Nuclear reactor I

DOEpatents

Ference, Edward W.; Houtman, John L.; Waldby, Robert N.

1977-01-01

A nuclear reactor, particularly a liquid-metal breeder reactor whose upper internals include provision for channeling the liquid metal flowing from the core-component assemblies to the outlet plenum in vertical paths in direction generally along the direction of the respective assemblies. The metal is channeled by chimneys, each secured to, and extending from, a grid through whose openings the metal emitted by a plurality of core-component assemblies encompassed by the grid flows. To reduce the stresses resulting from structural interaction, or the transmissive of thermal strains due to large temperature differences in the liquid metal emitted from neighboring core-component assemblies, throughout the chimneys and the other components of the upper internals, the grids and the chimneys are supported from the heat plate and the core barrel by support columns (double portal support) which are secured to the head plate at the top and to a member, which supports the grids and is keyed to the core barrel, at the bottom. In addition to being restrained from lateral flow by the chimneys, the liquid metal is also restrained from flowing laterally by a peripheral seal around the top of the core. This seal limits the flow rate of liquid metal, which may be sharply cooled during a scram, to the outlet nozzles. The chimneys and the grids are formed of a highly-refractory, high corrosion-resistant nickel-chromium-iron alloy which can withstand the stresses produced by temperature differences in the liquid metal. The chimneys are supported by pairs of plates, each pair held together by hollow stubs coaxial with, and encircling, the chimneys. The plates and stubs are a welded structure but, in the interest of economy, are composed of stainless steel which is not weld compatible with the refractory metal. The chimneys and stubs are secured together by shells of another nickel-chromium-iron alloy which is weld compatible with, and is welded to, the stubs and has about the same coefficient of expansion as the highly-refractory, high corrosion-resistant alloy.

The use of laterally vectored thrust to counter thrust asymmetry in a tactical jet aircraft

NASA Technical Reports Server (NTRS)

1983-01-01

A nonlinear, six degree-of-freedom flight simulator for a twin engine tactical jet was built on a hybrid computer to investigate lateral vectoring of the remaining thrust component for the case of a single engine failure at low dynamic pressures. Aircraft control was provided by an automatic controller rather than a pilot, and thrust vector control was provided by an open-loop controller that deflected a vane (located on the periphery of each exhaust jet and normally streamlined for noninterference with the flow). Lateral thrust vectoring decreased peak values of lateral control deflections, eliminated the requirement for steady-state lateral aerodynamic control deflections, and decreased the amount of altitude lost for a single engine failure.

Analysis of water surface and flow distribution for the design flood at a proposed highway crossing of the Sabine River near Tatum, Texas

USGS Publications Warehouse

Gilbert, J.J.; Myers, D.R.

1989-01-01

The simulations of the proposed and alternate designs indicate a lateral component of the water-surface slope at the embankment. Redistribution of flow across the floodplain also is indicated in both simulations. Some of the differences in the response between the two designs are affected by geometric features of the floodplain other than the embankment-opening geometry.

Dual processing of visual rotation for bipedal stance control.

PubMed

Day, Brian L; Muller, Timothy; Offord, Joanna; Di Giulio, Irene

2016-10-01

When standing, the gain of the body-movement response to a sinusoidally moving visual scene has been shown to get smaller with faster stimuli, possibly through changes in the apportioning of visual flow to self-motion or environment motion. We investigated whether visual-flow speed similarly influences the postural response to a discrete, unidirectional rotation of the visual scene in the frontal plane. Contrary to expectation, the evoked postural response consisted of two sequential components with opposite relationships to visual motion speed. With faster visual rotation the early component became smaller, not through a change in gain but by changes in its temporal structure, while the later component grew larger. We propose that the early component arises from the balance control system minimising apparent self-motion, while the later component stems from the postural system realigning the body with gravity. The source of visual motion is inherently ambiguous such that movement of objects in the environment can evoke self-motion illusions and postural adjustments. Theoretically, the brain can mitigate this problem by combining visual signals with other types of information. A Bayesian model that achieves this was previously proposed and predicts a decreasing gain of postural response with increasing visual motion speed. Here we test this prediction for discrete, unidirectional, full-field visual rotations in the frontal plane of standing subjects. The speed (0.75-48 deg s(-1) ) and direction of visual rotation was pseudo-randomly varied and mediolateral responses were measured from displacements of the trunk and horizontal ground reaction forces. The behaviour evoked by this visual rotation was more complex than has hitherto been reported, consisting broadly of two consecutive components with respective latencies of ∼190 ms and >0.7 s. Both components were sensitive to visual rotation speed, but with diametrically opposite relationships. Thus, the early component decreased with faster visual rotation, while the later component increased. Furthermore, the decrease in size of the early component was not achieved by a simple attenuation of gain, but by a change in its temporal structure. We conclude that the two components represent expressions of different motor functions, both pertinent to the control of bipedal stance. We propose that the early response stems from the balance control system attempting to minimise unintended body motion, while the later response arises from the postural control system attempting to align the body with gravity. © 2016 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society.

The generation of monoclonal antibodies and their use in rapid diagnostic tests

USDA-ARS?s Scientific Manuscript database

Antibodies are the most important component of an immunoassay. In these proceedings we outline novel methods used to generate and select monoclonal antibodies that meet performance criteria for use in rapid lateral flow and microfluidic immunoassay tests for the detection of agricultural pathogens ...

Unsteady density-current equations for highly curved terrain

NASA Technical Reports Server (NTRS)

Sivakumaran, N. S.; Dressler, R. F.

1989-01-01

New nonlinear partial differential equations containing terrain curvature and its rate of change are derived that describe the flow of an atmospheric density current. Unlike the classical hydraulic-type equations for density currents, the new equations are valid for two-dimensional, gradually varied flow over highly curved terrain, hence suitable for computing unsteady (or steady) flows over arbitrary mountain/valley profiles. The model assumes the atmosphere above the density current exerts a known arbitrary variable pressure upon the unknown interface. Later this is specialized to the varying hydrostatic pressure of the atmosphere above. The new equations yield the variable velocity distribution, the interface position, and the pressure distribution that contains a centrifugal component, often significantly larger than its hydrostatic component. These partial differential equations are hyperbolic, and the characteristic equations and characteristic directions are derived. Using these to form a characteristic mesh, a hypothetical unsteady curved-flow problem is calculated, not based upon observed data, merely as an example to illustrate the simplicity of their application to unsteady flows over mountains.

Erosional furrows formed during the lateral blast at Mount St. Helens, May 18, 1980

USGS Publications Warehouse

Kieffer, S.W.; Sturtevant, B.

1988-01-01

Nearly horizontal, quasi-periodic erosional features of 7-m average transverse wavelength and of order 100-m length occur in scattered locations from 3.5 to 9 km from the crater at Mount St. Helens under deposits of the lateral blast of May 18, 1980. We attribute the erosional features to scouring by longitudinal vortices resulting from flow instabilities induced by complex topography, namely, by streamline curvature in regions of reattachment downstream of sheltered regions, and by the cross-flow component of flow subparallel to ridge crests. The diameter of the vortices and their transverse spacing, inferred from the distance between furrows, are taken to be of the order of the boundary layer thickness. The inferred boundary layer thickness (???14 m at 9 km from the source of the blast) is consistent with the running length from the mountain to the furrow locations. The orientation of furrows induced by the cross-flow instability can be used to measure the upwash angle and estimate the flow Mach number: at the central ridge of Spirit Lake the Mach number is inferred to have been about 2.5, and the flow velocity approximately 235 m/s. -from Authors

Preliminary interpretation of thermal data from the Nevada Test Site

USGS Publications Warehouse

Sass, John Harvey; Lachenbruch, Arthur H.

1982-01-01

Analysis of data from 60 wells in and around the Nevada Test Site, including 16 in the Yucca Mountain area, indicates a thermal regime characterized by large vertical and lateral gradients in heat flow. Estimates of heat flow indicate considerable variation on both regional and local scales. The variations are attributable primarily to hydrologic processes involving interbasin flow with a vertical component of (seepage) velocity (volume flux) of a few mm/yr. Apart from indicating a general downward movement of water at a few mm/yr, the results from Yucca Mountain are as yet inconclusive.

Modeling lateral circulation and its influence on the along-channel flow in a branched estuary

NASA Astrophysics Data System (ADS)

Zhu, Lei; He, Qing; Shen, Jian

2018-02-01

A numerical modeling study of the influence of the lateral flow on the estuarine exchange flow was conducted in the north passage of the Changjiang estuary. The lateral flows show substantial variabilities within a flood-ebb tidal cycle. The strong lateral flow occurring during flood tide is caused primarily by the unique cross-shoal flow that induces a strong northward (looking upstream) barotropic force near the surface and advects saltier water toward the northern part of the channel, resulting in a southward baroclinic force caused by the lateral density gradient. Thus, a two-layer structure of lateral flows is produced during the flood tide. The lateral flows are vigorous near the flood slack and the magnitude can exceed that of the along-channel tidal flow during that period. The strong vertical shear of the lateral flows and the salinity gradient in lateral direction generate lateral tidal straining, which are out of phase with the along-channel tidal straining. Consequently, stratification is enhanced at the early stage of the ebb tide. In contrast, strong along-channel straining is apparent during the late ebb tide. The vertical mixing disrupts the vertical density gradient, thus suppressing stratification. The impact of lateral straining on stratification during spring tide is more pronounced than that of along-channel straining during late flood and early ebb tides. The momentum balance along the estuary suggests that lateral flow can augment the residual exchange flow. The advection of lateral flows brings low-energy water from the shoal to the deep channel during the flood tide, whereas the energetic water is moved to the shoal via lateral advection during the ebb tide. The impact of lateral flow on estuarine circulation of this multiple-channel estuary is different from single-channel estuary. A model simulation by blocking the cross-shoal flow shows that the magnitudes of lateral flows and tidal straining are reduced. Moreover, the reduced lateral tidal straining results in a decrease in vertical stratification from the late flood to early ebb tides during the spring tide. By contrast, the along-channel tidal straining becomes dominant. The model results illustrate the important dynamic linkage between lateral flows and estuarine dynamics in the Changjiang estuary.

The interaction of a magnetic cloud with the Earth - Ionospheric convection in the Northern and Southern Hemispheres for a wide range of quasi-steady interplanetary magnetic field conditions

NASA Technical Reports Server (NTRS)

Freeman, M. P.; Farrugia, C. J.; Burlaga, L. F.; Hairston, M. R.; Greenspan, M. E.; Ruohoniemi, J. M.; Lepping, R. P.

1993-01-01

Observations are presented of the ionospheric convection in cross sections of the polar cap and auroral zone as part of the study of the interaction of the Earth's magnetosphere with the magnetic cloud of January 13-15, 1988. For strongly northward IMF, the convection in the Southern Hemisphere is characterized by a two-cell convection pattern comfined to high latitudes with sunward flow over the pole. The strength of the flows is comparable to that later seen under southward IMF. Superimposed on this convection pattern there are clear dawn-dusk asymmetries associated with a one-cell convection component whose sense depends on the polarity of the magnetic cloud's large east-west magnetic field component. When the cloud's magnetic field turns southward, the convection is characterized by a two-cell pattern extending to lower latitude with antisunward flow over the pole. There is no evident interhemispheric difference in the structure and strength of the convection. Superimposed dawn-dusk asymmetries in the flow patterns are observed which are only in part attributable to the east-west component of the magnetic field.

Lateral flow strip assay

DOEpatents

Miles, Robin R [Danville, CA; Benett, William J [Livermore, CA; Coleman, Matthew A [Oakland, CA; Pearson, Francesca S [Livermore, CA; Nasarabadi, Shanavaz L [Livermore, CA

2011-03-08

A lateral flow strip assay apparatus comprising a housing; a lateral flow strip in the housing, the lateral flow strip having a receiving portion; a sample collection unit; and a reagent reservoir. Saliva and/or buccal cells are collected from an individual using the sample collection unit. The sample collection unit is immersed in the reagent reservoir. The tip of the lateral flow strip is immersed in the reservoir and the reagent/sample mixture wicks up into the lateral flow strip to perform the assay.

Evidence for the Activation of Shallow Preferential Flow Paths in a Tropical Panama Watershed Using Germanium and Silicon

NASA Astrophysics Data System (ADS)

Gardner, Christopher B.; Litt, Guy F.; Lyons, W. Berry; Ogden, Fred L.

2017-10-01

In humid tropical watersheds, the hydrologic flow paths taken by rain event waters and how they interact with groundwater and soil matrix water to form streamflow are poorly understood. Preferential flow paths (PFPs) confound storm infiltration processes, especially in the humid tropics where PFPs are common. This work applies germanium (Ge) and silicon (Si) as natural flow path tracers in conjunction with water stable isotopes and electrical conductivity to examine the rapid delivery of shallow soil water, the activation of PFPs, and event water partitioning in an experimental catchment in central Panama. We employed a three-component mixing model for hydrograph separation using the following end-member waters: (i) base flow (high [Si], low [Ge], and low Ge/Si ratio), (ii) dilute canopy throughfall (low [Si] and low [Ge]), and (iii) shallow (<15 cm) soil matrix water (low [Si], high [Ge], and high Ge/Si ratio). These three end-members bounded all observed Ge/Si streamflow ratios. During small rain events (<˜24 mm), base flow and dilute canopy throughfall components dominated stormflow. During larger precipitation events (>˜35 mm), we detected the third shallow soil water component with an elevated [Ge] and Ge/Si ratio. This component reached its maximum during the hydrograph's receding limb coincident with the maximum event fraction, and increased proportionally to the total storm rainfall exceeding ˜35 mm. Only shallow (<15 cm) soil matrix water exhibited elevated Ge concentrations and high Ge/Si ratios. This third component represents rapidly delivered soil matrix water combined with shallow lateral PFP activation through which event waters interact with soil minerals.

Studies of interactions of a propagating shock wave with decaying grid turbulence: velocity and vorticity fields

NASA Astrophysics Data System (ADS)

Agui, Juan H.; Briassulis, George; Andreopoulos, Yiannis

2005-02-01

The unsteady interaction of a moving shock wave with nearly homogeneous and isotropic decaying compressible turbulence has been studied experimentally in a large-scale shock tube facility. Rectangular grids of various mesh sizes were used to generate turbulence with Reynolds numbers based on Taylor's microscale ranging from 260 to 1300. The interaction has been investigated by measuring the three-dimensional velocity and vorticity vectors, the full velocity gradient and rate-of-strain tensors with instrumentation of high temporal and spatial resolution. This allowed estimates of dilatation, compressible dissipation and dilatational stretching to be obtained. The time-dependent signals of enstrophy, vortex stretching/tilting vector and dilatational stretching vector were found to exhibit a rather strong intermittent behaviour which is characterized by high-amplitude bursts with values up to 8 times their r.m.s. within periods of less violent and longer lived events. Several of these bursts are evident in all the signals, suggesting the existence of a dynamical flow phenomenon as a common cause. Fluctuations of all velocity gradients in the longitudinal direction are amplified significantly downstream of the interaction. Fluctuations of the velocity gradients in the lateral directions show no change or a minor reduction through the interaction. Root mean square values of the lateral vorticity components indicate a 25% amplification on average, which appears to be very weakly dependent on the shock strength. The transmission of the longitudinal vorticity fluctuations through the shock appears to be less affected by the interaction than the fluctuations of the lateral components. Non-dissipative vortex tubes and irrotational dissipative motions are more intense in the region downstream of the shock. There is also a significant increase in the number of events with intense rotational and dissipative motions. Integral length scales and Taylor's microscales were reduced after the interaction with the shock in all investigated flow cases. The integral length scales in the lateral direction increase at low Mach numbers and decrease during strong interactions. It appears that in the weakest of the present interactions, turbulent eddies are compressed drastically in the longitudinal direction while their extent in the normal direction remains relatively the same. As the shock strength increases the lateral integral length scales increase while the longitudinal ones decrease. At the strongest interaction of the present flow cases turbulent eddies are compressed in both directions. However, even at the highest Mach number the issue is more complicated since amplification of the lateral scales has been observed in flows with fine grids. Thus the outcome of the interaction strongly depends on the initial conditions.

Understanding Friction Stir Welding

NASA Technical Reports Server (NTRS)

Nunes, A. C., Jr.

2018-01-01

This Technical Memorandum explains the friction stir welding process in terms of two basic concepts: the concentration of deformation in a shear surface enveloping the tool and the composition of the overall plastic flow field around the tool from simple flow field components. It is demonstrated how weld structure may be understood and torque, drag, and lateral tool forces may be estimated using these concepts. Some discrepancies between computations and accompanying empirical data are discussed in the text. This work is intended to be helpful to engineers in diagnosing problems and advancing technology.

Regional dolomitization of the Waulsortian limestone in southeastern Ireland: Evidence of large-scale fluid flow driven by the Hercynian orogeny

NASA Astrophysics Data System (ADS)

Hitzman, M. W.; Allan, J. R.; Beaty, D. W.

1998-06-01

The Lower Carboniferous Waulsortian limestone has been affected by late diagenetic, regional burial dolomitization over an area of ˜7000 km2 extending 170 km northeastward from the Hercynian front. The dolostone is composed of two components: (1) a very fine crystalline replacive component, and (2) a coarse-crystalline, baroque component that fills vuggy porosity developed within the replacive dolostone. Fluid inclusions within the coarse-crystalline component of the regional dolostone indicate that the dolomitizing solutions were warm (˜100 °C) and moderately saline (10 13 wt% NaCl equivalent). The replacive dolomite displays systematic decreases in crystal size and degree of xenotopic texture northward. Both components of the regional dolostone display a regular northward increase in mean δ18O values; the replacive dolomite has 87Sr/86Sr values that decrease northward. These laterally variable characteristics suggest that regional dolomitization resulted from large-scale, north-directed fluid flow of heated brines developed in response to topographic uplift associated with collapse of the continental margin south of Ireland during the Hercynian (Variscan) orogeny.

Unstructured Grid Euler Method Assessment for Longitudinal and Lateral/Directional Aerodynamic Performance Analysis of the HSR Technology Concept Airplane at Supersonic Cruise Speed

NASA Technical Reports Server (NTRS)

Ghaffari, Farhad

1999-01-01

Unstructured grid Euler computations, performed at supersonic cruise speed, are presented for a High Speed Civil Transport (HSCT) configuration, designated as the Technology Concept Airplane (TCA) within the High Speed Research (HSR) Program. The numerical results are obtained for the complete TCA cruise configuration which includes the wing, fuselage, empennage, diverters, and flow through nacelles at M (sub infinity) = 2.4 for a range of angles-of-attack and sideslip. Although all the present computations are performed for the complete TCA configuration, appropriate assumptions derived from the fundamental supersonic aerodynamic principles have been made to extract aerodynamic predictions to complement the experimental data obtained from a 1.675%-scaled truncated (aft fuselage/empennage components removed) TCA model. The validity of the computational results, derived from the latter assumptions, are thoroughly addressed and discussed in detail. The computed surface and off-surface flow characteristics are analyzed and the pressure coefficient contours on the wing lower surface are shown to correlate reasonably well with the available pressure sensitive paint results, particularly, for the complex flow structures around the nacelles. The predicted longitudinal and lateral/directional performance characteristics for the truncated TCA configuration are shown to correlate very well with the corresponding wind-tunnel data across the examined range of angles-of-attack and sideslip. The complementary computational results for the longitudinal and lateral/directional performance characteristics for the complete TCA configuration are also presented along with the aerodynamic effects due to empennage components. Results are also presented to assess the computational method performance, solution sensitivity to grid refinement, and solution convergence characteristics.

Interaction of weakly compressible isotropic turbulence with planar expansion waves: Flow anisotropy and vorticity alignment

NASA Astrophysics Data System (ADS)

Xanthos, Savvas; Gong, Minwei; Andreopoulos, Yiannis

2010-01-01

Further analysis of the experimental data of the velocity gradient tensor first published by Xanthos et al. [J. Fluid Mech. 584, 301 (2007)] has been carried out and new results are reported here to provide additional insights on the effects of expansion waves interacting with isotropic turbulence. The flow field was generated by the reflection of an incoming shock wave at the open end of a large scale shock tube facility which interacted with the induced flow behind the incident shock wave which passed through a turbulence generating grid. In the present configuration the interaction is free from streamline curvature effects, which cause additional effects on turbulence. The strength of the applied expansive straining was 240 s-1. Rectangular pattern grids of different mesh sizes were used to generate isotropic and homogeneous turbulence with turbulent Reynolds number Reλ based on Taylor's microscale between 450 and 488. Lateral vorticity fluctuations and fluctuations of enstrophy and all stretching vector components are drastically reduced during the interaction. Residual attenuation in the postinteraction flow field was found only in the lateral vorticity fluctuations and in the longitudinal stretching term S11Ω1. Helicity and the helicity angle were computed from the data and the orientation angle of the vorticity vector in reference to the velocity vector was determined. Large fluctuations of the helicity angle were observed which extend from 0° to 180° with most probable values close to 30° and 130° and a mean value of 85°. Rotational dissipation rate was found to be high at these angles. The time-dependent signals of enstrophy, vortex stretching/tilting vector, and dissipation rate were found to exhibit a rather strong intermittent behavior which is characterized by high amplitude bursts followed by low level activities. It was found that the observed strong dissipative events are mostly associated with strong activities in the longitudinal stretching S11Ω1 rather than with events in the lateral components.

Improving Long-term Post-wildfire hydrologic simulations using ParFlow

NASA Astrophysics Data System (ADS)

Lopez, S. R.; Kinoshita, A. M.

2015-12-01

Wildfires alter the natural hydrologic processes within a watershed. After vegetation is burned, the combustion of organic material and debris settles into the soil creating a hydrophobic layer beneath the soil surface with varying degree of thickness and depth. Vegetation regrowth rates vary as a function of radiative exposure, burn severity, and precipitation patterns. Hydrologic models used by the Burned Area Emergency Response (BAER) teams use input data and model calibration constraints that are generally either one-dimensional, empirically-based models, or two-dimensional, conceptually-based models with lumped parameter distributions. These models estimate runoff measurements at the watershed outlet; however, do not provide a distributed hydrologic simulation at each point within the watershed. This work uses ParFlow, a three-dimensional, distributed hydrologic model to (1) correlate burn severity with hydrophobicity, (2) evaluate vegetation recovery rate on water components, and (3) improve flood prediction for managers to help with resource allocation and management operations in burned watersheds. ParFlow is applied to Devil Canyon (43 km2) in San Bernardino, California, which was 97% burned in the 2003 Old Fire. The model set-up uses a 30m-cell size resolution over a 6.7 km by 6.4 km lateral extent. The subsurface reaches 30 m and is assigned a variable cell thickness. Variable subsurface thickness allows users to explicitly consider the degree of recovery throughout the stages of regrowth. Burn severity maps from remotely sensed imagery are used to assign initial hydrophobic layer parameters and thickness. Vegetation regrowth is represented with satellite an Enhanced Vegetation Index. Pre and post-fire hydrologic response is evaluated using runoff measurements at the watershed outlet, and using water component (overland flow, lateral flow, baseflow) measurements.

Chemical provinces and dynamic melting of the NE Atlantic mantle

NASA Astrophysics Data System (ADS)

Tronnes, R. G.

2009-12-01

Low-degree melting of fertile parts of the NE Atlantic mantle yields primitive alkaline basalts in the Icelandic off-rift zones and at Jan Mayen. Olivine tholeiites in the Icelandic rift zones and oceanic spreading ridges are formed by protracted decompressional melting. The V-shaped ridges SW and NE of Iceland indicate that rising, hot material is supplied by a pulsating plume and deflected laterally for distances of about 1000 km from Iceland (Jones et al. GGG 2002; Breivik et al. JGR 2006). Plume material deflected along the rift zones and spreading ridges undergoes mixing with the ambient asthenosphere and extensive melting at shallow level, whereas material deflected in other directions may flow laterally at deeper levels and remain largely unmelted and fertile. A recent investigation of a suite of primitive off-rift basalts from Iceland and Jan Mayen (Debaille et al., 2009, GCA) demonstrated an important source contribution from subcontinental lithospheric mantle (SCLM). Available data on the primitive off-rift basalts and tholeiitic basalts from Iceland and the NE Atlantic ridges indicates the existence of three main composite mantle components, characterized by the following relative isotope ratios (H: high, I: intermediate and L: low ratio) for 87/86Sr, 143/144Nd, 206/204Pb, 187/188Os and 3/4He, respectively: 1. Iceland plume with depleted lower mantle mixed with recycled oceanic crust: I, I, H, H, H 2. Strongly depleted and later re-enriched SCLM: H, L, I, L, L 3. Depleted asthenosphere: L, H, L, I, L The two first composite components contain enriched and depleted subcomponents with distinct isotope signatures. The isotope ratio variations between the fertile components are larger than between the refractory components. The 3/4He ratio, however, is much higher in the depleted plume component than in the depleted SCLM and asthenospheric components. The old SCLM material could in principle be recycled and embedded in the lower mantle and supplied to the melting zone by the Iceland plume. However, a regional isotopic variation pattern indicates that this material originated from the nearby continents and became partially delaminated and embedded in the upper mantle during the recent continental rifting and separation of Greenland the Jan Mayen Ridge and of Greenland and Spitsbergen. The influence of SCLM is most clearly recognized north of central Iceland, in the Northern Rift Zone, along the Kolbeinsey, Mohns, Knipovich and Gakkel Ridges, and especially at Jan Mayen and along the westernmost Gakkel Ridge close to the Yermak Plateau (Goldstein et al. 2008, Nature). The SCLM-signal is weaker for Snæfellsnes, the Mid-Icelandic Belt and the Western and Eastern Rift Zones, and weakest for Vestmannaeyjar, the Southern Volcanic Flank Zone, the Reykjanes Peninsula and the Reykjanes Ridge. The regional geochemical patterns have interesting implications for the probable interaction between lateral plume flow, ridge-focussed asthenospheric flow and delaminated patches of SCLM.

Component build-up method for engineering analysis of missiles at low-to-high angles of attack

NASA Technical Reports Server (NTRS)

Hemsch, Michael J.

1992-01-01

Methods are presented for estimating the component build-up terms, with the exception of zero-lift drag, for missile airframes in steady flow and at arbitrary angles of attack and bank. The underlying and unifying bases of all these efforts are slender-body theory and its nonlinear extensions through the equivalent angle-of-attack concept. Emphasis is placed on the forces and moments which act on each of the fins, so that control cross-coupling effects as well as longitudinal and lateral-directional effects can be determined.

Smartphone-Based Dual-Modality Imaging System for Quantitative Detection of Color or Fluorescent Lateral Flow Immunochromatographic Strips

NASA Astrophysics Data System (ADS)

Hou, Yafei; Wang, Kan; Xiao, Kun; Qin, Weijian; Lu, Wenting; Tao, Wei; Cui, Daxiang

2017-04-01

Nowadays, lateral flow immunochromatographic assays are increasingly popular as a diagnostic tool for point-of-care (POC) test based on their simplicity, specificity, and sensitivity. Hence, quantitative detection and pluralistic popular application are urgently needed in medical examination. In this study, a smartphone-based dual-modality imaging system was developed for quantitative detection of color or fluorescent lateral flow test strips, which can be operated anywhere at any time. In this system, the white and ultra-violet (UV) light of optical device was designed, which was tunable with different strips, and the Sobel operator algorithm was used in the software, which could enhance the identification ability to recognize the test area from the background boundary information. Moreover, this technology based on extraction of the components from RGB format (red, green, and blue) of color strips or only red format of the fluorescent strips can obviously improve the high-signal intensity and sensitivity. Fifty samples were used to evaluate the accuracy of this system, and the ideal detection limit was calculated separately from detection of human chorionic gonadotropin (HCG) and carcinoembryonic antigen (CEA). The results indicated that smartphone-controlled dual-modality imaging system could provide various POC diagnoses, which becomes a potential technology for developing the next-generation of portable system in the near future.

Coherence of simulated atmospheric boundary-layer turbulence

NASA Astrophysics Data System (ADS)

Jiadong, Zeng; Zhiguo, Li; Mingshui, Li

2017-12-01

The coherences in a plane perpendicular to incoming flow are measured in wind tunnel simulations of atmospheric turbulent flow. The measured coherences are compared with analytical expressions tailored to field measurements and with theoretical coherence models which assume homogeneous turbulence and the von Kármán’s spectrum. The comparison indicates that the simulated atmospheric boundary layer flow is approximately horizontally homogeneous turbulence. Based on the above assumption and the systematic analysis of lateral coherence, it can be concluded that the lateral coherences of simulated atmospheric boundary turbulence can be determined accurately using the von Kármán spectrum and the turbulence parameters measured by a few measurement points. The measured results also show that the spatial characteristics of vertical coherences are closely related to the dimensionless parameter {{Δ }}z/({\\bar{z}}0.3{L}ux 0.7). The vertical coherence at two heights can be roughly estimated by the ratio to {{Δ }}z/({\\bar{z}}0.3{L}ux 0.7). The relationship between the phase angles of u-, v- and w-components and the vertical separation distance and the height from the ground is further analyzed. Finally, the roles of the type of land surface roughness, the height from the ground, the turbulence intensity and the integral length scale in lateral and vertical coherences are also discussed in this study.

Spatially explicit simulation of hydrologically controlled carbon and nitrogen cycles and associated feedback mechanisms in a boreal ecosystem in Eastern Canada.

NASA Astrophysics Data System (ADS)

Govind, A.; Chen, J. M.; Margolis, H.

2007-12-01

Current estimates of terrestrial carbon overlook the effects of topographically-driven lateral flow of soil water. We hypothesize that this component, which occur at a landscape or watershed scale have significant influences on the spatial distribution of carbon, due to its large contribution to the local water balance. To this end, we further developed a spatially explicit ecohydrological model, BEPS-TerrainLab V2.0. We simulated the coupled hydrological and carbon cycle processes in a black spruce-moss ecosystem in central Quebec, Canada. The carbon stocks were initialized using a long term carbon cycling model, InTEC, under a climate change and disturbance scenario, the accuracy of which was determined with inventory plot measurements. Further, we simulated and validated several ecosystem indicators such as ET, GPP, NEP, water table, snow depth and soil temperature, using the measurements for two years, 2004 and 2005. After gaining confidence in the model's ability to simulate ecohydrological processes, we tested the influence of lateral water flow on the carbon cycle. We made three hydrological modeling scenarios 1) Explicit, were realistic lateral water routing was considered 2) Implicit where calculations were based on a bucket modeling approach 3) NoFlow, where the lateral water flow was turned off in the model. The results showed that pronounced anomalies exist among the scenarios for the simulated GPP, ET and NEP. In general, Implicit calculation overestimated GPP and underestimated NEP, as opposed to Explicit simulation. NoFlow underestimated GPP and overestimated NEP. The key processes controlling GPP were manifested through stomatal conductance which reduces under conditions of rapid soil saturation ( NoFlow ) or increases in the Implicit case, and, nitrogen availability which affects Vcmax, the maximum carboxylation rate. However, for NEP, the anomalies were attributed to differences in soil carbon pool decomposition, which determine the heterotrophic respiration and the resultant nitrogen mineralization which affects GPP and several other feedback mechanisms. These results suggest that lateral water flow does play a significant role in the terrestrial carbon distribution. Therefore, regional or global scale terrestrial carbon estimates could have significant errors if proper hydrological constrains are not considered for modeling ecological processes due to large topographic variations on the Earth's surface. For more info please visit: http://ajit.govind.googlepages.com/agu2007

Flow of ultra-hot Precambrian orogens and the making of crustal layering in Phanerozoic orogenic plateaux

NASA Astrophysics Data System (ADS)

Chardon, Dominique; Gapais, Denis; Cagnard, Florence; Jayananda, Mudlappa; Peucat, Jean-Jacques

2010-05-01

Reassessment of structural / metamorphic properties of ultra-hot Precambrian orogens and shortening of model weak lithospheres support a syn-convergence flow mode on an orogen scale, with a large component of horizontal finite elongation parallel to the orogen. This orogen-scale flow mode combines distributed shortening, gravity-driven flow, lateral escape, and three-dimensional mass redistribution of buried supracrustal rocks, magmas and migmatites in a thick fluid lower crust. This combination preserves a nearly flat surface and Moho. The upper crust maintains a nearly constant thickness by real-time erosion and near-field clastic sedimentation and by ablation at its base by burial of pop-downs into the lower crust. Steady state regime of these orogens is allowed by activation of an attachment layer that maintains kinematic compatibility between the thin and dominantly plastic upper crust and a thick "water bed" of lower crust. Because very thin lithospheres of orogenic plateaux and Precambrian hot orogens have similar thermomechanical structures, bulk orogenic flow comparable to that governing Precambrian hot orogens should actually operate through today's orogenic plateaux as well. Thus, syn-convergence flow fabrics documented on exposed crustal sections of ancient hot orogens that have not undergone collapse may be used to infer the nature of flow fabrics that are imaged by geophysical techniques beneath orogenic plateaux. We provide a detailed geological perspective on syn-convergence crustal flow in relation to magma emplacement and partial melting on a wide oblique crustal transition of the Neoarchean ultra-hot orogen of Southern India. We document sub-horizontal bulk longitudinal flow of the partially molten lower crust over a protracted period of 60 Ma. Bulk flow results from the interplay of (1) pervasive longitudinal transtensional flow of the partially molten crust, (2) longitudinal coaxial flow on flat fabrics in early plutons, (3) distributed, orogen-normal shortening, (4) emplacement of late prolate shape plutons in the direction of flow, and (5) late, conjugate strike-slip shearing. The macroscopic- to regional scale tectonoplutonic pattern produced by longitudinal flow forms a flat composite anisotropy throughout the lower crust. In the light of GPS data, these results suggest that bulk longitudinal flow accounts for observed deformation of the Tibetan plateau as well as for its seismic structure. This flow mode may be preferred to lateral, east-directed channel flow because it combines both lateral gravity-driven thinning and distributed, orogen-normal shortening of the crust. These results further suggest that lower crustal seismic reflectivity in orogenic belts may not necessarily images fabrics produced by extensional tectonics, as commonly thought, but crustal layering produced by syn-convergence lateral flow.

A lateral electrophoretic flow diagnostic assay

PubMed Central

Lin, Robert; Skandarajah, Arunan; Gerver, Rachel E.; Neira, Hector D.; Fletcher, Daniel A.

2015-01-01

Immunochromatographic assays are a cornerstone tool in disease screening. To complement existing lateral flow assays (based on wicking flow) we introduce a lateral flow format that employs directed electrophoretic transport. The format is termed a “lateral e-flow assay” and is designed to support multiplexed detection using immobilized reaction volumes of capture antigen. To fabricate the lateral e-flow device, we employ mask-based UV photopatterning to selectively immobilize unmodified capture antigen along the microchannel in a barcode-like pattern. The channel-filling polyacrylamide hydrogel incorporates a photoactive moiety (benzophenone) to immobilize capture antigen to the hydrogel without a priori antigen modification. We report a heterogeneous sandwich assay using low-power electrophoresis to drive biospecimen through the capture antigen barcode. Fluorescence barcode readout is collected via a low-resource appropriate imaging system (CellScope). We characterize lateral e-flow assay performance and demonstrate a serum assay for antibodies to the hepatitis C virus (HCV). In a pilot study, the lateral e-flow assay positively identifies HCV+ human sera in 60 min. The lateral e-flow assay provides a flexible format for conducting multiplexed immunoassays relevant to confirmatory diagnosis in near-patient settings. PMID:25608872

Analysis of the NASA/MSFC Airborne Doppler Lidar results from San Gorgonio Pass, California

NASA Technical Reports Server (NTRS)

Cliff, W. C.; Skarda, J. R.; Renne, D. S.; Sandusky, W. F.

1984-01-01

Two days during July of 1981 the NASA/MSFC Airborne Doppler Lidar System (ADLS) was flown aboard the NASA/AMES Convair 990 on the east side of San Gorgonio Pass California, near Palm Springs, to measure and investigate the accelerated atmospheric wind field discharging from the pass. The vertical and horizontal extent of the fast moving atmospheric flow discharging from the San Gorgonio Pass were examined. Conventional ground measurements were also taken during the tests to assist in validating the ADLS results. This particular region is recognized as a high wind resource region and, as such, a knowledge of the horizontal and vertical extent of this flow was of interest for wind energy applications. The statistics of the atmospheric flow field itself as it discharges from the pass and then spreads out over the desert were also of scientific interests. This data provided the first spatial data for ensemble averaging of spatial correlations to compute longitudinal and lateral integral length scales in the longitudinal and lateral directions for both components.

Lateral and subsurface flows impact arctic coastal plain lake water budgets

USGS Publications Warehouse

Koch, Joshua C.

2016-01-01

Arctic thaw lakes are an important source of water for aquatic ecosystems, wildlife, and humans. Many recent studies have observed changes in Arctic surface waters related to climate warming and permafrost thaw; however, explaining the trends and predicting future responses to warming is difficult without a stronger fundamental understanding of Arctic lake water budgets. By measuring and simulating surface and subsurface hydrologic fluxes, this work quantified the water budgets of three lakes with varying levels of seasonal drainage, and tested the hypothesis that lateral and subsurface flows are a major component of the post-snowmelt water budgets. A water budget focused only on post-snowmelt surface water fluxes (stream discharge, precipitation, and evaporation) could not close the budget for two of three lakes, even when uncertainty in input parameters was rigorously considered using a Monte Carlo approach. The water budgets indicated large, positive residuals, consistent with up to 70% of mid-summer inflows entering lakes from lateral fluxes. Lateral inflows and outflows were simulated based on three processes; supra-permafrost subsurface inflows from basin-edge polygonal ground, and exchange between seasonally drained lakes and their drained margins through runoff and evapotranspiration. Measurements and simulations indicate that rapid subsurface flow through highly conductive flowpaths in the polygonal ground can explain the majority of the inflow. Drained lakes were hydrologically connected to marshy areas on the lake margins, receiving water from runoff following precipitation and losing up to 38% of lake efflux to drained margin evapotranspiration. Lateral fluxes can be a major part of Arctic thaw lake water budgets and a major control on summertime lake water levels. Incorporating these dynamics into models will improve our ability to predict lake volume changes, solute fluxes, and habitat availability in the changing Arctic.

Investigation of transonic flow over segmented slotted wind tunnel wall with mass transfer

NASA Technical Reports Server (NTRS)

Bhat, M. K.; Vakili, A. D.; Wu, J. M.

1990-01-01

The flowfield on a segmented multi-slotted wind tunnel wall was studied at transonic speeds by measurements in and near the wall layer using five port cone probes. The slotted wall flowfield was observed to be three-dimensional in nature for a relatively significant distance above the slot. The boundary layer characteristics measured on the single slotted wall were found to be very sensitive to the applied suction through the slot. The perturbation in the velocity components generated due to the flow through the slot decay rapidly in the transverse direction. A vortex-like flow existed on the single slotted wall for natural ventilation but diminished with increased suction flow rate. For flow on a segmented multi-slotted wall, the normal velocity component changes were found to be maximum for measurement points located between the segmented slots atop the active chamber. The lateral influence due to applied suction and blowing, through a compartment, exceeded only slightly that in the downstream direction. Limited upstream influence was observed. Influence coefficients were determined from the data in the least-square sense for blowing and suction applied through one and two compartments. This was found to be an adequate determination of the influence coefficients for the range of mass flows considered.

Experimental Reacting Hydrogen Shear Layer Data at High Subsonic Mach Number

NASA Technical Reports Server (NTRS)

Chang, C. T.; Marek, C. J.; Wey, C.; Wey, C. C.

1996-01-01

The flow in a planar shear layer of hydrogen reacting with hot air was measured with a two-component laser Doppler velocimeter (LDV) system, a schlieren system, and OH fluorescence imaging. It was compared with a similar air-to-air case without combustion. The high-speed stream's flow speed was about 390 m/s, or Mach 0.71, and the flow speed ratio was 0.34. The results showed that a shear layer with reaction grows faster than one without; both cases are within the range of data scatter presented by the established data base. The coupling between the streamwise and the cross-stream turbulence components inside the shear layers was low, and reaction only increased it slightly. However, the shear layer shifted laterally into the lower speed fuel stream, and a more organized pattern of Reynolds stress was present in the reaction shear layer, likely as a result of the formation of a larger scale structure associated with shear layer corrugation from heat release. Dynamic pressure measurements suggest that coherent flow perturbations existed inside the shear layer and that this flow became more chaotic as the flow advected downstream. Velocity and thermal variable values are listed in this report for a computational fluid dynamics (CFD) benchmark.

In vivo lateral blood flow velocity measurement using speckle size estimation.

PubMed

Xu, Tiantian; Hozan, Mohsen; Bashford, Gregory R

2014-05-01

In previous studies, we proposed blood measurement using speckle size estimation, which estimates the lateral component of blood flow within a single image frame based on the observation that the speckle pattern corresponding to blood reflectors (typically red blood cells) stretches (i.e., is "smeared") if blood flow is in the same direction as the electronically controlled transducer line selection in a 2-D image. In this observational study, the clinical viability of ultrasound blood flow velocity measurement using speckle size estimation was investigated and compared with that of conventional spectral Doppler of carotid artery blood flow data collected from human patients in vivo. Ten patients (six male, four female) were recruited. Right carotid artery blood flow data were collected in an interleaved fashion (alternating Doppler and B-mode A-lines) with an Antares Ultrasound Imaging System and transferred to a PC via the Axius Ultrasound Research Interface. The scanning velocity was 77 cm/s, and a 4-s interval of flow data were collected from each subject to cover three to five complete cardiac cycles. Conventional spectral Doppler data were collected simultaneously to compare with estimates made by speckle size estimation. The results indicate that the peak systolic velocities measured with the two methods are comparable (within ±10%) if the scan velocity is greater than or equal to the flow velocity. When scan velocity is slower than peak systolic velocity, the speckle stretch method asymptotes to the scan velocity. Thus, the speckle stretch method is able to accurately measure pure lateral flow, which conventional Doppler cannot do. In addition, an initial comparison of the speckle size estimation and color Doppler methods with respect to computational complexity and data acquisition time indicated potential time savings in blood flow velocity estimation using speckle size estimation. Further studies are needed for calculation of the speckle stretch method across a field of view and combination with an appropriate axial flow estimator. Copyright © 2014 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.

Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cary, Robert E.

2015-12-08

Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.

Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cary, Robert B.

Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.

Evaluating post-wildfire hydrologic recovery using ParFlow in southern California

NASA Astrophysics Data System (ADS)

Lopez, S. R.; Kinoshita, A. M.; Atchley, A. L.

2016-12-01

Wildfires are naturally occurring hazards that can have catastrophic impacts. They can alter the natural processes within a watershed, such as surface runoff and subsurface water storage. Generally, post-fire hydrologic models are either one-dimensional, empirically-based models, or two-dimensional, conceptually-based models with lumped parameter distributions. These models are useful in providing runoff measurements at the watershed outlet; however, do not provide distributed hydrologic simulation at each point within the watershed. This research demonstrates how ParFlow, a three-dimensional, distributed hydrologic model can simulate post-fire hydrologic processes by representing soil burn severity (via hydrophobicity) and vegetation recovery as they vary both spatially and temporally. Using this approach, we are able to evaluate the change in post-fire water components (surface flow, lateral flow, baseflow, and evapotranspiration). This model is initially developed for a hillslope in Devil Canyon, burned in 2003 by the Old Fire in southern California (USA). The domain uses a 2m-cell size resolution over a 25 m by 25 m lateral extent. The subsurface reaches 2 m and is assigned a variable cell thickness, allowing an explicit consideration of the soil burn severity throughout the stages of recovery and vegetation regrowth. Vegetation regrowth is incorporated represented by satellite-based Enhanced Vegetation Index (EVI) products. The pre- and post-fire surface runoff, subsurface storage, and surface storage interactions are evaluated and will be used as a basis for developing a watershed-scale model. Long-term continuous simulations will advance our understanding of post-fire hydrological partitioning between water balance components and the spatial variability of watershed processes, providing improved guidance for post-fire watershed management.

MDR/Omni-band Reconfigurable Terminal: Design Concept

DTIC Science & Technology

1998-09-01

tasks, data bases and major communications flows. Global issues relevant to most of the blocks are then covered. Finally the planned sequence of...and event logger that are detailed in later paragraphs. The BITE(built-in test equipment)/Debugger detail can be found separately in the Global Issues paragraphs...conditions. Every part of the simulator has a BITE/Debugger component, the general description of which is given in Global Issues . Simulator control

Shared brain lateralization patterns in language and Acheulean stone tool production: a functional transcranial Doppler ultrasound study.

PubMed

Uomini, Natalie Thaïs; Meyer, Georg Friedrich

2013-01-01

The popular theory that complex tool-making and language co-evolved in the human lineage rests on the hypothesis that both skills share underlying brain processes and systems. However, language and stone tool-making have so far only been studied separately using a range of neuroimaging techniques and diverse paradigms. We present the first-ever study of brain activation that directly compares active Acheulean tool-making and language. Using functional transcranial Doppler ultrasonography (fTCD), we measured brain blood flow lateralization patterns (hemodynamics) in subjects who performed two tasks designed to isolate the planning component of Acheulean stone tool-making and cued word generation as a language task. We show highly correlated hemodynamics in the initial 10 seconds of task execution. Stone tool-making and cued word generation cause common cerebral blood flow lateralization signatures in our participants. This is consistent with a shared neural substrate for prehistoric stone tool-making and language, and is compatible with language evolution theories that posit a co-evolution of language and manual praxis. In turn, our results support the hypothesis that aspects of language might have emerged as early as 1.75 million years ago, with the start of Acheulean technology.

Species Specific Bacterial Spore Detection Using Lateral-Flow Immunoassay with DPA-Triggered Tb Luminescence

NASA Technical Reports Server (NTRS)

Ponce, Adrian

2003-01-01

A method of detecting bacterial spores incorporates (1) A method of lateral-flow immunoassay in combination with (2) A method based on the luminescence of Tb3+ ions to which molecules of dipicolinic acid (DPA) released from the spores have become bound. The present combination of lateral-flow immunoassay and DPA-triggered Tb luminescence was developed as a superior alternative to a prior lateral-flow immunoassay method in which detection involves the visual observation and/or measurement of red light scattered from colloidal gold nanoparticles. The advantage of the present combination method is that it affords both (1) High selectivity for spores of the species of bacteria that one seeks to detect (a characteristic of lateral-flow immunoassay in general) and (2) Detection sensitivity much greater (by virtue of the use of DPA-triggered Tb luminescence instead of gold nanoparticles) than that of the prior lateral-flow immunoassay method

Measurement and evaluation of swirl-type flow in labyrinth seals of conventional turbine stages

NASA Technical Reports Server (NTRS)

Hauck, L.

1982-01-01

The effects of load factor and rotor eccentricity were determined on flow conditions in test series for two stages. The results indicate that swirl-type entry flow follows the rules of potential swirl. Within the labyrinth cavities two spatial separated flow areas are considered. A dominating flow in periphera direction nearly fills the space between the sealing strips and the ceiling of the cavity. Below this flow, an area of axial mass transport is situated, with a slight peripheral component, limited on the nearest surroundings of the seals gap and the rotor surface. Between both flows, an exchange of energy takes place. Within the gaps, flow direction depends on axial velocity and therefore on variable flow contraction. A balance of energy within the seal and the cavities interprets the results of lateral force measurements as an influence of friction at the sealing strips surface and the rotating shaft surface. Stages with their blades put together in buckets by means of shrouding segments are particularly influenced by the rotating speed of the shrouding.

Deduction of two-dimensional blood flow vector by dual angle diverging waves from a cardiac sector probe

NASA Astrophysics Data System (ADS)

Maeda, Moe; Nagaoka, Ryo; Ikeda, Hayato; Yaegashi, So; Saijo, Yoshifumi

2018-07-01

Color Doppler method is widely used for noninvasive diagnosis of heart diseases. However, the method can measure one-dimensional (1D) blood flow velocity only along an ultrasonic beam. In this study, diverging waves with two different angles were irradiated from a cardiac sector probe to estimate a two-dimensional (2D) blood flow vector from each velocity measured with the angles. The feasibility of the proposed method was evaluated in experiments using flow poly(vinyl alcohol) (PVA) gel phantoms. The 2D velocity vectors obtained with the proposed method were compared with the flow vectors obtained with the particle image velocimetry (PIV) method. Root mean square errors of the axial and lateral components were 11.3 and 29.5 mm/s, respectively. The proposed method was also applied to echo data from the left ventricle of the heart. The inflow from the mitral valve in diastole and the ejection flow concentrating in the aorta in systole were visualized.

Analysis of the NASA/MSFC airborne Doppler lidar results from San Gorgonio Pass, California

NASA Technical Reports Server (NTRS)

Cliff, W. C.; Skarda, J. R.; Renne, D. S.; Sandusky, W. F.

1985-01-01

The NASA/MSFC Airborne Doppler Lidar System was flown in July 1981 aboard the NASA/Ames Convair 990 on the east side of San Gorgonio Pass California, near Palm Springs, to measure and investigate the accelerated atmospheric wind field discharging from the pass. At this region, the maritime layer from the west coast accelerates through the pass and spreads out over the valley floor on the east side of the pass. The experiment was selected in order to study accelerated flow in and at the exit of the canyon. Ground truth wind data taken concurrently with the flight data were available from approximately 12 meteorological towers and 3 tala kites for limited comparison purposes. The experiment provided the first spatial data for ensemble averaging of spatial correlations to compute lateral and longitudinal length scales in the lateral and longitudinal directions for both components, and information on atmospheric flow in this region of interest from wind energy resource considerations.

Low speed wind tunnel test of a propulsive wing/canard concept in the STOL configuration. Volume 2: Test data

NASA Technical Reports Server (NTRS)

Stewart, V. R.

1987-01-01

A propulsive wind/canard model was tested at STOL operating conditions in the NASA Langley Research Center 4 x 7 meter wind tunnel. Longitudinal and lateral/directional aerodynamic characteristics were measured for various flap deflections, angles of attack and sideslip, and blowing coefficients. Testing was conducted for several model heights to determine ground proximity effects on the aerodynamic characteristics. Flow field surveys of local flow angles and velocities were performed behind both the canard and the wing. This is volume 2 of a 2 volume report. All of the test data in three appendices are presented. Appendix A presented tabulated six component force and moment data, Appendix B presents tabulated wing pressure coefficients, and Appendix C presents the flow field data.

Development of a hybrid 3-D hydrological model to simulate hillslopes and the regional unconfined aquifer system in Earth system models

NASA Astrophysics Data System (ADS)

Hazenberg, P.; Broxton, P. D.; Brunke, M.; Gochis, D.; Niu, G. Y.; Pelletier, J. D.; Troch, P. A. A.; Zeng, X.

2015-12-01

The terrestrial hydrological system, including surface and subsurface water, is an essential component of the Earth's climate system. Over the past few decades, land surface modelers have built one-dimensional (1D) models resolving the vertical flow of water through the soil column for use in Earth system models (ESMs). These models generally have a relatively coarse model grid size (~25-100 km) and only account for sub-grid lateral hydrological variations using simple parameterization schemes. At the same time, hydrologists have developed detailed high-resolution (~0.1-10 km grid size) three dimensional (3D) models and showed the importance of accounting for the vertical and lateral redistribution of surface and subsurface water on soil moisture, the surface energy balance and ecosystem dynamics on these smaller scales. However, computational constraints have limited the implementation of the high-resolution models for continental and global scale applications. The current work presents a hybrid-3D hydrological approach is presented, where the 1D vertical soil column model (available in many ESMs) is coupled with a high-resolution lateral flow model (h2D) to simulate subsurface flow and overland flow. H2D accounts for both local-scale hillslope and regional-scale unconfined aquifer responses (i.e. riparian zone and wetlands). This approach was shown to give comparable results as those obtained by an explicit 3D Richards model for the subsurface, but improves runtime efficiency considerably. The h3D approach is implemented for the Delaware river basin, where Noah-MP land surface model (LSM) is used to calculated vertical energy and water exchanges with the atmosphere using a 10km grid resolution. Noah-MP was coupled within the WRF-Hydro infrastructure with the lateral 1km grid resolution h2D model, for which the average depth-to-bedrock, hillslope width function and soil parameters were estimated from digital datasets. The ability of this h3D approach to simulate the hydrological dynamics of the Delaware River basin will be assessed by comparing the model results (both hydrological performance and numerical efficiency) with the standard setup of the NOAH-MP model and a high-resolution (1km) version of NOAH-MP, which also explicitly accounts for lateral subsurface and overland flow.

Spatial orientation of caloric nystagmus in semicircular canal-plugged monkeys.

PubMed

Arai, Yasuko; Yakushin, Sergei B; Cohen, Bernard; Suzuki, Jun-Ichi; Raphan, Theodore

2002-08-01

We studied caloric nystagmus before and after plugging all six semicircular canals to determine whether velocity storage contributed to the spatial orientation of caloric nystagmus. Monkeys were stimulated unilaterally with cold ( approximately 20 degrees C) water while upright, supine, prone, right-side down, and left-side down. The decline in the slow phase velocity vector was determined over the last 37% of the nystagmus, at a time when the response was largely due to activation of velocity storage. Before plugging, yaw components varied with the convective flow of endolymph in the lateral canals in all head orientations. Plugging blocked endolymph flow, eliminating convection currents. Despite this, caloric nystagmus was readily elicited, but the horizontal component was always toward the stimulated (ipsilateral) side, regardless of head position relative to gravity. When upright, the slow phase velocity vector was close to the yaw and spatial vertical axes. Roll components became stronger in supine and prone positions, and vertical components were enhanced in side down positions. In each case, this brought the velocity vectors toward alignment with the spatial vertical. Consistent with principles governing the orientation of velocity storage, when the yaw component of the velocity vector was positive, the cross-coupled pitch or roll components brought the vector upward in space. Conversely, when yaw eye velocity vector was downward in the head coordinate frame, i.e., negative, pitch and roll were downward in space. The data could not be modeled simply by a reduction in activity in the ipsilateral vestibular nerve, which would direct the velocity vector along the roll direction. Since there is no cross coupling from roll to yaw, velocity storage alone could not rotate the vector to fit the data. We postulated, therefore, that cooling had caused contraction of the endolymph in the plugged canals. This contraction would deflect the cupula toward the plug, simulating ampullofugal flow of endolymph. Inhibition and excitation induced by such cupula deflection fit the data well in the upright position but not in lateral or prone/supine conditions. Data fits in these positions required the addition of a spatially orientated, velocity storage component. We conclude, therefore, that three factors produce cold caloric nystagmus after canal plugging: inhibition of activity in ampullary nerves, contraction of endolymph in the stimulated canals, and orientation of eye velocity to gravity through velocity storage. Although the response to convection currents dominates the normal response to caloric stimulation, velocity storage probably also contributes to the orientation of eye velocity.

Investigation of the flow structure in thin polymer films using 3D µPTV enhanced by GPU

NASA Astrophysics Data System (ADS)

Cavadini, Philipp; Weinhold, Hannes; Tönsmann, Max; Chilingaryan, Suren; Kopmann, Andreas; Lewkowicz, Alexander; Miao, Chuan; Scharfer, Philip; Schabel, Wilhelm

2018-04-01

To understand the effects of inhomogeneous drying on the quality of polymer coatings, an experimental setup to resolve the occurring flow field throughout the drying film has been developed. Deconvolution microscopy is used to analyze the flow field in 3D and time. Since the dimension of the spatial component in the direction of the line-of-sight is limited compared to the lateral components, a multi-focal approach is used. Here, the beam of light is equally distributed on up to five cameras using cubic beam splitters. Adding a meniscus lens between each pair of camera and beam splitter and setting different distances between each camera and its meniscus lens creates multi-focality and allows one to increase the depth of the observed volume. Resolving the spatial component in the line-of-sight direction is based on analyzing the point spread function. The analysis of the PSF is computational expensive and introduces a high complexity compared to traditional particle image velocimetry approaches. A new algorithm tailored to the parallel computing architecture of recent graphics processing units has been developed. The algorithm is able to process typical images in less than a second and has further potential to realize online analysis in the future. As a prove of principle, the flow fields occurring in thin polymer solutions drying at ambient conditions and at boundary conditions that force inhomogeneous drying are presented.

Variable parameter McCarthy-Muskingum routing method considering lateral flow

NASA Astrophysics Data System (ADS)

Yadav, Basant; Perumal, Muthiah; Bardossy, Andras

2015-04-01

The fully mass conservative variable parameter McCarthy-Muskingum (VPMM) method recently proposed by Perumal and Price (2013) for routing floods in channels and rivers without considering lateral flow is extended herein for accounting uniformly distributed lateral flow contribution along the reach. The proposed procedure is applied for studying flood wave movement in a 24.2 km river stretch between Rottweil and Oberndorf gauging stations of Neckar River in Germany wherein significant lateral flow contribution by intermediate catchment rainfall prevails during flood wave movement. The geometrical elements of the cross-sectional information of the considered routing river stretch without considering lateral flow are estimated using the Robust Parameter Estimation (ROPE) algorithm that allows for arriving at the best performing set of bed width and side slope of a trapezoidal section. The performance of the VPMM method is evaluated using the Nash-Sutcliffe model efficiency criterion as the objective function to be maximized using the ROPE algorithm. The twenty-seven flood events in the calibration set are considered to identify the relationship between 'total rainfall' and 'total losses' as well as to optimize the geometric characteristics of the prismatic channel (width and slope of the trapezoidal section). Based on this analysis, a relationship between total rainfall and total loss of the intermediate catchment is obtained and then used to estimate the lateral flow in the reach. Assuming the lateral flow hydrograph is of the form of inflow hydrograph and using the total intervening catchment runoff estimated from the relationship, the uniformly distributed lateral flow rate qL at any instant of time is estimated for its use in the VPMM routing method. All the 27 flood events are simulated using this routing approach considering lateral flow along the reach. Many of these simulations are able to simulate the observed hydrographs very closely. The proposed approach of accounting lateral flow using the VPMM method is independently verified by routing flood hydrograph of 6 flood events which are not used in the total rainfall vs total loss relationship established for the intervening catchment of the studied river reach. Close reproduction of the outflow hydrographs of these independent events using the proposed VPMM method accounting for lateral flow demonstrate the practical utility of the method.

Studies with sample conductivity, insertion rates, and particle deflection in a continuous flow electrophoresis system

NASA Technical Reports Server (NTRS)

Williams, G., Jr.

1982-01-01

The continuous flow electrophoresis system makes electrophoresis possible in a free-flowing film of aqueous electrolyte medium. The sample continuously enters the electrolyte at the top of the chamber and is subjected to the action of a lateral dc field. This divides the sample into fractions since each component has a distinctive electrophoretic mobility. Tests were made using monodisperse polystyrene latex microspheres to determine optimum sample conductivity, insertion rates and optimum electric field applications as baseline data for future STS flight experiments. Optimum sample flow rates for the selected samples were determined to be approximately 26 micro-liters/min. Experiments with samples in deionized water yielded best results and voltages in the 20 V/cm to 30 V/cm range were optimum. Deflections of formaldehyde fixed turkey and bovine erythrocytes were determined using the continuous flow electrophoresis system. The effects of particle interactions on sample resolution and migration in the chamber was also evaluated.

Shallow subsurface temperatures and some estimates of heat flow from the Colorado Plateau of northeastern Arizona

USGS Publications Warehouse

Sass, J.H.; Stone, Claudia; Bills, D.J.

1982-01-01

Temperature data to depths of a few hundred meters were obtained from 29 wells in northeastern Arizona; 12 in the region surrounding the San Francisco Volcanic Field, 8 in the Black Mesa area, and 9 in the south-central Colorado Plateau which includes the White Mountains. Although there was evidence for local hydrologic disturbances in many temperature profiles, most wells provided an estimate of the conductive thermal gradient at the site. A few thermal conductivities were measured and were combined with published regional averages for the north-central part of the Colorado Plateau to produce crude estimates of regional heat flux. None of the wells was accessible below the regional aquifers. To these depths, heat flow in the area of the San Francisco Volcanic Field appears to be controlled primarily by regional lateral water movement having a significant downward vertical component of velocity. The mean heat flow of 27?5 mW^-2 is only a third to a quarter of what we would expect in this tectonic setting. The heat that is being carried laterally and downward probably is being discharged at low enthalpy and low elevation in springs and streams of the Colorado Plateau and Mogollon Rim. In the vicinity of Black Mesa, heat-flow averages about 60 mW^-2, characteristic of the 'cool interior' of the Colorado Plateau. North of the White Mountain Volcanic Field, the average heat flow is about 95 mW^-2.

Heat Transfer to Anode of Arc as Function of Transverse Magnetic Field and Lateral Gas Flow Velocity

NASA Astrophysics Data System (ADS)

Zama, Yoshiyuki; Shiino, Toru; Ishii, Yoko; Maeda, Yoshifumi; Yamamoto, Shinji; Iwao, Toru

2016-10-01

Gas tungsten arc welding has useful joining technology because of high-energy and high-current characteristics. It can be flexible from the transverse magnetic field and lateral gas flow velocity. In this case, the weld defect occurs. In this research, the heat transfer to the anode of the arc as a function of the transverse magnetic field and lateral gas flow velocity is elucidated. That magnetic flux density and lateral gas velocity were varied from 0 to 3 mT and 0 to 50?m?s -1, respectively. The axial plasma gas argon flow rates were 3?slm. A transverse magnetic field is applied to the arc using Helmholtz coil. The anode is used by a water-cooled copper plate, and the heat transfer is measured by temperature of cooled water. As a result, the arc is deflected by the Lorentz force and lateral gas convection. Thus, the heat transfer to the anode of the arc decreases with increasing the transverse magnetic field and lateral gas flow velocity. In addition, the heat transfer to the anode changes with different attachments modes. The lateral gas flow causes a convective heat loss from the arc to the chamber walls.

[Effect of hypoxia on glycolysis in the adductor muscle and hepatopancreas of the marine mussel Mytilus galloprovincialis Lmk].

PubMed

Ibarguren, I; Villamarín, J A; Barcia, R; Ramos-Martínez, J I

1989-12-01

Concentrations of glycolytic intermediates and adenine nucleotides have been estimated in adductor muscle and hepatopancreas from the sea mussel Mytilus galloprovincialis Lmk. after various periods of valve closure. Mass action ratios of enzyme steps involved in the metabolism of these components are compared with their equilibrium constants. This reveals hexokinase, phosphofructokinase, pyruvate kinase and fructose-1,6-bisphosphatase catalyze non-equilibrium reactions. The changes in the concentrations of the glycolytic intermediates and in the rate M.A.R./Keq during hypoxia suggest that the carbon flow after valve closure is first controlled by phophofructokinase, but later on the rate of transformation of phosphoenolyruvate regulates this flow.

Origin of arsenic in groundwater from the multilayer aquifer in Cremona (northern Italy).

PubMed

Rotiroti, Marco; Sacchi, Elisa; Fumagalli, Letizia; Bonomi, Tullia

2014-05-20

An analysis of 70 wells that tap groundwater from depths of up to 260 m in and around the town of Cremona, N. Italy, shows that 50 of them contain more than 10 μg/L of arsenic. Concentrations of As >10 ppb are accompanied by concentrations of Fe ranging from <0.1 to 6 mg/L and high concentrations of NH4 and Mn (<19 and <1.3 mg/L, respectively). The associations suggest that the mechanism of mobilization of As is the reductive dissolution of Fe oxides driven by the degradation of peat, which is commonly found in the aquifer system. Groundwater in the aquifer has a component of downward flow via leakage through aquitards and flow through lateral discontinuities in them. Along these flow paths, As is released by reductive dissolution of Fe oxides in shallow and intermediate aquifers (0-85 m below surface), reaching up to 183 μg/L, and is attenuated (<95 μg/L) at greater depths (100-150 m). Coprecipitation in iron sulfides could play an important role in As attenuation at these depths. The lower As concentration (<37 μg/L) in the deepest aquifer (160-260 m) is less related to the As concentration of the overlying aquifers because the groundwater here has a component of upward flow.

Use of sinkhole and specific capacity distributions to assess vertical gradients in a karst aquifer

USGS Publications Warehouse

McCoy, K.J.; Kozar, M.D.

2008-01-01

The carbonate-rock aquifer in the Great Valley, West Virginia, USA, was evaluated using a database of 687 sinkholes and 350 specific capacity tests to assess structural, lithologic, and topographic influences on the groundwater flow system. The enhanced permeability of the aquifer is characterized in part by the many sinkholes, springs, and solutionally enlarged fractures throughout the valley. Yet, vertical components of subsurface flow in this highly heterogeneous aquifer are currently not well understood. To address this problem, this study examines the apparent relation between geologic features of the aquifer and two spatial indices of enhanced permeability attributed to aquifer karstification: (1) the distribution of sinkholes and (2) the occurrence of wells with relatively high specific capacity. Statistical results indicate that sinkholes (funnel and collapse) occur primarily along cleavage and bedding planes parallel to subparallel to strike where lateral or downward vertical gradients are highest. Conversely, high specific capacity values are common along prominent joints perpendicular or oblique to strike. The similarity of the latter distribution to that of springs suggests these fractures are areas of upward-convergent flow. These differences between sinkhole and high specific capacity distributions suggest vertical flow components are primarily controlled by the orientation of geologic structure and associated subsurface fracturing. ?? 2007 Springer-Verlag.

Hypogenic origin, geologic controls and functional organization of a giant cave system in Precambrian carbonates, Brazil

NASA Astrophysics Data System (ADS)

Klimchouk, Alexander; Auler, Augusto S.; Bezerra, Francisco H. R.; Cazarin, Caroline L.; Balsamo, Fabrizio; Dublyansky, Yuri

2016-01-01

This study is focused on speleogenesis of the Toca da Boa Vista (TBV) and Toca da Barriguda (TBR), the longest caves in South America occurring in the Neoproterozoic Salitre Formation in the São Francisco Craton, NE Brazil. We employ a multidisciplinary approach integrating detailed speleomorphogenetic, lithostratigraphic and geological structure studies in order to reveal the origin of the caves, their functional organization and geologic controls on their development. The caves developed in deep-seated confined conditions by rising flow. The overall fields of passages of TBV and TBR caves represent a speleogenetically exploited large NE-SW-trending fracture corridor associated with a major thrust. This corridor vertically extends across the Salitre Formation allowing the rise of deep fluids. In the overall ascending flow system, the formation of the cave pattern was controlled by a system of sub-parallel anticlines and troughs with NNE-SSW dominant orientation, and by vertical and lateral heterogeneities in fracture distribution. Three cave-stratigraphic stories reflect the actual hydrostratigraphy during the main phase of speleogenesis. Cavities at different stories are distinct in morphology and functioning. The gross tree-dimensional pattern of the system is effectively organized to conduct rising flow in deep-seated confined conditions. Cavities in the lower story developed as recharge components to the system. A laterally extensive conduit network in the middle story formed because the vertical flow from numerous recharge points has been redirected laterally along the highly conductive unit, occurring below the major seal - a scarcely fractured unit. Rift-like and shaft-like conduits in the upper story developed along fracture-controlled outflow paths, breaching the integrity of the major seal, and served as outlets for the cave system. The cave system represents a series of vertically organized, functionally largely independent clusters of cavities developed within individual ascending flow cells. Lateral integration of clusters occurred due to hydrodynamic interaction between the flow cells in course of speleogenetic evolution and change of boundary conditions. The main speleogenetic phase, during which the gross cave pattern has been established and the caves acquired most of their volume, was likely related to rise of deep fluids at about 520 Ma or associated with rifting and the Pangea break-up in Triassic-Cretaceous. This study highlights the importance of speleogenetic studies for interpreting porosity and permeability features in carbonate reservoirs.

Direction of unsaturated flow in a homogeneous and isotropic hillslope

USGS Publications Warehouse

Lu, Ning; Kaya, Basak Sener; Godt, Jonathan W.

2011-01-01

The distribution of soil moisture in a homogeneous and isotropic hillslope is a transient, variably saturated physical process controlled by rainfall characteristics, hillslope geometry, and the hydrological properties of the hillslope materials. The major driving mechanisms for moisture movement are gravity and gradients in matric potential. The latter is solely controlled by gradients of moisture content. In a homogeneous and isotropic saturated hillslope, absent a gradient in moisture content and under the driving force of gravity with a constant pressure boundary at the slope surface, flow is always in the lateral downslope direction, under either transient or steady state conditions. However, under variably saturated conditions, both gravity and moisture content gradients drive fluid motion, leading to complex flow patterns. In general, the flow field near the ground surface is variably saturated and transient, and the direction of flow could be laterally downslope, laterally upslope, or vertically downward. Previous work has suggested that prevailing rainfall conditions are sufficient to completely control these flow regimes. This work, however, shows that under time-varying rainfall conditions, vertical, downslope, and upslope lateral flow can concurrently occur at different depths and locations within the hillslope. More importantly, we show that the state of wetting or drying in a hillslope defines the temporal and spatial regimes of flow and when and where laterally downslope and/or laterally upslope flow occurs.

Direction of unsaturated flow in a homogeneous and isotropic hillslope

USGS Publications Warehouse

Lu, N.; Kaya, B.S.; Godt, J.W.

2011-01-01

The distribution of soil moisture in a homogeneous and isotropic hillslope is a transient, variably saturated physical process controlled by rainfall characteristics, hillslope geometry, and the hydrological properties of the hillslope materials. The major driving mechanisms for moisture movement are gravity and gradients in matric potential. The latter is solely controlled by gradients of moisture content. In a homogeneous and isotropic saturated hillslope, absent a gradient in moisture content and under the driving force of gravity with a constant pressure boundary at the slope surface, flow is always in the lateral downslope direction, under either transient or steady state conditions. However, under variably saturated conditions, both gravity and moisture content gradients drive fluid motion, leading to complex flow patterns. In general, the flow field near the ground surface is variably saturated and transient, and the direction of flow could be laterally downslope, laterally upslope, or vertically downward. Previous work has suggested that prevailing rainfall conditions are sufficient to completely control these flow regimes. This work, however, shows that under time-varying rainfall conditions, vertical, downslope, and upslope lateral flow can concurrently occur at different depths and locations within the hillslope. More importantly, we show that the state of wetting or drying in a hillslope defines the temporal and spatial regimes of flow and when and where laterally downslope and/or laterally upslope flow occurs. Copyright 2011 by the American Geophysical Union.

Spot test kit for explosives detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pagoria, Philip F; Whipple, Richard E; Nunes, Peter J

An explosion tester system comprising a body, a lateral flow membrane swab unit adapted to be removeably connected to the body, a first explosives detecting reagent, a first reagent holder and dispenser operatively connected to the body, the first reagent holder and dispenser containing the first explosives detecting reagent and positioned to deliver the first explosives detecting reagent to the lateral flow membrane swab unit when the lateral flow membrane swab unit is connected to the body, a second explosives detecting reagent, and a second reagent holder and dispenser operatively connected to the body, the second reagent holder and dispensermore » containing the second explosives detecting reagent and positioned to deliver the second explosives detecting reagent to the lateral flow membrane swab unit when the lateral flow membrane swab unit is connected to the body.« less

Research on Flow Field Perception Based on Artificial Lateral Line Sensor System.

PubMed

Liu, Guijie; Wang, Mengmeng; Wang, Anyi; Wang, Shirui; Yang, Tingting; Malekian, Reza; Li, Zhixiong

2018-03-11

In nature, the lateral line of fish is a peculiar and important organ for sensing the surrounding hydrodynamic environment, preying, escaping from predators and schooling. In this paper, by imitating the mechanism of fish lateral canal neuromasts, we developed an artificial lateral line system composed of micro-pressure sensors. Through hydrodynamic simulations, an optimized sensor structure was obtained and the pressure distribution models of the lateral surface were established in uniform flow and turbulent flow. Carrying out the corresponding underwater experiment, the validity of the numerical simulation method is verified by the comparison between the experimental data and the simulation results. In addition, a variety of effective research methods are proposed and validated for the flow velocity estimation and attitude perception in turbulent flow, respectively and the shape recognition of obstacles is realized by the neural network algorithm.

Colorimetric chemical analysis sampler for the presence of explosives

DOEpatents

Nunes, Peter J [Danville, CA; Del Eckels, Joel [Livermore, CA; Reynolds, John G [San Ramon, CA; Pagoria, Philip F [Livermore, CA; Simpson, Randall L [Livermore, CA

2011-09-27

A tester for testing for explosives comprising a body, a lateral flow swab unit operably connected to the body, a explosives detecting reagent contained in the body, and a dispenser operatively connected to the body and the lateral flow swab unit. The dispenser selectively allows the explosives detecting reagent to be delivered to the lateral flow swab unit.

Colorimetric chemical analysis sampler for the presence of explosives

DOEpatents

Nunes, Peter J.; Eckels, Joel Del; Reynolds, John G.; Pagoria, Philip F.; Simpson, Randall L.

2014-07-01

A tester for testing for explosives comprising a body, a lateral flow swab unit operably connected to the body, a explosives detecting reagent contained in the body, and a dispenser operatively connected to the body and the lateral flow swab unit. The dispenser selectively allows the explosives detecting reagent to be delivered to the lateral flow swab unit.

Correlation Between Contrast Time-Density Time on Digital Subtraction Angiography and Flow: An in Vitro Study.

PubMed

Brunozzi, Denise; Shakur, Sophia F; Ismail, Rahim; Linninger, Andreas; Hsu, Chih-Yang; Charbel, Fady T; Alaraj, Ali

2018-02-01

Digital subtraction angiography (DSA) provides an excellent anatomic characterization of cerebral vasculature, but hemodynamic assessment is often qualitative and subjective. Various clinical algorithms have been produced to semiquantify flow from the data obtained from DSA, but few have tested them against reliable flow values. An arched flow model was created and injected with contrast material. Seventeen injections were acquired in anterior-posterior and lateral DSA projections, and 4 injections were acquired in oblique projection. Image intensity change over the angiogram cycle of each DSA run was analyzed through a custom MATLAB code. Time-density plots obtained were divided into 3 components (time-density times, TDTs): TDT 10%-100% (time needed for contrast material to change image intensity from 10% to 100%), TDT 100%-10% (time needed for contrast material to change image intensity from 100% to 10%), and TDT 25%-25% (time needed for contrast material to change from 25% image intensity to 25%). Time-density index (TDI) was defined as model cross-sectional area to TDT ratio, and it was measured against different flow rates. TDI 10%-100% , TDI 100%-10% , and TDI 25%-25% all correlated significantly with flow (P < 0.001). TDI 10%-100% , TDI 100%-10% , and TDI 25%-25% showed, respectively, a correlation coefficient of 0.91, 0.91, and 0.97 in the anterior-posterior DSA projections (P < 0.001). In the lateral DSA projection, TDI 100%-10% showed a weaker correlation (r = 0.57; P = 0.03). Also in the oblique DSA projection, TDIs correlated significantly with flow. TDI on DSA correlates significantly with flow. Although in vitro studies might overlook conditions that occur in patients, this method appears to correlate with the flow and could offer a semiquantitative method to evaluate the cerebral blood flow. Copyright © 2017 Elsevier Inc. All rights reserved.

Modeling post-wildfire hydrological processes with ParFlow

NASA Astrophysics Data System (ADS)

Escobar, I. S.; Lopez, S. R.; Kinoshita, A. M.

2017-12-01

Wildfires alter the natural processes within a watershed, such as surface runoff, evapotranspiration rates, and subsurface water storage. Post-fire hydrologic models are typically one-dimensional, empirically-based models or two-dimensional, conceptually-based models with lumped parameter distributions. These models are useful for modeling and predictions at the watershed outlet; however, do not provide detailed, distributed hydrologic processes at the point scale within the watershed. This research uses ParFlow, a three-dimensional, distributed hydrologic model to simulate post-fire hydrologic processes by representing the spatial and temporal variability of soil burn severity (via hydrophobicity) and vegetation recovery. Using this approach, we are able to evaluate the change in post-fire water components (surface flow, lateral flow, baseflow, and evapotranspiration). This work builds upon previous field and remote sensing analysis conducted for the 2003 Old Fire Burn in Devil Canyon, located in southern California (USA). This model is initially developed for a hillslope defined by a 500 m by 1000 m lateral extent. The subsurface reaches 12.4 m and is assigned a variable cell thickness to explicitly consider soil burn severity throughout the stages of recovery and vegetation regrowth. We consider four slope and eight hydrophobic layer configurations. Evapotranspiration is used as a proxy for vegetation regrowth and is represented by the satellite-based Simplified Surface Energy Balance (SSEBOP) product. The pre- and post-fire surface runoff, subsurface storage, and surface storage interactions are evaluated at the point scale. Results will be used as a basis for developing and fine-tuning a watershed-scale model. Long-term simulations will advance our understanding of post-fire hydrological partitioning between water balance components and the spatial variability of watershed processes, providing improved guidance for post-fire watershed management. In reference to the presenter, Isabel Escobar: Research is funded by the NASA-DIRECT STEM Program. Travel expenses for this presentation is funded by CSU-LSAMP. CSU-LSAMP is supported by the National Science Foundation under Grant # HRD-1302873 and the CSU Office of Chancellor.

Research on Flow Field Perception Based on Artificial Lateral Line Sensor System

PubMed Central

Wang, Anyi; Wang, Shirui; Yang, Tingting

2018-01-01

In nature, the lateral line of fish is a peculiar and important organ for sensing the surrounding hydrodynamic environment, preying, escaping from predators and schooling. In this paper, by imitating the mechanism of fish lateral canal neuromasts, we developed an artificial lateral line system composed of micro-pressure sensors. Through hydrodynamic simulations, an optimized sensor structure was obtained and the pressure distribution models of the lateral surface were established in uniform flow and turbulent flow. Carrying out the corresponding underwater experiment, the validity of the numerical simulation method is verified by the comparison between the experimental data and the simulation results. In addition, a variety of effective research methods are proposed and validated for the flow velocity estimation and attitude perception in turbulent flow, respectively and the shape recognition of obstacles is realized by the neural network algorithm. PMID:29534499

A low-temperature ductile shear zone: The gypsum-dominated western extension of the brittle Fella-Sava Fault, Southern Alps.

PubMed

Bartel, Esther Maria; Neubauer, Franz; Heberer, Bianca; Genser, Johann

2014-12-01

Based on structural and fabric analyses at variable scales we investigate the evaporitic gypsum-dominated Comeglians-Paularo shear zone in the Southern Alps (Friuli). It represents the lateral western termination of the brittle Fella-Sava Fault. Missing dehydration products of gypsum and the lack of annealing indicate temperatures below 100 °C during development of the shear zone. Despite of such low temperatures the shear zone clearly exhibits mylonitic flow, thus evidencing laterally coeval activity of brittle and viscous deformation. The dominant structures within the gypsum rocks of the Lower Bellerophon Formation are a steeply to gently S-dipping foliation, a subhorizontal stretching lineation and pure shear-dominated porphyroclast systems. A subordinate simple shear component with dextral displacement is indicated by scattered σ-clasts. Both meso- and microscale structures are characteristic of a subsimple shear type of deformation with components of both coaxial and non-coaxial strain. Shortening in a transpressive regime was accommodated by right-lateral displacement and internal pure shear deformation within the Comeglians-Paularo shear zone. The shear zone shows evidence for a combination of two stretching faults, where stretching occurred in the rheologically weaker gypsum member and brittle behavior in enveloping lithologies.

Keratocytes Generate Traction Forces in Two PhasesV⃞

PubMed Central

Burton, Kevin; Park, Jung H.; Taylor, D. Lansing

1999-01-01

Forces generated by goldfish keratocytes and Swiss 3T3 fibroblasts have been measured with nanonewton precision and submicrometer spatial resolution. Differential interference contrast microscopy was used to visualize deformations produced by traction forces in elastic substrata, and interference reflection microscopy revealed sites of cell-substratum adhesions. Force ranged from a few nanonewtons at submicrometer spots under the lamellipodium to several hundred nanonewtons under the cell body. As cells moved forward, centripetal forces were applied by lamellipodia at sites that remained stationary on the substratum. Force increased and abruptly became lateral at the boundary of the lamellipodium and the cell body. When the cell retracted at its posterior margin, cell-substratum contact area decreased more rapidly than force, so that stress (force divided by area) increased as the cell pulled away. An increase in lateral force was associated with widening of the cell body. These mechanical data suggest an integrated, two-phase mechanism of cell motility: (1) low forces in the lamellipodium are applied in the direction of cortical flow and cause the cell body to be pulled forward; and (2) a component of force at the flanks pulls the rear margins forward toward the advancing cell body, whereas a large lateral component contributes to detachment of adhesions without greatly perturbing forward movement. PMID:10564269

Keratocytes generate traction forces in two phases.

PubMed

Burton, K; Park, J H; Taylor, D L

1999-11-01

Forces generated by goldfish keratocytes and Swiss 3T3 fibroblasts have been measured with nanonewton precision and submicrometer spatial resolution. Differential interference contrast microscopy was used to visualize deformations produced by traction forces in elastic substrata, and interference reflection microscopy revealed sites of cell-substratum adhesions. Force ranged from a few nanonewtons at submicrometer spots under the lamellipodium to several hundred nanonewtons under the cell body. As cells moved forward, centripetal forces were applied by lamellipodia at sites that remained stationary on the substratum. Force increased and abruptly became lateral at the boundary of the lamellipodium and the cell body. When the cell retracted at its posterior margin, cell-substratum contact area decreased more rapidly than force, so that stress (force divided by area) increased as the cell pulled away. An increase in lateral force was associated with widening of the cell body. These mechanical data suggest an integrated, two-phase mechanism of cell motility: (1) low forces in the lamellipodium are applied in the direction of cortical flow and cause the cell body to be pulled forward; and (2) a component of force at the flanks pulls the rear margins forward toward the advancing cell body, whereas a large lateral component contributes to detachment of adhesions without greatly perturbing forward movement.

Characterization of Turbulent Open Channel Flow in a Full-Scale Spiral Corrugated Culvert

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guensch, Greg R.; Richmond, Marshall C.; Tritico, Hans

2004-02-26

A micro-Acoustic Doppler Velocimeter (ADV) was used to characterize the three-dimensional velocity and turbulence characteristics in a full-scale culvert with spiral corrugations. The culvert was set up in a test bed constructed to examine juvenile salmon passage success in various culvert types. The test culvert was 12.2 m long and 1.83m in diameter, and set at a 1.14% slope. The corrugations were 2.54 cm deep by 7.62 cm peak to peak with a 5° right-handed pitch. Tailwater elevation was adjustable with a stop-log system and was set slightly above the water surface level at the culvert exit. Cross-sectional grids ofmore » ADV measurements were taken at discharges of 28, 57, 113, 227, and 453 lps at 9 locations within the culvert barrel and just inside the headwater and tailwater tanks. Results indicated that the spiral corrugations induced asymmetries in the velocity and turbulence distributions. These asymmetries caused a Reduced Velocity Zone (RVZ) on the right side of the culvert as seen looking upstream, which could aid small fish during upstream passage. Velocity and turbulence levels in the RVZ were found to be less than in mid channel or on the left side of the culvert, and the difference became greater at increased flow rates. Lateral and vertical velocity components were very small relative to the axial component, while lateral and vertical turbulence intensities were comparable to the axial component. Inlet loss coefficients were calculated as well and ranged from 0.32 to 0.42. Relationships between the average velocity and the velocity and turbulence intensity in the RVZ were developed, which may be useful for evaluating whether the barrel of a culvert is passable for juvenile fish.« less

Wave propagation and power flow in an acoustic metamaterial plate with lateral local resonance attachment

NASA Astrophysics Data System (ADS)

Wang, Ting; Sheng, Meiping; Ding, Xiaodong; Yan, Xiaowei

2018-03-01

This paper presents analysis on wave propagation and power flow in an acoustic metamaterial plate with lateral local resonance. The metamaterial is designed to have lateral local resonance systems attached to a homogeneous plate. Relevant theoretical analysis, numerical modelling and application prospect are presented. Results show that the metamaterial has two complete band gaps for flexural wave absorption and vibration attenuation. Damping can smooth and lower the metamaterial’s frequency responses in high frequency ranges at the expense of the band gap effect, and as an important factor to calculate the power flow is thoroughly investigated. Moreover, the effective mass density becomes negative and unbounded at specific frequencies. Simultaneously, power flow within band gaps are dramatically blocked from the power flow contour and power flow maps. Results from finite element modelling and power flow analysis reveal the working mechanism of the flexural wave attenuation and power flow blocked within the band gaps, where part of the flexural vibration is absorbed by the vertical resonator and the rest is transformed through four-link-mechanisms to the lateral resonators that oscillate and generate inertial forces indirectly to counterbalance the shear forces induced by the vibrational plate. The power flow is stored in the vertical and lateral local resonance, as well as in the connected plate.

Investigation of particle lateral migration in sample-sheath flow of viscoelastic fluid and Newtonian fluid.

PubMed

Yuan, Dan; Zhang, Jun; Yan, Sheng; Peng, Gangrou; Zhao, Qianbin; Alici, Gursel; Du, Hejun; Li, Weihua

2016-08-01

In this work, particle lateral migration in sample-sheath flow of viscoelastic fluid and Newtonian fluid was experimentally investigated. The 4.8-μm micro-particles were dispersed in a polyethylene oxide (PEO) viscoelastic solution, and then the solution was injected into a straight rectangular channel with a deionised (DI) water Newtonian sheath flow. Micro-particles suspended in PEO solution migrated laterally to a DI water stream, but migration in the opposite direction from a DI water stream to a PEO solution stream or from one DI water stream to another DI water stream could not be achieved. The lateral migration of particles depends on the viscoelastic properties of the sample fluids. Furthermore, the effects of channel length, flow rate, and PEO concentration were studied. By using viscoelastic sample flow and Newtonian sheath flow, a selective particle lateral migration can be achieved in a simple straight channel, without any external force fields. This particle lateral migration technique could be potentially used in solution exchange fields such as automated cell staining and washing in microfluidic platforms, and holds numerous biomedical applications. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Predictive techniques for river channel evolution and maintenance

USGS Publications Warehouse

Nelson, J.M.

1996-01-01

Predicting changes in alluvial channel morphology associated with anthropogenic and natural changes in flow and/or sediment supply is a critical part of the management of riverine systems. Over the past few years, advances in the understanding of the physics of sediment transport in conjunction with rapidly increasing capabilities in computational fluid dynamics have yielded now approaches to problems in river mechanics. Techniques appropriate for length scales ranging from reaches to bars and bedforms are described here. Examples of the use of these computational approaches are discussed for three cases: (1) the design of diversion scenarios that maintain channel morphology in steep cobble-bedded channels in Colorado, (2) determination of channel maintenance flows for the preservation of channel islands in the Snake River in Idaho, and (3) prediction of the temporal evolution of deposits in lateral separation zones for future assessment of the impacts of various dam release scenarios on lateral separation deposits in the Colorado River in Grand Canyon. With continued development of their scientific and technical components, the methodologies described here can provide powerful tools for the management of river environments in the future.

Geological factors affecting CO2 plume distribution

USGS Publications Warehouse

Frailey, S.M.; Leetaru, H.

2009-01-01

Understanding the lateral extent of a CO2 plume has important implications with regards to buying/leasing pore volume rights, defining the area of review for an injection permit, determining the extent of an MMV plan, and managing basin-scale sequestration from multiple injection sites. The vertical and lateral distribution of CO2 has implications with regards to estimating CO2 storage volume at a specific site and the pore pressure below the caprock. Geologic and flow characteristics such as effective permeability and porosity, capillary pressure, lateral and vertical permeability anisotropy, geologic structure, and thickness all influence and affect the plume distribution to varying degrees. Depending on the variations in these parameters one may dominate the shape and size of the plume. Additionally, these parameters do not necessarily act independently. A comparison of viscous and gravity forces will determine the degree of vertical and lateral flow. However, this is dependent on formation thickness. For example in a thick zone with injection near the base, the CO2 moves radially from the well but will slow at greater radii and vertical movement will dominate. Generally the CO2 plume will not appreciably move laterally until the caprock or a relatively low permeability interval is contacted by the CO2. Conversely, in a relatively thin zone with the injection interval over nearly the entire zone, near the wellbore the CO2 will be distributed over the entire vertical component and will move laterally much further with minimal vertical movement. Assuming no geologic structure, injecting into a thin zone or into a thick zone immediately under a caprock will result in a larger plume size. With a geologic structure such as an anticline, CO2 plume size may be restricted and injection immediately below the caprock may have less lateral plume growth because the structure will induce downward vertical movement of the CO2 until the outer edge of the plume reaches a spill point within the structure. ?? 2009 Elsevier Ltd. All rights reserved.

Inhibition of recombinase polymerase amplification by background DNA: a lateral flow-based method for enriching target DNA.

PubMed

Rohrman, Brittany; Richards-Kortum, Rebecca

2015-02-03

Recombinase polymerase amplification (RPA) may be used to detect a variety of pathogens, often after minimal sample preparation. However, previous work has shown that whole blood inhibits RPA. In this paper, we show that the concentrations of background DNA found in whole blood prevent the amplification of target DNA by RPA. First, using an HIV-1 RPA assay with known concentrations of nonspecific background DNA, we show that RPA tolerates more background DNA when higher HIV-1 target concentrations are present. Then, using three additional assays, we demonstrate that the maximum amount of background DNA that may be tolerated in RPA reactions depends on the DNA sequences used in the assay. We also show that changing the RPA reaction conditions, such as incubation time and primer concentration, has little effect on the ability of RPA to function when high concentrations of background DNA are present. Finally, we develop and characterize a lateral flow-based method for enriching the target DNA concentration relative to the background DNA concentration. This sample processing method enables RPA of 10(4) copies of HIV-1 DNA in a background of 0-14 μg of background DNA. Without lateral flow sample enrichment, the maximum amount of background DNA tolerated is 2 μg when 10(6) copies of HIV-1 DNA are present. This method requires no heating or other external equipment, may be integrated with upstream DNA extraction and purification processes, is compatible with the components of lysed blood, and has the potential to detect HIV-1 DNA in infant whole blood with high proviral loads.

Modeling the hydrological impacts of land use/land cover changes in the Andassa watershed, Blue Nile Basin, Ethiopia.

PubMed

Gashaw, Temesgen; Tulu, Taffa; Argaw, Mekuria; Worqlul, Abeyou W

2018-04-01

Understanding the hydrological response of a watershed to land use/land cover (LULC) changes is imperative for water resources management planning. The objective of this study was to analyze the hydrological impacts of LULC changes in the Andassa watershed for a period of 1985-2015 and to predict the LULC change impact on the hydrological status in year 2045. The hybrid land use classification technique for classifying Landsat images (1985, 2000 and 2015); Cellular-Automata Markov (CA-Markov) for prediction of the 2030 and 2045 LULC states; the Soil and Water Assessment Tool (SWAT) for hydrological modeling were employed in the analyses. In order to isolate the impacts of LULC changes, the LULC maps were used independently while keeping the other SWAT inputs constant. The contribution of each of the LULC classes was examined with the Partial Least Squares Regression (PLSR) model. The results showed that there was a continuous expansion of cultivated land and built-up area, and withdrawing of forest, shrubland and grassland during the 1985-2015 periods, which are expected to continue in the 2030 and 2045 periods. The LULC changes, which had occurred during the period of 1985 to 2015, had increased the annual flow (2.2%), wet seasonal flow (4.6%), surface runoff (9.3%) and water yield (2.4%). Conversely, the observed changes had reduced dry season flow (2.8%), lateral flow (5.7%), groundwater flow (7.8%) and ET (0.3%). The 2030 and 2045 LULC states are expected to further increase the annual and wet season flow, surface runoff and water yield, and reduce dry season flow, groundwater flow, lateral flow and ET. The change in hydrological components is a direct result of the significant transition from the vegetation to non-vegetation cover in the watershed. This suggests an urgent need to regulate the LULC in order to maintain the hydrological balance. Copyright © 2017 Elsevier B.V. All rights reserved.

Hydrodynamic behavior in the outer shear layer of partly obstructed open channels

NASA Astrophysics Data System (ADS)

Ben Meftah, Mouldi; De Serio, Francesca; Mossa, Michele

2014-06-01

Despite the many studies on flow in partly obstructed open channels, this issue remains of fundamental importance in order to better understand the interaction between flow behavior and the canopy structure. In the first part of this study we suggest a new theoretical approach able to model the flow pattern within the shear layer in the unobstructed domain, adjacent to the canopy area. Differently from previous studies, the new analytical solution of flow momentum equations takes into account the transversal velocity component of the flow, which is modelled as a linear function of the streamwise velocity. The proposed theoretical model is validated by different experiments carried out on a physical model of a very large rectangular channel by the research group of the Department of Civil, Environmental, Building Engineering and Chemistry of the Technical University of Bari. An array of vertical, rigid, and circular steel cylinders was partially mounted on the bottom in the central part of the flume, leaving two lateral areas of free flow circulation near the walls. The three-dimensional flow velocity components were measured using a 3D Acoustic Doppler Velocimeter. A comparison of the measured and predicted data of the present study with those obtained in other previous studies, carried out with different canopy density, show a non-dependence of this analytical solution on the array density and the Reynolds number. In the second part of the paper, detailed observations of turbulent intensities and spanwise Reynolds stresses in the unobstructed flow are analyzed and discussed. Differently from some earlier studies, it was observed that the peak of the turbulence intensity and that of the spanwise Reynolds stress are significantly shifted toward the center of the shear layer.

Development and testing of bio-inspired microelectromechanical pressure sensor arrays for increased situational awareness for marine vehicles

NASA Astrophysics Data System (ADS)

Dusek, J.; Kottapalli, A. G. P.; Woo, M. E.; Asadnia, M.; Miao, J.; Lang, J. H.; Triantafyllou, M. S.

2013-01-01

The lateral line found on most species of fish is a sensory organ without analog in humans. Using sensory feedback from the lateral line, fish are able to track prey, school, avoid obstacles, and detect vortical flow structures. Composed of both a superficial component, and a component contained within canals beneath the fish’s skin, the lateral line acts in a similar fashion to an array of differential pressure sensors. In an effort to enhance the situational and environmental awareness of marine vehicles, lateral-line-inspired pressure sensor arrays were developed to mimic the enhanced sensory capabilities observed in fish. Three flexible and waterproof pressure sensor arrays were fabricated for use as a surface-mounted ‘smart skin’ on marine vehicles. Two of the sensor arrays were based around the use of commercially available piezoresistive sensor dies, with innovative packaging schemes to allow for flexibility and underwater operation. The sensor arrays employed liquid crystal polymer and flexible printed circuit board substrates with metallic circuits and silicone encapsulation. The third sensor array employed a novel nanocomposite material set that allowed for the fabrication of a completely flexible sensor array. All three sensors were surface mounted on the curved hull of an autonomous kayak vehicle, and tested in both pool and reservoir environments. Results demonstrated that all three sensors were operational while deployed on the autonomous vehicle, and provided an accurate means for monitoring the vehicle dynamics.

Imaging dipole flow sources using an artificial lateral-line system made of biomimetic hair flow sensors

PubMed Central

Dagamseh, Ahmad; Wiegerink, Remco; Lammerink, Theo; Krijnen, Gijs

2013-01-01

In Nature, fish have the ability to localize prey, school, navigate, etc., using the lateral-line organ. Artificial hair flow sensors arranged in a linear array shape (inspired by the lateral-line system (LSS) in fish) have been applied to measure airflow patterns at the sensor positions. Here, we take advantage of both biomimetic artificial hair-based flow sensors arranged as LSS and beamforming techniques to demonstrate dipole-source localization in air. Modelling and measurement results show the artificial lateral-line ability to image the position of dipole sources accurately with estimation error of less than 0.14 times the array length. This opens up possibilities for flow-based, near-field environment mapping that can be beneficial to, for example, biologists and robot guidance applications. PMID:23594816

A high-resolution global-scale groundwater model

NASA Astrophysics Data System (ADS)

de Graaf, I. E. M.; Sutanudjaja, E. H.; van Beek, L. P. H.; Bierkens, M. F. P.

2015-02-01

Groundwater is the world's largest accessible source of fresh water. It plays a vital role in satisfying basic needs for drinking water, agriculture and industrial activities. During times of drought groundwater sustains baseflow to rivers and wetlands, thereby supporting ecosystems. Most global-scale hydrological models (GHMs) do not include a groundwater flow component, mainly due to lack of geohydrological data at the global scale. For the simulation of lateral flow and groundwater head dynamics, a realistic physical representation of the groundwater system is needed, especially for GHMs that run at finer resolutions. In this study we present a global-scale groundwater model (run at 6' resolution) using MODFLOW to construct an equilibrium water table at its natural state as the result of long-term climatic forcing. The used aquifer schematization and properties are based on available global data sets of lithology and transmissivities combined with the estimated thickness of an upper, unconfined aquifer. This model is forced with outputs from the land-surface PCRaster Global Water Balance (PCR-GLOBWB) model, specifically net recharge and surface water levels. A sensitivity analysis, in which the model was run with various parameter settings, showed that variation in saturated conductivity has the largest impact on the groundwater levels simulated. Validation with observed groundwater heads showed that groundwater heads are reasonably well simulated for many regions of the world, especially for sediment basins (R2 = 0.95). The simulated regional-scale groundwater patterns and flow paths demonstrate the relevance of lateral groundwater flow in GHMs. Inter-basin groundwater flows can be a significant part of a basin's water budget and help to sustain river baseflows, especially during droughts. Also, water availability of larger aquifer systems can be positively affected by additional recharge from inter-basin groundwater flows.

Compressible Flow Phenomena at Inception of Lateral Density Currents Fed by Collapsing Gas-Particle Mixtures

NASA Astrophysics Data System (ADS)

Valentine, Greg A.; Sweeney, Matthew R.

2018-02-01

Many geological flows are sourced by falling gas-particle mixtures, such as during collapse of lava domes, and impulsive eruptive jets, and sustained columns, and rock falls. The transition from vertical to lateral flow is complex due to the range of coupling between particles of different sizes and densities and the carrier gas, and due to the potential for compressible flow phenomena. We use multiphase modeling to explore these dynamics. In mixtures with small particles, and with subsonic speeds, particles follow the gas such that outgoing lateral flows have similar particle concentration and speed as the vertical flows. Large particles concentrate immediately upon impact and move laterally away as granular flows overridden by a high-speed jet of expelled gas. When a falling flow is supersonic, a bow shock develops above the impact zone, and this produces a zone of high pressure from which lateral flows emerge as overpressured wall jets. The jets form complex structures as the mixtures expand and accelerate and then recompress through a recompression zone that mimics a Mach disk shock in ideal gas jets. In mixtures with moderate to high ratios of fine to coarse particles, the latter tend to follow fine particles through the expansion-recompression flow fields because of particle-particle drag. Expansion within the flow fields can lead to locally reduced gas pressure that could enhance substrate erosion in natural flows. The recompression zones form at distances, and have peak pressures, that are roughly proportional to the Mach numbers of impacting flows.

Ecosystem processes at the watershed scale: hydrologic vegetation gradient as an indicator for lateral hydrologic connectivity of headwater catchments

Treesearch

Taehee Hwang; James M. Vose; Christina Tague

2012-01-01

Lateral water flow in catchments can produce important patterns in water and nutrient fluxes and stores and also influences the long-term spatial development of forest ecosystems. Specifically, patterns of vegetation type and density along hydrologic flow paths can represent a signal of the redistribution of water and nitrogen mediated by lateral hydrologic flow. This...

An evaluation of the hydrologic relevance of lateral flow in snow at hillslope and catchment scales

Treesearch

David Eiriksson; Michael Whitson; Charles H. Luce; Hans Peter Marshall; John Bradford; Shawn G. Benner; Thomas Black; Hank Hetrick; James P. McNamara

2013-01-01

Lateral downslope flow in snow during snowmelt and rain-on-snow (ROS) events is a well-known phenomenon, yet its relevance to water redistribution at hillslope and catchment scales is not well understood. We used dye tracers, geophysical methods, and hydrometric measurements to describe the snow properties that promote lateral flow, assess the relative velocities of...

Evaluation of the Separability of Monodisperse Polystyrene Latex Microspheres in a Continuous Flow Electrophoresis System

NASA Technical Reports Server (NTRS)

Williams, G., Jr.

1983-01-01

The continuous flow electrophoresis system makes electrophoresis possible in a free flowing film of aqueous electrolyte medium. The sample is introduced at one end of the chamber and is subjected to a lateral dc field. This process separates the sample into fractions since each component has a distinctive electrophoric mobility. Evaluations were made of sample conductivity and buffer conductivity as they affect sample band spread and separation using the Continuous Particle Electrophoresis (CPE) system. Samples were prepared from mixtures of 5 percent and 1 percent polystyrene latex (PSL) microspheres which were .4, .56 and .7 microns in diameter. These were prepared in electrolyte media 1x and 3x the conductivity of the curtain buffer, approximately 150 and 450 micro mhos/cm. Samples with matched conductivities produced greater resolution and less band spread than those with 3x the conductivity of the curtain buffer.

Review of literature on the finite-element solution of the equations of two-dimensional surface-water flow in the horizontal plane

USGS Publications Warehouse

Lee, Jonathan K.; Froehlich, David C.

1987-01-01

Published literature on the application of the finite-element method to solving the equations of two-dimensional surface-water flow in the horizontal plane is reviewed in this report. The finite-element method is ideally suited to modeling two-dimensional flow over complex topography with spatially variable resistance. A two-dimensional finite-element surface-water flow model with depth and vertically averaged velocity components as dependent variables allows the user great flexibility in defining geometric features such as the boundaries of a water body, channels, islands, dikes, and embankments. The following topics are reviewed in this report: alternative formulations of the equations of two-dimensional surface-water flow in the horizontal plane; basic concepts of the finite-element method; discretization of the flow domain and representation of the dependent flow variables; treatment of boundary conditions; discretization of the time domain; methods for modeling bottom, surface, and lateral stresses; approaches to solving systems of nonlinear equations; techniques for solving systems of linear equations; finite-element alternatives to Galerkin's method of weighted residuals; techniques of model validation; and preparation of model input data. References are listed in the final chapter.

A water tunnel flow visualization study of the vortex flow structures on the F/A-18 aircraft

NASA Technical Reports Server (NTRS)

Sandlin, Doral R.; Ramirez, Edgar J.

1991-01-01

The vortex flow structures occurring on the F/A-18 aircraft at high angles of attack were studied. A water tunnel was used to gather flow visualization data on the forebody vortex and the wing leading edge extension vortex. The longitudinal location of breakdown of the leading edge vortex was found to be consistently dependent on the angle of attack. Other parameters such as Reynolds number, model scale, and model fidelity had little influence on the overall behavior of the flow structures studied. The lateral location of the forebody vortex system was greatly influenced by changes in the angle of sideslip. Strong interactions can occur between the leading edge extension vortex and the forebody vortex. Close attention was paid to vortex induced flows on various airframe components of the F/A-18. Reynolds number and angle of attack greatly affected the swirling intensity, and therefore the strength of the studied vortices. Water tunnel results on the F/A-18 correlated well with those obtained in similar studies at both full and sub scale levels. The water tunnel can provide, under certain conditions, good simulations of realistic flows in full scale configurations.

Distributed flow estimation and closed-loop control of an underwater vehicle with a multi-modal artificial lateral line.

PubMed

DeVries, Levi; Lagor, Francis D; Lei, Hong; Tan, Xiaobo; Paley, Derek A

2015-03-25

Bio-inspired sensing modalities enhance the ability of autonomous vehicles to characterize and respond to their environment. This paper concerns the lateral line of cartilaginous and bony fish, which is sensitive to fluid motion and allows fish to sense oncoming flow and the presence of walls or obstacles. The lateral line consists of two types of sensing modalities: canal neuromasts measure approximate pressure gradients, whereas superficial neuromasts measure local flow velocities. By employing an artificial lateral line, the performance of underwater sensing and navigation strategies is improved in dark, cluttered, or murky environments where traditional sensing modalities may be hindered. This paper presents estimation and control strategies enabling an airfoil-shaped unmanned underwater vehicle to assimilate measurements from a bio-inspired, multi-modal artificial lateral line and estimate flow properties for feedback control. We utilize potential flow theory to model the fluid flow past a foil in a uniform flow and in the presence of an upstream obstacle. We derive theoretically justified nonlinear estimation strategies to estimate the free stream flowspeed, angle of attack, and the relative position of an upstream obstacle. The feedback control strategy uses the estimated flow properties to execute bio-inspired behaviors including rheotaxis (the tendency of fish to orient upstream) and station-holding (the tendency of fish to position behind an upstream obstacle). A robotic prototype outfitted with a multi-modal artificial lateral line composed of ionic polymer metal composite and embedded pressure sensors experimentally demonstrates the distributed flow sensing and closed-loop control strategies.

An Inexpensive, Fast and Sensitive Quantitative Lateral Flow Magneto-Immunoassay for Total Prostate Specific Antigen

PubMed Central

Barnett, Jacqueline M.; Wraith, Patrick; Kiely, Janice; Persad, Raj; Hurley, Katrina; Hawkins, Peter; Luxton, Richard

2014-01-01

We describe the detection characteristics of a device the Resonant Coil Magnetometer (RCM) to quantify paramagnetic particles (PMPs) in immunochromatographic (lateral flow) assays. Lateral flow assays were developed using PMPs for the measurement of total prostate specific antigen (PSA) in serum samples. A detection limit of 0.8 ng/mL was achieved for total PSA using the RCM and is at clinically significant concentrations. Comparison of data obtained in a pilot study from the analysis of serum samples with commercially available immunoassays shows good agreement. The development of a quantitative magneto-immunoassay in lateral flow format for total PSA suggests the potential of the RCM to operate with many immunoassay formats. The RCM has the potential to be modified to quantify multiple analytes in this format. This research shows promise for the development of an inexpensive device capable of quantifying multiple analytes at the point-of-care using a magneto-immunoassay in lateral flow format. PMID:25587419

New Fpg probe chemistry for direct detection of recombinase polymerase amplification on lateral flow strips.

PubMed

Powell, Michael L; Bowler, Frank R; Martinez, Aurore J; Greenwood, Catherine J; Armes, Niall; Piepenburg, Olaf

2018-02-15

Rapid, cost-effective and sensitive detection of nucleic acids has the ability to improve upon current practices employed for pathogen detection in diagnosis of infectious disease and food testing. Furthermore, if assay complexity can be reduced, nucleic acid amplification tests could be deployed in resource-limited and home use scenarios. In this study, we developed a novel Fpg (Formamidopyrimidine DNA glycosylase) probe chemistry, which allows lateral flow detection of amplification in undiluted recombinase polymerase amplification (RPA) reactions. The prototype nucleic acid lateral flow chemistry was applied to a human genomic target (rs1207445), Campylobacter jejuni 16S rDNA and two genetic markers of the important food pathogen E. coli O157:H7. All four assays have an analytical sensitivity between 10 and 100 copies DNA per amplification. Furthermore, the assay is performed with fewer hands-on steps than using the current RPA Nfo lateral flow method as dilution of amplicon is not required for lateral flow analysis. Due to the simplicity of the workflow, we believe that the lateral flow chemistry for direct detection could be readily adapted to a cost-effective single-use consumable, ideal for use in non-laboratory settings. Copyright © 2017. Published by Elsevier Inc.

A closed-form solution for steady-state coupled phloem/xylem flow using the Lambert-W function.

PubMed

Hall, A J; Minchin, P E H

2013-12-01

A closed-form solution for steady-state coupled phloem/xylem flow is presented. This incorporates the basic Münch flow model of phloem transport, the cohesion model of xylem flow, and local variation in the xylem water potential and lateral water flow along the transport pathway. Use of the Lambert-W function allows this solution to be obtained under much more general and realistic conditions than has previously been possible. Variation in phloem resistance (i.e. viscosity) with solute concentration, and deviations from the Van't Hoff expression for osmotic potential are included. It is shown that the model predictions match those of the equilibrium solution of a numerical time-dependent model based upon the same mechanistic assumptions. The effect of xylem flow upon phloem flow can readily be calculated, which has not been possible in any previous analytical model. It is also shown how this new analytical solution can handle multiple sources and sinks within a complex architecture, and can describe competition between sinks. The model provides new insights into Münch flow by explicitly including interactions with xylem flow and water potential in the closed-form solution, and is expected to be useful as a component part of larger numerical models of entire plants. © 2013 John Wiley & Sons Ltd.

Influence of lateral groundwater flow in a shallow aquifer on eco-hydrological process in a shrub-grass coexistence semiarid area

NASA Astrophysics Data System (ADS)

Wang, Siru; Sun, Jinhua; Lei, Huimin; Zhu, Qiande; Jiang, Sanyuan

2017-04-01

Topography has a considerable influence on eco-hydrological processes resulting from the patterns of solar radiation distribution and lateral water flow. However, not much quantitative information on the contribution of lateral groundwater flow on ecological processes such as vegetation growth and evapo-transpiration is available. To fill this gap, we used a simple eco-hydrological model based on water balance with a 3D groundwater module that uses Darcy's law. This model was applied to a non-contributing area of 50km2 dominated by grassland and shrubland with an underlying shallow aquifer. It was calibrated using manually and remotely sensed vegetation data and water flux data observed by eddy covariance system of two flux towers as well as water table data obtained from HOBO recorders of 40 wells. The results demonstrate that the maximum hydraulic gradient and the maximum flux of lateral groundwater flow reached to 0.156m m-1 and 0.093m3 s-1 respectively. The average annual maximum LAI in grassland, predominantly in low-lying areas, improved by about 5.9% while that in shrubland, predominantly in high-lying areas, remained the same when lateral groundwater flow is considered adequately compared to the case without considering lateral groundwater flow. They also show that LAI is positively and nonlinearly related to evapotranspiration, and that the greater the magnitude of evapotranspiration, the smaller the rate of increase of LAI. The results suggest that lateral groundwater flow should not be neglected when simulating eco-hydrological process in areas with a shallow aquifer.

Mapleson's Breathing Systems.

PubMed

Kaul, Tej K; Mittal, Geeta

2013-09-01

Mapleson breathing systems are used for delivering oxygen and anaesthetic agents and to eliminate carbon dioxide during anaesthesia. They consist of different components: Fresh gas flow, reservoir bag, breathing tubes, expiratory valve, and patient connection. There are five basic types of Mapleson system: A, B, C, D and E depending upon the different arrangements of these components. Mapleson F was added later. For adults, Mapleson A is the circuit of choice for spontaneous respiration where as Mapleson D and its Bains modifications are best available circuits for controlled ventilation. For neonates and paediatric patients Mapleson E and F (Jackson Rees modification) are the best circuits. In this review article, we will discuss the structure of the circuits and functional analysis of various types of Mapleson systems and their advantages and disadvantages.

Performance, Stability, and Control Investigation at Mach Numbers from 0.4 to 0.9 of a Model of the "Swallow" with Outer Wing Panels Swept 25 degree with and without Power Simulation

NASA Technical Reports Server (NTRS)

Runckel, Jack F.; Schmeer, James W.; Cassetti, Marlowe D.

1960-01-01

An investigation of the performance, stability, and control characteristics of a variable-sweep arrow-wing model (the "Swallow") with the outer wing panels swept 25 deg has been conducted in the Langley 16-foot transonic tunnel. The wing was uncambered and untwisted and had RAE 102 airfoil sections with a thickness-to-chord ratio of 0.14 normal to the leading edge. Four outboard engines located above and below the wing provided propulsive thrust, and, by deflecting in the pitch direction and rotating in the lateral plane, also produced control forces. A pair of swept lateral fins and a single vertical fin were mounted on each engine nacelle to provide aerodynamic stability and control. Jets-off data were obtained with flow-through nacelles, stimulating the effects of inlet flow; jet thrust and hot-jet interference effects were obtained with faired-nose nacelles housing hydrogen peroxide gas generators. Six-component force and moment data were obtained through a Mach number range of 0.40 to 0.90 at angles of attack and angles of sideslip from 0 deg to 15 deg. Longitudinal, directional, and lateral control were obtained by deflecting the nacelle-fin combinations as elevators, rudders, and ailerons at several fixed angles for each control.

A trench study to assess transfer of pesticides in subsurface lateral flow for a soil with contrasting texture on a sloping vineyard in Beaujolais.

PubMed

Peyrard, X; Liger, L; Guillemain, C; Gouy, V

2016-01-01

Subsurface lateral flow in both texture-contrast soils and catchments with shallow bedrock is suspected to be a non-point source of contamination of watercourses by pesticides used in agriculture. As a case study, the north of the Beaujolais region (eastern France) provides a favorable environment for such contamination due to its agro-pedo-climatic conditions. Environments seen in the Beaujolais region include intense viticulture, permeable and shallow soils, steep hillslopes, and storms that occur during the periods of pesticide application. Watercourse contamination by pesticides has been widely observed in this region, and offsite pesticide transport by subsurface lateral flow is suspected to be involved in diffuse and chronic presence of pesticides in surface water. In order to confirm and quantify the potential role of such processes in pesticide transfer, an automated trench system has been designed. The trench was set up on a steep farmed hillslope in a texture-contrast soil. It was equipped with a tipping bucket flow meter and an automatic sampler to monitor pesticide concentrations in lateral flow at fine resolution, by means of a flow-dependent sampling strategy. Four pesticides currently used in vine growing were studied to provide a range of mobility properties: one insecticide (chlorpyrifos-methyl) and three fungicides (spiroxamine, tebuconazole, and dimethomorph). With this system, it was possible to study pesticide concentration dynamics in the subsurface lateral flow, generated by substantial rainfall events following pesticide applications. The experimental design ascertained to be a suitable method in which to monitor subsurface lateral flow and related transfer of pesticides.

Ultra low-cost, portable smartphone optosensors for mobile point-of-care diagnostics

NASA Astrophysics Data System (ADS)

Wang, Li-Ju; Chang, Yu-Chung; Sun, Rongrong; Li, Lei

2018-02-01

Smartphone optosensors with integrated optical components make mobile point-of-care (MPoC) diagnostics be done near patients' side. It'll especially have a significant impact on healthcare delivery in rural or remote areas. Current FDA-approved PoC devices achieving clinical level are still at high cost and not affordable in rural hospitals. We present a series of ultra low-cost smartphone optical sensing devices for mobile point-of-care diagnosis. Aiming different targeting analytes and sensing mechanisms, we developed custom required optical components for each smartphone optosensros. These optical devices include spectrum readers, colorimetric readers for microplate, lateral flow device readers, and chemiluminescence readers. By integrating our unique designed optical components into smartphone optosening platform, the anlaytes can be precisely detected. Clinical testing results show the clinical usability of our smartphone optosensors. Ultra low-cost portable smartphone optosensors are affordable for rural/remote doctors.

Man-made flows from a fish's perspective: autonomous classification of turbulent fishway flows with field data collected using an artificial lateral line.

PubMed

Tuhtan, Jeffrey A; Fuentes-Perez, Juan Francisco; Toming, Gert; Schneider, Matthias; Schwarzenberger, Richard; Schletterer, Martin; Kruusmaa, Maarja

2018-05-25

The lateral line system provides fish with advanced mechanoreception over a wide range of flow conditions. Inspired by the abilities of their biological counterparts, artificial lateral lines have been developed and tested exclusively under laboratory settings. Motivated by the lack of flow measurements taken in the field which consider fluid-body interactions, we built a fish-shaped lateral line probe. The device is outfitted with 11 high-speed (2.5 kHz) time-synchronized pressure transducers, and designed to capture and classify flows in fish passage structures. A total of 252 field measurements, each with a sample size of 132 000 discrete sensor readings were recorded in the slots and across the pools of vertical slot fishways. These data were used to estimate the time-averaged flow velocity (R 2   =  0.952), which represents the most common metric to assess fishway flows. The significant contribution of this work is the creation and application of hydrodynamic signatures generated by the spatial distribution of pressure fluctuations on the fish-shaped body. The signatures are based on the collection of the pressure fluctuations' probability distributions, and it is shown that they can be used to automatically classify distinct flow regions within the pools of three different vertical slot fishways. For the first time, field data from operational fishway measurements are sampled and classified using an artificial lateral line, providing a completely new source of bioinspired flow information.

Evidence for lateral mantle plume flow feeding the Central Indian Ridge

NASA Astrophysics Data System (ADS)

Murton, B. J.; Tindle, A. G.

2003-04-01

The Central Indian Ridge exhibits morphological and geochemical features indicating lateral flow of shallow plume asthenosphere from the Reunion hot-spot to the ridge axis. South of the Marie Celeste fracture zone, at 18.25°S, the Central Indian Ridge is bound by a southward closing, “V”-shaped region of shallow crust that extends for over 800 km. Over this distance, the ridge axis deepens to the south and is also affected by left-stepping offsets that bring it towards the west. The northern end of the ridge, which is closest to the island of La'Réunion, is shallowest and dominated by an inflated segment with associated sheet flows covering over 50 square kilometres. These morphological features are usually associated with ridge-hot-spot interaction. However, the nearest active hot-spot lies over 1100 km to the west beneath the island of La'Réunion. Geochemical trends for basalts erupted along the Central Indian Ridge demonstrate a gradient of northward decreasing MgO and increasing SiO2, indicating a relationship between shallower crust and increased magmatic fractional crystallisation. Superimposed on this gradient is an excess increase in incompatible element ratios, indicative of mantle enrichment to the north. The enrichment correlates with the spreading-parallel distance between the ridge axis and the edge of the "V"-shaped region of anomalously shallow crust. Locally, the enriched mantle component is found preferentially at third-order ridge offsets and adjacent to the rift walls demonstrating melting of a compositionally stratified, spinel-lherzolite mantle. These features are evidence for shallow, lateral flow of enriched hot-spot asthenosphere at a velocity of ~333 mm yr-1 and with a flux of at least 50 m3 s-1, through a mantle 'worm', towards the ridge axis where it migrates south at a rate of 54 - 67 mm per year. The trend of the geochemical enrichment points to mixing between deeper N-MORB and shallower Reunion hot-spot sources beneath the Central Indian Ridge.

Nuclear reactor spacer grid and ductless core component

DOEpatents

Christiansen, David W.; Karnesky, Richard A.

1989-01-01

The invention relates to a nuclear reactor spacer grid member for use in a liquid cooled nuclear reactor and to a ductless core component employing a plurality of these spacer grid members. The spacer grid member is of the egg-shell type and is constructed so that the walls of the cell members of the grid member are formed of a single thickness of metal to avoid tolerance problems. Within each cell member is a hydraulic spring which laterally constrains the nuclear material bearing rod which passes through each cell member against a hardstop in response to coolant flow through the cell member. This hydraulic spring is also suitable for use in a water cooled nuclear reactor. A core component constructed of, among other components, a plurality of these spacer grid members, avoids the use of a full length duct by providing spacer sleeves about the sodium tubes passing through the spacer grid members at locations between the grid members, thereby maintaining a predetermined space between adjacent grid members.

Aerodynamic Characteristics of Two Waverider-Derived Hypersonic Cruise Configurations

NASA Technical Reports Server (NTRS)

Cockrell, Charles E., Jr.; Huebner, Lawrence D.; Finley, Dennis B.

1996-01-01

An evaluation was made on the effects of integrating the required aircraft components with hypersonic high-lift configurations known as waveriders to create hypersonic cruise vehicles. Previous studies suggest that waveriders offer advantages in aerodynamic performance and propulsion/airframe integration (PAI) characteristics over conventional non-waverider hypersonic shapes. A wind-tunnel model was developed that integrates vehicle components, including canopies, engine components, and control surfaces, with two pure waverider shapes, both conical-flow-derived waveriders for a design Mach number of 4.0. Experimental data and limited computational fluid dynamics (CFD) solutions were obtained over a Mach number range of 1.6 to 4.63. The experimental data show the component build-up effects and the aerodynamic characteristics of the fully integrated configurations, including control surface effectiveness. The aerodynamic performance of the fully integrated configurations is not comparable to that of the pure waverider shapes, but is comparable to previously tested hypersonic models. Both configurations exhibit good lateral-directional stability characteristics.

Multiplexed lateral flow biosensors: Technological advances for radically improving point-of-care diagnoses.

PubMed

Li, Jia; Macdonald, Joanne

2016-09-15

Lateral flow biosensors are a leading technology in point-of-care diagnostics due to their simplicity, rapidness and low cost. Their primacy in this arena continues through technological breakthroughs such as multiplexing: the detection of more than one biomarker in a single assay. Multiplexing capacity is critical for improving diagnostic efficiency, enhancing the diagnostic precision for specific diseases and reducing diagnostic cost. Here we review, for the first time, the various types and strategies employed for creating multiplexed lateral flow biosensors. These are classified into four main categories in terms of specific application or multiplexing level, namely linear, parameter, spatial and conceptual. We describe the practical applications and implications for each approach and compare their advantages and disadvantages. Importantly, multiplexing is still subject to limitations of the traditional lateral flow biosensor, such as sensitivity and specificity. However, by pushing the limitations of the traditional medium into the multiplex arena, several technological breakthroughs are emerging with novel solutions that further expand the utility of lateral flow biosensing for point-of-care applications. Copyright © 2016 Elsevier B.V. All rights reserved.

Subsonic investigations of vortex interaction control for enhanced high-alpha aerodynamics of a chine forebody/Delta wing configuration

NASA Technical Reports Server (NTRS)

Rao, Dhanvada M.; Bhat, M. K.

1992-01-01

A proposed concept to alleviate high alpha asymmetry and lateral/directional instability by decoupling of forebody and wing vortices was studied on a generic chine forebody/ 60 deg. delta configuration in the NASA Langley 7 by 10 foot High Speed Tunnel. The decoupling technique involved inboard leading edge flaps of varying span and deflection angle. Six component force/moment characteristics, surface pressure distributions and vapor-screen flow visualizations were acquired, on the basic wing-body configuration and with both single and twin vertical tails at M sub infinity = 0.1 and 0.4, and in the range alpha = 0 to 50 deg and beta = -10 to +10 degs. Results are presented which highlight the potential of vortex decoupling via leading edge flaps for enhanced high alpha lateral/directional characteristics.

Hydrology of two slopes in subarctic Yukon, Canada

NASA Astrophysics Data System (ADS)

Carey, Sean K.; Woo, Ming-Ko

1999-11-01

Two subarctic forested slopes in central Wolf Creek basin, Yukon, were studied in 1996-1997 to determine the seasonal pattern of the hydrologic processes. A south-facing slope has a dense aspen forest on silty soils with seasonal frost only and a north-facing slope has open stands of black spruce and an organic layer on top of clay sediments with permafrost. Snowmelt is advanced by approximately one month on the south-facing slope due to greater radiation receipt. Meltwater infiltrates its seasonally frozen soil with low ice content, recharging the soil moisture reservoir but yielding no lateral surface or subsurface flow. Summer evaporation depletes this recharged moisture and any additional rainfall input, at the expense of surface or subsurface flow. The north-facing slope with an ice rich substrate hinders deep percolation. Snow meltwater is impounded within the organic layer to produce surface runoff in rills and gullies, and subsurface flow along pipes and within the matrix of the organic soil. During the summer, most subsurface flows are confined to the organic layer which has hydraulic conductivities orders of magnitudes larger than the underlying boulder-clay. Evaporation on the north-facing slope declines as both the frost table and the water table descend in the summer. A water balance of the two slopes demonstrates that vertical processes of infiltration and evaporation dominate moisture exchanges on the south-facing slope, whereas the retardation of deep drainage by frost and by clayey soil on the permafrost slope promotes a strong lateral flow component, principally within the organic layer. These results have the important implication that permafrost slopes and organic horizons are the principal controls on streamflow generation in subarctic catchments.

A Paper and Plastic Device for Performing Recombinase Polymerase Amplification of HIV DNA

PubMed Central

Rohrman, Brittany A.; Richards-Kortum, Rebecca R.

2013-01-01

Despite the importance of early diagnosis and treatment of HIV, only a small fraction of HIV-exposed infants in low- and middle-income countries are tested for the disease. The gold standard for early infant diagnosis, DNA PCR, requires resources that are unavailable in poor settings, and no point-of-care HIV DNA test is currently available. We have developed a device constructed of layers of paper, glass fiber, and plastic that is capable of performing isothermal, enzymatic amplification of HIV DNA. The device is inexpensive, small, light-weight, and easy to assemble. The device stores lyophilized enzymes, facilitates mixing of reaction components, and supports recombinase polymerase amplification in five steps of operation. Using commercially available lateral flow strips as a detection method, we demonstrate the ability of our device to amplify 10 copies of HIV DNA to detectable levels in 15 minutes. Our results suggest that our device, which is designed to be used after DNA extraction from dried-blood spots, may serve in conjunction with lateral flow strips as part of a point-of-care HIV DNA test to be used in low resource settings. PMID:22733333

A paper and plastic device for performing recombinase polymerase amplification of HIV DNA.

PubMed

Rohrman, Brittany A; Richards-Kortum, Rebecca R

2012-09-07

Despite the importance of early diagnosis and treatment of HIV, only a small fraction of HIV-exposed infants in low- and middle-income countries are tested for the disease. The gold standard for early infant diagnosis, DNA PCR, requires resources that are unavailable in poor settings, and no point-of-care HIV DNA test is currently available. We have developed a device constructed of layers of paper, glass fiber, and plastic that is capable of performing isothermal, enzymatic amplification of HIV DNA. The device is inexpensive, small, light-weight, and easy to assemble. The device stores lyophilized enzymes, facilitates mixing of reaction components, and supports recombinase polymerase amplification in five steps of operation. Using commercially available lateral flow strips as a detection method, we demonstrate the ability of our device to amplify 10 copies of HIV DNA to detectable levels in 15 min. Our results suggest that our device, which is designed to be used after DNA extraction from dried-blood spots, may serve in conjunction with lateral flow strips as part of a point-of-care HIV DNA test to be used in low resource settings.

Xylem anisotropy and water transport--a model for the double sawcut experiment

Treesearch

Paul J. Schulte; David G. Costa

2010-01-01

Early experiments with overlapping cuts to the stems of trees demonstrated that lateral flow within the stem must be possible to allow such trees to maintain water flow to their leaves. We present a mathematical approach to considering lateral flow in stems by treating the xylem as an anisotropic medium for flow and develop an expression of its conductivity in the form...

Flow Generated by a Partially Penetrating Well in a Leaky Two-Aquifer System with a Storative Semiconfining Layer

NASA Astrophysics Data System (ADS)

Sepulveda, N.; Rohrer, K.

2008-05-01

The permeability of the semiconfining layers of the highly productive Floridan Aquifer System may be large enough to invalidate the assumptions of the leaky aquifer theory. These layers are the intermediate confining and the middle semiconfining units. The analysis of aquifer-test data with analytical solutions of the ground-water flow equation developed with the approximation of a low hydraulic conductivity ratio between the semiconfining layer and the aquifer may lead to inaccurate hydraulic parameters. An analytical solution is presented here for the flow in a confined leaky aquifer, the overlying storative semiconfining layer, and the unconfined aquifer, generated by a partially penetrating well in a two-aquifer system, and allowing vertical and lateral flow components to occur in the semiconfining layer. The equations describing flow caused by a partially penetrating production well are solved analytically to provide a method to accurately determine the hydraulic parameters in the confined aquifer, semiconfining layer, and unconfined aquifer from aquifer-test data. Analysis of the drawdown data from an aquifer test performed in central Florida showed that the flow solution presented here for the semiconfining layer provides a better match and a more unique identification of the hydraulic parameters than an analytical solution that considers only vertical flow in the semiconfining layer.

Mitigating the Hook Effect in Lateral Flow Sandwich Immunoassays Using Real-Time Reaction Kinetics.

PubMed

Rey, Elizabeth G; O'Dell, Dakota; Mehta, Saurabh; Erickson, David

2017-05-02

The quantification of analyte concentrations using lateral flow assays is a low-cost and user-friendly alternative to traditional lab-based assays. However, sandwich-type immunoassays are often limited by the high-dose hook effect, which causes falsely low results when analytes are present at very high concentrations. In this paper, we present a reaction kinetics-based technique that solves this problem, significantly increasing the dynamic range of these devices. With the use of a traditional sandwich lateral flow immunoassay, a portable imaging device, and a mobile interface, we demonstrate the technique by quantifying C-reactive protein concentrations in human serum over a large portion of the physiological range. The technique could be applied to any hook effect-limited sandwich lateral flow assay and has a high level of accuracy even in the hook effect range.

Battery operated preconcentration-assisted lateral flow assay.

PubMed

Kim, Cheonjung; Yoo, Yong Kyoung; Han, Sung Il; Lee, Junwoo; Lee, Dohwan; Lee, Kyungjae; Hwang, Kyo Seon; Lee, Kyu Hyoung; Chung, Seok; Lee, Jeong Hoon

2017-07-11

Paper-based analytical devices (e.g. lateral flow assays) are highly advantageous as portable diagnostic systems owing to their low costs and ease of use. Because of their low sensitivity and detection limits for biomolecules, these devices have several limitations in applications for real-field diagnosis. Here, we demonstrate a paper-based preconcentration enhanced lateral flow assay using a commercial β-hCG-based test. Utilizing a simple 9 V battery operation with a low power consumption of approximately 81 μW, we acquire a 25-fold preconcentration factor, demonstrating a clear sensitivity enhancement in the colorimetric lateral flow assay; consequently, clear colors are observed in a rapid kit test line, which cannot be monitored without preconcentration. This device can also facilitate a semi-quantitative platform using the saturation value and/or color intensity in both paper-based colorimetric assays and smartphone-based diagnostics.

Experimental Investigation Of Base Flow Buffeting On The Ariane 5 Launcher Using High Speed PIV

NASA Astrophysics Data System (ADS)

Schrijer, F. F. J.; Sciacchitano, A.; Scarrano, F.; Hannemann, K.; Pallegoix, J.-F.; Maseand, J. E. J.; Schwane, R.

2011-05-01

Experiments have been performed on a 1:60 scale Ariane 5 launcher in the DNW HST wind tunnel by means of two-component particle image velocimetry (2C-PIV). Measurements are performed for Mach 0.5 and Mach 0.8. The investigation focuses on studying the flow-buffeting phenomenon in the base of an Ariane V rocket. In total four configurations are tested: reference configuration, skirt, scoop and reference configuration without struts. It has been found that the presence of the struts has a large effect on the flow field; the secondary flow caused by the struts decreases the separated region and increases the overall turbulence. The skirt has the effect that the shear layer separates later and therefore does not reattach on the nozzle. Also in the separated region a secondary recirculation region is formed. For the scoop configuration it was observed that a second wake was formed by the scoop element causing the overall shear layer to become thicker. Finally using POD analysis two dominant modes are identified that can be associated to the separation bubble and shear layer dynamics.

Experimental modeling of gravity underflow in submarine channels

NASA Astrophysics Data System (ADS)

Islam, Mohammad Ashraful

Active and relic meandering channels are common on the seafloor adjacent to continental margins. These channels and their associated submarine fan deposits are products of the density-driven gravity flows known as turbidity currents. Unlike natural rivers, few attempts have been made to explore the process of channel meandering in the submarine environment. This research focuses on resolving the flow field of submarine channels by conducting experiments in a large laboratory basin. Saline and particulate density flows were studied in a straight channel, a single bend sinuous channel with vertical sidewalls and a multiple-bend sinuous channel with sloping sidewalls. Instantaneous velocities in steady developed currents were measured using 3-component acoustic Doppler velocity probes. Excess fractional density was measured at selected locations by collecting water sample using a siphon rake. Turbulent kinetic energy and Reynolds stress components are derived from the instantaneous velocity data of the straight channel experiments. Structure functions for mean velocity, Reynolds stress and turbulent kinetic energy profiles are derived by fitting normalized data. The normalized Reynolds-averaged velocity shows excellent similarity collapse while the Reynolds-stress and the turbulent kinetic energy profiles display reasonable similarity. Vertical profiles of the turbulent kinetic energy display two peaks separated by a zone of low turbulence; the ratio of the maximum to the depth-averaged turbulent kinetic energy is approximately 1.5. Theoretical profile of turbulent kinetic energy is derived. Comparisons of experimentally and theoretically derived turbulent kinetic energy profiles show reasonable agreement except at the position of velocity maximum where the theoretical profile displays a very small value. Velocity profiles derived from the measurements with confined flow in the single bend channel reveal that channel curvature drives two helical flow cells, one stacked upon the other. The lower cell forms near the channel bed surface and has a circulation pattern similar to fluvial channels where a near-bed flow is directed inward. The other circulation cell forms in the upper part of the gravity flow and has a streamwise vorticity opposite to the lower cell. The lower circulation cell can be reasonably approximated by open channel flow theory. The curvature induced mixing is found to shift the position of the maximum streamwise velocity in the upward direction. Experiments conducted in the multiple-bend channel reveals that the channel side slope does not alter the structure of the secondary flow as long as the flow remains confined within the channel. However, if flow spilling occurs at the channel bend, the lateral convection suppresses the upper circulation cell. The lateral slope promotes high superelevation of the dense-light fluid interface at a channel bend and the current almost entirely separates from the inner bank. Compared with the saline flow, the silt-laden flow has larger thickness and thus easily experiences spilling at the bend apex. The overbank flow approximately follows the pre-bend direction of the in-channel flow. Unlike the flow in the channel with vertical sidewalls, the maximum velocity position does not experience an upward shift. This may be attributed to the highly superelevated current interface. The saline flow experiences little reduction in flow velocity while the velocity of the particulate flow drops significantly in the downstream direction primarily due to in-channel sediment deposit.

Assessing the Impact of Land Use and Land Cover Change on Global Water Resources

NASA Astrophysics Data System (ADS)

Batra, N.; Yang, Y. E.; Choi, H. I.; Islam, A.; Charlotte, D. F.; Cai, X.; Kumar, P.

2007-12-01

Land use and land cover changes (LULCC) significantly modify the hydrological regime of the watersheds, affecting water resources and environment from regional to global scale. This study seeks to advance and integrate water and energy cycle observation, scientific understanding, and human impacts to assess future water availability. To achieve the research objective, we integrate and interpret past and current space based and in situ observations into a global hydrologic model (GHM). GHM is developed with enhanced spatial and temporal resolution, physical complexity, hydrologic theory and processes to quantify the impact of LULCC on physical variables: surface runoff, subsurface flow, groundwater, infiltration, ET, soil moisture, etc. Coupled with the common land model (CLM), a 3-dimensional volume averaged soil-moisture transport (VAST) model is expanded to incorporate the lateral flow and subgrid heterogeneity. The model consists of 11 soil-hydrology layers to predict lateral as well as vertical moisture flux transport based on Richard's equations. The primary surface boundary conditions (SBCs) include surface elevation and its derivatives, land cover category, sand and clay fraction profiles, bedrock depth and fractional vegetation cover. A consistent global GIS-based dataset is constructed for the SBCs of the model from existing observational datasets comprising of various resolutions, map projections and data formats. Global ECMWF data at 6-hour time steps for the period 1971 through 2000 is processed to get the forcing data which includes incoming longwave and shortwave radiation, precipitation, air temperature, pressure, wind components, boundary layer height and specific humidity. Land use land cover data, generated using IPCC scenarios for every 10 years from 2000 to 2100 is used for future assessment on water resources. Alterations due to LULCC on surface water balance components: ET, groundwater recharge and runoff are then addressed in the study. Land use change disrupts the hydrological cycle through increasing the water yield at some places leading to floods while diminishing, or even eliminating the low flow at other places.

A silicon dioxide modified magnetic nanoparticles-labeled lateral flow strips for HBs antigen.

PubMed

Zhang, Xueqing; Jiang, Lin; Zhang, Chunlei; Li, Ding; Wang, Can; Gao, Feng; Cui, Daxiang

2011-12-01

Herein we reported a new type of silicon dioxide wrapped magnetic nanoparticles-labeled lateral flow strip for detection of HBs antigen in sera. The SiO2 wrapped Fe3O4 nanocomposites were prepared and characterized by HR-TEM, FTIR and magnetometer. As-prepared nanocomposites were used to label anti-HBV surface monoclonal antibody, the lateral flow strips were constructed, and 100 specimens of sera were collected and tested. Results showed that the prepared SiO2 wrapped Fe3O4 nanocomposites were shell/core structure, well dispersed, with the size of 25 nm in diameter, the thickness of the shell was about 3 nm, their magnetic saturation intensity was 44.3 meu g(-1). Clinical sera specimens test results showed that the prepared lateral flow strips were with the detection limitation of 5 pg/mL by naked eye observation, and 0.1 pg/mL by CCD reader or MAR Analyzer, specificity was 100%. In conclusion, one kind of silicon dioxide wrapped magnetic nanoparticles-labeled lateral flow strip for ultrasensitive detection of HBs antigen was successfully developed, its ease of use, sensitiveness and low-cost make it well-suited for population-based on-the-site hepatitis B screening.

Relative Impacts of Low Permeability Subsurface Deposits on Recharge Basin Infiltration Rates

NASA Astrophysics Data System (ADS)

Oconnell, P.; Becker, M.; Pham, C.; Rodriguez, G.; Hutchinson, A.; Plumlee, M.

2017-12-01

Artificial recharge of aquifers through spreading basins has become an important component of water management in semi-arid climates. The rate at which water can be recharged in these basins is limited by the natural vertical permeability of the underlying deposits which may be highly variable both laterally and vertically. To help understand hydrostratigraphic controls on recharge, a newly constructed basin was surveyed and instrumented. Prior to flooding the basin, lithology was characterized by shallow hand coring, direct push coring, ground penetrating radar, and electrical resistivity. After flooding, recharge was monitored through piezometers, electrical resistivity, and a network of fiber optic distributed temperature sensing (DTS). The DTS network used temperature as a tracer to measure infiltration rate on 25 cm intervals both laterally and vertically. Several hundred paired DTS time series datasets (from fiber optic cables located at 0 and 0.5 meters below ground surface) were processed with the cross-wavelet transform (XWT) to calculate spatially and temporally continuous infiltration rates, which can be interpolated and animated to visualize heterogeneity. Time series data from 8-meter deep, vertically oriented DTS cables reveal depth intervals where infiltration rates vary. Inverted resistivity sections from repeated dipole-dipole surveys along the sidewall of a spreading basin exhibit a positive correlation with the distribution of relatively high and low infiltration rates, indicating zones of preferential downward (efficient) and lateral (inefficient) flow, respectively. In contrast to other monitored basins, no perching was observed in the vertically oriented DTS cables. The variation in recharge across the basin and the appearance of subsurface lateral flow can be explained in context of the alluvial depositional environment.

Balancing Aggradation and Progradation on a Vegetated Delta: The Importance of Fluctuating Discharge in Depositional Systems

NASA Astrophysics Data System (ADS)

Piliouras, Anastasia; Kim, Wonsuck; Carlson, Brandee

2017-10-01

Vegetation is an important component of constructional landscapes, as plants enhance deposition and provide organic sediment that can increase aggradation rates to combat land loss. We conducted two sets of laboratory experiments using alfalfa (Medicago sativa) to determine the effects of plants on channel organization and large-scale delta dynamics. In the first set, we found that rapid vegetation colonization enhanced deposition but inhibited channelization via increased form drag that reduced the shear stress available for sediment entrainment and transport. A second set of experiments used discharge fluctuations between flood and base flow (or interflood). Interfloods were critical for reworking the topset via channel incision and lateral migration to create channel relief and prevent rapid plant colonization. These low-flow periods also greatly reduced the topset slope in the absence of vegetation by removing topset sediment and delivering it to the shoreline. Floods decreased relief by filling channels with sediment, resulting in periods of rapid progradation and enhanced aggradation over the topset surface, which was amplified by vegetation. The combination of discharge fluctuations and vegetation thus provided a balance of vertical aggradation and lateral progradation. We conclude that plants can inhibit channelization in depositional systems and that discharge fluctuations encourage channel network organization to naturally balance against aggradation. Thus, variations in discharge are an important aspect of understanding the ecomorphodynamics of aggrading surfaces and modeling vegetated deltaic systems, and the combined influences of plants and discharge variations can act to balance vertical and lateral delta growth.

The influence of anisotropy on preferential flow in landslides

NASA Astrophysics Data System (ADS)

Cristiano, Elena; Barontini, Stefano; Bogaard, Thom A.; Shao, Wei

2015-04-01

Infiltration is one of the most important landslides triggering mechanisms and it is controlled by the hydraulic characteristics of the soil, which depends on the degree of saturation, the existence of preferential flow paths and by anisotropy. Many soils, indeed, exhibit a certain degree of anisotropy due to the stratification associated with soil forming process. Recently, various authors investigated the effect of rainfall in layered soils and its effect on rainfall triggered landslides by means of experimental, conceptual, numerical and theoretical approaches. However, the combined effect of anisotropy and preferential flow on infiltration process and related to rainfall induced landslides has, according to the authors best knowledge, not been studied yet. Aiming at better understanding the soil hydrological processes which take place during an infiltration process, the stability of a synthetic hill slope is numerically investigated. The geometry we considered for the model is a slope with two different layers: the upper soil layer consists of sandy loam, while the lower soil layer is made out of clay. The geometry was studied using both a single permeability and a dual permeability model. In the first case the hydraulic conductivity at saturation was considered isotropic, equal in all directions. Then the vertical component of the hydraulic conductivity tensor at saturation was reduced, while in the third scenario the horizontal component was reduced. In this way the anisotropy effects on both the principal directions were studied. In the dual permeability model, the influence of the anisotropy was considered only in the preferential flow domain, and the hydraulic conductivity at saturation of the soil matrix domain was defined as being isotropic. In order to evaluate also the effects of rainfall intensity on the slope, two different rainfall events were studied: a low intensity rainfall with a long time duration (2 mmh-1,150 h) and an high intensity rainfall with a short duration (20 mmh-1,15 h). The results show that the anisotropy facilitates the saturation process in the slope and that the vertical component of the soil water flow is set especially in the soil matrix domain, while the lateral component dominates in the preferential flow domain. In some scenarios the patterns of the water content in the unsaturated soil layers suggest the possibility of the onset of a perched water table.

Mean Flow and Turbulence Characteristics of a Full-Scale Spiral Corrugated Culvert with Implications to Fish Passage

DOE Office of Scientific and Technical Information (OSTI.GOV)

Richmond, Marshall C.; Deng, Zhiqun; Guensch, Gregory R.

2007-08-01

A micro-Acoustic Doppler Velocimeter (ADV) was used to differentiate certain three-dimensional velocity and turbulence characteristics in a full-scale culvert with spiral corrugations. The culvert was set up in a test bed constructed to examine juvenile salmon passage success in various culvert types. The test culvert was 12.2 m long and 1.83m in diameter and set at a 1.14% slope. The corrugations were 2.54 cm deep by 7.62 cm peak to peak with a 5° right handed pitch. Tailwater elevation was adjustable with a stop-log system and was set slightly above the water surface level at the culvert exit. Cross-sectional gridsmore » of ADV measurements were taken at discharges of 28, 57, 113, 227, and 453 lps at 9 locations within the culvert barrel and just inside the headwater and tailwater tanks. Results indicated that the spiral corrugations caused asymmetries in the velocity and turbulence distributions creating a Reduced Velocity Zone (RVZ) on the right side of the culvert as seen looking upstream, which small fish could utilize to aide their upstream passage. Velocity and turbulence magnitudes in the RVZ were found to be less than in mid-channel or on the left of the culvert, and the difference became greater at increased flow rates. In addition, lateral and vertical velocity components within the RVZ were small relative to the downstream axial component, while lateral and vertical turbulence intensities were comparable to the axial component. Vorticity was found to be largest in amplitude near the culvert walls, and changed sign left of the culvert centerline. Inlet loss coefficients were calculated as well and ranged from 0.32 to 0.42. Relationships between velocity and turbulence intensity in the RVZ and average velocity were developed, which may be useful for evaluating the conditions under which the barrel of a culvert is passable for juvenile fish.« less

Rolling with the flow: bumblebees flying in unsteady wakes.

PubMed

Ravi, Sridhar; Crall, James D; Fisher, Alex; Combes, Stacey A

2013-11-15

Our understanding of how variable wind in natural environments affects flying insects is limited because most studies of insect flight are conducted in either smooth flow or still air conditions. Here, we investigate the effects of structured, unsteady flow (the von Karman vortex street behind a cylinder) on the flight performance of bumblebees (Bombus impatiens). Bumblebees are 'all-weather' foragers and thus frequently experience variable aerial conditions, ranging from fully mixed, turbulent flow to unsteady, structured vortices near objects such as branches and stems. We examined how bumblebee flight performance differs in unsteady versus smooth flow, as well as how the orientation of unsteady flow structures affects their flight performance, by filming bumblebees flying in a wind tunnel under various flow conditions. The three-dimensional flight trajectories and orientations of bumblebees were quantified in each of three flow conditions: (1) smooth flow, (2) the unsteady wake of a vertical cylinder (inducing strong lateral disturbances) and (3) the unsteady wake of a horizontal cylinder (inducing strong vertical disturbances). In both unsteady conditions, bumblebees attenuated the disturbances induced by the wind quite effectively, but still experienced significant translational and rotational fluctuations as compared with flight in smooth flow. Bees appeared to be most sensitive to disturbance along the lateral axis, displaying large lateral accelerations, translations and rolling motions in response to both unsteady flow conditions, regardless of orientation. Bees also displayed the greatest agility around the roll axis, initiating voluntary casting maneuvers and correcting for lateral disturbances mainly through roll in all flow conditions. Both unsteady flow conditions reduced the upstream flight speed of bees, suggesting an increased cost of flight in unsteady flow, with potential implications for foraging patterns and colony energetics in natural, variable wind environments.

How bumblebees use lateral and ventral optic flow cues for position control in environments of different proximity.

PubMed

Linander, Nellie; Baird, Emily; Dacke, Marie

2017-05-01

Flying insects frequently navigate through environments of different complexity. In this study, buff-tailed bumblebees (Bombus terrestris L.) were trained to fly along tunnels of different widths, from 60 to 240 cm. In tunnel widths of 60 and 120 cm, bumblebees control their lateral position by balancing the magnitude of translational optic flow experienced in the lateral visual field of each eye. In wider tunnels, bumblebees use translational optic flow cues in the ventral visual field to control their lateral position and to steer along straight tracks. Our results also suggest that bumblebees prefer to fly over surfaces that provide strong ventral optic flow cues, rather than over featureless ones. Together, these strategies allow bumblebees to minimize the risk of collision and to maintain relatively straight flight paths in a broad range of environments.

High-Speed Lateral Flow Strategy for a Fast Biosensing with an Improved Selectivity and Binding Affinity.

PubMed

Cho, Dong Guk; Yoo, Haneul; Lee, Haein; Choi, Yeol Kyo; Lee, Minju; Ahn, Dong June; Hong, Seunghun

2018-05-10

We report a high-speed lateral flow strategy for a fast biosensing with an improved selectivity and binding affinity even under harsh conditions. In this strategy, biosensors were fixed at a location away from the center of a round shape disk, and the disk was rotated to create the lateral flow of a target solution on the biosensors during the sensing measurements. Experimental results using the strategy showed high reaction speeds, high binding affinity, and low nonspecific adsorptions of target molecules to biosensors. Furthermore, binding affinity between target molecules and sensing molecules was enhanced even in harsh conditions such as low pH and low ionic strength conditions. These results show that the strategy can improve the performance of conventional biosensors by generating high-speed lateral flows on a biosensor surface. Therefore, our strategy can be utilized as a simple but powerful tool for versatile bio and medical applications.

A Lateral Flow Biosensor for the Detection of Single Nucleotide Polymorphisms.

PubMed

Zeng, Lingwen; Xiao, Zhuo

2017-01-01

A lateral flow biosensor (LFB) is introduced for the detection of single nucleotide polymorphisms (SNPs). The assay is composed of two steps: circular strand displacement reaction and lateral flow biosensor detection. In step 1, the nucleotide at SNP site is recognized by T4 DNA ligase and the signal is amplified by strand displacement DNA polymerase, which can be accomplished at a constant temperature. In step 2, the reaction product of step 1 is detected by a lateral flow biosensor, which is a rapid and cost effective tool for nuclei acid detection. Comparing with conventional methods, it requires no complicated machines. It is suitable for the use of point of care diagnostics. Therefore, this simple, cost effective, robust, and promising LFB detection method of SNP has great potential for the detection of genetic diseases, personalized medicine, cancer related mutations, and drug-resistant mutations of infectious agents.

Lateral separation of colloids or cells by dielectrophoresis augmented by AC electroosmosis.

PubMed

Zhou, Hao; White, Lee R; Tilton, Robert D

2005-05-01

Colloidal particles and biological cells are patterned and separated laterally adjacent to a micropatterned electrode array by applying AC electric fields that are principally oriented normally to the electrode array. This is demonstrated for yeast cells, red blood cells, and colloidal polystyrene particles of different sizes and zeta-potentials. The separation mechanism is observed experimentally to depend on the applied field frequency and voltage. At high frequencies, particles position themselves in a manner that is consistent with dielectrophoresis, while at low frequencies, the positioning is explained in terms of a strong coupling between gravity, the vertical component of the dielectrophoretic force, and the Stokes drag on particles induced by AC electroosmotic flow. Compared to high frequency dielectrophoretic separations, the low frequency separations are faster and require lower applied voltages. Furthermore, the AC electroosmosis coupling with dielectrophoresis may enable cell separations that are not feasible based on dielectrophoresis alone.

The calculation of pressure on slender airplanes in subsonic and supersonic flow

NASA Technical Reports Server (NTRS)

Heaslet, Max A; Lomas, Harvard

1954-01-01

Under the assumption that a wing, body, or wing-body combination is slender or flying at near sonic velocity, expressions are given which permit the calculation of pressure in the immediate vicinity of the configuration. The disturbance field, in both subsonic and supersonic flight, is shown to consist of two-dimensional disturbance fields extending laterally and a longitudinal field that depends on the streamwise growth of cross-sectional area. A discussion is also given of couplings, between lifting and thickness effects, that necessarily arise as a result of the quadratic dependence of pressure on the induced velocity components. (author)

A detached eddy simulation model for the study of lateral separation zones along a large canyon-bound river

NASA Astrophysics Data System (ADS)

Alvarez, Laura V.; Schmeeckle, Mark W.; Grams, Paul E.

2017-01-01

Lateral flow separation occurs in rivers where banks exhibit strong curvature. In canyon-bound rivers, lateral recirculation zones are the principal storage of fine-sediment deposits. A parallelized, three-dimensional, turbulence-resolving model was developed to study the flow structures along lateral separation zones located in two pools along the Colorado River in Marble Canyon. The model employs the detached eddy simulation (DES) technique, which resolves turbulence structures larger than the grid spacing in the interior of the flow. The DES-3D model is validated using Acoustic Doppler Current Profiler flow measurements taken during the 2008 controlled flood release from Glen Canyon Dam. A point-to-point validation using a number of skill metrics, often employed in hydrological research, is proposed here for fluvial modeling. The validation results show predictive capabilities of the DES model. The model reproduces the pattern and magnitude of the velocity in the lateral recirculation zone, including the size and position of the primary and secondary eddy cells, and return current. The lateral recirculation zone is open, having continuous import of fluid upstream of the point of reattachment and export by the recirculation return current downstream of the point of separation. Differences in magnitude and direction of near-bed and near-surface velocity vectors are found, resulting in an inward vertical spiral. Interaction between the recirculation return current and the main flow is dynamic, with large temporal changes in flow direction and magnitude. Turbulence structures with a predominately vertical axis of vorticity are observed in the shear layer becoming three-dimensional without preferred orientation downstream.

Does lateral versus medial exposure influence total knee tibial component final external rotation? A CT based study.

PubMed

Passeron, D; Gaudot, F; Boisrenoult, P; Fallet, L; Beaufils, P

2009-10-01

A previous study demonstrated that performing a total knee arthroplasty through a lateral approach including anterior tibial tuberosity (ATT) osteotomy (refixed in its original position) presented numerous advantages: correcting the preoperative patella lateral tilt and improving postoperative patella tracking. We hypothesized that these improvements in patella centering were, at least in part, due to an increased external rotation of the tibial component. Postoperative scannographic studies were, therefore, undertaken to measure tibial component rotation and analyze the results according the medial and lateral exposure used. Rotational positioning of the tibial component is influenced by the lateral or medial approach selected at surgery. Forty-five CAT scans, performed according to the protocol criteria of the French Hip and Knee Society (SFHG), were studied 3 months postoperatively: 15 knees operated through the lateral approach and 30 knees operated through a standard medial approach. The total knee utilized in all these cases was a posteriorly stabilized, fixed-bearing, design. We measured first the angle formed between the perpendicular to the transverse axis of the tibial component and the axis joining the ATT to the center of the knee; second we also measured the coronal distance between the center of the component and the anterior tibial tuberosity (ATT). In the group using the medial approach, the lateral position of the ATT was 7 + or - 3mm with a rotation angle of 18 degrees . In the group using the lateral approach these measurements were respectively 1 + or - 4mm and 2 degrees (p<0.0001). External rotation of the tibial component is substantially increased by the lateral approach compared to the medial approach. Better exposure of the lateral tibial plateau is probably responsible of this difference. This increased external rotation improves postoperative patella tracking. Prospective; comparative; non-randomized study; level 3. 2009 Elsevier Masson SAS. All rights reserved.

Lateral fluid flow in a compacting sand-shale sequence: South Caspian basin.

USGS Publications Warehouse

Bredehoeft, J.D.; Djevanshir, R.D.; Belitz, K.R.

1988-01-01

The South Caspian basin contains both sands and shales that have pore-fluid pressures substantially in excess of hydrostatic fluid pressure. Pore-pressure data from the South Caspian basin demonstrate that large differences in excess hydraulic head exist between sand and shale. The data indicate that sands are acting as drains for overlying and underlying compacting shales and that fluid flows laterally through the sand on a regional scale from the basin interior northward to points of discharge. The major driving force for the fluid movement is shale compaction. We present a first- order mathematical analysis in an effort to test if the permeability of the sands required to support a regional flow system is reasonable. The results of the analysis suggest regional sand permeabilities ranging from 1 to 30 md; a range that seems reasonable. This result supports the thesis that lateral fluid flow is occurring on a regional scale within the South Caspian basin. If vertical conduits for flow exist within the basin, they are sufficiently impermeable and do not provide a major outlet for the regional flow system. The lateral fluid flow within the sands implies that the stratigraphic sequence is divided into horizontal units that are hydraulically isolated from one another, a conclusion that has important implications for oil and gas migration.-Authors

Rapid and Sensitive Detection of Protein Biomarker Using a Portable Fluorescence Biosensor based on Quantum Dots and a Lateral Flow Test Strip

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, Zhaohui; Wang, Ying; Wang, Jun

2010-08-15

A portable fluorescence biosensor with rapid and ultrasensitive response for trace protein has been built up with quantum dots and lateral flow test strip. The superior signal brightness and high photostability of quantum dots are combined with the promising advantages of lateral flow test strip and resulted in high sensitivity, selectivity and speedy for protein detection. Nitrated ceruloplasmin, a significant biomarker for cardiovascular disease, lung cancer and stress response to smoking, was used as model protein to demonstrate the good performances of this proposed Qdot-based lateral flow test strip. Quantitative detection of nitrated ceruloplasmin was realized by recording the fluorescencemore » intensity of quantum dots captured on the test line. Under optimal conditions, this portable fluorescence biosensor displays rapid responses for nitrated ceruloplasmin in wide dynamic range with a detection limit of 0.1ng/mL (S/N=3). Furthermore, the biosensor was successfully utilized for spiked human plasma sample detection with the concentration as low as 1ng/mL. The results demonstrate that the quantum dot-based lateral flow test strip is capable for rapid, sensitive, and quantitative detection of nitrated ceruloplasmin and hold a great promise for point-of-care and in field analysis of other protein biomarkers.« less

Simulating Water Flow in Variably Saturated Soils - Exploring the Advantage of Three-dimensional Models

NASA Astrophysics Data System (ADS)

Hopp, L.; Ivanov, V. Y.

2010-12-01

There is still a debate in rainfall-runoff modeling over the advantage of using three-dimensional models based on partial differential equations describing variably saturated flow vs. models with simpler infiltration and flow routing algorithms. Fully explicit 3D models are computationally demanding but allow the representation of spatially complex domains, heterogeneous soils, conditions of ponded infiltration, and solute transport, among others. Models with simpler infiltration and flow routing algorithms provide faster run times and are likely to be more versatile in the treatment of extreme conditions such as soil drying but suffer from underlying assumptions and ad-hoc parameterizations. In this numerical study, we explore the question of whether these two model strategies are competing approaches or if they complement each other. As a 3D physics-based model we use HYDRUS-3D, a finite element model that numerically solves the Richards equation for variably-saturated water flow. As an example of a simpler model, we use tRIBS+VEGGIE that solves the 1D Richards equation for vertical flow and applies Dupuit-Forchheimer approximation for saturated lateral exchange and gravity-driven flow for unsaturated lateral exchange. The flow can be routed using either the D-8 (steepest descent) or D-infinity flow routing algorithms. We study lateral subsurface stormflow and moisture dynamics at the hillslope-scale, using a zero-order basin topography, as a function of storm size, antecedent moisture conditions and slope angle. The domain and soil characteristics are representative of a forested hillslope with conductive soils in a humid environment, where the major runoff generating process is lateral subsurface stormflow. We compare spatially integrated lateral subsurface flow at the downslope boundary as well as spatial patterns of soil moisture. We illustrate situations where both model approaches perform equally well and identify conditions under which the application of a fully-explicit 3D model may be required for a realistic description of the hydrologic response.

Flow structures and sandbar dynamics in a canyon river during a controlled flood, Colorado River, Arizona

USGS Publications Warehouse

Wright, S.A.; Kaplinski, M.

2011-01-01

In canyon rivers, debris fan constrictions create rapids and downstream pools characterized by secondary flow structures that are closely linked to channel morphology. In this paper we describe detailed measurements of the three-dimensional flow structure and sandbar dynamics of two pools along the Colorado River in the Grand Canyon during a controlled flood release from Glen Canyon Dam. Results indicate that the pools are characterized by large lateral recirculation zones (eddies) resulting from flow separation downstream from the channel constrictions, as well as helical flow structures in the main channel and eddy. The lateral recirculation zones are low-velocity areas conducive to fine sediment deposition, particularly in the vicinity of the separation and reattachment points and are thus the dominant flow structures controlling sandbar dynamics. The helical flow structures also affect morphology but appear secondary in importance to the lateral eddies. During the controlled flood, sandbars in the separation and reattachment zones at both sites tended to build gradually during the rising limb and peak flow. Deposition in shallow water on the sandbars was accompanied by erosion in deeper water along the sandbar slope at the interface with the main channel. Erosion occurred via rapid mass failures as well as by gradual boundary shear stress driven processes. The flow structures and morphologic links at our study sites are similar to those identified in other river environments, in particular sharply curved meanders and channel confluences where the coexistence of lateral recirculation and helical flows has been documented. Copyright 2011 by the American Geophysical Union.

The 3D pore structure and fluid dynamics simulation of macroporous monoliths: High permeability due to alternating channel width.

PubMed

Jungreuthmayer, Christian; Steppert, Petra; Sekot, Gerhard; Zankel, Armin; Reingruber, Herbert; Zanghellini, Jürgen; Jungbauer, Alois

2015-12-18

Polymethacrylate-based monoliths have excellent flow properties. Flow in the wide channel interconnected with narrow channels is theoretically assumed to account for favorable permeability. Monoliths were cut into 898 slices in 50nm distances and visualized by serial block face scanning electron microscopy (SBEM). A 3D structure was reconstructed and used for the calculation of flow profiles within the monolith and for calculation of pressure drop and permeability by computational fluid dynamics (CFD). The calculated and measured permeabilities showed good agreement. Small channels clearly flowed into wide and wide into small channels in a repetitive manner which supported the hypothesis describing the favorable flow properties of these materials. This alternating property is also reflected in the streamline velocity which fluctuated. These findings were corroborated by artificial monoliths which were composed of regular (interconnected) cells where narrow cells followed wide cells. In the real monolith and the artificial monoliths with interconnected flow channels similar velocity fluctuations could be observed. A two phase flow simulation showed a lateral velocity component, which may contribute to the transport of molecules to the monolith wall. Our study showed that the interconnection of small and wide pores is responsible for the excellent pressure flow properties. This study is also a guide for further design of continuous porous materials to achieve good flow properties. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

Lateralization and Binaural Interaction of Middle-Latency and Late-Brainstem Components of the Auditory Evoked Response.

PubMed

Dykstra, Andrew R; Burchard, Daniel; Starzynski, Christian; Riedel, Helmut; Rupp, Andre; Gutschalk, Alexander

2016-08-01

We used magnetoencephalography to examine lateralization and binaural interaction of the middle-latency and late-brainstem components of the auditory evoked response (the MLR and SN10, respectively). Click stimuli were presented either monaurally, or binaurally with left- or right-leading interaural time differences (ITDs). While early MLR components, including the N19 and P30, were larger for monaural stimuli presented contralaterally (by approximately 30 and 36 % in the left and right hemispheres, respectively), later components, including the N40 and P50, were larger ipsilaterally. In contrast, MLRs elicited by binaural clicks with left- or right-leading ITDs did not differ. Depending on filter settings, weak binaural interaction could be observed as early as the P13 but was clearly much larger for later components, beginning at the P30, indicating some degree of binaural linearity up to early stages of cortical processing. The SN10, an obscure late-brainstem component, was observed consistently in individuals and showed linear binaural additivity. The results indicate that while the MLR is lateralized in response to monaural stimuli-and not ITDs-this lateralization reverses from primarily contralateral to primarily ipsilateral as early as 40 ms post stimulus and is never as large as that seen with fMRI.

Strategic Factors on Interpreting Remanufacturing Quality-Certifying Framework to Address Warranty Aftermarket for Malaysian Industry

NASA Astrophysics Data System (ADS)

Mohamed, N.; Saman, M. Z. M.; Sharif, S.; Hamzah, H. S.

2018-03-01

While the concept of remanufacturing is gaining popularity globally, literature and theory on strategic decision-making on certifying for warranty in this area remain limited. A strategic and establish concept flow is developed based on extensive literature review and surveys with experienced experts who are dealing with remanufactured, reconditioned, rebuilt and reused components. The remanufacturing research on evaluating quality assurance of remanufactured component targets macro-level parameters and the indicators which must be confirmed for evaluation. The strategic remanufacturing factors identified from the literature review are discussed in a brainstorming session with a number of remanufacturing researchers and academic experts. The study is further broadened by industrial surveys and case studies to justify the inputs on developing a framework to certify remanufactured components. Preliminary results have established the key factors of remanufacturing quality control that might lead to the strict quality assurance of remanufactured components. Later, the developed framework can be used as a benchmarking tool to certify remanufactured components and warranty issuance. The findings serve as the foundation for further research concerning Original Equipment Manufacturer (OEM) or Original Equipment Remanufacturer (OER) and Independent Equipment Remanufacturer (IER) in the Malaysian Remanufacturing Industry.

Flow patterns and bathymetric signatures on the delta front of a prograding river delta

NASA Astrophysics Data System (ADS)

Shaw, J.; Mohrig, D. C.; Wagner, R. W.

2016-02-01

The transition of water between laterally confined channels and the unchannelized delta front controls the growth pattern of river deltas, but is difficult to measure on field-scale deltas. We quantify flow patterns, bathymetry and bathymetric evolution for the subaqueous delta front on the Wax Lake Delta (WLD), a rapidly prograding delta in coastal Louisiana. The flow direction field, mapped using streaklines composed of biogenic slicks on the water surface, shows that a significant portion of flow ( 59%) departs subaqueous channels laterally over the subaqueous margins of the channel seaward of the shoreline. Synoptic datasets of bathymetry and flow direction allow spatial changes in flow velocity to be quantified. Most lateral flow divergence and deceleration occurs within 3-8 channel widths outboard of subaqueous channel margins, rather than downstream of channel tips. In interdistributary bays, deposit elevation decreases with a basinward slope of 2.4 x 10-4 with distance from a channel margin along any flow path. Flow patterns and this slope produce constructional features called interdistributary troughs - topographic lows in the center of interdistributary bays. These data show that flow patterns and bathymetry on the delta front are coupled both at the transition from channelized to unchannelized flow and in the depositional regions outside the distributary network.

Numerical determination of lateral loss coefficients for subchannel analysis in nuclear fuel bundles

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sin Kim; Goon-Cherl Park

1995-09-01

An accurate prediction of cross-flow based on detailed knowledge of the velocity field in subchannels of a nuclear fuel assembly is of importance in nuclear fuel performance analysis. In this study, the low-Reynolds number {kappa}-{epsilon} turbulence model has been adopted in two adjacent subchannels with cross-flow. The secondary flow is estimated accurately by the anisotropic algebraic Reynolds stress model. This model was numerically calculated by the finite element method and has been verified successfully through comparison with existing experimental data. Finally, with the numerical analysis of the velocity field in such subchannel domain, an analytical correlation of the lateral lossmore » coefficient is obtained to predict the cross-flow rate in subchannel analysis codes. The correlation is expressed as a function of the ratio of the lateral flow velocity to the donor subchannel axial velocity, recipient channel Reynolds number and pitch-to-diameter.« less

Repeatability of Doppler ultrasound measurements of hindlimb blood flow in halothane anaesthetised horses.

PubMed

Raisis, A L; Young, L E; Meire, H; Walsh, K; Taylor, P M; Lekeux, P

2000-05-01

The purpose of this study was to determine the repeatability of femoral blood flow recorded using Doppler ultrasound in anaesthetised horses. Doppler ultrasound of the femoral artery and vein was performed in 6 horses anaesthetised with halothane and positioned in left lateral recumbency. Velocity spectra, recorded using low pulse repetition frequency, were used to calculate time-averaged mean velocity (TAV), velocity of component a (TaVa), velocity of component b (TaVb), volumetric flow, early diastolic deceleration slope (EDDS) and pulsatility index (PI). Within-patient variability was determined for sequential Doppler measurements recorded during a single standardised anaesthetic episode. Within-patient variability was also determined for Doppler and cardiovascular measurements recorded during 4 separate standardised anaesthetic episodes performed at intervals of at least one month. Within-patient variation during a single anaesthetic episode was small. Coefficients of variation (cv) were <12.5% for arterial measurements and <17% for venous measurements. Intraclass correlation coefficient was >0.75 for all measurements. No significant change was observed in measurements of cardiovascular function suggesting that within-patient variation observed during a single anaesthetic episode was due to measurement error. In contrast, within-patient variation during 4 separate anaesthetic episodes was marked (cv>17%) for most Doppler measurements obtained from arteries and veins. Variation in measurements of cardiovascular function were marked (cv>20%), suggesting that there is marked biological variation in central and peripheral observed. Further studies are warranted to determine the ability of this technique to detect differences in blood flow during administration of different anaesthetic agents.

Automated Low-Cost Smartphone-Based Lateral Flow Saliva Test Reader for Drugs-of-Abuse Detection.

PubMed

Carrio, Adrian; Sampedro, Carlos; Sanchez-Lopez, Jose Luis; Pimienta, Miguel; Campoy, Pascual

2015-11-24

Lateral flow assay tests are nowadays becoming powerful, low-cost diagnostic tools. Obtaining a result is usually subject to visual interpretation of colored areas on the test by a human operator, introducing subjectivity and the possibility of errors in the extraction of the results. While automated test readers providing a result-consistent solution are widely available, they usually lack portability. In this paper, we present a smartphone-based automated reader for drug-of-abuse lateral flow assay tests, consisting of an inexpensive light box and a smartphone device. Test images captured with the smartphone camera are processed in the device using computer vision and machine learning techniques to perform automatic extraction of the results. A deep validation of the system has been carried out showing the high accuracy of the system. The proposed approach, applicable to any line-based or color-based lateral flow test in the market, effectively reduces the manufacturing costs of the reader and makes it portable and massively available while providing accurate, reliable results.

Twin tubular pinch effect in curving confined flows

PubMed Central

Clime, Liviu; Morton, Keith J.; Hoa, Xuyen D.; Veres, Teodor

2015-01-01

Colloidal suspensions of buoyancy neutral particles flowing in circular pipes focus into narrow distributions near the wall due to lateral migration effects associated with fluid inertia. In curving flows, these distributions are altered by Dean currents and the interplay between Reynolds and Dean numbers is used to predict equilibrium positions. Here, we propose a new description of inertial lateral migration in curving flows that expands current understanding of both focusing dynamics and equilibrium distributions. We find that at low Reynolds numbers, the ratio δ between lateral inertial migration and Dean forces scales simply with the particle radius, coil curvature and pipe radius as . A critical value δc = 0.148 of this parameter is identified along with two related inertial focusing mechanisms. In the regime below δc, coined subcritical, Dean forces generate permanently circulating, twinned annuli, each with intricate equilibrium particle distributions including eyes and trailing arms. At δ > δc (supercritical regime) inertial lateral migration forces are dominant and particles focus to a single stable equilibrium position. PMID:25927878

Experimental Investigation of the Low-Speed Aerodynamic Characteristics of a 5.8-Percent Scale Hybrid Wing Body Configuration

NASA Technical Reports Server (NTRS)

Gatlin, Gregory M.; Vicroy, Dan D.; Carter, Melissa B.

2012-01-01

A low-speed experimental investigation has been conducted on a 5.8-percent scale Hybrid Wing Body configuration in the NASA Langley 14- by 22-Foot Subsonic Tunnel. This Hybrid Wing Body (HWB) configuration was designed with specific intention to support the NASA Environmentally Responsible Aviation (ERA) Project goals of reduced noise, emissions, and fuel burn. This HWB configuration incorporates twin, podded nacelles mounted on the vehicle upper surface between twin vertical tails. Low-speed aerodynamic characteristics were assessed through the acquisition of force and moment, surface pressure, and flow visualization data. Longitudinal and lateral-directional characteristics were investigated on this multi-component model. The effects of a drooped leading edge, longitudinal flow-through nacelle location, vertical tail shape and position, elevon deflection, and rudder deflection have been studied. The basic configuration aerodynamics, as well as the effects of these configuration variations, are presented in this paper.

Mass independent kinetic energy reducing inlet system for vacuum environment

DOEpatents

Reilly, Peter T.A.

2014-05-13

A particle inlet system comprises a first chamber having a limiting orifice for an incoming gas stream and a micrometer controlled expansion slit. Lateral components of the momentum of the particles are substantially cancelled due to symmetry of the configuration once the laminar flow converges at the expansion slit. The particles and flow into a second chamber, which is maintained at a lower pressure than the first chamber, and then moves into a third chamber including multipole guides for electromagnetically confining the particle. The vertical momentum of the particles descending through the center of the third chamber is minimized as an upward stream of gases reduces the downward momentum of the particles. The translational kinetic energy of the particles is near-zero irrespective of the mass of the particles at an exit opening of the third chamber, which may be advantageously employed to provide enhanced mass resolution in mass spectrometry.

Mass independent kinetic energy reducing inlet system for vacuum environment

DOEpatents

Reilly, Peter T. A. [Knoxville, TN

2010-12-14

A particle inlet system comprises a first chamber having a limiting orifice for an incoming gas stream and a micrometer controlled expansion slit. Lateral components of the momentum of the particles are substantially cancelled due to symmetry of the configuration once the laminar flow converges at the expansion slit. The particles and flow into a second chamber, which is maintained at a lower pressure than the first chamber, and then moves into a third chamber including multipole guides for electromagnetically confining the particle. The vertical momentum of the particles descending through the center of the third chamber is minimized as an upward stream of gases reduces the downward momentum of the particles. The translational kinetic energy of the particles is near-zero irrespective of the mass of the particles at an exit opening of the third chamber, which may be advantageously employed to provide enhanced mass resolution in mass spectrometry.

Mass independent kinetic energy reducing inlet system for vacuum environment

DOEpatents

Reilly, Peter T.A.

2013-12-03

A particle inlet system comprises a first chamber having a limiting orifice for an incoming gas stream and a micrometer controlled expansion slit. Lateral components of the momentum of the particles are substantially cancelled due to symmetry of the configuration once the laminar flow converges at the expansion slit. The particles and flow into a second chamber, which is maintained at a lower pressure than the first chamber, and then moves into a third chamber including multipole guides for electromagnetically confining the particle. The vertical momentum of the particles descending through the center of the third chamber is minimized as an upward stream of gases reduces the downward momentum of the particles. The translational kinetic energy of the particles is near-zero irrespective of the mass of the particles at an exit opening of the third chamber, which may be advantageously employed to provide enhanced mass resolution in mass spectrometry.

Simulation and validation of concentrated subsurface lateral flow paths in an agricultural landscape

NASA Astrophysics Data System (ADS)

Zhu, Q.; Lin, H. S.

2009-08-01

The importance of soil water flow paths to the transport of nutrients and contaminants has long been recognized. However, effective means of detecting concentrated subsurface flow paths in a large landscape are still lacking. The flow direction and accumulation algorithm based on single-direction flow algorithm (D8) in GIS hydrologic modeling is a cost-effective way to simulate potential concentrated flow paths over a large area once relevant data are collected. This study tested the D8 algorithm for simulating concentrated lateral flow paths at three interfaces in soil profiles in a 19.5-ha agricultural landscape in central Pennsylvania, USA. These interfaces were (1) the interface between surface plowed layers of Ap1 and Ap2 horizons, (2) the interface with subsoil water-restricting clay layer where clay content increased to over 40%, and (3) the soil-bedrock interface. The simulated flow paths were validated through soil hydrologic monitoring, geophysical surveys, and observable soil morphological features. The results confirmed that concentrated subsurface lateral flow occurred at the interfaces with the clay layer and the underlying bedrock. At these two interfaces, the soils on the simulated flow paths were closer to saturation and showed more temporally unstable moisture dynamics than those off the simulated flow paths. Apparent electrical conductivity in the soil on the simulated flow paths was elevated and temporally unstable as compared to those outside the simulated paths. The soil cores collected from the simulated flow paths showed significantly higher Mn content at these interfaces than those away from the simulated paths. These results suggest that (1) the D8 algorithm is useful in simulating possible concentrated subsurface lateral flow paths if used with appropriate threshold value of contributing area and sufficiently detailed digital elevation model (DEM); (2) repeated electromagnetic surveys can reflect the temporal change of soil water storage and thus is a useful indicator of possible subsurface flow path over a large area; and (3) observable Mn distribution in soil profiles can be used as a simple indicator of water flow paths in soils and over the landscape; however, it does require sufficient soil sampling (by excavation or augering) to possibly infer landscape-scale subsurface flow paths. In areas where subsurface interface topography varies similarly with surface topography, surface DEM can be used to simulate potential subsurface lateral flow path reasonably so the cost associated with obtaining depth to subsurface water-restricting layer can be minimized.

Simulated ground-water flow and sources of water in the Killbuck Creek Valley near Wooster, Wayne County, Ohio

USGS Publications Warehouse

Breen, K.J.; Kontis, A.L.; Rowe, G.L.; Haefner, R.J.

1995-01-01

The stratified-drift aquifer in the 3,000-ft (feet)-wide and 100-ft-deep buried valley of Killbuck Creek near Wooster in northeastern Ohio was studied. The stratified drift with adjacent sandstone and shale bedrock produce a system of ground-water flow representative of the western part of the glaciated north-eastern United States. The stratified-drift aquifer is an excellent source of water for municipal and industrial wells. The aquifer is recharged locally by water from precipitation on the valley floor and uplands, by infiltration from streams, and by lateral flow to the valley from the uplands. As a result, the aquifer is vulnerable to surface or subsurface spills of contaminants in the valley or the adjacent uplands. Quality of water in the stratified drift is affected by influx of water from bedrock lateral to or beneath the valley. This influx is controlled, in part, by the pumping stress placed on the stratified-drift aquifer. Hydrogeologic and aqueous-geochemical data were analyzed to establish the framework necessary for stead-state and transient simulations of ground-water flow in stratified drift and bedrock with a three-layer ground-water-flow model. A new model routine, the Variable-Recharge procedure, was developed to simulate areal recharge and the contribution of the uplands to the drift system. This procedure allows for water applied to land surface to infiltrate or to be rejected. Rejected recharge and ground water discharged when the water table is at land surface form surface runoff-this excess upland water can be redirected as runoff to other parts of the model. Infiltration of streamwater, areal recharge to uplands and valley, and lateral subsurface flow from the uplands to the valley are sources of water to the stratufued0druft aquifer. Water is removed from the stratified-drift aquifer at Wooster primarily by production wells pumping at a rate of approximately 8.5 ft3/s (cubic feet per second). The ground-water budget resulting from two types of simulations of ground-water flow in this study indicates the primary sources of water to the wells are recharge at or near land surface and lateral subsurface flow from the shale and sandstone bedrock. Components of recharge at land surface include induced infiltration from streams, precipitation on the valley floor, and infiltration of unchanneled upland runoff that reaches the valley floor. The steady-state simulation was designed to represent conditions during the fall of 1984. The transient simulation was designed to represent an 11-day snowmelt event, 23 February to 5 March 1985, that caused water levels to rise significantly throughout the valley. Areal recharge to the valley and flow from the uplands to the valley were determined through the Variable-Recharge procedure. The total steady-state recharge to the valley was 12.5 ft3/s. Upland sources, areal valley recharge, and induced infiltration from Killnuck Creek accounted for 63, 23, and 8 percent, respectively, of the valley recharge. An analysis of the simulated vertical flow to the buried stratified drift through surficial slit, clay, and fine sand indicates that about 75 percent of the total recharge to the buried deposits is the sum of areally extensive, relatively small flows less than about 0.01 ft? /s per model node), whereas about 25 percent of the recharge results from a really restricted, relatively large flows (greater than about 0.01 ft? /s per model node). The large-magnitude flows are located primarily beneath Clear and Little Killbuck Creeks where seepage provides abundant recharge and the surficial sediments grade into coarser alluvial-fan deposits. Chemical and isotopic studies of ground water and streamwater combined with measurements of stream infiltration provide independent support for the conclusions derived from computer simulation of ground-water flow. In addition, the chemical and isotopic studies helped quantity the rate and pathways of infiltrating water from

Multiphase transport in polymer electrolyte membrane fuel cells

NASA Astrophysics Data System (ADS)

Gauthier, Eric D.

Polymer electrolyte membrane fuel cells (PEMFCs) enable efficient conversion of fuels to electricity. They have enormous potential due to the high energy density of the fuels they utilize (hydrogen or alcohols). Power density is a major limitation to wide-scale introduction of PEMFCs. Power density in hydrogen fuel cells is limited by accumulation of water in what is termed fuel cell `flooding.' Flooding may occur in either the gas diffusion layer (GDL) or within the flow channels of the bipolar plate. These components comprise the electrodes of the fuel cell and balance transport of reactants/products with electrical conductivity. This thesis explores the role of electrode materials in the fuel cell and examines the fundamental connection between material properties and multiphase transport processes. Water is generated at the cathode catalyst layer. As liquid water accumulates it will utilize the largest pores in the GDL to go from the catalyst layer to the flow channels. Water collects to large pores via lateral transport at the interface between the GDL and catalyst layer. We have shown that water may be collected in these large pores from several centimeters away, suggesting that we could engineer the GDL to control flooding with careful placement and distribution of large flow-directing pores. Once liquid water is in the flow channels it forms slugs that block gas flow. The slugs are pushed along the channel by a pressure gradient that is dependent on the material wettability. The permeable nature of the GDL also plays a major role in slug growth and allowing bypass of gas between adjacent channels. Direct methanol fuel cells (DMFCs) have analogous multiphase flow issues where carbon dioxide bubbles accumulate, `blinding' regions of the fuel cell. This problem is fundamentally similar to water management in hydrogen fuel cells but with a gas/liquid phase inversion. Gas bubbles move laterally through the porous GDL and emerge to form large bubbles within the flow channel. We have compared the role of GDL materials in liquid drop and gas bubble formation and movement within fuel cells.

Assembling an ignimbrite: Compositionally defined eruptive packages in the 1912 Valley of Ten Thousand Smokes ignimbrite, Alaska

USGS Publications Warehouse

Fierstein, J.; Wilson, C.J.N.

2005-01-01

The 1912 Valley of Ten Thousand Smokes (VTTS) ignimbrite was constructed from 9 compositionally distinct, sequentially emplaced packages, each with distinct proportions of rhyolite (R), dacite (D), and andesite (A) pumices that permit us to map package boundaries and flow paths from vent to distal extents. Changing pumice proportions and interbedding relationships link ignimbrite formation to coeval fall deposition during the first ???16 h (Episode I) of the eruption. Pumice compositional proportions in the ignimbrite were estimated by counts on ???100 lapilli at multiple levels in vertical sections wherever accessible and more widely over most of the ignimbrite surface in the VTTS. The initial, 100% rhyolite ignimbrite package (equivalent to regional fall Layer A and occupying ???3.5 h) was followed by packages with increasing proportions of andesite, then dacite, emplaced over ???12.5 h and equivalent to regional fall Layers B1-B3. Coeval fall deposits are locally intercalated with the ignimbrite and show parallel changes in R:D (rhyolite:dacite) proportions, but lack significant amounts of andesite. Andesite was thus dominantly a low-fountaining component in the eruption column and is preferentially represented in packages filling the VTTS north of the vent. The most extensive packages (3 and 4) occur in B1 and early B2 times where flow mobility and volume were optimized; earlier all-rhyolite flows (Package 1) were highly energetic but less voluminous, while later packages (5-9) were both less voluminous and emplaced at lower velocities. Package boundaries are expressed as one or more of the following: sharp color changes corresponding to compositional variations; persistent finer-grained basal parts of flow units; compaction swales filled by later packages; erosional channels cut by the flows that fill them; lobate accumulations of one package; and (mostly south of the vent) intercalated fall deposit layers. Clear flow-unit boundaries are best developed between ignimbrite of non-successive packages, indicating time breaks of tens of minutes to hours. Less well-defined stratification may represent rapidly emplaced successive flow units but often changes over short distances and indicates variations in localized depositional conditions. ?? 2005 Geological Society of America.

Balancing aggradation and progradation on a vegetated delta: The importance of fluctuating discharge in depositional systems

DOE PAGES

Piliouras, Anastasia; Kim, Wonsuck; Carlson, Brandee

2017-10-04

Vegetation is an important component of constructional landscapes, as plants enhance deposition and provide organic sediment that can increase aggradation rates to combat land loss. We conducted two sets of laboratory experiments using alfalfa ( Medicago sativa) to determine the effects of plants on channel organization and large-scale delta dynamics. In the first set, we found that rapid vegetation colonization enhanced deposition but inhibited channelization via increased form drag that reduced the shear stress available for sediment entrainment and transport. A second set of experiments used discharge fluctuations between flood and base flow (or interflood). Interfloods were critical for reworkingmore » the topset via channel incision and lateral migration to create channel relief and prevent rapid plant colonization. These low flow periods also greatly reduced the topset slope in the absence of vegetation by removing topset sediment and delivering it to the shoreline. Floods decreased relief by filling channels with sediment, resulting in periods of rapid progradation and enhanced aggradation over the topset surface, which was amplified by vegetation. The combination of discharge fluctuations and vegetation thus provided a balance of vertical aggradation and lateral progradation. We conclude that plants can inhibit channelization in depositional systems, and that discharge fluctuations encourage channel network organization to naturally balance against aggradation. Furthermore, variations in discharge are an important aspect of understanding the ecomorphodynamics of aggrading surfaces and modeling vegetated deltaic systems, and the combined influences of plants and discharge variations can act to balance vertical and lateral delta growth.« less

Balancing aggradation and progradation on a vegetated delta: The importance of fluctuating discharge in depositional systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Piliouras, Anastasia; Kim, Wonsuck; Carlson, Brandee

Vegetation is an important component of constructional landscapes, as plants enhance deposition and provide organic sediment that can increase aggradation rates to combat land loss. We conducted two sets of laboratory experiments using alfalfa ( Medicago sativa) to determine the effects of plants on channel organization and large-scale delta dynamics. In the first set, we found that rapid vegetation colonization enhanced deposition but inhibited channelization via increased form drag that reduced the shear stress available for sediment entrainment and transport. A second set of experiments used discharge fluctuations between flood and base flow (or interflood). Interfloods were critical for reworkingmore » the topset via channel incision and lateral migration to create channel relief and prevent rapid plant colonization. These low flow periods also greatly reduced the topset slope in the absence of vegetation by removing topset sediment and delivering it to the shoreline. Floods decreased relief by filling channels with sediment, resulting in periods of rapid progradation and enhanced aggradation over the topset surface, which was amplified by vegetation. The combination of discharge fluctuations and vegetation thus provided a balance of vertical aggradation and lateral progradation. We conclude that plants can inhibit channelization in depositional systems, and that discharge fluctuations encourage channel network organization to naturally balance against aggradation. Furthermore, variations in discharge are an important aspect of understanding the ecomorphodynamics of aggrading surfaces and modeling vegetated deltaic systems, and the combined influences of plants and discharge variations can act to balance vertical and lateral delta growth.« less

A water-budget approach to restoring a sedge fen affected by diking and ditching

USGS Publications Warehouse

Wilcox, Douglas A.; Sweat, Michael J.; Carlson, Martha L.; Kowalski, Kurt P.

2006-01-01

A vast, ground-water-supported sedge fen in the Upper Peninsula of Michigan, USA was ditched in the early 1900s in a failed attempt to promote agriculture. Dikes were later constructed to impound seasonal sheet surface flows for waterfowl management. The US Fish and Wildlife Service, which now manages the wetland as part of Seney National Wildlife Refuge, sought to redirect water flows from impounded C-3 Pool to reduce erosion in downstream Walsh Ditch, reduce ground-water losses into the ditch, and restore sheet flows of surface water to the peatland. A water budget was developed for C-3 Pool, which serves as the central receiving and distribution body for water in the affected wetland. Surface-water inflows and outflows were measured in associated ditches and natural creeks, ground-water flows were estimated using a network of wells and piezometers, and precipitation and evaporation/evapotranspiration components were estimated using local meteorological data. Water budgets for the 1999 springtime peak flow period and the 1999 water year were used to estimate required releases of water from C-3 Pool via outlets other than Walsh Ditch and to guide other restoration activities. Refuge managers subsequently used these results to guide restoration efforts, including construction of earthen dams in Walsh Ditch upslope from the pool to stop surface flow, installation of new water-control structures to redirect surface water to sheet flow and natural creek channels, planning seasonal releases from C-3 Pool to avoid erosion in natural channels, stopping flow in downslope Walsh Ditch to reduce erosion, and using constructed earthen dams and natural beaver dams to flood the ditch channel below C-3 Pool. Interactions between ground water and surface water are critical for maintaining ecosystem processes in many wetlands, and management actions directed at restoring either ground- or surface-water flow patterns often affect both of these components of the water budget. This approach could thus prove useful in guiding restoration efforts in many hydrologically altered and managed wetlands worldwide.

Seasonal Variation of Submesoscale Flow Features in a Mesoscale Eddy-dominant Region in the East Sea

NASA Astrophysics Data System (ADS)

Chang, Yeon S.; Choi, Byoung-Ju; Park, Young-Gyu

2018-03-01

Seasonal changes in the distribution of submesoscale (SM) flow features were examined using a fine-resolution numerical simulation. The SM flows are expected to be strong where mesoscale (MS) eddies actively develop and also when the mixed layer depth (MLD) is deep due to enhanced baroclinic instability. In the East Sea (ES), MS eddies more actively develop in summer while the MLD is deeper in winter, which provided the motivation to conduct this study to test the effects of MLD and MS eddies on the SM activity in this region. Finite-scale Liapunov exponents and the vertical velocity components were employed to analyze the SM activities. It was found that the SM intensity was marked by seasonality: it is stronger in winter when the mixed layer is deep but weaker in summer - despite the greater eddy kinetic energy. This is because in summer the mixed layer is so thin that there is not enough available potential energy. When the SM activity was quantified based on parameterization, (MLD × density gradient), it was determined that the seasonal variation of MLD plays a more important role than the lateral density gradient variation on SM flow motion in the ES.

Simulating Heterogeneous Infiltration and Contaminant leaching Processes at Chalk River, Ontario

NASA Astrophysics Data System (ADS)

Ali, M. A.; Ireson, A. M.; Keim, D.

2015-12-01

A study is conducted at a waste management area in Chalk River, Ontario to characterize flow and contaminant transport with the aim of contributing to improved hydrogeological risk assessment in the context of waste management. Field monitoring has been performed to gain insights into the unsaturated zone characteristics, moisture dynamics, and contaminant transport rates. The objective is to provide quantitative estimates of surface fluxes (quantification of infiltration and evaporation) and investigations of unsaturated zone processes controlling water infiltration and spatial variability in head distributions and flow rates. One particular issue is to examine the effectiveness of the clayey soil cap installed to prevent infiltration of water into the waste repository and the top sand soil cover above the clayey layer to divert the infiltrated water laterally. The spatial variability in the unsaturated zone properties and associated effects on water flow and contaminant transport observed at the site, have led to a concerted effort to develop improved model of flow and transport based on stochastic concepts. Results obtained through the unsaturated zone model investigations are combined with the hydrogeological and geochemical components and develop predictive tools to assess the long term fate of the contaminants at the waste management site.

Sedimentation problems in a lateral dock on the Paraná River

NASA Astrophysics Data System (ADS)

Latessa, Gaston; Sabarots Gerbec, Martin; Arecco, Pablo

2017-04-01

The Paraná River is one of the largest water courses in the world and along its reach in the Argentine territory, it receives a large load of sediments from the Pilcomayo and Bermejo Rivers, through the Paraguay River, in the upper basin at the North of Argentina and South of Bolivia. The suspended sediment load is estimated in 100 Million ton/year. This unique characteristic drives the Paraná River morphology downstream, as well as the Paraná delta morphodynamics. On top of its natural behaviour, the Paraná-Paraguay river system is an important inland waterway transport corridor, with a significant amount of sea going vessels and inland barges navigating throughout stretches of more than 3000 Km. Consequently, there are numerous port complexes and terminals along the river banks. The typical wet infrastructure of these terminals is usually composed by jetties and quay walls, and occasionally with side or lateral docks. Whereas, the case included within this study presents all these components. This study presents a hydrodynamic and sedimentology 3D model to predict the velocity fields and the associated shear stresses that will drive morphological processes in the lateral dock. The terminal layout, side dock configuration, and sedimentation issues will be analyzed from multidisciplinary point of view, under different hydrological events and considering the correlated sediment loads. Recent bathymetry studies had been carried out and this set of data will be implemented to build the domain geometry. The flow series is as well extended with the up to date gauged flows and levels, to carry out statistical analysis and identify the design flows for different probabilities. The main objective of this analysis will be to understand and identify the scour and deposition processes and the possible problems to the structures safety and the operation of the docks, and introduce variations to the baseline design, if necessary. Results will be contrasted and validated with empirical formulae and criteria.

Does the Alpha-defensin Immunoassay or the Lateral Flow Test Have Better Diagnostic Value for Periprosthetic Joint Infection? A Systematic Review.

PubMed

Eriksson, Hannah K; Nordström, Jakob; Gabrysch, Katja; Hailer, Nils P; Lazarinis, Stergios

2018-05-01

Measuring alpha-defensin concentrations in synovial fluid may help to diagnose periprosthetic joint infection (PJI). There are two commercially available methods for measuring alpha-defensin in synovial fluid: the enzyme-linked immunosorbent assay-based Synovasure® alpha-defensin immunoassay, which gives a numeric readout within 24 hours, and the Synovasure lateral flow test, which gives a binary readout within 20 minutes. There is no compilation of the existing literature to support the use of one of these two tests over the other. Does the immunoassay or the lateral flow test have better diagnostic value (sensitivity and specificity) in diagnosing PJI? We followed PRISMA guidelines and identified all studies on alpha-defensin concentration in synovial fluid as a PJI diagnostic marker, indexed to April 14, 2017, in PubMed, JSTOR, Google Scholar, and OVID databases. The search retrieved 1578 records. All prospective and retrospective studies on alpha-defensin as a PJI marker (PJI classified according to the criteria of the Musculoskeletal Infection Society) after THA or TKA were included in the analysis. All studies used only one of the two commercially available test methods, but none of them was comparative. After excluding studies with overlapping patient populations, four studies investigating the alpha-defensin immunoassay and three investigating the lateral flow test remained. Alpha-defensin immunoassay studies included 482 joints and lateral flow test studies included 119. The quality of the trials was assessed according to the Quality Assessment of Diagnostic Accuracy Studies (QUADAS-2) tool. The heterogeneity among studies was evaluated by the I index, indicating that the heterogeneity of the included studies was low. Pooled sensitivity, specificity, positive and negative likelihood ratios, and receiver operating curves were calculated for each method and compared with each other. The alpha-defensin immunoassay had superior overall diagnostic value compared with the lateral flow test (area under the curve, 0.98 versus 0.75) with higher sensitivity (96% [90%-98%] versus 71% [55%-83%], p < 0.001), but no difference in specificity with the numbers available (96% [93%-97%] versus 90% [81%-95%], p = 0.060). Measurement of alpha-defensin in synovial fluid is a valuable complement to existing diagnostic criteria, and the immunoassay test detects PJI more accurately than the lateral flow test. The lateral flow test has lower sensitivity, making it difficult to rule out infection, but its relatively high specificity combined with the advantage of a quick response time can make it useful to rule in infection perioperatively. Level III, diagnostic study.

Assessment of the hydrogeology and water quality in a near-shore well field, Sarasota, Florida

USGS Publications Warehouse

Broska, J.C.; Knochenmus, L.A.

1996-01-01

The city of Sarasota, Florida, operates a downtown well field that pumps mineralized water from ground water sources to supply a reverse osmosis plant. Because of the close proximity of the well field to Sarasota Bay and the high sulfate and chloride concentrations of ground-water supplies, a growing concern exists about the possibility of lateral movement of saltwater in a landward direction (intrusion) and vertical movement of relict sea water (upconing). In 1992, the U.S. Geological Survey began a 3-year study to evaluate the hydraulic characteristics and water quality of ground-water resources within the downtown well field and the surrounding 235-square-mile study area. Delineation of the hydrogeology of the study area was based on water- quality data, aquifer test data, and extensive borehole geophysical surveys (including gamma, caliper, temperature, electrical resistivity, and flow meter logs) from the six existing production wells and from a corehole drilled as part of the study, as well as from published and unpublished reports on file at the U.S. Geological Survey, the Southwest Florida Water Management District, and consultant's reports. Water-quality data were examined for spatial and temporal trends that might relate to the mechanism for observed water-quality changes. Water quality in the study area appears to be dependent upon several mechanisms, including upconing of higher salinity water from deeper zones within the aquifer system, interbore-hole flow between zones of varying water quality through improperly cased and corroded wells, migration of highly mineralized waters through structural deformities, and the presence of unflushed relict seawater. A numerical ground-water flow model was developed as an interpretative tool where field-derived hydrologic characteristics could be tested. The conceptual model consisted of seven layers to represent the multilayered aquifer systems underlying the study area. Particle tracking was utilized to delineate the travel path of water as it enters the model area under a set of given conditions. Within the model area, simulated flow in the intermediate aquifer system originates primarily from the northwestern boundary. Simulated flow in the Upper Floridan aquifer originates in lower model layers (deeper flow zones) and ultimately can be traced to the southeastern and northwestern boundaries. Volumetric budgets calculated from numerical simulation of a hypothetical well field indicate that the area of contribution to the well field changes seasonally. Although ground-water flow patterns change with wet and dry seasons, most water enters the well-field flow system through lower parts of the Upper Floridan aquifer from a southeastern direction. Moreover, particle tracking indicated that ground-water flow paths with strictly lateral pathlines in model layers correspond to the intermediate aquifer system, whereas particles traced through model layers corresponding to the Upper Floridan aquifer had components of vertical and lateral flow.

Sedentary behavior as a factor in determining lateral line contributions to rheotaxis.

PubMed

Bak-Coleman, Joseph; Coombs, Sheryl

2014-07-01

Rheotaxis is a robust, multisensory behavior with many potential benefits for fish and other aquatic animals. Visual (optic flow) cues appear to be sufficient for rheotaxis, but other sensory cues can clearly compensate for the loss of vision. The role of various non-visual sensory systems, in particular the flow-sensing lateral line, is poorly understood, largely because of widely varying methods and sensory conditions for studying rheotaxis. Here, we examine how sedentary behavior under visually deprived conditions affects the relative importance of lateral line cues in two species: one that is normally sedentary (the three-lined corydoras, Corydoras trilineatus) and one that normally swims continuously along the substrate (the blind cavefish, Astyanax mexicanus). No effect of lateral line disruption on rheotactic performance was found in blind cavefish, which were significantly more mobile than three-lined corydoras. By contrast, rheotaxis was significantly impaired at low, but not high, flow speeds in lateral-line-disabled corydoras. In addition, lateral-line-enabled corydoras were characterized by decreased mobility and increased rheotactic performance relative to lateral-line-disabled fish. Taken together, these results suggest that sedentary behavior is an important factor in promoting reliance on lateral line cues. © 2014. Published by The Company of Biologists Ltd.

Impingement of the Mobile Bearing on the Lateral Wall of the Tibial Tray in Unicompartmental Knee Arthroplasty.

PubMed

Inui, Hiroshi; Taketomi, Shuji; Yamagami, Ryota; Sanada, Takaki; Shirakawa, Nobuyuki; Tanaka, Sakae

2016-07-01

Tilting of the mobile bearing relative to the tibial tray in the flexion position may result from the implantation of femoral components more laterally relative to tibial components during unicompartmental knee arthroplasty (UKA) using the Oxford Knee. The purpose of the present study was to compare femoral component positions after UKA using the phase 3 device and a novel device. We further evaluated the placement of the femoral components with the new device in the flexion position to determine the association with short-term prognosis. The location of femoral and tibial components in the flexion position of 38 knees implanted using the phase 3 device and 49 knees using a novel device was assessed at 1 year postoperatively using radiography of the proximal tibia and distal femur in the flexion position. The femoral component was implanted more laterally using the new device than using the phase 3 device in the flexion position (P = .012), which caused the impingement of the mobile bearing against the lateral wall of the tibial tray. After UKA using the new device, 10% of patients exhibited the tilting phenomenon of the mobile bearing because of the lateral implantation of the femoral implant. To prevent implantation of the femoral component too laterally using the new device during UKA, knee surgeons should set the drill guide more medially such that the center of the drill is aligned with the middle of the medial femoral condyle. Copyright © 2016 Elsevier Inc. All rights reserved.

Nonperiodic eddy pulsations

USGS Publications Warehouse

Rubin, David M.; McDonald, Richard R.

1995-01-01

Recirculating flow in lateral separation eddies is typically weaker than main stem flow and provides an effective environment for trapping sediment. Observations of recirculating flow and sedimentary structures demonstrate that eddies pulsate in size and in flow velocity even when main stem flow is steady. Time series measurements of flow velocity and location of the reattachment point indicate that these pulsations are nonperiodic. Nonperiodic flow in the lee of a channel margin constriction is grossly different from the periodic flow in the lee of a cylinder that is isolated in a flow. Our experiments demonstrate that placing a flow-parallel plate adjacent to a cylinder is sufficient to cause the leeside flow to change from a periodic sequence of vortices to a nonperiodically pulsating lateral separation eddy, even if flow conditions are otherwise unchanged. Two processes cause the leeside flow to become nonperiodic when the plate is added. First, vortices that are shed from the cylinder deform and become irregular as they impact the plate or interfere with remnants of other vortices near the reattachment point. Second, these deformed vortices and other flow structures are recirculated in the lateral separation eddy, thereby influencing the future state (pressure and momentum distribution) of the recirculating flow. The vortex deformation process was confirmed experimentally by documenting spatial differences in leeside flow; vortex shedding that is evident near the separation point is undetectable near the reattachment point. Nonlinear forecasting techniques were used in an attempt to distinguish among several possible kinds of nonperiodic flows. The computational techniques were unable to demonstrate that any of the nonperiodic flows result from low-dimensional nonlinear processes.

Lateral flow immunoassay for the rapid detection of citrus tristeza virus

USDA-ARS?s Scientific Manuscript database

A lateral flow methodology was developed using gold nanoparticles for rapid detection of Citrus tristeza virus (CTV). The test strip was based on a sandwich immunoassay and could be accomplished within 10 minutes. A sample was considered negative for CTV when only the control line appeared; whereas,...

Measuring acetabular component position on lateral radiographs - ischio-lateral method.

PubMed

Pulos, Nicholas; Tiberi Iii, John V; Schmalzried, Thomas P

2011-01-01

The standard method for the evaluation of arthritis and postoperative assessment of arthroplasty treatment is observation and measurement from plain films, using the flm edge for orientation. A more recent employment of an anatomical landmark, the ischial tuberosity, has come into use as orientation for evaluation and is called the ischio-lateral method. In this study, the use of this method was evaluated as a first report to the literature on acetabular component measurement using a skeletal reference with lateral radiographs. Postoperative radiographs of 52 hips, with at least three true lateral radiographs taken at different time periods, were analyzed. Component position was measured with the historical method (using the flm edge for orientation) and with the new method using the ischio-lateral method. The mean standard deviation (SD) for the historical approach was 3.7° and for the ischio-lateral method, 2.2° (p < 0.001). With the historical method, 19 (36.5%) hips had a SD greater than ± 4°, compared to six hips (11.5%) with the ischio-lateral method. By using a skeletal reference, the ischio-lateral method provides a more consistent measurement of acetabular component position. The high intra-class correlation coefficients for both intra- and inter-observer reliability indicate that the angle measured with this simple method, which employs no further technology, increased time, or cost, is consistent and reproducible for multiple observers.

Why is there evidence for flowing ice at mid-latitudes on Mars but not at the poles?

NASA Astrophysics Data System (ADS)

Smith, I. B.

2017-12-01

Ice has been detected on Mars in many places, from the polar caps, to mid-latitudes. In many locations there exists evidence for glacial flow. This raises the possibility of flow for the polar layered deposits (PLD). Since the >2000 m thick ice deposits were first observed, speculation about their flow status have persisted. Several stratigraphic predictions regarding flow have been made (Figure 1), but these predictions are not supported with observational data (Smith and Holt 2015) The disagreement between model and observations has led to a general consensus that the polar ice flows more slowly than other processes acting on the PLD, but the reasoning is not understood. Here I posit that the polar layered deposits do not act as a single, generic ice sheet. Instead, they act as a stack of thin ice sheets, where each layer is separated by a boundary of dust, and all layers flow individually. The layers act as barriers to vertical flow, so the viscosity of the cold ice can only be expressed through lateral expansion. I plan to present a simple experiment demonstrating the multi-layer, stacked flow hypothesis. I will demonstrate that the layers themselves flow but do not deform the entire ice sheet, as previously predicted. This allows for the PLD to retain their steep slopes and prevents many of the predicted flow features to form. The major component of this hypothesis is that the dust layers hinder flow. Thus, constraining the friction coefficient, viscosity, tensile strength and compressibility of the dust layers becomes an important next step for testing the stacked, multi-layer flow scenario. Acknowledgements: Thanks to Eric Larour and David Goldsby for helpful comments.

Experimental investigation of lateral forces induced by flow through model labyrinth glands

NASA Technical Reports Server (NTRS)

Leong, Y. M. M. S.; Brown, R. D.

1984-01-01

The lateral forces induced by flow through model labyrinth glands were investigated. Circumferential pressure distributions, lateral forces and stiffness coefficients data obtained are discussed. The force system is represented as a negative spring and a tangential force orthogonal to eccentricity. The magnitude of these forces are dependent on eccentricity, entry swirl, rotor peripheral velocity and seal size. A pressure equalization chamber at midgland tests should in significantly reduced forces and stiffness coefficients.

Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein.

PubMed

Sinawang, Prima Dewi; Rai, Varun; Ionescu, Rodica E; Marks, Robert S

2016-03-15

An Electrochemical Lateral Flow Immunosensor (ELFI) is developed combining screen-printed gold electrodes (SPGE) enabling quantification together with the convenience of a lateral flow test strip. A cellulose glassy fiber paper conjugate pad retains the marker immunoelectroactive nanobeads which will bind to the target analyte of interest. The specific immunorecognition event continues to occur along the lateral flow bed until reaching the SPGE-capture antibodies at the end of the cellulosic lateral flow strip. The rationale of the immunoassay consists in the analyte antigen NS1 protein being captured selectively and specifically by the dengue NS1 antibody conjugated onto the immunonanobeads thus forming an immunocomplex. With the aid of a running buffer, the immunocomplexes flow and reach the immuno-conjugated electrode surface and form specific sandwich-type detection due to specific, molecular recognition, while unbound beads move along past the electrodes. The successful sandwich immunocomplex formation is then recorded electrochemically. Specific detection of NS1 is translated into an electrochemical signal contributed by a redox label present on the bead-immobilized detection dengue NS1 antibody while a proportional increase of faradic current is observed with increase in analyte NS1 protein concentration. The first generation ELFI prototype is simply assembled in a cassette and successfully demonstrates wide linear range over a concentration range of 1-25 ng/mL with an ultrasensitive detection limit of 0.5 ng/mL for the qualitative and quantitative detection of analyte dengue NS1 protein. Copyright © 2015 Elsevier B.V. All rights reserved.

Transient Droplet Behavior and Droplet Breakup during Bulk and Confined Shear Flow in Blends with One Viscoelastic Component: Experiments, Modelling and Simulations

DOE Office of Scientific and Technical Information (OSTI.GOV)

Cardinaels, Ruth; Verhulst, Kristof; Moldenaers, Paula

2008-07-07

The transient droplet deformation and droplet orientation after inception of shear, the shape relaxation after cessation of shear and droplet breakup during shear, are microscopically studied, both under bulk and confined conditions. The studied blends contain one viscoelastic Boger fluid phase. A counter rotating setup, based on a Paar Physica MCR300, is used for the droplet visualisation. For bulk shear flow, it is shown that the droplet deformation during startup of shear flow and the shape relaxation after cessation of shear flow are hardly influenced by droplet viscoelasticity, even at moderate to high capillary and Deborah numbers. The effects ofmore » droplet viscoelasticity only become visible close to the critical conditions and a novel break-up mechanism is observed. Matrix viscoelasticity has a more pronounced effect, causing overshoots in the deformation and significantly inhibiting relaxation. However, different applied capillary numbers prior to cessation of shear flow, with the Deborah number fixed, still result in a single master curve for shape retraction, as in fully Newtonian systems. The long tail in the droplet relaxation can be qualitatively described with a phenomenological model for droplet deformation, when using a 5-mode Giesekus model for the fluid rheology. It is found that the shear flow history significantly affects the droplet shape evolution and the breakup process in blends with one viscoelastic component. Confining a droplet between two plates accelerates the droplet deformation kinetics, similar to fully Newtonian systems. However, the increased droplet deformation, due to wall effects, causes the steady state to be reached at a later instant in time. Droplet relaxation is less sensitive to confinement, leading to slower relaxation kinetics only for highly confined droplets. For the blend with a viscoelastic droplet, a non-monotonous trend is found for the critical capillary number as a function of the confinement ratio. Finally, experimental data are compared with 3D simulations, performed with a volume-of-fluid algorithm.« less

Investigation of drag and heat reduction induced by a novel combinational lateral jet and spike concept in supersonic flows based on conjugate heat transfer approach

NASA Astrophysics Data System (ADS)

Zhu, Liang; Chen, Xiong; Li, Yingkun; Musa, Omer; Zhou, Changsheng

2018-01-01

When flying at supersonic or hypersonic speeds through the air, the drag and severe heating have a great impact on the vehicles, thus the drag reduction and thermal protection studies have attracted worldwide attention. In the current study, the Reynolds-averaged Navier-Stokes (RANS) equations coupled with the shear stress transport (SST) k - ω turbulence model have been employed to investigate the flow behavior induced by a novel combinational lateral jet and spike concept in supersonic flows. A coupling conjugate heat transfer (CHT) approach has been applied to investigate the thermal protection, which takes the heat transfer of structure into consideration. After the code was validated by the available experimental results and the gird independency analysis was carried out, the influences of the spike length ratio, lateral jet pressure ratio and lateral jet location on the drag and heat reduction performance are analyzed comprehensively. The obtained results show that a remarkable reduction in the drag and heat flux is achieved when a lateral jet is added to the spike. This implies that the combinational lateral jet and spike concept in supersonic flows have a great benefit to the drag and heat reduction. Both the drag and heat reduction decrease with the increase of the lateral jet pressure ratio, and the heat flux is more sensitive to the lateral jet pressure ratio. The lateral jet should not be located in the bottom of the spike in order to realize better drag and heat reduction performance. The drag and heat flux could be reduced by about 45% by reasonable lateral jet location. The drag decreases with the increase of the spike length ratio whereas the heat flux is affected by the spike length ratio just in a certain range.

Lateral and vertical facies relationships of bedforms deposited by aggrading supercritical flows: From cyclic steps to humpback dunes

NASA Astrophysics Data System (ADS)

Lang, Jörg; Winsemann, Jutta

2013-10-01

The preservation of bedforms related to supercritical flows and hydraulic jumps is commonly considered to be rare in the geologic record, although these bedforms are known from a variety of depositional environments. This field-based study presents a detailed analysis of the sedimentary facies and stacking pattern of deposits of cyclic steps, chutes-and-pools, antidunes and humpback dunes from three-dimensional outcrops. The well exposed Middle Pleistocene successions from northern Germany comprise glacilacustrine ice-contact subaqueous fan and glacial lake-outburst flood deposits. The studied successions give new insights into the depositional architecture of bedforms related to supercritical flows and may serve as an analogue for other high-energy depositional environments such as fluvial settings, coarse-grained deltas or turbidite systems. Deposits of cyclic steps occur within the glacial lake-outburst flood succession and are characterised by lenticular scours infilled by gently to steeply dipping backsets. Cyclic steps formed due to acceleration and flow thinning when the glacial lake-outburst flood spilled over a push-moraine ridge. These bedforms are commonly laterally and vertically truncated and alternate with deposits of chutes-and-pools and antidunes. The subaqueous fan successions are dominated by laterally extensive sinusoidal waveforms, which are interpreted as deposits of aggrading stationary antidunes, which require quasi-steady flows at the lower limit of the supercritical flow stage and high rates of sedimentation. Humpback dunes are characterised by downflow divergent cross-stratification, displaying differentiation into topsets, foresets and bottomsets, and are interpreted as deposited at the transition from subcritical to supercritical flow conditions or vice versa. Gradual lateral and vertical transitions between humpback dunes and antidune deposits are very common. The absence of planar-parallel stratification in all studied successions suggests that the formation of these bedforms is suppressed in flows characterised by hydraulic jumps under highly aggradational conditions. The large-scale lateral and vertical successions of bedforms are interpreted as representing the temporal and spatial evolution of the initial supercritical flows, which was strongly affected by the occurrence of hydraulic jumps. Small-scale facies changes and the formation of individual bedforms are interpreted as controlled by fluctuating discharge, bed topography and pulsating unstable flows.

Ultrasonic vibration double scratch morphology and scratching force of BK7 glass

NASA Astrophysics Data System (ADS)

Wang, Chu; Wang, Hongxiang; Liu, Junliang; Gao, Shi

2018-03-01

In this paper, the damage morphology and scratching force of BK7 glass components were analyzed by ultrasonic vibration double-scratch test. The results showed that there was surface damage caused by plastic flow and brittle fracture during the scratching process, and the scratching depth and the distance between the two scratches had effect on the propagation and overlapping of lateral cracks. When the scratching depth was small, the jagged scratch was produced on the surface, and accompanied by a small amount of tiny pieces of debris off. With the increase in scratching depth, the lateral cracks caused by scratching overlapped and expended to form a mesh sheet, and then fell off from the surface. When the scratching distance was small, the interaction of the cracks caused large slice of material to fall off. With the increase in scratching distance, the area between the two scratches was not easy to occur the overlapping of the lateral cracks. In addition, with the increase of the scratching depth, the scratching force showed a gradual increase trend, and the scratching force of the second scratch would increase with the scratching distance.

Channeling at the base of the lithosphere during the lateral flow of plume material beneath flow line hot spots

NASA Astrophysics Data System (ADS)

Sleep, Norman H.

2008-08-01

Chains of volcanic edifices lie along flow lines between plume-fed hot spots and the thin lithosphere at ridge axes. Discovery and Euterpe/Musicians Seamounts are two examples. An attractive hypothesis is that buoyant plume material flows along the base of the lithosphere perpendicular to isochrons. The plume material may conceivably flow in a broad front or flow within channels convectively eroded into the base to the lithosphere. A necessary but not sufficient condition for convective channeling is that the expected stagnant-lid heat flow for the maximum temperature of the plume material is comparable to the half-space surface heat flow of the oceanic lithosphere. Two-dimensional and three-dimensional numerical calculations confirm this inference. A second criterion for significant convective erosion is that it needs to occur before the plume material thins by lateral spreading. Scaling relationships indicate spreading and convection are closely related. Mathematically, the Nusselt number (ratio of convective to conductive heat flow in the plume material) scales with the flux (volume per time per length of flow front) of the plume material. A blob of unconfined plume material thus spreads before the lithosphere thins much and evolves to a slowly spreading and slowly convecting warm region in equilibrium with conduction into the base of the overlying lithosphere. Three-dimensional calculations illustrate this long-lasting (and hence observable) state of plume material away from its plume source. A different flow domain occurs around a stationary hot plume that continuously supplies hot material. The plume convectively erodes the overlying lithosphere, trapping the plume material near its orifice. The region of lithosphere underlain by plume material grows toward the ridge axis and laterally by convective thinning of the lithosphere at its edges. The hottest plume material channels along flow lines. Geologically, the regions of lithosphere underlain by either warm or hot plume material are likely to extend laterally away from the volcanic edifices whether or not channeling occurs.

Identification of runoff formation with two dyes in a mid-latitude mountain headwater

NASA Astrophysics Data System (ADS)

Vlcek, Lukas; Schneider, Philipp; Falatkova, Kristyna

2017-04-01

There have been numerous studies on subsurface flow in peat bog areas, as both water scarcity and floods have led to increased attention to this specific environment and its role within the hydrological cycle. In contrast, this experimental study identifies runoff formation at two opposite hillslopes in a peaty mountain headwater; a slope with organic soils (Peat / Histosol) and shallow groundwater ( 0.5 m below surface) complemented by a slope with mineral soils (Podzol) and no detectable groundwater within 2 m below surface. Differences in infiltration, percolation, and preferential flowpaths between both hillslopes could be identified by sprinkling experiments with two dyes - Brilliant Blue FCF and Fluorescein. By excavating dye-stained soil profiles parallel ("lateral") and perpendicular ("frontal") to the slopes' gradients - both within and downstream of the sprinkling plots - dye stained flow patterns in the soil could be clearly identified. The results show that biomat flow occurred at both hillslopes. The dye solutions infiltrated into the soil and continued either as lateral subsurface pipeflow (SSF), in the case of the Peat Bog, or percolated vertically towards the bedrock in the case of the Podzol. The study provides evidence that biomat flow (BMF) - shallow, lateral preferential flowpaths along decomposed tree roots or logs - is a major runoff formation process at the Peat Bog hillslope and in the adjacent riparian zone. This lateral flow through the organic soil hillslope (Peat Bog) towards the stream occurred mainly as shallow subsurface flow in organic layers above the groundwater level (BMF and SSF), but water partly percolates to the shallow groundwater via vertical macropores as well . In contrast, the mineral soil hillslope (Podzol) was mostly dominated by vertical percolation. Lateral flow occurred only on short distances in the organic topsoil as biomat flow (BMF). The sorptive tracer Brilliant Blue FCF successfully stained flowpaths in the soil at both hillslopes, whereas the identification of soil staining patterns by the relatively conservative tracer Fluorescein was limited on organic soil profiles.

A Gradually Varied Approach to Model Turbidity Currents in Submarine Channels

NASA Astrophysics Data System (ADS)

Bolla Pittaluga, M.; Frascati, A.; Falivene, O.

2018-01-01

We develop a one-dimensional model to describe the dynamics of turbidity current flowing in submarine channels. We consider the flow as a steady state polydisperse suspension accounting for water detrainment from the clear water-turbid interface, for spatial variations of the channel width and for water and sediment lateral overspill from the channel levees. Moreover, we account for sediment exchange with the bed extending the model to deal with situations where the current meets a nonerodible bed. Results show that when water detrainment is accounted for, the flow thickness becomes approximately constant proceeding downstream. Similarly, in the presence of channel levees, the flow tends to adjust to channel relief through the lateral loss of water and sediment. As more mud is spilled above the levees relative to sand, the flow becomes more sand rich proceeding downstream when lateral overspill is present. Velocity and flow thickness predicted by the model are then validated by showing good agreement with laboratory observations. Finally, the model is applied to the Monterey Canyon bathymetric data matching satisfactorily the December 2002 event field measurements and predicting a runout length consistent with observations.

Novel development of a lateral flow immunoassay for rapid field detection of citrus tristeza virus

USDA-ARS?s Scientific Manuscript database

Maintenance of virus-free citrus in nurseries and orchards is essential to control spread of aphid-borne Citrus tristeza virus (CTV) in California. A lateral flow assay (LFA) test strip with a polyclonal antiserum made from virus particles produced in Nicotiana benthamiana plants inoculated with an ...

Modifying WEPP to improve streamflow simulation in a Pacific Northwest watershed

Treesearch

A. Srivastava; M. Dobre; J. Q. Wu; W. J. Elliot; E. A. Bruner; S. Dun; E. S. Brooks; I. S. Miller

2013-01-01

The assessment of water yield from hillslopes into streams is critical in managing water supply and aquatic habitat. Streamflow is typically composed of surface runoff, subsurface lateral flow, and groundwater baseflow; baseflow sustains the stream during the dry season. The Water Erosion Prediction Project (WEPP) model simulates surface runoff, subsurface lateral flow...

Automated Low-Cost Smartphone-Based Lateral Flow Saliva Test Reader for Drugs-of-Abuse Detection

PubMed Central

Carrio, Adrian; Sampedro, Carlos; Sanchez-Lopez, Jose Luis; Pimienta, Miguel; Campoy, Pascual

2015-01-01

Lateral flow assay tests are nowadays becoming powerful, low-cost diagnostic tools. Obtaining a result is usually subject to visual interpretation of colored areas on the test by a human operator, introducing subjectivity and the possibility of errors in the extraction of the results. While automated test readers providing a result-consistent solution are widely available, they usually lack portability. In this paper, we present a smartphone-based automated reader for drug-of-abuse lateral flow assay tests, consisting of an inexpensive light box and a smartphone device. Test images captured with the smartphone camera are processed in the device using computer vision and machine learning techniques to perform automatic extraction of the results. A deep validation of the system has been carried out showing the high accuracy of the system. The proposed approach, applicable to any line-based or color-based lateral flow test in the market, effectively reduces the manufacturing costs of the reader and makes it portable and massively available while providing accurate, reliable results. PMID:26610513

An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A

NASA Astrophysics Data System (ADS)

Liu, Jing; Gao, Shan; Kang, Lin; Ji, Bin; Xin, Wenwen; Kang, Jingjing; Li, Ping; Gao, Jie; Wang, Hanbin; Wang, Jinglin; Yang, Hao

2017-03-01

Botulism is a severe and potentially lethal paralytic disease caused by several botulinum neurotoxin-producing Clostridia spp. In China, the majority of the cases caused by botulism were from less-developed rural areas. Here, we designed specific substrate peptides and reconfigured gold nanoparticle-based lateral flow test strip (LFTS) to develop an endopeptidase-based lateral flow assay for the diagnosis of botulism. We performed this lateral flow assay on botulinum neurotoxin-spiked human serum samples. The as-prepared LFTS had excellent performance in the detection of botulinum neurotoxin using only 1 μL of simulated serum, and its sensitivity and specificity were comparable to that of mouse lethality assay. Moreover, the assay takes only half a day and does not require highly trained laboratory staff, specialized facility, or equipment. Finally, our LFTS can be potentially extended to other serotypes of BoNTs by designing specific substrate peptides against the different types of BoNTs. Overall, we demonstrate a strategy by which LFTS and endopeptidase activity assays can be integrated to achieve facile and economic diagnosis of botulism in resource-limited settings.

Assessing hemispheric specialization for processing arithmetic skills in adults: A functional transcranial doppler ultrasonography (fTCD) study.

PubMed

Connaughton, Veronica M; Amiruddin, Azhani; Clunies-Ross, Karen L; French, Noel; Fox, Allison M

2017-05-01

A major model of the cerebral circuits that underpin arithmetic calculation is the triple-code model of numerical processing. This model proposes that the lateralization of mathematical operations is organized across three circuits: a left-hemispheric dominant verbal code; a bilateral magnitude representation of numbers and a bilateral Arabic number code. This study simultaneously measured the blood flow of both middle cerebral arteries using functional transcranial Doppler ultrasonography to assess hemispheric specialization during the performance of both language and arithmetic tasks. The propositions of the triple-code model were assessed in a non-clinical adult group by measuring cerebral blood flow during the performance of multiplication and subtraction problems. Participants were 17 adults aged between 18-27 years. We obtained laterality indices for each type of mathematical operation and compared these in participants with left-hemispheric language dominance. It was hypothesized that blood flow would lateralize to the left hemisphere during the performance of multiplication operations, but would not lateralize during the performance of subtraction operations. Hemispheric blood flow was significantly left lateralized during the multiplication task, but was not lateralized during the subtraction task. Compared to high spatial resolution neuroimaging techniques previously used to measure cerebral lateralization, functional transcranial Doppler ultrasonography is a cost-effective measure that provides a superior temporal representation of arithmetic cognition. These results provide support for the triple-code model of arithmetic processing and offer complementary evidence that multiplication operations are processed differently in the adult brain compared to subtraction operations. Copyright © 2017 Elsevier B.V. All rights reserved.

Reliability of Laterality Effects in a Dichotic Listening Task with Words and Syllables

ERIC Educational Resources Information Center

Russell, Nancy L.; Voyer, Daniel

2004-01-01

Large and reliable laterality effects have been found using a dichotic target detection task in a recent experiment using word stimuli pronounced with an emotional component. The present study tested the hypothesis that the magnitude and reliability of the laterality effects would increase with the removal of the emotional component and variations…

Numerical simulation of hydrothermal circulation in the Cascade Range, north-central Oregon

USGS Publications Warehouse

Ingebritsen, S.E.; Paulson, K.M.

1990-01-01

Alternate conceptual models to explain near-surface heat-flow observations in the central Oregon Cascade Range involve (1) an extensive mid-crustal magmatic heat source underlying both the Quaternary arc and adjacent older rocks or (2) a narrower deep heat source which is flanked by a relatively shallow conductive heat-flow anomaly caused by regional ground-water flow (the lateral-flow model). Relative to the mid-crustal heat source model, the lateral-flow model suggests a more limited geothermal resource base, but a better-defined exploration target. We simulated ground-water flow and heat transport through two cross sections trending west from the Cascade range crest in order to explore the implications of the two models. The thermal input for the alternate conceptual models was simulated by varying the width and intensity of a basal heat-flow anomaly and, in some cases, by introducing shallower heat sources beneath the Quaternary arc. Near-surface observations in the Breitenbush Hot Springs area are most readily explained in terms of lateral heat transport by regional ground-water flow; however, the deep thermal structure still cannot be uniquely inferred. The sparser thermal data set from the McKenzie River area can be explained either in terms of deep regional ground-water flow or in terms of a conduction-dominated system, with ground-water flow essentially confined to Quaternary rocks and fault zones.

Flow and Temperature Distribution Evaluation on Sodium Heated Large-sized Straight Double-wall-tube Steam Generator

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kisohara, Naoyuki; Moribe, Takeshi; Sakai, Takaaki

2006-07-01

The sodium heated steam generator (SG) being designed in the feasibility study on commercialized fast reactor cycle systems is a straight double-wall-tube type. The SG is large sized to reduce its manufacturing cost by economics of scale. This paper addresses the temperature and flow multi-dimensional distributions at steady state to obtain the prospect of the SG. Large-sized heat exchanger components are prone to have non-uniform flow and temperature distributions. These phenomena might lead to tube buckling or tube to tube-sheet junction failure in straight tube type SGs, owing to tubes thermal expansion difference. The flow adjustment devices installed in themore » SG are optimized to prevent these issues, and the temperature distribution properties are uncovered by analysis methods. The analysis model of the SG consists of two parts, a sodium inlet distribution plenum (the plenum) and a heat transfer tubes bundle region (the bundle). The flow and temperature distributions in the plenum and the bundle are evaluated by the three-dimensional code 'FLUENT' and the two dimensional thermal-hydraulic code 'MSG', respectively. The MSG code is particularly developed for sodium heated SGs in JAEA. These codes have revealed that the sodium flow is distributed uniformly by the flow adjustment devices, and that the lateral tube temperature distributions remain within the allowable temperature range for the structural integrity of the tubes and the tube to tube-sheet junctions. (authors)« less

Method and apparatus for measuring flow velocity using matched filters

DOEpatents

Raptis, Apostolos C.

1983-01-01

An apparatus and method for measuring the flow velocities of individual phase flow components of a multiphase flow utilizes matched filters. Signals arising from flow noise disturbance are extracted from the flow, at upstream and downstream locations. The signals are processed through pairs of matched filters which are matched to the flow disturbance frequency characteristics of the phase flow component to be measured. The processed signals are then cross-correlated to determine the transit delay time of the phase flow component between sensing positions.

Rapid Salmonella detection in experimentally inoculated equine faecal and veterinary hospital environmental samples using commercially available lateral flow immunoassays.

PubMed

Burgess, B A; Noyes, N R; Bolte, D S; Hyatt, D R; van Metre, D C; Morley, P S

2015-01-01

Salmonella enterica is the most commonly reported cause of outbreaks of nosocomial infections in large animal veterinary teaching hospitals and the closure of equine hospitals. Rapid detection may facilitate effective control practices in equine populations. Shipping and laboratory testing typically require ≥48 h to obtain results. Lateral flow immunoassays developed for use in food-safety microbiology provide an alternative that has not been evaluated for use with faeces or environmental samples. We aimed to identify enrichment methods that would allow commercially available rapid Salmonella detection systems (lateral flow immunoassays) to be used in clinical practice with equine faecal and environmental samples, providing test results in 18-24 h. In vitro experiment. Equine faecal and environmental samples were inoculated with known quantities of S. enterica serotype Typhimurium and cultured using 2 different enrichment techniques for faeces and 4 enrichment techniques for environmental samples. Samples were tested blindly using 2 different lateral flow immunoassays and plated on agar media for confirmatory testing. In general, commercial lateral flow immunoassays resulted in fewer false-negative test results with enrichment of 1 g faecal samples in tetrathionate for 18 h, while all environmental sample enrichment techniques resulted in similar detection rates. The limit of detection from spiked samples, ∼4 colony-forming units/g, was similar for all methods evaluated. The lateral flow immunoassays evaluated could reliably detect S. enterica within 18 h, indicating that they may be useful for rapid point-of-care testing in equine practice applications. Additional evaluation is needed using samples from naturally infected cases and the environment to gain an accurate estimate of test sensitivity and specificity and to substantiate further the true value of these tests in clinical practice. © 2014 EVJ Ltd.

A high-resolution land model coupled with groundwater lateral flow, human water regulation and the changes in soil freeze-thaw fronts

NASA Astrophysics Data System (ADS)

Xie, Z.; Zeng, Y.; Liu, S.; Gao, J.; Jia, B.; Qin, P.

2017-12-01

Both anthropogenic water regulation and groundwater lateral flow essentially affect groundwater table patterns. Their relationship is close because lateral flow recharges the groundwater depletion cone, which is induced by over-exploitation. And the movement of frost and thaw fronts (FTFs) affects soil water and thermal characteristics, as well as energy and water exchanges between land surface and the atmosphere. In this study, schemes describing groundwater lateral flow, human water regulation and the changes in soil freeze-thaw fronts were developed and incorporated into the Community Land Model 4.5. Then the model was applied in Heihe River Basin(HRB), an arid and semiarid region, northwest China. High resolution ( 1 km) numerical simulations showed that groundwater lateral flow driven by changes in water heads can essentially change the groundwater table pattern with the deeper water table appearing in the hillslope regions and shallower water table appearing in valley bottom regions and plains. Over the last decade, anthropogenic groundwater exploitation deepened the water table by approximately 2 m in the middle reaches of the HRB and rapidly reduced the terrestrial water storage, while irrigation increased soil moisture by approximately 0.1 m3 m-3. The water stored in the mainstream of the Heihe River was also reduced by human surface water withdrawal. The latent heat flux was increased by 30 W m-2 over the irrigated region, with an identical decrease in sensible heat flux. The simulated groundwater lateral flow was shown to effectively recharge the groundwater depletion cone caused by over-exploitation. The offset rate is higher in plains than mountainous regions. In addition, the simulated FTFs depth compared well with the observed data both in D66 station (permafrost) and Hulugou station (seasonally frozen ground). Over the HRB, the upstream area is permafrost region with maximum thawed depth at 2.5 m and lower region is seasonal frozen ground region with maximum frozen depth at 3 m.

Vertebrate Left-Right Asymmetry: What Can Nodal Cascade Gene Expression Patterns Tell Us?

PubMed

Schweickert, Axel; Ott, Tim; Kurz, Sabrina; Tingler, Melanie; Maerker, Markus; Fuhl, Franziska; Blum, Martin

2017-12-29

Laterality of inner organs is a wide-spread characteristic of vertebrates and beyond. It is ultimately controlled by the left-asymmetric activation of the Nodal signaling cascade in the lateral plate mesoderm of the neurula stage embryo, which results from a cilia-driven leftward flow of extracellular fluids at the left-right organizer. This scenario is widely accepted for laterality determination in wildtype specimens. Deviations from this norm come in different flavors. At the level of organ morphogenesis, laterality may be inverted (situs inversus) or non-concordant with respect to the main body axis (situs ambiguus or heterotaxia). At the level of Nodal cascade gene activation, expression may be inverted, bilaterally induced, or absent. In a given genetic situation, patterns may be randomized or predominantly lacking laterality (absence or bilateral activation). We propose that the distributions of patterns observed may be indicative of the underlying molecular defects, with randomizations being primarily caused by defects in the flow-generating ciliary set-up, and symmetrical patterns being the result of impaired flow sensing, on the left, the right, or both sides. This prediction, the reasoning of which is detailed in this review, pinpoints functions of genes whose role in laterality determination have remained obscure.

Water in the critical zone: soil, water and life from profile to planet

NASA Astrophysics Data System (ADS)

Kirkby, Mike

2015-04-01

Water is essential to the critical zone between bedrock and the atmosphere, and without water the soil is dead. Water provides the basis for the abundant life within the soil and, interacting with micro-organisms, drives the key processes in the critical zone. This review looks at the balances that control the flow of water through the soil, and how water movement is one of the major controls on the fluxes and transformations that control the formation, evolution and loss of material that controls the 'life' and 'health' of the soil. At regional scales, climate, acting largely through the soil hydrology, plays a major part in determining the type of soils developed - from hyper arid soils dominated by aeolian inputs, through arid and semi-arid soils with largely vertical water exchanges with the atmosphere, to temperate soils with substantial lateral drainage, and humid soils dominated by organic peats. Soil water balance controls the partition of precipitation between evaporative loss, lateral subsurface flow and groundwater recharge, and, in turn, has a major influence on the potential for plant growth and on the lateral connectivity between soils on a hillslope. Sediment and solute balances distinguish soils of accumulation from soils that tend towards a stable chemical depletion ratio. Reflecting the availability of water and the soil material, carbon balance plays a major role in soil horizonation and distinguishes soils dominated by mineral or organic components. At finer catena and catchment scales, lateral connectivity, or its absence, determines how soils evolve through the transfer of water and sediment downslope, creating more or less integrated landscapes in a balance between geomorphological and pedological processes. Within single soil profiles, the movement of water controls the processes of weathering and soil horizonation by ion diffusion, advective leaching and bioturbation, creating horizonation that, in turn, modifies the hydrological responses of both soil and landscape. For example, the soil hydrological regime helps to contrast soils that accumulate more and less soluble constituents of the parent material.

Performance, Stability, and Control Investigation at Mach Numbers from 0.60 to 1.05 of a Model of the "Swallow" with Outer Wing Panels Swept 75 degree with and without Power Simulations

NASA Technical Reports Server (NTRS)

Schmeer, James W.; Cassetti, Marlowe D.

1960-01-01

An investigation of the performance, stability, and control characteristics of a variable-sweep arrow-wing model with the outer wing panels swept 75 deg. has been conducted in the Langley 16-foot transonic tunnel. Four outboard engines located above and below the wing provided propulsive thrust, and, by deflecting in the pitch direction and rotating in the lateral plane, also produced control forces. The engine nacelles incorporated swept lateral and vertical fins for aerodynamic stability and control. Jet-off data were obtained with flow-through nacelles, simulating inlet flow; jet thrust and hot-jet interference effects were obtained with faired-nose nacelles housing hydrogen peroxide gas generators. Six-component force and moment data were obtained at Mach numbers from 0.60 to 1.05 through a range of angles of attack and angles of side-slip. Control characteristics were obtained by deflecting the nacelle-fin combinations as elevators, rudders, and ailerons at several fixed angles for each control. The results indicate that the basic wing-body configuration becomes neutrally stable or unstable at a lift coefficient of 0.15; addition of nacelles with fins delayed instability to a lift coefficient of 0.30. Addition of nacelles to the wing-body configuration increased minimum drag from 0.0058 to 0.0100 at a Mach number of 0.60 and from 0.0080 to 0.0190 at a Mach number of 1.05 with corresponding reductions in maximum lift-drag ratio of 12 percent and 33 percent, respectively. The nacelle-fin combinations were ineffective as longitudinal controls but were adequate as directional and lateral controls. The model with nacelles and fins was directionally and laterally stable; the stability generally increased with increasing lift. Jet interference effects on stability and control characteristics were small but the adverse effects on drag were greater than would be expected for isolated nacelles.

Does Kinematic Alignment and Flexion of a Femoral Component Designed for Mechanical Alignment Reduce the Proximal and Lateral Reach of the Trochlea?

PubMed

Brar, Abheetinder S; Howell, Stephen M; Hull, Maury L; Mahfouz, Mohamed R

2016-08-01

Kinematically aligned total knee arthroplasty uses a femoral component designed for mechanical alignment (MA) and sets the component in more internal, valgus, and flexion rotation than MA. It is unknown how much kinematic alignment (KA) and flexion of the femoral component reduce the proximal and lateral reach of the trochlea; two reductions that could increase the risk of abnormal patella tracking. We simulated MA and KA of the femoral component in 0° of flexion on 20 3-dimensional bone models of normal femurs. The mechanically and kinematically aligned components were then aligned in 5°, 10°, and 15° of flexion and downsized until the flange contacted the anterior femur. The reductions in the proximal and lateral reach from the proximal point of the trochlea of the MA component set in 0° of flexion were computed. KA at 0° of flexion did not reduce the proximal reach and reduced the lateral reach an average of 3 mm. Flexion of the MA and KA femoral component 5°, 10°, and 15° reduced the proximal reach an average of 4 mm, 8 mm, and 12 mm, respectively (0.8 mm/degree of flexion), and reduced the lateral reach an average of 1 mm and 4 mm regardless of the degree of flexion, respectively. Arthroplasty surgeons and biomechanical engineers striving to optimize patella tracking might consider developing surgical techniques to minimize flexion of the femoral component when performing KA and MA total knee arthroplasty to promote early patella engagement and consider designing a femoral component with a trochlea shaped specifically for KA. Copyright © 2016 Elsevier Inc. All rights reserved.

3D Modeling of the Deep Groundwater System at Mount Shasta, California, Using Finite Difference and Inverse Modeling in Combination with Magnetotellurics

NASA Astrophysics Data System (ADS)

Webb, C. H.; Foglia, L.; Fogg, G. E.; McClain, J.

2017-12-01

Precipitation in mountainous systems is responsible for much of the world's freshwater supply. Volcanic mountains in particular may have the capacity to store large amounts of groundwater, due to the relatively high permeability of volcanic rocks as compared to fractured crystalline rocks. These qualities make volcanic aquifers likely candidates for laterally extensive deep groundwater systems. However, the depth extent of these aquifers is not well understood and has been little studied, due to the dearth of well data in most mountain systems. When determining a water budget, especially for mountainous regions, it is necessary to understand the extent of the entire system, including the deep components. Mount Shasta of the California cascade volcanoes is one potential case of a deep groundwater system with the capacity to store significant amounts of water. In order to develop a conceptual model of the role of deep and regional groundwater flow in the Mt. Shasta groundwater system, the region was modeled using MODFLOW_2005, the finite difference flow model developed by USGS. The model was constrained using SRTM topography data, spring flow rates, PRISM precipitation rates, and well log levels. Geologic cross sections and gravity data were referenced in order to create a realistic estimate of the aquifer's structure down to 6km in depth. The aquifer stratigraphy was then represented by using 6 layers with 2-4 zones of hydraulic conductivity per layer to account for both vertical and lateral differences in lithology as well as decreasing permeability with depth. These hydraulic conductivity parameters of the model were varied using inverse modeling (UCODE_2014) to determine which layers and zones could support flow and still produce results consistent with existing well logs. Depth of flow was also corroborated with resistivity data collected in Shasta Valley using magnetotelluric (MT) soundings. Depths with comparatively low electrical resistivity were assumed to be aquifer units, and zones with high resistivity were assumed to be aquitards. By performing MT soundings in multiple locations and dividing the model into zones, this model tests both the maximum depth of flow as well as how that depth varies with lithology and geographical location.

Calculations of air cooler for new subsonic wind tunnel

NASA Astrophysics Data System (ADS)

Rtishcheva, A. S.

2017-10-01

As part of the component development of TsAGI’s new subsonic wind tunnel where the air flow velocity in the closed test section is up to 160 m/sec hydraulic and thermal characteristics of air cooler are calculated. The air cooler is one of the most important components due to its highest hydraulic resistance in the whole wind tunnel design. It is important to minimize its hydraulic resistance to ensure the energy efficiency of wind tunnel fans and the cost-cutting of tests. On the other hand the air cooler is to assure the efficient cooling of air flow in such a manner as to maintain the temperature below 40 °C for seamless operation of measuring equipment. Therefore the relevance of this project is driven by the need to develop the air cooler that would demonstrate low hydraulic resistance of air and high thermal effectiveness of heat exchanging surfaces; insofar as the cooling section must be given up per unit time with the amount of heat Q=30 MW according to preliminary evaluations. On basis of calculation research some variants of air cooler designs are proposed including elliptical tubes, round tubes, and lateral plate-like fins. These designs differ by the number of tubes and plates, geometrical characteristics and the material of finned surfaces (aluminium or cooper). Due to the choice of component configurations a high thermal effectiveness is achieved for finned surfaces. The obtained results form the basis of R&D support in designing the new subsonic wind tunnel.

High frequency lateral flow affinity assay using superparamagnetic nanoparticles

NASA Astrophysics Data System (ADS)

Lago-Cachón, D.; Rivas, M.; Martínez-García, J. C.; Oliveira-Rodríguez, M.; Blanco-López, M. C.; García, J. A.

2017-02-01

Lateral flow assay is one of the simplest and most extended techniques in medical diagnosis for point-of-care testing. Although it has been traditionally a positive/negative test, some work has been lately done to add quantitative abilities to lateral flow assay. One of the most successful strategies involves magnetic beads and magnetic sensors. Recently, a new technique of superparamagnetic nanoparticle detection has been reported, based on the increase of the impedance induced by the nanoparticles on a RF-current carrying copper conductor. This method requires no external magnetic field, which reduces the system complexity. In this work, nitrocellulose membranes have been installed on the sensor, and impedance measurements have been carried out during the sample diffusion by capillarity along the membrane. The impedance of the sensor changes because of the presence of magnetic nanoparticles. The results prove the potentiality of the method for point-of-care testing of biochemical substances and nanoparticle capillarity flow studies.

Model space exploration for determining landslide source history from long period seismic data

NASA Astrophysics Data System (ADS)

Zhao, Juan; Mangeney, Anne; Stutzmann, Eléonore; Capdeville, Yann; Moretti, Laurent; Calder, Eliza S.; Smith, Patrick J.; Cole, Paul; Le Friant, Anne

2013-04-01

The seismic signals generated by high magnitude landslide events can be recorded at remote stations, which provides access to the landslide process. During the "Boxing Day" eruption at Montserrat in 1997, the long period seismic signals generated by the debris avalanche are recorded by two stations at distances of 450 km and 1261 km. We investigate the landslide process considering that the landslide source can be described by single forces. The period band 25-50 sec is selected for which the landslide signal is clearly visible at the two stations. We first use the transverse component of the closest station to determine the horizontal forces. We model the seismogram by normal mode summation and investigate the model space. Two horizontal forces are found that best fit the data. These two horizontal forces have similar amplitude, but opposite direction and they are separated in time by 70 sec. The radiation pattern of the transverse component does not enable to determine the exact azimuth of these forces. We then model the vertical component of the seismograms which enable to retrieve both the vertical and horizontal forces. Using the parameter previously determined (amplitude ratio and time shift of the 2 horizontal forces), we further investigate the model space and show that a single vertical force together with the 2 horizontal forces enable to fit the data. The complete source time function can be described as follows: a horizontal force toward the opposite direction of the landslide flow is followed 40 sec later by a vertical downward force and 30 more seconds later by a horizontal force toward the direction of the flow. Inverting directly the seismograms in the period band 25-50sec enable to retrieve a source time function that is consistent with the 3 forces determined previously. The source time function in this narrow period band alone does not enable easily to recover the corresponding single forces. This method can be used to determine the source parameters using only 2 distant stations. It is successfully tested also on Mount St. Helens (1980) event which are recorded by more broadband stations.

Topographically driven crustal flow and its implication to the development of pinned oroclines

NASA Technical Reports Server (NTRS)

Hsui, Albert T.; Wilkerson, M. Scott; Marshak, Stephen

1990-01-01

Pinned oroclines, a type of curved orogen which results from lateral pinning of a growing fold-thrust belt, tend to resemble parabolic Newtonian curvature modified by different degrees of flattening at the flow front. It is proposed that such curves can be generated by Newtonian crustal flow driven by topographic variations. In this model, regional topographic differences create a regional flow which produces a parabolic flow front on interaction with lateral bounding obstacles. Local topographic variations modify the parabolic curves and yield more flat-crested, non-Newtonian-type curvatures. A finite-difference thin-skin tectonic simulation demonstrates that both Newtonian and non-Newtonian curved orogens can be produced within a Newtonian crust.

Thermally determining flow and/or heat load distribution in parallel paths

DOE Office of Scientific and Technical Information (OSTI.GOV)

Chainer, Timothy J.; Iyengar, Madhusudan K.; Parida, Pritish R.

A method including obtaining calibration data for at least one sub-component in a heat transfer assembly, wherein the calibration data comprises at least one indication of coolant flow rate through the sub-component for a given surface temperature delta of the sub-component and a given heat load into said sub-component, determining a measured heat load into the sub-component, determining a measured surface temperature delta of the sub-component, and determining a coolant flow distribution in a first flow path comprising the sub-component from the calibration data according to the measured heat load and the measured surface temperature delta of the sub-component.

Thermally determining flow and/or heat load distribution in parallel paths

DOEpatents

Chainer, Timothy J.; Iyengar, Madhusudan K.; Parida, Pritish R.

2016-12-13

A method including obtaining calibration data for at least one sub-component in a heat transfer assembly, wherein the calibration data comprises at least one indication of coolant flow rate through the sub-component for a given surface temperature delta of the sub-component and a given heat load into said sub-component, determining a measured heat load into the sub-component, determining a measured surface temperature delta of the sub-component, and determining a coolant flow distribution in a first flow path comprising the sub-component from the calibration data according to the measured heat load and the measured surface temperature delta of the sub-component.

Evaluation of a lateral flow immunoassay for field identification of Solenopsis invicta (Hymenoptera: Formicidae) in Australia

USDA-ARS?s Scientific Manuscript database

In an effort to improve surveillance capacity for the exotic red imported fire ant, Solenopsis invicta, a lateral flow immunoassay (LFA) was recently evaluated by Biosecurity Queensland staff in Australia. The purpose of the research was to assess the ability of the fire ant LFA to discriminate S. i...

The performance characteristics of lateral flow devices with 2 strains of highly pathogenic avian influenza virus

USDA-ARS?s Scientific Manuscript database

Lateral flow devices (LFD) are commercially available and provide a fast, highly specific, on-site test for avian influenza. Because of the low analytic sensitivity of LFD tests at low virus concentrations, targeted sampling of sick and dead birds has been proposed in order to increase detection pr...

Method and apparatus for monitoring characteristics of a flow path having solid components flowing therethrough

DOEpatents

Hoskinson, Reed L [Rigby, ID; Svoboda, John M [Idaho Falls, ID; Bauer, William F [Idaho Falls, ID; Elias, Gracy [Idaho Falls, ID

2008-05-06

A method and apparatus is provided for monitoring a flow path having plurality of different solid components flowing therethrough. For example, in the harvesting of a plant material, many factors surrounding the threshing, separating or cleaning of the plant material and may lead to the inadvertent inclusion of the component being selectively harvested with residual plant materials being discharged or otherwise processed. In accordance with the present invention the detection of the selectively harvested component within residual materials may include the monitoring of a flow path of such residual materials by, for example, directing an excitation signal toward of flow path of material and then detecting a signal initiated by the presence of the selectively harvested component responsive to the excitation signal. The detected signal may be used to determine the presence or absence of a selected plant component within the flow path of residual materials.

Method and apparatus for measuring flow velocity using matched filters

DOEpatents

Raptis, A.C.

1983-09-06

An apparatus and method for measuring the flow velocities of individual phase flow components of a multiphase flow utilizes matched filters. Signals arising from flow noise disturbance are extracted from the flow, at upstream and downstream locations. The signals are processed through pairs of matched filters which are matched to the flow disturbance frequency characteristics of the phase flow component to be measured. The processed signals are then cross-correlated to determine the transit delay time of the phase flow component between sensing positions. 8 figs.

Mise en oeuvre et etalonnage d'une soufflerie de recherche sur les couches limites

NASA Astrophysics Data System (ADS)

Pioton, Julien

In order to reproduce a turbulent boundary layer separation bubble in a laboratory at the École de Technologie Supérieure of Montreal, an open-circuit wind tunnel equipped with a blow-down centrifugal fan has been implemented and calibrated for this research project. The methodology for the conception of the wind tunnel components, based on the results obtained from previous experiments, has been validated by the comparison between experimental and theoretical pressure losses along the wind tunnel. Dimensionless mean axial velocity measurements in the working section have showed an irrotational uniform flow zone at the contraction end, which demonstrates the satisfactory performance of the flowconditioning components located upstream from the working section. Mean axial velocity and total pressure measurements along the working section have allowed for estimates of the location of boundary between irrotational and rotational flow at the separation bubble. The maximum mean height of the boundary layer has been estimated at around 15 cm. Oil film visualisations have revealed a mean bubble separation length of approximately 52 cm. Theses oil visualisations, supported by mean lateral velocity measurements in the current configuration of the working section, have indicated important three-dimensional effects and significant dissymmetry in the vicinity of the separation and reattachment zones at the separation bubble.

Linking behavior, physiology, and survival of Atlantic Salmon smolts during estuary migration

USGS Publications Warehouse

Stich, Daniel S.; Zydlewski, Gayle B.; Kocik, John F.; Zydlewski, Joseph D.

2015-01-01

Decreased marine survival is identified as a component driver of continued declines of Atlantic Salmon Salmo salar. However, estimates of marine mortality often incorporate loss incurred during estuary migration that may be mechanistically distinct from factors affecting marine mortality. We examined movements and survival of 941 smolts (141 wild and 800 hatchery-reared fish) released in freshwater during passage through the Penobscot River estuary, Maine, from 2005 to 2013. We related trends in estuary arrival date, movement rate, and survival to fish characteristics, migratory history, and environmental conditions in the estuary. Fish that experienced the warmest thermal history arrived in the estuary 8 d earlier than those experiencing the coolest thermal history during development. Estuary arrival date was 10 d later for fish experiencing high flow than for fish experiencing low flow. Fish released furthest upstream arrived in the estuary 3 d later than those stocked further downstream but moved 0.5 km/h faster through the estuary. Temporally, movement rate and survival in the estuary both peaked in mid-May. Spatially, movement rate and survival both decreased from freshwater to the ocean. Wild smolts arrived in the estuary later than hatchery fish, but we observed no change in movement rate or survival attributable to rearing history. Fish with the highest gill Na+, K+-ATPase activity incurred 25% lower mortality through the estuary than fish with the lowest gill Na+, K+-ATPase activity. Smolt survival decreased (by up to 40%) with the increasing number of dams passed (ranging from two to nine) during freshwater migration. These results underscore the importance of physiological preparedness on performance and the delayed, indirect effects of dams on survival of Atlantic Salmon smolts during estuary migration, ultimately affecting marine survival estimates.

Low-Cost 3-D Flow Estimation of Blood With Clutter.

PubMed

Wei, Siyuan; Yang, Ming; Zhou, Jian; Sampson, Richard; Kripfgans, Oliver D; Fowlkes, J Brian; Wenisch, Thomas F; Chakrabarti, Chaitali

2017-05-01

Volumetric flow rate estimation is an important ultrasound medical imaging modality that is used for diagnosing cardiovascular diseases. Flow rates are obtained by integrating velocity estimates over a cross-sectional plane. Speckle tracking is a promising approach that overcomes the angle dependency of traditional Doppler methods, but suffers from poor lateral resolution. Recent work improves lateral velocity estimation accuracy by reconstructing a synthetic lateral phase (SLP) signal. However, the estimation accuracy of such approaches is compromised by the presence of clutter. Eigen-based clutter filtering has been shown to be effective in removing the clutter signal; but it is computationally expensive, precluding its use at high volume rates. In this paper, we propose low-complexity schemes for both velocity estimation and clutter filtering. We use a two-tiered motion estimation scheme to combine the low complexity sum-of-absolute-difference and SLP methods to achieve subpixel lateral accuracy. We reduce the complexity of eigen-based clutter filtering by processing in subgroups and replacing singular value decomposition with less compute-intensive power iteration and subspace iteration methods. Finally, to improve flow rate estimation accuracy, we use kernel power weighting when integrating the velocity estimates. We evaluate our method for fast- and slow-moving clutter for beam-to-flow angles of 90° and 60° using Field II simulations, demonstrating high estimation accuracy across scenarios. For instance, for a beam-to-flow angle of 90° and fast-moving clutter, our estimation method provides a bias of -8.8% and standard deviation of 3.1% relative to the actual flow rate.

Salt tectonics and shallow subseafloor fluid convection: Models of coupled fluid-heat-salt transport

USGS Publications Warehouse

Wilson, A.; Ruppel, C.

2007-01-01

Thermohaline convection associated with salt domes has the potential to drive significant fluid flow and mass and heat transport in continental margins, but previous studies of fluid flow associated with salt structures have focused on continental settings or deep flow systems of importance to petroleum exploration. Motivated by recent geophysical and geochemical observations that suggest a convective pattern to near-seafloor pore fluid flow in the northern Gulf of Mexico (GoMex), we devise numerical models that fully couple thermal and chemical processes to quantify the effects of salt geometry and seafloor relief on fluid flow beneath the seafloor. Steady-state models that ignore halite dissolution demonstrate that seafloor relief plays an important role in the evolution of shallow geothermal convection cells and that salt at depth can contribute a thermal component to this convection. The inclusion of faults causes significant, but highly localized, increases in flow rates at seafloor discharge zones. Transient models that include halite dissolution show the evolution of flow during brine formation from early salt-driven convection to later geothermal convection, characteristics of which are controlled by the interplay of seafloor relief and salt geometry. Predicted flow rates are on the order of a few millimeters per year or less for homogeneous sediments with a permeability of 10−15 m2, comparable to compaction-driven flow rates. Sediment permeabilities likely fall below 10−15 m2 at depth in the GoMex basin, but such thermohaline convection can drive pervasive mass transport across the seafloor, affecting sediment diagenesis in shallow sediments. In more permeable settings, such flow could affect methane hydrate stability, seafloor chemosynthetic communities, and the longevity of fluid seeps.

Sheathless Size-Based Acoustic Particle Separation

PubMed Central

Guldiken, Rasim; Jo, Myeong Chan; Gallant, Nathan D.; Demirci, Utkan; Zhe, Jiang

2012-01-01

Particle separation is of great interest in many biological and biomedical applications. Flow-based methods have been used to sort particles and cells. However, the main challenge with flow based particle separation systems is the need for a sheath flow for successful operation. Existence of the sheath liquid dilutes the analyte, necessitates precise flow control between sample and sheath flow, requires a complicated design to create sheath flow and separation efficiency depends on the sheath liquid composition. In this paper, we present a microfluidic platform for sheathless particle separation using standing surface acoustic waves. In this platform, particles are first lined up at the center of the channel without introducing any external sheath flow. The particles are then entered into the second stage where particles are driven towards the off-center pressure nodes for size based separation. The larger particles are exposed to more lateral displacement in the channel due to the acoustic force differences. Consequently, different-size particles are separated into multiple collection outlets. The prominent feature of the present microfluidic platform is that the device does not require the use of the sheath flow for positioning and aligning of particles. Instead, the sheathless flow focusing and separation are integrated within a single microfluidic device and accomplished simultaneously. In this paper, we demonstrated two different particle size-resolution separations; (1) 3 μm and 10 μm and (2) 3 μm and 5 μm. Also, the effects of the input power, the flow rate, and particle concentration on the separation efficiency were investigated. These technologies have potential to impact broadly various areas including the essential microfluidic components for lab-on-a-chip system and integrated biological and biomedical applications. PMID:22368502

Improved crystalline quality of AlN epitaxial layer on sapphire by introducing TMGa pulse flow into the nucleation stage

NASA Astrophysics Data System (ADS)

Wu, Hualong; Wang, Hailong; Chen, Yingda; Zhang, Lingxia; Chen, Zimin; Wu, Zhisheng; Wang, Gang; Jiang, Hao

2018-05-01

The crystalline quality of AlN epitaxial layers on sapphire substrates was improved by introducing trimethylgallium (TMGa) pulse flow into the growth of AlN nucleation layers. It was found that the density of both screw- and edge-type threading dislocations could be significantly reduced by introducing the TMGa pulse flow. With increasing TMGa pulse flow times, the lateral correlation length (i.e. the grain size) increases and the strain in the AlN epilayers changes from tensile state to compressive state. Unstrained AlN with the least dislocations and a smooth surface was obtained by introducing 2-times TMGa pulse flow. The crystalline improvement is attributed to enhanced lateral growth and improved crystalline orientation by the TMGa pulse flow.

Paleomagnetic quantification of upper-plate deformation during Miocene detachment faulting in the Mohave Mountains, Arizona

USGS Publications Warehouse

Pease, V.; Hillhouse, J.W.; Wells, R.E.

2005-01-01

Paleomagnetic data from Miocene (???20 Ma) volcanic rocks and dikes of west central Arizona reveal the tilt history of Proterozoic crystalline rocks in the hanging wall of the Chemehuevi-Whipple Mountains detachment fault. We obtained magnetization data from dikes and flows in two structural blocks encompassing Crossman Peak and Standard Wash in the Mohave Mountains. In the Crossman block the dike swarm records two components of primary magnetization: (1) CNH, a normal polarity, high-unblocking-temperature or high-coercivity component (inclination, I = 48.5??, declination, D = 6.4??), and (2) CRHm, a reversed polarity, high-temperature or high-coercivity component (I = -33.6??, D = 197.5??). Argon age spectra imply that the dikes have not been reheated above 300??C since their emplacement, and a baked-contact test suggests that the magnetization is likely to be Miocene in age. CRHm deviates from the expected direction of the Miocene axial dipole field and is best explained as a result of progressive tilting about the strike of the overlying andesite flows. These data suggest that the Crossman block was tilted 60?? to the southwest prior to intrusion of the vertical dike swarm, and the block continued to tilt during a magnetic field reversal to normal polarity (CNH). Miocene dikes in the Crossman block are roughly coplanar, so the younger dikes with normal polarity magnetization intruded along planes of weakness parallel to the earlier reversed polarity swarm. An alternative explanation involves CNH magnetization being acquired later during hydrothermal alteration associated with the final stages of dike emplacement. In the Standard Wash block, the primary component of magnetization is a dual-polarity, high-temperature or high-coercivity component (SWHl, I = 7.2??,D= 0.7??). To produce agreement between the expected Miocene magnetic direction and the SWH component requires (1) correcting for a 56?? tilt about the strike of flow bedding and (2) removing a counterclockwise vertical-axis rotation of 20??. The two rotations restore the Standard Wash dikes to vertical, make parallel the dike layering in the Crossman and Standard Wash blocks, and align the strikes of bedding in both blocks. Geologic mapping, geochemical evidence, and paleomagnetic data are consistent with the upper plate of the Mohave Mountains having tilted in response to formation of the underlying detachment fault.

Evidence of spatial and temporal slip partitioning in the northern Central Nevada Seismic Belt from ground-based imaging of offset landforms

NASA Astrophysics Data System (ADS)

Gold, P. O.; Cowgill, E.; Kreylos, O.

2010-12-01

Measurements derived from high-resolution terrestrial LiDAR (t-Lidar) surveys of landforms displaced during the 16 December 1954 Mw 6.8 Dixie Valley earthquake in central Nevada confirm the absence of historical strike slip north of latitude 39.5°N. This conclusion has implications for the effect of stress changes on the spatial and temporal evolution of the central Nevada seismic belt. The Dixie Valley fault is a low-angle, east-dipping, range-bounding normal fault located in the central-northern reach of the central Nevada seismic belt (CNSB), a ~N-S trending group of historical ruptures that may represent a migration of northwest trending right-lateral Pacific-North American plate motion into central Nevada. Migration of a component of right slip eastward from the eastern California shear zone/Walker lane to the CNSB is supported by the presence of pronounced right-lateral motion observed in most of the CNSB earthquakes south of the Dixie Valley fault and by GPS data spanning the CNSB. Such eastward migration and northward propagation of right-slip into the CNSB predicts a component of lateral slip on the Dixie Valley fault. However, landforms offsets have previously been reported to indicate only purely normal slip in the 1954 Dixie Valley event. To check the direction of motion during the Dixie Valley earthquake using higher precision methods than previously employed, we collected t-LiDAR data to quantify displacements of two well-preserved debris flow chutes separated along strike by ~10 km and at locations where the local fault strike diverges by >10° from the regional strike. Our highest confidence measurements yield a horizontal slip vector azimuth of ~107° at both sites, orthogonal to the average regional fault strike of ~17°. Thus, we find no compelling evidence for regional lateral motion in our other measurements. This result indicates that continued northward propagation of right lateral slip from its diffuse termination at the northern end of the 1954 Fairview Peak event, 4 minutes before the Dixie Valley event, and the Rainbow Mountain-Stillwater events six months earlier, must be accommodated by some other mechanism. We see several options for the spatial and temporal evolution of right slip propagation into the northern CNSB. 1) Lateral motion may be accommodated to the east by faults opposite the Dixie Valley fault along the base of Clan Alpine range, or to the west by faults at the western base of the Stillwater range-diffuse faults to the SW and SE of the Dixie Valley fault that also ruptured in 1954 accommodated right slip and could represent a west and/or east migration of lateral motion; 2) right lateral motion may activate an as yet unrecognized fault within the Dixie Valley; or 3) the Dixie Valley fault may be reactivated with a greater component of lateral slip in response to changes in stress, a phenomena that has been recognized on the Borrego Fault in northern Mexico between the penultimate event and the recent 4 April 2010 El Mayor-Cucapah earthquake.

An Investigation of the Performance of Various Reaction Control Devices

NASA Technical Reports Server (NTRS)

Hunter, Paul A.

1959-01-01

An investigation of a small-scale reaction control devices in still air with both subsonic and supersonic internal flows has shown that lateral forces approaching 70 percent of the resultant force of the undeflected jet can be obtained. These results were obtained with a tilted extension at a deflection of 40 deg. The tests of tilted extensions indicated an optimum length-to-diameter ratio of approximately 0.75 to 1.00, dependent upon the deflection angle. For the two geometric types of spoiler tabs tested, blockage-area ratio appears to be the only variable affecting the lateral force developed. Usable values of lateral force were developed by the full-eyelid type of device with reasonably small losses in the thrust and weight flow. Somewhat larger values of lateral force were developed by injecting a secondary flow normal to the primary jet, but for conditions of these tests the losses in thrust and weight flow were large. Relatively good agreement with other investigations was obtained for several of the devices. The agreement of the present results with those of an investigation made with larger-scale equipment indicates that Reynolds number may not be critical for these tests. In as much as the effects of external flow could influence the performance and other factors affecting the choice of a reaction control for a specific use, it would appear desirable to make further tests of the devices described in this report in the presence of external flow.

Variability of Rheotaxis Behaviors in Larval Bullfrogs Highlights Species Diversity in Lateral Line Function

PubMed Central

Brown, Erika E. A.

2016-01-01

The morphology and distribution of lateral line neuromasts vary between ecomorphological types of anuran tadpoles, but little is known about how this structural variability contributes to differences in lateral-line mediated behaviors. Previous research identified distinct differences in one such behavior, positive rheotaxis towards the source of a flow, in two tadpole species, the African clawed frog (Xenopus laevis; type 1) and the American bullfrog (Rana catesbeiana; type 4). Because these two species had been tested under different flow conditions, we re-evaluated these findings by quantifying flow-sensing behaviors of bullfrog tadpoles in the same flow field in which X. laevis tadpoles had been tested previously. Early larval bullfrog tadpoles were exposed to flow in the dark, in the presence of a discrete light cue, and after treatment with the ototoxin gentamicin. In response to flow, tadpoles moved downstream, closer to a side wall, and higher in the water column, but they did not station-hold. Tadpoles exhibited positive rheotaxis, but with long latencies, low to moderate accuracy, and considerable individual variability. This is in contrast to the robust, stereotyped station-holding and accurate rheotaxis of X. laevis tadpoles. The presence of a discrete visual cue and gentamicin treatment altered spatial positioning and disrupted rheotaxis in both tadpole species. Species differences in lateral-line mediated behaviors may reflect differences in neuromast number and distribution, life history, or perceptual salience of other environmental cues. PMID:27870909

Laboratory Testing Protocols for Heparin-Induced Thrombocytopenia (HIT) Testing.

PubMed

Lau, Kun Kan Edwin; Mohammed, Soma; Pasalic, Leonardo; Favaloro, Emmanuel J

2017-01-01

Heparin-induced thrombocytopenia (HIT) represents a significant high morbidity complication of heparin therapy. The clinicopathological diagnosis of HIT remains challenging for many reasons; thus, laboratory testing represents an important component of an accurate diagnosis. Although there are many assays available to assess HIT, these essentially fall into two categories-(a) immunological assays, and (b) functional assays. The current chapter presents protocols for several HIT assays, being those that are most commonly performed in laboratory practice and have the widest geographic distribution. These comprise a manual lateral flow-based system (STiC), a fully automated latex immunoturbidimetric assay, a fully automated chemiluminescent assay (CLIA), light transmission aggregation (LTA), and whole blood aggregation (Multiplate).

Replacing antibodies with aptamers in lateral flow immunoassay.

PubMed

Chen, Ailiang; Yang, Shuming

2015-09-15

Aptamers have been identified against various targets as a type of chemical or nucleic acid ligand by systematic evolution of ligands by exponential enrichment (SELEX) with high sensitivity and specificity. Aptamers show remarkable advantages over antibodies due to the nucleic acid nature and target-induced structure-switching properties and are widely used to design various fluorescent, electrochemical, or colorimetric biosensors. However, the practical applications of aptamer-based sensing and diagnostics are still lagging behind those of antibody-based tests. Lateral flow immunoassay (LFIA) represents a well established and appropriate technology among rapid assays because of its low cost and user-friendliness. The antibody-based platform is utilized to detect numerous targets, but it is always hampered by the antibody preparation time, antibody stability, and effect of modification on the antibody. Seeking alternatives to antibodies is an area of active research and is of tremendous importance. Aptamers are receiving increasing attention in lateral flow applications because of a number of important potential performance advantages. We speculate that aptamer-based LFIA may be one of the first platforms for commercial use of aptamer-based diagnosis. This review first gives an introduction to aptamer including the selection process SELEX with its focus on aptamer advantages over antibodies, and then depicts LFIA with its focus on aptamer opportunities in LFIA over antibodies. Furthermore, we summarize the recent advances in the development of aptamer-based lateral flow biosensing assays with the aim to provide a general guide for the design of aptamer-based lateral flow biosensing assays. Copyright © 2015 Elsevier B.V. All rights reserved.

A lateral flow biosensor for detection of single nucleotide polymorphism by circular strand displacement reaction.

PubMed

Xiao, Zhuo; Lie, Puchang; Fang, Zhiyuan; Yu, Luxin; Chen, Junhua; Liu, Jie; Ge, Chenchen; Zhou, Xuemeng; Zeng, Lingwen

2012-09-04

A lateral flow biosensor for detection of single nucleotide polymorphism based on circular strand displacement reaction (CSDPR) has been developed. Taking advantage of high fidelity of T4 DNA ligase, signal amplification by CSDPR, and the optical properties of gold nanoparticles, this assay has reached a detection limit of 0.01 fM.

Detection of H5 and H7 highly pathogenic avian influenza virus with lateral flow devices: performance with healthy, sick and dead chickens

USDA-ARS?s Scientific Manuscript database

Rapid detection of highly pathogenic avian influenza virus (HPAIV) in the field is critical for effective disease control and to differentiate it from other diseases, such as Newcastle disease. Lateral flow devices (LFD) are commercially available and provide a fast, highly specific, on-site test fo...

Rapid detection of Bacillus anthracis spores using a super-paramagnetic lateral-flow immunological detection system.

PubMed

Wang, Dian-Bing; Tian, Bo; Zhang, Zhi-Ping; Deng, Jiao-Yu; Cui, Zong-Qiang; Yang, Rui-Fu; Wang, Xu-Ying; Wei, Hong-Ping; Zhang, Xian-En

2013-04-15

There is an urgent need for convenient, sensitive, and specific methods to detect the spores of Bacillus anthracis, the causative agent of anthrax, because of the bioterrorism threat posed by this bacterium. In this study, we firstly develop a super-paramagnetic lateral-flow immunological detection system for B. anthracis spores. This system involves the use of a portable magnetic assay reader, super-paramagnetic iron oxide particles, lateral-flow strips and two different monoclonal antibodies directed against B. anthracis spores. This detection system specifically recognises as few as 400 pure B. anthracis spores in 30 min. This system has a linear range of 4×10³-10⁶ CFU ml⁻¹ and reproducible detection limits of 200 spores mg⁻¹ milk powder and 130 spores mg⁻¹ soil for simulated samples. In addition, this approach shows no obvious cross-reaction with other related Bacillus spores, even at high concentrations, and has no significant dependence on the duration of the storage of the immunological strips. Therefore, this super-paramagnetic lateral-flow immunological detection system is a promising tool for the rapid and sensitive detection of Bacillus anthracis spores under field conditions. Copyright © 2012 Elsevier B.V. All rights reserved.

Turboprop engine and method of operating the same

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klees, G.W.; Johnson, P.E.

1986-02-11

This patent describes a turboprop engine consisting of: 1.) A compressor; 2.) A turbine; 3.) A combustion section; 4.) A variable pitch propeller; 5.) A speed reducing transmission; 6.) An air inlet; 7.) An air inlet bypass; 8.) An air outlet bypass duct; 9.) A flow control operatively positioned to receive air flow from the air inlet bypass and air flow from the low pressure compressor component. To direct the air flow to the air outlet bypass duct, and the air flow to the high pressure compressor component, the flow control has a first position where the air flow ismore » from. The high and low pressure compressor components and is directed to the air outlet bypass duct. The flow control has a second position for the air flow from the air inlet bypass duct to the air outlet bypass duct and air from the low pressure compressor component is directed to the high pressure compressor component. A method of operating a turboprop engine.« less

Perception of object trajectory: parsing retinal motion into self and object movement components.

PubMed

Warren, Paul A; Rushton, Simon K

2007-08-16

A moving observer needs to be able to estimate the trajectory of other objects moving in the scene. Without the ability to do so, it would be difficult to avoid obstacles or catch a ball. We hypothesized that neural mechanisms sensitive to the patterns of motion generated on the retina during self-movement (optic flow) play a key role in this process, "parsing" motion due to self-movement from that due to object movement. We investigated this "flow parsing" hypothesis by measuring the perceived trajectory of a moving probe placed within a flow field that was consistent with movement of the observer. In the first experiment, the flow field was consistent with an eye rotation; in the second experiment, it was consistent with a lateral translation of the eyes. We manipulated the distance of the probe in both experiments and assessed the consequences. As predicted by the flow parsing hypothesis, manipulating the distance of the probe had differing effects on the perceived trajectory of the probe in the two experiments. The results were consistent with the scene geometry and the type of simulated self-movement. In a third experiment, we explored the contribution of local and global motion processing to the results of the first two experiments. The data suggest that the parsing process involves global motion processing, not just local motion contrast. The findings of this study support a role for optic flow processing in the perception of object movement during self-movement.

Predictable turn-around time for post tape-out flow

NASA Astrophysics Data System (ADS)

Endo, Toshikazu; Park, Minyoung; Ghosh, Pradiptya

2012-03-01

A typical post-out flow data path at the IC Fabrication has following major components of software based processing - Boolean operations before the application of resolution enhancement techniques (RET) and optical proximity correctin (OPC), the RET and OPC step [etch retargeting, sub-resolution assist feature insertion (SRAF) and OPC], post-OPCRET Boolean operations and sometimes in the same flow simulation based verification. There are two objectives that an IC Fabrication tapeout flow manager wants to achieve with the flow - predictable completion time and fastest turn-around time (TAT). At times they may be competing. There have been studies in the literature modeling the turnaround time from historical data for runs with the same recipe and later using that to derive the resource allocation for subsequent runs. [3]. This approach is more feasible in predominantly simulation dominated tools but for edge operation dominated flow it may not be possible especially if some processing acceleration methods like pattern matching or hierarchical processing is involved. In this paper, we suggest an alternative method of providing target turnaround time and managing the priority of jobs while not doing any upfront resource modeling and resource planning. The methodology then systematically either meets the turnaround time need and potentially lets the user know if it will not as soon as possible. This builds on top of the Calibre Cluster Management (CalCM) resource management work previously published [1][2]. The paper describes the initial demonstration of the concept.

Flow visualization of lateral jet injection into swirling crossflow

NASA Technical Reports Server (NTRS)

Ferrell, G. B.; Aoki, K.; Lilley, D. G.

1985-01-01

Flow visualization experiments have been conducted to characterize the time-mean flowfield of a deflected turbulent jet in a confining cylindrical crossflow. Jet-to-crossflow velocity ratios of 2, 4, and 6 were investigated, under crossflow inlet swirler vane angles of 0 (swirler removed), 45 and 70 degrees. Smoke, neutrally-buoyant helium-filled soap bubbles, and multi-spark flow visualization were employed to highlight interesting features of the deflected jet, as well as the trajectory and spread pattern of the jet. Gross flowfield characterization was obtained for a range of lateral jet-to-crossflow velocity ratios and a range of inlet swirl strengths in the main flow. The flow visualization results agree well with the measurements obtained elsewhere with the six-orientation single hot-wire method.

Field Investigations of the July 2015 Pyroclastic Density Current Deposits of Volcán de Colima, Mexico

NASA Astrophysics Data System (ADS)

Atlas, Z. D.; Macorps, E.; Charbonnier, S. J.; Varley, N. R.

2016-12-01

Small-volume pyroclastic density currents (PDCs) occur relatively frequently and pose severe threats to surrounding populations and infrastructures at active explosive volcanoes. They are characterized by short duration and complex multiphase flow dynamics due to time and space variability in their properties, which include amongst others, particle concentration, granulometry, componentry, bulk rheology and velocity. Field investigations of the deposits emplaced by small-volume concentrated PDCs aim to improve our understanding of the transport and depositional processes of these flows: time and space variations in flow dynamics within a PDC moving downslope will reflect on the distribution, grainsize and component characteristics of its deposits. Our study focuses on the recent events of July 10th and 11th, 2015 at Volcán de Colima (Mexico) where the collapse of the recent lava dome complex and a portion of the southern crater rim led to the emplacement of successive pulses of small-volume concentrated PDCs on the southern flank, along the Montegrande and San Antonio ravines. A 3-dimensional field analysis of the PDCs' deposit architecture, total grain size distribution and component properties together with a geomorphic analysis of the affected ravines provide new insights on the lateral and vertical variations of flow dynamics for some of these small-volume concentrated PDCs. Preliminary results reveal three stratigraphic units with massive block, lapilli, ash facies within the valley confined and concentrated overbank deposits with increasing content in fines with distance from the summit, suggesting an increase in fragmentation processes within the PDCs. The middle unit is characterized by a finer grainsize, a higher accidental lithic content and a lower free crystal content. Moreover, direct correlations are found between rapid changes in channel morphology and generation of overbank (unconfined) flows that escaped valley confines, which could provide the basis for defining hazard zonations of key areas at risk from future eruptions at Colima.

Focusing and alignment of erythrocytes in a viscoelastic medium

NASA Astrophysics Data System (ADS)

Go, Taesik; Byeon, Hyeokjun; Lee, Sang Joon

2017-01-01

Viscoelastic fluid flow-induced cross-streamline migration has recently received considerable attention because this process provides simple focusing and alignment over a wide range of flow rates. The lateral migration of particles depends on the channel geometry and physicochemical properties of particles. In this study, digital in-line holographic microscopy (DIHM) is employed to investigate the lateral migration of human erythrocytes induced by viscoelastic fluid flow in a rectangular microchannel. DIHM provides 3D spatial distributions of particles and information on particle orientation in the microchannel. The elastic forces generated in the pressure-driven flows of a viscoelastic fluid push suspended particles away from the walls and enforce erythrocytes to have a fixed orientation. Blood cell deformability influences the lateral focusing and fixed orientation in the microchannel. Different from rigid spheres and hardened erythrocytes, deformable normal erythrocytes disperse from the channel center plane, as the flow rate increases. Furthermore, normal erythrocytes have a higher angle of inclination than hardened erythrocytes in the region near the side-walls of the channel. These results may guide the label-free diagnosis of hematological diseases caused by abnormal erythrocyte deformability.

Vertebrate Left-Right Asymmetry: What Can Nodal Cascade Gene Expression Patterns Tell Us?

PubMed Central

Schweickert, Axel; Ott, Tim; Kurz, Sabrina; Tingler, Melanie; Maerker, Markus; Fuhl, Franziska; Blum, Martin

2017-01-01

Laterality of inner organs is a wide-spread characteristic of vertebrates and beyond. It is ultimately controlled by the left-asymmetric activation of the Nodal signaling cascade in the lateral plate mesoderm of the neurula stage embryo, which results from a cilia-driven leftward flow of extracellular fluids at the left-right organizer. This scenario is widely accepted for laterality determination in wildtype specimens. Deviations from this norm come in different flavors. At the level of organ morphogenesis, laterality may be inverted (situs inversus) or non-concordant with respect to the main body axis (situs ambiguus or heterotaxia). At the level of Nodal cascade gene activation, expression may be inverted, bilaterally induced, or absent. In a given genetic situation, patterns may be randomized or predominantly lacking laterality (absence or bilateral activation). We propose that the distributions of patterns observed may be indicative of the underlying molecular defects, with randomizations being primarily caused by defects in the flow-generating ciliary set-up, and symmetrical patterns being the result of impaired flow sensing, on the left, the right, or both sides. This prediction, the reasoning of which is detailed in this review, pinpoints functions of genes whose role in laterality determination have remained obscure. PMID:29367579

Sample stream distortion modeled in continuous-flow electrophoresis

NASA Technical Reports Server (NTRS)

Rhodes, P. H.

1979-01-01

Buoyancy-induced disturbances in an electrophoresis-type chamber were investigated. Five tracer streams (latex) were used to visualize the flows while a nine-thermistor array sensed the temperature field. The internal heating to the chamber was provided by a 400 Hz electrical field. Cooling to the chamber was provided on the front and back faces and, in addition, on both chamber side walls. Disturbances to the symmetric base flow in the chamber occurred in the broad plane of the chamber and resulted from the formation of lateral and axial temperature gradients. The effect of these gradients was to retard or increase local flow velocities at different positions in the chamber cross section, which resulted in lateral secondary flows being induced in the broad plane of the chamber. As the adverse temperature gradients increased in magnitude, the critical Rayleigh number was approached and reverse (separated) flow became apparent, which, subsequently, led to the onset of time variant secondary flows.

Groundwater mixing and mineralization processes in a mountain-oasis-desert basin, northwest China: hydrogeochemistry and environmental tracer indicators

NASA Astrophysics Data System (ADS)

Ma, Bin; Jin, Menggui; Liang, Xing; Li, Jing

2018-02-01

Hydrogeochemistry and environmental tracers (2H, 18O, 87Sr/86Sr) in precipitation, river and reservoir water, and groundwater have been used to determine groundwater recharge sources, and to identify mixing characteristics and mineralization processes in the Manas River Basin (MRB), which is a typical mountain-oasis-desert ecosystem in arid northwest China. The oasis component is artificial (irrigation). Groundwater with enriched stable isotope content originates from local precipitation and surface-water leakage in the piedmont alluvial-oasis plain. Groundwater with more depleted isotopes in the north oasis plain and desert is recharged by lateral flow from the adjacent mountains, for which recharge is associated with high altitude and/or paleo-water infiltrating during a period of much colder climate. Little evaporation and isotope exchange between groundwater and rock and soil minerals occurred in the mountain, piedmont and oasis plain. Groundwater δ2H and δ18O values show more homogeneous values along the groundwater flow direction and with well depths, indicating inter-aquifer mixing processes. A regional contrast of groundwater allows the 87Sr/86Sr ratios and δ18O values to be useful in a combination with Cl, Na, Mg, Ca and Sr concentrations to distinguish the groundwater mixing characteristics. Two main processes are identified: groundwater lateral-flow mixing and river leakage in the piedmont alluvial-oasis plain, and vertical mixing in the north oasis plain and the desert. The 87Sr/86Sr ratios and selected ion ratios reveal that carbonate dissolution and mixing with silicate from the southern mountain area are primarily controlling the strontium isotope hydrogeochemistry.

Integrated Aero-Propulsion CFD Methodology for the Hyper-X Flight Experiment

NASA Technical Reports Server (NTRS)

Cockrell, Charles E., Jr.; Engelund, Walter C.; Bittner, Robert D.; Dilley, Arthur D.; Jentink, Tom N.; Frendi, Abdelkader

2000-01-01

Computational fluid dynamics (CFD) tools have been used extensively in the analysis and development of the X-43A Hyper-X Research Vehicle (HXRV). A significant element of this analysis is the prediction of integrated vehicle aero-propulsive performance, which includes an integration of aerodynamic and propulsion flow fields. This paper describes analysis tools used and the methodology for obtaining pre-flight predictions of longitudinal performance increments. The use of higher-fidelity methods to examine flow-field characteristics and scramjet flowpath component performance is also discussed. Limited comparisons with available ground test data are shown to illustrate the approach used to calibrate methods and assess solution accuracy. Inviscid calculations to evaluate lateral-directional stability characteristics are discussed. The methodology behind 3D tip-to-tail calculations is described and the impact of 3D exhaust plume expansion in the afterbody region is illustrated. Finally, future technology development needs in the area of hypersonic propulsion-airframe integration analysis are discussed.

Myosin1D is an evolutionarily conserved regulator of animal left-right asymmetry.

PubMed

Juan, Thomas; Géminard, Charles; Coutelis, Jean-Baptiste; Cerezo, Delphine; Polès, Sophie; Noselli, Stéphane; Fürthauer, Maximilian

2018-05-16

The establishment of left-right (LR) asymmetry is fundamental to animal development, but the identification of a unifying mechanism establishing laterality across different phyla has remained elusive. A cilia-driven, directional fluid flow is important for symmetry breaking in numerous vertebrates, including zebrafish. Alternatively, LR asymmetry can be established independently of cilia, notably through the intrinsic chirality of the acto-myosin cytoskeleton. Here, we show that Myosin1D (Myo1D), a previously identified regulator of Drosophila LR asymmetry, is essential for the formation and function of the zebrafish LR organizer (LRO), Kupffer's vesicle (KV). Myo1D controls the orientation of LRO cilia and interacts functionally with the planar cell polarity (PCP) pathway component VanGogh-like2 (Vangl2), to shape a productive LRO flow. Our findings identify Myo1D as an evolutionarily conserved regulator of animal LR asymmetry, and show that functional interactions between Myo1D and PCP are central to the establishment of animal LR asymmetry.

Magnetic Control of Lateral Migration of Ellipsoidal Microparticles in Microscale Flows

NASA Astrophysics Data System (ADS)

Zhou, Ran; Sobecki, Christopher A.; Zhang, Jie; Zhang, Yanzhi; Wang, Cheng

2017-08-01

Precise manipulations of nonspherical microparticles by shape have diverse applications in biology and biomedical engineering. Here, we study lateral migration of ellipsoidal paramagnetic microparticles in low-Reynolds-number flows under uniform magnetic fields. We show that magnetically induced torque alters the rotation dynamics of the particle and results in shape-dependent lateral migration. By adjusting the direction of the magnetic field, we demonstrate versatile control of the symmetric and asymmetric rotation of the particles, thereby controlling the direction of the particle's lateral migration. The particle rotations are experimentally measured, and their symmetry or asymmetry characteristics agree well with the prediction from a simple theory. The lateral migration mechanism is found to be valid for nonmagnetic particles suspended in a ferrofluid. Finally, we demonstrate shape-based sorting of microparticles by exploiting the proposed migration mechanism.

Salix response to different flow regimes in controlled experiments: first results

NASA Astrophysics Data System (ADS)

Gorla, Lorenzo; Signarbieux, Constant; Buttler, Alexandre; Perona, Paolo

2013-04-01

Dams and water management for hydropower production, agriculture and other human activities alter the natural flow regime of rivers. The new river hydrograph components depend on the type of impoundment and the policy of regulation but such a different flow regime will likely affect the riparian environment. The main challenge in order to define sustainable flow releases is to quantify hydrological effects in terms of geomorphology and ecosystem response. A considerable lack of knowledge still affects the link hydrology-ecology and inadequate flow rules (e.g., minimal or residual flows) are consequently still widespread: further research in this direction is urgently required. We present an experiment, which aims to investigate the effects of different water stage regimes on riparian vegetation (salix Viminalis cuttings) development in a temperate region (Switzerland). This work describes the installation setup, together with the first results concerning the first of the two scheduled seasons of campaign. Sixty Salix cuttings were planted in non-cohesive sandy-gravel sediment within 1 meter tall plastic pots installed outside in the EPFL campus. After grouping them in three batteries, the water level within them has been varying following three river regimes simulated by adjusting the water level within the pots by means of an automatic hydraulic system. The three water level regimes reproduce a natural flow regime, a minimum residual flow policy, which only conserves peaks during flooding conditions, and an artificial regime conserving only low frequencies (e.g., seasonality) of the natural dynamic. The natural flow regime of the first battery has been applied for two months to the entire system; the three regimes above said started in June 2012. This triggered a plant response transitory regime, which we monitored by measuring plant growth, soil and atmospheric variables. Particularly, measures concern with branches development leaves photosynthesis and fluorescence, together with pictures of each plant. Sap flow was measured for thirty cuttings using a time resolution of thirty minutes, whereas psychrometers measuring the water potential were sampling data every fifteen minutes. Soil moisture and meteo data have also been collected as essential drivers of plant response: these data as well as sap flow measurements can be later compared to a similar field installation along Thur River (Switzerland). After the first season of measurement, in 2012, part of the cuttings have been carefully removed and further analyzed as far as the below ground biomass is concerned. Strong differences in terms of stress and growth performances were observed in correspondence of the transitional phase, following the alterations of the natural flow regime. A later adjustment in the roots distribution allowed survivors to re-sprout and to withstand new conditions.

Quantal mEPSCs and residual glutamate: how horizontal cell responses are shaped at the photoreceptor ribbon synapse

PubMed Central

Cadetti, Lucia; Bartoletti, Theodore M.; Thoreson, Wallace B.

2012-01-01

At the photoreceptor ribbon synapse, glutamate released from vesicles at different positions along the ribbon reaches the same postsynaptic receptors. Thus, vesicles may not exert entirely independent effects. We examined whether responses of salamander retinal horizontal cells evoked by light or direct depolarization during paired recordings could be predicted by summation of individual miniature excitatory postsynaptic currents (mEPSCs). For EPSCs evoked by depolarization of rods or cones, linear convolution of mEPSCs with photoreceptor release functions predicted EPSC waveforms and changes caused by inhibiting glutamate receptor desensitization. A low-affinity glutamate antagonist, kynurenic acid (KynA), preferentially reduced later components of rod-driven EPSCs, suggesting lower levels of glutamate are present during the later sustained component of the EPSC. A glutamate-scavenging enzyme, glutamic-pyruvic transaminase, did not inhibit mEPSCs or the initial component of rod-driven EPSCs, but reduced later components of the EPSC. Inhibiting glutamate uptake with a low concentration of dl-threo-β-benzoyloxyaspartate (TBOA) also did not alter mEPSCs or the initial component of rod-driven EPSCs, but enhanced later components of the EPSC. Low concentrations of TBOA and KynA did not affect the kinetics of fast cone-driven EPSCs. Under both rod- and cone-dominated conditions, light-evoked currents (LECs) were enhanced considerably by TBOA. LECs were more strongly inhibited than EPSCs by KynA, suggesting the presence of lower glutamate levels. Collectively, these results indicate that the initial EPSC component can be largely predicted from a linear sum of individual mEPSCs, but with sustained release, residual amounts of glutamate from multiple vesicles pool together, influencing LECs and later components of EPSCs. PMID:18547244

Upper Mantle Texture Patterns In Eastern North America From Seismic Anisotropy And Global Mantle Flow Calculations

NASA Astrophysics Data System (ADS)

Levin, V. L.; Moucha, R.; Yuan, H.

2013-12-01

Global seismic models show gradual and systematic changes in upper mantle seismic properties beneath North America. Faster and thicker lithosphere of the interior thins eastward. Upper mantle rock fabric reflected in observations of seismic anisotropy also varies. Near the coast apparent fast directions of split shear waves are nearly east-west, with considerable scatter. Further inland they are more uniform and align SW-NE, close to the absolute plate motion direction of North America. Mantle convection simulations driven by density inferred from global joint seismic-geodynamic tomography models exhibit complex flow beneath the eastern edge of the North American continent due to the ongoing descent of the Farallon slab deep beneath it (figure 1). Flow predicted beneath the coast is nearly horizontal with a small, though dynamically important, vertical component, while west of the Appalachians it turns downward. Long records of teleseismic observations accumulated at permanent seismic stations HRV, PAL and SSPA (figure 2) are inverted for vertical distribution of anisotropic parameters. We find preference for more than one layer of anisotropy beneath all sites, with significantly different parameters that could reflect either lateral variations in the lithospheric thickness, variations in the asthenospheric flow field, or both. Since we find considerable consistency in directional patterns of P-to-S mode converted waves associated with the lower part of the lithosphere, variations of asthenospheric flow seem to be a more plausible explanation. We explore the links between predicted flow and inferences from seismic data with additional observations of anisotropy and calculations of flow-induced rock fabric.

Geology of proximal, small-volume trachyte-trachyandesite pyroclastic flows and associated surge deposits, Roccamonfina volcano, Italy

NASA Astrophysics Data System (ADS)

Giannetti, Bernardino

1998-01-01

This paper describes the 232 ka B.P. MTTT trachyte-trachyandesite pyroclastic succession of Roccamonfina volcano. This small-volume, proximal sequence crops out along Mulino di Sotto, Paratone, and Pisciariello ravines in the southwest sector of the central caldera, and covers a minimum extent of 3.5 km 2 area. It is made up of seven pyroclastic flows and pyroclastic surge units consisting of trachytic ash matrix containing juvenile trachyandesitic scoria and dense lava fragments, pumice clasts of uncertain trachyandesite, and a foreign trachyandesitic lithic facies. Two stratigraphic markers allow correlation of the units. No paleosoils and Plinian fallout have been observed at the base and within the succession. Some lateral grading of scoria and lithic clasts suggests that MTTT derived from three distinct source vents. The sequence consists of a basal ash flow passing laterally to laminated surge deposits (Unit A). This is overlain by a reversely graded scoria and pumice lapilli flow (Unit B) which is in turn overlain by a thinly cross-stratified scoria lapilli surge (Unit C). Unit C is capped by a prominent ash-and-scoria flow (Unit D). A ground layer (Marker MK1) divides Unit D from a massive ignimbrite which grades upcurrent to sand-wave surge deposits (Unit E). Another ground layer (Marker MK2) separates Unit E from Unit F. This unit consists of a basal ignimbrite passing laterally to bedded surge deposits with convolute structures (subunit Fl), and grading upcurrent to a subhorizontally plane-laminated ash cloud (subunit F2) containing near the top a layer of millimetric lithic clasts embedded in fine ash. The succession is closed by the pyroclastic flow Unit G. Surge Unit C can be interpreted in terms of vertical gradients in turbulence, particle concentration, and velocity during flowage, whereas the bedded surge parts present in the massive deposits of Units A and E-F1 can be related to abrupt changes of velocity down the steep slopes of ravines. Reverse grading in Unit B is probably due to grain dispersive pressures. The convolute structures within Fl are related to zones of diagenetic cementation associated with groundwater. Finally, the laminated, fine-grained nature of subunit F2 is interpreted as due to ash clouds elutriated from the basal part of Unit F. Stratigraphic markers MK1-MK2 are ground layer breccias formed by settling of lithic and scoria clasts from overlying units E and F, respectively. Vesiculation and morphologies of glass shards of the MTTT succession suggest that eruptions were essentially driven by magmatic explosions which had an appreciable hydromagmatic component.

Lateral Migration and Rotational Motion of Elliptic Particles in Planar Poiseuille Flow

NASA Technical Reports Server (NTRS)

Qi, Dewei; Luo, Li-Shi; Aravamuthan, Raja; Strieder, William; Bushnell, Dennis M. (Technical Monitor)

2002-01-01

Simulations of elliptic particulate suspensions in the planar Poiseuille flow are performed by using the lattice Boltzmann equation. Effects of the multi-particle on the lateral migration and rotational motion of both neutrally and non-neutrally buoyant elliptic particles are investigated. Low and intermediate total particle volume fraction f(sub a) = 13%, 15%, and 40% are considered in this work.

Goldfish and oscars have comparable responsiveness to dipole stimuli

NASA Astrophysics Data System (ADS)

Nauroth, Ines Eva; Mogdans, Joachim

2009-12-01

The relative roles of the fish lateral line and inner ear for the perception of hydrodynamic stimuli are poorly investigated. Here, we studied responsiveness to a 100 Hz vibrating sphere (dipole stimulus) of goldfish and oscars, two species that differ in peripheral lateral line morphology, inner ear morphology, mechanical linkage between inner ear and swim bladder, and inner ear sensitivity. We measured unconditioned dipole-evoked changes in breathing activity in still water and in the presence of a 5-cm s-1 background flow. In still water, individuals from both species responded to sound pressure levels (SPLs) between 92 and 109 dB SPL re 1 μPaRMS. Responsiveness was not affected by background flow or by temporary inactivation of the lateral line. The data suggest that fish with different lateral line and inner ear morphologies have similar sensitivities to vibrating sphere stimuli and can detect and respond to dipole sources equally well in still water and in moderate background flows. Moreover, behavioral responses were not dependent on a functional lateral line, suggesting that in this type of experiment, the inner ear is the dominant sense organ for the perception of hydrodynamic stimuli.

Effects of Structural Flexibility on Motorcycle Straight Running Stability by using Energy Flow Method

NASA Astrophysics Data System (ADS)

Marumo, Yoshitaka; Katayama, Tsuyoshi

This study uses the energy flow method to analyze how structural flexibility affects the motorcycle wobble and weave modes. Lateral bending of the front fork and torsion of the main frame affect the wobble mode stability. These are based on the gyroscopic effect of the front wheel in the steering motion by considering structural flexibility. At high speeds, lateral bending of the front fork and torsion of the rear swing arm more significantly affect the weave mode stability. These are primarily due to the phase changes of the external force generated by the yaw rate in the lateral motion. The phase change of the yaw rate force in the lateral motion originates from the phase change of the tire side forces.

A bio-inspired real-time capable artificial lateral line system for freestream flow measurements.

PubMed

Abels, C; Qualtieri, A; De Vittorio, M; Megill, W M; Rizzi, F

2016-06-03

To enhance today's artificial flow sensing capabilities in aerial and underwater robotics, future robots could be equipped with a large number of miniaturized sensors distributed over the surface to provide high resolution measurement of the surrounding fluid flow. In this work we show a linear array of closely separated bio-inspired micro-electro-mechanical flow sensors whose sensing mechanism is based on a piezoresistive strain-gauge along a stress-driven cantilever beam, mimicking the biological superficial neuromasts found in the lateral line organ of fishes. Aiming to improve state-of-the-art flow sensing capability in autonomously flying and swimming robots, our artificial lateral line system was designed and developed to feature multi-parameter freestream flow measurements which provide information about (1) local flow velocities as measured by the signal amplitudes from the individual cantilevers as well as (2) propagation velocity, (3) linear forward/backward direction along the cantilever beam orientation and (4) periodicity of pulses or pulse trains determined by cross-correlating sensor signals. A real-time capable cross-correlation procedure was developed which makes it possible to extract freestream flow direction and velocity information from flow fluctuations. The computed flow velocities deviate from a commercial system by 0.09 m s(-1) at 0.5 m s(-1) and 0.15 m s(-1) at 1.0 m s(-1) flow velocity for a sampling rate of 240 Hz and a sensor distance of 38 mm. Although experiments were performed in air, the presented flow sensing system can be applied to underwater vehicles as well, once the sensors are embedded in a waterproof micro-electro-mechanical systems package.

Formation and evolution of a metasomatized lithospheric root at the motionless Antarctic plate: the case of East Island, Crozet Archipelago (Indian Ocean)

NASA Astrophysics Data System (ADS)

Meyzen, Christine; Marzoli, Andrea; Bellieni, Giuliano; Levresse, Gilles

2016-04-01

Sitting atop the nearly stagnant Antarctic plate (ca. 6.46 mm/yr), the Crozet archipelago midway between Madagascar and Antarctica constitutes a region of unusually shallow (1543-1756 m below sea level) and thickened oceanic crust (10-16.5 km), high geoid height, and deep low-velocity zone, which may reflect the surface expression of a mantle plume. Here, we present new major and trace element data for Quaternary sub-aerial alkali basalts from East Island, the easterly and oldest island (ca. 9 Ma) of the Crozet archipelago. Crystallization at uppermost mantle depth and phenocryst accumulation have strongly affected their parental magma compositions. Their trace element patterns show a large negative K anomaly relative to Ta-La, moderate depletions in Rb and Ba with respect to Th-U, and heavy rare earth element (HREE) depletions relative to light REE. These characteristics allow limits to be placed upon the composition and mineralogy of their mantle source. The average trace element spectrum of East Island basalts can be matched by melting of about 2 % of a garnet-phlogopite-bearing peridotite source. The stability field of phlogopite restricts melting depth to lithospheric levels. The modelled source composition requires a multistage evolution, where the mantle has been depleted by melt extraction before having been metasomatized by alkali-rich plume melts. The depleted mantle component may be sourced by residual mantle plume remnants stagnated at the melting locus due to a weak lateral flow velocity inside the melting regime, whose accumulation progressively edifies a depleted lithospheric root above the plume core. Low-degree alkali-rich melts are likely derived from the plume source. Such a mantle source evolution may be general to both terrestrial and extraterrestrial environments where the lateral component velocity of the mantle flow field is extremely slow.

Modelling river bank retreat by combining fluvial erosion, seepage and mass failure

NASA Astrophysics Data System (ADS)

Dapporto, S.; Rinaldi, M.

2003-04-01

Streambank erosion processes contribute significantly to the sediment yielded from a river system and represent an important issue in the contexts of soil degradation and river management. Bank retreat is controlled by a complex interaction of hydrologic, geotechnical, and hydraulic processes. The capability of modelling these different components allows for a full reconstruction and comprehension of the causes and rates of bank erosion. River bank retreat during a single flow event has been modelled by combining simulation of fluvial erosion, seepage, and mass failures. The study site, along the Sieve River (Central Italy), has been subject to extensive researches, including monitoring of pore water pressures for a period of 4 years. The simulation reconstructs fairly faithfully the observed changes, and is used to: a) test the potentiality and discuss advantages and limitations of such type of methodology for modelling bank retreat; c) quantify the contribution and mutual role of the different processes determining bank retreat. The hydrograph of the event is divided in a series of time steps. Modelling of the riverbank retreat includes for each step the following components: a) fluvial erosion and consequent changes in bank geometry; b) finite element seepage analysis; c) stability analysis by limit equilibrium method. Direct fluvial shear erosion is computed using empirically derived relationships expressing lateral erosion rate as a function of the excess of shear stress to the critical entrainment value for the different materials along the bank profile. Lateral erosion rate has been calibrated on the basis of the total bank retreat measured by digital terrestrial photogrammetry. Finite element seepage analysis is then conducted to reconstruct the saturated and unsaturated flow within the bank and the pore water pressure distribution for each time step. The safety factor for mass failures is then computed, using the pore water pressure distribution obtained by the seepage analysis, and the geometry of the upper bank is modified in case of failure.

VORSTAB: A computer program for calculating lateral-directional stability derivatives with vortex flow effect

NASA Technical Reports Server (NTRS)

Lan, C. Edward

1985-01-01

A computer program based on the Quasi-Vortex-Lattice Method of Lan is presented for calculating longitudinal and lateral-directional aerodynamic characteristics of nonplanar wing-body combination. The method is based on the assumption of inviscid subsonic flow. Both attached and vortex-separated flows are treated. For the vortex-separated flow, the calculation is based on the method of suction analogy. The effect of vortex breakdown is accounted for by an empirical method. A summary of the theoretical method, program capabilities, input format, output variables and program job control set-up are described. Three test cases are presented as guides for potential users of the code.

Economic evaluation of laboratory testing strategies for hospital-associated Clostridium difficile infection.

PubMed

Schroeder, Lee F; Robilotti, Elizabeth; Peterson, Lance R; Banaei, Niaz; Dowdy, David W

2014-02-01

Clostridium difficile infection (CDI) is the most common cause of infectious diarrhea in health care settings, and for patients presumed to have CDI, their isolation while awaiting laboratory results is costly. Newer rapid tests for CDI may reduce this burden, but the economic consequences of different testing algorithms remain unexplored. We used decision analysis from the hospital perspective to compare multiple CDI testing algorithms for adult inpatients with suspected CDI, assuming patient management according to laboratory results. CDI testing strategies included combinations of on-demand PCR (odPCR), batch PCR, lateral-flow diagnostics, plate-reader enzyme immunoassay, and direct tissue culture cytotoxicity. In the reference scenario, algorithms incorporating rapid testing were cost-effective relative to nonrapid algorithms. For every 10,000 symptomatic adults, relative to a strategy of treating nobody, lateral-flow glutamate dehydrogenase (GDH)/odPCR generated 831 true-positive results and cost $1,600 per additional true-positive case treated. Stand-alone odPCR was more effective and more expensive, identifying 174 additional true-positive cases at $6,900 per additional case treated. All other testing strategies were dominated by (i.e., more costly and less effective than) stand-alone odPCR or odPCR preceded by lateral-flow screening. A cost-benefit analysis (including estimated costs of missed cases) favored stand-alone odPCR in most settings but favored odPCR preceded by lateral-flow testing if a missed CDI case resulted in less than $5,000 of extended hospital stay costs and <2 transmissions, if lateral-flow GDH diagnostic sensitivity was >93%, or if the symptomatic carrier proportion among the toxigenic culture-positive cases was >80%. These results can aid guideline developers and laboratory directors who are considering rapid testing algorithms for diagnosing CDI.

Proteomic Identification of Immunodiagnostic Antigens for Trypanosoma vivax Infections in Cattle and Generation of a Proof-of-Concept Lateral Flow Test Diagnostic Device.

PubMed

Fleming, Jennifer R; Sastry, Lalitha; Wall, Steven J; Sullivan, Lauren; Ferguson, Michael A J

2016-09-01

Trypanosoma vivax is one of the causative agents of Animal African Trypanosomosis in cattle, which is endemic in sub-Saharan Africa and transmitted primarily by the bite of the tsetse fly vector. The parasite can also be mechanically transmitted, and this has allowed its spread to South America. Diagnostics are limited for this parasite and in farm settings diagnosis is mainly symptom-based. We set out to identify, using a proteomic approach, candidate diagnostic antigens to develop into an easy to use pen-side lateral flow test device. Two related members the invariant surface glycoprotein family, TvY486_0045500 and TvY486_0019690, were selected. Segments of these antigens, lacking N-terminal signal peptides and C-terminal transmembrane domains, were expressed in E. coli. Both were developed into ELISA tests and one of them, TvY486_0045500, was developed into a lateral flow test prototype. The tests were all evaluated blind with 113 randomised serum samples, taken from 37 calves before and after infection with T. vivax or T. congolense. The TvY486_0045500 and TvY486_0019690 ELISA tests gave identical sensitivity and specificity values for T. vivax infection of 94.5% (95% CI, 86.5% to 98.5%) and 88.0% (95% CI, 75.7% to 95.5%), respectively, and the TvY486_0045500 lateral flow test prototype a sensitivity and specificity of 92.0% (95% CI, 83.4% to 97.0%) and 89.8% (95% CI, 77.8% to 96.6%), respectively. These data suggest that recombinant TvY486_0045500 shows promise for the development of a pen-side lateral flow test for the diagnosis of T. vivax animal African trypanosomosis.

Economic Evaluation of Laboratory Testing Strategies for Hospital-Associated Clostridium difficile Infection

PubMed Central

Robilotti, Elizabeth; Peterson, Lance R.; Banaei, Niaz; Dowdy, David W.

2014-01-01

Clostridium difficile infection (CDI) is the most common cause of infectious diarrhea in health care settings, and for patients presumed to have CDI, their isolation while awaiting laboratory results is costly. Newer rapid tests for CDI may reduce this burden, but the economic consequences of different testing algorithms remain unexplored. We used decision analysis from the hospital perspective to compare multiple CDI testing algorithms for adult inpatients with suspected CDI, assuming patient management according to laboratory results. CDI testing strategies included combinations of on-demand PCR (odPCR), batch PCR, lateral-flow diagnostics, plate-reader enzyme immunoassay, and direct tissue culture cytotoxicity. In the reference scenario, algorithms incorporating rapid testing were cost-effective relative to nonrapid algorithms. For every 10,000 symptomatic adults, relative to a strategy of treating nobody, lateral-flow glutamate dehydrogenase (GDH)/odPCR generated 831 true-positive results and cost $1,600 per additional true-positive case treated. Stand-alone odPCR was more effective and more expensive, identifying 174 additional true-positive cases at $6,900 per additional case treated. All other testing strategies were dominated by (i.e., more costly and less effective than) stand-alone odPCR or odPCR preceded by lateral-flow screening. A cost-benefit analysis (including estimated costs of missed cases) favored stand-alone odPCR in most settings but favored odPCR preceded by lateral-flow testing if a missed CDI case resulted in less than $5,000 of extended hospital stay costs and <2 transmissions, if lateral-flow GDH diagnostic sensitivity was >93%, or if the symptomatic carrier proportion among the toxigenic culture-positive cases was >80%. These results can aid guideline developers and laboratory directors who are considering rapid testing algorithms for diagnosing CDI. PMID:24478478

A uniform method for the simultaneous blood group phenotyping of Fya , Fyb , Jka , Jkb , S, s̅, P1, k applying lateral-flow technique.

PubMed

Caesar, A; Meyer, S; Trost, N; Neuenschwander, K; Geisen, C; Frey, B M; Gassner, C; Schwind, P

2018-02-01

A lateral flow assay for simultaneous blood group typing of ABO, RhD, C, E, c, e, Cw and K with stable end-point and without centrifugation is in routine use since several years (MDmulticard ® ). The typing of extended phenotype parameters belonging to the Duffy, Kidd, MNSs blood group systems and others, however, has not yet been demonstrated for this technique. Reliable detection of Fy x , a weak Fy b phenotype with a pronounced quantitative reduction of the number of Fy b antigens on the erythrocyte surface, remains a weakness of current serological blood grouping techniques. The performance characteristics of the following reagents were evaluated in donor and patient samples in lateral flow technology (MDmulticard ® ): Anti-Fy a , -Fy b , -Jk a , -Jk b , -S, -s̅, -P1 and -k. The sensitivity to detect Fy x was in addition evaluated with Fy x positive samples, which had been preselected by MALDI-TOF MS-based genotyping. All results obtained with the MDmulticard ® were in full accordance with those of the CE-certified reference products for all the eight reagent formulations used: Anti-Fy a , -Fy b , -Jk a , -Jk b , -S, -s̅, -P1 and -k. Also, all Fy x phenotypes of the selected population of 93 positive samples, originally identified by MALDI-TOF MS-based genotyping, were reliably detected by the lateral flow assay. Extended phenotype blood group parameters, including the serologically challenging Fy x phenotype, can be determined simultaneously, rapidly and accurately using the lateral flow (MDmulticard ® ) technology, even in cases when IgG class antibodies are the only source of diagnostic antibodies. © 2017 International Society of Blood Transfusion.

Microfabrication of IPMC cilia for bio-inspired flow sensing

NASA Astrophysics Data System (ADS)

Lei, Hong; Li, Wen; Tan, Xiaobo

2012-04-01

As the primary flow sensing organ for fishes, the lateral line system plays a critical role in fish behavior. Analogous to its biological counterpart, an artificial lateral line system, consisting of arrays of micro flow sensors, is expected to be instrumental in the navigation and control of underwater robots. In this paper we investigate the microfabrication of ionic polymer-metal composite (IPMC) cilia for the purpose of flow sensing. While existing macro- and microfabrication methods for IPMCs have predominantly focused on planar structures, we propose a device where micro IPMC beams stand upright on a substrate to effectively interact with the flow. Challenges in the casting of 3D Nafion structure and selective formation of electrodes are discussed, and potential solutions for addressing these challenges are presented together with preliminary microfabrication results.

Incompressible Turbulent Wing-Body Junction Flow

NASA Technical Reports Server (NTRS)

Krishnamurthy, R.; Cagle, Corey D.; Chandra, S.

1998-01-01

The overall objective of this study is to contribute to the optimized design of fan bypass systems in advanced turbofan engines. Increasing the engine bypass ratios have provided a major boost in engine performance improvement over the last fifty years. An engine with high bypass ratio (11-16:1) such as the Advanced Ducted Propulsion (ADP) is being developed and is expected to provide an additional 25% improvement in overall efficiency over the early turbofans. Such significant improvements in overall efficiency would reduce the cost per seat mile, which is a major government and Industry challenge for the 21th century. The research is part of the Advanced Subsonic Technology (AST) program that involves a NASA, U.S. Industry and FAA partnership with the goal of a safe and highly productive global air transportation system. The immediate objective of the study is to perform numerical simulation of duct-strut interactions to elucidate the loss mechanisms associated with this configuration that is typical of advanced turbofan engines such as ADP. However, at present experimental data for a duct-strut configuration are not available. Thus, as a first step a wing-body junction flow would be studied and is the specific objective of the present study. At the outset it is to be recognized that while duct-strut interaction flow is similar to that of wing-body junction flows, there are some differences owing to the presence of a wall at both ends of the strut. Likewise, some differences are due to the sheared inflow (as opposed to a uniform inflow) velocity profile. It is however expected that some features of a wing-body junction flow would persist. Next, some of the salient aspects of the complex flow near a wing-body junction, as revealed by various studies reported in the literature will be reviewed. One of the principle characteristics of the juncture flow, is the presence of the mean flow components in a plane perpendicular to the direction of the oncoming free-stream flow. The lateral curvature of the wing/strat causes the oncoming turbulent layer to skew about am axis (x-axis) parallel to the plane (xz-plane) of the mean shear. This is the principle mechanism for the generation of secondary flow. Such skew-induced secondary flows are slow to be attenuated by Reynolds stresses. Additional contribution to the generation of secondary flow comes from anisotropies in Reynolds stresses. Upstream of the strut, the mean-vorticity is directed span wise (along the y-direction). The presence of secondary flow in the vicinity of the strut causes the vorticity to stretch around the obstacle in a horse-shoe shape, with each leg having a vorticity of the opposite sense. The blockage effect of the strut imposes a severe adverse pressure gradient on the oncoming turbulent shear layer, causing boundary layer separation ahead of the leading edge, resulting in a vortex that rolls up and flows downstream into the juncture region. The separation vortices trailing in the wake of the wing can alter the lift or drag characteristics of the surfaces downstream of the wing-body juncture. Likewise, on submarines, the wake flow behind the appendage can degrade the performance of the propeller located downstream. The complex nature of this flow is caused by the presence of all six components of Reynolds stresses. Devenport and Simpson report that in the vicinity of the horse-shoe vortex there is intense recirculation with turbulent stresses being much larger than those normally observed in turbulent flows. These features contribute to making this flow a challenge to predict numerically. Some of the past studies provide useful insights into this flow that would guide our numerical efforts. In measurements reported by Shabaka and Bradshaw, the eddy viscosity tensor is seen to be non-isotropic and has negative components in certain regions. In an effort to evaluate the closure assumptions of various turbulence models, Devenport and Simpson used their own extensive measurements in juncture flows around the nose of a wing-body junction. Measured values of mean-velocity and/or turbulence kinetic energy was used to predict the magnitude of the shear stress vector. Algebraic stress models performed the best followed by Cebeci-Smith eddy viscosity model. The flow is reported to be dominated by a pressure field produced by the wing and the velocity field generated by the horseshoe vortex that is wrapped around the junction between the wing and wall. Kubendran et al. conclude from an experimental study that the shape of leading edge of the wing as characterized by its slenderness ratio is a major factor in determining the flow fields in the juncture region. The more thinner the leading edge of the juncture, the weaker the horseshoe vortex is. Also, with a slender leading edge, the secondary flow in the juncture would be due mainly to the cross-stream gradients of Reynolds stresses rather than due to a lateral skewing of the shear layer.

[Thyroid echogeneity as a useful tool for the differential diagnosis of hyperthyroidism in the course of Graves disease and Hashimoto thyroiditis].

PubMed

Niedziela, M; Warzywoda, M; Korman, E

2000-01-01

Hashimoto's thyroiditis (HT) and Graves' disease (GD) constitute a spectrum of autoimmune thyroid diseases (AITD). They share an autoimmune pathogenesis, with a cellular and a humoral response to the thyroid gland. As a consequence, dysfunction of the gland itself may develop, characterized by hyperfunction in the case of GD and hypofunction in the case of HT, however at the onset of HT the hyperthyroidism might be observed as a result of a rapid destruction of thyrocytes. An abnormal thyroid echographic pattern characterized by a diffuse low echogeneity has been described in both AITD. This hypoechogeneity is due to three components: increase of intrathyroidal flow, functional changes in thyroid follicles with increased cellularity and decrease of the colloid content, resulting in the reduction of the cell/colloid interface, variable degree of lymphocytic infiltration. The first two components may be reversible during medical treatment and seem to be characteristic for GD, whereas lymphocytic infiltration may rather represent mostly HT. Here we present a 17-year-old girl with typical clinical signs of hyperthyroidism [firm goiter (II degrees), tachycardia, palpitations, nervousness, excessive sweating and tremor]. Laboratory tests were the following: fT3 - 6.59 pg/ml(increasing), fT4 - 1.99 ng/dl(increasing), TSH - 0.02 micro IU/ml(decreasing); anti-Tg-Ab - 840 IU/ml(increasing), anti-TPO-Ab - 190 IU/ml(increasing) (4 months later antithyroid antibodies were 2200 and 70, respectively). Ultrasound examination showed hypoechogeneity of the whole gland and enhanced vascular flow based on power Doppler analysis. Thyroid scan visualized the generally increased uptake of technetium. The girl was put on beta-blocker (propranolol) and later an antithyroid drug (thiamazole) was added. A course of disease was unstable, therefore the fine-needle aspiration biopsy was performed and showed the presence of single groups of normal thyrocytes and scanty colloid with no features of HT. Power Doppler analysis showed still enhanced blood flow within a gland inspite of euthyroid state. After a very unsteady period of the disease, the euthyroid state is maintained although the medical treatment was given up. The full recovery of normal blood flow and normal echogeneity of the thyroid was documented. The latter supports the diagnosis of GD. Follow-up of the thyroid echogeneity is of great diagnostic and prognostic value if the assay of TSHR-Ab is not available. On the other side, it has to be remembered that TSHR-Ab do not have to be positive in patients with GD and can be positive in patients with HT.

Study of the flow mixing in a novel ARID raceway for algae production

DOE PAGES

Xu, Ben; Li, Peiwen; Waller, P.

2014-07-31

A novel flow field for algae raceways has been proposed, which is fundamentally different from traditional paddlewheel-driven raceways. To reduce freezing and heat loss in the raceway during cold time, the water is drained to a deep storage canal. The ground bed of the new raceway has a low slope so that water, lifted by propeller pump, can flow down in laterally-laid serpentine channels, relying on gravitational force. The flow rate of water is controlled so that it can overflow the lateral channel walls and mix with the main flow in the next lower channel, which thus creates a bettermore » mixing. In order to optimize the design parameters of the new flow field, methods including flow visualization, local point velocity measurement, and CFD analysis were employed to investigate the flow mixing features. Different combinations of channel geometries and water velocities were evaluated. An optimized flow field design and details of flow mixing are presented. The study offers an innovative design for large scale algae growth raceways which is of significance to the algae and biofuel industry.« less

Study of the flow mixing in a novel ARID raceway for algae production

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xu, Ben; Li, Peiwen; Waller, P.

A novel flow field for algae raceways has been proposed, which is fundamentally different from traditional paddlewheel-driven raceways. To reduce freezing and heat loss in the raceway during cold time, the water is drained to a deep storage canal. The ground bed of the new raceway has a low slope so that water, lifted by propeller pump, can flow down in laterally-laid serpentine channels, relying on gravitational force. The flow rate of water is controlled so that it can overflow the lateral channel walls and mix with the main flow in the next lower channel, which thus creates a bettermore » mixing. In order to optimize the design parameters of the new flow field, methods including flow visualization, local point velocity measurement, and CFD analysis were employed to investigate the flow mixing features. Different combinations of channel geometries and water velocities were evaluated. An optimized flow field design and details of flow mixing are presented. The study offers an innovative design for large scale algae growth raceways which is of significance to the algae and biofuel industry.« less

Hydrodynamic simulations of pulsar glitch recovery

NASA Astrophysics Data System (ADS)

Howitt, G.; Haskell, B.; Melatos, A.

2016-08-01

Glitches are sudden jumps in the spin frequency of pulsars believed to originate in the superfluid interior of neutron stars. Superfluid flow in a model neutron star is simulated by solving the equations of motion of a two-component superfluid consisting of a viscous proton-electron plasma and an inviscid neutron condensate in a spherical Couette geometry. We examine the response of the model to glitches induced in three different ways: by instantaneous changes of the spin frequency of the inner and outer boundaries, and by instantaneous recoupling of the fluid components in the bulk. All simulations are performed with strong and weak mutual friction. It is found that the maximum size of a glitch originating in the bulk decreases as the mutual friction strengthens. It is also found that mutual friction determines the fraction of the frequency jump which is later recovered, a quantity known as the `healing parameter'. These behaviours may explain some of the diversity in observed glitch recoveries.

Comparison of intraoperative anthropometric measurements of the proximal tibia and tibial component in total knee arthroplasty.

PubMed

Miyatake, Naohisa; Sugita, Takehiko; Aizawa, Toshimi; Sasaki, Akira; Maeda, Ikuo; Kamimura, Masayuki; Fujisawa, Hirokazu; Takahashi, Atsushi

2016-09-01

Precise matching of the tibial component and resected bony surfaces and proper rotational implanting of the tibial component are crucial for successful total knee arthroplasty. We aimed to analyze the exact anthropometric proximal tibial data of Japanese patients undergoing total knee arthroplasty and correlate the measurements with the dimensions of current total knee arthroplasty systems. A total of 703 knees in 566 Japanese patients who underwent total knee arthroplasty for osteoarthritis were included. The bone resection in the proximal tibia was performed perpendicular to the tibial axis in the frontal plane. Measurements of the proximal tibia were intraoperatively obtained after proximal tibial preparation. There were significant positive correlations between the lateral anteroposterior and medial anteroposterior and mediolateral dimensions. A progressive decrease in the mediolateral/lateral anteroposterior ratio with an increasing lateral anteroposterior dimension or the mediolateral/anteroposterior ratio with an increasing anteroposterior dimension was observed. The lateral anteroposterior dimension was smaller than the medial anteroposterior dimension by a mean of 4.8 ± 2.0 mm. The proximal tibia exhibited asymmetry between the lateral and medial plateaus. A comparison of the morphological data and dimensions of the implants, one of which was a symmetric tibial component (NexGen) and the others were asymmetric (Genesis II and Persona), indicated that an asymmetric tibial component could be beneficial to maximize tibial plateau coverage. This study provided important reference data for designing a proper tibial component for Japanese people. The proximal tibial cut surface was asymmetric. There was wide dispersion in the lateral anteroposterior, medial anteroposterior, and mediolateral dimensions depending on the patient. Our data showed that the tibial components of the Genesis II and Persona rather than that of the NexGen may be preferable for Japanese people because of their asymmetric design. Copyright © 2016 The Japanese Orthopaedic Association. Published by Elsevier B.V. All rights reserved.

Lateral Variations in SKS Splitting Across the MAGIC Array, Central Appalachians

NASA Astrophysics Data System (ADS)

Aragon, John C.; Long, Maureen D.; Benoit, Margaret H.

2017-11-01

The eastern margin of North America has been shaped by several cycles of supercontinent assembly. These past episodes of orogenesis and continental rifting have likely deformed the lithosphere, but the extent, style, and geometry of this deformation remain poorly known. Measurements of seismic anisotropy in the upper mantle can shed light on past lithospheric deformation, but may also reveal contributions from present-day mantle flow in the asthenosphere. Here we examine SKS waveforms and measure splitting of SKS phases recorded by the MAGIC experiment, a dense transect of seismic stations across the central Appalachians. Our measurements constrain small-scale lateral variations in azimuthal anisotropy and reveal distinct regions of upper mantle anisotropy. Stations within the present-day Appalachian Mountains exhibit fast splitting directions roughly parallel to the strike of the mountains and delay times of about 1.0 s. To the west, transverse component waveforms for individual events reveal lateral variability in anisotropic structure. Stations immediately to the east of the mountains exhibit complicated splitting patterns, more null SKS arrivals, and a distinct clockwise rotation of fast directions. The observed variability in splitting behavior argues for contributions from both the lithosphere and the asthenospheric mantle. We infer that the sharp lateral transition in splitting behavior at the eastern edge of the Appalachians is controlled by a change in anisotropy in the lithospheric mantle. We hypothesize that beneath the Appalachians, SKS splitting reflects lithospheric deformation associated with Appalachian orogenesis, while just to the east this anisotropic signature was modified by Mesozoic rifting.

Novel approach based on one-tube nested PCR and a lateral flow strip for highly sensitive diagnosis of tuberculous meningitis.

PubMed

Sun, Yajuan; Chen, Jiajun; Li, Jia; Xu, Yawei; Jin, Hui; Xu, Na; Yin, Rui; Hu, Guohua

2017-01-01

Rapid and sensitive detection of Mycobacterium tuberculosis (M. Tb) in cerebrospinal fluid is crucial in the diagnosis of tuberculous meningitis (TBM), but conventional diagnostic technologies have limited sensitivity and specificity or are time-consuming. In this work, a novel, highly sensitive molecular diagnostic method, one-tube nested PCR-lateral flow strip test (OTNPCR-LFST), was developed for detecting M. tuberculosis. This one-tube nested PCR maintains the sensitivity of conventional two-step nested PCR and reduces both the chance of cross-contamination and the time required for analysis. The PCR product was detected by a lateral flow strip assay, which provided a basis for migration of the test to a point-of-care (POC) microfluidic format. The developed assay had an improved sensitivity compared with traditional PCR, and the limit of detection was up to 1 fg DNA isolated from M. tuberculosis. The assay was also specific for M. tuberculosis, and no cross-reactions were found in other non-target bacteria. The application of this technique to clinical samples was successfully evaluated, and OTNPCR-LFST showed 89% overall sensitivity and 100% specificity for TBM patients. This one-tube nested PCR-lateral flow strip assay is useful for detecting M. tuberculosis in TBM due to its rapidity, high sensitivity and simple manipulation.

A Novel Quantum Dots-Based Point of Care Test for Syphilis

NASA Astrophysics Data System (ADS)

Yang, Hao; Li, Ding; He, Rong; Guo, Qin; Wang, Kan; Zhang, Xueqing; Huang, Peng; Cui, Daxiang

2010-05-01

One-step lateral flow test is recommended as the first line screening of syphilis for primary healthcare settings in developing countries. However, it generally shows low sensitivity. We describe here the development of a novel fluorescent POC (Point Of Care) test method to be used for screening for syphilis. The method was designed to combine the rapidness of lateral flow test and sensitiveness of fluorescent method. 50 syphilis-positive specimens and 50 healthy specimens conformed by Treponema pallidum particle agglutination (TPPA) were tested with Quantum Dot-labeled and colloidal gold-labeled lateral flow test strips, respectively. The results showed that both sensitivity and specificity of the quantum dots-based method reached up to 100% (95% confidence interval [CI], 91-100%), while those of the colloidal gold-based method were 82% (95% CI, 68-91%) and 100% (95% CI, 91-100%), respectively. In addition, the naked-eye detection limit of quantum dot-based method could achieve 2 ng/ml of anti-TP47 polyclonal antibodies purified by affinity chromatography with TP47 antigen, which was tenfold higher than that of colloidal gold-based method. In conclusion, the quantum dots were found to be suitable for labels of lateral flow test strip. Its ease of use, sensitiveness and low cost make it well-suited for population-based on-the-site syphilis screening.

The structure of a turbulent flow in a channel of complex shape

USGS Publications Warehouse

Tracy, Hubert Jerome

1976-01-01

Measurements of the Reynolds stresses and the mean motion pattern were made in a uniform turbulent motion in a conduit consisting of a large, nearly square section joined by a smaller rectangular section. The results indicate that the boundary shearing stress is nearly constant over large segments of the boundaries. The magnitudes of the lateral and the vertical components of turbulence are not the same near a boundary and the component normal to the boundary is smaller than the component parallel to the boundary. The difference in the two components in the corner regions of the channel produces secondary mean motions in the plane of the channel section. The strength of the motion depends upon the angle subtended by the corner. A principal function of the secondary motions is to transfer momentum into the corner regions and, elsewhere, to compensate for the excess force due to the shear gradients. In the absence of the secondary motions, the fluid must stagnate and separate from the boundaries in certain regions and be greatly accelerated in others. The secondary motions are conventionally described in terms of symmetrical rotations in cells bounded by the corner bisectors. The measured motion pattern is at variance with this view, unless the symmetry is confined to a very local region. (Woodard-USGS)

Flow characteristics and spillage mechanisms of wall-mounted and jet-isolated range hoods subject to influence from cross draft.

PubMed

Chen, Jia-Kun; Huang, Rong Fung; Peng, Kuan-Lin

2012-01-01

The effects of draft on the flow and spillage characteristics of wall-mounted and jet-isolated range hoods were investigated. A specially designed draft generator that could supply low-swirl air current was used to provide "cross draft" from three directions, lateral (θ = 0(o)), oblique (θ = 45(o)), and front (θ = 90(o)), with respect to the center point of the range hoods. Flow characteristics of oil mist were inspected through visualization of smoke flows with light scattering (laser light sheet-assisted visualization of smoke flow). The leakage mechanisms, which were closely related to the flow features, were studied by examining both movies and still pictures showing smoke-flow evolution. The sulfur hexafluoride tracer gas concentration detection method was employed to measure the capture indices. The results showed that the lateral draft pushed the pollutants generated under the hood in the opposite direction and induced serious spillage. The oblique draft pushed the pollutants toward both the rear wall and opposite side and induced more serious spillage than did the lateral draft. The frontal draft forced the pollutants to bifurcate into streams moving toward the left and the right, and induced the most serious pollutant spillage among the three tested drafts. Pollutant spillage became critically significant as the cross draft velocity was increased to greater than 0.2 m/sec. Spillage of pollutants increased as the velocity of the cross draft was increased. Increasing the suction flow rate of the range hood may increase resistance to the draft, but the benefits were limited at draft velocities greater than 0.2 m/sec. Both range hoods had a similarly low capture index under the influence of the lateral draft. For the oblique and frontal drafts, the jet-isolated range hood demonstrated a higher capture index than did the wall-mounted range hood.

The effects of different lying positions on interface pressure, skin temperature, and tissue blood flow in nursing home residents.

PubMed

Källman, Ulrika; Engström, Maria; Bergstrand, Sara; Ek, Anna-Christina; Fredrikson, Mats; Lindberg, Lars-Göran; Lindgren, Margareta

2015-03-01

Although repositioning is considered an important intervention to prevent pressure ulcers, tissue response during loading in different lying positions has not been adequately explored. To compare the effects of different lying positions on interface pressure, skin temperature, and tissue blood flow in nursing home residents. From May 2011 to August 2012, interface pressure, skin temperature, and blood flow at three tissue depths were measured for 1 hr over the sacrum in 30° supine tilt and 0° supine positions and over the trochanter major in 30° lateral and 90° lateral positions in 25 residents aged 65 years or older. Measurement of interface pressure was accomplished using a pneumatic pressure transmitter connected to a digital manometer, skin temperature using a temperature sensor, and blood flow using photoplethysmography and laser Doppler flowmetry. Interface pressure was significantly higher in the 0° supine and 90° lateral positions than in 30° supine tilt and 30° lateral positions. The mean skin temperature increased from baseline in all positions. Blood flow was significantly higher in the 30° supine tilt position compared to the other positions. A hyperemic response in the post pressure period was seen at almost all tissue depths and positions. The 30° supine tilt position generated less interface pressure and allowed greater tissue perfusion, suggesting that this position is the most beneficial. © The Author(s) 2014.

A sprinkling experiment to quantify celerity-velocity differences at the hillslope scale.

PubMed

van Verseveld, Willem J; Barnard, Holly R; Graham, Chris B; McDonnell, Jeffrey J; Brooks, J Renée; Weiler, Markus

2017-01-01

Few studies have quantified the differences between celerity and velocity of hillslope water flow and explained the processes that control these differences. Here, we asses these differences by combining a 24-day hillslope sprinkling experiment with a spatially explicit hydrologic model analysis. We focused our work on Watershed 10 at the H. J. Andrews Experimental Forest in western Oregon. Celerities estimated from wetting front arrival times were generally much faster than average vertical velocities of δ 2 H. In the model analysis, this was consistent with an identifiable effective porosity (fraction of total porosity available for mass transfer) parameter, indicating that subsurface mixing was controlled by an immobile soil fraction, resulting in the attenuation of the δ 2 H input signal in lateral subsurface flow. In addition to the immobile soil fraction, exfiltrating deep groundwater that mixed with lateral subsurface flow captured at the experimental hillslope trench caused further reduction in the δ 2 H input signal. Finally, our results suggest that soil depth variability played a significant role in the celerity-velocity responses. Deeper upslope soils damped the δ 2 H input signal, while a shallow soil near the trench controlled the δ 2 H peak in lateral subsurface flow response. Simulated exit time and residence time distributions with our hillslope hydrologic model showed that water captured at the trench did not represent the entire modeled hillslope domain; the exit time distribution for lateral subsurface flow captured at the trench showed more early time weighting.

A sprinkling experiment to quantify celerity-velocity differences at the hillslope scale

NASA Astrophysics Data System (ADS)

van Verseveld, Willem J.; Barnard, Holly R.; Graham, Chris B.; McDonnell, Jeffrey J.; Renée Brooks, J.; Weiler, Markus

2017-11-01

Few studies have quantified the differences between celerity and velocity of hillslope water flow and explained the processes that control these differences. Here, we asses these differences by combining a 24-day hillslope sprinkling experiment with a spatially explicit hydrologic model analysis. We focused our work on Watershed 10 at the H. J. Andrews Experimental Forest in western Oregon. Celerities estimated from wetting front arrival times were generally much faster than average vertical velocities of δ2H. In the model analysis, this was consistent with an identifiable effective porosity (fraction of total porosity available for mass transfer) parameter, indicating that subsurface mixing was controlled by an immobile soil fraction, resulting in the attenuation of the δ2H input signal in lateral subsurface flow. In addition to the immobile soil fraction, exfiltrating deep groundwater that mixed with lateral subsurface flow captured at the experimental hillslope trench caused further reduction in the δ2H input signal. Finally, our results suggest that soil depth variability played a significant role in the celerity-velocity responses. Deeper upslope soils damped the δ2H input signal, while a shallow soil near the trench controlled the δ2H peak in lateral subsurface flow response. Simulated exit time and residence time distributions with our hillslope hydrologic model showed that water captured at the trench did not represent the entire modeled hillslope domain; the exit time distribution for lateral subsurface flow captured at the trench showed more early time weighting.

Natural and anthropogenic land cover change and its impact on the regional climate and hydrological extremes over Sanjiangyuan region

NASA Astrophysics Data System (ADS)

Ji, P.; Yuan, X.

2017-12-01

Located in the northern Tibetan Plateau, Sanjiangyuan is the headwater region of the Yellow River, Yangtze River and Mekong River. Besides climate change, natural and human-induced land cover change (e.g., Graze for Grass Project) is also influencing the regional hydro-climate and hydrological extremes significantly. To quantify their impacts, a land surface model (LSM) with consideration of soil moisture-lateral surface flow interaction and quasi-three-dimensional subsurface flow, is used to conduct long-term high resolution simulations driven by China Meteorological Administration Land Data Assimilation System forcing data and different land cover scenarios. In particular, the role of surface and subsurface lateral flows is also analyzed by comparing with typical one-dimensional models. Lateral flows help to simulate soil moisture variability caused by topography at hyper-resolution (e.g., 100m), which is also essential for simulating hydrological extremes including soil moisture dryness/wetness and high/low flows. The LSM will also be coupled with a regional climate model to simulate the effect of natural and anthropogenic land cover change on regional climate, with particular focus on the land-atmosphere coupling at different resolutions with different configurations in modeling land surface hydrology.

Interactions between Super Typhoon Megi (2010) and the Monsoon Gyre

NASA Astrophysics Data System (ADS)

Peng, M.; Li, T.; Bi, M.; Shen, X.

2015-12-01

Accurate prediction of tropical cyclone track is critical for high-impact weather preparedness, especially as the storm is near the coastal region. The track prediction for super typhoon Megi (2010) in the western Pacific was notoriously bad as most operational models predicated a mainly westward movement while Megi actually made a northward turning after it has crossed the Philippines islands. In this study, we try to understand this rather irregular motion for Megi. Examination of NCEP reanalyzed fields indicated that during this period a low-frequency (10-60-day) monsoon gyre in the vicinity of Megi may have interactions with the latter. To understand the effect of the low-frequency mode on the movement of Megi, numerical experiments were designed and conducted. The total flow from the analyzed field is separated into 1) a slowly varying background state, 2) a 10-60-day low frequency component representing the monsoon gyre, and 3) a 10-day high-pass filtered component representing Megi. In the control experiment, the total field containing all three components is used as the initial and lateral boundary conditions, and the WRF model is able to simulate Megi's sharp northward turning successfully. In the second experiment, the 10-60-day mode is removed from the initial and lateral boundary fields. In the absence of the low-frequency mode, Megi moves westward and only slightly northwestward without turning north. When the vortex representing Megi was removed, the movement of the monsoon gyre was also affected. These experiments indicated strong interactions between Megi and the monsoon gyre. The interactions and the way the monsoon gyre actually affected the track of Megi will be discussed in the presentation.

Analytical and experimental studies of flow-induced vibration of SSME components

NASA Technical Reports Server (NTRS)

Chen, S. S.; Jendrzejczyk, J. A.; Wambsganss, M. W.

1987-01-01

Components of the Space Shuttle Main Engines (SSMEs) are subjected to a severe environment that includes high-temperature, high-velocity flows. Such flows represent a source of energy that can induce and sustain large-amplitude vibratory stresses and/or result in fluidelastic instabilities. Three components are already known to have experienced failures in evaluation tests as a result of flow-induced structural motion. These components include the liquid-oxygen (LOX) posts, the fuel turbine bellows shield, and the internal inlet tee splitter vane. Researchers considered the dynamic behavior of each of these components with varying degrees of effort: (1) a theoretical and experimental study of LOX post vibration excited by a fluid flow; (2) an assessment of the internal inlet tee splitter vane vibration (referred to as the 4000-Hz vibration problem); and (3) a preliminary consideration of the bellows shield problem. Efforts to resolve flow-induced vibration problems associated with the SSMEs are summarized.

[Rationality of commodity criteria and traditional breeding of Polygala tenuifolia based on agronomic traits and determination of chemical components].

PubMed

Wang, Dan-Dan; Bai, Lu; Xu, Xiao-Shuang; Zhang, Fu-Sheng; Xing, Jie; Jia, Jin-Ping; Tian, Hong-Ling; Qin, Xue-Mei

2016-10-01

The agronomic traits (plant height, root diameter, root length, first lateral root height, lateral root amount, root weight) of 18 Polygala tenuifolia samples with different agronomic traits were analyzed, respectively. HPLC was used to analyze three main characteristic components including tenuifolin, polygalaxanthone Ⅲ, and 3,6'-disinapoyl sucrose. At last, the correlation between six agronomic traits and three main characteristic components were analyzed by scatter plot. We found no significant correlation between root diameter and three main characteristic components. There were no obvious correlations between tenuifolin and the remaining five agronomic traits. Short root length and first lateral root height as well as high lateral root amount resulted in high levels of polygalaxanthone Ⅲ in P. tenuifolia samples. High levels of 3,6'-disinapoyl sucrose were observed in P. tenuifolia samples with longer root. So, the current commodity criteria and traditional breeding of P. tenuifolia did not conform to pharmacopoeia standards, which excellent medicinal materials should have high contents of the main characteristic components. It was urgent to revise the current commodity criteria and breeding methods. Copyright© by the Chinese Pharmaceutical Association.

Cooling system for electronic components

DOEpatents

Anderl, William James; Colgan, Evan George; Gerken, James Dorance; Marroquin, Christopher Michael; Tian, Shurong

2015-12-15

Embodiments of the present invention provide for non interruptive fluid cooling of an electronic enclosure. One or more electronic component packages may be removable from a circuit card having a fluid flow system. When installed, the electronic component packages are coincident to and in a thermal relationship with the fluid flow system. If a particular electronic component package becomes non-functional, it may be removed from the electronic enclosure without affecting either the fluid flow system or other neighboring electronic component packages.

Cooling system for electronic components

DOEpatents

Anderl, William James; Colgan, Evan George; Gerken, James Dorance; Marroquin, Christopher Michael; Tian, Shurong

2016-05-17

Embodiments of the present invention provide for non interruptive fluid cooling of an electronic enclosure. One or more electronic component packages may be removable from a circuit card having a fluid flow system. When installed, the electronic component packages are coincident to and in a thermal relationship with the fluid flow system. If a particular electronic component package becomes non-functional, it may be removed from the electronic enclosure without affecting either the fluid flow system or other neighboring electronic component packages.

Development and Validation of a Lateral Flow Immunoassay Test Kit for Dual Detection of Casein and β-Lactoglobulin Residues.

PubMed

Masiri, Jongkit; Barrios-Lopez, Brianda; Benoit, Lora; Tamayo, Joshua; Day, Jeffrey; Nadala, Cesar; Sung, Shao-Lei; Samadpour, Mansour

2016-03-01

Allergies to cow's milk are very common and can present as life-threatening anaphylaxis. Consequently, food labeling legislation mandates that foods containing milk residues, including casein and/or β-lactoglobulin, provide an indication of such on the product label. Because contamination with either component independent of the other can occur during food manufacturing, effective allergen management measures for containment of milk residues necessitates the use of dual screening methods. To assist the food industry in improving food safety practices, we have developed a rapid lateral flow immunoassay test kit that reliably reports both residues down to 0.01 μg per swab and 0.1 ppm of protein for foods. The assay utilizes both sandwich and competitive format test lines and is specific for bovine milk residues. Selectivity testing using a panel of matrices with potentially interfering substances, including commonly used sanitizing agents, indicated reduction in the limit of detection by one-to fourfold. With food, residues were easily detected in all cow's milk-based foods tested, but goat and sheep milk residues were not detected. Specificity analysis revealed no cross-reactivity with common commodities, with the exception of kidney beans when present at high concentrations (> 1%). The development of a highly sensitive and rapid test method capable of detecting trace amounts of casein and/or β-lactoglobulin should aid food manufacturers and regulatory agencies in monitoring for milk allergens in environmental and food samples.

The intracellular responses of frog eggs to novel orientations to gravity

NASA Technical Reports Server (NTRS)

Radice, G. P.; Neff, A. W.; Malacinski, G. M.

1982-01-01

It is found that multiple short doses of ultraviolet light are as effective as a single large dose in producing neural defects. In addition, 180 deg rotation (inversion) of irradiated eggs reduces the ultraviolet effect. Since yolk platelets may be the gravity sensing mechanism, their size, density, and distribution in normal and inverted eggs are investigated. Large platelets are denser and for the most part are in a distinct zone in the vegetal hemisphere, whereas small platelets are less dense and occur in the animal hemisphere. When inverted, the large platelets flow into the animal hemisphere as a coherent mass and partially displace the small platelets. Inversion is thought to rearrange cytoplasmic components necessary for later neural development into an appropriate configuration.

Vortex Flap Technology: a Stability and Control Assessment

NASA Technical Reports Server (NTRS)

Carey, K. M.; Erickson, G. E.

1984-01-01

A comprehensive low-speed wind tunnel investigation was performed of leading edge vortex flaps applied to representative aircraft configurations. A determination was made of the effects of analytically- and empirically-designed vortex flaps on the static longitudinal and lateral-directional aerodynamics, stability, and control characteristics of fighter wings having leading-edge sweep angles of 45 to 76.5 degrees. The sensitivity to several configuration modifications was assessed, which included the effects of flap planform, leading- and trailing-edge flap deflection angles, wing location on the fuselage, forebody strakes, canards, and centerline and outboard vertical tails. Six-component forces and moments, wing surface static pressure distributions, and surface flow patterns were obtained using the Northrop 21- by 30-inch low-speed wind tunnel.

Results of investigations on a 0.0405 scale model ATP version of the NR-SSV orbiter in the North American Aeronautical Laboratory low speed wind tunnel

NASA Technical Reports Server (NTRS)

Mennell, R.; Vaughn, J. E.; Singellton, R.

1973-01-01

Experimental aerodynamic investigations were conducted on a scale model space shuttle vehicle (SSV) orbiter. The purpose of the test was to investigate the longitudinal and lateral-directional aerodynamic characteristics. Emphasis was placed on model component, wing-glove, and wing-body fairing effects, as well as elevon, aileron, and rudder control effectiveness. Angles of attack from - 5 deg to + 30 deg and angles of sideslip from - 5 deg to + 10 deg were tested. Static pressures were recorded on base, fuselage, and wing surfaces. Tufts and talc-kerosene flow visualization techniques were also utilized. The aerodynamic force balance results are presented in plotted and tabular form.

Quasi 3D modeling of water flow in vadose zone and groundwater

USDA-ARS?s Scientific Manuscript database

The complexity of subsurface flow systems calls for a variety of concepts leading to the multiplicity of simplified flow models. One habitual simplification is based on the assumption that lateral flow and transport in unsaturated zone are not significant unless the capillary fringe is involved. In ...

Built-In Data-Flow Integration Testing in Large-Scale Component-Based Systems

NASA Astrophysics Data System (ADS)

Piel, Éric; Gonzalez-Sanchez, Alberto; Gross, Hans-Gerhard

Modern large-scale component-based applications and service ecosystems are built following a number of different component models and architectural styles, such as the data-flow architectural style. In this style, each building block receives data from a previous one in the flow and sends output data to other components. This organisation expresses information flows adequately, and also favours decoupling between the components, leading to easier maintenance and quicker evolution of the system. Integration testing is a major means to ensure the quality of large systems. Their size and complexity, together with the fact that they are developed and maintained by several stake holders, make Built-In Testing (BIT) an attractive approach to manage their integration testing. However, so far no technique has been proposed that combines BIT and data-flow integration testing. We have introduced the notion of a virtual component in order to realize such a combination. It permits to define the behaviour of several components assembled to process a flow of data, using BIT. Test-cases are defined in a way that they are simple to write and flexible to adapt. We present two implementations of our proposed virtual component integration testing technique, and we extend our previous proposal to detect and handle errors in the definition by the user. The evaluation of the virtual component testing approach suggests that more issues can be detected in systems with data-flows than through other integration testing approaches.

An investigation on the effect of street morphology to ambient air quality using six real-world cases

NASA Astrophysics Data System (ADS)

Shen, Jialei; Gao, Zhi; Ding, Wowo; Yu, Ying

2017-09-01

Street canyons are vulnerable to air pollution mainly caused by vehicle emissions, which are therefore closely related to pedestrians' health. Previous studies have showed that air quality in street canyons is associated with street morphology, though the majority of them have focused on idealized street models. This paper attempts to investigate the relationship of street morphology to air quality for 6 irregular real-world cases selected from America, Europe, and China, i.e. Manhattan, Paris, Barcelona, Berlin, London and Nanjing. Each street is analyzed as a set of slices to propose a couple of morphology indices for quantitatively assessing the actual street morphology. Pollutant transport rate of mean flows and turbulent diffusion, net escape velocity and age of air are obtained from computational fluid dynamics (CFD) simulations to assess the ventilations and pollutant dispersion within street canyons with a parallel approaching wind. The results show that the street morphology characteristics, including the street width, lateral openings and intersections, are closely related to the air flows in street canyons. The air quality improves with a decreasing aspect ratio of central street owing to a larger vertical exchange through the street roof, which suggests an open central street is of better air quality. The lateral openings and intersections of streets have important effects on the air flows in street canyons, and the effects are particularly pronounced when the street widths are similar. The street continuity ratio indicates street continuity. It relates to the openings and the symmetry of a street and impacts on the air flows and pollutant dispersion through the lateral openings of the central street. The street spatial closure ratio is determined by the street continuity ratio and the aspect ratio of the central street. When the aspect ratio of central street is not excessively high, higher values of street continuity ratio and spatial closure ratio can lead to a stronger channel flow in street canyons and improve the air quality. The octagon intersections are favorable for air flowing through the lateral openings and improve the channel flows. The oblique intersections can also greatly improve the street ventilations, mainly due to the enhanced air flows through the lateral openings and the increased turbulent diffusion through the street roofs.

Predicting Peak Flows following Forest Fires

NASA Astrophysics Data System (ADS)

Elliot, William J.; Miller, Mary Ellen; Dobre, Mariana

2016-04-01

Following forest fires, peak flows in perennial and ephemeral streams often increase by a factor of 10 or more. This increase in peak flow rate may overwhelm existing downstream structures, such as road culverts, causing serious damage to road fills at stream crossings. In order to predict peak flow rates following wildfires, we have applied two different tools. One is based on the U.S.D.A Natural Resource Conservation Service Curve Number Method (CN), and the other is by applying the Water Erosion Prediction Project (WEPP) to the watershed. In our presentation, we will describe the science behind the two methods, and present the main variables for each model. We will then provide an example of a comparison of the two methods to a fire-prone watershed upstream of the City of Flagstaff, Arizona, USA, where a fire spread model was applied for current fuel loads, and for likely fuel loads following a fuel reduction treatment. When applying the curve number method, determining the time to peak flow can be problematic for low severity fires because the runoff flow paths are both surface and through shallow lateral flow. The WEPP watershed version incorporates shallow lateral flow into stream channels. However, the version of the WEPP model that was used for this study did not have channel routing capabilities, but rather relied on regression relationships to estimate peak flows from individual hillslope polygon peak runoff rates. We found that the two methods gave similar results if applied correctly, with the WEPP predictions somewhat greater than the CN predictions. Later releases of the WEPP model have incorporated alternative methods for routing peak flows that need to be evaluated.

Sensitive Detection of Norovirus Using Phage Nanoparticle Reporters in Lateral-Flow Assay

PubMed Central

Hagström, Anna E. V.; Garvey, Gavin; Paterson, Andrew S.; Dhamane, Sagar; Adhikari, Meena; Estes, Mary K.; Strych, Ulrich; Kourentzi, Katerina; Atmar, Robert L.; Willson, Richard C.

2015-01-01

Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundred-fold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair. PMID:25978622

The sensory basis of rheotaxis in turbulent flow

NASA Astrophysics Data System (ADS)

Elder, John P.

Rheotaxis is a robust, multisensory behavior with many potential benefits for fish and other aquatic animals, yet the influence of different fluvial conditions on rheotactic performance and its sensory basis is still poorly understood. Here, we examine the role that vision and the lateral line play in the rheotactic behavior of a stream-dwelling species (Mexican tetra, Astyanax mexicanus) under both rectilinear and turbulent flow conditions. Turbulence enhanced overall rheotactic strength and lowered the flow speed at which rheotaxis was initiated; this effect did not depend on the availability of either visual or lateral line information. Compared to fish without access to visual information, fish with access to visual information exhibited increased levels of positional stability and as a result, increased levels of rheotactic accuracy. No disruption in rheotactic performance was found when the lateral line was disabled, suggesting that this sensory system is not necessary for either rheotaxis or turbulence detection under the conditions of this study.

Localization of source with unknown amplitude using IPMC sensor arrays

NASA Astrophysics Data System (ADS)

Abdulsadda, Ahmad T.; Zhang, Feitian; Tan, Xiaobo

2011-04-01

The lateral line system, consisting of arrays of neuromasts functioning as flow sensors, is an important sensory organ for fish that enables them to detect predators, locate preys, perform rheotaxis, and coordinate schooling. Creating artificial lateral line systems is of significant interest since it will provide a new sensing mechanism for control and coordination of underwater robots and vehicles. In this paper we propose recursive algorithms for localizing a vibrating sphere, also known as a dipole source, based on measurements from an array of flow sensors. A dipole source is frequently used in the study of biological lateral lines, as a surrogate for underwater motion sources such as a flapping fish fin. We first formulate a nonlinear estimation problem based on an analytical model for the dipole-generated flow field. Two algorithms are presented to estimate both the source location and the vibration amplitude, one based on the least squares method and the other based on the Newton-Raphson method. Simulation results show that both methods deliver comparable performance in source localization. A prototype of artificial lateral line system comprising four ionic polymer-metal composite (IPMC) sensors is built, and experimental results are further presented to demonstrate the effectiveness of IPMC lateral line systems and the proposed estimation algorithms.

Comparison of isometric exercises for activating latissimus dorsi against the upper body weight.

PubMed

Park, Se-yeon; Yoo, Won-gyu; An, Duk-hyun; Oh, Jae-seop; Lee, Jung-hoon; Choi, Bo-ram

2015-02-01

Because there is little agreement as to which exercise is the most effective for activating the latissimus dorsi, and its intramuscular components are rarely compared, we investigated the intramuscular components of the latissimus dorsi during both trunk and shoulder exercises. Sixteen male subjects performed four isometric exercises: inverted row, body lifting, trunk extension, and trunk lateral bending. Surface electromyography (sEMG) was used to collect data from the medial and lateral components of the latissimus dorsi, lower trapezius, and the erector spinae at the 12th thoracic level during the isometric exercises. Two-way repeated analysis of variance with two within-subject factors (muscles and exercise conditions) was used to determine the significance of differences between the muscles and differences between exercise variations. The inverted row showed the highest values for the medial latissimus dorsi, which were significantly higher than those of the body lifting or trunk extension exercises. For the lateral latissimus dorsi, lateral bending showed significantly higher muscle activity than the inverted row or trunk extension. During body lifting, the % maximum voluntary isometric contraction (MVIC) of the erector spinae showed the lowest value, significantly lower than those of the other isometric exercises. The inverted row exercise was effective for activating the medial latissimus dorsi versus the shoulder depression and trunk exertion exercises. The lateral bending and body lifting exercises were favorable for activating the lateral component of the latissimus dorsi. Evaluating trunk lateral bending is essential for examining the function of the latissimus dorsi. Copyright © 2014 Elsevier Ltd. All rights reserved.

Hydro-dynamic damping theory in flowing water

NASA Astrophysics Data System (ADS)

Monette, C.; Nennemann, B.; Seeley, C.; Coutu, A.; Marmont, H.

2014-03-01

Fluid-structure interaction (FSI) has a major impact on the dynamic response of the structural components of hydroelectric turbines. On mid-head to high-head Francis runners, the rotor-stator interaction (RSI) phenomenon always has to be considered carefully during the design phase to avoid operational issues later on. The RSI dynamic response amplitudes are driven by three main factors: (1) pressure forcing amplitudes, (2) excitation frequencies in relation to natural frequencies and (3) damping. The prediction of the two first factors has been largely documented in the literature. However, the prediction of fluid damping has received less attention in spite of being critical when the runner is close to resonance. Experimental damping measurements in flowing water on hydrofoils were presented previously. Those results showed that the hydro-dynamic damping increased linearly with the flow. This paper presents development and validation of a mathematical model, based on momentum exchange, to predict damping due to fluid structure interaction in flowing water. The model is implemented as an analytical procedure for simple structures, such as cantilever beams, but is also implemented in more general ways using three different approaches for more complex structures such as runner blades: a finite element procedure, a CFD modal work based approach and a CFD 1DOF approach. The mathematical model and all three implementation approaches are shown to agree well with experimental results.

ULTRASTRUCTURAL STUDIES OF VASOPRESSIN EFFECT ON ISOLATED PERFUSED RENAL COLLECTING TUBULES OF THE RABBIT

PubMed Central

Ganote, Charles E.; Grantham, Jared J.; Moses, Harold L.; Burg, Maurice B.; Orloff, Jack

1968-01-01

Isolated cortical collecting tubules from rabbit kidney were studied during perfusion with solutions made either isotonic or hypotonic to the external bathing medium. Examination of living tubules revealed a reversible increase in thickness of the cellular layer, prominence of lateral cell membranes, and formation of intracellular vacuoles during periods of vasopressin-induced osmotic water transport. Examination in the electron microscope revealed that vasopressin induced no changes in cell structure in collecting tubules in the absence of an osmotic difference and significant bulk water flow across the tubule wall. In contrast, tubules fixed during vasopressin-induced periods of high osmotic water transport showed prominent dilatation of lateral intercellular spaces, bulging of apical cell membranes into the tubular lumen, and formation of intracellular vacuoles. It is concluded that the ultrastructural changes are secondary to transepithelial bulk water flow and not to a direct effect of vasopressin on the cells, and that vasopressin induces osmotic flow by increasing water permeability of the luminal cell membrane. The lateral intercellular spaces may be part of the pathway for osmotically induced transepithelial bulk water flow. PMID:4867134

Numerical simulation of the pairwise interaction of deformable cells during migration in a microchannel

NASA Astrophysics Data System (ADS)

Lan, Hongzhi; Khismatullin, Damir B.

2014-07-01

Leukocytes and other circulating cells deform and move relatively to the channel flow in the lateral and translational directions. Their migratory property is important in immune response, hemostasis, cancer progression, delivery of nutrients, and microfluidic technologies such as cell separation and enrichment, and flow cytometry. Using our three-dimensional computational algorithm for multiphase viscoelastic flow, we have investigated the effect of pairwise interaction on the lateral and translational migration of circulating cells in a microchannel. The numerical simulation data show that when two cells with the same size and small separation distance interact, repulsive interaction take place until they reach the same lateral equilibrium position. During this process, they undergo swapping or passing, depending on the initial separation distance between each other. The threshold value of this distance increases with cell deformation, indicating that the cells experiencing larger deformation are more likely to swap. When a series of closely spaced cells with the same size are considered, they generally undergo damped oscillation in both lateral and translational directions until they reach equilibrium positions where they become evenly distributed in the flow direction (self-assembly phenomenon). A series of cells with a large lateral separation distance could collide repeatedly with each other, eventually crossing the centerline and entering the other side of the channel. For a series of cells with different deformability, more deformable cells, upon impact with less deformable cells, move to an equilibrium position closer to the centerline. The results of our study show that the bulk deformation of circulating cells plays a key role in their migration in a microchannel.

Compare Vehicle Technologies | Transportation Research | NREL

Science.gov Websites

electric car diagramming energy storage, power electronics, and climate control components, as well as storage, power electronics, and climate control components, as well as energy flow among components. 3-D control components, as well as energy flow among components. 3-D illustration of electric car diagramming

Model space exploration for determining landslide source history from long period seismic data

NASA Astrophysics Data System (ADS)

Zhao, J.; Mangeney, A.; Stutzmann, E.; Capdeville, Y.; Moretti, L.; Calder, E. S.; Smith, P. J.; Cole, P.; Le Friant, A.

2012-12-01

The seismic signals generated by high magnitude landslide events can be recorded at remote stations, which provides access to the landslide process. During the "Boxing Day" eruption at Montserrat in 1997, the long-period seismic signals generated by the debris avalanche are recorded by two stations at distances of 450km and 1261km. We investigate the landslide process considering that the landslide source can be described by single forces. The period band 25-50 sec is selected for which the landslide signal is clearly visible at the two stations. We first use the transverse component of the closest station to determine the horizontal forces. We model the seismogram by normal mode summation and investigate the model space. Two horizontal forces are found that best fit the data. These two horizontal forces have similar amplitude, but opposite direction and they are separated in time by 70 sec. The radiation pattern of the transverse component does not enable to determine the exact azimuth of these forces. We then model the vertical component of the seismograms which enable to retrieve both the vertical and horizontal forces. Using the parameter previously determined (amplitude ratio and time shift of the 2 horizontal forces), we further investigate the model space and show that a single vertical force together with the 2 horizontal forces enable to fit the data. The complete source time function can be described as follows: a horizontal force toward the opposite direction of the landslide flow is followed 40 sec later by a vertical downward force and 30 more seconds later by a horizontal force toward the direction of the flow. The volume of the landslide estimated from the force magnitude is compatible with the volume determined by field survey. Inverting directly the seismograms in the period band 25-50sec enable to retrieve a source time function that is consistent with the 3 forces determined previously. The source time function in this narrow period band alone does not enable easily to recover the corresponding single forces. This method can be used to determine the source parameters using only 2 distant stations. It is successfully tested also on other landslides such as Mount St. Helens (1980) event and Mount Steller event (2005) which are recorded by more broadband stations.

Hydrodynamic interaction of two deformable drops in confined shear flow.

PubMed

Chen, Yongping; Wang, Chengyao

2014-09-01

We investigate hydrodynamic interaction between two neutrally buoyant circular drops in a confined shear flow based on a computational fluid dynamics simulation using the volume-of-fluid method. The rheological behaviors of interactive drops and the flow regimes are explored with a focus on elucidation of underlying physical mechanisms. We find that two types of drop behaviors during interaction occur, including passing-over motion and reversing motion, which are governed by the competition between the drag of passing flow and the entrainment of reversing flow in matrix fluid. With the increasing confinement, the drop behavior transits from the passing-over motion to reversing motion, because the entrainment of the reversing-flow matrix fluid turns to play the dominant role. The drag of the ambient passing flow is increased by enlarging the initial lateral separation due to the departure of the drop from the reversing flow in matrix fluid, resulting in the emergence of passing-over motion. In particular, a corresponding phase diagram is plotted to quantitatively illustrate the dependence of drop morphologies during interaction on confinement and initial lateral separation.

[The effects of carbogen inhalation on microvascular within lateral wall of cochlear following acute acoustic trauma].

PubMed

Zhao, Jing; Sun, Jianjun; Kong, Weijia

2008-11-01

To explore the influence of carbogen on lateral wall microvascular of cochlear after acute acoustic trauma. Forty guinea pigs were divided into 4 groups: group A (noise damage), group B (carbogen inhalation), group C (noise damage + carbogen inhalation), and the control group without any treatment. The diameter of the column of RBCs (RBC column diameter, RBCCD), blood flow velocity (BFV) and blood flow states(BFS) in microvasculature were measured and described under microscope. The microvascular in group A demonstrated a blood flow in contrary direction, granuliform flow, and granular slow flow. The erythrocytes aggregated in the microvascular of the cochlea. The RBCCD decreased 12.1% compared with the control group (P < 0.05). The blood flow in group B showed a laminar flow or laminar granular flow, and the RBCCD increased 20.7% compared with the control group. The blood condition in group C was the same as the control group-laminar granular blood flow; the blood flow with contrary direction was less than group A, and the RBCCD was 17.4% lager than that of group A. Carbogen can dilate the RBCCD and increase the BFV in stria vascular. So carbogen can alleviate the harm from noise.

Excess flow shutoff valve

DOEpatents

Kiffer, Micah S.; Tentarelli, Stephen Clyde

2016-02-09

Excess flow shutoff valve comprising a valve body, a valve plug, a partition, and an activation component where the valve plug, the partition, and activation component are disposed within the valve body. A suitable flow restriction is provided to create a pressure difference between the upstream end of the valve plug and the downstream end of the valve plug when fluid flows through the valve body. The pressure difference exceeds a target pressure difference needed to activate the activation component when fluid flow through the valve body is higher than a desired rate, and thereby closes the valve.

Development of Doppler Global Velocimetry as a Flow Diagnostics Tool

NASA Technical Reports Server (NTRS)

Meyers, James F.

1995-01-01

The development of Doppler global velocimetry is described from its inception to its use as a flow diagnostics tool. Its evolution is traced from an elementary one-component laboratory prototype, to a full three-component configuration operating in a wind tunnel at focal distances exceeding 15 m. As part of the developmental process, several wind tunnel flow field investigations were conducted. These included supersonic flow measurements about an oblique shock, subsonic and supersonic measurements of the vortex flow above a delta wing, and three-component measurements of a high-speed jet.

Numerical investigations on the lateral angular co-extrusion of aluminium and steel

NASA Astrophysics Data System (ADS)

Behrens, B.-A.; Klose, C.; Chugreev, A.; Thürer, S. E.; Uhe, J.

2018-05-01

In order to save weight and costs, different materials can be combined within one component. In the novel process chain being developed within the Collaborative Research Centre (CRC) 1153, joined semi-finished workpieces are used to produce hybrid solid components with locally adapted properties. Different materials are joined in an initial step before the forming process takes place. Hereby, the quality of the joining zone is improved by means of the thermo-mechanical treatment during the forming and machining processes. The lateral angular co-extrusion (LACE) approach is used to produce semi-finished workpieces because it allows for the production of coaxial semi-finished products consisting of aluminium and steel. In the further process chain, these semi-finished products are processed into hybrid bearing bushings with locally adapted properties by die forging. In the scope of this work, numerical investigations of the co-extrusion of aluminium-steel compounds were carried out using finite element (FE) simulation in order to examine the influence of the process parameters on the co-extrusion process. For this purpose, the relevant material properties of the aluminium alloy EN AW-6082 were determined experimentally and subsequently implemented in the numerical model. The obtained numerical model was used to study the impact of different ram speeds, press ratios and billet temperatures on the resulting extrusion forces and the material flow. The numerical results have been validated using force-time curves obtained from experimental extrusion tests carried out on a 2.5 MN laboratory extrusion press.

Corium shield

DOEpatents

McDonald, Douglas B.; Buchholz, Carol E.

1994-01-01

A shield for restricting molten corium from flowing into a water sump disposed in a floor of a containment vessel includes upper and lower walls which extend vertically upwardly and downwardly from the floor for laterally bounding the sump. The upper wall includes a plurality of laterally spaced apart flow channels extending horizontally therethrough, with each channel having a bottom disposed coextensively with the floor for channeling water therefrom into the sump. Each channel has a height and a length predeterminedly selected for allowing heat from the molten corium to dissipate through the upper and lower walls as it flows therethrough for solidifying the molten corium therein to prevent accumulation thereof in the sump.

Lateral Downflows in Sunspot Penumbral Filaments and their Temporal Evolution

NASA Astrophysics Data System (ADS)

Esteban Pozuelo, S.; Bellot Rubio, L. R.; de la Cruz Rodríguez, J.

2015-04-01

We study the temporal evolution of downflows observed at the lateral edges of penumbral filaments in a sunspot located very close to the disk center. Our analysis is based on a sequence of nearly diffraction-limited scans of the Fe i 617.3 nm line taken with the CRisp Imaging Spectro-Polarimeter instrument at the Swedish 1 m Solar Telescope. We compute Dopplergrams from the observed intensity profiles using line bisectors and filter the resulting velocity maps for subsonic oscillations. Lateral downflows appear everywhere in the center-side penumbra as small, weak patches of redshifts next to or along the edges of blueshifted flow channels. These patches have an intermittent life and undergo mergings and fragmentations quite frequently. The lateral downflows move together with the hosting filaments and react to their shape variations, very much resembling the evolution of granular convection in the quiet Sun. There is a good relation between brightness and velocity in the center-side penumbra, with downflows being darker than upflows on average, which is again reminiscent of quiet Sun convection. These results point to the existence of overturning convection in sunspot penumbrae, with elongated cells forming filaments where the flow is upward but very inclined, and weak lateral downward flows. In general, the circular polarization profiles emerging from the lateral downflows do not show sign reversals, although sometimes we detect three-lobed profiles that are suggestive of opposite magnetic polarities in the pixel.

Venous flow during manual lymphatic drainage applied to different regions of the lower extremity in people with and without chronic venous insufficiency: a cross-sectional study.

PubMed

Crisóstomo, R S S; Candeias, M S; Armada-da-Silva, P A S

2017-03-01

To evaluate the effect of manual lymphatic drainage (MLD) on venous flow when applied to the medial and lateral aspects of the thigh and leg in patients with chronic venous insufficiency (CVI) and healthy subjects. Cross-sectional study. Participants were assessed in a school-based health community attendant service. Fifty-seven subjects participated in this study {mean age: 43 [standard deviation (SD) 14] years, 38 women and 19 men}. Of these, 28 subjects had CVI [mean age 47 (SD 12) years] and 29 subjects did not have CVI [mean age 39 (14) years]. MLD was applied by a certificated physical therapist to the medial and lateral aspects of the thigh and leg. Cross-sectional area; blood flow velocities in the femoral vein, great saphenous vein, popliteal vein and small saphenous vein at baseline and during MLD, measured by duplex ultrasound. Flow volume in the femoral vein increased from baseline [5.19 (SD 3.25)cm 3 /second] when MLD was applied to the medial [7.03 (SD 3.65)cm 3 /second; P≤0.001; mean difference -1.69; 95% confidence interval (CI) -2.42 to -0.97] and lateral [6.16 (SD 3.35)cm 3 /second; P≤0.001; mean difference -1.04; 95% CI -1.70 to -0.39] aspects of the thigh. Venous flow augmentation in the femoral vein and great saphenous vein was higher when MLD was applied to the medial aspect of the thigh (P<0.001), while MLD had a similar effect on venous blood flow regardless of whether it was applied to the medial or the lateral aspect of the leg (P=0.731). MLD increases blood flow in deep and superficial veins. MLD should be applied along the route of the venous vessels for improved venous return. Copyright © 2016 Chartered Society of Physiotherapy. Published by Elsevier Ltd. All rights reserved.

Quantifiable Lateral Flow Assay Test Strips

NASA Technical Reports Server (NTRS)

2003-01-01

As easy to read as a home pregnancy test, three Quantifiable Lateral Flow Assay (QLFA) strips used to test water for E. coli show different results. The brightly glowing control line on the far right of each strip indicates that all three tests ran successfully. But the glowing test line on the middle left and bottom strips reveal their samples were contaminated with E. coli bacteria at two different concentrations. The color intensity correlates with concentration of contamination.

Influence of lateral discomfort on the stability of traffic flow based on visual angle car-following model

NASA Astrophysics Data System (ADS)

Zheng, Liang; Zhong, Shiquan; Jin, Peter J.; Ma, Shoufeng

2012-12-01

Due to the poor road markings and irregular driving behaviors, not every vehicle is positioned in the center of the lane. The deviation from the center can cause discomfort to drivers in the neighboring lane, which is referred to as lateral discomfort (or lateral friction). Such lateral discomfort can be incorporated into the driver stimulus-response framework by considering the visual angle and its changing rate from the psychological viewpoint. In this study, a two-lane visual angle based car-following model is proposed and its stability condition is obtained through linear stability theory. Further derivations indicate that the neutral stability line of the model is asymmetry and four factors including the vehicle width and length, the lateral separation and the sensitivity regarding the changing rate of visual angle have large impacts on the stability of traffic flow. Numerical simulations further verify these theoretical results, and demonstrate that the behaviors of diverging, merging and lane changing can break the original steady state and cause traffic fluctuations. However, these fluctuations may be alleviated to some extent by reducing the lateral discomfort.

Novel approach based on one-tube nested PCR and a lateral flow strip for highly sensitive diagnosis of tuberculous meningitis

PubMed Central

Sun, Yajuan; Chen, Jiajun; Li, Jia; Xu, Yawei; Jin, Hui; Xu, Na; Yin, Rui

2017-01-01

Rapid and sensitive detection of Mycobacterium tuberculosis (M. Tb) in cerebrospinal fluid is crucial in the diagnosis of tuberculous meningitis (TBM), but conventional diagnostic technologies have limited sensitivity and specificity or are time-consuming. In this work, a novel, highly sensitive molecular diagnostic method, one-tube nested PCR-lateral flow strip test (OTNPCR-LFST), was developed for detecting M. tuberculosis. This one-tube nested PCR maintains the sensitivity of conventional two-step nested PCR and reduces both the chance of cross-contamination and the time required for analysis. The PCR product was detected by a lateral flow strip assay, which provided a basis for migration of the test to a point-of-care (POC) microfluidic format. The developed assay had an improved sensitivity compared with traditional PCR, and the limit of detection was up to 1 fg DNA isolated from M. tuberculosis. The assay was also specific for M. tuberculosis, and no cross-reactions were found in other non-target bacteria. The application of this technique to clinical samples was successfully evaluated, and OTNPCR-LFST showed 89% overall sensitivity and 100% specificity for TBM patients. This one-tube nested PCR-lateral flow strip assay is useful for detecting M. tuberculosis in TBM due to its rapidity, high sensitivity and simple manipulation. PMID:29084241

A fluorometric lateral flow assay for visual detection of nucleic acids using a digital camera readout.

PubMed

Magiati, Maria; Sevastou, Areti; Kalogianni, Despina P

2018-06-04

A fluorometric lateral flow assay has been developed for the detection of nucleic acids. The fluorophores phycoerythrin (PE) and fluorescein isothiocyanate (FITC) were used as labels, while a common digital camera and a colored vinyl-sheet, acting as a cut-off optical filter, are used for fluorescence imaging. After DNA amplification by polymerase chain reaction (PCR), the biotinylated PCR product is hybridized to its complementary probe that carries a poly(dA) tail at 3΄ edge and then applied to the lateral flow strip. The hybrids are captured to the test zone of the strip by immobilized poly(dT) sequences and detected by streptavidin-fluorescein and streptavidin-phycoerythrin conjugates, through streptavidin-biotin interaction. The assay is widely applicable, simple, cost-effective, and offers a large multiplexing potential. Its performance is comparable to assays based on the use of streptavidin-gold nanoparticles conjugates. As low as 7.8 fmol of a ssDNA and 12.5 fmol of an amplified dsDNA target were detectable. Graphical abstract Schematic presentation of a fluorometric lateral flow assay based on fluorescein and phycoerythrin fluorescent labels for the detection of single-stranded (ssDNA) and double-stranded DNA (dsDNA) sequences and using a digital camera readout. SA: streptavidin, BSA: Bovine Serum Albumin, B: biotin, FITC: fluorescein isothiocyanate, PE: phycoerythrin, TZ: test zone, CZ: control zone.

Rapid visual detection of cyprinid herpesvirus 2 by recombinase polymerase amplification combined with a lateral flow dipstick.

PubMed

Wang, H; Sun, M; Xu, D; Podok, P; Xie, J; Jiang, Ys; Lu, Lq

2018-05-28

Herpesviral haematopoietic necrosis (HVHN), caused by cyprinid herpesvirus 2 (CyHV-2), causes significant losses in crucian carp (Carassius carassius) aquaculture. Rapid and convenient DNA assay detection of CyHV-2 is useful for field diagnosis. Recombinase polymerase amplification (RPA) is a novel isothermal DNA amplification and detection technology that can amplify DNA within 30 min at ~37°C by simulating in vivo DNA recombination. Herein, a rapid and convenient detection assay based on RPA with a lateral flow dipstick (LFD) was developed for detecting CyHV-2. The highly conserved ORF72 of CyHV-2 was targeted by specific and sensitive primers and probes. The optimized assay takes only 15 min at 38°C using a water bath, with analysis of products by 2% agarose gel electrophoresis within 30 min. A simple lateral flow strip based on the unique probe in reaction buffer was developed for visualization. The entire RPA-LFD assay takes 50 min less than the routine PCR method, is 100 times more sensitive and displays no cross-reaction with other aquatic viruses. The combined isothermal RPA and lateral flow assay (RPA-LFD) provides a simple, rapid, reliable method that could improve field diagnosis of CyHV-2 when resources are limited. © 2018 John Wiley & Sons Ltd.

Development of the GPM Observatory Thermal Vacuum Test Model

NASA Technical Reports Server (NTRS)

Yang, Kan; Peabody, Hume

2012-01-01

A software-based thermal modeling process was documented for generating the thermal panel settings necessary to simulate worst-case on-orbit flight environments in an observatory-level thermal vacuum test setup. The method for creating such a thermal model involved four major steps: (1) determining the major thermal zones for test as indicated by the major dissipating components on the spacecraft, then mapping the major heat flows between these components; (2) finding the flight equivalent sink temperatures for these test thermal zones; (3) determining the thermal test ground support equipment (GSE) design and initial thermal panel settings based on the equivalent sink temperatures; and (4) adjusting the panel settings in the test model to match heat flows and temperatures with the flight model. The observatory test thermal model developed from this process allows quick predictions of the performance of the thermal vacuum test design. In this work, the method described above was applied to the Global Precipitation Measurement (GPM) core observatory spacecraft, a joint project between NASA and the Japanese Aerospace Exploration Agency (JAXA) which is currently being integrated at NASA Goddard Space Flight Center for launch in Early 2014. From preliminary results, the thermal test model generated from this process shows that the heat flows and temperatures match fairly well with the flight thermal model, indicating that the test model can simulate fairly accurately the conditions on-orbit. However, further analysis is needed to determine the best test configuration possible to validate the GPM thermal design before the start of environmental testing later this year. Also, while this analysis method has been applied solely to GPM, it should be emphasized that the same process can be applied to any mission to develop an effective test setup and panel settings which accurately simulate on-orbit thermal environments.

Manipulation of near-wall turbulence by surface slip and permeability

NASA Astrophysics Data System (ADS)

Gómez-de-Segura, G.; Fairhall, C. T.; MacDonald, M.; Chung, D.; García-Mayoral, R.

2018-04-01

We study the effect on near-wall turbulence of tangential slip and wall-normal transpiration, typically produced by textured surfaces and other surface manipulations. For this, we conduct direct numerical simulations (DNSs) with different virtual origins for the different velocity components. The different origins result in a relative wall-normal displacement of the near-wall, quasi-streamwise vortices with respect to the mean flow, which in turn produces a change in drag. The objective of this work is to extend the existing understanding on how these virtual origins affect the flow. In the literature, the virtual origins for the tangential velocities are typically characterised by slip boundary conditions, while the wall-normal velocity is assumed to be zero at the boundary plane. Here we explore different techniques to define and implement the three virtual origins, with special emphasis on the wall-normal one. We investigate impedance conditions relating the wall-normal velocity to the pressure, and linear relations between the velocity components and their wall-normal gradients, as is typically done to impose slip conditions. These models are first tested to represent a smooth wall below the boundary plane, with all virtual origins equal, and later for different tangential and wall-normal origins. Our results confirm that the change in drag is determined by the offset between the origins perceived by mean flow and the quasi-streamwise vortices or, more generally, the near-wall turbulent cycle. The origin for the latter, however, is not set by the spanwise virtual origin alone, as previously proposed, but by a combination of the spanwise and wall-normal origins, and mainly determined by the shallowest of the two. These observations allow us to extend the existing expression to predict the change in drag, accounting for the wall-normal effect when the transpiration is not negligible.

Interactions between solidification and compositional convection in mushy layers

NASA Technical Reports Server (NTRS)

Worster, M. Grae

1994-01-01

Mushy layers are ubiquitous during the solidification of alloys. They are regions of mixed phase wherein solid crystals are bathed in the melt from which they grew. The matrix of crystals forms a porous medium through which the melt can flow, driven either by external forces or by its own buoyancy in a gravitational field. Buoyancy-driven convection of the melt depends both on temperature gradients, which are necessary for solidification, and on compositional gradients, which are generated as certain components of the alloy are preferentially incorporated in the solid phase and the remaining components are expelled into the melt. In fully liquid regions, the combined action of temperature and concentration on the density of the liquid can cause various forms of double-diffusive convection. However, in the interior of mushy regions the temperature and concentration are thermodynamically coupled so only single-diffusive convection can occur. Typically, the effect of composition on the buoyancy of the melt is much greater than the effect of temperature, and thus convection in mushy layers in driven primarily by the computational gradients within them. The rising interstitial liquid is relatively dilute, having come from colder regions of the mushy layer, where the liquidus concentration is lower, and can dissolve the crystal matrix through which it flows. This is the fundamental process by which chimneys are formed. It is a nonlinear process that requires the convective velocities to be sufficiently large, so fully fledged chimneys (narrow channels) might be avoided by means that weaken the flow. Better still would be to prevent convection altogether, since even weak convection will cause lateral, compositional inhomogeneities in castings. This report outlines three studies that examine the onset of convection within mushy layers.

Identification of runoff formation with two dyes in a mid-latitude mountain headwater

NASA Astrophysics Data System (ADS)

Vlček, Lukáš; Falátková, Kristýna; Schneider, Philipp

2017-06-01

Subsurface flow in peat bog areas and its role in the hydrologic cycle has garnered increased attention as water scarcity and floods have increased due to a changing climate. In order to further probe the mechanisms in peat bog areas and contextualize them at the catchment scale, this experimental study identifies runoff formation at two opposite hillslopes in a peaty mountain headwater; a slope with organic peat soils and a shallow phreatic zone (0.5 m below surface), and a slope with mineral Podzol soils and no detectable groundwater (> 2 m below surface). Similarities and differences in infiltration, percolation and preferential flow paths between both hillslopes could be identified by sprinkling experiments with Brilliant Blue and Fluorescein sodium. To our knowledge, this is the first time these two dyes have been compared in their ability to stain preferential flow paths in soils. Dye-stained soil profiles within and downstream of the sprinkling areas were excavated parallel (lateral profiles) and perpendicular (frontal profiles) to the slopes' gradients. That way preferential flow patterns in the soil could be clearly identified. The results show that biomat flow, shallow subsurface flow in the organic topsoil layer, occurred at both hillslopes; however, at the peat bog hillslope it was significantly more prominent. The dye solutions infiltrated into the soil and continued either as lateral subsurface pipe flow in the case of the peat bog, or percolated vertically towards the bedrock in the case of the Podzol. This study provides evidence that subsurface pipe flow, lateral preferential flow along decomposed tree roots or logs in the unsaturated zone, is a major runoff formation process at the peat bog hillslope and in the adjacent riparian zone.

Three dimensional steady subsonic Euler flows in bounded nozzles

NASA Astrophysics Data System (ADS)

Chen, Chao; Xie, Chunjing

The existence and uniqueness of three dimensional steady subsonic Euler flows in rectangular nozzles were obtained when prescribing normal component of momentum at both the entrance and exit. If, in addition, the normal component of the voriticity and the variation of Bernoulli's function at the entrance are both zero, then there exists a unique subsonic potential flow when the magnitude of the normal component of the momentum is less than a critical number. As the magnitude of the normal component of the momentum approaches the critical number, the associated flows converge to a subsonic-sonic flow. Furthermore, when the normal component of vorticity and the variation of Bernoulli function are both small, the existence and uniqueness of subsonic Euler flows with non-zero vorticity are established. The proof of these results is based on a new formulation for the Euler system, a priori estimate for nonlinear elliptic equations with nonlinear boundary conditions, detailed study for a linear div-curl system, and delicate estimate for the transport equations.

Non-hotspot volcano chains produced by migration of shear-driven upwelling toward the East Pacific Rise (Invited)

NASA Astrophysics Data System (ADS)

Ballmer, M. D.; Conrad, C. P.; Smith, E. I.; Harmon, N.

2013-12-01

While most oceanic volcanism is associated with the passive rise of hot mantle beneath the spreading axes of mid-ocean ridges (MOR), volcanism occurring off-axis reflects intraplate upper-mantle dynamics and composition, yet is poorly understood. Close to the East Pacific Rise (EPR), active magmatism propagated towards the spreading center to create a series of parallel volcanic ridges on the Pacific Plate ( ~3500 km in length for the Pukapuka, and ~500 km for the Sojourn, and Hotu-Matua ridges). Propagation of this volcanism by ~20 cm/a, as well as asymmetry in a variety of geophysical observables across the EPR, indicates strong lateral eastward pressure-driven flow in the asthenosphere; likely driven by upwelling beneath the South Pacific Superswell [1]. Although this pattern of large-scale mantle flow can account for the propagation of intraplate magmatism towards the EPR, it does not explain decompression melting itself. We hypothesize that shear-driven upwelling sustains off-axis volcanism. Unlike e.g. mantle plumes, shear-driven upwelling is a mechanism for mantle decompression that does not require lateral density heterogeneity to drive upwelling. For example, in the presence of shear across the asthenosphere, vertical flow emerges at the edges of viscosity heterogeneity [2]. These ingredients are present in the SE Pacific, where (1) shear across the asthenosphere is inferred to be greatest worldwide [2], and (2) lateral heterogeneity in mantle viscosity is indicated by geoid lineations that are associated with anomalies in seismic tomography [3]. Eastward pressure-driven flow from the South Pacific Superswell may separate into low-viscosity fingers thus providing viscosity heterogeneity [3]. Our three-dimensional numerical models [4] show that asthenospheric shear can excite upwelling and decompression melting at the tip of low-viscosity fingers that are propelled eastward by vigorous asthenospheric flow. This shear-driven upwelling is able to sustain intraplate volcanism that progresses towards the MOR, spreads laterally close to the axis, and weakly continues on the opposite plate. These predictions can explain the anomalously-fast eastward progression of volcanism, and its spatial distribution near the EPR. Moreover, for a heterogeneous mantle source involving a fertile mantle component embedded in a matrix of peridotite, the systematics of volcanism predicted by the models can account for the geochemical trend observed along the Pukapuka ridge (from C/FOZO [5] in the west toward MOR-basalt in the east), as well as the anomaly of MOR volcanism at the EPR-Pukapuka intersection (documenting C/FOZO influence). Our study highlights the role of horizontal asthenospheric flow and mantle heterogeneity in producing linear chains of intraplate volcanism independent of a (deep-rooted) buoyancy source. [1] Conder, J. A., D. W. Forsyth, E. M. Parmentier (2002): J. Geophys. Res., 107(B12), 2344. [2] Conrad, C. P., T. A. Bianco, E. I. Smith, P. Wessel (2011): Nature Geosci., 4, 317-321. [3] Harmon, N., D. W. Forsyth, D. S. Weeraratne, Y. Yang, S. C. Webb (2011): Earth Planet. Sci. Lett., 311, 306-315. [4] Ballmer, M. D., C. P. Conrad, E. I. Smith, N. Harmon (2013): Geology, 41, 479-482. [5] Zindler, A., Hart, S., 1986. Earth Planet. Sci. Lett., 14, 493-571.

Multi-Nozzle Base Flow Model in the 10- by 10-Foot Supersonic Wind Tunnel

NASA Image and Video Library

1964-02-21

Researchers check the setup of a multi-nozzle base flow model in the 10- by 10-Foot Supersonic Wind Tunnel at the National Aeronautics and Space Administration (NASA) Lewis Research Center. NASA researchers were struggling to understand the complex flow phenomena resulting from the use of multiple rocket engines. Robert Wasko and Theodore Cover of the Advanced Development and Evaluation Division’s analysis and operations sections conducted a set of tests in the 10- by 10 tunnel to further understand the flow issues. The Lewis researchers studied four and five-nozzle configurations in the 10- by 10 at simulated altitudes from 60,000 to 200,000 feet. The nozzles were gimbaled during some of the test runs to simulate steering. The flow field for the four-nozzle clusters was surveyed in the center and the lateral areas between the nozzles, whereas the five-nozzle cluster was surveyed in the lateral area only.

The effect of gas and fluid flows on nonlinear lateral vibrations of rotating drill strings

NASA Astrophysics Data System (ADS)

Khajiyeva, Lelya; Kudaibergenov, Askar; Kudaibergenov, Askat

2018-06-01

In this work we develop nonlinear mathematical models describing coupled lateral vibrations of a rotating drill string under the effect of external supersonic gas and internal fluid flows. An axial compressive load and a torque also affect the drill string. The mathematical models are derived by the use of Novozhilov's nonlinear theory of elasticity with implementation of Hamilton's variation principle. Expressions for the gas flow pressure are determined according to the piston theory. The fluid flow is considered as added mass inside the curved tube of the drill string. Using an algorithm developed in the Mathematica computation program on the basis of the Galerkin approach and the stiffness switching method the numerical solution of the obtained approximate differential equations is found. Influences of the external loads, drill string angular speed of rotation, parameters of the gas and fluid flows on the drill string vibrations are shown.

The investigation of the lateral interaction effect's on traffic flow behavior under open boundaries

NASA Astrophysics Data System (ADS)

Bouadi, M.; Jetto, K.; Benyoussef, A.; El Kenz, A.

2017-11-01

In this paper, an open boundaries traffic flow system is studied by taking into account the lateral interaction with spatial defects. For a random defects distribution, if the vehicles velocities are weakly correlated, the traffic phases can be predicted by considering the corresponding inflow and outflow functions. Conversely, if the vehicles velocities are strongly correlated, a phase segregation appears inside the system's bulk which induces the maximum current appearance. Such velocity correlation depends mainly on the defects densities and the probabilities of lateral deceleration. However, for a compact defects distribution, the traffic phases are predictable by using the inflow in the system beginning, the inflow entering the defects zone and the outflow function.

Subaqueous ice-contact fans: Depositional systems characterised by highly aggradational supercritical flow conditions

NASA Astrophysics Data System (ADS)

Lang, Joerg; Winsemann, Jutta

2015-04-01

Subaqueous ice-contact fans are deposited by high-energy plane-wall jets from subglacial conduits into standing water bodies. Highly aggradational conditions during flow expansion and deceleration allow for the preservation of bedforms related to supercritical flows, which are commonly considered rare in the depositional record. We present field examples from gravelly and sandy subaqueous ice-contact fan successions, which indicate that deposition by supercritical flows might be considered as a characteristic feature of these depositional systems. The studied successions were deposited in deep ice-dammed lakes, which formed along the margins of the Middle Pleistocene Scandinavian ice sheets across Northern Germany. The gravel-rich subaqueous fan deposits are dominated by large scour-fills (up to 25 m wide and 3 m) deep and deposits of turbulent hyperconcentrated flows, which are partly attributed to supercritical flow conditions (Winsemann et al., 2009). Scours (up to 4.5 m wide and 0.9 m deep) infilled by gravelly backsets are observed above laterally extensive erosional surfaces and are interpreted as deposits of cyclic steps. Laterally discontinuous beds of low-angle cross-stratified gravel are interpreted as antidune deposits. Downflow and up-section the gravel-rich deposits pass into sand-rich successions, which include deposits of chutes-and-pools, breaking antidunes, stationary antidunes and humpback dunes (Lang and Winsemann, 2013). Deposits of chutes-and-pools and breaking antidunes are characterised by scour-fills (up to 4 m wide and 1.2 m deep) comprising backsets or gently dipping sigmoidal foresets. Stationary antidune deposits consist of laterally extensive sinusoidal waveforms with long wavelengths (1-12 m) and low amplitudes (0.1-0.5 m), which formed under quasi-steady flows at the lower limit of the supercritical flow stage and high rates of sedimentation. Humpback dunes are characterised by divergent sigmoidal foresets and are interpreted as bedforms related to transcritical flow conditions. Deposits of aggrading stationary antidunes and humpback dunes represent a characteristic facies association of the distal zone of flow transition. Downflow the succession passes into deposits of large 3D dunes and climbing ripples. The large-scale lateral and vertical successions of bedforms are interpreted as representing the temporal and spatial evolution of the supercritical meltwater jets, which was affected by hydraulic jumps. Small-scale facies changes and the formation of individual bedforms are interpreted as controlled by fluctuating discharge, pulsating unstable flows and bed topography. References: Lang, J., Winsemann, J. (2013) Lateral and vertical facies relationships of bedforms deposited by aggrading supercritical flows: from cyclic steps to humpback dunes. Sedimentary Geology 296, 36-54. Winsemann, J., Hornung, J.J., Meinsen, J., Asprion, U., Polom, U., Brandes, C., Bussmann, M., Weber, C., (2009) Anatomy of a subaqueous ice-contact fan and delta complex, Middle Pleistocene, North-west Germany, Sedimentology 56, 1041-1076.

Development of Lateral Flow Immunochromatographic Strips for Micropollutant Screening Using Colorants of Aptamer-Functionalized Nanogold Particles, Part II: Experimental Verification with Aflatoxin B1 and Chloramphenicol.

PubMed

Zhang, Shan; Zhao, Shuai; Wang, Sai; Liu, Jiahui; Dong, Yiyang

2018-05-09

Lateral flow immunochromatographic strips based on colorants of aptamer-functionalized nanogold particles weredeveloped for the detection of micropollutants aflatoxin B1 (AFB1) and chloramphenicol (CAP). The lateral flow immunochromatographic strip was based on a competitive reaction of thiolated-aptamer between micropollutants and bio-DNA probe-streptavidin as capture material immobilized at the test line. General crucial parameters that might influence the sensitivity have been systematically investigated. To test the effectiveness and applicability of the optimized conditions, two structurally unrelated micropollutants, that is, AFB1 and CAP, were chosen for detection. In the present study, lateral flow immunochromatographic strips for AFB1 and CAP analysis by combining the high selectivity and affinity of aptamers with the unique optical properties of nanogold in municipal water samples were reported for the first time. With the optimized conditions, the immunochromatographic strip showed a visual LOD of 10 ppb and a quantitative LOD of 1.05 ppb using an immunochromatographic reader for AFB1 detection and a quantitative LOD of 63.4 ppb using an immunochromatographic reader for CAP detection. Furthermore, the sensitive strip provided a good linear detection range of approximately 0-50 ppm for AFB1 detection and a wider liner detection range of approximately 0-160 ppm for CAP detection. Moreover, the immunochromatographic strip provided recovery rates for water samples of 90-110% in the AFB1 analysis and 84-108% in the CAP analysis. The results demonstrated that the immunochromatographic strip has excellent potential for wide applicability and verified that the strip methods for the optimized conditions are applicable to a variety of micropollutants. The lateral flow immunochromatographic strip could be used as a simple, rapid, and efficient screening tool for rapid on-site detection of a variety of micropollutants.

Modeling solute transport in a heterogeneous unsaturated porous medium under dynamic boundary conditions on different spatial scales

NASA Astrophysics Data System (ADS)

Cremer, Clemens; Neuweiler, Insa; Bechtold, Michel

2013-04-01

Understanding transport of solutes/contaminants through unsaturated soil in the shallow subsurface is vital to assess groundwater quality, nutrient cycling or to plan remediation projects. Alternating precipitation and evaporation conditions causing upward and downward flux with differing flow paths, changes in saturation and related structural heterogeneity make the description of transport in the unsaturated zone near the soil-surface a complex problem. Preferential flow paths strongly depend, among other things, on the saturation of a medium. Recent studies (e.g. Bechtold et al., 2011) showed lateral flow and solute transport during evaporation conditions (upward flux) in vertically layered sand columns. Results revealed that during evaporation water and solute are redistributed laterally from coarse to fine media deeper in the soil, and towards zones of lowest hydraulic head near to the soil surface. These zones at the surface can be coarse or fine grained depending on saturation status and evaporation flux. However, if boundary conditions are reversed and precipitation is applied, the flow field is not reversed in the same manner, resulting in entirely different transport patterns for downward and upward flow. Therefore, considering net-flow rates alone is misleading when describing transport in the shallow unsaturated zone. In this contribution, we analyze transport of a solute in the shallow subsurface to assess effects resulting from the superposition of heterogeneous soil structures and dynamic flow conditions on various spatial scales. Two-dimensional numerical simulations of unsaturated flow and transport in heterogeneous porous media under changing boundary conditions are carried out using a finite-volume code coupled to a particle tracking algorithm to quantify solute transport and leaching rates. In order to validate numerical simulations, results are qualitatively compared to those of a physical experiment (Bechtold et al., 2011). Numerical simulations differ in lateral scale reaching from 0.2 m to 1.5 m, while the height of the domain is kept constant to 1.5m. Strong material heterogeneity is realized through vertical layers of coarse and fine sand. Both materials remain permanently under liquid-flow-dominated ('stage1') evaporation conditions. Spatial moments as well as the dilution index (Kitanidis, 1994) are used for quantification of transport behaviour. Results show that, while all simulations led to anomalous transport, infiltration-evaporation cycles lead to faster solute leaching rates than solely infiltration at the same net-infiltration rate in both homogeneous and heterogeneous media. Flow and transport-paths significantly differed between infiltration and evaporation, resulting in lateral water fluxes and hence lateral solute transport. Variation of the width of the model domain shows faster leaching rates for domains with small horizontal extent.

Using 81Kr and noble gases to characterize and date groundwater and brines in the Baltic Artesian Basin on the one-million-year timescale

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gerber, Christoph; Vaikmae, Rein; Aeschbach, Werner

Analyses for 81Kr and noble gases on groundwater from the deepest aquifer system of the Baltic Artesian Basin (BAB) were performed to determine groundwater ages and uncover the flow dynamics of the system on a timescale of several hundred thousand years. We find that the system is controlled by mixing of three distinct water masses: Interglacial or recent meteoric water (δ 18O ≈ –10.4‰) with a poorly evolved chemical and noble gas signature, glacial meltwater (δ 18O ≤ –18‰) with elevated noble gas concentrations, and an old, high-salinity brine component (δ 18O ≥ –4.5‰, ≥ 90 g Cl –/L) withmore » strongly depleted atmospheric noble gas concentrations. The 81Kr measurements are interpreted within this mixing framework to estimate the age of the end-members. Deconvoluted 81Kr ages range from 300 ka to 1.3 Ma for interglacial or recent meteoric water and glacial meltwater. For the brine component, ages exceed the dating range of the ATTA-3 instrument of 1.3 Ma. The radiogenic noble gas components 4He* and 40Ar* are less conclusive but also support an age of > 1 Ma for the brine. Based on the chemical and noble gas concentrations and the dating results, we conclude that the brine originates from evaporated seawater that has been modified by later water–rock interaction. Furthermore, as the obtained tracer ages cover several glacial cycles, we discuss the impact of the glacial cycles on flow patterns in the studied aquifer system.« less

Using 81Kr and noble gases to characterize and date groundwater and brines in the Baltic Artesian Basin on the one-million-year timescale

DOE PAGES

Gerber, Christoph; Vaikmae, Rein; Aeschbach, Werner; ...

2017-01-31

Analyses for 81Kr and noble gases on groundwater from the deepest aquifer system of the Baltic Artesian Basin (BAB) were performed to determine groundwater ages and uncover the flow dynamics of the system on a timescale of several hundred thousand years. We find that the system is controlled by mixing of three distinct water masses: Interglacial or recent meteoric water (δ 18O ≈ –10.4‰) with a poorly evolved chemical and noble gas signature, glacial meltwater (δ 18O ≤ –18‰) with elevated noble gas concentrations, and an old, high-salinity brine component (δ 18O ≥ –4.5‰, ≥ 90 g Cl –/L) withmore » strongly depleted atmospheric noble gas concentrations. The 81Kr measurements are interpreted within this mixing framework to estimate the age of the end-members. Deconvoluted 81Kr ages range from 300 ka to 1.3 Ma for interglacial or recent meteoric water and glacial meltwater. For the brine component, ages exceed the dating range of the ATTA-3 instrument of 1.3 Ma. The radiogenic noble gas components 4He* and 40Ar* are less conclusive but also support an age of > 1 Ma for the brine. Based on the chemical and noble gas concentrations and the dating results, we conclude that the brine originates from evaporated seawater that has been modified by later water–rock interaction. Furthermore, as the obtained tracer ages cover several glacial cycles, we discuss the impact of the glacial cycles on flow patterns in the studied aquifer system.« less

Connectivity in modelling subsurface stormflow and tracer transport in a forested hillslope

NASA Astrophysics Data System (ADS)

Laine-Kaulio, Hanne; Koivusalo, Harri

2016-04-01

Preferential flow characterizes subsurface water movement in forested hillslopes. Connectivity of preferential flowpaths in soil, as well as connections between the preferential flowpaths and the soil matrix, control the flow patterns in hillslopes. We simulated subsurface stormflow and tracer transport within a forested hillslope section in Eastern Finland with a modifiable and detailed, physically-based 3-D dual-permeability model. The objective was to investigate the role of flowpath connectivity in capturing the observed migration velocity and concentration status of a tracer plume during the different stages of an irrigation experiment. Hypotheses to explain the observed transport behaviour were formulated based on observed spatial variations in the soil properties. The hypotheses were then tested by running corresponding model versions against spatial high-frequency data from the irrigation experiment. The 3-D distribution of preferential flowpaths, and the connections and disconnections between them, had the most crucial role in capturing the observed transport event with the model. Lateral by-pass flow in the preferential flow network and the transmissivity feedback phenomenon dominated the tracer transport. However, disconnections in lateral preferential flow restrained the transport, making the transport route devious instead of being directly lateral. These disconnections were caused by spots of cemented soil material. Stones had an opposite effect on the flow route formation compared to the cemented spots because preferential flowpaths were present on stone surfaces. Preferential flowpaths related to stones were linked with other types of preferential flowpaths in soil, facilitating the connectivity of preferential flowpaths within the entire hillslope. To take into account the discontinuity in lateral preferential flow and the tortuosity of the dominant flow routes in the model, an explicit representation of the 3-D distribution of saturated hydraulic conductivity in the preferential flow network was required. In addition, with-depth changing porosity values of both pore domains of soil, i.e., the preferential flowpaths and the soil matrix, as well as explicit descriptions of local water losses into the underlying bedrock, were found important for successful simulations. Other investigated model features, including complexity in the values of the rest of the model parameters and in the calculation routines used in describing the flow connections between the preferential flowpaths and the soil matrix, were not found beneficial for the model outcome.

Bereitschaftspotential as an indicator of movement preparation in supplementary motor area and motor cortex.

PubMed

Deecke, L

1987-01-01

Topographical studies in humans of the Bereitschaftspotential (BP, or readiness potential, as averaged from the electroencephalogram) and the Bereitschaftsmagnetfeld (BM, or readiness magnetic field, as averaged from the magnetoencephalogram) revealed a widespread distribution of motor preparation over both hemispheres even before unilateral movement. This indicates the existence of several generators responsible for the BP, including generators in the ipsilateral hemisphere, which is in agreement with measurements of regional cerebral blood flow or regional cerebral energy metabolism. Nevertheless, two principal generators seem to prevail: (1) An early generator, starting its activity 1s or more before the motor act, with its maximum at the vertex. For this and other reasons, early BP generation probably stems from cortical tissue representing or including the supplementary motor area (SMA). (2) A later generator, starting its activity about 0.5s before the onset of movement and biased towards the contralateral hemisphere (contralateral preponderance of negativity, CPN). For unilateral finger movements the CPN succeeds the BP's initial bilateral symmetry in the later preparation period. Thus, this lateralized BP component probably stems from the primary motor area, MI (area 4, hand representation). While regional cerebral blood flow or regional cerebral energy metabolism show that the SMA is active in conjunction with motor acts, these data do not permit the conclusion that SMA activity precedes motor acts. This can only be shown by the Bereitschaftspotential, which proves that SMA activity occurs before the onset of movement and, what is more, before the onset of MI activity. This important order of events (first SMA, then MI activation) has been elucidated by our BP studies. It gives the SMA an important functional role: the initiation of voluntary movement. The recording of movement-related potentials associated with manual hand-tracking and motor learning points to the SMA and frontal cortex having an important role in these functions.

A microfluidic separation platform using an array of slanted ramps

NASA Astrophysics Data System (ADS)

Risbud, Sumedh; Bernate, Jorge; Drazer, German

2013-03-01

The separation of the different components of a sample is a crucial step in many micro- and nano-fluidic applications, including the detection of infections, the capture of circulating tumor cells, the isolation of proteins, RNA and DNA, to mention but a few. Vector chromatography, in which different species migrate in different directions in a planar microfluidic device thus achieving spatial as well as temporal resolution, offers the promise of high selectivity along with high throughput. In this work, we present a microfluidic vector chromatography platform consisting of slanted ramps in a microfluidic channel for the separation of suspended particles. We construct these ramps using inclined UV lithography, such that the inclined portion of the ramps is upstream. We show that particles of different size displace laterally to a different extent when driven by a flow field over a slanted ramp. The flow close to the ramp reorients along the ramp, causing the size-dependent deflection of the particles. The cumulative effect of an array of these ramps would cause particles of different size to migrate in different directions, thus allowing their passive and continuous separation.

Combined action of transverse oscillations and uniform cross-flow on vortex formation and pattern of a circular cylinder

NASA Astrophysics Data System (ADS)

Lam, K. M.; Liu, P.; Hu, J. C.

2010-07-01

This paper attempts to study the roles of lateral cylinder oscillations and a uniform cross-flow in the vortex formation and wake modes of an oscillating circular cylinder. A circular cylinder is given lateral oscillations of varying amplitudes (between 0.28 and 1.42 cylinder-diameters) in a slow uniform flow stream (Reynolds number=284) to produce the 2S, 2P and P+S wake modes. Detailed flow information is obtained with time-resolved particle-image velocimetry and the phase-locked averaging techniques. In the 2S and 2P mode, the flow speeds relative to the cylinder movement are less than the uniform flow velocity and it is found that initial formation of a vortex is caused by shear-layer separation of the uniform flow on the cylinder. Subsequent development of the shear-layer vortices is affected by the lateral cylinder movement. At small cylinder oscillation amplitudes, vortices are shed in synchronization with the cylinder movement, resulting in the 2S mode. The 2P mode occurs at larger cylinder oscillation amplitudes at which each shear-layer vortex is found to undergo intense stretching and eventual bifurcation into two separate vortices. The P+S mode occurs when the cylinder moving speeds are, for most of the time, higher than the speed of the uniform flow. These situations are found at fast and large-amplitude cylinder oscillations in which the flow relative to the cylinder movement takes over the uniform flow in governing the initial vortex formation. The formation stages of vortices from the cylinder are found to bear close resemblance to those of a vortex street pattern of a cylinder oscillating in an otherwise quiescent fluid at Keulegan-Carpenter numbers around 16. Vortices in the inclined vortex street pattern so formed are then convected downstream by the uniform flow as the vortex pairs in the 2P mode.

Effect of wing-tip dihedral on the longitudinal and lateral aerodynamic characteristics of a supersonic cruise configuration at subsonic speeds

NASA Technical Reports Server (NTRS)

Washburn, K. E.; Gloss, B. B.

1976-01-01

Force and moment data studies were conducted to determine the effect of wing-tip dihedral on the longitudinal and lateral aerodynamic characteristics of a supersonic cruise fighter configuration. Oil flow studies were also performed to investigate the model surface flow. Three models were tested: a flat (0 deg dihedral) wing tip, a dihedral, and an anhedral wing tip. The tests were conducted at the NASA Langley high-speed 7- by 10-foot wind tunnel.

Overview on Pendeo-Epitaxy of GaN-Based Heterostructures for Novel Devices Applications

DTIC Science & Technology

2006-11-01

pendeo-epitaxy uses the metal organic chemical vapor deposition (MOCVD) technique that commonly requires ammonia (NH3) and trimethyl gallium ( TMG ...lateral growth rate and the crystallography of the side walls of the pendeo-epitaxial GaN are the growth temperature, the ammonia to TMG flow rate...pressure of 100 Torr and V:III ratio of 2600. It is known that the ammonia to TMG (V:III) molar flow rate ratio plays a major role for the lateral to

On the structure of cellular solutions in Rayleigh-Benard-Marangoni flows in small-aspect-ratio containers

NASA Technical Reports Server (NTRS)

Dijkstra, Henk A.

1992-01-01

Multiple steady flow patterns occur in surface-tension/buoyancy-driven convection in a liquid layer heated from below (Rayleigh-Benard-Marangoni flows). Techniques of numerical bifurcation theory are used to study the multiplicity and stability of two-dimensional steady flow patterns (rolls) in rectangular small-aspect-ratio containers as the aspect ratio is varied. For pure Marangoni flows at moderate Biot and Prandtl number, the transitions occurring when paths of codimension 1 singularities intersect determine to a large extent the multiplicity of stable patterns. These transitions also lead, for example, to Hopf bifurcations and stable periodic flows for a small range in aspect ratio. The influence of the type of lateral walls on the multiplicity of steady states is considered. 'No-slip' lateral walls lead to hysteresis effects and typically restrict the number of stable flow patterns (with respect to 'slippery' sidewalls) through the occurrence of saddle node bifurcations. In this way 'no-slip' sidewalls induce a selection of certain patterns, which typically have the largest Nusselt number, through secondary bifurcation.

Preferential flow across scales: how important are plot scale processes for a catchment scale model?

NASA Astrophysics Data System (ADS)

Glaser, Barbara; Jackisch, Conrad; Hopp, Luisa; Klaus, Julian

2017-04-01

Numerous experimental studies showed the importance of preferential flow for solute transport and runoff generation. As a consequence, various approaches exist to incorporate preferential flow in hydrological models. However, few studies have applied models that incorporate preferential flow at hillslope scale and even fewer at catchment scale. Certainly, one main difficulty for progress is the determination of an adequate parameterization for preferential flow at these spatial scales. This study applies a 3D physically based model (HydroGeoSphere) of a headwater region (6 ha) of the Weierbach catchment (Luxembourg). The base model was implemented without preferential flow and was limited in simulating fast catchment responses. Thus we hypothesized that the discharge performance can be improved by utilizing a dual permeability approach for a representation of preferential flow. We used the information of bromide irrigation experiments performed on three 1m2 plots to parameterize preferential flow. In a first step we ran 20.000 Monte Carlo simulations of these irrigation experiments in a 1m2 column of the headwater catchment model, varying the dual permeability parameters (15 variable parameters). These simulations identified many equifinal, yet very different parameter sets that reproduced the bromide depth profiles well. Therefore, in the next step we chose 52 parameter sets (the 40 best and 12 low performing sets) for testing the effect of incorporating preferential flow in the headwater catchment scale model. The variability of the flow pattern responses at the headwater catchment scale was small between the different parameterizations and did not coincide with the variability at plot scale. The simulated discharge time series of the different parameterizations clustered in six groups of similar response, ranging from nearly unaffected to completely changed responses compared to the base case model without dual permeability. Yet, in none of the groups the simulated discharge response clearly improved compared to the base case. Same held true for some observed soil moisture time series, although at plot scale the incorporation of preferential flow was necessary to simulate the irrigation experiments correctly. These results rejected our hypothesis and open a discussion on how important plot scale processes and heterogeneities are at catchment scale. Our preliminary conclusion is that vertical preferential flow is important for the irrigation experiments at the plot scale, while discharge generation at the catchment scale is largely controlled by lateral preferential flow. The lateral component, however, was already considered in the base case model with different hydraulic conductivities in different soil layers. This can explain why the internal behavior of the model at single spots seems not to be relevant for the overall hydrometric catchment response. Nonetheless, the inclusion of vertical preferential flow improved the realism of internal processes of the model (fitting profiles at plot scale, unchanged response at catchment scale) and should be considered depending on the intended use of the model. Furthermore, we cannot exclude with certainty yet that the quantitative discharge performance at catchment scale cannot be improved by utilizing a dual permeability approach, which will be tested in parameter optimization process.

Electromagnetic deep-probing (100-1000 KMS) of the Earth's interior from artificial satellites: Constraints on the regional emplacement of crustal resources

NASA Technical Reports Server (NTRS)

Hermance, J. F. (Principal Investigator)

1981-01-01

An algorithm was developed to address the problem of electromagnetic coupling of ionospheric current systems to both a homogeneous Earth having finite conductivity, and to an Earth having gross lateral variations in its conductivity structure, e.g., the ocean-land interface. Typical results from the model simulation for ionospheric currents flowing parallel to a representative geologic discontinuity are shown. Although the total magnetic field component at the satellite altitude is an order of magnitude smaller than at the Earth's surface (because of cancellation effects from the source current), the anomalous behavior of the satellite observations as the vehicle passes over the geologic contact is relatively more important pronounced. The results discriminate among gross lithospheric structures because of difference in electrical conductivity.

Results of investigations on a 0.0405 scale model PRR version of the NR-SSV orbiter in the North American Aeronautical Laboratory low speed wind tunnel

NASA Technical Reports Server (NTRS)

Kingsland, R. B.; Vaughn, J. E.; Singellton, R.

1973-01-01

Experimental aerodynamic investigations were conducted in a low speed wind tunnel on a scale model space shuttle vehicle (SSV) orbiter. The purpose of the test was to investigate the longitudinal and lateral-directional aerodynamic characteristics of the space shuttle orbiter. Emphasis was placed on model component, wing-glove, and wing-body fairing effects, as well as elevon, aileron, and rudder control effectiveness. Angles of attack from - 5 deg to + 30 deg and angles of sideslip of - 5 deg, 0 deg, and + 5 deg were tested. Static pressures were recorded on base, fuselage, and wing surfaces. Tufts and talc-kerosene flow visualization techniques were also utilized. The aerodynamic force balance results are presented in plotted and tabular form.

Topographically Driven Lateral Water Fluxes and Their Influence on Carbon Assimilation of a Black Spruce Ecosystem.

NASA Astrophysics Data System (ADS)

Govind, A.; Chen, J. M.; Margolis, H.; Bernier, P. Y.

2006-12-01

Current estimates of ecophysiological indicators overlook the effects of topographically-driven lateral flow of soil water. We hypothesize that topographically driven lateral water flows over the landscape have significant influence on the terrestrial carbon cycle. To this end, we simulated the hydrological controls on carbon cycle processes in a black spruce forest in central Quebec, Canada, using the Boreal Ecosystem Productivity Simulator (BEPS) at a daily time step. We accounted for lateral surface and subsurface flows in BEPS by incorporating a distributed, process-oriented hydrological procedure. The results show that modeled dynamics of ecophysiological processes such as evapotranspiration (ET) and photosynthesis (GPP) are consistent with the spatial variation of land cover, topography, soil texture, and leaf area index. Simulated ET and GPP averaged within the footprint of an eddy covariance tower in the watershed agree well with flux measurements with R2=0.77 and 0.83 for ET and GPP, respectively. For ET simulation, much of the remaining discrepancies are found in the winter when the model underestimates snow sublimation. For GPP, there is an underestimation in the fall coinciding with a mid growing season drought, showing the high sensitivity of the model to the soil water status. The key processes controlling primary production were hydraulic limitations for water transfer from soil, roots, stems and leaves through stomatal conductance. Therefore, a further understanding of soil water dynamics is warranted. Comparison with the soil water content of the footprint- averaged unsaturated zone showed that the model captured the annual trend. We also simulated the variations in the water table as well as the mid growing season drought, with a reasonable accuracy(R2=0.68). The foot print average water budget reveals that the annual precipitation of 835mm is partitioned into 282mm of ET, 541 mm of subsurface runoff, and 6 mm of storage change. To test the influence of topographically driven lateral water flow on the carbon cycle, we made three hydrological modeling scenarios viz. 1) explicit hydrological simulation including lateral water routing, 2) bucket model with implicit runoff calculations and 3) a control run, where the lateral water flow was turned off in the model. Bucket model overestimated GPP as much as 25% as opposed to explicit simulations because there was no topographical constrain on runoff. Flat areas dominated with mineral soils shows the highest overestimation because of an increase in stomatal conductance. Control simulation, on the other hand, underestimated GPP as much as 15% as opposed to explicit routing because of rapid soil saturation, which decreases stomatal conductance. These results suggest that lateral water flow does play a significant role in the terrestrial carbon cycle and should be accounted for in ecological models. For details please see http://ajit.govind.googlepages.com/agu2006

Scanning Probe Microscopy for Identifying the Component Materials of a Nanostripe Structure

NASA Astrophysics Data System (ADS)

Mizuno, Akira; Ando, Yasuhisa

2010-08-01

The authors prepared a nanostripe structure in which two types of metal are arranged alternately, and successfully identified the component materials using scanning probe microscopy (SPM) to measure the lateral force distribution image. The nanostripe structure was prepared using a new method developed by the authors and joint development members. The lateral force distribution image was measured in both friction force microscopy (FFM) and lateral modulation friction force microscopy (LM-FFM) modes. In FFM mode, the effect of slope angle appeared in the lateral force distribution image; therefore, no difference in the type of material was observed. On the other hand, in LM-FFM mode, the effect of surface curvature was observed in the lateral force distribution image. A higher friction force on chromium than on gold was identified, enabling material identification.

Tire-soil interaction model for turning (steered) tires

NASA Astrophysics Data System (ADS)

Karafiath, L. L.

1985-07-01

A review of the experimental information on the development of lateral forces on tires traveling at an angle to their center plane is presented and the usefulness of the consideration of the lateral forces for the development of an analytical model is evaluated. Major components of the lateral force have been identified as the forces required to balance the tractive force and the drawbar pull vectorially. These are the shear stresses developing in the contact area and the horizontal component of the normal stresses acting on the in-ground portion or the curved side walls of the tire. The tire-soil interaction model for steady state straight travel has been expanded to include the necessary algorithms for the calculation of these lateral forces. The pattern of tractive force-slip and longitudinal-lateral force relationships is in general agreement with experiments.

Wagon instability in long trains

NASA Astrophysics Data System (ADS)

Cole, Colin; McClanachan, Mitchell; Spiryagin, Maksym; Sun, Yan Quan

2012-01-01

Lateral force components and impacts from couplers can adversely affect wagon stability. These issues are significant in longer and heavier trains increasing the risk of wagon rollover, wheel climb, wagon body pitch, bogie pitch and wagon lift-off. Modelling of coupler angles has been added to normal longitudinal train simulation to allow comprehensive study of lateral components of coupler forces. Lateral coupler forces are then combined with centripetal inertia calculations to determine quasi-static lateral forces, quasi-static vertical forces and quasi-static bogie lateral to vertical ratio, allowing the study of stringlining, buckling and wagon rollover risks. The approach taken allows for different rolling stock lengths, overhang and coupling lengths, and allows the study of angles occurring in transitions. Wagon body and bogie pitch are also studied with enhancements added to previous modelling to allow the study of wagon lift-off.

Instrumental record of debris flow initiation during natural rainfall: Implications for modeling slope stability

USGS Publications Warehouse

Montgomery, D.R.; Schmidt, K.M.; Dietrich, W.E.; McKean, J.

2009-01-01

The middle of a hillslope hollow in the Oregon Coast Range failed and mobilized as a debris flow during heavy rainfall in November 1996. Automated pressure transducers recorded high spatial variability of pore water pressure within the area that mobilized as a debris flow, which initiated where local upward flow from bedrock developed into overlying colluvium. Postfailure observations of the bedrock surface exposed in the debris flow scar reveal a strong spatial correspondence between elevated piezometric response and water discharging from bedrock fractures. Measurements of apparent root cohesion on the basal (Cb) and lateral (Cl) scarp demonstrate substantial local variability, with areally weighted values of Cb = 0.1 and Cl = 4.6 kPa. Using measured soil properties and basal root strength, the widely used infinite slope model, employed assuming slope parallel groundwater flow, provides a poor prediction of hydrologie conditions at failure. In contrast, a model including lateral root strength (but neglecting lateral frictional strength) gave a predicted critical value of relative soil saturation that fell within the range defined by the arithmetic and geometric mean values at the time of failure. The 3-D slope stability model CLARA-W, used with locally observed pore water pressure, predicted small areas with lower factors of safety within the overall slide mass at sites consistent with field observations of where the failure initiated. This highly variable and localized nature of small areas of high pore pressure that can trigger slope failure means, however, that substantial uncertainty appears inevitable for estimating hydrologie conditions within incipient debris flows under natural conditions. Copyright 2009 by the American Geophysical Union.

Influence of vortical flow structures on the glottal jet location in the supraglottal region.

PubMed

Kniesburges, Stefan; Hesselmann, Christina; Becker, Stefan; Schlücker, Eberhard; Döllinger, Michael

2013-09-01

Within the fully coupled multiphysics phonation process, the fluid flow plays an important role for sound production. This study addresses phenomena in the flow downstream of synthetic self-oscillating vocal folds. An experimental setup consisting of devices for producing and conditioning the flow including the main test channel was applied. The supraglottal channel was designed to prevent an acoustic coupling to the vocal folds. Hence, the oscillations were aerodynamically driven. The cross-section of the supraglottal channel was systematically varied by increasing the distance between the lateral channel walls. The vocal folds consisted of silicone rubber of homogenous material distribution generating self-sustained oscillations. The airflow was visualized in the immediate supraglottal region using a laser-sheet technique and a digital high-speed camera. Furthermore, the flow was studied by measuring the static pressure distributions on both lateral supraglottal channel walls. The results clearly showed different flow characteristics depending on the supraglottal configuration. In all cases with supraglottal channel, the jet was located asymmetrical and bent in medial-lateral direction. Furthermore, the side to which the jet was deflected changed in between the consecutive cycles showing a bifurcational behavior. Previously, this phenomenon was explained by the Coanda effect. However, the present data suggest that the deflection of the jet was mainly caused by large air vortices in the supraglottal channel produced by the flow field of previous oscillations. In contrast, for the case without supraglottal channel, the air jet was found totally symmetrical stabilized by the constant pressure in the ambient region. The emitted sound signal showed additional subharmonic tonal peaks for the asymmetric flow cases, which are characteristics for diplophonia. Copyright © 2013 The Voice Foundation. Published by Mosby, Inc. All rights reserved.

Social Smiling and Its Components in High-Risk Infant Siblings without Later ASD Symptomatology

ERIC Educational Resources Information Center

Nichols, Caitlin McMahon; Ibañez, Lisa V.; Foss-Feig, Jennifer H.; Stone, Wendy L.

2014-01-01

Impaired affective expression, including social smiling, is common in children with autism spectrum disorder (ASD), and may represent an early marker for ASD in their infant siblings (Sibs-ASD). Social smiling and its component behaviors (eye contact and non-social smiling) were examined at 15 months in Sibs-ASD who demonstrated later ASD…

ITS component specification. Appendix B, Input data flows for components

DOT National Transportation Integrated Search

1997-11-01

The objective of the Polaris Project is to define an Intelligent Transportation Systems (ITS) architecture for the state of Minnesota. This appendix defines the input data flows for each component of the Polaris Physical Architecture.

ITS component specification. Appendix C, Output data flows for components

DOT National Transportation Integrated Search

1997-01-01

The objective of the Polaris Project is to define an Intelligent Transportation Systems (ITS) architecture for the state of Minnesota. This appendix defines the output data flows for each component of the Polaris Physical Architecture.

The Landlab v1.0 OverlandFlow component: a Python tool for computing shallow-water flow across watersheds

NASA Astrophysics Data System (ADS)

Adams, Jordan M.; Gasparini, Nicole M.; Hobley, Daniel E. J.; Tucker, Gregory E.; Hutton, Eric W. H.; Nudurupati, Sai S.; Istanbulluoglu, Erkan

2017-04-01

Representation of flowing water in landscape evolution models (LEMs) is often simplified compared to hydrodynamic models, as LEMs make assumptions reducing physical complexity in favor of computational efficiency. The Landlab modeling framework can be used to bridge the divide between complex runoff models and more traditional LEMs, creating a new type of framework not commonly used in the geomorphology or hydrology communities. Landlab is a Python-language library that includes tools and process components that can be used to create models of Earth-surface dynamics over a range of temporal and spatial scales. The Landlab OverlandFlow component is based on a simplified inertial approximation of the shallow water equations, following the solution of de Almeida et al.(2012). This explicit two-dimensional hydrodynamic algorithm simulates a flood wave across a model domain, where water discharge and flow depth are calculated at all locations within a structured (raster) grid. Here, we illustrate how the OverlandFlow component contained within Landlab can be applied as a simplified event-based runoff model and how to couple the runoff model with an incision model operating on decadal timescales. Examples of flow routing on both real and synthetic landscapes are shown. Hydrographs from a single storm at multiple locations in the Spring Creek watershed, Colorado, USA, are illustrated, along with a map of shear stress applied on the land surface by flowing water. The OverlandFlow component can also be coupled with the Landlab DetachmentLtdErosion component to illustrate how the non-steady flow routing regime impacts incision across a watershed. The hydrograph and incision results are compared to simulations driven by steady-state runoff. Results from the coupled runoff and incision model indicate that runoff dynamics can impact landscape relief and channel concavity, suggesting that, on landscape evolution timescales, the OverlandFlow model may lead to differences in simulated topography in comparison with traditional methods. The exploratory test cases described within demonstrate how the OverlandFlow component can be used in both hydrologic and geomorphic applications.

Aptamer-phage reporters for ultrasensitive lateral flow assays

PubMed Central

Adhikari, Meena; Strych, Ulrich; Kim, Jinsu; Goux, Heather; Dhamane, Sagar; Poongavanam, Mohan-Vivekanandan; Hagström, Anna E. V.; Kourentzi, Katerina; Conrad, Jacinta C.; Willson, Richard C.

2015-01-01

We introduce the modification of bacteriophage particles with aptamers for the use as bioanalytical reporters, and demonstrate the use of these particles in ultrasensitive lateral flow assays. M13 phage displaying an in vivo biotinylatable peptide (AviTag) genetically fused to the phage tail protein pIII were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP) reporter enzymes covalently attached to the pVIII coat protein. These modified viral nanoparticles were used in immunochromatographic sandwich assays for the direct detection of IgE and of the penicillin-binding protein from Staphylococcus aureus (PBP2a). We also developed an additional lateral flow assay for IgE, in which the analyte is sandwiched between immobilized anti-IgE antibodies and aptamer-bearing reporter phage modified with HRP. The limit of detection of this LFA was 0.13 ng/mL IgE, ~100 times lower than those of previously reported IgE assays. PMID:26456715

Aptamer-Phage Reporters for Ultrasensitive Lateral Flow Assays.

PubMed

Adhikari, Meena; Strych, Ulrich; Kim, Jinsu; Goux, Heather; Dhamane, Sagar; Poongavanam, Mohan-Vivekanandan; Hagström, Anna E V; Kourentzi, Katerina; Conrad, Jacinta C; Willson, Richard C

2015-12-01

We introduce the modification of bacteriophage particles with aptamers for use as bioanalytical reporters, and demonstrate the use of these particles in ultrasensitive lateral flow assays. M13 phage displaying an in vivo biotinylatable peptide (AviTag) genetically fused to the phage tail protein pIII were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP) reporter enzymes covalently attached to the pVIII coat protein. These modified viral nanoparticles were used in immunochromatographic sandwich assays for the direct detection of IgE and of the penicillin-binding protein from Staphylococcus aureus (PBP2a). We also developed an additional lateral flow assay for IgE, in which the analyte is sandwiched between immobilized anti-IgE antibodies and aptamer-bearing reporter phage modified with HRP. The limit of detection of this LFA was 0.13 ng/mL IgE, ∼100 times lower than those of previously reported IgE assays.

Designing novel nano-immunoassays: antibody orientation versus sensitivity

NASA Astrophysics Data System (ADS)

Puertas, S.; Moros, M.; Fernández-Pacheco, R.; Ibarra, M. R.; Grazú, V.; de la Fuente, J. M.

2010-12-01

There is a growing interest in the use of magnetic nanoparticles (MNPs) for their application in quantitative and highly sensitive biosensors. Their use as labels of biological recognition events and their detection by means of some magnetic method constitute a very promising strategy for quantitative high-sensitive lateral-flow assays. In this paper, we report the importance of nanoparticle functionalization for the improvement of sensitivity for a lateral-flow immunoassay. More precisely, we have found that immobilization of IgG anti-hCG through its polysaccharide moieties on MNPs allows more successful recognition of the hCG hormone. Although we have used the detection of hCG as a model in this work, the strategy of binding antibodies to MNPs through its sugar chains reported here is applicable to other antibodies. It has huge potential as it will be very useful for the development of quantitative and high-sensitive lateral-flow assays for its use on human and veterinary, medicine, food and beverage manufacturing, pharmaceutical, medical biologics and personal care product production, environmental remediation, etc.

A novel multi-walled carbon nanotube-based antibody conjugate for quantitative and semi-quantitative lateral flow assays.

PubMed

Sun, Wenjuan; Hu, Xiaolong; Liu, Jia; Zhang, Yurong; Lu, Jianzhong; Zeng, Libo

2017-10-01

In this study, the multi-walled carbon nanotubes (MWCNTs) were applied in lateral flow strips (LFS) for semi-quantitative and quantitative assays. Firstly, the solubility of MWCNTs was improved using various surfactants to enhance their biocompatibility for practical application. The dispersed MWCNTs were conjugated with the methamphetamine (MET) antibody in a non-covalent manner and then manufactured into the LFS for the quantitative detection of MET. The MWCNTs-based lateral flow assay (MWCNTs-LFA) exhibited an excellent linear relationship between the values of test line and MET when its concentration ranges from 62.5 to 1500 ng/mL. The sensitivity of the LFS was evaluated by conjugating MWCNTs with HCG antibody and the MWCNTs conjugated method is 10 times more sensitive than the one conjugated with classical colloidal gold nanoparticles. Taken together, our data demonstrate that MWCNTs-LFA is a more sensitive and reliable assay for semi-quantitative and quantitative detection which can be used in forensic analysis.

A CCD-based reader combined with CdS quantum dot-labeled lateral flow strips for ultrasensitive quantitative detection of CagA

NASA Astrophysics Data System (ADS)

Gui, Chen; Wang, Kan; Li, Chao; Dai, Xuan; Cui, Daxiang

2014-02-01

Immunochromatographic assays are widely used to detect many analytes. CagA is proved to be associated closely with initiation of gastric carcinoma. Here, we reported that a charge-coupled device (CCD)-based test strip reader combined with CdS quantum dot-labeled lateral flow strips for quantitative detection of CagA was developed, which used 365-nm ultraviolet LED as the excitation light source, and captured the test strip images through an acquisition module. Then, the captured image was transferred to the computer and was processed by a software system. A revised weighted threshold histogram equalization (WTHE) image processing algorithm was applied to analyze the result. CdS quantum dot-labeled lateral flow strips for detection of CagA were prepared. One hundred sera samples from clinical patients with gastric cancer and healthy people were prepared for detection, which demonstrated that the device could realize rapid, stable, and point-of-care detection, with a sensitivity of 20 pg/mL.

Soil pipe flow tracer experiments: 1. Connectivity and transport characteristics

USDA-ARS?s Scientific Manuscript database

Much debate has occurred in catchment hydrology regarding the connectivity of flow paths from upslope areas to catchment outlets. This study was conducted in two catchments, one with three upper branches, in a loess soil with a fragipan that fosters lateral flow and exhibits an extensive distributio...

Shock Train/Boundary-Layer Interaction in Rectangular Scramjet Isolators

NASA Astrophysics Data System (ADS)

Geerts, Jonathan Simon

Numerous studies of the dual-mode scramjet isolator, a critical component in preventing inlet unstart and/or vehicle loss by containing a collection of flow disturbances called a shock train, have been performed since the dual-mode propulsion cycle was introduced in the 1960s. Low momentum corner flow and other three-dimensional effects inherent to rectangular isolators have, however, been largely ignored in experimental studies of the boundary layer separation driven isolator shock train dynamics. Furthermore, the use of two dimensional diagnostic techniques in past works, be it single-perspective line-of-sight schlieren/shadowgraphy or single axis wall pressure measurements, have been unable to resolve the three-dimensional flow features inside the rectangular isolator. These flow characteristics need to be thoroughly understood if robust dual-mode scramjet designs are to be fielded. The work presented in this thesis is focused on experimentally analyzing shock train/boundary layer interactions from multiple perspectives in aspect ratio 1.0, 3.0, and 6.0 rectangular isolators with inflow Mach numbers ranging from 2.4 to 2.7. Secondary steady-state Computational Fluid Dynamics studies are performed to compare to the experimental results and to provide additional perspectives of the flow field. Specific issues that remain unresolved after decades of isolator shock train studies that are addressed in this work include the three-dimensional formation of the isolator shock train front, the spatial and temporal low momentum corner flow separation scales, the transient behavior of shock train/boundary layer interaction at specific coordinates along the isolator's lateral axis, and effects of the rectangular geometry on semi-empirical relations for shock train length prediction. (Abstract shortened by ProQuest.).

Multiple Cross Displacement Amplification Coupled With Nanoparticles-Based Lateral Flow Biosensor for Detection of Staphylococcus aureus and Identification of Methicillin-Resistant S. aureus.

PubMed

Wang, Yi; Yan, Weiqiang; Fu, Shanshan; Hu, Shoukui; Wang, Yan; Xu, Jianguo; Ye, Changyun

2018-01-01

Staphylococcus aureus ( S. aureus ), including methicillin-resistant S. aureus (MRSA), is one of the most important human pathogens, which is responsible for bacteremia, soft-tissue infections, and food poisoning. Hence, multiple cross displacement amplification (MCDA) is employed to detect all S. aureus strains, and differentiates MRSA from methicillin-sensitive S. aureus . Multiplex MCDA (m-MCDA), which targets the nuc gene ( S. aureus -specific gene) and mecA gene (encoding penicillin-binding protein-2'), could detect S. aureus strains and identify MRSA within 85 min. Detection of the m-MCDA products is achieved using disposable lateral flow biosensors. A total of 58 strains, including various species of Gram-positive and Gram-negative strains, are used for evaluating and optimizing m-MCDA assays. The optimal amplification condition is found to be 63°C for 40 min, with detection limits at 100 fg DNA/reaction for nuc and mecA genes in the pure cultures, and 10 CFU/tube for nuc and mecA genes in the blood samples. The analytical specificity of m-MCDA assay is of 100%, and no cross-reactions to non- S. aureus strains are produced according to the specificity testing. Particularly, two additional components, including AUDG enzyme and dUTP, are added into the m-MCDA amplification mixtures, which are used for eliminating the unwanted results arising from carryover contamination. Thus, the m-MCDA technique appears to be a simple, rapid, sensitive, and reliable assay to detect all S. aureus strains, and identify MRSA infection for appropriate antibiotic therapy.

Blood coagulation screening using a paper-based microfluidic lateral flow device.

PubMed

Li, H; Han, D; Pauletti, G M; Steckl, A J

2014-10-21

A simple approach to the evaluation of blood coagulation using a microfluidic paper-based lateral flow assay (LFA) device for point-of-care (POC) and self-monitoring screening is reported. The device utilizes whole blood, without the need for prior separation of plasma from red blood cells (RBC). Experiments were performed using animal (rabbit) blood treated with trisodium citrate to prevent coagulation. CaCl2 solutions of varying concentrations are added to citrated blood, producing Ca(2+) ions to re-establish the coagulation cascade and mimic different blood coagulation abilities in vitro. Blood samples are dispensed into a paper-based LFA device consisting of sample pad, analytical membrane and wicking pad. The porous nature of the cellulose membrane separates the aqueous plasma component from the large blood cells. Since the viscosity of blood changes with its coagulation ability, the distance RBCs travel in the membrane in a given time can be related to the blood clotting time. The distance of the RBC front is found to decrease linearly with increasing CaCl2 concentration, with a travel rate decreasing from 3.25 mm min(-1) for no added CaCl2 to 2.2 mm min(-1) for 500 mM solution. Compared to conventional plasma clotting analyzers, the LFA device is much simpler and it provides a significantly larger linear range of measurement. Using the red colour of RBCs as a visible marker, this approach can be utilized to produce a simple and clear indicator of whether the blood condition is within the appropriate range for the patient's condition.

High-resolution, preparative purification of PEGylated protein using a laterally-fed membrane chromatography device.

PubMed

Madadkar, Pedram; Nino, Sergio Luna; Ghosh, Raja

2016-11-01

We discuss the use of a laterally-fed membrane chromatography (or LFMC) device for single-step purification of mono-PEGylated lysozyme. Recent studies have shown such LFMC devices to be suitable for high-resolution, multi-component separation of proteins in the bind-and-elute mode. The device used in this study contained a stack of rectangular cation-exchange membranes having 9.25mL bed volume. PEGylation of lysozyme was carried out in batch mode using 5kDa methoxy-polyethyleneglycol propionaldehyde (or m-PEG propionaldehyde) in the presence of sodium cyanoborohydride as reducing agent. Membrane chromatographic separation was carried out at 1.62 membrane bed volumes per minute flow rate, in the bind-and-elute mode. When a salt gradient was applied, the higher PEGylated forms of lysozyme (i.e. the byproducts) eluted earlier than mono-PEGylated lysozyme (the target product), while lysozyme eluted last. Under elution conditions optimized for resolution and speed, the separation could be carried out in less than 15 membrane bed volumes. High purity and recovery of mono-PEGylated lysozyme was obtained. The resolution of separation of mono-PEGylated lysozyme obtained under the above condition was comparable to that reported in the literature for equivalent cation-exchange resin columns while the flow rate expressed in bed volumes/min was 21.7 times higher. Also, the number of theoretical plates per meter was significantly higher with the LFMC device. Therefore the LFMC based purification process discussed in this paper combined high-productivity with high-resolution. Copyright © 2016 Elsevier B.V. All rights reserved.

Wind modeling and lateral control for automatic landing

NASA Technical Reports Server (NTRS)

Holley, W. E.; Bryson, A. E., Jr.

1975-01-01

For the purposes of aircraft control system design and analysis, the wind can be characterized by a mean component which varies with height and by turbulent components which are described by the von Karman correlation model. The aircraft aero-dynamic forces and moments depend linearly on uniform and gradient gust components obtained by averaging over the aircraft's length and span. The correlations of the averaged components are then approximated by the outputs of linear shaping filters forced by white noise. The resulting model of the crosswind shear and turbulence effects is used in the design of a lateral control system for the automatic landing of a DC-8 aircraft.

Evaluation of detection methods for screening meat and poultry products for the presence of foodborne pathogens.

PubMed

Bohaychuk, Valerie M; Gensler, Gary E; King, Robin K; Wu, John T; McMullen, Lynn M

2005-12-01

Rapid and molecular technologies such as enzyme-linked immunosorbent assay (ELISA), PCR, and lateral flow immunoprecipitation can reduce the time and labor involved in screening food products for the presence of pathogens. These technologies were compared with conventional culture methodology for the detection of Salmonella, Campylobacter, Listeria, and Escherichia coli O157:H7 inoculated in raw and processed meat and poultry products. Recommended protocols were modified so that the same enrichment broths used in the culture methods were also used in the ELISA, PCR, and lateral flow immunoprecipitation assays. The percent agreement between the rapid technologies and culture methods ranged from 80 to 100% depending on the pathogen detected and the method used. ELISA, PCR, and lateral flow immunoprecipitation all performed well, with no statistical difference, compared with the culture method for the detection of E. coli O157:H7. ELISA performed better for the detection of Salmonella, with sensitivity and specificity rates of 100%. PCR performed better for the detection of Campylobacter jejuni, with 100% agreement to the culture method. PCR was highly sensitive for the detection of all the foodborne pathogens tested except Listeria monocytogenes. Although the lateral flow immunoprecipitation tests were statistically different from the culture methods for Salmonella and Listeria because of false-positive results, the tests did not produce any false negatives, indicating that this method would be suitable for screening meat and poultry products for these pathogens.

Three-dimensional inspiratory flow in a double bifurcation airway model

NASA Astrophysics Data System (ADS)

Jalal, Sahar; Nemes, Andras; Van de Moortele, Tristan; Schmitter, Sebastian; Coletti, Filippo

2016-09-01

The flow in an idealized airway model is investigated for the steady inhalation case. The geometry consists of a symmetric planar double bifurcation that reflects the anatomical proportions of the human bronchial tree, and a wide range of physiologically relevant Reynolds numbers ( Re = 100-5000) is considered. Using magnetic resonance velocimetry, we analyze the three-dimensional fields of velocity and vorticity, along with flow descriptors that characterize the longitudinal and lateral dispersion. In agreement with previous studies, the symmetry of the flow partitioning is broken even at the lower Reynolds numbers, and at the second bifurcation, the fluid favors the medial branches over the lateral ones. This trend reaches a plateau around Re = 2000, above which the turbulent inflow results in smoothed mean velocity gradients. This also reduces the streamwise momentum flux, which is a measure of the longitudinal dispersion by the mean flow. The classic Dean-type counter-rotating vortices are observed in the first-generation daughter branches as a result of the local curvature. In the granddaughter branches, however, the secondary flows are determined by the local curvature only for the lower flow regimes ( Re ≤ 250), in which case the classic Dean mechanism prevails. At higher flow regimes, the field is instead dominated by streamwise vortices extending from the daughter into the medial granddaughter branches, where they rotate in the opposite direction with respect to Dean vortices. Circulation and secondary flow intensity show a similar trend as the momentum flux, increasing with Reynolds number up to Re = 2000 and then dropping due to turbulent dissipation of vorticity. The streamwise vortices interact both with each other and with the airway walls, and for Re > 500 they can become stronger in the medial granddaughter than in the upstream daughter branches. With respect to realistic airway models, the idealized geometry produces weaker secondary flows, suggesting that realistic anatomical features may generate more lateral dispersion than canonical symmetric models.

Dual-RiverSonde measurements of two-dimensional river flow patterns

USGS Publications Warehouse

Teague, C.C.; Barrick, D.E.; Lilleboe, P.M.; Cheng, R.T.; Stumpner, P.; Burau, J.R.

2008-01-01

Two-dimensional river flow patterns have been measured using a pair of RiverSondes in two experiments in the Sacramento-San Joaquin River Delta system of central California during April and October 2007. An experiment was conducted at Walnut Grove, California in order to explore the use of dual RiverSondes to measure flow patterns at a location which is important in the study of juvenile fish migration. The data available during the first experiment were limited by low wind, so a second experiment was conducted at Threemile Slough where wind conditions and surface turbulence historically have resulted in abundant data. Both experiments included ADCP near-surface velocity measurements from either manned or unmanned boats. Both experiments showed good comparisons between the RiverSonde and ADCP measurements. The flow conditions at both locations are dominated by tidal effects, with partial flow reversal at Walnut Grove and complete flow reversal at Threemile Slough. Both systems showed complex flow patterns during the flow reversals. Quantitative comparisons between the RiverSondes and an ADCP on a manned boat at Walnut Grove showed mean differences of 4.5 cm/s in the u (eastward) and 7.6 cm/s in the v (northward) components, and RMS differences of 14.7 cm/s in the u component and 21.0 cm/s in the v component. Quantitative comparisons between the RiverSondes and ADCPs on autonomous survey vessels at Threemile Slough showed mean differences of 0.007 cm/s in the u component and 0.5 cm/s in the v component, and RMS differences of 7.9 cm/s in the u component and 13.5 cm/s in the v component after obvious outliers were removed. ?? 2008 IEEE.

A grid-embedding transonic flow analysis computer program for wing/nacelle configurations

NASA Technical Reports Server (NTRS)

Atta, E. H.; Vadyak, J.

1983-01-01

An efficient grid-interfacing zonal algorithm was developed for computing the three-dimensional transonic flow field about wing/nacelle configurations. the algorithm uses the full-potential formulation and the AF2 approximate factorization scheme. The flow field solution is computed using a component-adaptive grid approach in which separate grids are employed for the individual components in the multi-component configuration, where each component grid is optimized for a particular geometry such as the wing or nacelle. The wing and nacelle component grids are allowed to overlap, and flow field information is transmitted from one grid to another through the overlap region using trivariate interpolation. This report represents a discussion of the computational methods used to generate both the wing and nacelle component grids, the technique used to interface the component grids, and the method used to obtain the inviscid flow solution. Computed results and correlations with experiment are presented. also presented are discussions on the organization of the wing grid generation (GRGEN3) and nacelle grid generation (NGRIDA) computer programs, the grid interface (LK) computer program, and the wing/nacelle flow solution (TWN) computer program. Descriptions of the respective subroutines, definitions of the required input parameters, a discussion on interpretation of the output, and the sample cases illustrating application of the analysis are provided for each of the four computer programs.

Hydrocephalus secondary to obstruction of the lateral apertures in two dogs.

PubMed

Kent, M; Glass, E N; Haley, A C; Shaikh, L S; Sequel, M; Blas-Machado, U; Bishop, T M; Holmes, S P; Platt, S R

2016-11-01

Traditionally, hydrocephalus is divided into communicating or non-communicating (obstructive) based on the identification of a blockage of cerebrospinal fluid (CSF) flow through the ventricular system. Hydrocephalus ex vacuo refers to ventricular enlargement as a consequence of neuroparenchymal loss. Hydrocephalus related to obstruction of the lateral apertures of the fourth ventricles has rarely been described. The clinicopathologic findings in two dogs with hydrocephalus secondary to obstruction of the lateral apertures of the fourth ventricle are reported. Signs were associated with a caudal cervical spinal cord lesion in one dog and a caudal brain stem lesion in the other dog. Magnetic resonance imaging (MRI) disclosed dilation of the ventricular system, including the lateral recesses of the fourth ventricle. In one dog, postmortem ventriculography confirmed obstruction of the lateral apertures. Microscopic changes were identified in the choroid plexus in both dogs, yet a definitive cause of the obstructions was not identified. The MRI findings in both dogs are similar to membranous occlusion of the lateral and median apertures in human patients. MRI detection of dilation of the entire ventricular system in the absence of an identifiable cause should prompt consideration of an obstruction of the lateral apertures. In future cases, therapeutic interventions aimed at re-establishing CSF flow or ventriculoperitoneal catheterisation should be considered. © 2016 Australian Veterinary Association.

Geology and Conceptual Model of the Domuyo Geothermal Area, Patagonia, Argentina

NASA Astrophysics Data System (ADS)

Fragoso, A. S.; Ferrari, L.; Norini, G.

2017-12-01

Cerro Domuyo is the highest mountain in Patagonia and its western slope is characterized by thermal springs with boiling fluids as well as silicic domes and pyroclastic deposits that suggest the existence of a geothermal reservoir. Early studies proposed that the thermal springs were fault-controlled and the reservoir was located in a graben bounded by E-W normal faults. A recent geochemical study estimated a temperature of 220ºC for the fluid reservoir and a thermal energy release of 1.1 GW, one of the world largest advective heat flux from a continental volcanic center. We carried out a geologic survey and U-Pb and U-Th geochronologic study to elaborate an updated conceptual model for the Domuyo geothermal area. Our study indicates that the Domuyo Volcanic Complex (DVC) is a dome complex overlying an older, Middle Miocene to Pliocene volcanic sequence widely exposed to the southwest and to the north, which in turn covers: 1) the Jurassice-Early Creteacoeus Neuquen marine sedimentary succession, 2) silicic ignimbrites dated at 186.7 Ma and, 3) the Paleozoic metamorphic basement intruded by 288 Ma granite bodies. These pre-Cenozoic successions are involved in dominantly N-S trending folds and thrust faults later displaced by E-W striking normal faults with a right lateral component of motion that underlie the DVC. The volcanic cycle forming the DVC is distinctly bimodal with the emplacement of massive silicic domes but also less voluminous olivine basalts on its southern slope. The central dome underwent a major collapse that produced 0.35 km3 of ash and block flow and associated pyroclastic flows that filled the valley to the southwest up to 30 km from the source. This was followed by a voluminous effusive activity that formed silicic domes dated between 254-322 Ky, which is inferred to overlain a partially molten silicic magma chamber. Integrating the geologic model with magnetotelluric and gravity surveys we developed a conceptual model of the geothermal system in which the reservoir is inferred at a depth of less than 2 km in pre-Pliocene fractured rocks, bounded by E-W faults and sealed by the pyroclastic deposits and rhyolitic lavas of the DVC. The location of most thermal springs is not controlled by faults. Rather, they are lateral flows emerging at the contact between the fractured basement and the caprock.

Robots for Aircraft Maintenance

NASA Technical Reports Server (NTRS)

1993-01-01

Marshall Space Flight Center charged USBI (now Pratt & Whitney) with the task of developing an advanced stripping system based on hydroblasting to strip paint and thermal protection material from Space Shuttle solid rocket boosters. A robot, mounted on a transportable platform, controls the waterjet angle, water pressure and flow rate. This technology, now known as ARMS, has found commercial applications in the removal of coatings from jet engine components. The system is significantly faster than manual procedures and uses only minimal labor. Because the amount of "substrate" lost is minimal, the life of the component is extended. The need for toxic chemicals is reduced, as is waste disposal and human protection equipment. Users of the ARMS work cell include Delta Air Lines and the Air Force, which later contracted with USBI for development of a Large Aircraft Paint Stripping system (LARPS). LARPS' advantages are similar to ARMS, and it has enormous potential in military and civil aircraft maintenance. The technology may also be adapted to aircraft painting, aircraft inspection techniques and paint stripping of large objects like ships and railcars.

DOE-FG02-00ER62797 Final Report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sweedler, J.V.

2004-12-01

Specific Aims The overall goal of this proposal has been to develop and interface a new technology, molecular gates, with microfabricated systems to add an important capability to microfabricated DNA measurement systems. This project specifically focused on demonstrating how molecular gates could be used to capture a single analyte band, among a stream of bands from a separation or a flow injection analysis experiment, and release it for later measurement, thus allowing further manipulations on the selected analyte. Since the original proposal, the molecular gate concept has been greatly expanded to allow the gates to be used as externally controllablemore » intelligent interconnects in multilayer microfluidic networks. We have demonstrated: (1) the ability of the molecular gates to work with a much wider range of biological molecules including DNA, proteins and small metabolites; and (2) the capability of performing an electrophoretic separation and sequestering individual picoliter volume components (or even classes of components) into separate channels for further analysis. Both capabilities will enable characterization of small mass amounts of complex mixtures of DNA, proteins and even small molecules--allowing them to be further separated and chemically characterized.« less

Dynamic Analysis of a Rotor System Supported on Squeeze Film Damper with Air Entrainment

NASA Astrophysics Data System (ADS)

Zhang, Wei; Han, Bingbing; Zhang, Kunpeng; Ding, Qian

2017-12-01

Squeeze film dampers (SFDs) are widely used in compressors and turbines to suppress the vibration while traversing critical speeds. In practical applications, air ingestion from the outside environment and cavitation may lead to a foamy lubricant that weakens oil film damping and dynamic performance of rotor system. In this paper, a rigid rotor model is established considering both lateral and pitching vibration under different imbalance excitations to evaluate the effect of air entrainment on rotor system. Tests with three different imbalances are carried out on a rotor-SFD apparatus. Volume controlled air in mixture ranging from pure oil to all air are supplied to the SFD. The transient response of rotor is measured in the experiments. The results show that two-phase flow produces significant influence on the system stability and dynamical response. The damping properties are weakened by entrained air, such as the damping on high frequency components of rolling ball bearing. Super-harmonic resonance and bifurcation are observed, as well as the low frequency components due to air entrainment.

Earth-strength magnetic field affects the rheotactic threshold of zebrafish swimming in shoals.

PubMed

Cresci, Alessandro; De Rosa, Rosario; Putman, Nathan F; Agnisola, Claudio

2017-02-01

Rheotaxis, the unconditioned orienting response to water currents, is a main component of fish behavior. Rheotaxis is achieved using multiple sensory systems, including visual and tactile cues. Rheotactic orientation in open or low-visibility waters might also benefit from the stable frame of reference provided by the geomagnetic field, but this possibility has not been explored before. Zebrafish (Danio rerio) form shoals living in freshwater systems with low visibility, show a robust positive rheotaxis, and respond to geomagnetic fields. Here, we investigated whether a static magnetic field in the Earth-strength range influenced the rheotactic threshold of zebrafish in a swimming tunnel. The direction of the horizontal component of the magnetic field relative to water flow influenced the rheotactic threshold of fish as part of a shoal, but not of fish tested alone. Results obtained after disabling the lateral line of shoaling individuals with Co 2+ suggest that this organ system is involved in the observed magneto-rheotactic response. These findings constitute preliminary evidence that magnetic fields influence rheotaxis and suggest new avenues for further research. Copyright © 2016 Elsevier Inc. All rights reserved.

Global Qualitative Flow-Path Modeling for Local State Determination in Simulation and Analysis

NASA Technical Reports Server (NTRS)

Malin, Jane T. (Inventor); Fleming, Land D. (Inventor)

1998-01-01

For qualitative modeling and analysis, a general qualitative abstraction of power transmission variables (flow and effort) for elements of flow paths includes information on resistance, net flow, permissible directions of flow, and qualitative potential is discussed. Each type of component model has flow-related variables and an associated internal flow map, connected into an overall flow network of the system. For storage devices, the implicit power transfer to the environment is represented by "virtual" circuits that include an environmental junction. A heterogeneous aggregation method simplifies the path structure. A method determines global flow-path changes during dynamic simulation and analysis, and identifies corresponding local flow state changes that are effects of global configuration changes. Flow-path determination is triggered by any change in a flow-related device variable in a simulation or analysis. Components (path elements) that may be affected are identified, and flow-related attributes favoring flow in the two possible directions are collected for each of them. Next, flow-related attributes are determined for each affected path element, based on possibly conflicting indications of flow direction. Spurious qualitative ambiguities are minimized by using relative magnitudes and permissible directions of flow, and by favoring flow sources over effort sources when comparing flow tendencies. The results are output to local flow states of affected components.

Local Modelling of Groundwater Flow Using Analytic Element Method Three-dimensional Transient Unconfined Groundwater Flow With Partially Penetrating Wells and Ellipsoidal Inhomogeneites

NASA Astrophysics Data System (ADS)

Jankovic, I.; Barnes, R. J.; Soule, R.

2001-12-01

The analytic element method is used to model local three-dimensional flow in the vicinity of partially penetrating wells. The flow domain is bounded by an impermeable horizontal base, a phreatic surface with recharge and a cylindrical lateral boundary. The analytic element solution for this problem contains (1) a fictitious source technique to satisfy the head and the discharge conditions along the phreatic surface, (2) a fictitious source technique to satisfy specified head conditions along the cylindrical boundary, (3) a method of imaging to satisfy the no-flow condition across the impermeable base, (4) the classical analytic solution for a well and (5) spheroidal harmonics to account for the influence of the inhomogeneities in hydraulic conductivity. Temporal variations of the flow system due to time-dependent recharge and pumping are represented by combining the analytic element method with a finite difference method: analytic element method is used to represent spatial changes in head and discharge, while the finite difference method represents temporal variations. The solution provides a very detailed description of local groundwater flow with an arbitrary number of wells of any orientation and an arbitrary number of ellipsoidal inhomogeneities of any size and conductivity. These inhomogeneities may be used to model local hydrogeologic features (such as gravel packs and clay lenses) that significantly influence the flow in the vicinity of partially penetrating wells. Several options for specifying head values along the lateral domain boundary are available. These options allow for inclusion of the model into steady and transient regional groundwater models. The head values along the lateral domain boundary may be specified directly (as time series). The head values along the lateral boundary may also be assigned by specifying the water-table gradient and a head value at a single point (as time series). A case study is included to demonstrate the application of the model in local modeling of the groundwater flow. Transient three-dimensional capture zones are delineated for a site on Prairie Island, MN. Prairie Island is located on the Mississippi River 40 miles south of the Twin Cities metropolitan area. The case study focuses on a well that has been known to contain viral DNA. The objective of the study was to assess the potential for pathogen migration toward the well.

Cardiovascular autonomic modulation and activity of carotid baroreceptors at altitude.

PubMed

Bernardi, L; Passino, C; Spadacini, G; Calciati, A; Robergs, R; Greene, R; Martignoni, E; Anand, I; Appenzeller, O

1998-11-01

1. To assess the effects of acute exposure to high altitude on baroreceptor function in man we evaluated the effects of baroreceptor activation on R-R interval and blood pressure control at high altitude. We measured the low-frequency (LF) and high-frequency (HF) components in R-R, non-invasive blood pressure and skin blood flow, and the effect of baroreceptor modulation by 0. 1-Hz sinusoidal neck suction. Ten healthy sea-level natives and three high-altitude native, long-term sea-level residents were evaluated at sea level, upon arrival at 4970 m and 1 week later.2. Compared with sea level, acute high altitude decreased R-R and increased blood pressure in all subjects [sea-level natives: R-R from 1002+/-45 to 775+/-57 ms, systolic blood pressure from 130+/-3 to 150+/-8 mmHg; high-altitude natives: R-R from 809+/-116 to 749+/-47 ms, systolic blood pressure from 110+/-12 to 125+/-11 mmHg (P<0.05 for all)]. One week later systolic blood pressure was similar to values at sea level in all subjects, whereas R-R remained elevated in sea-level natives. The low-frequency power in R-R and systolic blood pressure increased in sea-level natives [R-R-LF from 47+/-8 to 65+/-10% (P<0.05), systolic blood pressure-LF from 1.7+/-0. 3 to 2.6+/-0.4 ln-mmHg2 (P<0.05)], but not in high-altitude natives (R-R-LF from 32+/-13 to 38+/-19%, systolic blood pressure-LF from 1. 9+/-0.5 to 1.7+/-0.8 ln-mmHg2). The R-R-HF decreased in sea-level natives but not in high-altitude natives, and no changes occurred in systolic blood pressure-HF. These changes remained evident 1 week later. Skin blood flow variability and its spectral components decreased markedly at high altitude in sea-level natives but showed no changes in high-altitude natives. Neck suction significantly increased the R-R- and systolic blood pressure-LF in all subjects at both sea level and high altitude.3. High altitude induces sympathetic activation in sea-level natives which is partially counteracted by active baroreflex. Despite long-term acclimatization at sea level, high-altitude natives also maintain active baroreflex at high altitude but with lower sympathetic activation, indicating a persisting high-altitude adaptation which may be genetic or due to baroreflex activity not completely lost by at least 1 year's sea-level residence.

Low pressure drop, multi-slit virtual impactor

DOEpatents

Bergman, Werner

2002-01-01

Fluid flow is directed into a multiplicity of slit nozzles positioned so that the fluid flow is directed into a gap between the nozzles and (a) a number of receiving chambers and (b) a number of exhaust chambers. The nozzles and chambers are select so that the fluid flow will be separated into a first particle flow component with larger and a second particle flow component with the smaller particles.

Multi-Component, Multi-Point Interferometric Rayleigh/Mie Doppler Velocimeter

NASA Technical Reports Server (NTRS)

Danehy, Paul M.; Lee, Joseph W.; Bivolaru, Daniel

2012-01-01

An interferometric Rayleigh scattering system was developed to enable the measurement of multiple, orthogonal velocity components at several points within very-high-speed or high-temperature flows. The velocity of a gaseous flow can be optically measured by sending laser light into the gas flow, and then measuring the scattered light signal that is returned from matter within the flow. Scattering can arise from either gas molecules within the flow itself, known as Rayleigh scattering, or from particles within the flow, known as Mie scattering. Measuring Mie scattering is the basis of all commercial laser Doppler and particle imaging velocimetry systems, but particle seeding is problematic when measuring high-speed and high-temperature flows. The velocimeter is designed to measure the Doppler shift from only Rayleigh scattering, and does not require, but can also measure, particles within the flow. The system combines a direct-view, large-optic interferometric setup that calculates the Doppler shift from fringe patterns collected with a digital camera, and a subsystem to capture and re-circulate scattered light to maximize signal density. By measuring two orthogonal components of the velocity at multiple positions in the flow volume, the accuracy and usefulness of the flow measurement increase significantly over single or nonorthogonal component approaches.

Gravity response mechanisms of lateral organs and the control of plant architecture in Arabidopsis

NASA Astrophysics Data System (ADS)

Mullen, J.; Hangarter, R.

Most research on gravity responses in plants has focused on primary roots and shoots, which typically grow in a vertical orientation. However, the patterns of lateral organ formation and their growth orientation, which typically are not vertical, govern plant architecture. For example, in Arabidopsis, when lateral roots emerge from the primary root, they grow at a nearly horizontal orientation. As they elongate, the roots slowly curve until they eventually reach a vertical orientation. The regulation of this lateral root orientation is an important component affecting the overall root system architecture. We have found that this change in orientation is not simply due to the onset of gravitropic competence, as non-vertical lateral roots are capable of both positive and negative gravitropism. Thus, the horizontal growth of the new lateral roots is determined by what is called the gravitropic set-point angle (GSA). In Arabidopsis shoots, rosette leaves and inflorescence branches also display GSA-dependent developmental changes in their orientation. The developmental control of the GSA of lateral organs in Arabidopsis provides us with a useful system for investigating the components involved in regulating directionality of tropistic responses. We have identified several Arabidopsis mutants that have either altered lateral root orientations, altered orientation of lateral organs in the shoot, or both, but maintain normal primary organ orientation. The mgsa ({m}odified {g}ravitropic {s}et-point {a}ngle) mutants with both altered lateral root and shoot orientation show that there are common components in the regulation of growth orientation in the different organs. Rosette leaves and lateral roots also have in common a regulation of positioning by red light. Further molecular and physiological analyses of the GSA mutants will provide insight into the basis of GSA regulation and, thus, a better understanding of how gravity controls plant architecture. [This work was supported by the National Aeronautics and Space Administration through grant no. NCC 2-1200.

An optofluidic metasurface for lateral flow-through detection of breast cancer biomarker.

PubMed

Wang, Yifei; Ali, Md Azahar; Chow, Edmond K C; Dong, Liang; Lu, Meng

2018-06-01

The rapid growth of point-of-care tests demands for biosensors with high sensitivity and small size. This paper demonstrates an optofluidic metasurface that combines silicon-on-insulator (SOI) nanophotonics and nanofluidics to realize a high-performance, lateral flow-through biosensor. The metasurface is made of a periodic array of silicon nanoposts on an SOI substrate, and functionalized with specific receptor molecules. Bonding of a polydimethylsiloxane slab directly onto the surface results in an ultracompact biosensor, where analyte solutions are restricted to flow only in the space between the nanoposts. No flow exists above the nanoposts. This sensor design overcomes the issue with diffusion-limited detection of many other biosensors. The lateral flow-through feature, in conjunction with high-Q resonance modes associated with optical bound states of the metasurface, offers an improved sensitivity to subtle molecule-bonding induced changes in refractive index. The device exhibits a resonance mode around 1550 nm wavelength and provides an index sensitivity of 720 nm/RIU. Biosensing is conducted to detect the epidermal growth factor receptor 2 (ErbB2), a protein biomarker for early-stage breast cancer screening, by monitoring resonance wavelength shifts in response to specific analyte-ligand binding events at the metasurface. The limit of detection of the device is 0.7 ng mL -1 for ErbB2. Copyright © 2018 Elsevier B.V. All rights reserved.

Randomized prospective study comparing tri-cortical iliac crest autograft to allograft in the lateral column lengthening component for operative correction of adult acquired flatfoot deformity.

PubMed

Dolan, Christopher M; Henning, Jeffrey A; Anderson, John G; Bohay, Donald R; Kornmesser, Marc J; Endres, Terrence J

2007-01-01

Operative treatment of stage II posterior tibial tendon insufficiency (PTTI) is controversial. Many soft-tissue and bony procedures and various combinations of the two have been reported for treatment of stage II PTTI. Orthopaedists recognize the lateral column lengthening component of the procedure as a successful reconstructive technique. The use of cortical allograft for lateral column lengthening in the correction of pes planus in the pediatric patient population has been routine. In the adult population, however, tricortical iliac crest autograft has been the bone graft of choice. Harvest of this autograft can precipitate significant morbidity and cost. Therefore, we undertook this randomized controlled trial to compare graft incorporation and healing of allograft and autograft in the lateral column lengthening component of adult flatfoot reconstruction. Lateral column lengthening was done as a component of operative correction for stage II PTTI in adult patients (older than 18 years) by two surgeons using similar procedures. The patients were randomized to either the allograft or autograft procedures. The primary endpoint was graft incorporation and healing as assessed by radiographs. The study included 33 randomized feet in 31 patients. We followed 18 feet in the allograft group and 15 in the autograft group to the point of union. There were 21 women and 10 men. There were no delayed unions, nonunions, or hardware failures. All patients in both groups achieved bony union by the 12-week followup evaluation. Two superficial foot infections were successfully treated with oral antibiotics. Two patients in the autograft group continued to have hip donor site pain at 3 months. This study suggests that union rates of allograft and autograft (iliac crest bone graft) are equal. The use of allograft in the lateral column lengthening component of operative correction of adult stage II PTTI appears to be a viable alternative to the use of iliac crest autograft and eliminates the morbidity and increased cost associated with autograft harvest.

Tutorial on Quantification of Differences between Single- and Two-Component Two-Phase Flow and Heat Transfer

NASA Astrophysics Data System (ADS)

Delil, A. A. M.

2003-01-01

Single-component two-phase systems are envisaged for aerospace thermal control applications: Mechanically Pumped Loops, Vapour Pressure Driven Loops, Capillary Pumped Loops and Loop Heat Pipes. Thermal control applications are foreseen in different gravity environments: Micro-g, reduced-g for Mars or Moon bases, 1-g during terrestrial testing, and hyper-g in rotating spacecraft, during combat aircraft manoeuvres and in systems for outer planets. In the evaporator, adiabatic line and condenser sections of such single-component two-phase systems, the fluid is a mixture of the working liquid (for example ammonia, carbon dioxide, ethanol, or other refrigerants, etc.) and its saturated vapour. Results of two-phase two-component flow and heat transfer research (pertaining to liquid-gas mixtures, e.g. water/air, or argon or helium) are often applied to support research on flow and heat transfer in two-phase single-component systems. The first part of the tutorial updates the contents of two earlier tutorials, discussing various aerospace-related two-phase flow and heat transfer research. It deals with the different pressure gradient constituents of the total pressure gradient, with flow regime mapping (including evaporating and condensing flow trajectories in the flow pattern maps), with adiabatic flow and flashing, and with thermal-gravitational scaling issues. The remaining part of the tutorial qualitatively and quantitatively determines the differences between single- and two-component systems: Two systems that physically look similar and close, but in essence are fully different. It was already elucidated earlier that, though there is a certain degree of commonality, the differences will be anything but negligible, in many cases. These differences (quantified by some examples) illustrates how careful one shall be in interpreting data resulting from two-phase two-component simulations or experiments, for the development of single-component two-phase thermal control systems for various gravity environments.

Flow pumping system for physiological waveforms.

PubMed

Tsai, William; Savaş, Omer

2010-02-01

A pulsatile flow pumping system is developed to replicate flow waveforms with reasonable accuracy for experiments simulating physiological blood flows at numerous points in the body. The system divides the task of flow waveform generation between two pumps: a gear pump generates the mean component and a piston pump generates the oscillatory component. The system is driven by two programmable servo controllers. The frequency response of the system is used to characterize its operation. The system has been successfully tested in vascular flow experiments where sinusoidal, carotid, and coronary flow waveforms are replicated.

Extension of a System Level Tool for Component Level Analysis

NASA Technical Reports Server (NTRS)

Majumdar, Alok; Schallhorn, Paul

2002-01-01

This paper presents an extension of a numerical algorithm for network flow analysis code to perform multi-dimensional flow calculation. The one dimensional momentum equation in network flow analysis code has been extended to include momentum transport due to shear stress and transverse component of velocity. Both laminar and turbulent flows are considered. Turbulence is represented by Prandtl's mixing length hypothesis. Three classical examples (Poiseuille flow, Couette flow and shear driven flow in a rectangular cavity) are presented as benchmark for the verification of the numerical scheme.

Extension of a System Level Tool for Component Level Analysis

NASA Technical Reports Server (NTRS)

Majumdar, Alok; Schallhorn, Paul; McConnaughey, Paul K. (Technical Monitor)

2001-01-01

This paper presents an extension of a numerical algorithm for network flow analysis code to perform multi-dimensional flow calculation. The one dimensional momentum equation in network flow analysis code has been extended to include momentum transport due to shear stress and transverse component of velocity. Both laminar and turbulent flows are considered. Turbulence is represented by Prandtl's mixing length hypothesis. Three classical examples (Poiseuille flow, Couette flow, and shear driven flow in a rectangular cavity) are presented as benchmark for the verification of the numerical scheme.

Building the Pamir-Tibet Plateau: Eo-Oligocene Crustal Stacking and Orogen Parallel Evasion of Upper and Middle Crustal Material in the Pamir

NASA Astrophysics Data System (ADS)

Rutte, D.; Ratschbacher, L.; Stübner, K.; Schneider, S.

2015-12-01

The gneisses of the Central Pamir Domes and their cover document crustal stacking of a ~10 km thick Ediacaran-Paleogene succession to a thickness of >35 km and their exhumation along bi-vergent, top-to-N and top-to S, normal-sense shear zones. The giant South Pamir Shakhdara-Alichur gneiss-dome system formed similarly by N-S extension along bivergent detachments. Prograde amphibolite-facies metamorphism in the domes and low-grade metamorphism in their hanging wall is dated at ~40 Ma (Lu-Hf garnet, U-Pb titanite) [Smit et al., 2014; Stearns et al., 2015] and ~33 Ma (K/Ar sericite). Retrograde metamorphism―driven by crustal extension―started at ~21 Ma (multi-method thermochronology; Stearns et al.[2013]). These Gneiss Domes offer a unique window into the Eocene-Miocene state of the Asian middle crust of the Pamir-Tibet Plateau. Top-to-N thrust stacking accommodated thickening in the upper crust, with displacements of single thrust sheets of > 30 and > 19 km. At depth, ductile flow formed km-scale recumbent fold nappes. We reconstruct their geometry by structural mapping and U-Pb zircon dating, documenting repetition of metatuffite, and paragneiss layers. In the interior of the domes, amphibolite-facies deformation fabrics with prograde kyanite define an E-W stretching lineation. Associated microstructures indicate top-to-E and top-to-W shear senses. Chocolate tablet boudinage indicate vertical flattening during bulk crustal thickening. We suggest that prograde E-W stretching relates to an early orogen-parallel flow component in the middle crust, contemporaneous with crustal stacking during bulk top-to-N convergence prior to ~21 Ma. Material likely evaded laterally out of the Pamir, contributing to >60 km thick crust in the Hindu Kush, west of the India-Asia frontal collision. In the Neogene crust extruded laterally from the Pamir Plateau to the west by dextral wrenching and E-W extension; this component of deformation is accommodated by E-W shortening in the Afghan-Tajik Depression.

Measurements of Shear Lift Force on a Bubble in Channel Flow in Microgravity

NASA Technical Reports Server (NTRS)

Nahra, Henry K.; Motil, Brian J.; Skor, Mark

2003-01-01

Under microgravity conditions, the shear lift force acting on bubbles, droplets or solid particles in multiphase flows becomes important because under normal gravity, this hydrodynamic force is masked by buoyancy. This force plays an important role in furnishing the detachment process of bubbles in a setting where a bubble suspension is needed in microgravity. In this work, measurements of the shear lift force acting on a bubble in channel flow are performed. The shear lift force is deduced from the bubble kinematics using scaling and then compared with predictions from models in literature that address different asymptotic and numerical solutions. Basic trajectory calculations are then performed and the results are compared with experimental data of position of the bubble in the channel. A direct comparison of the lateral velocity of the bubbles is also made with the lateral velocity prediction from investigators, whose work addressed the shear lift on a sphere in different two-dimensional shear flows including Poiseuille flow.

Design Choices for Thermofluid Flow Components and Systems that are Exported as Functional Mockup Units

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wetter, Michael; Fuchs, Marcus; Nouidui, Thierry

This paper discusses design decisions for exporting Modelica thermofluid flow components as Functional Mockup Units. The purpose is to provide guidelines that will allow building energy simulation programs and HVAC equipment manufacturers to effectively use FMUs for modeling of HVAC components and systems. We provide an analysis for direct input-output dependencies of such components and discuss how these dependencies can lead to algebraic loops that are formed when connecting thermofluid flow components. Based on this analysis, we provide recommendations that increase the computing efficiency of such components and systems that are formed by connecting multiple components. We explain what codemore » optimizations are lost when providing thermofluid flow components as FMUs rather than Modelica code. We present an implementation of a package for FMU export of such components, explain the rationale for selecting the connector variables of the FMUs and finally provide computing benchmarks for different design choices. It turns out that selecting temperature rather than specific enthalpy as input and output signals does not lead to a measurable increase in computing time, but selecting nine small FMUs rather than a large FMU increases computing time by 70%.« less

α-, β-phenomena in the post-symmetry break for the flow past a circular cylinder

NASA Astrophysics Data System (ADS)

Kalita, Jiten C.; Sen, Shuvam

2017-03-01

In the existing literature, the so-called α- and β-phenomena have been reported only for the early stages for the flow past an impulsively started circular cylinder. The current study endeavours to explore the possible existence of these phenomena even in the later stages of the flow. The flow is computed using a recently developed compact finite difference method for the biharmonic form of the two-dimensional Navier-Stokes equations for a wide range of Reynolds numbers (Re). We establish that these secondary phenomena not only appear once the wake becomes asymmetric but also periodically during the post-vortex shedding period for Re = 1000. Further, the recently reported sub-α- and sub-β-phenomena for Re = 5000 at the tertiary level during the early stages of the flow could be identified even during the later stages of the flow as well. The formation of these tertiary structures has been explained through a detailed theoretical characterization of the topological aspects of the boundary layer separation. Both qualitative and quantitative results are provided to substantiate our claim.

The NGC 1023 galaxy group: An anti-hubble flow?

NASA Astrophysics Data System (ADS)

Chernin, A. D.; Dolgachev, V. P.; Domozhilova, L. M.

2010-10-01

We discuss recently published data indicating that the nearby galaxy group NGC 1023 includes an inner, virialized, quasi-stationary component together with an outer component comprising a flow of dwarf galaxies falling toward the center of the system. The inner component is similar to the Local Group of galaxies, but the Local Group is surrounded by a receding set of dwarf galaxies forming the local Hubble flow, rather than a system of approaching dwarfs. This clear difference in the structures of these two systems, which are very similar in other respects, may be associated with the dark energy in which they are immersed. Self-gravity dominates in the inner component of the Local Group, while the anti-gravity created by the cosmic dark-energy background dominates in the surrounding Hubble flow. In contrast, self-gravity likewise dominates throughout the NGC 1023 Group, both in its central component and in the surrounding “anti-Hubble” flow. NGC 1023 as a whole is apparently in an ongoing state of formation and virialization. We expect that there exists a receding flow similar to the local Hubble flow at distances of 1.4-3 Mpc from the center of the group, where anti-gravity should become stronger than the gravity of the system.

Does disseminated nontuberculous mycobacterial disease cause false-positive Determine B-LAM lateral flow assay results? A retrospective review.

PubMed

Nel, Jeremy S; Lippincott, Christopher K; Berhanu, Rebecca; Spencer, David C; Sanne, Ian M; Ive, Prudence

2017-06-02

We retrospectively reviewed the Determine TB-LAM lateral flow assay (LF-LAM) results among HIV-infected patients with disseminated nontuberculous mycobacterial (NTM) disease. LF-LAM was positive in 19/21 patients without evidence of tuberculosis coinfection. Although tuberculosis-NTM coinfection may have been underdiagnosed, our results suggest that disseminated NTM disease may cause false-positive LF-LAM results. © The Author 2017. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.

Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia.

PubMed

Cáceres, Diego H; Zuluaga, Alejandra; Tabares, Ángela M; Chiller, Tom; González, Ángel; Gómez, Beatriz L

2017-12-21

A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS®) and CrAg® LFA was observed. CrAg® LFA showed higher analytical sensitivity for detecting low concentrations of cryptococcal antigen.

Nonlinear Convective Flows in a Laterally Heated Two-Layer System with a Temperature-Dependent Heat Release/Consumption at the Interface

NASA Astrophysics Data System (ADS)

Simanovskii, Ilya; Viviani, Antonio; Dubois, Frank; Queeckers, Patrick

2018-01-01

Nonlinear convective flows developed under the joint action of buoyant and thermocapillary effects in a laterally heated two-layer system filling the closed cavity, have been investigated. The influence of a temperature-dependent interfacial heat release/consumption on nonlinear steady and oscillatory regimes, has been studied. It is shown that sufficiently strong temperature dependence of interfacial heat sinks and heat sources can change the sequence of bifurcations and lead to the development of specific oscillatory regimes in the system.

Evaluation of a Cryptococcal antigen Lateral Flow Assay in serum and cerebrospinal fluid for rapid diagnosis of cryptococcosis in Colombia

PubMed Central

Cáceres, Diego H.; Zuluaga, Alejandra; Tabares, Ángela M.; Chiller, Tom; González, Ángel; Gómez, Beatriz L.

2017-01-01

ABSTRACT A Lateral Flow Assay to detect cryptococcal antigen (CrAg® LFA) in serum and cerebrospinal fluid for the rapid diagnosis of cryptococcosis was evaluated. A retrospective validation was performed. Sensitivity and specificity of the CrAg® LFA was 100%. High concordance (kappa index=1.0) between Cryptococcal Antigen Latex Agglutination System (CALAS®) and CrAg® LFA was observed. CrAg® LFA showed higher analytical sensitivity for detecting low concentrations of cryptococcal antigen. PMID:29267584

Genetic analysis of the gravitropic set-point angle in lateral roots of Arabidopsis

NASA Technical Reports Server (NTRS)

Mullen, J. L.; Hangarter, R. P.; Kiss, J. Z. (Principal Investigator)

2003-01-01

Research on gravity responses in plants has mostly focused on primary roots and shoots, which typically orient to a vertical orientation. However, the distribution of lateral organs and their characteristically non-vertical growth orientation are critical for the determination of plant form. For example, in Arabidopsis, when lateral roots emerge from the primary root, they grow at a nearly horizontal orientation. As they elongate, the roots slowly curve until they eventually reach a vertical orientation. The regulation of this lateral root orientation is an important component affecting overall root system architecture. We found that this change in orientation is not simply due to the onset of gravitropic competence, as non-vertical lateral roots are capable of both positive and negative gravitropism. Thus, the horizontal growth of new lateral roots appears to be determined by what is called the gravitropic set-point angle (GSA). This developmental control of the GSA of lateral roots in Arabidopsis provides a useful system for investigating the components involved in regulating gravitropic responses. Using this system, we have identified several Arabidopsis mutants that have altered lateral root orientations but maintain normal primary root orientation. c2003 COSPAR. Published by Elsevier Ltd. All rights reserved.

Vehicle integration effects on hypersonic waveriders. M.S. Thesis - George Washington Univ.

NASA Technical Reports Server (NTRS)

Cockrell, Charles Edward, Jr.

1994-01-01

The integration of a class of hypersonic high-lift configurations known as waveriders into hypersonic cruise vehicles was evaluated. Waveriders offer advantages in aerodynamic performance and propulsion/airframe integration (PAI) characteristics over conventional hypersonic shapes. A wind-tunnel model was developed which integrates realistic vehicle components with two waverider shapes, referred to as the 'straight-wing' and 'cranked-wing' shapes. Both shapes were conical-flow-derived waveriders at a design Mach number of 4.0. The cranked-wing shape was designed to provide advantages in subsonic performance and directional stability over conventional waveriders. Experimental data and limited computational fluid dynamics (CFD) predictions were obtained over a Mach number range of 2.3 to 4.63 at a Reynolds number of 2.0x10(exp 6) per foot. The CFD predictions and flow visualization data confirmed the shock attachment characteristics of the baseline waverider shapes and illustrated the waverider flow-field properties. Both CFD predictions and experimental data showed that no significant performance degradations occur at off-design Mach numbers for the waverider shapes and the integrated configurations. The experimental data showed that the effects of adding a realistic canopy were minimal. The effects of adding engine components were to increase the drag and thus degrade the aerodynamic performance of the configuration. A significant degradation in aerodynamic performance was observed when 0 degree control surfaces were added to close the blunt base of the waverider to a sharp trailing edge. A comparison of the fully-integrated waverider models to the baseline shapes showed that the performance was significantly degraded when all of the components were added to the waveriders. The fully-integrated configurations studied here do not offer significant performance advantages over conventional hypersonic vehicles, but still offer advantages in air-breathing propulsion integration. Additionally, areas are identified in this study where improvements could be made to enhance the performance. Both fully-integrated configurations are longitudinally unstable over the Mach number range studied for unpowered conditions. The cranked-wing fully-integrated configuration provided significantly better lateral-directional stability characteristics than the straight-wing configuration.

Lateral ring metal elastic wheel absorbs shock loading

NASA Technical Reports Server (NTRS)

Galan, L.

1966-01-01

Lateral ring metal elastic wheel absorbs practically all shock loading when operated over extremely rough terrain and delivers only a negligible shock residue to associated suspension components. The wheel consists of a rigid aluminum assembly to which lateral titanium ring flexible elements with treads are attached.

Ultrasonic Fluid Quality Sensor System

DOEpatents

Gomm, Tyler J.; Kraft, Nancy C.; Phelps, Larry D.; Taylor, Steven C.

2003-10-21

A system for determining the composition of a multiple-component fluid and for determining linear flow comprising at least one sing-around circuit that determines the velocity of a signal in the multiple-component fluid and that is correlatable to a database for the multiple-component fluid. A system for determining flow uses two of the inventive circuits, one of which is set at an angle that is not perpendicular to the direction of flow.

Ultrasonic fluid quality sensor system

DOEpatents

Gomm, Tyler J.; Kraft, Nancy C.; Phelps, Larry D.; Taylor, Steven C.

2002-10-08

A system for determining the composition of a multiple-component fluid and for determining linear flow comprising at least one sing-around circuit that determines the velocity of a signal in the multiple-component fluid and that is correlatable to a database for the multiple-component fluid. A system for determining flow uses two of the inventive circuits, one of which is set at an angle that is not perpendicular to the direction of flow.

Factors affecting femoral rotational angle based on the posterior condylar axis in gap-based navigation-assisted total knee arthroplasty for valgus knee.

PubMed

Lee, Sung-Sahn; Lee, Yong-In; Kim, Dong-Uk; Lee, Dae-Hee; Moon, Young-Wan

2018-01-01

Achieving proper rotational alignment of the femoral component in total knee arthroplasty (TKA) for valgus knee is challenging because of lateral condylar hypoplasia and lateral cartilage erosion. Gap-based navigation-assisted TKA enables surgeons to determine the angle of femoral component rotation (FCR) based on the posterior condylar axis. This study evaluated the possible factors that affect the rotational alignment of the femoral component based on the posterior condylar axis. Between 2008 and 2016, 28 knees were enrolled. The dependent variable for this study was FCR based on the posterior condylar axis, which was obtained from the navigation system archives. Multiple regression analysis was conducted to identify factors that might predict FCR, including body mass index (BMI), Kellgren-Lawrence grade (K-L grade), lateral distal femoral angles obtained from the navigation system and radiographs (NaviLDFA, XrayLDFA), hip-knee-ankle (HKA) axis, lateral gap under varus stress (LGVS), medial gap under valgus stress (MGVS), and side-to-side difference (STSD, MGVS - LGVS). The mean FCR was 6.1° ± 2.0°. Of all the potentially predictive factors evaluated in this study, only NaviLDFA (β = -0.668) and XrayLDFA (β = -0.714) predicted significantly FCR. The LDFAs, as determined using radiographs and the navigation system, were both predictive of the rotational alignment of the femoral component based on the posterior condylar axis in gap-based TKA for valgus knee. A 1° increment with NaviLDFA led to a 0.668° decrement in FCR, and a 1° increment with XrayLDFA led to a 0.714° decrement. This suggests that symmetrical lateral condylar hypoplasia of the posterior and distal side occurs in lateral compartment end-stage osteoarthritis with valgus deformity.

Design and application of a fish-shaped lateral line probe for flow measurement

NASA Astrophysics Data System (ADS)

Tuhtan, J. A.; Fuentes-Pérez, J. F.; Strokina, N.; Toming, G.; Musall, M.; Noack, M.; Kämäräinen, J. K.; Kruusmaa, M.

2016-04-01

We introduce the lateral line probe (LLP) as a measurement device for natural flows. Hydraulic surveys in rivers and hydraulic structures are currently based on time-averaged velocity measurements using propellers or acoustic Doppler devices. The long-term goal is thus to develop a sensor system, which includes spatial gradients of the flow field along a fish-shaped sensor body. Interpreting the biological relevance of a collection of point velocity measurements is complicated by the fact that fish and other aquatic vertebrates experience the flow field through highly dynamic fluid-body interactions. To collect body-centric flow data, a bioinspired fish-shaped probe is equipped with a lateral line pressure sensing array, which can be applied both in the laboratory and in the field. Our objective is to introduce a new type of measurement device for body-centric data and compare its output to estimates of conventional point-based technologies. We first provide the calibration workflow for laboratory investigations. We then provide a review of two velocity estimation workflows, independent of calibration. Such workflows are required as existing field investigations consist of measurements in environments where calibration is not feasible. The mean difference for uncalibrated LLP velocity estimates from 0 to 50 cm/s under in a closed flow tunnel and open channel flume was within 4 cm/s when compared to conventional measurement techniques. Finally, spatial flow maps in a scale vertical slot fishway are compared for the LLP, direct measurements, and 3D numerical models where it was found that the LLP provided a slight overestimation of the current velocity in the jet and underestimated the velocity in the recirculation zone.

Centrifugal study of zone of influence during air-sparging.

PubMed

Hu, Liming; Meegoda, Jay N; Du, Jianting; Gao, Shengyan; Wu, Xiaofeng

2011-09-01

Air sparging (AS) is one of the groundwater remediation techniques for remediating volatile organic compounds (VOCs) in saturated soil. However, in spite of the success of air sparging as a remediation technique for the cleanup of contaminated soils, to date, the fundamental mechanisms or the physics of air flow through porous media is not well understood. In this study, centrifugal modeling tests were performed to investigate air flow rates and the evolution of the zone of influence during the air sparging under various g-levels. The test results show that with the increase in sparging pressure the mass flow rate of the air sparging volume increases. The air mass flow rate increases linearly with the effective sparging pressure ratio, which is the difference between sparging pressure and hydrostatic pressure normalized with respect to the effective overburden pressure at the sparging point. Also the slope of mass flow rate with effective sparging pressure ratio increases with higher g-levels. This variation of the slope of mass flow rate of air sparging volume versus effective sparging pressure ratio, M, is linear with g-level confirming that the air flow through soil for a given effective sparging pressure ratio only depends on the g-level. The test results also show that with increasing sparging pressure, the zone of influence (ZOI), which consists of the width at the tip of the cone or lateral intrusion and the cone angle, will lead to an increase in both lateral intrusion and the cone angle. With a further increase in air injection pressure, the cone angle reaches a constant value while the lateral intrusion becomes the main contributor to the enlargement of the ZOI. However, beyond a certain value of effective sparging pressure ratio, there is no further enlargement of the ZOI.

Interaction Between Downwelling Flow and the Laterally-Varying Thickness of the North American Lithosphere Inferred from Seismic Anisotropy

NASA Astrophysics Data System (ADS)

Behn, M. D.; Conrad, C. P.; Silver, P. G.

2005-12-01

Shear flow in the asthenosphere tends to align olivine crystals in the direction of shear, producing a seismically anisotropic asthenosphere that can be detected using a number of seismic techniques (e.g., shear-wave splitting (SWS) and surface waves). In the ocean basins, where the asthenosphere has a relatively uniform thickness and lithospheric anisotropy appears to be small, observed azimuthal anisotropy is well fit by asthenospheric shear flow in global flow models driven by a combination of plate motions and mantle density heterogeneity. In contrast, beneath the continents both the lithospheric ceiling and asthenospheric thickness may vary considerably across cratonic regions and ocean-continent boundaries. To examine the influence of a continental lithosphere with variable thickness on predictions of continental seismic anisotropy, we impose lateral variations in lithospheric viscosity in global models of mantle flow driven by plate motions and mantle density heterogeneity. For the North American continent, the Farallon slab descends beneath a deep cratonic root, producing downwelling flow in the upper mantle and convergent flow beneath the cratonic lithosphere. We evaluate both the orientation of the predicted azimuthal anisotropy and the depth dependence of radial anisotropy for this downwelling flow and find that the inclusion of a strong continental root provides an improved fit to observed SWS observations beneath the North American craton. Thus, we hypothesize that at least some continental anisotropy is associated with sub-lithospheric viscous shear, although fossil anisotropy in the lithospheric layer may also contribute significantly. Although we do not observe significant variations in the direction of predicted anisotropy with depth, we do find that the inclusion of deep continental roots pushes the depth of the anisotropy layer deeper into the upper mantle. We test several different models of laterally-varying lithosphere and asthenosphere viscosity. These models can be used to separate the contributions of asthenospheric flow and lithospheric fossil fabric in observations of continental anisotropy.

Overview for Design and Construction of Drilled Shafts in Cohesive Soils.

DTIC Science & Technology

1981-08-01

water flowing around supporting columns of bridges. Methods for determining the lateral load -deflection behavior of drilled shafts are based on solutions...PROCEDURES. ..... ............... 22 Axial Load Behavior of Single Shafts .... ......... 22 Lateral Load Behavior of Single Shafts .... ........ 54 Load ...on the shaft (Patey 1977, Claessen and Horvat 1974). Large-diameter shafts can be more easily constructed to resist lateral loads than driven piles or

Research on energy conversion mechanism of rotodynamic pump and design of non-overload centrifugal pump

NASA Astrophysics Data System (ADS)

Zhang, X. L.; Hu, S. B.; Shen, Z. Z.; Wu, S. P.; Li, K.

2016-05-01

In this paper, an attempt has been made for the calculation of an expression for the intrinsic law of input power which has not yet been given by current theory of Rotodynamic pump. By adequate recognition of the characteristics of non-inertial system within the rotating impeller, it is concluded that the input power consists of two power components, the first power component, whose magnitude increases with the increase of the flow rate, corresponds to radial velocity component, and the second power component, whose magnitude decreases with the increase of the flow rate, corresponds to tangential velocity component, therefore, the law of rise, basic levelness and drop of input power curves of centrifugal pump, mixed-flow pump and axial-flow pump can be explained reasonably. Through further analysis, the main ways for realizing non-overload of centrifugal pump are obtained, and its equivalent design factor is found out, the factor correlates with the outlet angle of leading face and back face of the blade, wrap angle, number of blades, outlet width, area ratio, and the ratio of operating flow rate to specified flow rate and so on. These are verified with actual example.

Bumblebee flight performance in environments of different proximity.

PubMed

Linander, Nellie; Baird, Emily; Dacke, Marie

2016-02-01

Flying animals are capable of navigating through environments of different complexity with high precision. To control their flight when negotiating narrow tunnels, bees and birds use the magnitude of apparent image motion (known as optic flow) generated by the walls. In their natural habitat, however, these animals would encounter both cluttered and open environments. Here, we investigate how large changes in the proximity of nearby surfaces affect optic flow-based flight control strategies. We trained bumblebees to fly along a flight and recorded how the distance between the walls--from 60 cm to 240 cm--affected their flight control. Our results reveal that, as tunnel width increases, both lateral position and ground speed become increasingly variable. We also find that optic flow information from the ground has an increasing influence on flight control, suggesting that bumblebees measure optic flow flexibly over a large lateral and ventral field of view, depending on where the highest magnitude of optic flow occurs. A consequence of this strategy is that, when flying in narrow spaces, bumblebees use optic flow information from the nearby obstacles to control flight, while in more open spaces they rely primarily on optic flow cues from the ground.

Pulsatile spiral blood flow through arterial stenosis.

PubMed

Linge, Fabian; Hye, Md Abdul; Paul, Manosh C

2014-11-01

Pulsatile spiral blood flow in a modelled three-dimensional arterial stenosis, with a 75% cross-sectional area reduction, is investigated by using numerical fluid dynamics. Two-equation k-ω model is used for the simulation of the transitional flow with Reynolds numbers 500 and 1000. It is found that the spiral component increases the static pressure in the vessel during the deceleration phase of the flow pulse. In addition, the spiral component reduces the turbulence intensity and wall shear stress found in the post-stenosis region of the vessel in the early stages of the flow pulse. Hence, the findings agree with the results of Stonebridge et al. (2004). In addition, the results of the effects of a spiral component on time-varying flow are presented and discussed along with the relevant pathological issues.

Design flow factors for sewerage systems in small arid communities.

PubMed

Imam, Emad H; Elnakar, Haitham Y

2014-09-01

Reliable estimation of sewage flow rates is essential for the proper design of sewers, pumping stations, and treatment plants. The design of the various components of the sewerage system should be based on the most critical flow rates with a focus on extremely low and peak flow rates that would be sustained for a duration related to the acceptable limits of behavior of the components under consideration. The extreme flow conditions and to what extent they differ from the average values are closely related to the size of the community or network, and the socioeconomic conditions. A single pumping station is usually sufficient to pump flow from small community in either flat or non-undulating topography. Therefore, the hydraulic loading on the wastewater treatment plant (WWTP) results from the pumped flow from the pumping station rather than the trunk sewer flow. The intermittent operation of the pumping units further accentuates the sewage hydrograph in the final trunk sewer. Accordingly, the design flow for the various components of the WWTP should be determined based on their relevant flow factors. In this study, analysis of one representative small community out of five monitored small communities in Egypt and the Kingdom of Saudi Arabia is presented. Pumped sewage flow rates were measured and the sewer incoming flows were hydraulically derived. The hourly and daily sewer and pumped flow records were analyzed to derive the relationship between the flow factors that would be sustained for various durations (instantaneously, 1 h, 2 h, etc.) and their probability of non-exceedance. The resulting peaking factors with a consideration for their sustained flow duration and specified probability would permit the design of the various components of the treatment plant using more accurate critical flows.

Design flow factors for sewerage systems in small arid communities

PubMed Central

Imam, Emad H.; Elnakar, Haitham Y.

2013-01-01

Reliable estimation of sewage flow rates is essential for the proper design of sewers, pumping stations, and treatment plants. The design of the various components of the sewerage system should be based on the most critical flow rates with a focus on extremely low and peak flow rates that would be sustained for a duration related to the acceptable limits of behavior of the components under consideration. The extreme flow conditions and to what extent they differ from the average values are closely related to the size of the community or network, and the socioeconomic conditions. A single pumping station is usually sufficient to pump flow from small community in either flat or non-undulating topography. Therefore, the hydraulic loading on the wastewater treatment plant (WWTP) results from the pumped flow from the pumping station rather than the trunk sewer flow. The intermittent operation of the pumping units further accentuates the sewage hydrograph in the final trunk sewer. Accordingly, the design flow for the various components of the WWTP should be determined based on their relevant flow factors. In this study, analysis of one representative small community out of five monitored small communities in Egypt and the Kingdom of Saudi Arabia is presented. Pumped sewage flow rates were measured and the sewer incoming flows were hydraulically derived. The hourly and daily sewer and pumped flow records were analyzed to derive the relationship between the flow factors that would be sustained for various durations (instantaneously, 1 h, 2 h, etc.) and their probability of non-exceedance. The resulting peaking factors with a consideration for their sustained flow duration and specified probability would permit the design of the various components of the treatment plant using more accurate critical flows. PMID:25685521

Investigation of Separation of the Turbulent Boundary Layer

NASA Technical Reports Server (NTRS)

Schubauer, G B; Klebanoff, P S

1951-01-01

An investigation was conducted on a turbulent boundary layer near a smooth surface with pressure gradients sufficient to cause flow separation. The reynolds number was high, but the speeds were entirely within the incompressible flow range. The investigation consisted of measurements of mean flow, three components of turbulence intensity, turbulent shearing stress, and correlations between two fluctuation components at a point and between the same component of different points. The results are given in the form of tables and graphs. The discussion deals first with separation and then with the more fundamental question of basic concepts of turbulent flow.

Fine sediment trapping in river lateral cavities

NASA Astrophysics Data System (ADS)

Juez, C.; Maechler, G.; Schleiss, A. J.; Franca, M. J.

2016-12-01

River restoration is nowadays a major issue in the field of hydraulics. The natural course and geometry of the rivers have been artificially changed by human activities for different purposes (land gaining, flood protection, agriculture). From a morphologic point of view, channelized rivers often display a straight path and monotonous river banks. This is in contradiction with natural morphology, where a high diversity can be found across the channel path (meanders) and the banks (pools, riffles). One way to restore rivers consist of transforming the artificial banks by adding macro-roughness elements in the lateral river banks (also called cavities and lateral embayments). The creation of irregularities on the banks causes new flow patterns that diversify the river habitat. However, these lateral cavities may be also responsible of the change of the river morphology, since they may trap the fine sediments travelling within the water. This is particularly important in glacier-fed streams such as the upper Rhone River in Switzerland. These are charged with fine sediments resulting from the erosion of the underlying glaciers bottom. The creation of lateral cavities may affect the sediment and morphological equilibrium of the river since these may trap sediments. This work aims to study the influence of the lateral cavities on the transport of fine sediments in the main channel. A set of laboratory experiments were done which covered a wide range of rectangular cavity configurations. Key parameters such as the flow discharge, the aspect ratio of the cavities and the initial sediment concentration were tested. Surface PIV, sediment samples and turbidity temporal records were collected during the experiments. The trapping efficiency of the cavities and the associated flow patterns were analyzed. The resulting conclusions provide a useful information for the future design of river restoration projects.

A Study of the Flow Structure of Tip Vortices on a Hydrofoil

DTIC Science & Technology

1986-11-28

as measured from the flow visualization imager. . . 0 . . . 61 III.10 The vertical location of the tip vortex center as measured from the flow...pressure gra- dients of opposite sign exist on both sides of an airfoil . These gradients induce an inward lateral flow on the suc- tion side and an...And most recently, Cebeci et al. (1986) developed a viscous/inviscid interaction method to calculate the flow around airfoils , emphasizing the

Image-based modelling of lateral magma flow: the Basement Sill, Antarctica

PubMed Central

Mirhadizadeh, Seyed

2017-01-01

The McMurdo Dry Valleys magmatic system, Antarctica, provides a world-class example of pervasive lateral magma flow on a continental scale. The lowermost intrusion (Basement Sill) offers detailed sections through the now frozen particle microstructure of a congested magma slurry. We simulated the flow regime in two and three dimensions using numerical models built on a finite-element mesh derived from field data. The model captures the flow behaviour of the Basement Sill magma over a viscosity range of 1–104 Pa s where the higher end (greater than or equal to 102 Pa s) corresponds to a magmatic slurry with crystal fractions varying between 30 and 70%. A novel feature of the model is the discovery of transient, low viscosity (less than or equal to 50 Pa s) high Reynolds number eddies formed along undulating contacts at the floor and roof of the intrusion. Numerical tracing of particle orbits implies crystals trapped in eddies segregate according to their mass density. Recovered shear strain rates (10−3–10−5 s−1) at viscosities equating to high particle concentrations (around more than 40%) in the Sill interior point to shear-thinning as an explanation for some types of magmatic layering there. Model transport rates for the Sill magmas imply a maximum emplacement time of ca 105 years, consistent with geochemical evidence for long-range lateral flow. It is a theoretically possibility that fast-flowing magma on a continental scale will be susceptible to planetary-scale rotational forces. PMID:28573002

Image-based modelling of lateral magma flow: the Basement Sill, Antarctica.

PubMed

Petford, Nick; Mirhadizadeh, Seyed

2017-05-01

The McMurdo Dry Valleys magmatic system, Antarctica, provides a world-class example of pervasive lateral magma flow on a continental scale. The lowermost intrusion (Basement Sill) offers detailed sections through the now frozen particle microstructure of a congested magma slurry. We simulated the flow regime in two and three dimensions using numerical models built on a finite-element mesh derived from field data. The model captures the flow behaviour of the Basement Sill magma over a viscosity range of 1-10 4  Pa s where the higher end (greater than or equal to 10 2  Pa s) corresponds to a magmatic slurry with crystal fractions varying between 30 and 70%. A novel feature of the model is the discovery of transient, low viscosity (less than or equal to 50 Pa s) high Reynolds number eddies formed along undulating contacts at the floor and roof of the intrusion. Numerical tracing of particle orbits implies crystals trapped in eddies segregate according to their mass density. Recovered shear strain rates (10 -3 -10 -5  s -1 ) at viscosities equating to high particle concentrations (around more than 40%) in the Sill interior point to shear-thinning as an explanation for some types of magmatic layering there. Model transport rates for the Sill magmas imply a maximum emplacement time of ca 10 5 years, consistent with geochemical evidence for long-range lateral flow. It is a theoretically possibility that fast-flowing magma on a continental scale will be susceptible to planetary-scale rotational forces.

Windflow circulation patterns in a coastal dune blowout, south coast of Lake Michigan

USGS Publications Warehouse

Fraser, G.S.; Bennett, S.W.; Olyphant, G.A.; Bauch, N.J.; Ferguson, V.; Gellasch, C.A.; Millard, C.L.; Mueller, B.; O'Malley, P. J.; Way, J.N.; Woodfield, M.C.

1998-01-01

The windflow patterns in a large active blowout in a coastal dune on the southern shore of Lake Michigan were intensively monitored during a two-day period when the predominant winds shifted from onshore (Day 1) to offshore (Day 2). The wind data were used in conjunction with mapped geomorphic features and sedimentologic characteristics to infer the following aspects of blowout evolution: (1) Prevailing winds are transformed considerably once they enter the blowout. Flow separation occurs when offshore winds enter the blowout over the steep back wall. Separated flows may, in turn, induce countercurrent flows within the trough. Flow expansion and deceleration occur when onshore winds enter over gently sloping walls at the front of the blowout. (2) Maximum erosion occurs along the deflationary floor near the entrance to the blowout, and lateral extensional lobes are also expanding the blowout to the east. Sand avalanches down the eastern and western lateral walls toward the deflationary floor where it is moved toward the rear of the blowout and up the ramp at the south end. Sand leaves the blowout as a series of depositional lobes prograding out onto the surface of the host dune along the south and east walls. (3) Vegetation prevents expansion of the blowout in certain directions and impediments to flow, such as slump blocks, alter circulation patterns and sand transport paths. (4) Prevailing onshore winds deflate the floor and promote eastward expansion of lateral erosional lobes, whereas strong flows from the southwest apparently are the main cause of transport up the transportational ramp and over the south wall of the blowout.

4D Floodplain representation in hydrologic flood forecasting using WRFHydro modeling framework

NASA Astrophysics Data System (ADS)

Gangodagamage, C.; Li, Z.; Adams, T.; Ito, T.; Maitaria, K.; Islam, M.; Dhondia, J.

2015-12-01

Floods claim more lives and damage more property than any other category of natural disaster in the Continental U.S. A system that can demarcate local flood boundaries dynamically could help flood prone communities prepare for and even prevent from catastrophic flood events. Lateral distance from the centerline of the river to the right and left floodplains for the water levels coming out of the models at each grid location have not been properly integrated with the national hydrography dataset (NHDPlus). The NHDPlus dataset represents the stream network with feature classes such as rivers, tributaries, canals, lakes, ponds, dams, coastlines, and stream gages. The NHDPlus dataset consists of approximately 2.7 million river reaches defining how surface water drains to the ocean. These river reaches have upstream and downstream nodes and basic parameters such as flow direction, drainage area, reach slope etc. We modified an existing algorithm (Gangodagamage et al., 2007, 2011) to provide lateral distance from the centerline of the river to the right and left floodplains for the flows simulated by models. Previous work produced floodplain boundaries for static river stages (i.e. 3D metric: distance along the main stem, flow depth, lateral distance from river center line). Our new approach introduces the floodplain boundary for variable water levels with the fourth dimension, time. We use modeled flows from WRFHydro and demarcate the right and left lateral boundaries of inundation dynamically. This approach dynamically integrates with high resolution models (e.g., hourly and ~ 1 km spatial resolution) that are developed from recent advancements in high computational power with ground based measurements (e.g., Fluxnet), lateral inundation vectors (direction and spatial extent) derived from multi-temporal remote sensing data (e.g., LiDAR, WorldView 2, Landsat, ASTER, MODIS), and improved representations of the physical processes through multi-parameterizations. Our approach enhances the normalized (streams are at zero elevations) DEM derived upstream flow routing pathways for stream reaches for given water stages as more and more satellite data become available for various flood inundations. Validation of the inundation boundaries is performed using HEC-RAS hydrodynamic model results for selected streams.

Functional expression of P2X4 receptor in capillary endothelial cells of the cochlear spiral ligament and its role in regulating the capillary diameter.

PubMed

Wu, T; Dai, M; Shi, X R; Jiang, Z G; Nuttall, A L

2011-07-01

The cochlear lateral wall generates the endocochlear potential (EP), which creates a driving force for the hair cell transduction current and is essential for normal hearing. Blood flow at the cochlear lateral wall is critically important for maintaining the EP. The vulnerability of the EP to hypoxia suggests that the blood flow in the cochlear lateral wall is dynamically and precisely regulated to meet the changing metabolic needs of the cochlear lateral wall. It has been reported that ATP, an important extracellular signaling molecule, plays an essential role in regulating cochlear blood flow. However, the cellular mechanism underlying ATP-induced regional blood flow changes has not been investigated. In the current study, we demonstrate that 1) the P2X4 receptor is expressed in endothelial cells (ECs) of spiral ligament (SL) capillaries. 2) ATP elicits a characteristic current through P2X4 on ECs in a dose-dependent manner (EC(50) = 0.16 mM). The ATP current has a reversal potential at ∼0 mV; is inhibited by 5-(3-bromophenyl)-1,3-dihydro-2H-benzofuro[3,2-e]-1,4-diazepin-2-one (5-BDBD), LaCl(3), pyridoxal phosphate-6-azo(benzene-2,4-disulfonic acid) tetrasodium salt hydrate (PPADS), and extracellular acidosis; and is less sensitive to α,β-methyleneadenosine 5'-triphosphate (α,β-MeATP) and 2'- and 3'-O-(4-benzoyl-benzoyl) adenosine 5'-triphosphate (BzATP). 3) ATP elicits a transient increase of intracellular Ca(2+) in ECs. 4) In accordance with the above in vitro findings, perilymphatic ATP (1 mM) caused dilation in SL capillaries in vivo by 11.5%. N(ω)-nitro-l-arginine methyl ester hydrochloride (l-NAME), a nonselective inhibitor of nitric oxide synthase, or 5-BDBD, the specific P2X4 inhibitor, significantly blocked the dilation. These findings support our hypothesis that extracellular ATP regulates cochlear lateral blood flow through P2X4 activation in ECs.

Infant growth and later body composition: evidence from the 4-component model.

PubMed

Chomtho, Sirinuch; Wells, Jonathan Ck; Williams, Jane E; Davies, Peter Sw; Lucas, Alan; Fewtrell, Mary S

2008-06-01

Rapid weight gain in infancy is associated with higher body mass index in later life, but its relation with individual body-composition components remains unclear. We aimed to investigate associations between weight gain during different periods in infancy and later fat mass (FM) and fat-free mass (FFM). Body composition was assessed by using the 4-component model, dual-energy X-ray absorptiometry, and anthropometry in 234 healthy UK children and adolescents (105 boys; x +/- SD age: 11.4 +/- 3.8 y). Early growth measurements were prospective in 52 subjects and retrospective in 182. Relative weight gain was calculated as change in SD score (SDS) during different periods. Relative weight gain from 0 to 3 mo and from 3 to 6 mo showed positive relations with childhood FM, waist circumference, and trunk FM that were equivalent to increases in FMI (FM/height(2)) of 0.24 SDS (95% CI: 0.04, 0.44) and 0.50 SDS (0.25, 0.75) per 1-SDS increase in early weight and that were comparable to the effect of current obesity risk factors. Relative weight gain from 0 to 3 mo was also positively associated with later FFMI (FFM/height(2)). Relative weight gain from 6 to 12 mo was not associated with later body composition. Associations were independent of birth weight, sex, puberty, physical activity, socioeconomic class, ethnicity, and parental body mass index. In this Western population, greater relative weight gain during early infancy was positively associated with later FM and central fat distribution and with FFM. Rapid weight gain in infancy may be a risk factor for later adiposity. Early infancy may provide an opportunity for interventions aimed at reducing later obesity risk.

Effects of slope on the dynamics of dilute pyroclastic density currents from May 18th, 1980 Mt. St. Helens eruption

NASA Astrophysics Data System (ADS)

Bendana, S.; Self, S.; Dufek, J.

2012-12-01

The infamous, May 18th, 1980 eruption of Mt St Helens in the state of Washington produced several episodes of pyroclastic density currents (PDCs) including the initial lateral blast, which traveled nearly 30 km, and later PDCs, which filled in the area up to 8 km north of the volcano. The focus of this research is on the later PDCs, which differed from the lateral blast in that they have a higher particle concentration and filled in the topography up to 40 m. While the concentrated portions of the afternoon PDCs followed deep topographic drainages down the steep flanks of the volcano, the dilute overriding cloud partially decoupled to develop fully dilute, turbulent PDCs on the flanks of the volcano (Beeson, D.L. 1988. Proximal Flank Facies of the May 18, 1980 Ignimbrite: Mt. St. Helens, Washington.). The dilute PDCs deposited thin, cross-stratified and stratified pyroclastic deposits, known as the proximal bedded deposits, which differ greatly in depositional characteristics from the thick, massive, poorly-sorted, block-rich deposits associated with the more concentrated portions of the flow. We explore the influence of topography on the formation of these dilute currents and influence of slope on the currents transport and depositional mechanisms. The deposits on steeper slopes (>15°) are fines depleted relative to the proximal bedded deposits on shallower slopes (<15°). Bedform amplitude and wavelength increase with increasing slope, as does the occurrence of regressive dunes. Increasing slope causes an increase in flow velocity and thus an increase in flow turbulence. The fines depleted deposits suggest that fine ash elutriation is more efficient in flows with stronger turbulence. The longer wavelength and amplitudes suggest that bedform morphology is directly related to flow velocity, an important finding since the controls on bedform wavelength and amplitude in density stratified flows remains poorly constrained. The occurrence of regressive dunes, often interpreted as high flow-regime bedforms, on steeper slopes relative to progressive dunes on shallower slopes further attests to the control of velocity and flow regime on bedform morphology. Samples collected from recently exposed deposits and analyzed by grain size measurements, density analyses, and crystal morphoscopy studies further assess modes of origin and transport of dilute PDCs. The collected data will be used to validate numerical models that attempt to quantify the hazards of decoupled, dilute PDCs.

Difference in vascular patterns between transosseous-equivalent and transosseous rotator cuff repair.

PubMed

Urita, Atsushi; Funakoshi, Tadanao; Horie, Tatsunori; Nishida, Mutsumi; Iwasaki, Norimasa

2017-01-01

Vascularity is the important factor of biologic healing of the repaired tissue. The purpose of this study was to clarify sequential vascular patterns of repaired rotator cuff by suture techniques. We randomized 21 shoulders in 20 patients undergoing arthroscopic rotator cuff repair into 2 groups: transosseous-equivalent repair (TOE group, n = 10) and transosseous repair (TO group, n = 11). Blood flow in 4 regions inside the cuff (lateral articular, lateral bursal, medial articular, and medial bursal), in the knotless suture anchor in the TOE group, and in the bone tunnel in the TO group was measured using contrast-enhanced ultrasound at 1 month, 2 months, 3 months, and 6 months postoperatively. The sequential vascular pattern inside the repaired rotator cuff was different between groups. The blood flow in the lateral articular area at 1 month, 2 months, and 3 months (P = .002, .005, and .025) and that in the lateral bursal area at 2 months (P = .031) in the TO group were significantly greater than those in the TOE group postoperatively. Blood flow was significantly greater for the bone tunnels in the TO group than for the knotless suture anchor in the TOE group at 1 month and 2 months postoperatively (P = .041 and .009). This study clarified that the sequential vascular pattern inside the repaired rotator cuff depends on the suture technique used. Bone tunnels through the footprint may contribute to biologic healing by increasing blood flow in the repaired rotator cuff. Copyright © 2017 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Elsevier Inc. All rights reserved.

Numerical Modeling of Methane Leakage from a Faulty Natural Gas Well into Fractured Tight Formations.

PubMed

Moortgat, Joachim; Schwartz, Franklin W; Darrah, Thomas H

2018-03-01

Horizontal drilling and hydraulic fracturing have enabled hydrocarbon recovery from unconventional reservoirs, but led to natural gas contamination of shallow groundwaters. We describe and apply numerical models of gas-phase migration associated with leaking natural gas wells. Three leakage scenarios are simulated: (1) high-pressure natural gas pulse released into a fractured aquifer; (2) continuous slow leakage into a tilted fractured formation; and (3) continuous slow leakage into an unfractured aquifer with fluvial channels, to facilitate a generalized evaluation of natural gas transport from faulty natural gas wells. High-pressure pulses of gas leakage into sparsely fractured media are needed to produce the extensive and rapid lateral spreading of free gas previously observed in field studies. Transport in fractures explains how methane can travel vastly different distances and directions laterally away from a leaking well, which leads to variable levels of methane contamination in nearby groundwater wells. Lower rates of methane leakage (≤1 Mcf/day) produce shorter length scales of gas transport than determined by the high-pressure scenario or field studies, unless aquifers have low vertical permeabilities (≤1 millidarcy) and fractures and bedding planes have sufficient tilt (∼10°) to allow a lateral buoyancy component. Similarly, in fractured rock aquifers or where permeability is controlled by channelized fluvial deposits, lateral flow is not sufficiently developed to explain fast-developing gas contamination (0-3 months) or large length scales (∼1 km) documented in field studies. Thus, current efforts to evaluate the frequency, mechanism, and impacts of natural gas leakage from faulty natural gas wells likely underestimate contributions from small-volume, low-pressure leakage events. © 2018, National Ground Water Association.

Front propagation in a regular vortex lattice: Dependence on the vortex structure.

PubMed

Beauvier, E; Bodea, S; Pocheau, A

2017-11-01

We investigate the dependence on the vortex structure of the propagation of fronts in stirred flows. For this, we consider a regular set of vortices whose structure is changed by varying both their boundary conditions and their aspect ratios. These configurations are investigated experimentally in autocatalytic solutions stirred by electroconvective flows and numerically from kinematic simulations based on the determination of the dominant Fourier mode of the vortex stream function in each of them. For free lateral boundary conditions, i.e., in an extended vortex lattice, it is found that both the flow structure and the front propagation negligibly depend on vortex aspect ratios. For rigid lateral boundary conditions, i.e., in a vortex chain, vortices involve a slight dependence on their aspect ratios which surprisingly yields a noticeable decrease of the enhancement of front velocity by flow advection. These different behaviors reveal a sensitivity of the mean front velocity on the flow subscales. It emphasizes the intrinsic multiscale nature of front propagation in stirred flows and the need to take into account not only the intensity of vortex flows but also their inner structure to determine front propagation at a large scale. Differences between experiments and simulations suggest the occurrence of secondary flows in vortex chains at large velocity and large aspect ratios.

Lateral jet injection into typical combustor flowfields

NASA Technical Reports Server (NTRS)

Lilley, D. G.

1986-01-01

The experimental problem of lateral jet injection into typical flow fields in the absence of combustion was studied. All flow fields being investigated have no expansion of the crossflow (the test section to swirler diameter ratio D/d = 1), after its passage through an optional swirler (with swirl vane angle phi = 0 (swirler removed), 45, and 70 degree). The lateral jet(s) is(are) located one test-section diameter downstream of the test-section inlet (x/D = 1). The lateral jets have round-sectioned nozzles, each of which has an area of 1/100th of the cross sectional area of the crossflow (A sub j/A sub c = 1/100). Jet-to-crossflow velocity ratios of R = v sub j/u sub o = 2, 4, and 6 were investigated. Helium-bubble low visualization, five-hole pitot probe time-mean velocity measurements, and single-wire time-mean velocity and normal and shear stress turbulence data were obtained in the research program.

7. 'FLOW IN CANAL NO. 1, A JOINTLY USED CANAL, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. 'FLOW IN CANAL NO. 1, A JOINTLY USED CANAL, ON MAY 22 WHEN 210 SECOND FEET OF WATER WAS FLOWING. THIS WAS LATER INCREASED TO 240 SECOND FEET FOR A NUMBER OF DAYS TO SATISFY THE DEMANDS OF THE DRY GULCH COMPANY.' 1925 - Irrigation Canals in the Uinta Basin, Duchesne, Duchesne County, UT

A low-speed wind tunnel study of vortex interaction control techniques on a chine-forebody/delta-wing configuration

NASA Technical Reports Server (NTRS)

Rao, Dhanvada M.; Bhat, M. K.

1992-01-01

A low speed wind tunnel evaluation was conducted of passive and active techniques proposed as a means to impede the interaction of forebody chine and delta wing vortices, when such interaction leads to undesirable aerodynamic characteristics particularly in the post stall regime. The passive method was based on physically disconnecting the chine/wing junction; the active technique employed deflection of inboard leading edge flaps. In either case, the intent was to forcibly shed the chine vortices before they encountered the downwash of wing vortices. Flow visualizations, wing pressures, and six component force/moment measurements confirmed the benefits of forced vortex de-coupling at post stall angles of attack and in sideslip, viz., alleviation of post stall zero beta asymmetry, lateral instability and twin tail buffet, with insignificant loss of maximum lift.

Comparison of Lateral Flow Assay, Kidney Inhibition Swab, and Liquid Chromatography-Tandem Mass Spectrometry for the Detection of Penicillin G Residues in Sow Urine.

PubMed

Shelver, Weilin L; Chakrabarty, Shubhashis; Smith, David J

2017-03-01

Sows (n = 126) were administered penicillin G; urine, collected at slaughter, was screened by kidney inhibition swab (KIS; 4 h testing time) and then stored at -80 °C (∼1200 days) until analysis by lateral flow assay (LF, ∼5 min testing time) and tandem quadrupole LC-MS/MS (TQ) analysis. The stability of penicillin in urine during storage was verified using TQ analyses. Quantitative results were well-correlated (R 2 = 0.98) with only a ∼10% decrease in penicillin concentration during the 3-year storage period. KIS retesting of stored samples returned results consistent with the original analyses. Lateral flow assay results were highly correlated with the KIS and TQ results. A KIS positive sample, which was not confirmed by TQ or LF, was assayed by Triple-TOF LC-MS to determine the cause of the apparent false positive. This study suggests LF can be used to quickly and efficiently screen for penicillin G residues before slaughter.

Development and application of a hillslope hydrologic model

USGS Publications Warehouse

Blain, C.A.; Milly, P.C.D.

1991-01-01

A vertically integrated two-dimensional lateral flow model of soil moisture has been developed. Derivation of the governing equation is based on a physical interpretation of hillslope processes. The lateral subsurface-flow model permits variability of precipitation and evapotranspiration, and allows arbitrary specification of soil-moisture retention properties. Variable slope, soil thickness, and saturation are all accommodated. The numerical solution method, a Crank-Nicolson, finite-difference, upstream-weighted scheme, is simple and robust. A small catchment in northeastern Kansas is the subject of an application of the lateral subsurface-flow model. Calibration of the model using observed discharge provides estimates of the active porosity (0.1 cm3/cm3) and of the saturated horizontal hydraulic conductivity (40 cm/hr). The latter figure is at least an order of magnitude greater than the vertical hydraulic conductivity associated with the silty clay loam soil matrix. The large value of hydraulic conductivity derived from the calibration is suggestive of macropore-dominated hillslope drainage. The corresponding value of active porosity agrees well with a published average value of the difference between total porosity and field capacity for a silty clay loam. ?? 1991.

Development of a Rapid Immunochromatographic Lateral Flow Device Capable of Differentiating Phytase Expressed from Recombinant Aspergillus niger phyA2 and Genetically Modified Corn.

PubMed

Zhou, Xiaojin; Hui, Elizabeth; Yu, Xiao-Lin; Lin, Zhen; Pu, Ling-Kui; Tu, Zhiguan; Zhang, Jun; Liu, Qi; Zheng, Jian; Zhang, Juan

2015-05-06

Phytase is a phosphohydrolase considered highly specific for the degradation of phytate to release bound phosphorus for animal consumption and aid in the reduction of environmental nutrient loading. New sources of phytase have been sought that are economically and efficiently productive including the construction of genetically modified (GM) phytase products designed to bypass the costs associated with feed processing. Four monoclonal antibodies (EH10a, FA7, AF9a, and CC1) raised against recombinant Aspergillus niger phyA2 were used to develop a highly specific and sensitive immunochromatographic lateral flow device for rapid detection of transgenic phytase, such as in GM corn. Antibodies sequentially paired and tested along lateral flow strips showed that the EH10a-FA7 antibody pair was able to detect the recombinant yeast-phytase at 5 ng/mL, whereas the AF9a-CC1 antibody pair to GM phytase corn was able to detect at 2 ng/mL. Concurrent to this development, evidence was revealed which suggests that antibody binding sites may be glycosylated.

A numerical study of blood flow using mixture theory

PubMed Central

Wu, Wei-Tao; Aubry, Nadine; Massoudi, Mehrdad; Kim, Jeongho; Antaki, James F.

2014-01-01

In this paper, we consider the two dimensional flow of blood in a rectangular microfluidic channel. We use Mixture Theory to treat this problem as a two-component system: One component is the red blood cells (RBCs) modeled as a generalized Reiner–Rivlin type fluid, which considers the effects of volume fraction (hematocrit) and influence of shear rate upon viscosity. The other component, plasma, is assumed to behave as a linear viscous fluid. A CFD solver based on OpenFOAM® was developed and employed to simulate a specific problem, namely blood flow in a two dimensional micro-channel, is studied. Finally to better understand this two-component flow system and the effects of the different parameters, the equations are made dimensionless and a parametric study is performed. PMID:24791016

A numerical study of blood flow using mixture theory.

PubMed

Wu, Wei-Tao; Aubry, Nadine; Massoudi, Mehrdad; Kim, Jeongho; Antaki, James F

2014-03-01

In this paper, we consider the two dimensional flow of blood in a rectangular microfluidic channel. We use Mixture Theory to treat this problem as a two-component system: One component is the red blood cells (RBCs) modeled as a generalized Reiner-Rivlin type fluid, which considers the effects of volume fraction (hematocrit) and influence of shear rate upon viscosity. The other component, plasma, is assumed to behave as a linear viscous fluid. A CFD solver based on OpenFOAM ® was developed and employed to simulate a specific problem, namely blood flow in a two dimensional micro-channel, is studied. Finally to better understand this two-component flow system and the effects of the different parameters, the equations are made dimensionless and a parametric study is performed.

Integrated acoustic phase separator and multiphase fluid composition monitoring apparatus and method

DOEpatents

Sinha, Dipen N.

2016-01-12

An apparatus and method for down hole gas separation from the multiphase fluid flowing in a wellbore or a pipe, for determining the quantities of the individual components of the liquid and the flow rate of the liquid, and for remixing the component parts of the fluid after which the gas volume may be measured, without affecting the flow stream, are described. Acoustic radiation force is employed to separate gas from the liquid, thereby permitting measurements to be separately made for these two components; the liquid (oil/water) composition is determined from ultrasonic resonances; and the gas volume is determined from capacitance measurements. Since the fluid flows around and through the component parts of the apparatus, there is little pressure difference, and no protection is required from high pressure differentials.

Integrated acoustic phase separator and multiphase fluid composition monitoring apparatus and method

DOEpatents

Sinha, Dipen N

2014-02-04

An apparatus and method for down hole gas separation from the multiphase fluid flowing in a wellbore or a pipe, for determining the quantities of the individual components of the liquid and the flow rate of the liquid, and for remixing the component parts of the fluid after which the gas volume may be measured, without affecting the flow stream, are described. Acoustic radiation force is employed to separate gas from the liquid, thereby permitting measurements to be separately made for these two components; the liquid (oil/water) composition is determined from ultrasonic resonances; and the gas volume is determined from capacitance measurements. Since the fluid flows around and through the component parts of the apparatus, there is little pressure difference, and no protection is required from high pressure differentials.

GSFLOW - Coupled Ground-Water and Surface-Water Flow Model Based on the Integration of the Precipitation-Runoff Modeling System (PRMS) and the Modular Ground-Water Flow Model (MODFLOW-2005)

USGS Publications Warehouse

Markstrom, Steven L.; Niswonger, Richard G.; Regan, R. Steven; Prudic, David E.; Barlow, Paul M.

2008-01-01

The need to assess the effects of variability in climate, biota, geology, and human activities on water availability and flow requires the development of models that couple two or more components of the hydrologic cycle. An integrated hydrologic model called GSFLOW (Ground-water and Surface-water FLOW) was developed to simulate coupled ground-water and surface-water resources. The new model is based on the integration of the U.S. Geological Survey Precipitation-Runoff Modeling System (PRMS) and the U.S. Geological Survey Modular Ground-Water Flow Model (MODFLOW). Additional model components were developed, and existing components were modified, to facilitate integration of the models. Methods were developed to route flow among the PRMS Hydrologic Response Units (HRUs) and between the HRUs and the MODFLOW finite-difference cells. This report describes the organization, concepts, design, and mathematical formulation of all GSFLOW model components. An important aspect of the integrated model design is its ability to conserve water mass and to provide comprehensive water budgets for a location of interest. This report includes descriptions of how water budgets are calculated for the integrated model and for individual model components. GSFLOW provides a robust modeling system for simulating flow through the hydrologic cycle, while allowing for future enhancements to incorporate other simulation techniques.

Fluid mechanics of fusion lasers. Final report, September 11, 1978-June 5, 1980

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shwartz, J; Kulkarny, V A; Ausherman, D A

1980-01-01

Flow loop components required to operate continuous-flow, repetitively-pulsed CO/sub 2/ and KrF laser drivers for ICF were identified and their performance requirements were specified. It was found that the laser flow loops can have a major effect on the laser beam quality and overall efficiency. The pressure wave suppressor was identified as the most critical flow loop component. The performance of vented side-wall suppressors was evaluated both analytically and experimentally and found capable of meeting the performance requirements of the CO/sub 2/ and KrF fusion lasers. All other laser flow loop components are essentially similar to those used in conventional,more » low speed wind tunnels and are therefore well characterized and can be readily incorporated into fusion laser flow systems designs.« less

Lateral blasts at Mount St. Helens and hazard zonation

USGS Publications Warehouse

Crandell, D.R.; Hoblitt, R.P.

1986-01-01

Lateral blasts at andesitic and dacitic volcanoes can produce a variety of direct hazards, including ballistic projectiles which can be thrown to distances of at least 10 km and pyroclastic density flows which can travel at high speed to distances of more than 30 km. Indirect effect that may accompany such explosions include wind-borne ash, pyroclastic flows formed by the remobilization of rock debris thrown onto sloping ground, and lahars. Two lateral blasts occurred at a lava dome on the north flank of Mount St. Helens about 1200 years ago; the more energetic of these threw rock debris northeastward across a sector of about 30?? to a distance of at least 10 km. The ballistic debris fell onto an area estimated to be 50 km2, and wind-transported ash and lapilli derived from the lateral-blast cloud fell on an additional lobate area of at least 200 km2. In contrast, the vastly larger lateral blast of May 18, 1980, created a devastating pyroclastic density flow that covered a sector of as much as 180??, reached a maximum distance of 28 km, and within a few minutes directly affected an area of about 550 km2. The May 18 lateral blast resulted from the sudden, landslide-induced depressurization of a dacite cryptodome and the hydrothermal system that surrounded it within the volcano. We propose that lateral-blast hazard assessments for lava domes include an adjoining hazard zone with a radius of at least 10 km. Although a lateral blast can occur on any side of a dome, the sector directly affected by any one blast probably will be less than 180??. Nevertheless, a circular hazard zone centered on the dome is suggested because of the difficulty of predicting the direction of a lateral blast. For the purpose of long-term land-use planning, a hazard assessment for lateral blasts caused by explosions of magma bodies or pressurized hydrothermal systems within a symmetrical volcano could designate a circular potential hazard area with a radius of 35 km centered on the volcano. For short-term hazard assessments, if seismicity and deformation indicate that magma is moving toward the flank of a volcano, it should be recognized that a landslide could lead to the sudden unloading of a magmatic or hydrothermal system and thereby cause a catastrophic lateral blast. A hazard assessment should assume that a lateral blast could directly affect an area at least 180?? wide to a distance of 35 km from the site of the explosion, irrespective of topography. ?? 1986 Springer-Verlag.

When interflow also percolates: downslope travel distances and hillslope process zones.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jackson, C. Rhett; Bitew, Menberu; Du, Enhao

2014-02-17

In hillslopes with soils characterized by deep regoliths, such as Ultisols,Oxisols, and Alfisols, interflow occurs episodically over impeding layers near and parallel to the soil surface such as low-conductivity B horizons (e.g.Newman et al., 1998; Buttle andMcDonald, 2002; Du et al., In Review), till layers (McGlynn et al., 1999; Bishop et al., 2004), hardpans (McDaniel et al., 2008), C horizons (Detty and McGuire, 2010), and permeable bedrock (Tromp van Meerveld et al., 2007). As perched saturation develops within and above these impeding but permeable horizons, flow moves laterally downslope, but the perched water also continues to percolate through the impedingmore » horizon to the unsaturated soils and saprolite below. Perched water and solutes will eventually traverse the zone of perched saturation above the impeding horizon and then enter and percolate through the impeding horizon. In such flow situations, only lower hillslope segments with sufficient downslope travel distance will deliver water to the riparian zone within the time scale of a storm.farther up the slope, lateral flow within the zone of perched saturation. will act mainly to shift the point of percolation (location where a water packet leaves the downslope flow zone in the upper soil layer and enters the impeding layer) down the hillslope from the point of infiltration. In flatter parts of the hillslope or in areas with little contrast between the conductivities of the upper and impeding soil layers, lateral flow distances will be negligible.« less

Effect of blade outlet angle on radial thrust of single-blade centrifugal pump

NASA Astrophysics Data System (ADS)

Nishi, Y.; Fukutomi, J.; Fujiwara, R.

2012-11-01

Single-blade centrifugal pumps are widely used as sewage pumps. However, a large radial thrust acts on a single blade during pump operation because of the geometrical axial asymmetry of the impeller. This radial thrust causes vibrations of the pump shaft, reducing the service life of bearings and shaft seal devices. Therefore, to ensure pump reliability, it is necessary to quantitatively understand the radial thrust and clarify the behavior and generation mechanism. This study investigated the radial thrust acting on two kinds of single-blade centrifugal impellers having different blade outlet angles by experiments and computational fluid dynamics (CFD) analysis. Furthermore, the radial thrust was modeled by a combination of three components, inertia, momentum, and pressure, by applying an unsteady conservation of momentum to this impeller. As a result, the effects of the blade outlet angle on both the radial thrust and the modeled components were clarified. The total head of the impeller with a blade outlet angle of 16 degrees increases more than the impeller with a blade outlet angle of 8 degrees at a large flow rate. In this case, since the static pressure of the circumference of the impeller increases uniformly, the time-averaged value of the radial thrust of both impellers does not change at every flow rate. On the other hand, since the impeller blade loading becomes large, the fluctuation component of the radial thrust of the impeller with the blade outlet angle of 16 degrees increases. If the blade outlet angle increases, the fluctuation component of the inertia component will increase, but the time-averaged value of the inertia component is located near the origin despite changes in the flow rate. The fluctuation component of the momentum component becomes large at all flow rates. Furthermore, although the time-averaged value of the pressure component is almost constant, the fluctuation component of the pressure component becomes large at a large flow rate. In addition to the increase of the fluctuation component of this pressure component, because the fluctuation component of the inertia and momentum components becomes large (as mentioned above), the radial thrust increases at a large flow rate, as is the case for the impeller with a large blade outlet angle.

Evaluating the potential energy landscape over single molecules at room temperature with lateral force microscopy

NASA Astrophysics Data System (ADS)

Weymouth, Alfred J.; Riegel, Elisabeth; Matencio, Sonia; Giessibl, Franz J.

2018-04-01

One of the challenges of AFM, in contrast to STM, is that the measured signal includes both long-range and short-range components. The most accurate method for removing long-range components is to measure both on and off an adsorbate and to subtract the difference. This on-off method is challenging at room temperature due to thermal drift. By moving to a non-contact scheme in which the lateral component of the force interaction is probed, the measurement is dominated by short-range interactions. We use frequency-modulation lateral force microscopy to measure individual PTCDA molecules adsorbed on Ag/Si(111)-( √{3 }×√{3 } ). By fitting the data to a model potential, we can extract the depth and width of the potential. When the tip is closer to the sample, a repulsive feature can be observed in the data.

A Network Flow Analysis of the Nitrogen Metabolism in Beijing, China.

PubMed

Zhang, Yan; Lu, Hanjing; Fath, Brian D; Zheng, Hongmei; Sun, Xiaoxi; Li, Yanxian

2016-08-16

Rapid urbanization results in high nitrogen flows and subsequent environmental consequences. In this study, we identified the main metabolic components (nitrogen inputs, flows, and outputs) and used ecological network analysis to track the direct and integral (direct + indirect) metabolic flows of nitrogen in Beijing, China, from 1996 to 2012 and to quantify the structure of Beijing's nitrogen metabolic processes. We found that Beijing's input of new reactive nitrogen (Q, which represents nitrogen obtained from the atmosphere or nitrogen-containing materials used in production and consumption to support human activities) increased from 431 Gg in 1996 to 507 Gg in 2012. Flows to the industry, atmosphere, and household, and components of the system were clearly largest, with total integrated inputs plus outputs from these nodes accounting for 31, 29, and 15%, respectively, of the total integral flows for all paths. The flows through the sewage treatment and transportation components showed marked growth, with total integrated inputs plus outputs increasing to 3.7 and 5.2 times their 1996 values, respectively. Our results can help policymakers to locate the key nodes and pathways in an urban nitrogen metabolic system so they can monitor and manage these components of the system.

Finite element modeling of self-potential signals over conduits and fractures in covered karst terrain Peter Bumpus and Sarah Kruse

NASA Astrophysics Data System (ADS)

Bumpus, P. B.; Kruse, S. E.

2013-12-01

A year of continuous monitoring with two grids of 12-15 electrodes each measured self-potential (SP) over two small covered-karst conduits in Tampa, Florida. Positive and negative SP anomalies episodically manifested over conduits, suggesting that conduit flow is dynamic, not static. Various SP flow regimes in the conduits are postulated: flow in the conduit is faster than through surrounding surficial sediment, flow in the conduit is slower than through surrounding sediment, and conduit flow rates match those through the surrounding sediments. It is further postulated that conduits change permeability with inflow and washing out of sediment, especially associated with rain events. Numerical simulations of the postulated flow regimes were run with 2D simulations using the Comsol finite element modeling code. Simulations show that each regime produces different SP patterns. Models simulate the Tampa field setting in which a 1-2 meter-thick high permeability sand layer overlies a low-permeability clay-rich layer. A funnel-shaped conduit breaches both layers. In the models, when the permeability of the conduit sands is equal to surrounding surficial sands, a small (several mV) negative anomaly manifests locally at the conduit. This negative anomaly can be explained as the result of the depression of the SPS surface (the first sediment surface with a change in conductance or streaming potential coefficient) in the conduit. However a permeability difference of as little as 5 to 20 percent between conduit and background can cause an SP anomaly of tens to several hundred millivolts, either positive or negative. When the permeability is higher in the conduit than the surficial sands, lateral flow into the conduit within the sand layer and through the conduit to the underlying aquifer are both high, and the SP signal over the conduit is positive. This may contradict the concept exemplified in other studies that downward flow creates a negative anomaly. In our case the positive voltage is the result of high lateral flow toward a high flux conduit. As a result, the horizontal dimension of the conduit plays a role in whether a positive or negative anomaly is observed locally near the surface, depending on the degree to which the terminus of the inward lateral flow affects voltage over the conduit center. When the conduit has lower permeability than surrounding surficial sediment, models show that the SP anomaly is negative. In this case lateral flow is small to a low-flux conduit and there is little build-up of positive SP to overcome the negative potential associated with the SPS trough.

Does Humeral Component Lateralization in Reverse Shoulder Arthroplasty Affect Rotator Cuff Torque? Evaluation in a Cadaver Model.

PubMed

Chan, Kevin; Langohr, G Daniel G; Mahaffy, Matthew; Johnson, James A; Athwal, George S

2017-10-01

Humeral component lateralization in reverse total shoulder arthroplasty (RTSA) may improve the biomechanical advantage of the rotator cuff, which could improve the torque generated by the rotator cuff and increase internal and external rotation of the shoulder. The purpose of this in vitro biomechanical study was to evaluate the effect of humeral component lateralization (or lateral offset) on the torque of the anterior and posterior rotator cuff. Eight fresh-frozen cadaveric shoulders from eight separate donors (74 ± 8 years; six males, two females) were tested using an in vitro simulator. All shoulders were prescreened for soft tissue deficit and/or deformity before testing. A custom RTSA prosthesis was implanted that allowed five levels of humeral component lateralization (15, 20, 25, 30, 35 mm), which avoided restrictions imposed by commercially available designs. The torques exerted by the anterior and posterior rotator cuff were measured three times and then averaged for varying humeral lateralization, abduction angle (0°, 45°, 90°), and internal and external rotation (-60°, -30°, 0°, 30°, 60°). A three-way repeated measures ANOVA (abduction angle, humeral lateralization, internal rotation and external rotation angles) with a significance level of α = 0.05 was used for statistical analysis. Humeral lateralization only affected posterior rotator cuff torque at 0° abduction, where increasing humeral lateralization from 15 to 35 mm at 60° internal rotation decreased external rotation torque by 1.6 ± 0.4 Nm (95% CI, -0.07 -1.56 Nm; p = 0.06) from 4.0 ± 0.3 Nm to 2.4 ± 0.6 Nm, respectively, but at 60° external rotation increased external rotation torque by 2.2 ± 0.5 Nm (95% CI, -4.2 to -0.2 Nm; p = 0.029) from 6.2 ± 0.5 Nm to 8.3 ± 0.5 Nm, respectively. Anterior cuff torque was affected by humeral lateralization in more arm positions than the posterior cuff, where increasing humeral lateralization from 15 to 35 mm when at 60° internal rotation increased internal rotation torque at 0°, 45°, and 90° abduction by 3.2 ± 0.5 Nm (95% CI, 1.1-5.2 Nm; p = 0.004) from 6.6 ± 0.6 Nm to 9.7 ± 0.6 Nm, 4.0 ± 0.3 Nm (95% CI, 2.8-5.0 Nm; p < 0.001) from 1.7 ± 1.0 Nm to 5.6 ± 0.9 Nm, and 2.2 ± 0.2 Nm (95% CI, 1.4-2.9 Nm; p < 0.001) from 0.6 ± 0.6 Nm to 2.8 ± 0.6 Nm, respectively. In neutral internal and external rotation, increasing humeral lateral offset from 15 to 35 mm increased the internal rotation torque at 45˚ and 90˚ abduction by 1.5 ± 0.3 Nm (95% CI, 0.2-2.7 Nm; p = 0.02) and 1.3 ± 0.2 Nm (95% CI, 0.4-2.3 Nm; p < 0.001), respectively. Humeral component lateralization improves rotator cuff torque. The results of this preliminary in vitro cadaveric study suggest that the lateral offset of the RTSA humeral component plays an important role in the torque generated by the anterior and posterior rotator cuff. However, further studies are needed before clinical application of these results. Increasing humeral offset may have adverse effects, such as the increased risk of implant modularity, increasing tension of the cuff and soft tissues, increased costs often associated with design modifications, and other possible as yet unforeseen negative consequences.

OVERSMART Reporting Tool for Flow Computations Over Large Grid Systems

NASA Technical Reports Server (NTRS)

Kao, David L.; Chan, William M.

2012-01-01

Structured grid solvers such as NASA's OVERFLOW compressible Navier-Stokes flow solver can generate large data files that contain convergence histories for flow equation residuals, turbulence model equation residuals, component forces and moments, and component relative motion dynamics variables. Most of today's large-scale problems can extend to hundreds of grids, and over 100 million grid points. However, due to the lack of efficient tools, only a small fraction of information contained in these files is analyzed. OVERSMART (OVERFLOW Solution Monitoring And Reporting Tool) provides a comprehensive report of solution convergence of flow computations over large, complex grid systems. It produces a one-page executive summary of the behavior of flow equation residuals, turbulence model equation residuals, and component forces and moments. Under the automatic option, a matrix of commonly viewed plots such as residual histograms, composite residuals, sub-iteration bar graphs, and component forces and moments is automatically generated. Specific plots required by the user can also be prescribed via a command file or a graphical user interface. Output is directed to the user s computer screen and/or to an html file for archival purposes. The current implementation has been targeted for the OVERFLOW flow solver, which is used to obtain a flow solution on structured overset grids. The OVERSMART framework allows easy extension to other flow solvers.

Courant number and unsteady flow computation

USGS Publications Warehouse

Lai, Chintu; ,

1993-01-01

The Courant number C, the key to unsteady flow computation, is a ratio of physical wave velocity, ??, to computational signal-transmission velocity, ??, i.e., C = ??/??. In this way, it uniquely relates a physical quantity to a mathematical quantity. Because most unsteady open-channel flows are describable by a set of n characteristic equations along n characteristic paths, each represented by velocity ??i, i = 1,2,....,n, there exist as many as n components for the numerator of C. To develop a numerical model, a numerical integration must be made on each characteristic curve from an earlier point to a later point on the curve. Different numerical methods are available in unsteady flow computation due to the different paths along which the numerical integration is actually performed. For the denominator of C, the ?? defined as ?? = ?? 0 = ??x/??t has been customarily used; thus, the Courant number has the familiar form of C?? = ??/??0. This form will be referred to as ???common Courant number??? in this paper. The commonly used numerical criteria C?? for stability, neutral stability and instability, are imprecise or not universal in the sense that r0 does not always reflect the true maximum computational data-transmission speed of the scheme at hand, i.e., Ctau is no indication for the Courant constraint. In view of this , a new Courant number, called the ???natural Courant number???, Cn, that truly reflects the Courant constraint, has been defined. However, considering the numerous advantages inherent in the traditional C??, a useful and meaningful composite Courant number, denoted by C??* has been formulated from C??. It is hoped that the new aspects of the Courant number discussed herein afford the hydraulician a broader perspective, consistent criteria, and unified guidelines, with which to model various unsteady flows.

Co-evolution and thresholds in arid floodplain wetland ecosystems.

NASA Astrophysics Data System (ADS)

Sandi, Steven; Rodriguez, Jose; Riccardi, Gerardo; Wen, Li; Saintilan, Neil

2017-04-01

Vegetation in arid floodplain wetlands consist of water dependent and flood tolerant species that rely on periodical floods in order to maintain healthy conditions. The floodplain often consist of a complex system of marshes, swamps and lagoons interconnected by a network of streams and poorly defined rills. Over time, feedbacks develop between vegetation and flow paths producing areas of flow obstruction and flow concentration, which combined with depositional and erosional process lead to a continuous change on the position and characteristics of inundation areas. This coevolution of flow paths and vegetation can reach a threshold that triggers major channel transformations and abandonment of wetland areas, in a process that is irreversible. The Macquarie Marshes is a floodplain wetland complex in the semi-arid region of north western NSW, Australia. The site is characterised by a low-gradient topography that leads to channel breakdown processes where the river network becomes practically non-existent and the flow extends over large areas of wetland that later re-join and reform channels exiting the system. Due to a combination of climatic and anthropogenic pressures, the wetland ecosystem in the Macquarie Marshes has deteriorated over the past few decades. This has been linked to decreasing inundation frequencies and extent, with whole areas of flood dependent species such as Water Couch and Common Reed undergoing complete succession to terrestrial species and dryland. In this presentation we provide an overview of an ecogeomorphological model that we have developed in order to simulate the complex dynamics of the marshes. The model combines hydrodynamic, vegetation and channel evolution modules. We focus on the vegetation component of the model and the transitional rules to predict wetland invasion by terrestrial vegetation.

Comparison of Machine Learning methods for incipient motion in gravel bed rivers

NASA Astrophysics Data System (ADS)

Valyrakis, Manousos

2013-04-01

Soil erosion and sediment transport of natural gravel bed streams are important processes which affect both the morphology as well as the ecology of earth's surface. For gravel bed rivers at near incipient flow conditions, particle entrainment dynamics are highly intermittent. This contribution reviews the use of modern Machine Learning (ML) methods implemented for short term prediction of entrainment instances of individual grains exposed in fully developed near boundary turbulent flows. Results obtained by network architectures of variable complexity based on two different ML methods namely the Artificial Neural Network (ANN) and the Adaptive Neuro-Fuzzy Inference System (ANFIS) are compared in terms of different error and performance indices, computational efficiency and complexity as well as predictive accuracy and forecast ability. Different model architectures are trained and tested with experimental time series obtained from mobile particle flume experiments. The experimental setup consists of a Laser Doppler Velocimeter (LDV) and a laser optics system, which acquire data for the instantaneous flow and particle response respectively, synchronously. The first is used to record the flow velocity components directly upstream of the test particle, while the later tracks the particle's displacements. The lengthy experimental data sets (millions of data points) are split into the training and validation subsets used to perform the corresponding learning and testing of the models. It is demonstrated that the ANFIS hybrid model, which is based on neural learning and fuzzy inference principles, better predicts the critical flow conditions above which sediment transport is initiated. In addition, it is illustrated that empirical knowledge can be extracted, validating the theoretical assumption that particle ejections occur due to energetic turbulent flow events. Such a tool may find application in management and regulation of stream flows downstream of dams for stream restoration, implementation of sustainable practices in river and estuarine ecosystems and design of stable river bed and banks.

Influence of slip-surface geometry on earth-flow deformation, Montaguto earth flow, southern Italy

USGS Publications Warehouse

Guerriero, L.; Coe, Jeffrey A.; Revellio, P.; Grelle, G.; Pinto, F.; Guadagno, F.

2016-01-01

We investigated relations between slip-surface geometry and deformational structures and hydrologic features at the Montaguto earth flow in southern Italy between 1954 and 2010. We used 25 boreholes, 15 static cone-penetration tests, and 22 shallow-seismic profiles to define the geometry of basal- and lateral-slip surfaces; and 9 multitemporal maps to quantify the spatial and temporal distribution of normal faults, thrust faults, back-tilted surfaces, strike-slip faults, flank ridges, folds, ponds, and springs. We infer that the slip surface is a repeating series of steeply sloping surfaces (risers) and gently sloping surfaces (treads). Stretching of earth-flow material created normal faults at risers, and shortening of earth-flow material created thrust faults, back-tilted surfaces, and ponds at treads. Individual pairs of risers and treads formed quasi-discrete kinematic zones within the earth flow that operated in unison to transmit pulses of sediment along the length of the flow. The locations of strike-slip faults, flank ridges, and folds were not controlled by basal-slip surface topography but were instead dependent on earth-flow volume and lateral changes in the direction of the earth-flow travel path. The earth-flow travel path was strongly influenced by inactive earth-flow deposits and pre-earth-flow drainages whose positions were determined by tectonic structures. The implications of our results that may be applicable to other earth flows are that structures with strikes normal to the direction of earth-flow motion (e.g., normal faults and thrust faults) can be used as a guide to the geometry of basal-slip surfaces, but that depths to the slip surface (i.e., the thickness of an earth flow) will vary as sediment pulses are transmitted through a flow.

Engineering controllable architecture in matrigel for 3D cell alignment.

PubMed

Jang, Jae Myung; Tran, Si-Hoai-Trung; Na, Sang Cheol; Jeon, Noo Li

2015-02-04

We report a microfluidic approach to impart alignment in ECM components in 3D hydrogels by continuously applying fluid flow across the bulk gel during the gelation process. The microfluidic device where each channel can be independently filled was tilted at 90° to generate continuous flow across the Matrigel as it gelled. The presence of flow helped that more than 70% of ECM components were oriented along the direction of flow, compared with randomly cross-linked Matrigel. Following the oriented ECM components, primary rat cortical neurons and mouse neural stem cells showed oriented outgrowth of neuronal processes within the 3D Matrigel matrix.

A statistical investigation of the single-point pdf of velocity and vorticity based on direct numerical simulations

NASA Technical Reports Server (NTRS)

Mortazavi, M.; Kollmann, W.; Squires, K.

1987-01-01

Vorticity plays a fundamental role in turbulent flows. The dynamics of vorticity in turbulent flows and the effect on single-point closure models were investigated. The approach was to use direct numerical simulations of turbulent flows to investigate the pdf of velocity and vorticity. The preliminary study of homogeneous shear flow has shown that the expectation of the fluctuating pressure gradient, conditioned with a velocity component, is linear in the velocity component, and that the coefficient is independent of velocity and vorticity. In addition, the work shows that the expectation of the pressure gradient, conditioned with a vorticity component, is essentially zero.

An analysis method for multi-component airfoils in separated flow

NASA Technical Reports Server (NTRS)

Rao, B. M.; Duorak, F. A.; Maskew, B.

1980-01-01

The multi-component airfoil program (Langley-MCARF) for attached flow is modified to accept the free vortex sheet separation-flow model program (Analytical Methods, Inc.-CLMAX). The viscous effects are incorporated into the calculation by representing the boundary layer displacement thickness with an appropriate source distribution. The separation flow model incorporated into MCARF was applied to single component airfoils. Calculated pressure distributions for angles of attack up to the stall are in close agreement with experimental measurements. Even at higher angles of attack beyond the stall, correct trends of separation, decrease in lift coefficients, and increase in pitching moment coefficients are predicted.

Gas production and migration in landfills and geological materials.

PubMed

Nastev, M; Therrien, R; Lefebvre, R; Gélinas, P

2001-11-01

Landfill gas, originating from the anaerobic biodegradation of the organic content of waste, consists mainly of methane and carbon dioxide, with traces of volatile organic compounds. Pressure, concentration and temperature gradients that develop within the landfill result in gas emissions to the atmosphere and in lateral migration through the surrounding soils. Environmental and safety issues associated with the landfill gas require control of off-site gas migration. The numerical model TOUGH2-LGM (Transport of Unsaturated Groundwater and Heat-Landfill Gas Migration) has been developed to simulate landfill gas production and migration processes within and beyond landfill boundaries. The model is derived from the general non-isothermal multiphase flow simulator TOUGH2, to which a new equation of state module is added. It simulates the migration of five components in partially saturated media: four fluid components (water, atmospheric air, methane and carbon dioxide) and one energy component (heat). The four fluid components are present in both the gas and liquid phases. The model incorporates gas-liquid partitioning of all fluid components by means of dissolution and volatilization. In addition to advection in the gas and liquid phase, multi-component diffusion is simulated in the gas phase. The landfill gas production rate is proportional to the organic substrate and is modeled as an exponentially decreasing function of time. The model is applied to the Montreal's CESM landfill site, which is located in a former limestone rock quarry. Existing data were used to characterize hydraulic properties of the waste and the limestone. Gas recovery data at the site were used to define the gas production model. Simulations in one and two dimensions are presented to investigate gas production and migration in the landfill, and in the surrounding limestone. The effects of a gas recovery well and landfill cover on gas migration are also discussed.

Tibial component coverage based on bone mineral density of the cut tibial surface during unicompartmental knee arthroplasty: clinical relevance of the prevention of tibial component subsidence.

PubMed

Lee, Yong Seuk; Yun, Ji Young; Lee, Beom Koo

2014-01-01

An optimally implanted tibial component during unicompartmental knee arthroplasty would be flush with all edges of the cut tibial surface. However, this is often not possible, partly because the tibial component may not be an ideal shape or because the ideal component size may not be available. In such situations, surgeons need to decide between component overhang and underhang and as to which sites must be covered and which sites could be undercovered. The objectives of this study were to evaluate the bone mineral density of the cut surface of the proximal tibia around the cortical rim and to compare the bone mineral density according to the inclusion of the cortex and the site-specific matched evaluation. One hundred and fifty consecutive patients (100 men and 50 women) were enrolled in this study. A quantitative computed tomography was used to determine the bone density of the cut tibial surface. Medial and lateral compartments were divided into anterior, middle, and posterior regions, and these three regions were further subdivided into two regions according to containment of cortex. The site-specific matched comparison (medial vs. lateral) of bone mineral density was performed. In medial sides, the mid-region, including the cortex, showed the highest bone mineral density in male and female patients. The posterior region showed the lowest bone mineral density in male patients, and the anterior and posterior regions showed the lowest bone mineral density in female patients. Regions including cortex showed higher bone mineral density than pure cancellous regions in medial sides. In lateral sides, posterior regions including cortex showed highest bone mineral density with statistical significance in both male and female patients. The anterior region showed the lowest bone mineral density in both male and female patients. The mid-region of the medial side and the posterior region of the lateral side are relatively safe without cortical coverage when the component is not flush with all edges of the tibia. Cortical coverage is strongly recommended for the prevention of subsidence of the tibial component in the posterior region of the medial side, and in the anterior region of the lateral side.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adams, David P.; Fishgrab, Kira L.; Greth, Karl Douglas

The present invention relates to a lateral via to provide an electrical connection to a buried conductor. In one instance, the buried conductor is a through via that extends along a first dimension, and the lateral via extends along a second dimension that is generally orthogonal to the first dimension. In another instance, the second dimension is oblique to the first dimension. Components having such lateral vias, as well as methods for creating such lateral vias are described herein.

Rapid Identification of OXA-48 and OXA-163 Subfamilies in Carbapenem-Resistant Gram-Negative Bacilli with a Novel Immunochromatographic Lateral Flow Assay.

PubMed

Pasteran, Fernando; Denorme, Laurence; Ote, Isabelle; Gomez, Sonia; De Belder, Denise; Glupczynski, Youri; Bogaerts, Pierre; Ghiglione, Barbara; Power, Pablo; Mertens, Pascal; Corso, Alejandra

2016-11-01

We assessed a novel immunochromatographic lateral flow assay for direct identification of OXA-48-like carbapenemases and accurate differentiation of allele variants with distinct substrate profiles (OXA-48 or OXA-163 subfamilies). The assay allowed rapid (less than 4 min) and reliable direct confirmation of OXA-163- and/or OXA-48-like enzymes (with 100% sensitivity and 100% specificity) from cultured colonies that were recovered from both solid medium and spiked blood culture bottles. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Associations between birth weight and later body composition: evidence from the 4-component model.

PubMed

Chomtho, Sirinuch; Wells, Jonathan C K; Williams, Jane E; Lucas, Alan; Fewtrell, Mary S

2008-10-01

Higher birth weight is associated with higher body mass index, traditionally interpreted as greater fatness or obesity, in later life. However, its relation with individual body-composition components and fat distribution remains unclear. We investigated associations between birth weight and later fat mass (FM), fat-free mass (FFM), and fat distribution. Body composition was assessed by the criterion 4-component model in 391 healthy children [mean (+/-SD) age, 11.7 +/- 4.2 y; 188 boys]. FM and FFM were adjusted for height (FMI = FM/height(2); FFMI = FFM/height(2)) and were expressed as SD scores (SDS). Findings were compared between the 4-component and simpler methods. Birth weight was positively associated with height in both sexes and was significantly positively associated with FFMI in boys, equivalent to a 0.18 SDS (95% CI: 0.04, 0.32) increase in FFMI per 1 SDS increase in birth weight. These associations were independent of puberty, physical activity, social class, ethnicity, and parental body mass index. Birth weight was not significantly related to percentage fat, FMI, or trunk FMI in either sex. Equivalent analyses using simpler methods showed a trend for a positive relation between birth weight and FMI in boys that became nonsignificant after adjusting for confounders. FFMI in later life in males is influenced by birth weight, a proxy for prenatal growth, but evidence for fetal programming of later FM or central adiposity is weak. Different body-composition techniques and data interpretation can influence results and should be considered when comparing studies.

Significance of Bioturbated Layer (BTL) and Deep Groundwater Storage on Runoff in Steep Saprolitic Tropical Lowlands Catchment

NASA Astrophysics Data System (ADS)

Cheng, Y.; Ogden, F. L.; Zhu, J.

2016-12-01

Bioturbated soil layers (BTLs) play a significant role in hydrological response and provisioning of ecosystem services in steep, saprolitic, tropical lowlands catchments. In this study, a new physically-based model formulation was developed for testing of runoff generation hypotheses. A main feature in the model formulation is explicit simulation of hydrological processes in the BTL including macropores, which our field observations show are ubiquitous, and deep groundwater stores that provide streamflow during the dry season The numerical model developed includes two main flow paths in the BTL, including one-dimensional (1D) vertical infiltration and two-dimensional (2D) lateral flows in both macropores and the soil matrix. Hydrological processes incorporated along with the BTL processes include intercepted rainfall, evapotranspiration, 2D surface flow and 1D deep groundwater discharge. This model was first tested in a 6.5 ha secondary succession catchment, that is under study by the Smithsonian Tropical Research Institute, Agua Salud project in Panama, which is dominated by steep slopes. With the incorporation of lateral macropore flow mechanism in the BTL, the model performs better than only including soil matrix flow in the BTL especially in simulating baseflow dynamics, which illustrates the importance of preferential flow from the BTL to stream discharge dynamics. The increase in the BTL thickness promotes more flow through the BTL and increases storage in both the BTL and the deep groundwater reservoir, but decreases the total streamflow and overland flow. Lateral macropore diameter distribution influences flows more than the macropore number or distribution type. The model has thus far passed falsification tests during the early wet season. Complexity in subsurface storage and base flow generation offer a new challenge for this model. The overall objective is to develop a model formulation that is useful in practical applications related to land-use management, provisioning of ecosystem services, and water security in similar tropical settings with distinct dry and wet seasons or in the humid tropics during periods of drought.

Longitudinal variation in lateral trapping of fine sediment in tidal estuaries: observations and a 3D exploratory model

NASA Astrophysics Data System (ADS)

Chen, Wei; de Swart, Huib E.

2018-03-01

This study investigates the longitudinal variation of lateral entrapment of suspended sediment, as is observed in some tidal estuaries. In particular, field data from the Yangtze Estuary are analysed, which reveal that in one cross-section, two maxima of suspended sediment concentration (SSC) occur close to the south and north sides, while in a cross-section 2 km down-estuary, only one SSC maximum on the south side is present. This pattern is found during both spring tide and neap tide, which are characterised by different intensities of turbulence. To understand longitudinal variation in lateral trapping of sediment, results of a new three-dimensional exploratory model are analysed. The hydrodynamic part contains residual flow due to fresh water input, density gradients and Coriolis force and due to channel curvature-induced leakage. Moreover, the model includes a spatially varying eddy viscosity that accounts for variation of intensity of turbulence over the spring-neap cycle. By imposing morphodynamic equilibrium, the two-dimensional distribution of sediment in the domain is obtained analytically by a novel procedure. Results reveal that the occurrence of the SSC maxima near the south side of both cross-sections is due to sediment entrapment by lateral density gradients, while the second SSC maximum near the north side of the first cross-section is by sediment transport due to curvature-induced leakage. Coriolis deflection of longitudinal flow also contributes the trapping of sediment near the north side. This mechanism is important in the upper estuary, where the flow due to lateral density gradients is weak.

Giant oscillations of the current in a dirty 2D electron system flowing perpendicular to a lateral barrier under magnetic field

NASA Astrophysics Data System (ADS)

Kadigrobov, A. M.

2017-08-01

The charge transport in a dirty 2-dimensional electron system biased in the presence of a lateral potential barrier under magnetic field is theoretically studied. The quantum tunnelling across the barrier provides the quantum interference of the edge states localized on its both sides that results in giant oscillations of the charge current flowing perpendicular to the lateral junction. Our theoretical analysis is in a good agreement with the experimental observations presented in Kang et al. [Lett. Nat. 403, 59 (2000)]. In particular, positions of the conductance maxima coincide with the Landau levels while the conductance itself is essentially suppressed even at the energies at which the resonant tunnelling occurs and hence these puzzling observations can be resolved without taking into account the electron-electron interaction.

Coevolution of bed surface patchiness and channel morphology: 1. Mechanisms of forced patch formation

USGS Publications Warehouse

Nelson, Peter A.; McDonald, Richard R.; Nelson, Jonathan M.; Dietrich, William E.

2015-01-01

Riverbeds frequently display a spatial structure where the sediment mixture composing the channel bed has been sorted into discrete patches of similar grain size. Even though patches are a fundamental feature in gravel bed rivers, we have little understanding of how patches form, evolve, and interact. Here we present a two-dimensional morphodynamic model that is used to examine in greater detail the mechanisms responsible for the development of forced bed surface patches and the coevolution of bed morphology and bed surface patchiness. The model computes the depth-averaged channel hydrodynamics, mixed-grain-size sediment transport, and bed evolution by coupling the river morphodynamic model Flow and Sediment Transport with Morphological Evolution of Channels (FaSTMECH) with a transport relation for gravel mixtures and the mixed-grain-size Exner equation using the active layer assumption. To test the model, we use it to simulate a flume experiment in which the bed developed a sequence of alternate bars and temporally and spatially persistent forced patches with a general pattern of coarse bar tops and fine pools. Cross-stream sediment flux causes sediment to be exported off of bars and imported into pools at a rate that balances downstream gradients in the streamwise sediment transport rate, allowing quasi-steady bar-pool topography to persist. The relative importance of lateral gravitational forces on the cross-stream component of sediment transport is a primary control on the amplitude of the bars. Because boundary shear stress declines as flow shoals over the bars, the lateral sediment transport is increasingly size selective and leads to the development of coarse bar tops and fine pools.

Flow Components in a NaK Test Loop Designed to Simulate Conditions in a Nuclear Surface Power Reactor

NASA Astrophysics Data System (ADS)

Polzin, Kurt A.; Godfroy, Thomas J.

2008-01-01

A test loop using NaK as the working fluid is presently in use to study material compatibility effects on various components that comprise a possible nuclear reactor design for use on the lunar surface. A DC electromagnetic (EM) pump has been designed and implemented as a means of actively controlling the NaK flow rate through the system and an EM flow sensor is employed to monitor the developed flow rate. These components allow for the matching of the flow rate conditions in test loops with those that would be found in a full-scale surface-power reactor. The design and operating characteristics of the EM pump and flow sensor are presented. In the EM pump, current is applied to a set of electrodes to produce a Lorentz body force in the fluid. A measurement of the induced voltage (back-EMF) in the flow sensor provides the means of monitoring flow rate. Both components are compact, employing high magnetic field strength neodymium magnets thermally coupled to a water-cooled housing. A vacuum gap limits the heat transferred from the high temperature NaK tube to the magnets and a magnetically-permeable material completes the magnetic circuit. The pump is designed to produce a pressure rise of 34.5 kPa, and the flow sensor's predicted output is roughly 20 mV at the loop's nominal flow rate of 0.114 m3/hr.

Flow Components in a NaK Test Loop Designed to Simulate Conditions in a Nuclear Surface Power Reactor

NASA Technical Reports Server (NTRS)

Polzin, Kurt A.; Godfroy, Thomas J.

2008-01-01

A test loop using NaK as the working fluid is presently in use to study material compatibility effects on various components that comprise a possible nuclear reactor design for use on the lunar surface. A DC electromagnetic (EM) pump has been designed and implemented as a means of actively controlling the NaK flow rate through the system and an EM flow sensor is employed to monitor the developed flow rate. These components allow for the matching of the flow rate conditions in test loops with those that would be found in a full-scale surface-power reactor. The design and operating characteristics of the EM pump and flow sensor are presented. In the EM pump, current is applied to a set of electrodes to produce a Lorentz body force in the fluid. A measurement of the induced voltage (back-EMF) in the flow sensor provides the means of monitoring flow rate. Both components are compact, employing high magnetic field strength neodymium magnets thermally coupled to a water-cooled housing. A vacuum gap limits the heat transferred from the high temperature NaK tube to the magnets and a magnetically-permeable material completes the magnetic circuit. The pump is designed to produce a pressure rise of 5 psi, and the flow sensor's predicted output is roughly 20 mV at the loop's nominal flow rate of 0.5 GPM.

Identification of early B cell precursors (stage 1 and 2 hematogones) in the peripheral blood.

PubMed

Kurzer, Jason H; Weinberg, Olga K

2018-05-25

Differentiating malignant B-lymphoblasts from early benign B cell precursors (hematogones) is a vital component of the diagnosis of B-lymphoblastic leukaemia. It has been previously reported that only late-stage B cell precursors circulate in the peripheral blood. Consequently, flow cytometric detection of cells with immunophenotypic findings similar to earlier stage precursors in the peripheral blood justifiably raises concern for involvement by B-lymphoblastic leukaemia. We report here, however, that benign early B cell precursors can indeed be detected in the peripheral blood, thus complicating the interpretation of flow cytometric findings derived from these sample types. A retrospective search of our collective databases identified 13 cases containing circulating early stage B cell precursors. The patients ranged in age from 15 days to 85 years old. All positive cases demonstrated that the earlier B cell precursors were associated with later stage precursors, a finding that could help differentiate these cells from B-lymphoblastic leukaemia. © Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.

Investigating Water Movement Within and Near Wells Using Active Point Heating and Fiber Optic Distributed Temperature Sensing

PubMed Central

Selker, Frank; Selker, John S.

2018-01-01

There are few methods to provide high-resolution in-situ characterization of flow in aquifers and reservoirs. We present a method that has the potential to quantify lateral and vertical (magnitude and direction) components of flow with spatial resolution of about one meter and temporal resolution of about one day. A fiber optic distributed temperature sensor is used with a novel heating system. Temperatures before heating may be used to evaluate background geothermal gradient and vertical profile of thermal diffusivity. The innovation presented is the use of variable energy application along the well, in this case concentrated heating at equally-spaced (2 m) localized areas (0.5 m). Relative to uniform warming this offers greater opportunity to estimate water movement, reduces required heating power, and increases practical length that can be heated. Numerical simulations are presented which illustrate expected behaviors. We estimate relative advection rates near the well using the times at which various locations diverge from a heating trajectory expected for pure conduction in the absence of advection. The concept is demonstrated in a grouted 600 m borehole with 300 heated patches, though evidence of vertical water movement was not seen. PMID:29596339

Implementing a conceptual model of physical and chemical soil profile evolution

NASA Astrophysics Data System (ADS)

Kirkby, Mike

2017-04-01

When soil profile composition is generalised in terms of the proportion, p, of bedrock remaining (= 1 - depletion ratio), then other soil processes can also be expressed in terms of p, and 'soil depth' described by the integral of (1-p) down to bedrock. Soil profile evolution is expressed as the advance of a sigmoidal weathering front into the critical zone under the action of upward ionic diffusion of weathering products; downward advection of solutes in percolating waters, with loss of (cleanish) water as evapotranspiration and (solute-laden) water as a lateral sub-surface flow increment; and mechanical denudation increment at the surface. Each component responds to the degree of weathering. Percolation is limited by precipitation, evapotranspiration demand and the degree of weathering at each level in the profile which diverts subsurface flow. Mechanical removal rates are considered to broadly increase as weathering proceeds, as grain size and dilation angle decreases. The implication of these assumptions can be examined for steady state profiles, for which observed relationships between mechanical and chemical denudation rates; and between chemical denudation and critical zone depth are reproduced. For non-steady state evolution, these relationships break down, but provide a basis for linking critical zone with hillslope/ landform evolution.

Investigating Water Movement Within and Near Wells Using Active Point Heating and Fiber Optic Distributed Temperature Sensing.

PubMed

Selker, Frank; Selker, John S

2018-03-29

There are few methods to provide high-resolution in-situ characterization of flow in aquifers and reservoirs. We present a method that has the potential to quantify lateral and vertical (magnitude and direction) components of flow with spatial resolution of about one meter and temporal resolution of about one day. A fiber optic distributed temperature sensor is used with a novel heating system. Temperatures before heating may be used to evaluate background geothermal gradient and vertical profile of thermal diffusivity. The innovation presented is the use of variable energy application along the well, in this case concentrated heating at equally-spaced (2 m) localized areas (0.5 m). Relative to uniform warming this offers greater opportunity to estimate water movement, reduces required heating power, and increases practical length that can be heated. Numerical simulations are presented which illustrate expected behaviors. We estimate relative advection rates near the well using the times at which various locations diverge from a heating trajectory expected for pure conduction in the absence of advection. The concept is demonstrated in a grouted 600 m borehole with 300 heated patches, though evidence of vertical water movement was not seen.

Following a river wherever it goes: beneath the surface of mountain streams.

Treesearch

Jonathan Thompson; Sally Duncan

2004-01-01

The flow of a mountain stream is difficult to follow, especially when it weaves in and out of the channel, flowing through streambanks and seeping through the streambed. Flowing belowground, the stream water mixes with ground water in the riparian aquifer before reemerging in the channel, sometime later and somewhere further downstream. Underground, the water undergoes...

Turbulent Friction in the Boundary Layer of a Flat Plate in a Two-Dimensional Compressible Flow at High Speeds

NASA Technical Reports Server (NTRS)

Frankl, F.; Voishel, V.

1943-01-01

In the present report an investigation is made on a flat plate in a two-dimensional compressible flow of the effect of compressibility and heating on the turbulent frictional drag coefficient in the boundary layer of an airfoil or wing radiator. The analysis is based on the Prandtl-Karman theory of the turbulent boundary later and the Stodola-Crocco, theorem on the linear relation between the total energy of the flow and its velocity. Formulas are obtained for the velocity distribution and the frictional drag law in a turbulent boundary later with the compressibility effect and heat transfer taken into account. It is found that with increase of compressibility and temperature at full retardation of the flow (the temperature when the velocity of the flow at a given point is reduced to zero in case of an adiabatic process in the gas) at a constant R (sub x), the frictional drag coefficient C (sub f) decreased, both of these factors acting in the same sense.

Identifying hydrological regime and eco-flow threshold of small and medium flood of the Xiaoqing River in Jinan city

NASA Astrophysics Data System (ADS)

Liu, Yang; Cao, Sheng-Le

2017-06-01

It was known that hydrological regime was the main influencing factor of river ecosystem, but the regime of different flow rates of urban rivers was poorly understood. We collected daily inflows at the Huangtai station of the Xiaoqing River from 1960 to 2014 and divided the data into three periods. Then we calculated hydrological parameters by the method of EFCs (Environmental Flow Components) and analyzed the tendency and change rates of each component respectively in the three periods. Combined with the ecological significance of environmental flow components, we identified the small and medium flood had the greatest impact on the river regime and ecosystem. And then we used the hydraulic parameters in the good ecosystem period as control conditions, to calculate the ecological threshold of the flow component under the current situation. This study could provide technical support for restoring and improving hydrological regime and ecological environment of the Xiaoqing River in Jinan city.

The Madden-Julian Oscillation and the Indo-Pacific Warm Pool

NASA Astrophysics Data System (ADS)

Raymond, David J.; Fuchs, Željka

2018-04-01

A minimal model of the interaction of the Madden-Julian oscillation (MJO) with the Indo-Pacific warm pool is presented. This model is based on the linear superposition of the flow associated with a highly simplified treatment of the MJO plus the flow induced by the warm pool itself. Both of these components parameterize rainfall as proportional to the column water vapor, which in turn is governed by a linearized moisture equation in which WISHE (wind induced surface heat exchange) plays a governing role. The MJO component has maximum growth rate for planetary wavenumber 1 and is equatorially trapped with purely zonal winds. The warm pool component exhibits a complex flow pattern, differing significantly from the classical Gill model as a result of the mean easterly flow. The combination of the two produce a flow that reproduces many aspects of the observed global flow associated with the MJO.

Migrating Toward Fully 4-D Geodynamical Models of Asthenospheric Circulation and Melt Production at Mid-Ocean Ridges

NASA Astrophysics Data System (ADS)

van Dam, L.; Kincaid, C. R.; Pockalny, R. A.; Sylvia, R. T.; Hall, P. S.

2017-12-01

Lateral migration of mid-ocean ridge spreading centers is a well-documented phenomenon leading to asymmetric melt production and the surficial expressions thereof. This form of plate motion has been difficult to incorporate into both numerical and analogue geodynamical models, and consequently, current estimates of time-dependent flow, material transport, and melting in the mantle beneath ridges are lacking. To address this, we have designed and built an innovative research apparatus that allows for precise and repeatable simulations of mid-ocean ridge spreading and migration. Three pairs of counter-rotating belts with adjustable lateral orientations are scaled to simulate spreading at, and flow beneath, three 600km wide ridge segments with up to 300km transform offsets. This apparatus is attached to a drive system that allows us to test a full range of axis-parallel to axis-normal migration directions, and is suspended above a reservoir of viscous glucose syrup, a scaled analogue for the upper mantle, and neutrally buoyant tracers. We image plate-driven flow in the syrup with high-resolution digital cameras and use particle image velocimetry methods to obtain information about transport pathlines and flow-induced anisotropy. Suites of experiments are run with and without ridge migration to determine the overall significance of migration on spatial and temporal characteristics of shallow mantle flow. Our experiments cover an expansive parameter space by including various spreading rates, migration speeds and directions, degrees of spreading asymmetry, transform-offset lengths, and upper mantle viscosity conditions. Preliminary results highlight the importance of modeling migratory plate forces. Mantle material exhibits a significant degree of lateral transport, particularly between ridge segments and towards the melt triangle. Magma supply to the melting region is highly complex; parcels of material do not necessarily move along fixed streamlines, rather, they can be perturbed upwards and left behind as spreading centers continue to move laterally. These results emphasize that observations of seismic anisotropy should be interpreted in light of intricate flow pathlines, and that melt transport models should consider different paths for melt relative to the solid matrix.

Flow rate calibration to determine cell-derived microparticles and homogeneity of blood components.

PubMed

Noulsri, Egarit; Lerdwana, Surada; Kittisares, Kulvara; Palasuwan, Attakorn; Palasuwan, Duangdao

2017-08-01

Cell-derived microparticles (MPs) are currently of great interest to screening transfusion donors and blood components. However, the current approach to counting MPs is not affordable for routine laboratory use due to its high cost. The current study aimed to investigate the potential use of flow-rate calibration for counting MPs in whole blood, packed red blood cells (PRBCs), and platelet concentrates (PCs). The accuracy of flow-rate calibration was investigated by comparing the platelet counts of an automated counter and a flow-rate calibrator. The concentration of MPs and their origins in whole blood (n=100), PRBCs (n=100), and PCs (n=92) were determined using a FACSCalibur. The MPs' fold-changes were calculated to assess the homogeneity of the blood components. Comparing the platelet counts conducted by automated counting and flow-rate calibration showed an r 2 of 0.6 (y=0.69x+97,620). The CVs of the within-run and between-run variations of flow-rate calibration were 8.2% and 12.1%, respectively. The Bland-Altman plot showed a mean bias of -31,142platelets/μl. MP enumeration revealed both the difference in MP levels and their origins in whole blood, PRBCs, and PCs. Screening the blood components demonstrated high heterogeneity of the MP levels in PCs when compared to whole blood and PRBCs. The results of the present study suggest the accuracy and precision of flow-rate calibration for enumerating MPs. This flow-rate approach is affordable for assessing the homogeneity of MPs in blood components in routine laboratory practice. Copyright © 2017 Elsevier Ltd. All rights reserved.

Earthflow yield strength constrained by lateral levee morphology

NASA Astrophysics Data System (ADS)

Nereson, A. L.; Finnegan, N. J.

2015-12-01

Slow-moving landslides, or earthflows, are characterized by persistent, flow-like motion that is commonly modeled using various viscous and viscoplastic rheologies. One of the manifestations of viscoplastic flow down a slope is the emergence of stationary bodies of fluid at the margins of the flow (i.e. lateral levees). These levees are common signatures of earthflow morphology and, while they are frequently used to outline boundaries for mapping purposes, they have received little attention for what they may indicate about the history and properties of the flow itself. In contrast, lateral levees along lava flows have long been used by physical volcanologists as tools to learn about their non-Newtonian rheologies and chemical compositions. Hulme (1974) was the first to note that, for a given slope, levee width may be characteristic of a fluids's yield strength and his methodology has been subsequently used to infer properties of lavas on the Earth, the Moon, and Mars. Using these lavas as analogies, we apply Hulme's approach to earthflows in a variety of settings globally. We find that calculated yield strengths for individual earthflows fall within a relatively narrow range between 101-102 kPa. In addition, individual earthflow complexes often preserve multiple generations of levees, which in some cases may record apparent reductions in yield strength over time for a given flow, possibly from weakening of previously failed material. Knowledge of earthflow yield strength permits the calculation of a critical earthflow thickness below which there will be no downslope motion for a given slope angle. Thicknesses calculated in this manner could thus be used to estimate the flux of landslide material for earthflows without direct depth constraints, provided that surface velocity measurements are obtained by other methods (e.g. InSAR, GPS, manual feature tracking).

The influence of lateral forces on the cell stiffness measurement by optical tweezers vertical indentation

NASA Astrophysics Data System (ADS)

Ndoye, Fatou; Sulaiman Yousafzai, Muhammad; Coceano, Giovanna; Bonin, Serena; Scoles, Giacinto; Ka, Oumar; Niemela, Joseph; Cojoc, Dan

2016-01-01

We studied the lateral forces arising during the vertical indentation of the cell membrane by an optically trapped microbead, using back focal plane interferometry to determine force components in all directions. We analyzed the cell-microbead interaction and showed that indeed the force had also lateral components. Using the Hertz model, we calculated and compared the elastic moduli resulting from the total and vertical forces, showing that the differences are important and the total force should be considered. To confirm our results we analyzed cells from two breast cancer cell lines: MDA-MB-231 and HBL-100, known to have different cancer aggressiveness and hence stiffness.

Lateral unicompartmental knee replacement: fixed or mobile bearing?

PubMed

Forster, M C; Bauze, A J; Keene, G C R

2007-09-01

Between May 2001 and May 2003, 233 consecutive Preservation unicompartmental knee replacements (UKR) were performed. Of these, 30 were lateral UKRs (13%) performed in 12 men and 16 women (2 bilateral cases) with a mean age of 67 years (range 36-93 years). A metal-backed mobile bearing tibial component was used in 13 knees and an all-polyethylene fixed bearing tibial component in 17 knees. The patients were reviewed prospectively at 1 and 2 years. The 2 year results show no difference in range of motion or function between the mobile and fixed bearing versions of the Preservation knee when used on the lateral side. There were three early revisions, all in the mobile bearing group.

Magnetohydrodynamic flow and heat transfer around a heated cylinder of arbitrary conductivity

NASA Astrophysics Data System (ADS)

Tassone, A.; Nobili, M.; Caruso, G.

2017-11-01

The interaction of the liquid metal with the plasma confinement magnetic field constitutes a challenge for the design of fusion reactor blankets, due to the arise of MHD effects: increased pressure drops, heat transfer suppression, etc. To overcome these issues, a dielectric fluid can be employed as coolant for the breeding zone. A typical configuration involves pipes transverse to the liquid metal flow direction. This numerical study is conducted to assess the influence of pipe conductivity on the MHD flow and heat transfer. The CFD code ANSYS CFX was employed for this purpose. The fluid is assumed to be bounded by rectangular walls with non-uniform thickness and subject to a skewed magnetic field with the main component aligned with the cylinder axis. The simulations were restricted to Re = (20; 40) and M = (10; 50). Three different scenarios for the obstacle were considered: perfectly insulating, finite conductivity and perfectly conducting. The electrical conductivity was found to affect the channel pressure penalty due to the obstacle insertion only for M = 10 and just for the two limiting cases. A general increment of the heat transfer with M was found due to the tendency of the magnetic field to equalize the flow rate between the sub-channels individuated by the pipe. The best results were obtained with the insulating pipe, due to the reduced electromagnetic drag. The generation of counter-rotating vortices close to the lateral duct walls was observed for M = 50 and perfectly conducting pipe as a result of the modified currents distribution.

Regimes of plume-slab interaction and consequences for hotspot volcanism

NASA Astrophysics Data System (ADS)

Druken, Kelsey; Stegman, Dave; Kincaid, Christopher; Griffiths, Ross

2013-04-01

"Hotspot" volcanism is generally attributed to upwelling of anomalously warm mantle plumes, the intra-plate Hawaiian island chain and its simple age progression serving as an archetypal example. However, interactions of such plumes with plate margins, and in particular with subduction zones, is likely to have been a common occurrence and leads to more complicated geological records. Here we present results from a series of complementary, three-dimensional numerical and laboratory experiments that examine the dynamic interaction between negatively buoyant subducting slabs and positively buoyant mantle plumes. Slab-driven flow is shown to significantly influence the evolution and morphology of nearby plumes, which leads to a range of deformation regimes of the plume head and conduit. The success or failure of an ascending plume head to reach the lithosphere depends on the combination of plume buoyancy and position within the subduction system, where the mantle flow owing to downdip and rollback components of slab motion entrain plume material both vertically and laterally. Plumes rising within the sub-slab region tend to be suppressed by the surrounding flow field, while wedge-side plumes experience a slight enhancement before ultimately being entrained by subduction. Hotspot motion is more complex than that expected at intraplate settings and is primarily controlled by position alone. Regimes include severely deflected conduits as well as retrograde (corkscrew) motion from rollback-driven flow, often with weak and variable age-progression. The interaction styles and surface manifestations of plumes can be predicted from these models, and the results have important implications for potential hotspot evolution near convergent margins.

Subduction disfigured mantle plumes: Plumes that are not plumes?

NASA Astrophysics Data System (ADS)

Druken, K. A.; Stegman, D. R.; Kincaid, C. R.; Griffiths, R. W.

2012-12-01

"Hotspot" volcanism is generally attributed to upwelling of anomalously warm mantle plumes, the intra-plate Hawaiian island chain and its simple age progression serving as an archetypal example. However, interactions of such plumes with plate margins, and in particular with subduction zones, is likely to have been a common occurrence and leads to more complicated geological records. Here we present results from a series of complementary, three-dimensional numerical and laboratory experiments that examine the dynamic interaction between negatively buoyant subducting slabs and positively buoyant mantle plumes. Slab-driven flow is shown to significantly influence the evolution and morphology of nearby plumes, which leads to a range of deformation regimes of the plume head and conduit. The success or failure of an ascending plume head to reach the lithosphere depends on the combination of plume buoyancy and position within the subduction system, where the mantle flow owing to downdip and rollback components of slab motion entrain plume material both vertically and laterally. Plumes rising within the sub-slab region tend to be suppressed by the surrounding flow field, while wedge-side plumes experience a slight enhancement before ultimately being entrained by subduction. Hotspot motion is more complex than that expected at intraplate settings and is primarily controlled by position alone. Regimes include severely deflected conduits as well as retrograde (corkscrew) motion from rollback-driven flow, often with weak and variable age-progression. The interaction styles and surface manifestations of plumes can be predicted from these models, and the results have important implications for potential hotspot evolution near convergent margins.

A geometry package for generation of input data for a three-dimensional potential-flow program

NASA Technical Reports Server (NTRS)

Halsey, N. D.; Hess, J. L.

1978-01-01

The preparation of geometric data for input to three-dimensional potential flow programs was automated and simplified by a geometry package incorporated into the NASA Langley version of the 3-D lifting potential flow program. Input to the computer program for the geometry package consists of a very sparse set of coordinate data, often with an order of magnitude of fewer points than required for the actual potential flow calculations. Isolated components, such as wings, fuselages, etc. are paneled automatically, using one of several possible element distribution algorithms. Curves of intersection between components are calculated, using a hybrid curve-fit/surface-fit approach. Intersecting components are repaneled so that adjacent elements on either side of the intersection curves line up in a satisfactory manner for the potential-flow calculations. Many cases may be run completely (from input, through the geometry package, and through the flow calculations) without interruption. Use of the package significantly reduces the time and expense involved in making three-dimensional potential flow calculations.

The mechanism by which nonlinearity sustains turbulence in plane Couette flow

NASA Astrophysics Data System (ADS)

Nikolaidis, M.-A.; Farrell, B. F.; Ioannou, P. J.

2018-04-01

Turbulence in wall-bounded shear flow results from a synergistic interaction between linear non-normality and nonlinearity in which non-normal growth of a subset of perturbations configured to transfer energy from the externally forced component of the turbulent state to the perturbation component maintains the perturbation energy, while the subset of energy-transferring perturbations is replenished by nonlinearity. Although it is accepted that both linear non-normality mediated energy transfer from the forced component of the mean flow and nonlinear interactions among perturbations are required to maintain the turbulent state, the detailed physical mechanism by which these processes interact in maintaining turbulence has not been determined. In this work a statistical state dynamics based analysis is performed on turbulent Couette flow at R = 600 and a comparison to DNS is used to demonstrate that the perturbation component in Couette flow turbulence is replenished by a non-normality mediated parametric growth process in which the fluctuating streamwise mean flow has been adjusted to marginal Lyapunov stability. It is further shown that the alternative mechanism in which the subspace of non-normally growing perturbations is maintained directly by perturbation-perturbation nonlinearity does not contribute to maintaining the turbulent state. This work identifies parametric interaction between the fluctuating streamwise mean flow and the streamwise varying perturbations to be the mechanism of the nonlinear interaction maintaining the perturbation component of the turbulent state, and identifies the associated Lyapunov vectors with positive energetics as the structures of the perturbation subspace supporting the turbulence.

Estimating Vibrational Powers Of Parts In Fluid Machinery

NASA Technical Reports Server (NTRS)

Harvey, S. A.; Kwok, L. C.

1995-01-01

In new method of estimating vibrational power associated with component of fluid-machinery system, physics of flow through (or in vicinity of) component regarded as governing vibrations. Devised to generate scaling estimates for design of new parts of rocket engines (e.g., pumps, combustors, nozzles) but applicable to terrestrial pumps, turbines, and other machinery in which turbulent flows and vibrations caused by such flows are significant. Validity of method depends on assumption that fluid flows quasi-steadily and that flow gives rise to uncorrelated acoustic powers in different parts of pump.

Alpha-defensin and the Synovasure lateral flow device for the diagnosis of prosthetic joint infection.

PubMed

Marson, B A; Deshmukh, S R; Grindlay, D J C; Scammell, B E

2018-06-01

Aims The aim of this review was to evaluate the available literature and to calculate the pooled sensitivity and specificity for the different alpha-defensin test systems that may be used to diagnose prosthetic joint infection (PJI). Materials and Methods Studies using alpha-defensin or Synovasure (Zimmer Biomet, Warsaw, Indiana) to diagnose PJI were identified from systematic searches of electronic databases. The quality of the studies was evaluated using the Quality Assessment of Studies of Diagnostic Accuracy (QUADAS) tool. Meta-analysis was completed using a bivariate model. Results A total of 11 eligible studies were included. The median QUADAS score was 13 (interquartile range 13 to 13) out of 14. Significant conflicts of interest were identified in five studies. The pooled sensitivity for the laboratory alpha-defensin test was 0.95 (95% confidence interval (CI) 0.91 to 0.98) and the pooled specificity was 0.97 (95% CI 0.95 to 0.98) for four studies with a threshold level of 5.2 mgl -1 The pooled sensitivity for the lateral flow cassette test was 0.85 (95% CI 0.74 to 0.92) and the pooled specificity was 0.90 (95% CI 0.91 to 0.98). There was a statistically significant difference in sensitivity (p = 0.019), but not specificity (p = 0.47). Conclusion Laboratory-based alpha-defensin testing remains a promising tool for diagnosing PJI. The lateral flow cassette has a significantly lower performance and pooled results are comparable to the leucocyte esterase test. Further studies are required before the widespread adoption of the lateral flow cassette alpha-defensin test. Cite this article: Bone Joint J 2018;100-B:703-11.

Influence of gas flow and applied voltage on interaction of jets in a cross-field helium plasma jet array

NASA Astrophysics Data System (ADS)

Wan, Meng; Liu, Feng; Fang, Zhi; Zhang, Bo; Wan, Hui

2017-09-01

Atmospheric Pressure Plasma Jet arrays can greatly enhance the treatment area to fulfill the need for large-scale surface processing, while the spatial uniformity of the plasma jet array is closely related to the interactions of the adjacent jets. In this paper, a three-tube one-dimensional (1D) He plasma jet array with a cross-field needle-ring electrode structure is used to investigate the influences of the gas flow rate and applied voltage on the interactions of the adjacent jets through electrical, optical, and fluid measurements. The repulsion of the adjacent plume channels is observed using an intensified charge-coupled device (ICCD) and the influence of the gas flow rate and applied voltage on the electrostatic repulsion force, Coulomb force, is discussed. It is found that electrical coupling, mainly electrostatic repulsion force, exists among the jets in the array, which causes both the divergence of the lateral plumes and the nonlinear changes of the discharge power and the transport charge. The deflection angle of the lateral plumes with respect to the central plume in the optical images increases with the increase of applied voltage and decreases with the increase of gas flow rate. The deflection angle of the lateral plumes in the optical images is obviously larger than that of the lateral gas streams in the Schlieren images under the same experimental conditions, and the unconformity of the deflection angles is mainly attributed to the electrostatic repulsion force in adjacent plasma plume channels. The experimental results can help understand the interaction mechanisms of jets in the array and design controllable and scalable plasma jet arrays.

Evaluation of a new nanoparticle-based lateral-flow immunoassay for the exclusion of heparin-induced thrombocytopenia (HIT).

PubMed

Sachs, Ulrich J; von Hesberg, Jakob; Santoso, Sentot; Bein, Gregor; Bakchoul, Tamam

2011-12-01

Heparin-induced thrombocytopenia (HIT) is an adverse complication of heparin caused by HIT antibodies (abs) that recognise platelet factor 4-heparin (PF4/hep) complexes. Several laboratory tests are available for the confirmation and/or refutation of HIT. A reliable and rapid single-sample test is still pending. It was the objective of this study to evaluate a new lateral-flow immunoassay based on nanoparticle technology. A cohort of 452 surgical and medical patients suspected of having HIT was evaluated. All samples were tested in two IgG-specific ELISAs, in a particle gel immunoassay (PaGIA) and in a newly developed lateral-flow immunoassay (LFI-HIT) as well as in a functional test (HIPA). Clinical pre-test probability was determined using 4T's score. Platelet-activating antibodies were present in 34/452 patients, all of whom had intermediate to high clinical probability. PF4/hep abs were detected in 79, 87, 86, and 63 sera using the four different immunoassays. The negative predictive values (NPV) were 100% for both ELISA tests and LFI-HIT but only 99.2% for PaGIA. There were less false positives (n=29) in the LFI-HIT compared to any other test. Additionally, significantly less time was required to perform LFI-HIT than to perform the other immunoassays. In conclusion, a newly developed lateral-flow assay, LFI-HIT, was capable of identifying all HIT patients in a cohort in a short period of time. Beside an NPV of 100%, the rate of false-positive signals is significantly lower with LFI-HIT than with other immunoassay(s). These performance characteristics suggest a high potency in reducing the risk and costs in patients suspected of having HIT.

CdSe/ZnS Quantum Dot-Labeled Lateral Flow Strips for Rapid and Quantitative Detection of Gastric Cancer Carbohydrate Antigen 72-4

NASA Astrophysics Data System (ADS)

Yan, Xinyu; Wang, Kan; Lu, Wenting; Qin, Weijian; Cui, Daxiang; He, Jinghua

2016-03-01

Carbohydrate antigen 72-4 (CA72-4) is an important biomarker associated closely with diagnosis and prognosis of early gastric cancer. How to realize quick, sensitive, specific, and quantitative detection of CA72-4 in clinical specimens has become a great requirement. Herein, we reported a CdSe/ZnS quantum dot-labeled lateral flow test strip combined with a charge-coupled device (CCD)-based reader was developed for rapid, sensitive, and quantitative detection of CA72-4. Two mouse monoclonal antibodies (mAbs) against CA72-4 were employed. One of them was coated as a test line, while another mAb was labeled with quantum dots and coated onto conjugate pad. The goat anti-mouse IgG was immobilized as a control line. After sample was added, a sandwich structure was formed with CA72-4 and these two mAbs. The fluorescent signal from quantum dots (QD)-labeled mAb in sandwich structure was related to the amount of detected CA72-4. A CCD-based reader was used to realize quantitative detection of CA72-4. Results showed that developed QD-labeled lateral flow strips to detect CA72-4 biomarker with the sensitivity of 2 IU/mL and 10 min detection time. One hundred sera samples from clinical patients with gastric cancer and healthy people were used to confirm specificity of this strip method; results showed that established strip method own 100 % reproducibility and 100 % specificity compared with Roche electrochemiluminescence assay results. In conclusion, CdSe/ZnS quantum dot-labeled lateral flow strips for detection of CA72-4 could realize rapid, sensitive, and specific detection of clinical samples and could own great potential in clinical translation in near future.

Quantitative measurement of thin phase objects: comparison of speckle deflectometry and defocus-variant lateral shear interferometry.

PubMed

Sjodahl, Mikael; Amer, Eynas

2018-05-10

The two techniques of lateral shear interferometry and speckle deflectometry are analyzed in a common optical system for their ability to measure phase gradient fields of a thin phase object. The optical system is designed to introduce a shear in the frequency domain of a telecentric imaging system that gives a sensitivity of both techniques in proportion to the defocus introduced. In this implementation, both techniques successfully measure the horizontal component of the phase gradient field. The response of both techniques scales linearly with the defocus distance, and the precision is comparative, with a random error in the order of a few rad/mm. It is further concluded that the precision of the two techniques relates to the transverse speckle size in opposite ways. While a large spatial coherence width, and correspondingly a large lateral speckle size, makes lateral shear interferometry less susceptible to defocus, a large lateral speckle size is detrimental for speckle correlation. The susceptibility for the magnitude of the defocus is larger for the lateral shear interferometry technique as compared to the speckle deflectometry technique. The two techniques provide the same type of information; however, there are a few fundamental differences. Lateral shear interferometry relies on a special hardware configuration in which the shear angle is intrinsically integrated into the system. The design of a system sensitive to both in-plane phase gradient components requires a more complex configuration and is not considered in this paper. Speckle deflectometry, on the other hand, requires no special hardware, and both components of the phase gradient field are given directly from the measured speckle deformation field.

The Natural Helmholtz-Hodge Decomposition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bhatia, H.

nHHD is a C++ library to decompose a flow field into three components exhibiting specific types of behaviors. These components allow more targeted analysis of flow behavior and can be applied to a variety of application areas.

Should the position of the patellar component replicate the vertical median ridge of the native patella?

PubMed

Lee, Rae Hyeong; Jeong, Hae Won; Lee, Jin Kyu; Choi, Choong Hyeok

2017-01-01

In total knee arthroplasty (TKA), the position of the patellar component can affect patellar tracking. However, the patellar component cannot always replicate the original high point of the patella because of anatomical variance. This study investigated whether altering the highest point of the patella can affect outcomes of primary TKA, especially in patients having a patella with a far-medialized median ridge. A retrospective review was performed for 177 knees (143 patients) treated with primary TKA between July 2011 and March 2014. Group 1 (34 knees) had the patellar component displaced over three millimeters from the median ridge, while Group 2 (143 knees) had the patellar component placed on the original median ridge position. The one-year follow-up outcomes were reviewed, including: patellar tilt angle, Knee Society Score, Feller Patellar Score, and modified Kujala Anterior Knee Pain Score. Mean (±standard deviation) displacement of the patellar component in Group 1 was 3.97±0.97mm lateral to the original position of the median ridge, with a significant decrease in lateral patellar tilt angle (P<0.001). Lateral patellar tilt showed a positive correlation with the medialization of the patellar component (P<0.001, r=0.401). Ability to rise from a chair was better in Group 1 (P=0.025). There were no other between-group differences in other clinical outcomes. There should be no need for the patellar component to replicate the original highest point of the native patella in primary TKA. Copyright © 2016 Elsevier B.V. All rights reserved.

Mobility power flow analysis of an L-shaped plate structure subjected to acoustic excitation

NASA Technical Reports Server (NTRS)

Cuschieri, J. M.

1989-01-01

An analytical investigation based on the Mobility Power Flow method is presented for the determination of the vibrational response and power flow for two coupled flat plate structures in an L-shaped configuration, subjected to acoustical excitation. The principle of the mobility power flow method consists of dividing the global structure into a series of subsystems coupled together using mobility functions. Each separate subsystem is analyzed independently to determine the structural mobility functions for the junction and excitation locations. The mobility functions, together with the characteristics of the junction between the subsystems, are then used to determine the response of the global structure and the power flow. In the coupled plate structure considered here, mobility power flow expressions are derived for excitation by an incident acoustic plane wave. In this case, the forces (acoustic pressures) acting on the structure are dependent on the response of the structure because of the scattered pressure component. The interaction between the structure and the fluid leads to the derivation of a corrected mode shape for the plates' normal surface velocity and also for the structure mobility functions. The determination of the scattered pressure components in the expressions for the power flow represents an additional component in the power flow balance for the source plate and the receiver plate. This component represents the radiated acoustical power from the plate structure.

Electric analog of three-dimensional flow to wells and its application to unconfined aquifers

USGS Publications Warehouse

Stallman, Robert W.

1963-01-01

Electric-analog design criteria are established from the differential equations of ground-water flow for analyzing pumping-test data. A convenient analog design was obtained by transforming the cylindrical equation of flow to a rectilinear form. The design criteria were applied in the construction of an electric analog, which was used for studying pumping-test data collected near Grand Island, Nebr. Data analysis indicated (1) vertical flow components near pumping wells in unconfined aquifers may be much more significant in the control of water-table decline than radial flow components for as much as a day of pumping; (2) the specific yield during the first few minutes of pumping appears to be a very small fraction of that observed after pumping for more than 1 day; and (3) estimates of specific yield made from model studies seem much more sensitive to variations in assumed flow conditions than are estimates of permeability. Analysis of pumping-test data where vertical flow components are important requires that the degree of anisotropy be known. A procedure for computing anisotropy directly from drawdowns observed at five points was developed. Results obtained in the analog study emphasize the futility of calculating unconfined aquifer properties from pumping tests of short duration by means of equations based on the assumptions that vertical flow components are negligible and specific yield is constant.

Inferring common cognitive mechanisms from brain blood-flow lateralization data: a new methodology for fTCD analysis.

PubMed

Meyer, Georg F; Spray, Amy; Fairlie, Jo E; Uomini, Natalie T

2014-01-01

Current neuroimaging techniques with high spatial resolution constrain participant motion so that many natural tasks cannot be carried out. The aim of this paper is to show how a time-locked correlation-analysis of cerebral blood flow velocity (CBFV) lateralization data, obtained with functional TransCranial Doppler (fTCD) ultrasound, can be used to infer cerebral activation patterns across tasks. In a first experiment we demonstrate that the proposed analysis method results in data that are comparable with the standard Lateralization Index (LI) for within-task comparisons of CBFV patterns, recorded during cued word generation (CWG) at two difficulty levels. In the main experiment we demonstrate that the proposed analysis method shows correlated blood-flow patterns for two different cognitive tasks that are known to draw on common brain areas, CWG, and Music Synthesis. We show that CBFV patterns for Music and CWG are correlated only for participants with prior musical training. CBFV patterns for tasks that draw on distinct brain areas, the Tower of London and CWG, are not correlated. The proposed methodology extends conventional fTCD analysis by including temporal information in the analysis of cerebral blood-flow patterns to provide a robust, non-invasive method to infer whether common brain areas are used in different cognitive tasks. It complements conventional high resolution imaging techniques.

Overpressure, Flow Focusing, Compaction and Slope Stability on the continental slope: Insights from IODP Expedition 308

NASA Astrophysics Data System (ADS)

Flemings, P. B.

2010-12-01

Integrated Ocean Drilling Program Expepedition 308 used direct measurements of pore pressure, analysis of hydromechanical properties, and geological analysis to illuminate how sedimentation, flow focusing, overpressure, and slope stability couple beneath the seafloor on the deepwater continental slope in the Gulf of Mexico. We used pore pressure penetrometers to measure severe overpressures (60% of the difference between lithostatic stress and hydrostatic pressure) that extend from the seafloor for 100’s of meters. We ran uniaxial consolidation experiments on whole core and found that although permeability is relatively high near the seafloor, the sediments are highly compressible. As a result, the coefficient of consolidation (the hydraulic diffusivity) is remarkably constant over a large range of effective stresses. This behavior accounts for the high overpressure that begins near the seafloor and extends to depth. Forward modeling suggests that flow is driven laterally along a permeable unit called the Blue Unit. Calculations suggest that soon after deposition, lateral flow lowered the effective stress and triggered the submarine landslides that we observe. Later in the evolution of this system, overpressure may have pre-conditioned the slope to failure by earthquakes. Results from IODP Expedition 308 illustrate how pore pressure and sedimentation control the large-scale form of continental margins, how submarine landslides form, and provide strategies for designing stable drilling programs.

Increasing Accuracy of Tissue Shear Modulus Reconstruction Using Ultrasonic Strain Tensor Measurement

NASA Astrophysics Data System (ADS)

Sumi, C.

Previously, we developed three displacement vector measurement methods, i.e., the multidimensional cross-spectrum phase gradient method (MCSPGM), the multidimensional autocorrelation method (MAM), and the multidimensional Doppler method (MDM). To increase the accuracies and stabilities of lateral and elevational displacement measurements, we also developed spatially variant, displacement component-dependent regularization. In particular, the regularization of only the lateral/elevational displacements is advantageous for the lateral unmodulated case. The demonstrated measurements of the displacement vector distributions in experiments using an inhomogeneous shear modulus agar phantom confirm that displacement-component-dependent regularization enables more stable shear modulus reconstruction. In this report, we also review our developed lateral modulation methods that use Parabolic functions, Hanning windows, and Gaussian functions in the apodization function and the optimized apodization function that realizes the designed point spread function (PSF). The modulations significantly increase the accuracy of the strain tensor measurement and shear modulus reconstruction (demonstrated using an agar phantom).

Three-dimensional circulation dynamics of along-channel flow in stratified estuaries

NASA Astrophysics Data System (ADS)

Musiak, Jeffery Daniel

Estuaries are vital because they are the major interface between humans and the oceans and provide valuable habitat for a wide range of organisms. Therefore it is important to model estuarine circulation to gain a better comprehension of the mechanics involved and how people effect estuaries. To this end, this dissertation combines analysis of data collected in the Columbia River estuary (CRE) with novel data processing and modeling techniques to further the understanding of estuaries that are strongly forced by riverflow and tides. The primary hypothesis tested in this work is that the three- dimensional (3-D) variability in along-channel currents in a strongly forced estuary can be largely accounted for by including the lateral variations in density and bathymetry but neglecting the secondary, or lateral, flow. Of course, the forcing must also include riverflow and oceanic tides. Incorporating this simplification and the modeling ideas put forth by others with new modeling techniques and new ideas on estuarine circulation will allow me to create a semi-analytical quasi 3-D profile model. This approach was chosen because it is of intermediate complexity to purely analytical models, that, if tractable, are too simple to be useful, and 3-D numerical models which can have excellent resolution but require large amounts of time, computer memory and computing power. Validation of the model will be accomplished using velocity and density data collected in the Columbia River Estuary and by comparison to analytical solutions. Components of the modeling developed here include: (1) development of a 1-D barotropic model for tidal wave propagation in frictionally dominated systems with strong topography. This model can have multiple tidal constituents and multiply connected channels. (2) Development and verification of a new quasi 3-D semi-analytical velocity profile model applicable to estuarine systems which are strongly forced by both oceanic tides and riverflow. This model includes diurnal and semi-diurnal tidal and non- linearly generated overtide circulation and residual circulation driven by riverflow, baroclinic forcing, surface wind stress and non-linear tidal forcing. (3) Demonstration that much of the lateral variation in along-channel currents is caused by variations in along- channel density forcing and bathymetry.

An Equation Governing Ultralow-Velocity Zones: Implications for Holes in the ULVZ, Lateral Chemical Reactions at the Core-Mantle Boundary, and Damping of Heat Flux Variations in the Core

NASA Astrophysics Data System (ADS)

Hernlund, J. W.; Matsui, H.

2017-12-01

Ultralow-velocity zones (ULVZ) are increasingly illuminated by seismology, revealing surprising diversity in size, shape, and physical characteristics. The only viable hypotheses are that ULVZs are a compositionally distinct FeO-enriched dense material, which could have formed by fractional crystallization of a basal magma ocean, segregation of subducted banded iron formations, precipitation of solids from the outer core, partial melting and segregation of iron-rich melts from subducted basalts, or most likely a combination of many different processes. But many questions remain: Are ULVZ partially molten in some places, and not in others? Are ULVZ simply the thicker portions of an otherwise global thin layer, covering the entire CMB and thus blocking or moderating chemical interactions between the core and overlying mantle? Is such a layer inter-connected and able to conduct electrical currents that allow electro-magnetic coupling of core and mantle angular momentum? Are they being eroded and shrinking in size due to viscous entrainment, or is more material being added to ULVZ over time? Here we derive an advection-diffusion-like equation that governs the dynamical evolution of a chemically distinct ULVZ. Analysis of this equation shows that ULVZ should become readily swept aside by viscous mantle flows at the CMB, exposing "ordinary mantle" to the top of the core, thus inducing chemical heterogeneity that drives lateral CMB chemical reactions. These reactions are correlated with heat flux, thus maintaining large-scale pressure variations atop the core that induce cyclone-like flows centered around ULVZ and ponded subducted slabs. We suggest that turbulent diffusion across adjacent cyclone streams inside a stratified region atop the core readily accommodates lateral transport and re-distribution of components such as O and Si, in addition to heat. Our model implies that the deeper core is at least partly shielded from the influence of strong heat flux variations at the CMB which might otherwise cause problems for producing a geodynamo.

Investigation of spiral blood flow in a model of arterial stenosis.

PubMed

Paul, Manosh C; Larman, Arkaitz

2009-11-01

The spiral component of blood flow has both beneficial and detrimental effects in human circulatory system [Stonebridge PA, Brophy CM. Spiral laminar flow in arteries? Lancet 1991; 338: 1360-1]. We investigate the effects of the spiral blood flow in a model of three-dimensional arterial stenosis with a 75% cross-sectional area reduction at the centre by means of computational fluid dynamics (CFD) techniques. The standard k-omega model is employed for simulation of the blood flow for the Reynolds number of 500 and 1000. We find that for Re=500 the spiral component of the blood flow increases both the total pressure and velocity of the blood, and some significant differences are found between the wall shear stresses of the spiral and non-spiral induced flow downstream of the stenosis. The turbulent kinetic energy is reduced by the spiral flow as it induces the rotational stabilities in the forward flow. For Re=1000 the tangential component of the blood velocity is most influenced by the spiral speed, but the effect of the spiral flow on the centreline turbulent kinetic energy and shear stress is mild. The results of the effects of the spiral flow are discussed in the paper along with the relevant pathological issues.

Value for controlling flow of cryogenic fluid

DOEpatents

Knapp, Philip A.

1996-01-01

A valve is provided for accurately controlling the flow of cryogenic fluids such as liquid nitrogen. The valve comprises a combination of disc and needle valves affixed to a valve stem in such a manner that the disc and needle are free to rotate about the stem, but are constrained in lateral and vertical movements. This arrangement provides accurate and precise fluid flow control and positive fluid isolation.

Regimes of Two-Phase Flow in Short Rectangular Channel

NASA Astrophysics Data System (ADS)

Chinnov, Evgeny A.; Guzanov, Vladimir V.; Cheverda, Vyacheslav; Markovich, Dmitry M.; Kabov, Oleg A.

2009-08-01

Experimental study of two-phase flow in the short rectangular horizontal channel with height 440 μm has been performed. Characteristics of liquid motion inside the channel have been registered and measured by the Laser Induced Fluorescence technique. New information has allowed determining more precisely the characteristics of churn regime and boundaries between different regimes of two-phase flow. It was shown that formation of some two-phase flow regimes and transitions between them are determined by instability of the flow in the lateral parts of the channel.

Shaft Seal Compensates for Cold Flow

NASA Technical Reports Server (NTRS)

Myers, W. N.; Hein, L. A.

1985-01-01

Seal components easy to install. Ring seal for rotating or reciprocating shafts spring-loaded to compensate for slow yielding (cold flow) of sealing material. New seal relatively easy to install because components preassembled, then installed in one piece.

Elevations, Major Component Isometric, Propellant Flow Schematic, and External Tank ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Elevations, Major Component Isometric, Propellant Flow Schematic, and External Tank Connection to Shuttle Main Engines - Space Transportation System, Space Shuttle Main Engine, Lyndon B. Johnson Space Center, 2101 NASA Parkway, Houston, Harris County, TX

Measurements of the wall-normal velocity component in very high Reynolds number pipe flow

NASA Astrophysics Data System (ADS)

Vallikivi, Margit; Hultmark, Marcus; Smits, Alexander J.

2012-11-01

Nano-Scale Thermal Anemometry Probes (NSTAPs) have recently been developed and used to study the scaling of the streamwise component of turbulence in pipe flow over a very large range of Reynolds numbers. This probe has an order of magnitude higher spatial and temporal resolution than regular hot wires, allowing it to resolve small scale motions at very high Reynolds numbers. Here use a single inclined NSTAP probe to study the scaling of the wall normal component of velocity fluctuations in the same flow. These new probes are calibrated using a method that is based on the use of the linear stress region of a fully developed pipe flow. Results on the behavior of the wall-normal component of velocity for Reynolds numbers up to 2 million are reported. Supported under NR Grant N00014-09-1-0263 (program manager Ron Joslin) and NSF Grant CBET-1064257 (program manager Henning Winter).

Thermal effects of groundwater flow through subarctic fens: A case study based on field observations and numerical modeling

DOE PAGES

Sjöberg, Ylva; Coon, Ethan; K. Sannel, A. Britta; ...

2016-02-04

Modeling and observation of ground temperature dynamics are the main tools for understanding current permafrost thermal regimes and projecting future thaw. Until recently, most studies on permafrost have focused on vertical ground heat fluxes. Groundwater can transport heat in both lateral and vertical directions but its influence on ground temperatures at local scales in permafrost environments is not well understood. In this paper, we combine field observations from a subarctic fen in the sporadic permafrost zone with numerical simulations of coupled water and thermal fluxes. At the Tavvavuoma study site in northern Sweden, ground temperature profiles and groundwater levels weremore » observed in boreholes. These observations were used to set up one- and two-dimensional simulations down to 2 m depth across a gradient of permafrost conditions within and surrounding the fen. Two-dimensional scenarios representing the fen under various hydraulic gradients were developed to quantify the influence of groundwater flow on ground temperature. Our observations suggest that lateral groundwater flow significantly affects ground temperatures. This is corroborated by modeling results that show seasonal ground ice melts 1 month earlier when a lateral groundwater flux is present. Further, although the thermal regime may be dominated by vertically conducted heat fluxes during most of the year, isolated high groundwater flow rate events such as the spring freshet are potentially important for ground temperatures. Finally, as sporadic permafrost environments often contain substantial portions of unfrozen ground with active groundwater flow paths, knowledge of this heat transport mechanism is important for understanding permafrost dynamics in these environments.« less

Advanced nozzle and engine components test facility

NASA Technical Reports Server (NTRS)

Beltran, Luis R.; Delroso, Richard L.; Delrosario, Ruben

1992-01-01

A test facility for conducting scaled advanced nozzle and engine component research is described. The CE-22 test facility, located in the Engine Research Building of the NASA Lewis Research Center, contains many systems for the economical testing of advanced scale-model nozzles and engine components. The combustion air and altitude exhaust systems are described. Combustion air can be supplied to a model up to 40 psig for primary air flow, and 40, 125, and 450 psig for secondary air flow. Altitude exhaust can be simulated up to 48,000 ft, or the exhaust can be atmospheric. Descriptions of the multiaxis thrust stand, a color schlieren flow visualization system used for qualitative flow analysis, a labyrinth flow measurement system, a data acquisition system, and auxiliary systems are discussed. Model recommended design information and temperature and pressure instrumentation recommendations are included.

The study of multiphase flow control during odor reproduction

NASA Astrophysics Data System (ADS)

Luo, Dehan; Yu, Hao; Fan, Danjun; He, Meiqiu

2014-04-01

Odor reproduction, is the use of the chemical composition of the basic components of odor recipe, according to a certain proportion, to control the flow of the various components, which make them sufficiently blended to achieve reproduction. In this paper, reproducing method is to find the corresponding liquid flavor, and then based on chemical flavor recipes, using flowmeters to control the chemical composition of the liquid flavor ratio. In the proportional control, the liquid chemical composition is very likely to be volatile, so that the proportional control is multiphase flow control. Measurement of the flow control will directly affect the odor reproducible results. Using electronic nose to obtain reproducible odor data, and then use pattern recognition algorithm to determine reproducible results. The experimental results can be achieved on the process of odor components multiphase flow proportional control parameter adjustment.

Reconstructing Perceived and Retrieved Faces from Activity Patterns in Lateral Parietal Cortex.

PubMed

Lee, Hongmi; Kuhl, Brice A

2016-06-01

Recent findings suggest that the contents of memory encoding and retrieval can be decoded from the angular gyrus (ANG), a subregion of posterior lateral parietal cortex. However, typical decoding approaches provide little insight into the nature of ANG content representations. Here, we tested whether complex, multidimensional stimuli (faces) could be reconstructed from ANG by predicting underlying face components from fMRI activity patterns in humans. Using an approach inspired by computer vision methods for face recognition, we applied principal component analysis to a large set of face images to generate eigenfaces. We then modeled relationships between eigenface values and patterns of fMRI activity. Activity patterns evoked by individual faces were then used to generate predicted eigenface values, which could be transformed into reconstructions of individual faces. We show that visually perceived faces were reliably reconstructed from activity patterns in occipitotemporal cortex and several lateral parietal subregions, including ANG. Subjective assessment of reconstructed faces revealed specific sources of information (e.g., affect and skin color) that were successfully reconstructed in ANG. Strikingly, we also found that a model trained on ANG activity patterns during face perception was able to successfully reconstruct an independent set of face images that were held in memory. Together, these findings provide compelling evidence that ANG forms complex, stimulus-specific representations that are reflected in activity patterns evoked during perception and remembering. Neuroimaging studies have consistently implicated lateral parietal cortex in episodic remembering, but the functional contributions of lateral parietal cortex to memory remain a topic of debate. Here, we used an innovative form of fMRI pattern analysis to test whether lateral parietal cortex actively represents the contents of memory. Using a large set of human face images, we first extracted latent face components (eigenfaces). We then used machine learning algorithms to predict face components from fMRI activity patterns and, ultimately, to reconstruct images of individual faces. We show that activity patterns in a subregion of lateral parietal cortex, the angular gyrus, supported successful reconstruction of perceived and remembered faces, confirming a role for this region in actively representing remembered content. Copyright © 2016 the authors 0270-6474/16/366069-14$15.00/0.

Cerebral Lateralization and General Intelligence: Gender Differences in a Transcranial Doppler Study

ERIC Educational Resources Information Center

Njemanze, P.C.

2005-01-01

The present study evaluated cerebral lateralization during Raven's progressive matrices (RPM) paradigm in female and male subjects. Bilateral simultaneous transcranial Doppler (TCD) ultrasound was used to measure mean blood flow velocities (MBFV) in the right and left middle cerebral arteries (MCAs) in 24 (15 females and 9 males) right-handed…

Behavior of lateral-deformation coefficients during elastoplastic deformation of metals

NASA Astrophysics Data System (ADS)

Zimin, B. A.; Smirnov, I. V.; Sudenkov, Yu. V.

2017-06-01

The results of investigations into variation of the coefficients of lateral deformation (the Poisson ratio) during single-axis tension of samples of steel 12Kh18N10T and St3, titanium VT1, the aluminum alloy D16AM, copper M1, and a magnesium alloy are considered. The technique developed on the basis of the optoacoustic effect and simultaneous measurements of the longitudinal and surface speeds of sound in metallic samples during the tension makes it possible to measure the rates at various stages of the deformation process. The data obtained make it possible to construct the dependences of variation of the lateral-deformation coefficients at all stages of the plastic flow. The correlation of these variations both with known processes of structural reconstructions at various stages of plastic flow and with the process of localization of plastic-shear bands in the aluminum alloy is noted.

Lateral traction of laminar flow between sliding pair with heterogeneous slip/no-slip surface

NASA Astrophysics Data System (ADS)

Wu, Zhenpeng; Zeng, Liangcai; Chen, Xiaolan; Chen, Keying; Ding, Xianzhong

2017-11-01

The problem of shaft axial motion which significantly affects the lubrication performance has been a common phenomenon in journal bearing systems. The existing work involved in the solution of shaft axial motion is also very rare. In this study, we choose to examine the flow between sliding pair in which regard we present a unique heterogeneous surface consisting of a slip zone and a no-slip zone. The results reveal the following points: 1) By appropriately arranging the slip zone to change the angle between the borderline and the moving direction of the upper plate, it is possible to control the direction of the lateral traction in which the liquid film acts on the upper plate. 2) Exponent of the power function of the borderline and aspect ratio of the computational domain are large or small are not conducive to increasing the effect of lateral traction. For the object of this study, the final results of the optimization are shown that the lateral traction can account for 20% of the resistance.

40 CFR 86.078-3 - Abbreviations.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., and for 1985 and Later Model Year New Gasoline Fueled, Natural Gas-Fueled, Liquefied Petroleum Gas... feet per hour. CFV—Critical flow venturi. CFV-CVS—Critical flow venturi—constant volume sampler... pump—constant volume sampler. ppm—parts per million by volume. ppm C—parts per million, carbon. psi...

Double Diffusive Convection in Materials Processing

NASA Technical Reports Server (NTRS)

Ramachandra, Narayanan; Leslie, Fred W.

1999-01-01

A great number of crystals grown in space are plagued by convective motions which contribute to structural flaws. The character of these instabilities is not well understood but is associated with density variations in the presence of residual gravity (g-jitter). As a specific example, past HgCdTe crystal growth space experiments by Lehoczky and co-workers indicate radial compositional asymmetry in the grown crystals. In the case of HgCdTe the rejected component into the melt upon solidification is HgTe which is denser than the melt. The space grown crystals indicate the presence of three dimensional flow with the heavier HgTe-rich material clearly aligned with the residual gravity (0.55-1.55 micro g) vector. This flow stems from double-diffusive convection, namely, thermal and solutal buoyancy driven flow in the melt. The study of double-diffusive convection is multi-faceted and rather vast. In our investigation, we seek to focus on one specific aspect of this discipline that is of direct relevance to materials processing especially crystal growth, namely, the side ways heating regime. This problem has been widely studied, both experimentally and numerically, in the context of solar ponds wherein the system is characterized by a linear salt (solutal) gradient with an imposed lateral temperature gradient. The induced flow instabilities arise from the wide disparity between the fluid thermal diffusivity and the solute diffusivity. The extension of the analysis to practical crystal growth applications has however not been rigorously made and understood. One subtle but important difference in crystal growth systems is the fact that die system solute gradient is non-linear (typically exponential). Besides, the crystal growth problem has the added complexities of solidification, both lateral and longitudinal thermal gradients and segregation phenomena in systems where binary and ternary compounds are being grown. This paper treats the side ways heating problem alone in a model fluid system. Results from detailed numerical calculations, mainly two dimensional are provided. The interactions between a non-linear solute gradient and an imposed transverse thermal gradient are investigated. The buoyancy effects are treated in the traditional Boussinesq approximation and also in a more complete density formulation to address recent concerns of the first approach especially in simulations of the system response in a reduced gravity environment. Detailed flow, temperature and solute field plots along with heat and mass transfer results are presented in the paper. Implications to practical crystal growth systems as discerned from the modeling results are also explored and reported.

Development of an analytical Lagrangian model for passive scalar dispersion in low-wind speed meandering conditions

NASA Astrophysics Data System (ADS)

Stefanello, M. B.; Degrazia, G. A.; Mortarini, L.; Buligon, L.; Maldaner, S.; Carvalho, J. C.; Acevedo, O. C.; Martins, L. G. N.; Anfossi, D.; Buriol, C.; Roberti, D.

2018-02-01

Describing the effects of wind meandering motions on the dispersion of scalars is a challenging task, since this type of flow represents a physical state characterized by multiple scales. In this study, a Lagrangian stochastic diffusion model is derived to describe scalar transport during the horizontal wind meandering phenomenon that occurs within a planetary boundary layer. The model is derived from the linearization of the Langevin equation, and it employs a heuristic functional form that represents the autocorrelation function of meandering motion. The new solutions, which describe the longitudinal and lateral wind components, were used to simulate tracer experiments that were performed in low-wind speed conditions. The results of the comparison indicate that the new model can effectively reproduce the observed concentrations of the contaminants, and therefore, it can satisfactorily describe enhanced dispersion effects due to the presence of meandering.

Lattice Boltzmann Method for Spacecraft Propellant Slosh Simulation

NASA Technical Reports Server (NTRS)

Orr, Jeb S.; Powers, Joseph F.; Yang, Hong Q.

2015-01-01

A scalable computational approach to the simulation of propellant tank sloshing dynamics in microgravity is presented. In this work, we use the lattice Boltzmann equation (LBE) to approximate the behavior of two-phase, single-component isothermal flows at very low Bond numbers. Through the use of a non-ideal gas equation of state and a modified multiple relaxation time (MRT) collision operator, the proposed method can simulate thermodynamically consistent phase transitions at temperatures and density ratios consistent with typical spacecraft cryogenic propellants, for example, liquid oxygen. Determination of the tank forces and moments relies upon the global momentum conservation of the fluid domain, and a parametric wall wetting model allows tuning of the free surface contact angle. Development of the interface is implicit and no interface tracking approach is required. Numerical examples illustrate the method's application to predicting bulk fluid motion including lateral propellant slosh in low-g conditions.

Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line

PubMed Central

Gregorczyk, Karolina P.; Wyżewski, Zbigniew; Szczepanowska, Joanna; Mielcarska, Matylda B.; Bossowska-Nowicka, Magdalena; Gieryńska, Małgorzata; Boratyńska-Jasińska, Anna; Niemiałtowski, Marek G.

2018-01-01

Mitochondria are multifunctional organelles that participate in numerous processes in response to viral infection, but they are also a target for viruses. The aim of this study was to define subcellular events leading to alterations in mitochondrial morphology and function during infection with ectromelia virus (ECTV). We used two different cell lines and a combination of immunofluorescence techniques, confocal and electron microscopy, and flow cytometry to address subcellular changes following infection. Early in infection of L929 fibroblasts and RAW 264.7 macrophages, mitochondria gathered around viral factories. Later, the mitochondrial network became fragmented, forming punctate mitochondria that co-localized with the progeny virions. ECTV-co-localized mitochondria associated with the cytoskeleton components. Mitochondrial membrane potential, mitochondrial fission–fusion, mitochondrial mass, and generation of reactive oxygen species (ROS) were severely altered later in ECTV infection leading to damage of mitochondria. These results suggest an important role of mitochondria in supplying energy for virus replication and morphogenesis. Presumably, mitochondria participate in transport of viral particles inside and outside of the cell and/or they are a source of membranes for viral envelope formation. We speculate that the observed changes in the mitochondrial network organization and physiology in ECTV-infected cells provide suitable conditions for viral replication and morphogenesis. PMID:29772718

Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line.

PubMed

Gregorczyk, Karolina P; Wyżewski, Zbigniew; Szczepanowska, Joanna; Toka, Felix N; Mielcarska, Matylda B; Bossowska-Nowicka, Magdalena; Gieryńska, Małgorzata; Boratyńska-Jasińska, Anna; Struzik, Justyna; Niemiałtowski, Marek G; Szulc-Dąbrowska, Lidia

2018-05-16

Mitochondria are multifunctional organelles that participate in numerous processes in response to viral infection, but they are also a target for viruses. The aim of this study was to define subcellular events leading to alterations in mitochondrial morphology and function during infection with ectromelia virus (ECTV). We used two different cell lines and a combination of immunofluorescence techniques, confocal and electron microscopy, and flow cytometry to address subcellular changes following infection. Early in infection of L929 fibroblasts and RAW 264.7 macrophages, mitochondria gathered around viral factories. Later, the mitochondrial network became fragmented, forming punctate mitochondria that co-localized with the progeny virions. ECTV-co-localized mitochondria associated with the cytoskeleton components. Mitochondrial membrane potential, mitochondrial fission⁻fusion, mitochondrial mass, and generation of reactive oxygen species (ROS) were severely altered later in ECTV infection leading to damage of mitochondria. These results suggest an important role of mitochondria in supplying energy for virus replication and morphogenesis. Presumably, mitochondria participate in transport of viral particles inside and outside of the cell and/or they are a source of membranes for viral envelope formation. We speculate that the observed changes in the mitochondrial network organization and physiology in ECTV-infected cells provide suitable conditions for viral replication and morphogenesis.

Applicability of a diffusion model to lateral transport in the terrestrial and lunar exospheres.

NASA Technical Reports Server (NTRS)

Hodges, R. R., Jr.

1972-01-01

Kinetic theory is used to determine a series expansion of the vertical flux of particles in an exosphere in terms of time and space derivatives of particle concentration, exobase velocity, and temperature. For sufficiently large scale variations of these parameters in time and space, the series can be truncated to a form that is similar to a diffusion equation. Owing to this analogy, it is possible to unite the mathematical description of molecular diffusion, which governs thermospheric flow, and the corresponding exospheric equation by using effective transport coefficients which change smoothly with altitude through the transition from thermosphere to exosphere. A new definition of the exobase for lateral flow emerges from the analogy of exospheric and thermospheric diffusion, as the altitude where the horizontal mean free path length equals the mean horizontal extent of ballistic trajectories of the transported gas, as opposed to the scale height of the dominant gas which determines the exobase for escape. It is shown that the approximation of exospheric lateral flow as a diffusion process is applicable to global scale problems concerning terrestrial helium and heavier gases, and lunar gases heavier than helium.

Impact of roof height non-uniformity on pollutant transport between a street canyon and intersections.

PubMed

Nosek, Štěpán; Kukačka, Libor; Jurčáková, Klára; Kellnerová, Radka; Jaňour, Zbyněk

2017-08-01

This paper presents an extension of our previous wind-tunnel study (Nosek et al., 2016) in which we highlighted the need for investigation of the removal mechanisms of traffic pollution from all openings of a 3D street canyon. The extension represents the pollution flux (turbulent and advective) measurements at the lateral openings of three different 3D street canyons for the winds perpendicular and oblique to the along-canyon axis. The pollution was simulated by emitting a passive gas (ethane) from a homogeneous ground-level line source positioned along the centreline of the investigated street canyons. The street canyons were formed by courtyard-type buildings of two different regular urban-array models. The first model has a uniform building roof height, while the second model has a non-uniform roof height along each building's wall. The mean flow and concentration fields at the canyons' lateral openings confirm the findings of other studies that the buildings' roof-height variability at the intersections plays an important role in the dispersion of the traffic pollutants within the canyons. For the perpendicular wind, the non-uniform roof-height canyon appreciably removes or entrains the pollutant through its lateral openings, contrary to the uniform canyon, where the pollutant was removed primarily through the top. The analysis of the turbulent mass transport revealed that the coherent flow structures of the lateral momentum transport correlate with the ventilation processes at the lateral openings of all studied canyons. These flow structures coincide at the same areas and hence simultaneously transport the pollutant in opposite directions. Copyright © 2017 Elsevier Ltd. All rights reserved.

Quantifying changes in the contribution of upstream snow and glacier melt to downstream low flows in the River Rhine

NASA Astrophysics Data System (ADS)

Stahl, K.; Kohn, I.; Boehm, M.; Seibert, J.; Freudiger, D.; Gerlinger, K.; Weiler, M.

2016-12-01

Low flows impact river ecosystems and impair water use. In the mid- and downstream reaches of one of the largest rivers in Europe, the River Rhine, low flows can threaten a variety of ecosystem services and direct uses. Low flows in summer and fall are sustained by the snow and ice melt contribution from the glacierized mountain headwaters upstream. This study explores changes in the discharge components of rain, snowmelt and ice melt during extreme low flow events from a downstream perspective. Quantification of the discharge components is based on a novel method of runoff component tracking that was implemented into a model chain, consisting of the HBV model, which includes a glacier mass balance model allowing for areal glacier changes, for the headwaters and the distributed hydrological model LARSIM for the remaining Rhine basin. A transient model run at daily resolution was calibrated to glacier volume change, basin-wide snow cover and snow water equivalent and discharge variability at many gauging stations over the period 1901-2006. The analysis of the resulting discharge components revealed that over the course of the 20th Century, the loss of glacier volume and glacier area in the headwaters appears to have compensated an increasingly negative glacier mass balance, resulting in little long-term change to the ice melt component in summer streamflow - thus showing no clear `peak-water' trend. While the glacier ice melt component was less than two percent of the average annual discharge of the mid and lower reaches of the River Rhine, models suggest its fraction was much higher during extreme low flow events. The low flows of the summers of 1921, 1947, and 2003 were comprised of record daily ice melt fractions of more than one fifth of the daily discharge along the mid and lower reaches from Basel to the mouth. A scenario model run with suppressed glacier area change suggests that the ice melt discharge component would have doubled if the same meteorological event as in 2003 had occurred in the early 1900s when glacier areas were still much larger. Impacts on ecology and water use most likely would have also been less severe. The modeled changes in discharge components thus allow a quantification of the low flow hazard that may loom ahead as the glaciers continue to decline.

Anatomic and hemodynamic evaluation of renal venous flow in varicocele formation using color Doppler sonography with emphasis on renal vein entrapment syndrome.

PubMed

Unlu, Murat; Orguc, Sebnem; Serter, Selim; Pekindil, Gokhan; Pabuscu, Yuksel

2007-01-01

To investigate the anatomic and hemodynamic properties of testicular venous drainage and its effects on varicocele formation and reflux using color Doppler ultrasound (US) with emphasis on renal vein entrapment syndrome. Upper abdominal and scrotal US examinations of 35 varicocele patients and 35 healthy male subjects were performed in the supine position during rest, during a Valsalva maneuver and in the erect position. The aortomesenteric angle and distance (AMA and AMD, respectively), peak mean velocities (PVs) and diameters of different segments of renal veins, testicular vein diameters and duration of flow inversion were measured. In the varicocele group, the lateral segment of the left renal vein (LRV) had a larger diameter and slower PV, and the medial segment of the LRV had a smaller diameter and faster PV. The diameter of the dominant draining vein correlated with the PV of the medial and lateral segments of the LRV, whereas there was no correlation between the diameter of the dominant draining vein and the diameters of the right renal vein (RRV) and the lateral segment of the LRV or the PV of the RRV. The duration of flow inversion correlated with the diameter and PV of the medial segment of the LRV. No correlation between the diameters and PVs of the RRV and the lateral segment of the LRV was detected. The decreases in the AMA, AMD, diameter of the medial segment of the LRV and PV of the lateral segment of the LRV, and the increases in the PV of the medial segment of the LRV and the diameter of the lateral segment of the LRV in varicocele patients in all positions suggest the entrapment or impingement of the left renal vein between the aorta and the superior mesenteric artery. This has been defined as the "nutcracker phenomenon", which is known to affect varicocele formation.

Acute Effects of Lateral Thigh Foam Rolling on Arterial Tissue Perfusion Determined by Spectral Doppler and Power Doppler Ultrasound.

PubMed

Hotfiel, Thilo; Swoboda, Bernd; Krinner, Sebastian; Grim, Casper; Engelhardt, Martin; Uder, Michael; Heiss, Rafael U

2017-04-01

Hotfiel, T, Swoboda, B, Krinner, S, Grim, C, Engelhardt, M, Uder, M, and Heiss, R. Acute effects of lateral thigh foam rolling on arterial tissue perfusion determined by spectral Doppler and power Doppler ultrasound. J Strength Cond Res 31(4): 893-900, 2017-Foam rolling has been developed as a popular intervention in training and rehabilitation. However, evidence on its effects on the cellular and physiological level is lacking. The aim of this study was to assess the effect of foam rolling on arterial blood flow of the lateral thigh. Twenty-one healthy participants (age, 25 ± 2 years; height, 177 ± 9 cm; body weight, 74 ± 9 kg) were recruited from the medical and sports faculty. Arterial tissue perfusion was determined by spectral Doppler and power Doppler ultrasound, represented as peak flow (Vmax), time average velocity maximum (TAMx), time average velocity mean (TAMn), and resistive index (RI), and with semiquantitative grading that was assessed by 4 blindfolded investigators. Measurement values were assessed under resting conditions and twice after foam rolling exercises of the lateral thigh (0 and 30 minutes after intervention). The trochanteric region, mid portion, and distal tibial insertion of the lateral thigh were representative for data analysis. Arterial blood flow of the lateral thigh increased significantly after foam rolling exercises compared with baseline (p ≤ 0.05). We detected a relative increase in Vmax of 73.6% (0 minutes) and 52.7% (30 minutes) (p < 0.001), in TAMx of 53.2% (p < 0.001) and 38.3% (p = 0.002), and in TAMn of 84.4% (p < 0.001) and 68.2% (p < 0.001). Semiquantitative power Doppler scores at all portions revealed increased average grading of 1.96 after intervention and 2.04 after 30 minutes compared with 0.75 at baseline. Our results may contribute to the understanding of local physiological reactions to self-myofascial release.

Hydrologic flow path development varies by aspect during spring snowmelt in complex subalpine terrain

NASA Astrophysics Data System (ADS)

Webb, Ryan W.; Fassnacht, Steven R.; Gooseff, Michael N.

2018-01-01

In many mountainous regions around the world, snow and soil moisture are key components of the hydrologic cycle. Preferential flow paths of snowmelt water through snow have been known to occur for years with few studies observing the effect on soil moisture. In this study, statistical analysis of the topographical and hydrological controls on the spatiotemporal variability of snow water equivalent (SWE) and soil moisture during snowmelt was undertaken at a subalpine forested setting with north, south, and flat aspects as a seasonally persistent snowpack melts. We investigated if evidence of preferential flow paths in snow can be observed and the effect on soil moisture through measurements of snow water equivalent and near-surface soil moisture, observing how SWE and near-surface soil moisture vary on hillslopes relative to the toes of hillslopes and flat areas. We then compared snowmelt infiltration beyond the near-surface soil between flat and sloping terrain during the entire snowmelt season using soil moisture sensor profiles. This study was conducted during varying snowmelt seasons representing above-normal, relatively normal, and below-normal snow seasons in northern Colorado. Evidence is presented of preferential meltwater flow paths at the snow-soil interface on the north-facing slope causing increases in SWE downslope and less infiltration into the soil at 20 cm depth; less association is observed in the near-surface soil moisture (top 7 cm). We present a conceptualization of the meltwater flow paths that develop based on slope aspect and soil properties. The resulting flow paths are shown to divert at least 4 % of snowmelt laterally, accumulating along the length of the slope, to increase the snow water equivalent by as much as 170 % at the base of a north-facing hillslope. Results from this study show that snow acts as an extension of the vadose zone during spring snowmelt and future hydrologic investigations will benefit from studying the snow and soil together.