Science.gov

Sample records for lensless digital holography

  1. Surpassing digital holography limits by lensless object scanning holography.

    PubMed

    Micó, Vicente; Ferreira, Carlos; García, Javier

    2012-04-23

    We present lensless object scanning holography (LOSH) as a fully lensless method, capable of improving image quality in reflective digital Fourier holography, by means of an extremely simplified experimental setup. LOSH is based on the recording and digital post-processing of a set of digital lensless holograms and results in a synthetic image with improved resolution, field of view (FOV), signal-to-noise ratio (SNR), and depth of field (DOF). The superresolution (SR) effect arises from the generation of a synthetic aperture (SA) based on the linear movement of the inspected object. The same scanning principle enlarges the object FOV. SNR enhancement is achieved by speckle suppression and coherent artifacts averaging due to the coherent addition of the multiple partially overlapping bandpass images. And DOF extension is performed by digital refocusing to different object's sections. Experimental results showing an impressive image quality improvement are reported for a one-dimensional reflective resolution test target.

  2. On-chip differential interference contrast microscopy using lensless digital holography.

    PubMed

    Oh, Chulwoo; Isikman, Serhan O; Khademhosseinieh, Bahar; Ozcan, Aydogan

    2010-03-01

    We introduce the use of a birefringent crystal with lensless digital holography to create an on-chip differential interference contrast (DIC) microscope. Using an incoherent source with a large aperture, in-line holograms of micro-objects are created, which interact with a uniaxial crystal and an absorbing polarizer, encoding differential interference contrast information of the objects on the chip. Despite the fact that a unit fringe magnification and an incoherent source with a large aperture have been used, holographic digital processing of such holograms rapidly recovers the differential phase contrast image of the specimen over a large field-of-view of approximately 24 mm(2).

  3. Non-invasive monitoring for living cell culture with lensless Fourier transform digital holography microscopy

    NASA Astrophysics Data System (ADS)

    Wang, Yunxin; Wang, Dayong; Zhao, Jie; Li, Yan; Meng, Puhui; Wan, Yuhong; Jiang, Zhuqing

    2010-08-01

    The number of cells is commonly employed to describe the cell viability and the status of cell culture in a certain extent. An automatic and non-invasive detecting method for the status analysis of cell culture is developed based on digital holography microscopy (DHM) technology. Digital holographic imaging can retrieve quantitative information of object wavefront by the numerical reconstruction from a single digital hologram recorded by a detector such as CCD or CMOS camera, which is especially suitable for the morphology detection of the transparent or semi-transparent cells. In this contribution, the lensless Fourier transform (LFT) based holography configuration is designed for cell imaging without prestaining, and the amplitude and phase of living cells can be reconstructed by digital reconstruction and phase unwrapped algorithms. Then the image filtering and segmentation are combined for the automatic evaluation of the level of confluency. In imaging experiments, the culture status of the cervical cancer cell TZMbl is detected, and the results demonstrate that digital holography microscopy provides a feasible non-invasive method for monitoring the living cell culture.

  4. Speckle suppression in off-axis lensless Fourier transform digital holography

    NASA Astrophysics Data System (ADS)

    Panezai, Spozmai; Zhao, Jie; Wang, Yunxin; Wang, Dayong; Rong, Lu

    2017-08-01

    Speckle noise suppression in off-axis lensless Fourier transform digital holography by laterally shifting of object is analyzed quantitatively. Speckle decorrelation is directly proportional to the object displacement therefore object is shifted by three different amounts to test its effect on the speckle contrast reduction that is pixel size of camera, averaged speckle size and resolution pixel size of reconstruction plane. Phase-only spatial light modulator (LCOS) is used in object beam path to introduce the lateral shift in its position digitally without mechanical efforts by displaying a grating function. In comparison with the typical methods, the externally input lateral position shifts of object are controlled accurately, which makes the system effective and practicable. Averaged reconstructed results for three quantitative object lateral position shifts are compared and it has been found that the object shift by resolution pixel size of reconstruction plane shows better speckle contrast reduction and is in good agreement with the theoretical prediction.

  5. Multi-angle lensless digital holography for depth resolved imaging on a chip.

    PubMed

    Su, Ting-Wei; Isikman, Serhan O; Bishara, Waheb; Tseng, Derek; Erlinger, Anthony; Ozcan, Aydogan

    2010-04-26

    A multi-angle lensfree holographic imaging platform that can accurately characterize both the axial and lateral positions of cells located within multi-layered micro-channels is introduced. In this platform, lensfree digital holograms of the micro-objects on the chip are recorded at different illumination angles using partially coherent illumination. These digital holograms start to shift laterally on the sensor plane as the illumination angle of the source is tilted. Since the exact amount of this lateral shift of each object hologram can be calculated with an accuracy that beats the diffraction limit of light, the height of each cell from the substrate can be determined over a large field of view without the use of any lenses. We demonstrate the proof of concept of this multi-angle lensless imaging platform by using light emitting diodes to characterize various sized microparticles located on a chip with sub-micron axial and lateral localization over approximately 60 mm(2) field of view. Furthermore, we successfully apply this lensless imaging approach to simultaneously characterize blood samples located at multi-layered micro-channels in terms of the counts, individual thicknesses and the volumes of the cells at each layer. Because this platform does not require any lenses, lasers or other bulky optical/mechanical components, it provides a compact and high-throughput alternative to conventional approaches for cytometry and diagnostics applications involving lab on a chip systems.

  6. Multi-angle lensless digital holography for depth resolved imaging on a chip

    PubMed Central

    Su, Ting-Wei; Isikman, Serhan O.; Bishara, Waheb; Tseng, Derek; Erlinger, Anthony; Ozcan, Aydogan

    2010-01-01

    A multi-angle lensfree holographic imaging platform that can accurately characterize both the axial and lateral positions of cells located within multi-layered micro-channels is introduced. In this platform, lensfree digital holograms of the micro-objects on the chip are recorded at different illumination angles using partially coherent illumination. These digital holograms start to shift laterally on the sensor plane as the illumination angle of the source is tilted. Since the exact amount of this lateral shift of each object hologram can be calculated with an accuracy that beats the diffraction limit of light, the height of each cell from the substrate can be determined over a large field of view without the use of any lenses. We demonstrate the proof of concept of this multi-angle lensless imaging platform by using light emitting diodes to characterize various sized microparticles located on a chip with sub-micron axial and lateral localization over ~60 mm2 field of view. Furthermore, we successfully apply this lensless imaging approach to simultaneously characterize blood samples located at multi-layered micro-channels in terms of the counts, individual thicknesses and the volumes of the cells at each layer. Because this platform does not require any lenses, lasers or other bulky optical/mechanical components, it provides a compact and high-throughput alternative to conventional approaches for cytometry and diagnostics applications involving lab on a chip systems. PMID:20588819

  7. Superresolution imaging method using phase-shifting digital lensless Fourier holography.

    PubMed

    Granero, Luis; Micó, Vicente; Zalevsky, Zeev; García, Javier

    2009-08-17

    A method which is useful for obtaining superresolved imaging in a digital lensless Fourier holographic configuration is presented. By placing a diffraction grating between the input object and the CCD recording device, additional high-order spatial-frequency content of the object spectrum is directed towards the CCD. Unlike other similar methods, the recovery of the different band pass images is performed by inserting a reference beam in on-axis mode and using phase-shifting method. This strategy provides advantages concerning the usage of the whole frequency plane as imaging plane. Thus, the method is no longer limited by the zero order term and the twin image. Finally, the whole process results in a synthetic aperture generation that expands up the system cutoff frequency and yields a superresolution effect. Experimental results validate our concepts for a resolution improvement factor of 3. (c) 2009 Optical Society of America

  8. Synthetic aperture engineering for super-resolved microscopy in digital lensless Fourier holography

    NASA Astrophysics Data System (ADS)

    Micó, Vicente; Granero, Luis; Zalevsky, Zeev; García, Javier

    2011-05-01

    We present a method capable to improve the resolution limit of an imaging system in digital lensless Fourier holographic configuration. The method is based on angular- and time-multiplexing of the object's spatial frequency information. On one hand, angular multiplexing is implemented by using tilted beam illumination to get access to high order spectral frequency bands of the of the object's spectrum. And, on the other hand, time multiplexing is needed to cover different directions at the spatial frequency domain. This combination of angular- and time- multiplexing in addition with holographic recording allows the complex amplitude recovery of a set of elementary apertures covering different portions of the object's spectrum. Finally, the expanded synthetic aperture (SA) is generated by coherent addition of the set of recovered elementary apertures. Such SA expands up the cut-off frequency limit of the imaging system and allows getting a superresolved image of the input object. Moreover, if a priori knowledge about the input object is available, customized SA shaping is possible by considering the addition of those elementary apertures corresponding with only the directions of interest and, thus, reducing the whole consuming time of the approach. We present experimental results in concordance with theoretical predictions for two different resolution test objects, for different SA shapes, and considering different resolution gain factors.

  9. Lensless digital holography with diffuse illumination through a pseudo-random phase mask.

    PubMed

    Bernet, Stefan; Harm, Walter; Jesacher, Alexander; Ritsch-Marte, Monika

    2011-12-05

    Microscopic imaging with a setup consisting of a pseudo-random phase mask, and an open CMOS camera, without an imaging objective, is demonstrated. The pseudo random phase mask acts as a diffuser for an incoming laser beam, scattering a speckle pattern to a CMOS chip, which is recorded once as a reference. A sample which is afterwards inserted somewhere in the optical beam path changes the speckle pattern. A single (non-iterative) image processing step, comparing the modified speckle pattern with the previously recorded one, generates a sharp image of the sample. After a first calibration the method works in real-time and allows quantitative imaging of complex (amplitude and phase) samples in an extended three-dimensional volume. Since no lenses are used, the method is free from lens abberations. Compared to standard inline holography the diffuse sample illumination improves the axial sectioning capability by increasing the effective numerical aperture in the illumination path, and it suppresses the undesired so-called twin images. For demonstration, a high resolution spatial light modulator (SLM) is programmed to act as the pseudo-random phase mask. We show experimental results, imaging microscopic biological samples, e.g. insects, within an extended volume at a distance of 15 cm with a transverse and longitudinal resolution of about 60 μm and 400 μm, respectively.

  10. Synthetic aperture superresolved microscopy in digital lensless Fourier holography by time and angular multiplexing of the object information.

    PubMed

    Granero, Luis; Micó, Vicente; Zalevsky, Zeev; García, Javier

    2010-02-10

    The resolving power of an imaging system in digital lensless Fourier holographic configuration is mainly limited by the numerical aperture of the experimental setup that is defined by both the restricted CCD size and the presence of a beam splitter cube in front of the CCD. We present a method capable of improving the resolution in such a system configuration based on synthetic aperture (SA) generation by using time-multiplexing tilted illumination onto the input object. Moreover, a priori knowledge about the imaged object allows customized SA shaping by the addition of elementary apertures only in the directions of interest. Experimental results are provided, showing agreement with theoretical predictions and demonstrating a resolution limit corresponding with a synthetic numerical aperture value of 0.45.

  11. RGB digital lensless holographic microscopy

    NASA Astrophysics Data System (ADS)

    Garcia-Sucerquia, Jorge

    2013-11-01

    The recent introduction of color digital lensless holographic microscopy (CDLHM) has shown the possibility of imaging microscopic specimens at full color without the need of lenses. Owing to the simplicity, robustness, and compactness of the digital lensless holographic microscopes (DLHM), they have been presented as the ideal candidates to being developed into portable holographic microscopes. However, in the case of CDLHM the utilization of three independent lasers hinders the portability option for this microscope. In this contribution an alternative to reduce the complexity of CDLHM aimed to recover the portability of this microscopy technology is presented. A super-bright white-light light-emitting diode (LED) is spectrally and spatially filtered to produce the needed illumination by CDLHM to work. CDLHM with LED illumination is used to image at full color a section of the head of a drosophila melanogaster fly (fruit fly). The LED-CDLHM method shows the capability of imaging objects of 2μm size in comparison with the micrometer resolution reported for LASER-CDLHM.

  12. Frequency analysis of digital holography

    NASA Astrophysics Data System (ADS)

    Kreis, Thomas M.

    2002-04-01

    A coherent optical imaging system consisting of recording a digital hologram by a CCD array and numerical reconstruction of the complex wave field in a computer is subjected to a frequency analysis. This analysis recognizes Fresnel and lensless Fourier transform holography; collimated and divergent reference waves; as well as the real image, the virtual image, and the dc term. The influences of finite sampling and the fill factor of the CCD pixels are examined. The impulse response of the system is a shifted Fraunhofer diffraction pattern of the aperture defined by the CCD. A fill factor below unity leads to a contrast decrease, which is quantitatively described in the modulation transfer function.

  13. Field-portable reflection and transmission microscopy based on lensless holography.

    PubMed

    Lee, Myungjun; Yaglidere, Oguzhan; Ozcan, Aydogan

    2011-09-01

    We demonstrate a lensfree dual-mode holographic microscope that can image specimens in both transmission and reflection geometries using in-line transmission and off-axis reflection holography, respectively. This field-portable dual-mode holographic microscope has a weight of ~200 g with dimensions of 15 x 5.5 x 5cm, where a laser source is powered by two batteries. Based on digital in-line holography, our transmission microscope achieves a sub-pixel lateral resolution of ≤2 µm over a wide field-of-view (FOV) of ~24 mm(2) due to its unit fringe magnification geometry. Despite its simplicity and ease of operation, in-line transmission geometry is not suitable to image dense or connected objects such as tissue slides since the reference beam gets distorted causing severe aberrations in reconstruction of such objects. To mitigate this challenge, on the same cost-effective and field-portable assembly we built a lensless reflection mode microscope based on digital off-axis holography where a beam-splitter is used to interfere a tilted reference wave with the reflected light from the object surface, creating an off-axis hologram of the specimens on a CMOS sensor-chip. As a result of the reduced space-bandwidth product of the off-axis geometry compared to its in-line counterpart, the imaging FOV of our reflection mode is reduced to ~9 mm(2), while still achieving a similar sub-pixel resolution of ≤2 µm. We tested the performance of this compact dual-mode microscopy unit by imaging a US-air force resolution test target, various micro-particles as well as a histopathology slide corresponding to skin tissue. Due to its compact, cost-effective, and lightweight design, this dual-mode lensless holographic microscope might especially be useful for field-use or for conducting microscopic analysis in resource-poor settings.

  14. Predictive dynamic digital holography

    NASA Astrophysics Data System (ADS)

    Sulaiman, Sennan; Gibson, Steve; Spencer, Mark

    2016-09-01

    Digital holography has received recent attention for many imaging and sensing applications, including imaging through turbulent and turbid media, adaptive optics, three dimensional projective display technology and optical tweezing. A significant obstacle for digital holography in real-time applications, such as wavefront sensing for high energy laser systems and high speed imaging for target tracking, is the fact that digital holography is computationally intensive; it requires iterative virtual wavefront propagation and hill-climbing to optimize some sharpness criteria. This paper demonstrates real-time methods for digital holography based on approaches developed recently at UCLA for optimal and adaptive identification, prediction, and control of optical wavefronts. The methods presented integrate minimum variance wavefront prediction into digital holography schemes to short-circuit the computationally intensive algorithms for iterative propagation of virtual wavefronts and hill climbing for sharpness optimization.

  15. Quantitative phase imaging by wide field lensless digital holographic microscope

    NASA Astrophysics Data System (ADS)

    Adinda-Ougba, A.; Koukourakis, N.; Essaidi, A.; Ger­hardt, N. C.; Hofmann, M. R.

    2015-05-01

    Wide field, lensless microscopes have been developed for telemedicine and for resource limited setting [1]. They are based on in-line digital holography which is capable to provide amplitude and phase information resulting from numerical reconstruction. The phase information enables achieving axial resolution in the nanometer range. Hence, such microscopes provide a powerful tool to determine three-dimensional topologies of microstructures. In this contribution, a compact, low-cost, wide field, lensless microscope is presented, which is capable of providing topological profiles of microstructures in transparent material. Our setup consist only of two main components: a CMOSsensor chip and a laser diode without any need of a pinhole. We use this very simple setup to record holograms of microobjects. A wide field of view of ~24 mm², and a lateral resolution of ~2 μm are achieved. Moreover, amplitude and phase information are obtained from the numerical reconstruction of the holograms using a phase retrieval algorithm together with the angular spectrum propagation method. Topographic information of highly transparent micro-objects is obtained from the phase data. We evaluate our system by recording holograms of lines with different depths written by a focused laser beam. A reliable characterization of laser written microstructures is crucial for their functionality. Our results show that this system is valuable for determination of topological profiles of microstructures in transparent material.

  16. Multispectral lensless digital in-line holographic microscope: LED illumination

    NASA Astrophysics Data System (ADS)

    Ryle, James P.; McDonnell, Susan; Sheridan, John T.

    2010-05-01

    Holography is the process where two coherent wavefields interfere resulting in an interference pattern from which whole field information can be retrieved. Digital holography is the process where the intensity of the superposition of the two waves is recorded using a light sensitive opto-electronic detector array such as a CCD or CMOS camera. From this recorded hologram it is possible to numerically reconstruct the object wavefield. When an optical beam is focused on a pinhole whose diameter is of the order of a few times the wavelength of the illumination beam, a spherically divergent wavefield is emitted. We use the emitted optical beam to illuminate weakly scattering objects resulting in a geometrically magnified diffraction pattern at the camera face. Scattered light from the object is the called the object wavefield, while unscattered light acts as the reference wavefield. The hologram is captured digitally before numerical reconstruction is applied to yield whole field information about the object. It is possible to reconstruct the objects wavefield using this method from coherent laser or incoherent LED illumination. The emitted light from the pinhole acts a pointsource of spatially coherent light enabling holography. This, in combination with the use of multiple wavelength LED's multispectral amplitude images can be reconstructed. The multispectral lensless DIHM described here can be used to holographically image biological specimens such as cells grown for use in the biopharmaceutical industry or for research purposes. In analysing cell viability based on the trypan blue assay, the outer membrane of non-viable cells is penetrated by violet blue dye. Using such a Digital In-line Holographic Microscope as described here, automatic classification of viable and non-viable cells could be performed.

  17. A scheme for lensless X-ray microscopy combining coherent diffraction imaging and differential corner holography.

    PubMed

    Capotondi, F; Pedersoli, E; Kiskinova, M; Martin, A V; Barthelmess, M; Chapman, H N

    2012-10-22

    We successfully use the corners of a common silicon nitride supporting window in lensless X-ray microscopy as extended references in differential holography to obtain a real space hologram of the illuminated object. Moreover, we combine this method with the iterative phasing techniques of coherent diffraction imaging to enhance the spatial resolution on the reconstructed object, and overcome the problem of missing areas in the collected data due to the presence of a beam stop, achieving a resolution close to 85 nm.

  18. Compact, light-weight and cost-effective microscope based on lensless incoherent holography for telemedicine applications.

    PubMed

    Mudanyali, Onur; Tseng, Derek; Oh, Chulwoo; Isikman, Serhan O; Sencan, Ikbal; Bishara, Waheb; Oztoprak, Cetin; Seo, Sungkyu; Khademhosseini, Bahar; Ozcan, Aydogan

    2010-06-07

    Despite the rapid progress in optical imaging, most of the advanced microscopy modalities still require complex and costly set-ups that unfortunately limit their use beyond well equipped laboratories. In the meantime, microscopy in resource-limited settings has requirements significantly different from those encountered in advanced laboratories, and such imaging devices should be cost-effective, compact, light-weight and appropriately accurate and simple to be usable by minimally trained personnel. Furthermore, these portable microscopes should ideally be digitally integrated as part of a telemedicine network that connects various mobile health-care providers to a central laboratory or hospital. Toward this end, here we demonstrate a lensless on-chip microscope weighing approximately 46 grams with dimensions smaller than 4.2 cm x 4.2 cm x 5.8 cm that achieves sub-cellular resolution over a large field of view of approximately 24 mm(2). This compact and light-weight microscope is based on digital in-line holography and does not need any lenses, bulky optical/mechanical components or coherent sources such as lasers. Instead, it utilizes a simple light-emitting-diode (LED) and a compact opto-electronic sensor-array to record lensless holograms of the objects, which then permits rapid digital reconstruction of regular transmission or differential interference contrast (DIC) images of the objects. Because this lensless incoherent holographic microscope has orders-of-magnitude improved light collection efficiency and is very robust to mechanical misalignments it may offer a cost-effective tool especially for telemedicine applications involving various global health problems in resource limited settings.

  19. Compact, Light-weight and Cost-effective Microscope based on Lensless Incoherent Holography for Telemedicine Applications

    PubMed Central

    Mudanyali, Onur; Tseng, Derek; Oh, Chulwoo; Isikman, Serhan O.; Sencan, Ikbal; Bishara, Waheb; Oztoprak, Cetin; Seo, Sungkyu; Khademhosseini, Bahar; Ozcan, Aydogan

    2010-01-01

    Despite the rapid progress in optical imaging, most of the advanced microscopy modalities still require complex and costly set-ups that unfortunately limit their use beyond well equipped laboratories. In the meantime, microscopy in resource-limited settings has requirements significantly different from those encountered in advanced laboratories, and such imaging devices should be cost-effective, compact, light-weight and appropriately accurate and simple to be usable by minimally trained personnel. Furthermore, these portable microscopes should ideally be digitally integrated as part of a telemedicine network that connects various mobile health-care providers to a central laboratory or hospital. Toward this end, here we demonstrate a lensless on-chip microscope weighing ~46 grams with dimensions smaller than 4.2cm × 4.2cm × 5.8cm that achieves sub-cellular resolution over a large field of view of ~24 mm2. This compact and light-weight microscope is based on digital in-line holography and does not need any lenses, bulky optical/mechanical components or coherent sources such as lasers. Instead, it utilizes a simple light-emitting-diode (LED) and a compact opto-electronic sensor-array to record lensless holograms of the objects, which then permits rapid digital reconstruction of regular transmission or differential interference contrast (DIC) images of the objects. Because this lensless incoherent holographic microscope has orders-of-magnitude improved light collection efficiency and is very robust to mechanical misalignments it may offer a cost-effective tool especially for telemedicine applications involving various global health problems in resource limited settings. PMID:20401422

  20. Digital microscopy using phase-shifting digital holography with two reference waves.

    PubMed

    Guo, Pengyi; Devaney, Anthony J

    2004-04-15

    A lensless, coherent optical microscope is described that uses a version of phase-shifting digital holography (PSDH) in conjunction with a field backpropagation algorithm to form coherent images of transmission-type objects. The PSDH is implemented by use of only two reference waves, in contrast with the usual implementation that requires four quadrature phase-shifting reference waves. Therefore only two digital holograms need to be recorded, and the complexity of the microscopic system is reduced. Experimental results are presented that compare images generated from conventional Gabor digital holography, two-reference-wave PSDH, and conventional white-light microscopy.

  1. Generalized phase-shifting color digital holography

    NASA Astrophysics Data System (ADS)

    Nomura, Takanori; Kawakami, Takaaki; Shinomura, Kazuma

    2016-06-01

    Two methods to apply the generalized phase-shifting digital holography to color digital holography are proposed. One is wave-splitting generalized phase-shifting color digital holography. This is realized by using a color Bayer camera. Another is multiple exposure generalized phase-shifting color digital holography. This is realized by the wavelength-dependent phase-shifting devices. Experimental results for both generalized phase-shifting color digital holography are presented to confirm the proposed methods.

  2. Lensless coherent imaging by sampling of the optical field with digital micromirror device

    NASA Astrophysics Data System (ADS)

    Vdovin, G.; Gong, H.; Soloviev, O.; Pozzi, P.; Verhaegen, M.

    2015-12-01

    We have experimentally demonstrated a lensless coherent microscope based on direct registration of the complex optical field by sampling the pupil with a sequence of two-point interferometers formed by a digital micromirror device. Complete registration of the complex amplitude in the pupil of the imaging system, without any reference beam, provides a convenient link between the experimental and computational optics. Unlike other approaches to digital holography, our method does not require any external reference beam, resulting in a simple and robust registration setup. Computer analysis of the experimentally registered field allows for focusing the image in the whole range from zero to infinity, and for virtual correction of the aberrations present in the real optical system, by applying the adaptive wavefront corrections to its virtual model.

  3. Spatially incoherent Fourier digital holography

    NASA Astrophysics Data System (ADS)

    Nomura, Takanori; Watanabe, Kaho

    2017-05-01

    The possibility of incoherent digital holography has been widely studied because it is free from coherent light sources. Here spatially incoherent Fourier digital holography is described. The incoherent hologram is obtained by a rotational shearing interferometer. The hologram obtained by the interferometer is a cosine transform of a spatially incoherent object. After describing the principle of a rotational shearing interferometer, methods to obtain Fourier transform of an object presented.

  4. Color lensless digital holographic microscopy with micrometer resolution.

    PubMed

    Garcia-Sucerquia, Jorge

    2012-05-15

    Color digital lensless holographic microscopy with micrometer resolution is presented. Multiwavelength illumination of a biological sample and a posteriori color composition of the amplitude images individually reconstructed are used to obtain full-color representation of the microscopic specimen. To match the sizes of the reconstructed holograms for each wavelength, a reconstruction algorithm that allows for choosing the pixel size at the reconstruction plane independently of the wavelength and the reconstruction distance is used. The method is illustrated with experimental results.

  5. Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization.

    PubMed

    Bourgade, Thomas; Jianfei, Sun; Wang, Zhaomin; Elsa, Rosmin; Asundi, Anand

    2016-07-05

    A micro-electro-mechanical-system (MEMS) is a widely used component in many industries, including energy, biotechnology, medical, communications, and automotive. However, effective inspection and characterization metrology systems are needed to ensure the functional reliability of MEMS. This study presents a system based on digital holography as a tool for MEMS metrology. Digital holography has gained increasing attention in the past 20 years. With the fast development and decreasing cost of sensor arrays, resolution of such systems has increased broadening potential applications. Thus, it has attracted attention from both research and industry sides as a potential reliable tool for industrial metrology. Indeed, by recording the interference pattern between an object beam (which contains sample height information) and a reference beam on a CCD camera, one can retrieve the quantitative phase information of an object. However, most of digital holographic systems are bulky and thus not easy to implement on industry production lines. The novelty of the system presented is that it is lens-less and thus very compact. In this study, it is shown that the Compact Digital Holographic Microscope (CDHM) can be used to evaluate several characteristics typically consider as criteria in MEMS inspections. The surface profiles of MEMS in both static and dynamic conditions are presented. Comparison with AFM is investigated to validate the accuracy of the CDHM.

  6. Short-coherence digital microscopy by use of a lensless holographic imaging system.

    PubMed

    Pedrini, Giancarlo; Tiziani, Hans J

    2002-08-01

    An optical system based on short-coherence digital holography suitable for three-dimensional (3D) microscopic investigations is described. The light source is a short-coherence laser, and the holograms are recorded on a CCD sensor. The interference (hologram) occurs only when the path lengths of the reference and the object beam are matched within the coherence length of the laser. The image of the part of the sample that matches the reference beam is reconstructed by numerical evaluation of the hologram. The advantages of the method are high numerical aperture (this means high spatial resolution), detection of the 3D shape, and a lensless imaging system. Experimental results are presented.

  7. Digital Holography Display (2)

    NASA Astrophysics Data System (ADS)

    Lee, Cheok Peng; Asundi, A.; Yu, Yang; Xiao, Zhen Zhong

    This paper describes the extension work from the last Digital Holography Projector System. From the developed works shows that, some unforeseen factors have created the difficulties for the system alignment. Such factors are the DMD frame rate, light source and diffractive zero order. It is really the challenging development works to achieve the virtual 3D model display on the high speed rotation screen. The three most key factors are emphasizing: 1) The display device's frame rate; 2) The light source orientation angle; and 3) The zero order filtering optic. 1) This device's is the digital micro mirror, in short is DMD. It is the high speed switching device has developed by the most recent technology. The switching frame rate can go up as high as 291fps. At first, the 8 bits depth file must be digitalized and stored for DMD onboard Ram. The digitalized data are transmitting from the PC USB to DMD onboard Ram. Instead of the data are downloading directly from the PC to DVI or VGA during display, this downloading method cause slower down the display speed, which is the common frame rate of 30 Hz. Next, the onboard Ram data then transfer to the DMD mirror's for display, at the 8 bits 291 fps speed. At this frame rate, the display 2D image can almost cover for 10 of out of the 360 0 in 1 revolution. 2) This laser light source must be installed such that free for orientated in any arbitrary angle from 220 to 450. Which is normalized to the DMD mirrors and the brief sketch show on figure (a). The purpose of orientated the light source is ensure that multi diffractive order would be reflected straight from the mirrors. (This multi diffractive order is the phenomenon of the digital micro mirror's characteristic). This mean, the reconstruct images would be followed the DMD normalized direction reflected up to fibre conduit. Moreover, this orientated method install of the laser light source is making space for other optical lenses or device driver/controller. Because, all

  8. Femtosecond digital lensless holographic microscopy to image biological samples.

    PubMed

    Mendoza-Yero, Omel; Calabuig, Alejandro; Tajahuerce, Enrique; Lancis, Jesús; Andrés, Pedro; Garcia-Sucerquia, Jorge

    2013-09-01

    The use of femtosecond laser radiation in digital lensless holographic microscopy (DLHM) to image biological samples is presented. A mode-locked Ti:Sa laser that emits ultrashort pulses of 12 fs intensity FWHM, with 800 nm mean wavelength, at 75 MHz repetition rate is used as a light source. For comparison purposes, the light from a light-emitting diode is also used. A section of the head of a drosophila melanogaster fly is studied with both light sources. The experimental results show very different effects of the pinhole size on the spatial resolution with DLHM. Unaware phenomena on the field of the DLHM are analyzed.

  9. Diffractive digital lensless holographic microscopy with fine spectral tuning.

    PubMed

    Mendoza-Yero, Omel; Tajahuerce, Enrique; Lancis, Jesús; Garcia-Sucerquia, Jorge

    2013-06-15

    We experimentally demonstrate an all-diffractive optical setup for digital lensless holographic microscopy with easy wavelength line selection and micrometric resolution. In the proposed system, an ultrashort laser pulse is focused with a diffractive lens (DL) onto a pinhole of diameter close to its central wavelength to achieve a highly spatially coherent illumination cone as well as a spectral line with narrow width. To scan the complete spectrum of the light source the DL is displaced with respect to the pinhole plane. The proposed microscopy setup allows us to spectrally separate contributions from different sections of a sample, which may be attractive for several applications in life sciences.

  10. Lensless Digital Holographic Microscopy for Life Detection

    NASA Astrophysics Data System (ADS)

    Serabyn, E.; Liewer, K.; Wallace, J. K.; Rider, S.; Lindensmith, C.; Nadeau, J.

    2016-10-01

    Microscopy capable of volume imaging can be used to search for microbial life on ocean worlds. Here we discuss our recent digital holographic microscope (DHM) systems, which provide micron-scale resolution in a very compact package.

  11. Color digital lensless holographic microscopy: laser versus LED illumination.

    PubMed

    Garcia-Sucerquia, Jorge

    2016-08-20

    A comparison of the performance of color digital lensless holographic microscopy (CDLHM) as utilized for illumination of RGB lasers or a super-bright white-light LED with a set of spectral filters is presented. As the use of lasers in CDLHM conceals the possibility of having a compact, lightweight, portable, and low cost microscope, and additionally the limited available laser radiation wavelengths limit a real multispectral imaging microscope, here we present the use of super-bright white-light LED and spectral filters for illuminating the sample. The performance of RGB laser-CDLHM and LED-CDLHM is evaluated on imaging a section of the head of a Drosophila melanogaster fly. This comparison shows that there is trade-off between the spatial resolution of the microscope and the light sources utilized, which can be understood with regard to the coherence properties of the illuminating light. Despite the smaller spatial coherence features of LED-CDLHM in comparison with laser-CDLHM, the former shows promise as a portable RGB digital lensless holographic microscope that could be extended to other wavelengths by the use of different spectral filters.

  12. Phase-shifting digital holography.

    PubMed

    Yamaguchi, I; Zhang, T

    1997-08-15

    A new method for three-dimensional image formation is proposed in which the distribution of complex amplitude at a plane is measured by phase-shifting interferometry and then Fresnel transformed by a digital computer. The method can reconstruct an arbitrary cross section of a three-dimensional object with higher image quality and a wider viewing angle than from conventional digital holography using an off-axis configuration. Basic principles and experimental verification are described.

  13. Digital holography and 3-D imaging.

    PubMed

    Banerjee, Partha; Barbastathis, George; Kim, Myung; Kukhtarev, Nickolai

    2011-03-01

    This feature issue on Digital Holography and 3-D Imaging comprises 15 papers on digital holographic techniques and applications, computer-generated holography and encryption techniques, and 3-D display. It is hoped that future work in the area leads to innovative applications of digital holography and 3-D imaging to biology and sensing, and to the development of novel nonlinear dynamic digital holographic techniques.

  14. Temperature measurement in laminar free convective flow using digital holography.

    PubMed

    Hossain, Md Mosarraf; Shakher, Chandra

    2009-04-01

    A method for measurement of temperature in laminar free convection flow of water is presented using digital holographic interferometry. The method is relatively simple and fast because the method uses lensless Fourier transform digital holography, for which the reconstruction algorithm is simple and fast, and also the method does not require use of any extra experimental efforts as in phase shifting. The quantitative unwrapped phase difference is calculated experimentally from two digital holograms recorded in two different states of water--one in the quiescent state, the other in the laminar free convection. Unknown temperature in laminar free convection is measured quantitatively using a known value of temperature in the quiescent state from the unwrapped phase difference, where the equation by Tilton and Taylor describing the variation of refractive index of water with temperature is used to connect the phase with temperature. Experiments are also performed to visualize the turbulent free convection flow.

  15. Imaging live humans through smoke and flames using far-infrared digital holography.

    PubMed

    Locatelli, M; Pugliese, E; Paturzo, M; Bianco, V; Finizio, A; Pelagotti, A; Poggi, P; Miccio, L; Meucci, R; Ferraro, P

    2013-03-11

    The ability to see behind flames is a key challenge for the industrial field and particularly for the safety field. Development of new technologies to detect live people through smoke and flames in fire scenes is an extremely desirable goal since it can save human lives. The latest technologies, including equipment adopted by fire departments, use infrared bolometers for infrared digital cameras that allow users to see through smoke. However, such detectors are blinded by flame-emitted radiation. Here we show a completely different approach that makes use of lensless digital holography technology in the infrared range for successful imaging through smoke and flames. Notably, we demonstrate that digital holography with a cw laser allows the recording of dynamic human-size targets. In this work, easy detection of live, moving people is achieved through both smoke and flames, thus demonstrating the capability of digital holography at 10.6 μm.

  16. Twin image removal in digital in-line holography based on iterative inter-projections

    NASA Astrophysics Data System (ADS)

    Chen, Bing Kuan; Chen, Tai-Yu; Hung, Shau Gang; Huang, Sheng-Lung; Lin, Jiunn-Yuan

    2016-06-01

    A simple and efficient phase retrieval method based on the iterative inter-projections of the recorded Fourier modulus between two effective holographic planes is developed to eliminate the twin image in digital in-line holography. The proposed algorithm converges stably in phase extraction procedures without requiring any prior knowledge or sophisticated support of the object and is applicable to lensless Gabor and Fourier holography as well as holographic microscopy with imaging lenses. Numerical and experimental results suggest that the spatial resolution enhancement on the reconstructed image can be achieved with this technique due to the capability of recovering the diffraction phases of low-intensity signals.

  17. Multiwavelength digital holography with autocalibration of phase shifts and artificial wavelengths

    SciTech Connect

    Carl, Daniel; Fratz, Markus; Pfeifer, Marcel; Giel, Dominik M.; Hoefler, Heinrich

    2009-12-01

    A novel implementation of lensless multiwavelength digital holography with autocalibration of temporal phase shifts and artificial wavelength is presented. The algorithm we used to calculate the phase shifts was previously proposed [Opt. Lett.29 183 (2004)OPLEDP0146-959210.1364/OL.29.000183] and, to our knowledge, is now used for the first time in lensless holography. Because precise knowledge of the generated artificial wavelength is crucial for absolute measurement accuracy, a simple and efficient method to determine the artificial wavelength directly is presented. The calibration method is based on a simple modification of the experimental setup and needs just one additional image acquisition per wavelength. The results of shape measurement of a metallic test object with a rough surface and steep edges are shown and the measurement accuracy is discussed.

  18. Lensless Imaging and Sensing.

    PubMed

    Ozcan, Aydogan; McLeod, Euan

    2016-07-11

    High-resolution optical microscopy has traditionally relied on high-magnification and high-numerical aperture objective lenses. In contrast, lensless microscopy can provide high-resolution images without the use of any focusing lenses, offering the advantages of a large field of view, high resolution, cost-effectiveness, portability, and depth-resolved three-dimensional (3D) imaging. Here we review various approaches to lensless imaging, as well as its applications in biosensing, diagnostics, and cytometry. These approaches include shadow imaging, fluorescence, holography, superresolution 3D imaging, iterative phase recovery, and color imaging. These approaches share a reliance on computational techniques, which are typically necessary to reconstruct meaningful images from the raw data captured by digital image sensors. When these approaches are combined with physical innovations in sample preparation and fabrication, lensless imaging can be used to image and sense cells, viruses, nanoparticles, and biomolecules. We conclude by discussing several ways in which lensless imaging and sensing might develop in the near future.

  19. Digital holography based submicron thermometry.

    PubMed

    Moradi, Amin; Akhlaghi, Ehsan A; Hajizedeh, Faegheh; Reihani, S Nader S

    2016-12-12

    Here we introduce a phase-shifting digital holography-based method to determine the temperature profile around an irradiated (sub-)micron spherical bead. The method utilizes a Mach-Zehnder interferometer implemented into an open setup microscope. The results of irradiated gold spheres with diameter of 400 nm and also silver-coated micron-sized silica beads embedded in silicone oil are presented. We show that the applied method is able to accurately determine the surface temperature with accuracy of 1 °C. Our experimental results perfectly confirm the theoretical prediction of temperature profile around the irradiated bead.

  20. DYI digital holography

    NASA Astrophysics Data System (ADS)

    Zacharovas, Stanislovas; Nikolskij, Andrej; Kuchin, Jevgenij

    2011-02-01

    We have created a programming tool which uses image data provided by webcam connected to personal computer and gives user an ability to see the future digital hologram preview on his computer screen, before sending video data to holographic printing companies. In order to print digital hologram, one needs to have a sequence of images of the same scene taken from different angles and nowadays web cameras - stand-alone or incorporated into mobile computer, can be an acceptable source of such image sequences. In this article we are describing this DIY holographic imaging process in details.

  1. Direct to Digital Holography

    SciTech Connect

    Bingham, P.R.; Tobin, K.W.

    2003-06-15

    In this CRADA, Oak Ridge National Laboratory (ORNL) assisted nLine Corporation of Austin, TX in the development of prototype semiconductor wafer inspection tools based on the direct-to-digital holographic (DDH) techniques invented at ORNL. Key components of this work included, development of the first prototype named the Visible Alpha Tool (VAT) that uses visible spectrum illumination of 532 nm, assist in design of second prototype tool named the DUV Alpha Tool (DAT) using deep UV (266 nm) illumination, and continuing support of nLine in the development of higher throughput commercial tools.

  2. Direct to Digital Holography

    SciTech Connect

    Bingham, P.R.; Tobin, K.W.

    2002-06-15

    In this CRADA, Oak Ridge National Laboratory (ORNL) assisted nLine Corporation of Austin, TX in the development of prototype semiconductor wafer inspection tools based on the direct-to-digital holographic (DDH) techniques invented at ORNL. Key components of this work included, testing of DDH for detection of defects in High Aspect Ratio (HAR) structures, development of image processing techniques to enhance detection capabilities through the use of both phase and intensity, and development of methods for autofocus on the DDH tools.

  3. Direct to Digital Holography

    SciTech Connect

    Bingham, P.R.; Tobin, K.W.

    2007-09-30

    In this Cooperative Research and Development Agreement (CRADA), Oak Ridge National Laboratory (ORNL) assisted nLine Corporation of Austin, TX in the development of prototype semiconductor wafer inspection tools based on the direct-to-digital holographic (DDH) techniques invented at ORNL. Key components of this work included, testing of DDH for detection of defects in High Aspect Ratio (HAR) structures, development of image processing techniques to enhance detection capabilities through the use of both phase and intensity, and development of methods for autofocus on the DDH tools.

  4. Visualizing Breath using Digital Holography

    NASA Astrophysics Data System (ADS)

    Hobson, P. R.; Reid, I. D.; Wilton, J. B.

    2013-02-01

    Artist Jayne Wilton and physicists Peter Hobson and Ivan Reid of Brunel University are collaborating at Brunel University on a project which aims to use a range of techniques to make visible the normally invisible dynamics of the breath and the verbal and non-verbal communication it facilitates. The breath is a source of a wide range of chemical, auditory and physical exchanges with the direct environment. Digital Holography is being investigated to enable a visually stimulating articulation of the physical trajectory of the breath as it leaves the mouth. Initial findings of this research are presented. Real time digital hologram replay allows the audience to move through holographs of breath-born particles.

  5. Label-free coherent microscopy through blood by digital holography

    NASA Astrophysics Data System (ADS)

    Bianco, V.; Paturzo, M.; Merola, F.; Miccio, L.; Memmolo, P.; Gennari, O.; Ferraro, P.

    2015-05-01

    Digital Holography (DH) numerical methods have been developed to allow imaging through turbid media. DH is a wide used imaging technique as it provides non-invasive quantitative phase-contrast mapping as well as flexible numerical refocusing of samples acquired in lens-based or lensless conditions. However, a challenging issue has to be faced when the samples are immersed inside a dynamic turbid medium, as biological occluding objects out of interest provoke severe light scattering or unpredictable time-variable phase delays which scramble the object information, so that in many cases the sample is not visible at all. Here we show a simple technique, named Multi-Look Digital Holography (MLDH), able to fully recover the useful signal of the specimen of interest dipped inside the turbid liquid phase. Multiple hologram recordings are incoherently combined to synthesize the whole complex field carrying the useful information, thus revealing the hidden objects. In particular, it will be shown that both amplitude imaging and phase-contrast mapping of cells hidden behind a flow of Red Blood Cells can be obtained. Besides, qualitative comparison and quantitative evaluation show a remarkable improvement with respect to the image captured when the cells were immersed in a transparent medium. In other words, the RBCs have been demonstrated to accomplish an optical task, acting as a speckle noise de-correlation device.

  6. Multi-imaging capabilities of a 2D diffraction grating in combination with digital holography.

    PubMed

    Paturzo, Melania; Merola, Francesco; Ferraro, Pietro

    2010-04-01

    In this Letter we report on an alternative approach to get multiple images in microscopy, exploiting the capabilities of both a lithium niobate diffraction grating and digital holographic technique. We demonstrate that multi-imaging can be achieved in a lensless configuration by using a hexagonal diffraction grating but overcoming, thanks to digital holography (DH), the many constrains imposed by the grating parameters in multi-imaging with Talbot effect or Talbot array illuminators. In fact, DH permits the numerical reconstruction of the optical field diffracted by the grating, thus obtaining in-focus multiple images in a plane different from the fractional or entire Talbot ones.

  7. Digital holography-based steganography.

    PubMed

    Hamam, Habib

    2010-12-15

    A steganographic method offering a high hiding capacity is presented in which the techniques of digital holography are used to distribute information from a small secret image across the larger pixel field of a cover image. An iterative algorithm is used to design a phase-only or complex hologram from a padded version of the secret image, quantizing this data according to the carrier data bits that are available within the intended cover image. By introducing the hologram data only into low-order bits of larger amplitude cover pixels, the change in the cover image remains imperceptible to the casual observer, with a peak signal-to-noise ratio of >40 dB.

  8. Depth-filtered digital holography.

    PubMed

    Koukourakis, Nektarios; Jaedicke, Volker; Adinda-Ougba, Adamou; Goebel, Sebastian; Wiethoff, Helge; Höpfner, Henning; Gerhardt, Nils C; Hofmann, Martin R

    2012-09-24

    We introduce depth-filtered digital holography (DFDH) as a method for quantitative tomographic phase imaging of buried layers in multilayer samples. The procedure is based on the acquisition of multiple holograms for different wavelengths. Analyzing the intensity over wavelength pixel wise and using an inverse Fourier transform leads to a depth-profile of the multilayered sample. Applying a windowed Fourier transform with a narrow window, we choose a depth-of interest (DOI) which is used to synthesize filtered interference patterns that just contain information of this limited depth. We use the angular spectrum method to introduce an additional spatial filtering and to reconstruct the corresponding holograms. After a short theoretical framework we show experimental proof-of-principle results for the method.

  9. Microstructure measurement of digital holography

    NASA Astrophysics Data System (ADS)

    Li, Jicheng; Tian, Ailing; Wang, Hongjun; Zhu, Xueliang; Wang, Chunhui; Liu, Bingcai; Asundi, Anand

    2015-02-01

    Miniaturization is a development trend of electronics, machinery and information systems, while micro structure brought a large amount of new development for industrial and research applications, whereas, slow measuring speed and two-dimensional results of traditional micro measurement could not meet our needs, it's urgent to find a matching testing techniques with more sensitivity, more effectiveness and better to have a real-time three-dimensional display. Digital holography applied to the measurement of micro structure, it made up for the lacking of traditional micro structure measure systems of too much time consuming, poor immunity, easily damaging samples with its simple structure, high accuracy, non contact features and three-dimensional reproduction. This paper analyzed the key factors of digital holographic recording and reproducing process.In order to solve the low quality of holograms captured by traditional recording system, holograms and pre-processing algorithm was combined for real-time, by observing the holograms and delicate adjusting the system, to ensure that the collected holograms with full use of CCD width while convenient for subsequent processing. In the processing of reproduction,the influence of spectrum choice, reconstruction wavelength and algorithms and unwrapping algorithm was been studied, and finally obtained an accurate three-dimensional topography of the object. The improved rerecording system and reconstruction algorithm mentioned above solved the low holography quality, much noise and not clear shortcomings of the reconstructed image. Experiment on a raster, compared with traditional system and algorithm results, results showed that the recording system and determine algorithms can reproduce the three-dimensional topography of the object with high precision and has a broader applicability.

  10. Digital holography to light field

    NASA Astrophysics Data System (ADS)

    Asundi, Anand; Zuo, Chao

    2014-05-01

    Holography uses wave (physical) optical principles of interference and diffraction to record and display images. Interference allows us to record the amplitude and phase of the optical wave emanating from an object on a film or recording medium and diffraction enables us to see this wave-field, i.e. the amplitude and phase of the object. Visually this corresponds to both perspective and depth information being reconstructed as in the original scene. Digital Holography has enabled quantification of phase which in some applications provides meaningful engineering parameters. There is growing interest in reconstructing this wavefield without interference. Thus the non-interferometric Transport of Intensity Equation (TIE) method is gaining increased research, which uses two or more defocused images to reconstruct the phase. Due to its non-interferometric nature, TIE relaxes the stringent beam-coherence requirements for interferometry, extending its applications to various optical fields with arbitrary spatial and temporal coherence. The alternate school of thought emerges from the computer science community primarily deals with ray optics. In a normal imaging system all rays emerging from an object point into are focused to a conjugate image point. Information of ray direction is lost and thus the perspective and depth information. A light field image is one that has information of both amplitude and direction of rays fanning from any object point and thus provides perspective (or what could be termed as phase) of the object wave as well. It would thus be possible to extract phase as we know it from this albeit for a coherent illumination case.

  11. Free-viewpoint images captured using phase-shifting synthetic aperture digital holography.

    PubMed

    Nakatsuji, Tatsuya; Matsushima, Kyoji

    2008-07-01

    Free-viewpoint images obtained from phase-shifting synthetic aperture digital holography are given for scenes that include multiple objects and a concave object. The synthetic aperture technique is used to enlarge the effective sensor size and to make it possible to widen the range of changing perspective in the numerical reconstruction. The lensless Fourier setup and its aliasing-free zone are used to avoid aliasing errors arising at the sensor edge and to overcome a common problem in digital holography, namely, a narrow field of view. A change of viewpoint is realized by a double numerical propagation and by clipping the wave field by a given pupil. The computational complexity for calculating an image in the given perspective from the base complex-valued image is estimated at a double fast Fourier transform. The experimental results illustrate the natural change of appearance in cases of both multiple objects and a concave object.

  12. High resolution digital holography based on the point source scanning

    NASA Astrophysics Data System (ADS)

    Wang, Minchao; Wang, Dayong; Rong, Lu; Wang, Yunxin; Wang, Fengpeng; Lin, Qiaowen

    2016-10-01

    Digital holographic microscopy has been widely used for the imaging of micro-objects and biological samples. Lensless in-line digital holographic microscopy is capable of wide field-of-view imaging. However the spatial resolution of the reconstructed images is limited by the pixel size of the detector. The relative position shift between the sample and the detector can effectively improve the resolution in the traditional sub-pixel shifting method, but it requires a high precision of translation stage. To overcome this problem, we propose a method based on the point source scanning to realize sub-pixel shifting. High precision sub-pixel shifting is achieved easily by using the geometric between point source and detector. Through moving the point source, multiple holograms with sub-pixel shifts are captured. These holograms are merged together to obtained a high resolution hologram by a synthesizing algorithm. Then, the high resolution reconstructed image of the object can be obtained by the angular spectrum algorithm. The feasibility of the proposed method is demonstrated by simulation and experiments. A USAF resolution test target was used as the object. Compared with the traditional digital holography, a higher resolution reconstructed image is obtained by our method. The proposed method has the advantages of simple recording setup and lower precision requirement of the translation stage. It can achieve the wide field-of-view and high resolution imaging.

  13. Fiber-based lensless polarization holography for measuring Jones matrix parameters of polarization-sensitive materials.

    PubMed

    Liu, Xuan; Yang, Yang; Han, Lu; Guo, Cheng-Shan

    2017-04-03

    We report a fiber-based lensless holographic imaging system to realize a single-shot measurement of two dimensional (2-D) Jones matrix parameters of polarization-sensitive materials. In this system, a multi-source lensless off-axis Fresnel holographic recording geometry is adopted, and two optical fiber splitters are used to generate the multiple reference and illumination beams required for recording a four-channel angular-multiplexing polarization hologram (AMPH). Using this system and the method described in this paper, spatially resolved Jones matrix parameters of a polarization-sensitive material can be retrieved from one single-shot AMPH. We demonstrate the feasibility of the method by extracting a 2-D Jones matrix of a composite polarizer. Applications of the method to measure the Jones matrix maps of a stressed polymethyl methacrylate sample and a mica fragment are also presented. Benefit from the fiber-based and lensless off-axis holographic design, the system possesses a quite compact configuration, which provides a feasible approach for development of an integrated and portable system to measure Jones matrix parameters of polarization-sensitive materials.

  14. Compact lensless off-axis transmission digital holographic microscope.

    PubMed

    Rostykus, Manon; Moser, Christophe

    2017-07-10

    Current compact lensless holographic microscopes are based on either multiple angle in-line holograms, multiple wavelength illumination or a combination thereof. Complex computational algorithms are necessary to retrieve the phase image which slows down the visualization of the image. Here we propose a simple compact lensless transmission holographic microscope with an off-axis configuration which simplifies considerably the computational processing to visualize the phase images and opens the possibility of real time phase imaging using off the shelf smart phone processors and less than $3 worth of optics and detectors, suitable for broad educational dissemination. This is achieved using a side illumination and analog hologram gratings to shape the reference and signal illumination beams from one light source. We demonstrate experimentally imaging of cells with a field of view (FOV) of ~12mm(2), and a resolution of ~3.9μm.

  15. Digital holography with ultimate sensitivity.

    PubMed

    Gross, M; Atlan, M

    2007-04-15

    We propose a variant of the heterodyne holography scheme that combines the properties of off-axis and phase-shifting holography. This scheme makes it possible to filter off numerically the zero-order image alias and the technical noise of the reference. It is then possible to record and reconstruct holographic images at an extremely low signal level. We show experimentally that the sensitivity of the method is limited only by the quantum nature of photons.

  16. Refocusing criterion via sparsity measurements in digital holography.

    PubMed

    Memmolo, Pasquale; Paturzo, Melania; Javidi, Bahram; Netti, Paolo A; Ferraro, Pietro

    2014-08-15

    Several automatic approaches have been proposed in the past to compute the refocus distance in digital holography (DH). However most of them are based on a maximization or minimization of a suitable amplitude image contrast measure, regarded as a function of the reconstruction distance parameter. Here we show that, by using the sparsity measure coefficient regarded as a refocusing criterion in the holographic reconstruction, it is possible to recover the focus plane and, at the same time, establish the degree of sparsity of digital holograms, when samples of the diffraction Fresnel propagation integral are used as a sparse signal representation. We employ a sparsity measurement coefficient known as Gini's index thus showing for the first time, to the best of our knowledge, its application in DH, as an effective refocusing criterion. Demonstration is provided for different holographic configurations (i.e., lens and lensless apparatus) and for completely different objects (i.e., a thin pure phase microscopic object as an in vitro cell, and macroscopic puppets) preparation.

  17. Digital holography of total internal reflection.

    PubMed

    Ash, William M; Kim, Myung K

    2008-06-23

    We introduce a new microscopy technique termed total internal reflection holographic microscopy (TIRHM). Quantitative phase microscopy by digital holography is used to image the phase profile of light in total internal reflection, which is modulated by the materials present on or near the surface of internal reflection. The imaging characteristics are theoretically modeled and imaging capabilities are experimentally demonstrated.

  18. Multispectral digital lensless holographic microscopy: from femtosecond laser to white light LED

    NASA Astrophysics Data System (ADS)

    Garcia-Sucerquia, J.

    2015-04-01

    The use of femtosecond laser radiation and super bright white LED in digital lensless holographic microscopy is presented. For the ultrafast laser radiation two different configurations of operation of the microscope are presented and the dissimilar performance of each one analyzed. The microscope operating with a super bright white light LED in combination with optical filters shows very competitive performance as it is compared with more expensive optical sources. The broadband emission of both radiation sources allows the multispectral imaging of biological samples to obtain spectral responses and/or full color images of the microscopic specimens; sections of the head of a Drosophila melanogaster fly are imaged in this contribution. The simple, solid, compact, lightweight, and reliable architecture of digital lensless holographic microscopy operating with broadband light sources to image biological specimens exhibiting micrometer-sized details is evaluated in the present contribution.

  19. A compact and lensless digital holographic microscope setup

    NASA Astrophysics Data System (ADS)

    Hu, Yan; Zuo, Chao; Sun, Jiasong; Chen, Qian; Zhang, Yuzhen

    2015-07-01

    We design a holographic system which is lensless and compact. There is a beam expander in conventional holographic setup to produce parallel light and then with a beam splitter to separate the light into two parts. One is used to illuminate the objects and the other one as the reference light. In our system, instead of utilizing beam expander to generalize parallel beam, the laser is directly produced by a fiber, which provides a spherical wave with a center in the out port of fiber. For this reason, our system contains less optical components so that the setup would be more compact. The only needed processing is to eliminate the second-order aberration caused by different distance between two path and the off-axis to a small extent. An experiment of aberration compensation by using principle component analysis is given, and the result shows that the system works well.

  20. Two-dimensional Phase Unwrapping for Digital Holography

    DTIC Science & Technology

    2012-09-01

    Two-dimensional Phase Unwrapping for Digital Holography by Neal K. Bambha, Justin R. Bickford, and Karl K. Klett, Jr. ARL-TR-6225...1197 ARL-TR-6225 September 2012 Two-dimensional Phase Unwrapping for Digital Holography Neal K. Bambha, Justin R. Bickford, and Karl K...2. REPORT TYPE Final 3. DATES COVERED (From - To) 4. TITLE AND SUBTITLE Two-dimensional Phase Unwrapping for Digital Holography 5a. CONTRACT

  1. Optical vortex phase-shifting digital holography.

    PubMed

    Guo, Cheng-Shan; Cheng, Xin; Ren, Xiu-Yun; Ding, Jian-Ping; Wang, Hui-Tian

    2004-10-18

    We report a new optical vortex phase-shifting method for digital holography, in which an optical vortex mode is taken as the reference beam for holographic recording, and the required phase shifts are directly generated by rotating the vortex mode. In digital reconstruction, the complex amplitude of the object wave can be retrieved by use of the conventional phase shifting algorithm on condition that the digital illumination beam is replaced by an vortex beam with the same topological charge as the reference used. Both the theoretical analysis and experimental results demonstrate the feasibility of this approach.

  2. Parallel three-step phase-shifting digital holography.

    PubMed

    Awatsuji, Yasuhiro; Fujii, Atsushi; Kubota, Toshihiro; Matoba, Osamu

    2006-05-01

    We propose parallel three-step phase-shifting digital holography as a technique capable of noiseless instantaneous measurement of three-dimensional objects based on phase-shifting interferometry. The proposed digital holography carries out three-step phase shifting at the same time by using a phase-shifting array device located in the reference beam. The array device has a periodic three-step phase distribution, and its configuration is simplified compared with that required for conventional parallel phase-shifting digital holography. Therefore the optical system of the proposed parallel phase-shifting digital holography is more suitable for the realization of the proposed holography. We conduct both a numerical simulation and a preliminary experiment. The results of the simulation and experiment agree well with those of the conventional phase-shifting method and are superior to the results obtained by conventional digital holography by using the Fresnel transform alone. Thus the effectiveness of the proposed technique is verified.

  3. Digital holography techniques for optical interference

    NASA Astrophysics Data System (ADS)

    Perez-Garcia, Benjamin; Gossman, David; Hernandez-Aranda, Raul I.; Forbes, Andrew

    2016-09-01

    In this work we revisit Young's experiment and show how it can be done with digital holography. We study different properties of light and show that depending on how light interferes, fringe patterns in other observables arise. We explain this conceptually and demonstrate how this can be implemented experimentally. We aid the reader with a tutorial-like approach and provide the necessary tools to easily perform the experiments.

  4. Quantitative phase microscopy with asynchronous digital holography.

    PubMed

    Chalut, Kevin J; Brown, William J; Wax, Adam

    2007-03-19

    We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acousto-optic modulators to execute phase-shifting interferometry using two near-simultaneous interferograms. The method can be used to obtain quantitative phase images of dynamic biological samples on millisecond time scales. We present results on a standard sample, and on live cell samples.

  5. Quantitative phase microscopy with asynchronous digital holography

    PubMed Central

    Brown, William J.; Wax, Adam

    2009-01-01

    We demonstrate a new method of measuring quantitative phase in imaging of biological materials. This method, asynchronous digital holography, employs knowledge of a moving fringe created by acousto-optic modulators to execute phase-shifting interferometry using two near-simultaneous interferograms. The method can be used to obtain quantitative phase images of dynamic biological samples on millisecond time scales. We present results on a standard sample, and on live cell samples. PMID:19532543

  6. Digital holography from shadowgraphic phase estimates.

    PubMed

    Eilenberger, Falk; Minardi, Stefano; Pliakis, Dimitris; Pertsch, Thomas

    2012-02-15

    We show experimentally that the recently proposed iterative shadowgraphic method can be applied to in-line digital holography, provided a suitable regularization step is introduced. We show that the method correctly solves the "twin-image" problem using just two samples of the intensity field scattered by a phase object. Field retrieval accuracy is significantly improved when compared to a reconstruction obtained with the defocusing variation algorithms.

  7. Lensless two-photon imaging through a multicore fiber with coherence-gated digital phase conjugation

    NASA Astrophysics Data System (ADS)

    Conkey, Donald B.; Stasio, Nicolino; Morales-Delgado, Edgar E.; Romito, Marilisa; Moser, Christophe; Psaltis, Demetri

    2016-04-01

    We performed near-diffraction limited two-photon fluorescence (TPF) imaging through a lensless, multicore-fiber (MCF) endoscope utilizing digital phase conjugation. The phase conjugation technique is compatible with commercially available MCFs with high core density. We demonstrate focusing of ultrashort pulses through an MCF and show that the method allows for resolution that is not limited by the MCF core spacing. We constructed TPF images of fluorescent beads and cells by digital scanning of the phase-conjugated focus on the target object and collection of the emitted fluorescence through the MCF.

  8. Study of the modulation characterization of phase-only liquid crystal spatial light modulator by digital holography

    NASA Astrophysics Data System (ADS)

    Panezai, Spozmai; Wang, Dayong; Zhao, Jie; Wang, Yunxin

    2012-10-01

    The liquid crystal spatial light modulator is becoming a more and more important device due to its wide applications. During its characterization, the phase modulation is the most important one. In this paper, the modulation characterization of a PLUTO phase-only spatial light modulator based on liquid crystal on silicon (LCOS) is studied by applying the lensless Fourier transform digital holography. In the digital holography, the LCOS is used as a reflected sample which modulates the phase of the incident wavefront with respect to the addressed gray level and gives the information of entire active region. This result is then compared with the result obtained by using conventional Michelson Interferometric method and both results are in good accordance with each other. At last the same holographic set up is used for the imaging of phase grating as a reflected object which is addressed to the LCOS and the clear profilometry of the grating is achieved.

  9. Lensless imaging of magnetic nanostructures by X-ray spectro-holography.

    PubMed

    Eisebitt, S; Lüning, J; Schlotter, W F; Lörgen, M; Hellwig, O; Eberhardt, W; Stöhr, J

    2004-12-16

    Our knowledge of the structure of matter is largely based on X-ray diffraction studies of periodic structures and the successful transformation (inversion) of the diffraction patterns into real-space atomic maps. But the determination of non-periodic nanoscale structures by X-rays is much more difficult. Inversion of the measured diffuse X-ray intensity patterns suffers from the intrinsic loss of phase information, and direct imaging methods are limited in resolution by the available X-ray optics. Here we demonstrate a versatile technique for imaging nanostructures, based on the use of resonantly tuned soft X-rays for scattering contrast and the direct Fourier inversion of a holographically formed interference pattern. Our implementation places the sample behind a lithographically manufactured mask with a micrometre-sized sample aperture and a nanometre-sized hole that defines a reference beam. As an example, we have used the resonant X-ray magnetic circular dichroism effect to image the random magnetic domain structure in a Co/Pt multilayer film with a spatial resolution of 50 nm. Our technique, which is a form of Fourier transform holography, is transferable to a wide variety of specimens, appears scalable to diffraction-limited resolution, and is well suited for ultrafast single-shot imaging with coherent X-ray free-electron laser sources.

  10. Generalized phase-shifting digital holography for single-exposure

    NASA Astrophysics Data System (ADS)

    Nomura, Takanori; Imbe, Masatoshi

    2015-07-01

    Single exposure phase-shifting digital holography is mandatory for recording/analyzing dynamic phenomena. As one of the candidate for the single exposure phase-shifting digital holography is the wave-splitting phase-shifting digital holography. We have proposed generalized phase-shifting digital holography as one of the wave-splitting phase-shifting digital holography. In the digital holography, the phase-shift quantity, which corresponds to the difference between the phase quantities of the reference wave on the adjacent pixels is generalized different from ordinal wave-splitting methods. Owing to the generalized phase-shifting, we can use a random-phase wave as the reference wave. The proposed phase-shifting digital holography enables us to record a fully-complex field of a dynamic phenomena without dc and conjugate terms using a commercially available optical devices. The relation between the property of the reference wave and the quality of the reconstructed images are discussed. Experimental results are given to confirm the proposed phase-shifting digital holography.

  11. Automatic method for focusing biological specimens in digital lensless holographic microscopy.

    PubMed

    Trujillo, Carlos A; Garcia-Sucerquia, Jorge

    2014-05-01

    A self-focusing method applicable to digital lensless holographic microscopy is presented. The method searches for the global minimum of the area enclosing a given amount of energy in a region surrounding the object of interest. The proposed modified enclosed energy method has been tested on self-focusing experimental holograms of a paramecium specimen and a section of the head of a drosophila melanogaster fly. The presented self-focusing technique also has been contrasted with some of the already reported methods to seek the best focus image.

  12. Backscatter digital holography of microparticles.

    PubMed

    Subedi, Nava R; Fowler, Nicholas B; Berg, Matthew J

    2013-05-20

    This work investigates a method for digital holographic imaging of microparticles. Traditional digital holographic techniques use a particle's forward scattered light to form the hologram, whereas here we use the backscattered light. Images of a particle are then computationally reconstructed from the backscatter hologram, and several examples of such reconstructions are presented. A potential advantage of this technique is that the backscatter holograms may be more sensitive to particle-surface details.

  13. Incoherent digital holograms acquired by interferenceless coded aperture correlation holography system without refractive lenses.

    PubMed

    Kumar, Manoj; Vijayakumar, A; Rosen, Joseph

    2017-09-14

    We present a lensless, interferenceless incoherent digital holography technique based on the principle of coded aperture correlation holography. The acquired digital hologram by this technique contains a three-dimensional image of some observed scene. Light diffracted by a point object (pinhole) is modulated using a random-like coded phase mask (CPM) and the intensity pattern is recorded and composed as a point spread hologram (PSH). A library of PSHs is created using the same CPM by moving the pinhole to all possible axial locations. Intensity diffracted through the same CPM from an object placed within the axial limits of the PSH library is recorded by a digital camera. The recorded intensity this time is composed as the object hologram. The image of the object at any axial plane is reconstructed by cross-correlating the object hologram with the corresponding component of the PSH library. The reconstruction noise attached to the image is suppressed by various methods. The reconstruction results of multiplane and thick objects by this technique are compared with regular lens-based imaging.

  14. EDITORIAL: Optical tomography and digital holography

    NASA Astrophysics Data System (ADS)

    Coupland, Jeremy; Lobera, Julia

    2008-07-01

    The articles in this special feature in Measurement Science and Technology concern exciting new developments in the field of digital holography—the process of electronically recording and numerically reconstructing an optical field [1]. Making use of the enormous advances in digital imaging and computer technology, digital holography is presented in a range of applications from fluid flow measurement and structural analysis to medical imaging. The science of digital holography rests on the foundations of optical holography, on the work of Gabor in the late 1940s, and on the development of laser sources in the 1960s, which made his vision a practical reality [2]. Optical holography, however, uses a photosensitive material, both to record a latent image and subsequently to behave as a diffractive optical element with which to reconstruct the incident field. In this way display holograms, using silver halide materials for example, can produce life-size images that are virtually indistinguishable from the object itself [3]. Digital holography, in contrast, separates the steps of recording and reconstruction, and the final image is most often in the form of a 3D computer model. Of course, television cameras have been used from the beginnings of holography to record interferometric images. However, the huge disparity between the resolution of holographic recording materials (more than 3000 cycles/mm) and television cameras (around 50 cycles/mm) was raised as a major concern by early researchers. TV holography, as it was sometimes called, generally recorded low numerical aperture (NA) holograms producing images with characteristically large speckle and was therefore more often referred to as electronic speckle pattern interferomery (ESPI) [4]. It is possible, however, to record large NA holograms on a sensor with restricted resolution by using an objective lens or a diverging reference wave [5]. This is generally referred to as digital holographic microscopy (DHM) since

  15. Infrared digital holography for large objects investigation

    NASA Astrophysics Data System (ADS)

    Geltrude, A.; Locatelli, M.; Poggi, P.; Pelagotti, A.; Paturzo, M.; Ferraro, P.; Meucci, R.

    2011-05-01

    In this work we show several acquisition setups and techniques which make it possible to obtain holographic recording and reconstruction of large objects by means of Infrared Digital Holography (IDH). In previous works it was demonstrated that, using the long wavelength coherent radiation produced by a CO2 laser instead of visible radiation, it is possible to obtain advantages in terms of larger field of view and lower seismic noise sensitivity. The only drawback using this wavelength is represented by the low resolution of current recording devices in this spectral region. The reported methods may have industrial applications where investigation of large dimension samples is needed.

  16. Compressed digital holography: from micro towards macro

    NASA Astrophysics Data System (ADS)

    Schretter, Colas; Bettens, Stijn; Blinder, David; Pesquet-Popescu, Béatrice; Cagnazzo, Marco; Dufaux, Frédéric; Schelkens, Peter

    2016-09-01

    signal processing methods from software-driven computer engineering and applied mathematics. The compressed sensing theory in particular established a practical framework for reconstructing the scene content using few linear combinations of complex measurements and a sparse prior for regularizing the solution. Compressed sensing found direct applications in digital holography for microscopy. Indeed, the wave propagation phenomenon in free space mixes in a natural way the spatial distribution of point sources from the 3-dimensional scene. As the 3-dimensional scene is mapped to a 2-dimensional hologram, the hologram samples form a compressed representation of the scene as well. This overview paper discusses contributions in the field of compressed digital holography at the micro scale. Then, an outreach on future extensions towards the real-size macro scale is discussed. Thanks to advances in sensor technologies, increasing computing power and the recent improvements in sparse digital signal processing, holographic modalities are on the verge of practical high-quality visualization at a macroscopic scale where much higher resolution holograms must be acquired and processed on the computer.

  17. Direct-to-digital holography and holovision

    DOEpatents

    Thomas, Clarence E.; Baylor, Larry R.; Hanson, Gregory R.; Rasmussen, David A.; Voelkl, Edgar; Castracane, James; Simkulet, Michelle; Clow, Lawrence

    2000-01-01

    Systems and methods for direct-to-digital holography are described. An apparatus includes a laser; a beamsplitter optically coupled to the laser; a reference beam mirror optically coupled to the beamsplitter; an object optically coupled to the beamsplitter, a focusing lens optically coupled to both the reference beam mirror and the object; and a digital recorder optically coupled to the focusing lens. A reference beam is incident upon the reference beam mirror at a non-normal angle, and the reference beam and an object beam are focused by the focusing lens at a focal plane of the digital recorder to form an image. The systems and methods provide advantages in that computer assisted holographic measurements can be made.

  18. Very high-speed digital holography

    NASA Astrophysics Data System (ADS)

    Pérez López, Carlos; Mendoza Santoyo, Fernando; Rodríguez Vera, Ramón; Moreno, David; Barrientos, Bernardino

    2006-08-01

    It is reported for the first time the use of a high speed camera in digital holography with an out of plane sensitivity. The camera takes the image plane holograms of a cw laser illuminated rectangular framed polyester material at a rate of 5000 per second, that is a spacing of 200 microseconds between holograms, and 512 by 500 pixels at 10 bit resolution. The freely standing object has a random movement due to non controlled environmental air currents. As is usual with this technique each digital hologram is Fourier processed in order to obtain upon comparison with a consecutive digital hologram the phase map of the displacement. High quality results showing the amplitude and direction of the random movement are presented.

  19. Lensless multispectral digital in-line holographic microscope.

    PubMed

    Ryle, James P; McDonnell, Susan; Sheridan, John T

    2011-12-01

    An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.

  20. Lensless multispectral digital in-line holographic microscope

    NASA Astrophysics Data System (ADS)

    Ryle, James P.; McDonnell, Susan; Sheridan, John T.

    2011-12-01

    An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.

  1. Uncertainty-managed phase-shifting digital holography.

    PubMed

    Hahn, Joonku; Kim, Hwi

    2012-11-01

    Phase-shifting digital holography is a digital measurement technology of a complex optical field profile that uses focal plane array detectors without the loss of bandwidth. It has been known that the accuracy of phase-shifting digital holography is limited mainly by the phase tolerance of reference. In this Letter, it is revealed that the uncertainty in an expected signal is highly dependent on the phase of the signal itself, as well as the phase tolerance of the reference. Based on the uncertainty analysis, we propose a novel scheme of phase-shifting digital holography that exploits an uncertainty property to enhance the measurement accuracy even under significant reference phase tolerance.

  2. Geometric phase shifting digital holography.

    PubMed

    Jackin, Boaz Jessie; Narayanamurthy, C S; Yatagai, Toyohiko

    2016-06-01

    A new phase shifting digital holographic technique using a purely geometric phase in Michelson interferometric geometry is proposed. The geometric phase in the system does not depend upon either optical path length or wavelength, unlike dynamic phase. The amount of geometric phase generated is controllable through a rotating wave plate. The new approach has unique features and major advantages in holographic measurement of transparent and reflecting three-dimensional (3D) objects. Experimental results on surface shape measurement and imaging of 3D objects are presented using the proposed method.

  3. Wavelet image processing applied to optical and digital holography: past achievements and future challenges

    NASA Astrophysics Data System (ADS)

    Jones, Katharine J.

    2005-08-01

    The link between wavelets and optics goes back to the work of Dennis Gabor who both invented holography and developed Gabor decompositions. Holography involves 3-D images. Gabor decompositions involves 1-D signals. Gabor decompositions are the predecessors of wavelets. Wavelet image processing of holography, both optical holography and digital holography, will be examined with respect to past achievements and future challenges.

  4. Study of key technology of ghost imaging via compressive sensing for a phase object based on phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Leihong, Zhang; Dong, Liang; Bei, Li; Zilan, Pan; Dawei, Zhang; Xiuhua, Ma

    2015-07-01

    In this article, the algorithm of compressing sensing is used to improve the imaging resolution and realize ghost imaging via compressive sensing for a phase object based on the theoretical analysis of the lensless Fourier imaging of the algorithm of ghost imaging based on phase-shifting digital holography. The algorithm of ghost imaging via compressive sensing based on phase-shifting digital holography uses the bucket detector to measure the total light intensity of the interference and the four-step phase-shifting method is used to obtain the total light intensity of differential interference light. The experimental platform is built based on the software simulation, and the experimental results show that the algorithm of ghost imaging via compressive sensing based on phase-shifting digital holography can obtain the high-resolution phase distribution figure of the phase object. With the same sampling times, the phase clarity of the phase distribution figure obtained by the algorithm of ghost imaging via compressive sensing based on phase-shifting digital holography is higher than that obtained by the algorithm of ghost imaging based on phase-shift digital holography. In this article, this study further extends the application range of ghost imaging and obtains the phase distribution of the phase object.

  5. Remote metrology by comparative digital holography

    SciTech Connect

    Baumbach, Torsten; Osten, Wolfgang; Kopylow, Christoph von; Jueptner, Werner

    2006-02-10

    A method for the remote comparison of objects with regard to their shape or response to a load is presented. The method allows interferometric sensitivity for comparing objects with different microstructure. In contrast to the well-known incoherent techniques based on inverse fringe projection this new approach uses the coherent optical wave field of the master object as a mask for the illumination of the sample object. The coherent mask is created by digital holography to allow instant access to the complete optical information of the master object at any place desired. The mask is reconstructed by a spatial light modulator (SLM). The optical reconstruction of digital holograms with SLM technology allows modification of reconstructed wavefronts with respect to improvement of image quality, the skilled introduction of additional information about the object (augmented reality), and the alignment of the master and test object.

  6. Lensless digital holographic microscope using in-line configuration and laser diode illumination

    NASA Astrophysics Data System (ADS)

    Göring, Lena; Finkeldey, Markus; Adinda-Ougba, Adamou; Gerhardt, Nils C.; Hofmann, Martin

    2017-03-01

    In this paper we present a lensless transmission digital holographic microscope for the investigation of transparent samples. The setup consists of a laser diode, an object positioned on a cover slip and a CMOS sensor. We use a laser diode for illumination which emits a divergent beam and acts as a point source, so that additional components such as a pinhole are not required. The laser diode is operated below the lasing threshold to decrease the coherence length and thus to reduce speckle noise. Due to the compact and small size of the setup, it requires minimized effort for applications in field operation. The lensless setup was characterized by using an USAF-target for determining the resolution of the system which is 2.2 μm. In the following, transparent or semitransparent samples are investigated. Microstructured plastic samples are placed on the specimen holder and characterized by the holographic microscope. By applying the angular spectrum method on the recorded images, we are able to reconstruct the investigated objects. The in-line geometry of the setup facilitates the simplicity of the setup but also induces optical errors, for instance twin images. Twin images superimpose with the object's signal and require additional numerical reconstruction algorithms. For reducing the effect of the twin image problem, we apply an iterative phase retrieval algorithm. In the conclusion, we discuss the resolution and quality of the recorded images and evaluate the numerical reconstruction process.

  7. Multispectral lensless digital holographic microscope: imaging MCF-7 and MDA-MB-231 cancer cell cultures

    NASA Astrophysics Data System (ADS)

    Ryle, James P.; Molony, Karen M.; McDonnell, Susan; Naughton, Thomas J.; Sheridan, John T.

    2009-08-01

    Digital holography is the process where an object's phase and amplitude information is retrieved from intensity images obtained using a digital camera (e.g. CCD or CMOS sensor). In-line digital holographic techniques offer full use of the recording device's sampling bandwidth, unlike off-axis holography where object information is not modulated onto carrier fringes. Reconstructed images are obscured by the linear superposition of the unwanted, out of focus, twin images. In addition to this, speckle noise degrades overall quality of the reconstructed images. The speckle effect is a phenomenon of laser sources used in digital holographic systems. Minimizing the effects due to speckle noise, removal of the twin image and using the full sampling bandwidth of the capture device aids overall reconstructed image quality. Such improvements applied to digital holography can benefit applications such as holographic microscopy where the reconstructed images are obscured with twin image information. Overcoming such problems allows greater flexibility in current image processing techniques, which can be applied to segmenting biological cells (e.g. MCF-7 and MDA-MB- 231) to determine their overall cell density and viability. This could potentially be used to distinguish between apoptotic and necrotic cells in large scale mammalian cell processes, currently the system of choice, within the biopharmaceutical industry.

  8. Applications of digital holography to measurements and optical characterization

    NASA Astrophysics Data System (ADS)

    Sang, Xinzhu; Yu, Chongxiu; Yu, Miao; Hsu, Dashsiung

    2011-09-01

    With recent advances in high-speed computer and video capture technology, holographic films used in classical holography can be replaced with charged-coupled devices (CCD) and complementary metal-oxide-semiconductor (CMOS) image sensors to record and numerically reconstruct a hologram, which is now known as digital holography. Digital holography introduces something new to optical science. Wet chemical processing and other time-consuming procedures can be removed, so numerical recording and reconstruction can be realized in almost real time. It allows us to characterize the phase of a light field as well the intensity, and so the whole wave field can be measured and stored in a computer. Digital holography is expanding applications of holography and becoming a scientific and technological tool. Its use has now increased for measuring amplitude and the phase of object waves, displacement and three dimensional shape, particle distributions and motions, characterization of the refractive index and biological tissues, and vibration analysis, etc. Here, basic principles of digital holography for optical measurement and characterization are described. Taking into consideration the rapid advance in CCD and CMOS sensors as the background, the state-of-the-art applications of digital holography to optical measurement and characterization are presented.

  9. High speed 3D surface inspection with digital holography

    NASA Astrophysics Data System (ADS)

    Brunn, Andreas; Aspert, Nicolas; Cuche, Etienne; Emery, Yves; Ettemeyer, Andreas

    2013-01-01

    Digital holography has proven its ability to acquire high accuracy full field 3D data with one single image acquisition. This means that in principle this technique offers the chance to perform 3D serial inspection processes, as well. However, one limitation in digital holography is its limited ability to measure rough surfaces. In the presence of rough surfaces, the magnification of the image has to be increased to capture the required phase information on each camera pixel. However, this leads to significant reduction of inspection speed. If low magnification is selected, the rough surface produces speckles which cannot be treated properly by digital holography algorithms. In this paper, we describe the extension of digital holography to rough surface applications using speckle interferometry technique. This technique is capable of fast inspection of rough surfaces with sub-micrometer accuracy. The principle of this approach is shown and a practical application for 3D surface inspection of wafer cutting processes is given.

  10. Phase-shifting color digital holography.

    PubMed

    Yamaguchi, Ichirou; Matsumura, Tatsuki; Kato, Jun-Ichi

    2002-07-01

    Digital holography with a three-wavelength laser and a color CCD has been demonstrated. With the phase shifting of the reference beam, in-line holograms for three wavelengths are recorded simultaneously for derivation of the complex amplitude at each wavelength, and then the three monochromatic images are reconstructed and combined into full-color images in the computer. Laser power variation for wavelengths can be compensated for in the reconstruction process. We have compared the images reconstructed by two algorithms using a single Fourier transformation and a convolution with each other by both experiments and numerical simulations. Phase-shifting errors arising at two of the three wavelengths have proved not to cause serious deterioration of the reconstructed images.

  11. Incoherent digital holography with phase-only spatial light modulators

    NASA Astrophysics Data System (ADS)

    Rosen, Joseph; Kelner, Roy; Kashter, Yuval

    2015-10-01

    Today, spatial light modulators (SLMs) offer the world of digital holography a robust technology that can be incorporated into hologram recorders. This review surveys recent developments related to the role of SLMs in a family of incoherent digital hologram recorders termed Fresnel incoherent correlation holography (FINCH). Two systems branching out from FINCH, and discussed herein, are a confocal version of FINCH and a synthetic aperture FINCH-based system.

  12. Compact lensless digital holographic microscopy using a curved mirror for an enlarged working distance.

    PubMed

    Shi, Ervin Cheng; Ng, Joshua Jeremiah; Lim, Choo Min; Qu, Weijuan

    2016-05-10

    Digital holographic microscopy (DHM) has a wide range of applications from the analysis of microelectronic mechanical systems (MEMS) to the measurement of cells. We intend on making the system more compact to improve the portability of the device. A concave mirror has been presented to be used in a lensless DHM system to effectively enlarge the working distance and at the same time maintain the compact size of the whole system. A theoretical analysis of the phase compensation between the object wave and the wave reflected from curved reference mirrors is given. Experimental demonstrations of the curved reference mirrors used in the DHM system have been obtained to support our idea. This would change the overall size and adaptability of the DHM system and provide a better understanding of the effects of phase reflected off a curved mirror.

  13. Automatic three-dimensional tracking of particles with high-numerical-aperture digital lensless holographic microscopy.

    PubMed

    Restrepo, John F; Garcia-Sucerquia, Jorge

    2012-02-15

    We present an automatic procedure for 3D tracking of micrometer-sized particles with high-NA digital lensless holographic microscopy. The method uses a two-feature approach to search for the best focal planes and to distinguish particles from artifacts or other elements on the reconstructed stream of the holograms. A set of reconstructed images is axially projected onto a single image. From the projected image, the centers of mass of all the reconstructed elements are identified. Starting from the centers of mass, the morphology of the profile of the maximum intensity along the reconstruction direction allows for the distinguishing of particles from others elements. The method is tested with modeled holograms and applied to automatically track micrometer-sized bubbles in a sample of 4 mm3 of soda.

  14. Second-harmonic illumination to enhance multispectral digital lensless holographic microscopy.

    PubMed

    Mendoza-Yero, Omel; Carbonell-Leal, Miguel; Lancis, Jesús; Garcia-Sucerquia, Jorge

    2016-03-01

    Multispectral digital lensless holographic microscopy (MDLHM) operating with second-harmonic illumination is shown. Added to the improvement of the spatial resolution of the previously reported MDLHM operating with near-infrared illumination, this second-harmonic MDLHM shows promise as a tool to study the behavior of biological samples under a broad spectral illumination. This illumination is generated by focusing a highly spatially coherent ultrashort pulsed radiation into an uncoated Type 1 β-BaB2O4 (BBO) nonlinear crystal. The second-harmonic MDLHM allows achieving multispectral images of biological samples with enhanced micrometer spatial resolution. The illumination wavelength of the second-harmonic MDLHM can be tuned by displacing a focusing optics with respect to a pinhole; spatially resolved information at different wavelengths of the sample can then be retrieved.

  15. Color and monochrome lensless on-chip imaging of Caenorhabditis elegans over a wide field-of-view.

    PubMed

    Isikman, Serhan O; Sencan, Ikbal; Mudanyali, Onur; Bishara, Waheb; Oztoprak, Cetin; Ozcan, Aydogan

    2010-05-07

    We demonstrate color and monochrome on-chip imaging of Caenorhabditis elegans samples over a wide field-of-view using incoherent lensless in-line holography. Digital reconstruction of the recorded lensless holograms rapidly creates the C. elegans images within <1 s over a field-of-view of >24 mm2. By digitally combining the reconstructed images at three different wavelengths (red, green and blue), color images of dyed samples are also acquired. This wide field-of-view and compact on-chip imaging modality also permits straightforward integration with microfluidic systems.

  16. Double-sideband filter for digital holography

    NASA Astrophysics Data System (ADS)

    Ramírez, Claudio; Lizana, Angel; Iemmi, Claudio; Campos, Juan

    2017-02-01

    Nowadays, digital holographic systems are based on two main optical schemes: off-axis (OA) and inline (IL) holographic systems. In OA set-ups, the reference and the object beams present a relative angle at the registration plane. Thus, a real image of the object can be obtained without the influence of conjugated images by performing a spatial filtering at the reconstructed plane. IL configurations are less sensitive to vibrations and air flows than OA configurations, but the undesired influence of conjugated images in the final hologram is not avoided. To overcome this limitation, a number of IL based methods have been proposed. One interesting approach is the phase-shifting technique, which leads to efficient holograms for IL applications. However, due to the time-sequential nature of this technique, it is somewhat inappropriate for dynamic processes. We present a new method, for IL digital holography, based on a doublesideband (DSB) filter. This method not only removes the conjugate images in the reconstruction process but also reduces the distortions that usually appear when using single-sideband filters. Moreover, it is only time-limited by the acquisition time of the CCD camera. The appropriateness of the technique to be applied in dynamic processes was tested for the tracking of micro-particles. To this aim, particle holographic images were obtained by using the DSB method and afterwards processed with digital picture recognition methods, this allowing us to accurately track the spatial position of the particles. By using this approach, the instantaneous trajectory and velocity described by glass microspheres in movement were experimentally determined

  17. Compressive Fresnel digital holography using Fresnelet based sparse representation

    NASA Astrophysics Data System (ADS)

    Ramachandran, Prakash; Alex, Zachariah C.; Nelleri, Anith

    2015-04-01

    Compressive sensing (CS) in digital holography requires only very less number of pixel level detections in hologram plane for accurate image reconstruction and this is achieved by exploiting the sparsity of the object wave. When the input object fields are non-sparse in spatial domain, CS demands a suitable sparsification method like wavelet decomposition. The Fresnelet, a suitable wavelet basis for processing Fresnel digital holograms is an efficient sparsifier for the complex Fresnel field obtained by the Fresnel transform of the object field and minimizes the mutual coherence between sensing and sparsifying matrices involved in CS. The paper demonstrates the merits of Fresnelet based sparsification in compressive digital Fresnel holography over conventional method of sparsifying the input object field. The phase shifting digital Fresnel holography (PSDH) is used to retrieve the complex Fresnel field for the chosen problem. The results are presented from a numerical experiment to show the proof of the concept.

  18. Coaxial waveguide mode reconstruction and analysis with THz digital holography.

    PubMed

    Wang, Xinke; Xiong, Wei; Sun, Wenfeng; Zhang, Yan

    2012-03-26

    Terahertz (THz) digital holography is employed to investigate the properties of waveguides. By using a THz digital holographic imaging system, the propagation modes of a metallic coaxial waveguide are measured and the mode patterns are restored with the inverse Fresnel diffraction algorithm. The experimental results show that the THz propagation mode inside the waveguide is a combination of four modes TE₁₁, TE₁₂, TM₁₁, and TM₁₂, which are in good agreement with the simulation results. In this work, THz digital holography presents its strong potential as a platform for waveguide mode charactering. The experimental findings provide a valuable reference for the design of THz waveguides.

  19. Integrated phase-shifting digital holography using statistical approach

    NASA Astrophysics Data System (ADS)

    Yoshikawa, Nobukazu; Kajihara, Kazuki; Shiratori, Takaaki

    2014-11-01

    We propose integrated phase-shifting digital holography using statistical approach. In the integrated phase-shifting scheme, the phase shifts are generated by a reference mirror moving with a uniform velocity, and sequential phaseshifted holograms are captured. Therefore, there is no wait time for stabilization, which offers some advantages such as short measurement time, high phase stability and high noise tolerance. The proposed method does not require precise control and calibration of the phase shifter and synchronization between the phase shifter and the digital image sensor. Therefore, the practical digital holography system with high accuracy can be implemented at low cost.

  20. Parallel two-step phase-shifting digital holography.

    PubMed

    Awatsuji, Yasuhiro; Tahara, Tatsuki; Kaneko, Atsushi; Koyama, Takamasa; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2008-07-01

    We propose a parallel two-step phase-shifting digital holography technique capable of instantaneous measurement of three-dimensional objects, with a view toward measurement of dynamically moving objects. The technique is based on phase-shifting interferometry. The proposed technique carries out the two-step phase-shifting method at one time and can be optically implemented by using a phase-shifting array device located in the reference beam. The array device has a periodic two-step phase distribution, and its configuration is simplified compared with that required for three-step and four-step parallel phase-shifting digital holographies. Therefore the optical system of the proposed technique is more suitable for the realization of a parallel phase-shifting digital holography system. We conduct both a numerical simulation and a preliminary experiment in the proposed technique. The results of the simulation and the experiment agree well with those of sequential phase-shifting digital holography, and results are superior to those obtained by conventional digital holography using the Fresnel transform alone. Thus the effectiveness of the proposed technique is verified.

  1. Digital holography system for undergraduate student laboratory

    NASA Astrophysics Data System (ADS)

    Buranasiri, P.; Plaipichit, S.; Yindeesuk, W.; Yoshimori, K.

    2015-07-01

    In this paper, we discuss the digital holography (DH) experiment in our optical and communication laboratory course for undergraduate students at Physics department, KMITL. The purposes of DH experiment are presenting our students the meaning and advantage of DH and its applications. The Gabor configurations of in-line DH has been set up for recording a number of samples, which were placed on different distances, simultaneously. Then, the images of all objects have been numerical reconstructed by using computer. The students have been learned that all of reconstructed images have been got from only one time recording, while using the conventional recording technique, sharp images of different objects have been gotten from different recording time. The students also have been learned how to use DH technique for investigation some different kinds of samples on their own of interested such as a human hair or a fingerprint. In our future work, our DH system will be developed to be a portable apparatus for easily showing to children in different areas.

  2. Single-exposure two-dimensional superresolution in digital holography using a vertical cavity surface-emitting laser source array.

    PubMed

    Granero, Luis; Zalevsky, Zeev; Micó, Vicente

    2011-04-01

    We present a new implementation capable of producing two-dimensional (2D) superresolution (SR) imaging in a single exposure by aperture synthesis in digital lensless Fourier holography when using angular multiplexing provided by a vertical cavity surface-emitting laser source array. The system performs the recording in a single CCD snapshot of a multiplexed hologram coming from the incoherent addition of multiple subholograms, where each contains information about a different 2D spatial frequency band of the object's spectrum. Thus, a set of nonoverlapping bandpass images of the input object can be recovered by Fourier transformation (FT) of the multiplexed hologram. The SR is obtained by coherent addition of the information contained in each bandpass image while generating an enlarged synthetic aperture. Experimental results demonstrate improvement in resolution and image quality.

  3. Dynamic imaging through turbid media based on digital holography.

    PubMed

    Li, Shiping; Zhong, Jingang

    2014-03-01

    Imaging through turbid media using visible or IR light instead of harmful x ray is still a challenging problem, especially in dynamic imaging. A method of dynamic imaging through turbid media using digital holography is presented. In order to match the coherence length between the dynamic object wave and the reference wave, a cw laser is used. To solve the problem of difficult focusing in imaging through turbid media, an autofocus technology is applied. To further enhance the image contrast, a spatial filtering technique is used. A description of digital holography and experiments of imaging the objects hidden in turbid media are presented. The experimental result shows that dynamic images of the objects can be achieved by the use of digital holography.

  4. Accurate reconstruction of digital holography using frequency domain zero padding

    NASA Astrophysics Data System (ADS)

    Shin, Jun Geun; Kim, Ju Wan; Lee, Jae Hwi; Lee, Byeong Ha

    2017-04-01

    We propose an image reconstruction method of digital holography for getting more accurate reconstruction. Digital holography provides both the light amplitude and the phase of a specimen through recording the interferogram. Since the Fresenl diffraction can be efficiently implemented by the Fourier transform, zero padding technique can be applied to obtain more accurate information. In this work, we report the method of frequency domain zero padding (FDZP). Both in computer-simulation and in experiment made with a USAF 1951 resolution chart and target, the FDZD gave the more accurate rconstruction images. Even though, the FDZD asks more processing time, with the help of graphics processing unit (GPU), it can find good applications in digital holography for 3-D profile imaging.

  5. Three-dimensional image fusion by use of multiwavelength digital holography.

    PubMed

    Javidi, Bahram; Ferraro, Pietro; Hong, Seung-Hyun; De Nicola, Sergio; Finizio, Andrea; Alfieri, Domenico; Pierattini, Giovanni

    2005-01-15

    We present three-dimensional (3D) image fusion by use of digital holography. We demonstrate experimentally that, through the image fusion technique with multiresolution wavelet decomposition, it is possible to increase the details and contrast of 3D reconstructed images obtained by multiwavelength digital holography. Although there is substantial activity in the fields of image fusion and holography, to the best of our knowledge, this is the first report of 3D image fusion by use of digital holography.

  6. Shape and 3D acoustically induced vibrations of the human eardrum characterized by digital holography

    NASA Astrophysics Data System (ADS)

    Khaleghi, Morteza; Furlong, Cosme; Cheng, Jeffrey Tao; Rosowski, John J.

    2014-07-01

    The eardrum or Tympanic Membrane (TM) transfers acoustic energy from the ear canal (at the external ear) into mechanical motions of the ossicles (at the middle ear). The acousto-mechanical-transformer behavior of the TM is determined by its shape and mechanical properties. For a better understanding of hearing mysteries, full-field-of-view techniques are required to quantify shape, nanometer-scale sound-induced displacement, and mechanical properties of the TM in 3D. In this paper, full-field-of-view, three-dimensional shape and sound-induced displacement of the surface of the TM are obtained by the methods of multiple wavelengths and multiple sensitivity vectors with lensless digital holography. Using our developed digital holographic systems, unique 3D information such as, shape (with micrometer resolution), 3D acoustically-induced displacement (with nanometer resolution), full strain tensor (with nano-strain resolution), 3D phase of motion, and 3D directional cosines of the displacement vectors can be obtained in full-field-ofview with a spatial resolution of about 3 million points on the surface of the TM and a temporal resolution of 15 Hz.

  7. High temperature measurements of martensitic transformations using digital holography.

    PubMed

    Thiesing, Benjamin P; Mann, Christopher J; Dryepondt, Sebastien

    2013-07-01

    During thermal cycling of nickel-aluminum-platinum (NiAlPt) and single crystal iron-chromium-nickel (FeCrNi) alloys, the structural changes associated with the martensite to austenite phase transformation were measured using dual-wavelength digital holography. Real-time in situ measurements reveal the formation of striations within the NiAlPt alloy at 70°C and the FeCrNi alloy at 520°C. The results demonstrate that digital holography is an effective technique for acquiring noncontact, high precision information of the surface evolution of alloys at high temperatures.

  8. Parallel phase-shifting digital holography using LCOS-SLM

    NASA Astrophysics Data System (ADS)

    Lin, Miao; Nitta, Kouichi; Matoba, Osamu; Awatsuji, Yasuhiro

    2013-12-01

    Parallel phase-shifting digital holography (PPSDH) method can record dynamic three-dimensional events with higher spatial resolution than that of off-axis digital holography. In PPSDH, all amount of phase retardations are included in a multiplexed hologram and can be optically implemented by using a phase-mode spatial light modulator (SLM) located in the reference beam. The SLM can also compensate optical aberration caused by lenses, beam splitters, and air fluctuation as in adaptive optics. In this presentation, we review our experimental results using stationary two-dimensional object using a liquid-crystal on Silicon (LCOS) SLM.

  9. Pixel resolution control in numerical reconstruction of digital holography.

    PubMed

    Yu, Lingfeng; Kim, Myung K

    2006-04-01

    A new method for resolution control in numerical reconstruction of digital holography is proposed. The wave field on a tilted or vertical plane can be reconstructed without being subject to the minimum object-to-hologram distance requirement, and the pixel resolution can be easily controlled by adjusting the position of a transitional plane. The proposed method solves the problem of pixel resolution control for small object-to-hologram distances and is especially useful for multicolor, multiwavelength digital holography and metrological applications. Experimental results are presented to verify the idea.

  10. High Temperature Measurements Of Martensitic transformations Using Digital Holography

    SciTech Connect

    Thiesing, Benjamin; Mann, Christopher J; Dryepondt, Sebastien N

    2013-01-01

    During thermal cycling of nickel-aluminum-platinum (NiAlPt) and single crystal Fe-15Cr-15Ni alloys, the structural changes associated with the martensite to austenite phase transformation were measured using dual-wavelength digital holography. Real-time in-situ measurements reveal the formation of striations within the NiPtAl alloy at 70 C and the FeCrNi alloy at 520 C. The results demonstrate that digital holography is an effective technique for acquiring non-contact, high precision information of the surface evolution of alloys at high temperatures.

  11. Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging.

    PubMed

    Micó, Vicente; Zalevsky, Zeev

    2010-01-01

    Digital in-line holographic microscopy (DIHM) is a modern approach capable of achieving micron-range lateral and depth resolutions in three-dimensional imaging. DIHM in combination with numerical imaging reconstruction uses an extremely simplified setup while retaining the advantages provided by holography with enhanced capabilities derived from algorithmic digital processing. We introduce superresolved DIHM incoming from time and angular multiplexing of the sample spatial frequency information and yielding in the generation of a synthetic aperture (SA). The SA expands the cutoff frequency of the imaging system, allowing submicron resolutions in both transversal and axial directions. The proposed approach can be applied when imaging essentially transparent (low-concentration dilutions) and static (slow dynamics) samples. Validation of the method for both a synthetic object (U.S. Air Force resolution test) to quantify the resolution improvement and a biological specimen (sperm cells biosample) are reported showing the generation of high synthetic numerical aperture values working without lenses.

  12. Watermarking of three-dimensional objects by digital holography

    NASA Astrophysics Data System (ADS)

    Kishk, Sherif; Javidi, Bahram

    2003-02-01

    We present an optical method for information watermarking of three-dimensional (3D) objects by digital holography. A hidden image is embedded by double phase encoding in a phase-shift digital hologram of the 3D object. We decode the watermarked hologram to reconstruct the hidden image and the 3D object. We use either the entire hologram or a part of it to decode the hidden image. Experiments are presented to illustrate the ability to recover both the 3D object and the decoded hidden image. Digital holograms of the 3D object are obtained by optical experiments. The watermarking process, 3D object reconstruction, and hidden image recovery are performed digitally. To the best of our knowledge, this is the first report of 3D object watermarking by use of a phase encoding technique and digital holography.

  13. Digital holography and 3D imaging: introduction to feature issue.

    PubMed

    Kim, Myung K; Hayasaki, Yoshio; Picart, Pascal; Rosen, Joseph

    2013-01-01

    This feature issue of Applied Optics on Digital Holography and 3D Imaging is the sixth of an approximately annual series. Forty-seven papers are presented, covering a wide range of topics in phase-shifting methods, low coherence methods, particle analysis, biomedical imaging, computer-generated holograms, integral imaging, and many others.

  14. A digital holography set-up for 3D vortex flow dynamics

    NASA Astrophysics Data System (ADS)

    Lebon, Benoît; Perret, Gaële; Coëtmellec, Sébastien; Godard, Gilles; Gréhan, Gérard; Lebrun, Denis; Brossard, Jérôme

    2016-06-01

    In the present paper, a digital in-line holography (DIH) set-up, with a converging beam, is used to take three-dimensional (3D) velocity measurements of vortices. The vortices are formed periodically at the edges of a submerged horizontal plate submitted to regular waves. They take the form of vortex filaments that extend from side to side of the channel. They undergo strongly three-dimensional instability mechanisms that remain very complicated to characterize experimentally. The experiments are performed in a 10 × 0.3 × 0.3 m3 wave flume. The DIH set-up is performed using a modulated laser diode emitting at the wavelength of 640 nm and a lensless CCD camera. The beam crosses the channel side to side. To reveal the flow dynamics, 30-μm hydrogen bubbles are generated at the edge of the plate to serve as tracers. Their locations are recorded on the holograms multiple times to access the dynamics of the flow. This method leads to an accuracy in the order of 100 μm on the axial location. Those measurements have been validated with stereo-PIV measurements. A very good agreement is found on time-averaged velocity fields between the two techniques.

  15. Extreme ultraviolet digital in-line holography using a tabletop source.

    PubMed

    Zürch, Michael; Spielmann, Christian

    2015-07-01

    Digital in-line holography (DIH) offers fast, lensless, and aberration-free imaging with diffraction-limited resolution and inherently combines phase- and amplitude-contrast imaging, as well as three-dimensional imaging. Extending this technique to shorter wavelengths allows increasing the achievable spatial and phase-contrast resolution, as well as accessing material parameters not accessible in the optical domain. In this paper, we report on DIH experiments conducted with a coherent tabletop ultrafast high harmonic source operated at 38 nm wavelength. Applying a twin-image-free reconstruction scheme optimized for highly absorbing samples, we were able to demonstrate the phase-contrast imaging of silicon nitride sheets of 15 nm thickness and the use of the strong absorption of extreme ultraviolet in matter for amplitude-contrast imaging of thin films with spatial resolution below 1 μm. High-resolution morphology determination in combination with phase-contrast imaging is of special importance in thin-film characterization and applications arising thereof.

  16. Single-shot phase-shifting incoherent digital holography

    NASA Astrophysics Data System (ADS)

    Tahara, Tatsuki; Kanno, Takeya; Arai, Yasuhiko; Ozawa, Takeaki

    2017-06-01

    We propose single-shot incoherent digital holography in which a single-path in-line configuration and phase-shifting interferometry are adopted. Space-division multiplexing and polarization states of the waves are utilized to implement parallel phase-shifting holography. A single-path setup in parallel phase-shifting is constructed to capture an incoherent hologram easily with a compact system. An instantaneous and three-dimensional (3D) object image is obtained without undesired diffraction waves using parallel phase-shifting. The validity of the proposed technique is experimentally demonstrated for both transparent and reflective objects.

  17. Imaging a vibrating object by Sideband Digital Holography.

    PubMed

    Joud, F; Laloë, F; Atlan, M; Hare, J; Gross, M

    2009-02-16

    We obtain quantitative measurements of the oscillation amplitude of vibrating objects by using sideband digital holography. The frequency sidebands on the light scattered by the object, shifted by n times the vibration frequency, are selectively detected by heterodyne holography, and images of the object are calculated for different orders n. Orders up to n = 120 have been observed, allowing the measurement of amplitudes of oscillation that are significantly larger than the optical wavelength. Using the positions of the zeros of intensity for each value of n, we reconstruct the shape of vibration the object.

  18. Numerical evaluation of the limit of concentration of colloidal samples for their study with digital lensless holographic microscopy.

    PubMed

    Restrepo, John F; Garcia-Sucerquia, Jorge

    2013-01-01

    The number of colloidal particles per unit of volume that can be imaged correctly with digital lensless holographic microscopy (DLHM) is determined numerically. Typical in-line DLHM holograms with controlled concentration are modeled and reconstructed numerically. By quantifying the ratio of the retrieved particles from the reconstructed hologram to the number of the seeding particles in the modeled intensity, the limit of concentration of the colloidal suspensions up to which DLHM can operate successfully is found numerically. A new shadow density parameter for spherical illumination is defined. The limit of performance of DLHM is determined from a graph of the shadow density versus the efficiency of the microscope.

  19. Parallel optical-path-length-shifting digital holography.

    PubMed

    Awatsuji, Yasuhiro; Koyama, Takamasa; Tahara, Tatsuki; Ito, Kenichi; Shimozato, Yuki; Kaneko, Atsushi; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2009-12-01

    The authors propose an optical-path-length-shifting digital holography as a technique capable of single-shot recording of three-dimensional information of objects. With a single image sensor, the proposed technique can simultaneously record all of the holograms required for the in-line digital holography that reconstruct the image of an object from two intensity measurements at different planes. The technique can be optically implemented by using an optical-path-length-shifting array device located in the common path of the reference and object waves. The array device has periodic structure of two-step optical-path difference. The configuration of the array device of the proposed technique is simpler than the phase-shifting array device required for parallel phase-shifting digital holographies. Therefore, the optical system of the proposed technique is more suitable for the realization of a single-shot in-line digital holography system that removes the conjugate image from the reconstructed image. The authors conducted both a numerical simulation and a preliminary experiment of the proposed technique. The reconstructed images were quantitatively evaluated by using root mean squared error. In comparison to single-shot digital holography using the Fresnel transform alone, with the proposed technique the root mean squared errors of the technique were reduced to less than 1/6 in amplitude and 1/3 in phase. Also the results of the simulation and experiment agreed well with the images of an object. Thus the effectiveness of the proposed technique is verified.

  20. Single-shot phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Zhang, Jing; Xie, Yu; Li, Guifang; Ye, Yutang; Saleh, Bahaa E. A.

    2014-11-01

    We propose a digital holographic imaging system that is capable of recording/measuring the amplitude and phase of an optical wavefront in a single shot. Based on the principle of digital holography (DH), the proposed system uses a reference beam to interfere with the light field under investigation and digitally records/measures the in-phase and quadrature interference patterns. Single-shot in-phase and quadrature interference pattern recording is made possible by incorporating the technologies widely used in coherent optical communication. Specifically, a free-space optical 90-deg hybrid is employed to measure the complex (real and imaginary) optical field. We have constructed a single-shot phase-shifting digital holography system and experimentally demonstrated its operation.

  1. Quantitative phase imaging by three-wavelength digital holography

    SciTech Connect

    Mann, Christopher J; Bingham, Philip R; Tobin Jr, Kenneth William; Paquit, Vincent C

    2008-01-01

    Three-wavelength digital holography is applied to obtain surface height measurements over several microns of range, while simultaneously maintaining the low noise precision of the single wavelength phase measurement. The precision is preserved by the use of intermediate synthetic wavelength steps generated from the three wavelengths and the use of hierarchical optical phase unwrapping. As the complex wave-front of each wavelength can be captured simultaneously in one digital image, real-time performance is achievable.

  2. Quantitative phase imaging by three-wavelength digital holography.

    PubMed

    Mann, Christopher J; Bingham, Philip R; Paquit, Vincent C; Tobin, Kenneth W

    2008-06-23

    Three-wavelength digital holography is applied to obtain surface height measurements over several microns of range, while simultaneously maintaining the low noise precision of the single wavelength phase measurement. The precision is preserved by the use of intermediate synthetic wavelength steps generated from the three wavelengths and the use of hierarchical optical phase unwrapping. As the complex wave-front of each wavelength can be captured simultaneously in one digital image, real-time performance is achievable.

  3. One million fps phase measurement by digital holography

    NASA Astrophysics Data System (ADS)

    Xia, Peng; Awatsuji, Yasuhiro; Matoba, Osamu

    2017-02-01

    Parallel phase-shifting digital holography is one of digital holographic techniques and good at high-speed recording of moving object. The phase distribution of the object can be calculated from recorded hologram so that the technique has been actively applied in high-speed three-dimensional measurement. In this paper, we review a high-speed parallel phase-shifting digital holography system consists of a high-speed polarization-imaging camera (FASTCAM-SA5-P, Photoron, Inc.) and a Nd:YVO4 laser with 532 nm wavelength, 150 mW output power. In the experiment, a simple electrical discharging equipment was set for the fast object, and the electrical discharging phenomenon was successfully recorded at the rate of 25,000 fps, and 1,000,000 fps respectively.

  4. Resampling masks for phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Zhang, Wenhui; Cao, Liangcai; Zhang, Hao; Zong, Song; Jin, Guofan

    2016-10-01

    Holographic imaging is degraded severely by the speckle or incoherent noise. In this work, resampling mask method based on phase-shifting digital holography is proposed to reduce the reconstruction noise. The zero-order and the conjugate term of the hologram can be eliminated with four-step phase-shifting digital holography (PSDH). The complex amplitude of the object after propagation can be calculated by the phase-shifting algorithm. A phase-only spatial light modulator is used to realize PSDH in the experiment. The complex amplitude is encoded into several parts by resampling masks. A high quality reconstruction image with low noise is achieved through the superposition of the individually reconstruction of coded complex amplitudes. The experiment data go well with the asymptotic function. This method can be used for digital holographic imaging of the biological samples and microstructures. Experimental results prove the feasibility of this proposed method.

  5. Digital holography with a quadrature phase-shifting interferometer.

    PubMed

    Kiire, Tomohiro; Nakadate, Suezou; Shibuya, Masato

    2009-03-01

    An alternative method for digital holography using a quadrature phase-shifting interferometer for high-speed measurement is presented. We show that it has image quality equal to the four-bucket method. In addition, it requires fewer imaging devices. Two quadrature phase-shifting fringe patterns are acquired in each state of an object changed temporally. The phase calculation method with these four fringe patterns gives the phase distribution of the hologram. This digital phase hologram is reconstructed to yield an object image by the Fresnel transform using digital convolutions with the fast Fourier transform algorithm. Verification results of simulations and experiments are given.

  6. Linear programming phase unwrapping for dual-wavelength digital holography.

    PubMed

    Wang, Zhaomin; Jiao, Jiannan; Qu, Weijuan; Yang, Fang; Li, Hongru; Tian, Ailing; Asundi, Anand

    2017-01-20

    A linear programming phase unwrapping method in dual-wavelength digital holography is proposed and verified experimentally. The proposed method uses the square of height difference as a convergence standard and theoretically gives the boundary condition in a searching process. A simulation was performed by unwrapping step structures at different levels of Gaussian noise. As a result, our method is capable of recovering the discontinuities accurately. It is robust and straightforward. In the experiment, a microelectromechanical systems sample and a cylindrical lens were measured separately. The testing results were in good agreement with true values. Moreover, the proposed method is applicable not only in digital holography but also in other dual-wavelength interferometric techniques.

  7. Multiwavelength parallel phase-shifting digital holography using angular multiplexing.

    PubMed

    Tahara, Tatsuki; Ito, Yasunori; Lee, Yonghee; Xia, Peng; Inoue, Junichi; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2013-08-01

    We propose a single-shot digital holography for recording multiwavelength and complex amplitude information by using a single monochromatic image sensor. The zeroth-order wave and conjugate image in each wavelength are removed from a recorded single hologram by applying parallel phase-shifting interferometry. Angular multiplexing is utilized to record the complex amplitude of an object wave in each wavelength separately, and no color filter is required. The effectiveness of the proposed technique was experimentally verified.

  8. Three-dimensional microscopy with phase-shifting digital holography.

    PubMed

    Zhang, T; Yamaguchi, I

    1998-08-01

    We applied phase-shifting digital holography to microscopy by deriving the complex amplitude of light scattered from microscopic three-dimensional objects through a microscope objective by video camera recording, phase-shifting analysis, and computer reconstruction. This method requires no mechanical movement and provides a flexible display and quantitative evaluation of the reconstructed images. A theory of image formation and experimental verification with specimens are described.

  9. Holography at the U.S. Army Research Laboratory: Creating a Digital Hologram

    DTIC Science & Technology

    2012-09-01

    Holography at the U.S. Army Research Laboratory: Creating a Digital Hologram by Karl K. Klett, Jr., Neal Bambha, and Justin Bickford...MD 20783-1197 ARL-TR-6299 September 2012 Holography at the U.S. Army Research Laboratory: Creating a Digital Hologram Karl K. Klett...MM-YYYY) September 2012 2. REPORT TYPE Final 3. DATES COVERED (From - To) January 2012–Present 4. TITLE AND SUBTITLE Holography at the U.S

  10. Space-Bandwidth Capacity-Enhanced Digital Holography

    NASA Astrophysics Data System (ADS)

    Tahara, Tatsuki; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Matoba, Osamu; Kubota, Toshihiro

    2013-02-01

    We propose a single-shot digital holography in which the space bandwidth available for recording an object wave can be extended in an off-axis configuration. The key points of this technique are utilizing the periodicity of a digital signal and the undersampling, intentionally setting the aliasing to the recorded hologram, and conducting spatial-carrier phase-shifting interferometry and the Fourier transform method. The image quality for both large objects and fine structures can be improved by the keys. The effectiveness of the proposed technique was numerically and experimentally demonstrated. Then, the performance and the optimal angle condition were quantitatively analyzed.

  11. Dynamic modal characterization of musical instruments using digital holography.

    PubMed

    Demoli, Nazif; Demoli, Ivan

    2005-06-27

    This study shows that a dynamic modal characterization of musical instruments with membrane can be carried out using a low-cost device and that the obtained very informative results can be presented as a movie. The proposed device is based on a digital holography technique using the quasi-Fourier configuration and time-average principle. Its practical realization with a commercial digital camera and large plane mirrors allows relatively simple analyzing of big vibration surfaces. The experimental measurements given for a percussion instrument are supported by the mathematical formulation of the problem.

  12. Dynamic modal characterization of musical instruments using digital holography

    NASA Astrophysics Data System (ADS)

    Demoli, Nazif; Demoli, Ivan

    2005-06-01

    This study shows that a dynamic modal characterization of musical instruments with membrane can be carried out using a low-cost device and that the obtained very informative results can be presented as a movie. The proposed device is based on a digital holography technique using the quasi-Fourier configuration and time-average principle. Its practical realization with a commercial digital camera and large plane mirrors allows relatively simple analyzing of big vibration surfaces. The experimental measurements given for a percussion instrument are supported by the mathematical formulation of the problem.

  13. Twin removal in digital holography using diffuse illumination.

    PubMed

    Monaghan, David S; Kelly, Damien P; Pandey, Nitesh; Hennelly, Bryan M

    2009-12-01

    A method to numerically remove the twin image for inline digital holography, using multiple digital holograms, is discussed. Each individual hologram is recorded by using a statistically independent speckle field to illuminate the object. If the holograms are recorded in this manner and then numerically reconstructed, the twin image appears as a different speckle pattern in each of the reconstructions. By performing speckle-reduction techniques the presence of the twin image can be greatly reduced. A theoretical model is developed, and experimental results are presented that validate this approach. We show experimentally that the dc object intensity term can also be removed by using this technique.

  14. Noise reduction in digital lensless holographic microscopy by engineering the light from a light-emitting diode.

    PubMed

    Garcia-Sucerquia, Jorge

    2013-01-01

    By engineering the light from a light-emitting diode (LED) the noises present in digital lensless holographic microscopy (DLHM) are reduced. The partially coherent light from an LED is tailored to produce a spherical wavefront with limited coherence time and the spatial coherence needed by DLHM to work. DLHM with this engineered light source is used to image biological samples that cover areas of the order of mm(2). The ratio between the diameter of the area that is almost coherently illuminated to the diameter of the illumination area is utilized as parameter to quantify the performance of the DLHM with the engineered LED light source. Experimental results show that while the noises can be reduced effectively the spatial resolution can be kept in the micrometer range.

  15. Comparative analysis of the modified enclosed energy metric for self-focusing holograms from digital lensless holographic microscopy.

    PubMed

    Trujillo, Carlos; Garcia-Sucerquia, Jorge

    2015-06-01

    A comparative analysis of the performance of the modified enclosed energy (MEE) method for self-focusing holograms recorded with digital lensless holographic microscopy is presented. Notwithstanding the MEE analysis previously published, no extended analysis of its performance has been reported. We have tested the MEE in terms of the minimum axial distance allowed between the set of reconstructed holograms to search for the focal plane and the elapsed time to obtain the focused image. These parameters have been compared with those for some of the already reported methods in the literature. The MEE achieves better results in terms of self-focusing quality but at a higher computational cost. Despite its longer processing time, the method remains within a time frame to be technologically attractive. Modeled and experimental holograms have been utilized in this work to perform the comparative study.

  16. Talbot self-image effect in digital holography and its application to spectrometry.

    PubMed

    De Nicola, Sergio; Ferraro, Pietro; Coppola, Giuseppe; Finizio, Andrea; Pierattini, Giovanni; Grilli, Simonetta

    2004-01-01

    For the first time to the authors' knowledge, the Talbot effect has been observed and investigated in digital holography. By numerical reconstruction of holograms, the Talbot self-imaging phenomenon is observed by reconstruction of the amplitude of the image at different distances and (or) wavelengths. A simple spectrometer based on Talbot self-imaging in digital holography is proposed and demonstrated.

  17. Holography.

    ERIC Educational Resources Information Center

    Klein, H. Arthur

    Holography is a process which numbers among its many applications the creation of holograms--unique three dimensional photographs that show spatial relations and shifts just as they exist in reality. This book recounts the history of holography, tracing its development from Euclid's theory of light rays through Huygens' theory of wave motion to…

  18. Holography.

    ERIC Educational Resources Information Center

    Klein, H. Arthur

    Holography is a process which numbers among its many applications the creation of holograms--unique three dimensional photographs that show spatial relations and shifts just as they exist in reality. This book recounts the history of holography, tracing its development from Euclid's theory of light rays through Huygens' theory of wave motion to…

  19. Numerical suppression of zero-order image in digital holography.

    PubMed

    Chen, Gu-Liang; Lin, Ching-Yang; Kuo, Ming-Kuei; Chang, Chi-Ching

    2007-07-09

    This work describes a novel approach that adopts numerical operation to suppress the zero-order images of reconstruction in digital holography. The entire process needs only one digital hologram and keeps under control the intensity ratio of the object wave to reference wave in recording procedure. Also the performance of numerical suppression is simple and effective by subtracting the numerical generated intensity of the object and reference waves from the digital hologram. The experimental results demonstrate that the zero-order images of reconstruction can be suppressed completely and represents the satisfactory reconstructed image even if the distribution of the object wave is not uniform. Therefore this approach can simplify the procedure of phase-shifting digital holographic-based scheme involving multiple exposures. Moreover, the investigation of performance using the novel suppression approach is presented for proving the practical feasibility.

  20. Lensless microscope based on iterative in-line holographic reconstruction

    NASA Astrophysics Data System (ADS)

    Wu, Jigang

    2014-11-01

    We propose a lensless microscopic imaging technique based on iteration algorithm with known constraint for image reconstruction in digital in-line holography. In our method, we introduce a constraint on the sample plane as known part in the lensless microscopy for iteration algorithm in order to eliminate the twin-image effect of holography and thus lead to better performance on microscopic imaging. We evaluate our method by numerical simulation and built a prototype in-line holographic imaging system and demonstrated its capability by preliminary experiments. In our proposed setup, a carefully designed photomask used to hold the sample is under illumination of a coherent light source. The in-line hologram is then recorded by a CMOS sensor. In the reconstruction, the known information of the illumination beam and the photomask is used as constraints in the iteration process. The improvement of image quality because of suppression of twin-images can be clearly seen by comparing the images obtained by direct holographic reconstruction and our iterative method.

  1. In-line digital holography with double knife edge

    NASA Astrophysics Data System (ADS)

    Ramirez, Claudio; Iemmi, Claudio; Campos, Juan

    2015-06-01

    We study and test a new technique for in-line digital holography which avoids the formation of the conjugate images. Inline digital holography is based in a common path configuration. In this case, the hologram is produced by the interference between the reference wave front and the diffracted wave front by an almost transparent object. Twin images are obtained with obscured rings that difficult the determination of the best focusing plane. To avoid the conjugated image, the information of the magnitude and phase of the wave front are needed. In a recent work a new in-line digital holography technique was proposed. In this method the object is illuminated with a collimated wave front. A plane, close to the particles distribution is imaged onto a CCD by means of a convergent lens and at the same time, a knife edge is placed in the focal plane of the lens in order to block half of spatial frequency spectrum. In this way, by means of a numerical processing performed on the Fourier plane, it is possible to eliminate one of the components (real or conjugate) of the reconstructed images nevertheless it is observed a tiny deformation of the resulting hologram image. To compensate this effect, we propose a new configuration in which we implement the knife edge technique on both parts of the spectrum at the same time. Finally in the computer, we process the holograms to build one complete without deformation. This hologram is used to recover the wave front at different planes without the influence of the conjugate image.

  2. Three-dimensional vibrometry of the human eardrum with stroboscopic lensless digital holography.

    PubMed

    Khaleghi, Morteza; Furlong, Cosme; Ravicz, Mike; Cheng, Jeffrey Tao; Rosowski, John J

    2015-05-01

    The eardrum or tympanic membrane (TM) transforms acoustic energy at the ear canal into mechanical motions of the ossicles. The acousto-mechanical transformer behavior of the TM is determined by its shape, three-dimensional (3-D) motion, and mechanical properties. We have developed an optoelectronic holographic system to measure the shape and 3-D sound-induced displacements of the TM. The shape of the TM is measured with dual-wavelength holographic contouring using a tunable near IR laser source with a central wavelength of 780 nm. 3-D components of sound-induced displacements of the TM are measured with the method of multiple sensitivity vectors using stroboscopic holographic interferometry. To accurately obtain sensitivity vectors, a new technique is developed and used in which the sensitivity vectors are obtained from the images of a specular sphere that is being illuminated from different directions. Shape and 3-D acoustically induced displacement components of cadaveric human TMs at several excitation frequencies are measured at more than one million points on its surface. A numerical rotation matrix is used to rotate the original Euclidean coordinate of the measuring system in order to obtain in-plane and out-of-plane motion components. Results show that in-plane components of motion are much smaller (<20%) than the out-of-plane motions’ components.

  3. Three-dimensional vibrometry of the human eardrum with stroboscopic lensless digital holography

    NASA Astrophysics Data System (ADS)

    Khaleghi, Morteza; Furlong, Cosme; Ravicz, Mike; Cheng, Jeffrey Tao; Rosowski, John J.

    2015-05-01

    The eardrum or tympanic membrane (TM) transforms acoustic energy at the ear canal into mechanical motions of the ossicles. The acousto-mechanical transformer behavior of the TM is determined by its shape, three-dimensional (3-D) motion, and mechanical properties. We have developed an optoelectronic holographic system to measure the shape and 3-D sound-induced displacements of the TM. The shape of the TM is measured with dual-wavelength holographic contouring using a tunable near IR laser source with a central wavelength of 780 nm. 3-D components of sound-induced displacements of the TM are measured with the method of multiple sensitivity vectors using stroboscopic holographic interferometry. To accurately obtain sensitivity vectors, a new technique is developed and used in which the sensitivity vectors are obtained from the images of a specular sphere that is being illuminated from different directions. Shape and 3-D acoustically induced displacement components of cadaveric human TMs at several excitation frequencies are measured at more than one million points on its surface. A numerical rotation matrix is used to rotate the original Euclidean coordinate of the measuring system in order to obtain in-plane and out-of-plane motion components. Results show that in-plane components of motion are much smaller (<20%) than the out-of-plane motions' components.

  4. Three-dimensional vibrometry of the human eardrum with stroboscopic lensless digital holography

    PubMed Central

    Khaleghi, Morteza; Furlong, Cosme; Ravicz, Mike; Cheng, Jeffrey Tao; Rosowski, John J.

    2015-01-01

    Abstract. The eardrum or tympanic membrane (TM) transforms acoustic energy at the ear canal into mechanical motions of the ossicles. The acousto-mechanical transformer behavior of the TM is determined by its shape, three-dimensional (3-D) motion, and mechanical properties. We have developed an optoelectronic holographic system to measure the shape and 3-D sound-induced displacements of the TM. The shape of the TM is measured with dual-wavelength holographic contouring using a tunable near IR laser source with a central wavelength of 780 nm. 3-D components of sound-induced displacements of the TM are measured with the method of multiple sensitivity vectors using stroboscopic holographic interferometry. To accurately obtain sensitivity vectors, a new technique is developed and used in which the sensitivity vectors are obtained from the images of a specular sphere that is being illuminated from different directions. Shape and 3-D acoustically induced displacement components of cadaveric human TMs at several excitation frequencies are measured at more than one million points on its surface. A numerical rotation matrix is used to rotate the original Euclidean coordinate of the measuring system in order to obtain in-plane and out-of-plane motion components. Results show that in-plane components of motion are much smaller (<20%) than the out-of-plane motions’ components. PMID:25652791

  5. Virtual shearing interferometry by digital holography

    NASA Astrophysics Data System (ADS)

    Cai, L. Z.; Meng, X. F.; Wang, Y. R.; Shen, X. X.; Dong, G. Y.; Yang, X. L.

    2006-03-01

    A novel method of virtual shearing interferometry (VSI) is proposed. In this method, the shearogram is obtained by interference of a real object wave-front and a virtual object wave-front. The former is optically recorded and then digitally reconstructed; and the latter is introduced digitally by repositioning or reforming the former. The obvious advantages of VSI over conventional shearing interferometry (SI) are its versatility, accuracy, and simplicity. Only one real field is necessary to produce shearogram; there is no need of any real shearing device or even the phase unwrapping computation; and the digital shear can take any possible form according to different purposes. Both the optical experiments and computer simulations with lateral shearing, 180° rotational shearing and double lateral shearing for evaluation of lens aberrations in the general case including spherical aberration, coma, astigmatism, defocus, and tilts based on phase-shifting interferometry are given to verify the effectiveness of this method.

  6. Shedding light on diatom photonics by means of digital holography.

    PubMed

    Di Caprio, Giuseppe; Coppola, Giuseppe; De Stefano, Luca; De Stefano, Mario; Antonucci, Alessandra; Congestri, Roberta; De Tommasi, Edoardo

    2014-05-01

    Diatoms are among the dominant phytoplankters in the world's oceans, and their external silica investments, resembling artificial photonic crystals, are expected to play an active role in light manipulation. Digital holography allowed studying the interaction with light of Coscinodiscus wailesii cell wall reconstructing the light confinement inside the cell cytoplasm, condition that is hardly accessible via standard microscopy. The full characterization of the propagated beam, in terms of quantitative phase and intensity, removed a long-standing ambiguity about the origin of the light confinement. The data were discussed in the light of living cell behavior in response to their environment.

  7. Analysis of clarinet reed oscillations with digital Fresnel holography

    NASA Astrophysics Data System (ADS)

    Picart, P.; Leval, J.; Piquet, F.; Boileau, J.-P.; Dalmont, J.-P.

    2009-07-01

    This paper describes optical instrumentation devoted to vibration analysis. Two strategies based on digital Fresnel holography are presented. The first, called time-averaging consists in the numerical reconstruction of the hologram after recording with an exposure time much greater than the vibration period. Thus, the holograms are amplitude modulated by a Bessel function. The last strategy, using a pulsed regime, allows the reconstruction of the full movement of the vibration, even if it exhibits very high amplitude. Experimental results presented in this paper concern the study of the vibrations of a clarinet reed under free and forced oscillation regimes.

  8. Parallel phase-shifting digital holography using spectral estimation technique.

    PubMed

    Xia, Peng; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Matoba, Osamu

    2014-09-20

    We propose a parallel phase-shifting digital holography using a spectral estimation technique, which enables the instantaneous acquisition of spectral information and three-dimensional (3D) information of a moving object. In this technique, an interference fringe image that contains six holograms with two phase shifts for three laser lines, such as red, green, and blue, is recorded by a space-division multiplexing method with single-shot exposure. The 3D monochrome images of these three laser lines are numerically reconstructed by a computer and used to estimate the spectral reflectance distribution of object using a spectral estimation technique. Preliminary experiments demonstrate the validity of the proposed technique.

  9. Full-field vibrometry with digital Fresnel holography

    SciTech Connect

    Leval, Julien; Picart, Pascal; Boileau, Jean Pierre; Pascal, Jean Claude

    2005-09-20

    A setup that permits full-field vibration amplitude and phase retrieval with digital Fresnel holography is presented. Full reconstruction of the vibration is achieved with a three-step stroboscopic holographic recording, and an extraction algorithm is proposed. The finite temporal width of the illuminating light is considered in an investigation of the distortion of the measured amplitude and phase. In particular, a theoretical analysis is proposed and compared with numerical simulations that show good agreement. Experimental results are presented for a loudspeaker under sinusoidal excitation; the mean quadratic velocity extracted from amplitude evaluation under two different measuring conditions is presented. Comparison with time averaging validates the full-field vibrometer.

  10. Digital in-line holography for biological applications

    PubMed Central

    Xu, Wenbo; Jericho, M. H.; Meinertzhagen, I. A.; Kreuzer, H. J.

    2001-01-01

    Digital in-line holography with numerical reconstruction has been developed into a new tool, specifically for biological applications, that routinely achieves both lateral and depth resolution, at least at the micron level, in three-dimensional imaging. The experimental and numerical procedures have been incorporated into a program package with a very fast reconstruction algorithm that is now capable of real-time reconstruction. This capability is demonstrated for diverse objects, such as suspension of microspheres and biological samples (diatom, the head of Drosophila melanogaster), and the advantages are discussed by comparing holographic reconstructions with images taken by using conventional compound light microscopy. PMID:11572982

  11. Applications of short-coherence digital holography in microscopy.

    PubMed

    Martínez-León, Lluís; Pedrini, Giancarlo; Osten, Wolfgang

    2005-07-01

    We present an optical system based on short-coherence digital holography suitable for the imaging of three-dimensional microscopic objects. The short temporal coherence properties of the light source allow optical sectioning of the sample. Proper reconstruction of different layers within biological samples is possible up to a depth of a few hundred micrometers, but multiple scattering and inhomogeneities in the refractive index reduce the imaging quality for deeper layers. We have studied the possibility of numerically correcting sample-induced aberrations, and we now propose a method of improving image quality. Numerical simulations and preliminary experimental results show that compensation of these aberrations is possible to some extent.

  12. Detection of surface strain by three-dimensional digital holography

    NASA Astrophysics Data System (ADS)

    de La Torre-Ibarra, Manuel; Mendoza-Santoyo, Fernando; Pérez-López, Carlos; Saucedo-A., Tonatiuh

    2005-01-01

    Three-dimensional digital holography with three object-illuminating beams has been successfully used for the detection of surface strain in metallic objects. The optical setup that uses illuminating beams to irradiate the object from three directions means that all three object surface displacement components, x, y, and z, can be independently calculated and used to find the strain gradients on the surface. The results show the conversion of the complete surface displacement field into a surface strain field. The method is capable of measuring microstrains for out-of-plane surface displacements of less than 10 μm.

  13. Single-shot phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Zhang, Jing; Xie, Yu; Li, Guifang; Ye, Yutang; Saleh, Bahaa E. A.

    2013-03-01

    Single-shot three-dimensional biological imaging has broad applications in basic and clinical research as well as clinical use. We propose a three-dimensional imaging system that can be configured in transmission, reflection or fluorescence modes. This system is capable of recording/measuring the amplitude, phase and polarization of an optical wavefront in a single shot and reconstructing numerically objects with three-dimensional volumetric information. Built upon the principle of digital holography (DH), the proposed system uses a reference beam to interfere with the light field under investigation and digitally records/measures the in-phase and quadrature interference patterns. Single-shot in-phase and quadrature interference pattern recording is made possible by incorporating technologies widely used in coherent optical communication. Specifically, a free-space optical 90° hybrid is employed to measure the complex (real and imaginary) optical field from transmission through, reflection from or fluorescence of biological samples. We have constructed a single-shot phase-shifting digital holography system and experimentally demonstrated its operation for the first time.

  14. Miniaturized digital holography sensor for distal three-dimensional endoscopy.

    PubMed

    Kolenovic, Ervin; Osten, Wolfgang; Klattenhoff, Reiner; Lai, Songcan; von Kopylow, Christoph; Jüptner, Werner

    2003-09-01

    A miniaturized sensor head for endoscopic measurements based on digital holography is described. The system was developed to measure the shape and the three-dimensional deformation of objects located at places to which there is no access by common measurement systems. A miniaturized optical sensor, including a complete digital holographic interferometer with a CCD camera, is placed at the end of a flexible endoscope. The diameter of the head is smaller than 10 mm. The system enables interferometric measurements to be made at speeds of as many as five reconstructions per second, and it can be used outside the laboratory under normal environmental conditions. Shape measurements are performed with two wavelengths for contouring, and the deformation is measured by digital holographic interferometry. To obtain full three-dimensional data in displacement measurements we illuminate the object sequentially from three different illumination directions. To increase the lateral resolution we use temporal phase shifting.

  15. Three-dimensional information encryption and anticounterfeiting using digital holography.

    PubMed

    Shiu, Min-Tzung; Chew, Yang-Kun; Chan, Huang-Tian; Wong, Xin-Yu; Chang, Chi-Ching

    2015-01-01

    In this work, arbitrary micro phase-step digital holography with optical interferometry and digital image processing is utilized to obtain information about an image of a three-dimensional object and encrypting keys. Then, a computer-generated hologram is used for the purpose of holographic encryption. All information about the keys is required to perform the decryption, comprising the amplitude and phase distribution of the encrypting key, the distance of image reconstruction, zero-order term elimination, and twin-image term suppression. In addition to using identifiable information on different image planes and linear superposition processing hidden within the encrypted information, not only can we convey an important message, but we can also achieve anticounterfeiting. This approach retains the strictness of traditional holographic encryption and the convenience of digital holographic processing without image distortion. Therefore, this method provides better solutions to earlier methods for the security of the transmission of holographic information.

  16. Insect Wing Displacement Measurement Using Digital Holography

    SciTech Connect

    Aguayo, Daniel D.; Mendoza Santoyo, Fernando; Torre I, Manuel H. de la; Caloca Mendez, Cristian I.

    2008-04-15

    Insects in flight have been studied with optical non destructive techniques with the purpose of using meaningful results in aerodynamics. With the availability of high resolution and large dynamic range CCD sensors the so called interferometric digital holographic technique was used to measure the surface displacement of in flight insect wings, such as butterflies. The wings were illuminated with a continuous wave Verdi laser at 532 nm, and observed with a CCD Pixelfly camera that acquire images at a rate of 11.5 frames per second at a resolution of 1392x1024 pixels and 12 Bit dynamic range. At this frame rate digital holograms of the wings were captured and processed in the usual manner, namely, each individual hologram is Fourier processed in order to find the amplitude and phase corresponding to the digital hologram. The wings displacement is obtained when subtraction between two digital holograms is performed for two different wings position, a feature applied to all consecutive frames recorded. The result of subtracting is seen as a wrapped phase fringe pattern directly related to the wing displacement. The experimental data for different butterfly flying conditions and exposure times are shown as wire mesh plots in a movie of the wings displacement.

  17. High-resolution synthetic-aperture digital holography with digital phase and pupil correction.

    PubMed

    Tippie, Abbie E; Kumar, Abhishek; Fienup, James R

    2011-06-20

    A 218 mega-pixel synthetic aperture was collected by raster scanning a CCD detector in a digital holography imaging experiment. Frames were mosaicked together using a two-step cross-correlation registration. Phase correction using sharpness metrics were utilized to achieve diffraction-limited resolution.

  18. Single shot high resolution digital holography.

    PubMed

    Khare, Kedar; Ali, P T Samsheer; Joseph, Joby

    2013-02-11

    We demonstrate a novel computational method for high resolution image recovery from a single digital hologram frame. The complex object field is obtained from the recorded hologram by solving a constrained optimization problem. This approach which is unlike the physical hologram replay process is shown to provide high quality image recovery even when the dc and the cross terms in the hologram overlap in the Fourier domain. Experimental results are shown for a Fresnel zone hologram of a resolution chart, intentionally recorded with a small off-axis reference beam angle. Excellent image recovery is observed without the presence of dc or twin image terms and with minimal speckle noise.

  19. Lensless Imaging for Battlefield On-Chip Blood Diagnostics

    DTIC Science & Technology

    2010-12-06

    University of Southern California (USC), 28 April 2010, Los Angeles CA 15. A. Ozcan, “Incoherent Lensfree Cell Holography for Global Health...Multi-angle Lensless Holography for Depth Resolved High-throughput Imaging of Cells On a Chip,” IEEE Photonics Society, Winter Topical Meeting on...and A. Ozcan, “Incoherent On-chip Cell Holography for Sub-cellular Imaging and Point-of-Care Diagnostics,” SPIE Photonics West Conference, Imaging

  20. Pixel super-resolution in digital in-line holography

    NASA Astrophysics Data System (ADS)

    Wang, Mingjun; Feng, Shaodong; Wu, Jigang

    2016-10-01

    We report a new holographic microscope using pixel super-resolution algorithm. In our method, a sequence of low resolution images are acquired by a complementary metal oxide semiconductor (CMOS) sensor in digital inline holography system and the resolution is limited by the sensor pixel size. Then the super-resolution algorithm is applied to the low resolution images to get the image with much higher resolution that beyond the Nyquist criteria. We perform both numerical simulation and experiments to demonstrate our method with US Air Force Target used as the sample. The sample is randomly moved in the sample plane and a set of holograms are captured by the camera in inline holographic system. We use two methods to reconstruct the sample image. In the first method, super-resolution algorithm is applied with the low resolution holograms to get the high resolution hologram. Then the high resolution hologram is reconstructed using auto-focusing algorithm to get the high resolution sample image. In the second method, the raw holograms are directly reconstructed to get a set of low resolution sample images, then the super-resolution algorithm is applied to get the high resolution sample image. We observed that the above mentioned two methods can get similar results in both numerical stimulation and experiments. We believe that the combination of pixel super-resolution algorithm and digital in-line holography can be very useful to implement a compact low-cost microscope with high resolution.

  1. Optical voice recorder by off-axis digital holography.

    PubMed

    Matoba, Osamu; Inokuchi, Hiroki; Nitta, Kouichi; Awatsuji, Yasuhiro

    2014-11-15

    An optical voice recorder capable of recording and reproducing propagating sound waves by using off-axis digital holography, as well as quantitative visualization, is presented. Propagating sound waves temporally modulate the phase distribution of an impinging light wave via refractive index changes. This temporally modulated phase distribution is recorded in the form of digital holograms by a high-speed image sensor. After inverse propagation using Fresnel diffraction of a series of the recorded holograms, the temporal phase profile of the reconstructed object wave at each three-dimensional position can be used to reproduce the original sound wave. Experimental results using a tuning fork vibrating at 440 Hz and a human voice are presented to show the feasibility of the proposed method.

  2. Femtosecond digital holography based on spatial light modulator

    NASA Astrophysics Data System (ADS)

    Lu, Xiaowei; Li, Jingzhen; Chen, Hongyi

    2013-12-01

    A high-speed digital holography based on a liquid crystal Spatial Light Modulator (LC-SLM) is presented. The production of multi-beam and control of their delay time can be achieved by controllable wavefront coding theory. In this paper, a Dammann phase grating was designed using a SLM to product multi-beam for extremely high speed digital holographic imaging. The required beam deflection could be obtained by adjusting programmable phase mask for controlling the spatial diffraction of input beam. The framing time is decided by interval beam angles and the distances between input plane and record plane. Theoretical derivate of generation multi beams based on SLM was proposed, and phase recover software was also made.

  3. Effects of quantization in phase-shifting digital holography.

    PubMed

    Mills, Godfrey A; Yamaguchi, Ichirou

    2005-03-01

    We discuss quantization effects of hologram recording on the quality of reconstructed images in phase-shifting digital holography. We vary bit depths of phase-shifted holograms in both numerical simulation and experiments and then derived the complex amplitude, which is subjected to Fresnel transformation for the image reconstruction. The influence of bit-depth limitation in quantization has been demonstrated in a numerical simulation for spot-array patterns with linearly varying intensities and a continuous intensity object. The objects are provided with uniform and random phase modulation. In experiments, digital holograms are originally recorded at 8 bits and the bit depths are changed to deliver holograms at bit depths of 1 to 8 bits for the image reconstruction. The quality of the reconstructed images has been evaluated for the different quantization levels.

  4. Fast phase retrieval in slightly off-axis digital holography

    NASA Astrophysics Data System (ADS)

    Zhong, Zhi; Bai, Hongyi; Shan, Mingguang; Zhang, Yabin; Guo, Lili

    2017-10-01

    In this study, three efficient algorithms are proposed for fast phase retrieval in slightly off-axis digital holography using spectrum cropping, spatial multiplexing, and complex encoding. In the first algorithm, the real spectral order of the subtracted hologram is filtered and cropped, and the number of pixels is decreased in the subsequent retrieval operations. In the second algorithm, two sequential subtracted holograms are digitally phase shifted and spatial multiplexed into one synthetic hologram, and thus only one inverse Fourier transformation is then required. In the third algorithm, two sequential subtracted holograms are encoded separately into the real part and the imaginary part of a complex hologram. Two cross-correlations can be used to reconstruct the phase, thereby improving the utilization of the spectrum. The three new algorithms speed up our previously proposed retrieval method with the assistance of specimen-free holograms. Our experiments demonstrated the validity and improved time requirements of the proposed methods.

  5. Fast autofocusing in digital holography using the magnitude differential.

    PubMed

    Lyu, Meng; Yuan, Caojin; Li, Dayan; Situ, Guohai

    2017-05-01

    Typical methods of automatic estimation of focusing in digital holography calculate every single reconstructed frame to get a critical function and then ascertain the focal plane by finding the extreme value of that function. Here, we propose a digital holographic autofocusing method that computes the focused distance using the first longitudinal difference of the magnitude of the reconstructed image. We demonstrate the proposed method with both numerical simulations and optical experiments of amplitude-contrast and phase-contrast objects. The results suggest that the proposed method performs better than other existing methods, in terms of applicability and computation efficiency, with potential applications in industrial and biomedical inspections where automatic focus tracking is necessary.

  6. Parallel phase-shifting digital holography system using a high-speed camera

    NASA Astrophysics Data System (ADS)

    Awatsuji, Yasuhiro; Kakue, Takashi; Tahara, Tatsuki; Xia, Peng; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2012-11-01

    A technique of single-shot phase-shifting interferometry called as parallel phase-shifting digital holography is described. This technique records multiple holograms required for phase-shifting digital holography using space-division multiplexing technique. The authors constructed a system based on the parallel phase-shifting digital holography consisting of a Mach-Zehnder interferometer and a high-speed polarization imaging camera. High-speed motion pictures of three-dimensional image and phase image of dynamically moving objects at the rate up to180,000 and 262,500 frames per second were achieved, respectively, for the 128 × 128-pixel images.

  7. Underwater digital holography for studies of marine plankton.

    PubMed

    Sun, H; Benzie, P W; Burns, N; Hendry, D C; Player, M A; Watson, J

    2008-05-28

    Conventional and digital holographies are proving to be increasingly important for studies of marine zooplankton and other underwater biological applications. This paper reports on the use of a subsea digital holographic camera (eHoloCam) for the analysis and identification of marine organisms and other subsea particles. Unlike recording on a photographic film, a digital hologram (e-hologram) is recorded on an electronic sensor and reconstructed numerically in a computer by simulating the propagation of the optical field in space. By comparison with other imaging techniques, an e-hologram has several advantages such as three-dimensional spatial reconstruction, non-intrusive and non-destructive interrogation of the recording sampling volume and the ability to record holographic videos. The basis of much work in optics lies in Maxwell's electromagnetic theory and holography is no exception: we report here on two of the numerical reconstruction algorithms we have used to reconstruct holograms obtained using eHoloCam and how their starting point lies in Maxwell's equations. Derivation of the angular spectrum algorithm for plane waves is provided as an exact method for the in-line numerical reconstruction of digital holograms. The Fresnel numerical reconstruction algorithm is derived from the angular spectrum method. In-line holograms are numerically processed before and after reconstruction to remove periodic noise from captured images and to increase image contrast. The ability of the Fresnel integration reconstruction algorithm to extend the reconstructed volume beyond the recording sensor dimensions is also shown with a 50% extension of the reconstruction area. Finally, we present some images obtained from recent deployments of eHoloCam in the North Sea and Faeroes Channel.

  8. Digital polarization holography advancing geometrical phase optics.

    PubMed

    De Sio, Luciano; Roberts, David E; Liao, Zhi; Nersisyan, Sarik; Uskova, Olena; Wickboldt, Lloyd; Tabiryan, Nelson; Steeves, Diane M; Kimball, Brian R

    2016-08-08

    Geometrical phase or the fourth generation (4G) optics enables realization of optical components (lenses, prisms, gratings, spiral phase plates, etc.) by patterning the optical axis orientation in the plane of thin anisotropic films. Such components exhibit near 100% diffraction efficiency over a broadband of wavelengths. The films are obtained by coating liquid crystalline (LC) materials over substrates with patterned alignment conditions. Photo-anisotropic materials are used for producing desired alignment conditions at the substrate surface. We present and discuss here an opportunity of producing the widest variety of "free-form" 4G optical components with arbitrary spatial patterns of the optical anisotropy axis orientation with the aid of a digital spatial light polarization converter (DSLPC). The DSLPC is based on a reflective, high resolution spatial light modulator (SLM) combined with an "ad hoc" optical setup. The most attractive feature of the use of a DSLPC for photoalignment of nanometer thin photo-anisotropic coatings is that the orientation of the alignment layer, and therefore of the fabricated LC or LC polymer (LCP) components can be specified on a pixel-by-pixel basis with high spatial resolution. By varying the optical magnification or de-magnification the spatial resolution of the photoaligned layer can be adjusted to an optimum for each application. With a simple "click" it is possible to record different optical components as well as arbitrary patterns ranging from lenses to invisible labels and other transparent labels that reveal different images depending on the side from which they are viewed.

  9. Three-dimensional-object recognition by use of single-exposure on-axis digital holography.

    PubMed

    Javidi, Bahram; Kim, Daesuk

    2005-02-01

    On-axis phase-shifting digital holography requires recording of multiple holograms. We describe a novel real-time three-dimensional- (3-D-) object recognition system that uses single-exposure on-axis digital holography. In contrast to 3-D-object recognition by means of a conventional phase-shifting scheme that requires multiple exposures, our proposed method requires only a single digital hologram to be synthesized and used to recognize 3-D objects. A benefit of the proposed 3-D recognition method is enhanced practicality of digital holography for 3-D recognition in terms of its simplicity and greater robustness to external scene parameters such as moving targets and environmental noise factors. We show experimentally the utility of the single-exposure on-axis digital holography-based 3-D-object recognition method.

  10. Joint Applied Optics and Chinese Optics Letters feature introduction: digital holography and three-dimensional imaging.

    PubMed

    Poon, Ting-Chung

    2011-12-01

    This feature issue serves as a pilot issue promoting the joint issue of Applied Optics and Chinese Optics Letters. It focuses upon topics of current relevance to the community working in the area of digital holography and 3-D imaging.

  11. The application of digital image plane holography technology to identify Chinese herbal medicine

    NASA Astrophysics Data System (ADS)

    Wang, Huaying; Guo, Zhongjia; Liao, Wei; Zhang, Zhihui

    2012-03-01

    In this paper, the imaging technology of digital image plane holography to identify the Chinese herbal medicine is studied. The optical experiment system of digital image plane holography which is the special case of pre-magnification digital holography was built. In the record system, one is an object light by using plane waves which illuminates the object, and the other one is recording hologram by using spherical light wave as reference light. There is a Micro objective lens behind the object. The second phase factor which caus ed by the Micro objective lens can be eliminated by choosing the proper position of the reference point source when digital image plane holography is recorded by spherical light. In this experiment, we use the Lygodium cells and Onion cells as the object. The experiment results with Lygodium cells and Onion cells show that digital image plane holography avoid the process of finding recording distance by using auto-focusing approach, and the phase information of the object can be reconstructed more accurately. The digital image plane holography is applied to the microscopic imaging of cells more effectively, and it is suit to apply for the identify of Chinese Herbal Medicine. And it promotes the application of digital holographic in practice.

  12. The application of digital image plane holography technology to identify Chinese herbal medicine

    NASA Astrophysics Data System (ADS)

    Wang, Huaying; Guo, Zhongjia; Liao, Wei; Zhang, Zhihui

    2011-11-01

    In this paper, the imaging technology of digital image plane holography to identify the Chinese herbal medicine is studied. The optical experiment system of digital image plane holography which is the special case of pre-magnification digital holography was built. In the record system, one is an object light by using plane waves which illuminates the object, and the other one is recording hologram by using spherical light wave as reference light. There is a Micro objective lens behind the object. The second phase factor which caus ed by the Micro objective lens can be eliminated by choosing the proper position of the reference point source when digital image plane holography is recorded by spherical light. In this experiment, we use the Lygodium cells and Onion cells as the object. The experiment results with Lygodium cells and Onion cells show that digital image plane holography avoid the process of finding recording distance by using auto-focusing approach, and the phase information of the object can be reconstructed more accurately. The digital image plane holography is applied to the microscopic imaging of cells more effectively, and it is suit to apply for the identify of Chinese Herbal Medicine. And it promotes the application of digital holographic in practice.

  13. Off-axis illumination direct-to-digital holography

    DOEpatents

    Thomas, Clarence E.; Price, Jeffery R.; Voelkl, Edgar; Hanson, Gregory R.

    2004-06-08

    Systems and methods are described for off-axis illumination direct-to-digital holography. A method of recording an off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis, includes: reflecting a reference beam from a reference mirror at a non-normal angle; reflecting an object beam from an object at an angle with respect to an optical axis defined by a focusing lens; focusing the reference beam and the object beam at a focal plane of a digital recorder to form the off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis; digitally recording the off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis; Fourier analyzing the recorded off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes by transforming axes of the recorded off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes in Fourier space to sit on top of a heterodyne carrier frequency defined as an angle between the reference beam and the object beam; applying a digital filter to cut off signals around an original origin; and then performing an inverse Fourier transform.

  14. Low photon count based digital holography for quadratic phase cryptography.

    PubMed

    Muniraj, Inbarasan; Guo, Changliang; Malallah, Ra'ed; Ryle, James P; Healy, John J; Lee, Byung-Geun; Sheridan, John T

    2017-07-15

    Recently, the vulnerability of the linear canonical transform-based double random phase encryption system to attack has been demonstrated. To alleviate this, we present for the first time, to the best of our knowledge, a method for securing a two-dimensional scene using a quadratic phase encoding system operating in the photon-counted imaging (PCI) regime. Position-phase-shifting digital holography is applied to record the photon-limited encrypted complex samples. The reconstruction of the complex wavefront involves four sparse (undersampled) dataset intensity measurements (interferograms) at two different positions. Computer simulations validate that the photon-limited sparse-encrypted data has adequate information to authenticate the original data set. Finally, security analysis, employing iterative phase retrieval attacks, has been performed.

  15. Adaptive flow-field measurements using digital holography

    NASA Astrophysics Data System (ADS)

    Czarske, Jürgen W.; Koukourakis, Nektarios; Fregin, Bob; König, Jörg; Büttner, Lars

    2017-02-01

    Variations of the optical detection path-length in image correlation based flow-field measurements result in strong errors in position allocation and thus lead to a strong enhancement of the measurement uncertainty of the velocity. In this contribution we use digital holography to measure the wavefront distortion induced by fluctuating phase boundary, employing spatially extended guide stars. The measured phase information is used to correct the influence of the phase boundary in the detection path employing a spatial light modulator. We analyze the potential of guide stars that are reflected by the phase boundary, i.e. the Fresnel reflex, and transmitted. Our results show, that the usage of wavefront shaping enables to strongly reduce the measurement uncertainty and to strongly improve the quality of image correlation based flow-field measurements. The approaches presented here are not limited to application in flow measurement, but could be useful for a variety of applications.

  16. Time-resolved image plane off-axis digital holography

    NASA Astrophysics Data System (ADS)

    Petrov, N. V.; Putilin, S. E.; Chipegin, A. A.

    2017-04-01

    In this work, we demonstrate off-axis image-plane digital holography for measuring ultrafast processes with high temporal resolution. The proposed image-plane holographic configuration in conjunction with numerical post-processing procedures allows us to neglect the walk-off effect in the off-axis arrangement by synthesizing spatial phase distribution with the whole field of view from separate fragments and to increase the spatial resolution by means of a telecentric system with adjustable magnification. We have analyzed temporal resolution taking into account all dispersing elements that increase the duration of the pulses being propagated through the optical setup. The technique was approved with experiment on measuring the dynamics of the refractive index, induced by laser filamentation in air.

  17. Compressive self-interference Fresnel digital holography with faithful reconstruction

    NASA Astrophysics Data System (ADS)

    Wan, Yuhong; Man, Tianlong; Han, Ying; Zhou, Hongqiang; Wang, Dayong

    2017-05-01

    We developed compressive self-interference digital holographic approach that allows retrieving three-dimensional information of the spatially incoherent objects from single-shot captured hologram. The Fresnel incoherent correlation holography is combined with parallel phase-shifting technique to instantaneously obtain spatial-multiplexed phase-shifting holograms. The recording scheme is regarded as compressive forward sensing model, thus the compressive-sensing-based reconstruction algorithm is implemented to reconstruct the original object from the under sampled demultiplexed sub-holograms. The concept was verified by simulations and experiments with simulating use of the polarizer array. The proposed technique has great potential to be applied in 3D tracking of spatially incoherent samples.

  18. Cell imaging techniques based on digital image plane holography

    NASA Astrophysics Data System (ADS)

    Chen, Zhaoji; Gong, Wendi; Liu, Feifei; Wang, Huaying

    2010-11-01

    This paper has further studied the implementation methods and recording conditions of digital microscopic image plane holography (DMIPH). Two optical systems of DMIPH were built: one is recording hologram by using plane waves as reference light, the other is recording hologram by spherical reference light. Breast cancer cells and USAF resolution test target is used as tested samples in the experiment. Then the intensity distribution and three-dimensional shape information of the cells are got accurately. The experiment results show that DMIPH avoids the process of finding recording distance by using auto-focusing approach. The recording and reconstruction process of DMIPH is simple. Therefore DMIPH can be applied to the microscopic imaging of cells more effectively.

  19. Object recognition by use of polarimetric phase-shifting digital holography.

    PubMed

    Nomura, Takanori; Javidi, Bahram

    2007-08-01

    Pattern recognition by use of polarimetric phase-shifting digital holography is presented. Using holography, the amplitude distribution and phase difference distribution between two orthogonal polarizations of three-dimensional (3D) or two-dimensional phase objects are obtained. This information contains both complex amplitude and polarimetric characteristics of the object, and it can be used for improving the discrimination capability of object recognition. Experimental results are presented to demonstrate the idea. To the best of our knowledge, this is the first report on 3D polarimetric recognition of objects using digital holography.

  20. Analysis of common-path incoherent digital holography using dual-focusing lens with diffraction gratings

    NASA Astrophysics Data System (ADS)

    Quan, Xiangyu; Moriyama, Asuka; Kouichi, Nitta; Matoba, Osamu; Awatsuji, Yasuhiro

    2017-04-01

    This paper provides overall analytical insights on the common-path incoherent digital holography using dual-focusing lens with diffraction gratings. Especially when one tries to seek an off-axis solution using the suggested configuration, the low temporal and spatial coherence require specific conditions on parameters of the set-up. AA mathematical explanation on the off-axis digital holography is described.

  1. Parallel phase-shifting digital holography with adaptive function using phase-mode spatial light modulator.

    PubMed

    Lin, Miao; Nitta, Kouichi; Matoba, Osamu; Awatsuji, Yasuhiro

    2012-05-10

    Parallel phase-shifting digital holography using a phase-mode spatial light modulator (SLM) is proposed. The phase-mode SLM implements spatial distribution of phase retardation required in the parallel phase-shifting digital holography. This SLM can also compensate dynamically the phase distortion caused by optical elements such as beam splitters, lenses, and air fluctuation. Experimental demonstration using a static object is presented.

  2. Image quality improvement of parallel four-step phase-shifting digital holography by using the algorithm of parallel two-step phase-shifting digital holography.

    PubMed

    Kakue, Takashi; Moritani, Yuri; Ito, Kenichi; Shimozato, Yuki; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2010-04-26

    We propose an algorithm that can improve the quality of the reconstructed image from the single hologram recorded by the optical system of the parallel four-step phase-shifting digital holography. The proposed algorithm applies the image-reconstruction algorithm of parallel two-step phase-shifting digital holography to the hologram so as to reduce errors in the reconstructed image and eliminate ghosts. We numerically and experimentally confirmed that the proposed algorithm decreased 25% in terms of root mean square error in amplitude, and eliminated the ghosts, respectively.

  3. Optical encryption of binary data information with 2-step phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Gil, Sang Keun; Byun, Hyun Joong; Lee, Hyun Jin; Jeon, Seok Hee; Jeong, Jong Rae

    2007-02-01

    We propose an optical encryption/decryption technique based on 2-step phase-shifting digital holography for a cipher system. The technique using 2-step phase-shifting digital holography is more efficient than 4-step phase-shifting digital holography because the 2-step method has less data than 4-step method to restore or transmit the encrypted data. In our system, 2-step phase-shifting digital holograms are acquired by moving the PZT mirror with phase step of 0 or π/2 in the reference beam path and are recorded on CCD camera. The information data and the key are expressed with random binary amplitude and random phase. Digital hologram in this method is Fourier transform hologram and digitized with 256 gray-level. DC-term removal is essential to reconstruct and decrypt the original binary data information. The simulation shows that the proposed method gives good results for cipher system. The quantization error is also analyzed.

  4. Distortion-tolerant 3-D object recognition by using single exposure on-axis digital holography.

    PubMed

    Kim, Daesuk; Javidi, Bahram

    2004-11-01

    We present a distortion-tolerant 3-D object recognition system using single exposure on-axis digital holography. In contrast to distortion-tolerant 3-D object recognition employing conventional phase shifting scheme which requires multiple exposures, our proposed method requires only one single digital hologram to be synthesized and used for distortion-tolerant 3-D object recognition. A benefit of the single exposure based on-axis approach is enhanced practicality of digital holography for distortion-tolerant 3-D object recognition in terms of its simplicity and high tolerance to external scene parameters such as moving targets. This paper shows experimentally, that single exposure on-axis digital holography is capable of providing a distortion-tolerant 3-D object recognition capability.

  5. Applications of Digital Holography: From Microscopy to 3D-Television

    NASA Astrophysics Data System (ADS)

    Kreis, T.

    2012-03-01

    The paper gives an overview of the applications of digital holography based on the one hand on CCD-recording, computer storage, and numerical reconstruction of the wave fields, and on the other hand on numerical calculation of computer generated holograms (CGH) and the transfer of these CGHs to spatial light modulators (SLM) for optical reconstruction of the wave fields. The first mentioned type of digital holography finds applications in digital holographic microscopy, particle analysis, and interferometric form and deformation measurement, while the second type constitutes the basis for holographic 3D TV. The space-bandwidth-problem occuring in this context is addressed and first partial solutions are presented.

  6. Digital reflection holography based systems development for MEMS testing

    NASA Astrophysics Data System (ADS)

    Singh, Vijay Raj; Liansheng, Sui; Asundi, Anand

    2010-05-01

    MEMS are tiny mechanical devices that are built onto semiconductor chips and are measured in micrometers and nanometers. Testing of MEMS device is an important part in carrying out their functional assessment and reliability analysis. Development of systems based on digital holography (DH) for MEMS inspection and characterization is presented in this paper. Two DH reflection systems, table-top and handheld types, are developed depending on the MEMS measurement requirements and their capabilities are presented. The methodologies for the systems are developed for 3D profile inspection and static & dynamic measurements, which is further integrated with in-house developed software that provides the measurement results in near real time. The applications of the developed systems are demonstrated for different MEMS devices for 3D profile inspection, static deformation/deflection measurements and vibration analysis. The developed systems are well suitable for the testing of MEMS and Microsystems samples, with full-field, static & dynamic inspection as well as to monitor micro-fabrication process.

  7. Exact complex-wave reconstruction in digital holography.

    PubMed

    Seelamantula, Chandra Sekhar; Pavillon, Nicolas; Depeursinge, Christian; Unser, Michael

    2011-06-01

    We address the problem of exact complex-wave reconstruction in digital holography. We show that, by confining the object-wave modulation to one quadrant of the frequency domain, and by maintaining a reference-wave intensity higher than that of the object, one can achieve exact complex-wave reconstruction in the absence of noise. A feature of the proposed technique is that the zero-order artifact, which is commonly encountered in hologram reconstruction, can be completely suppressed in the absence of noise. The technique is noniterative and nonlinear. We also establish a connection between the reconstruction technique and homomorphic signal processing, which enables an interpretation of the technique from the perspective of deconvolution. Another key contribution of this paper is a direct link between the reconstruction technique and the two-dimensional Hilbert transform formalism proposed by Hahn. We show that this connection leads to explicit Hilbert transform relations between the magnitude and phase of the complex wave encoded in the hologram. We also provide results on simulated as well as experimental data to validate the accuracy of the reconstruction technique.

  8. In vitro imaging of ophthalmic tissue by digital interference holography

    NASA Astrophysics Data System (ADS)

    Potcoava, Mariana C.; Kay, Christine N.; Kim, Myung K.; Richards, David W.

    2010-01-01

    We used digital interference holography (DIH) for in vitro imaging of human optic nerve head and retina. Samples of peripheral retina, macula, and optic nerve head from two formaldehyde-preserved human eyes were dissected and mounted onto slides. Holograms were captured by a monochrome CCD camera (Sony XC-ST50, with 780 × 640 pixels and pixel size of ∼9 µm). Light source was a solid-state pumped dye laser with tunable wavelength range of 560-605 nm. Using about 50 wavelengths in this band, holograms were obtained and numerically reconstructed using custom software based on NI LabView. Tomographic images were produced by superposition of holograms. Holograms of all tissue samples were obtained with a signal-to-noise ratio of approximately 50 dB. Optic nerve head characteristics (shape, diameter, cup depth, and cup width) were quantified with a few micron resolution (4.06-4.8 µm). Multiple layers were distinguishable in cross-sectional images of the macula. To our knowledge, this is the first report of DIH use to image human macular and optic nerve tissue. DIH has the potential to become a useful tool for researchers and clinicians in the diagnosis and treatment of many ocular diseases, including glaucoma and a variety of macular diseases.

  9. Detection of biological tissue in gels using pulsed digital holography

    NASA Astrophysics Data System (ADS)

    del Socorro Hernández-Montes, Maria; Pérez-López, C.; Mendoza Santoyo, Fernando; Muñoz Guevara, Luis Manuel

    2004-03-01

    An out of plane optical sensitive configuration for pulsed digital holography was used to detect biological tissue inside solid organic materials like gels. A loud speaker and a shaker were employed to produce a mechanical wave that propagates through the gel in such a way that it generates vibrational resonant modes and transient events on the gel surface. Gel surface micro displacements were observed between the firing of two laser pulses, both for a steady resonant mode and for different times during the transient event. The biological tissue sample inserted approximately 2 cm inside the gel diffracts the original mechanical wave and changes the resonant mode pattern or the transient wave on the gel surface. This fact is used to quantitatively measure the gel surface micro displacement. Comparison of phase unwrapped patterns, with and without tissue inside the gel, allows the rapid identification of the existence of tissue inside the gel. The results for the resonant and transient conditions show that the method may be reliably used to study, compare and distinguish data from inside homogeneous and in-homogeneous solid organic materials.

  10. Creating an extended focus image of a tilted object in Fourier digital holography.

    PubMed

    Paturzo, Melania; Ferraro, Pietro

    2009-10-26

    We present a new method to numerically reconstruct images on a tilted plane by digital holography in Fourier configuration. The proposed technique is based on a quadratic deformation of spatial coordinates of the digital hologram. By this approach we demonstrate that it is possible to recover the extended focus image (EFI) of a tilted object in a single reconstruction step from the deformed hologram.

  11. Measurement of rabbit eardrum vibration through stroboscopic digital holography

    SciTech Connect

    De Greef, Daniël; Dirckx, Joris J. J.

    2014-05-27

    In this work, we present a setup for high-power single shot stroboscopic digital holography and demonstrate it in an application on rabbit eardrum vibration measurement. The setup is able to make full-field time-resolved measurements of vibrating surfaces with a precision in the nanometer range in a broad frequency range. The height displacement of the measured object is visualized over the entire surface as a function of time. Vibration magnitude and phase maps can be extracted from these data, the latter proving to be very useful to reveal phase delays across the surface. Such deviations from modal motion indicate energy losses due to internal damping, in contrast to purely elastic mechanics. This is of great interest in middle ear mechanics and finite element modelling. In our setup, short laser pulses are fired at selected instants within the surface vibration period and are recorded by a CCD camera. The timing of the pulses and the exposure of the camera are synchronized to the vibration phase by a microprocessor. The high-power frequency-doubled Nd:YAG laser produces pulses containing up to 5 mJ of energy, which is amply sufficient to record single-shot holograms. As the laser pulse length is 8 ns and the smallest time step of the trigger electronics is 1 μs, vibration measurements of frequencies up to 250 kHz are achievable through this method, provided that the maximum vibration amplitude exceeds a few nanometers. In our application, middle ear mechanics, measuring frequencies extend from 5 Hz to 20 kHz. The experimental setup will be presented, as well as results of measurements on a stretched circular rubber membrane and a rabbit's eardrum. Two of the challenges when measuring biological tissues, such as the eardrum, are low reflectivity and fast dehydration. To increase reflectivity, a coating is applied and to counteract the undesirable effects of tissue dehydration, the measurement setup and software have been optimized for speed without compromising

  12. Measurement of rabbit eardrum vibration through stroboscopic digital holography

    NASA Astrophysics Data System (ADS)

    De Greef, Daniël; Dirckx, Joris J. J.

    2014-05-01

    In this work, we present a setup for high-power single shot stroboscopic digital holography and demonstrate it in an application on rabbit eardrum vibration measurement. The setup is able to make full-field time-resolved measurements of vibrating surfaces with a precision in the nanometer range in a broad frequency range. The height displacement of the measured object is visualized over the entire surface as a function of time. Vibration magnitude and phase maps can be extracted from these data, the latter proving to be very useful to reveal phase delays across the surface. Such deviations from modal motion indicate energy losses due to internal damping, in contrast to purely elastic mechanics. This is of great interest in middle ear mechanics and finite element modelling. In our setup, short laser pulses are fired at selected instants within the surface vibration period and are recorded by a CCD camera. The timing of the pulses and the exposure of the camera are synchronized to the vibration phase by a microprocessor. The high-power frequency-doubled Nd:YAG laser produces pulses containing up to 5 mJ of energy, which is amply sufficient to record single-shot holograms. As the laser pulse length is 8 ns and the smallest time step of the trigger electronics is 1 μs, vibration measurements of frequencies up to 250 kHz are achievable through this method, provided that the maximum vibration amplitude exceeds a few nanometers. In our application, middle ear mechanics, measuring frequencies extend from 5 Hz to 20 kHz. The experimental setup will be presented, as well as results of measurements on a stretched circular rubber membrane and a rabbit's eardrum. Two of the challenges when measuring biological tissues, such as the eardrum, are low reflectivity and fast dehydration. To increase reflectivity, a coating is applied and to counteract the undesirable effects of tissue dehydration, the measurement setup and software have been optimized for speed without compromising

  13. Study of heat dissipation process from heat sink using lensless Fourier transform digital holographic interferometry.

    PubMed

    Kumar, Varun; Shakher, Chandra

    2015-02-20

    This paper presents the results of experimental investigations about the heat dissipation process of plate fin heat sink using digital holographic interferometry. Visual inspection of reconstructed phase difference maps of the air field around the heat sink with and without electric power in the load resistor provides qualitative information about the variation of temperature and the heat dissipation process. Quantitative information about the temperature distribution is obtained from the relationship between the digitally reconstructed phase difference map of ambient air and heated air. Experimental results are presented for different current and voltage in the load resistor to investigate the heat dissipation process. The effect of fin spacing on the heat dissipation performance of the heat sink is also investigated in the case of natural heat convection. From experimental data, heat transfer parameters, such as local heat flux and convective heat transfer coefficients, are also calculated.

  14. Partition calculation for zero-order and conjugate image removal in digital in-line holography.

    PubMed

    Ma, Lihong; Wang, Hui; Li, Yong; Jin, Hongzhen

    2012-01-16

    Conventional digital in-line holography requires at least two phase-shifting holograms to reconstruct an original object without zero-order and conjugate image noise. We present a novel approach in which only one in-line hologram and two intensity values (namely the object wave intensity and the reference wave intensity) are required. First, by subtracting the two intensity values the zero-order diffraction can be completely eliminated. Then, an algorithm, called partition calculation, is proposed to numerically remove the conjugate image. A preliminary experimental result is given to confirm the proposed method. The method can simplify the procedure of phase-shifting digital holography and improve the practical feasibility for digital in-line holography.

  15. Investigation of silion MEMS structures subjected to thermal loading by digital holography

    NASA Astrophysics Data System (ADS)

    Ferraro, Pietro; De Nicola, Sergio; Finizio, Andrea; Coppola, Giuseppe; Iodice, Mario; Grilli, Simonetta; Magro, Carlo; Pierattini, Giovanni

    2003-10-01

    In this paper we study silicon MEMS (Microelectromechanical systems) structures subjected to thermal loading. Digital holography has been investigated as inspection tool to evaluate the deformation induced by the thermal loading. Application of DH on structures with several different geometries and shapes, like cantilever beams, bridges and membranes is reported and result will be discussed. Dimensions of the inspected microstructures, varies in the range 1-50μm. The experimental results shown that a "bimorph-effect" induces a deformation in MEMS structures. The difficulties encountered in performing the deformation analysis by digital holography in real-time will be afforded and discussed. A method with automatic focus tracking in Digital Holography is proposed allowing inspection of MEMS, under thermal loading, in real-time.

  16. Image authentication via sparsity-based phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Chen, Wen; Chen, Xudong

    2015-03-01

    Digital holography has been widely studied in recent years, and a number of applications have been demonstrated. In this paper, we demonstrate that sparsity-based phase-shifting digital holography can be applied for image authentication. In phase-shifting digital holography, the holograms are sequentially recorded. Only small parts of each hologram are available for numerical reconstruction. It is found that nonlinear correlation algorithm can be applied to simply authenticate the reconstructed object. The results illustrate that the recovered image can be correctly verified. In the developed system, the recorded holograms are highly compressed which can facilitate data storage or transmission, and one simple authentication strategy has been established instead of applying relatively complex algorithms (such as compressive sensing) to recover the object.

  17. Scheme to improve the reconstructed image in parallel quasi-phase-shifting digital holography.

    PubMed

    Awatsuji, Yasuhiro; Sasada, Masaki; Fujii, Atsushi; Kubota, Toshihiro

    2006-02-10

    We propose a scheme to improve the reconstructed image in parallel quasi-phase-shifting digital holography. Parallel quasi-phase-shifting digital holography is a technique capable of noiseless instantaneous measurement of three-dimensional objects, and it implements four kinds of phase shifting at a time with an array of 2 x 2 phase-shifting devices located in the reference wave. In the phase-shifting calculation in the reconstruction process of the technique, the scheme assigns the 2 x 2 cell configuration for each pixel in the vertical direction and for each 1-pixel interval in the horizontal direction of the hologram recorded by the image sensor. We conduct both a numerical simulation and a preliminary experiment. The results show that the proposed scheme can improve the quality of the reconstructed image calculated by the conventional scheme of parallel quasi-phase-shifting digital holography we previously proposed, and then the effectiveness of the proposed scheme is verified.

  18. High-speed 3D imaging by parallel phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Awatsuji, Yasuhiro; Xia, Peng; Matoba, Osamu

    2015-07-01

    As a high-speed three-dimensional (3D) imaging technique, parallel phase-shifting digital holography is presented. This technique records a single hologram of an object with an image sensor having a phase-shift array device and reconstructs the instantaneous 3D image of the object with a computer. In this technique, a single hologram in which the multiple holograms required for phase-shifting digital holography are multiplexed by using space-division multiplexing technique pixel by pixel. Also, we present a high-speed parallel phase-shifting digital holography system. The system consists of an interferometer, a continuous-wave laser, and a high-speed polarization imaging camera. Motion pictures of dynamic phenomena at the rate of up to 1,000,000 frames per second have been achieved by the high-speed system.

  19. Single-shot femtosecond-pulsed phase-shifting digital holography.

    PubMed

    Kakue, Takashi; Itoh, Seiya; Xia, Peng; Tahara, Tatsuki; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2012-08-27

    Parallel phase-shifting digital holography is capable of three-dimensional measurement of a dynamically moving object with a single-shot recording. In this letter, we demonstrated a parallel phase-shifting digital holography using a single femtosecond light pulse whose central wavelength and temporal duration were 800 nm and 96 fs, respectively. As an object, we set spark discharge in atmospheric pressure air induced by applying a high voltage to between two electrodes. The instantaneous change in phase caused by the spark discharge was clearly reconstructed. The reconstructed phase image shows the change of refractive index of air was -3.7 × 10(-4).

  20. Microscopy imaging and quantitative phase contrast mapping in turbid microfluidic channels by digital holography.

    PubMed

    Paturzo, Melania; Finizio, Andrea; Memmolo, Pasquale; Puglisi, Roberto; Balduzzi, Donatella; Galli, Andrea; Ferraro, Pietro

    2012-09-07

    We show that sharp imaging and quantitative phase-contrast microcopy is possible in microfluidics in flowing turbid media by digital holography. In fact, in flowing liquids with suspended colloidal particles, clear vision is hindered and cannot be recovered by any other microscopic imaging technique. On the contrary, using digital holography, clear imaging is possible thanks to the Doppler frequency shift experienced by the photons scattered by the flowing colloidal particles, which do not contribute to the interference process, i.e. the recorded hologram. The method is illustrated and imaging results are demonstrated for pure phase objects, i.e. biological cells in microfluidic channels.

  1. A method for detecting the best reconstructing distance in phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Cao, Wen-Bo; Su, Ping; Ma, Jian-She; Liang, Xian-Ting

    2014-09-01

    In this paper, we propose a novel method to detect the best reconstructing distance in phase-shifting digital holography, which can help one to reconstruct high-quality images even though the recording distance is unkonwn. This scheme is based on an algorithm, two-dimensional discrete cosine transform (DCT). Numerical experiments for this method are shown in this paper. It is shown that this method is not only effective but also fast compared to previous schemes for detecting the focal distance in digital holography. Meanwhile, the algorithm can be effective against different types of noise.

  2. Single-shot two-step Gouy phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Lu, Xiaowei; Li, Jingzhen; Xu, Shixiang; Chen, Hongyi; Wu, Qingyang

    2013-12-01

    We demonstrated a single-shot quasi-on-axis digital holography which is capable of simultaneously capturing two-step phase-shifting interferences. A dual-channel interferometer was employed to monitor the Gouy phase-shifting between two orthogonal polarized references which was introduced by two confocal lenses. A new algorithm was derived for reconstruction the complex field of the object's wavefront according to the feature of Gouy phase-shifting. Simulation was carried out and recover software was also made. The proposed approach can also be applied to single-shot quasi-onaxis digital holography for real time measurement.

  3. Evaluation of parallel phase-shifting digital holography by photon-counting method

    NASA Astrophysics Data System (ADS)

    Miao, Lin; Nitta, Kouichi; Matoba, Osamu; Awatsuji, Yasuhiro

    2012-11-01

    Minimum optical energy required for parallel four-step phase-shifting digital holography is evaluated numerically by using photon-counting method. One of the attractive features of parallel phase-shifting digital holography is the instantaneous recording of fast 3D events where only the complex amplitude distribution of an object wave is obtained. The reconstruction is executed by numerical wave propagation such as angular spectrum propagation or Fresnel propagation. Numerical results indicate that required optical energy of an input image with 512 × 512 pixels is about 11 pJ. Under the criteria used in the evaluation, the required optical energy is independent of the image size.

  4. A phase-shifting in-line digital holography of pre-magnification on imaging research

    NASA Astrophysics Data System (ADS)

    Lin, Qiaowen; Wang, Dayong; Rong, Lu; Wang, Yunxin; Zhao, Jie; Panezai, Spozmai

    2013-12-01

    A phase shifting digital holography with pre-magnification is designed. In order to fully utilize the bandwidth of the camera, a four-step phase-shifting digital holography is adopted to retrieve the complex distribution of the object. To further enhance the resolution of the reconstructed image without phase aberration, two microscope objectives (MOs) are placed in front of the object and the reference mirror. The MO in the reference arm provides parallel beam at the PZT plane thus improve the precision of the phase shifting. A 1951 USAF negative resolution target is used as the sample. Experiment result demonstrates the feasibility of the proposed method.

  5. High-speed phase imaging by parallel phase-shifting digital holography.

    PubMed

    Kakue, Takashi; Yonesaka, Ryosuke; Tahara, Tatsuki; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2011-11-01

    Parallel phase-shifting digital holography can obtain three-dimensional information of a dynamically moving object with high accuracy by using space-division multiplexing of multiple holograms required for phase-shifting interferometry. We demonstrated high-speed parallel phase-shifting digital holography and obtained images of the phase variation of air caused by a compressed gas flow sprayed from a nozzle. In particular, we found the interesting phenomenon of periodic phase distributions. Reconstructed images were obtained at frame rates of 20,000 and 180,000 frames per second.

  6. Suppression of image autocorrelation artefacts in spectral domain optical coherence tomography and multiwave digital holography

    SciTech Connect

    Gelikonov, V M; Gelikonov, G V; Terpelov, D A; Shabanov, D V; Shilyagin, P A

    2012-05-31

    An improved method for suppressing image artefacts in spectral domain optical coherence tomography (SD OCT) and multiwave digital holography, caused by the influence of coherent noise in the course of successive registration of an autocorrelation component and informative signal is reported. The method allows complete suppression of all types of coherent noises, provided that the sample of values used to record the autocorrelation component satisfies the conditions of Kotelnikov's theorem: in SD OCT - for the transverse structure of the studied medium, in multiwave digital holography - for the envelop function of the radiation source frequency tuning spectrum.

  7. Fingerprint biometry applications of digital holography and low-coherence interferography.

    PubMed

    Potcoava, Mariana C; Kim, Myung K

    2009-12-01

    We use several holographic and interferographic methods for two- and three-dimensional imaging of fingerprints. Holographic phase microscopy is used to produce images of thin-film patterns left by latent fingerprints. Two or more holographic phase images with different wavelengths are combined for optical phase unwrapping of images of thicker patent prints or a plastic print. Digital interference holography uses scanned wavelengths to synthesize short-coherence interference tomographic images of a plastic print. We also demonstrate light-emitting-diode-based low-coherence interferography for imaging plastic as well as latent prints. These demonstrations point to significant contributions to biometry by the emerging technology of digital holography and interferography.

  8. Optimization of a lensless digital holographic otoscope system for transient measurements of the human tympanic membrane

    PubMed Central

    Dobrev, I.; Furlong, C.; Cheng, J. T.; Rosowski, J. J.

    2014-01-01

    In this paper, we propose a multi-pulsed double exposure (MPDE) acquisition method to quantify in full-field-of-view the transient (i.e., >10 kHz) acoustically induced nanometer scale displacements of the human tympanic membrane (TM or eardrum). The method takes advantage of the geometrical linearity and repeatability of the TM displacements to enable high-speed measurements with a conventional camera (i.e., <20 fps). The MPDE is implemented on a previously developed digital holographic system (DHS) to enhance its measurement capabilities, at a minimum cost, while avoiding constraints imposed by the spatial resolutions and dimensions of high-speed (i.e., >50 kfps) cameras. To our knowledge, there is currently no existing system to provide such capabilities for the study of the human TM. The combination of high temporal (i.e., >50 kHz) and spatial (i.e., >500k data points) resolutions enables measurements of the temporal and frequency response of all points across the surface of the TM simultaneously. The repeatability and accuracy of the MPDE method are verified against a Laser Doppler Vibrometer (LDV) on both artificial membranes and ex-vivo human TMs that are acoustically excited with a sharp (i.e., <100 μs duration) click. The measuring capabilities of the DHS, enhanced by the MPDE acquisition method, allow for quantification of spatially dependent motion parameters of the TM, such as modal frequencies, time constants, as well as inferring local material properties. PMID:25780271

  9. Optimization of a lensless digital holographic otoscope system for transient measurements of the human tympanic membrane.

    PubMed

    Dobrev, I; Furlong, C; Cheng, J T; Rosowski, J J

    2015-02-01

    In this paper, we propose a multi-pulsed double exposure (MPDE) acquisition method to quantify in full-field-of-view the transient (i.e., >10 kHz) acoustically induced nanometer scale displacements of the human tympanic membrane (TM or eardrum). The method takes advantage of the geometrical linearity and repeatability of the TM displacements to enable high-speed measurements with a conventional camera (i.e., <20 fps). The MPDE is implemented on a previously developed digital holographic system (DHS) to enhance its measurement capabilities, at a minimum cost, while avoiding constraints imposed by the spatial resolutions and dimensions of high-speed (i.e., >50 kfps) cameras. To our knowledge, there is currently no existing system to provide such capabilities for the study of the human TM. The combination of high temporal (i.e., >50 kHz) and spatial (i.e., >500k data points) resolutions enables measurements of the temporal and frequency response of all points across the surface of the TM simultaneously. The repeatability and accuracy of the MPDE method are verified against a Laser Doppler Vibrometer (LDV) on both artificial membranes and ex-vivo human TMs that are acoustically excited with a sharp (i.e., <100 μs duration) click. The measuring capabilities of the DHS, enhanced by the MPDE acquisition method, allow for quantification of spatially dependent motion parameters of the TM, such as modal frequencies, time constants, as well as inferring local material properties.

  10. Acquiring multi-viewpoint image of 3D object for integral imaging using synthetic aperture phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Jeong, Min-Ok; Kim, Nam; Park, Jae-Hyeung; Jeon, Seok-Hee; Gil, Sang-Keun

    2009-02-01

    We propose a method generating elemental images for the auto-stereoscopic three-dimensional display technique, integral imaging, using phase-shifting digital holography. Phase shifting digital holography is a way recording the digital hologram by changing phase of the reference beam and extracting the complex field of the object beam. Since all 3D information is captured by the phase-shifting digital holography, the elemental images for any specifications of the lens array can be generated from single phase-shifting digital holography. We expanded the viewing angle of the generated elemental image by using the synthetic aperture phase-shifting digital hologram. The principle of the proposed method is verified experimentally.

  11. Digital holography with electron wave: measuring into the nanoworld

    NASA Astrophysics Data System (ADS)

    Mendoza Santoyo, Fernando; Voelkl, Edgar

    2016-04-01

    Dennis Gabor invented Holography in 1949. His main concern at the time was centered on the spherical aberration correction in the recently created electron microscopes, especially after O. Scherzer had shown mathematically that round electron optical lenses always have a positive spherical aberration coefficient and the mechanical requirements for minimizing the spherical aberration were too high to allow for atomic resolution. At the time the lack of coherent electron sources meant that in-line holography was developed using quasi-coherent light sources. As such Holography did not produce scientific good enough results to be considered a must use tool. In 1956, G. Moellenstedt invented a device called a wire-biprism that allowed the object and reference beams to be combined in an off-axis configuration. The invention of the laser at the end of the 1950s gave a great leap to Holography since this light source was highly coherent and hence led to the invention of Holographic Interferometry during the first lustrum of the 1960s. This new discipline in the Optics field has successfully evolved to become a trusted tool in a wide variety of areas. Coherent electron sources were made available only by the late 1970s, a fact that gave an outstanding impulse to electron holography so that today nanomaterials and structures belonging to a wide variety of subjects can be characterized in regards to their physical and mechanical parameters. This invited paper will present and discuss electron holography's state of the art applications to study the shape of nanoparticles and bacteria, and the qualitative and quantitative study of magnetic and electric fields produced by novel nano-structures.

  12. Two-step-only quadrature phase-shifting digital holography.

    PubMed

    Liu, Jung-Ping; Poon, Ting-Chung

    2009-02-01

    Conventional methods of quadrature phase-shifting holography require two holograms and either intensity distribution of the reference wave or that of the object wave to reconstruct an original object without the zero order and the twin-image noise in an on-axis holographic recording setup. We present a technique called two-step-only quadrature phase-shifting holography in which solely two quadrature-phase holograms are required. Neither reference-wave intensity nor an object-wave intensity measurement is needed in the technique.

  13. Doppler phase-shifting digital holography and its application to surface shape measurement.

    PubMed

    Kikuchi, Yuichi; Barada, Daisuke; Kiire, Tomohiro; Yatagai, Toyohiko

    2010-05-15

    Digital holography utilizing the optical Doppler effect is proposed in which the time variation of interference fringes is recorded using a high-speed CMOS camera. The complex amplitude diffracted from the object wave is extracted by time-domain Fourier transforming the recorded interference fringes. The method was used to measure the surface shape of a concave mirror under a disturbed environment.

  14. Twin-image reduction method for in-line digital holography using periphery and random reference phase-shifting techniques

    NASA Astrophysics Data System (ADS)

    Oshima, Teppei; Matsudo, Yusuke; Kakue, Takashi; Arai, Daisuke; Shimobaba, Tomoyoshi; Ito, Tomoyoshi

    2015-09-01

    Digital holography has the twin image problem that unwanted lights (conjugate and direct lights) overlap in the object light in the reconstruction process. As a method for extracting only the object light, phase-shifting digital holography is widely used; however, this method is not applicable for the observation of moving objects, because this method requires the recording of plural holograms. In this study, we propose a twin-image reduction method by combining the "periphery" method with the "random phase-shifting" method. The proposed method succeeded in improving the reconstruction quality, compared to other one-shot recording methods ("parallel phase-shifting digital holography" and "random phase-shifting").

  15. Investigations of cloud microphysical response to mixing using digital holography

    NASA Astrophysics Data System (ADS)

    Beals, Matthew Jacob

    Cloud edge mixing plays an important role in the life cycle and development of clouds. Entrainment of subsaturated air affects the cloud at the microscale, altering the number density and size distribution of its droplets. The resulting effect is determined by two timescales: the time required for the mixing event to complete, and the time required for the droplets to adjust to their new environment. If mixing is rapid, evaporation of droplets is uniform and said to be homogeneous in nature. In contrast, slow mixing (compared to the adjustment timescale) results in the droplets adjusting to the transient state of the mixture, producing an inhomogeneous result. Studying this process in real clouds involves the use of airborne optical instruments capable of measuring clouds at the 'single particle' level. Single particle resolution allows for direct measurement of the droplet size distribution. This is in contrast to other 'bulk' methods (i.e. hot-wire probes, lidar, radar) which measure a higher order moment of the distribution and require assumptions about the distribution shape to compute a size distribution. The sampling strategy of current optical instruments requires them to integrate over a path tens to hundreds of meters to form a single size distribution. This is much larger than typical mixing scales (which can extend down to the order of centimeters), resulting in difficulties resolving mixing signatures. The Holodec is an optical particle instrument that uses digital holography to record discrete, local volumes of droplets. This method allows for statistically significant size distributions to be calculated for centimeter scale volumes, allowing for full resolution at the scales important to the mixing process. The hologram also records the three dimensional position of all particles within the volume, allowing for the spatial structure of the cloud volume to be studied. Both of these features represent a new and unique view into the mixing problem. In

  16. A method of hard X-ray phase-shifting digital holography.

    PubMed

    Park, So Yeong; Hong, Chung Ki; Lim, Jun

    2016-07-01

    A new method of phase-shifting digital holography is demonstrated in the hard X-ray region. An in-line-type phase-shifting holography setup was installed in a 6.80 keV hard X-ray synchrotron beamline. By placing a phase plate consisting of a hole and a band at the focusing point of a Fresnel lens, the relative phase of the reference and objective beams could be successfully shifted for use with a three-step phase-shift algorithm. The system was verified by measuring the shape of a gold test pattern and a silica sphere.

  17. Real-time visualization of Karman vortex street in water flow field by using digital holography.

    PubMed

    Sun, Weiwei; Zhao, Jianlin; Di, Jianglei; Wang, Qian; Wang, Le

    2009-10-26

    The Karman vortex street generated behind a circular cylinder in water flow field is displayed and analyzed in real time by means of digital holography. Using a modified Mach-Zehnder interferometer, a digital hologram of the flow field in still state and then a video of continuous digital holograms in flowing state are recorded at 14.6 frames per second by a CCD camera, respectively. A series of sequential phase maps of the flow field are numerically reconstructed from the holograms in different states above based on double-exposure holographic interferometry. By seriating these phase maps, the shape and evolution of Karman vortex street can be displayed in real time in the form of a movie. For comparison, numerical simulation of the Karman vortex street under the boundary conditions adopted in the experiment is also presented, and the consistent results indicate that the experimental observation of Karman vortex street by using digital holography is successful and feasible.

  18. Image multiplexing and encryption using the nonnegative matrix factorization method adopting digital holography.

    PubMed

    Chang, Hsuan T; Shui, J-W; Lin, K-P

    2017-02-01

    In this paper, a joint multiple-image encryption and multiplexing system, which utilizes both the nonnegative matrix factorization (NMF) scheme and digital holography, is proposed. A number of images are transformed into noise-like digital holograms, which are then decomposed into a defined number of basis images and a corresponding weighting matrix using the NMF scheme. The determined basis images are similar to the digital holograms and appear as noise-like patterns, which are then stored as encrypted data and serve as the lock in an encryption system. On the other hand, the column vectors in the weighting matrix serve as the keys for the corresponding plain images or the addresses of the multiplexed images. Both the increased uniformity of the column weighting factors and the parameters used in the digital holography enhance the security of the distributed keys. The experimental results show that the proposed method can successfully perform multiple-image encryption with high-level security.

  19. A comparison of filtering techniques on denoising terahertz coaxial digital holography image

    NASA Astrophysics Data System (ADS)

    Cui, Shan-shan; Li, Qi

    2016-10-01

    In the process of recording terahertz digital hologram, the hologram is easy to be contaminated by speckle noise, which leads to lower resolution in imaging system and affects the reconstruction results seriously. Thus, the study of filtering algorithms applicable for de-speckling terahertz digital holography image has important practical values. In this paper, non-local means filtering and guided bilateral filtering were brought to process the real image reconstructed from continuous-wave terahertz coaxial digital hologram. For comparison, median filtering, bilateral filtering, and robust bilateral filtering, were introduced as conventional methods to denoise the real image. Then, all the denoising results were evaluated. The comparison indicates that the guided bilateral filter manifests the optimal denoising effect for the terahertz digital holography image, both significantly suppressing speckle noise, and effectively preserving the useful information on the reconstructed image.

  20. Measuring a thermal expansion of thermoelectric materials by using in-line digital holography

    NASA Astrophysics Data System (ADS)

    Thong-on, Thanyarat; Buranasiri, Prathan

    2016-10-01

    In this paper, thermal expansion measurement of thermoelectric materials has been done using digital holography technique. In the experimental setup, a diode laser, a digital camera and a sample on a hot plate were put in the same alignment, so it is call Digital in-line Holography (DIH). A laser beam was expanded parallel and then propagated through a thermoelectric sample which would be heated by a hot plate from a room temperature to 224 °C. The images of a TE sample were recorded by a digital camera and analyzed data by numerical image reconstruction. From our experimental measurement result, thermoelectric material was expanded with temperature slightly, and its thermal expansion coefficient (COE) was found equal to αTE = 2.25 × 10-6 °C-1.

  1. Comparative analysis of mathematical models of the matrix photodetector used in digital holography

    NASA Astrophysics Data System (ADS)

    Grebenyuk, K. A.

    2017-08-01

    It is established, that in modern works on digital holography, three fundamentally different mathematical models of a matrix photodetector are used. Comparative analysis of these models, including analysis of the formula of each model and test calculations, has been conducted. The possibility of using these models to account for the influence of geometrical parameters of a matrix photodetector on the properties of recorded digital holograms is considered.

  2. Numerical twin image suppression by nonlinear segmentation mask in digital holography.

    PubMed

    Cho, ChoongSang; Choi, ByeongHo; Kang, HoonJong; Lee, SangKeun

    2012-09-24

    The in-line holography has obvious advantages especially in wider spatial bandwidth over the off-axis holography. However, a direct current(DC)-noise and an unwanted twin image should be separated or eliminated in the in-line holography for a high quality reconstruction. An approach for suppressing the twin image is proposed by separating the real and twin image regions in the digital holography. Specifically, the initial region of real and twin images is obtained by a blind separation matrix, and the segmentation mask to suppress the twin image is calculated by nonlinear quantization from the segmented image. For the performance evaluation, the proposed method is compared with the existing approaches including the overlapping block variance and manual-based schemes. Experimental results showed that the proposed method has a better performance at the overlapped region of the real and twin images. Additionally, the proposed method causes less loss of real image than the overlapping block variance-based scheme. Therefore, we believe that the proposed scheme can be a useful tool for high quality reconstruction in the in-line holography.

  3. Expansion of field of view in digital in-line holography with a programmable point source.

    PubMed

    Adeyemi, Adekunle A; Darcie, Thomas E

    2009-06-20

    We present a technique for programming the source of the spherical reference illumination in digital in-line holography using digital micromirror devices. The programmable point source is achieved by individually addressing the elements of a digital micromirror device to spatially control the illumination of the object located at some distance from the source of the spherical reference field. By moving the location of the "ON" element on the digital micromirror device, translation of both the source of the spherical reference beam and the captured holograms is achieved. Results obtained through numerical reconstruction of these translated holograms shows the possibility of expanding the field of view by about 263%.

  4. Digital holography for coherent fiber beam combining with a co-propagative scheme.

    PubMed

    Antier, Marie; Larat, Christian; Lallier, Eric; Bourderionnet, Jérôme; Primot, Jérôme; Brignon, Arnaud

    2014-09-22

    We present a technique for passive coherent fiber beam combining based on digital holography. In this method, the phase errors between the fibers are compensated by the diffracted phase-conjugated -1 order of a digital hologram. Unlike previous digital holography technique, the probe beams measuring the phase errors between the fibers are co-propagating with the phase-locked signal beams. This architecture is compatible with the use of multi-stage isolated amplifying fibers. It does not require any phase calculation algorithm and its correction is collective. This concept is experimentally demonstrated with three fibers at 1.55 μm. A residual phase error of λ/20 is measured.

  5. Statistical generalized phase-shifting digital holography with a continuous fringe-scanning scheme.

    PubMed

    Yoshikawa, Nobukazu; Kajihara, Kazuki

    2015-07-01

    We propose a novel statistical generalized phase-shifting digital holography using a continuous fringe-scanning scheme. In this method, the continuous fringe-scanning scheme is implemented using a PC-based measurement system without any synchronous circuit between the digital camera and the phase shifter. Thus, nonuniformly phase-shifted interference fringes are captured sequentially because of the fluctuation of the image-capturing interval. To cope with the nonuniform phase shifts, we employ a statistical generalized phase-shifting approach. Since the algorithm is designed to use an arbitrary phase shift, the nonuniform phase shifts do not obstruct object wave retrieval. Simulations and experiments demonstrate that the proposed method can be used to implement a practical and accurate digital holography system.

  6. A novel method for identifying the order of interference using phase-shifting digital holography.

    PubMed

    Sokkar, T Z N; El-Farahaty, K A; Ramadan, W A; Wahba, H H; Raslan, M I; Hamza, A A

    2016-04-01

    In this paper, we introduced a mathematical method for measuring the optical path length differences (OPDs), which is suitable for large OPD values where the fringes connections are difficult to detect. The proposed method is based on varying the width of the fringes, without changing the wavelength of the used coherent source. Also, in this work, we discussed the need for such method in off-axis phase-shifting digital holography. Low-resolution off-axis holograms failed to detect the correct interference order. In general, off-axis phase-shifting digital holography is limited by the resolution of the captured holograms. The results obtained using our proposed technique were compared to the results obtained using off-axis phase-shifting digital holograms and conventional two-beam interferometry. Holograms were given for illustration. © 2015 The Authors Journal of Microscopy © 2015 Royal Microscopical Society.

  7. Inverse-problem approach for particle digital holography: accurate location based on local optimization.

    PubMed

    Soulez, Ferréol; Denis, Loïc; Fournier, Corinne; Thiébaut, Eric; Goepfert, Charles

    2007-04-01

    We propose a microparticle localization scheme in digital holography. Most conventional digital holography methods are based on Fresnel transform and present several problems such as twin-image noise, border effects, and other effects. To avoid these difficulties, we propose an inverse-problem approach, which yields the optimal particle set that best models the observed hologram image. We resolve this global optimization problem by conventional particle detection followed by a local refinement for each particle. Results for both simulated and real digital holograms show strong improvement in the localization of the particles, particularly along the depth dimension. In our simulations, the position precision is > or =1 microm rms. Our results also show that the localization precision does not deteriorate for particles near the edge of the field of view.

  8. Color digital holography using speckle illumination by means of a multi-mode fiber

    NASA Astrophysics Data System (ADS)

    Funamizu, Hideki; Shimoma, Shohei; Aizu, Yoshihisa

    2014-02-01

    We present color digital holography using speckle illumination by means of a multi-mode fiber. In this technique, speckle fields emitted from the fiber are used as both a reference wave and a wavefront illuminating an object. For three wavelengths, the interference patterns of two coherent waves are recorded as digital holograms on a CCD camera. A speckle method is used for suppressing DC terms and reducing a twin image in an in-line color digital holography. The speckle fields are changed by vibrating the multi-mode fiber using a vibrator, and a number of holograms are acquired to average reconstructed images. The dependence of the averaged number of holograms on color quality of reconstructed images is evaluated by chromaticity coordinates and color differences in colorimetry.

  9. Security enhanced optical one-time password authentication method by using digital holography

    NASA Astrophysics Data System (ADS)

    Gil, Sang Keun; Jeon, Seok Hee; Jeong, Jong Rae

    2015-03-01

    We propose a new optical one-time password(OTP) authentication method by using digital holography, which enhances security strength in the cryptosystem compared to the conventional electronic OTP method. In this paper, a challenge-response optical OTP authentication based on two-factor authentication is presented by 2-step quadrature phase-shifting digital holography using orthogonal polarization, and two-way authentication is also performed using the challenge-response handshake in both directions. The ID (identification), PW (password) and OTP information are encrypted with a shared key by applying phase-shifting digital holography, and these encrypted information are verified each other by the shared key. Because the encrypted digital holograms which are transmitted to the other party are expressed as random distribution, it guards against a replay attack and results in higher security level. Optically, encrypted digital hologram in our method is Fourier transform hologram and is recorded on CCD with 256 gray-level quantized intensities. The proposed method has an advantage that it does not need a time-synchronized OTP and can be applied to various security services. Computer experiments show that the proposed method is suitable for high secure OTP authentication.

  10. Experimental imaging research on continuous-wave terahertz in-line digital holography

    NASA Astrophysics Data System (ADS)

    Huang, Haochong; Wang, Dayong; Rong, Lu; Wang, Yunxin

    2014-09-01

    The terahertz (THz) imaging is an advanced technique on the basis of the unique characteristics of terahertz radiation. Due to its noncontact, non-invasive and high-resolution capabilities, it has already shown great application prospects in biomedical observation, sample measurement, and quality control. The continuous-wave terahertz in-line digital holography is a combination of terahertz technology and in-line digital holography of which the source is a continuous-wave terahertz laser. Over the past decade, many researchers used different terahertz sources and detectors to undertake experiments. In this paper, the pre-process of the hologram is accomplished after the holograms' recording process because of the negative pixels in the pyroelectric detector and the air vibration caused by the chopper inside the camera. To improve the quality of images, the phase retrieval algorithm is applied to eliminate the twin images. In the experiment, the pin which terahertz wave can't penetrate and the TPX slice carved letters "THz" are chosen for the samples. The amplitude and phase images of samples are obtained and the twin image and noise in the reconstructed images are suppressed. The results validate the feasibility of the terahertz in-line digital holographic imaging technique. This work also shows the terahertz in-line digital holography technique's prospects in materials science and biological samples' detection.

  11. Real-time visualization and analysis of airflow field by use of digital holography

    NASA Astrophysics Data System (ADS)

    Di, Jianglei; Wu, Bingjing; Chen, Xin; Liu, Junjiang; Wang, Jun; Zhao, Jianlin

    2013-04-01

    The measurement and analysis of airflow field is very important in fluid dynamics. For airflow, smoke particles can be added to visually observe the turbulence phenomena by particle tracking technology, but the effect of smoke particles to follow the high speed airflow will reduce the measurement accuracy. In recent years, with the advantage of non-contact, nondestructive, fast and full-field measurement, digital holography has been widely applied in many fields, such as deformation and vibration analysis, particle characterization, refractive index measurement, and so on. In this paper, we present a method to measure the airflow field by use of digital holography. A small wind tunnel model made of acrylic glass is built to control the velocity and direction of airflow. Different shapes of samples such as aircraft wing and cylinder are placed in the wind tunnel model to produce different forms of flow field. With a Mach-Zehnder interferometer setup, a series of digital holograms carrying the information of airflow filed distributions in different states are recorded by CCD camera and corresponding holographic images are numerically reconstructed from the holograms by computer. Then we can conveniently obtain the velocity or pressure information of the airflow deduced from the quantitative phase information of holographic images and visually display the airflow filed and its evolution in the form of a movie. The theory and experiment results show that digital holography is a robust and feasible approach for real-time visualization and analysis of airflow field.

  12. Lensless x-ray imaging in reflection geometry

    SciTech Connect

    Roy, S.; Parks, D.H.; Seu, K.A.; Turner, J.J.; Chao, W.; Anderson, E.H.; Cabrini, S.; Kevan, S.D.; Su, R.

    2011-02-03

    Lensless X-ray imaging techniques such as coherent diffraction imaging and ptychography, and Fourier transform holography can provide time-resolved, diffraction-limited images. Nearly all examples of these techniques have focused on transmission geometry, restricting the samples and reciprocal spaces that can be investigated. We report a lensless X-ray technique developed for imaging in Bragg and small-angle scattering geometries, which may also find application in transmission geometries. We demonstrate this by imaging a nanofabricated pseudorandom binary structure in small-angle reflection geometry. The technique can be used with extended objects, places no restriction on sample size, and requires no additional sample masking. The realization of X-ray lensless imaging in reflection geometry opens up the possibility of single-shot imaging of surfaces in thin films, buried interfaces in magnetic multilayers, organic photovoltaic and field-effect transistor devices, or Bragg planes in a single crystal.

  13. Direct inversion of digital 3D Fraunhofer holography maps.

    PubMed

    Podorov, Sergei G; Förster, Eckhart

    2016-01-20

    Differential Fourier holography (DFH) gives an exact mathematical solution of the inverse problem of diffraction in the Fraunhofer regime. After the first publication [Opt. Express15, 9954 (2007)], DFH was successfully applied in many experiments to obtain amplitude and phase information about two-dimensional images. In this paper, we demonstrate numerically the possibility to apply DFH also for investigation of unknown three-dimensional objects. The first simulation is made for a double-spiral structure plus a line as a reference object.

  14. Fourier transform light scattering angular spectroscopy using digital inline holography.

    PubMed

    Kim, Kyoohyun; Park, YongKeun

    2012-10-01

    A simple and practical method for measuring the angle-resolved light scattering (ARLS) from individual objects is reported. Employing the principle of inline holography and a Fourier transform light scattering technique, both the static and dynamic scattering patterns from individual micrometer-sized objects can be effectively and quantitatively obtained. First, the light scattering measurements were performed on individual polystyrene beads, from which the refractive index and diameter of each bead were retrieved. Also, the measurements of the static and dynamic light scattering from intact human red blood cells are demonstrated. Using the present method, an existing microscope can be directly transformed into a precise instrument for ARLS measurements.

  15. Removal of residual images in parallel phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Tahara, Tatsuki; Shimozato, Yuki; Xia, Peng; Ito, Yasunori; Kakue, Takashi; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2013-01-01

    Parallel phase-shifting digital holography (PPSDH) is a technique of single-shot phase-shifting digital holography. We found that there are two problems with this technique. (1) Some extraneous noises caused by the intensity unevenness of the reference wave become slightly superimposed on the object image. (2) The conjugate image cannot be completely removed. This is because the object wave causes the phase-shift error by illuminating an image sensor with a large incident angle. To solve these problems, we propose an algorithm for removing residual 0th-order diffraction and conjugate images in PPSDH. In the proposed algorithm, we modified phase-shifting interferometry in order to work through the unevenness of the intensity distribution and applied the Fourier transform technique to PPSDH to remove the residual conjugate image. The effectiveness of the proposed algorithm was experimentally verified.

  16. Experimental demonstration of parallel phase-shifting digital holography under weak light condition

    NASA Astrophysics Data System (ADS)

    Miao, Lin; Tahara, Tatsuki; Xia, Peng; Ito, Yasunori; Nitta, Kouichi; Awatsuji, Yasuhiro; Matoba, Osamu

    2014-03-01

    One of advantages of parallel phase-shifting digital holography (PPSDH) compared with other digital holography techniques is the fast recording of three-dimensional (3D) objects. During the fast recording of a multiplexed hologram that contains at least three amounts of phase retardation, the optical energy of the hologram becomes smaller. Therefore, it is important to assess the minimum optical energy that can reconstruct the object with moderate reconstruction error. In this paper, we investigate experimentally the optical energy to reconstruct the object under weak light condition in PPSDH. We compare the numerical and experimental results. The experiment is in good agreement with numerical results when the sensitivity of the image sensor is taken into account.

  17. Parallel phase-shifting color digital holography using two phase shifts.

    PubMed

    Kakue, Takashi; Tahara, Tatsuki; Ito, Kenichi; Shimozato, Yuki; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2009-12-01

    We propose parallel phase-shifting color digital holography using two phase shifts. This technique enables the instantaneous acquisition of three-dimensional information of a moving color object. The interference fringe image that contains six holograms with two phase shifts for three wavelengths is recorded by a single shot exposure. Decreasing the degree of space-division multiplexing of these holograms makes it possible to suppress the degradation of the image quality owing to the aliasing caused by the multiplexing. Numerical simulation and preliminary experiments demonstrate the validity of the proposed technique; the reconstructed images of the proposed technique are clearer than those of the previously reported single-shot phase-shifting color digital holography that uses four phase steps.

  18. Experimental demonstration of parallel two-step phase-shifting digital holography.

    PubMed

    Tahara, Tatsuki; Ito, Kenichi; Fujii, Motofumi; Kakue, Takashi; Shimozato, Yuki; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2010-08-30

    Parallel two-step phase-shifting digital holography is a technique for single-shot implementation of phase-shifting interferometry and requires only the intensity distribution of the reference wave and spatial two phase-shifted holograms. We constructed a system of parallel two-step phase-shifting digital holography and experimentally demonstrated the technique, for the first time. The system uses an originally fabricated image sensor having an array of 2 × 1 micro polarizers. Each micro polarizer was attached on pixel by pixel. In the experiment, the unwanted images, the zero-order diffraction wave and the conjugate image, are removed from the reconstructed image of objects by the system, while the images superimpose on the image of objects reconstructed by Fresnel transform alone. Also the capability of single-shot and three-dimensional imaging is demonstrated by the system.

  19. Digital holography-secured scheme using only binary phase or amplitude as ciphertext.

    PubMed

    Chen, Wen; Chen, Xudong

    2016-08-20

    A digital holography-secured scheme is presented by using binary phase or amplitude. The input image is encrypted based on double random phase encoding, and a complex-valued wavefront in the charge-coupled device plane is extracted by using digital holography. Subsequently, only the phase component of the extracted complex-valued wavefront is maintained, and is further binarized. Different from conventional methods, an interesting finding in this paper is that in addition to binary phase, binary amplitude originating from the binarized phase pattern can also be applied as ciphertext. During optical decoding, the decrypted image cannot visually render clear information about the input, and the authentication is further conducted. The binary phase or amplitude pattern can be flexibly applied as ciphertext, and the fully optical approach can be implemented for the decoding. The ciphertext is effectively compressed, which can facilitate the storage and transmission in practical applications.

  20. Detection of inhomogeneities in a metal cylinder using ESPI and 3D pulsed digital holography

    NASA Astrophysics Data System (ADS)

    Saucedo-Anaya, Tonatiuh; Mendoza Santoyo, Fernando; Perez-Lopez, Carlos; de la Torre Ibarra, Manuel

    2004-06-01

    ESPI and 3D pulsed Digital Holography have been applied to detect inhomogeneities inside a metal cylinder. A shaker was employed to produce a mechanical wave that propagates through the inner structure of the cylinder in such a way that it generates vibrational resonant modes on the cylinder surface. An out of plane ESPI optical sensitive configuration was used to detect vibrational resonant modes. A 3D multi-pulse digital holography system was used to obtain quantitative deformation data of the dynamically moving cylinder. The local decrease in structural stiffness inside the cylinder due to an inhomogeneity produces an asymmetry in the resonant mode shape. Results show that the inhomogeneity produces an asymmetry in its vibrational resonant modes. The method may be reliably used to study and compare data from inside homogeneous and inhomogeneous solid materials.

  1. Single-shot incoherent digital holography using a dual-focusing lens with diffraction gratings.

    PubMed

    Quan, Xiangyu; Matoba, Osamu; Awatsuji, Yasuhiro

    2017-02-01

    A new optical configuration of incoherent digital holography is presented to improve the quality of reconstructed images when the random polarization state of incoherent light is used. The proposed system improves the signal-to-noise ratio of the holograms by suppressing the unmodulated terms of a spatial light modulator. To generate the self-interference of a quasi-incoherent point-like source, we use a dual-focusing lens with diffraction gratings. The preliminary experimental results confirm the validity of the proposed method by reconstructing two point-like sources generated by a LED light source. When the pixel pitch of the phase-mode SLM is small enough, the off-axis hologram can be generated. The single-shot recording of the incoherent digital holography is expected.

  2. Phase reconstruction of digital holography with the peak of the two-dimensional Gabor wavelet transform.

    PubMed

    Weng, Jiawen; Zhong, Jingang; Hu, Cuiying

    2009-06-20

    We describe a numerical reconstruction technique for digital holography by means of the two-dimensional Gabor wavelet transform (2D-GWT). Applying the 2D-GWT to digital holography, the object wave can be reconstructed by calculating the wavelet coefficients of the hologram at the peak of the 2D-GWT automatically. At the same time the effect of the zero-order diffraction image and the twin image are eliminated without spatial filtering. Comparing the numerical reconstruction of a holographic image by the analysis of the one-dimensional Gabor wavelet transform (1D-GWT) with the 2D-GWT, we show that the 2D-GWT method is superior to the 1D-GWT method, especially when the fringes of the hologram are not just along the y direction. The theory and the results of a simulation and experiments are shown.

  3. Investigation of super-resolution processing algorithm by target light-intensity search in digital holography

    NASA Astrophysics Data System (ADS)

    Neo, Atsushi; Kakue, Takashi; Shimobaba, Tomoyoshi; Masuda, Nobuyuki; Ito, Tomoyoshi

    2017-04-01

    Digital holography is expected to be useful in the analysis of moving three-dimensional (3D) image measurement. In this technique, a two-dimensional interference fringe recorded using a 3D image is captured with an image sensor, and the 3D image is reproduced on a computer. To obtain the reproduced 3D images with high spatial resolution, a high-performance image sensor is required, which increases the system cost. We propose an algorithm for super-resolution processing in digital holography that does not require a high-performance image sensor. The proposed algorithm wherein 3D images are considered as the aggregation of object points improves spatial resolution by performing a light-intensity search of the reproduced image and the object points.

  4. Phase shifting digital holography implemented with a twisted-nematic liquid-crystal display.

    PubMed

    Cruz, Maria-Luisa; Castro, Albertina; Arrizón, Victor

    2009-12-20

    We describe and experimentally demonstrate a phase shifting method based on the lateral displacement of a grating implemented with a twisted-nematic liquid-crystal spatial light modulator. This method allows an accurate implementation of the phase shift without requiring moving parts. The technique is implemented in a Mach-Zehnder digital holography setup in which the field transmitted by the sample object freely propagates to the hologram plane.

  5. Phase-shifting error and its elimination in phase-shifting digital holography.

    PubMed

    Guo, Cheng-Shan; Zhang, Li; Wang, Hui-Tian; Liao, Jun; Zhu, Y Y

    2002-10-01

    We investigate the influence of phase-shifting error on the quality of the reconstructed image in digital holography and propose a method of error elimination for a perfect image. In this method the summation of the intensity bit errors of the reconstructed image is taken as an evaluation function for an iterative algorithm to find the exact phase-shifting value. The feasibility of this method is demonstrated by computer simulation.

  6. Phase-shifting digital holography with a phase difference between orthogonal polarizations.

    PubMed

    Nomura, Takanori; Murata, Shinji; Nitanai, Eiji; Numata, Takuhisa

    2006-07-10

    Phase-shifting digital holography with a phase difference between orthogonal polarizations is proposed. The use of orthogonal polarizations can make it possible to record two phase-shifted holograms simultaneously. By combining the holograms with the distributions of a reference wave and an object wave, the complex field of the object's wavefront can be obtained. Preliminary experimental results are shown to confirm the proposed method.

  7. Digital Holography for in Situ Real-Time Measurement of Plasma-Facing-Component Erosion

    SciTech Connect

    ThomasJr., C. E.; Granstedt, E. M.; Biewer, Theodore M; Baylor, Larry R; Combs, Stephen Kirk; Meitner, Steven J; Hillis, Donald Lee; Majeski, R.; Kaita, R.

    2014-01-01

    In situ, real time measurement of net plasma-facing-component (PFC) erosion/deposition in a real plasma device is challenging due to the need for good spatial and temporal resolution, sufficient sensitivity, and immunity to fringe-jump errors. Design of a high-sensitivity, potentially high-speed, dual-wavelength CO2 laser digital holography system (nominally immune to fringe jumps) for PFC erosion measurement is discussed.

  8. Digital holography for in situ real-time measurement of plasma-facing-component erosion.

    PubMed

    Thomas, C E Tommy; Granstedt, E M; Biewer, T M; Baylor, L R; Combs, S K; Meitner, S J; Hillis, D L; Majeski, R; Kaita, R

    2014-11-01

    In situ, real time measurement of net plasma-facing-component (PFC) erosion/deposition in a real plasma device is challenging due to the need for good spatial and temporal resolution, sufficient sensitivity, and immunity to fringe-jump errors. Design of a high-sensitivity, potentially high-speed, dual-wavelength CO2 laser digital holography system (nominally immune to fringe jumps) for PFC erosion measurement is discussed.

  9. Measurement of the traction force of biological cells by digital holography

    PubMed Central

    Yu, Xiao; Cross, Michael; Liu, Changgeng; Clark, David C.; Haynie, Donald T.; Kim, Myung K.

    2011-01-01

    The traction force produced by biological cells has been visualized as distortions in flexible substrata. We have utilized quantitative phase microscopy by digital holography (DH-QPM) to study the wrinkling of a silicone rubber film by motile fibroblasts. Surface deformation and the cellular traction force have been measured from phase profiles in a direct and straightforward manner. DH-QPM is shown to provide highly efficient and versatile means for quantitatively analyzing cellular motility. PMID:22254175

  10. Focus detection criterion for refocusing in multi-wavelength digital holography.

    PubMed

    Xu, Li; Mater, Mike; Ni, Jun

    2011-08-01

    The majority of focus detection criteria reported is based on amplitude contrast. Due to phase wrapping, phase contrast was previously reported unsuitable for focus finding tasks. By taking the advantage of multi-wavelength digital holography, we propose a new focus detection criterion based on phase contrast. Experimental results are presented to prove the feasibility of the developed criterion. Possible applications of the developed technology include inspecting machined surfaces in the auto industry.

  11. Optical design of cipher block chaining (CBC) encryption mode by using digital holography

    NASA Astrophysics Data System (ADS)

    Gil, Sang Keun; Jeon, Seok Hee; Jung, Jong Rae; Kim, Nam

    2016-03-01

    We propose an optical design of cipher block chaining (CBC) encryption by using digital holographic technique, which has higher security than the conventional electronic method because of the analog-type randomized cipher text with 2-D array. In this paper, an optical design of CBC encryption mode is implemented by 2-step quadrature phase-shifting digital holographic encryption technique using orthogonal polarization. A block of plain text is encrypted with the encryption key by applying 2-step phase-shifting digital holography, and it is changed into cipher text blocks which are digital holograms. These ciphered digital holograms with the encrypted information are Fourier transform holograms and are recorded on CCDs with 256 gray levels quantized intensities. The decryption is computed by these encrypted digital holograms of cipher texts, the same encryption key and the previous cipher text. Results of computer simulations are presented to verify that the proposed method shows the feasibility in the high secure CBC encryption system.

  12. Terahertz digital holography image denoising using stationary wavelet transform

    NASA Astrophysics Data System (ADS)

    Cui, Shan-Shan; Li, Qi; Chen, Guanghao

    2015-04-01

    Terahertz (THz) holography is a frontier technology in terahertz imaging field. However, reconstructed images of holograms are inherently affected by speckle noise, on account of the coherent nature of light scattering. Stationary wavelet transform (SWT) is an effective tool in speckle noise removal. In this paper, two algorithms for despeckling SAR images are implemented to THz images based on SWT, which are threshold estimation and smoothing operation respectively. Denoised images are then quantitatively assessed by speckle index. Experimental results show that the stationary wavelet transform has superior denoising performance and image detail preservation to discrete wavelet transform. In terms of the threshold estimation, high levels of decomposing are needed for better denoising result. The smoothing operation combined with stationary wavelet transform manifests the optimal denoising effect at single decomposition level, with 5×5 average filtering.

  13. Surface shape measurement by phase-shifting digital holography with a wavelength shift.

    PubMed

    Yamaguchi, Ichirou; Ida, Takashi; Yokota, Masayuki; Yamashita, Kouji

    2006-10-10

    Surface contouring by phase-shifting digital holography is proposed and verified by experiments and numerical simulations. Digital holograms are recorded before and after mode hopping of a laser diode subject to current tuning, and the difference of the reconstructed phases at each wavelength is computed to deliver surface contours of a diffusely reflecting surface. Since normal incidence on the object is employed, the method does not need the removal of the tilt component and is free from the shadowing effect as advantages over the dual-incidence method proposed before by the first author.

  14. Single-exposure phase-shifting digital holography using a random-phase reference wave.

    PubMed

    Nomura, Takanori; Imbe, Masatoshi

    2010-07-01

    We propose a single-exposure phase-shifting digital holography based on a wave-splitting method using a random-phase reference wave. A random-phase reference wave gives random-phase distribution on the digital hologram. Using the amplitude and the phase distributions of the reference wave, the fully complex amplitude of the object wave is obtained. The proposed method requires not devised optical systems but ordinary imaging devices, such as CCD cameras. A preliminary experimental result is given to confirm the proposed method.

  15. Measurement of curvature and twist of a deformed object using digital holography

    SciTech Connect

    Chen Wen; Quan Chenggen; Cho Jui Tay

    2008-05-20

    Measurement of curvature and twist is an important aspect in the study of object deformation. In recent years, several methods have been proposed to determine curvature and twist of a deformed object using digital shearography. Here we propose a novel method to determine the curvature and twist of a deformed object using digital holography and a complex phasor. A sine/cosine transformation method and two-dimensional short time Fourier transform are proposed subsequently to process the wrapped phase maps. It is shown that high-quality phase maps corresponding to curvature and twist can be obtained. An experiment is conducted to demonstrate the validity of the proposed method.

  16. Multilevel recording of complex amplitude data pages in a holographic data storage system using digital holography.

    PubMed

    Nobukawa, Teruyoshi; Nomura, Takanori

    2016-09-05

    A holographic data storage system using digital holography is proposed to record and retrieve multilevel complex amplitude data pages. Digital holographic techniques are capable of modulating and detecting complex amplitude distribution using current electronic devices. These techniques allow the development of a simple, compact, and stable holographic storage system that mainly consists of a single phase-only spatial light modulator and an image sensor. As a proof-of-principle experiment, complex amplitude data pages with binary amplitude and four-level phase are recorded and retrieved. Experimental results show the feasibility of the proposed holographic data storage system.

  17. Terahertz digital holography using angular spectrum and dual wavelength reconstruction methods.

    PubMed

    Heimbeck, Martin S; Kim, Myung K; Gregory, Don A; Everitt, Henry O

    2011-05-09

    Terahertz digital off-axis holography is demonstrated using a Mach-Zehnder interferometer with a highly coherent, frequency tunable, continuous wave terahertz source emitting around 0.7 THz and a single, spatially-scanned Schottky diode detector. The reconstruction of amplitude and phase objects is performed digitally using the angular spectrum method in conjunction with Fourier space filtering to reduce noise from the twin image and DC term. Phase unwrapping is achieved using the dual wavelength method, which offers an automated approach to overcome the 2π phase ambiguity. Potential applications for nondestructive test and evaluation of visually opaque dielectric and composite objects are discussed. © 2011 Optical Society of America

  18. Parallel phase-shifting digital holography and its application to high-speed 3D imaging of dynamic object

    NASA Astrophysics Data System (ADS)

    Awatsuji, Yasuhiro; Xia, Peng; Wang, Yexin; Matoba, Osamu

    2016-03-01

    Digital holography is a technique of 3D measurement of object. The technique uses an image sensor to record the interference fringe image containing the complex amplitude of object, and numerically reconstructs the complex amplitude by computer. Parallel phase-shifting digital holography is capable of accurate 3D measurement of dynamic object. This is because this technique can reconstruct the complex amplitude of object, on which the undesired images are not superimposed, form a single hologram. The undesired images are the non-diffraction wave and the conjugate image which are associated with holography. In parallel phase-shifting digital holography, a hologram, whose phase of the reference wave is spatially and periodically shifted every other pixel, is recorded to obtain complex amplitude of object by single-shot exposure. The recorded hologram is decomposed into multiple holograms required for phase-shifting digital holography. The complex amplitude of the object is free from the undesired images is reconstructed from the multiple holograms. To validate parallel phase-shifting digital holography, a high-speed parallel phase-shifting digital holography system was constructed. The system consists of a Mach-Zehnder interferometer, a continuous-wave laser, and a high-speed polarization imaging camera. Phase motion picture of dynamic air flow sprayed from a nozzle was recorded at 180,000 frames per second (FPS) have been recorded by the system. Also phase motion picture of dynamic air induced by discharge between two electrodes has been recorded at 1,000,000 FPS, when high voltage was applied between the electrodes.

  19. Shifted knife-edge aperture digital in-line holography for fluid velocimetry.

    PubMed

    Palero, Virginia; Lobera, Julia; Andrés, Nieves; Arroyo, M Pilar

    2014-06-01

    We describe a digital holography technique that, with the simplicity of an in-line configuration, produces holograms where the real and virtual images are completely separated, as in an off-axis configuration. An in-line setup, in which the object is imaged near the sensor, is modified by placing a shifted knife-edge aperture that blocks half the frequency spectrum at the focal plane of the imaging lens. This simple modification of the in-line holographic configuration allows discriminating the virtual and real images. As a fluid velocimetry technique, the use of this aperture removes the minimum defocusing distance requisite and reduces the out-of-plane velocity measurement errors of classical in-line holography. Results with different test objects are shown.

  20. Digital in-line holography on amplitude and phase objects prepared with electron beam lithography.

    PubMed

    Schwenke, J; Lorek, E; Rakowski, R; He, X; Kvennefors, A; Mikkelsen, A; Rudawski, P; Heyl, C M; Maximov, I; Pettersson, S-G; Persson, A; L'Huillier, A

    2012-08-01

    We report on the fabrication and characterization of amplitude and phase samples consisting of well defined Au or Al features formed on ultrathin silicon nitride membranes. The samples were manufactured using electron beam lithography, metallization and a lift-off technique, which allow precise lateral control and thickness of the metal features. The fabricated specimens were evaluated by conventional microscopy, atomic force microscopy and with the digital in-line holography set-up at the Lund Laser Centre. The latter uses high-order harmonic generation as a light source, and is capable of recovering both the shape and phase shifting properties of the samples. We report on the details of the sample production and on the imaging tests with the holography set-up. © 2012 The Authors Journal of Microscopy © 2012 Royal Microscopical Society.

  1. Investigating numerical reconstruction quality and sparsity constraints on the holographic fringe pattern in digital holography

    NASA Astrophysics Data System (ADS)

    Jeong, Hyunwook; Kim, Hak Gu; Ro, Yong Man

    2017-03-01

    In this paper, we investigate quality factors of numerical reconstruction with a small number of signals based on sparsity from a holographic fringe pattern. Holographic fringe pattern generated by Fresnel diffraction is a complex amplitude and sparse distribution in frequency domain. The sparsity of holographic fringe pattern could play a key role in reconstruction quality assessment in compressive holography. In this paper we have investigated sparsity constraint on holographic fringe pattern which influences the overall quality of numerically reconstructed data. In addition, we have investigated reconstruction quality for various subsampling methods including uniform sampling, random sampling, variable density sampling, and magnitude-based sampling. Experiments have been conducted to evaluate reconstruction qualities on sparsity constraints and sampling patterns. Experimental results indicate that the way to extract the sparse signals could significantly affect the quality of the numerical reconstruction in digital holography and visually plausible reconstruction could be obtained with a sparse holographic fringe pattern.

  2. High-speed parallel phase-shifting digital holography system using special-purpose computer for image reconstruction

    NASA Astrophysics Data System (ADS)

    Kakue, Takashi; Shimobaba, Tomoyoshi; Ito, Tomoyoshi

    2015-05-01

    We report a high-speed parallel phase-shifting digital holography system using a special-purpose computer for image reconstruction. Parallel phase-shifting digital holography is a technique capable of single-shot phase-shifting interferometry. This technique records information of multiple phase-shifted holograms required for calculation of phase-shifting interferometry with a single shot by using space-division multiplexing. This technique needs image-reconstruction process for a huge amount of recorded holograms. In particular, it takes a long time to calculate light propagation based on fast Fourier transform in the process and to obtain a motion picture of a dynamically and fast moving object. Then we designed a special-purpose computer for accelerating the image-reconstruction process of parallel phase-shifting digital holography. We developed a special-purpose computer consisting of VC707 evaluation kit (Xilinx Inc.) which is a field programmable gate array board. We also recorded holograms consisting of 128 × 128 pixels at a frame rate of 180,000 frames per second by the constructed parallel phase-shifting digital holography system. By applying the developed computer to the recorded holograms, we confirmed that the designed computer can accelerate the calculation of image-reconstruction process of parallel phase-shifting digital holography ~50 times faster than a CPU.

  3. Study on effects of organic solvents on Hela cells by digital holography

    NASA Astrophysics Data System (ADS)

    Ouyang, Liting; Wang, Dayong; Wang, Yunxin; Wang, Xinlong; Marx, Lisa

    2012-11-01

    In the anticancer research with traditional Chinese medicine, many medicinally effective components can only dissolve in higher polar organic solvents, such as ethanol, dimethyl sulfoxide (DMSO) etc. However, organic solvents may directly interfere with the accuracy of therapeutic efficacy evaluation. Therefore the study on effects of organic solvents with different concentrations on Hela cells is of great significance. The digital holography is a non-destructive and non-contact method to image the transparent sample without staining and with the high precision and high resolution. In this paper, the digital holography is proposed to replace the methyl-thiazol-tetrazolium (MTT) or the Giemsa dye method. Based on the pre-magnification off-axis Fresnel digital holographic theory, an inverted microscopy system is built to obtain the phase-contrast images of the Hela cells, which are added different concentrations of organic solvents. Compared to the control group, there is significantly differences with the shapes of Hela cells with different organic solvents. The size of cell with ethanol 25% is no significantly difference with the control group. But the sizes of cells in the solutions with ethanol 12.5% and 50% are smaller than the control group. Next, the sizes of cells in the solutions with DMSO 12.5%, 25% and 50% are great smaller, compared with the control group. The results show that the digital holography has high practical value in detecting the changes in the shape of cells and is helpful in the choice of organic solvents for further apoptosis study.

  4. Information synthesis (complex amplitude addition and subtraction) and encryption with digital holography and virtual optics.

    PubMed

    Wang, Xiaogang; Zhao, Daomu; Jing, Feng; Wei, Xiaofeng

    2006-02-20

    A new method is proposed in this paper for the synthesis and encryption of information with digital holography technique and virtual optics. By using a three-step phase-shifting interferometry, the fused or subtracted digital hologram can be calculated from different interference patterns. To protect the digital data that can be transmitted through communication channel, an encryption approach based on virtual optics is also proposed. The encryption method proposed is based on extended fractional Fourier transforms. Both the encryption and decryption processes are performed in all-digital manner. The encrypted data and the synthesized data reconstructed numerically also can be stored and transmitted in the conventional communication channel. Numerical simulation results are given to verify the proposed idea.

  5. UV laser interaction with a fluorescent dye solution studied using pulsed digital holography.

    PubMed

    Amer, Eynas; Gren, Per; Sjödahl, Mikael

    2013-10-21

    A frequency tripled Q-switched Nd-YAG laser (wavelength 355 nm, pulse duration 12 ns) has been used to pump Coumarin 153 dye solved in ethanol. Simultaneously, a frequency doubled pulse (532 nm) from the same laser is used to probe the solvent perpendicularly resulting in a gain through stimulated laser induced fluorescence (LIF) emission. The resulting gain of the probe beam is recorded using digital holography by blending it with a reference beam on the detector. Two digital holograms without and with the pump beam were recorded. Intensity maps were calculated from the recorded digital holograms and used to calculate the gain of the probe beam due to the stimulated LIF. In addition numerical data of the local temperature rise was calculated from the corresponding phase maps using Radon inversion. It was concluded that about 15% of the pump beam energy is transferred to the dye solution as heat while the rest is consumed in the radiative process. The results show that pulsed digital holography is a promising technique for quantitative study of fluorescent species.

  6. Application of autofocusing methods in continuous-wave terahertz in-line digital holography

    NASA Astrophysics Data System (ADS)

    Huang, Haochong; Wang, Dayong; Rong, Lu; Zhou, Xun; Li, Zeyu; Wang, Yunxin

    2015-07-01

    Terahertz digital holography is a combination of terahertz imaging and digital holography. During reconstruction, the key point is to find accurately the propagation distance from which the distribution of focused samples can be reconstructed. In this paper, we use a continuous-wave terahertz in-line digital holographic imaging system to record holograms. Moreover, the autofocusing algorithms through which the reconstructed distance can be calculated are applied to the reconstruction. The in-line schematic is beneficial to the terahertz wave imaging, which, however, inevitably produces the object's twin image. In the refocusing process, both the reconstructed image with low signal-to-noise ratio and contrast and the twin image induce the formation of false peaks corresponding to improper distances on the autofocusing curves. To restrain the disturbance factors and improve the accuracy of the judgment, a phase retrieval method is implemented in the reconstruction. The results demonstrate the feasibility of the autofocusing method with phase retrieval in terahertz in-line digital holographic imaging system. The proposed method provides an automated and efficient evaluation which helps to obtain the optimized propagation distance.

  7. A Preliminary Comparison of Three Dimensional Particle Tracking and Sizing using Plenoptic Imaging and Digital In-line Holography

    SciTech Connect

    Guildenbecher, Daniel Robert; Munz, Elise Dahnke; Farias, Paul Abraham; Thurow, Brian S

    2015-12-01

    Digital in-line holography and plenoptic photography are two techniques for single-shot, volumetric measurement of 3D particle fields. Here we present a preliminary comparison of the two methods by applying plenoptic imaging to experimental configurations that have been previously investigated with digital in-line holography. These experiments include the tracking of secondary droplets from the impact of a water drop on a thin film of water and tracking of pellets from a shotgun. Both plenoptic imaging and digital in-line holography successfully quantify the 3D nature of these particle fields. This includes measurement of the 3D particle position, individual particle sizes, and three-component velocity vectors. For the initial processing methods presented here, both techniques give out-of-plane positional accuracy of approximately 1-2 particle diameters. For a fixed image sensor, digital holography achieves higher effective in-plane spatial resolutions. However, collimated and coherent illumination makes holography susceptible to image distortion through index of refraction gradients, as demonstrated in the shotgun experiments. On the other hand, plenotpic imaging allows for a simpler experimental configuration. Furthermore, due to the use of diffuse, white-light illumination, plenoptic imaging is less susceptible to image distortion in the shotgun experiments. Additional work is needed to better quantify sources of uncertainty, particularly in the plenoptic experiments, as well as develop data processing methodologies optimized for the plenoptic measurement.

  8. Multi-parameter motion-picture recording with wide space-bandwidth by parallel phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Tahara, Tatsuki; Xia, Peng; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2014-06-01

    High-speed multicolor 3D motion-picture recording of a 3D object was experimentally demonstrated by using multiwavelength parallel phase-shifting digital holography. Parallel phase-shifting digital holography is a technique for obtaining the complex amplitude distribution of an object wave from a single hologram, based on space-division multiplexing of multiple phase-shifted holograms. The combination of parallel phase-shifting with angular multiplexing is proposed to capture multicolor information simultaneously using a monochromatic image sensor. 3D space, phase, and wavelength information is simultaneously reconstructed by recording a monochromatic hologram using an image sensor with polarization-detection function. Color 3D motion-picture recording of objects that move at the speed of more than 20 km/h was achieved at 20,000 frames per second. This result is the first experimental demonstration of multiwavelength parallel phase-shifting digital holography.

  9. Compressive Holography

    NASA Astrophysics Data System (ADS)

    Lim, Se Hoon

    Compressive holography estimates images from incomplete data by using sparsity priors. Compressive holography combines digital holography and compressive sensing. Digital holography consists of computational image estimation from data captured by an electronic focal plane array. Compressive sensing enables accurate data reconstruction by prior knowledge on desired signal. Computational and optical co-design optimally supports compressive holography in the joint computational and optical domain. This dissertation explores two examples of compressive holography: estimation of 3D tomographic images from 2D data and estimation of images from under sampled apertures. Compressive holography achieves single shot holographic tomography using decompressive inference. In general, 3D image reconstruction suffers from underdetermined measurements with a 2D detector. Specifically, single shot holographic tomography shows the uniqueness problem in the axial direction because the inversion is ill-posed. Compressive sensing alleviates the ill-posed problem by enforcing some sparsity constraints. Holographic tomography is applied for video-rate microscopic imaging and diffuse object imaging. In diffuse object imaging, sparsity priors are not valid in coherent image basis due to speckle. So incoherent image estimation is designed to hold the sparsity in incoherent image basis by support of multiple speckle realizations. High pixel count holography achieves high resolution and wide field-of-view imaging. Coherent aperture synthesis can be one method to increase the aperture size of a detector. Scanning-based synthetic aperture confronts a multivariable global optimization problem due to time-space measurement errors. A hierarchical estimation strategy divides the global problem into multiple local problems with support of computational and optical co-design. Compressive sparse aperture holography can be another method. Compressive sparse sampling collects most of significant field

  10. Hybridization of phase retrieval and off-axis digital holography for high resolution imaging of complex shape objects

    NASA Astrophysics Data System (ADS)

    Wang, Fengpeng; Wang, Dayong; Rong, Lu; Wang, Yunxin; Zhao, Jie

    2017-05-01

    In this paper, a hybrid method of phase retrieval and off-axis digital holography is proposed for imaging of the complex shape objects. Off-axis digital hologram and in-line hologram are recorded. The approximate phase distributions in the recording plane and object plane are obtained by constrained optimization approach from the off-axis hologram, and they are used as the initial value and the constraints in the phase retrieval for eliminating the twin image of in-line holography. Numerical simulations and optical experiments were carried out to validate the proposed method.

  11. Performance comparison of bilinear interpolation, bicubic interpolation, and B-spline interpolation in parallel phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Xia, Peng; Tahara, Tatsuki; Kakue, Takashi; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2013-03-01

    To improve the quality of reconstructed images, we apply bicubic interpolation and B-spline interpolation to parallel phase-shifting digital holography for the first time. The effectiveness of bilinear interpolation, bicubic interpolation, and B-spline interpolation in parallel phase-shifting digital holography is shown by a numerical simulation. In the simulation result, the application of bicubic interpolation and B-spline interpolation succeeded in decreasing the rootmean- square error of the reconstructed image by 12.6 and 11.9%, respectively.

  12. Noninvasive time-dependent cytometry monitoring by digital holography.

    PubMed

    Kemmler, Manuel; Fratz, Markus; Giel, Dominik; Saum, Norbert; Brandenburg, Albrecht; Hoffmann, Christian

    2007-01-01

    Using a digital holographic microscope setup, it is possible to measure dynamic volume changes in living cells. The cells were investigated time-dependently in transmission mode for different kinds of stimuli affecting their morphology. The measured phase shift was correlated to the cellular optical thickness, and then of the cell volume as well as the refractive index were calculated and interpreted. For the characterization of the digital holographic microscope setup, we have developed a transparent three-dimensional (3-D) reference chart that can be used as a lateral resolution chart and step-height resolution chart included in one substrate. For the monitoring of living cells, a biocompatible and autoclavable flow chamber was designed, which allows us to add, exchange, or dilute the fluid within the flow chamber. An integrated changeable coverslip enables inverse microscopic applications. Trypsinization, cell swelling and shrinking induced by osmolarity changes, and apoptosis served as model processes to elucidate the potential of the digital holographic microscopy (DHM).

  13. Fiber-based real-time color digital in-line holography.

    PubMed

    Kowalczyk, Adam; Bieda, Marcin; Makowski, Michal; Sypek, Maciej; Kolodziejczyk, Andrzej

    2013-07-01

    An extremely simple setup for real-time color digital holography using single-mode fibers as light guides and a directional coupler as a beam-splitting device is presented. With the directional coupler we have two object beams and one residual crosstalk used as a reference beam. This facilitates the adjustment and improves robustness. With the use of graphics processing units, real-time hologram reconstruction was possible. Due to adaptation of the optical setup and scaling, zero-order and complex image influence is highly reduced.

  14. Three-dimensional mapping of fluorescent nanoparticles using incoherent digital holography.

    PubMed

    Yanagawa, Takumi; Abe, Ryosuke; Hayasaki, Yoshio

    2015-07-15

    Three-dimensional mapping of fluorescent nanoparticles was performed by using incoherent digital holography. The positions of the nanoparticles were quantitatively determined by using Gaussian fitting of the axial- and lateral-diffraction distributions through position calibration from the observation space to the sample space. It was found that the axial magnification was constant whereas the lateral magnification linearly depended on the axial position of the fluorescent nanoparticles. The mapping of multiple fluorescent nanoparticles fixed in gelatin and a single fluorescent nanoparticle manipulated with optical tweezers in water were demonstrated.

  15. Resolution enhancement in digital holography by self-extrapolation of holograms.

    PubMed

    Latychevskaia, Tatiana; Fink, Hans-Werner

    2013-03-25

    It is generally believed that the resolution in digital holography is limited by the size of the captured holographic record. Here, we present a method to circumvent this limit by self-extrapolating experimental holograms beyond the area that is actually captured. This is done by first padding the surroundings of the hologram and then conducting an iterative reconstruction procedure. The wavefront beyond the experimentally detected area is thus retrieved and the hologram reconstruction shows enhanced resolution. To demonstrate the power of this concept, we apply it to simulated as well as experimental holograms.

  16. Enhancing depth of focus in tilted microfluidics channels by digital holography.

    PubMed

    Matrecano, Marcella; Paturzo, Melania; Finizio, Andrea; Ferraro, Pietro

    2013-03-15

    In this Letter we propose a method to enhance the limited depth of field (DOF) in optical imaging systems, through digital holography. The proposed approach is based on the introduction of a cubic phase plate into the diffraction integral, analogous to what occurs in white-light imaging systems. By this approach we show that it is possible to improve the DOF and to recover the extended focus image of a tilted object in a single reconstruction step. Moreover, we demonstrate the possibility of obtaining well-focused biological cells flowing into a tilted microfluidic channel.

  17. Single-shot self-interference incoherent digital holography using off-axis configuration.

    PubMed

    Hong, Jisoo; Kim, Myung K

    2013-12-01

    We propose a single-shot incoherent holographic imaging technique that adopts self-interference incoherent digital holography (SIDH) with slight tilt of the plane mirror in the optical configuration. The limited temporal coherence length of the illumination leads the guide-star hologram of the proposed system to have a Gaussian envelope of elliptical ring shape. The observation shows that the reconstruction by cross correlation with the guide-star hologram achieves better quality than the usual propagation methods. Experimentally, we verify that the hologram and 3D reconstruction can be implemented incoherently with the proposed single-shot off-axis SIDH.

  18. Feature issue of digital holography and 3D imaging (DH) introduction.

    PubMed

    Hayasaki, Yoshio; Zhou, Changhe; Popescu, Gabriel; Onural, Levent

    2014-11-17

    The OSA Topical Meeting "Digital Holography and 3D Imaging (DH)," was held in Seattle, Washington, July 13-17, 2014. Feature issues based on the DH meeting series have been released by Applied Optics (AO) since 2007. This year Optics Express (OE) and AO jointly decided to have one such feature issue in each journal. The DH meeting will continue in the future, as expected, and the next meeting is scheduled to be held on 24 - 28 May 2015, in Shanghai Institute of Optics and Fine Mechanics, Shanghai, China.

  19. Complex object wave direct extraction method in off-axis digital holography.

    PubMed

    Kim, Daesuk; Magnusson, Robert; Jin, Moonseob; Lee, Jaejong; Chegal, Won

    2013-02-11

    Off-axis digital holography generally uses a 2D-FFT based spatial filtering method to extract the complex object wave from an off-axis hologram. In this paper, we describe a novel single exposure complex object wave extraction method which can provide a faster solution than the FFT based spatial filtering approach while maintaining the reconstructed phase image quality. And also, we show that the proposed direct filtering scheme can provide more robust filtering capability to the off-axis spatial carrier frequency variation than the spatial filtering method.

  20. Developments in digital in-line holography enable validated measurement of 3D particle field dynamics.

    SciTech Connect

    Guildenbecher, Daniel Robert

    2013-12-01

    Digital in-line holography is an optical technique which can be applied to measure the size, three-dimensional position, and three-component velocity of disperse particle fields. This work summarizes recent developments at Sandia National Laboratories focused on improvement in measurement accuracy, experimental validation, and applications to multiphase flows. New routines are presented which reduce the uncertainty in measured position along the optical axis to a fraction of the particle diameter. Furthermore, application to liquid atomization highlights the ability to measure complex, three-dimensional structures. Finally, investigation of particles traveling at near sonic conditions prove accuracy despite significant experimental noise due to shock-waves.

  1. Design of a digital holography system for PFC erosion measurements on Proto-MPEX

    NASA Astrophysics Data System (ADS)

    Thomas, C. E. Tommy; Biewer, T. M.; Baylor, L. R.; Combs, S. K.; Meitner, S. J.; Rapp, J.; Hillis, D. L.; Granstedt, E. M.; Majeski, R.; Kaita, R.

    2016-11-01

    A project has been started at ORNL to develop a dual-wavelength digital holography system for plasma facing component erosion measurements on prototype material plasma exposure experiment. Such a system will allow in situ real-time measurements of component erosion. Initially the system will be developed with one laser, and first experimental laboratory measurements will be made with the single laser system. In the second year of development, a second CO2 laser will be added and measurements with the dual wavelength system will begin. Adding the second wavelength allows measurements at a much longer synthetic wavelength.

  2. Phase imaging of cells by simultaneous dual-wavelength reflection digital holography.

    PubMed

    Khmaladze, Alexander; Kim, Myung; Lo, Chun-Min

    2008-07-21

    We present a phase-imaging technique to quantitatively study the three-dimensional structure of cells. The method, based on the simultaneous dual-wavelength digital holography, allows for higher axial range at which the unambiguous phase imaging can be performed. The technique is capable of nanometer axial resolution. The noise level, which increases as a result of using two wavelengths, is then reduced to the level of a single wavelength. The method compares favorably to software unwrapping, as the technique does not produce non-existent phase steps. Curvature mismatch between the reference and object beams is numerically compensated. The 3D images of SKOV-3 ovarian cancer cells are presented.

  3. Single-shot polarization-imaging digital holography based on simultaneous phase-shifting interferometry.

    PubMed

    Tahara, Tatsuki; Awatsuji, Yasuhiro; Shimozato, Yuki; Kakue, Takashi; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2011-08-15

    We propose single-shot digital holography which is capable of simultaneously capturing both the information of multiple phase-shifted holograms and the distribution of the polarization. In this technique, a single image sensor records both the information required for phase-shifting interferometry and that of the polarization states of objects using an array of polarizers. The essence of the technique is the capability of imaging the distribution of the polarization of three-dimensional objects with a single-shot exposure by using the space-division multiplexing of holograms. The validity of the proposed technique was confirmed by the preliminary experiments. © 2011 Optical Society of America

  4. Phase determination method in statistical generalized phase-shifting digital holography.

    PubMed

    Yoshikawa, Nobukazu

    2013-03-20

    A simple estimation method of the relative phase shift for generalized phase-shifting digital holography based on a statistical method is proposed. This method consists of a selection procedure of an optimum cost function and a simple root-finding procedure. The value and sign of the relative phase shift are determined using the coefficient and the solution of the optimum cost function. The complex field of an object wave is obtained using the estimated relative phase shift. The proposed method lifts the typical restriction on the range of the phase shift due to the phase ambiguity problem. Computer simulations and optical experiments are performed to verify the proposed method.

  5. Color information processing (coding and synthesis) with fractional Fourier transforms and digital holography.

    PubMed

    Chen, Linfei; Zhao, Daomu

    2007-11-26

    In this paper, we propose a new method for color image coding and synthesis based on fractional Fourier transforms and wavelength multiplexing with digital holography. A color image is divided into three channels and each channel, in which the information is encrypted with different wavelength, fractional orders and random phase masks, is independently encrypted or synthesized. The system parameters are additional keys and this method would improve the security of information encryption. The images are fused or subtracted by phase shifting technique. The possible optical implementations for color image encryption and synthesis are also proposed with some simulation results that show the possibility of the proposed idea.

  6. Image reconstruction only by phase data in phase-shifting digital holography.

    PubMed

    Yamaguchi, Ichirou; Yamamoto, Kazuhiro; Mills, Godfrey A; Yokota, Masayuki

    2006-02-10

    We describe data compression in phase-shifting digital holography. We demonstrate by experimentation that an image of a diffusely reflecting object can be reconstructed only by phase data of the derived complex amplitude. It is shown that reduction of the bit depth of the phase data does not seriously damage the image even down to 1 bit. We observe enhancement of halo in the image with low bit depths. This tendency is verified quantitatively by a one-dimensional simulation. Our procedure for smoothing the images that result from the data-compression methods is shown to be effective.

  7. Study of reference waves in single-exposure generalized phase-shifting digital holography.

    PubMed

    Imbe, Masatoshi; Nomura, Takanori

    2013-06-20

    The appropriate reference wave in single-exposure phase-shifting digital holography using a random-complex-amplitude encoded reference wave is experimentally investigated. Although the reference wave is generalized, the quality of reconstructed images depends on it. Furthermore, when the reference wave satisfies a certain condition, reconstructed images cannot be obtained in this method. After the certain condition is presented, the appropriate condition is studied using a speckle property. Experimental results are given to investigate the relations between the quality of reconstructed images and the sizes of speckles of the reference waves. The results prove that the appropriate reference wave exists in this method.

  8. Light-in-Flight Recording by Parallel Phase-Shifting Digital Holography

    NASA Astrophysics Data System (ADS)

    Kakue, Takashi; Yuasa, Junpei; Fujii, Motofumi; Xia, Peng; Tahara, Tatsuki; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2013-09-01

    We propose and demonstrate light-in-flight recording by parallel phase-shifting digital holography to obtain a motion picture of ultrashort light pulse propagation free from unwanted images, which are a zeroth-order diffraction image and a conjugate image, by phase-shifting interferometry with a single-shot exposure. We developed a polarization-imaging camera to record the propagation of a femtosecond light pulse whose center wavelength and temporal duration were 800 nm and 96 fs, respectively, and observed the propagation of the pulse without the unwanted images for 10 ps.

  9. Robust phase-shift estimation method for statistical generalized phase-shifting digital holography.

    PubMed

    Yoshikawa, Nobukazu; Shiratori, Takaaki; Kajihara, Kazuki

    2014-06-16

    We propose a robust phase-shift estimation method for statistical generalized phase-shifting digital holography using a slightly off-axis optical configuration. The phase randomness condition in the Fresnel diffraction field of an object can be sufficiently established by the linear phase factor of the oblique incident reference wave. Signed phase-shift values can be estimated with a statistical approach regardless of the statistical properties of the Fresnel diffraction field of the object. We present computer simulations and optical experiments to verify the proposed method.

  10. Superresolution of interference fringes in parallel four-step phase-shifting digital holography.

    PubMed

    Tahara, Tatsuki; Lee, Yonghee; Ito, Yasunori; Xia, Peng; Shimozato, Yuki; Takahashi, Yuki; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2014-03-15

    A superresolution method for interference fringes obtained by parallel four-step phase-shifting digital holography is proposed. A complex amplitude distribution of an object wave is derived from a recorded hologram by parallel phase-shifting interferometry using two pixels without any interpolation procedures. Multiple distributions are derived by changing one of the two pixels when conducting phase-shifting interferometry. The angular spectrum distribution of the object wave is obtained by both the Fourier transforms and synthesis of the spectrum distribution from the Fourier-transformed images in the spatial frequency domain. Available space bandwidth is extended to half of that of an image sensor.

  11. A new estimation method for two-step-only quadrature phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Zhang, Shuqun; Zhou, Jianyang

    2015-01-01

    Two-step-only quadrature phase-shifting digital holography can reconstruct the original complex object using only two holograms without the additional recording of the reference wave intensity and the object wave intensity. Its success depends on the accurate estimation of the reference wave intensity from the two acquired holograms. The previous estimation method is relatively computational expensive. In this paper, we present a novel simple and effective method for estimating the reference wave intensity with low computational load. Simulation results are presented to demonstrate the effectiveness and speedup of the proposed method.

  12. Quantitative imaging and measurement of cell-substrate surface deformation by digital holography

    NASA Astrophysics Data System (ADS)

    Yu, Xiao; Cross, Michael; Liu, Changgeng; Clark, David C.; Haynie, Donald T.; Kim, Myung K.

    2012-10-01

    Quantitative phase microscopy by digital holography (DH-QPM) is introduced to study the cell-substrate interactions and migratory behavior of adhesive cells. A non-wrinkling elastic substrate, collagen-coated polyacrylamide (PAA) has been employed and its surface deformation due to cell adhesion and motility has been visualized as certain tangential and vertical displacement and distortion. The surface deformation on substrates of different elasticity and thickness has been quantitatively imaged and the corresponding cellular traction force of motile fibroblasts has been measured from phase profiles by DH-QPM. DH-QPM is able to yield quantitative measures directly and provide efficient and versatile means for quantitatively analyzing cellular motility.

  13. Monitoring and evaluation of drying of paint by using phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Yokota, Masayuki

    2010-01-01

    We propose a novel method for monitoring the drying process of a painted surface by using phase-shifting digital holography. In comparison with previous methods using speckle patterns, the proposed method can afford an intensity image for directly monitoring and local variations of drying without an imaging lens. It can also be used for surfaces of complex shapes. In addition, quantitative analysis utilizing a cross-correlation function and phase change derived from the reconstructed complex amplitude is performed and the drying time of paint for different areas and temperature is evaluated. The technique is also applied to monitoring the drying process of a complex surface of a lightbulb.

  14. Three-Dimensional Imaging by Self-Reference Single-Channel Digital Incoherent Holography

    PubMed Central

    Rosen, Joseph; Kelner, Roy

    2016-01-01

    Digital holography offers a reliable and fast method to image a three-dimensional scene from a single perspective. This article reviews recent developments of self-reference single-channel incoherent hologram recorders. Hologram recorders in which both interfering beams, commonly referred to as the signal and the reference beams, originate from the same observed objects are considered as self-reference systems. Moreover, the hologram recorders reviewed herein are configured in a setup of a single channel interferometer. This unique configuration is achieved through the use of one or more spatial light modulators. PMID:28757811

  15. Three-Dimensional Imaging by Self-Reference Single-Channel Digital Incoherent Holography.

    PubMed

    Rosen, Joseph; Kelner, Roy

    2016-08-01

    Digital holography offers a reliable and fast method to image a three-dimensional scene from a single perspective. This article reviews recent developments of self-reference single-channel incoherent hologram recorders. Hologram recorders in which both interfering beams, commonly referred to as the signal and the reference beams, originate from the same observed objects are considered as self-reference systems. Moreover, the hologram recorders reviewed herein are configured in a setup of a single channel interferometer. This unique configuration is achieved through the use of one or more spatial light modulators.

  16. Speckle fluctuation spectroscopy of intracellular motion in living tissue using coherence-domain digital holography

    NASA Astrophysics Data System (ADS)

    Jeong, Kwan; Turek, John J.; Nolte, David D.

    2010-05-01

    Dynamic speckle from 3-D coherence-gated optical sections provides a sensitive label-free measure of cellular activity up to 1 mm deep in living tissue. However, specificity to cellular functionality has not previously been demonstrated. In this work, we perform fluctuation spectroscopy on dynamic light scattering captured using coherence-domain digital holography to obtain the spectral response of tissue that is perturbed by temperature, osmolarity, and antimitotic cytoskeletal drugs. Different perturbations induce specific spectrogram response signatures that can show simultaneous enhancement and suppression in different spectral ranges.

  17. Single-shot self-interference incoherent digital holography using off-axis configuration

    PubMed Central

    Hong, Jisoo; Kim, Myung K.

    2015-01-01

    We propose a single-shot incoherent holographic imaging technique that adopts self-interference incoherent digital holography (SIDH) with slight tilt of the plane mirror in the optical configuration. The limited temporal coherence length of the illumination leads the guide-star hologram of the proposed system to have a Gaussian envelope of elliptical ring shape. The observation shows that the reconstruction by cross correlation with the guide-star hologram achieves better quality than the usual propagation methods. Experimentally, we verify that the hologram and 3D reconstruction can be implemented incoherently with the proposed single-shot off-axis SIDH. PMID:24281544

  18. Speckle fluctuation spectroscopy of intracellular motion in living tissue using coherence-domain digital holography.

    PubMed

    Jeong, Kwan; Turek, John J; Nolte, David D

    2010-01-01

    Dynamic speckle from 3-D coherence-gated optical sections provides a sensitive label-free measure of cellular activity up to 1 mm deep in living tissue. However, specificity to cellular functionality has not previously been demonstrated. In this work, we perform fluctuation spectroscopy on dynamic light scattering captured using coherence-domain digital holography to obtain the spectral response of tissue that is perturbed by temperature, osmolarity, and antimitotic cytoskeletal drugs. Different perturbations induce specific spectrogram response signatures that can show simultaneous enhancement and suppression in different spectral ranges.

  19. Digital in-line holography with a rectangular complex coherence factor.

    PubMed

    Remacha, Clément; Coëtmellec, Sébastien; Brunel, Marc; Lebrun, Denis

    2013-01-01

    We propose in this paper the study of a particular spatially partially coherent source applied to digital in-line holography of dense particle flow. A source with a rectangular complex coherence factor is implemented. The effects of such a source on the intensity distribution of the diffraction pattern are described. In particular, we show that this type of source allows us to eliminate the diffraction pattern along one axis while all the information about the dimension of the particle is kept along the other perpendicular axis. So particle images can be well reconstructed along one direction and the speckle can be largely limited.

  20. Selectable-wavelength low-coherence digital holography with chromatic phase shifter.

    PubMed

    Pham, Quang Duc; Hasegawa, Satoshi; Kiire, Tomohiro; Barada, Daisuke; Yatagai, Toyohiko; Hayasaki, Yoshio

    2012-08-27

    We propose a new digital holography method using an ultra-broadband light source and a chromatic phase-shifter. The chromatic phase-shifter gives different frequency shifts for respective spectral frequencies so that the spectrum of the light reflected from the object can be measured to reveal the spectral property of the object, and arbitrary selection of signals in the temporal frequency domain enables single- and multi-wavelength measurements with wide dynamic range. A theoretical analysis, computer simulations, and optical experiments were performed to verify the advantages of the proposed method.

  1. Fast extended focused imaging in digital holography using a graphics processing unit.

    PubMed

    Wang, Le; Zhao, Jianlin; Di, Jianglei; Jiang, Hongzhen

    2011-05-01

    We present a simple and effective method for reconstructing extended focused images in digital holography using a graphics processing unit (GPU). The Fresnel transform method is simplified by an algorithm named fast Fourier transform pruning with frequency shift. Then the pixel size consistency problem is solved by coordinate transformation and combining the subpixel resampling and the fast Fourier transform pruning with frequency shift. With the assistance of the GPU, we implemented an improved parallel version of this method, which obtained about a 300-500-fold speedup compared with central processing unit codes.

  2. Detection of micromechanical deformation under rigid body displacement using twin-pulsed 3D digital holography

    NASA Astrophysics Data System (ADS)

    Perez-Lopez, Carlos; Hernandez-Montes, Maria del Socorro; Mendoza-Santoyo, Fernando

    2005-02-01

    Twin-pulsed digital holography in its 3D set up is used to recover exclusively the micro-mechanical deformation of an object. The test object is allowed to have rigid body movements such as rotation and translation, with the result that the fringe patterns contain information of the latter and the object deformation, a feature that may significantly modify the interpretation of the results. Experimental results from a flat metal plate subject to micro stress and a displacement in the x-z plane are presented to demonstrate that using this optical method it is possible to recover exclusively the contribution of the micro stress.

  3. Measurement of the thickness of side-polished fiber based on digital holography

    NASA Astrophysics Data System (ADS)

    Wang, Feng; Ma, Jie; Chen, Zhe; Zhong, Jin-gang; Zhong, Yong-chun

    2015-08-01

    A novel method to measure the remaining cladding thickness of side-polished fiber (SPF) was demonstrated by using the digital holography technology. By using angular spectrum method and accurate least-squares phase unwrapping method, the phase distribution of SPF could be reconstructed. Based on the reconstructed phase distribution, the remaining cladding thickness of SPF could be directly measured. It can reduce the errors due to fiber's asymmetries and edge diffraction. This method could also be used to measure other special optical fibers such as photonic crystal fiber and nano-fiber.

  4. Topography of hidden objects using THz digital holography with multi-beam interferences.

    PubMed

    Valzania, Lorenzo; Zolliker, Peter; Hack, Erwin

    2017-05-15

    We present a method for the separation of the signal scattered from an object hidden behind a THz-transparent sample in the framework of THz digital holography in reflection. It combines three images of different interference patterns to retrieve the amplitude and phase distribution of the object beam. Comparison of simulated with experimental images obtained from a metallic resolution target behind a Teflon plate demonstrates that the interference patterns can be described in the simple form of three-beam interference. Holographic reconstructions after the application of the method show a considerable improvement compared to standard reconstructions exclusively based on Fourier transform phase retrieval.

  5. Resolution improvement in off-axis digital holography using an iterative scheme

    NASA Astrophysics Data System (ADS)

    He, Jing; Liu, Cheng; Veetil, Suhas P.; Gao, Shumei; Wang, Jicheng; Wang, Yueke

    2014-08-01

    The image quality in off-axis digital holography (DH) is often degraded by inaccuracies in the reference wave used for reconstruction and the spatial filtering adopted to avoid twin images and zeroth order diffraction. To enhance the image quality in such cases, coherent diffraction imaging is combined with a DH technique to iteratively reconstruct the hologram. By using a small aperture on the sample plane as a spatial constraint and the recorded diffraction pattern as an intensity constraint, a higher spatial resolution than usual is obtained with the proposed method.

  6. In-lab in-line digital holography for cloud particle measurement experiment

    NASA Astrophysics Data System (ADS)

    Li, Huaiqi; Ji, Feng; Li, Liang; Li, Baosheng; Ma, Fei

    2016-10-01

    In terms of climate science, getting the accurate cloud particle sizes, shape and number distributions is necessary for searching the influence of cloud on the environment, radiative transfer, remote sensing measurements and understanding precipitation formation. Many methods and instruments have been developed to measure cloud particles, yet there is still restricted to one-dimensional or two-dimensional projections of particle positions, unable to get the three-dimensional information of the spatial distribution of particles. In-line holography is particularly useful for particles field measurements, because it can directly get the three-dimensional information of the particles and quickly access and storage holographic image. In this paper, the main work is using digital in-line holographic system to measure simulated cloud particles in the laboratory. For digital recording hologram reconstructing, we consider the image intensity in conjunction with the edge sharpness of the particles, to obtain an automatically selected threshold of each particle. Using the threshold, we can get a binary image to identify the particles and separate the particles from background, and then get the information such as the location, shape, particle size of particles. The experimental results show that the in-line digital holography can be used to detect the cloud particles, which can gain many parameters of the simulated cloud particles in the plane perpendicular to the optical axis, and can estimate volume parameters of the simulated cloud particles. This experiment is a basis for the further in situ detection of atmospheric cloud particles.

  7. Stochastic dual-plane on-axis digital holography based on Mach-Zehnder interferometer

    NASA Astrophysics Data System (ADS)

    Wang, Fengpeng; Wang, Dayong; Rong, Lu; Wang, Yunxin; Zhao, Jie

    2016-09-01

    For traditional dual-plane on-axis digital holography, the robustness is lower because it is difficult to maintain the stability of the phase difference between the object beam and the reference beam, and it may be invalid when the objects are on the surface of a medium with uneven thickness. An improved dual-plane digital holographic method based on Mach-Zehnder interferometer is presented to address these problems. Two holograms are recorded at two different planes separated by a small distance. Then, the zero-order image and conjugated image are eliminated by Fourier domain processing. In order to enhance the robustness of the system, the object is illuminated by a stochastic beam that is a speckle wave produced by a diffuser. Simulated and experimental results are shown to demonstrate that the proposed method has greater robustness than the traditional dual-plane on-axis digital holography and it can be used to imaging on the irregular surface of a transparent medium.

  8. Optical scanning holography based on compressive sensing using a digital micro-mirror device

    NASA Astrophysics Data System (ADS)

    A-qian, Sun; Ding-fu, Zhou; Sheng, Yuan; You-jun, Hu; Peng, Zhang; Jian-ming, Yue; xin, Zhou

    2017-02-01

    Optical scanning holography (OSH) is a distinct digital holography technique, which uses a single two-dimensional (2D) scanning process to record the hologram of a three-dimensional (3D) object. Usually, these 2D scanning processes are in the form of mechanical scanning, and the quality of recorded hologram may be affected due to the limitation of mechanical scanning accuracy and unavoidable vibration of stepper motor's start-stop. In this paper, we propose a new framework, which replaces the 2D mechanical scanning mirrors with a Digital Micro-mirror Device (DMD) to modulate the scanning light field, and we call it OSH based on Compressive Sensing (CS) using a digital micro-mirror device (CS-OSH). CS-OSH can reconstruct the hologram of an object through the use of compressive sensing theory, and then restore the image of object itself. Numerical simulation results confirm this new type OSH can get a reconstructed image with favorable visual quality even under the condition of a low sample rate.

  9. Content-based fused off-axis object illumination direct-to-digital holography

    DOEpatents

    Price, Jeffery R.

    2006-05-02

    Systems and methods are described for content-based fused off-axis illumination direct-to-digital holography. A method includes calculating an illumination angle with respect to an optical axis defined by a focusing lens as a function of data representing a Fourier analyzed spatially heterodyne hologram; reflecting a reference beam from a reference mirror at a non-normal angle; reflecting an object beam from an object the object beam incident upon the object at the illumination angle; focusing the reference beam and the object beam at a focal plane of a digital recorder to from the content-based off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis; and digitally recording the content based off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis.

  10. Measurement of three-dimensional deformations using digital holography with radial sensitivity

    SciTech Connect

    Kohler, Christian; Viotti, Matias R.; Albertazzi, Jr.; G. Armando

    2010-07-10

    A measurement system based on digital holography for the simultaneous measurement of out-of-plane and radial in-plane displacement fields for the assessment of residual stress is presented. Two holograms are recorded at the same time with a single image taken by a digital camera, allowing the separate evaluation of in-plane and out-of-plane movement. An axis-symmetrical diffractive optical element is used for the illumination of the object, which causes radial sensitivity vectors. By the addition and, respectively, the subtraction, of the four phase maps calculated from two camera frames, the in-plane and out-of-plane deformation of an object can be calculated separately. The device presented is suitable for high-speed, high-resolution measurement of residual stress. In addition to the setup, first measurement results and a short comparison to a mature digital speckle pattern interferometry setup are shown.

  11. Sensitivity adjustable contouring by digital holography and a virtual reference wavefront

    NASA Astrophysics Data System (ADS)

    Cai, L. Z.; Liu, Q.; Yang, X. L.; Wang, Y. R.

    2003-06-01

    A new method of contouring using digital holography and a virtual reference wavefront is reported. In this method, an object wave is first recorded and then digitally reconstructed. At the same time, a reference wave is digitally introduced to interfere with the reconstructed object wave to form a contour pattern. Since the form or curvature of the reference wave can be arbitrarily designed and artificially generated by a computer, the contouring sensitivity (the depth interval) can be easily adjusted for different purpose. The effectiveness of this method has been verified by computer simulations with both the conventional off-axis hologram and the phase-shifting hologram. The simplicity of optical setup and the unique ability of changing contouring sensitivity in this technique make it attractive potential in practical measurements.

  12. Simultaneous two-dimensional endoscopic pulsed digital holography for evaluation of dynamic displacements.

    PubMed

    Saucedo A, Tonatiuh; Santoyo, Fernando Mendoza; De la Torre Ibarra, Manuel; Pedrini, Giancarlo; Osten, Wolfgang

    2006-07-01

    An endoscope is used in pulsed digital holography to simultaneously evaluate in-plane and out- of-plane transient and harmonic displacements on a flat metallic plate. The plate is illuminated from two different directions. The optical path for each illumination direction is matched to its corresponding reference beam, but also in such a way that each object-reference beam pair optical path is mismatched such that they are incoherent and can be stored in a single CCD frame. As is typical in these types of interferometric arrangement, two digital holograms are needed to compare two different states of the plate. Each hologram is Fourier transformed and due to the incoherence introduced, two separate spectra are readily identified, each belonging to an object-reference beam pair. On comparing by subtraction the phase obtained from the two pulsed digital holograms, it is possible to gather quantitative in-plane and out-of- plane results from transient and harmonic displacements.

  13. Apparatus for direct-to-digital spatially-heterodyned holography

    DOEpatents

    Thomas, Clarence E.; Hanson, Gregory R.

    2006-12-12

    An apparatus operable to record a spatially low-frequency heterodyne hologram including spatially heterodyne fringes for Fourier analysis includes: a laser; a beamsplitter optically coupled to the laser; an object optically coupled to the beamsplitter; a focusing lens optically coupled to both the beamsplitter and the object; a digital recorder optically coupled to the focusing lens; and a computer that performs a Fourier transform, applies a digital filter, and performs an inverse Fourier transform. A reference beam and an object beam are focused by the focusing lens at a focal plane of the digital recorder to form a spatially low-frequency heterodyne hologram including spatially heterodyne fringes for Fourier analysis which is recorded by the digital recorder, and the computer transforms the recorded spatially low-frequency heterodyne hologram including spatially heterodyne fringes and shifts axes in Fourier space to sit on top of a heterodyne carrier frequency defined by an angle between the reference beam and the object beam and cuts off signals around an original origin before performing the inverse Fourier transform.

  14. Sparsity based terahertz reflective off-axis digital holography

    NASA Astrophysics Data System (ADS)

    Wan, Min; Muniraj, Inbarasan; Malallah, Ra'ed; Zhao, Liang; Ryle, James P.; Rong, Lu; Healy, John J.; Wang, Dayong; Sheridan, John T.

    2017-05-01

    Terahertz radiation lies between the microwave and infrared regions in the electromagnetic spectrum. Emitted frequencies range from 0.1 to 10 THz with corresponding wavelengths ranging from 30 μm to 3 mm. In this paper, a continuous-wave Terahertz off-axis digital holographic system is described. A Gaussian fitting method and image normalisation techniques were employed on the recorded hologram to improve the image resolution. A synthesised contrast enhanced hologram is then digitally constructed. Numerical reconstruction is achieved using the angular spectrum method of the filtered off-axis hologram. A sparsity based compression technique is introduced before numerical data reconstruction in order to reduce the dataset required for hologram reconstruction. Results prove that a tiny amount of sparse dataset is sufficient in order to reconstruct the hologram with good image quality.

  15. Multiple-wavelength Color Digital Holography for Monochromatic Image Reconstruction

    NASA Astrophysics Data System (ADS)

    Cheremkhin, P. A.; Shevkunov, I. A.; Petrov, N. V.

    In this paper, we consider the opposite problem, namely, using of color digital holograms simultaneously recorded on several wavelengths for the reconstruction of monochromatic images. Special feature of the procedure of monochromatic image reconstruction from the color hologram is the necessity of extracting information from separate spectral channels with a corresponding overlaying of obtained images to avoid mismatching of their spatial position caused by dependence of methods of numerical reconstruction from the laser wavelength.

  16. Perspectives of multimode fibers and digital holography for optogenetics

    NASA Astrophysics Data System (ADS)

    Czarske, Jürgen W.; Haufe, Daniel; Koukourakis, Nektarios; Büttner, Lars

    2016-04-01

    Optogenetic approaches allow the activation or inhibition of genetically prescribed populations of neurons by light. In principle, optogenetics offers not only the ability to elucidate the functions of neural circuitry, but also new approaches to a treatment of neurodegenerative diseases and recovery of vision and auditory perception. Optogenetics already has revolutionized research in neuroscience. However, new methods for delivering light to three-dimensionally distributed structures e.g. in the brain are necessary. A major hurdle for focusing light through biological tissue is the occurring scattering and scrambling of the light. We demonstrate the correction of the scrambling in a multimode fiber by digital optical phase conjugation with a perspective for optogenetics.

  17. Investigating the Swimming of Microbial Pathogens Using Digital Holography.

    PubMed

    Thornton, K L; Findlay, R C; Walrad, P B; Wilson, L G

    2016-01-01

    To understand much of the behaviour of microbial pathogens, it is necessary to image living cells, their interactions with each other and with host cells. Species such as Escherichia coli are difficult subjects to image: they are typically microscopic, colourless and transparent. Traditional cell visualisation techniques such as fluorescent tagging or phase-contrast microscopy give excellent information on cell behaviour in two dimensions, but no information about cells moving in three dimensions. We review the use of digital holographic microscopy for three-dimensional imaging at high speeds, and demonstrate its use for capturing the shape and swimming behaviour of three important model pathogens: E. coli, Plasmodium spp. and Leishmania spp.

  18. Synthetic aperture single-exposure on-axis digital holography.

    PubMed

    Martínez-León, Lluís; Javidi, Bahram

    2008-01-07

    We present a system for reconstructing single-exposure on-line (SEOL) digital holograms with improved resolution using a synthetic aperture. Several recordings are made in order to compose the synthetic aperture, shifting the camera within the hologram plane. After processing the synthetic hologram, an inverse Fresnel transformation provides an enhanced resolution reconstruction. We show that recognition capacity for high frequency details is increased. Experimental results with a test target and with a microscopic biological sample are presented. Both visualization and correlation results are reported.

  19. Resolution improvement in digital holography by angular and polarization multiplexing.

    PubMed

    Yuan, Caojin; Situ, Guohai; Pedrini, Giancarlo; Ma, Jun; Osten, Wolfgang

    2011-03-01

    Angular and polarization multiplexing techniques are utilized in both object and reference arms in the digital holographic microscopy system to improve its resolution. The angular multiplexing provides on-axis and off-axis illumination and reference beams with different carrier frequencies. Polarization multiplexing prohibits the occurrence of interference between low and high object spatial frequencies and reference beams. The proposed system does not require special light sources or filtering masks. Experimental results show that the resolution of the synthesized image exceeds the resolution determined by the numerical aperture of the imaging microscope objective. © 2010 Optical Society of America

  20. Estimation of vibration frequency of loudspeaker diaphragm by parallel phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Kakue, T.; Endo, Y.; Shimobaba, T.; Ito, T.

    2014-11-01

    We report frequency estimation of loudspeaker diaphragm vibrating at high speed by parallel phase-shifting digital holography which is a technique of single-shot phase-shifting interferometry. This technique records multiple phaseshifted holograms required for phase-shifting interferometry by using space-division multiplexing. We constructed a parallel phase-shifting digital holography system consisting of a high-speed polarization-imaging camera. This camera has a micro-polarizer array which selects four linear polarization axes for 2 × 2 pixels. We set a loudspeaker as an object, and recorded vibration of diaphragm of the loudspeaker by the constructed system. By the constructed system, we demonstrated observation of vibration displacement of loudspeaker diaphragm. In this paper, we aim to estimate vibration frequency of the loudspeaker diaphragm by applying the experimental results to frequency analysis. Holograms consisting of 128 × 128 pixels were recorded at a frame rate of 262,500 frames per second by the camera. A sinusoidal wave was input to the loudspeaker via a phone connector. We observed displacement of the loudspeaker diaphragm vibrating by the system. We also succeeded in estimating vibration frequency of the loudspeaker diaphragm by applying frequency analysis to the experimental results.

  1. Long-working-distance synthetic aperture Fresnel off-axis digital holography.

    PubMed

    Feng, Pan; Wen, Xiao; Lu, Rong

    2009-03-30

    An aperture synthesis approach of digital holography for microscopy imaging at long working distance is proposed. Firstly, for an oblique object, a series of Fresnel off-axis holograms are recorded with different tilted plane wave illuminations without using lens for pre-magnification. Then the complex amplitudes are reconstructed and magnified from these holograms by the double-step Fresnel reconstruction method respectively. Finally, the synthesized image of the resolution enhanced and the speckle suppressed is obtained by incoherent superposition of these reconstructed complex amplitudes. The important advantage of the proposed approach is that the working distance of the system isn't constrained and the reconstructed image doesn't subject to lens aberrations. The experimental results with a die and an USAF-1951 resolution test target are shown and demonstrated that the resolution of both intensity and phase image can be effectively enhanced with simple set-up and procedure. The proposed approach can improve the capabilities of digital holography in three-dimensional in-situ microscopy at long working distance.

  2. Development of a Digital Holography Diagnostic for Surface Characterization at ORNL

    NASA Astrophysics Data System (ADS)

    Biewer, T. M.; Thomas, C. E.

    2016-10-01

    The Fusion and Materials for Nuclear Systems Division (FMNSD) at Oak Ridge National Laboratory (ORNL), in collaboration with Third Dimension Technologies (TDT), proposes to develop a digital holography (DH) surface erosion/deposition diagnostic for imaging 3D plasma facing component (PFC) surfaces in real time. Digital holography is a technique that utilizes IR lasers reflected from a material surface to form a holographic interferogram, which carries information about the topology of the surface when reconstructed. The interrogated surface (at a distance of 3 m) is a region of 2.3 cm x 2.3 cm, and the surface feature resolution is 10 micron or better in depth, around 1 mm transverse to the beam. This is being accomplished in a multi-staged research program at ORNL: 1) establishment of a single-laser DH system ``on the bench,'' 2) establishment of a dual-laser DH system ``on the bench,'' and 3) implementation of the dual-laser DH system on the Proto-MPEX device. The status of the diagnostic development effort will be presented. This work was supported by the US. D.O.E. contract DE-AC05-00OR22725. Research sponsored by the Laboratory Directed Research and Development Program of ORNL, managed by UT Battelle, LLC, for the U.S. D.O.E.

  3. Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography.

    PubMed

    Mölder, A; Sebesta, M; Gustafsson, M; Gisselson, L; Wingren, A Gjörloff; Alm, K

    2008-11-01

    Manual cell counting is time consuming and requires a high degree of skill on behalf of the person performing the count. Here we use a technique that utilizes digital holography, allowing label-free and completely non-invasive cell counting directly in cell culture vessels with adherent viable cells. The images produced can provide both quantitative and qualitative phase information from a single hologram. The recently constructed microscope Holomonitor (Phase Holographic Imaging AB, Lund, Sweden) combines the commonly used phase contrast microscope with digital holography, the latter giving us the possibility of achieving quantitative information on cellular shape, area, confluence and optical thickness. This project aimed at determining the accuracy and repeatability of cell counting measurements using digital holography compared to the conventional manual cell counting method using a haemocytometer. The collected data were also used to determine cell size and cellular optical thickness. The results show that digital holography can be used for non-invasive automatic cell counting as precisely as conventional manual cell counting.

  4. Polarization imaging of a 3D object by use of on-axis phase-shifting digital holography.

    PubMed

    Nomura, Takanori; Javidi, Bahram; Murata, Shinji; Nitanai, Eiji; Numata, Takuhisa

    2007-03-01

    A polarimetric imaging method of a 3D object by use of on-axis phase-shifting digital holography is presented. The polarimetric image results from a combination of two kinds of holographic imaging using orthogonal polarized reference waves. Experimental demonstration of a 3D polarimetric imaging is presented.

  5. Digital holography wavefront sensing in the pupil-plane recording geometry for distributed-volume atmospheric aberrations

    NASA Astrophysics Data System (ADS)

    Banet, Matthias T.; Spencer, Mark F.; Raynor, Robert A.; Marker, Dan K.

    2016-09-01

    Digital holography in the pupil-plane recording geometry shows promise as a wavefront sensor for use in adaptive-optics systems. Because current wavefront sensors suffer from decreased performance in the presence of turbulence and thermal blooming, there is a need for a more robust wavefront sensor in such distributed-volume atmospheric conditions. Digital holography fulfills this roll by accurately estimating the wrapped phase of the complex optical field after propagation through the atmosphere to the pupil plane of an optical system. This paper examines wave-optics simulations of spherical-wave propagation through both turbulence and thermal blooming; it also quantifies the performance of digital holography as a wavefront sensor by generating field-estimated Strehl ratios as a function of the number of pixels in the detector array, the Rytov number, and the Fried coherence diameter. Altogether the results indicate that digital holography wavefront sensing in the pupil-plane recording geometry is a valid and accurate method for estimating the wrapped phase of the complex optical field in the presence of distributed-volume atmospheric aberrations.

  6. Fast particle characterization using digital holography and neural networks.

    PubMed

    Schneider, B; Dambre, J; Bienstman, P

    2016-01-01

    We propose using a neural network approach in conjunction with digital holographic microscopy in order to rapidly determine relevant parameters such as the core and shell diameter of coated, non-absorbing spheres. We do so without requiring a time-consuming reconstruction of the cell image. In contrast to previous approaches, we are able to obtain a continuous value for parameters such as size, as opposed to binning into a discrete number of categories. Also, we are able to separately determine both core and shell diameter. For simulated particle sizes ranging between 7 and 20 μm, we obtain accuracies of (4.4±0.2)% and (0.74±0.01)% for the core and shell diameter, respectively.

  7. Fast focus estimation using frequency analysis in digital holography.

    PubMed

    Oh, Seungtaik; Hwang, Chi-Young; Jeong, Il Kwon; Lee, Sung-Keun; Park, Jae-Hyeung

    2014-11-17

    A novel fast frequency-based method to estimate the focus distance of digital hologram for a single object is proposed. The focus distance is computed by analyzing the distribution of intersections of smoothed-rays. The smoothed-rays are determined by the directions of energy flow which are computed from local spatial frequency spectrum based on the windowed Fourier transform. So our method uses only the intrinsic frequency information of the optical field on the hologram and therefore does not require any sequential numerical reconstructions and focus detection techniques of conventional photography, both of which are the essential parts in previous methods. To show the effectiveness of our method, numerical results and analysis are presented as well.

  8. Measurement of piezoelectric transformer vibrations by digital holography.

    PubMed

    Psota, Pavel; Lédl, Vít; Dolecek, Roman; Erhart, Jirí; Kopecký, Václav

    2012-09-01

    A method for the measurements of the out-of-plane displacement on the surface of vibrating object is presented herein. This method is based on frequency-shifted time-averaged digital holographic interferometry, employing the principle of phase shifting. This approach allows for significant noise reduction, which results in high sensitivity of measurements. This method makes it possible to measure vibrations with amplitudes in the nanometer range over the whole measured surface. This method was applied to the visualization of the out-of-plane vibration modes of piezoelectric transformers. The amplitude and modal shapes were measured with a very high resolution. Furthermore, aspects influencing the measurement errors are discussed and the measurement results by holographic method were compared with the well-established single-point laser interferometry measurement method.

  9. High Speed Digital Holography for Density and Fluctuation Measurements

    SciTech Connect

    ThomasJr., C. E.; Baylor, Larry R; Combs, Stephen Kirk; Meitner, Steven J; Rasmussen, David A; Granstedt, E. M.; Majeski, R.; Kaita, R.

    2010-01-01

    The state of the art in electro-optics has advanced to the point where digital holographic acquisition of wavefronts is now possible. Holographic wavefront acquisition provides the phase of the wavefront at every measurement point. This can be done with accuracy on the order of a thousandth of a wavelength, given that there is sufficient care in the design of the system. At wave frequencies which are much greater than the plasma frequency, the plasma index of refraction is linearly proportional to the electron density and wavelength, and the measurement of the phase of a wavefront passing through the plasma gives the chord-integrated density directly for all points measured on the wavefront. High-speed infrared cameras up to 40 000 fps at 644 pixels with resolutions up to 640512 pixels suitable for use with a CO2 laser are readily available, if expensive.

  10. High speed digital holography for density and fluctuation measurements (invited)

    SciTech Connect

    Thomas, C. E. Jr.; Baylor, L. R.; Combs, S. K.; Meitner, S. J.; Rasmussen, D. A.; Granstedt, E. M.; Majeski, R. P.; Kaita, R.

    2010-10-15

    The state of the art in electro-optics has advanced to the point where digital holographic acquisition of wavefronts is now possible. Holographic wavefront acquisition provides the phase of the wavefront at every measurement point. This can be done with accuracy on the order of a thousandth of a wavelength, given that there is sufficient care in the design of the system. At wave frequencies which are much greater than the plasma frequency, the plasma index of refraction is linearly proportional to the electron density and wavelength, and the measurement of the phase of a wavefront passing through the plasma gives the chord-integrated density directly for all points measured on the wavefront. High-speed infrared cameras (up to {approx}40 000 fps at {approx}64x4 pixels) with resolutions up to 640x512 pixels suitable for use with a CO{sub 2} laser are readily available, if expensive.

  11. High speed digital holography for density and fluctuation measurements (invited).

    PubMed

    Thomas, C E; Baylor, L R; Combs, S K; Meitner, S J; Rasmussen, D A; Granstedt, E M; Majeski, R P; Kaita, R

    2010-10-01

    The state of the art in electro-optics has advanced to the point where digital holographic acquisition of wavefronts is now possible. Holographic wavefront acquisition provides the phase of the wavefront at every measurement point. This can be done with accuracy on the order of a thousandth of a wavelength, given that there is sufficient care in the design of the system. At wave frequencies which are much greater than the plasma frequency, the plasma index of refraction is linearly proportional to the electron density and wavelength, and the measurement of the phase of a wavefront passing through the plasma gives the chord-integrated density directly for all points measured on the wavefront. High-speed infrared cameras (up to ∼40,000 fps at ∼64×4 pixels) with resolutions up to 640×512 pixels suitable for use with a CO(2) laser are readily available, if expensive.

  12. Wavefront sensing for anisotropic turbulence using digital holography

    NASA Astrophysics Data System (ADS)

    Thurman, Samuel T.; Gatt, Philip; Alley, Thomas

    2016-09-01

    We report on digital holographic imaging through atmospheric turbulence. Data recorded with aberrations is corrected during post processing using an iterative sharpness-metric maximization algorithm. Assuming the correction cancels the actual wavefront error, this process is equivalent to wavefront sensing. Much of our past work focused on imaging through isotropic turbulence with phase corrections using a Zernike-polynomial expansion. Here, we describe algorithm modifications for imaging through anisotropic turbulence, similar to what is seen when looking through the aero-optic boundary layer surrounding a moving aircraft. Specifically, we explore tradeoffs associated with switching from a Zernike representation to Karhunen-Loève basis functions. In some cases, the dimensionality of the phase correction estimation algorithm can be reduced significantly by this change. This reduces the computational burden

  13. Imaging through scattering microfluidic channels by digital holography for information recovery in lab on chip.

    PubMed

    Bianco, V; Paturzo, M; Gennari, O; Finizio, A; Ferraro, P

    2013-10-07

    We tackle the problem of information recovery and imaging through scattering microfluidic chips by means of digital holography (DH). In many cases the chip can become opalescent due to residual deposits settling down the inner channel faces, biofilm formation, scattering particle uptake by the channel cladding or its damaging by corrosive substances, or even by condensing effect on the exterior channels walls. In these cases white-light imaging is severely degraded and no information is obtainable at all about the flowing samples. Here we investigate the problem of counting and estimating velocity of cells flowing inside a scattering chip. Moreover we propose and test a method based on the recording of multiple digital holograms to retrieve improved phase-contrast images despite the strong scattering effect. This method helps, thanks to DH, to recover information which, otherwise, would be completely lost.

  14. Effect of intensity quantization level in parallel phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Miao, Lin; Nitta, Kouichi; Matoba, Osamu; Awatsuji, Yasuhiro

    2013-11-01

    Parallel phase-shifting digital holography (PPSDH) enables the instantaneous recording of three-dimensional fields. The three-dimensional field can be reconstructed using a computer by numerical scalar wave propagation. In PPSDH, we record a space-division multiplexed hologram that includes the required phase retardation in the spatial distribution of the reference wave and then interpolate the data at blanked pixels for each phase retardation to obtain the complex amplitude distribution of an object wave. The recorded quality of the multiplexed hologram influences the reconstruction quality. In this study, we investigate the effect of the intensity quantization of a multiplexed hologram on the reconstruction quality. We compare the influence of intensity quantization in PPSDH with that in the conventional phase-shifting method. Random noise is also added to the multiplexed hologram. The required intensity quantization level is helpful for selecting a digital image sensor.

  15. Automatic focusing in digital holography and its application to stretched holograms.

    PubMed

    Memmolo, P; Distante, C; Paturzo, M; Finizio, A; Ferraro, P; Javidi, B

    2011-05-15

    The searching and recovering of the correct reconstruction distance in digital holography (DH) can be a cumbersome and subjective procedure. Here we report on an algorithm for automatically estimating the in-focus image and recovering the correct reconstruction distance for speckle holograms. We have tested the approach in determining the reconstruction distances of stretched digital holograms. Stretching a hologram with a variable elongation parameter makes it possible to change the in-focus distance of the reconstructed image. In this way, the proposed algorithm can be verified at different distances by dispensing the recording of different holograms. Experimental results are shown with the aim of demonstrating the usefulness of the proposed method, and a comparative analysis has been performed with respect to other existing algorithms developed for DH. © 2011 Optical Society of America

  16. Toward soft-tissue elastography using digital holography to monitor surface acoustic waves.

    PubMed

    Li, Shiguang; Mohan, Karan D; Sanders, William W; Oldenburg, Amy L

    2011-11-01

    Measuring the elasticity distribution inside the human body is of great interest because elastic abnormalities can serve as indicators of several diseases. We present a method for mapping elasticity inside soft tissues by imaging surface acoustic waves (SAWs) with digital holographic interferometry. With this method, we show that SAWs are consistent with Rayleigh waves, with velocities proportional to the square root of the elastic modulus greater than 2-40 kPa in homogeneous tissue phantoms. In two-layer phantoms, the SAW velocity transitions approximately from that of the lower layer to that of the upper layer as frequency is increased in agreement with the theoretical relationship between SAW dispersion and the depth-dependent stiffness profile. We also observed deformation in the propagation direction of SAWs above a stiff inclusion placed 8 mm below the surface. These findings demonstrate the potential for quantitative digital holography-based elastography of soft tissues as a noninvasive method for disease detection.

  17. Phase map retrieval in digital holography: avoiding the undersampling effect by a lateral shear approach.

    PubMed

    Ferraro, P; Del Core, C; Miccio, L; Grilli, S; De Nicola, S; Finizio, A; Coppola, G

    2007-08-01

    In digital holography (DH) the numerical reconstruction of the whole wavefront allows one to extract the wrapped phase map mod, 2 pi. It can occur that the reconstructed wrapped phase map in the image plane is undersampled because of the limited pixel size in that plane. In such a case the phase distribution cannot be retrieved correctly by the usual unwrapping procedures. We show that the use of the digital lateral-shearing interferometry approach in DH provides the correct reconstruction of the phase map in the image plane, even in extreme cases where the phase profile changes very rapidly. We demonstrate the effectiveness of the method in a particular case where the profile of a highly curved silicon microelectromechanical system membrane has to be reconstructed.

  18. Continuous-wave terahertz multi-plane in-line digital holography

    NASA Astrophysics Data System (ADS)

    Huang, Haochong; Wang, Dayong; Li, Weihua; Rong, Lu; Taylor, Zachary D.; Deng, Qinghua; Li, Bin; Wang, Yunxin; Wu, Weidong; Panezai, Spozmai

    2017-07-01

    Terahertz digital holography is a non-scanning and real time method for reconstructing the absorption and phase distributions of the wave-front diffracted by a given sample simultaneously in the terahertz region. A continuous-wave terahertz in-line digital holographic multi-plane imaging method is presented here for achieving a three-dimensional shape of a specific portion of a sample with the best possible focus. The three enhancement techniques of synthetic aperture, autofocusing and phase retrieval are applied to the single plane recordings for achieving a high resolution, good quality and optimally focused reconstructed image. Later, multi-plane reconstructed images are processed with the threshold mask and a three dimensional profile of the sample is obtained. Experimental verification confirms that the proposed method is a valid tool for acquiring multi-plane information of a target in the terahertz range.

  19. Optical implementation of cipher block chaining mode algorithm using phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Jeon, Seok-Hee; Gil, Sang-Keun

    2016-12-01

    We propose an optical design of cipher block chaining (CBC) encryption mode using digital holography, which is implemented by the two-step quadrature phase-shifting digital holographic encryption technique using orthogonal polarization. A block of plain text is encrypted with the encryption key by applying the two-step phase-shifting digital holographic method; then, it is changed into cipher text blocks which are digital holograms. Optically, these digital holograms with the encrypted information are Fourier transform holograms and are recorded onto charge-coupled devices with 256 quantization gray levels. This means that the proposed optical CBC encryption is a scheme that has an analog-type of pseudorandom pattern information in the cipher text, while the conventional electronic CBC encryption is a kind of bitwise block message encryption processed by digital bits. Also, the proposed method enables the cryptosystem to have higher security strength and faster processing than the conventional electronic method because of the large two-dimensional (2-D) array key space and parallel processing. The results of computer simulations verify that the proposed optical CBC encryption design is very effective in CBC mode due to fast and secure optical encryption of 2-D data and shows the feasibility for the CBC encryption mode.

  20. Multiwavelength digital holography for polishing tool shape measurement

    NASA Astrophysics Data System (ADS)

    Lédl, Vít.; Psota, Pavel; Václavík, Jan; Doleček, Roman; Vojtíšek, Petr

    2013-09-01

    Classical mechano-chemical polishing is still a valuable technique, which gives unbeatable results for some types of optical surfaces. For example, optics for high power lasers requires minimized subsurface damage, very high cosmetic quality, and low mid spatial frequency error. One can hardly achieve this with use of subaperture polishing. The shape of the polishing tool plays a crucial role in achieving the required form of the optical surface. Often the shape of the polishing tool or pad is not known precisely enough during the manufacturing process. The tool shape is usually premachined and later is changed during the polishing procedure. An experienced worker could estimate the shape of the tool indirectly from the shape of the polished element, and that is why he can achieve the required shape in few reasonably long iterative steps. Therefore the lack of the exact tool shape knowledge is tolerated. Sometimes, this indirect method is not feasible even if small parts are considered. Moreover, if processes on machines like planetary (continuous) polishers are considered, the incorrect shape of the polishing pad could extend the polishing times extremely. Every iteration step takes hours. Even worse, polished piece could be wasted if the pad has a poor shape. The ability of the tool shape determination would be very valuable in those types of lengthy processes. It was our primary motivation to develop a contactless measurement method for large diffusive surfaces and demonstrate its usability. The proposed method is based on application of multiwavelength digital holographic interferometry with phase shift.

  1. Digital polarization holography advancing 4G optics (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    De Sio, Luciano; Roberts, David E.; Tabiryan, Nelson V.; Steeves, Diane M.; Kimball, Brian R.

    2016-09-01

    The fourth generation optics (4G optics) enables the realization of novel optical components (lenses, gratings, vector vortices, etc.) by patterning the optical axis orientation in the plane of an anisotropic film. Such components exhibit near 100% diffraction efficiency for wavelengths meeting half-wave retardation condition. In this framework, we have advanced a step-forward by realizing different diffractive waveplates (DWs) with arbitrary spatial patterns of the optical axis orientation by exploiting the capability of a Digital Spatial Light Polarization Converter (DSLPC). The DSLPC is based on a reflective, high resolution Spatial Light Modulator (SLM) combined with an "ad hoc" optical setup. The most attractive feature of the use of a DSLPC for photoalignment is that the orientation of the alignment layer, and therefore of the fabricated liquid crystal (LC) or liquid crystal polymer (LCP) DWs, can be specified on a pixel-by-pixel basis. By varying the optical magnification or de-magnification between the SLM and the alignment layer, the spatial resolution of the photoaligned layer can be adjusted to be optimal for each application. We show that with a simple "click" it is possible to record different high resolution optical components as well as arbitrary patterns ranging from lenses to invisible and even dual labels.

  2. Design of the experimental system of digital off-axis holography and analysis of zero-order image

    NASA Astrophysics Data System (ADS)

    L', Qieni; Ge, Baozhen; Jiang, Junfeng; Zhang, Yimo

    2005-02-01

    The simple hologram recording system of digital off-axis holography is designed. Holographic diffraction grating of low spatial frequency is introduced to digital holography, acting as a beam splitter, illuminated by the monochrome plane parallel light wave, and the three different plane parallel light waves, namely, the zero-order and the +/-1order diffraction waves, are generated. An off-axis holography system is configurated between the +1/-1order diffraction waves and the zero-order wave. The theoretical analysis has been done in detail and the zero-order diffraction in the reconstructed image of digital off-axis holography is also analyzed theoretically. The experimental results are given. The feasibility of the experimental system presented in this paper was confirmed, simple in optical structure and easy in operation. In addition, the zero-order image can be eliminated by numerically processing the holograms of object with different recording parameters obtained by adjusting the reflecting mirror in the recording optical system to vary the incidence beam orientation in CCD to introduce a phase shift.

  3. Micro-light-pipe array with an excitation attenuation filter for lensless digital enzyme-linked immunosorbent assay

    NASA Astrophysics Data System (ADS)

    Takehara, Hironari; Nagasaki, Mizuki; Sasagawa, Kiyotaka; Takehara, Hiroaki; Noda, Toshihiko; Tokuda, Takashi; Ohta, Jun

    2016-03-01

    Digital enzyme-linked immunosorbent assay (ELISA) is used for detecting various biomarkers with hypersensitivity. We have been developing compact systems by replacing the fluorescence microscope with a CMOS image sensor. Here, we propose a micro-light-pipe array structure made of metal filled with dye-doped resin, which can be used as a fabrication substrate of the micro-reaction-chamber array of digital ELISA. The possibility that this structure enhances the coupling efficiency for fluorescence was simulated using a simple model. To realize the structure, we fabricated a 30-µm-thick micropipe array by copper electroplating around a thick photoresist pattern. The typical diameter of each fabricated micropipe was 10 µm. The pipes were filled with yellow-dye-doped epoxy resin. The transmittance ratio of fluorescence and excitation light could be controlled by adjusting the doping concentration. We confirmed that an angled excitation light incidence suppressed the leakage of excitation light.

  4. Estimation of objects transverse parameters in off-axis and in-line Fresnel digital holography

    NASA Astrophysics Data System (ADS)

    Cheremkhin, Pavel A.; Evtikhiev, Nikolay N.; Krasnov, Vitaly V.; Rodin, Vladislav G.; Starikov, Sergey N.

    2015-05-01

    In this report transverse parameters of objects registered with inline and off-axis Fresnel digital holography schemes were estimated: maximum transverse dimensions of objects, size and quantity of object resolution elements. By determining allowed locations of diffraction orders under reconstruction, new expressions for estimation of objects transverse parameters were obtained. In case of off-axis holography it is desirable that object should not overlap with zero-order and twin images. If object and twin images are located on opposite sides relative to zero-order under reconstruction, this is case of preventing of cyclic shift of twin image (PCS). If twin image is located on both sides relative to zero-order under reconstruction but don't overlap with object image, this is case of assumption of cyclic shift of twin image (ACS). ACS case allows to register digital holograms of larger objects compared to PCS case. However, for example, for automatic image processing, separate display of object and twin images relative to zero-order is often required. It was found that ACS case allows to register holograms with distance between the object and hologram 1.5 times lesser than in PCS case. And maximum transverse dimension of object in ACS case is always greater than in PCS case by the half of hologram size. For verification of obtained estimates, off-axis digital Fresnel holograms with 2048x2048 pixels were optically registered. Contour images located behind static scatter were used as objects. Confirmations on transverse object parameters estimates, satisfying ACS and PCS cases, were derived. These results demonstrate correctness of obtained quantitative estimates.

  5. Remote monitoring of building oscillation modes by means of real-time Mid Infrared Digital Holography

    PubMed Central

    Poggi, Pasquale; Locatelli, Massimiliano; Pugliese, Eugenio; Delle Donne, Dario; Lacanna, Giorgio; Meucci, Riccardo; Ripepe, Maurizio

    2016-01-01

    Non-destructive measurements of deformations are a quite common application of holography but due to the intrinsic limits in the interferometric technique, those are generally confined only to small targets and in controlled environment. Here we present an advanced technique, based on Mid Infrared Digital Holography (MIR DH), which works in outdoor conditions and provides remote and real-time information on the oscillation modes of large engineering structures. Thanks to the long wavelength of the laser radiation, large areas of buildings can be simultaneously mapped with sub-micrometric resolution in terms of their amplitude and frequency oscillation modes providing all the modal parameters vital for all the correct prevention strategies when the functionality and the health status of the structures have to be evaluated. The existing experimental techniques used to evaluate the fundamental modes of a structure are based either on seismometric sensors or on Ground-based Synthetic Aperture Radar (GbSAR). Such devices have both serious drawbacks, which prevent their application at a large scale or in the short term. We here demonstrate that the MIR DH based technique can fully overcome these limitations and has the potential to represent a breakthrough advance in the field of dynamic characterization of large structures. PMID:27032810

  6. Digital holography on moving objects: multiwavelength height measurements on inclined surfaces

    NASA Astrophysics Data System (ADS)

    Schiller, Annelie; Beckmann, Tobias; Fratz, Markus; Belzer, Dominik; Bertz, Alexander; Carl, Daniel; Buse, Karsten

    2017-06-01

    Multiwavelength digital holography on moving objects enables fast and precise inline-measurements of surface pro files. Due to the use of multiple wavelengths, optically rough surfaces with structure heights in the micrometer range can be mapped unambiguously. In this work we explore the influence of the object velocity on height measurements on inclined surfaces. We show measurements using spatial-phase-shifting holography employing two wavelengths and object velocities of up to 90 mm/s with eye-safe cw-lasers with less than 1 mW of laser light. Despite motion blur exceeding the mean speckle size, reliable height measurements can be conducted at these velocities. The height map of a metal cone with two different slope angles (1° , 10° ) is measured at an exposure time of 2 ms. Using line shaped illumination, each frame yields a height map of approximately 2 x 17 mm2. The overlap between the frames allows averaging as the image is put together, improving data quality. The mean repeatability of the height information in the investigated setup is better than 4.5 µm at a synthetic wavelength of 214 µm.

  7. Remote monitoring of building oscillation modes by means of real-time Mid Infrared Digital Holography

    NASA Astrophysics Data System (ADS)

    Poggi, Pasquale; Locatelli, Massimiliano; Pugliese, Eugenio; Delle Donne, Dario; Lacanna, Giorgio; Meucci, Riccardo; Ripepe, Maurizio

    2016-04-01

    Non-destructive measurements of deformations are a quite common application of holography but due to the intrinsic limits in the interferometric technique, those are generally confined only to small targets and in controlled environment. Here we present an advanced technique, based on Mid Infrared Digital Holography (MIR DH), which works in outdoor conditions and provides remote and real-time information on the oscillation modes of large engineering structures. Thanks to the long wavelength of the laser radiation, large areas of buildings can be simultaneously mapped with sub-micrometric resolution in terms of their amplitude and frequency oscillation modes providing all the modal parameters vital for all the correct prevention strategies when the functionality and the health status of the structures have to be evaluated. The existing experimental techniques used to evaluate the fundamental modes of a structure are based either on seismometric sensors or on Ground-based Synthetic Aperture Radar (GbSAR). Such devices have both serious drawbacks, which prevent their application at a large scale or in the short term. We here demonstrate that the MIR DH based technique can fully overcome these limitations and has the potential to represent a breakthrough advance in the field of dynamic characterization of large structures.

  8. Depth of focus in digital holography using spatial partially coherent light

    NASA Astrophysics Data System (ADS)

    Binz, Kristen; Yu, Xiao; Clark, David; Kim, Myung K.

    2012-02-01

    Digital holography is a powerful, but young, imaging technology that has a vast array of applications; its strength lies in the ability to numerically focus on any plane within a sample from a single hologram and to use both amplitude and phase information from the intensity field to reconstruct the sample's 3D characteristics on a computer. The quality of many holograms, however, is compromised by speckle and other interference noise associated with the high-coherence lasers often used to illuminate the sample. Speckle noise may be diminished by lowering the coherence length of the source. In our experiments, partially coherent light was created by directing a laser beam through a rotating ground glass. We aimed to discern whether the coherence of the source could be systematically altered by changing the position of the ground glass within the focused laser beam. We anticipated that altering the coherence length would also systematically change the depth of focus. Initial results support our hypotheses.

  9. Quality investigation of surface mount technology using phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Boonsri, Chantira; Buranasiri, Prathan

    2016-09-01

    Applying of a phase-shifting digital holography combined with compressive sensing to inspect the soldering quality of surface mount technology (SMT) which is a method for producing electronic circuits. In SMT, the components are mounted and connected with each other directly onto the surface of printed circuit boards (PCBs). By reconstructing the multidimensional images from a few samples of SMT, the results are solved by an optimization problem. In this paper, two problems have been concerned. The first one is to examine the devices and the soldering quality of connections between them, which are in micro-scaled. The second is to observe the effect of heat treatment of soldering material and devices on the surface mount board.

  10. Four-dimensional tracking of spatially incoherent illuminated samples using self-interference digital holography

    NASA Astrophysics Data System (ADS)

    Man, Tianlong; Wan, Yuhong; Wu, Fan; Wang, Dayong

    2015-11-01

    We present a new method for the four-dimensional tracking of a spatially incoherent illuminated object. Self-interference digital holography is utilized for recording the hologram of the spatially incoherent illuminated object. Three-dimensional spatial coordinates encoded in the hologram are extracted by holographic reconstruction procedure and tracking algorithms, while the time information is reserved by the single-shot configuration. Applications of the holographic tracking methods are expanded to the incoherent imaging areas. Speckles and potential damage to the samples of the coherent illuminated tracking methods are overcome. Results on the quantitative tracking of three-dimensional spatial position over time are reported. In practical, living zebra fish larva is used to demonstrate one of the applications of the method.

  11. Quantitative, three-dimensional diagnostics of multiphase drop fragmentation via digital in-line holography.

    PubMed

    Gao, Jian; Guildenbecher, Daniel R; Reu, Phillip L; Kulkarni, Varun; Sojka, Paul E; Chen, Jun

    2013-06-01

    Quantitative application of digital in-line holography (DIH) to characterize multiphase fragmentation is demonstrated. DIH is applied to record sequential holograms of the breakup of an ethanol droplet in an aerodynamic flow field. Various stages of the breakup process are recorded, including deformation, bag growth, bag breakup, and rim breakup. A recently proposed hybrid method is applied to extract the three-dimensional (3D) location and size of secondary droplets as well as the 3D morphology of the rim. Particle matching between sequential frames is used to determine the velocity. Coincidence with the results obtained from phase Doppler anemometry measurement demonstrates the accuracy of measurement by DIH and the hybrid method.

  12. Sparsity-Based Pixel Super Resolution for Lens-Free Digital In-line Holography.

    PubMed

    Song, Jun; Leon Swisher, Christine; Im, Hyungsoon; Jeong, Sangmoo; Pathania, Divya; Iwamoto, Yoshiko; Pivovarov, Misha; Weissleder, Ralph; Lee, Hakho

    2016-04-21

    Lens-free digital in-line holography (LDIH) is a promising technology for portable, wide field-of-view imaging. Its resolution, however, is limited by the inherent pixel size of an imaging device. Here we present a new computational approach to achieve sub-pixel resolution for LDIH. The developed method is a sparsity-based reconstruction with the capability to handle the non-linear nature of LDIH. We systematically characterized the algorithm through simulation and LDIH imaging studies. The method achieved the spatial resolution down to one-third of the pixel size, while requiring only single-frame imaging without any hardware modifications. This new approach can be used as a general framework to enhance the resolution in nonlinear holographic systems.

  13. Uncertainty characterization of particle depth measurement using digital in-line holography and the hybrid method.

    PubMed

    Gao, Jian; Guildenbecher, Daniel R; Reu, Phillip L; Chen, Jun

    2013-11-04

    In the detection of particles using digital in-line holography, measurement accuracy is substantially influenced by the hologram processing method. In particular, a number of methods have been proposed to determine the out-of-plane particle depth (z location). However, due to the lack of consistent uncertainty characterization, it has been unclear which method is best suited to a given measurement problem. In this work, depth determination accuracies of seven particle detection methods, including a recently proposed hybrid method, are systematically investigated in terms of relative depth measurement errors and uncertainties. Both synthetic and experimental holograms of particle fields are considered at conditions relevant to particle sizing and tracking. While all methods display a range of particle conditions where they are most accurate, in general the hybrid method is shown to be the most robust with depth uncertainty less than twice the particle diameter over a wide range of particle field conditions.

  14. Digital holography super-resolution for accurate three-dimensional reconstruction of particle holograms.

    PubMed

    Verrier, Nicolas; Fournier, Corinne

    2015-01-15

    In-line digital holography (DH) is used in many fields to locate and size micro or nano-objects spread in a volume. To reconstruct simple shaped objects, the optimal approach is to fit an imaging model to accurately estimate their position and their characteristic parameters. Increasing the accuracy of the reconstruction is a big issue in DH, particularly when the pixel is large or the signal-to-noise ratio is low. We suggest exploiting the information redundancy of videos to improve the reconstruction of the holograms by jointly estimating the position of the objects and the characteristic parameters. Using synthetic and experimental data, we checked experimentally that this approach can improve the accuracy of the reconstruction by a factor more than the square root of the image number.

  15. Digital in-line X-ray holography with zone plates.

    PubMed

    Heine, R; Gorniak, T; Nisius, T; Christophis, C; Pettitt, M E; Staier, F; Wilhein, T; Rehbein, S; Grunze, M; Rosenhahn, A

    2011-07-01

    Single pulse imaging with radiation provided by free-electron laser sources is a promising approach towards X-ray microscopy, which is expected to provide high resolution images of biological samples unaffected by radiation damage. One fully coherent imaging technique for this purpose is digital in-line holography. Key to its successful application is the creation of X-ray point sources with high photon flux. In this study we applied zone plates to create such point sources with synchrotron radiation provided by the storage ring BESSY II. The obtained, divergent light cone is applied to holographic microscopy of biological objects such as critical point dried Navicula perminuta diatoms and human cells using photons with an energy of 250 eV. Compared to conventional experiments employing pinholes, exposure times are reduced by two orders of magnitude.

  16. Motion compensation and noise tolerance in phase-shifting digital in-line holography.

    PubMed

    Stenner, Michael D; Neifeld, Mark A

    2006-05-15

    We present a technique for phase-shifting digital in-line holography which compensates for lateral object motion. By collecting two frames of interference between object and reference fields with identical reference phase, one can estimate the lateral motion that occurred between frames using the cross-correlation. We also describe a very general linear framework for phase-shifting holographic reconstruction which minimizes additive white Gaussian noise (AWGN) for an arbitrary set of reference field amplitudes and phases. We analyze the technique's sensitivity to noise (AWGN, quantization, and shot), errors in the reference fields, errors in motion estimation, resolution, and depth of field. We also present experimental motion-compensated images achieving the expected resolution.

  17. Single-shot dual-wavelength phase unwrapping in parallel phase-shifting digital holography.

    PubMed

    Lee, Yonghee; Ito, Yasunori; Tahara, Tatsuki; Inoue, Junichi; Xia, Peng; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Matoba, Osamu

    2014-04-15

    We propose a single-shot phase-unwrapping method using two wavelengths in parallel phase-shifting digital holography (PPSDH). The proposed method enables one to solve the phase ambiguity problem in PPSDH. We conducted an experiment of the proposed method using two lasers whose wavelengths are 473 and 532 nm. An object having about 1.9 μm step, which is 7.1 times larger than the half wavelength of one of the lasers (266 nm), was fabricated by using vapor deposition of aluminum. Single-shot measurement of the height of the object was successfully demonstrated, and the validity of the proposed method was verified.

  18. Magnetic-flux quanta in superconducting thin films observed by electron holography and digital phase analysis

    SciTech Connect

    Hasegawa, S.; Matsuda, T.; Endo, J.; Osakabe, N.; Igarashi, M.; Kobayashi, T.; Naito, M.; Tonomura, A. ); Aoki, R. )

    1991-04-01

    Singly quantized magnetic fluxes in superconducting lead films have been directly observed in the form of magnetic-flux-line distributions by using an electron-holography technique. Combining this with the digital-phase-analysis method, we were able to determine the flux quantum {ital h}/2{ital e} for individual fluxes with a precision of {similar to}{ital h}/100{ital e}, and analyze the distributions of field-vector components around the fluxon centers. The internal-field distributions obtained were compared with those calculated from the Ginzburg-Landau equations with use of some models, and an overall agreement was found between them. We also observed the changes of the magnetic-flux structures of lead thin films as a function of their thickness. Fluxon pairs were observed in 0.2-{mu}m-thick films, which may correspond to those suggested by Kosterlitz-Thouless theory.

  19. Incoherent digital holography system utilizing single-shot phase-shifting interferometry

    NASA Astrophysics Data System (ADS)

    Tahara, Tatsuki; Kanno, Takeya; Arai, Yasuhiko; Ozawa, Takeaki

    2017-04-01

    We propose single-shot incoherent digital holography with a common-path configuration and single-shot four-step phase-shifting interferometry and construct a system based on the proposal. Space-division multiplexing and states of the polarizations of the waves are utilized to implement single-shot phase-shifting interferometry. A common-path setup is constructed to capture an incoherent hologram easily with a compact system. An instantaneous and three-dimensional object image is obtained without undesired diffraction waves by phase-shifting interferometry from a single recorded image. The validity of the proposed technique is experimentally investigated for an object with a diameter of 1 mm.

  20. Assessment of weak light condition in parallel four-step phase-shifting digital holography.

    PubMed

    Miao, Lin; Nitta, Kouichi; Matoba, Osamu; Awatsuji, Yasuhiro

    2013-01-01

    Minimum optical energy required for four-step parallel phase-shifting digital holography (PPSDH) is evaluated numerically by using photon-counting analysis. PPSDH enables us to develop instantaneous three-dimensional (3D) measurement by single-shot measurement. In fast measurement of dynamic 3D events, detected optical power at an image sensor will be decreased. For biomedical sensing, maximum light intensity exists for preventing the damage of the tissue. In the numerical evaluation, a photon-counting approach is used for the evaluation of minimum detected energy by comparing the reconstructed images. Numerical results indicate that hundreds of photons at each pixel in the image sensor are enough for the reconstruction and total detected energy in a multiplexed hologram is about 1 pJ.

  1. Multiwavelength digital holography with wavelength-multiplexed holograms and arbitrary symmetric phase shifts.

    PubMed

    Tahara, Tatsuki; Otani, Reo; Omae, Kaito; Gotohda, Takuya; Arai, Yasuhiko; Takaki, Yasuhiro

    2017-05-15

    We propose multiwavelength in-line digital holography with wavelength-multiplexed phase-shifted holograms and arbitrary symmetric phase shifts. We use phase-shifting interferometry selectively extracting wavelength information to reconstruct multiwavelength object waves separately from wavelength-multiplexed monochromatic images. The proposed technique obtains systems of equations for real and imaginary parts of multiwavelength object waves from the holograms by introducing arbitrary symmetric phase shifts. Then, the technique derives each complex amplitude distribution of each object wave selectively and analytically by solving the two systems of equations. We formulate the algorithm in the case of an arbitrary number of wavelengths and confirm its validity numerically and experimentally in the cases where the number of wavelengths is two and three.

  2. Image formation in phase-shifting digital holography and applications to microscopy.

    PubMed

    Yamaguchi, I; Kato, J; Ohta, S; Mizuno, J

    2001-12-01

    We discuss image formation in phase-shifting digital holography by developing an analytical formulation based on the Fresnel-Kirchhoff diffraction theory. Image-plane position and imaging magnification are derived for general configurations in which a spherical reference is employed. The influences of discrete sampling of the resulting interference patterns by a CCD and numerical reconstruction on qualities of point images are investigated. Dependence of the point images on the ratio of the minimum fringe spacing to pixel pitch of the CCD is numerically analyzed. Two-point resolution and magnification are also investigated as a function of pixel numbers by a simulation using a one-dimensional model. In experiments magnified images of biological objects and a resolution target were reconstructed with the same quality as by conventional microscopy.

  3. Large object investigation by digital holography with effective spectrum multiplexing under single-exposure approach

    SciTech Connect

    Liu, Ning Zhang, Yingying; Xie, Jun

    2014-10-13

    We present a method to investigate large object by digital holography with effective spectrum multiplexing under single-exposure approach. This method splits the original reference beam and redirects one of its branches as a second object beam. Through the modified Mach-Zehnder interferometer, the two object beams can illuminate different parts of the large object and create a spectrum multiplexed hologram onto the focal plane array of the charge-coupled device/complementary metal oxide semiconductor camera. After correct spectrum extraction and image reconstruction, the large object can be fully observed within only one single snap-shot. The flexibility and great performance make our method a very attractive and promising technique for large object investigation under common 632.8 nm illumination.

  4. Design of a digital holography system for PFC erosion measurements on Proto-MPEX

    SciTech Connect

    ThomasJr., C. E.; Biewer, Theodore M; Baylor, Larry R; Combs, Stephen Kirk; Meitner, Steven J; Rapp, Juergen; Hillis, Donald Lee; Granstedt, E. M.; Majeski, R.; Kaita, R.

    2016-01-01

    A project has been started at ORNL to develop a dual-wavelength digital holography system for plasma facing component (PFC) erosion measurements on Proto-MPEX. Such a system will allow in-situ real-time measurements of component erosion. Initially the system will be developed with one laser, and first experimental laboratory measurements will be made with the single laser system. In the second year of development a second CO2 laser will be added and measurements with the dual wavelength system will begin. Adding the second wavelength allows measurements at a much longer synthetic wavelength equal to the average of the two wavelengths times the average divided by the difference of the two wavelengths.

  5. Investigation of 3D surface acoustic waves in granular media with 3-color digital holography

    NASA Astrophysics Data System (ADS)

    Leclercq, Mathieu; Picart, Pascal; Penelet, Guillaume; Tournat, Vincent

    2017-01-01

    This paper reports the implementation of digital color holography to investigate elastic waves propagating along a layer of a granular medium. The holographic set-up provides simultaneous recording and measurement of the 3D dynamic displacement at the surface. Full-field measurements of the acoustic amplitude and phase at different excitation frequencies are obtained. It is shown that the experimental data can be used to obtain the dispersion curve of the modes propagating in this granular medium layer. The experimental dispersion curve and that obtained from a finite element modeling of the problem are found to be in good agreement. In addition, full-field images of the interaction of an acoustic wave guided in the granular layer with a buried object are also shown.

  6. Detection of inhomogeneities in semi-solid materials using pulsed digital holography

    NASA Astrophysics Data System (ADS)

    Hernandez-Montes, Maria del Socorro; Mendoza Santoyo, Fernando; Perez-Lopez, Carlos

    2004-08-01

    An out of plane optical sensitive configuration for pulsed digital holography was used to detect inhomogeneities inside semi solid organic materials. A loud speaker was employed to produce a mechanical wave that propagates through the material in such a way that it generates vibrational resonant modes and transient events on the material surface. Surface micro displacements were observed between the firing of two consecutive laser pulses, both for a steady resonant mode and for different times during the transient event. Two kinds of inhomogeneities were inserted approximately 2 cm inside the material diffracting the original mechanical wave and thus changing the resonant mode pattern or the transient wave on the surface. Comparison of phase unwrapped patterns, with and without inhomogeneities allows the rapid identification of their existence. The results for the resonant and transient conditions show that the method may be reliably used to study, compare and distinguish data from inside homogeneous and in-homogeneous organic materials.

  7. Absolute measurement of aspheric lens with electrically tunable lens in digital holography

    NASA Astrophysics Data System (ADS)

    Wang, Zhaomin; Qu, Weijuan; Yang, Fang; Tian, Ailing; Asundi, Anand

    2017-01-01

    A novel method for testing aspheric lenses using digital holography with an electrically tunable lens (ETL) is proposed and experimentally verified. The ETL generates a tunable deformed wavefront which helps to decrease the high gradient of aspheric lenses. By decomposing the aspheric surface into two resolvable ones, its absolute phase can be determined using a double-exposure measurement. In this method, the wavefront generated by the ETL need not be identical to the aspheric surface as in conventional null interferometer system, but just sufficient to resolve the high gradient surface. On the other hand, the tunability of the ETL allows generation of wavefronts which can be used to test different aspheric lens. Furthermore, advantages of the ETL such as low cost, fast response, and compact configuration make the proposed method a promising technique for aspheric surface measurement.

  8. Self-patterning of a polydimethylsiloxane microlens array on functionalized substrates and characterization by digital holography

    NASA Astrophysics Data System (ADS)

    Merola, F.; Paturzo, M.; Coppola, S.; Vespini, V.; Ferraro, P.

    2009-12-01

    Microlens arrays are realized through a self-arrangement process of thin liquid polymeric polydimethylsiloxane (PDMS) film on a functionalized polar dielectric crystal substrate. The self-arrangement process is named the pyro-electro-wetting mechanism. The substrate, a LiNbO3 (LN) z-cut wafer, has been micro-engineered with periodically poled ferroelectric domains, with the aim to provide an appropriate wettability patterning induced by a thermal stimulus. Different experimental procedures have been explored demonstrating that arrays of thousands of microlenses, having a diameter size of 100 µm and focal lengths ranging between 300 and 1100 µm, can be fabricated. Furthermore, a microscope interference method based on digital holography is adopted for microlens characterization.

  9. Multilevel bidimensional empirical mode decomposition: a new speckle reduction method in digital holography

    NASA Astrophysics Data System (ADS)

    Leo, Marco; Piccolo, Roberta; Distante, Cosimo; Memmolo, Pasquale; Paturzo, Melania; Ferraro, Pietro

    2014-11-01

    The paper presents a new automatic technique for speckle reduction in the context of digital holography. Speckle noise is a superposition of unwanted spots over objects of interest, due to the behavior of a coherence source of radiation with the object surface characteristics. In the proposed denoising method, bidimensional empirical mode decomposition is used to decompose the image signal, which is then filtered through the Frost filter. The proposed technique was preliminarily tested on the "Lena" image for quality assessment in terms of peak signal-to-noise ratio. Then, its denoising capability was assessed on different holographic images on which also the comparison (using both blind metrics and visual inspection) with the leading strategies in the state of the art was favorably performed.

  10. Sparsity-Based Pixel Super Resolution for Lens-Free Digital In-line Holography

    PubMed Central

    Song, Jun; Leon Swisher, Christine; Im, Hyungsoon; Jeong, Sangmoo; Pathania, Divya; Iwamoto, Yoshiko; Pivovarov, Misha; Weissleder, Ralph; Lee, Hakho

    2016-01-01

    Lens-free digital in-line holography (LDIH) is a promising technology for portable, wide field-of-view imaging. Its resolution, however, is limited by the inherent pixel size of an imaging device. Here we present a new computational approach to achieve sub-pixel resolution for LDIH. The developed method is a sparsity-based reconstruction with the capability to handle the non-linear nature of LDIH. We systematically characterized the algorithm through simulation and LDIH imaging studies. The method achieved the spatial resolution down to one-third of the pixel size, while requiring only single-frame imaging without any hardware modifications. This new approach can be used as a general framework to enhance the resolution in nonlinear holographic systems. PMID:27098438

  11. Depth quantificaton for inhomogeneities within semisolid materials using 3D pulsed digital holography

    NASA Astrophysics Data System (ADS)

    Hernández-Montes, Maria del Socorro; Mendoza Santoyo, Fernando; Pérez-López, Carlos; Rodríguez Vera, Ramón

    2006-06-01

    A 3D experimental arrangement for pulsed digital holography is used to measure the depth position for both a glass sphere and tumor tissue, immersed in a semi-solid gel model. A master gel, one without inhomogeneities, is set to resonate via sound waves generated with a conventional speaker placed a few centimeters away from the gel container. Later an identical prepared gel with an inhomogeneity is placed in the original set up and interrogated at the same resonant frequency. On comparison and using only an out of plane sensitive set up it is possible to measure the displacement of the gel surface, indicating the presence of an internal inhomogeneity. However the depth position cannot be measured accurately since the out of plane component has also the contribution of the in-plane surface displacements. With the information gathered from the 3D pulsed digital holography set up it is possible to obtain three sensitivity vectors that serve to independently separate the contributions from each of the three x, y and z components of the vibration displacement, for the same exciting mechanical wave. It is then possible to build individual maps of displacement along the three rectangular axes and thus measure accurately the depth position of the inhomogeneity. Results from the optical data were correlated to the measured position for different inhomogeneity types, sizes and depths and on comparison an error in the position of less than 1% was found. This optical non invasive method is able to accurately find the inhomogeneity and its position within the gel making it a promising method for the study of mammal tumors, representing and alternative to the traditional invasive methods.

  12. Acquisition and replay systems for direct-to-digital holography and holovision

    DOEpatents

    Thomas, Clarence E.; Hanson, Gregory R.

    2003-02-25

    Improvements to the acquisition and replay systems for direct-to-digital holography and holovision are described. A method of recording an off-axis hologram includes: splitting a laser beam into an object beam and a reference beam; reflecting the reference beam from a reference beam mirror; reflecting the object beam from an illumination beamsplitter; passing the object beam through an objective lens; reflecting the object beam from an object; focusing the reference beam and the object beam at a focal plane of a digital recorder to form an off-axis hologram; digitally recording the off-axis hologram; and transforming the off-axis hologram in accordance with a Fourier transform to obtain a set of results. A method of writing an off-axis hologram includes: passing a laser beam through a spatial light modulator; and focusing the laser beam at a focal plane of a photorefractive crystal to impose a holographic diffraction grating pattern on the photorefractive crystal. A method of replaying an off-axis hologram includes: illuminating a photorefractive crystal having a holographic diffraction grating with a replay beam.

  13. Comparative analysis of autofocus functions in digital in-line phase-shifting holography.

    PubMed

    Fonseca, Elsa S R; Fiadeiro, Paulo T; Pereira, Manuela; Pinheiro, António

    2016-09-20

    Numerical reconstruction of digital holograms relies on a precise knowledge of the original object position. However, there are a number of relevant applications where this parameter is not known in advance and an efficient autofocusing method is required. This paper addresses the problem of finding optimal focusing methods for use in reconstruction of digital holograms of macroscopic amplitude and phase objects, using digital in-line phase-shifting holography in transmission mode. Fifteen autofocus measures, including spatial-, spectral-, and sparsity-based methods, were evaluated for both synthetic and experimental holograms. The Fresnel transform and the angular spectrum reconstruction methods were compared. Evaluation criteria included unimodality, accuracy, resolution, and computational cost. Autofocusing under angular spectrum propagation tends to perform better with respect to accuracy and unimodality criteria. Phase objects are, generally, more difficult to focus than amplitude objects. The normalized variance, the standard correlation, and the Tenenbaum gradient are the most reliable spatial-based metrics, combining computational efficiency with good accuracy and resolution. A good trade-off between focus performance and computational cost was found for the Fresnelet sparsity method.

  14. Fused off-axis object illumination direct-to-digital holography with a plurality of illumination sources

    DOEpatents

    Price, Jeffery R.; Bingham, Philip R.

    2005-11-08

    Systems and methods are described for rapid acquisition of fused off-axis illumination direct-to-digital holography. A method of recording a plurality of off-axis object illuminated spatially heterodyne holograms, each of the off-axis object illuminated spatially heterodyne holograms including spatially heterodyne fringes for Fourier analysis, includes digitally recording, with a first illumination source of an interferometer, a first off-axis object illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis; and digitally recording, with a second illumination source of the interferometer, a second off-axis object illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis.

  15. The influence of hologram aperture on speckle noise in the reconstructed image of digital holography and its reduction

    NASA Astrophysics Data System (ADS)

    Cai, Xiao-ou; Wang, Hui

    2008-01-01

    Based on the whole process of the recording and reconstruction of digital holography, we study the formation cause of speckle noise in its reconstructed image and acquire the conclusion that the small size of hologram aperture diffraction aggravates the speckle noise of reconstructed image and the speckle noise has been one of primary noise sources in the reconstruction process. In order to reduce the speckle noise resulting from little hologram aperture diffraction, we set an appropriate aperture function matching the recording parameter and aperture size of hologram and deconvolve the reconstructed image with it. The validity has been proved in theory and experiment. Therefore, it offers a brand-new thought and practical way to reduce the speckle noise in the reconstructed image of digital holography.

  16. Three-dimensional imaging of distribution of refractive index by parallel phase-shifting digital holography using Abel inversion.

    PubMed

    Fukuda, Takahito; Wang, Yexin; Xia, Peng; Awatsuji, Yasuhiro; Kakue, Takashi; Nishio, Kenzo; Matoba, Osamu

    2017-07-24

    Although digital holography is a powerful technique obtaining a phase image of a transparent object, the image reconstructed by the technique merely expresses phase distribution of the light wave after transmitting through the object. Phase variation of inside of the object is difficult to be obtained. Then, we applied Abel inversion method to the high-speed phase image of a dynamic transparent object assumed axially symmetric. The phase is accurately recorded by phase-shifting method. We experimentally recorded transparent dynamic gas flow, assumed axially symmetric along the direction in which gas flowed, at 3,000 frame/s and reconstructed motion picture of 3D distribution of the refractive index of the gas from the high-speed phase motion picture obtained by parallel phase-shifting digital holography.

  17. Simultaneous amplitude-contrast and phase-contrast surface plasmon resonance imaging by use of digital holography

    PubMed Central

    Li, Shiping; Zhong, Jingang

    2012-01-01

    The surface plasmon resonance imaging technique provides a tool that allows high-throughput analysis and real-time kinetic measurement. A simultaneous amplitude-contrast and phase-contrast surface plasmon resonance imaging method is presented. The amplitude-contrast and phase-contrast images are simultaneously obtained by use of digital holography. The detection sensitivity of amplitude-contrast imaging and phase-contrast imaging can compensate for each other. Thus, the detectable sample components may cover a wider range of refractive index values for the simultaneous amplitude-contrast and phase-contrast imaging method than for the phase-contrast imaging method or amplitude-contrast imaging method. A detailed description of the theory and an experiment of monitoring the evaporation process of a drop of NaCl injection in real time are presented. In addition, the amplitude-contrast image has less coherent noise by digital holography. PMID:23243569

  18. Evaluating unsupervised methods to size and classify suspended particles using digital in-line holography

    USGS Publications Warehouse

    Davies, Emlyn J.; Buscombe, Daniel D.; Graham, George W.; Nimmo-Smith, W. Alex M.

    2015-01-01

    Substantial information can be gained from digital in-line holography of marine particles, eliminating depth-of-field and focusing errors associated with standard lens-based imaging methods. However, for the technique to reach its full potential in oceanographic research, fully unsupervised (automated) methods are required for focusing, segmentation, sizing and classification of particles. These computational challenges are the subject of this paper, in which we draw upon data collected using a variety of holographic systems developed at Plymouth University, UK, from a significant range of particle types, sizes and shapes. A new method for noise reduction in reconstructed planes is found to be successful in aiding particle segmentation and sizing. The performance of an automated routine for deriving particle characteristics (and subsequent size distributions) is evaluated against equivalent size metrics obtained by a trained operative measuring grain axes on screen. The unsupervised method is found to be reliable, despite some errors resulting from over-segmentation of particles. A simple unsupervised particle classification system is developed, and is capable of successfully differentiating sand grains, bubbles and diatoms from within the surf-zone. Avoiding miscounting bubbles and biological particles as sand grains enables more accurate estimates of sand concentrations, and is especially important in deployments of particle monitoring instrumentation in aerated water. Perhaps the greatest potential for further development in the computational aspects of particle holography is in the area of unsupervised particle classification. The simple method proposed here provides a foundation upon which further development could lead to reliable identification of more complex particle populations, such as those containing phytoplankton, zooplankton, flocculated cohesive sediments and oil droplets.

  19. Single-exposure phase-shifting digital holography using a random-complex-amplitude encoded reference wave.

    PubMed

    Imbe, Masatoshi; Nomura, Takanori

    2013-01-01

    The improved single-exposure phase-shifting digital holography using a random-phase reference wave is proposed. The algorithm for obtaining a complex amplitude of an object wave is improved. In the proposed algorithm, the reference wave is treated as not a random-phase but a random-complex amplitude. Therefore, the algorithm uses proper amplitude information of the reference wave. Both numerical simulations and experimental results are given to confirm the effectiveness of the proposed algorithm.

  20. Selective calculation for the improvement of reconstructed images in single-exposure generalized phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Imbe, Masatoshi; Nomura, Takanori

    2014-04-01

    Single-exposure generalized phase-shifting digital holography enables one to remove the zeroth-order and conjugate terms from the single hologram. For the phase-shifting, a random-complex-amplitude encoded wave is used as the reference wave. This encoded wave can be made by a commercially available element. Selective calculation is proposed for the improvement of reconstructed images in single-exposure generalized phase-shifting digital holography. Three calculations are used as a function of the complex amplitude quantities of the reference wave on the each pixel of the image sensor. The selective calculation improves the quality of the reconstructed image compared with applying only one of the three calculations to the hologram. The derivation of the three calculations and the reason why the number of calculations is three are presented. A parameter is defined and used to select the appropriate calculation from the three calculations. Relationships between the proposed method and the previous method for the improvement of the reconstructed images in single-exposure generalized phase-shifting digital holography are discussed. Two kinds of experimental results are given to confirm the effectiveness of the proposed method. One shows the dependence on the speckle size of the reference wave. The other shows the feasibility to dynamic phenomena.

  1. Denoising algorithm based on edge extraction and wavelet transform in digital holography

    NASA Astrophysics Data System (ADS)

    Zhang, Ming; Sang, Xin-zhu; Leng, Jun-min; Cao, Xue-mei

    2013-08-01

    Digital holography is a kind of coherent imaging method and inevitably affected by many factors in the process of recording. One of dominant problems is the speckle noise, which is essentially nonlinear multiplicative noise related to signals. So it is more difficult to remove than additive noise. Due to the noise pollution, the low resolution of image reconstructed is caused. A new solution for suppressing speckle noise in digital hologram is presented, which combines Canny filtering algorithm with wavelet threshold denoising algorithm. Canny filter is used to obtain the edge detail. Wavelet transformation performs denoising. In order to suppress speckle effectively and retain the image details as much as possible, Neyman-Pearson (N-P) criterion is introduced to estimate wavelet coefficient in every scale. An improved threshold function is proposed, whose curve is smoother. The reconstructed image is achieved by merging the denoised image with the edge details. Experimental results and performance parameters of the proposed algorithm are discussed and compared with other methods, which shows that the presented approach can not only effectively eliminate speckle noise, but also retain useful signals and edge information simultaneously.

  2. Numerical investigation of the effect of particle concentration on particle measurement by digital holography

    NASA Astrophysics Data System (ADS)

    Zhao, Huafeng; Zhou, Binwu; Wu, Xuecheng; Wu, Yingchun; Gao, Xiang; Gréhan, Gérard; Cen, Kefa

    2014-04-01

    Digital holography plays a key role in particle field measurement, and appears to be a strong contender as the next-generation technology for diagnostics of 3D particle field. However, various recording parameters, such as the recording distance, the particle size, the wavelength, the size of the CCD chip, the pixel size and the particle concentration, will affect the results of the reconstruction, and may even determine the success or failure of a measurement. This paper presents a numerical investigation on the effect of particle concentration, the volume depth to evaluate the capability of digital holographic microscopy. Standard particles holograms with all known recording parameters are numerically generated by using a common procedure based on Lorenz-Mie scattering theory. Reconstruction of those holograms are then performed by a wavelet-transform based method. Results show that the reconstruction efficiency decreases quickly until particle concentration reaches 50×104 (mm-3), and decreases linearly with the increase of particle concentration from 50 × 104 (mm-3) to 860 × 104 (mm-3) in the same volume. The first half of the line waves larger than the second half. It also indicates that the increase of concentration leads the rise in average diameter error and z position error of particles. Besides, the volume depth also plays a key role in reconstruction.

  3. Testing silicon MEMS structures subjected to thermal loading by digital holography

    NASA Astrophysics Data System (ADS)

    Ferraro, Pietro; De Nicola, Sergio; Coppola, Giuseppe; Finizio, Andrea; Iodice, Mario; Magro, Carlo; Pierattini, Giovanni

    2004-01-01

    We have applied digital holography (DH) as interferometric tool for measuring the out of plane deformation of Micro-Electro-Mechanical structures. DH has been adopted as method for determining with high accuracy deformations due to the residual stress introduced by fabrication process evaluating MEMS behavior subjected to thermal load. A thermal characterization of these structures requires to cope two fundamental problems. The first one regards the loss of the focus due to thermal expansion of the MEMS sample support. With an out-of-focus image, a correct reconstruction of the sample image can not be obtained. To overcome the problem an auto-tracking procedure has been adopted. The other problem regards the direct comparison of images reconstructed at two different distances. In fact, in DH the numerical reconstruction image is enlarged or contracted according to the reconstruction distance. To avoid this problem, we have adopted a novel but very simple method for keeping constant the image size by imposing the reconstruction pixel constant through the fictitious enlargement of the number of the pixel of the recorded digital holograms. These procedures have been employed in order to characterize MEMS with different shapes and dimensions. The measured profiles obtained by DH can be employed to evaluate both the residual stress induced during the fabrication processes and its dependence on the temperature.

  4. Testing silicon MEMS structures subjected to thermal loading by digital holography

    NASA Astrophysics Data System (ADS)

    Ferraro, Pietro; De Nicola, Sergio; Coppola, Giuseppe; Finizio, Andrea; Iodice, Mario; Magro, Carlo; Pierattini, Giovanni

    2003-12-01

    We have applied digital holography (DH) as interferometric tool for measuring the out of plane deformation of Micro-Electro-Mechanical structures. DH has been adopted as method for determining with high accuracy deformations due to the residual stress introduced by fabrication process evaluating MEMS behavior subjected to thermal load. A thermal characterization of these structures requires to cope two fundamental problems. The first one regards the loss of the focus due to thermal expansion of the MEMS sample support. With an out-of-focus image, a correct reconstruction of the sample image can not be obtained. To overcome the problem an auto-tracking procedure has been adopted. The other problem regards the direct comparison of images reconstructed at two different distances. In fact, in DH the numerical reconstruction image is enlarged or contracted according to the reconstruction distance. To avoid this problem, we have adopted a novel but very simple method for keeping constant the image size by imposing the reconstruction pixel constant through the fictitious enlargement of the number of the pixel of the recorded digital holograms. These procedures have been employed in order to characterize MEMS with different shapes and dimensions. The measured profiles obtained by DH can be employed to evaluate both the residual stress induced during the fabrication processes and its dependence on the temperature.

  5. A novel image watermarking method based on singular value decomposition and digital holography

    NASA Astrophysics Data System (ADS)

    Cai, Zhishan

    2016-10-01

    According to the information optics theory, a novel watermarking method based on Fourier-transformed digital holography and singular value decomposition (SVD) is proposed in this paper. First of all, a watermark image is converted to a digital hologram using the Fourier transform. After that, the original image is divided into many non-overlapping blocks. All the blocks and the hologram are decomposed using SVD. The singular value components of the hologram are then embedded into the singular value components of each block using an addition principle. Finally, SVD inverse transformation is carried out on the blocks and hologram to generate the watermarked image. The watermark information embedded in each block is extracted at first when the watermark is extracted. After that, an averaging operation is carried out on the extracted information to generate the final watermark information. Finally, the algorithm is simulated. Furthermore, to test the encrypted image's resistance performance against attacks, various attack tests are carried out. The results show that the proposed algorithm has very good robustness against noise interference, image cut, compression, brightness stretching, etc. In particular, when the image is rotated by a large angle, the watermark information can still be extracted correctly.

  6. The applicability of holography in forensic identification: a fusion of the traditional optical technique and digital technique.

    PubMed

    Biwasaka, Hitoshi; Saigusa, Kiyoshi; Aoki, Yasuhiro

    2005-03-01

    In this study, the applicability of holography in the 3-dimensional recording of forensic objects such as skulls and mandibulae, and the accuracy of the reconstructed 3-D images, were examined. The virtual holographic image, which records the 3-dimensional data of the original object, is visually observed on the other side of the holographic plate, and reproduces the 3-dimensional shape of the object well. Another type of holographic image, the real image, is focused on a frosted glass screen, and cross-sectional images of the object can be observed. When measuring the distances between anatomical reference points using an image-processing software, the average deviations in the holographic images as compared to the actual objects were less than 0.1 mm. Therefore, holography could be useful as a 3-dimensional recording method of forensic objects. Two superimposition systems using holographic images were examined. In the 2D-3D system, the transparent virtual holographic image of an object is directly superimposed onto the digitized photograph of the same object on the LCD monitor. On the other hand, in the video system, the holographic image captured by the CCD camera is superimposed onto the digitized photographic image using a personal computer. We found that the discrepancy between the outlines of the superimposed holographic and photographic dental images using the video system was smaller than that using the 2D-3D system. Holography seemed to perform comparably to the computer graphic system; however, a fusion with the digital technique would expand the utility of holography in superimposition.

  7. High resolution digital holographic microscopy with a wide field of view based on a synthetic aperture technique and use of linear CCD scanning.

    PubMed

    Di, Jianglei; Zhao, Jianlin; Jiang, Hongzhen; Zhang, Peng; Fan, Qi; Sun, Weiwei

    2008-10-20

    Theoretical analysis shows that, to improve the resolution and the range of the field of view of the reconstructed image in digital lensless Fourier transform holography, an effective solution is to increase the area and the pixel number of the recorded digital hologram. A new approach based on the synthetic aperture technique and use of linear CCD scanning is presented to obtain digital holographic images with high resolution and a wide field of view. By using a synthetic aperture technique and linear CCD scanning, we obtained digital lensless Fourier transform holograms with a large area of 3.5 cm x 3.5 cm (5000 x 5000 pixels). The numerical reconstruction of a 4 mm object at a distance of 14 cm by use of a Rayleigh-Sommerfeld integral shows that a theoretically minimum resolvable distance of 2.57 microm can be achieved at a wavelength of 632.8 nm. The experimental results are consistent with the theoretical analysis.

  8. Phase-shifting digital holography with a low-coherence light source for reconstruction of a digital relief object hidden behind a light-scattering medium.

    PubMed

    Tamano, Shingo; Hayasaki, Yoshio; Nishida, Nobuo

    2006-02-10

    Hiding image data with a light-scattering medium is effective as a basic data protection technique. The hidden image data can be observed only by using a low-coherence interference technique and is thus protected from unauthorized access. Unlike an intensity-distributed object, a digital relief object has no intensity distribution, making it possible to hide its existence by using a dilute light-scattering medium. To reconstruct the digital relief object through the light-scattering medium, we developed phase-shifting digital holography with a low-coherence light source. The experimental performance, including the spatial resolution and phase error of the reconstructed image, is estimated.

  9. Computer-generated real-time digital holography: first time use in clinical medical imaging.

    PubMed

    Bruckheimer, Elchanan; Rotschild, Carmel; Dagan, Tamir; Amir, Gabriel; Kaufman, Aviad; Gelman, Shaul; Birk, Einat

    2016-08-01

    Assessment of the feasibility of creating real-time interactive 3D digital holograms in a standard catheterization laboratory. 3D medical images are typically displayed and interacted with on 2D screens limiting their usefulness. A digital computer-generated real-time holographic display of patient's 3D data could provide a spatially accurate image with all the depth cues and afford interaction within the image. We performed a feasibility study of creating real-time interactive 3D digital holograms with a purpose-built prototype using intraprocedural data from 3D rotational angiography and live 3D transesophageal echocardiography. The primary objective was to demonstrate that all the anatomical landmarks identified on standard imaging can be similarly identified using dynamic and static holographic images. The secondary objective was to demonstrate the usability of interactions with the image. Parameters were assessed by a rating scale. Eight patients were enrolled of whom five underwent transcatheter ASD closure using 3DTEE and three patients were evaluated by 3D rotational angiography. In all cases dynamic real-time and static 3D holograms were created in standard cath lab conditions. Four individual observers identified all anatomical landmarks on the holographic display independently from the 2D display. Interactions with the hologram including marking, cropping and rotation were performed. There were no adverse events. This study demonstrates, for the first time, the feasibility of generating high quality, clinically relevant, 3D real-time colour dynamic holograms in a standard clinical setting with real patient volumetric data. The impact of computer-generated holography needs to be evaluated in controlled clinical trials. Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2016. For permissions please email: journals.permissions@oup.com.

  10. Comparison of three-dimensional particle tracking and sizing using plenoptic imaging and digital in-line holography

    DOE PAGES

    Hall, Elise M.; Thurow, Brian S.; Guildenbecher, Daniel R.

    2016-08-08

    Digital in-line holography (DIH) and plenoptic photography are two techniques for single-shot, volumetric measurement of 3D particle fields. Here we present a comparison of the two methods by applying plenoptic imaging to experimental configurations that have been previously investigated with DIH. These experiments include the tracking of secondary droplets from the impact of a water drop on a thin film of water and tracking of pellets from a shotgun. Both plenoptic imaging and DIH successfully quantify the 3D nature of these particle fields. Furthermore, this includes measurement of the 3D particle position, individual particle sizes, and three-component velocity vectors. Formore » the initial processing methods presented here, both techniques give out-of-plane positional accuracy of approximately 1–2 particle diameters. For a fixed image sensor, digital holography achieves higher effective in-plane spatial resolutions. However, collimated and coherent illumination makes holography susceptible to image distortion through index of refraction gradients, as demonstrated in the shotgun experiments. In contrast, plenoptic imaging allows for a simpler experimental configuration and, due to the use of diffuse, white-light illumination, plenoptic imaging is less susceptible to image distortion in the shotgun experiments.« less

  11. Comparison of three-dimensional particle tracking and sizing using plenoptic imaging and digital in-line holography

    SciTech Connect

    Hall, Elise M.; Thurow, Brian S.; Guildenbecher, Daniel R.

    2016-08-08

    Digital in-line holography (DIH) and plenoptic photography are two techniques for single-shot, volumetric measurement of 3D particle fields. Here we present a comparison of the two methods by applying plenoptic imaging to experimental configurations that have been previously investigated with DIH. These experiments include the tracking of secondary droplets from the impact of a water drop on a thin film of water and tracking of pellets from a shotgun. Both plenoptic imaging and DIH successfully quantify the 3D nature of these particle fields. Furthermore, this includes measurement of the 3D particle position, individual particle sizes, and three-component velocity vectors. For the initial processing methods presented here, both techniques give out-of-plane positional accuracy of approximately 1–2 particle diameters. For a fixed image sensor, digital holography achieves higher effective in-plane spatial resolutions. However, collimated and coherent illumination makes holography susceptible to image distortion through index of refraction gradients, as demonstrated in the shotgun experiments. In contrast, plenoptic imaging allows for a simpler experimental configuration and, due to the use of diffuse, white-light illumination, plenoptic imaging is less susceptible to image distortion in the shotgun experiments.

  12. Comparison of three-dimensional particle tracking and sizing using plenoptic imaging and digital in-line holography.

    PubMed

    Hall, Elise M; Thurow, Brian S; Guildenbecher, Daniel R

    2016-08-10

    Digital in-line holography (DIH) and plenoptic photography are two techniques for single-shot, volumetric measurement of 3D particle fields. Here we present a comparison of the two methods by applying plenoptic imaging to experimental configurations that have been previously investigated with DIH. These experiments include the tracking of secondary droplets from the impact of a water drop on a thin film of water and tracking of pellets from a shotgun. Both plenoptic imaging and DIH successfully quantify the 3D nature of these particle fields. This includes measurement of the 3D particle position, individual particle sizes, and three-component velocity vectors. For the initial processing methods presented here, both techniques give out-of-plane positional accuracy of approximately 1-2 particle diameters. For a fixed image sensor, digital holography achieves higher effective in-plane spatial resolutions. However, collimated and coherent illumination makes holography susceptible to image distortion through index of refraction gradients, as demonstrated in the shotgun experiments. In contrast, plenoptic imaging allows for a simpler experimental configuration and, due to the use of diffuse, white-light illumination, plenoptic imaging is less susceptible to image distortion in the shotgun experiments.

  13. Comparison of three-dimensional particle tracking and sizing using plenoptic imaging and digital in-line holography

    SciTech Connect

    Hall, Elise M.; Thurow, Brian S.; Guildenbecher, Daniel R.

    2016-08-08

    Digital in-line holography (DIH) and plenoptic photography are two techniques for single-shot, volumetric measurement of 3D particle fields. Here we present a comparison of the two methods by applying plenoptic imaging to experimental configurations that have been previously investigated with DIH. These experiments include the tracking of secondary droplets from the impact of a water drop on a thin film of water and tracking of pellets from a shotgun. Both plenoptic imaging and DIH successfully quantify the 3D nature of these particle fields. Furthermore, this includes measurement of the 3D particle position, individual particle sizes, and three-component velocity vectors. For the initial processing methods presented here, both techniques give out-of-plane positional accuracy of approximately 1–2 particle diameters. For a fixed image sensor, digital holography achieves higher effective in-plane spatial resolutions. However, collimated and coherent illumination makes holography susceptible to image distortion through index of refraction gradients, as demonstrated in the shotgun experiments. In contrast, plenoptic imaging allows for a simpler experimental configuration and, due to the use of diffuse, white-light illumination, plenoptic imaging is less susceptible to image distortion in the shotgun experiments.

  14. Novel Perspectives on the Characterization of Species-Dependent Optical Signatures of Bacterial Colonies by Digital Holography.

    PubMed

    Buzalewicz, Igor; Kujawińska, Małgorzata; Krauze, Wojciech; Podbielska, Halina

    2016-01-01

    The use of light diffraction for the microbiological diagnosis of bacterial colonies was a significant breakthrough with widespread implications for the food industry and clinical practice. We previously confirmed that optical sensors for bacterial colony light diffraction can be used for bacterial identification. This paper is focused on the novel perspectives of this method based on digital in-line holography (DIH), which is able to reconstruct the amplitude and phase properties of examined objects, as well as the amplitude and phase patterns of the optical field scattered/diffracted by the bacterial colony in any chosen observation plane behind the object from single digital hologram. Analysis of the amplitude and phase patterns inside a colony revealed its unique optical properties, which are associated with the internal structure and geometry of the bacterial colony. Moreover, on a computational level, it is possible to select the desired scattered/diffracted pattern within the entire observation volume that exhibits the largest amount of unique, differentiating bacterial features. These properties distinguish this method from the already proposed sensing techniques based on light diffraction/scattering of bacterial colonies. The reconstructed diffraction patterns have a similar spatial distribution as the recorded Fresnel patterns, previously applied for bacterial identification with over 98% accuracy, but they are characterized by both intensity and phase distributions. Our results using digital holography provide new optical discriminators of bacterial species revealed in one single step in form of new optical signatures of bacterial colonies: digital holograms, reconstructed amplitude and phase patterns, as well as diffraction patterns from all observation space, which exhibit species-dependent features. To the best of our knowledge, this is the first report on bacterial colony analysis via digital holography and our study represents an innovative approach

  15. Novel Perspectives on the Characterization of Species-Dependent Optical Signatures of Bacterial Colonies by Digital Holography

    PubMed Central

    Buzalewicz, Igor; Kujawińska, Małgorzata; Krauze, Wojciech; Podbielska, Halina

    2016-01-01

    The use of light diffraction for the microbiological diagnosis of bacterial colonies was a significant breakthrough with widespread implications for the food industry and clinical practice. We previously confirmed that optical sensors for bacterial colony light diffraction can be used for bacterial identification. This paper is focused on the novel perspectives of this method based on digital in-line holography (DIH), which is able to reconstruct the amplitude and phase properties of examined objects, as well as the amplitude and phase patterns of the optical field scattered/diffracted by the bacterial colony in any chosen observation plane behind the object from single digital hologram. Analysis of the amplitude and phase patterns inside a colony revealed its unique optical properties, which are associated with the internal structure and geometry of the bacterial colony. Moreover, on a computational level, it is possible to select the desired scattered/diffracted pattern within the entire observation volume that exhibits the largest amount of unique, differentiating bacterial features. These properties distinguish this method from the already proposed sensing techniques based on light diffraction/scattering of bacterial colonies. The reconstructed diffraction patterns have a similar spatial distribution as the recorded Fresnel patterns, previously applied for bacterial identification with over 98% accuracy, but they are characterized by both intensity and phase distributions. Our results using digital holography provide new optical discriminators of bacterial species revealed in one single step in form of new optical signatures of bacterial colonies: digital holograms, reconstructed amplitude and phase patterns, as well as diffraction patterns from all observation space, which exhibit species-dependent features. To the best of our knowledge, this is the first report on bacterial colony analysis via digital holography and our study represents an innovative approach

  16. Real-time terahertz digital holography with a quantum cascade laser

    PubMed Central

    Locatelli, Massimiliano; Ravaro, Marco; Bartalini, Saverio; Consolino, Luigi; Vitiello, Miriam S.; Cicchi, Riccardo; Pavone, Francesco; De Natale, Paolo

    2015-01-01

    Coherent imaging in the THz range promises to exploit the peculiar capabilities of these wavelengths to penetrate common materials like plastics, ceramics, paper or clothes with potential breakthroughs in non-destructive inspection and quality control, homeland security and biomedical applications. Up to now, however, THz coherent imaging has been limited by time-consuming raster scanning, point-like detection schemes and by the lack of adequate coherent sources. Here, we demonstrate real-time digital holography (DH) at THz frequencies exploiting the high spectral purity and the mW output power of a quantum cascade laser combined with the high sensitivity and resolution of a microbolometric array. We show that, in a one-shot exposure, phase and amplitude information of whole samples, either in reflection or in transmission, can be recorded. Furthermore, a 200 times reduced sensitivity to mechanical vibrations and a significantly enlarged field of view are observed, as compared to DH in the visible range. These properties of THz DH enable unprecedented holographic recording of real world dynamic scenes. PMID:26315647

  17. Calculation reduction method for color digital holography and computer-generated hologram using color space conversion

    NASA Astrophysics Data System (ADS)

    Shimobaba, Tomoyoshi; Nagahama, Yuki; Kakue, Takashi; Takada, Naoki; Okada, Naohisa; Endo, Yutaka; Hirayama, Ryuji; Hiyama, Daisuke; Ito, Tomoyoshi

    2014-02-01

    A calculation reduction method for color digital holography (DH) and computer-generated holograms (CGHs) using color space conversion is reported. Color DH and color CGHs are generally calculated on RGB space. We calculate color DH and CGHs in other color spaces for accelerating the calculation (e.g., YCbCr color space). In YCbCr color space, a RGB image or RGB hologram is converted to the luminance component (Y), blue-difference chroma (Cb), and red-difference chroma (Cr) components. In terms of the human eye, although the negligible difference of the luminance component is well recognized, the difference of the other components is not. In this method, the luminance component is normal sampled and the chroma components are down-sampled. The down-sampling allows us to accelerate the calculation of the color DH and CGHs. We compute diffraction calculations from the components, and then we convert the diffracted results in YCbCr color space to RGB color space. The proposed method, which is possible to accelerate the calculations up to a factor of 3 in theory, accelerates the calculation over two times faster than the ones in RGB color space.

  18. Terahertz in-line digital holography of human hepatocellular carcinoma tissue

    PubMed Central

    Rong, Lu; Latychevskaia, Tatiana; Chen, Chunhai; Wang, Dayong; Yu, Zhengping; Zhou, Xun; Li, Zeyu; Huang, Haochong; Wang, Yunxin; Zhou, Zhou

    2015-01-01

    Terahertz waves provide a better contrast in imaging soft biomedical tissues than X-rays, and unlike X-rays, they cause no ionisation damage, making them a good option for biomedical imaging. Terahertz absorption imaging has conventionally been used for cancer diagnosis. However, the absorption properties of a cancerous sample are influenced by two opposing factors: an increase in absorption due to a higher degree of hydration and a decrease in absorption due to structural changes. It is therefore difficult to diagnose cancer from an absorption image. Phase imaging can thus be critical for diagnostics. We demonstrate imaging of the absorption and phase-shift distributions of 3.2 mm × 2.3 mm × 30-μm-thick human hepatocellular carcinoma tissue by continuous-wave terahertz digital in-line holography. The acquisition time of a few seconds for a single in-line hologram is much shorter than that of other terahertz diagnostic techniques, and future detectors will allow acquisition of meaningful holograms without sample dehydration. The resolution of the reconstructions was enhanced by sub-pixel shifting and extrapolation. Another advantage of this technique is its relaxed minimal sample size limitation. The fibrosis indicated in the phase distribution demonstrates the potential of terahertz holographic imaging to obtain a more objective, early diagnosis of cancer. PMID:25676705

  19. Direct To Digital Holography For High Aspect Ratio Inspection of Semiconductor Wafers

    NASA Astrophysics Data System (ADS)

    Thomas, C. E. (Tommy); Hunt, Martin A.; Bahm, Tracy M.; Baylor, Larry R.; Bingham, Philip R.; Chidley, Matthew D.; Dai, Xiaolong; Delahanty, Robert J.; El-Khashab, Ayman; Gilbert, Judd M.; Goddard, James S.; Hanson, Gregory R.; Hickson, Joel D.; Hylton, Kathy W.; John, George C.; Jones, Michael L.; Mayo, Michael W.; Marek, Christopher; Price, John H.; Rasmussen, David A.; Schaefer, Louis J.; Schulze, Mark A.; Shen, Bichuan; Smith, Randall G.; Su, Allen N.; Tobin, Kenneth W.; Usry, William R.; Voelkl, Edgar; Weber, Karsten S.; Owen, Robert W.

    2003-09-01

    Direct to Digital Holography (DDH) has been developed as a semiconductor wafer inspection tool and in particular as a tool for seeing defects in high aspect ratio (HAR) structures on semiconductor wafers and also for seeing partial-height defects. While the tool works very well for general wafer inspection, it has unusual capabilities for high aspect ratio inspection (HARI) and for detecting thin residual film defects (partial height defects). Inspection of HAR structures is rated as one of the highest unmet priorities of the member companies of International SEMATECH, and finding residual thin film defects (in some cases called "stringers") is also a very difficult challenge. The capabilities that make DDH unusually sensitive include: 1) the capture of the whole wave—both the classical amplitude captured by traditional optical systems, and the phase of the wave, with phase potentially measured to ˜1/1000'th of a wavelength or ˜2 to 3 Angstroms for a deep ultra-violet (DUV) laser; 2) heterodyne detection—this allows it to capture very low signal levels; and 3) a head-on geometry using a collimated laser beam that allows best penetration of HAR structures. The basic features and methods of this patented technology are presented, along with simple calculations of signal strength and expected noise levels for various circumstances. Full-wave numerical calculations of electromagnetic field penetration into HAR contacts and experimental results from various wafer types and structures are also presented.

  20. Real-time terahertz digital holography with a quantum cascade laser

    NASA Astrophysics Data System (ADS)

    Locatelli, Massimiliano; Ravaro, Marco; Bartalini, Saverio; Consolino, Luigi; Vitiello, Miriam S.; Cicchi, Riccardo; Pavone, Francesco; de Natale, Paolo

    2015-08-01

    Coherent imaging in the THz range promises to exploit the peculiar capabilities of these wavelengths to penetrate common materials like plastics, ceramics, paper or clothes with potential breakthroughs in non-destructive inspection and quality control, homeland security and biomedical applications. Up to now, however, THz coherent imaging has been limited by time-consuming raster scanning, point-like detection schemes and by the lack of adequate coherent sources. Here, we demonstrate real-time digital holography (DH) at THz frequencies exploiting the high spectral purity and the mW output power of a quantum cascade laser combined with the high sensitivity and resolution of a microbolometric array. We show that, in a one-shot exposure, phase and amplitude information of whole samples, either in reflection or in transmission, can be recorded. Furthermore, a 200 times reduced sensitivity to mechanical vibrations and a significantly enlarged field of view are observed, as compared to DH in the visible range. These properties of THz DH enable unprecedented holographic recording of real world dynamic scenes.

  1. Terahertz in-line digital holography of human hepatocellular carcinoma tissue.

    PubMed

    Rong, Lu; Latychevskaia, Tatiana; Chen, Chunhai; Wang, Dayong; Yu, Zhengping; Zhou, Xun; Li, Zeyu; Huang, Haochong; Wang, Yunxin; Zhou, Zhou

    2015-02-13

    Terahertz waves provide a better contrast in imaging soft biomedical tissues than X-rays, and unlike X-rays, they cause no ionisation damage, making them a good option for biomedical imaging. Terahertz absorption imaging has conventionally been used for cancer diagnosis. However, the absorption properties of a cancerous sample are influenced by two opposing factors: an increase in absorption due to a higher degree of hydration and a decrease in absorption due to structural changes. It is therefore difficult to diagnose cancer from an absorption image. Phase imaging can thus be critical for diagnostics. We demonstrate imaging of the absorption and phase-shift distributions of 3.2 mm × 2.3 mm × 30-μm-thick human hepatocellular carcinoma tissue by continuous-wave terahertz digital in-line holography. The acquisition time of a few seconds for a single in-line hologram is much shorter than that of other terahertz diagnostic techniques, and future detectors will allow acquisition of meaningful holograms without sample dehydration. The resolution of the reconstructions was enhanced by sub-pixel shifting and extrapolation. Another advantage of this technique is its relaxed minimal sample size limitation. The fibrosis indicated in the phase distribution demonstrates the potential of terahertz holographic imaging to obtain a more objective, early diagnosis of cancer.

  2. Digital holography as a method for 3D imaging and estimating the biovolume of motile cells.

    PubMed

    Merola, F; Miccio, L; Memmolo, P; Di Caprio, G; Galli, A; Puglisi, R; Balduzzi, D; Coppola, G; Netti, P; Ferraro, P

    2013-12-07

    Sperm morphology is regarded as a significant prognostic factor for fertilization, as abnormal sperm structure is one of the most common factors in male infertility. Furthermore, obtaining accurate morphological information is an important issue with strong implications in zoo-technical industries, for example to perform sorting of species X from species Y. A challenging step forward would be the availability of a fast, high-throughput and label-free system for the measurement of physical parameters and visualization of the 3D shape of such biological specimens. Here we show a quantitative imaging approach to estimate simply and quickly the biovolume of sperm cells, combining the optical tweezers technique with digital holography, in a single and integrated set-up for a biotechnology assay process on the lab-on-a-chip scale. This approach can open the way for fast and high-throughput analysis in label-free microfluidic based "cytofluorimeters" and prognostic examination based on sperm morphology, thus allowing advancements in reproductive science.

  3. Testing of bioactive-nanovesicles on hepatotoxicity of atypical antipsychotics via digital holography.

    PubMed

    Ozturk Kirbay, Fatma; Geyik, Caner; Guler, Emine; Yesiltepe, Ozan; Gumus, Zinar Pinar; Odaci Demirkol, Dilek; Coskunol, Hakan; Timur, Suna

    2017-04-01

    Atypical antipsychotic drugs induce hepatic toxicity. Thus, it is of importance to eliminate the side effects of these drugs. Herein we describe the preparation of nanoemulsions with a dietary supplement; wheat germ oil (WGO), to ameliorate the liver damage induced by clozapine and olanzapine. THLE-2 cell line was used as a model to investigate the effects of these nanoemulsions on cell viability as well as antioxidative efficiency after antipsychotic insult. In this context, a conventional cell culture method; MTT was used along with a novel cellular imaging technique called digital holography (DH) to evaluate cell viability. Obtained data confirmed that both clozapine and olanzapine induced the liver damage in in vitro model and WGO nanoemulsions were found to be effective on cells and eliminate the cytotoxic effects of these drugs. Briefly, this study has some outputs as follows; it showed that different dietary supplements can be used in such formulations instead of their pristine forms to increase bioavailability. Also, DH was successfully applied for the monitoring of cell viability and it could be a promising approach as the reactive-free cytotoxicity test.

  4. Direct-to-digital holography reduction of reference hologram noise and fourier space smearing

    DOEpatents

    Voelkl, Edgar

    2006-06-27

    Systems and methods are described for reduction of reference hologram noise and reduction of Fourier space smearing, especially in the context of direct-to-digital holography (off-axis interferometry). A method of reducing reference hologram noise includes: recording a plurality of reference holograms; processing the plurality of reference holograms into a corresponding plurality of reference image waves; and transforming the corresponding plurality of reference image waves into a reduced noise reference image wave. A method of reducing smearing in Fourier space includes: recording a plurality of reference holograms; processing the plurality of reference holograms into a corresponding plurality of reference complex image waves; transforming the corresponding plurality of reference image waves into a reduced noise reference complex image wave; recording a hologram of an object; processing the hologram of the object into an object complex image wave; and dividing the complex image wave of the object by the reduced noise reference complex image wave to obtain a reduced smearing object complex image wave.

  5. Overview of techniques applicable to self-interference incoherent digital holography

    PubMed Central

    Hong, J.; Kim, M. K.

    2015-01-01

    Self-interference incoherent digital holography (SIDH) retrieves the complex hologram from the object illuminated by the incoherent light. Supported by the adaptive optic feature, SIDH is readily applicable to the ocular imaging to investigate the human retinal cells. Considering the practical issues, issues related to resolution, phase-shifting, and contrast should be addressed to implement the viable SIDH system which is capable of recording the holographic information of human retinal cells under the incoherent illumination. Super resolution image reconstruction technique can be directly applied to SIDH to enhance the resolution of the system without any change of configuration. We present the improved way to incorporate the phase-shifting itself into the lateral shift required by the super resolution technique. To deal with the phase-shifting issue, we present an arbitrary phase shift retrieval algorithm which can reduce the number of phase-shift and accept the blind phase-shift. The single-shot imaging is also possible by adopting the off-axis configuration of SIDH. We will provide the detailed procedures to retrieve the complex hologram using the proposed arbitrary phase shifting algorithm and the off-axis configuration. PMID:26985327

  6. Overview of Techniques Applicable to Self-Interference Incoherent Digital Holography

    NASA Astrophysics Data System (ADS)

    Hong, J.; Kim, M. K.

    2013-12-01

    Self-interference incoherent digital holography (SIDH) retrieves the complex hologram from the object illuminated by the incoherent light. Supported by the adaptive optic feature, SIDH is readily applicable to the ocular imaging to investigate the human retinal cells. Considering the practical issues, issues related to resolution, phase-shifting, and contrast should be addressed to implement the viable SIDH system which is capable of recording the holographic information of human retinal cells under the incoherent illumination. Super resolution image reconstruction technique can be directly applied to SIDH to enhance the resolution of the system without any change of configuration. We present the improved way to incorporate the phase-shifting itself into the lateral shift required by the super resolution technique. To deal with the phase-shifting issue, we present an arbitrary phase shift retrieval algorithm which can reduce the number of phase-shift and accept the blind phase-shift. The single-shot imaging is also possible by adopting the off-axis configuration of SIDH. We will provide the detailed procedures to retrieve the complex hologram using the proposed arbitrary phase shifting algorithm and the off-axis configuration.

  7. Overview of techniques applicable to self-interference incoherent digital holography.

    PubMed

    Hong, J; Kim, M K

    Self-interference incoherent digital holography (SIDH) retrieves the complex hologram from the object illuminated by the incoherent light. Supported by the adaptive optic feature, SIDH is readily applicable to the ocular imaging to investigate the human retinal cells. Considering the practical issues, issues related to resolution, phase-shifting, and contrast should be addressed to implement the viable SIDH system which is capable of recording the holographic information of human retinal cells under the incoherent illumination. Super resolution image reconstruction technique can be directly applied to SIDH to enhance the resolution of the system without any change of configuration. We present the improved way to incorporate the phase-shifting itself into the lateral shift required by the super resolution technique. To deal with the phase-shifting issue, we present an arbitrary phase shift retrieval algorithm which can reduce the number of phase-shift and accept the blind phase-shift. The single-shot imaging is also possible by adopting the off-axis configuration of SIDH. We will provide the detailed procedures to retrieve the complex hologram using the proposed arbitrary phase shifting algorithm and the off-axis configuration.

  8. Compressive optical image encryption with two-step-only quadrature phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Li, Jun; Li, Hongbing; Li, Jiaosheng; Pan, Yangyang; Li, Rong

    2015-06-01

    An image encryption method which combines two-step-only quadrature phase-shifting digital holography with compressive sensing (CS) has been proposed in the fully optical domain. An object image is firstly encrypted to two on-axis quadrature-phase holograms using the two random phase masks in the Mach-Zehnder interferometer. Then, the two encrypted images are highly compressed to a one-dimensional signal using the single-pixel compressive holographic imaging in the optical domain. At the receiving terminal, the two compressive encrypted holograms are exactly reconstructed from much less than the Nyquist sampling number of observations by solving an optimization problem, and the original image can be decrypted with only two reconstructed holograms and the correct keys. This method largely decreases holograms data volume for the current optical image encryption system, and it is also suitable for some special optical imaging cases such as different wavelengths imaging and weak light imaging. Numerical simulation is performed to demonstrate the feasibility and validity of this novel image encryption method.

  9. Integrated digital holography for measuring the photothermal effect induced by femtosecond laser pulses

    NASA Astrophysics Data System (ADS)

    Zhu, Linwei; Sun, Meiyu; Chen, Jiannong; Yu, Junjie; Zhou, Changhe

    2014-11-01

    Thermal lens (TL) and thermal mirror (TM) effects have been widely used for measuring the thermo-optical properties in materials. However, most previous research is not a direct two-dimensional measurement of the phase difference induced by photothermal effects, and the TL and TM effects cannot be measured simultaneously. We present an integrated digital holography (IDH) for measuring photothermal effects induced by femtosecond laser pulses with the laser excitation fluence below the ablation threshold. The photothermal effects of a metal sample induced by femtosecond laser pulses are studied. Our theoretical analysis reveals that when the energy of the femtosecond laser is below the ablation threshold, the theory of heat conduction and thermoelasticity can be used to explain the TL and TM effects caused by the laser-induced nonuniform temperature distribution. The experimental results show that both the nanoscale surface deformation of the TM effect and the refraction index change of the TL effect can be measured simultaneously by using the IDH. This IDH setup could be suitable for measuring the optical and thermal properties of materials.

  10. Ultrafast axial scanning for two-photon microscopy via a digital micromirror device and binary holography.

    PubMed

    Cheng, Jiyi; Gu, Chenglin; Zhang, Dapeng; Wang, Dien; Chen, Shih-Chi

    2016-04-01

    In this Letter, we present an ultrafast nonmechanical axial scanning method for two-photon excitation (TPE) microscopy based on binary holography using a digital micromirror device (DMD), achieving a scanning rate of 4.2 kHz, scanning range of ∼180  μm, and scanning resolution (minimum step size) of ∼270  nm. Axial scanning is achieved by projecting the femtosecond laser to a DMD programmed with binary holograms of spherical wavefronts of increasing/decreasing radii. To guide the scanner design, we have derived the parametric relationships between the DMD parameters (i.e., aperture and pixel size), and the axial scanning characteristics, including (1) maximum optical power, (2) minimum step size, and (3) scan range. To verify the results, the DMD scanner is integrated with a custom-built TPE microscope that operates at 60 frames per second. In the experiment, we scanned a pollen sample via both the DMD scanner and a precision z-stage. The results show the DMD scanner generates images of equal quality throughout the scanning range. The overall efficiency of the TPE system was measured to be ∼3%. With the high scanning rate, the DMD scanner may find important applications in random-access imaging or high-speed volumetric imaging that enables visualization of highly dynamic biological processes in 3D with submillisecond temporal resolution.

  11. Automatic focus determination through cosine and modified cosine score in digital holography

    NASA Astrophysics Data System (ADS)

    He, Aga; Xiao, Wen; Pan, Feng

    2017-03-01

    An automatic focus determination method in digital holography that utilizes the cosine score (CS) of the inner angle between the vectors that result from adjacent axial reconstructed amplitude images is proposed. This method is based on the fact that the optical field near the focus plane contains more regular features of an object than the defocused region. Further, a modified CS (MCS) autofocusing method is proposed to extend the application range and improve the performance of the proposed method. First, a low-pass filter is designed for the object term holograms in the frequency domain. Second, the standardized Z-scores method is applied along the axis to the amplitude images reconstructed from the filtered hologram. Finally, the MCS of the standardized amplitude images is calculated by an improved cosine-like formula. Next, the focus plane is found at the minimum MCS for different types of objects. This method enables accurate focus determination for all types of objects, as well as for the image region with a slow change or small size, which offers time savings and the precise autofocusing of objects with large longitudinal volumes. The simulations and experimental results on a United States Air Force resolution chart and living cells are presented, illustrating the efficiency and potential of the methods.

  12. On the single point resolution of on-axis digital holography.

    PubMed

    Fournier, Corinne; Denis, Loïc; Fournel, Thierry

    2010-08-01

    On-axis digital holography (DH) is becoming widely used for its time-resolved three-dimensional (3D) imaging capabilities. A 3D volume can be reconstructed from a single hologram. DH is applied as a metrological tool in experimental mechanics, biology, and fluid dynamics, and therefore the estimation and the improvement of the resolution are current challenges. However, the resolution depends on experimental parameters such as the recording distance, the sensor definition, the pixel size, and also on the location of the object in the field of view. This paper derives resolution bounds in DH by using estimation theory. The single point resolution expresses the standard deviations on the estimation of the spatial coordinates of a point source from its hologram. Cramér-Rao lower bounds give a lower limit for the resolution. The closed-form expressions of the Cramér-Rao lower bounds are obtained for a point source located on and out of the optical axis. The influences of the 3D location of the source, the numerical aperture, and the signal-to-noise ratio are studied.

  13. Terahertz in-line digital holography of human hepatocellular carcinoma tissue

    NASA Astrophysics Data System (ADS)

    Rong, Lu; Latychevskaia, Tatiana; Chen, Chunhai; Wang, Dayong; Yu, Zhengping; Zhou, Xun; Li, Zeyu; Huang, Haochong; Wang, Yunxin; Zhou, Zhou

    2015-02-01

    Terahertz waves provide a better contrast in imaging soft biomedical tissues than X-rays, and unlike X-rays, they cause no ionisation damage, making them a good option for biomedical imaging. Terahertz absorption imaging has conventionally been used for cancer diagnosis. However, the absorption properties of a cancerous sample are influenced by two opposing factors: an increase in absorption due to a higher degree of hydration and a decrease in absorption due to structural changes. It is therefore difficult to diagnose cancer from an absorption image. Phase imaging can thus be critical for diagnostics. We demonstrate imaging of the absorption and phase-shift distributions of 3.2 mm × 2.3 mm × 30-μm-thick human hepatocellular carcinoma tissue by continuous-wave terahertz digital in-line holography. The acquisition time of a few seconds for a single in-line hologram is much shorter than that of other terahertz diagnostic techniques, and future detectors will allow acquisition of meaningful holograms without sample dehydration. The resolution of the reconstructions was enhanced by sub-pixel shifting and extrapolation. Another advantage of this technique is its relaxed minimal sample size limitation. The fibrosis indicated in the phase distribution demonstrates the potential of terahertz holographic imaging to obtain a more objective, early diagnosis of cancer.

  14. Combination method of digital holography and gravimetric measurement for assessment of a paint drying process

    NASA Astrophysics Data System (ADS)

    Yokota, Masayuki; Kimoto, Yoshiki

    2013-01-01

    A combination method to study the drying process of paints, based on digital holography and gravimetric measurement, is proposed. The proposed method allows taking holographic measurement in a simultaneous way to compare the results obtained by the reconstructed image changes with gravimetric data. By directly comparing a phase change in the reconstructed images of a paint surface and weight change of the paint film, it is found that a stationary state of the paint surface detected by the phase change occurs in the last stage of solvent evaporation and corresponds to a dry-hard of the paint film. The proposed technique can also analyze dryness of clear coat having no scattering particle using the phase change. It is shown that the present technique can allow us to further investigate not only a film formation of clear coat but also an estimation of specific gravity of solvents by comparing directly the phase change with weight loss due to solvent evaporation in the simultaneous measurement.

  15. High-speed femtosecond laser beam shaping based on binary holography using a digital micromirror device.

    PubMed

    Cheng, Jiyi; Gu, Chenglin; Zhang, Dapeng; Chen, Shih-Chi

    2015-11-01

    In this Letter, we present a digital micromirror device (DMD)-based ultrafast beam shaper, i.e., DUBS. To our knowledge, the DUBS is the first binary laser beam shaper that can generate high-resolution (1140×912 pixels) arbitrary beam modes for femtosecond lasers at a rate of 4.2 kHz; the resolution and pattern rate are limited by the DMD. In the DUBS, the spectrum of the input pulsed laser is first angularly dispersed by a transmission grating and subsequently imaged to a DMD with beam modulation patterns; the transmission grating and a high-reflectivity mirror together compensate the angular dispersion introduced by the DMD. The mode of the output beam is monitored by a CCD camera. In the experiments, the DUBS is programmed to generate four different beam modes, including an Airy beam, Bessel beam, Laguerre-Gaussian (LG) beam, and a custom-designed "peace-dove" beam via the principle of binary holography. To verify the high shaping rate, the Airy beam and LG beam are generated alternately at 4.2 kHz, i.e., the maximum pattern rate of our DMD. The overall efficiency of the DUBS is measured to be 4.7%. With the high-speed and high-resolution beam-shaping capability, the DUBS may find important applications in nonlinear microscopy, optical manipulation, and microscale/nanoscale laser machining, etc.

  16. Quantitative phase analysis through scattering media by depth-filtered digital holography

    NASA Astrophysics Data System (ADS)

    Goebel, Sebastian; Jaedicke, Volker; Koukourakis, Nektarios; Wiethoff, Helge; Adinda-Ougba, Adamou; Gerhardt, Nils C.; Welp, Hubert; Hofmann, Martin R.

    2013-02-01

    Digital holography (DH) is capable of providing three-dimensional topological surface profiles with axial resolutions in the nanometer range. To achieve such high resolutions requires an analysis of the phase information of the reflected light by means of numerical reconstruction methods. Unfortunately, the phase analysis of structures located in scattering media is usually disturbed by interference with reflected light from different depths. In contrast, low-coherence interferometry and optical coherence tomography (OCT) use broadband light sources to investigate the sample with a coherence gate providing tomographic measurements in scattering samples with a poorer depth-resolution of a few micrometers. We propose a new approach that allows recovering the phase information even through scattering media. The approach combines both techniques by creating synthesized interference patterns from scanned spectra. After applying an inverse Fourier transform to each spectrum, we yield three-dimensional depth-resolved images. Subsequently, contributions of photons scattered from unwanted regions are suppressed by depth-filtering. The back-transformed data can be considered as multiple synthesized holograms and the corresponding phase information can be extracted directly from the depthfiltered spectra. We used this approach to record and reconstruct holograms of a reflective surface through a scattering layer. Our results demonstrate a proof-of-principle, as the quantitative phase-profile could be recovered and effectively separated from scattering influences. Moreover, additional processing steps could pave the way to further applications, i.e. spectroscopic analysis.

  17. Discretization of continuous convolution operators for accurate modeling of wave propagation in digital holography.

    PubMed

    Chacko, Nikhil; Liebling, Michael; Blu, Thierry

    2013-10-01

    Discretization of continuous (analog) convolution operators by direct sampling of the convolution kernel and use of fast Fourier transforms is highly efficient. However, it assumes the input and output signals are band-limited, a condition rarely met in practice, where signals have finite support or abrupt edges and sampling is nonideal. Here, we propose to approximate signals in analog, shift-invariant function spaces, which do not need to be band-limited, resulting in discrete coefficients for which we derive discrete convolution kernels that accurately model the analog convolution operator while taking into account nonideal sampling devices (such as finite fill-factor cameras). This approach retains the efficiency of direct sampling but not its limiting assumption. We propose fast forward and inverse algorithms that handle finite-length, periodic, and mirror-symmetric signals with rational sampling rates. We provide explicit convolution kernels for computing coherent wave propagation in the context of digital holography. When compared to band-limited methods in simulations, our method leads to fewer reconstruction artifacts when signals have sharp edges or when using nonideal sampling devices.

  18. A Preliminary Comparison of Three Dimensional Particle Tracking and Sizing using Plenoptic Imaging and Digital In-line Holography [PowerPoint

    SciTech Connect

    Guildenbecher, Daniel Robert; Munz, Elise Dahnke; Farias, Paul Abraham; Thruow, Brian S

    2015-12-01

    Digital in-line holography and plenoptic photography are two techniques for single-shot, volumetric measurement of 3D particle fields. Here we present a preliminary comparison of the two methods by applying plenoptic imaging to experimental configurations that have been previously investigated with digital in-line holography. These experiments include the tracking of secondary droplets from the impact of a water drop on a thin film of water and tracking of pellets from a shotgun. Both plenoptic imaging and digital in-line holography successfully quantify the 3D nature of these particle fields. This includes measurement of the 3D particle position, individual particle sizes, and three-component velocity vectors. For the initial processing methods presented here, both techniques give out-of-plane positional accuracy of approximately 1-2 particle diameters. For a fixed image sensor, digital holography achieves higher effective in-plane spatial resolutions. However, collimated and coherent illumination makes holography susceptible to image distortion through index of refraction gradients, as demonstrated in the shotgun experiments. On the other hand, plenotpic imaging allows for a simpler experimental configuration. Furthermore, due to the use of diffuse, white-light illumination, plenoptic imaging is less susceptible to image distortion in the shotgun experiments. Additional work is needed to better quantify sources of uncertainty, particularly in the plenoptic experiments, as well as develop data processing methodologies optimized for the plenoptic measurement.

  19. Lensless vision system for in-plane positioning of a patterned plate with subpixel resolution.

    PubMed

    Sandoz, Patrick; Jacquot, Maxime

    2011-12-01

    Whereas vision is an efficient way for noncontact sensing of many physical quantities, it assumes a cumbersome imaging system that may be very problematic in confined environments. In such contexts, the design of a compact vision probe can be based on digital holography that is a lensless imaging principle. In this interferometric method, object scenes are reconstructed numerically through wave propagation computations applied to a diffracted optical field recorded as an interferogram. We applied this approach to the visual positioning of a micropatterned glass plate. The pseudoperiodic pattern deposited on the surface is suited for absolute in-plane position determination as well as for fine object-feature interpolation leading to subpixel resolution. Results obtained demonstrate a lateral resolution of 0.1 μm, corresponding to 1/20th of a pixel, from a 150 μm period of the pseudoperiodic pattern and with a demonstrated excursion range of 1.6 cm. In the future, such position encoding could be applied to the backside of standardized sample holders for the easy localization of regions of interest when specimens are transferred from an instrument to another one, for instance in nanotechnology processes.

  20. High-resolution parallel phase-shifting digital holography using a low-resolution phase-shifting array device based on image inpainting.

    PubMed

    Jiao, Shuming; Zou, Wenbin

    2017-02-01

    Parallel phase-shifting digital holography can record high-quality holograms efficiently from fast-moving objects in a dynamic scene. However, a phase-shifting array device with a cell size identical to image sensors is required, which imposes difficulty in practice. This Letter proposes a novel scheme to employ a low-resolution phase-shifting array device to achieve high-resolution parallel phase-shifting digital holography, based on image inpainting performed on incomplete holograms. The experimental results validate the effectiveness of the proposed scheme.

  1. Imaging on the surfaces of an uneven thickness medium based on hybrid phase retrieval with the assistance of off-axis digital holography

    NASA Astrophysics Data System (ADS)

    Wang, Fengpeng; Wang, Dayong; Panezai, Spozmai; Rong, Lu; Wang, Yunxin; Zhao, Jie

    2017-10-01

    A hybrid phase retrieval method with the assistance of off-axis digital holography is proposed for imaging objects on the surfaces of a transparent medium with uneven thickness. The approximate phase distribution is obtained by a constrained optimization approach from the off-axis hologram, and it is used in an iterative procedure for retrieving the complex field of the object from the Gabor hologram. Furthermore, principal component analysis is introduced for compensating for phase aberrations caused by the medium. Numerical simulations and optical experiments were carried out to validate the proposed method. The quality of the reconstructed image is improved remarkably compared to only off-axis digital holography.

  2. Miniaturized multiwavelength digital holography sensor for extensive in-machine tool measurement

    NASA Astrophysics Data System (ADS)

    Seyler, Tobias; Fratz, Markus; Beckmann, Tobias; Bertz, Alexander; Carl, Daniel

    2017-06-01

    In this paper we present a miniaturized digital holographic sensor (HoloCut) for operation inside a machine tool. With state-of-the-art 3D measurement systems, short-range structures such as tool marks cannot be resolved inside a machine tool chamber. Up to now, measurements had to be conducted outside the machine tool and thus processing data are generated offline. The sensor presented here uses digital multiwavelength holography to get 3D-shape-information of the machined sample. By using three wavelengths, we get a large artificial wavelength with a large unambiguous measurement range of 0.5mm and achieve micron repeatability even in the presence of laser speckles on rough surfaces. In addition, a digital refocusing algorithm based on phase noise is implemented to extend the measurement range beyond the limits of the artificial wavelength and geometrical depth-of-focus. With complex wave field propagation, the focus plane can be shifted after the camera images have been taken and a sharp image with extended depth of focus is constructed consequently. With 20mm x 20mm field of view the sensor enables measurement of both macro- and micro-structure (such as tool marks) with an axial resolution of 1 µm, lateral resolution of 7 µm and consequently allows processing data to be generated online which in turn qualifies it as a machine tool control. To make HoloCut compact enough for operation inside a machining center, the beams are arranged in two planes: The beams are split into reference beam and object beam in the bottom plane and combined onto the camera in the top plane later on. Using a mechanical standard interface according to DIN 69893 and having a very compact size of 235mm x 140mm x 215mm (WxHxD) and a weight of 7.5 kg, HoloCut can be easily integrated into different machine tools and extends no more in height than a typical processing tool.

  3. Simultaneous amplitude and phase contrast imaging of burning fuel particle and flame with digital inline holography: Model and verification

    NASA Astrophysics Data System (ADS)

    Wu, Yingchun; Brunel, Marc; Li, Renxian; Lan, Lijuan; Ao, Wen; Chen, Jia; Wu, Xuecheng; Gréhan, Gérard

    2017-09-01

    Three-dimensional (3D) quantitative measurements of reacting multiphase micro-objects are of great interest in fuel particle combustion, which is composed of an inner fuel particle and an outer gas flame. Three models of digital inline holography of this composite fuel particle in the framework of light scattering and diffraction theories have been proposed, and then verified with reported experimental observations. Results show that the dispersed fuel particle and the gas flame distinguish in the reconstructed amplitude and phase contrast images, and reveal that the low extinction efficiency and phase shifting of the highly transparent, weakly scattering gas flame accounts for the difference.

  4. Dual-wavelength phase-shifting digital holography selectively extracting wavelength information from wavelength-multiplexed holograms.

    PubMed

    Tahara, Tatsuki; Mori, Ryota; Kikunaga, Shuhei; Arai, Yasuhiko; Takaki, Yasuhiro

    2015-06-15

    Dual-wavelength phase-shifting digital holography that selectively extracts wavelength information from five wavelength-multiplexed holograms is presented. Specific phase shifts for respective wavelengths are introduced to remove the crosstalk components and extract only the object wave at the desired wavelength from the holograms. Object waves in multiple wavelengths are selectively extracted by utilizing 2π ambiguity and the subtraction procedures based on phase-shifting interferometry. Numerical results show the validity of the proposed technique. The proposed technique is also experimentally demonstrated.

  5. Space-bandwidth extension in parallel phase-shifting digital holography using a four-channel polarization-imaging camera.

    PubMed

    Tahara, Tatsuki; Ito, Yasunori; Xia, Peng; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2013-07-15

    We propose a method for extending the space bandwidth (SBW) available for recording an object wave in parallel phase-shifting digital holography using a four-channel polarization-imaging camera. A linear spatial carrier of the reference wave is introduced to an optical setup of parallel four-step phase-shifting interferometry using a commercially available polarization-imaging camera that has four polarization-detection channels. Then a hologram required for parallel two-step phase shifting, which is a technique capable of recording the widest SBW in parallel phase shifting, can be obtained. The effectiveness of the proposed method was numerically and experimentally verified.

  6. Terahertz in-line digital holography of dragonfly hindwing: amplitude and phase reconstruction at enhanced resolution by extrapolation.

    PubMed

    Rong, Lu; Latychevskaia, Tatiana; Wang, Dayong; Zhou, Xun; Huang, Haochong; Li, Zeyu; Wang, Yunxin

    2014-07-14

    We report here on terahertz (THz) digital holography on a biological specimen. A continuous-wave (CW) THz in-line holographic setup was built based on a 2.52 THz CO(2) pumped THz laser and a pyroelectric array detector. We introduced novel statistical method of obtaining true intensity values for the pyroelectric array detector's pixels. Absorption and phase-shifting images of a dragonfly's hindwing were reconstructed simultaneously from single in-line hologram. Furthermore, we applied phase retrieval routines to eliminate twin image and enhanced the resolution of the reconstructions by hologram extrapolation beyond the detector area. The finest observed features are 35 μm width cross veins.

  7. Wavelength-scanning digital interference holography for tomographic three-dimensional imaging by use of the angular spectrum method.

    PubMed

    Yu, Lingfeng; Kim, Myung K

    2005-08-15

    A tomographic imaging system based on wavelength-scanning digital interference holography is developed by applying the angular spectrum method. Compared to the well-known Fresnel diffraction formula, which is subject to a minimum distance requirement in reconstruction, the angular spectrum method can reconstruct the wave field at any distance from the hologram plane. The new system allows three-dimensional tomographic images to be extracted with an improved signal-to-noise ratio, a more flexible scanning range, and an easier specimen size selection. Experiments are performed to demonstrate the effectiveness of the method.

  8. Two-dimensional mapping of electro-optic phase retardation in lithium niobate crystals by digital holography.

    PubMed

    de Angelis, M; De Nicola, S; Finizio, A; Pierattini, G; Ferraro, P; Grilli, S; Paturzo, M; Sansone, L; Alfieri, D; De Natale, P

    2005-07-01

    We demonstrate accurate two-dimensional mapping of the phase retardation induced by the electro-optic effect in lithium niobate crystals. Off-axis digital holography is used to investigate congruent z-cut crystals. The spatially resolved optical path difference is interferometrically measured while a linearly rising voltage ramp is applied to the crystal. This procedure provides information on the uniformity of crystals' electro-optic properties and offers the ability to detect the presence of defects that is of fundamental importance for reliable processing of photonic devices.

  9. Comparison of passive ranging integral imaging and active imaging digital holography for three-dimensional object recognition.

    PubMed

    Frauel, Yann; Tajahuerce, Enrique; Matoba, Osamu; Castro, Albertina; Javidi, Bahram

    2004-01-10

    We present an overview of three-dimensional (3D) object recognition techniques that use active sensing by interferometric imaging (digital holography) and passive sensing by integral imaging. We describe how each technique can be used to retrieve the depth information of a 3D scene and how this information can then be used for 3D object recognition. We explore various algorithms for 3D recognition such as nonlinear correlation and target distortion tolerance. We also provide a comparison of the advantages and disadvantages of the two techniques.

  10. 3D profile reconstruction of biological sample by in-line image-plane phase-shifting digital microscopic holography

    NASA Astrophysics Data System (ADS)

    Lu, Xiaoxu; Chen, Jianpei; Liu, Shengde; Ma, Zhijian; Zhang, Zhun; Zhong, Liyun

    2012-10-01

    To improve the measuring accuracy is an important research content for digital microscopic holography (DMH) development and application. In this study, we have upgraded application of DMH through the in-line image-plane phase-shifting technique and the image correlation algorithm to reconstruct the 3D profile of a biological sample. Importantly, since this novel DMH system can obtain the phase-shifting hologram with a high ratio of signal to noise conveniently, the reconstructed algorithm of DMH and the compensation operation of the phase aberration are simplified significantly. Moreover, by using the image correlation algorithm, the digital phase mask with high precision also can be obtained easily; thus both the measuring accuracy of DMH and the quality of the reconstructed image are improved significantly. More importantly, this kind of in-line image-plane phase-shifting digital microscopic holography provides a powerful imaging tool to simultaneously reconstruct the amplitude and the phase of the measured object with submicron scale resolution.

  11. Iterative partial phase encoding based on joint fractional Fourier transform correlator adopting phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Wang, Qu; Guo, Qing; Lei, Liang; Zhou, Jinyun

    2014-02-01

    In this paper, digital holography based on two-step phase shifting interference (PSI) was applied to realize the iterative partial phase encoding with joint fractional transform correlator (JFTC). By this security system, the primitive image is encoded in two joint fractional power spectra (JFPS) corresponding to different phase-shifting values. The encrypted image can be deduced directly from the JFPSs by digital means, thus eliminating the noise interference of dc and conjugate terms. JFTC not only relaxes the alignment requirement but also avoids the beam splitting required by traditional holography. In the iterative partial phase encoding, the random phase masks (RPMs) are generated by chaotic mapping, and encoding areas are confined by a sequence of random binary masks. To recover the primitive image, decipher must regenerate the partial RPMs with correct chaotic conditions and perform inverse fractional Fourier transforms with correct orders. The decryption process can be realized by JFTC or by totally digital means. Simulation and experimental results have been presented to test security level and feasibility of the scheme.

  12. Tile-Based Two-Dimensional Phase Unwrapping for Digital Holography Using a Modular Framework.

    PubMed

    Antonopoulos, Georgios C; Steltner, Benjamin; Heisterkamp, Alexander; Ripken, Tammo; Meyer, Heiko

    2015-01-01

    A variety of physical and biomedical imaging techniques, such as digital holography, interferometric synthetic aperture radar (InSAR), or magnetic resonance imaging (MRI) enable measurement of the phase of a physical quantity additionally to its amplitude. However, the phase can commonly only be measured modulo 2π, as a so called wrapped phase map. Phase unwrapping is the process of obtaining the underlying physical phase map from the wrapped phase. Tile-based phase unwrapping algorithms operate by first tessellating the phase map, then unwrapping individual tiles, and finally merging them to a continuous phase map. They can be implemented computationally efficiently and are robust to noise. However, they are prone to failure in the presence of phase residues or erroneous unwraps of single tiles. We tried to overcome these shortcomings by creating novel tile unwrapping and merging algorithms as well as creating a framework that allows to combine them in modular fashion. To increase the robustness of the tile unwrapping step, we implemented a model-based algorithm that makes efficient use of linear algebra to unwrap individual tiles. Furthermore, we adapted an established pixel-based unwrapping algorithm to create a quality guided tile merger. These original algorithms as well as previously existing ones were implemented in a modular phase unwrapping C++ framework. By examining different combinations of unwrapping and merging algorithms we compared our method to existing approaches. We could show that the appropriate choice of unwrapping and merging algorithms can significantly improve the unwrapped result in the presence of phase residues and noise. Beyond that, our modular framework allows for efficient design and test of new tile-based phase unwrapping algorithms. The software developed in this study is freely available.

  13. Tile-Based Two-Dimensional Phase Unwrapping for Digital Holography Using a Modular Framework

    PubMed Central

    Antonopoulos, Georgios C.; Steltner, Benjamin; Heisterkamp, Alexander; Ripken, Tammo; Meyer, Heiko

    2015-01-01

    A variety of physical and biomedical imaging techniques, such as digital holography, interferometric synthetic aperture radar (InSAR), or magnetic resonance imaging (MRI) enable measurement of the phase of a physical quantity additionally to its amplitude. However, the phase can commonly only be measured modulo 2π, as a so called wrapped phase map. Phase unwrapping is the process of obtaining the underlying physical phase map from the wrapped phase. Tile-based phase unwrapping algorithms operate by first tessellating the phase map, then unwrapping individual tiles, and finally merging them to a continuous phase map. They can be implemented computationally efficiently and are robust to noise. However, they are prone to failure in the presence of phase residues or erroneous unwraps of single tiles. We tried to overcome these shortcomings by creating novel tile unwrapping and merging algorithms as well as creating a framework that allows to combine them in modular fashion. To increase the robustness of the tile unwrapping step, we implemented a model-based algorithm that makes efficient use of linear algebra to unwrap individual tiles. Furthermore, we adapted an established pixel-based unwrapping algorithm to create a quality guided tile merger. These original algorithms as well as previously existing ones were implemented in a modular phase unwrapping C++ framework. By examining different combinations of unwrapping and merging algorithms we compared our method to existing approaches. We could show that the appropriate choice of unwrapping and merging algorithms can significantly improve the unwrapped result in the presence of phase residues and noise. Beyond that, our modular framework allows for efficient design and test of new tile-based phase unwrapping algorithms. The software developed in this study is freely available. PMID:26599984

  14. The image quality and resolution limits of phase-shifting digital holography based on the self-imaging effect

    NASA Astrophysics Data System (ADS)

    Siemion, Agnieszka; Ducin, Izabela; Kakarenko, Karol; Makowski, Michał; Siemion, Andrzej; Suszek, Jarosław; Sypek, Maciej; Wojnowski, Dariusz; Kołodziejczyk, Andrzej

    2010-12-01

    A method of a digital holography based on the use of a self-imaging of the phase element is presented and assessed in terms of image quality and resolution. The experimental results of digital hologram acquisition and reconstructions are given for a standard USAF test pattern. The self imaging effect is used in the reference beam of the Mach-Zehnder interferometer in order to project a structured phase modulated beam directly onto the photosensitive matrix of a digital camera. The main advantage of this method is a simple optical setup and the possibility of performing phase-shifting with a single camera exposure. The numerical reconstruction takes advantage of the Talbot effect and does not involve any approximation or interpolation techniques. In order to evaluate the applicative potential of the method, in this work the image quality is checked for various parameters of the optical setup, especially the period of the self-imaging structure and imaging distances.

  15. Singlet oxygen detection in water by means of digital holography and digital holographic tomography

    NASA Astrophysics Data System (ADS)

    Belashov, A. V.; Petrov, N. V.; Semenova, I. V.; Vasyutinskii, O. S.

    2016-04-01

    The paper presents results on singlet oxygen detection in aqueous solutions of a photosensitizer based on the reconstruction of 3D temperature gradients resulting from nonradiative deactivation of excited oxygen molecules. 3D temperature distributions were reconstructed by means of the inverse Abel transformation from a single digital hologram in the case of cylindrically symmetric distribution of the temperature gradient and using holographic tomography algorithm with filtered back projection in the case of nonsymmetrical distribution. Major features of the applied techniques are discussed and results obtained by the two methods are compared.

  16. Differential-interference-contrast digital in-line holography microscopy based on a single-optical-element.

    PubMed

    Zhang, Yuchao; Xie, Changqing

    2015-11-01

    Both digital in-line holography (DIH) and zone plate-based microscopy have received considerable interest as powerful imaging tools. However, the former suffers from a twin-image noise problem. The latter suffers from low efficiency and difficulty in fabrication. Here, we present an effective and efficient phase-contrast imaging approach, named differential-interference-contrast digital in-line holography (DIC-DIH), by using a single optical element to split the incident light into a plane wave and a converging spherical wave and generate a two-dimensional (2D) DIC effect simultaneously. Specifically, to improve image contrast, we present a new single optical element, termed 2D DIC compound photon sieves, by combining two overlaid binary gratings and a compound photon sieve through two logical XOR operations. The proof-of-concept experiments demonstrate that the proposed technique can eliminate the twin-image noise problem and improve image contrast with high efficiency. Additionally, we present an example of the phase-contrast imaging nonuniform thick photoresist development process.

  17. High-speed broadband slight-off digital holography based on simultaneous two-step Gouy phase shifting interferometry

    NASA Astrophysics Data System (ADS)

    Lu, Xiaowei; Xu, Shixiang; Li, Jingzhen; Cai, Yi; Fan, Yupeng

    2016-02-01

    It is well known that phase shift is depended on wavelength of light source by traditional optical path difference (OPD) in free space or wave plate methods. In this paper, we demonstrated a slight-off two-step Gouy phase shifting interferometry which is suitable for broadband digital holography. This technique uses Gouy phase shift to introduce π phase differences between two reference arms. A dual-channel interferometer was employed to monitor the Gouy phase-shifting between two orthogonal polarized references and snap imaging with single shot enabling potential real-time and high-speed imaging capabilities. Theoretical analysis and numerical simulation comparisons between the proposed method and some traditional phase shifting approaches for broadband digital holography were given. Accurate recovers can be obtained in ideal conditions by Gouy, OPD in free space and λ/2 wave plate methods. However, when considering of environment influence, the accuracy of the traditional methods declined rapidly, the recover errors are more than 70 and 20 times higher than our proposed method. The results also show that Gouy method can get consistent phase shift for broadband light source with little influence with wavelength.

  18. Depth position measurement of inhomogeneities in semi-solid organic materials using 3D pulsed digital holography

    NASA Astrophysics Data System (ADS)

    del Socorro Hernández-Montes, María; Mendoza Santoyo, Fernando; Pérez-López, C.

    2006-02-01

    We show experimental results to determine the depth of inhomogeneities such as glass spheres and biological human tumors, in semi-solids organic materials, like gels (phantom), using the non invasive optical technique called 3D Pulsed Digital Holography (PDH). We reported previously that this technique may be used for the detection of biological tissues1. 3D Pulsed Digital Holography allowed us to make a quantitative analysis of the changes that the phantom suffers when it contains inhomogeneities as compared to a phantom that does not have one. The results obtained there showed quite remarkably the internal fault in semi-solids. In here we report early results obtained from three different object illumination positions that gave 3 wrapped phase maps that allowed the calculation of the depth position of the inhomogeneity within the phantom. The optical technique used looks at the phantom surface micro displacement, where measurements are correlated to the z position of the inhomogeneity inside the phantom. Likewise, the technique is able to show the deformation that the material undergoes in x, y, and z.

  19. A method for total noise removal in digital holography based on enhanced grouping and sparsity enhancement filtering

    NASA Astrophysics Data System (ADS)

    Bianco, Vittorio; Memmolo, Pasquale; Paturzo, Melania; Finizio, Andrea; Ferraro, Pietro

    2017-06-01

    In digital holography (DH), the coherent nature of the employed light sources severely degrades the holographic reconstructions due to a mixture of speckle and incoherent additive noise. These can affect both the visual quality in holographic imaging and display, and the accuracy of quantitative phase-contrast reconstructions. Typically, the noise problem is tackled by reducing the illumination coherence, thus the most intuitive way involves the recording of multiple uncorrelated holograms to be incoherently combined. This framework is known as Multi-Look DH (MLDH). However, single shot recordings are highly desirable in DH, and numerical methods are required to go beyond the improvement bound of ML techniques. Among the existing image processing methods, the 3D Block Matching filtering (BM3D) has shown the best performance. Here we present the MLDH-BM3D, a method specifically suitable to filter DH images that combines the two aforementioned strategies to overcome their respective limitations. We demonstrate the effectiveness of this framework in three different experimental situations, i.e. reconstructions of single wavelength holograms and color holograms in the visible region and the challenging case of the Infrared Radiation Digital Holography (IRDH) reconstructions, where a very severe noise degradation occurs.

  20. Holographic pixel super-resolution in portable lensless on-chip microscopy using a fiber-optic array.

    PubMed

    Bishara, Waheb; Sikora, Uzair; Mudanyali, Onur; Su, Ting-Wei; Yaglidere, Oguzhan; Luckhart, Shirley; Ozcan, Aydogan

    2011-04-07

    We report a portable lensless on-chip microscope that can achieve <1 µm resolution over a wide field-of-view of ∼ 24 mm(2) without the use of any mechanical scanning. This compact on-chip microscope weighs ∼ 95 g and is based on partially coherent digital in-line holography. Multiple fiber-optic waveguides are butt-coupled to light emitting diodes, which are controlled by a low-cost micro-controller to sequentially illuminate the sample. The resulting lensfree holograms are then captured by a digital sensor-array and are rapidly processed using a pixel super-resolution algorithm to generate much higher resolution holographic images (both phase and amplitude) of the objects. This wide-field and high-resolution on-chip microscope, being compact and light-weight, would be important for global health problems such as diagnosis of infectious diseases in remote locations. Toward this end, we validate the performance of this field-portable microscope by imaging human malaria parasites (Plasmodium falciparum) in thin blood smears. Our results constitute the first-time that a lensfree on-chip microscope has successfully imaged malaria parasites.

  1. Common-path on-axis Fresnel holography based on a pinhole array plate.

    PubMed

    Zhang, Xin-Ting; Guo, Cheng-Shan

    2015-01-01

    A common-path and on-axis configuration for improving the resolution power of a lensless Fresnel holographic imaging system is proposed. In this configuration, a pinhole array plate (PAP) is inserted between the object and the recording plane. We demonstrated that the complex amplitude of the object wave can be directly extracted from a single Fresnel hologram of the object wave sampled by the PAP, and the numerical aperture of the effective imaging system can be increased because of the diffraction effect of the pinhole array. It may provide one approach for improving the capabilities of digital holography available for a wide range of wavelengths from far-infrared to x-ray and electron beams.

  2. A strong adaptable autofocusing approach of off-axis infrared digital holography under different quality conditions of holograms

    NASA Astrophysics Data System (ADS)

    Liu, Ning; Yang, Chao

    2017-01-01

    In this paper, we present an innovative autofocusing criterion for the reconstruction of infrared digital holograms. This criterion has the advantages of fast, efficient and precision when determining the reconstruction distance of off-axis digital holography. This criterion is a mean-free high frequency calculation process. We focus on the problem of mean value drifting found in previous published methods and design our new approach to solve it. Unlike the previous methods perform well only with high quality holograms, our method is effective for both high and low quality holograms. Even when hologram is degraded by destructive interference, our method still performs well. This method helps to automatically determine the precise reconstruction distance, and we are sure that this technology can be applied in industrial applications in the future.

  3. Three-dimensional displacement measurement for diffuse object using phase-shifting digital holography with polarization imaging camera.

    PubMed

    Kiire, Tomohiro; Nakadate, Suezou; Shibuya, Masato; Yatagai, Toyohiko

    2011-12-01

    The amount of displacement of a diffused object can be measured using phase-shifting digital holography with a polarization imaging camera. Four digital holograms in quadrature are extracted from the polarization imaging camera and used to calculate the phase hologram. Two Fourier transforms of the phase holograms are calculated before and after the displacement of the object. A phase slope is subsequently obtained from the phase distribution of division between the two Fourier transforms. The slope of the phase distribution is proportional to the lateral displacement of the object. The sensitivity is less than one pixel size in the lateral direction of the movement. The longitudinal component of the displacement can be also measured separately from the intercept on the phase axis along the phase distribution of the division between two Fourier transforms of the phase holograms. © 2011 Optical Society of America

  4. Optical sectioning using a digital Fresnel incoherent-holography-based confocal imaging system

    PubMed Central

    Kelner, Roy; Katz, Barak; Rosen, Joseph

    2015-01-01

    We propose a new type of confocal microscope using Fresnel incoherent correlation holography (FINCH). Presented here is a confocal configuration of FINCH using a phase pinhole and point illumination that is able to suppress out-of-focus information from the recorded hologram and hence combine the super-resolution capabilities of FINCH with the sectioning capabilities of confocal microscopy. PMID:26413560

  5. Hard X-ray Fourier Transform Holography Using a Reference Scatterer Fabricated by Electron-Beam-Assisted Chemical-Vapor Deposition

    SciTech Connect

    Suzuki, M.; Isogami, S.; Tsunoda, M.; Takahashi, S.; Ishio, S.

    2011-09-09

    We present a fabrication method for a reference source that is efficient when used for lensless Fourier transform holography. This method produces a reference source that yields high spatial resolution and enhanced signal-to-noise ratio in a Fourier-transformed real-space image, and is particularly useful for Fourier transform holography experiments in the hard x-ray region.

  6. Improvement of the size estimation of 3D tracked droplets using digital in-line holography with joint estimation reconstruction

    NASA Astrophysics Data System (ADS)

    Verrier, N.; Grosjean, N.; Dib, E.; Méès, L.; Fournier, C.; Marié, J.-L.

    2016-04-01

    Digital holography is a valuable tool for three-dimensional information extraction. Among existing configurations, the originally proposed set-up (i.e. Gabor, or in-line holography), is reasonably immune to variations in the experimental environment making it a method of choice for studies of fluid dynamics. Nevertheless, standard hologram reconstruction techniques, based on numerical light back-propagation are prone to artifacts such as twin images or aliases that limit both the quality and quantity of information extracted from the acquired holograms. To get round this issue, the hologram reconstruction as a parametric inverse problem has been shown to accurately estimate 3D positions and the size of seeding particles directly from the hologram. To push the bounds of accuracy on size estimation still further, we propose to fully exploit the information redundancy of a hologram video sequence using joint estimation reconstruction. Applying this approach in a bench-top experiment, we show that it led to a relative precision of 0.13% (for a 60 μm diameter droplet) for droplet size estimation, and a tracking precision of {σx}× {σy}× {σz}=0.15× 0.15× 1~\\text{pixels} .

  7. Mathematical refocusing of images in electronic holography

    SciTech Connect

    Stetson, Karl A.

    2009-07-01

    This paper presents an illustration of mathematical refocusing of images obtained by the HoloFringe300K electronic holography program. The purpose is to demonstrate that this form of electronic holography is equivalent to image-plane, phase-stepped digital holography. The mathematical refocusing method used here differs from those in common use and may have some advantages.

  8. Simulations of lensless imaging in water window

    NASA Astrophysics Data System (ADS)

    Sveda, L.; Pina, L.

    2016-11-01

    A number of soft x-ray / water window laboratory sources is being developed by many groups including the group at CTU. This paper presents simulations and critical parameter estimates for lensless imaging using the laboratory sources, especially the capillary discharge source being developed by our group.1 Water window lensless imaging is demonstrated to be generally feasible with high repetition laboratory sources.

  9. Common-path phase-shifting lensless holographic microscopy.

    PubMed

    Micó, Vicente; García, Javier

    2010-12-01

    We present an approach capable of high-NA imaging in a lensless digital in-line holographic microscopy layout even outside the Gabor's regime. The method is based on spatial multiplexing at the sample plane, allowing a common-path interferometric architecture, where two interferometric beams are generated by a spatial light modulator (SLM) prior to illuminating the sample. The SLM allows phase-shifting interferometry by phase modulation of the SLM diffracted beam. After proper digital processing, the complex amplitude distribution of the diffracted object wavefront is recovered and numerically propagated to image the sample. Experimental results are reported that validate the proposed method.

  10. Multi-illumination Gabor holography recorded in a single camera snap-shot for high-resolution phase retrieval in digital in-line holographic microscopy

    NASA Astrophysics Data System (ADS)

    Sanz, Martin; Picazo-Bueno, Jose A.; Garcia, Javier; Micó, Vicente

    2015-05-01

    In this contribution we introduce MISHELF microscopy, a new concept and design of a lensless holographic microscope based on wavelength multiplexing, single hologram acquisition and digital image processing. The technique which name comes from Multi-Illumination Single-Holographic-Exposure Lensless Fresnel microscopy, is based on the simultaneous illumination and recording of three diffraction patterns in the Fresnel domain. In combination with a novel and fast iterative phase retrieval algorithm, MISHELF microscopy is capable of high-resolution (micron range) phase-retrieved (twin image elimination) biological imaging of dynamic events (video rate recording speed) since it avoids the time multiplexing needed for the in-line hologram sequence recording when using conventional phase-shifting or phase retrieval algorithms. MISHELF microscopy is validated using two different experimental layouts: one using RGB illumination and detection schemes and another using IRRB as illumination while keeping the RGB color camera as detection device. Preliminary experimental results are provided for both experimental layouts using a synthetic object (USAF resolution test target).

  11. Digital holography based on multiwavelength spatial-bandwidth-extended capturing-technique using a reference arm (Multi-SPECTRA).

    PubMed

    Tahara, Tatsuki; Kaku, Toru; Arai, Yasuhiko

    2014-12-01

    Single-shot digital holography based on multiwavelength spatial-bandwidth-extended capturing-technique using a reference arm (Multi-SPECTRA) is proposed. Both amplitude and quantitative phase distributions of waves containing multiple wavelengths are simultaneously recorded with a single reference arm in a single monochromatic image. Then, multiple wavelength information is separately extracted in the spatial frequency domain. The crosstalk between the object waves with different wavelengths is avoided and the number of wavelengths recorded with both a single-shot exposure and no crosstalk can be increased, by a large spatial carrier that causes the aliasing, and/or by use of a grating. The validity of Multi-SPECTRA is quantitatively, numerically, and experimentally confirmed.

  12. Image reconstruction algorithm for recovering high-frequency information in parallel phase-shifting digital holography [Invited].

    PubMed

    Xia, Peng; Shimozato, Yuki; Tahara, Tatsuki; Kakue, Takashi; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2013-01-01

    We propose an image reconstruction algorithm for recovering high-frequency information in parallel phase-shifting digital holography. The proposed algorithm applies three kinds of interpolations and generates three different kinds of object waves. A Fourier transform is applied to each object wave, and the spatial-frequency domain is divided into 3×3 segments for each Fourier-transformed object wave. After that the segment in which interpolation error is the least among the segments having the same address of the segment in the spatial-frequency domain is extracted. The extracted segments are combined to generate an information-enhanced spatial-frequency spectrum of the object wave, and after that the formed spatial-frequency spectrum is inversely Fourier transformed. Then the high-frequency information of the reconstructed image is recovered. The effectiveness of the proposed algorithm was verified by a numerical simulation and an experiment.

  13. Four-step phase-shifting digital holography simultaneously sensing dual-wavelength information using a monochromatic image sensor

    NASA Astrophysics Data System (ADS)

    Tahara, Tatsuki; Mori, Ryota; Arai, Yasuhiko; Takaki, Yasuhiro

    2015-12-01

    Four-step phase-shifting digital holography simultaneously sensing dual-wavelength information from wavelength-multiplexed holograms is presented. Specific phase shifts for respective wavelengths are introduced to remove the crosstalk components and extract only the object wave at the desired wavelength from the holograms. Object waves at multiple wavelengths are selectively extracted by utilizing 2π ambiguity and the subtraction procedures based on phase-shifting interferometry. Two-step phase-shifting interferometry is merged into the procedures to decrease the number of the recorded holograms. The proposed technique is numerically investigated and experimentally demonstrated. The applicability to the case where the number of wavelengths is more than two and possible noise when using two-step phase-shifting interferometry are discussed.

  14. Detection and correction of wavefront errors caused by slight reference tilt in two-step phase-shifting digital holography.

    PubMed

    Xu, Xianfeng; Cai, Luzhong; Gao, Fei; Jia, Yulei; Zhang, Hui

    2015-11-10

    A simple and convenient method, without the need for any additional optical devices and measurements, is suggested to improve the quality of the reconstructed object wavefront in two-step phase-shifting digital holography by decreasing the errors caused by reference beam tilt, which often occurs in practice and subsequently introduces phase distortion in the reconstructed wave. The effects of reference beam tilt in two-step generalized interferometry is analyzed theoretically, showing that this tilt incurs no error either on the extracted phase shift or on the retrieved real object wave amplitude on the recording plane, but causes great deformation of the recovered object wavefront. A corresponding error detection and correction approach is proposed, and the formulas to calculate the tilt angle and correct the wavefront are deduced. A series of computer simulations to find and remove the wavefront errors caused by reference beam tilt demonstrate the effectiveness of this method.

  15. Three-dimensional motion detection of a 20-nm gold nanoparticle using twilight-field digital holography with coherence regulation.

    PubMed

    Goto, Kazufumi; Hayasaki, Yoshio

    2015-07-15

    In the twilight-field method for obtaining interference fringes with high contrast in in-line digital holography, only the intensity of the reference light is regulated to be close to the intensity of the object light, which is the ultra-weak scattered light from a nanoparticle, by using a low-frequency attenuation filter. Coherence of the light also strongly affects the contrast of the interference fringes. High coherence causes a lot of undesired coherent noise, which masks the fringes derived from the nanoparticles. Too-low coherence results in fringes with low contrast and a correspondingly low signal-to-noise ratio. Consequently, proper regulation of the coherence of the light source, in this study the spectral width, improves the minimum detectable size in holographic three-dimensional position measurement of nanoparticles. By using these methods, we were able to measure the position of a gold nanoparticle with a minimum diameter of 20 nm.

  16. Algorithm for reconstructing wide space-bandwidth information in parallel two-step phase-shifting digital holography.

    PubMed

    Tahara, Tatsuki; Shimozato, Yuki; Xia, Peng; Ito, Yasunori; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Matoba, Osamu; Kubota, Toshihiro

    2012-08-27

    We propose an image-reconstruction algorithm of parallel phase-shifting digital holography (PPSDH) which is a technique of single-shot phase-shifting interferometry. In the conventional algorithms in PPSDH, the residual 0th-order diffraction wave and the conjugate images cannot be removed completely and a part of space-bandwidth information is discarded. The proposed algorithm can remove these residual images by modifying the calculation of phase-shifting interferometry and by using Fourier transform technique, respectively. Then, several types of complex amplitudes are derived from a recorded hologram according to the directions in which the neighboring pixels used for carrying out the spatial phase-shifting interferometry are aligned. Several distributions are Fourier-transformed and wide space-bandwidth information of the object wave is obtained by selecting the spectrum among the Fourier-transformed images in each region of the spatial frequency domain and synthesizing a Fourier-transformed image from the spectrum.

  17. Quantitative, three-dimensional imaging of aluminum drop combustion in solid propellant plumes via digital in-line holography.

    PubMed

    Guildenbecher, Daniel R; Cooper, Marcia A; Gill, Walter; Stauffacher, Howard Lee; Oliver, Michael S; Grasser, Thomas W

    2014-09-01

    Burning aluminized propellants eject reacting molten aluminum drops with a broad size distribution. Prior to this work, in situ measurement of the drop size statistics and other quantitative flow properties was complicated by the narrow depth-of-focus of microscopic videography. Here, digital in-line holography (DIH) is demonstrated for quantitative volumetric imaging of the propellant plume. For the first time, to the best of our knowledge, in-focus features, including burning surfaces, drop morphologies, and reaction zones, are automatically measured through a depth spanning many millimeters. By quantifying all drops within the line of sight, DIH provides an order of magnitude increase in the effective data rate compared to traditional imaging. This enables rapid quantification of the drop size distribution with limited experimental repetition.

  18. An optical symmetric cryptographic system with simultaneous encryption and transmission of binary data and secret key by using dual phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Gil, Sang Keun; Jeon, Seok Hee; Jung, Jong Rae

    2013-03-01

    We propose a new optical symmetric cryptographic system with simultaneous encryption and transmission of binary data and secret key by using dual phase-shifting digital holography. Dual phase-shifting digital holography contains two inner and outer interferometers which are used for encrypting data and a secret key at the same time. The technique using dual phase-shifting digital holographic interferometry is efficient because this scheme has an advantage of interference fringe data acquiring time. Binary information data is encrypted by the secret key by applying phase-shifting digital holographic method, and this secret key is also encrypted by phase-shifting digital holographic method and transmitted. Encrypted digital hologram in our method is Fourier transform hologram and is recorded on CCD with 256 gray-level quantized intensities. These encrypted digital holograms are able to be stored by computer and be transmitted over a communication network. With this encrypted digital hologram, the original binary data are decrypted by the same secret key. Simulation results show that the proposed method can be used for a cipher and security system.

  19. Extended ABCD matrix formalism for the description of femtosecond diffraction patterns; application to femtosecond digital in-line holography with anamorphic optical systems.

    PubMed

    Brunel, Marc; Shen, Huanhuan; Coetmellec, Sebastien; Lebrun, Denis

    2012-03-10

    We present a new model to predict diffraction patterns of femtosecond pulses through complex optical systems. The model is based on the extension of an ABCD matrix formalism combined with generalized Huygens-Fresnel transforms (already used in the CW regime) to the femtosecond regime. The model is tested to describe femtosecond digital in-line holography experiments realized in situ through a cylindrical Plexiglas pipe. The model allows us to establish analytical relations that link the holographic reconstruction process to the experimental parameters of the pipe and of the incident beam itself. Simulations and experimental results are in good concordance. Femtosecond digital in-line holography is shown to allow significant coherent noise reduction, and this model will be particularly efficient to describe a wide range of optical geometries. More generally, the model developed can be easily used in any experiment where the knowledge of the precise evolution of femtosecond transverse patterns is required.

  20. Wavefront holoscopy: application of digital in-line holography for the inspection of engraved marks in progressive addition lenses.

    PubMed

    Perucho, Beatriz; Micó, Vicente

    2014-01-01

    Progressive addition lenses (PALs) are engraved with permanent marks at standardized locations in order to guarantee correct centering and alignment throughout the manufacturing and mounting processes. Out of the production line, engraved marks provide useful information about the PAL as well as act as locator marks to re-ink again the removable marks. Even though those marks should be visible by simple visual inspection with the naked eye, engraving marks are often faint and weak, obscured by scratches, and partially occluded and difficult to recognize on tinted or antireflection-coated lenses. Here, we present an extremely simple optical device (named as wavefront holoscope) for visualization and characterization of permanent marks in PAL based on digital in-line holography. Essentially, a point source of coherent light illuminates the engraved mark placed just before a CCD camera that records a classical Gabor in-line hologram. The recorded hologram is then digitally processed to provide a set of high-contrast images of the engraved marks. Experimental results are presented showing the applicability of the proposed method as a new ophthalmic instrument for visualization and characterization of engraved marks in PALs.

  1. Wavefront holoscopy: application of digital in-line holography for the inspection of engraved marks in progressive addition lenses

    NASA Astrophysics Data System (ADS)

    Perucho, Beatriz; Micó, Vicente

    2014-01-01

    Progressive addition lenses (PALs) are engraved with permanent marks at standardized locations in order to guarantee correct centering and alignment throughout the manufacturing and mounting processes. Out of the production line, engraved marks provide useful information about the PAL as well as act as locator marks to re-ink again the removable marks. Even though those marks should be visible by simple visual inspection with the naked eye, engraving marks are often faint and weak, obscured by scratches, and partially occluded and difficult to recognize on tinted or antireflection-coated lenses. Here, we present an extremely simple optical device (named as wavefront holoscope) for visualization and characterization of permanent marks in PAL based on digital in-line holography. Essentially, a point source of coherent light illuminates the engraved mark placed just before a CCD camera that records a classical Gabor in-line hologram. The recorded hologram is then digitally processed to provide a set of high-contrast images of the engraved marks. Experimental results are presented showing the applicability of the proposed method as a new ophthalmic instrument for visualization and characterization of engraved marks in PALs.

  2. Digital holography with multidirectional illumination by LCoS SLM for topography measurement of high gradient reflective microstructures.

    PubMed

    Józwik, Michał; Kozacki, Tomasz; Liżewski, Kamil; Kostencka, Julianna

    2015-03-20

    In this paper we present a method for topography measurement of high gradient reflective microstructures that overcomes the limited numerical aperture (NA) of a digital holographic (DH) system working in reflection. We consider a case when a DH system is unable to register the light reflected from the full sample area due to insufficient NA. To overcome this problem, we propose digital holography in a microscope configuration with an afocal imaging system and a modified object arm in the measurement setup. The proposed modification includes application of a spatial light modulator (SLM) based on liquid crystal on silicon (LCoS) technology for multidirectional plane wave illumination. The variable off-axis illumination enables characterization of the sample regions that cannot be imaged by the limited NA of a classical DH system utilizing on-axis illumination. In the proposed method, the final object topography is merged from a set of captured object waves corresponding to various illumination directions using a novel automatic algorithm. The proposed technique is experimentally validated by full-field measurement of a silicon mold with a high gradient of shape.

  3. Lens-less surface second harmonic imaging

    PubMed Central

    Sly, Krystal L.; Nguyen, Trang T.; Conboy, John C.

    2012-01-01

    Lens-less surface second harmonic generation imaging (SSHGI) is used to image an SHG active molecule, (S)-( + )-1,1’-bi-2-naphthol (SBN), incorporated into a lipid bilayer patterned with the 1951 United States Air Force resolution test target. Data show the coherent plane-wave nature of SHG allows direct imaging without the aid of a lens system. Lens-less SSHGI readily resolves line-widths as small as 223 μm at an object-image distance of 7.6 cm and line-widths of 397 μm at distances as far as 30 cm. Lens-less SSHGI simplifies the detection method, raises photon collection efficiency, and expands the field-of-view. These advantages allow greater throughput and make lens-less SSHGI a potentially valuable detection method for biosensors and medical diagnostics. PMID:23037346

  4. Lens-less surface second harmonic imaging.

    PubMed

    Sly, Krystal L; Nguyen, Trang T; Conboy, John C

    2012-09-24

    Lens-less surface second harmonic generation imaging (SSHGI) is used to image an SHG active molecule, (S)-(+)-1,1'-bi-2-naphthol (SBN), incorporated into a lipid bilayer patterned with the 1951 United States Air Force resolution test target. Data show the coherent plane-wave nature of SHG allows direct imaging without the aid of a lens system. Lens-less SSHGI readily resolves line-widths as small as 223 μm at an object-image distance of 7.6 cm and line-widths of 397 μm at distances as far as 30 cm. Lens-less SSHGI simplifies the detection method, raises photon collection efficiency, and expands the field-of-view. These advantages allow greater throughput and make lens-less SSHGI a potentially valuable detection method for biosensors and medical diagnostics.

  5. Depth enhancement of 3D microscopic living-cell image using incoherent fluorescent digital holography.

    PubMed

    Bang, L T; Wu, H Y; Zhao, Y; Kim, E G; Kim, N

    2017-03-01

    Multilayer images of living cells are typically obtained using confocal or multiphoton microscopy. However, limitations on the distance between consecutive scan layers hinder high-resolution three-dimensional reconstruction, and scattering strongly degrades images of living cell components. Consequently, when overlapping information from different layers is focused on a specific point in the camera, this causes uncertainty in the depiction of the cell components. We propose a method that combines the Fresnel incoherent correlation holography and a depth-of-focus reduction algorithm to enhance the depth information of three-dimensional cell images. The proposed method eliminates overlap between light elements in the different layers inside living cells and limitations on the interlayer distance, and also enhances the contrast of the reconstructed holograms of living cells. © 2016 The Authors Journal of Microscopy © 2016 Royal Microscopical Society.

  6. Dynamic deformation measurements of a rotating disc by twin-pulsed 3D digital holography and interpolation of phase maps

    NASA Astrophysics Data System (ADS)

    Perez-Lopez, Carlos; Mendoza Santoyo, Fernando

    2004-06-01

    This paper describes the application of twin-pulsed 3D digital holography to the measurement of the dynamic deformation of a disc while it rotates. Object rotation produces interferometric fringes that are related to deformations for instance, stress due to the centrifugal forces, out-to plane vibrations, and the object angular displacement. Furthermore an unbalanced disc that rotates may present a characteristic vibration amplitude pattern at a specific frequency. An optical arrangement that illuminates, with a twin pulsed laser, from three different positions the object was used to recover the x, y and z displacement components in a rotating object. The technique is able to distinguish the disc rotation from the displacement along the x-y plane and the out-of-plane z displacement. Two laser pulses are fired in order to take two digital holgrams with a time separation of 20 μs. This is done for each of the three object illumination positions. Triads of twin-pulsed digital holograms taken at different times during object rotation are processed independently, and their optical phase maps retrieved by the conventional Fourier transform method together with the combination of data from the three illumination positions. The phase term related to the deformation is found experimentally where the intrinsic sensitivity vector is related to the rotation via the vector cross product, forming parallel fringes. To recover the rotation and deformation data the unwrapped phase maps were used as 'tilt' phase planes an all three sensitivity vectors in order to recover the in-plane, and out-to plane displacements. An interpolation algorithm was developed to correlate the time depending phase maps, leading to obtain object vibration frequency data. Experimental results are presented, showing in particular that the rotating object has an unbalancing due to the detected vibration frequency.

  7. Digital holography for mechanical vibration measurements in rigid body displacement: elimination of the latter by means of a variable focal length adjustment

    NASA Astrophysics Data System (ADS)

    Pérez-López, Carlos; Mendoza Santoyo, Fernando; Gutiérrez Hernández, David Asael; Muñoz Solis, Silvino

    2008-06-01

    We present our investigation on the separation of mechanical vibrations from rigid body displacements. Pairs of digital holograms acquired between two consecutive time intervals from this type of events produce phase maps that contain both the vibration and rigid body motion information, or even further fully decorrelated phase maps after computer processing. In order to compensate for body displacements, a conjugate object-image experimental arrangement for digital holography is used to measure the mechanical vibrations in a rectangular flat plate. This is achieved by including an extra lens with variable focal length adjustments in front of the typical lens-aperture combination used in the optical head of a digital holographic set up. Out of plane data is obtained from a framed metal plate subjected to a known modal vibration that is also allowed to move perpendicularly to its surface. We will demonstrate that due to the power adjustment of the added lens the angular phase change in the digital hologram from the known object motion allows the separation of the vibration mode at the image plane. The proposed lens addition into a new optical head arrangement in digital holography combined with an a priori knowledge of the rigid body displacement is able to accurately separate the mechanical vibrations making it a promising method in experiments performed under noisy environments. This research suggests the inclusion of adaptive lenses to control the effective focal length when there is a need to separate two distinctive motion types, i.e., vibration from rigid body motion.

  8. Automated image registration in semiconductor industry: a case study in the direct-to-digital holography inspection system

    NASA Astrophysics Data System (ADS)

    Dai, X. L.; Hunt, Martin A.

    2003-05-01

    Automated image registration based on pattern recognition is a critical procedure in many applications of machine vision and is essential for accurate navigation and change detection. In this paper, an overview of the specific applications of image registration in wafer inspection is given, followed by a case study in the application of image registration for direct to digital holography (DDH) wafer inspection. A complete system of novel algorithms for holographic image capable of accepting a variety of data streams as inputs: (1) complex frequency data; (2) complex spatial data; (3) magnitude of data extracted from holograms; (4) phase data extracted from holograms; and (5) intensity-only data. This flexibility facilitates the development of faster, more reliable, and more efficient DDH processing systems, which is important in system optimization and production. In particular, the system enables the use of the full complex wavefront, which contains both reflectance and structural topology information, in the registration process. The added information contained in the wavefront can be utilized for increased robustness and computational efficiency. Both the theory and implementation of the proposed registration system are briefly described within the framework of DDH processing for wafer inspection tasks. Several examples of defect detection and wafer alignment are given with estimates of accuracy and robustness.

  9. Digital in-line holography: 4-D imaging and tracking of micro-structures and organisms in microfluidics and biology

    NASA Astrophysics Data System (ADS)

    Garcia-Sucerquia, J.; Xu, W.; Jericho, S. K.; Jericho, M. H.; Tamblyn, I.; Kreuzer, H. J.

    2006-01-01

    In recent years, in-line holography as originally proposed by Gabor, supplemented with numerical reconstruction, has been perfected to the point at which wavelength resolution both laterally and in depth is routinely achieved with light by using digital in-line holographic microscopy (DIHM). The advantages of DIHM are: (1) simplicity of the hardware (laser- pinhole-CCD camera), (2) magnification is obtained in the numerical reconstruction, (3) maximum information of the 3-D structure with a depth of field of millimeters, (4) changes in the specimen and the simultaneous motion of many species, can be followed in 4-D at the camera frame rate. We present results obtained with DIHM in biological and microfluidic applications. By taking advantage of the large depth of field and the plane-to-plane reconstruction capability of DIHM, we can produce 3D representations of the paths followed by micron-sized objects such as suspensions of microspheres and biological samples (cells, algae, protozoa, bacteria). Examples from biology include a study of the motion of bacteria in a diatom and the track of algae and paramecium. In microfluidic applications we observe micro-channel flow, motion of bubbles in water and evolution in electrolysis. The paper finishes with new results from an underwater version of DIHM.

  10. Spatially-resolved, three-dimensional spray characterization of impinging jets by digital in-line holography

    NASA Astrophysics Data System (ADS)

    Gao, Jian; Rodrigues, Neil; Sojka, Paul; Chen, Jun

    2014-11-01

    The impinging jet injector is a preferred method for the atomization of liquid rocket propellants. The majority of experimental studies in literature are not spatially-resolved due to the limitations of widely available point-wise and two-dimensional (2D) diagnostic techniques such as phase Doppler anemometry (PDA), which requires significant experimental repetitions to give spatially-resolved measurements. In the present study, digital in-line holography (DIH) is used to provide spatially-resolved, three-dimensional (3D) characteristics of impinging jet sprays. A double-exposure DIH setup is configured to measure droplet 3D, three-component velocity as well as the size distribution. The particle information is extracted by the hybrid method, which is recently proposed as a particle detection method. To enlarge the detection volume, two parallel, collimated laser beams are used to simultaneously probe the spray at two locations, and two identical cameras are used to record the corresponding holograms. Such a setup has a detection volume of approximately 20 cm by 3.6 cm by 4.8 cm. Sprays of both Newtonian and non-Newtonian liquids corresponding to regimes at relatively lower jet Reynolds and Weber numbers are investigated. Measurements from DIH are further verified by comparison with experimental data obtained from shadowgraph and PDA. It is revealed that DIH is particularly suitable to provide spatially-resolved, 3D measurements of impinging jet sprays that are not particularly dense.

  11. Reduction of the ringing effect in off-axis digital holography reconstruction from two reconstruction distances based on Talbot effect

    NASA Astrophysics Data System (ADS)

    León-Rodríguez, Miguel; Cordero, Raúl R.; Rayas, Juan A.; Martínez-García, Amalia; Martínez-Gonzalez, Adrián; Labbe, Fernando; Téllez-Quiñones, Alejandro; Flores-Muñoz, Victor

    2015-10-01

    A simple strategy based on wavefront propagation in the Fresnel regime to reduce a ringing effect by using an ideal filter in off-axis digital holography (DH) is presented. In addition, we demonstrate a better focusing capacity by using this ideal filter than Butterworth and Gaussian methods. It also provides a way to increase the visibility of the refocused plane by reducing the influence of the out-of-focus planes. We also use the unique feature of the refocusing capability of DH in the reconstructed and enhanced image, which is obtained from the averaging operation between the image at the focused image plane (z=z) and the first Talbot distance order (z=z). This distance is determined by the periodic ringing. Reductions of 50% of these anomalies are computed in simulation and 30% is obtained experimentally (nearly 2 nm). Also a numerical simulation shows that the focusing resolution is directly related to the filter size and shows a 0.8 mm focus zone with an ideal filter. Numerical simulations and experimental results are carried out to validate the proposal.

  12. Phase correction method for least-squares wavefront calculation in statistical generalized phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Yoshikawa, Nobukazu; Kajihara, Kazuki

    2015-09-01

    When phase-shifting digital holography with a continuous fringe-scanning scheme is implemented using a PC-based measurement system without any synchronous circuit, nonuniform phase-shifted interference fringes are captured because of the fluctuation in the image-capturing interval. To cope with the nonuniform phase shifts, a statistical generalized phase-shifting approach is employed. Because the algorithm is designed to use an arbitrary phase shift, the nonuniform phase shifts do not obstruct object-wave retrieval. Moreover, multiple interference fringes can be obtained in a short time owing to the continuous fringe-scanning scheme. However, the wavefront calculation method is not designed for sequentially recorded interference fringes. To use multiple interference fringes appropriately, we develop a least-squares wavefront calculation method combined with corrections for the initial phase and the direction of phase rotation. We verify the proposed method by numerical simulations and optical experiments. The results show that the object wave with the same initial phase can be correctly reconstructed by using both phase correction methods simultaneously.

  13. Experimental validation for the determination of particle positions by the correlation coefficient method in digital particle holography.

    PubMed

    Yang, Yan; Kang, Bo-seon

    2008-11-10

    The feasibility and the accuracy of the correlation coefficient (CC) method for the determination of particle positions along the optical axis in digital particle holography were verified by validation experiments. A translation system capable of high precision was used to move the particle objects by exact known distances between several different positions. The particle positions along the optical axis were calculated by the CC method and compared with their exact values to obtain the errors of the focus plane determination. The tested particles were two-dimensional (2D) dots in a calibration target along with different-sized glass beads and droplets that reflected and caused a three-dimensional (3D) effect. The results show that the CC method can work well for both the 2D dots and the 3D particles. The effect of other particles on the focus plane determination was also investigated. The CC method can locate the focus plane of particles with high precision, regardless of the existence of other particles.

  14. Quantitative Phase Microscopy of microstructures with extended measurement range and correction of chromatic aberrations by multiwavelength digital holography.

    PubMed

    Ferraro, P; Miccio, L; Grilli, S; Paturzo, M; De Nicola, S; Finizio, A; Osellame, R; Laporta, P

    2007-10-29

    Quantitative Phase Microscopy (QPM) by interferometric techniques can require a multiwavelength configuration to remove 2pi ambiguity and improve accuracy. However, severe chromatic aberration can affect the resulting phase-contrast map. By means of classical interference microscope configuration it is quite unpractical to correct such aberration. We propose and demonstrate that by Digital Holography (DH) in a microscope configuration it is possible to clear out the QPM map from the chromatic aberration in a simpler and more effective way with respect to other approaches. The proposed method takes benefit of the unique feature of DH to record in a plane out-of-focus and subsequently reconstruct numerically at the right focal image plane. In fact, the main effect of the chromatic aberration is to shift differently the correct focal image plane at each wavelength and this can be readily compensated by adjusting the corresponding reconstruction distance for each wavelength. A procedure is described in order to determine easily the relative focal shift among different imaging wavelengths by performing a scanning of the numerical reconstruction along the optical axis, to find out the focus and to remove at the same time the chromatic aberration.

  15. Lensless single-exposure super-resolved interferometric microscopy

    NASA Astrophysics Data System (ADS)

    Granero, Luis; Ferreira, Carlos; García, Javier; Micó, Vicente

    2013-04-01

    Single Exposure Super Resolved Interferometric Microscopy (SESRIM) has been recently proposed as a way to achieve one dimensional super resolved imaging in digital holographic microscopy. SESRIM uses Red-Green-Blue (RGB) multiplexing for illuminating the sample having different propagation angles for each one of the three illumination wavelengths and it has been experimentally validated considering color (A. Calabuig, V. Mico, J. Garcia, Z. Zalevsky, and C. Ferreira, "Single-exposure super-resolved interferometric microscopy by red-green-blue multiplexing," Opt. Lett. 36, 885-887, 2011) and monochrome (A. Calabuig, J. Garcia, C. Ferreira, Z. Zalevsky, and V. Mico, "Resolution improvement by single-exposure superresolved interferometric microscopy with a monochrome sensor," J. Opt. Soc. Am. A 28, 2346-2358, 2011) digital sensors for holographic recording. In this contribution, we will first review some of the characteristics of the previously reported SESRIM approaches and second, we will present preliminary results for the extension of SESRIM to the field of lensless holographic microscopy. Experimental results are reported validating this new kind of superresolution imaging method named as lensless SESRIM (L-SESRIM).

  16. Ultra-fast digital holography of the femto-second order

    NASA Astrophysics Data System (ADS)

    Zhai, Hongchen; Wang, Xiaolei; Mu, Guoguang

    2007-01-01

    We report on pulsed digital micro holographic systems recording ultra-fast process of the femto-second order, by spatially angular division multiplexing (SADM) and wavelength division multiplexing (WDM), respectively. Both intensity and phase images of the digitally reconstructed images are obtained through Fourier transformation and digital filtering, which show clearly the plasma forming and propagating dynamic process of laser induced ionization of ambient air at the wavelength of 800 nm, with a time resolution of 50 fs and frame intervals of 300 to 550 fs.

  17. Thin wetting film lensless imaging

    NASA Astrophysics Data System (ADS)

    Allier, C. P.; Poher, V.; Coutard, J. G.; Hiernard, G.; Dinten, J. M.

    2011-03-01

    Lensless imaging has recently attracted a lot of attention as a compact, easy-to-use method to image or detect biological objects like cells, but failed at detecting micron size objects like bacteria that often do not scatter enough light. In order to detect single bacterium, we have developed a method based on a thin wetting film that produces a micro-lens effect. Compared with previously reported results, a large improvement in signal to noise ratio is obtained due to the presence of a micro-lens on top of each bacterium. In these conditions, standard CMOS sensors are able to detect single bacterium, e.g. E.coli, Bacillus subtilis and Bacillus thuringiensis, with a large signal to noise ratio. This paper presents our sensor optimization to enhance the SNR; improve the detection of sub-micron objects; and increase the imaging FOV, from 4.3 mm2 to 12 mm2 to 24 mm2, which allows the detection of bacteria contained in 0.5μl to 4μl to 10μl, respectively.

  18. Lensless fluorescent microscopy on a chip.

    PubMed

    Coskun, Ahmet F; Su, Ting-Wei; Sencan, Ikbal; Ozcan, Aydogan

    2011-08-17

    On-chip lensless imaging in general aims to replace bulky lens-based optical microscopes with simpler and more compact designs, especially for high-throughput screening applications. This emerging technology platform has the potential to eliminate the need for bulky and/or costly optical components through the help of novel theories and digital reconstruction algorithms. Along the same lines, here we demonstrate an on-chip fluorescent microscopy modality that can achieve e.g., <4 μm spatial resolution over an ultra-wide field-of-view (FOV) of >0.6-8 cm(2) without the use of any lenses, mechanical-scanning or thin-film based interference filters. In this technique, fluorescent excitation is achieved through a prism or hemispherical-glass interface illuminated by an incoherent source. After interacting with the entire object volume, this excitation light is rejected by total-internal-reflection (TIR) process that is occurring at the bottom of the sample micro-fluidic chip. The fluorescent emission from the excited objects is then collected by a fiber-optic faceplate or a taper and is delivered to an optoelectronic sensor array such as a charge-coupled-device (CCD). By using a compressive-sampling based decoding algorithm, the acquired lensfree raw fluorescent images of the sample can be rapidly processed to yield e.g., <4 μm resolution over an FOV of >0.6-8 cm(2). Moreover, vertically stacked micro-channels that are separated by e.g., 50-100 μm can also be successfully imaged using the same lensfree on-chip microscopy platform, which further increases the overall throughput of this modality. This compact on-chip fluorescent imaging platform, with a rapid compressive decoder behind it, could be rather valuable for high-throughput cytometry, rare-cell research and microarray-analysis.

  19. Pulsed digital holography system recording ultrafast process of the femtosecond order

    NASA Astrophysics Data System (ADS)

    Wang, Xiaolei; Zhai, Hongchen; Mu, Guoguang

    2006-06-01

    We report, for the first time to our knowledge, a pulsed digital microholographic system with spatial angular multiplexing for recording the ultrafast process of the femtosecond order. The optimized design of the two sets of subpulse-train generators in this system makes it possible to implement a digital holographic recording with spatial angular multiplexing of a frame interval of the femtosecond order, while keeping the incident angle of the object beams unchanged. Three pairs of amplitude and phase images from the same view angle digitally reconstructed by the system demonstrated the ultrafast dynamic process of laser-induced ionization of ambient air at a wavelength of 800 nm, with a time resolution of 50 fs and a frame interval of 300 fs.

  20. Single-shot digital holography by use of the fractional Talbot effect.

    PubMed

    Martínez-León, Lluís; Araiza-E, María; Javidi, Bahram; Andrés, Pedro; Climent, Vicent; Lancis, Jesús; Tajahuerce, Enrique

    2009-07-20

    We present a method for recording in-line single-shot digital holograms based on the fractional Talbot effect. In our system, an image sensor records the interference between the light field scattered by the object and a properly codified parallel reference beam. A simple binary two-dimensional periodic grating is used to codify the reference beam generating a periodic three-step phase distribution over the sensor plane by fractional Talbot effect. This provides a method to perform single-shot phase-shifting interferometry at frame rates only limited by the sensor capabilities. Our technique is well adapted for dynamic wavefront sensing applications. Images of the object are digitally reconstructed from the digital hologram. Both computer simulations and experimental results are presented.

  1. Spatial-carrier phase-shifting digital holography utilizing spatial frequency analysis for the correction of the phase-shift error.

    PubMed

    Tahara, Tatsuki; Shimozato, Yuki; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Matoba, Osamu; Kubota, Toshihiro

    2012-01-15

    We propose a single-shot digital holography in which the complex amplitude distribution is obtained by spatial-carrier phase-shifting (SCPS) interferometry and the correction of the inherent phase-shift error occurred in this interferometry. The 0th order diffraction wave and the conjugate image are removed by phase-shifting interferometry and Fourier transform technique, respectively. The inherent error is corrected in the spatial frequency domain. The proposed technique does not require an iteration process to remove the unwanted images and has an advantage in the field of view in comparison to a conventional SCPS technique.

  2. Digital holography and quantitative phase contrast imaging using computational shear interferometry

    NASA Astrophysics Data System (ADS)

    Falldorf, Claas; Agour, Mostafa; Bergmann, Ralf B.

    2015-02-01

    In this publication, we give a brief introduction into the field of computational shear interferometry, which allows for determining arbitrary wave fields from a set of shear interferograms. We discuss limitations of the method with respect to the coherence of the underlying wave field and present various numerical methods to recover it from its sheared representations. Finally, we show experimental results on digital holography of objects with rough surfaces using a fiber coupled light-emitting diode and quantitative phase contrast imaging as well as numerical refocusing in differential interference contrast microscopy.

  3. Single-shot color digital holography based on the fractional Talbot effect.

    PubMed

    Araiza-Esquivel, María A; Martínez-León, Lluís; Javidi, Bahram; Andrés, Pedro; Lancis, Jesús; Tajahuerce, Enrique

    2011-03-01

    We present a method for recording on-axis color digital holograms in a single shot. Our system performs parallel phase-shifting interferometry by using the fractional Talbot effect for every chromatic channel simultaneously. A two-dimensional binary amplitude grating is used to generate Talbot periodic phase distributions in the reference beam. The interference patterns corresponding to the three chromatic channels are captured at once at different axial distances. In this scheme, one-shot recording and digital reconstruction allow for real-time measurement. Computer simulations and experimental results confirm the validity of our method. © 2011 Optical Society of America

  4. Digital holography for observing aerosol particles undergoing Brownian motion in microgravity conditions

    NASA Astrophysics Data System (ADS)

    Prodi, F.; Santachiara, G.; Travaini, S.; Belosi, F.; Vedernikov, A.; Dubois, F.; Queeckers, P.; Legros, J. C.

    2006-11-01

    Brownian diffusion of aerosol particles was studied in microgravity conditions using a digital holographic velocimeter. Based on digital image processing, the observed volume, recorded on a charge-coupled device (CCD) camera, is reconstructed slice by slice in order to achieve a full focused volume. Three dimensional coordinates of the particles are retrieved by such procedures and particle trajectories are reconstructed by analysing the sequence of the particle position. We deduced that the displacement of particles in microgravity, due to Brownian motion, follows a Gaussian distribution, like at 1 g. Particle sizes obtained from SEM measurements were in good agreement with those calculated from the three dimensional trajectories provided by the holographic microscope.

  5. Speckle noise reduction in digital holography by slightly rotating the object

    NASA Astrophysics Data System (ADS)

    Herrera-Ramirez, Jorge; Hincapie-Zuluaga, Diego Andrés; Garcia-Sucerquia, Jorge

    2016-12-01

    This work shows the realization of speckle reduction in the numerical reconstruction of digitally recorded holograms by the superposition of multiple slightly rotated digital holographic images of the object. The superposition of T uncorrelated holographic images reduces the contrast of the speckle noise of the image following the expected 1/√{T} law. The effect of the method on the borders of the resulting image is evaluated by quantifying the utilization of the dynamic range or the contrast between the white and black areas of a regular die. Experimental results validate the feasibility of the proposed method.

  6. Study of aluminum particle combustion in solid propellant plumes using digital in-line holography and imaging pyrometry

    DOE PAGES

    Chen, Yi; Guildenbecher, Daniel R.; Hoffmeister, Kathryn N. G.; ...

    2017-05-05

    The combustion of molten metals is an important area of study with applications ranging from solid aluminized rocket propellants to fireworks displays. Our work uses digital in-line holography (DIH) to experimentally quantify the three-dimensional position, size, and velocity of aluminum particles during combustion of ammonium perchlorate (AP) based solid-rocket propellants. Additionally, spatially resolved particle temperatures are simultaneously measured using two-color imaging pyrometry. To allow for fast characterization of the properties of tens of thousands of particles, automated data processing routines are proposed. In using these methods, statistics from aluminum particles with diameters ranging from 15 to 900 µm are collectedmore » at an ambient pressure of 83 kPa. In the first set of DIH experiments, increasing initial propellant temperature is shown to enhance the agglomeration of nascent aluminum at the burning surface, resulting in ejection of large molten aluminum particles into the exhaust plume. The resulting particle number and volume distributions are quantified. In the second set of simultaneous DIH and pyrometry experiments, particle size and velocity relationships as well as temperature statistics are explored. The average measured temperatures are found to be 2640 ± 282 K, which compares well with previous estimates of the range of particle and gas-phase temperatures. The novel methods proposed here represent new capabilities for simultaneous quantification of the joint size, velocity, and temperature statistics during the combustion of molten metal particles. The proposed techniques are expected to be useful for detailed performance assessment of metalized solid-rocket propellants.« less

  7. Optical encryption of gray-level image using on-axis and 2-f digital holography with two-step phase-shifting method

    NASA Astrophysics Data System (ADS)

    Jeon, Seok Hee; Hwang, Young Gwan; Gil, Sang Keun

    2008-07-01

    In this paper we propose an encryption/decryption technique of gray-level image information using an on-axis 2-f digital holographic optical encrypting system with two-step phase-shifting method. This technique reduces the number of holograms in phase-shifting digital holography and minimizes the setup of the encryption system more than multistep phase-shifting technique. We are able to get the complete decrypted image by controlling the K-ratio which is defined as the reference beam intensity versus the object beam intensity. We remove the DC-term of the phase-shifting digital hologram to reconstruct and decrypt the original image information. Simulation results show that the proposed method can be used for encryption and decryption of a 256 gray-level image. Also, the result shows some errors of the decrypted image according to K-ratio.

  8. Real-time quantitative phase reconstruction in off-axis digital holography using multiplexing.

    PubMed

    Girshovitz, Pinhas; Shaked, Natan T

    2014-04-15

    We present a new approach for obtaining significant speedup in the digital processing of extracting unwrapped phase profiles from off-axis digital holograms. The new technique digitally multiplexes two orthogonal off-axis holograms, where the digital reconstruction, including spatial filtering and two-dimensional phase unwrapping on a decreased number of pixels, can be performed on both holograms together, without redundant operations. Using this technique, we were able to reconstruct, for the first time to our knowledge, unwrapped phase profiles from off-axis holograms with 1 megapixel in more than 30 frames per second using a standard single-core personal computer on a MATLAB platform, without using graphic-processing-unit programming or parallel computing. This new technique is important for real-time quantitative visualization and measurements of highly dynamic samples and is applicable for a wide range of applications, including rapid biological cell imaging and real-time nondestructive testing. After comparing the speedups obtained by the new technique for holograms of various sizes, we present experimental results of real-time quantitative phase visualization of cells flowing rapidly through a microchannel.

  9. Difference displacement measurement by digital holography by use of simulated wave fronts.

    PubMed

    Gombköto, Balázs; Kornis, János; Füzessy, Zoltán; Kiss, Márta; Kovács, Péter

    2004-03-10

    A concept called fringe compensation was first presented in phase-shifting electronic speckle-pattern interferometry. We apply a similar principle to digital holographic interferometry; here the phase of a wave front is known and can be manipulated. The basic mathematical formulation of fringe compensation and some experimental results are shown with relatively large, simple rigid-body rotation and circular membrane deformation.

  10. Contributions to digital holography for the three-dimensional display of medical imagery

    SciTech Connect

    Perlmutter, R.J.

    1983-01-01

    In light of the growing presence and power of the digital computer in the radiology laboratory, a digital holographic approach to the 3-D display of medical image data is investigated. The fundamental concepts involve: producing the digital equivalent of an optical model of the data as a collection of parallel cross-sections; computing the complex Fresnel diffraction patterns (FDP) of the individual cross-sections at the hologram plane and, invoking linearity, summing them to yield the FDP of the 3-D model; modulating a high spatial frequency carrier with the FDP; and recording the resulting sample values on film. This holographic approach provides a volume filling, 3-D image, offering horizontal and vertical parallax, accommodation, and steropsis. It is assumed that the digital data to be displayed has been processed to represent the pertinent information. The data points are modeled as coherently radiating sources. If the holographic image subtends more than a few tenths of a degree the FDP must be sampled at typically 50 times the image sampling rate. Since the hologram forms the viewing window we must compute at least a factor of 50/sup 2/ more FDP sample points than image points to provide a reasonable look-around angle. The computation involved is the 2-D convolution of the coarsely sampled image cross-section and the 2-D quadratic phase function. An algorithm was derived which partitions this huge convolution into a multitude of manageable blocks, each of which can be independently computed with a single 2-D discrete Fourier transform. The advantages are savings in memory, computational costs, time and the suitability for parallel-processor-architecture computer implementation. The mathematical framework was established for a new scanned digital holographic recorder.

  11. Noise suppression of the reconstruction of infrared digital holography based on pyramid-based bilateral filter

    NASA Astrophysics Data System (ADS)

    Liu, Ning; Yang, Chao; Cao, Haijie

    2017-09-01

    In this paper, we present a new technique of speckle noise suppressing of infrared digital hologram. This technique uses the Laplacian pyramid to separate the original reconstructed hologram into different layers, and conducts the bilateral filter onto each layer to discriminate the noise and the detail information. Because not all noise can be discriminated by the bilateral filter, we design an improved median filter to filter the leftover noise. The detail information of each pyramid layer can then be enhanced without too much noise. We retrieve the separated layer back to a reconstructed image using the reverse Laplacian pyramid method. This technique works effectively on the infrared digital holograms in noise suppressing. No multiple holograms or specific mechanical equipment are needed for doing so, which makes this technique easy to calculate and realize. Figures and numerical results are shown in this paper to demonstrate the performance of this technique.

  12. Volumetric three-dimensional recognition of biological microorganisms using multivariate statistical method and digital holography.

    PubMed

    Moon, Inkyu; Javidi, Bahram

    2006-01-01

    We present a new statistical approach to real-time sensing and recognition of microorganisms using digital holographic microscopy. We numerically produce many section images at different depths along a longitudinal direction from the single digital hologram of three-dimensional (3D) microorganisms in the Fresnel domain. For volumetric 3D recognition, the test pixel points are randomly selected from the section image; this procedure can be repeated with different specimens of the same microorganism. The multivariate joint density functions are calculated from the pixel values of each section image at the same random pixel points. The parameters of the statistical distributions are compared using maximum likelihood estimation and statistical inference algorithms. The performance of the proposed system is illustrated with preliminary experimental results.

  13. Automated Three-Dimensional Microbial Sensing and Recognition Using Digital Holography and Statistical Sampling

    PubMed Central

    Moon, Inkyu; Yi, Faliu; Javidi, Bahram

    2010-01-01

    We overview an approach to providing automated three-dimensional (3D) sensing and recognition of biological micro/nanoorganisms integrating Gabor digital holographic microscopy and statistical sampling methods. For 3D data acquisition of biological specimens, a coherent beam propagates through the specimen and its transversely and longitudinally magnified diffraction pattern observed by the microscope objective is optically recorded with an image sensor array interfaced with a computer. 3D visualization of the biological specimen from the magnified diffraction pattern is accomplished by using the computational Fresnel propagation algorithm. For 3D recognition of the biological specimen, a watershed image segmentation algorithm is applied to automatically remove the unnecessary background parts in the reconstructed holographic image. Statistical estimation and inference algorithms are developed to the automatically segmented holographic image. Overviews of preliminary experimental results illustrate how the holographic image reconstructed from the Gabor digital hologram of biological specimen contains important information for microbial recognition. PMID:22163664

  14. Automated three-dimensional microbial sensing and recognition using digital holography and statistical sampling.

    PubMed

    Moon, Inkyu; Yi, Faliu; Javidi, Bahram

    2010-01-01

    We overview an approach to providing automated three-dimensional (3D) sensing and recognition of biological micro/nanoorganisms integrating Gabor digital holographic microscopy and statistical sampling methods. For 3D data acquisition of biological specimens, a coherent beam propagates through the specimen and its transversely and longitudinally magnified diffraction pattern observed by the microscope objective is optically recorded with an image sensor array interfaced with a computer. 3D visualization of the biological specimen from the magnified diffraction pattern is accomplished by using the computational Fresnel propagation algorithm. For 3D recognition of the biological specimen, a watershed image segmentation algorithm is applied to automatically remove the unnecessary background parts in the reconstructed holographic image. Statistical estimation and inference algorithms are developed to the automatically segmented holographic image. Overviews of preliminary experimental results illustrate how the holographic image reconstructed from the Gabor digital hologram of biological specimen contains important information for microbial recognition.

  15. Characterization of atomization and breakup of acoustically levitated drops with digital holography.

    PubMed

    Yao, Longchao; Wu, Xuecheng; Wu, Yingchun; Yang, Jing; Gao, Xiang; Chen, Linghong; Gréhan, Gérard; Cen, Kefa

    2015-01-01

    A digital holographic particle tracking velocimetry system is applied to quantitatively study the drop atomization induced by capillary waves, and the breakup caused by increased sound pressure levels. A wavelet-based algorithm is used for particle detection and autofocusing with a wide size range of 20 μm-2 mm. To eliminate the influence of large particles on small particles, a two-step detection method is adopted. Large drops are first characterized and simulated by a diffraction-based model. Then the contributions of the drops are subtracted from the original hologram followed by the detection of small droplets. Finally, the velocity and size distribution of the secondary droplets are obtained from the experimental holograms. The results demonstrate the validity of the digital in-line holographic technique for the atomization and breakup study of acoustically levitated drops.

  16. Digital holography particle image velocimetry for the measurement of 3D t-3c flows

    NASA Astrophysics Data System (ADS)

    Shen, Gongxin; Wei, Runjie

    2005-10-01

    In this paper a digital in-line holographic recording and reconstruction system was set up and used in the particle image velocimetry for the 3D t-3c (the three-component (3c), velocity vector field measurements in a three-dimensional (3D), space field with time history ( t)) flow measurements that made up of the new full-flow field experimental technique—digital holographic particle image velocimetry (DHPIV). The traditional holographic film was replaced by a CCD chip that records instantaneously the interference fringes directly without the darkroom processing, and the virtual image slices in different positions were reconstructed by computation using Fresnel-Kirchhoff integral method from the digital holographic image. Also a complex field signal filter (analyzing image calculated by its intensity and phase from real and image parts in fast fourier transform (FFT)) was applied in image reconstruction to achieve the thin focus depth of image field that has a strong effect with the vertical velocity component resolution. Using the frame-straddle CCD device techniques, the 3c velocity vector was computed by 3D cross-correlation through space interrogation block matching through the reconstructed image slices with the digital complex field signal filter. Then the 3D-3c-velocity field (about 20 000 vectors), 3D-streamline and 3D-vorticiry fields, and the time evolution movies (30 field/s) for the 3D t-3c flows were displayed by the experimental measurement using this DHPIV method and techniques.

  17. Suppression of the zero-order term in off-axis digital holography through nonlinear filtering.

    PubMed

    Pavillon, Nicolas; Seelamantula, Chandra Sekhar; Kühn, Jonas; Unser, Michael; Depeursinge, Christian

    2009-12-01

    We present experimental validation of a new reconstruction method for off-axis digital holographic microscopy (DHM). This method effectively suppresses the object autocorrelation, namely, the zero-order term, from holographic data, thereby improving the reconstruction bandwidth of complex wavefronts. The algorithm is based on nonlinear filtering and can be applied to standard DHM setups with realistic recording conditions. We study the robustness of the technique under different experimental configurations, and quantitatively demonstrate its enhancement capabilities on phase signals.

  18. An encryption scheme based on phase-shifting digital holography and amplitude-phase disturbance

    NASA Astrophysics Data System (ADS)

    Hua, Li-Li; Xu, Ning; Yang, Geng

    2014-06-01

    In this paper, we propose an encryption scheme based on phase-shifting digital interferometry. According to the original system framework, we add a random amplitude mask and replace the Fourier transform by the Fresnel transform. We develop a mathematical model and give a discrete formula based on the scheme, which makes it easy to implement the scheme in computer programming. The experimental results show that the improved system has a better performance in security than the original encryption method. Moreover, it demonstrates a good capability of anti-noise and anti-shear robustness.

  19. Parallel phase-shifting self-interference digital holography with faithful reconstruction using compressive sensing

    NASA Astrophysics Data System (ADS)

    Wan, Yuhong; Man, Tianlong; Wu, Fan; Kim, Myung K.; Wang, Dayong

    2016-11-01

    We present a new self-interference digital holographic approach that allows single-shot capturing three-dimensional intensity distribution of the spatially incoherent objects. The Fresnel incoherent correlation holographic microscopy is combined with parallel phase-shifting technique to instantaneously obtain spatially multiplexed phase-shifting holograms. The compressive-sensing-based reconstruction algorithm is implemented to reconstruct the original object from the under sampled demultiplexed holograms. The scheme is verified with simulations. The validity of the proposed method is experimentally demonstrated in an indirectly way by simulating the use of specific parallel phase-shifting recording device.

  20. Direct to Digital Holography for Semiconductor Wafer Defect Detection and Review

    SciTech Connect

    ThomasJr., C. E.; Bahm, Tracy M.; Baylor, Larry R; Bingham, Philip R.; Burns, Steven W.; Chidley, Matthew D; Dai, Xiaolong; Delahanty, Robert J.; Doti, Christopher J.; El-Khashab, Ayman; Fisher, Robert L.; Gilbert, Judd M.; Cui, Hongtao; Goddard Jr, James Samuel; Hanson, Gregory R; Hickson, Joel D.; Hunt, Martin A.; Hylton, Kathy W; John, George C.; Jones, Michael L.; McDonald, Kenneth R.; Mayo, Michael W.; McMackin, Ian; Patek, David; Price, John H.; Rasmussen, David A; Schaefer, Louis J.; Scheidt, Thomas R.; Schulze, Mark A.; Schumaker, Philip D.; Shen, Bichuan; Smith, Randall G.; Su, Allen N.; Tobin Jr, Kenneth William; Usry, William R.; Voelkl, Edgar; Weber, Karsten S.; Jones, Paul G.; Owen, Robert W.

    2002-01-01

    A method for recording true holograms (not holographic interferometry) directly to a digital video medium in a single image has been invented. This technology makes the amplitude and phase for every pixel of the target object wave available. Since phase is proportional to wavelength, this makes high-resolution metrology an implicit part of the holographic recording. Measurements of phase can be made to one hundredth or even one thousandth of a wavelength, so the technology is attractive for finding defects on semiconductor wafers, where feature sizes are now smaller than the wavelength of even deep ultra-violet light.