance. Adult patients with first ischemic stroke event but without a prior history of AF were retrieved from a nationwide population-based database. We compared C-statistics of CHADS 2 , CHA 2 DS 2 -VASc and HATCH scores for predicting the occurrence of AFDAS during stroke admission (cohort I) and during follow-up after hospital discharge (cohort II). The added value of stroke severity to prediction models was evaluated using C-statistics, net reclassification improvement, and integrated discrimination improvement. Cohort I comprised 13,878 patients and cohort II comprised 12,567 patients. Among them, 806 (5.8%) and 657 (5.2%) were diagnosed with AF, respectively. The CHADS 2 score had the lowest C-statistics (0.558 in cohort I and 0.597 in cohort II), whereas the CHA 2 DS 2 -VASc score had comparable C-statistics (0.603 and 0.644) to the HATCH score (0.612 and 0.653) in predicting AFDAS. Adding stroke severity to each of the three risk scores significantly increased the model performance. In stroke patients without known AF, all three risk scores predicted AFDAS during admission and follow-up, but with suboptimal discrimination. Adding stroke severity improved their predictive abilities. These risk scores, when combined with stroke severity, may help prioritize patients for continuous cardiac monitoring in daily practice. Copyright © 2018 Elsevier B.V. All rights reserved.

  12. Lake trout spawning habitat in the Six Fathom Bank-Yankee Reef lake trout sanctuary, Lake Huron

    USGS Publications Warehouse

    Edsall, Thomas A.; Brown, Charles L.; Kennedy, Gregory W.; Poe, Thomas P.

    1992-01-01

    Attempts to reestablish self-sustaining stocks of lake trout (Salvelinus namaycush) in the lower four Great Lakes, where the species was extinguished in the 1950s and 1960s, have been largely unsuccessful. To avoid many of the problems believed to be contributing to this failure, the fishery management community recently established several sanctuaries in the offshore waters of the Great Lakes where the development and protection of self-sustaining stocks of lake trout would be a primary management objective. One of these, the Six Fathom Bank-Yankee Reef sanctuary, was created in the south-central portion of Lake Huron. This sanctuary covers 168,000 ha and includes the shallower portions of the Six Fathom and Ipperwash scarps, which are major bathymetric features in the southern half of the lake. Historical accounts describe Six Fathom Bank as the most important lake trout spawning ground in the lake. Here we present the results of lake bed surveys conducted in the sanctuary with side-scan sonar, underwater videocamera systems, and a small research submarine. Our observations of the lake bed are consistent with what is known of the bedrock stratigraphy, glacial history, and karst geomorphology of the Lake Huron basin. Most of the loose rock we found seemed to be derived from local carbonate bedrock formations, although non-carbonate rock probably from Precambrian sources to the north was also present in some areas. Much of the bedrock and loose rock displayed karst solution features described for the Bruce Peninsula on the Ontario shoreline. Our surveys revealed substantial areas of lake bed at water depths of 20–36 m that resembled suitable spawning and fry production habitat for the shallow-water strains of lake trout that are the focus of the rehabilitation effort. Low mid-lake nutrient levels documented recently by others and the extremely high abundance of Mysis relicta (an important item in the diet of young lake trout) that we documented on Yankee Reef

  13. Lake-level variability and water availability in the Great Lakes

    USGS Publications Warehouse

    Wilcox, Douglas A.; Thompson, Todd A.; Booth, Robert K.; Nicholas, J.R.

    2007-01-01

    In this report, we present recorded and reconstructed (pre-historical) changes in water levels in the Great Lakes, relate them to climate changes of the past, and highlight major water-availability implications for storage, coastal ecosystems, and human activities. 'Water availability,' as conceptualized herein, includes a recognition that water must be available for human and natural uses, but the balancing of how much should be set aside for which use is not discussed. The Great Lakes Basin covers a large area of North America. The lakes capture and store great volumes of water that are critical in maintaining human activities and natural ecosystems. Water enters the lakes mostly in the form of precipitation and streamflow. Although flow through the connecting channels is a primary output from the lakes, evaporation is also a major output. Water levels in the lakes vary naturally on timescales that range from hours to millennia; storage of water in the lakes changes at the seasonal to millennial scales in response to lake-level changes. Short-term changes result from storm surges and seiches and do not affect storage. Seasonal changes are driven by differences in net basin supply during the year related to snowmelt, precipitation, and evaporation. Annual to millennial changes are driven by subtle to major climatic changes affecting both precipitation (and resulting streamflow) and evaporation. Rebounding of the Earth's surface in response to loss of the weight of melted glaciers has differentially affected water levels. Rebound rates have not been uniform across the basin, causing the hydrologic outlet of each lake to rise in elevation more rapidly than some parts of the coastlines. The result is a long-term change in lake level with respect to shoreline features that differs from site to site. The reconstructed water-level history of Lake Michigan-Huron over the past 4,700 years shows three major high phases from 2,300 to 3,300, 1,100 to 2,000, and 0 to 800

  14. Lake Life.

    ERIC Educational Resources Information Center

    Ohrn, Deborah Gore, Ed.

    1993-01-01

    This quarterly publication of the State Historical Society of Iowa features articles and activities for elementary school students. This summer issue focuses on the topic of lake life. The issue includes the following features: (1) "Where the Lakes Are Map"; (2) "Letter from the Lake"; (3) "Lake People"; (4)…

  15. Increased piscivory by lake whitefish in Lake Huron

    USGS Publications Warehouse

    Pothoven, Steven A.; Madenjian, Charles P.

    2013-01-01

    We evaluated the diet of Lake Whitefish Coregonus clupeaformis in Lake Huron during 2002–2011 to determine the importance of Round Goby Neogobius melanostomus and other fish as prey items. Lake Whitefish that had reached approximately 400 mm in length incorporated fish into their diets. The overall percentage of adult Lake Whitefish in Lake Huron that had eaten fish increased from 10% in 2002–2006 to 20% in 2007–2011, with a corresponding decrease in the frequency of Lake Whitefish that ate Dreissena spp. from 52% to 33%. During 2002–2006, Round Goby (wet mass, 38%), sculpins (Cottidae) (34%), and Ninespine Stickleback Pungitius pungitius (18%) were the primary fish eaten, whereas Round Goby accounted for 92% of the fish eaten in 2007–2011. Overall, Round Goby were found in the fewest Lake Whitefish stomachs in the north region of Lake Huron (6%) and in the most in the central (23%) and south (19%) regions of the lake. In the central region, Round Goby were eaten during all seasons that were sampled (spring through fall). In the south region, Round Goby were eaten only in the winter and spring but not in the summer when Dreissena spp. and spiny water flea Bythotrephes longimanus dominated the diet. Based on the 2007–2011 diet composition, an individual Lake Whitefish would need to have increased their consumption relative to that in 1983–1994 by 6% in the north region, 12% in the central region, and 41% in the southern region in order to achieve the same growth that was observed before dreissenid mussels arrived. However, Lake Whitefish weight adjusted for length only increased by 2% between 2002–2006 and 2007–2011 in the central region, decreased by 4% in the northern region, and remained constant in the southern region. This suggests that a shift toward more frequent piscivory does not necessarily improve the condition of a generalist feeder like Lake Whitefish.

  16. Holocene lake-level fluctuations of Lake Aricota, Southern Peru

    USGS Publications Warehouse

    Placzek, C.; Quade, Jay; Betancourt, J.L.

    2001-01-01

    Lacustrine deposits exposed around Lake Aricota, Peru (17?? 22???S), a 7.5-km2 lake dammed by debris flows, provide a middle to late Holocene record of lake-level fluctuations. Chronological context for shoreline deposits was obtained from radiocarbon dating of vascular plant remains and other datable material with minimal 14C reservoir effects (<350 yr). Diatomites associated with highstands several meters above the modern lake level indicate wet episodes. Maximum Holocene lake level was attained before 6100 14C yr B.P. and ended ???2700 14C yr B.P. Moderately high lake levels occurred at 1700 and 1300 14C yr B.P. The highstand at Lake Aricota during the middle Holocene is coeval with a major lowstand at Lake Titicaca (16?? S), which is only 130 km to the northeast and shares a similar climatology. Comparisons with other marine and terrestrial records highlight emerging contradictions over the nature of mid-Holocene climate in the central Andes. ?? 2001 University of Washington.

  17. Holocene Lake-Level Fluctuations of Lake Aricota, Southern Peru

    NASA Astrophysics Data System (ADS)

    Placzek, Christa; Quade, Jay; Betancourt, Julio L.

    2001-09-01

    Lacustrine deposits exposed around Lake Aricota, Peru (17° 22‧S), a 7.5-km2 lake dammed by debris flows, provide a middle to late Holocene record of lake-level fluctuations. Chronological context for shoreline deposits was obtained from radiocarbon dating of vascular plant remains and other datable material with minimal 14C reservoir effects (<350 yr). Diatomites associated with highstands several meters above the modern lake level indicate wet episodes. Maximum Holocene lake level was attained before 6100 14C yr B.P. and ended ∼2700 14C yr B.P. Moderately high lake levels occurred at 1700 and 1300 14C yr B.P. The highstand at Lake Aricota during the middle Holocene is coeval with a major lowstand at Lake Titicaca (16°S), which is only 130 km to the northeast and shares a similar climatology. Comparisons with other marine and terrestrial records highlight emerging contradictions over the nature of mid-Holocene climate in the central Andes.

  18. Effects of the Boko Haram Insurgency Group in West and Central Africa

    DTIC Science & Technology

    2016-06-10

    Programme predicted people would eventually run out of 98 Fessy. 99 Yahoo News, “Boko Haram...000-pupils-niger-195800803.html?ref=gs. 100 Ibid. 101 Fessy. 102 Yahoo News, “Boko Haram attacks force 12,000 pupils from Niger schools: UN...Lake Chad.136 135 Yahoo News, “Cameroon Pledges 2,500 Troops for Regional Boko Haram Force,” 14

  19. Aspects of a Grammar of Makary Kotoko (Chadic, Cameroon)

    ERIC Educational Resources Information Center

    Allison, Sean David

    2012-01-01

    Makary Kotoko (MK), a Central Chadic B language, is spoken in the north of Cameroon just south of Lake Chad. Published works on MK to date include about a dozen articles on different aspects of the grammar of the language, primarily by H. Tourneux. The present work, which is based on a substantial corpus of recorded texts, is a systematic…

  20. Reevaluation of lake trout and lake whitefish bioenergetics models

    USGS Publications Warehouse

    Madenjian, Charles P.; Pothoven, Steve A.; Kao, Yu-Chun

    2013-01-01

    Using a corrected algorithm for balancing the energy budget, we reevaluated the Wisconsin bioenergetics model for lake trout (Salvelinus namaycush) in the laboratory and for lake whitefish (Coregonus clupeaformis) in the laboratory and in the field. For lake trout, results showed that the bioenergetics model slightly overestimated food consumption by the lake trout when they were fed low and intermediate rations, whereas the model predicted food consumption by lake trout fed ad libitum without any detectable bias. The slight bias in model predictions for lake trout on restricted rations may have been an artifact of the feeding schedule for these fish, and we would therefore recommend application of the Wisconsin lake trout bioenergetics model to lake trout populations in the field without any revisions to the model. Use of the Wisconsin bioenergetics model for coregonids resulted in overestimation of food consumption by lake whitefish both in the laboratory and in the field by between 20 and 30%, on average. This overestimation of food consumption was most likely due to overestimation of respiration rate. We therefore adjusted the respiration component of the bioenergetics model to obtain a good fit to the observed consumption in our laboratory tanks. The adjusted model predicted the consumption in the laboratory and the field without any detectable bias. Until a detailed lake whitefish respiration study can be conducted, we recommend application of our adjusted version of the Wisconsin generalized coregonid bioenergetics model to lake whitefish populations in the field.

  1. Continued Endemic Wild Poliovirus Transmission in Security-Compromised Areas - Nigeria, 2016.

    PubMed

    Nnadi, Chimeremma; Damisa, Eunice; Esapa, Lisa; Braka, Fiona; Waziri, Ndadilnasiya; Siddique, Anisur; Jorba, Jaume; Nganda, Gatei Wa; Ohuabunwo, Chima; Bolu, Omotayo; Wiesen, Eric; Adamu, Usman

    2017-02-24

    On August 10, 2016, 2 years after the most recent wild poliovirus (WPV) case was reported in Nigeria (in July 2014) (1), two WPV cases were reported in the northeastern state of Borno, which has been severely affected by insurgency-related insecurity since 2013. On September 9 and 26, 2016, two additional WPV cases were reported in Borno in children whose families migrated from security-compromised, inaccessible areas of the state. All four cases were WPV serotype 1 (WPV1), with genetic differences indicating prolonged undetected transmission. A large-scale emergency response plan was developed and implemented. The plan initially called for vaccination of 815,791 children during August 15-18 in five local government areas (LGAs) in the immediate vicinity of the first two WPV cases. Subsequently, the plan was expanded to regionally synchronized supplementary immunization activities (SIAs), conducted during August 27-December 6 in five Lake Chad basin countries at increased risk for national and regional WPV1 transmission (Cameroon, Central African Republic, Chad, Niger, and Nigeria). In addition, retrospective searches for missed cases of acute flaccid paralysis (AFP), enhanced environmental surveillance for polioviruses, and polio surveillance system reviews were conducted. Prolonged undetected WPV1 transmission in Borno State is a consequence of low population immunity and severe surveillance limitations associated with insurgency-related insecurity and highlights the risk for local and international WPV spread (2). Increasing polio vaccination coverage and implementing high-quality polio surveillance, especially among populations in newly secured and difficult-to-access areas in Borno and other Lake Chad basin areas are urgently needed.

  2. Induction and Maintenance of CX3CR1-Intermediate Peripheral Memory CD8+ T Cells by Persistent Viruses and Vaccines.

    PubMed

    Gordon, Claire Louse; Lee, Lian Ni; Swadling, Leo; Hutchings, Claire; Zinser, Madeleine; Highton, Andrew John; Capone, Stefania; Folgori, Antonella; Barnes, Eleanor; Klenerman, Paul

    2018-04-17

    The induction and maintenance of T cell memory is critical to the success of vaccines. A recently described subset of memory CD8 + T cells defined by intermediate expression of the chemokine receptor CX3CR1 was shown to have self-renewal, proliferative, and tissue-surveillance properties relevant to vaccine-induced memory. We tracked these cells when memory is sustained at high levels: memory inflation induced by cytomegalovirus (CMV) and adenovirus-vectored vaccines. In mice, both CMV and vaccine-induced inflationary T cells showed sustained high levels of CX3R1 int cells exhibiting an effector-memory phenotype, characteristic of inflationary pools, in early memory. In humans, CX3CR1 int CD8 + T cells were strongly induced following adenovirus-vectored vaccination for hepatitis C virus (HCV) (ChAd3-NSmut) and during natural CMV infection and were associated with a memory phenotype similar to that in mice. These data indicate that CX3CR1 int cells form an important component of the memory pool in response to persistent viruses and vaccines in both mice and humans. Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

  3. Late Holocene lake-level fluctuations in Walker Lake, Nevada, USA

    USGS Publications Warehouse

    Yuan, F.; Linsley, B.K.; Howe, S.S.; Lund, S.P.; McGeehin, J.P.

    2006-01-01

    Walker Lake, a hydrologically closed, saline, and alkaline lake, is situated along the western margin of the Great Basin in Nevada of the western United States. Analyses of the magnetic susceptibility (??), total inorganic carbon (TIC), and oxygen isotopic composition (??18O) of carbonate sediments including ostracode shells (Limnocythere ceriotuberosa) from Walker Lake allow us to extend the sediment record of lake-level fluctuations back to 2700??years B.P. There are approximately five major stages over the course of the late Holocene hydrologic evolution in Walker Lake: an early lowstand (> 2400??years B.P.), a lake-filling period (??? 2400 to ??? 1000??years B.P.), a lake-level lowering period during the Medieval Warm Period (MWP) (??? 1000 to ??? 600??years B.P.), a relatively wet period (??? 600 to ??? 100??years B.P.), and the anthropogenically induced lake-level lowering period (< 100??years B.P.). The most pronounced lowstand of Walker Lake occurred at ??? 2400??years B.P., as indicated by the relatively high values of ??18O. This is generally in agreement with the previous lower resolution paleoclimate results from Walker Lake, but contrasts with the sediment records from adjacent Pyramid Lake and Siesta Lake. The pronounced lowstand suggests that the Walker River that fills Walker Lake may have partially diverted into the Carson Sink through the Adrian paleochannel between 2700 to 1400??years B.P. ?? 2006 Elsevier B.V. All rights reserved.

  4. Bathythermal habitat use by strains of Great Lakes- and Finger Lakes-origin lake trout in Lake Huron after a change in prey fish abundance and composition

    USGS Publications Warehouse

    Bergstedt, Roger A.; Argyle, Ray L.; Krueger, Charles C.; Taylor, William W.

    2012-01-01

    A study conducted in Lake Huron during October 1998–June 2001 found that strains of Great Lakes-origin (GLO) lake trout Salvelinus namaycush occupied significantly higher temperatures than did Finger Lakes-origin (FLO; New York) lake trout based on data from archival (or data storage) telemetry tags that recorded only temperature. During 2002 and 2003, we implanted archival tags that recorded depth as well as temperature in GLO and FLO lake trout in Lake Huron. Data subsequently recorded by those tags spanned 2002–2005. Based on those data, we examined whether temperatures and depths occupied by GLO and FLO lake trout differed during 2002–2005. Temperatures occupied during those years were also compared with occupied temperatures reported for 1998–2001, before a substantial decline in prey fish biomass. Temperatures occupied by GLO lake trout were again significantly higher than those occupied by FLO lake trout. This result supports the conclusion of the previous study. The GLO lake trout also occupied significantly shallower depths than FLO lake trout. In 2002–2005, both GLO and FLO lake trout occupied significantly lower temperatures than they did in 1998–2001. Aside from the sharp decline in prey fish biomass between study periods, the formerly abundant pelagic alewife Alosa pseudoharengus virtually disappeared and the demersal round goby Neogobius melanostomus invaded the lake and became locally abundant. The lower temperatures occupied by lake trout in Lake Huron during 2002–2005 may be attributable to changes in the composition of the prey fish community, food scarcity (i.e., a retreat to cooler water could increase conversion efficiency), or both.

  5. Chemours Pompton Lakes Works Site, Pompton Lakes, NJ

    EPA Pesticide Factsheets

    E.I. DuPont De Nemours & Company is located at 2000 Cannonball Road, Pompton Lakes, New Jersey. The DuPont Pompton Lakes Works site (DuPont) occupies approximately 570 acres of land in Pompton Lakes and Wanaque.

  6. Porewater salinity reveals past lake-level changes in Lake Van, the Earth's largest soda lake.

    PubMed

    Tomonaga, Yama; Brennwald, Matthias S; Livingstone, David M; Kwiecien, Olga; Randlett, Marie-Ève; Stockhecke, Mona; Unwin, Katie; Anselmetti, Flavio S; Beer, Jürg; Haug, Gerald H; Schubert, Carsten J; Sturm, Mike; Kipfer, Rolf

    2017-03-22

    In closed-basin lakes, sediment porewater salinity can potentially be used as a conservative tracer to reconstruct past fluctuations in lake level. However, until now, porewater salinity profiles did not allow quantitative estimates of past lake-level changes because, in contrast to the oceans, significant salinity changes (e.g., local concentration minima and maxima) had never been observed in lacustrine sediments. Here we show that the salinity measured in the sediment pore water of Lake Van (Turkey) allows straightforward reconstruction of two major transgressions and a major regression that occurred during the last 250 ka. We observed strong changes in the vertical salinity profiles of the pore water of the uppermost 100 m of the sediments in Lake Van. As the salinity balance of Lake Van is almost at steady-state, these salinity changes indicate major lake-level changes in the past. In line with previous studies on lake terraces and with seismic and sedimentological surveys, we identify two major transgressions of up to +105 m with respect to the current lake level at about 135 ka BP and 248 ka BP starting at the onset of the two previous interglacials (MIS5e and MIS7), and a major regression of about -200 m at about 30 ka BP during the last ice age.

  7. ChAd63-MVA-vectored blood-stage malaria vaccines targeting MSP1 and AMA1: assessment of efficacy against mosquito bite challenge in humans.

    PubMed

    Sheehy, Susanne H; Duncan, Christopher J A; Elias, Sean C; Choudhary, Prateek; Biswas, Sumi; Halstead, Fenella D; Collins, Katharine A; Edwards, Nick J; Douglas, Alexander D; Anagnostou, Nicholas A; Ewer, Katie J; Havelock, Tom; Mahungu, Tabitha; Bliss, Carly M; Miura, Kazutoyo; Poulton, Ian D; Lillie, Patrick J; Antrobus, Richard D; Berrie, Eleanor; Moyle, Sarah; Gantlett, Katherine; Colloca, Stefano; Cortese, Riccardo; Long, Carole A; Sinden, Robert E; Gilbert, Sarah C; Lawrie, Alison M; Doherty, Tom; Faust, Saul N; Nicosia, Alfredo; Hill, Adrian V S; Draper, Simon J

    2012-12-01

    The induction of cellular immunity, in conjunction with antibodies, may be essential for vaccines to protect against blood-stage infection with the human malaria parasite Plasmodium falciparum. We have shown that prime-boost delivery of P. falciparum blood-stage antigens by chimpanzee adenovirus 63 (ChAd63) followed by the attenuated orthopoxvirus MVA is safe and immunogenic in healthy adults. Here, we report on vaccine efficacy against controlled human malaria infection delivered by mosquito bites. The blood-stage malaria vaccines were administered alone, or together (MSP1+AMA1), or with a pre-erythrocytic malaria vaccine candidate (MSP1+ME-TRAP). In this first human use of coadministered ChAd63-MVA regimes, we demonstrate immune interference whereby responses against merozoite surface protein 1 (MSP1) are dominant over apical membrane antigen 1 (AMA1) and ME-TRAP. We also show that induction of strong cellular immunity against MSP1 and AMA1 is safe, but does not impact on parasite growth rates in the blood. In a subset of vaccinated volunteers, a delay in time to diagnosis was observed and sterilizing protection was observed in one volunteer coimmunized with MSP1+AMA1-results consistent with vaccine-induced pre-erythrocytic, rather than blood-stage, immunity. These data call into question the utility of T cell-inducing blood-stage malaria vaccines and suggest that the focus should remain on high-titer antibody induction against susceptible antigen targets.

  8. ChAd63-MVA–vectored Blood-stage Malaria Vaccines Targeting MSP1 and AMA1: Assessment of Efficacy Against Mosquito Bite Challenge in Humans

    PubMed Central

    Sheehy, Susanne H; Duncan, Christopher JA; Elias, Sean C; Choudhary, Prateek; Biswas, Sumi; Halstead, Fenella D; Collins, Katharine A; Edwards, Nick J; Douglas, Alexander D; Anagnostou, Nicholas A; Ewer, Katie J; Havelock, Tom; Mahungu, Tabitha; Bliss, Carly M; Miura, Kazutoyo; Poulton, Ian D; Lillie, Patrick J; Antrobus, Richard D; Berrie, Eleanor; Moyle, Sarah; Gantlett, Katherine; Colloca, Stefano; Cortese, Riccardo; Long, Carole A; Sinden, Robert E; Gilbert, Sarah C; Lawrie, Alison M; Doherty, Tom; Faust, Saul N; Nicosia, Alfredo; Hill, Adrian VS; Draper, Simon J

    2012-01-01

    The induction of cellular immunity, in conjunction with antibodies, may be essential for vaccines to protect against blood-stage infection with the human malaria parasite Plasmodium falciparum. We have shown that prime-boost delivery of P. falciparum blood-stage antigens by chimpanzee adenovirus 63 (ChAd63) followed by the attenuated orthopoxvirus MVA is safe and immunogenic in healthy adults. Here, we report on vaccine efficacy against controlled human malaria infection delivered by mosquito bites. The blood-stage malaria vaccines were administered alone, or together (MSP1+AMA1), or with a pre-erythrocytic malaria vaccine candidate (MSP1+ME-TRAP). In this first human use of coadministered ChAd63-MVA regimes, we demonstrate immune interference whereby responses against merozoite surface protein 1 (MSP1) are dominant over apical membrane antigen 1 (AMA1) and ME-TRAP. We also show that induction of strong cellular immunity against MSP1 and AMA1 is safe, but does not impact on parasite growth rates in the blood. In a subset of vaccinated volunteers, a delay in time to diagnosis was observed and sterilizing protection was observed in one volunteer coimmunized with MSP1+AMA1—results consistent with vaccine-induced pre-erythrocytic, rather than blood-stage, immunity. These data call into question the utility of T cell-inducing blood-stage malaria vaccines and suggest that the focus should remain on high-titer antibody induction against susceptible antigen targets. PMID:23089736

  9. Spatial and temporal genetic diversity of lake whitefish (Coregonus clupeaformis (Mitchill)) from Lake Huron and Lake Erie

    USGS Publications Warehouse

    Stott, Wendylee; Ebener, Mark P.; Mohr, Lloyd; Hartman, Travis; Johnson, Jim; Roseman, Edward F.

    2013-01-01

    Lake whitefish (Coregonus clupeaformis (Mitchill)) are important commercially, culturally, and ecologically in the Laurentian Great Lakes. Stocks of lake whitefish in the Great Lakes have recovered from low levels of abundance in the 1960s. Reductions in abundance, loss of habitat and environmental degradation can be accompanied by losses of genetic diversity and overall fitness that may persist even as populations recover demographically. Therefore, it is important to be able to identify stocks that have reduced levels of genetic diversity. In this study, we investigated patterns of genetic diversity at microsatellite DNA loci in lake whitefish collected between 1927 and 1929 (historical period) and between 1997 and 2005 (contemporary period) from Lake Huron and Lake Erie. Genetic analysis of lake whitefish from Lakes Huron and Erie shows that the amount of population structuring varies from lake to lake. Greater genetic divergences among collections from Lake Huron may be the result of sampling scale, migration patterns and demographic processes. Fluctuations in abundance of lake whitefish populations may have resulted in periods of increased genetic drift that have resulted in changes in allele frequencies over time, but periodic genetic drift was not severe enough to result in a significant loss of genetic diversity. Migration among stocks may have decreased levels of genetic differentiation while not completely obscuring stock boundaries. Recent changes in spatial boundaries to stocks, the number of stocks and life history characteristics of stocks further demonstrate the potential of coregonids for a swift and varied response to environmental change and emphasise the importance of incorporating both spatial and temporal considerations into management plans to ensure that diversity is preserved.

  10. Movements of hatchery-reared lake trout in Lake Superior

    USGS Publications Warehouse

    Pycha, Richard L.; Dryer, William R.; King, George R.

    1965-01-01

    The history of stocking of lake trout (Salvelinus namaycush) in the Great Lakes is reviewed. The study of movements is based on capture of 24,275 fin-clipped lake trout taken in experimental gill nets and trawls and commercial gill nets. Yearling lake trout planted from shore dispersed to 15-fath (27-m) depths in 3A? hr. Most fish remained within 2 miles (3.2 km) of the planting site 2 months, but within 4 months some fish had moved as much as 17 miles (27 km). The highest abundance of planted lake trout was in areas 2-4 miles (3.2-6.4 km) from the planting site even 3 years after release. Distance moved and size of fish were not correlated. Dispersal of lake trout begins at planting and probably continues until the fish are mature. Most movement was eastward in southern Lake Superior and followed the counterclockwise surface currents. Movement is most rapid in areas of strong currents and slowest in areas of weak currents or eddies. Movement to areas west of the Keweenaw Peninsula was insignificant from plantings in Keweenaw Bay and nil from other plantings farther east. Lake trout planted in the eastern third of the lake dispersed more randomly than those planted farther west. Few fish moved farther offshore than the 50-fath (91-m) contour. Lake trout planted in Canadian waters made insignificant contributions to populations in US waters.

  11. Streptomyces gamaensis sp. nov., a novel actinomycete with antifungal activity isolated from soil in Gama, Chad.

    PubMed

    Zhao, Shanshan; Ye, Lan; Liu, Chongxi; Abagana, Adam Yacoub; Zheng, Weiwei; Sun, Pengyu; Li, Jiansong; Xiang, Wensheng; Wang, Xiangjing

    2017-04-01

    During an investigation exploring potential sources of novel species and natural products, a novel actinomycete with antifungal activity, designated strain NEAU-Gz11 T , was isolated from a soil sample, which was collected from Gama, Chad. The isolate was found to have morphological and chemotaxonomic characteristics typical of members of the genus Streptomyces. 16S rRNA gene sequence similarity studies showed that strain NEAU-Gz11 T belongs to the genus Streptomyces with high sequence similarity to Streptomyces hiroshimensis JCM 4098 T (98.0 %). Similarities to other type strains of the genus Streptomyces were lower than 98.0 %. However, the physiological and biochemical characteristics and low levels of DNA-DNA relatedness could differentiate the isolate genotypically and phenotypically from S. hiroshimensis JCM 4098 T . Therefore, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces gamaensis sp. nov. is proposed. The type strain is NEAU-Gz11 T (=CGMCC 4.7304 T =DSM 101531 T ).

  12. Energy density of lake whitefish Coregonus clupeaformis in Lakes Huron and Michigan

    USGS Publications Warehouse

    Pothoven, S.A.; Nalepa, T.F.; Madenjian, C.P.; Rediske, R.R.; Schneeberger, P.J.; He, J.X.

    2006-01-01

    We collected lake whitefish Coregonus clupeaformis off Alpena and Tawas City, Michigan, USA in Lake Huron and off Muskegon, Michigan USA in Lake Michigan during 2002–2004. We determined energy density and percent dry weight for lake whitefish from both lakes and lipid content for Lake Michigan fish. Energy density increased with increasing fish weight up to 800 g, and then remained relatively constant with further increases in fish weight. Energy density, adjusted for weight, was lower in Lake Huron than in Lake Michigan for both small (≤800 g) and large fish (>800 g). Energy density did not differ seasonally for small or large lake whitefish or between adult male and female fish. Energy density was strongly correlated with percent dry weight and percent lipid content. Based on data from commercially caught lake whitefish, body condition was lower in Lake Huron than Lake Michigan during 1981–2003, indicating that the dissimilarity in body condition between the lakes could be long standing. Energy density and lipid content in 2002–2004 in Lake Michigan were lower than data for comparable sized fish collected in 1969–1971. Differences in energy density between lakes were attributed to variation in diet and prey energy content as well as factors that affect feeding rates such as lake whitefish density and prey abundance.

  13. Effects of lake trout refuges on lake whitefish and cisco in the Apostle Islands Region of Lake Superior

    USGS Publications Warehouse

    Zuccarino-Crowe , Chiara M.; Taylor, William W.; Hansen, Michael J.; Seider, Michael J.; Krueger, Charles C.

    2016-01-01

    Lake trout refuges in the Apostle Islands region of Lake Superior are analogous to the concept of marine protected areas. These refuges, established specifically for lake trout (Salvelinus namaycush) and closed to most forms of recreational and commercial fishing, were implicated as one of several management actions leading to successful rehabilitation of Lake Superior lake trout. To investigate the potential significance of Gull Island Shoal and Devils Island Shoal refuges for populations of not only lake trout but also other fish species, relative abundances of lake trout, lake whitefish (Coregonus clupeaformis), and cisco (Coregonus artedi) were compared between areas sampled inside versus outside of refuge boundaries. During 1982–2010, lake trout relative abundance was higher and increased faster inside the refuges, where lake trout fishing was prohibited, than outside the refuges. Over the same period, lake whitefish relative abundance increased faster inside than outside the refuges. Both evaluations provided clear evidence that refuges protected these species. In contrast, trends in relative abundance of cisco, a prey item of lake trout, did not differ significantly between areas inside and outside the refuges. This result did not suggest indirect or cascading refuge effects due to changes in predator levels. Overall, this study highlights the potential of species-specific refuges to benefit other fish species beyond those that were the refuges' original target. Improved understanding of refuge effects on multiple species of Great Lakes fishes can be valuable for developing rationales for refuge establishment and predicting associated fish community-level effects.

  14. Future volcanic lake research: revealing secrets from poorly studied lakes

    NASA Astrophysics Data System (ADS)

    Rouwet, D.; Tassi, F.; Mora-Amador, R. A.

    2012-04-01

    Volcanic lake research boosted after the 1986 Lake Nyos lethal gas burst, a limnic rather than volcanic event. This led to the formation of the IAVCEI-Commission on Volcanic Lakes, which grew out into a multi-disciplinary scientific community since the 1990's. At Lake Nyos, a degassing pipe is functional since 2001, and two additional pipes were added in 2011, aimed to prevent further limnic eruption events. There are between 150 and 200 volcanic lakes on Earth. Some acidic crater lakes topping active magmatic-hydrothermal systems are monitored continuously or discontinuously. Such detailed studies have shown their usefulness in volcanic surveillance (e.g. Ruapehu, Yugama-Kusatsu-Shiran, Poás). Others are "Nyos-type" lakes, with possible gas accumulation in bottom waters and thus potentially hazardous. "Nyos-type" lakes tend to remain stably stratified in tropical and sub-tropical climates (meromictic), leading to long-term gas build-up and thus higher potential risk. In temperate climates, such lakes tend to turn over in winter (monomictic), and thus liberating its gas charge yearly. We line out research strategies for the different types of lakes. We believe a complementary, multi-disciplinary approach (geochemistry, geophysics, limnology, biology, statistics, etc.) will lead to new insights and ideas, which can be the base for future following-up and monitoring. After 25 years of pioneering studies on rather few lakes, the scientific community should be challenged to study the many poorly studied volcanic lakes, in order to better constrain the related hazard, based on probabilistic approaches.

  15. Gas exchange on Mono Lake and Crowley Lake, California

    NASA Technical Reports Server (NTRS)

    Wanninkhof, Rik; Ledwell, James R.; Broecker, Wallace S.

    1987-01-01

    Gas exchange coefficients (k) have been determined for freshwater Crowley Lake and saline Mono Lake through the use of a man-made purposefully injected gas, SF6. The concentration decreased from an initial value of 40 to 4 pmol/L for Mono Lake and from 20 to 1 pmol/L for Crowley lake over a period of 6 wks. Wind-speed (u) records from anemometers on the shore of each lake made it possible to determine the relationship between k and u. The average u and k values for the experiment were identical for the two lakes, despite the large chemical differences. It is estimated that, for the u values observed over Mono Lake from July to December 1984, the exchange of CO2 occurred 2.5 times faster than without chemical enhancement. This is a factor of 4 lower than needed to explain the high invasion rate of C-14 produced by nuclear bomb tests.

  16. Conflict for Resources: Water in the Lake Chad Basin

    DTIC Science & Technology

    2009-01-01

    Security 19 (1994), http://www.library.utoronto.ca/pcs/evidence (accessed 10 February 2009). 11 Miriam Lowi, Water and Power (Cambridge, UK : Cambridge...states wage wars and how scarce natural resources influence the states. Carl von Clausewitz’s treatise, On War serves as a starting point for any...has lots of water, very little of it is freshwater. Miriam Lowi, author of Water and Power describes political dynamics of high and low politics. High

  17. Lake

    ERIC Educational Resources Information Center

    Wien, Carol Anne

    2008-01-01

    The lake is blue black and deep. It is a glaciated finger lake, clawed out of rock when ice retracted across Nova Scotia in a northerly direction during the last ice age. The lake is narrow, a little over a mile long, and deep, 90 to 190 feet in places according to local lore, off the charts in others. The author loves to swim there, with a sense…

  18. Reconnaissance data on lakes in the Alpine Lakes Wilderness Area, Washington

    USGS Publications Warehouse

    Dethier, David P.; Heller, Paul L.; Safioles, Sally A.

    1979-01-01

    Sixty lakes in the Alpine Lakes Wilderness Area have been sampled from rubber rafts or helicopter to obtain information on their physical setting and on present water-quality conditions. The lakes are located near the crest of the Cascade Range in Chelan and King Counties, Washington. Basic data from these lakes will be useful for planners concerned with lake and wilderness management, and of interest to hikers and other recreationists who use the lakes.

  19. 75 FR 20920 - Safety Zone; Lake Havasu Grand Prix, Lake Havasu, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-22

    ...-AA00 Safety Zone; Lake Havasu Grand Prix, Lake Havasu, AZ AGENCY: Coast Guard, DHS. ACTION: Temporary... of Lake Havasu on the Colorado River in Lake Havasu City, Arizona for the Lake Havasu Grand Prix... established in support of the Lake Havasu Grand Prix, a marine event that includes participating vessels...

  20. Water-Balance Model to Simulate Historical Lake Levels for Lake Merced, California

    NASA Astrophysics Data System (ADS)

    Maley, M. P.; Onsoy, S.; Debroux, J.; Eagon, B.

    2009-12-01

    Lake Merced is a freshwater lake located in southwestern San Francisco, California. In the late 1980s and early 1990s, an extended, severe drought impacted the area that resulted in significant declines in Lake Merced lake levels that raised concerns about the long-term health of the lake. In response to these concerns, the Lake Merced Water Level Restoration Project was developed to evaluate an engineered solution to increase and maintain Lake Merced lake levels. The Lake Merced Lake-Level Model was developed to support the conceptual engineering design to restore lake levels. It is a spreadsheet-based water-balance model that performs monthly water-balance calculations based on the hydrological conceptual model. The model independently calculates each water-balance component based on available climate and hydrological data. The model objective was to develop a practical, rule-based approach for the water balance and to calibrate the model results to measured lake levels. The advantage of a rule-based approach is that once the rules are defined, they enhance the ability to then adapt the model for use in future-case simulations. The model was calibrated to historical lake levels over a 70-year period from 1939 to 2009. Calibrating the model over this long historical range tested the model over a variety of hydrological conditions including wet, normal and dry precipitation years, flood events, and periods of high and low lake levels. The historical lake level range was over 16 feet. The model calibration of historical to simulated lake levels had a residual mean of 0.02 feet and an absolute residual mean of 0.42 feet. More importantly, the model demonstrated the ability to simulate both long-term and short-term trends with a strong correlation of the magnitude for both annual and seasonal fluctuations in lake levels. The calibration results demonstrate an improved conceptual understanding of the key hydrological factors that control lake levels, reduce uncertainty

  1. Temporal analysis of the honey bee microbiome reveals four novel viruses and seasonal prevalence of known viruses, Nosema, and Crithidia.

    PubMed

    Runckel, Charles; Flenniken, Michelle L; Engel, Juan C; Ruby, J Graham; Ganem, Donald; Andino, Raul; DeRisi, Joseph L

    2011-01-01

    Honey bees (Apis mellifera) play a critical role in global food production as pollinators of numerous crops. Recently, honey bee populations in the United States, Canada, and Europe have suffered an unexplained increase in annual losses due to a phenomenon known as Colony Collapse Disorder (CCD). Epidemiological analysis of CCD is confounded by a relative dearth of bee pathogen field studies. To identify what constitutes an abnormal pathophysiological condition in a honey bee colony, it is critical to have characterized the spectrum of exogenous infectious agents in healthy hives over time. We conducted a prospective study of a large scale migratory bee keeping operation using high-frequency sampling paired with comprehensive molecular detection methods, including a custom microarray, qPCR, and ultra deep sequencing. We established seasonal incidence and abundance of known viruses, Nosema sp., Crithidia mellificae, and bacteria. Ultra deep sequence analysis further identified four novel RNA viruses, two of which were the most abundant observed components of the honey bee microbiome (∼10(11) viruses per honey bee). Our results demonstrate episodic viral incidence and distinct pathogen patterns between summer and winter time-points. Peak infection of common honey bee viruses and Nosema occurred in the summer, whereas levels of the trypanosomatid Crithidia mellificae and Lake Sinai virus 2, a novel virus, peaked in January.

  2. Temporal Analysis of the Honey Bee Microbiome Reveals Four Novel Viruses and Seasonal Prevalence of Known Viruses, Nosema, and Crithidia

    PubMed Central

    Engel, Juan C.; Ruby, J. Graham; Ganem, Donald; Andino, Raul; DeRisi, Joseph L.

    2011-01-01

    Honey bees (Apis mellifera) play a critical role in global food production as pollinators of numerous crops. Recently, honey bee populations in the United States, Canada, and Europe have suffered an unexplained increase in annual losses due to a phenomenon known as Colony Collapse Disorder (CCD). Epidemiological analysis of CCD is confounded by a relative dearth of bee pathogen field studies. To identify what constitutes an abnormal pathophysiological condition in a honey bee colony, it is critical to have characterized the spectrum of exogenous infectious agents in healthy hives over time. We conducted a prospective study of a large scale migratory bee keeping operation using high-frequency sampling paired with comprehensive molecular detection methods, including a custom microarray, qPCR, and ultra deep sequencing. We established seasonal incidence and abundance of known viruses, Nosema sp., Crithidia mellificae, and bacteria. Ultra deep sequence analysis further identified four novel RNA viruses, two of which were the most abundant observed components of the honey bee microbiome (∼1011 viruses per honey bee). Our results demonstrate episodic viral incidence and distinct pathogen patterns between summer and winter time-points. Peak infection of common honey bee viruses and Nosema occurred in the summer, whereas levels of the trypanosomatid Crithidia mellificae and Lake Sinai virus 2, a novel virus, peaked in January. PMID:21687739

  3. White Lake AOC

    EPA Pesticide Factsheets

    White Lake is in Muskegon County along the eastern shore of Lake Michigan. It was named an Area of Concern on the Great Lakes under the Great Lakes Water Quality Agreement of 1987 and delisted in 2014.

  4. In-lake Modeling Recommendation Report for Lake Champlain TMDL

    EPA Pesticide Factsheets

    This report describes the recommended modeling approach for the in-lake modeling component of the Lake Champlain TMDL project. The report was prepared by Tetra Tech, with input from the Lake Champlain modeling workgroup. (TetraTech, 2012b)

  5. Adherence to treatment guidelines: the association between stroke risk stratified comparing CHADS2 and CHA2DS2-VASc score levels and warfarin prescription for adult patients with atrial fibrillation.

    PubMed

    Chapman, Scott A; St Hill, Catherine A; Little, Meg M; Swanoski, Michael T; Scheiner, Shellina R; Ware, Kenric B; Lutfiyya, M Nawal

    2017-02-11

    Ischemic stroke is a risk associated with atrial fibrillation (AF) and is estimated to occur five times more often in afflicted patients than in those without AF. Anti-thrombotic therapy is recommended for the prevention of ischemic stroke. Risk stratification tools, such as the CHADS 2 , and more recently the CHA 2 DS 2 -VASc, for predicting stroke in patients with AF have been developed to determine the level of stroke risk and assist clinicians in the selection of antithrombotic therapy. Warfarin, for stroke prevention in AF, is the most commonly prescribed anticoagulant in North America. The purpose of this study was to examine the utility of using the CHADS 2 score levels (low and high) in contrast to the CHA 2 DS 2 -VASc when examining the outcome of warfarin prescriptions for adult patients with AF. The CHA 2 DS 2 -VASc tool was not widely used in 2010, when the data analyzed were collected. It has only been since 2014 that CHA 2 DS 2 -VASc criteria has been recommended to guide anticoagulant treatment in updated AF treatment guidelines. Bivariate and multivariate data analysis strategies were used to analyze 2010 National Ambulatory Care Survey (NAMCS) data. NAMCS is designed to collect data on the use and provision of ambulatory care services nationwide. The study population for this research was US adults with a diagnosis of AF. Warfarin prescription was the dependent variable for this study. The study population was 7,669,844 AF patients. Bivariate analysis revealed that of those AF patients with a high CHADS 2 score, 25.1% had received a warfarin prescription and 18.8 for those with a high CHA 2 DS 2 -VASc score. Logistic regression analysis yielded that patients with AF had higher odds of having a warfarin prescription if they had a high CHADS 2 score, were Caucasian, lived in a zip code where < 20% of the population had a university education, and lived in a zip code where < 10% of the population were living in households with incomes below

  6. Water quality of Lake Austin and Town Lake, Austin, Texas

    USGS Publications Warehouse

    Andrews, Freeman L.; Wells, Frank C.; Shelby, Wanda J.; McPherson, Emma

    1988-01-01

    Water-quality data collected from Lake Austin and Town Lake, following runoff, generally were not adequate to fully determine the effects of runoff on the lakes. Data collection should not to be limited to fixed-station sampling following runoff, and both lakes need to be sampled simultaneously as soon as possible following significant precipitation.

  7. Bathymetry of Bonnie Doone Lake, Kornbow Lake, Mintz Pond, and Glenville Lake, Cumberland County, North Carolina, 1996-98

    USGS Publications Warehouse

    Giorgino, M.J.; Strain, R.E.

    1999-01-01

    Bathymetric surveys were conducted at four water-supply impoundments of Little Cross Creek in Cumberland County, North Carolina. The surveys were conducted in April 1996 at Mintz Pond and Glenville Lake, and in January 1998 at Bonnie Doone Lake and Kornbow Lake. The resulting bathymetric maps are the first to cover the entire range in depth for these reservoirs and provide a framework for future evaluations of bathymetry and storage capacity. Bathymetric maps were constructed from depth and position data collected at each reservoir. A boat-mounted, research-grade fathometer was used to record water depths with a vertical accuracy of 0.1 foot. At Mintz Pond and Glenville Lake, position was measured by using a wide-band laser tracking system interfaced with a total station survey instrument. This positioning method required multiple land-based control points to be established and was hampered by line-of-sight restrictions between the control points and the boat. At Bonnie Doone Lake and Kornbow Lake, a global positioning system was used to collect differentially corrected location data. This positioning method enabled more rapid data collection, eliminated the need for land-based control points, and provided improved data coverage. Spillway elevations range from 172.8 feet above mean sea level at Bonnie Doone Lake to 113.1 feet at Glenville Lake. Surface area and storage volume were computed for each reservoir and were related to water-surface elevations at 1-foot intervals. The combined surface acreage of the four Little Cross Creek reservoirs at their full-pool elevations is 120.97 acres, consisting of 21.20 acres at Bonnie Doone Lake, 47.09 acres at Kornbow Lake, 15.56 acres at Mintz Pond, and 37.12 acres at Glenville Lake. The four reservoirs have a combined usable storage capacity of 674.91 acre-feet, which is the sum of 127.93 acre-feet in Bonnie Doone Lake, 320.62 acre-feet in Kornbow Lake, 53.25 acre-feet in Mintz Pond, and 173.11 acre-feet in Glenville Lake.

  8. Lake trout in the Great Lakes: Basin-wide stock collapse and binational restoration

    USGS Publications Warehouse

    Hansen, Michael J.; Taylor, William W.; Ferreri, C. Paola

    1999-01-01

    The lake trout (Salvelinus namaycush) was important to the human settlement of each of the Great Lakes, and underwent catastrophic collapses in each lake in the nineteenth and twentieth centuries. The timing of lake trout stock collapses were different in each lake, as were the causes of the collapses, and have been the subject of much scientific inquiry and debate. The purpose of this chapter is to summarize and review pertinent information relating historical changes in Great Lakes lake trout stocks, binational efforts to restore those stocks, and progress toward stock restoration. This presentation attempts to generalize patterns across the Great Lakes, rather than to focus within each lake. Lake specific analyses have been used to understand lake specific causes and effects, but there is continuing debate about some of these causes and effects. A basinwide review may suggest mechanisms for observed changes that are not evident by lake specific analysis.

  9. GloboLakes: A global observatory of lake responses to environmental change.

    NASA Astrophysics Data System (ADS)

    Groom, Steve; Tyler, Andrew; Hunter, Peter; Spyrakos, Evangelos; Martinez-Vicente, Victor; Merchant, Chris; Cutler, Mark; Rowan, John; Dawson, Terry; Maberly, Stephen; Cavalho, Laurence; Elliot, Alex; Thackery, Stephen; Miller, Claire; Scott, Marian

    2014-05-01

    The world's freshwater ecosystems are vital components of the global biosphere, yet are vulnerable to climate and other human-induced change. There is increasing recognition that lakes play an important role in global biogeochemical cycling and provide key ecosystem services. However, our understanding of how lakes respond to environmental change at a global scale, and how this impacts on their status and function, is hampered by limited information on their chemical, physical and ecological condition. There are estimated to be over 300 million lakes globally, of which over 17,000 are greater than 10 km2 in surface area. These numbers have limited the systematic study of lake ecosystems. GloboLakes is a five-year UK research programme investigating the state of lakes and their response to climatic and other environmental drivers of change. It will establish a satellite-based observatory with archive and near-real time data processing to produce a time series of observed biogeochemical parameters and lake temperature for over 1000 lakes globally. This will be supported by linked ancillary data on climate and catchment land-use. The ability to monitor a large number of lakes consistently at high frequency and globally will facilitate a paradigm shift in our understanding of how lakes respond to environmental change at different spatial and temporal scales. A key requirement is to validate satellite retrieval algorithms and test the time-series of resulting lake properties such as chlorophyll-a by comparison with in situ data. To support the former extensive bio-optical and constituent data were taken in year 1 of the project in a number of UK lakes with a variety of trophic states. Furthermore, for wider validation activities GloboLakes has established the LIMNADES initiative to create a centralised database of ground bio-optical measurements of worldwide lakes through voluntary cooperation across the international scientific community. This presentation will

  10. Potential for water borne and invertebrate transmission of West Nile virus in the Great Salt Lake, Utah

    USGS Publications Warehouse

    Lund, Melissa; Shearn-Bochsler, Valerie I.; Dusek, Robert; Shivers, Jan; Hofmeister, Erik K.

    2017-01-01

    In November and December of 2013, a large mortality event involving 15,000 - 20,000 eared grebes (Podiceps nigricollis) occurred at the Great Salt Lake (GSL), UT. The onset of the outbreak in grebes was followed by a mortality event in > 86 bald eagles (Haliaeetus leucocephalus). During the die-off, West Nile virus (WNV) was detected by RT-PCR or viral culture in carcasses of grebes and eagles submitted to the National Wildlife Health Center. However, no mosquito activity, the primary vector of WNV, was detected by the State of Utah's WNV monitoring program. Transmission of WNV has rarely been reported during the winter in North America in the absence of known mosquito activity; however, the size of this die-off, the habitat in which it occurred, and the species involved are unique. We experimentally investigated whether WNV could survive in water with a high saline content, as found at the GSL, and whether brine shrimp, the primary food of migrating eared grebes on the GSL, could have played a role in transmission of WNV to feeding birds. We found that WNV can survive up to 72 h at 4°C in water containing 30 — 150 ppt NaCl and brine shrimp, incubated with WNV in 30 ppt NaCl, may adsorb WNV to their cuticle and, through feeding, may infect epithelial cells of their gut. Both mechanisms may have potentiated the WNV die-off in migrating eared grebes on the GSL.

  11. Reintroduction of H5N1 highly pathogenic avian influenza virus by migratory water birds, causing poultry outbreaks in the 2010-2011 winter season in Japan.

    PubMed

    Sakoda, Yoshihiro; Ito, Hiroshi; Uchida, Yuko; Okamatsu, Masatoshi; Yamamoto, Naoki; Soda, Kosuke; Nomura, Naoki; Kuribayashi, Saya; Shichinohe, Shintaro; Sunden, Yuji; Umemura, Takashi; Usui, Tatsufumi; Ozaki, Hiroichi; Yamaguchi, Tsuyoshi; Murase, Toshiyuki; Ito, Toshihiro; Saito, Takehiko; Takada, Ayato; Kida, Hiroshi

    2012-03-01

    H5N1 highly pathogenic avian influenza virus (HPAIV) was reintroduced and caused outbreaks in chickens in the 2010-2011 winter season in Japan, which had been free from highly pathogenic avian influenza (HPAI) since 2007 when HPAI outbreaks occurred and were controlled. On 14 October 2010 at Lake Ohnuma, Wakkanai, the northernmost part of Hokkaido, Japan, H5N1 HPAIVs were isolated from faecal samples of ducks flying from their nesting lakes in Siberia. Since then, in Japan, H5N1 HPAIVs have been isolated from 63 wild birds in 17 prefectures and caused HPAI outbreaks in 24 chicken farms in nine prefectures by the end of March in 2011. Each of these isolates was genetically closely related to the HPAIV isolates at Lake Ohnuma, and those in China, Mongolia, Russia and Korea, belonging to genetic clade 2.3.2.1. In addition, these isolates were genetically classified into three groups, suggesting that the viruses were transmitted by migratory water birds through at least three different routes from their northern territory to Japan. These isolates were antigenic variants, which is consistent with selection in poultry under the immunological pressure induced by vaccination. To prevent the perpetuation of viruses in the lakes where water birds nest in summer in Siberia, prompt eradication of HPAIVs in poultry is urgently needed in Asian countries where HPAI has not been controlled.

  12. Molecular epidemiology reveals emergence of a virulent infectious hematopoietic necrosis (IHN) virus strain in wild salmon and its transmission to hatchery fish

    USGS Publications Warehouse

    Anderson, Eric D.; Engelking, H. Mark; Emmenegger, Eveline J.; Kurath, Gael

    2000-01-01

    Infectious hematopoietic necrosis virus (IHNV) has been known to be a significant salmonid pathogen in the Pacific Northwest of North America for decades. The goal of this study was to characterize the IHNV genetic heterogeneity and viral traffic over time at a study site in the Deschutes River watershed in Oregon, with an emphasis on the epidemiology of IHNV types causing epidemics in wild kokanee Oncorhynchus nerkabetween 1991 and 1995. The study site included kokanee spawning grounds in the Metolius River and Lake Billy Chinook downstream, in which the IHNV epidemics occurred in 2- and 3-year-old kokanee, and the Round Butte Fish Hatchery at the outflow of the lake. Forty-two IHNV isolates collected from this area between 1975 and 1995 were characterized on a genetic basis by ribonuclease (RNase) protection fingerprint analyses of the virus nucleocapsid, glycoprotein, and nonvirion genes. Analysis of the 16 identified composite haplotypes suggested that both virus evolution and introduction of new IHNV strains contributed to the genetic diversity observed. The results indicated that the 1991–1995 epidemics in kokanee from Lake Billy Chinook were due to a newly introduced IHNV type that was first detected in spawning adult kokanee in 1988 and that this virus type was transmitted from the wild kokanee to hatchery fish downstream in 1991. Twelve IHNV haplotypes were found at Round Butte Fish Hatchery, indicating a series of virus displacement events during the 20-year period examined. This work shows that IHNV traffic can be much more complex than was previously recognized, and the results have implications for fisheries management at the hatchery and throughout the watershed.

  13. Variable viral and grazer control of prokaryotic growth efficiency in temperate freshwater lakes (French Massif Central).

    PubMed

    Ram, A S Pradeep; Palesse, S; Colombet, J; Sabart, M; Perriere, F; Sime-Ngando, T

    2013-11-01

    The effects of viral lysis and heterotrophic nanoflagellate grazing (top down forces) on prokaryotic mortality and their subsequent impact on their metabolism were estimated in the upper euphotic and deeper aphotic depth of 11 freshwater lakes located in the French Massif Central. The standing stocks of viruses (VA) and heterotrophic nanoflagellate (HNF) varied significantly (p < 0.05) with sampled depth. VA was substantially (twofold on an average) and significantly higher (p < 0.03) at the aphotic compared to euphotic depth, whereas the reverse was true (p < 0.02) for HNF. Among the prokaryote subgroup, high nucleic acid content prokaryotes explained for significant variability in the total VA and served as principle host target for viral proliferation. Like standing stocks, flagellate grazing and viral infection rates also followed similar patterns. In the investigated lakes, the mechanism for regulating prokaryotic production varied with sampled depth from grazing control in the euphotic to control due to viral lysis in the aphotic. We also tested the hypothesis of top down control on prokaryotic growth efficiency (PGE, which we used as an index of prokaryotic physiological and energetic status at the community level) at both depths. Overall, among the studied lakes, PGE varied widely (4-51 %) with significantly (p < 0.05) lower values in the aphotic (mean = 18 ± 4 %) than euphotic depth (mean = 32 ± 9 %). Contrasting observations on the top down control of PGE between sampled depths were observed. The presence of grazers was found to stimulate PGE at the euphotic, whereas viruses through their lytic infection had a strong negative impact on PGE at the aphotic depth. Such observed differences in PGE and the mechanism controlling prokaryotic production with depth could eventually have strong implication on carbon and nutrient flux patterns in the studied lakes.

  14. Watershed vs. within-lake drivers of nitrogen: phosphorus dynamics in shallow lakes.

    PubMed

    Ginger, Luke J; Zimmer, Kyle D; Herwig, Brian R; Hanson, Mark A; Hobbs, William O; Small, Gaston E; Cotner, James B

    2017-10-01

    Research on lake eutrophication often identifies variables affecting amounts of phosphorus (P) and nitrogen (N) in lakes, but understanding factors influencing N:P ratios is important given its influence on species composition and toxin production by cyanobacteria. We sampled 80 shallow lakes in Minnesota (USA) for three years to assess effects of watershed size, proportion of watershed as both row crop and natural area, fish biomass, and lake alternative state (turbid vs. clear) on total N : total P (TN : TP), ammonium, total dissolved phosphorus (TDP), and seston stoichiometry. We also examined N:P stoichiometry in 20 additional lakes that shifted states during the study. Last, we assessed the importance of denitrification by measuring denitrification rates in sediment cores from a subset of 34 lakes, and by measuring seston δ 15 N in four additional experimental lakes before and after they were experimentally manipulated from turbid to clear states. Results showed alternative state had the largest influence on overall N:P stoichiometry in these systems, as it had the strongest relationship with TN : TP, seston C:N:P, ammonium, and TDP. Turbid lakes had higher N at given levels of P than clear lakes, with TN and ammonium 2-fold and 1.4-fold higher in turbid lakes, respectively. In lakes that shifted states, TN was 3-fold higher in turbid lakes, while TP was only 2-fold higher, supporting the notion N is more responsive to state shifts than is P. Seston δ 15 N increased after lakes shifted to clear states, suggesting higher denitrification rates may be important for reducing N levels in clear states, and potential denitrification rates in sediment cores were among the highest recorded in the literature. Overall, our results indicate lake state was a primary driver of N:P dynamics in shallow lakes, and lakes in clear states had much lower N at a given level of P relative to turbid lakes, likely due to higher denitrification rates. Shallow lakes are often

  15. Changes in Rongbuk lake and Imja lake in the Everest region of Himalaya

    NASA Astrophysics Data System (ADS)

    Chen, W.; Doko, T.; Liu, C.; Ichinose, T.; Fukui, H.; Feng, Q.; Gou, P.

    2014-12-01

    The Himalaya holds the world record in terms of range and elevation. It is one of the most extensively glacierized regions in the world except the Polar Regions. The Himalaya is a region sensitive to climate change. Changes in the glacial regime are indicators of global climate changes. Since the second half of the last century, most Himalayan glaciers have melted due to climate change. These changes directly affected the changes of glacial lakes in the Himalayan region due to the glacier retreat. New glacial lakes are formed, and a number of them have expanded in the Everest region of the Himalayas. This paper focuses on the two glacial lakes which are Imja Lake, located at the southern slope, and Rongbuk Lake, located at the northern slope in the Mt. Everest region, Himalaya to present the spatio-temporal changes from 1976 to 2008. Topographical conditions between two lakes were different (Kruskal-Wallis test, p < 0.05). Rongbuk Lake was located at 623 m higher than Imja Lake, and radiation of Rongbuk Lake was higher than the Imja Lake. Although size of Imja Lake was larger than the Rongbuk Lake in 2008, the growth speed of Rongbuk Lake was accelerating since 2000 and exceeds Imja Lake in 2000-2008. This trend of expansion of Rongbuk Lake is anticipated to be continued in the 21st century. Rongbuk Lake would be the biggest potential risk of glacial lake outburst flood (GLOF) at the Everest region of Himalaya in the future.

  16. 78 FR 17097 - Safety Zone; Lake Havasu Triathlon; Lake Havasu City, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-20

    ...-AA00 Safety Zone; Lake Havasu Triathlon; Lake Havasu City, AZ AGENCY: Coast Guard, DHS. ACTION... waters of Lake Havasu and the London Bridge Channel for the Lake Havasu Triathlon. This temporary safety... participants. The waterside swim course consists of 1500 meters in Lake Havasu and the London Bridge Channel...

  17. Competition between larval lake herring (Coregonus artedi) and lake whitefish (Coregonus clupeaformis) for zooplankton

    USGS Publications Warehouse

    Davis, Bruce M.; Todd, Thomas N.

    1998-01-01

    Diet and growth of larval lake herring (Coregonus artedi) and lake whitefish (Coregonus clupeaformis) were compared in mesocosm experiments in a small mesotrophic lake in southeastern Michigan. Fish were sampled from single-species and mixed assemblages in 2-m3 cages for 8 weeks during April and May. Both species initially ate mostly cyclopoid copepodites and small cladocerans (Bosmia spp.). Schoener's index of diet overlap showed considerable overlap (70-90%). Lake whitefish ate Daphnia spp. and adult copepods about 2 weeks earlier than did lake herring, perhaps related to their larger mean mouth gape. Lake whitefish were consistently larger than lake herring until the eighth week, especially in the sympatric treatments. Lake whitefish appeared to have a negative effect on the growth of lake herring, as lake herring in mixed-species treatments were smaller and weighed less than lake herring reared in single-species treatments. The diet similarities of lake whitefish and lake herring larvae could make them competitors for food in the Great Lakes. The greater initial size of lake whitefish could allow them to eat larger prey earlier and thereby limit availability of these prey to lake herring at a crucial period of development.

  18. The Morphometry of Lake Palmas, a Deep Natural Lake in Brazil

    PubMed Central

    Barroso, Gilberto F.; Gonçalves, Monica A.; Garcia, Fábio da C.

    2014-01-01

    Lake Palmas (A = 10.3km2) is located in the Lower Doce River Valley (LDRV), on the southeastern coast of Brazil. The Lake District of the LDRV includes 90 lakes, whose basic geomorphology is associated with the alluvial valleys of the Barreiras Formation (Cenozoic, Neogene) and with the Holocene coastal plain. This study aimed to investigate the relationship of morphometry and thermal pattern of a LDRV deep lake, Lake Palmas. A bathymetric survey carried out in 2011 and the analysis of hydrographic and wind data with a geographic information system allowed the calculation of several metrics of lake morphometry. The vertical profiling of physical and chemical variables in the water column during the wet/warm and dry/mild cold seasons of 2011 to 2013 has furnished a better understanding of the influence of the lake morphometry on its structure and function. The overdeepened basin has a subrectangular elongated shape and is aligned in a NW-SE direction in an alluvial valley with a maximum depth (Zmax) of 50.7m, a volume of 2.2×108 m3 (0.22km3) and a mean depth (Zmv) of 21.4m. These metrics suggest Lake Palmas as the deepest natural lake in Brazil. Water column profiling has indicated strong physical and chemical stratification during the wet/warm season, with a hypoxic/anoxic layer occupying one-half of the lake volume. The warm monomictic pattern of Lake Palmas, which is in an accordance to deep tropical lakes, is determined by water column mixing during the dry and mild cold season, especially under the influence of a high effective fetch associated with the incidence of cold fronts. Lake Palmas has a very long theoretical retention time, with a mean of 19.4 years. The changes observed in the hydrological flows of the tributary rivers may disturb the ecological resilience of Lake Palmas. PMID:25406062

  19. Embryotoxicity of Great Lakes lake trout extracts to developing rainbow trout

    USGS Publications Warehouse

    Wright, Peggy J.; Tillitt, Donald E.

    1999-01-01

    Planar halogenated hydrocarbons (PHHs), such as polychlorinated dibenzo-p-dioxins, dibenzofurans, and biphenyls are present in aquatic systems, and are known to produce adverse effects in fish. This study investigated the embryotoxicity of PHH mixtures through the nanoinjection of environmental extracts into newly fertilized eggs from two strains of rainbow trout. Organic extracts were obtained from whole adult lake trout collected from Lake Michigan in 1988 and Lake Superior in 1994. The graded doses of the final extracts used for injection were quantified as 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic-equivalents (TEQs) based on the concentrations of dioxins, furans and non-o-PCBs in each, and as equivalent amounts found in the eggs of the original lake trout (eggEQ). Total TEQs in the lake trout were 14.7 pg TEQ/g in the Lake Michigan sample and 7.3 pg TEQ/g in the Lake Superior sample. The extract of the Lake Michigan lake trout was embryotoxic to rainbow trout; LD50 values were 35 eggEQ (15–90, 95% F.L.) in the Arlee strain and 14 eggEQ (5–99, 95% F.L.) in the Erwin strain of rainbow trout. The LD50 values of the Lake Michigan extract in either of these strains of rainbow trout fall within the actual range of TCDD LD50values based on TEQs. This indicates that an additive model of toxicity is appropriate to quantify PHHs in relation to early life stage mortality in fish. Gross lesions characteristic of exposure to PHHs (i.e. yolk-sac edema, craniofacial deformities, and hemorrhaging) increased in a dose-related manner. The lowest observable adverse effect concentrations (LOAEC) for these gross lesions and cumulative mortalities suggests that current concentrations of PHHs in lake trout from Lake Michigan are above a threshold for adverse effects and these compounds may have implications on the lack of recruitment in certain Great Lakes lake trout populations.

  20. Great Lakes

    NASA Image and Video Library

    2017-12-08

    Bands of lake effect snow drift eastward from the western Great Lakes in this true-color image captured by the NOAA/NASA Suomi NPP satellite's Visible Infrared Imaging Radiometer Suite (VIIRS) instrument on January 5, 2017. National Weather Service forecasters expect light to moderate lake effect snow showers to continue throughout the day today and into Saturday (1/7). Lake-effect snow forms when cold air passes over the warmer waters of a lake. This causes some lake water to evaporate into the air and warm it. This warmer, wetter air rises and cools as it moves away from the lake. When it cools, it releases that moisture and, if it’s cold enough, that moisture turns into snow. Although true-color images like this may appear to be photographs of Earth, they aren't. They are created by combining data from the three color channels on the VIIRS instrument sensitive to the red, green and blue (or RGB) wavelengths of light into one composite image. In addition, data from several other channels are often also included to cancel out or correct atmospheric interference that may blur parts of the image. Credit: NOAA/NASA/Suomi NPP via NOAA's Environmental Visualization Laboratory

  1. Lake Michigan lake trout PCB model forecast post audit

    EPA Science Inventory

    Scenario forecasts for total PCBs in Lake Michigan (LM) lake trout were conducted using the linked LM2-Toxics and LM Food Chain models, supported by a suite of additional LM models. Efforts were conducted under the Lake Michigan Mass Balance Study and the post audit represents th...

  2. Water-quality and lake-stage data for Wisconsin lakes, water year 2005

    USGS Publications Warehouse

    Rose, W.J.; Garn, H.S.; Goddard, G.L.; Marsh, S.B.; Olson, D.L.; Robertson, Dale M.

    2006-01-01

    The U.S. Geological Survey (USGS), in cooperation with local and other agencies, collects data at selected lakes throughout Wisconsin. These data, accumulated over many years, provide a data base for developing an improved understanding of the water quality of lakes. The purpose of this report is to provide information about the chemical and physical charac-teristics of Wisconsin lakes. Data that have been collected at specific lakes, and information to aid in the interpretation of those data, are included in this report. Data collected include measure-ments of in-lake water quality and lake stage. Time series graphs of Secchi depths, surface total phosphorus and chlorophyll a concentrations collected during non-frozen periods are included for all lakes. Graphs of vertical profiles of temperature, dissolved oxygen, pH, and specific conductance are included for sites where these parameters were measured. Descriptive infor-mation for each lake includes: location of the lake, area of the lake's watershed, period for which data are available, revisions to previously published records, and pertinent remarks.

  3. Using Satellite Imagery to Monitor the Major Lakes; Case Study Lake Hamun

    NASA Astrophysics Data System (ADS)

    Norouzi, H.; Islam, R.; Bah, A.; AghaKouchak, A.

    2015-12-01

    Proper lakes function can ease the impact of floods and drought especially in arid and semi-arid regions. They are important environmentally and can directly affect human lives. Better understanding of the effect of climate change and human-driven changes on lakes would provide invaluable information for policy-makers and local people. As part of a comprehensive study, we aim to monitor the land-cover/ land-use changes in the world's major lakes using satellite observations. As a case study, Hamun Lake which is a pluvial Lake, also known as shallow Lake, located on the south-east of Iran and adjacent to Afghanistan, and Pakistan borders is investigated. The Lake is the main source of resources (agriculture, fishing and hunting) for the people around it and politically important in the region since it is shared among three different countries. The purpose of the research is to find the Lake's area from 1972 to 2015 and to see if any drought or water resources management has affected the lake. Analyzing satellites imagery from Landsat shows that the area of the Lake changes seasonally and intra-annually. Significant seasonal effects are found in 1975,1977, 1987, 1993, 1996, 1998, 2000, 2009 and 2011, as well as, substantial amount of shallow water is found throughout the years. The precipitation records as well as drought historical records are studied for the lake's basin. Meteorological studies suggest that the drought, decrease of rainfalls in the province and the improper management of the Lake have caused environmental, economic and geographical consequences. The results reveal that lake has experienced at least two prolong dryings since 1972 which drought cannot solely be blamed as main forcing factor.Proper lakes function can ease the impact of floods and drought especially in arid and semi-arid regions. They are important environmentally and can directly affect human lives. Better understanding of the effect of climate change and human-driven changes on lakes

  4. A coupled lake-atmosphere model (CLAM) and its application to Lake Kinneret

    NASA Astrophysics Data System (ADS)

    Pan, Hai

    1999-08-01

    Kinneret is a 166-km2 lake located in Northern Israel, in the central part of the Jordan Valley, a corridor running from north to south, between the Galilee hills in the west and the Golan Heights in the east. Both the Galilee hills and the Golan Heights reach an elevation of about 400 m above mean sea level (MSL), and the lake is about -210 m (MSL). North of the lake is the mountainous area of the Hermon, culminating at about 2800 m (MSL). About 120 km south of it is the Dead Sea, which is about -410 m (MSL), and about 45 km west of it is the Mediterranean Sea. The complexity of the terrain, combined with relatively arid soil and various ground covers surrounding the lake, results in a very complicated system of atmospheric and lake processes. To understand this system, especially the processes affecting the atmosphere and lake dynamics and thermodynamics, and their effects on Lake Kinneret evaporation, a coupled lake-atmosphere model (CLAM) was developed and applied to the lake region. The CLAM is based on the Regional Atmospheric Modeling System (RAMS) and the oceanic S-coordinate Rutgers University Model (SCRUM). Energy, mass, and momentum are conserved at the interface between the atmosphere and the lake, and appropriate balance equations are applied there. In the atmospheric module, two nested grids are employed to simulate Northern Israel at a resolution of 4 x 4 km2, and the near-lake region at a resolution of 1 x 1 km 2. Synoptic conditions obtained from the National Meteorological Center (NMC) reanalysis are assimilated by the model. Soil moisture, which appears to have a significant impact on atmospheric circulation in this region, was transformed from the normalized difference vegetation index (NDVI). Observations collected during two summers above and inside the lake emphasize the good capability of CLAM to simulate surface fluxes and other microclimatic conditions, as well as lake temperature and currents. Although the lake is small (about 12-km wide

  5. Bathymetric Surveys of Lake Arthur and Raccoon Lake, Pennsylvania, June 2007

    USGS Publications Warehouse

    Hittle, Clinton D.; Ruby, A. Thomas

    2008-01-01

    In spring of 2007, bathymetric surveys of two Pennsylvania State Park lakes were performed to collect accurate data sets of lake-bed elevations and to develop methods and techniques to conduct similar surveys across the state. The lake-bed elevations and associated geographical position data can be merged with land-surface elevations acquired through Light Detection and Ranging (LIDAR) techniques. Lake Arthur in Butler County and Raccoon Lake in Beaver County were selected for this initial data-collection activity. In order to establish accurate water-surface elevations during the surveys, benchmarks referenced to NAVD 88 were established on land at each lake by use of differential global positioning system (DGPS) surveys. Bathymetric data were collected using a single beam, 210 kilohertz (kHz) echo sounder and were coupled with the DGPS position data utilizing a computer software package. Transects of depth data were acquired at predetermined intervals on each lake, and the shoreline was delineated using a laser range finder and compass module. Final X, Y, Z coordinates of the geographic positions and lake-bed elevations were referenced to NAD 83 and NAVD 88 and are available to create bathymetric maps of the lakes.

  6. Comparison of catch and lake trout bycatch in commercial trap nets and gill nets targeting lake whitefish in northern Lake Huron

    USGS Publications Warehouse

    Johnson, James E.; Ebener, Mark P.; Gebhardt, Kenneth; Bergstedt, Roger

    2004-01-01

    We compared seasonal lake whitefish catch rates, lake trout bycatch, and gearinduced lake trout mortality between commercial trap nets and gill nets in north-central Lake Huron. Onboard monitors recorded catches from 260 gill net and 96 trap net lifts from October 1998 through December 1999. Catch rates for lake whitefish were highest in fall for both gear types, reflecting proximity of spawning sites to the study area. Lake whitefish catch rates were also relatively high in spring but low in both gear types in summer. Lake trout were the principal bycatch species in both gears. The lake trout bycatch was lowest in both gear types in fall, highest in gill nets in spring, and highest in trap nets in summer. The ratio of lake trout to legal whitefish (the target species) was highest in summer and lowest in fall in both gear types. The high lake trout ratio in summer was due principally to low catch rates of lake whitefish. All but 3 of 186 live lake trout removed from trap net pots survived for at least two days of observation in laboratory tanks. Therefore, we estimated that post-release survival of trap netted lake trout that had not been entangled in the mesh was 98.4%. In addition, we accounted for stress-induced mortality for lake trout that were live at capture but entangled in the mesh of either gear type. Resulting estimates of lake trout survival were higher in trap nets (87.8%) than in gill nets (39.6%). The number of lake trout killed per lift was highest during summer in trap nets and during spring in gill nets. In trap nets, 85% of dead lake trout were observed to be entangled in the mesh of the pot or tunnels. Survival rates of lake trout in gill nets were higher in our study than reported by others, probably because our nets were hand lifted in a small boat. Our trap net-induced mortality estimates on lake trout were higher than those reported by others because we adjusted our estimates to account for post-release mortality caused by handling and

  7. Lake levels, streamflow, and surface-water quality in the Devils Lake area, North Dakota

    USGS Publications Warehouse

    Wiche, Gregg J.

    1996-01-01

    The Devils Lake Basin is a 3,810-square-mile (mi2) closed basin (fig. 1) in the Red River of the North Basin. About 3,320 mi2 of the total 3,810 mi2 is tributary to Devils Lake; the remainder is tributary to Stump Lake.Since glaciation, the lake level of Devils Lake has fluctuated from about 1,457 feet (ft) above sea level (asl), the natural spill elevation of the lake to the Sheyenne River, to 1,400 ft asl (Aronow, 1957). Although no documented records of lake levels are available before 1867, Upham (1895, p. 595), on the basis of tree-ring chronology, indicated that the lake level was 1,441 ft asl in 1830. Lake levels were recorded sporadically from 1867 to 1901 when the U.S. Geological Survey established a gaging station on Devils Lake. From 1867 to the present (1996), the lake level has fluctuated between a maximum of 1,438.4 ft asl in 1867 and a minimum of 1,400.9 ft asl in 1940 (fig. 2). On July 31, 1996, the lake level was 1,437.8 ft asl, about 15.2 ft higher than the level recorded in February 1993 and the highest level in about 120 years.Since 1993, the lake level of Devils Lake (fig. 2) has risen rapidly in response to above-normal precipitation from the summer of 1993 to the present, and 30,000 acres of land around the lake have been flooded. The above-normal precipitation also has caused flooding elsewhere in the Devils Lake Basin. State highways near Devils Lake are being raised, and some local roads have been closed because of flooding.In response to the flooding, the Devils Lake Basin Interagency Task Force, comprised of many State and Federal agencies, was formed in 1995 to find and propose intermediate (5 years or less) solutions to reduce the effects of high lake levels. In addition to various planning studies being conducted by Federal agencies, the North Dakota State Water Commission has implemented a project to store water on small tracts of land and in the chain of lakes (Sweetwater Lake, Morrison Lake, Dry Lake, Mikes Lake, Chain Lake

  8. Genetic diversity of Diporeia in the Great Lakes: comparison of Lake Superior to the other Great Lakes

    EPA Science Inventory

    Abundances of Diporeia have dropped drastically in the Great Lakes, except in Lake Superior, where data suggest that population counts actually have risen. Various ecological, environmental, or geographic hypotheses have been proposed to explain the greater abundance of Lake Supe...

  9. Water-quality and lake-stage data for Wisconsin lakes, water year 1996

    USGS Publications Warehouse

    ,

    1997-01-01

    The purpose of this report is to provide information about the physical and chemical characteristics of Wisconsin lakes. Data that have been collected at specific lakes, and information to aid in the interpretation of those data, are included in this report. Data collected includes measurements of lake stage and in-lake water quality. Graphs of Secchi depths, surface totalphosphorus and chlorophyll-a concentrations versus time are included for lakes with two or more years of data. Graphs of vertical profiles of temperature, dissolved oxygen, pH, and specific conductance are included for sites where these parameters were measured. Descriptive information for each lake includes: location of the lake, drainage area of the lake's watershed, period for which data are available, revisions to previously published records, and pertinent remarks. Additional data, such as streamflow and water quality in tributary and outlet streams of some of the lakes, are published in another volume: "Water Resources Data-Wisconsin, 1996."

  10. New data on the unresolved paradox of the Tibesti crater paleolakes (Central Sahara, North Chad)

    NASA Astrophysics Data System (ADS)

    Kroepelin, S.; Darius, F.; Deschamps, P.; Dinies, M.; Hoelzmann, P.; Kuper, J.; Oppenheimer, C.; Soulié-Märsche, I.; Sylvestre, F.

    2015-12-01

    Recent field work in the volcanic Tibesti Mountains opens a new chapter in the reconstruction of the last climatic cycles in the central Sahara and their lacustrine environments. For the first time, complete lacustrine sections were sampled in the 900 m deep crater of Trou au Natron at Pic Toussidé (3,315 m a.s.l.), and in 800 m deep Era Kohor, the major sub-caldera of Emi Koussi, the Sahara's 3,445 m high peak (photo). The probed diatomites are located 360 and 125 m above the present-day bottom of the calderas. Studies in the 1960s suggested that lake levels in the Trou au Natron were 300-500 m high at 12,400-14,970 uncal. yrs BP. Such lake depths and resulting water volumes, however, are hardly conceivable in view of the limited intake area and precipitation-evaporation ratios which would have required local rainfall by far surpassing estimates for latitudes 19-21°N from the full-Holocene record of Lake Yoa situated 460 or 220 km southeast, and 1,550 or 2,450 m lower. The presentation will examine whether differences in altitude may explain unparalleled lake depths and postglacial humid conditions 4,000-7,000 years earlier than in the surrounding lowlands, and present alternative hypotheses of lake formation in this Saharan key region.

  11. Delineation of sympatric morphotypes of lake trout in Lake Superior

    USGS Publications Warehouse

    Moore, Seth A.; Bronte, Charles R.

    2001-01-01

    Three morphotypes of lake trout Salvelinus namaycush are recognized in Lake Superior: lean, siscowet, and humper. Absolute morphotype assignment can be difficult. We used a size-free, whole-body morphometric analysis (truss protocol) to determine whether differences in body shape existed among lake trout morphotypes. Our results showed discrimination where traditional morphometric characters and meristic measurements failed to detect differences. Principal components analysis revealed some separation of all three morphotypes based on head and caudal peduncle shape, but it also indicated considerable overlap in score values. Humper lake trout have smaller caudal peduncle widths to head length and depth characters than do lean or siscowet lake trout. Lean lake trout had larger head measures to caudal widths, whereas siscowet had higher caudal peduncle to head measures. Backward stepwise discriminant function analysis retained two head measures, three midbody measures, and four caudal peduncle measures; correct classification rates when using these variables were 83% for leans, 80% for siscowets, and 83% for humpers, which suggests the measures we used for initial classification were consistent. Although clear ecological reasons for these differences are not readily apparent, patterns in misclassification rates may be consistent with evolutionary hypotheses for lake trout within the Laurentian Great Lakes.

  12. LakeMIP Kivu: Evaluating the representation of a large, deep tropical lake by a set of 1-dimensional lake models

    NASA Astrophysics Data System (ADS)

    Thiery, Wim; Stepanenko, Viktor; Darchambeau, François; Joehnk, Klaus; Martynov, Andrey; Mironov, Dmitrii; Perroud, Marjorie; van Lipzig, Nicole

    2013-04-01

    The African great lakes are of utmost importance for the local economy (fishing), as well as being essential to the survival of the local people. During the last decades, these lakes experienced fast changes in ecosystem structure and functioning and their future evolution is a major concern. In this study, for the first time a set of one-dimensional lake models are evaluated over East-Africa, in particular over Lake Kivu (2.28 °S; 28.98 °E). The unique limnology of meromictic Lake Kivu, with the importance of salinity and geothermal springs in a tropical high-altitude climate, presents a worthy challenge to the 1D-lake models currently involved in the Lake Model Intercomparison Project (LakeMIP). Furthermore, this experiment will serve as the basis for a future, more complex intercomparison, coupling lake models with atmospheric circulation models to analyse climate change effects on the lake. Meteorological observations from two automatic weather stations, one at Kamembe airport (Rwanda, 2003-2008), the other at ISP Bukavu (DRC, 2003-2011), are used to drive each of these models. For the evaluation, a unique dataset is used which contains over 150 temperature profiles recorded since 2002. The standard LakeMIP protocol is adapted to mirror the limnological conditions in Lake Kivu and to unify model parameters as far as possible. Since some lake models do not account for salinity and its effect upon lake stratification, two sets of simulations are performed with each model: one for the freshwater layer only (60 m) and one for the average lake depth (240 m) including salinity. Therewith, on the one hand it is investigated whether each model is able to reproduce the correct mixing regime in Lake Kivu and captures the controlling of this seasonality by the relative humidity, which constrains evaporation except during summer (JJA). On the other hand, the ability of different models to simulate salinity- and geothermal-induced effects upon deep water stratification is

  13. Planetary Lake Lander - A Robotic Sentinel to Monitor a Remote Lake

    NASA Technical Reports Server (NTRS)

    Pedersen, Liam; Smith, Trey; Lee, Susan; Cabrol, Nathalie; Rose, Kevin

    2012-01-01

    The Planetary Lake Lander Project is studying the impact of rapid deglaciation at a high altitude alpine lake in the Andes, where disrupted environmental, physical, chemical, and biological cycles result in newly emerging natural patterns. The solar powered Lake Lander robot is designed to monitor the lake system and characterize both baseline characteristics and impacts of disturbance events such as storms and landslides. Lake Lander must use an onboard adaptive science-on-the-fly approach to return relevant data about these events to mission control without exceeding limited energy and bandwidth resources. Lake Lander carries weather sensors, cameras and a sonde that is winched up and down the water column to monitor temperature, dissolved oxygen, turbidity and other water quality parameters. Data from Lake Lander is returned via satellite and distributed to an international team of scientists via web-based ground data systems. Here, we describe the Lake Lander Project scientific goals, hardware design, ground data systems, and preliminary data from 2011. The adaptive science-on-the-fly system will be described in future papers.

  14. Molecular characterization of measles viruses that circulated in Cameroon between 2010 and 2011.

    PubMed

    Demanou, Maurice; Ratsitoharana, Rajhonson; Yonga, Martial; Dosseh, Annick; Anya, Blanche; Kobela, Marie; Njouom, Richard

    2013-03-04

    Measles virus (MeV) is monotypic, but genetic variation in the hemagglutinin H and nucleoprotein N genes can be analyzed by molecular epidemiologic techniques and used to study virus transmission patterns. The World Health Organization currently recognizes 8 clades (A-H) within which are 24 genotypes of MeV and one provisional genotype, d11. Genotype B3 is clearly the endemic genotype in most of African continent where it is widely distributed. We provide an update on the molecular characterization of wild-type MeVs that circulated in Cameroon between 2010 and 2011. Viral RNA was extracted directly from samples obtained from clinically diagnosed measles patients using QIAamp viral RNA Mini Kit. Reverse transcription and PCR amplification of 634 nucleotides of the N gene was performed using the SuperScript™ III One-Step. Sequence analysis of 450 of the 634 nucleotides using Clustal X 2.0 program for multiple alignments and Mega version 5 for phylogenic analysis indicated that all the viruses belonged to genotype B3 with two distinct clusters. Twenty three (77%) belonged to subgroup B3.1 and the other 7 (23%) belonged to B3.3 a recently described subtype. Circulation of cluster 3 was detected in the Far-North Region (5/7) particularly along the Chad-Cameroon border in 2010 and later in Yaounde (2/7 in Biyem-assi Health District) the capital city of Cameroon in 2011. This study highlights the endemic circulation in Cameroon of MeV B3 subtype 1, which probably has its source in the neighboring Nigeria, and the presence of the new subtype B3.3, suggesting a possible importation from Northern Africa where it was first described between 2008 and 2009.

  15. Patterns of organochlorine contamination in lake trout from Wisconsin waters of the Great Lakes

    USGS Publications Warehouse

    Miller, Michael A.; Madenjian, Charles P.; Masnado, Robert G.

    1992-01-01

    To investigate spatial and temporal patterns of organochlorine contamination in lake trout from Wisconsin waters of the Great Lakes, we examined laboratory contaminant analysis data of muscle tissue samples from Lake Michigan (n=317) and Lake Superior (n=53) fish. Concentrations of polychlorinated biphenyls (PCBs), chlordane, and dieldrin, reported as mg/kg wet weight in 620 mm to 640 mm mean length Lake Michigan lake trout, decreased over time. Mean total PCB concentration declined exponentially from 9.7 in 1975 to 1.9 in 1990. Total chlordane concentration declined 63 percent from 0.48 in 1983 to 0.18 in 1990, and dieldrin declined 52 percent during this same period, from 0.21 to 0.10. The bioaccumulation rate of PCBs is significantly lower for lake trout inhabiting Lake Michigan's midlake reef complex, compared to lake trout from the nearshore waters of western Lake Michigan. Organochlorine compound concentrations were greater in Lake Michigan lake trout than Lake Superior fish. Lake Superior lean lake trout and siscowet exhibited similar rates of PCB bioaccumulation despite major differneces in muscle tissue lipid content between the two subspecies. The lack of a significant difference in the PCB bioaccumulation rates of lean trout and siscowet suggests that lipid content may not be an important factor influencing PCB bioaccumulation in lake trout, within the range of lipid concentrations observed. Relative concentrations of the various organochlorine contaminants found in lake trout were highly correlated, suggesting similar mass balance processes for these compounds. Evidence presented revealing spatial and temporal patterns of organochlorine contamination may be of value in reestablishing self-sustaining populations of lake trout in Lake Michigan.

  16. Winter diet of lake herring (Coregonus artedi) in western Lake Superior

    USGS Publications Warehouse

    Link, Jason; Selgeby, James H.; Hoff, Michael H.; Haskell, Craig

    1995-01-01

    Lake herring (Coregonus artedi) and zooplankton samples were simultaneously collected through the ice in the Apostle Islands region of western Lake Superior to provide information on the winter feeding ecology of lake herring. Zooplankton constituted the entire diet of the 38 lake herring collected for this study. We found no evidence of piscivory, although it has been reported by anglers. Diet selectivities were calculated using a Wilcoxon signed-ranks test and showed a preference of lake herring for larger zooplankton, especially Diaptomus sicilis, whereas the smaller copepod,Cyclops bicuspidatus thomasi, and immature copepod stages were selected against. These data document that overwintering copepods are food for a broad size range of lake herring in winter.

  17. Mono Lake, California

    NASA Image and Video Library

    2017-03-24

    In eastern California, along the western edge of the Great Basin, sits Mono Lake. This is a salty remnant of a wetter era. Estimates are that the lake existed for at least 760,000 years. Now surrounded by mountain ranges, however, Mono Lake has no outlet; water entering the lake can only evaporate away, so Mono Lake is saltier than the ocean. South of the lake appear some of the geologic features known as Mono Craters. Geologists estimate that the Mono Craters last erupted about 650 years ago. The image was acquired July 7, 2016, covers an area of 22.6 by 34 km, and is located at 37.9 degrees north, 119 degrees west. http://photojournal.jpl.nasa.gov/catalog/PIA21518

  18. Synthetic Musk Fragrances in Lake Erie and Lake Ontario Sediment Cores

    PubMed Central

    Peck, Aaron M.; Linebaugh, Emily K.; Hornbuckle, Keri C.

    2009-01-01

    Two sediment cores collected from Lake Ontario and Lake Erie were sectioned, dated, and analyzed for five polycyclic musk fragrances and two nitro musk fragrances. The polycyclic musk fragrances were HHCB (Galaxolide), AHTN (Tonalide), ATII (Traseolide), ADBI (Celestolide), and AHMI (Phantolide). The nitro musk fragrances were musk ketone and musk xylene. Chemical analysis was performed by gas chromatography/mass spectrometry (GC/MS) and results from Lake Erie were confirmed using gas chromatography/triple-quadrupole mass spectrometry (GC/MS/MS). The chemical signals observed at the two sampling locations were different from each other due primarily to large differences in the sedimentation rates at the two sampling locations. HHCB was detected in the Lake Erie core while six compounds were detected in the Lake Ontario core. Using measured fragrance and 210Pb activity, the burden of synthetic musk fragrances estimated from these sediment cores is 1900 kg in Lake Erie and 18000 kg in Lake Ontario. The input of these compounds to the lakes is increasing. The HHCB accumulation rates in Lake Erie for 1979-2003 and 1990-2003 correspond to doubling times of 16 ± 4 yr and 8 ± 2 yr, respectively. The results reflect current U.S. production trends for the sum of all fragrance compounds. PMID:17007119

  19. Status of lake trout rehabilitation in the Northern Refuge of Lake Michigan

    USGS Publications Warehouse

    Madenjian, Charles P.; DeSorcie, Timothy J.

    1999-01-01

    The Northern Refuge in Lake Michigan was established in 1985 as part of a rehabilitation program to stock yearling lake trout Salvelinus namaycush in areas with the best potential for success. Stocking of hatchery-reared lake trout within the refuge began in 1986 at three reefs: Boulder Reef, Gull Island Reef, and Richards Reef. On each reef from 1991 to 1997 we conducted gill-net surveys during the fall spawning season to evaluate performance of adult lake trout, and we conducted beam trawl surveys for naturally reproduced age-0 lake trout in the spring. Criteria to evaluate performance included spawner density, growth, maturity, and mortality. We found no evidence of natural reproduction by lake trout from our surveys. Nevertheless, density of spawning lake trout on Boulder Reef (69 fish/305 m of gill net/night) and Gull Island Reef (34 fish/305 m of gill net/night) appeared to be sufficiently high to initiate a self-sustaining population. Growth and maturity rates of lake trout in the Northern Refuge were similar to those for lake trout stocked in the nearshore region of Lake Michigan. In the Northern Refuge, growth rate for the Marquette strain of lake trout was slightly higher than for the Lewis Lake strain. Annual mortality estimates from catch curve analyses ranged from 0.46 to 0.41, and therefore, these estimates approached a level that was considered to be sufficiently low to allow for a self-sustaining population. Thus, it appeared that the lack of evidence for natural reproduction by lake trout in the Northern Refuge should not be attributed to inability of the population to attain a sufficiently large stock of spawners.

  20. Water-Quality and Lake-Stage Data for Wisconsin Lakes, Water Year 2007

    USGS Publications Warehouse

    Wisconsin Water Science Center Lake-Studies Team: Rose, W. J.; Garn, H.S.; Goddard, G.L.; Marsh, S.B.; Olson, D.L.; Robertson, Dale M.

    2008-01-01

    The U.S. Geological Survey (USGS), in cooperation with local and other agencies, collects data at selected lakes throughout Wisconsin. These data, accumulated over many years, provide a data base for developing an improved understanding of the water quality of lakes. To make these data available to interested parties outside the USGS, the data are published annually in this report series. The locations of water-quality and lake-stage stations in Wisconsin for water year 2007 are shown in figure 1. A water year is the 12-month period from October 1 through September 30. It is designated by the calendar year in which it ends. Thus, the period October 1, 2005 through September 30, 2007 is called 'water year 2007.' The purpose of this report is to provide information about the chemical and physical characteristics of Wisconsin lakes. Data that have been collected at specific lakes, and information to aid in the interpretation of those data, are included in this report. Data collected include measurements of in-lake water quality and lake stage. Time series of Secchi depths, surface total phosphorus and chlorophyll a concentrations collected during non-frozen periods are included for all lakes. Graphs of vertical profiles of temperature, dissolved oxygen, pH, and specific conductance are included for sites where these parameters were measured. Descriptive information for each lake includes: location of the lake, area of the lake?s watershed, period for which data are available, revisions to previously published records, and pertinent remarks. Additional data, such as streamflow and water quality in tributary and outlet streams of some of the lakes, are published in another volume: 'Water Resources Data-Wisconsin, 2007.'

  1. 42. Peaks of Otter, Abbott Lake. View across lake to ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    42. Peaks of Otter, Abbott Lake. View across lake to peaks of Outter Lodge, completed in 1964. Construction of the lake got underway in 1964. Looking east-northeast. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  2. Patterns of egg deposition by lake trout and lake whitefish at Tawas artificial Reef, Lake Huron, 1990-1993

    USGS Publications Warehouse

    Foster, N.R.; Kennedy, G.W.; Munawar, M.; Edsall, T.; Leach, J.

    1995-01-01

    In August 1987, the Michigan Department of Natural Resources (MDNR), with the help and co-sponsorship of Walleyes for Iosco County, constructed Tawas artificial reef to improve recreational fishing in Tawas Bay. Post-construction assessment in October, 1987, by the MDNR found twice as many adult lake trout in a gill net set on the reef as in a similar net set off the reef, indicating that lake trout already had begun to investigate this new habitat. Similar netting efforts in October 1989 caught three times as many adults on the reef as off it, even though the on-reef net was set for less than one third as long a period. Using a remotely operated vehicle (ROV), we detected prespawning aggregations of lake trout on the reef in fall 1989, and MDNR biologists set emergent fly traps on the reef in April-May 1990-1991. These fry traps captured several newly emerged lake trout and lake whitefish fry, demonstrating that eggs of both species has hatched successfully. Gill netting in 1992-1993 by U.S. Fish and Wildlife Service biologists netted large numbers of ripe lake trout in late October and ripe lake whitefish in early to mid-November. The purpose of this paper is to describe the relative quantities of eggs deposited and the spatial patterns of egg deposition by lake trout and lake whitefish at Tawas artificial reef during 1990-1993.

  3. Lake Powell

    NASA Image and Video Library

    2007-09-20

    The white ring around Lake Powell tells the story. The surface is down 98 feet. This is critical, because Powell, Lake Mead, and other lakes along the Colorado River provide water for millions of people in five states. We are in the eighth year of a drought on the Colorado River. This year was the driest year ever reported in Southern California, and there is a severe drought in Northern California, down to less than 30-percent of snow pack. This ASTER image of part of Lake Powell was acquired in 2001. The gray area depicts the shrunken, reduced 2007 lake extent compared to the extended, larger black area in 2001. The image covers an area of 24 x 30 km, and is centered near 37.1 degrees north latitude, 111.3 degrees west longitude. This image from NASA Terra satellite. http://photojournal.jpl.nasa.gov/catalog/PIA10614

  4. Evaluation of an Experimental Re-introduction of Sockeye Salmon into Skaha Lake; Year 2 of 3, 2001 Technical Report.

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Fisher, Christopher; Machin, Deanna; Wright, Howie

    This report summarizes the findings from YEAR 2 of a three-year disease risk assessment. The Okanagan Nation Fisheries Commission (ONFC) and the Colville Confederated Tribes (CCT) are investigating the risks involved in re-introducing sockeye salmon into Skaha Lake, part of their historical range (Ernst and Vedan 2000). The disease risk assessment compares the disease and infection status of fish above and below McIntyre Dam (the present limit of sockeye migration). The disease agents identified that are of a particular concern are: infectious pancreatic necrosis virus (IPNV), infectious haematopoietic necrosis virus type 2 (IHNV type2), erythrocytic inclusion body syndrome virus (EIBSV),more » the whirling disease agent (Myxobolus cerebralis), and the ceratomyxosis agent (Ceratomyxa shasta).« less

  5. National Lakes Assessment: A Collaborative Survey of the Nation's Lakes

    EPA Science Inventory

    The National Lakes Assessment A Collaborative Survey of the Nation's Lakes presents the results of an unprecedented assessment of the nation’s lakes. This report is part of the National Aquatic Resource Surveys, a series of statistically based surveys designed to provide the pub...

  6. Characterization of a Novel Orthomyxo-like Virus Causing Mass Die-Offs of Tilapia

    PubMed Central

    Bacharach, Eran; Mishra, Nischay; Briese, Thomas; Zody, Michael C.; Kembou Tsofack, Japhette Esther; Zamostiano, Rachel; Berkowitz, Asaf; Ng, James; Nitido, Adam; Corvelo, André; Toussaint, Nora C.; Abel Nielsen, Sandra Cathrine; Hornig, Mady; Del Pozo, Jorge; Bloom, Toby; Ferguson, Hugh

    2016-01-01

    ABSTRACT Tilapia are an important global food source due to their omnivorous diet, tolerance for high-density aquaculture, and relative disease resistance. Since 2009, tilapia aquaculture has been threatened by mass die-offs in farmed fish in Israel and Ecuador. Here we report evidence implicating a novel orthomyxo-like virus in these outbreaks. The tilapia lake virus (TiLV) has a 10-segment, negative-sense RNA genome. The largest segment, segment 1, contains an open reading frame with weak sequence homology to the influenza C virus PB1 subunit. The other nine segments showed no homology to other viruses but have conserved, complementary sequences at their 5′ and 3′ termini, consistent with the genome organization found in other orthomyxoviruses. In situ hybridization indicates TiLV replication and transcription at sites of pathology in the liver and central nervous system of tilapia with disease. PMID:27048802

  7. Actinomadura gamaensis sp. nov., a novel actinomycete isolated from soil in Gama, Chad.

    PubMed

    Abagana, Adam Yacoub; Sun, Pengyu; Liu, Chongxi; Cao, Tingting; Zheng, Weiwei; Zhao, Shanshan; Xiang, Wensheng; Wang, Xiangjing

    2016-06-01

    A novel single spore-producing actinomycete, designated strain NEAU-Gz5(T), was isolated from a soil sample from Gama, Chad. A polyphasic taxonomic study was carried out to establish the status of this strain. The diamino acid present in the cell wall is meso-diaminopimelic acid. Glucose, mannose and madurose occur in whole cell hydrolysates. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and an unidentified glycolipid. The predominant menaquinones were identified as MK-9(H8) and MK-9(H6). The predominant cellular fatty acids were found to be C16:0, iso-C15:0, iso-C16:0 and C18:0 10-methyl. Phylogenetic analysis based on the 16S rRNA gene showed that strain NEAU-Gz5(T) belongs to the genus Actinomadura and is closely related to Actinomadura oligospora JCM 10648(T) (ATCC 43269(T); 98.3 % similarity). However, the low level of DNA-DNA relatedness and some different phenotypic characteristics allowed the strain to be distinguished from its close relatives. Therefore, it is concluded that strain NEAU-Gz5(T) represents a novel species of the genus of Actinomadura, for which the name Actinomadura gamaensis sp. nov. is proposed. The type strain is NEAU-Gz5(T) (= CGMCC 4.7301(T) = DSM 100815(T)).

  8. Hydrology of the Lake Deaton and Lake Okahumpka area, Northeast Sumter County, Florida

    USGS Publications Warehouse

    Simonds, Edward P.; German, E.R.

    1980-01-01

    The Floridan aquifer in the Lake Deaton and Lake Okahumpka area is 50 to 130 feet below land surface. During the 16-year period 1963-78 lake evaporation exceeded rainfall by 0.4 inches. Drainage from Lake Deaton and its surrounding area goes into Chitty Chatty Creek and on the Hogeye Sink when the altitude of the potentiometric surface of the Floridan aquifer is low. During a higher altitude of the Floridan potentiometric surface, Hogeye Sink may discharge water; this water, along with the normal runoff, goes into Lake Okahumpka. Average lake fluctuation is 1.5 to 2.0 feet per year. Lake Deaton supports a large population of blue-green algae and Lake Okahumpka is choked with aquatic plants. The water quality of the two lakes differ, with Lake Deaton having a sodium chloride water and Lake Okahumpka having a calcium bicarbonate water. Analysis of water and bottom material samples showed that only cadmium and mercury exceeded the Florida Department of Environmental Regulation 's criteria for Class III waters; however, the amounts detected were at or slightly above the limits of the analytical method. (USGS)

  9. Strand-plain evidence for late Holocene lake-level variations in Lake Michigan

    USGS Publications Warehouse

    Thompson, T.A.; Baedke, S.J.

    1997-01-01

    Lake level is a primary control on shoreline behavior in Lake Michigan. The historical record from lake-level gauges is the most accurate source of information on past lake levels, but the short duration of the record does not permit the recognition of long-term patterns of lake-level change (longer than a decade or two). To extend the record of lake-level change, the internal architecture and timing of development of five strand plains of late Holocene beach ridges along the Lake Michigan coastline were studied. Relative lake-level curves for each site were constructed by determining the elevation of foreshore (swash zone) sediments in the beach ridges and by dating basal wetland sediments in the swales between ridges. These curves detect long-term (30+ yr) lake-level variations and differential isostatic adjustments over the past 4700 yr at a greater resolution than achieved by other studies. The average timing of beach-ridge development for all sites is between 29 and 38 yr/ridge. This correspondence occurs in spite of the embayments containing the strand plains being different in size, orientation, hydrographic regime, and available sediment type and caliber. If not coincidental, all sites responded to a lake-level fluctuation of a little more than three decades in duration and a range of 0.5 to 0.6 m. Most pronounced in the relative lake-level curves is a fluctuation of 120-180 yr in duration. This ???150 yr variation is defined by groups of four to six ridges that show a rise and fall in foreshore elevations of 0.5 to 1.5 m within the group. The 150 yr variation can be correlated between sites in the Lake Michigan basin. The ???30 and 150 yr fluctuations are superimposed on a long-term loss of water to the Lake Michigan basin and differential rates of isostatic adjustment.

  10. Water-Quality and Lake-Stage Data for Wisconsin Lakes, Water Year 2006

    USGS Publications Warehouse

    Rose, W.J.; Garn, H.S.; Goddard, G.L.; Marsh, S.B.; Olson, D.L.; Robertson, Dale M.

    2007-01-01

    The U.S. Geological Survey (USGS), in cooperation with local and other agencies, collects data at selected lakes throughout Wisconsin. These data, accumulated over many years, provide a data base for developing an improved understanding of the water quality of lakes. To make these data available to interested parties outside the USGS, the data are published annually in this report series. The locations of water-quality and lake-stage stations in Wisconsin for water year 2006 are shown in figure 1. A water year is the 12-month period from October 1 through September 30. It is designated by the calendar year in which it ends. Thus, the period October 1, 2005 through September 30, 2006 is called 'water year 2006.' The purpose of this report is to provide information about the chemical and physical characteristics of Wisconsin lakes. Data that have been collected at specific lakes, and information to aid in the interpretation of those data, are included in this report. Data collected include measurements of in-lake water quality and lake stage. Time series of Secchi depths, surface total phosphorus and chlorophyll a concentrations collected during non-frozen periods are included for all lakes. Graphs of vertical profiles of temperature, dissolved oxygen, pH, and specific conductance are included for sites where these parameters were measured. Descriptive information for each lake includes: location of the lake, area of the lake's watershed, period for which data are available, revisions to previously published records, and pertinent remarks. Additional data, such as streamflow and water quality in tributary and outlet streams of some of the lakes, are published in another volume: 'Water Resources Data-Wisconsin, 2006.' Water-resources data, including stage and discharge data at most streamflow-gaging stations, are available through the World Wide Web on the Internet. The Wisconsin Water Science Center's home page is at http://wi.water.usgs.gov/. Information on the

  11. Diet and prey selection by Lake Superior lake trout during springs 1986-2001

    USGS Publications Warehouse

    Ray, B.A.; Hrabik, T.R.; Ebener, M.P.; Gorman, O.T.; Schreiner, D.R.; Schram, S.T.; Sitar, S.P.; Mattes, W.P.; Bronte, C.R.

    2007-01-01

    We describe the diet and prey selectivity of lean (Salvelinus namaycush namaycush) and siscowet lake trout (S. n. siscowet) collected during spring (April–June) from Lake Superior during 1986–2001. We estimated prey selectivity by comparing prey numerical abundance estimates from spring bottom trawl surveys and lake trout diet information in similar areas from spring gill net surveys conducted annually in Lake Superior. Rainbow smelt (Osmerus mordax) was the most common prey and was positively selected by both lean and siscowet lake trout throughout the study. Selection by lean lake trout for coregonine (Coregonus spp.) prey increased after 1991 and corresponded with a slight decrease in selection for rainbow smelt. Siscowet positively selected for rainbow smelt after 1998, a change that was coincident with the decrease in selection for this prey item by lean lake trout. However, diet overlap between lean and siscowet lake trout was not strong and did not change significantly over the study period. Rainbow smelt remains an important prey species for lake trout in Lake Superior despite declines in abundance.

  12. Biology, population structure, and estimated forage requirements of lake trout in Lake Michigan

    USGS Publications Warehouse

    Eck, Gary W.; Wells, LaRue

    1983-01-01

    Data collected during successive years (1971-79) of sampling lake trout (Salvelinus namaycush) in Lake Michigan were used to develop statistics on lake trout growth, maturity, and mortality, and to quantify seasonal lake trout food and food availability. These statistics were then combined with data on lake trout year-class strengths and age-specific food conversion efficiencies to compute production and forage fish consumption by lake trout in Lake Michigan during the 1979 growing season (i.e., 15 May-1 December). An estimated standing stock of 1,486 metric tons (t) at the beginning of the growing season produced an estimated 1,129 t of fish flesh during the period. The lake trout consumed an estimated 3,037 t of forage fish, to which alewives (Alosa pseudoharengus) contributed about 71%, rainbow smelt (Osmerus mordax) 18%, and slimy sculpins (Cottus cognatus) 11%. Seasonal changes in bathymetric distributions of lake trout with respect to those of forage fish of a suitable size for prey were major determinants of the size and species compositions of fish in the seasonal diet of lake trout.

  13. Lakes and lake-like waters of the Hawaiian Archipelago

    USGS Publications Warehouse

    Maciolek, J.A.

    1982-01-01

    This summary of Hawaiian lacustrine limnology is based on 12 years of field and literature surveys of archipelagic inland waters. Lakes here are distinguished from other standing waters by limits on surface oceanic area (> 0.1 ha) and depth (> 2 m), and by the absence of flatural surface oceanic connection. A variety of extinct and existing water bodies, sometimes referred to as lakes, are noted. Six lakes are described. Five of them are in crater basins, 3 are freshwater, and 2 are elevated (highest = 3969 m). The scarcity of elevated lakes results from general permeability of the substrata. Among the 6 lakes, surface areas range from 0.22 to 88 ha and maximum depths from 3 to 248 m. Naturally occurring aquatic biota generally is low in species diversity except for phytoplankton; fishes and submersed vascular plants are absent. Two lowland lakes, freshwater Green (Wai a Pele) and saline Kauhak6, are described for the first time. Profundal Kauhak6, 248 m deep, has a surface area of only 0.35 ha, which results in an extraordinary relative depth of 370%. It is permanently stratified, a condition apparently due primarily to the unique morphometry of its basin. 

  14. Hypothesis on the source, transmission and characteristics of infection of avian influenza A (H7N9) virus--based on analysis of field epidemiological investigation and gene sequence analysis.

    PubMed

    Ling, F; Chen, E; Liu, Q; Miao, Z; Gong, Z

    2015-02-01

    On 31 March 2013, the National Health and Family Planning Commission announced that human infections with influenza A (H7N9) virus had occurred in Shanghai and Anhui provinces, China. H7N9 cases were later detected in Jiangsu and Zhejiang provinces. It was estimated that the virus first spread northward along the route taken by migratory birds and then spread to neighbouring provinces with the sale of poultry. Epidemiological studies were carried out on samples from the external environment of infected cases, transmission routes, farmers markets and live poultry markets. Phylogenetic study of viral sequences from human and avian infections in Zhejiang showed that those from Shanghai and Jiangsu provinces along Taihu Lake were highly homologous with those from the external environment. This suggests that avian viruses carried by waterfowl combined with the virus carried by migratory birds, giving rise to avian influenza virus H7N9, which is highly pathogenic to humans. It is possible that the virus was transmitted by local wildfowl to domestic poultry and then to humans, or spread further by means of trading in wholesale poultry markets. As the weather has turned warm, and with measures adopted to terminate poultry trade and facilitate health communication, the epidemic in the first half of the year has been kept under control. However, the infection source in the triangular area around Taihu Lake still remains. The H7N9 epidemic will probably hit the area later in the year and next spring when the migratory birds return and may even spread to other areas. Great importance should therefore be attached to the wildfowl in Taihu Lake as the repository and disseminator of the virus: investigation and study of this population is essential. © 2014 Blackwell Verlag GmbH.

  15. Lake Powell

    NASA Technical Reports Server (NTRS)

    2007-01-01

    The white ring around Lake Powell tells the story. The surface is down 98 feet. This is critical, because Powell, Lake Mead, and other lakes along the Colorado River provide water for millions of people in five states. We are in the eighth year of a drought on the Colorado River. This year was the driest year ever reported in Southern California, and there is a severe drought in Northern California, down to less than 30-percent of snow pack. This ASTER image of part of Lake Powell was acquired in 2001. The gray area depicts the shrunken, reduced 2007 lake extent compared to the extended, larger black area in 2001.

    The image covers an area of 24 x 30 km, and is centered near 37.1 degrees north latitude, 111.3 degrees west longitude.

    The U.S. science team is located at NASA's Jet Propulsion Laboratory, Pasadena, Calif. The Terra mission is part of NASA's Science Mission Directorate.

  16. Ciscoes (Coregonus, subgenus Leucichthys) of the Laurentian Great Lakes and Lake Nipigon

    USGS Publications Warehouse

    Eshenroder, Randy L.; Vecsei, Paul; Gorman, Owen T.; Yule, Daniel; Pratt, Thomas C.; Mandrak, Nicholas E.; Bunnell, David B.; Muir, Andrew M.

    2016-01-01

    This study of the ciscoes (Coregonus, subgenus Leucichthys) of the Great Lakes and Lake Nipigon represents a furtherance through 2015 of field research initiated by Walter Koelz in 1917 and continued by Stanford Smith in the mid-1900s—a period spanning nearly a century. Like Koelz’s study, this work contains information on taxonomy, geographical distribution, ecology, and status of species (here considered forms). Of the seven currently recognized forms (C. artedi, C. hoyi, C. johannae, C. kiyi, C. nigripinnis, C. reighardi, and C. zenithicus) described by Koelz as major in his 1929 monograph, two (C. johannae and C. reighardi) are extinct. In addition, C. alpenae, described by Koelz but subsequently synonymized with C. zenithicus, although extinct, is recognized as valid making a total of eight major forms. Six of these forms, all but C. artedi and C. hoyi, have been lost from Lake Michigan, and seven have been lost from Lake Huron, leaving in Lake Huron only C. artedi and an introgressed deepwater form that we term a hybrid swarm. C. artedi appears, like its sister form C. alpenae, to have been lost from Lake Erie. Only C. artedi remains extant in Lake Ontario, its three sister forms (C. hoyi, C. kiyi, and C. reighardi) having disappeared long ago.Lakes Superior and Nipigon have retained their original species flocks consisting of four forms each: C. artedi, C. hoyi, and C. zenithicus in both lakes; C. kiyi in Lake Superior; and C. nigripinnis in Lake Nipigon. Morphological deviations from the morphotypes described by Koelz have been modest in contemporary samples. Overall, C. kiyi and C. artedi were the most morphologically stable forms while C. hoyi, C. nigripinnis, and C. zenithicus were the least stable. Although contemporary populations of C. artedi from Lakes Michigan and Huron are highly diverged from the morphotypes described by Koelz, the contemporary samples were of undescribed deep-bodied forms unlikely to have been sampled by Koelz because of

  17. Sedimentary constraints on late Quaternary lake-level fluctuations at Bear Lake, Utah and Idaho

    USGS Publications Warehouse

    Smoot, J.P.; Rosenbaum, J.G.

    2009-01-01

    A variety of sedimentological evidence was used to construct the lake-level history for Bear Lake, Utah and Idaho, for the past ???25,000 years. Shorelines provide evidence of precise lake levels, but they are infrequently preserved and are poorly dated. For cored sediment similar to that in the modern lake, grain-size distributions provide estimates of past lake depths. Sedimentary textures provide a highly sensitive, continuous record of lake-level changes, but the modern distribution of fabrics is poorly constrained, and many ancient features have no modern analog. Combining the three types of data yields a more robust lake-level history than can be obtained from any one type alone. When smooth age-depth models are used, lake-level curves from multiple cores contain inconsistent intervals (i.e., one record indicates a rising lake level while another record indicates a falling lake level). These discrepancies were removed and the multiple records were combined into a single lake-level curve by developing age-depth relations that contain changes in deposition rate (i.e., gaps) where indicated by sedimentological evidence. The resultant curve shows that, prior to 18 ka, lake level was stable near the modern level, probably because the lake was overflowing. Between ca. 17.5 and 15.5 ka, lake level was ???40 m below the modern level, then fluctuated rapidly throughout the post-glacial interval. Following a brief rise centered ca. 15 ka ( = Raspberry Square phase), lake level lowered again to 15-20 m below modern from ca. 14.8-11.8 ka. This regression culminated in a lowstand to 40 m below modern ca. 12.5 ka, before a rapid rise to levels above modern ca. 11.5 ka. Lake level was typically lower than present throughout the Holocene, with pronounced lowstands 15-20 m below the modern level ca. 10-9, 7.0, 6.5-4.5, 3.5, 3.0-2.5, 2.0, and 1.5 ka. High lake levels near or above the modern lake occurred ca. 8.5-8.0, 7.0-6.5, 4.5-3.5, 2.5, and 0.7 ka. This lake-level history

  18. Fat content of the flesh of siscowets and lake trout from Lake Superior

    USGS Publications Warehouse

    Eschmeyer, Paul H.; Phillips, Arthur M.

    1965-01-01

    Samples of flesh were excised from the middorsal region of 67 siscowets (Salvelinus namaycush siscowet) and 46 lake trout (Salvelinus n. namaycush) collected from Lake Superior. Chemical analysis of the samples revealed a range in fat content (dry weight) of 32.5 to 88.8 per cent in siscowets and 6.6 to 52.3 per cent in lake trout. Percentage fat increased progressively with increase in length of fish in both forms, but the average rate of increase was far greater for siscowets than for lake trout at lengths between 12 and 20 inches. Despite substantial individual variation, the percentage fat in the two forms was widely different and without overlap at all comparable lengths. The range in iodine number of the fat was 100 to 160 for siscowets and 103 to 161 for lake trout; average values were generally lower for siscowets than for lake trout among fish of comparable length. Percentage fat and relative weight were not correlated significantly in either subspecies. The fat content of flesh samples from a distinctive subpopulation of Lake Superior lake trout known as 'humpers' was more closely similar to that of typical lean lake trout than to siscowets, but the rate of increase in fat with increasing length was greater than for lean lake trout. Flesh samples from hatchery-reared stocks of lake trout, hybrid lake trout X siscowets, and siscowets tended to support the view that the wide difference in fat content between siscowets and lake trout is genetically determined.

  19. 77 FR 9652 - Proposed CERCLA Administrative Cost Recovery Settlement; Lake Linden Superfund Site in Lake...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-17

    ... Settlement; Lake Linden Superfund Site in Lake Linden, Houghton County, MI AGENCY: Environmental Protection... concerning the Lake Linden Superfund Site in Lake Linden, Houghton County, Michigan with Honeywell Specialty...-6609. Comments should reference the Lake Linden Superfund Site in Lake Linden, Houghton County...

  20. Highly pathogenic avian influenza virus (H5N1) isolated from whooper swans, Japan.

    PubMed

    Uchida, Yuko; Mase, Masaji; Yoneda, Kumiko; Kimura, Atsumu; Obara, Tsuyoshi; Kumagai, Seikou; Saito, Takehiko; Yamamoto, Yu; Nakamura, Kikuyasu; Tsukamoto, Kenji; Yamaguchi, Shigeo

    2008-09-01

    On April 21, 2008, four whooper swans were found dead at Lake Towada, Akita prefecture, Japan. Highly pathogenic avian influenza virus of the H5N1 subtype was isolated from specimens of the affected birds. The hemagglutinin (HA) gene of the isolate belongs to clade 2.3.2 in the HA phylogenetic tree.

  1. Long-term simulations of dissolved oxygen concentrations in Lake Trout lakes

    NASA Astrophysics Data System (ADS)

    Jabbari, A.; Boegman, L.; MacKay, M.; Hadley, K.; Paterson, A.; Jeziorski, A.; Nelligan, C.; Smol, J. P.

    2016-02-01

    Lake Trout are a rare and valuable natural resource that are threatened by multiple environmental stressors. With the added threat of climate warming, there is growing concern among resource managers that increased thermal stratification will reduce the habitat quality of deep-water Lake Trout lakes through enhanced oxygen depletion. To address this issue, a three-part study is underway, which aims to: analyze sediment cores to understand the past, develop empirical formulae to model the present and apply computational models to forecast the future. This presentation reports on the computational modeling efforts. To this end, a simple dissolved oxygen sub-model has been embedded in the one-dimensional bulk mixed-layer thermodynamic Canadian Small Lake Model (CSLM). This model is currently being incorporated into the Canadian Land Surface Scheme (CLASS), the primary land surface component of Environment Canada's global and regional climate modelling systems. The oxygen model was calibrated and validated by hind-casting temperature and dissolved oxygen profiles from two Lake Trout lakes on the Canadian Shield. These data sets include 5 years of high-frequency (10 s to 10 min) data from Eagle Lake and 30 years of bi-weekly data from Harp Lake. Initial results show temperature and dissolved oxygen was predicted with root mean square error <1.5 °C and <3 mgL-1, respectively. Ongoing work is validating the model, over climate-change relevant timescales, against dissolved oxygen reconstructions from the sediment cores and predicting future deep-water temperature and dissolved oxygen concentrations in Canadian Lake Trout lakes under future climate change scenarios. This model will provide a useful tool for managers to ensure sustainable fishery resources for future generations.

  2. A Dynamical Downscaling study over the Great Lakes Region Using WRF-Lake: Historical Simulation

    NASA Astrophysics Data System (ADS)

    Xiao, C.; Lofgren, B. M.

    2014-12-01

    As the largest group of fresh water bodies on Earth, the Laurentian Great Lakes have significant influence on local and regional weather and climate through their unique physical features compared with the surrounding land. Due to the limited spatial resolution and computational efficiency of general circulation models (GCMs), the Great Lakes are geometrically ignored or idealized into several grid cells in GCMs. Thus, the nested regional climate modeling (RCM) technique, known as dynamical downscaling, serves as a feasible solution to fill the gap. The latest Weather Research and Forecasting model (WRF) is employed to dynamically downscale the historical simulation produced by the Geophysical Fluid Dynamics Laboratory-Coupled Model (GFDL-CM3) from 1970-2005. An updated lake scheme originated from the Community Land Model is implemented in the latest WRF version 3.6. It is a one-dimensional mass and energy balance scheme with 20-25 model layers, including up to 5 snow layers on the lake ice, 10 water layers, and 10 soil layers on the lake bottom. The lake scheme is used with actual lake points and lake depth. The preliminary results show that WRF-Lake model, with a fine horizontal resolution and realistic lake representation, provides significantly improved hydroclimates, in terms of lake surface temperature, annual cycle of precipitation, ice content, and lake-effect snowfall. Those improvements suggest that better resolution of the lakes and the mesoscale process of lake-atmosphere interaction are crucial to understanding the climate and climate change in the Great Lakes region.

  3. Survival of lake trout eggs and fry reared in water from the upper Great Lakes

    USGS Publications Warehouse

    Mac, Michael J.; Edsall, Carol Cotant; Seelye, James G.

    1985-01-01

    As part of continuing studies of the reproductive failure of lake trout (Salvelinus namaycush) in Lake Michigan, we measured the survival of lake trout eggs and fry of different origins and reared in different environments. Eggs and milt were stripped from spawning lake trout collected in the fall of 1980 from southeastern Lake Michigan, northwestern Lake Huron, south central Lake Superior, and from hatchery brood stock. Eggs from all sources were incubated, and the newly hatched fry were reared for 139 days in lake water from each of the three upper Great Lakes and in well water. Survival of eggs to hatching at all sites was lowest for those from Lake Michigan (70% of fertilized eggs) and highest for eggs from Lake Superior (96%). Comparisons of incubation water from the different lakes indicated that hatching success of eggs from all sources was highest in Lake Huron water, and lowest in Lake Michigan water. The most notable finding was the nearly total mortality of fry from eggs of southeastern Lake Michigan lake trout. At all sites, the mean survival of Lake Michigan fry through 139 days after hatching was only 4% compared to near 50% for fry from the other three sources. In a comparison of the rearing sites, little influence of water quality on fry survival was found. Thus, the poor survival was associated with the source of eggs and sperm, not the water in which the fry were reared.

  4. Vertical transmission of honey bee viruses in a Belgian queen breeding program.

    PubMed

    Ravoet, Jorgen; De Smet, Lina; Wenseleers, Tom; de Graaf, Dirk C

    2015-03-14

    The Member States of European Union are encouraged to improve the general conditions for the production and marketing of apicultural products. In Belgium, programmes on the restocking of honey bee hives have run for many years. Overall, the success ratio of this queen breeding programme has been only around 50%. To tackle this low efficacy, we organized sanitary controls of the breeding queens in 2012 and 2014. We found a high quantity of viruses, with more than 75% of the egg samples being infected with at least one virus. The most abundant viruses were Deformed Wing Virus and Sacbrood Virus (≥40%), although Lake Sinai Virus and Acute Bee Paralysis Virus were also occasionally detected (between 10-30%). In addition, Aphid Lethal Paralysis Virus strain Brookings, Black Queen Cell Virus, Chronic Bee Paralysis Virus and Varroa destructor Macula-like Virus occurred at very low prevalences (≤5%). Remarkably, we found Apis mellifera carnica bees to be less infected with Deformed Wing Virus than Buckfast bees (p < 0.01), and also found them to have a lower average total number of infecting viruses (p < 0.001). This is a significant finding, given that Deformed Wing Virus has earlier been shown to be a contributory factor to winter mortality and Colony Collapse Disorder. Moreover, negative-strand detection of Sacbrood Virus in eggs was demonstrated for the first time. High pathogen loads were observed in this sanitary control program. We documented for the first time vertical transmission of some viruses, as well as significant differences between two honey bee races in being affected by Deformed Wing Virus. Nevertheless, we could not demonstrate a correlation between the presence of viruses and queen breeding efficacies.

  5. The most acidified Austrian lake in comparison to a neutralized mining lake

    PubMed Central

    Moser, Michael; Weisse, Thomas

    2011-01-01

    This study investigated two mining lakes located in the north of Lower Austria. These lakes arose 45 years ago when open cast lignite mining ceased. The lakes are separated by a 7-m wide dam. Due to the oxidation of pyrite, both lakes have been acidified and exhibit iron, sulphate, and heavy metal concentrations several orders of magnitude higher than in circumneutral lakes. The water column of both lakes is divided into two layers by a pronounced chemocline. The smaller mining lake (AML), with pH close to of 2.6, is the most acidic lake in Austria, whereas flooding with stream water and by drainage from the surrounding fields neutralized the adjacent larger pit lake. The goal of our study was to investigate the effect of flooding on its physical, chemical and biological properties, in comparison to the pristine AML. Even relative to other extremely acidic lakes, the flora and fauna in the AML was reduced and composed of only two flagellate, one ciliate, and one rotifer species. The simplified pelagic food web in the mixolimnion consisted of heterotrophic bacteria, the mixotrophic flagellates Chlamydomonas acidophila and Ochromonas sp., the ciliate Oxytricha sp., and the rotifer Cephalodella sp. The latter two are as yet undescribed new species. The heliozoan Actinophrys sp. that may act as top predator occurred only in low abundance. The euglenid Lepocinclis buetschlii formed a stable deep chlorophyll maximum (DCM) at 7 m depth. Highest cell numbers of L. buetschlii in the DCM exceeded 108 L−1. The neutralized mining lake harboured higher plankton diversity similar to that of natural circumneutral lakes. A peak of at least 16 different phytoplankton taxa was observed during summer. The zooplankton consisted of several copepod species, daphnids and other cladocerans, and at least six different rotifer species. Several fish species occurred in the neutralized lake. Although the effect of non-permanent flooding was largely sustainable, interannual fluctuations of

  6. In vivo and in vitro phenotypic differences between Great Lakes VHSV genotype IVb isolates with sequence types vcG001 and vcG002

    PubMed Central

    Imanse, Sierra M.; Cornwell, Emily R.; Getchell, Rodman G.; Kurath, Gael; Bowser, Paul R.

    2014-01-01

    Viral hemorrhagic septicemia virus (VHSV) is an aquatic rhabdovirus first recognized in farmed rainbow trout in Denmark. In the past decade, a new genotype of this virus, IVb was discovered in the Laurentian Great Lakes basin and has caused several massive die-offs in some of the 28 species of susceptible North American freshwater fishes. Since its colonization of the Great Lakes, several closely related sequence types within genotype IVb have been reported, the two most common of which are vcG001 and vcG002. These sequence types have different spatial distributions in the Great Lakes. The aim of this study was to determine whether the genotypic differences between representative vcG001 (isolate MI03) and vcG002 (isolate 2010-030 #91) isolates correspond to phenotypic differences in terms of virulence using both an in vitro and in vivo approach. In vitro infection of epithelioma papulosum cyprini (EPC), bluegill fry (BF-2), and Chinook salmon embryo (CHSE) cells demonstrated some differences in onset and rate of growth in EPC and BF-2 cells, without any difference in the quantity of RNA produced. In vivo infection of round gobies (Neogobius melanostomus) via immersion exposure to different concentrations of vcG001 or vcG002 caused a significantly greater mortality in round gobies exposed to 102 plaque forming units ml−1 of vcG001. These experiments suggest that there are phenotypic differences between Great Lakes isolates of VHSV genotype IVb. PMID:25722533

  7. In vivo and in vitro phenotypic differences between Great Lakes VHSV genotype IVb isolates with sequence types vcG001 and vcG002

    USGS Publications Warehouse

    Imanse, Sierra M.; Cornwell, Emily R.; Getchell, Rodman G.; Kurath, Gael; Bowser, Paul R.

    2014-01-01

    Viral hemorrhagic septicemia virus (VHSV) is an aquatic rhabdovirus first recognized in farmed rainbow trout in Denmark. In the past decade, a new genotype of this virus, IVb was discovered in the Laurentian Great Lakes basin and has caused several massive die-offs in some of the 28 species of susceptible North American freshwater fishes. Since its colonization of the Great Lakes, several closely related sequence types within genotype IVb have been reported, the two most common of which are vcG001 and vcG002. These sequence types have different spatial distributions in the Great Lakes. The aim of this study was to determine whether the genotypic differences between representative vcG001 (isolate MI03) and vcG002 (isolate 2010-030 #91) isolates correspond to phenotypic differences in terms of virulence using both in vitro and in vivo approaches. In vitro infection of epithelioma papulosum cyprini (EPC), bluegill fry (BF-2), and Chinook salmon embryo (CHSE) cells demonstrated some differences in onset and rate of growth in EPC and BF-2 cells, without any difference in the quantity of RNA produced. In vivo infection of round gobies (Neogobius melanostomus) via immersion exposure to different concentrations of vcG001 or vcG002 caused a significantly greater mortality in round gobies exposed to 102 plaque forming units ml− 1 of vcG001. These experiments suggest that there are phenotypic differences between Great Lakes isolates of VHSV genotype IVb.

  8. Seismic Data Reveal Lake-Level Changes in Lake Issyk-Kul, Kyrgyzstan

    NASA Astrophysics Data System (ADS)

    Gebhardt, C.; Spiess, V.; Keil, H.; Sauermilch, I.; Oberhänsli, H.; Abdrakhmatov, K.; De Batist, M. A.; Naudts, L.; De Mol, L.

    2013-12-01

    Lake Issyk-Kul is located in an intramontane basin of the Tien Shan, Kyrgyzstan, Central Asia, at 1607 m above sea level. It has formed in a tectonically active region with W-E striking major thrust zones both N and S of the lake. The lake is elongated with 180 km in W-E and 60 km in S-N direction and a water depth of roughly 670 m at its central plain. With a surface area of 6232 km2 and a total water colume of around 1736 km3, Lake Issyk-Kul is the second largest lake in the higher altitudes (De Batist et al., 2002). Two large delta areas have formed at the E and W end. Steep slopes at both the N and S shore separate rather narrow, shallow shelf areas from the central deeper plain. First seismic data of lake Issyk-Kul were acquired in 1982 by the Moscow University with a total of 31 profiles across the lake. In 1997 and 2001, a second and third seismic survey of the lake were carried out by the group of Marc De Batist (Ghent, Belgium) in cooperation with the Royal Museum of Central Africa (Tervuren, Belgium) and the SBRAS (Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia) using a sparker system with a single-channel streamer. These surveys were recently completed by a fourth expedition carried out by the University of Bremen in April 2013. During this expedition, 33 additional profiles were acquired with an airgun and a multi-channel streamer. The sparker surveys mostly cover the delta and shelf areas in high detail, while the airgun survey covers the deeper parts of the lake with penetration beyond the first multiple. Bathymetry data reveal that at the delta areas, the shelf is divided into two parts. The shallower comprises the part down to 110 m water depth with an average inclination of 0.5°, while the deeper part reaches from 110 m to 300 m water depth with an average slope inclination of 1°. Incised paleo-river channels of up to 2-3 km width and 50 m depth are visible both on the eastern and western shelf, but are limited to the

  9. Largemouth bass virus in Texas: distribution and management issues.

    PubMed

    Southard, Gregory M; Fries, Loraine T; Terre, David R

    2009-03-01

    In response to fish kills at prominent fishing sites for largemouth bass Micropterus salmoides, such as Lake Fork and Sam Rayburn Reservoir, the Texas Parks and Wildlife Department began a systematic evaluation of state waters for the presence of largemouth bass virus (LMBV). The survey comprised 49 water bodies and 13 river basins, and a total of 2,876 adult bass were collected by electrofishing and angling during the summer and fall of 2000. The virus was initially detected by means of cell culture and its presence subsequently confirmed by polymerase chain reaction. Fourteen reservoirs in eight river basins in eastern and central Texas tested positive for LMBV. Lake Fork was also tested to determine the prevalence of infection following a 1999 LMBV fish kill. The overall prevalence was low in all of the water bodies tested (1.50 +/- 2.82% [mean +/- SD]) as well as those determined to contain LMBV (5.00 +/- 3.02%). Largemouth bass testing positive for LMBV had a significantly higher prevalence of swim bladder anomalies, but this condition was not a good indicator of LMBV infection. No significant relationships were found between LMBV-positive fish and other factors investigated, including the presence or absence of grossly visible injury, hook marks, external parasites, known water quality problems, gender, allozyme-phenotype, method of capture, length, weight, body condition (relative weight), or age. This survey provided a means of gathering scientific information about LMBV, including its distribution in Texas. From the information gained by this survey, prior fish kills, and previous sampling efforts, a total of 19 water bodies within 9 of the 13 major river basins in the state were found to contain the virus. These results were used to guide a statewide fish stocking strategy aimed at preventing the spread of LMBV in Texas and to contribute to a nationwide effort to understand this virus and its effects on largemouth bass fisheries.

  10. Salting our freshwater lakes.

    PubMed

    Dugan, Hilary A; Bartlett, Sarah L; Burke, Samantha M; Doubek, Jonathan P; Krivak-Tetley, Flora E; Skaff, Nicholas K; Summers, Jamie C; Farrell, Kaitlin J; McCullough, Ian M; Morales-Williams, Ana M; Roberts, Derek C; Ouyang, Zutao; Scordo, Facundo; Hanson, Paul C; Weathers, Kathleen C

    2017-04-25

    The highest densities of lakes on Earth are in north temperate ecosystems, where increasing urbanization and associated chloride runoff can salinize freshwaters and threaten lake water quality and the many ecosystem services lakes provide. However, the extent to which lake salinity may be changing at broad spatial scales remains unknown, leading us to first identify spatial patterns and then investigate the drivers of these patterns. Significant decadal trends in lake salinization were identified using a dataset of long-term chloride concentrations from 371 North American lakes. Landscape and climate metrics calculated for each site demonstrated that impervious land cover was a strong predictor of chloride trends in Northeast and Midwest North American lakes. As little as 1% impervious land cover surrounding a lake increased the likelihood of long-term salinization. Considering that 27% of large lakes in the United States have >1% impervious land cover around their perimeters, the potential for steady and long-term salinization of these aquatic systems is high. This study predicts that many lakes will exceed the aquatic life threshold criterion for chronic chloride exposure (230 mg L -1 ), stipulated by the US Environmental Protection Agency (EPA), in the next 50 y if current trends continue.

  11. Morphometry and mixing regime of a tropical lake: Lake Nova (Southeastern Brazil).

    PubMed

    Gonçalves, Monica A; Garcia, Fábio C; Barroso, Gilberto F

    2016-09-01

    Lake Nova (15.5 km2) is the second largest lake in the Lower Doce River Valley (Southeastern Brazil). A better understanding of ecosystem structure and functioning requires knowledge about lake morphometry, given that lake basin form influences water column stratification. The present study aims to contribute to the understanding of relationship between morphometry and mixing patterns of deep tropical lakes in Brazil. Water column profiles of temperature and dissolved oxygen were taken on four sampling sites along the lake major axis during 2011, 2012 and 2013. The bathymetric survey was carried out in July 2011, along 131.7 km of hydrographic tracks yield 51,692 depth points. Morphometric features of lake size and form factors describe the relative deep subrectangular elongated basin with maximum length of 15.7 km, shoreline development index 5.0, volume of 0.23 km3, volume development of 1.3, and maximum, mean and relative depths of 33.9 m, 14.7 m and 0.7 %, respectively. The deep basin induces a monomictic pattern, with thermal stratification during the wet/warm season associated with anoxic bottom waters (1/3 of lake volume), and mixing during dry and cool season. Based on in situ measurements of tributary river discharges, theoretical retention time (RT) has been estimated in 13.4 years. The morphometry of Lake Nova promote long water RT and the warm monomictic mixing pattern, which is in accordance to the deep tropical lakes in Brazil.

  12. Characterization of Lake Michigan coastal lakes using zooplankton assemblages

    USGS Publications Warehouse

    Whitman, Richard L.; Nevers, Meredith B.; Goodrich, Maria L.; Murphy, Paul C.; Davis, Bruce M.

    2004-01-01

    Zooplankton assemblages and water quality were examined bi-weekly from 17 April to 19 October 1998 in 11 northeastern Lake Michigan coastal lakes of similar origin but varied in trophic status and limnological condition. All lakes were within or adjacent to Sleeping Bear Dunes National Lakeshore, Michigan. Zooplankton (principally microcrustaceans and rotifers) from triplicate Wisconsin net (80 I?m) vertical tows taken at each lake's deepest location were analyzed. Oxygen-temperature-pH-specific conductivity profiles and surface water quality were concurrently measured. Bray-Curtis similarity analysis showed small variations among sample replicates but large temporal differences. The potential use of zooplankton communities for environmental lake comparisons was evaluated by means of BIOENV (Primer 5.1) and principal component analyses. Zooplankton analyzed at the lowest identified taxonomic level yielded greatest sensitivity to limnological variation. Taxonomic and ecological aggregations of zooplankton data performed comparably, but less well than the finest taxonomic analysis. Secchi depth, chlorophyll a, and sulfate concentrations combined to give the best correlation with patterns of variation in the zooplankton data set. Principal component analysis of these variables revealed trophic status as the most influential major limnological gradient among the study lakes. Overall, zooplankton abundance was an excellent indicator of variation in trophic status.

  13. Lake Tahoe

    EPA Pesticide Factsheets

    Information on the Lake Tahoe watershed, EPA's protection efforts, water quality issues, effects of climate change, Lake Tahoe Total Maximum Daily Load (TMDL), EPA-sponsored projects, list of partner agencies.

  14. Geographical distributions of lake trout strains stocked in Lake Ontario

    USGS Publications Warehouse

    Elrod, Joseph H.; O'Gorman, Robert; Schneider, Clifford P.; Schaner, Ted

    1996-01-01

    Geographical distributions of lake trout (Salvelinus namaycush) stocked at seven locations in U.S. waters and at four locations in Canadian waters of Lake Ontario were determined from fish caught with gill nets in September in 17 areas of U.S. waters and at 10 fixed locations in Canadian waters in 1986-95. For fish of a given strain stocked at a given location, geographical distributions were not different for immature males and immature females or for mature males and mature females. The proportion of total catch at the three locations nearest the stocking location was higher for mature fish than for immature fish in all 24 available comparisons (sexes combined) and was greater for fish stocked as yearlings than for those stocked as fingerlings in all eight comparisons. Mature fish were relatively widely dispersed from stocking locations indicating that their tendency to return to stocking locations for spawning was weak, and there was no appreciable difference in this tendency among strains. Mature lake trout were uniformly distributed among sampling locations, and the strain composition at stocking locations generally reflected the stocking history 5 to 6 years earlier. Few lake trout moved across Lake Ontario between the north and south shores or between the eastern outlet basin and the main lake basin. Limited dispersal from stocking sites supports the concept of stocking different genetic strains in various parts of the lake with the attributes of each strain selected to match environmental conditions in the portion of the lake where it is stocked.

  15. Effect of a serogroup A meningococcal conjugate vaccine (PsA-TT) on serogroup A meningococcal meningitis and carriage in Chad: a community study [corrected].

    PubMed

    Daugla, D M; Gami, J P; Gamougam, K; Naibei, N; Mbainadji, L; Narbé, M; Toralta, J; Kodbesse, B; Ngadoua, C; Coldiron, M E; Fermon, F; Page, A-L; Djingarey, M H; Hugonnet, S; Harrison, O B; Rebbetts, L S; Tekletsion, Y; Watkins, E R; Hill, D; Caugant, D A; Chandramohan, D; Hassan-King, M; Manigart, O; Nascimento, M; Woukeu, A; Trotter, C; Stuart, J M; Maiden, McJ; Greenwood, B M

    2014-01-04

    A serogroup A meningococcal polysaccharide-tetanus toxoid conjugate vaccine (PsA-TT, MenAfriVac) was licensed in India in 2009, and pre-qualified by WHO in 2010, on the basis of its safety and immunogenicity. This vaccine is now being deployed across the African meningitis belt. We studied the effect of PsA-TT on meningococcal meningitis and carriage in Chad during a serogroup A meningococcal meningitis epidemic. We obtained data for the incidence of meningitis before and after vaccination from national records between January, 2009, and June, 2012. In 2012, surveillance was enhanced in regions where vaccination with PsA-TT had been undertaken in 2011, and in one district where a reactive vaccination campaign in response to an outbreak of meningitis was undertaken. Meningococcal carriage was studied in an age-stratified sample of residents aged 1-29 years of a rural area roughly 13-15 and 2-4 months before and 4-6 months after vaccination. Meningococci obtained from cerebrospinal fluid or oropharyngeal swabs were characterised by conventional microbiological and molecular methods. Roughly 1·8 million individuals aged 1-29 years received one dose of PsA-TT during a vaccination campaign in three regions of Chad in and around the capital N'Djamena during 10 days in December, 2011. The incidence of meningitis during the 2012 meningitis season in these three regions was 2·48 per 100,000 (57 cases in the 2·3 million population), whereas in regions without mass vaccination, incidence was 43·8 per 100,000 (3809 cases per 8·7 million population), a 94% difference in crude incidence (p<0·0001), and an incidence rate ratio of 0·096 (95% CI 0·046-0·198). Despite enhanced surveillance, no case of serogroup A meningococcal meningitis was reported in the three vaccinated regions. 32 serogroup A carriers were identified in 4278 age-stratified individuals (0·75%) living in a rural area near the capital 2-4 months before vaccination, whereas only one serogroup A

  16. Deglaciation, lake levels, and meltwater discharge in the Lake Michigan basin

    USGS Publications Warehouse

    Colman, Steven M.; Clark, J.A.; Clayton, L.; Hansel, A.K.; Larsen, C.E.

    1994-01-01

    The deglacial history of the Lake Michigan basin, including discharge and routing of meltwater, is complex because of the interaction among (1) glacial retreats and re-advances in the basin (2) the timing of occupation and the isostatic adjustment of lake outlets and (3) the depositional and erosional processes that left evidence of past lake levels. In the southern part of the basin, a restricted area little affected by differential isostasy, new studies of onshore and offshore areas allow refinement of a lake-level history that has evolved over 100 years. Important new data include the recognition of two periods of influx of meltwater from Lake Agassiz into the basin and details of the highstands gleaned from sedimentological evidence. Major disagreements still persist concerning the exact timing and lake-level changes associated with the Algonquin phase, approximately 11,000 BP. A wide variety of independent data suggests that the Lake Michigan Lobe was thin, unstable, and subject to rapid advances and retreats. Consequently, lake-level changes were commonly abrupt and stable shorelines were short-lived. The long-held beliefs that the southern part of the basin was stable and separated from deformed northern areas by a hinge-line discontinuity are becoming difficult to maintain. Numerical modeling of the ice-earth system and empirical modeling of shoreline deformation are both consistent with observed shoreline tilting in the north and with the amount and pattern of modern deformation shown by lake-level gauges. New studies of subaerial lacustrine features suggest the presence of deformed shorelines higher than those originally ascribed to the supposed horizontal Glenwood level. Finally, the Lake Michigan region as a whole appears to behave in a similar manner to other areas, both local (other Great Lakes) and regional (U.S. east coast), that have experienced major isostatic changes. Detailed sedimentological and dating studies of field sites and additional

  17. 77 FR 39638 - Safety Zone; Barbara Harder Wedding Fireworks, Lake Erie, Lake View, NY

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-07-05

    ...-AA00 Safety Zone; Barbara Harder Wedding Fireworks, Lake Erie, Lake View, NY AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary safety zone on Lake Erie, Lake View, NY. This safety zone is intended to restrict vessels from a portion of Lake Erie...

  18. Genetic diversity of lake whitefish in lakes Michigan and Huron: sampling, standardization, and research priorities

    USGS Publications Warehouse

    Stott, Wendylee; VanDeHey, Justin A.; Sloss, Brian L.

    2010-01-01

    We combined data from two laboratories to increase the spatial extent of a genetic data set for lake whitefish Coregonus clupeaformis from lakes Huron and Michigan and saw that genetic diversity was greatest between lakes, but that there was also structuring within lakes. Low diversity among stocks may be a reflection of relatively recent colonization of the Great Lakes, but other factors such as recent population fluctuation and localized stresses such as lamprey predation or heavy exploitation may also have a homogenizing effect. Our data suggested that there is asymmetrical movement of lake whitefish between Lake Huron and Lake Michigan; more genotypes associated with Lake Michigan were observed in Lake Huron. Adding additional collections to the calibrated set will allow further examination of diversity in other Great Lakes, answer questions regarding movement among lakes, and estimate contributions of stocks to commercial yields. As the picture of genetic diversity and population structure of lake whitefish in the Great Lakes region emerges, we need to develop methods to combine data types to help identify important areas for biodiversity and thus conservation. Adding genetic data to existing models will increase the precision of predictions of the impacts of new stresses and changes in existing pressures on an ecologically and commercially important species.

  19. [Characterizing chromophoric dissolved organic matter (CDOM) in Lake Honghu, Lake Donghu and Lake Liangzihu using excitation-emission matrices (EEMs) fluorescence and parallel factor analysis (PARAFAC)].

    PubMed

    Zhou, Yong-Qiang; Zhang, Yun-Lin; Niu, Cheng; Wang, Ming-Zhu

    2013-12-01

    Little is known about DOM characteristics in medium to large sized lakes located in the middle and lower reaches of Yangtze River, like Lake Honghu, Lake Donghu and Lake Liangzihu. Absorption, fluorescence and composition characteristics of chromophoric dissolved organic matter (CDOM) are presented using the absorption spectroscopy, the excitation-emission ma trices (EEMs) fluorescence and parallel factor analysis (PARAFAC) model based on the data collected in Sep-Oct. 2007 including 15, 9 and 10 samplings in Lake Honghu, Lake Donghu and Lake Liangzihu, respectively. CDOM absorption coefficient at 350 nm a(350) coefficient in Lake Honghu was significantly higher than those in Lake Donghu and Lake Liangzihu (t-test, p< 0. 001). A significant negative correlation was found between CDOM spectral slope in the wavelength range of 280-500 nm (S280-500) and a(350) (R2 =0. 781, p<0. 001). The mean value of S280-500 in Lake Honghu was significantly lower than those in Lake Donghu (t-test, pLake Liangzihu (t-test, p<0. 001). The mean value of spectral slope ratio SR in Lake Honghu was also significantly lower than those in Lake Donghu and Lake Liangzihu (t-test, p<0. 05). Two humic-like (C1, C2) and two protein-like fluorescent components (C3, C4) were identified by PARAFAC model, among which significant positive correlations were found between C1 and C2 (R2 =0. 884, p<0. 001), C3 and C4 (R2 =0. 677, p<0.001), respectively, suggesting that the sources of the two humic-like components as well as the two protein-like components were similar. However, no significant correlation has been found between those 4 fluorescent components and DOC concentration. Th e fluorescenceindices of FI255 (HIX), Fl265, FI310 (BIX) and Fl370 in Lake Donghu were all significantly higher than those in Lake Liangzihu (t-test, p <0.05) and Lake Honghu (t-test, p<0. 01), indicating that the eutrophication status in Lake Donghu was higher than Lake Honghu and Lake Liangzihu.

  20. Exploring trends, causes, and consequences of declining lipids in Lake Superior lake trout

    EPA Science Inventory

    The ability of lake trout to forage in deepwater habitats is facilitated by high lipid content, which affords buoyancy. In Lake Superior, lean lake trout historically occupied depths < 80 m, and siscowet lake trout occupied depths > 80 m. Siscowets have been known f...

  1. Great Lakes

    USGS Publications Warehouse

    Edsall, Thomas A.; Mac, Michael J.; Opler, Paul A.; Puckett Haecker, Catherine E.; Doran, Peter D.

    1998-01-01

    The Great Lakes region, as defined here, includes the Great Lakes and their drainage basins in Minnesota, Wisconsin, Illinois, Indiana, Ohio, Pennsylvania, and New York. The region also includes the portions of Minnesota, Wisconsin, and the 21 northernmost counties of Illinois that lie in the Mississippi River drainage basin, outside the floodplain of the river. The region spans about 9º of latitude and 20º of longitude and lies roughly halfway between the equator and the North Pole in a lowland corridor that extends from the Gulf of Mexico to the Arctic Ocean.The Great Lakes are the most prominent natural feature of the region (Fig. 1). They have a combined surface area of about 245,000 square kilometers and are among the largest, deepest lakes in the world. They are the largest single aggregation of fresh water on the planet (excluding the polar ice caps) and are the only glacial feature on Earth visible from the surface of the moon (The Nature Conservancy 1994a).The Great Lakes moderate the region’s climate, which presently ranges from subarctic in the north to humid continental warm in the south (Fig. 2), reflecting the movement of major weather masses from the north and south (U.S. Department of the Interior 1970; Eichenlaub 1979). The lakes act as heat sinks in summer and heat sources in winter and are major reservoirs that help humidify much of the region. They also create local precipitation belts in areas where air masses are pushed across the lakes by prevailing winds, pick up moisture from the lake surface, and then drop that moisture over land on the other side of the lake. The mean annual frost-free period—a general measure of the growing-season length for plants and some cold-blooded animals—varies from 60 days at higher elevations in the north to 160 days in lakeshore areas in the south. The climate influences the general distribution of wild plants and animals in the region and also influences the activities and distribution of the human

  2. Lakes, Lagerstaetten, and Evolution

    NASA Astrophysics Data System (ADS)

    Kordesch, E. G.; Park, L. E.

    2001-12-01

    The diversity of terrestrial systems is estimated to be greater than in the marine realm. However no hard data yet exists to substantiate this claim. Ancient lacustrine deposits may preserve an exceptionally diverse fossil fauna and aid in determining continental faunal diversities. Fossils preserved in lake deposits, especially those with exceptional preservation (i.e. Konservat Lagerstaetten), may represent a dependable method for determining species diversity changes in the terrestrial environment because of their faunal completeness. Important Konservat Lagerstaetten, such as the Green River Formation (US) and Messel (Germany), both Eocene in age, are found in lake sediments and show a remarkable faunal diversity for both vertebrates and invertebrates. To date information from nearly 25 lake lagerstaetten derived from different types of lake basins from the Carboniferous to the Miocene have been collected and described. Carboniferous sites derive from the cyclothems of Midcontinent of the US while many Cenozoic sites have been described from North and South America as well as Europe and Australia. Asian sites contain fossils from the Mesozoic and Cenozoic. With this data, insight into the evolutionary processes associated with lake systems can be examined. Do lakes act as unique evolutionary crucibles in contrast to marine systems? The speciation of cichlid fishes in present-day African lakes appears to be very high and is attributed to the diversity of environments found in large rift lakes. Is this true of all ancient lakes or just large rift lakes? The longevity of a lake system may be an important factor in allowing speciation and evolutionary processes to occur; marine systems are limited only in the existence of environments as controlled by tectonics and sea level changes, on the order of tens of millions of years. Rift lakes are normally the longest lived in the millions of years. Perhaps there are only certain types of lakes in which speciation of

  3. Suspended-sediment budget, flow distribution, and lake circulation for the Fox Chain of Lakes in Lake and McHenry Counties, Illinois, 1997-99

    USGS Publications Warehouse

    Schrader, David L.; Holmes, Robert R.

    2000-01-01

    The Fox Chain of Lakes is a glacial lake system in McHenry and Lake Counties in northern Illinois and southern Wisconsin. Sedimentation and nutrient overloading have occurred in the lake system since the first dam was built (1907) in McHenry to raise water levels in the lake system. Using data collected from December 1, 1997, to June 1, 1999, suspended-sediment budgets were constructed for the most upstream lake in the system, Grass Lake, and for the lakes downstream from Grass Lake. A total of 64,900 tons of suspended sediment entered Grass Lake during the study, whereas a total of 70,600 tons of suspended sediment exited the lake, indicating a net scour of 5,700 tons of sediment. A total of 44,100 tons of suspended sediment was measured exiting the Fox Chain of Lakes at Johnsburg, whereas 85,600 tons entered the system downstream from Grass Lake. These suspended-sediment loads indicate a net deposition of 41,500 tons downstream from Grass Lake, which represents a trapping efficiency of 48.5 percent. A large amount of recreational boating takes place on the Fox Chain of Lakes during summer months, and suspended-sediment load was observed to rise from 110 tons per day to 339 tons per day during the 1999 Memorial Day weekend (May 26 ?31, 1999). Presumably, this rise was the result of the boating traffic because no other hydrologic event is known to have occurred that might have caused the rise. This study covers a relatively short period and may not represent the long-term processes of the Fox Chain of Lakes system, although the sediment transport was probably higher than an average year. The bed sediments found on the bottom of the lakes are composed of mainly fine particles in the silt-clay range. The Grass Lake sediments were characterized as black peat with an organic content of between 9 and 18 percent, and the median particle size ranged from 0.000811 to 0.0013976 inches. Other bed material samples were collected at streamflow-gaging stations on the

  4. Changing abundance of Hexagenia mayfly nymphs in western Lake Erie of the Laurentian Great Lakes: Impediments to assessment of lake recovery?

    USGS Publications Warehouse

    Schloesser, D.W.; Nalepa, T.F.

    2001-01-01

    After an absence of 40 years, mayfly nymphs of the genus Hexagenia were found in sediments of western Lake Erie of the Laurentian Great Lakes in 1993 and, by 1997, were abundant enough to meet a mayfly-density management goal (ca. 350 nymphs m—2) based on pollution-abatement programs. We sampled nymphs in western Lake Erie and Lake St. Clair, located upstream of western Lake Erie, to determine the importance of seasonal abundance and life-history characteristics of nymphs (e.g., emergence and recruitment) on density estimates relative to the mayfly-density management goal. Two types of density patterns were observed: (1) densities were relatively high in spring and gradually decreased through late summer (observed in Lake Erie and Lake St. Clair in 1997 and Lake St. Clair in 1999) and (2) densities were relatively high in spring, gradually decreased to mid summer, abruptly decreased in mid summer, and then increased between summer and late fall (Lake Erie and Lake St. Clair in 1998 and Lake Erie in 1999). Length-frequency distributions of nymphs and observations of adults indicate that the primary cause for the two density patterns was attributed to failed (first pattern) and successful (second pattern) reproduction and emergence of nymphs into adults in mid summer. Gradual declines in densities were attributed to mortality of nymphs. Our results indicate that caution should be used when evaluating progress of pollution-abatement programs based on mayfly densities because recruitment success is variable both between and within years. Additionally, the interpretation of progress toward management goals, relative to the restoration of Hexagenia populations in the Great Lakes and possibly other water bodies throughout the world, is influenced by the number of years in which consequtive collections are made.

  5. 33 CFR 162.220 - Hoover Dam, Lake Mead, and Lake Mohave (Colorado River), Ariz.-Nev.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Hoover Dam, Lake Mead, and Lake... REGULATIONS § 162.220 Hoover Dam, Lake Mead, and Lake Mohave (Colorado River), Ariz.-Nev. (a) Lake Mead and... the axis of Hoover Dam and that portion of Lake Mohave (Colorado River) extending 4,500 feet...

  6. 33 CFR 162.220 - Hoover Dam, Lake Mead, and Lake Mohave (Colorado River), Ariz.-Nev.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Hoover Dam, Lake Mead, and Lake... REGULATIONS § 162.220 Hoover Dam, Lake Mead, and Lake Mohave (Colorado River), Ariz.-Nev. (a) Lake Mead and... the axis of Hoover Dam and that portion of Lake Mohave (Colorado River) extending 4,500 feet...

  7. 33 CFR 162.220 - Hoover Dam, Lake Mead, and Lake Mohave (Colorado River), Ariz.-Nev.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Hoover Dam, Lake Mead, and Lake... REGULATIONS § 162.220 Hoover Dam, Lake Mead, and Lake Mohave (Colorado River), Ariz.-Nev. (a) Lake Mead and... the axis of Hoover Dam and that portion of Lake Mohave (Colorado River) extending 4,500 feet...

  8. 33 CFR 162.220 - Hoover Dam, Lake Mead, and Lake Mohave (Colorado River), Ariz.-Nev.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Hoover Dam, Lake Mead, and Lake... REGULATIONS § 162.220 Hoover Dam, Lake Mead, and Lake Mohave (Colorado River), Ariz.-Nev. (a) Lake Mead and... the axis of Hoover Dam and that portion of Lake Mohave (Colorado River) extending 4,500 feet...

  9. Lake Characteristics Influencing Spawning Success of Muskellunge in Northern Wisconsin Lakes

    Treesearch

    Ashley J. Rust; James S. Diana; Terry L. Margenau; Clayton J. Edwards

    2002-01-01

    We determined the physical, chemical, biological, and land use characteristics that distinguish northern Wisconsin lakes with self-sustaining populations of muskellunge Esox masquinongy from lakes where stocking is required to maintain populations. Lakes that supported self-sustaining muskellunge populations were characterized by fewer shoreline...

  10. Evaluating COSMO's lake module (FLake) for an East-African lake using a comprehensive set of lake temperature profiles

    NASA Astrophysics Data System (ADS)

    Thiery, W.; Martynov, A.; Darchambeau, F.; Demuzere, M.; van Lipzig, N.

    2012-04-01

    The African great lakes are of utmost importance for the local economy (fishing), as well as being essential to the survival of the local people. During last decades, these lakes have been changing rapidly and their evolution is a major concern. Hence, it is important to correctly represent them in regional climate models for simulations over tropical Africa. However, so far lake models have been developed and tested primarily for boreal conditions. In this study, for the first time the freshwater lake model FLake is evaluated over East-Africa, more specifically over lake Kivu. Meteorological observations from January 2003 to December 2008 from an automatic weather station in Bukavu, DRC, are used to drive the standalone version of FLake. For the evaluation, a unique dataset is used which contains over 200 temperature profiles recorded since 2002. Results show that FLake in its default configuration is very successful at reproducing both the timing and magnitude of the seasonal cycle at 5 m depth. Flake captures that this seasonality is regulated by the water vapour pressure, which constrains evaporation except during summer (JJA). A positive bias of ~1 K is attributed to the driving data, which are collected in the city and are therefore expected to mirror higher temperatures and lower wind speeds compared to the lake surface. The evaluation also showed that driving FLake with Era-Interim from the nearest pixel does only slightly deteriorate the model performance. Using forcing fields from the Canadian Regional Climate Model, version 5 (CRCM5) simulation output gives similar performance as Era-Interim. Furthermore, a drawback of FLake is that it does not account for salinity and its effect upon lake stratification, and therefore requires artificial initial conditions for both lake depth and bottom temperature in order to reproduce the correct mixing regime in lake Kivu. Further research will therefore aim at improving FLake's representation of tropical lakes.

  11. Seasonal thermal ecology of adult walleye (Sander vitreus) in Lake Huron and Lake Erie.

    PubMed

    Peat, Tyler B; Hayden, Todd A; Gutowsky, Lee F G; Vandergoot, Christopher S; Fielder, David G; Madenjian, Charles P; Murchie, Karen J; Dettmers, John M; Krueger, Charles C; Cooke, Steven J

    2015-10-01

    The purpose of this study was to characterize thermal patterns and generate occupancy models for adult walleye from lakes Erie and Huron with internally implanted biologgers coupled with a telemetry study to assess the effects of sex, fish size, diel periods, and lake. Sex, size, and diel periods had no effect on thermal occupancy of adult walleye in either lake. Thermal occupancy differed between lakes and seasons. Walleye from Lake Erie generally experienced higher temperatures throughout the spring and summer months than did walleye in Lake Huron, due to limnological differences between the lakes. Tagged walleye that remained in Saginaw Bay, Lake Huron (i.e., adjacent to the release location), as opposed to those migrating to the main basin of Lake Huron, experienced higher temperatures, and thus accumulated more thermal units (the amount of temperature units amassed over time) throughout the year. Walleye that migrated toward the southern end of Lake Huron occupied higher temperatures than those that moved toward the north. Consequently, walleye that emigrated from Saginaw Bay experienced thermal environments that were more favorable for growth as they spent more time within their thermal optimas than those that remained in Saginaw Bay. Results presented in this paper provide information on the thermal experience of wild fish in a large lake, and could be used to refine sex- and lake-specific bioenergetics models of walleye in the Great Lakes to enable the testing of ecological hypotheses. Copyright © 2015 Elsevier Ltd. All rights reserved.

  12. Seasonal thermal ecology of adult walleye (Sander vitreus) in Lake Huron and Lake Erie

    USGS Publications Warehouse

    Peat, Tyler B; Hayden, Todd A.; Gutowsky, Lee F G; Vandergoot, Christopher S.; Fielder, David G.; Madenjian, Charles P.; Murchie, Karen J; Dettmers, John M.; Krueger, Charles C.; Cooke, Steven J.

    2015-01-01

    The purpose of this study was to characterize thermal patterns and generate occupancy models for adult walleye from lakes Erie and Huron with internally implanted biologgers coupled with a telemetry study to assess the effects of sex, fish size, diel periods, and lake. Sex, size, and diel periods had no effect on thermal occupancy of adult walleye in either lake. Thermal occupancy differed between lakes and seasons. Walleye from Lake Erie generally experienced higher temperatures throughout the spring and summer months than did walleye in Lake Huron, due to limnological differences between the lakes. Tagged walleye that remained in Saginaw Bay, Lake Huron (i.e., adjacent to the release location), as opposed to those migrating to the main basin of Lake Huron, experienced higher temperatures, and thus accumulated more thermal units (the amount of temperature units amassed over time) throughout the year. Walleye that migrated toward the southern end of Lake Huron occupied higher temperatures than those that moved toward the north. Consequently, walleye that emigrated from Saginaw Bay experienced thermal environments that were more favorable for growth as they spent more time within their thermal optimas than those that remained in Saginaw Bay. Results presented in this paper provide information on the thermal experience of wild fish in a large lake, and could be used to refine sex- and lake-specific bioenergetics models of walleye in the Great Lakes to enable the testing of ecological hypotheses.

  13. [Evaluation of Knowledge-Attitudes-Practices of the populations in the health districts of Benoye, Laoukassy, Moundou and South N'Djamena towards canine rabies in Chad].

    PubMed

    Mindekem, Rolande; Lechenne, Monique; Alfaroukh, Idriss Oumar; Moto, Daugla Doumagoum; Zinsstag, Jakob; Ouedraogo, Laurent Tinoaga; Salifou, Sahidou

    2017-01-01

    Canine rabies remains a concern in Africa as well as in Chad. Our study aimed to evaluate the knowledge, attitudes and practices of the populations towards the appropriate management of people exposed to canine rabies and effective fight against it. We conducted a cross-sectional, descriptive study in four health districts in Chad in July and in September 2015. Data were collected from households recruited by three-stage random sampling by means of a questionnaire. We conducted a survey of 2428 individuals having completed at least primary education level (54,12%). The average age was 36 ± 13.50 years. Surveyed individuals were farmers (35,17%), merchants (18,04%), households (12.81%). Rabies was defined as a disease transmitted from the dog to the man (41.43%), an alteration in brain function (41.27%), an undernourishment (10.26%). The cat was little-known to be a reservoir(13.84%) and a vector (19,77%) as well as licking was little-known to be a transmission medium (4.61%) and cat vaccination to be a preventive measure (0.49%). First aid for a bite at home was the traditional practice (47,69%), wounds washing (19.48%) or no action undertaken (20.43%). Households consulted the Health Service (78.50%), the Animal Health Service (5.35%) and the traditional healers (27%). A communication campaing for implementing first aid at home in the event of a bite, knowledge about the cat as a reservoir and a vector and licking as a transmission medium as well as the promotion of the consultation of veterinary services in the event of a bite are necessary.

  14. Hydrology of Indiana lakes

    USGS Publications Warehouse

    Perrey, Joseph Irving; Corbett, Don Melvin

    1956-01-01

    The stabilization of lake levels often requires the construction of outlet control structures. A detailed study of past lake-level elevations and other hydologic date is necessary to establish a level that can be maintained and to determine the means necessary for maintaining the established level. Detailed lake-level records for 28 lakes are included in the report, and records for over 100 other lakes data are available in the U.S. Geological Survey Office, Indianapolis, Ind. Evaporation data from the four Class A evaporation station of the U. S. Weather Bureau have been compiled in this report. A table showing the established legal lake level and related data is included.

  15. First evidence of successful natural reproduction by planted lake trout in Lake Huron

    USGS Publications Warehouse

    Nester, Robert T.; Poe, Thomas P.

    1984-01-01

    Twenty-two lake trout (Salvelinus namaycush) swim-up fry, 24-27 mm long, were captured with emergent fry traps and a tow net in northwestern Lake Huron on a small nearshore reef off Alpena, Michigan, between May 10 and June 1, 1982. These catches represent the first evidence of successful production of swim-up fry by planted, hatchery-reared lake trout in Lake Huron since the lake trout rehabilitation program began in 1973.

  16. Estimation of lake water - groundwater interactions in meromictic mining lakes by modelling isotope signatures of lake water.

    PubMed

    Seebach, Anne; Dietz, Severine; Lessmann, Dieter; Knoeller, Kay

    2008-03-01

    A method is presented to assess lake water-groundwater interactions by modelling isotope signatures of lake water using meteorological parameters and field data. The modelling of delta(18)O and deltaD variations offers information about the groundwater influx into a meromictic Lusatian mining lake. Therefore, a water balance model is combined with an isotope water balance model to estimate analogies between simulated and measured isotope signatures within the lake water body. The model is operated with different evaporation rates to predict delta(18)O and deltaD values in a lake that is only controlled by weather conditions with neither groundwater inflow nor outflow. Comparisons between modelled and measured isotope values show whether the lake is fed by the groundwater or not. Furthermore, our investigations show that an adaptation of the Craig and Gordon model [H. Craig, L.I. Gordon. Deuterium and oxygen-18 variations in the ocean and the marine atmosphere. In Stable Isotopes in Oceanographic Studies and Paleotemperature, Spoleto, E. Tongiorgi (Ed.), pp. 9-130, Consiglio Nazionale delle Ricerche, Laboratorio di Geologia Nucleare, Pisa (1965).] to specific conditions in temperate regions seems necessary.

  17. 75 FR 13232 - Safety Zone; Lake Mead Intake Construction, Lake Mead, Boulder City, NV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-19

    ...-AA00 Safety Zone; Lake Mead Intake Construction, Lake Mead, Boulder City, NV AGENCY: Coast Guard, DHS... waters of Lake Mead in support of the construction project for Lake Mead's Intake 3. This safety zone is... for the placement of an Intake Pipe from Lake Mead throughout 2010. This safety zone is necessary to...

  18. 78 FR 17869 - Safety Zone; Desert Storm Shootout; Lake Havasu, Lake Havasu City, AZ

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-25

    ...-AA00 Safety Zone; Desert Storm Shootout; Lake Havasu, Lake Havasu City, AZ AGENCY: Coast Guard, DHS... navigable waters of the Colorado River in Lake Havasu, Lake Havasu City, Arizona in support of the Desert... Coast Guard to establish safety zones (33 U.S.C 1221 et seq.). Lake Racer LLC is sponsoring the Desert...

  19. Bioturbation enhances the aerobic respiration of lake sediments in warming lakes.

    PubMed

    Baranov, Viktor; Lewandowski, Jörg; Krause, Stefan

    2016-08-01

    While lakes occupy less than 2% of the total surface of the Earth, they play a substantial role in global biogeochemical cycles. For instance, shallow lakes are important sites of carbon metabolism. Aerobic respiration is one of the important drivers of the carbon metabolism in lakes. In this context, bioturbation impacts of benthic animals (biological reworking of sediment matrix and ventilation of the sediment) on sediment aerobic respiration have previously been underestimated. Biological activity is likely to change over the course of a year due to seasonal changes of water temperatures. This study uses microcosm experiments to investigate how the impact of bioturbation (by Diptera, Chironomidae larvae) on lake sediment respiration changes when temperatures increase. While at 5°C, respiration in sediments with and without chironomids did not differ, at 30°C sediment respiration in microcosms with 2000 chironomids per m(2) was 4.9 times higher than in uninhabited sediments. Our results indicate that lake water temperature increases could significantly enhance lake sediment respiration, which allows us to better understand seasonal changes in lake respiration and carbon metabolism as well as the potential impacts of global warming. © 2016 The Authors.

  20. Bioturbation enhances the aerobic respiration of lake sediments in warming lakes

    PubMed Central

    Krause, Stefan

    2016-01-01

    While lakes occupy less than 2% of the total surface of the Earth, they play a substantial role in global biogeochemical cycles. For instance, shallow lakes are important sites of carbon metabolism. Aerobic respiration is one of the important drivers of the carbon metabolism in lakes. In this context, bioturbation impacts of benthic animals (biological reworking of sediment matrix and ventilation of the sediment) on sediment aerobic respiration have previously been underestimated. Biological activity is likely to change over the course of a year due to seasonal changes of water temperatures. This study uses microcosm experiments to investigate how the impact of bioturbation (by Diptera, Chironomidae larvae) on lake sediment respiration changes when temperatures increase. While at 5°C, respiration in sediments with and without chironomids did not differ, at 30°C sediment respiration in microcosms with 2000 chironomids per m2 was 4.9 times higher than in uninhabited sediments. Our results indicate that lake water temperature increases could significantly enhance lake sediment respiration, which allows us to better understand seasonal changes in lake respiration and carbon metabolism as well as the potential impacts of global warming. PMID:27484649

  1. Lake Sturgeon, Acipenser fulvescens, movements in Rainy Lake, Minnesota and Ontario

    USGS Publications Warehouse

    Adams, W.E.; Kallemeyn, L.W.; Willis, D.W.

    2006-01-01

    Rainy Lake, Minnesota-Ontario, contains a native population of Lake Sturgeon (Acipenser fulvescens) that has gone largely unstudied. The objective of this descriptive study was to summarize generalized Lake Sturgeon movement patterns through the use of biotelemetry. Telemetry data reinforced the high utilization of the Squirrel Falls geographic location by Lake Sturgeon, with 37% of the re-locations occurring in that area. Other spring aggregations occurred in areas associated with Kettle Falls, the Pipestone River, and the Rat River, which could indicate spawning activity. Movement of Lake Sturgeon between the Seine River and the South Arm of Rainy Lake indicates the likelihood of one integrated population on the east end of the South Arm. The lack of re-locations in the Seine River during the months of September and October may have been due to Lake Sturgeon moving into deeper water areas of the Seine River and out of the range of radio telemetry gear or simply moving back into the South Arm. Due to the movements between Minnesota and Ontario, coordination of management efforts among provincial, state, and federal agencies will be important.

  2. Stratigraphic framework and lake level history of Lake Kivu, East African Rift

    NASA Astrophysics Data System (ADS)

    Wood, Douglas A.; Scholz, Christopher A.

    2017-10-01

    Sediment cores and seismic reflection data acquired from the eastern basin of Lake Kivu, Rwanda reveal extensive limnologic variations due to changes in regional climate and basin structure. The eastern basin of the lake contains a sedimentary wedge which is > 1.5 km in thickness on its western side, and basal sediments are estimated to be at least 1.5 million years old. Sediments are likely to be thicker and older than this in the northern, Congolese basin of the lake. Above the ∼300 m iosbath only a thin layer of Holocene sediments are observed indication that this may have been the lake's high stand prior to that time. There are at least three erosional unconformities interpreted as desiccation or near-desiccation events which are estimated to have occurred at ∼475 ka, ∼100 ka, and ∼20 ka; the two most recent of these low stages likely developed during the African Megadrought and Last Glacial Maximum (LGM) periods. Following the LGM, the water levels rose to form a ∼100 m deep lake with its surface ∼370 m below the current lake level. The lake remained near that level for several thousand years and during this time the Virunga Volcanic Province expanded. At ∼12.2 ka a change to wetter climate conditions rapidly filled the lake to spill out of the Bukavu Bay basin southward toward Lake Tanganyika. Tephra sampled from the cores show that there have been at least 24 large local volcanic events since the early Holocene lake transgression.

  3. Plant viruses in aqueous environment - survival, water mediated transmission and detection.

    PubMed

    Mehle, Nataša; Ravnikar, Maja

    2012-10-15

    The presence of plant viruses outside their plant host or insect vectors has not been studied intensively. This is due, in part, to the lack of effective detection methods that would enable their detection in difficult matrixes and in low titres, and support the search for unknown viruses. Recently, new and sensitive methods for detecting viruses have resulted in a deeper insight into plant virus movement through, and transmission between, plants. In this review, we have focused on plant viruses found in environmental waters and their detection. Infectious plant pathogenic viruses from at least 7 different genera have been found in aqueous environment. The majority of the plant pathogenic viruses so far recovered from environmental waters are very stable, they can infect plants via the roots without the aid of a vector and often have a wide host range. The release of such viruses from plants can lead to their dissemination in streams, lakes, and rivers, thereby ensuring the long-distance spread of viruses that otherwise, under natural conditions, would remain restricted to limited areas. The possible sources and survival of plant viruses in waters are therefore discussed. Due to the widespread use of hydroponic systems and intensive irrigation in horticulture, the review is focused on the possibility and importance of spreading viral infection by water, together with measures for preventing the spread of viruses. The development of new methods for detecting multiple plant viruses at the same time, like microarrays or new generation sequencing, will facilitate the monitoring of environmental waters and waters used for irrigation and in hydroponic systems. It is reasonable to expect that the list of plant viruses found in waters will thereby be expanded considerably. This will emphasize the need for further studies to determine the biological significance of water-mediated transport. Copyright © 2012 Elsevier Ltd. All rights reserved.

  4. Hydrology of Crater, East and Davis Lakes, Oregon; with section on Chemistry of the Lakes

    USGS Publications Warehouse

    Phillips, Kenneth N.; Van Denburgh, A.S.

    1968-01-01

    Crater, East, and Davis Lakes are small bodies of fresh water that occupy topographically closed basins in Holocene volcanic terrane. Because the annual water supply exceeds annual evaporation, water must be lost by seepage from each lake. The seepage rates vary widely both in volume and in percentage of the total water supply. Crater Lake loses about 89 cfs (cubic feet per second), equivalent to about 72 percent of its average annual supply. East Lake loses about 2.3 cfs, or about 44 percent of its estimated supply. Davis Lake seepage varies greatly with lake level, but the average loss is about 150 cfs, more than 90 percent of its total supply. The destination of the seepage loss is not definitely known for any of the lakes. An approximate water budget was computed for stationary level for each lake, by using estimates 'by the writer to supplement the hydrologic data available. The three lake waters are dilute. Crater Lake contains about 80 ppm, (parts per million) of dissolved solids---mostly silica, sodium, and bicarbonate, and lesser amounts of calcium, sulfate, and chloride. Much of the dissolved-solids content of Crater Lake---especially the sulfate and chloride---may be related to fumarole and thermal-spring activity that presumably followed the collapse of Mount Mazama. Although Grater Lake loses an estimated 7,000 tons of its 1.5million-ton salt content each year by leakage, the chemical character of the lake did not change appreciably between 1912 and 1964. East Lake contains 200 ppm of dissolved solids, which includes major proportions of calcium, sodium, bicarbonate, and sulfate, but almost no chloride. The lake apparently receives much of its dissolved solids from subsurface thermal springs. Annual solute loss from East Lake by leakage is about 450 tons, or 3 percent of the lake's 15,000-ton estimated solute content. Davis Lake contains only 48 ppm of dissolved solids, much of which is silica and bicarbonate; chloride is almost completely absent

  5. 76 FR 2579 - Safety Zone; Lake Mead Intake Construction, Lake Mead, Boulder City, NV

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-14

    ...-AA00 Safety Zone; Lake Mead Intake Construction, Lake Mead, Boulder City, NV AGENCY: Coast Guard, DHS... waters of Lake Mead in support of the construction project for Lake Mead's Intake 3 during the first 6... blasting operations for the placement of a water intake pipe in Lake Mead during the first 6 months of 2011...

  6. Palaeoenvironments and palaeotectonics of the arid to hyperarid intracontinental latest Permian- late Triassic Solway basin (U.K.)

    NASA Astrophysics Data System (ADS)

    Brookfield, Michael E.

    2008-10-01

    The late Permian to late Triassic sediments of the Solway Basin consist of an originally flat-lying, laterally persistent and consistent succession of mature, dominantly fine-grained red clastics laid down in part of a very large intracontinental basin. The complete absence of body or trace fossils or palaeosols indicates a very arid (hyperarid) depositional environment for most of the sediments. At the base of the succession, thin regolith breccias and sandstones rest unconformably on basement and early Permian rift clastics. Overlying gypsiferous red silty mudstones, very fine sandstones and thick gypsum were deposited in either a playa lake or in a hypersaline estuary, and their margins. These pass upwards into thick-bedded, multi-storied, fine- to very fine-grained red quartzo-felspathic and sublithic arenites in which even medium sand is rare despite channels with clay pebbles up to 30 cm in diameter. Above, thick trough cross-bedded and parallel laminated fine-grained aeolian sandstones (deposited in extensive barchanoid dune complexes) pass up into very thick, multicoloured mudstones, and gypsum deposited in marginal marine or lacustrine sabkha environments. The latter pass up into marine Lower Jurassic shales and limestones. Thirteen non-marine clastic lithofacies are arranged into five main lithofacies associations whose facies architecture is reconstructed where possible by analysis of large exposures. The five associations can be compared with the desert pavement, arid ephemeral stream, sabkha, saline lake and aeolian sand dune environments of the arid to hyperarid areas of existing intracontinental basins such as Lake Eyre and Lake Chad. The accommodation space in such basins is controlled by gradual tectonic subsidence moderated by large fluctuations in shallow lake extent (caused by climatic change and local variation) and this promotes a large-scale layer-cake stratigraphy as exemplified in the Solway basin. Here, the dominant fine-grained mature

  7. Evaluation of methods to estimate lake herring spawner abundance in Lake Superior

    USGS Publications Warehouse

    Yule, D.L.; Stockwell, J.D.; Cholwek, G.A.; Evrard, L.M.; Schram, S.; Seider, M.; Symbal, M.

    2006-01-01

    Historically, commercial fishers harvested Lake Superior lake herring Coregonus artedi for their flesh, but recently operators have targeted lake herring for roe. Because no surveys have estimated spawning female abundance, direct estimates of fishing mortality are lacking. The primary objective of this study was to determine the feasibility of using acoustic techniques in combination with midwater trawling to estimate spawning female lake herring densities in a Lake Superior statistical grid (i.e., a 10′ latitude × 10′ longitude area over which annual commercial harvest statistics are compiled). Midwater trawling showed that mature female lake herring were largely pelagic during the night in late November, accounting for 94.5% of all fish caught exceeding 250 mm total length. When calculating acoustic estimates of mature female lake herring, we excluded backscattering from smaller pelagic fishes like immature lake herring and rainbow smelt Osmerus mordax by applying an empirically derived threshold of −35.6 dB. We estimated the average density of mature females in statistical grid 1409 at 13.3 fish/ha and the total number of spawning females at 227,600 (95% confidence interval = 172,500–282,700). Using information on mature female densities, size structure, and fecundity, we estimate that females deposited 3.027 billion (109) eggs in grid 1409 (95% confidence interval = 2.356–3.778 billion). The relative estimation error of the mature female density estimate derived using a geostatistical model—based approach was low (12.3%), suggesting that the employed method was robust. Fishing mortality rates of all mature females and their eggs were estimated at 2.3% and 3.8%, respectively. The techniques described for enumerating spawning female lake herring could be used to develop a more accurate stock–recruitment model for Lake Superior lake herring.

  8. Water-quality and lake-stage data for Wisconsin lakes, water year 2014

    USGS Publications Warehouse

    Manteufel, S. Bridgett; Robertson, Dale M.

    2017-05-25

    IntroductionThe U.S. Geological Survey (USGS), in cooperation with local and other agencies, collects data at selected lakes throughout Wisconsin. These data, accumulated over many years, provide a database for developing an improved understanding of the water quality of lakes. To make these data available to interested parties outside the USGS, the data are published annually in this report series. The locations of water-quality and lake-stage stations in Wisconsin for water year 2014 are shown in figure 1. A water year is the 12-month period from October 1 through September 30. It is designated by the calendar year in which it ends. Thus, the periodOctober 1, 2013, through September 30, 2014, is called “water year 2014.”The purpose of this report is to provide information about the chemical and physical characteristics of Wisconsin lakes. Data that have been collected at specific lakes, and information to aid in the interpretation of those data, are included in this report. Data collected include measurements of in-lake water quality and lake stage. Time series of Secchi depths, surface total phosphorus, and chlorophyll a concentrations collected during nonfrozen periods are included for many lakes. Graphs of vertical profiles of temperature, dissolved oxygen, pH, and specific conductance are included for sites where these parameters were measured. Descriptive information for each lake includes the location of the lake, area of the lake’s watershed, period for which data are available, revisions to previously published records, and pertinent remarks. Additional data, such as streamflow and water quality in tributary and outlet streams of some of the lakes, are published online at http://nwis.waterdata.usgs.gov/wi/nwis.Water-resources data, including stage and discharge data at most streamflow-gaging stations, are available online. The Wisconsin Water Science Center’s home page is at https://www.usgs.gov/centers/wisconsin-water-science-center. Information

  9. Helminths in an intensively stocked population of lake trout, Salvelinus namaycush, from Lake Huron

    USGS Publications Warehouse

    Muzzall, Patrick M.; Bowen, Charles A.

    2000-01-01

    Eighty stocked lake trout Salvelinus namaycush (Salmonidae), collected from 2 locations in Lake Huron in May 1995, were examined for parasites. The parasite fauna of this top predator in Lake Huron was characterized by only 6 helminth species. Echinorhynchus salmonis infected all lake trout with a mean intensity of 163.9. The intensity of this acanthocephalan species significantly increased with host length and weight. Eubothrium salvelini infected 78 lake trout with a maximum number of 81 scoleces counted. Diplostomum sp., Cyathocephalus truncatus, Capillaria salvelini, and Neoechinorhynchus sp. infrequently infected lake trout. The low parasite species richness in these lake trout is believed to be due to their large size at stocking and to the loss of historical enzootic host-parasite relationships that followed the absence of this fish species in Lake Huron for 26 yr.

  10. Glaciers, Glacial lakes and Glacial Lake Outburst Floods in the Koshi Basin

    NASA Astrophysics Data System (ADS)

    Shrestha, F.; Gao, X.; Khanal, N. R.; Maharjan, S. B.; Bajracharya, S. R.; Shrestha, R. B.; Lizong, W.; Mool, P. K.

    2016-12-01

    Glacier is a vital water resources for mountain communities. Recession in glacier area either increased the glacial lake size or develop a new lake. The consequences of these changes in lake has become one of the major issue in the management of GLOF risk. This paper presents the distribution of, and changes in, glaciers, glacial lakes in the Koshi basin and also looks at past GLOF events that have occurred in the basin and their distance of impact. Data on the number of glaciers and glacial lakes and their areas were generated for the years 1977, 1990, 2000, and 2010 using Landsat images. The study revealed that there were a total of 845 glaciers (Nepal side) and 2,168 glacial lakes (Nepal and China side) with a total area of 1,103 km2 and 127.608 km2 in 2010. The number of glacier increased by 15% (109) and area decreased by 26% (396 km2) over 33 years. In case of glacier lakes, the number and area increased from 1,160 to 2,168 and from 94.444 km2 to 127.608 km2 during 33 years with an overall growth rates of 86.9% and 35.1%. A large number of glacial lakes are small in size (≤ 0.1 km2). End moraine dammed lakes with area ≥ 0.1 km2 were selected to analyse the change characteristics of glacial lakes. The results show that there were 134 lakes ≥ 0.1 km2 in 2010; these lakes had a total area of 43.06 km2 in 1997, increased to 64.35 km2 in 2010. The distribution of lakes on the north side of the Himalayas (in China) was three times higher than on the south side of the Himalayas (in Nepal). Comparing the mean growth rate in area and length for the 33 years, the growth rate on the north side was found to be a little slower than that on the south side. This relationship did not hold true for length change in the different periods. The study identified 42 rapidly growing large lakes that are dangerous in terms of GLOF risk. In the past, 18 GLOF events have been reported. The downstream distance impacted by those events was up to 90 km. Among them, 13 GLOF events

  11. Lake Michigan lake trout PCB model forecast post audit (oral presentation)

    EPA Science Inventory

    Scenario forecasts for total PCBs in Lake Michigan (LM) lake trout were conducted using the linked LM2-Toxics and LM Food Chain models, supported by a suite of additional LM models. Efforts were conducted under the Lake Michigan Mass Balance Study and the post audit represents an...

  12. Simulating Lake-Groundwater Interactions During Decadal Climate Cycles: Accounting For Variable Lake Area In The Watershed

    NASA Astrophysics Data System (ADS)

    Virdi, M. L.; Lee, T. M.

    2009-12-01

    The volume and extent of a lake within the topo-bathymetry of a watershed can change substantially during wetter and drier climate cycles, altering the interaction of the lake with the groundwater flow system. Lake Starr and other seepage lakes in the permeable sandhills of central Florida are vulnerable to climate changes as they rely exclusively on rainfall and groundwater for inflows in a setting where annual rainfall and recharge vary widely. The groundwater inflow typically arrives from a small catchment area bordering the lake. The sinkhole origin of these lakes combined with groundwater pumping from underlying aquifers further complicate groundwater interactions. Understanding the lake-groundwater interactions and their effects on lake stage over multi-decadal climate cycles is needed to manage groundwater pumping and public expectation about future lake levels. The interdependence between climate, recharge, changing lake area and the groundwater catchment pose unique challenges to simulating lake-groundwater interactions. During the 10-year study period, Lake Starr stage fluctuated more than 13 feet and the lake surface area receded and expanded from 96 acres to 148 acres over drier and wetter years that included hurricanes, two El Nino events and a La Nina event. The recently developed Unsaturated Zone Flow (UZF1) and Lake (LAK7) packages for MODFLOW-2005 were used to simulate the changing lake sizes and the extent of the groundwater catchment contributing flow to the lake. The lake area was discretized to occupy the largest surface area at the highest observed stage and then allowed to change size. Lake cells convert to land cells and receive infiltration as receding lake area exposes the underlying unsaturated zone to rainfall and recharge. The unique model conceptualization also made it possible to capture the dynamic size of the groundwater catchment contributing to lake inflows, as the surface area and volume of the lake changed during the study

  13. Water balance of a lake with floodplain buffering: Lake Tana, Blue Nile Basin, Ethiopia

    NASA Astrophysics Data System (ADS)

    Dessie, Mekete; Verhoest, Niko E. C.; Pauwels, Valentijn R. N.; Adgo, Enyew; Deckers, Jozef; Poesen, Jean; Nyssen, Jan

    2015-03-01

    Lakes are very important components of the earth's hydrological cycle, providing a variety of services for humans and ecosystem functioning. For a sustainable use of lakes, a substantial body of knowledge on their water balance is vital. We present here a detailed daily water balance analysis for Lake Tana, the largest lake in Ethiopia and the source of the Blue Nile. Rainfall on the lake is determined by Thiessen polygon procedure, open water evaporation is estimated by the Penman-combination equation and observed inflows for the gauged catchments as well as outflow data at the two lake outlets are directly used. Runoff from ungauged catchments is estimated using a simple rainfall-runoff model and runoff coefficients. Hillslope catchments and floodplains are treated separately, which makes this study unique compared to previous water balance studies. Impact of the floodplain on the lake water balance is analyzed by conducting scenario-based studies. We found an average yearly abstraction of 420 × 106 m3 or 6% of river inflows to the lake by the floodplain in 2012 and 2013. Nearly 60% of the inflow to the lake is from the Gilgel Abay River. Simulated lake levels compare well with the observed lake levels (R2 = 0.95) and the water balance can be closed with a closure error of 82 mm/year (3.5% of the total lake inflow). This study demonstrates the importance of floodplains and their influence on the water balance of the lake and the need of incorporating the effects of floodplains and water abstraction for irrigation to improve predictions.

  14. Morphological variation of siscowet lake trout in Lake Superior

    USGS Publications Warehouse

    Bronte, C.R.; Moore, S.A.

    2007-01-01

    Historically, Lake Superior has contained many morphologically distinct forms of the lake trout Salvelinus namaycush that have occupied specific depths and locations and spawned at specific times of the year. Today, as was probably the case historically, the siscowet morphotype is the most abundant. Recent interest in harvesting siscowets to extract oil containing omega-3 fatty acids will require additional knowledge of the biology and stock structure of these lightly exploited populations. The objective of this study was to determine whether shape differences exist among siscowet populations across Lake Superior and whether these shape differences can be used to infer stock structure. Morphometric analysis (truss protocol) was used to differentiate among siscowets sampled from 23 locations in Lake Superior. We analyzed 31 distance measurements among 14 anatomical landmarks taken from digital images of fish recorded in the field. Cluster analysis of size-corrected data separated fish into three geographic groups: The Isle Royale, eastern (Michigan), and western regions (Michigan). Finer scales of stock structure were also suggested. Discriminant function analysis demonstrated that head measurements contributed to most of the observed variation. Cross-validation classification rates indicated that 67–71% of individual fish were correctly classified to their region of capture. This is the first study to present shape differences associated with location within a lake trout morphotype in Lake Superior.

  15. Geophysical investigation of sentinel lakes in Lake, Seminole, Orange, and Volusia Counties, Florida

    USGS Publications Warehouse

    Reich, Christopher; Flocks, James; Davis, Jeffrey

    2012-01-01

    This study was initiated in cooperation with the St. Johns River Water Management District (SJRWMD) to investigate groundwater and surface-water interaction in designated sentinel lakes in central Florida. Sentinel lakes are a SJRWMD established set of priority water bodies (lakes) for which minimum flows and levels (MFLs) are determined. Understanding both the structure and lithology beneath these lakes can ultimately lead to a better understanding of the MFLs and why water levels fluctuate in certain lakes more so than in other lakes. These sentinel lakes have become important water bodies to use as water-fluctuation indicators in the SJRWMD Minimum Flows and Levels program and will be used to define long-term hydrologic and ecologic performance measures. Geologic control on lake hydrology remains poorly understood in this study area. Therefore, the U.S. Geological Survey investigated 16 of the 21 water bodies on the SJRWMD priority list. Geologic information was obtained by the tandem use of high-resolution seismic profiling (HRSP) and direct-current (DC) resistivity profiling to isolate both the geologic framework (structure) and composition (lithology). Previous HRSP surveys from various lakes in the study area have been successful in identifying karst features, such as subsidence sinkholes. However, by using this method only, it is difficult to image highly irregular or chaotic surfaces, such as collapse sinkholes. Resistivity profiling was used to complement HRSP by detecting porosity change within fractured or collapsed structures and increase the ability to fully characterize the subsurface. Lake Saunders (Lake County) is an example of a lake composed of a series of north-south-trending sinkholes that have joined to form one lake body. HRSP shows surface depressions and deformation in the substrate. Resistivity data likewise show areas in the southern part of the lake where resistivity shifts abruptly from approximately 400 ohm meters (ohm-m) along the

  16. Mercury accumulation in yellow perch in Wisconsin seepage lakes: Relation to lake characteristics

    USGS Publications Warehouse

    Cope, W.G.; Wiener, J.G.; Rada, R.G.

    1990-01-01

    We studied relations between lacustrine characteristics and the total mercury (Hg) content of calendar age-2 yellow perch (Perca flavescens) in 10 seepage lakes in north-central Wisconsin. Mean concentrations and burdens (masses) of Hg in whole perch varied widely among lakes, were negatively correlated with lake pH and were positively correlated with total Hg concentration in surficial profundal sediment. Approximately 80 to 90% of the variation in Hg concentration and burden in whole perch was explained with multiple regressions containing two independent variables: either lake pH or alkalinity, and Hg concentration in surficial sediment. Variation among lakes in the Hg concentration in yellow perch was unrelated to their relative rates of growth. The mean concentration of Hg in axial muscle tissue of age-5 walleyes (Stizostedion vitreum vitreum) from five of the study lakes was highly correlated with the mean concentration in whole age-2 perch in the same lakes. We hypothesized that the high Hg concentrations often seen in piscivorous fish in low-alkalinity lakes (relative to high-alkalinity lakes) is at least partly due to a greater dietary intake of Hg in such waters. Furthermore, the analysis of small yellow perch—the preferred prey of adult walleyes and an important forage species for many predatory fishes in the north-central United States—may be an effective approach to assessing Hg bioavailability in the region's lakes.

  17. Estimate of net trophic transfer efficiency of PCBs to Lake Michigan lake trout from their prey

    USGS Publications Warehouse

    Madenjian, Charles P.; Hesselberg, Robert J.; DeSorcie, Timothy J.; Schmidt, Larry J.; Stedman, Ralph M.; Quintal, Richard T.; Begnoche, Linda J.; Passino-Reader, Dora R.

    1998-01-01

    Most of the polychlorinated biphenyl (PCB) body burden accumulated by lake trout (Salvelinus namaycush) from the Laurentian Great Lakes is from their food. We used diet information, PCB determinations in both lake trout and their prey, and bioenergetics modeling to estimate the efficiency with which Lake Michigan lake trout retain PCBs from their food. Our estimates were the most reliable estimates to date because (a) the lake trout and prey fish sampled during our study were all from the same vicinity of the lake, (b) detailed measurements were made on the PCB concentrations of both lake trout and prey fish over wide ranges in fish size, and (c) lake trout diet was analyzed in detail over a wide range of lake trout size. Our estimates of net trophic transfer efficiency of PCBs to lake trout from their prey averaged from 0.73 to 0.89 for lake trout between the ages of 5 and 10 years old. There was no evidence of an upward or downward trend in our estimates of net trophic transfer efficiency for lake trout between the ages of 5 and 10 years old, and therefore this efficiency appeared to be constant over the duration of the lake trout's adult life in the lake. On the basis of our estimtes, lake trout retained 80% of the PCBs that are contained within their food.

  18. Great Lakes Literacy Principles

    NASA Astrophysics Data System (ADS)

    Fortner, Rosanne W.; Manzo, Lyndsey

    2011-03-01

    Lakes Superior, Huron, Michigan, Ontario, and Erie together form North America's Great Lakes, a region that contains 20% of the world's fresh surface water and is home to roughly one quarter of the U.S. population (Figure 1). Supporting a $4 billion sport fishing industry, plus $16 billion annually in boating, 1.5 million U.S. jobs, and $62 billion in annual wages directly, the Great Lakes form the backbone of a regional economy that is vital to the United States as a whole (see http://www.miseagrant.umich.edu/downloads/economy/11-708-Great-Lakes-Jobs.pdf). Yet the grandeur and importance of this freshwater resource are little understood, not only by people in the rest of the country but also by many in the region itself. To help address this lack of knowledge, the Centers for Ocean Sciences Education Excellence (COSEE) Great Lakes, supported by the U.S. National Science Foundation and the National Oceanic and Atmospheric Administration, developed literacy principles for the Great Lakes to serve as a guide for education of students and the public. These “Great Lakes Literacy Principles” represent an understanding of the Great Lakes' influences on society and society's influences on the Great Lakes.

  19. Climate-driven changes in grassland vegetation, snow cover, and lake water of the Qinghai Lake basin

    NASA Astrophysics Data System (ADS)

    Wang, Xuelu; Liang, Tiangang; Xie, Hongjie; Huang, Xiaodong; Lin, Huilong

    2016-07-01

    Qinghai Lake basin and the lake have undergone significant changes in recent decades. We examine MODIS-derived grassland vegetation and snow cover of the Qinghai Lake basin and their relations with climate parameters during 2001 to 2010. Results show: (1) temperature and precipitation of the Qinghai Lake basin increased while evaporation decreased; (2) most of the grassland areas improved due to increased temperature and growing season precipitation; (3) weak relations between snow cover and precipitation/vegetation; (4) a significantly negative correlation between lake area and temperature (r=-0.9, p<0.05) and (5) a positive relation between lake level (lake-level difference) and temperature (precipitation). Compared with Namco Lake (located in the inner Tibetan Plateau) where the primary water source of lake level increases was the accelerated melt of glacier/perennial snow cover in the lake basin, for the Qinghai Lake, however, it was the increased precipitation. Increased precipitation explained the improvement of vegetation cover in the Qinghai Lake basin, while accelerated melt of glacier/perennial snow cover was responsible for the degradation of vegetation cover in Namco Lake basin. These results suggest different responses to the similar warming climate: improved (degraded) ecological condition and productive capacity of the Qinghai Lake basin (Namco Lake basin).

  20. Lake whitefish and Diporeia spp. in the Great lakes: an overview

    USGS Publications Warehouse

    Nalepa, Thomas F.; Mohr, Lloyd C.; Henderson, Bryan A.; Madenjian, Charles P.; Schneeberger, Philip J.

    2005-01-01

    Because of growing concern in the Great Lakes over declines in abundance and growth of lake whitefish (Coregonus clupeaformis) and declines in abundance of the benthic amphipod Diporeia spp., a workshop was held to examine past and current trends, to explore trophic links, and to discuss the latest research results and needs. The workshop was divided into sessions on the status of populations in each of the lakes, bioenergetics and trophic dynamics, and exploitation and management. Abundance, growth, and condition of whitefish populations in Lakes Superior and Erie are stable and within the range of historical means, but these variables are declining in Lakes Michigan and Ontario and parts of Lake Huron. The loss of Diporeia spp., a major food item of whitefish, has been a factor in observed declines, particularly in Lake Ontario, but density-dependent factors also likely played a role in Lakes Michigan and Huron. The loss of Diporeia spp. is temporally linked to the introduction and proliferation of dreissenid mussels, but a direct cause for the negative response of Diporeia spp. has not been established. Given changes in whitefish populations, age-structured models need to be re-evaluated. Other whitefish research needs to include a better understanding of what environmental conditions lead to strong year-classes, improved aging techniques, and better information on individual population (stock) structure. Further collaborations between assessment biologists and researchers studying the lower food web would enhance an understanding of links between trophic levels.

  1. Lake trout (Salvelinus namaycush) populations in Lake Superior and their restoration in 1959-1993

    USGS Publications Warehouse

    Hansen, Michael J.; Peck, James W.; Schorfhaar, Richard G.; Selgeby, James H.; Schreiner, Donald R.; Schram, Stephen T.; Swanson, Bruce L.; MacCallum, Wayne R.; Burnham-Curtis, Mary K.; Curtis, Gary L.; Heinrich, John W.; Young, Robert J.

    1995-01-01

    Naturally-reproducing populations of lake trout (Salvelinus namaycush) have been reestablished in most of Lake Superior, but have not been restored to 1929-1943 average abundance. Progress toward lake trout restoration in Lake Superior is described, management actions are reviewed, and the effectiveness of those actions is evaluated; especially stocking lake trout as a tool for building spawning stocks, and subsequently, populations of wild recruits. Widespread destruction of lake trout stocks in the 1950s due to an intense fishery and sea lamprey (Petromyzon marinus) predation resulted in lower overall phenotypic diversity than was previously present. Stocking of yearling lake trout, begun in the 1950s, produced high densities of spawners that reproduced wherever inshore spawning habitat was widespread. Sea lampreys were greatly reduced, beginning in 1961, using selective chemical toxicants and barrier dams, but continue to exert substantial mortality. Fishery regulation was least effective in Wisconsin, where excessive gillnet effort caused high by-catch of lake trout until 1991, and in eastern Michigan, where lake trout restoration was deferred in favor of a tribal fishery for lake whitefish (Coregonus clupeaformis) in 1985. Restoration of stocks was quicker in offshore areas where remnant wild lake trout survived and fishing intensity was low, and was slower in inshore areas where stocked lake trout reproduced successfully and fishing intensity was high. Inshore stocks of wild lake trout are currently about 61 % of historic abundance in Michigan and 53% in Wisconsin. Direct comparison of modern and historic abundances of inshore lake trout stocks in Minnesota and Ontario is impossible due to lack of historic stock assessment data. Stocks in Minnesota are less abundant at present than in Michigan or Wisconsin, and stocks in Ontario are similar to those in Michigan. Further progress in stock recovery can only be achieved if sea lampreys are depressed and if

  2. Sources and distribution of microplastics in China's largest inland lake - Qinghai Lake.

    PubMed

    Xiong, Xiong; Zhang, Kai; Chen, Xianchuan; Shi, Huahong; Luo, Ze; Wu, Chenxi

    2018-04-01

    Microplastic pollution was studied in China's largest inland lake - Qinghai Lake in this work. Microplastics were detected with abundance varies from 0.05 × 10 5 to 7.58 × 10 5 items km -2 in the lake surface water, 0.03 × 10 5 to 0.31 × 10 5 items km -2 in the inflowing rivers, 50 to 1292 items m -2 in the lakeshore sediment, and 2 to 15 items per individual in the fish samples, respectively. Small microplastics (0.1-0.5 mm) dominated in the lake surface water while large microplastics (1-5 mm) are more abundant in the river samples. Microplastics were predominantly in sheet and fiber shapes in the lake and river water samples but were more diverse in the lakeshore sediment samples. Polymer types of microplastics were mainly polyethylene (PE) and polypropylene (PP) as identified using Raman Spectroscopy. Spatially, microplastic abundance was the highest in the central part of the lake, likely due to the transport of lake current. Based on the higher abundance of microplastics near the tourist access points, plastic wastes from tourism are considered as an important source of microplastics in Qinghai Lake. As an important area for wildlife conservation, better waste management practice should be implemented, and waste disposal and recycling infrastructures should be improved for the protection of Qinghai Lake. Copyright © 2018 Elsevier Ltd. All rights reserved.

  3. 77 FR 23123 - Special Local Regulation; Smokin The Lake; Gulfport Lake; Gulfport, MS

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-18

    ...-AA08 Special Local Regulation; Smokin The Lake; Gulfport Lake; Gulfport, MS AGENCY: Coast Guard, DHS... regulation for a portion of Gulfport Lake in Gulfport, MS. This action is necessary for the safeguard of... The Lake high speed boat races on May 5 and 6, 2012. Entry into, transiting or anchoring in this area...

  4. Sediment deposition and selected water-quality characteristics in Cedar Lake and Lake Olathe, Northeast Kansas, 2000

    USGS Publications Warehouse

    Mau, D.P.

    2002-01-01

    The Lake Olathe watershed, located in northeast Kansas, was investigated using bathymetric survey data and reservoir bottom-sediment cores to determine sediment deposition, water-quality trends, and transport of nutrients (phosphorus and nitrogen species), selected trace elements, selected pesticides, and diatoms as indicators of eutrophic (organic-enriched and depleted oxygen supply) conditions. To determine sediment deposition and loads, bathymetric data from Cedar Lake and Lake Olathe, both located in the Lake Olathe watershed, were collected in 2000 and compared to historical topographic data collected when the lakes were built. Approximately 338 acre-feet of sediment deposition has occurred in Cedar Lake since dam closure in 1938, and 317 acre-feet has occurred at Lake Olathe since 1956. Mean annual sediment deposition was 5.45 acre-feet per year (0.89 acre-feet per year per square mile) for Cedar Lake and 7.0 acre-feet per year (0.42 acre-feet per year per square mile) for Lake Olathe. Mean annual sediment loads for the two reservoirs were 9.6 million pounds per year for Cedar Lake and 12.6 million pounds per year for Lake Olathe. Mean concentrations of total phosphorus in bottom-sediment samples from Cedar Lake ranged from 1,370 to 1,810 milligrams per kilogram, and concentrations in bottom-sediment samples from Lake Olathe ranged from 588 to 1,030 milligrams per kilogram. The implication of large total phosphorus concentrations in the bottom sediment of Cedar Lake is that inflow into Cedar Lake is rich in phosphorus and that adverse water-quality conditions could affect water quality in downstream Lake Olathe through discharge of water from Cedar Lake to Lake Olathe via Cedar Creek. Mean annual phosphorus loads transported from the Lake Olathe watershed were estimated to be 14,700 pounds per year for Cedar Lake and 9,720 pounds per year for Lake Olathe. The mean annual phosphorus yields were estimated to be 3.74 pounds per acre per year for Cedar Lake and 0

  5. Earth Observations taken by the Expedition 21 Crew

    NASA Image and Video Library

    2009-11-14

    ISS021-E-026475 (14 Nov. 2009) --- Ounianga Lakes in the Sahara Desert, in the nation of Chad are featured in this image photographed by an Expedition 21 crew member on the International Space Station. This view features one of the largest of a series of ten, mostly fresh water lakes in the Ounianga basin in the heart of the Sahara Desert of northeastern Chad. According to scientists, the lakes are the remnant of a single large lake, probably tens of kilometers long that once occupied this remote area approximately 14,800 to 5,500 years ago. As the climate dried out during the subsequent millennia, the lake was reduced in size and large wind-driven sand dunes invaded the original depression dividing it into several smaller basins. The area shown in this image measures approximately 11 x 9 kilometers, with the dark water surfaces of the lake segregated almost completely by orange linear sand dunes that stream into the depression from the northeast. The almost year-round northeast winds and cloudless skies make for very high evaporation (an evaporation rate of greater than six meters per year has been measured in one of the nearby lakes). Despite this, only one of the ten lakes is saline. According to scientists, the reason for the apparent paradox of fresh water lakes in the heart of the desert lies in the fact that fresh water from a very large aquifer reaches the surface in the Ounianga depression in the form of the lakes. The aquifer is large enough to keep supplying the small lakes with water despite the high evaporation rate. Mats of floating reeds also reduce the evaporation in places. The lakes form a hydrological system that is unique in the Sahara Desert. Scientists believe the aquifer was charged with fresh water, and the large original lake evolved, during the so-called African Humid Period (approximately 14,800 to 5,500 years ago) when the West African summer monsoon was stronger than it is today. Associated southerly winds brought Atlantic moisture well

  6. Acoustic estimates of abundance and distribution of spawning lake trout on Sheboygan Reef in Lake Michigan

    USGS Publications Warehouse

    Warner, D.M.; Claramunt, R.M.; Janssen, J.; Jude, D.J.; Wattrus, N.

    2009-01-01

    Efforts to restore self-sustaining lake trout (Salvelinus namaycush) populations in the Laurentian Great Lakes have had widespread success in Lake Superior; but in other Great Lakes, populations of lake trout are maintained by stocking. Recruitment bottlenecks may be present at a number of stages of the reproduction process. To study eggs and fry, it is necessary to identify spawning locations, which is difficult in deep water. Acoustic sampling can be used to rapidly locate aggregations of fish (like spawning lake trout), describe their distribution, and estimate their abundance. To assess these capabilities for application to lake trout, we conducted an acoustic survey covering 22 km2 at Sheboygan Reef, a deep reef (<40 m summit) in southern Lake Michigan during fall 2005. Data collected with remotely operated vehicles (ROV) confirmed that fish were large lake trout, that lake trout were 1–2 m above bottom, and that spawning took place over specific habitat. Lake trout density exhibited a high degree of spatial structure (autocorrelation) up to a range of ~190 m, and highest lake trout and egg densities occurred over rough substrates (rubble and cobble) at the shallowest depths sampled (36–42 m). Mean lake trout density in the area surveyed (~2190 ha) was 5.8 fish/ha and the area surveyed contained an estimated 9500–16,000 large lake trout. Spatial aggregation in lake trout densities, similarity of depths and substrates at which high lake trout and egg densities occurred, and relatively low uncertainty in the lake trout density estimate indicate that acoustic sampling can be a useful complement to other sampling tools used in lake trout restoration research.

  7. Visualizing the geology of lake trout spawning sites, northern Lake Michigan

    USGS Publications Warehouse

    Dartnell, Peter; Barnes, Peter; Gardner, James V.; Lee, Kristen

    2004-01-01

    Geologists and biologists are working together to understand the links between lake floor geology (composition and shape) and the distribution of lake trout throughout their life cycle. Lake floor geology is one of the main factors determining where lake trout spawn, feed, and hide. In support of ongoing research to study Lake Michigan trout habitats, the U.S. Geological Survey in cooperation with the U.S. Army Corps of Engineers mapped the morphology of principle lake trout spawning sites. Using the Army Corps of Engineer's SHOALS airborne lidar (Light Detection and Ranging) system we mapped six regions in Northern Lake Michigan in order to identify ideal spawning regions composed of shallow, clean, gravel/cobble substrate, adjacent to deeper water. Lidar mapping systems, which use laser pulses to measure water depths from an airplane, are now available to map the nearshore lake morphology at meter-scale detail. Maps generated from the bathymetric data are used to define regions with smooth homogeneous substrate, regions with higher relief, and mixed regions with both smooth and rough relief. This morphologic information combined with sediment samples and direct bottom observations enable geologists to map areas with rougher relief composed of rock outcrop, boulders, and cobbles, as well as smooth regions covered with sand or mud. This information helps biologists, fishery managers, and ecologists visualize the lake floor in significant detail which promotes better fishery management, species protection, and habitat identification. These maps present the maps and discuss the geology of the six lake trout spawning sites mapped by the lidar system. Where the mapping approached land, aerial photography of the land is combined with the bathymetric data to help visualize the scale of the offshore features. Map and perspective views of Boulder Reef, Hog Island Reef, and Little Traverse Bay are shown on sheet 1, whereas map and perspective views of Trout and High Island

  8. Visualizing the geology of lake trout spawning sites; northern Lake Michigan

    USGS Publications Warehouse

    Dartnell, Peter; Barnes, Peter; Gardner, James V.; Lee, Kristen

    2006-01-01

    Geologists and biologists are working together to understand the links between lake floor geology (composition and shape) and the distribution of lake trout throughout their life cycle. Lake floor geology is one of the main factors determining where lake trout spawn, feed, and hide. In support of ongoing research to study Lake Michigan trout habitats, the U.S. Geological Survey in cooperation with the U.S. Army Corps of Engineers mapped the morphology of principle lake trout spawning sites. Using the Army Corps of Engineer's SHOALS airborne lidar (Light Detection and Ranging) system we mapped six regions in Northern Lake Michigan in order to identify ideal spawning regions composed of shallow, clean, gravel/cobble substrate, adjacent to deeper water. Lidar mapping systems, which use laser pulses to measure water depths from an airplane, are now available to map the nearshore lake morphology at meter-scale detail. Maps generated from the bathymetric data are used to define regions with smooth homogeneous substrate, regions with higher relief, and mixed regions with both smooth and rough relief. This morphologic information combined with sediment samples and direct bottom observations enable geologists to map areas with rougher relief composed of rock outcrop, boulders, and cobbles, as well as smooth regions covered with sand or mud. This information helps biologists, fishery managers, and ecologists visualize the lake floor in significant detail which promotes better fishery management, species protection, and habitat identification. These maps present the maps and discuss the geology of the six lake trout spawning sites mapped by the lidar system. Where the mapping approached land, aerial photography of the land is combined with the bathymetric data to help visualize the scale of the offshore features. Map and perspective views of Boulder Reef, Hog Island Reef, and Little Traverse Bay are shown on sheet 1, whereas map and perspective views of Trout and High Island

  9. A Killer Lake

    ERIC Educational Resources Information Center

    Horvath, Thomas

    2005-01-01

    In 1986, Lake Nyos, a volcanic lake in Cameroon, released a huge amount of carbon dioxide gas, killing over 1,700 people in the surrounding area. This case study, developed for use in a limnology or aquatic biology course, explores that event, introducing students to concepts relating to lake formation, thermal stratification, and dissolved gases.…

  10. The Tibesti Volcanoes of Chad: an ASTER-based Remote Sensing Analysis

    NASA Astrophysics Data System (ADS)

    Permenter, J. L.; Oppenheimer, C.

    2002-12-01

    Situated in the central Sahara desert, the Tibesti volcanic province of northern Chad, Africa, is a superb example of large-scale continental hot spot volcanism. The massif is comprised of seven major volcanoes and an assembly of related volcanic and tectonic structures, with a total surface area of over 350 km2. Its highest peak (Emi Koussi) rises above the surrounding desert to ~3415 m above sea level. Due, in part, to its remoteness, the Tibesti has never been described in volcanological detail. This study aims to provide the first modern synthesis of the volcanology of this significant hot spot province. It is based primarily on a detailed analysis and interpretation of a comprehensive set of multi-band imagery from NASA's Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER). ASTER has 14 spectral bands, divided between 3 optical subsystems; 3 in the very-near infrared (VNIR), 6 in the short-wave infrared, and 5 in the thermal infrared regions. In addition, the VNIR subsystem has aft-viewing optics for stereoscopic observation in the along-track direction, which permits generation of digital elevation models. The preliminary results presented here focus on the discrimination of lava composition, identification of pyroclastic deposits, and characterisation of the dimension of flows, craters, and other structural elements of the massif, using spectral and textural information gathered from the ASTER imagery. Furthermore, stratigraphic detail is obtained from the superposition of flow units and craters. The application of ASTER data to the Tibesti volcanic complex permits an initial first order description of the relative proportions and timing of different erupted materials, providing a framework for further interpretation of the volcanology and magmatic evolution of the Tibesti, based on modern geologic and tectonic concepts. It also allows intercomparisons to be made with other continental hot spot provinces.

  11. LAKE-WETLAND LINKAGE AND PERIPHYTON DYNAMICS IN A LAKE SUPERIOR COASTAL WETLAND

    EPA Science Inventory

    Tributaries feeding coastal wetlands along the Wisconsin shore of Lake Superior are generally depleted in inorganic nitrogen (TIN) relative to phosphorus (SRP), while Lake Superior is phosphorous depleted and relatively rich in TIN. Within wetlands, mixing of tributary and lake w...

  12. Comparison of filters for concentrating microbial indicators and pathogens in lake-water samples

    USGS Publications Warehouse

    Francy, Donna S.; Stelzer, Erin A.; Brady, Amie M.G.; Huitger, Carrie; Bushon, Rebecca N.; Ip, Hon S.; Ware, Michael W.; Villegas, Eric N.; Gallardo, Vincent; Lindquist, H.D. Alan

    2013-01-01

    Bacterial indicators are used to indicate increased health risk from pathogens and to make beach closure and advisory decisions; however, beaches are seldom monitored for the pathogens themselves. Studies of sources and types of pathogens at beaches are needed to improve estimates of swimming-associated health risks. It would be advantageous and cost-effective, especially for studies conducted on a regional scale, to use a method that can simultaneously filter and concentrate all classes of pathogens from the large volumes of water needed to detect pathogens. In seven recovery experiments, stock cultures of viruses and protozoa were seeded into 10-liter lake water samples, and concentrations of naturally occurring bacterial indicators were used to determine recoveries. For the five filtration methods tested, the highest median recoveries were as follows: glass wool for adenovirus (4.7%); NanoCeram for enterovirus (14.5%) and MS2 coliphage (84%); continuous-flow centrifugation (CFC) plus Virocap (CFC+ViroCap) for Escherichia coli (68.3%) and Cryptosporidium (54%); automatic ultrafiltration (UF) for norovirus GII (2.4%); and dead-end UF for Enterococcus faecalis (80.5%), avian influenza virus (0.02%), and Giardia (57%). In evaluating filter performance in terms of both recovery and variability, the automatic UF resulted in the highest recovery while maintaining low variability for all nine microorganisms. The automatic UF was used to demonstrate that filtration can be scaled up to field deployment and the collection of 200-liter lake water samples.

  13. Comparison of Filters for Concentrating Microbial Indicators and Pathogens in Lake Water Samples

    PubMed Central

    Stelzer, Erin A.; Brady, Amie M. G.; Huitger, Carrie; Bushon, Rebecca N.; Ip, Hon S.; Ware, Michael W.; Villegas, Eric N.; Gallardo, Vicente; Lindquist, H. D. Alan

    2013-01-01

    Bacterial indicators are used to indicate increased health risk from pathogens and to make beach closure and advisory decisions; however, beaches are seldom monitored for the pathogens themselves. Studies of sources and types of pathogens at beaches are needed to improve estimates of swimming-associated health risks. It would be advantageous and cost-effective, especially for studies conducted on a regional scale, to use a method that can simultaneously filter and concentrate all classes of pathogens from the large volumes of water needed to detect pathogens. In seven recovery experiments, stock cultures of viruses and protozoa were seeded into 10-liter lake water samples, and concentrations of naturally occurring bacterial indicators were used to determine recoveries. For the five filtration methods tested, the highest median recoveries were as follows: glass wool for adenovirus (4.7%); NanoCeram for enterovirus (14.5%) and MS2 coliphage (84%); continuous-flow centrifugation (CFC) plus Virocap (CFC+ViroCap) for Escherichia coli (68.3%) and Cryptosporidium (54%); automatic ultrafiltration (UF) for norovirus GII (2.4%); and dead-end UF for Enterococcus faecalis (80.5%), avian influenza virus (0.02%), and Giardia (57%). In evaluating filter performance in terms of both recovery and variability, the automatic UF resulted in the highest recovery while maintaining low variability for all nine microorganisms. The automatic UF was used to demonstrate that filtration can be scaled up to field deployment and the collection of 200-liter lake water samples. PMID:23263948

  14. Great Salt Lake, Utah

    USGS Publications Warehouse

    Stephens, Doyle W.; Gardner, Joe F.

    1999-01-01

    This document is intended as a source of general information and facts about Great Salt Lake, Utah. This U.S. Geological Survey information sheet answers frequently asked questions about Great Salt Lake. Topics include: History, salinity, brine shrimp, brine flies, migratory birds, and recreation. Great Salt Lake, the shrunken remnant of prehistoric Lake Bonneville, has no outlet. Dissolved salts accumulate in the lake by evaporation. Salinity south of the causeway has ranged from 6 percent to 27 percent over a period of 22 years (2 to 7 times saltier than the ocean). The high salinity supports a mineral industry that extracts about 2 million tons of salt from the lake each year. The aquatic ecosystem consists of more than 30 species of organisms. Harvest of its best-known species, the brine shrimp, annually supplies millions of pounds of food for the aquaculture industry worldwide. The lake is used extensively by millions of migratory and nesting birds and is a place of solitude for people. All this occurs in a lake that is located at the bottom of a 35,000-square-mile drainage basin that has a human population of more than 1.5 million.

  15. Spring-summer diet of lake trout on Six Fathom Bank and Yankee Reef in Lake Huron

    USGS Publications Warehouse

    Madenjian, C.P.; Holuszko, J.D.; Desorcie, T.J.

    2006-01-01

    We examined the stomach contents of 1,045 lake trout (Salvelinus namaycush) caught on Six Fathom Bank and Yankee Reef, two offshore reef complexes in Lake Huron, during late spring and early summer 1998-2003. Lake trout ranged in total length from 213 to 858 mm, and in age from 2 to 14 years. In total, 742 stomachs contained food. On a wet-weight basis, alewife (Alosa pseudoharengus) dominated the spring-summer diet of lake trout on both of these offshore reef complexes. Alewives accounted for 75 to 90% of lake trout diet, depending on the lake trout size category. Size of alewives found in lake trout stomachs increased with increasing lake trout size. Faster growth of juvenile lake trout on Six Fathom Bank and Yankee Reef than on Sheboygan Reef in Lake Michigan was attributed to greater availability of small alewives on the offshore reefs in Lake Huron. Our findings indicated that alewives inhabited Six Fathom Bank and Yankee Reef during spring and summer months. Thus, our study provided support for the contention that alewives may have interfered with natural reproduction by lake trout on these offshore reef complexes in Lake Huron.

  16. Water quality of Fremont Lake and New Fork Lakes, western Wyoming; a progress report

    USGS Publications Warehouse

    Peterson, D.A.; Averett, R.C.; Mora, K.L.

    1987-01-01

    Fremont Lake and New Fork Lakes in the New Fork River drainage of western Wyoming were selected for a comprehensive study of hydrologic processes affecting mountain lakes in the Rocky Mountains. Information is needed about lakes in this area to assess their response to existing and planned development. The concerns include regional issues such as acid precipitation from gas-sweetening plants, coal-fired powerplants, and smelters, as well as local issues, such as shoreline development and raising outlet control structures. Onsite measurements indicated strong thermal stratification in the lakes during the summer. Isothermal conditions occurred during December 1983 and May 1984. Mean phytoplankton concentrations were less than 5,000 cells/ml, and chlorophyll a concentrations were weakly correlated with phytoplankton concentrations. Zooplankton concentrations were small, less than 6 organisms/L. The numbers of benthic invertebrates/unit area in Fremont Lake were extremely small. The lake waters and inflow and outflow streams were chemically dilute solutions. Mean dissolved-solids concentrations were 13 mg/L in Fremont Lake and 24 mg/L in New Fork Lakes. Calcium and bicarbonate were the predominant ions. Concentrations of phosphorus and nitrogen usually were less than detection limits. Trace-metals concentrations in the lakes were similar to those in precipitation and generally were small. Dissolved organic-carbon concentrations were about 1 mg/L. Concentrations of fulvic and humic acids were relatively large in the inlet of Fremont Lake during the spring. Pine Creek has deposited 800 metric tons of sediment, on an annual average, to the delta of Fremont Lake. Most sediment is deposited during spring runoff. (USGS)

  17. Attoriolong Tradition of Lake Preservation

    NASA Astrophysics Data System (ADS)

    Mustamin, Kamaruddin; Rahmawati, Rahmawati; Abbas, Abbas; Akbar, Akbar

    2018-05-01

    Lake as a source of life for the fishermen has a very significant potential in influencing the views and behaviour of the fishing community’s culture. Events such as tidal lake erratic, flooding, high hardness water waves, even the lack of fish catches and the death of sailors in the lake, are symptoms that serve as the base outlook for the fishermen errors that they do against the unseen force which they consider to be the ruler of the lake. The fishing community of Lake Tempe has run attoriolong and established rules related to the management of the lake since the pre-Islamic period (before 1610 AD), led by a Macua Tappareng. Macua Tappareng is considered understand the ancestor details and rules in controlling the use of the lake, including the mystical of the lake. This study explores the local wisdom of coastal fishing communities of Lake Tempe in interacting with the lake led by Macua Tappareng. Macua Tappareng hereditary ensures that attoriolong tradition with all the rules and policies related to the lake is applied correctly. This research is a descriptive-analytic study that intends to describe as well as analyze the regulations and procedures on how Macua Tappareng in preserving the lake. The research shows that Macua Tappareng has the legitimacy and strategic position in protecting the lake, as evidenced by the continued preservation of some rules of fishing, such as a ban on fishing in particular place and time. Besides, the tradition of Maccera’ Tappareng is still considered liabilities fishing communities in honour of the ruler of the lake even become additional tools of the governments in disseminating and implementing local regulations.

  18. Contaminant trends in lake trout and walleye from the Laurentian Great Lakes

    USGS Publications Warehouse

    DeVault, David S.; Hesselberg, Robert J.; Rodgers, Paul W.; Feist, Timothy J.

    1996-01-01

    Trends in PCBs, DDT, and other contaminants have been monitored in Great Lakes lake trout and walleye since the 1970s using composite samples of whole fish. Dramatic declines have been observed in concentrations of PCB, ΣDDT, dieldrin, and oxychlordane, with declines initially following first order loss kinetics. Mean PCB concentrations in Lake Michigan lake trout increased from 13 μg/g in 1972 to 23 μg/g in 1974, then declined to 2.6 μg/g by 1986. Between 1986 and 1992 there was little change in concentration, with 3.5 μg/g observed in 1992. ΣDDT in Lake Michigan trout followed a similar trend, decreasing from 19.2 μg/g in 1970 to 1.1 μg/g in 1986, and 1.2 μg/g in 1992. Similar trends were observed for PCBs and ΣDDT in lake trout from Lakes Superior, Huron and Ontario. Concentrations of both PCB and ΣDDT in Lake Erie walleye declined between 1977 and 1982, after which concentrations were relatively constant through 1990. When originally implemented it was assumed that trends in the mean contaminant concentrations in open-lake fish would serve as cost effective surrogates to trends in the water column. While water column data are still extremely limited it appears that for PCBs in lakes Michigan and Superior, trends in lake trout do reasonably mimic those in the water column over the long term. Hypotheses to explain the trends in contaminant concentrations are briefly reviewed. The original first order loss kinetics used to describe the initial decline do not explain the more recent leveling off of contaminant concentrations. Recent theories have examined the possibilities of multiple contaminant pools. We suggest another hypothesis, that changes in the food web may have resulted in increased bioaccumulation. However, a preliminary exploration of this hypothesis using a change point analysis was inconclusive.

  19. Stability of lava lakes

    NASA Astrophysics Data System (ADS)

    Witham, Fred; Llewellin, Edward W.

    2006-11-01

    A physical model of a generic lava lake system is developed. We derive the requisite conditions for the existence of an 'equilibrium lava lake' in which magmastatic pressure at the base of the conduit balances the pressure in the underlying magmatic reservoir. The stability of this lava lake system is tested by investigating the response of the system to perturbation. We develop a graphical method, based on the system's pressure-depth profile, to predict the subsequent behaviour of the system. Despite the simplicity of the modelled system, we find a broad behavioural spectrum. Initially, the rise of bubbles through the magma is ignored. In this case, both stable, long-lived lava lakes, and unstable lakes that are prone to sudden draining, are predicted. The stability of the system is shown to be controlled by lake-conduit geometry, the solubility and gas expansion laws and the magma's volatile content. We show that an unstable lake must collapse to a new, stable equilibrium. Subsequent recharge of the system by, for example, conduit overturn, would promote a return to the original equilibrium, giving rise to cyclic behaviour. Such a mechanism is consistent with lava lake behaviour during the 1983-1984 Pu'u 'O'o eruption of Kilauea. When the rise of bubbles through the magma is considered, our model predicts that stable lakes must drain over time. We, therefore, deduce that persistently degassing, stable lava lakes, such as those observed at Mt. Erebus, Antarctica, and Mauna Ulu, Kilauea, Hawaii, must have an effective conduit convection mechanism or an exogenous supply of bubbles from depth.

  20. Increasing thiamine concentrations in lake trout eggs from Lakes Huron and Michigan coincide with low alewife abundance

    USGS Publications Warehouse

    Riley, Stephen C.; Rinchard, Jacques; Honeyfield, Dale C.; Evans, Allison N.; Begnoche, Linda

    2011-01-01

    Lake trout Salvelinus namaycush in the Laurentian Great Lakes suffer from thiamine deficiency as a result of adult lake trout consuming prey containing thiaminase, a thiamine-degrading enzyme. Sufficiently low egg thiamine concentrations result in direct mortality of or sublethal effects on newly hatched lake trout fry. To determine the prevalence and severity of low thiamine in lake trout eggs, we monitored thiamine concentrations in lake trout eggs from 15 sites in Lakes Huron and Michigan from 2001 to 2009. Lake trout egg thiamine concentrations at most sites in both lakes were initially low and increased over time at 11 of 15 sites, and the proportion of females with egg thiamine concentrations lower than the recommended management objective of 4 nmol/g decreased over time at eight sites. Egg thiamine concentrations at five of six sites in Lakes Huron and Michigan were significantly inversely related to site-specific estimates of mean abundance of alewives Alosa pseudoharengus, and successful natural reproduction of lake trout has been observed in Lake Huron since the alewife population crashed. These results support the hypothesis that low egg thiamine in Great Lakes lake trout is associated with increased alewife abundance and that low alewife abundance may currently be a prerequisite for successful reproduction by lake trout in the Great Lakes.

  1. Glacial lakes in the Indian Himalayas--from an area-wide glacial lake inventory to on-site and modeling based risk assessment of critical glacial lakes.

    PubMed

    Worni, Raphael; Huggel, Christian; Stoffel, Markus

    2013-12-01

    Glacial lake hazards and glacial lake distributions are investigated in many glaciated regions of the world, but comparably little attention has been given to these topics in the Indian Himalayas. In this study we present a first area-wide glacial lake inventory, including a qualitative classification at 251 glacial lakes >0.01 km(2). Lakes were detected in the five states spanning the Indian Himalayas, and lake distribution pattern and lake characteristics were found to differ significantly between regions. Three glacial lakes, from different geographic and climatic regions within the Indian Himalayas were then selected for a detailed risk assessment. Lake outburst probability, potential outburst magnitudes and associated damage were evaluated on the basis of high-resolution satellite imagery, field assessments and through the use of a dynamic model. The glacial lakes analyzed in the states of Jammu and Kashmir and Himachal Pradesh were found to present moderate risks to downstream villages, whereas the lake in Sikkim severely threatens downstream locations. At the study site in Sikkim, a dam breach could trigger drainage of ca. 16×10(6)m(3) water and generate maximum lake discharge of nearly 7000 m(3) s(-). The identification of critical glacial lakes in the Indian Himalayas and the detailed risk assessments at three specific sites allow prioritizing further investigations and help in the definition of risk reduction actions. Copyright © 2012 Elsevier B.V. All rights reserved.

  2. What can the National Lake Assessment Tell Us about Ecosystem Service Benefits in Lakes

    EPA Science Inventory

    The condition of lakes, ponds, and reservoirs is often viewed as existing along a continuum from pristine to impacted. The 2007 National Lake Survey was conducted to assess the condition of the nation’s lakes. Over 1,000 lakes were surveyed and detailed physical and chemical da...

  3. Lake Enriquillo, Dominican Republic

    NASA Image and Video Library

    2017-08-15

    Lake Enriquillo is a hypersaline lake in the Dominican Republic. In 2004, the lake covered an area of 164 square kilometers; by 2011, it had doubled in size and grown to 350 km2, inundating farmland and homes. Various reasons for the flooding include increases in rainfall; increase of sediments going into the lake, raising the lakebed; and milder temperatures, reducing surface evaporation. The lake is home to the largest population of American crocodiles in the Caribbean. The images were acquired October 26, 2003 and June 10, 2017, cover an area of 22.7 by 45.4 km, and are located at 18.5 degrees north, 71.6 degrees west. An image of Lake Enriquillo taken in 2003 is available at https://photojournal.jpl.nasa.gov/catalog/PIA21815

  4. Lake Erie...Take a Bow.

    ERIC Educational Resources Information Center

    Canning, Maureen; Dunlevy, Margie

    This elementary school teaching unit was developed as a part of a series of teaching units that deal with Lake Erie. This unit was developed to enable children to: (1) identify the Great Lakes and pick out Lake Erie on a map; (2) demonstrate knowledge of Lake Erie's origin and geography; (3) list some uses of Lake Erie; and (4) give examples of…

  5. Long-Term Variability of Satellite Lake Surface Water Temperatures in the Great Lakes

    NASA Astrophysics Data System (ADS)

    Gierach, M. M.; Matsumoto, K.; Holt, B.; McKinney, P. J.; Tokos, K.

    2014-12-01

    The Great Lakes are the largest group of freshwater lakes on Earth that approximately 37 million people depend upon for fresh drinking water, food, flood and drought mitigation, and natural resources that support industry, jobs, shipping and tourism. Recent reports have stated (e.g., the National Climate Assessment) that climate change can impact and exacerbate a range of risks to the Great Lakes, including changes in the range and distribution of certain fish species, increased invasive species and harmful algal blooms, declining beach health, and lengthened commercial navigation season. In this study, we will examine the impact of climate change on the Laurentian Great Lakes through investigation of long-term lake surface water temperatures (LSWT). We will use the ATSR Reprocessing for Climate: Lake Surface Water Temperature & Ice Cover (ARC-Lake) product over the period 1995-2012 to investigate individual and interlake variability. Specifically, we will quantify the seasonal amplitude of LSWTs, the first and last appearances of the 4°C isotherm (i.e., an important identifier of the seasonal evolution of the lakes denoting winter and summer stratification), and interpret these quantities in the context of global interannual climate variability such as ENSO.

  6. Side-scan sonar mapping of lake trout spawning habitat in northern Lake Michigan

    USGS Publications Warehouse

    Edsall, Thomas A.; Poe, Thomas P.; Nester, Robert T.; Brown, Charles L.

    1989-01-01

    Native stocks of lake trout Salvelinus namaycush were virtually or completely extirpated from the lower four Great Lakes by the early 1960s. The failure of early attempts to reestablish self-sustaining populations of lake trout was attributed partly to the practice of stocking hatcheryreared juveniles at locations and over substrates that had not been used in the past for spawning by native fish. Subsequent attempts to improve the selection of stocking locations were impeded by the lack of reliable information on the distribution of substrates on historical spawning grounds. Here we demonstrate the potential of side-scan sonar to substantially expand the data base needed to pinpoint the location of substrates where lake trout eggs, fry, or juveniles could be stocked to maximize survival and help ensure that survivors returning to spawn would encounter suitable substrates. We also describe the substrates and bathymetry of large areas on historical lake trout spawning grounds in the Fox Island Lake Trout Sanctuary in northern Lake Michigan. These areas could be used to support a contemporary self-sustaining lake trout population in the sanctuary and perhaps also in adjacent waters.

  7. Sexual difference in mercury concentrations of lake trout (Salvelinus namaycush) from Lake Ontario

    USGS Publications Warehouse

    Madenjian, C.P.; Keir, M.J.; Whittle, D.M.

    2011-01-01

    We determined total mercury (Hg) concentrations in 50 female lake trout (Salvelinus namaycush) and 69 male lake trout from Lake Ontario (Ontario, Canada and New York, United States). Results showed that, on average, males were 8% higher in Hg concentration than females in Lake Ontario. We also used bioenergetics modeling to determine whether a sexual difference in gross growth efficiency (GGE) could explain the observed sexual difference in Hg concentrations. According to the bioenergetics modeling results, male GGE was about 3% higher than female GGE, on average. Although the bioenergetics modeling could not explain the higher Hg concentrations exhibited by the males, a sexual difference in GGE remained a plausible explanation for the sexual difference in Hg concentrations of the lake trout. In an earlier study, male lake trout from Lake Ontario were found to be 22% higher in polychlorinated biphenyl (PCB) concentration than females from Lake Ontario. Thus, although males were higher in both Hg and PCB concentrations, the degree of the sexual difference in concentration varied between the two contaminants. Further research on sexual differences in Hg excretion rates and Hg direct uptake rates may be needed to resolve the disparity in results between the two contaminants.

  8. Recent lake ice-out phenology within and among lake districts of Alaska, U.S.A.

    USGS Publications Warehouse

    Arp, Christopher D.; Jones, Benjamin M.; Grosse, Guido

    2013-01-01

    The timing of ice-out in high latitudes is a fundamental threshold for lake ecosystems and an indicator of climate change. In lake-rich regions, the loss of ice cover also plays a key role in landscape and climatic processes. Thus, there is a need to understand lake ice phenology at multiple scales. In this study, we observed ice-out timing on 55 large lakes in 11 lake districts across Alaska from 2007 to 2012 using satellite imagery. Sensor networks in two lake districts validated satellite observations and provided comparison with smaller lakes. Over this 6 yr period, the mean lake ice-out for all lakes was 27 May and ranged from 07 May in Kenai to 06 July in Arctic Coastal Plain lake districts with relatively low inter-annual variability. Approximately 80% of the variation in ice-out timing was explained by the date of 0°C air temperature isotherm and lake area. Shoreline irregularity, watershed area, and river connectivity explained additional variation in some districts. Coherence in ice-out timing within the lakes of each district was consistently strong over this 6 yr period, ranging from r-values of 0.5 to 0.9. Inter-district analysis of coherence also showed synchronous ice-out patterns with the exception of the two arctic coastal districts where ice-out occurs later (June–July) and climatology is sea-ice influenced. These patterns of lake ice phenology provide a spatially extensive baseline describing short-term temporal variability, which will help decipher longer term trends in ice phenology and aid in representing the role of lake ice in land and climate models in northern landscapes.

  9. Mladotice Lake, Czechia: The unique genesis and evolution of the lake basin

    NASA Astrophysics Data System (ADS)

    Janský, Bohumír; Šobr, Miroslav; Kliment, Zdeněk; Chalupová, Dagmar

    2016-04-01

    The Mladotice Lake is a lake of unique genetic type in Czechia. In May 1872 a landslide as a result an extreme rainfall event occurred in western Czechia, blocking the Mladoticky stream valley and creating the Mladotice Lake. The 1952 and 1975 air images document that collective farming had a great impact on the lake basin evolution when balks and field terraces were removed and fields were made much larger. Because of this change in land use we expected higher soil erosion and a related increase in the sedimentation rate. First bathymetric measurements of the newly created lake were carried out in 1972 and were repeated in 1999, in 2003 and in 2014. Our analysis of the sedimentary record aims to identify the sediment stratigraphy, its basic physical and chemical properties, isotope content and thin sections yield a detailed temporal resolution of the sedimentation chronology. In some areas a sediment thickness of 4 m was detected. Hence, the average sedimentation rate is from 2.2 to 2.7 cm per year. KEY WORDS: Mladotice Lake - extreme rainfall event - landslide - land use changes - flood events - bathymetric measurements - sedimentation dynamics - stratigraphy and geochemistry of lake sediments - analyses of isotopes - sedimentation rates.

  10. Lake Garda, Italy

    NASA Image and Video Library

    2001-07-21

    This ASTER image was acquired on July 29, 2000 and covers an area of 30 by 57 km in northern Italy. Lake Garda was formed by glaciers during the last Ice Age, and is Italy's largest lake. Lago di Garda lies in the provinces of Verona, Brescia, and Trento, and is 51 kilometers (32 miles) long and from 3 to 18 kilometers (2 to 11 miles) wide. The Sarca is its chief affluent, and the lake is drained southward by the Mincio, which discharges into the Po River. Many villas are situated on its shores. On the peninsula of Sirmione, at the southern end of the lake, are the ruins of a Roman villa and a castle of the Scaligers, an Italian family of the 16th century. The RIGHT image has the land area masked out, and a harsh stretch was applied to the lake values to display variations in sediment load. Also visible are hundreds of boats and their wakes, criss-crossing the lake. The image is centered at 45.6 degrees north latitude, 10.6 degrees east longitude. http://photojournal.jpl.nasa.gov/catalog/PIA02671

  11. Airborne lidar detection and mapping of invasive lake trout in Yellowstone Lake.

    PubMed

    Roddewig, Michael R; Churnside, James H; Hauer, F Richard; Williams, Jacob; Bigelow, Patricia E; Koel, Todd M; Shaw, Joseph A

    2018-05-20

    The use of airborne lidar to survey fisheries has not yet been extensively applied in freshwater environments. In this study, we investigated the applicability of this technology to identify invasive lake trout (Salvelinus namaycush) in Yellowstone Lake, Yellowstone National Park, USA. Results of experimental trials conducted in 2004 and in 2015-16 provided lidar data that identified groups of fish coherent with current knowledge and models of lake trout spawning sites, and one identified site was later confirmed to have lake trout.

  12. Patterns in Benthic Biodiversity Link Lake Trophic Status to Structure and Potential Function of Three Large, Deep Lakes

    PubMed Central

    Hayford, Barbara L.; Caires, Andrea M.; Chandra, Sudeep; Girdner, Scott F.

    2015-01-01

    Relative to their scarcity, large, deep lakes support a large proportion of the world’s freshwater species. This biodiversity is threatened by human development and is in need of conservation. Direct comparison of biodiversity is the basis of biological monitoring for conservation but is difficult to conduct between large, insular ecosystems. The objective of our study was to conduct such a comparison of benthic biodiversity between three of the world’s largest lakes: Lake Tahoe, USA; Lake Hövsgöl, Mongolia; and Crater Lake, USA. We examined biodiversity of common benthic organism, the non-biting midges (Chironomidae) and determined lake trophic status using chironomid-based lake typology, tested whether community structure was similar between the three lakes despite geographic distance; and tested whether chironomid diversity would show significant variation within and between lakes. Typology analysis indicated that Lake Hövsgöl was ultra-oligotrophic, Crater Lake was oligotrophic, and Lake Tahoe was borderline oligotrophic/mesotrophic. These results were similar to traditional pelagic measures of lake trophic status for Lake Hövsgöl and Crater Lake but differed for Lake Tahoe, which has been designated as ultra-oligotrophic by traditional pelagic measures such as transparency found in the literature. Analysis of similarity showed that Lake Tahoe and Lake Hövsgöl chironomid communities were more similar to each other than either was to Crater Lake communities. Diversity varied between the three lakes and spatially within each lake. This research shows that chironomid communities from these large lakes were sensitive to trophic conditions. Chironomid communities were similar between the deep environments of Lake Hövsgöl and Lake Tahoe, indicating that chironomid communities from these lakes may be useful in comparing trophic state changes in large lakes. Spatial variation in Lake Tahoe’s diversity is indicative of differential response of chironomid

  13. Status of lake trout rehabilitation on Six Fathom Bank and Yankee Reef in Lake Huron

    USGS Publications Warehouse

    Madenjian, Charles P.; DeSorcie, Timothy J.; McClain, Jerry R.; Woldt, Aaron P.; Holuszko, Jeffrey D.; Bowen, Charles A.

    2004-01-01

    Six Fathom Bank, an offshore reef in the central region of Lake Huron's main basin, was stocked annually with hatchery-reared lake trout Salvelinus namaycush during 1985–1998, and nearby Yankee Reef was stocked with hatchery-reared lake trout in 1992, 1997, and annually during 1999–2001. We conducted gill-net surveys during spring and fall to evaluate performances of each of the various strains of lake trout, as well as the performance of the entire lake trout population (all strains pooled), on these two offshore reefs during 1992–2000. Criteria to evaluate performance included the proportion of “wild” fish within the population, spawner density, adult survival, growth, maturity, and wounding rate by sea lamprey Petromyzon marinus. Although naturally reproduced age-0 lake trout fry were caught on Six Fathom Bank and Yankee Reef, wild lake trout did not recruit to the adult population to any detectable degree. The density of spawning lake trout on Six Fathom Bank (>100 fish/305 m of gill net) during 1995–1998 appeared to be sufficiently high to initiate a self-sustaining population. However, annual mortality estimates for all lake trout strains pooled from catch curve analyses ranged from 0.48 to 0.62, well exceeding the target level of 0.40 suggested for lake trout rehabilitation. Annual mortality rate for the Seneca Lake strain (0.34) was significantly lower than that for the Superior–Marquette (0.69) and Lewis Lake (0.69) strains. This disparity in survival among strains was probably attributable to the lower sea-lamprey-induced mortality experienced by the Seneca Lake strain. The relatively high mortality experienced by adult lake trout partly contributed to the lack of successful natural recruitment to the adult population on these offshore reefs, but other factors were probably also involved. We recommend that both stocking of the Seneca Lake strain and enhanced efforts to reduce sea lamprey abundance in Lake Huron be continued.

  14. Temperate Lakes Discovered on Titan

    NASA Astrophysics Data System (ADS)

    Vixie, Graham; Barnes, Jason W.; Jackson, Brian; Wilson, Paul

    2012-04-01

    We have discovered two temperate lakes on Titan using Cassini's Visual and Infrared Mapping Spectrometer (VIMS). Three key features help to identify these surface features as lakes: morphology, albedo, and specular reflection. The presence of lakes at the mid-latitudes mean liquid can accumulate and remain stable outside of the poles. We first identify a lake surface by looking for possible shorelines with a lacustrine morphology. Then, we apply a simple atmospheric correction that produces an approximate surface albedo. Next, we prepare cylindrical projection maps of the brightness of the sky as seen from any points on the surface to identify specular reflections. Our techniques can then be applied to other areas, such as Arrakis Planitia, to test for liquid. Currently, all the known lakes on Titan are concentrated at the poles. Lakes have been suggested in the tropic zone by Griffith et al. Our discovery of non-transient, temperate lakes has important implications for Titan's hydrologic cycle. Clouds have been recorded accumulating in the mid-latitudes and areas have been darkened by rainfall but later brightened after evaporation (Turtle et al. 2011). Stable temperate lakes would affect total rainfall, liquid accumulation, evaporation rates, and infiltration. Polaznik Macula (Figure 1) is a great candidate for lake filling, evaporation rates, and stability. References: Griffith, C., et al.: "Evidence for Lakes on Titan's Tropical Surface". AAS/Division for Planetary Sciences Meeting Abstracts #42, Vol. 42, pp. 1077, 2010. Turtle, E. P., et al.: "Rapid and Extensive Surface Changes Near Titan's Equator: Evidence of April Showers". Science, Vol. 331, pp. 1414-, 2011. Figure 1: Polaznik Macula is the large, dark area central to the figure. The encircled dark blue areas represent positively identified lake regions in the T66 flyby. The light blue areas represent lake candidates still under analysis. The green circle marks a non-lake surface feature enclosed by a

  15. 14 CFR 93.69 - Special requirements, Lake Campbell and Sixmile Lake Airports.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC AND GENERAL OPERATING RULES SPECIAL AIR TRAFFIC RULES... person operating an aircraft to or from Lake Campbell or Sixmile Lake Airport shall conform to the flow of traffic for the Lake operations that are depicted on the appropriate aeronautical charts. ...

  16. 14 CFR 93.69 - Special requirements, Lake Campbell and Sixmile Lake Airports.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC AND GENERAL OPERATING RULES SPECIAL AIR TRAFFIC RULES... person operating an aircraft to or from Lake Campbell or Sixmile Lake Airport shall conform to the flow of traffic for the Lake operations that are depicted on the appropriate aeronautical charts. ...

  17. 14 CFR 93.69 - Special requirements, Lake Campbell and Sixmile Lake Airports.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ..., DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC AND GENERAL OPERATING RULES SPECIAL AIR TRAFFIC RULES... person operating an aircraft to or from Lake Campbell or Sixmile Lake Airport shall conform to the flow of traffic for the Lake operations that are depicted on the appropriate aeronautical charts. ...

  18. 14 CFR 93.69 - Special requirements, Lake Campbell and Sixmile Lake Airports.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ..., DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC AND GENERAL OPERATING RULES SPECIAL AIR TRAFFIC RULES... person operating an aircraft to or from Lake Campbell or Sixmile Lake Airport shall conform to the flow of traffic for the Lake operations that are depicted on the appropriate aeronautical charts. ...

  19. 14 CFR 93.69 - Special requirements, Lake Campbell and Sixmile Lake Airports.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC AND GENERAL OPERATING RULES SPECIAL AIR TRAFFIC RULES... person operating an aircraft to or from Lake Campbell or Sixmile Lake Airport shall conform to the flow of traffic for the Lake operations that are depicted on the appropriate aeronautical charts. ...

  20. The Volume of Earth's Lakes

    NASA Astrophysics Data System (ADS)

    Cael, B. B.

    How much water do lakes on Earth hold? Global lake volume estimates are scarce, highly variable, and poorly documented. We develop a mechanistic null model for estimating global lake mean depth and volume based on a statistical topographic approach to Earth's surface. The volume-area scaling prediction is accurate and consistent within and across lake datasets spanning diverse regions. We applied these relationships to a global lake area census to estimate global lake volume and depth. The volume of Earth's lakes is 199,000 km3 (95% confidence interval 196,000-202,000 km3) . This volume is in the range of historical estimates (166,000-280,000 km3) , but the overall mean depth of 41.8 m (95% CI 41.2-42.4 m) is significantly lower than previous estimates (62 - 151 m). These results highlight and constrain the relative scarcity of lake waters in the hydrosphere and have implications for the role of lakes in global biogeochemical cycles. We also evaluate the size (area) distribution of lakes on Earth compared to expectations from percolation theory. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship Program under Grant No. 2388357.

  1. Response of slimy sculpins to predation by juvenile lake trout in southern Lake Ontario

    USGS Publications Warehouse

    Owens, Randall W.; Bergstedt, Roger A.

    1994-01-01

    Abundance and biomass of slimy sculpin Cottus cognatus declined in Lake Ontario at depths most frequently occupied by juvenile lake trout Salvelinus namaycush (<70 m), but not at greater depths, during 1980–1987. The abundance of juvenile lake trout increased at depths less than 70 m between 1980 and 1987, and slimy sculpin abundance was negatively correlated with lake trout abundance. The size of slimy sculpins caught at depths less than 70 m decreased between 1980 and 1987, fish 50–99 mm becoming less common and fish 100 mm or longer becoming rare. The size of slimy sculpins at depths greater than 70 m did not change, Because slimy sculpins are the principal fish eaten by juvenile lake trout, and because juvenile lake trout were most abundant at depths where the greatest changes in the slimy sculpin population took place, we conclude that juvenile lake trout in Lake Ontario altered the slimy sculpin population. No significant negative correlations were found between abundance of slimy sculpins and those of the two most abundant fishes in Lake Ontario: Alewife Alosa pseudoharengus and rainbow smeltOsmerus mordax.

  2. Real-estate lakes

    USGS Publications Warehouse

    Rickert, David A.; Spieker, Andrew Maute

    1971-01-01

    Since the dawn of civilization waterfront land has been an irresistible attraction to man. Throughout history he has sought out locations fronting on oceans, rivers, and lakes. Originally sought for proximity .to water supply and transportation, such locations are now sought more for their esthetic qualities and for recreation. Usable natural waterfront property is limited, however, and the more desirable sites in many of our urban areas have already been taken. The lack of available waterfront sites has led to the creation of many artificial bodies of water. The rapid suburbanization that has characterized urban growth in America since the end of World War II, together with increasing affluence and le-isure time, has created a ready market for waterfront property. Accordingly, lake-centered subdivisions and developments dot the suburban landscape in many of our major urban areas. Literally thousands of lakes surrounded by homes have materialized during this period of rapid growth. Recently, several "new town" communities have been planned around this lake-centered concept. A lake can be either an asset or a liaoility to a community. A clean, clear, attractively landscaped lake is a definite asset, whereas a weed-choked, foul-smelling mudhole is a distinct liability. The urban environment poses both problems and imaginative opportunities in the development of lakes. Creation of a lake causes changes in all aspects of the environment. Hydrologic systems and ecological patterns are usually most severely altered. The developer should be aware of the potential changes; it is not sufficient merely to build a dam across a stream or to dig a hole in the ground. Development of Gl a successful lake requires careful planning for site selection and design, followed by thorough and cc ntinual management. The purpose of this report is to describe the characteristics of real-estate lakes, to pinpoint potential pmblems, and to suggest possible planning and management guidelines

  3. Circulation and sedimentation in a tidal-influenced fjord lake: Lake McKerrow, New Zealand

    NASA Astrophysics Data System (ADS)

    Pickrill, R. A.; Irwin, J.; Shakespeare, B. S.

    1981-01-01

    Lake McKerrow is a tide-influenced fjord lake, separated from the open sea by a Holocene barrier spit. Fresh, oxygenated waters of the epilimnion overlie saline, deoxygenated waters of the hypolimnion. During winter, water from the Upper Hollyford River interflows along the pycnocline, depositing coarse silt on the steep delta and transporting finer sediment down-lake. An extensive sub-lacustrine channel system on the foreset delta slope is possibly maintained by turbidity currents. Saline waters of the hypolimnion are periodically replenished. During high tides and low lake levels saline water flows into the lake and downslope into the lake basin as a density current in a well defined channel.

  4. Lake Eyre

    Atmospheric Science Data Center

    2013-04-16

    ... lake, and becomes dense with birds, frogs and colorful plant life. The Lake responds to extended dry periods (often associated with El Niño ... NASA's Jet Propulsion Laboratory, Pasadena, CA, for NASA's Science Mission Directorate, Washington, D.C. The Terra spacecraft is managed ...

  5. Hydrogeologic controls on the groundwater interactions with an acidic lake in karst terrain, Lake Barco, Florida

    USGS Publications Warehouse

    Lee, T.M.

    1996-01-01

    Transient groundwater interactions and lake stage were simulated for Lake Barco, an acidic seepage lake in the mantled karst of north central Florida. Karst subsidence features affected groundwater flow patterns in the basin and groundwater fluxes to and from the lake. Subsidence features peripheral to the lake intercepted potential groundwater inflow and increased leakage from the shallow perimeter of the lake bed. Simulated groundwater fluxes were checked against net groundwater flow derived from a detailed lake hydrologic budget with short-term lake evaporation computed by the energy budget method. Discrepancies between modeled and budget-derived net groundwater flows indicated that the model underestimated groundwater inflow, possibly contributed to by transient water table mounding near the lake. Recharge from rainfall reduced lake leakage by 10 to 15 times more than it increased groundwater inflow. As a result of the karst setting, the contributing groundwater basin to the lake was 2.4 ha for simulated average rainfall conditions, compared to the topographically derived drainage basin area of 81 ha. Short groundwater inflow path lines and rapid travel times limit the contribution of acid-neutralizing solutes from the basin, making Lake Barco susceptible to increased acidification by acid rain.

  6. Genetic variability among lake whitefish from Isle Royale and the Upper Great Lakes

    USGS Publications Warehouse

    Stott, Wendylee; Todd, Thomas N.; Kallemeyn, Larry

    2004-01-01

    The coregonine fishes from Isle Royale National Park represent a unique group that has escaped the successional changes observed elsewhere in North America. Analysis of microsatellite DNA loci revealed significant genetic differences among samples of lake whitefish (Coregonus clupeaformis) from Isle Royale, Lake Superior, and Lake Huron. The amount of genetic variation observed is consistent with that seen in other studies of whitefishes from North America. The lake whitefish from Isle Royale had previously been assigned sub-species status, but no evidence was found to support this. The effects of common ancestry and demographics both play a role in determining the relatedness of the populations. As with other fish species from Isle Royale and the upper Great Lakes, the lake whitefish have their origins in the Mississippi refugium.

  7. Incidence of somatic and F+ coliphage in Great Lake Basin recreational waters.

    PubMed

    Wanjugi, Pauline; Sivaganesan, Mano; Korajkic, Asja; McMinn, Brian; Kelty, Catherine A; Rhodes, Eric; Cyterski, Mike; Zepp, Richard; Oshima, Kevin; Stachler, Elyse; Kinzelman, Julie; Kurdas, Stephan R; Citriglia, Mark; Hsu, Fu-Chih; Shanks, Orin C

    2018-04-25

    There is a growing interest for the use of coliphage as an alternative indicator to assess fecal pollution in recreational waters. Coliphage are a group of viruses that infect Escherichia coli and are considered as potential surrogates to infer the likely presence of enteric viral pathogens. We report the use of a dead-end hollow fiber ultrafiltration single agar layer method to enumerate F+ and somatic coliphage from surface waters collected from three Great Lake areas. At each location, three sites (two beaches; one river) were sampled five days a week over the 2015 beach season (n = 609 total samples). In addition, culturable E. coli and enterococci concentrations, as well as 16 water quality and recreational area parameters were assessed such as rainfall, turbidity, dissolved oxygen, pH, and ultra violet absorbance. Overall, somatic coliphage levels ranged from non-detectable to 4.39 log 10 plaque forming units per liter and were consistently higher compared to F+ (non-detectable to 3.15 log 10  PFU/L), regardless of sampling site. Coliphage concentrations weakly correlated with cultivated fecal indicator bacteria levels (E. coli and enterococci) at 75% of beach sites tested in study (r = 0.28 to 0.40). In addition, ultraviolet light absorption and water temperature were closely associated with coliphage concentrations, but not fecal indicator bacteria levels suggesting different persistence trends in Great Lake waters between indicator types (bacteria versus virus). Finally, implications for coliphage water quality management and future research directions are discussed. Copyright © 2018. Published by Elsevier Ltd.

  8. Water quality of selected lakes in Mount Rainier National Park, Washington with respect to lake acidification

    USGS Publications Warehouse

    Turney, G.L.; Dion, N.P.; Sumioka, S.S.

    1986-01-01

    Thirteen lakes in Mount Rainier National Park were evaluated for general chemical characteristics, sensitivity to acidification by acidic precipitation, and degree of existing acidification. The lakes studies were Allen, one of the Chenuis group, Crescent , Crystal, Eleanor, Fan, one of the Golden group, Marsh, Mowich, Mystic, Shriner, and two unnamed lakes. The lakes were sampled in August 1983. Specific conductance values were generally 21 microsiemens/cm at 25 C or less, and dissolved solids concentrations were generally 20 mg/L or less. The major cations were calcium and sodium, and the major anion was bicarbonate. Alkalinity concentrations ranged from 2.1 to 9.0 mg/L in 12 of the lakes. Allen Lake was the exception, having an alkalinity concentration of 27 mg/L. The pH values for all of the lakes ranged from 5.8 to 6.5. In most of the lakes, vertical profiles of temperature, dissolved oxygen, pH, and specific conductance were relatively uniform. In the deeper lakes, temperature decreased with depth and dissolved-oxygen concentrations increased to about 20 feet, remained constant to 80 ft, then decreased with increasing depth. Exceptions to general water quality patterns were observed in three lakes. Allen Lake had a specific conductance value of 58 Microsiemens/cm. The lake of the Golden group was anaerobic at the bottom and had relatively high concentrations of dissolved organic carbon and dissolved metals, and a lower light transmission than the other lakes studied. One of the unnamed lakes had relatively high concentrations of phytoplankton and dissolved organic carbon and relatively low levels of light transmission. Comparisons of lake data to acid-sensitivity thresholds for specific conductance and alkalinity indicated that all of the lakes except Allen would be sensitive to acidic precipitation. The small sizes of the lakes, and their locations in basins of high precipitation and weathering-resistant rock types, enhance their sensitivity. None of the

  9. Discrimination among spawning concentrations of Lake Superior lake herring based on trace element profiles in sagittae

    USGS Publications Warehouse

    Bronte, Charles R.; Hesselberg, Robert J.; Shoesmith, John A.; Hoff, Michael H.

    1996-01-01

    Little is known about the stock structure of lake herring Coregonus artedi in Lake Superior, and recent increases in harvestable stock sizes has led to expanded exploitation in some areas. Research on marine teleosts has demonstrated that chemical differences in sagittal otoliths can be used for identification of fish stocks. We used plasma emission spectrophotometry to measure the concentrations of 10 trace elements in the sagittal otoliths from lake herring captured at eight spawning sites in Lake Superior and from Little Star Lake, an inland lake outside the Lake Superior basin. Discriminant function analysis indicated that elemental concentrations provided site-specific information but that considerable overlap existed among some locations, especially those in western Lake Superior. Correct classification rates varied from 12.0% to 86.1% and were generally higher for spawning locations from embayments in eastern Lake Superior and for the outgroup population from Little Star Lake. The results presented here demonstrate the potential usefulness of this technique for strictly freshwater species, especially those that live in highly oligotrophic waters such as Lake Superior.

  10. Limnology of Botos Lake, a tropical crater lake in Costa Rica.

    PubMed

    Umaña, G

    2001-12-01

    Botos Lake, located at the Poas Volcano complex (Costa Rica) was sampled eight times from 1994 to 1996 for physicochemical conditions of the water column and phytoplanktonic community composition. Depth was measured at fixed intervals in several transects across the lake to determine its main morphometric characteristics. The lake has an outlet to the north. It is located 2580 m above sea level and is shallow, with a mean depth of 1.8 m and a relative depth of 2.42 (surface area 10.33 ha, estimated volume 47.3 hm3). The lake showed an isothermal water column in all occasions, but it heats and cools completely according to weather fluctuations. Water transparency reached the bottom on most occasions (> 9 m). The results support the idea that the lake is polymictic and oligotrophic. The lake has at least 23 species of planktonic algae, but it was always dominated by dinoflagellates, especially Peridinium inconspicuum. The shore line is populated by a sparse population of Isoetes sp. and Eleocharis sp. mainly in the northern shore where the bottom has a gentle slope and the forest does not reach the shore.

  11. Antibiotics and Antibiotic Resistance Genes in Sediment of Honghu Lake and East Dongting Lake, China.

    PubMed

    Yang, Yuyi; Cao, Xinhua; Lin, Hui; Wang, Jun

    2016-11-01

    Sediment is an ideal medium for the aggregation and dissemination of antibiotics and antibiotic resistance genes (ARGs). The levels of antibiotics and ARGs in Honghu Lake and East Dongting Lake of central China were investigated in this study. The concentrations of eight antibiotics (four sulfonamides and four tetracyclines) in Honghu Lake were in the range 90.00-437.43 μg kg -1 (dry weight (dw)) with mean value of 278.21 μg kg -1 dw, which was significantly higher than those in East Dongting Lake (60.02-321.04 μg kg -1 dw, mean value of 195.70 μg kg -1 dw). Among the tested three sulfonamide resistance genes (sul) and eight tetracycline resistance genes (tet), sul1, sul2, tetA, tetC, and tetM had 100 % detection frequency in sediment samples of East Dongting Lake, while only sul1, sul2, and tetC were observed in all samples of Honghu Lake. The relative abundance of sul2 was higher than that of sul1 at p < 0.05 level in both lakes. The relative abundance of tet genes in East Dongting Lake was in the following order: tetM > tetB > tetC > tetA. The relative abundance of sul1, sul2, and tetC in East Dongting Lake was significantly higher than those in Honghu Lake. The abundance of background bacteria may play an important role in the horizontal spread of sul2 and tetC genes in Honghu Lake and sul1 in East Dongting Lake, respectively. Redundancy analysis indicated that tetracyclines may play a more important role than sulfonamides in the abundance of sul1, sul2, and tetC gens in Honghu Lake and East Dongting Lake.

  12. Functional convergence among pelagic sculpins of Lake Baikal and deepwater ciscoes of the Great Lakes

    USGS Publications Warehouse

    Eshenroder, Randy L.; Sideleva, Valentina G.; Todd, Thomas N.

    1999-01-01

    The vast, well-oxygenated hypolimnia of Lake Baikal and the Great Lakes were both dominated by endemic planktivorous fishes. These dominants, two species of sculpins (Comephorus, Comephoridae) in Lake Baikal and six species of deepwater ciscoes (Coregonus, Salmonidae) in the Great Lakes, although distant taxonomically, have morphologies suggesting a surprising degree of functional convergence. Here it is proposed that the same two buoyancy-regulation strategies observed in Baikal sculpins also arose in the deepwater ciscoes of the Great Lakes. One strategy favors hydrostatic lift (generated by low specific gravity) and is characterized by fatter, larger-bodied fish with smaller paired fins; the second strategy favors hydrodynamic lift (generated by swimming) and is characterized by leaner, smaller-bodied fish with larger paired fins. Both types likely evolved to feed on a single species of ecologically analogous, vertically migrating macrozooplankter: Macrohectopus branickii in Lake Baikal and Mysis relicta in the Great Lakes. It is suggested that Coregonus did not diversify and proliferate in Lake Baikal as they did in the Great Lakes because by the time Coregonus colonized Lake Baikal, pelagic sculpins were already dominant.

  13. Lake Constance

    Atmospheric Science Data Center

    2013-04-17

    ... the left-hand side of the image is bordered by Switzerland, Germany, and Austria, and has several names. Lake Constance (also written as ... is about 70 kilometers from the lakeside town of Konstanz, Germany. An annual Celtic music festival is hosted on the Swiss shores of Lake ...

  14. LAKE FORK

    EPA Science Inventory

    The Lake Fork of the Arkansas River Watershed has been adversely affected through mining, water diversion and storage projects, grazing, logging, and other human influences over the past 120 years. It is the goals of the LFWWG to improve the health of Lake fork by addressing th...

  15. Usefulness of natural regions for lake management: Analysis of variation among lakes in northwestern Wisconsin, USA

    NASA Astrophysics Data System (ADS)

    Omernik, James M.; Rohm, Christina M.; Lillie, Richard A.; Mesner, Nancy

    1991-03-01

    A map of summer total phosphorus in lakes was compiled recently for a three-state area of the upper Midwest for purposes of identifying regional patterns of total phosphorus in lakes and attainable lake trophic state. Spatial patterns in total phosphorus from approximately 3000 lakes were studied in conjunction with maps of geographic characteristics that tend to affect phosphorus balance in lakes to identify regions of similarity in phosphorus concentrations in lakes or similarity in the mosaic of values as compared to adjacent areas. While degrees of relative homogeneity are apparent at many scales, the map was designed at a scale that would yield regions with sufficient homogeneity to be useful for lake management throughout the area. In this study, data from 210 lakes in a 1560-mi2 area in northwestern Wisconsin, sampled by the Wisconsin Department of Natural Resources in the spring of 1988 (subsequent to the compilation of the phosphorus map), were examined to: (1) substantiate the existence of the regions depicted on the map in northwest Wisconsin, (2) determine the nature and relative precision of the regional boundaries, (3) determine the relative importance of natural and anthropogenic watershed characteristics, lake types, lake area, and lake depth in explaining within-region differences in lake phosphorus, and (4) demonstrate how the regions might be used by local lake managers.

  16. Genetic assessment of strain-specific sources of lake trout recruitment in the Great Lakes

    USGS Publications Warehouse

    Page, Kevin S.; Scribner, Kim T.; Bennett, Kristine R.; Garzel, Laura M.; Burnham-Curtis, Mary K.

    2003-01-01

    Populations of wild lake trout Salvelinus namaycush have been extirpated from nearly all their historical habitats across the Great Lakes. Efforts to restore self-sustaining lake trout populations in U.S. waters have emphasized the stocking of coded-wire-tagged juveniles from six hatchery strains (Seneca Lake, Lewis Lake, Green Lake, Apostle Islands, Isle Royale, and Marquette) into vacant habitats. Strain-specific stocking success has historically been based on estimates of the survival and catch rates of coded-wire-tagged adults returning to spawning sites. However, traditional marking methods and estimates of relative strain abundance provide no means of assessing strain fitness (i.e., the realized contributions to natural recruitment) except by assuming that young-of-the-year production is proportional to adult spawner abundance. We used microsatellite genetic data collected from six hatchery strains with likelihood-based individual assignment tests (IA) and mixed-stock analysis (MSA) to identify the strain composition of young of the year recruited each year. We show that strain classifications based on IA and MSA were concordant and that the accuracy of both methods varied based on strain composition. Analyses of young-of-the-year lake trout samples from Little Traverse Bay (Lake Michigan) and Six Fathom Bank (Lake Huron) revealed that strain contributions differed significantly from estimates of the strain composition of adults returning to spawning reefs. The Seneca Lake strain contributed the majority of juveniles produced on Six Fathom Bank and more young of the year than expected within Little Traverse Bay. Microsatellite markers provided a method for accurately classifying the lake trout hatchery strains used for restoration efforts in the Great Lakes and for assessment of strain-specific reproductive success.

  17. Use of egg traps to investigate lake trout spawning in the Great Lakes

    USGS Publications Warehouse

    Schreiner, Donald R.; Bronte, Charles R.; Payne, N. Robert; Fitzsimons, John D.; Casselman, John M.

    1995-01-01

    Disk-shaped traps were used to examine egg deposition by lake trout (Salvelinus namaycush) at 29 sites in the Great Lakes. The main objectives were to; first, evaluate the disk trap as a device for sampling lake trout eggs in the Great Lakes, and second, summarize what has been learned about lake trout spawning through the use of disk traps. Of the 5,085 traps set, 60% were classified as functional when retrieved. Evidence of lake trout egg deposition was documented in each of the lakes studied at 14 of 29 sites. A total of 1,147 eggs were trapped. The percentage of traps functioning and catch per effort were compared among sites based on depth, timing of egg deposition, distance from shore, size of reef, and type of reef (artificial or natural). Most eggs were caught on small, shallow, protected reefs that were close to shore. Use of disk traps on large, shallow, unprotected offshore reefs or along unprotected shorelines was generally unsuccessful due to the effects of heavy wind and wave action. Making multiple lifts at short intervals, and retrieval before and re-deployment after storms are recommended for use in exposed areas. On large reefs, preliminary surveys to identify preferred lake trout spawning habitat may be required to deploy disk traps most effectively. Egg deposition by hatchery-reared fish was widespread throughout the Great Lakes, and the use of artificial structures by these fish was extensive.

  18. 75 FR 34934 - Safety Zone; Fireworks for the Virginia Lake Festival, Buggs Island Lake, Clarksville, VA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-21

    ...-AA00 Safety Zone; Fireworks for the Virginia Lake Festival, Buggs Island Lake, Clarksville, VA AGENCY... Fireworks for the Virginia Lake Festival event. This action is intended to restrict vessel traffic movement... Virginia Lake Festival, Buggs Island Lake, Clarksville, VA (a) Regulated Area. The following area is a...

  19. 78 FR 53677 - Safety Zone; Battle of Lake Erie Fireworks, Lake Erie, Put-In-Bay, OH

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-30

    ...-AA00 Safety Zone; Battle of Lake Erie Fireworks, Lake Erie, Put-In- Bay, OH AGENCY: Coast Guard, DHS... waters of Lake Erie, Put-In-Bay, Ohio. This zone is intended to restrict vessels from a portion of Lake Erie during the Battle of Lake Erie Fireworks. [[Page 53678

  20. Africa's Great Lakes in peril

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ryan, J.C.

    Three of the great lakes in East Africa are in serious danger. Over the last two decades, overfishing, pollution, and the introduction of the Nile perch, have combined to push into extinction as many as 200 fish species found in Lake Victoria. The recycling of nutrients and oxygen through living and non-living matter has been disrupted and large area of the lake are now depleted of oxygen. Overfishing has had dramatic consequences in Lake Malawi, while Lake Tanganyika's most serious problem is with sedimentation from raw sewage, soil and chemicals. International cooperation will be required to restore these lakes.

  1. 33 CFR 162.132 - Connecting waters from Lake Huron to Lake Erie; communications rules.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... monitor: (1) Channel 11 (156.55 mhz) between Lake Huron Cut Lighted Buoy 11 and Lake St. Clair Light; and (2) Channel 12 (156.60 mhz) between Lake St. Clair Light and Detroit River Light. (b) Radiotelephone... Cut Light “7” Lake Huron Cut Lighted Buoy “1” Report. Report St. Clair/Black River Junction Light...

  2. RAINBOW LAKE WILDERNESS AND FLYNN LAKE WILDERNESS STUDY AREA, WISCONSIN.

    USGS Publications Warehouse

    Cannon, W.F.; Dunn, Maynard L.

    1984-01-01

    The Rainbow Lake Wilderness and Flynn Lake Wilderness study area in Wisconsin are contiguous and were studied as a unit. The rainbow Lake Wilderness contains a demonstrated resource of about 210,000 tons of commercial-quality peat in an area of substantiated peat resource potential. The Flynn Lake Wilderness study area contains a demonstrated resource of about 300,000 tons of commercial-quality peat in an area of substantiated peat resource potential. These deposits, however, are of limited importance because larger deposits of similar material are abundant outside the areas, closer to present markets. Rocks in the subsurface contain a low-grade copper resource identified by mining company exploration drilling. Although this is an area of substantiated copper resource potential, it is a low-grade resource, thin and generally at great depth.

  3. Comparison of Two Multiplex Methods for Detection of Respiratory Viruses: FilmArray RP and xTAG RVP ▿†

    PubMed Central

    Rand, Kenneth H.; Rampersaud, Howard; Houck, Herbert J.

    2011-01-01

    We compared the FilmArray RP (Idaho Technology, Inc., Salt Lake City, UT) and the xTAG RVP (Luminex Corporation, Toronto, Canada) multiplex respiratory virus PCR methods for the detection of respiratory viruses in a set of 200 patient specimens frozen at −70°C after standard viral culture and antigen detection methods were done. Both systems detected between 40 to 50% more viruses than traditional methods, primarily rhinoviruses and human metapneumovirus. The FilmArray RP detected significantly more total viruses either alone or as part of mixed infections than the xTAG RVP, as well as an additional 21.6% more respiratory syncytial viruses. The xTAG RVP requires 5 to 6 h with 2.5 to 3 h of hands-on time, while the FilmArray RP takes about an hour with 3 to 5 min of hands-on time, making it much easier to perform. PMID:21508156

  4. Egg thiamine status of Lake Ontario salmonines 1995-2004 with emphasis on lake trout

    USGS Publications Warehouse

    Fitzsimons, J.D.; Williston, B.; Williston, G.; Brown, L.; El-Shaarawi, A.; Vandenbyllaardt, L.; Honeyfeld, D.; Tillitt, D.; Wolgamood, M.; Brown, S.B.

    2007-01-01

    Alewives (Alosa pseudoharengus), the major prey fish for Lake Ontario, contain thiaminase. They are associated with development of a thiamine deficiency in salmonines which greatly increases the potential for developing an early mortality syndrome (EMS). To assess the possible effects of thiamine deficiency on salmonine reproduction we measured egg thiamine concentrations for five species of Lake Ontario salmonines. From this we estimated the proportion of families susceptible to EMS based on whether they were below the ED20, the egg thiamine concentration associated with 20% mortality due to EMS. The ED20s were 1.52, 2.63, and 2.99 nmol/g egg for Chinook salmon (Oncorhynchus tshawytscha), lake trout (Salvelinus namaycush), and coho salmon (Oncorhynchus kisutch), respectively. Based on the proportion of fish having egg thiamine concentrations falling below the ED20, the risk of developing EMS in Lake Ontario was highest for lake trout, followed by coho (O. kisutch), and Chinook salmon, with the least risk for rainbow trout (O. mykiss). For lake trout from western Lake Ontario, mean egg thiamine concentration showed significant annual variability during 1994 to 2003, when the proportion of lake trout at risk of developing EMS based on ED20 ranged between 77 and 100%. Variation in the annual mean egg thiamine concentration for western Lake Ontario lake trout was positively related (p < 0.001, r2 = 0.94) with indices of annual adult alewife biomass. While suggesting the possible involvement of density-dependent changes in alewives, the changes are small relative to egg thiamine concentrations when alewife are not part of the diet and are of insufficient magnitude to allow for natural reproduction by lake trout.

  5. Lake Generated Microseisms at Yellowstone Lake as a Record of Ice Phenology

    NASA Astrophysics Data System (ADS)

    Mohd Mokhdhari, A. A.; Koper, K. D.; Burlacu, R.

    2017-12-01

    It has recently been shown that wave action in lakes produces microseisms, which generate noise peaks in the period range of 0.8-1.2 s as recorded by nearby seismic stations. Such noise peaks have been observed at seven seismic stations (H17A, LKWY, B208, B944, YTP, YLA, and YLT) located within 2 km of the Yellowstone Lake shoreline. Initial work using 2016 data shows that the variations in the microseism signals at Yellowstone Lake correspond with the freezing and thawing of lake ice: the seismic noise occurs more frequently in the spring, summer, and fall, and less commonly in the winter. If this can be confirmed, then lake-generated microseisms could provide a consistent measure of the freezing and melting dates of high-latitude lakes in remote areas. The seismic data would then be useful in assessing the effects of climate change on the ice phenology of those lakes. In this work, we analyze continuous seismic data recorded by the seven seismic stations around Yellowstone Lake for the years of 1995 to 2016. We generate probability distribution functions of power spectral density for each station to observe the broad elevation of energy near a period of 1 s. The time dependence of this 1-s seismic noise energy is analyzed by extracting the power spectral density at 1 s from every processed hour. The seismic observations are compared to direct measurements of the dates of ice-out and freeze-up as reported by rangers at Yellowstone National Park. We examine how accurate the seismic data are in recording the freezing and melting of Yellowstone Lake, and how the accuracy changes as a function of the number of stations used. We also examine how sensitive the results are to the particular range of periods that are analyzed.

  6. The Honey Bee Pathosphere of Mongolia: European Viruses in Central Asia.

    PubMed

    Tsevegmid, Khaliunaa; Neumann, Peter; Yañez, Orlando

    2016-01-01

    Parasites and pathogens are apparent key factors for the detrimental health of managed European honey bee subspecies, Apis mellifera. Apicultural trade is arguably the main factor for the almost global distribution of most honey bee diseases, thereby increasing chances for multiple infestations/infections of regions, apiaries, colonies and even individual bees. This imposes difficulties to evaluate the effects of pathogens in isolation, thereby creating demand to survey remote areas. Here, we conducted the first comprehensive survey for 14 honey bee pathogens in Mongolia (N = 3 regions, N = 9 locations, N = 151 colonies), where honey bee colonies depend on humans to overwinter. In Mongolia, honey bees, Apis spp., are not native and colonies of European A. mellifera subspecies have been introduced ~60 years ago. Despite the high detection power and large sample size across Mongolian regions with beekeeping, the mite Acarapis woodi, the bacteria Melissococcus plutonius and Paenibacillus larvae, the microsporidian Nosema apis, Acute bee paralysis virus, Kashmir bee virus, Israeli acute paralysis virus and Lake Sinai virus strain 2 were not detected, suggesting that they are either very rare or absent. The mite Varroa destructor, Nosema ceranae and four viruses (Sacbrood virus, Black queen cell virus, Deformed wing virus (DWV) and Chronic bee paralysis virus) were found with different prevalence. Despite the positive correlation between the prevalence of V. destructor mites and DWV, some areas had only mites, but not DWV, which is most likely due to the exceptional isolation of apiaries (up to 600 km). Phylogenetic analyses of the detected viruses reveal their clustering and European origin, thereby supporting the role of trade for pathogen spread and the isolation of Mongolia from South-Asian countries. In conclusion, this survey reveals the distinctive honey bee pathosphere of Mongolia, which offers opportunities for exciting future research.

  7. The Honey Bee Pathosphere of Mongolia: European Viruses in Central Asia

    PubMed Central

    Tsevegmid, Khaliunaa; Neumann, Peter; Yañez, Orlando

    2016-01-01

    Parasites and pathogens are apparent key factors for the detrimental health of managed European honey bee subspecies, Apis mellifera. Apicultural trade is arguably the main factor for the almost global distribution of most honey bee diseases, thereby increasing chances for multiple infestations/infections of regions, apiaries, colonies and even individual bees. This imposes difficulties to evaluate the effects of pathogens in isolation, thereby creating demand to survey remote areas. Here, we conducted the first comprehensive survey for 14 honey bee pathogens in Mongolia (N = 3 regions, N = 9 locations, N = 151 colonies), where honey bee colonies depend on humans to overwinter. In Mongolia, honey bees, Apis spp., are not native and colonies of European A. mellifera subspecies have been introduced ~60 years ago. Despite the high detection power and large sample size across Mongolian regions with beekeeping, the mite Acarapis woodi, the bacteria Melissococcus plutonius and Paenibacillus larvae, the microsporidian Nosema apis, Acute bee paralysis virus, Kashmir bee virus, Israeli acute paralysis virus and Lake Sinai virus strain 2 were not detected, suggesting that they are either very rare or absent. The mite Varroa destructor, Nosema ceranae and four viruses (Sacbrood virus, Black queen cell virus, Deformed wing virus (DWV) and Chronic bee paralysis virus) were found with different prevalence. Despite the positive correlation between the prevalence of V. destructor mites and DWV, some areas had only mites, but not DWV, which is most likely due to the exceptional isolation of apiaries (up to 600 km). Phylogenetic analyses of the detected viruses reveal their clustering and European origin, thereby supporting the role of trade for pathogen spread and the isolation of Mongolia from South-Asian countries. In conclusion, this survey reveals the distinctive honey bee pathosphere of Mongolia, which offers opportunities for exciting future research. PMID:26959221

  8. Characterization and comparison of phytoplankton in selected lakes of five Great Lakes area national parks

    USGS Publications Warehouse

    Nevers, Meredith Becker; Whitman, Richard L.

    2004-01-01

    Phytoplankton species have been widely used as indicators of lake conditions, and they may be useful for detecting changes in overall lake condition. In an attempt to inventory and monitor its natural resources, the National Park Service wants to establish a monitoring program for aquatic resources in the Great Lakes Cluster National Parks. This study sought to establish baseline information on the phytoplankton and water chemistry of selected lakes in five national parks in a preliminary effort toward establishing a long-term monitoring program. Phytoplankton and water chemistry samples were collected from ten lakes in five national parks over a two-year period. A total of 176 taxa were identified during the study. Northern lakes generally had higher Shannon-Wiener diversity and clustered together in similarity. Lakes exhibited a south to north gradient of many water chemistry variables, with northern lakes having lower hardness, sulfate, turbidity, and temperature and higher dissolved oxygen. Chloride and sulfate concentrations were the variables that best explained variation among phytoplankton in the ten lakes. A monitoring plan will have to incorporate the differences among lakes, but by coordinating the effort, comparisons within and among parks and other regions will prove useful for determining environmental change.

  9. Monitoring climate signal transfer into the varved lake sediments of Lake Czechowskie, Poland

    NASA Astrophysics Data System (ADS)

    Groß-Schmölders, Miriam; Ott, Florian; Brykała, Dariusz; Gierszewski, Piotr; Kaszubski, Michał; Kienel, Ulrike; Brauer, Achim

    2015-04-01

    In 2012 we started a monitoring program at Lake Czechowskie, Poland, because the lake comprises a long Holocene time series of calcite varves until recent times. The aim of the program is to understand how environmental and climatic conditions influence the hydrological conditions and, ultimately, the sediment deposition processes of the lake. Lake Czechowskie is located in the north of Poland in the Pomeranian Lake District and is part of the national park Tuchola Forest. The landscape and the lake is formed by the glacier retreat after the last glaciation (Weichselian). Lake Czechowskie is a typical hardwater lake and has a length of 1.4 km, an average width of 600 m and a lake surface area of ca 4 km. The maximum depth of 32 m is reached in a rather small hollow in the eastern part of the lake. Two different types of sediment traps provide sediment samples with monthly resolution from different water depths (12m, 26m). In addition, hydrological data including water temperature in different depths, water inflow, throughflow and outflow and the depth of visibility are measured. These data allow to describe strength and duration of lake mixing in spring and autumn and its influence on sedimentation. The sediment samples were analyzed with respect to their dry weight (used to calculate mean daily sediment flux), their inorganic and organic carbon contents, the stable C- and O-isotopes of organic matter and calcite as well as N-isotopes of organic matter. For selected samples dominant diatom taxa are determined. Our first results demonstrate the strong influence of the long winter with ice cover until April in 2013 on the sedimentation. A rapid warming in only 9 days starting on April 9th from -0,3 C° to 15,2 C° resulted in fast ice break-up and a short but intensive lake mixing. In consequence of this short mixing period a strong algal bloom especially of Fragilaria and Crysophycea commenced in April and had its maximum in May. This bloom further induced biogenic

  10. Salinity and hydrology of closed lakes

    USGS Publications Warehouse

    Langbein, Walter Basil

    1961-01-01

    Lakes without outlets, called closed lakes, are exclusively features of the arid and semiarid zones where annual evaporation exceeds rainfall. The number of closed lakes increases with aridity, so there are relatively few perennial closed lakes, but "dry" lakes that rarely contain water are numerous.Closed lakes fluctuate in level to a much greater degree than the open lakes of the humid zone, because variations in inflow can be compensated only by changes in surface area. Since the variability of inflow increases with aridity, it is possible to derive an approximate relationship for the coefficient of variation of lake area in terms of data on rates of evaporation, lake area, lake depth, and drainage area.The salinity of closed lakes is highly variable, ranging from less than 1 percent to over 25 percent by weight of salts. Some evidence suggests that the tonnage of salts in a lake solution is substantially less than the total input of salts into the lake over the period of existence of the closed lake. This evidence suggests further that the salts in a lake solution represent a kind of long-term balance between factors of gain and loss of salts from the solution.Possible mechanisms for the loss of salts dissolved in the lake include deposition in marginal bays, entrapment in sediments, and removal by wind. Transport of salt from the lake surface in wind spray is also a contributing, but seemingly not major, factor.The hypothesis of a long-term balance between input to and losses from the lake solution is checked by deriving a formula for the equilibrium concentration and comparing the results with the salinity data. The results indicate that the reported salinities seemingly can be explained in terms of their geometric properties and hydrologic environment.The time for accumulation of salts in the lake solution the ratio between mass of salts in the solution and the annual input may also be estimated from the geometric and hydrologic factors, in the absence of

  11. Crater Lake revealed

    USGS Publications Warehouse

    Ramsey, David W.; Dartnell, Peter; Bacon, Charles R.; Robinson, Joel E.; Gardner, James V.

    2003-01-01

    Around 500,000 people each year visit Crater Lake National Park in the Cascade Range of southern Oregon. Volcanic peaks, evergreen forests, and Crater Lake’s incredibly blue water are the park’s main attractions. Crater Lake partially fills the caldera that formed approximately 7,700 years ago by the eruption and subsequent collapse of a 12,000-foot volcano called Mount Mazama. The caldera-forming or climactic eruption of Mount Mazama drastically changed the landscape all around the volcano and spread a blanket of volcanic ash at least as far away as southern Canada.Prior to the climactic event, Mount Mazama had a 400,000 year history of cone building activity like that of other Cascade volcanoes such as Mount Shasta. Since the climactic eruption, there have been several less violent, smaller postcaldera eruptions within the caldera itself. However, relatively little was known about the specifics of these eruptions because their products were obscured beneath Crater Lake’s surface. As the Crater Lake region is still potentially volcanically active, understanding past eruptive events is important to understanding future eruptions, which could threaten facilities and people at Crater Lake National Park and the major transportation corridor east of the Cascades.Recently, the lake bottom was mapped with a high-resolution multibeam echo sounder. The new bathymetric survey provides a 2m/pixel view of the lake floor from its deepest basins virtually to the shoreline. Using Geographic Information Systems (GIS) applications, the bathymetry data can be visualized and analyzed to shed light on the geology, geomorphology, and geologic history of Crater Lake.

  12. Lake Sarez, Tajikistan

    NASA Technical Reports Server (NTRS)

    2002-01-01

    Lake Sarez (top), deep in the Pamir mountains of Tajikistan, was created 90 years ago when a strong earthquake triggered a massive landslide that, in turn, became a huge dam along the Murghob River, now called the Usoi Dam. The resulting lake is perched above surrounding drainages at an elevation greater than 3000m, and is part of the watershed that drains the towering Akademi Nauk Range (see the regional image, lower). The lake is 61 km long and as deep as 500 m, and holds an estimated 17 cubic km of water. The area experiences considerable seismic activity, and scientists fear that part of the right bank may slump into the lake, creating a huge wave that will top over and possibly breach the natural dam. Such a wave would create a catastrophic flood downstream along the Bartang, Panj and Amu Darya Rivers, perhaps reaching all the way to the Aral Sea. Currently, central Asian governments, as well as the World Bank and the UN are monitoring the dam closely, and have proposed gradually lowering the lake level as a preventive measure. More information about the lake is available at the following web sites: Lake Sarez Study group, UN Report, Reliefweb Digital photograph numbers ISS002-E-7771 and ISS002-E-7479 were taken in the spring of 2001 from Space Station Alpha and are provided by the Earth Sciences and Image Analysis Laboratory at Johnson Space Center. Additional images taken by astronauts and cosmonauts can be viewed at the NASA-JSC Gateway to Astronaut Photography of Earth.

  13. ATMOSPHERIC MERCURY DEPOSITION TO LAKE MICHIGAN DURING THE LAKE MICHIGAN MASS BALANCE STUDY

    EPA Science Inventory

    Wet and dry mercury (Hg) deposition were calculated to Lake Michigan using a hybrid receptor modeling framework. The model utilized mercury monitoring data collected during the Lake Michigan Mass Balance Study and the Atmospheric Exchange Over Lakes and Oceans Study together w...

  14. The Great Lakes' regional climate regimes

    NASA Astrophysics Data System (ADS)

    Sugiyama, Noriyuki

    For the last couple of decades, the Great Lakes have undergone rapid surface warming. In particular, the magnitude of the summer surface-warming trends of the Great Lakes have been much greater than those of surrounding land (Austin and Colman, 2007). Among the Great Lakes, the deepest Lake Superior exhibited the strongest warming trend in its annual, as well as summer surface water temperature. We find that many aspects of this behavior can be explained in terms of the tendency of deep lakes to exhibit multiple regimes characterized, under the same seasonally varying forcing, by the warmer and colder seasonal cycles exhibiting different amounts of wintertime lake-ice cover and corresponding changes in the summertime lake-surface temperatures. In this thesis, we address the problem of the Great Lakes' warming using one-dimensional lake modeling to interpret diverse observations of the recent lake behavior. (Abstract shortened by ProQuest.).

  15. Characterization of lake water and ground water movement in the littoral zone of Williams Lake, a closed-basin lake in North central Minnesota

    USGS Publications Warehouse

    Schuster, P.F.; Reddy, M.M.; LaBaugh, J.W.; Parkhurst, R.S.; Rosenberry, D.O.; Winter, T.C.; Antweiler, Ronald C.; Dean, W.E.

    2003-01-01

    Williams Lake, Minnesota is a closed-basin lake that is a flow-through system with respect to ground water. Ground-water input represents half of the annual water input and most of the chemical input to the lake. Chemical budgets indicate that the lake is a sink for calcium, yet surficial sediments contain little calcium carbonate. Sediment pore-water samplers (peepers) were used to characterize solute fluxes at the lake-water-ground-water interface in the littoral zone and resolve the apparent disparity between the chemical budget and sediment data. Pore-water depth profiles of the stable isotopes ??18O and ??2H were non-linear where ground water seeped into the lake, with a sharp transition from lake-water values to ground-water values in the top 10 cm of sediment. These data indicate that advective inflow to the lake is the primary mechanism for solute flux from ground water. Linear interstitial velocities determined from ??2H profiles (316 to 528 cm/yr) were consistent with velocities determined independently from water budget data and sediment porosity (366 cm/yr). Stable isotope profiles were generally linear where water flowed out of the lake into ground water. However, calcium profiles were not linear in the same area and varied in response to input of calcium carbonate from the littoral zone and subsequent dissolution. The comparison of pore-water calcium profiles to pore-water stable isotope profiles indicate calcium is not conservative. Based on the previous understanding that 40-50 % of the calcium in Williams Lake is retained, the pore-water profiles indicate aquatic plants in the littoral zone are recycling the retained portion of calcium. The difference between the pore-water depth profiles of calcium and ??18O and ??2H demonstrate the importance of using stable isotopes to evaluate flow direction and source through the lake-water-ground-water interface and evaluate mechanisms controlling the chemical balance of lakes. Published in 2003 by John Wiley

  16. LakeVOC; A Deterministic Model to Estimate Volatile Organic Compound Concentrations in Reservoirs and Lakes

    USGS Publications Warehouse

    Bender, David A.; Asher, William E.; Zogorski, John S.

    2003-01-01

    This report documents LakeVOC, a model to estimate volatile organic compound (VOC) concentrations in lakes and reservoirs. LakeVOC represents the lake or reservoir as a two-layer system and estimates VOC concentrations in both the epilimnion and hypolimnion. The air-water flux of a VOC is characterized in LakeVOC in terms of the two-film model of air-water exchange. LakeVOC solves the system of coupled differential equations for the VOC concentration in the epilimnion, the VOC concentration in the hypolimnion, the total mass of the VOC in the lake, the volume of the epilimnion, and the volume of the hypolimnion. A series of nine simulations were conducted to verify LakeVOC representation of mixing, dilution, and gas exchange characteristics in a hypothetical lake, and two additional estimates of lake volume and MTBE concentrations were done in an actual reservoir under environmental conditions. These 11 simulations showed that LakeVOC correctly handled mixing, dilution, and gas exchange. The model also adequately estimated VOC concentrations within the epilimnion in an actual reservoir with daily input parameters. As the parameter-input time scale increased (from daily to weekly to monthly, for example), the differences between the measured-averaged concentrations and the model-estimated concentrations generally increased, especially for the hypolimnion. This may be because as the time scale is increased from daily to weekly to monthly, the averaging of model inputs may cause a loss of detail in the model estimates.

  17. Is Lake Chabot Eutrophic?

    NASA Astrophysics Data System (ADS)

    Pellegrini, K.; Logan, J.; Esterlis, P.; Lew, A.; Nguyen, M.

    2013-12-01

    Introduction/Abstract: Lake Chabot is an integral part of the East Bay watershed that provides habitats for animals and recreation for humans year-round. Lake Chabot has been in danger of eutrophication due to excessive dumping of phosphorous and nitrogen into the water from the fertilizers of nearby golf courses and neighboring houses. If the lake turned out to be eutrophified, it could seriously impact what is currently the standby emergency water supply for many Castro Valley residents. Eutrophication is the excessive richness of nutrients such as nitrogen and phosphorus in a lake, usually as a result of runoff. This buildup of nutrients causes algal blooms. The algae uses up most of the oxygen in the water, and when it dies, it causes the lake to hypoxify. The fish in the lake can't breathe, and consequently suffocate. Other oxygen-dependant aquatic creatures die off as well. Needless to say, the eutrophication of a lake is bad news for the wildlife that lives in or around it. The level of eutrophication in our area in Northern California tends to increase during the late spring/early summer months, so our crew went out and took samples of Lake Chabot on June 2. We focused on the area of the lake where the water enters, known on the map as Honker Bay. We also took readings a ways down in deeper water for comparison's sake. Visually, the lake looked in bad shape. The water was a murky green that glimmered with particulate matter that swirled around the boat as we went by. In the Honker Bay region where we focused our testing, there were reeds bathed in algae that coated the surface of the lake in thick, swirling patterns. Surprisingly enough, however, our test results didn't reveal any extreme levels of phosphorous or nitrogen. They were slightly higher than usual, but not by any significant amount. The levels we found were high enough to stimulate plant and algae growth and promote eutrophication, but not enough to do any severe damage. After a briefing with a

  18. Cooperative science to inform Lake Ontario management: Research from the 2013 Lake Ontario CSMI program

    USGS Publications Warehouse

    Watkins, James M.; Weidel, Brian C.; Fisk, Aaron T.; Rudstam, Lars G.

    2017-01-01

    Since the mid-1970s, successful Lake Ontario management actions including nutrient load and pollution reductions, habitat restoration, and fish stocking have improved Lake Ontario. However, several new obstacles to maintenance and restoration have emerged. This special issue presents management-relevant research from multiple agency surveys in 2011 and 2012 and the 2013 Cooperative Science and Monitoring Initiative (CSMI), that span diverse lake habitats, species, and trophic levels. This research focused on themes of nutrient loading and fate; vertical dynamics of primary and secondary production; fish abundance and behavior; and food web structure. Together these papers identify the status of many of the key drivers of the Lake Ontario ecosystem and contribute to addressing lake-scale questions and management information needs in Lake Ontario and the other Great Lakes and connecting water bodies.

  19. Hydrology of the Reelfoot Lake basin, Obion and Lake counties, northwestern Tennessee

    USGS Publications Warehouse

    Robbins, C.H.

    1985-01-01

    Nine maps describe the following water resources aspects of the Reelfoot Lake watershed: Map 1-Surface water gaging stations, lake level, and locations of observation wells, rainfall stations and National Weather Service rainfall stations; Maps 2 and 3-water level contours, river stage, groundwater movement; Maps 4 and 5-grid blocks simulating constant head on the Mississippi River, Reelfoot Lake, Running Reelfoot Bayou, Reelfoot Creek, and Running Slough; Maps 6 and 7-difference between model calculated and observed water levels; and Maps 8 and 9-line of equal groundwater level increase and approximate lake area at pool elevation. (Lantz-PTT)

  20. Dendrochronology and lakes: using tree-rings of alder to reconstruct lake levels

    NASA Astrophysics Data System (ADS)

    van der Maaten, Ernst; Buras, Allan; Scharnweber, Tobias; Simard, Sonia; Kaiser, Knut; Lorenz, Sebastian; van der Maaten-Theunissen, Marieke; Wilmking, Martin

    2014-05-01

    Climate change is considered a major threat for ecosystems around the world. Assessing its effects is challenging, amongst others, as we are unsure how ecosystems may respond to climate conditions they were not exposed to before. However, increased insight may be obtained by analyzing responses of ecosystems to past climate variability. In this respect, lake ecosystems appear as valuable sentinels, because they provide direct and indirect indicators of change through effects of climate. Lake-level fluctuations of closed catchments, for example, reflect a dynamic water balance, provide detailed insight in past moisture variations, and thereby allow for assessments of effects of anticipated climate change. Up to now, lake-level data are mostly obtained from gauging records and reconstructions from sediments and landforms. However, these records are in many cases only available over relatively short time periods, and, since geoscientific work is highly demanding, lake-level reconstructions are lacking for many regions. Here, we present and discuss an alternative method to reconstruct lake levels, which is based on tree-ring data of black alder (Alnus glutinosa L.). This tree species tolerates permanently waterlogged and temporally flooded conditions (i.e. riparian vegetation), and is often found along lakeshores. As the yearly growth of trees varies depending upon the experienced environmental conditions, annual rings of black alder from lakeshore vegetation likely capture information on variations in water table, and may therefore be used to reconstruct lake levels. Although alder is a relatively short-lived tree species, the frequent use of its' decay-resistant wood in foundations of historical buildings offers the possibility of extending living tree-chronologies back in time for several centuries. In this study, the potential to reconstruct lake-level fluctuations from tree-ring chronologies of black alder is explored for three lake ecosystems in the Mecklenburg