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Sample records for lead 181

  1. 40 CFR 98.181 - Reporting threshold.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) MANDATORY GREENHOUSE GAS REPORTING Lead Production § 98.181 Reporting threshold. You must report GHG emissions under this subpart if your facility contains a lead production process and the facility meets...

  2. Lead

    MedlinePlus

    ... Lead Share Facebook Twitter Google+ Pinterest Contact Us Lead Poisoning is Preventable If your home was built before ... of the RRP rule. Read more . Learn about Lead Poisoning Prevention Week . Report Uncertified Contractors and Environmental Violations ...

  3. Lead

    MedlinePlus

    ... obvious symptoms, it frequently goes unrecognized. CDC’s Childhood Lead Poisoning Prevention Program is committed to the Healthy People ... Lead Levels Information for Parents Tips for preventing lead poisoning About Us Overview of CDC’s Childhood Lead Poisoning ...

  4. 21 CFR 181.24 - Antioxidants.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Antioxidants. 181.24 Section 181.24 Food and Drugs... INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.24 Antioxidants. Substances classified as antioxidants, when migrating from food-packaging material (limit of addition to food, 0.005 percent)...

  5. 12 CFR 303.181 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 4 2011-01-01 2011-01-01 false Definitions. 303.181 Section 303.181 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION PROCEDURE AND RULES OF PRACTICE FILING PROCEDURES International Banking § 303.181 Definitions. For the purposes of this subpart, the following...

  6. 5 CFR 550.181 - Coverage.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 5 Administrative Personnel 1 2012-01-01 2012-01-01 false Coverage. 550.181 Section 550.181 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY ADMINISTRATION (GENERAL) Premium Pay Law Enforcement Availability Pay § 550.181 Coverage. (a) Each employee meeting the...

  7. 5 CFR 550.181 - Coverage.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 5 Administrative Personnel 1 2014-01-01 2014-01-01 false Coverage. 550.181 Section 550.181 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY ADMINISTRATION (GENERAL) Premium Pay Law Enforcement Availability Pay § 550.181 Coverage. (a) Each employee meeting the...

  8. 5 CFR 550.181 - Coverage.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 5 Administrative Personnel 1 2013-01-01 2013-01-01 false Coverage. 550.181 Section 550.181 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY ADMINISTRATION (GENERAL) Premium Pay Law Enforcement Availability Pay § 550.181 Coverage. (a) Each employee meeting the...

  9. 46 CFR 181.600 - Fire axe.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Fire axe. 181.600 Section 181.600 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Additional Equipment § 181.600 Fire axe. A vessel of more than 19.8 meters (65 feet) in...

  10. 46 CFR 181.600 - Fire axe.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Fire axe. 181.600 Section 181.600 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Additional Equipment § 181.600 Fire axe. A vessel of more than 19.8 meters (65 feet) in...

  11. 46 CFR 181.610 - Fire bucket.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Fire bucket. 181.610 Section 181.610 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Additional Equipment § 181.610 Fire bucket. A vessel not required to have a power...

  12. 46 CFR 181.300 - Fire pumps.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Fire pumps. 181.300 Section 181.300 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Fire Main System § 181.300 Fire pumps. (a) A self priming, power driven fire pump must...

  13. 46 CFR 181.600 - Fire axe.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Fire axe. 181.600 Section 181.600 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Additional Equipment § 181.600 Fire axe. A vessel of more than 19.8 meters (65 feet) in...

  14. 46 CFR 181.300 - Fire pumps.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Fire pumps. 181.300 Section 181.300 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Fire Main System § 181.300 Fire pumps. (a) A self priming, power driven fire pump must...

  15. 46 CFR 181.610 - Fire bucket.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Fire bucket. 181.610 Section 181.610 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Additional Equipment § 181.610 Fire bucket. A vessel not required to have a power...

  16. 46 CFR 181.600 - Fire axe.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Fire axe. 181.600 Section 181.600 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Additional Equipment § 181.600 Fire axe. A vessel of more than 19.8 meters (65 feet) in...

  17. 46 CFR 181.600 - Fire axe.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Fire axe. 181.600 Section 181.600 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Additional Equipment § 181.600 Fire axe. A vessel of more than 19.8 meters (65 feet) in...

  18. 46 CFR 181.610 - Fire bucket.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Fire bucket. 181.610 Section 181.610 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Additional Equipment § 181.610 Fire bucket. A vessel not required to have a power...

  19. 46 CFR 181.610 - Fire bucket.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Fire bucket. 181.610 Section 181.610 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Additional Equipment § 181.610 Fire bucket. A vessel not required to have a power...

  20. 46 CFR 181.300 - Fire pumps.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Fire pumps. 181.300 Section 181.300 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Fire Main System § 181.300 Fire pumps. (a) A self priming, power driven fire pump must...

  1. 46 CFR 181.610 - Fire bucket.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Fire bucket. 181.610 Section 181.610 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Additional Equipment § 181.610 Fire bucket. A vessel not required to have a power...

  2. 49 CFR 37.181 - Applicability dates.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 1 2010-10-01 2010-10-01 false Applicability dates. 37.181 Section 37.181 Transportation Office of the Secretary of Transportation TRANSPORTATION SERVICES FOR INDIVIDUALS WITH DISABILITIES (ADA) Over-the-Road Buses (OTRBs) § 37.181 Applicability dates. This subpart applies to...

  3. 49 CFR 37.181 - Applicability dates.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 1 2011-10-01 2011-10-01 false Applicability dates. 37.181 Section 37.181 Transportation Office of the Secretary of Transportation TRANSPORTATION SERVICES FOR INDIVIDUALS WITH DISABILITIES (ADA) Over-the-Road Buses (OTRBs) § 37.181 Applicability dates. This subpart applies to...

  4. 21 CFR 181.28 - Release agents.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Release agents. 181.28 Section 181.28 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.28 Release agents....

  5. 25 CFR 700.181 - Eligibility.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Eligibility. 700.181 Section 700.181 Indians THE OFFICE OF NAVAJO AND HOPI INDIAN RELOCATION COMMISSION OPERATIONS AND RELOCATION PROCEDURES Replacement Housing Payments § 700.181 Eligibility. (a) Basic eligibility requirements. A certified eligible head...

  6. 25 CFR 700.181 - Eligibility.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false Eligibility. 700.181 Section 700.181 Indians THE OFFICE OF NAVAJO AND HOPI INDIAN RELOCATION COMMISSION OPERATIONS AND RELOCATION PROCEDURES Replacement Housing Payments § 700.181 Eligibility. (a) Basic eligibility requirements. A certified eligible head...

  7. 25 CFR 700.181 - Eligibility.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 2 2012-04-01 2012-04-01 false Eligibility. 700.181 Section 700.181 Indians THE OFFICE OF NAVAJO AND HOPI INDIAN RELOCATION COMMISSION OPERATIONS AND RELOCATION PROCEDURES Replacement Housing Payments § 700.181 Eligibility. (a) Basic eligibility requirements. A certified eligible head...

  8. 25 CFR 700.181 - Eligibility.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 2 2011-04-01 2011-04-01 false Eligibility. 700.181 Section 700.181 Indians THE OFFICE OF NAVAJO AND HOPI INDIAN RELOCATION COMMISSION OPERATIONS AND RELOCATION PROCEDURES Replacement Housing Payments § 700.181 Eligibility. (a) Basic eligibility requirements. A certified eligible head...

  9. 46 CFR 201.181 - General matters.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 8 2012-10-01 2012-10-01 false General matters. 201.181 Section 201.181 Shipping... PROCEDURE Judicial Standards of Practice (Rule 19) § 201.181 General matters. (a) In general, the functions... after notice and opportunity for hearing, or in the case of other matters from the time of notice by...

  10. 46 CFR 201.181 - General matters.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 8 2013-10-01 2013-10-01 false General matters. 201.181 Section 201.181 Shipping... PROCEDURE Judicial Standards of Practice (Rule 19) § 201.181 General matters. (a) In general, the functions... after notice and opportunity for hearing, or in the case of other matters from the time of notice by...

  11. 46 CFR 201.181 - General matters.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false General matters. 201.181 Section 201.181 Shipping... PROCEDURE Judicial Standards of Practice (Rule 19) § 201.181 General matters. (a) In general, the functions... after notice and opportunity for hearing, or in the case of other matters from the time of notice by...

  12. 46 CFR 201.181 - General matters.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 8 2011-10-01 2011-10-01 false General matters. 201.181 Section 201.181 Shipping... PROCEDURE Judicial Standards of Practice (Rule 19) § 201.181 General matters. (a) In general, the functions... after notice and opportunity for hearing, or in the case of other matters from the time of notice by...

  13. 46 CFR 201.181 - General matters.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 8 2014-10-01 2014-10-01 false General matters. 201.181 Section 201.181 Shipping... PROCEDURE Judicial Standards of Practice (Rule 19) § 201.181 General matters. (a) In general, the functions... after notice and opportunity for hearing, or in the case of other matters from the time of notice by...

  14. 5 CFR 550.181 - Coverage.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 5 Administrative Personnel 1 2011-01-01 2011-01-01 false Coverage. 550.181 Section 550.181 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY ADMINISTRATION (GENERAL) Premium Pay Law Enforcement Availability Pay § 550.181 Coverage. (a) Each employee meeting the...

  15. 5 CFR 550.181 - Coverage.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Coverage. 550.181 Section 550.181 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY ADMINISTRATION (GENERAL) Premium Pay Law Enforcement Availability Pay § 550.181 Coverage. (a) Each employee meeting the...

  16. 10 CFR 60.181 - Violations.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 2 2014-01-01 2014-01-01 false Violations. 60.181 Section 60.181 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) DISPOSAL OF HIGH-LEVEL RADIOACTIVE WASTES IN GEOLOGIC REPOSITORIES Violations § 60.181 Violations. (a) The Commission may obtain an injunction or other court order to prevent...

  17. 10 CFR 60.181 - Violations.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 2 2011-01-01 2011-01-01 false Violations. 60.181 Section 60.181 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) DISPOSAL OF HIGH-LEVEL RADIOACTIVE WASTES IN GEOLOGIC REPOSITORIES Violations § 60.181 Violations. (a) The Commission may obtain an injunction or other court order to prevent...

  18. 10 CFR 60.181 - Violations.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 2 2012-01-01 2012-01-01 false Violations. 60.181 Section 60.181 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) DISPOSAL OF HIGH-LEVEL RADIOACTIVE WASTES IN GEOLOGIC REPOSITORIES Violations § 60.181 Violations. (a) The Commission may obtain an injunction or other court order to prevent...

  19. 10 CFR 60.181 - Violations.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 2 2013-01-01 2013-01-01 false Violations. 60.181 Section 60.181 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) DISPOSAL OF HIGH-LEVEL RADIOACTIVE WASTES IN GEOLOGIC REPOSITORIES Violations § 60.181 Violations. (a) The Commission may obtain an injunction or other court order to prevent...

  20. 10 CFR 60.181 - Violations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Violations. 60.181 Section 60.181 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) DISPOSAL OF HIGH-LEVEL RADIOACTIVE WASTES IN GEOLOGIC REPOSITORIES Violations § 60.181 Violations. (a) The Commission may obtain an injunction or other court order to prevent...

  1. 14 CFR 25.181 - Dynamic stability.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Dynamic stability. 25.181 Section 25.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Flight Stability § 25.181 Dynamic stability. (a) Any short...

  2. 14 CFR 25.181 - Dynamic stability.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Dynamic stability. 25.181 Section 25.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Flight Stability § 25.181 Dynamic stability. (a) Any short...

  3. 14 CFR 25.181 - Dynamic stability.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Dynamic stability. 25.181 Section 25.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Flight Stability § 25.181 Dynamic stability. (a) Any short...

  4. 14 CFR 23.181 - Dynamic stability.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Dynamic stability. 23.181 Section 23.181... STANDARDS: NORMAL, UTILITY, ACROBATIC, AND COMMUTER CATEGORY AIRPLANES Flight Stability § 23.181 Dynamic stability. (a) Any short period oscillation not including combined lateral-directional...

  5. 14 CFR 23.181 - Dynamic stability.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Dynamic stability. 23.181 Section 23.181... STANDARDS: NORMAL, UTILITY, ACROBATIC, AND COMMUTER CATEGORY AIRPLANES Flight Stability § 23.181 Dynamic stability. Link to an amendment published at 76 FR 75755, December 2, 2011. (a) Any short period...

  6. 14 CFR 25.181 - Dynamic stability.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Dynamic stability. 25.181 Section 25.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Flight Stability § 25.181 Dynamic stability. (a) Any short...

  7. 14 CFR 23.181 - Dynamic stability.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Dynamic stability. 23.181 Section 23.181... STANDARDS: NORMAL, UTILITY, ACROBATIC, AND COMMUTER CATEGORY AIRPLANES Flight Stability § 23.181 Dynamic stability. (a) Any short period oscillation not including combined lateral-directional...

  8. 14 CFR 25.181 - Dynamic stability.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Dynamic stability. 25.181 Section 25.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY AIRPLANES Flight Stability § 25.181 Dynamic stability. (a) Any short...

  9. 30 CFR 817.181 - Support facilities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Support facilities. 817.181 Section 817.181... ACTIVITIES § 817.181 Support facilities. (a) Support facilities shall be operated in accordance with a permit issued for the mine or coal preparation plant to which it is incident or from which its operation...

  10. 30 CFR 816.181 - Support facilities.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 3 2010-07-01 2010-07-01 false Support facilities. 816.181 Section 816.181... § 816.181 Support facilities. (a) Support facilities shall be operated in accordance with a permit issued for the mine or coal preparation operation to which it is incident or from which its...

  11. 21 CFR 181.23 - Antimycotics.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Antimycotics. 181.23 Section 181.23 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.23 Antimycotics. Substances classified...

  12. 21 CFR 181.5 - Prior sanctions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... FOOD INGREDIENTS General Provisions § 181.5 Prior sanctions. (a) A prior sanction shall exist only for... food additive or GRAS regulation promulgated after a general evaluation of use of an ingredient... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Prior sanctions. 181.5 Section 181.5 Food...

  13. 21 CFR 181.5 - Prior sanctions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS General Provisions § 181.5 Prior sanctions. (a) A... use of the ingredient, in order to prevent the adulteration of food in violation of section 402 of the... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Prior sanctions. 181.5 Section 181.5 Food...

  14. 21 CFR 181.5 - Prior sanctions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS General Provisions § 181.5 Prior sanctions. (a) A... use of the ingredient, in order to prevent the adulteration of food in violation of section 402 of the... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Prior sanctions. 181.5 Section 181.5 Food and...

  15. 21 CFR 181.1 - General.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... INGREDIENTS General Provisions § 181.1 General. (a) An ingredient whose use in food or food packaging is... information that shows that use of a prior-sanctioned food ingredient may be injurious to health, and thus in... 21 Food and Drugs 3 2014-04-01 2014-04-01 false General. 181.1 Section 181.1 Food and Drugs...

  16. 21 CFR 181.27 - Plasticizers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Plasticizers. 181.27 Section 181.27 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.27 Plasticizers. Substances classified...

  17. 21 CFR 181.5 - Prior sanctions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS General Provisions § 181.5 Prior sanctions. (a) A... use of the ingredient, in order to prevent the adulteration of food in violation of section 402 of the... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Prior sanctions. 181.5 Section 181.5 Food...

  18. 21 CFR 181.5 - Prior sanctions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS General Provisions § 181.5 Prior sanctions. (a) A... use of the ingredient, in order to prevent the adulteration of food in violation of section 402 of the... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Prior sanctions. 181.5 Section 181.5 Food...

  19. 12 CFR 303.181 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Definitions. 303.181 Section 303.181 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION PROCEDURE AND RULES OF PRACTICE FILING PROCEDURES International Banking § 303.181 Definitions. For the purposes of this subpart, the following...

  20. 21 CFR 181.24 - Antioxidants.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Antioxidants. 181.24 Section 181.24 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN... Antioxidants. Substances classified as antioxidants, when migrating from food-packaging material (limit...

  1. 21 CFR 181.24 - Antioxidants.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Antioxidants. 181.24 Section 181.24 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN... Antioxidants. Substances classified as antioxidants, when migrating from food-packaging material (limit...

  2. 21 CFR 181.24 - Antioxidants.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Antioxidants. 181.24 Section 181.24 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN... Antioxidants. Substances classified as antioxidants, when migrating from food-packaging material (limit...

  3. 21 CFR 181.28 - Release agents.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Release agents. 181.28 Section 181.28 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  4. 21 CFR 181.28 - Release agents.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Release agents. 181.28 Section 181.28 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  5. 21 CFR 181.28 - Release agents.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Release agents. 181.28 Section 181.28 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  6. 21 CFR 181.28 - Release agents.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Release agents. 181.28 Section 181.28 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  7. 27 CFR 479.181 - Penalties.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2012-04-01 2010-04-01 true Penalties. 479.181 Section 479.181 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND CERTAIN OTHER...

  8. 27 CFR 479.181 - Penalties.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2010-04-01 2010-04-01 false Penalties. 479.181 Section 479.181 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND CERTAIN OTHER...

  9. 27 CFR 479.181 - Penalties.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2014-04-01 2014-04-01 false Penalties. 479.181 Section 479.181 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND CERTAIN OTHER...

  10. 27 CFR 479.181 - Penalties.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2011-04-01 2010-04-01 true Penalties. 479.181 Section 479.181 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND CERTAIN OTHER...

  11. 27 CFR 479.181 - Penalties.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2013-04-01 2013-04-01 false Penalties. 479.181 Section 479.181 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE FIREARMS AND AMMUNITION MACHINE GUNS, DESTRUCTIVE DEVICES, AND CERTAIN OTHER...

  12. 21 CFR 181.24 - Antioxidants.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Antioxidants. 181.24 Section 181.24 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN... Antioxidants. Substances classified as antioxidants, when migrating from food-packaging material (limit...

  13. 10 CFR 26.181 - Purpose.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Purpose. 26.181 Section 26.181 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Determining Fitness-for-Duty Policy Violations and Determining Fitness... FFD policy and for making a determination of fitness....

  14. 21 CFR 181.23 - Antimycotics.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Antimycotics. 181.23 Section 181.23 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  15. 21 CFR 181.25 - Driers.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Driers. 181.25 Section 181.25 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  16. 21 CFR 181.27 - Plasticizers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Plasticizers. 181.27 Section 181.27 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  17. 21 CFR 181.23 - Antimycotics.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Antimycotics. 181.23 Section 181.23 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  18. 21 CFR 181.25 - Driers.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Driers. 181.25 Section 181.25 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  19. 21 CFR 181.25 - Driers.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Driers. 181.25 Section 181.25 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  20. 21 CFR 181.27 - Plasticizers.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Plasticizers. 181.27 Section 181.27 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  1. 21 CFR 181.27 - Plasticizers.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Plasticizers. 181.27 Section 181.27 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  2. 21 CFR 181.27 - Plasticizers.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Plasticizers. 181.27 Section 181.27 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  3. 21 CFR 181.23 - Antimycotics.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Antimycotics. 181.23 Section 181.23 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  4. 21 CFR 181.23 - Antimycotics.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Antimycotics. 181.23 Section 181.23 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  5. 21 CFR 181.25 - Driers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Driers. 181.25 Section 181.25 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  6. 10 CFR 26.181 - Purpose.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Purpose. 26.181 Section 26.181 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Determining Fitness-for-Duty Policy Violations and Determining Fitness... FFD policy and for making a determination of fitness....

  7. 10 CFR 26.181 - Purpose.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Purpose. 26.181 Section 26.181 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Determining Fitness-for-Duty Policy Violations and Determining Fitness... FFD policy and for making a determination of fitness....

  8. 10 CFR 26.181 - Purpose.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Purpose. 26.181 Section 26.181 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Determining Fitness-for-Duty Policy Violations and Determining Fitness... FFD policy and for making a determination of fitness....

  9. 10 CFR 26.181 - Purpose.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Purpose. 26.181 Section 26.181 Energy NUCLEAR REGULATORY COMMISSION FITNESS FOR DUTY PROGRAMS Determining Fitness-for-Duty Policy Violations and Determining Fitness... FFD policy and for making a determination of fitness....

  10. 40 CFR 408.181 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Specialized definitions. 408.181 Section 408.181 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) EFFLUENT GUIDELINES AND STANDARDS CANNED AND PRESERVED SEAFOOD PROCESSING POINT SOURCE CATEGORY West Coast...

  11. Munc18-1 is a molecular chaperone for α-synuclein, controlling its self-replicating aggregation.

    PubMed

    Chai, Ye Jin; Sierecki, Emma; Tomatis, Vanesa M; Gormal, Rachel S; Giles, Nichole; Morrow, Isabel C; Xia, Di; Götz, Jürgen; Parton, Robert G; Collins, Brett M; Gambin, Yann; Meunier, Frédéric A

    2016-09-12

    Munc18-1 is a key component of the exocytic machinery that controls neurotransmitter release. Munc18-1 heterozygous mutations cause developmental defects and epileptic phenotypes, including infantile epileptic encephalopathy (EIEE), suggestive of a gain of pathological function. Here, we used single-molecule analysis, gene-edited cells, and neurons to demonstrate that Munc18-1 EIEE-causing mutants form large polymers that coaggregate wild-type Munc18-1 in vitro and in cells. Surprisingly, Munc18-1 EIEE mutants also form Lewy body-like structures that contain α-synuclein (α-Syn). We reveal that Munc18-1 binds α-Syn, and its EIEE mutants coaggregate α-Syn. Likewise, removal of endogenous Munc18-1 increases the aggregative propensity of α-Syn(WT) and that of the Parkinson's disease-causing α-Syn(A30P) mutant, an effect rescued by Munc18-1(WT) expression, indicative of chaperone activity. Coexpression of the α-Syn(A30P) mutant with Munc18-1 reduced the number of α-Syn(A30P) aggregates. Munc18-1 mutations and haploinsufficiency may therefore trigger a pathogenic gain of function through both the corruption of native Munc18-1 and a perturbed chaperone activity for α-Syn leading to aggregation-induced neurodegeneration. PMID:27597756

  12. Munc18-1 is a molecular chaperone for α-synuclein, controlling its self-replicating aggregation.

    PubMed

    Chai, Ye Jin; Sierecki, Emma; Tomatis, Vanesa M; Gormal, Rachel S; Giles, Nichole; Morrow, Isabel C; Xia, Di; Götz, Jürgen; Parton, Robert G; Collins, Brett M; Gambin, Yann; Meunier, Frédéric A

    2016-09-12

    Munc18-1 is a key component of the exocytic machinery that controls neurotransmitter release. Munc18-1 heterozygous mutations cause developmental defects and epileptic phenotypes, including infantile epileptic encephalopathy (EIEE), suggestive of a gain of pathological function. Here, we used single-molecule analysis, gene-edited cells, and neurons to demonstrate that Munc18-1 EIEE-causing mutants form large polymers that coaggregate wild-type Munc18-1 in vitro and in cells. Surprisingly, Munc18-1 EIEE mutants also form Lewy body-like structures that contain α-synuclein (α-Syn). We reveal that Munc18-1 binds α-Syn, and its EIEE mutants coaggregate α-Syn. Likewise, removal of endogenous Munc18-1 increases the aggregative propensity of α-Syn(WT) and that of the Parkinson's disease-causing α-Syn(A30P) mutant, an effect rescued by Munc18-1(WT) expression, indicative of chaperone activity. Coexpression of the α-Syn(A30P) mutant with Munc18-1 reduced the number of α-Syn(A30P) aggregates. Munc18-1 mutations and haploinsufficiency may therefore trigger a pathogenic gain of function through both the corruption of native Munc18-1 and a perturbed chaperone activity for α-Syn leading to aggregation-induced neurodegeneration.

  13. 27 CFR 18.1 - Scope.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... TREASURY LIQUORS PRODUCTION OF VOLATILE FRUIT-FLAVOR CONCENTRATE Scope § 18.1 Scope. The regulations in... for the manufacture of volatile fruit-flavor concentrate (essence). The regulations in this part...

  14. 27 CFR 18.1 - Scope.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... TREASURY LIQUORS PRODUCTION OF VOLATILE FRUIT-FLAVOR CONCENTRATE Scope § 18.1 Scope. The regulations in... for the manufacture of volatile fruit-flavor concentrate (essence). The regulations in this part...

  15. 15 CFR 922.181 - Boundary.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... MARINE SANCTUARY PROGRAM REGULATIONS Hawaiian Islands Humpback Whale National Marine Sanctuary § 922.181... Humpback Whale National Marine Sanctuary consists of the submerged lands and waters off the coast of...

  16. 15 CFR 922.181 - Boundary.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... MARINE SANCTUARY PROGRAM REGULATIONS Hawaiian Islands Humpback Whale National Marine Sanctuary § 922.181... Humpback Whale National Marine Sanctuary consists of the submerged lands and waters off the coast of...

  17. 15 CFR 922.181 - Boundary.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... MARINE SANCTUARY PROGRAM REGULATIONS Hawaiian Islands Humpback Whale National Marine Sanctuary § 922.181... Humpback Whale National Marine Sanctuary consists of the submerged lands and waters off the coast of...

  18. 15 CFR 922.181 - Boundary.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... MARINE SANCTUARY PROGRAM REGULATIONS Hawaiian Islands Humpback Whale National Marine Sanctuary § 922.181... Humpback Whale National Marine Sanctuary consists of the submerged lands and waters off the coast of...

  19. 15 CFR 922.181 - Boundary.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... MARINE SANCTUARY PROGRAM REGULATIONS Hawaiian Islands Humpback Whale National Marine Sanctuary § 922.181... Humpback Whale National Marine Sanctuary consists of the submerged lands and waters off the coast of...

  20. 40 CFR 18.1 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL ENVIRONMENTAL PROTECTION RESEARCH FELLOWSHIPS AND SPECIAL RESEARCH CONSULTANTS FOR ENVIRONMENTAL PROTECTION § 18.1 Definitions. As used in this... delegated. An Environmental Protection Research Fellowship is one which requires the performance of...

  1. 40 CFR 18.1 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL ENVIRONMENTAL PROTECTION RESEARCH FELLOWSHIPS AND SPECIAL RESEARCH CONSULTANTS FOR ENVIRONMENTAL PROTECTION § 18.1 Definitions. As used in this... delegated. An Environmental Protection Research Fellowship is one which requires the performance of...

  2. 40 CFR 18.1 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL ENVIRONMENTAL PROTECTION RESEARCH FELLOWSHIPS AND SPECIAL RESEARCH CONSULTANTS FOR ENVIRONMENTAL PROTECTION § 18.1 Definitions. As used in this... delegated. An Environmental Protection Research Fellowship is one which requires the performance of...

  3. 40 CFR 18.1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL ENVIRONMENTAL PROTECTION RESEARCH FELLOWSHIPS AND SPECIAL RESEARCH CONSULTANTS FOR ENVIRONMENTAL PROTECTION § 18.1 Definitions. As used in this... delegated. An Environmental Protection Research Fellowship is one which requires the performance of...

  4. 40 CFR 18.1 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL ENVIRONMENTAL PROTECTION RESEARCH FELLOWSHIPS AND SPECIAL RESEARCH CONSULTANTS FOR ENVIRONMENTAL PROTECTION § 18.1 Definitions. As used in this... delegated. An Environmental Protection Research Fellowship is one which requires the performance of...

  5. Alpha decay of {sup 181}Pb

    SciTech Connect

    Davids, C.N.; Henderson, D.J.; Hermann, R.

    1995-08-01

    The {alpha}-decay energy of {sup 181}Pb was measured as 7211(10) keV and 7044(15). In the first study the isotope was produced in {sup 90}Zr bombardments of {sup 94}Mo and, after traversing a velocity filter, implanted in a position-sensitive Si detector; no half life for {sup 181}Pb was reported. In the second study the isotope was produced in {sup 40}Ca bombardments of {sup 144}Sm and transported to a position in front of a Si(Au) surface barrier detector with a fast He-gas-jet capillary system; an estimate of 50 ms was determined for the {sup 181}Pb half life. Recently we investigated {sup 181}Pb {alpha} decay at ATLAS as part of a survey experiment in which a l-pnA beam of 400-MeV {sup 92}Mo was used to irradiate targets of {sup 89}Y, {sup 90,92,94}Zr, and {sup 92}Mo to examine yields for one- and two-nucleon evaporation products from symmetric cold-fusion reactions. Recoiling nuclei of interest were passed through the Fragment Mass Analyzer and implanted in a double-sided silicon strip detector for {alpha}-particle assay. With the {sup 90}Zr target we observed a group at 7065(20) keV which was correlated with A = 181 recoils and had a half life of 45(20) ms. Our new results for {sup 181}Pb therefore agreed with those of the second study. There was no indication in the {sup 90}Zr + {sup 92}Mo data of the 7211(10)-keV {alpha} particles seen by Keller et al. The interested reader is referred to the 1993 atomic mass evaluation wherein the input {alpha}-decay energies and resultant masses of the light Pb isotopes (including {sup 181}Pb) are discussed.

  6. 46 CFR 181.500 - Required number, type, and location.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... section. Table 181.500(a) Space protected Minimum No. required Type extinguisher permitted CG class Medium... 46 Shipping 7 2010-10-01 2010-10-01 false Required number, type, and location. 181.500 Section 181... 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Portable Fire Extinguishers § 181.500 Required number,...

  7. 46 CFR 181.500 - Required number, type, and location.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... section. Table 181.500(a) Space protected Minimum No. required Type extinguisher permitted CG class Medium... 46 Shipping 7 2013-10-01 2013-10-01 false Required number, type, and location. 181.500 Section 181... 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Portable Fire Extinguishers § 181.500 Required number,...

  8. 46 CFR 181.500 - Required number, type, and location.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... section. Table 181.500(a) Space protected Minimum No. required Type extinguisher permitted CG class Medium... 46 Shipping 7 2014-10-01 2014-10-01 false Required number, type, and location. 181.500 Section 181... 100 GROSS TONS) FIRE PROTECTION EQUIPMENT Portable Fire Extinguishers § 181.500 Required number,...

  9. 19 CFR 181.131 - Rules of origin.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... in effect prior to October 1, 1995 (see 19 CFR chapter I, 1994 edition, appendix to part 181... 19 Customs Duties 2 2013-04-01 2013-04-01 false Rules of origin. 181.131 Section 181.131 Customs... (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Rules of Origin § 181.131 Rules of origin. (a)...

  10. 19 CFR 181.131 - Rules of origin.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... in effect prior to October 1, 1995 (see 19 CFR chapter I, 1994 edition, appendix to part 181... 19 Customs Duties 2 2010-04-01 2010-04-01 false Rules of origin. 181.131 Section 181.131 Customs... (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Rules of Origin § 181.131 Rules of origin. (a)...

  11. 19 CFR 181.131 - Rules of origin.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... in effect prior to October 1, 1995 (see 19 CFR chapter I, 1994 edition, appendix to part 181... 19 Customs Duties 2 2012-04-01 2012-04-01 false Rules of origin. 181.131 Section 181.131 Customs... (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Rules of Origin § 181.131 Rules of origin. (a)...

  12. 19 CFR 181.131 - Rules of origin.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... in effect prior to October 1, 1995 (see 19 CFR chapter I, 1994 edition, appendix to part 181... 19 Customs Duties 2 2014-04-01 2014-04-01 false Rules of origin. 181.131 Section 181.131 Customs... (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Rules of Origin § 181.131 Rules of origin. (a)...

  13. 19 CFR 181.131 - Rules of origin.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... in effect prior to October 1, 1995 (see 19 CFR chapter I, 1994 edition, appendix to part 181... 19 Customs Duties 2 2011-04-01 2011-04-01 false Rules of origin. 181.131 Section 181.131 Customs... (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Rules of Origin § 181.131 Rules of origin. (a)...

  14. 19 CFR 181.122 - Disclosure to government authorities.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Disclosure to government authorities. 181.122 Section 181.122 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY... Information § 181.122 Disclosure to government authorities. Nothing in § 181.121 of this part shall...

  15. 19 CFR 181.101 - Publication of decisions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Advance Ruling Procedures § 181.101 Publication of... 19 Customs Duties 2 2010-04-01 2010-04-01 false Publication of decisions. 181.101 Section 181.101.... Disclosure is governed by 31 CFR part 1, part 103 of this chapter, and § 181.99(a)(3) of this part....

  16. 19 CFR 181.101 - Publication of decisions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Advance Ruling Procedures § 181.101 Publication of... 19 Customs Duties 2 2012-04-01 2012-04-01 false Publication of decisions. 181.101 Section 181.101.... Disclosure is governed by 31 CFR part 1, part 103 of this chapter, and § 181.99(a)(3) of this part....

  17. 19 CFR 181.101 - Publication of decisions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Advance Ruling Procedures § 181.101 Publication of... 19 Customs Duties 2 2011-04-01 2011-04-01 false Publication of decisions. 181.101 Section 181.101.... Disclosure is governed by 31 CFR part 1, part 103 of this chapter, and § 181.99(a)(3) of this part....

  18. 19 CFR 181.101 - Publication of decisions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Advance Ruling Procedures § 181.101 Publication of... 19 Customs Duties 2 2013-04-01 2013-04-01 false Publication of decisions. 181.101 Section 181.101.... Disclosure is governed by 31 CFR part 1, part 103 of this chapter, and § 181.99(a)(3) of this part....

  19. 49 CFR 572.181 - General description.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) ANTHROPOMORPHIC TEST DEVICES 2re Side Impact Crash Test Dummy, 50th Percentile Adult Male § 572.181 General description. (a) The ES-2re Side Impact Crash Test...,” containing 9 pages, incorporated by reference, see § 572.180, (3) A listing of available...

  20. 49 CFR 572.181 - General description.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) ANTHROPOMORPHIC TEST DEVICES ES-2re Side Impact Crash Test Dummy, 50th Percentile Adult Male § 572.181 General description. (a) The ES-2re Side Impact Crash...,” containing 9 pages, incorporated by reference, see § 572.180, (3) A listing of available...

  1. 49 CFR 572.181 - General description.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) ANTHROPOMORPHIC TEST DEVICES 2re Side Impact Crash Test Dummy, 50th Percentile Adult Male § 572.181 General description. (a) The ES-2re Side Impact Crash Test... transducers-crash test sensors for the ES-2re Crash Test Dummy is shown in drawing 175-0000 sheet 4 of...

  2. 49 CFR 572.181 - General description.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) ANTHROPOMORPHIC TEST DEVICES ES-2re Side Impact Crash Test Dummy, 50th Percentile Adult Male § 572.181 General description. (a) The ES-2re Side Impact Crash...,” containing 9 pages, incorporated by reference, see § 572.180, (3) A listing of available...

  3. 40 CFR 417.181 - Specialized definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Manufacture of Drum Dried Detergents Subcategory § 417.181 Specialized definitions. For the purpose of this subpart: (a) Except as provided below, the general definitions, abbreviations and methods of analysis set forth in 40 CFR part...

  4. 40 CFR 417.181 - Specialized definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Manufacture of Drum Dried Detergents Subcategory § 417.181 Specialized definitions. For the purpose of this subpart: (a) Except as provided below, the general definitions, abbreviations and methods of analysis set forth in 40 CFR part...

  5. 40 CFR 417.181 - Specialized definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Manufacture of Drum Dried Detergents Subcategory § 417.181 Specialized definitions. For the purpose of this subpart: (a) Except as provided below, the general definitions, abbreviations and methods of analysis set forth in 40 CFR part...

  6. 40 CFR 417.181 - Specialized definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Manufacture of Drum Dried Detergents Subcategory § 417.181 Specialized definitions. For the purpose of this subpart: (a) Except as provided below, the general definitions, abbreviations and methods of analysis set forth in 40 CFR part...

  7. 40 CFR 417.181 - Specialized definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... AND STANDARDS SOAP AND DETERGENT MANUFACTURING POINT SOURCE CATEGORY Manufacture of Drum Dried Detergents Subcategory § 417.181 Specialized definitions. For the purpose of this subpart: (a) Except as provided below, the general definitions, abbreviations and methods of analysis set forth in 40 CFR part...

  8. 22 CFR 181.3 - Determinations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.3 Determinations. (a) Whether any undertaking, document, or set of documents constitutes or would constitute an international agreement within the meaning of the Act or of 1 U.S.C....

  9. 46 CFR 181.400 - Where required.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... other fuel having a flash point of 43.3 °C (110 °F) or lower. (d) All griddles, broilers, and deep fat fryers must be fitted with a grease extraction hood in compliance with § 181.425. (e) Each...

  10. 10 CFR 501.181 - Sanctions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ..., Sanctions and Judicial Actions § 501.181 Sanctions. (a) General. (1) A violation of any provision of the Act... sanctions provided in subtitle C of title VII of FUA. (2) Each day that any provision of the Act (other than... who willfully violates any provision of the Act (other than section 402), or any rule or...

  11. 10 CFR 501.181 - Sanctions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ..., Sanctions and Judicial Actions § 501.181 Sanctions. (a) General. (1) A violation of any provision of the Act... sanctions provided in subtitle C of title VII of FUA. (2) Each day that any provision of the Act (other than... who willfully violates any provision of the Act (other than section 402), or any rule or...

  12. 40 CFR 61.181 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Inorganic Arsenic Emissions From Arsenic Trioxide and Metallic Arsenic Production Facilities § 61.181 Definitions. All terms used... section as follows: Arsenic kitchen means a baffled brick chamber where inorganic arsenic vapors...

  13. 40 CFR 61.181 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Inorganic Arsenic Emissions From Arsenic Trioxide and Metallic Arsenic Production Facilities § 61.181 Definitions. All terms used... section as follows: Arsenic kitchen means a baffled brick chamber where inorganic arsenic vapors...

  14. 40 CFR 61.181 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Inorganic Arsenic Emissions From Arsenic Trioxide and Metallic Arsenic Production Facilities § 61.181 Definitions. All terms used... section as follows: Arsenic kitchen means a baffled brick chamber where inorganic arsenic vapors...

  15. 40 CFR 61.181 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Inorganic Arsenic Emissions From Arsenic Trioxide and Metallic Arsenic Production Facilities § 61.181 Definitions. All terms used... section as follows: Arsenic kitchen means a baffled brick chamber where inorganic arsenic vapors...

  16. 40 CFR 61.181 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... EMISSION STANDARDS FOR HAZARDOUS AIR POLLUTANTS National Emission Standard for Inorganic Arsenic Emissions From Arsenic Trioxide and Metallic Arsenic Production Facilities § 61.181 Definitions. All terms used... section as follows: Arsenic kitchen means a baffled brick chamber where inorganic arsenic vapors...

  17. 46 CFR 181.400 - Where required.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... following spaces must be equipped with a fixed gas fire extinguishing system, in compliance with § 181.410... allowed by paragraph (b) of this section: (1) A space containing propulsion machinery; (2) A space containing an internal combustion engine of more than 37.3 kW (50 hp); (3) A space containing an oil...

  18. 46 CFR 181.400 - Where required.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... following spaces must be equipped with a fixed gas fire extinguishing system, in compliance with § 181.410... allowed by paragraph (b) of this section: (1) A space containing propulsion machinery; (2) A space containing an internal combustion engine of more than 37.3 kW (50 hp); (3) A space containing an oil...

  19. 46 CFR 181.400 - Where required.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... following spaces must be equipped with a fixed gas fire extinguishing system, in compliance with § 181.410... allowed by paragraph (b) of this section: (1) A space containing propulsion machinery; (2) A space containing an internal combustion engine of more than 37.3 kW (50 hp); (3) A space containing an oil...

  20. 46 CFR 181.400 - Where required.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... following spaces must be equipped with a fixed gas fire extinguishing system, in compliance with § 181.410... allowed by paragraph (b) of this section: (1) A space containing propulsion machinery; (2) A space containing an internal combustion engine of more than 37.3 kW (50 hp); (3) A space containing an oil...

  1. 21 CFR 181.1 - General.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS General Provisions § 181.1 General. (a) An ingredient whose use in food or food packaging is subject to a prior sanction or approval within the meaning...-sanctioned food ingredient may be injurious to health, and thus in violation of section 402 of the Act,...

  2. 21 CFR 181.1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS General Provisions § 181.1 General. (a) An ingredient whose use in food or food packaging is subject to a prior sanction or approval within the meaning...-sanctioned food ingredient may be injurious to health, and thus in violation of section 402 of the Act,...

  3. 21 CFR 181.1 - General.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS General Provisions § 181.1 General. (a) An ingredient whose use in food or food packaging is subject to a prior sanction or approval within the meaning...-sanctioned food ingredient may be injurious to health, and thus in violation of section 402 of the Act,...

  4. 21 CFR 181.1 - General.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS General Provisions § 181.1 General. (a) An ingredient whose use in food or food packaging is subject to a prior sanction or approval within the meaning...-sanctioned food ingredient may be injurious to health, and thus in violation of section 402 of the Act,...

  5. 14 CFR 23.181 - Dynamic stability.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... STANDARDS: NORMAL, UTILITY, ACROBATIC, AND COMMUTER CATEGORY AIRPLANES Flight Stability § 23.181 Dynamic... to meet the flight characteristic requirements of this part, the primary control requirements of... suddenly released, the response of the airplane must not exhibit any dangerous characteristics nor...

  6. 22 CFR 181.3 - Determinations.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.3 Determinations. (a) Whether any undertaking, document, or set of documents constitutes or would constitute an international agreement within the meaning of the Act or of 1 U.S.C....

  7. 26 CFR 1.181-4T - Special rules (temporary).

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... to § 1.181-2T(d). (2) Principal photography not commencing prior to January 1, 2009. If a taxpayer claims a deduction under section 181 with respect to a production for which principal photography...

  8. 26 CFR 1.181-4T - Special rules (temporary).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... to § 1.181-2T(d). (2) Principal photography not commencing prior to January 1, 2009. If a taxpayer claims a deduction under section 181 with respect to a production for which principal photography...

  9. 14 CFR 91.181 - Course to be flown.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 2 2010-01-01 2010-01-01 false Course to be flown. 91.181 Section 91.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... § 91.181 Course to be flown. Unless otherwise authorized by ATC, no person may operate an...

  10. 14 CFR 91.181 - Course to be flown.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 2 2011-01-01 2011-01-01 false Course to be flown. 91.181 Section 91.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... § 91.181 Course to be flown. Unless otherwise authorized by ATC, no person may operate an...

  11. 27 CFR 555.181 - Reporting of plastic explosives.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... explosives. 555.181 Section 555.181 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE EXPLOSIVES COMMERCE IN EXPLOSIVES Marking of Plastic Explosives § 555.181 Reporting of plastic explosives. All persons, other than an agency of the United...

  12. 27 CFR 555.181 - Reporting of plastic explosives.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... explosives. 555.181 Section 555.181 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE EXPLOSIVES COMMERCE IN EXPLOSIVES Marking of Plastic Explosives § 555.181 Reporting of plastic explosives. All persons, other than an agency of the United...

  13. 27 CFR 555.181 - Reporting of plastic explosives.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... explosives. 555.181 Section 555.181 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE EXPLOSIVES COMMERCE IN EXPLOSIVES Marking of Plastic Explosives § 555.181 Reporting of plastic explosives. All persons, other than an agency of the United...

  14. 27 CFR 555.181 - Reporting of plastic explosives.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... explosives. 555.181 Section 555.181 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE EXPLOSIVES COMMERCE IN EXPLOSIVES Marking of Plastic Explosives § 555.181 Reporting of plastic explosives. All persons, other than an agency of the United...

  15. 27 CFR 555.181 - Reporting of plastic explosives.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... explosives. 555.181 Section 555.181 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE EXPLOSIVES COMMERCE IN EXPLOSIVES Marking of Plastic Explosives § 555.181 Reporting of plastic explosives. All persons, other than an agency of the United...

  16. 21 CFR 145.181 - Artificially sweetened canned pineapple.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Artificially sweetened canned pineapple. 145.181 Section 145.181 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... § 145.181 Artificially sweetened canned pineapple. (a) Artificially sweetened canned pineapple is...

  17. 21 CFR 145.181 - Artificially sweetened canned pineapple.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Artificially sweetened canned pineapple. 145.181 Section 145.181 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... § 145.181 Artificially sweetened canned pineapple. (a) Artificially sweetened canned pineapple is...

  18. 21 CFR 145.181 - Artificially sweetened canned pineapple.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Artificially sweetened canned pineapple. 145.181 Section 145.181 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... § 145.181 Artificially sweetened canned pineapple. (a) Artificially sweetened canned pineapple is...

  19. 21 CFR 145.181 - Artificially sweetened canned pineapple.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Artificially sweetened canned pineapple. 145.181 Section 145.181 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... § 145.181 Artificially sweetened canned pineapple. (a) Artificially sweetened canned pineapple is...

  20. 21 CFR 145.181 - Artificially sweetened canned pineapple.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Artificially sweetened canned pineapple. 145.181 Section 145.181 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... § 145.181 Artificially sweetened canned pineapple. (a) Artificially sweetened canned pineapple is...

  1. 22 CFR 181.6 - Documentation and certification.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Documentation and certification. 181.6 Section 181.6 Foreign Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.6 Documentation and certification. (a) Transmittals of...

  2. 19 CFR 122.181 - Definition of Customs security area.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Definition of Customs security area. 122.181 Section 122.181 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY AIR COMMERCE REGULATIONS Access to Customs Security Areas § 122.181 Definition...

  3. 19 CFR 181.114 - Customs response to request.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Customs response to request. 181.114 Section 181.114 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF... Decisions § 181.114 Customs response to request. (a) Time for response. The port director will issue...

  4. 22 CFR 181.6 - Documentation and certification.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Documentation and certification. 181.6 Section 181.6 Foreign Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.6 Documentation and certification. (a) Transmittals of concluded agreements to the Assistant Legal...

  5. 21 CFR 820.181 - Device master record.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Device master record. 820.181 Section 820.181 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES QUALITY SYSTEM REGULATION Records § 820.181 Device master record. Each manufacturer shall...

  6. 19 CFR 181.121 - Maintenance of confidentiality.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Maintenance of confidentiality. 181.121 Section 181.121 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT... Information § 181.121 Maintenance of confidentiality. The port director or other Customs officer who...

  7. 46 CFR 181.310 - Fire main and hydrants.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Fire main and hydrants. 181.310 Section 181.310 Shipping...) FIRE PROTECTION EQUIPMENT Fire Main System § 181.310 Fire main and hydrants. (a) A vessel that has a power driven fire pump must have a sufficient number of fire hydrants to reach any part of the...

  8. 19 CFR 181.122 - Disclosure to government authorities.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Disclosure to government authorities. 181.122... Information § 181.122 Disclosure to government authorities. Nothing in § 181.121 of this part shall preclude the disclosure of confidential business information to governmental authorities in the United...

  9. 7 CFR 28.181 - Review of cotton classification.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 2 2013-01-01 2013-01-01 false Review of cotton classification. 28.181 Section 28.181... REGULATIONS COTTON CLASSING, TESTING, AND STANDARDS Classification for Foreign Growth Cotton § 28.181 Review of cotton classification. A review of any classification or comparison made pursuant to this...

  10. 7 CFR 28.181 - Review of cotton classification.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 2 2010-01-01 2010-01-01 false Review of cotton classification. 28.181 Section 28.181... REGULATIONS COTTON CLASSING, TESTING, AND STANDARDS Classification for Foreign Growth Cotton § 28.181 Review of cotton classification. A review of any classification or comparison made pursuant to this...

  11. 7 CFR 28.181 - Review of cotton classification.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 2 2011-01-01 2011-01-01 false Review of cotton classification. 28.181 Section 28.181... REGULATIONS COTTON CLASSING, TESTING, AND STANDARDS Classification for Foreign Growth Cotton § 28.181 Review of cotton classification. A review of any classification or comparison made pursuant to this...

  12. 7 CFR 28.181 - Review of cotton classification.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 2 2014-01-01 2014-01-01 false Review of cotton classification. 28.181 Section 28.181... REGULATIONS COTTON CLASSING, TESTING, AND STANDARDS Classification for Foreign Growth Cotton § 28.181 Review of cotton classification. A review of any classification or comparison made pursuant to this...

  13. 7 CFR 28.181 - Review of cotton classification.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 2 2012-01-01 2012-01-01 false Review of cotton classification. 28.181 Section 28.181... REGULATIONS COTTON CLASSING, TESTING, AND STANDARDS Classification for Foreign Growth Cotton § 28.181 Review of cotton classification. A review of any classification or comparison made pursuant to this...

  14. 19 CFR 181.11 - Certificate of Origin.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Certificate of Origin. 181.11 Section 181.11... TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Export Requirements § 181.11 Certificate of Origin. (a) General. A Certificate of Origin shall be employed to certify that a good being exported...

  15. 19 CFR 181.11 - Certificate of Origin.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Certificate of Origin. 181.11 Section 181.11... TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Export Requirements § 181.11 Certificate of Origin. (a) General. A Certificate of Origin shall be employed to certify that a good being exported...

  16. 19 CFR 181.11 - Certificate of Origin.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Certificate of Origin. 181.11 Section 181.11... TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Export Requirements § 181.11 Certificate of Origin. (a) General. A Certificate of Origin shall be employed to certify that a good being exported...

  17. 19 CFR 181.11 - Certificate of Origin.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Certificate of Origin. 181.11 Section 181.11... TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Export Requirements § 181.11 Certificate of Origin. (a) General. A Certificate of Origin shall be employed to certify that a good being exported...

  18. 19 CFR 181.101 - Publication of decisions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Advance Ruling Procedures § 181.101 Publication of.... Disclosure is governed by 31 CFR part 1, part 103 of this chapter, and § 181.99(a)(3) of this part. ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Publication of decisions. 181.101 Section...

  19. 21 CFR 820.181 - Device master record.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Device master record. 820.181 Section 820.181 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES QUALITY SYSTEM REGULATION Records § 820.181 Device master record. Each manufacturer shall...

  20. 14 CFR 29.181 - Dynamic stability: Category A rotorcraft.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 1 2013-01-01 2013-01-01 false Dynamic stability: Category A rotorcraft. 29.181 Section 29.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....181 Dynamic stability: Category A rotorcraft. Any short-period oscillation occurring at any speed...

  1. 14 CFR 29.181 - Dynamic stability: Category A rotorcraft.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 1 2012-01-01 2012-01-01 false Dynamic stability: Category A rotorcraft. 29.181 Section 29.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....181 Dynamic stability: Category A rotorcraft. Any short-period oscillation occurring at any speed...

  2. 14 CFR 29.181 - Dynamic stability: Category A rotorcraft.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Dynamic stability: Category A rotorcraft. 29.181 Section 29.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF....181 Dynamic stability: Category A rotorcraft. Any short-period oscillation occurring at any speed...

  3. 14 CFR 125.181 - Induction system ice prevention.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Induction system ice prevention. 125.181 Section 125.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION... Requirements § 125.181 Induction system ice prevention. A means for preventing the malfunctioning of...

  4. 1 CFR 18.1 - Original and copies required.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 1 General Provisions 1 2014-01-01 2012-01-01 true Original and copies required. 18.1 Section 18.1... PROCESSING OF DOCUMENTS PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.1 Original and copies... two duplicate originals or certified copies. 1 However, if the document is printed or processed...

  5. 1 CFR 18.1 - Original and copies required.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 1 General Provisions 1 2013-01-01 2012-01-01 true Original and copies required. 18.1 Section 18.1... PROCESSING OF DOCUMENTS PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.1 Original and copies... two duplicate originals or certified copies. 1 However, if the document is printed or processed...

  6. 1 CFR 18.1 - Original and copies required.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 1 General Provisions 1 2012-01-01 2012-01-01 false Original and copies required. 18.1 Section 18.1... PROCESSING OF DOCUMENTS PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.1 Original and copies... two duplicate originals or certified copies. 1 However, if the document is printed or processed...

  7. 14 CFR 91.181 - Course to be flown.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 2 2012-01-01 2012-01-01 false Course to be flown. 91.181 Section 91.181 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) AIR TRAFFIC... § 91.181 Course to be flown. Unless otherwise authorized by ATC, no person may operate an...

  8. 10 CFR 52.181 - Duration of renewal.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 2 2012-01-01 2012-01-01 false Duration of renewal. 52.181 Section 52.181 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSES, CERTIFICATIONS, AND APPROVALS FOR NUCLEAR POWER PLANTS Manufacturing Licenses § 52.181 Duration of renewal. A renewed manufacturing license may be issued for a term...

  9. 10 CFR 52.181 - Duration of renewal.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 2 2011-01-01 2011-01-01 false Duration of renewal. 52.181 Section 52.181 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSES, CERTIFICATIONS, AND APPROVALS FOR NUCLEAR POWER PLANTS Manufacturing Licenses § 52.181 Duration of renewal. A renewed manufacturing license may be issued for a term...

  10. 10 CFR 52.181 - Duration of renewal.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 2 2014-01-01 2014-01-01 false Duration of renewal. 52.181 Section 52.181 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSES, CERTIFICATIONS, AND APPROVALS FOR NUCLEAR POWER PLANTS Manufacturing Licenses § 52.181 Duration of renewal. A renewed manufacturing license may be issued for a term...

  11. 10 CFR 52.181 - Duration of renewal.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Duration of renewal. 52.181 Section 52.181 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSES, CERTIFICATIONS, AND APPROVALS FOR NUCLEAR POWER PLANTS Manufacturing Licenses § 52.181 Duration of renewal. A renewed manufacturing license may be issued for a term...

  12. 10 CFR 52.181 - Duration of renewal.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 2 2013-01-01 2013-01-01 false Duration of renewal. 52.181 Section 52.181 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSES, CERTIFICATIONS, AND APPROVALS FOR NUCLEAR POWER PLANTS Manufacturing Licenses § 52.181 Duration of renewal. A renewed manufacturing license may be issued for a term...

  13. 46 CFR 108.181 - Ventilation for enclosed spaces.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Ventilation for enclosed spaces. 108.181 Section 108.181... AND EQUIPMENT Construction and Arrangement Ventilation § 108.181 Ventilation for enclosed spaces. (a) Each enclosed space must be vented or ventilated. (b) There must be a means to close each vent...

  14. 46 CFR 108.181 - Ventilation for enclosed spaces.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Ventilation for enclosed spaces. 108.181 Section 108.181... AND EQUIPMENT Construction and Arrangement Ventilation § 108.181 Ventilation for enclosed spaces. (a) Each enclosed space must be vented or ventilated. (b) There must be a means to close each vent...

  15. 46 CFR 108.181 - Ventilation for enclosed spaces.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Ventilation for enclosed spaces. 108.181 Section 108.181... AND EQUIPMENT Construction and Arrangement Ventilation § 108.181 Ventilation for enclosed spaces. (a) Each enclosed space must be vented or ventilated. (b) There must be a means to close each vent...

  16. 46 CFR 108.181 - Ventilation for enclosed spaces.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Ventilation for enclosed spaces. 108.181 Section 108.181... AND EQUIPMENT Construction and Arrangement Ventilation § 108.181 Ventilation for enclosed spaces. (a) Each enclosed space must be vented or ventilated. (b) There must be a means to close each vent...

  17. 1 CFR 18.1 - Original and copies required.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 1 General Provisions 1 2011-01-01 2011-01-01 false Original and copies required. 18.1 Section 18.1... PROCESSING OF DOCUMENTS PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.1 Original and copies... two duplicate originals or certified copies. 1 However, if the document is printed or processed...

  18. 1 CFR 18.1 - Original and copies required.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 1 General Provisions 1 2010-01-01 2010-01-01 false Original and copies required. 18.1 Section 18.1... PROCESSING OF DOCUMENTS PREPARATION AND TRANSMITTAL OF DOCUMENTS GENERALLY § 18.1 Original and copies... two duplicate originals or certified copies. 1 However, if the document is printed or processed...

  19. 49 CFR 173.181 - Pyrophoric materials (liquids).

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 2 2014-10-01 2014-10-01 false Pyrophoric materials (liquids). 173.181 Section 173.181 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS... Class 7 § 173.181 Pyrophoric materials (liquids). When the § 172.101 table specifies that a...

  20. 49 CFR 173.181 - Pyrophoric materials (liquids).

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 2 2013-10-01 2013-10-01 false Pyrophoric materials (liquids). 173.181 Section 173.181 Transportation Other Regulations Relating to Transportation PIPELINE AND HAZARDOUS MATERIALS... Class 7 § 173.181 Pyrophoric materials (liquids). When the § 172.101 table specifies that a...

  1. MicroRNA 181b regulates decorin production by dermal fibroblasts and may be a potential therapy for hypertrophic scar.

    PubMed

    Kwan, Peter; Ding, Jie; Tredget, Edward E

    2015-01-01

    Hypertrophic scarring is a frequent fibroproliferative complication following deep dermal burns leading to impaired function and lifelong disfigurement. Decorin reduces fibrosis and induces regeneration in many tissues, and is significantly downregulated in hypertrophic scar and normal deep dermal fibroblasts. It was hypothesized that microRNAs in these fibroblasts downregulate decorin and blocking them would increase decorin and may prevent hypertrophic scarring. Lower decorin levels were found in hypertrophic scar as compared to normal skin, and in deep as compared to superficial dermis. A decorin 3' un-translated region reporter assay demonstrated microRNA decreased decorin in deep dermal fibroblasts, and microRNA screening predicted miR- 24, 181b, 421, 526b, or 543 as candidates. After finding increased levels of mir-181b in deep dermal fibroblasts, it was demonstrated that TGF-β1 stimulation decreased miR-24 but increased miR-181b and that hypertrophic scar and deep dermis contained increased levels of miR-181b. By blocking miR-181b with an antagomiR, it was possible to increase decorin protein expression in dermal fibroblasts. This suggests miR-181b is involved in the differential expression of decorin in skin and wound healing. Furthermore, blocking miR-181b reversed TGF-β1 induced decorin downregulation and myofibroblast differentiation in hypertrophic scar fibroblasts, suggesting a potential therapy for hypertrophic scar.

  2. 21 CFR 181.34 - Sodium nitrite and potassium nitrite.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Sodium nitrite and potassium nitrite. 181.34...-Sanctioned Food Ingredients § 181.34 Sodium nitrite and potassium nitrite. Sodium nitrite and potassium... fixatives and preservative agents, with or without sodium or potassium nitrate, in the curing of red...

  3. 21 CFR 181.34 - Sodium nitrite and potassium nitrite.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Sodium nitrite and potassium nitrite. 181.34...-Sanctioned Food Ingredients § 181.34 Sodium nitrite and potassium nitrite. Sodium nitrite and potassium... fixatives and preservative agents, with or without sodium or potassium nitrate, in the curing of red...

  4. 21 CFR 181.34 - Sodium nitrite and potassium nitrite.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Sodium nitrite and potassium nitrite. 181.34...-Sanctioned Food Ingredients § 181.34 Sodium nitrite and potassium nitrite. Sodium nitrite and potassium... fixatives and preservative agents, with or without sodium or potassium nitrate, in the curing of red...

  5. 26 CFR 1.181-6T - Effective date (temporary).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... first day of principal photography for which occurs on or after February 9, 2007, and before January 1...-between animation” in place of “principal photography”. Productions involving both animation and live-action photography may use either standard. (2) The applicability of §§ 1.181-1T through 1.181-5T...

  6. 26 CFR 1.181-6 - Effective/applicability date.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... paragraph (c) of this section, an owner may apply 26 CFR 1.181.1T through 1.181-5T (as contained in 26 CFR... productions, the first day of principal photography for which occurs on or after September 29, 2011. For an... “principal photography”. Productions involving both animation and live-action photography may use...

  7. 26 CFR 1.181-6 - Effective/applicability date.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ....181-1 through 1.181-5 apply to productions the first day of principal photography for which occurs on... principal photography or in-between animation occurs on or after December 7, 2012. (b) Pre-effective date... applies and for which the first day of principal photography (or in-between animation) occurred...

  8. 26 CFR 1.181-6 - Effective/applicability date.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ....181-1 through 1.181-5 apply to productions the first day of principal photography for which occurs on... principal photography or in-between animation occurs on or after December 7, 2012. (b) Pre-effective date... applies and for which the first day of principal photography (or in-between animation) occurred...

  9. 26 CFR 1.181-6T - Effective date (temporary).

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... first day of principal photography for which occurs on or after February 9, 2007, and before January 1...-between animation” in place of “principal photography”. Productions involving both animation and live-action photography may use either standard. (2) The applicability of §§ 1.181-1T through 1.181-5T...

  10. 27 CFR 24.181 - Use of sugar.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Use of sugar. 24.181... OF THE TREASURY LIQUORS WINE Production of Wine § 24.181 Use of sugar. Only sugar, as defined in § 24.10, may be used in the production of standard wine. The quantity of sugar used will be...

  11. 27 CFR 24.181 - Use of sugar.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Use of sugar. 24.181... OF THE TREASURY ALCOHOL WINE Production of Wine § 24.181 Use of sugar. Only sugar, as defined in § 24.10, may be used in the production of standard wine. The quantity of sugar used will be...

  12. 27 CFR 24.181 - Use of sugar.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Use of sugar. 24.181... OF THE TREASURY LIQUORS WINE Production of Wine § 24.181 Use of sugar. Only sugar, as defined in § 24.10, may be used in the production of standard wine. The quantity of sugar used will be...

  13. 27 CFR 24.181 - Use of sugar.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Use of sugar. 24.181... OF THE TREASURY LIQUORS WINE Production of Wine § 24.181 Use of sugar. Only sugar, as defined in § 24.10, may be used in the production of standard wine. The quantity of sugar used will be...

  14. 27 CFR 24.181 - Use of sugar.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Use of sugar. 24.181... OF THE TREASURY ALCOHOL WINE Production of Wine § 24.181 Use of sugar. Only sugar, as defined in § 24.10, may be used in the production of standard wine. The quantity of sugar used will be...

  15. 33 CFR 117.181 - Oakland Inner Harbor Tidal Canal.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Oakland Inner Harbor Tidal Canal. 117.181 Section 117.181 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY... Tidal Canal. The draws of the Alameda County highway drawbridges at Park Street, mile 5.2;...

  16. 30 CFR 250.181-250.185 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 2 2012-07-01 2012-07-01 false 250.181-250.185 Section 250.181-250.185 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF General Primary Lease Requirements, Lease...

  17. 30 CFR 250.181-250.185 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false 250.181-250.185 Section 250.181-250.185 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF General Primary Lease Requirements, Lease...

  18. 30 CFR 250.181-250.185 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 2 2014-07-01 2014-07-01 false 250.181-250.185 Section 250.181-250.185 Mineral Resources BUREAU OF SAFETY AND ENVIRONMENTAL ENFORCEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF General Primary Lease Requirements, Lease...

  19. 22 CFR 181.9 - Internet Web site publication.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Internet Web site publication. 181.9 Section... PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.9 Internet Web site publication. The Office of the Assistant... responsible for making publicly available on the Internet Web site of the Department of State each treaty...

  20. 22 CFR 181.9 - Internet Web site publication.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Internet Web site publication. 181.9 Section... PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.9 Internet Web site publication. The Office of the Assistant... responsible for making publicly available on the Internet Web site of the Department of State each treaty...

  1. 22 CFR 181.9 - Internet Web site publication.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Internet Web site publication. 181.9 Section... PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.9 Internet Web site publication. The Office of the Assistant... responsible for making publicly available on the Internet Web site of the Department of State each treaty...

  2. 22 CFR 181.9 - Internet Web site publication.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Internet Web site publication. 181.9 Section... PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.9 Internet Web site publication. The Office of the Assistant... responsible for making publicly available on the Internet Web site of the Department of State each treaty...

  3. 22 CFR 181.9 - Internet Web site publication.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Internet Web site publication. 181.9 Section... PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.9 Internet Web site publication. The Office of the Assistant... responsible for making publicly available on the Internet Web site of the Department of State each treaty...

  4. 49 CFR 192.181 - Distribution line valves.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... the flow or pressure of gas in a distribution system must have a valve installed on the inlet piping... 49 Transportation 3 2010-10-01 2010-10-01 false Distribution line valves. 192.181 Section 192.181... line valves. (a) Each high-pressure distribution system must have valves spaced so as to reduce...

  5. 49 CFR 192.181 - Distribution line valves.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... the flow or pressure of gas in a distribution system must have a valve installed on the inlet piping... 49 Transportation 3 2011-10-01 2011-10-01 false Distribution line valves. 192.181 Section 192.181... line valves. (a) Each high-pressure distribution system must have valves spaced so as to reduce...

  6. 46 CFR 108.181 - Ventilation for enclosed spaces.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Ventilation for enclosed spaces. 108.181 Section 108.181 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) A-MOBILE OFFSHORE DRILLING UNITS DESIGN... ventilating system. (c) Each fan in a ventilating system must have remote controls installed in...

  7. 27 CFR 19.181 - Automatic termination of permits.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... terminate. (c) Basic permits. The termination of basic permits is governed by the provisions of 27 CFR part... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Automatic termination of permits. 19.181 Section 19.181 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND...

  8. 21 CFR 181.32 - Acrylonitrile copolymers and resins.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Acrylonitrile copolymers and resins. 181.32...-Sanctioned Food Ingredients § 181.32 Acrylonitrile copolymers and resins. (a) Acrylonitrile copolymers and resins listed in this section, containing less than 30 percent acrylonitrile and complying with...

  9. 14 CFR 125.181 - Induction system ice prevention.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Induction system ice prevention. 125.181... Requirements § 125.181 Induction system ice prevention. A means for preventing the malfunctioning of each engine due to ice accumulation in the engine air induction system must be provided for each airplane....

  10. 14 CFR 125.181 - Induction system ice prevention.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Induction system ice prevention. 125.181... Requirements § 125.181 Induction system ice prevention. A means for preventing the malfunctioning of each engine due to ice accumulation in the engine air induction system must be provided for each airplane....

  11. 14 CFR 125.181 - Induction system ice prevention.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 3 2014-01-01 2014-01-01 false Induction system ice prevention. 125.181... Requirements § 125.181 Induction system ice prevention. A means for preventing the malfunctioning of each engine due to ice accumulation in the engine air induction system must be provided for each airplane....

  12. 14 CFR 125.181 - Induction system ice prevention.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Induction system ice prevention. 125.181... Requirements § 125.181 Induction system ice prevention. A means for preventing the malfunctioning of each engine due to ice accumulation in the engine air induction system must be provided for each airplane....

  13. 37 CFR 1.81 - Drawings required in patent application.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Drawings required in patent application. 1.81 Section 1.81 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL RULES OF PRACTICE IN PATENT CASES National Processing Provisions The...

  14. 37 CFR 1.81 - Drawings required in patent application.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2012-07-01 2012-07-01 false Drawings required in patent application. 1.81 Section 1.81 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL RULES OF PRACTICE IN PATENT CASES National Processing Provisions The...

  15. 37 CFR 1.81 - Drawings required in patent application.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2014-07-01 2014-07-01 false Drawings required in patent application. 1.81 Section 1.81 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL RULES OF PRACTICE IN PATENT CASES National Processing Provisions The...

  16. 37 CFR 1.81 - Drawings required in patent application.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2013-07-01 2013-07-01 false Drawings required in patent application. 1.81 Section 1.81 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL RULES OF PRACTICE IN PATENT CASES National Processing Provisions The...

  17. 37 CFR 1.81 - Drawings required in patent application.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2011-07-01 2011-07-01 false Drawings required in patent application. 1.81 Section 1.81 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF COMMERCE GENERAL RULES OF PRACTICE IN PATENT CASES National Processing Provisions The...

  18. 19 CFR 181.44 - Calculation of drawback.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Calculation of drawback. 181.44 Section 181.44... the United States from Japan, Company A paid $2.00 in duties. Company A manufactured the imported... Product X from Japan to the United States, Company A paid $5.00 in duties. Company A substituted a...

  19. 21 CFR 169.181 - Vanilla-vanillin flavoring.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Vanilla-vanillin flavoring. 169.181 Section 169... Dressings and Flavorings § 169.181 Vanilla-vanillin flavoring. (a) Vanilla-vanillin flavoring conforms to... ingredients prescribed for vanilla-vanillin extract by § 169.180, except that its content of ethyl alcohol...

  20. 21 CFR 169.181 - Vanilla-vanillin flavoring.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Vanilla-vanillin flavoring. 169.181 Section 169... Dressings and Flavorings § 169.181 Vanilla-vanillin flavoring. (a) Vanilla-vanillin flavoring conforms to... ingredients prescribed for vanilla-vanillin extract by § 169.180, except that its content of ethyl alcohol...

  1. 21 CFR 169.181 - Vanilla-vanillin flavoring.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Vanilla-vanillin flavoring. 169.181 Section 169... Dressings and Flavorings § 169.181 Vanilla-vanillin flavoring. (a) Vanilla-vanillin flavoring conforms to... ingredients prescribed for vanilla-vanillin extract by § 169.180, except that its content of ethyl alcohol...

  2. 21 CFR 169.181 - Vanilla-vanillin flavoring.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Vanilla-vanillin flavoring. 169.181 Section 169... Dressings and Flavorings § 169.181 Vanilla-vanillin flavoring. (a) Vanilla-vanillin flavoring conforms to... ingredients prescribed for vanilla-vanillin extract by § 169.180, except that its content of ethyl alcohol...

  3. 21 CFR 169.181 - Vanilla-vanillin flavoring.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Vanilla-vanillin flavoring. 169.181 Section 169... Dressings and Flavorings § 169.181 Vanilla-vanillin flavoring. (a) Vanilla-vanillin flavoring conforms to... ingredients prescribed for vanilla-vanillin extract by § 169.180, except that its content of ethyl alcohol...

  4. 19 CFR 181.75 - Issuance of origin determination.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Issuance of origin determination. 181.75 Section... OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Origin Verifications and Determinations § 181.75 Issuance of origin determination. (a) General. Except in the case of a pattern of...

  5. 19 CFR 181.75 - Issuance of origin determination.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Issuance of origin determination. 181.75 Section... OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Origin Verifications and Determinations § 181.75 Issuance of origin determination. (a) General. Except in the case of a pattern of...

  6. 19 CFR 181.76 - Application of origin determinations.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Application of origin determinations. 181.76...; DEPARTMENT OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Origin Verifications and Determinations § 181.76 Application of origin determinations. (a) General. Except as otherwise provided in...

  7. 19 CFR 181.76 - Application of origin determinations.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Application of origin determinations. 181.76...; DEPARTMENT OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Origin Verifications and Determinations § 181.76 Application of origin determinations. (a) General. Except as otherwise provided in...

  8. 19 CFR 181.75 - Issuance of origin determination.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Issuance of origin determination. 181.75 Section... OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Origin Verifications and Determinations § 181.75 Issuance of origin determination. (a) General. Except in the case of a pattern of...

  9. 19 CFR 181.76 - Application of origin determinations.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Application of origin determinations. 181.76...; DEPARTMENT OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Origin Verifications and Determinations § 181.76 Application of origin determinations. (a) General. Except as otherwise provided in...

  10. 19 CFR 181.76 - Application of origin determinations.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Application of origin determinations. 181.76...; DEPARTMENT OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Origin Verifications and Determinations § 181.76 Application of origin determinations. (a) General. Except as otherwise provided in...

  11. 21 CFR 181.33 - Sodium nitrate and potassium nitrate.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Sodium nitrate and potassium nitrate. 181.33...-Sanctioned Food Ingredients § 181.33 Sodium nitrate and potassium nitrate. Sodium nitrate and potassium... nitrite, with or without sodium or potassium nitrite, in the production of cured red meat products...

  12. 21 CFR 181.34 - Sodium nitrite and potassium nitrite.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Sodium nitrite and potassium nitrite. 181.34...-Sanctioned Food Ingredients § 181.34 Sodium nitrite and potassium nitrite. Sodium nitrite and potassium... fixatives and preservative agents, with or without sodium or potassium nitrate, in the curing of red...

  13. 19 CFR 10.181-10.182 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 1 2014-04-01 2014-04-01 false 10.181-10.182 Section 10.181-10.182 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. General Provisions Watches and Watch...

  14. 19 CFR 10.181-10.182 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 1 2012-04-01 2012-04-01 false 10.181-10.182 Section 10.181-10.182 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. General Provisions Watches and Watch...

  15. 19 CFR 10.181-10.182 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 1 2013-04-01 2013-04-01 false 10.181-10.182 Section 10.181-10.182 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. General Provisions Watches and Watch...

  16. 19 CFR 10.181-10.182 - [Reserved

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false 10.181-10.182 Section 10.181-10.182 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. General Provisions Watches and Watch...

  17. 19 CFR 10.181-10.182 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 1 2011-04-01 2011-04-01 false 10.181-10.182 Section 10.181-10.182 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. General Provisions Watches and Watch...

  18. 29 CFR 1915.181 - Electrical circuits and distribution boards.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 7 2012-07-01 2012-07-01 false Electrical circuits and distribution boards. 1915.181... Electrical Machinery § 1915.181 Electrical circuits and distribution boards. (a) The provisions of this... employee is permitted to work on an electrical circuit, except when the circuit must remain energized...

  19. 29 CFR 1915.181 - Electrical circuits and distribution boards.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 7 2014-07-01 2014-07-01 false Electrical circuits and distribution boards. 1915.181... Electrical Machinery § 1915.181 Electrical circuits and distribution boards. (a) The provisions of this... employee is permitted to work on an electrical circuit, except when the circuit must remain energized...

  20. 21 CFR 181.32 - Acrylonitrile copolymers and resins.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Acrylonitrile copolymers and resins. 181.32 Section... Ingredients § 181.32 Acrylonitrile copolymers and resins. (a) Acrylonitrile copolymers and resins listed in... of the vinyl chloride resin) resin—for use only in contact with oleomargarine. (iv)...

  1. 46 CFR 181.310 - Fire main and hydrants.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Fire main and hydrants. 181.310 Section 181.310 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS... power driven fire pump must have a sufficient number of fire hydrants to reach any part of the...

  2. 46 CFR 181.310 - Fire main and hydrants.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Fire main and hydrants. 181.310 Section 181.310 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS... power driven fire pump must have a sufficient number of fire hydrants to reach any part of the...

  3. 46 CFR 181.310 - Fire main and hydrants.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Fire main and hydrants. 181.310 Section 181.310 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS... power driven fire pump must have a sufficient number of fire hydrants to reach any part of the...

  4. 46 CFR 181.310 - Fire main and hydrants.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Fire main and hydrants. 181.310 Section 181.310 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) SMALL PASSENGER VESSELS (UNDER 100 GROSS TONS... power driven fire pump must have a sufficient number of fire hydrants to reach any part of the...

  5. 19 CFR 181.95 - Oral discussion of issues.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Oral discussion of issues. 181.95 Section 181.95... of issues. (a) General. A person submitting a request for an advance ruling and desiring an opportunity to orally discuss the issue or issues involved should indicate that desire in writing at the...

  6. 9 CFR 319.181 - Cheesefurters and similar products.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 2 2010-01-01 2010-01-01 false Cheesefurters and similar products. 319.181 Section 319.181 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY ORGANIZATION AND TERMINOLOGY; MANDATORY MEAT AND POULTRY PRODUCTS INSPECTION AND...

  7. 46 CFR 181.320 - Fire hoses and nozzles.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... fire hose that conforms to UL 19 (incorporated by reference, see 46 CFR 175.600) or hose that is listed... by reference, see 46 CFR 175.600) or other standard specified by the Commandant. (c) Each fire hose... 46 Shipping 7 2011-10-01 2011-10-01 false Fire hoses and nozzles. 181.320 Section 181.320...

  8. 46 CFR 181.320 - Fire hoses and nozzles.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... fire hose that conforms to UL 19 (incorporated by reference, see 46 CFR 175.600) or hose that is listed... by reference, see 46 CFR 175.600) or other standard specified by the Commandant. (c) Each fire hose... 46 Shipping 7 2012-10-01 2012-10-01 false Fire hoses and nozzles. 181.320 Section 181.320...

  9. 7 CFR 1260.181 - Qualified State beef councils.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 10 2010-01-01 2010-01-01 false Qualified State beef councils. 1260.181 Section 1260... SERVICE (MARKETING AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE BEEF PROMOTION AND RESEARCH Beef Promotion and Research Order Assessments § 1260.181 Qualified State...

  10. 21 CFR 181.32 - Acrylonitrile copolymers and resins.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Acrylonitrile copolymers and resins. 181.32 Section 181.32 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  11. 29 CFR 1915.181 - Electrical circuits and distribution boards.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 7 2011-07-01 2011-07-01 false Electrical circuits and distribution boards. 1915.181... Electrical Machinery § 1915.181 Electrical circuits and distribution boards. (a) The provisions of this... employee is permitted to work on an electrical circuit, except when the circuit must remain energized...

  12. 7 CFR 1150.181 - Proceedings after termination.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 9 2013-01-01 2013-01-01 false Proceedings after termination. 1150.181 Section 1150.181 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MILK), DEPARTMENT OF AGRICULTURE DAIRY PROMOTION PROGRAM...

  13. 7 CFR 1150.181 - Proceedings after termination.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Proceedings after termination. 1150.181 Section 1150.181 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Milk), DEPARTMENT OF AGRICULTURE DAIRY PROMOTION PROGRAM...

  14. 7 CFR 1150.181 - Proceedings after termination.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 9 2012-01-01 2012-01-01 false Proceedings after termination. 1150.181 Section 1150.181 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Milk), DEPARTMENT OF AGRICULTURE DAIRY PROMOTION PROGRAM...

  15. 7 CFR 1150.181 - Proceedings after termination.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 9 2014-01-01 2013-01-01 true Proceedings after termination. 1150.181 Section 1150.181 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING AGREEMENTS AND ORDERS; MILK), DEPARTMENT OF AGRICULTURE DAIRY PROMOTION PROGRAM...

  16. 33 CFR 181.17 - Label numbers and letters.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Label numbers and letters. 181.17...) BOATING SAFETY MANUFACTURER REQUIREMENTS Manufacturer Certification of Compliance § 181.17 Label numbers and letters. Letters and numbers on each label must: (a) Be no less than one-eighth of an inch...

  17. 33 CFR 181.25 - Hull identification number format.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Hull identification number format... number format. Each of the hull identification numbers required by § 181.23 must consist of twelve... under § 181.31(b). (b) Characters four through eight must be a serial number assigned by...

  18. 33 CFR 181.35 - Removal of numbers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Removal of numbers. 181.35...) BOATING SAFETY MANUFACTURER REQUIREMENTS Identification of Boats § 181.35 Removal of numbers. No person may remove or alter a number required by this subpart unless authorized by the Commandant, U.S....

  19. 21 CFR 181.33 - Sodium nitrate and potassium nitrate.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Sodium nitrate and potassium nitrate. 181.33...-Sanctioned Food Ingredients § 181.33 Sodium nitrate and potassium nitrate. Sodium nitrate and potassium nitrate are subject to prior sanctions issued by the U.S. Department of Agriculture for use as sources...

  20. 21 CFR 181.33 - Sodium nitrate and potassium nitrate.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Sodium nitrate and potassium nitrate. 181.33 Section 181.33 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES... nitrate and potassium nitrate. Sodium nitrate and potassium nitrate are subject to prior sanctions...

  1. 21 CFR 181.33 - Sodium nitrate and potassium nitrate.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Sodium nitrate and potassium nitrate. 181.33...-Sanctioned Food Ingredients § 181.33 Sodium nitrate and potassium nitrate. Sodium nitrate and potassium nitrate are subject to prior sanctions issued by the U.S. Department of Agriculture for use as sources...

  2. 21 CFR 181.33 - Sodium nitrate and potassium nitrate.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Sodium nitrate and potassium nitrate. 181.33...-Sanctioned Food Ingredients § 181.33 Sodium nitrate and potassium nitrate. Sodium nitrate and potassium nitrate are subject to prior sanctions issued by the U.S. Department of Agriculture for use as sources...

  3. 49 CFR 173.181 - Pyrophoric materials (liquids).

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 2 2012-10-01 2012-10-01 false Pyrophoric materials (liquids). 173.181 Section... Class 7 § 173.181 Pyrophoric materials (liquids). When the § 172.101 table specifies that a hazardous... pyrophoric liquids may not exceed two-thirds of the rated capacity of the outer drum. For example, a 220...

  4. TGF-β Controls miR-181/ERK Regulatory Network during Retinal Axon Specification and Growth

    PubMed Central

    Carrella, Sabrina; Salierno, Francesco Giuseppe; Manfredi, Anna; Banfi, Sandro; Conte, Ivan

    2015-01-01

    Retinal axon specification and growth are critically sensitive to the dosage of numerous signaling molecules and transcription factors. Subtle variations in the expression levels of key molecules may result in a variety of axonal growth anomalies. miR-181a and miR-181b are two eye-enriched microRNAs whose inactivation in medaka fish leads to alterations of the proper establishment of connectivity and function in the visual system. miR-181a/b are fundamental regulators of MAPK signaling and their role in retinal axon growth and specification is just beginning to be elucidated. Here we demonstrate that miR-181a/b are key nodes in the interplay between TGF-β and MAPK/ERK within the functional pathways that control retinal axon specification and growth. Using a variety of in vivo and in vitro approaches in medaka fish, we demonstrate that TGF-β signaling controls the miR-181/ERK regulatory network, which in turn strengthens the TGF-β-mediated regulation of RhoA degradation. Significantly, these data uncover the role of TGF-β signaling in vivo, for the first time, in defining the correct wiring and assembly of functional retina neural circuits and further highlight miR-181a/b as key factors in axon specification and growth. PMID:26641497

  5. The DELTA 181 lithium thionyl chloride battery

    NASA Astrophysics Data System (ADS)

    Sullivan, Ralph M.; Brown, Lawrence E.; Leigh, A. P.

    In 1986, the Johns Hopkins University/Applied Physics Laboratory (JHU/APL) undertook the development of a sensor module for the DELTA 181 spacecraft, a low earth orbit (LEO) mission of less than two months duration. A large lithium thionyl chloride battery was developed as the spacecraft's primary power source, the first known such use for this technology. The exceptionally high energy density of the lithium thionyl chloride cell was the primary driver for its use, resulting in a completed battery with a specific energy density of 120 Wh/lb. Safety requirements became the primary driver shaping all aspects of the power system design and development due to concerns about the potential hazards of this relatively new, high-energy technology. However, the program was completed without incident. The spacecraft was launched on February 8, 1988, from Kennedy Space Center (KSC) with over 60,000 Wh of battery energy. It reentered on April 2, 1988, still operating after 55 days, providing a successful, practical, and visible demonstration of the use of this technology for spacecraft applications.

  6. 40 CFR 60.181 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... sinter as it is discharged from the conveying grate of a sintering machine. (e) Blast furnace means any reduction furnace to which sinter is charged and which forms separate layers of molten slag and lead bullion... pyrometallurgical techniques. (b) Sintering machine means any furnace in which a lead sulfide ore concentrate...

  7. 40 CFR 60.181 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... sinter as it is discharged from the conveying grate of a sintering machine. (e) Blast furnace means any reduction furnace to which sinter is charged and which forms separate layers of molten slag and lead bullion... pyrometallurgical techniques. (b) Sintering machine means any furnace in which a lead sulfide ore concentrate...

  8. 40 CFR 60.181 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... sinter as it is discharged from the conveying grate of a sintering machine. (e) Blast furnace means any reduction furnace to which sinter is charged and which forms separate layers of molten slag and lead bullion... pyrometallurgical techniques. (b) Sintering machine means any furnace in which a lead sulfide ore concentrate...

  9. 40 CFR 60.181 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... sinter as it is discharged from the conveying grate of a sintering machine. (e) Blast furnace means any reduction furnace to which sinter is charged and which forms separate layers of molten slag and lead bullion... pyrometallurgical techniques. (b) Sintering machine means any furnace in which a lead sulfide ore concentrate...

  10. 40 CFR 60.181 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... sinter as it is discharged from the conveying grate of a sintering machine. (e) Blast furnace means any reduction furnace to which sinter is charged and which forms separate layers of molten slag and lead bullion... pyrometallurgical techniques. (b) Sintering machine means any furnace in which a lead sulfide ore concentrate...

  11. 26 CFR 1.181-4 - Special rules.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... before computing gain or loss from the disposition. (2) Principal photography not commencing prior to the... for which principal photography does not commence prior to the date of expiration of section 181,...

  12. 26 CFR 1.181-4 - Special rules.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... before computing gain or loss from the disposition. (2) Principal photography not commencing prior to the... for which principal photography does not commence prior to the date of expiration of section 181,...

  13. 26 CFR 1.181-4 - Special rules.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... before computing gain or loss from the disposition. (2) Principal photography not commencing prior to the... for which principal photography does not commence prior to the date of expiration of section 181,...

  14. 19 CFR 181.93 - Submission of advance ruling requests.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... English language and in the form of a letter. For any subject matter specified in § 181.92(b)(6)(i), (v... quasi-judicial body in Canada or Mexico; (2) A verification of origin performed in the United...

  15. 19 CFR 181.93 - Submission of advance ruling requests.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... English language and in the form of a letter. For any subject matter specified in § 181.92(b)(6)(i), (v... quasi-judicial body in Canada or Mexico; (2) A verification of origin performed in the United...

  16. 9 CFR 319.181 - Cheesefurters and similar products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... INSPECTION AND CERTIFICATION DEFINITIONS AND STANDARDS OF IDENTITY OR COMPOSITION Cooked Sausage § 319.181... combination of fat and added water, and no more than 30 percent fat and shall comply with the other...

  17. 47 CFR 0.181 - The Defense Commissioner.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... in public safety, homeland security, national security, emergency preparedness, disaster management..., Defense and Emergency Preparedness Functions § 0.181 The Defense Commissioner. The Defense Commissioner is... emergency preparedness, and defense activities of the Commission and has the following duties...

  18. 47 CFR 0.181 - The Defense Commissioner.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... in public safety, homeland security, national security, emergency preparedness, disaster management..., Defense and Emergency Preparedness Functions § 0.181 The Defense Commissioner. The Defense Commissioner is... emergency preparedness, and defense activities of the Commission and has the following duties...

  19. 47 CFR 0.181 - The Defense Commissioner.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... in public safety, homeland security, national security, emergency preparedness, disaster management..., Defense and Emergency Preparedness Functions § 0.181 The Defense Commissioner. The Defense Commissioner is... emergency preparedness, and defense activities of the Commission and has the following duties...

  20. 47 CFR 0.181 - The Defense Commissioner.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... in public safety, homeland security, national security, emergency preparedness, disaster management..., Defense and Emergency Preparedness Functions § 0.181 The Defense Commissioner. The Defense Commissioner is... emergency preparedness, and defense activities of the Commission and has the following duties...

  1. 47 CFR 0.181 - The Defense Commissioner.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... in public safety, homeland security, national security, emergency preparedness, disaster management..., Defense and Emergency Preparedness Functions § 0.181 The Defense Commissioner. The Defense Commissioner is... emergency preparedness, and defense activities of the Commission and has the following duties...

  2. 181. VIEW OF WEST END OF SMELTER COMPLEX, SHOWING (LEFT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    181. VIEW OF WEST END OF SMELTER COMPLEX, SHOWING (LEFT TO RIGHT) COOPER SHOP(1898); LUMBER SHED (C. 1917); COOPER STOCKHOUSE (1898) AND MACHINE SHOP (1907). - Quincy Mining Company, Hancock, Houghton County, MI

  3. 19 CFR 18.1 - Carriers; application to bond.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... Editorial Note: For Federal Register citations affecting § 18.1, see the List of CFR Sections Affected...” means a common carrier of merchandise owning or operating a railroad, steamship, pipeline, or...

  4. Atomic Mass and Nuclear Binding Energy for I-181 (Iodine)

    NASA Astrophysics Data System (ADS)

    Sukhoruchkin, S. I.; Soroko, Z. N.

    This document is part of the Supplement containing the complete sets of data of Subvolume A `Nuclei with Z = 1 - 54' of Volume 22 `Nuclear Binding Energies and Atomic Masses' of Landolt-Börnstein - Group I `Elementary Particles, Nuclei and Atoms'. It provides atomic mass, mass excess, nuclear binding energy, nucleon separation energies, Q-values, and nucleon residual interaction parameters for atomic nuclei of the isotope I-181 (Iodine, atomic number Z = 53, mass number A = 181).

  5. miR‐181a/b control the assembly of visual circuitry by regulating retinal axon specification and growth

    PubMed Central

    Carrella, Sabrina; D'Agostino, Ylenia; Barbato, Sara; Huber‐Reggi, Sabina P.; Salierno, Francesco G.; Manfredi, Anna; Neuhauss, Stephan C.F.

    2015-01-01

    ABSTRACT Connectivity and function of neuronal circuitry require the correct specification and growth of axons and dendrites. Here, we identify the microRNAs miR‐181a and miR‐181b as key regulators of retinal axon specification and growth. Loss of miR‐181a/b in medaka fish (Oryzias latipes) failed to consolidate amacrine cell processes into axons and delayed the growth of retinal ganglion cell (RGC) axons. These alterations were accompanied by defects in visual connectivity and function. We demonstrated that miR‐181a/b exert these actions through negative modulation of MAPK/ERK signaling that in turn leads to RhoA reduction and proper neuritogenesis in both amacrine cells and RGCs via local cytoskeletal rearrangement. Our results identify a new pathway for axon specification and growth unraveling a crucial role of miR‐181a/b in the proper establishment of visual system connectivity and function. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 75: 1252–1267, 2015 PMID:25728313

  6. Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF181

    PubMed Central

    Pedersen, Sarah M.; Chan, Waipan; Jattani, Rakhi P.; Mackie, deMauri S.

    2015-01-01

    NF-κB activation downstream of antigen receptor engagement is a highly regulated event required for lymphocyte activation during the adaptive immune response. The pathway is often dysregulated in lymphoma, leading to constitutive NF-κB activity that supports the aberrant proliferation of transformed lymphocytes. To identify novel regulators of antigen receptor signaling to NF-κB, we developed bioluminescence resonance energy transfer-based interaction cloning (BRIC), a screening strategy that can detect protein-protein interactions in live mammalian cells in a high-throughput manner. Using this strategy, we identified the RING finger protein RNF181 as an interactor of CARD11, a key signaling scaffold in the antigen receptor pathway. We present evidence that RNF181 functions as an E3 ubiquitin ligase to inhibit antigen receptor signaling to NF-κB downstream of CARD11. The levels of the obligate signaling protein Bcl10 are reduced by RNF181 even prior to signaling, and Bcl10 can serve as a substrate for RNF181 E3 ligase activity in vitro. Furthermore, RNF181 limits the proliferation of human diffuse large B cell lymphoma cells that depend upon aberrant CARD11 signaling to NF-κB for growth and survival in culture. Our results define a new regulatory checkpoint that can modulate the output of CARD11 signaling to NF-κB in both normal and transformed lymphocytes. PMID:26711259

  7. 22 CFR 181.1 - Purpose and application.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... affect the legal validity, under United States law or under international law, of agreements concluded... Foreign Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.1 Purpose and application. (a) The purpose of this part is to implement...

  8. 22 CFR 181.1 - Purpose and application.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... affect the legal validity, under United States law or under international law, of agreements concluded... Foreign Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.1 Purpose and application. (a) The purpose of this part is to implement...

  9. 22 CFR 181.1 - Purpose and application.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... affect the legal validity, under United States law or under international law, of agreements concluded... Foreign Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.1 Purpose and application. (a) The purpose of this part is to implement...

  10. 22 CFR 181.1 - Purpose and application.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... affect the legal validity, under United States law or under international law, of agreements concluded... Foreign Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.1 Purpose and application. (a) The purpose of this part is to implement...

  11. 22 CFR 181.1 - Purpose and application.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... affect the legal validity, under United States law or under international law, of agreements concluded... Foreign Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.1 Purpose and application. (a) The purpose of this part is to implement...

  12. 21 CFR 181.34 - Sodium nitrite and potassium nitrite.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Sodium nitrite and potassium nitrite. 181.34... nitrite and potassium nitrite. Sodium nitrite and potassium nitrite are subject to prior sanctions issued... without sodium or potassium nitrate, in the curing of red meat and poultry products....

  13. 12 CFR 18.1 - Purpose and OMB control number.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... statement with narrative information management deems important. The availability of this information is... AND OTHER INFORMATION BY NATIONAL BANKS § 18.1 Purpose and OMB control number. (a) Purpose. The... public confidence in the national banking system. (b) OMB control number. The collection of...

  14. 13 CFR 120.181 - Status of Lenders and CDCs.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Status of Lenders and CDCs. 120....181 Status of Lenders and CDCs. Lenders, CDCs and their contractors are independent contractors that... wrongful action taken by a Lender, CDC or an employee, agent, or contractor of a Lender or CDC....

  15. 13 CFR 120.181 - Status of Lenders and CDCs.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Status of Lenders and CDCs. 120....181 Status of Lenders and CDCs. Lenders, CDCs and their contractors are independent contractors that... wrongful action taken by a Lender, CDC or an employee, agent, or contractor of a Lender or CDC....

  16. 13 CFR 120.181 - Status of Lenders and CDCs.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Status of Lenders and CDCs. 120....181 Status of Lenders and CDCs. Lenders, CDCs and their contractors are independent contractors that... wrongful action taken by a Lender, CDC or an employee, agent, or contractor of a Lender or CDC....

  17. 13 CFR 120.181 - Status of Lenders and CDCs.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Status of Lenders and CDCs. 120....181 Status of Lenders and CDCs. Lenders, CDCs and their contractors are independent contractors that... wrongful action taken by a Lender, CDC or an employee, agent, or contractor of a Lender or CDC....

  18. 13 CFR 120.181 - Status of Lenders and CDCs.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Status of Lenders and CDCs. 120....181 Status of Lenders and CDCs. Lenders, CDCs and their contractors are independent contractors that... wrongful action taken by a Lender, CDC or an employee, agent, or contractor of a Lender or CDC....

  19. 181. Photocopy of drawing (1973 architectural drawing by the Space ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    181. Photocopy of drawing (1973 architectural drawing by the Space and Missile Test Center, VAFB, USAF) SITE PLAN, SECTIONS, AND DETAILS FOR THE FUEL TANK RETAINING WALL, SHEET 27A OF 53 - Vandenberg Air Force Base, Space Launch Complex 3, Launch Pad 3 West, Napa & Alden Roads, Lompoc, Santa Barbara County, CA

  20. 19 CFR 181.75 - Issuance of origin determination.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... within the meaning of § 181.76(c) of this part, following receipt and analysis of the results of an... calendar days after conclusion of the origin verification process, unless circumstances require additional... determination where specific findings of fact cannot be made because of a failure to respond to a...

  1. 181. Photographic copy of original construction drawing dated March 28, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    181. Photographic copy of original construction drawing dated March 28, 1932 (from Record Group 115, Denver Branch of the National Archives, Denver). VOLUME CHANGE IN MASS CONCRETE; OWYHEE DAM CONCRETE RESEARCH FOR HOOVER DAM; LOCATION OF TERMINAL BOARDS AND CONDUCTS. - Owyhee Dam, Across Owyhee River, Nyssa, Malheur County, OR

  2. 25 CFR 181.5 - How are applications ranked?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAND AND WATER INDIAN HIGHWAY SAFETY PROGRAM § 181.5... four factors. (a) Factor No. 1—Magnitude of the problem (Up to 50 points available). In awarding points under this factor, BIA will take into account the following: (1) Whether a highway safety problem...

  3. 33 CFR 181.31 - Manufacturer identification code assignment.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... SECURITY (CONTINUED) BOATING SAFETY MANUFACTURER REQUIREMENTS Identification of Boats § 181.31 Manufacturer... request a manufacturer identification code in writing from the Recreational Boating Product Assurance... the Recreational Boating Product Assurance Branch (CG-54223), 2100 2nd St., SW., Stop 7581,...

  4. 33 CFR 181.31 - Manufacturer identification code assignment.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... SECURITY (CONTINUED) BOATING SAFETY MANUFACTURER REQUIREMENTS Identification of Boats § 181.31 Manufacturer... request a manufacturer identification code in writing from the Recreational Boating Product Assurance... the Recreational Boating Product Assurance Branch (CG-54223), 2100 2nd St., SW., Stop 7581,...

  5. 17 CFR 256.181 - Unamortized debt expense.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 3 2011-04-01 2011-04-01 false Unamortized debt expense. 256... HOLDING COMPANY ACT OF 1935 4. Deferred Debits § 256.181 Unamortized debt expense. This account shall include expenses related to the issuance or assumption of debt securities. Amounts recorded in...

  6. 17 CFR 256.181 - Unamortized debt expense.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 3 2010-04-01 2010-04-01 false Unamortized debt expense. 256... HOLDING COMPANY ACT OF 1935 4. Deferred Debits § 256.181 Unamortized debt expense. This account shall include expenses related to the issuance or assumption of debt securities. Amounts recorded in...

  7. 18 CFR 367.1810 - Account 181, Unamortized debt expense.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Unamortized debt expense. 367.1810 Section 367.1810 Conservation of Power and Water Resources FEDERAL ENERGY... GAS ACT Balance Sheet Chart of Accounts Deferred Debits § 367.1810 Account 181, Unamortized debt expense. This account must include expenses related to the issuance or assumption of debt...

  8. 46 CFR 181.425 - Galley hood fire extinguishing systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... meeting the applicable sections of NFPA 17 or NFPA 17A (both incorporated by reference, see 46 CFR 175.600... Galley hood fire extinguishing systems. (a) A grease extraction hood required by 46 CFR 181.400 must meet UL 710 (incorporated by reference, see 46 CFR 175.600) or other standard specified by the...

  9. 46 CFR 181.425 - Galley hood fire extinguishing systems.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... meeting the applicable sections of NFPA 17 or NFPA 17A (both incorporated by reference, see 46 CFR 175.600... Galley hood fire extinguishing systems. (a) A grease extraction hood required by 46 CFR 181.400 must meet UL 710 (incorporated by reference, see 46 CFR 175.600) or other standard specified by the...

  10. 46 CFR 181.425 - Galley hood fire extinguishing systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... meeting the applicable sections of NFPA 17 or NFPA 17A (both incorporated by reference, see 46 CFR 175.600... Galley hood fire extinguishing systems. (a) A grease extraction hood required by 46 CFR 181.400 must meet UL 710 (incorporated by reference, see 46 CFR 175.600) or other standard specified by the...

  11. 14 CFR 29.181 - Dynamic stability: Category A rotorcraft.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 1 2011-01-01 2011-01-01 false Dynamic stability: Category A rotorcraft... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Flight Flight Characteristics § 29.181 Dynamic stability: Category A rotorcraft. Any short-period oscillation occurring at any speed...

  12. 14 CFR 29.181 - Dynamic stability: Category A rotorcraft.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false Dynamic stability: Category A rotorcraft... TRANSPORTATION AIRCRAFT AIRWORTHINESS STANDARDS: TRANSPORT CATEGORY ROTORCRAFT Flight Flight Characteristics § 29.181 Dynamic stability: Category A rotorcraft. Any short-period oscillation occurring at any speed...

  13. 21 CFR 181.32 - Acrylonitrile copolymers and resins.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.32 Acrylonitrile copolymers and resins. (a) Acrylonitrile copolymers and... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Acrylonitrile copolymers and resins....

  14. 21 CFR 181.32 - Acrylonitrile copolymers and resins.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.32 Acrylonitrile copolymers and resins. (a) Acrylonitrile copolymers and... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Acrylonitrile copolymers and resins....

  15. 19 CFR 181.12 - Maintenance and availability of records.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...; DEPARTMENT OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT Export Requirements § 181.12... claimed in Canada or Mexico, including records associated with: (i) The purchase of, cost of, value of, and payment for, the good that is exported from the United States; (ii) The purchase of, cost...

  16. 33 CFR 181.23 - Hull identification numbers required.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Hull identification numbers... identification numbers required. (a) A manufacturer (or importer), as defined in § 181.3 of this part, must identify each boat produced or imported with two hull identification numbers that meet the requirements...

  17. 27 CFR 31.181 - Requirements for retail dealers.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Requirements for retail... BUREAU, DEPARTMENT OF THE TREASURY LIQUORS ALCOHOL BEVERAGE DEALERS Records and Reports Retail Dealer's Records § 31.181 Requirements for retail dealers. (a) Records of receipt. All retail dealers must keep...

  18. 27 CFR 31.181 - Requirements for retail dealers.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Requirements for retail... BUREAU, DEPARTMENT OF THE TREASURY LIQUORS ALCOHOL BEVERAGE DEALERS Records and Reports Retail Dealer's Records § 31.181 Requirements for retail dealers. (a) Records of receipt. All retail dealers must keep...

  19. 37 CFR 1.181 - Petition to the Director.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Petition to the Director. 1... Action by the Director § 1.181 Petition to the Director. (a) Petition may be taken to the Director: (1... rules specify that the matter is to be determined directly by or reviewed by the Director; and (3)...

  20. 37 CFR 1.181 - Petition to the Director.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2011-07-01 2011-07-01 false Petition to the Director. 1... Action by the Director § 1.181 Petition to the Director. (a) Petition may be taken to the Director: (1... rules specify that the matter is to be determined directly by or reviewed by the Director; and (3)...

  1. 37 CFR 1.181 - Petition to the Director.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2012-07-01 2012-07-01 false Petition to the Director. 1... Action by the Director § 1.181 Petition to the Director. (a) Petition may be taken to the Director: (1... rules specify that the matter is to be determined directly by or reviewed by the Director; and (3)...

  2. 37 CFR 1.181 - Petition to the Director.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2013-07-01 2013-07-01 false Petition to the Director. 1... Action by the Director § 1.181 Petition to the Director. (a) Petition may be taken to the Director: (1... the matter is to be determined directly by or reviewed by the Director; and (3) To invoke...

  3. 37 CFR 1.181 - Petition to the Director.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2014-07-01 2014-07-01 false Petition to the Director. 1... Action by the Director § 1.181 Petition to the Director. (a) Petition may be taken to the Director: (1... the matter is to be determined directly by or reviewed by the Director; and (3) To invoke...

  4. 46 CFR 181.425 - Galley hood fire extinguishing systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Galley hood fire extinguishing systems. (a) A grease extraction hood required by 46 CFR 181.400 must meet UL 710 (incorporated by reference, see 46 CFR 175.600) or other standard specified by the Commandant... meeting the applicable sections of NFPA 17 or NFPA 17A (both incorporated by reference, see 46 CFR...

  5. 46 CFR 181.425 - Galley hood fire extinguishing systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Galley hood fire extinguishing systems. (a) A grease extraction hood required by 46 CFR 181.400 must meet UL 710 (incorporated by reference, see 46 CFR 175.600) or other standard specified by the Commandant... meeting the applicable sections of NFPA 17 or NFPA 17A (both incorporated by reference, see 46 CFR...

  6. 12 CFR 18.1 - Purpose and OMB control number.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... statement with narrative information management deems important. The availability of this information is... AND OTHER INFORMATION BY NATIONAL BANKS § 18.1 Purpose and OMB control number. (a) Purpose. The... public confidence in the national banking system. (b) OMB control number. The collection of...

  7. 12 CFR 18.1 - Purpose and OMB control number.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... statement with narrative information management deems important. The availability of this information is... AND OTHER INFORMATION BY NATIONAL BANKS § 18.1 Purpose and OMB control number. (a) Purpose. The... public confidence in the national banking system. (b) OMB control number. The collection of...

  8. 12 CFR 18.1 - Purpose and OMB control number.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... statement with narrative information management deems important. The availability of this information is... AND OTHER INFORMATION BY NATIONAL BANKS § 18.1 Purpose and OMB control number. (a) Purpose. The... public confidence in the national banking system. (b) OMB control number. The collection of...

  9. Identification of Cellular Targets of MicroRNA-181a in HepG2 Cells: A New Approach for Functional Analysis of MicroRNAs

    PubMed Central

    Tan, Jane Yi Lin; Habib, Nagy A.; Chuah, York Wieo; Yau, Yin Hoe; Geifman-Shochat, Susana; Chen, Wei Ning

    2015-01-01

    MicroRNAs (miRNAs) are known to play a part in regulating important cellular processes. They generally perform their regulatory function through their binding with mRNAs, ultimately leading to a repression of target protein expression levels. However, their roles in cellular processes are poorly understood due to the limited understanding of their specific cellular targets. Aberrant levels of miRNAs have been found in hepatocellular carcinoma (HCC) including miR-181a. Using bioinformatics analysis, cyclin-dependent kinase inhibitor 1B (CDKN1β) and transcriptional factor E2F7 were identified as potential targets of miR-181a. Validation analysis using surface plasmon resonance (SPR) showed a positive binding between miR-181a and the 3’UTRs of these two potential mRNA targets. In vivo luciferase assay further confirmed the positive miR-181a:mRNA bindings, where a significant decrease in luciferase activity was detected when HepG2 cells were co-transfected with the 3’UTR-containing reporter plasmids and miR-181a. The potential impact of miR-181a binding to its specific targets on the general cellular behavior was further investigated. Results showed that miR-181a significantly activated the MAPK/JNK pathway which regulates cell proliferation, supporting our recently reported findings. Inhibition of miR-181a, on the other hand, abolished the observed activation. Our findings open up a new approach in designing targeted functional analysis of miRNAs in cellular processes, through the identification of their cellular targets. PMID:25901570

  10. 142. North Carolina route 181 grade separation structure. This single ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    142. North Carolina route 181 grade separation structure. This single span, reinforced concrete, elliptical, spandrel arch structure, built in 1959, is on a slight skew as it passes over the parkway accounting for its wide span of 80. It is one of the few structures to pass over the parkway. This view of the elevation is to the north. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  11. 257. North Carolina Route 181 grade separation structure is a ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    257. North Carolina Route 181 grade separation structure is a single-span, reinforced concrete, elliptical spandrel arch structure built in 1959, it is on a slight skew as it passes over the parkway accounting for its wide span of 80. It is one of the few structures to pass over the parkway. This view is to the north. - Blue Ridge Parkway, Between Shenandoah National Park & Great Smoky Mountains, Asheville, Buncombe County, NC

  12. 26 CFR 1.181-0T - Table of contents (temporary).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ....181-6T. § 1.181-1T;Deduction for qualified film and television production costs (temporary). (a.... (ii) Significantly incurred. (iii) Animated film and television productions. (iv) Productions..., and before March 12, 2007. (3) Information required. § 1.181-3TQualified film or television...

  13. 40 CFR 81.181 - Down East Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 17 2011-07-01 2011-07-01 false Down East Intrastate Air Quality Control Region. 81.181 Section 81.181 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Quality Control Regions § 81.181 Down East Intrastate Air Quality Control Region. The Down East...

  14. 40 CFR 81.181 - Down East Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Down East Intrastate Air Quality Control Region. 81.181 Section 81.181 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Quality Control Regions § 81.181 Down East Intrastate Air Quality Control Region. The Down East...

  15. 40 CFR 81.181 - Down East Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 18 2012-07-01 2012-07-01 false Down East Intrastate Air Quality Control Region. 81.181 Section 81.181 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Quality Control Regions § 81.181 Down East Intrastate Air Quality Control Region. The Down East...

  16. 40 CFR 81.181 - Down East Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 18 2014-07-01 2014-07-01 false Down East Intrastate Air Quality Control Region. 81.181 Section 81.181 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Quality Control Regions § 81.181 Down East Intrastate Air Quality Control Region. The Down East...

  17. 40 CFR 81.181 - Down East Intrastate Air Quality Control Region.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 18 2013-07-01 2013-07-01 false Down East Intrastate Air Quality Control Region. 81.181 Section 81.181 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... Quality Control Regions § 81.181 Down East Intrastate Air Quality Control Region. The Down East...

  18. 14 CFR 121.181 - Airplanes: Reciprocating engine-powered: En route limitations: One engine inoperative.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false Airplanes: Reciprocating engine-powered: En route limitations: One engine inoperative. 121.181 Section 121.181 Aeronautics and Space FEDERAL... OPERATIONS Airplane Performance Operating Limitations § 121.181 Airplanes: Reciprocating engine-powered:...

  19. 25 CFR 181.6 - How are applicants informed of the results?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 1 2013-04-01 2013-04-01 false How are applicants informed of the results? 181.6 Section 181.6 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAND AND WATER INDIAN HIGHWAY SAFETY PROGRAM § 181.6 How are applicants informed of the results? BIA will send a letter to...

  20. 25 CFR 181.6 - How are applicants informed of the results?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 1 2014-04-01 2014-04-01 false How are applicants informed of the results? 181.6 Section 181.6 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAND AND WATER INDIAN HIGHWAY SAFETY PROGRAM § 181.6 How are applicants informed of the results? BIA will send a letter to...

  1. 25 CFR 181.6 - How are applicants informed of the results?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false How are applicants informed of the results? 181.6 Section 181.6 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR LAND AND WATER INDIAN HIGHWAY SAFETY PROGRAM § 181.6 How are applicants informed of the results? BIA will send a letter to...

  2. 44 CFR 206.181 - Use of gifts and bequests for disaster assistance purposes.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... disaster assistance purposes. 206.181 Section 206.181 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY DISASTER ASSISTANCE FEDERAL DISASTER ASSISTANCE Other Individual Assistance § 206.181 Use of gifts and bequests for disaster assistance purposes. (a) General....

  3. 44 CFR 206.181 - Use of gifts and bequests for disaster assistance purposes.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... disaster assistance purposes. 206.181 Section 206.181 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY DISASTER ASSISTANCE FEDERAL DISASTER ASSISTANCE Other Individual Assistance § 206.181 Use of gifts and bequests for disaster assistance purposes. (a) General....

  4. 44 CFR 206.181 - Use of gifts and bequests for disaster assistance purposes.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... disaster assistance purposes. 206.181 Section 206.181 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY DISASTER ASSISTANCE FEDERAL DISASTER ASSISTANCE Other Individual Assistance § 206.181 Use of gifts and bequests for disaster assistance purposes. (a) General....

  5. 44 CFR 206.181 - Use of gifts and bequests for disaster assistance purposes.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... disaster assistance purposes. 206.181 Section 206.181 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY, DEPARTMENT OF HOMELAND SECURITY DISASTER ASSISTANCE FEDERAL DISASTER ASSISTANCE Other Individual Assistance § 206.181 Use of gifts and bequests for disaster assistance purposes. (a) General....

  6. 19 CFR 181.71 - Denial of preferential tariff treatment dependent on origin verification and determination.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... on origin verification and determination. 181.71 Section 181.71 Customs Duties U.S. CUSTOMS AND... FREE TRADE AGREEMENT Origin Verifications and Determinations § 181.71 Denial of preferential tariff treatment dependent on origin verification and determination. Except where a Certificate of Origin either...

  7. 19 CFR 181.51 - Prevention of improper payment of claims.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Prevention of improper payment of claims. 181.51 Section 181.51 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY...-Deferral Programs § 181.51 Prevention of improper payment of claims. (a) Double payment of claim....

  8. 36 CFR 18.1 - What is the authority and purpose for this part?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 36 Parks, Forests, and Public Property 1 2010-07-01 2010-07-01 false What is the authority and purpose for this part? 18.1 Section 18.1 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR LEASING OF PROPERTIES IN PARK AREAS § 18.1 What is the authority and purpose...

  9. 21 CFR 181.22 - Certain substances employed in the manufacture of food-packaging materials.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.22 Certain substances employed in the manufacture... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Certain substances employed in the manufacture of food-packaging materials. 181.22 Section 181.22 Food and Drugs FOOD AND DRUG ADMINISTRATION,...

  10. 21 CFR 181.22 - Certain substances employed in the manufacture of food-packaging materials.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.22 Certain substances employed in the manufacture... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Certain substances employed in the manufacture of food-packaging materials. 181.22 Section 181.22 Food and Drugs FOOD AND DRUG ADMINISTRATION,...

  11. 21 CFR 181.22 - Certain substances employed in the manufacture of food-packaging materials.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.22 Certain substances employed in the manufacture... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Certain substances employed in the manufacture of food-packaging materials. 181.22 Section 181.22 Food and Drugs FOOD AND DRUG ADMINISTRATION,...

  12. 26 CFR 1.181-0T - Table of contents (temporary).

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...-1T through 1.181-6T. § 1.181-1T;Deduction for qualified film and television production costs.... (i) In general. (ii) Significantly incurred. (iii) Animated film and television productions. (iv..., 2006, and before March 12, 2007. (3) Information required. § 1.181-3TQualified film or...

  13. Investigating the Impact of Asp181 Point Mutations on Interactions between PTP1B and Phosphotyrosine Substrate

    NASA Astrophysics Data System (ADS)

    Liu, Mengyuan; Wang, Lushan; Sun, Xun; Zhao, Xian

    2014-05-01

    Protein tyrosine phosphatase 1B (PTP1B) is a key negative regulator of insulin and leptin signaling, which suggests that it is an attractive therapeutic target in type II diabetes and obesity. The aim of this research is to explore residues which interact with phosphotyrosine substrate can be affected by D181 point mutations and lead to increased substrate binding. To achieve this goal, molecular dynamics simulations were performed on wild type (WT) and two mutated PTP1B/substrate complexes. The cross-correlation and principal component analyses show that point mutations can affect the motions of some residues in the active site of PTP1B. Moreover, the hydrogen bond and energy decomposition analyses indicate that apart from residue 181, point mutations have influence on the interactions of substrate with several residues in the active site of PTP1B.

  14. MicroRNA-181b promotes ovarian cancer cell growth and invasion by targeting LATS2

    SciTech Connect

    Xia, Ying; Gao, Yan

    2014-05-09

    Highlights: • miR-181b is upregulated in human ovarian cancer tissues. • miR-181b promotes ovarian cancer cell proliferation and invasion. • LATS2 is a direct target of miR-181b. • LATS2 is involved in miR-181b-induced ovarian cancer cell growth and invasion. - Abstract: MicroRNAs (miRNAs) are strongly implicated in tumorigenesis and metastasis. In this study, we showed significant upregulation of miR-181b in ovarian cancer tissues, compared with the normal ovarian counterparts. Forced expression of miR-181b led to remarkably enhanced proliferation and invasion of ovarian cancer cells while its knockdown induced significant suppression of these cellular events. The tumor suppressor gene, LATS2 (large tumor suppressor 2), was further identified as a novel direct target of miR-181b. Specifically, miR-181b bound directly to the 3′-untranslated region (UTR) of LATS2 and suppressed its expression. Restoration of LATS2 expression partially reversed the oncogenic effects of miR-181b. Our results indicate that miR-181b promotes proliferation and invasion by targeting LATS2 in ovarian cancer cells. These findings support the utility of miR-181b as a potential diagnostic and therapeutic target for ovarian cancer.

  15. Control of pT181 replication II. Mutational analysis.

    PubMed Central

    Carleton, S; Projan, S J; Highlander, S K; Moghazeh, S M; Novick, R P

    1984-01-01

    We describe the isolation and analysis of mutations affecting the regulation of Staphylococcus aureus plasmid pT181 replication. Previous results suggested that regulation is achieved by control of the synthesis of RepC, a plasmid-coded replication protein and that the primary negative control element is CopA RNA, which consists of two transcripts that are complementary to the 5' region of the repC mRNA leader. CopA inhibition probably involves a base pairing interaction with the complementary region of the RepC mRNA leader which would facilitate the formation of a downstream stem-loop in the leader that occludes the repC ribosome binding site. RepC is freely diffusible so that regulation of pT181 replication is indirect. Both CopA RNA-sensitive (recessive) and -insensitive (dominant) mutants were isolated. The recessives have defects in CopA RNA structure or activity, the dominants have defects in the site of action (target) of the inhibitor. Some dominants were located within the copA coding sequence. These therefore affect the structure of CopA RNA as well as that of its target. Other dominant mutations mapped outside of the copA gene and therefore produced wild-type CopA RNA. In contrast to directly regulated plasmids, pT181 copy mutants producing wild-type inhibitor could be co-maintained with the wild-type plasmid and mutational changes in inhibitor-target specificity did not change incompatibility specificity. Images Fig. 1. Fig. 4. PMID:6437809

  16. microRNA-181a has a critical role in ovarian cancer progression through the regulation of the epithelial-mesenchymal transition

    NASA Astrophysics Data System (ADS)

    Parikh, Aditya; Lee, Christine; Joseph, Peronne; Marchini, Sergio; Baccarini, Alessia; Kolev, Valentin; Romualdi, Chiara; Fruscio, Robert; Shah, Hardik; Wang, Feng; Mullokandov, Gavriel; Fishman, David; D'Incalci, Maurizio; Rahaman, Jamal; Kalir, Tamara; Redline, Raymond W.; Brown, Brian D.; Narla, Goutham; Difeo, Analisa

    2014-01-01

    Ovarian cancer is a leading cause of cancer deaths among women. Effective targets to treat advanced epithelial ovarian cancer (EOC) and biomarkers to predict treatment response are still lacking because of the complexity of pathways involved in ovarian cancer progression. Here we show that miR-181a promotes TGF-β-mediated epithelial-to-mesenchymal transition via repression of its functional target, Smad7. miR-181a and phosphorylated Smad2 are enriched in recurrent compared with matched-primary ovarian tumours and their expression is associated with shorter time to recurrence and poor outcome in patients with EOC. Furthermore, ectopic expression of miR-181a results in increased cellular survival, migration, invasion, drug resistance and in vivo tumour burden and dissemination. In contrast, miR-181a inhibition via decoy vector suppression and Smad7 re-expression results in significant reversion of these phenotypes. Combined, our findings highlight an unappreciated role for miR-181a, Smad7, and the TGF-β signalling pathway in high-grade serous ovarian cancer.

  17. Silencing miR-181a produces neuroprotection against hippocampus neuron cell apoptosis post-status epilepticus in a rat model and in children with temporal lobe epilepsy.

    PubMed

    Ren, L; Zhu, R; Li, X

    2016-01-01

    Epilepsy is one of the most frequent neurological disorders. Recently, the regulation of microRNAs was found to be associated with epilepsy, but the molecular mechanism by which microRNA influences epilepsy process remains to be unveiled and the development of microRNA-based therapy requires more intensive research. In this study, five microRNAs with potential relevance to epilepsy were initially chosen: miR-132, miR-146a, miR-181a, miR-34a, and miR-124. Twenty-five children who were patients with epilepsy were selected as subjects to obtain tissue samples for the study. The miRNA-181a, which represented the most increased fold-changes in clinical samples, were then selected for further function study in mouse model. The temporal lobe epilepsy (TLE) model, along with lithium-pilocarpine-induced status epilepticus (SE), was established in Sprague-Dawley rats. The antagomir of miR-181a was used to determine the role of miR-181a in cell apoptosis. Analyses were conducted to determine the expression levels of miR-181a, neuronal apoptosis in post-SE, and activated caspase-3. We found evidence of significant time dependent up-regulation of miR-181a amongst post-SE rats and TLE on 24 h (4.47 ± 0.35), 7 days (4.85 ± 0.53), and 2 weeks (5.66 ± 0.64). Experiments with the miR-181a antagomir showed that this particular miRNA led to the inhibition of the protein expression of caspase-3, and was up-regulated in the course of seizure-induced neuronal apoptosis. This study provided evidence that targeting miR-181a leads to a neuroprotective response and is linked to an increase in the activation of the caspase-3 protein. These findings suggest that miR-181a may serve as a promising therapeutic target for epilepsy. PMID:26910006

  18. Upregulation of miR-181c contributes to chemoresistance in pancreatic cancer by inactivating the Hippo signaling pathway

    PubMed Central

    Guo, Xingjun; Jiang, Jianxin

    2015-01-01

    The Hippo signaling pathway plays a crucial role in regulating tissue homeostasis, organ size, tumorigenesis and cancer chemoresistance when deregulated. Physiologically, the Hippo core kinase cassette that consists of mamma-lian STE20-like protein kinase 1/2 (MST1/2), and large tumour suppressor 1/2 (LATS1/2), together with the adaptor proteins Salvador homologue 1 (SAV1) and MOB kinase activator 1 (MOB1), tightly restricts the activities of homologous oncoproteins Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) to low levels. However, how the Hippo kinase cassette core components are simultaneously inhibited, to exhibit constitutively inactivated Hippo signaling and activated YAP/TAZ in cancer remains puzzling. Herein, we reported that miR-181c directly repressed MST1, LATS2, MOB1 and SAV1 expression in human pancreatic cancer cells. Overexpression of miR-181c induced hyperactivation of the YAP/TAZ and enhanced expression of the Hippo signaling downstream genes CTGF, BIRC5 and BLC2L1, leading to pancreatic cancer cell survival and chemoresistance in vitro and in vivo. Importantly, high miR-181c levels were significantly correlated with Hippo signaling inactivation in pancreatic cancer samples, and predicted a poor patient overall survival. These findings provide a novel mechanism for Hippo signaling inactivation in cancer, indicating not only a potentially pivotal role for miR-181c in the progression of pancreatic cancer, but also may represent a new therapeutic target and prognostic marker. PMID:26561204

  19. Polycyclic aromatic hydrocarbon (PAH)-mediated upregulation of hepatic microRNA-181 family promotes cancer cell migration by targeting MAPK phosphatase-5, regulating the activation of p38 MAPK

    SciTech Connect

    Song, Mi-Kyung; Park, Yong-Keun; Ryu, Jae-Chun

    2013-11-15

    Growing evidence indicates that changes in microRNA (miRNA) expression in cancer induced by chemical carcinogens play an important role in cancer development and progression by regulating related genes. However, the mechanisms underlying miRNA involvement in hepatocarcinogenesis induced by polycyclic aromatic hydrocarbons (PAHs) remain unclear. Thus, the identification of aberrant miRNA expression during PAH-induced cancer cell migration will lead to a better understanding of the substantial role of miRNAs in cancer progression. In the present study, miRNA expression profiling showed significant upregulation of miR-181a, -181b, and -181d in human hepatocellular carcinoma cells (HepG2 line) exposed to benzo[a]anthracene (BA) and benzo[k]fluoranthene (BF). MAPK phosphatase-5 (MKP-5), a validated miR-181 target that deactivates MAPKs, was markedly suppressed while phosphorylation of p38 MAPK was increased after BA and BF exposure. The migration of HepG2 cells, observed using the scratch wound-healing assay, also increased in a dose-dependent manner. Depletion of miR-181 family members by miRNA inhibitors enhanced the expression of MKP-5 and suppressed the phosphorylation of p38 MAPK. Furthermore, the depletion of the miR-181 family inhibited cancer cell migration. Based on these results, we conclude that the miR-181 family plays a critical role in PAH-induced hepatocarcinogenesis by targeting MKP-5, resulting in the regulation of p38 MAPK activation. - Highlights: • We found significant upregulation of miR-181 family in HCC exposed to BA and BF. • We identified the MKP-5 as a putative target of miR-181 family. • MKP-5 was suppressed while p-P38 was increased after BA and BF exposure. • The migration of HepG2 cells increased in a dose-dependent manner.

  20. Cross section of reaction 181Ta(p,nγ) 181W and the influence of the spin cut-off parameter on the cross section

    NASA Astrophysics Data System (ADS)

    Zhang, Jun-Jie; Hu, Bi-Tao

    2011-12-01

    In this work, the program Cindy was modified to calculate the formation cross section of each energy level of residual nucleus 181W resulting from the reaction 181Ta(p,nγ) 181W. The concerned cross sections calculated at proton energy Ep = 4.5-8.5 MeV agreed well with experimental results. The influence of the spin cut-off parameter in the energy level density model on the cross section was studied. The obtained results show that the influence of spin cut-off is obvious for lower energy levels.

  1. Time-resolved photointermediate changes in rhodopsin glutamic acid 181 mutants.

    PubMed

    Lewis, James W; Szundi, Istvan; Kazmi, Manija A; Sakmar, Thomas P; Kliger, David S

    2004-10-01

    The role of glutamic acid 181 in the bovine rhodopsin retinylidene chromophore pocket was studied by expressing E181 mutants in COS cells and measuring, as a function of time, the absorbance changes produced after excitation of lauryl maltoside pigment suspensions with 7 ns laser pulses. All mutants studied except E181D showed accelerated decay of bathorhodopsin compared to wild type. Even for E181D, an anomalously large blue shift was observed in the absorption spectrum of the bathorhodopsin decay product, BSI. These observations support the idea that E181 plays a significant role in the earliest stages of receptor activation. E181 mutations have a pronounced effect on the decay of the lumirhodopsin photointermediate, primarily affecting the size of the red shift that occurs in the lumirhodopsin I to lumirhodopsin II transition that takes place on the 10 micros time scale after wild-type photoexcitation. While the spectral change that occurs in the lumirhodopsin I to lumirhodopsin II transition in wild-type rhodopsin is very small ( approximately 2 nm), making it difficult to detect, it is larger in E181D ( approximately 6 nm), making it evident even in the lower signal-to-noise ratio measurements possible with rhodopsin mutants. The change seen is even larger for the E181F mutant where significant amounts of a deprotonated Schiff base intermediate are produced with the 10 micros time constant of lumirhodopsin II formation. The E181Q mutant shows lumirhodopsin decay more similar to wild-type behavior, and no lumirhodopsin I to lumirhodopsin II transition can be resolved. The addition of chloride ion to E181Q increases the lumirhodopsin I-lumirhodopsin II spectral shift and slows the deprotonation of the Schiff base. The latter result is consistent with the idea that a negative charge at position 181 contributes to protonated Schiff base stability in the later intermediates.

  2. Signatures of miR-181a on the Renal Transcriptome and Blood Pressure

    PubMed Central

    Marques, Francine Z; Romaine, Simon PR; Denniff, Matthew; Eales, James; Dormer, John; Garrelds, Ingrid M; Wojnar, Lukasz; Musialik, Katarzyna; Duda-Raszewska, Barbara; Kiszka, Bartlomiej; Duda, Magdalena; Morris, Brian J; Samani, Nilesh J; Danser, AH Jan; Bogdanski, Pawel; Zukowska-Szczechowska, Ewa; Charchar, Fadi J; Tomaszewski, Maciej

    2015-01-01

    MicroRNA-181a binds to the 3′ untranslated region of messenger RNA (mRNA) for renin, a rate-limiting enzyme of the renin-angiotensin system. Our objective was to determine whether this molecular interaction translates into a clinically meaningful effect on blood pressure and whether circulating miR-181a is a measurable proxy of blood pressure. In 200 human kidneys from the TRANScriptome of renaL humAn TissuE (TRANSLATE) study, renal miR-181a was the sole negative predictor of renin mRNA and a strong correlate of circulating miR-181a. Elevated miR-181a levels correlated positively with systolic and diastolic blood pressure in TRANSLATE, and this association was independent of circulating renin. The association between serum miR-181a and systolic blood pressure was replicated in 199 subjects from the Genetic Regulation of Arterial Pressure of Humans In the Community (GRAPHIC) study. Renal immunohistochemistry and in situ hybridization showed that colocalization of miR-181a and renin was most prominent in collecting ducts where renin is not released into the systemic circulation. Analysis of 69 human kidneys characterized by RNA sequencing revealed that miR-181a was associated with downregulation of four mitochondrial pathways and upregulation of 41 signaling cascades of adaptive immunity and inflammation. We conclude that renal miR-181a has pleiotropic effects on pathways relevant to blood pressure regulation and that circulating levels of miR-181a are both a measurable proxy of renal miR-181a expression and a novel biochemical correlate of blood pressure. PMID:26322847

  3. Where Will LEAD Lead?

    ERIC Educational Resources Information Center

    Wildman, Louis

    After setting forth eight assumptions concerning the education of educational administrators, findings about the Leadership in Educational Administration Development (LEAD) program are discussed. The analysis is based on the first-year applications, telephone conversations with staff at a majority of the project sites, and additional material…

  4. 46 CFR 69.181 - Locating the line of the second deck.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 2 2014-10-01 2014-10-01 false Locating the line of the second deck. 69.181 Section 69... VESSELS MEASUREMENT OF VESSELS Dual Measurement System § 69.181 Locating the line of the second deck. (a) If the second deck is not stepped, the line of the second deck is the longitudinal line of...

  5. 46 CFR 69.181 - Locating the line of the second deck.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 2 2012-10-01 2012-10-01 false Locating the line of the second deck. 69.181 Section 69... VESSELS MEASUREMENT OF VESSELS Dual Measurement System § 69.181 Locating the line of the second deck. (a) If the second deck is not stepped, the line of the second deck is the longitudinal line of...

  6. 46 CFR 69.181 - Locating the line of the second deck.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 2 2013-10-01 2013-10-01 false Locating the line of the second deck. 69.181 Section 69... VESSELS MEASUREMENT OF VESSELS Dual Measurement System § 69.181 Locating the line of the second deck. (a) If the second deck is not stepped, the line of the second deck is the longitudinal line of...

  7. 46 CFR 69.181 - Locating the line of the second deck.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 2 2011-10-01 2011-10-01 false Locating the line of the second deck. 69.181 Section 69... VESSELS MEASUREMENT OF VESSELS Dual Measurement System § 69.181 Locating the line of the second deck. (a) If the second deck is not stepped, the line of the second deck is the longitudinal line of...

  8. 26 CFR 1.181-6T - Effective/applicability dates (temporary).

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... paragraph (b) of this section, § 1.181-1T applies to productions, the first day of principal photography for... animation” in place of “principal photography.” Productions involving both animation and live-action photography may use either standard. (2) The applicability of § 1.181-1T expires on October 17, 2014....

  9. 19 CFR 181.99 - Issuance of NAFTA advance rulings or other advice.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Issuance of NAFTA advance rulings or other advice. 181.99 Section 181.99 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND... advance ruling letter in the English language setting forth the position of Customs and the...

  10. 22 CFR 181.5 - Twenty-day rule for concluded agreements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Section 181.5 Foreign Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.5 Twenty-day rule for concluded agreements. (a) Any agency, including the Department of State, that concludes an international agreement within the meaning of the...

  11. 21 CFR 181.26 - Drying oils as components of finished resins.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...) shall include: Chinawood oil (tung oil). Dehydrated castor oil. Linseed oil. Tall oil. ... 21 Food and Drugs 3 2011-04-01 2011-04-01 false Drying oils as components of finished resins. 181... Prior-Sanctioned Food Ingredients § 181.26 Drying oils as components of finished resins....

  12. 19 CFR 181.22 - Maintenance of records and submission of Certificate by importer.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Maintenance of records and submission of Certificate by importer. 181.22 Section 181.22 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE...

  13. 19 CFR 181.22 - Maintenance of records and submission of Certificate by importer.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Maintenance of records and submission of Certificate by importer. 181.22 Section 181.22 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE...

  14. 42 CFR 456.181 - Reports of evaluations and plans of care.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 4 2012-10-01 2012-10-01 false Reports of evaluations and plans of care. 456.181 Section 456.181 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL ASSISTANCE PROGRAMS UTILIZATION CONTROL Utilization Control: Mental...

  15. 42 CFR 456.181 - Reports of evaluations and plans of care.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 4 2014-10-01 2014-10-01 false Reports of evaluations and plans of care. 456.181 Section 456.181 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL ASSISTANCE PROGRAMS UTILIZATION CONTROL Utilization Control: Mental...

  16. 42 CFR 456.181 - Reports of evaluations and plans of care.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Reports of evaluations and plans of care. 456.181 Section 456.181 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL ASSISTANCE PROGRAMS UTILIZATION CONTROL Utilization Control: Mental...

  17. 42 CFR 456.181 - Reports of evaluations and plans of care.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 4 2011-10-01 2011-10-01 false Reports of evaluations and plans of care. 456.181 Section 456.181 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL ASSISTANCE PROGRAMS UTILIZATION CONTROL Utilization Control: Mental...

  18. 42 CFR 456.181 - Reports of evaluations and plans of care.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 4 2013-10-01 2013-10-01 false Reports of evaluations and plans of care. 456.181 Section 456.181 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL ASSISTANCE PROGRAMS UTILIZATION CONTROL Utilization Control: Mental...

  19. 20 CFR 1002.181 - How is “prompt reemployment” defined?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 4 2012-04-01 2012-04-01 false How is âprompt reemploymentâ defined? 1002.181 Section 1002.181 Employees' Benefits OFFICE OF THE ASSISTANT SECRETARY FOR VETERANS' EMPLOYMENT... regularly scheduled working day. On the other hand, prompt reinstatement following several years of...

  20. 43 CFR 30.181 - How do I renounce an inherited interest?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... declaration, you may retain a life estate in a specified interest in trust or restricted land and renounce the... 43 Public Lands: Interior 1 2013-10-01 2013-10-01 false How do I renounce an inherited interest? 30.181 Section 30.181 Public Lands: Interior Office of the Secretary of the Interior INDIAN...

  1. 43 CFR 30.181 - How do I renounce an inherited interest?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... declaration, you may retain a life estate in a specified interest in trust or restricted land and renounce the... 43 Public Lands: Interior 1 2014-10-01 2014-10-01 false How do I renounce an inherited interest? 30.181 Section 30.181 Public Lands: Interior Office of the Secretary of the Interior INDIAN...

  2. 43 CFR 30.181 - How do I renounce an inherited interest?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... declaration, you may retain a life estate in a specified interest in trust or restricted land and renounce the... 43 Public Lands: Interior 1 2012-10-01 2011-10-01 true How do I renounce an inherited interest? 30.181 Section 30.181 Public Lands: Interior Office of the Secretary of the Interior INDIAN...

  3. 26 CFR 1.181-1T - Deduction for qualified film and television production costs (temporary).

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 26 Internal Revenue 3 2012-04-01 2012-04-01 false Deduction for qualified film and television... Individuals and Corporations (continued) § 1.181-1T Deduction for qualified film and television production... concerning qualified film and television productions. Section 1.181-4 provides special rules, including...

  4. 33 CFR 181.704 - Contents of information pamphlet: Recreational hybrid PFD.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... pamphlet for a recreational hybrid PFD approved under 46 CFR 160.077 must contain the information specified in 46 CFR 160.077-27. ...: Recreational hybrid PFD. 181.704 Section 181.704 Navigation and Navigable Waters COAST GUARD, DEPARTMENT...

  5. 33 CFR 181.704 - Contents of information pamphlet: Recreational hybrid PFD.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... pamphlet for a recreational hybrid PFD approved under 46 CFR 160.077 must contain the information specified in 46 CFR 160.077-27. ...: Recreational hybrid PFD. 181.704 Section 181.704 Navigation and Navigable Waters COAST GUARD, DEPARTMENT...

  6. 33 CFR 181.704 - Contents of information pamphlet: Recreational hybrid PFD.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... pamphlet for a recreational hybrid PFD approved under 46 CFR 160.077 must contain the information specified in 46 CFR 160.077-27. ...: Recreational hybrid PFD. 181.704 Section 181.704 Navigation and Navigable Waters COAST GUARD, DEPARTMENT...

  7. 33 CFR 181.704 - Contents of information pamphlet: Recreational hybrid PFD.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... pamphlet for a recreational hybrid PFD approved under 46 CFR 160.077 must contain the information specified in 46 CFR 160.077-27. ...: Recreational hybrid PFD. 181.704 Section 181.704 Navigation and Navigable Waters COAST GUARD, DEPARTMENT...

  8. 33 CFR 181.704 - Contents of information pamphlet: Recreational hybrid PFD.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... pamphlet for a recreational hybrid PFD approved under 46 CFR 160.077 must contain the information specified in 46 CFR 160.077-27. ...: Recreational hybrid PFD. 181.704 Section 181.704 Navigation and Navigable Waters COAST GUARD, DEPARTMENT...

  9. 21 CFR 181.26 - Drying oils as components of finished resins.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 3 2010-04-01 2009-04-01 true Drying oils as components of finished resins. 181... Prior-Sanctioned Food Ingredients § 181.26 Drying oils as components of finished resins. Substances classified as drying oils, when migrating from food-packaging material (as components of finished...

  10. 21 CFR 181.26 - Drying oils as components of finished resins.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Drying oils as components of finished resins. 181.26 Section 181.26 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients §...

  11. MIR181A regulates starvation- and rapamycin-induced autophagy through targeting of ATG5

    PubMed Central

    Tekirdag, Kumsal Ayse; Korkmaz, Gozde; Ozturk, Deniz Gulfem; Agami, Reuven; Gozuacik, Devrim

    2013-01-01

    Macroautophagy (autophagy herein) is a cellular catabolic mechanism activated in response to stress conditions including starvation, hypoxia and misfolded protein accumulation. Abnormalities in autophagy were associated with pathologies including cancer and neurodegenerative diseases. Hence, elucidation of the signaling pathways controlling autophagy is of utmost importance. Recently we and others described microRNAs (miRNAs) as novel and potent modulators of the autophagic activity. Here, we describe MIR181A (hsa-miR-181a-1) as a new autophagy-regulating miRNA. We showed that overexpression of MIR181A resulted in the attenuation of starvation- and rapamycin-induced autophagy in MCF-7, Huh-7 and K562 cells. Moreover, antagomir-mediated inactivation of endogenous miRNA activity stimulated autophagy. We identified ATG5 as an MIR181A target. Indeed, ATG5 cellular levels were decreased in cells upon MIR181A overexpression and increased following the introduction of antagomirs. More importantly, overexpression of ATG5 from a miRNA-insensitive cDNA construct rescued autophagic activity in the presence of MIR181A. We also showed that the ATG5 3′ UTR contained functional MIR181A responsive sequences sensitive to point mutations. Therefore, MIR181A is a novel and important regulator of autophagy and ATG5 is a rate-limiting miRNA target in this effect. PMID:23322078

  12. 22 CFR 181.5 - Twenty-day rule for concluded agreements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Section 181.5 Foreign Relations DEPARTMENT OF STATE INTERNATIONAL AGREEMENTS COORDINATION, REPORTING AND PUBLICATION OF INTERNATIONAL AGREEMENTS § 181.5 Twenty-day rule for concluded agreements. (a) Any agency, including the Department of State, that concludes an international agreement within the meaning of the...

  13. Munc18-1 mutations that strongly impair SNARE-complex binding support normal synaptic transmission

    PubMed Central

    Meijer, Marieke; Burkhardt, Pawel; de Wit, Heidi; Toonen, Ruud F; Fasshauer, Dirk; Verhage, Matthijs

    2012-01-01

    Synaptic transmission depends critically on the Sec1p/Munc18 protein Munc18-1, but it is unclear whether Munc18-1 primarily operates as a integral part of the fusion machinery or has a more upstream role in fusion complex assembly. Here, we show that point mutations in Munc18-1 that interfere with binding to the free Syntaxin1a N-terminus and strongly impair binding to assembled SNARE complexes all support normal docking, priming and fusion of synaptic vesicles, and normal synaptic plasticity in munc18-1 null mutant neurons. These data support a prevailing role of Munc18-1 before/during SNARE-complex assembly, while its continued association to assembled SNARE complexes is dispensable for synaptic transmission. PMID:22446389

  14. LEADING WITH LEADING INDICATORS

    SciTech Connect

    PREVETTE, S.S.

    2005-01-27

    This paper documents Fluor Hanford's use of Leading Indicators, management leadership, and statistical methodology in order to improve safe performance of work. By applying these methods, Fluor Hanford achieved a significant reduction in injury rates in 2003 and 2004, and the improvement continues today. The integration of data, leadership, and teamwork pays off with improved safety performance and credibility with the customer. The use of Statistical Process Control, Pareto Charts, and Systems Thinking and their effect on management decisions and employee involvement are discussed. Included are practical examples of choosing leading indicators. A statistically based color coded dashboard presentation system methodology is provided. These tools, management theories and methods, coupled with involved leadership and employee efforts, directly led to significant improvements in worker safety and health, and environmental protection and restoration at one of the nation's largest nuclear cleanup sites.

  15. MicroRNA-181b regulates NF-κB–mediated vascular inflammation

    PubMed Central

    Sun, Xinghui; Icli, Basak; Wara, Akm Khyrul; Belkin, Nathan; He, Shaolin; Kobzik, Lester; Hunninghake, Gary M.; Vera, Miguel Pinilla; Blackwell, Timothy S.; Baron, Rebecca M.; Feinberg, Mark W.

    2012-01-01

    EC activation and dysfunction have been linked to a variety of vascular inflammatory disease states. The function of microRNAs (miRNAs) in vascular EC activation and inflammation remains poorly understood. Herein, we report that microRNA-181b (miR-181b) serves as a potent regulator of downstream NF-κB signaling in the vascular endothelium by targeting importin-α3, a protein that is required for nuclear translocation of NF-κB. Overexpression of miR-181b inhibited importin-α3 expression and an enriched set of NF-κB–responsive genes such as adhesion molecules VCAM-1 and E-selectin in ECs in vitro and in vivo. In addition, treatment of mice with proinflammatory stimuli reduced miR-181b expression. Rescue of miR-181b levels by systemic administration of miR-181b “mimics” reduced downstream NF-κB signaling and leukocyte influx in the vascular endothelium and decreased lung injury and mortality in endotoxemic mice. In contrast, miR-181b inhibition exacerbated endotoxin-induced NF-κB activity, leukocyte influx, and lung injury. Finally, we observed that critically ill patients with sepsis had reduced levels of miR-181b compared with control intensive care unit (ICU) subjects. Collectively, these findings demonstrate that miR-181b regulates NF-κB–mediated EC activation and vascular inflammation in response to proinflammatory stimuli and that rescue of miR-181b expression could provide a new target for antiinflammatory therapy and critical illness. PMID:22622040

  16. miR-181a shows tumor suppressive effect against oral squamous cell carcinoma cells by downregulating K-ras

    SciTech Connect

    Shin, Ki-Hyuk; Bae, Susan D.; Hong, Hannah S.; Kim, Reuben H.; Kang, Mo K.; Park, No-Hee

    2011-01-28

    Research highlights: {yields} MicroRNA-181a (miR-181a) was frequently downregulated in oral squamous cell carcinoma (OSCC). {yields} Overexpression of miR-181a suppressed OSCC growth. {yields} K-ras is a novel target of miR-181a. {yields} Decreased miR-181a expression is attributed to its lower promoter activity in OSCC. -- Abstract: MicroRNAs (miRNAs) are epigenetic regulators of gene expression, and their deregulation plays an important role in human cancer, including oral squamous cell carcinoma (OSCC). Recently, we found that miRNA-181a (miR-181a) was upregulated during replicative senescence of normal human oral keratinocytes. Since senescence is considered as a tumor suppressive mechanism, we thus investigated the expression and biological role of miR-181a in OSCC. We found that miR-181a was frequently downregulated in OSCC. Ectopic expression of miR-181a suppressed proliferation and anchorage independent growth ability of OSCC. Moreover, miR-181a dramatically reduces the growth of OSCC on three dimensional organotypic raft culture. We also identified K-ras as a novel target of miR-181a. miR-181a decreased K-ras protein level as well as the luciferase activity of reporter vectors containing the 3'-untranslated region of K-ras gene. Finally, we defined a minimal regulatory region of miR-181a and found a positive correlation between its promoter activity and the level of miR-181a expression. In conclusion, miR-181a may function as an OSCC suppressor by targeting on K-ras oncogene. Thus, miR-181a should be considered for therapeutic application for OSCC.

  17. MiR-181a influences the cognitive function of epileptic rats induced by pentylenetetrazol

    PubMed Central

    Huang, Yiqing; Liu, Xixia; Liao, Yuhan; Luo, Chun; Zou, Donghua; Wei, Xing; Huang, Qi; Wu, Yuan

    2015-01-01

    Our previous study showed that the expression of miR-181a in memory impairment group of pentylenetetrazol (PTZ)-induced epileptic rats was up-regulated, but whether miR-181a influenced the cognitive function of PTZ-induced epileptic rats remains unknown. Therefore, we investigated the role of miR-181a in the cognitive function of PTZ-induced epileptic rats. A model of temporal lobe epilepsy (TLE) was induced via PTZ kindling in SD male rats. The epileptic rats were divided into Epilepsy group, Agomir-control group, miR-181a agomir group, 12 rats for each. 12 rats were used as sham group. We found that compared to the sham group, the expression of miR-181a in the Epilepsy group was increased. We also found that escape latency in the 5th day was prolonged and crossing times in the 6th day was reduced via Morris Water Maze test, which may indicate memory impairment. Furthermore, over-expression of miR-181a effectively reduced Bcl-2 protein level and increased apoptosis in hippocampus. Moreover, compared with Agomir-control group, the escape latency of miR-181a agomir group was obviously induced (P<0.05). Our findings suggest that miR-181a may play a role in impairing the cognitive function of PTZ-induced epileptic rats, and miR-181a could decrease the Bcl-2 protein and induce the apoptosis in the hippocampus that might be the way to impair cognitive function. PMID:26722477

  18. Deregulation of miRNA-181c potentially contributes to the pathogenesis of AD by targeting collapsin response mediator protein 2 in mice.

    PubMed

    Zhou, Huimin; Zhang, Rui; Lu, Kang; Yu, Wenjun; Xie, Bing; Cui, Dongsheng; Jiang, Lei; Zhang, Qingfu; Xu, Shunjiang

    2016-08-15

    Alzheimer's disease (AD) is a progressive neurodegenerative disorder that is usually accompanied by abnormal gene expression. The 20 to 25 nucleotide (nt) tiny regulators, known as micro ribonucleic acids (miRNAs), have been found to play important roles in the etiology and pathogenesis of various biological processes. The purpose of the current study was to identify the aberrant expression of microRNAs in the hippocampus of an AD mouse model and to investigate its potential role during the progression of AD. The results from microarray analysis showed that several miRNAs were deregulated in the hippocampus tissue of SAMP8 mice compared to SAMR1 mice. Among the deregulated miRNAs, a significant decrease in miR-181c was validated by quantitative real-time PCR. Bioinformatic analysis revealed that miR-181c might be involved in the regulation of axon guidance, MAPK signaling, dorso-ventral axis formation and long-term depression. Moreover, the results of a luciferase activity assay, western blot analysis and immunofluorescent staining showed that over-expression of miR-181c targets the 3'-untranslated region (3'-UTR) of collapsin response mediator protein 2 (crmp2) through its binding sites and down-regulates crmp2 protein abundance at the post-transcriptional level. Taken together, these findings suggested that crmp2 is a target of miR-181c and that the abnormally low expression of miR-181c in the hippocampus of SAMP8 mice could lead to an increase of the crmp2 protein level in AD mice, which might potentially play a role in the pathogenesis of Alzheimer's disease. PMID:27423553

  19. Low-Temperature Trapping of Photointermediates of the Rhodopsin E181Q Mutant

    PubMed Central

    Sandberg, Megan N.; Greco, Jordan A.; Wagner, Nicole L.; Amora, Tabitha L.; Ramos, Lavoisier A.; Chen, Min-Hsuan; Knox, Barry E.; Birge, Robert R.

    2015-01-01

    Three active-site components in rhodopsin play a key role in the stability and function of the protein: 1) the counter-ion residues which stabilize the protonated Schiff base, 2) water molecules, and 3) the hydrogen-bonding network. The ionizable residue Glu-181, which is involved in an extended hydrogen-bonding network with Ser-186, Tyr-268, Tyr-192, and key water molecules within the active site of rhodopsin, has been shown to be involved in a complex counter-ion switch mechanism with Glu-113 during the photobleaching sequence of the protein. Herein, we examine the photobleaching sequence of the E181Q rhodopsin mutant by using cryogenic UV-visible spectroscopy to further elucidate the role of Glu-181 during photoactivation of the protein. We find that lower temperatures are required to trap the early photostationary states of the E181Q mutant compared to native rhodopsin. Additionally, a Blue Shifted Intermediate (BSI, λmax = 498 nm, 100 K) is observed after the formation of E181Q Bathorhodopsin (Batho, λmax = 556 nm, 10 K) but prior to formation of E181Q Lumirhodopsin (Lumi, λmax = 506 nm, 220 K). A potential energy diagram of the observed photointermediates suggests the E181Q Batho intermediate has an enthalpy value 7.99 KJ/mol higher than E181Q BSI, whereas in rhodopsin, the BSI is 10.02 KJ/mol higher in enthalpy than Batho. Thus, the Batho to BSI transition is enthalpically driven in E181Q and entropically driven in native rhodopsin. We conclude that the substitution of Glu-181 with Gln-181 results in a significant perturbation of the hydrogen-bonding network within the active site of rhodopsin. In addition, the removal of a key electrostatic interaction between the chromophore and the protein destabilizes the protein in both the dark state and Batho intermediate conformations while having a stabilizing effect on the BSI conformation. The observed destabilization upon this substitution further supports that Glu-181 is negatively charged in the early

  20. 34 CFR 403.181 - What are the cost-sharing requirements applicable to the basic programs?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... APPLIED TECHNOLOGY EDUCATION PROGRAM What Financial Conditions Must Be Met by a State? § 403.181 What are... 34 Education 3 2011-07-01 2011-07-01 false What are the cost-sharing requirements applicable to the basic programs? 403.181 Section 403.181 Education Regulations of the Offices of the Department...

  1. 42 CFR 440.181 - Home and community-based services for individuals age 65 or older.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Definitions § 440.181 Home and community-based services for individuals age 65 or older. (a) Description of services— Home and community-based services for individuals age 65 or older means services, not otherwise... age 65 or older. 440.181 Section 440.181 Public Health CENTERS FOR MEDICARE & MEDICAID...

  2. 42 CFR 440.181 - Home and community-based services for individuals age 65 or older.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Definitions § 440.181 Home and community-based services for individuals age 65 or older. (a) Description of services— Home and community-based services for individuals age 65 or older means services, not otherwise... age 65 or older. 440.181 Section 440.181 Public Health CENTERS FOR MEDICARE & MEDICAID...

  3. 42 CFR 440.181 - Home and community-based services for individuals age 65 or older.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Definitions § 440.181 Home and community-based services for individuals age 65 or older. (a) Description of services— Home and community-based services for individuals age 65 or older means services, not otherwise... age 65 or older. 440.181 Section 440.181 Public Health CENTERS FOR MEDICARE & MEDICAID...

  4. 42 CFR 440.181 - Home and community-based services for individuals age 65 or older.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Definitions § 440.181 Home and community-based services for individuals age 65 or older. (a) Description of services— Home and community-based services for individuals age 65 or older means services, not otherwise... age 65 or older. 440.181 Section 440.181 Public Health CENTERS FOR MEDICARE & MEDICAID...

  5. 42 CFR 440.181 - Home and community-based services for individuals age 65 or older.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... age 65 or older. 440.181 Section 440.181 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES... Definitions § 440.181 Home and community-based services for individuals age 65 or older. (a) Description of services— Home and community-based services for individuals age 65 or older means services, not...

  6. 34 CFR 403.181 - What are the cost-sharing requirements applicable to the basic programs?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... APPLIED TECHNOLOGY EDUCATION PROGRAM What Financial Conditions Must Be Met by a State? § 403.181 What are... 34 Education 3 2010-07-01 2010-07-01 false What are the cost-sharing requirements applicable to the basic programs? 403.181 Section 403.181 Education Regulations of the Offices of the Department...

  7. Lead Poisoning

    MedlinePlus

    ... Experiments Stories Lessons Topics Games Activities Lessons MENU Lead Poisoning Kids Homepage Topics Pollution Lead Poisoning What is ... you can avoid contact with it! Sources of Lead Poisoning HOUSE PAINTS: Before1950, lead-based paint was used ...

  8. Lead Test

    MedlinePlus

    ... to determine lead sources, educating family members about lead poisoning , and instituting follow-up testing to monitor the ... high levels of lead, see the article on Lead Poisoning . The Occupational Safety and Health Administration (OSHA) has ...

  9. Lead Poisoning

    MedlinePlus

    Lead Poisoning What is it and who is affected? Lead is a highly toxic substance, exposure to which ... and children can suffer from the effects of lead poisoning, but childhood lead poisoning is much more frequent. ...

  10. Pre-miRNA Loop Nucleotides Control the Distinct Activities of mir-181a-1 and mir-181c in Early T Cell Development

    PubMed Central

    Yue, Sibiao; Chen, Chang-Zheng

    2008-01-01

    Background Mature miRNAs can often be classified into large families, consisting of members with identical seeds (nucleotides 2 through 7 of the mature miRNAs) and highly homologous ∼21-nucleotide (nt) mature miRNA sequences. However, it is unclear whether members of a miRNA gene family, which encode identical or nearly identical mature miRNAs, are functionally interchangeable in vivo. Methods and Findings We show that mir-181a-1, but not mir-181c, can promote CD4 and CD8 double-positive (DP) T cell development when ectopically expressed in thymic progenitor cells. The distinct activities of mir-181a-1 and mir-181c are largely determined by their unique pre-miRNA loop nucleotides—not by the one-nucleotide difference in their mature miRNA sequences. Moreover, the activity of mir-181a-1 on DP cell development can be quantitatively influenced by nucleotide changes in its pre-miRNA loop region. We find that both the strength and the functional specificity of miRNA genes can be controlled by the pre-miRNA loop nucleotides. Intriguingly, we note that mutations in the pre-miRNA loop regions affect pre-miRNA and mature miRNA processing, but find no consistent correlation between the effects of pre-miRNA loop mutations on the levels of mature miRNAs and the activities of the mir-181a-1/c genes. Conclusions These results demonstrate that pre-miRNA loop nucleotides play a critical role in controlling the activity of miRNA genes and that members of the same miRNA gene families could have evolved to achieve different activities via alterations in their pre-miRNA loop sequences, while maintaining identical or nearly identical mature miRNA sequences. PMID:18974849

  11. 46 CFR 45.181 - Load line exemption requirements for the Burns Harbor and Milwaukee routes.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... serviceable condition and comply with the applicable provisions of 46 CFR part 45, subpart E. (d) Expiration... (CONTINUED) LOAD LINES GREAT LAKES LOAD LINES Unmanned River Barges on Lake Michigan Routes § 45.181...

  12. 46 CFR 45.181 - Load line exemption requirements for the Burns Harbor and Milwaukee routes.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... serviceable condition and comply with the applicable provisions of 46 CFR part 45, subpart E. (d) Expiration... (CONTINUED) LOAD LINES GREAT LAKES LOAD LINES Unmanned River Barges on Lake Michigan Routes § 45.181...

  13. 46 CFR 45.181 - Load line exemption requirements for the Burns Harbor and Milwaukee routes.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... serviceable condition and comply with the applicable provisions of 46 CFR part 45, subpart E. (d) Expiration... (CONTINUED) LOAD LINES GREAT LAKES LOAD LINES Unmanned River Barges on Lake Michigan Routes § 45.181...

  14. Regulation of the pT181 encoded tetracycline resistance gene in Straphylococcus aureus

    SciTech Connect

    Mojumdar, M.

    1986-01-01

    pT181 is a naturally-occurring 4437 basepair (bp) plasmid isolated from Staphylococcus aureus which encodes inducible resistance to tetracycline (Tc). The DNA sequence data has identified three open reading frames (ORFs). The largest ORF B, has been found to be responsible for the Tc resistance phenotype of pT181. Since most Tc resistance systems appear to be regulated by an effector protein and a repressor protein, several Bal 31 deletion mutants of pT181 were constructed and analyzed in an effort to identify the elements involved in Tc resistance. Two transcomplementing groups of mutants were identified within the tet gene. The mechanism of Tc resistance was studied by assaying the accumulation of (7-/sup 3/H) Tc by Tc sensitive cells, and uninduced and induced pT181-containing cells. A sharp decrease in accumulation of the drug after an initial increase was observed in Tc induced pT181-containing cells. In vivo labeling of Bacillus subtilis minicells containing pT181 was performed with /sup 35/S-methionine to identify the polypeptide product of the tet gene. A Tc-inducible protein having a molecular weight of approximately 50,000 daltons was detected only in B. subtilis minicells carrying pT181. Cell fractionation studies of S. aureus cells with and without pT181 showed that an approximately 28,000 daltons Tc-inducible protein was present in membranes of pT181 containing cells. The amount of TET protein in Tc induced minicells was about fifteen-fold higher than that in uninduced minicells. RNA prepared from stationary phase cells analyzed by Northern blot hybridization showed that the steady-state level of the tet mRNA in induced pT181-containing cells was bout four-fold higher than that in uninduced pT181-containing cells. When RNA synthesis was blocked with rifampicin, tet mRAN was found to be much more stable in Tc induced cells as compared to that in uninduced cells over a 30 min period.

  15. miR-181a Targets RGS16 to Promote Chondrosarcoma Growth, Angiogenesis, and Metastasis

    PubMed Central

    Sun, Xiaojuan; Charbonneau, Cherie; Wei, Lei; Chen, Qian; Terek, Richard M.

    2015-01-01

    Chondrosarcoma is the most common primary malignant bone tumor in adults, has no effective systemic treatment, and patients with this disease have poor survival. Altered expression of microRNA (miR) is involved in tumorigenesis, however its role in chondrosarcoma is undetermined. MicroRNA-181a is overexpressed in high grade chondrosarcoma, is upregulated by hypoxia, and increases VEGF expression. Here, the purpose was to determine the mechanism of miR-181a regulation of VEGF, determine if miR-181a overexpression promotes tumor progression, and to evaluate an antagomir-based approach for chondrosarcoma treatment. Therapeutic inhibition of miR-181a decreased expression of VEGF and MMP1 in vitro, and angiogenesis, MMP1 activity, tumor growth, and lung metastasis, all by more than 50%, in a xenograft mouse model. A target of miR-181a is regulator of G-protein signaling 16 (RGS16), a negative regulator of CXC chemokine receptor 4 (CXCR4) signaling. CXCR4 signaling is increased in chondrosarcoma, its expression is also increased by hypoxia, and is associated with angiogenesis and metastasis, however, receptor blockade is only partially effective. RGS16 expression is restored after miR-181a inhibition and partially accounts for the anti-angiogenic and anti-metastatic effects of miR-181a inhibition. These data establish miR-181a as an oncomiR that promotes chondrosarcoma progression through a new mechanism involving enhancement of CXCR4 signaling by inhibition of RGS16. PMID:26013170

  16. MiR181c inhibits ovarian cancer metastasis and progression by targeting PRKCD expression

    PubMed Central

    Yao, Lijuan; Wang, Li; Li, Fengxia; Gao, Xihai; Wei, Xuegong; Liu, Zhihui

    2015-01-01

    MicroRNAs (miRNAs) regulate many important cancer related gene expression in the posttranscriptional process. Dysregulated expression of miRNAs has been observed in numerous human cancers including ovarian cancer. In this study, we found that the expression of the miR-181c was significantly decreased in ovarian cancer tissue and in tissues with lymph node metastasis when compared with their control samples, respectively. Moreover, among pathological stages, the expression of miR-181c was significantly decreased in the tissues with IV stage compared with other stages. In vitro, miR-181c significantly inhibited the proliferation, metastasis of A2780 cell line, and induced G1 phase arrest. Through bioinformatics prediction, protein kinase C delta (PRKCD) was identified as a target gene of miR-181c. Western blot results showed that PRKCD was increased in ovarian cancer tissue, in tissues with lymph node metastasis and IV stage of ovarian cancer pathological samples. After knocking down PRKCD, the cell cycle of A2780 cells was also arrested in G1 phase. The proliferation and the metastasis of A2780 cells were reduced. The dual luciferase reporter experiments showed that miR-181c regulated the expression of PRKCD by combining with its 3’UTR. These results indicate that miR-181c inhibits ovarian cancer metastasis and progression by targeting PRKCD expression. PMID:26629004

  17. Noninvasive assessment of tissue distribution and tumor pharmacokinetics of Pc 181, a silicon phthalocyanine analogue, in mice

    NASA Astrophysics Data System (ADS)

    Bai, Lihua; Guo, Jianxia; Clausen, Dana M.; Eiseman, Julie L.

    2010-02-01

    Objective: In in vitro photodynamic therapy, the LD50 of Pc 181 has been reported to be 7 to 8 times less than that of silicon phthalocyanine 4 (Pc 4). The Optical Pharmacokinetic System (OPS) can measure photosensitizer concentrations in accessible tissues non-invasively. We used OPS to evaluate the tumor pharmacokinetics of Pc 181 and Pc 4 and the tissue drug distribution in SCID mice bearing either human breast cancer MDA-MB-231 or human head and neck squamous cell carcinoma SCC-15 xenografts. Methods: Following iv administration of 2.5 mg/kg Pc 181 or 2 mg/kg Pc 4 to SCID mice, OPS measurements were taken on tumor and normal tissues between 5 and 4320 min in vivo or in situ. Results: Large variations in tumor Pc 181 concentrations were observed among mice. In MDA-MB-231 tumors, the Pc 181 concentration peaked at 240 min, and was retained in the tumor. Tumor Pc 181 concentrations were much less than the tumor Pc 4 concentrations at an equimolar dose. Pc 181 concentrations were the highest in liver, followed by spleen, and kidney. In mice bearing SCC-15 xenografts, skin and underlying tissue Pc 181 concentrations were higher than tumor concentrations at all time points examined. Conclusions: This first Pc 181 pharmacokinetics study described a tissue Pc 181 distribution similar to that of Pc 4. However, tumor Pc 181 concentrations were lower than those of Pc 4 at equimolar doses.

  18. Lead Toxicity

    MedlinePlus

    ... homes. • Most people, especially children, who suffer from lead poisoning are exposed through lead-contaminated household dust or ... and six if they are at risk of lead poisoning (see: ). Who can I call to get more ...

  19. Characterization of Three Vasopressin Receptor 2 Variants: An Apparent Polymorphism (V266A) and Two Loss-of-Function Mutations (R181C and M311V)

    PubMed Central

    Armstrong, Stephen P.; Seeber, Ruth M.; Ayoub, Mohammed Akli; Feldman, Brian J.; Pfleger, Kevin D. G.

    2013-01-01

    Arginine vasopressin (AVP) is released from the posterior pituitary and controls water homeostasis. AVP binding to vasopressin V2 receptors (V2Rs) located on kidney collecting duct epithelial cells triggers activation of Gs proteins, leading to increased cAMP levels, trafficking of aquaporin-2 water channels, and consequent increased water permeability and antidiuresis. Typically, loss-of-function V2R mutations cause nephrogenic diabetes insipidus (NDI), whereas gain-of-function mutations cause nephrogenic syndrome of inappropriate antidiuresis (NSIAD). Here we provide further characterization of two mutant V2Rs, R181C and M311V, reported to cause complete and partial NDI respectively, together with a V266A variant, in a patient diagnosed with NSIAD. Our data in HEK293FT cells revealed that for cAMP accumulation, AVP was about 500- or 30-fold less potent at the R181C and M311V mutants than at the wild-type receptor respectively (and about 4000- and 60-fold in COS7 cells respectively). However, in contrast to wild type V2R, the R181C mutant failed to increase inositol phosphate production, while with the M311V mutant, AVP exhibited only partial agonism in addition to a 37-fold potency decrease. Similar responses were detected in a BRET assay for β-arrestin recruitment, with the R181C receptor unresponsive to AVP, and partial agonism with a 23-fold decrease in potency observed with M311V in both HEK293FT and COS7 cells. Notably, the V266A V2R appeared functionally identical to the wild-type receptor in all assays tested, including cAMP and inositol phosphate accumulation, β-arrestin interaction, and in a BRET assay of receptor ubiquitination. Each receptor was expressed at comparable levels. Hence, the M311V V2R retains greater activity than the R181C mutant, consistent with the milder phenotype of NDI associated with this mutant. Notably, the R181C mutant appears to be a Gs protein-biased receptor incapable of signaling to inositol phosphate or recruiting

  20. MicroRNA-181c targets Bcl-2 and regulates mitochondrial morphology in myocardial cells

    PubMed Central

    Wang, Hongjiang; Li, Jing; Chi, Hongjie; Zhang, Fan; Zhu, Xiaoming; Cai, Jun; Yang, Xinchun

    2015-01-01

    Apoptosis is an important mechanism for the development of heart failure. Mitochondria are central to the execution of apoptosis in the intrinsic pathway. The main regulator of mitochondrial pathway of apoptosis is Bcl-2 family which includes pro- and anti-apoptotic proteins. MicroRNAs are small noncoding RNA molecules that regulate gene expression by inhibiting mRNA translation and/or inducing mRNA degradation. It has been proposed that microRNAs play critical roles in the cardiovascular physiology and pathogenesis of cardiovascular diseases. Our previous study has found that microRNA-181c, a miRNA expressed in the myocardial cells, plays an important role in the development of heart failure. With bioinformatics analysis, we predicted that miR-181c could target the 3′ untranslated region of Bcl-2, one of the anti-apoptotic members of the Bcl-2 family. Thus, we have suggested that miR-181c was involved in regulation of Bcl-2. In this study, we investigated this hypothesis using the Dual-Luciferase Reporter Assay System. Cultured myocardial cells were transfected with the mimic or inhibitor of miR-181c. We found that the level of miR-181c was inversely correlated with the Bcl-2 protein level and that transfection of myocardial cells with the mimic or inhibitor of miR-181c resulted in significant changes in the levels of caspases, Bcl-2 and cytochrome C in these cells. The increased level of Bcl-2 caused by the decrease in miR-181c protected mitochondrial morphology from the tumour necrosis factor alpha-induced apoptosis. PMID:25898913

  1. Lead Poisoning

    MedlinePlus

    ... can be found in all parts of our environment. Much of it comes from human activities such as mining and manufacturing. Lead used to be in paint; older houses may still have lead paint. You could be exposed to lead by Eating food or drinking water that contains lead. Water pipes in older homes ...

  2. Lead poisoning

    SciTech Connect

    Rekus, J.F.

    1992-08-01

    Construction workers who weld, cut or blast structural steel coated with lead-based paint are at significant risk of lead poisoning. Although technology to control these exposures may not have existed when the lead standard was promulgated, it is available today. Employers who do not take steps to protect their employees from lead exposure may be cited and fined severely for their failure.

  3. Profiling TRA-1-81 antigen distribution on a human embryonic stem cell

    SciTech Connect

    Qiu Dengli; Xiang Jialing; Li Zhaoxia; Krishnamoorthy, Aparna; Chen Liaohai; Wang Rong

    2008-05-02

    Human embryonic stem (hES) cells hold great promise in regenerative medicine. Although hES cells have unlimited self-renewal potential, they tend to differentiate spontaneously in culture. TRA-1-81 is a biomarker of undifferentiated hES cells. Quantitative characterization of TRA-1-81 expression level in a single cell helps capture the 'turn-on' signal and understand the mechanism of early differentiation. Here, we report on our examination of TRA-1-81 distribution and association on a hES cell membrane using an atomic force microscope (AFM). Our results suggest that aggregated distribution of TRA-1-81 antigen is characteristic for undifferentiated hES cells. We also evaluated the TRA-1-81 expression level at {approx}17,800 epitopes and {approx}700 epitopes per cell on an undifferentiated cell and a spontaneously differentiated cell, respectively. The method in this study can be adapted in examining other surface proteins on various cell types, thus providing a general tool for investigating protein distribution and association at the single cell level.

  4. TGF-β upregulates miR-181a expression to promote breast cancer metastasis

    PubMed Central

    Taylor, Molly A.; Sossey-Alaoui, Khalid; Thompson, Cheryl L.; Danielpour, David; Schiemann, William P.

    2012-01-01

    Late-stage breast cancer metastasis is driven by dysregulated TGF-β signaling, but the underlying molecular mechanisms have not been fully elucidated. We attempted to recapitulate tumor and metastatic microenvironments via the use of biomechanically compliant or rigid 3D organotypic cultures and combined them with global microRNA (miR) profiling analyses to identify miRs that were upregulated in metastatic breast cancer cells by TGF-β. Here we establish miR-181a as a TGF-β–regulated “metastamir” that enhanced the metastatic potential of breast cancers by promoting epithelial-mesenchymal transition, migratory, and invasive phenotypes. Mechanistically, inactivation of miR-181a elevated the expression of the proapoptotic molecule Bim, which sensitized metastatic cells to anoikis. Along these lines, miR-181a expression was essential in driving pulmonary micrometastatic outgrowth and enhancing the lethality of late-stage mammary tumors in mice. Finally, miR-181a expression was dramatically and selectively upregulated in metastatic breast tumors, particularly triple-negative breast cancers, and was highly predictive for decreased overall survival in human breast cancer patients. Collectively, our findings strongly implicate miR-181a as a predictive biomarker for breast cancer metastasis and patient survival, and consequently, as a potential therapeutic target in metastatic breast cancer. PMID:23241956

  5. microRNA-181 promotes prostate cancer cell proliferation by regulating DAX-1 expression.

    PubMed

    Tong, Shi-Jun; Liu, Jun; Wang, Xiang; Qu, Lian-Xi

    2014-10-01

    microRNAs (miRNAs) are a class of short noncoding RNA molecules that have a critical role in the initiation and progression of types of human cancer, including prostate cancer. In the present study, the expression of miR-181 in prostate cancer tissues was evaluated and was demonstrated to be significantly upregulated in prostate cancer tissues compared with that in adjacent normal tissues. The results of in vitro MTT and BrdU incorporation assays, as well as cell-cycle analysis, indicated that miR-181 overexpression markedly promoted the proliferation of LNCaP cells. Furthermore, miR-181 overexpression was found to promote the progression of LNCaP tumor growth in nude mice. Mechanistic studies demonstrated that dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (DAX-1), a negative regulator of androgen receptor in prostate cancer, was inhibited by miR-181 overexpression. Therefore, the results from the present study suggest that miR-181 functions as a growth-suppressive miRNA during prostate cancer development. PMID:25187843

  6. microRNA-181 promotes prostate cancer cell proliferation by regulating DAX-1 expression

    PubMed Central

    TONG, SHI-JUN; LIU, JUN; WANG, XIANG; QU, LIAN-XI

    2014-01-01

    microRNAs (miRNAs) are a class of short noncoding RNA molecules that have a critical role in the initiation and progression of types of human cancer, including prostate cancer. In the present study, the expression of miR-181 in prostate cancer tissues was evaluated and was demonstrated to be significantly upregulated in prostate cancer tissues compared with that in adjacent normal tissues. The results of in vitro MTT and BrdU incorporation assays, as well as cell-cycle analysis, indicated that miR-181 overexpression markedly promoted the proliferation of LNCaP cells. Furthermore, miR-181 overexpression was found to promote the progression of LNCaP tumor growth in nude mice. Mechanistic studies demonstrated that dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1 (DAX-1), a negative regulator of androgen receptor in prostate cancer, was inhibited by miR-181 overexpression. Therefore, the results from the present study suggest that miR-181 functions as a growth-suppressive miRNA during prostate cancer development. PMID:25187843

  7. Lead poisoning.

    PubMed Central

    Landrigan, P J; Todd, A C

    1994-01-01

    Lead poisoning is the most common disease of environmental origin in the United States today. Adult lead poisoning results primarily from exposure by inhalation in the workplace. Pediatric lead poisoning results principally from the ingestion of lead from environmental media, including paint chips, dust, soil, drinking water, ceramics, and medications. Lead is toxic to many organ systems, among them developing erythrocytes, the kidneys, and the nervous system. Lead-induced toxicity to the central nervous system causes delayed development, diminished intelligence, and altered behavior. In young children, this effect has been demonstrated convincingly to occur at blood lead levels between 10 and 20 micrograms per dl. The Centers for Disease Control and Prevention has recommended that a blood lead level of 10 micrograms per dl or higher be considered evidence of increased lead absorption, and the National Academy of Sciences has concurred in that recommendation. Unresolved issues in need of further study include the frequency of screening young children for lead, the question of whether women should be offered screening for lead before conceiving a pregnancy, the role of x-ray fluorescence analysis in assessing lead in bone, and the appropriate legislative response of the United States government to lead-based paint abatement. PMID:7941534

  8. Lead poisoning

    MedlinePlus

    ... lead is still found in some modern faucets. Soil contaminated by decades of car exhaust or years ... house paint scrapings. Lead is more common in soil near highways and houses. Hobbies involving soldering, stained ...

  9. Temperature dependence of the electric field gradient at 181Ta in nanostructured HfO2 film

    NASA Astrophysics Data System (ADS)

    Cavalcante, F. H. C.; Carbonari, A. W.; Soares, J. C.

    2010-11-01

    The hyperfine field at metal lattice sites in a nanostructured HfO2 thin film was studied by the Perturbed Angular Correlation (PAC) technique using 181Ta as probe nuclei. The thin oxide film was deposited by pulsed laser ablation on a silicon substrate kept at 673 K. The resulting film thickness was about 25 nm. The 181Ta isotopes are formed in Hf sites of the sample as disintegration product of radioactive 181Hf, which were produced by neutron activation of the very thin film by the reaction 180Hf(n,γ)181Hf.. PAC measurements of the electric field gradient at the 181Ta probe sites .were performed with the thin film in the temperature range 295 - 1273 K in vacuum. Results showed that the film has a single crystalline structure and two different quadrupole frequencies could be observed corresponding to 181Ta at monoclinic structure and with oxygen vacancy in its neighborhood.

  10. Interaction of 11B with 209Bi and 181Ta targets at intermediate energies

    NASA Astrophysics Data System (ADS)

    Karapetyan, G. S.; Deppman, A.; Guimarães, V.; Balabekyan, A.; Demekhina, N. A.

    2016-08-01

    Production cross sections for nuclei fragments from the interaction of 11B ions with 209Bi and 181Ta targets, in the intermediate energy regime, have been obtained with the induced-activity method and offline γ spectroscopy. The mass-yield distributions were obtained and the fission process was investigated by using the multimodal fission approach in the case of the 209Bi target and a pure symmetric distribution for the 181Ta target. The fissilities were deduced for both targets and further compared to the data for proton-induced reactions. The relative values of the linear momentum imparted to the targets were derived from the cross section data.

  11. 26 CFR 1.501(c)(18)-1 - Certain funded pension trusts.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 26 Internal Revenue 7 2011-04-01 2009-04-01 true Certain funded pension trusts. 1.501(c)(18)-1... pension trusts. (a) In general. Organizations described in section 501(c)(18) are trusts created before June 25, 1959, forming part of a plan for the payment of benefits under a pension plan funded only...

  12. 19 CFR 181.100 - Effect of NAFTA advance ruling letters; modification and revocation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Effect of NAFTA advance ruling letters... Advance Ruling Procedures § 181.100 Effect of NAFTA advance ruling letters; modification and revocation. (a) Effect of NAFTA advance ruling letters—(1) General. An advance ruling letter issued by...

  13. 26 CFR 1.501(c)(18)-1 - Certain funded pension trusts.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 7 2010-04-01 2010-04-01 true Certain funded pension trusts. 1.501(c)(18)-1... pension trusts. (a) In general. Organizations described in section 501(c)(18) are trusts created before... contributions of employees. In order to be exempt, such trusts must also meet the requirements set forth...

  14. 19 CFR 181.23 - Effect of noncompliance; failure to provide documentation regarding transshipment.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Effect of noncompliance; failure to provide... Import Requirements § 181.23 Effect of noncompliance; failure to provide documentation regarding transshipment. (a) Effect of noncompliance. If the importer fails to comply with any requirement under this...

  15. 26 CFR 1.181-2 - Election to deduct production costs.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... applicable, if a significant portion of the total number of days of first-unit principal photography will... principal photography engaged in) for the applicable activities described in § 1.181-1(b)(2)(ii), (b)(2)(iii... number of days of first-unit principal photography engaged in) for such activities (whether or not...

  16. 26 CFR 1.181-2 - Election to deduct production costs.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... applicable, if a significant portion of the total number of days of first-unit principal photography will... principal photography engaged in) for the applicable activities described in § 1.181-1(b)(2)(ii), (b)(2)(iii... number of days of first-unit principal photography engaged in) for such activities (whether or not...

  17. 26 CFR 1.181-2 - Election to deduct production costs.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... applicable, if a significant portion of the total number of days of first-unit principal photography will... principal photography engaged in) for the applicable activities described in § 1.181-1(b)(2)(ii), (b)(2)(iii... number of days of first-unit principal photography engaged in) for such activities (whether or not...

  18. 19 CFR 181.21 - Filing of claim for preferential tariff treatment upon importation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) NORTH AMERICAN FREE TRADE AGREEMENT..., or equivalent documentation, including electronic submissions, the symbol “CA” for a good of Canada... qualifying good is classified. Except as otherwise provided in 19 CFR 181.22 and except in the case of a...

  19. 29 CFR 4.181 - Overtime pay provisions of other Acts.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Standards Overtime Pay of Covered Employees § 4.181 Overtime pay provisions of other Acts. (a) Fair Labor... requiring compliance with the overtime provisions of the Fair Labor Standards Act, contractors and... overtime pay standards of the Fair Labor Standards Act. This is true with respect to......

  20. 29 CFR 4.181 - Overtime pay provisions of other Acts.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Standards Overtime Pay of Covered Employees § 4.181 Overtime pay provisions of other Acts. (a) Fair Labor... requiring compliance with the overtime provisions of the Fair Labor Standards Act, contractors and... overtime pay standards of the Fair Labor Standards Act. This is true with respect to......

  1. 29 CFR 4.181 - Overtime pay provisions of other Acts.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Standards Overtime Pay of Covered Employees § 4.181 Overtime pay provisions of other Acts. (a) Fair Labor... requiring compliance with the overtime provisions of the Fair Labor Standards Act, contractors and... overtime pay standards of the Fair Labor Standards Act. This is true with respect to......

  2. 29 CFR 4.181 - Overtime pay provisions of other Acts.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Standards Overtime Pay of Covered Employees § 4.181 Overtime pay provisions of other Acts. (a) Fair Labor... requiring compliance with the overtime provisions of the Fair Labor Standards Act, contractors and... overtime pay standards of the Fair Labor Standards Act. This is true with respect to......

  3. 29 CFR 4.181 - Overtime pay provisions of other Acts.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Standards Overtime Pay of Covered Employees § 4.181 Overtime pay provisions of other Acts. (a) Fair Labor... requiring compliance with the overtime provisions of the Fair Labor Standards Act, contractors and... overtime pay standards of the Fair Labor Standards Act. This is true with respect to......

  4. 36 CFR 18.1 - What is the authority and purpose for this part?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., DEPARTMENT OF THE INTERIOR LEASING OF PROPERTIES IN PARK AREAS § 18.1 What is the authority and purpose for this part? 16 U.S.C. 1 et seq., particularly 16 U.S.C. 1a-2(k), and, 16 U.S.C. 470h-3 are...

  5. Munc18-1-regulated stage-wise SNARE assembly underlying synaptic exocytosis

    PubMed Central

    Ma, Lu; Rebane, Aleksander A; Yang, Guangcan; Xi, Zhiqun; Kang, Yuhao; Gao, Ying; Zhang, Yongli

    2015-01-01

    Synaptic-soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins couple their stage-wise folding/assembly to rapid exocytosis of neurotransmitters in a Munc18-1-dependent manner. The functions of the different assembly stages in exocytosis and the role of Munc18-1 in SNARE assembly are not well understood. Using optical tweezers, we observed four distinct stages of assembly in SNARE N-terminal, middle, C-terminal, and linker domains (or NTD, MD, CTD, and LD, respectively). We found that SNARE layer mutations differentially affect SNARE assembly. Comparison of their effects on SNARE assembly and on exocytosis reveals that NTD and CTD are responsible for vesicle docking and fusion, respectively, whereas MD regulates SNARE assembly and fusion. Munc18-1 initiates SNARE assembly and structures t-SNARE C-terminus independent of syntaxin N-terminal regulatory domain (NRD) and stabilizes the half-zippered SNARE complex dependent upon the NRD. Our observations demonstrate distinct functions of SNARE domains whose assembly is intimately chaperoned by Munc18-1. DOI: http://dx.doi.org/10.7554/eLife.09580.001 PMID:26701912

  6. 21 CFR 181.26 - Drying oils as components of finished resins.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.26 Drying oils as components of finished resins. Substances... 21 Food and Drugs 3 2013-04-01 2013-04-01 false Drying oils as components of finished resins....

  7. 21 CFR 181.26 - Drying oils as components of finished resins.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.26 Drying oils as components of finished resins. Substances... 21 Food and Drugs 3 2012-04-01 2012-04-01 false Drying oils as components of finished resins....

  8. 21 CFR 181.22 - Certain substances employed in the manufacture of food-packaging materials.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... OF HEALTH AND HUMAN SERVICES (CONTINUED) PRIOR-SANCTIONED FOOD INGREDIENTS Specific Prior-Sanctioned Food Ingredients § 181.22 Certain substances employed in the manufacture of food-packaging materials... 21 Food and Drugs 3 2014-04-01 2014-04-01 false Certain substances employed in the manufacture...

  9. 26 CFR 1.181-1T - Deduction for qualified film and television production costs (temporary).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... of the cost of acquiring or developing screenplays, scripts, story outlines, motion picture... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Deduction for qualified film and television... and Corporations (continued) § 1.181-1T Deduction for qualified film and television production...

  10. 26 CFR 1.181-3T - Qualified film or television production (temporary).

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 26 Internal Revenue 3 2010-04-01 2010-04-01 false Qualified film or television production... (continued) § 1.181-3T Qualified film or television production (temporary). (a) In general. The term qualified film or television production means any production (as defined in paragraph (b) of this...

  11. 14 CFR 121.181 - Airplanes: Reciprocating engine-powered: En route limitations: One engine inoperative.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 14 Aeronautics and Space 3 2012-01-01 2012-01-01 false Airplanes: Reciprocating engine-powered: En... OPERATIONS Airplane Performance Operating Limitations § 121.181 Airplanes: Reciprocating engine-powered: En... person operating a reciprocating engine powered airplane may take off that airplane at a weight,...

  12. 14 CFR 121.181 - Airplanes: Reciprocating engine-powered: En route limitations: One engine inoperative.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false Airplanes: Reciprocating engine-powered: En... OPERATIONS Airplane Performance Operating Limitations § 121.181 Airplanes: Reciprocating engine-powered: En... person operating a reciprocating engine powered airplane may take off that airplane at a weight,...

  13. 14 CFR 121.181 - Airplanes: Reciprocating engine-powered: En route limitations: One engine inoperative.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 14 Aeronautics and Space 3 2013-01-01 2013-01-01 false Airplanes: Reciprocating engine-powered: En... OPERATIONS Airplane Performance Operating Limitations § 121.181 Airplanes: Reciprocating engine-powered: En... person operating a reciprocating engine powered airplane may take off that airplane at a weight,...

  14. Brodie's abscess. A study of 181 cases, with special reference to radiographic diagnostic criteria.

    PubMed

    Boriani, S

    1980-12-01

    A study of 181 cases of Brodie's abscess, in which the diagnosis was histologically confirmed, was made in order to demonstrate radiographic factors common to other conditions, and therefore likely to pose questions of differential diagnosis. Some of the pathological characteristics of Brodie's abscess are described and correlated with the differing radiographic appearances of this inflammatory bone lesion.

  15. Leading Democratically

    ERIC Educational Resources Information Center

    Brookfield, Stephen

    2010-01-01

    Democracy is the most venerated of American ideas, the one for which wars are fought and people die. So most people would probably agree that leaders should be able to lead well in a democratic society. Yet, genuinely democratic leadership is a relative rarity. Leading democratically means viewing leadership as a function or process, rather than…

  16. Cerebrospinal Fluid P-Tau181P: Biomarker for Improved Differential Dementia Diagnosis.

    PubMed

    Struyfs, Hanne; Niemantsverdriet, Ellis; Goossens, Joery; Fransen, Erik; Martin, Jean-Jacques; De Deyn, Peter P; Engelborghs, Sebastiaan

    2015-01-01

    The goal of this study is to investigate the value of tau phosphorylated at threonine 181 (P-tau181P) in the Alzheimer's disease (AD) cerebrospinal fluid (CSF) biomarker panel for differential dementia diagnosis in autopsy confirmed AD and non-AD patients. The study population consisted of 140 autopsy confirmed AD and 77 autopsy confirmed non-AD dementia patients. CSF concentrations of amyloid-β peptide of 42 amino acids (Aβ1-42), total tau protein (T-tau), and P-tau181P were determined with single analyte ELISA-kits (INNOTEST(®), Fujirebio, Ghent, Belgium). Diagnostic accuracy was assessed through receiver operating characteristic (ROC) curve analyses to obtain area under the curve (AUC) values and to define optimal cutoff values to discriminate AD from pooled and individual non-AD groups. ROC curve analyses were only performed on biomarkers and ratios that differed significantly between the groups. Pairwise comparison of AUC values was performed by means of DeLong tests. The Aβ1-42/P-tau181P ratio (AUC = 0.770) performed significantly better than Aβ1-42 (AUC = 0.677, P = 0.004), T-tau (AUC = 0.592, P < 0.001), and Aβ1-42/T-tau (AUC = 0.678, P = 0.001), while P-tau181P (AUC = 0.720) performed significantly better than T-tau (AUC = 0.592, P < 0.001) to discriminate between AD and the pooled non-AD group. When comparing AD and the individual non-AD diagnoses, Aβ1-42/P-tau181P (AUC = 0.894) discriminated AD from frontotemporal dementia significantly better than Aβ1-42 (AUC = 0.776, P = 0.020) and T-tau (AUC = 0.746, P = 0.004), while P-tau181P/T-tau (AUC = 0.958) significantly improved the differentiation between AD and Creutzfeldt-Jakob disease as compared to Aβ1-42 (AUC = 0.688, P = 0.004), T-tau (AUC = 0.874, P = 0.040), and Aβ1-42/P-tau181P (AUC = 0.760, P = 0.003). In conclusion, this study demonstrates P-tau181P is an essential component of the AD CSF biomarker

  17. SY 18-1 TRANSLATIONAL RESEARCH IN PAH.

    PubMed

    Chung, Wook-Jin

    2016-09-01

    Pulmonary arterial hypertension (PAH) is a life-threatening disorder with a poor prognosis and causes pulmonary vascular remodeling accompanied with increased pulmonary arterial medial wall thickness and fibrosis, which leads to vascular and right ventricular (RV) dysfunction. Despite treatment with prostacyclin, endothelin antagonist, and phosphodiesterase-5 inhibitors the 1-year mortality rate of PAH still remains high. Recent registries, clinical trials, and basic researches have been increasing the knowledge of PAH and it would contribute to potential therapeutic strategies and better clinical outcome.Korean Registry of Pulmonary Arterial Hypertension (KORPAH) is the first modern PAH registries in Asian ethnicity. Total 39 centers participated and 625 patients were enrolled. This study evaluated the incidence, prevalence, epidemiology, therapeutic modalities and survival data of Korean patients with PAH."Gachon experiences" was to characterize the clinical outcomes and evaluate the factors influencing survival time of the PAH patients in Korean. This study compared the cumulative survival of total 43 PAH patients who received targeted or conventional therapy.PAH Ilopost BMPR-2 gene in Korea IIT Multi-institutional (PILGRIM) is a prospective, investigator-initiative, and multi-institutional clinical trials. This study was recently completed in March by 7 institutes, and aimed to investigate (1) the prevalence of BMPR-2 gene mutations in the Korean PAH patients and (2) the effect of iloprost inhalation solution on hemodynamic response, and exercise echocardiography.PAH basic research focuses on two major themes: (1) Systematic comparison of the effects of adipose tissue, bone marrow and umbilical cord blood-derived mesenchymal stem cell transplantation on MCT-induced PAH in rats and (2) investigation of the effect of human UCB-derived MSC (hUCB-MSC) transplantation combined with apelin-13 administration on MCT-induced PAH in rats. Data suggests that, although the

  18. 19 CFR 181.64 - Goods re-entered after repair or alteration in Canada or Mexico.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Canada or Mexico. 181.64 Section 181.64 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF... in Canada or Mexico. (a) General. This section sets forth the rules which apply for purposes of... Mexico as provided for in subheadings 9802.00.40 and 9802.00.50, HTSUS. Goods returned after having...

  19. 9 CFR 381.181 - Reports by consignees of allegedly adulterated or misbranded products; sale or transportation as...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 2 2011-01-01 2011-01-01 false Reports by consignees of allegedly adulterated or misbranded products; sale or transportation as violations. 381.181 Section 381.181 Animals and Animal Products FOOD SAFETY AND INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE AGENCY ORGANIZATION...

  20. Iodine-125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA-181c expression

    PubMed Central

    Yang, Yong; Ma, Zhen-Huan; Li, Xiao-Gang; Zhang, Wan-Fu; Wan, Jia; Du, Ling-Juan; Li, Guo-Jian; Yang, Guo-Kai; Lu, Ping

    2016-01-01

    Iodine-125 (125I) seed implantation has been widely used for the treatment of unresectable advanced tumors. However, the molecular mechanisms underlying the tumor-suppressive effects of 125I irradiation have not been fully elucidated. The present study demonstrated that 125I irradiation suppresses cell viability and inhibits cell invasiveness of gastric cancer KATO-III and MKN45 cells. Further mechanistic analysis suggested the involvement of microRNA (miR)-181c in the inhibitory effects induced by 125I irradiation. Methylated DNA immunoprecipitation coupled with quantitative-polymerase chain reaction demonstrated that treatment with 125I irradiation, at the dose of 4 Gy, induced promoter demethylation of the miR-181c gene in KATO-III and MKN45 cells. Following irradiation, the expression of miR-181c was significantly increased, which may be attributed to the demethylation caused by 125I irradiation. In addition, upregulation of miR-181c by administration of miR-181c mimics decreased cell invasion, suggesting the role of miR-181c as a tumor suppressor. More importantly, the tumor-suppressive effects of 125I irradiation were significantly compromised by the introduction of miR-181c inhibitors. Overall, these results reveal that 125I irradiation inhibits invasiveness of gastric cancer cells by reactivating miR-181c at the epigenetic level, thereby providing important molecular evidence for the anticancer effects of 125I irradiation. PMID:27698859

  1. Iodine-125 irradiation inhibits invasion of gastric cancer cells by reactivating microRNA-181c expression

    PubMed Central

    Yang, Yong; Ma, Zhen-Huan; Li, Xiao-Gang; Zhang, Wan-Fu; Wan, Jia; Du, Ling-Juan; Li, Guo-Jian; Yang, Guo-Kai; Lu, Ping

    2016-01-01

    Iodine-125 (125I) seed implantation has been widely used for the treatment of unresectable advanced tumors. However, the molecular mechanisms underlying the tumor-suppressive effects of 125I irradiation have not been fully elucidated. The present study demonstrated that 125I irradiation suppresses cell viability and inhibits cell invasiveness of gastric cancer KATO-III and MKN45 cells. Further mechanistic analysis suggested the involvement of microRNA (miR)-181c in the inhibitory effects induced by 125I irradiation. Methylated DNA immunoprecipitation coupled with quantitative-polymerase chain reaction demonstrated that treatment with 125I irradiation, at the dose of 4 Gy, induced promoter demethylation of the miR-181c gene in KATO-III and MKN45 cells. Following irradiation, the expression of miR-181c was significantly increased, which may be attributed to the demethylation caused by 125I irradiation. In addition, upregulation of miR-181c by administration of miR-181c mimics decreased cell invasion, suggesting the role of miR-181c as a tumor suppressor. More importantly, the tumor-suppressive effects of 125I irradiation were significantly compromised by the introduction of miR-181c inhibitors. Overall, these results reveal that 125I irradiation inhibits invasiveness of gastric cancer cells by reactivating miR-181c at the epigenetic level, thereby providing important molecular evidence for the anticancer effects of 125I irradiation.

  2. LEAD STUDIES

    PubMed Central

    Aub, Joseph C.; Reznikoff, Paul; Smith, Dorothea E.

    1924-01-01

    It appears, from the investigations in other laboratories, that the anemia observed in cases of lead poisoning is due to destruction of blood rather than to diminished production of blood. The method of poisoning cells in vitro with lead was adopted in order to study this phenomenon, and distinct effects were thereby obtained, even when only 0.001 mg. of lead is added to approximately 5 billion washed red corpuscles. In order to obtain optimum results the usual dosage employed was ten times this or 0.01 mg. per 5 billion cells. The following changes were observed in cells so treated. 1. Such a marked increase in the resistance to hypotonic salt solution develops that complete hemolysis does not occur until the cells are exposed to a saline solution of 0.05 per cent. Untreated cells are completely hemolyzed in 0.25 or 0.225 per cent saline. 2. This reaction is quantitative and varies with the concentration of lead used. Under the conditions of our experiments this phenomenon seems to be unique. The effects of arsenic are very slight in comparison. 3. While from this reaction it may be concluded that lead increases cellular resistance, it also appears that it shortens the life of blood cells. This may be demonstrated by the much more rapid appearance of hemolysis than normal when the cells are merely allowed to stand in Ringer solution of any dilution. 4. In rabbits with acute lead poisoning these same phenomena may be noted in vivo. 5. Both phenomena may be changed in vitro by varying the time and temperature of the reaction and the concentration of lead, as Fici has already pointed out. 6. If normal cells stand in Ringer solution for 6 hours something diffuses into the solution which largely reduces the action of lead. After repeated washing these cells react with lead in the usual manner. 7. Small amounts of serum react with lead and eliminate its effects. Red blood cells, treated with a mixture of lead and blood serum, show normal hemolysis in hypotonic salt

  3. The replication initiator protein of plasmid pT181 has sequence-specific endonuclease and topoisomerase-like activities.

    PubMed Central

    Koepsel, R R; Murray, R W; Rosenblum, W D; Khan, S A

    1985-01-01

    Initiation of pT181 DNA replication specifically requires the plasmid-encoded RepC protein. Here we demonstrate that highly purified RepC protein has sequence-specific endonuclease and topoisomerase-like activities. A maximum sequence of 127 base pairs containing the pT181 origin of replication is required for nicking-closing by RepC protein. RepC introduces a single strand break within the pT181 origin. The nick site has been shown by DNA sequencing to lie between nucleotides 70 and 71 in the bottom strand of the DNA within the origin sequence. This nick site probably corresponds to the start site of pT181 replication. The results presented here suggest that, unlike most other plasmids, pT181 replicates by a rolling circle mechanism. Images PMID:2995991

  4. Functional analysis of miR-181a and Fas involved in hepatitis B virus-related hepatocellular carcinoma pathogenesis

    SciTech Connect

    Zou, Chengcheng; Chen, Juan; Chen, Ke; Wang, Sen; Cao, Yiyi; Zhang, Jinnan; Sheng, Yanrui; Huang, Ailong; Tang, Hua

    2015-02-15

    The hepatitis B virus (HBV) is responsible for most of hepatocellular carcinoma (HCC). However, whether HBV plays an important role during hepatocarcinogenesis through effecting miRNAs remains unknown. Here, we reported that HBV up-regulated microRNA-181a (miR-181a) by enhancing its promoter activity. Simultaneously, we found that miR-181a inhibited apoptosis in vitro and promoted tumor cell growth in vivo. TNF receptor superfamily member 6 (Fas) was further identified as a target of miR-181a. We also found that Fas could reverse the apoptosis-inhibition effect induced by miR-181a. Moreover, HBV could inhibit cell apoptosis by down-regulating Fas expression, which could be reversed by miR-181a inhibitor. Our data demonstrated that HBV suppressed apoptosis of hepatoma cells by up-regulating miR-181a expression and down-regulating Fas expression, which may provide a new understanding of the mechanism in HBV-related HCC pathogenesis. - Highlights: • HBV could up-regulate miR-181a expression by interacting with nt−800 to +240 in its promoter region in HCC cell lines. • HBV could down-regulate Fas expression and suppress apoptosis of hepatoma cells, which could be reversed by miR-181a inhibitor. • Up-regulation of miR-181a promoted proliferation of hepatoma cells and repressed apoptosis, which could be reversed by Fas. • Our study provides a new understanding of the mechanism in HBV-related HCC pathogenesis.

  5. MicroRNA Bta-miR-181a regulates the biosynthesis of bovine milk fat by targeting ACSL1.

    PubMed

    Lian, S; Guo, J R; Nan, X M; Ma, L; Loor, J J; Bu, D P

    2016-05-01

    MicroRNA (miRNA) are a class of small noncoding RNA that function as important posttranscriptional regulators of gene expression. The acyl-CoA synthetase long-chain family member 1 (ACSL1) is an important enzyme in the process of milk lipid synthesis. In a previous study dealing with incubations of stearic acid in bovine mammary epithelial cells, an opposite expression pattern was observed between ACSL1 and miR-181a. Bioinformatics analysis with TargetScan and PicTar revealed ACSL1 as a potential target gene of miR-181a. The objective of this work was to determine the potential function of miR-181a on milk fat synthesis by defining the regulatory relationship between miR-181a and ACSL1. Primary bovine mammary epithelial cells were harvested from mid-lactation cows and cultured in Dulbecco's modified Eagle's medium/F-12 medium with 10% fetal bovine serum, 0.5μg/mL of insulin, 10 ng/mL of epidermal growth factor, 5μg/mL of transferrin, 1μg/mL of hydrocortisone, 1μg/mL of progesterone, 5μg/mL of estradiol, and 5μg/mL of prolactin. Cells were transfected with an miR-181a mimic to increase its expression and an miR-181a inhibitor to decrease its expression before culturing for 48 h. The results revealed that the overexpression of miR-181a inhibited the expression of ACSL1, whereas the downregulation of miR-181a increased ACSL1 expression. Western blot analysis of ACSL1 revealed similar effects. Oil-red-O staining indicated that cellular lipid droplet synthesis was decreased with the overexpression of bta-miR-181a, and treatment with the bta-miR-181a inhibitor increased concentration of lipid droplets. Furthermore, overexpression of bta-miR-181a resulted in a decrease in concentration of triacylglycerol in the cells, whereas inhibition of bta-miR-181a increased concentration of triacylglycerol. Therefore, the results indicated that bta-miR-181a may contribute to negative regulation of lipid synthesis in mammary cells via targeting ACSL1. PMID:26971144

  6. MicroRNA Bta-miR-181a regulates the biosynthesis of bovine milk fat by targeting ACSL1.

    PubMed

    Lian, S; Guo, J R; Nan, X M; Ma, L; Loor, J J; Bu, D P

    2016-05-01

    MicroRNA (miRNA) are a class of small noncoding RNA that function as important posttranscriptional regulators of gene expression. The acyl-CoA synthetase long-chain family member 1 (ACSL1) is an important enzyme in the process of milk lipid synthesis. In a previous study dealing with incubations of stearic acid in bovine mammary epithelial cells, an opposite expression pattern was observed between ACSL1 and miR-181a. Bioinformatics analysis with TargetScan and PicTar revealed ACSL1 as a potential target gene of miR-181a. The objective of this work was to determine the potential function of miR-181a on milk fat synthesis by defining the regulatory relationship between miR-181a and ACSL1. Primary bovine mammary epithelial cells were harvested from mid-lactation cows and cultured in Dulbecco's modified Eagle's medium/F-12 medium with 10% fetal bovine serum, 0.5μg/mL of insulin, 10 ng/mL of epidermal growth factor, 5μg/mL of transferrin, 1μg/mL of hydrocortisone, 1μg/mL of progesterone, 5μg/mL of estradiol, and 5μg/mL of prolactin. Cells were transfected with an miR-181a mimic to increase its expression and an miR-181a inhibitor to decrease its expression before culturing for 48 h. The results revealed that the overexpression of miR-181a inhibited the expression of ACSL1, whereas the downregulation of miR-181a increased ACSL1 expression. Western blot analysis of ACSL1 revealed similar effects. Oil-red-O staining indicated that cellular lipid droplet synthesis was decreased with the overexpression of bta-miR-181a, and treatment with the bta-miR-181a inhibitor increased concentration of lipid droplets. Furthermore, overexpression of bta-miR-181a resulted in a decrease in concentration of triacylglycerol in the cells, whereas inhibition of bta-miR-181a increased concentration of triacylglycerol. Therefore, the results indicated that bta-miR-181a may contribute to negative regulation of lipid synthesis in mammary cells via targeting ACSL1.

  7. Ecotoxicology: Lead

    USGS Publications Warehouse

    Scheuhammer, A.M.; Beyer, W.N.; Schmitt, C.J.; Jorgensen, Sven Erik; Fath, Brian D.

    2008-01-01

    Lead (Pb) is a naturally occurring metallic element; trace concentrations are found in all environmental media and in all living things. However, certain human activities, especially base metal mining and smelting; combustion of leaded gasoline; the use of Pb in hunting, target shooting, and recreational angling; the use of Pb-based paints; and the uncontrolled disposal of Pb-containing products such as old vehicle batteries and electronic devices have resulted in increased environmental levels of Pb, and have created risks for Pb exposure and toxicity in invertebrates, fish, and wildlife in some ecosystems.

  8. Tetraethyl lead

    Integrated Risk Information System (IRIS)

    Tetraethyl lead ; CASRN 78 - 00 - 2 Human health assessment information on a chemical substance is included in the IRIS database only after a comprehensive review of toxicity data , as outlined in the IRIS assessment development process . Sections I ( Health Hazard Assessments for Noncarcinogenic Ef

  9. Selective CDK7 inhibition with BS-181 suppresses cell proliferation and induces cell cycle arrest and apoptosis in gastric cancer

    PubMed Central

    Wang, Bo-Yong; Liu, Quan-Yan; Cao, Jun; Chen, Ji-Wei; Liu, Zhi-Su

    2016-01-01

    Cyclin-dependent kinase (CDK) family members have been considered as attractive therapeutic targets for cancer. In this study, we aim to investigate the anticancer effects of a selective CDK7 inhibitor, BS-181, in gastric cancer (GC) cell line. Human GC cells (BGC823) were cultured with or without BS-181 at different concentrations for 24–72 hours. BS-181 significantly reduced the activity of CDK7 with downregulation of cyclin D1 and XIAP in GC cells. Treatment with BS-181 induced cell cycle arrest and apoptosis. The expression of Bax and caspase-3 was significantly increased, while Bcl-2 expression was decreased in cells treated with BS-181. In addition, the inhibition of CDK7 with BS-181 resulted in reduced rates of proliferation, migration, and invasion of gastric cells. Those results demonstrated the anticancer activities of selective CDK7 inhibitor BS-181 in BGC823 cells, suggesting that CDK7 may serve as a novel therapeutic target or the treatment of GC. PMID:27042010

  10. miR-181a is a negative regulator of GRIA2 in methamphetamine-use disorder

    PubMed Central

    Zhang, Kai; Wang, Qingzhong; Jing, Xuxiu; Zhao, Yan; Jiang, Haifeng; Du, Jiang; Yu, Shunying; Zhao, Min

    2016-01-01

    A previous study reported that the miR-181a level in serum was significantly different between patients with methamphetamine-use disorder and healthy controls and that chronic methamphetamine use down-regulates the expression of miR-181a. Bioinformatic analysis predicted that miR-181a might bind the 3′-UTRs of the mRNA transcripts of the human glutamate receptor genes GRIA2 and GABRA1. In this study, we measured the expression of GRIA2 and GABRA1 in patients with methamphetamine-use disorder. In addition, we examined whether miR-181a down-regulates GRIA2 and GABRA1 in a cell-based assay. We further examined the effects of chronic methamphetamine exposure on the expression of miR-181a, GRIA2 and GABRA1. The results demonstrated that serum GRIA2 is higher in patients with methamphetamine-use disorder than in healthy controls. Dual luciferase reporter assays and a cell-based model of methamphetamine exposure also showed that miR-181a directly regulates expression of GRIA2. This study supports the evidence that miR-181a and the glutamate AMPA receptor gene GRIA2 play a critical role in methamphetamine-use disorder. PMID:27767084

  11. miR-181a induces sorafenib resistance of hepatocellular carinoma cells through downregulation of RASSF1 expression.

    PubMed

    Azumi, Junya; Tsubota, Toshiaki; Sakabe, Tomohiko; Shiota, Goshi

    2016-09-01

    Sorafenib, a multi-kinase inhibitor, is the only standard clinical drug for patients with advanced hepatocellular carcinoma (HCC); however, development of sorafenib resistance in HCC often prevents its long-term efficacy. Therefore, novel targets and strategies are urgently needed to improve the antitumor effect of sorafenib. In the present study, we examined the novel mechanisms of sorafenib resistance of HCC cells by investigating the difference in sorafenib sensitivity between two HCC cell lines. Sorafenib induced more apoptosis of HepG2 cells compared to Hep3B cells. Sorafenib exposure to HepG2 cells but not Hep3B cells increased the expression of proapoptotic factor PUMA, and activated PARP and caspase-3. Notably, microRNA-181a (miR-181a) expression levels were lower in HepG2 cells than in Hep3B cells. Exogenous miR-181a expression in HepG2 cells reduced apoptosis, whereas inhibition of miR-181a in Hpe3B cells increased apoptosis. In addition, we demonstrated that miR-181a directly targets RASSF1, a MAPK signaling factor, and knockdown of RASSF1 increased sorafenib resistance. Taken together, these results suggest that miR-181a provokes sorafenib resistance through suppression of RASSF1. Our data provide important insight into the novel therapeutic strategy against sorafenib resistance of HCC cells by targeting of miR-181a pathway. PMID:27384977

  12. Lysophosphatidic acid (LPA) 18:1 transcriptional regulation of primary human gingival fibroblasts.

    PubMed

    Cerutis, D Roselyn; Weston, Michael D; Ogunleye, Afolabi O; McVaney, Timothy P; Miyamoto, Takanari

    2014-12-01

    The pleiotropic, bioactive lipid lysophosphatidic acid [(LPA), 1-acyl-sn-glycerol-3-phosphate] exerts critical regulatory actions in physiology and pathophysiology in many systems. It is present in normal bodily fluids, and is elevated in pathology (1). In vivo, "LPA" exists as distinct molecular species, each having a single fatty acid of varying chain length and degree of unsaturation covalently attached to the glycerol backbone via an acyl, alkyl, or alkenyl link. These species differ in affinities for the individual LPA receptors [(LPARs), LPA1-6] and coupling to G proteins (2). However, LPA 18:1 has been and continues to be the most commonly utilized species in reported studies. The actions of "LPA" remain poorly defined in oral biology and pathophysiology. Our laboratory has addressed this knowledge gap by studying in vitro the actions of the major human salivary LPA species [18:1, 18:0, and 16:0 (3)] in human oral cells (4-7). This includes gingival fibroblasts (GF), which our flow cytometry data from multiple donors found that they express LPA1-5 (6). We have also reported that these species are ten-fold elevated to pharmacologic levels in the saliva and gingival crevicular fluid obtained from patients with moderate-severe periodontitis (8). As the potential of LPA to regulate transcriptional activity had not been examined in the oral system, this study used whole human genome microarray analysis to test the hypothesis that LPA 18:1-treated human GF would show significant changes in gene transcripts relevant to their biology, wound-healing, and inflammatory responses. LPA 18:1 was found to significantly regulate a large, complex set of genes critical to GF biology in these categories and to periodontal disease. The raw data has been deposited at NCBI's GEO database as record GSE57496. PMID:26484133

  13. 19 CFR 181.21 - Filing of claim for preferential tariff treatment upon importation.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., or the symbol “MX” for a good of Mexico, as a prefix to the subheading of the HTSUS under which each qualifying good is classified. Except as otherwise provided in 19 CFR 181.22 and except in the case of a good to which Appendix 6.B to Annex 300-B of the NAFTA applies (see also 19 CFR 102.25), the...

  14. 19 CFR 181.21 - Filing of claim for preferential tariff treatment upon importation.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., or the symbol “MX” for a good of Mexico, as a prefix to the subheading of the HTSUS under which each qualifying good is classified. Except as otherwise provided in 19 CFR 181.22 and except in the case of a good to which Appendix 6.B to Annex 300-B of the NAFTA applies (see also 19 CFR 102.25), the...

  15. 19 CFR 181.21 - Filing of claim for preferential tariff treatment upon importation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., or the symbol “MX” for a good of Mexico, as a prefix to the subheading of the HTSUS under which each qualifying good is classified. Except as otherwise provided in 19 CFR 181.22 and except in the case of a good to which Appendix 6.B to Annex 300-B of the NAFTA applies (see also 19 CFR 102.25), the...

  16. 19 CFR 181.21 - Filing of claim for preferential tariff treatment upon importation.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., or the symbol “MX” for a good of Mexico, as a prefix to the subheading of the HTSUS under which each qualifying good is classified. Except as otherwise provided in 19 CFR 181.22 and except in the case of a good to which Appendix 6.B to Annex 300-B of the NAFTA applies (see also 19 CFR 102.25), the...

  17. Reduced CSF p-Tau181 to Tau ratio is a biomarker for FTLD-TDP

    PubMed Central

    Watts, Kelly; Grossman, Murray; Glass, Jonathan; Lah, James J.; Hales, Chadwick; Shelnutt, Matthew; Van Deerlin, Vivianna; Trojanowski, John Q.; Levey, Allan I.

    2013-01-01

    Objectives: To validate the ability of candidate CSF biomarkers to distinguish between the 2 main forms of frontotemporal lobar degeneration (FTLD), FTLD with TAR DNA-binding protein 43 (TDP-43) inclusions (FTLD-TDP) and FTLD with Tau inclusions (FTLD-Tau). Methods: Antemortem CSF samples were collected from 30 patients with FTLD in a single-center validation cohort, and CSF levels of 5 putative FTLD-TDP biomarkers as well as levels of total Tau (t-Tau) and Tau phosphorylated at threonine 181 (p-Tau181) were measured using independent assays. Biomarkers most associated with FTLD-TDP were then tested in a separate 2-center validation cohort composed of subjects with FTLD-TDP, FTLD-Tau, Alzheimer disease (AD), and cognitively normal subjects. The sensitivity and specificity of FTLD-TDP biomarkers were determined. Results: In the first validation cohort, FTLD-TDP cases had decreased levels of p-Tau181 and interleukin-23, and increased Fas. Reduced ratio of p-Tau181 to t-Tau (p/t-Tau) was the strongest predictor of FTLD-TDP pathology. Analysis in the second validation cohort showed CSF p/t-Tau ratio <0.37 to distinguish FTLD-TDP from FTLD-Tau, AD, and healthy seniors with 82% sensitivity and 82% specificity. Conclusion: A reduced CSF p/t-Tau ratio represents a reproducible, validated biomarker for FTLD-TDP with performance approaching well-established CSF AD biomarkers. Introducing this biomarker into research and the clinical arena can significantly increase the power of clinical trials targeting abnormal accumulations of TDP-43 or Tau, and select the appropriate patients for target-specific therapies. Classification of evidence: This study provides Class II evidence that the CSF p/t-Tau ratio distinguishes FTLD-TDP from FTLD-Tau. PMID:24174584

  18. Magnetic properties of Cu70.9Al18.1Mn11 alloy

    NASA Astrophysics Data System (ADS)

    Chatterjee, S.; Majumdar, S.

    2013-02-01

    The ferromagnetic shape memory alloy of nominal composition Cu70.9Al18.1Mn11 has been investigated through dc and ac magnetization measurements. The studied alloy undergoes ferromagnetic to glassy transition below martensitic transition. Clear frequency shift in ac susceptibility measurement is observed, which actually indicates the spin glass freezing in the sample. The studied alloy also shows constricted hysteresis loop at 5 K.

  19. Estimation of Tau and Phosphorylated Tau181 in Serum of Alzheimer’s Disease and Mild Cognitive Impairment Patients

    PubMed Central

    Shekhar, Shashank; Kumar, Rahul; Rai, Nitish; Kumar, Vijay; Singh, Kusum; Upadhyay, Ashish Datt; Tripathi, Manjari; Dwivedi, Sadanand; Dey, Aparajit B.; Dey, Sharmistha

    2016-01-01

    The elevated level of cerebrospinal fluid (CSF) Tau and phosphorylated Tau181 (p-Tau181) proteins are well established hallmarks of Alzheimer’s disease (AD). Elevated level of p-Tau181 can differentiate AD from other neurodegenerative disease. However, the expression level of these proteins in serum of AD patient is not well set up. This study sought to evaluate the level of Tau and p-Tau181 in serum of AD, and mild cognitive impairment (MCI) patients for an alternative approach to establish protein-based markers by convenient way. Blood samples were collected from 39 AD patients, 37 MCI patients and 37 elderly individuals as controls. The levels of Tau and p-Tau181 in the serum of the different groups were measured by label free real time Surface Plasmon Resonance technology by using specific antibodies, and were further confirmed by the conventional western blot method. An appropriate statistical analysis, including Receiver Operating Characteristic (ROC), was performed. The concentrations of serum Tau and p-Tau181 were significantly higher (p<0.00001) in AD (Tau; 47.49±9.00ng/μL, p-Tau181; 0.161±0.04 ng/μL) compared to MCI (Tau; 39.26±7.78 ng/μL, p-Tau181; 0.135±0.02 ng/μL) and were further higher compared to elderly controls (Tau; 34.92±6.58 ng/μL, p-Tau181; 0.122±0.01 ng/ μL). A significant (p<0.0001) downhill correlation was found between Tau as well as p-Tau181 levels with HMSE and MoCA score. This study for the first time reports the concentration of Tau and p-Tau181 in serum of AD and MCI patients. The cutoff values of Tau and p-Tau181 of AD and MCI patients with sensitivity and specificity reveal that serum level of these proteins can be used as a predictive marker for AD and MCI. PMID:27459603

  20. 49 CFR 40.181 - What does the second laboratory do with the split specimen when it is tested to reconfirm a...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 1 2014-10-01 2014-10-01 false What does the second laboratory do with the split specimen when it is tested to reconfirm a substituted test result? 40.181 Section 40.181 Transportation... TESTING PROGRAMS Split Specimen Tests § 40.181 What does the second laboratory do with the split...