Mounting for Fabrication, Metrology, and Assembly of Full Shell Grazing Incidence Optics

NASA Technical Reports Server (NTRS)

Roche, Jacqueline M.; Gubarev, Mikhail V.; O'Dell, Stephen L.; Kolodziejczak, Jeffery; Weisskopf, Martin C.; Ramsey, Brian D.; Elsner, Ronald F.

2014-01-01

Future x-ray telescopes will likely require lightweight mirrors to attain the large collecting areas needed to accomplish the science objectives. Understanding and demonstrating processes now is critical to achieving sub-arcsecond performance in the future. Consequently, designs not only of the mirrors but of fixtures for supporting them during fabrication, metrology, handling, assembly, and testing must be adequately modeled and verified. To this end, MSFC is using finite-element modeling to study the effects of mounting on full-shell grazing-incidence mirrors, during all processes leading to flight mirror assemblies. Here we report initial results of this study.

FUEL ASSEMBLY SHAKER TEST SIMULATION

DOE Office of Scientific and Technical Information (OSTI.GOV)

Klymyshyn, Nicholas A.; Sanborn, Scott E.; Adkins, Harold E.

This report describes the modeling of a PWR fuel assembly under dynamic shock loading in support of the Sandia National Laboratories (SNL) shaker test campaign. The focus of the test campaign is on evaluating the response of used fuel to shock and vibration loads that a can occur during highway transport. Modeling began in 2012 using an LS-DYNA fuel assembly model that was first created for modeling impact scenarios. SNL’s proposed test scenario was simulated through analysis and the calculated results helped guide the instrumentation and other aspects of the testing. During FY 2013, the fuel assembly model was refinedmore » to better represent the test surrogate. Analysis of the proposed loads suggested the frequency band needed to be lowered to attempt to excite the lower natural frequencies of the fuel assembly. Despite SNL’s expansion of lower frequency components in their five shock realizations, pretest predictions suggested a very mild dynamic response to the test loading. After testing was completed, one specific shock case was modeled, using recorded accelerometer data to excite the model. Direct comparison of predicted strain in the cladding was made to the recorded strain gauge data. The magnitude of both sets of strain (calculated and recorded) are very low, compared to the expected yield strength of the Zircaloy-4 material. The model was accurate enough to predict that no yielding of the cladding was expected, but its precision at predicting micro strains is questionable. The SNL test data offers some opportunity for validation of the finite element model, but the specific loading conditions of the testing only excite the fuel assembly to respond in a limited manner. For example, the test accelerations were not strong enough to substantially drive the fuel assembly out of contact with the basket. Under this test scenario, the fuel assembly model does a reasonable job of approximating actual fuel assembly response, a claim that can be verified through direct comparison of model results to recorded test results. This does not offer validation for the fuel assembly model in all conceivable cases, such as high kinetic energy shock cases where the fuel assembly might lift off the basket floor to strike to basket ceiling. This type of nonlinear behavior was not witnessed in testing, so the model does not have test data to be validated against.a basis for validation in cases that substantially alter the fuel assembly response range. This leads to a gap in knowledge that is identified through this modeling study. The SNL shaker testing loaded a surrogate fuel assembly with a certain set of artificially-generated time histories. One thing all the shock cases had in common was an elimination of low frequency components, which reduces the rigid body dynamic response of the system. It is not known if the SNL test cases effectively bound all highway transportation scenarios, or if significantly greater rigid body motion than was tested is credible. This knowledge gap could be filled through modeling the vehicle dynamics of a used fuel conveyance, or by collecting acceleration time history data from an actual conveyance under highway conditions.« less

Satellite Servicing Capabilities Office Testing

NASA Technical Reports Server (NTRS)

Sanders, Sean

2015-01-01

While at the KSC, I was given the opportunity of assisting the Satellite Servicing Capabilities Office (SSCO) specifically the Propellant Transfer System (PTS) lead by my mentor, Brian Nufer. While waiting to test different components in the PTS, I was able to assist with testing for the Hose Management Assembly (HMA) and was able to work on a simulation in Labview. For the HMA, I was able to help with testing of a coating as well as to help test the durability of the pinch rollers in space. In Labview, I experimented with building a simulation for the PTS, to show where fluids and gases were flowing depending on which valves in the PTS were opened. Not all of the integrated parts required assembly level testing, which allowed me to test these parts individually by myself and document the results. I was also able to volunteer to assist project NEO, allowing me to gain some knowledge of cryogenic fluid systems.

Dropping in on a Clean Room Webb Test

NASA Image and Video Library

2017-12-08

A crane in a clean room at NASA's Goddard Space Flight Center in Greenbelt, Md., lowers a test mass simulator (center of frame) onto the Ambient Optical Assembly Stand or AOAS to ensure it can support the James Webb Space Telescope's Optical Telescope Element during its assembly. Credit: NASA/Chris Gunn NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

Lead oxide as used in lead acid storage batteries, part two

DOE Office of Scientific and Technical Information (OSTI.GOV)

Orsino, J.A.

1986-06-01

Without oxide controls, the battery manufacturing business can become one of the most confusing and frustrating experiences known. Inexplicable things happen during mixing, pasting and assembly, and testing, in the laboratory or in the field becomes an unhappy event. Almost any oxide of sufficient purity can be processed to make a good battery, but the characteristics must be known to be able to process it right, and once the process has been established, the oxide must be uniformly made to make the resulting batteries uniformly good. Fortunately, the tests required to assure uniformity are few, and simple to perform. Assumingmore » pure pig lead from primary sources or from carefully refined secondary sources, three tests can tell the whole story. These tests are described.« less

76 FR 72686 - 36(b)(1) Arms Sales Notification

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-25

... Electromagnetic Aircraft Launch System/Advanced Arresting Gear (EMALS/AAG). The EMALS long lead sub-assemblies... United Kingdom--Electromagnetic Aircraft Launch System Long Lead Sub- Assemblies The Government of the United Kingdom (UK) has requested the long lead sub-assemblies for the Electromagnetic Aircraft Launch...

About White Sands Missile Range

NASA Technical Reports Server (NTRS)

1991-01-01

Information on the White Sands Missile Range is given in viewgraph form. Navy programs, test sites, rocket programs, research rockets' booster capacity, current boost capabilities, ordnance and payload assembly areas, commercial space launch history and agreements, and lead times are among the topics covered.

Dynamic fatigue performance of implant-abutment assemblies with different tightening torque values.

PubMed

Xia, Dandan; Lin, Hong; Yuan, Shenpo; Bai, Wei; Zheng, Gang

2014-01-01

Implant-abutment assemblies are usually subject to long-term cyclic loading. To evaluate the dynamic fatigue performance of implant-abutment assemblies with different tightening torque values, thirty implant-abutment assemblies (Zimmer Dental, Carlsbad, CA, USA) were randomly assigned to three tightening groups (24 Ncm; 30 Ncm; 36 Ncm), each consisted of 10 implants. Five specimens from each group were unscrewed, and their reverse torque values recorded. The remaining specimens were subjected to a load between 30 N~300 N at a loading frequency of 15 Hz for 5 × 10(6) cycles. After fatigue tests, residual reverse torque values were recorded if available. In the 24 Ncm tightening group, all the implants fractured at the first outer thread of the implant after fatigue loading, with fatigue crack propagation at the fractured surface showed by SEM observation. For the 30 Ncm and 36 Ncm tightening groups, a statistical significant difference (p<0.05) between the unloaded and loaded groups was revealed. Compared with the unloaded specimens, the specimens went through fatigue loading had decreased reverse torque values. It was demonstrated that insufficient torque will lead to poor fatigue performance of dental implant-abutment assemblies and abutment screws should be tightened to the torque recommended by the manufacturer. It was also concluded that fatigue loading would lead to preload loss.

Development of the Variable Atmosphere Testing Facility for Blow-Down Analysis of the Mars Hopper Prototype

DOE Office of Scientific and Technical Information (OSTI.GOV)

Nathan D. Jerred; Robert C. O'Brien; Steven D. Howe

Recent developments at the Center for Space Nuclear Research (CSNR) on a Martian exploration probe have lead to the assembly of a multi-functional variable atmosphere testing facility (VATF). The VATF has been assembled to perform transient blow-down analysis of a radioisotope thermal rocket (RTR) concept that has been proposed for the Mars Hopper; a long-lived, long-ranged mobile platform for the Martian surface. This study discusses the current state of the VATF as well as recent blow-down testing performed on a laboratory-scale prototype of the Mars Hopper. The VATF allows for the simulation of Mars ambient conditions within the pressure vesselmore » as well as to safely perform blow-down tests through the prototype using CO2 gas; the proposed propellant for the Mars Hopper. Empirical data gathered will lead to a better understanding of CO2 behavior and will provide validation of simulation models. Additionally, the potential of the VATF to test varying propulsion system designs has been recognized. In addition to being able to simulate varying atmospheres and blow-down gases for the RTR, it can be fitted to perform high temperature hydrogen testing of fuel elements for nuclear thermal propulsion.« less

Development of Stable, Low Resistance Solder Joints for a Space-Flight HTS Lead Assemblies

NASA Technical Reports Server (NTRS)

Canavan, Edgar R.; Chiao, Meng; Panashchenko, Lyudmyla; Sampson, Michael

2017-01-01

The solder joints in spaceflight high temperature superconductor (HTS) lead assemblies for certain astrophysics missions have strict constraints on size and power dissipation. In addition, the joints must tolerate years of storage at room temperature, many thermal cycles, and several vibration tests between their manufacture and their final operation on orbit. As reported previously, solder joints between REBCO coated conductors and normal metal traces for the Astro-H mission showed low temperature joint resistance that grew approximately as log time over the course of months. Although the assemblies worked without issue in orbit, for the upcoming X-ray Astrophysics Recovery Mission we are attempting to improve our solder process to give lower, more stable, and more consistent joint resistance. We produce numerous sample joints and measure time- and thermal cycle-dependent resistance, and characterize the joints using x-ray and other analysis tools. For a subset of the joints, we use SEMEDS to try to understand the physical and chemical processes that effect joint behavior.

A leading edge heating array and a flat surface heating array - operation, maintenance and repair manual

NASA Technical Reports Server (NTRS)

1975-01-01

A general description of the leading edge/flat surface heating array is presented along with its components, assembly instructions, installation instructions, operation procedures, maintenance instructions, repair procedures, schematics, spare parts lists, engineering drawings of the array, and functional acceptance test log sheets. The proper replacement of components, correct torque values, step-by-step maintenance instructions, and pretest checkouts are described.

Body of Knowledge (BOK) for Leadless Quad Flat No-Lead/bottom Termination Components (QFN/BTC) Package Trends and Reliability

NASA Technical Reports Server (NTRS)

Ghaffarian, Reza

2014-01-01

Bottom terminated components and quad flat no-lead (BTC/QFN) packages have been extensively used by commercial industry for more than a decade. Cost and performance advantages and the closeness of the packages to the boards make them especially unique for radio frequency (RF) applications. A number of high-reliability parts are now available in this style of package configuration. This report presents a summary of literature surveyed and provides a body of knowledge (BOK) gathered on the status of BTC/QFN and their advanced versions of multi-row QFN (MRQFN) packaging technologies. The report provides a comprehensive review of packaging trends and specifications on design, assembly, and reliability. Emphasis is placed on assembly reliability and associated key design and process parameters because they show lower life than standard leaded package assembly under thermal cycling exposures. Inspection of hidden solder joints for assuring quality is challenging and is similar to ball grid arrays (BGAs). Understanding the key BTC/QFN technology trends, applications, processing parameters, workmanship defects, and reliability behavior is important when judicially selecting and narrowing the follow-on packages for evaluation and testing, as well as for the low risk insertion in high-reliability applications.

Body of Knowledge (BOK) for Leadless Quad Flat No-Lead/Bottom Termination Components (QFN/BTC) Package Trends and Reliability

NASA Technical Reports Server (NTRS)

Ghaffarian, Reza

2014-01-01

Bottom terminated components and quad flat no-lead (BTC/QFN) packages have been extensively used by commercial industry for more than a decade. Cost and performance advantages and the closeness of the packages to the boards make them especially unique for radio frequency (RF) applications. A number of high-reliability parts are now available in this style of package configuration. This report presents a summary of literature surveyed and provides a body of knowledge (BOK) gathered on the status of BTC/QFN and their advanced versions of multi-row QFN (MRQFN) packaging technologies. The report provides a comprehensive review of packaging trends and specifications on design, assembly, and reliability. Emphasis is placed on assembly reliability and associated key design and process parameters because they show lower life than standard leaded package assembly under thermal cycling exposures. Inspection of hidden solder joints for assuring quality is challenging and is similar to ball grid arrays (BGAs). Understanding the key BTC/QFN technology trends, applications, processing parameters, workmanship defects, and reliability behavior is important when judicially selecting and narrowing the follow-on packages for evaluation and testing, as well as for the low risk insertion in high-reliability applications.

Fabrication and assembly of a superconducting undulator for the advanced photon source

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hasse, Quentin; Fuerst, J. D.; Ivanyushenkov, Y.

2014-01-29

A prototype superconducting undulator magnet (SCU0) has been built at the Advanced Photon Source (APS) of Argonne National Laboratory (ANL) and has successfully completed both cryogenic performance and magnetic measurement test programs. The SCU0 closed loop, zero-boil-off cryogenic system incorporates high temperature superconducting (HTS) current leads, cryocoolers, a LHe reservoir supplying dual magnetic cores, and an integrated cooled beam chamber. This system presented numerous challenges in the design, fabrication, and assembly of the device. Aspects of this R and D relating to both the cryogenic and overall assembly of the device are presented here. The SCU0 magnet has been installedmore » in the APS storage ring.« less

Some assembly required: leveraging Web science to understand and enable team assembly

PubMed Central

Contractor, Noshir

2013-01-01

Recent advances on the Web have generated unprecedented opportunities for individuals around the world to assemble into teams. And yet, because of the Web, the nature of teams and how they are assembled has changed radically. Today, many teams are ad hoc, agile, distributed, transient entities that are assembled from a larger primordial network of relationships within virtual communities. These assemblages possess the potential to unleash the high levels of creativity and innovation necessary for productively addressing many of the daunting challenges confronting contemporary society. This article argues that Web science is particularly well suited to help us realize this potential by making a substantial interdisciplinary intellectual investment in (i) advancing theories that explain our socio-technical motivations to form teams, (ii) the development of new analytic methods and models to untangle the unique influences of these motivations on team assembly, (iii) harvesting, curating and leveraging the digital trace data offered by the Web to test our models, and (iv) implementing recommender systems that use insights gleaned from our richer theoretical understanding of the motivations that lead to effective team assembly. PMID:23419854

Some assembly required: leveraging Web science to understand and enable team assembly.

PubMed

Contractor, Noshir

2013-03-28

Recent advances on the Web have generated unprecedented opportunities for individuals around the world to assemble into teams. And yet, because of the Web, the nature of teams and how they are assembled has changed radically. Today, many teams are ad hoc, agile, distributed, transient entities that are assembled from a larger primordial network of relationships within virtual communities. These assemblages possess the potential to unleash the high levels of creativity and innovation necessary for productively addressing many of the daunting challenges confronting contemporary society. This article argues that Web science is particularly well suited to help us realize this potential by making a substantial interdisciplinary intellectual investment in (i) advancing theories that explain our socio-technical motivations to form teams, (ii) the development of new analytic methods and models to untangle the unique influences of these motivations on team assembly, (iii) harvesting, curating and leveraging the digital trace data offered by the Web to test our models, and (iv) implementing recommender systems that use insights gleaned from our richer theoretical understanding of the motivations that lead to effective team assembly.

Development of a 12-Thrust Chamber Kerosene /Oxygen Primary Rocket Sub-System for an Early (1964) Air-Augmented Rocket Ground-Test System

NASA Technical Reports Server (NTRS)

Pryor, D.; Hyde, E. H.; Escher, W. J. D.

1999-01-01

Airbreathing/Rocket combined-cycle, and specifically rocket-based combined- cycle (RBCC), propulsion systems, typically employ an internal engine flow-path installed primary rocket subsystem. To achieve acceptably short mixing lengths in effecting the "air augmentation" process, a large rocket-exhaust/air interfacial mixing surface is needed. This leads, in some engine design concepts, to a "cluster" of small rocket units, suitably arrayed in the flowpath. To support an early (1964) subscale ground-test of a specific RBCC concept, such a 12-rocket cluster was developed by NASA's Marshall Space Flight Center (MSFC). The small primary rockets used in the cluster assembly were modified versions of an existing small kerosene/oxygen water-cooled rocket engine unit routinely tested at MSFC. Following individual thrust-chamber tests and overall subsystem qualification testing, the cluster assembly was installed at the U. S. Air Force's Arnold Engineering Development Center (AEDC) for RBCC systems testing. (The results of the special air-augmented rocket testing are not covered here.) While this project was eventually successfully completed, a number of hardware integration problems were met, leading to catastrophic thrust chamber failures. The principal "lessons learned" in conducting this early primary rocket subsystem experimental effort are documented here as a basic knowledge-base contribution for the benefit of today's RBCC research and development community.

Development and fabrication of a solar cell junction processing system

NASA Technical Reports Server (NTRS)

Kiesling, R.

1981-01-01

The major component fabrication program was completed. Assembly and system testing of the pulsed electron beam annealing machine are described. The design program for the transport reached completion, and the detailed drawings were released for fabrication and procurement of the long lead time components.

Arp2 depletion inhibits sheet-like protrusions but not linear protrusions of fibroblasts and lymphocytes

PubMed Central

Nicholson-Dykstra, Susan M.; Higgs, Henry N.

2009-01-01

The Arp2/3 complex-mediated assembly and protrusion of a branched actin network at the leading edge occurs during cell migration, although some studies suggest it is not essential. In order to test the role of Arp2/3 complex in leading edge protrusion, Swiss 3T3 fibroblasts and Jurkat T cells were depleted of Arp2 and evaluated for defects in cell morphology and spreading efficiency. Arp2-depleted fibroblasts exhibit severe defects in formation of sheet-like protrusions at early time points of cell spreading, with sheet-like protrusions limited to regions along the length of linear protrusions. However, Arp2-depleted cells are able to spread fully after extended times. Similarly, Arp2-depleted Jurkat T lymphocytes exhibit defects in spreading on anti-CD3. Interphase Jurkats in suspension are covered with large ruffle structures, whereas mitotic Jurkats are covered by finger-like linear protrusions. Arp2-depleted Jurkats exhibit defects in ruffle assembly but not in assembly of mitotic linear protrusions. Similarly, Arp2-depletion has no effect on the highly dynamic linear protrusion of another suspended lymphocyte line. We conclude that Arp2/3 complex plays a significant role in assembly of sheet-like protrusions, especially during early stages of cell spreading, but is not required for assembly of a variety of linear actin-based protrusions. PMID:18720401

3. NORTH ELEVATION OF THE HOT BAY, SHOWING RAILROAD TRACKS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. NORTH ELEVATION OF THE HOT BAY, SHOWING RAILROAD TRACKS LEADING TO THE MASSIVE STEEL-LINED CONCRETE ENTRANCE DOOR. PART OF THE INTRICATE HVAC SYSTEM IS WEST (RIGHT) OF THE DOOR. - Nevada Test Site, Engine Maintenance Assembly & Disassembly Facility, Area 25, Jackass Flats, Mercury, Nye County, NV

Development of Lead Free Energy Absorber for Space Shuttle Blast Container

NASA Technical Reports Server (NTRS)

Ingram, T.; Balles, D.; Schricker, A.; Novak, H.

1998-01-01

The Space Shuttle vehicle (SSV) is connected to the mobile launch platform (MLP) by four aft skirt hold down studs on each solid rocket booster (SRB). Prior to lift-off, the frangible nuts inside the aft skirt blast containers (BC) are severed into two nut halves by two pyrotechnic booster cartridges. This action releases the SSV and allows the hold down studs to eject through the aft skirt bore and then down into the MLP. USBI has been tasked to upgrade the BC for two specific reasons; 1. to eliminate lead for environmental concerns, and 2. to reduce the chance of nut recontact with the holddown stud. Nut recontact with the stud has been identified as a likely contributor to stud hangups. This upgrade will replace the lead liner with an aluminum foam material. The aluminum foam used as a energy absorber is a proven design in many other aerospace/defense applications. Additional benefits of using the open cell, energy absorbent aluminum foam in place of the solid lead liner are: A. Lead handling/ exposure, and possible contamination, along with hazardous waste disposal will be eliminated; B. Approximately 200 lbs. weight savings will be contributed to each Space Shuttle flight by using aluminum foam over lead; C. The new aluminum liner is designed to catch all shrapnel from frangible nuts thus virtually eliminating chance of foreign object debris (FOD) exiting the HDP, and causing potential damage to the vehicle; D. Potential of using the lighter aluminum liner over lead, allows for easier assembly and disassembly of blast container elements, also allowing for improvements in safety, operator handling, and efficiency of operations. Six BC firing tests will be required to determine if the new liner material will perform in a way to decrease the chance of stud hangups and enhance the ability of the BC to retain blast debris. Testing will be performed at the Kennedy Space Center (KSC) facility known as the Launch Equipment Test Facility (LETF), and will simulate the SRB hold- down post, with actual BC hardware and pyrotechnics assembled, and then test fired. Initial testing was performed in 1997 using a frangible nut in a static drop test over lead and aluminum foam sheet materials. The aluminum foam showed a dramatic improvement of energy absorption over the lead liner material. Proof-of-Principle testing at the KSC-LETF commenced in May, 1998, and is expected to be completed by June, 1998.

Photovoltaic system with improved AC connections and method of making same

DOEpatents

Cioffi, Philip Michael; Todorovic, Maja Harfman; Herzog, Michael Scott; Korman, Charles Steven; Doherty, Donald M.; Johnson, Neil Anthony

2018-02-13

An alternating current (AC) harness for a photovoltaic (PV) system includes a wire assembly having a first end and a second end, the wire assembly having a plurality of lead wires, and at least one AC connection module positioned at a location along a length of the wire assembly between the first end and the second end. Further, the at least one AC connection module includes a first connection terminal electrically coupled to the plurality of lead wires of the wire assembly and constructed to electrically couple the wire assembly with an output of a first PV module of the PV system. The at least one AC connection module also includes a second connection terminal electrically coupled to the plurality of lead wires of the wire assembly and constructed to electrically couple the wire assembly with an output of a second PV module of the PV system.

Hybrid lead-acid battery with reticulated vitreous carbon as a carrier- and current-collector of negative plate

NASA Astrophysics Data System (ADS)

Czerwiński, A.; Obrębowski, S.; Kotowski, J.; Rogulski, Z.; Skowroński, J.; Bajsert, M.; Przystałowski, M.; Buczkowska-Biniecka, M.; Jankowska, E.; Baraniak, M.; Rotnicki, J.; Kopczyk, M.

Bare reticulated vitreous carbon (RVC) plated electrochemically with thin layer of lead was investigated as a negative plate carrier- and current-collector material for lead-acid batteries. Hybrid flooded single cell lead-acid batteries containing one negative plate based on a new type (RVC or Pb/RVC) of carrier/current-collector and two positive plates based on Pb-Ca grid collectors were assembled and subjected to charge/discharge tests (at 20-h and 1-h discharge rates) and Peukert's dependences determination. The promising results show that application of RVC as carrier- and current-collector in negative plate will significantly increase the specific capacity of lead-acid battery.

Technological development of multispectral filter assemblies for micro bolometer

NASA Astrophysics Data System (ADS)

Le Goff, Roland; Tanguy, François; Fuss, Philippe; Etcheto, Pierre

2017-11-01

Since 2007 Sodern has successfully developed visible and near infrared multispectral filter assemblies for Earth remote sensing imagers. Filter assembly is manufactured by assembling several sliced filter elements (so-called strips), each corresponding to one spectral band. These strips are cut from wafers using a two dimensional accuracy precision process. In the frame of a 2011 R&T preparatory initiative undertaken by the French agency CNES, the filter assembly concept was adapted by Sodern to the long wave infrared spectral band taken into account the germanium substrate, the multilayer bandpass filters and the F-number of the optics. Indeed the current trend in space instrumentation toward more compact uncooled infrared radiometer leads to replace the filter wheel with a multispectral filter assembly mounted directly above the micro bolometer window. The filter assembly was customized to fit the bolometer size. For this development activity we consider a ULIS VGA LWIR micro bolometer with 640 by 480 pixels and 25 microns pixel pitch. The feasibility of the concept and the ability to withstand space environment were investigated and demonstrated by bread boarding activities. The presentation will contain a detailed description of the bolometer and filter assembly design, the stray light modeling analysis assessing the crosstalk between adjacent spectral bands and the results of the manufacturing and environmental tests (damp heat and thermal vacuum cycling).

A trait-based test for habitat filtering: Convex hull volume

USGS Publications Warehouse

Cornwell, W.K.; Schwilk, D.W.; Ackerly, D.D.

2006-01-01

Community assembly theory suggests that two processes affect the distribution of trait values within communities: competition and habitat filtering. Within a local community, competition leads to ecological differentiation of coexisting species, while habitat filtering reduces the spread of trait values, reflecting shared ecological tolerances. Many statistical tests for the effects of competition exist in the literature, but measures of habitat filtering are less well-developed. Here, we present convex hull volume, a construct from computational geometry, which provides an n-dimensional measure of the volume of trait space occupied by species in a community. Combined with ecological null models, this measure offers a useful test for habitat filtering. We use convex hull volume and a null model to analyze California woody-plant trait and community data. Our results show that observed plant communities occupy less trait space than expected from random assembly, a result consistent with habitat filtering. ?? 2006 by the Ecological Society of America.

2009 Community College Futures Assembly Focus: Leading Change--Leading in an Uncertain Environment

ERIC Educational Resources Information Center

Campbell, Dale F.; Morris, Phillip A.

2009-01-01

The Community College Futures Assembly has served as a national, independent policy thinktank since 1995. Its purpose is to articulate the critical issues facing American community colleges and recognize innovative programs. Convening annually in January in Orlando, Florida, the Assembly offers a learning environment where tough questions are…

Design and Analysis of the International X-Ray Observatory Mirror Modules

NASA Technical Reports Server (NTRS)

McClelland, Ryan S.; Carnahan, Timothy M.; Robinson, David W.; Saha, Timo T.

2009-01-01

The Soft X-Ray Telescope (SXT) modules are the fundamental focusing assemblies on NASA's next major X-ray telescope mission, the International X-Ray Observatory (IXO). The preliminary design and analysis of these assemblies has been completed, addressing the major engineering challenges and leading to an understanding of the factors effecting module performance. Each of the 60 modules in the Flight Mirror Assembly (FMA) supports 200-300 densely packed 0.4 mm thick glass mirror segments in order to meet the unprecedented effective area required to achieve the scientific objectives of the mission. Detailed Finite Element Analysis (FEA), materials testing, and environmental testing have been completed to ensure the modules can be successfully launched. Resulting stress margins are positive based on detailed FEA, a large factor of safety, and a design strength determined by robust characterization of the glass properties. FEA correlates well with the results of the successful modal, vibration, and acoustic environmental tests. Deformation of the module due to on-orbit thermal conditions is also a major design driver. A preliminary thermal control system has been designed and the sensitivity of module optical performance to various thermal loads has been determined using optomechanical analysis methods developed for this unique assembly. This design and analysis furthers the goal of building a module that demonstrates the ability to meet IXO requirements, which is the current focus of the IXO FMA technology development team.

The Lead-Lead Oxide Secondary Cell as a Teaching Resource

ERIC Educational Resources Information Center

Smith, Michael J.; Fonseca, Antonio M.; Silva, M. Manuela

2009-01-01

The assembly and use of a laboratory version of a secondary cell based on the lead-lead oxide system is described. The cell is easy to construct, sufficiently robust for student use, and has a conveniently low practical capacity of about 5 mA h. This modest cell capacity allows cell assembly, electrode formation and discharge characterization…

Adhesive bonding and performance testing of bonded wood products

Treesearch

Charles R. Frihart

2005-01-01

Despite the importance of durable wood bonds, the factors that lead to durability are not well understood, and the internal forces exerted upon the bondline are often overlooked. Durability requires that the bonded assembly resist dimensional changes of wood with fluctuation of wood moisture levels. Both bonding and bond breaking steps need to be understood at cellular...

Optimal Assembly of Psychological and Educational Tests.

ERIC Educational Resources Information Center

van der Linden, Wim J.

1998-01-01

Reviews optimal test-assembly literature and introduces the contributions to this special issue. Discusses four approaches to computerized test assembly: (1) heuristic-based test assembly; (2) 0-1 linear programming; (3) network-flow programming; and (4) an optimal design approach. Contains a bibliography of 90 sources on test assembly.…

Coefficients of discharge of fuel-injection nozzles for compression-ignition engines

NASA Technical Reports Server (NTRS)

Gelalles, A G

1932-01-01

This report presents the results of an investigation to determine the coefficients of discharge of nozzles with small, round orifices of the size used with high-speed compression-ignition engines. The injection pressures and chamber back pressures employed were comparable to those existing in compression-ignition engines during injection. The construction of the nozzles was varied to determine the effect of the nozzle design on the coefficient. Tests were also made with nozzles assembled in an automatic injection valve, both with a plain and with a helically grooved stem. It was found that a smooth passage before the orifice is requisite for high flow efficiency. A beveled leading edge before the orifice gave a higher coefficient of discharge than a rounded edge. The results with the nozzles assembled in an automatic injection valve having a plain stem duplicated those with the nozzles assembled at the end of a straight tube of constant diameter. Lower coefficients were obtained with the nozzles assembled in an injection valve having a helically grooved stem. When the coefficients of nozzles of any one geometrical shape were plotted against values of corresponding Reynold's numbers for the orifice diameters and rates of flow tested, it was found that experimental points were distributed along a single curve.

49 CFR 572.162 - Head assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Head assembly and test procedure. 572.162 Section... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.162 Head assembly and test procedure. The head assembly is assembled and tested as specified in 49 CFR 572.122 (Subpart N). ...

49 CFR 572.162 - Head assembly and test procedure.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 7 2010-10-01 2010-10-01 false Head assembly and test procedure. 572.162 Section... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.162 Head assembly and test procedure. The head assembly is assembled and tested as specified in 49 CFR 572.122 (Subpart N). ...

49 CFR 572.162 - Head assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Head assembly and test procedure. 572.162 Section... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.162 Head assembly and test procedure. The head assembly is assembled and tested as specified in 49 CFR 572.122 (Subpart N). ...

49 CFR 572.163 - Neck assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Neck assembly and test procedure. 572.163 Section... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.163 Neck assembly and test procedure. The neck assembly is assembled and tested as specified in 49 CFR 572.123 (Subpart N). ...

49 CFR 572.163 - Neck assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Neck assembly and test procedure. 572.163 Section... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.163 Neck assembly and test procedure. The neck assembly is assembled and tested as specified in 49 CFR 572.123 (Subpart N). ...

49 CFR 572.162 - Head assembly and test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Head assembly and test procedure. 572.162 Section... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.162 Head assembly and test procedure. The head assembly is assembled and tested as specified in 49 CFR 572.122 (Subpart N). ...

49 CFR 572.163 - Neck assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Neck assembly and test procedure. 572.163 Section... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.163 Neck assembly and test procedure. The neck assembly is assembled and tested as specified in 49 CFR 572.123 (Subpart N). ...

49 CFR 572.162 - Head assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Head assembly and test procedure. 572.162 Section... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.162 Head assembly and test procedure. The head assembly is assembled and tested as specified in 49 CFR 572.122 (Subpart N). ...

49 CFR 572.163 - Neck assembly and test procedure.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 7 2010-10-01 2010-10-01 false Neck assembly and test procedure. 572.163 Section... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.163 Neck assembly and test procedure. The neck assembly is assembled and tested as specified in 49 CFR 572.123 (Subpart N). ...

49 CFR 572.163 - Neck assembly and test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Neck assembly and test procedure. 572.163 Section... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.163 Neck assembly and test procedure. The neck assembly is assembled and tested as specified in 49 CFR 572.123 (Subpart N). ...

Assembly, alignment and test of the Transiting Exoplanet Survey Satellite (TESS) optical assemblies

NASA Astrophysics Data System (ADS)

Balonek, Gregory; Brown, Joshua J.; Andre, James E.; Chesbrough, Christian D.; Chrisp, Michael P.; Dalpiaz, Michael; Lennon, Joseph; Richards, B. C.; Clark, Kristin E.

2017-08-01

The Transiting Exoplanet Survey Satellite (TESS) will carry four visible waveband, seven-element, refractive F/1.4 lenses, each with a 34 degree diagonal field of view. This paper describes the methods used for the assembly, alignment and test of the four flight optical assemblies. Prior to commencing the build of the four flight optical assemblies, a Risk Reduction Unit (RRU) was successfully assembled and tested [1]. The lessons learned from the RRU were applied to the build of the flight assemblies. The main modifications to the flight assemblies include the inking of the third lens element stray light mitigation, tighter alignment tolerances, and diamond turning for critical mechanical surfaces. Each of the optical assemblies was tested interferometrically and measured with a low coherence distance measuring interferometer (DMI) to predict the optimal shim thickness between the lens assembly and detector before -75°C environmental testing. In addition to individual test data, environmental test results from prior assemblies allow for the exploration of marginal performance differences between each of the optical assemblies.

Multiple lead seal assembly for a liquid-metal-cooled fast-breeder nuclear reactor

DOEpatents

Hutter, Ernest; Pardini, John A.

1977-03-15

A reusable multiple lead seal assembly provides leak-free passage of stainless-steel-clad instrument leads through the cover on the primary tank of a liquid-metal-cooled fast-breeder nuclear reactor. The seal isolates radioactive argon cover gas and sodium vapor within the primary tank from the exterior atmosphere and permits reuse of the assembly and the stainless-steel-clad instrument leads. Leads are placed in flutes in a seal body, and a seal shell is then placed around the seal body. Circumferential channels in the body and inner surface of the shell are contiguous and together form a conduit which intersects each of the flutes, placing them in communication with a port through the wall of the seal shell. Liquid silicone rubber sealant is injected into the flutes through the port and conduit; the sealant fills the space in the flutes not occupied by the leads themselves and dries to a rubbery hardness. A nut, threaded onto a portion of the seal body not covered by the seal shell, jacks the body out of the shell and shears the sealant without damage to the body, shell, or leads. The leads may then be removed from the body. The sheared sealant is cleaned from the body, leads, and shell and the assembly may then be reused with the same or different leads.

Meshing complex macro-scale objects into self-assembling bricks

PubMed Central

Hacohen, Adar; Hanniel, Iddo; Nikulshin, Yasha; Wolfus, Shuki; Abu-Horowitz, Almogit; Bachelet, Ido

2015-01-01

Self-assembly provides an information-economical route to the fabrication of objects at virtually all scales. However, there is no known algorithm to program self-assembly in macro-scale, solid, complex 3D objects. Here such an algorithm is described, which is inspired by the molecular assembly of DNA, and based on bricks designed by tetrahedral meshing of arbitrary objects. Assembly rules are encoded by topographic cues imprinted on brick faces while attraction between bricks is provided by embedded magnets. The bricks can then be mixed in a container and agitated, leading to properly assembled objects at high yields and zero errors. The system and its assembly dynamics were characterized by video and audio analysis, enabling the precise time- and space-resolved characterization of its performance and accuracy. Improved designs inspired by our system could lead to successful implementation of self-assembly at the macro-scale, allowing rapid, on-demand fabrication of objects without the need for assembly lines. PMID:26226488

Magnesium Diboride Current Leads

NASA Technical Reports Server (NTRS)

Panek, John

2010-01-01

A recently discovered superconductor, magnesium diboride (MgB2), can be used to fabricate conducting leads used in cryogenic applications. Dis covered to be superconducting in 2001, MgB2 has the advantage of remaining superconducting at higher temperatures than the previously used material, NbTi. The purpose of these leads is to provide 2 A of electricity to motors located in a 1.3 K environment. The providing environment is a relatively warm 17 K. Requirements for these leads are to survive temperature fluctuations in the 5 K and 11 K heat sinks, and not conduct excessive heat into the 1.3 K environment. Test data showed that each lead in the assembly could conduct 5 A at 4 K, which, when scaled to 17 K, still provided more than the required 2 A. The lead assembly consists of 12 steelclad MgB2 wires, a tensioned Kevlar support, a thermal heat sink interface at 4 K, and base plates. The wires are soldered to heavy copper leads at the 17 K end, and to thin copper-clad NbTi leads at the 1.3 K end. The leads were designed, fabricated, and tested at the Forschungszentrum Karlsruhe - Institut foer Technische Physik before inclusion in Goddard's XRS (X-Ray Spectrometer) instrument onboard the Astro-E2 spacecraft. A key factor is that MgB2 remains superconducting up to 30 K, which means that it does not introduce joule heating as a resistive wire would. Because the required temperature ranges are 1.3-17 K, this provides a large margin of safety. Previous designs lost superconductivity at around 8 K. The disadvantage to MgB2 is that it is a brittle ceramic, and making thin wires from it is challenging. The solution was to encase the leads in thin steel tubes for strength. Previous designs were so brittle as to risk instrument survival. MgB2 leads can be used in any cryogenic application where small currents need to be conducted at below 30 K. Because previous designs would superconduct only at up to 8 K, this new design would be ideal for the 8-30 K range.

A Boundary Scan Test Vehicle for Direct Chip Attach Testing

NASA Technical Reports Server (NTRS)

Parsons, Heather A.; DAgostino, Saverio; Arakaki, Genji

2000-01-01

To facilitate the new faster, better and cheaper spacecraft designs, smaller more mass efficient avionics and instruments are using higher density electronic packaging technologies such as direct chip attach (DCA). For space flight applications, these technologies need to have demonstrated reliability and reasonably well defined fabrication and assembly processes before they will be accepted as baseline designs in new missions. As electronics shrink in size, not only can repair be more difficult, but 49 probing" circuitry can be very risky and it becomes increasingly more difficult to identify the specific source of a problem. To test and monitor these new technologies, the Direct Chip Attach Task, under NASA's Electronic Parts and Packaging Program (NEPP), chose the test methodology of boundary scan testing. The boundary scan methodology was developed for interconnect integrity and functional testing at hard to access electrical nodes. With boundary scan testing, active devices are used and failures can be identified to the specific device and lead. This technology permits the incorporation of "built in test" into almost any circuit and thus gives detailed test access to the highly integrated electronic assemblies. This presentation will describe boundary scan, discuss the development of the boundary scan test vehicle for DCA and current plans for testing of direct chip attach configurations.

Recommended design and fabrication sequence of AMTEC test assembly

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schock, A.; Kumar, V.; Noravian, H.

1998-01-01

A series of previous OSC papers described: 1) a novel methodology for the coupled thermal, fluid flow, and electrical analysis of multitube AMTEC (Alkali Metal Thermal-to-Electric Conversion) cells; 2) the application of that methodology to determine the effect of numerous design variations on the cell{close_quote}s performance, leading to selection and performance characterization of an OSC-recommended cell design; and 3) the design, analysis, and characterization of an OSC-generated power system design combining sixteen of the above AMTEC cells with two or three GPHS (General Purpose Heat Source) radioisotope heat source modules, and the applicability of those power systems to future spacemore » missions ({ital e.g.} Pluto Express and Europa Orbiter) under consideration by NASA. The OSC system design studies demonstrated the critical importance of the thermal insulation subsystem, and culminated in a design in which the eight AMTEC cells on each end of the heat source stack are embedded in Min-K fibrous insulation, and the Min-K and the GPHS modules are surrounded by graded-length Mo multifoil insulation. The present paper depicts the OSC-recommended AMTEC cell and generator designs, and identifies the need for an electrically heated (scaled-down but otherwise prototypic) test assembly for the experimental validation of the generator{close_quote}s system performance predictions. It then describes the design of an OSC-recommended test assembly consisting of an electrical heater enclosed in a graphite box to simulate the radioisotope heat source, four series-connected prototypic AMTEC cells of the OSC-recommended configuration, and a prototypic hybrid insulation package consisting of Min-K and graded-length Mo multifoils. Finally, the paper describes and illustrates an OSC-recommended detailed fabrication sequence and procedure for the above cell and test assembly. That fabrication procedure is being implemented by AMPS, Inc. with the support of DOE{close_quote}s Oak Ridge and Mound Laboratories, and the Air Force Phillips Laboratory (AFPL) will test the performance of the assembly over a range of input thermal powers and output voltages. The experimentally measured performance will be compared with the results of OSC analyses of the same insulated test assembly over the same range of operating parameters. {copyright} {ital 1998 American Institute of Physics.}« less

First environmental data from the EUV engineering test stand

NASA Astrophysics Data System (ADS)

Klebanoff, Leonard E.; Malinowski, Michael E.; Grunow, Philip A.; Clift, W. Miles; Steinhaus, Chip; Leung, Alvin H.; Haney, Steven J.

2001-08-01

The first environmental data from the Engineering Test Stand (ETS) has been collected. Excellent control of high-mass hydrocarbons has been observed. This control is a result of extensive outgas testing of components and materials, vacuum compatible design of the ETS, careful cleaning of parts and pre-baking of cables and sub assemblies where possible, and clean assembly procedures. As a result of the hydrocarbon control, the residual ETS vacuum environment is rich in water vapor. Analysis of witness plate data indicates that the ETS environment does not pose a contamination risk to the optics in the absence of EUV irradiation. However, with EUV exposure, the water rich environment can lead to EUV- induced water oxidation of the Si-terminated Mo/Si optics. Added ethanol can prevent optic oxidation, allowing carbon growth via EUV cracking of low-level residual hydrocarbons to occur. The EUV environmental issues are understood, mitigation approaches have been validated, and EUV optic contamination appears to be manageable.

CFRP composite optical telescope assembly for the 1 m ULTRA project

NASA Astrophysics Data System (ADS)

Martin, Robert N.; Romeo, Robert C.

2006-06-01

The focus of the ULTRA Project is to develop and test Ultra-Lightweight Technology for Research applications in Astronomy. The ULTRA project is a collaborative effort involving the private firm Composite Mirror Applications, Inc (CMA) and 3 universities: University of Kansas, San Diego State University, and Dartmouth College. Funding for ULTRA is predominately from a NSF three year MRI program grant to CMA and KU with additional support from CMA, KU and SDSU. The goal of the ULTRA program is to demonstrate that a viable alternative exists to traditional glass mirror and steel telescope technology by designing, fabricating and testing a research telescope constructed from carbon fiber reinforced plastic (CFRP) materials. In particular, a 1m diameter, Cassegrain telescope optics set and optical tube assembly (OTA) are being designed and fabricated by CMA. The completed telescope will be deployed at SDSU's Mt Laguna Observatory in a refurbished structure (new dome and mount provided via KU and SDSU). We expect that a successful completion and testing of this project will lead to future use of CFRP technology in larger telescopes and segmented telescopes. This paper describes the OTA (optical tube assembly) that has been developed for the ULTRA project. The mirror technology is described in another paper in this conference. A poster describes the ULTRA project overview in more detail.

Evaluating print performance of Sn-Ag-Cu lead-free solder pastes used in electronics assembly process

NASA Astrophysics Data System (ADS)

Mallik, S.; Bauer, R.; Hübner, F.; Ekere, N. N.

2011-01-01

Solder paste is the most widely used interconnection material in the electronic assembly process for attaching electronic components/devices directly onto the surface of printed circuit boards, using stencil printing process. This paper evaluates the performance of three different commercially available Sn-Ag-Cu solder pastes formulated with different particle size distributions (PSD), metal content and alloy composition. A series of stencil printing tests were carried out using a specially designed stencil of 75 μm thickness and apertures of 300×300 μm2 dimension and 500 μm pitch sizes. Solder paste printing behaviors were found related to attributes such as slumping and surface tension and printing performance was correlated with metal content and PSD. The results of the study should benefit paste manufacturers and SMT assemblers to improve their products and practices.

Computational design of a self-assembling symmetrical β-propeller protein.

PubMed

Voet, Arnout R D; Noguchi, Hiroki; Addy, Christine; Simoncini, David; Terada, Daiki; Unzai, Satoru; Park, Sam-Yong; Zhang, Kam Y J; Tame, Jeremy R H

2014-10-21

The modular structure of many protein families, such as β-propeller proteins, strongly implies that duplication played an important role in their evolution, leading to highly symmetrical intermediate forms. Previous attempts to create perfectly symmetrical propeller proteins have failed, however. We have therefore developed a new and rapid computational approach to design such proteins. As a test case, we have created a sixfold symmetrical β-propeller protein and experimentally validated the structure using X-ray crystallography. Each blade consists of 42 residues. Proteins carrying 2-10 identical blades were also expressed and purified. Two or three tandem blades assemble to recreate the highly stable sixfold symmetrical architecture, consistent with the duplication and fusion theory. The other proteins produce different monodisperse complexes, up to 42 blades (180 kDa) in size, which self-assemble according to simple symmetry rules. Our procedure is suitable for creating nano-building blocks from different protein templates of desired symmetry.

Solid state electro-optic color filter and iris

NASA Technical Reports Server (NTRS)

1974-01-01

The electro-optic properties of lanthanum-modified lead zirconate titanate (PLZT) ferroelectric ceramic material are evaluated when utilized as a variable density and/or spectral filter in conjunction with a television scanning system. Emphasis was placed on the development of techniques and procedures for processing the PLZT disks and for applying efficient electrode structures. A number of samples were processed using different combinations of cleaning, electrode material, and deposition process. Best overall performance resulted from the direct evaporation of gold over chrome electrodes. A ruggedized mounting holder assembly was designed, fabricated, and tested. The assembly provides electrical contacts, high voltage protection, and support for the fragile PLZT disk, and permits mounting and optical alignment of the associated polarizers. Operational measurements of a PLZT sample mounted in the holder assembly were performed in conjunction with a television camera and the associated drive circuits. The data verified achievement of the elimination of the observed white-line effect.

Development of an extended-burnup Mark B design. First semi-annual progress report, July-December 1978. Report BAW-1532-1. [PWR

DOE Office of Scientific and Technical Information (OSTI.GOV)

None

1979-10-01

The primary objective of this program is to develop and demonstrate an improved PWR fuel assembly design capable of batch average burnups of 45,000-50,000 MWd/mtU. To accomplish this, a number of technical areas must be investigated to verify acceptable extended-burnup fuel performance. This report is the first semi-annual progress report for the program, and it describes work performed during the July-December 1978 time period. Efforts during this period included the definition of a preliminary design for a high-burnup fuel rod, physics analyses of extended-burnup fuel cycles, studies of the physics characteristics of changes in fuel assembly metal-to-water ratios, and developmentmore » of a design concept for post-irradiation examination equipment to be utilized in examining high-burnup lead-test assemblies.« less

49 CFR 572.152 - Head assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Head assembly and test procedure. 572.152 Section... 12-Month-Old Infant, Alpha Version § 572.152 Head assembly and test procedure. (a) The head assembly (refer to § 572.150(a)(1)(i)) for this test consists of the assembly (drawing 921022-001), triaxial mount...

49 CFR 572.152 - Head assembly and test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Head assembly and test procedure. 572.152 Section... 12-Month-Old Infant, Alpha Version § 572.152 Head assembly and test procedure. (a) The head assembly (refer to § 572.150(a)(1)(i)) for this test consists of the assembly (drawing 921022-001), triaxial mount...

49 CFR 572.152 - Head assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Head assembly and test procedure. 572.152 Section... 12-Month-Old Infant, Alpha Version § 572.152 Head assembly and test procedure. (a) The head assembly (refer to § 572.150(a)(1)(i)) for this test consists of the assembly (drawing 921022-001), triaxial mount...

49 CFR 572.152 - Head assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Head assembly and test procedure. 572.152 Section... 12-Month-Old Infant, Alpha Version § 572.152 Head assembly and test procedure. (a) The head assembly (refer to § 572.150(a)(1)(i)) for this test consists of the assembly (drawing 921022-001), triaxial mount...

Feedbacks between community assembly and habitat selection shape variation in local colonization

USGS Publications Warehouse

Kraus, J.M.; Vonesh, J.R.

2010-01-01

1. Non-consumptive effects of predators are increasingly recognized as important drivers of community assembly and structure. Specifically, habitat selection responses to top predators during colonization and oviposition can lead to large differences in aquatic community structure, composition and diversity. 2. These differences among communities due to predators may develop as communities assemble, potentially altering the relative quality of predator vs. predator-free habitats through time. If so, community assembly would be expected to modify the subsequent behavioural responses of colonists to habitats containing top predators. Here, we test this hypothesis by manipulating community assembly and the presence of fish in experimental ponds and measuring their independent and combined effects on patterns of colonization by insects and amphibians. 3. Assembly modified habitat selection of dytscid beetles and hylid frogs by decreasing or even reversing avoidance of pools containing blue-spotted sunfish (Enneacanthus gloriosus). However, not all habitat selection responses to fish depended on assembly history. Hydrophilid beetles and mosquitoes avoided fish while chironomids were attracted to fish pools, regardless of assembly history. 4. Our results show that community assembly causes taxa-dependent feedbacks that can modify avoidance of habitats containing a top predator. Thus, non-consumptive effects of a top predator on community structure change as communities assemble and effects of competitors and other predators combine with the direct effects of top predators to shape colonization. 5. This work reinforces the importance of habitat selection for community assembly in aquatic systems, while illustrating the range of factors that may influence colonization rates and resulting community structure. Directly manipulating communities both during colonization and post-colonization is critical for elucidating how sequential processes interact to shape communities.

Development of a preprototype vapor compression distillation water recovery subsystem

NASA Technical Reports Server (NTRS)

Johnson, K. L.

1978-01-01

The activities involved in the design, development, and test of a preprototype vapor compression distillation water recovery subsystem are described. This subsystem, part of a larger regenerative life support evaluation system, is designed to recover usable water from urine, urinal rinse water, and concentrated shower and laundry brine collected from three space vehicle crewmen for a period of 180 days without resupply. Details of preliminary design and testing as well as component developments are included. Trade studies, considerations leading to concept selections, problems encountered, and test data are also presented. The rework of existing hardware, subsystem development including computer programs, assembly verification, and comprehensive baseline test results are discussed.

ITER Construction—Plant System Integration

NASA Astrophysics Data System (ADS)

Tada, E.; Matsuda, S.

2009-02-01

This brief paper introduces how the ITER will be built in the international collaboration. The ITER Organization plays a central role in constructing ITER and leading it into operation. Since most of the ITER components are to be provided in-kind from the member countries, integral project management should be scoped in advance of real work. Those include design, procurement, system assembly, testing, licensing and commissioning of ITER.

Gamma ray detector shield

DOEpatents

Ohlinger, R.D.; Humphrey, H.W.

1985-08-26

A gamma ray detector shield comprised of a rigid, lead, cylindrical-shaped vessel having upper and lower portions with an pneumatically driven, sliding top assembly. Disposed inside the lead shield is a gamma ray scintillation crystal detector. Access to the gamma detector is through the sliding top assembly.

49 CFR 572.122 - Head assembly and test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Head assembly and test procedure. 572.122 Section...-year-old Child Test Dummy, Beta Version § 572.122 Head assembly and test procedure. (a) The head assembly for this test consists of the complete head (drawing 127-1000), a six-axis neck transducer...

49 CFR 572.122 - Head assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Head assembly and test procedure. 572.122 Section...-year-old Child Test Dummy, Beta Version § 572.122 Head assembly and test procedure. (a) The head assembly for this test consists of the complete head (drawing 127-1000), a six-axis neck transducer...

49 CFR 572.122 - Head assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Head assembly and test procedure. 572.122 Section...-year-old Child Test Dummy, Beta Version § 572.122 Head assembly and test procedure. (a) The head assembly for this test consists of the complete head (drawing 127-1000), a six-axis neck transducer...

49 CFR 572.122 - Head assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Head assembly and test procedure. 572.122 Section...-year-old Child Test Dummy, Beta Version § 572.122 Head assembly and test procedure. (a) The head assembly for this test consists of the complete head (drawing 127-1000), a six-axis neck transducer...

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reiersen, W.; Heitzenroeder, P.; Neilson, G. H.

The National Compact Stellarator Experiment (NCSX) is being constructed at the Princeton Plasma Physics Laboratory (PPPL) in partnership with the Oak Ridge National Laboratory (ORNL). The stellarator core is designed to produce a compact 3-D plasma that combines stellarator and tokamak physics advantages. The engineering challenges of NCSX stem from its complex geometry. From the project's start in April, 2003 to September, 2004, the fabrication specifications for the project's two long-lead components, the modular coil winding forms and the vacuum vessel, were developed. An industrial manufacturing R&D program refined the processes for their fabrication as well as production cost andmore » schedule estimates. The project passed a series of reviews and established its performance baseline with the Department of Energy. In September 2004, fabrication was approved and contracts for these components were awarded. The suppliers have completed the engineering and tooling preparations and are in production. Meanwhile, the project completed preparations for winding the coils at PPPL by installing a coil manufacturing facility and developing all necessary processes through R&D. The main activities for the next two years will be component manufacture, coil winding, and sub-assembly of the vacuum vessel and coil subsets. Machine sector sub-assembly, machine assembly, and testing will follow, leading to First Plasma in July 2009.« less

Hi-rel lead-free printed wiring assemblies

NASA Technical Reports Server (NTRS)

Mehta, A. C.; Bonner, J. K.; Castillo, L. del

2002-01-01

The use of lead in electronics has come under increasing scrutiny. Given the trends in both Japan and Europe, it is highly likely that the U.S. will be driven by commercial interests to phase out of lead in electronics usage. This paper presents data collected on a recent NASA project to focus on finding suitable alternatives to eutectic tin-lead solders and solder pastes. The first phase of this project dealt with determining the most feasible candidates to replace tin-lead and to determine suitable processing operations in assemblies printed wiring boards.

Chronic exposure to particulate chromate induces spindle assembly checkpoint bypass in human lung cells.

PubMed

Wise, Sandra S; Holmes, Amie L; Xie, Hong; Thompson, W Douglas; Wise, John Pierce

2006-11-01

One of the hallmarks of lung cancer is chromosome instability (CIN), particularly a tetraploid phenotype, which is normally prevented by the spindle assembly checkpoint. Hexavalent chromium Cr(VI) is an established human lung carcinogen, and Cr(VI) induces tumors at lung bifurcation sites where Cr(VI) particles impact and persist. However, the effects of Cr(VI) on the spindle assembly checkpoint are unknown and little is known about prolonged exposure to particulate Cr(VI). Accordingly, we investigated particulate Cr(VI)-induced bypass of the spindle assembly checkpoint after several days of exposure in WHTBF-6 cells. We found that lead chromate indeed induces spindle assembly checkpoint bypass in human lung cells, as 72, 96, and 120 h treatments with 0.5 or 1 microg/cm2 lead chromate induced significant increases in the percentage of cells with aberrant mitotic figures. For example, treatment with 1 microg/cm2 lead chromate for 96 h induced 11, 12.3, and 14% of cells with premature anaphase, centromere spreading and premature centromere division, respectively. In addition, we found a disruption of mitosis with more cells accumulating in anaphase; cells treated for 96 h increased from 18% in controls to 31% in cells treated with lead chromate. To confirm involvement of the spindle assembly checkpoint, Mad2 expression was used as a marker. Mad2 expression was decreased in cells exposed to chronic treatments of lead chromate, consistent with disruption of the checkpoint. We also found concentration- and time-dependent increases in tetraploid cells, which continued to grow and form colonies. When cells were treated with chronic lead alone there was no increase in aberrant mitotic cells or polyploidy; however, chronic exposure to a soluble Cr(VI) showed an increase in aberrant mitotic cells and polyploidy. These data suggest that lead chromate does induce CIN and may be one mechanism in the development of Cr(VI)-induced lung cancer.

77 FR 35306 - Airworthiness Directives; Bell Helicopter Textron, Inc. Helicopters

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-13

... assemblies (power cable assemblies). This proposed AD is prompted by the determination that the power cable assembly connector (connector) can deteriorate, causing a short in the connector that may lead to a fire. This AD would require replacing the power cable assemblies and their associated parts, and performing...

49 CFR 572.172 - Head assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Head assembly and test procedure. 572.172 Section... Hybrid III 10-Year-Old Child Test Dummy (HIII-10C) § 572.172 Head assembly and test procedure. (a) The head assembly for this test consists of the complete head (drawing 420-1000), a six-axis neck...

49 CFR 572.142 - Head assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Head assembly and test procedure. 572.142 Section...-year-Old Child Crash Test Dummy, Alpha Version § 572.142 Head assembly and test procedure. (a) The head assembly (refer to § 572.140(a)(1)(i)) for this test consists of the head (drawing 210-1000), adapter plate...

49 CFR 572.172 - Head assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Head assembly and test procedure. 572.172 Section... Hybrid III 10-Year-Old Child Test Dummy (HIII-10C) § 572.172 Head assembly and test procedure. (a) The head assembly for this test consists of the complete head (drawing 420-1000), a six-axis neck...

49 CFR 572.172 - Head assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Head assembly and test procedure. 572.172 Section... Hybrid III 10-Year-Old Child Test Dummy (HIII-10C) § 572.172 Head assembly and test procedure. (a) The head assembly for this test consists of the complete head (drawing 420-1000), a six-axis neck...

49 CFR 572.132 - Head assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Head assembly and test procedure. 572.132 Section... Hybrid III 5th Percentile Female Test Dummy, Alpha Version § 572.132 Head assembly and test procedure. (a) The head assembly (refer to § 572.130(a)(1)(i)) for this test consists of the complete head (drawing...

49 CFR 572.142 - Head assembly and test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Head assembly and test procedure. 572.142 Section...-year-Old Child Crash Test Dummy, Alpha Version § 572.142 Head assembly and test procedure. (a) The head assembly (refer to § 572.140(a)(1)(i)) for this test consists of the head (drawing 210-1000), adapter plate...

49 CFR 572.132 - Head assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Head assembly and test procedure. 572.132 Section... Hybrid III 5th Percentile Female Test Dummy, Alpha Version § 572.132 Head assembly and test procedure. (a) The head assembly (refer to § 572.130(a)(1)(i)) for this test consists of the complete head (drawing...

49 CFR 572.142 - Head assembly and test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Head assembly and test procedure. 572.142 Section...-year-Old Child Crash Test Dummy, Alpha Version § 572.142 Head assembly and test procedure. (a) The head assembly (refer to § 572.140(a)(1)(i)) for this test consists of the head (drawing 210-1000), adapter plate...

49 CFR 572.132 - Head assembly and test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Head assembly and test procedure. 572.132 Section... Hybrid III 5th Percentile Female Test Dummy, Alpha Version § 572.132 Head assembly and test procedure. (a) The head assembly (refer to § 572.130(a)(1)(i)) for this test consists of the complete head (drawing...

49 CFR 572.132 - Head assembly and test procedure.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 7 2010-10-01 2010-10-01 false Head assembly and test procedure. 572.132 Section... Hybrid III 5th Percentile Female Test Dummy, Alpha Version § 572.132 Head assembly and test procedure. (a) The head assembly (refer to § 572.130(a)(1)(i)) for this test consists of the complete head (drawing...

49 CFR 572.142 - Head assembly and test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Head assembly and test procedure. 572.142 Section...-year-Old Child Crash Test Dummy, Alpha Version § 572.142 Head assembly and test procedure. (a) The head assembly (refer to § 572.140(a)(1)(i)) for this test consists of the head (drawing 210-1000), adapter plate...

49 CFR 572.132 - Head assembly and test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Head assembly and test procedure. 572.132 Section... Hybrid III 5th Percentile Female Test Dummy, Alpha Version § 572.132 Head assembly and test procedure. (a) The head assembly (refer to § 572.130(a)(1)(i)) for this test consists of the complete head (drawing...

49 CFR 572.135 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Upper and lower torso assemblies and torso flexion... torso assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is... (drawing 880105-434), on resistance to articulation between the upper torso assembly (drawing 880105-300...

49 CFR 572.135 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Upper and lower torso assemblies and torso flexion... torso assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is... (drawing 880105-434), on resistance to articulation between the upper torso assembly (drawing 880105-300...

49 CFR 572.125 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is to...), and abdominal insert (drawing 127-8210), on resistance to articulation between upper torso assembly...

49 CFR 572.135 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Upper and lower torso assemblies and torso flexion... torso assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is... (drawing 880105-434), on resistance to articulation between the upper torso assembly (drawing 880105-300...

49 CFR 572.125 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is to...), and abdominal insert (drawing 127-8210), on resistance to articulation between upper torso assembly...

49 CFR 572.125 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is to...), and abdominal insert (drawing 127-8210), on resistance to articulation between upper torso assembly...

49 CFR 572.135 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Upper and lower torso assemblies and torso flexion... torso assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is... (drawing 880105-434), on resistance to articulation between the upper torso assembly (drawing 880105-300...

49 CFR 572.125 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is to...), and abdominal insert (drawing 127-8210), on resistance to articulation between upper torso assembly...

Self-assembly of pi-conjugated peptides in aqueous environments leading to energy-transporting bioelectronic nanostructures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tavor, John

The realization of new supramolecular pi-conjugated organic structures inspired and driven by peptide-based self-assembly will offer a new approach to interface with the biotic environment in a way that will help to meet many DOE-recognized grand challenges. Previously, we developed pi-conjugated peptides that undergo supramolecular self-assembly into one-dimensional (1-D) organic electronic nanomaterials under benign aqueous conditions. The intermolecular interactions among the pi-conjugated organic segments within these nanomaterials lead to defined perturbations of their optoelectronic properties and yield nanoscale conduits that support energy transport within individual nanostructures and throughout bulk macroscopic collections of nanomaterials. Our objectives for future research are tomore » construct and study biomimetic electronic materials for energy-related technology optimized for harsher non-biological environments where peptide-driven self-assembly enhances pi-stacking within nanostructured biomaterials, as detailed in the following specific tasks: (1) synthesis and detailed optoelectronic characterization of new pi-electron units to embed within homogeneous self assembling peptides, (2) molecular and data-driven modeling of the nanomaterial aggregates and their higher-order assemblies, and (3) development of new hierarchical assembly paradigms to organize multiple electronic subunits within the nanomaterials leading to heterogeneous electronic properties (i.e. gradients and localized electric fields). These intertwined research tasks will lead to the continued development and fundamental mechanistic understanding of a powerful bioinspired materials set capable of making connections between nanoscale electronic materials and macroscopic bulk interfaces, be they those of a cell, a protein or a device.« less

Neutronics, steady-state, and transient analyses for the Poland MARIA reactor for irradiation testing of LEU lead test fuel assemblies from CERCA : ANL independent verification results.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Garner, P. L.; Hanan, N. A.

The MARIA reactor at the Institute of Atomic Energy (IAE) in Swierk (30 km SE of Warsaw) in the Republic of Poland is considering conversion from high-enriched uranium (HEU) to low-enriched uranium (LEU) fuel assemblies (FA). The FA design in MARIA is rather unique; a suitable LEU FA has never been designed or tested. IAE has contracted with CERCA (the fuel supply portion of AREVA in France) to supply 2 lead test assemblies (LTA). The LTAs will be irradiated in MARIA to burnup level of at least 40% for both LTAs and to 60% for one LTA. IAE may decidemore » to purchase additional LEU FAs for a full core conversion after the test irradiation. The Reactor Safety Committee within IAE and the National Atomic Energy Agency in Poland (PAA) must approve the LTA irradiation process. The approval will be based, in part, on IAE submitting revisions to portions of the Safety Analysis Report (SAR) which are affected by the insertion of the LTAs. (A similar process will be required for the full core conversion to LEU fuel.) The analysis required was established during working meetings between Argonne National Laboratory (ANL) and IAE staff during August 2006, subsequent email correspondence, and subsequent staff visits. The analysis needs to consider the current high-enriched uranium (HEU) core and 4 core configurations containing 1 and 2 LEU LTAs in various core positions. Calculations have been performed at ANL in support of the LTA irradiation. These calculations are summarized in this report and include criticality, burn-up, neutronics parameters, steady-state thermal hydraulics, and postulated transients. These calculations have been performed at the request of the IAE staff, who are performing similar calculations to be used in their SAR amendment submittal to the PAA. The ANL analysis has been performed independently from that being performed by IAE and should only be used as one step in the verification process.« less

Bipolar battery construction

NASA Technical Reports Server (NTRS)

Edwards, Dean B. (Inventor); Rippel, Wally E. (Inventor)

1981-01-01

A lightweight, bipolar battery construction for lead acid batteries in which a plurality of thin, rigid, biplates each comprise a graphite fiber thermoplastic composition in conductive relation to lead stripes plated on opposite flat surfaces of the plates, and wherein a plurality of nonconductive thermoplastic separator plates support resilient yieldable porous glass mats in which active material is carried, the biplates and separator plates with active material being contained and maintained in stacked assembly by axial compression of the stacked assembly. A method of assembling such a bipolar battery construction.

Update on Risk Reduction Activities for a Liquid Advanced Booster for NASA's Space Launch System

NASA Technical Reports Server (NTRS)

Crocker, Andrew M.; Greene, William D.

2017-01-01

The stated goals of NASA's Research Announcement for the Space Launch System (SLS) Advanced Booster Engineering Demonstration and/or Risk Reduction (ABEDRR) are to reduce risks leading to an affordable Advanced Booster that meets the evolved capabilities of SLS and enable competition by mitigating targeted Advanced Booster risks to enhance SLS affordability. Dynetics, Inc. and Aerojet Rocketdyne (AR) formed a team to offer a wide-ranging set of risk reduction activities and full-scale, system-level demonstrations that support NASA's ABEDRR goals. During the ABEDRR effort, the Dynetics Team has modified flight-proven Apollo-Saturn F-1 engine components and subsystems to improve affordability and reliability (e.g., reduce parts counts, touch labor, or use lower cost manufacturing processes and materials). The team has built hardware to validate production costs and completed tests to demonstrate it can meet performance requirements. State-of-the-art manufacturing and processing techniques have been applied to the heritage F-1, resulting in a low recurring cost engine while retaining the benefits of Apollo-era experience. NASA test facilities have been used to perform low-cost risk-reduction engine testing. In early 2014, NASA and the Dynetics Team agreed to move additional large liquid oxygen/kerosene engine work under Dynetics' ABEDRR contract. Also led by AR, the objectives of this work are to demonstrate combustion stability and measure performance of a 500,000 lbf class Oxidizer-Rich Staged Combustion (ORSC) cycle main injector. A trade study was completed to investigate the feasibility, cost effectiveness, and technical maturity of a domestically-produced engine that could potentially both replace the RD-180 on Atlas V and satisfy NASA SLS payload-to-orbit requirements via an advanced booster application. Engine physical dimensions and performance parameters resulting from this study provide the system level requirements for the ORSC risk reduction test article. The test article is scheduled to complete fabrication and assembly soon and continue testing through late 2019. Dynetics has also designed, developed, and built innovative tank and structure assemblies using friction stir welding to leverage recent NASA investments in manufacturing tools, facilities, and processes, significantly reducing development and recurring costs. The full-scale cryotank assembly was used to verify the structural design and prove affordable processes. Dynetics performed hydrostatic and cryothermal proof tests on the assembly to verify the assembly meets performance requirements..

49 CFR 572.165 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is to... (specified in 49 CFR 572.125(a)), on resistance to articulation between the upper torso assembly (drawing 167...

49 CFR 572.165 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is to... (specified in 49 CFR 572.125(a)), on resistance to articulation between the upper torso assembly (drawing 167...

49 CFR 572.165 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 7 2010-10-01 2010-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is to... (specified in 49 CFR 572.125(a)), on resistance to articulation between the upper torso assembly (drawing 167...

49 CFR 572.165 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is to... (specified in 49 CFR 572.125(a)), on resistance to articulation between the upper torso assembly (drawing 167...

49 CFR 572.165 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) Upper/lower torso assembly. The test objective is to... (specified in 49 CFR 572.125(a)), on resistance to articulation between the upper torso assembly (drawing 167...

Test-Analysis Correlation for Space Shuttle External Tank Foam Impacting RCC Wing Leading Edge Component Panels

NASA Technical Reports Server (NTRS)

Lyle, Karen H.

2008-01-01

The Space Shuttle Columbia Accident Investigation Board recommended that NASA develop, validate, and maintain a modeling tool capable of predicting the damage threshold for debris impacts on the Space Shuttle Reinforced Carbon-Carbon (RCC) wing leading edge and nosecap assembly. The results presented in this paper are one part of a multi-level approach that supported the development of the predictive tool used to recertify the shuttle for flight following the Columbia Accident. The assessment of predictive capability was largely based on test analysis comparisons for simpler component structures. This paper provides comparisons of finite element simulations with test data for external tank foam debris impacts onto 6-in. square RCC flat panels. Both quantitative displacement and qualitative damage assessment correlations are provided. The comparisons show good agreement and provided the Space Shuttle Program with confidence in the predictive tool.

Testing the equipment for the cryogenic optical test of the James Webb Space Telescope

NASA Astrophysics Data System (ADS)

Whitman, Tony L.; Dziak, K. J.; Huguet, Jesse; Knight, J. Scott; Reis, Carl; Wilson, Erin

2014-08-01

After integration of the Optical Telescope Element (OTE) to the Integrated Science Instrument Module (ISIM) to become the OTIS, the JWST optics are tested at NASA's Johnson Space Center (JSC) in the cryogenic vacuum Chamber A for alignment and optical performance. Tens of trucks full of custom test equipment are being delivered to the JSC, in addition to the large pieces built at the Center, and the renovation of the chamber itself. The facility is tested for the thermal stability control for optical measurements and contamination control during temperature transitions. The support for the OTIS is also tested for thermal stability control, load tested in the cryogenic environment, and tested for isolation of the background vibration for the optical measurements. The Center of Curvature Optical Assembly (COCOA) is tested for the phasing and wavefront error (WFE) measurement of an 18 segment mirror and for cryogenic operation. A photogrammetry system is tested for metrology performance and cryogenic operation. Test mirrors for auto-collimation measurements are tested for optical performance and cryogenic operation. An assembly of optical test sources are calibrated and tested in a cryogenic environment. A Pathfinder telescope is used as a surrogate telescope for cryogenic testing of the OTIS optical test configuration. A Beam Image Analyzer (BIA) is used as a surrogate ISIM with the Pathfinder in this test. After briefly describing the OTIS optical test configuration, the paper will overview the list and configuration of significant tests of the equipment leading up to the OTIS test.

Ergonomic risk assessment with DesignCheck to evaluate assembly work in different phases of the vehicle development process.

PubMed

Winter, Gabriele; Schaub, Karlheinz G; Großmann, Kay; Laun, Gerhard; Landau, Kurt; Bruder, Ralph

2012-01-01

Occupational hazards exist, if the design of the work situation is not in accordance with ergonomic design principles. At assembly lines ergonomics is applied to the design of work equipment and tasks and to work organisation. The ignoring of ergonomic principles in planning and design of assembly work leads to unfavourable working posture, action force and material handling. Disorders of the musculoskeletal system are of a common occurrence throughout Europe. Musculoskeletal disorders are a challenge against the background of disabled workers. The changes in a worker's capability have to be regarded in the conception of redesigned and new assembly lines. In this way ergonomics becomes progressively more important in planning and design of vehicles: The objective of ergonomic design in different stages of the vehicles development process is to achieve an optimal adaptation of the assembly work to workers. Hence the ergonomic screening tool "Design Check" (DC) was developed to identify ergonomic deficits in workplace layouts. The screening-tool is based on the current ergonomic state of the art in the design of physical work and relevant EU legal requirements. It was tested within a federal German research project at selected work stations at the assembly lines at Dr.-Ing. h.c. F. Porsche AG / Stuttgart. Meanwhile the application of the screening-tool DC is transferred in other parts of the Porsche AG, Stuttgart. It is also realized as an ergonomic standard method to perform assembly work in different phases of the vehicle development process.

Self Assembled Particles

NASA Technical Reports Server (NTRS)

Palacci, Jeremie (Inventor); Pine, David J. (Inventor); Chaikin, Paul Michael (Inventor); Sacanna, Stefano (Inventor)

2017-01-01

A self-assembling structure using non-equilibrium driving forces leading to 'living crystals' and other maniputable particles with a complex dynamics. The dynamic self-assembly assembly results from a competition between self-propulsion of particles and an attractive interaction between the particles. As a result of non-equilibrium driving forces, the crystals form, grow, collide, anneal, repair themselves and spontaneously self-destruct, thereby enabling reconfiguration and assembly to achieve a desired property.

A low redox potential affects monoclonal antibody assembly and glycosylation in cell culture.

PubMed

Dionne, Benjamin; Mishra, Neha; Butler, Michael

2017-03-20

Glycosylation and intracellular assembly of monoclonal antibodies (MAbs) is important for glycan profile consistency. To better understand how these factors may be influenced by a lower redox potential, an IgG1-producing NS0 cell line was grown in the presence of varying concentrations of dithiothreitol (DTT). Cultures were monitored for growth and culture redox potential (CRP) with glycan heterogeneity determined using a HILIC-HPLC method. Macroheterogeneity was unchanged in all conditions whereas the Galactosylation Index (GI) decreased by as much as 50% in cultures with lower CRP or higher dithiothreitol levels. This shift in GI is reflected in more agalactosylated and asialylated species being produced. The MAb assembly pathway was determined using radioactive isotope 35 S incorporated into nascent IgG1 molecules. The assembly pathway for this IgG1 was shown to progress via HC→HC 2 →HC 2 LC→HC 2 LC 2 in all conditions tested and autoradiographs highlighted that the ratio of heavy chain dimer to heavy chain monomer increased over time with increasing DTT concentrations. This increase and correspondingly lower GI values may be due to disruption of the disulfide bonds at higher levels of assembly. A change in the assembly pathway may alter the final IgG glycan pattern and lead to control mechanisms that influence glycan profiles of MAbs. Copyright © 2017. Published by Elsevier B.V.

Azaphilones inhibit tau aggregation and dissolve tau aggregates in vitro.

PubMed

Paranjape, Smita R; Riley, Andrew P; Somoza, Amber D; Oakley, C Elizabeth; Wang, Clay C C; Prisinzano, Thomas E; Oakley, Berl R; Gamblin, T Chris

2015-05-20

The aggregation of the microtubule-associated protein tau is a seminal event in many neurodegenerative diseases, including Alzheimer's disease. The inhibition or reversal of tau aggregation is therefore a potential therapeutic strategy for these diseases. Fungal natural products have proven to be a rich source of useful compounds having wide varieties of biological activities. We have previously screened Aspergillus nidulans secondary metabolites for their ability to inhibit tau aggregation in vitro using an arachidonic acid polymerization protocol. One aggregation inhibitor identified was asperbenzaldehyde, an intermediate in azaphilone biosynthesis. We therefore tested 11 azaphilone derivatives to determine their tau assembly inhibition properties in vitro. All compounds tested inhibited tau filament assembly to some extent, and four of the 11 compounds had the advantageous property of disassembling preformed tau aggregates in a dose-dependent fashion. The addition of these compounds to the tau aggregates reduced both the total length and number of tau polymers. The most potent compounds were tested in in vitro reactions to determine whether they interfere with tau's normal function of stabilizing microtubules (MTs). We found that they did not completely inhibit MT assembly in the presence of tau. These derivatives are very promising lead compounds for tau aggregation inhibitors and, more excitingly, for compounds that can disassemble pre-existing tau filaments. They also represent a new class of anti-tau aggregation compounds with a novel structural scaffold.

Tests of an alternate mobile transporter and extravehicular activity assembly procedure for the Space Station Freedom truss

NASA Technical Reports Server (NTRS)

Heard, Walter L., Jr.; Watson, Judith J.; Lake, Mark S.; Bush, Harold G.; Jensen, J. Kermit; Wallsom, Richard E.; Phelps, James E.

1992-01-01

Results are presented from a ground test program of an alternate mobile transporter (MT) concept and extravehicular activity (EVA) assembly procedure for the Space Station Freedom (SSF) truss keel. A three-bay orthogonal tetrahedral truss beam consisting of 44 2-in-diameter struts and 16 nodes was assembled repeatedly in neutral buoyancy by pairs of pressure-suited test subjects working from astronaut positioning devices (APD's) on the MT. The truss bays were cubic with edges 15 ft long. All the truss joint hardware was found to be EVA compatible. The average unit assembly time for a single pair of experienced test subjects was 27.6 sec/strut, which is about half the time derived from other SSF truss assembly tests. A concept for integration of utility trays during truss assembly is introduced and demonstrated in the assembly tests. The concept, which requires minimal EVA handling of the trays, is shown to have little impact on overall assembly time. The results of these tests indicate that by using an MT equipped with APD's, rapid EVA assembly of a space station-size truss structure can be expected.

TAB interconnects for space concentrator solar cell arrays

NASA Technical Reports Server (NTRS)

Avery, J.; Bauman, J. S.; Gallagher, P.; Yerkes, J. W.

1993-01-01

The Boeing Company has evaluated the use of Tape Automated Bonding (TAB) and Surface Mount Technology (SMT) for a highly reliable, low cost interconnect for concentrator solar cell arrays. TAB and SMT are currently used in the electronics industry for chip interconnects and printed circuit board assembly. TAB tape consists of sixty-four 3-mil/1-oz tin-plated copper leads on 8-mil centers. The leads are thermocompression gang bonded to GaAs concentrator solar cell with silver contacts. This bond, known as an Inner Lead Bond (ILB), allows for pretesting and sorting capability via nondestruct wire bond pull and flash testing. Destructive wire pull tests resulted in preferred mid-span failures. Improvements in fill factor were attributed to decreased contact resistance on TAB bonded cells. Preliminary thermal cycling and aging tests were shown excellent bond strength and metallurgical results. Auger scans of bond sites reveals an Ag-Cu-Tin composition. Improper bonds are identified through flash testing as a performance degradation. On going testing of cells are underway at Lewis Research Center. SMT techniques are utilized to excise and form TAB leads post ILB. The formed leads' shape isolates thermal mismatches between the cells and the flex circuit they are mounted on. TABed cells are picked and placed with a gantry x-y-z positioning system with pattern recognition. Adhesives are selected to avoid thermal expansion mismatch and promote thermal transfer to the flex circuit. TAB outer lead bonds are parallel gap welded (PGW) to the flex circuit to finish the concentrator solar cell subassembly.

Vibration Monitoring Using Fiber Optic Sensors in a Lead-Bismuth Eutectic Cooled Nuclear Fuel Assembly †

PubMed Central

De Pauw, Ben; Lamberti, Alfredo; Ertveldt, Julien; Rezayat, Ali; van Tichelen, Katrien; Vanlanduit, Steve; Berghmans, Francis

2016-01-01

Excessive fuel assembly vibrations in nuclear reactor cores should be avoided in order not to compromise the lifetime of the assembly and in order to prevent the occurrence of safety hazards. This issue is particularly relevant to new reactor designs that use liquid metal coolants, such as, for example, a molten lead-bismuth eutectic. The flow of molten heavy metal around and through the fuel assembly may cause the latter to vibrate and hence suffer degradation as a result of, for example, fretting wear or mechanical fatigue. In this paper, we demonstrate the use of optical fiber sensors to measure the fuel assembly vibration in a lead-bismuth eutectic cooled installation which can be used as input to assess vibration-related safety hazards. We show that the vibration characteristics of the fuel pins in the fuel assembly can be experimentally determined with minimal intrusiveness and with high precision owing to the small dimensions and properties of the sensors. In particular, we were able to record local strain level differences of about 0.2 μϵ allowing us to reliably estimate the vibration amplitudes and modal parameters of the fuel assembly based on optical fiber sensor readings during different stages of the operation of the facility, including the onset of the coolant circulation and steady-state operation. PMID:27110782

Assessment of Head Displacement and Disassembly Force With Increasing Assembly Load at the Head/Trunnion Junction of a Total Hip Arthroplasty Prosthesis.

PubMed

Ramoutar, Darryl N; Crosnier, Emilie A; Shivji, Faiz; Miles, Anthony W; Gill, Harinderjit S

2017-05-01

Most femoral components used now for total hip arthroplasty are modular, requiring a strong connection at assembly. The aim of this study was to assess the effect of assembly force on the strength of head-trunnion interface and to measure the initial displacement of the head on the trunnion with different assembly forces. Three assembly load levels were assessed (A: 2 kN, B: 4 kN, C: 6 kN) with 4 implants in each group. The stems were mounted in a custom rig and the respective assembly loads were applied to the head at a constant rate of 0.05 kN/s (ISO7260-10:2003). Load levels were recorded during assembly. Head displacement was measured with a laser sensor. The disassembly force was determined by a standard pull-off test. The maximum head displacement on the trunnion was significantly different between the 2 kN group and the other 2 groups (4 kN, 6 kN, P = .029), but not between the 4 kN and 6 kN groups (P = .89). The disassembly forces between the 3 groups were significantly different (mean ± standard deviation, A: 1316 ± 223 kN; B: 2224 ± 151 kN; C: 3965 ± 344 kN; P = .007), with increasing assembly load leading to a higher pull-off force. For the 4 kN and 6 kN groups, a first peak of approximately 2.5 kN was observed on the load recordings during assembly before the required assembly load was eventually reached corresponding to sudden increase in head displacement to approximately 150 μm. An assembly force of 2 kN may be too low to overcome the frictional forces needed to engage the head and achieve maximum displacement on the trunnion and thus an assembly load of greater than 2.5 kN is recommended. Copyright © 2016 Elsevier Inc. All rights reserved.

An Interactive Method to Solve Infeasibility in Linear Programming Test Assembling Models

ERIC Educational Resources Information Center

Huitzing, Hiddo A.

2004-01-01

In optimal assembly of tests from item banks, linear programming (LP) models have proved to be very useful. Assembly by hand has become nearly impossible, but these LP techniques are able to find the best solutions, given the demands and needs of the test to be assembled and the specifics of the item bank from which it is assembled. However,…

Genome assembly reborn: recent computational challenges

PubMed Central

2009-01-01

Research into genome assembly algorithms has experienced a resurgence due to new challenges created by the development of next generation sequencing technologies. Several genome assemblers have been published in recent years specifically targeted at the new sequence data; however, the ever-changing technological landscape leads to the need for continued research. In addition, the low cost of next generation sequencing data has led to an increased use of sequencing in new settings. For example, the new field of metagenomics relies on large-scale sequencing of entire microbial communities instead of isolate genomes, leading to new computational challenges. In this article, we outline the major algorithmic approaches for genome assembly and describe recent developments in this domain. PMID:19482960

Atomic Oxygen Exposure of Polyimide Foam for International Space Station Solar Array Wing Blanket Box

NASA Technical Reports Server (NTRS)

Finckenor, M. M.; Albyn, K. C.; Watts, E. W.

2006-01-01

Onorbit photos of the International Space Station (ISS) solar array blanket box foam pad assembly indicate degradation of the Kapton film covering the foam, leading to atomic oxygen (AO) exposure of the foam. The purpose of this test was to determine the magnitude of particulate generation caused by low-Earth orbital environment exposure of the foam and also by compression of the foam during solar array wing retraction. The polyimide foam used in the ISS solar array wing blanket box assembly is susceptible to significant AO erosion. The foam sample in this test lost one-third of its mass after exposure to the equivalent of 22 mo onorbit. Some particulate was generated by exposure to simulated orbital conditions and the simulated solar array retraction (compression test). However, onorbit, these particles would also be eroded by AO. The captured particles were generally <1 mm, and the particles shaken free of the sample had a maximum size of 4 mm. The foam sample maintained integrity after a compression load of 2.5 psi.

Reliability of high I/O high density CCGA interconnect electronic packages under extreme thermal environments

NASA Astrophysics Data System (ADS)

Ramesham, Rajeshuni

2012-03-01

Ceramic column grid array (CCGA) packages have been increasing in use based on their advantages such as high interconnect density, very good thermal and electrical performances, compatibility with standard surfacemount packaging assembly processes, and so on. CCGA packages are used in space applications such as in logic and microprocessor functions, telecommunications, payload electronics, and flight avionics. As these packages tend to have less solder joint strain relief than leaded packages or more strain relief over lead-less chip carrier packages, the reliability of CCGA packages is very important for short-term and long-term deep space missions. We have employed high density CCGA 1152 and 1272 daisy chained electronic packages in this preliminary reliability study. Each package is divided into several daisy-chained sections. The physical dimensions of CCGA1152 package is 35 mm x 35 mm with a 34 x 34 array of columns with a 1 mm pitch. The dimension of the CCGA1272 package is 37.5 mm x 37.5 mm with a 36 x 36 array with a 1 mm pitch. The columns are made up of 80% Pb/20%Sn material. CCGA interconnect electronic package printed wiring polyimide boards have been assembled and inspected using non-destructive x-ray imaging techniques. The assembled CCGA boards were subjected to extreme temperature thermal atmospheric cycling to assess their reliability for future deep space missions. The resistance of daisy-chained interconnect sections were monitored continuously during thermal cycling. This paper provides the experimental test results of advanced CCGA packages tested in extreme temperature thermal environments. Standard optical inspection and x-ray non-destructive inspection tools were used to assess the reliability of high density CCGA packages for deep space extreme temperature missions.

Phylogenetic relatedness and leaf functional traits, not introduced status, influence community assembly.

PubMed

Lemoine, Nathan P; Shue, Jessica; Verrico, Brittany; Erickson, David; Kress, W John; Parker, John D

2015-10-01

Considerable debate focuses on whether invasive species establish and become abundant by being functionally and phylogenetically distinct from native species, leading to a host of invasion-specific hypotheses of community assembly. Few studies, however, have quantitatively assessed whether similar patterns of phylogenetic and functional similarity explain local abundance of both native and introduced species, which would suggest similar assembly mechanisms regardless of origin. Using a chronosequence of invaded temperate forest stands, we tested whether the occurrence and abundance of both introduced and native species were predicted by phylogenetic relatedness, functional overlap, and key environmental characteristics including forest age. Environmental filtering against functionally and phylogenetically distinct species strongly dictated the occurrence and abundance of both introduced and native species, with slight modifications of these patterns according to forest age. Thus, once functional and evolutionary novelty were quantified, introduced status provided little information about species' presence or abundance, indicating largely similar sorting mechanisms for both native and introduced species.

Sol-Gel Electrolytes Incorporated by Lanthanide Luminescent Materials and Their Photophysical Properties

NASA Astrophysics Data System (ADS)

Yu, Chufang; Zhang, Zhengyang; Fu, Meizhen; Gao, Jinwei; Zheng, Yuhui

2017-10-01

A group of silica gel electrolytes with lanthanide luminescent hybrid materials were assembled and investigated. Photophysical studies showed that terbium and europium hybrids displayed characteristic green and red emissions within the electrolytes. The influence of different concentration of the lanthanide hybrids on the electrochemical behavior of a gelled electrolyte valve-regulated lead-acid battery were studied through cyclic voltammograms, electrochemical impedance spectroscopy, water holding experiments and mobility tests. The morphology and particle size were analyzed by scanning electron microscopy. The results proved that lanthanide (Tb3+/Eu3+) luminescent materials are effective additives which will significantly improve the electrochemical properties of lead-acid batteries.

Al/Pb lightweight grids prepared by molten salt electroless plating for application in lead-acid batteries

NASA Astrophysics Data System (ADS)

Hong, Bo; Jiang, Liangxing; Hao, Ketao; Liu, Fangyang; Yu, Xiaoying; Xue, Haitao; Li, Jie; Liu, Yexiang

2014-06-01

In this paper, a lightweight Pb plated Al (Al/Pb) grid was prepared by molten salt electroless plating. The SEM and bonding strength test show that the lead coating is deposited with a smooth surface and firm combination. CV test shows that the electrochemical properties of Al/Pb electrodes are stable. 2.0 V single-cell flooded lead-acid batteries with Al/Pb grids as negative collectors are assembled and the performances including 20 h capacity, rate capacity, cycle life, internal resistance are investigated. The results show that the cycle life of Al/Pb-grid cells is about 475 cycles and can meet the requirement of lead-acid batteries. Al/Pb grids are conducive to the refinement of PbSO4 grain, and thereby reduce the internal resistance of battery and advance the utilization of active mass. Moreover, weight of Al/Pb grid is only 55.4% of the conventional-grid. In this way, mass specific capacity of Al/Pb-grid negatives is 17.8% higher and the utilization of active mass is 6.5% higher than conventional-grid negatives.

Root Cause Investigation of Lead-Free Solder Joint Interfacial Failures After Multiple Reflows

NASA Astrophysics Data System (ADS)

Li, Yan; Hatch, Olen; Liu, Pilin; Goyal, Deepak

2017-03-01

Solder joint interconnects in three-dimensional (3D) packages with package stacking configurations typically must undergo multiple reflow cycles during the assembly process. In this work, interfacial open joint failures between the bulk solder and the intermetallic compound (IMC) layer were found in Sn-Ag-Cu (SAC) solder joints connecting a small package to a large package after multiple reflow reliability tests. Systematic progressive 3D x-ray computed tomography experiments were performed on both incoming and assembled parts to reveal the initiation and evolution of the open failures in the same solder joints before and after the reliability tests. Characterization studies, including focused ion beam cross-sections, scanning electron microscopy, and energy-dispersive x-ray spectroscopy, were conducted to determine the correlation between IMC phase transformation and failure initiation in the solder joints. A comprehensive failure mechanism, along with solution paths for the solder joint interfacial failures after multiple reflow cycles, is discussed in detail.

GRAbB: Selective Assembly of Genomic Regions, a New Niche for Genomic Research

PubMed Central

Zhang, Hao; van Diepeningen, Anne D.; van der Lee, Theo A. J.; Waalwijk, Cees; de Hoog, G. Sybren

2016-01-01

GRAbB (Genomic Region Assembly by Baiting) is a new program that is dedicated to assemble specific genomic regions from NGS data. This approach is especially useful when dealing with multi copy regions, such as mitochondrial genome and the rDNA repeat region, parts of the genome that are often neglected or poorly assembled, although they contain interesting information from phylogenetic or epidemiologic perspectives, but also single copy regions can be assembled. The program is capable of targeting multiple regions within a single run. Furthermore, GRAbB can be used to extract specific loci from NGS data, based on homology, like sequences that are used for barcoding. To make the assembly specific, a known part of the region, such as the sequence of a PCR amplicon or a homologous sequence from a related species must be specified. By assembling only the region of interest, the assembly process is computationally much less demanding and may lead to assemblies of better quality. In this study the different applications and functionalities of the program are demonstrated such as: exhaustive assembly (rDNA region and mitochondrial genome), extracting homologous regions or genes (IGS, RPB1, RPB2 and TEF1a), as well as extracting multiple regions within a single run. The program is also compared with MITObim, which is meant for the exhaustive assembly of a single target based on a similar query sequence. GRAbB is shown to be more efficient than MITObim in terms of speed, memory and disk usage. The other functionalities (handling multiple targets simultaneously and extracting homologous regions) of the new program are not matched by other programs. The program is available with explanatory documentation at https://github.com/b-brankovics/grabb. GRAbB has been tested on Ubuntu (12.04 and 14.04), Fedora (23), CentOS (7.1.1503) and Mac OS X (10.7). Furthermore, GRAbB is available as a docker repository: brankovics/grabb (https://hub.docker.com/r/brankovics/grabb/). PMID:27308864

GRAbB: Selective Assembly of Genomic Regions, a New Niche for Genomic Research.

PubMed

Brankovics, Balázs; Zhang, Hao; van Diepeningen, Anne D; van der Lee, Theo A J; Waalwijk, Cees; de Hoog, G Sybren

2016-06-01

GRAbB (Genomic Region Assembly by Baiting) is a new program that is dedicated to assemble specific genomic regions from NGS data. This approach is especially useful when dealing with multi copy regions, such as mitochondrial genome and the rDNA repeat region, parts of the genome that are often neglected or poorly assembled, although they contain interesting information from phylogenetic or epidemiologic perspectives, but also single copy regions can be assembled. The program is capable of targeting multiple regions within a single run. Furthermore, GRAbB can be used to extract specific loci from NGS data, based on homology, like sequences that are used for barcoding. To make the assembly specific, a known part of the region, such as the sequence of a PCR amplicon or a homologous sequence from a related species must be specified. By assembling only the region of interest, the assembly process is computationally much less demanding and may lead to assemblies of better quality. In this study the different applications and functionalities of the program are demonstrated such as: exhaustive assembly (rDNA region and mitochondrial genome), extracting homologous regions or genes (IGS, RPB1, RPB2 and TEF1a), as well as extracting multiple regions within a single run. The program is also compared with MITObim, which is meant for the exhaustive assembly of a single target based on a similar query sequence. GRAbB is shown to be more efficient than MITObim in terms of speed, memory and disk usage. The other functionalities (handling multiple targets simultaneously and extracting homologous regions) of the new program are not matched by other programs. The program is available with explanatory documentation at https://github.com/b-brankovics/grabb. GRAbB has been tested on Ubuntu (12.04 and 14.04), Fedora (23), CentOS (7.1.1503) and Mac OS X (10.7). Furthermore, GRAbB is available as a docker repository: brankovics/grabb (https://hub.docker.com/r/brankovics/grabb/).

Mapping of the thermal neutron distribution in the lead block assembly of the PS-211 experiment at CERN, using thermoluminescence and nuclear track detectors.

PubMed

Savvidis, E; Eleftheriadis, C A; Kitis, G

2002-01-01

The main purpose of the TARC (Transmutation by Adiabatic Resonance Crossing) experiment (PS-211), was to demonstrate the possibility to destroy efficiently Long-Lived Fission Fragments (LLFF) in Accelerator Driven Systems (ADS). The experimental set-up which consisted of a lead block with dimensions 3.3 x 3.3 x 3 m3, was installed in a CERN Proton Synchrotron (PS) beam line. The proton beam at 2.5 GeV/c and 3.5 GeV/c, was incident in the centre of the lead block assembly producing neutrons via spallation reactions. In this study, neutron flux measurements are presented in the lead block assembly using thermoluminescence and nuclear track detectors. The results are in good agreement with Monte Carlo calculations as well as with the results of the other methods used in the framework of the TARC experiment.

Formation of Polymer Particles by Direct Polymerization on the Surface of a Supramolecular Template.

PubMed

Schmuck, Carsten; Li, Mao; Zellermann, Elio

2018-04-06

Formation of polymeric materials on the surface of supramolecular assemblies is rather challenging due to the often weak non-covalent interactions between the self-assembled template and the monomers before polymerization. We herein describe that the introduction of a supramolecular anion recognition motif, the guanidiniocarbonyl pyrrole cation (GCP), into a short Fmoc-dipeptide 1 leads to self-assembled spherical nanoparticles in aqueous solution. Onto the surface of these nanoparticles negatively charged diacetylene monomers can be attached which after UV polymerization lead to the formation of a polymer shell around the self-assembled template. The hybrid supramolecular and polymeric nanoparticles demonstrated intriguing thermal hysteresis phenomenon. The template nanoparticle could be disassembled through the treatment with organic base which cleaved the Fmoc moiety on 1. This strategy thus showed that a supramolecular anion recognition motif allows the post-assembly formation of polymeric nanomaterials from anionic monomers around a cationic self-assembled template. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Novel Trisubstituted Benzimidazoles, Targeting Mtb FtsZ, As A New Class of Antitubercular Agents

PubMed Central

Kumar, Kunal; Awasthi, Divya; Lee, Seung-Yub; Zanardi, Ilaria; Ruzsicska, Bela; Knudson, Susan; Tonge, Peter J.; Slayden, Richard A.; Ojima, Iwao

2010-01-01

Libraries of novel trisubstituted benzimidazoles were created through rational drug design. A good number of these benzimidazoles exhibited promising MIC values in the range of 0.5-6 μg/mL (2-15 μM) for their antibacterial activity against Mtb H37Rv strain. Moreover, five of the lead compounds also exhibited excellent activity against clinical Mtb strains with different drug-resistance profiles. All lead compounds do not show appreciable cytotoxicity (IC50 >200 μM) against Vero cells, which inhibit Mtb FtsZ assembly in a dose dependent manner. The two lead compounds unexpectedly showed enhancement of the GTPase activity of Mtb FtsZ. The result strongly suggests that the increased GTPase activity destabilizes FtsZ assembly leading to efficient inhibition of FtsZ polymerization and filament formation. The TEM and SEM analyses of Mtb FtsZ and Mtb cells, respectively, treated with a lead compound strongly suggest that lead benzimidazoles have a novel mechanism of action on the inhibition of Mtb FtsZ assembly and Z-ring formation. PMID:21126020

Novel trisubstituted benzimidazoles, targeting Mtb FtsZ, as a new class of antitubercular agents.

PubMed

Kumar, Kunal; Awasthi, Divya; Lee, Seung-Yub; Zanardi, Ilaria; Ruzsicska, Bela; Knudson, Susan; Tonge, Peter J; Slayden, Richard A; Ojima, Iwao

2011-01-13

Libraries of novel trisubstituted benzimidazoles were created through rational drug design. A good number of these benzimidazoles exhibited promising MIC values in the range of 0.5-6 μg/mL (2-15 μM) for their antibacterial activity against Mtb H37Rv strain. Moreover, five of the lead compounds also exhibited excellent activity against clinical Mtb strains with different drug-resistance profiles. All lead compounds did not show appreciable cytotoxicity (IC(50) > 200 μM) against Vero cells, which inhibited Mtb FtsZ assembly in a dose dependent manner. The two lead compounds unexpectedly showed enhancement of the GTPase activity of Mtb FtsZ. The result strongly suggests that the increased GTPase activity destabilizes FtsZ assembly, leading to efficient inhibition of FtsZ polymerization and filament formation. The TEM and SEM analyses of Mtb FtsZ and Mtb cells, respectively, treated with a lead compound strongly suggest that lead benzimidazoles have a novel mechanism of action on the inhibition of Mtb FtsZ assembly and Z-ring formation.

Reliability of High I/O High Density CCGA Interconnect Electronic Packages under Extreme Thermal Environment

NASA Technical Reports Server (NTRS)

Ramesham, Rajeshuni

2012-01-01

This paper provides the experimental test results of advanced CCGA packages tested in extreme temperature thermal environments. Standard optical inspection and x-ray non-destructive inspection tools were used to assess the reliability of high density CCGA packages for deep space extreme temperature missions. Ceramic column grid array (CCGA) packages have been increasing in use based on their advantages such as high interconnect density, very good thermal and electrical performances, compatibility with standard surface-mount packaging assembly processes, and so on. CCGA packages are used in space applications such as in logic and microprocessor functions, telecommunications, payload electronics, and flight avionics. As these packages tend to have less solder joint strain relief than leaded packages or more strain relief over lead-less chip carrier packages, the reliability of CCGA packages is very important for short-term and long-term deep space missions. We have employed high density CCGA 1152 and 1272 daisy chained electronic packages in this preliminary reliability study. Each package is divided into several daisy-chained sections. The physical dimensions of CCGA1152 package is 35 mm x 35 mm with a 34 x 34 array of columns with a 1 mm pitch. The dimension of the CCGA1272 package is 37.5 mm x 37.5 mm with a 36 x 36 array with a 1 mm pitch. The columns are made up of 80% Pb/20%Sn material. CCGA interconnect electronic package printed wiring polyimide boards have been assembled and inspected using non-destructive x-ray imaging techniques. The assembled CCGA boards were subjected to extreme temperature thermal atmospheric cycling to assess their reliability for future deep space missions. The resistance of daisy-chained interconnect sections were monitored continuously during thermal cycling. This paper provides the experimental test results of advanced CCGA packages tested in extreme temperature thermal environments. Standard optical inspection and x-ray non-destructive inspection tools were used to assess the reliability of high density CCGA packages for deep space extreme temperature missions. Keywords: Extreme temperatures, High density CCGA qualification, CCGA reliability, solder joint failures, optical inspection, and x-ray inspection.

1. MISSILE TEST AND ASSEMBLY BUILDING, FRONT, LOOKING SOUTH. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

1. MISSILE TEST AND ASSEMBLY BUILDING, FRONT, LOOKING SOUTH. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

Swing-arm beam erector (SABER) concept for single astronaut assembly of space structure

NASA Technical Reports Server (NTRS)

Watson, J. J.; Heard, W. L., Jr.; Jensen, J. K.

1985-01-01

Results are presented of tests conducted to evaluate a mobile work station/assembly fixture concept that would mechanically assist an astronaut in the on-orbit manual assembly of erectable truss-beams. The concept eliminates astronaut manual translation by use of a motorized work platform with foot restraints. The tests involved assembly of a tetrahedral truss-beam by a test subject in simulated zero gravity (neutral bouyancy in water). A three-bay truss-beam was assembled from 30 aluminum struts with quick-attachment structural joints. The results show that average on-orbit assembly rates of 2.1 struts per minute can be expected for struts of the size employed in these tests.

L2F anemometry measurements of the inter-row flow field within a transonic axial compressor provided with curved windows

NASA Astrophysics Data System (ADS)

Ottavy, Xavier; Trébinjac, Isabelle; Vouillarmet, André

1999-09-01

When measurements are performed in high speed, small-scale compressors, the use of curved glass windows is required in order to avoid any mismatch between the measurement window and the casing. However, the glass curvature leads to optical distortions, which hinder acceptable measurements and can even prevent the acquisition of any data. Thus, an original optical assembly, which consists in inserting a simple and inexpensive corrective window between the frontal lens of the anemometer and the shroud window, is proposed. The way of determining the geometric characteristics and the position of this corrective window, which restores very acceptable foci, is presented in the paper. The reliability of this corrective optical assembly is highlighted by comparative measurements in a test case. Using such an optical setting, L2F measurements were realised along a section, downstream of the inlet guide vane (IGV) of a transonic compressor stage. The spatial resolution leads to a good description of the interaction of the wake with the oblique shock emanating from the leading edge of the rotor. A phenomenological study of the wake/shock interaction with a change of frame is realised using the streamwise equation of the transport of vorticity.

A Clear Success for International Transport of Plutonium and MOX Fuels

DOE Office of Scientific and Technical Information (OSTI.GOV)

Blachet, L.; Jacot, P.; Bariteau, J.P.

2006-07-01

An Agreement between the United States and Russia to eliminate 68 metric tons of surplus weapons-grade plutonium provided the basis for the United States government and its agency, the Department of Energy (DOE), to enter into contracts with industry leaders to fabricate mixed oxide (MOX) fuels (a blend of uranium oxide and plutonium oxide) for use in existing domestic commercial reactors. DOE contracted with Duke, COGEMA, Stone and Webster (DCS), a limited liability company comprised of Duke Energy, COGEMA Inc. and Stone and Webster to design a Mixed Oxide Fuel Fabrication Facility (MFFF) which would be built and operated atmore » the DOE Savannah River Site (SRS) near Aiken, South Carolina. During this same time frame, DOE commissioned fabrication and irradiation of lead test assemblies in one of the Mission Reactors to assist in obtaining NRC approval for batch implementation of MOX fuel prior to the operations phase of the MFFF facility. On February 2001, DOE directed DCS to initiate a pre-decisional investigation to determine means to obtain lead assemblies including all international options for manufacturing MOX fuels. This lead to implementation of the EUROFAB project and work was initiated in earnest on EUROFAB by DCS on November 7, 2003. (authors)« less

Tuning carbon nanotube assembly for flexible, strong and conductive films.

PubMed

Wang, Yanjie; Li, Min; Gu, Yizhuo; Zhang, Xiaohua; Wang, Shaokai; Li, Qingwen; Zhang, Zuoguang

2015-02-21

Carbon nanotubes are ideal scaffolds for designing and architecting flexible graphite films with tunable mechanical, electrical and thermal properties. Herein, we demonstrate that the assembly of aligned carbon nanotubes with different aggregation density and morphology leads to different mechanical properties and anisotropic electrical conduction along the films. Using drying evaporation under tension treatment, the carbon nanotubes can be assembled into strong films with tensile strength and Young's modulus as high as 3.2 GPa and 124 GPa, respectively, leading to a remarkable toughness of 54.38 J g(-1), greatly outperforming conventional graphite films, spider webs and even Kevlar fiber films. Different types of solvents may result in the assembly of CNTs with different aggregation morphology and therefore different modulus. In addition, we reveal that the high density assembly of aligned CNTs correlates with better electric conduction along the axial direction, enabling these flexible graphite films to be both strong and conductive.

Pyridyl-Amides as a Multimode Self-Assembly Driver for the Design of a Stimuli-Responsive π-Gelator.

PubMed

Kartha, Kalathil K; Praveen, Vakayil K; Babu, Sukumaran Santhosh; Cherumukkil, Sandeep; Ajayaghosh, Ayyappanpillai

2015-10-01

An oligo(p-phenylenevinylene) (OPV) derivative connected to pyridyl end groups through an amide linkage (OPV-Py) resulted in a multistimuli-responsive π-gelator. When compared to the corresponding OPV π-gelator terminated by a phenyl-amide (OPV-Ph), the aggregation properties of OPV-Py were found to be significantly different, leading to multistimuli gelation and other morphological properties. The pyridyl moiety in OPV-Py initially interferes with the amide H-bonded assembly and gelation, however, protonation of the pyridyl moiety with trifluoroacetic acid (TFA) facilitated the formation of amide H-bonded assembly leading to gelation, which is reversible by the addition of N,N-diisopropyethylamine (DiPEA). Interestingly, addition of Ag(+) ions to a solution of OPV-Py facilitated the formation of a metallo-supramolecular assembly leading to gelation. Surprisingly, ultrasound-induced gelation was observed when OPV-Py was mixed with a dicarboxylic acid (A1). A detailed study using different spectroscopic and microscopic experimental techniques revealed the difference in the mode of assembly in the two molecules and the multistimuli-responsive nature of the OPV-Py gelation. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Cryogenic experiences during W7-X HTS-current lead tests

DOE Office of Scientific and Technical Information (OSTI.GOV)

Richter, Thomas; Lietzow, Ralph

2014-01-29

The Karlsruhe Institute of Technology (KIT) was responsible for design, production and test of the High Temperature Superconductor (HTS) current leads (CL) for the stellerator Wendelstein 7-X (W7-X). 16 current leads were delivered. Detailed prototype tests as well as the final acceptance tests were performed at KIT, using a dedicated test cryostat assembled beside and connected to the main vacuum vessel of the TOSKA facility. A unique feature is the upside down orientation of the current leads due to the location of the power supplies in the basement of the experimental area of W7-X. The HTS-CL consists of three mainmore » parts: the cold end for the connection to the bus bar at 4.5 K, the HTS part operating in the temperature range from 4.5 K to 65 K and a copper heat exchanger (HEX) in the temperature range from 65 K to room temperature, which is cooled with 50 K helium. Therefore in TOSKA it is possible to cool test specimens simultaneously with helium at two different temperature levels. The current lead tests included different scenarios with currents up to 18.2 kA. In total, 10 cryogenic test campaigns with a total time of about 24 weeks were performed till beginning of 2013. The test facility as well as the 2 kW cryogenic plant of ITEP showed a very good reliability. However, during such a long and complex experimental campaign, one has to deal with failures, technical difficulties and incidents. The paper gives a summary of the test performance comprising the test preparation and operation. This includes the performance and reliability of the refrigerator and the test facility with reference to the process measuring and control system, the data acquisition system, as well as the building infrastructure.« less

4. MISSILE TEST AND ASSEMBLY BUILDING, LEFT SIDE, LOOKING NORTH. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. MISSILE TEST AND ASSEMBLY BUILDING, LEFT SIDE, LOOKING NORTH. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

2. MISSILE TEST AND ASSEMBLY BUILDING, RIGHT SIDE, LOOKING WEST. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. MISSILE TEST AND ASSEMBLY BUILDING, RIGHT SIDE, LOOKING WEST. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

3. MISSILE TEST AND ASSEMBLY BUILDING, REAR SIDE, LOOKING NORTH. ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

3. MISSILE TEST AND ASSEMBLY BUILDING, REAR SIDE, LOOKING NORTH. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

Toward a mechanistic understanding of the damage evolution of SnAgCu solder joints in accelerated thermal cycling test

NASA Astrophysics Data System (ADS)

Mahin Shirazi, Sam

Accelerated thermal cycling (ATC) tests are the most commonly used tests for the thermo-mechanical performance assessment of microelectronics assemblies. Currently used reliability models have failed to incorporate the microstructural dependency of lead free solder joint behavior and its microstructure evolution during cycling. Thus, it is essential to have a mechanistic understanding of the effect of cycling parameters on damage evolution and failure of lead free solder joints in ATC. Recrystallization has been identified as the damage rate controlling mechanism in ATC. Usually it takes 1/3 of life for completion of recrystallization regardless of cycling parameters. Thus, the life of the solder joints can be predicted by estimating global recrystallization. The objective of the first part of the study was to examine whether the damage scenario applies in service is the same as the harsh thermal cycling tests (i.e. 0/100 °C and -40/125 °C) commonly used in industry. Microstructure analysis results on a variety of lead free solder SnAgCu assemblies subjected to the both harsh (0/100 °C) and mild (20/80 °C) ATC confirmed similar failure mechanism under the both testing conditions. Sn grain morphology (interlaced versus beach ball) has a significant effect on the thermo-mechanical performance (and thus the model) of the lead free solder joints. The longer thermal cycling lifetime observed in the interlaced solder joints subjected to the ATC compared to the beach ball structure was correlated to the different initial microstructure and the microstructure evolution during cycling. For the modeling proposes, the present study was focused on Sn-Ag-Cu solder joints with either a single Sn grain or beach ball structure. Microstructural analysis results of the simulated thermal cycling experiment revealed that, the life can be approximated as determined by the accumulation of a certain amount of work during the high temperature dwells. Finally the effect of precipitates spacing on acceleration factor was investigated. Results indicated that a smaller initial precipitate spacing would tend to result in a longer life in mild thermal cycling/service (where there is lower stresses). Accordingly, it is essential to incorporate the dependence of damage rate (i.e. recrystallization) on precipitate coarsening in any predictions.

78 FR 21607 - Stakeholder Listening Session in Preparation for the 66th World Health Assembly

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-11

... World Health Assembly Time and date: May 6, 2013, 3 p.m.-4:30 p.m. EST. Place: Great Hall of the Hubert... Human Services (HHS)--charged with leading the U.S. delegation to the 66th World Health Assembly-- will... Listening Session will help the HHS's Office of Global Affairs prepare for the World Health Assembly by...

77 FR 19666 - Stakeholder Listening Session in Preparation for the 65th World Health Assembly

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-02

... World Health Assembly Time and Date: April 30, 2012, 3 p.m.-4:30 p.m. EST. Place: Great Hall of the... with leading the U.S. delegation to the 65th World Health Assembly-- will hold an informal Stakeholder... HHS's Office of Global Affairs prepare for the World Health Assembly by taking full advantage of the...

Berthing mechanism final test report and program assessment

NASA Technical Reports Server (NTRS)

1988-01-01

The purpose is to document the testing performed on both hardware and software developed under the Space Station Berthing Mechanisms Program. Testing of the mechanism occurred at three locations. Several system components, e.g., actuators and computer systems, were functionally tested before assembly. A series of post assembly tests were performed. The post assembly tests, as well as the dynamic testing of the mechanism, are presented.

49 CFR 572.145 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) The test objective is to determine the resistance of the... upper and lower halves of the torso assembly (refer to § 572.140(a)(1)(iv)). (b)(1) When the upper half...

49 CFR 572.145 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) The test objective is to determine the resistance of the... upper and lower halves of the torso assembly (refer to § 572.140(a)(1)(iv)). (b)(1) When the upper half...

49 CFR 572.145 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) The test objective is to determine the resistance of the... upper and lower halves of the torso assembly (refer to § 572.140(a)(1)(iv)). (b)(1) When the upper half...

49 CFR 572.145 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Upper and lower torso assemblies and torso flexion... assemblies and torso flexion test procedure. (a) The test objective is to determine the resistance of the... upper and lower halves of the torso assembly (refer to § 572.140(a)(1)(iv)). (b)(1) When the upper half...

SSME component assembly and life management expert system

NASA Technical Reports Server (NTRS)

Ali, M.; Dietz, W. E.; Ferber, H. J.

1989-01-01

The space shuttle utilizes several rocket engine systems, all of which must function with a high degree of reliability for successful mission completion. The space shuttle main engine (SSME) is by far the most complex of the rocket engine systems and is designed to be reusable. The reusability of spacecraft systems introduces many problems related to testing, reliability, and logistics. Components must be assembled from parts inventories in a manner which will most effectively utilize the available parts. Assembly must be scheduled to efficiently utilize available assembly benches while still maintaining flight schedules. Assembled components must be assigned to as many contiguous flights as possible, to minimize component changes. Each component must undergo a rigorous testing program prior to flight. In addition, testing and assembly of flight engines and components must be done in conjunction with the assembly and testing of developmental engines and components. The development, testing, manufacture, and flight assignments of the engine fleet involves the satisfaction of many logistical and operational requirements, subject to many constraints. The purpose of the SSME Component Assembly and Life Management Expert System (CALMES) is to assist the engine assembly and scheduling process, and to insure that these activities utilize available resources as efficiently as possible.

Highly luminescent colloidal nanoplates of perovskite cesium lead halide and their oriented assemblies

DOE PAGES

Bekenstein, Yehonadav; Koscher, Brent A.; Eaton, Samuel W.; ...

2015-12-15

Anisotropic colloidal quasi-two-dimensional nanoplates (NPLs) hold great promise as functional materials due to their combination of low dimensional optoelectronic properties and versatility through colloidal synthesis. Recently, lead-halide perovskites have emerged as important optoelectronic materials with excellent efficiencies in photovoltaic and light-emitting applications. Here we report the synthesis of quantum confined all inorganic cesium lead halide nanoplates in the perovskite crystal structure that are also highly luminescent (PLQY 84%). The controllable self-assembly of nanoplates either into stacked columnar phases or crystallographic-oriented thin-sheet structures is demonstrated. Furthermore, the broad accessible emission range, high native quantum yields, and ease of self-assembly make perovskitemore » NPLs an ideal platform for fundamental optoelectronic studies and the investigation of future devices.« less

Neutral buoyancy test evaluation of hardware and extravehicular activity procedures for on-orbit assembly of a 14 meter precision reflector

NASA Technical Reports Server (NTRS)

Heard, Walter L., Jr.; Lake, Mark S.

1993-01-01

A procedure that enables astronauts in extravehicular activity (EVA) to perform efficient on-orbit assembly of large paraboloidal precision reflectors is presented. The procedure and associated hardware are verified in simulated Og (neutral buoyancy) assembly tests of a 14 m diameter precision reflector mockup. The test article represents a precision reflector having a reflective surface which is segmented into 37 individual panels. The panels are supported on a doubly curved tetrahedral truss consisting of 315 struts. The entire truss and seven reflector panels were assembled in three hours and seven minutes by two pressure-suited test subjects. The average time to attach a panel was two minutes and three seconds. These efficient assembly times were achieved because all hardware and assembly procedures were designed to be compatible with EVA assembly capabilities.

5. MISSILE TEST AND ASSEMBLY BUILDING, FRONT AND RIGHT SIDES, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

5. MISSILE TEST AND ASSEMBLY BUILDING, FRONT AND RIGHT SIDES, LOOKING SOUTHEAST. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

6. MISSILE TEST AND ASSEMBLY BUILDING, REAR AND LEFT SIDES, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

6. MISSILE TEST AND ASSEMBLY BUILDING, REAR AND LEFT SIDES, LOOKING NORTHWEST. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

Optimal Testlet Pool Assembly for Multistage Testing Designs

ERIC Educational Resources Information Center

Ariel, Adelaide; Veldkamp, Bernard P.; Breithaupt, Krista

2006-01-01

Computerized multistage testing (MST) designs require sets of test questions (testlets) to be assembled to meet strict, often competing criteria. Rules that govern testlet assembly may dictate the number of questions on a particular subject or may describe desirable statistical properties for the test, such as measurement precision. In an MST…

KSC-2012-6222

NASA Image and Video Library

2012-11-09

CAPE CANAVERAL, Fla. -- At the Neo Liquid Propellant Testbed inside a facility near Kennedy Space Center’s Shuttle Landing Facility in Florida, engineers and Rocket University project leads Kyle Dixon, left, and Evelyn Orozco-Smith check the buildup of the Neo test fixture and an Injector 71 engine that uses super-cooled propellants. NASA engineers are working on the design and assembly of the Neo Liquid Propellant Testbed as part of the Engineering Directorate’s Rocket University training program. Photo credit: NASA/Frankie Martin

KENNEDY SPACE CENTER, FLA. -Crawler-transporter (CT) number 2, moves away from the Vehicle Assembly Building, with a Mobile Launcher Platform on top, on a test run to the launch pad. The CT recently underwent modifications to the cab. The CT moves Space Shuttle vehicles between the VAB and launch pad. Moving on four double-tracked crawlers, the CT uses a laser guidance system and a leveling system for the journey that keeps the top of a Space Shuttle vertical within plus- or minus-10 minutes of arc. The system enables the CT-MLP-Shuttle to negotiate the ramp leading to the launch pads and keep the load level. Unloaded, the CT weighs 6 million pounds.

NASA Image and Video Library

2003-08-18

KENNEDY SPACE CENTER, FLA. -Crawler-transporter (CT) number 2, moves away from the Vehicle Assembly Building, with a Mobile Launcher Platform on top, on a test run to the launch pad. The CT recently underwent modifications to the cab. The CT moves Space Shuttle vehicles between the VAB and launch pad. Moving on four double-tracked crawlers, the CT uses a laser guidance system and a leveling system for the journey that keeps the top of a Space Shuttle vertical within plus- or minus-10 minutes of arc. The system enables the CT-MLP-Shuttle to negotiate the ramp leading to the launch pads and keep the load level. Unloaded, the CT weighs 6 million pounds.

A Library of the Nanoscale Self-Assembly of Amino Acids on Metal Surfaces

NASA Astrophysics Data System (ADS)

Iski, Erin; Yitamben, Esmeralda; Guisinger, Nathan

2012-02-01

The investigation of the hierarchical self-assembly of amino acids on surfaces represents a unique test-bed for the origin of enantio-favoritism in biology and the transmission of chirality from single molecules to complete surface layers. These chiral systems, in particular the assembly of isoleucine and alanine on Cu(111), represent a direct link to the understanding of certain biological processes, specifically the preference for some amino acids to form alpha helices vs. beta-pleated sheets in the secondary structure of proteins. Low temperature, ultra-high vacuum, scanning tunneling microscopy (LT UHV-STM) is used to study the hierarchical self-assembly of different amino acids on a Cu(111) single crystal in an effort to build a library of their two-dimensional structure with molecular-scale resolution for enhanced protein and peptide studies. Both enantiopure and racemic structures are studied in order to elucidate how chirality can affect the self-assembly of the amino acids. In some cases, density functional theory (DFT) models can be used to confirm the experimental structure. The advent of such a library with fully resolved, two-dimensional structures at different molecular coverages would address some of the complex questions surrounding the preferential formation of alpha helices vs. beta-pleated sheets in proteins and lead to a better understanding of the key role played by these amino acids in protein sequencing.

Method and apparatus for advancing tethers

DOEpatents

Zollinger, W. Thor

1998-01-01

A tether puller for advancing a tether through a channel may include a bellows assembly having a leading end fixedly attached to the tether at a first position and a trailing end fixedly attached to the tether at a second position so that the leading and trailing ends of the bellows assembly are located a substantially fixed distance apart. The bellows assembly includes a plurality of independently inflatable elements each of which may be separately inflated to an extended position and deflated to a retracted position. Each of the independently inflatable elements expands radially and axially upon inflation. An inflation system connected to the independently inflatable elements inflates and deflates selected ones of the independently inflatable elements to cause the bellows assembly to apply a tractive force to the tether and advance it in the channel.

Polymeric amphiphile branching leads to rare nanodisc shaped planar self-assemblies.

PubMed

Qu, Xiaozhong; Omar, Leila; Le, Thi Bich Hang; Tetley, Laurence; Bolton, Katherine; Chooi, Kar Wai; Wang, Wei; Uchegbu, Ijeoma F

2008-09-16

Self-assembly is fundamental to the biological function of cells and the fabrication of nanomaterials. However, the origin of the shape of various self-assemblies, such as the shape of cells, is not altogether clear. Polymeric, oligomeric, or low molecular weight amphiphiles are a rich source of nanomaterials, and controlling their self-assembly is the route to tailored nanosystems with specific functionalities. Here, we provide direct evidence that a particular molecular architecture, polymeric branching, leads to a rare form of self-assembly, the planar nanodisc. Cholesterol containing self-assemblies formed from amphiphilic linear or branched cetyl poly(ethylenimine) (Mn approximately 1000 Da) or amphiphilic cetyl poly(propylenimine) dendrimer derivatives (Mn approximately 2000 Da) show that branching, by reducing the hydrophilic headgroup area, alters the shape of the self-assemblies transforming closed 60 nm spherical bilayer vesicles to rare 50 nm x 10 nm planar bilayer discs. Increasing the hydrophilic headgroup area, by the inclusion of methoxy poly(ethylene glycol) moieties into the amphiphilic headgroup, transforms the planar discs to 100 nm spherical bilayer vesicles. This study provides insight into the key role played by molecular shape on molecular self-organization into rare nanodiscs.

Experiments to evolve toward a tangible user interface for computer-aided design parts assembly

NASA Astrophysics Data System (ADS)

Legardeur, Jeremy; Garreau, Ludovic; Couture, Nadine

2004-05-01

In this paper, we present the concepts of the ESKUA (Experimentation of a Kinesics System Usable for Assembly) platform that allows designers to carry out the assembly of mechanical CAD (Computer Aided Design) parts. This platform, based on tangible user interface lead taking into account assembly constraints from the beginning of the design phase and especially during the phase of CAD models manipulation. Our goal is to propose a working environment where the designer is confronted with real assembly constraints which are currently masked by existing CAD software functionalities. Thus, the platform is based on the handling of physical objects, called tangible interactors, which enable having a physical perception of the assembly constraints. In this goal, we have defined a typology of interactors based on concepts proposed in Design For Assembly methods. We present here the results of studies that led to the evolution of this first interactors set. One is concerning an experiment to evaluate the cognitive aspects of the use of interactors. The other is about an analysis of existing mechanical product and fasteners. We will show how these studies lead to the evolution of the interactors based on the functional surfaces use.

Surrogate fuel assembly multi-axis shaker tests to simulate normal conditions of rail and truck transport

DOE Office of Scientific and Technical Information (OSTI.GOV)

McConnell, Paul E.; Koenig, Greg John; Uncapher, William Leonard

2016-05-01

This report describes the third set of tests (the “DCLa shaker tests”) of an instrumented surrogate PWR fuel assembly. The purpose of this set of tests was to measure strains and accelerations on Zircaloy-4 fuel rods when the PWR assembly was subjected to rail and truck loadings simulating normal conditions of transport when affixed to a multi-axis shaker. This is the first set of tests of the assembly simulating rail normal conditions of transport.

Surrogate fuel assembly multi-axis shaker tests to simulate normal conditions of rail and truck transport

DOE Office of Scientific and Technical Information (OSTI.GOV)

McConnell, Paul E.; Koenig, Greg John; Uncapher, William Leonard

2016-05-12

This report describes the third set of tests (the “DCL a shaker tests”) of an instrumented surrogate PWR fuel assembly. The purpose of this set of tests was to measure strains and accelerations on Zircaloy-4 fuel rods when the PWR assembly was subjected to rail and truck loadings simulating normal conditions of transport when affixed to a multi-axis shaker. This is the first set of tests of the assembly simulating rail normal conditions of transport.

Committee Meeting of Assembly Education Committee "To Receive Testimony from the Commissioner of Education, Mary Lee Fitzgerald, Department Staff, and Others Concerning the Department's Skills Testing Program, Including the Early Warning Test and High School Proficiency Test, Pursuant to Assembly Resolution No. 113."

ERIC Educational Resources Information Center

New Jersey State Office of Legislative Services, Trenton. Assembly Education Committee.

The Assembly Education Committee of the New Jersey Office of Legislative Services held a hearing pursuant to Assembly Resolution 113, a proposal directing the Committee to investigate the skills testing program developed and administered to New Jersey children by the State Department of Education. The Committee was interested in the eighth-grade…

A System for the Automatic Assembly of Test Questions Using a No-SQL Database

ERIC Educational Resources Information Center

Shin, Sanggyu; Hashimoto, Hiroshi

2014-01-01

We describe a system that automatically assembles test questions from a set of examples. Our system can create test questions appropriate for each user's level at low cost. In particular, when a user review their lesson, our system provides new test questions which are assembled based on their previous test results and past mistakes, rather than a…

Micro electro-mechanical heater

DOEpatents

Oh, Yunje; Asif, Syed Amanulla Syed; Cyrankowski, Edward; Warren, Oden Lee

2016-04-19

A sub-micron scale property testing apparatus including a test subject holder and heating assembly. The assembly includes a holder base configured to couple with a sub-micron mechanical testing instrument and electro-mechanical transducer assembly. The assembly further includes a test subject stage coupled with the holder base. The test subject stage is thermally isolated from the holder base. The test subject stage includes a stage subject surface configured to receive a test subject, and a stage plate bracing the stage subject surface. The stage plate is under the stage subject surface. The test subject stage further includes a heating element adjacent to the stage subject surface, the heating element is configured to generate heat at the stage subject surface.

Micro electro-mechanical heater

DOEpatents

Oh, Yunje; Asif, Syed Amanulla Syed; Cyrankowski, Edward; Warren, Oden Lee

2017-09-12

A sub-micron scale property testing apparatus including a test subject holder and heating assembly. The assembly includes a holder base configured to couple with a sub-micron mechanical testing instrument and electro-mechanical transducer assembly. The assembly further includes a test subject stage coupled with the holder base. The test subject stage is thermally isolated from the holder base. The test subject stage includes a stage subject surface configured to receive a test subject, and a stage plate bracing the stage subject surface. The stage plate is under the stage subject surface. The test subject stage further includes a heating element adjacent to the stage subject surface, the heating element is configured to generate heat at the stage subject surface.

HOWARD EISEN, JPL'S LEAD MECHANICAL TECHNICIAN, HOLDS MARS PATHFINDER 'SOJOURNER' ROVER 1:1 SCALE DU

NASA Technical Reports Server (NTRS)

1996-01-01

The Mars Pathfinder 'Sojourner' rover l:l scale duplicate test vehicle is held by Howard Eisen, its lead mechanical technician from the Jet Propulsion Laboratory, with Kennedy Space Center's Vehicle Assembly Building looming in the background. The launch of NASA's Mars Pathfinder spacecraft aboard a McDonnell Douglas Delta II rocket is scheduled for Monday, Dec. 2, at 2:09:11 a.m. EST. This is a single instantaneous target launch time without a second opportunity on that day. Liftoff will occur from Pad B at Launch Complex 17 on Cape Canaveral Air Station, Fla. There is a 24-day launch opportunity which extends through Dec. 31.

Lead free 0201 assembly and thermal cycle/aging reliability

NASA Technical Reports Server (NTRS)

Ghaffarian, Reza; Ramkumar, S. Manian; Varanasi, Arun

2005-01-01

The many challenges with 0201 passive component assembly can be attributed to the solder paste volume, pad design, aperture design, board finish, type of solder paste, pick-and-place, and reflow profile. A Design-of-Experiment (DOE) study was carried out to investigate the effects of these parameters on assembly defects and reliability.

78 FR 33197 - Airworthiness Directives; Iniziative Industriali Italiane S.p.A. Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-04

... plane hinge assembly. We are issuing this AD to require actions to address the unsafe condition on these... plane hinge assembly have been reported. This condition, if not detected and corrected, could lead to... bearing and the horizontal tail/elevator plane hinge assembly to detect any crack, signs of corrosion or...

Bending nanofibers into nanospirals: coordination chemistry as a tool for shaping hydrophobic assemblies.

PubMed

Kossoy, Elizaveta; Weissman, Haim; Rybtchinski, Boris

2015-01-02

In the current work, we demonstrate how coordination chemistry can be employed to direct self-assembly based on strong hydrophobic interactions. To investigate the influence of coordination sphere geometry on aqueous self-assembly, we synthesized complexes of the amphiphilic perylene diimide terpyridine ligand with the first-row transition-metal centers (zinc, cobalt, and nickel). In aqueous medium, aggregation of these complexes is induced by hydrophobic interactions between the ligands. However, the final shapes of the resulting assemblies depend on the preferred geometry of the coordination spheres typical for the particular metal center. The self-assembly process was characterized by UV/Vis spectroscopy, zeta potential measurements, and cryogenic transmission electron microscopy (cryo-TEM). Coordination of zinc(II) and cobalt(II) leads to the formation of unique nanospiral assemblies, whereas complexation of nickel(II) leads to the formation of straight nanofibers. Notably, coordination bonds are utilized not as connectors between elementary building blocks, but as directing interactions, enabling control over supramolecular geometry. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Ebola virus VP35 blocks stress granule assembly.

PubMed

Le Sage, Valerie; Cinti, Alessandro; McCarthy, Stephen; Amorim, Raquel; Rao, Shringar; Daino, Gian Luca; Tramontano, Enzo; Branch, Donald R; Mouland, Andrew J

2017-02-01

Stress granules (SGs) are dynamic cytoplasmic aggregates of translationally silenced mRNAs that assemble in response to environmental stress. SGs appear to play an important role in antiviral innate immunity and many viruses have evolved to block or subvert SGs components for their own benefit. Here, we demonstrate that intracellular Ebola virus (EBOV) replication and transcription-competent virus like particles (trVLP) infection does not lead to SG assembly but leads to a blockade to Arsenite-induced SG assembly. Moreover we show that EBOV VP35 represses the assembly of canonical and non-canonical SGs induced by a variety of pharmacological stresses. This SG blockade requires, at least in part, the C-terminal domain of VP35. Furthermore, results from our co-immunoprecipitation studies indicate that VP35 interacts with multiple SG components, including G3BP1, eIF3 and eEF2 through a stress- and RNA-independent mechanism. These data suggest a novel function for EBOV VP35 in the repression of SG assembly. Copyright © 2016 Elsevier Inc. All rights reserved.

Fabrication of capsule assemblies, phase 3

NASA Technical Reports Server (NTRS)

Keeton, A. R.; Stemann, L. G.

1973-01-01

Thirteen capsule assemblies were fabricated for evaluation of fuel pin design concepts for a fast spectrum lithium cooled compact space power reactor. These instrumented assemblies were designed for real time test of prototype fuel pins. Uranium mononitride fuel pins were encased in AISI 304L stainless steel capsules. Fabrication procedures were fully qualified by process development and assembly qualification tests. Instrumentation reliability was achieved utilizing specially processed and closely controlled thermocouple hot zone fabrication and by thermal screening tests. Overall capsule reliability was achieved with an all electron beam welded assembly.

Process for Testing Compaction of a Swaged Heater for an Anode Sub-Assembly of a Hollow Cathode Assembly

NASA Technical Reports Server (NTRS)

Patterson, Michael J. (Inventor); Verhey, Timothy R. R. (Inventor); Soulas, George C. (Inventor)

2003-01-01

A process for testing compaction of a swaged heater for an anode sub-assembly of a Hollow Cathode Assembly (HCA), in which a test sample is cleaned, its mass measured before and after immersion in kerosene for 24 hours, and a compaction percentage calculated. A swaged heater is rejected if the compaction percentage exceeds 84%, plus or minus 4%.

Assemby, test, and launch operations for the Mars Exploration Rovers

NASA Technical Reports Server (NTRS)

Wallace, Matthew T.; Hardy, Paul V.; Romero, Raul A.; Salvo, Christopher G.; Shain, Thomas W.; Thompson, Arthur D.; Wirth, John W.

2005-01-01

In January of 2004, NASA's twin Mars rovers, Spirit and Opportunity, successfully landed on opposite sides of the Red Planet after a seven month Earth to Mars cruise period. Both vehicles have operated well beyond their 90 day primary mission design life requirements. The Assembly, Test, and Launch Operations (ATLO) program for these missions presented unique technical and schedule challenges to the team at the Jet Propulsion Laboratory (JPL). Among these challenges were a highly compressed schedule and late deliveries leading to extended double shift staffing, dual spacecraft operations requiring test program diversification and resource arbitration, multiple atypical test configurations for airbag/rocket landings and surface mobility testing, and verification of an exceptionally large number of separations, deployments, and mechanisms. This paper discusses the flight system test philosophies and approach, and presents lessons learned.

Uncertainties in the Item Parameter Estimates and Robust Automated Test Assembly

ERIC Educational Resources Information Center

Veldkamp, Bernard P.; Matteucci, Mariagiulia; de Jong, Martijn G.

2013-01-01

Item response theory parameters have to be estimated, and because of the estimation process, they do have uncertainty in them. In most large-scale testing programs, the parameters are stored in item banks, and automated test assembly algorithms are applied to assemble operational test forms. These algorithms treat item parameters as fixed values,…

Electrochemical behavior of negative electrode of lead-acid cells based on reticulated vitreous carbon carrier

NASA Astrophysics Data System (ADS)

Czerwiński, A.; Obrębowski, S.; Kotowski, J.; Rogulski, Z.; Skowroński, J. M.; Krawczyk, P.; Rozmanowski, T.; Bajsert, M.; Przystałowski, M.; Buczkowska-Biniecka, M.; Jankowska, E.; Baraniak, M.

Reticulated vitreous carbon (RVC ®) and RVC ® plated with lead were investigated as carriers for the negative electrode of lead-acid cell. The RVC ® and Pb/RVC ® carriers were pasted with active paste (received from JENOX Ltd., Polish producer of lead-acid batteries) and prepared to be used in lead-acid cell. Comparative study of electrodes based on RVC ® and Pb/RVC ® has been done using constant-current charging/discharging, constant-potential discharging and cycling voltammetry measurements. Scanning electron microscopy (SEM) was employed to determine the morphology of the lead layer deposited on the RVC surface. Hybrid flooded single lead-acid cells containing one negative electrode, based on new type of carrier (RVC ® or Pb/RVC ®), sandwiched between two positive electrodes, based on the Pb-Ca grids, were assembled and subjected to electrochemical tests. It has been found that both materials, RVC ® and Pb/RVC ®, can be used as carriers of negative electrode, but the latter seems to have better influence on the discharge performance.

Brassboard Astrometric Beam Combiner (ABC) Development for the Space Interferometry Mission (SIM)

NASA Technical Reports Server (NTRS)

Jeganathan, Muthu; Kuan, Gary; Rud, Mike; Lin, Sean; Sutherland, Kristen; Moore, James; An, Xin

2008-01-01

The Astrometric Beam Combiner (ABC) is a critical element of the Space Interferometry Mission (SIM) that performs three key functions: coherently combine starlight from two siderostats; individually detect starlight for angle tracking; and disperse and detect the interferometric fringes. In addition, the ABC contains: a stimulus, cornercubes and shutters for in-orbit calibration; several tip/tilt mirror mechanisms for in-orbit alignment; and internal metrology beam launcher for pathlength monitoring. The detailed design of the brassboard ABC (which has the form, fit and function of the flight unit) is complete, procurement of long-lead items is underway, and assembly and testing is expected to be completed in Spring 2009. In this paper, we present the key requirements for the ABC, details of the completed optical and mechanical design as well as plans for assembly and alignment.

Tuning carbon nanotube assembly for flexible, strong and conductive films

NASA Astrophysics Data System (ADS)

Wang, Yanjie; Li, Min; Gu, Yizhuo; Zhang, Xiaohua; Wang, Shaokai; Li, Qingwen; Zhang, Zuoguang

2015-02-01

Carbon nanotubes are ideal scaffolds for designing and architecting flexible graphite films with tunable mechanical, electrical and thermal properties. Herein, we demonstrate that the assembly of aligned carbon nanotubes with different aggregation density and morphology leads to different mechanical properties and anisotropic electrical conduction along the films. Using drying evaporation under tension treatment, the carbon nanotubes can be assembled into strong films with tensile strength and Young's modulus as high as 3.2 GPa and 124 GPa, respectively, leading to a remarkable toughness of 54.38 J g-1, greatly outperforming conventional graphite films, spider webs and even Kevlar fiber films. Different types of solvents may result in the assembly of CNTs with different aggregation morphology and therefore different modulus. In addition, we reveal that the high density assembly of aligned CNTs correlates with better electric conduction along the axial direction, enabling these flexible graphite films to be both strong and conductive.Carbon nanotubes are ideal scaffolds for designing and architecting flexible graphite films with tunable mechanical, electrical and thermal properties. Herein, we demonstrate that the assembly of aligned carbon nanotubes with different aggregation density and morphology leads to different mechanical properties and anisotropic electrical conduction along the films. Using drying evaporation under tension treatment, the carbon nanotubes can be assembled into strong films with tensile strength and Young's modulus as high as 3.2 GPa and 124 GPa, respectively, leading to a remarkable toughness of 54.38 J g-1, greatly outperforming conventional graphite films, spider webs and even Kevlar fiber films. Different types of solvents may result in the assembly of CNTs with different aggregation morphology and therefore different modulus. In addition, we reveal that the high density assembly of aligned CNTs correlates with better electric conduction along the axial direction, enabling these flexible graphite films to be both strong and conductive. Electronic supplementary information (ESI) available: The TEM image of array CNTs. The surface height curves of x-z cross-section of the films. A comparison of the mechanical properties of the pure CNT films described in this work with other CNT films/fibers spun from CNT array reported in the literature. The measured evaporation rates of ethanol and acetone. See DOI: 10.1039/c4nr06401a

Development of a Cryogenic Thermal Distortion Measurement Facility for Testing the James Webb Space Telescope Instrument Support Integration Module 2-D Test Assemblies

NASA Technical Reports Server (NTRS)

Miller, Franklin; Bagdanove, paul; Blake, Peter; Canavan, Ed; Cofie, Emmanuel; Crane, J. Allen; Dominquez, Kareny; Hagopian, John; Johnston, John; Madison, Tim;

Phospholipid—polymer amphiphile hybrid assemblies and their interaction with macrophages

PubMed Central

Panneerselvam, Karthiga; Lynge, Martin E.; Riber, Camilla Frich; Mena-Hernando, Sofia; Smith, Anton A. A.; Goldie, Kenneth N.; Zelikin, Alexander N.; Städler, Brigitte

2015-01-01

Recently, the combination of lipids and block copolymers has become an alternative to liposomes and polymersomes as nano-sized drug carriers. We synthesize novel block copolymers consisting of poly(cholesteryl acrylate) as the hydrophobic core and poly(N-isopropylacrylamide) (PNIPAAm) as the hydrophilic extensions. Their successful phospholipid-assisted assembly into vesicles is demonstrated using the evaporation-hydration method. The preserved thermo-responsive property of the lipid-polymer hybrids is shown by a temperature dependent adsorption behaviour of the vesicles to poly(l lysine) coated surfaces. As expected, the vesicle adsorption is found to be higher at elevated temperatures. The cellular uptake efficiency of hybrids is assessed using macrophages with applied shear stress. The amount of adhering macrophages is affected by the time and level of applied shear stress. Further, it is found that shorter PNIPAAm extensions lead to higher uptake of the assemblies by the macrophages with applied shear stress. No inherent cytotoxicity is observed at the tested conditions. Taken together, this first example of responsive lipid-polymer hybrids, and their positive biological evaluation makes them promising nano-sized drug carrier candidates. PMID:26339330

Method and apparatus for advancing tethers

DOEpatents

Zollinger, W.T.

1998-06-02

A tether puller for advancing a tether through a channel may include a bellows assembly having a leading end fixedly attached to the tether at a first position and a trailing end fixedly attached to the tether at a second position so that the leading and trailing ends of the bellows assembly are located a substantially fixed distance apart. The bellows assembly includes a plurality of independently inflatable elements each of which may be separately inflated to an extended position and deflated to a retracted position. Each of the independently inflatable elements expands radially and axially upon inflation. An inflation system connected to the independently inflatable elements inflates and deflates selected ones of the independently inflatable elements to cause the bellows assembly to apply a tractive force to the tether and advance it in the channel. 9 figs.

Probe tip heating assembly

DOE Office of Scientific and Technical Information (OSTI.GOV)

Schmitz, Roger William; Oh, Yunje

A heating assembly configured for use in mechanical testing at a scale of microns or less. The heating assembly includes a probe tip assembly configured for coupling with a transducer of the mechanical testing system. The probe tip assembly includes a probe tip heater system having a heating element, a probe tip coupled with the probe tip heater system, and a heater socket assembly. The heater socket assembly, in one example, includes a yoke and a heater interface that form a socket within the heater socket assembly. The probe tip heater system, coupled with the probe tip, is slidably receivedmore » and clamped within the socket.« less

Self-Assembly at the Colloidal Scale

NASA Astrophysics Data System (ADS)

Zhong, Xiao

The existence of self-assembly, the phenomenon of spontaneous structural formation from building blocks, transcends many orders of magnitude, ranging from molecular to cosmic. It is arguably the most common, important, and complex question in science. This thesis aims for understanding a spectrum of self-assembly-self assembly at the colloidal scale. Of the whole spectrum of self-assembly, the colloidal scale is of particular interest and importance to researchers, for not only comprehensive tools for colloidal scale studies have been well established, but also the various promising applications colloidal self-assembly can facilitate. In this thesis, a high throughput technique-Polymer Pen Lithography (PPL) is modified and its potential for creating corrals for colloidal assembly is evaluated. Then two different approaches of assembling colloids are explored in depth. One of them is by using a phenomenon called dielectrophoresis (DEP) as driving force to manipulate colloidal nucleation and crystal growth. And the other takes advantage of the Pt-catalyzed H2O 2 redox reaction to drive micrometer-scaled, rod-shaped colloids to swim and assemble. Lastly, an optical method called Holographic Video Microscopy (HVM) is used to monitor and characterize "bad" self-assembly of proteins, that is their aggregations. The four studies discussed in this thesis represent advancements in the colloidal scale from different aspects. The PPL technique enriched the toolbox for colloidal self-assembly. The DEP driven colloidal nucleation and crystal growth shed light on deeper understanding the mechanism of crystallization. And the swimming and assembly of micro-scale rods leads to kinetics reminiscent of bacterial run-and-tumble motion. Finally, the HVM technique for monitoring and understanding protein aggregation could potentially lead to better quality assurance for therapeutic proteins and could be a powerful tool for assessing their shelf lives.

42 CFR 84.20 - Examination, inspection, and testing of complete respirator assemblies; fees.

Code of Federal Regulations, 2010 CFR

2010-10-01

... assemblies; fees. Except as provided in § 84.22, the following fees shall be charged by the Institute for the examination, inspection and testing of complete respirator assemblies: Self-contained breathing apparatus... respirator assemblies; fees. 84.20 Section 84.20 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH...

42 CFR 84.20 - Examination, inspection, and testing of complete respirator assemblies; fees.

Code of Federal Regulations, 2011 CFR

2011-10-01

... assemblies; fees. Except as provided in § 84.22, the following fees shall be charged by the Institute for the examination, inspection and testing of complete respirator assemblies: Self-contained breathing apparatus... respirator assemblies; fees. 84.20 Section 84.20 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH...

42 CFR 84.20 - Examination, inspection, and testing of complete respirator assemblies; fees.

Code of Federal Regulations, 2014 CFR

2014-10-01

... respirator assemblies; fees. 84.20 Section 84.20 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH... assemblies; fees. Except as provided in § 84.22, the following fees shall be charged by the Institute for the examination, inspection and testing of complete respirator assemblies: Self-contained breathing apparatus...

42 CFR 84.20 - Examination, inspection, and testing of complete respirator assemblies; fees.

Code of Federal Regulations, 2012 CFR

2012-10-01

... respirator assemblies; fees. 84.20 Section 84.20 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH... assemblies; fees. Except as provided in § 84.22, the following fees shall be charged by the Institute for the examination, inspection and testing of complete respirator assemblies: Self-contained breathing apparatus...

42 CFR 84.20 - Examination, inspection, and testing of complete respirator assemblies; fees.

Code of Federal Regulations, 2013 CFR

2013-10-01

... respirator assemblies; fees. 84.20 Section 84.20 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH... assemblies; fees. Except as provided in § 84.22, the following fees shall be charged by the Institute for the examination, inspection and testing of complete respirator assemblies: Self-contained breathing apparatus...

Proton triggered circularly polarized luminescence in orthogonal- and co-assemblies of chiral gelators with achiral perylene bisimide.

PubMed

Han, Dongxue; Han, Jianlei; Huo, Shengwei; Qu, Zuoming; Jiao, Tifeng; Liu, Minghua; Duan, Pengfei

2018-05-29

The orthogonal- or co-assembly of achiral perylene bisimide (PBI) with chiral gelators can be regulated by solvents. While the coassembly leads to the formation of chiroptical nanofibers through chirality transfer, the orthogonal assemblies could not. Moreover, protonation on the coassembled nanofibers could light up the circularly polarized luminescence (CPL).

Lunar surface construction and assembly equipment study: Lunar Base Systems Study (LBSS) task 5.3

NASA Technical Reports Server (NTRS)

1988-01-01

A set of construction and assembly tasks required on the lunar surface was developed, different concepts for equipment applicable to the tasks determined, and leading candidate systems identified for future conceptual design. Data on surface construction and assembly equipment systems are necessary to facilitate an integrated review of a complete lunar scenario.

Receptor clustering drives polarized assembly of ankyrin.

PubMed

Jefford, G; Dubreuil, R R

2000-09-08

Expression of the L1 family cell adhesion molecule neuroglian in Drosophila S2 cells leads to cell aggregation and polarized ankyrin accumulation at sites of cell-cell contact. Thus neuroglian adhesion generates a spatial cue for polarized assembly of ankyrin and the spectrin cytoskeleton. Here we characterized a chimera of the extracellular and transmembrane domains of rat CD2 fused to the cytoplasmic domain of neuroglian. The chimera was used to test the hypothesis that clustering of neuroglian at sites of adhesion generates the signal that activates ankyrin binding. Abundant expression of the chimera at the plasma membrane was not a sufficient cue to drive ankyrin assembly, since ankyrin remained diffusely distributed throughout the cytoplasm of CD2-neuroglian-expressing cells. However, ankyrin became highly enriched at sites of antibody-induced capping of CD2-neuroglian. Spectrin codistributed with ankyrin at capped sites. A green fluorescent protein-tagged ankyrin was used to monitor ankyrin distribution in living cells. Enhanced green fluorescent protein-ankyrin behaved identically to antibody-stained endogenous ankyrin, proving that the polarized accumulation of ankyrin was not an artifact of fixing and staining cells. We propose a model in which clustering of neuroglian induces a conformational change in the cytoplasmic domain that drives polarized assembly of the spectrin cytoskeleton.

SEASAT study documentation

NASA Technical Reports Server (NTRS)

1974-01-01

The proposed spacecraft consists of a bus module, containing all subsystems required for support of the sensors, and a payload module containing all of the sensor equipment. The two modules are bolted together to form the spacecraft, and electrical interfaces are accomplished via mated connectors at the interface plane. This approach permits independent parallel assembly and test operations on each module up until mating for final spacecraft integration and test operations. Proposed program schedules recognize the need to refine sensor/spacecraft interfaces prior to proceeding with procurement, reflect the lead times estimated by suppliers for delivery of equipment, reflect a comprehensive test program, and provide flexibility for unanticipated problems. The spacecraft systems are described in detail along with aerospace ground equipment, ground handling equipment, the launch vehicle, imaging radar incorporation, and systems tests.

Internally damped, self-arresting vertical drop-weight impact test apparatus

NASA Technical Reports Server (NTRS)

Ambur, Damodar R. (Inventor); Prasad, Chunchu B. (Inventor); Waters, Jr., William A. (Inventor); Stockum, Robert W. (Inventor); Walter, Manfred A. (Inventor)

1996-01-01

A vertical dropped-weight impact test machine has a dropped-weight barrel vertically supported on upper and lower support brackets. The dropped-weight barrel is chambered to receive a dropped-weight assembly having a latch pin at its upper end, a damping unit in the middle, and a tup at its lower end. The tup is adapted for gathering data during impact testing. The latch pin releasably engages a latch pin coupling assembly. The latch pin coupling assembly is attached to a winch via a halyard for raising and lowering the dropped-weight assembly. The lower end of the dropped-weight barrel is provided with a bounce-back arresting mechanism which is activated by the descending passage of the dropped-weight assembly. After striking the specimen, the dropped-weight assembly rebounds vertically and is caught by the bounce-back arresting mechanism. The damping unit of the dropped-weight assembly serves to dissipate energy from the rebounding dropped-weight assembly and prevents the dropped-weight assembly from rebounding from the self-arresting mechanism.

Internally damped, self-arresting vertical drop-weight impact test apparatus

NASA Technical Reports Server (NTRS)

Ambur, Damodar R. (Inventor); Prasad, Chunchu B. (Inventor); Waters, Jr., William A. (Inventor); Stockum, Robert W. (Inventor); Water, Manfred A. (Inventor)

1995-01-01

A vertical dropped-weight impact test machine has a dropped-weight barrel vertically supported on upper and lower support brackets. The dropped-weight barrel is chambered to receive a dropped-weight assembly having a latch pin at its upper end, a damping unit in the middle, and a tup at its lower end. The tup is adapted for gathering data during impact testing. The latch pin releasably engages a latch pin coupling assembly. The latch pin coupling assembly is attached to a winch via a halyard for raising and lowering the dropped-weight assembly. The lower end of the dropped-weight barrel is provided with a bounce-back arresting mechanism which is activated by the descending passage of the dropped-weight assembly. After striking the specimen, the dropped-weight assembly rebounds vertically and is caught by the bounce-back arresting mechanism. The damping unit of the dropped-weight assembly serves to dissipate energy from the rebounding dropped-weight assembly and prevents the dropped-weight assembly from rebounding from the self-arresting mechanism.

Robotic space construction

NASA Technical Reports Server (NTRS)

Mixon, Randolph W.; Hankins, Walter W., III; Wise, Marion A.

1988-01-01

Research at Langley AFB concerning automated space assembly is reviewed, including a Space Shuttle experiment to test astronaut ability to assemble a repetitive truss structure, testing the use of teleoperated manipulators to construct the Assembly Concept for Construction of Erectable Space Structures I truss, and assessment of the basic characteristics of manipulator assembly operations. Other research topics include the simultaneous coordinated control of dual-arm manipulators and the automated assembly of candidate Space Station trusses. Consideration is given to the construction of an Automated Space Assembly Laboratory to study and develop the algorithms, procedures, special purpose hardware, and processes needed for automated truss assembly.

Testing the Large-scale Environments of Cool-core and Non-cool-core Clusters with Clustering Bias

NASA Astrophysics Data System (ADS)

Medezinski, Elinor; Battaglia, Nicholas; Coupon, Jean; Cen, Renyue; Gaspari, Massimo; Strauss, Michael A.; Spergel, David N.

2017-02-01

There are well-observed differences between cool-core (CC) and non-cool-core (NCC) clusters, but the origin of this distinction is still largely unknown. Competing theories can be divided into internal (inside-out), in which internal physical processes transform or maintain the NCC phase, and external (outside-in), in which the cluster type is determined by its initial conditions, which in turn leads to different formation histories (I.e., assembly bias). We propose a new method that uses the relative assembly bias of CC to NCC clusters, as determined via the two-point cluster-galaxy cross-correlation function (CCF), to test whether formation history plays a role in determining their nature. We apply our method to 48 ACCEPT clusters, which have well resolved central entropies, and cross-correlate with the SDSS-III/BOSS LOWZ galaxy catalog. We find that the relative bias of NCC over CC clusters is b = 1.42 ± 0.35 (1.6σ different from unity). Our measurement is limited by the small number of clusters with core entropy information within the BOSS footprint, 14 CC and 34 NCC clusters. Future compilations of X-ray cluster samples, combined with deep all-sky redshift surveys, will be able to better constrain the relative assembly bias of CC and NCC clusters and determine the origin of the bimodality.

Testing the Large-scale Environments of Cool-core and Non-cool-core Clusters with Clustering Bias

DOE Office of Scientific and Technical Information (OSTI.GOV)

Medezinski, Elinor; Battaglia, Nicholas; Cen, Renyue

2017-02-10

There are well-observed differences between cool-core (CC) and non-cool-core (NCC) clusters, but the origin of this distinction is still largely unknown. Competing theories can be divided into internal (inside-out), in which internal physical processes transform or maintain the NCC phase, and external (outside-in), in which the cluster type is determined by its initial conditions, which in turn leads to different formation histories (i.e., assembly bias). We propose a new method that uses the relative assembly bias of CC to NCC clusters, as determined via the two-point cluster-galaxy cross-correlation function (CCF), to test whether formation history plays a role in determiningmore » their nature. We apply our method to 48 ACCEPT clusters, which have well resolved central entropies, and cross-correlate with the SDSS-III/BOSS LOWZ galaxy catalog. We find that the relative bias of NCC over CC clusters is b = 1.42 ± 0.35 (1.6 σ different from unity). Our measurement is limited by the small number of clusters with core entropy information within the BOSS footprint, 14 CC and 34 NCC clusters. Future compilations of X-ray cluster samples, combined with deep all-sky redshift surveys, will be able to better constrain the relative assembly bias of CC and NCC clusters and determine the origin of the bimodality.« less

iLIDS Simulations and Videos for Docking TIM

NASA Technical Reports Server (NTRS)

Lewis, James L.

2010-01-01

The video shows various aspects of the International Low Impact Docking System, including team members, some production, configuration, mated androgynous iLIDS, SCS Lockdown system, thermal analysis, electrical engineering aspects, the iLIDS control box and emulator, radiation testing at BNL, component environmental testing, component vibration testing, 3G processor board delivery system, GTA vibe test, EMA testbed, hook and hook disassembly, flex shaftdrive assembly, GSE cradle MISSE-6 Columbus, MISSE 6 and 7 seal experiments, actuated full scale seal test rig, LIDS on Hubble, dynamics test prep, EDU 54 mass emulation and SCS, load ring characterization, 6DOF proof test, SCS at 6DOF, machining EEMS and inner ring assembly, APAS assembly, inner ring fitting, rotation stand assembly, EEMS mating, and EEMS proof of concept demonstration.

Design of a cryogenic system for a 20m direct current superconducting MgB2 and YBCO power cable

NASA Astrophysics Data System (ADS)

Cheadle, Michael J.; Bromberg, Leslie; Jiang, Xiaohua; Glowacki, Bartek; Zeng, Rong; Minervini, Joseph; Brisson, John

2014-01-01

The Massachusetts Institute of Technology, the University of Cambridge in the United Kingdom, and Tsinghua University in Beijing, China, are collaborating to design, construct, and test a 20 m, direct current, superconducting MgB2 and YBCO power cable. The cable will be installed in the State Key Laboratory of Power Systems at Tsinghua University in Beijing beginning in 2013. In a previous paper [1], the cryogenic system was briefly discussed, focusing on the cryogenic issues for the superconducting cable. The current paper provides a detailed discussion of the design, construction, and assembly of the cryogenic system and its components. The two-stage system operates at nominally 80 K and 20 K with the primary cryogen being helium gas. The secondary cryogen, liquid nitrogen, is used to cool the warm stage of binary current leads. The helium gas provides cooling to both warm and cold stages of the rigid cryostat housing the MgB2 and YBCO conductors, as well as the terminations of the superconductors at the end of the current leads. A single cryofan drives the helium gas in both stages, which are thermally isolated with a high effectiveness recuperator. Refrigeration for the helium circuit is provided by a Sumitomo RDK415 cryocooler. This paper focuses on the design, construction, and assembly of the cryostat, the recuperator, and the current leads with associated superconducting cable terminations.

Cryogenic Optical Performance of a Light-weight Mirror Assembly for Future Space Astronomical Telescopes: Optical Test Results and Thermal Optical Model

NASA Technical Reports Server (NTRS)

Eng, Ron; Arnold, William; Baker, Markus A.; Bevan, Ryan M.; Carpenter, James R.; Effinger, Michael R.; Gaddy, Darrell E.; Goode, Brian K.; Kegley, Jeffrey R.; Hogue, William D.;

Functional Redundancy in HIV-1 Viral Particle Assembly

PubMed Central

O'Carroll, Ina P.; Crist, Rachael M.; Mirro, Jane; Harvin, Demetria; Soheilian, Ferri; Kamata, Anne; Nagashima, Kunio

2012-01-01

Expression of a retroviral Gag protein in mammalian cells leads to the assembly of virus particles. In vitro, recombinant Gag proteins are soluble but assemble into virus-like particles (VLPs) upon addition of nucleic acid. We have proposed that Gag undergoes a conformational change when it is at a high local concentration and that this change is an essential prerequisite for particle assembly; perhaps one way that this condition can be fulfilled is by the cooperative binding of Gag molecules to nucleic acid. We have now characterized the assembly in human cells of HIV-1 Gag molecules with a variety of defects, including (i) inability to bind to the plasma membrane, (ii) near-total inability of their capsid domains to engage in dimeric interaction, and (iii) drastically compromised ability to bind RNA. We find that Gag molecules with any one of these defects still retain some ability to assemble into roughly spherical objects with roughly correct radius of curvature. However, combination of any two of the defects completely destroys this capability. The results suggest that these three functions are somewhat redundant with respect to their contribution to particle assembly. We suggest that they are alternative mechanisms for the initial concentration of Gag molecules; under our experimental conditions, any two of the three is sufficient to lead to some semblance of correct assembly. PMID:22993163

Assembly Test Article (ATA)

NASA Technical Reports Server (NTRS)

Ricks, Glen A.

1988-01-01

The assembly test article (ATA) consisted of two live loaded redesigned solid rocket motor (RSRM) segments which were assembled and disassembled to simulate the actual flight segment stacking process. The test assembly joint was flight RSRM design, which included the J-joint insulation design and metal capture feature. The ATA test was performed mid-November through 24 December 1987, at Kennedy Space Center (KSC), Florida. The purpose of the test was: certification that vertical RSRM segment mating and separation could be accomplished without any damage; verification and modification of the procedures in the segment stacking/destacking documents; and certification of various GSE to be used for flight assembly and inspection. The RSRM vertical segment assembly/disassembly is possible without any damage to the insulation, metal parts, or seals. The insulation J-joint contact area was very close to the predicted values. Numerous deviations and changes to the planning documents were made to ensure the flight segments are effectively and correctly stacked. Various GSE were also certified for use on flight segments, and are discussed in detail.

Characterization of the Twelve Channel 100/140 Micron Optical Fiber, Ribbon Cable and MTP Array Connector Assembly for Space Flight Environments

NASA Technical Reports Server (NTRS)

Ott, Melanie N.; Macmurphy, Shawn; Friedberg, Patricia; Day, John H. (Technical Monitor)

2002-01-01

Presented here is the second set of testing conducted by the Technology Validation Laboratory for Photonics at NASA Goddard Space Flight Center on the 12 optical fiber ribbon cable with MTP array connector for space flight environments. In the first set of testing the commercial 62.5/125 cable assembly was characterized using space flight parameters. The testing showed that the cable assembly would survive a typical space flight mission with the exception of a vacuum environment. Two enhancements were conducted to the existing technology to better suit the vacuum environment as well as the existing optoelectronics and increase the reliability of the assembly during vibration. The MTP assembly characterized here has a 100/140 optical commercial fiber and non outgassing connector and cable components. The characterization for this enhanced fiber optic cable assembly involved vibration, thermal and radiation testing. The data and results of this characterization study are presented which include optical in-situ testing.

Final Assembly and Factory Testing of the Jefferson Lab SHMS Spectrometer Quadrupole and Dipole Superconducting Magnets

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brindza, Paul; Lassiter, Steven; Sun, Eric

Jefferson Lab is constructing an 11 Gev/c electron spectrometer called the Super High Momentum Spectrometer (SHMS) as part of the 12 GeV JLAB upgrade for experimental Hall C. Three of the five superconducting(SC) SHMS magnets are under construction at SigmaPhi in Vannes France as a result of an international competition for design and fabrication. The three magnets Q2 and Q3 60 cm bore quadrupoles and the 60 cm warm bore dipole are complete or near complete and have many design features in common. All three magnets share a common superconductor, collaring system, cryostat design, cold to warm support, cryogenic interface,more » burnout resistant current leads, DC power supply, quench protection, instrumentation and controls. The three magnets are collared, installed in cryostats and welded up and in various stages of final testing. The Q2 quadrupole is due to ship from France to America in August arriving during this ASC conference and has passed all final hipot, leak and pressure tests. The dipole is in leak and pressure testing as of July 2016 while the Q3 quadrupole requires some outer vacuum vessel assembly. Delivery of the Q3 and Dipole magnets will follow the Q2 at about 1 month intervals. Lastly, factory testing have included hipot and electrical tests, magnetic tests at low field, mechanical alignments to center the coils, leak tests and ASME Code required pressure tests. Upon installation in Hall C at JLAB cold testing will commence.« less

Final Assembly and Factory Testing of the Jefferson Lab SHMS Spectrometer Quadrupole and Dipole Superconducting Magnets

DOE PAGES

Brindza, Paul; Lassiter, Steven; Sun, Eric; ...

2017-06-01

Jefferson Lab is constructing an 11 Gev/c electron spectrometer called the Super High Momentum Spectrometer (SHMS) as part of the 12 GeV JLAB upgrade for experimental Hall C. Three of the five superconducting(SC) SHMS magnets are under construction at SigmaPhi in Vannes France as a result of an international competition for design and fabrication. The three magnets Q2 and Q3 60 cm bore quadrupoles and the 60 cm warm bore dipole are complete or near complete and have many design features in common. All three magnets share a common superconductor, collaring system, cryostat design, cold to warm support, cryogenic interface,more » burnout resistant current leads, DC power supply, quench protection, instrumentation and controls. The three magnets are collared, installed in cryostats and welded up and in various stages of final testing. The Q2 quadrupole is due to ship from France to America in August arriving during this ASC conference and has passed all final hipot, leak and pressure tests. The dipole is in leak and pressure testing as of July 2016 while the Q3 quadrupole requires some outer vacuum vessel assembly. Delivery of the Q3 and Dipole magnets will follow the Q2 at about 1 month intervals. Lastly, factory testing have included hipot and electrical tests, magnetic tests at low field, mechanical alignments to center the coils, leak tests and ASME Code required pressure tests. Upon installation in Hall C at JLAB cold testing will commence.« less

Aerodynamic characteristics of a propeller-powered high-lift semispan wing

NASA Technical Reports Server (NTRS)

Gentry, Garl L., Jr.; Takallu, M. A.; Applin, Zachary T.

1994-01-01

A small-scale semispan high-lift wing-flap system equipped under the wing with a turboprop engine assembly was tested in the LaRC 14- by 22-Foot Subsonic Tunnel. Experimental data were obtained for various propeller rotational speeds, nacelle locations, and nacelle inclinations. To isolate the effects of the high lift system, data were obtained with and without the flaps and leading-edge device. The effects of the propeller slipstream on the overall longitudinal aerodynamic characteristics of the wing-propeller assembly were examined. Test results indicated that the lift coefficient of the wing could be increased by the propeller slipstream when the rotational speed was increased and high-lift devices were deployed. Decreasing the nacelle inclination (increased pitch down) enhanced the lift performance of the system much more than varying the vertical or horizontal location of the nacelle. Furthermore, decreasing the nacelle inclination led to higher lift curve slope values, which indicated that the powered wing could sustain higher angles of attack near maximum lift performance. Any lift augmentation was accompanied by a drag penalty due to the increased wing lift.

76 FR 19468 - Amended Certification Regarding Eligibility To Apply for Worker Adjustment Assistance

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-07

... Known As ATW Automation, Inc., Livonia Michigan TA-W-72,075A Assembly & Test Worldwide, Inc., Currently... Saginaw, Michigan locations of Assembly & Test Worldwide, Inc., are currently known as ATW Automation, Inc... Automation, Inc., Livonia, Michigan (TA-W-72,075); Assembly & Test Worldwide, Inc., currently known as ATW...

Using Set Covering with Item Sampling to Analyze the Infeasibility of Linear Programming Test Assembly Models

ERIC Educational Resources Information Center

Huitzing, Hiddo A.

2004-01-01

This article shows how set covering with item sampling (SCIS) methods can be used in the analysis and preanalysis of linear programming models for test assembly (LPTA). LPTA models can construct tests, fulfilling a set of constraints set by the test assembler. Sometimes, no solution to the LPTA model exists. The model is then said to be…

Coaxial test fixture

DOEpatents

Praeg, Walter F.

1986-01-01

An assembly is provided for testing one or more contact material samples in a vacuum environment. The samples are positioned as an inner conductive cylinder assembly which is mounted for reciprocal vertical motion as well as deflection from a vertical axis. An outer conductive cylinder is coaxially positioned around the inner cylinder and test specimen to provide a vacuum enclosure therefor. A power source needed to drive test currents through the test specimens is connected to the bottom of each conductive cylinder, through two specially formed conductive plates. The plates are similar in form, having a plurality of equal resistance current paths connecting the power source to a central connecting ring. The connecting rings are secured to the bottom of the inner conductive assembly and the outer cylinder, respectively. A hydraulic actuator is also connected to the bottom of the inner conductor assembly to adjust the pressure applied to the test specimens during testing. The test assembly controls magnetic forces such that the current distribution through the test samples is symmetrical and that contact pressure is not reduced or otherwise disturbed.

X-ray structural analysis of two-dimensional assembling lead sulfide nanocrystals of different sizes

NASA Astrophysics Data System (ADS)

Ushakova, Elena V.; Golubkov, Valery V.; Litvin, Aleksandr P.; Parfenov, Peter S.; Cherevkov, Sergei A.; Fedorov, Anatoly V.; Baranov, Alexander V.

2016-08-01

We report on the structural investigation of self-organized assemblies of PbS nanocrystals (NCs) of different sizes, which were deposited on a glass substrate or embedded in a porous matrix. Regardless of the NC size and the type of the substrate and matrix, the assemblies were ordered in two-dimensional superlattices with densely packed NCs.

Thermocouple assembly

DOEpatents

Thermos, Anthony Constantine; Rahal, Fadi Elias

2002-01-01

A thermocouple assembly includes a thermocouple; a plurality of lead wires extending from the thermocouple; an insulating jacket extending along and enclosing the plurality of leads; and at least one internally sealed area within the insulating jacket to prevent fluid leakage along and within the insulating jacket. The invention also provides a method of preventing leakage of a fluid along and through an insulating jacket of a thermocouple including the steps of a) attaching a plurality of lead wires to a thermocouple; b) adding a heat sensitive pseudo-wire to extend along the plurality of lead wires; c) enclosing the lead wires and pseudo-wire inside an insulating jacket; d) locally heating axially spaced portions of the insulating jacket to a temperature which melts the pseudo-wire and fuses it with an interior surface of the jacket.

Analysis of lead free tin-silver-copper and tin-lead solder wetting reactions

NASA Astrophysics Data System (ADS)

Anson, Scott J.

Lead free electronics soldering is driven by a combination of health and environmental concerns, international legislation and marketing pressure by lead free electronics manufacturing competitors. Since July 1, 2006, companies that do not comply with the European Union legislation are not able to sell circuit assemblies with lead solder in the European Union. China has developed its own regulations, based on the European Union documents with a compliance date of March 1, 2007. Extensive testing by the electronics community has determined that the Sn - Ag - Cu (SAC) family of alloys is the preferred choice for lead free Surface Mount Technology (SMT) soldering. The 96.5Sn/3.0Ag/0.5Cu alloy was used in this study. Lead free soldering requires an increase in reflow peak temperatures which further aggravates component moisture sensitivity risks and thereby decreases assembly yield. Prior research has revealed an enhanced solder spreading phenomena at lower peak temperature and shorter time above liquidus with 63Sn/37Pb solder. This current research investigated solder wetting reactions in 63Sn/37Pb and 96.5Sn/3.0Ag/0.5Cu (SAC305) using materials and manufacturing systems that are industry relevant. The objective was to advance the knowledge base of metal wetting while developing a reflow assembly process that minimized the component defect rates. The components are damaged during reflow by popcorn delamination, which is the result of moisture absorption and subsequent rapid evaporation. A classical Design Of Experiments (DOE) approach was used, with wetted area as the response variable. Outside of the DOE, substrate dissolution depth, and substrate surface new phase formation (reaction product) distance from the triple line (solder wetting front) and reaction product thickness in the solder joint (under the solder) were also analyzed. The samples were analyzed for correlation of reflow peak temperature, reflow Time Above Liquidus (TAL), wetted area, reaction product distance from the triple line, substrate dissolution depth, triple line ridge (substrate protrusion into the molten solder) formation and reaction product thickness in the solder joint. The general results are (1) an improved understanding of 63Sn/37Pb and 96.5Sn/3.0Ag/0.5Cu WT% solder wetting reactions, (2) reduced 63Sn/37Pb and SAC reflow peak temperatures, and thereby reduced risk of moisture sensitivity damage to components. The significance of these results are (1) enhanced applied understanding of the complexity of molten metal wetting a substrate and (2) enhanced assembly yield due to minimal aggravation of component moisture sensitivity. The uniqueness of this research is that it utilized a holistic Systems Science approach which provided a combined microscopic (substrate and molten metal reactions) and macroscopic (wetted area) analysis of metal wetting using materials and processes that were directly relevant to electronics manufacturing.

Design, manufacture, and test of coolant pump-motor assembly for Brayton power conversion system

NASA Technical Reports Server (NTRS)

Gabacz, L. E.

1973-01-01

The design, development, fabrication, and testing of seven coolant circulating pump-motor assemblies are discussed. The pump-motor assembly is driven by the nominal 44.4-volt, 400-Hz, 3-phase output of a nominal 56-volt dc input inverter. The pump-motor assembly will be used to circulate Dow Corning 200 liquid coolant for use in a Brayton cycle space power system. The pump-motor assembly develops a nominal head of 70 psi at 3.7 gpm with an over-all efficiency of 26 percent. The design description, drawings, photographs, reliability results, and developmental and acceptance test results are included.

Advanced Stirling Radioisotope Generator Engineering Unit 2 (ASRG EU2) Final Assembly

NASA Technical Reports Server (NTRS)

Oriti, Salvatore M.

2015-01-01

NASA Glenn Research Center (GRC) has recently completed the assembly of a unique Stirling generator test article for laboratory experimentation. Under the Advanced Stirling Radioisotope Generator (ASRG) flight development contract, NASA GRC initiated a task to design and fabricate a flight-like generator for in-house testing. This test article was given the name ASRG Engineering Unit 2 (EU2) as it was effectively the second engineering unit to be built within the ASRG project. The intent of the test article was to duplicate Lockheed Martin's qualification unit ASRG design as much as possible to enable system-level tests not previously possible at GRC. After the cancellation of the ASRG flight development project, the decision was made to continue the EU2 build, and make use of a portion of the hardware from the flight development project. GRC and Lockheed Martin engineers collaborated to develop assembly procedures, leveraging the valuable knowledge gathered by Lockheed Martin during the ASRG development contract. The ASRG EU2 was then assembled per these procedures at GRC with Lockheed Martin engineers on site. The assembly was completed in August 2014. This paper details the components that were used for the assembly, and the assembly process itself.

16 CFR Figure 1 to Part 1633 - Test Assembly, Shown in Furniture Calorimeter (Configuration A)

Code of Federal Regulations, 2014 CFR

2014-01-01

... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Test Assembly, Shown in Furniture Calorimeter (Configuration A) 1 Figure 1 to Part 1633 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION.... 1 Figure 1 to Part 1633—Test Assembly, Shown in Furniture Calorimeter (Configuration A) ER15MR06.000 ...

16 CFR Figure 1 to Part 1633 - Test Assembly, Shown in Furniture Calorimeter (Configuration A)

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Test Assembly, Shown in Furniture Calorimeter (Configuration A) 1 Figure 1 to Part 1633 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION.... 1 Figure 1 to Part 1633—Test Assembly, Shown in Furniture Calorimeter (Configuration A) ER15MR06.000 ...

16 CFR Figure 1 to Part 1633 - Test Assembly, Shown in Furniture Calorimeter (Configuration A)

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Test Assembly, Shown in Furniture Calorimeter (Configuration A) 1 Figure 1 to Part 1633 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION.... 1 Figure 1 to Part 1633—Test Assembly, Shown in Furniture Calorimeter (Configuration A) ER15MR06.000 ...

16 CFR Figure 1 to Part 1633 - Test Assembly, Shown in Furniture Calorimeter (Configuration A)

Code of Federal Regulations, 2012 CFR

2012-01-01

... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Test Assembly, Shown in Furniture Calorimeter (Configuration A) 1 Figure 1 to Part 1633 Commercial Practices CONSUMER PRODUCT SAFETY COMMISSION.... 1 Figure 1 to Part 1633—Test Assembly, Shown in Furniture Calorimeter (Configuration A) ER15MR06.000 ...

Heat release from wood wall assemblies using oxygen consumption method

Treesearch

Hao C. Tran; Robert E. White

1990-01-01

The concept of heat release rate is gaining acceptance in the evaluation of fire performance of materials and assemblies. However, this concept has not been incorporated into fire endurance testing such as the ASTM E-119 test method. Heat release rate of assemblies can be useful in determining the time at which the assemblies start to contribute to the controlled fire...

Mobile work station concept for assembly of large space structures (zero gravity simulation tests)

NASA Astrophysics Data System (ADS)

Heard, W. L., Jr.; Bush, H. G.; Wallsom, R. E.; Jensen, J. K.

1982-03-01

The concept presented is intended to enhance astronaut assembly of truss structure that is either too large or complex to fold for efficient Shuttle delivery to orbit. The potential of augmented astronaut assembly is illustrated by applying the result of the tests to a barebones assembly of a truss structure. If this structure were assembled from the same nestable struts that were used in the Mobile Work Station assembly tests, the spacecraft would be 55 meters in diameter and consist of about 500 struts. The struts could be packaged in less than 1/2% of the Shuttle cargo bay volume and would take up approximately 3% of the mass lift capability. They could be assembled in approximately four hours. This assembly concept for erectable structures is not only feasible, but could be used to significant economic advantage by permitting the superior packaging feature of erectable structures to be exploited and thereby reduce expensive Shuttle delivery flights.

Mobile work station concept for assembly of large space structures (zero gravity simulation tests)

NASA Technical Reports Server (NTRS)

Heard, W. L., Jr.; Bush, H. G.; Wallsom, R. E.; Jensen, J. K.

1982-01-01

The concept presented is intended to enhance astronaut assembly of truss structure that is either too large or complex to fold for efficient Shuttle delivery to orbit. The potential of augmented astronaut assembly is illustrated by applying the result of the tests to a barebones assembly of a truss structure. If this structure were assembled from the same nestable struts that were used in the Mobile Work Station assembly tests, the spacecraft would be 55 meters in diameter and consist of about 500 struts. The struts could be packaged in less than 1/2% of the Shuttle cargo bay volume and would take up approximately 3% of the mass lift capability. They could be assembled in approximately four hours. This assembly concept for erectable structures is not only feasible, but could be used to significant economic advantage by permitting the superior packaging feature of erectable structures to be exploited and thereby reduce expensive Shuttle delivery flights.

Consumption of lead-shot cervid meat and blood lead concentrations in a group of adult Norwegians.

PubMed

Meltzer, H M; Dahl, H; Brantsæter, A L; Birgisdottir, B E; Knutsen, H K; Bernhoft, A; Oftedal, B; Lande, U S; Alexander, J; Haugen, M; Ydersbond, T A

2013-11-01

Several recent investigations have reported high concentrations of lead in samples of minced cervid meat. This paper describes findings from a Norwegian study performed in 2012 among 147 adults with a wide range of cervid game consumption. The main aim was to assess whether high consumption of lead-shot cervid meat is associated with increased concentration of lead in blood. A second aim was to investigate to what extent factors apart from game consumption explain observed variability in blood lead levels. Median (5 and 95 percentile) blood concentration of lead was 16.6 µg/L (7.5 and 39 µg/L). An optimal multivariate linear regression model for log-transformed blood lead indicated that cervid game meat consumption once a month or more was associated with approximately 31% increase in blood lead concentrations. The increase seemed to be mostly associated with consumption of minced cervid meat, particularly purchased minced meat. However, many participants with high and long-lasting game meat intake had low blood lead concentrations. Cervid meat together with number of bullet shots per year, years with game consumption, self-assembly of bullets, wine consumption and smoking jointly accounted for approximately 25% of the variation in blood lead concentrations, while age and sex accounted for 27% of the variance. Blood lead concentrations increased approximately 18% per decade of age, and men had on average 30% higher blood lead concentrations than women. Hunters who assembled their own ammunition had 52% higher blood lead concentrations than persons not making ammunition. In conjunction with minced cervid meat, wine intake was significantly associated with increased blood lead. Our results indicate that hunting practices such as use of lead-based ammunition, self-assembling of lead containing bullets and inclusion of lead-contaminated meat for mincing to a large extent determine the exposure to lead from cervid game consumption. © 2013 Elsevier Inc. All rights reserved.

Comparative analysis of international standards for the fatigue testing of posterior spinal fixation systems: the importance of preload in ISO 12189.

PubMed

La Barbera, Luigi; Ottardi, Claudia; Villa, Tomaso

2015-10-01

Preclinical evaluation of the mechanical reliability of fixation devices is a mandatory activity before their introduction into market. There are two standardized protocols for preclinical testing of spinal implants. The American Society for Testing Materials (ASTM) recommends the F1717 standard, which describes a vertebrectomy condition that is relatively simple to implement, whereas the International Organization for Standardization (ISO) suggests the 12189 standard, which describes a more complex physiological anterior support-based setup. Moreover, ASTM F1717 is nowadays well established, whereas ISO 12189 has received little attention: A few studies tried to accurately describe the ISO experimental procedure through numeric models, but these studies totally neglect the recommended precompression step. This study aimed to build up a reliable, validated numeric model capable of describing the stress on the rods of a spinal fixator assembled according to ISO 12189 standard procedure. Such a model would more adequately represent the in vitro testing condition. This study used finite element (FE) simulations and experimental validation testing. An FE model of the ISO setup was built to calculate the stress on the rods. Simulation was validated by comparison with experimental strain gauges measurements. The same fixator has been previously virtually mounted in an L2-L4 FE model of the lumbar spine, and stresses in the rods were calculated when the spine was subjected to physiological forces and moments. The comparison between the FE predictions and experimental measurements is in good agreement, thus confirming the suitability of the FE method to evaluate the stresses in the device. The initial precompression induces a significant extension of the assembled construct. As the applied load increases, the initial extension is gradually compensated, so that at peak load the rods are bent in flexion: The final stress value predicted is thus reduced to about 50%, if compared with the previous model where the precompression was not considered. Neglecting the initial preload due to the assembly of the overall construct according to ISO 12189 standard could lead to an overestimation of the stress on the rods up to 50%. To correctly describe the state of stress on the posterior spinal fixator, tested according to the ISO procedure, it is important to take into account the initial preload due to the assembly of the overall construct. Copyright © 2015 Elsevier Inc. All rights reserved.

14. VIEW TO WESTSOUTHWEST ACROSS CAR TEST TRACT SITE TO ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

14. VIEW TO WEST-SOUTHWEST ACROSS CAR TEST TRACT SITE TO NORTH END OF ASSEMBLY PLANT, SHOWING NORTH END ADDITIONS. - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

A mobile work station concept for mechanically aided astronaut assembly of large space trusses

NASA Technical Reports Server (NTRS)

Heard, W. L., Jr.; Bush, H. G.; Wallson, R. E.; Jensen, J. K.

1983-01-01

This report presents results of a series of truss assembly tests conducted to evaluate a mobile work station concept intended to mechanically assist astronaut manual assembly of erectable space trusses. The tests involved assembly of a tetrahedral truss beam by a pair of test subjects with and without pressure (space) suits, both in Earth gravity and in simulated zero gravity (neutral buoyancy in water). The beam was assembled from 38 identical graphite-epoxy nestable struts, 5.4 m in length with aluminum quick-attachment structural joints. Struts and joints were designed to closely simulate flight hardware. The assembled beam was approximately 16.5 m long and 4.5 m on each of the four sides of its diamond-shaped cross section. The results show that average in-space assembly rates of approximately 38 seconds per strut can be expected for struts of comparable size. This result is virtually independent of the overall size of the structure being assembled. The mobile work station concept would improve astronaut efficiency for on-orbit manual assembly of truss structures, and also this assembly-line method is highly competitive with other construction methods being considered for large space structures.

Orbital Verification of the CXO High-Resolution Mirror Assembly Alignment and Vignetting

NASA Technical Reports Server (NTRS)

Gaetz, T. J.; Jerius, D.; Edgar, R. J.; VanSpeybroeck, L. P.; Schwartz, D. A.; Markevitch, M.; Schulz, N. S.

2000-01-01

Prior to launch, the High Resolution Mirror Assembly (HRMA) of the Chandra X-ray Observatory underwent extensive ground testing at the X-ray Calibration Facility (XRCF) at the Marshall Space Flight Center in Huntsville. Observations made during the post-launch Orbital Activation and Calibration period, allow the on-orbit condition of the X-ray optics to be assessed. Based on these ground-based and on-orbit data, we examine the alignment of the x-ray optics based on the PSF, and the boresight and alignment of the optical axis alignment relative to the detectors. We examine the vignetting and the single reflection ghost suppression properties of the telescope. Slight imperfections in alignment lead to a small azimuthal dependence of the off-axis area; the morphology of off-axis images also shows an additional small azimuthal dependence varying as 1/2 the off-axis azimuth angle.

Humidity Data for 9975 Shipping Packages with Softwood Fiberboard

DOE Office of Scientific and Technical Information (OSTI.GOV)

Daugherty, W. L.

The 9975 surveillance program is developing a technical basis to support extending the storage period of 9975 packages in K-Area Complex beyond the currently approved 15 years. A key element of this effort is developing a better understanding of degradation of the fiberboard assembly under storage conditions. This degradation is influenced greatly by the moisture content of the fiberboard, which is not well characterized on an individual package basis. Direct measurements of humidity and fiberboard moisture content have been made on two test packages with softwood fiberboard and varying internal heat levels from 0 up to 19W. Comparable measurements withmore » cane fiberboard have been reported previously. With an internal heat load, a temperature gradient in the fiberboard assembly leads to varying relative humidity in the air around the fiberboard. However, the absolute humidity tends to remain approximately constant throughout the package, especially at lower heat loads.« less

Induced-Dipole-Directed, Cooperative Self-Assembly of a Benzotrithiophene.

PubMed

Ikeda, Toshiaki; Adachi, Hiroaki; Fueno, Hiroyuki; Tanaka, Kazuyoshi; Haino, Takeharu

2017-10-06

A benzotrithiophene derivative possessing phenylisoxazoles self-assembled to form stacks. The molecule isodesmically self-assembled in chloroform, whereas it self-assembled in a cooperative fashion in decalin and in methylcyclohexane. Thermodynamic studies based on isodesmic, van der Schoot, and Goldstein-Stryer mathematical models revealed that the self-assembly processes are enthalpically driven and entropically opposed. An enthalpy-entropy compensation plot indicates that the assembly processes in chloroform, decalin, and methylcyclohexane are closely related. The enthalpic gains in less-polar solvents are greater than those in more-polar solvents, resulting in the formation of large assemblies in decalin and in methylcyclohexane. The formation of large assemblies leads to cooperative assemblies. The elongation process is enthalpically more favored than the nucleation process, which drives the cooperativity of the self-assembly. DFT calculations suggested that a hexameric assembly is more stable than tetrameric or dimeric assemblies. Cooperative self-assemblies based on intermolecular interactions other than hydrogen bonding have rarely been reported. It is demonstrated herein that van der Waals interactions, including induced dipole-dipole interactions, can drive the cooperative assembly of planar π-conjugated molecules.

Self-Assembly of Octopus Nanoparticles into Pre-Programmed Finite Clusters

NASA Astrophysics Data System (ADS)

Halverson, Jonathan; Tkachenko, Alexei

2012-02-01

The precise control of the spatial arrangement of nanoparticles (NP) is often required to take full advantage of their novel optical and electronic properties. NPs have been shown to self-assemble into crystalline structures using either patchy surface regions or complementary DNA strands to direct the assembly. Due to a lack of specificity of the interactions these methods lead to only a limited number of structures. An emerging approach is to bind ssDNA at specific sites on the particle surface making so-called octopus NPs. Using octopus NPs we investigate the inverse problem of the self-assembly of finite clusters. That is, for a given target cluster (e.g., arranging the NPs on the vertices of a dodecahedron) what are the minimum number of complementary DNA strands needed for the robust self-assembly of the cluster from an initially homogeneous NP solution? Based on the results of Brownian dynamics simulations we have compiled a set of design rules for various target clusters including cubes, pyramids, dodecahedrons and truncated icosahedrons. Our approach leads to control over the kinetic pathway and has demonstrated nearly perfect yield of the target.

Gasoline-Engine Assembler (engine & turbine) 806.781; Internal-Combustion-Engine-Assembler (engine & turbine) 806.781; Outboard-Motor Assembler (engine & turbine) 806.781--Technical Report on Development of USTES Aptitude Test Battery.

ERIC Educational Resources Information Center

Manpower Administration (DOL), Washington, DC. U.S. Training and Employment Service.

The United States Training and Employment Service General Aptitude Test Battery (GATB), first published in 1947, has been included in a continuing program of research to validate the tests against success in many different occupations. The GATB consists of 12 tests which measure nine aptitudes: General Learning Ability; Verbal Aptitude; Numerical…

The SLS Stages Intertank Structural Test Assembly (STA) arrives at MSFC.

NASA Image and Video Library

2018-03-06

The SLS Stages Intertank Structural Test Assembly (STA) is rolling off the NASA Pegasus Barge at the MSFC Dock enroute to the MSFC 4619 Load Test Annex test facility for qualification testing. STA emerges from Barge Pegasus.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ozeki, H.; Isono, T.; Uno, Y.

JAEA successfully completed the manufacture of the toroidal field (TF) insert coil (TFIC) for a performance test of the ITER TF conductor in the final design in cooperation with Hitachi, Ltd. The TFIC is a single-layer 8.875-turn solenoid coil with 1.44-m diameter. This will be tested for 68-kA current application in a 13-T external magnetic field. TFIC was manufactured in the following order: winding of the TF conductor, lead bending, fabrication of the electrical termination, heat treatment, turn insulation, installation of the coil into the support mandrel structure, vacuum pressure impregnation (VPI), structure assembly, and instrumentation. Here in this presentation,more » manufacture process and quality control status for the TFIC manufacturing are reported.« less

Test results of a 20 kA high temperature superconductor current lead using REBCO tapes

NASA Astrophysics Data System (ADS)

Heller, R.; Fietz, W. H.; Gröner, F.; Heiduk, M.; Hollik, M.; Lange, C.; Lietzow, R.

2018-05-01

The Karlsruhe Institute of Technology has developed a 20 kA high temperature superconductor (HTS) current lead (CL) using the second generation material REBCO, as industry worldwide concentrate on the production of this material. The aim was to demonstrate the possibility of replacing the Bi-2223/AgAu tapes by REBCO tapes, while for easy comparison of results, all other components are copies of the 20 kA HTS CL manufactured for the satellite tokamak JT-60SA. After the manufacture of all CL components including the newly developed REBCO module, the assembly of the CL has been executed at KIT and an experiment has been carried out in the CuLTKa test facility where the REBCO CL was installed and connected to a JT-60SA CL via a superconducting bus bar. The experiment covers steady state operation up to 20 kA, pulsed operation, measurement of the heat load at 4.5 K end, loss-of-flow-accident simulations, and quench performance studies. Here the results of these tests are reported and directly compared to those of the JT-60SA CL.

Results of EVA/mobile transporter space station truss assembly tests

NASA Technical Reports Server (NTRS)

Watson, Judith J.; Heard, Walter L., Jr.; Bush, Harold G.; Lake, M. S.; Jensen, J. K.; Wallsom, R. E.; Phelps, J. E.

1988-01-01

Underwater neutral buoyance tests were conducted to evaluate the use of a Mobile Transporter concept in conjunction with EVA astronauts to construct the Space Station Freedom truss structure. A three-bay orthogonal tetrahedral truss configuration with a 15 foot square cross section was repeatedly assembled by a single pair of pressure suited test subjects working from the Mobile Transporter astronaut positioning devices (mobile foot restraints). The average unit assembly time (which included integrated installation of utility trays) was 27.6 s/strut, or 6 min/bay. The results of these tests indicate that EVA assembly of space station size structures can be significantly enhanced when using a Mobile Transporter equipped with astronaut positioning devices. Rapid assembly time can be expected and are dependent primarily on the rate of translation permissible for on-orbit operations. The concept used to demonstate integrated installation of utility trays requires minimal EVA handling and consequentially, as the results show, has little impact on overall assembly time.

Verification Test of Automated Robotic Assembly of Space Truss Structures

NASA Technical Reports Server (NTRS)

Rhodes, Marvin D.; Will, Ralph W.; Quach, Cuong C.

1995-01-01

A multidisciplinary program has been conducted at the Langley Research Center to develop operational procedures for supervised autonomous assembly of truss structures suitable for large-aperture antennas. The hardware and operations required to assemble a 102-member tetrahedral truss and attach 12 hexagonal panels were developed and evaluated. A brute-force automation approach was used to develop baseline assembly hardware and software techniques. However, as the system matured and operations were proven, upgrades were incorporated and assessed against the baseline test results. These upgrades included the use of distributed microprocessors to control dedicated end-effector operations, machine vision guidance for strut installation, and the use of an expert system-based executive-control program. This paper summarizes the developmental phases of the program, the results of several assembly tests, and a series of proposed enhancements. No problems that would preclude automated in-space assembly or truss structures have been encountered. The test system was developed at a breadboard level and continued development at an enhanced level is warranted.

Development and verification testing of automation and robotics for assembly of space structures

NASA Technical Reports Server (NTRS)

Rhodes, Marvin D.; Will, Ralph W.; Quach, Cuong C.

1993-01-01

A program was initiated within the past several years to develop operational procedures for automated assembly of truss structures suitable for large-aperture antennas. The assembly operations require the use of a robotic manipulator and are based on the principle of supervised autonomy to minimize crew resources. A hardware testbed was established to support development and evaluation testing. A brute-force automation approach was used to develop the baseline assembly hardware and software techniques. As the system matured and an operation was proven, upgrades were incorprated and assessed against the baseline test results. This paper summarizes the developmental phases of the program, the results of several assembly tests, the current status, and a series of proposed developments for additional hardware and software control capability. No problems that would preclude automated in-space assembly of truss structures have been encountered. The current system was developed at a breadboard level and continued development at an enhanced level is warranted.

A two-pronged structural analysis of retroviral maturation indicates that core formation proceeds by a disassembly-reassembly pathway rather than a displacive transition.

PubMed

Keller, Paul W; Huang, Rick K; England, Matthew R; Waki, Kayoko; Cheng, Naiqian; Heymann, J Bernard; Craven, Rebecca C; Freed, Eric O; Steven, Alasdair C

2013-12-01

Retrovirus maturation involves sequential cleavages of the Gag polyprotein, initially arrayed in a spherical shell, leading to formation of capsids with polyhedral or conical morphology. Evidence suggests that capsids assemble de novo inside maturing virions from dissociated capsid (CA) protein, but the possibility persists of a displacive pathway in which the CA shell remains assembled but is remodeled. Inhibition of the final cleavage between CA and spacer peptide SP1/SP blocks the production of mature capsids. We investigated whether retention of SP might render CA assembly incompetent by testing the ability of Rous sarcoma virus (RSV) CA-SP to assemble in vitro into icosahedral capsids. Capsids were indeed assembled and were indistinguishable from those formed by CA alone, indicating that SP was disordered. We also used cryo-electron tomography to characterize HIV-1 particles produced in the presence of maturation inhibitor PF-46396 or with the cleavage-blocking CA5 mutation. Inhibitor-treated virions have a shell that resembles the CA layer of the immature Gag shell but is less complete. Some CA protein is generated but usually not enough for a mature core to assemble. We propose that inhibitors like PF-46396 bind to the Gag lattice where they deny the protease access to the CA-SP1 cleavage site and prevent the release of CA. CA5 particles, which exhibit no cleavage at the CA-SP1 site, have spheroidal shells with relatively thin walls. It appears that this lattice progresses displacively toward a mature-like state but produces neither conical cores nor infectious virions. These observations support the disassembly-reassembly pathway for core formation.

Self-assembly of proteins into a three-dimensional multilayer system: investigation of the surface of the human fungal pathogen Aspergillus fumigatus.

PubMed

Zykwinska, Agata; Pihet, Marc; Radji, Sadia; Bouchara, Jean-Philippe; Cuenot, Stéphane

2014-06-01

Hydrophobins are small surface active proteins that fulfil a wide spectrum of functions in fungal growth and development. The human fungal pathogen Aspergillus fumigatus expresses RodA hydrophobins that self-assemble on the outer conidial surface into tightly organized nanorods known as rodlets. AFM investigation of the conidial surface allows us to evidence that RodA hydrophobins self-assemble into rodlets through bilayers. Within bilayers, hydrophilic domains of hydrophobins point inward, thus making a hydrophilic core, while hydrophobic domains point outward. AFM measurements reveal that several rodlet bilayers are present on the conidial surface thus showing that proteins self-assemble into a complex three-dimensional multilayer system. The self-assembly of RodA hydrophobins into rodlets results from attractive interactions between stacked β-sheets, which conduct to a final linear cross-β spine structure. A Monte Carlo simulation shows that anisotropic interactions are the main driving forces leading the hydrophobins to self-assemble into parallel rodlets, which are further structured in nanodomains. Taken together, these findings allow us to propose a mechanism, which conducts RodA hydrophobins to a highly ordered rodlet structure. The mechanism of hydrophobin assembly into rodlets offers new prospects for the development of more efficient strategies leading to disruption of rodlet formation allowing a rapid detection of the fungus by the immune system. Copyright © 2014 Elsevier B.V. All rights reserved.

The advanced receiver 2: Telemetry test results in CTA 21

NASA Technical Reports Server (NTRS)

Hinedi, S.; Bevan, R.; Marina, M.

1991-01-01

Telemetry tests with the Advanced Receiver II (ARX II) in Compatibility Test Area 21 are described. The ARX II was operated in parallel with a Block-III Receiver/baseband processor assembly combination (BLK-III/BPA) and a Block III Receiver/subcarrier demodulation assembly/symbol synchronization assembly combination (BLK-III/SDA/SSA). The telemetry simulator assembly provided the test signal for all three configurations, and the symbol signal to noise ratio as well as the symbol error rates were measured and compared. Furthermore, bit error rates were also measured by the system performance test computer for all three systems. Results indicate that the ARX-II telemetry performance is comparable and sometimes superior to the BLK-III/BPA and BLK-III/SDA/SSA combinations.

Rotary mechanical latch

DOEpatents

Spletzer, Barry L.; Martinez, Michael A.; Marron, Lisa C.

2012-11-13

A rotary mechanical latch for positive latching and unlatching of a rotary device with a latchable rotating assembly having a latching gear that can be driven to latched and unlatched states by a drive mechanism such as an electric motor. A cam arm affixed to the latching gear interfaces with leading and trailing latch cams affixed to a flange within the drive mechanism. The interaction of the cam arm with leading and trailing latch cams prevents rotation of the rotating assembly by external forces such as those due to vibration or tampering.

The NIRspec assembly integration and test status

NASA Astrophysics Data System (ADS)

Wettemann, Thomas; Ehrenwinkler, Ralf; Johnson, Thomas E.; Maschmann, Marc; Mosner, Peter; te Plate, Maurice; Rödel, Andreas

2017-11-01

The Near-Infrared Spectrograph (NIRSpec) is one of the four instruments on the James Webb Space Telescope (JWST) scheduled for launch in 2018. NIRSpec has been manufactured and tested by an European industrial consortium led by Airbus Defence and Space and delivered to the European Space Agency (ESA) and NASA in September 2013. Since then it has successfully been integrated into the JWST Integrated Science Instrument Module (ISIM) and is currently in ISIM Cryo-Vacuum Test#2. Since however two of its most important assemblies, the Focal Plane Assembly (FPA) and the Micro-Shutter Assembly (MSA) need to be replaced by new units we will present the status of the instrument, the status of its new flight assemblies in manufacturing and testing and give an outlook on the planned exchange activities and the following instrument re-verification.

The SLS Stages Intertank Structural Test Assembly (STA) arrives at MSFC

NASA Image and Video Library

2018-03-08

The SLS Stages Intertank Structural Test Assembly (STA) is rolling off the NASA Pegasus Barge at the MSFC Dock enroute to the MSFC 4619 Load Test Annex test facility for qualification testing via MSFC West Test Area. STA approaches Test Stand 4693, SLS LH2 test Stand, on way to Bldg. 4619

Failure Mechanisms of Thermomechanically Loaded SnAgCu/Plastic Core Solder Ball Composite Joints in Low-Temperature Co-Fired Ceramic/Printed Wiring Board Assemblies

NASA Astrophysics Data System (ADS)

Nousiainen, O.; Putaala, J.; Kangasvieri, T.; Rautioaho, R.; Vähäkangas, J.

2007-03-01

The thermal fatigue endurance of completely lead-free 95.5Sn4Ag0.7Cu/plastic core solder ball (PCSB) composite joint structures in low-temperature Co-fired ceramic/printed wiring board (LTCC/PWB) assemblies was investigated using thermal cycling tests over the temperature ranges of -40°C 125°C and 0°C 100°C. Two separate creep/fatigue failures initiated and propagated in the joints during the tests: (1) a crack along the intermetallic compound (IMC)/solder interface on the LTCC side of the joint, which formed at the high-temperature extremes; and (2) a crack in the solder near the LTCC solder land, which formed at the low-temperature extremes. Moreover, localized recrystallization was detected at the outer edge of the joints that were tested in the harsh (-40°C 125°C) test conditions. The failure mechanism was creep/fatigue-induced mixed intergranular and transgranular cracking in the recrystallized zone, but it was dominated by transgranular thermal fatigue failure beyond the recrystallized zone. The change in the failure mechanism increased the rate of crack growth. When the lower temperature extreme was raised from -40°C to 0°C, no recrystallized zone was detected and the failure was due to intergranular cracks.

Direct Polishing of Full-Shell, High-Resolution X-Ray Optics

NASA Technical Reports Server (NTRS)

Roche, Jacqueline M.; Gubarev, Mikhail V.; Smith, W. Scott; O'Dell, Stephen L.; Kolodziejczak, Jeffrey J.; Weisskopf, Martin C.; Ramsey, Brian D.; Elsner, Ronald F.

2014-01-01

Future x-ray telescopes will likely require lightweight mirrors to attain the large collecting areas needed to accomplish the science objectives. Understanding and demonstrating processes now is critical to achieving sub-arcsecond performance in the future. Consequently, designs not only of the mirrors but of fixtures for supporting them during fabrication, metrology, handling, assembly, and testing must be adequately modeled and verified. To this end, MSFC is using finite-element modeling to study the effects of mounting on thin, full-shell grazing-incidence mirrors, during all processes leading to a flight.

The Constellation-X Focal Plane Microcalorimeter Array: An NTD-Germanium Solution

NASA Technical Reports Server (NTRS)

Beeman, J.; Silver, E.; Bandler, S.; Schnopper, H.; Murray, S.; Madden, N.; Landis, D.; Haller, E. E.; Barbera, M.

2001-01-01

The hallmarks of Neutron Transmutation Doped (NTD) germanium cryogenic thermistors include high reliability, reproducibility, and long term stability of bulk carrier transport properties. Using micro-machined NTD Ge thermistors with integral 'flying' leads, we can now fabricate two-dimensional arrays that are built up from a series of stacked linear arrays. We believe that this modular approach of building, assembling, and perhaps replacing individual modules of detectors is essential to the successful fabrication and testing of large multi-element instruments. Details of construction are presented.

Combustion Stability of the Gas Generator Assembly from J-2X Engine E10001 and Powerpack Tests

NASA Technical Reports Server (NTRS)

Hulka, J. R.; Kenny, R. L.; Casiano, M. J.

2013-01-01

Testing of a powerpack configuration (turbomachinery and gas generator assembly) and the first complete engine system of the liquid oxygen/liquid hydrogen propellant J-2X rocket engine have been completed at the NASA Stennis Space Center. The combustion stability characteristics of the gas generator assemblies on these two systems are of interest for reporting since considerable effort was expended to eliminate combustion instability during early development of the gas generator assembly with workhorse hardware. Comparing the final workhorse gas generator assembly development test data to the powerpack and engine system test data provides an opportunity to investigate how the nearly identical configurations of gas generator assemblies operate with two very different propellant supply systems one the autonomous pressure-fed test configuration on the workhorse development test stand, the other the pump-fed configurations on the powerpack and engine systems. The development of the gas generator assembly and the elimination of the combustion instability on the pressure-fed workhorse test stand have been reported extensively in the two previous Liquid Propulsion Subcommittee meetings 1-7. The powerpack and engine system testing have been conducted from mid-2011 through 2012. All tests of the powerpack and engine system gas generator systems to date have been stable. However, measureable dynamic behavior, similar to that observed on the pressure-fed test stand and reported in Ref. [6] and attributed to an injection-coupled response, has appeared in both powerpack and engine system tests. As discussed in Ref. [6], these injection-coupled responses are influenced by the interaction of the combustion chamber with a branch pipe in the hot gas duct that supplies gaseous helium to pre-spin the turbine during the start transient. This paper presents the powerpack and engine system gas generator test data, compares these data to the development test data, and provides additional combustion stability analyses of the configurations.

jsc2018m000130_Orion Crew Module for Ascent Abort-2 Arrives in Houston

NASA Image and Video Library

2018-03-08

Ascent Abort-2 Module Arrives in Houston---------------------------------------------------------- NASA’s Johnson Space Center is the center of activity leading the design and build up for a critical safety test of America’s new exploration spacecraft. An Orion crew module was delivered to Houston last week for assembly and outfitting for the April 2019 Ascent Abort-2 test, to demonstrate the ability of the spacecraft’s Launch Abort System to pull the crew module to safety if an emergency ever arises during ascent to space. Doing this work at JSC is part of a lean approach to development, to minimize cost and schedule risks associated with the test. _______________________________________ FOLLOW ORION! Twitter: https://twitter.com/NASA_Orion/ Facebook: https://www.facebook.com/NASAOrion/ Instagram: https://www.instagram.com/explorenasa/

Suppressed Sr segregation and performance of directly assembled La0.6Sr0.4Co0.2Fe0.8O3-δ oxygen electrode on Y2O3-ZrO2 electrolyte of solid oxide electrolysis cells

NASA Astrophysics Data System (ADS)

Ai, Na; He, Shuai; Li, Na; Zhang, Qi; Rickard, William D. A.; Chen, Kongfa; Zhang, Teng; Jiang, San Ping

2018-04-01

Active and stable oxygen electrode is probably the most important in the development of solid oxide electrolysis cells (SOECs) technologies. Herein, we report the successful development of mixed ionic and electronic conducting (MIEC) La0.6Sr0.4Co0.2Fe0.8O3-δ (LSCF) perovskite oxides directly assembled on barrier-layer-free yttria-stabilized zirconia (YSZ) electrolyte as highly active and stable oxygen electrodes of SOECs. Electrolysis polarization effectively induces the formation of electrode/electrolyte interface, similar to that observed under solid oxide fuel cell (SOFC) operation conditions. However, in contrast to the significant performance decay under SOFC operation conditions, the cell with directly assembled LSCF oxygen electrodes shows excellent stability, tested for 300 h at 0.5 A cm-2 and 750 °C under SOEC operation conditions. Detailed microstructure and phase analysis reveal that Sr segregation is inevitable for LSCF electrode, but anodic polarization substantially suppresses Sr segregation and migration to the electrode/electrolyte interface, leading to the formation of stable and efficient electrode/electrolyte interface for water and CO2 electrolysis under SOECs operation conditions. The present study demonstrates the feasibility of using directly assembled MIEC cobaltite based oxygen electrodes on barrier-layer-free YSZ electrolyte of SOECs.

EPRI/DOE High Burnup Fuel Sister Pin Test Plan Simplification and Visualization

DOE Office of Scientific and Technical Information (OSTI.GOV)

Saltzstein, Sylvia J.; Sorenson, Ken B.; Hanson, Brady

The EPRI/DOE High Burnup Confirmatory Data Project (herein called the "Demo") is a multi-year, multi-entity confirmation demonstration test with the purpose of providing quantitative and qualitative data to show how high-burnup fuel ages in dry storage over a ten-year period. The Demo involves obtaining 32 assemblies of high-burnup PWR fuel of four common cladding alloys from the North Anna Nuclear Power Plant, drying them according to standard plant procedures, and then storing them in an NRC-licensed TN-3 2B cask on the North Anna dry storage pad for ten years. After the ten-year storage time, the cask will be opened andmore » the rods will be examined for signs of aging. Twenty-five rods from assemblies of similar claddings, in-reactor placement, and burnup histories (herein called "sister rods") have been shipped from the North Anna Nuclear Power Plant and are currently being nondestructively tested at Oak Ridge National Laboratory. After the non-destructive testing has been completed for each of the twenty-five rods, destructive analysis will be performed at ORNL, PNNL, and ANL to obtain mechanical data. Opinions gathered from the expert interviews, ORNL and PNNL Sister Rod Test Plans, and numerous meetings has resulted in the Simplified Test Plan described in this document. Some of the opinions and discussions leading to the simplified test plan are included here. Detailed descriptions and background are in the ORNL and PNNL plans in the appendices . After the testing described in this simplified test plan h as been completed , the community will review all the collected data and determine if additional testing is needed.« less

Peroxisome Biogenesis Disorders: Biological, Clinical and Pathophysiological Perspectives

ERIC Educational Resources Information Center

Braverman, Nancy E.; D'Agostino, Maria Daniela; MacLean, Gillian E.

2013-01-01

The peroxisome biogenesis disorders (PBD) are a heterogeneous group of autosomal recessive disorders in which peroxisome assembly is impaired, leading to multiple peroxisome enzyme deficiencies, complex developmental sequelae and progressive disabilities. Mammalian peroxisome assembly involves the protein products of 16 "PEX" genes;…

Mirror image DNA nanostructures for chiral supramolecular assemblies.

PubMed

Lin, Chenxiang; Ke, Yonggang; Li, Zhe; Wang, James H; Liu, Yan; Yan, Hao

2009-01-01

L-DNA, the mirror image of natural D-DNA, can be readily self-assembled into designer discrete or periodic nanostructures. The assembly products are characterized by polyacrylamide gel electrophoresis, circular dichroism spectrum, atomic force microscope, and fluorescence microscope. We found that the use of enantiomer DNA as building material leads to the formation of DNA supramolecules with opposite chirality. Therefore, the L-DNA self-assembly is a substantial complement to the structural DNA nanotechnology. Moreover, the L-DNA architectures feature superior nuclease resistance thus are appealing for in vivo medical applications.

Fragment-Based Phenotypic Lead Discovery: Cell-Based Assay to Target Leishmaniasis.

PubMed

Ayotte, Yann; Bilodeau, François; Descoteaux, Albert; LaPlante, Steven R

2018-05-02

A rapid and practical approach for the discovery of new chemical matter for targeting pathogens and diseases is described. Fragment-based phenotypic lead discovery (FPLD) combines aspects of traditional fragment-based lead discovery (FBLD), which involves the screening of small-molecule fragment libraries to target specific proteins, with phenotypic lead discovery (PLD), which typically involves the screening of drug-like compounds in cell-based assays. To enable FPLD, a diverse library of fragments was first designed, assembled, and curated. This library of soluble, low-molecular-weight compounds was then pooled to expedite screening. Axenic cultures of Leishmania promastigotes were screened, and single hits were then tested for leishmanicidal activity against intracellular amastigote forms in infected murine bone-marrow-derived macrophages without evidence of toxicity toward mammalian cells. These studies demonstrate that FPLD can be a rapid and effective means to discover hits that can serve as leads for further medicinal chemistry purposes or as tool compounds for identifying known or novel targets. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

The Evolution of Thin-Film Structure in pi-Conjugated System: Implications for Devices

DTIC Science & Technology

2015-07-09

dependent, polymer self - assembly (Chem Matls, 2015). The results provide vital insights into factors leading to organized conjugated polymer nanostructures...34Liquid Crystalline Poly(3-hexylthiophene) Solutions Revisited: Role of Time- dependent Self - Assembly ", Chemistry of Materials (2015), 27(7), 2687-2694...period (if none, report none): For the first time, we demonstrated that π-conjugated polymers self - assemble and exhibit liquid crystal ordering

His-Tag-Mediated Dimerization of Chemoreceptors Leads to Assembly of Functional Nanoarrays.

PubMed

Haglin, Elizabeth R; Yang, Wen; Briegel, Ariane; Thompson, Lynmarie K

2017-11-07

Transmembrane chemotaxis receptors are found in bacteria in extended hexagonal arrays stabilized by the membrane and by cytosolic binding partners, the kinase CheA and coupling protein CheW. Models of array architecture and assembly propose receptors cluster into trimers of dimers that associate with one CheA dimer and two CheW monomers to form the minimal "core unit" necessary for signal transduction. Reconstructing in vitro chemoreceptor ternary complexes that are homogeneous and functional and exhibit native architecture remains a challenge. Here we report that His-tag-mediated receptor dimerization with divalent metals is sufficient to drive assembly of nativelike functional arrays of a receptor cytoplasmic fragment. Our results indicate receptor dimerization initiates assembly and precedes formation of ternary complexes with partial kinase activity. Restoration of maximal kinase activity coincides with a shift to larger complexes, suggesting that kinase activity depends on interactions beyond the core unit. We hypothesize that achieving maximal activity requires building core units into hexagons and/or coalescing hexagons into the extended lattice. Overall, the minimally perturbing His-tag-mediated dimerization leads to assembly of chemoreceptor arrays with native architecture and thus serves as a powerful tool for studying the assembly and mechanism of this complex and other multiprotein complexes.

EVA assembly of large space structure element

NASA Technical Reports Server (NTRS)

Bement, L. J.; Bush, H. G.; Heard, W. L., Jr.; Stokes, J. W., Jr.

1981-01-01

The results of a test program to assess the potential of manned extravehicular activity (EVA) assembly of erectable space trusses are described. Seventeen tests were conducted in which six "space-weight" columns were assembled into a regular tetrahedral cell by a team of two "space"-suited test subjects. This cell represents the fundamental "element" of a tetrahedral truss structure. The tests were conducted under simulated zero-gravity conditions. Both manual and simulated remote manipulator system modes were evaluated. Articulation limits of the pressure suit and zero gravity could be accommodated by work stations with foot restraints. The results of this study have confirmed that astronaut EVA assembly of large, erectable space structures is well within man's capabilities.

QUAST: quality assessment tool for genome assemblies.

PubMed

Gurevich, Alexey; Saveliev, Vladislav; Vyahhi, Nikolay; Tesler, Glenn

2013-04-15

Limitations of genome sequencing techniques have led to dozens of assembly algorithms, none of which is perfect. A number of methods for comparing assemblers have been developed, but none is yet a recognized benchmark. Further, most existing methods for comparing assemblies are only applicable to new assemblies of finished genomes; the problem of evaluating assemblies of previously unsequenced species has not been adequately considered. Here, we present QUAST-a quality assessment tool for evaluating and comparing genome assemblies. This tool improves on leading assembly comparison software with new ideas and quality metrics. QUAST can evaluate assemblies both with a reference genome, as well as without a reference. QUAST produces many reports, summary tables and plots to help scientists in their research and in their publications. In this study, we used QUAST to compare several genome assemblers on three datasets. QUAST tables and plots for all of them are available in the Supplementary Material, and interactive versions of these reports are on the QUAST website. http://bioinf.spbau.ru/quast . Supplementary data are available at Bioinformatics online.

Thermodynamic performance testing of the orbiter flash evaporator system

NASA Technical Reports Server (NTRS)

Jaax, J. R.; Melgares, M. A.; Frahm, J. P.

1980-01-01

System level testing of the space shuttle orbiter's development flash evaporator system (FES) was performed in a thermal vacuum chamber capable of simulating ambient ascent, orbital, and entry temperature and pressure profiles. The test article included the evaporator assembly, high load and topping exhaust duct and nozzle assemblies, and feedwater supply assembly. Steady state and transient heat load, water pressure/temperature and ambient pressure/temperature profiles were imposed by especially designed supporting test hardware. Testing in 1978 verified evaporator and duct heater thermal design, determined FES performance boundaries, and assessed topping evaporator plume characteristics. Testing in 1979 combined the FES with the other systems in the orbiter active thermal control subsystem (ATCS). The FES met or exceeded all nominal and contingency performance requirements during operation with the integrated ATCS. During both tests stability problems were encountered during steady state operations which resulted in subsequent design changes to the water spray nozzle and valve plate assemblies.

Katome: de novo DNA assembler implemented in rust

NASA Astrophysics Data System (ADS)

Neumann, Łukasz; Nowak, Robert M.; Kuśmirek, Wiktor

2017-08-01

Katome is a new de novo sequence assembler written in the Rust programming language, designed with respect to future parallelization of the algorithms, run time and memory usage optimization. The application uses new algorithms for the correct assembly of repetitive sequences. Performance and quality tests were performed on various data, comparing the new application to `dnaasm', `ABySS' and `Velvet' genome assemblers. Quality tests indicate that the new assembler creates more contigs than well-established solutions, but the contigs have better quality with regard to mismatches per 100kbp and indels per 100kbp. Additionally, benchmarks indicate that the Rust-based implementation outperforms `dnaasm', `ABySS' and `Velvet' assemblers, written in C++, in terms of assembly time. Lower memory usage in comparison to `dnaasm' is observed.

Confirmatory Test of the Irritant Gas Disperser, Helicopter- or Vehicle- Mounted, M5

DTIC Science & Technology

1962-07-23

disperser consists of four major assemblies: An agent-container assembly, a pressure - group assembly, a support assembly, and a throat assembly. (1) The...base. (2) The pressure - group assembly consists of two steel high-pressure tanks, secured by steel band clamps to the pressure - group support assembly...aluminum alloy tubing welded together to provide a support for the agent-container assembly and the pressure - group assembly. Each of these separate

Adapter assembly prevents damage to tubing during high pressure tests

NASA Technical Reports Server (NTRS)

Stinett, L. L.

1965-01-01

Portable adapter assembly prevents damage to tubing and injury to personnel when pressurizing a system or during high pressure tests. The assembly is capable of withstanding high pressure. It is securely attached to the tubing stub end and may be removed without brazing, cutting or cleaning the tube.

Electromagnetic compatibility test report for the tethered satellite data acquisition and control assembly

NASA Astrophysics Data System (ADS)

Hoskins, Douglas; Snead, Robert

1988-05-01

This report details the results of an electromagnetic compatibility test on the SCI Systems Data Acquisition and Control Assembly (DACA). This assembly is an electronic processor which controls the central communication link from the Tethered Satellite System (TSS) to the Space Transportation System Orbiter Space Shuttle.

Human Contamination in Public Genome Assemblies.

PubMed

Kryukov, Kirill; Imanishi, Tadashi

2016-01-01

Contamination in genome assembly can lead to wrong or confusing results when using such genome as reference in sequence comparison. Although bacterial contamination is well known, the problem of human-originated contamination received little attention. In this study we surveyed 45,735 available genome assemblies for evidence of human contamination. We used lineage specificity to distinguish between contamination and conservation. We found that 154 genome assemblies contain fragments that with high confidence originate as contamination from human DNA. Majority of contaminating human sequences were present in the reference human genome assembly for over a decade. We recommend that existing contaminated genomes should be revised to remove contaminated sequence, and that new assemblies should be thoroughly checked for presence of human DNA before submitting them to public databases.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Reaugh, J. E.

HE ignition caused by shear localization is the principal concern for safety analyses of postulated mechanical insults to explosive assemblies. Although prompt detonation from shock is certainly a concern, insults that lead to prompt detonation are associated with high velocity, and correspondingly rare. For high-density HMX assemblies, an impact speed (by a steel object) of 400 m/s is needed to develop a detonation in a run distance less than 30 mm. To achieve a steady plane shock, which results in the shortest run distance to detonation for a given peak pressure, the impactor diameter must exceed 60 mm, and thicknessmore » approach 20 mm. Thinner plates and/or smaller diameter ones require even higher impact velocity. Ignitions from shear localization, however, have been observed from impacts less than 50 m/s in Steven tests, less than 30 m/s from spigot impact tests, and less than 10 m/s from various drop tests. This lower velocity range is much frequent in postulated mechanical insults. Preliminary computer simulations and analyses of a variety of such tests have suggested that although each is accompanied by shear localization, there are differing detailed mechanisms at work that cause the ignitions. We identify those mechanisms that may be at work in a variety of such tests, and suggest how models of shear ignition, such as HERMES, may be revised and calibrated to conform to experiment. We suggest combining additional experiments with computer simulations and model development to begin confirm or uncover mechanisms that may be at work in a specific postulated event.« less

Analysis and Design of a Double-Divert Spiral Groove Seal

NASA Technical Reports Server (NTRS)

Zheng, Xiaoqing; Berard, Gerald

2007-01-01

This viewgraph presentation describes the design and analysis of a double spiral groove seal. The contents include: 1) Double Spiral Design Features; 2) Double Spiral Operational Features; 3) Mating Ring/Rotor Assembly; 4) Seal Ring Assembly; 5) Insert Segment Joints; 6) Rotor Assembly Completed Prototype Parts; 7) Seal Assembly Completed Prototype Parts; 8) Finite Element Analysis; 9) Computational Fluid Dynamics (CFD) Analysis; 10) Restrictive Orifice Design; 11) Orifice CFD Model; 12) Orifice Results; 13) Restrictive Orifice; 14) Seal Face Coning; 15) Permanent Magnet Analysis; 16) Magnetic Repulsive Force; 17) Magnetic Repulsive Test Results; 18) Spin Testing; and 19) Testing and Validation.

The AXAF technology mirror assembly program - An overview

NASA Technical Reports Server (NTRS)

Wyman, Charles L.; Dailey, Carroll C.; Reily, Cary; Weisskopf, Martin; Mckinnon, Phil

1986-01-01

The manufacture and testing of the Technology Mirror Assembly (TMA), a prototype Wolter I telescope scaled to the dimensions of the innermost element of the High-Resolution Mirror Assembly for the NASA Advanced X-ray Astrophysics Facility (AXAF), are reviewed. Consideration is given to the grinding, polishing, coating, and assembly of the zerodur TMA blanks, the TMA mount design, and the test procedures used at the MSFC X-ray Calibration Facility. Test results indicate FWHM resolution less than 0.5 arcsec, but with significant near-field scattering attributed to ripple; further long-lap polishing is suggested.

Logistics for the implementation of lead-free solders on electronic assemblies

DOE Office of Scientific and Technical Information (OSTI.GOV)

Vianco, P.T.; Artaki, I.

1993-12-31

The prospects of legislative and regulatory action aimed at taxing, restricting or banning lead-bearing materials from manufactured products has prompted the electronics community to examine the implementation of lead-free solders to replace currently used lead-containing alloys in the manufacture of electronic devices and assemblies. The logistics for changing the well established ``tin-lead solder technology`` require not only the selection of new compositions but also the qualification of different surface finishes and manufacturing processes. The meniscometer/wetting balance technique was used to evaluate the wettability of several candidate lead-free solders as well as to establish windows on processing parameters so as tomore » facilitate prototype manufacturing. Electroplated and electroless 100Sn coatings, as well as organic preservatives, were also examined as potential alternative finishes for device leads and terminations as well as circuit board conductor surfaces to replace traditional tin-lead layers. Sandia National Laboratories and AT&T have implemented a program to qualify the manufacturing feasibility of surface mount prototype circuit boards using several commercial lead-free solders by infrared reflow technology.« less

The SLS Stages Intertank Structural Test Assembly (STA) arrives at MSFC

NASA Image and Video Library

2018-03-06

The SLS Stages Intertank Structural Test Assembly (STA) is rolling off the NASA Pegasus Barge at the MSFC Dock enroute to the MSFC 4619 Load Test Annex test facility for qualification testing. STA hardware completely free of barge and flanked by tug boats.

Using phylogeny and functional traits for assessing community assembly along environmental gradients: A deterministic process driven by elevation.

PubMed

Xu, Jinshi; Chen, Yu; Zhang, Lixia; Chai, Yongfu; Wang, Mao; Guo, Yaoxin; Li, Ting; Yue, Ming

2017-07-01

Community assembly processes is the primary focus of community ecology. Using phylogenetic-based and functional trait-based methods jointly to explore these processes along environmental gradients are useful ways to explain the change of assembly mechanisms under changing world. Our study combined these methods to test assembly processes in wide range gradients of elevation and other habitat environmental factors. We collected our data at 40 plots in Taibai Mountain, China, with more than 2,300 m altitude difference in study area and then measured traits and environmental factors. Variance partitioning was used to distinguish the main environment factors leading to phylogeny and traits change among 40 plots. Principal component analysis (PCA) was applied to colligate other environment factors. Community assembly patterns along environmental gradients based on phylogenetic and functional methods were studied for exploring assembly mechanisms. Phylogenetic signal was calculated for each community along environmental gradients in order to detect the variation of trait performance on phylogeny. Elevation showed a better explanatory power than other environment factors for phylogenetic and most traits' variance. Phylogenetic and several functional structure clustered at high elevation while some conserved traits overdispersed. Convergent tendency which might be caused by filtering or competition along elevation was detected based on functional traits. Leaf dry matter content (LDMC) and leaf nitrogen content along PCA 1 axis showed conflicting patterns comparing to patterns showed on elevation. LDMC exhibited the strongest phylogenetic signal. Only the phylogenetic signal of maximum plant height showed explicable change along environmental gradients. Synthesis . Elevation is the best environment factors for predicting phylogeny and traits change. Plant's phylogenetic and some functional structures show environmental filtering in alpine region while it shows different assembly processes in middle- and low-altitude region by different trait/phylogeny. The results highlight deterministic processes dominate community assembly in large-scale environmental gradients. Performance of phylogeny and traits along gradients may be independent with each other. The novel method for calculating functional structure which we used in this study and the focus of phylogenetic signal change along gradients may provide more useful ways to detect community assembly mechanisms.

A mobile transporter concept for EVA assembly of future spacecraft

NASA Technical Reports Server (NTRS)

Watson, Judith J.; Bush, Harold G.; Heard, Walter L., Jr.; Lake, Mark S.; Jensen, J. Kermit

1990-01-01

This paper details the ground test program for the NASA Langley Research Center Mobile Transporter concept. The Mobile Transporter would assist EVA astronauts in the assembly of the Space Station Freedom. 1-g and simulated O-g (neutral buoyancy) tests were conducted to evaluate the use of the Mobile Transporter. A three-bay (44 struts) orthogonal tetrahedral truss configuration with a 15-foot-square cross section was repeatedly assembled by a single pair of pressure suited test subjects working from the Mobile Transporter astronaut positioning devices. The average unit assembly time was 28 seconds/strut. The results of these tests indicate that the use of a Mobile Transporter for EVA assembly of Space Station size structure is viable and practical. Additionally, the Mobile Transporter could be used to construct other spacecraft such as the submillimeter astronomical laboratory, space crane, and interplanetary (i.e., Mars and lunar) spacecraft.

Mars Science Laboratory Rover Integrated Pump Assembly Bellows Jamming Failure

NASA Technical Reports Server (NTRS)

Johnson, Michael R.; Johnson, Joel; Birur, Gajanana; Bhandari, Pradeep; Karlmann, Paul

2012-01-01

The Mars Science Laboratory rover and spacecraft utilize two mechanically pumped fluid loops for heat transfer to and from the internal electronics assemblies and the Radioisotope Thermo-Electric Generator (RTG). The heat transfer fluid is Freon R-11 (CFC-11) which has a large coefficient of thermal expansion. The Freon within the heat transfer system must have a volume for safe expansion of the fluid as the system temperature rises. The device used for this function is a gas-over-liquid accumulator. The accumulator uses a metal bellows to separate the fluid and gas sections. During expansion and contraction of the fluid in the system, the bellows extends and retracts to provide the needed volume change. During final testing of a spare unit, the bellows would not extend the full distance required to provide the needed expansion volume. Increasing the fluid pressure did not loosen the jammed bellows either. No amount of stroking the bellows back and forth would get it to pass the jamming point. This type of failure, if it occurred during flight, would result in significant overpressure of the heat transfer system leading to a burst failure at some point in the system piping. A loss of the Freon fluid would soon result in a loss of the mission. The determination of the source of the jamming of the bellows was quite elusive, leading to an extensive series of tests and analyses. The testing and analyses did indicate the root cause of the failure, qualitatively. The results did not provide a set of dimensional limits for the existing hardware design that would guarantee proper operation of the accumulator. In the end, a new design was developed that relied on good engineering judgment combined with the test results to select a reliable enough solution that still met other physical constraints of the hardware, the schedule, and the rover system.

Self-assembly of metal nanowires induced by alternating current electric fields

NASA Astrophysics Data System (ADS)

García-Sánchez, Pablo; Arcenegui, Juan J.; Morgan, Hywel; Ramos, Antonio

2015-01-01

We describe the reversible assembly of an aqueous suspension of metal nanowires into two different 2-dimensional stable configurations. The assembly is induced by an AC electric field of magnitude around 10 kV/m. It is known that single metal nanowires orientate parallel to the electric field for all values of applied frequency, according to two different mechanisms depending on the frequency. These different mechanisms also govern the mutual interaction between nanowires, which leads to directed-assembly into distinctive structures, the shape of which depends on the frequency of the applied field. We show that for frequencies higher than the typical frequency for charging the electrical double layer at the metal-electrolyte interface, dipole-dipole interaction leads to the formation of chains of nanowires. For lower frequencies, the nanowires form wavy bands perpendicular to the electric field direction. This behavior appears to be driven by the electroosmotic flow induced on the metal surface of the nanowires. Remarkably, no similar structures have been reported in previous studies of nanowires.

Self-Assembled Polysaccharide Nanotubes Generated from β-1,3-Glucan Polysaccharides

NASA Astrophysics Data System (ADS)

Numata, Munenori; Shinkai, Seiji

β-1,3-Glucans act as unique natural nanotubes, the features of which are greatly different from other natural or synthetic helical polymers. The origin mostly stems from their strong helix-forming nature and reversible interconversion between single-strand random coil and triple-strand helix. During this interconversion process, they can accept functional polymers, molecular assemblies and nanoparticles in an induced-fit manner to create water-soluble one-dimensional nanocomposites, where individual conjugated polymers or molecular assemblies can be incorporated into the one-dimensional hollow constructed by the helical superstructure of β-1,3-glucans. The advantageous point of the β-1,3-glucan hosting system is that the selective modification of β-1,3-glucans leads to the creation of various functional one-dimensional nanocomposites in a supramolecular manner, being applicable toward fundamental nanomaterials such as sensors or circuits. Furthermore, the composites with functional surfaces can act as one-dimensional building blocks toward further hierarchical self-assemblies, leading to the creation of two- or three-dimensional nanoarchitectures.

A Testlet Assembly Design for Adaptive Multistage Tests

ERIC Educational Resources Information Center

Luecht, Richard; Brumfield, Terry; Breithaupt, Krista

2006-01-01

This article describes multistage tests and some practical test development considerations related to the design and implementation of a multistage test, using the Uniform CPA (certified public accountant) Examination as a case study. The article further discusses the use of automated test assembly procedures in an operational context to produce…

Mechanism of asymmetric polymerase assembly at the eukaryotic replication fork

PubMed Central

Georgescu, Roxana E; Langston, Lance; Yao, Nina Y; Yurieva, Olga; Zhang, Dan; Finkelstein, Jeff; Agarwal, Tani; O’Donnell, Mike E

2015-01-01

Eukaryotes use distinct polymerases for leading- and lagging-strand replication, but how they target their respective strands is uncertain. We reconstituted Saccharomyces cerevisiae replication forks and found that CMG helicase selects polymerase (Pol) ε to the exclusion of Pol δ on the leading strand. Even if Pol δ assembles on the leading strand, Pol ε rapidly replaces it. Pol δ–PCNA is distributive with CMG, in contrast to its high stability on primed ssDNA. Hence CMG will not stabilize Pol δ, instead leaving the leading strand accessible for Pol ε and stabilizing Pol ε. Comparison of Pol ε and Pol δ on a lagging-strand model DNA reveals the opposite. Pol δ dominates over excess Pol ε on PCNA-primed ssDNA. Thus, PCNA strongly favors Pol δ over Pol ε on the lagging strand, but CMG over-rides and flips this balance in favor of Pol ε on the leading strand. PMID:24997598

Prediction of phenotypes of missense mutations in human proteins from biological assemblies.

PubMed

Wei, Qiong; Xu, Qifang; Dunbrack, Roland L

2013-02-01

Single nucleotide polymorphisms (SNPs) are the most frequent variation in the human genome. Nonsynonymous SNPs that lead to missense mutations can be neutral or deleterious, and several computational methods have been presented that predict the phenotype of human missense mutations. These methods use sequence-based and structure-based features in various combinations, relying on different statistical distributions of these features for deleterious and neutral mutations. One structure-based feature that has not been studied significantly is the accessible surface area within biologically relevant oligomeric assemblies. These assemblies are different from the crystallographic asymmetric unit for more than half of X-ray crystal structures. We find that mutations in the core of proteins or in the interfaces in biological assemblies are significantly more likely to be disease-associated than those on the surface of the biological assemblies. For structures with more than one protein in the biological assembly (whether the same sequence or different), we find the accessible surface area from biological assemblies provides a statistically significant improvement in prediction over the accessible surface area of monomers from protein crystal structures (P = 6e-5). When adding this information to sequence-based features such as the difference between wildtype and mutant position-specific profile scores, the improvement from biological assemblies is statistically significant but much smaller (P = 0.018). Combining this information with sequence-based features in a support vector machine leads to 82% accuracy on a balanced dataset of 50% disease-associated mutations from SwissVar and 50% neutral mutations from human/primate sequence differences in orthologous proteins. Copyright © 2012 Wiley Periodicals, Inc.

Isotopic Details of the Spent Catawba-1 MOX Fuel Rods at ORNL

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ellis, Ronald James

The United States Department of Energy funded Shaw/AREVA MOX Services LLC to fabricate four MOX Lead Test Assemblies (LTA) from weapons-grade plutonium. A total of four MOX LTAs (including MX03) were irradiated in the Catawba Nuclear Station (Unit 1) Catawba-1 PWR which operated at a total thermal power of 3411 MWt and had a core with 193 total fuel assemblies. The MOX LTAs were irradiated along with Duke Energy s irradiation of eight Westinghouse Next Generation Fuel (NGF) LEU LTAs (ref.1) and the remaining 181 LEU fuel assemblies. The MX03 LTA was irradiated in the Catawba-1 PWR core (refs.2,3) duringmore » cycles C-16 and C-17. C-16 began on June 5, 2005, and ended on November 11, 2006, after 499 effective full power days (EFPDs). C-17 started on December 29, 2006, (after a shutdown of 48 days) and continued for 485 EFPDs. The MX03 and three other MOX LTAs (and other fuel assemblies) were discharged at the end of C-17 on May 3, 2008. The design of the MOX LTAs was based on the (Framatome ANP, Inc.) Mark-BW/MOX1 17 17 fuel assembly design (refs. 4,5,6) for use in Westinghouse PWRs, but with MOX fuel rods with three Pu loading ranges: the nominal Pu loadings are 4.94 wt%, 3.30 wt%, and 2.40 wt%, respectively, for high, medium, and low Pu content. The Mark-BW/MOX1 (MOX LTA) fuel assembly design is the same as the Advanced Mark-BW fuel assembly design but with the LEU fuel rods replaced by MOX fuel rods (ref. 5). The fabrication of the fuel pellets and fuel rods for the MOX LTAs was performed at the Cadarache facility in France, with the fabrication of the LTAs performed at the MELOX facility, also in France.« less

Turbine seal assembly

DOEpatents

Little, David A.

2013-04-16

A seal assembly that limits gas leakage from a hot gas path to one or more disc cavities in a turbine engine. The seal assembly includes a seal apparatus that limits gas leakage from the hot gas path to a respective one of the disc cavities. The seal apparatus comprises a plurality of blade members rotatable with a blade structure. The blade members are associated with the blade structure and extend toward adjacent stationary components. Each blade member includes a leading edge and a trailing edge, the leading edge of each blade member being located circumferentially in front of the blade member's corresponding trailing edge in a direction of rotation of the turbine rotor. The blade members are arranged such that a space having a component in a circumferential direction is defined between adjacent circumferentially spaced blade members.

NASA Tests New Robotic Refueling Technologies

NASA Image and Video Library

2014-03-05

RROxiTT lead roboticist Alex Janas stands with the Oxidizer Nozzle Tool as he examines the work site. Credit: NASA/Goddard/Chris Gunn NASA has successfully concluded a remotely controlled test of new technologies that would empower future space robots to transfer hazardous oxidizer – a type of propellant – into the tanks of satellites in space today. Concurrently on the ground, NASA is incorporating results from this test and the Robotic Refueling Mission on the International Space Station to prepare for an upcoming ground-based test of a full-sized robotic servicer system that will perform tasks on a mock satellite client. Collectively, these efforts are part of an ongoing and aggressive technology development campaign to equip robots and humans with the tools and capabilities needed for spacecraft maintenance and repair, the assembly of large space telescopes, and extended human exploration. Read more here: www.nasa.gov/content/goddard/nasa-tests-new-robotic-refue... NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

Development of multichannel MEG system at IGCAR

NASA Astrophysics Data System (ADS)

Mariyappa, N.; Parasakthi, C.; Gireesan, K.; Sengottuvel, S.; Patel, Rajesh; Janawadkar, M. P.; Radhakrishnan, T. S.; Sundar, C. S.

2013-02-01

We describe some of the challenging aspects in the indigenous development of the whole head multichannel magnetoencephalography (MEG) system at IGCAR, Kalpakkam. These are: i) fabrication and testing of a helmet shaped sensor array holder of a polymeric material experimentally tested to be compatible with liquid helium temperatures, ii) the design and fabrication of the PCB adapter modules, keeping in mind the inter-track cross talk considerations between the electrical leads used to provide connections from SQUID at liquid helium temperature (4.2K) to the electronics at room temperature (300K) and iii) use of high resistance manganin wires for the 86 channels (86×8 leads) essential to reduce the total heat leak which, however, inevitably causes an attenuation of the SQUID output signal due to voltage drop in the leads. We have presently populated 22 of the 86 channels, which include 6 reference channels to reject the common mode noise. The whole head MEG system to cover all the lobes of the brain will be progressively assembled when other three PCB adapter modules, presently under fabrication, become available. The MEG system will be used for a variety of basic and clinical studies including localization of epileptic foci during pre-surgical mapping in collaboration with neurologists.

The SLS Stages Intertank Structural Test Assembly (STA) arrives at MSFC

NASA Image and Video Library

2018-03-08

The SLS Stages Intertank Structural Test Assembly (STA) is rolling off the NASA Pegasus Barge at the MSFC Dock enroute to the MSFC 4619 Load Test Annex test facility for qualification testing via MSFC West Test Area. Historic Saturn 1-C test stand on far left, blockhouse 4670 on far right, SLS LH2 test stand, 4693, in center.

The SLS Stages Intertank Structural Test Assembly (STA) arrives at MSFC

NASA Image and Video Library

2018-03-08

The SLS Stages Intertank Structural Test Assembly (STA) is rolling off the NASA Pegasus Barge at the MSFC Dock enroute to the MSFC 4619 Load Test Annex test facility for qualification testing via MSFC West Test Area. STA enters West Test Area from intersection of Dodd and Saturn roads. Onlookers take photos with Historic Dynamic Test Stand in background.

Multiparameter vision testing apparatus

NASA Technical Reports Server (NTRS)

Hunt, S. R., Jr.; Homkes, R. J.; Poteate, W. B.; Sturgis, A. C. (Inventor)

1975-01-01

Compact vision testing apparatus is described for testing a large number of physiological characteristics of the eyes and visual system of a human subject. The head of the subject is inserted into a viewing port at one end of a light-tight housing containing various optical assemblies. Visual acuity and other refractive characteristics and ocular muscle balance characteristics of the eyes of the subject are tested by means of a retractable phoroptor assembly carried near the viewing port and a film cassette unit carried in the rearward portion of the housing (the latter selectively providing a variety of different visual targets which are viewed through the optical system of the phoroptor assembly). The visual dark adaptation characteristics and absolute brightness threshold of the subject are tested by means of a projector assembly which selectively projects one or both of a variable intensity fixation target and a variable intensity adaptation test field onto a viewing screen located near the top of the housing.

Flip-chip assembly and reliability using gold/tin solder bumps

NASA Astrophysics Data System (ADS)

Oppermann, Hermann; Hutter, Matthias; Klein, Matthias; Reichl, Herbert

2004-09-01

Au/Sn solder bumps are commonly used for flip chip assembly of optoelectronic and RF devices. They allow a fluxless assembly which is required to avoid contamination at optical interfaces. Flip chip assembly experiments were carried out using as plated Au/Sn bumps without prior bump reflow. An RF and reliability test vehicles comprise a GaAs chip which was flip chip soldered on a silicon substrate. Temperature cycling tests with and without underfiller were performed and the results are presented. The different failure modes for underfilled and non-underfilled samples were discussed and compared. Additional reliability tests were performed with flip chip bonding by gold thermocompression for comparison. The test results and the failure modes are discussed in detail.

Many-molecule encapsulation by an icosahedral shell

PubMed Central

Perlmutter, Jason D; Mohajerani, Farzaneh; Hagan, Michael F

2016-01-01

We computationally study how an icosahedral shell assembles around hundreds of molecules. Such a process occurs during the formation of the carboxysome, a bacterial microcompartment that assembles around many copies of the enzymes ribulose 1,5-bisphosphate carboxylase/ oxygenase and carbonic anhydrase to facilitate carbon fixation in cyanobacteria. Our simulations identify two classes of assembly pathways leading to encapsulation of many-molecule cargoes. In one, shell assembly proceeds concomitantly with cargo condensation. In the other, the cargo first forms a dense globule; then, shell proteins assemble around and bud from the condensed cargo complex. Although the model is simplified, the simulations predict intermediates and closure mechanisms not accessible in experiments, and show how assembly can be tuned between these two pathways by modulating protein interactions. In addition to elucidating assembly pathways and critical control parameters for microcompartment assembly, our results may guide the reengineering of viruses as nanoreactors that self-assemble around their reactants. DOI: http://dx.doi.org/10.7554/eLife.14078.001 PMID:27166515

Self-assembly assisted polymerization (SAAP): approaching long multi-block copolymers with an ordered chain sequence and controllable block length.

PubMed

Wu, Chi; Xie, Zuowei; Zhang, Guangzhao; Zi, Guofu; Tu, Yingfeng; Yang, Yali; Cai, Ping; Nie, Ting

2002-12-07

A combination of polymer physics and synthetic chemistry has enabled us to develop self-assembly assisted polymerization (SAAP), leading to the preparation of long multi-block copolymers with an ordered chain sequence and controllable block lengths.

Entropy driven key-lock assembly

NASA Astrophysics Data System (ADS)

Odriozola, G.; Jiménez-Ángeles, F.; Lozada-Cassou, M.

2008-09-01

The effective interaction between a sphere with an open cavity (lock) and a spherical macroparticle (key), both immersed in a hard sphere fluid, is studied by means of Monte Carlo simulations. As a result, a two-dimensional map of the key-lock effective interaction potential is constructed, which leads to the proposal of a self-assembling mechanism: There exists trajectories through which the key-lock pair could assemble avoiding trespassing potential barriers. Hence, solely the entropic contribution can induce their self-assembling even in the absence of attractive forces. This study points out the solvent contribution within the underlying mechanisms of substrate-protein assembly/disassembly processes, which are important steps of the enzyme catalysis and protein mediated transport.

Assembly/Disassembly of DNA-Au Nanoparticles: A Strategy of Intervention

DOE PAGES

Lim, I-Im S.; Wang, Lingyan; Chandrachud, Uma; ...

2008-01-01

This report describes the viability of a strategy for manipulating the assembly/disassembly processes of DNA-Au nanoparticles by molecular intervention. Using the temperature-induced assembly and disassembly processes of DNAs and gold nanoparticles as a model system, the introduction of a molecular recognition probe is demonstrated to lead to the intervention of the assembly/disassembly processes depending on its specific biorecognition. This process can be detected by monitoring the change in the optical properties of gold nanoparticles and their DNA assemblies. Implications of the preliminary results to exploration of the resulting nanostructures for fine-tuning of the interfacial reactivities in DNA-based bioassays and biomaterialmore » engineering are also discussed.« less

49 CFR 572.192 - Head assembly.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Head assembly. 572.192 Section 572.192... Test Dummy, Small Adult Female § 572.192 Head assembly. (a) The head assembly consists of the head (180...) of this section, the head assembly shall meet performance requirements specified in paragraph (c) of...

49 CFR 572.182 - Head assembly.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Head assembly. 572.182 Section 572.182... Test Dummy, 50th Percentile Adult Male § 572.182 Head assembly. (a) The head assembly consists of the..., the head assembly shall meet performance requirements specified in paragraph (c) of this section. (b...

49 CFR 572.182 - Head assembly.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 7 2010-10-01 2010-10-01 false Head assembly. 572.182 Section 572.182... Dummy, 50th Percentile Adult Male § 572.182 Head assembly. (a) The head assembly consists of the head... assembly shall meet performance requirements specified in paragraph (c) of this section. (b) Test procedure...

49 CFR 572.182 - Head assembly.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Head assembly. 572.182 Section 572.182... Dummy, 50th Percentile Adult Male § 572.182 Head assembly. (a) The head assembly consists of the head... assembly shall meet performance requirements specified in paragraph (c) of this section. (b) Test procedure...

49 CFR 572.192 - Head assembly.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Head assembly. 572.192 Section 572.192... Test Dummy, Small Adult Female § 572.192 Head assembly. (a) The head assembly consists of the head (180...) of this section, the head assembly shall meet performance requirements specified in paragraph (c) of...

49 CFR 572.182 - Head assembly.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Head assembly. 572.182 Section 572.182... Dummy, 50th Percentile Adult Male § 572.182 Head assembly. (a) The head assembly consists of the head... assembly shall meet performance requirements specified in paragraph (c) of this section. (b) Test procedure...

49 CFR 572.182 - Head assembly.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Head assembly. 572.182 Section 572.182... Test Dummy, 50th Percentile Adult Male § 572.182 Head assembly. (a) The head assembly consists of the..., the head assembly shall meet performance requirements specified in paragraph (c) of this section. (b...

Integrating Test-Form Formatting into Automated Test Assembly

ERIC Educational Resources Information Center

Diao, Qi; van der Linden, Wim J.

2013-01-01

Automated test assembly uses the methodology of mixed integer programming to select an optimal set of items from an item bank. Automated test-form generation uses the same methodology to optimally order the items and format the test form. From an optimization point of view, production of fully formatted test forms directly from the item pool using…

49 CFR 572.166 - Knees and knee impact test procedure.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 7 2010-10-01 2010-10-01 false Knees and knee impact test procedure. 572.166... Hybrid III Six-Year-Old Weighted Child Test Dummy § 572.166 Knees and knee impact test procedure. The knee assembly is assembled and tested as specified in 49 CFR 572.126 (Subpart N). ...

Evaluating Statistical Targets for Assembling Parallel Mixed-Format Test Forms

ERIC Educational Resources Information Center

Debeer, Dries; Ali, Usama S.; van Rijn, Peter W.

2017-01-01

Test assembly is the process of selecting items from an item pool to form one or more new test forms. Often new test forms are constructed to be parallel with an existing (or an ideal) test. Within the context of item response theory, the test information function (TIF) or the test characteristic curve (TCC) are commonly used as statistical…

7. PHOTOCOPY, PLANS, ELEVATIONS, AND SECTION DRAWING FOR MISSILE TEST ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. PHOTOCOPY, PLANS, ELEVATIONS, AND SECTION DRAWING FOR MISSILE TEST AND ASSEMBLY BUILDING. - NIKE Missile Base SL-40, Missile Test & Assembly Building, South end of launch area, northeast of Generator Building No. 3, Hecker, Monroe County, IL

International Space Station Carbon Dioxide Removal Assembly Testing

NASA Technical Reports Server (NTRS)

Knox, James C.

2000-01-01

Performance testing of the International Space Station Carbon Dioxide Removal Assembly flight hardware in the United States Laboratory during 1999 is described. The CDRA exceeded carbon dioxide performance specifications and operated flawlessly. Data from this test is presented.

49 CFR 572.175 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2012 CFR

2012-10-01

.../lower torso assembly is as follows: (1) Torque the lumbar cable (drawing 420-4130) (incorporated by... threaded attachment holes. Tighten the mountings so that the test material is rigidly affixed to the test...

49 CFR 572.175 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2013 CFR

2013-10-01

.../lower torso assembly is as follows: (1) Torque the lumbar cable (drawing 420-4130) (incorporated by... threaded attachment holes. Tighten the mountings so that the test material is rigidly affixed to the test...

49 CFR 572.175 - Upper and lower torso assemblies and torso flexion test procedure.

Code of Federal Regulations, 2014 CFR

2014-10-01

.../lower torso assembly is as follows: (1) Torque the lumbar cable (drawing 420-4130) (incorporated by... threaded attachment holes. Tighten the mountings so that the test material is rigidly affixed to the test...

Development of assembly techniques for fire resistant aircraft interior panels

NASA Technical Reports Server (NTRS)

Lee, S. C. S.

1978-01-01

Ten NASA Type A fire resistant aircraft interior panels were fabricated and tested to develop assembly techniques. These techiques were used in the construction of a full scale lavatory test structure for flame propagation testing. The Type A panel is of sandwich construction consisting of Nomex honeycomb filled with quinone dioxime foam, and bismaleimide/glass face sheets bonded to the core with polyimide film adhesive. The materials selected and the assembly techniques developed for the lavatory test structure were designed for obtaining maximum fire containment with minimum smoke and toxic emission.

Internally damped, self-arresting vertical drop-weight apparatus

NASA Technical Reports Server (NTRS)

Ambur, Damodar R. (Inventor); Prasad, Chunchu B. (Inventor); Waters, William A. (Inventor); Stockum, Robert W. (Inventor); Walter, Manfred A. (Inventor)

1994-01-01

A vertical dropped-weight impact test machine has a dropped-weight barrel vertically supported on upper and lower support brackets. The dropped-weight barrel is chambered to receive a dropped-weight assembly having a latch pin at its upper end, a damping unit in the middle, and a tup at its lower end. The tup is adapted for gathering data during impact testing. The latch pin releasably engages a latch pin coupling assembly. The latch pin coupling assembly is attached to a winch via a halyard for raising and lowering the dropped-weight assembly. The lower end of the dropped-weight barrel is provided with a bounce-back arresting mechanism which is activated by the descending passage of the dropped-weight assembly. After striking the specimen, the dropped-weight assembly rebounds vertically and is caught by the bounce-back arresting mechanism. The damping unit of the dropped-weight assembly serves to dissipate energy from the rebounding dropped-weight assembly and prevents the dropped-weight assembly from rebounding from the self-arresting mechanism.

Fabrication of fuel pin assemblies, phase 3

NASA Technical Reports Server (NTRS)

Keeton, A. R.; Stemann, L. G.

1972-01-01

Five full size and eight reduced length fuel pins were fabricated for irradiation testing to evaluate design concepts for a fast spectrum lithium cooled compact space power reactor. These assemblies consisted of uranium mononitride fuel pellets encased in a T-111 (Ta-8W-2Hf) clad with a tungsten barrier separating fuel and clad. Fabrication procedures were fully qualified by process development and assembly qualification tests. Detailed specifications and procedures were written for the fabrication and assembly of prototype fuel pins.

James Webb Space Telescope: Frequently Asked Questions for Scientists and Engineers

NASA Technical Reports Server (NTRS)

Gardner, Jonathan P.

2008-01-01

JWST will be tested incrementally during its construction, starting with individual mirrors and instruments (including cameras and spectrometers) and building up to the full observatory. JWST's mirrors and the telescope structure are first each tested individually, including optical testing of the mirrors and alignment testing of the structure inside a cold thermal-vacuum chamber. The mirrors are then installed on the telescope structure in a clean room at Goddard Space Flight Center (GSFC). In parallel to the telescope assembly and alignment, the instruments are being built and tested, again first individually, and then as part of an integrated instrument assembly. The integrated instrument assembly will be tested in a thermal-vacuum chamber at GSFC using an optical simulator of the telescope. This testing makes sure the instruments are properly aligned relative to each other and also provides an independent check of the individual tests. After both the telescope and the integrated instrument module are successfully assembled, the integrated instrument module will be installed onto the telescope, and the combined system will be sent to Johnson Space Flight Center (JSC) where it will be optically tested in one of the JSC chambers. The process includes testing the 18 primary mirror segments acting as a single primary mirror, and testing the end-to-end system. The final system test will assure that the combined telescope and instruments are focused and aligned properly, and that the alignment, once in space, will be within the range of the actively controlled optics. In general, the individual optical tests of instruments and mirrors are the most accurate. The final system tests provide a cost-effective check that no major problem has occurred during assembly. In addition, independent optical checks of earlier tests will be made as the full system is assembled, providing confidence that there are no major problems.

NEUTRONIC REACTOR BURIAL ASSEMBLY

DOEpatents

Treshow, M.

1961-05-01

A burial assembly is shown whereby an entire reactor core may be encased with lead shielding, withdrawn from the reactor site and buried. This is made possible by a five-piece interlocking arrangement that may be easily put together by remote control with no aligning of bolt holes or other such close adjustments being necessary.

A simulation facility for testing Space Station assembly procedures

NASA Technical Reports Server (NTRS)

Hajare, Ankur R.; Wick, Daniel T.; Shehad, Nagy M.

1994-01-01

NASA plans to construct the Space Station Freedom (SSF) in one of the most hazardous environments known to mankind - space. It is of the utmost importance that the procedures to assemble and operate the SSF in orbit are both safe and effective. This paper describes a facility designed to test the integration of the telerobotic systems and to test assembly procedures using a real-world robotic arm grappling space hardware in a simulated microgravity environment.

Development of environmentally conscious cleaning process for leadless chip carrier assemblies. Final report

DOE Office of Scientific and Technical Information (OSTI.GOV)

Adams, B.E.

1995-04-01

A cross-functional team of process, product, quality, material, and design lab engineers was assembled to develop an environmentally friendly cleaning process for leadless chip carrier assemblies (LCCAs). Using flush and filter testing, Auger surface analysis, GC-Mass spectrophotometry, production yield results, and electrical testing results over an extended testing period, the team developed an aqueous cleaning process for LCCAs. The aqueous process replaced the Freon vapor degreasing/ultrasonic rinse process.

Radiated Emissions from a Remote-Controlled Airplane-Measured in a Reverberation Chamber

NASA Technical Reports Server (NTRS)

Ely, Jay J.; Koppen, Sandra V.; Nguyen, Truong X.; Dudley, Kenneth L.; Szatkowski, George N.; Quach, Cuong C.; Vazquez, Sixto L.; Mielnik, John J.; Hogge, Edward F.; Hill, Boyd L.;

Acoustic measurements on aerofoils moving in a circle at high speed

NASA Technical Reports Server (NTRS)

Wright, S. E.; Crosby, W.; Lee, D. L.

1982-01-01

Features of the test apparatus, research objectives and sample test results at the Stanford University rotor aerodynamics and noise facility are described. A steel frame equipped to receive lead shot for damping vibrations supports the drive shaft for rotor blade elements. Sleeve bearings are employed to assure quietness, and a variable speed ac motor produces the rotations. The test stand can be configured for horizontal or vertical orientation of the drive shaft. The entire assembly is housed in an acoustically sealed room. Rotation conditions for hover and large angles of attack can be studied, together with rotational and blade element noises. Research is possible on broad band, discrete frequency, and high speed noise, with measurements taken 3 m from the center of the rotor. Acoustic signatures from Mach 0.3-0.93 trials with a NACA 0012 airfoil are provided.

Subscale Testing of a Ceramic Composite Cooled Panel Led to Its Design and Fabrication for Scramjet Engine Testing

NASA Technical Reports Server (NTRS)

Jaskowiak, Martha H.

2004-01-01

In a partnership between the NASA Glenn Research Center and Pratt & Whitney, a ceramic heat exchanger panel intended for use along the hot-flow-path walls of future reusable launch vehicles was designed, fabricated, and tested. These regeneratively cooled ceramic matrix composite (CMC) panels offer lighter weight, higher operating temperatures, and reduced coolant requirements in comparison to their more traditional metallic counterparts. A maintainable approach to the design was adopted which allowed the panel components to be assembled with high-temperature fasteners rather than by permanent bonding methods. With this approach, the CMC hot face sheet, the coolant containment system, and backside structure were all fabricated separately and could be replaced individually as the need occurred during use. This maintainable design leads to both ease of fabrication and reduced cost.

Introduction and Validation of Chromium-Free Consumables for Welding Stainless Steels. Version 2

DTIC Science & Technology

2015-04-14

12 4.3 Site-related Permits and Regulations .......................................................................... 13 TEST DESIGN ...109 Appendix K: X-ray Report on ENiCuRu Weld Test Assembly...60 Table 6.24 Radiographic Test Report on Baseline E308L-16 and Test ENiCuRu Weld Assemblies

Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles.

PubMed

Acuña, Rodrigo; Cifuentes-Muñoz, Nicolás; Márquez, Chantal L; Bulling, Manuela; Klingström, Jonas; Mancini, Roberta; Lozach, Pierre-Yves; Tischler, Nicole D

2014-02-01

How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.

Modular robotic assembly of small devices.

PubMed

Frauenfelder, M

2000-01-01

The use of robots for the automatic assembly of devices of up to 100 x 100 x 100 mm is relatively uncommon today. Insufficient return on investment and the long lead times that are required have been limiting factors. Innovations in vision technology have led to the development of robotic assembly systems that employ flexible part-feeding. The benefits of these systems are described, which suggest that better ratios of price to productivity and deployment times are now achievable.

Morphogenetic Pathway of Spore Wall Assembly in Saccharomyces cerevisiae

PubMed Central

Coluccio, Alison; Bogengruber, Edith; Conrad, Michael N.; Dresser, Michael E.; Briza, Peter; Neiman, Aaron M.

2004-01-01

The Saccharomyces cerevisiae spore is protected from environmental damage by a multilaminar extracellular matrix, the spore wall, which is assembled de novo during spore formation. A set of mutants defective in spore wall assembly were identified in a screen for mutations causing sensitivity of spores to ether vapor. The spore wall defects in 10 of these mutants have been characterized in a variety of cytological and biochemical assays. Many of the individual mutants are defective in the assembly of specific layers within the spore wall, leading to arrests at discrete stages of assembly. The localization of several of these gene products has been determined and distinguishes between proteins that likely are involved directly in spore wall assembly and probable regulatory proteins. The results demonstrate that spore wall construction involves a series of dependent steps and provide the outline of a morphogenetic pathway for assembly of a complex extracellular structure. PMID:15590821

Evaluation of Hardware and Procedures for Astronaut Assembly and Repair of Large Precision Reflectors

NASA Technical Reports Server (NTRS)

Lake, Mark S.; Heard, Walter L., Jr.; Watson, Judith J.; Collins, Timothy J.

2000-01-01

A detailed procedure is presented that enables astronauts in extravehicular activity (EVA) to efficiently assemble and repair large (i.e., greater than 10m-diameter) segmented reflectors, supported by a truss, for space-based optical or radio-frequency science instruments. The procedure, estimated timelines, and reflector hardware performance are verified in simulated 0-g (neutral buoyancy) assembly tests of a 14m-diameter, offset-focus, reflector test article. The test article includes a near-flight-quality, 315-member, doubly curved support truss and 7 mockup reflector panels (roughly 2m in diameter) representing a portion of the 37 total panels needed to fully populate the reflector. Data from the tests indicate that a flight version of the design (including all reflector panels) could be assembled in less than 5 hours - less than the 6 hours normally permitted for a single EVA. This assembly rate essentially matches pre-test predictions that were based on a vast amount of historical data on EVA assembly of structures produced by NASA Langley Research Center. Furthermore, procedures and a tool for the removal and replacement of a damaged reflector panel were evaluated, and it was shown that EVA repair of this type of reflector is feasible with the use of appropriate EVA crew aids.

Safety Evaluation of Self-assembling Peptide Gel after Intracranial Administration to Rats Using the Open Field Test.

PubMed

Tsunoda, Masashi; Sugaya, Chiemi; Sugiura, Yumiko; Nagai, Yusuke; Sakanishi, Kotaro

2016-01-01

Self-assembling peptides have been developed as clinical materials, which could scaffold to regenerate nerve cells and hemostatic materials in vivo. However, there has not been enough information for their in vivo application. The safety of self-assembling peptides for the application on the brain was examined using behavioral tests for each rat in this study. Self-assembling peptide gel was administered to the surface of the brain at a volume of 20 µL at 1.5%. After 2 months, the open field test and the prepulse inhibition (PPI) test were performed. There were no significant differences between the peptide gel and the control groups in locomotor distances and in %PPIs in the PPI test. The mean values of the percentage of time the rats stayed in the central area of the open field during the first 5 min and instances of center rearing or face washing in the peptide gel group were significantly higher than those in the control. There were amorphous substance in the subarachnoid region, and infiltrations of mononuclear cells were also observed in the self-assembling peptide gel group. Although the meaning of the effects observed in this study was not fully elucidated, the self-assembling gel produced marginal but significant behavioral and histological effects.

ZPR-6 assembly 7 high {sup 240} PU core : a cylindrical assemby with mixed (PU, U)-oxide fuel and a central high {sup 240} PU zone.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lell, R. M.; Schaefer, R. W.; McKnight, R. D.

Over a period of 30 years more than a hundred Zero Power Reactor (ZPR) critical assemblies were constructed at Argonne National Laboratory. The ZPR facilities, ZPR-3, ZPR-6, ZPR-9 and ZPPR, were all fast critical assembly facilities. The ZPR critical assemblies were constructed to support fast reactor development, but data from some of these assemblies are also well suited to form the basis for criticality safety benchmarks. Of the three classes of ZPR assemblies, engineering mockups, engineering benchmarks and physics benchmarks, the last group tends to be most useful for criticality safety. Because physics benchmarks were designed to test fast reactormore » physics data and methods, they were as simple as possible in geometry and composition. The principal fissile species was {sup 235}U or {sup 239}Pu. Fuel enrichments ranged from 9% to 95%. Often there were only one or two main core diluent materials, such as aluminum, graphite, iron, sodium or stainless steel. The cores were reflected (and insulated from room return effects) by one or two layers of materials such as depleted uranium, lead or stainless steel. Despite their more complex nature, a small number of assemblies from the other two classes would make useful criticality safety benchmarks because they have features related to criticality safety issues, such as reflection by soil-like material. The term 'benchmark' in a ZPR program connotes a particularly simple loading aimed at gaining basic reactor physics insight, as opposed to studying a reactor design. In fact, the ZPR-6/7 Benchmark Assembly (Reference 1) had a very simple core unit cell assembled from plates of depleted uranium, sodium, iron oxide, U3O8, and plutonium. The ZPR-6/7 core cell-average composition is typical of the interior region of liquid-metal fast breeder reactors (LMFBRs) of the era. It was one part of the Demonstration Reactor Benchmark Program,a which provided integral experiments characterizing the important features of demonstration-size LMFBRs. As a benchmark, ZPR-6/7 was devoid of many 'real' reactor features, such as simulated control rods and multiple enrichment zones, in its reference form. Those kinds of features were investigated experimentally in variants of the reference ZPR-6/7 or in other critical assemblies in the Demonstration Reactor Benchmark Program.« less

QUAST: quality assessment tool for genome assemblies

PubMed Central

Gurevich, Alexey; Saveliev, Vladislav; Vyahhi, Nikolay; Tesler, Glenn

2013-01-01

Summary: Limitations of genome sequencing techniques have led to dozens of assembly algorithms, none of which is perfect. A number of methods for comparing assemblers have been developed, but none is yet a recognized benchmark. Further, most existing methods for comparing assemblies are only applicable to new assemblies of finished genomes; the problem of evaluating assemblies of previously unsequenced species has not been adequately considered. Here, we present QUAST—a quality assessment tool for evaluating and comparing genome assemblies. This tool improves on leading assembly comparison software with new ideas and quality metrics. QUAST can evaluate assemblies both with a reference genome, as well as without a reference. QUAST produces many reports, summary tables and plots to help scientists in their research and in their publications. In this study, we used QUAST to compare several genome assemblers on three datasets. QUAST tables and plots for all of them are available in the Supplementary Material, and interactive versions of these reports are on the QUAST website. Availability: http://bioinf.spbau.ru/quast Contact: gurevich@bioinf.spbau.ru Supplementary information: Supplementary data are available at Bioinformatics online. PMID:23422339

One-dimensional poly(L-lysine)-block-poly(L-threonine) assemblies exhibit potent anticancer activity by enhancing membranolysis.

PubMed

Chen, Yu-Fon; Shiau, Ai-Li; Chang, Sue-Joan; Fan, Nai-Shin; Wang, Chung-Teng; Wu, Chao-Liang; Jan, Jeng-Shiung

2017-06-01

Herein, we report the oncolytic activity of cationic, one-dimensional (1D) fibril assemblies formed from coil-sheet poly(L-lysine)-block-poly(L-threonine) (PLL-b-PLT) block copolypeptides for cancer therapy. The 1D fibril assemblies can efficiently interact with negatively charged cellular and mitochondrial membranes via electrostatic interactions, leading to necrosis via membrane lysis and apoptosis via the mitochondria-lytic effect. The concept is analogous to that of 1D drug carriers that exhibit enhanced cell penetration. In comparison to free PLL chains, PLL-b-PLT fibril assemblies exhibit selective cytotoxicity toward cancer cells, low hemolysis activity, enhanced membranolytic activity, and a different apoptosis pathway, which may be due to differences in the peptide-membrane interactions. Antitumor studies using a metastatic LL2 lung carcinoma model indicate that the fibril assemblies significantly inhibited tumor growth, improved survival in tumor-bearing mice and suppressed lung metastasis without obvious body weight loss. An additive efficacy was also observed for treatment with both PLL-b-PLT and cisplatin. These results support the feasibility of using 1D fibril assemblies as potential apoptotic anticancer therapeutics. We report that cationic, one-dimensional (1D) fibril assemblies formed by coil-sheet poly(L-lysine)-block-poly(L-threonine) (PLL-b-PLT) block copolypeptides exhibited potent anticancer activity by enhancing membranolysis. The 1D fibril assemblies can efficiently interact with negatively charged cellular and mitochondrial membranes via electrostatic interactions, leading to necrosis via membrane lysis and apoptosis via mitochondria-lytic effect. Moreover, the fibril assemblies exhibited low hemolytic activity and selective cytotoxicity toward cancer cell, which is advantageous as compared to PLL and most antimicrobial/anticancerous peptides. This study provides a new concept of using cationic, 1D fibril assemblies for cancer therapy. Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

49 CFR 572.186 - Abdomen assembly.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Abdomen assembly. 572.186 Section 572.186... Test Dummy, 50th Percentile Adult Male § 572.186 Abdomen assembly. (a) The abdomen assembly (175-5000) is part of the dummy assembly shown in drawing 175-0000 including load sensors specified in § 572.189...

49 CFR 572.112 - Head assembly.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Head assembly. 572.112 Section 572.112... 50th Percentile Male § 572.112 Head assembly. The head assembly consists of the head (drawing 78051-61X...) accelerometers that are mounted in conformance to § 572.36 (c). (a) Test procedure. (1) Soak the head assembly in...

49 CFR 572.112 - Head assembly.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Head assembly. 572.112 Section 572.112... 50th Percentile Male § 572.112 Head assembly. The head assembly consists of the head (drawing 78051-61X...) accelerometers that are mounted in conformance to § 572.36 (c). (a) Test procedure. (1) Soak the head assembly in...

49 CFR 572.112 - Head assembly.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 7 2010-10-01 2010-10-01 false Head assembly. 572.112 Section 572.112... 50th Percentile Male § 572.112 Head assembly. The head assembly consists of the head (drawing 78051-61X...) accelerometers that are mounted in conformance to § 572.36 (c). (a) Test procedure. (1) Soak the head assembly in...

49 CFR 572.112 - Head assembly.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Head assembly. 572.112 Section 572.112... 50th Percentile Male § 572.112 Head assembly. The head assembly consists of the head (drawing 78051-61X...) accelerometers that are mounted in conformance to § 572.36 (c). (a) Test procedure. (1) Soak the head assembly in...

49 CFR 572.112 - Head assembly.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Head assembly. 572.112 Section 572.112... 50th Percentile Male § 572.112 Head assembly. The head assembly consists of the head (drawing 78051-61X...) accelerometers that are mounted in conformance to § 572.36 (c). (a) Test procedure. (1) Soak the head assembly in...

Detail design of the surface tension propellant management device for the Intelsat VII communication satellite

NASA Astrophysics Data System (ADS)

Giacalone, Philip L.

1993-06-01

The design of the Intelsat VII surface tension propellant management device (PMD) (an all-welded assembly consisting of about 100 individual components) was developed using a modular design approach that allowed the complex PMD assembly to be divided into smaller modules. The modular approach reduces manufacturing-related technical and schedule risks and allows many components and assemblies to be processed in parallel, while also facilitating the incorporation of quality assurance tests at all critical PMD subassembly levels. The baseline PMD assembly is made from titanium and stainless steel materials. In order to obtain a 100 percent titanium PMD, a new, state-of-the-art fine mesh titanium screen material was developed, tested, and qualified for use as an alternaltive to the stainless steel screen material. The Ti based screen material demonstrated a high level of bubble point performance. It was integrated into a PMD assembly and was successfully qualification tested at the tank assembly level.

Baseline tests of an autonomous telerobotic system for assembly of space truss structures

NASA Technical Reports Server (NTRS)

Rhodes, Marvin D.; Will, Ralph W.; Quach, Coung

1994-01-01

Several proposed space missions include precision reflectors that are larger in diameter than any current or proposed launch vehicle. Most of these reflectors will require a truss structure to accurately position the reflector panels and these reflectors will likely require assembly in orbit. A research program has been conducted at the NASA Langley Research Center to develop the technology required for the robotic assembly of truss structures. The focus of this research has been on hardware concepts, computer software control systems, and operator interfaces necessary to perform supervised autonomous assembly. A special facility was developed and four assembly and disassembly tests of a 102-strut tetrahedral truss have been conducted. The test procedures were developed around traditional 'pick-and-place' robotic techniques that rely on positioning repeatability for successful operation. The data from two of the four tests were evaluated and are presented in this report. All operations in the tests were controlled by predefined sequences stored in a command file, and the operator intervened only when the system paused because of the failure of an actuator command. The tests were successful in identifying potential pitfalls in a telerobotic system, many of which would not have been readily anticipated or incurred through simulation studies. Addressing the total integrated task, instead of bench testing the component parts, forced all aspects of the task to be evaluated. Although the test results indicate that additional developments should be pursued, no problems were encountered that would preclude automated assembly in space as a viable construction method.

Unique Proton Transportation Pathway in a Robust Inorganic Coordination Polymer Leading to Intrinsically High and Sustainable Anhydrous Proton Conductivity.

PubMed

Gui, Daxiang; Dai, Xing; Tao, Zetian; Zheng, Tao; Wang, Xiangxiang; Silver, Mark A; Shu, Jie; Chen, Lanhua; Wang, Yanlong; Zhang, Tiantian; Xie, Jian; Zou, Lin; Xia, Yuanhua; Zhang, Jujia; Zhang, Jin; Zhao, Ling; Diwu, Juan; Zhou, Ruhong; Chai, Zhifang; Wang, Shuao

2018-05-16

Although comprehensive progress has been made in the area of coordination polymer (CP)/metal-organic framework (MOF)-based proton-conducting materials over the past decade, searching for a CP/MOF with stable, intrinsic, high anhydrous proton conductivity that can be directly used as a practical electrolyte in an intermediate-temperature proton-exchange membrane fuel cell assembly for durable power generation remains a substantial challenge. Here, we introduce a new proton-conducting CP, (NH 4 ) 3 [Zr(H 2/3 PO 4 ) 3 ] (ZrP), which consists of one-dimensional zirconium phosphate anionic chains and fully ordered charge-balancing NH 4 + cations. X-ray crystallography, neutron powder diffraction, and variable-temperature solid-state NMR spectroscopy suggest that protons are disordered within an inherent hydrogen-bonded infinite chain of acid-base pairs (N-H···O-P), leading to a stable anhydrous proton conductivity of 1.45 × 10 -3 S·cm -1 at 180 °C, one of the highest values among reported intermediate-temperature proton-conducting materials. First-principles and quantum molecular dynamics simulations were used to directly visualize the unique proton transport pathway involving very efficient proton exchange between NH 4 + and phosphate pairs, which is distinct from the common guest encapsulation/dehydration/superprotonic transition mechanisms. ZrP as the electrolyte was further assembled into a H 2 /O 2 fuel cell, which showed a record-high electrical power density of 12 mW·cm -2 at 180 °C among reported cells assembled from crystalline solid electrolytes, as well as a direct methanol fuel cell for the first time to demonstrate real applications. These cells were tested for over 15 h without notable power loss.

Protection of scaffold protein Isu from degradation by the Lon protease Pim1 as a component of Fe-S cluster biogenesis regulation.

PubMed

Ciesielski, Szymon J; Schilke, Brenda; Marszalek, Jaroslaw; Craig, Elizabeth A

2016-04-01

Iron-sulfur (Fe-S) clusters, essential protein cofactors, are assembled on the mitochondrial scaffold protein Isu and then transferred to recipient proteins via a multistep process in which Isu interacts sequentially with multiple protein factors. This pathway is in part regulated posttranslationally by modulation of the degradation of Isu, whose abundance increases >10-fold upon perturbation of the biogenesis process. We tested a model in which direct interaction with protein partners protects Isu from degradation by the mitochondrial Lon-type protease. Using purified components, we demonstrated that Isu is indeed a substrate of the Lon-type protease and that it is protected from degradation by Nfs1, the sulfur donor for Fe-S cluster assembly, as well as by Jac1, the J-protein Hsp70 cochaperone that functions in cluster transfer from Isu. Nfs1 and Jac1 variants known to be defective in interaction with Isu were also defective in protecting Isu from degradation. Furthermore, overproduction of Jac1 protected Isu from degradation in vivo, as did Nfs1. Taken together, our results lead to a model of dynamic interplay between a protease and protein factors throughout the Fe-S cluster assembly and transfer process, leading to up-regulation of Isu levels under conditions when Fe-S cluster biogenesis does not meet cellular demands. © 2016 Ciesielski et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

Long-Range Order in Nanocrystal Assemblies Determines Charge Transport of Films

DOE PAGES

Sainato, Michela; Shevitski, Brian; Sahu, Ayaskanta; ...

2017-07-18

Self-assembly of semiconductor nanocrystals (NCs) into two-dimensional patterns or three-dimensional (2- 3D) superstructures has emerged as a promising low-cost route to generate thin-film transistors and solar cells with superior charge transport because of enhanced electronic coupling between the NCs. Here, we show that lead sulfide (PbS) NCs solids featuring either short-range (disordered glassy solids, GSs) or long-range (superlattices, SLs) packing order are obtained solely by controlling deposition conditions of colloidal solution of NCs. In this study, we demonstrate the use of the evaporation-driven self-assembly method results in PbS NC SL structures that are observed over an area of 1 mmmore » × 100 μm, with long-range translational order of up to 100 nm. A number of ordered domains appear to have nucleated simultaneously and grown together over the whole area, imparting a polycrystalline texture to the 3D SL films. By contrast, a conventional, optimized spin-coating deposition method results in PbS NC glassy films with no translational symmetry and much shorter-range packing order in agreement with state-of-the-art reports. Further, we investigate the electronic properties of both SL and GS films, using a field-effect transistor configuration as a test platform. The long-range ordering of the PbS NCs into SLs leads to semiconducting NC-based solids, the mobility (μ) of which is 3 orders of magnitude higher than that of the disordered GSs. Furthemore, although spin-cast GSs of PbS NCs have weak ambipolar behavior with limited gate tunability, SLs of PbS NCs show a clear p-type behavior with significantly higher conductivities.« less

Uniform Test Assembly

ERIC Educational Resources Information Center

Belov, Dmitry I.

2008-01-01

In educational practice, a test assembly problem is formulated as a system of inequalities induced by test specifications. Each solution to the system is a test, represented by a 0-1 vector, where each element corresponds to an item included (1) or not included (0) into the test. Therefore, the size of a 0-1 vector equals the number of items "n"…

LPT. Shield test facility assembly and test building (TAN646), south ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LPT. Shield test facility assembly and test building (TAN-646), south facade. Camera facing north. High-bay section is pool room. Single-story section at right is control building (TAN-645). Small metal building is post-1970 addition. INEEL negative no. HD-40-7-3 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

The SLS Stages Intertank Structural Test Assembly (STA) arrives at MSFC

NASA Image and Video Library

2018-03-06

The SLS Stages Intertank Structural Test Assembly (STA) is rolling off the NASA Pegasus Barge at the MSFC Dock enroute to the MSFC 4619 Load Test Annex test facility for qualification testing. Members of MSFC Logistics Office and Move Team members gather for last minute instructions and safety briefing before off-loading STA hardware.

A Multiple Objective Test Assembly Approach for Exposure Control Problems in Computerized Adaptive Testing

ERIC Educational Resources Information Center

Veldkamp, Bernard P.; Verschoor, Angela J.; Eggen, Theo J. H. M.

2010-01-01

Overexposure and underexposure of items in the bank are serious problems in operational computerized adaptive testing (CAT) systems. These exposure problems might result in item compromise, or point at a waste of investments. The exposure control problem can be viewed as a test assembly problem with multiple objectives. Information in the test has…

Simulation numerique de l'effet du reflecteur radial sur les cellules rep en utilisant les codes DRAGON et DONJON

NASA Astrophysics Data System (ADS)

Bejaoui, Najoua

The pressurized water nuclear reactors (PWRs) is the largest fleet of nuclear reactors in operation around the world. Although these reactors have been studied extensively by designers and operators using efficient numerical methods, there are still some calculation weaknesses, given the geometric complexity of the core, still unresolved such as the analysis of the neutron flux's behavior at the core-reflector interface. The standard calculation scheme is a two steps process. In the first step, a detailed calculation at the assembly level with reflective boundary conditions, provides homogenized cross-sections for the assemblies, condensed to a reduced number of groups; this step is called the lattice calculation. The second step uses homogenized properties in each assemblies to calculate reactor properties at the core level. This step is called the full-core calculation or whole-core calculation. This decoupling of the two calculation steps is the origin of methodological bias particularly at the interface core reflector: the periodicity hypothesis used to calculate cross section librairies becomes less pertinent for assemblies that are adjacent to the reflector generally represented by these two models: thus the introduction of equivalent reflector or albedo matrices. The reflector helps to slowdown neutrons leaving the reactor and returning them to the core. This effect leads to two fission peaks in fuel assemblies localised at the core/reflector interface, the fission rate increasing due to the greater proportion of reentrant neutrons. This change in the neutron spectrum arises deep inside the fuel located on the outskirts of the core. To remedy this we simulated a peripheral assembly reflected with TMI-PWR reflector and developed an advanced calculation scheme that takes into account the environment of the peripheral assemblies and generate equivalent neutronic properties for the reflector. This scheme is tested on a core without control mechanisms and charged with fresh fuel. The results of this study showed that explicit representation of reflector and calculation of peripheral assembly with our advanced scheme allow corrections to the energy spectrum at the core interface and increase the peripheral power by up to 12% compared with that of the reference scheme.

Fibre optic sensor based measurements of flow-induced vibration in a liquid metal cooled nuclear reactor set-up

NASA Astrophysics Data System (ADS)

De Pauw, B.; Vanlanduit, S.; Van Tichelen, K.; Geernaert, T.; Thienpont, H.; Berghmans, F.

2017-04-01

Fuel assembly vibrations in nuclear reactor cores should not be excessive as these can compromise the lifetime of the assembly and lead to safety hazards. This issue is particularly relevant to new reactor designs that use liquid metal coolants. We therefore demonstrate accurate measurements of the vibrations of a fuel assembly in a lead-bismuth eutectic cooled installation with fibre Bragg grating (FBG) based sensors. The use of FBGs in combination with a dedicated sensor integration approach allows accounting for the severe geometrical constraints and providing for the required minimal intrusiveness of the instrumentation, identifying the vibration modes with required accuracy and observing the differences between the vibration amplitudes of the individual fuel pins as well as evidencing a low frequency fuel pin vibration mode resulting from the supports.

30 CFR 7.45 - Critical characteristics

Code of Federal Regulations, 2011 CFR

2011-07-01

... APPROVAL OF MINING PRODUCTS TESTING BY APPLICANT OR THIRD PARTY Battery Assemblies § 7.45 Critical characteristics The following critical characteristics shall be inspected or tested on each battery assembly to...

30 CFR 7.45 - Critical characteristics

Code of Federal Regulations, 2012 CFR

2012-07-01

... APPROVAL OF MINING PRODUCTS TESTING BY APPLICANT OR THIRD PARTY Battery Assemblies § 7.45 Critical characteristics The following critical characteristics shall be inspected or tested on each battery assembly to...

Soil pH mediates the balance between stochastic and deterministic assembly of bacteria

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tripathi, Binu M.; Stegen, James C.; Kim, Mincheol

Little is known about the factors affecting the relative influence of stochastic and deterministic processes that governs the assembly of microbial communities in successional soils. Here, we conducted a meta-analysis of bacterial communities using six different successional soils data sets, scattered across different regions, with different pH conditions in early and late successional soils. We found that soil pH was the best predictor of bacterial community assembly and the relative importance of stochastic and deterministic processes along successional soils. Extreme acidic or alkaline pH conditions lead to assembly of phylogenetically more clustered bacterial communities through deterministic processes, whereas pH conditionsmore » close to neutral lead to phylogenetically less clustered bacterial communities with more stochasticity. We suggest that the influence of pH, rather than successional age, is the main driving force in producing trends in phylogenetic assembly of bacteria, and that pH also influences the relative balance of stochastic and deterministic processes along successional soils. Given that pH had a much stronger association with community assembly than did successional age, we evaluated whether the inferred influence of pH was maintained when studying globally-distributed samples collected without regard for successional age. This dataset confirmed the strong influence of pH, suggesting that the influence of soil pH on community assembly processes occurs globally. Extreme pH conditions likely exert more stringent limits on survival and fitness, imposing strong selective pressures through ecological and evolutionary time. Taken together, these findings suggest that the degree to which stochastic vs. deterministic processes shape soil bacterial community assembly is a consequence of soil pH rather than successional age.« less

Sustained delivery of VEGF from designer self-assembling peptides improves cardiac function after myocardial infarction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guo, Hai-dong; Cui, Guo-hong; Yang, Jia-jun

Highlights: Black-Right-Pointing-Pointer The designer peptide LRKKLGKA could self-assemble into nanofibers. Black-Right-Pointing-Pointer Injection of LRKKLGKA peptides could promote the sustained delivery of VEGF. Black-Right-Pointing-Pointer Injection of VEGF with LRKKLGKA peptides lead to sufficient angiogenesis. Black-Right-Pointing-Pointer Injection of VEGF with LRKKLGKA peptides improves heart function. -- Abstract: Poor vascularization and insufficient oxygen supply are detrimental to the survival of residual cardiomyocytes or transplanted stem cells after myocardial infarction. To prolong and slow the release of angiogenic factors, which stimulate both angiogenesis and vasculogenesis, we constructed a novel self-assembling peptide by attaching the heparin-binding domain sequence LRKKLGKA to the self-assembling peptide RADA16. Thismore » designer self-assembling peptide self-assembled into nanofiber scaffolds under physiological conditions, as observed by atomic force microscopy. The injection of designer self-assembling peptides can efficiently provide the sustained delivery of VEGF for at least 1 month. At 4 weeks after transplantation, cardiac function was improved, and scar size and collagen deposition were markedly reduced in the group receiving VEGF with the LRKKLGKA scaffolds compared with groups receiving VEGF alone, LRKKLGKA scaffolds alone or VEGF with RADA16 scaffolds. The microvessel density in the VEGF with LRKKLGKA group was higher than that in the VEGF with RADA16 group. TUNEL and cleaved caspase-3 expression assays showed that the transplantation of VEGF with LRKKLGKA enhanced cell survival in the infarcted heart. These results present the tailor-made peptide scaffolds as a new generation of sustained-release biomimetic biomaterials and suggest that the use of angiogenic factors along with designer self-assembling peptides can lead to myocardial protection, sufficient angiogenesis, and improvement in cardiac function.« less

PBF Reactor Building (PER620). PBF crane holds fuel test assembly ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PBF Reactor Building (PER-620). PBF crane holds fuel test assembly aloft prior to lowering into reactor for test. Date: 1982. INEEL negative no. 82-4909 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

Engineering design of a high-temperature superconductor current lead

NASA Astrophysics Data System (ADS)

Niemann, R. C.; Cha, Y. S.; Hull, J. R.; Daugherty, M. A.; Buckles, W. E.

As part of the US Department of Energy's Superconductivity Pilot Center Program, Argonne National Laboratory and Superconductivity, Inc., are developing high-temperature superconductor (HTS) current leads suitable for application to superconducting magnetic energy storage systems. The principal objective of the development program is to design, construct, and evaluate the performance of HTS current leads suitable for near-term applications. Supporting objectives are to (1) develop performance criteria; (2) develop a detailed design; (3) analyze performance; (4) gain manufacturing experience in the areas of materials and components procurement, fabrication and assembly, quality assurance, and cost; (5) measure performance of critical components and the overall assembly; (6) identify design uncertainties and develop a program for their study; and (7) develop application-acceptance criteria.

KSC-04pd0388

NASA Image and Video Library

2004-03-05

KENNEDY SPACE CENTER, FLA. - At the SRB Assembly and Refurbishment Facility, STS-114 Mission Specialists Andrew Thomas (center) and Charles Camarda (right) look at a test panel of insulation material (left) cut in a liquid nitrogen process and a round aft heat seal (right) also treated in a liquid nitrogen process. At left is Mike Leppert, Manufacturing Operations project lead with United Space Alliance. The crew is at KSC for familiarization with Shuttle and mission equipment. The STS-114 mission is Logistics Flight 1, which is scheduled to deliver supplies and equipment, plus the external stowage platform, to the International Space Station.

LUNAR RECEIVING LABORATORY (LRL) - CLARK, ROBERT, DR. - JSC

NASA Image and Video Library

1973-11-05

S73-36161 (November 1973) --- In the Radiation Counting Laboratory sixty feet underground at JSC, Dr. Robert S. Clark prepares to load pieces of iridium foil -- sandwiched between plastic sheets -- into the laboratory's radiation detector. The iridium foil strips were worn by the crew of the second Skylab flight in personal radiation dosimeters throughout their 59 1/2 days in space. Inside the radiation detector assembly surrounded by 28 tons of lead shielding, the sample will be tested to determine the total neutron dose to which the astronauts were exposed during their long stay aboard the space station. Photo credit: NASA

Hybrid Wing Body Multi-Bay Test Article Analysis and Assembly Final Report

NASA Technical Reports Server (NTRS)

Velicki, Alexander; Hoffman, Krishna; Linton, Kim A.; Baraja, Jaime; Wu, Hsi-Yung T.; Thrash, Patrick

2017-01-01

This report summarizes work performed by The Boeing Company, through its Boeing Research & Technology organization located in Huntington Beach, California, under the Environmentally Responsible Aviation (ERA) project. The report documents work performed to structurally analyze and assemble a large-scale Multi-bay Box (MBB) Test Article capable of withstanding bending and internal pressure loadings representative of a Hybrid Wing Body (HWB) aircraft. The work included fabrication of tooling elements for use in the fabrication and assembly of the test article.

Functions of Ribosomal Proteins in Assembly of Eukaryotic Ribosomes In Vivo

PubMed Central

2016-01-01

The proteome of cells is synthesized by ribosomes, complex ribonucleoproteins that in eukaryotes contain 79–80 proteins and four ribosomal RNAs (rRNAs) more than 5,400 nucleotides long. How these molecules assemble together and how their assembly is regulated in concert with the growth and proliferation of cells remain important unanswered questions. Here, we review recently emerging principles to understand how eukaryotic ribosomal proteins drive ribosome assembly in vivo. Most ribosomal proteins assemble with rRNA cotranscriptionally; their association with nascent particles is strengthened as assembly proceeds. Each subunit is assembled hierarchically by sequential stabilization of their subdomains. The active sites of both subunits are constructed last, perhaps to prevent premature engagement of immature ribosomes with active subunits. Late-assembly intermediates undergo quality-control checks for proper function. Mutations in ribosomal proteins that affect mostly late steps lead to ribosomopathies, diseases that include a spectrum of cell type–specific disorders that often transition from hypoproliferative to hyperproliferative growth. PMID:25706898

DOE Office of Scientific and Technical Information (OSTI.GOV)

West, W.S.

Progress during the period includes completion of the SNAP 7C system tests, completion of safety analysis for the SNAP 7A and C systems, assembly and initial testing of SNAP 7A, assembly of a modified reliability model, and assembly of a 10-W generator. Other activities include completion of thermal and safety analyses for SNAP 7B and D generators and fuel processing for these generators. (J.R.D.)

Design and Fabrication of Cryostat Interface and Electronics for High Performance Antimatter Trap (HI-PAT)

NASA Technical Reports Server (NTRS)

Smith, Gerald A.

1999-01-01

Included in Appendix I to this report is a complete set of design and assembly schematics for the high vacuum inner trap assembly, cryostat interfaces and electronic components for the MSFC HI-PAT. Also included in the final report are summaries of vacuum tests, and electronic tests performed upon completion of the assembly.

Architectured Materials to Improve the Reliability of Power Electronics Modules: Substrate and Lead-Free Solder

NASA Astrophysics Data System (ADS)

Kaabi, Abderrahmen; Bienvenu, Yves; Ryckelynck, David; Pierre, Bertrand

2014-03-01

Power electronics modules (>100 A, >500 V) are essential components for the development of electrical and hybrid vehicles. These modules are formed from silicon chips (transistors and diodes) assembled on copper substrates by soldering. Owing to the fact that the assembly is heterogeneous, and because of thermal gradients, shear stresses are generated in the solders and cause premature damage to such electronics modules. This work focuses on architectured materials for the substrate and on lead-free solders to reduce the mechanical effects of differential expansion, improve the reliability of the assembly, and achieve a suitable operating temperature (<175°C). These materials are composites whose thermomechanical properties have been optimized by numerical simulation and validated experimentally. The substrates have good thermal conductivity (>280 W m-1 K-1) and a macroscopic coefficient of thermal expansion intermediate between those of Cu and Si, as well as limited structural evolution in service conditions. An approach combining design, optimization, and manufacturing of new materials has been followed in this study, leading to improved thermal cycling behavior of the component.

A Design Problem of Assembly Line Systems using Genetic Algorithm under the BTO Environment

NASA Astrophysics Data System (ADS)

Abe, Kazuaki; Yamada, Tetsuo; Matsui, Masayuki

Under the BTO environment, stochastic assembly lines require design methods which shorten not only the production lead time but also the ready time for the line design. We propose a design method for Assembly Line Systems (ALS) in Yamada et al. (2001) by using Genetic Algorithm (GA) and Adam-Eve GA, in which all design variables are determined in consideration of constraints such as line length related to the production lead time. First, an ALS model with a line length constraint is introduced, and an optimal design problem is set to maximize the net reward under shorter lead time. Next, a simulation optimization method is developed using Adam-Eve GA and traditional GA. Finally, an optimal design example is shown and discussed by comparing the 2-stage design by Yamada et al. (2001) and both the GA designs. It is shown that the Adam-Eve GA is superior to the traditional GA design in terms of computational time though there is only a slight difference in terms of net reward.

Dissection of molecular assembly dynamics by tracking orientation and position of single molecules in live cells

PubMed Central

McQuilken, Molly; La Riviere, Patrick J.; Occhipinti, Patricia; Verma, Amitabh; Oldenbourg, Rudolf; Gladfelter, Amy S.; Tani, Tomomi

2016-01-01

Regulation of order, such as orientation and conformation, drives the function of most molecular assemblies in living cells but remains difficult to measure accurately through space and time. We built an instantaneous fluorescence polarization microscope, which simultaneously images position and orientation of fluorophores in living cells with single-molecule sensitivity and a time resolution of 100 ms. We developed image acquisition and analysis methods to track single particles that interact with higher-order assemblies of molecules. We tracked the fluctuations in position and orientation of molecules from the level of an ensemble of fluorophores down to single fluorophores. We tested our system in vitro using fluorescently labeled DNA and F-actin, in which the ensemble orientation of polarized fluorescence is known. We then tracked the orientation of sparsely labeled F-actin network at the leading edge of migrating human keratinocytes, revealing the anisotropic distribution of actin filaments relative to the local retrograde flow of the F-actin network. Additionally, we analyzed the position and orientation of septin-GFP molecules incorporated in septin bundles in growing hyphae of a filamentous fungus. Our data indicate that septin-GFP molecules undergo positional fluctuations within ∼350 nm of the binding site and angular fluctuations within ∼30° of the central orientation of the bundle. By reporting position and orientation of molecules while they form dynamic higher-order structures, our approach can provide insights into how micrometer-scale ordered assemblies emerge from nanoscale molecules in living cells. PMID:27679846

Self-assembled thin films of Fe3O4-Ag composite nanoparticles for spintronic applications

NASA Astrophysics Data System (ADS)

Jiang, Chengpeng; Leung, Chi Wah; Pong, Philip W. T.

2017-10-01

Controlled self-assembly of multi-component magnetic nanoparticles could lead to nanomaterial-based magnetic devices with novel structures and intriguing properties. Herein, self-assembled thin films of Fe3O4-Ag composite nanoparticles (CNPs) with hetero-dimeric shapes were fabricated using interfacial assembly method. The CNP-assembled thin films were further transferred to patterned silicon substrates followed by vacuum annealing, producing CNP-based magnetoresistive (MR) devices. Due to the presence of intra-particle interfaces and inter-particle barriers, an enhanced MR ratio and a non-linear current-voltage relation were observed in the device. The results of this work can potentially pave the way to the future exploration and development of spintronic devices built from composite nanomaterials.

40 CFR 1054.305 - How must I prepare and test my production-line engines?

Code of Federal Regulations, 2011 CFR

2011-07-01

... way that represents the assembly procedures for other engines in the engine family. You must ask us to approve any deviations from your normal assembly procedures for other production engines in the engine family. (a) Test procedures. Test your production-line engines using the applicable testing procedures in...

40 CFR 1054.305 - How must I prepare and test my production-line engines?

Code of Federal Regulations, 2013 CFR

2013-07-01

... way that represents the assembly procedures for other engines in the engine family. You must ask us to approve any deviations from your normal assembly procedures for other production engines in the engine family. (a) Test procedures. Test your production-line engines using the applicable testing procedures in...

40 CFR 1054.305 - How must I prepare and test my production-line engines?

Code of Federal Regulations, 2010 CFR

2010-07-01

... way that represents the assembly procedures for other engines in the engine family. You must ask us to approve any deviations from your normal assembly procedures for other production engines in the engine family. (a) Test procedures. Test your production-line engines using the applicable testing procedures in...

40 CFR 1054.305 - How must I prepare and test my production-line engines?

Code of Federal Regulations, 2014 CFR

2014-07-01

... way that represents the assembly procedures for other engines in the engine family. You must ask us to approve any deviations from your normal assembly procedures for other production engines in the engine family. (a) Test procedures. Test your production-line engines using the applicable testing procedures in...

40 CFR 1054.305 - How must I prepare and test my production-line engines?

Code of Federal Regulations, 2012 CFR

2012-07-01

... way that represents the assembly procedures for other engines in the engine family. You must ask us to approve any deviations from your normal assembly procedures for other production engines in the engine family. (a) Test procedures. Test your production-line engines using the applicable testing procedures in...

Observed-Score Equating as a Test Assembly Problem.

ERIC Educational Resources Information Center

van der Linden, Wim J.; Luecht, Richard M.

1998-01-01

Derives a set of linear conditions of item-response functions that guarantees identical observed-score distributions on two test forms. The conditions can be added as constraints to a linear programming model for test assembly. An example illustrates the use of the model for an item pool from the Law School Admissions Test (LSAT). (SLD)

Computer Assisted Assembly of Tests at Educational Testing Service.

ERIC Educational Resources Information Center

Educational Testing Service, Princeton, NJ.

Two basic requirements for the successful initiation of a program for test assembly are the development of detailed item content classification systems and the delineation of the professional judgements made in building a test from a pool of items to detailed content, ability, and statistical specifications in terms precise enough to be translated…

Integrated Advanced Microwave Sounding Unit-A (AMSU-A). Performance Verification Report: METSAT Phase Locked Oscillator Assembly, P/N 1334360-1, S/N's F03 and F04

NASA Technical Reports Server (NTRS)

Pines, D.

1998-01-01

Two Flight Model AMSU-A Phase Locked Oscillators (P/N 1348360-1, S/Ns F03 and F04) have been tested per AES Test Procedure AE-26758. The tests included vibration testing, thermal cycle testing, AM/FM Noise testing, and full functional testing. EMI/REO 2 Testing was not performed. (See test data for S/N F01). Both AMSU-A Phase Locked Oscillators satisfactorily passed all performance requirements of the AE-26633 Product specification. During thermal cycling of PLO serial number F03, the oven and data logger momentarily lost power, including a loss of data. The unit did not experience any thermal stress. TAR 003134 describes the corrective action. Prior to testing PLO serial number FO4, power was applied to the unit. (+15v,-15v) the unit did not display the proper phase lock. Upon test equipment check out a connector was found to be defective. TAR 003133 describes the corrective action. After completion of testing of PLO serial number F04 was installed into Receiver Assembly F02. Upon testing F02 Receiver Assembly the unit was found not to phase lock at ambient temperature. Removal of PLO Assembly F04 was required. R2 was the real issue. Solithane was secondary. Troubleshooting revealed excessive solithane on inner PLL Assembly cover inhibiting optimum grounding. Also, R2 was reselected which increased the lock range from -30 C to +60 C. TAR 002737 describes the corrective action.

Rotor blade assembly having internal loading features

DOE Office of Scientific and Technical Information (OSTI.GOV)

Soloway, Daniel David

Rotor blade assemblies and wind turbines are provided. A rotor blade assembly includes a rotor blade having exterior surfaces defining a pressure side, a suction side, a leading edge and a trailing edge each extending between a tip and a root, the rotor blade defining a span and a chord, the exterior surfaces defining an interior of the rotor blade. The rotor blade assembly further includes a loading assembly, the loading assembly including a weight disposed within the interior and movable generally along the span of the rotor blade, the weight connected to a rotor blade component such that movementmore » of the weight towards the tip causes application of a force to the rotor blade component by the weight. Centrifugal force due to rotation of the rotor blade biases the weight towards the tip.« less

49 CFR 572.5 - General description.

Code of Federal Regulations, 2010 CFR

2010-10-01

... M080—Right leg assembly SA 150 M081—Left leg assembly SA 150 M010—Head assembly SA 150 M020—Neck... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) ANTHROPOMORPHIC TEST DEVICES 50th Percentile Male § 572... grouped by component assemblies under the following nine headings: SA 150 M070—Right arm assembly SA 150...

Cryogenic Fiber Optic Assemblies for Spaceflight Environments: Design, Manufacturing, Testing, and Integration

NASA Technical Reports Server (NTRS)

Thomes, W. Joe; Ott, Melanie N.; Chuska, Richard; Switzer, Robert; Onuma, Eleanya; Blair, Diana; Frese, Erich; Matyseck, Marc

2016-01-01

Fiber optic assemblies have been used on spaceflight missions for many years as an enabling technology for routing, transmitting, and detecting optical signals. Due to the overwhelming success of NASA in implementing fiber optic assemblies on spaceflight science-based instruments, system scientists increasingly request fibers that perform in extreme environments while still maintaining very high optical transmission, stability, and reliability. Many new applications require fiber optic assemblies that will operate down to cryogenic temperatures as low as 20 Kelvin. In order for the fiber assemblies to operate with little loss in optical throughput at these extreme temperatures requires a system level approach all the way from how the fiber assembly is manufactured to how it is held, routed, and integrated. The NASA Goddard Code 562 Photonics Group has been designing, manufacturing, testing, and integrating fiber optics for spaceflight and other high reliability applications for nearly 20 years. Design techniques and lessons learned over the years are consistently applied to developing new fiber optic assemblies that meet these demanding environments. System level trades, fiber assembly design methods, manufacturing, testing, and integration will be discussed. Specific recent examples of ground support equipment for the James Webb Space Telescope (JWST); the Ice, Cloud and Land Elevation Satellite-2 (ICESat-2); and others will be included.

Cryogenic fiber optic assemblies for spaceflight environments: design, manufacturing, testing, and integration

NASA Astrophysics Data System (ADS)

Thomes, W. Joe; Ott, Melanie N.; Chuska, Richard; Switzer, Robert; Onuma, Eleanya; Blair, Diana; Frese, Erich; Matyseck, Marc

2016-09-01

Fiber optic assemblies have been used on spaceflight missions for many years as an enabling technology for routing, transmitting, and detecting optical signals. Due to the overwhelming success of NASA in implementing fiber optic assemblies on spaceflight science-based instruments, system scientists increasingly request fibers that perform in extreme environments while still maintaining very high optical transmission, stability, and reliability. Many new applications require fiber optic assemblies that will operate down to cryogenic temperatures as low as 20 Kelvin. In order for the fiber assemblies to operate with little loss in optical throughput at these extreme temperatures requires a system level approach all the way from how the fiber assembly is manufactured to how it is held, routed, and integrated. The NASA Goddard Code 562 Photonics Group has been designing, manufacturing, testing, and integrating fiber optics for spaceflight and other high reliability applications for nearly 20 years. Design techniques and lessons learned over the years are consistently applied to developing new fiber optic assemblies that meet these demanding environments. System level trades, fiber assembly design methods, manufacturing, testing, and integration will be discussed. Specific recent examples of ground support equipment for the James Webb Space Telescope (JWST); the Ice, Cloud and Land Elevation Satellite-2 (ICESat- 2); and others will be included.

W18O49 nanowires assembled on carbon felt for application to supercapacitors

NASA Astrophysics Data System (ADS)

Jung, Jinjoo; Kim, Do Hyung

2018-03-01

For supercapacitor applications, W18O49 nanowires have been extensively grown on graphitic carbon felt using a facile solvothermal method. The diameter and length of the nanowires are about 7 and 300 nm, respectively. The nanowires consist of monoclinic W18O49 grown along the [010] direction, as shown by TEM and XRD analyses. The W18O49 nanowires, assembled on carbon felt, exhibit a high capacity of 588.33 F/g at a current density of 1 A/g together with an excellent cycle performance, and a low internal resistance during the electrochemical tests. This outstanding performance may originate from the three-dimensional porous nanostructure of these W18O49 nanowires, which leads to a reduction in the resistance and fast reaction kinetics due to the high specific surface area and electrolyte accessibility. Furthermore, sufficient oxygen deficiencies of the substoichiometric tungsten oxide can also contribute to the electrochemical activity, which can be confirmed by comparison of CV and EIS data with WO3 nanowires.

Differences in gamma frequencies across visual cortex restrict their possible use in computation.

PubMed

Ray, Supratim; Maunsell, John H R

2010-09-09

Neuronal oscillations in the gamma band (30-80 Hz) have been suggested to play a central role in feature binding or establishing channels for neural communication. For these functions, the gamma rhythm frequency must be consistent across neural assemblies encoding the features of a stimulus. Here we test the dependence of gamma frequency on stimulus contrast in V1 cortex of awake behaving macaques and show that gamma frequency increases monotonically with contrast. Changes in stimulus contrast over time leads to a reliable gamma frequency modulation on a fast timescale. Further, large stimuli whose contrast varies across space generate gamma rhythms at significantly different frequencies in simultaneously recorded neuronal assemblies separated by as little as 400 microm, making the gamma rhythm a poor candidate for binding or communication, at least in V1. Instead, our results suggest that the gamma rhythm arises from local interactions between excitation and inhibition. 2010 Elsevier Inc. All rights reserved.

Fine pitch thermosonic wire bonding: analysis of state-of-the-art manufacturing capability

NASA Astrophysics Data System (ADS)

Cavasin, Daniel

1995-09-01

A comprehensive process characterization was performed at the Motorola plastic package assembly site in Selangor, Malaysia, to document the current fine pitch wire bond process capability, using state-of-the-art equipment, in an actual manufacturing environment. Two machines, representing the latest technology from two separate manufacturers, were operated one shift per day for five days, bonding a 132 lead Plastic Quad Flat Pack. Using a test device specifically designed for fine pitch wire bonding, the bonding programs were alternated between 107 micrometers and 92 micrometers pad pitch, running each pitch for a total of 1600 units per machine. Wire, capillary type, and related materials were standardized and commercially available. A video metrology measurement system, with a demonstrated six sigma repeatability band width of 0.51 micrometers , was utilized to measure the bonded units for bond dimensions and placement. Standard Quality Assurance (QA) metrics were also performed. Results indicate that state-of-the-art thermosonic wire bonding can achieve acceptable assembly yields at these fine pad pitches.

James Webb Space Telescope's Golden Mirror Unveiled

NASA Image and Video Library

2017-12-08

NASA engineers unveil the giant golden mirror of NASA's James Webb Space Telescope, and it's goldenly delicious! The 18 mirrors that make up the primary mirror were individually protected with a black covers when they were assembled on the telescope structure. Now, for the first time since the primary mirror was completed, the covers have been lifted. Standing tall and glimmering gold inside NASA's Goddard Space Flight Center's clean room in Greenbelt, Maryland, this mirror will be the largest yet sent into space. Currently, engineers are busy assembling and testing the other pieces of the telescope. Read more: go.nasa.gov/1TejHg4 Credit: NASA/Goddard/Chris Gunn NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram

Hantavirus Gn and Gc Glycoproteins Self-Assemble into Virus-Like Particles

PubMed Central

Acuña, Rodrigo; Cifuentes-Muñoz, Nicolás; Márquez, Chantal L.; Bulling, Manuela; Klingström, Jonas; Mancini, Roberta; Lozach, Pierre-Yves

2014-01-01

How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera. PMID:24335294

Light driven mesoscale assembly of a coordination polymeric gelator into flowers and stars with distinct properties.

PubMed

Mukhopadhyay, Rahul Dev; Praveen, Vakayil K; Hazra, Arpan; Maji, Tapas Kumar; Ajayaghosh, Ayyappanpillai

2015-11-13

Control over the self-assembly process of porous organic-inorganic hybrids often leads to unprecedented polymorphism and properties. Herein we demonstrate how light can be a powerful tool to intervene in the kinetically controlled mesoscale self-assembly of a coordination polymeric gelator. Ultraviolet light induced coordination modulation via photoisomerisation of an azobenzene based dicarboxylate linker followed by aggregation mediated crystal growth resulted in two distinct morphological forms (flowers and stars), which show subtle differences in their physical properties.

Mars pathfinder Rover egress deployable ramp assembly

NASA Technical Reports Server (NTRS)

Spence, Brian R.; Sword, Lee F.

1996-01-01

The Mars Pathfinder Program is a NASA Discovery Mission, led by the Jet Propulsion Laboratory, to launch and place a small planetary Rover for exploration on the Martian surface. To enable safe and successful egress of the Rover vehicle from the spacecraft, a pair of flight-qualified, deployable ramp assemblies have been developed. This paper focuses on the unique, lightweight deployable ramp assemblies. A brief mission overview and key design requirements are discussed. Design and development activities leading to qualification and flight systems are presented.

Two-Dimensional Holey Nanoarchitectures Created by Confined Self-Assembly of Nanoparticles via Block Copolymers: From Synthesis to Energy Storage Property

DOE Office of Scientific and Technical Information (OSTI.GOV)

Peng, Lele; Fang, Zhiwei; Li, Jing

Advances in liquid-phase exfoliation and surfactant-directed anisotropic growth of two-dimensional (2D) nanosheets have enabled their rapid development. However, it remains challenging to develop assembly strategies that lead to the construction of 2D nanomaterials with well-defined geometry and functional nanoarchitectures that are tailored to specific applications. Here we report a facile self-assembly method leading to the controlled synthesis of 2D transition metal oxide (TMO) nanosheets containing a high density of holes. We utilize graphene oxide sheets as a sacrificial template and Pluronic copolymers as surfactant. By using ZnFe 2O 4 (ZFO) nanoparticles as a model material, we demonstrate that by tuningmore » the molecular weight of the Pluronic copolymers that we can incorporate the ZFO particles and tune the size of the holes in the sheets. The resulting 2D ZFO nanosheets offer synergistic characteristics including increased electrochemically active surface areas, shortened ion diffusion paths, and strong inherent mechanical properties, leading to enhanced lithium-ion storage properties. Post-cycling characterization confirms that the samples maintain structural integrity after electrochemical cycling. In conclusion, our findings demonstrate that this template-assisted self-assembly method is a useful bottom-up route for controlled synthesis of 2D nanoarchitectures, and these holey 2D nanoarchitectures are promising for improving the electrochemical performance of nextgeneration lithium-ion batteries.« less

Two-Dimensional Holey Nanoarchitectures Created by Confined Self-Assembly of Nanoparticles via Block Copolymers: From Synthesis to Energy Storage Property

DOE PAGES

Peng, Lele; Fang, Zhiwei; Li, Jing; ...

2017-12-20

Advances in liquid-phase exfoliation and surfactant-directed anisotropic growth of two-dimensional (2D) nanosheets have enabled their rapid development. However, it remains challenging to develop assembly strategies that lead to the construction of 2D nanomaterials with well-defined geometry and functional nanoarchitectures that are tailored to specific applications. Here we report a facile self-assembly method leading to the controlled synthesis of 2D transition metal oxide (TMO) nanosheets containing a high density of holes. We utilize graphene oxide sheets as a sacrificial template and Pluronic copolymers as surfactant. By using ZnFe 2O 4 (ZFO) nanoparticles as a model material, we demonstrate that by tuningmore » the molecular weight of the Pluronic copolymers that we can incorporate the ZFO particles and tune the size of the holes in the sheets. The resulting 2D ZFO nanosheets offer synergistic characteristics including increased electrochemically active surface areas, shortened ion diffusion paths, and strong inherent mechanical properties, leading to enhanced lithium-ion storage properties. Post-cycling characterization confirms that the samples maintain structural integrity after electrochemical cycling. In conclusion, our findings demonstrate that this template-assisted self-assembly method is a useful bottom-up route for controlled synthesis of 2D nanoarchitectures, and these holey 2D nanoarchitectures are promising for improving the electrochemical performance of nextgeneration lithium-ion batteries.« less

14 CFR 33.88 - Engine overtemperature test.

Code of Federal Regulations, 2011 CFR

2011-01-01

... AIRWORTHINESS STANDARDS: AIRCRAFT ENGINES Block Tests; Turbine Aircraft Engines § 33.88 Engine overtemperature... this run, the turbine assembly must be within serviceable limits. (b) In addition to the test... this run, the turbine assembly may exhibit distress beyond the limits for an overtemperature condition...

14 CFR 33.88 - Engine overtemperature test.

Code of Federal Regulations, 2013 CFR

2013-01-01

... AIRWORTHINESS STANDARDS: AIRCRAFT ENGINES Block Tests; Turbine Aircraft Engines § 33.88 Engine overtemperature... this run, the turbine assembly must be within serviceable limits. (b) In addition to the test... this run, the turbine assembly may exhibit distress beyond the limits for an overtemperature condition...

14 CFR 33.88 - Engine overtemperature test.

Code of Federal Regulations, 2014 CFR

2014-01-01

... AIRWORTHINESS STANDARDS: AIRCRAFT ENGINES Block Tests; Turbine Aircraft Engines § 33.88 Engine overtemperature... this run, the turbine assembly must be within serviceable limits. (b) In addition to the test... this run, the turbine assembly may exhibit distress beyond the limits for an overtemperature condition...

Fabrication Control Plan for ORNL RH-LOCA ATF Test Specimens to be Irradiated in the ATR

DOE Office of Scientific and Technical Information (OSTI.GOV)

Field, Kevin G.; Howard, Richard; Teague, Michael

2014-06-01

The purpose of this fabrication plan is (1) to summarize the design of a set of rodlets that will be fabricated and then irradiated in the Advanced Test Reactor (ATR) and (2) provide requirements for fabrication and acceptance criteria for inspections of the Light Water Reactor (LWR) – Accident Tolerant Fuels (ATF) rodlet components. The functional and operational (F&OR) requirements for the ATF program are identified in the ATF Test Plan. The scope of this document only covers fabrication and inspections of rodlet components detailed in drawings 604496 and 604497. It does not cover the assembly of these items tomore » form a completed test irradiation assembly or the inspection of the final assembly, which will be included in a separate INL final test assembly specification/inspection document. The controls support the requirements that the test irradiations must be performed safely and that subsequent examinations must provide valid results.« less

SP-100 GES/NAT radiation shielding systems design and development testing

NASA Astrophysics Data System (ADS)

Disney, Richard K.; Kulikowski, Henry D.; McGinnis, Cynthia A.; Reese, James C.; Thomas, Kevin; Wiltshire, Frank

1991-01-01

Advanced Energy Systems (AES) of Westinghouse Electric Corporation is under subcontract to the General Electric Company to supply nuclear radiation shielding components for the SP-100 Ground Engineering System (GES) Nuclear Assembly Test to be conducted at Westinghouse Hanford Company at Richland, Washington. The radiation shielding components are integral to the Nuclear Assembly Test (NAT) assembly and include prototypic and non-prototypic radiation shielding components which provide prototypic test conditions for the SP-100 reactor subsystem and reactor control subsystem components during the GES/NAT operations. W-AES is designing three radiation shield components for the NAT assembly; a prototypic Generic Flight System (GFS) shield, the Lower Internal Facility Shield (LIFS), and the Upper Internal Facility Shield (UIFS). This paper describes the design approach and development testing to support the design, fabrication, and assembly of these three shield components for use within the vacuum vessel of the GES/NAT. The GES/NAT shields must be designed to operate in a high vacuum which simulates space operations. The GFS shield and LIFS must provide prototypic radiation/thermal environments and mechanical interfaces for reactor system components. The NAT shields, in combination with the test facility shielding, must provide adequate radiation attenuation for overall test operations. Special design considerations account for the ground test facility effects on the prototypic GFS shield. Validation of the GFS shield design and performance will be based on detailed Monte Carlo analyses and developmental testing of design features. Full scale prototype testing of the shield subsystems is not planned.

Solving Infeasibility Problems in Computerized Test Assembly.

ERIC Educational Resources Information Center

Timminga, Ellen

1998-01-01

Discusses problems of diagnosing and repairing infeasible linear-programming models in computerized test assembly. Demonstrates that it is possible to localize the causes of infeasibility, although this is not always easy. (SLD)

The MARVEL assembly for neutron multiplication.

PubMed

Chichester, David L; Kinlaw, Mathew T

2013-10-01

A new multiplying test assembly is under development at Idaho National Laboratory to support research, validation, evaluation, and learning. The item is comprised of three stacked, highly-enriched uranium (HEU) cylinders, each 11.4 cm in diameter and having a combined height of up to 11.7 cm. The combined mass of all three cylinders is 20.3 kg of HEU. Calculations for the bare configuration of the assembly indicate a multiplication level of >3.5 (k(eff)=0.72). Reflected configurations of the assembly, using either polyethylene or tungsten, are possible and have the capability of raising the assembly's multiplication level to greater than 10. This paper describes simulations performed to assess the assembly's multiplication level under different conditions and describes the resources available at INL to support the use of these materials. We also describe some preliminary calculations and test activities using the assembly to study neutron multiplication. Copyright © 2013 Elsevier Ltd. All rights reserved.

Molecular Self-Assembly of Short Aromatic Peptides: From Biology to Nanotechnology and Material Science

NASA Astrophysics Data System (ADS)

Gazit, Ehud

2013-03-01

The formation of ordered amyloid fibrils is the hallmark of several diseases of unrelated origin. In spite of grave clinical consequence, the mechanism of amyloid formation is not fully understood. We have suggested, based on experimental and bioinformatic analysis, that aromatic interactions may provide energetic contribution as well as order and directionality in the molecular-recognition and self-association processes that lead to the formation of these assemblies. This is in line with the well-known central role of aromatic-stacking interactions in self-assembly processes. Our works on the mechanism of aromatic peptide self-assembly, lead to the discovery that the diphenylalanine recognition motif self-assembles into peptide nanotubes with a remarkable persistence length. Other aromatic homodipeptides could self-assemble in nano-spheres, nano-plates, nano-fibrils and hydrogels with nano-scale order. We demonstrated that the peptide nanostructures have unique chemical, physical and mechanical properties including ultra-rigidity as aramides, semi-conductive, piezoelectric and non-linear optic properties. We also demonstrated the ability to use these peptide nanostructures as casting mold for the fabrication of metallic nano-wires and coaxial nano-cables. The application of the nanostructures was demonstrated in various fields including electrochemical biosensors, tissue engineering, and molecular imaging. Finally, we had developed ways for depositing of the peptide nanostructures and their organization. We had use inkjet technology as well as vapour deposition methods to coat surface and from the peptide ``nano-forests''. We recently demonstrated that even a single phenylalanine amino-acid can form well-ordered fibrilar assemblies.

Integrated testing strategies can be optimal for chemical risk classification.

PubMed

Raseta, Marko; Pitchford, Jon; Cussens, James; Doe, John

2017-08-01

There is an urgent need to refine strategies for testing the safety of chemical compounds. This need arises both from the financial and ethical costs of animal tests, but also from the opportunities presented by new in-vitro and in-silico alternatives. Here we explore the mathematical theory underpinning the formulation of optimal testing strategies in toxicology. We show how the costs and imprecisions of the various tests, and the variability in exposures and responses of individuals, can be assembled rationally to form a Markov Decision Problem. We compute the corresponding optimal policies using well developed theory based on Dynamic Programming, thereby identifying and overcoming some methodological and logical inconsistencies which may exist in the current toxicological testing. By illustrating our methods for two simple but readily generalisable examples we show how so-called integrated testing strategies, where information of different precisions from different sources is combined and where different initial test outcomes lead to different sets of future tests, can arise naturally as optimal policies. Copyright © 2017 Elsevier Inc. All rights reserved.

Design, assembly, and metrology of an oil-immersion microscope objective with long working distance

NASA Astrophysics Data System (ADS)

Peng, Wei-Jei; Lin, Wen-Lung; Kuo, Hui-Jean; Ho, Cheng-Fang; Hsu, Wei-Yao

2016-10-01

The design, tolerance sensitivity reduction, assembly, and optical bench test for an oil-immersion microscope objective with long working distance employed in a lattice light-sheet microscope is presented in this paper. In this application, the orthogonal excitation and detection objectives are dipped in an oil medium. The excitation objective focuses the incident laser beam to generate fluorescence on specimen for collecting by detection objective. The excitation objective is custom-designed to meet the requirement specification such as oil-immersion, the long working distance, and numerical aperture (NA) of 0.5, etc. To produce an acceptable point spread function (PSF) for effective excitation, the performance of the objective needs to be close to diffraction limit. Because the tolerance of the modulation transfer function (MTF) is more and more sensitive at higher spatial frequency, it is extremely critical to keep the performance after manufacture. Consequently, an insensitive optical design is very important for relaxing tolerance. We compare the design with and without tolerance sensitivity reduction, and the as-built MTF shows the result. Furthermore, the method for sensitivity reduction is presented. The opto-mechanical design and assembly method are also discussed. Eventually, the objective with five spherical lenses was fabricated. In optical bench test, the depth of the oil is sensitive to MTF, and it leads to the complicated adjustment. For solving this issue, we made an index-matching lens to replace oil for measurement easily. Finally, the measured MTF of the excitation objective can accomplish the requirement specification and successfully employed in a lattice light-sheet microscope.

The Potential of Self-assembling Peptides for Enhancement of In Vitro Remineralisation of White Spot Lesions as Measured by Quantitative Laser Fluorescence.

PubMed

Golland, Luca; Schmidlin, Patrick R; Schätzle, Marc

To test the remineralisation potential of a single application of self-assembling peptides or acidic fluoride solution using quantitative light-induced fluorescence (QLF) in vitro. Bovine enamel disks were prepared, and white spot lesions were created on one half of the disk with an acidic buffer solution. After demineralisation, disks were allocated into three groups of 11 specimens each. Group A served as a control group and received no treatment. Group B had a single application of fluoride, and group C was treated once with self-assembling peptides. All disks were embedded in a plastic mold (diameter 15 mm, height 9 mm) with an a-silicone, and remineralisation was initiated using a pH-cycling protocol for five days. Four experimental regions on each disk were measured prior to the start of the study (T0), after demineralisation (T1) and after the remineralisation process (T2) using QLF. After demineralisation, all areas showed a distinct loss of fluorescence, with no statistically significant difference between the groups (ΔF from -69.3 to -10.2). After remineralisation, samples of group B (treated with fluoride) showed a statistically significant fluorescence increase (ΔF from T1 to T2 15.2 ± 7.3) indicating remineralisation, whereas the samples of control group A and group C (treated with self-assembling peptides) showed no significant changes in ΔF of 1.1 ± 1.9 and 2.5 ± 1.9, respectively. Application of self-assembling peptides on demineralised bovine enamel did not lead to increased fluorescence using QLF, indicating either lack of remineralisation or irregular crystals. Increased fluorescence using QLF indicated mineral gain following a single application of a highly concentrated fluoride.

Theranostic self-assembly structure of gold nanoparticles for NIR photothermal therapy and X-Ray computed tomography imaging.

PubMed

Deng, Heng; Zhong, Yanqi; Du, Meihong; Liu, Qinjun; Fan, Zhanming; Dai, Fengying; Zhang, Xin

2014-01-01

The controllable self-assembly of amphiphilic mixed polymers grafted gold nanoparitcles (AuNPs) leads to strong interparticle plasmonic coupling, which can be tuned to the near-infrared (NIR) region for enhanced photothermal therapy (PTT). In this study, an improved thiolation method was adopted for ATRP and ROP polymer to obtain amphiphilic brushes of PMEO2MA-SH and PCL-SH. By anchoring PCL-SH and PMEO2MA-SH onto the 14 nm AuNPs, a smart hybrid building block for self-assembly was obtained. Increasing the PCL/PMEO2MA chain ratio from 0.8:1, 2:1 and 3:1 to 7:1, the structure of gold assemblies (GAs) was observed to transfer from vesicle to large compound micelle (LCM). Contributed to the special dense packed structure of gold nanoparticles in LCM, the absorption spectrometry of gold nanoparticles drastically red-shifted from 520 nm to 830 nm, which endowed the GAs remarkable NIR photothermal conversion ability. In addition, gold has high X-ray absorption coefficient which qualifies gold nanomaterial a potential CT contrast agent Herein, we obtain a novel gold assembly structure which can be utilized as potential photothermal therapeutic and CT contrast agents. In vitro and In vivo studies testified the excellent treatment efficacy of optimum GAs as a PTT and CT contrast agent. In vitro degradation test, MTT assay and histology study indicated that GAs was a safe, low toxic reagent with good biodegradability. Therefore, the optimum GAs with strong NIR absorption and high X-ray absorption coefficient could be used as a theranostic agent and the formation of novel gold large compound micelle might offers a new theory foundation for engineering design and synthesis of polymer grafted AuNPs for biomedical applications.

Construction and Test Results of Coil 2 of a Three-Coil 800-MHz REBCO Insert for the 1.3-GHz High-Resolution NMR Magnet.

PubMed

Bascuñán, Juan; Michael, Philip; Hahn, Seungyong; Lecrevisse, Thibault; Iwasa, Yukikazu

2017-06-01

This paper focuses on the construction and test results of Coil 2 that is part of a trio of nested coils composing the REBCO 800 MHz insert. Upon its completion, the REBCO 800 MHz insert will be placed in the bore of a 500 MHz low temperature superconducting (LTS) NMR magnet (L500) to form the MIT 1.3 GHz high-resolution NMR magnet. Coil 2 is a stack of 32 double pancake (DP) coils wound with 6-mm wide REBCO tape using the no-insulation (NI) technique. Each pancake is wound on a stainless steel inner supporting ring to prevent the collapsing of its crossover due to the external pressure exerted by the winding pack. Coil 2 will be constructed in the following sequence: 1) after winding each DP will be individually tested in a bath of liquid nitrogen at atmospheric pressure to determine its current carrying capabilities; 2) DPs will be then assembled as a stack with interconnecting joints, and 3) as in Coil 1, each pancake will be overbanded with a stainless steel tape, this time to a thickness of 5 mm, thickness determined by a stress analysis previously performed. Finally the fully assembled Coil 2 will be tested in liquid nitrogen at 77 K and then in liquid helium at 4.2 K. We present here details of the stress analysis leading to the sizing of the DP inner supporting stainless steel ring and of the overbanding thickness required. Test results include coil index, critical current, charging time constant.

Selected Lessons Learned through the ISS Design, Development, Assembly, and Operations: Applicability to International Cooperation for Standardization

NASA Technical Reports Server (NTRS)

Hirsch, David B.

2009-01-01

This slide presentation reviews selected lessons that were learned during the design, development, assembly and operation of the International Space Station. The critical importance of standards and common interfaces is emphasized to create a common operation environment that can lead to flexibility and adaptability.

Solvent-Tuned Self-Assembled Nanostructures of Chiral l/d-Phenylalanine Derivatives of Protoporphyrin IX

PubMed Central

Bobe, Mr Sharad R; Al Kobaisi, Mohammad; Bhosale, Sheshanath V; Bhosale, Sidhanath V

2015-01-01

Protoporphyrin IX is a naturally occurring amphiphilic porphyrin with a rigid hydrophobic nonpolar core and two polar propionic acid substitutions on the porphyrin ring. This molecule can be modified on the hydrophilic group, which can lead to strengthened π–π-stacking and spontaneous self-assembly into novel nanostructures. Herein, we use l- phenylalanine and d-phenylalanine to modify protoporphyrin IX, and use the two derivatives for solvophobic-controlled self-assembly. Both derivatives possess two important features: 1) the aromatic core of the porphyrin for dispersive interactions and 2) a chiral amino acid to maximize the influence of chirality on selfassembly. These derivatives lead to the formation of a variety of nanostructure morphologies, such as spheres, nanofibers, lamellar structures, and thread-like and spherical shells. Solution-based self-assembly was determined by UV/Vis, fluorescence, and circular dichroism spectroscopy, and the formed nanostructures were characterized by scanning electron microscopy (SEM). Such engineered porphyrin derivatives could have potential applications in energy transport and storage, supramolecular chemistry, materials science, and medicine. PMID:26478848

Solvent-Tuned Self-Assembled Nanostructures of Chiral l/d-Phenylalanine Derivatives of Protoporphyrin IX.

PubMed

Bobe, Mr Sharad R; Al Kobaisi, Mohammad; Bhosale, Sheshanath V; Bhosale, Sidhanath V

2015-08-01

Protoporphyrin IX is a naturally occurring amphiphilic porphyrin with a rigid hydrophobic nonpolar core and two polar propionic acid substitutions on the porphyrin ring. This molecule can be modified on the hydrophilic group, which can lead to strengthened π-π-stacking and spontaneous self-assembly into novel nanostructures. Herein, we use l- phenylalanine and d-phenylalanine to modify protoporphyrin IX, and use the two derivatives for solvophobic-controlled self-assembly. Both derivatives possess two important features: 1) the aromatic core of the porphyrin for dispersive interactions and 2) a chiral amino acid to maximize the influence of chirality on selfassembly. These derivatives lead to the formation of a variety of nanostructure morphologies, such as spheres, nanofibers, lamellar structures, and thread-like and spherical shells. Solution-based self-assembly was determined by UV/Vis, fluorescence, and circular dichroism spectroscopy, and the formed nanostructures were characterized by scanning electron microscopy (SEM). Such engineered porphyrin derivatives could have potential applications in energy transport and storage, supramolecular chemistry, materials science, and medicine.

Payload spin assembly for the commercial Titan launch vehicle

NASA Technical Reports Server (NTRS)

Robinson, Wilf; Pech, Greg

1991-01-01

A contract was completed to design, build, and test a Payload Spin Assembly (PSA) for installation onto the Martin Marietta Titan 3 Commercial launch vehicle. This assembly provides launch support for satellite payloads up to 5783 kilograms (6.37 tons) and controls release, spin-up, and final separation of the satellite from the second stage. Once separated, the satellite's Perigee Kick Motor (PKM) boosts the satellite into its transfer orbit. The first successful flight occurred December 31, 1989. Requirements, design, test, and problems associated with this mechanical assembly are discussed.

VIIRS-J1 Polarization Narrative

NASA Technical Reports Server (NTRS)

Waluschka, Eugene; McCorkel, Joel; McIntire, Jeff; Moyer, David; McAndrew, Brendan; Brown, Steven W.; Lykke, Keith; Butler, James; Meister, Gerhard; Thome, Kurtis J.

2015-01-01

The VIS/NIR bands polarization sensitivity of Joint Polar Satellite Sensor 1 (JPSS1) Visible/Infrared Imaging Radiometer Suite (VIIRS) instrument was measured using a broadband source. While polarization sensitivity for bands M5-M7, I1, and I2 was less than 2.5%, the maximum polarization sensitivity for bands M1, M2, M3, and M4 was measured to be 6.4%, 4.4%, 3.1%, and 4.3%, respectively with a polarization characterization uncertainty of less than 0.3%. A detailed polarization model indicated that the large polarization sensitivity observed in the M1 to M4 bands was mainly due to the large polarization sensitivity introduced at the leading and trailing edges of the newly manufactured VISNIR bandpass focal plane filters installed in front of the VISNIR detectors. This was confirmed by polarization measurements of bands M1 and M4 bands using monochromatic light. Discussed are the activities leading up to and including the instruments two polarization tests, some discussion of the polarization model and the model results, the role of the focal plane filters, the polarization testing of the Aft-Optics-Assembly, the testing of the polarizers at Goddard and NIST and the use of NIST's T-SIRCUS for polarization testing and associated analyses and results.

LPT. Shield test facility assembly and test building (TAN646), south ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LPT. Shield test facility assembly and test building (TAN-646), south end of EBOR helium wing. Camera facing north. Monorail protrudes from upper-level door. Rust marks on concrete wall are from stack. Metal shed is post-1970 addition. INEEL negative no. HD-40-8-1 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

Analysis of space environment damage to solar cell assemblies from LDEF experiment A0171-GSFC test plate

NASA Technical Reports Server (NTRS)

Hill, David C.; Rose, M. Frank

1994-01-01

The results of the postflight analysis of the solar cell assemblies from the LDEF (Long Duration Exposure facility) experiment A0171 is provided in this NASA sponsored research project. The following data on this research are provided as follows: (1) solar cell description, including, substrate composition and thickness, crystal orientation, anti-reflective coating composition and thickness; (2) preflight characteristics of the solar cell assemblies with respect to current and voltage; and (3) post-flight characteristics of the solar cell assemblies with respect to voltage and current. These solar cell assemblies are part of the Goddard Space Flight Center test plate which was designed to test the space environment effects (radiation, atomic oxygen, thermal cycling, meteoroid and debris) on conductively coated solar cell coversheets, various electrical bond materials, solar cell performance, and other material properties where feasible.

An expert system executive for automated assembly of large space truss structures

NASA Technical Reports Server (NTRS)

Allen, Cheryl L.

1993-01-01

Langley Research Center developed a unique test bed for investigating the practical problems associated with the assembly of large space truss structures using robotic manipulators. The test bed is the result of an interdisciplinary effort that encompasses the full spectrum of assembly problems - from the design of mechanisms to the development of software. The automated structures assembly test bed and its operation are described, the expert system executive and its development are detailed, and the planned system evolution is discussed. Emphasis is on the expert system implementation of the program executive. The executive program must direct and reliably perform complex assembly tasks with the flexibility to recover from realistic system errors. The employment of an expert system permits information that pertains to the operation of the system to be encapsulated concisely within a knowledge base. This consolidation substantially reduced code, increased flexibility, eased software upgrades, and realized a savings in software maintenance costs.

Allosteric Control of Icosahedral Capsid Assembly

PubMed Central

Lazaro, Guillermo R.

2017-01-01

During the lifecycle of a virus, viral proteins and other components self-assemble to form an ordered protein shell called a capsid. This assembly process is subject to multiple competing constraints, including the need to form a thermostable shell while avoiding kinetic traps. It has been proposed that viral assembly satisfies these constraints through allosteric regulation, including the interconversion of capsid proteins among conformations with different propensities for assembly. In this article we use computational and theoretical modeling to explore how such allostery affects the assembly of icosahedral shells. We simulate assembly under a wide range of protein concentrations, protein binding affinities, and two different mechanisms of allosteric control. We find that, above a threshold strength of allosteric control, assembly becomes robust over a broad range of subunit binding affinities and concentrations, allowing the formation of highly thermostable capsids. Our results suggest that allostery can significantly shift the range of protein binding affinities that lead to successful assembly, and thus should be accounted for in models that are used to estimate interaction parameters from experimental data. PMID:27117092

Asymmetry of intronic pre-miRNA structures in functional RISC assembly

PubMed Central

Lin, Shi-Lung; Chang, Donald; Ying, Shao-Yao

2006-01-01

The two oligonucleotide strands of a siRNA duplex are functionally asymmetric in assembling the RNAi effector, RNA-induced gene silencing complex (RISC). Based on this asymmetric RISC assembly model in vitro, formation of a microRNA (miRNA) and complementary miRNA (miRNA*) duplex was proposed to be an essential step for the assembly of miRNA-associated RISC (miRISC). We observed here that a strong structural bias exists in the selection of a mature miRNA strand for RISC assembly in zebrafish using an intronic miRNA-like vector to target EGFP mRNA for regulation. The position of the stemloop in a precursor miRNA (pre-miRNA) was involved in the determination of miRNA–miRNA* asymmetry of the pre-miRNA stemarm, leading to different miRNA maturation during miRISC assembly. These findings suggest that the miRISC assembly is likely different from the RISC assembly model of siRNA in zebrafish, providing the first in vivo evidence for asymmetric miRISC assembly. PMID:16005165

75 FR 68688 - Airworthiness Directives; The Boeing Company Model 757 Airplanes

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-09

...We are adopting a new airworthiness directive (AD) for all Model 757 airplanes. This AD requires changing the lower fixed leading edge panel assemblies immediately outboard of the nacelles at slats 4 and 7. This AD results from reports of Model 757 airplanes in service that have drain holes and unsealed panel assemblies in the fixed leading edge adjacent to the inboard end of slats 4 and 7 that are too close to the hot portion of the engines. We are issuing this AD to prevent fuel leaking onto an engine and a consequent fire.

Self-assembly of self-limiting monodisperse supraparticles from polydisperse nanoparticles

NASA Astrophysics Data System (ADS)

Xia, Yunsheng; Nguyen, Trung Dac; Yang, Ming; Lee, Byeongdu; Santos, Aaron; Podsiadlo, Paul; Tang, Zhiyong; Glotzer, Sharon C.; Kotov, Nicholas A.

2011-09-01

Nanoparticles are known to self-assemble into larger structures through growth processes that typically occur continuously and depend on the uniformity of the individual nanoparticles. Here, we show that inorganic nanoparticles with non-uniform size distributions can spontaneously assemble into uniformly sized supraparticles with core-shell morphologies. This self-limiting growth process is governed by a balance between electrostatic repulsion and van der Waals attraction, which is aided by the broad polydispersity of the nanoparticles. The generic nature of the interactions creates flexibility in the composition, size and shape of the constituent nanoparticles, and leads to a large family of self-assembled structures, including hierarchically organized colloidal crystals.

Transition Behaviors of Configurations of Colloidal Particles at a Curved Oil-Water Interface

PubMed Central

Lee, Mina; Xia, Ming; Park, Bum Jun

2016-01-01

We studied the transition behaviors of colloidal arrangements confined at a centro-symmetrically curved oil-water interface. We found that assemblies composed of several colloidal particles at the curved interface exhibit at least two unique patterns that can be attributed to two factors: heterogeneity of single-colloid self-potential and assembly kinetics. The presence of the two assembly structures indicates that an essential energy barrier between the two structures exists and that one of the structures is kinetically stable. This energy barrier can be overcome via external stimuli (e.g., convection and an optical force), leading to dynamic transitions of the assembly patterns. PMID:28773263

HSV-1 Glycoproteins Are Delivered to Virus Assembly Sites Through Dynamin-Dependent Endocytosis.

PubMed

Albecka, Anna; Laine, Romain F; Janssen, Anne F J; Kaminski, Clemens F; Crump, Colin M

2016-01-01

Herpes simplex virus-1 (HSV-1) is a large enveloped DNA virus that belongs to the family of Herpesviridae. It has been recently shown that the cytoplasmic membranes that wrap the newly assembled capsids are endocytic compartments derived from the plasma membrane. Here, we show that dynamin-dependent endocytosis plays a major role in this process. Dominant-negative dynamin and clathrin adaptor AP180 significantly decrease virus production. Moreover, inhibitors targeting dynamin and clathrin lead to a decreased transport of glycoproteins to cytoplasmic capsids, confirming that glycoproteins are delivered to assembly sites via endocytosis. We also show that certain combinations of glycoproteins colocalize with each other and with the components of clathrin-dependent and -independent endocytosis pathways. Importantly, we demonstrate that the uptake of neutralizing antibodies that bind to glycoproteins when they become exposed on the cell surface during virus particle assembly leads to the production of non-infectious HSV-1. Our results demonstrate that transport of viral glycoproteins to the plasma membrane prior to endocytosis is the major route by which these proteins are localized to the cytoplasmic virus assembly compartments. This highlights the importance of endocytosis as a major protein-sorting event during HSV-1 envelopment. © 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

The effect of self-sorting and co-assembly on the mechanical properties of low molecular weight hydrogels

NASA Astrophysics Data System (ADS)

Colquhoun, Catherine; Draper, Emily R.; Eden, Edward G. B.; Cattoz, Beatrice N.; Morris, Kyle L.; Chen, Lin; McDonald, Tom O.; Terry, Ann E.; Griffiths, Peter C.; Serpell, Louise C.; Adams, Dave J.

2014-10-01

Self-sorting in low molecular weight hydrogels can be achieved using a pH triggered approach. We show here that this method can be used to prepare gels with different types of mechanical properties. Cooperative, disruptive or orthogonal assembled systems can be produced. Gels with interesting behaviour can be also prepared, for example self-sorted gels where delayed switch-on of gelation occurs. By careful choice of gelator, co-assembled structures can also be generated, which leads to synergistic strengthening of the mechanical properties.Self-sorting in low molecular weight hydrogels can be achieved using a pH triggered approach. We show here that this method can be used to prepare gels with different types of mechanical properties. Cooperative, disruptive or orthogonal assembled systems can be produced. Gels with interesting behaviour can be also prepared, for example self-sorted gels where delayed switch-on of gelation occurs. By careful choice of gelator, co-assembled structures can also be generated, which leads to synergistic strengthening of the mechanical properties. Electronic supplementary information (ESI) available: Full experimental and synthetic details for the dipeptides, full experimental descriptions, further NMR, single crystal diffraction data, fXRD data and SANS data. See DOI: 10.1039/c4nr04039b

Film Self-Assembly of Oppositely Charged Macromolecules Triggered by Electrochemistry through a Morphogenic Approach.

PubMed

Dochter, Alexandre; Garnier, Tony; Pardieu, Elodie; Chau, Nguyet Trang Thanh; Maerten, Clément; Senger, Bernard; Schaaf, Pierre; Jierry, Loïc; Boulmedais, Fouzia

2015-09-22

The development of new surface functionalization methods that are easy to use, versatile, and allow local deposition represents a real scientific challenge. Overcoming this challenge, we present here a one-pot process that consists in self-assembling, by electrochemistry on an electrode, films made of oppositely charged macromolecules. This method relies on a charge-shifting polyanion, dimethylmaleic-modified poly(allylamine) (PAHd), that undergoes hydrolysis at acidic pH, leading to an overall switching of its charge. When a mixture of the two polyanions, PAHd and poly(styrenesulfonate) (PSS), is placed in contact with an electrode, where the pH is decreased locally by electrochemistry, the transformation of PAHd into a polycation (PAH) leads to the continuous self-assembly of a nanometric PAH/PSS film by electrostatic interactions. The pH decrease is obtained by the electrochemical oxidation of hydroquinone, which produces protons locally over nanometric distances. Using a negatively charged enzyme, alkaline phosphatase (AP), instead of PSS, this one-pot process allows the creation of enzymatically active films. Under mild conditions, self-assembled PAH/AP films have an enzymatic activity which is adjustable simply by controlling the self-assembly time. The selective functionalization of microelectrode arrays by PAH/AP was achieved, opening the route toward miniaturized biosensors.

Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal closure

PubMed Central

Laplante, Caroline

2011-01-01

During Drosophila melanogaster dorsal closure, lateral sheets of embryonic epidermis assemble an actomyosin cable at their leading edge and migrate dorsally over the amnioserosa, converging at the dorsal midline. We show that disappearance of the homophilic cell adhesion molecule Echinoid (Ed) from the amnioserosa just before dorsal closure eliminates homophilic interactions with the adjacent dorsal-most epidermal (DME) cells, which comprise the leading edge. The resulting planar polarized distribution of Ed in the DME cells is essential for the localized accumulation of actin regulators and for actomyosin cable formation at the leading edge and for the polarized localization of the scaffolding protein Bazooka/PAR-3. DME cells with uniform Ed fail to assemble a cable and protrude dorsally, suggesting that the cable restricts dorsal migration. The planar polarized distribution of Ed in the DME cells thus provides a spatial cue that polarizes the DME cell actin cytoskeleton, defining the epidermal leading edge and establishing its contractile properties. PMID:21263031

78 FR 45104 - Model Manufactured Home Installation Standards: Ground Anchor Installations

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-26

... test methods for establishing working load design values of ground anchor assemblies used for new... anchor installations and establish standardized test methods to determine ground anchor performance and... currently no national test method for rating and certifying ground anchor assemblies in different soil...

1,2,4,5-Benzenetetracarboxylic acid: a versatile hydrogen bonding template for controlling the regioselective topochemical synthesis of head-to-tail photodimers from stilbazole derivatives.

PubMed

Ortega, Gabriela; Hernández, Jesús; González, Teresa; Dorta, Romano; Briceño, Alexander

2018-05-16

The crystal engineering of hydrogen bonded organic assemblies based on 1,2,4,5-benzenetetracarboxylic acid (H4bta) and stilbazole derivatives (1-10) is exploited to provide regio-controlled [2 + 2] photocycloadditions in the solid state. Single crystal X-ray diffraction analyses have revealed that all the arrays are built-up from the self-assembly of the (H2bta)2- dianion with two stilbazolium cations via O-HO- and N+-HO- charge-assisted H-bonding synthons: (4-Hstilbazolium+)2(H2bta2-). The dianion displays an interesting diversity of H-bonding motifs. Such structural flexibility allowed us to obtain four structure-types defined by the preferential formation of intramolecular or intermolecular hydrogen bonds between carboxylate-carboxylic groups. In these ionic assemblies two predominant structural H-bonding patterns were observed. The first pattern is characterised by the formation of intramolecular H-bonds in the dianion, leading to discrete assemblies based on ternary arrays. The second hydrogen pattern consists of 2-D hydrogen networks built-up from the self-assembly of anions via intermolecular H-bonds that are linked to the cations. Two additional examples, in which the dianion is self-assembled in two types of ribbons, were also observed. Another supramolecular feature predominant in all these arrays is the stacking of the cations in a head-to-tail fashion, which is controlled via cation-π interactions. These arrays are photoactive in the solid state upon UV-irradiation leading to the regioselective synthesis of rctt-cyclobutane head-to-tail-isomers in high to quantitative yield. In this work, the template tolerance either to steric or electronic effects by changing the number or positions of the supramolecular interactions exerted by distinctive functional groups was also explored. In addition, assemblies bearing 2-chloro (7 and 8) and 3-chloro-4-stilbazole (1 and 9) crystallize in two different crystalline forms, leading to novel examples of supramolecular isomers with similar solid state reactivity.

Opto-electronic devices with nanoparticles and their assemblies

NASA Astrophysics Data System (ADS)

Nguyen, Chieu Van

Nanotechnology is a fast growing field; engineering matters at the nano-meter scale. A key nanomaterial is nanoparticles (NPs). These sub-wavelength (< 100nm) particles provide tremendous possibilities due to their unique electrical, optical, and mechanical properties. Plethora of NPs with various chemical composition, size and shape has been synthesized. Clever designs of sub-wavelength structures enable observation of unusual properties of materials, and have led to new areas of research such as metamaterials. This dissertation describes two self-assemblies of gold nanoparticles, leading to an ultra-soft thin film and multi-functional single electron device at room temperature. First, the layer-by-layer self-assembly of 10nm Au nanoparticles and polyelectrolytes is shown to behave like a cellular-foam with modulus below 100 kPa. As a result, the composite thin film (˜ 100nm) is 5 orders of magnitude softer than an equally thin typical polymer film. The thin film can be compressed reversibly to 60% strain. The extraordinarily low modulus and high compressibility are advantageous in pressure sensing applications. The unique mechanical properties of the composite film lead to development of an ultra-sensitive tactile imaging device capable of screening for breast cancer. On par with human finger sensitivity, the tactile device can detect a 5mm imbedded object up to 20mm below the surface with low background noise. The second device is based on a one-dimensional (1-D) self-directed self-assembly of Au NPs mediated by dielectric materials. Depending on the coverage density of the Au NPs assembly deposited on the device, electronic emission was observed at ultra-low bias of 40V, leading to low-power plasma generation in air at atmospheric pressure. Light emitted from the plasma is apparent to the naked eyes. Similarly, 1-D self-assembly of Au NPs mediated by iron oxide was fabricated and exhibits ferro-magnetic behavior. The multi-functional 1-D self-assembly of Au NPs has great potential in modern electronics such as solid state lighting, plasma-based nanoelectronics, and memory devices.

Demonstrating the Effect of Particle Impact Dampers on the Random Vibration Response and Fatigue Life of Printed Wiring Assemblies

NASA Technical Reports Server (NTRS)

Knight, Brent; Montgomery, Randall; Geist, David; Hunt, Ron; LaVerde, Bruce; Towner, Robert

2013-01-01

In a recent experimental study, small Particle Impact Dampers (PID) were bonded directly to the surface of printed circuit board (PCB) or printed wiring assemblies (PWA), reducing the random vibration response and increasing the fatigue life. This study provides data verifying practicality of this approach. The measured peak strain and acceleration response of the fundamental out of plane bending mode was significantly attenuated by adding a PID device. Attenuation of this mode is most relevant to the fatigue life of a PWA because the local relative displacements between the board and the supported components, which ultimately cause fatigue failures of the electrical leads of the board-mounted components are dominated by this mode. Applying PID damping at the board-level of assembly provides mitigation with a very small mass impact, especially as compared to isolation at an avionics box or shelf level of assembly. When compared with other mitigation techniques at the PWA level (board thickness, stiffeners, constrained layer damping), a compact PID device has the additional advantage of not needing to be an integral part of the design. A PID can simply be bonded to heritage or commercial off the shelf (COTS) hardware to facilitate its use in environments beyond which it was originally qualified. Finite element analysis and test results show that the beneficial effect is not localized and that the attenuation is not due to the simple addition of mass. No significant, detrimental reduction in frequency was observed. Side-by-side life testing of damped and un-damped boards at two different thicknesses (0.070" and 0.090") has shown that the addition of a PID was much more significant to the fatigue life than increasing the thickness. High speed video, accelerometer, and strain measurements have been collected to correlate with analytical results.

Kilopower: Small and Affordable Fission Power Systems for Space

NASA Technical Reports Server (NTRS)

Mason, Lee; Palac, Don; Gibson, Marc

2017-01-01

The Nuclear Systems Kilopower Project was initiated by NASA's Space Technology Mission Directorate Game Changing Development Program in fiscal year 2015 to demonstrate subsystem-level technology readiness of small space fission power in a relevant environment (Technology Readiness Level 5) for space science and human exploration power needs. The Nuclear Systems Kilopower Project centerpiece is the Kilopower Reactor Using Stirling Technology (KRUSTY) test, which consists of the development and testing of a fission ground technology demonstrator of a 1 kWe-class fission power system. The technologies to be developed and validated by KRUSTY are extensible to space fission power systems from 1 to 10 kWe, which can enable higher power future potential deep space science missions, as well as modular surface fission power systems for exploration. The Kilopower Project is cofounded by NASA and the Department of Energy National Nuclear Security Administration (NNSA).KRUSTY include the reactor core, heat pipes to transfer the heat from the core to the power conversion system, and the power conversion system. Los Alamos National Laboratory leads the design of the reactor, and the Y-12 National Security Complex is fabricating it. NASA Glenn Research Center (GRC) has designed, built, and demonstrated the balance of plant heat transfer and power conversion portions of the KRUSTY experiment. NASA MSFC developed an electrical reactor simulator for non-nuclear testing, and the design of the reflector and shielding for nuclear testing. In 2016, an electrically heated non-fissionable Depleted Uranium (DU) core was tested at GRC in a configuration identical to the planned nuclear test. Once the reactor core has been fabricated and shipped to the Device Assembly Facility at the NNSAs Nevada National Security Site, the KRUSTY nuclear experiment will be assembled and tested. Completion of the KRUSTY experiment will validate the readiness of 1 to 10 kWe space fission technology for NASAs future requirements for sunlight-independent space power. An early opportunity for demonstration of In-Situ Resource Utilization (ISRU) capability on the surface of Mars is currently being considered for 2026 launch. Since a space fission system is the leading option for power generation for the first Mars human outpost, a smaller version of a planetary surface fission power system could be built to power the ISRU demonstration and ensure its end-to-end validity. Planning is underway to start the hardware development of this subscale flight demonstrator in 2018.

LPT. Low power assembly and test building (TAN640). Camera facing ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LPT. Low power assembly and test building (TAN-640). Camera facing west. Rollup doors to each test cell face east. Concrete walls poured in place. Apparatus at right of view was part of a post-ANP program. INEEL negative no. HD-40-1-1 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

Status of the eROSITA Telescope testing and calibrating the x-ray mirror assemblies

NASA Astrophysics Data System (ADS)

Burwitz, Vadim; Predehl, Peter; Bräuninger, Heinrich; Burkert, Wolfgang; Dennerl, Konrad; Eder, Josef; Friedrich, Peter; Fürmetz, Maria; Grisoni, Gabriele; Hartner, Gisela; Marioni, Fabio; Menz, Benedikt; Pfeffermann, Elmar; Valsecchi, Giuseppe

2013-09-01

The eROSITA X-ray observatory that will be launched on board the Russian Spectrum-RG mission comprises seven X-ray telescopes, each with its own mirror assembly (mirror module + X-ray baffle), electron deflector, filter wheel, and CCD camera with its control electronics. The completed flight mirror modules are undergoing many thorough X-ray tests at the PANTHER X-ray test facility after delivery, after being mated with the X-ray baffle, and again after both the vibration and thermal-vacuum tests. A description of the work done with mirror modules/assemblies and the test results obtained will be reported here. We report also on the environmental tests that have been performed on the eROSITA telescope qualification model.

Development Status of the Fission Power System Technology Demonstration Unit

NASA Technical Reports Server (NTRS)

Briggs, Maxwell H.; Gibson, Marc A.; Geng, Steven M.; Pearson, Jon Boise; Godfoy, Thomas

2012-01-01

This paper summarizes the progress that has been made in the development of the Fission Power System Technology Demonstration Unit (TDU). The reactor simulator core and Annular Linear Induction Pump have been fabricated and assembled into a test loop at the NASA Marshall Space Flight Center. A 12 kWe Power Conversion Unit (PCU) is being developed consisting of two 6 kWe free-piston Stirling engines. The two 6 kWe engines have been fabricated by Sunpower Inc. and are currently being tested separately prior to integration into the PCU. The Facility Cooling System (FCS) used to reject convertor waste heat has been assembled and tested at the NASA Glenn Research Center (GRC). The structural elements, including a Buildup Assembly Platform (BAP) and Upper Truss Structure (UTS) have been fabricated, and will be used to test cold-end components in thermal vacuum prior to TDU testing. Once all components have been fully tested at the subsystem level, they will be assembled into an end-to-end system and tested in thermal vacuum at GRC.

Base excitation testing system using spring elements to pivotally mount wind turbine blades

DOEpatents

Cotrell, Jason; Hughes, Scott; Butterfield, Sandy; Lambert, Scott

2013-12-10

A system (1100) for fatigue testing wind turbine blades (1102) through forced or resonant excitation of the base (1104) of a blade (1102). The system (1100) includes a test stand (1112) and a restoring spring assembly (1120) mounted on the test stand (1112). The restoring spring assembly (1120) includes a primary spring element (1124) that extends outward from the test stand (1112) to a blade mounting plate (1130) configured to receive a base (1104) of blade (1102). During fatigue testing, a supported base (1104) of a blad (1102) may be pivotally mounted to the test stand (1112) via the restoring spring assembly (1120). The system (1100) may include an excitation input assembly (1140) that is interconnected with the blade mouting plate (1130) to selectively apply flapwise, edgewise, and/or pitch excitation forces. The restoring spring assemply (1120) may include at least one tuning spring member (1127) positioned adjacent to the primary spring element (1124) used to tune the spring constant or stiffness of the primary spring element (1124) in one of the excitation directions.

Development Status of the Fission Power System Technology Demonstration Unit

NASA Technical Reports Server (NTRS)

Briggs, Maxwell H.; Gibson, Marc A.; Geng, Steven M; Pearson, Jon Boise; Godfroy, Thomas

2012-01-01

This paper summarizes the progress that has been made in the development of the Fission Power System Technology Demonstration Unit (TDU). The reactor simulator core and Annular Linear Induction Pump have been fabricated and assembled into a test loop at the NASA Marshall Space Flight Center. A 12 kWe Power Conversion Unit (PCU) is being developed consisting of two 6 kWe free-piston Stirling engines. The two 6 kWe engines have been fabricated by Sunpower Inc. and are currently being tested separately prior to integration into the PCU. The Facility Cooling System (FCS) used to reject convertor waste heat has been assembled and tested at the NASA Glenn Research Center (GRC). The structural elements, including a Buildup Assembly Platform (BAP) and Upper Truss Structure (UTS) have been fabricated, and will be used to test cold-end components in thermal vacuum prior to TDU testing. Once all components have been fully tested at the subsystem level, they will be assembled into an end-to-end system and tested in thermal vacuum at NASA GRC.

Cryogenic Optical Performance of a Lightweighted Mirror Assembly for Future Space Astronomical Telescopes: Correlating Optical Test Results and Thermal Optical Model

NASA Technical Reports Server (NTRS)

Eng, Ron; Arnold, William R.; Baker, Marcus A.; Bevan, Ryan M.; Burdick, Gregory; Effinger, Michael R.; Gaddy, Darrell E.; Goode, Brian K.; Hanson, Craig; Hogue, William D.;

Assessment of lead health hazards in a body shop of an automobile assembly plant.

PubMed

Lilis, R; Valciukas, J A; Kon, S; Sarkosi, L; Campbell, C; Selikoff, I J

1982-01-01

This study of an occupationally lead-exposed group in the automobile assembly industry (body shop employees) has provided information with regard to current and recent lead-exposure levels characteristic for these operations. Comparison with findings in other, previously studied occupational groups (secondary smelter workers) indicated that current and recent lead exposure was significantly lower in the autobody shop employees. Prevalence of symptoms was found to be higher than in a control, non-lead-exposed group of maintenance workers in the same plant, but the differences reached the level of statistical significance only sporadically. Long-term effects on renal function, as reflected in blood urea nitrogen (BUN) and creatinine levels, were documented to be significant in this group of autobody shop employees. When compared with two secondary lead smelter populations previously studied, correlations between indicators of renal function, BUN and creatinine, and duration of lead exposure were consistently higher in the secondary lead smelter populations than in the group of autobody shop employees, although duration of lead exposure was greater in the latter. These findings are consistent with a dose-response relationship with regard to renal function impairment due to lead absorption. An increased prevalence of hypertension, especially diastolic, was also found, probably the consequence of renal effect.

airborne data analysis/monitor system

NASA Technical Reports Server (NTRS)

Stephison, D. B.

1981-01-01

An Airborne Data Analysis/Monitor System (ADAMS), a ROLM 1666 computer based system installed onboard test airplanes used during experimental testing is evaluated. In addition to the 1666 computer, the ADAMS hardware includes a DDC System 90 fixed head disk and a Miltape DD400 floppy disk. Boeing designed a DMA interface to the data acquisition system and an intelligent terminal to reduce system overhead and simplify operator commands. The ADAMS software includes RMX/RTOS and both ROLM FORTRAN and assembly language are used. The ADAMS provides real time displays that enable onboard test engineers to make rapid decisions about test conduct thus reducing the cost and time required to certify new model airplanes, and improved the quality of data derived from the test, leading to more rapid development of improvements resulting in quieter, safer, and more efficient airplanes. The availability of airborne data processing removes most of the weather and geographical restrictions imposed by telemetered flight test data systems. A data base is maintained to describe the airplane, the data acquisition system, the type of testing, and the conditions under which the test is performed.

KSC-2009-3672

NASA Image and Video Library

2009-06-11

CAPE CANAVERAL, Fla. – At NASA's Kennedy Space Center in Florida, employees gather to watch the Ares I-X forward assembly (comprising the frustum, forward skirt extension and forward skirt) as it moves out of the Assembly and Refurbishment Facility. The assembly is being transferred to the Vehicle Assembly Building's High Bay 4 for processing and stacking to the upper stage. Ares I-X is the flight test for the Ares I which will provide NASA an early opportunity to test and prove hardware, facilities and ground operations associated with Ares I, which is part of the Constellation Program to return men to the moon and beyond. Launch of the Ares I-X flight test is targeted for August 2009. Photo credit: NASA/Jack Pfaller

Assembly processes comparison for a miniaturized laser used for the Exomars European Space Agency mission

NASA Astrophysics Data System (ADS)

Ribes-Pleguezuelo, Pol; Inza, Andoni Moral; Basset, Marta Gilaberte; Rodríguez, Pablo; Rodríguez, Gemma; Laudisio, Marco; Galan, Miguel; Hornaff, Marcel; Beckert, Erik; Eberhardt, Ramona; Tünnermann, Andreas

2016-11-01

A miniaturized diode-pumped solid-state laser (DPSSL) designed as part of the Raman laser spectrometer (RLS) instrument for the European Space Agency (ESA) Exomars mission 2020 is assembled and tested for the mission purpose and requirements. Two different processes were tried for the laser assembling: one based on adhesives, following traditional laser manufacturing processes; another based on a low-stress and organic-free soldering technique called solderjet bumping technology. The manufactured devices were tested for the processes validation by passing mechanical, thermal cycles, radiation, and optical functional tests. The comparison analysis showed a device improvement in terms of reliability of the optical performances from the soldered to the assembled by adhesive-based means.

Highly specific salt bridges govern bacteriophage P22 icosahedral capsid assembly: identification of the site in coat protein responsible for interaction with scaffolding protein.

PubMed

Cortines, Juliana R; Motwani, Tina; Vyas, Aashay A; Teschke, Carolyn M

2014-05-01

Icosahedral virus assembly requires a series of concerted and highly specific protein-protein interactions to produce a proper capsid. In bacteriophage P22, only coat protein (gp5) and scaffolding protein (gp8) are needed to assemble a procapsid-like particle, both in vivo and in vitro. In scaffolding protein's coat binding domain, residue R293 is required for procapsid assembly, while residue K296 is important but not essential. Here, we investigate the interaction of scaffolding protein with acidic residues in the N-arm of coat protein, since this interaction has been shown to be electrostatic. Through site-directed mutagenesis of genes 5 and 8, we show that changing coat protein N-arm residue 14 from aspartic acid to alanine causes a lethal phenotype. Coat protein residue D14 is shown by cross-linking to interact with scaffolding protein residue R293 and, thus, is intimately involved in proper procapsid assembly. To a lesser extent, coat protein N-arm residue E18 is also implicated in the interaction with scaffolding protein and is involved in capsid size determination, since a cysteine mutation at this site generated petite capsids. The final acidic residue in the N-arm that was tested, E15, is shown to only weakly interact with scaffolding protein's coat binding domain. This work supports growing evidence that surface charge density may be the driving force of virus capsid protein interactions. Bacteriophage P22 infects Salmonella enterica serovar Typhimurium and is a model for icosahedral viral capsid assembly. In this system, coat protein interacts with an internal scaffolding protein, triggering the assembly of an intermediate called a procapsid. Previously, we determined that there is a single amino acid in scaffolding protein required for P22 procapsid assembly, although others modulate affinity. Here, we identify partners in coat protein. We show experimentally that relatively weak interactions between coat and scaffolding proteins are capable of driving correctly shaped and sized procapsids and that the lack of these proper protein-protein interfaces leads to aberrant structures. The present work represents an important contribution supporting the hypothesis that virus capsid assembly is governed by seemingly simple interactions. The highly specific nature of the subunit interfaces suggests that these could be good targets for antivirals.

Debris protection cover assembly for cable connectors

NASA Technical Reports Server (NTRS)

Yovan, Roger D. (Inventor)

1999-01-01

A protective cover assembly for an end of a cable connector having a cable housing that encloses a plurality of connective pins or sockets and that satisfies all requirements for space applications. A connector body flange is formed at the extremity of a cable and is positioned so that it may register with a corresponding connector body flange on the end of a companion cable to which a connection is to be made, one cable end having cable lead pins and the companion cable end having lead sockets with which the pins register. A latch mechanism having a latch housing is received in the connector body flange and a crank connected to a manually rotatable cap actuates a spring-loaded latch element that is engageable with a connector body flange to secure or to release the cover assembly with the simple twisting motion of the cap, thereby simplifying the task of effecting coupling and decoupling of the cable ends.

Tailoring the vapor-liquid-solid growth toward the self-assembly of GaAs nanowire junctions.

PubMed

Dai, Xing; Dayeh, Shadi A; Veeramuthu, Vaithianathan; Larrue, Alexandre; Wang, Jian; Su, Haibin; Soci, Cesare

2011-11-09

New insights into understanding and controlling the intriguing phenomena of spontaneous merging (kissing) and the self-assembly of monolithic Y- and T-junctions is demonstrated in the metal-organic chemical vapor deposition growth of GaAs nanowires. High-resolution transmission electron microscopy for determining polar facets was coupled to electrostatic-mechanical modeling and position-controlled synthesis to identify nanowire diameter, length, and pitch, leading to junction formation. When nanowire patterns are designed so that the electrostatic energy resulting from the interaction of polar surfaces exceeds the mechanical energy required to bend the nanowires to the point of contact, their fusion can lead to the self-assembly of monolithic junctions. Understanding and controlling this phenomenon is a great asset for the realization of dense arrays of vertical nanowire devices and opens up new ways toward the large scale integration of nanowire quantum junctions or nanowire intracellular probes.

Infrared spectroscopy of organic semiconductors modified by self-assembled monolayers

NASA Astrophysics Data System (ADS)

Khatib, O.; Lee, B.; Podzorov, V.; Yuen, J.; Heeger, A. J.; Li, Z. Q.; di Ventra, M.; Basov, D. N.

2009-03-01

Recently, self-assembled monolayers (SAMs) were used to modify electronic surface properties of organic single crystals, leading to several orders of magnitude increase in the electrical conductivity^1. Motivated by this discovery, the same technique was applied to polymers. Here we present a thorough spectroscopic investigation of organic semiconductors based on poly(3-hexlthiophene) (P3HT) that have been treated with a fluorinated trichlorosilane SAM. Infrared spectroscopy offers access to details of charge injection, electrostatic doping, and the electronic structure that are not always available from transport measurements, which can be dominated by defects and contact effects. In polymer films, the SAM molecules penetrate into the bulk, leading to a rich spectrum of electronic excitations in the mid-infrared energy range. ^1 M. F. Calhoun, J. Sanchez, D. Olaya, M. E. Gershenson, V. Podzorov, Electronic functionalization of the surface of organic semiconductors with self-assembled monolayers, Nature Mater. 7, 84--89 (2008)

4. PROPOSED C1 ASSEMBLY AND TESTING FACILITIES FOR THE ORDINANCE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

4. PROPOSED C-1 ASSEMBLY AND TESTING FACILITIES FOR THE ORDINANCE GUIDED MISSILE CENTER AT REDSTONE ARSENAL, NEAR THE SOUTH END OF MADKIN MOUNTAIN. OCTOBER 1950, HANNES LUEHRSEN COLLECTION, MSFC MASTER PLANNING OFFICE. - Marshall Space Flight Center, East Test Area, Dodd Road, Huntsville, Madison County, AL

Filamin A Is a Regulator of Blood-Testis Barrier Assembly during Postnatal Development in the Rat Testis

PubMed Central

Su, Wenhui; Mruk, Dolores D.; Lie, Pearl P. Y.; Lui, Wing-yee

2012-01-01

The blood-testis barrier (BTB) is an important ultrastructure in the testis. A delay in its assembly during postnatal development leads to meiotic arrest. Also, a disruption of the BTB by toxicants in adult rats leads to a failure in spermatogonial differentiation. However, the regulation of BTB assembly remains unknown. Herein, filamin A, an actin filament cross-linker that is known to maintain and regulate cytoskeleton structure and function in other epithelia, was shown to be highly expressed during the assembly of Sertoli cell BTB in vitro and postnatal development of BTB in vivo, perhaps being used to maintain the actin filament network at the BTB. A knockdown of filamin A by RNA interference was found to partially perturb the Sertoli cell tight junction (TJ) permeability barrier both in vitro and in vivo. Interestingly, this down-regulating effect on the TJ barrier function after the knockdown of filamin A was associated with a mis-localization of both TJ and basal ectoplasmic specialization proteins. Filamin A knockdown also induced a disorganization of the actin filament network in Sertoli cells in vitro and in vivo. Collectively, these findings illustrate that filamin A regulates BTB assembly by recruiting these proteins to the microenvironment in the seminiferous epithelium to serve as the building blocks. In short, filamin A participates in BTB assembly by regulating protein recruitment during postnatal development in the rat testis. PMID:22872576

Disulfide-induced self-assembled targets: A novel strategy for the label free colorimetric detection of DNAs/RNAs via unmodified gold nanoparticles

NASA Astrophysics Data System (ADS)

Shokri, Ehsan; Hosseini, Morteza; Davari, Mehdi D.; Ganjali, Mohammad R.; Peppelenbosch, Maikel P.; Rezaee, Farhad

2017-04-01

A modified non-cross-linking gold-nanoparticles (Au-NPs) aggregation strategy has been developed for the label free colorimetric detection of DNAs/RNAs based on self-assembling target species in the presence of thiolated probes. Two complementary thiol- modified probes, each of which specifically binds at one half of the target introduced SH groups at both ends of dsDNA. Continuous disulfide bond formation at 3‧ and 5‧ terminals of targets leads to the self-assembly of dsDNAs into the sulfur- rich and flexible products with different lengths. These products have a high affinity for the surface of Au-NPs and efficiently protect the surface from salt induced aggregation. To evaluate the assay efficacy, a small part of the citrus tristeza virus (CTV) genome was targeted, leading to a detection limit of about 5 × 10-9 mol.L-1 over a linear ranged from 20 × 10-9 to 10 × 10-7 mol.L-1. This approach also exhibits good reproducibility and recovery levels in the presence of plant total RNA or human plasma total circulating RNA extracts. Self-assembled targets can be then sensitively distinguished from non-assembled or mismatched targets after gel electrophoresis. The disulfide reaction method and integrating self-assembled DNAs/RNAs targets with bare AuNPs as a sensitive indicator provide us a powerful and simple visual detection tool for a wide range of applications.

Integrated Advanced Microwave Sounding Unit-A (AMSU-A): Engineering Test Report: METSAT A2 Signal Processor (P/N 1331120-2, S/N F03) S/N 107

NASA Technical Reports Server (NTRS)

1998-01-01

This report presents a description of the tests performed, and the test data, for the A2 METSAT Signal Processor Assembly PN: 1331120-2, S/N F03. The assembly was tested in accordance with AE-26754, "METSAT Signal Processor Scan Drive Test and Integration Procedure."

Integrated Advanced Microwave Sounding Unit-A (AMSU-A): Engineering Test Report, METSAT A2 Signal Processor (P/N 1331120-2, S/N F04) S/N 108

NASA Technical Reports Server (NTRS)

1998-01-01

This report presents a description of the tests performed, and the test data, for the A2 METSAT Signal Processor Assembly PN: 1331120-2, S/N F04. The assembly was tested in accordance with AE-26754, "METSAT Signal Processor Scan Drive Test and Integration Procedure."

A space crane concept for performing on-orbit assembly

NASA Technical Reports Server (NTRS)

Dorsey, John T.

1992-01-01

The topics are presented in viewgraph form and include: in-space assembly and construction enhances future mission planning flexibility; in-space assembly and construction facility concept; space crane concept with mobile base; fundamental characteristics; space crane research approach; spacecraft component positioning and assembly test-bed; and articulating joint testbed.

49 CFR 572.184 - Shoulder assembly.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Shoulder assembly. 572.184 Section 572.184... Test Dummy, 50th Percentile Adult Male § 572.184 Shoulder assembly. (a) The shoulder (175-3000) is part... (b) of this section, the shoulder assembly shall meet performance requirements of paragraph (c) of...

49 CFR 572.184 - Shoulder assembly.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Shoulder assembly. 572.184 Section 572.184... Test Dummy, 50th Percentile Adult Male § 572.184 Shoulder assembly. (a) The shoulder (175-3000) is part... (b) of this section, the shoulder assembly shall meet performance requirements of paragraph (c) of...

KENNEDY SPACE CENTER, FLA. - Crawler-transporter (CT) number 2, moves away from the Vehicle Assembly Building with a Mobile Launcher Platform (MLP) on top on a test run to the launch pad. The CT recently underwent modifications to the cab. The CT moves Space Shuttle vehicles, situated on the MLP, between the VAB and launch pad. Moving on four double-tracked crawlers, the CT uses a laser guidance system and a leveling system for the journey that keeps the top of a Space Shuttle vertical within plus- or minus-10 minutes of arc. The system enables the CT-MLP-Shuttle to negotiate the ramp leading to the launch pads and keep the load level. Unloaded, the CT weighs 6 million pounds. Seen on top of the MLP are two tail service masts that support the fluid, gas and electrical requirements of the orbiter’s liquid oxygen and liquid hydrogen aft umbilicals.

NASA Image and Video Library

2003-08-18

KENNEDY SPACE CENTER, FLA. - Crawler-transporter (CT) number 2, moves away from the Vehicle Assembly Building with a Mobile Launcher Platform (MLP) on top on a test run to the launch pad. The CT recently underwent modifications to the cab. The CT moves Space Shuttle vehicles, situated on the MLP, between the VAB and launch pad. Moving on four double-tracked crawlers, the CT uses a laser guidance system and a leveling system for the journey that keeps the top of a Space Shuttle vertical within plus- or minus-10 minutes of arc. The system enables the CT-MLP-Shuttle to negotiate the ramp leading to the launch pads and keep the load level. Unloaded, the CT weighs 6 million pounds. Seen on top of the MLP are two tail service masts that support the fluid, gas and electrical requirements of the orbiter’s liquid oxygen and liquid hydrogen aft umbilicals.

Proven high-reliability assembly methods applied to avionics fiber-optics high-speed transceivers

NASA Astrophysics Data System (ADS)

Lauzon, Jocelyn; Leduc, Lorrain; Bessette, Daniel; Bélanger, Nicolas; Larose, Robert; Dion, Bruno

2012-06-01

Harsh environment avionics applications require operating temperature ranges that can extend to, and exceed -50 to 115°C. For obvious maintenance, management and cost arguments, product lifetimes as long as 20 years are also sought. This leads to mandatory long-term hermeticity that cannot be obtained with epoxy or silicone sealing; but only with glass seal or metal solder or brazing. A hermetic design can indirectly result in the required RF shielding of the component. For fiber-optics products, these specifications need to be compatible with the smallest possible size, weight and power consumption. The products also need to offer the best possible high-speed performances added to the known EMI immunity in the transmission lines. Fiber-optics transceivers with data rates per fiber channel up to 10Gbps are now starting to be offered on the market for avionics applications. Some of them are being developed by companies involved in the "normal environment" telecommunications market that are trying to ruggedize their products packaging in order to diversify their customer base. Another approach, for which we will present detailed results, is to go back to the drawing boards and design a new product that is adapted to proven MIL-PRF-38534 high-reliability packaging assembly methods. These methods will lead to the introduction of additional requirements at the components level; such as long-term high-temperature resistance for the fiber-optic cables. We will compare both approaches and demonstrate the latter, associated with the redesign, is the preferable one. The performance of the fiber-optic transceiver we have developed, in terms of qualification tests such as temperature cycling, constant acceleration, hermeticity, residual gaz analysis, operation under random vibration and mechanical shocks and accelerated lifetime tests will be presented. The tests are still under way, but so far, we have observed no performance degradation of such a product after more than 1050 hours of operation at 95°C.

Dual-axis resonance testing of wind turbine blades

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hughes, Scott; Musial, Walter; White, Darris

An apparatus (100) for fatigue testing test articles (104) including wind turbine blades. The apparatus (100) includes a test stand (110) that rigidly supports an end (106) of the test article (104). An actuator assembly (120) is attached to the test article (104) and is adapted for substantially concurrently imparting first and second forcing functions in first and second directions on the test article (104), with the first and second directions being perpendicular to a longitudinal axis. A controller (130) transmits first and second sets of displacement signals (160, 164) to the actuator assembly (120) at two resonant frequencies ofmore » the test system (104). The displacement signals (160, 164) initiate the actuator assembly (120) to impart the forcing loads to concurrently oscillate the test article (104) in the first and second directions. With turbine blades, the blades (104) are resonant tested concurrently for fatigue in the flapwise and edgewise directions.« less

Apparatus for testing high pressure injector elements

NASA Technical Reports Server (NTRS)

Myers, William Neill (Inventor); Scott, Ewell M. (Inventor); Forbes, John C. (Inventor); Shadoan, Michael D. (Inventor)

1995-01-01

An apparatus for testing and evaluating the spray pattern of high pressure fuel injector elements for use in supplying fuel to combustion engines is presented. Prior art fuel injector elements were normally tested by use of low pressure apparatuses which did not provide a purge to prevent mist from obscuring the injector element or to prevent frosting of the view windows; could utilize only one fluid during each test; and had their viewing ports positioned one hundred eighty (180 deg) apart, thus preventing optimum use of laser diagnostics. The high pressure fluid injector test apparatus includes an upper hub, an upper weldment or housing, a first clamp and stud/nut assembly for securing the upper hub to the upper weldment, a standoff assembly within the upper weldment, a pair of window housings having view glasses within the upper weldment, an injector block assembly and purge plate within the upper weldment for holding an injector element to be tested and evaluated, a lower weldment or housing, a second clamp and stud/nut assembly for securing the lower weldment to the upper hub, a third clamp and stud/nut assembly for securing the lower hub to the lower weldment, mechanisms for introducing fluid under high pressure for testing an injector element, and mechanisms for purging the apparatus to prevent frosting of view glasses within the window housings and to permit unobstructed viewing of the injector element.

Apparatus for testing high pressure injector elements

NASA Technical Reports Server (NTRS)

Myers, William Neill (Inventor); Scott, Ewell M. (Inventor); Forbes, John C. (Inventor); Shadoan, Michael D. (Inventor)

1993-01-01

An apparatus for testing and evaluating the spray pattern of high pressure fuel injector elements for use in supplying fuel to combustion engines is presented. Prior art fuel injector elements were normally tested by use of low pressure apparatuses which did not provide a purge to prevent mist from obscuring the injector element or to prevent frosting of the view windows; could utilize only one fluid during each test; and had their viewing ports positioned one hundred eighty (180 deg) apart, thus preventing optimum use of laser diagnostics. The high pressure fluid injector test apparatus includes an upper hub, an upper weldment or housing, a first clamp and stud/nut assembly for securing the upper hub to the upper weldment, a standoff assembly within the upper weldment, a pair of window housings having view glasses within the upper weldment, an injector block assembly and purge plate within the upper weldment for holding an injector element to be tested and evaluated, a lower weldment or housing, a second clamp and stud/nut assembly for securing the lower weldment to the upper weldment, a lower hub, a third clamp and stud/nut assembly for securing the lower hub to the lower weldment, mechanisms for introducing fluid under high pressure for testing an injector element, and mechanisms for purging the apparatus to prevent frosting of view glasses within the window housings and to permit unobstructed viewing of the injector element.

2. MISSILE ASSEMBLY BUILDING, RIGHT SIDE, LOOKING NORTH. NIKE ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

2. MISSILE ASSEMBLY BUILDING, RIGHT SIDE, LOOKING NORTH. - NIKE Missile Base C-84, Missile Test & Assembly Building, North of Launch Area Entrance Drive & east of Mess Hall, Barrington, Cook County, IL

Manufacture and Quality Control of Insert Coil with Real ITER TF Conductor

DOE PAGES

Ozeki, H.; Isono, T.; Uno, Y.; ...

2016-03-02

JAEA successfully completed the manufacture of the toroidal field (TF) insert coil (TFIC) for a performance test of the ITER TF conductor in the final design in cooperation with Hitachi, Ltd. The TFIC is a single-layer 8.875-turn solenoid coil with 1.44-m diameter. This will be tested for 68-kA current application in a 13-T external magnetic field. TFIC was manufactured in the following order: winding of the TF conductor, lead bending, fabrication of the electrical termination, heat treatment, turn insulation, installation of the coil into the support mandrel structure, vacuum pressure impregnation (VPI), structure assembly, and instrumentation. Here in this presentation,more » manufacture process and quality control status for the TFIC manufacturing are reported.« less

Field Trial on a Rack-mounted DC Power Supply System with 80-Ah Lithium-ion Batteries

NASA Astrophysics Data System (ADS)

Matsushima, Toshio

Using an industrial lithium-ion battery that has higher energy density than conventional valve-regulated lead-acid batteries, a rack-mounted DC-power-supply system was assembled and tested at a base transceiver station (BTS) offering actual services. A nominal output voltage and maximum output current of the system is 53.5V and 20A, respectively. An 80-Ah lithium-ion battery composed of 13 cells connected in series was applied in the system and maintained in a floating charge method. The DC-power-supply system was installed in a 19-inch power rack in the telecommunications equipment box at BTS. The characteristics of the 80Ah lithium-ion battery, specifications of the DC-power-supply system and field-test results were shown in this paper.

Experimental and analytical analysis of polarization and water transport behaviors of hydrogen alkaline membrane fuel cell

NASA Astrophysics Data System (ADS)

Huo, Sen; Zhou, Jiaxun; Wang, Tianyou; Chen, Rui; Jiao, Kui

2018-04-01

Experimental test and analytical modeling are conducted to investigate the operating behavior of an alkaline electrolyte membrane (AEM) fuel cell fed by H2/air (or O2) and explore the effect of various operating pressures on the water transfer mechanism. According to the experimental test, the cell performance is greatly improved through increasing the operating pressure gradient from anode to cathode which leads to significant liquid water permeation through the membrane. The high frequency resistance of the A901 alkaline membrane is observed to be relatively stable as the operating pressure varies based on the electrochemical impedance spectroscopy (EIS) method. Correspondingly, based on the modeling prediction, the averaged water content in the membrane electrode assembly (MEA) does not change too much which leads to the weak variation of membrane ohmic resistance. This reveals that the performance enhancement should give the credit to better electro-chemical reaction kinetics for both the anode and cathode, also prone by the EIS results. The reversion of water back diffusion direction across the membrane is also observed through analytical solution.

Hybrid Lead Halide Layered Perovskites with Silsesquioxane Interlayers.

PubMed

Kataoka, Sho; Kaburagi, Wako; Mochizuki, Hiroyuki; Kamimura, Yoshihiro; Sato, Kazuhiko; Endo, Akira

2018-01-01

Hybrid organic-lead halide perovskites exhibit remarkable properties as semiconductors and light absorbers. Here, we report the formation of silsesquioxane-lead halide hybrid layered perovskites. We prepared silsesquioxane with a cubic cage-like structure and fabricated hybrid silsesquioxane-lead halide layered perovskites in a self-assembled manner. It is demonstrated that the silsesquioxane maintain their cage-like structure between lead halide perovskite layers. The silsesquioxane-lead halide perovskites also show excitonic absorption and emission in the visible light region similar to typical lead halide layered perovskites.

77 FR 62260 - Niles America Wintech, Inc., Warehousing Division, a Valeo Company, Including On-Site Leased...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-12

..., Adecco Employment Services, Winchester, KY; Niles America Wintech, Inc., Assembly and Testing Division, a... former workers of Niles America Wintech, Inc., Warehousing Division and Assembly and Testing Division...

Inverse Problem in Self-assembly

NASA Astrophysics Data System (ADS)

Tkachenko, Alexei

2012-02-01

By decorating colloids and nanoparticles with DNA, one can introduce highly selective key-lock interactions between them. This leads to a new class of systems and problems in soft condensed matter physics. In particular, this opens a possibility to solve inverse problem in self-assembly: how to build an arbitrary desired structure with the bottom-up approach? I will present a theoretical and computational analysis of the hierarchical strategy in attacking this problem. It involves self-assembly of particular building blocks (``octopus particles''), that in turn would assemble into the target structure. On a conceptual level, our approach combines elements of three different brands of programmable self assembly: DNA nanotechnology, nanoparticle-DNA assemblies and patchy colloids. I will discuss the general design principles, theoretical and practical limitations of this approach, and illustrate them with our simulation results. Our crucial result is that not only it is possible to design a system that has a given nanostructure as a ground state, but one can also program and optimize the kinetic pathway for its self-assembly.

Imaging enzyme-triggered self-assembly of small molecules inside live cells

PubMed Central

Gao, Yuan; Shi, Junfeng; Yuan, Dan; Xu, Bing

2012-01-01

Self-assembly of small molecules in water to form nanofibers, besides generating sophisticated biomaterials, promises a simple system inside cells for regulating cellular processes. But lack of a convenient approach for studying the self-assembly of small molecules inside cells hinders the development of such systems. Here we report a method to image enzyme-triggered self-assembly of small molecules inside live cells. After linking a fluorophore to a self-assembly motif to make a precursor, we confirmed by 31P NMR and rheology that enzyme-triggered conversion of the precursor to a hydrogelator results in the formation of a hydrogel via self-assembly. The imaging contrast conferred by the nanofibers of the hydrogelators allowed the evaluation of intracellular self-assembly; the dynamics, and the localization of the nanofibers of the hydrogelators in live cells. This approach explores supramolecular chemistry inside cells and may lead to new insights, processes, or materials at the interface of chemistry and biology. PMID:22929790

Monodisperse self-assembly in a model with protein-like interactions

NASA Astrophysics Data System (ADS)

Wilber, Alex W.; Doye, Jonathan P. K.; Louis, Ard A.; Lewis, Anna C. F.

2009-11-01

We study the self-assembly behavior of patchy particles with "proteinlike" interactions that can be considered as a minimal model for the assembly of viral capsids and other shell-like protein complexes. We thoroughly explore the thermodynamics and dynamics of self-assembly as a function of the parameters of the model and find robust assembly of all target structures considered. Optimal assembly occurs in the region of parameter space where a free energy barrier regulates the rate of nucleation, thus preventing the premature exhaustion of the supply of monomers that can lead to the formation of incomplete shells. The interactions also need to be specific enough to prevent the assembly of malformed shells, but while maintaining kinetic accessibility. Free energy landscapes computed for our model have a funnel-like topography guiding the system to form the target structure and show that the torsional component of the interparticle interactions prevents the formation of disordered aggregates that would otherwise act as kinetic traps.

Self-assembly in the ferritin nano-cage protein superfamily.

PubMed

Zhang, Yu; Orner, Brendan P

2011-01-01

Protein self-assembly, through specific, high affinity, and geometrically constraining protein-protein interactions, can control and lead to complex cellular nano-structures. Establishing an understanding of the underlying principles that govern protein self-assembly is not only essential to appreciate the fundamental biological functions of these structures, but could also provide a basis for their enhancement for nano-material applications. The ferritins are a superfamily of well studied proteins that self-assemble into hollow cage-like structures which are ubiquitously found in both prokaryotes and eukaryotes. Structural studies have revealed that many members of the ferritin family can self-assemble into nano-cages of two types. Maxi-ferritins form hollow spheres with octahedral symmetry composed of twenty-four monomers. Mini-ferritins, on the other hand, are tetrahedrally symmetric, hollow assemblies composed of twelve monomers. This review will focus on the structure of members of the ferritin superfamily, the mechanism of ferritin self-assembly and the structure-function relations of these proteins.

Mobile assemblies of Bennett linkages from four-crease origami patterns

NASA Astrophysics Data System (ADS)

Zhang, Xiao; Chen, Yan

2018-02-01

This paper deals with constructing mobile assemblies of Bennett linkages inspired by four-crease origami patterns. A transition technique has been proposed by taking the thick-panel form of an origami pattern as an intermediate bridge. A zero-thickness rigid origami pattern and its thick-panel form share the same sector angles and folding behaviours, while the thick-panel origami and the mobile assembly of linkages are kinematically equivalent with differences only in link profiles. Applying this transition technique to typical four-crease origami patterns, we have found that the Miura-ori and graded Miura-ori patterns lead to assemblies of Bennett linkages with identical link lengths. The supplementary-type origami patterns with different mountain-valley crease assignments correspond to different types of Bennett linkage assemblies with negative link lengths. And the identical linkage-type origami pattern generates a new mobile assembly. Hence, the transition technique offers a novel approach to constructing mobile assemblies of spatial linkages from origami patterns.

Mobile assemblies of Bennett linkages from four-crease origami patterns.

PubMed

Zhang, Xiao; Chen, Yan

2018-02-01

This paper deals with constructing mobile assemblies of Bennett linkages inspired by four-crease origami patterns. A transition technique has been proposed by taking the thick-panel form of an origami pattern as an intermediate bridge. A zero-thickness rigid origami pattern and its thick-panel form share the same sector angles and folding behaviours, while the thick-panel origami and the mobile assembly of linkages are kinematically equivalent with differences only in link profiles. Applying this transition technique to typical four-crease origami patterns, we have found that the Miura-ori and graded Miura-ori patterns lead to assemblies of Bennett linkages with identical link lengths. The supplementary-type origami patterns with different mountain-valley crease assignments correspond to different types of Bennett linkage assemblies with negative link lengths. And the identical linkage-type origami pattern generates a new mobile assembly. Hence, the transition technique offers a novel approach to constructing mobile assemblies of spatial linkages from origami patterns.

Numerical simulation of Composition B high explosive charge desensitization in gap test assembly after loading by precursor wave

NASA Astrophysics Data System (ADS)

Balagansky, I. A.; Stepanov, A. A.

2016-03-01

Results of numerical research into the desensitization of high explosive charges in water gap test-based experimental assemblies are presented. The experimental data are discussed, and the analysis using ANSYS AUTODYN 14.5 is provided. The desensitization phenomenon is well reproduced in numerical simulation using the JWL EOS and the Lee-Tarver kinetic equation for modeling of the initiation of heterogeneous high explosives with as well as without shock front waves. The analysis of the wave processes occurring during the initiation of the acceptor HE charge has been carried out. Peculiarities of the wave processes in the water gap test assemblies, which can influence the results of sensitivity measurement, have been studied. In particular, it has been established that precursor waves in the walls of the gap test assemblies can influence the detonation transmission distance.

Computed Tomography Measuring Inside Machines

NASA Technical Reports Server (NTRS)

Wozniak, James F.; Scudder, Henry J.; Anders, Jeffrey E.

1995-01-01

Computed tomography applied to obtain approximate measurements of radial distances from centerline of turbopump to leading edges of diffuser vanes in turbopump. Use of computed tomography has significance beyond turbopump application: example of general concept of measuring internal dimensions of assembly of parts without having to perform time-consuming task of taking assembly apart and measuring internal parts on coordinate-measuring machine.

SP-100 GES/NAT radiation shielding systems design and development testing

DOE Office of Scientific and Technical Information (OSTI.GOV)

Disney, R.K.; Kulikowski, H.D.; McGinnis, C.A.

1991-01-10

Advanced Energy Systems (AES) of Westinghouse Electric Corporation is under subcontract to the General Electric Company to supply nuclear radiation shielding components for the SP-100 Ground Engineering System (GES) Nuclear Assembly Test to be conducted at Westinghouse Hanford Company at Richland, Washington. The radiation shielding components are integral to the Nuclear Assembly Test (NAT) assembly and include prototypic and non-prototypic radiation shielding components which provide prototypic test conditions for the SP-100 reactor subsystem and reactor control subsystem components during the GES/NAT operations. W-AES is designing three radiation shield components for the NAT assembly; a prototypic Generic Flight System (GFS) shield,more » the Lower Internal Facility Shield (LIFS), and the Upper Internal Facility Shield (UIFS). This paper describes the design approach and development testing to support the design, fabrication, and assembly of these three shield components for use within the vacuum vessel of the GES/NAT. The GES/NAT shields must be designed to operate in a high vacuum which simulates space operations. The GFS shield and LIFS must provide prototypic radiation/thermal environments and mechanical interfaces for reactor system components. The NAT shields, in combination with the test facility shielding, must provide adequate radiation attenuation for overall test operations. Special design considerations account for the ground test facility effects on the prototypic GFS shield. Validation of the GFS shield design and performance will be based on detailed Monte Carlo analyses and developmental testing of design features. Full scale prototype testing of the shield subsystems is not planned.« less

Case report: heavy metal burden presenting as Bartter syndrome.

PubMed

Crinnion, Walter J; Tran, Jessica Q

2010-12-01

Maternal transfer of heavy metals during fetal development or lactation possibly contributed to the clinical manifestations of Bartter syndrome and developmental delay in the offspring. An 11-month-old child diagnosed with Bartter syndrome and failure to thrive was treated concurrently for elevated metal burden while he was undergoing standard medical interventions. Treatment with body-weight doses of meso-2,3-dimercaptosuccinic acid (DMSA) reduced the body burden of lead, beryllium, copper, mercury, and cadmium at the three- and sixth-month follow-up tests. During the course of the six-month treatment, the patient gained 2.4 kg (5.2 lb) and grew approximately 9.5 cm (3.75 in). His weight shifted from significantly below the 5th percentile in weight to within the 5th percentile, and from below the 5th to within the 10th percentile for length. The child's acquisition of lead, beryllium, and copper correspond to his mother's history of stained glass assembly and occurred during fetal development or lactation, since there were no other identifiable sources that could have contributed to the heavy metal burden. Tests for known genetic mutations leading to Bartter syndrome were all negative. This case report highlights the potential benefit of DMSA for treatment of heavy metal body burden in infants who present with Bartter syndrome.

Roles of Formin Nodes and Myosin Motor Activity in Mid1p-dependent Contractile-Ring Assembly during Fission Yeast Cytokinesis

PubMed Central

Coffman, Valerie C.; Nile, Aaron H.; Lee, I-Ju; Liu, Huayang

2009-01-01

Two prevailing models have emerged to explain the mechanism of contractile-ring assembly during cytokinesis in the fission yeast Schizosaccharomyces pombe: the spot/leading cable model and the search, capture, pull, and release (SCPR) model. We tested some of the basic assumptions of the two models. Monte Carlo simulations of the SCPR model require that the formin Cdc12p is present in >30 nodes from which actin filaments are nucleated and captured by myosin-II in neighboring nodes. The force produced by myosin motors pulls the nodes together to form a compact contractile ring. Live microscopy of cells expressing Cdc12p fluorescent fusion proteins shows for the first time that Cdc12p localizes to a broad band of 30–50 dynamic nodes, where actin filaments are nucleated in random directions. The proposed progenitor spot, essential for the spot/leading cable model, usually disappears without nucleating actin filaments. α-Actinin ain1 deletion cells form a normal contractile ring through nodes in the absence of the spot. Myosin motor activity is required to condense the nodes into a contractile ring, based on slower or absent node condensation in myo2-E1 and UCS rng3-65 mutants. Taken together, these data provide strong support for the SCPR model of contractile-ring formation in cytokinesis. PMID:19864459

Assembler (elec. equip.) 826.884, Assembler, Electrical (elec. equip.) 826.884--Technical Report on Development of USTES Aptitude Test Battery.

ERIC Educational Resources Information Center

Manpower Administration (DOL), Washington, DC. U.S. Training and Employment Service.

The United States Training and Employment Service General Aptitude Test Battery (GATB), first published in 1947, has been included in a continuing program of research to validate the tests against success in many different occupations. The GATB consists of 12 tests which measure nine aptitudes: General Learning Ability; Verbal Aptitude; Numerical…

Guarded Flat Plate Cryogenic Test Apparatus and Calorimeter

NASA Technical Reports Server (NTRS)

Fesmire, James E. (Inventor); Johnson, Wesley L. (Inventor)

2017-01-01

A test apparatus for thermal energy measurement of disk-shaped test specimens has a cold mass assembly locatable within a sealable chamber with a guard vessel having a guard chamber to receive a liquid fluid and a bottom surface to contact a cold side of a test specimen, and a test vessel having a test chamber to receive a liquid fluid and encompassed on one side by a center portion of the bottom surface shared with the guard vessel. A lateral wall assembly of the test vessel is closed by a vessel top, the lateral wall assembly comprising an outer wall and an inner wall having opposing surfaces that define a thermal break including a condensable vapor pocket to inhibit heat transfer through the lateral wall from the guard vessel to the test vessel. A warm boundary temperature surface is in thermal communication with a lower surface of the test specimen.

The Integration and Test Program of the James Webb Space Telescope

NASA Technical Reports Server (NTRS)

Kimble, Randy

2012-01-01

The James Webb Space Telescope (JWST) project has entered into a comprehensive integration and test (I&T) program that over the coming years will assemble the various elements of the observatory (the Optical Telescope Element [OTE], the Integrated Science Instrument Module [ISIM], and the Spacecraft) and verify the readiness of the integrated system for launch. The I&T program as replanned for a 2018 launch readiness date has a number of interesting features. These include a streamlined ISIM cryo-vacuum test program at Goddard Space Flight Center, a streamlined OTIS (OTE + ISIM) test program at Johnson Space Center (JSC), the addition of a second Core cryo-vacuum thermal test, the enhancement of the Pathfinder program at JSC, and enhancement of the subsystem-level testing program for the MIRI cryo-cooler. These latter activities all serve to reduce the risk heading into the end-to-end optical and thermal testing of the telescope at JSC, leading to reduced cost and schedule risk for that critical activity. We report here on the overall I&T program for JWST and on the status of the hardware and plans that support it.

Fission matrix-based Monte Carlo criticality analysis of fuel storage pools

DOE Office of Scientific and Technical Information (OSTI.GOV)

Farlotti, M.; Ecole Polytechnique, Palaiseau, F 91128; Larsen, E. W.

2013-07-01

Standard Monte Carlo transport procedures experience difficulties in solving criticality problems in fuel storage pools. Because of the strong neutron absorption between fuel assemblies, source convergence can be very slow, leading to incorrect estimates of the eigenvalue and the eigenfunction. This study examines an alternative fission matrix-based Monte Carlo transport method that takes advantage of the geometry of a storage pool to overcome this difficulty. The method uses Monte Carlo transport to build (essentially) a fission matrix, which is then used to calculate the criticality and the critical flux. This method was tested using a test code on a simplemore » problem containing 8 assemblies in a square pool. The standard Monte Carlo method gave the expected eigenfunction in 5 cases out of 10, while the fission matrix method gave the expected eigenfunction in all 10 cases. In addition, the fission matrix method provides an estimate of the error in the eigenvalue and the eigenfunction, and it allows the user to control this error by running an adequate number of cycles. Because of these advantages, the fission matrix method yields a higher confidence in the results than standard Monte Carlo. We also discuss potential improvements of the method, including the potential for variance reduction techniques. (authors)« less

Simplified failure sequence evaluation of reactor pressure vessel head corroding in-core instrumentation assembly

DOE Office of Scientific and Technical Information (OSTI.GOV)

McVicker, J.P.; Conner, J.T.; Hasrouni, P.N.

1995-11-01

In-Core Instrumentation (ICI) assemblies located on a Reactor Pressure Vessel Head have a history of boric acid leakage. The acid tends to corrode the nuts and studs which fasten the flanges of the assembly, thereby compromising the assembly`s structural integrity. This paper provides a simplified practical approach in determining the likelihood of an undetected progressing assembly stud deterioration, which would lead to a catastrophic loss of reactor coolant. The structural behavior of the In-Core Instrumentation flanged assembly is modeled using an elastic composite section assumption, with the studs transmitting tension and the pressure sealing gasket experiencing compression. Using the abovemore » technique, one can calculate the flange relative deflection and the consequential coolant loss flow rate, as well as the stress in any stud. A solved real life example develops the expected failure sequence and discusses the exigency of leak detection for safe shutdown. In the particular case of Calvert Cliffs Nuclear Power Plant (CCNPP) it is concluded that leak detection occurs before catastrophic failure of the ICI flange assembly.« less

Balancing the intermolecular forces in peptide amphiphiles for controlling self-assembly transitions.

PubMed

Buettner, C J; Wallace, A J; Ok, S; Manos, A A; Nicholl, M J; Ghosh, A; Tweedle, M F; Goldberger, J E

2017-06-21

While the influence of alkyl chain length and headgroup size on self-assembly behaviour has been well-established for simple surfactants, the rational control over the pH- and concentration-dependent self-assembly behaviour in stimuli responsive peptides remains an elusive goal. Here, we show that different amphiphilic peptides can have similar self-assembly phase diagrams, providing the relative strengths of the attractive and repulsive forces are balanced. Using palmitoyl-YYAAEEEEK(DO3A:Gd)-NH 2 and palmitoyl-YAAEEEEK(DO3A:Gd)-NH 2 as controls, we show that reducing hydrophobic attractive forces through fewer methylene groups in the alkyl chain will lead to a similar self-assembly phase diagram as increasing the electrostatic repulsive forces via the addition of a glutamic acid residue. These changes allow creation of self-assembled MRI vehicles with slightly different micelle and nanofiber diameters but with minimal changes in the spin-lattice T 1 relaxivity. These findings reveal a powerful strategy to design self-assembled vehicles with different sizes but with similar self-assembly profiles.

LPT. Shield test facility assembly and test building (TAN646). East ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

LPT. Shield test facility assembly and test building (TAN-646). East facade of ebor helium wing addition. Camera facing west. Note asbestos-cement siding on stair enclosure and upper-level. Concrete siding at lower level. Metal stack. Monorail protrudes from upper level of south wall at left of view. INEEL negative no. HD-40-7-4 - Idaho National Engineering Laboratory, Test Area North, Scoville, Butte County, ID

ATM/cable arch and beam structural test program

NASA Technical Reports Server (NTRS)

Housley, J. A.

1972-01-01

The structural testing is described of an Apollo Telescope Mount (ATM) cable arch and beam assembly, using static loads to simulate the critical conditions expected during transportation and launch of the ATM. All test objectives were met. Stress and deflection data show that the assembly is structurally adequate for use in the ATM.

Transfer orbit stage mechanisms thermal vacuum test

NASA Technical Reports Server (NTRS)

Oleary, Scott T.

1990-01-01

A systems level mechanisms test was conducted on the Orbital Sciences Corp.'s Transfer Orbit Stage (TOS). The TOS is a unique partially reusable transfer vehicle which will boost a satellite into its operational orbit from the Space Shuttle's cargo bay. The mechanical cradle and tilt assemblies will return to earth with the Space Shuttle while the Solid Rocket Motor (SRM) and avionics package are expended. A mechanisms test was performed on the forward cradle and aft tilting assemblies of the TOS under thermal vacuum conditions. Actuating these assemblies under a 1 g environment and thermal vacuum conditions proved to be a complex task. Pneumatic test fixturing was used to lift the forward cradle, and tilt the SRM, and avionics package. Clinometers, linear voltage displacement transducers, and load cells were used in the thermal vacuum chamber to measure the performance and characteristics of the TOS mechanism assembly. Incorporation of the instrumentation and pneumatic system into the test setup was not routine since pneumatic actuation of flight hardware had not been previously performed in the facility. The methods used are presented along with the problems experienced during the design, setup and test phases.

PBF Reactor Building (PER620). Fuel rod test assembly is on ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

PBF Reactor Building (PER-620). Fuel rod test assembly is on display at PBF. Date: 1982. INEEL negative no. 82-4893 - Idaho National Engineering Laboratory, SPERT-I & Power Burst Facility Area, Scoville, Butte County, ID

View of parking (resting) frame that supported the Shuttle assembly ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

View of parking (resting) frame that supported the Shuttle assembly when the hydrodynamic supports were not engaged (removed from structure). - Marshall Space Flight Center, Saturn V Dynamic Test Facility, East Test Area, Huntsville, Madison County, AL

Experimentally testing and assessing the predictive power of species assembly rules for tropical canopy ants

PubMed Central

Fayle, Tom M; Eggleton, Paul; Manica, Andrea; Yusah, Kalsum M; Foster, William A

2015-01-01

Understanding how species assemble into communities is a key goal in ecology. However, assembly rules are rarely tested experimentally, and their ability to shape real communities is poorly known. We surveyed a diverse community of epiphyte-dwelling ants and found that similar-sized species co-occurred less often than expected. Laboratory experiments demonstrated that invasion was discouraged by the presence of similarly sized resident species. The size difference for which invasion was less likely was the same as that for which wild species exhibited reduced co-occurrence. Finally we explored whether our experimentally derived assembly rules could simulate realistic communities. Communities simulated using size-based species assembly exhibited diversities closer to wild communities than those simulated using size-independent assembly, with results being sensitive to the combination of rules employed. Hence, species segregation in the wild can be driven by competitive species assembly, and this process is sufficient to generate observed species abundance distributions for tropical epiphyte-dwelling ants. PMID:25622647

Hierarchical assembly of branched supramolecular polymers from (cyclic Peptide)-polymer conjugates.

PubMed

Koh, Ming Liang; Jolliffe, Katrina A; Perrier, Sébastien

2014-11-10

We report the synthesis and assembly of (N-methylated cyclic peptide)-polymer conjugates for which the cyclic peptide is attached to either the α- or both α- and ω- end groups of a polymer. A combination of chromatographic, spectroscopic, and scattering techniques reveals that the assembly of the conjugates follows a two-level hierarchy, initially driven by H-bond formation between two N-methylated cyclic peptides, followed by unspecific, noncovalent aggregation of this peptide into small domains that behave as branching points and lead to the formation of branched supramolecular polymers.

Synthesis and Evaluation of N-phenyl-3-sulfamoyl-benzamide Derivatives as Capsid Assembly Modulators inhibiting Hepatitis B Virus (HBV).

PubMed

Vandyck, Koen; Rombouts, Geert; Stoops, Bart; Tahri, Abdellah; Vos, Ann; Verschueren, Wim; Wu, Yiming; Yang, Jingmei; Hou, Fuliang; Huang, Bing; Vergauwen, Karen; Dehertogh, Pascale; Berke, Jan-Martin; Raboisson, Pierre Jean Marie Bernard

2018-06-15

Small molecule induced Hepatitis B virus (HBV) capsid assembly modulation is considered an attractive approach for new antiviral therapies against HBV. Here we describe efforts towards the discovery of a HBV capsid assembly modulator in a hit-to-lead optimization, resulting in JNJ-632, a tool compound used to further profile the mode of action. Administration of JNJ-632 (54) in HBV genotype D infected chimeric mice, resulted in a 2.77 log reduction of the HBV DNA viral load.

Toward high throughput optical metamaterial assemblies.

PubMed

Fontana, Jake; Ratna, Banahalli R

2015-11-01

Optical metamaterials have unique engineered optical properties. These properties arise from the careful organization of plasmonic elements. Transitioning these properties from laboratory experiments to functional materials may lead to disruptive technologies for controlling light. A significant issue impeding the realization of optical metamaterial devices is the need for robust and efficient assembly strategies to govern the order of the nanometer-sized elements while enabling macroscopic throughput. This mini-review critically highlights recent approaches and challenges in creating these artificial materials. As the ability to assemble optical metamaterials improves, new unforeseen opportunities may arise for revolutionary optical devices.

Active structuring of colloidal armour on liquid drops

NASA Astrophysics Data System (ADS)

Dommersnes, Paul; Rozynek, Zbigniew; Mikkelsen, Alexander; Castberg, Rene; Kjerstad, Knut; Hersvik, Kjetil; Otto Fossum, Jon

2013-06-01

Adsorption and assembly of colloidal particles at the surface of liquid droplets are at the base of particle-stabilized emulsions and templating. Here we report that electrohydrodynamic and electro-rheological effects in leaky-dielectric liquid drops can be used to structure and dynamically control colloidal particle assemblies at drop surfaces, including electric-field-assisted convective assembly of jammed colloidal ‘ribbons’, electro-rheological colloidal chains confined to a two-dimensional surface and spinning colloidal domains on that surface. In addition, we demonstrate the size control of ‘pupil’-like openings in colloidal shells. We anticipate that electric field manipulation of colloids in leaky dielectrics can lead to new routes of colloidosome assembly and design for ‘smart armoured’ droplets.

Entropy driven key-lock assembly.

PubMed

Odriozola, G; Jiménez-Angeles, F; Lozada-Cassou, M

2008-09-21

The effective interaction between a sphere with an open cavity (lock) and a spherical macroparticle (key), both immersed in a hard sphere fluid, is studied by means of Monte Carlo simulations. As a result, a two-dimensional map of the key-lock effective interaction potential is constructed, which leads to the proposal of a self-assembling mechanism: There exists trajectories through which the key-lock pair could assemble avoiding trespassing potential barriers. Hence, solely the entropic contribution can induce their self-assembling even in the absence of attractive forces. This study points out the solvent contribution within the underlying mechanisms of substrate-protein assemblydisassembly processes, which are important steps of the enzyme catalysis and protein mediated transport.

Active structuring of colloidal armour on liquid drops.

PubMed

Dommersnes, Paul; Rozynek, Zbigniew; Mikkelsen, Alexander; Castberg, Rene; Kjerstad, Knut; Hersvik, Kjetil; Otto Fossum, Jon

2013-01-01

Adsorption and assembly of colloidal particles at the surface of liquid droplets are at the base of particle-stabilized emulsions and templating. Here we report that electrohydrodynamic and electro-rheological effects in leaky-dielectric liquid drops can be used to structure and dynamically control colloidal particle assemblies at drop surfaces, including electric-field-assisted convective assembly of jammed colloidal 'ribbons', electro-rheological colloidal chains confined to a two-dimensional surface and spinning colloidal domains on that surface. In addition, we demonstrate the size control of 'pupil'-like openings in colloidal shells. We anticipate that electric field manipulation of colloids in leaky dielectrics can lead to new routes of colloidosome assembly and design for 'smart armoured' droplets.

KSC-2009-3673

NASA Image and Video Library

2009-06-11

CAPE CANAVERAL, Fla. – At the Assembly and Refurbishment Facility at NASA's Kennedy Space Center in Florida, Robert Lightfoot, acting center director of NASA's Marshall Space Flight Center, speaks to employees who were involved in the processing of the Ares I-X forward assembly (comprising the frustum, forward skirt extension and forward skirt) . The forward assembly is being moved to the Vehicle Assembly Building's High Bay 4 for processing and stacking to the upper stage. Ares I-X is the flight test for the Ares I which will provide NASA an early opportunity to test and prove hardware, facilities and ground operations associated with Ares I, which is part of the Constellation Program to return men to the moon and beyond. Launch of the Ares I-X flight test is targeted for August 2009. Photo credit: NASA/Jack Pfaller

Special test equipment and fixturing for MSAT reflector assembly alignment

NASA Technical Reports Server (NTRS)

Young, Jeffrey A.; Zinn, Michael R.; Mccarten, David R.

1994-01-01

The MSAT Reflector Assembly is a state of the art subsystem for Mobile Satellite (MSAT), a geosynchronous-based commercial mobile telecommunication satellite program serving North America. The Reflector Assembly consisted of a deployable, three-hinge, folding-segment Boom, deployable 5.7 x 5.3-meter 16-rib Wrap-Rib Reflector, and a Reflector Pointing Mechanism (RPM). The MSAT spacecraft was based on a Hughes HS601 spacecraft bus carrying two Reflector Assemblies independently dedicated for L-band transmit and receive operations. Lockheed Missiles and Space Company (LMSC) designed and built the Reflector Assembly for MSAT under contract to SPAR Aerospace Ltd. Two MSAT satellites were built jointly by SPAR Aerospace Ltd. and Hughes Space and Communications Co. for this program, the first scheduled for launch in 1994. When scaled for wavelength, the assembly and alignment requirements for the Reflector Assembly were in many instances equivalent to or exceeded that of a diffraction-limited visible light optical system. Combined with logistical constraints inherent to large, compliant, lightweight structures; 'bolt-on' alignment; and remote, indirect spacecraft access; the technical challenges were formidable. This document describes the alignment methods, the special test equipment, and fixturing for Reflector Assembly assembly and alignment.

CHP Integrated with Burners for Packaged Boilers

DOE Office of Scientific and Technical Information (OSTI.GOV)

Castaldini, Carlo; Darby, Eric

2013-09-30

The objective of this project was to engineer, design, fabricate, and field demonstrate a Boiler Burner Energy System Technology (BBEST) that integrates a low-cost, clean burning, gas-fired simple-cycle (unrecuperated) 100 kWe (net) microturbine (SCMT) with a new ultra low-NOx gas-fired burner (ULNB) into one compact Combined Heat and Power (CHP) product that can be retrofit on new and existing industrial and commercial boilers in place of conventional burners. The Scope of Work for this project was segmented into two principal phases: (Phase I) Hardware development, assembly and pre-test and (Phase II) Field installation and demonstration testing. Phase I was dividedmore » into five technical tasks (Task 2 to 6). These tasks covered the engineering, design, fabrication, testing and optimization of each key component of the CHP system principally, ULNB, SCMT, assembly BBEST CHP package, and integrated controls. Phase I work culminated with the laboratory testing of the completed BBEST assembly prior to shipment for field installation and demonstration. Phase II consisted of two remaining technical tasks (Task 7 and 8), which focused on the installation, startup, and field verification tests at a pre-selected industrial plant to document performance and attainment of all project objectives. Technical direction and administration was under the management of CMCE, Inc. Altex Technologies Corporation lead the design, assembly and testing of the system. Field demonstration was supported by Leva Energy, the commercialization firm founded by executives at CMCE and Altex. Leva Energy has applied for patent protection on the BBEST process under the trade name of Power Burner and holds the license for the burner currently used in the product. The commercial term Power Burner is used throughout this report to refer to the BBEST technology proposed for this project. The project was co-funded by the California Energy Commission and the Southern California Gas Company (SCG), a division of Sempra Energy. These match funds were provided via concurrent contracts and investments available via CMCE, Altex, and Leva Energy The project attained all its objectives and is considered a success. CMCE secured the support of GI&E from Italy to supply 100 kW Turbec T-100 microturbines for the project. One was purchased by the project’s subcontractor, Altex, and a second spare was purchased by CMCE under this project. The microturbines were then modified to convert from their original recuperated design to a simple cycle configuration. Replacement low-NOx silo combustors were designed and bench tested in order to achieve compliance with the California Air Resources Board (CARB) 2007 emission limits for NOx and CO when in CHP operation. The converted microturbine was then mated with a low NOx burner provided by Altex via an integration section that allowed flow control and heat recovery to minimize combustion blower requirements; manage burner turndown; and recover waste heat. A new fully integrated control system was designed and developed that allowed one-touch system operation in all three available modes of operation: (1) CHP with both microturbine and burner firing for boiler heat input greater than 2 MMBtu/hr; (2) burner head only (BHO) when the microturbine is under service; and (3) microturbine only when boiler heat input requirements fall below 2 MMBtu/hr. This capability resulted in a burner turndown performance of nearly 10/1, a key advantage for this technology over conventional low NOx burners. Key components were then assembled into a cabinet with additional support systems for generator cooling and fuel supply. System checkout and performance tests were performed in the laboratory. The assembled system and its support equipment were then shipped and installed at a host facility where final performance tests were conducted following efforts to secure fabrication, air, and operating permits. The installed power burner is now in commercial operation and has achieved all the performance goals.« less

89. Neg. No. F126, Jul 21, 1931, INTERIORASSEMBLY BUILDING, SHOWING ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

89. Neg. No. F-126, Jul 21, 1931, INTERIOR-ASSEMBLY BUILDING, SHOWING HEADLIGHT TESTING BOOTH - Ford Motor Company Long Beach Assembly Plant, Assembly Building, 700 Henry Ford Avenue, Long Beach, Los Angeles County, CA

Structure Assembly by a Heterogeneous Team of Robots Using State Estimation, Generalized Joints, and Mobile Parallel Manipulators

NASA Technical Reports Server (NTRS)

Komendera, Erik E.; Adhikari, Shaurav; Glassner, Samantha; Kishen, Ashwin; Quartaro, Amy

2017-01-01

Autonomous robotic assembly by mobile field robots has seen significant advances in recent decades, yet practicality remains elusive. Identified challenges include better use of state estimation to and reasoning with uncertainty, spreading out tasks to specialized robots, and implementing representative joining methods. This paper proposes replacing 1) self-correcting mechanical linkages with generalized joints for improved applicability, 2) assembly serial manipulators with parallel manipulators for higher precision and stability, and 3) all-in-one robots with a heterogeneous team of specialized robots for agent simplicity. This paper then describes a general assembly algorithm utilizing state estimation. Finally, these concepts are tested in the context of solar array assembly, requiring a team of robots to assemble, bond, and deploy a set of solar panel mockups to a backbone truss to an accuracy not built into the parts. This paper presents the results of these tests.

Necklace: combining reference and assembled transcriptomes for more comprehensive RNA-Seq analysis.

PubMed

Davidson, Nadia M; Oshlack, Alicia

2018-05-01

RNA sequencing (RNA-seq) analyses can benefit from performing a genome-guided and de novo assembly, in particular for species where the reference genome or the annotation is incomplete. However, tools for integrating an assembled transcriptome with reference annotation are lacking. Necklace is a software pipeline that runs genome-guided and de novo assembly and combines the resulting transcriptomes with reference genome annotations. Necklace constructs a compact but comprehensive superTranscriptome out of the assembled and reference data. Reads are subsequently aligned and counted in preparation for differential expression testing. Necklace allows a comprehensive transcriptome to be built from a combination of assembled and annotated transcripts, which results in a more comprehensive transcriptome for the majority of organisms. In addition RNA-seq data are mapped back to this newly created superTranscript reference to enable differential expression testing with standard methods.

Composite components under impact load and effects of defects on the loading capacity. [Alpha Jet tail assembly

NASA Technical Reports Server (NTRS)

Aoki, R.; Wurzel, D.

1979-01-01

Investigations were carried out on a horizontal tail assembly made of carbon fiber reinforced plastic for the Alpha Jet. The possibility of obtaining a leading edge nose design lighter but not more expensive than a metal version was studied. An important consideration was sufficient resistance of the leading edge against impact of stones and hailstones combined with high degree of stiffness. The improvement of energy reception characteristics of the materials through suitable laminate design was considered. Since certain defects occur in structural components, the effects of such defects on the characteristics of the parts were also studied.

Leaf seal for gas turbine stator shrouds and a nozzle band

DOEpatents

Burdgick, Steven Sebastian; Sexton, Brendan Francis

2002-01-01

A leaf seal assembly is secured to the trailing edge of a shroud segment for sealing between the shroud segment and the leading edge side wall of a nozzle outer band. The leaf seal includes a circumferentially elongated seal plate biased by a pair of spring clips disposed in a groove along the trailing edge of the shroud segment to maintain the seal plate in engagement with the flange on the leading edge side wall of the nozzle outer band. The leaf seal plate and spring clips receive pins tack-welded to the shroud segment to secure the leaf seal assembly in place.

Development Specification for the Feedwater Supply Assembly (FSA), FSA-431 with Integrated Auxiliary Feedwater Supply Assembly (AFSA), FSA-531

NASA Technical Reports Server (NTRS)

Anchondo, Ian; Campbell, Colin

2017-01-01

The FSA with Integrated Aux FSA Specification establishes the requirements for design, performance, and testing of the FSA-431/FSA-531 assembly in compliance with CTSD-ADV-780, Development Specification for the Advanced EMU (AEMU) Portable Life Support System (PLSS). This section contains the technical design and performance requirements for the integrated assembly of the Feedwater Supply Assembly and Auxiliary Feedwater Supply Assembly for the Advanced EVA Development Portable Life Support Subsystem (PLSS).

Integrated Advanced Microwave Sounding Unit-A (AMSU-A). Performance Verification Report: AMSU-A1 Antenna Drive Subsystem, PN 1331720-2, S/N 106

NASA Technical Reports Server (NTRS)

Luu, D.

1999-01-01

This is the Performance Verification Report, AMSU-A1 Antenna Drive Subsystem, P/N 1331720-2, S/N 106, for the Integrated Advanced Microwave Sounding Unit-A (AMSU-A). The antenna drive subsystem of the METSAT AMSU-A1, S/N 106, P/N 1331720-2, completed acceptance testing per A-ES Test Procedure AE-26002/lD. The test included: Scan Motion and Jitter, Pulse Load Bus Peak Current and Rise Time, Resolver Reading and Position Error, Gain/ Phase Margin, and Operational Gain Margin. The drive motors and electronic circuitry were also tested at the component level. The drive motor test includes: Starting Torque Test, Motor Commutation Test, Resolver Operation/ No-Load Speed Test, and Random Vibration. The electronic circuitry was tested at the Circuit Card Assembly (CCA) level of production; each test exercised all circuit functions. The transistor assembly was tested during the W3 cable assembly (1356941-1) test.

Automated ensemble assembly and validation of microbial genomes.

PubMed

Koren, Sergey; Treangen, Todd J; Hill, Christopher M; Pop, Mihai; Phillippy, Adam M

2014-05-03

The continued democratization of DNA sequencing has sparked a new wave of development of genome assembly and assembly validation methods. As individual research labs, rather than centralized centers, begin to sequence the majority of new genomes, it is important to establish best practices for genome assembly. However, recent evaluations such as GAGE and the Assemblathon have concluded that there is no single best approach to genome assembly. Instead, it is preferable to generate multiple assemblies and validate them to determine which is most useful for the desired analysis; this is a labor-intensive process that is often impossible or unfeasible. To encourage best practices supported by the community, we present iMetAMOS, an automated ensemble assembly pipeline; iMetAMOS encapsulates the process of running, validating, and selecting a single assembly from multiple assemblies. iMetAMOS packages several leading open-source tools into a single binary that automates parameter selection and execution of multiple assemblers, scores the resulting assemblies based on multiple validation metrics, and annotates the assemblies for genes and contaminants. We demonstrate the utility of the ensemble process on 225 previously unassembled Mycobacterium tuberculosis genomes as well as a Rhodobacter sphaeroides benchmark dataset. On these real data, iMetAMOS reliably produces validated assemblies and identifies potential contamination without user intervention. In addition, intelligent parameter selection produces assemblies of R. sphaeroides comparable to or exceeding the quality of those from the GAGE-B evaluation, affecting the relative ranking of some assemblers. Ensemble assembly with iMetAMOS provides users with multiple, validated assemblies for each genome. Although computationally limited to small or mid-sized genomes, this approach is the most effective and reproducible means for generating high-quality assemblies and enables users to select an assembly best tailored to their specific needs.

Quality control of FWC during assembly and commissioning in SST-1 Tokamak

NASA Astrophysics Data System (ADS)

Patel, Hitesh; Santra, Prosenjit; Parekh, Tejas; Biswas, Prabal; Jayswal, Snehal; Chauhan, Pradeep; Paravastu, Yuvakiran; George, Siju; Semwal, Pratibha; Thankey, Prashant; Ramesh, Gattu; Prakash, Arun; Dhanani, Kalpesh; Raval, D. C.; Khan, Ziauddin; Pradhan, Subrata

2017-04-01

First Wall Components (FWC) of SST-1 tokamak, which are in the immediate vicinity of plasma, comprises of limiters, divertors, baffles, passive stabilizers designed to operate long duration (∼1000 s) discharges of elongated plasma. All FWC consist of copper alloy heat sink modules with SS cooling tubes brazed onto it, graphite tiles acting as armour material facing the plasma, and are mounted to the vacuum vessels with suitable Inconel support structures at inter-connected ring & port locations. The FWC are very recently assembled and commissioned successfully inside the vacuum vessel of SST-1 undergoing a rigorous quality control and checks at every stage of the assembly process. This paper will present the quality control aspects and checks of FWC from commencement of assembly procedure, namely material test reports, leak testing of high temperature baked components, assembled dimensional tolerances, leak testing of all welded joints, graphite tile tightening torques, electrical continuity and electrical isolation of passive stabilizers from vacuum vessel, baking and cooling hydraulic connections inside vacuum vessel.

Dependence of Halo Bias and Kinematics on Assembly Variables

NASA Astrophysics Data System (ADS)

Xu, Xiaoju; Zheng, Zheng

2018-06-01

Using dark matter haloes identified in a large N-body simulation, we study halo assembly bias, with halo formation time, peak maximum circular velocity, concentration, and spin as the assembly variables. Instead of grouping haloes at fixed mass into different percentiles of each assembly variable, we present the joint dependence of halo bias on the values of halo mass and each assembly variable. In the plane of halo mass and one assembly variable, the joint dependence can be largely described as halo bias increasing outward from a global minimum. We find it unlikely to have a combination of halo variables to absorb all assembly bias effects. We then present the joint dependence of halo bias on two assembly variables at fixed halo mass. The gradient of halo bias does not necessarily follow the correlation direction of the two assembly variables and it varies with halo mass. Therefore in general for two correlated assembly variables one cannot be used as a proxy for the other in predicting halo assembly bias trend. Finally, halo assembly is found to affect the kinematics of haloes. Low-mass haloes formed earlier can have much higher pairwise velocity dispersion than those of massive haloes. In general, halo assembly leads to a correlation between halo bias and halo pairwise velocity distribution, with more strongly clustered haloes having higher pairwise velocity and velocity dispersion. However, the correlation is not tight, and the kinematics of haloes at fixed halo bias still depends on halo mass and assembly variables.

Runflat Testing

DTIC Science & Technology

2015-09-09

guidance and procedures for testing the performance characteristics of runflat tires as equipped on ground vehicles. 15. SUBJECT TERMS runflat... tire assembly tread life combat flat central tire inflation system (CTIS) 16. SECURITY CLASSIFICATION OF: 17. LIMITATION...3 3.4 Tire Assemblies ..................................................................... 3 3.5 Environmental

Physical principles for DNA tile self-assembly.

PubMed

Evans, Constantine G; Winfree, Erik

2017-06-19

DNA tiles provide a promising technique for assembling structures with nanoscale resolution through self-assembly by basic interactions rather than top-down assembly of individual structures. Tile systems can be programmed to grow based on logical rules, allowing for a small number of tile types to assemble large, complex assemblies that can retain nanoscale resolution. Such algorithmic systems can even assemble different structures using the same tiles, based on inputs that seed the growth. While programming and theoretical analysis of tile self-assembly often makes use of abstract logical models of growth, experimentally implemented systems are governed by nanoscale physical processes that can lead to very different behavior, more accurately modeled by taking into account the thermodynamics and kinetics of tile attachment and detachment in solution. This review discusses the relationships between more abstract and more physically realistic tile assembly models. A central concern is how consideration of model differences enables the design of tile systems that robustly exhibit the desired abstract behavior in realistic physical models and in experimental implementations. Conversely, we identify situations where self-assembly in abstract models can not be well-approximated by physically realistic models, putting constraints on physical relevance of the abstract models. To facilitate the discussion, we introduce a unified model of tile self-assembly that clarifies the relationships between several well-studied models in the literature. Throughout, we highlight open questions regarding the physical principles for DNA tile self-assembly.