Sample records for lead 194

  1. Lead Poisoning Prevention Tips

    MedlinePlus

    ... be done to prevent exposure to lead? Lead-Safe Work Coloring book Reduce a child’s exposure from non- ... here to print a coloring storybook on lead-safe work [PDF - 1.94 MB] . Top of Page To ...

  2. The possibility of establishing causes of death on the basis of the exhumed remains of prisoners executed during the communist regime in Poland: the exhumations at Powązki Military Cemetery in Warsaw.

    PubMed

    Szleszkowski, Łukasz; Thannhäuser, Agata; Szwagrzyk, Krzysztof; Jurek, Tomasz

    2015-07-01

    This study presents the results of the analysis of forensic examinations of the remains of 194 prisoners exhumed at Powązki Military Cemetery in Warsaw. In all probability, most of those buried there were judicially sentenced to death by firing squad or hanging in connection with activities of the Polish independence underground in its struggles with the postwar communist regime. Forensic medical research focussed on determining causes of death and reconstructing the mechanisms of injury leading to death. Most probable causes of death were found in 108 of 194 cases; of these, 76 were isolated gunshot wounds to the head, mostly directed to the occipital region. In 29 of 194 cases, only extensive skull fractures were observed, making it impossible to determine the mechanism of injury. The condition of these skulls do not permit the exclusion of injuries due to gunshots, which were very likely given the historical context of the studied location. In one case, it is assumed that the cause of death could be blunt force trauma to the head. In 86 of 194 cases, it was not possible to determine the cause of death. Of these cases, 20 skeletons were in such poor condition that erosive changes could have completely obliterated even very extensive head injuries leading to death. No injuries were observed that could be associated with execution by hanging.

  3. Rational Design of Cyclic Antimicrobial Peptides Based on BPC194 and BPC198.

    PubMed

    Cirac, Anna D; Torné, Maria; Badosa, Esther; Montesinos, Emilio; Salvador, Pedro; Feliu, Lidia; Planas, Marta

    2017-06-24

    A strategy for the design of antimicrobial cyclic peptides derived from the lead compounds c(KKLKKFKKLQ) ( BPC194 ) and c(KLKKKFKKLQ) ( BPC198 ) is reported. First, the secondary β-structure of BPC194 and BPC198 was analyzed by carrying out molecular dynamics (MD) simulations. Then, based on the sequence pattern and the β-structure of BPC194 or BPC198 , fifteen analogues were designed and synthesized on solid-phase. The best peptides ( BPC490 , BPC918, and BPC924 ) showed minimum inhibitory concentration (MIC) values <6.2 μM against Pseudomonas syringae pv. syringae and Xanthomonas axonopodis pv. vesicatoria , and an MIC value of 12.5 to 25 μM against Erwinia amylovora , being as active as BPC194 and BPC198 . Interestingly, these three analogues followed the structural pattern defined from the MD simulations of the parent peptides. Thus, BPC490 maintained the parallel alignment of the hydrophilic pairs K¹-K⁸, K²-K⁷, and K⁴-K⁵, whereas BPC918 and BPC924 included the two hydrophilic interactions K³-Q 10 and K⁵-K⁸. In short, MD simulations have proved to be very useful for ascertaining the structural features of cyclic peptides that are crucial for their biological activity. Such approaches could be further employed for the development of new antibacterial cyclic peptides.

  4. A prospective study of XRCC1 haplotypes and their interaction with plasma carotenoids on breast cancer risk

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mohrenweiser, H W; Han, J; Hankinson, S E

    2004-01-15

    The XRCC1 protein is involved in the base excision repair pathway through interactions with other proteins. Polymorphisms in the XRCC1 gene may lead to variation in repair proficiency and confer inherited predisposition to cancer. We prospectively assessed the associations between polymorphisms and haplotypes in XRCC1 and breast cancer risk in a nested case-control study within the Nurses' Health Study (incident cases, n 1004; controls, n 1385). We further investigated gene-environment interactions between the XRCC1 variations and plasma carotenoids on breast cancer risk. We genotyped four haplotype-tagging single nucleotide polymorphisms Arg {sup 194}Trp, C26602T, Arg{sup 399}Gln, and Gln{sup 632}Gln in themore » XRCC1 gene. Five common haplotypes accounted for 99% of the chromosomes in the present study population of mostly Caucasian women. We observed a marginally significant reduction in the risk of breast cancer among {sup 194}Trp carriers. As compared with no-carriers, women with at least one {sup 194}Trp allele had a multivariate odds ratio of 0.79 (95% of the confidence interval, 0.60 -1.04). The inferred haplotype harboring the {sup 194}Trp allele was more common in controls than in cases (6.6 versus 5.3%, P 0.07). We observed that the Arg {sup 194}Trp modified the inverse associations of plasma -carotene level (P, ordinal test for interaction 0.02) and plasma -carotene level (P, ordinal test for interaction 0.003) with breast cancer risk. No suggestion of an interaction was observed between the Arg {sup 194}Trp and cigarette smoking. Our results suggest an inverse association between XRCC1 {sup 194}Trp allele and breast cancer risk. The findings of the effect modification of the Arg {sup 194}Trp on the relations of plasma -and -carotene levels with breast cancer risk suggest a potential protective effect of carotenoids in breast carcinogenesis by preventing oxidative DNA damage.« less

  5. Structure-function relationships for the selective inhibition of human 3β-hydroxysteroid dehydrogenase type 1 by a novel androgen analog.

    PubMed

    Pham, Jenny H; Will, Catherine M; Mack, Vance L; Halbert, Matthew; Conner, Edward Alexander; Bucholtz, Kevin M; Thomas, James L

    2017-11-01

    3β-Hydroxysteroid dehydrogenase type 1 (3β-HSD1) is selectively expressed in human placenta, mammary glands and breast tumors in women. Human 3β-HSD2 is selectively expressed in adrenal glands and ovaries. Based on AutoDock 3 and 4 results, we have exploited key differences in the amino acid sequences of 3β-HSD1 (Ser194, Arg195) and 3β-HSD2 (Gly194, Pro195) by designing a selective inhibitor of 3β-HSD1. 2,16-Dicyano-4,5-epoxy-androstane-3,17-dione (16-cyano-17-keto-trilostane or DiCN-AND) was synthesized in a 4-step procedure from androstenedione. In purified 3β-HSD inhibition studies, DiCN-AND competitively inhibited 3β- HSD1 with K i =4.7μM and noncompetitively inhibited 3β-HSD2 with a 6.5-fold higher K i =30.7μM. We previously reported similar isoenzyme-specific inhibition profiles for trilostane. Based on our docking results, we created, expressed and purified the chimeric S194G-1 mutant of 3β-HSD1. Trilostane inhibited S194G-1 (K i =0.67μM) with a noncompetitive mode compared to its 6.7-fold higher affinity, competitive inhibition of 3β-HSD1 (K i =0.10μM). DiCN-AND inhibited S194G-1 with a 6.3-fold higher K i (29.5μM) than measured for 3β-HSD1 (K i =4.7μM) but with the same competitive mode for both enzyme species. Since DiCN-AND noncompetitively inhibits 3β-HSD2, which has the Gly194 and Pro195 of 3β-HSD2 in place of the Ser194 and Arg195 in 3β-HSD1, this suggests that Arg195 alone in 3β-HSD1 or S194G-1 is required to bind DiCN-AND in the substrate binding site (competitive inhibition). However, both Ser194 and Arg195 are required to bind trilostane in the 3β-HSD1 substrate site based on its noncompetitive inhibition of S194G-1 and 3β-HSD2. In support of this hypothesis, DiCN-AND inhibited our chimeric R195P-1 mutant noncompetitively with a K i =41.3μM (similar to the 3β-HSD2 inhibition profile). Since DiCN-AND competitively inhibited S194G-1 that still contains R195 but noncompetitively inhibited R195P-1 that still contains S194, our data provides strong evidence that the Arg195 being mutated to Pro195 (as present in 3β-HSD2) shifts the inhibition mode from competitive to noncompetitive in 3β-HSD1. This supports the key role of Arg195 in 3β-HSD1 for the high affinity, competitive binding of the trilostane analogs. Our new structure/function information for the design of targeted 3β-HSD1 inhibitors may lead to important new treatments for the prevention of spontaneous premature birth. Copyright © 2017 Elsevier Ltd. All rights reserved.

  6. Students' Value Orientations, Intuitive Decision Making, and Motivational Interference, and Their Relations to Regret

    ERIC Educational Resources Information Center

    Kuhnle, Claudia; Sinclair, Marta; Hofer, Manfred; Kilian, Britta

    2014-01-01

    Students' learning activities frequently compete with their leisure options, leading to regret after decisions to study. Using a sample of 233 German and 194 Australian undergraduate students, the authors explored possible determinants of the personality construct regret. They investigated whether the level to which students rely on intuition in…

  7. Heavy metal contaminants in yerberia shop products.

    PubMed

    Levine, Michael; Mihalic, Jason; Ruha, Anne-Michelle; French, Robert N E; Brooks, Daniel E

    2013-03-01

    Complementary and alternative medications, including the use of herbal medications, have become quite popular in the USA. Yerberias are found throughout the southwest and specialize in selling Hispanic herbal products. The products sold in these stores are not regulated by any governmental agency. Previous reports have found Ayurvedic medications contain high levels of lead, mercury, and arsenic. The primary purpose of this study is to examine the prevalence of heavy metal contaminants sold at Yerberia stores in the southwest. Yerberias in the Phoenix, Arizona area were identified via search of an on-line search engine using the words "Yerberia Phoenix." Every second store was selected, and products were purchased using a standard script. The products were subsequently analyzed for mercury, lead, and arsenic. The main outcome is the prevalence of heavy metal content in over-the-counter "cold" medications purchased at a Yerberia. Twenty-two samples were purchased. One product contained pure camphor (2-camphone) and was subsequently not further analyzed. Of the 21 samples analyzed, lead was found in 4/21 (19.4 %). Arsenic and mercury were in 1/21 (4.8 %) each. Because two samples contained two heavy metals, the total prevalence of heavy metals was 4/21 (19.4). Heavy metal contaminants are commonly encountered in over-the-counter herbal "cold" medications purchased at Yerberias in the southwest.

  8. Structural signatures of DRD4 mutants revealed using molecular dynamics simulations: Implications for drug targeting.

    PubMed

    Jatana, Nidhi; Thukral, Lipi; Latha, N

    2016-01-01

    Human Dopamine Receptor D4 (DRD4) orchestrates several neurological functions and represents a target for many psychological disorders. Here, we examined two rare variants in DRD4; V194G and R237L, which elicit functional alterations leading to disruption of ligand binding and G protein coupling, respectively. Using atomistic molecular dynamics (MD) simulations, we provide in-depth analysis to reveal structural signatures of wild and mutant complexes with their bound agonist and antagonist ligands. We constructed intra-protein network graphs to discriminate the global conformational changes induced by mutations. The simulations also allowed us to elucidate the local side-chain dynamical variations in ligand-bound mutant receptors. The data suggest that the mutation in transmembrane V (V194G) drastically disrupts the organization of ligand binding site and causes disorder in the native helical arrangement. Interestingly, the R237L mutation leads to significant rewiring of side-chain contacts in the intracellular loop 3 (site of mutation) and also affects the distant transmembrane topology. Additionally, these mutations lead to compact ICL3 region compared to the wild type, indicating that the receptor would be inaccessible for G protein coupling. Our findings thus reveal unreported structural determinants of the mutated DRD4 receptor and provide a robust framework for design of effective novel drugs.

  9. MicroRNA-194 promotes osteoblast differentiation via downregulating STAT1

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Li, Jun; He, Xijing; Wei, Wenzhi

    Osteoblast differentiation is a vital process in maintaining bone homeostasis in which various transcriptional factors, signaling molecules, and microRNAs (miRNAs) are involved. Recently, signal transducer and activator of transcription 1 (STAT1) has been found to play an important role in regulating osteoblast differentiation. Here, we identified that STAT1 expression was regulated by miR-194. Using mouse bone mesenchymal stem cells (BMSCs), we found that miR-194 expression was significantly increased following osteoblast differentiation induction. Overexpression of miR-194 by lentivirus-mediated gene transfer markedly increased osteoblast differentiation, whereas inhibition of miR-194 significantly suppressed osteoblast differentiation of BMSCs. Using a dual-luciferase reporter assay, a directmore » interaction between miR-194 and the 3′-untranslated region (UTR) of STAT1 was confirmed. Additionally, miR-194 regulated mRNA and protein expression of STAT1 in BMSCs. Further analysis showed that miR-194 overexpression promoted the nuclear translocation of runt-related transcription factor 2 (Runx2), which is critical for osteoblast differentiation. In contrast, inhibition of miR-194 blocked the nuclear translocation of Runx2. Moreover, overexpression of STAT1 significantly blocked Runx2 nuclear translocation and osteoblast differentiation mediated by miR-194 overexpression. Taken together, our data suggest that miR-194 regulates osteoblast differentiation through modulating STAT1-mediated Runx2 nuclear translocation. - Highlights: • Overexpression of miR-194 significantly increased osteoblast differentiation. • miR-194 directly targeted the 3′- UTR of STAT1. • miR-194 regulated the expression of STAT1. • Overexpression of miR-194 promoted the nuclear translocation of Runx2.« less

  10. 49 CFR 194.113 - Information summary.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false Information summary. 194.113 Section 194.113... Response Plans § 194.113 Information summary. (a) The information summary for the core plan, required by... state(s). (b) The information summary for the response zone appendix, required in § 194.107, must...

  11. 40 CFR 194.27 - Peer review.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Peer review. 194.27 Section 194.27... § 194.27 Peer review. (a) Any compliance application shall include documentation of peer review that has... barrier evaluation as required in § 194.44. (b) Peer review processes required in paragraph (a) of this...

  12. 41 CFR 102-194.5 - What is the Standard and Optional Forms Management Program?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What is the Standard and Optional Forms Management Program? 102-194.5 Section 102-194.5 Public Contracts and Property Management... PROGRAMS 194-STANDARD AND OPTIONAL FORMS MANAGEMENT PROGRAM § 102-194.5 What is the Standard and Optional...

  13. 40 CFR 264.194 - General operating requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ....194 Section 264.194 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) SOLID WASTES (CONTINUED) STANDARDS FOR OWNERS AND OPERATORS OF HAZARDOUS WASTE TREATMENT, STORAGE, AND DISPOSAL FACILITIES Tank Systems § 264.194 General operating requirements. (a) Hazardous wastes or treatment reagents must...

  14. 40 CFR 194.15 - certification application(s).

    Code of Federal Regulations, 2010 CFR

    1996-07-01

    ... 40 PROTECTION OF ENVIRONMENT 12 1996-07-01 1996-07-01 false certification application(s). 194.15 Sec. 194.15 Content of compliance re PROTECTION OF ENVIRONMENT ENVIRONMENTAL PROTECTION AGENCY... and Re-certification Applications Sec. 194.15 Content of compliance re-certification application(s...

  15. 40 CFR 194.55 - Results of compliance assessments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Results of compliance assessments. 194.55 Section 194.55 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Individual and Ground-Water Protection Requirements § 194.55 Results of compliance assessments. (a...

  16. 40 CFR 194.55 - Results of compliance assessments.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Results of compliance assessments. 194.55 Section 194.55 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Individual and Ground-Water Protection Requirements § 194.55 Results of compliance assessments. (a...

  17. 40 CFR 194.34 - Results of performance assessments.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Results of performance assessments. 194.34 Section 194.34 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Containment Requirements § 194.34 Results of performance assessments. (a) The results of performance...

  18. 40 CFR 194.34 - Results of performance assessments.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Results of performance assessments. 194.34 Section 194.34 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Containment Requirements § 194.34 Results of performance assessments. (a) The results of performance...

  19. 40 CFR 194.55 - Results of compliance assessments.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Results of compliance assessments. 194.55 Section 194.55 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Individual and Ground-Water Protection Requirements § 194.55 Results of compliance assessments. (a...

  20. 40 CFR 194.34 - Results of performance assessments.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Results of performance assessments. 194.34 Section 194.34 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Containment Requirements § 194.34 Results of performance assessments. (a) The results of performance...

  1. 40 CFR 194.34 - Results of performance assessments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Results of performance assessments. 194.34 Section 194.34 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Containment Requirements § 194.34 Results of performance assessments. (a) The results of performance...

  2. 40 CFR 194.55 - Results of compliance assessments.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Results of compliance assessments. 194.55 Section 194.55 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Individual and Ground-Water Protection Requirements § 194.55 Results of compliance assessments. (a...

  3. 40 CFR 194.55 - Results of compliance assessments.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Results of compliance assessments. 194.55 Section 194.55 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Individual and Ground-Water Protection Requirements § 194.55 Results of compliance assessments. (a...

  4. 40 CFR 194.34 - Results of performance assessments.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Results of performance assessments. 194.34 Section 194.34 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Containment Requirements § 194.34 Results of performance assessments. (a) The results of performance...

  5. 40 CFR 194.32 - Scope of performance assessments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Scope of performance assessments. 194.32 Section 194.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Containment Requirements § 194.32 Scope of performance assessments. (a) Performance assessments shall consider...

  6. 40 CFR 194.51 - Consideration of protected individual.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Consideration of protected individual. 194.51 Section 194.51 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Individual and Ground-Water Protection Requirements § 194.51 Consideration of protected individual...

  7. 40 CFR 194.51 - Consideration of protected individual.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Consideration of protected individual. 194.51 Section 194.51 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Individual and Ground-Water Protection Requirements § 194.51 Consideration of protected individual...

  8. 40 CFR 194.51 - Consideration of protected individual.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Consideration of protected individual. 194.51 Section 194.51 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Individual and Ground-Water Protection Requirements § 194.51 Consideration of protected individual...

  9. 40 CFR 194.51 - Consideration of protected individual.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Consideration of protected individual. 194.51 Section 194.51 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION... Individual and Ground-Water Protection Requirements § 194.51 Consideration of protected individual...

  10. Original Research: miR-194 inhibits proliferation and invasion and promotes apoptosis by targeting KDM5B in esophageal squamous cell carcinoma cells.

    PubMed

    Cui, Guanghui; Liu, Donglei; Li, Weihao; Li, Yuhang; Liang, Youguang; Shi, Wensong; Zhao, Song

    2017-01-01

    Increasing evidence suggests that miR-194 is down-regulated in esophageal squamous cell carcinoma tumor tissue. However, the role and underlying mechanism of miR-194 in esophageal squamous cell carcinoma have not been well defined. We used DIANA, TargetScan and miRanda to perform target prediction analysis and found KDM5B is a potential target of miR-194. Based on these findings, we speculated that miR-194 might play a role in esophageal squamous cell carcinoma development and progression by regulation the expression of KDM5B. We detected the expression of miR-194 and KDM5B by quantitative real-time reverse transcription PCR (qRT-PCR) and Western blot assays, respectively, and found down-regulation of miR-194 and up-regulation of KDM5B existed in esophageal squamous cell carcinoma cell lines. By detecting proliferation, invasion and apoptosis of TE6 and TE14 cells transfected with miR-194 mimics or mimic control, miR-194 was found to inhibit proliferation and invasion and promote apoptosis of esophageal squamous cell carcinoma cells. miR-194 was further verified to regulate proliferation, apoptosis and invasion of esophageal squamous cell carcinoma cells by directly targeting KDM5B. Furthermore, animal studies were performed and showed that overexpression of miR-194 inhibited the growth of esophageal squamous cell carcinoma tumors in vivo. These results confirmed our speculation that miR-194 targets KDM5B to inhibit esophageal squamous cell carcinoma development and progression. These findings offer new clues for esophageal squamous cell carcinoma development and progression and novel potential therapeutic targets for esophageal squamous cell carcinoma. © 2016 by the Society for Experimental Biology and Medicine.

  11. 40 CFR 194.3 - Communications.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Communications. 194.3 Section 194.3... FOR THE CERTIFICATION AND RE-CERTIFICATION OF THE WASTE ISOLATION PILOT PLANT'S COMPLIANCE WITH THE 40 CFR PART 191 DISPOSAL REGULATIONS General Provisions § 194.3 Communications. (a) Compliance...

  12. 40 CFR 194.3 - Communications.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Communications. 194.3 Section 194.3... CFR PART 191 DISPOSAL REGULATIONS General Provisions § 194.3 Communications. (a) Compliance...) Communications and reports concerning the criteria in this part shall be: (1) Addressed to the Administrator or...

  13. 30 CFR 19.4 - Conditions governing investigations.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Conditions governing investigations. 19.4 Section 19.4 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR TESTING, EVALUATION, AND APPROVAL OF MINING PRODUCTS ELECTRIC CAP LAMPS § 19.4 Conditions governing investigations. (a...

  14. 46 CFR 194.15-5 - Ventilation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Ventilation. 194.15-5 Section 194.15-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194...

  15. 40 CFR 194.3 - Communications.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Communications. 194.3 Section 194.3... FOR THE CERTIFICATION AND RE-CERTIFICATION OF THE WASTE ISOLATION PILOT PLANT'S COMPLIANCE WITH THE 40 CFR PART 191 DISPOSAL REGULATIONS General Provisions § 194.3 Communications. (a) Compliance...

  16. 40 CFR 194.3 - Communications.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Communications. 194.3 Section 194.3... CFR PART 191 DISPOSAL REGULATIONS General Provisions § 194.3 Communications. (a) Compliance...) Communications and reports concerning the criteria in this part shall be: (1) Addressed to the Administrator or...

  17. 10 CFR 72.194 - Physical requirements.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 2 2011-01-01 2011-01-01 false Physical requirements. 72.194 Section 72.194 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT... Certification of Personnel § 72.194 Physical requirements. The physical condition and the general health of...

  18. 10 CFR 72.194 - Physical requirements.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 2 2012-01-01 2012-01-01 false Physical requirements. 72.194 Section 72.194 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT... Certification of Personnel § 72.194 Physical requirements. The physical condition and the general health of...

  19. 10 CFR 72.194 - Physical requirements.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 2 2013-01-01 2013-01-01 false Physical requirements. 72.194 Section 72.194 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT... Certification of Personnel § 72.194 Physical requirements. The physical condition and the general health of...

  20. 10 CFR 72.194 - Physical requirements.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 2 2014-01-01 2014-01-01 false Physical requirements. 72.194 Section 72.194 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT... Certification of Personnel § 72.194 Physical requirements. The physical condition and the general health of...

  1. 46 CFR 194.15-5 - Ventilation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Ventilation. 194.15-5 Section 194.15-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194...

  2. 46 CFR 194.15-3 - Responsibility.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Responsibility. 194.15-3 Section 194.15-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194...

  3. [Characteristics and identification of bacteriocins produced by Lactococcus lactis subsp. lactis 194-K].

    PubMed

    Ustiugova, E A; Timofeeva, A V; Stoianova, L G; Netrusov, A I; Katrukha, G S

    2012-01-01

    The Lactococcus lactis subsp. lactis 194-K strain has been established to be able to produce two bacteriocins, one of which was identified as the known lantibiotic nisin A, and the other 194-D bacteriocin represents a polypeptide with a 2589-Da molecular mass and comprises 20 amino acid residues. Both bacteriocins were produced in varying proportions in all of the studied nutrient media, which support the growth of the producer. Depending on the cultivation medium, the nisin A content was 380- to 1123-fold lower in the 194-K stain culture fluid than that of the 194-D peptide. In comparision to to nisin A Bacteriocin 194-D possessed a wide range of antibacterial activity and suppressed the growth of both Gram-positive and Gram-negative bacteria. An optimal medium for 194-D bacteriocin synthesis was shown to be a fermentation medium which contained yeast extract, casein hydrolysate, and potassium phosphate. The biosynthesis ofbacteriocin 194-D by the 194-K strain in these media occurred parallel to producer growth, and its maximal accumulation in the culture fluid was observed at 14-20 h of the strain's growth.

  4. 21 CFR 211.194 - Laboratory records.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 4 2014-04-01 2014-04-01 false Laboratory records. 211.194 Section 211.194 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS: GENERAL CURRENT GOOD MANUFACTURING PRACTICE FOR FINISHED PHARMACEUTICALS Records and Reports § 211.194...

  5. 21 CFR 211.194 - Laboratory records.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 4 2012-04-01 2012-04-01 false Laboratory records. 211.194 Section 211.194 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS: GENERAL CURRENT GOOD MANUFACTURING PRACTICE FOR FINISHED PHARMACEUTICALS Records and Reports § 211.194...

  6. 21 CFR 211.194 - Laboratory records.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 4 2011-04-01 2011-04-01 false Laboratory records. 211.194 Section 211.194 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS: GENERAL CURRENT GOOD MANUFACTURING PRACTICE FOR FINISHED PHARMACEUTICALS Records and Reports § 211.194...

  7. 21 CFR 211.194 - Laboratory records.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 4 2013-04-01 2013-04-01 false Laboratory records. 211.194 Section 211.194 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS: GENERAL CURRENT GOOD MANUFACTURING PRACTICE FOR FINISHED PHARMACEUTICALS Records and Reports § 211.194...

  8. 49 CFR 194.117 - Training.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Training. 194.117 Section 194.117 Transportation... Response Plans § 194.117 Training. (a) Each operator shall conduct training to ensure that: (1) All... of equipment, fire suits, and breathing apparatus. (b) Each operator shall maintain a training record...

  9. 49 CFR 194.117 - Training.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 3 2011-10-01 2011-10-01 false Training. 194.117 Section 194.117 Transportation... Response Plans § 194.117 Training. (a) Each operator shall conduct training to ensure that: (1) All... of equipment, fire suits, and breathing apparatus. (b) Each operator shall maintain a training record...

  10. 46 CFR 194.15-1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false General. 194.15-1 Section 194.15-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-1 General...

  11. 46 CFR 194.15-9 - Storage.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Storage. 194.15-9 Section 194.15-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-9 Storage...

  12. 46 CFR 194.15-9 - Storage.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Storage. 194.15-9 Section 194.15-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-9 Storage...

  13. 46 CFR 194.15-1 - General.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false General. 194.15-1 Section 194.15-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-1 General...

  14. 46 CFR 194.15-9 - Storage.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Storage. 194.15-9 Section 194.15-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-9 Storage...

  15. 46 CFR 194.15-9 - Storage.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Storage. 194.15-9 Section 194.15-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-9 Storage...

  16. 46 CFR 194.15-9 - Storage.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Storage. 194.15-9 Section 194.15-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-9 Storage...

  17. Overexpression of OsCYP19-4 increases tolerance to cold stress and enhances grain yield in rice (Oryza sativa).

    PubMed

    Yoon, Dae Hwa; Lee, Sang Sook; Park, Hyun Ji; Lyu, Jae Il; Chong, Won Seog; Liu, Jang Ryol; Kim, Beom-Gi; Ahn, Jun Cheul; Cho, Hye Sun

    2016-01-01

    AtCYP19-4 (also known as CYP5) was previously identified as interacting in vitro with GNOM, a member of a large family of ARF guanine nucleotide exchange factors that is required for proper polar localization of the auxin efflux carrier PIN1. The present study demonstrated that OsCYP19-4, a gene encoding a putative homologue of AtCYP19-4, was up-regulated by several stresses and showed over 10-fold up-regulation in response to cold. The study further demonstrated that the promoter of OsCYP19-4 was activated in response to cold stress. An OsCYP19-4-GFP fusion protein was targeted to the outside of the plasma membrane via the endoplasmic reticulum as determined using brefeldin A, a vesicle trafficking inhibitor. An in vitro assay with a synthetic substrate oligomer confirmed that OsCYP19-4 had peptidyl-prolyl cis-trans isomerase activity, as was previously reported for AtCYP19-4. Rice plants overexpressing OsCYP19-4 showed cold-resistance phenotypes with significantly increased tiller and spike numbers, and consequently enhanced grain weight, compared with wild-type plants. Based on these results, the authors suggest that OsCYP19-4 is required for developmental acclimation to environmental stresses, especially cold. Furthermore, the results point to the potential of manipulating OsCYP19-4 expression to enhance cold tolerance or to increase biomass. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

  18. 10 CFR 72.194 - Physical requirements.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Physical requirements. 72.194 Section 72.194 Energy... Certification of Personnel § 72.194 Physical requirements. The physical condition and the general health of... considered in the selection of personnel for activities that are important to safety. These conditions need...

  19. 19 CFR 10.194 - Evidence of direct shipment.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 1 2010-04-01 2010-04-01 false Evidence of direct shipment. 10.194 Section 10.194 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY ARTICLES CONDITIONALLY FREE, SUBJECT TO A REDUCED RATE, ETC. Caribbean Basin Initiative § 10.194...

  20. 40 CFR 194.22 - Quality assurance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Quality assurance. 194.22 Section 194... General Requirements § 194.22 Quality assurance. (a)(1) As soon as practicable after April 9, 1996, the Department shall adhere to a quality assurance program that implements the requirements of ASME NQA-1-1989...

  1. 49 CFR 572.194 - Shoulder.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 7 2013-10-01 2013-10-01 false Shoulder. 572.194 Section 572.194 Transportation..., Small Adult Female § 572.194 Shoulder. (a) The shoulder structure is part of the upper torso assembly shown in drawing 180-3000. For the shoulder impact test, the dummy is tested as a complete assembly...

  2. 49 CFR 572.194 - Shoulder.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 7 2012-10-01 2012-10-01 false Shoulder. 572.194 Section 572.194 Transportation..., Small Adult Female § 572.194 Shoulder. (a) The shoulder structure is part of the upper torso assembly shown in drawing 180-3000. For the shoulder impact test, the dummy is tested as a complete assembly...

  3. 49 CFR 572.194 - Shoulder.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 7 2014-10-01 2014-10-01 false Shoulder. 572.194 Section 572.194 Transportation..., Small Adult Female § 572.194 Shoulder. (a) The shoulder structure is part of the upper torso assembly shown in drawing 180-3000. For the shoulder impact test, the dummy is tested as a complete assembly...

  4. 49 CFR 572.194 - Shoulder.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 7 2011-10-01 2011-10-01 false Shoulder. 572.194 Section 572.194 Transportation..., Small Adult Female § 572.194 Shoulder. (a) The shoulder structure is part of the upper torso assembly shown in drawing 180-3000. For the shoulder impact test, the dummy is tested as a complete assembly...

  5. 46 CFR 194.20-9 - Storage.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Storage. 194.20-9 Section 194.20-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemical Stores and/or Storerooms § 194.20-9 Storage. (a) Chemical...

  6. 46 CFR 194.20-9 - Storage.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Storage. 194.20-9 Section 194.20-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemical Stores and/or Storerooms § 194.20-9 Storage. (a) Chemical...

  7. 46 CFR 194.20-9 - Storage.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Storage. 194.20-9 Section 194.20-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemical Stores and/or Storerooms § 194.20-9 Storage. (a) Chemical...

  8. 46 CFR 194.20-9 - Storage.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Storage. 194.20-9 Section 194.20-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemical Stores and/or Storerooms § 194.20-9 Storage. (a) Chemical...

  9. 46 CFR 194.20-9 - Storage.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Storage. 194.20-9 Section 194.20-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemical Stores and/or Storerooms § 194.20-9 Storage. (a) Chemical...

  10. 33 CFR 110.194 - Mobile Bay, Ala., at entrance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 1 2010-07-01 2010-07-01 false Mobile Bay, Ala., at entrance. 110.194 Section 110.194 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY ANCHORAGES ANCHORAGE REGULATIONS Anchorage Grounds § 110.194 Mobile Bay, Ala., at entrance. (a) The anchorage...

  11. 42 CFR 460.194 - Corrective action.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 4 2011-10-01 2011-10-01 false Corrective action. 460.194 Section 460.194 Public...) Federal/State Monitoring § 460.194 Corrective action. (a) A PACE organization must take action to correct... corrective actions. (c) Failure to correct deficiencies may result in sanctions or termination, as specified...

  12. 42 CFR 460.194 - Corrective action.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Corrective action. 460.194 Section 460.194 Public...) Federal/State Monitoring § 460.194 Corrective action. (a) A PACE organization must take action to correct... corrective actions. (c) Failure to correct deficiencies may result in sanctions or termination, as specified...

  13. Superdeformation in the a Approximately 190 Mass Region and Shape Coexistence in LEAD-194

    NASA Astrophysics Data System (ADS)

    Brinkman, Matthew James

    Near-yrast states in ^{194 }Pb have been identified up to a spin of {~}35hbar following the ^{176}Yb(^ {24}Mg,6n)^{194} Pb^{*} reaction at a beam energy of 134 MeV, measured with the High Energy -Resolution Array located at the Lawrence Berkeley Laboratory 88-Inch Cyclotron facility. Eighteen new transitions were placed. Examples of non-collective prolate and oblate and collective oblate excitations are seen. In addition a rotational band consisting of twelve transitions, with energy spacings characteristic of superdeformed shapes, were also seen. These results have been interpreted using both Nilsson model calculations and previously published potential energy surface calculations. The superdeformed bands in the A ~ 190 mass region are discussed with primary emphasis on ten superdeformed bands in ^{192,193,194 }Hg and ^{192,194,196,198 }Pb discovered or codiscovered by our collaboration. The discussion of superdeformation in these nuclei have been broken into three portions, focusing on the population of, the physics associated with, and the depopulation of these bands, respectively. The population behavior of the superdeformed structures is presented, and discussed with respect to theoretical predictions for nuclei near A ~ 190 expected to support superdeformation. A detailed analysis of the population of the ^{193} Hg^{rm 1a} band is provided, and the results are compared with statistical model calculations predictions. Significant differences were found between the population of the superdeformed bands in the A ~ 150 and 190 mass regions. The systematics of the intraband region are presented. Nilsson model calculations are carried out, with nucleon configurations for the primary superdeformed bands proposed. A discussion of possible mechanisms for reproducing the smooth increase in dynamic moments of inertia observed in all superdeformed bands in this mass region is provided. A number of superdeformed bands in the A ~ 190 mass region have transition energies that are related to those of ^{192}Hg. This behavior is discussed in light of proposed theoretical explanations. The systematic behavior of the depopulation with respect to neutron and proton number is discussed. A comparison of observed depopulation behavior with recently published predictions is provided, showing the predictions in excellent qualitative agreement with the observed depopulation patterns.

  14. 46 CFR 194.05-3 - Chemical stores.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Chemical stores. 194.05-3 Section 194.05-3 Shipping..., AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Stowage and Marking § 194.05-3 Chemical stores. (a) Chemical stores are those chemicals which possess one or more of the following properties and...

  15. 46 CFR 194.05-3 - Chemical stores.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Chemical stores. 194.05-3 Section 194.05-3 Shipping..., AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Stowage and Marking § 194.05-3 Chemical stores. (a) Chemical stores are those chemicals which possess one or more of the following properties and...

  16. 46 CFR 194.05-3 - Chemical stores.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Chemical stores. 194.05-3 Section 194.05-3 Shipping..., AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Stowage and Marking § 194.05-3 Chemical stores. (a) Chemical stores are those chemicals which possess one or more of the following properties and...

  17. 46 CFR 194.05-3 - Chemical stores.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Chemical stores. 194.05-3 Section 194.05-3 Shipping..., AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Stowage and Marking § 194.05-3 Chemical stores. (a) Chemical stores are those chemicals which possess one or more of the following properties and...

  18. 46 CFR 194.05-3 - Chemical stores.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Chemical stores. 194.05-3 Section 194.05-3 Shipping..., AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Stowage and Marking § 194.05-3 Chemical stores. (a) Chemical stores are those chemicals which possess one or more of the following properties and...

  19. 47 CFR 95.194 - (FRS Rule 4) FRS units.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 5 2010-10-01 2010-10-01 false (FRS Rule 4) FRS units. 95.194 Section 95.194 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES PERSONAL RADIO SERVICES Family Radio Service (FRS) General Provisions § 95.194 (FRS Rule 4) FRS units. (a) You may only...

  20. 12 CFR 194.802 - Description of business.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 1 2013-01-01 2013-01-01 false Description of business. 194.802 Section 194... SAVINGS ASSOCIATIONS Interpretations § 194.802 Description of business. (a) This section applies to the description-of-business portion of: (1) Registration statements filed on Form 10 (item 1) (17 CFR 249.210), (2...

  1. 46 CFR 194.10-5 - Type and location.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Type and location. 194.10-5 Section 194.10-5 Shipping..., AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Magazines § 194.10-5 Type and location. (a.... (b) Magazine vans. (1) Magazine vans may be installed on deck in a location protected from boarding...

  2. 1 CFR 19.4 - Proclamations calling for the observance of special days or events.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 1 General Provisions 1 2010-01-01 2010-01-01 false Proclamations calling for the observance of special days or events. 19.4 Section 19.4 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL... PROCLAMATIONS § 19.4 Proclamations calling for the observance of special days or events. Except as may be...

  3. 1 CFR 19.4 - Proclamations calling for the observance of special days or events.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... special days or events. 19.4 Section 19.4 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL... PROCLAMATIONS § 19.4 Proclamations calling for the observance of special days or events. Except as may be... calling for the observance of special days, or other periods of time, or events, shall be assigned by the...

  4. 1 CFR 19.4 - Proclamations calling for the observance of special days or events.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... special days or events. 19.4 Section 19.4 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL... PROCLAMATIONS § 19.4 Proclamations calling for the observance of special days or events. Except as may be... calling for the observance of special days, or other periods of time, or events, shall be assigned by the...

  5. 1 CFR 19.4 - Proclamations calling for the observance of special days or events.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... special days or events. 19.4 Section 19.4 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL... PROCLAMATIONS § 19.4 Proclamations calling for the observance of special days or events. Except as may be... calling for the observance of special days, or other periods of time, or events, shall be assigned by the...

  6. 1 CFR 19.4 - Proclamations calling for the observance of special days or events.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... special days or events. 19.4 Section 19.4 General Provisions ADMINISTRATIVE COMMITTEE OF THE FEDERAL... PROCLAMATIONS § 19.4 Proclamations calling for the observance of special days or events. Except as may be... calling for the observance of special days, or other periods of time, or events, shall be assigned by the...

  7. 15 CFR 19.4 - What notice will Commerce entities send to a debtor when collecting a Commerce debt?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false What notice will Commerce entities send to a debtor when collecting a Commerce debt? 19.4 Section 19.4 Commerce and Foreign Trade Office of the Secretary of Commerce COMMERCE DEBT COLLECTION Procedures To Collect Commerce Debts § 19.4...

  8. 22 CFR 19.4 - Special rules for computing creditable service for purposes of payments to former spouses.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Special rules for computing creditable service for purposes of payments to former spouses. 19.4 Section 19.4 Foreign Relations DEPARTMENT OF STATE... DISABILITY SYSTEM § 19.4 Special rules for computing creditable service for purposes of payments to former...

  9. Engineering Melon Plants with Improved Fruit Shelf Life Using the TILLING Approach

    PubMed Central

    Lévêque, Sylvie; Alsadon, Abdullah A.; Aldoss, Abdullah A.; Dogimont, Catherine; Bendahmane, Abdelhafid

    2010-01-01

    Background Fruit ripening and softening are key traits that have an effect on food supply, fruit nutritional value and consequently, human health. Since ethylene induces ripening of climacteric fruit, it is one of the main targets to control fruit over ripening that leads to fruit softening and deterioration. The characterization of the ethylene pathway in Arabidopsis and tomato identified key genes that control fruit ripening. Methodology/Principal Findings To engineer melon fruit with improved shelf-life, we conducted a translational research experiment. We set up a TILLING platform in a monoecious and climacteric melon line, cloned genes that control ethylene production and screened for induced mutations that lead to fruits with enhanced shelf life. Two missense mutations, L124F and G194D, of the ethylene biosynthetic enzyme, ACC oxidase 1, were identified and the mutant plants were characterized with respect to fruit maturation. The L124F mutation is a conservative mutation occurring away from the enzyme active site and thus was predicted to not affect ethylene production and thus fruit ripening. In contrast, G194D modification occurs in a highly conserved amino acid position predicted, by crystallographic analysis, to affect the enzymatic activity. Phenotypic analysis of the G194D mutant fruit showed complete delayed ripening and yellowing with improved shelf life and, as predicted, the L124F mutation did not have an effect. Conclusions/Significance We constructed a mutant collection of 4023 melon M2 families. Based on the TILLING of 11 genes, we calculated the overall mutation rate of one mutation every 573 kb and identified 8 alleles per tilled kilobase. We also identified a TILLING mutant with enhanced fruit shelf life. This work demonstrates the effectiveness of TILLING as a reverse genetics tool to improve crop species. As cucurbits are model species in different areas of plant biology, we anticipate that the developed tool will be widely exploited by the scientific community. PMID:21209891

  10. Engineering melon plants with improved fruit shelf life using the TILLING approach.

    PubMed

    Dahmani-Mardas, Fatima; Troadec, Christelle; Boualem, Adnane; Lévêque, Sylvie; Alsadon, Abdullah A; Aldoss, Abdullah A; Dogimont, Catherine; Bendahmane, Abdelhafid

    2010-12-30

    Fruit ripening and softening are key traits that have an effect on food supply, fruit nutritional value and consequently, human health. Since ethylene induces ripening of climacteric fruit, it is one of the main targets to control fruit over ripening that leads to fruit softening and deterioration. The characterization of the ethylene pathway in Arabidopsis and tomato identified key genes that control fruit ripening. To engineer melon fruit with improved shelf-life, we conducted a translational research experiment. We set up a TILLING platform in a monoecious and climacteric melon line, cloned genes that control ethylene production and screened for induced mutations that lead to fruits with enhanced shelf life. Two missense mutations, L124F and G194D, of the ethylene biosynthetic enzyme, ACC oxidase 1, were identified and the mutant plants were characterized with respect to fruit maturation. The L124F mutation is a conservative mutation occurring away from the enzyme active site and thus was predicted to not affect ethylene production and thus fruit ripening. In contrast, G194D modification occurs in a highly conserved amino acid position predicted, by crystallographic analysis, to affect the enzymatic activity. Phenotypic analysis of the G194D mutant fruit showed complete delayed ripening and yellowing with improved shelf life and, as predicted, the L124F mutation did not have an effect. We constructed a mutant collection of 4023 melon M2 families. Based on the TILLING of 11 genes, we calculated the overall mutation rate of one mutation every 573 kb and identified 8 alleles per tilled kilobase. We also identified a TILLING mutant with enhanced fruit shelf life. This work demonstrates the effectiveness of TILLING as a reverse genetics tool to improve crop species. As cucurbits are model species in different areas of plant biology, we anticipate that the developed tool will be widely exploited by the scientific community.

  11. Characterization of the functional role of Asp141, Asp194, and Asp464 residues in the Mn2+-L-malate binding of pigeon liver malic enzyme.

    PubMed

    Chou, W Y; Chang, H P; Huang, C H; Kuo, C C; Tong, L; Chang, G G

    2000-02-01

    Pigeon liver malic enzyme was inactivated and cleaved at Asp141, Asp194, and Asp464 by the Cu2+-ascorbate system in acidic environment. Site-specific mutagenesis was performed at these putative metal-binding sites. Three point mutants, D141N, D194N, and D464N; three double mutants, D(141,194)N, D(194,464)N, and D(141,464)N; and a triple mutant, D(141,194,464)N; as well as the wild-type malic enzyme (WT) were successfully cloned and expressed in Escherichia coli cells. All recombinant enzymes, except the triple mutant, were purified to apparent homogeneity by successive Q-Sepharose and adenosine-2',5'-bisphosphate-agarose columns. The mutants showed similar apparent Km,NADP values to that of the WT. The Km,Mal value was increased in the D141N and D194N mutants. The Km,Mn value, on the other hand, was increased only in the D141N mutant by 14-fold, corresponding to approximately 1.6 kcal/mol for the Asp141-Mn2+ binding energy. Substrate inhibition by L-malate was only observed in WT, D464N, and D(141,464)N. Initial velocity experiments were performed to derive the various kinetic parameters. The possible interactions between Asp141, Asp194, and Asp464 were analyzed by the double-mutation cycles and triple-mutation box. There are synergistic weakening interactions between Asp141 and Asp194 in the metal binding that impel the D(141,194)N double mutant to an overall specificity constant [k(cat)/(Kd,Mn Km,Mal Km,NADP)] at least four orders of magnitude smaller than the WT value. This difference corresponds to an increase of 6.38 kcal/mol energy barrier for the catalytic efficiency. Mutation at Asp464, on the other hand, has partial additivity on the mutations at Asp141 and Asp194. The overall specificity constants for the double mutants D(194,464)N and D(141,464)N or the triple mutant D(141,194,464)N were decreased by only 10- to 100-fold compared to the WT. These results strongly suggest the involvement of Asp141 in the Mn2+-L-malate binding for the pigeon liver malic enzyme. The Asp194 and Asp464, which may be oxidized by nonspecific binding of Cu2+, are involved in the Mn2+-L-malate binding or catalysis indirectly by modulating the binding affinity of Asp141 with the Mn2+.

  12. Overexpression of microRNA-194 suppresses the epithelial-mesenchymal transition in targeting stem cell transcription factor Sox3 in endometrial carcinoma stem cells.

    PubMed

    Gong, Baolan; Yue, Yan; Wang, Renxiao; Zhang, Yi; Jin, Quanfang; Zhou, Xi

    2017-06-01

    The epithelial-mesenchymal transition is the key process driving cancer metastasis. MicroRNA-194 inhibits epithelial-mesenchymal transition in several cancers and its downregulation indicates a poor prognosis in human endometrial carcinoma. Self-renewal factor Sox3 induces epithelial-mesenchymal transition at gastrulation and is also involved epithelial-mesenchymal transition in several cancers. We intended to determine the roles of Sox3 in inducing epithelial-mesenchymal transition in endometrial cancer stem cells and the possible role of microRNA-194 in controlling Sox3 expression. Firstly, we found that Sox3 and microRNA-194 expressions were associated with the status of endometrial cancer stem cells in a panel of endometrial carcinoma tissue, the CD133+ cell was higher in tumorsphere than in differentiated cells, and overexpression of microRNA-194 would decrease CD133+ cell expression. Silencing of Sox3 in endometrial cancer stem cell upregulated the epithelial marker E-cadherin, downregulated the mesenchymal marker vimentin, and significantly reduced cell invasion in vitro; overexpression of Sox3 reversed these phenotypes. Furthermore, we discovered that the expression of Sox3 was suppressed by microRNA-194 through direct binding to the Sox3 3'-untranslated region. Ectopic expression of microRNA-194 in endometrial cancer stem cells induced a mesenchymal-epithelial transition by restoring E-cadherin expression, decreasing vimentin expression, and inhibiting cell invasion in vitro. Moreover, overexpression of microRNA-194 inhibited endometrial cancer stem cell invasion or metastasis in vivo by injection of adenovirus microRNA-194. These findings demonstrate the novel mechanism by which Sox3 contributes to endometrial cancer stem cell invasion and suggest that repression of Sox3 by microRNA-194 may have therapeutic potential to suppress endometrial carcinoma metastasis. The cancer stem cell marker, CD133, might be the surface marker of endometrial cancer stem cell.

  13. Unraveling the Deleterious Effects of Cancer-Driven STK11 Mutants Through Conformational Sampling Approach.

    PubMed

    Lopus, Merlin; Paul, D Meshach; Rajasekaran, R

    2016-01-01

    Tumor suppressor gene, STK11, encodes for serine-threonine kinase, which has a critical role in regulating cell growth and apoptosis. Mutations of the same lead to the inactivation of STK11, which eventually causes different types of cancer. In this study, we focused on identifying those driver mutations through analyzing structural variations of mutants, viz., D194N, E199K, L160P, and Y49D. Native and the mutants were analyzed to determine their geometrical deviations such as root-mean-square deviation, root-mean-square fluctuation, radius of gyration, potential energy, and solvent-accessible surface area using conformational sampling technique. Additionally, the global minimized structure of native and mutants was further analyzed to compute their intramolecular interactions and distribution of secondary structure. Subsequently, simulated thermal denaturation and docking studies were performed to determine their structural variations, which in turn alter the formation of active complex that comprises STK11, STRAD, and MO25. The deleterious effect of the mutants would result in a comparative loss of enzyme function due to variations in their binding energy pertaining to spatial conformation and flexibility. Hence, the structural variations in binding energy exhibited by the mutants, viz., D194N, E199K, L160P, and Y49D, to that of the native, consequently lead to pathogenesis.

  14. 34 CFR 668.194 - Economically disadvantaged appeals.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 3 2010-07-01 2010-07-01 false Economically disadvantaged appeals. 668.194 Section 668.194 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION STUDENT ASSISTANCE GENERAL PROVISIONS Two Year Cohort Default...

  15. 40 CFR 194.6 - Alternative provisions.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Alternative provisions. 194.6 Section... COMPLIANCE WITH THE 40 CFR PART 191 DISPOSAL REGULATIONS General Provisions § 194.6 Alternative provisions... part alternative provisions, or minor alternative provisions, in accordance with the following...

  16. 25 CFR 900.194 - Does FTCA coverage extend to services provided under a staff privileges agreement with a non-IHS...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... practitioner to provide reciprocal services to the general population? 900.194 Section 900.194 Indians BUREAU... health care practitioner to provide reciprocal services to the general population? Yes. Those services...

  17. 25 CFR 900.194 - Does FTCA coverage extend to services provided under a staff privileges agreement with a non-IHS...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... practitioner to provide reciprocal services to the general population? 900.194 Section 900.194 Indians BUREAU... health care practitioner to provide reciprocal services to the general population? Yes. Those services...

  18. 25 CFR 900.194 - Does FTCA coverage extend to services provided under a staff privileges agreement with a non-IHS...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... practitioner to provide reciprocal services to the general population? 900.194 Section 900.194 Indians BUREAU... health care practitioner to provide reciprocal services to the general population? Yes. Those services...

  19. 25 CFR 900.194 - Does FTCA coverage extend to services provided under a staff privileges agreement with a non-IHS...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... practitioner to provide reciprocal services to the general population? 900.194 Section 900.194 Indians BUREAU... health care practitioner to provide reciprocal services to the general population? Yes. Those services...

  20. 25 CFR 900.194 - Does FTCA coverage extend to services provided under a staff privileges agreement with a non-IHS...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... practitioner to provide reciprocal services to the general population? 900.194 Section 900.194 Indians BUREAU... health care practitioner to provide reciprocal services to the general population? Yes. Those services...

  1. 40 CFR 194.46 - Removal of waste.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Removal of waste. 194.46 Section 194.46 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION PROTECTION... time after disposal. Such documentation shall include an analysis of the technological feasibility of...

  2. 40 CFR 194.46 - Removal of waste.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Removal of waste. 194.46 Section 194.46 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION PROTECTION... time after disposal. Such documentation shall include an analysis of the technological feasibility of...

  3. 40 CFR 194.46 - Removal of waste.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Removal of waste. 194.46 Section 194.46 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION PROTECTION... time after disposal. Such documentation shall include an analysis of the technological feasibility of...

  4. 40 CFR 194.46 - Removal of waste.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Removal of waste. 194.46 Section 194.46 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION PROTECTION... time after disposal. Such documentation shall include an analysis of the technological feasibility of...

  5. 40 CFR 194.41 - Active institutional controls.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Active institutional controls. 194.41... Assurance Requirements § 194.41 Active institutional controls. (a) Any compliance application shall include detailed descriptions of proposed active institutional controls, the controls' location, and the period of...

  6. 40 CFR 194.41 - Active institutional controls.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Active institutional controls. 194.41... Assurance Requirements § 194.41 Active institutional controls. (a) Any compliance application shall include detailed descriptions of proposed active institutional controls, the controls' location, and the period of...

  7. 40 CFR 194.41 - Active institutional controls.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Active institutional controls. 194.41... Assurance Requirements § 194.41 Active institutional controls. (a) Any compliance application shall include detailed descriptions of proposed active institutional controls, the controls' location, and the period of...

  8. 40 CFR 194.41 - Active institutional controls.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Active institutional controls. 194.41... Assurance Requirements § 194.41 Active institutional controls. (a) Any compliance application shall include detailed descriptions of proposed active institutional controls, the controls' location, and the period of...

  9. 40 CFR 194.41 - Active institutional controls.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Active institutional controls. 194.41... Assurance Requirements § 194.41 Active institutional controls. (a) Any compliance application shall include detailed descriptions of proposed active institutional controls, the controls' location, and the period of...

  10. 46 CFR 194.15-11 - Flushing systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Flushing systems. 194.15-11 Section 194.15-11 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory...

  11. 46 CFR 194.15-7 - Fire protection.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Fire protection. 194.15-7 Section 194.15-7 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory...

  12. 46 CFR 194.15-7 - Fire protection.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Fire protection. 194.15-7 Section 194.15-7 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory...

  13. 46 CFR 194.15-11 - Flushing systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Flushing systems. 194.15-11 Section 194.15-11 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory...

  14. 40 CFR 194.46 - Removal of waste.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Removal of waste. 194.46 Section 194.46 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION PROTECTION PROGRAMS CRITERIA... disposal. Such documentation shall include an analysis of the technological feasibility of mining the...

  15. 46 CFR 194.90-1 - Requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Requirements. 194.90-1 Section 194.90-1 Shipping COAST...-1 Requirements. (a) Vessels contracted for prior to March 1, 1968, shall meet the following requirements: (1) Existing arrangements, materials, and facilities previously approved but not meeting the...

  16. 46 CFR 194.90-1 - Requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Requirements. 194.90-1 Section 194.90-1 Shipping COAST...-1 Requirements. (a) Vessels contracted for prior to March 1, 1968, shall meet the following requirements: (1) Existing arrangements, materials, and facilities previously approved but not meeting the...

  17. 46 CFR 194.90-1 - Requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Requirements. 194.90-1 Section 194.90-1 Shipping COAST...-1 Requirements. (a) Vessels contracted for prior to March 1, 1968, shall meet the following requirements: (1) Existing arrangements, materials, and facilities previously approved but not meeting the...

  18. 46 CFR 194.90-1 - Requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Requirements. 194.90-1 Section 194.90-1 Shipping COAST...-1 Requirements. (a) Vessels contracted for prior to March 1, 1968, shall meet the following requirements: (1) Existing arrangements, materials, and facilities previously approved but not meeting the...

  19. 46 CFR 194.20-11 - Flushing systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Flushing systems. 194.20-11 Section 194.20-11 Shipping... Flushing systems. (a) Provision shall be made for flushing away chemical spills. (b) If a drainage system is installed, it shall be separate from any other drainage system. ...

  20. 46 CFR 194.20-11 - Flushing systems.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Flushing systems. 194.20-11 Section 194.20-11 Shipping... Flushing systems. (a) Provision shall be made for flushing away chemical spills. (b) If a drainage system is installed, it shall be separate from any other drainage system. ...

  1. 40 CFR 194.52 - Consideration of exposure pathways.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Consideration of exposure pathways. 194... Individual and Ground-Water Protection Requirements § 194.52 Consideration of exposure pathways. In compliance assessments that analyze compliance with § 191.15 of this chapter, all potential exposure pathways...

  2. 40 CFR 194.33 - Consideration of drilling events in performance assessments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Consideration of drilling events in performance assessments. 194.33 Section 194.33 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... assessments. (a) Performance assessments shall examine deep drilling and shallow drilling that may potentially...

  3. 40 CFR 194.14 - Content of compliance certification application.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Content of compliance certification application. 194.14 Section 194.14 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED... shall include: (a) A current description of the natural and engineered features that may affect the...

  4. 46 CFR 194.20-11 - Flushing systems.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Flushing systems. 194.20-11 Section 194.20-11 Shipping... Flushing systems. (a) Provision shall be made for flushing away chemical spills. (b) If a drainage system is installed, it shall be separate from any other drainage system. ...

  5. 37 CFR 2.194 - Identification of trademark application or registration.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... applicant, and the mark. (2) A letter about a registered trademark should identify the registration number... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Identification of trademark application or registration. 2.194 Section 2.194 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND...

  6. 46 CFR 194.10-15 - Magazine van construction.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Magazine van construction. 194.10-15 Section 194.10-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING... system shall be of watertight construction and bear a label plate denoting the power requirement of the...

  7. 46 CFR 194.05-11 - Flammable solids and oxidizing materials-Detail requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Flammable solids and oxidizing materials-Detail... and Marking § 194.05-11 Flammable solids and oxidizing materials—Detail requirements. (a) Flammable... 194.20. (b) Oxidizing materials used as blasting agents are regulated by the appropriate portions of...

  8. 46 CFR 194.05-11 - Flammable solids and oxidizing materials-Detail requirements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Flammable solids and oxidizing materials-Detail... and Marking § 194.05-11 Flammable solids and oxidizing materials—Detail requirements. (a) Flammable... 194.20. (b) Oxidizing materials used as blasting agents are regulated by the appropriate portions of...

  9. 46 CFR 194.05-11 - Flammable solids and oxidizing materials-Detail requirements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Flammable solids and oxidizing materials-Detail... and Marking § 194.05-11 Flammable solids and oxidizing materials—Detail requirements. (a) Flammable... 194.20. (b) Oxidizing materials used as blasting agents are regulated by the appropriate portions of...

  10. 46 CFR 194.15-17 - Compressed gases other than inert gases.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Compressed gases other than inert gases. 194.15-17 Section 194.15-17 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and...

  11. 46 CFR 194.15-15 - Chemicals other than compressed gases.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Chemicals other than compressed gases. 194.15-15 Section 194.15-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and...

  12. 40 CFR 194.44 - Engineered barriers.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... prevent or substantially delay the movement of water or waste toward the accessible environment; (ii) The... reduced total system costs; (viii) The impact, if any, on other waste disposal programs from the... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Engineered barriers. 194.44 Section 194...

  13. 41 CFR 102-194.25 - What is an automated Standard or Optional format?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION ADMINISTRATIVE PROGRAMS 194-STANDARD AND OPTIONAL FORMS MANAGEMENT PROGRAM § 102-194.25 What is an automated Standard or... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false What is an automated...

  14. 46 CFR 194.15-17 - Compressed gases other than inert gases.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Compressed gases other than inert gases. 194.15-17 Section 194.15-17 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and...

  15. 46 CFR 194.15-15 - Chemicals other than compressed gases.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Chemicals other than compressed gases. 194.15-15 Section 194.15-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and...

  16. 46 CFR 194.20-7 - Fire protection.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Fire protection. 194.20-7 Section 194.20-7 Shipping... Fire protection. (a) Each chemical storeroom shall be protected by a fixed automatic carbon dioxide... fire extinguishers are required in accordance with Table 193.50-10(a) of this subchapter. ...

  17. 40 CFR 194.23 - Models and computer codes.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Models and computer codes. 194.23... General Requirements § 194.23 Models and computer codes. (a) Any compliance application shall include: (1... obtain stable solutions; (iv) Computer models accurately implement the numerical models; i.e., computer...

  18. 40 CFR 194.23 - Models and computer codes.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Models and computer codes. 194.23... General Requirements § 194.23 Models and computer codes. (a) Any compliance application shall include: (1... obtain stable solutions; (iv) Computer models accurately implement the numerical models; i.e., computer...

  19. 40 CFR 194.23 - Models and computer codes.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Models and computer codes. 194.23... General Requirements § 194.23 Models and computer codes. (a) Any compliance application shall include: (1... obtain stable solutions; (iv) Computer models accurately implement the numerical models; i.e., computer...

  20. 40 CFR 194.23 - Models and computer codes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Models and computer codes. 194.23... General Requirements § 194.23 Models and computer codes. (a) Any compliance application shall include: (1... obtain stable solutions; (iv) Computer models accurately implement the numerical models; i.e., computer...

  1. 40 CFR 194.23 - Models and computer codes.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Models and computer codes. 194.23... General Requirements § 194.23 Models and computer codes. (a) Any compliance application shall include: (1... obtain stable solutions; (iv) Computer models accurately implement the numerical models; i.e., computer...

  2. Reflex anoxic seizures ('white breath-holding'): nonepileptic vagal attacks.

    PubMed Central

    Stephenson, J B

    1978-01-01

    From clinical history 58 children were diagnosed as having reflex anoxic seizures secondary to provoked cardioinhibition (also known as white breath-holding attacks). Before referral, these seizures were commonly misdiagnosed as epileptic either because the provocation was ignored, not recognised, or was a febrile illness, or because there was no crying, no obvious breath-holding, little cyanosis, and often no pallor to suggest syncope and cerebral ischaemia. The duration of cardiac asystole after ocular compression was measured in these children and in 60 additional children with other paroxysmal disorders. In 45 (78%) of the 58 with reflex anoxic seizures asystole was 2 seconds or over, and in 32 (55%) it was 4 seconds or greater, an abnormal response. Review of the literature supports the concept that these seizures result from vagal-mediated reflex cardiac arrest which can if necessary be prevented by atropine. The simple name 'vagal attack' is proposed. Ocular compression under EEG and ECG control supports the clinical diagnosis if asystole and/or an anoxic seizure is induced; the procedure described is safe and should be routine in seizure or syncope evaluation, when a meticulous history still leaves room for doubt. Images Figs. 1-8 p194-b p194-c p194-d p194-e p194-f p194-g p194-h PMID:348123

  3. Acquisition Program Teamwork and Performance Seen Anew: Exposing the Interplay of Architecture and Behaviors in Complex Defense Programs

    DTIC Science & Technology

    2016-04-30

    fåÑçêãÉÇ=`Ü~åÖÉ= - 194 - Panel 16. Improving Governance of Complex Systems Acquisition Thursday, May 5, 2016 11:15 a.m. – 12:45 p.m. Chair: Rear...Admiral David Gale, USN, Program Executive Officer, SHIPS Complex System Governance for Acquisition Joseph Bradley, President, Leading Change, LLC...Bryan Moser, Lecturer, MIT John Dickmann, Vice President, Sonalysts Inc. A Complex Systems Perspective of Risk Mitigation and Modeling in

  4. Photocatalytic mineralization and degradation kinetics of sulphamethoxazole and reactive red 194 over silver-zirconium co-doped titanium dioxide: Reaction mechanisms and phytotoxicity assessment.

    PubMed

    Naraginti, Saraschandra; Li, Yi; Puma, Gianluca Li

    2018-09-15

    The photodegradation and phytotoxicity of the pharmaceutical antibiotic, sulphamethoxazole (SMX) and the azo-dye reactive-red-194 (RR194) under visible-light irradiation of TiO 2 nanoparticles modified by silver and zirconium was investigated. The results indicated that sulphamethoxazole and its toxic degradation by product, 3-amino-5-methylisoxazole and RR-194 could be degraded efficiently by the co-doped Zr/Ag-TiO 2 catalyst. PL studies and ROS generation results suggested that the effective charge separation was carried out while irradiation of the modified TiO 2 nanoparticles. Phytotoxicity tests demonstrated lower percentage of germination in P. vulgaris (40%), V. radiata (30%) and P. lunatus (30%) of the seeds treated with 50 ppm of SMX, compared to the seeds treated with the degradation products (100%). The results with 50 ppm of RR-194 also showed lower percentage of germination in P. vulgaris (40%), V. radiata (50%) and P. lunatus (30%) compared to the degradation products (100%). Furthermore, significant increase in root and shoot development was observed in the seeds treated with the degraded products when compared with SMX and RR-194. Overall, this study contributes to further understanding the photodegradation mechanisms, degradation products and environmental fate of SMX and RR-194 in water which helps in the evaluation and mitigation of the environmental risk of SMX and RR-194 for water reuse and crop irrigation. Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.

  5. 46 CFR 194.15-15 - Chemicals other than compressed gases.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Chemicals other than compressed gases. 194.15-15 Section... Scientific Laboratory § 194.15-15 Chemicals other than compressed gases. Chemicals, including those listed in 49 CFR part 172, may be stored in small working quantities in the chemical laboratory. [CGD 86-033...

  6. 49 CFR 194.121 - Response plan review and update procedures.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 49 Transportation 3 2011-10-01 2011-10-01 false Response plan review and update procedures. 194... SAFETY RESPONSE PLANS FOR ONSHORE OIL PIPELINES Response Plans § 194.121 Response plan review and update procedures. (a) Each operator shall update its response plan to address new or different operating conditions...

  7. 49 CFR 194.121 - Response plan review and update procedures.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 49 Transportation 3 2013-10-01 2013-10-01 false Response plan review and update procedures. 194... SAFETY RESPONSE PLANS FOR ONSHORE OIL PIPELINES Response Plans § 194.121 Response plan review and update procedures. (a) Each operator shall update its response plan to address new or different operating conditions...

  8. 49 CFR 194.121 - Response plan review and update procedures.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 49 Transportation 3 2014-10-01 2014-10-01 false Response plan review and update procedures. 194... SAFETY RESPONSE PLANS FOR ONSHORE OIL PIPELINES Response Plans § 194.121 Response plan review and update procedures. (a) Each operator shall update its response plan to address new or different operating conditions...

  9. 49 CFR 194.121 - Response plan review and update procedures.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 49 Transportation 3 2012-10-01 2012-10-01 false Response plan review and update procedures. 194... SAFETY RESPONSE PLANS FOR ONSHORE OIL PIPELINES Response Plans § 194.121 Response plan review and update procedures. (a) Each operator shall update its response plan to address new or different operating conditions...

  10. 49 CFR 194.121 - Response plan review and update procedures.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 49 Transportation 3 2010-10-01 2010-10-01 false Response plan review and update procedures. 194... SAFETY RESPONSE PLANS FOR ONSHORE OIL PIPELINES Response Plans § 194.121 Response plan review and update procedures. (a) Each operator shall update its response plan to address new or different operating conditions...

  11. 22 CFR Appendix A to Part 194 - Inter-American Commercial Arbitration Commission Rules of Procedure (As Amended April 1, 2002)

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Inter-American Commercial Arbitration... DEPARTMENT OF STATE INTERNATIONAL COMMERCIAL ARBITRATION INTER-AMERICAN COMMERCIAL ARBITRATION COMMISSION RULES OF PROCEDURE Pt. 194, App. A Appendix A to Part 194—Inter-American Commercial Arbitration...

  12. 22 CFR Appendix A to Part 194 - Inter-American Commercial Arbitration Commission Rules of Procedure (As Amended April 1, 2002)

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Inter-American Commercial Arbitration... DEPARTMENT OF STATE INTERNATIONAL COMMERCIAL ARBITRATION INTER-AMERICAN COMMERCIAL ARBITRATION COMMISSION RULES OF PROCEDURE Pt. 194, App. A Appendix A to Part 194—Inter-American Commercial Arbitration...

  13. 22 CFR Appendix A to Part 194 - Inter-American Commercial Arbitration Commission Rules of Procedure (As Amended April 1, 2002)

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Inter-American Commercial Arbitration... DEPARTMENT OF STATE INTERNATIONAL COMMERCIAL ARBITRATION INTER-AMERICAN COMMERCIAL ARBITRATION COMMISSION RULES OF PROCEDURE Pt. 194, App. A Appendix A to Part 194—Inter-American Commercial Arbitration...

  14. 22 CFR Appendix A to Part 194 - Inter-American Commercial Arbitration Commission Rules of Procedure (As Amended April 1, 2002)

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Inter-American Commercial Arbitration... DEPARTMENT OF STATE INTERNATIONAL COMMERCIAL ARBITRATION INTER-AMERICAN COMMERCIAL ARBITRATION COMMISSION RULES OF PROCEDURE Pt. 194, App. A Appendix A to Part 194—Inter-American Commercial Arbitration...

  15. 41 CFR 102-194.40 - For what Standard or Optional forms should an electronic version not be made available?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 41 Public Contracts and Property Management 3 2010-07-01 2010-07-01 false For what Standard or... Contracts and Property Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION ADMINISTRATIVE PROGRAMS 194-STANDARD AND OPTIONAL FORMS MANAGEMENT PROGRAM § 102-194.40...

  16. 46 CFR 194.15-15 - Chemicals other than compressed gases.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Chemicals other than compressed gases. 194.15-15 Section... Scientific Laboratory § 194.15-15 Chemicals other than compressed gases. Chemicals, including those listed in 49 CFR part 172, may be stored in small working quantities in the chemical laboratory. [CGD 86-033...

  17. 46 CFR 194.15-15 - Chemicals other than compressed gases.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Chemicals other than compressed gases. 194.15-15 Section... Scientific Laboratory § 194.15-15 Chemicals other than compressed gases. Chemicals, including those listed in 49 CFR part 172, may be stored in small working quantities in the chemical laboratory. [CGD 86-033...

  18. 13 CFR 120.194 - Use of computer forms.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Use of computer forms. 120.194... Applying to All Business Loans Computerized Sba Forms § 120.194 Use of computer forms. Any Applicant or Participant may use computer generated SBA application forms, closing forms, and other forms designated by SBA...

  19. 13 CFR 120.194 - Use of computer forms.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Use of computer forms. 120.194... Applying to All Business Loans Computerized Sba Forms § 120.194 Use of computer forms. Any Applicant or Participant may use computer generated SBA application forms, closing forms, and other forms designated by SBA...

  20. 13 CFR 120.194 - Use of computer forms.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Use of computer forms. 120.194... Applying to All Business Loans Computerized Sba Forms § 120.194 Use of computer forms. Any Applicant or Participant may use computer generated SBA application forms, closing forms, and other forms designated by SBA...

  1. 40 CFR 194.53 - Consideration of underground sources of drinking water.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Consideration of underground sources of drinking water. 194.53 Section 194.53 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION PROTECTION PROGRAMS CRITERIA FOR THE CERTIFICATION AND RE-CERTIFICATION OF THE WASTE ISOLATION PILOT PLANT'S COMPLIANCE WITH THE...

  2. 40 CFR 194.53 - Consideration of underground sources of drinking water.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Consideration of underground sources of drinking water. 194.53 Section 194.53 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) RADIATION PROTECTION PROGRAMS CRITERIA FOR THE CERTIFICATION AND RE-CERTIFICATION OF THE WASTE ISOLATION PILOT PLANT'S COMPLIANCE WITH THE...

  3. 20 CFR 1002.194 - Can the application of the escalator principle result in adverse consequences when the employee...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... principle result in adverse consequences when the employee is reemployed? 1002.194 Section 1002.194... principle result in adverse consequences when the employee is reemployed? Yes. The Act does not prohibit lawful adverse job consequences that result from the employee's restoration on the seniority ladder...

  4. 46 CFR 194.05-11 - Flammable solids and oxidizing materials-Detail requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Flammable solids and oxidizing materials-Detail... and Marking § 194.05-11 Flammable solids and oxidizing materials—Detail requirements. (a) Flammable solids and oxidizing materials used as chemical stores and reagents are governed by subparts 194.15 and...

  5. 46 CFR 194.05-11 - Flammable solids and oxidizing materials-Detail requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Flammable solids and oxidizing materials-Detail... and Marking § 194.05-11 Flammable solids and oxidizing materials—Detail requirements. (a) Flammable solids and oxidizing materials used as chemical stores and reagents are governed by subparts 194.15 and...

  6. 13 CFR 120.194 - Use of computer forms.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Use of computer forms. 120.194... Applying to All Business Loans Computerized Sba Forms § 120.194 Use of computer forms. Any Applicant or Participant may use computer generated SBA application forms, closing forms, and other forms designated by SBA...

  7. 13 CFR 120.194 - Use of computer forms.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Use of computer forms. 120.194... Applying to All Business Loans Computerized Sba Forms § 120.194 Use of computer forms. Any Applicant or Participant may use computer generated SBA application forms, closing forms, and other forms designated by SBA...

  8. 12 CFR 19.4 - Authority of the Comptroller.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Authority of the Comptroller. 19.4 Section 19.4 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY RULES OF PRACTICE AND..., at any time during the pendency of a proceeding, perform, direct the performance of, or waive...

  9. Long noncoding RNA SOX2OT contributes to gastric cancer progression by sponging miR-194-5p from AKT2.

    PubMed

    Qu, Fei; Cao, Peilong

    2018-05-18

    Gastric cancer (GC) is a highly malignant cancer with poor prognosis. Long non-coding RNA (LncRNA) may play an important role in tumor progression. Our present study aimed to explore the effect of LncRNA SOX2OT on GC progression. We observed that SOX2OT was overexpressed in GC tissues and cell lines. Overexpressed SOX2OT promoted cell proliferation and metastasis of GC cells (SGC-7901, TMK-1) and the phosphorylation of AKT2 as well, while knockdown of SOX2OT reversed these effects. Besides that, miR-194-5p was predicted to be a target of SOX2OT and decreased expression of miR-194-5p was observed in GC tissues and cell lines. Overexpressed miR-194-5p counteracted the promoting role of SOX2OT on cell proliferation and invasion of GC cells. Moreover, AKT2 was predicted to be a target of miR-194-5p. The expression of AKT2 was negatively regulated by miR-194-5p while positively regulated by SOX2OT. Overexpressed AKT2 also promoted GC cell proliferation and invasion. Our in vitro experiments suggested that SOX2OT promoted cell proliferation and metastasis of GC cells via sponging miR-194-5p from AKT2. Finally, our in vivo experiments indicated that overexpressed SOX2OT promoted GC tumor growth and metastasis in nude mice. Taken together, our present study suggested that SOX2OT contributed to GC progression via sponging miR-194-5p from AKT2 both in vitro and in vivo. The SOX2OT-miR-194-5p-AKT2 axis may provide a new perspective for treatment of GC. Copyright © 2018. Published by Elsevier Inc.

  10. Dielectric Constant and Loss Data. Part 4

    DTIC Science & Technology

    1980-12-01

    Fluorinated ethers, Organic Chemicals Dept., P.R.-194Dow Chemical "Dowtherm" A, P.R.-194 T ORg C a p - Dew Corning Corp., IV-26, 27, 41, 42, "HVITON...Esso "Teresso" oil, V-78; P.R.-195 and MFl16, V-15 "Estawax", IV-57 "Eccosorb" MFll7, V-15, 242 Ethers, fluorinated , P.R.-194 Eggwhite. P.R.-202 "Ethocel...PPG, 8-20 Flourglas laminate, 9-33 "Gafite" cast polymer, IV-34 Fluorinated ethers, P.R.-194 Gasoline, aviation, 100 and 91 octane, Fluorcarbon

  11. Historical review of the List of Occupational Diseases recommended by the International Labour organization (ILO)

    PubMed Central

    2013-01-01

    The list of occupational diseases established in the international and national legal system has played important roles in both prevention of and compensation for workers’ diseases. This report reviewed the historical development in the ILO list of occupational diseases and suggested implications of the trends. Since the first establishment of the ILO list of occupational diseases in 1925, the list has played a key role in harmonizing the development of policies on occupational diseases at the international level. The three occupational diseases (anthrax, lead poisoning, and mercury poisoning) in the first ILO list of occupational diseases, set up in 1925 as workmen’s compensation convention represented an increase of occupational diseases from the Industrial Revolution. Until the 1960s, 10 occupational diseases had been representative compensable occupational diseases listed in Convention No. 121, which implies that occupational diseases in this era were equated to industrial poisoning. Since 1980, with advancements in diagnostic techniques and medical science, noise-induced hearing loss, and several bronchopulmonary diseases have been incorporated into the ILO occupational list. Since 2002, changes in the structure of industries, emerging new chemicals, and advanced national worker’s compensation schemes have provoked the ILO to revise the occupational disease list. A new format of ILO list appended in Recommendation 194 (R194) was composed of two dimensions (causes and diseases) and subcategories. Among 50 member states that had provided their national lists of occupational diseases, until 2012 thirty countries were found to have the list occupational diseases having similar structure to ILO list in R194. PMID:24472440

  12. 49 CFR Appendix A to Part 194 - Guidelines for the Preparation of Response Plans

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) Oil spill removal organization field equipment deployment drills conducted yearly. (5) A drill that... PLANS FOR ONSHORE OIL PIPELINES Pt. 194, App. A Appendix A to Part 194—Guidelines for the Preparation of... substantial harm to the environment; (4) A list of line sections contained in the response zone, identified by...

  13. 49 CFR Appendix A to Part 194 - Guidelines for the Preparation of Response Plans

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...) Oil spill removal organization field equipment deployment drills conducted yearly. (5) A drill that... PLANS FOR ONSHORE OIL PIPELINES Pt. 194, App. A Appendix A to Part 194—Guidelines for the Preparation of... substantial harm to the environment; (4) A list of line sections contained in the response zone, identified by...

  14. 49 CFR Appendix A to Part 194 - Guidelines for the Preparation of Response Plans

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...) Oil spill removal organization field equipment deployment drills conducted yearly. (5) A drill that... PLANS FOR ONSHORE OIL PIPELINES Pt. 194, App. A Appendix A to Part 194—Guidelines for the Preparation of... substantial harm to the environment; (4) A list of line sections contained in the response zone, identified by...

  15. 49 CFR Appendix A to Part 194 - Guidelines for the Preparation of Response Plans

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...) Oil spill removal organization field equipment deployment drills conducted yearly. (5) A drill that... PLANS FOR ONSHORE OIL PIPELINES Pt. 194, App. A Appendix A to Part 194—Guidelines for the Preparation of... substantial harm to the environment; (4) A list of line sections contained in the response zone, identified by...

  16. 49 CFR Appendix A to Part 194 - Guidelines for the Preparation of Response Plans

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...) Oil spill removal organization field equipment deployment drills conducted yearly. (5) A drill that... PLANS FOR ONSHORE OIL PIPELINES Pt. 194, App. A Appendix A to Part 194—Guidelines for the Preparation of... substantial harm to the environment; (4) A list of line sections contained in the response zone, identified by...

  17. 34 CFR Appendix B to Part 403 - Examples for 34 CFR 403.194-Comparability Requirements

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... TECHNOLOGY EDUCATION PROGRAM Pt. 403, App. B Appendix B to Part 403—Examples for 34 CFR 403.194—Comparability Requirements Methods by which a local educational agency can demonstrate its compliance with the comparability requirements in 34 CFR 403.194(a) include the following: Example 1: The local educational agency files with the...

  18. 33 CFR 110.194b - Mississippi Sound and Gulf of Mexico, near Petit Bois Island, Miss.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Island. (2) Explosives Anchorage Area No. 2. A circular area with a three-fourths mile radius with its... Mexico, near Petit Bois Island, Miss. 110.194b Section 110.194b Navigation and Navigable Waters COAST... Mississippi Sound and Gulf of Mexico, near Petit Bois Island, Miss. (a) The anchorage grounds—(1) Explosives...

  19. 33 CFR 110.194b - Mississippi Sound and Gulf of Mexico, near Petit Bois Island, Miss.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Island. (2) Explosives Anchorage Area No. 2. A circular area with a three-fourths mile radius with its... Mexico, near Petit Bois Island, Miss. 110.194b Section 110.194b Navigation and Navigable Waters COAST... Mississippi Sound and Gulf of Mexico, near Petit Bois Island, Miss. (a) The anchorage grounds—(1) Explosives...

  20. 33 CFR 110.194b - Mississippi Sound and Gulf of Mexico, near Petit Bois Island, Miss.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Island. (2) Explosives Anchorage Area No. 2. A circular area with a three-fourths mile radius with its... Mexico, near Petit Bois Island, Miss. 110.194b Section 110.194b Navigation and Navigable Waters COAST... Mississippi Sound and Gulf of Mexico, near Petit Bois Island, Miss. (a) The anchorage grounds—(1) Explosives...

  1. 33 CFR 110.194b - Mississippi Sound and Gulf of Mexico, near Petit Bois Island, Miss.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Island. (2) Explosives Anchorage Area No. 2. A circular area with a three-fourths mile radius with its... Mexico, near Petit Bois Island, Miss. 110.194b Section 110.194b Navigation and Navigable Waters COAST... Mississippi Sound and Gulf of Mexico, near Petit Bois Island, Miss. (a) The anchorage grounds—(1) Explosives...

  2. Specific binding of Clostridium perfringens enterotoxin fragment to Claudin-b and modulation of zebrafish epidermal barrier.

    PubMed

    Zhang, Jingjing; Ni, Chen; Yang, Zhenguo; Piontek, Anna; Chen, Huapu; Wang, Sijie; Fan, Yiming; Qin, Zhihai; Piontek, Joerg

    2015-08-01

    Claudins (Cldn) are the major components of tight junctions (TJs) sealing the paracellular cleft in tissue barriers of various organs. Zebrafish Cldnb, the homolog of mammalian Cldn4, is expressed at epithelial cell-cell contacts and is important for regulating epidermal permeability. The bacterial toxin Clostridium perfringens enterotoxin (CPE) has been shown to bind to a subset of mammalian Cldns. In this study, we used the Cldn-binding C-terminal domain of CPE (194-319 amino acids, cCPE 194-319 ) to investigate its functional role in modulating zebrafish larval epidermal barriers. In vitro analyses show that cCPE 194-319 removed Cldn4 from epithelial cells and disrupted the monolayer tightness, which could be rescued by the removal of cCPE 194-319. Incubation of zebrafish larvae with cCPE 194-319 removed Cldnb specifically from the epidermal cell membrane. Dye diffusion analysis with 4-kDa fluorescent dextran indicated that the permeability of the epidermal barrier increased due to cCPE 194-319 incubation. Electron microscopic investigation revealed reversible loss of TJ integrity by Cldnb removal. Collectively, these results suggest that cCPE 194-319 could be used as a Cldnb modulator to transiently open the epidermal barrier in zebrafish. In addition, zebrafish might be used as an in vivo system to investigate the capability of cCPE to enhance drug delivery across tissue barriers. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

  3. Serine Protease Inhibitor Kazal Type 1 (SPINK1) c.194+2T > C Mutation May Predict Long-term Outcome of Endoscopic Treatments in Idiopathic Chronic Pancreatitis.

    PubMed

    Sun, Chang; Liu, Mu-Yun; Liu, Xiao-Gang; Hu, Liang-Hao; Xia, Tian; Liao, Zhuan; Li, Zhao-Shen

    2015-11-01

    Endoscopic interventional is a commonly used treatment method for idiopathic chronic pancreatitis. Serine protease inhibitor Kazal type 1 (SPINK1) 194+2T>C mutation is most frequently observed in Chinese pancreatitis patients and influences the clinical course of idiopathic chronic pancreatitis patients. We conducted this study to determine the impacts of this mutation on the outcome of endoscopic treatments.In this study, we enrolled 423 patients. Among them, 101 idiopathic chronic pancreatitis patients without other relevant mutations had a successful endoscopic procedure and completed follow-up. Clinical characteristics including Izbicki pain score, exocrine and endocrine function, were evaluated. Genetic sequencing was conducted to detect SPINK1 194+2T>C mutations.The c.194+2T>C mutation was found in 58 (57.43%) patients. Factors relevant to pain relief are c.194+2T>C mutation (P = 0.011), severe pain before treatment (P = 0.005), and necessary subsequent endoscopic treatments (P < 0.001). More patients with the intronic mutation had deteriorated endocrine function (P = 0.001) relative to those patients without the mutation.Patients carrying the c.194+2T>C mutation were less likely to achieve pain relief through endoscopic treatments. They also have a higher risk of endocrine function deterioration. SPINK1 c.194+2T>C mutation may be applied as a pretreatment predictor in idiopathic chronic pancreatitis patients.

  4. 33 CFR 110.194a - Mobile Bay, Ala., and Mississippi Sound, Miss.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Sound, Miss. 110.194a Section 110.194a Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Mississippi Sound, Miss. (a) The anchorage grounds. (1) The waters of lower Mobile Bay, near Cedar Point... south by latitude 30°20′00″, and on the west by longitude 88°06′00″. (2) The waters of Mississippi Sound...

  5. 33 CFR 110.194a - Mobile Bay, Ala., and Mississippi Sound, Miss.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Sound, Miss. 110.194a Section 110.194a Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Mississippi Sound, Miss. (a) The anchorage grounds. (1) The waters of lower Mobile Bay, near Cedar Point... south by latitude 30°20′00″, and on the west by longitude 88°06′00″. (2) The waters of Mississippi Sound...

  6. 33 CFR 110.194a - Mobile Bay, Ala., and Mississippi Sound, Miss.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Sound, Miss. 110.194a Section 110.194a Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Mississippi Sound, Miss. (a) The anchorage grounds. (1) The waters of lower Mobile Bay, near Cedar Point... south by latitude 30°20′00″, and on the west by longitude 88°06′00″. (2) The waters of Mississippi Sound...

  7. 33 CFR 110.194a - Mobile Bay, Ala., and Mississippi Sound, Miss.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Sound, Miss. 110.194a Section 110.194a Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Mississippi Sound, Miss. (a) The anchorage grounds. (1) The waters of lower Mobile Bay, near Cedar Point... south by latitude 30°20′00″, and on the west by longitude 88°06′00″. (2) The waters of Mississippi Sound...

  8. 33 CFR 110.194a - Mobile Bay, Ala., and Mississippi Sound, Miss.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Sound, Miss. 110.194a Section 110.194a Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF... Mississippi Sound, Miss. (a) The anchorage grounds. (1) The waters of lower Mobile Bay, near Cedar Point... south by latitude 30°20′00″, and on the west by longitude 88°06′00″. (2) The waters of Mississippi Sound...

  9. Thermal, electronic and ductile properties of lead-chalcogenides under pressure.

    PubMed

    Gupta, Dinesh C; Bhat, Idris Hamid

    2013-09-01

    Fully relativistic pseudo-potential ab-initio calculations have been performed to investigate the high pressure phase transition, elastic and electronic properties of lead-chalcogenides including the less known lead polonium. The calculated ground state parameters, for the rock-salt structure show good agreement with the experimental data. PbS, PbSe, PbTe and PbPo undergo a first-order phase transition from rock-salt to CsCl structure at 19.4, 15.5, 11.5 and 7.3 GPa, respectively. The elastic properties have also been calculated. The calculations successfully predicted the location of the band gap at L-point of Brillouin zone and the band gap for each material at ambient pressure. It is observed that unlike other lead-chalcogenides, PbPo is semi-metal at ambient pressure. The pressure variation of the energy gap indicates that these materials metalize under pressure. The electronic structures of these materials have been computed in parent as well as in high pressure B2 phase.

  10. 33 CFR 110.194b - Mississippi Sound and Gulf of Mexico, near Petit Bois Island, Miss.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Mexico, near Petit Bois Island, Miss. 110.194b Section 110.194b Navigation and Navigable Waters COAST... Mississippi Sound and Gulf of Mexico, near Petit Bois Island, Miss. (a) The anchorage grounds—(1) Explosives... center located at latitude 30°11′12″, longitude 88°30′07″, in the waters of Gulf of Mexico south of the...

  11. Removal of Acid Black 194 dye from water by electrocoagulation with aluminum anode.

    PubMed

    Vidal, Jorge; Villegas, Loreto; Peralta-Hernández, Juan M; Salazar González, Ricardo

    2016-01-01

    Application of an electrocoagulation process (EC) for the elimination of AB194 textile dye from synthetic and textile wastewater (effluent) contaminated with AB194 dye, was carried out using aluminum anodes at two different initial pH values. Tafel studies in the presence and absence of the dye were performed. The aluminum species formed during the electrolysis were quantified by atomic absorption, and the flocs formed in the process were analyzed by HPLC-MS. Complete removal of AB194 from 1.0 L of solution was achieved applying low densities current at initial pH values of 4.0 and 8.0. The removal of AB194 by EC was possible with a short electrolysis time, removing practically 100% of the total organic carbon content and chemical oxygen demand. The final result was completely discolored water lacking dye and organic matter. An effluent contaminated with 126 mg L(-1) AB194 dye from a Chilean textile industry was also treated by EC under optimized experimental conditions, yielding discolored water and considerably decreasing the presence of organic compounds (dye + dyeing additives), with very low concentrations of dissolved Al(3+). Analysis of flocs showed the presence of the original dye without changes in its chemical structure.

  12. Role of disulphide bonds in a thermophilic serine protease aqualysin I from Thermus aquaticus YT-1.

    PubMed

    Sakaguchi, Masayoshi; Takezawa, Makoto; Nakazawa, Rie; Nozawa, Kazutaka; Kusakawa, Taro; Nagasawa, Takeshi; Sugahara, Yasusato; Kawakita, Masao

    2008-05-01

    A thermophilic serine protease, Aqualysin I, from Thermus aquaticus YT-1 has two disulphide bonds, which are also found in a psychrophilic serine protease from Vibrio sp. PA-44 and a proteinase K-like enzyme from Serratia sp. at corresponding positions. To understand the significance of these disulphide bonds in aqualysin I, we prepared mutants C99S, C194S and C99S/C194S (WSS), in which Cys69-Cys99, Cys163-Cys194 and both of these disulphide bonds, respectively, were disrupted by replacing Cys residues with Ser residues. All mutants were expressed stably in Escherichia coli. The C99S mutant was 68% as active as the wild-type enzyme at 40 degrees C in terms of k(cat) value, while C194S and WSS were only 6 and 3%, respectively, as active, indicating that disulphide bond Cys163-Cys194 is critically important for maintaining proper catalytic site conformation. Mutants C194S and WSS were less thermostable than wild-type enzyme, with a half-life at 90 degrees C of 10 min as compared to 45 min of the latter and with transition temperatures on differential scanning calorimetry of 86.7 degrees C and 86.9 degrees C, respectively. Mutant C99S was almost as stable as the wild-type aqualysin I. These results indicate that the disulphide bond Cys163-Cys194 is more important for catalytic activity and conformational stability of aqualysin I than Cys67-Cys99.

  13. An Anti-HIV-1 V3 Loop Antibody Fully Protects Cross-Clade and Elicits T-Cell Immunity in Macaques Mucosally Challenged with an R5 Clade C SHIV

    PubMed Central

    Lakhashe, Samir K.; Humbert, Michael; Sholukh, Anton; Hemashettar, Girish; Wong, Yin Ling; Yoon, John K.; Wang, Wendy; Novembre, Francis J.; Villinger, Francois; Ibegbu, Chris; Patel, Kalpana; Corti, Davide; Agatic, Gloria; Vanzetta, Fabrizia; Bianchi, Siro; Heeney, Jonathan L.; Sallusto, Federica; Lanzavecchia, Antonio; Ruprecht, Ruth M.

    2011-01-01

    Neutralizing antibodies have been shown to protect macaques against SHIV challenge. However, genetically diverse HIV-1 clades have evolved, and a key question left unanswered is whether neutralizing antibodies can confer cross-clade protection in vivo. The novel human monoclonal antibody HGN194 was isolated from an individual infected with an HIV-1 clade AG recombinant circulating recombinant form (CRF). HGN194 targets an epitope in the third hypervariable loop (V3) of HIV-1 gp120 and neutralizes a range of relatively neutralization-sensitive and resistant viruses. We evaluated the potential of HGN194 to protect infant rhesus monkeys against a SHIV encoding a primary CCR5-tropic HIV-1 clade C envelope. After high-dose mucosal challenge, all untreated controls became highly viremic while all HGN194-treated animals (50 mg/kg) were completely protected. When HGN194 was given at 1 mg/kg, one out of two monkeys remained aviremic, whereas the other had delayed, lower peak viremia. Interestingly, all protected monkeys given high-dose HGN194 developed Gag-specific proliferative responses of both CD4+ and CD8+ T cells. To test whether generation of the latter involved cryptic infection, we ablated CD8+ cells after HGN194 clearance. No viremia was detected in any protected monkeys, thus ruling out virus reservoirs. Thus, induction of CD8 T-cell immunity may have resulted from transient “Hit and Run” infection or cross priming via Ag-Ab-mediated cross-presentation. Together, our data identified the HGN194 epitope as protective and provide proof-of-concept that this anti-V3 loop mAb can prevent infection with sterilizing immunity after challenge with virus of a different clade, implying that V3 is a potential vaccine target. PMID:21483815

  14. A hydrogen-bonding network modulating enzyme function: asparagine-194 and tyrosine-225 of Escherichia coli aspartate aminotransferase.

    PubMed

    Yano, T; Mizuno, T; Kagamiyama, H

    1993-02-23

    The electron distribution within the coenzyme or coenzyme-substrate conjugate needs to be properly regulated during the catalytic process of aspartate aminotransferase (AspAT). Asn194 and Tyr225 may function in regulating the electron distribution through hydrogen-bonding to O(3') of the coenzyme, pyridoxal 5'-phosphate (PLP) or pyridoxamine 5'-phosphate (PMP). The roles of Tyr225 have already been explored by site-directed mutagenesis (Inoue et al., 1991; Goldberg et al., 1991). In the present studies, the mutant enzymes Asn194-->Ala and Asn194-->Ala + Tyr225-->Phe were analyzed kinetically and spectroscopically and were compared with the wild-type and Tyr225-->Phe enzymes. The kinetic studies showed that Asn194 is not essential for AspAT catalysis, although the Kd values for the substrates were increased by 10- to 50-fold upon the replacement of Asn194. The measurements of the absorption and fluorescence excitation spectra revealed that the ratio of an enolimine to a ketoenamine form was considerably increased as a tautomeric form of the protonated PLP in the active site of the double mutant enzyme. The pH-pKd relationship for the binding of maleate to AspAT could be explained by a simple thermodynamic cycle where only one ionizing group (the imine nitrogen of the internal aldimine bond) affects the binding of maleate. The analyses of the pH-pKd curves for the wild-type and mutant enzymes showed that (i) the hydrogen bond between O(3') of PLP and Asn194 is weakened by the binding of maleate to AspAT, while the hydrogen bond between O(3') and Tyr225 is not changed, and that (ii) the replacement of Asn194 causes some effect hampering the binding of maleate.(ABSTRACT TRUNCATED AT 250 WORDS)

  15. Crystal structures of spleen tyrosine kinase in complex with novel inhibitors: structural insights for design of anticancer drugs.

    PubMed

    Lee, Sang Jae; Choi, Jang-Sik; Han, Byeong-Gu; Kim, Hyoun Sook; Song, Ho-Juhn; Lee, Jaekyoo; Nam, Seungyoon; Goh, Sung-Ho; Kim, Jung-Ho; Koh, Jong Sung; Lee, Byung Il

    2016-10-01

    Spleen tyrosine kinase (SYK) is a cytosolic nonreceptor protein tyrosine kinase that mediates key signal transduction pathways following the activation of immune cell receptors. SYK regulates cellular events induced by the B-cell receptor and Fc receptors with high intrinsic activity. Furthermore, SYK has been regarded as an attractive target for the treatment of autoimmune diseases and cancers. Here, we report the crystal structures of SYK in complex with seven newly developed inhibitors (G206, G207, O178, O194, O259, O272, and O282) to provide structural insights into which substituents of the inhibitors and binding regions of SYK are essential for lead compound optimization. Our kinase inhibitors exhibited high inhibitory activities against SYK, with half-maximal inhibitory concentrations (IC 50 ) of approximately 0.7-33 nm, but they showed dissimilar inhibitory activities against KDR, RET, JAK2, JAK3, and FLT3. Among the seven SYK inhibitors, O272 and O282 exhibited highly specific inhibitions against SYK, whereas O194 exhibited strong inhibition of both SYK and FLT3. Three inhibitors (G206, G207, and O178) more efficiently inhibited FLT3 while still substantially inhibiting SYK activity. The binding mode analysis suggested that a highly selective SYK inhibitor can be developed by optimizing the functional groups that facilitate direct interactions with Asn499. The atomic coordinates and structure factors for human SYK are in the Protein Data Bank under accession codes 4XG2 (inhibitor-free form), 4XG3 (G206), 4XG4 (G207), 5GHV (O178), 4XG6 (O194), 4XG7 (O259), 4XG8 (O272), and 4XG9 (O282). © 2016 Federation of European Biochemical Societies.

  16. Linear clusters of galaxies - A194

    NASA Technical Reports Server (NTRS)

    Chapman, G. N. F.; Geller, M. J.; Huchra, J. P.

    1988-01-01

    New measurements for 160 redshifts and previous measurements for 108 other redshifts are presented for galaxies within 5 deg of A194. The galaxy distribution in A194 is shown to be inconsistent with a spherically symmetric King model. A mass-to-light ratio is derived using the virial theorem which is lower than the mean for the groups in the CfA redshift survey (Huchra and Geller, 1982; Geller, 1984). A nonparametric test for galaxy-cluster alignment and a Chi-squared test are used to search for alignment of galaxy major axes with the axis of A194. Evidence for neither luminosity segregation nor significant differences in the velocity or surface distributions of galaxies as a function of morphological type is found.

  17. An analysis of trends in globalisation of origin of research published in major general medical journals.

    PubMed

    Falagas, M E; Alexiou, V G

    2008-01-01

    There is an ongoing discussion in the scientific community that even the leading scientific journals publish mainly research that is produced in the countries where these journals are based. We analysed data regarding the origin of publications in 11 leading general medical journals during the last 35 years: The Lancet, British Medical Journal, Journal of the American Medical Association, New England Journal of Medicine, Annals of Internal Medicine, Archives of Internal Medicine, American Journal of Medicine, Mayo Clinic Proceedings, Canadian Medical Association Journal, Medical Journal of Australia and Journal of Internal Medicine (previously called Acta Medica Scandinavica). Among the examined journals, The Lancet has been the most diverse regarding the origin of publications; in the period 1971-1975, 62.6% of its publications originated from the UK while the relevant figure dropped to 43.2% in the period 2001-2005 (19.4% decrease). During the period 2000-2005, the proportion of publications that originated from the country in which each one of the rest of the examined journals has been based ranged from 71.7% to 95.1%. This figure decreased by a proportion ranging from 10.9% to 19.4% for some major US- and UK-based medical journals during the 35-year study period. Our own interpretation of the findings of this study is that scientific journals will better serve the global scientific community as well as the public by adopting policies that increase the mixture of the origin of research that they publish, including work from scientists in developing countries, especially during the era we live.

  18. A Recessive Mutation Resulting in a Disabling Amino Acid Substitution (T194R) in the LHX3 Homeodomain Causes Combined Pituitary Hormone Deficiency

    PubMed Central

    Bechtold-Dalla Pozza, Susanne; Hiedl, Stefan; Roeb, Julia; Lohse, Peter; Malik, Raleigh E.; Park, Soyoung; Durán-Prado, Mario; Rhodes, Simon J.

    2012-01-01

    Background/Aims Recessive mutations in the LHX3 ho-meodomain transcription factor gene are associated with developmental disorders affecting the pituitary and nervous system. We describe pediatric patients with combined pituitary hormone deficiency (CPHD) who harbor a novel mutation in LHX3. Methods Two female siblings from related parents were examined. Both patients had neonatal complications. The index patient had CPHD featuring deficiencies of GH, LH, FSH, PRL, and TSH, with later onset of ACTH deficiency. She also had a hypoplastic anterior pituitary, respiratory distress, hearing impairment, and limited neck rotation. The LHX3 gene was sequenced and the biochemical properties of the predicted altered proteins were characterized. Results A novel homozygous mutation predicted to change amino acid 194 from threonine to arginine (T194R) was detected in both patients. This amino acid is conserved in the DNA-binding homeodomain. Computer modeling predicted that the T194R change would alter the homeodomain structure. The T194R protein did not bind tested LHX3 DNA recognition sites and did not activate the α-glycoprotein and PRL target genes. Conclusion The T194R mutation affects a critical residue in the LHX3 protein. This study extends our understanding of the phenotypic features, molecular mechanism, and developmental course associated with mutations in the LHX3 gene. PMID:22286346

  19. 46 CFR 194.15-3 - Responsibility.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194... scientific party embarked may supervise the safety and operation of the chemical laboratory. (b) The...

  20. 46 CFR 194.15-3 - Responsibility.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194... scientific party embarked may supervise the safety and operation of the chemical laboratory. (b) The...

  1. 49 CFR 194.113 - Information summary.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Response Plans § 194.113 Information summary. (a) The information summary for the core plan, required by... include: (1) The information summary for the core plan; (2) The names or titles and 24-hour telephone...

  2. Association of Arg194Trp, Arg280His and Arg399Gln Polymorphisms in X-ray Repair Cross-Complementing Group 1 Gene and Risk of Differentiated Thyroid Carcinoma in Iran

    PubMed Central

    Fard-Esfahani, Pezhman; Fard-Esfahani, Armaghan; Fayaz, Shima; Ghanbarzadeh, Bahareh; Saidi, Parinaz; Mohabati, Reyhaneh; Bidoki, Seyed Kazem; Majdi, Mina

    2011-01-01

    Background: X-ray repair cross-complementing group 1 (XRCC1) gene is a DNA repair gene and its non-synonymous single nucleotide polymorphisms (SNP) may influence DNA repair capacity which has been considered as a modifying risk factor for cancer development. Methods: A case-control study was conducted to investigate impact of three frequently studied polymorphisms (Arg194Trp, Arg280His and Arg399Gln) on developing differentiated thyroid carcinoma (DTC). Results: Increased risks for DTC were shown in homozygous (odds ratio [OR]: 3.66, 95% confidence interval [CI]: 0.38-35.60) and in dominant trait (OR: 1.22, 95% CI: 1.64-2.32) of Arg194Trp genotype. Also, for Arg280His genotype, an increased risk for DTC was shown in dominant trait (OR: 1.42, 95% confidence interval [CI]: 0.76-2.68), while a mildly reduction of risk for DTC (OR: 0.77, 95% [CI]: 0.50-1.17) was estimated in dominant Gln genotype of Arg399Gln. Considering combinatory effects of Arg194Trp and Arg280His genotypes on DTC, the calculated OR and 95% CI for being heterozygous for one of Arg194Trp or Arg280His genotypes were 1.57 and 0.90-2.74, respectively. Conclusion: Genotyping of codons 194, 280 and 399 in XRCC1 gene may use in risk assessment of DTC. PMID:21987112

  3. The XRCC1 Arg194Trp polymorphism is significantly associated with lung adenocarcinoma: a case-control study in an Eastern European Caucasian group

    PubMed Central

    Cătană, Andreea; Pop, Monica; Hincu, Bianca Domokos; Pop, Ioan V; Petrişor, Felicia M; Porojan, Mihai D; Popp, Radu A

    2015-01-01

    DNA repair plays an important role in maintaining the integrity of the genome by repairing DNA damage induced by carcinogens. Certain genetic polymorphisms that occur in DNA-repair genes may affect the ability to repair DNA defects, and may represent a risk factor in carcinogenesis. The gene XRCC1 is involved in DNA repair. The purpose of our study was to investigate the association between XRCC1 Arg194Trp and Arg399Gln polymorphisms and the risk of lung cancer in a Romanian population. We recruited 222 healthy controls and 102 patients with lung cancer. Genotypes were determined by multiplex polymerase chain-reaction restriction fragment-length polymorphism. Statistical analysis (odds ratio, recessive model) revealed an increased risk for lung cancer for the homozygous 194Trp genotype (χ2=0.186, odds ratio 10.667, 95% confidence interval 1.309–86.933; P=0.007). Also, we found an association between the 194Trp allele and women with lung adenocarcinoma. In conclusion, the results of the study place the XRCC1 Arg194Trp polymorphism among independent risk factors for developing lung cancer. PMID:26664136

  4. The XRCC1 Arg194Trp polymorphism is significantly associated with lung adenocarcinoma: a case-control study in an Eastern European Caucasian group.

    PubMed

    Cătană, Andreea; Pop, Monica; Hincu, Bianca Domokos; Pop, Ioan V; Petrişor, Felicia M; Porojan, Mihai D; Popp, Radu A

    2015-01-01

    DNA repair plays an important role in maintaining the integrity of the genome by repairing DNA damage induced by carcinogens. Certain genetic polymorphisms that occur in DNA-repair genes may affect the ability to repair DNA defects, and may represent a risk factor in carcinogenesis. The gene XRCC1 is involved in DNA repair. The purpose of our study was to investigate the association between XRCC1 Arg194Trp and Arg399Gln polymorphisms and the risk of lung cancer in a Romanian population. We recruited 222 healthy controls and 102 patients with lung cancer. Genotypes were determined by multiplex polymerase chain-reaction restriction fragment-length polymorphism. Statistical analysis (odds ratio, recessive model) revealed an increased risk for lung cancer for the homozygous 194Trp genotype (χ (2)=0.186, odds ratio 10.667, 95% confidence interval 1.309-86.933; P=0.007). Also, we found an association between the 194Trp allele and women with lung adenocarcinoma. In conclusion, the results of the study place the XRCC1 Arg194Trp polymorphism among independent risk factors for developing lung cancer.

  5. 28 CFR 19.4 - Cost and percentage estimates.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... RECOVERY OF MISSING CHILDREN § 19.4 Cost and percentage estimates. It is estimated that this program will... administrative costs. It is DOJ's objective that 50 percent of DOJ penalty mail contain missing children...

  6. Islet-reactive CD8+ T cell frequencies in the pancreas, but not in blood, distinguish type 1 diabetic patients from healthy donors.

    PubMed

    Culina, Slobodan; Lalanne, Ana Ines; Afonso, Georgia; Cerosaletti, Karen; Pinto, Sheena; Sebastiani, Guido; Kuranda, Klaudia; Nigi, Laura; Eugster, Anne; Østerbye, Thomas; Maugein, Alicia; McLaren, James E; Ladell, Kristin; Larger, Etienne; Beressi, Jean-Paul; Lissina, Anna; Appay, Victor; Davidson, Howard W; Buus, Søren; Price, David A; Kuhn, Matthias; Bonifacio, Ezio; Battaglia, Manuela; Caillat-Zucman, Sophie; Dotta, Francesco; Scharfmann, Raphael; Kyewski, Bruno; Mallone, Roberto; Carel, Jean-Claude; Tubiana-Rufi, Nadia; Martinerie, Laetitia; Poidvin, Amélie; JacqzAigrain, Evelyne; Corvez, Laurence; Berruer, Véronique; Gautier, Jean-François; Baz, Baz; Haddadi, Nassima; Andreelli, Fabrizio; Amouyal, Chloé; Jaqueminet, Sophie; Bourron, Olivier; Dasque, Eric; Hartemann, Agnès; Lemoine-Yazigi, Amal; Dubois-Laforgue, Danièle; Travert, Florence; Feron, Marilyne; Rolland, Patrice; Vignali, Valérie; Marre, Michel; Chanson, Philippe; Briet, Claire; Guillausseau, Pierre-Jean; Ait-Bachir, Leila; Collet, Carole; Beziaud, Frédéric; Desforges-Bullet, Virginie; Petit-Aubert, Gwenaelle; Christin-Maitre, Sophie; Fève, Bruno; Vatier, Camille; Bourcigaux, Nathalie; Lautridou, Céline; Lahlou, Najiba; Bakouboula, Prissile; Elie, Caroline; Morel, Hélène; Treluyer, Jean-Marc; Gagnerault, Marie-Claude; Maillard, Claire; Jones, Anna

    2018-02-02

    The human leukocyte antigen-A2 (HLA-A2)-restricted zinc transporter 8 186-194 (ZnT8 186-194 ) and other islet epitopes elicit interferon-γ secretion by CD8 + T cells preferentially in type 1 diabetes (T1D) patients compared with controls. We show that clonal ZnT8 186-194 -reactive CD8 + T cells express private T cell receptors and display equivalent functional properties in T1D and healthy individuals. Ex vivo analyses further revealed that CD8 + T cells reactive to ZnT8 186-194 and other islet epitopes circulate at similar frequencies and exhibit a predominantly naïve phenotype in age-matched T1D and healthy donors. Higher frequencies of ZnT8 186-194 -reactive CD8 + T cells with a more antigen-experienced phenotype were detected in children versus adults, irrespective of disease status. Moreover, some ZnT8 186-194 -reactive CD8 + T cell clonotypes were found to cross-recognize a Bacteroides stercoris mimotope. Whereas ZnT8 was poorly expressed in thymic medullary epithelial cells, variable thymic expression levels of islet antigens did not modulate the peripheral frequency of their cognate CD8 + T cells. In contrast, ZnT8 186-194 -reactive cells were enriched in the pancreata of T1D patients versus nondiabetic and type 2 diabetic individuals. Thus, islet-reactive CD8 + T cells circulate in most individuals but home to the pancreas preferentially in T1D patients. We conclude that the activation of this common islet-reactive T cell repertoire and progression to T1D likely require defective peripheral immunoregulation and/or a proinflammatory islet microenvironment. Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

  7. Novel Synthetic, Host-defense Peptide Protects Against Organ Injury/Dysfunction in a Rat Model of Severe Hemorrhagic Shock.

    PubMed

    Yamada, Noriaki; Martin, Lukas B; Zechendorf, Elisabeth; Purvis, Gareth S D; Chiazza, Fausto; Varrone, Barbara; Collino, Massimo; Shepherd, Joanna; Heinbockel, Lena; Gutsmann, Thomas; Correa, Wilmar; Brandenburg, Klaus; Marx, Gernot; Schuerholz, Tobias; Brohi, Karim; Thiemermann, Christoph

    2017-03-10

    To evaluate (1) levels of the host-defense/antimicrobial peptide LL-37 in patients with trauma and hemorrhagic shock (HS) and (2) the effects of a synthetic host-defense peptide; Pep19-4LF on multiple organ failure (MOF) associated with HS. HS is a common cause of death in severely injured patients. There is no specific therapy that reduces HS-associated MOF. (1) LL-37 was measured in 47 trauma/HS patients admitted to an urban major trauma center. (2) Male Wistar rats were submitted to HS (90 min, target mean arterial pressure: 27-32 mm Hg) or sham operation. Rats were treated with Pep19-4LF [66 (n = 8) or 333 μg/kg · h (n = 8)] or vehicle (n = 12) for 4 hours following resuscitation. Plasma LL-37 was 12-fold higher in patients with trauma/HS compared to healthy volunteers. HS rats treated with Pep19-4LF (high dose) had a higher mean arterial pressure at the end of the 4-hour resuscitation period (79 ± 4 vs 54 ± 5 mm Hg) and less renal dysfunction, liver injury, and lung inflammation than HS rats treated with vehicle. Pep19-4LF enhanced (kidney/liver) the phosphorylation of (1) protein kinase B and (2) endothelial nitric oxide synthase. Pep19-4LF attenuated the HS-induced (1) translocation of p65 from cytosol to nucleus, (2) phosphorylation of IκB kinase on Ser, and (3) phosphorylation of IκBα on Ser resulting in inhibition of nuclear factor kappa B and formation of proinflammatory cytokines. Pep19-4LF prevented the release of tumor necrosis factor alpha caused by heparan sulfate in human mononuclear cells by binding to this damage-associated molecular pattern. Trauma-associated HS results in release of LL-37. The synthetic host-defense/antimicrobial peptide Pep19-4LF attenuates the organ injury/dysfunction associated with HS.

  8. The contribution of the SPINK1 c.194+2T>C mutation to the clinical course of idiopathic chronic pancreatitis in Chinese patients.

    PubMed

    Sun, Chang; Liao, Zhuan; Jiang, Lili; Yang, Fu; Xue, Geng; Zhou, Qi; Chen, Ruiwen; Sun, Shuhan; Li, Zhaoshen

    2013-01-01

    Recent data suggest that the serine protease inhibitor Kazal type 1 (SPINK1) gene mutation is associated with idiopathic chronic pancreatitis. However, few studies have focused on the serine protease inhibitor Kazal type 1 c.194+2T>C mutation. Therefore, our goal was to study the prevalence and impact of serine protease inhibitor Kazal type 1 mutations on the clinical profile of idiopathic chronic pancreatitis patients in China. A retrospective-cohort study of 118 Chinese patients with idiopathic chronic pancreatitis was performed, and genetic tests were carried out to detect SPINK1 mutations. Subjects without pancreatitis were used as controls. In total, 118 idiopathic chronic pancreatitis patients and 100 control subjects were evaluated. The serine protease inhibitor Kazal type 1 c.194+2T>C variant was present in 44.9% of patients with idiopathic chronic pancreatitis. The frequency of diabetes in idiopathic chronic pancreatitis patients with the serine protease inhibitor Kazal type 1 c.194+2T>C mutation (39.6%) was higher than that of patients without the mutation (9.2%). The time to occurrence of diabetes mellitus after idiopathic chronic pancreatitis symptom onset is significantly influenced by the c.194+2T>C mutation (p<0.001). In addition, the mean age of diabetes onset in patients with the serine protease inhibitor Kazal type 1 c.194+2T>C mutation (38.33 ± 9.50) was significantly younger than that of patients without this mutation (49.67 ± 6.74). The presence of the serine protease inhibitor Kazal type 1 c.194+2T>C mutation seems to be associated with idiopathic chronic pancreatitis and could predispose individuals to pancreatic diabetes onset at an earlier age. Copyright © 2012 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.

  9. Identification of miR-194-5p as a potential biomarker for postmenopausal osteoporosis

    PubMed Central

    Pan, Nanan; Sun, Ning; Wang, Qiujun; Fan, Jingxue; Zhou, Ping

    2015-01-01

    The incidence of osteoporosis is high in postmenopausal women due to altered estrogen levels and continuous calcium loss that occurs with aging. Recent studies have shown that microRNAs (miRNAs) are involved in the development of osteoporosis. These miRNAs may be used as potential biomarkers to identify women at a high risk for developing the disease. In this study, whole blood samples were collected from 48 postmenopausal Chinese women with osteopenia or osteoporosis and pooled into six groups according to individual T-scores. A miRNA microarray analysis was performed on pooled blood samples to identify potential miRNA biomarkers for postmenopausal osteoporosis. Five miRNAs (miR-130b-3p, -151a-3p, -151b, -194-5p, and -590-5p) were identified in the microarray analysis. These dysregulated miRNAs were subjected to a pathway analysis investigating whether they were involved in regulating osteoporosis-related pathways. Among them, only miR-194-5p was enriched in multiple osteoporosis-related pathways. Enhanced miR-194-5p expression in women with osteoporosis was confirmed by quantitative reverse transcription–polymerase chain reaction analysis. For external validation, a significant correlation between the expression of miR-194-5p and T-scores was found in an independent patient collection comprised of 24 postmenopausal women with normal bone mineral density, 30 postmenopausal women with osteopenia, and 32 postmenopausal women with osteoporosis (p < 0.05). Taken together, the present findings suggest that miR-194-5p may be a viable miRNA biomarker for postmenopausal osteoporosis. PMID:26038726

  10. Identification of miR-194-5p as a potential biomarker for postmenopausal osteoporosis.

    PubMed

    Meng, Jia; Zhang, Dapeng; Pan, Nanan; Sun, Ning; Wang, Qiujun; Fan, Jingxue; Zhou, Ping; Zhu, Wenliang; Jiang, Lihong

    2015-01-01

    The incidence of osteoporosis is high in postmenopausal women due to altered estrogen levels and continuous calcium loss that occurs with aging. Recent studies have shown that microRNAs (miRNAs) are involved in the development of osteoporosis. These miRNAs may be used as potential biomarkers to identify women at a high risk for developing the disease. In this study, whole blood samples were collected from 48 postmenopausal Chinese women with osteopenia or osteoporosis and pooled into six groups according to individual T-scores. A miRNA microarray analysis was performed on pooled blood samples to identify potential miRNA biomarkers for postmenopausal osteoporosis. Five miRNAs (miR-130b-3p, -151a-3p, -151b, -194-5p, and -590-5p) were identified in the microarray analysis. These dysregulated miRNAs were subjected to a pathway analysis investigating whether they were involved in regulating osteoporosis-related pathways. Among them, only miR-194-5p was enriched in multiple osteoporosis-related pathways. Enhanced miR-194-5p expression in women with osteoporosis was confirmed by quantitative reverse transcription-polymerase chain reaction analysis. For external validation, a significant correlation between the expression of miR-194-5p and T-scores was found in an independent patient collection comprised of 24 postmenopausal women with normal bone mineral density, 30 postmenopausal women with osteopenia, and 32 postmenopausal women with osteoporosis (p < 0.05). Taken together, the present findings suggest that miR-194-5p may be a viable miRNA biomarker for postmenopausal osteoporosis.

  11. [Relation of MBL ExonI 54 and NFκB1-94ins/del ATTG Polymorphism with Fever during Neutropenia in Patients with Acute Leukaemia after Chemotherapy].

    PubMed

    Xu, Wen-Ning; Jiang, Zu-Jun; Li, Yong-Hua; Xiao, Hao-Wen; Gao, Yang; Pang, Yan; Ouyang, Lin; Liu, Zeng-Hui; Zhang, Le-Qing; Wang, Yang; Xiao, Yang

    2015-10-01

    To explore the correlation between MBL ExonI 54 and NFκB1-94ins/del ATTG polymorphism and fever during neutropenia in patients with acute leukaemia (AL) (except M3) after first chemotherapy in Chinese Han population. Blood samples obtained from 76 fever patients with AL during neutropenia episodes were detected to analyse single nucleotide polymorphism (SNP) in the MBL ExonI 54 and NFκB1-94ins/del ATTG gene, and analyse the correlation between above-mentioned 2 polymorphisms and fever during neutropenia of AL patients after chemotherapy. In 76 patients, no correlation were found between MBL ExonI 54 and NFκB1-94ins/del ATTG polymorphism and fever during neutropenia in patients with acute leukaemia after chemotherapy (P > 0.05). No significant relation were found in sex, age, underlying disease, disease status or degrees of neutropenia in febrile neutropenia between MBL ExonI 54 and NFκB1-94ins/del ATTG polymorphism (P > 0.05). However, patients with MBL ExonI 54 mutation presented longer febrile duration with a median of 5 days compared to 3 days of patients with wildtype MBL ExonI 54 genotype (P < 0.05). There is no clear correlation between MBL ExonI 54 and NFκB1-94ins/del ATTG polymorphism and fever during neutropenia in patients with acute leukaemia after chemotherapy. However, the patients with MBL ExonI 54 mutation have been observed to present a longer febrile duration.

  12. 27 CFR 46.194 - Persons not liable for floor stocks tax.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... TOBACCO PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 General § 46.194 Persons not liable for floor...

  13. 27 CFR 46.194 - Persons not liable for floor stocks tax.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... TOBACCO PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 General § 46.194 Persons not liable for floor...

  14. 27 CFR 46.194 - Persons not liable for floor stocks tax.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... TOBACCO PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 General § 46.194 Persons not liable for floor...

  15. 27 CFR 46.194 - Persons not liable for floor stocks tax.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... TOBACCO PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 General § 46.194 Persons not liable for floor...

  16. 27 CFR 46.194 - Persons not liable for floor stocks tax.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... TOBACCO PRODUCTS AND CIGARETTE PAPERS AND TUBES Floor Stocks Tax on Certain Tobacco Products, Cigarette Papers, and Cigarette Tubes Held for Sale on April 1, 2009 General § 46.194 Persons not liable for floor...

  17. Serum neuron-specific enolase, biogenic amino-acids and neurobehavioral function in lead-exposed workers from lead-acid battery manufacturing process.

    PubMed

    Ravibabu, K; Barman, T; Rajmohan, H R

    2015-01-01

    The interaction between serum neuron-specific enolase (NSE), biogenic amino-acids and neurobehavioral function with blood lead levels in workers exposed to lead form lead-acid battery manufacturing process was not studied. To evaluate serum NSE and biogenic amino-acids (dopamine and serotonin) levels, and neurobehavioral performance among workers exposed to lead from lead-acid storage battery plant, and its relation with blood lead levels (BLLs). In a cross-sectional study, we performed biochemical and neurobehavioral function tests on 146 workers exposed to lead from lead-acid battery manufacturing process. BLLs were assessed by an atomic absorption spectrophotometer. Serum NSE, dopamine and serotonin were measured by ELISA. Neurobehavioral functions were assessed by CDC-recommended tests---simple reaction time (SRT), symbol digit substitution test (SDST), and serial digit learning test (SDLT). There was a significant correlation (r 0.199, p<0.05) between SDST and BLL. SDLT and SRT had also a significant positive correlation (r 0.238, p<0.01). NSE had a negative correlation (r -0.194, p<0.05) with serotonin level. Multiple linear regression analysis revealed that both SRT and SDST had positive significant associations with BLL. SRT also had a positive significant association with age. Serum NSE cannot be used as a marker for BLL. The only domain of neurobehavioral function tests that is affected by increased BLL in workers of lead-acid battery manufacturing process is that of the "attention and perception" (SDST).

  18. 14 CFR Sec. 19-4 - Service classes.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... a composite of first class, coach, and mixed passenger/cargo service. The following classifications... integral part of services performed pursuant to published flight schedules. The following classifications... Classifications Sec. 19-4 Service classes. The statistical classifications are designed to reflect the operating...

  19. 14 CFR Sec. 19-4 - Service classes.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... a composite of first class, coach, and mixed passenger/cargo service. The following classifications... integral part of services performed pursuant to published flight schedules. The following classifications... Classifications Sec. 19-4 Service classes. The statistical classifications are designed to reflect the operating...

  20. Association of XRCC1 Trp194 allele with risk of breast cancer, and Ki67 protein status in breast tumor tissues

    PubMed Central

    Jalali, Chiya; Ghaderi, Bayazid; Amini, Sabrieh; Abdi, Mohammad; Roshani, Daem

    2016-01-01

    Objectives: To evaluate the role of this polymorphism as a risk factor for breast cancer in Kurdish patients and to investigate the possible association between Arg194Trp x-ray repair cross-complementing group 1 (XRCC1) gene polymorphisms with clinical and histopathological outcomes of patients with breast cancer. Methods: A total of 100 breast cancer patients and 200 cancer-free controls in Kurdish population of Kurdistan state admitted to Tohid Hospital, Sanandaj, Kurdistan, Iran between January 2012 and May 2015 were enrolled in this cross-sectional study. Tissue expression of estrogen receptor (ER), progesteron receptor (PR), human epidermal growth factor receptor 2 (Her2/neu), and Ki67 were evaluated by immunohistochemistry (IHC). The Arg194Trp genotypes were determined by polymerase chain reaction- restriction fragment length polymorphism method. Results: Our data showed that the risk for breast cancer increased significantly among the Trp variant of XRCC1. Statistically significant association was found between codon 194 polymorphisms and tissue expression of Ki67. Conclusion: The Trp allele of codon 194 XRCC1 is a potential risk factor for breast cancer in Kurdish ethnicity. Furthermore, effect of this polymorphism on clinical and histological features of breast cancer was significant. PMID:27279507

  1. 46 CFR 194.15-5 - Ventilation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194... be equipped with acceptable flame screens. (b) Chemical laboratories shall be equipped with power...) Ventilation of air conditioning systems serving the chemical laboratory shall be designed so that air cannot...

  2. 46 CFR 194.15-5 - Ventilation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194... be equipped with acceptable flame screens. (b) Chemical laboratories shall be equipped with power...) Ventilation of air conditioning systems serving the chemical laboratory shall be designed so that air cannot...

  3. 21 CFR 155.194 - Catsup.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION CANNED VEGETABLES Requirements for Specific Standardized Canned Vegetables § 155.194 Catsup. (a... be adjusted by concentration and/or by the addition of water. The food may contain salt (sodium...

  4. 46 CFR 194.15-1 - General.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-1 General..., such as desks, file and storage cabinets, waste paper baskets, work benches, chair frames, etc. Working...

  5. 46 CFR 194.15-1 - General.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-1 General..., such as desks, file and storage cabinets, waste paper baskets, work benches, chair frames, etc. Working...

  6. 46 CFR 194.15-1 - General.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS HANDLING, USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-1 General..., such as desks, file and storage cabinets, waste paper baskets, work benches, chair frames, etc. Working...

  7. 30 CFR 250.194 - How must I protect archaeological resources?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 2 2012-07-01 2012-07-01 false How must I protect archaeological resources... Information and Reporting Requirements § 250.194 How must I protect archaeological resources? (a)-(b... Regional Director will tell you how to protect it. ...

  8. 30 CFR 250.194 - How must I protect archaeological resources?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 2 2014-07-01 2014-07-01 false How must I protect archaeological resources... Information and Reporting Requirements § 250.194 How must I protect archaeological resources? (a)-(b... Regional Director will tell you how to protect it. ...

  9. 30 CFR 250.194 - How must I protect archaeological resources?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false How must I protect archaeological resources... Information and Reporting Requirements § 250.194 How must I protect archaeological resources? (a)-(b... Regional Director will tell you how to protect it. ...

  10. The water hexamer: three-body interactions, structures, energetics, and OH-stretch spectroscopy at finite temperature.

    PubMed

    Tainter, C J; Skinner, J L

    2012-09-14

    Using a newly developed and recently parameterized classical empirical simulation model for water that involves explicit three-body interactions, we determine the eleven most stable isomers of the water hexamer. We find that the lowest energy isomer is one of the cage structures, in agreement with far-IR and microwave experiments. The energy ordering for the binding energies is cage > glove > book > bag > chair > boat > chaise, and energies relative to the cage are in good agreement with CCSD(T) calculations. The three-body contributions to the cage, book, and chair are also in reasonable agreement with CCSD(T) results. The energy of each isomer results from a delicate balance involving the number of hydrogen bonds, the strain of these hydrogen bonds, and cooperative and anti-cooperative three-body interactions, whose contribution we can understand simply from the form of the three-body interactions in the simulation model. Oxygen-oxygen distances in the cage and book isomers are in good agreement with microwave experiments. Hydrogen-bond distances depend on both donor and acceptor, which can again be understood from the three-body model. Fully anharmonic OH-stretch spectra are calculated for these low-energy structures, and compared with shifted harmonic results from ab initio and density functional theory calculations. Replica-exchange molecular dynamics simulations were performed from 40 to 194 K, which show that the cage isomer has the lowest free energy from 0 to 70 K, and the book isomer has the lowest free energy from 70 to 194 K. OH-stretch spectra were calculated between 40 and 194 K, and results at 40, 63, and 79 K were compared to recent experiments, leading to re-assignment of the peaks in the experimental spectra. We calculate local OH-stretch cumulative spectral densities for different donor-acceptor types and compare to analogous results for liquid water.

  11. [Creative mathematics with clopidogrel; exaggeration of the preventive effect by manufacturer].

    PubMed

    Algra, A; van Gijn, J; Kappelle, L J; Koudstaal, P J; Stam, J; Vermeulen, M

    1999-12-04

    A number of Dutch medical journals recently carried an advertisement stating that clopidogrel treatment reduced the number of ischaemic complications with 26%, compared with aspirin treatment. This is a miscalculation: the actual reduction is 0.51% in absolute rates, and 8.7% in relative terms. The error by Sanofi-Synthelabo arose by comparison of the event rates for clopidogrel (5.32%) as well as for aspirin (5.83%) with that in an imaginary placebo group (7.77%), yielding a reduction of ischaemic complications of 2.45% and 1.94% respectively; erroneous comparison of these two numbers leads to a difference of 26%.

  12. Genomic location of the bovine growth hormone secretagogue receptor (GHSR) gene and investigation of genetic polymorphism.

    PubMed

    Colinet, F G; Vanderick, S; Charloteaux, B; Eggen, A; Gengler, N; Renaville, B; Brasseur, R; Portetelle, D; Renaville, Robert

    2009-01-01

    The growth hormone secretagogue receptor (GHSR) is involved in the regulation of energetic homeostasis and GH secretion. In this study, the bovine GHSR gene was mapped to BTA1 between BL26 and BMS4004. Two different bovine GHSR CDS (GHSR1a and GHSR1b) were sequenced. Six polymorphisms (five SNPs and one 3-bp indel) were also identified, three of them leading to amino acid variations L24V, D194N, and Del R242. These variations are located in the extracellular N-terminal end, the exoloop 2, and the cytoloop 3 of the receptor, respectively.

  13. Modeling and Re-Engineering of Azotobacter vinelandii Alginate Lyase to Enhance Its Catalytic Efficiency for Accelerating Biofilm Degradation.

    PubMed

    Jang, Chul Ho; Piao, Yu Lan; Huang, Xiaoqin; Yoon, Eun Jeong; Park, So Hee; Lee, Kyoung; Zhan, Chang-Guo; Cho, Hoon

    2016-01-01

    Alginate is known to prevent elimination of Pseudomonas aeruginosa biofilms. Alginate lyase (AlgL) might therefore facilitate treatment of Pseudomonas aeruginosa-infected cystic fibrosis patients. However, the catalytic activity of wild-type AlgL is not sufficiently high. Therefore, molecular modeling and site-directed mutagenesis of AlgL might assist in enzyme engineering for therapeutic development. AlgL, isolated from Azotobacter vinelandii, catalyzes depolymerization of alginate via a β-elimination reaction. AlgL was modeled based on the crystal structure template of Sphingomonas AlgL species A1-III. Based on this computational analysis, AlgL was subjected to site-directed mutagenesis to improve its catalytic activity. The kcat/Km of the K194E mutant showed a nearly 5-fold increase against the acetylated alginate substrate, as compared to the wild-type. Double and triple mutants (K194E/K245D, K245D/K319A, K194E/K245D/E312D, and K194E/K245D/K319A) were also prepared. The most potent mutant was observed to be K194E/K245D/K319A, which has a 10-fold improved kcat value (against acetylated alginate) compared to the wild-type enzyme. The antibiofilm effect of both AlgL forms was identified in combination with piperacillin/tazobactam (PT) and the disruption effect was significantly higher in mutant AlgL combined with PT than wild-type AlgL. However, for both the wild-type and K194E/K245D/K319A mutant, the use of the AlgL enzyme alone did not show significant antibiofilm effect.

  14. 40 CFR 194.24 - Waste characterization.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... other information and methods. (b) The Department shall submit in the compliance certification... proposed for disposal in the disposal system, WIPP complies with the numeric requirements of § 194.34 and... release. (2) Identify and describe the method(s) used to quantify the limits of waste components...

  15. 30 CFR 19.4 - Conditions governing investigations.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 19.4 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR TESTING.... This material should be sent prepaid to the U.S. Department of Labor, Mine Safety and Health Administration, Approval and Certification Center, 765 Technology Drive, Triadelphia, WV 26059. (b) When this...

  16. 10 CFR 19.4 - Interpretations.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... REGULATORY COMMISSION NOTICES, INSTRUCTIONS AND REPORTS TO WORKERS: INSPECTION AND INVESTIGATIONS § 19.4 Interpretations. Except as specifically authorized by the Commission in writing, no interpretation of the meaning of the regulations in this part by any officer or employee of the Commission other than a written...

  17. The correlation of Skylab L-band brightness temperatures with antecedent precipitation

    NASA Technical Reports Server (NTRS)

    Mcfarland, M. J.

    1975-01-01

    The S194 L-band radiometer flown on the Skylab mission measured terrestrial radiation at the microwave wavelength of 21.4 cm. The terrain emissivity at this wavelength is strongly dependent on the soil moisture content, which can be inferred from antecedent precipitation. For the Skylab data acquisition pass from the Oklahoma panhandle to southeastern Texas on 11 June 1973, the S194 brightness temperatures are highly correlated with antecedent precipitation from the preceding eleven day period, but very little correlation was apparent for the preceding five day period. The correlation coefficient between the averaged antecedent precipitation index values and the corresponding S194 brightness temperatures between 230 K and 270 K, the region of apparent response to soil moisture in the data, was -0.97. The equation of the linear least squares line fitted to the data was: API (cm) = 31.99 -0.114 TB (K), where API is the antecedent precipitation index and TB is the S194 brightness temperature.

  18. The process of S-nitrosation in sGC β1(1-194) revealed by infrared spectroscopy

    NASA Astrophysics Data System (ADS)

    Liu, Li; Wang, Dandan; Xu, Haoran; Mi, Mengdan; Li, Zhengqiang

    2015-06-01

    Soluble guanylate cyclase (sGC) is the most important receptor for the signaling molecule NO. NO activates sGC by binding to its heme cofactor and reacts with free thiols in the protein itself. The S-nitrosation of cysteine thiols affects the activity of soluble guanylate cyclase (sGC). In this study, infrared (IR) spectroscopy and site-directed mutagenesis were used to investigate S-H vibration and the process of S-nitrosation in the β1 subunit (amino acids 1-194) of sGC. Fourier transform IR spectroscopy revealed that wild-type and mutants (C78S and C122S) of sGC β1(1-194) exhibited S-H peaks around 2560 cm-1. The signals were attenuated in the IR spectra of S-nitrosoglutathione-treated mutants, demonstrating that S-nitrosation in sGC β1(1-194) occured at residues C78 and C122, and the process of the reaction was GSNO concentration-dependent.

  19. All-fiber laser at 1.94 µm: effect on soft tissue

    NASA Astrophysics Data System (ADS)

    Pal, Atasi; Pal, Debasis; Das Chowdhury, Sourav; Sen, Ranjan

    2017-02-01

    A focused laser beam at wavelength of strong water absorption at 1.94 μm can be a good scalpel for precision soft tissue surgery. A fiber Bragg grating-based, all-fiber, continuous-wave as well as modulated, cladding pumped, thulium-doped fiber laser at 1.94 μm has been configured to deliver up to 10 W of laser power under pumping at 793 nm having an efficiency of 32 %. The laser was exposed to freshly sacrificed chicken breast at different power level and exposure time. The formalin-fixed samples were examined by microscopy to identify the ablation region, carbonization and necrosis region for laser parameter optimization.

  20. Sequence of pNL194, a 79.3-Kilobase IncN Plasmid Carrying the blaVIM-1 Metallo-β-Lactamase Gene in Klebsiella pneumoniae▿

    PubMed Central

    Miriagou, V.; Papagiannitsis, C. C.; Kotsakis, S. D.; Loli, A.; Tzelepi, E.; Legakis, N. J.; Tzouvelekis, L. S.

    2010-01-01

    The nucleotide sequence of pNL194, a VIM-1-encoding plasmid, is described in this study. pNL194 (79,307 bp) comprised an IncN-characteristic segment (38,940 bp) and a mosaic structure (40,367 bp) including blaVIM-1, aacA7, aadA1, aadA2, dfrA1, dfrA12, aphA1, strA, strB, and sul1. Tn1000 or Tn5501 insertion within fipA probably facilitated recruitment of additional mobile elements carrying resistance genes. PMID:20660690

  1. 46 CFR 194.15-11 - Flushing systems.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ..., AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-11 Flushing systems. (a) Working spaces in which chemical stores are used shall be equipped with a fresh water supply shower. (b) There shall be a provision for flushing away chemical spills. ...

  2. 46 CFR 194.15-11 - Flushing systems.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-11 Flushing systems. (a) Working spaces in which chemical stores are used shall be equipped with a fresh water supply shower. (b) There shall be a provision for flushing away chemical spills. ...

  3. 46 CFR 194.15-11 - Flushing systems.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemistry Laboratory and Scientific Laboratory § 194.15-11 Flushing systems. (a) Working spaces in which chemical stores are used shall be equipped with a fresh water supply shower. (b) There shall be a provision for flushing away chemical spills. ...

  4. 7 CFR 4280.194 - Actions prior to grant closing.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE LOANS AND GRANTS Rural Energy for America Program General Energy Audit and Renewable Energy Development Assistance Grants § 4280.194 Actions prior to grant closing. Applicants expecting funds from other sources for use in completing projects being...

  5. 7 CFR 4280.194 - Actions prior to grant closing.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE LOANS AND GRANTS Rural Energy for America Program General Energy Audit and Renewable Energy Development Assistance Grants § 4280.194 Actions prior to grant closing. Applicants expecting funds from other sources for use in completing projects being...

  6. 7 CFR 4280.194 - Actions prior to grant closing.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE LOANS AND GRANTS Rural Energy for America Program General Energy Audit and Renewable Energy Development Assistance Grants § 4280.194 Actions prior to grant closing. Applicants expecting funds from other sources for use in completing projects being...

  7. 40 CFR 194.51 - Consideration of protected individual.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Consideration of protected individual... Individual and Ground-Water Protection Requirements § 194.51 Consideration of protected individual. Compliance assessments that analyze compliance with § 191.15 of this chapter shall assume that an individual...

  8. 40 CFR 194.52 - Consideration of exposure pathways.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Consideration of exposure pathways... Individual and Ground-Water Protection Requirements § 194.52 Consideration of exposure pathways. In compliance assessments that analyze compliance with § 191.15 of this chapter, all potential exposure pathways...

  9. 40 CFR 194.52 - Consideration of exposure pathways.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Consideration of exposure pathways... Individual and Ground-Water Protection Requirements § 194.52 Consideration of exposure pathways. In compliance assessments that analyze compliance with § 191.15 of this chapter, all potential exposure pathways...

  10. 40 CFR 194.52 - Consideration of exposure pathways.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Consideration of exposure pathways... Individual and Ground-Water Protection Requirements § 194.52 Consideration of exposure pathways. In compliance assessments that analyze compliance with § 191.15 of this chapter, all potential exposure pathways...

  11. 40 CFR 194.52 - Consideration of exposure pathways.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Consideration of exposure pathways... Individual and Ground-Water Protection Requirements § 194.52 Consideration of exposure pathways. In compliance assessments that analyze compliance with § 191.15 of this chapter, all potential exposure pathways...

  12. 40 CFR 194.44 - Engineered barriers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... prevent or substantially delay the movement of water or waste toward the accessible environment; (ii) The... reduced total system costs; (viii) The impact, if any, on other waste disposal programs from the... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Engineered barriers. 194.44 Section...

  13. 40 CFR 194.44 - Engineered barriers.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... prevent or substantially delay the movement of water or waste toward the accessible environment; (ii) The... reduced total system costs; (viii) The impact, if any, on other waste disposal programs from the... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Engineered barriers. 194.44 Section...

  14. 41 CFR 102-194.30 - What role does my agency play in the Standard and Optional Forms Management Program?

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... with both an electronic (unless exempted by § 102-194.40) and paper version of the official image of... forms through GSA for the approval of the Interagency Committee on Medical Records (ICMR). (j) Promote...

  15. 41 CFR 102-194.30 - What role does my agency play in the Standard and Optional Forms Management Program?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... with both an electronic (unless exempted by § 102-194.40) and paper version of the official image of... forms through GSA for the approval of the Interagency Committee on Medical Records (ICMR). (j) Promote...

  16. 41 CFR 102-194.30 - What role does my agency play in the Standard and Optional Forms Management Program?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... with both an electronic (unless exempted by § 102-194.40) and paper version of the official image of... forms through GSA for the approval of the Interagency Committee on Medical Records (ICMR). (j) Promote...

  17. 41 CFR 102-194.30 - What role does my agency play in the Standard and Optional Forms Management Program?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... with both an electronic (unless exempted by § 102-194.40) and paper version of the official image of... forms through GSA for the approval of the Interagency Committee on Medical Records (ICMR). (j) Promote...

  18. 41 CFR 102-194.30 - What role does my agency play in the Standard and Optional Forms Management Program?

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... with both an electronic (unless exempted by § 102-194.40) and paper version of the official image of... forms through GSA for the approval of the Interagency Committee on Medical Records (ICMR). (j) Promote...

  19. 40 CFR 194.24 - Waste characterization.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... system. (e) Waste may be emplaced in the disposal system only if the emplaced components of such waste... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Waste characterization. 194.24 Section... PROGRAMS CRITERIA FOR THE CERTIFICATION AND RE-CERTIFICATION OF THE WASTE ISOLATION PILOT PLANT'S...

  20. 40 CFR 194.24 - Waste characterization.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... system. (e) Waste may be emplaced in the disposal system only if the emplaced components of such waste... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Waste characterization. 194.24 Section... PROGRAMS CRITERIA FOR THE CERTIFICATION AND RE-CERTIFICATION OF THE WASTE ISOLATION PILOT PLANT'S...

  1. 40 CFR 194.24 - Waste characterization.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... system. (e) Waste may be emplaced in the disposal system only if the emplaced components of such waste... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Waste characterization. 194.24 Section... PROGRAMS CRITERIA FOR THE CERTIFICATION AND RE-CERTIFICATION OF THE WASTE ISOLATION PILOT PLANT'S...

  2. 40 CFR 194.24 - Waste characterization.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... system. (e) Waste may be emplaced in the disposal system only if the emplaced components of such waste... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Waste characterization. 194.24 Section... PROGRAMS CRITERIA FOR THE CERTIFICATION AND RE-CERTIFICATION OF THE WASTE ISOLATION PILOT PLANT'S...

  3. Measurement of activation cross sections of alpha particle induced reactions on iridium up to an energy of 50 MeV.

    PubMed

    Takács, S; Ditrói, F; Szűcs, Z; Aikawa, M; Haba, H; Komori, Y; Saito, M

    2018-06-01

    Cross sections of alpha particle induced nuclear reactions on iridium were investigated using a 51.2-MeV alpha particle beam. The standard stacked-foil target technique and the activation method were applied. The activity of the reaction products was assessed without chemical separation using high resolution gamma-ray spectrometry. Excitation functions for production of gold, platinum and iridium isotopes ( 196m2 Au, 196m,g Au, 195m,g Au, 194 Au, 193 m,g Au, 192 Au, 191m,g Au, 191 Pt, 195m Pt, 194g Ir, 194m Ir, 192g Ir, 190g Ir and 189 Ir) were determined and compared with available earlier measured experimental data and results of theoretical calculations using TALYS code system. Cross section data were reported for the first time for the nat Ir(α,x) 196m2 Au, nat Ir(α,x) 196m,g Au, nat Ir(α,x) 191 Pt, nat Ir(α,x) 195m Pt, nat Ir(α,x) 194g Ir, nat Ir(α,x) 194m Ir, nat Ir(α,x) 190g Ir and nat Ir(α,x) 189 Ir processes. A possible production route for 195m Pt, the potentially important radionuclide in nuclear medicine, is discussed. Copyright © 2018 Elsevier Ltd. All rights reserved.

  4. Soldier-relevant body borne load impacts minimum foot clearance during obstacle negotiation.

    PubMed

    Brown, T N; Loverro, K L; Schiffman, J M

    2016-07-01

    Soldiers often trip and fall on duty, resulting in injury. This study examined ten male soldiers' ability to negotiate an obstacle. Participants had lead and trail foot minimum foot clearance (MFC) parameters quantified while crossing a low (305 mm) and high (457 mm) obstacle with (19.4 kg) and without (6 kg) body borne load. To minimize tripping risk, participants increased lead foot MFC (p = 0.028) and reduced lead (p = 0.044) and trail (p = 0.035) foot variability when negotiating an obstacle with body borne load. While obstacle height had no effect on MFC (p = 0.273 and p = 0.126), placing the trail foot closer to the high obstacle when crossing with body borne load, resulted in greater lead (R = 0.640, b = 0.241, p = 0.046) and trail (R = 0.636, b = 0.287, p = 0.048) MFC. Soldiers, when carrying typical military loads, may be able to minimize their risk of tripping over an obstacle by creating a safety margin via greater foot clearance with reduced variability. Published by Elsevier Ltd.

  5. 34 CFR 668.194 - Economically disadvantaged appeals.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Rates § 668.194 Economically disadvantaged appeals. (a) Eligibility. As described in this section, you... certifies that your low income rate is two-thirds or more and— (1) You offer an associate, baccalaureate, graduate, or professional degree, and your completion rate is 70 percent or more; or (2) You do not offer...

  6. 34 CFR 668.194 - Economically disadvantaged appeals.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Rates § 668.194 Economically disadvantaged appeals. (a) Eligibility. As described in this section, you... certifies that your low income rate is two-thirds or more and— (1) You offer an associate, baccalaureate, graduate, or professional degree, and your completion rate is 70 percent or more; or (2) You do not offer...

  7. 34 CFR 668.194 - Economically disadvantaged appeals.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Rates § 668.194 Economically disadvantaged appeals. (a) Eligibility. As described in this section, you... certifies that your low income rate is two-thirds or more and— (1) You offer an associate, baccalaureate, graduate, or professional degree, and your completion rate is 70 percent or more; or (2) You do not offer...

  8. 34 CFR 668.194 - Economically disadvantaged appeals.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Rates § 668.194 Economically disadvantaged appeals. (a) Eligibility. As described in this section, you... certifies that your low income rate is two-thirds or more and— (1) You offer an associate, baccalaureate, graduate, or professional degree, and your completion rate is 70 percent or more; or (2) You do not offer...

  9. 40 CFR 421.194 - Standards of performance for new sources.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 29 2011-07-01 2009-07-01 true Standards of performance for new... Subcategory § 421.194 Standards of performance for new sources. Any new source subject to this subpart shall achieve the following new source performance standards: (a) Displacement Supernatant. NSPS for the...

  10. 40 CFR 421.194 - Standards of performance for new sources.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 29 2014-07-01 2012-07-01 true Standards of performance for new... Subcategory § 421.194 Standards of performance for new sources. Any new source subject to this subpart shall achieve the following new source performance standards: (a) Displacement Supernatant. NSPS for the...

  11. 40 CFR 421.194 - Standards of performance for new sources.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 28 2010-07-01 2010-07-01 true Standards of performance for new... Subcategory § 421.194 Standards of performance for new sources. Any new source subject to this subpart shall achieve the following new source performance standards: (a) Displacement Supernatant. NSPS for the...

  12. 40 CFR 421.194 - Standards of performance for new sources.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 30 2012-07-01 2012-07-01 false Standards of performance for new... Subcategory § 421.194 Standards of performance for new sources. Any new source subject to this subpart shall achieve the following new source performance standards: (a) Displacement Supernatant. NSPS for the...

  13. 40 CFR 421.194 - Standards of performance for new sources.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 30 2013-07-01 2012-07-01 true Standards of performance for new... Subcategory § 421.194 Standards of performance for new sources. Any new source subject to this subpart shall achieve the following new source performance standards: (a) Displacement Supernatant. NSPS for the...

  14. 42 CFR 84.194 - Filters used with chemical cartridges; location; replacement.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false Filters used with chemical cartridges; location... DEVICES Chemical Cartridge Respirators § 84.194 Filters used with chemical cartridges; location; replacement. (a) Particulate matter filters used in conjunction with a chemical cartridge shall be located on...

  15. 42 CFR 84.194 - Filters used with chemical cartridges; location; replacement.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Filters used with chemical cartridges; location... DEVICES Chemical Cartridge Respirators § 84.194 Filters used with chemical cartridges; location; replacement. (a) Particulate matter filters used in conjunction with a chemical cartridge shall be located on...

  16. 42 CFR 84.194 - Filters used with chemical cartridges; location; replacement.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Filters used with chemical cartridges; location... DEVICES Chemical Cartridge Respirators § 84.194 Filters used with chemical cartridges; location; replacement. (a) Particulate matter filters used in conjunction with a chemical cartridge shall be located on...

  17. 42 CFR 84.194 - Filters used with chemical cartridges; location; replacement.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Filters used with chemical cartridges; location... DEVICES Chemical Cartridge Respirators § 84.194 Filters used with chemical cartridges; location; replacement. (a) Particulate matter filters used in conjunction with a chemical cartridge shall be located on...

  18. 30 CFR 19.4 - Conditions governing investigations.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., EVALUATION, AND APPROVAL OF MINING PRODUCTS ELECTRIC CAP LAMPS § 19.4 Conditions governing investigations. (a) One complete lamp, with the assembly and detail drawings that show the construction of the lamp and... lamp has been inspected by MSHA, the applicant will be notified as to the amount of material that will...

  19. 30 CFR 19.4 - Conditions governing investigations.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., EVALUATION, AND APPROVAL OF MINING PRODUCTS ELECTRIC CAP LAMPS § 19.4 Conditions governing investigations. (a) One complete lamp, with the assembly and detail drawings that show the construction of the lamp and... lamp has been inspected by MSHA, the applicant will be notified as to the amount of material that will...

  20. 30 CFR 19.4 - Conditions governing investigations.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., EVALUATION, AND APPROVAL OF MINING PRODUCTS ELECTRIC CAP LAMPS § 19.4 Conditions governing investigations. (a) One complete lamp, with the assembly and detail drawings that show the construction of the lamp and... lamp has been inspected by MSHA, the applicant will be notified as to the amount of material that will...

  1. 42 CFR 84.194 - Filters used with chemical cartridges; location; replacement.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 1 2013-10-01 2013-10-01 false Filters used with chemical cartridges; location... DEVICES Chemical Cartridge Respirators § 84.194 Filters used with chemical cartridges; location; replacement. (a) Particulate matter filters used in conjunction with a chemical cartridge shall be located on...

  2. 46 CFR 194.10-30 - Magazine sprinklers.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... shall be installed in each magazine or magazine group. The control valve shall generally be in... control valve. (2) Sprinkler systems shall be designed in accordance with the requirements of part 76 of..., USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Magazines § 194.10-30 Magazine sprinklers...

  3. 46 CFR 194.10-30 - Magazine sprinklers.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... shall be installed in each magazine or magazine group. The control valve shall generally be in... control valve. (2) Sprinkler systems shall be designed in accordance with the requirements of part 76 of..., USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Magazines § 194.10-30 Magazine sprinklers...

  4. 46 CFR 194.10-30 - Magazine sprinklers.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... shall be installed in each magazine or magazine group. The control valve shall generally be in... control valve. (2) Sprinkler systems shall be designed in accordance with the requirements of part 76 of..., USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Magazines § 194.10-30 Magazine sprinklers...

  5. 46 CFR 194.10-30 - Magazine sprinklers.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... shall be installed in each magazine or magazine group. The control valve shall generally be in... control valve. (2) Sprinkler systems shall be designed in accordance with the requirements of part 76 of..., USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Magazines § 194.10-30 Magazine sprinklers...

  6. 46 CFR 194.10-30 - Magazine sprinklers.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... shall be installed in each magazine or magazine group. The control valve shall generally be in... control valve. (2) Sprinkler systems shall be designed in accordance with the requirements of part 76 of..., USE, AND CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Magazines § 194.10-30 Magazine sprinklers...

  7. 46 CFR 194.10-1 - Application.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... CONTROL OF EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Magazines § 194.10-1 Application. (a) The provisions of this subpart apply to the construction of integral magazines, magazine vans, and magazine chests... construction of magazines shall be in accordance with the applicable provisions of 49 CFR parts 173 and 176 and...

  8. Giftedness and Intuition

    ERIC Educational Resources Information Center

    Karwowski, Maciej

    2008-01-01

    Two studies were conducted to show connections between giftedness and intuition. The first study was exploratory. A sample of 194 gifted adolescent students (N=194) included fifty-five students identified as gifted by their teachers and fifty-six percent who were female. Using the Polish version of the Myers Briggs Type Indicator, the students…

  9. 46 CFR 194.20-1 - General.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... shall be made for the containment and removal of chemical spills. (d) Chemical reactions and experiments... EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemical Stores and/or Storerooms § 194.20-1 General. (a) The chemical storerooms shall be considered to be service areas and as such shall be subject to the applicable...

  10. 46 CFR 194.20-1 - General.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... shall be made for the containment and removal of chemical spills. (d) Chemical reactions and experiments... EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemical Stores and/or Storerooms § 194.20-1 General. (a) The chemical storerooms shall be considered to be service areas and as such shall be subject to the applicable...

  11. 46 CFR 194.20-1 - General.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... shall be made for the containment and removal of chemical spills. (d) Chemical reactions and experiments... EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemical Stores and/or Storerooms § 194.20-1 General. (a) The chemical storerooms shall be considered to be service areas and as such shall be subject to the applicable...

  12. 46 CFR 194.20-1 - General.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... shall be made for the containment and removal of chemical spills. (d) Chemical reactions and experiments... EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemical Stores and/or Storerooms § 194.20-1 General. (a) The chemical storerooms shall be considered to be service areas and as such shall be subject to the applicable...

  13. 46 CFR 194.20-1 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... shall be made for the containment and removal of chemical spills. (d) Chemical reactions and experiments... EXPLOSIVES AND OTHER HAZARDOUS MATERIALS Chemical Stores and/or Storerooms § 194.20-1 General. (a) The chemical storerooms shall be considered to be service areas and as such shall be subject to the applicable...

  14. 49 CFR 194.119 - Submission and approval procedures.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... PLANS FOR ONSHORE OIL PIPELINES Response Plans § 194.119 Submission and approval procedures. (a) Each operator shall submit two copies of the response plan required by this part. Copies of the response plan...: Submission of plans in electronic format is preferred. (b) If PHMSA determines that a response plan requiring...

  15. 49 CFR 194.119 - Submission and approval procedures.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... PLANS FOR ONSHORE OIL PIPELINES Response Plans § 194.119 Submission and approval procedures. (a) Each operator shall submit two copies of the response plan required by this part. Copies of the response plan...: Submission of plans in electronic format is preferred. (b) If PHMSA determines that a response plan requiring...

  16. 49 CFR 194.119 - Submission and approval procedures.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... PLANS FOR ONSHORE OIL PIPELINES Response Plans § 194.119 Submission and approval procedures. (a) Each operator shall submit two copies of the response plan required by this part. Copies of the response plan...: Submission of plans in electronic format is preferred. (b) If PHMSA determines that a response plan requiring...

  17. 49 CFR 194.119 - Submission and approval procedures.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... PLANS FOR ONSHORE OIL PIPELINES Response Plans § 194.119 Submission and approval procedures. (a) Each operator shall submit two copies of the response plan required by this part. Copies of the response plan...: Submission of plans in electronic format is preferred. (b) If PHMSA determines that a response plan requiring...

  18. 49 CFR 194.119 - Submission and approval procedures.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... PLANS FOR ONSHORE OIL PIPELINES Response Plans § 194.119 Submission and approval procedures. (a) Each operator shall submit two copies of the response plan required by this part. Copies of the response plan...: Submission of plans in electronic format is preferred. (b) If PHMSA determines that a response plan requiring...

  19. 40 CFR 194.33 - Consideration of drilling events in performance assessments.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 25 2014-07-01 2014-07-01 false Consideration of drilling events in... and Re-certification Containment Requirements § 194.33 Consideration of drilling events in performance... shall be used in assessing the likelihood and consequences of drilling events, and the results of such...

  20. 40 CFR 194.33 - Consideration of drilling events in performance assessments.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 26 2012-07-01 2011-07-01 true Consideration of drilling events in... and Re-certification Containment Requirements § 194.33 Consideration of drilling events in performance... shall be used in assessing the likelihood and consequences of drilling events, and the results of such...

  1. 40 CFR 194.33 - Consideration of drilling events in performance assessments.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 25 2011-07-01 2011-07-01 false Consideration of drilling events in... and Re-certification Containment Requirements § 194.33 Consideration of drilling events in performance... shall be used in assessing the likelihood and consequences of drilling events, and the results of such...

  2. 40 CFR 194.33 - Consideration of drilling events in performance assessments.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 26 2013-07-01 2013-07-01 false Consideration of drilling events in... and Re-certification Containment Requirements § 194.33 Consideration of drilling events in performance... shall be used in assessing the likelihood and consequences of drilling events, and the results of such...

  3. 29 CFR 19.4 - Contents of request.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Secretary of Labor RIGHT TO FINANCIAL PRIVACY ACT § 19.4 Contents of request. The formal written request shall be in the form of a letter or memorandum to an appropriate official of the financial institution..., business address and business phone number. The request shall also contain the following: (a) The identity...

  4. 30 CFR 250.194 - How must I protect archaeological resources?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 2 2011-07-01 2011-07-01 false How must I protect archaeological resources... SHELF General Information and Reporting Requirements § 250.194 How must I protect archaeological... protect the resource. (c) If you discover any archaeological resource while conducting operations in the...

  5. 40 CFR 194.15 - Content of compliance re-certification application(s).

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 24 2010-07-01 2010-07-01 false Content of compliance re-certification...-certification Applications § 194.15 Content of compliance re-certification application(s). (a) In submitting...; (2) All additional monitoring data, analyses and results; (3) All additional analyses and results of...

  6. Criteria for the Certification and Recertification of the Waste Isolation Pilot Plant's Compliance with the 40 CFR Part 191 Disposal Regulations (40 CFR Part 194)

    EPA Pesticide Factsheets

    EPA is responsible for certifying that DOE’s Waste Isolation Pilot Plant (WIPP) remains in compliance with environmental standards for the disposal of transuranic waste. 40 CFR Part 194 specifies criteria for certification or recertification of WIPP.

  7. 38 CFR 17.194 - Aid for domiciliary care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Aid for domiciliary care... Aid to States for Care of Veterans in State Homes § 17.194 Aid for domiciliary care. Aid may be paid to the designated State official for domiciliary care furnished in a recognized State home for any...

  8. 12 CFR 19.194 - Eligibility of attorneys and accountants to practice.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... certified public accountant or public accountant and is not currently under suspension or debarment by the... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Eligibility of attorneys and accountants to... Conduct § 19.194 Eligibility of attorneys and accountants to practice. (a) Attorneys. Any attorney who is...

  9. 27 CFR 41.194 - Articles of partnership or association.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2013-04-01 2013-04-01 false Articles of partnership or... AND TUBES, AND PROCESSED TOBACCO Tobacco Products Importers § 41.194 Articles of partnership or..., must furnish with its application for permit required by § 41.191 a true copy of the articles of...

  10. 27 CFR 41.194 - Articles of partnership or association.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2014-04-01 2014-04-01 false Articles of partnership or... AND TUBES, AND PROCESSED TOBACCO Tobacco Products Importers § 41.194 Articles of partnership or... products must furnish with its application for the permit required by § 41.191 a true copy of the articles...

  11. 27 CFR 41.194 - Articles of partnership or association.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2011-04-01 2011-04-01 false Articles of partnership or... AND TUBES, AND PROCESSED TOBACCO Tobacco Products Importers § 41.194 Articles of partnership or..., must furnish with its application for permit required by § 41.191 a true copy of the articles of...

  12. 194. Photocopy of drawing, Twin Falls Canal Company, date unknown. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    194. Photocopy of drawing, Twin Falls Canal Company, date unknown. PROFILE AND GATE PLAN, NORTH ISLAND CROSS SECTION OF DAM, TWIN FALLS COUNTY, MILNER, IDAHO; BLUEPRINT. - Milner Dam & Main Canal: Twin Falls Canal Company, On Snake River, 11 miles West of city of Burley, Idaho, Twin Falls, Twin Falls County, ID

  13. Notes on LCW Activation Calculation for Neutron Imaging Operations in the North Cave of Building 194

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Anderson, S. G.

    This note estimates the amount of activation that could be produced in the Facilities-provided Low Conductivity Water (LCW) that is proposed to be used for cooling of electromagnets and beam stops in the Neutron Imaging (NI) accelerator project in the North Cave of Building 194.

  14. 46 CFR 194.20-15 - Chemical stores other than compressed gases.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Storerooms § 194.20-15 Chemical stores other than compressed gases. (a) Flammable liquids are excluded from the storeroom unless contained in properly marked and labeled metal safety cans not in excess of 5... than 5 gallons. (b) Combustible liquids in approved portable drums, barrels or containers not in excess...

  15. 46 CFR 194.20-15 - Chemical stores other than compressed gases.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Storerooms § 194.20-15 Chemical stores other than compressed gases. (a) Flammable liquids are excluded from the storeroom unless contained in properly marked and labeled metal safety cans not in excess of 5... than 5 gallons. (b) Combustible liquids in approved portable drums, barrels or containers not in excess...

  16. 46 CFR 194.20-15 - Chemical stores other than compressed gases.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Storerooms § 194.20-15 Chemical stores other than compressed gases. (a) Flammable liquids are excluded from the storeroom unless contained in properly marked and labeled metal safety cans not in excess of 5... than 5 gallons. (b) Combustible liquids in approved portable drums, barrels or containers not in excess...

  17. 46 CFR 194.20-15 - Chemical stores other than compressed gases.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Storerooms § 194.20-15 Chemical stores other than compressed gases. (a) Flammable liquids are excluded from the storeroom unless contained in properly marked and labeled metal safety cans not in excess of 5... than 5 gallons. (b) Combustible liquids in approved portable drums, barrels or containers not in excess...

  18. 46 CFR 194.20-15 - Chemical stores other than compressed gases.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Storerooms § 194.20-15 Chemical stores other than compressed gases. (a) Flammable liquids are excluded from the storeroom unless contained in properly marked and labeled metal safety cans not in excess of 5... than 5 gallons. (b) Combustible liquids in approved portable drums, barrels or containers not in excess...

  19. 46 CFR 194.05-5 - Chemicals in the chemistry laboratory.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Chemicals in the chemistry laboratory. 194.05-5 Section....05-5 Chemicals in the chemistry laboratory. (a) Small working quantities of chemical stores in the chemistry laboratory which have been removed from the approved shipping container need not be marked or...

  20. 27 CFR 41.194 - Articles of partnership or association.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Articles of partnership or... AND TUBES, AND PROCESSED TOBACCO Tobacco Products Importers § 41.194 Articles of partnership or..., must furnish with its application for permit required by § 41.191 a true copy of the articles of...

  1. 46 CFR 194.05-5 - Chemicals in the chemistry laboratory.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Chemicals in the chemistry laboratory. 194.05-5 Section....05-5 Chemicals in the chemistry laboratory. (a) Small working quantities of chemical stores in the chemistry laboratory which have been removed from the approved shipping container need not be marked or...

  2. 46 CFR 194.05-5 - Chemicals in the chemistry laboratory.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Chemicals in the chemistry laboratory. 194.05-5 Section....05-5 Chemicals in the chemistry laboratory. (a) Small working quantities of chemical stores in the chemistry laboratory which have been removed from the approved shipping container need not be marked or...

  3. 46 CFR 194.05-5 - Chemicals in the chemistry laboratory.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Chemicals in the chemistry laboratory. 194.05-5 Section....05-5 Chemicals in the chemistry laboratory. (a) Small working quantities of chemical stores in the chemistry laboratory which have been removed from the approved shipping container need not be marked or...

  4. 46 CFR 194.05-5 - Chemicals in the chemistry laboratory.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Chemicals in the chemistry laboratory. 194.05-5 Section....05-5 Chemicals in the chemistry laboratory. (a) Small working quantities of chemical stores in the chemistry laboratory which have been removed from the approved shipping container need not be marked or...

  5. 38 CFR 21.194 - “Employment services” status.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    .... Chapter 31 Case Status § 21.194 “Employment services” status. (a) Purpose. The status employment services... veteran's case may be assigned or reassigned to employment services status under the provisions of §§ 21.84, 21.88, 21.94 and 21.98. (c) Continuation in employment services status. A case will remain in...

  6. 43 CFR 19.4 - Liaison with other governmental agencies and submission of views by interested persons.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... National Wildlife Refuge System, may submit such recommendations at any time to the superintendent or... Secretary of the Interior WILDERNESS PRESERVATION National Wilderness Preservation System § 19.4 Liaison... recommendations as to the suitability or nonsuitability for preservation as wilderness of any roadless area in any...

  7. 30 CFR 550.194 - How must I protect archaeological resources?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false How must I protect archaeological resources... Reporting Requirements § 550.194 How must I protect archaeological resources? (a) If the Regional Director... until the Regional Director has told you how to protect the resource. (c) If you discover any...

  8. 30 CFR 550.194 - How must I protect archaeological resources?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 2 2014-07-01 2014-07-01 false How must I protect archaeological resources... Reporting Requirements § 550.194 How must I protect archaeological resources? (a) If the Regional Director... until the Regional Director has told you how to protect the resource. (c) If you discover any...

  9. 30 CFR 250.194 - How must I protect archaeological resources?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false How must I protect archaeological resources... Reporting Requirements § 250.194 How must I protect archaeological resources? (a) If the Regional Director... until the Regional Director has told you how to protect the resource. (c) If you discover any...

  10. 30 CFR 550.194 - How must I protect archaeological resources?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 2 2012-07-01 2012-07-01 false How must I protect archaeological resources... Reporting Requirements § 550.194 How must I protect archaeological resources? (a) If the Regional Director... until the Regional Director has told you how to protect the resource. (c) If you discover any...

  11. 12 CFR 19.194 - Eligibility of attorneys and accountants to practice.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 1 2011-01-01 2011-01-01 false Eligibility of attorneys and accountants to... Conduct § 19.194 Eligibility of attorneys and accountants to practice. (a) Attorneys. Any attorney who is... subpart may practice before the OCC. (b) Accountants. Any accountant who is qualified to practice as a...

  12. 12 CFR 19.194 - Eligibility of attorneys and accountants to practice.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 1 2012-01-01 2012-01-01 false Eligibility of attorneys and accountants to... Conduct § 19.194 Eligibility of attorneys and accountants to practice. (a) Attorneys. Any attorney who is... subpart may practice before the OCC. (b) Accountants. Any accountant who is qualified to practice as a...

  13. 12 CFR 19.194 - Eligibility of attorneys and accountants to practice.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 1 2013-01-01 2013-01-01 false Eligibility of attorneys and accountants to... Conduct § 19.194 Eligibility of attorneys and accountants to practice. (a) Attorneys. Any attorney who is... subpart may practice before the OCC. (b) Accountants. Any accountant who is qualified to practice as a...

  14. 12 CFR 19.194 - Eligibility of attorneys and accountants to practice.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 1 2014-01-01 2014-01-01 false Eligibility of attorneys and accountants to... Conduct § 19.194 Eligibility of attorneys and accountants to practice. (a) Attorneys. Any attorney who is... subpart may practice before the OCC. (b) Accountants. Any accountant who is qualified to practice as a...

  15. Short-lived isomers in 192Po and 194Po

    NASA Astrophysics Data System (ADS)

    Andel, B.; Andreyev, A. N.; Antalic, S.; Heßberger, F. P.; Ackermann, D.; Hofmann, S.; Huyse, M.; Kalaninová, Z.; Kindler, B.; Kojouharov, I.; Kuusiniemi, P.; Lommel, B.; Nishio, K.; Page, R. D.; Sulignano, B.; Van Duppen, P.

    2016-06-01

    Isomeric states in 194Po and 192Po were studied at the velocity filter SHIP. The isotopes were produced in the fusion-evaporation reactions 141Pr(56Fe, p 2 n )194Po and 144Sm(51V, p 2 n )192Po . Several new γ -ray transitions were attributed to the isomers and γ -γ coincidences for both isomers were studied for the first time. The 459-keV transition earlier, tentatively proposed as de-exciting the isomeric level in 194Po, was replaced by a new 248-keV transition, and the spin of this isomer was reassigned from (11-) to (10-). The de-excitation of the (11-) isomeric level in 192Po by the 154-keV transition was confirmed and a parallel de-excitation by a 733-keV (E 3 ) transition to (8+) level of the ground-state band was suggested. Moreover, side feeding to the (4+) level of the ground-state band was proposed. The paper also discusses strengths of transitions de-exciting 11- isomers in neighboring Po and Pb isotopes.

  16. Functional contribution of coenzyme specificity-determining sites of 7α-hydroxysteroid dehydrogenase from Clostridium absonum.

    PubMed

    Lou, Deshuai; Wang, Yue; Tan, Jun; Zhu, Liancai; Ji, Shunlin; Wang, Bochu

    2017-10-01

    Studies of the molecular determinants of coenzyme specificity help to reveal the structure-function relationship of enzymes, especially with regards to coenzyme specificity-determining sites (CSDSs) that usually mediate complex interactions. NADP(H)-dependent 7α-hydroxysteroid dehydrogenase from Clostridium absonum (CA 7α-HSDH), a member of the short-chain dehydrogenase/reductase superfamily (SDRs), possesses positively charged CSDSs that mainly contain T15, R16, R38, and R194, forming complicated polar interactions with the adenosine ribose C2 phosphate group of NADP(H). The R38 residue is crucial for coenzyme anchoring, but the influence of the other residues on coenzyme utilization is still not clear. Hence, we performed alanine scanning mutagenesis and molecular dynamic (MD) simulations. The results suggest that the natural CSDSs have the greatest NADP(H)-binding affinity, but not the best activity (k cat ) toward NADP + . Compared with the wild type and other mutants, the mutant R194A showed the highest catalytic efficiency (k cat /K m ), which was more than three-times that of the wild type. MD simulation and kinetics analysis suggested that the importance of the CSDSs of CA 7α-HSDH should be in accordance with the following order R38>T15>R16>R194, and S39 may have a supporting role in NADP(H) anchoring for mutants R16A/T194A and T15A/R16A/T194A. Copyright © 2017. Published by Elsevier Ltd.

  17. Generation of continuous-wave 194 nm laser for mercury ion optical frequency standard

    NASA Astrophysics Data System (ADS)

    Zou, Hongxin; Wu, Yue; Chen, Guozhu; Shen, Yong; Liu, Qu; Precision measurement; atomic clock Team

    2015-05-01

    194 nm continuous-wave (CW) laser is an essential part in mercury ion optical frequency standard. The continuous-wave tunable radiation sources in the deep ultraviolet (DUV) region of the spectrum is also serviceable in high-resolution spectroscopy with many atomic and molecular lines. We introduce a scheme to generate continuous-wave 194 nm radiation with SFM in a Beta Barium Borate (BBO) crystal here. The two source beams are at 718 nm and 266 nm, respectively. Due to the property of BBO, critical phase matching (CPM) is implemented. One bow-tie cavity is used to resonantly enhance the 718 nm beam while the 266 nm makes a single pass, which makes the configuration easy to implement. Considering the walk-off effect in CPM, the cavity mode is designed to be elliptical so that the conversion efficiency can be promoted. Since the 266 nm radiation is generated by a 532 nm laser through SHG in a BBO crystal with a large walk-off angle, the output mode is quite non-Gaussian. To improve mode matching, we shaped the 266 nm beam into Gaussian modes with a cylindrical lens and iris diaphragm. As a result, 2.05 mW 194 nm radiation can be generated. As we know, this is the highest power for 194 nm CW laser using SFM in BBO with just single resonance. The work is supported by the National Natural Science Foundation of China (Grant No. 91436103 and No. 11204374).

  18. Characterization of CDOM of river waters in China using fluorescence excitation-emission matrix and regional integration techniques

    NASA Astrophysics Data System (ADS)

    Zhao, Ying; Song, Kaishan; Shang, Yingxin; Shao, Tiantian; Wen, Zhidan; Lv, Lili

    2017-08-01

    The spatial characteristics of fluorescent dissolved organic matter (FDOM) components in river waters in China were first examined by excitation-emission matrix spectra and fluorescence regional integration (FRI) with the data collected during September to November between 2013 and 2015. One tyrosine-like (R1), one tryptophan-like (R2), one fulvic-like (R3), one microbial protein-like (R4), and one humic-like (R5) components have been identified by FRI method. Principal component analysis (PCA) was conducted to assess variations in the five FDOM components (FRί (ί = 1, 2, 3, 4, and 5)) and the humification index for all 194 river water samples. The average fluorescence intensities of the five fluorescent components and the total fluorescence intensities FSUM differed under spatial variation among the seven major river basins (Songhua, Liao, Hai, Yellow and Huai, Yangtze, Pearl, and Inflow Rivers) in China. When all the river water samples were pooled together, the fulvic-like FR3 and the humic-like FR5 showed a strong positive linear relationship (R2 = 0.90, n = 194), indicating that the two allochthonous FDOM components R3 and R5 may originate from similar sources. There is a moderate strong positive correlation between the tryptophan-like FR2 and the microbial protein-like FR4 (R2 = 0.71, n = 194), suggesting that parts of two autochthonous FDOM components R2 and R4 are likely from some common sources. However, the total allochthonous substance FR(3+5) and the total autochthonous substances FR(1+2+4) exhibited a weak correlation (R2 = 0.40, n = 194). Significant positive linear relationships between FR3 (R2 = 0.69, n = 194), FR5 (R2 = 0.79, n = 194), and chromophoric DOM (CDOM) absorption coefficient a(254) were observed, which demonstrated that the CDOM absorption was dominated by the allochthonous FDOM components R3 and R5.

  19. The Caenorhabditis elegans SH2 domain-containing protein tyrosine phosphatase PTP-2 participates in signal transduction during oogenesis and vulval development

    PubMed Central

    Gutch, Michael J.; Flint, Andrew J.; Keller, James; Tonks, Nicholas K.; Hengartner, Michael O.

    1998-01-01

    Src homology-2 (SH2) domain-containing protein tyrosine phosphatases (SHPs) have been identified as either positive or negative regulators of signaling events downstream of receptor protein tyrosine kinases (R-PTKs). We describe here our characterization of ptp-2, a Caenorhabditis elegans gene that encodes a 668-amino-acid SHP. We isolated a recessive ptp-2 loss-of-function allele, op194, that lacks the conserved protein tyrosine phosphatase catalytic domain by screening for transposon-mediated deletion mutations. Homozygous ptp-2(op194) hermaphrodites exhibit a completely penetrant zygotic semisterile/maternal effect lethal phenotype, characterized by the presence of abnormally large oocytes in the zygotic semisterile animals. These phenotypes indicate that PTP-2 activity is essential for proper oogenesis. Gain-of-function let-60 ras alleles rescued the defects associated with ptp-2(op194), suggesting that LET-60 Ras acts downstream of, or in parallel to, PTP-2 during oogenesis. Although ptp-2 function is not required for normal vulval development, ptp-2(op194) altered significantly the vulval phenotypes caused by mutations in several genes of the inductive signaling pathway. The penetrance of the multivulva phenotype caused by loss-of-function mutations in lin-15, and gain-of-function mutations in let-23 or let-60 ras, was reduced by ptp-2(op194). Moreover, ptp-2(op194) increased the penetrance of the vulvaless phenotype conferred by a weak loss-of-function sem-5 allele. Taken together, our genetic data positions PTP-2 activity downstream of LET-23 in the vulval induction signaling pathway. Although PTP-2 functions to transmit a requisite signal during oogenesis, PTP-2 function during C. elegans vulval cell differentiation appears to be directed at regulating the overall strength of the inductive signal, which may contribute to the quantitative differences in signaling required for the proper specification of the 1°, 2°, and 3° vulval cell fates. PMID:9472025

  20. Structure of the human sulfhydryl oxidase augmenter of liver regeneration and characterization of a human mutation causing an autosomal recessive myopathy†, ‡

    PubMed Central

    Daithankar, Vidyadhar N.; Schaefer, Stephanie A.; Dong, Ming; Bahnson, Brian J.; Thorpe, Colin

    2010-01-01

    The sulfhydryl oxidase augmenter of liver regeneration (ALR) binds FAD in a helix-rich domain that presents a CxxC disulfide proximal to the isoalloxazine ring of the flavin. Head-to-tail interchain disulfide bonds link subunits within the homodimer of both the short, cytokine-like, form of ALR (sfALR), and a longer form (lfALR) which resides in the mitochondrial intermembrane space (IMS). lfALR has an 80-residue N-terminal extension with an additional CxxC motif required for the reoxidation of reduced Mia40 during oxidative protein folding within the IMS. Recently Di Fonzo et al. (Di Fonzo, A., Ronchi, D., Lodi, T., Fassone, E., Tigano, M., Lamperti, C., Corti, S., Bordoni, A., Fortunato, F., Nizzardo, M., Napoli, L., Donadoni, C., Salani, S., Saladino, F., Moggio, M., Bresolin, N., Ferrero, I., and Comi, G. P. (2009) Am. J. Hum. Genet. 84, 594–604) described an R194H mutation of human ALR that led to cataract, progressive muscle hypotonia, and hearing loss in three children. The current work presents a structural and enzymological characterization of the human R194H mutant in lf- and sfALR. A crystal structure of human sfALR was determined by molecular replacement using the rat sfALR structure. R194 is located at the subunit interface of sfALR, close to the intersubunit disulfide bridges. The R194 guanidino moiety participates in three H-bonds: two main-chain carbonyl oxygen atoms (from R194 itself, and from C95 of the intersubunit disulfide of the other protomer) and with the 2' OH of the FAD ribose. The R194H mutation has minimal effect on the enzyme activity using model and physiological substrates of short and long ALR forms. However the mutation adversely affects the stability of both ALR forms: e.g. by decreasing the melting temperature by about 10 °C, by increasing the rate of dissociation of FAD from the holoenzyme by about 45-fold, and by strongly enhancing the susceptibility of sfALR to partial proteolysis and to reduction of its intersubunit disulfide bridges by glutathione. Finally, a comparison of the TROSY-HSQC 2D NMR spectra of wild type sfALR and its R194H mutant reveal a significant increase in conformational flexibility in the mutant protein. In sum, these in vitro data document the major impact of the seemingly conservative R194H mutation on the stability of dimeric ALR and complement the in vivo observations of Di Fonzo et al. PMID:20593814

  1. Lead and eagles: demographic and pathological characteristics of poisoning, and exposure levels associated with other causes of mortality

    USGS Publications Warehouse

    Franson, J. Christian; Russell, Robin E.

    2014-01-01

    We conducted a retrospective analysis to evaluate demographic and pathologic characteristics in 484 bald eagles (Haliaeetus leucocephalus) and 68 golden eagles (Aquila chrysaetos) diagnosed with lead poisoning at the U.S. Geological Survey National Wildlife Health Center. As part of our analysis, we compared characteristics of lead poisoned eagles with those that died of other causes. Odds of lead poisoning were greater for bald eagles versus golden eagles, females versus males, adults versus juveniles, and eagles from the Mississippi and Central flyways versus the Atlantic and Pacific flyways. In addition to spatial, species, and demographic associations, we detected a distinct temporal trend in the collection date of lead poisoned bald eagle carcasses. These carcasses were found at greater frequency in late autumn and winter than spring and summer. Lesions in lead poisoned birds included emaciation, evidence of bile stasis, myocardial degeneration and necrosis, and renal tubular nephrosis and necrosis. Ingested lead ammunition or fragments were found in 14.2 % of bald eagles and 11.8 % of golden eagles. The overall mean liver lead concentration (wet weight basis) for eagles diagnosed with lead poisoning was 28.9 ± 0.69 SE mg/kg in bald eagles and 19.4 ± 1.84 SE mg/kg in golden eagles. In eagles diagnosed with collision trauma, electrocution, poisoning (other than lead), emaciation, infectious disease, trapping death, other, and undetermined causes, average liver lead concentrations were low (<1 mg/kg) and did not differ among causes of mortality. Thus, based on our data, we found no evidence that lead exposure of eagles predisposed them to other causes of mortality.

  2. Enclosure (from letter from EPA to DOE sent 3/26/04) - Technical and Regulatory Support Document: EPA's Remote Handled Waste Characterization Determination

    EPA Pesticide Factsheets

    Based on the requirements presented in 40 CFR 194.24(c )(2) to (4) and 194.22(a)(1) and using experience gained as part of the CH waste characterization program, EPA examined the DOE's RH Waste Characterization Proposal as presented in the WCPIP.

  3. Effects of inoculation of commercial starter cultures on the quality and histamine accumulation in fermented sausages.

    PubMed

    Wang, Xinhui; Ren, Hongyang; Wang, Wei; Zhang, Yin; Bai, Ting; Li, Junxia; Zhu, Wenyou

    2015-02-01

    To meet the requirements of high-quality safe products, starter cultures are used to produce fermented sausages. The effects of 3 commercial starter cultures, namely SM-194, T-SPX, and SM-181, on histamine accumulation and quality parameters including microbial quality, pH, water activity, and total volatile base nitrogen, as well as the color and texture properties, were evaluated during the fermentation and ripening of fermented sausages. Although initial counts of Escherichia coli, Enterobacteriaceae, and Pseudomonas were similar in the 4 batches, the growth of these microorganisms was significantly inhibited (P < 0.05) in batches SM-194, T-SPX, and SM-181 throughout the fermentation and ripening period. The counts of E. coli, Enterobacteriaceae, and Pseudomonas increased to maximum levels of 3.89, 4.41, and 5.15 log10 colony forming units/g in the control sausages, respectively. At the end of ripening, the levels of histamine were 8.85, 0.32, 7.82, and 3.18 mg/kg for batches C, SM-194, T-SPX, and SM-181, respectively. The results revealed that commercial starter cultures, particularly starter cultures SM-194 and SM-181, made a great contribution to histamine reduction. In addition, batches inoculated with starter cultures showed a stronger acidification and lower level of total volatile base nitrogen than the control sample during production (P < 0.05). In conclusion, it seems that the inoculation of commercial starter cultures, particularly starter cultures SM-194 and SM-181, contributes to improving microbial quality, hygienic quality and food safety of fermented sausages. © 2015 Institute of Food Technologists®

  4. Association of tracheal mucus or blood and airway neutrophilia with racing performance in Thoroughbred horses in an Australian racing yard.

    PubMed

    Salz, R O; Ahern, B J; Boston, R; Begg, L M

    2016-04-01

    To determine the variation of tracheal mucus scores, tracheal blood scores and transendoscopic tracheal wash (TW) cytology in a population of Thoroughbred (TB) racehorses and assess their association with racing performance. A total of 220 endoscopic examinations were performed and TWs obtained from 155 TB racehorses. Samples were collected 60-120 min following gallop work. Tracheal mucus score, tracheal blood score and TW cytology were analysed and their association with racing performance assessed. Of the total examinations and samples, 194 from 135 horses fitted the criteria for inclusion. The overall prevalence of visible tracheal mucus was 2.5% (5/194) and of increased tracheal mucus was 0%. The prevalence of visible tracheal blood was 8.8% (17/194) and of increased tracheal blood was 4.6% (9/194). A total of 36% (70/194) of TWs contained elevated percentages of neutrophils and of these, 96% (67/70) occurred in the absence of any visible tracheal mucus. There was no significant association between tracheal mucus score or TW cytology and subsequent racing performance. There was a statistically significant association (P = 0.004) between increased tracheal blood scores and poor racing performance. Visible tracheal blood seen after strenuous exercise in clinically normal TB racehorses was a risk factor for poor racing performance, but the presence of airway neutrophilia was not. No horses in this study were found to have increased tracheal mucus, so the association of increased tracheal mucus with racing performance could not be assessed. © 2016 Australian Veterinary Association.

  5. Decommissioning and PIE of the MEGAPIE spallation target

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Latge, C.; Henry, J.; Wohlmuther, M.

    2013-07-01

    A key experiment in the Accelerated Driven Systems roadmap, the MEGAwatt PIlot Experiment (MEGAPIE) (1 MW) was initiated in 1999 in order to design and build a liquid lead-bismuth spallation target, then to operate it into the Swiss spallation neutron facility SINQ at Paul Scherrer Institute. The target has been designed, manufactured, and tested during integral tests, before irradiation carried out end of 2006. During irradiation, neutron and thermo hydraulic measurements were performed allowing deep interpretation of the experiment and validation of the models used during design phase. The decommissioning, Post Irradiation Examinations and waste management phases were defined properly.more » The phases dedicated to cutting, sampling, cleaning, waste management, samples preparation and shipping to various laboratories were performed by PSI teams: all these phases constitute a huge work, which allows now to perform post-irradiation examination (PIE) of structural material, irradiated in relevant conditions. Preliminary results are presented in the paper, they concern chemical characterization. The following radio-nuclides have been identified by γ-spectrometry: {sup 60}Co, {sup 101}Rh, {sup 102}Rh, {sup 108m}Ag, {sup 110m}Ag, {sup 133}Ba, {sup 172}Hf/Lu, {sup 173}Lu, {sup 194}Hg/Au, {sup 195}Au, {sup 207}Bi. For some of these nuclides the activities can be easily evaluated from γ-spectrometry results ({sup 207}Bi, {sup 194}Hg/Au), while other nuclides can only be determined after chemical separations ({sup 108m}Ag, {sup 110m}Ag, {sup 195}Au, {sup 129}I, {sup 36}Cl and α-emitting {sup 208-210}Po). The concentration of {sup 129}I is lower than expected. The chemical analysis already performed on spallation and corrosion products in the lead-bismuth eutectic (LBE) are very relevant for further applications of LBE as a spallation media and more generally as a coolant.« less

  6. Clinical safety of the ProMRI pacemaker system in patients subjected to thoracic spine and cardiac 1.5-T magnetic resonance imaging scanning conditions.

    PubMed

    Bailey, William M; Mazur, Alexander; McCotter, Craig; Woodard, Pamela K; Rosenthal, Lawrence; Johnson, Whitney; Mela, Theofanie

    2016-02-01

    Permanent cardiac pacemakers have historically been considered a contraindication to magnetic resonance imaging (MRI). The purpose of the ProMRI Phase B Study, a multicenter, prospective, single-arm, nonrandomized study, was to evaluate the clinical safety of the Biotronik ProMRI pacemaker system in patients undergoing thoracic spine and cardiac MRI. The ProMRI Phase B study enrolled 245 patients with stable baseline pacing indices implanted with an Entovis pacemaker (DR-T or SR-T) and Setrox 53-cm and/or 60-cm lead(s). Device interrogation was performed at enrollment, pre- and post-MRI scan, and 1 and 3 months post-MRI. End-points were (1) freedom from MRI- and pacing system-related serious adverse device effects through 1 month post-MRI; (2) freedom from atrial and ventricular MRI-induced pacing threshold increase (>0.5 V); and (3) freedom from P- and R-wave amplitude attenuation (<50%), or P wave <1.5 mV, or R wave <5.0 mV at 1 month post-MRI. In total, 216 patients completed the MRI and 1-month post-MRI follow-up. One adverse event possibly related to the implanted system and the MRI procedure occurred, resulting in a serious adverse device effect-free rate of 99.6% (220/221; P < .0001. Freedom from atrial and ventricular pacing threshold increase was 100% (194/194, P < .001) and 100% (206/206, P < .001) respectively. Freedom from P- and R-wave amplitude attenuation was 98.2% (167/170, P < .001) and 100% (188/188, P < .001) respectively. The results of the ProMRI Phase B study demonstrate the clinical safety and efficacy of the ProMRI pacemaker system in patients subjected to thoracic spine and cardiac MRI conditions. Copyright © 2016 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.

  7. China Report, Agriculture, 1983 Agricultural Yearbook

    DTIC Science & Technology

    1986-07-24

    River Network Regions 147 Responsibility Systems of Grassland Management and Cattle Management Should Be Suitable to Each Other 149 Contract...Its Cultivation w 192 Animal Husbandry t 194 Develop the Production of Milk Cattle 194 / Carefully Attend to the Improvement of Domestic Animals...Combination of Animal Husbandry and Fishery in River-Network Regions 219 Responsibility Systems of Grassland Management and Cattle Management Should

  8. Proline isomerization in the C-terminal region of HSP27.

    PubMed

    Alderson, T Reid; Benesch, Justin L P; Baldwin, Andrew J

    2017-07-01

    In mammals, small heat-shock proteins (sHSPs) typically assemble into interconverting, polydisperse oligomers. The dynamic exchange of sHSP oligomers is regulated, at least in part, by molecular interactions between the α-crystallin domain and the C-terminal region (CTR). Here we report solution-state nuclear magnetic resonance (NMR) spectroscopy investigations of the conformation and dynamics of the disordered and flexible CTR of human HSP27, a systemically expressed sHSP. We observed multiple NMR signals for residues in the vicinity of proline 194, and we determined that, while all observed forms are highly disordered, the extra resonances arise from cis-trans peptidyl-prolyl isomerization about the G193-P194 peptide bond. The cis-P194 state is populated to near 15% at physiological temperatures, and, although both cis- and trans-P194 forms of the CTR are flexible and dynamic, both states show a residual but differing tendency to adopt β-strand conformations. In NMR spectra of an isolated CTR peptide, we observed similar evidence for isomerization involving proline 182, found within the IPI/V motif. Collectively, these data indicate a potential role for cis-trans proline isomerization in regulating the oligomerization of sHSPs.

  9. NS1 Protein Amino Acid Changes D189N and V194I Affect Interferon Responses, Thermosensitivity, and Virulence of Circulating H3N2 Human Influenza A Viruses

    PubMed Central

    Nogales, Aitor; Martinez-Sobrido, Luis

    2016-01-01

    ABSTRACT Influenza virus NS1 protein is a nonstructural, multifunctional protein that counteracts host innate immune responses, modulating virus pathogenesis. NS1 protein variability in subjects infected with H3N2 influenza A viruses (IAVs) during the 2010/2011 season was analyzed, and amino acid changes in residues 86, 189, and 194 were found. The consequences of these mutations for the NS1-mediated inhibition of IFN responses and the pathogenesis of the virus were evaluated, showing that NS1 mutations D189N and V194I impaired the ability of the NS1 protein to inhibit general gene expression, most probably because these mutations decreased the binding of NS1 to the cleavage and polyadenylation specificity factor 30 (CPSF30). A recombinant A/Puerto Rico/8/34 (PR8) H1N1 virus encoding the H3N2 NS1-D189N protein was slightly attenuated, whereas the virus encoding the H3N2 NS1-V194I protein was further attenuated in mice. The higher attenuation of this virus could not be explained by differences in the ability of the two NS1 proteins to counteract host innate immune responses, indicating that another factor must be responsible. In fact, we showed that the virus encoding the H3N2 NS1-V194I protein demonstrated a temperature-sensitive (ts) phenotype, providing a most likely explanation for the stronger attenuation observed. As far as we know, this is the first description of a mutation in NS1 residue 194 conferring a ts phenotype. These studies are relevant in order to identify new residues important for NS1 functions and in human influenza virus surveillance to assess mutations affecting the pathogenicity of circulating viruses. IMPORTANCE Influenza viral infections represent a serious public health problem, with influenza virus causing a contagious respiratory disease that is most effectively prevented through vaccination. The multifunctional nonstructural protein 1 (NS1) is the main viral factor counteracting the host antiviral response. Therefore, influenza virus surveillance to identify new mutations in the NS1 protein affecting the pathogenicity of the circulating viruses is highly important. In this work, we evaluated amino acid variability in the NS1 proteins from H3N2 human seasonal viruses and the effect of the mutations on innate immune responses and virus pathogenesis. NS1 mutations D189N and V194I impaired the ability of the NS1 protein to inhibit general gene expression, and recombinant viruses harboring these mutations were attenuated in a mouse model of influenza infection. Interestingly, a virus encoding the H3N2 NS1-V194I protein demonstrated a temperature-sensitive phenotype, further attenuating the virus in vivo. PMID:28003482

  10. NS1 Protein Amino Acid Changes D189N and V194I Affect Interferon Responses, Thermosensitivity, and Virulence of Circulating H3N2 Human Influenza A Viruses.

    PubMed

    Nogales, Aitor; Martinez-Sobrido, Luis; Topham, David J; DeDiego, Marta L

    2017-03-01

    Influenza virus NS1 protein is a nonstructural, multifunctional protein that counteracts host innate immune responses, modulating virus pathogenesis. NS1 protein variability in subjects infected with H3N2 influenza A viruses (IAVs) during the 2010/2011 season was analyzed, and amino acid changes in residues 86, 189, and 194 were found. The consequences of these mutations for the NS1-mediated inhibition of IFN responses and the pathogenesis of the virus were evaluated, showing that NS1 mutations D189N and V194I impaired the ability of the NS1 protein to inhibit general gene expression, most probably because these mutations decreased the binding of NS1 to the cleavage and polyadenylation specificity factor 30 (CPSF30). A recombinant A/Puerto Rico/8/34 (PR8) H1N1 virus encoding the H3N2 NS1-D189N protein was slightly attenuated, whereas the virus encoding the H3N2 NS1-V194I protein was further attenuated in mice. The higher attenuation of this virus could not be explained by differences in the ability of the two NS1 proteins to counteract host innate immune responses, indicating that another factor must be responsible. In fact, we showed that the virus encoding the H3N2 NS1-V194I protein demonstrated a temperature-sensitive (ts) phenotype, providing a most likely explanation for the stronger attenuation observed. As far as we know, this is the first description of a mutation in NS1 residue 194 conferring a ts phenotype. These studies are relevant in order to identify new residues important for NS1 functions and in human influenza virus surveillance to assess mutations affecting the pathogenicity of circulating viruses. IMPORTANCE Influenza viral infections represent a serious public health problem, with influenza virus causing a contagious respiratory disease that is most effectively prevented through vaccination. The multifunctional nonstructural protein 1 (NS1) is the main viral factor counteracting the host antiviral response. Therefore, influenza virus surveillance to identify new mutations in the NS1 protein affecting the pathogenicity of the circulating viruses is highly important. In this work, we evaluated amino acid variability in the NS1 proteins from H3N2 human seasonal viruses and the effect of the mutations on innate immune responses and virus pathogenesis. NS1 mutations D189N and V194I impaired the ability of the NS1 protein to inhibit general gene expression, and recombinant viruses harboring these mutations were attenuated in a mouse model of influenza infection. Interestingly, a virus encoding the H3N2 NS1-V194I protein demonstrated a temperature-sensitive phenotype, further attenuating the virus in vivo . Copyright © 2017 American Society for Microbiology.

  11. DNA Repair Mechanism Gene, XRCC1A ( Arg194Trp) but not XRCC3 ( Thr241Met) Polymorphism Increased the Risk of Breast Cancer in Premenopausal Females: A Case-Control Study in Northeastern Region of India.

    PubMed

    Devi, K Rekha; Ahmed, Jishan; Narain, Kanwar; Mukherjee, Kaustab; Majumdar, Gautam; Chenkual, Saia; Zonunmawia, Jason C

    2017-12-01

    X-ray repair cross complementary group gene is one of the most studied candidate gene involved in different types of cancers. Studies have shown that X-ray repair cross complementary genes are significantly associated with increased risk of breast cancer in females. Moreover, studies have revealed that X-ray repair cross complementary gene polymorphism significantly varies between and within different ethnic groups globally. The present case-control study was aimed to investigate the association of X-ray repair cross complementary 1A (Arg194Trp) and X-ray repair cross complementary 3 (Thr241Met) polymorphism with the risk of breast cancer in females from northeastern region of India. The present case-control study includes histopathologically confirmed and newly diagnosed 464 cases with breast cancer and 534 apparently healthy neighborhood community controls. Information on sociodemographic factors and putative risk factors were collected from each study participant by conducting face-to-face interviews. Genotyping of X-ray repair cross complementary 1A (Arg194Trp) and X-ray repair cross complementary 3 (Thr241Met) was carried out by polymerase chain reaction-restriction fragment length polymorphism. For statistical analysis, both univariate and multivariate logistic regression analyses were performed. We also performed stratified analysis to find out the association of X-ray repair cross complementary genes with the risk of breast cancer stratified based on menstrual status. This study revealed that tryptophan allele (R/W-W/W genotype) in X-ray repair cross complementary 1A (Arg194Trp) gene significantly increased the risk of breast cancer (adjusted odds ratio = 1.44, 95% confidence interval = 1.06-1.97, P < .05 for R/W-W/W genotype). Moreover, it was found that tryptophan allele (W/W genotype) at codon 194 of X-ray repair cross complementary 1A (Arg194Trp) gene significantly increased the risk of breast cancer in premenopausal females (crude odds ratio = 1.66, 95% confidence interval = 1.11-2.46, P < .05 for R/W-W/W genotype). The present study did not reveal any significant association of X-ray repair cross complementary 3 (Thr241Met) polymorphism with the risk of breast cancer. The present study has explored that X-ray repair cross complementary 1A (Arg194Trp) gene polymorphism is significantly associated with the increased risk of breast cancer in premenopausal females from northeastern region of India which may be beneficial for prognostic purposes.

  12. DNA Repair Mechanism Gene, XRCC1A (Arg194Trp) but not XRCC3 (Thr241Met) Polymorphism Increased the Risk of Breast Cancer in Premenopausal Females: A Case–Control Study in Northeastern Region of India

    PubMed Central

    Ahmed, Jishan; Narain, Kanwar; Mukherjee, Kaustab; Majumdar, Gautam; Chenkual, Saia; Zonunmawia, Jason C.

    2017-01-01

    X-ray repair cross complementary group gene is one of the most studied candidate gene involved in different types of cancers. Studies have shown that X-ray repair cross complementary genes are significantly associated with increased risk of breast cancer in females. Moreover, studies have revealed that X-ray repair cross complementary gene polymorphism significantly varies between and within different ethnic groups globally. The present case–control study was aimed to investigate the association of X-ray repair cross complementary 1A (Arg194Trp) and X-ray repair cross complementary 3 (Thr241Met) polymorphism with the risk of breast cancer in females from northeastern region of India. The present case–control study includes histopathologically confirmed and newly diagnosed 464 cases with breast cancer and 534 apparently healthy neighborhood community controls. Information on sociodemographic factors and putative risk factors were collected from each study participant by conducting face-to-face interviews. Genotyping of X-ray repair cross complementary 1A (Arg194Trp) and X-ray repair cross complementary 3 (Thr241Met) was carried out by polymerase chain reaction-restriction fragment length polymorphism. For statistical analysis, both univariate and multivariate logistic regression analyses were performed. We also performed stratified analysis to find out the association of X-ray repair cross complementary genes with the risk of breast cancer stratified based on menstrual status. This study revealed that tryptophan allele (R/W-W/W genotype) in X-ray repair cross complementary 1A (Arg194Trp) gene significantly increased the risk of breast cancer (adjusted odds ratio = 1.44, 95% confidence interval = 1.06-1.97, P < .05 for R/W-W/W genotype). Moreover, it was found that tryptophan allele (W/W genotype) at codon 194 of X-ray repair cross complementary 1A (Arg194Trp) gene significantly increased the risk of breast cancer in premenopausal females (crude odds ratio = 1.66, 95% confidence interval = 1.11-2.46, P < .05 for R/W-W/W genotype). The present study did not reveal any significant association of X-ray repair cross complementary 3 (Thr241Met) polymorphism with the risk of breast cancer. The present study has explored that X-ray repair cross complementary 1A (Arg194Trp) gene polymorphism is significantly associated with the increased risk of breast cancer in premenopausal females from northeastern region of India which may be beneficial for prognostic purposes. PMID:29332455

  13. Pin1-FADD interactions regulate Fas-mediated apoptosis in activated eosinophils#

    PubMed Central

    Oh, Jiyoung; Malter, James S.

    2013-01-01

    Abnormally long-lived eosinophils (Eos) are the major inflammatory component of allergic responses in the lungs of active asthmatics. Eos recruited to the airways after allergen exposure produce and respond to IL-5 and GM-CSF, enhancing their survival. Pro-survival signaling activates Pin1, a cis-trans peptidyl isomerase (PPIase) that binds to Bax and prevents it activation. How long-lived Eos, despite the continued presence of GM-CSF or IL-5, eventually undergo apoptosis to end allergic inflammation remains unclear. Here we show that Pin1 location, activity and protein interactions are jointly influenced by Fas and pro-survival cytokine IL-5. Fas signaling strongly induced the phosphorylation of FADD at Ser194 and Pin1 at Ser16 as well as their nuclear accumulation. Phospho-mimic Ser194Glu FADD mutants accelerated Eos apoptosis compared to WT or Ser194Ala mutants. Downstream of FADD phosphorylation, Caspase 8, 9 and 3 cleavage as well as Eos apoptosis induced by Fas were reduced by constitutively active Pin1 and enhanced by Pin1 inhibition. Pin1 was activated by IL-5 while simultaneous IL-5 and anti-Fas treatment modestly reduced PPIase activity but induced Pin1 to associate with FADD after its phosphorylation at Ser194. Mechanistically, Pin1 mediated isomerization facilitated the subsequent dephosphorylation of Ser194 FADD and maintenance of cytoplasmic location. In vivo activated bronchoalvelolar (BAL) Eos obtained after allergen challenge showed elevated survival and Pin1 activity that could be reversed by anti-Fas. Therefore, our data suggest that Pin1 is a critical link between FADD mediated cell death and IL-5 mediated pro-survival signaling. PMID:23606538

  14. [Maple syrup urine disease caused by two novel BCKDHB gene mutations in a Chinese neonate].

    PubMed

    Shen, Yunlin; Gong, Xiaohui; Yan, Jingbin; Qin, Li; Qiu, Gang

    2015-01-01

    Maple syrup urine disease (MSUD) is an autosomal recessive metabolic disorder that is caused by mutations in the subunits of the branched chain α-ketoacid dehydrogenase (BCKD) complex. This report presents a Han ethnic Chinese newborn infant with the severe classic form of MSUD caused by two novel missense mutations in the BCKDHB gene. The clinical and biochemical data of a Chinese neonate with classic form of MSUD were analyzed, and the DNA sequences of BCKDHA, BCKDHB, DBT and DLD genes were investigated for mutations. Then the DNA samples of the proband and the patient's parents were tested with Sanger sequencing. The manifestations of this patient were poor feeding, low reaction, and compensatory metabolic acidosis. Tandem mass spectrometry (MS/MS) showed that leucine and valine were significantly higher than normal. Urine gas chromatography-mass spectrometry (GC/MS) showed significant abnormality. Brain CT scan showed white matter changes. We identified two previously unreported mutations in the BCKDHB gene, p.Leu194Phe (c.580 C>T) and p.Ser199Arg (c.597 T>G) in exon 5. Segregation analysis showed that the novel mutation p.Ser199Arg was maternally inherited and the novel mutation p.Leu194Phe was paternally inherited. Neither mutation was found in the 186 alleles of 93 normal Han ethnic Chinese individuals. In human BCKDHB protein crystal structure, the 194th and 199th amino acids changes are likely to affect the spatial structure of the protein. The 194th and 199th amino acid of human BCKDHB protein was conserved among species. PolyPhen protein function prediction indicated that the 194th and 199th amino acid changes were likely to affect protein function. Two novel missense mutations were identified in the BCKDHB gene in the Chinese patient with MSUD.

  15. A comparison of radiative transfer models for predicting the microwave emission from soils

    NASA Technical Reports Server (NTRS)

    Schmugge, T. J.; Choudhury, B. J.

    1980-01-01

    Two general types of numerical models for predicting microwave emission from soils are compared-coherent and noncoherent. In the former, radiation in the soil is treated coherently, and the boundary conditions on the electric fields across the layer boundaries are used to calculate the radiation intensity. In the latter, the radiation is assumed to be noncoherent, and the intensities of the radiation are considered directly. The results of the two approaches may be different because of the effects of interference, which can cause the transmitted intensity at the surface (i.e., emissivity) to be sometimes higher and sometimes lower for the coherent case than for the noncoherent case, depending on the relative phases of reflected fields from the lower layers. This coupling between soil layers in the coherent models leads to greater soil moisture sampling depths observed with this type of model, and is the major difference that is found between the two types of models. In noncoherent models, the emissivity is determined by the dielectric constraint at the air/soil interface. The subsequent differences in the results are functions of both the frequency of the radiation being considered and the steepness of the moisture gradient near the surface. The calculations were performed at frequencies of 1.4 and 19.4 GHz and for two sets of soil profiles. Little difference was observed between the models at 19.4 GHz; and only at the lower frequency were differences apparent because of the greater soil moisture sampling depth at this frequency.

  16. The correlation of Skylab L-band brightness temperatures with antecedent precipitation

    NASA Technical Reports Server (NTRS)

    Mcfarland, M. J.

    1975-01-01

    The S194 L-band radiometer flown on the Skylab mission measured terrestrial radiation at the microwave wavelength of 21.4 cm. The terrain emissivity at this wavelength is strongly dependent on the soil moisture content, which can be inferred from antecedent precipitation. For the Skylab data acquisition pass from the Oklahoma panhandle to southeastern Texas on 11 June 1973, the S194 brightness temperatures are highly correlated with antecedent precipitation from the preceding eleven day period, but very little correlation was apparent for the preceding five day period. The correlation coefficient between the averaged antecedent precipitation index values and the corresponding S194 brightness temperatures between 230 K and 270 K, the region of apparent response to soil moisture in the data, was -0.97. The equation of the linear least squares line is given.

  17. Environmentally Sustainable Yellow Smoke Formulations for Use in the M194 Hand Held Signal

    DTIC Science & Technology

    2012-06-10

    of the pyrotechnic train through the system hardware, potassium chlorate (oxidizer) and the sugar (fuel, or reducing agent) engage in a reduction...1: Composition of M194 control formulation Ingredient Wt. % Function Potassium Chlorate 35.0 Oxidizer Sugar (sucrose) 20.0 Fuel Vat yellow...Experimental Section 1. Materials Potassium chlorate , potassium nitrate, sodium bicarbonate, stearic acid, and sugar were purchased from Hummel

  18. Concentration and spatial distribution of lead in soil used for ammunition destruction.

    PubMed

    do Nascimento Guedes, Jair; do Amaral Sobrinho, Nelson Moura Brasil; Ceddia, Marcos Bacis; Vilella, André Luis Oliveira; Tolón-Becerra, Alfredo; Lastra-Bravo, Xavier Bolívar

    2012-10-01

    Studies on heavy metal contamination in soils used for ammunition disposal and destruction are still emerging. The present study aimed to evaluate the contamination level and spatial distribution of lead in disposal and destruction areas. This site was used for ammunition disposal and destruction activities for 20 years. The ammunition destruction site (1,296 ha), a sampling system that followed a sampling grid (5 m × 5 m) with 30 points was adopted and samples were collected at the following five depths with a total of 150 samples. During the collection procedure, each sampling grid point was georeferenced using a topographic global positioning system. Data were validated through semivariogram and kriging models using Geostat software. The results demonstrated that the average lead value was 163 mg kg(-1), which was close to the investigation limit and the contamination levels were higher downstream than upstream. The results showed that there was lead contamination at the destruction site and that the contamination existed mainly at the surface layer depth. However, high lead concentrations were also found at deeper soil depths in the destruction area due to frequent detonations. According to the planimetry data, the areas that require intervention significantly decreased with increasing depths in the following order: 582.7 m(2) in the 0-20 cm layer; 194.6 m(2) in the 20-40 cm layer; 101.6 m(2) in the 40-60 cm layer; and 45.3 m(2) in the 60-80 cm layer.

  19. XRCC1 Arg194Trp and Arg399Gln polymorphisms and arsenic methylation capacity are associated with urothelial carcinoma

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chiang, Chien-I; Huang, Ya-Li; Chen, Wei-Jen

    The association between DNA repair gene polymorphisms and bladder cancer has been widely studied. However, few studies have examined the correlation between urothelial carcinoma (UC) and arsenic or its metabolites. The aim of this study was to examine the association between polymorphisms of the DNA repair genes, XRCC1 Arg194Trp, XRCC1 Arg399Gln, XRCC3 Thr241Met, and XPD Lys751Gln, with urinary arsenic profiles and UC. To this end, we conducted a hospital-based case–control study with 324 UC patients and 647 age- and gender-matched non-cancer controls. Genomic DNA was used to examine the genotype of XRCC1 Arg194Trp, XRCC1 Arg399Gln, XRCC3 Thr241Met, and XPD Lys751Glnmore » by PCR-restriction fragment length polymorphism analysis (PCR-RFLP). Urinary arsenic profiles were measured by high performance liquid chromatography (HPLC) linked with hydride generator and atomic absorption spectrometry. The XRCC1 399 Gln/Gln and 194 Arg/Trp and Trp/Trp genotypes were significantly related to UC, and the odds ratio (OR) and 95% confidence interval (95%CI) were 1.68 (1.03–2.75) and 0.66 (0.48–0.90), respectively. Participants with higher total urinary arsenic levels, a higher percentage of inorganic arsenic (InAs%) and a lower percentage of dimethylarsinic acid (DMA%) had a higher OR of UC. Participants carrying XRCC1 risk diplotypes G-C/G-C, A-C/A-C, and A-T/G-T, and who had higher total arsenic levels, higher InAs%, or lower DMA% compared to those with other XRCC1 diplotypes had a higher OR of UC. Our results suggest that the XRCC1 399 Gln/Gln and 194 Arg/Arg DNA repair genes play an important role in poor arsenic methylation capacity, thereby increasing the risk of UC in non-obvious arsenic exposure areas. - Highlights: • The XRCC1 399Gln/Gln genotype was significantly associated with increased OR of UC. • The XRCC1 194 Arg/Trp and Trp/Trp genotype had a significantly decreased OR of UC. • Combined effect of the XRCC1 genotypes and poor arsenic methylation capacity on UC.« less

  20. The Surface Warfare Test Ship

    DTIC Science & Technology

    2000-01-26

    Dimensions: 194 x 228 in (including pedestal) "* Antenna weight: 5684 lbs (above deck), 24,018 (below deck) The aft mast (Figure 7-3) is modified to...d) The OJ- 194 console is laid-up. e) The bridge wing bulkheads are extended completely around the wings for RCS reduction. f) Two 30-person life...CE’s taps these monitored signals and transmit the data to the ITCS workstation. "* A control element activates HALON and AFFF bilge sprinkling

  1. Fisheries Handbook of Engineering Requirements and Biological Criteria

    DTIC Science & Technology

    1990-01-01

    of eggs dividual species. When trays are used for chum fry, bio- in incubators where IHNV is found. Additional space or rings or flexi-saddles are...recommended. limited loading of eggs and fry are both means of reducing the infection of IHNV . It has been found that hatchery fry at swim-up time are...effects, 13.5 IHNV , 193, 19.4 Kaplan turbines, 25.1 Mechanical handling, Ch. 33 labor-saving devices, 19.4 Kokanee ,landiocked sockeye) Mechanical hauling

  2. Sepsis in Children: Global Implications of the World Health Assembly Resolution on Sepsis.

    PubMed

    Kissoon, Niranjan; Reinhart, Konrad; Daniels, Ron; Machado, Machado Flavia R; Schachter, Raymond D; Finfer, Simon

    2017-12-01

    Sepsis, worldwide the leading cause of death in children, has now been recognized as the global health emergency it is. On May 26, 2017, the World Health Assembly, the decision-making body of the World Health Organization, adopted a resolution proposed by the Global Sepsis Alliance to improve the prevention, diagnosis, and management of sepsis. To discuss the implications of this resolution for children worldwide. The resolution highlights sepsis as a global threat and urges the 194 United Nations member states to take specific actions and implement appropriate measures to reduce its human and health economic burden. The resolution is a major step toward achieving the targets outlined by the Sustainable Developmental Goals for decreasing mortality in infants and children, but implementing it will require a concerted global effort.

  3. Positive impact of infection prevention on the management of nosocomial outbreaks at an academic hospital.

    PubMed

    Dik, Jan-Willem H; Sinha, Bhanu; Lokate, Mariëtte; Lo-Ten-Foe, Jerome R; Dinkelacker, Ariane G; Postma, Maarten J; Friedrich, Alexander W

    2016-10-01

    Infection prevention (IP) measures are vital to prevent (nosocomial) outbreaks. Financial evaluations of these are scarce. An incremental cost analysis for an academic IP unit was performed. On a yearly basis, we evaluated: IP measures; costs thereof; numbers of patients at risk for causing nosocomial outbreaks; predicted outbreak patients; and actual outbreak patients. IP costs rose on average yearly with €150,000; however, more IP actions were undertaken. Numbers of patients colonized with high-risk microorganisms increased. The trend of actual outbreak patients remained stable. Predicted prevented outbreak patients saved costs, leading to a positive return on investment of 1.94. This study shows that investments in IP can prevent outbreak cases, thereby saving enough money to earn back these investments.

  4. Mesoporous Akaganeite of Adjustable Pore Size Synthesized using Mixed Templates

    NASA Astrophysics Data System (ADS)

    Zhang, Y.; Ge, D. L.; Ren, H. P.; Fan, Y. J.; Wu, L. M.; Sun, Z. X.

    2017-12-01

    Mesoporous akaganeite with large and adjustable pore size was synthesized through a co-template method, which was achieved by the combined interaction between PEG2000 and alkyl amines with different lengths of the straight carbon chain. The characterized results indicate that the synthesized samples show comparatively narrow BJH pore size distributions and centered at 14.3 nm when PEG and HEPA was used, and it could be enlarged to 16.8 and 19.4 nm respectively through changing the alkyl amines to DDA and HDA. Meanwhile, all the synthesized akaganeite possess relativity high specific surface area ranging from 183 to 281 m2/g and high total pore volume of 0.98 to 1.5 cm3/g. A possible mechanism leading to the pore size changing was also proposed.

  5. Observation of two new L4 Neptune Trojans in the Dark Energy Survey supernova fields

    DOE PAGES

    Gerdes, D. W.

    2016-01-28

    We report the discovery of the eighth and ninth known Trojans in stable orbits around Neptune's leading Lagrange point, L4. The objects 2014 QO 441 and 2014 QP 441 were detected in data obtained during the 2013-14 and 2014-15 observing seasons by the Dark Energy Survey, using the Dark Energy Camera (DECam) on the 4-meter Blanco telescope at Cerro Tololo Inter- American Observatory. Both are in high-inclination orbits (18.8° and 19.4° respectively). Furthermore, with an eccentricity of 0.104, 2014 QO 441 has the most eccentric orbit of the eleven known stable Neptune Trojans. We describe the search procedure and investigatemore » the objects' long-term dynamical stability and physical properties.« less

  6. [Guidelines on medically assisted reproduction: legal issues and professional liability].

    PubMed

    Molinelli, A; Motroni Gherardi, S M; Picchioni, D M; Ventura, F

    2007-08-01

    The authors analyze the legal and medico-legal issues deriving from the recent Law No. 40 of February 19, 2004 concerning the Medically Assisted Reproduction. In particular, they analyze the contrasting points between the dispositions of Law No. 40/2004 and those of Law No. 194/1978 on the voluntary interruption of pregnancy, and they analyze the guidelines about the procedures and the techniques of the Medically Assisted Reproduction, issued by the Ministry of Health with D.M. of July 21, 2004. The Guidelines, as well as some sentences of several courts, lead to some reflections also about the consent and the professional liability, in particular considering the various moments of the medical action, from the first interview to the carrying out of the assisted reproduction techniques.

  7. The Value of the Electrocardiogram for Evaluating Prognosis in Patients with Idiopathic Pulmonary Arterial Hypertension.

    PubMed

    Cheng, Xiao-Ling; He, Jian-Guo; Liu, Zhi-Hong; Gu, Qing; Ni, Xin-Hai; Zhao, Zhi-Hui; Luo, Qin; Xiong, Chang-Ming

    2017-02-01

    Association between electrocardiography (ECG) features and right ventricular anatomy and physiology has been established. This study is aimed to identify the value of 12-lead ECG in evaluating prognosis of patients with idiopathic pulmonary arterial hypertension (IPAH). 194 patients with newly diagnosed IPAH were included in this study. Correlations between electrocardiography variables and hemodynamics were assessed. Univariate and multivariable cox regression analysis were performed to identify ECG variables for predicting all-cause mortality in IPAH. Partial correlation analysis showed that P wave amplitude in lead II correlated with the mean pulmonary arterial pressure (mPAP, r = 0.349, p ≤ 0.001) and cardiac index (CI, r = -0.224, p = 0.002); R wave amplitude in V1 correlated with mPAP (r = 0.359, p ≤ 0.001); S wave amplitude in V6 correlated with mPAP (r = 0.259, p = 0.030) and CI (r = -0.220, p = 0.003). P wave amplitude in lead II (HR 1.555, p = 0.033) and R wave amplitude in lead aVR (HR 5.058, p < 0.001) were the independent predictors of all-cause mortality. Kaplan-Meier survival curves showed patients with a p ≥ 0.25 mv in lead II, and R ≥ 0.4 mv in lead aVR had lower 3-year survival (55 vs. 91%, p < 0.001). Specific lead-12 ECG features could reflect right ventricular overload hemodynamics, and are useful to evaluate prognosis of patients with IPAH.

  8. Adjacent segment infection after surgical treatment of spondylodiscitis.

    PubMed

    Siam, Ahmed Ezzat; El Saghir, Hesham; Boehm, Heinrich

    2016-03-01

    This is the first case series to describe adjacent segment infection (ASI) after surgical treatment of spondylodiscitis (SD). Patients with SD, spondylitis who were surgically treated between 1994 and 2012 were included. Out of 1187 cases, 23 (1.94 %) returned to our institution (Zentralklinik Bad Berka) with ASI: 10 males, 13 females, with a mean age of 65.1 years and a mean follow-up of 69 months. ASI most commonly involved L3-4 (seven patients), T12-L1 (five) and L2-3 (four). The mean interval between operations of primary infection and ASI was 36.9 months. All cases needed surgical intervention, debridement, reconstruction and fusion with longer instrumentation, with culture and sensitivity-based postoperative antimicrobial therapy. At last follow-up, six patients (26.1 %) were mobilized in a wheelchair with a varying degree of paraplegia (three had pre-existing paralysis). Three patients died within 2 months after the ASI operation (13 %). Excellent outcomes were achieved in five patients, and good in eight. Adjacent segment infection after surgical treatment of spondylodiscitis is a rare complication (1.94 %). It is associated with multimorbidity and shows a high mortality rate and a high neurological affection rate. Possible explanations are: haematomas of repeated micro-fractures around screw loosening, haematogenous spread, direct inoculation or a combination of these factors. ASI may also lead to proximal junctional kyphosis, as found in this series. We suggest early surgical intervention with anterior debridement, reconstruction and fusion with posterior instrumentation, followed by antimicrobial therapy for 12 weeks. Level IV retrospective uncontrolled case series.

  9. Chemical and biochemical composition of caviar from different sturgeon species and origins.

    PubMed

    Wirth, M; Kirschbaum, F; Gessner, J; Krüger, A; Patriche, N; Billard, R

    2000-08-01

    The chemical and biochemical composition of caviar in 22 specimens of wild caught and of 2 farmed animals were measured. The results include grain size, protein and fat content, fatty acid composition of triglycerides and phospholipids, as well as the concentrations of relevant heavy metals and chlorinated hydrocarbons. The average protein content varied between 26.2 and 31.1% (wet weight) and fat from 10.9 to 19.4% (wet weight) with lowest values for caviar from farmed sturgeon. The triglycerides and phospholipids contained more n-3 fatty acids, especially eicosapentaenoic and docosahexaenoic acid than n-6 fatty acids. The copper and zinc concentrations varied between 1.20 and 1.69 and 10.3 and 12.4 mg/kg (wet weight), respectively. These values reflect the elevated requirement of sturgeons for these components. Lead content varied between 0.06 and 0.15 mg/kg (wet weight). The cadmium concentrations were less than 5 micrograms/kg (wet weight) leading to the conclusion that no accumulation took place in the eggs. The concentrations of sigma DDT and sigma PCB were extremely high in caviar from Huso huso compared to the samples of the other species thus reflecting the different food habits leading to increased bio-accumulation.

  10. VizieR Online Data Catalog: Herschel SPIRE/FTS 194-671um survey of GOALS LIRGs (Lu+, 2017)

    NASA Astrophysics Data System (ADS)

    Lu, N.; Zhao, Y.; Diaz-Santos, T.; Xu, C. K.; Gao, Y.; Armus, L.; Isaak, K. G.; Mazzarella, J. M.; van der Werf, P. P.; Appleton, P. N.; Charmandaris, V.; Evans, A. S.; Howell, J.; Iwasawa, K.; Leech, J.; Lord, S.; Petric, A. O.; Privon, G. C.; Sanders, D. B.; Schulz, B.; Surace, J. A.

    2017-06-01

    In this paper we presented a Herschel SPIRE/FTS 194-671um spectroscopic survey of 121 galaxies belonging to a complete, flux-limited sample of 123 luminous infrared galaxies (LIRGs) down to a total IR flux of 6.5x10-13W/m2, selected from the Great Observatories All-Sky LIRG Survey (GOALS; Armus+ 2009PASP..121..559A). All 123 observed targets are listed in Table 1. (3 data files).

  11. SU-F-I-56: High-Precision Gamma-Ray Analysis of Medical Isotopes

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chopra, N; Chillery, T; Chowdhury, P

    2016-06-15

    Purpose: Advanced, time-resolved, Compton-suppressed gamma-ray spectroscopy with germanium detectors is implemented for assaying medical isotopes to study the radioactive decay process leading to a more accurate appraisal of the received dose and treatment planning. Lowell’s Array for Radiological Assay (LARA), a detector array that is comprised of six Compton-suppressed high-purity germanium detectors, is currently under development at UMass-Lowell which combines Compton-suppression and time-and-angle correlations to allow for highly efficient and highly sensitive measurements. Methods: Two isotopes produced Brookhaven Linac Isotope Producer (BLIP) were investigated. {sup 82}Sr which is the parent isotope for producing {sup 82}Rb is often used in cardiacmore » PET. {sup 82}Sr gamma-ray spectrum is dominated by the 511keV photons from positron annihilation which prevent precise measurement of co-produced contaminant isotopes. A second project was to investigate the production of platinum isotopes. Natural platinum was bombarded with protons from 53MeV to 200MeV. The resulting spectrum was complicated due to the large number of stable platinum isotopes in the target, the variety of open reaction channels (p,xn), (p,pxn), (p,axn). Results: By using face-to-face NaI(Tl) counters 90-degrees to the Compton-suppressed germaniums to detect the 511keV photons, a much cleaner and more sensitive measurement of {sup 85}Sr and other contaminants was obtained. For the platinum target, we identified the production of {sup 188–189–191–195}Pt, {sup 191–192–193–194–195–196}Au and {sup 186–188–189–190–192–194–189–190–192–194}Ir. For example, at the lower energies (53 and 65MeV), we measured {sup 191}Pt production cross-sections of 144mb and 157mb. Considerable care was needed in following the process of dissolving and diluting the samples to get consistent results. The new LARA array will help us better ascertain the absolute efficiency of the counting system and more reliable production cross-sections. Conclusion: Modern HPGe spectroscopic techniques provide enhanced sensitivity, promising precise quantification of the quality of radioisotopes used in medical physics. Using new decay information may have non-trivial impact on treatment planning and dose-assessment.« less

  12. A comprehensive evaluation of the efficacy of leading oxime therapies in guinea pigs exposed to organophosphorus chemical warfare agents or pesticides

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wilhelm, Christina M., E-mail: wilhelmc@battelle.org; Snider, Thomas H., E-mail: snidert@battelle.org; Babin, Michael C., E-mail: babinm@battelle.org

    The currently fielded pre-hospital therapeutic regimen for the treatment of organophosphorus (OP) poisoning in the United States (U.S.) is the administration of atropine in combination with an oxime antidote (2-PAM Cl) to reactivate inhibited acetylcholinesterase (AChE). Depending on clinical symptoms, an anticonvulsant, e.g., diazepam, may also be administered. Unfortunately, 2-PAM Cl does not offer sufficient protection across the range of OP threat agents, and there is some question as to whether it is the most effective oxime compound available. The objective of the present study is to identify an oxime antidote, under standardized and comparable conditions, that offers protection atmore » the FDA approved human equivalent dose (HED) of 2-PAM Cl against tabun (GA), sarin (GB), soman (GD), cyclosarin (GF), and VX, and the pesticides paraoxon, chlorpyrifos oxon, and phorate oxon. Male Hartley guinea pigs were subcutaneously challenged with a lethal level of OP and treated at approximately 1 min post challenge with atropine followed by equimolar oxime therapy (2-PAM Cl, HI-6 DMS, obidoxime Cl{sub 2}, TMB-4, MMB4-DMS, HLö-7 DMS, MINA, and RS194B) or therapeutic-index (TI) level therapy (HI-6 DMS, MMB4-DMS, MINA, and RS194B). Clinical signs of toxicity were observed for 24 h post challenge and blood cholinesterase [AChE and butyrylcholinesterase (BChE)] activity was analyzed utilizing a modified Ellman's method. When the oxime is standardized against the HED of 2-PAM Cl for guinea pigs, the evidence from clinical observations, lethality, quality of life (QOL) scores, and cholinesterase reactivation rates across all OPs indicated that MMB4 DMS and HLö-7 DMS were the two most consistently efficacious oximes. - Highlights: • First comprehensive evaluation of leading AChE oxime reactivators • All oximes are compared against current U.S. therapy 2-PAM Cl. • Relative therapeutic oxime efficacies against OP CWNA and pesticides • Contribution to more effective antidotes for civilian and military populations.« less

  13. Control of key pecan insect pests using biorational pesticides.

    PubMed

    Shapiro-Ilan, David I; Cottrell, Ted E; Jackson, Mark A; Wood, Bruce W

    2013-02-01

    Key pecan insect pests include the black pecan aphid, Melanocallis caryaefoliae (Davis), pecan weevil, Curculio caryae (Horn), and stink bugs (Hemiptera: Pentatomidae). Alternative control tactics are needed for management of these pests in organic and conventional systems. Our objective was to evaluate the potential utility of several alternative insecticides including three plant extract formulations, eucalyptus extract, citrus extract-8.92%, and citrus extract-19.4%, and two microbial insecticides, Chromobacterium subtsugae (Martin et al.) and Isaria fumosorosea (Wize). In the laboratory, eucalyptus extract, citrus extract-8.92%, citrus extract-19.4%, and C. subtsugae caused M. caryaefoliae mortality (mortality was reached approximately 78, 83, and 96%, respectively). In field tests, combined applications of I. fumosorosea with eucalyptus extract were synergistic and caused up to 82% mortality in M. caryaefoliae. In laboratory assays focusing on C. caryae suppression, C. subtsugae reduced feeding and oviposition damage, eucalyptus extract and citrus extract-19.4% were ineffective, and antagonism was observed when citrus extract-19.4% was combined with the entomopathogenic nematode, Steinernema carpocapsae (Weiser). In field tests, C. subtsugae reduced C. caryae damage by 55% within the first 3d, and caused 74.5% corrected mortality within 7 d posttreatment. In the laboratory, C. subtsugae and eucalyptus extract did not cause mortality in the brown stink bug, Euschistus servus (Say). Applications of C. subtsugae for suppression of C. caryae, and eucalyptus extract plus I. fumosorosea for control of M. caryaefoliae show promise as alternative insecticides and should be evaluated further.

  14. [Presence of islands of fatty tissue in struma].

    PubMed

    Lax, S; Beham, A; Langsteger, W; Schmid, C

    1988-04-01

    We examined 929 surgically obtained thyroid glands derived from 791 female and 138 male patients and found intrathyroidal adipose tissue in 18 cases (1.94%). Only the thyroid glands of middle aged and older women (mean age 51 years) were affected, as well as in the case of follicular adenomas and nodular and diffuse hyperplasia. With respect to localization in the interstitial connective tissue, as well as between thyroid follicles, interstitial, parenchymatous and mixed types are proposed. In addition to sex and age there is a high correlation with obesity, which is suggestive of hormonal influences. Knowledge of the presence of intrathyroidal fat tissue is of interest to the pathologist since it may lead to erroneous interpretations of histological and cytological specimens of the thyroid gland. In conclusion, not one case of hypothyroidism was detected amongst the 18 cases investigated in this study.

  15. MITLL 2015 Language Recognition Evaluation System Description

    DTIC Science & Technology

    2016-01-27

    912 8.18 qsl-rus Russian 2021 37.80 ara-ary Maghrebi 919 46.91 spa-car Carib. Spa. 194 30.59 ara-arz Egyptian 440 97.27 spa-eur Eur. Spa. 366 8.55...qsl-pol Polish 695 32.14 ara-arb MSA 912 8.18 qsl-rus Russian 2021 37.80 ara-ary Maghrebi 919 46.91 spa-car Carib. Spa. 194 30.59 ara-arz Egyptian ...BOTTLENECK I-VECTOR SYSTEM (BNF1) The Deep Neural Network architecture that we used for this system was composed of seven hidden layers. The sixth

  16. Introduction of a new laser-scalpel for partial kidney resection based on 1.94 micrometer fiber laser system: initial in vivo-data

    NASA Astrophysics Data System (ADS)

    Tedsen, Sönke; Theisen-Kunde, Dirk; Doehn, Christian; Kausch, Ingo; Jocham, Dieter

    2009-02-01

    The technique of nephron sparing surgery has matured significantly over the past decade and is emerging as an oncologically sound procedure for the management of renal tumors. Methods of tumor excision as well as parenchymal reconstruction in a hemostaticallly controlled field have evolved to make this procedure safer. In an attempt to find an improoved hemostatic cutting instrument we developed a 1.94 micrometer Laser-Scalpel system in a porcine model. We evaluated data for partial porcine kidney resection performed by a 1.94 micrometer Laser-Scalpel and compared the data to those of a standard HF- (High- Frequency) dissection device. In 12 pigs general anesthesia and a median laparotomy was performed to expose both kidneys. In each pig one kidney was partially resected with the Laser-Scalpel and the other side with the HF-dissection device. The first 6 pigs were euthanized immediately after the procedure. The following 6 pigs were allowed to recover and underwent 2-3 weeks later euthanasia. The final evaluation data included total resection time, blood loss, mass of dissected tissue, total ischemic time and histological examination. Mean resected kidney tissue mass was 4.75 g with the laser system and 5.57 g for the HF-dissector, respectively. Mean estimated blood loss was 22 ml for the Laser- Scalpel and 78.2 ml for the HF-dissection device. Resection time was 9.45 min for the Laser-scalpel compared to 10.16 min. No complications, specifically no postoperative bleeding, occured in any of the animals. Histological evaluation with H&E staining showed a carbonized zone of about 0.57 mm directly at the dissected edge followed by a thermal damaged zone of about 1.25 mm in width. Thereafter healthy tissue was found in all histological samples. Partial kidney resection was easily and fast performed by the use of a 1.94 micrometer Laser-Scalpel system. Hemostasis was highly sufficient, so blood loss was minimal compared to conventional HF-dissection device. Therefore the 1.94 micrometer Laser-Scalpel system is a very promising dissection device for urological surgery.

  17. Quaternary and tertiary aldoxime antidotes for organophosphate exposure in a zebrafish model system

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Schmidt, Hayden R.; Radić, Zoran; Taylor, Palmer

    The zebrafish is rapidly becoming an important model system for screening of new therapeutics. Here we evaluated the zebrafish as a potential pharmacological model for screening novel oxime antidotes to organophosphate (OP)-inhibited acetylcholinesterase (AChE). The k{sub i} values determined for chlorpyrifos oxon (CPO) and dichlorvos (DDVP) showed that CPO was a more potent inhibitor of both human and zebrafish AChE, but overall zebrafish AChE was less sensitive to OP inhibition. In contrast, aldoxime antidotes, the quaternary ammonium 2-PAM and tertiary amine RS-194B, showed generally similar overall reactivation kinetics, k{sub r}, in both zebrafish and human AChE. However, differences between themore » K{sub ox} and k{sub 2} constants suggest that zebrafish AChE associates more tightly with oximes, but has a slower maximal reactivation rate than human AChE. Homology modeling suggests that these kinetic differences result from divergences in the amino acids lining the entrance to the active site gorge. Although 2-PAM had the more favorable in vitro reactivation kinetics, RS-194B was more effective antidote in vivo. In intact zebrafish embryos, antidotal treatment with RS-194B rescued embryos from OP toxicity, whereas 2-PAM had no effect. Dechorionation of the embryos prior to antidotal treatment allowed both 2-PAM and RS-194B to rescue zebrafish embryos from OP toxicity. Interestingly, RS-194B and 2-PAM alone increased cholinergic motor activity in dechorionated embryos possibly due to the reversible inhibition kinetics, K{sub i} and αK{sub i}, of the oximes. Together these results demonstrate that the zebrafish at various developmental stages provides an excellent model for investigating membrane penetrant antidotes to OP exposure. - Highlights: • Zebrafish AChE shares significant structural similarities with human AChE. • OP-inhibited zebrafish and human AChE exhibit similar reactivation kinetics. • The zebrafish chorion is permeable to BBB penetrant and not charged aldoximes. • Zebrafish are a good aquatic model for studying centrally acting antidotes.« less

  18. Chandra Observation of the Cluster of Galaxies MS 0839.9+2938 at z = 0.194: The Central Excess Iron and Type Ia Supernova Enrichment

    NASA Astrophysics Data System (ADS)

    Wang, Yu; Xu, Haiguang; Zhang, Zhongli; Xu, Yueheng; Wu, Xiang-Ping; Xue, Sui-Jian; Li, Zongwei

    2005-09-01

    We present the Chandra study of the intermediate-redshift (z=0.194) cluster of galaxies MS 0839.9+2938. By performing both projected and deprojected spectral analyses, we find that the gas temperature is approximately constant at about 4 keV within 130-444 h-170 kpc. In the inner regions, the gas temperature decreases toward the center, reaching <~3 keV in the central 37 h-170 kpc. This implies that the lower and upper limits of the mass deposit rate are 9-34 and 96-126 Msolar yr-1, respectively, within 74 h-170 kpc, where the gas is significantly colder. Along with the temperature drop, we detect a significant inward iron abundance increase from about 0.4 Zsolar in the outer regions to ~=1 Zsolar within the central 37 h-170 kpc. Thus, MS 0839.9+2938 is the cluster showing the most significant central iron excess at z>~0.2. We argue that most of the excess iron should have been contributed by SNe Ia. Using the observed SN Ia rate and stellar mass loss rate, we estimate that the time needed to enrich the central region with excess iron is 6.4-7.9 Gyr, which is similar to those found for nearby clusters. Coinciding with the optical extension of the cD galaxy (up to about 30 h-170 kpc), the observed X-ray surface brightness profile exhibits an excess beyond the distribution expected by either the β model or the Navarro-Frenk-White (NFW) model and can be well fitted with an empirical two-β model that leads to a relatively flatter mass profile in the innermost region.

  19. A Comprehensive Evaluation of the Efficacy of Leading Oxime Therapies in Guinea Pigs Exposed to Organophosphorus Chemical Warfare Agents or Pesticides

    PubMed Central

    Wilhelm, Christina M.; Snider, Thomas H.; Babin, Michael C.; Jett, David A.; Platoff, Gennady E.; Yeung, David T.

    2014-01-01

    The currently fielded pre-hospital therapeutic regimen for the treatment of organophosphorus (OP) poisoning in the United States (U.S.) is the administration of atropine in combination with an oxime antidote (2-PAM Cl) to reactivate inhibited acetylcholinesterase (AChE). Depending on clinical symptoms, an anticonvulsant, e.g., diazepam, may also be administered. Unfortunately, 2-PAM Cl does not offer sufficient protection across the range of OP threat agents, and there is some question as to whether it is the most effective oxime compound available. The objective of the present study is to identify an oxime antidote, under standardized and comparable conditions, that offers protection at the FDA approved human equivalent dose (HED) of 2-PAM Cl against tabun (GA), sarin (GB), soman (GD), cyclosarin (GF), and VX, and the pesticides paraoxon, chlorpyrifos oxon, and phorate oxon. Male Hartley guinea pigs were subcutaneously challenged with a lethal level of OP and treated at approximately 1 min post challenge with atropine followed by equimolar oxime therapy (2-PAM Cl, HI-6 DMS, obidoxime Cl2, TMB-4, MMB4-DMS, HLö-7 DMS, MINA, and RS194B) or therapeutic-index (TI) level therapy (HI-6 DMS, MMB4-DMS, MINA, and RS194B). Clinical signs of toxicity were observed for 24 hours post challenge and blood cholinesterase [AChE and butyrylcholinesterase (BChE)] activity was analyzed utilizing a modified Ellman’s method. When the oxime is standardized against the HED of 2-PAM Cl for guinea pigs, the evidence from clinical observations, lethality, quality of life (QOL) scores, and cholinesterase reactivation rates across all OPs indicated that MMB4 DMS and HLö-7 DMS were the two most consistently efficacious oximes. PMID:25448441

  20. Determining the saliency of feature measurements obtained from images of sedimentary organic matter for use in its classification

    NASA Astrophysics Data System (ADS)

    Weller, Andrew F.; Harris, Anthony J.; Ware, J. Andrew; Jarvis, Paul S.

    2006-11-01

    The classification of sedimentary organic matter (OM) images can be improved by determining the saliency of image analysis (IA) features measured from them. Knowing the saliency of IA feature measurements means that only the most significant discriminating features need be used in the classification process. This is an important consideration for classification techniques such as artificial neural networks (ANNs), where too many features can lead to the 'curse of dimensionality'. The classification scheme adopted in this work is a hybrid of morphologically and texturally descriptive features from previous manual classification schemes. Some of these descriptive features are assigned to IA features, along with several others built into the IA software (Halcon) to ensure that a valid cross-section is available. After an image is captured and segmented, a total of 194 features are measured for each particle. To reduce this number to a more manageable magnitude, the SPSS AnswerTree Exhaustive CHAID (χ 2 automatic interaction detector) classification tree algorithm is used to establish each measurement's saliency as a classification discriminator. In the case of continuous data as used here, the F-test is used as opposed to the published algorithm. The F-test checks various statistical hypotheses about the variance of groups of IA feature measurements obtained from the particles to be classified. The aim is to reduce the number of features required to perform the classification without reducing its accuracy. In the best-case scenario, 194 inputs are reduced to 8, with a subsequent multi-layer back-propagation ANN recognition rate of 98.65%. This paper demonstrates the ability of the algorithm to reduce noise, help overcome the curse of dimensionality, and facilitate an understanding of the saliency of IA features as discriminators for sedimentary OM classification.

  1. Identification and Quantification of Gingerols and Related Compounds in Ginger Dietary Supplements Using High Performance Liquid Chromatography-Tandem Mass Spectrometry

    PubMed Central

    TAO, YI; LI, WENKUI; LIANG, WENZHONG; VAN BREEMEN, RICHARD B.

    2009-01-01

    Dietary supplements containing preparations of ginger roots/rhizomes (Zingiber officinale Roscoe) are being used by consumers, and clinical trials using ginger dietary supplements have been carried out to evaluate their anti-inflammatory or anti-emetic properties with inconsistent results. Chemical standardization of these products is needed for quality control and to facilitate the design of clinical trials and the evaluation of data from these studies. To address this issue, methods based on liquid chromatography-tandem mass spectrometry (LC-MS-MS) were developed for the detection, characterization and quantitative analysis of gingerol-related compounds in botanical dietary supplements containing ginger roots/rhizomes. During negative ion electrospray with collision induced-dissociation, the cleavage of the C4-C5 bond with a neutral loss of 194 u and benzylic cleavage leading to the neutral loss of 136 u were found to be class characteristic fragmentation patterns of the pharmacologically active gingerols or shogaols, respectively. Based on these results, an assay using LC-MS-MS with neutral loss scanning (loss of 194 u or 136 u) was developed that is suitable for the fingerprinting of ginger dietary supplements based on the selective detection of gingerols, shogaols, paradols, and gingerdiones. In addition, a quantitative assay based on LC-MS-MS with selected reaction monitoring was developed for the quantitative analysis of 6-gingerol, 8-gingerol, 10-gingerol, 6-shogaol, 8-shogaol, and 10-shogaol in ginger dietary supplements. After method validation, the quantities of these compounds in three commercially available ginger dietary supplements were determined. This assay showed excellent sensitivity, accuracy and precision and may be used to address the need for quality control and standardization of ginger dietary supplements. PMID:19817455

  2. Nicotinic Acid Adenine Dinucleotide Phosphate (NAADP)-mediated Calcium Signaling and Arrhythmias in the Heart Evoked by β-Adrenergic Stimulation*♦

    PubMed Central

    Nebel, Merle; Schwoerer, Alexander P.; Warszta, Dominik; Siebrands, Cornelia C.; Limbrock, Ann-Christin; Swarbrick, Joanna M.; Fliegert, Ralf; Weber, Karin; Bruhn, Sören; Hohenegger, Martin; Geisler, Anne; Herich, Lena; Schlegel, Susan; Carrier, Lucie; Eschenhagen, Thomas; Potter, Barry V. L.; Ehmke, Heimo; Guse, Andreas H.

    2013-01-01

    Nicotinic acid adenine dinucleotide phosphate (NAADP) is the most potent Ca2+-releasing second messenger known to date. Here, we report a new role for NAADP in arrhythmogenic Ca2+ release in cardiac myocytes evoked by β-adrenergic stimulation. Infusion of NAADP into intact cardiac myocytes induced global Ca2+ signals sensitive to inhibitors of both acidic Ca2+ stores and ryanodine receptors and to NAADP antagonist BZ194. Furthermore, in electrically paced cardiac myocytes BZ194 blocked spontaneous diastolic Ca2+ transients caused by high concentrations of the β-adrenergic agonist isoproterenol. Ca2+ transients were recorded both as increases of the free cytosolic Ca2+ concentration and as decreases of the sarcoplasmic luminal Ca2+ concentration. Importantly, NAADP antagonist BZ194 largely ameliorated isoproterenol-induced arrhythmias in awake mice. We provide strong evidence that NAADP-mediated modulation of couplon activity plays a role for triggering spontaneous diastolic Ca2+ transients in isolated cardiac myocytes and arrhythmias in the intact animal. Thus, NAADP signaling appears an attractive novel target for antiarrhythmic therapy. PMID:23564460

  3. Synthesis of Diketopiperizine Peptide Derivatives by Cross-Metathesis

    DTIC Science & Technology

    2006-05-01

    3079, 2914, 1656, 1487, 1441, 1415, 1336, 1294, 1193, 1142, 1074, 1011 cm’ ; HRMS (EI pos) for CjoH14N20 2 [M]+: calcd 194.1055, found 194.1047. Anal...3088, 2971, 1705, 1509, 1455, 1368, 1169, 1053; HRMS (CI pos) for C 17 H 2 4NO4 [M+H]+: calcd 306.1705, found 306.1703. Anal. calcd 12 for C 17H 23NO4...28.47, 18.81; IR (neat) 3368, 2980, 2937, 1716, 1650, 1518, 1455, 1367, 1251, 1167, 1069; HRMS (ESI-FTICR) for [M+Na]+, calcd 252.1206, found 252.1227

  4. Performance Oriented Packaging Testing of Wirebound Wood Box Containing Two M2A1 Ammunition Boxes for Packing Group II Hazardous Materials

    DTIC Science & Technology

    1992-01-01

    00-892-4302 FUZE M557 8861213 1.1B 0106 16 19.4 N335 1390-01-080-4171 FUZE M557 8861213 1.4B 0257 16 19.4 N340 1390-00-574-7705 FUZE M739 8861213...1.1D 0408 16 20.9 N340-1390-01-080-9447 FUZE M739 8861213 1.2D 0409 16 20.9 N340 1390-01-132-7481 FUZE M739A1 8861213 1.2D 0409 16 20.9 N402 1390-00-764

  5. Dollar Summary of Prime Contract Awards by Contractor, State or Country, and Place, FY83, Part 2 (Gillespie Delorenzo Asla & Assocs-Planning Analysis Corp).

    DTIC Science & Technology

    1983-01-01

    BUILDERS INC WRIGHT PATTERSON AFS OHIO 202 202 263 202 a LOCKHART WILLIAM M CORP SAN DIEGO CALIFORNIA 125 125 125 125 LOCKHEED AIRCRAFT SERVICE CO ONTARIO...THOUSANDS) CONTRACTOR CITY STATE AGGREGATE ARMY NAVY AIR FORCE DLA OCE LORENZ a WILLIAMS INC WRIGHT PATTERSON AFB OHIO 021 821 821 821 LORENZ BEN ASSOCIATES...MONMOUTH NEW JERSEY 32 32 GIULIANI CONTRACTING CO MCGUIRE AFB NEW JERSEY 158 158 258 100 158 GIVENS ULTIMATE SERVICES INC WRIGHT PATTERSON AFB OHIO 194 194

  6. Alternatives to argon for gas stopping volumes in the B194 neutron imager

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bleuel, D. L.; Anderson, S.; Caggiano, J. A.

    2017-05-17

    In a recent experiment at Lawrence Berkeley National Laboratory, the 40Ar(d,p)41Ar excitation function between 3-7 MeV was measured, confirming a previous estimation that there may be an intolerable radiation dose from 41Ar production by slowing to rest 6.74 MeV deuterons in the gas cell of the neutron imaging facility being constructed in B194. Gas alternatives to argon are considered, including helium, nitrogen (N2), neon, sulfur hexafluoride (SF6), krypton, and xenon, as well as high atomic number solid backings such as tantalum.

  7. Detection of Zika virus infection among asymptomatic pregnant women in the North of Peru.

    PubMed

    Weilg, Claudia; Troyes, Lucinda; Villegas, Zoila; Silva-Caso, Wilmer; Mazulis, Fernando; Febres, Ammy; Troyes, Mario; Aguilar-Luis, Miguel Angel; Del Valle-Mendoza, Juana

    2018-05-18

    To report an outbreak of ZIKV infection among asymptomatic pregnant women during 2016 in the city of Jaen, Cajamarca. Zika virus RNA was detected in 3.2% (n = 36) of cases by RT-PCR. The mean age of patients positive for ZIKV infection was 29.6 years. 7 patients (19.4%) infected with ZIKV were in their first-trimester of gestation, 13 (36.1%) were in their second-trimester, and 16 (44%) were in their third-trimester. All of the infected pregnant women were asymptomatic. ZIKV infection remains a major public health issue that calls for constant epidemiological surveillance. It can cause the congenital Zika virus syndrome in the newborns of infected mothers. The lack of molecular diagnostic methods in isolated localities and the similarity of symptoms to other arboviral infections, lead to an under-diagnosis of this disease in endemic areas.

  8. Youth-Adult Partnerships and Youth Identity Style.

    PubMed

    Ramey, Heather L; Rose-Krasnor, Linda; Lawford, Heather L

    2017-02-01

    Youth-adult partnerships (e.g., youth leading programs, participating as members of advisory boards) are a common and widely recommended practice in youth work and youth-serving program settings. Although researchers have suggested that these opportunities contribute to youth's identity development, empirical evidence is lacking. In the current study, we tested associations between identity style and degree of youth voice, collaborative youth-adult relationships, and youth's program engagement in 194 youth participating in youth-adult partnerships (M age  = 17.6, 62 % female). We found that these characteristics of youth-adult partnerships predicted higher informational identity style, although only program engagement emerged as a unique predictor. Furthermore, exploratory analysis indicated that these associations were moderated by the type of organization. The findings suggest the need for more research on the multiple dimensions of youth-adult partnerships and their association with youth functioning, as well as pointing to the importance of the broader organizational context of youth-adult partnerships.

  9. Stability and activity modulation of chymotrypsins in AOT reversed micelles by protein-interface interaction: interaction of alpha-chymotrypsin with a negative interface leads to a cooperative breakage of a salt bridge that keeps the catalytic active conformation (Ile16-Asp194).

    PubMed

    Almeida, F C; Valente, A P; Chaimovich, H

    1998-08-05

    The stability of alpha-chymotrypsin and delta-chymotrypsin was studied in reversed micelles of sodium bis(2-ethylhexyl)sulfosuccinate (AOT) in isooctane. alpha-Chymotrypsin is inactivated at the interface and at the water pool, while delta-chymotrypsin is inactivated only at the water pool. The mechanism of inactivation at the interface is related to the interaction of N-terminal group alanine 149 (absent in delta-chymotrypsin) with the negative interface. The dependence of enzyme activity on water content of these two enzymes in reversed micelles of AOT is also related with the interface interaction, since delta-chymotrypsin does not have a bell-shaped curve as observed for alpha-chymotrypsin. Copyright 1998 John Wiley & Sons, Inc.

  10. Multi-stage phononic crystal structure for anchor-loss reduction of thin-film piezoelectric-on-silicon microelectromechanical-system resonator

    NASA Astrophysics Data System (ADS)

    Bao, Fei-Hong; Bao, Lei-Lei; Li, Xin-Yi; Ammar Khan, Muhammad; Wu, Hua-Ye; Qin, Feng; Zhang, Ting; Zhang, Yi; Bao, Jing-Fu; Zhang, Xiao-Sheng

    2018-06-01

    Thin-film piezoelectric-on-silicon acoustic wave resonators are promising for the development of system-on-chip integrated circuits with micro/nano-engineered timing reference. However, in order to realize their large potentials, a further enhancement of the quality factor (Q) is required. In this study, a novel approach, based on a multi-stage phononic crystal (PnC) structure, was proposed to achieve an ultra-high Q. A systematical study revealed that the multi-stage PnC structure formed a frequency-selective band-gap to effectively prohibit the dissipation of acoustic waves through tethers, which significantly reduced the anchor loss, leading to an insertion-loss reduction and enhancement of Q. The maximum unloaded Q u of the fabricated resonators reached the value of ∼10,000 at 109.85 MHz, indicating an enhancement by 19.4 times.

  11. Seroprevalence of toxoplasmosis, toxocariasis and cysticercosis in a rural settlement, São Paulo State, Brazil

    PubMed Central

    Prestes-Carneiro, Luiz Euribel; Rubinsky-Elefant, Guita; Ferreira, Antonio Walter; Araujo, Patricia Regina; Troiani, Charlene; Zago, Sueli Cristina; Kaiahara, Marcia; Sasso, Leticia; Iha, Alberto; Vaz, Adelaide

    2013-01-01

    Background The goal of this study was to estimate the seroprevalence of Toxocara spp., Toxoplasma gondii, and Taenia solium metacestode infection and determine some of the associated risk factors for people living in the Dona Carmen settlement, Pontal of Paranapanema, São Paulo, Brazil. Methods Serum samples from 194 subjects were tested and participants answered a questionnaire. An enzyme-linked immunosorbent assay (ELISA) system based on Toxocara spp. excretory-secretory antigens obtained from the cultured second-stage larvae of Toxocara canis or vesicular fluid (VF) antigen from Taenia crassiceps metacestode was used to detect anti-Toxocara spp. IgG and IgE and anti-T. solium metacestode, respectively. For cysticercosis, the reactive ELISA samples were assayed by Western blotting using 18 kDa and 14 kDa proteins purified from VF. For T. gondii-specific IgG and IgM antibodies, anti-SAG-1, GRA-1, and GRA-7 epitope specificity was determined by ELISA. Results Toxoplasma gondii IgG antibodies were found in 102/194 individuals (52.6%) with increased infections in females (P  =  0.02) and those with ≤US$300 monthly income (P  =  0.01). Positive IgM antibodies were detected in 21/194 individuals (10.8%). Antibodies specific to Toxocara spp. were found in 28/194 subjects (14.4%). All the individuals with Toxocara spp. also had T. gondii-specific IgG antibodies. Taenia solium metacestode antibodies were detected in 11 subjects (5.7%), but none were reactive based on Western blotting. Conclusion In spite of environmental, educational, and socioeconomic factors favoring parasite infection, the seropositivity rates of T. gondii, Toxocara spp., and T. solium metacestode-specific IgG antibodies are similar to the rates found in studies conducted in different populations in Brazil. PMID:23683335

  12. Enhanced thermostability of methyl parathion hydrolase from Ochrobactrum sp. M231 by rational engineering of a glycine to proline mutation.

    PubMed

    Tian, Jian; Wang, Ping; Gao, Shan; Chu, Xiaoyu; Wu, Ningfeng; Fan, Yunliu

    2010-12-01

    Protein thermostability can be increased by some glycine to proline mutations in a target protein. However, not all glycine to proline mutations can improve protein thermostability, and this method is suitable only at carefully selected mutation sites that can accommodate structural stabilization. In this study, homology modeling and molecular dynamics simulations were used to select appropriate glycine to proline mutations to improve protein thermostability, and the effect of the selected mutations was proved by the experiments. The structure of methyl parathion hydrolase (MPH) from Ochrobactrum sp. M231 (Ochr-MPH) was constructed by homology modeling, and molecular dynamics simulations were performed on the modeled structure. A profile of the root mean square fluctuations of Ochr-MPH was calculated at the nanosecond timescale, and an eight-amino acid loop region (residues 186-193) was identified as having high conformational fluctuation. The two glycines nearest to this region were selected as mutation targets that might affect protein flexibility in the vicinity. The structures and conformational fluctuations of two single mutants (G194P and G198P) and one double mutant (G194P/G198P) were modeled and analyzed using molecular dynamics simulations. The results predicted that the mutant G194P had the decreased conformational fluctuation in the loop region and might increase the thermostability of Ochr-MPH. The thermostability and kinetic behavior of the wild-type and three mutant enzymes were measured. The results were consistent with the computational predictions, and the mutant G194P was found to have higher thermostability than the wild-type enzyme. © 2010 The Authors Journal compilation © 2010 FEBS.

  13. Allergic conjunctivitis and dry eye syndrome.

    PubMed

    Hom, Milton M; Nguyen, Andrew L; Bielory, Leonard

    2012-03-01

    Allergic conjunctivitis (AC) and dry eye syndrome (DES) are 2 of the most common anterior inflammatory disorders of the ocular surface and one does not preclude the coexistence of the other. To examine the potential overlap between AC and DES as comorbidities. Using the validated questionnaire known as Subjective Evaluation of Symptom of Dryness, we studied self-reported itchiness, dryness, and redness. In an outpatient optometric setting, 689 patients treated from January 1, 2007, to January 1, 2011, were surveyed for their ocular history and categorized according to their reported level of discomfort of itchiness, dryness, and redness. Patients ranged in age from 5 to 90 years (median age, 25 years; 39.5% male; 60.5% female). In the studied 689 patients, clinically significant itchiness was found in 194 (28.2%), dry eyes in 247 (35.8%), and redness in 194 (28.2%). Symptom overlap was demonstrated in many of the patients. Of the 194 patients with itchiness, 112 (57.7%) had clinically significant dryness. In the 247 patients with dry eyes, 112 (45.3%) had clinically significant itch. Redness was apparent in 120 of the 194 patients with itch (61.9%) and 122 of the 247 patients with dryness (49.4%). Statistical analysis demonstrated that self-reported itchiness, dryness, and redness were not independent of each other (P<.001; Pearson χ(2) test). The odds of patients with "itchy eyes" also experiencing dry eyes were 2.11 times and the odds of these patients also experiencing redness were 7.34 times that of patients with nonitchy eyes. Most patients with "itchy eyes" consistent with AC also have dry eyes and redness. These results suggest that some symptomatic patients concomitantly have features of AC and DES. Copyright © 2012 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.

  14. Synthesis and electrochemical characterizations of spinel LiMn1.94MO4 (M = Mn0.06, Mg0.06, Si0.06, (Mg0.03Si0.03)) compounds as cathode materials for lithium-ion batteries

    NASA Astrophysics Data System (ADS)

    Zhao, Hongyuan; Liu, Xingquan; Cheng, Cai; Li, Qiang; Zhang, Zheng; Wu, Yue; Chen, Bing; Xiong, Weiqiang

    2015-05-01

    The spinel LiMn1.94MO4 (M = Mn0.06, Mg0.06, Si0.06, (Mg0.03Si0.03)) compounds are successfully synthesized by citric acid-assisted sol-gel method. The crystal structures and morphologies of synthesized compounds are characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), respectively. All the compounds possess the cubic spinel structure of LiMn2O4 with space group of Fd-3m. The electrochemical properties of synthesized compounds are investigated by galvanostatic charge-discharge test, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results show that the Si-doping can increase the discharge capacity of LiMn2O4 due to the more expanded and regular MnO6 octahedra. In particular, for the LiMn1.94Mg0.03Si0.03O4 compound, the addition of Si4+ ions can make up for the shortage of Mg-doping in term of the discharge capacity. As a result, the Mg2+ and Si4+ co-doping has the effect of synergistic enhancement, which can make full use of the respective advantages of Mg-doping and Si-doping. The optimal LiMn1.94Mg0.03Si0.03O4 can deliver the initial discharge capacity of 128.3 mAh g-1 with good capacity retention of 92.8% after 100 cycles at 0.5 C in the voltage range of 3.20-4.35 V. Compared with the undoped LiMn2O4, the co-doped compound also presents superior rate performance, especially the capacity recovery performance.

  15. GWAS for plant growth stages and yield components in spring wheat (Triticum aestivum L.) harvested in three regions of Kazakhstan.

    PubMed

    Turuspekov, Yerlan; Baibulatova, Aida; Yermekbayev, Kanat; Tokhetova, Laura; Chudinov, Vladimir; Sereda, Grigoriy; Ganal, Martin; Griffiths, Simon; Abugalieva, Saule

    2017-11-14

    Spring wheat is the largest agricultural crop grown in Kazakhstan with an annual sowing area of 12 million hectares in 2016. Annually, the country harvests around 15 million tons of high quality grain. Despite environmental stress factors it is predicted that the use of new technologies may lead to increases in productivity from current levels of 1.5 to up to 3 tons per hectare. One way of improving wheat productivity is by the application of new genomic oriented approaches in plant breeding projects. Genome wide association studies (GWAS) are emerging as powerful tools for the understanding of the inheritance of complex traits via utilization of high throughput genotyping technologies and phenotypic assessments of plant collections. In this study, phenotyping and genotyping data on 194 spring wheat accessions from Kazakhstan, Russia, Europe, and CIMMYT were assessed for the identification of marker-trait associations (MTA) of agronomic traits by using GWAS. Field trials in Northern, Central and Southern regions of Kazakhstan using 194 spring wheat accessions revealed strong correlations of yield with booting date, plant height, biomass, number of spikes per plant, and number of kernels per spike. The accessions from Europe and CIMMYT showed high breeding potential for Southern and Central regions of the country in comparison with the performance of the local varieties. The GGE biplot method, using average yield per plant, suggested a clear separation of accessions into their three breeding origins in relationship to the three environments in which they were evaluated. The genetic variation in the three groups of accessions was further studied using 3245 polymorphic SNP (single nucleotide polymorphism) markers. The application of Principal Coordinate analysis clearly grouped the 194 accessions into three clades according to their breeding origins. GWAS on data from nine field trials allowed the identification of 114 MTAs for 12 different agronomic traits. Field evaluation of foreign germplasm revealed its poor yield performance in Northern Kazakhstan, which is the main wheat growing region in the country. However, it was found that EU and CIMMYT germplasm has high breeding potential to improve yield performance in Central and Southern regions. The use of Principal Coordinate analysis clearly separated the panel into three distinct groups according to their breeding origin. GWAS based on use of the TASSEL 5.0 package allowed the identification of 114 MTAs for twelve agronomic traits. The study identifies a network of key genes for improvement of yield productivity in wheat growing regions of Kazakhstan.

  16. Production and decay spectroscopy of {sup 192}Po and {sup 194}Po

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Andel, B., E-mail: boris.andel@fmph.uniba.sk

    2015-10-15

    A γ-ray spectroscopy study of the (11{sup −}) isomers in {sup 194}Po and {sup 192}Po was performed at the velocity filter SHIP at GSI (Germany). Nuclei were produced in fusion-evaporation reactions and investigated in the detection set-up at the focal plane after the separator. Several new γ transitions were attributed to the isomers and detailed analysis of the first γ-γ coincidences for both isomers is being prepared. For the reaction {sup 56}Fe + {sup 141}Pr → {sup 197}At*, excitation functions for astatine and polonium isotopes were measured and compared with HIVAP calculations.

  17. Evolution of structure and magnetic properties for BaFe11.9Al0.1O19 hexaferrite in a wide temperature range

    NASA Astrophysics Data System (ADS)

    Trukhanov, A. V.; Trukhanov, S. V.; Panina, L. V.; Kostishyn, V. G.; Kazakevich, I. S.; Trukhanov, An. V.; Trukhanova, E. L.; Natarov, V. O.; Turchenko, V. A.; Salem, M. M.; Balagurov, A. M.

    2017-03-01

    M-type BaFe11.9Al0.1O19 hexaferrite was successfully synthesized by solid state reactions. Precision investigations of crystal and magnetic structures of BaFe11.9Al0.1O19 powder by neutron diffraction in the temperature range 4.2-730 K have been performed. Magnetic and electrical properties investigations were carried out in the wide temperature range. Neutron powder diffraction data were successfully refined in approximation for both space groups (SG): centrosymmetric #194 (standard non-polar phase) and non-centrosymmetric #186 (polar phase). It has been shown that at low temperatures (below room temperature) better fitting results (value χ2) were for the polar phase (SG: #186) or for the two phases coexistence (SG: #186 and SG: #194). At high temperatures (400-730 K) better fitting results were for SG: #194. It was established coexistence of the dual ferroic properties (specific magnetization and spontaneous polarization) at room temperature. Strong correlation between magnetic and electrical subsystems was demonstrated (magnetoelectrical effect). Temperature dependences of the spontaneous polarization, specific magnetization and magnetoelectrical effect were investigated.

  18. miRNA-216 and miRNA-499 target cyb561d2 in zebrafish in response to fipronil exposure.

    PubMed

    Zhou, Yongyong; Huang, Hannian; Zhang, Kai; Ding, Xianfeng; Jia, Longlue; Yu, Liang; Zhu, Guonian; Guo, Jiangfeng

    2016-07-01

    MicroRNA (miRNA) can regulate the expression of its target gene by mediating mRNA cleavage or by translational repression at a post-transcriptional level. Usually, one miRNA may regulate many genes as its targets, while one gene may also be targeted by many miRNAs. We previously demonstrated that cyb561d2, whose protein product is involved in cell defense, and chemical stress, is targeted by miR-155 in adult zebrafish (Danio rerio) when exposed to fipronil (5-amino-1-[2,6-dichloro-4-(trifluoromethyl) phenyl]-4-[(trifluoromethyl) sulphinyl]-1H-pyrazole-3-carbonitrile). Microcosm Targets prediction showed that the cyb561d2 gene is also highly possibly targeted by miR-194a, miR-216b, miR-429, and miR-499. These interactions need to be further validated experimentally. In this study, we evaluated the effects of fipronil on miR-194a, miR-216b, miR-429, miR-499 and cyb561d2 in zebrafish and investigated whether these four miRNAs could regulate the expression of cyb561d2 in both mRNA and protein levels. The expression of cyb561d2 was upregulated in both mRNA and protein level in a dose-dependent manner upon stimulation of fipronil, and miR-216b and miR-499 were downregulated concurrently, whereas there was no significant changes were observed in the expression level of miR-194a and miR-429. The dual luciferase report assay demonstrated that miR-216b and miR-499 interacted with cyb561d2 3'-untranslated regions (3'-UTR), miR-194a and miR-429 did not stimulate degradation of cyb561d2 mRNA. The expression of cyb561d2 was reduced in both mRNA and protein level when ZF4 cells were transfected with miR-499 mimic, whereas expression level of both mRNA and protein was increased when endogenous miR-499 was inhibited by transfection with miR-499 inhibitor. Likewise, the mRNA and protein level of cyb561d2 was affected by treatment with the mimics and the inhibitor of miR-216b. In contrast, when ZF4 cells were transfected with a mimic of miR-194a or miR-429, the expression of cyb561d2 mRNA was not significantly changed. As a result, cyb561d2 is targeted by miR-155, miR-216b and miR-499 upon fipronil exposure, and miR-194a and miR-429 can not target cyb561d2. The expression pattern of these 3 miRNAs presents novel fipronil responses that could be used as a toxicological biomarker. Copyright © 2016 Elsevier B.V. All rights reserved.

  19. Relationship between blood levels of heavy metals and lung function based on the Korean National Health and Nutrition Examination Survey IV–V

    PubMed Central

    Leem, Ah Young; Kim, Se Kyu; Chang, Joon; Kang, Young Ae; Kim, Young Sam; Park, Moo Suk; Kim, Song Yee; Kim, Eun Young; Chung, Kyung Soo; Jung, Ji Ye

    2015-01-01

    Background Heavy metal exposure may contribute to inflammation in the lungs via increased oxidative stress, resulting in tissue destruction and obstructive lung function (OLF). In this study, we evaluated the relationship between lead and cadmium levels in blood, and lung function in the Korean population. Methods Pooled cross-sectional data from 5,972 subjects who participated in the Korean National Health and Nutrition Examination Survey 2008–2012 were used for this study. OLF was defined as forced expiratory volume in 1 second/forced vital capacity (FEV1/FVC) <0.7. Graphite-furnace atomic absorption spectrometry was used to measure levels of lead and cadmium in blood. Results Adjusted means for age, sex, body mass index, and smoking status in blood lead and cadmium levels were increased with age and were higher in men and current smokers. The FEV1/FVC ratio was lower in the highest quartile group of lead (78.4% vs 79.0%; P=0.025) and cadmium (78.3% vs 79.2%; P<0.001) concentrations, compared with those in the lowest quartile groups. Multiple linear regression demonstrated an inverse relationship between the FEV1/FVC ratio and concentrations of lead (estimated −0.002; P=0.007) and cadmium (estimated −0.005; P=0.001). Of the 5,972 subjects, 674 (11.3%) were classified into the OLF group. Among current smokers, the risk of OLF was higher in subjects in the highest quartile group of cadmium concentration than in those in the lowest quartile group (odds ratio 1.94; 95% confidence interval 1.06–3.57). Conclusion We demonstrated a significant association between the FEV1/FVC ratio and blood concentrations of lead and cadmium in the Korean population. The risk for OLF was elevated with increasing concentrations of cadmium among current smokers. PMID:26345298

  20. Alloy-steel nuts for bolting for high-pressure and high-temperature service (ASME SA-194 with additional requirements)

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Not Available

    This standard covers alloy steel nuts for bolting for high-pressure and high-temperature service in nuclear and associated applications. This standard does not cover bar or other starting materials. The only implied special considerations for starting materials are that they be capable of passing the required tests when processed into finished products in accordance with this standard. Material shall conform to the requirements of ASME SA-194; to the requirements of the ASME Boiler and Pressure Vessel Code (ASME Code), Section III, Article NB-2000; to the requirements of NE E 8-18; and to the additional requirements of this standard.

  1. Detection of moisture and moisture related phenomena from Skylab. [correlation of S-194 antenna temperature and soil moisture content measurements for Kansas and Texas

    NASA Technical Reports Server (NTRS)

    Eagleman, J. R.; Pogge, E. C.; Moore, R. K. (Principal Investigator); Hardy, N.; Lin, W.; League, L.

    1973-01-01

    The author has identified the following significant results. Correlations between the S-194 antenna temperature and soil moisture have been obtained for three sets of data; one for Skylab 2 and two for Skylab 3. The best correlations were obtained for the surface to one inch depth in two cases and for the surface to two inches for the third case. Correlation coefficients for the surface to one inch depth were -0.98, -0.95, and -0.82. The lowest correlation coefficient was obtained with total soil moisture variations less than 4% across the test site.

  2. The common causes leading to pancytopenia in patients presenting to tertiary care hospital.

    PubMed

    Das Makheja, Kirpal; Kumar Maheshwari, Bharat; Arain, Shafique; Kumar, Suneel; Kumari, Sangeeta; Vikash

    2013-09-01

    The objective of this study was to determine the frequency of common causes leading to Pancytopenia in patients presenting to tertiary care hospital at Karachi. A total of 62 patients with the diagnosis of Pancytopenia of more than one week duration were enrolled in the study. All patients underwent a detailed medical history and full physical examination followed by blood sampling for the investigations i.e. complete blood count with peripheral film, erythrocyte sedimentation rate (ESR), Malarial parasites (MP), liver function test, Renal function tests, PT and viral profile (HBsAg, Anti-HCV), Ultrasonography of abdomen. All patients underwent bone marrow aspiration and trephine biopsy for reporting and interpretation. Duration of study was six months, from May 2010 to November 2010. The average age of the patients was 37.76 ± 16.38years. Out of 62 patients, 36 (58%) were male and 26 (42%) were female. Megaloblastic anemia was the commonest cause that was observed in 41.9% cases followed by acute myeloid leukemia 27.4%, aplastic anemia 19.4% and erythroid hyperplasia 11.3%. Conclusion : This study concluded that most common cause of pancytopenia is Megaloblastic anemia, followed by acute myeloid leukemia and aplastic anemia. Bone marrow examination is a single useful investigation which reveals the underlying cause in patients with pancytopenia.

  3. The determination of mercury in mushrooms by CV-AAS and ICP-AES techniques.

    PubMed

    Jarzynska, Grazyna; Falandysz, Jerzy

    2011-01-01

    This research presents an example of an excellent applied study on analytical problems due to hazardous mercury determination in environmental materials and validity of published results on content of this element in wild growing mushrooms. The total mercury content has been analyzed in a several species of wild-grown mushrooms and some herbal origin certified reference materials, using two analytical methods. One method was commonly known and well validated the cold-vapour atomic absorption spectroscopy (CV-AAS) after a direct sample pyrolysis coupled to the gold wool trap, which was a reference method. A second method was a procedure that involved a final mercury measurement using the inductively-coupled plasma atomic emission spectroscopy (ICP-AES) at λ 194.163 nm, which was used by some authors to report on a high mercury content of a large sets of wild-grown mushrooms. We found that the method using the ICP-AES at λ 194.163 nm gave inaccurate and imprecise results. The results of this study imply that because of unsuitability of total mercury determination using the ICP-AES at λ 194.163 nm, the reports on great concentrations of this metal in a large sets of wild-grown mushrooms, when examined using this method, have to be studied with caution, since data are highly biased.

  4. H1PVAT is a novel and potent early-stage inhibitor of poliovirus replication that targets VP1.

    PubMed

    Tijsma, Aloys; Thibaut, Hendrik Jan; Spieser, Stéphane A H; De Palma, Armando; Koukni, Mohamed; Rhoden, Eric; Oberste, Steve; Pürstinger, Gerhard; Volny-Luraghi, Antonia; Martin, Javier; Marchand, Arnaud; Chaltin, Patrick; Neyts, Johan; Leyssen, Pieter

    2014-10-01

    A novel small molecule, H1PVAT, was identified as a potent and selective inhibitor of the in vitro replication of all three poliovirus serotypes, whereas no activity was observed against other enteroviruses. Time-of-drug-addition studies revealed that the compound interfered with an early stage of virus replication. Four independently-selected H1PVAT-resistant virus variants uniformly carried the single amino acid substitution I194F in the VP1 capsid protein. Poliovirus type 1 strain Sabin, reverse-engineered to contain this substitution, proved to be completely insensitive to the antiviral effect of H1PVAT and was cross-resistant to the capsid-binding inhibitors V-073 and pirodavir. The VP1 I194F mutant had a smaller plaque phenotype than wild-type virus, and the amino acid substitution rendered the virus more susceptible to heat inactivation. Both for the wild-type and VP1 I194F mutant virus, the presence of H1PVAT increased the temperature at which the virus was inactivated, providing evidence that the compound interacts with the viral capsid, and that capsid stabilization and antiviral activity are not necessarily correlated. Molecular modeling suggested that H1PVAT binds with high affinity in the pocket underneath the floor of the canyon that is involved in receptor binding. Introduction of the I194F substitution in the model of VP1 induced a slight concerted rearrangement of the core β-barrel in this pocket, which disfavors binding of the compound. Taken together, the compound scaffold, to which H1PVAT belongs, may represent another promising class of poliovirus capsid-binding inhibitors next to V-073 and pirodavir. Potent antivirals against poliovirus will be essential in the poliovirus eradication end-game. Copyright © 2014. Published by Elsevier B.V.

  5. Polymorphism of the NFKB1 affects the serum inflammatory levels of IL-6 in Hashimoto thyroiditis in a Turkish population.

    PubMed

    Koc, Arzuhan; Batar, Bahadir; Celik, Ozlem; Onaran, Ilhan; Tasan, Ertugrul; Sultuybek, Gonul Kanigur

    2014-07-01

    Hashimoto thyroiditis (HT) is a chronic inflammatory autoimmune disease of thyroid gland affected by interaction of multiple genes and various cytokines. Variants in the genes coding for the NFKB and IKB proteins can be potentially involved in the development of the inflammatory diseases. NFKB, a key transcription factor of the regulation of immune responses, is interesting candidate for association studies about autoimmune disorder. The aim of the present study was to investigate the relationship between NFKB1 and NFKBIA (NFKB1 inhibitor gene) polymorphisms, and the risk of HT in a Turkish Population in the context of IL-6 serum levels which may contribute to susceptibility to the disease. We analyzed the distribution of NFKB1-94ins/del ATTG and NFKBIA 3'UTR A→G polymorphisms using PCR-RFLP method and IL-6 serum levels using ELISA method in 120 HT patients and 190 healthy controls in Turkish population. Although, there was no statistical significant difference in distribution of the genotypes and alleles of NFKB1-94ins/del ATTG or NFKBIA 3'UTR A→G polymorphisms in patients and control subjects as single, ins/ins/GG combined genotype had protective effect on the disease when compared to ins/ins/AG combined genotype as combined genotypes of both polymorphisms. In addition to this finding, IL-6 serum levels in HT patients with del/del genotype were significantly higher than in patients with del/ins genotype (p<0.001). According to the combined genotype analysis of NFKB1-94ins/del ATTG and NFKBIA 3'UTR A→G polymorphisms, IL-6 levels were also higher in patients with del/del genotype when at least one G allele existing (p=0.007). Therefore, our findings suggest that the functional promoter NFKB1-94ins/del ATTG polymorphism was significantly associated with population HT disease through acting by directly modulating IL-6 serum levels. Copyright © 2014 Elsevier GmbH. All rights reserved.

  6. Annual Change Report 2006/2007

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Washington Regulatory and Environmental Services; Washington TRU Solutions LLC

    2007-11-16

    As part of continuing compliance, the U.S. Environmental Protection Agency (EPA) requires the U.S. Department of Energy (DOE) to provide information on any change in conditions or activities pertaining to the disposal system since the most recent compliance application. This requirement is identified in Title 40 Code of Federal Regulations (CFR), Section 194.4(b)(4), which states: "No later than six months after the administrator issues a certification, and at least annually thereafter, the Department shall report to the Administrator, in writing, any changes in conditions or activities pertaining to the disposal system that were not required to be reported by paragraphmore » (b)(3) of this section and that differ from information contained in the most recent compliance application." In meeting the requirement, the DOE provides an annual report each November of all applicable changes under the above requirement. This annual report informs the EPA of changes to information in the most recent compliance recertification (the 2004 Compliance Recertification). Significant planned changes must be reported to the EPA prior to implementation by the DOE. In addition, Title 40 CFR, Section 194.4(b)(3) requires that significant unplanned changes be reported to the EPA within 24 hours or ten days, depending on the severity of the activity or condition. To date, there have been no significant unplanned changes to the certification basis. Planned changes have been submitted on an individual basis. All other changes are reported annually. Changes in activities or conditions are reviewed to determine if 40 CFR Section 194.4(b)(3) reporting is necessary. As indicated above, no significant unplanned changes were identified for the time period covered by this report. The enclosed tables list those items identified for reporting under 40 CFR Section 194.4(b)(4). The majority of the items described in this report are inspections, reports, and modifications to written plans and procedures for WIPP operations.« less

  7. Under-prescribing of Prevention Drugs and Primary Prevention of Stroke and Transient Ischaemic Attack in UK General Practice: A Retrospective Analysis.

    PubMed

    Turner, Grace M; Calvert, Melanie; Feltham, Max G; Ryan, Ronan; Fitzmaurice, David; Cheng, K K; Marshall, Tom

    2016-11-01

    Stroke is a leading cause of death and disability; worldwide it is estimated that 16.9 million people have a first stroke each year. Lipid-lowering, anticoagulant, and antihypertensive drugs can prevent strokes, but may be underused. We analysed anonymised electronic primary care records from a United Kingdom (UK) primary care database that covers approximately 6% of the UK population. Patients with first-ever stroke/transient ischaemic attack (TIA), ≥18 y, with diagnosis between 1 January 2009 and 31 December 2013, were included. Drugs were considered under-prescribed when lipid-lowering, anticoagulant, or antihypertensive drugs were clinically indicated but were not prescribed prior to the time of stroke or TIA. The proportions of strokes or TIAs with prevention drugs under-prescribed, when clinically indicated, were calculated. In all, 29,043 stroke/TIA patients met the inclusion criteria; 17,680 had ≥1 prevention drug clinically indicated: 16,028 had lipid-lowering drugs indicated, 3,194 anticoagulant drugs, and 7,008 antihypertensive drugs. At least one prevention drug was not prescribed when clinically indicated in 54% (9,579/17,680) of stroke/TIA patients: 49% (7,836/16,028) were not prescribed lipid-lowering drugs, 52% (1,647/3,194) were not prescribed anticoagulant drugs, and 25% (1,740/7,008) were not prescribed antihypertensive drugs. The limitations of our study are that our definition of under-prescribing of drugs for stroke/TIA prevention did not address patients' adherence to medication or medication targets, such as blood pressure levels. In our study, over half of people eligible for lipid-lowering, anticoagulant, or antihypertensive drugs were not prescribed them prior to first stroke/TIA. We estimate that approximately 12,000 first strokes could potentially be prevented annually in the UK through optimal prescribing of these drugs. Improving prescription of lipid-lowering, anticoagulant, and antihypertensive drugs is important to reduce the incidence and burden of stroke and TIA.

  8. Enterovirus 71 Inhibits Pyroptosis through Cleavage of Gasdermin D

    PubMed Central

    Lei, Xiaobo; Zhang, Zhenzhen; Xiao, Xia; Qi, Jianli

    2017-01-01

    ABSTRACT Enterovirus 71 (EV71) can cause hand-foot-and-mouth disease (HFMD) in young children. Severe infection with EV71 can lead to neurological complications and even death. However, the molecular basis of viral pathogenesis remains poorly understood. Here, we report that EV71 induces degradation of gasdermin D (GSDMD), an essential component of pyroptosis. Remarkably, the viral protease 3C directly targets GSDMD and induces its cleavage, which is dependent on the protease activity. Further analyses show that the Q193-G194 pair within GSDMD is the cleavage site of 3C. This cleavage produces a shorter N-terminal fragment spanning amino acids 1 to 193 (GSDMD1–193). However, unlike the N-terminal fragment produced by caspase-1 cleavage, this fragment fails to trigger cell death or inhibit EV71 replication. Importantly, a T239D or F240D substitution abrogates the activity of GSDMD consisting of amino acids 1 to 275 (GSDMD1–275). This is correlated with the lack of pyroptosis or inhibition of viral replication. These results reveal a previously unrecognized strategy for EV71 to evade the antiviral response. IMPORTANCE Recently, it has been reported that GSDMD plays a critical role in regulating lipopolysaccharide and NLRP3-mediated interleukin-1β (IL-1β) secretion. In this process, the N-terminal domain of p30 released from GSDMD acts as an effector in cell pyroptosis. We show that EV71 infection downregulates GSDMD. EV71 3C cleaves GSDMD at the Q193-G194 pair, resulting in a truncated N-terminal fragment disrupted for inducing cell pyroptosis. Notably, GSDMD1–275 (p30) inhibits EV71 replication whereas GSDMD1–193 does not. These results reveal a new strategy for EV71 to evade the antiviral response. PMID:28679757

  9. Enterovirus 71 Inhibits Pyroptosis through Cleavage of Gasdermin D.

    PubMed

    Lei, Xiaobo; Zhang, Zhenzhen; Xiao, Xia; Qi, Jianli; He, Bin; Wang, Jianwei

    2017-09-15

    Enterovirus 71 (EV71) can cause hand-foot-and-mouth disease (HFMD) in young children. Severe infection with EV71 can lead to neurological complications and even death. However, the molecular basis of viral pathogenesis remains poorly understood. Here, we report that EV71 induces degradation of gasdermin D (GSDMD), an essential component of pyroptosis. Remarkably, the viral protease 3C directly targets GSDMD and induces its cleavage, which is dependent on the protease activity. Further analyses show that the Q193-G194 pair within GSDMD is the cleavage site of 3C. This cleavage produces a shorter N-terminal fragment spanning amino acids 1 to 193 (GSDMD 1-193 ). However, unlike the N-terminal fragment produced by caspase-1 cleavage, this fragment fails to trigger cell death or inhibit EV71 replication. Importantly, a T239D or F240D substitution abrogates the activity of GSDMD consisting of amino acids 1 to 275 (GSDMD 1-275 ). This is correlated with the lack of pyroptosis or inhibition of viral replication. These results reveal a previously unrecognized strategy for EV71 to evade the antiviral response. IMPORTANCE Recently, it has been reported that GSDMD plays a critical role in regulating lipopolysaccharide and NLRP3-mediated interleukin-1β (IL-1β) secretion. In this process, the N-terminal domain of p30 released from GSDMD acts as an effector in cell pyroptosis. We show that EV71 infection downregulates GSDMD. EV71 3C cleaves GSDMD at the Q193-G194 pair, resulting in a truncated N-terminal fragment disrupted for inducing cell pyroptosis. Notably, GSDMD 1-275 (p30) inhibits EV71 replication whereas GSDMD 1-193 does not. These results reveal a new strategy for EV71 to evade the antiviral response. Copyright © 2017 American Society for Microbiology.

  10. Using vertebral movement and intact paraspinal muscles to determine the distribution of intrafusal fiber innervation of muscle spindle afferents in the anesthetized cat.

    PubMed

    Reed, William R; Cao, Dong-Yuan; Ge, Weiqing; Pickar, Joel G

    2013-03-01

    Increasing our knowledge regarding intrafusal fiber distribution and physiology of paraspinal proprioceptors may provide key insights regarding proprioceptive deficits in trunk control associated with low back pain and lead to more effective clinical intervention. The use of vertebral movement as a means to reliably stretch paraspinal muscles would greatly facilitate physiological study of paraspinal muscle proprioceptors where muscle tendon isolation is either very difficult or impossible. The effects of succinylcholine (SCh) on 194 muscle spindle afferents from lumbar longissimus or multifidus muscles in response to computer-controlled, ramp-and-hold movements of the L(6) vertebra were investigated in anesthetized cats. Paraspinal muscles were stretched by moving the L(6) vertebra 1.5-1.7 mm in the dorsal-ventral direction. Initial frequency (IF), dynamic difference (DD), their changes (∆) following SCh injection (100-400 μg kg(-1)), and post-SCh dynamic difference (SChDD) were measured. Muscle spindle intrafusal fiber terminations were classified as primary or secondary fibers as well as bag(1) (b(1)c), bag(2) (b(2)c), b(1)b(2)c, or chain (c) fibers. Intrafusal fiber subpopulations were distinguished using logarithmic transformation of SChDD and ∆IF distributions as established by previous investigators. Increases in DD indicate strength of b(1)c influence while increases in IF indicate strength of b(2)c influence. Out of 194 afferents, 46.9 % of afferents terminated on b(2)c fibers, 46.4 % on b(1)b(2)c fibers, 1 % on b(1)c fibers, and 5.7 % terminated on c fibers. Based on these intrafusal fiber subpopulation distributions, controlled vertebral movement can effectively substitute for direct tendon stretch and allow further investigation of paraspinal proprioceptors in this anatomically complex body region.

  11. Columbus AFB, Mississippi. Revised Uniform Summary of Surface Weather Observations (RUSSWO). Parts A-F.

    DTIC Science & Technology

    1982-02-16

    E~ __ _ 1.0 a 3 ____ __ _ 1.4 3I. 4 ENE 1.7 . . .1 4 *1 2 1 .9 5. ,e i 101 * ’ 3 e!. 203 4.3 IE 1 1.6 1.9 . 4 3.9 .3_8 Se 1.9_ It.7 194 .3 1___...SERVICL/I4AC PERCENTAGE FREQUENCY OF WINDSU FC WID DIRECTION AND SPEED (FROM HOURLY OBSERVATIONS) .23 COLUMBUS AFB MS 7C73-1__ _____ $TAT"S STATION...1 3 4 __ _ I _ __________. NNE 1.4 .2 eI _ _ _. It? 29__8 NE .8 .3 1,__ _ _ 2.s ENE .8 . _ 1 .1 194 . _,_ 395E i . 7 . 8 . l i 2 .6: , 3 . 1 ESE 4.7

  12. A comparison of radiative transfer models for predicting the microwave emission from soils

    NASA Technical Reports Server (NTRS)

    Schmugge, T. J.; Choudhury, B. J.

    1981-01-01

    Noncoherent and coherent numerical models for predicting emission from soils are compared. Coherent models use the boundary conditions on the electric fields across the layer boundaries to calculate the radiation intensity, and noncoherent models consider radiation intensities directly. Interference may cause different results in the two approaches when coupling between soil layers in coherent models causes greater soil moisture sampling depths. Calculations performed at frequencies of 1.4 and 19.4 GHz show little difference between the models at 19.4 GHz, although differences are apparent at the lower frequency. A definition for an effective emissivity is also given for when a nonuniform temperature profile is present, and measurements made from a tower show good agreement with calculations from the coherent model.

  13. Measurement of D ∗ meson cross sections at HERA and determination of the gluon density in the proton using NLO QCD

    NASA Astrophysics Data System (ADS)

    Adloff, C.; Anderson, M.; Andreev, V.; Andrieu, B.; Arkadov, V.; Arndt, C.; Ayyaz, I.; Babaev, A.; Bähr, J.; Baranov, P.; Barrelet, E.; Bartel, W.; Bassler, U.; Bate, P.; Beck, M.; Beglarian, A.; Behnke, O.; Behrend, H.-J.; Beier, C.; Belousov, A.; Berger, Ch.; Bernardi, G.; Bertrand-Coremans, G.; Biddulph, P.; Bizot, J. C.; Boudry, V.; Braunschweig, W.; Brisson, V.; Brown, D. P.; Brückner, W.; Bruel, P.; Bruncko, D.; Bürger, J.; Büsser, F. W.; Buniatian, A.; Burke, S.; Burrage, A.; Buschhorn, G.; Calvet, D.; Campbell, A. J.; Carli, T.; Chabert, E.; Charlet, M.; Clarke, D.; Clerbaux, B.; Contreras, J. G.; Cormack, C.; Coughlan, J. A.; Cousinou, M.-C.; Cox, B. E.; Cozzika, G.; Cvach, J.; Dainton, J. B.; Dau, W. D.; Daum, K.; David, M.; Davidsson, M.; De Roeck, A.; De Wolf, E. A.; Delcourt, B.; Demirchyan, R.; Diaconu, C.; Dirkmann, M.; Dixon, P.; Dlugosz, W.; Donovan, K. T.; Dowell, J. D.; Droutskoi, A.; Ebert, J.; Eckerlin, G.; Eckstein, D.; Efremenko, V.; Egli, S.; Eichler, R.; Eisele, F.; Eisenhandler, E.; Elsen, E.; Enzenberger, M.; Erdmann, M.; Fahr, A. B.; Favart, L.; Fedotov, A.; Felst, R.; Feltesse, J.; Ferencei, J.; Ferrarotto, F.; Fleischer, M.; Flügge, G.; Fomenko, A.; Formánek, J.; Foster, J. M.; Franke, G.; Gabathuler, E.; Gabathuler, K.; Gaede, F.; Garvey, J.; Gassner, J.; Gayler, J.; Gerhards, R.; Ghazaryan, S.; Glazov, A.; Goerlich, L.; Gogitidze, N.; Goldberg, M.; Gorelov, I.; Grab, C.; Grässler, H.; Greenshaw, T.; Griffiths, R. K.; Grindhammer, G.; Hadig, T.; Haidt, D.; Hajduk, L.; Haller, T.; Hampel, M.; Haustein, V.; Haynes, W. J.; Heinemann, B.; Heinzelmann, G.; Henderson, R. C. W.; Hengstmann, S.; Henschel, H.; Heremans, R.; Herynek, I.; Hewitt, K.; Hiller, K. H.; Hilton, C. D.; Hladký, J.; Hoffmann, D.; Horisberger, R.; Hurling, S.; Ibbotson, M.; İşsever, Ç.; Jacquet, M.; Jaffre, M.; Jansen, D. M.; Jönsson, L.; Johnson, D. P.; Jones, M.; Jung, H.; Kästli, H. K.; Kander, M.; Kant, D.; Kapichine, M.; Karlsson, M.; Karschnik, O.; Katzy, J.; Kaufmann, O.; Kausch, M.; Kenyon, I. R.; Kermiche, S.; Keuker, C.; Kiesling, C.; Klein, M.; Kleinwort, C.; Knies, G.; Köhne, J. H.; Kolanoski, H.; Kolya, S. D.; Korbel, V.; Kostka, P.; Kotelnikov, S. K.; Krämerkämper, T.; Krasny, M. W.; Krehbiel, H.; Krücker, D.; Krüger, K.; Küpper, A.; Küster, H.; Kuhlen, M.; Kurča, T.; Lahmann, R.; Landon, M. P. J.; Lange, W.; Langenegger, U.; Lebedev, A.; Lehner, F.; Lemaitre, V.; Lendermann, V.; Levonian, S.; Lindstroem, M.; List, B.; Lobo, G.; Lobodzinska, E.; Lubimov, V.; Lüders, S.; Lüke, D.; Lytkin, L.; Magnussen, N.; Mahlke-Krüger, H.; Malinovski, E.; Maraček, R.; Marage, P.; Marks, J.; Marshall, R.; Martin, G.; Martyn, H.-U.; Martyniak, J.; Maxfield, S. J.; McMahon, T. R.; Mehta, A.; Meier, K.; Merkel, P.; Metlica, F.; Meyer, A.; Meyer, A.; Meyer, H.; Meyer, J.; Meyer, P.-O.; Mikocki, S.; Milstead, D.; Moeck, J.; Mohr, R.; Mohrdieck, S.; Moreau, F.; Morris, J. V.; Müller, D.; Müller, K.; Murin, P.; Nagovizin, V.; Naroska, B.; Naumann, Th.; Négri, I.; Newman, P. R.; Nguyen, H. K.; Nicholls, T. C.; Niebergall, F.; Niebuhr, C.; Niedzballa, Ch.; Niggli, H.; Nikitin, D.; Nix, O.; Nowak, G.; Nunnemann, T.; Oberlack, H.; Olsson, J. E.; Ozerov, D.; Palmen, P.; Panassik, V.; Pascaud, C.; Passaggio, S.; Patel, G. D.; Pawletta, H.; Perez, E.; Phillips, J. P.; Pieuchot, A.; Pitzl, D.; Pöschl, R.; Pope, G.; Povh, B.; Rabbertz, K.; Rauschenberger, J.; Reimer, P.; Reisert, B.; Reyna, D.; Rick, H.; Riess, S.; Rizvi, E.; Robmann, P.; Roosen, R.; Rosenbauer, K.; Rostovtsev, A.; Rouse, F.; Royon, C.; Rusakov, S.; Rybicki, K.; Sankey, D. P. C.; Schacht, P.; Scheins, J.; Schilling, F.-P.; Schleif, S.; Schleper, P.; Schmidt, D.; Schmidt, D.; Schoeffel, L.; Schröder, V.; Schultz-Coulon, H.-C.; Schwab, B.; Sefkow, F.; Semenov, A.; Shekelyan, V.; Sheviakov, I.; Shtarkov, L. N.; Siegmon, G.; Sirois, Y.; Sloan, T.; Smirnov, P.; Smith, M.; Solochenko, V.; Soloviev, Y.; Spaskov, V.; Specka, A.; Spiekermann, J.; Spitzer, H.; Squinabol, F.; Steffen, P.; Steinberg, R.; Steinhart, J.; Stella, B.; Stellberger, A.; Stiewe, J.; Straumann, U.; Struczinski, W.; Sutton, J. P.; Swart, M.; Tapprogge, S.; Taševský, M.; Tchernshov, V.; Tchetchelnitski, S.; Theissen, J.; Thompson, G.; Thompson, P. D.; Tobien, N.; Todenhagen, R.; Truöl, P.; Tsipolitis, G.; Turnau, J.; Tzamariudaki, E.; Udluft, S.; Usik, A.; Valkár, S.; Valkárová, A.; Vallée, C.; Van Esch, P.; Van Haecke, A.; Van Mechelen, P.; Vazdik, Y.; Villet, G.; Wacker, K.; Wallny, R.; Walter, T.; Waugh, B.; Weber, G.; Weber, M.; Wegener, D.; Wegner, A.; Wengler, T.; Werner, M.; West, L. R.; Wiesand, S.; Wilksen, T.; Willard, S.; Winde, M.; Winter, G.-G.; Wittek, C.; Wittmann, E.; Wobisch, M.; Wollatz, H.; Wünsch, E.; Žaček, J.; Zálešak, J.; Zhang, Z.; Zhokin, A.; Zini, P.; Zomer, F.; Zsembery, J.; zurNedden, M.; H1 Collaboration

    1999-04-01

    With the H1 detector at the ep collider HERA, D ∗ meson production cross sections have been measured in deep inelastic scattering with four-momentum transfers Q2 > 3 GeV 2 and in photoproduction at energies around Wγp ≈ 88 GeV and 194 GeV. Next-to-Leading Order QCD calculations are found to describe the differential cross sections within theoretical and experimental uncertainties. Using these calculations, the NLO gluon momentum distribution in the proton, xgg( xg), has been extracted in the momentum fraction range 7.5 × 10 -4 < xg < 4 × 10 -2 at average scales μ2 = 25 to 50 GeV 2. The gluon momentum fraction xg has been obtained from the measured kinematics of the scattered electron and the D ∗ meson in the final state. The results compare well with the gluon distribution obtained from the analysis of scaling violations of the proton structure function F2.

  14. Hydrodynamic effects in laser cutting of biological tissue phantoms

    NASA Astrophysics Data System (ADS)

    Zhigarkov, V. S.; Yusupov, V. I.; Tsypina, S. I.; Bagratashvili, V. N.

    2017-11-01

    We study the thermal and transport processes that occur in the course of incision formation at the surface of a biological tissue phantom under the action of near-IR, moderate-power, continuous-wave laser radiation (λ = 1.94 μm) delivered by means of an optical fibre with an absorbing coating on its exit face. It is shown that in addition to the thermal effect, the laser-induced hydrodynamic effects caused by the explosive boiling of the interstitial water make a large contribution to the phantom destruction mechanism. These effects lead to the tissue rupture accompanied by the ejection of part of the fragmented substance from the site of laser impact and the formation of highly porous structure near the incision surface. We have found that the depth, the width and the relief of the laser incision wall in the case of using the optical fibre moving with a constant velocity, depend on the fibre tilt angle with respect to the phantom surface, as well as the direction of the fibre motion.

  15. Emotional Intelligence Mediates the Relationship between Age and Subjective Well-Being.

    PubMed

    Chen, Yiwei; Peng, Yisheng; Fang, Ping

    2016-07-01

    Individuals' Subjective Well-being (SWB) increases as they grow older. Past literature suggests that emotional intelligence may increase with age and lead to higher levels of SWB in older adults. The primary purpose of the present study was to test whether emotional intelligence would mediate the relationship between age and SWB. A total of 360 Chinese adults (age range: 20 to 79 years old) participated in this study. They filled out questionnaires that assessed their age, life satisfaction (The Satisfaction with Life Scale), affective well-being (The Positive and Negative Affect Schedule), and emotional intelligence (The Wong and Law Emotional Intelligence Scale). Using Structural Equation Modeling, the mediation model was supported, χ(2) (75) = 194.21, p < .01; RMSEA = .07; CFI = .91. Emotional intelligence partially mediated the relationship between age and life satisfaction, and fully mediated the relationship between age and affective well-being. The findings suggest that older adults may use their increased emotional intelligence to enhance their SWB. © The Author(s) 2016.

  16. Emotional Intelligence Mediates the Relationship between Age and Subjective Well-Being

    PubMed Central

    Chen, Yiwei; Peng, Yisheng; Fang, Ping

    2017-01-01

    Individuals’ Subjective Well-being (SWB) increases as they grow older. Past literature suggests that emotional intelligence may increase with age and lead to higher levels of SWB in older adults. The primary purpose of the present study was to test whether emotional intelligence would mediate the relationship between age and SWB. A total of 360 Chinese adults (age range: 20 to 79 years old) participated in this study. They filled out questionnaires that assessed their age, life satisfaction (The Satisfaction with Life Scale), affective well-being (The Positive and Negative Affect Schedule), and emotional intelligence (The Wong and Law Emotional Intelligence Scale). Using Structural Equation Modeling, the mediation model was supported, χ2 (75) =194.21, p < .01; RMSEA =.07; CFI = .91. Emotional intelligence partially mediated the relationship between age and life satisfaction, and fully mediated the relationship between age and affective well-being. The findings suggest that older adults may use their increased emotional intelligence to enhance their SWB. PMID:27199490

  17. 40 CFR 194.42 - Monitoring.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., including porosity, permeability, and degree of compaction and reconsolidation; (2) Stresses and extent of..., composition, and spatial distribution; (6) Gas quantity and composition; and (7) Temperature distribution. (b...

  18. 40 CFR 194.42 - Monitoring.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., including porosity, permeability, and degree of compaction and reconsolidation; (2) Stresses and extent of..., composition, and spatial distribution; (6) Gas quantity and composition; and (7) Temperature distribution. (b...

  19. 40 CFR 194.42 - Monitoring.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., including porosity, permeability, and degree of compaction and reconsolidation; (2) Stresses and extent of..., composition, and spatial distribution; (6) Gas quantity and composition; and (7) Temperature distribution. (b...

  20. 40 CFR 194.42 - Monitoring.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., including porosity, permeability, and degree of compaction and reconsolidation; (2) Stresses and extent of..., composition, and spatial distribution; (6) Gas quantity and composition; and (7) Temperature distribution. (b...

  1. 40 CFR 194.42 - Monitoring.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., including porosity, permeability, and degree of compaction and reconsolidation; (2) Stresses and extent of..., composition, and spatial distribution; (6) Gas quantity and composition; and (7) Temperature distribution. (b...

  2. Absence of BiP co-chaperone DNAJC3 causes diabetes mellitus and multisystemic neurodegeneration.

    PubMed

    Synofzik, Matthis; Haack, Tobias B; Kopajtich, Robert; Gorza, Matteo; Rapaport, Doron; Greiner, Markus; Schönfeld, Caroline; Freiberg, Clemens; Schorr, Stefan; Holl, Reinhard W; Gonzalez, Michael A; Fritsche, Andreas; Fallier-Becker, Petra; Zimmermann, Richard; Strom, Tim M; Meitinger, Thomas; Züchner, Stephan; Schüle, Rebecca; Schöls, Ludger; Prokisch, Holger

    2014-12-04

    Diabetes mellitus and neurodegeneration are common diseases for which shared genetic factors are still only partly known. Here, we show that loss of the BiP (immunoglobulin heavy-chain binding protein) co-chaperone DNAJC3 leads to diabetes mellitus and widespread neurodegeneration. We investigated three siblings with juvenile-onset diabetes and central and peripheral neurodegeneration, including ataxia, upper-motor-neuron damage, peripheral neuropathy, hearing loss, and cerebral atrophy. Exome sequencing identified a homozygous stop mutation in DNAJC3. Screening of a diabetes database with 226,194 individuals yielded eight phenotypically similar individuals and one family carrying a homozygous DNAJC3 deletion. DNAJC3 was absent in fibroblasts from all affected subjects in both families. To delineate the phenotypic and mutational spectrum and the genetic variability of DNAJC3, we analyzed 8,603 exomes, including 506 from families affected by diabetes, ataxia, upper-motor-neuron damage, peripheral neuropathy, or hearing loss. This analysis revealed only one further loss-of-function allele in DNAJC3 and no further associations in subjects with only a subset of the features of the main phenotype. Our findings demonstrate that loss-of-function DNAJC3 mutations lead to a monogenic, recessive form of diabetes mellitus in humans. Moreover, they present a common denominator for diabetes and widespread neurodegeneration. This complements findings from mice in which knockout of Dnajc3 leads to diabetes and modifies disease in a neurodegenerative model of Marinesco-Sjögren syndrome. Copyright © 2014 The American Society of Human Genetics. Published by Elsevier Inc. All rights reserved.

  3. Persistent increase of blood lead level and suppression of δ-ALAD activity in northern bobwhite quail orally dosed with even a single 2-mm spent lead shot.

    PubMed

    Holladay, S D; Kerr, R; Holladay, J P; Meldrum, B; Williams, S M; Gogal, R M

    2012-10-01

    Birds that display grit ingestion behavior are potentially at risk of lead (Pb) poisoning from mistaken ingestion of spent Pb shot pellets. The majority of available studies designed to assess such risk have used unspent shot pellets rather than field-obtained spent shot, which is oxidized and otherwise changed by weathering. Available studies also often administered more or heavier shot pellets to a bird than it might be expected to ingest. The current study dosed northern bobwhite quail (Colinus virginianus) weighing 194.6 ± 23.1 g (female birds) and 199.3 ± 12.2 g (male birds) with one to three spent no. 9 Pb shot collected from a skeet range, with particular interest in the toxicity that may occur from ingestion of a single 2-mm, 50 mg shot. An 8 week post-dosing clinical observation period was employed, over which feed consumption, body weight, blood Pb levels, and a battery of blood physiological parameters were made. Weight loss occurred in the birds, including male birds dosed with one Pb pellet. Erythrocyte delta aminolevulinic acid dehydratase (δ-ALAD) levels were decreased for the duration of the study across exposures and to levels associated with injury in wild bird populations. Decreased ALAD was particularly severe in female birds dosed with one Pb pellet and was still 92 % decreased at 8 weeks after dosing. Together, these results suggest that inadvertent ingestion of a single no. 9 Pb shot pellet can adversely affect the health of northern bobwhite quail.

  4. A Tyrosine Residue Along with a Glutamic Acid of the Omega-Like Loop Governs the Beta-Lactamase Activity of MSMEG_4455 in Mycobacterium smegmatis.

    PubMed

    Bansal, Ankita; Kar, Debasish; Pandey, Satya Deo; Matcha, Ashok; Kumar, N Ganesh; Nathan, Soshina; Ghosh, Anindya S

    2017-06-01

    Mycobacterial beta-lactamases are involved in exerting beta-lactam resistance, though many of these proteins remain uncharacterized. Here, we have characterized MSMEG_4455 of Mycobacterium smegmatis as a beta-lactamase using molecular, biochemical and mutational techniques. To elucidate its nature in vivo and in vitro, and to predict its structure-function relationship in silico analysis is done. The MSMEG_4455 is cloned and expressed ectopically in a beta-lactamase deficient Escherichia coli mutant to establish the in vivo beta-lactamase like nature via minimum inhibitory concentration (MIC) determination. Likewise the in vivo results, purified soluble form of MSMEG_4455 showed beta-lactam hydrolysis pattern similar to group 2a penicillinase. In silico analyses of MSMEG_4455 reveal glutamic acid (E)193 and tyrosine (Y)194 of omega-like loop might have importance in strengthening hydrogen bond network around the active-site, though involvement of tyrosine is rare for beta-lactamase activity. Accordingly, these residues are mutated to alanine (A) and phenylalanine (F), respectively. The mutated proteins have partially lost their ability to exert beta-lactamase activity both in vivo and in vitro. The Y194F mutation had more prominent effect on the enzymatic activity. Therefore, we infer that Y194 is the key for beta-lactamase activity of MSMEG_4455.

  5. Worldwide Distribution of Four SNPs in X‐Ray and Repair and Cross‐Complementing Group 1 (XRCC1)

    PubMed Central

    Takeshita, Haruo; Yasuda, Toshihiro; Kimura‐Kataoka, Kaori

    2014-01-01

    Abstract Purpose X‐ray repair cross‐complementing group 1 (XRCC1) repairs single‐strand breaks in DNA. Several reports have shown the association of single nucleotide polymorphisms (SNPs) (Arg194Trp, Pro206Pro, Arg280His, Arg399Gln) in XRCC1 to diseases. Limited population data are available regarding SNPs in XRCC1, especially in African populations. In this study, genotype distributions of four SNPs in worldwide populations were examined and compared with those reported previously. Materials and Methods Four SNPs (Arg194Trp, Pro206Pro, Arg280His, Arg399Gln) in XRCC1 from genomic DNA samples of 10 populations were evaluated by using polymerase chain reaction followed by restriction fragment length polymorphism analysis. Results The frequency of the minor allele corresponding to the Trp allele of XRCC1Arg194Trp was higher in Asian populations than in African and Caucasian populations. As for XRCC1Pro206Pro, Africans showed higher minor allele frequencies than did Asian populations, except for Tamils and Sinhalese. XRCC1 Arg280His frequencies were similar among Africans and Caucasians but differed among Asian populations. Similarly, lower mutant XRCC1 Arg399Gln frequencies were observed in Africans. Conclusions This study is the first to show the existence of a certain genetic heterogeneity in the worldwide distribution of four SNPs in XRCC1. PMID:25387884

  6. Use of orthoses and orthopaedic technical devices in proximal spinal muscular atrophy. Results of survey in 194 SMA patients.

    PubMed

    Fujak, Albert; Kopschina, Carsten; Forst, Raimund; Mueller, Lutz Arne; Forst, Jürgen

    2011-01-01

    The purpose of this study is to determine the use of orthopaedic and assistive devices for Spinal muscular atrophy (SMA) patients, following a survey of 194 patients. The use of wheelchairs, corsets and orthoses was evaluated in 194 SMA patients whose mean age was 12.6 (SD 7.2, 0.7-41.1). There were 14 patients with SMA type Ib (age range 1.7-36.9), 133 with type II (age range 0.7-37.7), 42 with type IIIa (age range 3.2-41.1) and 5 with type IIIb (age range 8.0-20.0). One hundred and sixteen patients (60%) had powered and 29 patients (15%) manual wheelchairs. Nineteen patients (10%) used long leg orthoses. Ten patients (5%) used swivel walkers and 26 (13%) had standing frames. Twenty-six patients (13%) received lower leg orthoses because of foot deformities. Eight patients (4%) used night splints for the lower limbs. One hundred and fifteen patients (59%) were fitted with corsets because of progressive scoliosis. This is the first study about the provision of orthopaedic and assistive devices in a large group of SMA patients. Following the results of this survey we can optimise the strategy of providing orthoses and assistive devices for SMA patients and better adapt them to the patient's individual needs.

  7. 46 CFR 194.15-5 - Ventilation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....15-5 Ventilation. (a) Operations, reactions or experiments which produce toxic, noxious or corrosive...) Ventilation of air conditioning systems serving the chemical laboratory shall be designed so that air cannot...

  8. 49 CFR 194.107 - General response plan requirements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... management system including the functional areas of finance, logistics, operations, planning, and command... economically sensitive areas; (iii) Describe the responsibilities of the operator and of Federal, State and...

  9. Unusual Coexistence of Nickel(II) and Nickel(IV) in the Quadruple Perovskite Ba 4Ni 2Ir 2O 12 Containing Ir 2NiO 12 Mixed-Metal-Cation Trimers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ferreira, Timothy; Heald, Steve M.; Smith, Mark. D.

    In this paper, the crystal chemistry and magnetic properties of two hexagonal nickel(IV)-containing perovskites, Ba 4Ni 1.94Ir 2.06O 12 and BaNiO 3, are reported. The 12R perovskite, Ba 4Ni 1.94Ir 2.06O 12, possesses an unexpected coexistence of nickel(II) and nickel(IV). This quadruple perovskite structure contains Ir 2NiO 12 mixed-metal-cation units in which direct metal–metal bonding between nickel(IV) and iridium(V) is inferred. Finally, X-ray absorption near-edge spectroscopy and X-ray photoelectron spectroscopy measurements were conducted to confirm the simultaneous presence of nickel(II) and nickel(IV).

  10. Laminar Heat-Transfer and Pressure-Distribution Studies on a Series of Reentry Nose Shapes at a Mach Number of 19.4 in Helium

    NASA Technical Reports Server (NTRS)

    Wagner, Richard D., Jr.; Pine, W. Clint; Henderson, Arthur, Jr.

    1961-01-01

    An experimental investigation has been conducted in the 2-inch helium tunnel at the Langley Research Center at a Mach number of 19.4 to determine the pressure distributions and heat-transfer characteristics of a family of reentry nose shapes. The pressure and heat-transfer-rate distributions on the nose shapes are compared with theoretical predictions to ascertain the limitations and validity of the theories at hypersonic speeds. The experimental results were found to be adequately predicted by existing theories. Two of the nose shapes were tested with variable-length flow-separation spikes. The results obtained by previous investigators of spike-nose bodies were found to prevail at the higher Mach number of the present investigation.

  11. Unusual Coexistence of Nickel(II) and Nickel(IV) in the Quadruple Perovskite Ba 4Ni 2Ir 2O 12 Containing Ir 2NiO 12 Mixed-Metal-Cation Trimers

    DOE PAGES

    Ferreira, Timothy; Heald, Steve M.; Smith, Mark. D.; ...

    2018-03-02

    In this paper, the crystal chemistry and magnetic properties of two hexagonal nickel(IV)-containing perovskites, Ba 4Ni 1.94Ir 2.06O 12 and BaNiO 3, are reported. The 12R perovskite, Ba 4Ni 1.94Ir 2.06O 12, possesses an unexpected coexistence of nickel(II) and nickel(IV). This quadruple perovskite structure contains Ir 2NiO 12 mixed-metal-cation units in which direct metal–metal bonding between nickel(IV) and iridium(V) is inferred. Finally, X-ray absorption near-edge spectroscopy and X-ray photoelectron spectroscopy measurements were conducted to confirm the simultaneous presence of nickel(II) and nickel(IV).

  12. 46 CFR 194.10-10 - Integral magazine construction.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... spaces suitable approved incombustible thermal insulation shall be provided to prevent condensation of... to prevent condensation of moisture. Tank top manholes shall not be installed in magazines. (d) Light...

  13. 46 CFR 194.10-10 - Integral magazine construction.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... spaces suitable approved incombustible thermal insulation shall be provided to prevent condensation of... to prevent condensation of moisture. Tank top manholes shall not be installed in magazines. (d) Light...

  14. 46 CFR 194.10-10 - Integral magazine construction.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... spaces suitable approved incombustible thermal insulation shall be provided to prevent condensation of... to prevent condensation of moisture. Tank top manholes shall not be installed in magazines. (d) Light...

  15. 46 CFR 194.10-10 - Integral magazine construction.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... spaces suitable approved incombustible thermal insulation shall be provided to prevent condensation of... to prevent condensation of moisture. Tank top manholes shall not be installed in magazines. (d) Light...

  16. 46 CFR 194.15-19 - Electrical.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....15-19 Electrical. (a) All electrical equipment located within 18 inches of the deck of the chemical laboratory shall be in accordance with the applicable requirements of Subchapter J (Electrical Engineering...

  17. 46 CFR 194.15-19 - Electrical.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....15-19 Electrical. (a) All electrical equipment located within 18 inches of the deck of the chemical laboratory shall be in accordance with the applicable requirements of Subchapter J (Electrical Engineering...

  18. 46 CFR 194.15-19 - Electrical.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....15-19 Electrical. (a) All electrical equipment located within 18 inches of the deck of the chemical laboratory shall be in accordance with the applicable requirements of Subchapter J (Electrical Engineering...

  19. 46 CFR 194.15-19 - Electrical.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....15-19 Electrical. (a) All electrical equipment located within 18 inches of the deck of the chemical laboratory shall be in accordance with the applicable requirements of Subchapter J (Electrical Engineering...

  20. 46 CFR 194.15-19 - Electrical.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ....15-19 Electrical. (a) All electrical equipment located within 18 inches of the deck of the chemical laboratory shall be in accordance with the applicable requirements of Subchapter J (Electrical Engineering...

  1. 21 CFR 155.194 - Catsup.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...). (ii) The liquid derived from mature tomatoes of the red or reddish varieties Lycopersicum esculentum P... individual serving-size packages containing 56.7 grams (2 ounces) or less. (2) Determine compliance as...

  2. 21 CFR 155.194 - Catsup.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...). (ii) The liquid derived from mature tomatoes of the red or reddish varieties Lycopersicum esculentum P... individual serving-size packages containing 56.7 grams (2 ounces) or less. (2) Determine compliance as...

  3. 21 CFR 155.194 - Catsup.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...). (ii) The liquid derived from mature tomatoes of the red or reddish varieties Lycopersicum esculentum P... individual serving-size packages containing 56.7 grams (2 ounces) or less. (2) Determine compliance as...

  4. 21 CFR 155.194 - Catsup.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...). (ii) The liquid derived from mature tomatoes of the red or reddish varieties Lycopersicum esculentum P... individual serving-size packages containing 56.7 grams (2 ounces) or less. (2) Determine compliance as...

  5. Making good better: implementing a standardized handoff in pediatric transport.

    PubMed

    Weingart, Caroline; Herstich, Tracey; Baker, Pam; Garrett, M Lynne; Bird, Michael; Billock, James; Schwartz, Hamilton P; Bigham, Michael T

    2013-01-01

    Failures in communication lead to adverse events in healthcare. Handoffs, defined as the transfer of information, responsibility, and authority from one provider to another, have been identified as a cause of communication failure compromising patient safety. Locally, there was dissatisfaction among caregivers working on the general care and intensive care units regarding the quality of information received from the pediatric transport team for transferred patients. Using the Model for Improvement, a quality improvement team was engaged to lead this improvement effort. The team developed a standardized and scripted transport handoff process that incorporated parental input. The primary measure was provider satisfaction (reported as overall handoff score, OHS). Secondary outcomes included the use of components outlined by the Joint Commission's guidelines for safe handoff. Data were collected using a Likert-style survey and collated using Microsoft Excel. Baseline measures of OHS were 81.5 ± 19.4 (mean±SD) with an interval analysis showing no improvement (81.6±17.4, P=0.99). Further modifications were made to both education and process with an improved OHS (88.8±11.1, P<0.05). Certain specific handoff components showed the greatest improvement according to caregivers. This practical, low-cost quality-improvement project may help others improve handoff communication and provide safe, high-quality care. Copyright © 2013 Air Medical Journal Associates. Published by Elsevier Inc. All rights reserved.

  6. The common causes leading to pancytopenia in patients presenting to tertiary care hospital

    PubMed Central

    Das Makheja, Kirpal; Kumar Maheshwari, Bharat; Arain, Shafique; Kumar, Suneel; Kumari, Sangeeta; Vikash

    2013-01-01

    Objective: The objective of this study was to determine the frequency of common causes leading to Pancytopenia in patients presenting to tertiary care hospital at Karachi. Methods: A total of 62 patients with the diagnosis of Pancytopenia of more than one week duration were enrolled in the study. All patients underwent a detailed medical history and full physical examination followed by blood sampling for the investigations i.e. complete blood count with peripheral film, erythrocyte sedimentation rate (ESR), Malarial parasites (MP), liver function test, Renal function tests, PT and viral profile (HBsAg, Anti-HCV), Ultrasonography of abdomen. All patients underwent bone marrow aspiration and trephine biopsy for reporting and interpretation. Duration of study was six months, from May 2010 to November 2010. Results: The average age of the patients was 37.76 ± 16.38years. Out of 62 patients, 36 (58%) were male and 26 (42%) were female. Megaloblastic anemia was the commonest cause that was observed in 41.9% cases followed by acute myeloid leukemia 27.4%, aplastic anemia 19.4% and erythroid hyperplasia 11.3%. Conclusion : This study concluded that most common cause of pancytopenia is Megaloblastic anemia, followed by acute myeloid leukemia and aplastic anemia. Bone marrow examination is a single useful investigation which reveals the underlying cause in patients with pancytopenia. PMID:24353701

  7. Search for gluino mediated bottom- and top-squark production in multijet final states in pp collisions at 8 TeV

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Chatrchyan, S.; Khachatryan, V.; Sirunyan, A. M.

    2013-10-01

    A search for supersymmetry is presented based on events with large missing transverse energy, no isolated electron or muon, and at least three jets with one or more identified as a bottom-quark jet. A simultaneous examination is performed of the numbers of events in exclusive bins of the scalar sum of jet transverse momentum values, missing transverse energy, and bottom-quark jet multiplicity. The sample, corresponding to an integrated luminosity of 19.4 inverse femtobarns, consists of proton-proton collision data recorded at a center-of-mass energy of 8 TeV with the CMS detector at the LHC in 2012. The observed numbers of eventsmore » are found to be consistent with the standard model expectation, which is evaluated with control samples in data. The results are interpreted in the context of two simplified supersymmetric scenarios in which gluino pair production is followed by the decay of each gluino to an undetected lightest supersymmetric particle and either a bottom or top quark-antiquark pair, characteristic of gluino mediated bottom- or top-squark production. Using the production cross section calculated to next-to-leading-order plus next-to-leading-logarithm accuracy, and in the limit of a massless lightest supersymmetric particle, we exclude gluinos with masses below 1170 GeV and 1020 GeV for the two scenarios, respectively.« less

  8. 46 CFR 194.15-3 - Responsibility.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....15-3 Responsibility. (a) With the knowledge and approval of the master, the senior member of the... safeguards against hazardous undertakings. (3) Educate personnel working in the laboratory spaces to be alert...

  9. 46 CFR 194.15-3 - Responsibility.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....15-3 Responsibility. (a) With the knowledge and approval of the master, the senior member of the... safeguards against hazardous undertakings. (3) Educate personnel working in the laboratory spaces to be alert...

  10. Effect of Family Structure on Marital Attitudes of Adolescents.

    ERIC Educational Resources Information Center

    Ganong, Lawrence; And Others

    1981-01-01

    Assesses the effect of exposure to different family structures (single parent families, reconstituted families, intact families) on the marital socialization of 127 males and 194 female adolescents. (Author/CM)

  11. National Blue Alert Act of 2013

    THOMAS, 113th Congress

    Sen. Cardin, Benjamin L. [D-MD

    2013-02-14

    Senate - 09/19/2013 Placed on Senate Legislative Calendar under General Orders. Calendar No. 194. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  12. 40 CFR 194.43 - Passive institutional controls.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... the location of such information; and (v) The results of tests, experiments, and other analyses... other tests, experiments, or analyses pertinent to the containment of waste in the disposal system, or...

  13. 40 CFR 194.43 - Passive institutional controls.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the location of such information; and (v) The results of tests, experiments, and other analyses... other tests, experiments, or analyses pertinent to the containment of waste in the disposal system, or...

  14. 40 CFR 194.43 - Passive institutional controls.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... the location of such information; and (v) The results of tests, experiments, and other analyses... other tests, experiments, or analyses pertinent to the containment of waste in the disposal system, or...

  15. 46 CFR 194.05-7 - Explosives-Detail requirements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... shall be constructed of incombustible materials; awnings and tarpaulins shall be fire-resistant and/or flame proof fabric. (3) Items shall be properly secured by using existing vessel structures such as...

  16. 42 CFR 413.194 - Appeals.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... facility that disputes the amount of its allowable Medicare bad debts reimbursed by CMS under § 413.178 may... prior to seeking judicial review of a dispute regarding the amount of allowable Medicare bad debts. (b...

  17. 15 CFR 922.194 - Certification of preexisting leases, licenses, permits, approvals, other authorizations, or...

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... MANAGEMENT NATIONAL MARINE SANCTUARY PROGRAM REGULATIONS Thunder Bay National Marine Sanctuary and Underwater..., Office of Ocean and Coastal Resource Management; ATTN: Sanctuary Manager, Thunder Bay National Marine...

  18. 15 CFR 922.194 - Certification of preexisting leases, licenses, permits, approvals, other authorizations, or...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... MANAGEMENT NATIONAL MARINE SANCTUARY PROGRAM REGULATIONS Thunder Bay National Marine Sanctuary and Underwater..., Office of Ocean and Coastal Resource Management; ATTN: Sanctuary Manager, Thunder Bay National Marine...

  19. 15 CFR 922.194 - Certification of preexisting leases, licenses, permits, approvals, other authorizations, or...

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... MANAGEMENT NATIONAL MARINE SANCTUARY PROGRAM REGULATIONS Thunder Bay National Marine Sanctuary and Underwater..., Office of Ocean and Coastal Resource Management; ATTN: Sanctuary Manager, Thunder Bay National Marine...

  20. 15 CFR 922.194 - Certification of preexisting leases, licenses, permits, approvals, other authorizations, or...

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... MANAGEMENT NATIONAL MARINE SANCTUARY PROGRAM REGULATIONS Thunder Bay National Marine Sanctuary and Underwater..., Office of Ocean and Coastal Resource Management; ATTN: Sanctuary Manager, Thunder Bay National Marine...

  1. 15 CFR 922.194 - Certification of preexisting leases, licenses, permits, approvals, other authorizations, or...

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... MANAGEMENT NATIONAL MARINE SANCTUARY PROGRAM REGULATIONS Thunder Bay National Marine Sanctuary and Underwater..., Office of Ocean and Coastal Resource Management; ATTN: Sanctuary Manager, Thunder Bay National Marine...

  2. 49 CFR 194.5 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... wave height as specified in 33 CFR Part 154, Appendix C, Table 1, and currents within the areas in which those systems or equipment are intended to function. Barrel means 42 United States gallons (159...

  3. Is Current US Counterinsurgency Doctrine Applicable to Lebanese Hizballah and the Taliban?

    DTIC Science & Technology

    2010-06-11

    credibility of the Afghan government.180 180Griff White, “Taliban Shadow Officials Offer Concrete ...194International Crisis Group, “Egypts Muslim Brothers: Integration or Conflagration ?” Middle East

  4. 49 CFR 194.105 - Worst case discharge.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...: Prevention measure Standard Credit(percent) Secondary containment >100% NFPA 30 50 Built/repaired to API standards API STD 620/650/653 10 Overfill protection standards API RP 2350 5 Testing/cathodic protection API...

  5. 49 CFR 194.105 - Worst case discharge.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...: Prevention measure Standard Credit(percent) Secondary containment > 100% NFPA 30 50 Built/repaired to API standards API STD 620/650/653 10 Overfill protection standards API RP 2350 5 Testing/cathodic protection API...

  6. 49 CFR 194.105 - Worst case discharge.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...: Prevention measure Standard Credit(percent) Secondary containment >100% NFPA 30 50 Built/repaired to API standards API STD 620/650/653 10 Overfill protection standards API RP 2350 5 Testing/cathodic protection API...

  7. 49 CFR 194.105 - Worst case discharge.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...: Prevention measure Standard Credit(percent) Secondary containment > 100% NFPA 30 50 Built/repaired to API standards API STD 620/650/653 10 Overfill protection standards API RP 2350 5 Testing/cathodic protection API...

  8. 49 CFR 194.105 - Worst case discharge.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...: Prevention measure Standard Credit(percent) Secondary containment > 100% NFPA 30 50 Built/repaired to API standards API STD 620/650/653 10 Overfill protection standards API RP 2350 5 Testing/cathodic protection API...

  9. Proposed standard-weight (Ws) equation and length-categorization standards for brown trout (Salmo trutta) in lentic habitats

    USGS Publications Warehouse

    Hyatt, M.W.; Hubert, W.A.

    2001-01-01

    We developed a standard-weight (Ws) equation for brown trout (Salmo trutta) in lentic habitats by applying the regression-line-percentile technique to samples from 49 populations in North America. The proposed Ws equation is log10 Ws = -5.422 + 3.194 log10 TL, when Ws is in grams and TL is total length in millimeters. The English-unit equivalent is log10 Ws = -3.592 + 3.194 log10 TL, when Ws is in pounds and TL is total length in inches. The equation is applicable for fish of 140-750 mm TL. Proposed length-category standards to evaluate fish within populations are: stock, 200 mm (8 in); quality, 300 mm (12 in); preferred, 400 mm (16 in); memorable, 500 mm (20 in); and trophy, 600 mm (24 in).

  10. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Bernards, C.; Heinze, S.; Jolie, J.

    We present the results of a {gamma}{gamma} angular correlation experiment investigating the nucleus {sup 196}Hg and compare these with a theoretical description of {sup 196}Hg within the U{sub {nu}}(6/12) x U{sub {pi}}(6/4) extended supersymmetry. To populate excited {sup 196}Hg states, we used the Cologne FN Tandem accelerator inducing the reaction {sup 194}Pt({alpha},2n){sup 196}Hg and analyzed the {gamma} decays of levels up to an excitation energy of 2.4 MeV with the HORUS cube spectrometer. The new results on this mercury isotope allow a comparison between the experimental data and the supersymmetrical predictions and show good agreement. This way we can addmore » {sup 196}Hg as a fifth supermultiplet member to the so-called magical quartet consisting of {sup 194,195}Pt and {sup 195,196}Au.« less

  11. Extension of activation cross section data of long lived products in deuteron induced nuclear reactions on platinum up to 50 MeV

    NASA Astrophysics Data System (ADS)

    Ditrói, F.; Tárkányi, F.; Takács, S.; Hermanne, A.

    2017-06-01

    In the frame of a systematical study of light ion induced nuclear reactions on platinum, activation cross sections for deuteron induced reactions were investigated. Excitation functions were measured in the 20.8-49.2 MeV energy range for the natPt(d,xn)191,192,193,194,195,196m2,196g,198g,199Au, natPt(d,x)188,189,191,195m,197m,197gPt and natPt(d,x)189,190,192,194m2Ir reactions by using the stacked foil irradiation technique. The experimental results are compared with previous results from the literature and with the theoretical predictions in the TENDL-2014 and TENDL-2015 libraries. The applicability of the produced radio-tracers for wear measurements has been presented.

  12. Injury and mechanism of recombinant E. coli expressing STa on piglets colon.

    PubMed

    Lv, Yang; Li, Xueni; Zhang, Lin; Shi, Yutao; DU, Linxiao; Ding, Binying; Hou, Yongqing; Gong, Joshua; Wu, Tao

    2018-02-09

    Enterotoxigenic Escherichia coli (ETEC) is primary pathogenic bacteria of piglet diarrhea, over two thirds of piglets diarrhea caused by ETEC are resulted from STa-producing ETEC strains. This experiment was conducted to construct the recombinant E. coli expressing STa and study the injury and mechanism of recombinant E. coli expressing STa on 7 days old piglets colon. Twenty-four 7 days old piglets were allotted to four treatments: control group, STa group (2 × 10 9 CFU E. coli LMG194-STa), LMG194 group (2 × 10 9 CFU E. coli LMG194) and K88 group (2 × 10 9 CFU E. coli K88). The result showed that E. coli infection significantly increased diarrhea rates; changed DAO activity in plasma and colon; damaged colonic mucosal morphology including crypt depth, number of globet cells, density of lymphocytes and lamina propria cell density; substantially reduced antioxidant capacity by altering activities of GSH-Px, SOD, and TNOS and productions of MDA and H 2 O 2 ; obviously decreased AQP3, AQP4 and KCNJ13 protein expression levels; substantially altered the gene expression levels of inflammatory cytokines. Conclusively, STa group had the biggest effect on these indices in four treatment groups. These results suggested that the recombinant strain expressed STa can induce piglets diarrhea and colonic morphological and funtional damage by altering expression of proteins connect to transportation function and genes associated with intestinal injury and inflammatory cytokines.

  13. Injury and mechanism of recombinant E. coli expressing STa on piglets colon

    PubMed Central

    LV, Yang; LI, Xueni; ZHANG, Lin; SHI, Yutao; DU, Linxiao; DING, Binying; HOU, Yongqing; GONG, Joshua; WU, Tao

    2017-01-01

    Enterotoxigenic Escherichia coli (ETEC) is primary pathogenic bacteria of piglet diarrhea, over two thirds of piglets diarrhea caused by ETEC are resulted from STa-producing ETEC strains. This experiment was conducted to construct the recombinant E. coli expressing STa and study the injury and mechanism of recombinant E. coli expressing STa on 7 days old piglets colon. Twenty-four 7 days old piglets were allotted to four treatments: control group, STa group (2 × 109 CFU E. coli LMG194-STa), LMG194 group (2 × 109 CFU E. coli LMG194) and K88 group (2 × 109 CFU E. coli K88). The result showed that E. coli infection significantly increased diarrhea rates; changed DAO activity in plasma and colon; damaged colonic mucosal morphology including crypt depth, number of globet cells, density of lymphocytes and lamina propria cell density; substantially reduced antioxidant capacity by altering activities of GSH-Px, SOD, and TNOS and productions of MDA and H2O2; obviously decreased AQP3, AQP4 and KCNJ13 protein expression levels; substantially altered the gene expression levels of inflammatory cytokines. Conclusively, STa group had the biggest effect on these indices in four treatment groups. These results suggested that the recombinant strain expressed STa can induce piglets diarrhea and colonic morphological and funtional damage by altering expression of proteins connect to transportation function and genes associated with intestinal injury and inflammatory cytokines. PMID:29187713

  14. Operation of a New Half-Bridge Gate Driver for Enhancement - Mode GaN FETs, Type LM5113, Over a Wide Temperature Range

    NASA Technical Reports Server (NTRS)

    Patterson, Richard; Hammoud, Ahmad

    2011-01-01

    A new commercial-off-the-shelf (COTS) gate driver designed to drive both the high-side and the low-side enhancement-mode GaN FETs, National Semiconductor's type LM5113, was evaluated for operation at temperatures beyond its recommended specified limits of -40 C to +125 C. The effects of limited thermal cycling under the extended test temperature, which ranged from -194 C to +150 C, on the operation of this chip as well as restart capability at the extreme cryogenic and hot temperatures were also investigated. The driver circuit was able to maintain good operation throughout the entire test regime between -194 C and +150 C without undergoing any major changes in its outputs signals and characteristics. The limited thermal cycling performed on the device also had no effect on its performance, and the driver chip was able to successfully restart at each of the extreme temperatures of -194 C and +150 C. The plastic packaging of this device was also not affected by either the short extreme temperature exposure or the limited thermal cycling. These preliminary results indicate that this new commercial-off-the-shelf (COTS) halfbridge eGaN FET driver integrated circuit has the potential for use in space exploration missions under extreme temperature environments. Further testing is planned under long-term cycling to assess the reliability of these parts and to determine their suitability for extended use in the harsh environments of space.

  15. 49 CFR 572.194 - Shoulder.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., while the midsagittal plane of the dummy is in vertical orientation. (4) Push the dummy at the knees and... back until the back of the upper torso is in contact with the seat back. (5) While maintaining the...

  16. 40 CFR 300.185 - Nongovernmental participation.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...; 40 CFR part 112; and 49 CFR parts 171 and 194. (b) The technical and scientific information generated... § 300.150 regarding worker health and safety. ACPs should provide for the direction of volunteers by the...

  17. 40 CFR 300.185 - Nongovernmental participation.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...; 40 CFR part 112; and 49 CFR parts 171 and 194. (b) The technical and scientific information generated... § 300.150 regarding worker health and safety. ACPs should provide for the direction of volunteers by the...

  18. 40 CFR 300.185 - Nongovernmental participation.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...; 40 CFR part 112; and 49 CFR parts 171 and 194. (b) The technical and scientific information generated... § 300.150 regarding worker health and safety. ACPs should provide for the direction of volunteers by the...

  19. Materials Data on Ca (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2015-02-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  20. 40 CFR 194.54 - Scope of compliance assessments.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... processes and events that may occur over the regulatory time frame; (2) Identifies the processes, events, or... effects on the disposal system of: (1) Existing boreholes in the vicinity of the disposal system, with...

  1. Materials Data on In (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-02-11

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  2. Materials Data on SbIr (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2015-02-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  3. Materials Data on GaN (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-09-15

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  4. Materials Data on CaPAu (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2015-02-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  5. Materials Data on VO2 (SG:194) by Materials Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kristin Persson

    2014-09-30

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  6. Materials Data on CuS (SG:194) by Materials Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kristin Persson

    2016-04-22

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  7. Materials Data on Mg (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-02-11

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  8. Materials Data on K (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-02-05

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  9. Materials Data on N2 (SG:194) by Materials Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kristin Persson

    2014-11-02

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  10. Materials Data on ZrC (SG:194) by Materials Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kristin Persson

    2016-12-23

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  11. Materials Data on Y (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2015-02-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  12. 49 CFR Appendix B to Part 194 - High Volume Areas

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Glaise, AR. Wisconsin River Wisconsin Rapids, WI. Yukon River Fairbanks, AK. Other Navigable Waters Arthur Kill Channel, NY Cook Inlet, AK Freeport, TX Los Angeles/Long Beach Harbor, CA Port Lavaca, TX San...

  13. 49 CFR Appendix B to Part 194 - High Volume Areas

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Glaise, AR. Wisconsin River Wisconsin Rapids, WI. Yukon River Fairbanks, AK. Other Navigable Waters Arthur Kill Channel, NY Cook Inlet, AK Freeport, TX Los Angeles/Long Beach Harbor, CA Port Lavaca, TX San...

  14. 49 CFR Appendix B to Part 194 - High Volume Areas

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Glaise, AR. Wisconsin River Wisconsin Rapids, WI. Yukon River Fairbanks, AK. Other Navigable Waters Arthur Kill Channel, NY Cook Inlet, AK Freeport, TX Los Angeles/Long Beach Harbor, CA Port Lavaca, TX San...

  15. 49 CFR Appendix B to Part 194 - High Volume Areas

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Glaise, AR. Wisconsin River Wisconsin Rapids, WI. Yukon River Fairbanks, AK. Other Navigable Waters Arthur Kill Channel, NY Cook Inlet, AK Freeport, TX Los Angeles/Long Beach Harbor, CA Port Lavaca, TX San...

  16. Materials Data on C (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2014-07-09

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  17. Materials Data on C (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-04-20

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  18. Materials Data on Tb (SG:194) by Materials Project

    DOE Data Explorer

    Kristin Persson

    2016-02-10

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  19. Materials Data on ReN (SG:194) by Materials Project

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kristin Persson

    2016-09-03

    Computed materials data using density functional theory calculations. These calculations determine the electronic structure of bulk materials by solving approximations to the Schrodinger equation. For more information, see https://materialsproject.org/docs/calculations

  20. 40 CFR 194.5 - Publications incorporated by reference.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... PROTECTION PROGRAMS CRITERIA FOR THE CERTIFICATION AND RE-CERTIFICATION OF THE WASTE ISOLATION PILOT PLANT'S... availability of this material at NARA, call 202-741-6030, or go to: http://www.archives.gov/federal_register...

Some links on this page may take you to non-federal websites. Their policies may differ from this site.
Top