Ubiquitin conjugation by the N-end rule pathway and mRNAs for its components increase in muscles of diabetic rats

PubMed Central

Lecker, Stewart H.; Solomon, Vered; Price, S. Russ; Kwon, Yong Tae; Mitch, William E.; Goldberg, Alfred L.

1999-01-01

Insulin deficiency (e.g., in acute diabetes or fasting) is associated with enhanced protein breakdown in skeletal muscle leading to muscle wasting. Because recent studies have suggested that this increased proteolysis is due to activation of the ubiquitin-proteasome (Ub-proteasome) pathway, we investigated whether diabetes is associated with an increased rate of Ub conjugation to muscle protein. Muscle extracts from streptozotocin-induced insulin-deficient rats contained greater amounts of Ub-conjugated proteins than extracts from control animals and also 40–50% greater rates of conjugation of 125I-Ub to endogenous muscle proteins. This enhanced Ub-conjugation occurred mainly through the N-end rule pathway that involves E214k and E3α. A specific substrate of this pathway, α-lactalbumin, was ubiquitinated faster in the diabetic extracts, and a dominant negative form of E214k inhibited this increase in ubiquitination rates. Both E214k and E3α were shown to be rate-limiting for Ub conjugation because adding small amounts of either to extracts stimulated Ub conjugation. Furthermore, mRNA for E214k and E3α (but not E1) were elevated 2-fold in muscles from diabetic rats, although no significant increase in E214k and E3α content could be detected by immunoblot or activity assays. The simplest interpretation of these results is that small increases in both E214k and E3α in muscles of insulin-deficient animals together accelerate Ub conjugation and protein degradation by the N-end rule pathway, the same pathway activated in cancer cachexia, sepsis, and hyperthyroidism. J. Clin. Invest. 104:1411–1420 (1999). PMID:10562303

Ubiquitin conjugation by the N-end rule pathway and mRNAs for its components increase in muscles of diabetic rats.

PubMed

Lecker, S H; Solomon, V; Price, S R; Kwon, Y T; Mitch, W E; Goldberg, A L

1999-11-01

Insulin deficiency (e.g., in acute diabetes or fasting) is associated with enhanced protein breakdown in skeletal muscle leading to muscle wasting. Because recent studies have suggested that this increased proteolysis is due to activation of the ubiquitin-proteasome (Ub-proteasome) pathway, we investigated whether diabetes is associated with an increased rate of Ub conjugation to muscle protein. Muscle extracts from streptozotocin-induced insulin-deficient rats contained greater amounts of Ub-conjugated proteins than extracts from control animals and also 40-50% greater rates of conjugation of (125)I-Ub to endogenous muscle proteins. This enhanced Ub-conjugation occurred mainly through the N-end rule pathway that involves E2(14k) and E3alpha. A specific substrate of this pathway, alpha-lactalbumin, was ubiquitinated faster in the diabetic extracts, and a dominant negative form of E2(14k) inhibited this increase in ubiquitination rates. Both E2(14k) and E3alpha were shown to be rate-limiting for Ub conjugation because adding small amounts of either to extracts stimulated Ub conjugation. Furthermore, mRNA for E2(14k) and E3alpha (but not E1) were elevated 2-fold in muscles from diabetic rats, although no significant increase in E2(14k) and E3alpha content could be detected by immunoblot or activity assays. The simplest interpretation of these results is that small increases in both E2(14k) and E3alpha in muscles of insulin-deficient animals together accelerate Ub conjugation and protein degradation by the N-end rule pathway, the same pathway activated in cancer cachexia, sepsis, and hyperthyroidism.

Ubiquitin conjugation by the N-end rule pathway and mRNAs for its components increase in muscles of diabetic rats

NASA Technical Reports Server (NTRS)

Lecker, S. H.; Solomon, V.; Price, S. R.; Kwon, Y. T.; Mitch, W. E.; Goldberg, A. L.

1999-01-01

Insulin deficiency (e.g., in acute diabetes or fasting) is associated with enhanced protein breakdown in skeletal muscle leading to muscle wasting. Because recent studies have suggested that this increased proteolysis is due to activation of the ubiquitin-proteasome (Ub-proteasome) pathway, we investigated whether diabetes is associated with an increased rate of Ub conjugation to muscle protein. Muscle extracts from streptozotocin-induced insulin-deficient rats contained greater amounts of Ub-conjugated proteins than extracts from control animals and also 40-50% greater rates of conjugation of (125)I-Ub to endogenous muscle proteins. This enhanced Ub-conjugation occurred mainly through the N-end rule pathway that involves E2(14k) and E3alpha. A specific substrate of this pathway, alpha-lactalbumin, was ubiquitinated faster in the diabetic extracts, and a dominant negative form of E2(14k) inhibited this increase in ubiquitination rates. Both E2(14k) and E3alpha were shown to be rate-limiting for Ub conjugation because adding small amounts of either to extracts stimulated Ub conjugation. Furthermore, mRNA for E2(14k) and E3alpha (but not E1) were elevated 2-fold in muscles from diabetic rats, although no significant increase in E2(14k) and E3alpha content could be detected by immunoblot or activity assays. The simplest interpretation of these results is that small increases in both E2(14k) and E3alpha in muscles of insulin-deficient animals together accelerate Ub conjugation and protein degradation by the N-end rule pathway, the same pathway activated in cancer cachexia, sepsis, and hyperthyroidism.

Rotigotine polyoxazoline conjugate SER-214 provides robust and sustained antiparkinsonian benefit.

PubMed

Eskow Jaunarajs, Karen L; Standaert, David G; Viegas, Tacey X; Bentley, Michael D; Fang, Zhihao; Dizman, Bekir; Yoon, Kunsang; Weimer, Rebecca; Ravenscroft, Paula; Johnston, Tom H; Hill, Michael P; Brotchie, Jonathan M; Moreadith, Randall W

2013-10-01

Currently available dopaminergic drugs such as levodopa and dopamine (DA) receptor agonists impart considerable improvement in Parkinson's disease (PD) motor symptoms but often lead to significant motor complications including "wearing-off" and dyskinesia. Such complications are believed to stem from the pulsatile nature of dopaminergic stimulation with these agents. Continuous dopaminergic drug delivery using polyoxazoline (POZ) polymer conjugation may improve motor symptoms, while avoiding development of side effects. The purposes of the current study are to characterize the in vitro and in vivo pharmacokinetics of POZ conjugation of a U.S. Food and Drug Administration (FDA)-approved DA agonist, rotigotine, and to evaluate their effects in an established rat model of PD. After determination of release profiles of several POZ-conjugated constructs ("fast": SER-212; "moderate": SER-213; and "slow": SER-214) using in vitro hydrolysis, normal male Sprague-Dawley rats were used for determination of the pharmacokinetic profile of both acute and chronic exposure. Finally, a separate group of rats was rendered hemiparkinsonian using intracranial 6-hydroxydopamine (6-OHDA) infusions, treated acutely with POZ-rotigotine, and assessed for rotational behavior and antiparkinsonian benefit using the cylinder test. POZ-rotigotine formulations SER-213 and SER-214 led to substantial pharmacokinetic improvement compared to unconjugated rotigotine. In addition, SER-214 led to antiparkinsonian effects in DA-lesioned rats that persisted up to 5 days posttreatment. Repeated weekly dose administration of SER-214 to normal rats for up to 12 weeks demonstrated highly reproducible pharmacokinetic profiles. The continuous dopaminergic stimulation profile afforded by SER-214 could represent a significant advance in the treatment of PD, with potential to be a viable, once-per-week therapy for PD patients. © 2013 Movement Disorder Society.

Resistance Training Regulates Cardiac Function through Modulation of miRNA-214

PubMed Central

Melo, Stéphano Freitas Soares; Barauna, Valério Garrone; Júnior, Miguel Araújo Carneiro; Bozi, Luiz Henrique Marchesi; Drummond, Lucas Rios; Natali, Antônio José; de Oliveira, Edilamar Menezes

2015-01-01

Aims: To determine the effects of resistance training (RT) on the expression of microRNA (miRNA)-214 and its target in sarcoplasmic reticulum Ca2+-ATPase (SERCA2a), and on the morphological and mechanical properties of isolated left ventricular myocytes. Main methods: Male Wistar rats were divided into two groups (n = 7/group): Control (CO) or trained (TR). The exercise-training protocol consisted of: 4 × 12 bouts, 5×/week during 8 weeks, with 80% of one repetition maximum. Key findings: RT increased the left ventricular myocyte width by 15% and volume by 12%, compared with control animals (p < 0.05). The time to half relaxation and time to peak were 8.4% and 4.4% lower, respectively, in cells from TR group as compared to CO group (p < 0.05). RT decreased miRNA-214 level by 18.5% while its target SERCA2a expression were 18.5% higher (p < 0.05). Significance: Our findings showed that RT increases single left ventricular myocyte dimensions and also leads to faster cell contraction and relaxation. These mechanical adaptations may be related to the augmented expression of SERCA2a which, in turn, may be associated with the epigenetic modification of decreased miRNA-214 expression. PMID:25822872

Mutation-Induced Changes in the Protein Environment and Site Energies in the (M)L214G Mutant of the Rhodobacter sphaeroides Bacterial Reaction Center.

PubMed

Jankowiak, Ryszard; Rancova, Olga; Chen, Jinhai; Kell, Adam; Saer, Rafael G; Beatty, J Thomas; Abramavicius, Darius

2016-08-18

This work focuses on the low-temperature (5 K) photochemical (transient) hole-burned (HB) spectra within the P870 absorption band, and their theoretical analysis, for the (M)L214G mutant of the photosynthetic Rhodobacter sphaeroides bacterial reaction center (bRC). To provide insight into system-bath interactions of the bacteriochlorophyll a (BChl a) special pair, i.e., P870, in the mutated bRC, the optical line shape function for the P870 band is calculated numerically. On the basis of the modeling studies, we demonstrate that (M)L214G mutation leads to a heterogeneous population of bRCs with modified (increased) total electron-phonon coupling strength of the special pair BChl a and larger inhomogeneous broadening. Specifically, we show that after mutation in the (M)L214G bRC a large fraction (∼50%) of the bacteriopheophytin (HA) chromophores shifts red and the 800 nm absorption band broadens, while the remaining fraction of HA cofactors retains nearly the same site energy as HA in the wild-type bRC. Modeling using these two subpopulations allowed for fits of the absorption and nonresonant (transient) HB spectra of the mutant bRC in the charge neutral, oxidized, and charge-separated states using the Frenkel exciton Hamiltonian, providing new insight into the mutant's complex electronic structure. Although the average (M)L214G mutant quantum efficiency of P(+)QA(-) state formation seems to be altered in comparison with the wild-type bRC, the average electron transfer time (measured via resonant transient HB spectra within the P870 band) was not affected. Thus, mutation in the vicinity of the electron acceptor (HA) does not tune the charge separation dynamics. Finally, quenching of the (M)L214G mutant excited states by P(+) is addressed by persistent HB spectra burned within the B band in chemically oxidized samples.

42 CFR 57.214 - Repayment of loans made after November 17, 1971, for failure to complete a program of study.

Code of Federal Regulations, 2011 CFR

2011-10-01

... have failed to complete the course of study leading to the first professional degree for which an..., adjusted annually for changes in the Consumer Price Index, and adjusted by the Secretary for use in this...

42 CFR 57.214 - Repayment of loans made after November 17, 1971, for failure to complete a program of study.

Code of Federal Regulations, 2010 CFR

2010-10-01

... have failed to complete the course of study leading to the first professional degree for which an..., adjusted annually for changes in the Consumer Price Index, and adjusted by the Secretary for use in this...

40 CFR 464.25 - Pretreatment standards for existing sources.

Code of Federal Regulations, 2014 CFR

2014-07-01

....84 2.86 Zinc (T) 8.37 3.19 TTO 25.4 8.29 Oil and grease for alternate monitoring) 330 110 (f) Melting....214 Lead (T) 0.27 0.132 Zinc (T) 0.387 0.148 TTO 0.428 0.14 Oil and grease (for alternate monitoring... Copper (T) 0.0307 0.0168 Lead (T) 0.0211 0.0104 Zinc (T) 0.0303 0.0116 TTO 0.0335 0.0109 Oil and grease...

40 CFR 464.25 - Pretreatment standards for existing sources.

Code of Federal Regulations, 2013 CFR

2013-07-01

....84 2.86 Zinc (T) 8.37 3.19 TTO 25.4 8.29 Oil and grease for alternate monitoring) 330 110 (f) Melting....214 Lead (T) 0.27 0.132 Zinc (T) 0.387 0.148 TTO 0.428 0.14 Oil and grease (for alternate monitoring... Copper (T) 0.0307 0.0168 Lead (T) 0.0211 0.0104 Zinc (T) 0.0303 0.0116 TTO 0.0335 0.0109 Oil and grease...

40 CFR 464.25 - Pretreatment standards for existing sources.

Code of Federal Regulations, 2012 CFR

2012-07-01

....84 2.86 Zinc (T) 8.37 3.19 TTO 25.4 8.29 Oil and grease for alternate monitoring) 330 110 (f) Melting....214 Lead (T) 0.27 0.132 Zinc (T) 0.387 0.148 TTO 0.428 0.14 Oil and grease (for alternate monitoring... Copper (T) 0.0307 0.0168 Lead (T) 0.0211 0.0104 Zinc (T) 0.0303 0.0116 TTO 0.0335 0.0109 Oil and grease...

Apples for Teachers Pay Off.

ERIC Educational Resources Information Center

Geller, Irving, Ed.

1983-01-01

Reviews current trends in the educational market for microcomputers and software. As of June 1982, about 214,000 microcomputers were installed in schools, with Apple Computer (followed by Radio Shack and others) leading the field. A new federal program virtually eliminating how schools use funds may benefit computer assisted instruction. (JN)

Thinking in Categories or along a Continuum: Consequences for Children's Social Judgments

ERIC Educational Resources Information Center

Master, Allison; Markman, Ellen M.; Dweck, Carol S.

2012-01-01

Can young children, forming expectations about the social world, capture differences among people without falling into the pitfalls of categorization? Categorization often leads to exaggerating differences between groups and minimizing differences within groups, resulting in stereotyping. Six studies with 4-year-old children (N = 214)…

Blood lead levels and risk factors in pregnant women from Durango, Mexico.

PubMed

La-Llave-León, Osmel; Estrada-Martínez, Sergio; Manuel Salas-Pacheco, José; Peña-Elósegui, Rocío; Duarte-Sustaita, Jaime; Candelas Rangel, Jorge-Luís; García Vargas, Gonzalo

2011-01-01

In this cross-sectional study the authors determined blood lead levels (BLLs) and some risk factors for lead exposure in pregnant women. Two hundred ninety-nine pregnant women receiving medical attention by the Secretary of Health, State of Durango, Mexico, participated in this study between 2007 and 2008. BLLs were evaluated with graphite furnace atomic absorption spectrometry. The authors used Student t test, 1-way analysis of variance (ANOVA), and linear regression as statistical treatments. BLLs ranged from 0.36 to 23.6 μg/dL (mean = 2.79 μg/dL, standard deviation = 2.14). Multivariate analysis showed that the main predictors of BLLs were working in a place where lead is used, using lead glazed pottery, and eating soil.

Leukemia-Associated Nup214 Fusion Proteins Disturb the XPO1-Mediated Nuclear-Cytoplasmic Transport Pathway and Thereby the NF-κB Signaling Pathway.

PubMed

Saito, Shoko; Cigdem, Sadik; Okuwaki, Mitsuru; Nagata, Kyosuke

2016-07-01

Nuclear-cytoplasmic transport through nuclear pore complexes is mediated by nuclear transport receptors. Previous reports have suggested that aberrant nuclear-cytoplasmic transport due to mutations or overexpression of nuclear pore complexes and nuclear transport receptors is closely linked to diseases. Nup214, a component of nuclear pore complexes, has been found as chimeric fusion proteins in leukemia. Among various Nup214 fusion proteins, SET-Nup214 and DEK-Nup214 have been shown to be engaged in tumorigenesis, but their oncogenic mechanisms remain unclear. In this study, we examined the functions of the Nup214 fusion proteins by focusing on their effects on nuclear-cytoplasmic transport. We found that SET-Nup214 and DEK-Nup214 interact with exportin-1 (XPO1)/CRM1 and nuclear RNA export factor 1 (NXF1)/TAP, which mediate leucine-rich nuclear export signal (NES)-dependent protein export and mRNA export, respectively. SET-Nup214 and DEK-Nup214 decreased the XPO1-mediated nuclear export of NES proteins such as cyclin B and proteins involved in the NF-κB signaling pathway by tethering XPO1 onto nuclear dots where Nup214 fusion proteins are localized. We also demonstrated that SET-Nup214 and DEK-Nup214 expression inhibited NF-κB-mediated transcription by abnormal tethering of the complex containing p65 and its inhibitor, IκB, in the nucleus. These results suggest that SET-Nup214 and DEK-Nup214 perturb the regulation of gene expression through alteration of the nuclear-cytoplasmic transport system. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Synergistic anticancer effects of cisplatin and histone deacetylase inhibitors (SAHA and TSA) on cholangiocarcinoma cell lines.

PubMed

Asgar, Md Ali; Senawong, Gulsiri; Sripa, Banchob; Senawong, Thanaset

2016-01-01

Clinical application of cisplatin against cholangiocarcinoma is often associated with resistance and toxicity posing urgent demand for combination therapy. In this study, we evaluated the combined anticancer effect of cisplatin and histone deacetylase inhibitors (HDACIs), suberoylanilide hydroxamic acid (SAHA) and trichostatin A (TSA), on the cholangiocarcinoma KKU-100 and KKU-M214 cell lines. Antiproliferative activity was evaluated using MTT assay. Apoptosis induction and cell cycle arrest were analyzed by flow cytometry. Cell cycle and apoptosis regulating proteins were evaluated by western blot analysis. MTT assay showed that cisplatin, SAHA and TSA dose-dependently reduced the viability of KKU-100 and KKU-M214 cells. The combination of cisplatin and HDACIs exerted significantly more cytotoxicity than the single drugs. Combination indices below 1.0 reflect synergism between cisplatin and HDACIs, leading to positive dose reductions of cisplatin and HDACIs. Cisplatin and HDACIs alone induced G0/G1 phase arrest in KKU-100 cells, but the drug combinations increased sub-G1 percent more than either drug. However, cisplatin and HDACIs alone or in combination increased only the sub-G1 percent in KKU-M214 cells. Annexin V-FITC staining revealed that cisplatin and HDACIs combinations induced more apoptotic cell death of both KKU-100 and KKU-M214 cells than the single drug. In KKU-100 cells, growth inhibition was accompanied by upregulation of p53 and p21 and downregulation of CDK4 and Bcl-2 due to exposure to cisplatin, SAHA and TSA alone or in combination. Moreover, combination of agents exerted higher impacts on protein expression. Single agents or combination did not affect p53 expression, however, combination of cisplatin and HDACIs increased the expression of p21 in KKU-M214 cells. Taken together, cisplatin and HDACIs combination may improve the therapeutic outcome in cholangiocarcinoma patients.

18 CFR 385.214 - Intervention (Rule 214).

Code of Federal Regulations, 2013 CFR

2013-04-01

... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Intervention (Rule 214..., Notices of Tariff or Rate Examination, Orders To Show Cause, Intervention, and Summary Disposition § 385.214 Intervention (Rule 214). (a) Filing. (1) The Secretary of Energy is a party to any proceeding upon...

18 CFR 385.214 - Intervention (Rule 214).

Code of Federal Regulations, 2011 CFR

2011-04-01

... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Intervention (Rule 214..., Notices of Tariff or Rate Examination, Orders To Show Cause, Intervention, and Summary Disposition § 385.214 Intervention (Rule 214). (a) Filing. (1) The Secretary of Energy is a party to any proceeding upon...

18 CFR 385.214 - Intervention (Rule 214).

Code of Federal Regulations, 2012 CFR

2012-04-01

... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Intervention (Rule 214..., Notices of Tariff or Rate Examination, Orders To Show Cause, Intervention, and Summary Disposition § 385.214 Intervention (Rule 214). (a) Filing. (1) The Secretary of Energy is a party to any proceeding upon...

18 CFR 385.214 - Intervention (Rule 214).

Code of Federal Regulations, 2014 CFR

2014-04-01

... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Intervention (Rule 214..., Notices of Tariff or Rate Examination, Orders To Show Cause, Intervention, and Summary Disposition § 385.214 Intervention (Rule 214). (a) Filing. (1) The Secretary of Energy is a party to any proceeding upon...

MicroRNA-214 Suppresses Gluconeogenesis by Targeting Activating Transcriptional Factor 4*

PubMed Central

Li, Kai; Zhang, Jin; Yu, Junjie; Liu, Bin; Guo, Yajie; Deng, Jiali; Chen, Shanghai; Wang, Chunxia; Guo, Feifan

2015-01-01

Although the gluconeogenesis pathway is already a target for the treatment of type 2 diabetes, the potential role of microRNAs (miRNAs) in gluconeogenesis remains unclear. Here, we investigated the physiological functions of miR-214 in gluconeogenesis. The expression of miR-214 was suppressed by glucagon via protein kinase A signaling in primary hepatocytes, and miR-214 was down-regulated in the livers of fasted, high fat diet-induced diabetic and leptin receptor-mutated (db/db) mice. The overexpression of miR-214 in primary hepatocytes suppressed glucose production, and silencing miR-214 reversed this effect. Gluconeogenesis was suppressed in the livers of mice injected with an adenovirus expressing miR-214 (Ad-miR-214). Additionally, Ad-miR-214 alleviated high fat diet-induced elevation of gluconeogenesis and hyperglycemia. Furthermore, we found that activating transcription factor 4 (ATF4), a reported target of miR-214, can reverse the suppressive effect of miR-214 on gluconeogenesis in primary hepatocytes, and this suppressive effect was blocked in liver-specific ATF4 knock-out mice. ATF4 regulated gluconeogenesis via affecting forkhead box protein O1 (FOXO1) transcriptional activity. Finally, liver-specific miR-214 transgenic mice exhibited suppressed gluconeogenesis and reduced expression of ATF4, phosphoenolpyruvate carboxykinase, and glucose-6-phosphatase in liver. Taken together, our results suggest that the miR-214-ATF4 axis is a novel pathway for the regulation of hepatic gluconeogenesis. PMID:25657009

MicroRNA-214 Promotes Apoptosis in Canine Hemangiosarcoma by Targeting the COP1-p53 Axis.

PubMed

Heishima, Kazuki; Mori, Takashi; Sakai, Hiroki; Sugito, Nobuhiko; Murakami, Mami; Yamada, Nami; Akao, Yukihiro; Maruo, Kohji

2015-01-01

MicroRNA-214 regulates both angiogenic function in endothelial cells and apoptosis in various cancers. However, the regulation and function of miR-214 is unclear in canine hemangiosarcoma, which is a spontaneous model of human angiosarcoma. The expression and functional roles of miR-214 in canine hemangiosarcoma were presently explored by performing miRNA TaqMan qRT-PCR and transfecting cells with synthetic microRNA. Here, we report that miR-214 was significantly down-regulated in the cell lines used and in clinical samples of canine hemangiosarcoma. Restoration of miR-214 expression reduced cell growth and induced apoptosis in canine hemangiosarcoma cell lines through transcriptional activation of p53-regulated genes although miR-214 had a slight effect of growth inhibition on normal endothelial cells. We identified COP1, which is a critical negative regulator of p53, as a novel direct target of miR-214. COP1 was overexpressed and the specific COP1 knockdown induced apoptosis through transcriptional activation of p53-regulated genes as well as did miR-214-transfection in HSA cell lines. Furthermore, p53 knockdown abolished the miR-214-COP1-mediated apoptosis; thus, miR-214 and COP1 regulated apoptosis through controlling p53 in HSA. In conclusion, miR-214 functioned as a tumor suppressor in canine hemangiosarcoma by inducing apoptosis through recovering the function of p53. miR-214 down-regulation and COP1 overexpression is likely to contribute to tumorigenesis of HSA. Therefore, targeting miR-214-COP1-p53 axis would possibly be a novel effective strategy for treatment of canine hemangiosarcoma and capable of being applied to the development of novel therapeutics for human angiosarcoma.

49 CFR 214.531 - Schedule of repairs; general.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Hi-Rail Vehicles § 214.531 Schedule of repairs; general. Except as provided in §§ 214.527(c)(5), 214.529, and 214.533, an on-track roadway maintenance machine or hi-rail vehicle that does not meet all... or hi-rail vehicle shall be placed out of on-track service. ...

MicroRNA-214 suppresses growth, migration and invasion through a novel target, high mobility group AT-hook 1, in human cervical and colorectal cancer cells.

PubMed

Chandrasekaran, Karthik Subramanian; Sathyanarayanan, Anusha; Karunagaran, Devarajan

2016-09-06

MicroRNA-214 (miR-214) has been shown to act as a tumour suppressor in human cervical and colorectal cancer cells. The aim of this study was to experimentally validate high mobility group AT-hook 1 as a novel target for miR-214-mediated suppression of growth and motility. HMGA1 and miR-214 expression levels were estimated in cervical and colorectal clinical specimens using qPCR. HMGA1 3' untranslated region luciferase assays were performed to validate HMGA1 as a target of miR-214. Effect of altering the expression of miR-214 or HMGA1 on proliferation, migration and invasion of human cervical and colorectal cancer cells was investigated. miR-214 expression was poor while that of HMGA1 was high in cervical and colorectal cancer tissues. miR-214-re-expression or HMGA1 downregulation inhibited proliferation, migration and invasion of cancer cells while miR-214 inhibition had opposite effects. miR-214 was demonstrated to bind to the wild-type 3' untranslated region of HMGA1 but not with its mutant. Low expression of miR-214 concurrent with elevated levels of HMGA1 may contribute to cervical and colorectal cancer progression. miR-214-mediated regulation of HMGA1 is a novel mechanism for its tumour-suppressive actions in human cervical and colorectal cancer cells and opens up avenues for novel therapeutic strategies for these two cancers.

MicroRNA-214 suppresses gluconeogenesis by targeting activating transcriptional factor 4.

PubMed

Li, Kai; Zhang, Jin; Yu, Junjie; Liu, Bin; Guo, Yajie; Deng, Jiali; Chen, Shanghai; Wang, Chunxia; Guo, Feifan

2015-03-27

Although the gluconeogenesis pathway is already a target for the treatment of type 2 diabetes, the potential role of microRNAs (miRNAs) in gluconeogenesis remains unclear. Here, we investigated the physiological functions of miR-214 in gluconeogenesis. The expression of miR-214 was suppressed by glucagon via protein kinase A signaling in primary hepatocytes, and miR-214 was down-regulated in the livers of fasted, high fat diet-induced diabetic and leptin receptor-mutated (db/db) mice. The overexpression of miR-214 in primary hepatocytes suppressed glucose production, and silencing miR-214 reversed this effect. Gluconeogenesis was suppressed in the livers of mice injected with an adenovirus expressing miR-214 (Ad-miR-214). Additionally, Ad-miR-214 alleviated high fat diet-induced elevation of gluconeogenesis and hyperglycemia. Furthermore, we found that activating transcription factor 4 (ATF4), a reported target of miR-214, can reverse the suppressive effect of miR-214 on gluconeogenesis in primary hepatocytes, and this suppressive effect was blocked in liver-specific ATF4 knock-out mice. ATF4 regulated gluconeogenesis via affecting forkhead box protein O1 (FOXO1) transcriptional activity. Finally, liver-specific miR-214 transgenic mice exhibited suppressed gluconeogenesis and reduced expression of ATF4, phosphoenolpyruvate carboxykinase, and glucose-6-phosphatase in liver. Taken together, our results suggest that the miR-214-ATF4 axis is a novel pathway for the regulation of hepatic gluconeogenesis. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Florida's timber industry-an assessment of timber product output and use, 2005

Treesearch

Tony G. Johnson; James W. Bentley; Michael Howell

2008-01-01

In 2005, volume of industrial roundwood output from Florida's forests totaled 445 million cubic feet, 13 percent less than in 2003. Mill byproducts generated from primary manufacturers declined to 146 million cubic feet. Almost all plant residues were used primarily for fuel and fiber products. Pulpwood was the leading roundwood product at 214 million cubic feet;...

Osteoclast-derived exosomal miR-214-3p inhibits osteoblastic bone formation

PubMed Central

Li, Defang; Liu, Jin; Guo, Baosheng; Liang, Chao; Dang, Lei; Lu, Cheng; He, Xiaojuan; Cheung, Hilda Yeuk-Siu; Xu, Liang; Lu, Changwei; He, Bing; Liu, Biao; Shaikh, Atik Badshah; Li, Fangfei; Wang, Luyao; Yang, Zhijun; Au, Doris Wai-Ting; Peng, Songlin; Zhang, Zongkang; Zhang, Bao-Ting; Pan, Xiaohua; Qian, Airong; Shang, Peng; Xiao, Lianbo; Jiang, Baohong; Wong, Chris Kong-Chu; Xu, Jiake; Bian, Zhaoxiang; Liang, Zicai; Guo, De-an; Zhu, Hailong; Tan, Weihong; Lu, Aiping; Zhang, Ge

2016-01-01

Emerging evidence indicates that osteoclasts direct osteoblastic bone formation. MicroRNAs (miRNAs) have a crucial role in regulating osteoclast and osteoblast function. However, whether miRNAs mediate osteoclast-directed osteoblastic bone formation is mostly unknown. Here, we show that increased osteoclastic miR-214-3p associates with both elevated serum exosomal miR-214-3p and reduced bone formation in elderly women with fractures and in ovariectomized (OVX) mice. Osteoclast-specific miR-214-3p knock-in mice have elevated serum exosomal miR-214-3p and reduced bone formation that is rescued by osteoclast-targeted antagomir-214-3p treatment. We further demonstrate that osteoclast-derived exosomal miR-214-3p is transferred to osteoblasts to inhibit osteoblast activity in vitro and reduce bone formation in vivo. Moreover, osteoclast-targeted miR-214-3p inhibition promotes bone formation in ageing OVX mice. Collectively, our results suggest that osteoclast-derived exosomal miR-214-3p transfers to osteoblasts to inhibit bone formation. Inhibition of miR-214-3p in osteoclasts may be a strategy for treating skeletal disorders involving a reduction in bone formation. PMID:26947250

MicroRNA-214-5p Inhibits the Invasion and Migration of Hepatocellular Carcinoma Cells by Targeting Wiskott-Aldrich Syndrome Like.

PubMed

Li, Hongdan; Wang, Haoqi; Ren, Zhen

2018-01-01

This study aims to explore the effects of microRNA-214-5p (miR-214-5p) on the invasion and migration of Hepatocellular Carcinoma cells (HCC). Hepatocellular Carcinoma tissues and adjacent normal tissues from 44 hepatocellular carcinoma patients were prepared for this study. The HepG2 and BEL-7402 cells were transfected with miR-214-5p mimic and inhibitor. qRT-PCR was performed to detect the expressions of miR-214-5p. Transwell assays were used to detect the invasion and migration assays in HepG2 and BEL-7402 cells. A dual-luciferase reporter assay was conducted to examine the effect of miR-214-5p on Wiskott-Aldrich Syndrome Like (WASL/ N-WASP). Western blot and qRT-PCR were used to measure the expressions of the E-cadherin, N-cadherin and Vimentin proteins. Transwell chamber assays were performed to detect cell invasion and migration. Compared with normal tissues, HCC tissues demonstrated significantly lower expression of miR-214-5p. Overexpression of miR-214-5p significantly inhibited the migration and invasion of HCC cells and inhibition of miR-214-5p promoted the migration and invasion. Additionally, miR-214-5p suppressed the epithelial-mesenchymal transition (EMT). Further study showed WASL was a putative target gene of miR-214-5p. Up-regulating the expression of WASL could reverse the inhibition effect of miR-214-5p on invasion and migration. Our data suggested that miR-214-5p inhibited the invasion and migration of HepG2 and BEL-7402 by targeting WASL in Hepatocellular carcinoma. © 2018 The Author(s). Published by S. Karger AG, Basel.

miR-214 promotes the proliferation and invasion of osteosarcoma cells through direct suppression of LZTS1

DOE Office of Scientific and Technical Information (OSTI.GOV)

Xu, Zhengyu; Wang, Tao, E-mail: wangtaohappy2010@sohu.com

2014-06-27

Highlights: • miR-214 is upregulated in human OS tissues and inversely correlated with LZTS1 expression. • miR-214 directly targets LZTS1 by binding to its 3′-UTR. • miR-214 promotes OS cell proliferation, invasion and tumor growth. • Overexpression of LZTS1 reverses miR-214-induced proliferation and invasion of OS cells. - Abstract: Previous studies have shown that miR-214 functions either as an oncogene or a tumor suppressor in various human cancer types. The role of this microRNA in osteosarcoma (OS) is presently unclear. Here, we demonstrated that miR-214 is frequently upregulated in OS specimens, compared with noncancerous bone tissues. Bioinformatics analysis further revealedmore » leucine zipper, putative tumor suppressor 1 (LZTS1) as a potential target of miR-214. Expression patterns of miR-214 were inversely correlated with those of LZTS1 mRNA and protein in OS tissues. Data from reporter assays showed that miR-214 directly binds to the 3′-untranslated region (3′-UTR) of LZTS1 mRNA and suppresses expression at both transcriptional and translational levels. In functional assays, miR-214 promoted OS cell proliferation, invasion and tumor growth in nude mice, which could be reversed by overexpression of LZTS1. Taken together, our data provide compelling evidence that miR-214 functions as an onco-miRNA in OS, and its oncogenic effects are mediated chiefly through downregulation of LZTS1.« less

78 FR 60182 - Airworthiness Directives; Bell Helicopter Textron, Inc., Helicopters

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-01

... Helicopter Textron, Inc. (Bell), Model 214B, 214B-1, and 214ST helicopters. This AD requires creating a... and 214B-1 helicopters with a certain spindle installed. The NPRM proposed to require creating a...

49 CFR 214.305 - Compliance dates.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Compliance dates. 214.305 Section 214.305 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.305 Compliance dates...

Mesotrypsin Signature Mutation in a Chymotrypsin C (CTRC) Variant Associated with Chronic Pancreatitis.

PubMed

Szabó, András; Ludwig, Maren; Hegyi, Eszter; Szépeová, Renata; Witt, Heiko; Sahin-Tóth, Miklós

2015-07-10

Human chymotrypsin C (CTRC) protects against pancreatitis by degrading trypsinogen and thereby curtailing harmful intra-pancreatic trypsinogen activation. Loss-of-function mutations in CTRC increase the risk for chronic pancreatitis. Here we describe functional analysis of eight previously uncharacterized natural CTRC variants tested for potential defects in secretion, proteolytic stability, and catalytic activity. We found that all variants were secreted from transfected cells normally, and none suffered proteolytic degradation by trypsin. Five variants had normal enzymatic activity, whereas variant p.R29Q was catalytically inactive due to loss of activation by trypsin and variant p.S239C exhibited impaired activity possibly caused by disulfide mispairing. Surprisingly, variant p.G214R had increased activity on a small chromogenic peptide substrate but was markedly defective in cleaving bovine β-casein or the natural CTRC substrates human cationic trypsinogen and procarboxypeptidase A1. Mutation p.G214R is analogous to the evolutionary mutation in human mesotrypsin, which rendered this trypsin isoform resistant to proteinaceous inhibitors and conferred its ability to cleave these inhibitors. Similarly to the mesotrypsin phenotype, CTRC variant p.G214R was inhibited poorly by eglin C, ecotin, or a CTRC-specific variant of SGPI-2, and it readily cleaved the reactive-site peptide bonds in eglin C and ecotin. We conclude that CTRC variants p.R29Q, p.G214R, and p.S239C are risk factors for chronic pancreatitis. Furthermore, the mesotrypsin-like CTRC variant highlights how the same natural mutation in homologous pancreatic serine proteases can evolve a new physiological role or lead to pathology, determined by the biological context of protease function. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

Synthesis, X-ray crystal structures, and gas sorption properties of pillared square grid nets based on paddle-wheel motifs: implications for hydrogen storage in porous materials.

PubMed

Chun, Hyungphil; Dybtsev, Danil N; Kim, Hyunuk; Kim, Kimoon

2005-06-06

A systematic modulation of organic ligands connecting dinuclear paddle-wheel motifs leads to a series of isomorphous metal-organic porous materials that have a three-dimensional connectivity and interconnected pores. Aromatic dicarboxylates such as 1,4-benzenedicarboxylate (1,4-bdc), tetramethylterephthalate (tmbdc), 1,4-naphthalenedicarboxylate (1,4-ndc), tetrafluoroterephthalate (tfbdc), or 2,6-naphthalenedicarboxylate (2,6-ndc) are linear linkers that form two-dimensional layers, and diamine ligands, 4-diazabicyclo[2.2.2]octane (dabco) or 4,4'-dipyridyl (bpy), coordinate at both sides of Zn(2) paddle-wheel units to bridge the layers vertically. The resulting open frameworks [Zn(2)(1,4-bdc)(2)(dabco)] (1), [Zn(2)(1,4-bdc)(tmbdc)(dabco)] (2), [Zn(2)(tmbdc)(2)(dabco)] (3), [Zn(2)(1,4-ndc)(2)(dabco)] (4), [Zn(2)(tfbdc)(2)(dabco)] (5), and [Zn(2)(tmbdc)(2)(bpy)] (8) possess varying size of pores and free apertures originating from the side groups of the 1,4-bdc derivatives. [Zn(2)(1,4-bdc)(2)(bpy)] (6) and [Zn(2)(2,6-ndc)(2)(bpy)] (7) have two- and threefold interpenetrating structures, respectively. The non-interpenetrating frameworks (1-5 and 8) possess surface areas in the range of 1450-2090 m(2)g(-1) and hydrogen sorption capacities of 1.7-2.1 wt % at 78 K and 1 atm. A detailed analysis of the sorption data in conjunction with structural similarities and differences concludes that porous materials with straight channels and large openings do not perform better than those with wavy channels and small openings in terms of hydrogen storage through physisorption.

48 CFR 5433.214. - Alternative Dispute Resolution (ADR).

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 7 2011-10-01 2011-10-01 false Alternative Dispute Resolution (ADR). 5433.214. Section 5433.214. Federal Acquisition Regulations System DEFENSE LOGISTICS AGENCY, DEPARTMENT OF DEFENSE PROTESTS, DISPUTES AND APPEALS 5433.214. Alternative Dispute Resolution (ADR). The...

48 CFR 5433.214. - Alternative Dispute Resolution (ADR).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Alternative Dispute Resolution (ADR). 5433.214. Section 5433.214. Federal Acquisition Regulations System DEFENSE LOGISTICS AGENCY, DEPARTMENT OF DEFENSE PROTESTS, DISPUTES AND APPEALS 5433.214. Alternative Dispute Resolution (ADR). The...

49 CFR 214.4 - Preemptive effect.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Preemptive effect. 214.4 Section 214.4 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY General § 214.4 Preemptive effect. Under 49 U.S.C...

28 CFR 2.14 - Subsequent proceedings.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 28 Judicial Administration 1 2011-07-01 2011-07-01 false Subsequent proceedings. 2.14 Section 2.14 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF PRISONERS, YOUTH OFFENDERS, AND JUVENILE DELINQUENTS United States Code Prisoners and Parolees § 2.14 Subsequent...

28 CFR 2.14 - Subsequent proceedings.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Subsequent proceedings. 2.14 Section 2.14 Judicial Administration DEPARTMENT OF JUSTICE PAROLE, RELEASE, SUPERVISION AND RECOMMITMENT OF PRISONERS, YOUTH OFFENDERS, AND JUVENILE DELINQUENTS United States Code Prisoners and Parolees § 2.14 Subsequent...

48 CFR 5433.214. - Alternative Dispute Resolution (ADR).

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Alternative Dispute Resolution (ADR). 5433.214. Section 5433.214. Federal Acquisition Regulations System DEFENSE LOGISTICS AGENCY, DEPARTMENT OF DEFENSE PROTESTS, DISPUTES AND APPEALS 5433.214. Alternative Dispute Resolution (ADR). The...

48 CFR 5433.214. - Alternative Dispute Resolution (ADR).

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 7 2014-10-01 2014-10-01 false Alternative Dispute Resolution (ADR). 5433.214. Section 5433.214. Federal Acquisition Regulations System DEFENSE LOGISTICS AGENCY, DEPARTMENT OF DEFENSE PROTESTS, DISPUTES AND APPEALS 5433.214. Alternative Dispute Resolution (ADR). The...

48 CFR 5433.214. - Alternative Dispute Resolution (ADR).

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Alternative Dispute Resolution (ADR). 5433.214. Section 5433.214. Federal Acquisition Regulations System DEFENSE LOGISTICS AGENCY, DEPARTMENT OF DEFENSE PROTESTS, DISPUTES AND APPEALS 5433.214. Alternative Dispute Resolution (ADR). The...

24 CFR 214.303 - Performance criteria.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Performance criteria. 214.303 Section 214.303 Housing and Urban Development Regulations Relating to Housing and Urban Development... HOUSING COUNSELING PROGRAM Program Administration § 214.303 Performance criteria. To maintain HUD-approved...

24 CFR 214.303 - Performance criteria.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Performance criteria. 214.303 Section 214.303 Housing and Urban Development Regulations Relating to Housing and Urban Development... HOUSING COUNSELING PROGRAM Program Administration § 214.303 Performance criteria. To maintain HUD-approved...

29 CFR 1910.214 - Cooperage machinery. [Reserved

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 5 2011-07-01 2011-07-01 false Cooperage machinery. [Reserved] 1910.214 Section 1910.214 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR OCCUPATIONAL SAFETY AND HEALTH STANDARDS Machinery and Machine Guarding § 1910.214...

48 CFR 12.214 - Cost Accounting Standards.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Cost Accounting Standards. 12.214 Section 12.214 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... Items 12.214 Cost Accounting Standards. Cost Accounting Standards (CAS) do not apply to contracts and...

MiR-214 regulates oral cancer KB cell apoptosis through targeting RASSF5.

PubMed

Li, T K; Yin, K; Chen, Z; Bao, Y; Zhang, S X

2017-03-08

Ras association domain family member 5 (RASSF5), a member of the Ras association domain family, induces cell apoptosis by phosphorylating FOXO3a, which triggers target gene BIM (pro-apoptotic factor) activation. MiR-214 is overexpressed in oral cancer tissue, indicating its possible involvement in oral cancer pathogenesis. Bioinformatics analysis has revealed a complimentary sequence between miR-214 and the 3'-UTR of RASSF5 mRNA. However, whether miR-124 regulates RASSF5 in oral cancer remains poorly understood. We aimed to investigate the role of miR-214 in RASSF5 expression regulation in oral cancer. Tumor and paracarcinoma tissues were obtained from 48 oral cancer patients to examine miR-214 and RASSF5 expression. The relationship between miR-214 and RASSF5 was investigated by dual luciferase reporter gene assay. Oral cancer KB cells were cultured in vitro and divided into inhibitor NC, miR-214 inhibitor, Scramble-pMD18, RASSF5-pMD18, and miR-214 inhibitor + RASSF5-pMD18 groups. Caspase 3 activity, cell apoptosis, and total protein expression were measured by spectrophotometry, flow cytometry, and western blot, respectively. MiR-214 expression was significantly increased, while that of RASSF5 decreased in oral cancer tumor tissues compared to paracarcinoma tissues. Luciferase assay showed that miR-214 suppressed RASSF5 expression by targeting its 3'-UTR. Down-regulation of miR-214 and/or enhancement of RASSF5 expression markedly increased FOXO3a phosphorylation, BIM expression, caspase 3 activity, and apoptosis. In conclusion, miR-214 expression was elevated and RASSF5 was down-regulated in oral cancer. Moreover, miR-214 regulated KB cell apoptosis through targeted inhibition of RASSF5 expression, FOXO3a phosphorylation, and BIM expression, suggesting its possible application as a novel therapeutic oral cancer target.

45 CFR 144.214 - Notifications of noncompliance with reporting requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... requirements. 144.214 Section 144.214 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH CARE ACCESS REQUIREMENTS RELATING TO HEALTH INSURANCE COVERAGE Qualified State Long-Term Care Insurance Partnerships: Reporting Requirements for Insurers § 144.214 Notifications of...

22 CFR 214.12 - Considerations in membership selection.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Considerations in membership selection. 214.12 Section 214.12 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Establishment of Advisory Committees § 214.12 Considerations in membership selection. Membership is to be fairly...

48 CFR 214.202-5 - Descriptive literature.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Descriptive literature. 214.202-5 Section 214.202-5 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS... 214.202-5 Descriptive literature. (c) Requirements of invitation for bids. When brand name or equal...

48 CFR 214.202-5 - Descriptive literature.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Descriptive literature. 214.202-5 Section 214.202-5 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS... 214.202-5 Descriptive literature. (c) Requirements of invitation for bids. When brand name or equal...

48 CFR 214.202-5 - Descriptive literature.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Descriptive literature. 214.202-5 Section 214.202-5 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS... 214.202-5 Descriptive literature. (c) Requirements of invitation for bids. When brand name or equal...

48 CFR 214.202-5 - Descriptive literature.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Descriptive literature. 214.202-5 Section 214.202-5 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS... 214.202-5 Descriptive literature. (c) Requirements of invitation for bids. When brand name or equal...

48 CFR 214.202-5 - Descriptive literature.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Descriptive literature. 214.202-5 Section 214.202-5 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS... 214.202-5 Descriptive literature. (c) Requirements of invitation for bids. When brand name or equal...

45 CFR 144.214 - Notifications of noncompliance with reporting requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... requirements. 144.214 Section 144.214 Public Welfare Department of Health and Human Services REQUIREMENTS RELATING TO HEALTH CARE ACCESS REQUIREMENTS RELATING TO HEALTH INSURANCE COVERAGE Qualified State Long-Term Care Insurance Partnerships: Reporting Requirements for Insurers § 144.214 Notifications of...

45 CFR 144.214 - Notifications of noncompliance with reporting requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... requirements. 144.214 Section 144.214 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH CARE ACCESS REQUIREMENTS RELATING TO HEALTH INSURANCE COVERAGE Qualified State Long-Term Care Insurance Partnerships: Reporting Requirements for Insurers § 144.214 Notifications of...

45 CFR 144.214 - Notifications of noncompliance with reporting requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... requirements. 144.214 Section 144.214 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH CARE ACCESS REQUIREMENTS RELATING TO HEALTH INSURANCE COVERAGE Qualified State Long-Term Care Insurance Partnerships: Reporting Requirements for Insurers § 144.214 Notifications of...

22 CFR 1203.735-214 - Transmitting communications and gifts.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Transmitting communications and gifts. 1203.735-214 Section 1203.735-214 Foreign Relations UNITED STATES INTERNATIONAL DEVELOPMENT COOPERATION AGENCY....735-214 Transmitting communications and gifts. (a) Correspondence. In corresponding with anyone other...

24 CFR 214.311 - Funding.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Funding. 214.311 Section 214.311... PROGRAM Program Administration § 214.311 Funding. (a) HUD funding. HUD approval or program participation does not guarantee funding from HUD. Funding for the Housing Counseling program depends on...

48 CFR 3033.214 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2010 CFR

2010-10-01

... resolution (ADR). 3033.214 Section 3033.214 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND..., DISPUTES, AND APPEALS Disputes and Appeals 3033.214 Alternative dispute resolution (ADR). (c) The... informal resolution of disputes, and for other purposes. CBCA guidance on ADR may be obtained at http://www...

48 CFR 3033.214 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2011 CFR

2011-10-01

... resolution (ADR). 3033.214 Section 3033.214 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND..., DISPUTES, AND APPEALS Disputes and Appeals 3033.214 Alternative dispute resolution (ADR). (c) The... informal resolution of disputes, and for other purposes. CBCA guidance on ADR may be obtained at http://www...

49 CFR 214.325 - Train coordination.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Train coordination. 214.325 Section 214.325..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.325 Train coordination. Working limits established by a roadway worker through the use of train coordination shall comply with the...

49 CFR 214.325 - Train coordination.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Train coordination. 214.325 Section 214.325..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.325 Train coordination. Working limits established by a roadway worker through the use of train coordination shall comply with the...

47 CFR 214.0 - Authority.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Authority. 214.0 Section 214.0 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.0 Authority. The provisions of this part...

47 CFR 214.2 - Scope.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Scope. 214.2 Section 214.2 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.2 Scope. This part covers procedures for...

22 CFR 214.32 - Calling of advisory committee meetings.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Calling of advisory committee meetings. 214.32 Section 214.32 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Operation of Advisory Committees § 214.32 Calling of advisory committee meetings. (a) No advisory committee...

22 CFR 214.41 - Support services.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Support services. 214.41 Section 214.41 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Administration of Advisory Committees § 214.41 Support services. (a) A.I.D. provides support services for advisory committees which are...

46 CFR 10.214 - Security Check.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 1 2011-10-01 2011-10-01 false Security Check. 10.214 Section 10.214 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MERCHANT MARINER CREDENTIAL General Requirements for All Merchant Mariner Credentials § 10.214 Security Check. Until April 15, 2009...

46 CFR 10.214 - Security Check.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 1 2010-10-01 2010-10-01 false Security Check. 10.214 Section 10.214 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY MERCHANT MARINE OFFICERS AND SEAMEN MERCHANT MARINER CREDENTIAL General Requirements for All Merchant Mariner Credentials § 10.214 Security Check. Until April 15, 2009...

47 CFR 214.3 - Assumptions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Assumptions. 214.3 Section 214.3 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.3 Assumptions. When the provisions of this...

49 CFR 214.323 - Foul time.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Foul time. 214.323 Section 214.323 Transportation... TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.323 Foul time. Working limits established on controlled track through the use of foul time procedures shall comply with the following...

49 CFR 214.323 - Foul time.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Foul time. 214.323 Section 214.323 Transportation... TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.323 Foul time. Working limits established on controlled track through the use of foul time procedures shall comply with the following...

49 CFR 214.323 - Foul time.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Foul time. 214.323 Section 214.323 Transportation... TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.323 Foul time. Working limits established on controlled track through the use of foul time procedures shall comply with the following...

47 CFR 214.2 - Scope.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Scope. 214.2 Section 214.2 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.2 Scope. This part covers procedures for...

47 CFR 214.0 - Authority.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Authority. 214.0 Section 214.0 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.0 Authority. The provisions of this part...

47 CFR 214.0 - Authority.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Authority. 214.0 Section 214.0 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.0 Authority. The provisions of this part...

47 CFR 214.0 - Authority.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Authority. 214.0 Section 214.0 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.0 Authority. The provisions of this part...

47 CFR 214.3 - Assumptions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Assumptions. 214.3 Section 214.3 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.3 Assumptions. When the provisions of this...

47 CFR 214.2 - Scope.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Scope. 214.2 Section 214.2 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.2 Scope. This part covers procedures for...

47 CFR 214.2 - Scope.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Scope. 214.2 Section 214.2 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.2 Scope. This part covers procedures for...

47 CFR 214.3 - Assumptions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Assumptions. 214.3 Section 214.3 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.3 Assumptions. When the provisions of this...

47 CFR 214.0 - Authority.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Authority. 214.0 Section 214.0 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.0 Authority. The provisions of this part...

47 CFR 214.3 - Assumptions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Assumptions. 214.3 Section 214.3 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.3 Assumptions. When the provisions of this...

47 CFR 214.3 - Assumptions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Assumptions. 214.3 Section 214.3 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.3 Assumptions. When the provisions of this...

47 CFR 214.2 - Scope.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Scope. 214.2 Section 214.2 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.2 Scope. This part covers procedures for...

22 CFR 214.22 - Responsibilities within A.I.D.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Responsibilities within A.I.D. 214.22 Section 214.22 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Termination and Renewal of Advisory Committees § 214.22 Responsibilities within A.I.D. Responsibilities within...

22 CFR 214.22 - Responsibilities within A.I.D.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Responsibilities within A.I.D. 214.22 Section 214.22 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Termination and Renewal of Advisory Committees § 214.22 Responsibilities within A.I.D. Responsibilities within...

22 CFR 214.22 - Responsibilities within A.I.D.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Responsibilities within A.I.D. 214.22 Section 214.22 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Termination and Renewal of Advisory Committees § 214.22 Responsibilities within A.I.D. Responsibilities within...

22 CFR 214.22 - Responsibilities within A.I.D.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Responsibilities within A.I.D. 214.22 Section 214.22 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Termination and Renewal of Advisory Committees § 214.22 Responsibilities within A.I.D. Responsibilities within...

45 CFR 144.214 - Notifications of noncompliance with reporting requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... requirements. 144.214 Section 144.214 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH CARE ACCESS REQUIREMENTS RELATING TO HEALTH INSURANCE COVERAGE Source: § 144.214 Notifications of noncompliance with reporting requirements. If an insurer of a qualified long-term care...

20 CFR 802.214 - Intervention.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false Intervention. 802.214 Section 802.214 Employees' Benefits BENEFITS REVIEW BOARD, DEPARTMENT OF LABOR RULES OF PRACTICE AND PROCEDURE Prereview Procedures Initial Processing § 802.214 Intervention. (a) If a person or legal entity shows in a written...

20 CFR 802.214 - Intervention.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 4 2014-04-01 2014-04-01 false Intervention. 802.214 Section 802.214 Employees' Benefits BENEFITS REVIEW BOARD, DEPARTMENT OF LABOR RULES OF PRACTICE AND PROCEDURE Prereview Procedures Initial Processing § 802.214 Intervention. (a) If a person or legal entity shows in a written...

20 CFR 802.214 - Intervention.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 20 Employees' Benefits 4 2013-04-01 2013-04-01 false Intervention. 802.214 Section 802.214 Employees' Benefits BENEFITS REVIEW BOARD, DEPARTMENT OF LABOR RULES OF PRACTICE AND PROCEDURE Prereview Procedures Initial Processing § 802.214 Intervention. (a) If a person or legal entity shows in a written...

20 CFR 802.214 - Intervention.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 4 2012-04-01 2012-04-01 false Intervention. 802.214 Section 802.214 Employees' Benefits BENEFITS REVIEW BOARD, DEPARTMENT OF LABOR RULES OF PRACTICE AND PROCEDURE Prereview Procedures Initial Processing § 802.214 Intervention. (a) If a person or legal entity shows in a written...

20 CFR 802.214 - Intervention.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false Intervention. 802.214 Section 802.214 Employees' Benefits BENEFITS REVIEW BOARD, DEPARTMENT OF LABOR RULES OF PRACTICE AND PROCEDURE Prereview Procedures Initial Processing § 802.214 Intervention. (a) If a person or legal entity shows in a written...

24 CFR 884.214 - Marketing.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 4 2013-04-01 2013-04-01 false Marketing. 884.214 Section 884.214... HOUSING PROJECTS Project Development and Operation § 884.214 Marketing. (a) Compliance with equal opportunity requirements. Marketing of units and selection of Families by the Owner shall be in accordance...

24 CFR 884.214 - Marketing.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 4 2010-04-01 2010-04-01 false Marketing. 884.214 Section 884.214... HOUSING PROJECTS Project Development and Operation § 884.214 Marketing. (a) Compliance with equal opportunity requirements. Marketing of units and selection of Families by the Owner shall be in accordance...

24 CFR 884.214 - Marketing.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 4 2011-04-01 2011-04-01 false Marketing. 884.214 Section 884.214... HOUSING PROJECTS Project Development and Operation § 884.214 Marketing. (a) Compliance with equal opportunity requirements. Marketing of units and selection of Families by the Owner shall be in accordance...

24 CFR 884.214 - Marketing.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 4 2014-04-01 2014-04-01 false Marketing. 884.214 Section 884.214... HOUSING PROJECTS Project Development and Operation § 884.214 Marketing. (a) Compliance with equal opportunity requirements. Marketing of units and selection of Families by the Owner shall be in accordance...

24 CFR 884.214 - Marketing.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 4 2012-04-01 2012-04-01 false Marketing. 884.214 Section 884.214... HOUSING PROJECTS Project Development and Operation § 884.214 Marketing. (a) Compliance with equal opportunity requirements. Marketing of units and selection of Families by the Owner shall be in accordance...

40 CFR 21.4 - Review of application.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 1 2011-07-01 2011-07-01 false Review of application. 21.4 Section 21.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL SMALL BUSINESS § 21.4 Review of..., efficiency, or technological standpoint. (c) An application which proposes additions, alterations, or methods...

40 CFR 21.4 - Review of application.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 1 2013-07-01 2013-07-01 false Review of application. 21.4 Section 21.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL SMALL BUSINESS § 21.4 Review of..., efficiency, or technological standpoint. (c) An application which proposes additions, alterations, or methods...

40 CFR 21.4 - Review of application.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 1 2014-07-01 2014-07-01 false Review of application. 21.4 Section 21.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL SMALL BUSINESS § 21.4 Review of..., efficiency, or technological standpoint. (c) An application which proposes additions, alterations, or methods...

40 CFR 21.4 - Review of application.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 1 2012-07-01 2012-07-01 false Review of application. 21.4 Section 21.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL SMALL BUSINESS § 21.4 Review of..., efficiency, or technological standpoint. (c) An application which proposes additions, alterations, or methods...

40 CFR 21.4 - Review of application.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Review of application. 21.4 Section 21.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GENERAL SMALL BUSINESS § 21.4 Review of..., efficiency, or technological standpoint. (c) An application which proposes additions, alterations, or methods...

33 CFR 214.11 - Costs.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Costs. 214.11 Section 214.11... SUPPLIES OF DRINKING WATER § 214.11 Costs. Costs incurred by the Corps of Engineers in furnishing emergency... by the community generally will not be required. Costs of necessary measures for the decontamination...

47 CFR 214.1 - Purpose.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Purpose. 214.1 Section 214.1 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.1 Purpose. The purpose of this part is to...

18 CFR 281.214 - Notice, complaint and remedy.

Code of Federal Regulations, 2010 CFR

2010-04-01

... remedy. 281.214 Section 281.214 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY....214 Notice, complaint and remedy. (a) Complaint. Any interested person may file a complaint concerning an alleged violation of this subpart under § 385.206 of this chapter. (b) Remedy. If the Commission...

13 CFR 120.214 - What conditions apply for variable interest rates?

Code of Federal Regulations, 2010 CFR

2010-01-01

... interest rates? 120.214 Section 120.214 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Policies Specific to 7(a) Loans Maturities; Interest Rates; Loan and Guarantee Amounts § 120.214 What conditions apply for variable interest rates? A Lender may use a variable rate of interest...

42 CFR 438.214 - Provider selection.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 4 2010-10-01 2010-10-01 false Provider selection. 438.214 Section 438.214 Public... Operation Standards § 438.214 Provider selection. (a) General rules. The State must ensure, through its contracts, that each MCO, PIHP, or PAHP implements written policies and procedures for selection and...

49 CFR 214.117 - Eye and face protection.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Eye and face protection. 214.117 Section 214.117..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Bridge Worker Safety Standards § 214.117 Eye and face protection. (a) Railroad bridge workers shall be provided and shall wear eye and face protection equipment...

49 CFR 214.117 - Eye and face protection.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Eye and face protection. 214.117 Section 214.117..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Bridge Worker Safety Standards § 214.117 Eye and face protection. (a) Railroad bridge workers shall be provided and shall wear eye and face protection equipment...

22 CFR 214.35 - Minutes of meetings.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Minutes of meetings. 214.35 Section 214.35 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Operation of Advisory Committees § 214.35 Minutes of meetings. (a) Minutes are to be kept of each meeting of each advisory...

34 CFR 685.214 - Closed school discharge.

Code of Federal Regulations, 2014 CFR

2014-07-01

... closure date is the date that the school ceases to provide educational instruction in all programs, as... 34 Education 4 2014-07-01 2014-07-01 false Closed school discharge. 685.214 Section 685.214... Provisions § 685.214 Closed school discharge. (a) General. (1) The Secretary discharges the borrower's (and...

8 CFR 214.14 - Alien victims of certain qualifying criminal activity.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 8 Aliens and Nationality 1 2013-01-01 2013-01-01 false Alien victims of certain qualifying criminal activity. 214.14 Section 214.14 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS NONIMMIGRANT CLASSES § 214.14 Alien victims of certain qualifying criminal activity. (a...

8 CFR 214.14 - Alien victims of certain qualifying criminal activity.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 8 Aliens and Nationality 1 2014-01-01 2014-01-01 false Alien victims of certain qualifying criminal activity. 214.14 Section 214.14 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS NONIMMIGRANT CLASSES § 214.14 Alien victims of certain qualifying criminal activity. (a...

8 CFR 214.14 - Alien victims of certain qualifying criminal activity.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 8 Aliens and Nationality 1 2011-01-01 2011-01-01 false Alien victims of certain qualifying criminal activity. 214.14 Section 214.14 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS NONIMMIGRANT CLASSES § 214.14 Alien victims of certain qualifying criminal activity. (a...

8 CFR 214.14 - Alien victims of certain qualifying criminal activity.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 8 Aliens and Nationality 1 2012-01-01 2012-01-01 false Alien victims of certain qualifying criminal activity. 214.14 Section 214.14 Aliens and Nationality DEPARTMENT OF HOMELAND SECURITY IMMIGRATION REGULATIONS NONIMMIGRANT CLASSES § 214.14 Alien victims of certain qualifying criminal activity. (a...

22 CFR 214.2 - Definition of advisory committee.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Definition of advisory committee. 214.2 Section 214.2 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT General § 214.2 Definition of advisory committee. (a) The term advisory committee is defined in section 3(2) of...

22 CFR 214.3 - A.I.D. Advisory Committee Management Officer.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false A.I.D. Advisory Committee Management Officer. 214.3 Section 214.3 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT General § 214.3 A.I.D. Advisory Committee Management Officer. The Advisory Committee Management...

22 CFR 214.52 - Administrative review of other alleged non-compliance.

Code of Federal Regulations, 2010 CFR

2010-04-01

...-compliance. 214.52 Section 214.52 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Administrative Remedies § 214.52 Administrative review of other alleged non-compliance. With... International Development, 21st and Virginia Avenue, NW., Washington, DC 20523. (c) The complaint must be filed...

24 CFR 5.214 - Definitions.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Definitions. 5.214 Section 5.214... Identification Numbers; Procedures for Obtaining Income Information § 5.214 Definitions. In addition to the definitions in § 5.100, the following definitions apply to this subpart B: Assistance applicant. Except as...

33 CFR 214.5 - Policy.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Policy. 214.5 Section 214.5 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE EMERGENCY SUPPLIES OF DRINKING WATER § 214.5 Policy. Emergency work under this authority will be applied to...

49 CFR 195.214 - Welding procedures.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 3 2010-10-01 2010-10-01 false Welding procedures. 195.214 Section 195.214... PIPELINE Construction § 195.214 Welding procedures. (a) Welding must be performed by a qualified welder in accordance with welding procedures qualified under Section 5 of API 1104 or Section IX of the ASME Boiler and...

42 CFR 1002.214 - Basis for reinstatement after State agency-initiated exclusion.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 5 2010-10-01 2010-10-01 false Basis for reinstatement after State agency-initiated exclusion. 1002.214 Section 1002.214 Public Health OFFICE OF INSPECTOR GENERAL-HEALTH CARE... MEDICAID Permissive Exclusions § 1002.214 Basis for reinstatement after State agency-initiated exclusion...

23 CFR 646.214 - Design.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 23 Highways 1 2011-04-01 2011-04-01 false Design. 646.214 Section 646.214 Highways FEDERAL HIGHWAY... Projects § 646.214 Design. (a) General. (1) Facilities that are the responsibility of the railroad for maintenance and operation shall conform to the specifications and design standards used by the railroad in its...

34 CFR 300.214-300.219 - [Reserved

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 2 2010-07-01 2010-07-01 false [Reserved] 300.214-300.219 Section 300.214-300.219 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... CHILDREN WITH DISABILITIES Local Educational Agency Eligibility §§ 300.214-300.219 [Reserved] ...

34 CFR 300.214-300.219 - [Reserved

Code of Federal Regulations, 2014 CFR

2014-07-01

... 34 Education 2 2014-07-01 2013-07-01 true [Reserved] 300.214-300.219 Section 300.214-300.219 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... CHILDREN WITH DISABILITIES Local Educational Agency Eligibility §§ 300.214-300.219 [Reserved] ...

34 CFR 300.214-300.219 - [Reserved

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 2 2011-07-01 2010-07-01 true [Reserved] 300.214-300.219 Section 300.214-300.219 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... CHILDREN WITH DISABILITIES Local Educational Agency Eligibility §§ 300.214-300.219 [Reserved] ...

34 CFR 300.214-300.219 - [Reserved

Code of Federal Regulations, 2012 CFR

2012-07-01

... 34 Education 2 2012-07-01 2012-07-01 false [Reserved] 300.214-300.219 Section 300.214-300.219 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... CHILDREN WITH DISABILITIES Local Educational Agency Eligibility §§ 300.214-300.219 [Reserved] ...

34 CFR 300.214-300.219 - [Reserved

Code of Federal Regulations, 2013 CFR

2013-07-01

... 34 Education 2 2013-07-01 2013-07-01 false [Reserved] 300.214-300.219 Section 300.214-300.219 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION... CHILDREN WITH DISABILITIES Local Educational Agency Eligibility §§ 300.214-300.219 [Reserved] ...

42 CFR 59.214 - Additional conditions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 42 Public Health 1 2014-10-01 2014-10-01 false Additional conditions. 59.214 Section 59.214 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR FAMILY PLANNING SERVICES Grants for Family Planning Service Training § 59.214 Additional conditions. The Secretary may with...

42 CFR 59.214 - Additional conditions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 42 Public Health 1 2013-10-01 2013-10-01 false Additional conditions. 59.214 Section 59.214 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR FAMILY PLANNING SERVICES Grants for Family Planning Service Training § 59.214 Additional conditions. The Secretary may with...

42 CFR 59.214 - Additional conditions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 1 2010-10-01 2010-10-01 false Additional conditions. 59.214 Section 59.214 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR FAMILY PLANNING SERVICES Grants for Family Planning Service Training § 59.214 Additional conditions. The Secretary may with...

42 CFR 59.214 - Additional conditions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 42 Public Health 1 2012-10-01 2012-10-01 false Additional conditions. 59.214 Section 59.214 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR FAMILY PLANNING SERVICES Grants for Family Planning Service Training § 59.214 Additional conditions. The Secretary may with...

42 CFR 59.214 - Additional conditions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 42 Public Health 1 2011-10-01 2011-10-01 false Additional conditions. 59.214 Section 59.214 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES GRANTS GRANTS FOR FAMILY PLANNING SERVICES Grants for Family Planning Service Training § 59.214 Additional conditions. The Secretary may with...

49 CFR 214.513 - Retrofitting of existing on-track roadway maintenance machines; general.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Retrofitting of existing on-track roadway maintenance machines; general. 214.513 Section 214.513 Transportation Other Regulations Relating to... SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.513 Retrofitting of existing on...

49 CFR 214.315 - Supervision and communication.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Supervision and communication. 214.315 Section 214... Supervision and communication. (a) When an employer assigns duties to a roadway worker that call for that...: § 214.315 Supervision and communication. (a) When an employer assigns a duty to a roadway worker that...

48 CFR 1852.214-70 - Caution to offerors furnishing descriptive literature.

Code of Federal Regulations, 2013 CFR

2013-10-01

... furnishing descriptive literature. 1852.214-70 Section 1852.214-70 Federal Acquisition Regulations System... CLAUSES Texts of Provisions and Clauses 1852.214-70 Caution to offerors furnishing descriptive literature... Descriptive Literature (DEC 1988) Bidders are cautioned against furnishing as a part of their bids descriptive...

48 CFR 1852.214-70 - Caution to offerors furnishing descriptive literature.

Code of Federal Regulations, 2011 CFR

2011-10-01

... furnishing descriptive literature. 1852.214-70 Section 1852.214-70 Federal Acquisition Regulations System... CLAUSES Texts of Provisions and Clauses 1852.214-70 Caution to offerors furnishing descriptive literature... Descriptive Literature (DEC 1988) Bidders are cautioned against furnishing as a part of their bids descriptive...

48 CFR 1852.214-70 - Caution to offerors furnishing descriptive literature.

Code of Federal Regulations, 2014 CFR

2014-10-01

... furnishing descriptive literature. 1852.214-70 Section 1852.214-70 Federal Acquisition Regulations System... CLAUSES Texts of Provisions and Clauses 1852.214-70 Caution to offerors furnishing descriptive literature... Descriptive Literature (DEC 1988) Bidders are cautioned against furnishing as a part of their bids descriptive...

48 CFR 1852.214-70 - Caution to offerors furnishing descriptive literature.

Code of Federal Regulations, 2012 CFR

2012-10-01

... furnishing descriptive literature. 1852.214-70 Section 1852.214-70 Federal Acquisition Regulations System... CLAUSES Texts of Provisions and Clauses 1852.214-70 Caution to offerors furnishing descriptive literature... Descriptive Literature (DEC 1988) Bidders are cautioned against furnishing as a part of their bids descriptive...

49 CFR 214.117 - Eye and face protection.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Eye and face protection. 214.117 Section 214.117..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Bridge Worker Safety Standards § 214.117 Eye and face protection. (a) Railroad bridge workers shall be provided and shall wear eye and face protection equipment...

49 CFR 214.117 - Eye and face protection.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Eye and face protection. 214.117 Section 214.117..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Bridge Worker Safety Standards § 214.117 Eye and face protection. (a) Railroad bridge workers shall be provided and shall wear eye and face protection equipment...

49 CFR 214.117 - Eye and face protection.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Eye and face protection. 214.117 Section 214.117..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Bridge Worker Safety Standards § 214.117 Eye and face protection. (a) Railroad bridge workers shall be provided and shall wear eye and face protection equipment...

33 CFR 214.7 - Delegation of authority.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Delegation of authority. 214.7 Section 214.7 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE EMERGENCY SUPPLIES OF DRINKING WATER § 214.7 Delegation of authority. The authority to approve...

24 CFR 214.500 - Audit.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Audit. 214.500 Section 214.500... PROGRAM Other Federal Requirements § 214.500 Audit. Housing counseling grant recipients and subrecipients shall be subject to the audit requirements contained in 24 CFR parts 84 and 85. HUD must be provided a...

49 CFR 214.513 - Retrofitting of existing on-track roadway maintenance machines; general.

Code of Federal Regulations, 2011 CFR

2011-10-01

... maintenance machines; general. 214.513 Section 214.513 Transportation Other Regulations Relating to... SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.513 Retrofitting of existing on-track roadway maintenance machines; general. (a) Each existing on-track roadway maintenance machine...

49 CFR 214.515 - Overhead covers for existing on-track roadway maintenance machines.

Code of Federal Regulations, 2011 CFR

2011-10-01

... maintenance machines. 214.515 Section 214.515 Transportation Other Regulations Relating to Transportation... Roadway Maintenance Machines and Hi-Rail Vehicles § 214.515 Overhead covers for existing on-track roadway maintenance machines. (a) For those existing on-track roadway maintenance machines either currently or...

47 CFR 214.4 - Planned actions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Planned actions. 214.4 Section 214.4 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.4 Planned actions. (a) Whenever it is...

47 CFR 214.1 - Purpose.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Purpose. 214.1 Section 214.1 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.1 Purpose. The purpose of this part is to...

47 CFR 214.6 - Postattack procedures and actions.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Postattack procedures and actions. 214.6 Section 214.6 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.6 Postattack...

47 CFR 214.1 - Purpose.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Purpose. 214.1 Section 214.1 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.1 Purpose. The purpose of this part is to...

47 CFR 214.6 - Postattack procedures and actions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Postattack procedures and actions. 214.6 Section 214.6 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.6 Postattack...

47 CFR 214.4 - Planned actions.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Planned actions. 214.4 Section 214.4 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.4 Planned actions. (a) Whenever it is...

47 CFR 214.6 - Postattack procedures and actions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Postattack procedures and actions. 214.6 Section 214.6 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.6 Postattack...

47 CFR 214.1 - Purpose.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Purpose. 214.1 Section 214.1 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.1 Purpose. The purpose of this part is to...

47 CFR 214.6 - Postattack procedures and actions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Postattack procedures and actions. 214.6 Section 214.6 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.6 Postattack...

47 CFR 214.1 - Purpose.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Purpose. 214.1 Section 214.1 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.1 Purpose. The purpose of this part is to...

13 CFR 500.214 - OMB control number. [Reserved

Code of Federal Regulations, 2013 CFR

2013-01-01

... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false OMB control number. [Reserved] 500.214 Section 500.214 Business Credit and Assistance EMERGENCY OIL AND GAS GUARANTEED LOAN BOARD EMERGENCY OIL AND GAS GUARANTEED LOAN PROGRAM Oil and Gas Guaranteed Loans § 500.214 OMB control number...

49 CFR 195.214 - Welding procedures.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 3 2014-10-01 2014-10-01 false Welding procedures. 195.214 Section 195.214... PIPELINE Construction § 195.214 Welding procedures. (a) Welding must be performed by a qualified welder in accordance with welding procedures qualified under Section 5 of API 1104 or Section IX of the ASME Boiler and...

49 CFR 195.214 - Welding procedures.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 3 2011-10-01 2011-10-01 false Welding procedures. 195.214 Section 195.214... PIPELINE Construction § 195.214 Welding procedures. (a) Welding must be performed by a qualified welder in accordance with welding procedures qualified under Section 5 of API 1104 or Section IX of the ASME Boiler and...

49 CFR 195.214 - Welding procedures.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 3 2013-10-01 2013-10-01 false Welding procedures. 195.214 Section 195.214... PIPELINE Construction § 195.214 Welding procedures. (a) Welding must be performed by a qualified welder in accordance with welding procedures qualified under Section 5 of API 1104 or Section IX of the ASME Boiler and...

49 CFR 195.214 - Welding procedures.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 3 2012-10-01 2012-10-01 false Welding procedures. 195.214 Section 195.214... PIPELINE Construction § 195.214 Welding procedures. (a) Welding must be performed by a qualified welder in accordance with welding procedures qualified under Section 5 of API 1104 or Section IX of the ASME Boiler and...

47 CFR 214.4 - Planned actions.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Planned actions. 214.4 Section 214.4 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.4 Planned actions. (a) Whenever it is...

47 CFR 214.4 - Planned actions.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Planned actions. 214.4 Section 214.4 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.4 Planned actions. (a) Whenever it is...

34 CFR 685.214 - Closed school discharge.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 34 Education 4 2011-07-01 2011-07-01 false Closed school discharge. 685.214 Section 685.214... Provisions § 685.214 Closed school discharge. (a) General. (1) The Secretary discharges the borrower's (and... for which the loan was made because the school at which the borrower (or student) was enrolled closed...

12 CFR 214.5 - Accounts with foreign banks.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 2 2010-01-01 2010-01-01 false Accounts with foreign banks. 214.5 Section 214.5 Banks and Banking FEDERAL RESERVE SYSTEM BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM RELATIONS WITH FOREIGN BANKS AND BANKERS (REGULATION N) Regulations § 214.5 Accounts with foreign banks. (a...

12 CFR 214.5 - Accounts with foreign banks.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 12 Banks and Banking 2 2011-01-01 2011-01-01 false Accounts with foreign banks. 214.5 Section 214.5 Banks and Banking FEDERAL RESERVE SYSTEM BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM RELATIONS WITH FOREIGN BANKS AND BANKERS (REGULATION N) Regulations § 214.5 Accounts with foreign banks. (a...

15 CFR 280.214 - Hearings.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 15 Commerce and Foreign Trade 1 2014-01-01 2014-01-01 false Hearings. 280.214 Section 280.214... Enforcement § 280.214 Hearings. (a) Scheduling. The administrative law judge, by agreement with the parties or upon notice to all parties of not less than 30 days, will schedule a hearing. All hearings will be held...

15 CFR 280.214 - Hearings.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Hearings. 280.214 Section 280.214... Enforcement § 280.214 Hearings. (a) Scheduling. The administrative law judge, by agreement with the parties or upon notice to all parties of not less than 30 days, will schedule a hearing. All hearings will be held...

32 CFR 45.2 - Applicability and scope.

Code of Federal Regulations, 2010 CFR

2010-07-01

... CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) § 45.2 Applicability and scope. (a... on the preparation and distribution of DD Forms 214, 214WS, 215 (Appendices A, B, and C) which record... Management and Personnel) approval is obtained.) DD Forms 214 and 215 (or their substitutes) will provide: (1...

30 CFR 77.214 - Refuse piles; general.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 1 2010-07-01 2010-07-01 false Refuse piles; general. 77.214 Section 77.214... Installations § 77.214 Refuse piles; general. (a) Refuse piles constructed on or after July 1, 1971, shall be..., tipples, or other surface installations and such piles shall not be located over abandoned openings or...

36 CFR 214.8 - Appeal content.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 36 Parks, Forests, and Public Property 2 2014-07-01 2014-07-01 false Appeal content. 214.8 Section 214.8 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE POSTDECISIONAL ADMINISTRATIVE REVIEW PROCESS FOR OCCUPANCY OR USE OF NATIONAL FOREST SYSTEM LANDS AND RESOURCES § 214.8 Appeal content. (a) General requirements for...

36 CFR 214.8 - Appeal content.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false Appeal content. 214.8 Section 214.8 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF AGRICULTURE POSTDECISIONAL ADMINISTRATIVE REVIEW PROCESS FOR OCCUPANCY OR USE OF NATIONAL FOREST SYSTEM LANDS AND RESOURCES § 214.8 Appeal content. (a) General requirements for...

25 CFR 214.2 - Sale of leases.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 25 Indians 1 2014-04-01 2014-04-01 false Sale of leases. 214.2 Section 214.2 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.2 Sale of leases. Leases of minerals other than oil and gas...

25 CFR 214.2 - Sale of leases.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 25 Indians 1 2013-04-01 2013-04-01 false Sale of leases. 214.2 Section 214.2 Indians BUREAU OF INDIAN AFFAIRS, DEPARTMENT OF THE INTERIOR ENERGY AND MINERALS LEASING OF OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.2 Sale of leases. Leases of minerals other than oil and gas...

22 CFR 214.51 - Administrative review of denial for public access to records.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Administrative review of denial for public access to records. 214.51 Section 214.51 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Administrative Remedies § 214.51 Administrative review of denial for public access to...

48 CFR 1552.214-71 - Contract award-other factors-formal advertising.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contract award-other factors-formal advertising. 1552.214-71 Section 1552.214-71 Federal Acquisition Regulations System ENVIRONMENTAL PROTECTION AGENCY CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Texts of Provisions and Clauses 1552.214-71 Contract...

48 CFR 52.214-28 - Subcontractor Certified Cost or Pricing Data-Modifications-Sealed Bidding.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Cost or Pricing Data-Modifications-Sealed Bidding. 52.214-28 Section 52.214-28 Federal Acquisition... CONTRACT CLAUSES Text of Provisions and Clauses 52.214-28 Subcontractor Certified Cost or Pricing Data... Cost or Pricing Data—Modifications—Sealed Bidding (OCT 2010) (a) The requirements of paragraphs (b) and...

76 FR 58078 - Thirteenth Meeting: RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-19

... Committee 214: Working Group 78: Standards for Air Traffic Data Communication Services AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Notice of RTCA Special Committee 214: Working Group 78... public of a meeting of the RTCA Special Committee 214: Working Group 78: Standards for Air Traffic Data...

48 CFR 52.214-34 - Submission of Offers in the English Language.

Code of Federal Regulations, 2011 CFR

2011-10-01

... the English Language. 52.214-34 Section 52.214-34 Federal Acquisition Regulations System FEDERAL... Provisions and Clauses 52.214-34 Submission of Offers in the English Language. As prescribed in 14.201-6(w), insert the following provision: Submission of Offers in the English Language (APR 1991) Offers submitted...

49 CFR 214.315 - Supervision and communication.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Supervision and communication. 214.315 Section 214... Supervision and communication. Link to an amendment published at 76 FR 74614, Nov. 30, 2011. Link to a delay..., the new text is set forth as follows: § 214.315 Supervision and communication. (a) When an employer...

49 CFR 214.521 - Flagging equipment for on-track roadway maintenance machines and hi-rail vehicles.

Code of Federal Regulations, 2010 CFR

2010-10-01

... maintenance machines and hi-rail vehicles. 214.521 Section 214.521 Transportation Other Regulations Relating... WORKPLACE SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.521 Flagging equipment for on-track roadway maintenance machines and hi-rail vehicles. Each on-track roadway maintenance machine...

29 CFR 2.14 - Proceedings in which the Department balances conflicting values.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 1 2011-07-01 2011-07-01 false Proceedings in which the Department balances conflicting values. 2.14 Section 2.14 Labor Office of the Secretary of Labor GENERAL REGULATIONS Audiovisual Coverage of Administrative Hearings § 2.14 Proceedings in which the Department balances conflicting values. In...

29 CFR 2.14 - Proceedings in which the Department balances conflicting values.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 1 2010-07-01 2010-07-01 true Proceedings in which the Department balances conflicting values. 2.14 Section 2.14 Labor Office of the Secretary of Labor GENERAL REGULATIONS Audiovisual Coverage of Administrative Hearings § 2.14 Proceedings in which the Department balances conflicting values. In...

49 CFR 214.519 - Floors, decks, stairs, and ladders of on-track roadway maintenance machines.

Code of Federal Regulations, 2010 CFR

2010-10-01

... roadway maintenance machines. 214.519 Section 214.519 Transportation Other Regulations Relating to... SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.519 Floors, decks, stairs, and ladders of on-track roadway maintenance machines. Floors, decks, stairs, and ladders of on-track roadway...

49 CFR 214.519 - Floors, decks, stairs, and ladders of on-track roadway maintenance machines.

Code of Federal Regulations, 2011 CFR

2011-10-01

... roadway maintenance machines. 214.519 Section 214.519 Transportation Other Regulations Relating to... SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.519 Floors, decks, stairs, and ladders of on-track roadway maintenance machines. Floors, decks, stairs, and ladders of on-track roadway...

49 CFR 214.509 - Required visual illumination and reflective devices for new on-track roadway maintenance machines.

Code of Federal Regulations, 2011 CFR

2011-10-01

... devices for new on-track roadway maintenance machines. 214.509 Section 214.509 Transportation Other... TRANSPORTATION RAILROAD WORKPLACE SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.509 Required visual illumination and reflective devices for new on-track roadway maintenance machines. Each new...

49 CFR 214.511 - Required audible warning devices for new on-track roadway maintenance machines.

Code of Federal Regulations, 2011 CFR

2011-10-01

... roadway maintenance machines. 214.511 Section 214.511 Transportation Other Regulations Relating to... SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.511 Required audible warning devices for new on-track roadway maintenance machines. Each new on-track roadway maintenance machine shall...

49 CFR 214.511 - Required audible warning devices for new on-track roadway maintenance machines.

Code of Federal Regulations, 2010 CFR

2010-10-01

... roadway maintenance machines. 214.511 Section 214.511 Transportation Other Regulations Relating to... SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.511 Required audible warning devices for new on-track roadway maintenance machines. Each new on-track roadway maintenance machine shall...

49 CFR 214.107 - Working over or adjacent to water.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Working over or adjacent to water. 214.107 Section 214.107 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Bridge Worker Safety Standards § 214.107 Working over or adjacent to water. (a)...

49 CFR 214.107 - Working over or adjacent to water.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Working over or adjacent to water. 214.107 Section 214.107 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Bridge Worker Safety Standards § 214.107 Working over or adjacent to water. (a)...

49 CFR 214.107 - Working over or adjacent to water.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Working over or adjacent to water. 214.107 Section 214.107 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Bridge Worker Safety Standards § 214.107 Working over or adjacent to water. (a)...

22 CFR 214.42 - Uniform pay guidelines.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Uniform pay guidelines. 214.42 Section 214.42... Advisory Committees § 214.42 Uniform pay guidelines. (a) A.I.D. follows OMB/CSC guidelines in section 11 of OMB Circular A-63 in establishing rates of pay for advisory committee members, staffs, and consultants...

MicroRNA-214 protects against hypoxia/reoxygenation induced cell damage and myocardial ischemia/reperfusion injury via suppression of PTEN and Bim1 expression.

PubMed

Wang, Xiaohui; Ha, Tuanzhu; Hu, Yuanping; Lu, Chen; Liu, Li; Zhang, Xia; Kao, Race; Kalbfleisch, John; Williams, David; Li, Chuanfu

2016-12-27

Myocardial apoptosis plays an important role in myocardial ischemia/reperfusion (I/R) injury. Activation of PI3K/Akt signaling protects the myocardium from I/R injury. This study investigated the role of miR-214 in hypoxia/reoxygenation (H/R)-induced cell damage in vitro and myocardial I/R injury in vivo. H9C2 cardiomyoblasts were transfected with lentivirus expressing miR-214 (LmiR-214) or lentivirus expressing scrambled miR-control (LmiR-control) respectively, to establish cell lines of LmiR-214 and LmiR-control. The cells were subjected to hypoxia for 4 h followed by reoxygenation for 24 h. Transfection of LmiR-214 suppresses PTEN expression, significantly increases the levels of Akt phosphorylation, markedly attenuates LDH release, and enhances the viability of the cells subjected to H/R. In vivo transfection of mouse hearts with LmiR-214 significantly attenuates I/R induced cardiac dysfunction and reduces I/R-induced myocardial infarct size. LmiR-214 transfection significantly attenuates I/R-induced myocardial apoptosis and caspase-3/7 and caspase-8 activity. Increased expression of miR-214 by transfection of LmiR-214 suppresses PTEN expression, increases the levels of phosphorylated Akt, represses Bim1 expression and induces Bad phosphorylation in the myocardium. In addition, in vitro data shows transfection of miR-214 mimics to H9C2 cells suppresses the expression and translocation of Bim1 from cytosol to mitochondria and induces Bad phosphorylation. Our in vitro and in vivo data suggests that miR-214 protects cells from H/R induced damage and attenuates I/R induced myocardial injury. The mechanisms involve activation of PI3K/Akt signaling by targeting PTEN expression, induction of Bad phosphorylation, and suppression of Bim1 expression, resulting in decreases in I/R-induced myocardial apoptosis.

Role of Rhodobacter sphaeroides photosynthetic reaction center residue M214 in the composition, absorbance properties, and conformations of H(A) and B(A) cofactors.

PubMed

Saer, Rafael G; Hardjasa, Amelia; Rosell, Federico I; Mauk, A Grant; Murphy, Michael E P; Beatty, J Thomas

2013-04-02

In the native reaction center (RC) of Rhodobacter sphaeroides, the side chain of (M)L214 projects orthogonally toward the plane and into the center of the A branch bacteriopheophytin (BPhe) macrocycle. The possibility that this side chain is responsible for the dechelation of the central Mg(2+) of bacteriochlorophyll (BChl) was investigated by replacement of (M)214 with residues possessing small, nonpolar side chains that can neither coordinate nor block access to the central metal ion. The (M)L214 side chain was also replaced with Cys, Gln, and Asn to evaluate further the requirements for assembly of the RC with BChl in the HA pocket. Photoheterotrophic growth studies showed no difference in growth rates of the (M)214 nonpolar mutants at a low light intensity, but the growth of the amide-containing mutants was impaired. The absorbance spectra of purified RCs indicated that although absorbance changes are associated with the nonpolar mutations, the nonpolar mutant RC pigment compositions are the same as in the wild-type protein. Crystal structures of the (M)L214G, (M)L214A, and (M)L214N mutants were determined (determined to 2.2-2.85 Å resolution), confirming the presence of BPhe in the HA pocket and revealing alternative conformations of the phytyl tail of the accessory BChl in the BA site of these nonpolar mutants. Our results demonstrate that (i) BChl is converted to BPhe in a manner independent of the aliphatic side chain length of nonpolar residues replacing (M)214, (ii) BChl replaces BPhe if residue (M)214 has an amide-bearing side chain, (iii) (M)214 side chains containing sulfur are not sufficient to bind BChl in the HA pocket, and (iv) the (M)214 side chain influences the conformation of the phytyl tail of the BA BChl.

32 CFR Appendix B to Part 45 - DD Form 214ws

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 1 2010-07-01 2010-07-01 false DD Form 214ws B Appendix B to Part 45 National... CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) Pt. 45, App. B Appendix B to Part 45—DD Form 214ws EC23OC91.007 ...

47 CFR 25.214 - Technical requirements for space stations in the Satellite Digital Audio Radio Service and...

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 2 2014-10-01 2014-10-01 false Technical requirements for space stations in the Satellite Digital Audio Radio Service and associated terrestrial repeaters. 25.214 Section 25.214... Technical Standards § 25.214 Technical requirements for space stations in the Satellite Digital Audio Radio...

47 CFR 25.214 - Technical requirements for space stations in the satellite digital audio radio service and...

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 2 2012-10-01 2012-10-01 false Technical requirements for space stations in the satellite digital audio radio service and associated terrestrial repeaters. 25.214 Section 25.214... Technical Standards § 25.214 Technical requirements for space stations in the satellite digital audio radio...

47 CFR 25.214 - Technical requirements for space stations in the satellite digital audio radio service and...

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 2 2011-10-01 2011-10-01 false Technical requirements for space stations in the satellite digital audio radio service and associated terrestrial repeaters. 25.214 Section 25.214... Technical Standards § 25.214 Technical requirements for space stations in the satellite digital audio radio...

47 CFR 25.214 - Technical requirements for space stations in the Satellite Digital Audio Radio Service and...

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 2 2013-10-01 2013-10-01 false Technical requirements for space stations in the Satellite Digital Audio Radio Service and associated terrestrial repeaters. 25.214 Section 25.214... Technical Standards § 25.214 Technical requirements for space stations in the Satellite Digital Audio Radio...

27 CFR 555.214 - Storage within types 1, 2, 3, and 4 magazines.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 27 Alcohol, Tobacco Products and Firearms 3 2010-04-01 2010-04-01 false Storage within types 1, 2, 3, and 4 magazines. 555.214 Section 555.214 Alcohol, Tobacco Products, and Firearms BUREAU OF... Storage § 555.214 Storage within types 1, 2, 3, and 4 magazines. (a) Explosive materials within a magazine...

27 CFR 555.214 - Storage within types 1, 2, 3, and 4 magazines.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 27 Alcohol, Tobacco Products and Firearms 3 2011-04-01 2010-04-01 true Storage within types 1, 2, 3, and 4 magazines. 555.214 Section 555.214 Alcohol, Tobacco Products, and Firearms BUREAU OF... Storage § 555.214 Storage within types 1, 2, 3, and 4 magazines. (a) Explosive materials within a magazine...

47 CFR 25.214 - Technical requirements for space stations in the satellite digital audio radio service and...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 2 2010-10-01 2010-10-01 false Technical requirements for space stations in the satellite digital audio radio service and associated terrestrial repeaters. 25.214 Section 25.214... Technical Standards § 25.214 Technical requirements for space stations in the satellite digital audio radio...

43 CFR 2.14 - When can I get expedited processing?

Code of Federal Regulations, 2010 CFR

2010-10-01

... 43 Public Lands: Interior 1 2010-10-01 2010-10-01 false When can I get expedited processing? 2.14 Section 2.14 Public Lands: Interior Office of the Secretary of the Interior RECORDS AND TESTIMONY; FREEDOM OF INFORMATION ACT Requests for Records under the FOIA § 2.14 When can I get expedited processing? (a...

20 CFR 408.214 - Are you age 65?

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 2 2011-04-01 2011-04-01 false Are you age 65? 408.214 Section 408.214 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SPECIAL BENEFITS FOR CERTAIN WORLD WAR II VETERANS SVB Qualification and Entitlement Age § 408.214 Are you age 65? You become age 65 on the first moment of the day...

20 CFR 408.214 - Are you age 65?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false Are you age 65? 408.214 Section 408.214 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SPECIAL BENEFITS FOR CERTAIN WORLD WAR II VETERANS SVB Qualification and Entitlement Age § 408.214 Are you age 65? You become age 65 on the first moment of the day...

49 CFR 214.517 - Retrofitting of existing on-track roadway maintenance machines manufactured on or after January 1...

Code of Federal Regulations, 2011 CFR

2011-10-01

... maintenance machines manufactured on or after January 1, 1991. 214.517 Section 214.517 Transportation Other... TRANSPORTATION RAILROAD WORKPLACE SAFETY On-Track Roadway Maintenance Machines and Hi-Rail Vehicles § 214.517 Retrofitting of existing on-track roadway maintenance machines manufactured on or after January 1, 1991. In...

30 CFR 250.214 - What geological and geophysical (G&G) information must accompany the EP?

Code of Federal Regulations, 2011 CFR

2011-07-01

... 30 Mineral Resources 2 2011-07-01 2011-07-01 false What geological and geophysical (G&G) information must accompany the EP? 250.214 Section 250.214 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT... OUTER CONTINENTAL SHELF Plans and Information Contents of Exploration Plans (ep) § 250.214 What...

12 CFR 390.214 - How do my officers and employees file reports of personal securities trading transactions?

Code of Federal Regulations, 2013 CFR

2013-01-01

... of personal securities trading transactions? 390.214 Section 390.214 Banks and Banking FEDERAL... Transactions § 390.214 How do my officers and employees file reports of personal securities trading... person” personal securities trading report required by SEC Rule 17j-1(d), 17 CFR 270.17j-1(d). ...

Osteoclastic miR-214 targets TRAF3 to contribute to osteolytic bone metastasis of breast cancer

PubMed Central

Liu, Jin; Li, Defang; Dang, Lei; Liang, Chao; Guo, Baosheng; Lu, Cheng; He, Xiaojuan; Cheung, Hilda Y. S.; He, Bing; Liu, Biao; Li, Fangfei; Lu, Jun; Wang, Luyao; Shaikh, Atik Badshah; Jiang, Feng; Lu, Changwei; Peng, Songlin; Zhang, Zongkang; Zhang, Bao-Ting; Pan, Xiaohua; Xiao, Lianbo; Lu, Aiping; Zhang, Ge

2017-01-01

The role of osteoclastic miRNAs in regulating osteolytic bone metastasis (OBM) of breast cancer is still underexplored. Here, we examined the expression profiles of osteoclastogenic miRNAs in human bone specimens and identified that miR-214-3p was significantly upregulated in breast cancer patients with OBM. Consistently, we found increased miR-214-3p within osteoclasts, which was associated with the elevated bone resorption, during the development of OBM in human breast cancer xenografted nude mice (BCX). Furthermore, genetic ablation of osteoclastic miR-214-3p in nude mice prevent the development of OBM. Conditioned medium from MDA-MB-231 cells dramatically stimulated miR-214-3p expression to promote osteoclast differentiation. Mechanistically, a series of in vitro study showed that miR-214-3p directly targeted Traf3 to promote osteoclast activity and bone-resorbing activity. In addition, osteoclast-specific miR-214-3p knock-in mice showed remarkably increased bone resorption when compared to the littermate controls, which was attenuated after osteoclast-targeted treatment with Traf3 3′UTR-containing plasmid. In BCX nude mice, osteoclast-targeted antagomir-214-3p delivery could recover the TRAF3 protein expression and attenuate the development of OBM, respectively. Collectively, inhibition of osteoclastic miR-214-3p may be a potential therapeutic strategy for breast cancer patients with OBM. Meanwhile, the intraosseous TRAF3 could be a promising biomarker for evaluation of the treatment response of antagomir-214-3p. PMID:28071724

Anti-fibrotic Role of miR-214 in Thioacetamide-induced Liver Cirrhosis in Rats.

PubMed

Izawa, Takeshi; Horiuchi, Takashi; Atarashi, Machi; Kuwamura, Mitsuru; Yamate, Jyoji

2015-08-01

An increasing number of studies have focused on the role of microRNAs in liver fibrosis/cirrhosis. miR-214 has recently attracted more attention as a fibrosis-related factor; however, the molecular mechanisms in hepatic fibrogenesis remain largely unknown. Here, we investigate the pathological role of miR-214 during progression of liver cirrhosis in rats. Rats were injected intraperitoneally with thioacetamide at a dose of 100 mg/kg body weight, twice a week. The liver was collected at post first injection weeks 5, 10, 15, and 20. Hepatic expression of miR-214 was analyzed by real-time polymerase chain reaction, in situ hybridization, and laser microdissection. The effects of miR-214 overexpression were investigated by in vitro transfection using fibroblastic MT-9 cells. miR-214 was highly upregulated in the fibrotic area in parallel with the cirrhosis progression. miR-214 overexpression in MT-9 cells under transforming growth factor-β1 stimulation resulted in decreased cell number and increased expression of cleaved caspase 3 and decreased expression of α-smooth muscle actin, suggesting that miR-214 induces apoptosis and inhibits myofibroblast differentiation in fibroblastic cells under stimulation of fibrogenic factors. These data indicate an anti-fibrotic role of miR-214 in chemically induced liver fibrosis/cirrhosis. © 2015 by The Author(s).

75 FR 55272 - Safety Zone; Mississippi River, Mile 212.0 to 214.5

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-10

...-AA00 Safety Zone; Mississippi River, Mile 212.0 to 214.5 AGENCY: Coast Guard, DHS. ACTION: Temporary... Mississippi River, Mile 212.0 to 214.5, extending West of Portage Island to the right descending bank of the... based fireworks display between mile 212.0 to 214.5 on the Upper Mississippi River. This event presents...

20 CFR 408.214 - Are you age 65?

Code of Federal Regulations, 2012 CFR

2012-04-01

... 20 Employees' Benefits 2 2012-04-01 2012-04-01 false Are you age 65? 408.214 Section 408.214... Qualification and Entitlement Age § 408.214 Are you age 65? You become age 65 on the first moment of the day before the anniversary of your birth corresponding to age 65. Thus, you must have been born on or before...

20 CFR 408.214 - Are you age 65?

Code of Federal Regulations, 2013 CFR

2013-04-01

... 20 Employees' Benefits 2 2013-04-01 2013-04-01 false Are you age 65? 408.214 Section 408.214... Qualification and Entitlement Age § 408.214 Are you age 65? You become age 65 on the first moment of the day before the anniversary of your birth corresponding to age 65. Thus, you must have been born on or before...

20 CFR 408.214 - Are you age 65?

Code of Federal Regulations, 2014 CFR

2014-04-01

... 20 Employees' Benefits 2 2014-04-01 2014-04-01 false Are you age 65? 408.214 Section 408.214... Qualification and Entitlement Age § 408.214 Are you age 65? You become age 65 on the first moment of the day before the anniversary of your birth corresponding to age 65. Thus, you must have been born on or before...

12 CFR 741.214 - Report of crime or catastrophic act and Bank Secrecy Act compliance.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Report of crime or catastrophic act and Bank Secrecy Act compliance. 741.214 Section 741.214 Banks and Banking NATIONAL CREDIT UNION ADMINISTRATION... Unions § 741.214 Report of crime or catastrophic act and Bank Secrecy Act compliance. Any credit union...

44Sc-DOTA-BN[2-14]NH2 in comparison to 68Ga-DOTA-BN[2-14]NH2 in pre-clinical investigation. Is 44Sc a potential radionuclide for PET?

PubMed

Koumarianou, E; Loktionova, N S; Fellner, M; Roesch, F; Thews, O; Pawlak, D; Archimandritis, S C; Mikolajczak, R

2012-12-01

In the present study we demonstrate the in vitro and in vivo comparison of the (44)Sc and (68)Ga labeled DOTA-BN[2-14]NH(2). (44)Sc is a positron emitter with a half life of 3.92 h. Hence it could be used for PET imaging with ligands requiring longer observation time than in the case of (68)Ga. The binding affinity of (nat)Sc-DOTA-BN[2-14]NH(2) and (nat)Ga-DOTA-BN[2-14]NH(2) to GRP receptors was studied in competition to [(125)I-Tyr(4)]-Bombesin in the human prostate cancer cell line PC-3. A preliminary biodistribution in normal rats was performed, while first microPET images were assessed in male Copenhagen rats bearing the androgen-independent Dunning R-3327-AT-1 prostate cancer tumor. The affinity to GRP receptors in the PC-3 cell line was higher for (nat)Ga-DOTA-BN[2-14]NH(2) (IC(50)(nM)=0.85 ± 0.06) than that of (nat)Sc-DOTA-BN[2-14]NH(2) (IC(50) (nM)=6.49 ± 0.13). The internalization rate of (68)Ga labeled DOTA-BN[2-14]NH(2) was slower than that of (44)Sc, but their final internalization percents were comparable. (68)Ga-DOTA-BN[2-14]NH(2) was externalized faster than (44)Sc-DOTA-BN[2-14]NH(2). The biodistribution of (44)Sc-DOTA-BN[2-14]NH(2) and (68)Ga-DOTA-BN[2-14]NH(2) in normal rats revealed a higher uptake in target organs and tissues of the first one while both excreted mainly through urinary tract. In microPET images both tracers were accumulated in the tumor with similar uptake patterns. Despite the differences in the receptor affinity both the (68)Ga- and the (44)Sc-labeled DOTA-BN[2-14]NH(2) tracers showed comparable distribution and similar time constants of uptake and elimination. Moreover no differences in tumor accumulation (neither in the overall uptake nor in the dynamics) were observed from the microPet imaging. From that perspective the use of either (44)Sc or (68)Ga for detecting tumors with GRP receptors is equivalent. Copyright © 2012 Elsevier Ltd. All rights reserved.

Inhibitors of Eicosanoid Biosynthesis Influencing the Transcripts Level of sHSP21.4 Gene Induced by Pathogen Infections, in Antheraea pernyi

PubMed Central

Zhang, Congfen; Dai, Lishang; Wang, Lei; Qian, Cen; Wei, Guoqing; Li, Jun; Zhu, Baojian; Liu, Chaoliang

2015-01-01

Small heat shock proteins (sHSPs) can regulate protein folding and protect cells from stress. To investigate the role of sHSPs in the silk-producing insect Antheraea pernyi response to microorganisms, a sHsp gene termed as Ap-sHSP21.4, was identified. This gene encoded a 21.4 kDa protein which shares the conserved structure of insect sHsps and belongs to sHSP21.4 family. Ap-sHSP21.4 was highly expressed in fat body and up-regulated in midgut and fat body of A. pernyi challenged with Escherichia coli, Beauveria bassiana and nuclear polyhedrosis virus (NPV), which was determined by quantitative real-time PCR. Meanwhile, knock down of Ap-sHSP21.4 with dsRNA result in the decrease at the expression levels of several immune response-related genes (defensin, Dopa decarboxylase, Toll1, lysozyme and Kazal-type serine protease inhibitor). Additionally, the impact of eicosanoid biosynthesis on the expression of Ap-sHSP21.4 response to NPV was determined using qPCR, inhibitors of eicosanoid biosynthesis significantly suppress Ap-HSP21.4 expression upon NPV challenge. All together, Ap-sHSP21.4 was involved in the immunity of A. pernyi against microorganism and possibly mediated by eicosanoids pathway. These results will shed light in the understanding of the pathogen-host interaction in A. pernyi. PMID:25844646

miR-214 down-regulates ARL2 and suppresses growth and invasion of cervical cancer cells.

PubMed

Peng, Ruiqing; Men, Jianlong; Ma, Rui; Wang, Qian; Wang, Yang; Sun, Ying; Ren, Jing

2017-03-11

Increasing evidence has shown that miRNAs are implicated in carcinogenesis and can function as oncogenes or tumor suppressor genes in human cancers. In this study, we confirmed that miR-214 is frequently down-regulated in cervical cancer compared with normal cervical tissues. Ectopic expression of miR-214 suppressed proliferation, migration and invasion of HeLa and C33A cervical cancer cells. Bioinformatics analysis revealed that ADP ribosylation factor like 2 (ARL2) was a potential target of miR-214 and was remarkably up-regulated in cervical cancer. Knockdown of ARL2 markedly inhibited cervical cancer cell proliferation, migration and invasion, similarly to over-expression of miR-214, indicating that ARL2 may function as an oncogene in cervical cancer. In conclusion, our study revealed that miR-214 acts as a tumor suppressor via inhibiting proliferation, migration and invasion of cervical cancer cells through targeting ARL2, and that both miR-214 and ARL2 may serve as prognostic or therapeutic targets for cervical cancer. Copyright © 2017 Elsevier Inc. All rights reserved.

Tumor-secreted miR-214 induces regulatory T cells: a major link between immune evasion and tumor growth

PubMed Central

Yin, Yuan; Cai, Xing; Chen, Xi; Liang, Hongwei; Zhang, Yujing; Li, Jing; Wang, Zuoyun; Chen, Xiulan; Zhang, Wen; Yokoyama, Seiji; Wang, Cheng; Li, Liang; Li, Limin; Hou, Dongxia; Dong, Lei; Xu, Tao; Hiroi, Takachika; Yang, Fuquan; Ji, Hongbin; Zhang, Junfeng; Zen, Ke; Zhang, Chen-Yu

2014-01-01

An increased population of CD4+CD25highFoxp3+ regulatory T cells (Tregs) in the tumor-associated microenvironment plays an important role in cancer immune evasion. However, the underlying mechanism remains unclear. Here we observed an increased secretion of miR-214 in various types of human cancers and mouse tumor models. Tumor-secreted miR-214 was sufficiently delivered into recipient T cells by microvesicles (MVs). In targeted mouse peripheral CD4+ T cells, tumor-derived miR-214 efficiently downregulated phosphatase and tensin homolog (PTEN) and promoted Treg expansion. The miR-214-induced Tregs secreted higher levels of IL-10 and promoted tumor growth in nude mice. Furthermore, in vivo studies indicated that Treg expansion mediated by cancer cell-secreted miR-214 resulted in enhanced immune suppression and tumor implantation/growth in mice. The MV delivery of anti-miR-214 antisense oligonucleotides (ASOs) into mice implanted with tumors blocked Treg expansion and tumor growth. Our study reveals a novel mechanism through which cancer cell actively manipulates immune response via promoting Treg expansion. PMID:25223704

32 CFR Appendix A to Part 45 - DD Form 214

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 1 2010-07-01 2010-07-01 false DD Form 214 A Appendix A to Part 45 National... CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) Pt. 45, App. A Appendix A to Part 45—DD Form 214 EC23OC91.003 EC23OC91.004 EC23OC91.005 EC23OC91.006 [54 FR 9985, Mar. 9, 1989] ...

Credit WCT. Original 21/4"x21/4" color negative is housed in the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Credit WCT. Original 2-1/4"x2-1/4" color negative is housed in the JPL Photography Laboratory, Pasadena, California. JPL staff member Leonard "Dutch" Sebring loads propellant grain into tube for a BATES (Ballistic And Test Evaluation System) test (JPL negative no. JPL-10279BC, 27 January 1989) - Jet Propulsion Laboratory Edwards Facility, Weigh & Test Preparation Building, Edwards Air Force Base, Boron, Kern County, CA

MiR-214 inhibits cell migration, invasion and promotes the drug sensitivity in human cervical cancer by targeting FOXM1.

PubMed

Wang, Jian-Mei; Ju, Bao-Hui; Pan, Cai-Jun; Gu, Yan; Li, Meng-Qi; Sun, Li; Xu, Yan-Ying; Yin, Li-Rong

2017-01-01

MicroRNAs (miRNAs) play key roles in progression of cervical cancer. In the present study, we investigated the role of miR-214 in the process of migration, invasion and drug sensitivity to cisplatin in cervical cancer. We detected the differential expression of miR-214 in 19 cases cervical cancer tissues and normal tissues as well as 4 cervical cancer cells and one normal cervical cells by Real-time PCR. Then, wound healing assay, transwell invasion assay and MTT were used to detect the effects of migration, invasion and sensitivity to cisplatin of cervical cancer when miR-214 was overexpressed. Western blot, immunofluorescence and Flow Cytometry were used to detect the mechanism of migration, invasion and sensitivity to cisplatin. Next, bioinformatics analysis was used to find the target of miR-214. Through the luciferase reporter assay, Real-time PCR and western blot, we confirmed the binding relationship of miR-214 and FOXM1. In cervical cancer tissues, the expression of FOXM1 was detected by western blot and Immunohistochemistry. We also knocked down FOXM1 in cervical cancer cells, wound healing assay, transwell invasion assay and MTT were performed to detect the migration, invasion and sensitivity to cisplatin abilities of FOXM1. Western blot and Flow Cytometry were used to detect the mechanism of migration, invasion and sensitivity to cisplatin by FOXM1. Finally, we performed rescue expriments to confirm the function relationship between miR-214 and FOXM1. 1. Our results showed that miR-214 was frequently downregulated in tumor tissues and cancer cells especially in CIN III and cervical cancer stages. 2. Overexpression of miR-214 significantly inhibited migration and invasion of cervical cancer cells and prompted the sensitivity to cisplatin. 3. FOXM1 was identified as a target of miR-214 and down-regulated by miR-214. 4. Knocking down FOXM1 could inhibited migration and invasion of cervical cancer cells and prompted the sensitivity to cisplatin. 5. FOXM1 was upregulated in tumor tissues. 6. The mechanism of migration, invasion and sensitivity to cisplatin were the resluts of changes of EMT and apoptosis. 7. The restoration of FOXM1 expression can counteract the effect of miR-214 on cell migration, invasion and sensitivity to cisplatin of cervical cancer cells. These findings indicate that miR-214 acts as a tumor suppressor during the process of migration, invasion and drug sensitivity through targeting FOXM1, suggesting miR-214 as a potential new diagnostic and therapeutic target for the treatment of cervical cancer.

Inhibition of microRNA-214-5p promotes cell survival and extracellular matrix formation by targeting collagen type IV alpha 1 in osteoblastic MC3T3-E1 cells.

PubMed

Li, Q S; Meng, F Y; Zhao, Y H; Jin, C L; Tian, J; Yi, X J

2017-08-01

This study aimed to investigate the functional effects of microRNA (miR)-214-5p on osteoblastic cells, which might provide a potential role of miR-214-5p in bone fracture healing. Blood samples were obtained from patients with hand fracture or intra-articular calcaneal fracture and from healthy controls (HCs). Expression of miR-214-5p was monitored by qRT-PCR at day 7, 14 and 21 post-surgery. Mouse osteoblastic MC3T3-E1 cells were transfected with antisense oligonucleotides (ASO)-miR-214-5p, collagen type IV alpha 1 (COL4A1) vector or their controls; thereafter, cell viability, apoptotic rate, and the expression of collagen type I alpha 1 (COL1A1), type II collagen (COL-II), and type X collagen (COL-X) were determined. Luciferase reporter assay, qRT-PCR, and Western blot were performed to ascertain whether COL4A1 was a target of miR-214-5p. Plasma miR-214-5p was highly expressed in patients with bone fracture compared with HCs after fracture (p < 0.05 or p < 0.01). Inhibition of miR-214-5p increased the viability of MC3T3-E1 cells and the expressions of COL1A1 and COL-X, but decreased the apoptotic rate and COL-II expression (p < 0.05 or p < 0.01). COL4A1 was a target of miR-214-5p, and was negatively regulated by miR-214-5p (p < 0.05 or p < 0.01). Overexpression of COL4A1 showed a similar impact on cell viability, apoptotic rate, and COL1A1, COL-II, and COL-X expressions inhibiting miR-214-5p (p < 0.01). Inhibition of miR-214-5p promotes cell survival and extracellular matrix (ECM) formation of osteoblastic MC3T3-E1 cells by targeting COL4A1. Cite this article: Q. S. Li, F. Y. Meng, Y. H. Zhao, C. L. Jin, J. Tian, X. J. Yi. Inhibition of microRNA-214-5p promotes cell survival and extracellular matrix formation by targeting collagen type IV alpha 1 in osteoblastic MC3T3-E1 cells. Bone Joint Res 2017;6:464-471. DOI: 10.1302/2046-3758.68.BJR-2016-0208.R2. © 2017 Yi et al.

49 CFR 214.115 - Foot protection.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Foot protection. 214.115 Section 214.115 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION... conform to the national consensus standards for safety-toe footwear (American National Standards Institute...

49 CFR 214.113 - Head protection.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Head protection. 214.113 Section 214.113 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION... conform to the national consensus standards for industrial head protection (American National Standards...

10 CFR 1021.214 - Adjudicatory proceedings.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 10 Energy 4 2010-01-01 2010-01-01 false Adjudicatory proceedings. 1021.214 Section 1021.214 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NATIONAL ENVIRONMENTAL POLICY ACT IMPLEMENTING PROCEDURES DOE... DOE adjudicatory proceedings, excluding judicial or administrative civil or criminal enforcement...

Down-regulation of long non-coding RNA RP11-708H21.4 is associated with poor prognosis for colorectal cancer and promotes tumorigenesis through regulating AKT/mTOR pathway.

PubMed

Sun, Longci; Jiang, Chunhui; Xu, Chunjie; Xue, Hanbing; Zhou, Hong; Gu, Lei; Liu, Ye; Xu, Qing

2017-04-25

Long non-coding RNAs (lncRNAs) serve critical roles in cancer development and progression. Herein, through next generation RNA sequencing and experimental validations, we determined the expression status of RP11-708H21.4 in colorectal cancer (CRC) and explored its clinical significance and biological functions in CRC. Differentially expressed lncRNAs from CRC samples and corresponding normal mucosa tissues was screened through RNA sequencing, and RP11-708H21.4 was selected for further experimental validation. The expression levels of RP11-708H21.4 in CRC tissues and cell lines were determined using qRT-PCR. Also, the relationship between the clinicopathological features and RP11-708H21.4 expression was analyzed. Cell viability was examined by CCK-8 and colony assays; cell migration and invasion were detected by transwell assays; cell cycle and cell apoptosis were analyzed by flow cytometry. The chemosensitivity of CRC cells to 5-Fluorouracil (5-FU) was also determined using CCK-8 assay. CRC xenograft tumor models were established to determine the biological functions of RP11-708H21.4 in vivo. Levels of cell cycle-related proteins and AKT/mTOR pathway-related proteins were detected by western blot assay. RP11-708H21.4 expression was aberrantly decreased in CRC, and its expression was closely associated with aggressive clinicopathologic features and unfavorable prognosis of CRC patients. Overexpressed RP11-708H21.4 suppresses CRC cell proliferation through inducing G1 arrest. Moreover, up-regulation of RP11-708H21.4 inhibits cell migration and invasion, causes cell apoptosis, and enhances 5-FU sensitivity of CRC cells. Finally, increased RP11-708H21.4 expression blocked AKT/mTOR pathway, and repressed in vivo CRC xenograft tumor growth. The results indicated that RP11-708H21.4 might have potential roles as a biomarker and a therapeutic target for CRC.

29 CFR 1603.214 - Evidence.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 4 2010-07-01 2010-07-01 false Evidence. 1603.214 Section 1603.214 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES FOR PREVIOUSLY EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION UNDER SECTION 304 OF THE...

29 CFR 1603.214 - Evidence.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 4 2011-07-01 2011-07-01 false Evidence. 1603.214 Section 1603.214 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES FOR PREVIOUSLY EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION UNDER SECTION 304 OF THE...

29 CFR 1603.214 - Evidence.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 4 2012-07-01 2012-07-01 false Evidence. 1603.214 Section 1603.214 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES FOR PREVIOUSLY EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION UNDER SECTION 304 OF THE...

29 CFR 1603.214 - Evidence.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 4 2013-07-01 2013-07-01 false Evidence. 1603.214 Section 1603.214 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES FOR PREVIOUSLY EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION UNDER SECTION 304 OF THE...

29 CFR 1603.214 - Evidence.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 4 2014-07-01 2014-07-01 false Evidence. 1603.214 Section 1603.214 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES FOR PREVIOUSLY EXEMPT STATE AND LOCAL GOVERNMENT EMPLOYEE COMPLAINTS OF EMPLOYMENT DISCRIMINATION UNDER SECTION 304 OF THE...

23 CFR 971.214 - Federal lands congestion management system (CMS).

Code of Federal Regulations, 2010 CFR

2010-04-01

...) Traffic operational improvements; (C) Public transportation improvements; (D) ITS technologies; and (E....214 Section 971.214 Highways FEDERAL HIGHWAY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL... level at which transportation system performance is no longer acceptable due to traffic interference...

22 CFR 214.34 - Public participation.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Public participation. 214.34 Section 214.34 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Operation of Advisory... AID Advisory Committee Representative, through the Advisory Committee Management Officer and the...

24 CFR 214.311 - Funding.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Funding. 214.311 Section 214.311 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

24 CFR 214.311 - Funding.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Funding. 214.311 Section 214.311 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

24 CFR 214.311 - Funding.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Funding. 214.311 Section 214.311 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued) OFFICE OF ASSISTANT SECRETARY FOR HOUSING-FEDERAL HOUSING COMMISSIONER, DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT...

49 CFR 384.214 - Reciprocity.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 5 2012-10-01 2012-10-01 false Reciprocity. 384.214 Section 384.214 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY... Reciprocity. The State must allow any person to operate a CMV in the State who is not disqualified from...

49 CFR 384.214 - Reciprocity.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 5 2014-10-01 2014-10-01 false Reciprocity. 384.214 Section 384.214 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY... Reciprocity. The State must allow any person to operate a CMV in the State who is not disqualified from...

49 CFR 384.214 - Reciprocity.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 5 2011-10-01 2011-10-01 false Reciprocity. 384.214 Section 384.214 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY... Reciprocity. The State must allow any person to operate a CMV in the State who is not disqualified from...

49 CFR 384.214 - Reciprocity.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 5 2013-10-01 2013-10-01 false Reciprocity. 384.214 Section 384.214 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY... Reciprocity. The State must allow any person to operate a CMV in the State who is not disqualified from...

49 CFR 384.214 - Reciprocity.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 5 2010-10-01 2010-10-01 false Reciprocity. 384.214 Section 384.214 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY... Reciprocity. The State shall allow any person to operate a CMV in the State who is not disqualified from...

24 CFR 214.300 - Counseling services.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Counseling services. 214.300... HOUSING COUNSELING PROGRAM Program Administration § 214.300 Counseling services. (a) Basic requirements. (1) Agencies must provide counseling to current and potential homeowners and tenants to assist them...

24 CFR 214.300 - Counseling services.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Counseling services. 214.300... HOUSING COUNSELING PROGRAM Program Administration § 214.300 Counseling services. (a) Basic requirements. (1) Agencies must provide counseling to current and potential homeowners and tenants to assist them...

24 CFR 214.300 - Counseling services.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Counseling services. 214.300... HOUSING COUNSELING PROGRAM Program Administration § 214.300 Counseling services. (a) Basic requirements. (1) Agencies must provide counseling to current and potential homeowners and tenants to assist them...

24 CFR 214.300 - Counseling services.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Counseling services. 214.300... HOUSING COUNSELING PROGRAM Program Administration § 214.300 Counseling services. (a) Basic requirements. (1) Agencies must provide counseling to current and potential homeowners and tenants to assist them...

22 CFR 214.14 - Charter revision.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Charter revision. 214.14 Section 214.14 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Establishment of Advisory...) Charter revision requires clearances by the advisory committee, the A.I.D. Advisory Committee Management...

49 CFR 214.315 - Supervision and communication.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Supervision and communication. 214.315 Section 214.315 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD... Supervision and communication. (a) When an employer assigns duties to a roadway worker that call for that...

7 CFR 1260.214 - Personal liability.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 10 2010-01-01 2010-01-01 false Personal liability. 1260.214 Section 1260.214 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... behalf of the Board, shall be held personally responsible, either individually or jointly, in any way...

32 CFR 552.214 - Procedures.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 32 National Defense 3 2012-07-01 2009-07-01 true Procedures. 552.214 Section 552.214 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY MILITARY RESERVATIONS AND NATIONAL... services to the participants. (b) Based on the Commander's concerns for discipline, mission accomplishment...

32 CFR 552.214 - Procedures.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 32 National Defense 3 2014-07-01 2014-07-01 false Procedures. 552.214 Section 552.214 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY MILITARY RESERVATIONS AND NATIONAL... services to the participants. (b) Based on the Commander's concerns for discipline, mission accomplishment...

32 CFR 552.214 - Procedures.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 3 2011-07-01 2009-07-01 true Procedures. 552.214 Section 552.214 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY MILITARY RESERVATIONS AND NATIONAL... services to the participants. (b) Based on the Commander's concerns for discipline, mission accomplishment...

32 CFR 552.214 - Procedures.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 32 National Defense 3 2010-07-01 2010-07-01 true Procedures. 552.214 Section 552.214 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY MILITARY RESERVATIONS AND NATIONAL... services to the participants. (b) Based on the Commander's concerns for discipline, mission accomplishment...

32 CFR 552.214 - Procedures.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 3 2013-07-01 2013-07-01 false Procedures. 552.214 Section 552.214 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY MILITARY RESERVATIONS AND NATIONAL... services to the participants. (b) Based on the Commander's concerns for discipline, mission accomplishment...

Credit WCT. Original 21/4"x21/4" color negative is housed in the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Credit WCT. Original 2-1/4"x2-1/4" color negative is housed in the JPL Photography Laboratory, Pasadena, California. This interior view of the Xray chamber shows operator Leonard "Dutch" Sebring positioning the 1 million electron volt X-ray machine to make an image of a Syncom 2 motor (JPL negative no. JPL-10285BC, 27 January 1989) - Jet Propulsion Laboratory Edwards Facility, Radiographic Inspection Building, Edwards Air Force Base, Boron, Kern County, CA

Credit WCT. Original 21/4"x21/4" color negative is housed in the ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Credit WCT. Original 2-1/4"x2-1/4" color negative is housed in the JPL Photography Laboratory, Pasadena, California. At one time, Building 4285/E-86 accommodated tensile testing of propellant samples. This view shows a tensile strength tester set up for propellant tests, under the supervision of JPL staff member Milton Clay (JPL negative no. JPL-10291AC, 27 January 1989) - Jet Propulsion Laboratory Edwards Facility, Casting & Curing Building, Edwards Air Force Base, Boron, Kern County, CA

78 FR 4155 - Agency Information Collection Activities: Application for Foreign Trade Zone and/or Status...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-18

...As part of its continuing effort to reduce paperwork and respondent burden, CBP invites the general public and other Federal agencies to comment on an information collection requirement concerning the Application for Foreign Trade Zone Admission and/or Status Designation, and Application for Foreign Trade Zone Activity Permit (CBP Forms 214, 214A, 214B, 214C and 216). This request for comment is being made pursuant to the Paperwork Reduction Act of 1995 (Pub. L. 104-13).

Involvement of ectodomain Leu 214 in ATP binding and channel desensitization of the P2X4 receptor.

PubMed

Zhang, Longmei; Xu, Huijuan; Jie, Yanling; Gao, Chao; Chen, Wanjuan; Yin, Shikui; Samways, Damien S K; Li, Zhiyuan

2014-05-13

P2X receptors are trimeric ATP-gated cation permeable ion channels. When ATP binds, the extracellular head and dorsal fin domains are predicted to move closer to each other. However, there are scant functional data corroborating the role of the dorsal fin in ligand binding. Here using site-directed mutagenesis and electrophysiology, we show that a dorsal fin leucine, L214, contributes to ATP binding. Mutant receptors containing a single substitution of alanine, serine, glutamic acid, or phenylalanine at L214 of the rat P2X4 receptor exhibited markedly reduced sensitivities to ATP. Mutation of other dorsal fin side chains, S216, T223, and D224, did not significantly alter ATP sensitivity. Exposure of L214C to sodium (2-sulfonatoethyl) methanethiosulfonate (MTSES(-)) or (2-aminoethyl) methanethiosulfonate hydrobromide in the absence of ATP blocked responses evoked by subsequent ATP application. In contrast, when MTSES(-) was applied in the presence of ATP, no current inhibition was observed. Furthermore, L214A also slightly reduced the inhibitory effect of the antagonist 2',3'-O-(2,4,6-trinitrophenyl)-ATP, and the blockade was more rapidly reversible after washout. Certain L214 mutants also showed effects on current desensitization in the continued presence of ATP. L214I exhibited an accelerated current decline, whereas L214M exhibited a slower rate. Taken together, these data reveal that position L214 participates in both ATP binding and conformational changes accompanying channel opening and desensitization, providing compelling evidence that the dorsal fin domain indeed has functional properties that are similar to those previously reported for the body domains.

48 CFR 833.214 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2011 CFR

2011-10-01

... resolution (ADR). 833.214 Section 833.214 Federal Acquisition Regulations System DEPARTMENT OF VETERANS... Alternative dispute resolution (ADR). Contracting officers and contractors are encouraged to use alternative dispute resolution (ADR) procedures. CBCA guidance on ADR may be obtained at http://www.cbca.gsa.gov. ...

48 CFR 33.214 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2011 CFR

2011-10-01

... resolution (ADR). 33.214 Section 33.214 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... dispute resolution (ADR). (a) The objective of using ADR procedures is to increase the opportunity for relatively inexpensive and expeditious resolution of issues in controversy. Essential elements of ADR include...

48 CFR 33.214 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2010 CFR

2010-10-01

... resolution (ADR). 33.214 Section 33.214 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... dispute resolution (ADR). (a) The objective of using ADR procedures is to increase the opportunity for relatively inexpensive and expeditious resolution of issues in controversy. Essential elements of ADR include...

48 CFR 833.214 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2010 CFR

2010-10-01

... resolution (ADR). 833.214 Section 833.214 Federal Acquisition Regulations System DEPARTMENT OF VETERANS... Alternative dispute resolution (ADR). Contracting officers and contractors are encouraged to use alternative dispute resolution (ADR) procedures. CBCA guidance on ADR may be obtained at http://www.cbca.gsa.gov. ...

24 CFR 214.200 - Inactive status.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Inactive status. 214.200 Section 214.200 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES HOUSING...

22 CFR 214.12 - Considerations in membership selection.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Considerations in membership selection. 214.12... Establishment of Advisory Committees § 214.12 Considerations in membership selection. Membership is to be fairly... also given to factors of sex, race, creed, national origin, and religion. ...

22 CFR 214.12 - Considerations in membership selection.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Considerations in membership selection. 214.12... Establishment of Advisory Committees § 214.12 Considerations in membership selection. Membership is to be fairly... also given to factors of sex, race, creed, national origin, and religion. ...

22 CFR 214.12 - Considerations in membership selection.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Considerations in membership selection. 214.12... Establishment of Advisory Committees § 214.12 Considerations in membership selection. Membership is to be fairly... also given to factors of sex, race, creed, national origin, and religion. ...

22 CFR 214.12 - Considerations in membership selection.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Considerations in membership selection. 214.12... Establishment of Advisory Committees § 214.12 Considerations in membership selection. Membership is to be fairly... also given to factors of sex, race, creed, national origin, and religion. ...

22 CFR 214.36 - Records of advisory committees.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Records of advisory committees. 214.36 Section 214.36 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Operation... Committee Management Officer. (b) Such records are to include the reports, transcripts, minutes, appendices...

22 CFR 214.37 - Public access to committee records.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Section 214.37 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Operation of Advisory Committees § 214.37 Public access to committee records. Records maintained in... 212), subject to the general oversight of the A.I.D. Advisory Committee Management Officer. (Sec. 621...

22 CFR 214.22 - Responsibilities within A.I.D.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Responsibilities within A.I.D. 214.22 Section 214.22 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT... the advisory committee reports: prepares, clears with the Advisory Committee Management Officer and...

22 CFR 214.44 - Annual review and reports.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Annual review and reports. 214.44 Section 214.44 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT... by GSA. (d) Within A.I.D., the Advisory Committee Management Officer collects required information...

4 CFR 21.4 - Protective orders.

Code of Federal Regulations, 2010 CFR

2010-01-01

... the applicant is not involved in competitive decision-making for any firm that could gain a... 4 Accounts 1 2010-01-01 2010-01-01 false Protective orders. 21.4 Section 21.4 Accounts GOVERNMENT... protected information. Such information may include proprietary, confidential, or source-selection-sensitive...

34 CFR 668.214 - Participation rate index appeals.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false Participation rate index appeals. 668.214 Section 668.214 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION STUDENT ASSISTANCE GENERAL PROVISIONS Cohort Default Rates...

40 CFR 94.214 - Production engines.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 40 Protection of Environment 20 2010-07-01 2010-07-01 false Production engines. 94.214 Section 94...) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Certification Provisions § 94.214 Production engines. Any manufacturer obtaining certification under this part shall supply to the Administrator, upon...

48 CFR 214.407-3 - Other mistakes disclosed before award.

Code of Federal Regulations, 2010 CFR

2010-10-01

...) Missile Defense Agency: General Counsel, MDA. (ix) Defense Contract Management Agency: General Counsel... before award. 214.407-3 Section 214.407-3 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SEALED BIDDING Opening of...

49 CFR 214.315 - Supervision and communication.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Supervision and communication. 214.315 Section 214.315 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD... Supervision and communication. (a) When an employer assigns a duty to a roadway worker that calls for that...

47 CFR 214.5 - Responsibilities.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Responsibilities. 214.5 Section 214.5 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND..., will issue such policy guidance, rules, regulations, procedures, and directives as may be necessary to...

47 CFR 214.5 - Responsibilities.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Responsibilities. 214.5 Section 214.5 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND..., will issue such policy guidance, rules, regulations, procedures, and directives as may be necessary to...

47 CFR 214.5 - Responsibilities.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 5 2010-10-01 2010-10-01 false Responsibilities. 214.5 Section 214.5 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND..., will issue such policy guidance, rules, regulations, procedures, and directives as may be necessary to...

47 CFR 214.5 - Responsibilities.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Responsibilities. 214.5 Section 214.5 Telecommunication OFFICE OF SCIENCE AND TECHNOLOGY POLICY AND NATIONAL SECURITY COUNCIL PROCEDURES FOR THE USE AND..., will issue such policy guidance, rules, regulations, procedures, and directives as may be necessary to...

40 CFR 94.214 - Production engines.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 40 Protection of Environment 21 2012-07-01 2012-07-01 false Production engines. 94.214 Section 94...) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Certification Provisions § 94.214 Production engines. Any manufacturer obtaining certification under this part shall supply to the Administrator, upon...

40 CFR 94.214 - Production engines.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 40 Protection of Environment 20 2014-07-01 2013-07-01 true Production engines. 94.214 Section 94...) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Certification Provisions § 94.214 Production engines. Any manufacturer obtaining certification under this part shall supply to the Administrator, upon...

40 CFR 94.214 - Production engines.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 40 Protection of Environment 21 2013-07-01 2013-07-01 false Production engines. 94.214 Section 94...) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Certification Provisions § 94.214 Production engines. Any manufacturer obtaining certification under this part shall supply to the Administrator, upon...

40 CFR 94.214 - Production engines.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 40 Protection of Environment 20 2011-07-01 2011-07-01 false Production engines. 94.214 Section 94...) CONTROL OF EMISSIONS FROM MARINE COMPRESSION-IGNITION ENGINES Certification Provisions § 94.214 Production engines. Any manufacturer obtaining certification under this part shall supply to the Administrator, upon...

48 CFR 214.407-3 - Other mistakes disclosed before award.

Code of Federal Regulations, 2013 CFR

2013-10-01

...) Missile Defense Agency: General Counsel, MDA. (ix) Defense Contract Management Agency: General Counsel... before award. 214.407-3 Section 214.407-3 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SEALED BIDDING Opening of...

48 CFR 214.407-3 - Other mistakes disclosed before award.

Code of Federal Regulations, 2012 CFR

2012-10-01

...) Missile Defense Agency: General Counsel, MDA. (ix) Defense Contract Management Agency: General Counsel... before award. 214.407-3 Section 214.407-3 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SEALED BIDDING Opening of...

48 CFR 214.407-3 - Other mistakes disclosed before award.

Code of Federal Regulations, 2011 CFR

2011-10-01

...) Missile Defense Agency: General Counsel, MDA. (ix) Defense Contract Management Agency: General Counsel... before award. 214.407-3 Section 214.407-3 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SEALED BIDDING Opening of...

48 CFR 214.407-3 - Other mistakes disclosed before award.

Code of Federal Regulations, 2014 CFR

2014-10-01

...) Missile Defense Agency: General Counsel, MDA. (ix) Defense Contract Management Agency: General Counsel... before award. 214.407-3 Section 214.407-3 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACTING METHODS AND CONTRACT TYPES SEALED BIDDING Opening of...

5 CFR 531.214 - Setting pay upon promotion.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Setting pay upon promotion. 531.214 Section 531.214 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY UNDER THE GENERAL SCHEDULE Determining Rate of Basic Pay Setting Pay When Appointment Or Position...

T-cell acute lymphoblastic leukemia associated with complex karyotype and SET-NUP214 rearrangement: a case study and review of the literature.

PubMed

Lee, Sang-Guk; Park, Tae Sung; Cho, Sun Young; Lim, Gayoung; Park, Gwang Jin; Oh, Seung Hwan; Cho, Eun Hae; Chong, So Young; Huh, Ji Young

2011-01-01

SET-NUP214 rearrangements have been rarely reported in T-cell acute lymphoblastic leukemia (T-ALL), acute undifferentiated leukemia, and acute myeloid leukemia, and most documented cases have been associated with normal karyotypes in conventional cytogenetic analyses. Here, we describe a novel case of T-ALL associated with a mediastinal mass and a SET-NUP214 rearrangement, which was masked by a complex karyotype at the time of initial diagnosis. Using multiplex reverse transcriptase-polymerase chain reaction analysis, we detected a cryptic SET-NUP214 rearrangement in our patient. As only 11 cases (including the present study) of T-ALL with SET-NUP214 rearrangement have been reported, the clinical features and treatment outcomes have not been fully determined. Further studies are necessary to evaluate the incidence of SET-NUP214 rearrangement in T-ALL patients and the treatment responses as well as prognosis of these patients.

49 CFR 214.303 - Railroad on-track safety programs, generally.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Railroad on-track safety programs, generally. 214... RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.303 Railroad on-track safety programs, generally. (a) Each railroad to which this part applies...

48 CFR 1233.214 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2010 CFR

2010-10-01

... resolution (ADR). 1233.214 Section 1233.214 Federal Acquisition Regulations System DEPARTMENT OF... Alternative dispute resolution (ADR). (c) The Administrative Dispute Resolution Act (ADRA) of 1990, Public Law... purposes. ADR procedures may be used when: (1) There is mutual consent by the parties to participate in the...

48 CFR 1233.214 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2011 CFR

2011-10-01

... resolution (ADR). 1233.214 Section 1233.214 Federal Acquisition Regulations System DEPARTMENT OF... Alternative dispute resolution (ADR). (c) The Administrative Dispute Resolution Act (ADRA) of 1990, Public Law... purposes. ADR procedures may be used when: (1) There is mutual consent by the parties to participate in the...

24 CFR 214.107 - Approval by HUD.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Approval by HUD. 214.107 Section 214.107 Housing and Urban Development Regulations Relating to Housing and Urban Development (Continued... DEVELOPMENT MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES HOUSING...

14 CFR 302.214 - Oral evidentiary hearing.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Oral evidentiary hearing. 302.214 Section 302.214 Aeronautics and Space OFFICE OF THE SECRETARY, DEPARTMENT OF TRANSPORTATION (AVIATION... administrative law judge. The issues will be those set forth in the order establishing further procedures. The...

24 CFR 214.313 - Housing counseling fees.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Housing counseling fees. 214.313... HOUSING COUNSELING PROGRAM Program Administration § 214.313 Housing counseling fees. (a) Participating agencies may charge reasonable and customary fees for housing education and counseling services, as long as...

24 CFR 214.100 - General.

Code of Federal Regulations, 2014 CFR

2014-04-01

... MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES HOUSING COUNSELING PROGRAM Approval and Disapproval of Housing Counseling Agencies § 214.100 General. An organization may be approved by HUD as a HUD-approved housing counseling agency upon meeting the requirements of § 214.103 and...

24 CFR 214.313 - Housing counseling fees.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Housing counseling fees. 214.313... HOUSING COUNSELING PROGRAM Program Administration § 214.313 Housing counseling fees. (a) Participating agencies may charge reasonable and customary fees for housing education and counseling services, as long as...

24 CFR 214.100 - General.

Code of Federal Regulations, 2011 CFR

2011-04-01

... MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES HOUSING COUNSELING PROGRAM Approval and Disapproval of Housing Counseling Agencies § 214.100 General. An organization may be approved by HUD as a HUD-approved housing counseling agency upon meeting the requirements of § 214.103 and...

24 CFR 214.100 - General.

Code of Federal Regulations, 2012 CFR

2012-04-01

... MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES HOUSING COUNSELING PROGRAM Approval and Disapproval of Housing Counseling Agencies § 214.100 General. An organization may be approved by HUD as a HUD-approved housing counseling agency upon meeting the requirements of § 214.103 and...

24 CFR 214.313 - Housing counseling fees.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Housing counseling fees. 214.313... HOUSING COUNSELING PROGRAM Program Administration § 214.313 Housing counseling fees. (a) Participating agencies may charge reasonable and customary fees for housing education and counseling services, as long as...

24 CFR 214.313 - Housing counseling fees.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 24 Housing and Urban Development 2 2013-04-01 2013-04-01 false Housing counseling fees. 214.313... HOUSING COUNSELING PROGRAM Program Administration § 214.313 Housing counseling fees. (a) Participating agencies may charge reasonable and customary fees for housing education and counseling services, as long as...

24 CFR 214.100 - General.

Code of Federal Regulations, 2013 CFR

2013-04-01

... MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES HOUSING COUNSELING PROGRAM Approval and Disapproval of Housing Counseling Agencies § 214.100 General. An organization may be approved by HUD as a HUD-approved housing counseling agency upon meeting the requirements of § 214.103 and...

24 CFR 214.313 - Housing counseling fees.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Housing counseling fees. 214.313... HOUSING COUNSELING PROGRAM Program Administration § 214.313 Housing counseling fees. (a) Participating agencies may charge reasonable and customary fees for housing education and counseling services, as long as...

24 CFR 214.100 - General.

Code of Federal Regulations, 2010 CFR

2010-04-01

... MORTGAGE AND LOAN INSURANCE PROGRAMS UNDER NATIONAL HOUSING ACT AND OTHER AUTHORITIES HOUSING COUNSELING PROGRAM Approval and Disapproval of Housing Counseling Agencies § 214.100 General. An organization may be approved by HUD as a HUD-approved housing counseling agency upon meeting the requirements of § 214.103 and...

48 CFR 52.214-16 - Minimum Bid Acceptance Period.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Minimum Bid Acceptance Period. 52.214-16 Section 52.214-16 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... date specified in this solicitation for receipt of bids. (b) This provision supersedes any language...

22 CFR 214.15 - Changes in membership.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Changes in membership. 214.15 Section 214.15 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE MANAGEMENT Establishment of... Management Officer and the Office of the General Counsel, and are approved by the A.I.D. Administrator. ...

27 CFR 21.4 - Related regulations.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Related regulations. 21.4 Section 21.4 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... regulations. The procedural and substantive requirements relative to the production of denatured alcohol and...

27 CFR 21.4 - Related regulations.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Related regulations. 21.4 Section 21.4 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT... regulations. The procedural and substantive requirements relative to the production of denatured alcohol and...

5 CFR 214.204 - Interchange agreements.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 5 Administrative Personnel 1 2013-01-01 2013-01-01 false Interchange agreements. 214.204 Section... EXECUTIVE SERVICE General Provisions § 214.204 Interchange agreements. (a) In accordance with 5 CFR 6.7, OPM... Service (SES) may, pursuant to legislative and regulatory authorities, enter into an agreement providing...

5 CFR 214.204 - Interchange agreements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 5 Administrative Personnel 1 2011-01-01 2011-01-01 false Interchange agreements. 214.204 Section... EXECUTIVE SERVICE General Provisions § 214.204 Interchange agreements. (a) In accordance with 5 CFR 6.7, OPM... Service (SES) may, pursuant to legislative and regulatory authorities, enter into an agreement providing...

48 CFR 52.214-16 - Minimum Bid Acceptance Period.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 2 2012-10-01 2012-10-01 false Minimum Bid Acceptance Period. 52.214-16 Section 52.214-16 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... date specified in this solicitation for receipt of bids. (b) This provision supersedes any language...

48 CFR 52.214-16 - Minimum Bid Acceptance Period.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 2 2013-10-01 2013-10-01 false Minimum Bid Acceptance Period. 52.214-16 Section 52.214-16 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... date specified in this solicitation for receipt of bids. (b) This provision supersedes any language...

48 CFR 52.214-16 - Minimum Bid Acceptance Period.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 2 2011-10-01 2011-10-01 false Minimum Bid Acceptance Period. 52.214-16 Section 52.214-16 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... date specified in this solicitation for receipt of bids. (b) This provision supersedes any language...

48 CFR 52.214-16 - Minimum Bid Acceptance Period.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 2 2014-10-01 2014-10-01 false Minimum Bid Acceptance Period. 52.214-16 Section 52.214-16 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... date specified in this solicitation for receipt of bids. (b) This provision supersedes any language...

9 CFR 93.214 - Import permit and declaration for poultry.

Code of Federal Regulations, 2010 CFR

2010-01-01

... poultry. 93.214 Section 93.214 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE EXPORTATION AND IMPORTATION OF ANIMALS (INCLUDING POULTRY) AND ANIMAL PRODUCTS IMPORTATION OF CERTAIN ANIMALS, BIRDS, FISH, AND POULTRY, AND CERTAIN ANIMAL, BIRD, AND POULTRY PRODUCTS...

24 CFR 214.307 - Performance review.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Performance review. 214.307 Section... COUNSELING PROGRAM Program Administration § 214.307 Performance review. (a) HUD may conduct periodic on-site or desk performance reviews of all participating agencies. (b) The performance review will consist of...

24 CFR 214.307 - Performance review.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Performance review. 214.307 Section... COUNSELING PROGRAM Program Administration § 214.307 Performance review. (a) HUD may conduct periodic on-site or desk performance reviews of all participating agencies. (b) The performance review will consist of...

24 CFR 214.307 - Performance review.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Performance review. 214.307 Section... COUNSELING PROGRAM Program Administration § 214.307 Performance review. (a) HUD may conduct periodic on-site or desk performance reviews of all participating agencies. (b) The performance review will consist of...

49 CFR 214.341 - Roadway maintenance machines.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Roadway maintenance machines. 214.341 Section 214... Roadway maintenance machines. (a) Each employer shall include in its on-track safety program specific provisions for the safety of roadway workers who operate or work near roadway maintenance machines. Those...

22 CFR 214.11 - Establishment and chartering requirements.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Establishment and chartering requirements. 214.11 Section 214.11 Foreign Relations AGENCY FOR INTERNATIONAL DEVELOPMENT ADVISORY COMMITTEE... certification that creation of the Committee is in the public interest and a description of the nature and...

31 CFR 31.214 - Limitations on concurrent activities.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 1 2010-07-01 2010-07-01 false Limitations on concurrent activities. 31.214 Section 31.214 Money and Finance: Treasury Office of the Secretary of the Treasury TROUBLED... assets, the retained entity, management officials performing work under the arrangement, and key...

34 CFR 682.214 - Compliance with equal credit opportunity requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false Compliance with equal credit opportunity requirements. 682.214 Section 682.214 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF POSTSECONDARY EDUCATION, DEPARTMENT OF EDUCATION FEDERAL FAMILY EDUCATION LOAN (FFEL) PROGRAM...

5 CFR 531.214 - Setting pay upon promotion.

Code of Federal Regulations, 2011 CFR

2011-01-01

... Section 531.214 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PAY... Changes § 531.214 Setting pay upon promotion. (a) General. An agency must set an employee's payable rate... rules in this section. (b) Geographic conversion. When an employee's official worksite is changed to a...

6 CFR 21.4 - Definitions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 6 Domestic Security 1 2013-01-01 2013-01-01 false Definitions. 21.4 Section 21.4 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY NONDISCRIMINATION ON THE BASIS OF RACE, COLOR, OR NATIONAL ORIGIN IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE FROM THE DEPARTMENT OF...

6 CFR 21.4 - Definitions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 6 Domestic Security 1 2014-01-01 2014-01-01 false Definitions. 21.4 Section 21.4 Domestic Security DEPARTMENT OF HOMELAND SECURITY, OFFICE OF THE SECRETARY NONDISCRIMINATION ON THE BASIS OF RACE, COLOR, OR NATIONAL ORIGIN IN PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE FROM THE DEPARTMENT OF...

34 CFR 685.214 - Closed school discharge.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 34 Education 3 2010-07-01 2010-07-01 false Closed school discharge. 685.214 Section 685.214... Closed school discharge. (a) General. (1) The Secretary discharges the borrower's (and any endorser's... made because the school at which the borrower (or student) was enrolled closed, as described in...

48 CFR 1433.214 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Alternative dispute resolution (ADR). 1433.214 Section 1433.214 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR... dispute resolution (ADR). DOI strongly encourages the use of ADR in the resolution of disputes in lieu of...

48 CFR 1433.214 - Alternative dispute resolution (ADR).

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Alternative dispute resolution (ADR). 1433.214 Section 1433.214 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR... dispute resolution (ADR). DOI strongly encourages the use of ADR in the resolution of disputes in lieu of...

49 CFR 214.347 - Training and qualification for lone workers.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Training and qualification for lone workers. 214... § 214.347 Training and qualification for lone workers. Each lone worker shall be trained and qualified... training and qualification for lone workers shall include, as a minimum, consideration of the following...

49 CFR 214.345 - Training for all roadway workers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Training for all roadway workers. 214.345 Section... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.345 Training for all roadway workers. The training of all roadway workers shall include, as a minimum, the...

49 CFR 214.347 - Training and qualification for lone workers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Training and qualification for lone workers. 214... § 214.347 Training and qualification for lone workers. Each lone worker shall be trained and qualified... training and qualification for lone workers shall include, as a minimum, consideration of the following...

49 CFR 214.345 - Training for all roadway workers.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Training for all roadway workers. 214.345 Section... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.345 Training for all roadway workers. The training of all roadway workers shall include, as a minimum, the...

48 CFR 633.214-70 - Alternative dispute resolution.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Alternative dispute resolution. 633.214-70 Section 633.214-70 Federal Acquisition Regulations System DEPARTMENT OF STATE GENERAL... situations; (vi) There is a need for independent expert analysis; or, (vii) The claim has merit but its value...

48 CFR 36.214 - Special procedures for price negotiation in construction contracting.

Code of Federal Regulations, 2013 CFR

2013-10-01

... price negotiation in construction contracting. 36.214 Section 36.214 Federal Acquisition Regulations... negotiation in construction contracting. (a) Agencies shall follow the policies and procedures in part 15 when... scope of the work. If negotiations reveal errors in the Government estimate, the estimate shall be...

48 CFR 36.214 - Special procedures for price negotiation in construction contracting.

Code of Federal Regulations, 2011 CFR

2011-10-01

... price negotiation in construction contracting. 36.214 Section 36.214 Federal Acquisition Regulations... negotiation in construction contracting. (a) Agencies shall follow the policies and procedures in part 15 when... scope of the work. If negotiations reveal errors in the Government estimate, the estimate shall be...

48 CFR 36.214 - Special procedures for price negotiation in construction contracting.

Code of Federal Regulations, 2012 CFR

2012-10-01

... price negotiation in construction contracting. 36.214 Section 36.214 Federal Acquisition Regulations... negotiation in construction contracting. (a) Agencies shall follow the policies and procedures in part 15 when... scope of the work. If negotiations reveal errors in the Government estimate, the estimate shall be...

48 CFR 36.214 - Special procedures for price negotiation in construction contracting.

Code of Federal Regulations, 2014 CFR

2014-10-01

... price negotiation in construction contracting. 36.214 Section 36.214 Federal Acquisition Regulations... negotiation in construction contracting. (a) Agencies shall follow the policies and procedures in part 15 when... scope of the work. If negotiations reveal errors in the Government estimate, the estimate shall be...

48 CFR 36.214 - Special procedures for price negotiation in construction contracting.

Code of Federal Regulations, 2010 CFR

2010-10-01

... price negotiation in construction contracting. 36.214 Section 36.214 Federal Acquisition Regulations... negotiation in construction contracting. (a) Agencies shall follow the policies and procedures in part 15 when... scope of the work. If negotiations reveal errors in the Government estimate, the estimate shall be...

48 CFR 52.214-34 - Submission of Offers in the English Language.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Submission of Offers in the English Language. 52.214-34 Section 52.214-34 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of...

48 CFR 1852.214-70 - Caution to offerors furnishing descriptive literature.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Caution to offerors furnishing descriptive literature. 1852.214-70 Section 1852.214-70 Federal Acquisition Regulations System... literature that includes language reserving to the bidder the right to deviate from the requirements of the...

29 CFR 825.214 - Employee right to reinstatement.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 29 Labor 3 2010-07-01 2010-07-01 false Employee right to reinstatement. 825.214 Section 825.214 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR OTHER LAWS THE FAMILY AND MEDICAL LEAVE ACT OF 1993 Employee Leave Entitlements Under the Family and Medical...

30 CFR 75.214 - Supplemental support materials, equipment and tools.

Code of Federal Regulations, 2010 CFR

2010-07-01

... tools. 75.214 Section 75.214 Mineral Resources MINE SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR COAL MINE SAFETY AND HEALTH MANDATORY SAFETY STANDARDS-UNDERGROUND COAL MINES Roof Support § 75... accessible location on each working section or within four crosscuts of each working section. (b) The...

23 CFR 973.214 - Indian lands congestion management system (CMS).

Code of Federal Regulations, 2014 CFR

2014-04-01

... 23 Highways 1 2014-04-01 2014-04-01 false Indian lands congestion management system (CMS). 973.214... HIGHWAYS MANAGEMENT SYSTEMS PERTAINING TO THE BUREAU OF INDIAN AFFAIRS AND THE INDIAN RESERVATION ROADS PROGRAM Bureau of Indian Affairs Management Systems § 973.214 Indian lands congestion management system...

36 CFR 2.14 - Sanitation and refuse.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 36 Parks, Forests, and Public Property 1 2012-07-01 2012-07-01 false Sanitation and refuse. 2.14 Section 2.14 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR... washing food, clothing, dishes, or other property at public water outlets, fixtures or pools, except at...

36 CFR 2.14 - Sanitation and refuse.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 36 Parks, Forests, and Public Property 1 2013-07-01 2013-07-01 false Sanitation and refuse. 2.14 Section 2.14 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR... washing food, clothing, dishes, or other property at public water outlets, fixtures or pools, except at...

36 CFR 2.14 - Sanitation and refuse.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 36 Parks, Forests, and Public Property 1 2014-07-01 2014-07-01 false Sanitation and refuse. 2.14 Section 2.14 Parks, Forests, and Public Property NATIONAL PARK SERVICE, DEPARTMENT OF THE INTERIOR... washing food, clothing, dishes, or other property at public water outlets, fixtures or pools, except at...

46 CFR 199.214 - Immersion suits and thermal protective aids.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 46 Shipping 7 2014-10-01 2014-10-01 false Immersion suits and thermal protective aids. 199.214... Passenger Vessels § 199.214 Immersion suits and thermal protective aids. (a) Each passenger vessel must... an immersion suit. (c) The immersion suits and thermal protective aids required under paragraphs (a...

46 CFR 199.214 - Immersion suits and thermal protective aids.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 46 Shipping 7 2011-10-01 2011-10-01 false Immersion suits and thermal protective aids. 199.214... Passenger Vessels § 199.214 Immersion suits and thermal protective aids. (a) Each passenger vessel must... an immersion suit. (c) The immersion suits and thermal protective aids required under paragraphs (a...

46 CFR 199.214 - Immersion suits and thermal protective aids.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 46 Shipping 7 2013-10-01 2013-10-01 false Immersion suits and thermal protective aids. 199.214... Passenger Vessels § 199.214 Immersion suits and thermal protective aids. (a) Each passenger vessel must... an immersion suit. (c) The immersion suits and thermal protective aids required under paragraphs (a...

46 CFR 199.214 - Immersion suits and thermal protective aids.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 46 Shipping 7 2012-10-01 2012-10-01 false Immersion suits and thermal protective aids. 199.214... Passenger Vessels § 199.214 Immersion suits and thermal protective aids. (a) Each passenger vessel must... an immersion suit. (c) The immersion suits and thermal protective aids required under paragraphs (a...

40 CFR 761.214 - Retention of manifest records.

Code of Federal Regulations, 2013 CFR

2013-07-01

... PROHIBITIONS PCB Waste Disposal Records and Reports § 761.214 Retention of manifest records. (a)(1) A generator... Section 761.214 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) TOXIC SUBSTANCES... receives a signed copy from the designated facility which received the PCB waste. This signed copy must be...

40 CFR 761.214 - Retention of manifest records.

Code of Federal Regulations, 2014 CFR

2014-07-01

... PROHIBITIONS PCB Waste Disposal Records and Reports § 761.214 Retention of manifest records. (a)(1) A generator... Section 761.214 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) TOXIC SUBSTANCES... receives a signed copy from the designated facility which received the PCB waste. This signed copy must be...

48 CFR 52.214-34 - Submission of Offers in the English Language.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 2 2013-10-01 2013-10-01 false Submission of Offers in the English Language. 52.214-34 Section 52.214-34 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of...

48 CFR 52.214-34 - Submission of Offers in the English Language.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 2 2014-10-01 2014-10-01 false Submission of Offers in the English Language. 52.214-34 Section 52.214-34 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of...

48 CFR 52.214-34 - Submission of Offers in the English Language.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 2 2012-10-01 2012-10-01 false Submission of Offers in the English Language. 52.214-34 Section 52.214-34 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of...

18 CFR 281.214 - Notice, complaint and remedy.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Notice, complaint and remedy. 281.214 Section 281.214 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY... determines that a violation of this subpart has occurred, it shall take whatever action it deems appropriate...

20 CFR 645.214 - How will Welfare-to-Work participant eligibility be determined?

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 3 2010-04-01 2010-04-01 false How will Welfare-to-Work participant eligibility be determined? 645.214 Section 645.214 Employees' Benefits EMPLOYMENT AND TRAINING ADMINISTRATION, DEPARTMENT OF LABOR PROVISIONS GOVERNING WELFARE-TO-WORK GRANTS General Program and Administrative...

49 CFR 214.103 - Fall protection, generally.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Fall protection, generally. 214.103 Section 214... Fall protection, generally. (a) Except as provided in paragraphs (b) through (d) of this section, when... shall use a personal fall arrest system or safety net system. All fall protection systems required by...

49 CFR 214.103 - Fall protection, generally.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Fall protection, generally. 214.103 Section 214... Fall protection, generally. (a) Except as provided in paragraphs (b) through (d) of this section, when... shall use a personal fall arrest system or safety net system. All fall protection systems required by...

49 CFR 214.103 - Fall protection, generally.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Fall protection, generally. 214.103 Section 214... Fall protection, generally. (a) Except as provided in paragraphs (b) through (d) of this section, when... shall use a personal fall arrest system or safety net system. All fall protection systems required by...

49 CFR 214.103 - Fall protection, generally.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Fall protection, generally. 214.103 Section 214... Fall protection, generally. (a) Except as provided in paragraphs (b) through (d) of this section, when... shall use a personal fall arrest system or safety net system. All fall protection systems required by...

49 CFR 214.103 - Fall protection, generally.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Fall protection, generally. 214.103 Section 214... Fall protection, generally. (a) Except as provided in paragraphs (b) through (d) of this section, when... shall use a personal fall arrest system or safety net system. All fall protection systems required by...

49 CFR 214.339 - Audible warning from trains.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Audible warning from trains. 214.339 Section 214... Audible warning from trains. Each railroad shall require that the locomotive whistle be sounded, and the locomotive bell be rung, by trains approaching roadway workers on or about the track. Such audible warning...

49 CFR 214.339 - Audible warning from trains.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false Audible warning from trains. 214.339 Section 214... Audible warning from trains. Each railroad shall require that the locomotive whistle be sounded, and the locomotive bell be rung, by trains approaching roadway workers on or about the track. Such audible warning...

49 CFR 214.339 - Audible warning from trains.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Audible warning from trains. 214.339 Section 214... Audible warning from trains. Each railroad shall require that the locomotive whistle be sounded, and the locomotive bell be rung, by trains approaching roadway workers on or about the track. Such audible warning...

49 CFR 214.339 - Audible warning from trains.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false Audible warning from trains. 214.339 Section 214... Audible warning from trains. Each railroad shall require that the locomotive whistle be sounded, and the locomotive bell be rung, by trains approaching roadway workers on or about the track. Such audible warning...

49 CFR 214.339 - Audible warning from trains.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Audible warning from trains. 214.339 Section 214... Audible warning from trains. Each railroad shall require that the locomotive whistle be sounded, and the locomotive bell be rung, by trains approaching roadway workers on or about the track. Such audible warning...

7 CFR 58.214 - General construction, repair and installation.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 3 2010-01-01 2010-01-01 false General construction, repair and installation. 58.214... Service 1 Equipment and Utensils § 58.214 General construction, repair and installation. All equipment and utensils necessary to the manufacture of dry milk products, including pasteurizer, timing-pump or device...

36 CFR 214.19 - Procedures for discretionary review.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 36 Parks, Forests, and Public Property 2 2014-07-01 2014-07-01 false Procedures for discretionary review. 214.19 Section 214.19 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... time to assist in making that determination. If a request for the record is made, it must be...

25 CFR 214.13 - Diligence; annual expenditures; mining records.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Diligence; annual expenditures; mining records. 214.13... OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.13 Diligence; annual expenditures; mining records. (a) Lessees shall exercise diligence in the conduct of prospecting and mining...

46 CFR 199.214 - Immersion suits and thermal protective aids.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 46 Shipping 7 2010-10-01 2010-10-01 false Immersion suits and thermal protective aids. 199.214... Passenger Vessels § 199.214 Immersion suits and thermal protective aids. (a) Each passenger vessel must... an immersion suit. (c) The immersion suits and thermal protective aids required under paragraphs (a...

48 CFR 2052.214-73 - Timely receipt of bids.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Timely receipt of bids. 2052.214-73 Section 2052.214-73 Federal Acquisition Regulations System NUCLEAR REGULATORY COMMISSION... is a secure facility with perimeter access-control and NRC personnel are only available to receive...

48 CFR 2052.214-73 - Timely receipt of bids.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false Timely receipt of bids. 2052.214-73 Section 2052.214-73 Federal Acquisition Regulations System NUCLEAR REGULATORY COMMISSION... is a secure facility with perimeter access-control and NRC personnel are only available to receive...

48 CFR 2052.214-73 - Timely receipt of bids.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false Timely receipt of bids. 2052.214-73 Section 2052.214-73 Federal Acquisition Regulations System NUCLEAR REGULATORY COMMISSION... is a secure facility with perimeter access-control and NRC personnel are only available to receive...

48 CFR 2052.214-73 - Timely receipt of bids.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false Timely receipt of bids. 2052.214-73 Section 2052.214-73 Federal Acquisition Regulations System NUCLEAR REGULATORY COMMISSION... is a secure facility with perimeter access-control and NRC personnel are only available to receive...

47 CFR 63.21 - Conditions applicable to all international Section 214 authorizations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 3 2010-10-01 2010-10-01 false Conditions applicable to all international... STATUS Extensions and Supplements § 63.21 Conditions applicable to all international Section 214 authorizations. International carriers authorized under Section 214 of the Communications Act of 1934, as amended...

47 CFR 63.12 - Processing of international Section 214 applications.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 47 Telecommunication 3 2010-10-01 2010-10-01 false Processing of international Section 214... and Supplements § 63.12 Processing of international Section 214 applications. (a) Except as provided... part shall be granted by the Commission 14 days after the date of public notice listing the application...

42 CFR 422.214 - Special rules for services furnished by noncontract providers.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 42 Public Health 3 2010-10-01 2010-10-01 false Special rules for services furnished by noncontract providers. 422.214 Section 422.214 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICARE PROGRAM MEDICARE ADVANTAGE PROGRAM Relationships With...

12 CFR 21.4 - Report.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 12 Banks and Banking 1 2014-01-01 2014-01-01 false Report. 21.4 Section 21.4 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY MINIMUM SECURITY DEVICES AND PROCEDURES, REPORTS OF... Report. The security officer for a national bank shall report at least annually to the bank's board of...

12 CFR 21.4 - Report.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 12 Banks and Banking 1 2012-01-01 2012-01-01 false Report. 21.4 Section 21.4 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY MINIMUM SECURITY DEVICES AND PROCEDURES, REPORTS OF... Report. The security officer for a national bank shall report at least annually to the bank's board of...

12 CFR 21.4 - Report.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Report. 21.4 Section 21.4 Banks and Banking COMPTROLLER OF THE CURRENCY, DEPARTMENT OF THE TREASURY MINIMUM SECURITY DEVICES AND PROCEDURES, REPORTS OF... Report. The security officer for a national bank shall report at least annually to the bank's board of...

49 CFR 214.331 - Definite train location.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Definite train location. 214.331 Section 214.331... location. A roadway worker may establish on-track safety by using definite train location only where... may only use definite train location to establish on-track safety at points where such procedures were...

AP214, an analogue of α-melanocyte-stimulating hormone, ameliorates sepsis-induced acute kidney injury and mortality

PubMed Central

Doi, Kent; Hu, Xuzhen; Yuen, Peter S.T.; Leelahavanichkul, Asada; Yasuda, Hideo; Kim, Soo Mi; Schnermann, Jürgen; Jonassen, Thomas E.N.; Frøkiær, Jørgen; Nielsen, Søren; Star, Robert A.

2008-01-01

Sepsis remains a serious problem in critically ill patients with the mortality increasing to over half when there is attendant acute kidney injury. α-Melanocyte-stimulating hormone is a potent anti-inflammatory cytokine that inhibits many forms of inflammation including that with acute kidney injury. We tested whether a new α-melanocyte-stimulating hormone analogue (AP214), which has increased binding affinity to melanocortin receptors, improves sepsis-induced kidney injury and mortality using a cecal ligation and puncture mouse model. In the lethal cecal ligation-puncture model of sepsis, severe hypotension and bradycardia resulted and AP214 attenuated acute kidney injury of the lethal model with a bell-shaped dose-response curve. An optimum AP214 dose reduced acute kidney injury even when it was administered 6 hr after surgery and it significantly improved blood pressure and heart rate. AP214 reduced serum TNF-α and IL-10 levels with a bell-shaped dose-response curve. Additionally; NF-κB activation in the kidney and spleen, and splenocyte apoptosis were decreased by the treatment. AP214 significantly improved survival in both lethal and sublethal models. We have shown that AP214 improves hemodynamic failure, acute kidney injury, mortality and splenocyte apoptosis attenuating pro- and anti-inflammatory actions due to sepsis. PMID:18354376

Antiviral function of tilapia hepcidin 1-5 and its modulation of immune-related gene expressions against infectious pancreatic necrosis virus (IPNV) in Chinook salmon embryo (CHSE)-214 cells.

PubMed

Rajanbabu, Venugopal; Chen, Jyh-Yih

2011-01-01

Antimicrobial peptides, small cysteine-rich molecules, play vital roles in host defense mechanisms against pathogen infection. Recently, tilapia hepcidin (TH)1-5, was characterized, and its antimicrobial functions against several pathogens were reported. Herein, we investigated the antiviral functions of TH1-5 against infectious pancreatic necrosis virus (IPNV) in Chinook salmon embryo (CHSE)-214 cells. The presence of TH1-5 enhanced the survival of CHSE-214 cells infected with IPNV. Additionally, the number of plaques formed by the cytopathic effect of IPNV in CHSE-214 cells decreased when IPNV was preincubated with TH1-5. This observation demonstrates the antiviral function of TH1-5. Real-time PCR studies showed the modulation of interleukin, annexin, and other viral-responsive gene expressions by TH1-5. When TH1-5 and IPNV were used to co-treat CHSE-214 cells, then cells were re-challenged with IPNV at 24h, the cells did not survive the IPNV infection. This shows that in the absence of TH1-5, viral re-challenge killed CHSE-214 cells. In conclusion TH1-5 protected CHSE-214 cells against IPNV by direct antimicrobial and immunomodulatory functions. Copyright © 2010 Elsevier Ltd. All rights reserved.

Determining the charged fractions of 218Po and 214Pb using an environmental gamma-ray and Rn detector.

PubMed

Maiello, M L; Harley, N H

1989-07-01

The rate of 218Po and 214Pb atoms collected electrostatically inside an environmental gamma-ray and 222Rn detector (EGARD) was measured. These measurements were used to directly infer the charged fraction of 218Po and to calculate the charged fraction of 214Pb. Thirty-two percent of the 218Po was collected electrostatically using approximately -1500 V on a 2.54 cm diameter Mylar covered disc inside a vented A1 EGARD of 1 L volume. About 91% of the 214Pb is collected electrostatically under the same conditions. The measurements were performed in a calibrated 222Rn test chamber at the Environmental Measurements Laboratory (EML) using the Thomas alpha-counting method with 222Rn concentrations averaging about 4300 Bq m-3. The atomic collection rates were used with other measured quantities to calculate the thermoluminescent dosimeter (TLD) signal acquired from EGARD for exposure to 1 Bq m-3 of 222Rn. The calculations account for 222Rn progeny collection using a Teflon electret and alpha and beta detection using TLDs inside EGARD. The measured quantities include the energies of 218Po and 214Po alpha-particles degraded by passage through the 25 microns thick electret. The TLD responses to these alpha- and beta-particles with an average energy approaching that obtained from the combined spectra of 214Pb and 214Bi were also measured. The calculated calibration factor is within 30% of the value obtained by exposing EGARD to a known concentration of 222Rn. This result supports our charged fraction estimates for 218Po and 214Pb.

Costimulation dependent expression of miR-214 increases the ability of T cells to proliferate by targeting Pten

PubMed Central

Jindra, Peter T.; Bagley, Jessamyn; Godwin, Jonathan G.; Iacomini, John

2010-01-01

T cell activation requires signaling through the T cell receptor (TCR) and costimulatory molecules such as CD28. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression post transcriptionally and are also known to be involved in lymphocyte development and function. Here we set out to examine potential roles of miRNAs in T cell activation by using genome-wide expression profiling to identify miRNAs differentially regulated following T cell activation. One of the miRNAs up-regulated after T cell activation, miR-214, was predicted to be capable of targeting Pten based on bioinformatics and reports suggesting that it targets Pten in ovarian tumor cells. Up-regulation of miR-214 in T cells inversely correlated with PTEN levels. In vivo, transcripts containing the 3' untranslated region (3' UTR) of Pten including the miR-214 target sequence were negatively regulated after T cell activation, and forced expression of miR-214 in T cells led to increased proliferation after stimulation. Blocking CD28 signaling in vivo prevented miR-214 up-regulation in alloreactive T cells. Stimulation of T cells through the TCR alone was not sufficient to result in upregulation of miR-214. Thus, costimulation dependent up-regulation of miR-214 promotes T cell activation by targeting the negative regulator Pten. Thus, the requirement for T cell costimulation is in part related to its ability to regulate expression of miRNAs that control T cell activation. PMID:20548023

A bone-resorption surface-targeting nanoparticle to deliver anti-miR214 for osteoporosis therapy

PubMed Central

Zhang, Shufan; Liu, Jiafan; Sun, Yao; Wang, Xiaogang

2017-01-01

With increasing fracture risks due to fragility, osteoporosis is a global health problem threatening postmenopausal women. In these patients, osteoclasts play leading roles in bone loss and fracture. How to inhibit osteoclast activity is the key issue for osteoporosis treatment. In recent years, miRNA-based gene therapy through gene regulation has been considered a potential therapeutic method. However, in light of the side effects, the use of therapeutic miRNAs in osteoporosis treatment is still limited by the lack of tissue/cell-specific delivery systems. Here, we developed polyurethane (PU) nanomicelles modified by the acidic peptide Asp8. Our data showed that without overt toxicity or eliciting an immune response, this delivery system encapsulated and selectively deliver miRNAs to OSCAR+ osteoclasts at bone-resorption surface in vivo. With the Asp8-PU delivery system, anti-miR214 was delivered to osteoclasts, and bone microarchitecture and bone mass were improved in ovariectomized osteoporosis mice. Therefore, Asp8-PU could be a useful bone-resorption surface-targeting delivery system for treatment of osteoclast-induced bone diseases and aging-related osteoporosis. PMID:29075114

Clinical Relevance of Pathogens Detected by Multiplex PCR in Blood of Very-Low-Birth Weight Infants with Suspected Sepsis - Multicentre Study of the German Neonatal Network.

PubMed

Tröger, Birte; Härtel, Christoph; Buer, Jan; Dördelmann, Michael; Felderhoff-Müser, Ursula; Höhn, Thomas; Hepping, Nico; Hillebrand, Georg; Kribs, Angela; Marissen, Janina; Olbertz, Dirk; Rath, Peter-Michael; Schmidtke, Susanne; Siegel, Jens; Herting, Egbert; Göpel, Wolfgang; Steinmann, Joerg; Stein, Anja

2016-01-01

In the German Neonatal Network (GNN) 10% of very-low-birth weight infants (VLBWI) suffer from blood-culture confirmed sepsis, while 30% of VLBWI develop clinical sepsis. Diagnosis of sepsis is a difficult task leading to potential over-treatment with antibiotics. This study aims to investigate whether the results of blood multiplex-PCR (SeptiFast®) for common sepsis pathogens are relevant for clinical decision making when sepsis is suspected in VLBWI. We performed a prospective, multi-centre study within the GNN including 133 VLBWI with 214 episodes of suspected late onset sepsis (LOS). In patients with suspected sepsis a multiplex-PCR (LightCycler SeptiFast MGRADE-test®) was performed from 100 μl EDTA blood in addition to center-specific laboratory biomarkers. The attending neonatologist documented whether the PCR-result, which was available after 24 to 48 hrs, had an impact on the choice of antibiotic drugs and duration of therapy. PCR was positive in 110/214 episodes (51%) and blood culture (BC) was positive in 55 episodes (26%). Both methods yielded predominantly coagulase-negative staphylococci (CoNS) followed by Escherichia coli and Staphylococcus aureus. In 214 BC-PCR paired samples concordant results were documented in 126 episodes (59%; n = 32 were concordant pathogen positive results, n = 94 were negative in both methods). In 65 episodes (30%) we found positive PCR results but negative BCs, with CoNS being identified in 43 (66%) of these samples. Multiplex-PCR results influenced clinical decision making in 30% of episodes, specifically in 18% for the choice of antimicrobial therapy and in 22% for the duration of antimicrobial therapy. Multiplex-PCR results had a moderate impact on clinical management in about one third of LOS-episodes. The main advantage of multiplex-PCR was the rapid detection of pathogens from micro-volume blood samples. In VLBWI limitations include risk of contamination, lack of resistance testing and high costs. The high rate of positive PCR results in episodes of negative BC might lead to overtreatment of infants which is associated with risk of mortality, antibiotic resistance, fungal sepsis and NEC.

29 CFR 778.214 - Benefit plans; including profit-sharing plans or trusts providing similar benefits.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 29 Labor 3 2011-07-01 2011-07-01 false Benefit plans; including profit-sharing plans or trusts providing similar benefits. 778.214 Section 778.214 Labor Regulations Relating to Labor (Continued) WAGE AND HOUR DIVISION, DEPARTMENT OF LABOR STATEMENTS OF GENERAL POLICY OR INTERPRETATION NOT DIRECTLY RELATED...

31 CFR 800.214 - Foreign government-controlled transaction.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance:Treasury 3 2011-07-01 2011-07-01 false Foreign government-controlled transaction. 800.214 Section 800.214 Money and Finance: Treasury Regulations Relating to Money and Finance... control of a U.S. business by a foreign government or a person controlled by or acting on behalf of a...

25 CFR 214.30 - Lessees must appoint local representative.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Lessees must appoint local representative. 214.30 Section... OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.30 Lessees must appoint local... assignee shall appoint a local or resident representative within the State, on whom the superintendent or...

25 CFR 214.30 - Lessees must appoint local representative.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 25 Indians 1 2011-04-01 2011-04-01 false Lessees must appoint local representative. 214.30 Section... OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.30 Lessees must appoint local... assignee shall appoint a local or resident representative within the State, on whom the superintendent or...

30 CFR 204.214 - Is minimum royalty due on a property for which I took relief?

Code of Federal Regulations, 2010 CFR

2010-07-01

... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Is minimum royalty due on a property for which I took relief? 204.214 Section 204.214 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT ALTERNATIVES FOR MARGINAL PROPERTIES Accounting and Auditing...

49 CFR 214.355 - Training and qualification in on-track safety for operators of roadway maintenance machines.

Code of Federal Regulations, 2011 CFR

2011-10-01

... operators of roadway maintenance machines. 214.355 Section 214.355 Transportation Other Regulations Relating... operators of roadway maintenance machines. (a) The training and qualification of roadway workers who operate roadway maintenance machines shall include, as a minimum: (1) Procedures to prevent a person from being...

49 CFR 214.355 - Training and qualification in on-track safety for operators of roadway maintenance machines.

Code of Federal Regulations, 2010 CFR

2010-10-01

... operators of roadway maintenance machines. 214.355 Section 214.355 Transportation Other Regulations Relating... operators of roadway maintenance machines. (a) The training and qualification of roadway workers who operate roadway maintenance machines shall include, as a minimum: (1) Procedures to prevent a person from being...

47 CFR 68.214 - Changes in other than “fully protected” premises wiring that serves fewer than four subscriber...

Code of Federal Regulations, 2010 CFR

2010-10-01

... premises wiring that serves fewer than four subscriber access lines. 68.214 Section 68.214... other than “fully protected” premises wiring that serves fewer than four subscriber access lines. Operations associated with the installation, connection, reconfiguration and removal (other than final...

48 CFR 2052.214-72 - Bid evaluation.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Bid evaluation. 2052.214-72... SOLICITATION PROVISIONS AND CONTRACT CLAUSES Text of Provisions and Clauses 2052.214-72 Bid evaluation. As... invitations for bids (paragraph “(f)” of this provision is optional): Bid Evaluation (JAN 1993) (a) Award will...

49 CFR 214.521 - Flagging equipment for on-track roadway maintenance machines and hi-rail vehicles.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Flagging equipment for on-track roadway maintenance machines and hi-rail vehicles. 214.521 Section 214.521 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY On-Track Roadway Maintenance...

49 CFR 214.525 - Towing with on-track roadway maintenance machines or hi-rail vehicles.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Towing with on-track roadway maintenance machines or hi-rail vehicles. 214.525 Section 214.525 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY On-Track Roadway Maintenance Machines and...

49 CFR 214.525 - Towing with on-track roadway maintenance machines or hi-rail vehicles.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 4 2011-10-01 2011-10-01 false Towing with on-track roadway maintenance machines or hi-rail vehicles. 214.525 Section 214.525 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY On-Track Roadway Maintenance Machines and...

49 CFR 214.521 - Flagging equipment for on-track roadway maintenance machines and hi-rail vehicles.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 4 2012-10-01 2012-10-01 false Flagging equipment for on-track roadway maintenance machines and hi-rail vehicles. 214.521 Section 214.521 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY On-Track Roadway Maintenance...

25 CFR 214.21 - Inspection of lessees' books and records.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 1 2010-04-01 2010-04-01 false Inspection of lessees' books and records. 214.21 Section... OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.21 Inspection of lessees' books... premises for the purpose of inspection, and their books and records showing manner of operations and...

25 CFR 214.21 - Inspection of lessees' books and records.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 25 Indians 1 2014-04-01 2014-04-01 false Inspection of lessees' books and records. 214.21 Section... OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.21 Inspection of lessees' books... premises for the purpose of inspection, and their books and records showing manner of operations and...

25 CFR 214.21 - Inspection of lessees' books and records.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 25 Indians 1 2012-04-01 2011-04-01 true Inspection of lessees' books and records. 214.21 Section... OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.21 Inspection of lessees' books... premises for the purpose of inspection, and their books and records showing manner of operations and...

25 CFR 214.21 - Inspection of lessees' books and records.

Code of Federal Regulations, 2013 CFR

2013-04-01

... 25 Indians 1 2013-04-01 2013-04-01 false Inspection of lessees' books and records. 214.21 Section... OSAGE RESERVATION LANDS, OKLAHOMA, FOR MINING, EXCEPT OIL AND GAS § 214.21 Inspection of lessees' books... premises for the purpose of inspection, and their books and records showing manner of operations and...

MicroRNA-214 controls skin and hair follicle development by modulating the activity of the Wnt pathway

PubMed Central

Ahmed, Mohammed I.; Alam, Majid; Emelianov, Vladimir U.; Poterlowicz, Krzysztof; Patel, Ankit; Sharov, Andrey A.; Mardaryev, Andrei N.

2014-01-01

Skin development is governed by complex programs of gene activation and silencing, including microRNA-dependent modulation of gene expression. Here, we show that miR-214 regulates skin morphogenesis and hair follicle (HF) cycling by targeting β-catenin, a key component of the Wnt signaling pathway. miR-214 exhibits differential expression patterns in the skin epithelium, and its inducible overexpression in keratinocytes inhibited proliferation, which resulted in formation of fewer HFs with decreased hair bulb size and thinner hair production. The inhibitory effects of miR-214 on HF development and cycling were associated with altered activities of multiple signaling pathways, including decreased expression of key Wnt signaling mediators β-catenin and Lef-1, and were rescued by treatment with pharmacological Wnt activators. Finally, we identify β-catenin as one of the conserved miR-214 targets in keratinocytes. These data provide an important foundation for further analyses of miR-214 as a key regulator of Wnt pathway activity and stem cell functions during normal tissue homeostasis, regeneration, and aging. PMID:25422376

Half-lives of 214Pb and 214Bi.

PubMed

Martz, D E; Langner, G H; Johnson, P R

1991-10-01

New measurements on chemically separated samples of 214Bi have yielded a mean half-life value of 19.71 +/- 0.02 min, where the error quoted is twice the standard deviation of the mean based on 23 decay runs. This result provides strong support for the historic 19.72 +/- 0.04 min half-life value and essentially excludes the 19.9-min value, both reported in previous studies. New measurements of the decay rate of 222Rn progeny activity initially in radioactive equilibrium have yielded a value of 26.89 +/- 0.03 min for the half-life of 214Pb, where the error quoted is twice the standard deviation of the mean based on 12 decay runs. This value is 0.1 min longer than the currently accepted 214Pb half-value of 26.8 min.

Effect of flow rate and lead/copper pipe sequence on lead release from service lines.

PubMed

Cartier, Clément; Arnold, Roger B; Triantafyllidou, Simoni; Prévost, Michèle; Edwards, Marc

2012-09-01

A pilot experiment examined lead leaching from four representative configurations of service lines including: (1) 100% lead (Pb), (2) 100% copper (Cu), (3) 50% Pb upstream of 50% Cu, and (4) 50% Pb-downstream of 50% Cu using a range of flow rates. The cumulative mass of lead release indicated that a typical partial replacement configuration (50% lead downstream of copper) did not provide a net reduction in lead when compared to 100% lead pipe (85 mg for 50% Pb-downstream versus 83 mg for 100%-Pb) due to galvanic and deposition corrosion. The partially replaced service line configuration also had a much greater likelihood of producing water with "spikes" of lead particulates at higher flow rates, while tending to produce lower levels of lead at very low flow rates. After the first 214 days the galvanic current between copper and lead was only reduced by 34%, proving that galvanic impacts can be highly persistent even in water with optimized corrosion control by dosing of zinc orthophosphate. Finally, this experiment raises concern about the low flow rates used during some prior home sampling events, which may underestimate exposure to lead during normal water use, especially when galvanic Pb:Cu connections are present. Copyright © 2012 Elsevier Ltd. All rights reserved.

49 CFR 214.529 - In-service failure of primary braking system.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 4 2014-10-01 2014-10-01 false In-service failure of primary braking system. 214... Maintenance Machines and Hi-Rail Vehicles § 214.529 In-service failure of primary braking system. (a) In the event of a total in-service failure of its primary braking system, an on-track roadway maintenance...

49 CFR 214.529 - In-service failure of primary braking system.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 4 2013-10-01 2013-10-01 false In-service failure of primary braking system. 214... Maintenance Machines and Hi-Rail Vehicles § 214.529 In-service failure of primary braking system. (a) In the event of a total in-service failure of its primary braking system, an on-track roadway maintenance...

12 CFR 5.24 - Conversion.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 1 2010-01-01 2010-01-01 false Conversion. 5.24 Section 5.24 Banks and Banking... ACTIVITIES Initial Activities § 5.24 Conversion. (a) Authority. 12 U.S.C. 35, 93a, 214a, 214b, 214c, and 2903... savings association. (d) Conversion of a state bank or Federal savings association to a national bank—(1...

12 CFR 5.24 - Conversion.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 12 Banks and Banking 1 2011-01-01 2011-01-01 false Conversion. 5.24 Section 5.24 Banks and Banking... ACTIVITIES Initial Activities § 5.24 Conversion. (a) Authority. 12 U.S.C. 35, 93a, 214a, 214b, 214c, and 2903... savings association. (d) Conversion of a state bank or Federal savings association to a national bank—(1...

49 CFR 214.517 - Retrofitting of existing on-track roadway maintenance machines manufactured on or after January 1...

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 4 2010-10-01 2010-10-01 false Retrofitting of existing on-track roadway maintenance machines manufactured on or after January 1, 1991. 214.517 Section 214.517 Transportation Other... Retrofitting of existing on-track roadway maintenance machines manufactured on or after January 1, 1991. In...

49 CFR 214.529 - In-service failure of primary braking system.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Maintenance Machines and Hi-Rail Vehicles § 214.529 In-service failure of primary braking system. (a) In the event of a total in-service failure of its primary braking system, an on-track roadway maintenance... 49 Transportation 4 2010-10-01 2010-10-01 false In-service failure of primary braking system. 214...

49 CFR 214.525 - Towing with on-track roadway maintenance machines or hi-rail vehicles.

Code of Federal Regulations, 2010 CFR

2010-10-01

... towing would cause the machine or hi-rail vehicle to exceed the capabilities of its braking system. In determining the limit of the braking system, the employer must consider the track grade (slope), as well as... or hi-rail vehicles. 214.525 Section 214.525 Transportation Other Regulations Relating to...

20 CFR 702.214 - Notice; form and content.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 20 Employees' Benefits 3 2011-04-01 2011-04-01 false Notice; form and content. 702.214 Section 702... Procedures Notice § 702.214 Notice; form and content. Notice shall be in writing on a form prescribed by the Director for this purpose; such form shall be made available to the employee or beneficiary by the employer...

Aluminium oxide nanoparticles induced morphological changes, cytotoxicity and oxidative stress in Chinook salmon (CHSE-214) cells.

PubMed

Srikanth, Koigoora; Mahajan, Amit; Pereira, Eduarda; Duarte, Armando Costa; Venkateswara Rao, Janapala

2015-10-01

Aluminium oxide nanoparticles (Al2 O3 NPs) are increasingly used in diverse applications that has raised concern about their safety. Recent studies suggested that Al2 O3 NPs induced oxidative stress may be the cause of toxicity in algae, Ceriodaphnia dubia, Caenorhabditis elegans and Danio rerio. However, there is paucity on the toxicity of Al2 O3 NPs on fish cell lines. The current study was aimed to investigate Al2 O3 NPs induced cytotoxicity, oxidative stress and morphological abnormality of Chinnok salmon cells (CHSE-214). A dose-dependent decline in cell viability was observed in CHSE-214 cells exposed to Al2 O3 NPs. Oxidative stress induced by Al2 O3 NPs in CHSE-214 cells has resulted in the significant reduction of superoxide dismutase, catalase and glutathione in a dose-dependent manner. However, a significant increase in glutathione sulfo-transferase and lipid peroxidation was observed in CHSE-214 cells exposed to Al2 O3 NPs in a dose-dependent manner. Significant morphological changes in CHSE-214 cells were observed when exposed to Al2 O3 NPs at 6, 12 and 24 h. The cells started to detach and appear spherical at 6 h followed by loss of cellular contents resulting in the shrinking of the cells. At 24 h, the cells started to disintegrate and resulted in cell death. Our data demonstrate that Al2 O3 NPs induce cytotoxicity and oxidative stress in a dose-dependent manner in CHSE-214 cells. Thus, our current work may serve as a base-line study for future evaluation of toxicity studies using CHSE-214 cells. Copyright © 2015 John Wiley & Sons, Ltd.

Cytochrome b6 arginine 214 of Synechococcus sp. PCC 7002, a key residue for quinone-reductase site function and turnover of the cytochrome bf complex.

PubMed

Nelson, Matthew E; Finazzi, Giovanni; Wang, Qing Jun; Middleton-Zarka, Kelly A; Whitmarsh, John; Kallas, Toivo

2005-03-18

Quinone-reductase (Q(i)) domains of cyanobacterial/chloroplast cytochrome bf and bacterial/mitochondrial bc complexes differ markedly, and the cytochrome bf Q(i) site mechanism remains largely enigmatic. To investigate the bf Q(i) domain, we constructed the mutation R214H, which substitutes histidine for a conserved arginine in the cytochrome b(6) polypeptide of the cyanobacterium Synechococcus sp. SPCC 7002. At high light intensity, the R214H mutant grew approximately 2.5-fold more slowly than the wild type. Slower growth arose from correspondingly slower overall turnover of the bf complex. Specifically, as shown in single flash turnover experiments of cytochrome b(6) reduction and oxidation, the R214H mutation partially blocked electron transfer to the Q(i) site, mimicking the effect of the Q(i) site inhibitor 2-N-4-hydroxyquinoline-N-oxide. The kinetics of cytochrome b(6) oxidation were largely unaffected by hydrogen-deuterium exchange in the mutant but were slowed considerably in the wild type. This suggests that although protonation events influenced the kinetics of cytochrome b(6) oxidation at the Q(i) site in the wild type, electron flow limited this reaction in the R214H mutant. Redox titration of membranes revealed midpoint potentials (E(m,7)) of the two b hemes similar to those in the wild type. Our data define cytochrome b(6) Arg(214) as a key residue for Q(i) site catalysis and turnover of the cytochrome bf complex. In the recent cytochrome bf structures, Arg(214) lies near the Q(i) pocket and the newly discovered c(i) or x heme. We propose a model for Q(i) site function and a role for Arg(214) in plastoquinone binding.

32 CFR 887.7 - Persons separated under other than honorable conditions (undesirable or bad conduct) or...

Code of Federal Regulations, 2010 CFR

2010-07-01

.... However, an official photocopy of the report of separation or certificate of discharge (DD Form 214... written request of the member. (a) On the DD Forms 214 issued before October 1, 1979, the following items...). (b) For DD Forms 214 issued after October 1, 1979, send one copy with the Special Additional...

32 CFR Appendix B to Part 45 - DD Form 214ws

Code of Federal Regulations, 2013 CFR

2013-07-01

... 32 National Defense 1 2013-07-01 2013-07-01 false DD Form 214ws B Appendix B to Part 45 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) Pt. 45, App. B Appendix B to Part...

32 CFR Appendix B to Part 45 - DD Form 214ws

Code of Federal Regulations, 2011 CFR

2011-07-01

... 32 National Defense 1 2011-07-01 2011-07-01 false DD Form 214ws B Appendix B to Part 45 National Defense Department of Defense OFFICE OF THE SECRETARY OF DEFENSE PERSONNEL, MILITARY AND CIVILIAN CERTIFICATE OF RELEASE OR DISCHARGE FROM ACTIVE DUTY (DD FORM 214/5 SERIES) Pt. 45, App. B Appendix B to Part...

47 CFR 90.214 - Transient frequency behavior.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 47 Telecommunication 5 2012-10-01 2012-10-01 false Transient frequency behavior. 90.214 Section 90... PRIVATE LAND MOBILE RADIO SERVICES General Technical Standards § 90.214 Transient frequency behavior... Behavior for Equipment Designed to Operate on 25 kHz Channels t1 4 ±25.0 kHz 5.0 ms 10.0 ms t2 ±12.5 kHz 20...

47 CFR 90.214 - Transient frequency behavior.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 47 Telecommunication 5 2013-10-01 2013-10-01 false Transient frequency behavior. 90.214 Section 90... PRIVATE LAND MOBILE RADIO SERVICES General Technical Standards § 90.214 Transient frequency behavior... Behavior for Equipment Designed to Operate on 25 kHz Channels t1 4 ±25.0 kHz 5.0 ms 10.0 ms t2 ±12.5 kHz 20...

47 CFR 90.214 - Transient frequency behavior.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 47 Telecommunication 5 2014-10-01 2014-10-01 false Transient frequency behavior. 90.214 Section 90... PRIVATE LAND MOBILE RADIO SERVICES General Technical Standards § 90.214 Transient frequency behavior... Behavior for Equipment Designed to Operate on 25 kHz Channels t1 4 ±25.0 kHz 5.0 ms 10.0 ms t2 ±12.5 kHz 20...

47 CFR 90.214 - Transient frequency behavior.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 47 Telecommunication 5 2011-10-01 2011-10-01 false Transient frequency behavior. 90.214 Section 90... PRIVATE LAND MOBILE RADIO SERVICES General Technical Standards § 90.214 Transient frequency behavior... Behavior for Equipment Designed to Operate on 25 kHz Channels t1 4 ±25.0 kHz 5.0 ms 10.0 ms t2 ±12.5 kHz 20...

Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity

PubMed Central

Sun, Weijia; Zhao, Chenyang; Li, Yuheng; Wang, Liang; Nie, Guangjun; Peng, Jiang; Wang, Aiyuan; Zhang, Pengfei; Tian, Weiming; Li, Qi; Song, Jinping; Wang, Cheng; Xu, Xiaolong; Tian, Yanhua; Zhao, Dingsheng; Xu, Zi; Zhong, Guohui; Han, Bingxing; Ling, Shukuan; Chang, Yan-Zhong; Li, Yingxian

2016-01-01

MicroRNAs have an important role in bone homeostasis. However, the detailed mechanism of microRNA-mediated intercellular communication between bone cells remains elusive. Here, we report that osteoclasts secrete microRNA-enriched exosomes, by which miR-214 is transferred into osteoblasts to inhibit their function. In a coculture system, inhibition of exosome formation and secretion prevented miR-214 transportation. Exosomes specifically recognized osteoblasts through the interaction between ephrinA2 and EphA2. In osteoclast-specific miR-214 transgenic mice, exosomes were secreted into the serum, and miR-214 and ephrinA2 levels were elevated. Therefore, these exosomes have an inhibitory role in osteoblast activity. miR-214 and ephrinA2 levels in serum exosomes from osteoporotic patients and mice were upregulated substantially. These exosomes may significantly inhibit osteoblast activity. Inhibition of exosome secretion via Rab27a small interfering RNA prevented ovariectomized-induced osteoblast dysfunction in vivo. Taken together, these findings suggest that exosome-mediated transfer of microRNA plays an important role in the regulation of osteoblast activity. Circulating miR-214 in exosomes not only represents a biomarker for bone loss but could selectively regulate osteoblast function. PMID:27462462

Diurnal variations of (218)Po, (214)Pb, and (214)Po and their effect on atmospheric electrical conductivity in the lower atmosphere at Mysore city, Karnataka State, India.

PubMed

Pruthvi Rani, K S; Paramesh, L; Chandrashekara, M S

2014-12-01

The short-lived radon daughters ((218)Po, (214)Pb, (214)Bi and (214)Po) are natural tracers in the troposphere, in particular near the ground surface. They are electrically charged particles and are chemically reactive. As soon as they are formed they get attached to the aerosol particles of the atmosphere. The behavior of radon daughters is similar to that of aerosols with respect to their growth, transport and removal processes in the atmosphere. The electrical conductivity of the atmosphere is mainly due to the presence of highly mobile ions. Galactic cosmic rays are the main source of ionization in the planetary boundary layer; however, near the surface of the earth, ions are produced mainly by decays of natural radioactive gases emanating from the soil surface and by radiations emitted directly from the surface. Hence the electrical conductivity of air near the surface of the earth is mainly due to radiations emitted by (222)Rn, (218)Po, (214)Pb, (214)Bi and (214)Po, and depends on aerosol concentrations and meteorological parameters. In the present work the diurnal and seasonal variations of radon and its progeny concentrations are studied using Low Level Radon Detection System and Airflow Meter respectively. Atmospheric electrical conductivity of both positive and negative polarities is measured using a Gerdien Condenser. All the measurements were carried out simultaneously at one location in Mysore city (12°N, 76°E), India. The diurnal variation of atmospheric electrical conductivity was found to be similar to that of ion pair production rate estimated from radon and its progeny concentrations with a maximum in the early morning hours and minimum during day time. The annual average concentrations of (222)Rn, (218)Po, (214)Pb, and (214)Po at the study location were found to be 21.46, 10.88, 1.78 and 1.80 Bq m(-3) respectively. The annual average values of positive and negative atmospheric electrical conductivity were found to be 18.1 and 16.6 f S m(-1) respectively. The radon and its progeny concentrations are higher in winter than in summer and rainy season. Copyright © 2014 Elsevier Ltd. All rights reserved.

20 CFR 416.214 - You are disabled and drug addiction or alcoholism is a contributing factor material to the...

Code of Federal Regulations, 2010 CFR

2010-04-01

... 20 Employees' Benefits 2 2010-04-01 2010-04-01 false You are disabled and drug addiction or alcoholism is a contributing factor material to the determination of disability. 416.214 Section 416.214 Employees' Benefits SOCIAL SECURITY ADMINISTRATION SUPPLEMENTAL SECURITY INCOME FOR THE AGED, BLIND, AND DISABLED Eligibility Reasons Why You May Not...

Credit WCT. Original 214" x 21/4" color negative is housed ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

Credit WCT. Original 2-14" x 2-1/4" color negative is housed in the JPL Photography Laboratory, Pasadena, California. This image depicts the tray dryer for "AP" (ammonium perchlorate, an oxidizer). The dryer was heated by a water jacket; insulated pipes appear at left in the view. In the extreme left foreground appears a marble table similar to the tables used for scales in the weighing room of Building E-35. Note the use of gloves, fireresistant coveralls and breathing apparatus by the JPL employee in view (JPL negative no. JPL-10283BC, 27 January 1989) - Jet Propulsion Laboratory Edwards Facility, Oxidizer Dryer Blender Building, Edwards Air Force Base, Boron, Kern County, CA

The N-end rule pathway catalyzes a major fraction of the protein degradation in skeletal muscle

NASA Technical Reports Server (NTRS)

Solomon, V.; Lecker, S. H.; Goldberg, A. L.

1998-01-01

In skeletal muscle, overall protein degradation involves the ubiquitin-proteasome system. One property of a protein that leads to rapid ubiquitin-dependent degradation is the presence of a basic, acidic, or bulky hydrophobic residue at its N terminus. However, in normal cells, substrates for this N-end rule pathway, which involves ubiquitin carrier protein (E2) E214k and ubiquitin-protein ligase (E3) E3alpha, have remained unclear. Surprisingly, in soluble extracts of rabbit muscle, we found that competitive inhibitors of E3alpha markedly inhibited the 125I-ubiquitin conjugation and ATP-dependent degradation of endogenous proteins. These inhibitors appear to selectively inhibit E3alpha, since they blocked degradation of 125I-lysozyme, a model N-end rule substrate, but did not affect the degradation of proteins whose ubiquitination involved other E3s. The addition of several E2s or E3alpha to the muscle extracts stimulated overall proteolysis and ubiquitination, but only the stimulation by E3alpha or E214k was sensitive to these inhibitors. A similar general inhibition of ubiquitin conjugation to endogenous proteins was observed with a dominant negative inhibitor of E214k. Certain substrates of the N-end rule pathway are degraded after their tRNA-dependent arginylation. We found that adding RNase A to muscle extracts reduced the ATP-dependent proteolysis of endogenous proteins, and supplying tRNA partially restored this process. Finally, although in muscle extracts the N-end rule pathway catalyzes most ubiquitin conjugation, it makes only a minor contribution to overall protein ubiquitination in HeLa cell extracts.

Global DNA methylation analysis reveals miR-214-3p contributes to cisplatin resistance in pediatric intracranial nongerminomatous malignant germ cell tumors.

PubMed

Hsieh, Tsung-Han; Liu, Yun-Ru; Chang, Ting-Yu; Liang, Muh-Lii; Chen, Hsin-Hung; Wang, Hsei-Wei; Yen, Yun; Wong, Tai-Tong

2018-03-27

Pediatric central nervous system germ cell tumors (CNSGCTs) are rare and heterogeneous neoplasms, which can be divided into germinomas and nongerminomatous germ cell tumors (NGGCTs). NGGCTs are further subdivided into mature teratomas and nongerminomatous malignant GCTs (NGMGCTs). Clinical outcomes suggest that NGMGCTs have poor prognosis and survival and that they require more extensive radiotherapy and adjuvant chemotherapy. However, the mechanisms underlying this difference are still unclear. DNA methylation alteration is generally acknowledged to cause therapeutic resistance in cancers. We hypothesized that the pediatric NGMGCTs exhibit a different genome-wide DNA methylation pattern, which is involved in the mechanism of its therapeutic resistance. We performed methylation and hydroxymethylation DNA immunoprecipitation sequencing, mRNA expression microarray, and small RNA sequencing (smRNA-seq) to determine methylation-regulated genes, including microRNAs (miRNAs). The expression levels of 97 genes and 8 miRNAs were correlated with promoter DNA methylation and hydroxymethylation status, such as the miR-199/-214 cluster, and treatment with DNA demethylating agent 5-aza-2'-deoxycytidine elevated its expression level. Furthermore, smRNA-seq analysis showed 27 novel miRNA candidates with differential expression between germinomas and NGMGCTs. Overexpresssion of miR-214-3p in NCCIT cells leads to reduced expression of the pro-apoptotic protein BCL2-like 11 and induces cisplatin resistance. We interrogated the differential DNA methylation patterns between germinomas and NGMGCTs and proposed a mechanism for chemoresistance in NGMGCTs. In addition, our sequencing data provide a roadmap for further pediatric CNSGCT research and potential targets for the development of new therapeutic strategies.

7 CFR 1220.214 - Term of office.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Soybean Promotion and Research Order Soybean Program Coordinating Committee § 1220.214...

7 CFR 1220.214 - Term of office.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE SOYBEAN PROMOTION, RESEARCH, AND CONSUMER INFORMATION Soybean Promotion and Research Order Soybean Program Coordinating Committee § 1220.214...

15 CFR 904.214 - Stipulations.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES Hearing and Appeal Procedures General § 904.214 Stipulations. The parties may, by stipulation, agree upon any...

15 CFR 904.214 - Stipulations.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE GENERAL REGULATIONS CIVIL PROCEDURES Hearing and Appeal Procedures General § 904.214 Stipulations. The parties may, by stipulation, agree upon any...

Spatial distribution of 214Po ions in the electrostatic collection.

PubMed

Barlas, E; Bayrak, A; Emirhan, E; Haciomeroglu, S; Ozben, C S

2013-10-01

A low cost Si-PIN photodiode-based radon monitor was successfully designed and produced to monitor precursory earthquake indicators in the Northern Anatolian Fault Line. The spatial distribution of (214)Po ions was determined by comparing the 7.69 MeV (214)Po peak in the MCA spectrum and the Geant4 energy distribution of alpha particles at various detector source distances. Copyright © 2013 Elsevier Ltd. All rights reserved.

78 FR 69541 - Labor Certification Process for Logging Employment and Non-H-2A Agricultural Employment

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-20

.... 1182 note); 29 U.S.C. 49k; Pub. L. 109-423, 120 Stat. 2900; 8 CFR 214.2(h)(4)(i); and 8 CFR 214.2(h)(6... 8 CFR 214.2(h)(4)(i). Subpart C--[Removed and Reserved] 0 2. Remove and reserve subpart C...,'' 20 CFR 655.103(c)(4), was to include within the program requirements for temporary employment of...

MiR-214 regulates the function of osteoblast under simulated microgravity by targeting ATF4

NASA Astrophysics Data System (ADS)

Li, Yingxian; Wang, Xiaogang; Li, Qi; Lv, Ke; Wan, Yumin; Li, Yinghui; Bai, Yanqiang

Background: MicroRNAs (miRNAs) are small fragments of single-stranded RNA containing 18-24 nucleotides, and are generated from endogenous transcripts. MicroRNAs function in post-transcriptional gene silencing by targeting the 3'-untranslated region (UTR) of mRNAs, resulting in translational repression. Growing evidence shows that microRNAs (miRNAs) regu-late various developmental and homeostatic events in vertebrates and invertebrates. Osteoblast differentiation is a key step in proper skeletal development and acquisition of bone mass; How-ever, the physiological role of non-coding small RNAs, especially miRNAs, in osteoblast dif-ferentiation remains elusive. Methods: To study the potential involvement of miRNAs in osteoblast differentiation under stimulated microgravity, we analyzed the expression of 20 bone relative miRNAs using real time PCR platform to find particularly miRNAs whose expression is altered during osteoblast differentiation. TargetScan, miRBase and Miranda were used to predict the target gene of candidate miRNA. To investigate whether ATF4 can be directly targeted by miR-214, we engineered luciferase reporters that have either the wild-type 3'UTRs of these genes, or the mutant UTRs with a 6 base pair (bp) deletion in the target sites. Lastly, to address the in vivo role of miR-214 in bone formation, tail suspension mice model was used to simulate the change of osteoblast function and bone loss. Results: Recent studies have sug-gested that miRNAs might play a role in osteoblast differentiation and bone formation. Here, we identify miR-214 in MC3T3-E1 cells, which is a primary mouse osteoblasts cell line, to promote osteoblast differentiation by repressing Activating Transcription Factor4 (ATF4) ex-pression at the posttranscriptional level. What is more, miR-214 was found to be transcribed in C2C12 cells during bone morphogenetic protein 2-induced (BMP2-induced) osteogenesis, and overexpression of miR-214 attenuated BMP2-induced osteoblastogenesis, whereas inhibition of miR-214 expression enhanced this progress. The levels of miR-214 increased dramatically in tail suspension mice. Conclusions: Thus, our studies show that miR-214 plays an important role in osteoblast differentiation by targeting ATF4 under stimulated microgravity induced bone loss and contributes to osteoporosis via its effect on osteoblasts.

7 CFR 1280.214 - Refund escrow accounts.

Code of Federal Regulations, 2010 CFR

2010-01-01

... AGREEMENTS AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE LAMB PROMOTION, RESEARCH, AND INFORMATION ORDER Lamb Promotion, Research, and Information Order Expenses § 1280.214 Refund escrow accounts...

The α-MSH analogue AP214 attenuates rise in pulmonary pressure and fall in ejection fraction in lipopolysaccharide-induced systemic inflammatory response syndrome in pigs.

PubMed

Kristensen, Jens; Jonassen, Thomas E N; Rehling, Michael; Tønnesen, Else; Sloth, Erik; Nielsen, Søren; Frøkiaer, Jørgen

2011-01-01

The effect of an α-melanocyte stimulating hormone (α-MSH) analogue (AP214) on experimentally endotoxin-induced systemic inflammatory response syndrome (SIRS) was studied, because α-MSH in rodent models has shown promise in attenuating inflammatory response markers and associated organ damage in SIRS. SIRS is associated with considerable morbidity and mortality. Consequently, new treatment modalities are still warranted to address the different aspects of the pathophysiological process. SIRS was induced by lipopolysaccharide (LPS) (Escherichia coli endotoxin) infusion in anaesthetized Danish Landrace pigs (20-25 kg). The pigs received an α-MSH analogue (AP214) or saline as a bolus at the initiation of the LPS infusion. The hemodynamic response was registered as well as echocardiographic indices of left ventricular function. The cardiovascular response was recorded together with echocardiographic indices of left ventricular function in control and in intervention animals. AP214 reduced the early peak in pulmonary pressure and pulmonary vascular resistance by approximately 33%. Furthermore, AP214 prevented the decline in left ventricular fractional shortening as observed in the control group. Mean change and standard deviation in fractional shortening (ΔFS) in control group: - 7·3 (4·7), AP214 (low dose): 0·9 (8·2) and AP214 (high dose) 4·1 (6·0), P < 0·05 for both intervention groups versus control. In the porcine model, the peak increase in pulmonary pressure was attenuated, and the LPS-induced decline in left ventricular function was prevented. © 2010 The Authors. Clinical Physiology and Functional Imaging © 2010 Scandinavian Society of Clinical Physiology and Nuclear Medicine.

Polaribacter tangerinus sp. nov., isolated from sediment in a sea cucumber culture pond.

PubMed

Han, Ji-Ru; Wang, Kan; Zhang, Jing; Chen, Guan-Jun; Du, Zong-Jun

2017-11-01

A Gram-stain-negative, aerobic, non-motile, coccoid, ovoid or rod-shaped, and orange-pigmented bacterial strain, designated WS2-14 T , was isolated from sediment collected from a sea cucumber culture pond located in Rongcheng, Shandong province, PR China (122° 14' E, 36° 54' N). Strain WS2-14 T grew optimally at 28 °C and pH 7.0-7.5, and was able to tolerate salt concentrations of 0.5-6.0 % (w/v) NaCl. Strain WS2-14 T was characterized chemotaxonomically as possessing menaquinone-6 (MK-6) as the major respiratory quinone, as well as iso-C13 : 0, iso-C15 : 0 3-OH and iso-C15 : 0 as the predominant fatty acids. The DNA G+C content of strain WS2-14 T was 31.2 mol%. The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid and three unidentified lipids. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain WS2-14 T was phylogenetically related to members of the genus Polaribacter and was closely related to Polaribacter haliotis, Polaribacter atrinae and Polaribacter sejongensis with 97.7, 97.4 and 97.0 % sequence similarities, respectively. On the basis of its phenotypic and chemotaxonomic properties, as well as phylogenetic analyses, strain WS2-14 T was considered to represent a novel species of the genus Polaribacter, for which the name Polaribactertangerinus sp. nov. is proposed. The type strain is WS2-14 T (=KCTC 52275 T =MCCC 1H00163 T ).

Heterogeneous patterns of amplification of the NUP214-ABL1 fusion gene in T-cell acute lymphoblastic leukemia.

PubMed

Graux, C; Stevens-Kroef, M; Lafage, M; Dastugue, N; Harrison, C J; Mugneret, F; Bahloula, K; Struski, S; Grégoire, M J; Nadal, N; Lippert, E; Taviaux, S; Simons, A; Kuiper, R P; Moorman, A V; Barber, K; Bosly, A; Michaux, L; Vandenberghe, P; Lahortiga, I; De Keersmaecker, K; Wlodarska, I; Cools, J; Hagemeijer, A; Poirel, H A

2009-01-01

Episomes with the NUP214-ABL1 fusion gene have been observed in 6% of T-ALL. In this multicentric study we collected 27 cases of NUP214-ABL1-positive T-ALL. Median age was 15 years with male predominance. Outcome was poor in 12 patients. An associated abnormality involving TLX1 or TLX3 was found in all investigated cases. Fluorescent in situ hybridization revealed a heterogeneous pattern of NUP214-ABL1 amplification. Multiple episomes carrying the fusion were detected in 24 patients. Episomes were observed in a significant number of nuclei in 18 cases, but in only 1-5% of nuclei in 6. In addition, intrachromosomal amplification (small hsr) was identified either as the only change or in association with episomes in four cases and two T-ALL cell lines (PEER and ALL-SIL). One case showed insertion of apparently non-amplified NUP214-ABL1 sequences at 14q12. The amplified sequences were analyzed using array-based CGH.These findings confirm that the NUP214-ABL1 gene requires amplification for oncogenicity; it is part of a multistep process of leukemogenesis; and it can be a late event present only in subpopulations. Data also provide in vivo evidence for a model of episome formation, amplification and optional reintegration into the genome. Implications for the use of kinase inhibitors are discussed.

Evaluation of drought response of two poplar clones (Populus x canadensis Monch 'I-214' and P. deltoides Marsh. 'Dvina') through high resolution analysis of stem growth.

PubMed

Giovannelli, Alessio; Deslauriers, Annie; Fragnelli, Giuseppe; Scaletti, Luciano; Castro, Gaetano; Rossi, Sergio; Crivellaro, Alan

2007-01-01

Different irrigation effects on stem radius variation (DeltaR) and maximum daily shrinkage (MDS) in Populus deltoides 'Dvina' and Populusxcanadensis 'I-214' were studied to assess differences in drought tolerance between clones. One-year-old trees growing in concrete tanks were submitted to two irrigation regimes (natural rainfall and irrigation) from 24 June to 10 August, and DeltaR was monitored by automatic point dendrometers. Independently of the irrigation regime, 'Dvina' showed a higher stem radial increment than 'I-214'. In both clones, the first response to changed soil water content was a significant increase in MDS, whilst DeltaR decreased about 20 d later when pre-dawn leaf water potential (Psipd) dropped below -0.4 MPa. However, they displayed different strategies to overcome drought. 'Dvina' maintained a positive DeltaR for longer than 'I-214', which had lower leaf Psipd and greater leaf abscission at the end of the drought period. After irrigation resumed, 'Dvina' showed a higher capacity to restore stem growth. 'I-214' was probably unable to recover secondary growth because of higher leaf abscission during drought stress and the production of newly expanded leaves during recovery. It is concluded that the larger radial growth of 'Dvina' derived from a better water use (carbon uptake versus water loss) than 'I-214' under limited water availability.

31 CFR 800.214 - Foreign government-controlled transaction.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (Continued) OFFICE OF INVESTMENT SECURITY, DEPARTMENT OF THE TREASURY REGULATIONS PERTAINING TO MERGERS, ACQUISITIONS, AND TAKEOVERS BY FOREIGN PERSONS Definitions § 800.214 Foreign government-controlled transaction...

49 CFR 214.5 - Responsibility for compliance.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY General § 214.5 Responsibility for compliance. Any... railroad; a manager, supervisor, official, or other employee or agent of a railroad; any owner...

Absolute quantification of regional cerebral glucose utilization in mice by 18F-FDG small animal PET scanning and 2-14C-DG autoradiography.

PubMed

Toyama, Hiroshi; Ichise, Masanori; Liow, Jeih-San; Modell, Kendra J; Vines, Douglass C; Esaki, Takanori; Cook, Michelle; Seidel, Jurgen; Sokoloff, Louis; Green, Michael V; Innis, Robert B

2004-08-01

The purpose of this study was to evaluate the feasibility of absolute quantification of regional cerebral glucose utilization (rCMR(glc)) in mice by use of (18)F-FDG and a small animal PET scanner. rCMR(glc) determined with (18)F-FDG PET was compared with values determined simultaneously by the autoradiographic 2-(14)C-DG method. In addition, we compared the rCMR(glc) values under isoflurane, ketamine and xylazine anesthesia, and awake states. Immediately after injection of (18)F-FDG and 2-(14)C-DG into mice, timed arterial samples were drawn over 45 min to determine the time courses of (18)F-FDG and 2-(14)C-DG. Animals were euthanized at 45 min and their brain was imaged with the PET scanner. The brains were then processed for 2-(14)C-DG autoradiography. Regions of interest were manually placed over cortical regions on corresponding coronal (18)F-FDG PET and 2-(14)C-DG autoradiographic images. rCMR(glc) values were calculated for both tracers by the autoradiographic 2-(14)C-DG method with modifications for the different rate and lumped constants for the 2 tracers. Average rCMR(glc) values in cerebral cortex with (18)F-FDG PET under normoglycemic conditions (isoflurane and awake) were generally lower (by 8.3%) but strongly correlated with those of 2-(14)C-DG (r(2) = 0.95). On the other hand, under hyperglycemic conditions (ketamine/xylazine) average cortical rCMR(glc) values with (18)F-FDG PET were higher (by 17.3%) than those with 2-(14)C-DG. Values for rCMR(glc) and uptake (percentage injected dose per gram [%ID/g]) with (18)F-FDG PET were significantly lower under both isoflurane and ketamine/xylazine anesthesia than in the awake mice. However, the reductions of rCMR(glc) were markedly greater under isoflurane (by 57%) than under ketamine and xylazine (by 19%), whereas more marked reductions of %ID/g were observed with ketamine/xylazine (by 54%) than with isoflurane (by 37%). These reverse differences between isoflurane and ketamine/xylazine may be due to competitive effect of (18)F-FDG and glucose uptake to the brain under hyperglycemia. We were able to obtain accurate absolute quantification of rCMR(glc) with mouse (18)F-FDG PET imaging as confirmed by concurrent use of the autoradiographic 2-(14)C-DG method. Underestimation of rCMR(glc) by (18)F-FDG in normoglycemic conditions may be due to partial-volume effects. Computation of rCMR(glc) from (18)F-FDG data in hyperglycemic animals may require, however, alternative rate and lumped constants for (18)F-FDG.

49 CFR 214.321 - Exclusive track occupancy.

Code of Federal Regulations, 2011 CFR

2011-10-01

..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.321 Exclusive track... printed document directly, by relay through a designated employee, in a data transmission, or by oral...

49 CFR 214.321 - Exclusive track occupancy.

Code of Federal Regulations, 2010 CFR

2010-10-01

..., DEPARTMENT OF TRANSPORTATION RAILROAD WORKPLACE SAFETY Roadway Worker Protection § 214.321 Exclusive track... printed document directly, by relay through a designated employee, in a data transmission, or by oral...

49 CFR 585.71 - Scope.

Code of Federal Regulations, 2011 CFR

2011-10-01

... OF TRANSPORTATION (CONTINUED) PHASE-IN REPORTING REQUIREMENTS Side Impact Protection Phase-in... requirements of S7 of Standard No. 214, Side impact protection (49 CFR 571.214), and the vehicle-to-pole test...

49 CFR 585.71 - Scope.

Code of Federal Regulations, 2013 CFR

2013-10-01

... OF TRANSPORTATION (CONTINUED) PHASE-IN REPORTING REQUIREMENTS Side Impact Protection Phase-in... requirements of S7 of Standard No. 214, Side impact protection (49 CFR 571.214), and the vehicle-to-pole test...

49 CFR 585.71 - Scope.

Code of Federal Regulations, 2014 CFR

2014-10-01

... OF TRANSPORTATION (CONTINUED) PHASE-IN REPORTING REQUIREMENTS Side Impact Protection Phase-in... requirements of S7 of Standard No. 214, Side impact protection (49 CFR 571.214), and the vehicle-to-pole test...

49 CFR 585.71 - Scope.

Code of Federal Regulations, 2012 CFR

2012-10-01

... OF TRANSPORTATION (CONTINUED) PHASE-IN REPORTING REQUIREMENTS Side Impact Protection Phase-in... requirements of S7 of Standard No. 214, Side impact protection (49 CFR 571.214), and the vehicle-to-pole test...

36 CFR 21.4 - Registration of physicians.

Code of Federal Regulations, 2013 CFR

2013-07-01

... INTERIOR HOT SPRINGS NATIONAL PARK; BATHHOUSE REGULATIONS § 21.4 Registration of physicians. Physicians desiring to prescribe the thermal waters of Hot Springs National Park must first be registered at the...

36 CFR 21.4 - Registration of physicians.

Code of Federal Regulations, 2012 CFR

2012-07-01

... INTERIOR HOT SPRINGS NATIONAL PARK; BATHHOUSE REGULATIONS § 21.4 Registration of physicians. Physicians desiring to prescribe the thermal waters of Hot Springs National Park must first be registered at the...

36 CFR 21.4 - Registration of physicians.

Code of Federal Regulations, 2011 CFR

2011-07-01

... INTERIOR HOT SPRINGS NATIONAL PARK; BATHHOUSE REGULATIONS § 21.4 Registration of physicians. Physicians desiring to prescribe the thermal waters of Hot Springs National Park must first be registered at the...

36 CFR 21.4 - Registration of physicians.

Code of Federal Regulations, 2014 CFR

2014-07-01

... INTERIOR HOT SPRINGS NATIONAL PARK; BATHHOUSE REGULATIONS § 21.4 Registration of physicians. Physicians desiring to prescribe the thermal waters of Hot Springs National Park must first be registered at the...

36 CFR 21.4 - Registration of physicians.

Code of Federal Regulations, 2010 CFR

2010-07-01

... INTERIOR HOT SPRINGS NATIONAL PARK; BATHHOUSE REGULATIONS § 21.4 Registration of physicians. Physicians desiring to prescribe the thermal waters of Hot Springs National Park must first be registered at the...

47 CFR 214.6 - Postattack procedures and actions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... PROCEDURES FOR THE USE AND COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.6 Postattack... Radio Spectrum and the Government Master File; (2) Accomplish such coordination as appropriate and...

Lifetime measurements of 214Po and 212Po with the CTF liquid scintillator detector at LNGS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bellini, G.; Benziger, J.; Bick, D.

2013-07-01

We have studied the alpha decays of 214Po into 210Pb and of 212Po into 208Pb tagged by the coincidence with the preceding beta decays from 214Bi and 212Bi, respectively. The employed 222Rn, 232Th, and 220Rn sources were sealed inside quartz vials and inserted in the Counting Test Facility at the underground Gran Sasso National Laboratory in Italy. We find that the mean lifetime of 214Po is (236.00 +- 0.42(stat) +- 0.15(syst)) \\mu s and that of 212Po is (425.1 +- 0.9(stat) +- 1.2(syst)) ns. Our results, obtained from data with signal-to-background ratio larger than 1000, reduce the overall uncertainties andmore » are compatible with previous measurements.« less

Identification of side- and shear-dependent microRNAs regulating porcine aortic valve pathogenesis

NASA Astrophysics Data System (ADS)

Rathan, Swetha; Ankeny, Casey J.; Arjunon, Sivakkumar; Ferdous, Zannatul; Kumar, Sandeep; Fernandez Esmerats, Joan; Heath, Jack M.; Nerem, Robert M.; Yoganathan, Ajit P.; Jo, Hanjoong

2016-05-01

Aortic valve (AV) calcification is an inflammation driven process that occurs preferentially in the fibrosa. To explore the underlying mechanisms, we investigated if key microRNAs (miRNA) in the AV are differentially expressed due to disturbed blood flow (oscillatory shear (OS)) experienced by the fibrosa compared to the ventricularis. To identify the miRNAs involved, endothelial-enriched RNA was isolated from either side of healthy porcine AVs for microarray analysis. Validation using qPCR confirmed significantly higher expression of 7 miRNAs (miR-100, -130a, -181a/b, -199a-3p, -199a-5p, and -214) in the fibrosa versus the ventricularis. Upon bioinformatics analysis, miR-214 was selected for further investigation using porcine AV leaflets in an ex vivo shear system. Fibrosa and ventricularis sides were exposed to either oscillatory or unidirectional pulsatile shear for 2 days and 3 & 7 days in regular and osteogenic media, respectively. Higher expression of miR-214, increased thickness of the fibrosa, and calcification was observed when the fibrosa was exposed to OS compared to the ventricularis. Silencing of miR-214 by anti-miR-214 in whole AV leaflets with the fibrosa exposed to OS significantly increased the protein expression of TGFβ1 and moderately increased collagen content but did not affect AV calcification. Thus, miR-214 is identified as a side- and shear-dependent miRNA that regulates key mechanosensitive gene in AV such as TGFβ1.

Childhood lead poisoning associated with traditional Chinese medicine: a case report and the subsequent lead source inquiry.

PubMed

Lin, Guo-zhen; Wu, Fei; Yan, Chong-huai; Li, Ke; Liu, Xiang-yi

2012-07-11

Traditional medicines or ethnic remedies, such as Ayurvedic drug of India, have been reported in numerous cases to be one of the main exposure risks for severe lead poisoning. We describe the poisoning of 2 young children from a Chinese family in Guangzhou as a result of the use of traditional Chinese medicine instead of baby powder. A 3-y-old boy with blood lead levels (BLLs) of 303 μg/l and his 6-month-old sister with BLLs of 385 μg/l were hospitalized. Laboratory tests showed that the powder, which was purchased in Ganzhou during a family visit, contained a lead concentration of 214,000 mg/kg. A subsequent inspection revealed that the lead contamination was in fact achieved by the addition of Hongdan to talcum. In Ganzhou, it was a popular practice for young children to apply this leaded powder on their skins in order to treat and prevent dermatitis. We sampled 16 Hongdans and observed an average lead content of 817,000 mg/kg that was comprised mainly of lead tetraoxide (Pb(3)O(4)). Lead tetraoxide is also an accessible raw material for paint and battery industries. The health authority of China should reevaluate the safety of such traditional remedy and weigh its toxicity versus its potential benefits. Clinicians should be aware of this leaded powder when treating a lead poisoning case that shows no explainable cause. Copyright © 2012 Elsevier B.V. All rights reserved.

32 CFR 881.8 - Disposition of documents.

Code of Federal Regulations, 2010 CFR

2010-07-01

... documents. (a) File a copy of the application, supporting evidence, and DD Form 214 in the Master Personnel... cases. Send copies of DD Form 214 to: (1) The applicant. (2) The Veterans' Administration. (3) HQ AFPC...

214. RUSTIC BUS SHELTER, GUARDRAILS AND LAMP POST BELLE HAVEN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

214. RUSTIC BUS SHELTER, GUARDRAILS AND LAMP POST BELLE HAVEN BUS STOP WIDENING, 1932. - George Washington Memorial Parkway, Along Potomac River from McLean to Mount Vernon, VA, Mount Vernon, Fairfax County, VA

47 CFR 214.4 - Planned actions.

Code of Federal Regulations, 2013 CFR

2013-10-01

... COORDINATION OF THE RADIO SPECTRUM DURING A WARTIME EMERGENCY § 214.4 Planned actions. (a) Whenever it is... the use of the radio spectrum in areas of active combat, where such control is necessary to the...

26 CFR 2.1-4 - Application to establish fund.

Code of Federal Regulations, 2011 CFR

2011-04-01

... (CONTINUED) MARITIME CONSTRUCTION RESERVE FUND § 2.1-4 Application to establish fund. Any person claiming to... Administration for permission to establish a construction reserve fund. The application shall be in such form and...

26 CFR 2.1-4 - Application to establish fund.

Code of Federal Regulations, 2012 CFR

2012-04-01

... (CONTINUED) MARITIME CONSTRUCTION RESERVE FUND § 2.1-4 Application to establish fund. Any person claiming to... Administration for permission to establish a construction reserve fund. The application shall be in such form and...

26 CFR 2.1-4 - Application to establish fund.

Code of Federal Regulations, 2013 CFR

2013-04-01

... (CONTINUED) MARITIME CONSTRUCTION RESERVE FUND § 2.1-4 Application to establish fund. Any person claiming to... Administration for permission to establish a construction reserve fund. The application shall be in such form and...

26 CFR 2.1-4 - Application to establish fund.

Code of Federal Regulations, 2014 CFR

2014-04-01

... (CONTINUED) MARITIME CONSTRUCTION RESERVE FUND § 2.1-4 Application to establish fund. Any person claiming to... Administration for permission to establish a construction reserve fund. The application shall be in such form and...

26 CFR 2.1-4 - Application to establish fund.

Code of Federal Regulations, 2010 CFR

2010-04-01

... (CONTINUED) MARITIME CONSTRUCTION RESERVE FUND § 2.1-4 Application to establish fund. Any person claiming to... Administration for permission to establish a construction reserve fund. The application shall be in such form and...

Measurement of the {sup 214}Po half-life by the DEVIS track setup

DOE Office of Scientific and Technical Information (OSTI.GOV)

Belov, V. A.; Brakhman, E. V.; Zeldovich, O. Ya.

2013-04-15

Measurement of the {sup 214}Po half-life with the DEVIS track setup at the Institute of Theoretical and Experimental Physics (ITEP, Moscow) by means of a procedure based on determining lifetimes of individual nuclei is described. The value obtained for the {sup 214}Po half-life is 163.8 {+-} 3.0 Micro-Sign s. The possibility of reaching the accuracy of the measurements that is required for testing the statement that the decay of some nuclei has a nonexponential character and the source intensity necessary for this are discussed.

Nonlinear Large Displacement and Moderate Rotational Characteristics of Composite Beams Incorporating Transverse Shear Strain

DTIC Science & Technology

1992-12-01

4-1 iii Page Appendix A. Qj,,H,L,S,k, N ,I 2 . . . . . . . . . . . . . . . . . . . . . . . A-1 Appendix B...2-14 R .................................................... 2-14 N ...2-17 D . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-18 N

76 FR 12307 - Texas: Final Authorization of State-initiated Changes and Incorporation by Reference of State...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-07

...: Send written comments to Alima Patterson, Region 6, Regional Authorization Coordinator, or Julia Banks... Federal Register. FOR FURTHER INFORMATION CONTACT: Alima Patterson, (214) 665-8533 and Julia Banks (214...

NGO Partnerships in Using Ecotourism for Conservation: Systematic Review and Meta-Analysis.

PubMed

Romero-Brito, Tania P; Buckley, Ralf C; Byrne, Jason

2016-01-01

We analyse 214 cases worldwide where non-governmental organisations (NGOs) use ecotourism for conservation. Other stakeholders in these initiatives include local communities, the private sector, and government agencies. Stakeholder relationships determine NGO roles and project management structures and governance. We classified cases into 10 structural categories based on the initiating stakeholder and the NGO role, and used these categories to analyze geographic patterns and success factors. Most of the 214 cases are community-based (~170; 79%); most are in developing countries (190; 89%); and most are in protected areas (196; 91%). Frequencies of structural categories differ between continents. More cases in Latin America and Asia are initiated by NGOs and local communities, and more in Africa by the private sector. Case-study authors used a range of economic, socio-cultural and environmental criteria to judge whether projects were successful. At global scale, we found no significant association between project success and the involvement of private tourism entrepreneurs. Projects involving either local or international NGOs had higher success rates than those that involved both simultaneously. Future research could adopt political ecology approaches to examine: the factors that lead NGOs to adopt ecotourism enterprises; their internal decision-making processes and strategies; their interactions with the stakeholders involved; and their conservation goals and outcomes.

NGO Partnerships in Using Ecotourism for Conservation: Systematic Review and Meta-Analysis

PubMed Central

Romero-Brito, Tania P.; Byrne, Jason

2016-01-01

We analyse 214 cases worldwide where non-governmental organisations (NGOs) use ecotourism for conservation. Other stakeholders in these initiatives include local communities, the private sector, and government agencies. Stakeholder relationships determine NGO roles and project management structures and governance. We classified cases into 10 structural categories based on the initiating stakeholder and the NGO role, and used these categories to analyze geographic patterns and success factors. Most of the 214 cases are community-based (~170; 79%); most are in developing countries (190; 89%); and most are in protected areas (196; 91%). Frequencies of structural categories differ between continents. More cases in Latin America and Asia are initiated by NGOs and local communities, and more in Africa by the private sector. Case-study authors used a range of economic, socio-cultural and environmental criteria to judge whether projects were successful. At global scale, we found no significant association between project success and the involvement of private tourism entrepreneurs. Projects involving either local or international NGOs had higher success rates than those that involved both simultaneously. Future research could adopt political ecology approaches to examine: the factors that lead NGOs to adopt ecotourism enterprises; their internal decision-making processes and strategies; their interactions with the stakeholders involved; and their conservation goals and outcomes. PMID:27893800

10 CFR 72.214 - List of approved spent fuel storage casks.

Code of Federal Regulations, 2010 CFR

2010-01-01

... STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN CLASS C WASTE General License for Storage of Spent Fuel at Power Reactor Sites § 72.214 List of approved spent...

10 CFR 72.214 - List of approved spent fuel storage casks.

Code of Federal Regulations, 2011 CFR

2011-01-01

... STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN CLASS C WASTE General License for Storage of Spent Fuel at Power Reactor Sites § 72.214 List of approved spent...

24 CFR 214.311 - Funding.

Code of Federal Regulations, 2010 CFR

2010-04-01

... Internet or other electronic media. (b) Local funding sources. HUD recommends that approved agencies seek and secure funding from funding sources that may include local and state governments, private... violate the provisions regarding conflicts of interest described in § 214.303(e). ...