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Sample records for lensless digital holography

  1. Synthetic aperture engineering for super-resolved microscopy in digital lensless Fourier holography

    NASA Astrophysics Data System (ADS)

    Micó, Vicente; Granero, Luis; Zalevsky, Zeev; García, Javier

    2011-05-01

    We present a method capable to improve the resolution limit of an imaging system in digital lensless Fourier holographic configuration. The method is based on angular- and time-multiplexing of the object's spatial frequency information. On one hand, angular multiplexing is implemented by using tilted beam illumination to get access to high order spectral frequency bands of the of the object's spectrum. And, on the other hand, time multiplexing is needed to cover different directions at the spatial frequency domain. This combination of angular- and time- multiplexing in addition with holographic recording allows the complex amplitude recovery of a set of elementary apertures covering different portions of the object's spectrum. Finally, the expanded synthetic aperture (SA) is generated by coherent addition of the set of recovered elementary apertures. Such SA expands up the cut-off frequency limit of the imaging system and allows getting a superresolved image of the input object. Moreover, if a priori knowledge about the input object is available, customized SA shaping is possible by considering the addition of those elementary apertures corresponding with only the directions of interest and, thus, reducing the whole consuming time of the approach. We present experimental results in concordance with theoretical predictions for two different resolution test objects, for different SA shapes, and considering different resolution gain factors.

  2. RGB digital lensless holographic microscopy

    NASA Astrophysics Data System (ADS)

    Garcia-Sucerquia, Jorge

    2013-11-01

    The recent introduction of color digital lensless holographic microscopy (CDLHM) has shown the possibility of imaging microscopic specimens at full color without the need of lenses. Owing to the simplicity, robustness, and compactness of the digital lensless holographic microscopes (DLHM), they have been presented as the ideal candidates to being developed into portable holographic microscopes. However, in the case of CDLHM the utilization of three independent lasers hinders the portability option for this microscope. In this contribution an alternative to reduce the complexity of CDLHM aimed to recover the portability of this microscopy technology is presented. A super-bright white-light light-emitting diode (LED) is spectrally and spatially filtered to produce the needed illumination by CDLHM to work. CDLHM with LED illumination is used to image at full color a section of the head of a drosophila melanogaster fly (fruit fly). The LED-CDLHM method shows the capability of imaging objects of 2μm size in comparison with the micrometer resolution reported for LASER-CDLHM.

  3. Quantitative phase imaging by wide field lensless digital holographic microscope

    NASA Astrophysics Data System (ADS)

    Adinda-Ougba, A.; Koukourakis, N.; Essaidi, A.; Ger­hardt, N. C.; Hofmann, M. R.

    2015-05-01

    Wide field, lensless microscopes have been developed for telemedicine and for resource limited setting [1]. They are based on in-line digital holography which is capable to provide amplitude and phase information resulting from numerical reconstruction. The phase information enables achieving axial resolution in the nanometer range. Hence, such microscopes provide a powerful tool to determine three-dimensional topologies of microstructures. In this contribution, a compact, low-cost, wide field, lensless microscope is presented, which is capable of providing topological profiles of microstructures in transparent material. Our setup consist only of two main components: a CMOSsensor chip and a laser diode without any need of a pinhole. We use this very simple setup to record holograms of microobjects. A wide field of view of ~24 mm², and a lateral resolution of ~2 μm are achieved. Moreover, amplitude and phase information are obtained from the numerical reconstruction of the holograms using a phase retrieval algorithm together with the angular spectrum propagation method. Topographic information of highly transparent micro-objects is obtained from the phase data. We evaluate our system by recording holograms of lines with different depths written by a focused laser beam. A reliable characterization of laser written microstructures is crucial for their functionality. Our results show that this system is valuable for determination of topological profiles of microstructures in transparent material.

  4. Photon counting digital holography

    NASA Astrophysics Data System (ADS)

    Demoli, Nazif; Skenderović, Hrvoje; Stipčević, Mario; Pavičić, Mladen

    2016-05-01

    Digital holography uses electronic sensors for hologram recording and numerical method for hologram reconstruction enabling thus the development of advanced holography applications. However, in some cases, the useful information is concealed in a very wide dynamic range of illumination intensities and successful recording requires an appropriate dynamic range of the sensor. An effective solution to this problem is the use of a photon-counting detector. Such detectors possess counting rates of the order of tens to hundreds of millions counts per second, but conditions of recording holograms have to be investigated in greater detail. Here, we summarize our main findings on this problem. First, conditions for optimum recording of digital holograms for detecting a signal significantly below detector's noise are analyzed in terms of the most important holographic measures. Second, for time-averaged digital holograms, optimum recordings were investigated for exposures shorter than the vibration cycle. In both cases, these conditions are studied by simulations and experiments.

  5. Comparative digital holography.

    PubMed

    Osten, Wolfgang; Baumbach, Torsten; Jüptner, Werner

    2002-01-01

    We described a method for direct holographic comparison of the shape or the deformation of two objects when it is not necessary that both samples be located at the same place. In contrast to the well-known incoherent techniques based on inverse fringe projection, this new approach uses a coherent mask that is imaged onto a sample object that has a microstructure different from that of the master object. The coherent mask is created by digital holography to permit instant access to complete optical information on the master object at any wanted place. Transmission of the digital master holograms to the relevant places can be made with a broadband digital telecommunication network such as the Internet.

  6. Generalized phase-shifting color digital holography

    NASA Astrophysics Data System (ADS)

    Nomura, Takanori; Kawakami, Takaaki; Shinomura, Kazuma

    2016-06-01

    Two methods to apply the generalized phase-shifting digital holography to color digital holography are proposed. One is wave-splitting generalized phase-shifting color digital holography. This is realized by using a color Bayer camera. Another is multiple exposure generalized phase-shifting color digital holography. This is realized by the wavelength-dependent phase-shifting devices. Experimental results for both generalized phase-shifting color digital holography are presented to confirm the proposed methods.

  7. Measurement of hygroscopic strain in deodar wood during convective drying using lensless Fourier transform digial holography

    NASA Astrophysics Data System (ADS)

    Kumar, Manoj; Shakher, Chandra

    2016-04-01

    In this paper, moisture induced deformation and shrinkage behaviour of deodar wood during convective drying is experimentally investigated by using digital holographic interferometry. There induces dimensional changes in wood due to the moisture absorption and desorption. Lensless Fourier transform digital holographic interferometry (LLFTDH) is used to study the moisture induced deformation and strain distribution in deodar wood. The proposed technique having high sensitivity and enables the observation of deformation and strain distribution during the variations of moisture content in the deodar wood.

  8. Lensless coherent imaging by sampling of the optical field with digital micromirror device

    NASA Astrophysics Data System (ADS)

    Vdovin, G.; Gong, H.; Soloviev, O.; Pozzi, P.; Verhaegen, M.

    2015-12-01

    We have experimentally demonstrated a lensless coherent microscope based on direct registration of the complex optical field by sampling the pupil with a sequence of two-point interferometers formed by a digital micromirror device. Complete registration of the complex amplitude in the pupil of the imaging system, without any reference beam, provides a convenient link between the experimental and computational optics. Unlike other approaches to digital holography, our method does not require any external reference beam, resulting in a simple and robust registration setup. Computer analysis of the experimentally registered field allows for focusing the image in the whole range from zero to infinity, and for virtual correction of the aberrations present in the real optical system, by applying the adaptive wavefront corrections to its virtual model.

  9. Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization.

    PubMed

    Bourgade, Thomas; Jianfei, Sun; Wang, Zhaomin; Elsa, Rosmin; Asundi, Anand

    2016-01-01

    A micro-electro-mechanical-system (MEMS) is a widely used component in many industries, including energy, biotechnology, medical, communications, and automotive. However, effective inspection and characterization metrology systems are needed to ensure the functional reliability of MEMS. This study presents a system based on digital holography as a tool for MEMS metrology. Digital holography has gained increasing attention in the past 20 years. With the fast development and decreasing cost of sensor arrays, resolution of such systems has increased broadening potential applications. Thus, it has attracted attention from both research and industry sides as a potential reliable tool for industrial metrology. Indeed, by recording the interference pattern between an object beam (which contains sample height information) and a reference beam on a CCD camera, one can retrieve the quantitative phase information of an object. However, most of digital holographic systems are bulky and thus not easy to implement on industry production lines. The novelty of the system presented is that it is lens-less and thus very compact. In this study, it is shown that the Compact Digital Holographic Microscope (CDHM) can be used to evaluate several characteristics typically consider as criteria in MEMS inspections. The surface profiles of MEMS in both static and dynamic conditions are presented. Comparison with AFM is investigated to validate the accuracy of the CDHM.

  10. Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization.

    PubMed

    Bourgade, Thomas; Jianfei, Sun; Wang, Zhaomin; Elsa, Rosmin; Asundi, Anand

    2016-01-01

    A micro-electro-mechanical-system (MEMS) is a widely used component in many industries, including energy, biotechnology, medical, communications, and automotive. However, effective inspection and characterization metrology systems are needed to ensure the functional reliability of MEMS. This study presents a system based on digital holography as a tool for MEMS metrology. Digital holography has gained increasing attention in the past 20 years. With the fast development and decreasing cost of sensor arrays, resolution of such systems has increased broadening potential applications. Thus, it has attracted attention from both research and industry sides as a potential reliable tool for industrial metrology. Indeed, by recording the interference pattern between an object beam (which contains sample height information) and a reference beam on a CCD camera, one can retrieve the quantitative phase information of an object. However, most of digital holographic systems are bulky and thus not easy to implement on industry production lines. The novelty of the system presented is that it is lens-less and thus very compact. In this study, it is shown that the Compact Digital Holographic Microscope (CDHM) can be used to evaluate several characteristics typically consider as criteria in MEMS inspections. The surface profiles of MEMS in both static and dynamic conditions are presented. Comparison with AFM is investigated to validate the accuracy of the CDHM. PMID:27404277

  11. Color digital lensless holographic microscopy: laser versus LED illumination.

    PubMed

    Garcia-Sucerquia, Jorge

    2016-08-20

    A comparison of the performance of color digital lensless holographic microscopy (CDLHM) as utilized for illumination of RGB lasers or a super-bright white-light LED with a set of spectral filters is presented. As the use of lasers in CDLHM conceals the possibility of having a compact, lightweight, portable, and low cost microscope, and additionally the limited available laser radiation wavelengths limit a real multispectral imaging microscope, here we present the use of super-bright white-light LED and spectral filters for illuminating the sample. The performance of RGB laser-CDLHM and LED-CDLHM is evaluated on imaging a section of the head of a Drosophila melanogaster fly. This comparison shows that there is trade-off between the spatial resolution of the microscope and the light sources utilized, which can be understood with regard to the coherence properties of the illuminating light. Despite the smaller spatial coherence features of LED-CDLHM in comparison with laser-CDLHM, the former shows promise as a portable RGB digital lensless holographic microscope that could be extended to other wavelengths by the use of different spectral filters. PMID:27556985

  12. Twin image removal in digital in-line holography based on iterative inter-projections

    NASA Astrophysics Data System (ADS)

    Chen, Bing Kuan; Chen, Tai-Yu; Hung, Shau Gang; Huang, Sheng-Lung; Lin, Jiunn-Yuan

    2016-06-01

    A simple and efficient phase retrieval method based on the iterative inter-projections of the recorded Fourier modulus between two effective holographic planes is developed to eliminate the twin image in digital in-line holography. The proposed algorithm converges stably in phase extraction procedures without requiring any prior knowledge or sophisticated support of the object and is applicable to lensless Gabor and Fourier holography as well as holographic microscopy with imaging lenses. Numerical and experimental results suggest that the spatial resolution enhancement on the reconstructed image can be achieved with this technique due to the capability of recovering the diffraction phases of low-intensity signals.

  13. Imaging live humans through smoke and flames using far-infrared digital holography.

    PubMed

    Locatelli, M; Pugliese, E; Paturzo, M; Bianco, V; Finizio, A; Pelagotti, A; Poggi, P; Miccio, L; Meucci, R; Ferraro, P

    2013-03-11

    The ability to see behind flames is a key challenge for the industrial field and particularly for the safety field. Development of new technologies to detect live people through smoke and flames in fire scenes is an extremely desirable goal since it can save human lives. The latest technologies, including equipment adopted by fire departments, use infrared bolometers for infrared digital cameras that allow users to see through smoke. However, such detectors are blinded by flame-emitted radiation. Here we show a completely different approach that makes use of lensless digital holography technology in the infrared range for successful imaging through smoke and flames. Notably, we demonstrate that digital holography with a cw laser allows the recording of dynamic human-size targets. In this work, easy detection of live, moving people is achieved through both smoke and flames, thus demonstrating the capability of digital holography at 10.6 μm. PMID:23482109

  14. Lensless Imaging and Sensing.

    PubMed

    Ozcan, Aydogan; McLeod, Euan

    2016-07-11

    High-resolution optical microscopy has traditionally relied on high-magnification and high-numerical aperture objective lenses. In contrast, lensless microscopy can provide high-resolution images without the use of any focusing lenses, offering the advantages of a large field of view, high resolution, cost-effectiveness, portability, and depth-resolved three-dimensional (3D) imaging. Here we review various approaches to lensless imaging, as well as its applications in biosensing, diagnostics, and cytometry. These approaches include shadow imaging, fluorescence, holography, superresolution 3D imaging, iterative phase recovery, and color imaging. These approaches share a reliance on computational techniques, which are typically necessary to reconstruct meaningful images from the raw data captured by digital image sensors. When these approaches are combined with physical innovations in sample preparation and fabrication, lensless imaging can be used to image and sense cells, viruses, nanoparticles, and biomolecules. We conclude by discussing several ways in which lensless imaging and sensing might develop in the near future. PMID:27420569

  15. Lensless Imaging and Sensing.

    PubMed

    Ozcan, Aydogan; McLeod, Euan

    2016-07-11

    High-resolution optical microscopy has traditionally relied on high-magnification and high-numerical aperture objective lenses. In contrast, lensless microscopy can provide high-resolution images without the use of any focusing lenses, offering the advantages of a large field of view, high resolution, cost-effectiveness, portability, and depth-resolved three-dimensional (3D) imaging. Here we review various approaches to lensless imaging, as well as its applications in biosensing, diagnostics, and cytometry. These approaches include shadow imaging, fluorescence, holography, superresolution 3D imaging, iterative phase recovery, and color imaging. These approaches share a reliance on computational techniques, which are typically necessary to reconstruct meaningful images from the raw data captured by digital image sensors. When these approaches are combined with physical innovations in sample preparation and fabrication, lensless imaging can be used to image and sense cells, viruses, nanoparticles, and biomolecules. We conclude by discussing several ways in which lensless imaging and sensing might develop in the near future.

  16. Direct to Digital Holography

    SciTech Connect

    Bingham, P.R.; Tobin, K.W.

    2007-09-30

    In this Cooperative Research and Development Agreement (CRADA), Oak Ridge National Laboratory (ORNL) assisted nLine Corporation of Austin, TX in the development of prototype semiconductor wafer inspection tools based on the direct-to-digital holographic (DDH) techniques invented at ORNL. Key components of this work included, testing of DDH for detection of defects in High Aspect Ratio (HAR) structures, development of image processing techniques to enhance detection capabilities through the use of both phase and intensity, and development of methods for autofocus on the DDH tools.

  17. Label-free coherent microscopy through blood by digital holography

    NASA Astrophysics Data System (ADS)

    Bianco, V.; Paturzo, M.; Merola, F.; Miccio, L.; Memmolo, P.; Gennari, O.; Ferraro, P.

    2015-05-01

    Digital Holography (DH) numerical methods have been developed to allow imaging through turbid media. DH is a wide used imaging technique as it provides non-invasive quantitative phase-contrast mapping as well as flexible numerical refocusing of samples acquired in lens-based or lensless conditions. However, a challenging issue has to be faced when the samples are immersed inside a dynamic turbid medium, as biological occluding objects out of interest provoke severe light scattering or unpredictable time-variable phase delays which scramble the object information, so that in many cases the sample is not visible at all. Here we show a simple technique, named Multi-Look Digital Holography (MLDH), able to fully recover the useful signal of the specimen of interest dipped inside the turbid liquid phase. Multiple hologram recordings are incoherently combined to synthesize the whole complex field carrying the useful information, thus revealing the hidden objects. In particular, it will be shown that both amplitude imaging and phase-contrast mapping of cells hidden behind a flow of Red Blood Cells can be obtained. Besides, qualitative comparison and quantitative evaluation show a remarkable improvement with respect to the image captured when the cells were immersed in a transparent medium. In other words, the RBCs have been demonstrated to accomplish an optical task, acting as a speckle noise de-correlation device.

  18. Digital holography-based steganography.

    PubMed

    Hamam, Habib

    2010-12-15

    A steganographic method offering a high hiding capacity is presented in which the techniques of digital holography are used to distribute information from a small secret image across the larger pixel field of a cover image. An iterative algorithm is used to design a phase-only or complex hologram from a padded version of the secret image, quantizing this data according to the carrier data bits that are available within the intended cover image. By introducing the hologram data only into low-order bits of larger amplitude cover pixels, the change in the cover image remains imperceptible to the casual observer, with a peak signal-to-noise ratio of >40 dB.

  19. Correct self-assembling of spatial frequencies in super-resolution synthetic aperture digital holography.

    PubMed

    Paturzo, Melania; Ferraro, Pietro

    2009-12-01

    Synthetic aperture enlargement is obtained, in lensless digital holography, by introducing a diffraction grating between the object and the CCD camera with the aim of getting super-resolution. We demonstrate here that the spatial frequencies are naturally self-assembled in the reconstructed image plane when the NA is increased synthetically at its maximum extent of three times. By this approach it possible to avoid the use of the grating transmission formula in the numerical reconstruction process, thus reducing significantly the noise in the final super-resolved image. Demonstrations are reported in 1D and 2D with an optical target and a biological sample, respectively.

  20. Digital holography to light field

    NASA Astrophysics Data System (ADS)

    Asundi, Anand; Zuo, Chao

    2014-05-01

    Holography uses wave (physical) optical principles of interference and diffraction to record and display images. Interference allows us to record the amplitude and phase of the optical wave emanating from an object on a film or recording medium and diffraction enables us to see this wave-field, i.e. the amplitude and phase of the object. Visually this corresponds to both perspective and depth information being reconstructed as in the original scene. Digital Holography has enabled quantification of phase which in some applications provides meaningful engineering parameters. There is growing interest in reconstructing this wavefield without interference. Thus the non-interferometric Transport of Intensity Equation (TIE) method is gaining increased research, which uses two or more defocused images to reconstruct the phase. Due to its non-interferometric nature, TIE relaxes the stringent beam-coherence requirements for interferometry, extending its applications to various optical fields with arbitrary spatial and temporal coherence. The alternate school of thought emerges from the computer science community primarily deals with ray optics. In a normal imaging system all rays emerging from an object point into are focused to a conjugate image point. Information of ray direction is lost and thus the perspective and depth information. A light field image is one that has information of both amplitude and direction of rays fanning from any object point and thus provides perspective (or what could be termed as phase) of the object wave as well. It would thus be possible to extract phase as we know it from this albeit for a coherent illumination case.

  1. Synthesis aperture femtosecond-pulsed digital holography

    NASA Astrophysics Data System (ADS)

    Zhu, Linwei; Sun, Meiyu; Chen, Jiannong; Yu, Yongjiang; Zhou, Changhe

    2013-09-01

    A new aperture-synthesis approach in femtosecond-pulse digital holography for obtaining a high-resolution and a whole field of view of the reconstructed image is proposed. The subholograms are recorded only by delay scanning holograms that have different delay times between the object and reference beams. In addition, by using image processing techniques, the synthesis aperture digital hologram can be superposed accurately. Analysis and experimental results show that the walk-off in femtosecond off-axis digital holography caused by low coherent can be well eliminated. The resolution and the field of view of the reconstructed image can be improved effectively.

  2. GPU-based calculations in digital holography

    NASA Astrophysics Data System (ADS)

    Madrigal, R.; Acebal, P.; Blaya, S.; Carretero, L.; Fimia, A.; Serrano, F.

    2013-05-01

    In this work we are going to apply GPU (Graphical Processing Units) with CUDA environment for scientific calculations, concretely high cost computations on the field of digital holography. For this, we have studied three typical problems in digital holography such as Fourier transforms, Fresnel reconstruction of the hologram and the calculation of vectorial diffraction integral. In all cases the runtime at different image size and the corresponding accuracy were compared to the obtained by traditional calculation systems. The programs have been carried out on a computer with a graphic card of last generation, Nvidia GTX 680, which is optimized for integer calculations. As a result a large reduction of runtime has been obtained which allows a significant improvement. Concretely, 15 fold shorter times for Fresnel approximation calculations and 600 times for the vectorial diffraction integral. These initial results, open the possibility for applying such kind of calculations in real time digital holography.

  3. Multispectral digital lensless holographic microscopy: from femtosecond laser to white light LED

    NASA Astrophysics Data System (ADS)

    Garcia-Sucerquia, J.

    2015-04-01

    The use of femtosecond laser radiation and super bright white LED in digital lensless holographic microscopy is presented. For the ultrafast laser radiation two different configurations of operation of the microscope are presented and the dissimilar performance of each one analyzed. The microscope operating with a super bright white light LED in combination with optical filters shows very competitive performance as it is compared with more expensive optical sources. The broadband emission of both radiation sources allows the multispectral imaging of biological samples to obtain spectral responses and/or full color images of the microscopic specimens; sections of the head of a Drosophila melanogaster fly are imaged in this contribution. The simple, solid, compact, lightweight, and reliable architecture of digital lensless holographic microscopy operating with broadband light sources to image biological specimens exhibiting micrometer-sized details is evaluated in the present contribution.

  4. Subaperture stitching interferometry based on digital holography

    NASA Astrophysics Data System (ADS)

    Pan, Feng; Lu, Xiaoyun; Dong, Bin; Ma, Xichao; Xiao, Wen

    2016-11-01

    A novel subaperture stitching interferometry based on digital holography is developed to measure the deformation of spherical surfaces. The subaperture measurement is performed by off-axis digital holography on single exposure. Then, the subaperture phase maps are obtained by digital holographic reconstruction, in which the phase aberration caused by position errors of each subaperture measurement is effectively compensated by the method of numerical parametric lens. After that, the full aperture phase map is retrieved by a subaperture stitching algorithm, in which the relative alignment errors of adjacent subapertures are eliminated with an iterative process of stitching optimization. The experiments demonstrate the feasibility and effectiveness of the proposed interferometry, which provides a rapid and robust way to measure spherical surfaces with high resolution and precision. A practical example is given to demonstrate the performance of this method. The stitching result shows good agreement with the full-aperture result.

  5. Super-resolution imaging in digital holography by using dynamic grating with a spatial light modulator

    NASA Astrophysics Data System (ADS)

    Lin, Qiaowen; Wang, Dayong; Wang, Yunxin; Rong, Lu; Chang, Shifeng

    2015-03-01

    A super-resolution imaging method using dynamic grating based on liquid-crystal spatial light modulator (SLM) is developed to improve the resolution of a digital holographic system. The one-dimensional amplitude cosine grating is loaded on the SLM, which is placed between the object and hologram plane in order to collect more high-frequency components towards CCD plane. The point spread function of the system is given to confirm the separation condition of reconstructed images for multiple diffraction orders. The simulation and experiments are carried out for a standard resolution test target as a sample, which confirms that the imaging resolution is improved from 55.7 μm to 31.3 μm compared with traditional lensless Fourier transform digital holography. The unique advantage of the proposed method is that the period of the grating can be programmably adjusted according to the separation condition.

  6. Seeing through smoke and flames: a challenge for imaging capabilities, met thanks to digital holography at far infrared

    NASA Astrophysics Data System (ADS)

    Locatelli, Massimiliano; Pugliese, Eugenio; Paturzo, Melania; Bianco, Vittorio; Finizio, Andrea; Pelagotti, Anna; Poggi, Pasquale; Miccio, Lisa; Meucci, Riccardo; Ferraro, Pietro

    2013-04-01

    We show that imaging alive people through smoke and flames is possible by Digital Holography at far infrared. This capability is of crucial importance in the security field to provide a new tool for firefighters and first responders in fire accidents. So far, the existing thermographic infrared cameras allows to see people through dense smoke, sensing the radiation emitted by human body. However, these devices are often blinded due to the flame emission, which is collected by the zoom lenses employed for the scope, and the information of the targets beyond the flames is unavoidably lost. On the contrary, lensless Digital Holography at far infrared avoids the typical saturation of the camera detectors returning clear images of targets seen behind veils of smoke and curtains of flames. Moreover, we demonstrate that human-size holograms can be recorded, allowing to move this promising technology outside the lab for safety applications.

  7. Lensless two-photon imaging through a multicore fiber with coherence-gated digital phase conjugation.

    PubMed

    Conkey, Donald B; Stasio, Nicolino; Morales-Delgado, Edgar E; Romito, Marilisa; Moser, Christophe; Psaltis, Demetri

    2016-04-30

    We performed near-diffraction limited two-photon fluorescence (TPF) imaging through a lensless, multicore-fiber (MCF) endoscope utilizing digital phase conjugation. The phase conjugation technique is compatible with commercially available MCFs with high core density. We demonstrate focusing of ultrashort pulses through an MCF and show that the method allows for resolution that is not limited by the MCF core spacing. We constructed TPF images of fluorescent beads and cells by digital scanning of the phase-conjugated focus on the target object and collection of the emitted fluorescence through the MCF.

  8. Vertical-bandwidth-limited digital holography.

    PubMed

    Liu, Jung-Ping; Lee, Chieh-Cheng; Lo, Ying-Hau; Luo, Dao-Zheng

    2012-07-01

    Optical scanning holography (OSH) is a promising technique to acquire a big-size digital hologram. However, the acquisition speed is limited by the mechanical scanner. In this Letter we apply the OSH in conjunction with an anisotropic low-pass filtering pupil to acquire vertical-bandwidth-limited (VBL) holograms. The size and the acquisition time of the VBL hologram can be reduced by one order of magnitude while the horizontal resolution remains the same as the conventional scanning hologram. The VBL hologram can be coded as an off-axis hologram without any postfiltering. Meanwhile, the full horizontal bandwidth of the displaying device can be capitalized.

  9. EDITORIAL: Optical tomography and digital holography

    NASA Astrophysics Data System (ADS)

    Coupland, Jeremy; Lobera, Julia

    2008-07-01

    The articles in this special feature in Measurement Science and Technology concern exciting new developments in the field of digital holography—the process of electronically recording and numerically reconstructing an optical field [1]. Making use of the enormous advances in digital imaging and computer technology, digital holography is presented in a range of applications from fluid flow measurement and structural analysis to medical imaging. The science of digital holography rests on the foundations of optical holography, on the work of Gabor in the late 1940s, and on the development of laser sources in the 1960s, which made his vision a practical reality [2]. Optical holography, however, uses a photosensitive material, both to record a latent image and subsequently to behave as a diffractive optical element with which to reconstruct the incident field. In this way display holograms, using silver halide materials for example, can produce life-size images that are virtually indistinguishable from the object itself [3]. Digital holography, in contrast, separates the steps of recording and reconstruction, and the final image is most often in the form of a 3D computer model. Of course, television cameras have been used from the beginnings of holography to record interferometric images. However, the huge disparity between the resolution of holographic recording materials (more than 3000 cycles/mm) and television cameras (around 50 cycles/mm) was raised as a major concern by early researchers. TV holography, as it was sometimes called, generally recorded low numerical aperture (NA) holograms producing images with characteristically large speckle and was therefore more often referred to as electronic speckle pattern interferomery (ESPI) [4]. It is possible, however, to record large NA holograms on a sensor with restricted resolution by using an objective lens or a diverging reference wave [5]. This is generally referred to as digital holographic microscopy (DHM) since

  10. Direct-to-digital holography and holovision

    DOEpatents

    Thomas, Clarence E.; Baylor, Larry R.; Hanson, Gregory R.; Rasmussen, David A.; Voelkl, Edgar; Castracane, James; Simkulet, Michelle; Clow, Lawrence

    2000-01-01

    Systems and methods for direct-to-digital holography are described. An apparatus includes a laser; a beamsplitter optically coupled to the laser; a reference beam mirror optically coupled to the beamsplitter; an object optically coupled to the beamsplitter, a focusing lens optically coupled to both the reference beam mirror and the object; and a digital recorder optically coupled to the focusing lens. A reference beam is incident upon the reference beam mirror at a non-normal angle, and the reference beam and an object beam are focused by the focusing lens at a focal plane of the digital recorder to form an image. The systems and methods provide advantages in that computer assisted holographic measurements can be made.

  11. Lensless multispectral digital in-line holographic microscope

    NASA Astrophysics Data System (ADS)

    Ryle, James P.; McDonnell, Susan; Sheridan, John T.

    2011-12-01

    An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.

  12. Remote metrology by comparative digital holography

    SciTech Connect

    Baumbach, Torsten; Osten, Wolfgang; Kopylow, Christoph von; Jueptner, Werner

    2006-02-10

    A method for the remote comparison of objects with regard to their shape or response to a load is presented. The method allows interferometric sensitivity for comparing objects with different microstructure. In contrast to the well-known incoherent techniques based on inverse fringe projection this new approach uses the coherent optical wave field of the master object as a mask for the illumination of the sample object. The coherent mask is created by digital holography to allow instant access to the complete optical information of the master object at any place desired. The mask is reconstructed by a spatial light modulator (SLM). The optical reconstruction of digital holograms with SLM technology allows modification of reconstructed wavefronts with respect to improvement of image quality, the skilled introduction of additional information about the object (augmented reality), and the alignment of the master and test object.

  13. Wavelet image processing applied to optical and digital holography: past achievements and future challenges

    NASA Astrophysics Data System (ADS)

    Jones, Katharine J.

    2005-08-01

    The link between wavelets and optics goes back to the work of Dennis Gabor who both invented holography and developed Gabor decompositions. Holography involves 3-D images. Gabor decompositions involves 1-D signals. Gabor decompositions are the predecessors of wavelets. Wavelet image processing of holography, both optical holography and digital holography, will be examined with respect to past achievements and future challenges.

  14. Multispectral lensless digital holographic microscope: imaging MCF-7 and MDA-MB-231 cancer cell cultures

    NASA Astrophysics Data System (ADS)

    Ryle, James P.; Molony, Karen M.; McDonnell, Susan; Naughton, Thomas J.; Sheridan, John T.

    2009-08-01

    Digital holography is the process where an object's phase and amplitude information is retrieved from intensity images obtained using a digital camera (e.g. CCD or CMOS sensor). In-line digital holographic techniques offer full use of the recording device's sampling bandwidth, unlike off-axis holography where object information is not modulated onto carrier fringes. Reconstructed images are obscured by the linear superposition of the unwanted, out of focus, twin images. In addition to this, speckle noise degrades overall quality of the reconstructed images. The speckle effect is a phenomenon of laser sources used in digital holographic systems. Minimizing the effects due to speckle noise, removal of the twin image and using the full sampling bandwidth of the capture device aids overall reconstructed image quality. Such improvements applied to digital holography can benefit applications such as holographic microscopy where the reconstructed images are obscured with twin image information. Overcoming such problems allows greater flexibility in current image processing techniques, which can be applied to segmenting biological cells (e.g. MCF-7 and MDA-MB- 231) to determine their overall cell density and viability. This could potentially be used to distinguish between apoptotic and necrotic cells in large scale mammalian cell processes, currently the system of choice, within the biopharmaceutical industry.

  15. Second-harmonic illumination to enhance multispectral digital lensless holographic microscopy.

    PubMed

    Mendoza-Yero, Omel; Carbonell-Leal, Miguel; Lancis, Jesús; Garcia-Sucerquia, Jorge

    2016-03-01

    Multispectral digital lensless holographic microscopy (MDLHM) operating with second-harmonic illumination is shown. Added to the improvement of the spatial resolution of the previously reported MDLHM operating with near-infrared illumination, this second-harmonic MDLHM shows promise as a tool to study the behavior of biological samples under a broad spectral illumination. This illumination is generated by focusing a highly spatially coherent ultrashort pulsed radiation into an uncoated Type 1 β-BaB2O4 (BBO) nonlinear crystal. The second-harmonic MDLHM allows achieving multispectral images of biological samples with enhanced micrometer spatial resolution. The illumination wavelength of the second-harmonic MDLHM can be tuned by displacing a focusing optics with respect to a pinhole; spatially resolved information at different wavelengths of the sample can then be retrieved.

  16. Dual-mode lensless imaging device for digital enzyme linked immunosorbent assay

    NASA Astrophysics Data System (ADS)

    Sasagawa, Kiyotaka; Kim, Soo Heyon; Miyazawa, Kazuya; Takehara, Hironari; Noda, Toshihiko; Tokuda, Takashi; Iino, Ryota; Noji, Hiroyuki; Ohta, Jun

    2014-03-01

    Digital enzyme linked immunosorbent assay (ELISA) is an ultra-sensitive technology for detecting biomarkers and viruses etc. As a conventional ELISA technique, a target molecule is bonded to an antibody with an enzyme by antigen-antibody reaction. In this technology, a femto-liter droplet chamber array is used as reaction chambers. Due to its small volume, the concentration of fluorescent product by single enzyme can be sufficient for detection by a fluorescent microscopy. In this work, we demonstrate a miniaturized lensless imaging device for digital ELISA by using a custom image sensor. The pixel array of the sensor is coated with a 20 μm-thick yellow filter to eliminate excitation light at 470 nm and covered by a fiber optic plate (FOP) to protect the sensor without resolution degradation. The droplet chamber array formed on a 50μm-thick glass plate is directly placed on the FOP. In the digital ELISA, microbeads coated with antibody are loaded into the droplet chamber array, and the ratio of the fluorescent to the non-fluorescent chambers with the microbeads are observed. In the fluorescence imaging, the spatial resolution is degraded by the spreading through the glass plate because the fluorescence is irradiated omnidirectionally. This degradation is compensated by image processing and the resolution of ~35 μm was achieved. In the bright field imaging, the projected images of the beads with collimated illumination are observed. By varying the incident angle and image composition, microbeads were successfully imaged.

  17. Single-exposure two-dimensional superresolution in digital holography using a vertical cavity surface-emitting laser source array.

    PubMed

    Granero, Luis; Zalevsky, Zeev; Micó, Vicente

    2011-04-01

    We present a new implementation capable of producing two-dimensional (2D) superresolution (SR) imaging in a single exposure by aperture synthesis in digital lensless Fourier holography when using angular multiplexing provided by a vertical cavity surface-emitting laser source array. The system performs the recording in a single CCD snapshot of a multiplexed hologram coming from the incoherent addition of multiple subholograms, where each contains information about a different 2D spatial frequency band of the object's spectrum. Thus, a set of nonoverlapping bandpass images of the input object can be recovered by Fourier transformation (FT) of the multiplexed hologram. The SR is obtained by coherent addition of the information contained in each bandpass image while generating an enlarged synthetic aperture. Experimental results demonstrate improvement in resolution and image quality.

  18. Single-exposure two-dimensional superresolution in digital holography using a vertical cavity surface-emitting laser source array.

    PubMed

    Granero, Luis; Zalevsky, Zeev; Micó, Vicente

    2011-04-01

    We present a new implementation capable of producing two-dimensional (2D) superresolution (SR) imaging in a single exposure by aperture synthesis in digital lensless Fourier holography when using angular multiplexing provided by a vertical cavity surface-emitting laser source array. The system performs the recording in a single CCD snapshot of a multiplexed hologram coming from the incoherent addition of multiple subholograms, where each contains information about a different 2D spatial frequency band of the object's spectrum. Thus, a set of nonoverlapping bandpass images of the input object can be recovered by Fourier transformation (FT) of the multiplexed hologram. The SR is obtained by coherent addition of the information contained in each bandpass image while generating an enlarged synthetic aperture. Experimental results demonstrate improvement in resolution and image quality. PMID:21479012

  19. High temperature measurements of martensitic transformations using digital holography.

    PubMed

    Thiesing, Benjamin P; Mann, Christopher J; Dryepondt, Sebastien

    2013-07-01

    During thermal cycling of nickel-aluminum-platinum (NiAlPt) and single crystal iron-chromium-nickel (FeCrNi) alloys, the structural changes associated with the martensite to austenite phase transformation were measured using dual-wavelength digital holography. Real-time in situ measurements reveal the formation of striations within the NiAlPt alloy at 70°C and the FeCrNi alloy at 520°C. The results demonstrate that digital holography is an effective technique for acquiring noncontact, high precision information of the surface evolution of alloys at high temperatures.

  20. High Temperature Measurements Of Martensitic transformations Using Digital Holography

    SciTech Connect

    Thiesing, Benjamin; Mann, Christopher J; Dryepondt, Sebastien N

    2013-01-01

    During thermal cycling of nickel-aluminum-platinum (NiAlPt) and single crystal Fe-15Cr-15Ni alloys, the structural changes associated with the martensite to austenite phase transformation were measured using dual-wavelength digital holography. Real-time in-situ measurements reveal the formation of striations within the NiPtAl alloy at 70 C and the FeCrNi alloy at 520 C. The results demonstrate that digital holography is an effective technique for acquiring non-contact, high precision information of the surface evolution of alloys at high temperatures.

  1. High temperature measurements of martensitic transformations using digital holography.

    PubMed

    Thiesing, Benjamin P; Mann, Christopher J; Dryepondt, Sebastien

    2013-07-01

    During thermal cycling of nickel-aluminum-platinum (NiAlPt) and single crystal iron-chromium-nickel (FeCrNi) alloys, the structural changes associated with the martensite to austenite phase transformation were measured using dual-wavelength digital holography. Real-time in situ measurements reveal the formation of striations within the NiAlPt alloy at 70°C and the FeCrNi alloy at 520°C. The results demonstrate that digital holography is an effective technique for acquiring noncontact, high precision information of the surface evolution of alloys at high temperatures. PMID:23842235

  2. Parallel phase-shifting digital holography using LCOS-SLM

    NASA Astrophysics Data System (ADS)

    Lin, Miao; Nitta, Kouichi; Matoba, Osamu; Awatsuji, Yasuhiro

    2013-12-01

    Parallel phase-shifting digital holography (PPSDH) method can record dynamic three-dimensional events with higher spatial resolution than that of off-axis digital holography. In PPSDH, all amount of phase retardations are included in a multiplexed hologram and can be optically implemented by using a phase-mode spatial light modulator (SLM) located in the reference beam. The SLM can also compensate optical aberration caused by lenses, beam splitters, and air fluctuation as in adaptive optics. In this presentation, we review our experimental results using stationary two-dimensional object using a liquid-crystal on Silicon (LCOS) SLM.

  3. Terahertz digital holography image processing based on MAP algorithm

    NASA Astrophysics Data System (ADS)

    Chen, Guang-Hao; Li, Qi

    2015-04-01

    Terahertz digital holography combines the terahertz technology and digital holography technology at present, fully exploits the advantages in both of them. Unfortunately, the quality of terahertz digital holography reconstruction images is gravely harmed by speckle noise which hinders the popularization of this technology. In this paper, the maximum a posterior estimation (MAP) filter is harnessed for the restoration of the digital reconstruction images. The filtering results are compared with images filtered by Wiener Filter and conventional frequency-domain filters from both subjective and objective perspectives. As for objective assessment, we adopted speckle index (SPKI) and edge preserving index (EPI) to quantitate the quality of images. In this paper, Canny edge detector is also used to outline the target in original and reconstruction images, which then act as an important role in the evaluation of filter performance. All the analysis indicate that maximum a posterior estimation filtering algorithm performs superiorly compared with the other two competitors in this paper and has enhanced the terahertz digital holography reconstruction images to a certain degree, allowing for a more accurate boundary identification.

  4. Superresolved digital in-line holographic microscopy for high-resolution lensless biological imaging

    NASA Astrophysics Data System (ADS)

    Micó, Vicente; Zalevsky, Zeev

    2010-07-01

    Digital in-line holographic microscopy (DIHM) is a modern approach capable of achieving micron-range lateral and depth resolutions in three-dimensional imaging. DIHM in combination with numerical imaging reconstruction uses an extremely simplified setup while retaining the advantages provided by holography with enhanced capabilities derived from algorithmic digital processing. We introduce superresolved DIHM incoming from time and angular multiplexing of the sample spatial frequency information and yielding in the generation of a synthetic aperture (SA). The SA expands the cutoff frequency of the imaging system, allowing submicron resolutions in both transversal and axial directions. The proposed approach can be applied when imaging essentially transparent (low-concentration dilutions) and static (slow dynamics) samples. Validation of the method for both a synthetic object (U.S. Air Force resolution test) to quantify the resolution improvement and a biological specimen (sperm cells biosample) are reported showing the generation of high synthetic numerical aperture values working without lenses.

  5. A digital holography set-up for 3D vortex flow dynamics

    NASA Astrophysics Data System (ADS)

    Lebon, Benoît; Perret, Gaële; Coëtmellec, Sébastien; Godard, Gilles; Gréhan, Gérard; Lebrun, Denis; Brossard, Jérôme

    2016-06-01

    In the present paper, a digital in-line holography (DIH) set-up, with a converging beam, is used to take three-dimensional (3D) velocity measurements of vortices. The vortices are formed periodically at the edges of a submerged horizontal plate submitted to regular waves. They take the form of vortex filaments that extend from side to side of the channel. They undergo strongly three-dimensional instability mechanisms that remain very complicated to characterize experimentally. The experiments are performed in a 10 × 0.3 × 0.3 m3 wave flume. The DIH set-up is performed using a modulated laser diode emitting at the wavelength of 640 nm and a lensless CCD camera. The beam crosses the channel side to side. To reveal the flow dynamics, 30-μm hydrogen bubbles are generated at the edge of the plate to serve as tracers. Their locations are recorded on the holograms multiple times to access the dynamics of the flow. This method leads to an accuracy in the order of 100 μm on the axial location. Those measurements have been validated with stereo-PIV measurements. A very good agreement is found on time-averaged velocity fields between the two techniques.

  6. Resolution enhancement phase-contrast imaging by microsphere digital holography

    NASA Astrophysics Data System (ADS)

    Wang, Yunxin; Guo, Sha; Wang, Dayong; Lin, Qiaowen; Rong, Lu; Zhao, Jie

    2016-05-01

    Microsphere has shown the superiority of super-resolution imaging in the traditional 2D intensity microscope. Here a microsphere digital holography approach is presented to realize the resolution enhancement phase-contrast imaging. The system is designed by combining the microsphere with the image-plane digital holography. A microsphere very close to the object can increase the resolution by transforming the object wave from the higher frequency to the lower one. The resolution enhancement amplitude and phase images can be retrieved from a single hologram. The experiments are carried on the 1D and 2D gratings, and the results demonstrate that the observed resolution has been improved, meanwhile, the phase-contrast image is obtained. The proposed method can improve the transverse resolution in all directions based on a single exposure. Furthermore, this system has extended the application of the microsphere from the conventional 2D microscopic imaging to 3D phase-contrast microscopic imaging.

  7. Dynamic modal characterization of musical instruments using digital holography.

    PubMed

    Demoli, Nazif; Demoli, Ivan

    2005-06-27

    This study shows that a dynamic modal characterization of musical instruments with membrane can be carried out using a low-cost device and that the obtained very informative results can be presented as a movie. The proposed device is based on a digital holography technique using the quasi-Fourier configuration and time-average principle. Its practical realization with a commercial digital camera and large plane mirrors allows relatively simple analyzing of big vibration surfaces. The experimental measurements given for a percussion instrument are supported by the mathematical formulation of the problem.

  8. Holography.

    ERIC Educational Resources Information Center

    Klein, H. Arthur

    Holography is a process which numbers among its many applications the creation of holograms--unique three dimensional photographs that show spatial relations and shifts just as they exist in reality. This book recounts the history of holography, tracing its development from Euclid's theory of light rays through Huygens' theory of wave motion to…

  9. Optical encryption in single shot digital holography

    NASA Astrophysics Data System (ADS)

    Lin, Ching-Yang; Chen, Gu-Liang; Kuo, Ming-Kuei; Chang, Chi-Ching; Yau, Hon-Fai

    2007-09-01

    We propose a novel optical encryption approach using a lenticular lens array (LLA) as a deterministic phase modulator and the single-shot digital holographic scheme. In the proposed scheme, the input amplitude image is encrypted and interferes with the reference wave phase, which is modulated by a LLA, then recorded holographically by a digital CCD camera to form an encrypted hologram. A decryption key is obtained from the key hologram using numerical reconstruction. The image is decrypted using a digital holographic approach after which the encrypted hologram is multiplying the numerical reconstructed key for decryption. The experimental results show that only an encrypted hologram is needed. Moreover with this approach, the decryption procedure can be rapidly accomplished using a personal computer, presenting a decrypted image of satisfactory image quality. Finally the selective sensitivity of the key rotation is also investigated.

  10. Full-field vibrometry with digital Fresnel holography

    SciTech Connect

    Leval, Julien; Picart, Pascal; Boileau, Jean Pierre; Pascal, Jean Claude

    2005-09-20

    A setup that permits full-field vibration amplitude and phase retrieval with digital Fresnel holography is presented. Full reconstruction of the vibration is achieved with a three-step stroboscopic holographic recording, and an extraction algorithm is proposed. The finite temporal width of the illuminating light is considered in an investigation of the distortion of the measured amplitude and phase. In particular, a theoretical analysis is proposed and compared with numerical simulations that show good agreement. Experimental results are presented for a loudspeaker under sinusoidal excitation; the mean quadratic velocity extracted from amplitude evaluation under two different measuring conditions is presented. Comparison with time averaging validates the full-field vibrometer.

  11. Dual wavelength digital holography for 3D particle image velocimetry

    NASA Astrophysics Data System (ADS)

    Grare, S.; Coëtmellec, S.,; Allano, D.; Grehan, G.; Brunel, M.; Lebrun, D.

    2015-02-01

    A multi-exposure digital in-line hologram of a moving particle field is recorded by two different wavelengths and at different times. As a result, during the reconstruction step, each hologram can be independently and accurately reconstructed for each wavelength. This procedure enables avoiding the superimposition of particles images that may be close to each other in multi-exposure holography. The feasibility is demonstrated by using a standard particle sizing reticle and shows the potential of this method for particle velocity measurement.

  12. Digital holography applications in ophthalmology, biometry, and optical trapping characterization

    NASA Astrophysics Data System (ADS)

    Potcoava, Mariana Camelia

    This dissertation combines various holographic techniques with application on the two- and three-dimensional imaging of ophthalmic tissue, fingerprints, and microsphere samples with micrometer resolution. Digital interference holography (DIH) uses scanned wavelengths to synthesize short-coherence interference tomographic images. We used DIH for in vitro imaging of human optic nerve head and retina. Tomographic images were produced by superposition of holograms. Holograms were obtained with a signal-to-noise ratio of approximately 50 dB. Optic nerve head characteristics (shape, diameter, cup depth, and cup width) were quantified with a few micron resolution (4.06--4.8mum). Multiple layers were distinguishable in cross-sectional images of the macula. To our knowledge, this is the first report of DIH use to image human macular and optic nerve tissue. Holographic phase microscopy is used to produce images of thin film patterns left by latent fingerprints. Two or more holographic phase images with different wavelengths are combined for optical phase unwrapping of images of patent prints. We demonstrated digital interference holography images of a plastic print, and latent prints. These demonstrations point to significant contributions to biometry by using digital interference holography to identify and quantify Level 1 (pattern), Level 2 (minutia points), and Level 3 (pores and ridge contours). Quantitative studies of physical and biological processes and precise non-contact manipulation of nanometer/micrometer trapped objects can be effectuated with nanometer accuracy due to the development of optical tweezers. A three-dimensional gradient trap is produced at the focus position of a high NA microscope objective. Particles are trapped axially and laterally due to the gradient force. The particle is confined in a potential well and the trap acts as a harmonic spring. The elastic constant or the stiffness along any axis is determined from the particle displacements in

  13. Image/video encryption using single shot digital holography

    NASA Astrophysics Data System (ADS)

    Li, Xiaoyu; Tang, Chen; Zhu, Xinjun; Li, Biyuan; Wang, Linlin; Yan, Xiusheng

    2015-05-01

    We propose a method for image/video encryption that combines double random-phase encoding in the Fresnel domain with a single shot digital holography. In this method, a complex object field can be reconstructed with only single frame hologram based on a constrained optimization method. The system without multiple shots and Fourier lens is simple, and allows to dynamically encrypt information. We test the proposed method on a computer simulated image, a grayscale image and a video in AVI format. Also we investigate the quality of the decryption process and the performance against noise attacks. The experimental results demonstrate the performance of the method.

  14. Insect Wing Displacement Measurement Using Digital Holography

    SciTech Connect

    Aguayo, Daniel D.; Mendoza Santoyo, Fernando; Torre I, Manuel H. de la; Caloca Mendez, Cristian I.

    2008-04-15

    Insects in flight have been studied with optical non destructive techniques with the purpose of using meaningful results in aerodynamics. With the availability of high resolution and large dynamic range CCD sensors the so called interferometric digital holographic technique was used to measure the surface displacement of in flight insect wings, such as butterflies. The wings were illuminated with a continuous wave Verdi laser at 532 nm, and observed with a CCD Pixelfly camera that acquire images at a rate of 11.5 frames per second at a resolution of 1392x1024 pixels and 12 Bit dynamic range. At this frame rate digital holograms of the wings were captured and processed in the usual manner, namely, each individual hologram is Fourier processed in order to find the amplitude and phase corresponding to the digital hologram. The wings displacement is obtained when subtraction between two digital holograms is performed for two different wings position, a feature applied to all consecutive frames recorded. The result of subtracting is seen as a wrapped phase fringe pattern directly related to the wing displacement. The experimental data for different butterfly flying conditions and exposure times are shown as wire mesh plots in a movie of the wings displacement.

  15. Reconstruction of off-axis lensless Fourier transform digital holograms based on angular spectrum theory

    NASA Astrophysics Data System (ADS)

    Wang, Guangjun; Wang, Huaying; Wang, Dayong; Xie, Jianjun; Zhao, Jie

    2007-12-01

    A simple holographic high-resolution imaging system without pre-magnification, which is based on off-axis lensless Fourier transform configuration, has been developed. Experimental investigations are performed on USAF resolution test target. The method based on angular spectrum theory for reconstructing lensless Fourier hologram is given. The reconstructed results of the same hologram at different reconstructing distances are presented for what is to our knowledge the first time. Approximate diffraction limited lateral resolution is achieved. The results show that the angular spectrum method has several advantages over more commonly used Fresnel transform method. Lossless reconstruction can be achieved for any numerical aperture holograms as long as the wave field is calculated at a special reconstructing distance, which is determined by the light wavelength and the chip size and the pixel size of the CCD sensor. This is very important for reconstructing an extremely large numerical aperture hologram. Frequency-domain spectrum filtering can be applied conveniently to remove the disturbance of zero-order. The reconstructed image wave field is accurate so long as the sampling theorem is not violated. The experimental results also demonstrate that for a high quality hologram, special image processing is unnecessary to obtain a high quality image.

  16. Three-dimensional information encryption and anticounterfeiting using digital holography.

    PubMed

    Shiu, Min-Tzung; Chew, Yang-Kun; Chan, Huang-Tian; Wong, Xin-Yu; Chang, Chi-Ching

    2015-01-01

    In this work, arbitrary micro phase-step digital holography with optical interferometry and digital image processing is utilized to obtain information about an image of a three-dimensional object and encrypting keys. Then, a computer-generated hologram is used for the purpose of holographic encryption. All information about the keys is required to perform the decryption, comprising the amplitude and phase distribution of the encrypting key, the distance of image reconstruction, zero-order term elimination, and twin-image term suppression. In addition to using identifiable information on different image planes and linear superposition processing hidden within the encrypted information, not only can we convey an important message, but we can also achieve anticounterfeiting. This approach retains the strictness of traditional holographic encryption and the convenience of digital holographic processing without image distortion. Therefore, this method provides better solutions to earlier methods for the security of the transmission of holographic information.

  17. Three-dimensional vibrometry of the human eardrum with stroboscopic lensless digital holography.

    PubMed

    Khaleghi, Morteza; Furlong, Cosme; Ravicz, Mike; Cheng, Jeffrey Tao; Rosowski, John J

    2015-05-01

    The eardrum or tympanic membrane (TM) transforms acoustic energy at the ear canal into mechanical motions of the ossicles. The acousto-mechanical transformer behavior of the TM is determined by its shape, three-dimensional (3-D) motion, and mechanical properties. We have developed an optoelectronic holographic system to measure the shape and 3-D sound-induced displacements of the TM. The shape of the TM is measured with dual-wavelength holographic contouring using a tunable near IR laser source with a central wavelength of 780 nm. 3-D components of sound-induced displacements of the TM are measured with the method of multiple sensitivity vectors using stroboscopic holographic interferometry. To accurately obtain sensitivity vectors, a new technique is developed and used in which the sensitivity vectors are obtained from the images of a specular sphere that is being illuminated from different directions. Shape and 3-D acoustically induced displacement components of cadaveric human TMs at several excitation frequencies are measured at more than one million points on its surface. A numerical rotation matrix is used to rotate the original Euclidean coordinate of the measuring system in order to obtain in-plane and out-of-plane motion components. Results show that in-plane components of motion are much smaller (<20%) than the out-of-plane motions’ components. PMID:25652791

  18. Optical voice recorder by off-axis digital holography.

    PubMed

    Matoba, Osamu; Inokuchi, Hiroki; Nitta, Kouichi; Awatsuji, Yasuhiro

    2014-11-15

    An optical voice recorder capable of recording and reproducing propagating sound waves by using off-axis digital holography, as well as quantitative visualization, is presented. Propagating sound waves temporally modulate the phase distribution of an impinging light wave via refractive index changes. This temporally modulated phase distribution is recorded in the form of digital holograms by a high-speed image sensor. After inverse propagation using Fresnel diffraction of a series of the recorded holograms, the temporal phase profile of the reconstructed object wave at each three-dimensional position can be used to reproduce the original sound wave. Experimental results using a tuning fork vibrating at 440 Hz and a human voice are presented to show the feasibility of the proposed method.

  19. Stimulated LIF studied using pulsed digital holography and modelling

    NASA Astrophysics Data System (ADS)

    Amer, Eynas; Stenvall, Jonas; Gren, Per; Sjödahl, Mikael

    2013-04-01

    A frequency tripled Q-switched Nd-YAG laser (wavelength 355 nm, pulse duration 12 ns) has been used to pump Coumarin 153 dye solved in ethanol. The laser induced fluorescence (LIF) spectrum has been recorded using a spectrometer at different dye concentrations. The frequency doubled 532 nm beam from the same laser is used as a probe beam to pass through the excited volume of the dye. Because of stimulated emission an increase of the probe (532 nm) beam energy is recorded and a reduction of the spontaneous fluorescence spectrum intensity is observed. A model was developed that approaches the trend of the gain as a function of the probe beam energy at low dye concentrations (less than 0.08 g/L). The stimulated LIF is further recorded using digital holography. Digital holograms were recorded for different dye concentrations using collimated laser light (532 nm) passed through the dye volume. Two holograms without and with the UV laser beam were recorded. Intensity maps were calculated from the recorded digital holograms and are used to calculate the gain of the green laser beam due to the stimulated fluorescence emission which is coupled to the dye concentration. The gain of the coherent 532 nm beam is seen in the intensity maps and its value is about 40% for a dye concentration of 0.32 g/L and decreases with the decrease of the dye concentration. The results show that pulsed digital holography can be coupled to the stimulated LIF effect for imaging fluorescent species.

  20. Digital polarization holography advancing geometrical phase optics.

    PubMed

    De Sio, Luciano; Roberts, David E; Liao, Zhi; Nersisyan, Sarik; Uskova, Olena; Wickboldt, Lloyd; Tabiryan, Nelson; Steeves, Diane M; Kimball, Brian R

    2016-08-01

    Geometrical phase or the fourth generation (4G) optics enables realization of optical components (lenses, prisms, gratings, spiral phase plates, etc.) by patterning the optical axis orientation in the plane of thin anisotropic films. Such components exhibit near 100% diffraction efficiency over a broadband of wavelengths. The films are obtained by coating liquid crystalline (LC) materials over substrates with patterned alignment conditions. Photo-anisotropic materials are used for producing desired alignment conditions at the substrate surface. We present and discuss here an opportunity of producing the widest variety of "free-form" 4G optical components with arbitrary spatial patterns of the optical anisotropy axis orientation with the aid of a digital spatial light polarization converter (DSLPC). The DSLPC is based on a reflective, high resolution spatial light modulator (SLM) combined with an "ad hoc" optical setup. The most attractive feature of the use of a DSLPC for photoalignment of nanometer thin photo-anisotropic coatings is that the orientation of the alignment layer, and therefore of the fabricated LC or LC polymer (LCP) components can be specified on a pixel-by-pixel basis with high spatial resolution. By varying the optical magnification or de-magnification the spatial resolution of the photoaligned layer can be adjusted to an optimum for each application. With a simple "click" it is possible to record different optical components as well as arbitrary patterns ranging from lenses to invisible labels and other transparent labels that reveal different images depending on the side from which they are viewed. PMID:27505793

  1. Deterministic phase encoding encryption in single shot digital holography

    NASA Astrophysics Data System (ADS)

    Chen, G.-L.; Yang, W.-K.; Wang, J. C.; Chang, C.-C.

    2008-11-01

    We demonstrate a deterministic phase-encoded encryption system based on the digital holography and adopted a lenticular lens array (LLA) sheet as a phase modulator. In the proposed scheme the holographic patterns of encrypted images are captured digitally by a digital CCD. This work also adopt a novel, simple and effective technique that is used to suppress numerically the major blurring caused by the zero-order image in the numerical reconstruction. The decryption key is acquired as a digital hologram, called the key hologram. Therefore, the retrieval of the original information can be achieved by multiplying the encrypted hologram with a numerical generated phase-encoded wave. The storage and transmission of all holograms can be carried out by all-digital means. Simulation and experimental results demonstrate that the proposed approach can be operated in single procedure only and represent the satisfactory decrypted image. Finally, rotating and shifting the LLA is applied to investigate the tolerance of decryption to demonstrate the feasibility in the holographic encryption, as well as can also be used to provide the higher security.

  2. The application of digital image plane holography technology to identify Chinese herbal medicine

    NASA Astrophysics Data System (ADS)

    Wang, Huaying; Guo, Zhongjia; Liao, Wei; Zhang, Zhihui

    2012-03-01

    In this paper, the imaging technology of digital image plane holography to identify the Chinese herbal medicine is studied. The optical experiment system of digital image plane holography which is the special case of pre-magnification digital holography was built. In the record system, one is an object light by using plane waves which illuminates the object, and the other one is recording hologram by using spherical light wave as reference light. There is a Micro objective lens behind the object. The second phase factor which caus ed by the Micro objective lens can be eliminated by choosing the proper position of the reference point source when digital image plane holography is recorded by spherical light. In this experiment, we use the Lygodium cells and Onion cells as the object. The experiment results with Lygodium cells and Onion cells show that digital image plane holography avoid the process of finding recording distance by using auto-focusing approach, and the phase information of the object can be reconstructed more accurately. The digital image plane holography is applied to the microscopic imaging of cells more effectively, and it is suit to apply for the identify of Chinese Herbal Medicine. And it promotes the application of digital holographic in practice.

  3. The application of digital image plane holography technology to identify Chinese herbal medicine

    NASA Astrophysics Data System (ADS)

    Wang, Huaying; Guo, Zhongjia; Liao, Wei; Zhang, Zhihui

    2011-11-01

    In this paper, the imaging technology of digital image plane holography to identify the Chinese herbal medicine is studied. The optical experiment system of digital image plane holography which is the special case of pre-magnification digital holography was built. In the record system, one is an object light by using plane waves which illuminates the object, and the other one is recording hologram by using spherical light wave as reference light. There is a Micro objective lens behind the object. The second phase factor which caus ed by the Micro objective lens can be eliminated by choosing the proper position of the reference point source when digital image plane holography is recorded by spherical light. In this experiment, we use the Lygodium cells and Onion cells as the object. The experiment results with Lygodium cells and Onion cells show that digital image plane holography avoid the process of finding recording distance by using auto-focusing approach, and the phase information of the object can be reconstructed more accurately. The digital image plane holography is applied to the microscopic imaging of cells more effectively, and it is suit to apply for the identify of Chinese Herbal Medicine. And it promotes the application of digital holographic in practice.

  4. Off-axis illumination direct-to-digital holography

    DOEpatents

    Thomas, Clarence E.; Price, Jeffery R.; Voelkl, Edgar; Hanson, Gregory R.

    2004-06-08

    Systems and methods are described for off-axis illumination direct-to-digital holography. A method of recording an off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis, includes: reflecting a reference beam from a reference mirror at a non-normal angle; reflecting an object beam from an object at an angle with respect to an optical axis defined by a focusing lens; focusing the reference beam and the object beam at a focal plane of a digital recorder to form the off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis; digitally recording the off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis; Fourier analyzing the recorded off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes by transforming axes of the recorded off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes in Fourier space to sit on top of a heterodyne carrier frequency defined as an angle between the reference beam and the object beam; applying a digital filter to cut off signals around an original origin; and then performing an inverse Fourier transform.

  5. Time-averaged photon-counting digital holography.

    PubMed

    Demoli, Nazif; Skenderović, Hrvoje; Stipčević, Mario

    2015-09-15

    Time-averaged holography has been using photo-emulsions (early stage) and digital photo-sensitive arrays (later) to record holograms. We extend the recording possibilities by utilizing a photon-counting camera, and we further investigate the possibility of obtaining accurate hologram reconstructions in rather severe experimental conditions. To achieve this, we derived an expression for fringe function comprising the main parameters affecting the hologram recording. Influence of the main parameters, namely the exposure time and the number of averaged holograms, is analyzed by simulations and experiments. It is demonstrated that taking long exposure times can be avoided by averaging over many holograms with the exposure times much shorter than the vibration cycle. Conditions in which signal-to-noise ratio in reconstructed holograms can be substantially increased are provided. PMID:26371907

  6. Coherence experiments in single-pixel digital holography.

    PubMed

    Liu, Jung-Ping; Guo, Chia-Hao; Hsiao, Wei-Jen; Poon, Ting-Chung; Tsang, Peter

    2015-05-15

    In optical scanning holography (OSH), the coherence properties of the acquired holograms depend on the single-pixel size, i.e., the active area of the photodetector. For the first time, to the best of our knowledge, we have demonstrated coherent, partial coherent, and incoherent three-dimensional (3D) imaging by experiment in such a single-pixel digital holographic recording system. We have found, for the incoherent mode of OSH, in which the detector of the largest active area is applied, the 3D location of a diffusely reflecting object can be successfully retrieved without speckle noise. For the partial coherent mode employing a smaller pixel size of the detector, significant speckles and randomly distributed bright spots appear among the reconstructed images. For the coherent mode of OSH when the size of the pixel is vanishingly small, the bright spots disappear. However, the speckle remains and the signal-to-noise ratio is low. PMID:26393741

  7. Objective shearing digital holography for removing aberration from optical system.

    PubMed

    Pan, Weiqing; Tian, Kehan; Zhang, Chuhang

    2015-09-01

    We propose a new digital holography based on the lateral shearing interference concept to remove the total aberrations from the reference wave, illumination wave, and the optical elements. It uses three mutually shifted image holograms of the object that are divided from each other to obtain phase differences. The phase aberration can be removed and the original sample phase can be reconstructed by the phase differences. Then, the influence of the stage moving imprecision on the reconstruction quality is analyzed. Optical experiments verified that the proposed method can totally remove the phase aberrations. As a result, the proposed method could be used for ultra-precise optical measurement through eliminating optical phase aberration to increase the measurement accuracy. PMID:26368865

  8. Digital Holography of Intracellular Dynamics to Probe Tissue Physiology

    PubMed Central

    Merrill, Daniel; An, Ran; Turek, John; Nolte, David D.

    2015-01-01

    Digital holography provides improved capabilities for imaging through dense tissue. Using a short-coherence source, the digital hologram recorded from backscattered light performs laser ranging that maintains fidelity of information acquired from depths much greater than possible by traditional imaging techniques. Biodynamic Imaging (BDI) is a developing technology for live-tissue imaging of up to a millimeter in depth that uses the hologram intensity fluctuations as label-free image contrast and can study tissue behavior in native microenvironments. In this paper BDI is used investigate the change in adhesion-dependent tissue response in 3D cultures. The results show that increasing density of cellular adhesions slows motion inside tissue and alters the response to cytoskeletal drugs. A clear signature of membrane fluctuations was observed in mid frequencies (0.1 – 1 Hz) that was enhanced by the application of cytochalasin-D that degrades the actin cortex inside the cell membrane. This enhancement feature is only observed in tissues that have formed adhesions, because cell pellets initially do not show this signature, but develop this signature only after incubation enables adhesions to form. PMID:25967027

  9. Digital holography of intracellular dynamics to probe tissue physiology.

    PubMed

    Merrill, Daniel; An, Ran; Turek, John; Nolte, David D

    2015-01-01

    Digital holography provides improved capabilities for imaging through dense tissue. Using a short-coherence source, the digital hologram recorded from backscattered light performs laser ranging that maintains fidelity of information acquired from depths much greater than possible by traditional imaging techniques. Biodynamic imaging (BDI) is a developing technology for live-tissue imaging of up to a millimeter in depth that uses the hologram intensity fluctuations as label-free image contrast and can study tissue behavior in native microenvironments. In this paper BDI is used to investigate the change in adhesion-dependent tissue response in 3D cultures. The results show that increasing density of cellular adhesions slows motion inside tissue and alters the response to cytoskeletal drugs. A clear signature of membrane fluctuations was observed in mid-frequencies (0.1-1 Hz) and was enhanced by the application of cytochalasin-D that degrades the actin cortex inside the cell membrane. This enhancement feature is only observed in tissues that have formed adhesions, because cell pellets initially do not show this signature, but develop this signature only after incubation enables adhesions to form.

  10. In vitro imaging of ophthalmic tissue by digital interference holography

    NASA Astrophysics Data System (ADS)

    Potcoava, Mariana C.; Kay, Christine N.; Kim, Myung K.; Richards, David W.

    2010-01-01

    We used digital interference holography (DIH) for in vitro imaging of human optic nerve head and retina. Samples of peripheral retina, macula, and optic nerve head from two formaldehyde-preserved human eyes were dissected and mounted onto slides. Holograms were captured by a monochrome CCD camera (Sony XC-ST50, with 780 × 640 pixels and pixel size of ∼9 µm). Light source was a solid-state pumped dye laser with tunable wavelength range of 560-605 nm. Using about 50 wavelengths in this band, holograms were obtained and numerically reconstructed using custom software based on NI LabView. Tomographic images were produced by superposition of holograms. Holograms of all tissue samples were obtained with a signal-to-noise ratio of approximately 50 dB. Optic nerve head characteristics (shape, diameter, cup depth, and cup width) were quantified with a few micron resolution (4.06-4.8 µm). Multiple layers were distinguishable in cross-sectional images of the macula. To our knowledge, this is the first report of DIH use to image human macular and optic nerve tissue. DIH has the potential to become a useful tool for researchers and clinicians in the diagnosis and treatment of many ocular diseases, including glaucoma and a variety of macular diseases.

  11. Digital reflection holography based systems development for MEMS testing

    NASA Astrophysics Data System (ADS)

    Singh, Vijay Raj; Liansheng, Sui; Asundi, Anand

    2010-05-01

    MEMS are tiny mechanical devices that are built onto semiconductor chips and are measured in micrometers and nanometers. Testing of MEMS device is an important part in carrying out their functional assessment and reliability analysis. Development of systems based on digital holography (DH) for MEMS inspection and characterization is presented in this paper. Two DH reflection systems, table-top and handheld types, are developed depending on the MEMS measurement requirements and their capabilities are presented. The methodologies for the systems are developed for 3D profile inspection and static & dynamic measurements, which is further integrated with in-house developed software that provides the measurement results in near real time. The applications of the developed systems are demonstrated for different MEMS devices for 3D profile inspection, static deformation/deflection measurements and vibration analysis. The developed systems are well suitable for the testing of MEMS and Microsystems samples, with full-field, static & dynamic inspection as well as to monitor micro-fabrication process.

  12. Measurement of rabbit eardrum vibration through stroboscopic digital holography

    NASA Astrophysics Data System (ADS)

    De Greef, Daniël; Dirckx, Joris J. J.

    2014-05-01

    In this work, we present a setup for high-power single shot stroboscopic digital holography and demonstrate it in an application on rabbit eardrum vibration measurement. The setup is able to make full-field time-resolved measurements of vibrating surfaces with a precision in the nanometer range in a broad frequency range. The height displacement of the measured object is visualized over the entire surface as a function of time. Vibration magnitude and phase maps can be extracted from these data, the latter proving to be very useful to reveal phase delays across the surface. Such deviations from modal motion indicate energy losses due to internal damping, in contrast to purely elastic mechanics. This is of great interest in middle ear mechanics and finite element modelling. In our setup, short laser pulses are fired at selected instants within the surface vibration period and are recorded by a CCD camera. The timing of the pulses and the exposure of the camera are synchronized to the vibration phase by a microprocessor. The high-power frequency-doubled Nd:YAG laser produces pulses containing up to 5 mJ of energy, which is amply sufficient to record single-shot holograms. As the laser pulse length is 8 ns and the smallest time step of the trigger electronics is 1 μs, vibration measurements of frequencies up to 250 kHz are achievable through this method, provided that the maximum vibration amplitude exceeds a few nanometers. In our application, middle ear mechanics, measuring frequencies extend from 5 Hz to 20 kHz. The experimental setup will be presented, as well as results of measurements on a stretched circular rubber membrane and a rabbit's eardrum. Two of the challenges when measuring biological tissues, such as the eardrum, are low reflectivity and fast dehydration. To increase reflectivity, a coating is applied and to counteract the undesirable effects of tissue dehydration, the measurement setup and software have been optimized for speed without compromising

  13. Measurement of rabbit eardrum vibration through stroboscopic digital holography

    SciTech Connect

    De Greef, Daniël; Dirckx, Joris J. J.

    2014-05-27

    In this work, we present a setup for high-power single shot stroboscopic digital holography and demonstrate it in an application on rabbit eardrum vibration measurement. The setup is able to make full-field time-resolved measurements of vibrating surfaces with a precision in the nanometer range in a broad frequency range. The height displacement of the measured object is visualized over the entire surface as a function of time. Vibration magnitude and phase maps can be extracted from these data, the latter proving to be very useful to reveal phase delays across the surface. Such deviations from modal motion indicate energy losses due to internal damping, in contrast to purely elastic mechanics. This is of great interest in middle ear mechanics and finite element modelling. In our setup, short laser pulses are fired at selected instants within the surface vibration period and are recorded by a CCD camera. The timing of the pulses and the exposure of the camera are synchronized to the vibration phase by a microprocessor. The high-power frequency-doubled Nd:YAG laser produces pulses containing up to 5 mJ of energy, which is amply sufficient to record single-shot holograms. As the laser pulse length is 8 ns and the smallest time step of the trigger electronics is 1 μs, vibration measurements of frequencies up to 250 kHz are achievable through this method, provided that the maximum vibration amplitude exceeds a few nanometers. In our application, middle ear mechanics, measuring frequencies extend from 5 Hz to 20 kHz. The experimental setup will be presented, as well as results of measurements on a stretched circular rubber membrane and a rabbit's eardrum. Two of the challenges when measuring biological tissues, such as the eardrum, are low reflectivity and fast dehydration. To increase reflectivity, a coating is applied and to counteract the undesirable effects of tissue dehydration, the measurement setup and software have been optimized for speed without compromising

  14. High-speed digital in-line holography as multipoint vibrometry to analyze vibrations of structures

    NASA Astrophysics Data System (ADS)

    Poittevin, J.; Picart, P.; Gautier, F.; Pezerat, C.

    2015-05-01

    Structural vibrations can be measured with optical digital holography. Such a method provides measurements with a very high spatial resolution and is a nonintrusive technique. This method is based on the interference between a reference laser beam and the field diffracted by the studied object. Using a high speed camera, it can also be implemented in the time domain to investigate non-stationary problems. In this paper, we present a recent investigation which shows that the high-speed digital holography is comparable with classical laser vibrometry. Furthermore, an experimental investigation of the vibratory field inside the Acoustic Black Hole is discussed. The principle of digital holography is explained and it is used here to provide a full field measurement of the velocity field at the extremity of the structure.

  15. Suppression of image autocorrelation artefacts in spectral domain optical coherence tomography and multiwave digital holography

    SciTech Connect

    Gelikonov, V M; Gelikonov, G V; Terpelov, D A; Shabanov, D V; Shilyagin, P A

    2012-05-31

    An improved method for suppressing image artefacts in spectral domain optical coherence tomography (SD OCT) and multiwave digital holography, caused by the influence of coherent noise in the course of successive registration of an autocorrelation component and informative signal is reported. The method allows complete suppression of all types of coherent noises, provided that the sample of values used to record the autocorrelation component satisfies the conditions of Kotelnikov's theorem: in SD OCT - for the transverse structure of the studied medium, in multiwave digital holography - for the envelop function of the radiation source frequency tuning spectrum.

  16. High-resolution, lensless endoscope based on digital scanning through a multimode optical fiber

    PubMed Central

    Papadopoulos, Ioannis N.; Farahi, Salma; Moser, Christophe; Psaltis, Demetri

    2013-01-01

    We propose and experimentally demonstrate an ultra-thin rigid endoscope (450 μm diameter) based on a passive multimode optical fiber. We use digital phase conjugation to overcome the modal scrambling of the fiber to tightly focus and scan the laser light at its distal end. By exploiting the maximum number of modes available, sub-micron resolution, high quality fluorescence images of neuronal cells were acquired. The imaging system is evaluated in terms of fluorescence collection efficiency, resolution and field of view. The small diameter of the proposed endoscope, along with its high quality images offer an opportunity for minimally invasive medical endoscopic imaging and diagnosis based on cellular phenotype via direct tissue penetration. PMID:23411747

  17. Digital holography with electron wave: measuring into the nanoworld

    NASA Astrophysics Data System (ADS)

    Mendoza Santoyo, Fernando; Voelkl, Edgar

    2016-04-01

    Dennis Gabor invented Holography in 1949. His main concern at the time was centered on the spherical aberration correction in the recently created electron microscopes, especially after O. Scherzer had shown mathematically that round electron optical lenses always have a positive spherical aberration coefficient and the mechanical requirements for minimizing the spherical aberration were too high to allow for atomic resolution. At the time the lack of coherent electron sources meant that in-line holography was developed using quasi-coherent light sources. As such Holography did not produce scientific good enough results to be considered a must use tool. In 1956, G. Moellenstedt invented a device called a wire-biprism that allowed the object and reference beams to be combined in an off-axis configuration. The invention of the laser at the end of the 1950s gave a great leap to Holography since this light source was highly coherent and hence led to the invention of Holographic Interferometry during the first lustrum of the 1960s. This new discipline in the Optics field has successfully evolved to become a trusted tool in a wide variety of areas. Coherent electron sources were made available only by the late 1970s, a fact that gave an outstanding impulse to electron holography so that today nanomaterials and structures belonging to a wide variety of subjects can be characterized in regards to their physical and mechanical parameters. This invited paper will present and discuss electron holography's state of the art applications to study the shape of nanoparticles and bacteria, and the qualitative and quantitative study of magnetic and electric fields produced by novel nano-structures.

  18. Direct inversion of digital 3D Fraunhofer holography maps

    NASA Astrophysics Data System (ADS)

    Podorov, Sergei G.; Förster, Eckhart

    2016-01-01

    The Differential Fourier Holography (DFH) gives an exact mathematical solution of the inverse problem of diffraction in the Fraunhofer regime. After the first publication [1] the Differential Fourier Holography was successfully applied in many experiments to obtain amplitude and phase information about two-dimensional (2D) images. In this article we demonstrate numerically the possibility to apply the DFH also for investigation of unknown 3D Objects. The first simulation is made for a double-spiral structure plus a line as a reference object.

  19. Optimization of a lensless digital holographic otoscope system for transient measurements of the human tympanic membrane

    PubMed Central

    Dobrev, I.; Furlong, C.; Cheng, J. T.; Rosowski, J. J.

    2014-01-01

    In this paper, we propose a multi-pulsed double exposure (MPDE) acquisition method to quantify in full-field-of-view the transient (i.e., >10 kHz) acoustically induced nanometer scale displacements of the human tympanic membrane (TM or eardrum). The method takes advantage of the geometrical linearity and repeatability of the TM displacements to enable high-speed measurements with a conventional camera (i.e., <20 fps). The MPDE is implemented on a previously developed digital holographic system (DHS) to enhance its measurement capabilities, at a minimum cost, while avoiding constraints imposed by the spatial resolutions and dimensions of high-speed (i.e., >50 kfps) cameras. To our knowledge, there is currently no existing system to provide such capabilities for the study of the human TM. The combination of high temporal (i.e., >50 kHz) and spatial (i.e., >500k data points) resolutions enables measurements of the temporal and frequency response of all points across the surface of the TM simultaneously. The repeatability and accuracy of the MPDE method are verified against a Laser Doppler Vibrometer (LDV) on both artificial membranes and ex-vivo human TMs that are acoustically excited with a sharp (i.e., <100 μs duration) click. The measuring capabilities of the DHS, enhanced by the MPDE acquisition method, allow for quantification of spatially dependent motion parameters of the TM, such as modal frequencies, time constants, as well as inferring local material properties. PMID:25780271

  20. Investigations of cloud microphysical response to mixing using digital holography

    NASA Astrophysics Data System (ADS)

    Beals, Matthew Jacob

    Cloud edge mixing plays an important role in the life cycle and development of clouds. Entrainment of subsaturated air affects the cloud at the microscale, altering the number density and size distribution of its droplets. The resulting effect is determined by two timescales: the time required for the mixing event to complete, and the time required for the droplets to adjust to their new environment. If mixing is rapid, evaporation of droplets is uniform and said to be homogeneous in nature. In contrast, slow mixing (compared to the adjustment timescale) results in the droplets adjusting to the transient state of the mixture, producing an inhomogeneous result. Studying this process in real clouds involves the use of airborne optical instruments capable of measuring clouds at the 'single particle' level. Single particle resolution allows for direct measurement of the droplet size distribution. This is in contrast to other 'bulk' methods (i.e. hot-wire probes, lidar, radar) which measure a higher order moment of the distribution and require assumptions about the distribution shape to compute a size distribution. The sampling strategy of current optical instruments requires them to integrate over a path tens to hundreds of meters to form a single size distribution. This is much larger than typical mixing scales (which can extend down to the order of centimeters), resulting in difficulties resolving mixing signatures. The Holodec is an optical particle instrument that uses digital holography to record discrete, local volumes of droplets. This method allows for statistically significant size distributions to be calculated for centimeter scale volumes, allowing for full resolution at the scales important to the mixing process. The hologram also records the three dimensional position of all particles within the volume, allowing for the spatial structure of the cloud volume to be studied. Both of these features represent a new and unique view into the mixing problem. In

  1. Trajectory and velocity measurement of a particle in spray by digital holography

    SciTech Connect

    Lue Qieni; Chen Yiliang; Yuan Rui; Ge Baozhen; Gao Yan; Zhang Yimo

    2009-12-20

    We present a method for the trajectory and the velocity measurement of a particle in spray by digital holography. Based on multiple exposure digital in-line holography, a sequence of digital holograms of a dynamic spray particle field at different times are recorded with a CW laser and a high-speed CCD. The time evolution of the serial positions of particles, i.e., the motion trajectories of the particles, is obtained by numerically reconstructing the synthetic hologram of a sequence of digital holograms. The center coordinate (x,y) of each particle image can be extracted using a Hough transform and subpixel precision computing, and the velocity of an individual particle can also be obtained, which is then applied to measuring the velocity of diesel spray and alcohol spray. The research shows that the method presented in this paper for measuring spray field is feasible.

  2. Coded aperture correlation holography-a new type of incoherent digital holograms.

    PubMed

    Vijayakumar, A; Kashter, Yuval; Kelner, Roy; Rosen, Joseph

    2016-05-30

    We propose and demonstrate a new concept of incoherent digital holography termed coded aperture correlation holography (COACH). In COACH, the hologram of an object is formed by the interference of light diffracted from the object, with light diffracted from the same object, but that passes through a coded phase mask (CPM). Another hologram is recorded for a point object, under identical conditions and with the same CPM. This hologram is called the point spread function (PSF) hologram. The reconstructed image is obtained by correlating the object hologram with the PSF hologram. The image reconstruction of multiplane object using COACH was compared with that of other equivalent imaging systems, and has been found to possess a higher axial resolution compared to Fresnel incoherent correlation holography.

  3. Coded aperture correlation holography-a new type of incoherent digital holograms.

    PubMed

    Vijayakumar, A; Kashter, Yuval; Kelner, Roy; Rosen, Joseph

    2016-05-30

    We propose and demonstrate a new concept of incoherent digital holography termed coded aperture correlation holography (COACH). In COACH, the hologram of an object is formed by the interference of light diffracted from the object, with light diffracted from the same object, but that passes through a coded phase mask (CPM). Another hologram is recorded for a point object, under identical conditions and with the same CPM. This hologram is called the point spread function (PSF) hologram. The reconstructed image is obtained by correlating the object hologram with the PSF hologram. The image reconstruction of multiplane object using COACH was compared with that of other equivalent imaging systems, and has been found to possess a higher axial resolution compared to Fresnel incoherent correlation holography. PMID:27410157

  4. A novel method for identifying the order of interference using phase-shifting digital holography.

    PubMed

    Sokkar, T Z N; El-Farahaty, K A; Ramadan, W A; Wahba, H H; Raslan, M I; Hamza, A A

    2016-04-01

    In this paper, we introduced a mathematical method for measuring the optical path length differences (OPDs), which is suitable for large OPD values where the fringes connections are difficult to detect. The proposed method is based on varying the width of the fringes, without changing the wavelength of the used coherent source. Also, in this work, we discussed the need for such method in off-axis phase-shifting digital holography. Low-resolution off-axis holograms failed to detect the correct interference order. In general, off-axis phase-shifting digital holography is limited by the resolution of the captured holograms. The results obtained using our proposed technique were compared to the results obtained using off-axis phase-shifting digital holograms and conventional two-beam interferometry. Holograms were given for illustration. PMID:26588671

  5. Applications of digital holography in visualized measurement of acoustic and flow fields

    NASA Astrophysics Data System (ADS)

    Zhao, Jianlin; Li, Enpu; Sun, Weiwei; Di, Jianglei

    2010-03-01

    Digital holography allows recording the hologram using digitally imaging devices such as CCD, and reconstructing the holographic image by numerically simulating the diffraction of the hologram. Its main advantages are by which one can directly obtain the complex amplitude distribution of the object field, so that more impersonally measure the detail information of the object field, such as the distribution of the refractive index changing in crystals induced by light irradiation, deformation of the object surface, particle distribution, as well as acoustic field, flow field and temperature distribution in air. In this paper, we summarize the principle and some of our experimental results on the applications of digital holography in visualized measurement of acoustic standing wave (acoustic levitation field), plasma plume and water flow (Karman vortex street) fields.

  6. Applications of digital holography in visualized measurement of acoustic and flow fields

    NASA Astrophysics Data System (ADS)

    Zhao, Jianlin; Li, Enpu; Sun, Weiwei; di, Jianglei

    2009-12-01

    Digital holography allows recording the hologram using digitally imaging devices such as CCD, and reconstructing the holographic image by numerically simulating the diffraction of the hologram. Its main advantages are by which one can directly obtain the complex amplitude distribution of the object field, so that more impersonally measure the detail information of the object field, such as the distribution of the refractive index changing in crystals induced by light irradiation, deformation of the object surface, particle distribution, as well as acoustic field, flow field and temperature distribution in air. In this paper, we summarize the principle and some of our experimental results on the applications of digital holography in visualized measurement of acoustic standing wave (acoustic levitation field), plasma plume and water flow (Karman vortex street) fields.

  7. Security enhanced optical one-time password authentication method by using digital holography

    NASA Astrophysics Data System (ADS)

    Gil, Sang Keun; Jeon, Seok Hee; Jeong, Jong Rae

    2015-03-01

    We propose a new optical one-time password(OTP) authentication method by using digital holography, which enhances security strength in the cryptosystem compared to the conventional electronic OTP method. In this paper, a challenge-response optical OTP authentication based on two-factor authentication is presented by 2-step quadrature phase-shifting digital holography using orthogonal polarization, and two-way authentication is also performed using the challenge-response handshake in both directions. The ID (identification), PW (password) and OTP information are encrypted with a shared key by applying phase-shifting digital holography, and these encrypted information are verified each other by the shared key. Because the encrypted digital holograms which are transmitted to the other party are expressed as random distribution, it guards against a replay attack and results in higher security level. Optically, encrypted digital hologram in our method is Fourier transform hologram and is recorded on CCD with 256 gray-level quantized intensities. The proposed method has an advantage that it does not need a time-synchronized OTP and can be applied to various security services. Computer experiments show that the proposed method is suitable for high secure OTP authentication.

  8. Experimental imaging research on continuous-wave terahertz in-line digital holography

    NASA Astrophysics Data System (ADS)

    Huang, Haochong; Wang, Dayong; Rong, Lu; Wang, Yunxin

    2014-09-01

    The terahertz (THz) imaging is an advanced technique on the basis of the unique characteristics of terahertz radiation. Due to its noncontact, non-invasive and high-resolution capabilities, it has already shown great application prospects in biomedical observation, sample measurement, and quality control. The continuous-wave terahertz in-line digital holography is a combination of terahertz technology and in-line digital holography of which the source is a continuous-wave terahertz laser. Over the past decade, many researchers used different terahertz sources and detectors to undertake experiments. In this paper, the pre-process of the hologram is accomplished after the holograms' recording process because of the negative pixels in the pyroelectric detector and the air vibration caused by the chopper inside the camera. To improve the quality of images, the phase retrieval algorithm is applied to eliminate the twin images. In the experiment, the pin which terahertz wave can't penetrate and the TPX slice carved letters "THz" are chosen for the samples. The amplitude and phase images of samples are obtained and the twin image and noise in the reconstructed images are suppressed. The results validate the feasibility of the terahertz in-line digital holographic imaging technique. This work also shows the terahertz in-line digital holography technique's prospects in materials science and biological samples' detection.

  9. Real-time visualization and analysis of airflow field by use of digital holography

    NASA Astrophysics Data System (ADS)

    Di, Jianglei; Wu, Bingjing; Chen, Xin; Liu, Junjiang; Wang, Jun; Zhao, Jianlin

    2013-04-01

    The measurement and analysis of airflow field is very important in fluid dynamics. For airflow, smoke particles can be added to visually observe the turbulence phenomena by particle tracking technology, but the effect of smoke particles to follow the high speed airflow will reduce the measurement accuracy. In recent years, with the advantage of non-contact, nondestructive, fast and full-field measurement, digital holography has been widely applied in many fields, such as deformation and vibration analysis, particle characterization, refractive index measurement, and so on. In this paper, we present a method to measure the airflow field by use of digital holography. A small wind tunnel model made of acrylic glass is built to control the velocity and direction of airflow. Different shapes of samples such as aircraft wing and cylinder are placed in the wind tunnel model to produce different forms of flow field. With a Mach-Zehnder interferometer setup, a series of digital holograms carrying the information of airflow filed distributions in different states are recorded by CCD camera and corresponding holographic images are numerically reconstructed from the holograms by computer. Then we can conveniently obtain the velocity or pressure information of the airflow deduced from the quantitative phase information of holographic images and visually display the airflow filed and its evolution in the form of a movie. The theory and experiment results show that digital holography is a robust and feasible approach for real-time visualization and analysis of airflow field.

  10. Direct inversion of digital 3D Fraunhofer holography maps.

    PubMed

    Podorov, Sergei G; Förster, Eckhart

    2016-01-20

    Differential Fourier holography (DFH) gives an exact mathematical solution of the inverse problem of diffraction in the Fraunhofer regime. After the first publication [Opt. Express15, 9954 (2007)], DFH was successfully applied in many experiments to obtain amplitude and phase information about two-dimensional images. In this paper, we demonstrate numerically the possibility to apply DFH also for investigation of unknown three-dimensional objects. The first simulation is made for a double-spiral structure plus a line as a reference object.

  11. Improvement of color reproduction in color digital holography by using spectral estimation technique.

    PubMed

    Xia, Peng; Shimozato, Yuki; Ito, Yasunori; Tahara, Tatsuki; Kakue, Takashi; Awatsuji, Yasuhiro; Nishio, Kenzo; Ura, Shogo; Kubota, Toshihiro; Matoba, Osamu

    2011-12-01

    We propose a color digital holography by using spectral estimation technique to improve the color reproduction of objects. In conventional color digital holography, there is insufficient spectral information in holograms, and the color of the reconstructed images depend on only reflectances at three discrete wavelengths used in the recording of holograms. Therefore the color-composite image of the three reconstructed images is not accurate in color reproduction. However, in our proposed method, the spectral estimation technique was applied, which has been reported in multispectral imaging. According to the spectral estimation technique, the continuous spectrum of object can be estimated and the color reproduction is improved. The effectiveness of the proposed method was confirmed by a numerical simulation and an experiment, and, in the results, the average color differences are decreased from 35.81 to 7.88 and from 43.60 to 25.28, respectively. PMID:22193005

  12. Effect of changing speckles in digital holography on measurements of static and vibratory displacements.

    PubMed

    Stetson, Karl A

    2016-06-01

    This paper presents a study of speckle effects in measurements of static and vibratory displacements by digital holography. Such effects are shown to arise from changes in speckle fields that often occur between holographic recordings. These may be between recording holograms before and after static deformations or changes in sets of holograms recorded for vibration measurement. If the images do not change between such recordings, the effects appear to be limited mainly to round-off errors. PMID:27411207

  13. Digital Holography for in Situ Real-Time Measurement of Plasma-Facing-Component Erosion

    SciTech Connect

    ThomasJr., C. E.; Granstedt, E. M.; Biewer, Theodore M; Baylor, Larry R; Combs, Stephen Kirk; Meitner, Steven J; Hillis, Donald Lee; Majeski, R.; Kaita, R.

    2014-01-01

    In situ, real time measurement of net plasma-facing-component (PFC) erosion/deposition in a real plasma device is challenging due to the need for good spatial and temporal resolution, sufficient sensitivity, and immunity to fringe-jump errors. Design of a high-sensitivity, potentially high-speed, dual-wavelength CO2 laser digital holography system (nominally immune to fringe jumps) for PFC erosion measurement is discussed.

  14. Microfluidic system based on the digital holography microscope for analysis of motile sperm

    NASA Astrophysics Data System (ADS)

    Di Caprio, G.; Coppola, G.; Grilli, S.; Ferraro, P.; Puglisi, R.; Balduzzi, D.; Galli, A.

    2009-06-01

    Digital holography (DH) has been employed in the retrieval of three dimensional images of bull's sperm heads. The system allows a three dimensional analysis of the sperm morphology by means of a Digital Holographic Microscope (DHM). Microscopic holography measurements are performed by projecting a magnified image of a microscopic hologram plane onto a CCD plane. This could constitute the basis of an alternative method for the zoothecnic industry aimed at the investigation of morphological features and the sorting of the motile sperm cells. Indeed, one of the main advantages of digital holography consists in its full non-invasivity and in the capability of investigating the shape of the sperm cells without altering their characteristics. In particular the proposed technique could be applied to investigate the frequency of aberrant spermatozoa. Until now, in fact, such industrial investigations have been mainly performed by means of specific painting probes: unfortunately this technique dramatically reduces the vitality of the sperm cells and can even cause chromosome aberration, making them useless for the zootechnical applications.

  15. Optical design of cipher block chaining (CBC) encryption mode by using digital holography

    NASA Astrophysics Data System (ADS)

    Gil, Sang Keun; Jeon, Seok Hee; Jung, Jong Rae; Kim, Nam

    2016-03-01

    We propose an optical design of cipher block chaining (CBC) encryption by using digital holographic technique, which has higher security than the conventional electronic method because of the analog-type randomized cipher text with 2-D array. In this paper, an optical design of CBC encryption mode is implemented by 2-step quadrature phase-shifting digital holographic encryption technique using orthogonal polarization. A block of plain text is encrypted with the encryption key by applying 2-step phase-shifting digital holography, and it is changed into cipher text blocks which are digital holograms. These ciphered digital holograms with the encrypted information are Fourier transform holograms and are recorded on CCDs with 256 gray levels quantized intensities. The decryption is computed by these encrypted digital holograms of cipher texts, the same encryption key and the previous cipher text. Results of computer simulations are presented to verify that the proposed method shows the feasibility in the high secure CBC encryption system.

  16. Multilevel recording of complex amplitude data pages in a holographic data storage system using digital holography.

    PubMed

    Nobukawa, Teruyoshi; Nomura, Takanori

    2016-09-01

    A holographic data storage system using digital holography is proposed to record and retrieve multilevel complex amplitude data pages. Digital holographic techniques are capable of modulating and detecting complex amplitude distribution using current electronic devices. These techniques allow the development of a simple, compact, and stable holographic storage system that mainly consists of a single phase-only spatial light modulator and an image sensor. As a proof-of-principle experiment, complex amplitude data pages with binary amplitude and four-level phase are recorded and retrieved. Experimental results show the feasibility of the proposed holographic data storage system. PMID:27607703

  17. Digital Stroboscopic Holography Setup for Deformation Measurement at Both Quasi-Static and Acoustic Frequencies

    NASA Astrophysics Data System (ADS)

    De Greef, Daniël; Soons, Joris; Dirckx, Joris J. J.

    2014-10-01

    A setup for digital stroboscopic holography that combines the advantages of full-field digital holographic interferometry with a high temporal resolution is presented. The setup can be used to identify and visualize complicated vibrational patterns with nanometer amplitudes, ranging from quasi-static to high frequency vibrations. By using a high-energy pulsed laser, single-shot holograms can be recorded and stability issues are avoided. Results are presented for an acoustically stimulated rubber membrane and the technique is evaluated by means of an accuracy and a repeatability test. The presented technique offers wide application possibilities in areas such as biomechanics and industrial testing.

  18. Multilevel recording of complex amplitude data pages in a holographic data storage system using digital holography.

    PubMed

    Nobukawa, Teruyoshi; Nomura, Takanori

    2016-09-01

    A holographic data storage system using digital holography is proposed to record and retrieve multilevel complex amplitude data pages. Digital holographic techniques are capable of modulating and detecting complex amplitude distribution using current electronic devices. These techniques allow the development of a simple, compact, and stable holographic storage system that mainly consists of a single phase-only spatial light modulator and an image sensor. As a proof-of-principle experiment, complex amplitude data pages with binary amplitude and four-level phase are recorded and retrieved. Experimental results show the feasibility of the proposed holographic data storage system.

  19. Parallel phase-shifting digital holography and its application to high-speed 3D imaging of dynamic object

    NASA Astrophysics Data System (ADS)

    Awatsuji, Yasuhiro; Xia, Peng; Wang, Yexin; Matoba, Osamu

    2016-03-01

    Digital holography is a technique of 3D measurement of object. The technique uses an image sensor to record the interference fringe image containing the complex amplitude of object, and numerically reconstructs the complex amplitude by computer. Parallel phase-shifting digital holography is capable of accurate 3D measurement of dynamic object. This is because this technique can reconstruct the complex amplitude of object, on which the undesired images are not superimposed, form a single hologram. The undesired images are the non-diffraction wave and the conjugate image which are associated with holography. In parallel phase-shifting digital holography, a hologram, whose phase of the reference wave is spatially and periodically shifted every other pixel, is recorded to obtain complex amplitude of object by single-shot exposure. The recorded hologram is decomposed into multiple holograms required for phase-shifting digital holography. The complex amplitude of the object is free from the undesired images is reconstructed from the multiple holograms. To validate parallel phase-shifting digital holography, a high-speed parallel phase-shifting digital holography system was constructed. The system consists of a Mach-Zehnder interferometer, a continuous-wave laser, and a high-speed polarization imaging camera. Phase motion picture of dynamic air flow sprayed from a nozzle was recorded at 180,000 frames per second (FPS) have been recorded by the system. Also phase motion picture of dynamic air induced by discharge between two electrodes has been recorded at 1,000,000 FPS, when high voltage was applied between the electrodes.

  20. Study on effects of organic solvents on Hela cells by digital holography

    NASA Astrophysics Data System (ADS)

    Ouyang, Liting; Wang, Dayong; Wang, Yunxin; Wang, Xinlong; Marx, Lisa

    2012-11-01

    In the anticancer research with traditional Chinese medicine, many medicinally effective components can only dissolve in higher polar organic solvents, such as ethanol, dimethyl sulfoxide (DMSO) etc. However, organic solvents may directly interfere with the accuracy of therapeutic efficacy evaluation. Therefore the study on effects of organic solvents with different concentrations on Hela cells is of great significance. The digital holography is a non-destructive and non-contact method to image the transparent sample without staining and with the high precision and high resolution. In this paper, the digital holography is proposed to replace the methyl-thiazol-tetrazolium (MTT) or the Giemsa dye method. Based on the pre-magnification off-axis Fresnel digital holographic theory, an inverted microscopy system is built to obtain the phase-contrast images of the Hela cells, which are added different concentrations of organic solvents. Compared to the control group, there is significantly differences with the shapes of Hela cells with different organic solvents. The size of cell with ethanol 25% is no significantly difference with the control group. But the sizes of cells in the solutions with ethanol 12.5% and 50% are smaller than the control group. Next, the sizes of cells in the solutions with DMSO 12.5%, 25% and 50% are great smaller, compared with the control group. The results show that the digital holography has high practical value in detecting the changes in the shape of cells and is helpful in the choice of organic solvents for further apoptosis study.

  1. Formulation of the rotational transformation of wave fields and their application to digital holography.

    PubMed

    Matsushima, Kyoji

    2008-07-01

    Rotational transformation based on coordinate rotation in Fourier space is a useful technique for simulating wave field propagation between nonparallel planes. This technique is characterized by fast computation because the transformation only requires executing a fast Fourier transform twice and a single interpolation. It is proved that the formula of the rotational transformation mathematically satisfies the Helmholtz equation. Moreover, to verify the formulation and its usefulness in wave optics, it is also demonstrated that the transformation makes it possible to reconstruct an image on arbitrarily tilted planes from a wave field captured experimentally by using digital holography.

  2. Optical encryption of unlimited-size images based on ptychographic scanning digital holography.

    PubMed

    Gao, Qiankun; Wang, Yali; Li, Tuo; Shi, Yishi

    2014-07-20

    The ptychographic scanning operation is introduced into digital holography to expand the field-of-view (FOV). An optical image encryption method based on this technique is further proposed and analyzed. The plaintext is moved sequentially in the way of ptychographic scanning and corresponding pairs of phase-shifted interferograms are recorded as ciphertexts. Then the holographic processing and the ptychographic iterative reconstruction are both employed to retrieve the plaintext. Numerical experiments demonstrate that the proposed system possesses high security level and wide FOV. The proposed method might also be used for other potential applications, such as three-dimensional information encryption and image hiding.

  3. Wavelength scanning digital interference holography for high-resolution ophthalmic imaging

    NASA Astrophysics Data System (ADS)

    Potcoava, Mariana C.; Kim, M. K.; Kay, Christine N.

    2009-02-01

    An improved digital interference holography (DIH) technique suitable for fundus images is proposed. This technique incorporates a dispersion compensation algorithm to compensate for the unknown axial length of the eye. Using this instrument we acquired successfully tomographic fundus images in human eye with narrow axial resolution less than 5μm. The optic nerve head together with the surrounding retinal vasculature were constructed. We were able to quantify a depth of 84μm between the retinal fiber and the retinal pigmented epithelium layers. DIH provides high resolution 3D information which could potentially aid in guiding glaucoma diagnosis and treatment.

  4. Fast extended focused imaging in digital holography using a graphics processing unit.

    PubMed

    Wang, Le; Zhao, Jianlin; Di, Jianglei; Jiang, Hongzhen

    2011-05-01

    We present a simple and effective method for reconstructing extended focused images in digital holography using a graphics processing unit (GPU). The Fresnel transform method is simplified by an algorithm named fast Fourier transform pruning with frequency shift. Then the pixel size consistency problem is solved by coordinate transformation and combining the subpixel resampling and the fast Fourier transform pruning with frequency shift. With the assistance of the GPU, we implemented an improved parallel version of this method, which obtained about a 300-500-fold speedup compared with central processing unit codes.

  5. Developments in digital in-line holography enable validated measurement of 3D particle field dynamics.

    SciTech Connect

    Guildenbecher, Daniel Robert

    2013-12-01

    Digital in-line holography is an optical technique which can be applied to measure the size, three-dimensional position, and three-component velocity of disperse particle fields. This work summarizes recent developments at Sandia National Laboratories focused on improvement in measurement accuracy, experimental validation, and applications to multiphase flows. New routines are presented which reduce the uncertainty in measured position along the optical axis to a fraction of the particle diameter. Furthermore, application to liquid atomization highlights the ability to measure complex, three-dimensional structures. Finally, investigation of particles traveling at near sonic conditions prove accuracy despite significant experimental noise due to shock-waves.

  6. Detection of micromechanical deformation under rigid body displacement using twin-pulsed 3D digital holography

    NASA Astrophysics Data System (ADS)

    Perez-Lopez, Carlos; Hernandez-Montes, Maria del Socorro; Mendoza-Santoyo, Fernando

    2005-02-01

    Twin-pulsed digital holography in its 3D set up is used to recover exclusively the micro-mechanical deformation of an object. The test object is allowed to have rigid body movements such as rotation and translation, with the result that the fringe patterns contain information of the latter and the object deformation, a feature that may significantly modify the interpretation of the results. Experimental results from a flat metal plate subject to micro stress and a displacement in the x-z plane are presented to demonstrate that using this optical method it is possible to recover exclusively the contribution of the micro stress.

  7. Design of a digital holography system for PFC erosion measurements on Proto-MPEX

    NASA Astrophysics Data System (ADS)

    Thomas, C. E. Tommy; Biewer, T. M.; Baylor, L. R.; Combs, S. K.; Meitner, S. J.; Rapp, J.; Hillis, D. L.; Granstedt, E. M.; Majeski, R.; Kaita, R.

    2016-11-01

    A project has been started at ORNL to develop a dual-wavelength digital holography system for plasma facing component erosion measurements on prototype material plasma exposure experiment. Such a system will allow in situ real-time measurements of component erosion. Initially the system will be developed with one laser, and first experimental laboratory measurements will be made with the single laser system. In the second year of development, a second CO2 laser will be added and measurements with the dual wavelength system will begin. Adding the second wavelength allows measurements at a much longer synthetic wavelength.

  8. Proposal for underwater structural analysis using the techniques of ESPI and digital holography

    NASA Astrophysics Data System (ADS)

    Valin Rivera, Jose Luis; Monteiro, J. M.; Lopes, H. M.; Vaz, M. A. P.; Palacios, Francisco; Gonçalves, Edison; del Pino, Gilberto Garcia; Pérez, Jorge Ricardo

    2009-11-01

    The purpose of this article is to study the application of the holographic interferometry techniques in the structural analysis of submarine environment. These techniques are widely used today, with applications in many areas. Nevertheless, its application in submarine environments presents some challenges. The application of two techniques, electronic speckle pattern interferometry (ESPI) and digital holography, comparison of advantages and disadvantages of each of them is presented. A brief study is done on the influence of water properties and the optical effects due to suspended particles as well as possible solutions to minimize these problems.

  9. Three-wavelength digital holography using spatial frequency-division multiplexing and dual reference arms

    NASA Astrophysics Data System (ADS)

    Tahara, Tatsuki; Takeshita, Shingo; Morimoto, Kenta; Kaku, Toru; Arai, Yasuhiko

    2016-03-01

    We propose single-shot multiwavelength digital holography using a monochromatic image sensor and dual reference arms. Multiple wavelength information is multiplexed on the monochromatic image sensor plane in the space domain and is separated in the spatial frequency domain by utilizing the difference between the spatial frequencies of interference fringes at respective wavelengths. The recordable spatial bandwidth that is utilized for object waves is extended by using dual reference arms in comparison with that using a single reference arm. Both the three-dimensional and three-wavelength information of an object were recorded and reconstructed without the crosstalk between object waves with multiple wavelengths.

  10. Apparatus for direct-to-digital spatially-heterodyned holography

    DOEpatents

    Thomas, Clarence E.; Hanson, Gregory R.

    2006-12-12

    An apparatus operable to record a spatially low-frequency heterodyne hologram including spatially heterodyne fringes for Fourier analysis includes: a laser; a beamsplitter optically coupled to the laser; an object optically coupled to the beamsplitter; a focusing lens optically coupled to both the beamsplitter and the object; a digital recorder optically coupled to the focusing lens; and a computer that performs a Fourier transform, applies a digital filter, and performs an inverse Fourier transform. A reference beam and an object beam are focused by the focusing lens at a focal plane of the digital recorder to form a spatially low-frequency heterodyne hologram including spatially heterodyne fringes for Fourier analysis which is recorded by the digital recorder, and the computer transforms the recorded spatially low-frequency heterodyne hologram including spatially heterodyne fringes and shifts axes in Fourier space to sit on top of a heterodyne carrier frequency defined by an angle between the reference beam and the object beam and cuts off signals around an original origin before performing the inverse Fourier transform.

  11. Measurement of three-dimensional deformations using digital holography with radial sensitivity.

    PubMed

    Kohler, Christian; Viotti, Matias R; Albertazzi, G Armando

    2010-07-10

    A measurement system based on digital holography for the simultaneous measurement of out-of-plane and radial in-plane displacement fields for the assessment of residual stress is presented. Two holograms are recorded at the same time with a single image taken by a digital camera, allowing the separate evaluation of in-plane and out-of-plane movement. An axis-symmetrical diffractive optical element is used for the illumination of the object, which causes radial sensitivity vectors. By the addition and, respectively, the subtraction, of the four phase maps calculated from two camera frames, the in-plane and out-of-plane deformation of an object can be calculated separately. The device presented is suitable for high-speed, high-resolution measurement of residual stress. In addition to the setup, first measurement results and a short comparison to a mature digital speckle pattern interferometry setup are shown. PMID:20648180

  12. Content-based fused off-axis object illumination direct-to-digital holography

    DOEpatents

    Price, Jeffery R.

    2006-05-02

    Systems and methods are described for content-based fused off-axis illumination direct-to-digital holography. A method includes calculating an illumination angle with respect to an optical axis defined by a focusing lens as a function of data representing a Fourier analyzed spatially heterodyne hologram; reflecting a reference beam from a reference mirror at a non-normal angle; reflecting an object beam from an object the object beam incident upon the object at the illumination angle; focusing the reference beam and the object beam at a focal plane of a digital recorder to from the content-based off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis; and digitally recording the content based off-axis illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis.

  13. Perspectives of multimode fibers and digital holography for optogenetics

    NASA Astrophysics Data System (ADS)

    Czarske, Jürgen W.; Haufe, Daniel; Koukourakis, Nektarios; Büttner, Lars

    2016-04-01

    Optogenetic approaches allow the activation or inhibition of genetically prescribed populations of neurons by light. In principle, optogenetics offers not only the ability to elucidate the functions of neural circuitry, but also new approaches to a treatment of neurodegenerative diseases and recovery of vision and auditory perception. Optogenetics already has revolutionized research in neuroscience. However, new methods for delivering light to three-dimensionally distributed structures e.g. in the brain are necessary. A major hurdle for focusing light through biological tissue is the occurring scattering and scrambling of the light. We demonstrate the correction of the scrambling in a multimode fiber by digital optical phase conjugation with a perspective for optogenetics.

  14. Estimation of vibration frequency of loudspeaker diaphragm by parallel phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Kakue, T.; Endo, Y.; Shimobaba, T.; Ito, T.

    2014-11-01

    We report frequency estimation of loudspeaker diaphragm vibrating at high speed by parallel phase-shifting digital holography which is a technique of single-shot phase-shifting interferometry. This technique records multiple phaseshifted holograms required for phase-shifting interferometry by using space-division multiplexing. We constructed a parallel phase-shifting digital holography system consisting of a high-speed polarization-imaging camera. This camera has a micro-polarizer array which selects four linear polarization axes for 2 × 2 pixels. We set a loudspeaker as an object, and recorded vibration of diaphragm of the loudspeaker by the constructed system. By the constructed system, we demonstrated observation of vibration displacement of loudspeaker diaphragm. In this paper, we aim to estimate vibration frequency of the loudspeaker diaphragm by applying the experimental results to frequency analysis. Holograms consisting of 128 × 128 pixels were recorded at a frame rate of 262,500 frames per second by the camera. A sinusoidal wave was input to the loudspeaker via a phone connector. We observed displacement of the loudspeaker diaphragm vibrating by the system. We also succeeded in estimating vibration frequency of the loudspeaker diaphragm by applying frequency analysis to the experimental results.

  15. The suppression of phase error by applying window functions to digital holography

    NASA Astrophysics Data System (ADS)

    Yan, Facai; Yan, Hao; Yu, Yingjie; Zhou, Wenjing; Asundi, Anand

    2016-11-01

    Digital holography (DH) is a 3D imaging technique with a theoretical axial accuracy of around 1-2 nm. However, in practice, the axial error is generally quoted as tens of nanometers. Previous studies on sources of axial error mainly focused on the phase error introduced by lens. However, it was later shown that other factors such as the limited CCD aperture size also contribute to axial error. Based on this study, further investigation approaches to suppress the axial error caused by the limited CCD aperture size is discussed in this paper. Use of a window function to modify the shape of the hologram aperture after the recording process is proposed to reduce the axial error. The mechanism of how this window function reduces axial/phase error is analyzed. Specific features of this window function related to the axial error, namely the side lobe energy to main lobe energy ratio (SMER), is postulated. Both simulation and experiment are performed to validate that the selection of an appropriate window function helps to reduce the axial error of digital holography and SMER is an effective indicator in selection of an appropriate window function.

  16. Multiwavelength laser designed for single-frame digital holography.

    PubMed

    Tjörnhammar, Staffan; Eklöf, Finn Klemming; Yu, Zhangwei; Khodadad, Davood; Hällstig, Emil; Sjödahl, Mikael; Laurell, Fredrik

    2016-09-20

    In this paper, we present a tailored multiwavelength Yb-fiber laser source in the 1.03 μm spectral region for spatially multiplexed digital holographic acquisitions. The wavelengths with bandwidths below 0.1 nm were spectrally separated by approximately 1 nm by employing fiber Bragg gratings for spectral control. As a proof of concept, the shape of a cylindrically shaped object with a diameter of 48 mm was measured. The holographic acquisition was performed in single-shot dual-wavelength mode with a synthetic wavelength of 1.1 mm, and the accuracy was estimated to be 3% of the synthetic wavelength. PMID:27661577

  17. Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography.

    PubMed

    Mölder, A; Sebesta, M; Gustafsson, M; Gisselson, L; Wingren, A Gjörloff; Alm, K

    2008-11-01

    Manual cell counting is time consuming and requires a high degree of skill on behalf of the person performing the count. Here we use a technique that utilizes digital holography, allowing label-free and completely non-invasive cell counting directly in cell culture vessels with adherent viable cells. The images produced can provide both quantitative and qualitative phase information from a single hologram. The recently constructed microscope Holomonitor (Phase Holographic Imaging AB, Lund, Sweden) combines the commonly used phase contrast microscope with digital holography, the latter giving us the possibility of achieving quantitative information on cellular shape, area, confluence and optical thickness. This project aimed at determining the accuracy and repeatability of cell counting measurements using digital holography compared to the conventional manual cell counting method using a haemocytometer. The collected data were also used to determine cell size and cellular optical thickness. The results show that digital holography can be used for non-invasive automatic cell counting as precisely as conventional manual cell counting. PMID:19017223

  18. Remote monitoring of building oscillation modes by means of real-time Mid Infrared Digital Holography

    NASA Astrophysics Data System (ADS)

    Poggi, Pasquale; Locatelli, Massimiliano; Pugliese, Eugenio; Delle Donne, Dario; Lacanna, Giorgio; Meucci, Riccardo; Ripepe, Maurizio

    2016-04-01

    Non-destructive measurements of deformations are a quite common application of holography but due to the intrinsic limits in the interferometric technique, those are generally confined only to small targets and in controlled environment. Here we present an advanced technique, based on Mid Infrared Digital Holography (MIR DH), which works in outdoor conditions and provides remote and real-time information on the oscillation modes of large engineering structures. Thanks to the long wavelength of the laser radiation, large areas of buildings can be simultaneously mapped with sub-micrometric resolution in terms of their amplitude and frequency oscillation modes providing all the modal parameters vital for all the correct prevention strategies when the functionality and the health status of the structures have to be evaluated. The existing experimental techniques used to evaluate the fundamental modes of a structure are based either on seismometric sensors or on Ground-based Synthetic Aperture Radar (GbSAR). Such devices have both serious drawbacks, which prevent their application at a large scale or in the short term. We here demonstrate that the MIR DH based technique can fully overcome these limitations and has the potential to represent a breakthrough advance in the field of dynamic characterization of large structures.

  19. Remote monitoring of building oscillation modes by means of real-time Mid Infrared Digital Holography.

    PubMed

    Poggi, Pasquale; Locatelli, Massimiliano; Pugliese, Eugenio; Delle Donne, Dario; Lacanna, Giorgio; Meucci, Riccardo; Ripepe, Maurizio

    2016-01-01

    Non-destructive measurements of deformations are a quite common application of holography but due to the intrinsic limits in the interferometric technique, those are generally confined only to small targets and in controlled environment. Here we present an advanced technique, based on Mid Infrared Digital Holography (MIR DH), which works in outdoor conditions and provides remote and real-time information on the oscillation modes of large engineering structures. Thanks to the long wavelength of the laser radiation, large areas of buildings can be simultaneously mapped with sub-micrometric resolution in terms of their amplitude and frequency oscillation modes providing all the modal parameters vital for all the correct prevention strategies when the functionality and the health status of the structures have to be evaluated. The existing experimental techniques used to evaluate the fundamental modes of a structure are based either on seismometric sensors or on Ground-based Synthetic Aperture Radar (GbSAR). Such devices have both serious drawbacks, which prevent their application at a large scale or in the short term. We here demonstrate that the MIR DH based technique can fully overcome these limitations and has the potential to represent a breakthrough advance in the field of dynamic characterization of large structures. PMID:27032810

  20. Remote monitoring of building oscillation modes by means of real-time Mid Infrared Digital Holography

    PubMed Central

    Poggi, Pasquale; Locatelli, Massimiliano; Pugliese, Eugenio; Delle Donne, Dario; Lacanna, Giorgio; Meucci, Riccardo; Ripepe, Maurizio

    2016-01-01

    Non-destructive measurements of deformations are a quite common application of holography but due to the intrinsic limits in the interferometric technique, those are generally confined only to small targets and in controlled environment. Here we present an advanced technique, based on Mid Infrared Digital Holography (MIR DH), which works in outdoor conditions and provides remote and real-time information on the oscillation modes of large engineering structures. Thanks to the long wavelength of the laser radiation, large areas of buildings can be simultaneously mapped with sub-micrometric resolution in terms of their amplitude and frequency oscillation modes providing all the modal parameters vital for all the correct prevention strategies when the functionality and the health status of the structures have to be evaluated. The existing experimental techniques used to evaluate the fundamental modes of a structure are based either on seismometric sensors or on Ground-based Synthetic Aperture Radar (GbSAR). Such devices have both serious drawbacks, which prevent their application at a large scale or in the short term. We here demonstrate that the MIR DH based technique can fully overcome these limitations and has the potential to represent a breakthrough advance in the field of dynamic characterization of large structures. PMID:27032810

  1. Research on the methods of optical image hiding based on double random phase encoding and digital holography

    NASA Astrophysics Data System (ADS)

    Xu, Hongsheng; Sang, Nong

    2011-12-01

    Optical information hiding system has many features such as high processing speed, high parallel, high encryption dimension and high speed of optical transformation and related operations, more advantages than digital method in some way. But it has not adequate security, and enough combination with techniques of digital image processing. So on basis of analyzing existing image hiding and analyzing techniques, we give out the idea. We should adopt idea of virtual optics on the way of all-digital simulation to do research of optical image hiding and analyzing methods based on optical image processing technique especially technique of double random phase encoding and digital holography.

  2. Micro-light-pipe array with an excitation attenuation filter for lensless digital enzyme-linked immunosorbent assay

    NASA Astrophysics Data System (ADS)

    Takehara, Hironari; Nagasaki, Mizuki; Sasagawa, Kiyotaka; Takehara, Hiroaki; Noda, Toshihiko; Tokuda, Takashi; Ohta, Jun

    2016-03-01

    Digital enzyme-linked immunosorbent assay (ELISA) is used for detecting various biomarkers with hypersensitivity. We have been developing compact systems by replacing the fluorescence microscope with a CMOS image sensor. Here, we propose a micro-light-pipe array structure made of metal filled with dye-doped resin, which can be used as a fabrication substrate of the micro-reaction-chamber array of digital ELISA. The possibility that this structure enhances the coupling efficiency for fluorescence was simulated using a simple model. To realize the structure, we fabricated a 30-µm-thick micropipe array by copper electroplating around a thick photoresist pattern. The typical diameter of each fabricated micropipe was 10 µm. The pipes were filled with yellow-dye-doped epoxy resin. The transmittance ratio of fluorescence and excitation light could be controlled by adjusting the doping concentration. We confirmed that an angled excitation light incidence suppressed the leakage of excitation light.

  3. Dual-wavelength digital holography with a single low-coherence light source.

    PubMed

    Jeon, Sungbin; Cho, Janghyun; Jin, Ji-Nan; Park, No-Cheol; Park, Young-Pil

    2016-08-01

    We propose a measurement system using dual-wavelength digital holography and low-coherence interferometry to measure micro- and nanostructure surface heights. To achieve an extended axial step-measurement range and better image quality, a single light-emitting diode generates two distinct light sources by filtering different center wavelengths and narrower bandwidths. The system can measure surface profile with higher step heights and lower speckle noise in a large field-of-view. Using single-source lighting and a simple configuration, the method supports compactly configured and lower-cost surface-topography measurement systems applicable in various fields. Experimental results for a standard step sample verify the system's performance. PMID:27505804

  4. Evaporating droplet hologram simulation for digital in-line holography setup with divergent beam.

    PubMed

    Méès, Loïc; Grosjean, Nathalie; Chareyron, Delphine; Marié, Jean-Louis; Seifi, Mozhdeh; Fournier, Corinne

    2013-10-01

    Generalized Lorenz-Mie theory (GLMT) for a multilayered sphere is used to simulate holograms produced by evaporating spherical droplets with refractive index gradient in the surrounding air/vapor mixture. Simulated holograms provide a physical interpretation of experimental holograms produced by evaporating Diethyl Ether droplets with diameter in the order of 50 μm and recorded in a digital in-line holography configuration with a divergent beam. Refractive index gradients in the surrounding medium lead to a modification of the center part of the droplet holograms, where the first fringe is unusually bright. GLMT simulations reproduce this modification well, assuming an exponential decay of the refractive index from the droplet surface to infinity. The diverging beam effect is also considered. In both evaporating and nonevaporating cases, an equivalence is found between Gaussian beam and plane wave illuminations, simply based on a magnification ratio to be applied to the droplets' parameters.

  5. Design of a digital holography system for PFC erosion measurements on Proto-MPEX

    SciTech Connect

    ThomasJr., C. E.; Biewer, Theodore M; Baylor, Larry R; Combs, Stephen Kirk; Meitner, Steven J; Rapp, Juergen; Hillis, Donald Lee; Granstedt, E. M.; Majeski, R.; Kaita, R.

    2016-01-01

    A project has been started at ORNL to develop a dual-wavelength digital holography system for plasma facing component (PFC) erosion measurements on Proto-MPEX. Such a system will allow in-situ real-time measurements of component erosion. Initially the system will be developed with one laser, and first experimental laboratory measurements will be made with the single laser system. In the second year of development a second CO2 laser will be added and measurements with the dual wavelength system will begin. Adding the second wavelength allows measurements at a much longer synthetic wavelength equal to the average of the two wavelengths times the average divided by the difference of the two wavelengths.

  6. Large object investigation by digital holography with effective spectrum multiplexing under single-exposure approach

    SciTech Connect

    Liu, Ning Zhang, Yingying; Xie, Jun

    2014-10-13

    We present a method to investigate large object by digital holography with effective spectrum multiplexing under single-exposure approach. This method splits the original reference beam and redirects one of its branches as a second object beam. Through the modified Mach-Zehnder interferometer, the two object beams can illuminate different parts of the large object and create a spectrum multiplexed hologram onto the focal plane array of the charge-coupled device/complementary metal oxide semiconductor camera. After correct spectrum extraction and image reconstruction, the large object can be fully observed within only one single snap-shot. The flexibility and great performance make our method a very attractive and promising technique for large object investigation under common 632.8 nm illumination.

  7. Hologram encoding strategies for non-Bayesian noise suppression in digital holography reconstructions and optical display

    NASA Astrophysics Data System (ADS)

    Bianco, V.; Memmolo, P.; Finizio, A.; Paturzo, M.; Ferraro, P.

    2016-03-01

    Here we first propose a fast, one-shot, non-Bayesian method which performs a numerical synthesis of a moving aperture in order to reduce the noise in Digital Holography without prior information on its statistics. Starting from one single hologram capture, multiple uncorrelated reconstructions are provided by random sparse resampling masks, which can be incoherently averaged. Thus, the problem of the setup complexity introduced by multiple recordings gets solved. Besides, at the scope of performing DH display using a SLM, it is highly required to operate directly on the hologram, in order to obtain its denoised version without losing the coherence between amplitude and phase information. We then move a step forward, showing a novel encoding formula allowing us to directly synthesize denoised holograms to be optically displayed by SLMs.

  8. Sparsity-Based Pixel Super Resolution for Lens-Free Digital In-line Holography

    PubMed Central

    Song, Jun; Leon Swisher, Christine; Im, Hyungsoon; Jeong, Sangmoo; Pathania, Divya; Iwamoto, Yoshiko; Pivovarov, Misha; Weissleder, Ralph; Lee, Hakho

    2016-01-01

    Lens-free digital in-line holography (LDIH) is a promising technology for portable, wide field-of-view imaging. Its resolution, however, is limited by the inherent pixel size of an imaging device. Here we present a new computational approach to achieve sub-pixel resolution for LDIH. The developed method is a sparsity-based reconstruction with the capability to handle the non-linear nature of LDIH. We systematically characterized the algorithm through simulation and LDIH imaging studies. The method achieved the spatial resolution down to one-third of the pixel size, while requiring only single-frame imaging without any hardware modifications. This new approach can be used as a general framework to enhance the resolution in nonlinear holographic systems. PMID:27098438

  9. Acquisition and replay systems for direct-to-digital holography and holovision

    DOEpatents

    Thomas, Clarence E.; Hanson, Gregory R.

    2003-02-25

    Improvements to the acquisition and replay systems for direct-to-digital holography and holovision are described. A method of recording an off-axis hologram includes: splitting a laser beam into an object beam and a reference beam; reflecting the reference beam from a reference beam mirror; reflecting the object beam from an illumination beamsplitter; passing the object beam through an objective lens; reflecting the object beam from an object; focusing the reference beam and the object beam at a focal plane of a digital recorder to form an off-axis hologram; digitally recording the off-axis hologram; and transforming the off-axis hologram in accordance with a Fourier transform to obtain a set of results. A method of writing an off-axis hologram includes: passing a laser beam through a spatial light modulator; and focusing the laser beam at a focal plane of a photorefractive crystal to impose a holographic diffraction grating pattern on the photorefractive crystal. A method of replaying an off-axis hologram includes: illuminating a photorefractive crystal having a holographic diffraction grating with a replay beam.

  10. Comparative analysis of autofocus functions in digital in-line phase-shifting holography.

    PubMed

    Fonseca, Elsa S R; Fiadeiro, Paulo T; Pereira, Manuela; Pinheiro, António

    2016-09-20

    Numerical reconstruction of digital holograms relies on a precise knowledge of the original object position. However, there are a number of relevant applications where this parameter is not known in advance and an efficient autofocusing method is required. This paper addresses the problem of finding optimal focusing methods for use in reconstruction of digital holograms of macroscopic amplitude and phase objects, using digital in-line phase-shifting holography in transmission mode. Fifteen autofocus measures, including spatial-, spectral-, and sparsity-based methods, were evaluated for both synthetic and experimental holograms. The Fresnel transform and the angular spectrum reconstruction methods were compared. Evaluation criteria included unimodality, accuracy, resolution, and computational cost. Autofocusing under angular spectrum propagation tends to perform better with respect to accuracy and unimodality criteria. Phase objects are, generally, more difficult to focus than amplitude objects. The normalized variance, the standard correlation, and the Tenenbaum gradient are the most reliable spatial-based metrics, combining computational efficiency with good accuracy and resolution. A good trade-off between focus performance and computational cost was found for the Fresnelet sparsity method. PMID:27661596

  11. Comparative analysis of autofocus functions in digital in-line phase-shifting holography.

    PubMed

    Fonseca, Elsa S R; Fiadeiro, Paulo T; Pereira, Manuela; Pinheiro, António

    2016-09-20

    Numerical reconstruction of digital holograms relies on a precise knowledge of the original object position. However, there are a number of relevant applications where this parameter is not known in advance and an efficient autofocusing method is required. This paper addresses the problem of finding optimal focusing methods for use in reconstruction of digital holograms of macroscopic amplitude and phase objects, using digital in-line phase-shifting holography in transmission mode. Fifteen autofocus measures, including spatial-, spectral-, and sparsity-based methods, were evaluated for both synthetic and experimental holograms. The Fresnel transform and the angular spectrum reconstruction methods were compared. Evaluation criteria included unimodality, accuracy, resolution, and computational cost. Autofocusing under angular spectrum propagation tends to perform better with respect to accuracy and unimodality criteria. Phase objects are, generally, more difficult to focus than amplitude objects. The normalized variance, the standard correlation, and the Tenenbaum gradient are the most reliable spatial-based metrics, combining computational efficiency with good accuracy and resolution. A good trade-off between focus performance and computational cost was found for the Fresnelet sparsity method.

  12. Fused off-axis object illumination direct-to-digital holography with a plurality of illumination sources

    DOEpatents

    Price, Jeffery R.; Bingham, Philip R.

    2005-11-08

    Systems and methods are described for rapid acquisition of fused off-axis illumination direct-to-digital holography. A method of recording a plurality of off-axis object illuminated spatially heterodyne holograms, each of the off-axis object illuminated spatially heterodyne holograms including spatially heterodyne fringes for Fourier analysis, includes digitally recording, with a first illumination source of an interferometer, a first off-axis object illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis; and digitally recording, with a second illumination source of the interferometer, a second off-axis object illuminated spatially heterodyne hologram including spatially heterodyne fringes for Fourier analysis.

  13. The influence of hologram aperture on speckle noise in the reconstructed image of digital holography and its reduction

    NASA Astrophysics Data System (ADS)

    Cai, Xiao-ou; Wang, Hui

    2008-01-01

    Based on the whole process of the recording and reconstruction of digital holography, we study the formation cause of speckle noise in its reconstructed image and acquire the conclusion that the small size of hologram aperture diffraction aggravates the speckle noise of reconstructed image and the speckle noise has been one of primary noise sources in the reconstruction process. In order to reduce the speckle noise resulting from little hologram aperture diffraction, we set an appropriate aperture function matching the recording parameter and aperture size of hologram and deconvolve the reconstructed image with it. The validity has been proved in theory and experiment. Therefore, it offers a brand-new thought and practical way to reduce the speckle noise in the reconstructed image of digital holography.

  14. Simultaneous amplitude-contrast and phase-contrast surface plasmon resonance imaging by use of digital holography

    PubMed Central

    Li, Shiping; Zhong, Jingang

    2012-01-01

    The surface plasmon resonance imaging technique provides a tool that allows high-throughput analysis and real-time kinetic measurement. A simultaneous amplitude-contrast and phase-contrast surface plasmon resonance imaging method is presented. The amplitude-contrast and phase-contrast images are simultaneously obtained by use of digital holography. The detection sensitivity of amplitude-contrast imaging and phase-contrast imaging can compensate for each other. Thus, the detectable sample components may cover a wider range of refractive index values for the simultaneous amplitude-contrast and phase-contrast imaging method than for the phase-contrast imaging method or amplitude-contrast imaging method. A detailed description of the theory and an experiment of monitoring the evaporation process of a drop of NaCl injection in real time are presented. In addition, the amplitude-contrast image has less coherent noise by digital holography. PMID:23243569

  15. Evaluating unsupervised methods to size and classify suspended particles using digital in-line holography

    USGS Publications Warehouse

    Davies, Emlyn J.; Buscombe, Daniel D.; Graham, George W.; Nimmo-Smith, W. Alex M.

    2015-01-01

    Substantial information can be gained from digital in-line holography of marine particles, eliminating depth-of-field and focusing errors associated with standard lens-based imaging methods. However, for the technique to reach its full potential in oceanographic research, fully unsupervised (automated) methods are required for focusing, segmentation, sizing and classification of particles. These computational challenges are the subject of this paper, in which we draw upon data collected using a variety of holographic systems developed at Plymouth University, UK, from a significant range of particle types, sizes and shapes. A new method for noise reduction in reconstructed planes is found to be successful in aiding particle segmentation and sizing. The performance of an automated routine for deriving particle characteristics (and subsequent size distributions) is evaluated against equivalent size metrics obtained by a trained operative measuring grain axes on screen. The unsupervised method is found to be reliable, despite some errors resulting from over-segmentation of particles. A simple unsupervised particle classification system is developed, and is capable of successfully differentiating sand grains, bubbles and diatoms from within the surf-zone. Avoiding miscounting bubbles and biological particles as sand grains enables more accurate estimates of sand concentrations, and is especially important in deployments of particle monitoring instrumentation in aerated water. Perhaps the greatest potential for further development in the computational aspects of particle holography is in the area of unsupervised particle classification. The simple method proposed here provides a foundation upon which further development could lead to reliable identification of more complex particle populations, such as those containing phytoplankton, zooplankton, flocculated cohesive sediments and oil droplets.

  16. Focus detection in digital holography by cross-sectional images of propagating waves

    NASA Astrophysics Data System (ADS)

    Özcan, Meriç

    2014-02-01

    In digital holography, computing a focused image of an object requires a prior knowledge of the distance of the object from the camera. When this distance is not known, it is necessary to repeat the image reconstruction at a range of distances followed by evaluation of each image with a sharpness metric to determine the in-focus distance of the object. Here, we present a method to nd the focus distance by processing the image transverse to the object plane instead of the processing in the image plane as it is usually done. Since the reconstructed hologram image is spatially symmetric around the focus point along the propagation axis, simply nding the symmetry points in the image cross-section speci es the focus location, and no other sharpness metrics are necessary to use. Also with this method, it is possible to nd the focus distances of multiple objects simultaneously, including the phase only objects without any staining. We will present the simulations and the experimental results obtained by a digital holographic microscope.

  17. Parametric studies of adaptive optics by self-interference incoherent digital holography

    NASA Astrophysics Data System (ADS)

    Hong, Jisoo; Kim, Myung K.

    2014-02-01

    Adaptive optics (AO) in astronomical imaging is a technique to improve the quality of image by compensating aberrations induced by atmospheric turbulence. Digital holographic AO (DHAO) is one attractive option to implement AO scheme because it is capable of directly measuring the phase profile of aberration without complicated calculation or loss of resolution of CCD. Hence, if applicable, DHAO is expected to have advantages over traditional AO systems. Recent development of self-interference incoherent digital holography (SIDH) makes it possible to apply the concept of DHAO for an astronomical application where the illumination is incoherent and cannot be controlled. We have investigated the image characteristics according to various parameters of SIDH AO to derive optimum condition or design of the system. We observe not only well-known super-resolution property of SIDH but also interesting and significant improvement of noise behavior by aberration compensation. Because of many beneficial features, we expect that SIDH AO will be a useful tool for astronomical imaging.

  18. Holographic storage system based on digital holography for recording a phase data page in a compact optical setup

    NASA Astrophysics Data System (ADS)

    Nobukawa, Teruyoshi; Nomura, Takanori

    2016-03-01

    A holographic storage system based on digital holography is proposed for recording and retrieving a phase data page in a compact and simple optical setup. In the proposed recording system, complex amplitude distribution can be modulated using a single phase-only spatial light modulator. The complex amplitude distribution of a retrieved phase data page is detected with the Fourier fringe analysis. The use of digital holographic techniques enables realizing a compact and simple holographic recording system, which is independent of misalignment problem in conventional holographic storage systems. The capability of the proposed recording system is numerically and experimentally evaluated.

  19. Phase-shifting digital holography with a low-coherence light source for reconstruction of a digital relief object hidden behind a light-scattering medium.

    PubMed

    Tamano, Shingo; Hayasaki, Yoshio; Nishida, Nobuo

    2006-02-10

    Hiding image data with a light-scattering medium is effective as a basic data protection technique. The hidden image data can be observed only by using a low-coherence interference technique and is thus protected from unauthorized access. Unlike an intensity-distributed object, a digital relief object has no intensity distribution, making it possible to hide its existence by using a dilute light-scattering medium. To reconstruct the digital relief object through the light-scattering medium, we developed phase-shifting digital holography with a low-coherence light source. The experimental performance, including the spatial resolution and phase error of the reconstructed image, is estimated.

  20. Comparison of three-dimensional particle tracking and sizing using plenoptic imaging and digital in-line holography.

    PubMed

    Hall, Elise M; Thurow, Brian S; Guildenbecher, Daniel R

    2016-08-10

    Digital in-line holography (DIH) and plenoptic photography are two techniques for single-shot, volumetric measurement of 3D particle fields. Here we present a comparison of the two methods by applying plenoptic imaging to experimental configurations that have been previously investigated with DIH. These experiments include the tracking of secondary droplets from the impact of a water drop on a thin film of water and tracking of pellets from a shotgun. Both plenoptic imaging and DIH successfully quantify the 3D nature of these particle fields. This includes measurement of the 3D particle position, individual particle sizes, and three-component velocity vectors. For the initial processing methods presented here, both techniques give out-of-plane positional accuracy of approximately 1-2 particle diameters. For a fixed image sensor, digital holography achieves higher effective in-plane spatial resolutions. However, collimated and coherent illumination makes holography susceptible to image distortion through index of refraction gradients, as demonstrated in the shotgun experiments. In contrast, plenoptic imaging allows for a simpler experimental configuration and, due to the use of diffuse, white-light illumination, plenoptic imaging is less susceptible to image distortion in the shotgun experiments.

  1. Comparison of three-dimensional particle tracking and sizing using plenoptic imaging and digital in-line holography.

    PubMed

    Hall, Elise M; Thurow, Brian S; Guildenbecher, Daniel R

    2016-08-10

    Digital in-line holography (DIH) and plenoptic photography are two techniques for single-shot, volumetric measurement of 3D particle fields. Here we present a comparison of the two methods by applying plenoptic imaging to experimental configurations that have been previously investigated with DIH. These experiments include the tracking of secondary droplets from the impact of a water drop on a thin film of water and tracking of pellets from a shotgun. Both plenoptic imaging and DIH successfully quantify the 3D nature of these particle fields. This includes measurement of the 3D particle position, individual particle sizes, and three-component velocity vectors. For the initial processing methods presented here, both techniques give out-of-plane positional accuracy of approximately 1-2 particle diameters. For a fixed image sensor, digital holography achieves higher effective in-plane spatial resolutions. However, collimated and coherent illumination makes holography susceptible to image distortion through index of refraction gradients, as demonstrated in the shotgun experiments. In contrast, plenoptic imaging allows for a simpler experimental configuration and, due to the use of diffuse, white-light illumination, plenoptic imaging is less susceptible to image distortion in the shotgun experiments. PMID:27534487

  2. Novel Perspectives on the Characterization of Species-Dependent Optical Signatures of Bacterial Colonies by Digital Holography

    PubMed Central

    Buzalewicz, Igor; Kujawińska, Małgorzata; Krauze, Wojciech; Podbielska, Halina

    2016-01-01

    The use of light diffraction for the microbiological diagnosis of bacterial colonies was a significant breakthrough with widespread implications for the food industry and clinical practice. We previously confirmed that optical sensors for bacterial colony light diffraction can be used for bacterial identification. This paper is focused on the novel perspectives of this method based on digital in-line holography (DIH), which is able to reconstruct the amplitude and phase properties of examined objects, as well as the amplitude and phase patterns of the optical field scattered/diffracted by the bacterial colony in any chosen observation plane behind the object from single digital hologram. Analysis of the amplitude and phase patterns inside a colony revealed its unique optical properties, which are associated with the internal structure and geometry of the bacterial colony. Moreover, on a computational level, it is possible to select the desired scattered/diffracted pattern within the entire observation volume that exhibits the largest amount of unique, differentiating bacterial features. These properties distinguish this method from the already proposed sensing techniques based on light diffraction/scattering of bacterial colonies. The reconstructed diffraction patterns have a similar spatial distribution as the recorded Fresnel patterns, previously applied for bacterial identification with over 98% accuracy, but they are characterized by both intensity and phase distributions. Our results using digital holography provide new optical discriminators of bacterial species revealed in one single step in form of new optical signatures of bacterial colonies: digital holograms, reconstructed amplitude and phase patterns, as well as diffraction patterns from all observation space, which exhibit species-dependent features. To the best of our knowledge, this is the first report on bacterial colony analysis via digital holography and our study represents an innovative approach

  3. Novel Perspectives on the Characterization of Species-Dependent Optical Signatures of Bacterial Colonies by Digital Holography.

    PubMed

    Buzalewicz, Igor; Kujawińska, Małgorzata; Krauze, Wojciech; Podbielska, Halina

    2016-01-01

    The use of light diffraction for the microbiological diagnosis of bacterial colonies was a significant breakthrough with widespread implications for the food industry and clinical practice. We previously confirmed that optical sensors for bacterial colony light diffraction can be used for bacterial identification. This paper is focused on the novel perspectives of this method based on digital in-line holography (DIH), which is able to reconstruct the amplitude and phase properties of examined objects, as well as the amplitude and phase patterns of the optical field scattered/diffracted by the bacterial colony in any chosen observation plane behind the object from single digital hologram. Analysis of the amplitude and phase patterns inside a colony revealed its unique optical properties, which are associated with the internal structure and geometry of the bacterial colony. Moreover, on a computational level, it is possible to select the desired scattered/diffracted pattern within the entire observation volume that exhibits the largest amount of unique, differentiating bacterial features. These properties distinguish this method from the already proposed sensing techniques based on light diffraction/scattering of bacterial colonies. The reconstructed diffraction patterns have a similar spatial distribution as the recorded Fresnel patterns, previously applied for bacterial identification with over 98% accuracy, but they are characterized by both intensity and phase distributions. Our results using digital holography provide new optical discriminators of bacterial species revealed in one single step in form of new optical signatures of bacterial colonies: digital holograms, reconstructed amplitude and phase patterns, as well as diffraction patterns from all observation space, which exhibit species-dependent features. To the best of our knowledge, this is the first report on bacterial colony analysis via digital holography and our study represents an innovative approach

  4. Real-time terahertz digital holography with a quantum cascade laser

    PubMed Central

    Locatelli, Massimiliano; Ravaro, Marco; Bartalini, Saverio; Consolino, Luigi; Vitiello, Miriam S.; Cicchi, Riccardo; Pavone, Francesco; De Natale, Paolo

    2015-01-01

    Coherent imaging in the THz range promises to exploit the peculiar capabilities of these wavelengths to penetrate common materials like plastics, ceramics, paper or clothes with potential breakthroughs in non-destructive inspection and quality control, homeland security and biomedical applications. Up to now, however, THz coherent imaging has been limited by time-consuming raster scanning, point-like detection schemes and by the lack of adequate coherent sources. Here, we demonstrate real-time digital holography (DH) at THz frequencies exploiting the high spectral purity and the mW output power of a quantum cascade laser combined with the high sensitivity and resolution of a microbolometric array. We show that, in a one-shot exposure, phase and amplitude information of whole samples, either in reflection or in transmission, can be recorded. Furthermore, a 200 times reduced sensitivity to mechanical vibrations and a significantly enlarged field of view are observed, as compared to DH in the visible range. These properties of THz DH enable unprecedented holographic recording of real world dynamic scenes. PMID:26315647

  5. Real-time terahertz digital holography with a quantum cascade laser.

    PubMed

    Locatelli, Massimiliano; Ravaro, Marco; Bartalini, Saverio; Consolino, Luigi; Vitiello, Miriam S; Cicchi, Riccardo; Pavone, Francesco; De Natale, Paolo

    2015-01-01

    Coherent imaging in the THz range promises to exploit the peculiar capabilities of these wavelengths to penetrate common materials like plastics, ceramics, paper or clothes with potential breakthroughs in non-destructive inspection and quality control, homeland security and biomedical applications. Up to now, however, THz coherent imaging has been limited by time-consuming raster scanning, point-like detection schemes and by the lack of adequate coherent sources. Here, we demonstrate real-time digital holography (DH) at THz frequencies exploiting the high spectral purity and the mW output power of a quantum cascade laser combined with the high sensitivity and resolution of a microbolometric array. We show that, in a one-shot exposure, phase and amplitude information of whole samples, either in reflection or in transmission, can be recorded. Furthermore, a 200 times reduced sensitivity to mechanical vibrations and a significantly enlarged field of view are observed, as compared to DH in the visible range. These properties of THz DH enable unprecedented holographic recording of real world dynamic scenes. PMID:26315647

  6. Real-time terahertz digital holography with a quantum cascade laser

    NASA Astrophysics Data System (ADS)

    Locatelli, Massimiliano; Ravaro, Marco; Bartalini, Saverio; Consolino, Luigi; Vitiello, Miriam S.; Cicchi, Riccardo; Pavone, Francesco; de Natale, Paolo

    2015-08-01

    Coherent imaging in the THz range promises to exploit the peculiar capabilities of these wavelengths to penetrate common materials like plastics, ceramics, paper or clothes with potential breakthroughs in non-destructive inspection and quality control, homeland security and biomedical applications. Up to now, however, THz coherent imaging has been limited by time-consuming raster scanning, point-like detection schemes and by the lack of adequate coherent sources. Here, we demonstrate real-time digital holography (DH) at THz frequencies exploiting the high spectral purity and the mW output power of a quantum cascade laser combined with the high sensitivity and resolution of a microbolometric array. We show that, in a one-shot exposure, phase and amplitude information of whole samples, either in reflection or in transmission, can be recorded. Furthermore, a 200 times reduced sensitivity to mechanical vibrations and a significantly enlarged field of view are observed, as compared to DH in the visible range. These properties of THz DH enable unprecedented holographic recording of real world dynamic scenes.

  7. Ultrafast axial scanning for two-photon microscopy via a digital micromirror device and binary holography.

    PubMed

    Cheng, Jiyi; Gu, Chenglin; Zhang, Dapeng; Wang, Dien; Chen, Shih-Chi

    2016-04-01

    In this Letter, we present an ultrafast nonmechanical axial scanning method for two-photon excitation (TPE) microscopy based on binary holography using a digital micromirror device (DMD), achieving a scanning rate of 4.2 kHz, scanning range of ∼180  μm, and scanning resolution (minimum step size) of ∼270  nm. Axial scanning is achieved by projecting the femtosecond laser to a DMD programmed with binary holograms of spherical wavefronts of increasing/decreasing radii. To guide the scanner design, we have derived the parametric relationships between the DMD parameters (i.e., aperture and pixel size), and the axial scanning characteristics, including (1) maximum optical power, (2) minimum step size, and (3) scan range. To verify the results, the DMD scanner is integrated with a custom-built TPE microscope that operates at 60 frames per second. In the experiment, we scanned a pollen sample via both the DMD scanner and a precision z-stage. The results show the DMD scanner generates images of equal quality throughout the scanning range. The overall efficiency of the TPE system was measured to be ∼3%. With the high scanning rate, the DMD scanner may find important applications in random-access imaging or high-speed volumetric imaging that enables visualization of highly dynamic biological processes in 3D with submillisecond temporal resolution. PMID:27192259

  8. Direct-to-digital holography reduction of reference hologram noise and fourier space smearing

    DOEpatents

    Voelkl, Edgar

    2006-06-27

    Systems and methods are described for reduction of reference hologram noise and reduction of Fourier space smearing, especially in the context of direct-to-digital holography (off-axis interferometry). A method of reducing reference hologram noise includes: recording a plurality of reference holograms; processing the plurality of reference holograms into a corresponding plurality of reference image waves; and transforming the corresponding plurality of reference image waves into a reduced noise reference image wave. A method of reducing smearing in Fourier space includes: recording a plurality of reference holograms; processing the plurality of reference holograms into a corresponding plurality of reference complex image waves; transforming the corresponding plurality of reference image waves into a reduced noise reference complex image wave; recording a hologram of an object; processing the hologram of the object into an object complex image wave; and dividing the complex image wave of the object by the reduced noise reference complex image wave to obtain a reduced smearing object complex image wave.

  9. Terahertz in-line digital holography of human hepatocellular carcinoma tissue

    PubMed Central

    Rong, Lu; Latychevskaia, Tatiana; Chen, Chunhai; Wang, Dayong; Yu, Zhengping; Zhou, Xun; Li, Zeyu; Huang, Haochong; Wang, Yunxin; Zhou, Zhou

    2015-01-01

    Terahertz waves provide a better contrast in imaging soft biomedical tissues than X-rays, and unlike X-rays, they cause no ionisation damage, making them a good option for biomedical imaging. Terahertz absorption imaging has conventionally been used for cancer diagnosis. However, the absorption properties of a cancerous sample are influenced by two opposing factors: an increase in absorption due to a higher degree of hydration and a decrease in absorption due to structural changes. It is therefore difficult to diagnose cancer from an absorption image. Phase imaging can thus be critical for diagnostics. We demonstrate imaging of the absorption and phase-shift distributions of 3.2 mm × 2.3 mm × 30-μm-thick human hepatocellular carcinoma tissue by continuous-wave terahertz digital in-line holography. The acquisition time of a few seconds for a single in-line hologram is much shorter than that of other terahertz diagnostic techniques, and future detectors will allow acquisition of meaningful holograms without sample dehydration. The resolution of the reconstructions was enhanced by sub-pixel shifting and extrapolation. Another advantage of this technique is its relaxed minimal sample size limitation. The fibrosis indicated in the phase distribution demonstrates the potential of terahertz holographic imaging to obtain a more objective, early diagnosis of cancer. PMID:25676705

  10. Dynamic DIC by digital holography microscopy for enhancing phase-contrast visualization.

    PubMed

    Miccio, Lisa; Finizio, Andrea; Puglisi, Roberto; Balduzzi, Donatella; Galli, Andrea; Ferraro, Pietro

    2011-01-13

    Differential image contrast (DIC), through the numerical managing and manipulation of complex wavefronts obtained by digital holography (DH), is investigated. We name the approach Dynamical Differential Holographic Image Contrast (DDHIC). DDHIC dispenses from special optics and/or complex setup configurations with moveable components, as usually occurs in classical DIC, that is not well-suited for investigating objects experiencing dynamic evolution during the measurement. In fact, the technique presented here, is useful for floating samples since it allows, from a single recording, to set a posteriori the best conditions for DIC imaging in conjunction with the numerical focusing feature of DH. By DDHIC, the movies can be easily built-up to offering dynamic representation of phase-contrast along all directions, thus improving the visualization. Furthermore, the dynamic representation is useful for making the proper choice of other key parameters of DIC such as the amount of shear and the bias, with the aim to optimize the visualized phase-contrast imaging as favorite representation for bio-scientists. Investigation is performed on various biological samples.

  11. Simple method for direct determination of bending strains by use of digital holography.

    PubMed

    Hung, M Y; Lin, L; Shang, H M

    2001-09-01

    A computer-based technique is described for direct determination of bending strains in beam and plate structures. First a displacement-related phase-change map is constructed by digital holography and computer-vision techniques. Subsequently the computer generates an exact replica of the phase-change map, then overlays the two identical maps and, finally, rotates one of the maps through 180 degrees relative to the other in their planes about a point of interest. The local curvatures and the local twist of the bent surface at the point of interest are determined from the conic sections that are reconstructed from the algebraic sum of the phase changes at the vicinity of this point, thus permitting further calculation for determination of the local bending strains. When the need arises, bending moments and stresses may be determined concurrently. As the optical setup is simple, with computer-based data acquisition and processing, the entire system is user friendly, and rapid measurement is achieved.

  12. Calibration of particle position on digital holography using transparent resin block with dispersed particles

    NASA Astrophysics Data System (ADS)

    Tanaka, Y.; Yoshino, T.; Harada, D.; Murata, S.

    2009-11-01

    This paper describes the use of a Calibration Block (CB) for evaluating the accuracy of digital holography in particle position measurement. CB made of acrylic has three layers and the gap between the layers is filled with transparent resin. The refractive index of the resin and the layers is almost the same (1.49). Fin Block (FB), which is not filled with resin, is introduced in order to evaluate effects of the resin. The fringe edges of several holographic patterns are observed by using three kinds of CB and FB. Each layer is coated with spherical particles (diameter: 16.36 ± 0.42 μ m). The influence of multiple scattering on the detected depth of the particles is evaluated by changing the number density of particles. Three kinds of CB and FB are prepared (averaged particle density in the holographic pattern; 83.2, 166.5 and 249.7). The fringe edges of the holographic patterns generated in CB and FB are observed, respectively. It is found that the fringe edges of CB are clearer than FB. Also, Multiple scattering acts as a source of background noise with high spatial frequency, which has almost the same frequency as that of the particle diffraction on the fringe patterns, and reduces the effective signal-to-noise ratio of the holographic pattern. CB can be used to evaluate the influence of multiple scattering on the detected particle depth.

  13. High-speed femtosecond laser beam shaping based on binary holography using a digital micromirror device.

    PubMed

    Cheng, Jiyi; Gu, Chenglin; Zhang, Dapeng; Chen, Shih-Chi

    2015-11-01

    In this Letter, we present a digital micromirror device (DMD)-based ultrafast beam shaper, i.e., DUBS. To our knowledge, the DUBS is the first binary laser beam shaper that can generate high-resolution (1140×912 pixels) arbitrary beam modes for femtosecond lasers at a rate of 4.2 kHz; the resolution and pattern rate are limited by the DMD. In the DUBS, the spectrum of the input pulsed laser is first angularly dispersed by a transmission grating and subsequently imaged to a DMD with beam modulation patterns; the transmission grating and a high-reflectivity mirror together compensate the angular dispersion introduced by the DMD. The mode of the output beam is monitored by a CCD camera. In the experiments, the DUBS is programmed to generate four different beam modes, including an Airy beam, Bessel beam, Laguerre-Gaussian (LG) beam, and a custom-designed "peace-dove" beam via the principle of binary holography. To verify the high shaping rate, the Airy beam and LG beam are generated alternately at 4.2 kHz, i.e., the maximum pattern rate of our DMD. The overall efficiency of the DUBS is measured to be 4.7%. With the high-speed and high-resolution beam-shaping capability, the DUBS may find important applications in nonlinear microscopy, optical manipulation, and microscale/nanoscale laser machining, etc.

  14. Digital holography reconstruction algorithms to estimate the morphology and depth of nonspherical absorbing particles

    NASA Astrophysics Data System (ADS)

    Guildenbecher, Daniel R.; Gao, Jian; Reu, Phillip L.; Chen, Jun

    2012-09-01

    In digital holography an object wave is numerically reconstructed from a recorded hologram. Using this technique it is possible to detect the position and size of particles in a 3D domain. In this work, particular focus is placed on quantification of particles with non-spherical morphologies. The in-line configuration is chosen due to the simplicity of the optical setup and minimal distortions of in-plane morphologies. However, this geometry is also characterized by a large depth-of-focus and high uncertainty in the detected depth. To quantify these uncertainties, this work begins with the definition of a non-dimensional model of hologram recording and reconstruction applied to single spherical and nonspherical particles. Typical CCD noise sources are included. Application of this model to two particle detection methods reveals the relevant merits and limitations of each particle detection method. From the lessons learned, a new hybrid particle detection method is proposed. Simulations indicate the hybrid method significantly improves upon the accuracy of the measured depth and particle morphologies. Furthermore, the proposed method automatically determines the optimum threshold for each particle, and, therefore, requires minimal user inputs. Finally, initial experimental results for spherical particles confirm the accuracy of the proposed hybrid method.

  15. Lensless reflective point diffraction interferometer.

    PubMed

    Zhu, Wenhua; Chen, Lei; Zheng, Donghui; Yang, Ying; Han, Zhigang; Li, Jinpeng

    2016-07-01

    A lensless reflective point diffraction interferometer (LRPDI) is proposed for dynamic wavefront measurement. The point diffraction interferometer is integrated on a small substrate with properly designed thin film, which is used for generating the interferogram with high carrier frequency at a CCD target. By lensless imaging, the complex amplitude at the CCD target can be propagated to the conjugated plane of the exit pupil of an incident wavefront, which not only avoids the edge diffraction in the interferogram, but also eliminates systematic error. The accuracy of LRPDI is demonstrated by simulation and experiment, and a precision better than 1/150 wavelength is achieved. The new design with lensless imaging processing is suitable for dynamic wavefront measurement. PMID:27409204

  16. Tile-Based Two-Dimensional Phase Unwrapping for Digital Holography Using a Modular Framework.

    PubMed

    Antonopoulos, Georgios C; Steltner, Benjamin; Heisterkamp, Alexander; Ripken, Tammo; Meyer, Heiko

    2015-01-01

    A variety of physical and biomedical imaging techniques, such as digital holography, interferometric synthetic aperture radar (InSAR), or magnetic resonance imaging (MRI) enable measurement of the phase of a physical quantity additionally to its amplitude. However, the phase can commonly only be measured modulo 2π, as a so called wrapped phase map. Phase unwrapping is the process of obtaining the underlying physical phase map from the wrapped phase. Tile-based phase unwrapping algorithms operate by first tessellating the phase map, then unwrapping individual tiles, and finally merging them to a continuous phase map. They can be implemented computationally efficiently and are robust to noise. However, they are prone to failure in the presence of phase residues or erroneous unwraps of single tiles. We tried to overcome these shortcomings by creating novel tile unwrapping and merging algorithms as well as creating a framework that allows to combine them in modular fashion. To increase the robustness of the tile unwrapping step, we implemented a model-based algorithm that makes efficient use of linear algebra to unwrap individual tiles. Furthermore, we adapted an established pixel-based unwrapping algorithm to create a quality guided tile merger. These original algorithms as well as previously existing ones were implemented in a modular phase unwrapping C++ framework. By examining different combinations of unwrapping and merging algorithms we compared our method to existing approaches. We could show that the appropriate choice of unwrapping and merging algorithms can significantly improve the unwrapped result in the presence of phase residues and noise. Beyond that, our modular framework allows for efficient design and test of new tile-based phase unwrapping algorithms. The software developed in this study is freely available.

  17. Tile-Based Two-Dimensional Phase Unwrapping for Digital Holography Using a Modular Framework.

    PubMed

    Antonopoulos, Georgios C; Steltner, Benjamin; Heisterkamp, Alexander; Ripken, Tammo; Meyer, Heiko

    2015-01-01

    A variety of physical and biomedical imaging techniques, such as digital holography, interferometric synthetic aperture radar (InSAR), or magnetic resonance imaging (MRI) enable measurement of the phase of a physical quantity additionally to its amplitude. However, the phase can commonly only be measured modulo 2π, as a so called wrapped phase map. Phase unwrapping is the process of obtaining the underlying physical phase map from the wrapped phase. Tile-based phase unwrapping algorithms operate by first tessellating the phase map, then unwrapping individual tiles, and finally merging them to a continuous phase map. They can be implemented computationally efficiently and are robust to noise. However, they are prone to failure in the presence of phase residues or erroneous unwraps of single tiles. We tried to overcome these shortcomings by creating novel tile unwrapping and merging algorithms as well as creating a framework that allows to combine them in modular fashion. To increase the robustness of the tile unwrapping step, we implemented a model-based algorithm that makes efficient use of linear algebra to unwrap individual tiles. Furthermore, we adapted an established pixel-based unwrapping algorithm to create a quality guided tile merger. These original algorithms as well as previously existing ones were implemented in a modular phase unwrapping C++ framework. By examining different combinations of unwrapping and merging algorithms we compared our method to existing approaches. We could show that the appropriate choice of unwrapping and merging algorithms can significantly improve the unwrapped result in the presence of phase residues and noise. Beyond that, our modular framework allows for efficient design and test of new tile-based phase unwrapping algorithms. The software developed in this study is freely available. PMID:26599984

  18. Tile-Based Two-Dimensional Phase Unwrapping for Digital Holography Using a Modular Framework

    PubMed Central

    Antonopoulos, Georgios C.; Steltner, Benjamin; Heisterkamp, Alexander; Ripken, Tammo; Meyer, Heiko

    2015-01-01

    A variety of physical and biomedical imaging techniques, such as digital holography, interferometric synthetic aperture radar (InSAR), or magnetic resonance imaging (MRI) enable measurement of the phase of a physical quantity additionally to its amplitude. However, the phase can commonly only be measured modulo 2π, as a so called wrapped phase map. Phase unwrapping is the process of obtaining the underlying physical phase map from the wrapped phase. Tile-based phase unwrapping algorithms operate by first tessellating the phase map, then unwrapping individual tiles, and finally merging them to a continuous phase map. They can be implemented computationally efficiently and are robust to noise. However, they are prone to failure in the presence of phase residues or erroneous unwraps of single tiles. We tried to overcome these shortcomings by creating novel tile unwrapping and merging algorithms as well as creating a framework that allows to combine them in modular fashion. To increase the robustness of the tile unwrapping step, we implemented a model-based algorithm that makes efficient use of linear algebra to unwrap individual tiles. Furthermore, we adapted an established pixel-based unwrapping algorithm to create a quality guided tile merger. These original algorithms as well as previously existing ones were implemented in a modular phase unwrapping C++ framework. By examining different combinations of unwrapping and merging algorithms we compared our method to existing approaches. We could show that the appropriate choice of unwrapping and merging algorithms can significantly improve the unwrapped result in the presence of phase residues and noise. Beyond that, our modular framework allows for efficient design and test of new tile-based phase unwrapping algorithms. The software developed in this study is freely available. PMID:26599984

  19. Singlet oxygen detection in water by means of digital holography and digital holographic tomography

    NASA Astrophysics Data System (ADS)

    Belashov, A. V.; Petrov, N. V.; Semenova, I. V.; Vasyutinskii, O. S.

    2016-04-01

    The paper presents results on singlet oxygen detection in aqueous solutions of a photosensitizer based on the reconstruction of 3D temperature gradients resulting from nonradiative deactivation of excited oxygen molecules. 3D temperature distributions were reconstructed by means of the inverse Abel transformation from a single digital hologram in the case of cylindrically symmetric distribution of the temperature gradient and using holographic tomography algorithm with filtered back projection in the case of nonsymmetrical distribution. Major features of the applied techniques are discussed and results obtained by the two methods are compared.

  20. Differential-interference-contrast digital in-line holography microscopy based on a single-optical-element.

    PubMed

    Zhang, Yuchao; Xie, Changqing

    2015-11-01

    Both digital in-line holography (DIH) and zone plate-based microscopy have received considerable interest as powerful imaging tools. However, the former suffers from a twin-image noise problem. The latter suffers from low efficiency and difficulty in fabrication. Here, we present an effective and efficient phase-contrast imaging approach, named differential-interference-contrast digital in-line holography (DIC-DIH), by using a single optical element to split the incident light into a plane wave and a converging spherical wave and generate a two-dimensional (2D) DIC effect simultaneously. Specifically, to improve image contrast, we present a new single optical element, termed 2D DIC compound photon sieves, by combining two overlaid binary gratings and a compound photon sieve through two logical XOR operations. The proof-of-concept experiments demonstrate that the proposed technique can eliminate the twin-image noise problem and improve image contrast with high efficiency. Additionally, we present an example of the phase-contrast imaging nonuniform thick photoresist development process.

  1. Hard X-ray Fourier Transform Holography Using a Reference Scatterer Fabricated by Electron-Beam-Assisted Chemical-Vapor Deposition

    SciTech Connect

    Suzuki, M.; Isogami, S.; Tsunoda, M.; Takahashi, S.; Ishio, S.

    2011-09-09

    We present a fabrication method for a reference source that is efficient when used for lensless Fourier transform holography. This method produces a reference source that yields high spatial resolution and enhanced signal-to-noise ratio in a Fourier-transformed real-space image, and is particularly useful for Fourier transform holography experiments in the hard x-ray region.

  2. Femtosecond digital in-line holography with the fractional Fourier transform: application to phase-contrast metrology

    NASA Astrophysics Data System (ADS)

    Brunel, M.; Shen, H.; Coetmellec, S.; Lebrun, D.; Ait Ameur, K.

    2012-03-01

    The possibility to analyze transparent phase objects using femtosecond digital in-line holography is demonstrated. We show that the diffraction patterns produced by the diffraction of 20-fs pulses by pure phase objects can be advantageously processed by using the fractional Fourier transform. The optimal fractional orders lead to the longitudinal location of the phase object while the analysis of the reconstructed patterns leads to its diameter and to the value of the phase shift. Simulations and experimental results are in good concordance. This work shows new important applications of these reconstruction techniques in the domain of digital phase-contrast metrology. We further show that the use of femtosecond pulses results in a significant reduction of coherent noise.

  3. Improvement of the size estimation of 3D tracked droplets using digital in-line holography with joint estimation reconstruction

    NASA Astrophysics Data System (ADS)

    Verrier, N.; Grosjean, N.; Dib, E.; Méès, L.; Fournier, C.; Marié, J.-L.

    2016-04-01

    Digital holography is a valuable tool for three-dimensional information extraction. Among existing configurations, the originally proposed set-up (i.e. Gabor, or in-line holography), is reasonably immune to variations in the experimental environment making it a method of choice for studies of fluid dynamics. Nevertheless, standard hologram reconstruction techniques, based on numerical light back-propagation are prone to artifacts such as twin images or aliases that limit both the quality and quantity of information extracted from the acquired holograms. To get round this issue, the hologram reconstruction as a parametric inverse problem has been shown to accurately estimate 3D positions and the size of seeding particles directly from the hologram. To push the bounds of accuracy on size estimation still further, we propose to fully exploit the information redundancy of a hologram video sequence using joint estimation reconstruction. Applying this approach in a bench-top experiment, we show that it led to a relative precision of 0.13% (for a 60 μm diameter droplet) for droplet size estimation, and a tracking precision of {σx}× {σy}× {σz}=0.15× 0.15× 1~\\text{pixels} .

  4. Single-shot dual-wavelength phase reconstruction in off-axis digital holography with polarization-multiplexing transmission.

    PubMed

    Wang, Zhe; Jiang, Zhuqing; Chen, Yifei

    2016-08-01

    A new system for single-shot dual-wavelength digital holographic microscopy with polarization-multiplexing path-shared transmission is presented. The key feature of the optical configuration is that the interference waves of two wavelengths having orthogonal polarization can transmit in the same interferometer paths at the same time, and two polarizers orthogonal to each other are placed in front of the CCD to realize single-shot recording of two holograms. The correlative filtering algorithm of the spatial-frequency spectrum for dual-wavelength digital holograms is reliable and efficient in the dual-wavelength path-shared configuration. The phase reconstruction in dual-wavelength digital holographic imaging is achieved by using this filtering algorithm. The experiment results of phase reconstruction of a groove grating demonstrate the reliability and validity of this optical configuration and the correlative filtering algorithm. This polarization-multiplexing configuration for dual-wavelength digital holography is compact and has more flexibility for the replacement of different-wavelength lasers. PMID:27505390

  5. Quantitative, three-dimensional imaging of aluminum drop combustion in solid propellant plumes via digital in-line holography.

    PubMed

    Guildenbecher, Daniel R; Cooper, Marcia A; Gill, Walter; Stauffacher, Howard Lee; Oliver, Michael S; Grasser, Thomas W

    2014-09-01

    Burning aluminized propellants eject reacting molten aluminum drops with a broad size distribution. Prior to this work, in situ measurement of the drop size statistics and other quantitative flow properties was complicated by the narrow depth-of-focus of microscopic videography. Here, digital in-line holography (DIH) is demonstrated for quantitative volumetric imaging of the propellant plume. For the first time, to the best of our knowledge, in-focus features, including burning surfaces, drop morphologies, and reaction zones, are automatically measured through a depth spanning many millimeters. By quantifying all drops within the line of sight, DIH provides an order of magnitude increase in the effective data rate compared to traditional imaging. This enables rapid quantification of the drop size distribution with limited experimental repetition.

  6. Wavefront holoscopy: application of digital in-line holography for the inspection of engraved marks in progressive addition lenses.

    PubMed

    Perucho, Beatriz; Micó, Vicente

    2014-01-01

    Progressive addition lenses (PALs) are engraved with permanent marks at standardized locations in order to guarantee correct centering and alignment throughout the manufacturing and mounting processes. Out of the production line, engraved marks provide useful information about the PAL as well as act as locator marks to re-ink again the removable marks. Even though those marks should be visible by simple visual inspection with the naked eye, engraving marks are often faint and weak, obscured by scratches, and partially occluded and difficult to recognize on tinted or antireflection-coated lenses. Here, we present an extremely simple optical device (named as wavefront holoscope) for visualization and characterization of permanent marks in PAL based on digital in-line holography. Essentially, a point source of coherent light illuminates the engraved mark placed just before a CCD camera that records a classical Gabor in-line hologram. The recorded hologram is then digitally processed to provide a set of high-contrast images of the engraved marks. Experimental results are presented showing the applicability of the proposed method as a new ophthalmic instrument for visualization and characterization of engraved marks in PALs. PMID:24449144

  7. Noise and signal scaling factors in digital holography in weak illumination: relationship with shot noise.

    PubMed

    Lesaffre, M; Verrier, N; Gross, M

    2013-01-01

    We have performed off-axis heterodyne holography with very weak illumination by recording holograms of the object with and without object illumination in the same acquisition run. We have experimentally studied how the reconstructed image signal (with illumination) and noise background (without) scale with the holographic acquisition and reconstruction parameters that are the number of frames and the number of pixels of the reconstruction spatial filter. The first parameter is related to the frequency bandwidth of detection in time, the second one to the bandwidth in space. The signal to background ratio varies roughly like the inverse of the bandwidth in time and space. We have also compared the noise background with the theoretical shot-noise background calculated by Monte Carlo simulation. The experimental and Monte Carlo noise background agree very well with each other.

  8. Three-dimensional velocity near-wall measurements by digital in-line holography: calibration and results.

    PubMed

    Allano, Daniel; Malek, Mokrane; Walle, Françoise; Corbin, Frédéric; Godard, Gilles; Coëtmellec, Sébastien; Lecordier, Bertrand; Foucaut, Jean-Marc; Lebrun, Denis

    2013-01-01

    Velocity measurements in the vicinity of an obstacle remain very complicated even when optical diagnostics based on displacement of micrometric tracers are considered. In the present paper, digital in-line holography with a divergent beam is proposed to measure the three-dimensional (3D) velocity vector fields in a turbulent boundary layer and, in particular, on the near wall region of a wind tunnel. The seeding droplets (1-5 μm) transported by a turbulent airflow are illuminated by a couple of laser pulses coming from a fiber coupled laser diode. These double exposure holograms are then recorded through a transparent glass reticle specially designed for this application with an accurate surface positioning combined with a particularly attractive in situ calibration method of the investigation volume (less than 10 mm(3)). The method used for processing holograms recorded in such a configuration is detailed. Our original calibration procedure and the assessment of its accuracy are presented. Our holographic probe has been tested in a wind tunnel for a large range of different velocities. Then 3D velocity vector fields extracted from more than 13000 holograms are analyzed. Statistical results show the capability of our approach to access in a turbulent boundary layer. In particular, it leads to relevant measurements for fluid mechanics such as velocity fluctuation and the shear stress in the very close vicinity of a wall. PMID:23292426

  9. Spatially-resolved, three-dimensional spray characterization of impinging jets by digital in-line holography

    NASA Astrophysics Data System (ADS)

    Gao, Jian; Rodrigues, Neil; Sojka, Paul; Chen, Jun

    2014-11-01

    The impinging jet injector is a preferred method for the atomization of liquid rocket propellants. The majority of experimental studies in literature are not spatially-resolved due to the limitations of widely available point-wise and two-dimensional (2D) diagnostic techniques such as phase Doppler anemometry (PDA), which requires significant experimental repetitions to give spatially-resolved measurements. In the present study, digital in-line holography (DIH) is used to provide spatially-resolved, three-dimensional (3D) characteristics of impinging jet sprays. A double-exposure DIH setup is configured to measure droplet 3D, three-component velocity as well as the size distribution. The particle information is extracted by the hybrid method, which is recently proposed as a particle detection method. To enlarge the detection volume, two parallel, collimated laser beams are used to simultaneously probe the spray at two locations, and two identical cameras are used to record the corresponding holograms. Such a setup has a detection volume of approximately 20 cm by 3.6 cm by 4.8 cm. Sprays of both Newtonian and non-Newtonian liquids corresponding to regimes at relatively lower jet Reynolds and Weber numbers are investigated. Measurements from DIH are further verified by comparison with experimental data obtained from shadowgraph and PDA. It is revealed that DIH is particularly suitable to provide spatially-resolved, 3D measurements of impinging jet sprays that are not particularly dense.

  10. Digital in-line holography: 4-D imaging and tracking of micro-structures and organisms in microfluidics and biology

    NASA Astrophysics Data System (ADS)

    Garcia-Sucerquia, J.; Xu, W.; Jericho, S. K.; Jericho, M. H.; Tamblyn, I.; Kreuzer, H. J.

    2006-01-01

    In recent years, in-line holography as originally proposed by Gabor, supplemented with numerical reconstruction, has been perfected to the point at which wavelength resolution both laterally and in depth is routinely achieved with light by using digital in-line holographic microscopy (DIHM). The advantages of DIHM are: (1) simplicity of the hardware (laser- pinhole-CCD camera), (2) magnification is obtained in the numerical reconstruction, (3) maximum information of the 3-D structure with a depth of field of millimeters, (4) changes in the specimen and the simultaneous motion of many species, can be followed in 4-D at the camera frame rate. We present results obtained with DIHM in biological and microfluidic applications. By taking advantage of the large depth of field and the plane-to-plane reconstruction capability of DIHM, we can produce 3D representations of the paths followed by micron-sized objects such as suspensions of microspheres and biological samples (cells, algae, protozoa, bacteria). Examples from biology include a study of the motion of bacteria in a diatom and the track of algae and paramecium. In microfluidic applications we observe micro-channel flow, motion of bubbles in water and evolution in electrolysis. The paper finishes with new results from an underwater version of DIHM.

  11. Ultra-fast digital holography of the femto-second order

    NASA Astrophysics Data System (ADS)

    Zhai, Hongchen; Wang, Xiaolei; Mu, Guoguang

    2007-01-01

    We report on pulsed digital micro holographic systems recording ultra-fast process of the femto-second order, by spatially angular division multiplexing (SADM) and wavelength division multiplexing (WDM), respectively. Both intensity and phase images of the digitally reconstructed images are obtained through Fourier transformation and digital filtering, which show clearly the plasma forming and propagating dynamic process of laser induced ionization of ambient air at the wavelength of 800 nm, with a time resolution of 50 fs and frame intervals of 300 to 550 fs.

  12. Pulsed digital holography system recording ultrafast process of the femtosecond order

    NASA Astrophysics Data System (ADS)

    Wang, Xiaolei; Zhai, Hongchen; Mu, Guoguang

    2006-06-01

    We report, for the first time to our knowledge, a pulsed digital microholographic system with spatial angular multiplexing for recording the ultrafast process of the femtosecond order. The optimized design of the two sets of subpulse-train generators in this system makes it possible to implement a digital holographic recording with spatial angular multiplexing of a frame interval of the femtosecond order, while keeping the incident angle of the object beams unchanged. Three pairs of amplitude and phase images from the same view angle digitally reconstructed by the system demonstrated the ultrafast dynamic process of laser-induced ionization of ambient air at a wavelength of 800 nm, with a time resolution of 50 fs and a frame interval of 300 fs.

  13. Multi-wavelengths digital holography: reconstruction, synthesis and display of holograms using adaptive transformation.

    PubMed

    Memmolo, P; Finizio, A; Paturzo, M; Ferraro, P; Javidi, B

    2012-05-01

    A method based on spatial transformations of multiwavelength digital holograms and the correlation matching of their numerical reconstructions is proposed, with the aim to improve superimposition of different color reconstructed images. This method is based on an adaptive affine transform of the hologram that permits management of the physical parameters of numerical reconstruction. In addition, we present a procedure to synthesize a single digital hologram in which three different colors are multiplexed. The optical reconstruction of the synthetic hologram by a spatial light modulator at one wavelength allows us to display all color features of the object, avoiding loss of details.

  14. Two-stage method to suppress speckle noise in digital holography

    NASA Astrophysics Data System (ADS)

    Leng, Junmin; Zhou, Jinhe; Lang, Xiaoping; Li, Xiaoying

    2015-10-01

    The two-stage method is proposed to suppress speckle noise in the digital hologram. Three kinds of optical denoising ways are analyzed and compared at first. The optimal one is used to reduce speckle preliminarily. At the same time, the statistical property of the speckle is changed by the optical way. Then the optimized NLM algorithm is adopted to further suppress speckle noise. The experimental system is set up, and the performance indices are calculated. The results are compared with other algorithms. It is demonstrated that the presented method can effectively suppress speckle noise in the digital hologram and the processed image is very vivid.

  15. Intrinsic spatial shift of local focus metric curves in digital inline holography for accurate 3D morphology measurement of irregular micro-objects

    NASA Astrophysics Data System (ADS)

    Wu, Yingchun; Wu, Xuecheng; Lebrun, Denis; Brunel, Marc; Coëtmellec, Sébastien; Lesouhaitier, Olivier; Chen, Jia; Gréhan, Gérard

    2016-09-01

    A theoretical model of digital inline holography system reveals that the local focus metric curves (FMCs) of different parts of an irregular micro-object present spatial shift in the depth direction which is resulted from the depth shift. Thus, the 3D morphology of an irregular micro-object can be accurately measured using the cross correlation of the local FMCs. This method retrieves the 3D depth information directly, avoiding the uncertainty inherited from the depth position determination. Typical 3D morphology measurements, including the 3D boundary lines of tilted carbon fibers and irregular coal particles, and the 3D swimming gesture of a live Caenorhabdities elegans, are presented.

  16. Lensless in-line holographic microscope with Talbot grating illumination.

    PubMed

    Feng, Shaodong; Wang, Mingjun; Wu, Jigang

    2016-07-15

    We have developed a wide field-of-view lensless in-line holographic microscope (LIHM) capable of acquiring microscopic images with a compact design. In our imaging system, a Ronchi grating was illuminated by a collimated laser beam to generate a Talbot self-imaging grating illumination on the sample, and the in-line holograms were recorded by a CMOS imaging sensor behind the sample. An iterative reconstruction algorithm was then applied to reconstruct the sample image while eliminating the twin-image background that appears in traditional in-line holography. In the algorithm, the dark areas of the illumination grating were used as a known constraint to define the sample support that led to convergence of the iteration. The whole-sample image can be acquired by laterally shifting the grating. We demonstrated the performance of our iteration algorithm and imaging system by successfully acquiring images of polystyrene microspheres with 5 μm diameter and the wing of a green lacewing. PMID:27420484

  17. Research on object-plane constraints and hologram expansion in phase retrieval algorithms for continuous-wave terahertz inline digital holography reconstruction.

    PubMed

    Hu, Jiaqi; Li, Qi; Cui, Shanshan

    2014-10-20

    In terahertz inline digital holography, zero-order diffraction light and conjugate images can cause the reconstructed image to be blurred. In this paper, three phase retrieval algorithms are applied to conduct reconstruction based on the same near-field diffraction propagation conditions and image-plane constraints. The impact of different object-plane constraints on CW terahertz inline digital holographic reconstruction is studied. The results show that in the phase retrieval algorithm it is not suitable to impose restriction on the phase when the object is not isolated in the transmission-type CW terahertz inline digital holography. In addition, the effects of zero-padding expansion, boundary replication expansion, and apodization operation on reconstructed images are studied. The results indicate that the conjugate image can be eliminated, and a better reconstructed image can be obtained by adopting an appropriate phase retrieval algorithm after the normalized hologram extending to the minimum area, which meets the applicable range of the angular spectrum reconstruction algorithm by means of boundary replication.

  18. Dual-wavelength digital holography for 3D particle image velocimetry: experimental validation.

    PubMed

    Grare, S; Allano, D; Coëtmellec, S; Perret, G; Corbin, F; Brunel, M; Gréhan, G; Lebrun, D

    2016-01-20

    A multi-exposure digital in-line hologram of a particle field is recorded by two successive pulses of different wavelengths. During the reconstruction step, each recording can be independently analyzed by selecting a given wavelength. This procedure enables avoiding the superimposition of particle images that may be close to each other.

  19. Thin wetting film lensless imaging

    NASA Astrophysics Data System (ADS)

    Allier, C. P.; Poher, V.; Coutard, J. G.; Hiernard, G.; Dinten, J. M.

    2011-03-01

    Lensless imaging has recently attracted a lot of attention as a compact, easy-to-use method to image or detect biological objects like cells, but failed at detecting micron size objects like bacteria that often do not scatter enough light. In order to detect single bacterium, we have developed a method based on a thin wetting film that produces a micro-lens effect. Compared with previously reported results, a large improvement in signal to noise ratio is obtained due to the presence of a micro-lens on top of each bacterium. In these conditions, standard CMOS sensors are able to detect single bacterium, e.g. E.coli, Bacillus subtilis and Bacillus thuringiensis, with a large signal to noise ratio. This paper presents our sensor optimization to enhance the SNR; improve the detection of sub-micron objects; and increase the imaging FOV, from 4.3 mm2 to 12 mm2 to 24 mm2, which allows the detection of bacteria contained in 0.5μl to 4μl to 10μl, respectively.

  20. Single-shot digital holography for fast measuring optical properties of fibers.

    PubMed

    Agour, Mostafa; El-Farahaty, Keremal; Seisa, Eman; Omar, Emam; Sokkar, Taha

    2015-10-01

    We propose a fast method for measuring optical properties, e.g., the refractive index profile and birefringence, of fibers. It is based on recovering the phase distribution of light refracted by a fiber sample at the recording plane from a single-shot digital hologram. During the recovering process, an optimized approach based on the spatial carrier frequency method was utilized. The proposed approach enhances affects that arise from the limited spatial extent of the bandpass filter associated with the implementation of the spatial carrier frequency method. In contrast to the low spatial resolution of off-axis digital holograms, the method ensures the best utilization of the camera support. From the recovered phase information, the optical path difference is measured; thus, the refractive index profile, the mean refractive index, and the birefringence of isotactic polypropylene (IPP) are determined. Experimental results are given for illustration. PMID:26479652

  1. Experimental verification of phase retrieval of microbeads in high-speed phase imaging using digital holography

    NASA Astrophysics Data System (ADS)

    Matoba, Osamu; Xia, Peng; Quan, Xiangyu; Nagahama, Naoya; Tanimoto, Shunsuke; Nitta, Kouichi; Awatsuji, Yasuhiro

    2016-06-01

    One of fast measurement systems of μm-size phase objects based on digital holographic microscope with transmission geometry is presented. For building a 3D inspection system of the phase objects, the improvement of recovered phase image is discussed. Under the CW laser illumination, the movement afterimage of phase object was observed. The phase object is recovered by deconvolution filter. Experimental and numerical evaluation are presented.

  2. Parallel phase-shifting self-interference digital holography with faithful reconstruction using compressive sensing

    NASA Astrophysics Data System (ADS)

    Wan, Yuhong; Man, Tianlong; Wu, Fan; Kim, Myung K.; Wang, Dayong

    2016-11-01

    We present a new self-interference digital holographic approach that allows single-shot capturing three-dimensional intensity distribution of the spatially incoherent objects. The Fresnel incoherent correlation holographic microscopy is combined with parallel phase-shifting technique to instantaneously obtain spatially multiplexed phase-shifting holograms. The compressive-sensing-based reconstruction algorithm is implemented to reconstruct the original object from the under sampled demultiplexed holograms. The scheme is verified with simulations. The validity of the proposed method is experimentally demonstrated in an indirectly way by simulating the use of specific parallel phase-shifting recording device.

  3. Direct to Digital Holography for Semiconductor Wafer Defect Detection and Review

    SciTech Connect

    ThomasJr., C. E.; Bahm, Tracy M.; Baylor, Larry R; Bingham, Philip R.; Burns, Steven W.; Chidley, Matthew D; Dai, Xiaolong; Delahanty, Robert J.; Doti, Christopher J.; El-Khashab, Ayman; Fisher, Robert L.; Gilbert, Judd M.; Cui, Hongtao; Goddard Jr, James Samuel; Hanson, Gregory R; Hickson, Joel D.; Hunt, Martin A.; Hylton, Kathy W; John, George C.; Jones, Michael L.; McDonald, Kenneth R.; Mayo, Michael W.; McMackin, Ian; Patek, David; Price, John H.; Rasmussen, David A; Schaefer, Louis J.; Scheidt, Thomas R.; Schulze, Mark A.; Schumaker, Philip D.; Shen, Bichuan; Smith, Randall G.; Su, Allen N.; Tobin Jr, Kenneth William; Usry, William R.; Voelkl, Edgar; Weber, Karsten S.; Jones, Paul G.; Owen, Robert W.

    2002-01-01

    A method for recording true holograms (not holographic interferometry) directly to a digital video medium in a single image has been invented. This technology makes the amplitude and phase for every pixel of the target object wave available. Since phase is proportional to wavelength, this makes high-resolution metrology an implicit part of the holographic recording. Measurements of phase can be made to one hundredth or even one thousandth of a wavelength, so the technology is attractive for finding defects on semiconductor wafers, where feature sizes are now smaller than the wavelength of even deep ultra-violet light.

  4. Common-path depth-filtered digital holography for high resolution imaging of buried semiconductor structures

    NASA Astrophysics Data System (ADS)

    Finkeldey, Markus; Schellenberg, Falk; Gerhardt, Nils C.; Paar, Christof; Hofmann, Martin R.

    2016-03-01

    We investigate digital holographic microscopy (DHM) in reflection geometry for non-destructive 3D imaging of semiconductor devices. This technique provides high resolution information of the inner structure of a sample while maintaining its integrity. To illustrate the performance of the DHM, we use our setup to localize the precise spots for laser fault injection, in the security related field of side-channel attacks. While digital holographic microscopy techniques easily offer high resolution phase images of surface structures in reflection geometry, they are typically incapable to provide high quality phase images of buried structures due to the interference of reflected waves from different interfaces inside the structure. Our setup includes a sCMOS camera for image capture, arranged in a common-path interferometer to provide very high phase stability. As a proof of principle, we show sample images of the inner structure of a modern microcontroller. Finally, we compare our holographic method to classic optical beam induced current (OBIC) imaging to demonstrate its benefits.

  5. Improved particle size estimation in digital holography via sign matched filtering.

    PubMed

    Lu, Jiang; Shaw, Raymond A; Yang, Weidong

    2012-06-01

    A matched filter method is provided for obtaining improved particle size estimates from digital in-line holograms. This improvement is relative to conventional reconstruction and pixel counting methods for particle size estimation, which is greatly limited by the CCD camera pixel size. The proposed method is based on iterative application of a sign matched filter in the Fourier domain, with sign meaning the matched filter takes values of ±1 depending on the sign of the angular spectrum of the particle aperture function. Using simulated data the method is demonstrated to work for particle diameters several times the pixel size. Holograms of piezoelectrically generated water droplets taken in the laboratory show greatly improved particle size measurements. The method is robust to additive noise and can be applied to real holograms over a wide range of matched-filter particle sizes.

  6. Common path in-line holography using enhanced joint object reference digital interferometers

    PubMed Central

    Kelner, Roy; Katz, Barak; Rosen, Joseph

    2014-01-01

    Joint object reference digital interferometer (JORDI) is a recently developed system capable of recording holograms of various types [Opt. Lett. 22(5), 4719 (2013)]. Presented here is a new enhanced system design that is based on the previous JORDI. While the previous JORDI has been based purely on diffractive optical elements, displayed on spatial light modulators, the present design incorporates an additional refractive objective lens, thus enabling hologram recording with improved resolution and increased system applicability. Experimental results demonstrate successful hologram recording for various types of objects, including transmissive, reflective, three-dimensional, phase and highly scattering objects. The resolution limit of the system is analyzed and experimentally validated. Finally, the suitability of JORDI for microscopic applications is verified as a microscope objective based configuration of the system is demonstrated. PMID:24663838

  7. Reconstruction of refractive-index distribution in off-axis digital holography optical diffraction tomographic system.

    PubMed

    Kozacki, Tomasz; Krajewski, Rafal; Kujawińska, Małgorzata

    2009-08-01

    In the paper the optical diffraction tomographic system for reconstruction of the internal refractive index distribution in optical fiber utilizing grating Mach-Zehnder interferometer configuration is explored. The setup applies afocal imaging. Conventional grating application gives, however, highly aberrated object beam producing incorrect refractive-index reconstructions. The grating inherent aberrations are characterized, its influence on both image projections and refractive index reconstructions is presented. To remove aberrations and enable tomographic reconstruction a novel digital holographic algorithm, correcting optical system imaging, is developed. The algorithm uses plane wave spectrum decomposition of optical field for solving diffraction problem between parallel and tilted planes and enabling correction of imaging system aberrations. The algorithm concept was successfully proved in simulations and the experiment.

  8. Pulsed digital holography for high-speed contouring that uses a two-wavelength method.

    PubMed

    Pedrini, G; Fröning, P; Tiziani, H J; Gusev, M E

    1999-06-01

    A two-wavelength method for a fast shape measurement by use of a pulsed ruby laser is presented. The wavelength change is produced by alteration of the distance between the plates of the laser's output etalon. One plate of the etalon is mounted on a vibrating piezoelectric element; this allows a fast wavelength change. Two holograms at different wavelengths are recorded in a few microseconds by use of a CCD. The holograms are reconstructed digitally, and the wave-front phase is calculated. The shape is obtained by subtraction of the phases of the wave fronts recorded at different wavelengths. Environmental disturbances at low frequencies, such as air turbulence, vibrations, and object drift, have no influence on the measurement. Experimental results are presented. PMID:18319945

  9. High-speed digital holography for neutral gas and electron density imaging.

    PubMed

    Granstedt, E M; Thomas, C E; Kaita, R; Majeski, R; Baylor, L R; Meitner, S J; Combs, S K

    2016-05-01

    An instrument was developed using digital holographic reconstruction of the wavefront from a CO2 laser imaged on a high-speed commercial IR camera. An acousto-optic modulator is used to generate 1-25 μs pulses from a continuous-wave CO2 laser, both to limit the average power at the detector and also to freeze motion from sub-interframe time scales. Extensive effort was made to characterize and eliminate noise from vibrations and second-surface reflections. Mismatch of the reference and object beam curvature initially contributed substantially to vibrational noise, but was mitigated through careful positioning of identical imaging lenses. Vibrational mode amplitudes were successfully reduced to ≲1 nm for frequencies ≳50 Hz, and the inter-frame noise across the 128 × 128 pixel window which is typically used is ≲2.5 nm. To demonstrate the capabilities of the system, a piezo-electric valve and a reducing-expanding nozzle were used to generate a super-sonic gas jet which was imaged with high spatial resolution (better than 0.8 lp/mm) at high speed. Abel inversions were performed on the phase images to produce 2-D images of localized gas density. This system could also be used for high spatial and temporal resolution measurements of plasma electron density or surface deformations. PMID:27250423

  10. Dual-Utility of Digital Holography & Epi-Fluorescence to Localize Cellular Nuclei

    NASA Astrophysics Data System (ADS)

    Sheldrake, Eric

    Digital Holographic Microscopy (DHM) and Epi-Fluorescence have been combined for biological imaging. DHM is a non-invasive phase contrast microscopy technique that provides quantitative information such as the variations in refractive index and physical height. 3D physical height and refractive index profiles are imaged for HEK293 and CHO cells. These values and profiles are compared with known values for the sample gathered from literature and other microscopy techniques, including Scanning Electron Microscopy. The measured CHO cellular length is (15.31 +/- 2.60) microm. Localization of cellular nuclei is performed using the Epi-Fluorescence setup and the DNA specific fluorophore DAPI. The fluorescence intensity profile was imaged, where nuclei shape, size, and localization are compared using an A.1 Zeiss Fluorescence Microscope. The CHO cells are comparable with apoptotic cells, where the measured nucleus length is (10.91 +/- 2.27) microm. DHM and Epi-Fluorescence are combined to analyze the physical heights of the cell as well as localize its nucleus. The combination of these techniques are greatly advantageous to understand cellular functions and variability.

  11. A new 3D tracking method exploiting the capabilities of digital holography in microscopy

    NASA Astrophysics Data System (ADS)

    Miccio, L.; Memmolo, P.; Merola, F.; Fusco, S.; Embrione, V.; Netti, P. A.; Ferraro, P.

    2013-04-01

    A method for 3D tracking has been developed exploiting Digital Holographic Microscopy (DHM) features. In the framework of self-consistent platform for manipulation and measurement of biological specimen we use DHM for quantitative and completely label free analysis of specimen with low amplitude contrast. Tracking capability extend the potentiality of DHM allowing to monitor the motion of appropriate probes and correlate it with sample properties. Complete 3D tracking has been obtained for the probes avoiding the issue of amplitude refocusing in traditional tracking processing. Our technique belongs to the video tracking methods that, conversely from Quadrant Photo-Diode method, opens the possibility to track multiples probes. All the common used video tracking algorithms are based on the numerical analysis of amplitude images in the focus plane and the shift of the maxima in the image plane are measured after the application of an appropriate threshold. Our approach for video tracking uses different theoretical basis. A set of interferograms is recorded and the complex wavefields are managed numerically to obtain three dimensional displacements of the probes. The procedure works properly on an higher number of probes and independently from their size. This method overcomes the traditional video tracking issues as the inability to measure the axial movement and the choice of suitable threshold mask. The novel configuration allows 3D tracking of micro-particles and simultaneously can furnish Quantitative Phase-contrast maps of tracked micro-objects by interference microscopy, without changing the configuration. In this paper, we show a new concept for a compact interferometric microscope that can ensure the multifunctionality, accomplishing accurate 3D tracking and quantitative phase-contrast analysis. Experimental results are presented and discussed for in vitro cells. Through a very simple and compact optical arrangement we show how two different functionalities

  12. Improved non-invasive method for aerosol particle charge measurement employing in-line digital holography

    NASA Astrophysics Data System (ADS)

    Tripathi, Anjan Kumar

    Electrically charged particles are found in a wide range of applications ranging from electrostatic powder coating, mineral processing, and powder handling to rain-producing cloud formation in atmospheric turbulent flows. In turbulent flows, particle dynamics is influenced by the electric force due to particle charge generation. Quantifying particle charges in such systems will help in better predicting and controlling particle clustering, relative motion, collision, and growth. However, there is a lack of noninvasive techniques to measure particle charges. Recently, a non-invasive method for particle charge measurement using in-line Digital Holographic Particle Tracking Velocimetry (DHPTV) technique was developed in our lab, where charged particles to be measured were introduced to a uniform electric field, and their movement towards the oppositely charged electrode was deemed proportional to the amount of charge on the particles (Fan Yang, 2014 [1]). However, inherent speckle noise associated with reconstructed images was not adequately removed and therefore particle tracking data was contaminated. Furthermore, particle charge calculation based on particle deflection velocity neglected the particle drag force and rebound effect of the highly charged particles from the electrodes. We improved upon the existing particle charge measurement method by: 1) hologram post processing, 2) taking drag force into account in charge calculation, 3) considering rebound effect. The improved method was first fine-tuned through a calibration experiment. The complete method was then applied to two different experiments, namely conduction charging and enclosed fan-driven turbulence chamber, to measure particle charges. In all three experiments conducted, the particle charge was found to obey non-central t-location scale family of distribution. It was also noted that the charge distribution was insensitive to the change in voltage applied between the electrodes. The range of voltage

  13. Thermodynamic holography.

    PubMed

    Wei, Bo-Bo; Jiang, Zhan-Feng; Liu, Ren-Bao

    2015-01-01

    The holographic principle states that the information about a volume of a system is encoded on the boundary surface of the volume. Holography appears in many branches of physics, such as optics, electromagnetism, many-body physics, quantum gravity, and string theory. Here we show that holography is also an underlying principle in thermodynamics, a most important foundation of physics. The thermodynamics of a system is fully determined by its partition function. We prove that the partition function of a finite but arbitrarily large system is an analytic function on the complex plane of physical parameters, and therefore the partition function in a region on the complex plane is uniquely determined by its values along the boundary. The thermodynamic holography has applications in studying thermodynamics of nano-scale systems (such as molecule engines, nano-generators and macromolecules) and provides a new approach to many-body physics. PMID:26478214

  14. Thermodynamic holography

    PubMed Central

    Wei, Bo-Bo; Jiang, Zhan-Feng; Liu, Ren-Bao

    2015-01-01

    The holographic principle states that the information about a volume of a system is encoded on the boundary surface of the volume. Holography appears in many branches of physics, such as optics, electromagnetism, many-body physics, quantum gravity, and string theory. Here we show that holography is also an underlying principle in thermodynamics, a most important foundation of physics. The thermodynamics of a system is fully determined by its partition function. We prove that the partition function of a finite but arbitrarily large system is an analytic function on the complex plane of physical parameters, and therefore the partition function in a region on the complex plane is uniquely determined by its values along the boundary. The thermodynamic holography has applications in studying thermodynamics of nano-scale systems (such as molecule engines, nano-generators and macromolecules) and provides a new approach to many-body physics. PMID:26478214

  15. Superresolution imaging system by color-coded tilted-beam illumination in digital in-line holographic microscopy

    NASA Astrophysics Data System (ADS)

    Granero, L.; Micó, V.; Ferreira, C.; Zalevsky, Z.; García, J.

    2016-04-01

    Digital in-line holographic microscopy (DIHM) relates with the capability to achieve microscopic imaging working without lensless in the regime of holography. In essence, DIHM proposes a simple layout where a point source of coherent light illuminates the sample and the diffracted wavefront is recorded by a digital sensor. However, DIHM lacks high numerical aperture (NA) due to both geometrical distortion and the mandatory compromise between the illumination pinhole diameter, the illumination wavelength, and the need to obtain a reasonable light efficiency. One way to improve the resolution in DIHM, is by allowing superresolution imaging by angular multiplexing using tilted beam illumination. This illumination allows the on-axis diffraction of different spatial frequency content of the sample's spectrum, different in comparison to the case when on-axis illumination is used. And after recover this additional spectral content, a synthetic numerical aperture (SNA) expanding up the cutoff frequency of the system in comparison with the on-axis illumination case can be assembled in a digital post-processing stage. In this contribution, we present a method to achieve one-dimensional (1-D) superresolved imaging in DIHM by a SINGLE SHOT illumination, using color-coded tilted beams. The method has been named as L-SESRIM (Lensless Single-Exposure Super-Resolved Interferometric Microscopy). Although the technique was previously presented showing very preliminary results [34], in this contribution we expand the experimental characterization (USAF resolution test target) as well as derive the theoretical frame for SNA generation using different illumination wavelengths.

  16. Testing an in-line digital holography ‘inverse method’ for the Lagrangian tracking of evaporating droplets in homogeneous nearly isotropic turbulence

    NASA Astrophysics Data System (ADS)

    Chareyron, D.; Marié, J. L.; Fournier, C.; Gire, J.; Grosjean, N.; Denis, L.; Lance, M.; Méès, L.

    2012-04-01

    An in-line digital holography technique is tested, the objective being to measure Lagrangian three-dimensional (3D) trajectories and the size evolution of droplets evaporating in high-Reλ strong turbulence. The experiment is performed in homogeneous, nearly isotropic turbulence (50 × 50 × 50 mm3) created by the meeting of six synthetic jets. The holograms of droplets are recorded with a single high-speed camera at frame rates of 1-3 kHz. While hologram time series are generally processed using a classical approach based on the Fresnel transform, we follow an ‘inverse problem’ approach leading to improved size and 3D position accuracy and both in-field and out-of-field detection. The reconstruction method is validated with 60 μm diameter water droplets released from a piezoelectric injector ‘on-demand’ and which do not appreciably evaporate in the sample volume. Lagrangian statistics on 1000 reconstructed tracks are presented. Although improved, uncertainty on the depth positions remains higher, as expected in in-line digital holography. An additional filter is used to reduce the effect of this uncertainty when calculating the droplet velocities and accelerations along this direction. The diameters measured along the trajectories remain constant within ±1.6%, thus indicating that accuracy on size is high enough for evaporation studies. The method is then tested with R114 freon droplets at an early stage of evaporation. The striking feature is the presence on each hologram of a thermal wake image, aligned with the relative velocity fluctuations ‘seen’ by the droplets (visualization of the Lagrangian fluid motion about the droplet). Its orientation compares rather well with that calculated by using a dynamical equation for describing the droplet motion. A decrease of size due to evaporation is measured for the droplet that remains longest in the turbulence domain.

  17. High resolution x-ray lensless imaging by differential holographic encoding

    SciTech Connect

    Zhu, D.; Guizar-Sicairos, M.; Wu, B.; Scherz, A.; Acremann, Y.; Tylisczcak, T.; Fischer, P.; Friedenberger, N.; Ollefs, K.; Farle, M.; Fienup, J. R.; Stohr, J.

    2009-11-02

    X-ray free electron lasers (X-FEL{sub s}) will soon offer femtosecond pulses of laterally coherent x-rays with sufficient intensity to record single-shot coherent scattering patterns for nanoscale imaging. Pulse trains created by splitand-delay techniques even open the door for cinematography on unprecedented nanometer length and femtosecond time scales. A key to real space ultrafast motion pictures is fast and reliable inversion of the recorded reciprocal space scattering patterns. Here we for the first time demonstrate in the x-ray regime the power of a novel technique for lensless high resolution imaging, previously suggested by Guizar-Sicairos and Fienup termed holography with extended reference by autocorrelation linear differential operation, HERALD0. We have achieved superior resolution over conventional x-ray Fourier transform holography (FTH) without sacrifices in SNR or significant increase in algorithmic complexity. By combining images obtained from individual sharp features on an extended reference, we further show that the resolution can be even extended beyond the reference fabrication limits. Direct comparison to iterative phase retrieval image reconstruction and images recorded with stateof- the-art zone plate microscopes is presented. Our results demonstrate the power of HERALDO as a favorable candidate for robust inversion of single-shot coherent scattering patterns.

  18. Photoelectron Holography

    NASA Astrophysics Data System (ADS)

    Huismans, Ymkje

    2011-05-01

    New techniques using High Harmonic Generation (HHG) or attosecond pulses have proven to be successful in following the ultrafast motion of electrons and holes inside a molecule,. We introduce a complementary technique; photoelectron holography. This uses the phase and amplitude of the rescattered electrons to encode the structure and dynamics of the studied atom or molecule. Since photoelectron holography benefits from longer wavelengths, i.e. small photon energies, it is very suitable for systems with a small ionization potential. To demonstrate photoelectron holography, both measurements and calculations on atomic Xenon will be shown. Metastable Xenon was ionized with 7 μm light from the FELICE-free electron laser. The three dimensional momentum distribution of the photoelectrons was recorded by a Velocity Map Imaging (VMI)-spectrometer. In these momentum maps interference structures are observed that can be identified as an interference of direct and scattered electrons; a hologram of Xenon. Semi-classical calculations have demonstrated that in the hologram dynamical information of the electron and the atom is stored with a femtosecond to attosecond time resolution. Supervisor: Prof. Dr. M.J.J. Vrakking

  19. High-resolution digital holographic imaging by using a spatial light modulator

    NASA Astrophysics Data System (ADS)

    Li, Bin; Wang, Da-Yong; Wang, Yun-Xin; Rong, Lu

    2014-09-01

    Digital holography is the product of the optical holography, computer technology and photoelectric detection technology, and has the advantage of high-speed, real-time, full field of view, non-contact and quantitative phase contrast imaging. However, the numerical aperture of the hologram is limited due to the smaller sensitive area of the photoelectric sensor and the larger pixel size, and it is uneasy to meet the practical requirement on the imaging resolution. An approach is presented to achieve the high-resolution digital holographic imaging based on a spatial light modulator(SLM). An amplitude spatial light modulator is placed between the object and the CCD in the lensless Fourier transform digital holographic imaging system. The distribution of a diffraction grating is loaded into the SLM. In this way, more light including the high-frequency content, diffracted from the object, can be collected by the CCD. The standard resolution target is used as the object. The reconstructed image is obtained by the Fresnel diffraction propagation algorithm, which exhibits three diffraction orders. The results show that the resolution is improved from 62.5 μm to 31.3 μm.

  20. Parallel-mode scanning optical sectioning using digital Fresnel holography with three-wave interference phase-shifting.

    PubMed

    Kelner, Roy; Rosen, Joseph

    2016-02-01

    The Fresnel incoherent correlation holography (FINCH) method is applicable to various techniques of imaging, including fluorescence microscopy. Recently, a FINCH configuration capable of optical sectioning, using a scanning phase pinhole, has been suggested [Optica 1, 70 (2014)]. This capability is highly important in situations that demand the suppression of out-of-focus information from the hologram reconstruction of a specific plane of interest, such as the imaging of thick samples in biology. In this study, parallel-mode scanning using multiple phase pinholes is suggested as a means to shorten the acquisition time in an optical sectioning FINCH configuration. The parallel-mode scanning is enabled through a phase-shifting procedure that extracts the mixed term of two out of three interfering beams.

  1. Lensless imaging for wide field of view

    NASA Astrophysics Data System (ADS)

    Nagahara, Hajime; Yagi, Yasushi

    2015-02-01

    It is desirable to engineer a small camera with a wide field of view (FOV) because of current developments in the field of wearable cameras and computing products, such as action cameras and Google Glass. However, typical approaches for achieving wide FOV, such as attaching a fisheye lens and convex mirrors, require a trade-off between optics size and the FOV. We propose camera optics that achieve a wide FOV, and are at the same time small and lightweight. The proposed optics are a completely lensless and catoptric design. They contain four mirrors, two for wide viewing, and two for focusing the image on the camera sensor. The proposed optics are simple and can be simply miniaturized, since we use only mirrors for the proposed optics and the optics are not susceptible to chromatic aberration. We have implemented the prototype optics of our lensless concept. We have attached the optics to commercial charge-coupled device/complementary metal oxide semiconductor cameras and conducted experiments to evaluate the feasibility of our proposed optics.

  2. High-speed (20  kHz) digital in-line holography for transient particle tracking and sizing in multiphase flows.

    PubMed

    Guildenbecher, Daniel R; Cooper, Marcia A; Sojka, Paul E

    2016-04-10

    High-speed (20 kHz) digital in-line holography (DIH) is applied for 3D quantification of the size and velocity of fragments formed from the impact of a single water drop onto a thin film of water and burning aluminum particles from the combustion of a solid rocket propellant. To address the depth-of-focus problem in DIH, a regression-based multiframe tracking algorithm is employed, and out-of-plane experimental displacement accuracy is shown to be improved by an order-of-magnitude. Comparison of the results with previous DIH measurements using low-speed recording shows improved positional accuracy with the added advantage of detailed resolution of transient dynamics from single experimental realizations. The method is shown to be particularly advantageous for quantification of particle mass flow rates. For the investigated particle fields, the mass flows rates, which have been automatically measured from single experimental realizations, are found to be within 8% of the expected values.

  3. High-speed (20 kHz) digital in-line holography for transient particle tracking and sizing in multiphase flows

    DOE PAGESBeta

    Guildenbecher, Daniel R.; Cooper, Marcia A.; Sojka, Paul E.

    2016-04-05

    High-speed (20 kHz) digital in-line holography (DIH) is applied for 3D quantification of the size and velocity of fragments formed from the impact of a single water drop onto a thin film of water and burning aluminum particles from the combustion of a solid rocket propellant. To address the depth-of-focus problem in DIH, a regression-based multiframe tracking algorithm is employed, and out-of-plane experimental displacement accuracy is shown to be improved by an order-of-magnitude. Comparison of the results with previous DIH measurements using low-speed recording shows improved positional accuracy with the added advantage of detailed resolution of transient dynamics from singlemore » experimental realizations. Furthermore, the method is shown to be particularly advantageous for quantification of particle mass flow rates. For the investigated particle fields, the mass flows rates, which have been automatically measured from single experimental realizations, are found to be within 8% of the expected values.« less

  4. Incoherent holography

    NASA Astrophysics Data System (ADS)

    Abramson, Nils H.

    2000-10-01

    Dennis Gabor invented in-line holography in 1947, but at that time the coherent light from a laser did not yet exist and therefore the holograms he produced were of very low quality. When the laser was born in 1960 beautiful 3-D off- center holograms were for the first time produced by Emmett Leith and Juris Upatnieks. However, already as early as 1934 the inventor and artist Hans Weil patented a method to produce simple pictures that appeared floating in space, by scratching a transparent or metallic surface in certain directions. In 1995 William J. Beaty published a method for Hand-Drawn Holograms. Then it became possible for any artist to draw his own 3-D pictures of simple objects and using his ingenious techniques these hand drawn images will mimic many of the qualities of ordinary holograms.

  5. Holography: A survey

    NASA Technical Reports Server (NTRS)

    Dudley, D. D.

    1973-01-01

    The development of holography and the state of the art in recording and displaying information, microscopy, motion, pictures, and television applications are discussed. In addition to optical holography, information is presented on microwave, acoustic, ultrasonic, and seismic holography. Other subjects include data processing, data storage, pattern recognition, and computer-generated holography. Diagrams of holographic installations are provided. Photographs of typical holographic applications are used to support the theoretical aspects.

  6. Holography and tomography

    SciTech Connect

    Howells, M.

    1997-02-01

    This session includes a collection of outlines of pertinent information, diagrams, graphs, electron micrographs, and color photographs pertaining to historical aspects and recent advances in the development of X-ray Gabor Holography. Many of the photographs feature or pertain to instrumentation used in holography, tomography, and cryo-holography.

  7. Single-exposure super-resolved interferometric microscopy by RGB multiplexing in lensless configuration

    NASA Astrophysics Data System (ADS)

    Granero, Luis; Ferreira, Carlos; Zalevsky, Zeev; García, Javier; Micó, Vicente

    2016-07-01

    Single-Exposure Super-Resolved Interferometric Microscopy (SESRIM) reports on a way to achieve one-dimensional (1-D) superresolved imaging in digital holographic microscopy (DHM) by a single illumination shot and digital recording. SESRIM provides color-coded angular multiplexing of the accessible sample's range of spatial frequencies and it allows their recording in a single CCD (color or monochrome) snapshot by adding 3 RGB coherent reference beams at the output plane. In this manuscript, we extend the applicability of SESRIM to the field of digital in-line holographic microscopy (DIHM), that is, working without lenses. As consequence of the in-line configuration, an additional restriction concerning the object field of view (FOV) must be imposed to the technique. Experimental results are reported for both a synthetic object (USAF resolution test target) and a biological sample (swine sperm sample) validating this new kind of superresolution imaging method named as lensless SESRIM (L-SESRIM).

  8. A new wavelet-based reconstruction algorithm for twin image removal in digital in-line holography

    NASA Astrophysics Data System (ADS)

    Hattay, Jamel; Belaid, Samir; Aguili, Taoufik; Lebrun, Denis

    2016-07-01

    Two original methods are proposed here for digital in-line hologram processing. Firstly, we propose an entropy-based method to retrieve the focus plane which is very useful for digital hologram reconstruction. Secondly, we introduce a new approach to remove the so-called twin images reconstructed by holograms. This is achieved owing to the Blind Source Separation (BSS) technique. The proposed method is made up of two steps: an Adaptive Quincunx Lifting Scheme (AQLS) and a statistical unmixing algorithm. The AQLS tool is based on wavelet packet transform, whose role is to maximize the sparseness of the input holograms. The unmixing algorithm uses the Independent Component Analysis (ICA) tool. Experimental results confirm the ability of convolutive blind source separation to discard the unwanted twin image from in-line digital holograms.

  9. Noise suppression in curved glass shells using macro-fiber-composite actuators studied by the means of digital holography and acoustic measurements

    NASA Astrophysics Data System (ADS)

    Mokrý, P.; Psota, P.; Steiger, K.; Václavík, J.; Doleček, R.; Lédl, V.; Šulc, M.

    2015-02-01

    The paper presents methods and experimental results of the semi-active control of noise transmission in a curved glass shell with attached piezoelectric macro fiber composite (MFC) actuators. The semi-active noise control is achieved via active elasticity control of piezoelectric actuators by connecting them to an active electric shunt circuit that has a negative effective capacitance. Using this approach, it is possible to suppress the vibration of the glass shell in the normal direction with respect to its surface and to increase the acoustic transmission loss of the piezoelectric MFC-glass composite structure. The effect of the MFC actuators connected to the negative capacitance shunt circuit on the surface distribution of the normal vibration amplitude is studied using frequency-shifted digital holography (FSDH). The principle of the used FSDH method is described in the paper. The frequency dependence of the acoustic transmission loss through the piezoelectric MFC-glass composite structure is estimated using measurements of the specific acoustic impedance of the curved glass shell. The specific acoustic impedance is measured using two microphones and a laser Doppler vibrometer (LDV). The results from the LDV measurements are compared with the FSDH data. The results of the experiments show that using this approach, the acoustic transmission loss in a glass shell can be increased by 36 dB in the frequency range around 247 Hz and by 25 dB in the frequency range around 258 Hz. The experiments indicate that FSDH measurements provide an efficient tool that can be used for fast and accurate measurements of the acoustic transmission loss in large planar structures.

  10. Spatial crystal imaging by means of atomic electron holography

    NASA Astrophysics Data System (ADS)

    Luehr, Tobias; Winkelmann, Aimo; Nolze, Gert; Westphal, Carsten

    2015-03-01

    The determination of atom structures is the key for the understanding of basic functional properties of matter or for designing new high-tech materials. For structure determination, holography is a very attractive option, since this method enables lensless three-dimensional imaging. In principle, x-ray photoelectron diffraction (XPD) experiments correspond to a microscopic holography setup. The electron diffraction pattern is highly sensitive to the local structure of the emitter environment, since the emitting atom is located in the near-field of the scattering atoms. Hence, holographic reconstructions of XPD-patterns should yield a spatial image of the sample's atom arrangement. However, anisotropic electron scattering and multiple scattering effects generally cause strong artifacts in the reconstruction. In this contribution we show how to circumvent this problem with electrons at kinetic energies of Ekin >= 10 keV. The resulting spatial images contain hundreds of clearly separated atoms at their correct locations. Furthermore, XPD-patterns allow an element-specific assignment of the reconstructed atoms within the image. This is the first demonstration of true atom imaging following Dennis Gabor's idea of electron holography.

  11. Impact of noise in holography with extended references in the low signal regime.

    PubMed

    Boutu, W; Gauthier, D; Ge, X; Cassin, R; Ducousso, M; Gonzalez, A I; Iwan, B; Samaan, J; Wang, F; Kovačev, M; Merdji, H

    2016-03-21

    Signal-to-noise ratio is a key factor in lensless imaging, particularly for low diffraction signal experiments in the single shot regime. We present our recent study of the noise impact on holography with extended references. Experimental data have been measured in single shot acquisition using an intense coherent soft X-ray high harmonic source. The impact of hardware and software noise under various detection conditions is discussed. A final comparison between single shot and multi-shot regimes is given. PMID:27136823

  12. On the Diffraction Limit for Lensless Imaging

    PubMed Central

    Mielenz, Klaus D.

    1999-01-01

    The diffraction limit for lensless imaging, defined as the sharpest possible point image obtainable with a pinhole aperture, is analyzed and compared to the corresponding limit for imaging with lenses by means of theoretical considerations and numerical computations using the Fresnel-Lommel diffraction theory for circular apertures. The numerical result (u = π) obtained for the best configuration parameter u which defines the optical setup is consistent with the quarter-wave criterion, and is the same as the value reported in a classical paper by Petzval but smaller than the value (u = 1.8π) found by Lord Rayleigh. The smallest discernible detail (pixel) in a composite image is defined by an expression found by Rayleigh on applying the half-wave criterion and is shown to be consistent with the Sparrow criterion of resolution. The numerical values of other measures of image size are reported and compared to equivalent parameters of the Fraunhofer-Airy profile that governs imaging with lenses.

  13. Lensless magneto-optic speed sensor

    DOEpatents

    Veeser, L.R.; Forman, P.R.; Rodriguez, P.J.

    1998-02-17

    Lensless magneto-optic speed sensor is disclosed. The construction of a viable Faraday sensor has been achieved. Multimode fiber bundles are used to collect the light. If coupled directly into a 100 or 200 {micro}m core fiber, light from a light emitting diode (LED) is sufficient to operate the sensor. In addition, LEDs ensure that no birefringence effects in the input fiber are possible, as the output from such light sources have random polarization. No lens is required since the large diameter optical fibers and thin crystals of materials having high Verdet constants (such as iron garnets) employed permit the collection of a substantial quantity of light. No coupler is required. The maximum amount of light which could reach a detector using a coupler is 25%, while the measured throughput of the fiber-optic bundle without a coupler is about 42%. All of the elements employed in the present sensor are planar, and no particular orientation of these elements is required. The present sensor operates over a wide range of distances from magnetic field sources, and observed signals are large. When a tone wheel is utilized, the signals are independent of wheel speed, and the modulation is observed to be about 75%. No sensitivity to bends in the input or output optical fiber leads was observed. Reliable operation was achieved down to zero frequency, or no wheel rotation. 5 figs.

  14. Lensless Magneto-optic speed sensor

    DOEpatents

    Veeser, Lynn R.; Forman, Peter R.; Rodriguez, Patrick J.

    1998-01-01

    Lensless magneto-optic speed sensor. The construction of a viable Faraday sensor has been achieved. Multimode fiber bundles are used to collect the light. If coupled directly into a 100 or 200 .mu.m core fiber, light from a light emitting diode (LED) is sufficient to operate the sensor. In addition, LEDs ensure that no birefringence effects in the input fiber are possible, as the output from such light sources have random polarization. No lens is required since the large diameter optical fibers and thin crystals of materials having high Verdet constants (such as iron garnets) employed permit the collection of a substantial quantity of light. No coupler is required. The maximum amount of light which could reach a detector using a coupler is 25%, while the measured throughput of the fiber-optic bundle without a coupler is about 42%. All of the elements employed in the present sensor are planar, and no particular orientation of these elements is required. The present sensor operates over a wide range of distances from magnetic field sources, and observed signals are large. When a tone wheel is utilized, the signals are independent of wheel speed, and the modulation is observed to be about 75%. No sensitivity to bends in the input or output optical fiber leads was observed. Reliable operation was achieved down to zero frequency, or no wheel rotation.

  15. Lensless imaging system to quantify cell proliferation

    NASA Astrophysics Data System (ADS)

    Vinjimore Kesavan, S.; Allier, C. P.; Navarro, F.; Mittler, F.; Chalmond, B.; Dinten, J.-M.

    2013-02-01

    Owing to its simplicity, lensless imaging system is adept at continuous monitoring of adherent cells inside the incubator. The setup consists of a CMOS sensor with pixel pitch of 2.2 μm and field of view of 24 mm2, LED with a dominating wavelength of 525 nm, along with a pinhole of 150 μm as the source of illumination. The in-line hologram obtained from cells depends on the degree of cell-substrate adhesion. Drastic difference is observed between the holographic patterns of floating and adherent cells. In addition, the well-established fact of reduction of cell-substrate contact during cell division is observed with our system based on corresponding spontaneous transition in the holographic pattern. Here, we demonstrate that by recognizing this specific holographic pattern, number of cells undergoing mitosis in a cell culture with a population of approximately 5000 cells, can be estimated in real-time. The method is assessed on comparison with Edu-based proliferation assay. The approach is straightforward and it eliminates the use of markers to estimate the proliferation rate of a given cell culture. Unlike most proliferation assays, the cells are not harvested enabling continuous monitoring of cell culture.

  16. Measurement of three-dimensional stress field of RP model having a notch by using digital holography

    NASA Astrophysics Data System (ADS)

    Tanak, Y.; Murata, S.

    2010-06-01

    We measure three-dimensional stress field of RP model having a V-notch. RP model (Elastic modulues E = 3317 MPa, 9.3 × 7.7 × 50 mm3 having a V-notch) is made of acrylic transparent resin and tracer particle (averaged diameter: 60μm) are dispersed. The model is subjected to the static load (100 N) at the middle. Firstly, three-dimensional deflection is measured by using digital holographic PTV (Particle Tracking Velocimetry). Finally, three-dimensional stress field of RP model is visualized as a differential value of the deflection field.

  17. Holography-guided ptychography with soft X-rays.

    PubMed

    Hessing, Piet; Pfau, Bastian; Guehrs, Erik; Schneider, Michael; Shemilt, Laura; Geilhufe, Jan; Eisebitt, Stefan

    2016-01-25

    Ptychography is a lensless imaging technique that aims to reconstruct an object from a set of coherent diffraction patterns originating from different and partially overlapping sample illumination areas. For a successful convergence of the iterative algorithms used, the sample scan positions have to be known with very high accuracy. Here, we present a method that allows to directly encode this information in the diffraction patterns without the need of accurate position encoders. Our approach relies on combining ptychography with another coherent imaging method, namely Fourier-transform holography. We have imaged two different objects using coherent soft-X-ray illumination and investigate the influence of experimental and numerical position refinement on the reconstruction result. We demonstrate that holographically encoded positions significantly reduce the experimental and numerical requirements. Our ptychographic reconstructions cover a large field of view with diffraction-limited resolution and high sensitivity in the reconstructed phase shift and absorption of the objects.

  18. Color holography: recent improvements and applications

    NASA Astrophysics Data System (ADS)

    Bjelkhagen, Hans I.

    2013-03-01

    A review of recent improvements and applications in color holography is provided. Color holography recording techniques in silver-halide emulsions and photopolymer materials are discussed. Both analogue Denisyuk color holograms and digitally-printed color holograms are described. The light sources used to illuminate the recorded holograms are very important to obtain ultra-realistic 3D images. In particular the new light sources based on RGB LEDs are significant improvements in displaying color holograms with improved image quality over today's commonly used halogen lights. Color holograms of museum artifacts have been recorded with new mobile holographic equipment.

  19. Double images hiding by using joint transform correlator architecture adopting two-step phase-shifting digital holography

    NASA Astrophysics Data System (ADS)

    Shi, Xiaoyan; Zhao, Daomu; Huang, Yinbo

    2013-06-01

    Based on the joint Fresnel transform correlator, a new system for double images hiding is presented. By this security system, the dual secret images are encrypted and recorded as intensity patterns employing phase-shifting interference technology. To improve the system security, a dual images hiding method is used. By digital means, the deduced encryption complex distribution is divided into two subparts. For each image, only one subpart is reserved and modulated by a phase factor. Then these modified results are combined together and embedded into the host image. With all correct keys, by inverse Fresnel transform, the secret images can be extracted. By the phase modulation, the cross talk caused by images superposition can be reduced for their spatial parallel separation. Theoretical analyses have shown the system's feasibility. Computer simulations are performed to show the encryption capacity of the proposed system. Numerical results are presented to verify the validity and the efficiency of the proposed method.

  20. Underwater holography: past and future

    NASA Astrophysics Data System (ADS)

    Watson, John

    2006-05-01

    100 m and over 300 holograms recorded. However, the HoloMar camera is physically large and heavy and difficult to deploy. It is also based on the use of photographic emulsions to record the holograms. To overcome some of these difficulties we are now developing a new holographic camera (eHoloCam) based on digital holography. In digital or "eHolography", a hologram is directly electronically recorded onto a CCD or CMOS sensor and then numerically reconstructed by simulation of the optical hologram reconstruction. The immediate advantages of this new camera are compactness, ease-of-use and speed of response, but at the expense of restricted off-axis recording angles and reduced recording volume. In this paper we describe both approaches, the use of holography for analysis of marine organisms and the results obtained in the field. We also describe recent work, using both photo and digital holography, to study the behaviour of sediments in river estuaries and outline future applications of underwater holography.

  1. Labeling technique for nonplanar surfaces based on the combination of a diffractive axilens with digital holography methods

    NASA Astrophysics Data System (ADS)

    Marin, Pablo; Lizana, Angel; Peinado, Alba; Mora-González, Miguel; Campos, Juan

    2015-05-01

    A technique based on a spatial light modulator is proposed for the labeling of nonplanar surfaces. This technique arises from the combination of a general method for generation of digital holograms with a diffractive axilens. Unlike existing methods in which hologram contrast critically decreases out of the focal plane, our proposed approach allows the system to increase the focus depth, resulting in enhanced hologram images out of the focal plane. Two existing methods for hologram generation are theoretically and experimentally compared in terms of efficiency and contrast: the iterative Fourier transform algorithm-based method and the random multiplexing of different lenses method. The most suitable one is selected for the implementation of the new axilens-based technique. Experimental results obtained with the axilens method provide a significant enhancement of the image quality of holograms obtained out of the focal plane when compared with an existing technique. The approach proposed in this manuscript may be of interest in industrial applications, where alphanumeric data must be registered on nonflat surfaces, as in the cases of beverage cans or bottles.

  2. Widefield lensless imaging through a fiber bundle via speckle correlations.

    PubMed

    Porat, Amir; Andresen, Esben Ravn; Rigneault, Hervé; Oron, Dan; Gigan, Sylvain; Katz, Ori

    2016-07-25

    Flexible fiber-optic endoscopes provide a solution for imaging at depths beyond the reach of conventional microscopes. Current endoscopes require focusing and/or scanning mechanisms at the distal end, which limit miniaturization, frame-rate, and field of view. Alternative wavefront-shaping based lensless solutions are extremely sensitive to fiber-bending. We present a lensless, bend-insensitive, single-shot imaging approach based on speckle-correlations in fiber bundles that does not require wavefront shaping. Our approach computationally retrieves the target image by analyzing a single camera frame, exploiting phase information that is inherently preserved in propagation through convnetional fiber bundles. Unlike conventional fiber-based imaging, planar objects can be imaged at variable working distances, the resulting image is unpixelated and diffraction-limited, and miniaturization is limited only by the fiber diameter. PMID:27464136

  3. Lensless ghost imaging through the strongly scattering medium

    NASA Astrophysics Data System (ADS)

    Zhe, Yang; Lianjie, Zhao; Xueliang, Zhao; Wei, Qin; Junlin, Li

    2016-02-01

    Lensless ghost imaging has attracted much interest in recent years due to its profound physics and potential applications. In this paper we report studies of the robust properties of the lensless ghost imaging system with a pseudo-thermal light source in a strongly scattering medium. The effects of the positions of the strong medium on the ghost imaging are investigated. In the lensless ghost imaging system, a pseudo-thermal light is split into two correlated beams by a beam splitter. One beam goes to a charge-coupled detector camera, labeled as CCD2. The other beam goes to an object and then is collected in another charge-coupled detector camera, labeled as CCD1, which serves as a bucket detector. When the strong medium, a pane of ground glass disk, is placed between the object and CCD1, the bucket detector, the quality of ghost imaging is barely affected and a good image could still be obtained. The quality of the ghost imaging can also be maintained, even when the ground glass is rotating, which is the strongest scattering medium so far. However, when the strongly scattering medium is present in the optical path from the light source to CCD2 or the object, the lensless ghost imaging system hardly retrieves the image of the object. A theoretical analysis in terms of the second-order correlation function is also provided. Project supported by the National Natural Science Foundation of China (Grant Nos. 11175094 and 91221205), the National Basic Research Program of China (Grant No. 2015CB921002), and partially supported by the Basic Research Fund of Beijing Institute of Technology (Grant No. 20141842005).

  4. Lensless ghost imaging based on mathematical simulation and experimental simulation

    NASA Astrophysics Data System (ADS)

    Liu, Yanyan; Wang, Biyi; Zhao, Yingchao; Dong, Junzhang

    2014-02-01

    The differences of conventional imaging and correlated imaging are discussed in this paper. The mathematical model of lensless ghost imaging system is set up and the image of double slits is computed by mathematical simulation. The results are also testified by the experimental verification. Both the theory simulation and experimental verifications results shows that the mathematical model based on statistical optical principle are keeping consistent with real experimental results.

  5. Haptic Holography/Touching the Ethereal

    NASA Astrophysics Data System (ADS)

    Page, Michael

    2013-02-01

    Haptic Holography, was perhaps, first proposed by workers at MIT in the 90s. The Media Lab, headed up by Dr. Stephen Benton, with published papers by Wendy Plesiak and Ravi Pappuh. -1 Recent developments in both the technology of digital holography and haptics have made it practical to conduct further investigations. Haptic holography is auto-stereoscopic and provides co-axial viewing for the user. Haptic holography may find application in medical & surgical training and as a new form of synthetic reality for artists and designers. At OCAD's PHASE Lab (Prototypes for Holographic Art and Science Explorations) workers are exploring hybrid forms of augmented reality, that combine haptics, interactivity and auto-stereoscopic imagery. Conventional Haptic environments, while presenting a 3D physics environment, typically provide a 2D visual work/play space. Orienteering in such an environment creates an uncertain spatial relationship for the user. Our group creates 3d models from which we create holographic constructs. The same model is used to create the physics environment. The two models are super-imposed. The result: Holograms you can touch.

  6. An Introduction to Educational Holography.

    ERIC Educational Resources Information Center

    Lloyd, R. Scott

    Holograms are capable of taking the two-dimensional ways of envisioning information to another dimension of presentation, representation, and conceptualization. Educational holography is joining display holography, holographic testing of materials, and holographic optical elements as a fourth major field in holography. Holograms are explored as…

  7. Experimental characterization of the hygroscopic properties of wood during convective drying using digital holographic interferometry.

    PubMed

    Kumar, Manoj; Shakher, Chandra

    2016-02-10

    In this paper, an application of digital holography for the measurement of surface deformations and the strain field to understand the shrinkage behavior of wood during convective drying is presented. Moisture absorption and desorption induce the dimensional changes and deformations in wood that leads to failure of certain components made of wood. The knowledge of the dimensional changes in wood, deformations, strain distribution and their causes are important for the best utilization of wood. For the study, lensless Fourier transform digital holographic interferometry is used to measure moisture- induced deformation, strain distribution, and the coefficient of hygroscopic shrinkage in different samples of wood. The technique is highly sensitive and enables the observation of deformation and strain distribution during the variations of moisture content in the wood. The wet wood sample was exposed to convective drying, which leads to changes in the moisture content and the associated deformations. The deformation/strain in each step of drying process is used to evaluate the coefficient of hygroscopic shrinkage in different wood samples. The experiments were repeated for differently treated woods. The experimental results show that the strain and coefficient of hygroscopic shrinkage can be minimized if the wood is dried in the presence of the proper moisture content. PMID:26906359

  8. Holography and Optical Storage

    NASA Astrophysics Data System (ADS)

    Imlau, Mirco; Fally, Martin; Burr, Geoffrey W.; Sincerbox, Glenn T.

    The term holography is composed of the Greek words holos (= whole) and graphein (= to record, to write), and thus summarizes the key aspects of its underlying principle: recording the complete wavefront of an object, i.e., its intensity as well as its phase. Interference and diffraction phenomena are employed to record and retrieve the full information, a technique pioneered by Dennis Gabor in 1948. He was honored with the Nobel prize in Physics in 1971, reflecting the general impact of holography on modern physics.

  9. Virtual integral holography

    NASA Astrophysics Data System (ADS)

    Venolia, Dan S.; Williams, Lance

    1990-08-01

    components of a stereo display system with user point-of-view tracking for interactive 3D, and a digital realization of integral composite display which we term virtual integral holography. The primary drawbacks of holographic display - film processing turnaround time, and the difficulties of displaying scenes in full color -are obviated, and motion parallax cues provide easy 3D interpretation even for users who cannot see in stereo.

  10. Holography without Fuss.

    ERIC Educational Resources Information Center

    Davies, Steve

    1989-01-01

    Outlines what a hologram is, the main types of holography, and how a simple system producing a white light reflection hologram can be set up in a school physics laboratory. Discusses the basic optics of the hologram and procedures and materials for making holograms in school. (YP)

  11. Fast wideband acoustical holography.

    PubMed

    Hald, Jørgen

    2016-04-01

    Patch near-field acoustical holography methods like statistically optimized near-field acoustical holography and equivalent source method are limited to relatively low frequencies, where the average array-element spacing is less than half of the acoustic wavelength, while beamforming provides useful resolution only at medium-to-high frequencies. With adequate array design, both methods can be used with the same array. But for holography to provide good low-frequency resolution, a small measurement distance is needed, whereas beamforming requires a larger distance to limit sidelobe issues. The wideband holography method of the present paper was developed to overcome that practical conflict. Only a single measurement is needed at a relatively short distance and a single result is obtained covering the full frequency range. The method uses the principles of compressed sensing: A sparse sound field representation is assumed with a chosen set of basis functions, a measurement is taken with an irregular array, and the inverse problem is solved with a method that enforces sparsity in the coefficient vector. Instead of using regularization based on the 1-norm of the coefficient vector, an iterative solution procedure is used that promotes sparsity. The iterative method is shown to provide very similar results in most cases and to be computationally much more efficient. PMID:27106299

  12. Lensless on-chip imaging of cells provides a new tool for high-throughput cell-biology and medical diagnostics.

    PubMed

    Mudanyali, Onur; Erlinger, Anthony; Seo, Sungkyu; Su, Ting-Wei; Tseng, Derek; Ozcan, Aydogan

    2009-12-14

    Conventional optical microscopes image cells by use of objective lenses that work together with other lenses and optical components. While quite effective, this classical approach has certain limitations for miniaturization of the imaging platform to make it compatible with the advanced state of the art in microfluidics. In this report, we introduce experimental details of a lensless on-chip imaging concept termed LUCAS (Lensless Ultra-wide field-of-view Cell monitoring Array platform based on Shadow imaging) that does not require any microscope objectives or other bulky optical components to image a heterogeneous cell solution over an ultra-wide field of view that can span as large as approximately 18 cm(2). Moreover, unlike conventional microscopes, LUCAS can image a heterogeneous cell solution of interest over a depth-of-field of approximately 5 mm without the need for refocusing which corresponds to up to approximately 9 mL sample volume. This imaging platform records the shadows (i.e., lensless digital holograms) of each cell of interest within its field of view, and automated digital processing of these cell shadows can determine the type, the count and the relative positions of cells within the solution. Because it does not require any bulky optical components or mechanical scanning stages it offers a significantly miniaturized platform that at the same time reduces the cost, which is quite important for especially point of care diagnostic tools. Furthermore, the imaging throughput of this platform is orders of magnitude better than conventional optical microscopes, which could be exceedingly valuable for high-throughput cell-biology experiments.

  13. Digital holographic measurements of shape and three-dimensional sound-induced displacements of tympanic membrane

    NASA Astrophysics Data System (ADS)

    Khaleghi, Morteza; Lu, Weina; Dobrev, Ivo; Cheng, Jeffrey Tao; Furlong, Cosme; Rosowski, John J.

    2013-10-01

    Acoustically induced vibrations of the tympanic membrane (TM) play a primary role in the hearing process, in that these motions are the initial mechanical response of the ear to airborne sound. Characterization of the shape and three-dimensional (3-D) displacement patterns of the TM is a crucial step to a better understanding of the complicated mechanics of sound reception by the ear. Sound-induced 3-D displacements of the TM are estimated from shape and one-dimensional displacements measured in cadaveric chinchillas using a lensless dual-wavelength digital holography system (DWDHS). The DWDHS consists of laser delivery, optical head, and computing platform subsystems. Shape measurements are performed in double-exposure mode with the use of two wavelengths of a tunable laser, while nanometer-scale displacements are measured along a single sensitivity direction with a constant wavelength. Taking into consideration the geometrical and dimensional constrains imposed by the anatomy of the TM, we combine principles of thin-shell theory together with displacement measurements along a single sensitivity vector and TM surface shape to extract the three principal components of displacement in the full-field-of-view. We test, validate, and identify limitations of this approach via the application of finite element method to artificial geometries.

  14. Digital holographic measurements of shape and 3D sound-induced displacements of Tympanic Membrane

    PubMed Central

    Lu, Weina; Dobrev, Ivo; Cheng, Jeffrey Tao; Furlong, Cosme; Rosowski, John J

    2014-01-01

    Acoustically-induced vibrations of the Tympanic Membrane (TM) play a primary role in the hearing process, in that these motions are the initial mechanical response of the ear to airborne sound. Characterization of the shape and 3D displacement patterns of the TM is a crucial step to a better understanding of the complicated mechanics of sound reception by the ear. In this paper, shape and sound-induced 3D displacements of the TM in cadaveric chinchillas are measured by a lensless Dual-Wavelength Digital Holography system (DWDHS). The DWDHS consists of Laser Delivery (LD), Optical Head (OH), and Computing Platform (CP) subsystems. Shape measurements are performed in double-exposure mode and with the use of two wavelengths of a tunable laser while nanometer-scale displacements are measured along a single sensitivity direction and with a constant wavelength. In order to extract the three principal components of displacement in full-field-of-view, and taking into consideration the anatomical dimensions of the TM, we combine principles of thin-shell theory together with both, displacement measurements along the single sensitivity vector and TM surface shape. To computationally test this approach, Finite Element Methods (FEM) are applied to the study of artificial geometries. PMID:24790255

  15. Lensless hyperspectral spectromicroscopy with a tabletop extreme-ultraviolet source

    NASA Astrophysics Data System (ADS)

    Gardner, Dennis F.; Zhang, Bosheng; Seaberg, Matthew H.; Shanblatt, Elisabeth R.; Porter, Christina L.; Karl, Robert; Mancuso, Christopher; Kapteyn, Henry C.; Murnane, Margaret M.; Adams, Daniel E.

    2016-03-01

    We demonstrate hyperspectral coherent imaging in the EUV spectral region for the first time, without the need for hardware-based wavelength separation. This new scheme of spectromicroscopy is the most efficient use of EUV photons for imaging because there is no energy loss from mirrors or monochromatizing optics. An EUV spectral comb from a tabletop high-harmonic source, centered at a wavelength of 30nm, illuminates the sample and the scattered light is collected on a pixel-array detector. Using a lensless imaging technique known as ptychographical information multiplexing, we simultaneously retrieve images of the spectral response of the sample at each individual harmonic. We show that the retrieved spectral amplitude and phase agrees with theoretical predictions. This work demonstrates the power of coherent EUV beams for rapid material identification with nanometer-scale resolution.

  16. Medical applications of holography

    NASA Astrophysics Data System (ADS)

    von Bally, Gert

    1991-11-01

    From the various capabilities of holography for image processing and measuring purposes, holographic interferometric techniques have found more extended application in biological and medical research. Due to their special properties the different methods of holographic interferometry are applied to characteristic fields of biomedical investigations where--similar to nondestructive testing--vibration and deformation analysis is of interest. Features of holographic interferometry, such as the possibility of noncontactive, three-dimensional investigations with a large field-of-depth, are used with advantage. The main applications can be found in basic research e.g., in audiology, dentistry, opthalmology, and experimental orthopedics. Because of the great number of investigations and the variety of medical domains in which these investigations were performed this survey is confined to some characteristic examples. As in all fields of optics and laser metrology, a review on biomedical applications of holography would be incomplete if military developments and utilization were not mentioned. As demonstrated by selected examples, the increasing interlacing of science with the military does not stop at domains that traditionally are regarded as exclusively oriented to human welfare--like biomedical research. The term ''Star Wars Medicine'', which becomes an increasingly popular expression for laser applications (including holography) in medicine, characterizes the consequences of this development.

  17. New trend in electron holography

    NASA Astrophysics Data System (ADS)

    Tanigaki, Toshiaki; Harada, Ken; Murakami, Yasukazu; Niitsu, Kodai; Akashi, Tetsuya; Takahashi, Yoshio; Sugawara, Akira; Shindo, Daisuke

    2016-06-01

    Electron holography using a coherent electron wave is a promising technique for high-resolution visualization of electromagnetic fields in and around objects. The capability of electron holography has been enhanced by the development of new technologies and has thus become an even more powerful tool for exploring scientific frontiers. This review introduces these technologies including split-illumination electron holography and vector-field electron tomography. Split-illumination electron holography, which uses separated coherent waves, overcomes the limits imposed by the lateral coherence requirement for electron waves in electron holography. Areas that are difficult to observe using conventional electron holography are now observable. Exemplified applications include observing a singular magnetic domain wall in electrical steel sheets, local magnetizations at anti-phase boundaries, and electrostatic potentials in metal-oxide-semiconductor field-effect transistors. Vector-field electron tomography can be used to visualize magnetic vectors in three dimensions. Two components of the vectors are reconstructed using dual-axis tomography, and the remaining one is calculated using div B   =  0. A high-voltage electron microscope can be used to achieve precise magnetic reconstruction. For example, magnetic vortices have been visualized using a 1 MV holography electron microscope.

  18. Quantitative measurement of size and three-dimensional position of fast-moving bubbles in air-water mixture flows using digital holography.

    PubMed

    Tian, Lei; Loomis, Nick; Domínguez-Caballero, José A; Barbastathis, George

    2010-03-20

    We present a digital in-line holographic imaging system for measuring the size and three-dimensional position of fast-moving bubbles in air-water mixture flows. The captured holograms are numerically processed by performing a two-dimensional projection followed by local depth estimation to quickly and efficiently obtain the size and position information of multiple bubbles simultaneously. Statistical analysis on measured bubble size distributions shows that they follow lognormal or gamma distributions.

  19. Adaptive Optical Scanning Holography

    PubMed Central

    Tsang, P. W. M.; Poon, Ting-Chung; Liu, J.-P.

    2016-01-01

    Optical Scanning Holography (OSH) is a powerful technique that employs a single-pixel sensor and a row-by-row scanning mechanism to capture the hologram of a wide-view, three-dimensional object. However, the time required to acquire a hologram with OSH is rather lengthy. In this paper, we propose an enhanced framework, which is referred to as Adaptive OSH (AOSH), to shorten the holographic recording process. We have demonstrated that the AOSH method is capable of decreasing the acquisition time by up to an order of magnitude, while preserving the content of the hologram favorably. PMID:26916866

  20. Split-illumination electron holography

    SciTech Connect

    Tanigaki, Toshiaki; Aizawa, Shinji; Suzuki, Takahiro; Park, Hyun Soon; Inada, Yoshikatsu; Matsuda, Tsuyoshi; Taniyama, Akira; Shindo, Daisuke; Tonomura, Akira

    2012-07-23

    We developed a split-illumination electron holography that uses an electron biprism in the illuminating system and two biprisms (applicable to one biprism) in the imaging system, enabling holographic interference micrographs of regions far from the sample edge to be obtained. Using a condenser biprism, we split an electron wave into two coherent electron waves: one wave is to illuminate an observation area far from the sample edge in the sample plane and the other wave to pass through a vacuum space outside the sample. The split-illumination holography has the potential to greatly expand the breadth of applications of electron holography.

  1. Atomic resolution holography.

    PubMed

    Hayashi, Kouichi

    2014-11-01

    Atomic resolution holography, such as X-ray fluorescence holography (XFH)[1] and photoelectron holography (PH), has the attention of researcher as an informative local structure analysis, because it provides three dimensional atomic images around specific elements within a range of a few nanometers. It can determine atomic arrangements around a specific element without any prior knowledge of structures. It is considered that the atomic resolution holographic is a third method of structural analysis at the atomic level after X-ray diffraction (XRD) and X-ray absorption fine structure (XAFS). As known by many researchers, XRD and XAFS are established methods that are widespread use in various fields. XRD and XAFS provide information on long-range translational periodicities and very local environments, respectively, whereas the atomic resolution holography gives 3D information on the local order and can visualize surrounding atoms with a large range of coordination shells. We call this feature "3D medium-range local structure observation".In addition to this feature, the atomic resolution holography is very sensitive to the displacement of atoms from their ideal positions, and one can obtain quantitative information about local lattice distortions by analyzing reconstructed atomic images[2] When dopants with different atomic radii from the matrix elements are present, the lattices around the dopants are distorted. However, using the conventional methods of structural analysis, one cannot determine the extent to which the local lattice distortions are preserved from the dopants. XFH is a good tool for solving this problem.Figure 1 shows a recent achievement on a relaxor ferroelectric of Pb(Mg1/3Nb2/3)O3 (PMN) using XFH. The structural studies of relaxor ferroelectrics have been carried out by X-ray or neutron diffractions, which suggested rhombohedral distortions of their lattices. However, their true pictures have not been obtained, yet. The Nb Kα holograms showed

  2. Extended field-of-view in a lensless endoscope using an aperiodic multicore fiber

    NASA Astrophysics Data System (ADS)

    Sivankutty, Siddharth; Tsvirkun, Viktor; Bouwmans, Géraud; Kogan, Dani; Oron, Dan; Andresen, Esben Ravn; Rigneault, Hervé

    2016-08-01

    We investigate lensless endoscopy using coherent beam combining and aperiodic multicore fibers (MCF). We show that diffracted orders, inherent to MCF with periodically arranged cores, dramatically reduce the field of view (FoV) and that randomness in MCF core positions can increase the FoV up to the diffraction limit set by a single fiber core, while maintaining MCF experimental feasibility. We demonstrate experimentally pixelation-free lensless endoscopy imaging over a 120 micron FoV with an aperiodic MCF designed with widely spaced cores. We show that this system is suitable to perform beam scanning imaging by simply applying a tilt to the proximal wavefront.

  3. Measurements of polystyrene bead trajectories and spatial distributions in a turbulent water flow, square duct using high-speed digital holography

    NASA Astrophysics Data System (ADS)

    van Hout, Rene; Rabencov, Boris; Arca, Javier

    2014-11-01

    Near neutrally buoyant, polystyrene beads (583 micrometers) were tracked in a square (50 × 50 mm2), closed-loop, turbulent water duct at a bulk flow Reynolds number of 10,602 (friction velocity 0.0208 m/s) using single view, inline digital holographic cinematography (at 1 kHz). The volume of interest (50 × 17.4 × 17.4 mm3) was positioned at the bottom part of the channel. The mean bead diameter normalized by inner wall coordinates was d+ = 14.2, with Stokes numbers of 8.5. In-house developed algorithms, fine-tuned to tracking single and overlapping beads were developed. Bead in-focus positions were determined by maximum intensity gradient method. Results showed that in agreement with literature publications, ascending beads lagged the mean streamwise water velocity while descending ones had similar velocities. Average streamwise bead velocities and number densities collapsed onto wall-normal-streamwise and spanwise-streamwise planes, indicated preferential segregation of ascending and descending beads up to a height of 100 wall units. Spanwise ``lane'' separation distances ranged between 150-200 wall units, larger but of the same order as the spanwise extent of coherent near-wall turbulence structures. Duct corners were nearly devoid of beads likely caused by secondary flows. Israel Science Foundation Grant 915/10 and COST Actions MP0806 and FP1005.

  4. The microwave holography system for the Sardinia Radio Telescope

    NASA Astrophysics Data System (ADS)

    Serra, G.; Bolli, P.; Busonera, G.; Pisanu, T.; Poppi, S.; Gaudiomonte, F.; Zacchiroli, G.; Roda, J.; Morsiani, M.; López-Pérez, J. A.

    2012-09-01

    Microwave holography is a well-established technique for mapping surface errors of large reflector antennas, particularly those designed to operate at high frequencies. We present here a holography system based on the interferometric method for mapping the primary reflector surface of the Sardinia Radio Telescope (SRT). SRT is a new 64-m-diameter antenna located in Sardinia, Italy, equipped with an active surface and designed to operate up to 115 GHz. The system consists mainly of two radio frequency low-noise coherent channels, designed to receive Ku-band digital TV signals from geostationary satellites. Two commercial prime focus low-noise block converters are installed on the radio telescope under test and on a small reference antenna, respectively. Then the signals are amplified, filtered and downconverted to baseband. An innovative digital back-end based on FPGA technology has been implemented to digitize two 5 MHz-band signals and calculate their cross-correlation in real-time. This is carried out by using a 16-bit resolution ADCs and a FPGA reaching very large amplitude dynamic range and reducing post-processing time. The final holography data analysis is performed by CLIC data reduction software developed within the Institut de Radioastronomie Millimétrique (IRAM, Grenoble, France). The system was successfully tested during several holography measurement campaigns, recently performed at the Medicina 32-m radio telescope. Two 65-by-65 maps, using an on-the-fly raster scan with on-source phase calibration, were performed pointing the radio telescope at 38 degrees elevation towards EUTELSAT 7A satellite. The high SNR (greater than 60 dB) and the good phase stability led to get an accuracy on the surface error maps better than 150 μm RMS.

  5. Ultra-realistic imaging: a new beginning for display holography

    NASA Astrophysics Data System (ADS)

    Bjelkhagen, Hans I.; Brotherton-Ratcliffe, David

    2014-02-01

    Recent improvements in key foundation technologies are set to potentially transform the field of Display Holography. In particular new recording systems, based on recent DPSS and semiconductor lasers combined with novel recording materials and processing, have now demonstrated full-color analogue holograms of both lower noise and higher spectral accuracy. Progress in illumination technology is leading to a further major reduction in display noise and to a significant increase of the clear image depth and brightness of such holograms. So too, recent progress in 1-step Direct-Write Digital Holography (DWDH) now opens the way to the creation of High Virtual Volume Displays (HVV) - large format full-parallax DWDH reflection holograms having fundamentally larger clear image depths. In a certain fashion HVV displays can be thought of as providing a high quality full-color digital equivalent to the large-format laser-illuminated transmission holograms of the sixties and seventies. Back then, the advent of such holograms led to much optimism for display holography in the market. However, problems with laser illumination, their monochromatic analogue nature and image noise are well cited as being responsible for their failure in reality. Is there reason for believing that the latest technology improvements will make the mark this time around? This paper argues that indeed there is.

  6. Holography In An Industrial Environment

    NASA Astrophysics Data System (ADS)

    Parker, R. J.; Jones, D. G.

    1988-01-01

    Holography has recently achieved new importance as a means of providing the data necessary for the development and validation of computer models. Increasingly, design work is being performed through the application of complicated three-dimensional models to gas flow prediction and the use of finite-element models for investigation of mechanical behaviour and stress. This paper reviews the many applications of holography that have been developed at Rolls-Royce over the past 20 years. These cover all of the major fields within holographic interferometry; flow visualisation, nondestructive testing, vibration analysis, and holographic contouring. Of equal interest with the techniques themselves are the problems encountered and overcome in applying these techniques in a demanding and at times hostile industrial environment away from the laboratory. The extensive use of high-power pulsed lasers has greatly facilitated this move and has allowed holography to be used routinely in normal component test areas.

  7. Electron Holography: phases matter.

    PubMed

    Lichte, Hannes

    2013-06-01

    Essentially, all optics is wave optics, be it with light, X-rays, neutrons or electrons. The information transfer from the object to the image can only be understood in terms of waves given by amplitude and phase. However, phases are difficult to measure: for slowly oscillating waves such as sound or low-frequency electromagnetic waves, phases can be measured directly; for high frequencies this has to be done by heterodyne detection, i.e. superposition with a reference and averaging over time. In optics, this is called interferometry. Because interference is mostly very difficult to achieve, phases have often been considered 'hidden variables' seemingly pulling the strings from backstage, only visible by their action on the image intensity. This was almost the case in conventional Electron Microscopy with the phase differences introduced by an object. However, in the face of the urgent questions from solid state physics and materials science, these phases have to be determined precisely, because they encode the most dominant object properties, such as charge distributions and electromagnetic fields. After more than six decades of very patient advancement, electron interferometry and holography offer unprecedented analytical facilities down to an atomic scale. Akira Tonomura has prominently contributed to the present state. PMID:23620338

  8. Single-exposure color digital holography

    NASA Astrophysics Data System (ADS)

    Feng, Shaotong; Wang, Yanhui; Zhu, Zhuqing; Nie, Shouping

    2010-11-01

    In this paper, we report a method for color image reconstruction by recording only one single multi-wavelength hologram. In the recording process, three lasers of different wavelengths emitting in the red, green and blue regions are used for illuminating on the object and the object diffraction fields will arrive at the hologram plane simultaneously. Three reference beams with different spatial angles will interfere with the corresponding object diffraction fields on the hologram plane, respectively. Finally, a series of sub-holograms incoherently overlapped on the CCD to be recorded as a multi-wavelength hologram. Angular division multiplexing is employed to reference beams so that the spatial spectra of the multiple recordings will be separated in the Fourier plane. In the reconstruction process, the multi-wavelength hologram will be Fourier transformed into its Fourier plane, where the spatial spectra of different wavelengths are separated and can be easily extracted by employing frequency filtering. The extracted spectra are used to reconstruct the corresponding monochromatic complex amplitudes, which will be synthesized to reconstruct the color image. For singleexposure recording technique, it is convenient for applications on the real-time image processing fields. However, the quality of the reconstructed images is affected by speckle noise. How to improve the quality of the images needs for further research.

  9. Content metamorphosis in synthetic holography

    NASA Astrophysics Data System (ADS)

    Desbiens, Jacques

    2013-02-01

    A synthetic hologram is an optical system made of hundreds of images amalgamated in a structure of holographic cells. Each of these images represents a point of view on a three-dimensional space which makes us consider synthetic holography as a multiple points of view perspective system. In the composition of a computer graphics scene for a synthetic hologram, the field of view of the holographic image can be divided into several viewing zones. We can attribute these divisions to any object or image feature independently and operate different transformations on image content. In computer generated holography, we tend to consider content variations as a continuous animation much like a short movie. However, by composing sequential variations of image features in relation with spatial divisions, we can build new narrative forms distinct from linear cinematographic narration. When observers move freely and change their viewing positions, they travel from one field of view division to another. In synthetic holography, metamorphoses of image content are within the observer's path. In all imaging Medias, the transformation of image features in synchronisation with the observer's position is a rare occurrence. However, this is a predominant characteristic of synthetic holography. This paper describes some of my experimental works in the development of metamorphic holographic images.

  10. Rainbow holography and its applications

    NASA Astrophysics Data System (ADS)

    Vlasov, N. G.; Ivanov, Vladimir S.

    1993-09-01

    The general equations of the rainbow holography are deduced. Their analysis makes it possible to offer different methods of the rainbow holographic images production. A new way of using the rainbow holograms as optical elements for effective color illuminating of transparent, specular, and polished objects is proposed. Application fields are the advertising industry, shop windows design, etc.

  11. Image magnification in lensless holographic projection using double-sampling Fresnel diffraction.

    PubMed

    Qu, Weidong; Gu, Huarong; Zhang, Hao; Tan, Qiaofeng

    2015-12-01

    Since the diffraction angle is limited by the spatial resolution of the spatial light modulator (SLM), the size of the optical image of the lensless holographic projection with a SLM is very small. Using a divergent spherical beam to illuminate a SLM is an effective method to physically increase the projection angle; nevertheless, the sampling ranges of the existing Fresnel diffraction algorithms with fast Fourier transform keep unchanged. In this paper, a double-sampling Fresnel diffraction algorithm to enlarge the sampling range is proposed when using a divergent spherical beam to illuminate a SLM, and the magnification of the optical image is realized in lensless holographic projection. The hologram can be easily optimized by the Gerschberg-Saxton algorithm. Simulation and experimental results with enlarged optical image are presented successfully. PMID:26836654

  12. Lensless imaging using broadband X-ray sources

    NASA Astrophysics Data System (ADS)

    Abbey, Brian; Whitehead, Lachlan W.; Quiney, Harry M.; Vine, David J.; Cadenazzi, Guido A.; Henderson, Clare A.; Nugent, Keith A.; Balaur, Eugeniu; Putkunz, Corey T.; Peele, Andrew G.; Williams, G. J.; McNulty, I.

    2011-07-01

    High-resolution X-ray imaging techniques using optical elements such as zone plates are widely used for viewing the internal structure of samples in exquisite detail. The resolution attainable is ultimately limited by the manufacturing tolerances for the optics. Combining ideas from crystallography and holography, this limit may be surpassed by the method of coherent diffractive imaging (CDI). Although CDI shows particular promise in applications involving X-ray free-electron lasers, it is also emerging as an important new technique for imaging at third-generation synchrotrons. The limited coherent output of these sources, however, is a significant barrier to obtaining shorter exposure times. A fundamental assumption of coherent diffractive imaging is that the incident light is well-approximated by a single optical frequency. In this Letter, we demonstrate the first experimental realization of `polyCDI', using a broadband source to achieve a factor of 60 reduction in the exposure time over quasi-monochromatic coherent diffractive imaging.

  13. Industrial holography: The Rolls-Royce experience

    NASA Astrophysics Data System (ADS)

    Parker, R. J.; Jones, D. G.

    1986-07-01

    Holographic interferometry applications to flow visualization, nondestructive testing, vibration analysis, and holographic contouring are described. Problems encountered and overcome in applying these techniques in a demanding, at times hostile, environment away from the laboratory are discussed. Holography for routine vibration analysis; pulsed holography of large, vibrating assemblies; holographic vibrometry on rotating components; holographic flow visualization in wind tunnels; and pulsed holography in rotating, transonic flow are illustrated.

  14. Ultra-realistic 3-D imaging based on colour holography

    NASA Astrophysics Data System (ADS)

    Bjelkhagen, H. I.

    2013-02-01

    A review of recent progress in colour holography is provided with new applications. Colour holography recording techniques in silver-halide emulsions are discussed. Both analogue, mainly Denisyuk colour holograms, and digitally-printed colour holograms are described and their recent improvements. An alternative to silver-halide materials are the panchromatic photopolymer materials such as the DuPont and Bayer photopolymers which are covered. The light sources used to illuminate the recorded holograms are very important to obtain ultra-realistic 3-D images. In particular the new light sources based on RGB LEDs are described. They show improved image quality over today's commonly used halogen lights. Recent work in colour holography by holographers and companies in different countries around the world are included. To record and display ultra-realistic 3-D images with perfect colour rendering are highly dependent on the correct recording technique using the optimal recording laser wavelengths, the availability of improved panchromatic recording materials and combined with new display light sources.

  15. Integrating microfluidics and lensless imaging for point-of-care testing.

    PubMed

    Moon, Sangjun; Keles, Hasan Onur; Ozcan, Aydogan; Khademhosseini, Ali; Haeggstrom, Edward; Kuritzkes, Daniel; Demirci, Utkan

    2009-07-15

    We demonstrate an integrated platform that merges a microfluidic chip with lensless imaging to target CD4(+) T-lymphocyte counts for HIV point-of-care testing at resource-limited settings. The chips were designed and fabricated simply with a laser cutter without using expensive cleanroom equipment. To capture CD4(+) T-lymphocytes from blood, anti-CD4 antibody was immobilized on only one side of the microfluidic chip. These captured cells were detected through an optically clear chip using a charge coupled device (CCD) sensor by lensless shadow imaging techniques. Gray scale image of the captured cells in a 24 mm x 4 mm x 50 microm microfluidic chip was obtained by the lensless imaging platform. The automatic cell counting software enumerated the captured cells in 3s. Captured cells were also imaged with a fluorescence microscope and manually counted to characterize functionality of the integrated platform. The integrated platform achieved 70.2+/-6.5% capture efficiency, 88.8+/-5.4% capture specificity for CD4(+) T-lymphocytes, 96+/-1.6% CCD efficiency, and 83.5+/-2.4% overall platform performance (n=9 devices) compared to the gold standard, i.e. flow cytometry count. The integrated system gives a CD4 count from blood within 10 min. The integrated platform points a promising direction for point-of-care testing (POCT) to rapidly capture, image and count subpopulations of cells from blood samples in an automated matter. PMID:19467854

  16. 'Micromanaging de Sitter holography'

    SciTech Connect

    Dong, Xi; Horn, Bart; Silverstein, Eva; Torroba, Gonzalo; /SLAC /Stanford U., Phys. Dept. /Santa Barbara, KITP

    2010-08-26

    We develop tools to engineer de Sitter vacua with semi-holographic duals, using elliptic fibrations and orientifolds to uplift Freund-Rubin compactifications with CFT duals. The dual brane construction is compact and constitutes a microscopic realization of the dS/dS correspondence, realizing d-dimensional de Sitter space as a warped compactification down to (d-1)-dimensional de Sitter gravity coupled to a pair of large-N matter sectors. This provides a parametric microscopic interpretation of the Gibbons-Hawking entropy. We illustrate these ideas with an explicit class of examples in three dimensions, and describe ongoing work on four-dimensional constructions. The Gibbons-Hawking entropy of the de Sitter horizon [1] invites a microscopic interpretation and a holographic formulation of inflating spacetimes. Much progress was made in the analogous problem in black hole physics using special black holes in string theory whose microstates could be reliably counted, such as those analyzed in [2,3]; this led to the AdS/CFT correspondence [4]. In contrast, a microscopic understanding of the entropy of de Sitter space is more difficult for several reasons including its potential dynamical connections to other backgrounds (metastability), the absence of a non-fluctuating timelike boundary, and the absence of supersymmetry. In this paper, we develop a class of de Sitter constructions in string theory, built up from AdS/CFT dual pairs along the lines of [5], which are simple enough to provide a microscopic accounting of the parametric scaling of the Gibbons-Hawking entropy. These models realize microscopically a semi-holographic description of metastable de Sitter space which had been derived macroscopically in [6]. It would also be interesting to connect this to other approaches to de Sitter holography such as [7, 8] and to other manifestations of the de Sitter entropy such as [9]. The construction is somewhat analogous to neutral black branes analyzed in [11]. We will

  17. Spatially-Heterodyned Holography

    DOEpatents

    Thomas, Clarence E [Knoxville, TN; Hanson, Gregory R [Clinton, TN

    2006-02-21

    A method of recording a spatially low-frequency heterodyne hologram, including spatially heterodyne fringes for Fourier analysis, includes: splitting a laser beam into a reference beam and an object beam; interacting the object beam with an object; focusing the reference beam and the object beam at a focal plane of a digital recorder to form a spatially low-frequency heterodyne hologram including spatially heterodyne fringes for Fourier analysis; digital recording the spatially low-frequency heterodyne hologram; Fourier transforming axes of the recorded spatially low-frequency heterodyne hologram including spatially heterodyne fringes in Fourier space to sit on top of a heterodyne carrier frequency defined by an angle between the reference beam and the object beam; cutting off signals around an origin; and performing an inverse Fourier transform.

  18. Holography: childrens' window to relativity

    NASA Astrophysics Data System (ADS)

    MacShane, James E.

    1995-02-01

    This paper is concerned with the development of the concept of natural education. Psychology has discovered that all humans learn intuitively the cultural concepts of time from birth to eight and one-half to ten years of age. Einstein showed us that this must also be the natural time for the development of spatial concepts. The importance of this has been dramatized for me in the past eight years that I have been developing the Laser Arts and Holography Programs and Workshops. I have worked with over 100,000 students kindergarten through eighth grade. I have worked with 175 students age 8 to 10 in three hour and one half workshops specifically on the development of time and space concepts. The concepts developed are based upon the vast amount of psychological evidence related to the natural development of time and space understandings, Dr. Nils Abramson's 'Light in Flight' and subsequent work on the clarification of relativity through holography, and Maria Montessori's method of Scientific Education. The paper also demonstrates the natural method of teaching science to younger students is to teach scientifically. All of the research which has been done in the past 100 years has been used by the educational institutions to try to improve the system. What has not been done is changing the system to how humans learn. Because of the perceived hi-tech nature of the program I am able to dramatize the potential. An outline for a holography curriculum kindergarten through eighth grade is included.

  19. HOLONET: a network for training holography

    NASA Astrophysics Data System (ADS)

    Pombo, Pedro; Santos, Emanuel

    2014-07-01

    Holography is an optics technique based on wave physics and lasers with several applications at our day life. The production of holograms involves experimental work based on hands-on activities and creativity. All these elements can contribute to the promotion of experimental teaching of optics and training on holography. The hologram itself acting as a final result from a long process of research and study can enable the engagement of high school students on physics and promote the stimulus on optics learning. Taking these assumptions into account a network of schools working on holography was built involving thirty schools from all country. Holography systems were developed and several hands-on activities were constructed. During last sixteen years students are working on laser optics and holography producing different kinds of holograms. This study presents all holography labs implemented at schools and it will analyzed the holography systems and materials developed for students. Training strategy will be discussed and holograms obtained by students will be presented. Results obtained show us that holography can be implemented as a strategy for promoting the learning of optics and it is a particular way to involve students on experimental work and lab research. Results obtained during this study will be presented in detail and analyzed with focus on students performance. Educational results, teachers training, prizes and other positive outcomes will be discussed and compared.

  20. Integrating Microfluidics and Lensless Imaging for Point-of-Care Testing

    PubMed Central

    Moon, SangJun; Keles, Hasan Onur; Ozcan, Aydogan; Khademhosseini, Ali; Hæggstrom, Edward; Kuritzkes, Daniel; Demirci, Utkan

    2009-01-01

    We demonstrate an integrated platform that merges a microfluidic chip with lensless imaging to target CD4+ T-lymphocyte counts for HIV point-of-care testing at resource-limited settings. The chips were designed and fabricated simply with a laser cutter without using expensive cleanroom equipment. To capture CD4+ T lymphocytes from blood, anti-CD4 antibody was immobilized on only one side of the microfluidic chip. These captured cells were detected through an optically clear chip using a charge coupled device (CCD) sensor by lensless shadow imaging techniques. Gray scale image of the captured cells in a 24 mm × 4 mm × 50 μm microfluidic chip was obtained by the lensless imaging platform. The automatic cell counting software enumerated the captured cells in three seconds. Captured cells were also imaged with a fluorescence microscope and manually counted to characterize functionality of the integrated platform. The integrated platform achieved 70.2 ± 6.5% capture efficiency, 88.8 ± 5.4% capture specificity for CD4+ T-lymphocytes, 96 ± 1.6% CCD efficiency, and 83.5 ± 2.4% overall platform performance (n = 9 devices) compared to the gold standard, i.e. flow cytometry count. The integrated system gives a CD4 count from blood within 10 minutes. The integrated platform points a promising direction for point-of-care testing (POCT) to rapidly capture, image and count subpopulations of cells from blood samples in an automated matter. PMID:19467854

  1. Lensless focusing with subwavelength resolution by direct synthesis of the angular spectrum

    NASA Astrophysics Data System (ADS)

    Hong, Stanley S.; Horn, Berthold K. P.; Freeman, Dennis M.; Mermelstein, Michael S.

    2006-06-01

    We consider the coherent superposition of unfocused wave fronts for lensless focusing of electromagnetic waves with subwavelength resolution. Near the focal point, intensity distributions generated using the approach approximate those generated using lenses. Far from the focal point, discretization of spatial frequencies results in a trade-off between the number of wave fronts and the accuracy of the approximation. We experimentally demonstrate the feasibility of the approach by generating an approximation of an azimuthally polarized Bessel beam with a focal spot diameter (full width at half maximum intensity) of 0.37λ.

  2. Parallel lensless optical correlator based on two phase-only spatial light modulators.

    PubMed

    Zeng, Xu; Inoue, Takashi; Fukuchi, Norihiro; Bai, Jian

    2011-06-20

    In this paper, we proposed a parallel phase-only lensless optical correlator based on two pieces of Liquid Crystal on Silicon Spatial Light Modulators. Phase Fresnel Lens Array and specialized grating are implemented to realize multi-channel and multiplexed LOC. Experimental results of Chinese characters' recognitions are given as demonstration of proposed technique. High uniformity of processing channels has been verified by autocorrelation process of four same Chinese characters. The technique is programmable and adjustment of optical path could be realized without changing of optical setup. The implementations could be performed on the same configuration as single channel optical correlator without mechanical alternation. PMID:21716500

  3. Artistic Representation with Pulsed Holography

    NASA Astrophysics Data System (ADS)

    Ishii, S.

    2013-02-01

    This thesis describes artistic representation through pulsed holography. One of the prevalent practical problems in making holograms is object movement. Any movement of the object or film, including movement caused by acoustic vibration, has the same fatal results. One way of reducing the chance of movement is by ensuring that the exposure is very quick; using a pulsed laser can fulfill this objective. The attractiveness of using pulsed laser is based on the variety of materials or objects that can be recorded (e.g., liquid material or instantaneous scene of a moving object). One of the most interesting points about pulsed holograms is that some reconstructed images present us with completely different views of the real world. For example, the holographic image of liquid material does not appear fluid; it looks like a piece of hard glass that would produce a sharp sound upon tapping. In everyday life, we are unfamiliar with such an instantaneous scene. On the other hand, soft-textured materials such as a feather or wool differ from liquids when observed through holography. Using a pulsed hologram, we can sense the soft touch of the object or material with the help of realistic three-dimensional (3-D) images. The images allow us to realize the sense of touch in a way that resembles touching real objects. I had the opportunity to use a pulsed ruby laser soon after I started to work in the field of holography in 1979. Since then, I have made pulsed holograms of activities, including pouring water, breaking eggs, blowing soap bubbles, and scattering feathers and popcorn. I have also created holographic art with materials and objects, such as silk fiber, fabric, balloons, glass, flowers, and even the human body. Whenever I create art, I like to present the spectator with a new experience in perception. Therefore, I would like to introduce my experimental artwork through those pulsed holograms.

  4. Aesthetics and representation in holography

    NASA Astrophysics Data System (ADS)

    Kac, Eduardo

    1995-02-01

    Every medium has a code, a set of rules or conventions according to which determined elements are organized into a signifying system. The English language is a code as is perspective in painting and photography. In the first case, the elements are phonemes organized into words and sentences according to a social convention: the syntax of English. In the second case, the elements are dots and lines organized into pictures according to a geometric method. An artist or movement can break the conventions of the medium, as has done Cezanne with painting, Moholy-Nagy with photography and cummings with the English idiom in poetry, and create new elements and rules for combining them. If this is done, the level of predictability (or conventionality) is lowered and unpredictability is increased -- becoming more difficult for the immediate audience to understand it. But once these new rules are learned and the ideas behind them widely understood, the level of unpredictability is lowered and they become new conventions that can be accepted by the audience. Holographic artists exploring the medium -- as opposed to advertisers using holography, who favor a high level of predictability -- are breaking several visual and cultural conventions. As a matter of fact, holography is so new that many questions are left open about the nature of the medium. Therefore, any attempt to clarify the issues raised by holography on a cultural level has a prospective (and not conclusive) tone, concentrating more thoroughly on general points and on the promise of its potentialities than on the records of its historical achievements so far.

  5. Seismic Holography of Solar Activity

    NASA Technical Reports Server (NTRS)

    Lindsey, Charles

    2000-01-01

    The basic goal of the project was to extend holographic seismic imaging techniques developed under a previous NASA contract, and to incorporate phase diagnostics. Phase-sensitive imaging gives us a powerful probe of local thermal and Doppler perturbations in active region subphotospheres, allowing us to map thermal structure and flows associated with "acoustic moats" and "acoustic glories". These remarkable features were discovered during our work, by applying simple acoustic power holography to active regions. Included in the original project statement was an effort to obtain the first seismic images of active regions on the Sun's far surface.

  6. Damage Detection Using Holography and Interferometry

    NASA Technical Reports Server (NTRS)

    Decker, Arthur J.

    2003-01-01

    This paper reviews classical approaches to damage detection using laser holography and interferometry. The paper then details the modern uses of electronic holography and neural-net-processed characteristic patterns to detect structural damage. The design of the neural networks and the preparation of the training sets are discussed. The use of a technique to optimize the training sets, called folding, is explained. Then a training procedure is detailed that uses the holography-measured vibration modes of the undamaged structures to impart damage-detection sensitivity to the neural networks. The inspections of an optical strain gauge mounting plate and an International Space Station cold plate are presented as examples.

  7. Experimental verification of transient nonlinear acoustical holography.

    PubMed

    Jing, Yun; Cannata, Jonathan; Wang, Tianren

    2013-05-01

    This paper presents an experimental study on nonlinear transient acoustical holography. The validity and effectiveness of a recently proposed nonlinear transient acoustical holography algorithm is evaluated in the presence of noise. The acoustic field measured on a post-focal plane of a high-intensity focused transducer is backward projected to reconstruct the pressure distributions on the focal and a pre-focal plane, which are shown to be in good agreement with the measurement. In contrast, the conventional linear holography produces erroneous results in this case where the nonlinearity involved is strong. Forward acoustic field projection was also carried out to further verify the algorithm. PMID:23654362

  8. Experimental verification of transient nonlinear acoustical holography.

    PubMed

    Jing, Yun; Cannata, Jonathan; Wang, Tianren

    2013-05-01

    This paper presents an experimental study on nonlinear transient acoustical holography. The validity and effectiveness of a recently proposed nonlinear transient acoustical holography algorithm is evaluated in the presence of noise. The acoustic field measured on a post-focal plane of a high-intensity focused transducer is backward projected to reconstruct the pressure distributions on the focal and a pre-focal plane, which are shown to be in good agreement with the measurement. In contrast, the conventional linear holography produces erroneous results in this case where the nonlinearity involved is strong. Forward acoustic field projection was also carried out to further verify the algorithm.

  9. THz holography in reflection using a high resolution microbolometer array.

    PubMed

    Zolliker, Peter; Hack, Erwin

    2015-05-01

    We demonstrate a digital holographic setup for Terahertz imaging of surfaces in reflection. The set-up is based on a high-power continuous wave (CW) THz laser and a high-resolution (640 × 480 pixel) bolometer detector array. Wave propagation to non-parallel planes is used to reconstruct the object surface that is rotated relative to the detector plane. In addition we implement synthetic aperture methods for resolution enhancement and compare Fourier transform phase retrieval to phase stepping methods. A lateral resolution of 200 μm and a relative phase sensitivity of about 0.4 rad corresponding to a depth resolution of 6 μm are estimated from reconstructed images of two specially prepared test targets, respectively. We highlight the use of digital THz holography for surface profilometry as well as its potential for video-rate imaging.

  10. Present State Iof Holography In Japan

    NASA Astrophysics Data System (ADS)

    Suzuki, Masane; Hayashi, Yuzo; Yamamoto, Y.

    1983-07-01

    In 1948,Dr. Dennis Gabor introduced the theory of holography as "the optical recording of the object wave formed by the resulting interference pattern of two mutually coherent, component light beams." Through the studies of enumerous practical applications, the theory of holography was further advanced to be used in conjunction with the laser beam to better serve a more practical minded industry. Such developments were introduced and engineered by Dr. Emmett Leith and Dr. Juris Upatnieks in 1962.

  11. Soft x-ray holography and microscopy of biological cells

    NASA Astrophysics Data System (ADS)

    Chen, Jianwen; Gao, Hongyi; Xie, Honglan; Li, Ruxin; Xu, Zhizhan

    2003-10-01

    Some experimental results on soft X-ray microscopy and holography imaging of biological specimens are presented in the paper. As we know, due to diffraction effects, there exists a resolution limit determined by wavelength λ and numerical aperture NA in conventional optical microscopy. In order to improve resolution, the num erical aperture should be made as large as possible and the wavelength as short as possible. Owing to the shorter wavelength, X-rays provide the potential of higher resolution in X-ray microscopy, holography image and allow for exam ination the interior structures of thicker specimens. In the experiments, we used synchrotron radiation source in Hefei as light source. Soft X-rays come from a bending magnet in 800 M eV electron storage ring with characteristic wavelength of 2.4 nm. The continuous X-ray spectrums are monochromatized by a zone-plate and a pinhole with 300 m diameter. The experimental set-up is typical contact microscopic system, its main advantage is simplicity and no special optical element is needed. The specimens used in the experiments of microscopic imaging are the colibacillus, the gingko vascular hundle and the fritillaries ovary karyon. The specimen for holographic imaging is the spider filam ents. The basic structures of plant cells such as the cell walls, the cytoplasm and the karyon especially the joint structures between the cells are observed clearly. An experimental study on a thick biological specimen that is a whole sporule w ith the thickness of about 30 μm is performed. In the holographic experiments, the experimental setup is typical Gabor in-line holography. The specimen is placed in line with X-ray source, which provides both the reference w aves and specimen illum ination. The specimen is some spider filament, which adhere to a Si3N4 film. The recording medium is PM M A, which is placed at recording distance of about 400 μm from the specimen. The hologram s were reconstructed by digital method with 300 nm

  12. Ultra wide band 3-D cross section (RCS) holography

    SciTech Connect

    Collins, H.D.; Hall, T.E.

    1992-07-01

    Ultra wide band impulse holography is an exciting new concept for predictive radar cross section (RCS) evaluation employing near-field measurements. Reconstruction of the near-field hologram data maps the target`s scattering areas, and uniquely identifies the ``hot spot`` locations on the target. In addition, the target and calibration sphere`s plane wave angular spectrums are computed (via digital algorithm) and used to generate the target`s far-field RCS values in three dimensions for each frequency component in the impulse. Thin and thick targets are defined in terms of their near-field amplitude variations in range. Range gating and computer holographic techniques are applied to correct these variations. Preliminary experimental results on various targets verify the concept of RCS holography. The unique 3-D presentation (i.e., typically containing 524,288 RCS values for a 1024 {times} 512 sampled aperture for every frequency component) illustrates the efficacy of target recognition in terms of its far-field plane wave angular spectrum image. RCS images can then be viewed at different angles for target recognition, etc.

  13. Ultra wide band 3-D cross section (RCS) holography

    SciTech Connect

    Collins, H.D.; Hall, T.E.

    1992-07-01

    Ultra wide band impulse holography is an exciting new concept for predictive radar cross section (RCS) evaluation employing near-field measurements. Reconstruction of the near-field hologram data maps the target's scattering areas, and uniquely identifies the hot spot'' locations on the target. In addition, the target and calibration sphere's plane wave angular spectrums are computed (via digital algorithm) and used to generate the target's far-field RCS values in three dimensions for each frequency component in the impulse. Thin and thick targets are defined in terms of their near-field amplitude variations in range. Range gating and computer holographic techniques are applied to correct these variations. Preliminary experimental results on various targets verify the concept of RCS holography. The unique 3-D presentation (i.e., typically containing 524,288 RCS values for a 1024 {times} 512 sampled aperture for every frequency component) illustrates the efficacy of target recognition in terms of its far-field plane wave angular spectrum image. RCS images can then be viewed at different angles for target recognition, etc.

  14. Acoustic emission linear pulse holography

    DOEpatents

    Collins, H.D.; Busse, L.J.; Lemon, D.K.

    1983-10-25

    This device relates to the concept of and means for performing Acoustic Emission Linear Pulse Holography, which combines the advantages of linear holographic imaging and Acoustic Emission into a single non-destructive inspection system. This unique system produces a chronological, linear holographic image of a flaw by utilizing the acoustic energy emitted during crack growth. The innovation is the concept of utilizing the crack-generated acoustic emission energy to generate a chronological series of images of a growing crack by applying linear, pulse holographic processing to the acoustic emission data. The process is implemented by placing on a structure an array of piezoelectric sensors (typically 16 or 32 of them) near the defect location. A reference sensor is placed between the defect and the array.

  15. Optimal quantisation applied to digital holographic data

    NASA Astrophysics Data System (ADS)

    Shortt, Alison E.; Naughton, Thomas J.; Javidi, Bahram

    2005-06-01

    Digital holography is an inherently three-dimensional (3D) technique for the capture of real-world objects. Many existing 3D imaging and processing techniques are based on the explicit combination of several 2D perspectives (or light stripes, etc.) through digital image processing. The advantage of recording a hologram is that multiple 2D perspectives can be optically combined in parallel, and in a constant number of steps independent of the hologram size. Although holography and its capabilities have been known for many decades, it is only very recently that digital holography has been practically investigated due to the recent development of megapixel digital sensors with sufficient spatial resolution and dynamic range. The applications of digital holography could include 3D television, virtual reality, and medical imaging. If these applications are realised, compression standards will have to be defined. We outline the techniques that have been proposed to date for the compression of digital hologram data and show that they are comparable to the performance of what in communication theory is known as optimal signal quantisation. We adapt the optimal signal quantisation technique to complex-valued 2D signals. The technique relies on knowledge of the histograms of real and imaginary values in the digital holograms. Our digital holograms of 3D objects are captured using phase-shift interferometry.

  16. Holography and coherent diffraction with low-energy electrons: A route towards structural biology at the single molecule level.

    PubMed

    Latychevskaia, Tatiana; Longchamp, Jean-Nicolas; Escher, Conrad; Fink, Hans-Werner

    2015-12-01

    The current state of the art in structural biology is led by NMR, X-ray crystallography and TEM investigations. These powerful tools however all rely on averaging over a large ensemble of molecules. Here, we present an alternative concept aiming at structural analysis at the single molecule level. We show that by combining electron holography and coherent diffraction imaging estimations concerning the phase of the scattered wave become needless as the phase information is extracted from the data directly and unambiguously. Performed with low-energy electrons the resolution of this lens-less microscope is just limited by the De Broglie wavelength of the electron wave and the numerical aperture, given by detector geometry. In imaging freestanding graphene, a resolution of 2Å has been achieved revealing the 660.000 unit cells of the graphene sheet from a single data set. Once applied to individual biomolecules the method shall ultimately allow for non-destructive imaging and imports the potential to distinguish between different conformations of proteins with atomic resolution.

  17. Lensless optical data hiding system based on phase encoding algorithm in the Fresnel domain.

    PubMed

    Chen, Yen-Yu; Wang, Jian-Hong; Lin, Cheng-Chung; Hwang, Hone-Ene

    2013-07-20

    A novel and efficient algorithm based on a modified Gerchberg-Saxton algorithm (MGSA) in the Fresnel domain is presented, together with mathematical derivation, and two pure phase-only masks (POMs) are generated. The algorithm's application to data hiding is demonstrated by a simulation procedure, in which a hidden image/logo is encoded into phase forms. A hidden image/logo can be extracted by the proposed high-performance lensless optical data-hiding system. The reconstructed image shows good quality and the errors are close to zero. In addition, the robustness of our data-hiding technique is illustrated by simulation results. The position coordinates of the POMs as well as the wavelength are used as secure keys that can ensure sufficient information security and robustness. The main advantages of this proposed watermarking system are that it uses fewer iterative processes to produce the masks, and the image-hiding scheme is straightforward.

  18. A 72 × 60 Angle-Sensitive SPAD Imaging Array for Lens-less FLIM

    PubMed Central

    Lee, Changhyuk; Johnson, Ben; Jung, TaeSung; Molnar, Alyosha

    2016-01-01

    We present a 72 × 60, angle-sensitive single photon avalanche diode (A-SPAD) array for lens-less 3D fluorescence lifetime imaging. An A-SPAD pixel consists of (1) a SPAD to provide precise photon arrival time where a time-resolved operation is utilized to avoid stimulus-induced saturation, and (2) integrated diffraction gratings on top of the SPAD to extract incident angles of the incoming light. The combination enables mapping of fluorescent sources with different lifetimes in 3D space down to micrometer scale. Futhermore, the chip presented herein integrates pixel-level counters to reduce output data-rate and to enable a precise timing control. The array is implemented in standard 180 nm complementary metal-oxide-semiconductor (CMOS) technology and characterized without any post-processing. PMID:27598170

  19. A 72 × 60 Angle-Sensitive SPAD Imaging Array for Lens-less FLIM.

    PubMed

    Lee, Changhyuk; Johnson, Ben; Jung, TaeSung; Molnar, Alyosha

    2016-01-01

    We present a 72 × 60, angle-sensitive single photon avalanche diode (A-SPAD) array for lens-less 3D fluorescence lifetime imaging. An A-SPAD pixel consists of (1) a SPAD to provide precise photon arrival time where a time-resolved operation is utilized to avoid stimulus-induced saturation, and (2) integrated diffraction gratings on top of the SPAD to extract incident angles of the incoming light. The combination enables mapping of fluorescent sources with different lifetimes in 3D space down to micrometer scale. Futhermore, the chip presented herein integrates pixel-level counters to reduce output data-rate and to enable a precise timing control. The array is implemented in standard 180 nm complementary metal-oxide-semiconductor (CMOS) technology and characterized without any post-processing. PMID:27598170

  20. A 72 × 60 Angle-Sensitive SPAD Imaging Array for Lens-less FLIM.

    PubMed

    Lee, Changhyuk; Johnson, Ben; Jung, TaeSung; Molnar, Alyosha

    2016-01-01

    We present a 72 × 60, angle-sensitive single photon avalanche diode (A-SPAD) array for lens-less 3D fluorescence lifetime imaging. An A-SPAD pixel consists of (1) a SPAD to provide precise photon arrival time where a time-resolved operation is utilized to avoid stimulus-induced saturation, and (2) integrated diffraction gratings on top of the SPAD to extract incident angles of the incoming light. The combination enables mapping of fluorescent sources with different lifetimes in 3D space down to micrometer scale. Futhermore, the chip presented herein integrates pixel-level counters to reduce output data-rate and to enable a precise timing control. The array is implemented in standard 180 nm complementary metal-oxide-semiconductor (CMOS) technology and characterized without any post-processing.

  1. Lensless transport-of-intensity phase microscopy and tomography with a color LED matrix

    NASA Astrophysics Data System (ADS)

    Zuo, Chao; Sun, Jiasong; Zhang, Jialin; Hu, Yan; Chen, Qian

    2015-07-01

    We demonstrate lens-less quantitative phase microscopy and diffraction tomography based on a compact on-chip platform, using only a CMOS image sensor and a programmable color LED array. Based on multi-wavelength transport-of- intensity phase retrieval and multi-angle illumination diffraction tomography, this platform offers high quality, depth resolved images with a lateral resolution of ˜3.7μm and an axial resolution of ˜5μm, over wide large imaging FOV of 24mm2. The resolution and FOV can be further improved by using a larger image sensors with small pixels straightforwardly. This compact, low-cost, robust, portable platform with a decent imaging performance may offer a cost-effective tool for telemedicine needs, or for reducing health care costs for point-of-care diagnostics in resource-limited environments.

  2. Recent developments on holography in China

    NASA Astrophysics Data System (ADS)

    Hsu, Dahsiung; Jiao, Jiangzhong; Tao, Huiying; Long, Pin

    1991-02-01

    Since the 1985 Lake Forest International Conference on display holography, USA) more developments have been made on holographic applications in China among which the important events and progress were: the International Conference on holography applications em bossing holography new holographic optical elements and equipments etc. . 1. INTERNATIONAL CONFEPENCE ON GRAPHY APPLICATIONS'' ( I CHA ''86. BEIJING) More than 280 holographers from 18 countries gathered in the Science Hall in Beijing from July 2 to for the International Conference on holography applications''86. The conference was sponsor ed by the Chinese OPtical Society the Chinese Theoretical Applied Mechanics Society and cosponsored by the Society of Photooptical Instrumentation Engineers the European Photonic Association in cooperation with the China Association for Science And Technology. It was chaired by Prof. Wang Daheng vice president of the China Association for Science Technology and president of the Chinese Optical Society cochaired by DP. H. J. Caulfield of the Univ. of Alabama Dr. G. von Bally of the Munster Univ. in West Germany and Dr. J. Tsujiuchi of the Tokyo Institute of Technology. SPIE Vol. 1238 Three-Dimensional Holography: Science Culture Education (1989) / 13

  3. Improving the resolution in phase-shifting Gabor holography by CCD shift

    NASA Astrophysics Data System (ADS)

    Granero, L.; Micó, V.; Zalevsky, Z.; García, J.; Javidi, B.

    2015-05-01

    Holography dates back to the year when Dennis Gabor reported on a method to avoid spherical aberration and to improve image quality in electron microscopy. Gabor's two-step holographic method was pioneer but suffered from three major drawbacks: the reconstructed image is affected by coherent noise, the twin image problem of holography that also affects the final image quality, and a restricted sample range (weak diffraction assumption) for preserving the holographic behavior of the method. Nowadays, most of those drawbacks have been overcome and new capabilities have been added due to the replacement of the classical recording media (photographic plate) by digital sensors (CCD and CMOS cameras). But in the Gabor' regime, holography is restricted to weak diffraction assumptions because otherwise, diffraction prevents an accurate recovery of the object's complex wavefront. In this contribution, we present an experimental approach to overcome such limitation and improve final image resolution. We use the phase-shifting Gabor configuration while the CCD camera is shifted to different off-axis positions in order to capture a bigger portion of the diffracted wavefront. Thus, once the whole image set is recorded and digitally processed for each camera's position, we merge the resulting band-pass images into one image by assembling a synthetic aperture. Finally, a superresolved image is recovered by Fourier transformation of the information contained in the generated synthetic aperture. Experimental results are provided using a USAF resolution test target and validating our concepts for a gain in resolution of close to 2.

  4. Spin and wavelength multiplexed nonlinear metasurface holography

    NASA Astrophysics Data System (ADS)

    Ye, Weimin; Zeuner, Franziska; Li, Xin; Reineke, Bernhard; He, Shan; Qiu, Cheng-Wei; Liu, Juan; Wang, Yongtian; Zhang, Shuang; Zentgraf, Thomas

    2016-06-01

    Metasurfaces, as the ultrathin version of metamaterials, have caught growing attention due to their superior capability in controlling the phase, amplitude and polarization states of light. Among various types of metasurfaces, geometric metasurface that encodes a geometric or Pancharatnam-Berry phase into the orientation angle of the constituent meta-atoms has shown great potential in controlling light in both linear and nonlinear optical regimes. The robust and dispersionless nature of the geometric phase simplifies the wave manipulation tremendously. Benefitting from the continuous phase control, metasurface holography has exhibited advantages over conventional depth controlled holography with discretized phase levels. Here we report on spin and wavelength multiplexed nonlinear metasurface holography, which allows construction of multiple target holographic images carried independently by the fundamental and harmonic generation waves of different spins. The nonlinear holograms provide independent, nondispersive and crosstalk-free post-selective channels for holographic multiplexing and multidimensional optical data storages, anti-counterfeiting, and optical encryption.

  5. Spin and wavelength multiplexed nonlinear metasurface holography

    PubMed Central

    Ye, Weimin; Zeuner, Franziska; Li, Xin; Reineke, Bernhard; He, Shan; Qiu, Cheng-Wei; Liu, Juan; Wang, Yongtian; Zhang, Shuang; Zentgraf, Thomas

    2016-01-01

    Metasurfaces, as the ultrathin version of metamaterials, have caught growing attention due to their superior capability in controlling the phase, amplitude and polarization states of light. Among various types of metasurfaces, geometric metasurface that encodes a geometric or Pancharatnam–Berry phase into the orientation angle of the constituent meta-atoms has shown great potential in controlling light in both linear and nonlinear optical regimes. The robust and dispersionless nature of the geometric phase simplifies the wave manipulation tremendously. Benefitting from the continuous phase control, metasurface holography has exhibited advantages over conventional depth controlled holography with discretized phase levels. Here we report on spin and wavelength multiplexed nonlinear metasurface holography, which allows construction of multiple target holographic images carried independently by the fundamental and harmonic generation waves of different spins. The nonlinear holograms provide independent, nondispersive and crosstalk-free post-selective channels for holographic multiplexing and multidimensional optical data storages, anti-counterfeiting, and optical encryption. PMID:27306147

  6. Spin and wavelength multiplexed nonlinear metasurface holography.

    PubMed

    Ye, Weimin; Zeuner, Franziska; Li, Xin; Reineke, Bernhard; He, Shan; Qiu, Cheng-Wei; Liu, Juan; Wang, Yongtian; Zhang, Shuang; Zentgraf, Thomas

    2016-01-01

    Metasurfaces, as the ultrathin version of metamaterials, have caught growing attention due to their superior capability in controlling the phase, amplitude and polarization states of light. Among various types of metasurfaces, geometric metasurface that encodes a geometric or Pancharatnam-Berry phase into the orientation angle of the constituent meta-atoms has shown great potential in controlling light in both linear and nonlinear optical regimes. The robust and dispersionless nature of the geometric phase simplifies the wave manipulation tremendously. Benefitting from the continuous phase control, metasurface holography has exhibited advantages over conventional depth controlled holography with discretized phase levels. Here we report on spin and wavelength multiplexed nonlinear metasurface holography, which allows construction of multiple target holographic images carried independently by the fundamental and harmonic generation waves of different spins. The nonlinear holograms provide independent, nondispersive and crosstalk-free post-selective channels for holographic multiplexing and multidimensional optical data storages, anti-counterfeiting, and optical encryption. PMID:27306147

  7. Advanced technology development multi-color holography

    NASA Technical Reports Server (NTRS)

    Vikram, Chandra S.

    1994-01-01

    Several key aspects of multi-color holography and some non-conventional ways to study the holographic reconstructions are considered. The error analysis of three-color holography is considered in detail with particular example of a typical triglycine sulfate crystal growth situation. For the numerical analysis of the fringe patterns, a new algorithm is introduced with experimental verification using sugar-water solution. The role of the phase difference among component holograms is also critically considered with examples of several two- and three-color situations. The status of experimentation on two-color holography and fabrication of a small breadboard system is also reported. Finally, some successful demonstrations of unconventional ways to study holographic reconstructions are described. These methods are deflectometry and confocal optical processing using some Spacelab III holograms.

  8. Super-resolution phase reconstruction technique in electron holography with a stage-scanning system

    NASA Astrophysics Data System (ADS)

    Lei, Dan; Mitsuishi, Kazutaka; Harada, Ken; Shimojo, Masayuki; Ju, Dongying; Takeguchi, Masaki

    2014-02-01

    Super-resolution image reconstruction is a digital signal processing technique that allows creating a high-resolution image from multiple low-resolution images taken at slightly different positions. We introduce the super-resolution image reconstruction technique into electron holography for reconstructing phase images as follows: the studied specimen is shifted step-wise with a high-precision piezo holder, and a series of holograms is recorded. When the step size is not a multiple of the CCD pixel size, processing of the acquired series results in a higher pixel density and spatial resolution as compared to the phase image obtained with conventional holography. The final resolution exceeds the limit of the CCD pixel size divided by the magnification.

  9. Spatially resolved, diffuse reflectance imaging for subsurface pattern visualization toward development of a lensless imaging platform: phantom experiments

    NASA Astrophysics Data System (ADS)

    Schelkanova, Irina; Pandya, Aditya; Saiko, Guennadi; Nacy, Lidia; Babar, Hannan; Shah, Duoaud; Lilge, Lothar; Douplik, Alexandre

    2016-01-01

    A portable, spatially resolved, diffuse reflectance lensless imaging technique based on the charge-coupled device or complementary metal-oxide semiconductor sensor directly coupled to the fiber optic bundle is proposed for visualization of subsurface structures such as superficial microvasculature in the epithelium. We discuss an experimental method for emulating a lensless imaging setup via raster scanning a single fiber-optic cable over a microfluidic phantom containing periodic hemoglobin absorption contrast. To evaluate the ability of the technique to recover information about the subsurface linear structures, scattering layers formed of the Sylgard® 184 Silicone Elastomer and titanium dioxide were placed atop the microfluidic phantom. Thickness of the layers ranged from 0.2 to 0.7 mm, and the values of the reduced scattering coefficient (μs‧) were between 0.85 and 4.25 mm-1. The results demonstrate that fiber-optic, lensless platform can be used for two-dimensional imaging of absorbing inclusions in diffuse reflectance mode. In these experiments, it was shown that diffuse reflectance imaging can provide sufficient spatial sampling of the phantom for differentiation of 30 μm structural features of the embedded absorbing pattern inside the scattering media.

  10. DSS-24 microwave holography measurements

    NASA Technical Reports Server (NTRS)

    Rochblatt, D. J.; Withington, P. M.; Jackson, H. J.

    1995-01-01

    The JPL DSN Microwave Antenna Holography System (MAHST) was applied to the newly constructed DSS-24 34-m beam-waveguide antenna at Goldstone, California. The application of MAHST measurements and corrections at DSS 24 provided the critical RF performance necessary to not only meet the project requirements and goals, but to surpass them. A performance increase of 0.35 dB at X-band (8.45 GHz) and 4.9 dB at Ka-band (32 GHz) was provided by MAHST, resulting in peak efficiencies of 75.25 percent at X-band and 60.6 percent at Ka-band (measured from the Cassegrain focus at f1). The MAHST enabled setting the main reflector panels of DSS 24 to 0.25-mm rms, making DSS 24 the highest precision antenna in the NASA/JPL DSN. The precision of the DSS-24 antenna (diameter/rms) is 1.36 x 10(exp 5), and its gain limit is at 95 GHz.

  11. Systems analysis for DSN microwave antenna holography

    NASA Technical Reports Server (NTRS)

    Rochblatt, D. J.

    1989-01-01

    Proposed systems for Deep Space Network (DSN) microwave antenna holography are analyzed. Microwave holography, as applied to antennas, is a technique which utilizes the Fourier Transform relation between the complex far-field radiation pattern of an antenna and the complex aperture field distribution to provide a methodology for the analysis and evaluation of antenna performance. Resulting aperture phase and amplitude distribution data are used to precisely characterize various crucial performance parameters, including panel alignment, subreflector position, antenna aperture illumination, directivity at various frequencies, and gravity deformation. Microwave holographic analysis provides diagnostic capacity as well as being a powerful tool for evaluating antenna design specifications and their corresponding theoretical models.

  12. Holography - Application To Art: Curatorial Observations

    NASA Astrophysics Data System (ADS)

    Dinsmore, Sydney

    1987-06-01

    An exploration of the need to define a specific and critical language to describe the art of holography. Within any discussion of art, critical analysis must maintain an objective openess, particularily when the discourse concerns new media. To apply technological invention to art, new media is often without precedent on which to base criticism and bias. For this reason, holography falls prey to comparative rhetoric and established evaluation of other forms of imaging,as photography emulated the compositional romanticism of painting initially. Isolated and often misunderstood within the context of history, new media vascillates between legitimacy and curiosity in an attempt to create specific parameters to identify perceptual transition.

  13. Anisotropy in Gravity and Holography

    NASA Astrophysics Data System (ADS)

    Melby-Thompson, Charles Milton

    In this thesis, we examine the dynamical structure of Hořava-Lifshitz gravity, and investigate its relationship with holography for anisotropic systems. Hořava-Lifshitz gravity refers to a broad class of gravitational models that incorporate anisotropy at a fundamental level. The idea behind Hořava-Lifshitz gravity is to utilize ideas from the theory of dynamical critical phenomena into gravity to produce a theory of dynamical spacetime that is power-counting renormalizable, and is thus a candidate renormalizable quantum field theory of gravity. One of the most distinctive features of Hořava-Lifshitz gravity is that its group of symmetries consists not of the diffeomorphisms of spacetime, but instead of the group of diffeomorphisms that preserve a given foliation by spatial slices. As a result of having a smaller group of symmetries, HL gravity naturally has one more propagating degree of freedom than general relativity. The extra mode presents two possible difficulties with the theory, one relating to consistency, and the second to its viability as a phenomenological model. (1) It may destabilize the theory. (2) Phenomenologically, there are severe constraints on the existence of an extra propagating graviton polarization, as well as strong experimental constraints on the value of a parameter appearing in the dispersion relation of the extra mode. In the first part of this dissertation we show that the extra mode can be eliminated by introducing a new local symmetry which steps in and takes the place of general covariance in the anisotropic context. While the identification of the appropriate symmetry is quite subtle in the full non-linear theory, once the dust settles, the resulting theory has a spectrum which matches that of general relativity in the infrared. This goes a good way toward answering the question of how close Hořava-Lifshitz gravity can come to reproducing general relativity in the infrared regime. In the second part of the thesis we pursue

  14. Synthetic optical holography for rapid nanoimaging

    PubMed Central

    Schnell, M.; Carney, P. S.; Hillenbrand, R.

    2014-01-01

    Holography has paved the way for phase imaging in a variety of wide-field techniques, including electron, X-ray and optical microscopy. In scanning optical microscopy, however, the serial fashion of image acquisition seems to challenge a direct implementation of traditional holography. Here we introduce synthetic optical holography (SOH) for quantitative phase-resolved imaging in scanning optical microscopy. It uniquely combines fast phase imaging, technical simplicity and simultaneous operation at visible and infrared frequencies with a single reference arm. We demonstrate SOH with a scattering-type scanning near-field optical microscope (s-SNOM) where it enables reliable quantitative phase-resolved near-field imaging with unprecedented speed. We apply these capabilities to nanoscale, non-invasive and rapid screening of grain boundaries in CVD-grown graphene, by recording 65 kilopixel near-field images in 26 s and 2.3 megapixel images in 13 min. Beyond s-SNOM, the SOH concept could boost the implementation of holography in other scanning imaging applications such as confocal microscopy. PMID:24651276

  15. Advanced technology development multi-color holography

    NASA Technical Reports Server (NTRS)

    Vikram, Chandra S.

    1993-01-01

    This is the final report of the Multi-color Holography project. The comprehensive study considers some strategic aspects of multi-color holography. First, various methods of available techniques for accurate fringe counting are reviewed. These are heterodyne interferometry, quasi-heterodyne interferometry, and phase-shifting interferometry. Phase-shifting interferometry was found to be the most suitable for multi-color holography. Details of experimentation with a sugar solution are also reported where better than 1/200 of a fringe order measurement capability was established. Rotating plate glass phase shifter was used for the experimentation. The report then describes the possible role of using more than two wavelengths with special reference-to-object beam intensity ratio needs in multicolor holography. Some specific two- and three-color cases are also described in detail. Then some new analysis methods of the reconstructed wavefront are considered. These are deflectometry, speckle metrology, confocal optical signal processing, and phase shifting technique related applications. Finally, design aspects of an experimental breadboard are presented.

  16. Entangled States, Holography and Quantum Surfaces

    SciTech Connect

    Chapline, G F

    2003-08-13

    Starting with an elementary discussion of quantum holography, we show that entangled quantum states of qubits provide a ''local'' representation of the global geometry and topology of quantum Riemann surfaces. This representation may play an important role in both mathematics and physics. Indeed, the simplest way to represent the fundamental objects in a ''theory of everything'' may be as muti-qubit entangled states.

  17. Generalized in-line digital holographic technique based on intensity measurements at two different planes

    NASA Astrophysics Data System (ADS)

    Situ, Guohai; Ryle, James P.; Gopinathan, Unnikrishnan; Sheridan, John T.

    2008-02-01

    In-line digital holography based on two-intensity measurements [Zhang et al. Opt. Lett. 29, 1787 (2004)], is modified by introducing a π shifting in the reference phase. Such an improvement avoids the assumption that the object beam must be much weaker than the reference beam in strength and results in a simplified experimental implementation. Computer simulations and optical experiments are carried out to validate the method, which we refer to as position-phase-shifting digital holography.

  18. Digital holographic imaging for three-dimensional particle and flow measurements

    NASA Astrophysics Data System (ADS)

    Pan, Gang

    Digital holographic imaging is a strong contender as the next-generation technique for dynamic three-dimensional (3D) measurement of particles and fluids flows. With the digital holographic approach, holograms of particles or flow tracers are directly recorded on a digital camera and then numerically reconstructed. The 3D information of particles and local flow fields is extracted from reconstructed image field using information processing algorithms specifically designed for digital particle holography. This dissertation describes a framework of digital particle holography intended for practical use in 3D particle and fluid flow measurements. This framework consists of three components, namely digital recording, numerical reconstruction, and information processing. Based on experiments and computer simulations, the following questions are answered in this dissertation; (1) Digital recording: Which holography schemes should be used and what is the resolution requirement in digital particle holography? (2) Numerical reconstruction: How to numerically reconstruct particle holograms and what are the practical considerations? (3) Information processing: What are the challenges in digital particle holography and what are the best solutions? Based on the framework outlined in this dissertation, two holographic imaging systems have been designed, implemented and tested. The first system is based on in-line holography and uses very simple optics. It features a complex-amplitude based method for particle extraction which significantly improves the depth resolution of digital particle holography. The system has been tested in 1D channel flow experiment and the results show that statistically the error of velocity measurement is only 1.7% based on mean flow velocity and 1.5% based on continuity equation. The in-line system has also been used to measure flow past a cylinder and produces time-series volumetric and 3D measurement of the velocity field at Re = 2,200. The second

  19. Single-pixel phase-corrected fiber bundle endomicroscopy with lensless focussing capability

    PubMed Central

    Gordon, George S.D.; Joseph, James; Bohndiek, Sarah E.; Wilkinson, Timothy D.

    2016-01-01

    In this paper a novel single-pixel method for coherent imaging through an endoscopic fiber bundle is presented. The use of a single-pixel detector allows greater sensitivity over a wider range of wavelengths, which could have significant applications in endoscopic fluorescence microscopy. First, the principle of lensless focussing at the distal end of a coherent fiber bundle is simulated to examine the impact of pixelation at microscopic scales. Next, an experimental optical correlator system using spatial light modulators (SLMs) is presented. A simple contrast imaging method of characterizing and compensating phase aberrations introduced by fiber bundles is described. Experimental results are then presented showing that our phase compensation method enables characterization of the optical phase profile of individual fiberlets. After applying this correction, early results demonstrating the ability of the system to electronically adjust the focal plane at the distal end of the fiber bundle are presented. The structural similarity index (SSIM) between the simulated image and the experimental focus-adjusted image increases noticeably when the phase correction is applied and the retrieved image is visually recognizable. Strategies to improve image quality are discussed. PMID:27279676

  20. HyperCube: A Small Lensless Position Sensing Device for the Tracking of Flickering Infrared LEDs.

    PubMed

    Raharijaona, Thibaut; Mignon, Paul; Juston, Raphaël; Kerhuel, Lubin; Viollet, Stéphane

    2015-07-08

    An innovative insect-based visual sensor is designed to perform active marker tracking. Without any optics and a field-of-view of about 60°, a novel miniature visual sensor is able to locate flickering markers (LEDs) with an accuracy much greater than the one dictated by the pixel pitch. With a size of only 1 cm3 and a mass of only 0.33 g, the lensless sensor, called HyperCube, is dedicated to 3D motion tracking and fits perfectly with the drastic constraints imposed by micro-aerial vehicles. Only three photosensors are placed on each side of the cubic configuration of the sensing device, making this sensor very inexpensive and light. HyperCube provides the azimuth and elevation of infrared LEDs flickering at a high frequency (>1 kHz) with a precision of 0.5°. The minimalistic design in terms of small size, low mass and low power consumption of this visual sensor makes it suitable for many applications in the field of the cooperative flight of unmanned aerial vehicles and, more generally, robotic applications requiring active beacons. Experimental results show that HyperCube provides useful angular measurements that can be used to estimate the relative position between the sensor and the flickering infrared markers.

  1. On-chip integrated lensless fluorescence microscopy/spectroscopy module for cell-based sensors

    NASA Astrophysics Data System (ADS)

    Li, Wei; Knoll, Thorsten; Sossalla, Adam; Bueth, Heiko; Thielecke, Hagen

    2011-03-01

    The integration of a fluorescence microscopy/spectroscopy module in cell-based lab-on-a-chip systems is of high interest for applications in cell-based diagnostics and substance evaluation in situ. We present an on-chip integrated lensless fluorescence imaging module applying the principle of contact/proximate optical lithography. The pixel resolution is comparable with a 4 x objective microscope. The module can be used for morphology and fluorescence imaging of mammalian cells (15 - 20 μm) as well as for testing the concentration of a fluorescent substance. The biological samples or solutions are sustained in disposable sterilized microfluidic chips with 1 μm thick silicon nitride (Si3N4) membranes. These chips are assembled on the surface of a 5 megapixel colored CMOS image sensor array with 1.75 μm pixel size, which is coated with an additional interference filter. Each culturing chip consists of a MEMS cavity chip and a PDMS microfluidic interface. The surface of the CMOS image sensor is smoothened using SU-8 photoresist spin-coating for a commercial grade interference filter (optical density >= 5) coating by Plasma-Ion Assisted Deposition thereafter. The function is demonstrated by primary imaging results of the non-/fluorescent mammalian cells/microspheres as well as by differentiating different concentrations of FITC solutions.

  2. On-chip integrated lensless microscopy module for optical monitoring of adherent growing mammalian cells.

    PubMed

    Li, Wei; Knoll, Thorsten; Thielecke, Hagen

    2010-01-01

    Lab-on-a-chip systems are increasingly applied in cell-based assays for toxicology and drug testing. In this paper, an on-chip integrated lensless microscopy module using a direct projection method for optical monitoring of the shadow images of adherent growing mammalian cells is presented. The biological cells are conserved and interfaced by a microfabricated cavity chip with a 1 microm thick silicon nitride (Si(3)N(4)) substrate onto the surface of a 5 megapixel CMOS image sensor with 2.2 microm pixel size. The optical resolution of the assembly is estimated by the contact/proximate printing theory from optical lithography. Further characterization is made by imaging microbeads in chips with the Si(3)N(4)-membrane as well as in cavity chips with membranes made from dry film resist (DFR, thickness 20, 40 and 60 microm). The module represents a 3 × optical microscope for cell morphology imaging. The function is demonstrated by the growth monitoring of L929 cells cultured in cavity chips with Si(3)N(4) substrate for 2 days and by checking the colorimetric staining of cells with a compromised membrane. PMID:21096993

  3. HyperCube: A Small Lensless Position Sensing Device for the Tracking of Flickering Infrared LEDs

    PubMed Central

    Raharijaona, Thibaut; Mignon, Paul; Juston, Raphaël; Kerhuel, Lubin; Viollet, Stéphane

    2015-01-01

    An innovative insect-based visual sensor is designed to perform active marker tracking. Without any optics and a field-of-view of about 60°, a novel miniature visual sensor is able to locate flickering markers (LEDs) with an accuracy much greater than the one dictated by the pixel pitch. With a size of only 1 cm3 and a mass of only 0.33 g, the lensless sensor, called HyperCube, is dedicated to 3D motion tracking and fits perfectly with the drastic constraints imposed by micro-aerial vehicles. Only three photosensors are placed on each side of the cubic configuration of the sensing device, making this sensor very inexpensive and light. HyperCube provides the azimuth and elevation of infrared LEDs flickering at a high frequency (>1 kHz) with a precision of 0.5°. The minimalistic design in terms of small size, low mass and low power consumption of this visual sensor makes it suitable for many applications in the field of the cooperative flight of unmanned aerial vehicles and, more generally, robotic applications requiring active beacons. Experimental results show that HyperCube provides useful angular measurements that can be used to estimate the relative position between the sensor and the flickering infrared markers. PMID:26184193

  4. Image analysis of modified Hamming aperture: application on confocal microscopy and holography

    NASA Astrophysics Data System (ADS)

    Hamed, A. M.; Alsaeed, T. A.

    2015-06-01

    Digital truncated and obstructed Hamming apertures are used in confocal laser scanning microscope (CLSM) to improve the resolution. The resultant point spread function (RPSF) and the coherent transfer function of the defined microscope, using the modified Hamming apertures, are computed. The mammographic images are used in the CLSM to test resolution of the investigated apertures. The image is computed from the modulus square of the convolution product of the RPSF and the complex amplitude of the object. A comparison with the images obtained in case of circular uniform apertures is investigated. Another application on holography allows the recognition of the modulated Hamming apertures.

  5. Industrial Holography Combined With Image Processing

    NASA Astrophysics Data System (ADS)

    Schorner, J.; Rottenkolber, H.; Roid, W.; Hinsch, K.

    1988-01-01

    Holographic test methods have gained to become a valuable tool for the engineer in research and development. But also in the field of non-destructive quality control holographic test equipment is now accepted for tests within the production line. The producer of aircraft tyres e. g. are using holographic tests to prove the guarantee of their tyres. Together with image processing the whole test cycle is automatisized. The defects within the tyre are found automatically and are listed on an outprint. The power engine industry is using holographic vibration tests for the optimization of their constructions. In the plastics industry tanks, wheels, seats and fans are tested holographically to find the optimum of shape. The automotive industry makes holography a tool for noise reduction. Instant holography and image processing techniques for quantitative analysis have led to an economic application of holographic test methods. New developments of holographic units in combination with image processing are presented.

  6. Imaging nanoparticles in cells by nanomechanical holography

    NASA Astrophysics Data System (ADS)

    Tetard, Laurene; Passian, Ali; Venmar, Katherine T.; Lynch, Rachel M.; Voy, Brynn H.; Shekhawat, Gajendra; Dravid, Vinayak P.; Thundat, Thomas

    2008-08-01

    Nanomaterials have potential medical applications, for example in the area of drug delivery, and their possible adverse effects and cytotoxicity are curently receiving attention. Inhalation of nanoparticles is of great concern, because nanoparticles can be easily aerosolized. Imaging techniques that can visualize local populations of nanoparticles at nanometre resolution within the structures of cells are therefore important. Here we show that cells obtained from mice exposed to single-walled carbon nanohorns can be probed using a scanning probe microscopy technique called scanning near field ultrasonic holography. The nanohorns were observed inside the cells, and this was further confirmed using micro Raman spectroscopy. Scanning near field ultrasonic holography is a useful technique for probing the interactions of engineered nanomaterials in biological systems, which will greatly benefit areas in drug delivery and nanotoxicology.

  7. Chaos in AdS_{2} Holography.

    PubMed

    Jensen, Kristan

    2016-09-01

    We revisit two-dimensional holography with the Sachdev-Ye-Kitaev models in mind. Our main result is to rewrite a generic theory of gravity near a two-dimensional anti-de Sitter spacetime throat as a novel hydrodynamics coupled to the correlation functions of a conformal quantum mechanics. This gives a prescription for the computation of n-point functions in the dual quantum mechanics. We thereby find that the dual is maximally chaotic. PMID:27661678

  8. Chaos in AdS2 Holography

    NASA Astrophysics Data System (ADS)

    Jensen, Kristan

    2016-09-01

    We revisit two-dimensional holography with the Sachdev-Ye-Kitaev models in mind. Our main result is to rewrite a generic theory of gravity near a two-dimensional anti-de Sitter spacetime throat as a novel hydrodynamics coupled to the correlation functions of a conformal quantum mechanics. This gives a prescription for the computation of n -point functions in the dual quantum mechanics. We thereby find that the dual is maximally chaotic.

  9. Electron holography of devices with epitaxial layers

    SciTech Connect

    Gribelyuk, M. A. Ontalus, V.; Baumann, F. H.; Zhu, Z.; Holt, J. R.

    2014-11-07

    Applicability of electron holography to deep submicron Si devices with epitaxial layers is limited due to lack of the mean inner potential data and effects of the sample tilt. The mean inner potential V{sub 0} = 12.75 V of the intrinsic epitaxial SiGe was measured by electron holography in devices with Ge content C{sub Ge} = 18%. Nanobeam electron diffraction analysis performed on the same device structure showed that SiGe is strain-free in [220] direction. Our results showed good correlation with simulations of the mean inner potential of the strain-free SiGe using density function theory. A new method is proposed in this paper to correct electron holography data for the overlap of potentials of Si and the epitaxial layer, which is caused by the sample tilt. The method was applied to the analysis of the dopant diffusion in p-Field-effect Transistor devices with the identical gate length L = 30 nm, which had alternative SiGe geometry in the source and drain regions and was subjected to different thermal processing. Results have helped to understand electrical data acquired from the same devices in terms of dopant diffusion.

  10. Guest Editoria Holography A Dedication To Dennis Gabor

    NASA Astrophysics Data System (ADS)

    Wyant, James C.; Chen, Chungte T.; Reynolds, George O.

    1980-10-01

    We thought it was time to bring the readers of Optical Engineering up to date on the new developments in holography, as was done exactly five years ago with the September/October 1975 special issue. The seeds for this special issue were sown during the 1978 Gordon Conference on Coherent Optics and Holography held at the Miramar Hotel in Santa Barbara, California. The conference clearly demonstrated, through the discussion of many interesting research and development applications, that holography is alive and well. For example, artists have discovered holography as a new medium and there is a museum for displaying new holographic developments and inventions. In addition to the opening of the New York museum where artists and patrons are thoroughly enjoying holography, the past five years have witnessed the publication of the Handbook of Optical Holography, continuing issues of the Acoustical Holography proceedings, the gathering of scientists to exchange ideas at three Gordon Conferences in 1976, 1978, and 1980, and the SPIE conference on holography held in Los Angeles in February 1980.

  11. Laser Holography. High Tech with High Potential for Learning Activities.

    ERIC Educational Resources Information Center

    Schlegel, Ronald D.

    1986-01-01

    This article discusses the procedure, historical development, and applications of holography, and discusses the feasibility and value of implementing a unit or course of study of laser holography into an existing photography laboratory and curriculum. An equipment and supplies list, giving supplies and cost, is included. (CT)

  12. Lensless imaging for simultaneous microfluidic sperm monitoring and sorting†‡

    PubMed Central

    Zhang, Xiaohui; Khimji, Imran; Gurkan, Umut Atakan; Safaee, Hooman; Catalano, Paolo Nicolas; Keles, Hasan Onur; Kayaalp, Emre

    2013-01-01

    5.3 million American couples of reproductive age (9%) are affected by infertility, among which male factors account for up to 50% of cases, which necessitates the identification of parameters defining sperm quality, including sperm count and motility. In vitro fertilization (IVF) with or without intra cytoplasmic sperm injection (ICSI) has become the most widely used assisted reproductive technology (ART) in modern clinical practice to overcome male infertility challenges. One of the obstacles of IVF and ICSI lies in identifying and isolating the most motile and presumably healthiest sperm from semen samples that have low sperm counts (oligozoospermia) and/or low sperm motility (oligospermaesthenia). Microfluidic systems have shown potential to sort sperm with flow systems. However, the small field of view (FOV) of conventional microscopes commonly used to image sperm motion presents challenges in tracking a large number of sperm cells simultaneously. To address this challenge, we have integrated a lensless charge-coupled device (CCD) with a microfluidic chip to enable wide FOV and automatic recording as the sperm move inside a microfluidic channel. The integrated system enables the sorting and tracking of a population of sperm that have been placed in a microfluidic channel. This channel can be monitored in both horizontal and vertical configuration similar to a swim-up column method used clinically. Sperm motilities can be quantified by tracing the shadow paths for individual sperm. Moreover, as the sperm are sorted by swimming from the inlet towards the outlet of a microfluidic channel, motile sperm that reach the outlet can be extracted from the channel at the end of the process. This technology can lead to methods to evaluate each sperm individually in terms of motility response in a wide field of view, which could prove especially useful, when working with oligozoospermic or oligospermaesthenic samples, in which the most motile sperm need to be isolated from

  13. Three-dimensional imaging characteristics and depth resolution in digital holographic three-dimensional imaging spectrometry

    NASA Astrophysics Data System (ADS)

    Obara, Masaki; Yoshimori, Kyu

    2015-07-01

    A four-dimensional impulse response function for the digital holographic three-dimensional imaging spectrometry has been fully derived in closed form. Due to its factorizing nature of the mathematical expression of four-dimensional impulse response function, three-dimensional spatial part of impulse response function directly corresponds to threedimensional point spread function of in-line digital holography with rectangular aperture. Based on these mathematical results, this paper focuses on the investigation of spectral resolution and three-dimensional spatial resolution in digital holographic three-dimensional imaging spectrometry and digital holography. We found that the theoretical prediction agree well with the experimental results. This work suggests a new criterion and estimate method regarding threedimensional spatial resolution of in-line digital holography.

  14. Fresnel incoherent correlation holography (FINCH): a review of research

    NASA Astrophysics Data System (ADS)

    Rosen, Joseph; Brooker, Gary

    2012-07-01

    In this review, we describe our method for creating holograms of incoherent objects, dubbed Fresnel incoherent correlation holography (FINCH). FINCH creates holograms by a single-channel on-axis incoherent interferometer process. Like any Fresnel hologram, the object is correlated with quadratic phase functions, but the correlation is carried out without any movement. Generally, in the FINCH system, light is reflected, or emitted, from a three-dimensional (3D) object, propagates through a spatial light modulator (SLM), and is recorded by a digital camera. The SLM is used as a beam-splitter of the single-channel incoherent interferometer, such that each spherical beam originated from each object point is split into two spherical beams with two different curve radiuses. Incoherent summing of the entire interferences between all the couples of the spherical beams creates the Fresnel hologram of the observed 3D object. When this hologram is reconstructed in the computer, the 3D properties of the object are revealed. In this review, we describe various aspects of FINCH which have been described recently, including FINCH of reflected white light, FINCH of fluorescence objects, a FINCH-based holographic fluorescence microscope, a FINCH configuration which capitalizes on the polarization sensitivity of the SLM and finally FINCH is analyzed in view of linear system theory.

  15. Real-Time Moire Holography

    NASA Astrophysics Data System (ADS)

    Soares, O. D. D.; Lage, A. I. V. S.

    1986-08-01

    Interferometric techniques including hologrametry, both classical and electronic, present high sensitivity making difficult its practical use in real-time. The introduction of the differencial concept as moire evaluation techniques permits to use with advantage an arbitrary reference pattern within the correlation range. The carrier spatial spectrum can be directly the interferogram fringe pattern instead of the original interference pattern of wavelength dimensional scale. A moire techniques is in itself an optical processing method reducing evaluation time which is advantageous when real-time response is desired from hybrid metrological systems. The moire evaluation is performed via a dynamical digital memory that executes arithmetic operations on two frames temporally in sequence, at TV rate. These characteristics of the moire evaluation techniques can be implemented on a real-time holographic (or speckle based) hybrid system with great practical advantage for dynamical studies.

  16. Ethereal presences in holography and photography

    NASA Astrophysics Data System (ADS)

    Richardson, M.; Byrne, Kay

    2007-02-01

    This paper examines the concept of the 'Presence of Absence' in post-mortem photography and holography, drawing upon both historical and lesser-known images as reference. To create a photographic negative one needs the presence of light to expose the light sensitive surface, be it glass, a polished plate or plastic. A hologram may also be created when a coherent light source, for example from a Laser, travels through a light sensitive material and falls upon the subject to be recorded. A holograph however, retains the optical qualities of both phase and amplitude, the memory of light. Both mediums recall, as it were, 'now absent moments', and confronts us with what is 'not there' as much as 'what is'. This paper examines the exploration of absence and presence in post-mortem photography and holography and it's a richly visceral visual language. A photonic syntax can interpret death as an elegant yet horrific aesthetic, the photograph may be beautify screened and yet obscene in its content. In essence one can be a voyeur, experiencing a mere visual whisper of the true nature of the subject. Our Victorian forefathers explored postmortem photography as an object of mourning, and at the close of the nineteenth century when Jack the Ripper had the inhabitants of White Chapel in a grip of fear, photography made its mark as a documentation of violent crime. Today, within contemporary photography, death is now presented within the confines of the 'Art Gallery', as a sensual, and at times, sensationalised art form. In exploring post-mortem imagery, both in holography and conventional photography, absence presents an aspect of death as startling in its unanimated form and detailed in its finite examination of mortality.

  17. Investigation of particles located in the water by digital holography

    NASA Astrophysics Data System (ADS)

    Dyomin, V. V.; Kamenev, D. V.

    2016-03-01

    An automatic technique for processing holograms of particles present in water is proposed. Its applications to determine particle concentration and their size distribution are illustrated with examples. The method is validated using waterborne air bubbles and particles settling in water. The use of video based on holographic data is considered for dynamic investigation of these particles. This video consists of sequences of holographic images of particles and the concentration and particle size distribution is studied in dynamics.

  18. Dual-wavelength digital holography: single shot calibration

    NASA Astrophysics Data System (ADS)

    Khodadad, Davood; Bergström, Per; Hällstig, Emil; Sjödahl, Mikael

    2014-07-01

    In an on line shape measurement in disturbed environment, use of many wavelengths in order to avoid phase ambiguity may become a problem as it is necessary to acquire all holograms simultaneously due to environmental disturbances. Therefore to make the shape data available the different holograms have to be extracted from a single recorded image in spectral domain. Appropriate cut areas in the Fourier method are therefore of great importance for decoding information carried by different wavelengths. Furthermore using different laser sources, induces aberration and pseudo phase changes which must be compensated. To insure any phase change is only because of the object shape, calibration is therefore indispensable. For this purpose, effects of uncontrolled carrier frequency filtering are discussed. A registration procedure is applied using minimum speckle displacements to find the best cut area to extract and match the interference terms. Both holograms are numerically propagated to a focus plane to avoid any unknown errors. Deviations between a reference known plate and its measurement are found and used for calibration. We demonstrate that phase maps and speckle displacements can be recovered free of chromatic aberrations. To our knowledge, this is the first time that a single shot dual wavelength calibration is reported by defining a criteria to make the spatial filtering automatic avoiding the problems of manual methods. The procedure is shown to give shape accuracy of 35μm with negligible systematic errors using a synthetic wavelength of 1.1 mm.

  19. Observation of surfaces by reflection electron holography.

    PubMed

    Osakabe, N

    1992-02-15

    Reflection electron holography is described as a method to observe sub-A surface morphology. Phase shift of a Bragg-reflected electron wave was measured by means of holographic interferometry using an electron microscope equipped with a field emission electron gun and an electron biprism. A short wavelength of high energy electrons is the essential key to the high vertical sensitivity of this method, since geometrical path differences produced by the surface topography are measured in units of wavelengths in interferometrical measuring. Phase shift at a monoatomic step and the displacement field around a dislocation emerging on the surface were observed.

  20. Art of color holography: pioneers in change

    NASA Astrophysics Data System (ADS)

    Richardson, Martin J.; Bjelkhagen, Hans I.

    2000-10-01

    The possibility to easily record full color holograms, (simply color holograms) has opened new possibilities for art holographers. This paper includes details concerning preparation of subject matter and its practical suitability for color holographic recordings from practical working sessions at ARTCAPI Atelier de Recherche Technique et de Creation Artistique en Physique et en Informatique in France. Martin Richardson as invited artist and Hans Bjelkhagen as scientist holographer describe color holography to a wider public audience through artistic display. Both directly recorded true color images and computer-generated images based on the ZEBRA printing technique are to be presented.

  1. "Kunstwerk" in the age of holography

    NASA Astrophysics Data System (ADS)

    Öhlmann, Dietmar; Meulien Ohlmann, Odile

    2013-02-01

    In 2007 one auction shocked collectors and artists: Two art works of Rudie Berkhout were offered for one thousand dollars on eBay. The closing down of museums for holography in Cologne, New York and Washington DC paid a big price of respect for true art. Benjamin's vision about "Art in the Age of Mechanical Reproduction" found a new expression in abuse and ignorance. Beautiful sculptures of Art are reduced to "Holograms" like Rudie's "Cairo Aspect" placed in a rummage sale, covered by fingerprints of ignorance.

  2. Multi-field inflation from holography

    SciTech Connect

    Garriga, Jaume; Urakawa, Yuko; Skenderis, Kostas E-mail: K.Skenderis@soton.ac.uk

    2015-01-01

    We initiate the study of multi-field inflation using holography. Bulk light scalar fields correspond to nearly marginal operators in the boundary theory and the dual quantum field theory is a deformation of a CFT by such operators. We compute the power spectra of adiabatic and entropy perturbations in a simple model and find that the adiabatic curvature perturbation is not conserved in the presence of entropy perturbations but becomes conserved when the entropy perturbations are set to zero or the model is effectively a single scalar model, in agreement with expectations from cosmological perturbation theory.

  3. Electron holography for fields in solids: problems and progress.

    PubMed

    Lichte, Hannes; Börrnert, Felix; Lenk, Andreas; Lubk, Axel; Röder, Falk; Sickmann, Jan; Sturm, Sebastian; Vogel, Karin; Wolf, Daniel

    2013-11-01

    Electron holography initially was invented by Dennis Gabor for solving the problems raised by the aberrations of electron lenses in Transmission Electron Microscopy. Nowadays, after hardware correction of aberrations allows true atomic resolution of the structure, for comprehensive understanding of solids, determination of electric and magnetic nanofields is the most challenging task. Since fields are phase objects in the TEM, electron holography is the unrivaled method of choice. After more than 40 years of experimental realization and steady improvement, holography is increasingly contributing to these highly sophisticated and essential questions in materials science, as well to the understanding of electron waves and their interaction with matter. PMID:23831133

  4. Modeling and design of micromachined optical Söller collimators for lensless CCD-based fluorometry.

    PubMed

    Balsam, Joshua; Ossandon, Miguel; Bruck, Hugh Alan; Rasooly, Avraham

    2012-11-01

    To address the needs of medical diagnostics in resource-poor settings, it is necessary to develop low cost, simple and portable Point of Care detectors for integrated medical diagnostics. Previously, we have described a simple lensless fluorometer with sensitivity in the range of current ELISA plate readers. The key to the lensfree fluorometer is the uniform spatial distribution of light, which we achieved using a simple optical collimator based on a "stack of pinholes" (a stack of black PMMA plates with arrays of pinholes machined via laser) enabling the light to be collimated from the LED light source through the necessary wavelength filters and the assay's microfluidics directly onto the CCD without a lens. In this paper, we describe the optical principle for designing these Söller collimators for lensfree CCD-based fluorometry. The illuminating surface was modeled as a collection of differential areas emitting uniformly and spherically, and the intensity contribution of each emitting area was summed over the detector surface. To compute the final light intensity distribution from such a differential model we derived an integral equation to sum the individual intensity contributions from the two-dimensional emitting surface. The equation is for a single-hole collimator. Light intensity measurements were taken by placing a collimator with a particular aspect ratio (the ratio of hole length to diameter (L/d)) over the CCD image sensor and capturing an image. The resulting image is the 2D light intensity profile generated by the collimator. As the aspect ratio is increased the slope of the light intensity profile increases, corresponding to an increased degree of collimation. To test the model, the measured maximum and mean light intensities were compared with the theoretical predictions generated from the model. There was an agreement between the variation of the mean (R(2) = 0.990) and maximum (R(2) = 0.938) values of light intensities with aspect ratios based

  5. Introductory overview of holography and speckle

    NASA Astrophysics Data System (ADS)

    Parker, David E.

    1990-10-01

    The purpose of this paper is to provide a nonmathematical overview of holographic and speckle interferometry. This provides information necessary for those participants with little or no background in these areas to understand the application papers in the Holography Speckle session of ICALEO''89. Holography A hologram is an optical device which is capable of producing threedimensional images. The word hologram stems from the Greek root holos which means whole complete or entire and the word gram which means message. Thus a hologram is a complete record of a scene or object. The first hologram was produced by Dennis Gabor in 1948. In 1971 he won the Nobel Prize for his work. Leith and Upatnieks produced the first holograms using a laser in the early 1960''s. In conventional photography the light reflected from a scene is focused by a camera lens onto a photographic emulsion. The variation in irradiance of the light reaching the emulsion is related only to the amplitude of the electric field due to the light. To produce three-dimensional images a hologram records information about both the amplitude and phase of light reflected from a scene or object. Production Hologram Figure 1 is a sketch of a system which can be used to produce optical holograms. A laser operating in the TEM mode is used as a light source. A continuous wave or a pulsed laser can be

  6. High-resolution imaging using endoscopic holography

    NASA Astrophysics Data System (ADS)

    Bjelkhagen, Hans I.

    1990-08-01

    Endoscopic holography or endoholography combines the features of endoscopy and holography. The purpose of endoholographic imaging is to provide the physician with a unique means of extending diagnosis by providing a life-like record of tissue. Endoholographic recording will provide means for microscopic examination of tissue and in some cases may obviate the need to excise specimens for biopsy. In this method holograms which have the unique properties of three-dimensionality large focal depth and high resolution are made with a newly designed endoscope. The endoscope uses a single-mode optical fiber for illumination and single-beam reflection holograms are recorded in close contact with the tissue at the distal end of the endoscope. The holograms are viewed under a microscope. By using the proper combinations of dyes for staining specific tissue types with various wavelengths of laser illumination increased contrast on the cellular level can be obtained. Using dyes such as rose bengal in combination with the 514. 5 nm line of an argon ion laser and trypan blue or methylene blue with the 647. 1 nm line of a krypton ion laser holograms of the stained colon of a dog showed the architecture of the colon''s columnar epithelial cells. It is hoped through chronological study using this method in-vivo an increased understanding of the etiology and pathology of diseases such as Crohn''s diseases colitis proctitis and several different forms of cancer will help to their control. 1.

  7. Massively parallel X-ray holography

    SciTech Connect

    Spence, John C.H; Marchesini, Stefano; Boutet, Sebastien; Sakdinawat, Anne E.; Bogan, Michael J.; Bajt, Sasa; Barty, Anton; Chapman, Henry N.; Frank, Matthias; Hau-Riege, Stefan P.; Szöke, Abraham; Cui, Congwu; Shapiro, David A.; Howells, MAlcolm R.; Shaevitz, Joshua W; Lee, Joanna Y.; Hajdu, Janos; Seibert, Marvin M.

    2008-08-01

    Advances in the development of free-electron lasers offer the realistic prospect of nanoscale imaging on the timescale of atomic motions. We identify X-ray Fourier-transform holography1,2,3 as a promising but, so far, inefficient scheme to do this. We show that a uniformly redundant array4 placed next to the sample, multiplies the efficiency of X-ray Fourier transform holography by more than three orders of magnitude, approaching that of a perfect lens, and provides holographic images with both amplitude- and phase-contrast information. The experiments reported here demonstrate this concept by imaging a nano-fabricated object at a synchrotron source, and a bacterial cell with a soft-X-ray free-electron laser, where illumination by a single 15-fs pulse was successfully used in producing the holographic image. As X-ray lasers move to shorter wavelengths we expect to obtain higher spatial resolution ultrafast movies of transient states of matter

  8. Self-comparative holography and shearography

    NASA Astrophysics Data System (ADS)

    Shang, Huai M.

    2002-05-01

    Double-exposure holography and double-exposure shearography are two interferometric techniques that are particularly useful for precision measurement, and for non-destructive inspection and evaluation of the structural integrity of test objects. Quite often, objects with symmetrical deformation are tested and the challenge to the engineer is to check for symmetry in the deformation. While the holographic and shearographic fringe patterns are able to achieve this, it is felt that the inspection process can be simplified and expedite considerably if the engineer needs only to look for either the absence or the presence of fringes. In other words, a 'go, no-go' gauging method is desired so that the absence of interference fringes depicts symmetrical deformation and the presence of interference fringes depicts asymmetrical deformation. This paper demonstrates that this gauging method can be realized with the use of a commercial beam-splitting cube. By carefully orienting the beam-splitting cube, the image of the test object is brought to overlap, either fully or partially, with the mirror-image of the test object. With fully overlapping images, the relative displacement between two symmetrically located points on the test surface is obtained, resulting in the technique of self-comparative holography. This method is found useful in checking the symmetricity of deformation. With partially overlapping images, the relative displacement-derivative between two symmetrically located points on the test surface is obtained, resulting in the technique of self-comparative shearography.

  9. Simulated electron holography of PSD particles

    NASA Astrophysics Data System (ADS)

    Conbhuí, Pádraig Ó.; Williams, Wyn; Nagy, Les

    2016-04-01

    Electron holography is an experimental technique that is capable of observing magnetic microstructures on the same scale as can be determined using numerical modeling and thus bridge the gap between experimental measurements and theory. I will present a technique for simulating holographic images from the results of micromagnetic models and demonstrate an easily used tool for generating holograms on the fly in an interactive environment (ie in ParaView). Since holography flattens 3D information onto a 2D image, some useful information can be lost. By looking at some examples of holograms of interesting 3D magnetizations (ie PSD structures), particularly how they change as they're rotated, along with comparisons of different structures, I will examine what information can be retrieved and what might be lost. The existance of an external dipole can be indicative of an in-plane component of a seemingly out-of-plane vortex core. It is also seen, however, that two quite different structures (in this case a [111] vortex core and a [111] uniform magnetization) can sometimes be quite indistinguishable.

  10. Absorbing New Subjects: Holography as an Analog of Photography

    NASA Astrophysics Data System (ADS)

    Johnston, Sean F.

    2006-05-01

    I discuss the early history of holography and explore how perceptions, applications, and forecasts of the subject were shaped by prior experience. I focus on the work of Dennis Gabor (1900 1979) in England,Yury N. Denisyuk (b. 1924) in the Soviet Union, and Emmett N. Leith (1927 2005) and Juris Upatnieks (b. 1936) in the United States. I show that the evolution of holography was simultaneously promoted and constrained by its identification as an analog of photography, an association that influenced its assessment by successive audiences of practitioners, entrepreneurs, and consumers. One consequence is that holography can be seen as an example of a modern technical subject that has been shaped by cultural influences more powerfully than generally appreciated. Conversely, the understanding of this new science and technology in terms of an older one helps to explain why the cultural effects of holography have been more muted than anticipated by forecasters between the 1960s and 1990s.

  11. Particle and flow field holography: A critical survey

    NASA Technical Reports Server (NTRS)

    Trolinger, James D.

    1987-01-01

    A brief background is provided for the fields of particle and flow visualization holography. A summary of methods currently in use is given, followed by a discussion of more recent and unique applications. The problem of data reduction is discussed. A state of the art summary is then provided with a prognosis of the future of the field. Particle and flow visualization holography are characterized as powerful tools currently in wide use and with significant untapped potential.

  12. Neural-net Processed Electronic Holography for Rotating Machines

    NASA Technical Reports Server (NTRS)

    Decker, Arthur J.

    2003-01-01

    This report presents the results of an R&D effort to apply neural-net processed electronic holography to NDE of rotors. Electronic holography was used to generate characteristic patterns or mode shapes of vibrating rotors and rotor components. Artificial neural networks were trained to identify damage-induced changes in the characteristic patterns. The development and optimization of a neural-net training method were the most significant contributions of this work, and the training method and its optimization are discussed in detail. A second positive result was the assembly and testing of a fiber-optic holocamera. A major disappointment was the inadequacy of the high-speed-holography hardware selected for this effort, but the use of scaled holograms to match the low effective resolution of an image intensifier was one interesting attempt to compensate. This report also discusses in some detail the physics and environmental requirements for rotor electronic holography. The major conclusions were that neural-net and electronic-holography inspections of stationary components in the laboratory and the field are quite practical and worthy of continuing development, but that electronic holography of moving rotors is still an expensive high-risk endeavor.

  13. Simultaneous Conoscopic Holography and Raman Spectroscopy

    NASA Technical Reports Server (NTRS)

    Schramm, Harry F.; Kaiser, Bruce

    2005-01-01

    A new instrument was developed for chemical characterization of surfaces that combines the analytical power of Raman spectroscopy with the three-dimensional topographic information provided by conoscopic holography. The figure schematically depicts the proposed hybrid instrument. The output of the conoscopic holographic portion of the instrument is a topographical map of the surface; the output of the Raman portion of the instrument is hyperspectral Raman data, from which the chemical and/or biological composition of the surface would be deduced. By virtue of the basic principles of design and operation of the instrument, the hyperspectral image data would be inherently spatially registered with the topographical data. In conoscopic holography, the object and reference beams of classical holography are replaced by the ordinary and extraordinary components generated by a single beam traveling through a birefringent, uniaxial crystal. In the basic conoscopic configuration, a laser light is projected onto a specimen and the resulting illuminated spot becomes a point source of diffuse light that propagates in every direction. The laser beam is rasterscanned in two dimensions (x and y) perpendicular to the beam axis (z), and at each x,y location, the pattern of interference between the ordinary and extraordinary rays is recorded. The recorded interferogram constitutes the conoscopic hologram. Of particular significance for the proposed instrument is that the conoscopic hologram contains information on the z coordinate (height) of the illuminated surface spot. Hence, a topographical map of the specimen is constructed point-by-point by rastering the laser beam in the x and y directions and correlating the x and y coordinates with the z information obtained from the interferograms. Conoscopic imaging is an established method, and conoscopic laboratory instruments for surface metrology are commercially available. In Raman spectroscopy of a surface, one measures the spectrum

  14. When holography meets coherent diffraction imaging.

    PubMed

    Latychevskaia, Tatiana; Longchamp, Jean-Nicolas; Fink, Hans-Werner

    2012-12-17

    The phase problem is inherent to crystallographic, astronomical and optical imaging where only the intensity of the scattered signal is detected and the phase information is lost and must somehow be recovered to reconstruct the object's structure. Modern imaging techniques at the molecular scale rely on utilizing novel coherent light sources like X-ray free electron lasers for the ultimate goal of visualizing such objects as individual biomolecules rather than crystals. Here, unlike in the case of crystals where structures can be solved by model building and phase refinement, the phase distribution of the wave scattered by an individual molecule must directly be recovered. There are two well-known solutions to the phase problem: holography and coherent diffraction imaging (CDI). Both techniques have their pros and cons. In holography, the reconstruction of the scattered complex-valued object wave is directly provided by a well-defined reference wave that must cover the entire detector area which often is an experimental challenge. CDI provides the highest possible, only wavelength limited, resolution, but the phase recovery is an iterative process which requires some pre-defined information about the object and whose outcome is not always uniquely-defined. Moreover, the diffraction patterns must be recorded under oversampling conditions, a pre-requisite to be able to solve the phase problem. Here, we report how holography and CDI can be merged into one superior technique: holographic coherent diffraction imaging (HCDI). An inline hologram can be recorded by employing a modified CDI experimental scheme. We demonstrate that the amplitude of the Fourier transform of an inline hologram is related to the complex-valued visibility, thus providing information on both, the amplitude and the phase of the scattered wave in the plane of the diffraction pattern. With the phase information available, the condition of oversampling the diffraction patterns can be relaxed, and the

  15. When holography meets coherent diffraction imaging.

    PubMed

    Latychevskaia, Tatiana; Longchamp, Jean-Nicolas; Fink, Hans-Werner

    2012-12-17

    The phase problem is inherent to crystallographic, astronomical and optical imaging where only the intensity of the scattered signal is detected and the phase information is lost and must somehow be recovered to reconstruct the object's structure. Modern imaging techniques at the molecular scale rely on utilizing novel coherent light sources like X-ray free electron lasers for the ultimate goal of visualizing such objects as individual biomolecules rather than crystals. Here, unlike in the case of crystals where structures can be solved by model building and phase refinement, the phase distribution of the wave scattered by an individual molecule must directly be recovered. There are two well-known solutions to the phase problem: holography and coherent diffraction imaging (CDI). Both techniques have their pros and cons. In holography, the reconstruction of the scattered complex-valued object wave is directly provided by a well-defined reference wave that must cover the entire detector area which often is an experimental challenge. CDI provides the highest possible, only wavelength limited, resolution, but the phase recovery is an iterative process which requires some pre-defined information about the object and whose outcome is not always uniquely-defined. Moreover, the diffraction patterns must be recorded under oversampling conditions, a pre-requisite to be able to solve the phase problem. Here, we report how holography and CDI can be merged into one superior technique: holographic coherent diffraction imaging (HCDI). An inline hologram can be recorded by employing a modified CDI experimental scheme. We demonstrate that the amplitude of the Fourier transform of an inline hologram is related to the complex-valued visibility, thus providing information on both, the amplitude and the phase of the scattered wave in the plane of the diffraction pattern. With the phase information available, the condition of oversampling the diffraction patterns can be relaxed, and the

  16. Combined optimal quantization and lossless coding of digital holograms of three-dimensional objects

    NASA Astrophysics Data System (ADS)

    Shortt, Alison E.; Naughton, Thomas J.; Javidi, Bahram

    2006-10-01

    Digital holography is an inherently three-dimensional (3D) technique for the capture of real-world objects. Many existing 3D imaging and processing techniques are based on the explicit combination of several 2D perspectives (or light stripes, etc.) through digital image processing. The advantage of recording a hologram is that multiple 2D perspectives can be optically combined in parallel, and in a constant number of steps independent of the hologram size. Although holography and its capabilities have been known for many decades, it is only very recently that digital holography has been practically investigated due to the recent development of megapixel digital sensors with sufficient spatial resolution and dynamic range. The applications of digital holography could include 3D television, virtual reality, and medical imaging. If these applications are realized, compression standards will have to be defined. We outline the techniques that have been proposed to date for the compression of digital hologram data and show that they are comparable to the performance of what in communication theory is known as optimal signal quantization. We adapt the optimal signal quantization technique to complex-valued 2D signals. The technique relies on knowledge of the histograms of real and imaginary values in the digital holograms. Our digital holograms of 3D objects are captured using phase-shift interferometry. We complete the compression procedure by applying lossless techniques to the quantized holographic pixels.

  17. Holographic fluorescence microscopy with incoherent digital holographic adaptive optics.

    PubMed

    Jang, Changwon; Kim, Jonghyun; Clark, David C; Lee, Seungjae; Lee, Byoungho; Kim, Myung K

    2015-01-01

    Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: a wavefront sensor, wavefront corrector, and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, for example, lenslet arrays for sensing or multiactuator deformable mirrors for correcting. We have previously introduced an alternate approach based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile are possible not only with conventional coherent digital holography, but also with a new type of digital holography using incoherent light: selfinterference incoherent digital holography (SIDH). The SIDH generates a complex—i.e., amplitude plus phase—hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. Adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media.

  18. Holographic fluorescence microscopy with incoherent digital holographic adaptive optics.

    PubMed

    Jang, Changwon; Kim, Jonghyun; Clark, David C; Lee, Seungjae; Lee, Byoungho; Kim, Myung K

    2015-01-01

    Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: a wavefront sensor, wavefront corrector, and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, for example, lenslet arrays for sensing or multiactuator deformable mirrors for correcting. We have previously introduced an alternate approach based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile are possible not only with conventional coherent digital holography, but also with a new type of digital holography using incoherent light: selfinterference incoherent digital holography (SIDH). The SIDH generates a complex—i.e., amplitude plus phase—hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. Adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media. PMID:26146767

  19. Holographic fluorescence microscopy with incoherent digital holographic adaptive optics

    NASA Astrophysics Data System (ADS)

    Jang, Changwon; Kim, Jonghyun; Clark, David C.; Lee, Seungjae; Lee, Byoungho; Kim, Myung K.

    2015-11-01

    Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: a wavefront sensor, wavefront corrector, and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, for example, lenslet arrays for sensing or multiactuator deformable mirrors for correcting. We have previously introduced an alternate approach based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile are possible not only with conventional coherent digital holography, but also with a new type of digital holography using incoherent light: self­interference incoherent digital holography (SIDH). The SIDH generates a complex-i.e., amplitude plus phase-hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. Adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media.

  20. X-ray holography in-flight

    NASA Astrophysics Data System (ADS)

    Gorkhover, Tais; Ulmer, Anatoli; Ferguson, Ken; Bucher, Max; Ekeberg, Tomas; Hantke, Max; Daurer, Benedikt; Nettelblad, Carl; Bielecki, Johan; Faigel, Guila; Hasse, Dirk; Morgan, Andrew; Mühlig, Kerstin; Seibert, Marvin; Chapman, Henry; Hajdu, Janos; Maia, Filipe; Moeller, Thomas; Bostedt, Christoph

    2016-05-01

    The advent of X-ray free-electron lasers, delivering ultra intense femtosecond X-ray flashes, opens the door for structure determination of single nanoparticles and biosamples with single shots. The first X-ray diffraction imaging experiments at LCLS delivered promising results on samples in the gas phase. However, the reconstruction of non-periodic structures is still challenging due to the loss of phase information. Meanwhile, X-ray holographic approaches allow for recording the phase directly into the diffraction image. In my talk, I will present the first successful proof-of-principle experiment for ``in-flight''-holography with free viruses. Our experiments pave the way for unique studies on levitating nanospecimen that are of central interest in several scientific communities including atmosphere research, chemistry, material sciences, and studies on matter under extreme conditions.

  1. Adaptive holography for optical sensing applications

    NASA Astrophysics Data System (ADS)

    Residori, S.; Bortolozzo, U.; Peigné, A.; Molin, S.; Nouchi, P.; Dolfi, D.; Huignard, J. P.

    2016-03-01

    Adaptive holography is a promising method for high sensitivity phase modulation measurements in the presence of slow perturbations from the environment. The technique is based on the use of a nonlinear recombining medium, here an optically addressed spatial light modulator specifically realized to operate at 1.55 μm. Owing to the physical mechanisms involved, the interferometer adapts to slow phase variations within a range of 5-10 Hz, thus filtering out low frequency noise while transmitting higher frequency phase modulations. We present the basic principles of the adaptive interferometer and show that it can be used in association with a sensing fiber in order to detect phase modulations. Finally, a phase-OTDR architecture using the adaptive holographic interferometer is presented and shown to allows the detection of localized perturbations along the sensing fiber.

  2. Quantum probabilities for inflation from holography

    SciTech Connect

    Hartle, James B.; Hawking, S.W.; Hertog, Thomas E-mail: S.W.Hawking@damtp.cam.ac.uk

    2014-01-01

    The evolution of the universe is determined by its quantum state. The wave function of the universe obeys the constraints of general relativity and in particular the Wheeler-DeWitt equation (WDWE). For non-zero Λ, we show that solutions of the WDWE at large volume have two domains in which geometries and fields are asymptotically real. In one the histories are Euclidean asymptotically anti-de Sitter, in the other they are Lorentzian asymptotically classical de Sitter. Further, the universal complex semiclassical asymptotic structure of solutions of the WDWE implies that the leading order in h-bar quantum probabilities for classical, asymptotically de Sitter histories can be obtained from the action of asymptotically anti-de Sitter configurations. This leads to a promising, universal connection between quantum cosmology and holography.

  3. Industrial Holography - The Rolls-Royce Experience

    NASA Astrophysics Data System (ADS)

    Parker, R. J.; Jones, D. G.

    1987-10-01

    Holographic interferometry has provided the engineer with one of the most powerful and versatile measurement tools of the twentieth century. It seems only natural that such a technique should have found widespread application in the aero-engine industry. The quest for ever more fuel-efficient gas-turbine engines is constantly stretching and extending the frontiers of technology. It creates an increasing need for understanding of the mechanical and aerodynamic behaviour of components under extreme conditions. Holography has recently achieved new importance as a means of providing the data necessary for the development and validation of computer models. Increasingly, design work is being performed by the application of complicated three-dimensional computer models to gas flow prediction and the use of finite-element models for the investigation of mechanical behaviour and stress.

  4. Experimental research of digital holographic microscopic measuring

    NASA Astrophysics Data System (ADS)

    Zhu, Xueliang; Chen, Feifei; Li, Jicheng

    2013-06-01

    Digital holography is a new imaging technique, which is developed on the base of optical holography, Digital processing, and Computer techniques. It is using CCD instead of the conventional silver to record hologram, and then reproducing the 3D contour of the object by the way of computer simulation. Compared with the traditional optical holographic, the whole process is of simple measuring, lower production cost, faster the imaging speed, and with the advantages of non-contact real-time measurement. At present, it can be used in the fields of the morphology detection of tiny objects, micro deformation analysis, and biological cells shape measurement. It is one of the research hot spot at home and abroad. This paper introduced the basic principles and relevant theories about the optical holography and Digital holography, and researched the basic questions which influence the reproduce images in the process of recording and reconstructing of the digital holographic microcopy. In order to get a clear digital hologram, by analyzing the optical system structure, we discussed the recording distance and of the hologram. On the base of the theoretical studies, we established a measurement and analyzed the experimental conditions, then adjusted them to the system. To achieve a precise measurement of tiny object in three-dimension, we measured MEMS micro device for example, and obtained the reproduction three-dimensional contour, realized the three dimensional profile measurement of tiny object. According to the experiment results consider: analysis the reference factors between the zero-order term and a pair of twin-images by the choice of the object light and the reference light and the distance of the recording and reconstructing and the characteristics of reconstruction light on the measurement, the measurement errors were analyzed. The research result shows that the device owns certain reliability.

  5. Random phase-free computer holography and its applications

    NASA Astrophysics Data System (ADS)

    Shimobaba, Tomoyoshi; Kakue, Takashi; Ito, Tomoyoshi

    2016-06-01

    Random phase is required in computer-generated hologram (CGH) to widely diffuse object light and to avoid its concentration on the CGH; however, the random phase causes considerable speckle noise in the reconstructed image and degrades the image quality. We introduce a simple and computationally inexpensive method that improves the image quality and reduces the speckle noise by multiplying the object light with the designed convergence light. We furthermore propose the improved method of the designed convergence light with iterative method to reduce ringing artifacts. Subsequently, as the application, a lensless zoomable holographic projection is introduced.

  6. Digital holographic Michelson interferometer for nanometrology

    NASA Astrophysics Data System (ADS)

    Sevrygin, Alexander A.; Korotkov, V. I.; Pulkin, S. A.; Tursunov, I. M.; Venediktov, D. V.; Venediktov, V. Yu.; Volkov, O. V.

    2014-11-01

    The paper considers the dynamic holographic interferometry schemes with amplification (multiplication) of holographic fringes and with correction for distortions, imposed by the interferometer scheme elements. The use of digital microscope and of the matrix light modulator with direct addressing provides the completely digital closed-loop performance of the overall system for real-time evaluation of nano-scale objects size. Considered schemes were verified in the laboratory experiment, using the Michelson micro-interferometer, equipped by the USB-microscope and digital holography stage, equipped by the Holoeye spatial light modulator.

  7. Basic Principles of Solar Acoustic Holography - (Invited Review)

    NASA Astrophysics Data System (ADS)

    Lindsey, C.; Braun, D. C.

    2000-03-01

    We summarize the basic principles of holographic seismic imaging of the solar interior, drawing on familiar principles in optics and parallels with standard optical holography. Computational seismic holography is accomplished by the phase-coherent wave-mechanical reconstruction of the p-mode acoustic field into the solar interior based on helioseismic observations at the solar surface. It treats the acoustic field at the solar surface in a way broadly analogous to how the eye treats electromagnetic radiation at the surface of the cornea, wave-mechanically refocusing radiation from submerged sources to render stigmatic images that can be sampled over focal surfaces at any desired depth. Holographic diagnostics offer a straight-forward assessment of the informational content of the observed p-mode spectrum independent of prospective physical models of the local interior anomalies that it represents. Computational holography was proposed as the optimum approach whereby to address the severe diffraction effects that confront standard tomography in the solar p-mode environment. It has given us a number of remarkable discoveries in the last two years and now promises a new insight into solar interior structure and dynamics in the local perspective. We compare the diagnostic roles of simple acoustic-power holography and phase-sensitive holography, and anticipate approaches to solar interior modeling based on holographic signatures. We identify simple computational principles that, applied to high-quality helioseismic observations, make it easy for prospective analysts to produce high-quality holographic images for practical applications in local helioseismology.

  8. Compressive sensing sectional imaging for single-shot in-line self-interference incoherent holography

    NASA Astrophysics Data System (ADS)

    Weng, Jiawen; Clark, David C.; Kim, Myung K.

    2016-05-01

    A numerical reconstruction method based on compressive sensing (CS) for self-interference incoherent digital holography (SIDH) is proposed to achieve sectional imaging by single-shot in-line self-interference incoherent hologram. The sensing operator is built up based on the physical mechanism of SIDH according to CS theory, and a recovery algorithm is employed for image restoration. Numerical simulation and experimental studies employing LEDs as discrete point-sources and resolution targets as extended sources are performed to demonstrate the feasibility and validity of the method. The intensity distribution and the axial resolution along the propagation direction of SIDH by angular spectrum method (ASM) and by CS are discussed. The analysis result shows that compared to ASM the reconstruction by CS can improve the axial resolution of SIDH, and achieve sectional imaging. The proposed method may be useful to 3D analysis of dynamic systems.

  9. Practical holography IV; Proceedings of the Meeting, LOS Angeles, CA, Jan. 18, 19, 1990

    NASA Astrophysics Data System (ADS)

    Benton, Stephen A.

    1990-05-01

    Various papers on practical holography are presented. Individual topics addressed include: dichromated gelatin for holographic optical elements, novel enhancement of photopolymers, photopolymers for holography, new rehalogenating bleach for the production of Lippmann holograms, effects of bleach constituents on the performance of silver-halide holograms, highly sensitive positive resists for holography, holographic 3-D printer, generation of high-quality holograms with liquid-crystal SLM, display holography for medical tomography, 3-D display of ultrasound B-mode image by holographic stereogram, edge-lit rainbow holograms, electronic display system for computational holography, and DCG recording with red light. Also discussed are: corneal topography via two-wavelength holography, optically produced cylindrical HOEs for signal-processing applications, hybrid diffractive-refractive telescope, volume IR reflective gratings, holographic lithography for microcircuits, photorefractive waveguide grating switches for optical interconnects, non-Fourier computer-generated holography for 3-D display, phase selection in binary-phase-only filters for optical pattern recognition.

  10. Cave Holography - Out of the lab and under the ground

    NASA Astrophysics Data System (ADS)

    Klayer, J.

    2013-02-01

    This paper describes the combination of my hobbies, caving and holography. Most traditional holography involves bringing the objects to a lab with all the necessary holography equipment mounted on a stable table. I instead bring all the equipment assembled as a portable unit to the natural formations in a cave with the cave itself being the stable table. The first successes were Denisyuks made with a HeNe or laser diode and spatial filter mounted on a tripod. For greater depth, transmission holograms were made with a DPSS laser in several configurations sometimes using fiber optics to route the reference beam and sometimes a spatial filter and mirrors. The cave environment presents unique obstacles that have been overcome as evidenced by the beautiful holograms made.

  11. Pit Distribution Design for Computer-Generated Waveguide Holography

    NASA Astrophysics Data System (ADS)

    Yagi, Shogo; Imai, Tadayuki; Ueno, Masahiro; Ohtani, Yoshimitsu; Endo, Masahiro; Kurokawa, Yoshiaki; Yoshikawa, Hiroshi; Watanabe, Toshifumi; Fukuda, Makoto

    2008-02-01

    Multilayered waveguide holography (MWH) is one of a number of page-oriented data multiplexing holographies that will be applied to optical data storage and three-dimensional (3D) moving images. While conventional volumetric holography using photopolymer or photorefractive materials requires page-by-page light exposure for recording, MWH media can be made by employing stamping and laminating technologies that are suitable for mass production. This makes devising an economical mastering technique for replicating holograms a key issue. In this paper, we discuss an approach to pit distribution design that enables us to replace expensive electron beam mastering with economical laser beam mastering. We propose an algorithm that avoids the overlapping of even comparatively large adjacent pits when we employ laser beam mastering. We also compensate for the angular dependence of the diffraction power, which strongly depends on pit shape, by introducing an enhancement profile so that a diffracted image has uniform intensity.

  12. Shallow depth subsurface imaging with microwave holography

    NASA Astrophysics Data System (ADS)

    Zhuravlev, Andrei; Ivashov, Sergey; Razevig, Vladimir; Vasiliev, Igor; Bechtel, Timothy

    2014-05-01

    In this paper, microwave holography is considered as a tool to obtain high resolution images of shallowly buried objects. Signal acquisition is performed at multiple frequencies on a grid using a two-dimensional mechanical scanner moving a single transceiver over an area of interest in close proximity to the surface. The described FFT-based reconstruction technique is used to obtain a stack of plan view images each using only one selected frequency from the operating waveband of the radar. The extent of a synthetically-formed aperture and the signal wavelength define the plan view resolution, which at sounding frequencies near 7 GHz amounts to 2 cm. The system has a short depth of focus which allows easy selection of proper focusing plane. The small distance from the buried objects to the antenna does not prevent recording of clean images due to multiple reflections (as happens with impulse radars). The description of the system hardware and signal processing technique is illustrated using experiments conducted in dry sand. The microwave images of inert anti-personnel mines are demonstrated as examples. The images allow target discrimination based on the same visually-discernible small features that a human observer would employ. The demonstrated technology shows promise for modification to meet the specific practical needs required for humanitarian demining or in multi-sensor survey systems.

  13. m-Learning and holography: Compatible techniques?

    NASA Astrophysics Data System (ADS)

    Calvo, Maria L.

    2014-07-01

    Since the last decades, cell phones have become increasingly popular and are nowadays ubiquitous. New generations of cell phones are now equipped with text messaging, internet, and camera features. They are now making their way into the classroom. This is creating a new teaching and learning technique, the so called m-Learning (or mobile-Learning). Because of the many benefits that cell phones offer, teachers could easily use them as a teaching and learning tool. However, an additional work from the teachers for introducing their students into the m-Learning in the classroom needs to be defined and developed. As an example, optical techniques, based upon interference and diffraction phenomena, such as holography, appear to be convenient topics for m-Learning. They can be approached with simple examples and experiments within the cell phones performances and classroom accessibility. We will present some results carried out at the Faculty of Physical Sciences in UCM to obtain very simple holographic recordings via cell phones. The activities were carried out inside the course on Optical Coherence and Laser, offered to students in the fourth course of the Grade in Physical Sciences. Some open conclusions and proposals will be presented.

  14. Holography And Holometry Applications In Dental Research

    NASA Astrophysics Data System (ADS)

    Willenborg, George C.

    1987-06-01

    The earliest reference to holographic applications appeared in the dental literature in 1972 when Wictorin, Bjelkhagen and Abramson described a method to study elastic deformation of defective gold solder joints in simulated fixed bridges. Their paper, published in the Swedish dental literature, offered a concise presentation of the interferometry technique which led to the development of other research applications of holographic interferometry(holometry) in dentistry. In this presentation, the development and application of the interferometry technique in the dental field will be discussed. Various interesting and potentially useful applications of holography have appeared in the dental literature over the past decade. Some of these, which will be discussed, include the use of holograms as a storage medium for dental study models, multiplexing of computer(CT) scan sections to form white light viewable holograms and the potential application of holographic training aids in the teaching of the basic courses of dental anatomy and restorative dentistry. In addition, some unique related applications will be mentioned including a laser reflection method for accurate non-contact measurement of tooth mobility/movement and a technique for contour mapping of occlusal surfaces to measure wear of restorative materials.

  15. A simplified holography based superresolution system

    NASA Astrophysics Data System (ADS)

    Mudassar, Asloob Ahmad

    2015-12-01

    In this paper we are proposing a simple idea based on holography to achieve superresolution. The object is illuminated by three fibers which maintain the mutual coherence between the light waves. The object in-plane rotation along with fiber-based illumination is used to achieve superresolution. The object in a 4f optical system is illuminated by an on-axis fiber to make the central part of the object's spectrum to the pass through the limiting square-aperture placed at the Fourier plane and the corresponding hologram of the image is recorded at the image plane. The on-axis fiber is switched off and the two off axis fibers (one positioned on the vertical axis and the other positioned on diagonal) are switched on one by one for each orientation of the object position. Four orientations of object in-plane rotation are used differing in angle by 90°. This will allow the recording of eight holographic images in addition to the one recorded with on-axis fiber. The three fibers are at the vertices of a right angled isosceles triangle and are aligned toward the centre of the lens following the fiber plane to generate plane waves for object illumination. The nine holographic images are processed for construction of object's original spectrum, the inverse of which gives the super-resolved image of the original object. Mathematical modeling and simulations are reported.

  16. Acoustic-emission linear-pulse holography

    SciTech Connect

    Collins, H.D.; Lemon, D.K.; Busse, L.J.

    1982-06-01

    This paper describes Acoustic Emission Linear Pulse Holography which combines the advantages of linear imaging and acoustic emission into a single NDE inspection system. This unique system produces a chronological linear holographic image of a flaw by utilizing the acoustic energy emitted during crack growth. Conventional linear holographic imaging uses an ultrasonic transducer to transmit energy into the volume being imaged. When the crack or defect reflects that energy, the crack acts as a new source of acoustic waves. To formulate an image of that source, a receiving transducer is scanned over the volume of interest and the phase of the received signals is measured at successive points on the scan. The innovation proposed here is the utilization of the crack generated acoustic emission as the acoustic source and generation of a line image of the crack as it grows. A thirty-two point sampling array is used to construct phase-only linear holograms of simulated acoustic emission sources on large metal plates. The phases are calculated using the pulse time-of-flight (TOF) times from the reference transducer to the array of receivers. Computer reconstruction of the image is accomplished using a one-dimensional FFT algorithm (i.e., backward wave). Experimental results are shown which graphically illustrate the unique acoustic emission images of a single point and a linear crack in a 100 mm x 1220 mm x 1220 mm aluminum plate.

  17. Holography and the virtual patient: the holographic medical image

    NASA Astrophysics Data System (ADS)

    Ko, Kathryn; Erickson, Ronald R.; Webster, John M.

    1996-12-01

    Practical holographic systems utilizing the pulsed laser are finding potential applications in medicine. Exploiting both the hologram's true 3D image and holographic interferometry these techniques enhance the physician's vision beyond the 2D radiological imaging of even the best CT and MRI. The authors describe the use of pulsed laser holography as applied to the morphological specialties: anatomy, pathology, and surgery. The authors report on the Holographic Brain Anatomy Atlas for medical education; pathologic documentation with holography, and the use of holographic interferometry in surgical planning. The techniques are outlined and a discussion on the interpretation of holographic interferometry with living subjects is provided.

  18. Probing Molecular Dynamics by Laser-Induced Backscattering Holography.

    PubMed

    Haertelt, Marko; Bian, Xue-Bin; Spanner, Michael; Staudte, André; Corkum, Paul B

    2016-04-01

    We use differential holography to overcome the forward scattering problem in strong-field photoelectron holography. Our differential holograms of H_{2} and D_{2} molecules exhibit a fishbonelike structure, which arises from the backscattered part of the recolliding photoelectron wave packet. We demonstrate that the backscattering hologram can resolve the different nuclear dynamics between H_{2} and D_{2} with subangstrom spatial and subcycle temporal resolution. In addition, we show that attosecond electron dynamics can be resolved. These results open a new avenue for ultrafast studies of molecular dynamics in small molecules. PMID:27081975

  19. Probing Molecular Dynamics by Laser-Induced Backscattering Holography

    NASA Astrophysics Data System (ADS)

    Haertelt, Marko; Bian, Xue-Bin; Spanner, Michael; Staudte, André; Corkum, Paul B.

    2016-04-01

    We use differential holography to overcome the forward scattering problem in strong-field photoelectron holography. Our differential holograms of H2 and D2 molecules exhibit a fishbonelike structure, which arises from the backscattered part of the recolliding photoelectron wave packet. We demonstrate that the backscattering hologram can resolve the different nuclear dynamics between H2 and D2 with subangstrom spatial and subcycle temporal resolution. In addition, we show that attosecond electron dynamics can be resolved. These results open a new avenue for ultrafast studies of molecular dynamics in small molecules.

  20. Resolution and quality enhancement in terahertz in-line holography by sub-pixel sampling with double-distance reconstruction.

    PubMed

    Li, Zeyu; Li, Lei; Qin, Yu; Li, Guangbin; Wang, Du; Zhou, Xun

    2016-09-01

    We demonstrate the enhancement of resolution and image quality in terahertz (THz) lens-free in-line digital holography by sub-pixel sampling with double-distance reconstruction. Multiple sub-pixel shifted low-resolution (LR) holograms recorded by a pyroelectric array detector (100 μm × 100 μm pixel pitch, 124 × 124 pixels) are aligned precisely to synthesize a high-resolution (HR) hologram. By this method, the lateral resolution is no more limited by the pixel pitch, and lateral resolution of 150 μm is obtained, which corresponds to 1.26λ with respect to the illuminating wavelength of 118.8 μm (2.52 THz). Compared with other published works, to date, this is the highest resolution in THz digital holography when considering the illuminating wavelength. In addition, to suppress the twin-image and zero-order artifacts, the complex amplitude distributions of both object and illuminaing background wave fields are reconstructed simultaneously. This is achieved by iterative phase retrieval between the double HR holograms and background images at two recording planes, which does not require any constraints on object plane or a priori knowledge of the sample. PMID:27607716

  1. Comparison of Thermal Detector Arrays for Off-Axis THz Holography and Real-Time THz Imaging.

    PubMed

    Hack, Erwin; Valzania, Lorenzo; Gäumann, Gregory; Shalaby, Mostafa; Hauri, Christoph P; Zolliker, Peter

    2016-01-01

    In terahertz (THz) materials science, imaging by scanning prevails when low power THz sources are used. However, the application of array detectors operating with high power THz sources is increasingly reported. We compare the imaging properties of four different array detectors that are able to record THz radiation directly. Two micro-bolometer arrays are designed for infrared imaging in the 8-14 μm wavelength range, but are based on different absorber materials (i) vanadium oxide; (ii) amorphous silicon; (iii) a micro-bolometer array optimized for recording THz radiation based on silicon nitride; and (iv) a pyroelectric array detector for THz beam profile measurements. THz wavelengths of 96.5 μm, 118.8 μm, and 393.6 μm from a powerful far infrared laser were used to assess the technical performance in terms of signal to noise ratio, detector response and detectivity. The usefulness of the detectors for beam profiling and digital holography is assessed. Finally, the potential and limitation for real-time digital holography are discussed. PMID:26861341

  2. Comparison of Thermal Detector Arrays for Off-Axis THz Holography and Real-Time THz Imaging.

    PubMed

    Hack, Erwin; Valzania, Lorenzo; Gäumann, Gregory; Shalaby, Mostafa; Hauri, Christoph P; Zolliker, Peter

    2016-02-06

    In terahertz (THz) materials science, imaging by scanning prevails when low power THz sources are used. However, the application of array detectors operating with high power THz sources is increasingly reported. We compare the imaging properties of four different array detectors that are able to record THz radiation directly. Two micro-bolometer arrays are designed for infrared imaging in the 8-14 μm wavelength range, but are based on different absorber materials (i) vanadium oxide; (ii) amorphous silicon; (iii) a micro-bolometer array optimized for recording THz radiation based on silicon nitride; and (iv) a pyroelectric array detector for THz beam profile measurements. THz wavelengths of 96.5 μm, 118.8 μm, and 393.6 μm from a powerful far infrared laser were used to assess the technical performance in terms of signal to noise ratio, detector response and detectivity. The usefulness of the detectors for beam profiling and digital holography is assessed. Finally, the potential and limitation for real-time digital holography are discussed.

  3. Comparison of Thermal Detector Arrays for Off-Axis THz Holography and Real-Time THz Imaging

    PubMed Central

    Hack, Erwin; Valzania, Lorenzo; Gäumann, Gregory; Shalaby, Mostafa; Hauri, Christoph P.; Zolliker, Peter

    2016-01-01

    In terahertz (THz) materials science, imaging by scanning prevails when low power THz sources are used. However, the application of array detectors operating with high power THz sources is increasingly reported. We compare the imaging properties of four different array detectors that are able to record THz radiation directly. Two micro-bolometer arrays are designed for infrared imaging in the 8–14 μm wavelength range, but are based on different absorber materials (i) vanadium oxide; (ii) amorphous silicon; (iii) a micro-bolometer array optimized for recording THz radiation based on silicon nitride; and (iv) a pyroelectric array detector for THz beam profile measurements. THz wavelengths of 96.5 μm, 118.8 μm, and 393.6 μm from a powerful far infrared laser were used to assess the technical performance in terms of signal to noise ratio, detector response and detectivity. The usefulness of the detectors for beam profiling and digital holography is assessed. Finally, the potential and limitation for real-time digital holography are discussed. PMID:26861341

  4. In situ micro-focused X-ray beam characterization with a lensless camera using a hybrid pixel detector.

    PubMed

    Kachatkou, Anton; Marchal, Julien; van Silfhout, Roelof

    2014-03-01

    Results of studies on micro-focused X-ray beam diagnostics using an X-ray beam imaging (XBI) instrument based on the idea of recording radiation scattered from a thin foil of a low-Z material with a lensless camera are reported. The XBI instrument captures magnified images of the scattering region within the foil as illuminated by the incident beam. These images contain information about beam size, beam position and beam intensity that is extracted during dedicated signal processing steps. In this work the use of the device with beams for which the beam size is significantly smaller than that of a single detector pixel is explored. The performance of the XBI device equipped with a state-of-the-art hybrid pixel X-ray imaging sensor is analysed. Compared with traditional methods such as slit edge or wire scanners, the XBI micro-focused beam characterization is significantly faster and does not interfere with on-going experiments. The challenges associated with measuring micrometre-sized beams are described and ways of optimizing the resolution of beam position and size measurements of the XBI instrument are discussed.

  5. Optical interference with digital holograms

    NASA Astrophysics Data System (ADS)

    Gossman, David; Perez-Garcia, Benjamin; Hernandez-Aranda, Raul I.; Forbes, Andrew

    2016-07-01

    In 1804, Thomas Young reported the observation of fringes in the intensity of light, and attributed it to the concept of interference between coherent sources. In this paper, we revisit this famous experiment and show how it can easily be demonstrated with digital holography. We look closely at the concept of interference with light and ask, "fringes in what?" We then show that depending on how light interferes, fringe patterns in observables other than intensity can be seen. We explain this conceptually and demonstrate it experimentally. We provide a holistic approach to the topic, aided by modern laboratory practices for a straightforward demonstration of the underlying physics.

  6. Single-shot dual-polarization holography: measurement of the polarization state of a magnetic sample

    NASA Astrophysics Data System (ADS)

    Khodadad, Davood; Amer, Eynas; Gren, Per; Melander, Emil; Hällstig, Emil; Sjödahl, Mikael

    2015-08-01

    In this paper a single-shot digital holographic set-up with two orthogonally polarized reference beams is proposed to achieve rapid acquisition of Magneto-Optical Kerr Effect images. Principles of the method and the background theory for dynamic state of polarization measurement by use of digital holography are presented. This system has no mechanically moving elements or active elements for polarization control and modulation. An object beam is combined with two reference beams at different off-axis angles and is guided to a detector. Then two complex fields (interference terms) representing two orthogonal polarizations are recorded in a single frame simultaneously. Thereafter the complex fields are numerically reconstructed and carrier frequency calibration is done to remove aberrations introduced in multiplexed digital holographic recordings. From the numerical values of amplitude and phase, a real time quantitative analysis of the polarization state is possible by use of Jones vectors. The technique is demonstrated on a magnetic sample that is a lithographically patterned magnetic microstructure consisting of thin permalloy parallel stripes.

  7. Spaceflight Holography Investigation in a Virtual Apparatus

    NASA Technical Reports Server (NTRS)

    2003-01-01

    The movement of a particle in a fluid is one of the most fundamental processes in physics and plays an important role in materials science. For example, the physics of crystal growth from a nucleation site in solution can be dominated by movement of the solution over the crystal surface. Gravity usually dominates the equations of motion, but in microgravity other terms can dominate, making the equation much more complex. Until recently, the equations were solved only by numerical methods and/or by neglecting terms. During this study, we discovered an exact solution to the equations, which shows that the usually neglected terms become extremely important in microgravity. We also developed diagnostic recording methods using holography to save all of the particle field data, allowing the experiment to essentially be transferred from space back to earth in what we call the virtual apparatus . We will record holograms of particle distributions in motion in microgravity and bring them back to earth for analysis, allowing the study of the full three-dimensional motion of sets of particles, allowing us to test the new analytical solutions. The experiment will also provide accurate measurements of the quasi-steady acceleration of the space platform and other interesting microgravity and g-jitter effects. This program will produce the flight definition for an experiment in the microgravity environment of space to validate the theoretical model. We will design an experiment with the help of the theoretical model that is optimized for testing the model, measuring g, g-jitter, and other microgravity phenomena.

  8. Intraoperative brain tumor resection cavity characterization with conoscopic holography

    NASA Astrophysics Data System (ADS)

    Simpson, Amber L.; Burgner, Jessica; Chen, Ishita; Pheiffer, Thomas S.; Sun, Kay; Thompson, Reid C.; Webster, Robert J., III; Miga, Michael I.

    2012-02-01

    Brain shift compromises the accuracy of neurosurgical image-guided interventions if not corrected by either intraoperative imaging or computational modeling. The latter requires intraoperative sparse measurements for constraining and driving model-based compensation strategies. Conoscopic holography, an interferometric technique that measures the distance of a laser light illuminated surface point from a fixed laser source, was recently proposed for non-contact surface data acquisition in image-guided surgery and is used here for validation of our modeling strategies. In this contribution, we use this inexpensive, hand-held conoscopic holography device for intraoperative validation of our computational modeling approach to correcting for brain shift. Laser range scan, instrument swabbing, and conoscopic holography data sets were collected from two patients undergoing brain tumor resection therapy at Vanderbilt University Medical Center. The results of our study indicate that conoscopic holography is a promising method for surface acquisition since it requires no contact with delicate tissues and can characterize the extents of structures within confined spaces. We demonstrate that for two clinical cases, the acquired conoprobe points align with our model-updated images better than the uncorrected images lending further evidence that computational modeling approaches improve the accuracy of image-guided surgical interventions in the presence of soft tissue deformations.

  9. Intellectual property issues in holography and high tech

    NASA Astrophysics Data System (ADS)

    Reingand, Nadya

    2004-06-01

    The author with technical education background (Ph.D. in holography) shares her 3+ years of experience working on intellectual property (IP) issues that includes patents, trademarks, and copyrights. A special attention is paid to the patent issues: the application procedure, the patent requirements, the databases for prior art search, how to make the cost efficient filing.

  10. Conoscopic holography for image registration: a feasibility study

    NASA Astrophysics Data System (ADS)

    Lathrop, Ray A.; Cheng, Tiffany T.; Webster, Robert J., III

    2009-02-01

    Preoperative image data can facilitate intrasurgical guidance by revealing interior features of opaque tissues, provided image data can be accurately registered to the physical patient. Registration is challenging in organs that are deformable and lack features suitable for use as alignment fiducials (e.g. liver, kidneys, etc.). However, provided intraoperative sensing of surface contours can be accomplished, a variety of rigid and deformable 3D surface registration techniques become applicable. In this paper, we evaluate the feasibility of conoscopic holography as a new method to sense organ surface shape. We also describe potential advantages of conoscopic holography, including the promise of replacing open surgery with a laparoscopic approach. Our feasibility study investigated use of a tracked off-the-shelf conoscopic holography unit to perform a surface scans on several types of biological and synthetic phantom tissues. After first exploring baseline accuracy and repeatability of distance measurements, we performed a number of surface scan experiments on the phantom and ex vivo tissues with a variety of surface properties and shapes. These indicate that conoscopic holography is capable of generating surface point clouds of at least comparable (and perhaps eventually improved) accuracy in comparison to published experimental laser triangulation-based surface scanning results.

  11. Video-rate laser Doppler vibrometry by heterodyne holography.

    PubMed

    Samson, Benjamin; Verpillat, Frédéric; Gross, Michel; Atlan, Michael

    2011-04-15

    We report a demonstration video-rate heterodyne holography in off-axis configuration. Reconstruction and display of a one megapixel hologram is achieved at 24 frames per second, with a graphics processing unit. Our claims are validated with real-time screening of steady-state vibration amplitudes in a wide-field, noncontact vibrometry experiment.

  12. Calendar effects in quantum mechanics in view of interactive holography

    NASA Astrophysics Data System (ADS)

    Berkovich, Simon

    2013-04-01

    Quantum mechanics in terms of interactive holography appears as `normal' science [1]. With the holography quantum behavior is determined by the interplay of material formations and their conjugate images. To begin with, this effortlessly elucidates the nonlocality in quantum entanglements. Then, it has been shown that Schr"odinger's dynamics for a single particle arises from Bi-Fragmental random walks of the particle itself and its holographic image. For many particles this picture blurs with fragments merging as bosons or fermions. In biomolecules, swapping of particles and their holographic placeholders leads to self-replication of the living matter. Because of broad interpretations of quantum formalism direct experiments attributing it to holography may not be very compelling. The holographic mechanism better reveals as an absolute frame of reference. A number of physical and biological events exhibit annual variations when Earth orbital position changes with respect to the universal holographic mechanism. The well established calendar variations of heart attacks can be regarded as a positive outcome of a generalization of the Michelson experiment, where holography is interferometry and ailing hearts are detectors of pathologically replicated proteins. Also, there have been already observed calendar changes in radioactive decay rates. The same could be expected for various fine quantum experiences, like, e.g., Josephson tunneling. In other words, Quantum Mechanics (February) Quantum Mechanics (August). [1] S. Berkovich, ``A comprehensive explanation of quantum mechanics,'' www.cs.gwu.edu/research/technical-report/170 .

  13. Applications of holography. Citations from the NTIS data base

    NASA Astrophysics Data System (ADS)

    Carrigan, B.

    1980-05-01

    The bibliography covers studies on the applications of holography in such areas as photograping high-speed particles, nondestructive testing of material defects, strain analysis, microscopy, interferometry, vibration measurement, and medical diagnosis. This updated bibliography contains 249 abstracts, none of which are new entries to the previous edition.

  14. Applications of holography. Citations from the NTIS data base

    NASA Astrophysics Data System (ADS)

    Carrigan, B.

    1980-05-01

    The bibliography covers studies on the applications of holography in such areas as photographing high-speed particles, nondestructive testing of material defects, strain analysis, microscopy, interferometry, vibration measurement, and medical diagnosis. This updated bibliography contains 59 abstracts, 42 of which are new entries to the previous edition.

  15. Applications of PIV and Holography to Characterize Flow and Particle Distributions in the Ocean

    NASA Astrophysics Data System (ADS)

    Katz, J.; Nayak, A. R.; Talapatra, S.; Hong, J.

    2012-12-01

    This presentation discusses in-situ applications of particle image velocimetry (PIV) and digital holography to characterize the flow and particle distributions in oceanic flows. The PIV measurements focused on the mean flow and turbulence in the inner part of the coastal ocean bottom boundary layer. The stationary submersible system recorded 2D velocity distributions in two 30x30 cm planes, the first aligned with the tidal current, and the second with the dominant wave direction. The orientation of the illuminated planes were controlled remotely and re-adjusted to maintain alignment with the current and waves. Data were transmitted via optical fibers to a ship-board acquisition system. In recent field tests, the system was deployed off New Jersey at a depth of 25 m. Data acquired at 6 Hz (12 frames/s) covered varying velocity, bottom roughness, as well as relative wave-current orientations and magnitude. Sets spanning entire tidal cycles were recorded in multiple 35-60 min subsets, each providing 12,600-21600 velocity distributions. Co-deployed ADV facilitated calculations of Reynolds stresses by filtering out the contribution of wave-induced motion, and a pencil-beam sonar was used to characterize the seabed topography. Sample profiles of mean flow and turbulence parameters will focus on the interaction of currents and waves with the bottom roughness, and will show, e.g., instabilities at the interface between wave and current boundary layers, signature of bottom roughness in the turbulent energy spectra, and variations in velocity profiles across bottom ripples. The particle distributions were measured using a free-drifting, submersible, digital holography system, which maintained a low relative velocity (<1 cm/s) with the local fluid. Consequently, images of the same particles could be observed in multiple frames, enabling us to examine their behavior, as well as directly measure the local profiles of shear strain and dissipation rates form the velocity

  16. Submersible digital holographic cameras and their application to marine science

    NASA Astrophysics Data System (ADS)

    Watson, John

    2011-09-01

    Digital holography has been growing in importance for application to environmental studies in the oceans and lakes of the world. With an imaging resolution using ``classical'' photoholography of a few micro-meters and recording volumes up to a cubic meter, several ``holocameras'' were developed and deployed for underwater imaging of plankton and other marine particles. For in-water deployment, however, the weight and size of these instruments restricted their use on advanced observation platforms such as remotely operated vehicles, and limited operational depth to a few hundred meters. Advances made in digital recording on electronic sensors, coupled with numerical reconstruction, led to the development of smaller, rugged holocameras. This freed holography from many of its constraints and allowed rapid capture and storage of images and holographic video recording of moving objects. Although holography is not the only optical method applicable underwater, its ability to record full-field, high-resolution, distortion free images in situ from which particle dimensions, distribution and dynamics can be extracted is hard to match. The current state-of-the-art in underwater holography is discussed, with an outline of some submersible holocameras. We describe one such system, eHoloCam, in more depth and present results from its deployment in the North Sea.

  17. Comparative simulations of Fresnel holography methods for atomic waveguides

    NASA Astrophysics Data System (ADS)

    Henderson, V. A.; Griffin, P. F.; Riis, E.; Arnold, A. S.

    2016-02-01

    We have simulated the optical properties of micro-fabricated Fresnel zone plates (FZPs) as an alternative to spatial light modulators for producing non-trivial light potentials to trap atoms within a lensless Fresnel arrangement. We show that binary (1 bit) FZPs with wavelength (1 μm) spatial resolution consistently outperform kinoforms of spatial and phase resolution comparable to commercial SLMs in root mean square error comparisons, with FZP kinoforms demonstrating increasing improvement for complex target intensity distributions. Moreover, as sub-wavelength resolution microfabrication is possible, FZPs provide an exciting possibility for the creation of static cold-atom trapping potentials useful to atomtronics, interferometry, and the study of fundamental physics.

  18. Optical holography applications for the zero-g Atmospheric Cloud Physics Laboratory

    NASA Technical Reports Server (NTRS)

    Kurtz, R. L.

    1974-01-01

    A complete description of holography is provided, both for the time-dependent case of moving scene holography and for the time-independent case of stationary holography. Further, a specific holographic arrangement for application to the detection of particle size distribution in an atmospheric simulation cloud chamber. In this chamber particle growth rate is investigated; therefore, the proposed holographic system must capture continuous particle motion in real time. Such a system is described.

  19. Stimulated Raman scattering holography for time-resolved imaging of methane gas.

    PubMed

    Amer, Eynas; Gren, Per; Edenharder, Stefan; Sjödahl, Mikael

    2016-05-01

    In this paper, pulsed digital holographic detection is coupled to the stimulated Raman scattering (SRS) process for imaging gases. A Q-switched Nd-YAG laser (532 nm) has been used to pump methane gas (CH4) at pressures up to 12 bars. The frequency-tripled (355 nm) beam from the same laser was used to pump an optical parametric oscillator (OPO). The Stokes beam (from the OPO) has been tuned to 629.93 nm so that the frequency difference between the pump (532 nm) and the Stokes beams fits a Raman active vibrational mode of the methane molecule (2922  cm-1). The pump beam has been spatially modulated with fringes produced in a Michelson interferometer. The pump and the Stokes beams were overlapped in time, space, and polarization on the gas molecules, resulting in a stimulated Raman gain of the Stokes beam and a corresponding loss of the pump beam through the SRS process. The resulting gain of the Stokes beam has been detected using pulsed digital holography by blending it with a reference beam on the detector. Two holograms of the Stokes beam, without and with the pump beam fringes present, were recorded. Intensity maps calculated from the recorded digital holograms showed amplification of the Stokes beam at the position of overlap with the pump beam fringes and the gas molecules. The gain of the Stokes beam has been separated from the background in the Fourier domain. A gain of about 4.5% at a pump beam average intensity of 4  MW/cm2 and a Stokes beam intensity of 0.16  MW/cm2 have been recorded at a gas pressure of 12 bars. The gain decreased linearly with decreasing gas pressure. The results show that SRS holography is a promising technique to pinpoint a specific species and record its spatial and temporal distribution. PMID:27140351

  20. QCD and Light-Front Holography

    SciTech Connect

    Brodsky, Stanley J.; de Teramond, Guy F.; /Costa Rica U.

    2010-10-27

    The soft-wall AdS/QCD model, modified by a positive-sign dilaton metric, leads to a remarkable one-parameter description of nonperturbative hadron dynamics. The model predicts a zero-mass pion for zero-mass quarks and a Regge spectrum of linear trajectories with the same slope in the leading orbital angular momentum L of hadrons and the radial quantum number N. Light-Front Holography maps the amplitudes which are functions of the fifth dimension variable z of anti-de Sitter space to a corresponding hadron theory quantized on the light front. The resulting Lorentz-invariant relativistic light-front wave equations are functions of an invariant impact variable {zeta} which measures the separation of the quark and gluonic constituents within the hadron at equal light-front time. The result is to a semi-classical frame-independent first approximation to the spectra and light-front wavefunctions of meson and baryon light-quark bound states, which in turn predict the behavior of the pion and nucleon form factors. The theory implements chiral symmetry in a novel way: the effects of chiral symmetry breaking increase as one goes toward large interquark separation, consistent with spectroscopic data, and the the hadron eigenstates generally have components with different orbital angular momentum; e.g., the proton eigenstate in AdS/QCD with massless quarks has L = 0 and L = 1 light-front Fock components with equal probability. The soft-wall model also predicts the form of the non-perturbative effective coupling {alpha}{sub s}{sup AdS} (Q) and its {beta}-function which agrees with the effective coupling {alpha}{sub g1} extracted from the Bjorken sum rule. The AdS/QCD model can be systematically improved by using its complete orthonormal solutions to diagonalize the full QCD light-front Hamiltonian or by applying the Lippmann-Schwinger method in order to systematically include the QCD interaction terms. A new perspective on quark and gluon condensates is also reviewed.

  1. Higher-spin theory and holography

    NASA Astrophysics Data System (ADS)

    Gaberdiel, Matthias; Vasiliev, Mikhail

    2013-05-01

    , this has triggered a significant amount of activity in this general area. Among other things, the constraints that are implied by the higher-spin symmetries were analysed (see the paper by Maldacena and Zhiboedov in this issue [9]), and a fairly concrete proposal for how higher-spin theories are related to string theory was made (see the paper by Chang, Minwalla, Sharma and Yin in this issue [10]). Furthermore, a lower dimensional version of the conjecture was put forward by Gaberdiel and Gopakumar [11] that was subsequently also checked in some detail. These dualities hold the promise of offering insights into the inner workings of the AdS/CFT correspondence since they are complex enough to capture the essence of the duality, while at the same time being sufficiently simple in order to allow for a detailed analysis. Moreover, the methods specifically developed in higher-spin theory may be useful for understanding a general mechanism underlying holography, both in higher-spin models and beyond (see the paper by Vasiliev in this issue [12]). Another fascinating aspect of these higher-spin theories lies in the fact that the higher-spin symmetries mix generically fields of different spin, and in particular, the spin-2 metric and higher-spin excitations are related to one another by gauge transformations. As a result, higher-spin theories require a modification of the standard framework of Riemannian geometry since the usual diffeomorphism-invariant tensors are not gauge invariant any longer. In particular, higher-spin theories may therefore open the way towards understanding fundamental concepts of space-time geometry; for example, they may well have key lessons in store for how string theory resolves space-time singularities. In this issue we have collected together a number of review papers, summarizing the aforementioned recent developments, as well as research papers indicating current directions of interest in the study of higher-spin gauge theories. We hope that it

  2. Art-science, beauty-reason and holography

    NASA Astrophysics Data System (ADS)

    Jeong, T. H.

    2013-02-01

    Display holography holds a distinction that makes it appealing to a wide audience. It can be appreciated at a deep level by people of all ages and in all fields of endeavor. It provides a unique opportunity for us to gather in an intimate location to learn, enjoy, and enlighten one another. This paper offers demonstrations to explore the relationships between art and science, esthetics and mathematics, and the dualities that exist in nature. On the practical level, a visual model for deep understanding of holography and a proposal for "making holograms that sell" will be presented. In writing this article, the author acknowledges the fact that for this symposium, a Proceeding will be published as well as a set of audio-visual recordings. With that in mind, this article represents largely the printable contents, leaving the audio-visual part as "performance" to be electronically recorded.

  3. Local magnetic structure determination using polarized neutron holography

    SciTech Connect

    Szakál, Alex Markó, Márton Cser, László

    2015-05-07

    A unique and important property of the neutron is that it possesses magnetic moment. This property is widely used for determination of magnetic structure of crystalline samples observing the magnetic components of the diffraction peaks. Investigations of diffraction patterns give information only about the averaged structure of a crystal but for discovering of local spin arrangement around a specific (e.g., impurity) nucleus remains still a challenging problem. Neutron holography is a useful tool to investigate the local structure around a specific nucleus embedded in a crystal lattice. The method has been successfully applied experimentally in several cases using non-magnetic short range interaction of the neutron and the nucleus. A mathematical model of the hologram using interaction between magnetic moment of the atom and the neutron spin for polarized neutron holography is provided. Validity of a polarized neutron holographic experiment is demonstrated by applying the proposed method on model systems.

  4. Photoinduced anisotropy in rigid dye solutions for transient polarization holography

    NASA Astrophysics Data System (ADS)

    Todorov, T.; Nikolova, L.; Tomova, N.; Dragostinova, V.

    1986-08-01

    The photochromic properties of three rigid dyes solutions were assessed experimentally. The dyes, susceptible to induced photooptical anisotropy, are candidates as recording media for transient polarization holography. Attention is focused on the transitions from singlet to triplet states in fluorescein/orthoboric acid (FOA), isomeric transformations in methyl red/polymer matrices, and molecular reorientations in methyl orange/polyvinylalchol (MOP) in response to degenerate four-wave mixing laser hologroaphy. Various combinations of linear, orthogonal and circular polarizations were tested. All three dye/matrix systems resisted fatigue past 10,000 erasure cycles, with the FOA system exhibiting the shortest response time, 0.1 sec. The MOP mixture displayed a diffraction efficiency of nearly 35 percent. Techniques for accelerating the recording processes for using the dyes in holography to study phenomena such as energy transfer and wavefront reversal are discussed.

  5. Three-dimensional optical holography using a plasmonic metasurface

    PubMed Central

    Huang, Lingling; Chen, Xianzhong; Mühlenbernd, Holger; Zhang, Hao; Chen, Shumei; Bai, Benfeng; Tan, Qiaofeng; Jin, Guofan; Cheah, Kok-Wai; Qiu, Cheng-Wei; Li, Jensen; Zentgraf, Thomas; Zhang, Shuang

    2013-01-01

    Benefitting from the flexibility in engineering their optical response, metamaterials have been used to achieve control over the propagation of light to an unprecedented level, leading to highly unconventional and versatile optical functionalities compared with their natural counterparts. Recently, the emerging field of metasurfaces, which consist of a monolayer of photonic artificial atoms, has offered attractive functionalities for shaping wave fronts of light by introducing an abrupt interfacial phase discontinuity. Here we realize three-dimensional holography by using metasurfaces made of subwavelength metallic nanorods with spatially varying orientations. The phase discontinuity takes place when the helicity of incident circularly polarized light is reversed. As the phase can be continuously controlled in each subwavelength unit cell by the rod orientation, metasurfaces represent a new route towards high-resolution on-axis three-dimensional holograms with a wide field of view. In addition, the undesired effect of multiple diffraction orders usually accompanying holography is eliminated.

  6. Underwater visual inspection and measurement using optical holography

    NASA Astrophysics Data System (ADS)

    Watson, John

    In offshore oil and gas exploration, visual inspection and measurement often has to be carried out under conditions of poor visibility and at great depths. Recording a hologram of an underwater scene with its subsequent replay in the laboratory could provide enormous benefits to the inspection engineer. When replayed in the real image mode, dimensional measurement may be performed directly on the image formed in real space using, for example, measuring microscopy or TV cameras. Potential applications include general archiving, measurement of corrosion pitting and cracking, examination and measurement of damage sites, structural profiling and examination of marine growth. In this paper, we outline the general concepts of underwater holography. A résumé is given of the work undertaken in the past, the problems experienced and their solution and an indication of our general direction leading to the establishment of holography as a unique method of subsea inspection.

  7. X-ray holography with a customizable reference.

    PubMed

    Martin, Andrew V; D'Alfonso, Adrian J; Wang, Fenglin; Bean, Richard; Capotondi, Flavio; Kirian, Richard A; Pedersoli, Emanuele; Pedersoli, Emmanuele; Raimondi, Lorenzo; Stellato, Francesco; Yoon, Chun Hong; Chapman, Henry N

    2014-01-01

    In X-ray Fourier-transform holography, images are formed by exploiting the interference pattern between the X-rays scattered from the sample and a known reference wave. To date, this technique has only been possible with a limited set of special reference waves. We demonstrate X-ray Fourier-transform holography with an almost unrestricted choice for the reference wave, permitting experimental geometries to be designed according to the needs of each experiment and opening up new avenues to optimize signal-to-noise and resolution. The optimization of holographic references can aid the development of holographic techniques to meet the demands of resolution and fidelity required for single-shot imaging applications with X-ray lasers. PMID:25145971

  8. Positron emission zone plate holography for particle tracking

    NASA Astrophysics Data System (ADS)

    Gundogdu, O.

    2006-01-01

    Positron Emission Particle Tracking (PEPT) is a powerful non-invasive technique that has been used extensively for tracking a single particle. In this paper, we present a study of zone plate holography method in order to track multiple particles, mainly two particles. The main aim is to use as small number of events as possible in the order to make it possible to track particles in fast moving industrial systems. A zone plate with 100% focal efficiency is simulated and applied to the Positron Emission Tomography (PET) data for multiple particle tracking. A simple trajectory code was employed to explore the effects of the nature of the experimental trajectories. A computer holographic reconstruction code that simulates optical reconstruction was developed. The different aspects of the particle location, particle activity ratios for enabling tagging of particles and zone plate and hologram locations are investigated. The effect of the shot noise is investigated and the limitations of the zone plate holography are reported.

  9. Cell identification using single beam lensless imaging with pseudo-random phase encoding.

    PubMed

    Javidi, Bahram; Rawat, Siddharth; Komatsu, Satoru; Markman, Adam

    2016-08-01

    In this Letter, we propose a novel compact optical system for automated cell identification. Our system employs pseudo-random encoding of the light modulated by the cells under inspection to capture the unique opto-biological signature of the micro-organisms by an image sensor and without using a microscope objective lens to magnify the object beam. The proposed instrument can be fabricated using a compact light source, a thin diffuser, and an image sensor connected to computational hardware; thus, it can be compact and cost effective. Experiments are presented using the proposed system to identify and classify various micro-objects and demonstrate proof of concept. The captured opto-biological signature pattern can be attributed to the micro-object's morphology, size, sub-cellular complex structure, index of refraction, internal material composition, etc. Using the captured signature of the micro-object, we extract statistical features such as mean, variance, skewness, kurtosis, entropy, and correlation coefficients for cell identification using the random forest classifier. For comparison, similar identification experiments were repeated with a digital shearing interferometer. To the best of our knowledge, this is the first report on automated cell identification using the proposed approach. PMID:27472644

  10. Entanglement, Holography, and the Quantum Phases of Matter

    SciTech Connect

    Sachdev, Subir

    2012-11-07

    Electrons in many interesting materials, such as the high temperature superconductors, exhibit low energy states with complex varieties of quantum entanglement. I will describe how the methods of holography, drawn from string theory, have given us a new tool to describe such states, by relating them to theories of gravitation in curved spacetimes with an extra dimension. I will discuss the impact of such ideas on studies of quantum phase transitions, and of novel metals.

  11. Gray tone image watermarking with complementary computer generated holography.

    PubMed

    Martinez, Christophe; Laulagnet, Fabien; Lemonnier, Olivier

    2013-07-01

    We present herein an original approach for the watermarking of holograms in gray tone images for use in microscopic halftone image archiving. Our concept is based on the principle of complementary holography presented in a previous contribution. The efficiency of the concept is evaluated theoretically and experimentally. We demonstrate the interest of elliptical diffraction patterns as an alternative to the usual rectangular diffraction patterns and confirm the subsidiary role of the hologram amplitude in the hologram recovery process.

  12. Bi-local holography in the SYK model

    NASA Astrophysics Data System (ADS)

    Jevicki, Antal; Suzuki, Kenta; Yoon, Junggi

    2016-07-01

    We discuss large N rules of the Sachdev-Ye-Kitaev model and the bi-local representation of holography of this theory. This is done by establishing 1 /N Feynman rules in terms of bi-local propagators and vertices, which can be evaluated following the recent procedure of Polchinski and Rosenhaus. These rules can be interpreted as Witten type diagrams of the dual AdS theory, which we are able to define at IR fixed point and off.

  13. Bi-local holography in the SYK model

    DOE PAGESBeta

    Jevicki, Antal; Suzuki, Kenta; Yoon, Junggi

    2016-07-01

    We discuss large N rules of the Sachdev-Ye-Kitaev model and the bi-local representation of holography of this theory. This is done by establishing 1/N Feynman rules in terms of bi-local propagators and vertices, which can be evaluated following the recent procedure of Polchinski and Rosenhaus. Lastly, these rules can be interpreted as Witten type diagrams of the dual AdS theory, which we are able to define at IR fixed point and off.

  14. Real-Time Finite-Temperature Holography and its Applications

    NASA Astrophysics Data System (ADS)

    Wu, Chaolun

    This dissertation begins with a brief review of basic concepts of holography (AdS/CFT correspondence, Chapter 1). A complete prescription for computing real-time correlators in strongly-coupled conformal field theories at finite-temperature using holography is proposed and checked, and all scalar real-time 3-point correlators of the relativistic conformal field theory are computed at the tree level of dual gravity theory (Chapter 2). The causal 3-point correlators are found to have a simple structure which can be easily generalized to higher n-point correlators and higher spin operators. Then the same prescription is applied to non-relativistic holography (Chapter 3). All scalar real-time 2-point correlators and time-ordered and causal 3-point correlators of finite-temperature Schrödinger field theory are computed there. In the last two chapters, the prescription for relativistic real-time finite-temperature holography and in particular the causal 3-point correlators derived earlier, are used to study various properties of a strongly-coupled plasma, specifically that of N = 4 supersymmetric Yang-Mills field theory. By computing causal energy-stress tensor 3-point correlators analytically in the hydrodynamic regime and matching them with the holographic result, and through the use of newly developed second order Kubo formulae, all five second order transport coefficients of the relativistic conformal hydrodynamics are systematically and consistently computed (Chapter 4). Jet quenching in a strongly-coupled plasma at finite-temperature and finite-chemical potential is also studied by an analytic computation of causal R-current 3-point correlators in large momentum regime. A more detailed and comprehensive picture than previously known emerges (Chapter 5). The dependence of typical jet stopping distance on its initial energy has an exponent 1/4, rather than the 1/3 which was widely known, and the jet quenching is enhanced by introducing a chemical potential to the

  15. Holography and entropy bounds in the plane wave matrix model

    SciTech Connect

    Bousso, Raphael; Mints, Aleksey L.

    2006-06-15

    As a quantum theory of gravity, matrix theory should provide a realization of the holographic principle, in the sense that a holographic theory should contain one binary degree of freedom per Planck area. We present evidence that Bekenstein's entropy bound, which is related to area differences, is manifest in the plane wave matrix model. If holography is implemented in this way, we predict crossover behavior at strong coupling when the energy exceeds N{sup 2} in units of the mass scale.

  16. Ohya Large Holography Studio In A Huge Rock Cavern

    NASA Astrophysics Data System (ADS)

    Watanabe, K.; Hirosawa, A.; Morie, T.; Suzuki, M.; Yamashita, H.

    1988-01-01

    There is a great demand recently for a large size hologram, 2meters width for instance, in which a brand-new car, a life-size statue and sometimes a group of the them, or interior spaces could he taken. Especially, in the world of advertisement or art, there is a big demand for a large hologram. The larger hologram needs the larger space for its suitable holography. This is the report of a large underground studio built in Japan.

  17. Acoustic holography. Citations from the NTIS data base

    NASA Astrophysics Data System (ADS)

    Reed, W. E.

    1980-07-01

    Aspects of acoustic holography are covered in this bibliography of Federally funded research. Theory, equipment design, uses, and imaging techniques are presented. The applications include underwater and underground object locating, structural geology and tectonics, sonar imaging, non destructive testing, antenna radiation patterns, nuclear reactor inspection, remote sensing, and use in medical examination. This updated bibliography contains 166 citations, 15 of which are new entries to the previous edition.

  18. Direct Atom Imaging by Chemical-Sensitive Holography.

    PubMed

    Lühr, Tobias; Winkelmann, Aimo; Nolze, Gert; Krull, Dominique; Westphal, Carsten

    2016-05-11

    In order to understand the physical and chemical properties of advanced materials, functional molecular adsorbates, and protein structures, a detailed knowledge of the atomic arrangement is essential. Up to now, if subsurface structures are under investigation, only indirect methods revealed reliable results of the atoms' spatial arrangement. An alternative and direct method is three-dimensional imaging by means of holography. Holography was in fact proposed for electron waves, because of the electrons' short wavelength at easily accessible energies. Further, electron waves are ideal structure probes on an atomic length scale, because electrons have a high scattering probability even for light elements. However, holographic reconstructions of electron diffraction patterns have in the past contained severe image artifacts and were limited to at most a few tens of atoms. Here, we present a general reconstruction algorithm that leads to high-quality atomic images showing thousands of atoms. Additionally, we show that different elements can be identified by electron holography for the example of FeS2.

  19. Active phase compensation system for fiber optic holography

    NASA Technical Reports Server (NTRS)

    Mercer, Carolyn R.; Beheim, Glenn

    1988-01-01

    Fiber optic delivery systems promise to extend the application of holography to severe environments by simplifying test configurations and permitting the laser to be remotely placed in a more benign location. However, the introduction of optical fiber leads to phase stability problems. Environmental effects cause the pathlengths of the fibers to change randomly, preventing the formation of stationary interference patterns which are required for holography. An active phase control system has been designed and used with an all-fiber optical system to stabilize the phase difference between light emitted from two fibers, and to step the phase difference by 90 deg without applying any constraints on the placement of the fibers. The accuracy of the phase steps is shown to be better than 0.02 deg., and a stable phase difference can be maintained for 30 min. This system can be applied to both conventional and electro-optic holography, as well as to any system where the maintenance of an accurate phase difference between two coherent beams is required.

  20. Computer Generated Holography with Intensity-Graded Patterns

    PubMed Central

    Conti, Rossella; Assayag, Osnath; de Sars, Vincent; Guillon, Marc; Emiliani, Valentina

    2016-01-01

    Computer Generated Holography achieves patterned illumination at the sample plane through phase modulation of the laser beam at the objective back aperture. This is obtained by using liquid crystal-based spatial light modulators (LC-SLMs), which modulate the spatial phase of the incident laser beam. A variety of algorithms is employed to calculate the phase modulation masks addressed to the LC-SLM. These algorithms range from simple gratings-and-lenses to generate multiple diffraction-limited spots, to iterative Fourier-transform algorithms capable of generating arbitrary illumination shapes perfectly tailored on the base of the target contour. Applications for holographic light patterning include multi-trap optical tweezers, patterned voltage imaging and optical control of neuronal excitation using uncaging or optogenetics. These past implementations of computer generated holography used binary input profile to generate binary light distribution at the sample plane. Here we demonstrate that using graded input sources, enables generating intensity graded light patterns and extend the range of application of holographic light illumination. At first, we use intensity-graded holograms to compensate for LC-SLM position dependent diffraction efficiency or sample fluorescence inhomogeneity. Finally we show that intensity-graded holography can be used to equalize photo evoked currents from cells expressing different levels of chanelrhodopsin2 (ChR2), one of the most commonly used optogenetics light gated channels, taking into account the non-linear dependence of channel opening on incident light. PMID:27799896

  1. Holographic fluorescence microscopy with incoherent digital holographic adaptive optics

    NASA Astrophysics Data System (ADS)

    Jang, Changwon; Kim, Jonghyun; Clark, David C.; Lee, Byoungho; Kim, Myung K.

    2015-03-01

    Introduction of adaptive optics technology into astronomy and ophthalmology has made great contributions in these fields, allowing one to recover images blurred by atmospheric turbulence or aberrations of the eye. Similar adaptive optics improvement in microscopic imaging is also of interest to researchers using various techniques. Current technology of adaptive optics typically contains three key elements: wavefront sensor, wavefront corrector and controller. These hardware elements tend to be bulky, expensive, and limited in resolution, involving, e.g., lenslet arrays for sensing or multi-acuator deformable mirrors for correcting. We have previously introduced an alternate approach to adaptive optics based on unique capabilities of digital holography, namely direct access to the phase profile of an optical field and the ability to numerically manipulate the phase profile. We have also demonstrated that direct access and compensation of the phase profile is possible not only with the conventional coherent type of digital holography, but also with a new type of digital holography using incoherent light: self-interference incoherent digital holography (SIDH). The SIDH generates complex - i.e. amplitude plus phase - hologram from one or several interferograms acquired with incoherent light, such as LEDs, lamps, sunlight, or fluorescence. The complex point spread function can be measured using a guide star illumination and it allows deterministic deconvolution of the full-field image. We present experimental demonstration of aberration compensation in holographic fluorescence microscopy using SIDH. The adaptive optics by SIDH provides new tools for improved cellular fluorescence microscopy through intact tissue layers or other types of aberrant media.

  2. Heterodyne holography with full control of both the signal and reference arms.

    PubMed

    Gross, Michel

    2016-01-20

    Heterodyne holography is a variant of phase-shifting holography in which the reference and signal arms are controlled by acousto-optic modulators. In this review paper, we will briefly describe the method and its properties, and we will illustrate its advantages in experimental applications. PMID:26835961

  3. Analytical study of acousto/optical holography-interfacing methods for acoustical and optical holography NDT research

    NASA Technical Reports Server (NTRS)

    El-Sum, H. M. A.

    1976-01-01

    The international status of the art of acousto optical imaging techniques adaptable to nondestructive testing and, interfacing methods for acoustical and optical holography in nondestructive testing research are studied. Evaluation of 20 different techniques encompassed investigation of varieties of detectors and detection schemes, all of which are described and summarized. Related investigation is reported in an Appendix. Important remarks on image quality, factors to be considered in designing a particular system, and conclusions and recommendations are presented. Three bibliographies are included.

  4. Nonlinearity compensation and complex-to-phase conversion of complex incoherent digital holograms for optical reconstruction.

    PubMed

    Liu, Jung-Ping; Wang, Sheng-Yen; Tsang, P W M; Poon, Ting-Chung

    2016-06-27

    Incoherent digital holography (IDH) can be realized by optical scanning holography or self-interference incoherent holography. Although IDH can exhibit high quality reconstruction due to its inherently speckle-free property, direct display of an incoherent hologram is a challenge because of its amplitude nonlinearity and the demand of complex modulation. In this paper we propose to compensate the amplitude nonlinearity at the object plane, and use bidirectional error-diffusion method to convert the complex-type incoherent Fresnel hologram to a phase-only Fresnel hologram for display. A spatial light modulator is used to reconstruct the phase-only hologram optically to demonstrate the validity of our proposed method. PMID:27410610

  5. Two-photon fluorescence imaging through multicore fiber with digital phase conjugation

    NASA Astrophysics Data System (ADS)

    Stasio, Nicolino; Conkey, Donald B.; Moser, Christophe; Psaltis, Demetri

    2016-03-01

    We present near diffraction limited two photon fluorescence (TPF) imaging through a lensless, multi-core fiber (MCF) endoscope utilizing digital phase conjugation. The ultra-small size of MCFs make them desirable tools for imaging deep into the body. TPF imaging enables optical sectioning and is widely used in brain and biological imaging and is a desired modality for fiber endoscopes. Previous implementations of TPF imaging through MCFs focus and scan the light from individual cores for image formation. In such systems the resolution is limited by the MCF core spacing, although a lens may be used to improve the resolution at the expense of the field of view. Other, more recent work has improved the resolution limitation using custom built MCFs for focusing and scanning of ultrafast pulses using wavefront shaping. Here we present digital phase conjugation for ultrafast pulse focusing through a MCF for an imaging resolution independent of the MCF core spacing. Furthermore, the phase conjugation technique does not require the use of a lens at the fiber end for focus formation and is compatible with commercially available MCFs with a large number of cores. Here, we present a 3000 core MCF endoscope and demonstrate ultrafast pulse focusing with sufficient focus spot contrast and power for TPF endoscope imaging. We construct TPF images by digital scanning of the phase conjugated focus on the target object and collection of the emitted fluorescence through the MCF. This work demonstrates the viability of digital conjugation combined with commercially available MCFs for higher resolution lensless, two photon endoscopy.

  6. Applications of holography in the millimeter-wave and terahertz region

    NASA Astrophysics Data System (ADS)

    McAuley, I.; Murphy, J. A.; Trappe, N.; Mahon, R.; McCarthy, D.; McLaughlin, P.

    2011-02-01

    In this paper we report on the improvements in holographic techniques developed for applications in the millimeter-wave and terahertz range of the electromagnetic spectrum. An experimental arrangement, adapted from off-axis near-field holography at visible wavelengths, was employed that utilizes a raster scanning detector to record the holograms digitally. The object and reference fields were based on the beams from a pair of radiating antennas fed by a single coherent source via a cross-guide coupler. Using phase retrieval methods, the recorded holographic interference pattern can be used to determine the effective phase centers of radiating feed antennas, including non standard radiators such as planar lens antennas. By numerically propagating the recovered object beam back to the source plane the object beam in the vicinity of the waist (the effective phase center) can be recovered. Among the issues investigated was improvement in the accuracy of the phase retrieval process by taking account of the non-perfect reference beam. The technique has also been applied to the investigation of increased co-polarisation levels in the scattering of radiation from surface features of dielectric materials on millimeter-wave radiation.

  7. Single-shot speckle reduction in numerical reconstruction of digitally recorded holograms: comment.

    PubMed

    Maycock, Jonathan; Hennelly, Bryan; McDonald, John

    2015-09-01

    We comment on a recent Letter by Hincapie et al. [Opt. Lett.40, 1623 (2015)], in which the authors proposed a method to reduce the speckle noise in digital holograms. This method was previously published by us in Maycock ["Improving reconstructions of digital holograms," Ph.D. thesis (National University of Ireland, 2012)] and Maycock and Hennelly [Improving Reconstructions of Digital Holograms: Speckle Reduction and Occlusions in Digital Holography (Lambert Academic, 2014)]. We also wish to highlight an important limitation of the method resulting from the superposition of different perspectives of the object/scene, which was not addressed in their Letter. PMID:26368684

  8. Virtual mask digital electron beam lithography

    DOEpatents

    Baylor, L.R.; Thomas, C.E.; Voelkl, E.; Moore, J.A.; Simpson, M.L.; Paulus, M.J.

    1999-04-06

    Systems and methods for direct-to-digital holography are described. An apparatus includes a laser; a beamsplitter optically coupled to the laser; a reference beam mirror optically coupled to the beamsplitter; an object optically coupled to the beamsplitter, a focusing lens optically coupled to both the reference beam mirror and the object; and a digital recorder optically coupled to the focusing lens. A reference beam is incident upon the reference beam mirror at a non-normal angle, and the reference beam and an object beam are focused by the focusing lens at a focal plane of the digital recorder to form an image. The systems and methods provide advantages in that computer assisted holographic measurements can be made. 5 figs.

  9. Virtual mask digital electron beam lithography

    DOEpatents

    Baylor, Larry R.; Thomas, Clarence E.; Voelkl, Edgar; Moore, James A.; Simpson, Michael L.; Paulus, Michael J.

    1999-01-01

    Systems and methods for direct-to-digital holography are described. An apparatus includes a laser; a beamsplitter optically coupled to the laser; a reference beam mirror optically coupled to the beamsplitter; an object optically coupled to the beamsplitter, a focusing lens optically coupled to both the reference beam mirror and the object; and a digital recorder optically coupled to the focusing lens. A reference beam is incident upon the reference beam mirror at a non-normal angle, and the reference beam and an object beam are focused by the focusing lens at a focal plane of the digital recorder to form an image. The systems and methods provide advantages in that computer assisted holographic measurements can be made.

  10. Nonlinear volume holography for wave-front engineering.

    PubMed

    Hong, Xu-Hao; Yang, Bo; Zhang, Chao; Qin, Yi-Qiang; Zhu, Yong-Yuan

    2014-10-17

    The concept of volume holography is applied to the design of an optical superlattice for the nonlinear harmonic generation. The generated harmonic wave can be considered as a holographic image caused by the incident fundamental wave. Compared with the conventional quasi-phase-matching method, this new method has significant advantages when applied to complicated nonlinear processes such as the nonlinear generation of special beams. As an example, we experimentally realized a second-harmonic Airy beam, and the results are found to agree well with numerical simulations.

  11. Interior near-field acoustical holography in flight.

    PubMed

    Williams, E G; Houston, B H; Herdic, P C; Raveendra, S T; Gardner, B

    2000-10-01

    In this paper boundary element methods (BEM) are mated with near-field acoustical holography (NAH) in order to determine the normal velocity over a large area of a fuselage of a turboprop airplane from a measurement of the pressure (hologram) on a concentric surface in the interior of the aircraft. This work represents the first time NAH has been applied in situ, in-flight. The normal fuselage velocity was successfully reconstructed at the blade passage frequency (BPF) of the propeller and its first two harmonics. This reconstructed velocity reveals structure-borne and airborne sound-transmission paths from the engine to the interior space.

  12. Beamforming and holography image formation methods: an analytic study.

    PubMed

    Solimene, Raffaele; Cuccaro, Antonio; Ruvio, Giuseppe; Tapia, Daniel Flores; O'Halloran, Martin

    2016-04-18

    Beamforming and holographic imaging procedures are widely used in many applications such as radar sensing, sonar, and in the area of microwave medical imaging. Nevertheless, an analytical comparison of the methods has not been done. In this paper, the Point Spread Functions pertaining to the two methods are analytically determined. This allows a formal comparison of the two techniques, and to easily highlight how the performance depends on the configuration parameters, including frequency range, number of scatterers, and data discretization. It is demonstrated that the beamforming and holography basically achieve the same resolution but beamforming requires a cheaper (less sensors) configuration.. PMID:27137336

  13. What are the ideal wavelengths for full color holography?

    NASA Astrophysics Data System (ADS)

    Bazargan, Kaveh

    2013-02-01

    One of the holy grails in display holography is the production of natural color holographic images. Various sets of wavelengths for recording have been suggested, some favoring three wavelengths, some four, and even more. I will argue that the choice of recording wavelengths is completely independent of the holographic process; it was in fact was solved once and for all by scientists working in general lighting in the 1970s. I will suggest an ideal set of wavelengths which will produce color rendition equal to better than conventional photographic processes.

  14. Beamforming and holography image formation methods: an analytic study.

    PubMed

    Solimene, Raffaele; Cuccaro, Antonio; Ruvio, Giuseppe; Tapia, Daniel Flores; O'Halloran, Martin

    2016-04-18

    Beamforming and holographic imaging procedures are widely used in many applications such as radar sensing, sonar, and in the area of microwave medical imaging. Nevertheless, an analytical comparison of the methods has not been done. In this paper, the Point Spread Functions pertaining to the two methods are analytically determined. This allows a formal comparison of the two techniques, and to easily highlight how the performance depends on the configuration parameters, including frequency range, number of scatterers, and data discretization. It is demonstrated that the beamforming and holography basically achieve the same resolution but beamforming requires a cheaper (less sensors) configuration..

  15. Criteria for the observation of strong-field photoelectron holography

    SciTech Connect

    Marchenko, T.; Huismans, Y.; Schafer, K. J.; Vrakking, M. J. J.

    2011-11-15

    Photoelectron holography is studied experimentally and computationally using the ionization of ground-state xenon atoms by intense near-infrared radiation. A strong dependence of the occurrence of the holographic pattern on the laser wavelength and intensity is observed, and it is shown that the observation of the hologram requires that the ponderomotive energy U{sub p} is substantially larger than the photon energy. The holographic interference is therefore favored by longer wavelengths and higher laser intensities. Our results indicate that the tunneling regime is not a necessary condition for the observation of the holographic pattern, which can be observed under the conditions formally attributed to the multiphoton regime.

  16. Dual-channel in-line digital holographic double random phase encryption

    PubMed Central

    Das, Bhargab; Yelleswarapu, Chandra S; Rao, D V G L N

    2012-01-01

    We present a robust encryption method for the encoding of 2D/3D objects using digital holography and virtual optics. Using our recently developed dual-plane in-line digital holography technique, two in-line digital holograms are recorded at two different planes and are encrypted using two different double random phase encryption configurations, independently. The process of using two mutually exclusive encryption channels makes the system more robust against attacks since both the channels should be decrypted accurately in order to get a recognizable reconstruction. Results show that the reconstructed object is unrecognizable even when the portion of the correct phase keys used during decryption is close to 75%. The system is verified against blind decryptions by evaluating the SNR and MSE. Validation of the proposed method and sensitivities of the associated parameters are quantitatively analyzed and illustrated. PMID:23471012

  17. Dual-channel in-line digital holographic double random phase encryption.

    PubMed

    Das, Bhargab; Yelleswarapu, Chandra S; Rao, D V G L N

    2012-10-01

    We present a robust encryption method for the encoding of 2D/3D objects using digital holography and virtual optics. Using our recently developed dual-plane in-line digital holography technique, two in-line digital holograms are recorded at two different planes and are encrypted using two different double random phase encryption configurations, independently. The process of using two mutually exclusive encryption channels makes the system more robust against attacks since both the channels should be decrypted accurately in order to get a recognizable reconstruction. Results show that the reconstructed object is unrecognizable even when the portion of the correct phase keys used during decryption is close to 75%. The system is verified against blind decryptions by evaluating the SNR and MSE. Validation of the proposed method and sensitivities of the associated parameters are quantitatively analyzed and illustrated.

  18. 3D measurement of the position of gold particles via evanescent digital holographic particle tracking velocimetry

    NASA Astrophysics Data System (ADS)

    Satake, Shin-ichi; Unno, Noriyuki; Nakata, Shuichiro; Taniguchi, Jun

    2016-08-01

    A new technique based on digital holography and evanescent waves was developed for 3D measurements of the position of gold nanoparticles in water. In this technique, an intensity profile is taken from a holographic image of a gold particle. To detect the position of the gold particle with high accuracy, its holographic image is recorded on a nanosized step made of MEXFLON, which has a refractive index close to that of water, and the position of the particle is reconstructed by means of digital holography. The height of the nanosized step was measured by using a profilometer and the digitally reconstructed height of the glass substrate had good agreement with the measured value. Furthermore, this method can be used to accurately track the 3D position of a gold particle in water.

  19. The application of holography as a real-time three-dimensional motion picture camera

    NASA Technical Reports Server (NTRS)

    Kurtz, R. L.

    1973-01-01

    A historical introduction to holography is presented, as well as a basic description of sideband holography for stationary objects. A brief theoretical development of both time-dependent and time-independent holography is also provided, along with an analytical and intuitive discussion of a unique holographic arrangement which allows the resolution of front surface detail from an object moving at high speeds. As an application of such a system, a real-time three-dimensional motion picture camera system is discussed and the results of a recent demonstration of the world's first true three-dimensional motion picture are given.

  20. Cylindrical acoustical holography applied to full-scale jet noise.

    PubMed

    Wall, Alan T; Gee, Kent L; Neilsen, Tracianne B; Krueger, David W; James, Michael M

    2014-09-01

    Near-field acoustical holography methods are used to predict sound radiation from an engine installed on a high-performance military fighter aircraft. Cylindrical holography techniques are an efficient approach to measure the large and complex sound fields produced by full-scale jets. It is shown that a ground-based, one-dimensional array of microphones can be used in conjunction with a cylindrical wave function field representation to provide a holographic reconstruction of the radiated sound field at low frequencies. In the current work, partial field decomposition methods and numerical extrapolation of data beyond the boundaries of the hologram aperture are required prior to holographic projection. Predicted jet noise source distributions and directionality are shown for four frequencies between 63 and 250 Hz. It is shown that the source distribution narrows and moves upstream, and that radiation directionality shifts toward the forward direction, with increasing frequency. A double-lobe feature of full-scale jet radiation is also demonstrated. PMID:25190387

  1. 25 Years Of Holography The Development Of Holographic Testing

    NASA Astrophysics Data System (ADS)

    Rottenkolber, H.

    1988-06-01

    It was of course Dennis Gabor who, as long ago as 1948, published the fundamental work the principle of holography. But it was not until Theodore Maiman's invention of the laser in 1960 that the practical realisation of holography became possible. This was achieved by Leith and Upatnicks between 1962 and 1964, with the crucial impetus being provided by Vander Lugt (the author wishes to acknowledge the assistence of A. Vander Lugt, who, while performing work of a relatet nature, showed him the value of the gas laser as a light source). 1964 was the year in which Denisyuk presented his first portrait holograms in Leningrad. The many publications of Stroke of the University of New York were a source of numerous impulses. It is to this period, too, that my own first experiences date back. Together with my teacher. Ulrich Grigull, whom I hold in the highest regard, I constructed the first laser interferometer for the study of heat transfer and developed the initial ideas for holo-graphic interferometry. Then, in 1965, came the work of Hildebrandt and Haines and of Powell and Stetson in the United States in this field, and it was around this time, too, that Nassenstein and his colleagues Riek and Bestenreiner began their work in this new area in Germany.

  2. Poor man’s holography: how far can it go?

    NASA Astrophysics Data System (ADS)

    Tian, Yu; Wu, Xiao-Ning; Zhang, Hongbao

    2013-06-01

    Almost a century ago, Einstein, after Newton, shed new light on gravity by claiming that gravity is geometry. There has been no deeper insight beyond that later on except the recent suspicion that gravity may also be holographic, dual to some sort of quantum field theory living on the boundary with one less dimension. Such a suspicion has been supported mainly by a variety of specific examples from string theory. This paper is intended to purport the holographic gravity from a different perspective. Namely, we shall show that such a holography can actually be observed by working merely within the context of Einstein’s gravity through promoting Brown-York’s formalism, where neither is the spacetime required to be asymptotically AdS nor the boundary to be located at conformal infinity, which also conforms to the spirit inherited from Wilson’s effective field theory. In particular, we show that our holography works remarkably well at least at the level of thermodynamics and hydrodynamics, where a perfect matching between the bulk gravity and boundary fluid is found for entropy and its production by the conserved current method.

  3. Holography Applied To Inspection And Mensuration In An Industrial Environment

    NASA Astrophysics Data System (ADS)

    Tozer, B. A.; Glanville, R.; Gordon, A. L.; Little, M. J.; Webster, J. M.; Wright, D. G.

    1985-06-01

    The three dimensional imaging capability combined with enormous information storage capacity and the related very high resolution of images when they are correctly reconstructed, combine to make holography an inspection tool of immense power and utility in capital intensive industries. This paper describes work, in the U.K. electricity generating industry, aimed at enabling high grade holograms to be routinely recorded in a wide range of industrial situations where high speed acquisition of visual data is desirable. Specific applications to be described are holography of nuclear reactor cores, and of irradiated fuel elements for inspection after withdrawal from the reactor. Accurate reconstruction of images and precise, three dimensional measurement of reconstructed images in a laboratory environment is then undertaken with computer controlled micromanipulator equipment traversing a television camera within the real image. Measurements of a metre long fuel element to within 0.1 mm have been demonstrated and resolutions of 50 1.p./mm have been obtained on the reconstructed object. The paper describes the engineering required for in-reactor inspection where the pulsed ruby laser used to record the holograms is routinely alignec to a 15 m beam relay and remote holographic head. The requirements for accurate 3-dimensional reconstruction and hologrammetry are described and plans for automated measurement and inspection using image enhancement techniques are discussed.

  4. Color holography: a new technique for reproduction of paintings

    NASA Astrophysics Data System (ADS)

    Bjelkhagen, Hans I.; Vukicevic, Dalibor

    2002-06-01

    Until recently, display holography was usually associate with 3D imaging. After the appearance of color holography it has become possible, however, to record holographic images of 2D objects, such as, for example, oil paintings. The realistic-looking virtual image recorded in a Denisyuk reflection hologram is the most suitable for such reproductions. A holographic contact recording of a painting reproduces the painting with all its texture details preserved, such as brush strokes, the painter's signature, etc. This means that an exact copy of the painting can be made, which can then be displayed at art exhibitions, museums, etc., when the original is not available. If an expensive painting is concerned, possessing an exact copy of the painting may also be important for insurance purposes, in case the painting is stolen or damaged. The advantage of a color contact hologram is that the hologram reconstruction process can be relaxed, as there is no need of spatial coherence of the white light source used to illuminate the hologram. In addition, no depth distortions are introduced as a function of the light source's distance from the plate. Only the angel of illumination is of primary importance if good color reproduction is to be obtained. The paper discusses the rendition of color in hologram, which is extremely important in this case. The holographic reproduction process of an oil painting is also described, and the major advantages of holographic reproduction are discussed together with its limitations.

  5. The implementation of holography in the plane wave matrix model

    NASA Astrophysics Data System (ADS)

    Mints, Aleksey Leonidovich

    It is expected that at the core of nonperturbative theories of quantum gravity, such as M-theory, lies the realization of the holographic principle, in the sense that a holographic theory should contain one binary degree of freedom per Planck area. Present understanding of such theories requires the holographic encoding of bulk data in large matrices. Currently this mapping is poorly understood. The plane wave matrix model provides a laboratory for isolating aspects of this problem in a controlled setting. At large boosts, configurations of concentric membranes become superselection sectors, whose exact spectra are known. From the bulk point of view one expects product states of individual membranes to be contained within the full spectrum. However, for non-BPS states this inclusion relation is obscured by Gauss law constraints. Its validity rests on nontrivial relations in representation theory, which we identify and verify by explicit computation. Beyond the decoding and partial identification of selected states in large matrices, one would like to get a better understanding of the holographic state counting of these degrees of freedom, i.e., entropy. Contrary to the naive expectation of holography realized in terms of the covariant entropy bound, we present evidence that it is the Bekenstein entropy bound, which is related to area differences, that is manifest in the plane wave matrix model. If holography is implemented in this way, we predict crossover behavior at strong coupling when the energy exceeds N2 in units of the mass scale.

  6. SHIVA-(Spaceflight Holography in a Virtual Apparatus)

    NASA Technical Reports Server (NTRS)

    Trolinger, James D.; LaI, Ravindra B.; Rangel, Roger; Coimbra, Carlos; Witherow William; Rogers, Jan

    2001-01-01

    SHIVA (Spaceflight Holography Investigation in a Virtual Apparatus) will expand our understanding of the fundamental physics of particle movement in fluids by exploiting the power of holography in a spaceflight experiment'. In addition, the study will exploit the movement of particles in fluids to observe and quantify microgravity phenomena that are extremely important in materials sciences with applications both in space and on earth. The regime under scrutiny is the low Reynolds number, Stokes regime or creeping flow, which covers particles and bubbles moving at very low velocity. The equations describing this important regime have been under development and investigation for over 100 years and yet a complete analytical solution of the general equation had remained elusive yielding only approximations and numerical solutions. In the course of the ongoing NASA NRA, the first analytical solution of the general equation was produced by members of the investigator team using the mathematics of fractional derivatives. This opened the way to an even more insightful and important investigation of the phenomena in microgravity.

  7. Real-time numerical reconstruction of digitally recorded holograms

    NASA Astrophysics Data System (ADS)

    Trujillo, Carlos; Restrepo, John F.; Garcia-Sucerquia, J.

    2011-08-01

    The numerical reconstruction of digitally recorded holograms has constituted the bottle neck for real-time digital holography. The reconstruction process can be understood as the diffraction that undergoes a wavefront as it illuminates the digitally recorded hologram. As this process is done numerically, the reconstruction of a M × N pixels hologram into an image of similar dimensions is an operation with a Ο (M × N)2 complexity. The diffraction process can be represented by a Fresnel transform or a scalable convolution of the recorded hologram. In these representations the numerical reconstruction has a complexity of Ο (M × log N)2, still quite demanding computationally if the holograms are of 2048 × 2048 pixels. In this work, the power provided by a Graphics Processing Unit (GPU) is used to accelerate the numerical reconstruction of digitally recorded holograms. The methodology is supported on the parallelization of typical Fresnel transform and scalable reconstruction algorithms. On reconstructing holograms of 2048 × 2048 pixels, the reconstruction is speeded up 20 times for the former method and 11 times for the scalable convolution. For holograms of 1024 × 1024, the accelerated reconstruction methods allow for real-time digital holography.

  8. Haptic Holography: Acoustic Space and the Evolution of the Whole Message

    NASA Astrophysics Data System (ADS)

    Logan, N.

    2013-02-01

    The paper argues that the Haptic Holography Work Station is an example of a medium that fit's with McLuhan's notion of Acoustic Space, that is it is a medium which stimulates more than one sense of perception at a time. As a result, the Haptic Holography Work Station transmits information about the subject much more rapidly than other media that precedes it, be it text, photography or television.

  9. Phase and fluorescence imaging by combination of digital holographic microscopy and fluorescence microscopy

    NASA Astrophysics Data System (ADS)

    Quan, Xiangyu; Nitta, Kouichi; Matoba, Osamu; Xia, Peng; Awatsuji, Yasuhiro

    2015-04-01

    Hybrid digital holographic microscopy that combines fluorescence microscopy and digital holographic microscopy into a single system for biological applications is proposed. In the proposed system, a phase image and a fluorescence image can be obtained simultaneously by selecting the different wavelengths of the fluorescent light and the phase measurement. Especially for biological applications, the cell structure can be obtained by the phase imaging based on digital holography and nucleus of the cell can be obtained by the fluorescence imaging. The measurement of fluorescence beads and egera densa presented the feasibility of simultaneous detection of both a phase image and a fluorescence image.

  10. Estimation of spectral transmittance curves from RGB images in color digital holographic microscopy using speckle illuminations

    NASA Astrophysics Data System (ADS)

    Funamizu, Hideki; Tokuno, Yuta; Aizu, Yoshihisa

    2016-06-01

    We investigate the estimation of spectral transmittance curves in color digital holographic microscopy using speckle illuminations. In color digital holography, it has the disadvantage in that the color-composite image gives poor color information due to the use of lasers with the two or three wavelengths. To overcome this disadvantage, the Wiener estimation method and an averaging process using multiple holograms are applied to color digital holographic microscopy. Estimated spectral transmittance and color-composite images are shown to indicate the usefulness of the proposed method.

  11. Holography demonstrations and workshops for science and engineering outreach

    NASA Astrophysics Data System (ADS)

    Thomas, Weston; Kruse, Kevin; Middlebrook, Christopher

    2012-10-01

    The SPIE/OSA Student Chapter at Michigan Technological University have developed demonstrations and workshops for science and engineering outreach. The practical approach to holography promotes the study of photonic related sciences in high school and college-aged students. An introduction to laser safety, optical laboratory practices, and basic laser coherence theory is given in order to first introduce the participants to the science behind the holograms. The students are then able to create a hologram of an item of their choice, personalizing the experience. By engaging directly, the students are able to see how the theory is applied and also enforces a higher level of attention from them so no mistakes are made in their hologram. Throughout the course participants gain an appreciation for photonics by learning how holograms operate and are constructed through hands on creation of their own holograms. This paper reviews the procedures and methods used in the demonstrations and workshop while examining the overall student experience.

  12. High resolution integral holography using Fourier ptychographic approach.

    PubMed

    Li, Zhaohui; Zhang, Jianqi; Wang, Xiaorui; Liu, Delian

    2014-12-29

    An innovative approach is proposed for calculating high resolution computer generated integral holograms by using the Fourier Ptychographic (FP) algorithm. The approach initializes a high resolution complex hologram with a random guess, and then stitches together low resolution multi-view images, synthesized from the elemental images captured by integral imaging (II), to recover the high resolution hologram through an iterative retrieval with FP constrains. This paper begins with an analysis of the principle of hologram synthesis from multi-projections, followed by an accurate determination of the constrains required in the Fourier ptychographic integral-holography (FPIH). Next, the procedure of the approach is described in detail. Finally, optical reconstructions are performed and the results are demonstrated. Theoretical analysis and experiments show that our proposed approach can reconstruct 3D scenes with high resolution.

  13. Gordon Research Conference on Holography and Optical Information Processing

    NASA Astrophysics Data System (ADS)

    Walkup, John F.

    1991-09-01

    The 1991 Gordon Research Conference on Holography and Optical Information Processing was held on 17-21 June, 1991 at Plymouth State College in Plymouth, NH. The talks on Ultrafast Optical Logic Using Solitons and Optical Processing with Planar Optics pointed out the significant progress being made by various Bell Labs investigators on various aspects of optical computing. Professor Sing Lee from the University of California-San Diego gave an excellent overview of some engineering and performance Issues in optoelectronic computing. It is clear that the Soviets have made considerable progress on multi transducer acousto-optic spectrum analyzers. It is also clear that the Japanese government is planning to continue to make optical computing one of its high priority areas, with its NIPT (New Information Processing Technology project), and are projected to continue to investigate so called sixth generation computer technologies.

  14. Indefinite Plasmonic Beam Engineering by In-plane Holography

    NASA Astrophysics Data System (ADS)

    Chen, J.; Li, L.; Li, T.; Zhu, S. N.

    2016-06-01

    Recent advances in controlling the optical phase at the sub-wavelength scale by meta-structures offer unprecedented possibilities in the beam engineering, holograms, and even invisible cloaks. In despite of developments of plasmonic beam engineering for definite beams, here, we proposed a new holographic strategy by in-plane diffraction process to access indefinite plasmonic beams, where a counterintuitive oscillating beam was achieved at a free metal surface that is against the common recognition of light traveling. Beyond the conventional hologram, our approach emphasizes on the phase correlation on the target, and casts an in-depth insight into the beam formation as a kind of long depth-of-field object. Moreover, in contrast to previous plasmonic holography with space light as references, our approach is totally fulfilled in a planar dimension that offers a thoroughly compact manipulation of the plasmonic near-field and suggests new possibilities in nanophotonic designs.

  15. A Study on Applications of Holography in Solar Energy Installations

    NASA Astrophysics Data System (ADS)

    Abbasi, Hamed; Granmayeh Rad, Adeleh; Zarei, Talie; Jalali Farahani, Neda

    In this paper, applications of holography in the solar energy, photovoltaic concentration, daylighting, illumination, and thermal blocking have been investigated. Holographic elements can be used to concentrate the radiation of the sun onto photovoltaic cells. Moreover the sun radiation is diffracted by the hologram. This has the advantage that proper photovoltaic cells can be installed in different spectral regions. Holographic daylighting systems can diffract sunlight efficiently up to the ceiling in the room. Holograms can be fabricated to reflect or block certain wavelength regions. Holograms are designed for a wavelength in the infrared region and a typical incidence angle of the summer sunlight. In this case the infrared radiation is reflected in the summer and heating of the rooms can be reduced, also, energy for cooling by air conditioners can be reduced. In winter, that the angle of incidence is smaller, a larger wavelength region is reflected, where the infrared intensity is very low.

  16. Local charge measurement using off-axis electron holography

    NASA Astrophysics Data System (ADS)

    Beleggia, M.; Gontard, L. C.; Dunin-Borkowski, R. E.

    2016-07-01

    A model-independent approach based on Gauss’ theorem for measuring the local charge in a specimen from an electron-optical phase image recorded using off-axis electron holography was recently proposed. Here, we show that such a charge measurement is reliable when it is applied to determine the total charge enclosed within an object. However, the situation is more complicated for a partial charge measurement when the integration domain encloses only part of the object. We analyze in detail the effects on charge measurement of the mean inner potential of the object, of the presence of induced charges on nearby supports/electrodes and of noise. We perform calculations for spherical particles and highlight the differences when dealing with other object shapes. Our analysis is tested using numerical simulations and applied to the interpretation of an experimental dataset recorded from a sapphire particle.

  17. Synthetic holography in microscopy: opportunities arising from advanced wavefront shaping

    NASA Astrophysics Data System (ADS)

    Jesacher, Alexander; Ritsch-Marte, Monika

    2016-01-01

    The advent of computer-generated or synthetic holography has created a wealth of possibilities for wavefront shaping in optics. We discuss the impact this has had on optical microscopy. Synthetic Holographic Microscopy utilises wavefront shaping by a computer-generated 'hologram' (CGH) to modify light on the illumination or the detection side, or both. This enables modifications of the general sample appearance concerning contrast, resolution and other aspects. Multiplexing CGHs can perform several tasks at once, for instance splitting the image into sub-images corresponding to different depths in the sample, or displaying differently contrasted images of the sample, e.g. bright field, darkfield or (spiral) phase contrast, in different sub-images. We give an overview of the options and discuss the advantages and disadvantages of using programmable holographic elements inside an optical microscope.

  18. On generalized gravitational entropy, squashed cones and holography

    NASA Astrophysics Data System (ADS)

    Bhattacharyya, Arpan; Sharma, Menika; Sinha, Aninda

    2014-01-01

    We consider generalized gravitational entropy in various higher derivative theories of gravity dual to four dimensional CFTs using the recently proposed regularization of squashed cones. We derive the universal terms in the entanglement entropy for spherical and cylindrical surfaces. This is achieved by constructing the Fefferman-Graham expansion for the leading order metrics for the bulk geometry and evaluating the generalized gravitational entropy. We further show that the Wald entropy evaluated in the bulk geometry constructed for the regularized squashed cones leads to the correct universal parts of the entanglement entropy for both spherical and cylindrical entangling surfaces. We comment on the relation with the Iyer-Wald formula for dynamical horizons relating entropy to a Noether charge. Finally we show how to derive the entangling surface equation in Gauss-Bonnet holography.

  19. Two-color holography concept (T-CHI)

    NASA Technical Reports Server (NTRS)

    Vikram, C. S.; Caulfield, H. J.; Workman, G. L.; Trolinger, J. D.; Wood, C. P.; Clark, R. L.; Kathman, A. D.; Ruggiero, R. M.

    1990-01-01

    The Material Processing in the Space Program of NASA-MSFC was active in developing numerous optical techniques for the characterization of fluids in the vicinity of various materials during crystallization and/or solidification. Two-color holographic interferometry demonstrates that temperature and concentration separation in transparent (T-CHI) model systems is possible. The experiments were performed for particular (succinonitrile) systems. Several solutions are possible in Microgravity Sciences and Applications (MSA) experiments on future Shuttle missions. The theory of the T-CHI concept is evaluated. Although particular cases are used for explanations, the concepts developed will be universal. A breadboard system design is also presented for ultimate fabrication and testing of theoretical findings. New developments in holography involving optical fibers and diode lasers are also incorporated.

  20. Holography and the Coleman-Mermin-Wagner theorem

    SciTech Connect

    Anninos, Dionysios; Hartnoll, Sean A.; Iqbal, Nabil

    2010-09-15

    In 2+1 dimensions at finite temperature, spontaneous symmetry breaking of global symmetries is precluded by large thermal fluctuations of the order parameter. The holographic correspondence implies that analogous effects must also occur in 3+1 dimensional theories with gauged symmetries in certain curved spacetimes with horizon. By performing a one loop computation in the background of a holographic superconductor, we show that bulk quantum fluctuations wash out the classical order parameter at sufficiently large distance scales. The low temperature phase is seen to exhibit algebraic long-range order. Beyond the specific example we study, holography suggests that IR singular quantum fluctuations of the fields and geometry will play an interesting role for many 3+1 dimensional asymptotically anti-de Sitter spacetimes with planar horizon.

  1. Indefinite Plasmonic Beam Engineering by In-plane Holography.

    PubMed

    Chen, J; Li, L; Li, T; Zhu, S N

    2016-01-01

    Recent advances in controlling the optical phase at the sub-wavelength scale by meta-structures offer unprecedented possibilities in the beam engineering, holograms, and even invisible cloaks. In despite of developments of plasmonic beam engineering for definite beams, here, we proposed a new holographic strategy by in-plane diffraction process to access indefinite plasmonic beams, where a counterintuitive oscillating beam was achieved at a free metal surface that is against the common recognition of light traveling. Beyond the conventional hologram, our approach emphasizes on the phase correlation on the target, and casts an in-depth insight into the beam formation as a kind of long depth-of-field object. Moreover, in contrast to previous plasmonic holography with space light as references, our approach is totally fulfilled in a planar dimension that offers a thoroughly compact manipulation of the plasmonic near-field and suggests new possibilities in nanophotonic designs. PMID:27357133

  2. Time-average TV holography for vibration fringe analysis

    SciTech Connect

    Kumar, Upputuri Paul; Kalyani, Yanam; Mohan, Nandigana Krishna; Kothiyal, Mahendra Prasad

    2009-06-01

    Time-average TV holography is widely used method for vibration measurement. The method generates speckle correlation time-averaged J0 fringes that can be used for full-field qualitative visualization of mode shapes at resonant frequencies of an object under harmonic excitation. In order to map the amplitudes of vibration, quantitative evaluation of the time-averaged fringe pattern is desired. A quantitative evaluation procedure based on the phase-shifting technique used in two beam interferometry has also been adopted for this application with some modification. The existing procedure requires a large number of frames to be recorded for implementation. We propose a procedure that will reduce the number of frames required for the analysis. The TV holographic system used and the experimental results obtained with it on an edge-clamped, sinusoidally excited square aluminium plate sample are discussed.

  3. Indefinite Plasmonic Beam Engineering by In-plane Holography

    PubMed Central

    Chen, J.; Li, L.; Li, T.; Zhu, S. N.

    2016-01-01

    Recent advances in controlling the optical phase at the sub-wavelength scale by meta-structures offer unprecedented possibilities in the beam engineering, holograms, and even invisible cloaks. In despite of developments of plasmonic beam engineering for definite beams, here, we proposed a new holographic strategy by in-plane diffraction process to access indefinite plasmonic beams, where a counterintuitive oscillating beam was achieved at a free metal surface that is against the common recognition of light traveling. Beyond the conventional hologram, our approach emphasizes on the phase correlation on the target, and casts an in-depth insight into the beam formation as a kind of long depth-of-field object. Moreover, in contrast to previous plasmonic holography with space light as references, our approach is totally fulfilled in a planar dimension that offers a thoroughly compact manipulation of the plasmonic near-field and suggests new possibilities in nanophotonic designs. PMID:27357133

  4. Ultrasonic Imaging of Subsurface Objects Using Photorefractive Dynamic Holography

    SciTech Connect

    Deason, Vance Albert; Telschow, Kenneth Louis; Watson, Scott Marshall

    2001-07-01

    The INEEL has developed a photorefractive ultrasonic imaging technology that records both phase and amplitude of ultrasonic waves on the surface of solids. Phase locked dynamic holography provides full field images of these waves scattered from subsurface defects in solids, and these data are compared with theoretical predictions. Laser light reflected by a vibrating surface is imaged into a photorefractive material where it is mixed in a heterodyne technique with a reference wave. This demodulates the data and provides an image of the ultrasonic waves in either 2 wave or 4 wave mixing mode. These data images are recorded at video frame rates and show phase locked traveling or resonant acoustic waves. This technique can be used over a broad range of ultrasonic frequencies. Acoustic frequencies from 2 kHz to 10 MHz have been imaged, and a point measuring (non-imaging) version of the system has measured picometer amplitudes at 1 GHz.

  5. Ultrasonic imaging of subsurface objects using photorefractive dynamic holography

    NASA Astrophysics Data System (ADS)

    Deason, Vance A.; Telschow, Kenneth L.; Watson, Scott M.

    2001-11-01

    The INEEL has developed a photorefractive ultrasonic imaging technology that records both phase and amplitude of ultrasonic waves on the surface of solids. Phase locked dynamic holography provides full field images of these waves scattered from subsurface defects in solids, and these data are compared with theoretical predictions. Laser light reflected by a vibrating surface is imaged into a photorefractive material where it is mixed in a heterodyne technique with a reference wave. This demodulates the data and provides an image of the ultrasonic waves in either 2 wave or 4 wave mixing mode. These data images are recorded at video frame rates and show phase locked traveling or resonant acoustic waves. This technique can be used over a broad range of ultrasonic frequencies. Acoustic frequencies from 2 kHz to 10 MHz have been imaged, and a point measuring (non-imaging) version of the system has measured picometer amplitudes at 1 Ghz.

  6. SHIVA: Spaceflight Holography Investigation in a Virtual Apparatus

    NASA Technical Reports Server (NTRS)

    Trolinger, James D.; Rangel, Roger; Coimbra, Carlos; Lal, Ravindra B.; Witherow, William; Rogers, Jan

    2000-01-01

    SHIVA, a NASA spaceflight program, will exploit a unique, holography-based, diagnostics tool to test and apply a new, more general analytical solution to a fundamental equation of motion. The equation describes particle dynamics in fluids in a microgravity environment, and our solution represents the first analytical solution of it. When gravity is removed the equation becomes much more complex and had been solved previously only by numerical means. After our analytical solution has been validated it will be used as a tool for making additional measurements of the gravity environment. Our experiment will be optimized for testing the model, measuring g, g-jitter, and other microgravity phenomena. We will also collect data for which no current theory exists, This paper describes ground experiments and analysis that have been conducted by the investigator team to support the flight experiment.

  7. Triboelectricity evaluation of single toner particle by electron holography

    SciTech Connect

    Okada, H.; Shindo, D.; Kim, J. J.; Murakami, Y.; Kawase, H.

    2007-09-01

    Understanding electrification is particularly important in materials science since the use of charged particles, e.g., the electrophotographic printer with toner particles, is one of the most successful applications of electrification. However, the charge generation mechanism still remains unclear due to the lack of an appropriate method for evaluating individual fine particles. In this study, we describe an approach for determining the charge of a single toner particle that uses electron holography in combination with a shielding technique. Two long-standing problems in holographic studies--namely, perturbation of the reference electron wave and unwanted charging by illumination--have been overcome by introducing two types of shields in a microscope. Using this method, the amount of charge on a single toner particle was determined, and the surface charge distribution was found to be inhomogeneous. Furthermore, an in situ observation of triboelectricity was conducted inside the microscope.

  8. Phase measurement error in summation of electron holography series.

    PubMed

    McLeod, Robert A; Bergen, Michael; Malac, Marek

    2014-06-01

    Off-axis electron holography is a method for the transmission electron microscope (TEM) that measures the electric and magnetic properties of a specimen. The electrostatic and magnetic potentials modulate the electron wavefront phase. The error in measurement of the phase therefore determines the smallest observable changes in electric and magnetic properties. Here we explore the summation of a hologram series to reduce the phase error and thereby improve the sensitivity of electron holography. Summation of hologram series requires independent registration and correction of image drift and phase wavefront drift, the consequences of which are discussed. Optimization of the electro-optical configuration of the TEM for the double biprism configuration is examined. An analytical model of image and phase drift, composed of a combination of linear drift and Brownian random-walk, is derived and experimentally verified. The accuracy of image registration via cross-correlation and phase registration is characterized by simulated hologram series. The model of series summation errors allows the optimization of phase error as a function of exposure time and fringe carrier frequency for a target spatial resolution. An experimental example of hologram series summation is provided on WS2 fullerenes. A metric is provided to measure the object phase error from experimental results and compared to analytical predictions. The ultimate experimental object root-mean-square phase error is 0.006 rad (2π/1050) at a spatial resolution less than 0.615 nm and a total exposure time of 900 s. The ultimate phase error in vacuum adjacent to the specimen is 0.0037 rad (2π/1700). The analytical prediction of phase error differs with the experimental metrics by +7% inside the object and -5% in the vacuum, indicating that the model can provide reliable quantitative predictions.

  9. Innovative re-creation of realities in a holographic digital form

    NASA Astrophysics Data System (ADS)

    Wang, Shuo; Hebblewhite, Richard; Osanlou, Ardieshir; Excell, Peter; Di Gennaro, Sonia; Shi, Lishen

    2014-02-01

    Only nature can create, whereas humans can only re-create. This article is an exploration of synergies between art and science in digital holography in relation to art practice and the making of holograms as art works. This is achieved through involvement in the re-creation of a real object (a telescope) as a case study. A digital three-dimensional model suitable for holographic hard copy re-creation is produced. To explore special and immersive environment, real geographical landscape background from Google Earth is added to the model. After a brief introduction to visual art within the context of two and three-dimensional imaging in the form photography and holography, the whole process of producing the three-dimensional model and the environment in which it should be presented, ready for holographic printing is explained.

  10. Development of a novel spectrometer for tongue coating analyzer based on volume holography transmissive grating

    NASA Astrophysics Data System (ADS)

    Ren, Zhong; Liu, Guodong; Dai, Longmin; Huang, Zhen; Zeng, Lvming

    2010-11-01

    Tongue diagnosis (TD) is an important diagnostic methods in the traditional Chinese medicine (TCM). According to the viewpoint of TCM, the changes of the tongue coating (TC) can reflect the pathological state of the patient. And the nature or severity of diseasec can be determined by observing the TC. Over the years, TD is mostly depended on the subjective experience of the Chinese physician. And the diagnostic results will be impacted by.some factors, e.g. the different light sources or environmental brightness. Recently years, the method of digital image processing has been used into the TD. But its application is limited by the complicated algorithm, time-consuming and big error, etc. Therefore, a novel tongue coating analyzer(TCA) is designed in this paper. Meanwhile, a novel spectrometer for TCA based on the volume holography transmissive (VHT) grating is developed. In this spectrometer, since the VHT grating doesn't produce the stray-light due to the absence of the grooves of classical surface-embossed gratings, the VHT grating is used as the diffraction grating instead of the classical plane or concave grating. Experimental results show that the performances of the spectrometer for TCA have been improved by using the VHT grating, optimizing the light-path structure and software algorithm, etc. Compared with the others, this spectrometer for TCA has many advantages, such as, less diffraction, wider spectrum range, higher efficiency and resolution, etc. The spectrum range of the spectrometer for TCA can reach 300-1000nm, its resolution can reach 1nm and the optical density is larger than 3.

  11. Improvement of quality of optical reconstruction of digital Fourier holograms displayed on phase-only SLM by its digital preprocessing

    NASA Astrophysics Data System (ADS)

    Cheremkhin, Pavel A.; Evtikhiev, Nikolay N.; Krasnov, Vitaly V.; Porshneva, Liudmila A.; Rodin, Vladislav G.; Starikov, Sergey N.

    2014-10-01

    Digital holography is popular tool for research and practical applications in various fields of science and technology. Most widespread method of optical reconstruction implements digital hologram display on spatial light modulators (SLM). Optical reconstruction of digital holograms is used for remote display of static and dynamic 2D and 3D scenes, in optical information processing, metrology, interferometry, microscopy, etc. Holograms recorded with digital cameras are amplitude type. Therefore quality of its optical reconstruction with phase SLM is worse compared to amplitude SLM. However application of phase SLM can provide higher diffraction efficiency. To improve quality of optical reconstruction with phase SLM, method of SLM phase modulation depth reduction at digital hologram display is proposed. To our knowledge, this method was applied only in analog holography. Also two other methods of quality improvement are considered: hologram to kinoform conversion and holograms multiplexing. Numerical experiments on modelling of digital Fourier holograms recording and their optical reconstruction by phase SLM were performed. Method of SLM phase modulation depth reduction at digital holograms display was proposed and tested. SLM phase modulation depth ranged from 0 to 2π. Quantity of hologram phase levels equal to 256 corresponds to 2π phase modulation depth. To keep SLM settings while changing phase modulation depth hologram phase distribution was renormalized instead. Dependencies of reconstruction quality on hologram phase modulation depth were obtained. Best quality is achieved at 0.27π÷0.31π phase modulation depth. To reduce speckle noise, hologram multiplexing can be applied. Modeling of multiplex holograms optical reconstruction was conducted. Speckle noise reduction was achieved. For improvement of digital hologram optical reconstruction quality and diffraction efficiency hologram to kinoform conversion can be used. Firstly numerically reconstructed image

  12. Digital Libraries.

    ERIC Educational Resources Information Center

    Fox, Edward A.; Urs, Shalini R.

    2002-01-01

    Provides an overview of digital libraries research, practice, and literature. Highlights include new technologies; redefining roles; historical background; trends; creating digital content, including conversion; metadata; organizing digital resources; services; access; information retrieval; searching; natural language processing; visualization;…

  13. Influence of surface position along the working range of conoscopic holography sensors on dimensional verification of AISI 316 wire EDM machined surfaces.

    PubMed

    Fernández, Pedro; Blanco, David; Rico, Carlos; Valiño, Gonzalo; Mateos, Sabino

    2014-03-06

    Conoscopic holography (CH) is a non-contact interferometric technique used for surface digitization which presents several advantages over other optical techniques such as laser triangulation. Among others, the ability for the reconstruction of high-sloped surfaces stands out, and so does its lower dependence on surface optical properties. Nevertheless, similarly to other optical systems, adjustment of CH sensors requires an adequate selection of configuration parameters for ensuring a high quality surface digitizing. This should be done on a surface located as close as possible to the stand-off distance by tuning frequency (F) and power (P) until the quality indicators Signal-to-Noise Ratio (SNR) and signal envelope (Total) meet proper values. However, not all the points of an actual surface are located at the stand-off distance, but they could be located throughout the whole working range (WR). Thus, the quality of a digitized surface may not be uniform. The present work analyses how the quality of a reconstructed surface is affected by its relative position within the WR under different combinations of the parameters F and P. Experiments have been conducted on AISI 316 wire EDM machined flat surfaces. The number of high-quality points digitized as well as distance measurements between different surfaces throughout the WR allowed for comparing the metrological behaviour of the CH sensor with respect to a touch probe (TP) on a CMM.

  14. Influence of Surface Position along the Working Range of Conoscopic Holography Sensors on Dimensional Verification of AISI 316 Wire EDM Machined Surfaces

    PubMed Central

    Fernández, Pedro; Blanco, David; Rico, Carlos; Valiño, Gonzalo; Mateos, Sabino

    2014-01-01

    Conoscopic holography (CH) is a non-contact interferometric technique used for surface digitization which presents several advantages over other optical techniques such as laser triangulation. Among others, the ability for the reconstruction of high-sloped surfaces stands out, and so does its lower dependence on surface optical properties. Nevertheless, similarly to other optical systems, adjustment of CH sensors requires an adequate selection of configuration parameters for ensuring a high quality surface digitizing. This should be done on a surface located as close as possible to the stand-off distance by tuning frequency (F) and power (P) until the quality indicators Signal-to-Noise Ratio (SNR) and signal envelope (Total) meet proper values. However, not all the points of an actual surface are located at the stand-off distance, but they could be located throughout the whole working range (WR). Thus, the quality of a digitized surface may not be uniform. The present work analyses how the quality of a reconstructed surface is affected by its relative position within the WR under different combinations of the parameters F and P. Experiments have been conducted on AISI 316 wire EDM machined flat surfaces. The number of high-quality points digitized as well as distance measurements between different surfaces throughout the WR allowed for comparing the metrological behaviour of the CH sensor with respect to a touch probe (TP) on a CMM. PMID:24662452

  15. Study of 3D remote sensing system based on optical scanning holography

    NASA Astrophysics Data System (ADS)

    Zhao, Shihu; Yan, Lei

    2009-06-01

    High-precision and real-time remote sensing imaging system is an important part of remote sensing development. Holography is a method of wave front record and recovery which was presented by Dennis Gabor. As a new kind of holography techniques, Optical scanning holography (OSH) and remote sensing imaging are intended to be combined together and applied in acquisition and interference measurement of remote sensing. The key principles and applicability of OSH are studied and the mathematic relation between Fresnel Zone Plate number, numerical aperture and object distance was deduced, which are proved to be feasible for OSH to apply in large scale remote sensing. At last, a new three-dimensional reflected OSH remote sensing imaging system is designed with the combination of scanning technique to record hologram patterns of large scale remote sensing scenes. This scheme is helpful for expanding OSH technique to remote sensing in future.

  16. Photorefractive and computational holography in the experimental generation of Airy beams

    NASA Astrophysics Data System (ADS)

    Suarez, Rafael A. B.; Vieira, Tarcio A.; Yepes, Indira S. V.; Gesualdi, Marcos R. R.

    2016-05-01

    In this paper, we present the experimental generation of Airy beams via computational and photorefractive holography. Experimental generation of Airy beams using conventional optical components presents several difficulties and a practically infeasible. Thus, the optical generation of Airy beams has been made from the optical reconstruction of a computer generated hologram implemented by a spatial light modulator. In the photorefractive holography technique, being used for the first time to our knowledge, the hologram of an Airy beam is constructed (recorded) and reconstructed (read) optically in a nonlinear photorefractive medium. The Airy beam experimental realization was made by a setup of computational and photorefractive holography using a photorefractive Bi12 TiO20 crystal as holographic recording medium. Airy beams and Airy beam arrays were obtained experimentally in accordance with the predicted theory; with excellent prospects for applications in optical trapping and optical communications systems.

  17. Holography as a principle in quantum gravity?-Some historical and systematic observations

    NASA Astrophysics Data System (ADS)

    Sieroka, Norman; Mielke, Eckehard W.

    2014-05-01

    Holography is a fruitful concept in modern physics. However, there is no generally accepted definition of the term, and its significance, especially as a guiding principle in quantum gravity, is rather uncertain. The present paper critically evaluates variants of the holographic principle from two perspectives: (i) their relevance in contemporary approaches to quantum gravity and in closely related areas; (ii) their historical forerunners in the early twentieth century and the role played by past and present concepts of holography in attempts to unify physics. By combining these two perspectives a certain depth of focus is gained which allows us to draw some tentative conclusions about what might be reasonable aspirations and prospects for holography in quantum gravity. By the same token, we will have a brief and critical look at wider philosophical interpretations of the term.

  18. Time-domain optical coherence tomography with digital holographic microscopy

    NASA Astrophysics Data System (ADS)

    Massatsch, Pia; Charrière, Florian; Cuche, Etienne; Marquet, Pierre; Depeursinge, Christian D.

    2005-04-01

    We show that digital holography can be combined easily with optical coherence tomography approach. Varying the reference path length is the means used to acquire a series of holograms at different depths, providing after reconstruction images of slices at different depths in the specimen thanks to the short-coherence length of light source. A metallic object, covered by a 150-µm-thick onion cell, is imaged with high resolution. Applications in ophthalmology are shown: structures of the anterior eye, the cornea, and the iris, are studied on enucleated porcine eyes. Tomographic images of the iris border close to the pupil were obtained 165 µm underneath the eye surface.

  19. Digital Preservation.

    ERIC Educational Resources Information Center

    Yakel, Elizabeth

    2001-01-01

    Reviews research on digital preservation issues, including born-digital and digitally recreated documents. Discusses electronic records research; metadata and other standards; electronic mail; Web-based documents; moving images media; selection of materials for digitization, including primary sources; administrative issues; media stability…

  20. Computational spectral microscopy and compressive millimeter-wave holography

    NASA Astrophysics Data System (ADS)

    Fernandez, Christy Ann

    This dissertation describes three computational sensors. The first sensor is a scanning multi-spectral aperture-coded microscope containing a coded aperture spectrometer that is vertically scanned through a microscope intermediate image plane. The spectrometer aperture-code spatially encodes the object spectral data and nonnegative least squares inversion combined with a series of reconfigured two-dimensional (2D spatial-spectral) scanned measurements enables three-dimensional (3D) (x, y, lambda) object estimation. The second sensor is a coded aperture snapshot spectral imager that employs a compressive optical architecture to record a spectrally filtered projection of a 3D object data cube onto a 2D detector array. Two nonlinear and adapted TV-minimization schemes are presented for 3D (x, y, lambda) object estimation from a 2D compressed snapshot. Both sensors are interfaced to laboratory-grade microscopes and applied to fluorescence microscopy. The third sensor is a millimeter-wave holographic imaging system that is used to study the impact of 2D compressive measurement on 3D (x, y, z) data estimation. Holography is a natural compressive encoder since a 3D parabolic slice of the object band volume is recorded onto a 2D planar surface. An adapted nonlinear TV-minimization algorithm is used for 3D tomographic estimation from a 2D and a sparse 2D hologram composite. This strategy aims to reduce scan time costs associated with millimeter-wave image acquisition using a single pixel receiver.

  1. Interstellar Message Plaques: Application of White-Light Holography

    NASA Astrophysics Data System (ADS)

    Matloff, G. L.

    2002-01-01

    During Spring / Summer 2001, a prototype white-light holographic interstellar-probe message plaque was created under Contract H-29712D of NASA Marshall Spaceflight Center (MSFC), and commercial white-light holograms were tested for space-radiation tolerance at the MSFC Space Environment Facility (SEF) in Huntsville, AL, USA. Artist C Bangs' message plaque was created at the Center for Holographic Arts in Long Island City, NY. The 57.5 X 47.5 cm rainbow hologram was delivered to MSFC after framing by Simon Liu Inc., Brooklyn, NY, USA. The prototype message plaque, which is in the collection of the MSFC Space Transportation Directorate, has six multiplexed 2-D and 3-D images representing humans, the hypothetical interstellar spacecraft, and our position in the galaxy. Consultation with John Caulfield of Fisk University, an expert in holography, revealed that micron-thick holograms not much larger than a sheet of paper could contain hundreds of thousands of images, which opens the me ssage-plaque field considerably so that work of many artists could be included. Tests of commercial holograms at up to 100 MRad of simulated solar-wind radiation were performed at MSFC / SEF. Image-quality deterioriation was monitored using the image-color- histogram of the (trademarked) Adobe Photoshop software package. No significant deterioration occurred, which is in agreement with the literature. Holographic solar sails may be a propulsive application of this technology.

  2. Off-axis electron holography of ferromagnetic multilayer nanowires

    SciTech Connect

    Akhtari-Zavareh, Azadeh; Kavanagh, K. L.; Carignan, L. P.; Yelon, A.; Ménard, D.; Kasama, T.; Herring, R.; Dunin-Borkowski, R. E.; McCartney, M. R.

    2014-07-14

    We have used electron holography to investigate the local magnetic behavior of isolated ferromagnetic nanowires (NWs) in their remanent states. The NWs consisted of periodic magnetic layers of soft, high-saturation magnetization CoFeB alloys, and non-magnetic layers of Cu. All NWs were fabricated by pulsed-potential electrodeposition in nanoporous alumina membranes. The NW composition and layer thicknesses were measured using scanning transmission electron microscopy and energy dispersive spectroscopy. The magnetization of individual NWs depended upon the thicknesses of the layers and the direction of an external magnetic field, which had been applied in situ. When the CoFeB was thicker than the diameter (50 nm), magnetization was axial for all external field directions, while thinner layers could be randomized via a perpendicular field. In some cases, magnetization inside the wire was detected at an angle with respect to the axis of the wires. In thinner Cu/CoFeB (<10 nm each) multilayer, magnetic field vortices were detected, associated with opposing magnetization in neighbouring layers. The measured crystallinity, compositions, and layer thicknesses of individual NWs were found to be significantly different from those predicted from calibration growths based on uniform composition NWs. In particular, a significant fraction of Cu (up to 50 at. %) was present in the CoFeB layers such that the measured magnetic induction was lower than expected. These results will be used to better understand previously measured effective anisotropy fields of similar NW arrays.

  3. Holography and quantum states in elliptic de Sitter space

    NASA Astrophysics Data System (ADS)

    Halpern, Illan F.; Neiman, Yasha

    2015-12-01

    We outline a program for interpreting the higher-spin dS/CFT model in terms of physics in the causal patch of a dS observer. The proposal is formulated in "elliptic" de Sitter space d{S}_4/{Z}_2 , obtained by identifying antipodal points in dS 4. We discuss recent evidence that the higher-spin model is especially well-suited for this, since the antipodal symmetry of bulk solutions has a simple encoding on the boundary. For context, we test some other (free and interacting) theories for the same property. Next, we analyze the notion of quantum field states in the non-time-orientable d{S}_4/{Z}_2 . We compare the physics seen by different observers, with the outcome depending on whether they share an arrow of time. Finally, we implement the marriage between higher-spin holography and observers in d{S}_4/{Z}_2 , in the limit of free bulk fields. We succeed in deriving an observer's operator algebra and Hamiltonian from the CFT, but not her S-matrix. We speculate on the extension of this to interacting higher-spin theory.

  4. Low Complexity Compression and Speed Enhancement for Optical Scanning Holography

    PubMed Central

    Tsang, P. W. M.; Poon, T.-C.; Liu, J.-P.; Kim, T.; Kim, Y. S.

    2016-01-01

    In this paper we report a low complexity compression method that is suitable for compact optical scanning holography (OSH) systems with different optical settings. Our proposed method can be divided into 2 major parts. First, an automatic decision maker is applied to select the rows of holographic pixels to be scanned. This process enhances the speed of acquiring a hologram, and also lowers the data rate. Second, each row of down-sampled pixels is converted into a one-bit representation with delta modulation (DM). Existing DM-based hologram compression techniques suffers from the disadvantage that a core parameter, commonly known as the step size, has to be determined in advance. However, the correct value of the step size for compressing each row of hologram is dependent on the dynamic range of the pixels, which could deviate significantly with the object scene, as well as OSH systems with different opical settings. We have overcome this problem by incorporating a dynamic step-size adjustment scheme. The proposed method is applied in the compression of holograms that are acquired with 2 different OSH systems, demonstrating a compression ratio of over two orders of magnitude, while preserving favorable fidelity on the reconstructed images. PMID:27708410

  5. ELECTRON HOLOGRAPHY OF ELECTROMAGNETIC FIELDS - RECENT THEORETICAL ADVANCES.

    SciTech Connect

    BELEGGIA,M.; POZZI, G.; TONOMURA, A.

    2007-01-01

    It has been shown in this work that the Fourier space approach can be fruitfully applied to the calculation of the fields and the associated electron optical phase shift of several magnetic and electrostatic structures, like superconducting vortices in conventional and high-T{sub c} superconductors, reverse biased p-n junctions, magnetic domains and nanoparticles. In all these cases, this novel approach has led to unexpected but extremely interesting results, very often expressed in analytical form, which allow the quantitative and reliable interpretation of the experimental data collected by means of electron holography or of more conventional Lorentz microscopy techniques. Moreover, it is worth recalling that whenever long-range electromagnetic fields are involved, a physical model of the object under investigation is necessary in order to take into account correctly the perturbation of the reference wave induced by the tail of the field protruding into the vacuum. For these reasons, we believe that the Fourier space approach for phase computations we have introduced and discussed in this chapter will represent an invaluable tool for the investigation of electromagnetic fields at the meso- and nano-scale.

  6. Low Complexity Compression and Speed Enhancement for Optical Scanning Holography

    NASA Astrophysics Data System (ADS)

    Tsang, P. W. M.; Poon, T.-C.; Liu, J.-P.; Kim, T.; Kim, Y. S.

    2016-10-01

    In this paper we report a low complexity compression method that is suitable for compact optical scanning holography (OSH) systems with different optical settings. Our proposed method can be divided into 2 major parts. First, an automatic decision maker is applied to select the rows of holographic pixels to be scanned. This process enhances the speed of acquiring a hologram, and also lowers the data rate. Second, each row of down-sampled pixels is converted into a one-bit representation with delta modulation (DM). Existing DM-based hologram compression techniques suffers from the disadvantage that a core parameter, commonly known as the step size, has to be determined in advance. However, the correct value of the step size for compressing each row of hologram is dependent on the dynamic range of the pixels, which could deviate significantly with the object scene, as well as OSH systems with different opical settings. We have overcome this problem by incorporating a dynamic step-size adjustment scheme. The proposed method is applied in the compression of holograms that are acquired with 2 different OSH systems, demonstrating a compression ratio of over two orders of magnitude, while preserving favorable fidelity on the reconstructed images.

  7. Light-Front Holography and Hadronization at the Amplitude Level

    SciTech Connect

    Brodsky, Stanley J.; Teramond, Guy F. de; Shrock, Robert

    2008-10-13

    The correspondence between theories in anti-de Sitter space and conformal field theories in physical space-time leads to an analytic, semiclassical model for strongly-coupled QCD which has scale invariance at short distances and color confinement at large distances. Light-front holography is a remarkable feature of AdS/CFT: it allows hadronic amplitudes in the AdS fifth dimension to be mapped to frame-independent light-front wavefunctions of hadrons in physical space-time, thus providing a relativistic description of hadrons at the amplitude level. Some novel features of QCD are discussed, including the consequences of confinement for quark and gluon condensates and the behavior of the QCD coupling in the infrared. We suggest that the spatial support of QCD condensates is restricted to the interior of hadrons, since they arise due to the interactions of confined quarks and gluons. Chiral symmetry is thus broken in a limited domain of size 1/m{sub {pi}}, in analogy to the limited physical extent of superconductor phases. A new method for computing the hadronization of quark and gluon jets at the amplitude level, an event amplitude generator, is outlined.

  8. Important Cultural Properties Recorded By Means Of Holography

    NASA Astrophysics Data System (ADS)

    Katsuma, Hidetoshi; Sato, Koki

    1987-01-01

    There are many important properties in Japan. A teacup for tea ceremony in ancient time is shown in Fig.l. This one is easily recorded on Fresnel and Lippman holograms. Especially,color Lippman holography1)12) is good example. But wooden startue of Buda in Fig.2 is not removed to record. Reflection holographic stereograms(R.H.S)3)are used for one. This R.H.S is very interesting because of this ability to render a wide renge of input formates,such as photographic,video,computer-graphic,and movie images.Our experiments are made this R.H.S into color one.Red image is necessary to use He-Ne laser, blue and green is Ar laser.The flat type of R.H.S4) is useful for this experiment.To repair these properties will be remarkable progressive advanced by making use of computer-graphics and this R.H.S.

  9. Light-Front Holography and Novel Effects in QCD

    SciTech Connect

    Brodsky, Stanley J.; de Teramond, Guy F.

    2008-12-18

    The correspondence between theories in anti-de Sitter space and conformal field theories in physical space-time leads to an analytic, semiclassical model for strongly-coupled QCD. Light-front holography allows hadronic amplitudes in the AdS fifth dimension to be mapped to frame-independent light-front wavefunctions of hadrons in physical space-time, thus providing a relativistic description of hadrons at the amplitude level. We identify the AdS coordinate z with an invariant light-front coordinate {zeta} which separates the dynamics of quark and gluon binding from the kinematics of constituent spin and internal orbital angular momentum. The result is a single-variable light-front Schroedinger equation for QCD which determines the eigenspectrum and the light-front wavefunctions of hadrons for general spin and orbital angular momentum. The mapping of electromagnetic and gravitational form factors in AdS space to their corresponding expressions in light-front theory confirms this correspondence. Some novel features of QCD are discussed, including the consequences of confinement for quark and gluon condensates and the behavior of the QCD coupling in the infrared. The distinction between static structure functions such as the probability distributions computed from the square of the light-front wavefunctions versus dynamical structure functions which include the effects of rescattering is emphasized. A new method for computing the hadronization of quark and gluon jets at the amplitude level, an event amplitude generator, is outlined.

  10. Lock-in camera based heterodyne holography for ultrasound-modulated optical tomography inside dynamic scattering media

    NASA Astrophysics Data System (ADS)

    Liu, Yan; Shen, Yuecheng; Ma, Cheng; Shi, Junhui; Wang, Lihong V.

    2016-06-01

    Ultrasound-modulated optical tomography (UOT) images optical contrast deep inside scattering media. Heterodyne holography based UOT is a promising technique that uses a camera for parallel speckle detection. In previous works, the speed of data acquisition was limited by the low frame rates of conventional cameras. In addition, when the signal-to-background ratio was low, these cameras wasted most of their bits representing an informationless background, resulting in extremely low efficiencies in the use of bits. Here, using a lock-in camera, we increase the bit efficiency and reduce the data transfer load by digitizing only the signal after rejecting the background. Moreover, compared with the conventional four-frame based amplitude measurement method, our single-frame method is more immune to speckle decorrelation. Using lock-in camera based UOT with an integration time of 286 μs, we imaged an absorptive object buried inside a dynamic scattering medium exhibiting a speckle correlation time ( τ c ) as short as 26 μs. Since our method can tolerate speckle decorrelation faster than that found in living biological tissue ( τ c ˜ 100-1000 μs), it is promising for in vivo deep tissue non-invasive imaging.

  11. Split-illumination electron holography for improved evaluation of electrostatic potential associated with electrophotography

    SciTech Connect

    Tanigaki, Toshiaki Aizawa, Shinji; Soon Park, Hyun; Sato, Kuniaki; Akase, Zentaro; Matsuda, Tsuyoshi; Murakami, Yasukazu; Shindo, Daisuke; Kawase, Hiromitsu

    2014-03-31

    Precise evaluation of the electrostatic potential distributions of and around samples with multiple charges using electron holography has long been a problem due to unknown perturbation of the reference wave. Here, we report the first practical application of split-illumination electron holography (SIEH) to tackle this problem. This method enables the use of a non-perturbed reference wave distant from the sample. SIEH revealed the electrostatic potential distributions at interfaces of the charged particles used for development in electrophotography and should lead to dramatic improvements in electrophotography.

  12. Fabrication of submicron metallic grids with interference and phase-mask holography

    SciTech Connect

    Park, Joong-Mok; Kim, Tae-Geun; Constant, Kristen; Ho, Kai-Ming

    2011-01-25

    Complex, submicron Cu metallic mesh nanostructures are made by electrochemical deposition using polymer templates made from photoresist. The polymer templates are fabricated with photoresist using two-beam interference holography and phase mask holography with three diffracted beams. Freestanding metallic mesh structures are made in two separate electrodepositions with perpendicular photoresist grating templates. Cu mesh square nanostructures having large (52.6%) open areas are also made by single electrodeposition with a photoresist template made with a phase mask. These structures have potential as electrodes in photonic devices.

  13. 2D wave-front shaping in optical superlattices using nonlinear volume holography.

    PubMed

    Yang, Bo; Hong, Xu-Hao; Lu, Rong-Er; Yue, Yang-Yang; Zhang, Chao; Qin, Yi-Qiang; Zhu, Yong-Yuan

    2016-07-01

    Nonlinear volume holography is employed to realize arbitrary wave-front shaping during nonlinear processes with properly designed 2D optical superlattices. The concept of a nonlinear polarization wave in nonlinear volume holography is investigated. The holographic imaging of irregular patterns was performed using 2D LiTaO3 crystals with fundamental wave propagating along the spontaneous polarization direction, and the results agree well with the theoretical predictions. This Letter not only extends the application area of optical superlattices, but also offers an efficient method for wave-front shaping technology.

  14. Subcycle interference dynamics of time-resolved photoelectron holography with midinfrared laser pulses

    SciTech Connect

    Bian Xuebin; Yuan, Kai-Jun; Bandrauk, Andre D.; Huismans, Y.; Smirnova, O.; Vrakking, M. J. J.

    2011-10-15

    Time-resolved photoelectron holography from atoms using midinfrared laser pulses is investigated by solving the corresponding time-dependent Schroedinger equation (TDSE) and a classical model, respectively. The numerical simulation of the photoelectron angular distribution of Xe irradiated with a low-frequency free-electron laser source agrees well with the experimental results. Different types of subcycle interferometric structures are predicted by the classical model. Furthermore with the TDSE model it is demonstrated that the holographic pattern is sensitive to the shape of the atomic orbitals. This is a step toward imaging by means of photoelectron holography.

  15. Digital holographic microscopy for the evaluation of human sperm structure.

    PubMed

    Coppola, G; Di Caprio, G; Wilding, M; Ferraro, P; Esposito, G; Di Matteo, L; Dale, R; Coppola, G; Dale, B

    2014-11-01

    The morphology of the sperm head has often been correlated with the outcome of in vitro fertilization, and has been shown to be the sole parameter in semen of value in predicting the success of intracytoplasmic sperm injection and intracytoplasmic morphologically selected sperm injection. In this paper, we have studied whether digital holographic microscopy (DHM) may be useful to obtain quantitative data on human sperm head structure and compared this technique with high-power digitally enhanced Nomarski optics. The main advantage of digital holography is that high-resolution three-dimensional quantitative sample imaging may be automatically produced by numerical refocusing of a two-dimensional image at different object planes without any mechanical scanning. We show that DHM generates useful information on the dimensions and structure of human sperm, not revealed by conventional phase-contrast microscopy, in particular the volume of vacuoles, and suggest its use as an additional prognostic tool in assisted reproduction technology.

  16. Deterministic phase encoding encryption in arbitrary phase-step digital holography

    NASA Astrophysics Data System (ADS)

    Chang, Chi-Ching; Hsieh, Wang Ta; Kuo, Ming Kuei

    2010-05-01

    A deterministic phase-encoded encryption system, which adopts a lenticular lens array (LLA) sheet as a phase modulator (key), based on arbitrary two-step phase-shift interferometry (PSI), with an unknown phase step, is presented. The principle of encryption and decryption which is using a LLA in arbitrary unknown two-step PSI is given. With the aid of key holograms (right key), it can be theoretically shown that only the reconstructed object wavefront term will be left in the image plane, and all the accompany undesired terms be eliminated. Thus the hidden information of object wavefront in this encryption system can be numerically and successfully decrypted using arbitrary unknown two-step PSI with right key. For comparisons, computer simulations are carried out to verify the principle of encryption and decryption without key, with wrong key and with right key, respectively.

  17. Imaging the cellular response to transient shear stress using time-resolved digital holography

    NASA Astrophysics Data System (ADS)

    Arita, Yoshihiko; Antkowiak, Maciej; Gunn-Moore, Frank; Dholakia, Kishan

    2014-02-01

    Shear stress has been recognized as one of the biophysical methods by which to permeabilize plasma membranes of cells. In particular, high pressure transient hydrodynamic flows created by laser-induced cavitation have been shown to lead to the uptake of fluorophores and plasmid DNA. While the mechanism and dynamics of cavitation have been extensively studied using a variety of time-resolved imaging techniques, the cellular response to the cavitation bubble and cavitation induced transient hydrodynamic flows has never been shown in detail. We use time-resolved quantitative phase microscopy to study cellular response to laser-induced cavitation bubbles. Laser-induced breakdown of an optically trapped polystyrene nanoparticle (500nm in diameter) irradiated with a single nanosecond laser pulse at 532nm creates transient shear stress to surrounding cells without causing cell lysis. A bi-directional transient displacement of cytoplasm is observed during expansion and collapse of the cavitation bubble. In some cases, cell deformation is only observable at the microsecond time scale without any permanent change in cell shape or optical thickness. On a time scale of seconds, the cellular response to shear stress and cytoplasm deformation typically leads to retraction of the cellular edge most exposed to the flow, rounding of the cell body and, in some cases, loss of cellular dry mass. These results give a new insight into the cellular response to laser-induced shear stress and related plasma membrane permeabilization. This study also demonstrates that laser-induced breakdown of an optically trapped nanoparticle offers localized cavitation (70 μm in diameter), which interacts with a single cell.

  18. Multiplane imaging and depth-of-focus extending in digital holography by a single-shot digital hologram

    NASA Astrophysics Data System (ADS)

    Pan, Weiqing

    2013-01-01

    Limited depth of field is the main drawback of conventional microscopy that prevents observation of thick semi-transparent objects with all their features in focus, only a portion of the imaged volume along the optical axis is in good focus at once. The paper presents a novel reconstruction algorithm to image multiple planes at different depths simultaneously and realize extended focused imaging. A shift parameter that accounts for the coordinate system's transverse displacement of the image plane at different depths is introduced in the diffraction integral kernel. Combination of the diffraction integral kernel with different shift values and reconstruction depths yields multiplane imaging resolution in a single reconstruction. Moreover an extended depth-of-focus method is also presented through modifying the proposed multiplane imaging algorithm. Description of the method and experimental results are reported.

  19. Feasibility study of complex wavefield retrieval in off-axis acoustic holography employing an acousto-optic sensor

    PubMed Central

    Rodríguez, Guillermo López; Weber, Joshua; Sandhu, Jaswinder Singh; Anastasio, Mark A.

    2011-01-01

    We propose and experimentally demonstrate a new method for complex-valued wavefield retrieval in off-axis acoustic holography. The method involves use of an intensity-sensitive acousto-optic (AO) sensor, optimized for use at 3.3 MHz, to record the acoustic hologram and a computational method for reconstruction of the object wavefield. The proposed method may circumvent limitations of conventional implementations of acoustic holography and may facilitate the development of acoustic-holography-based biomedical imaging methods. PMID:21669451

  20. Digital holographic diagnostics of near-injector region

    NASA Astrophysics Data System (ADS)

    Lee, Jaiho

    Study of primary breakup of liquid jets is important because it is motivated by the application to gas turbine fuel injectors, diesel fuel injectors, industrial cleaning and washing machine, medical spray, and inkjet printers, among others. When it comes to good injectors, a liquid jet has to be disintegrated into a fine spray near injector region during primary breakup. However the dense spray region near the injectors is optically obscure for Phase Doppler Interferometer like Phase Doppler Particle Analyzers (PDPA). Holography can provide three dimensional image of the dense spray and eliminate the problem of the small depth of focus associated with shadowgraphs. Traditional film-based holographic technique has long been used for three dimensional measurements in particle fields, but it is time consuming, expensive, chemically hazardous. With the development of the CCD sensor, holograms were recorded and reconstructed digitally. Digital microscopic holography (DMH) is similar to digital inline holography (DIH) except that no lens is used to collimate the object beam. The laser beams are expanded with an objective lens and a spatial filter. This eliminates two lenses from the typical optical path used for in-line holography, which results in a much cleaner hologram recording. The DMH was used for drop size and velocity measurements of the breakup of aerated liquid jets because it is unaffected by the non-spherical droplets that are encountered very close to the injector exit, which would cause problems for techniques such as Phase Doppler Particle Analyzer, otherwise. Large field of view was obtained by patching several high resolution holograms. Droplet velocities in three dimensions were measured by tracking their displacements in the streamwise and cross-stream direction and by tracking the change in the plane of focus in the spanwise direction. The uncertainty in spanwise droplet location and velocity measurements using single view DMH was large at least 33

  1. Light-Front Holography and Non-Perturbative QCD

    SciTech Connect

    Brodsky, Stanley J.; de Teramond, Guy F.; /Costa Rica U.

    2009-12-09

    The combination of Anti-de Sitter space (AdS) methods with light-front holography leads to a semi-classical first approximation to the spectrum and wavefunctions of meson and baryon light-quark bound states. Starting from the bound-state Hamiltonian equation of motion in QCD, we derive relativistic light-front wave equations in terms of an invariant impact variable {zeta} which measures the separation of the quark and gluonic constituents within the hadron at equal light-front time. These equations of motion in physical space-time are equivalent to the equations of motion which describe the propagation of spin-J modes in anti-de Sitter (AdS) space. Its eigenvalues give the hadronic spectrum, and its eigenmodes represent the probability distribution of the hadronic constituents at a given scale. Applications to the light meson and baryon spectra are presented. The predicted meson spectrum has a string-theory Regge form M{sup 2} = 4{kappa}{sup 2}(n + L + S = 2); i.e., the square of the eigenmass is linear in both L and n, where n counts the number of nodes of the wavefunction in the radial variable {zeta}. The space-like pion form factor is also well reproduced. One thus obtains a remarkable connection between the description of hadronic modes in AdS space and the Hamiltonian formulation of QCD in physical space-time quantized on the light-front at fixed light-front time {tau}. The model can be systematically improved by using its complete orthonormal solutions to diagonalize the full QCD light-front Hamiltonian or by applying the Lippmann-Schwinger method in order to systematically include the QCD interaction terms.

  2. A Study Guide on Holography (Draft). Test Edition. AAAS Study Guides on Contemporary Problems.

    ERIC Educational Resources Information Center

    Jeong, Tung H.

    This is one of several study guides on contemporary problems produced by the American Association for the Advancement of Science with support of the National Science Foundation. The primary purpose of this guide is to provide a student with sufficient practical and technical information to begin independently practicing holography, with occasional…

  3. The effect of object motion in Fraunhofer holography with application to velocity measurements

    NASA Technical Reports Server (NTRS)

    Dotson, W. P., Jr.

    1970-01-01

    Experimental results extend the Fraunhofer holography theory to include moving objects. Conclusions indicate objects may move up to ten times their mean diameter during observation time. Their motion produces fringe patterns descriptive of that motion from which it is possible to reconstruct the hologram and measure the velocity.

  4. Parallel-quadrature phase-shifting digital holographic microscopy using polarization beam splitter

    PubMed Central

    Das, Bhargab; Yelleswarapu, Chandra S; Rao, DVGLN

    2012-01-01

    We present a digital holography microscopy technique based on parallel-quadrature phase-shifting method. Two π/2 phase-shifted holograms are recorded simultaneously using polarization phase-shifting principle, slightly off-axis recording geometry, and two identical CCD sensors. The parallel phase-shifting is realized by combining circularly polarized object beam with a 45° degree polarized reference beam through a polarizing beam splitter. DC term is eliminated by subtracting the two holograms from each other and the object information is reconstructed after selecting the frequency spectrum of the real image. Both amplitude and phase object reconstruction results are presented. Simultaneous recording eliminates phase errors caused by mechanical vibrations and air turbulences. The slightly off-axis recording geometry with phase-shifting allows a much larger dimension of the spatial filter for reconstruction of the object information. This leads to better reconstruction capability than traditional off-axis holography. PMID:23109732

  5. Parallel-quadrature phase-shifting digital holographic microscopy using polarization beam splitter.

    PubMed

    Das, Bhargab; Yelleswarapu, Chandra S; Rao, Dvgln

    2012-11-01

    We present a digital holography microscopy technique based on parallel-quadrature phase-shifting method. Two π/2 phase-shifted holograms are recorded simultaneously using polarization phase-shifting principle, slightly off-axis recording geometry, and two identical CCD sensors. The parallel phase-shifting is realized by combining circularly polarized object beam with a 45° degree polarized reference beam through a polarizing beam splitter. DC term is eliminated by subtracting the two holograms from each other and the object information is reconstructed after selecting the frequency spectrum of the real image. Both amplitude and phase object reconstruction results are presented. Simultaneous recording eliminates phase errors caused by mechanical vibrations and air turbulences. The slightly off-axis recording geometry with phase-shifting allows a much larger dimension of the spatial filter for reconstruction of the object information. This leads to better reconstruction capability than traditional off-axis holography.

  6. Sandia Particle Holography Processor v. 1.0

    SciTech Connect

    Guildenbecher, Daniel

    2015-12-09

    HoloSand is a suite of MATLAB® functions for measurement of particle properties from digital holograms. Functions to read and display digital holograms are included along with scripts to extract three-dimensional particle positions, sizes, and velocities. This software is a research tool used to process experimental data. A user of this software would first record digital holograms of a particle field and then use this software to measure the three-dimensional particle information encoded in the hologram. Applications range from basic laboratory fluids research to applied particle measurements. The software has no known military applications. The software solves the diffraction integral equation to numerically refocus digital holograms and then applies the hybrid and cross-correlation methods described in the references to extract three-dimensional particle information. User input is required to read-in the hologram and select optimum settings for image segmentation.

  7. Sandia Particle Holography Processor v. 1.0

    2015-12-09

    HoloSand is a suite of MATLAB® functions for measurement of particle properties from digital holograms. Functions to read and display digital holograms are included along with scripts to extract three-dimensional particle positions, sizes, and velocities. This software is a research tool used to process experimental data. A user of this software would first record digital holograms of a particle field and then use this software to measure the three-dimensional particle information encoded in the hologram.more » Applications range from basic laboratory fluids research to applied particle measurements. The software has no known military applications. The software solves the diffraction integral equation to numerically refocus digital holograms and then applies the hybrid and cross-correlation methods described in the references to extract three-dimensional particle information. User input is required to read-in the hologram and select optimum settings for image segmentation.« less

  8. Digital Citizenship

    ERIC Educational Resources Information Center

    Isman, Aytekin; Canan Gungoren, Ozlem

    2014-01-01

    Era in which we live is known and referred as digital age.In this age technology is rapidly changed and developed. In light of these technological advances in 21st century, schools have the responsibility of training "digital citizen" as well as a good citizen. Digital citizens must have extensive skills, knowledge, Internet and …

  9. Numerical simulation of acoustic holography with propagator adaptation. Application to a 3D disc

    NASA Astrophysics Data System (ADS)

    Martin, Vincent; Le Bourdon, Thibault; Pasqual, Alexander Mattioli

    2011-08-01

    Acoustical holography can be used to identify the vibration velocity of an extended vibrating body. Such an inverse problem relies on the radiated acoustic pressure measured by a microphone array and on an a priori knowledge of the way the body radiates sound. Any perturbation on the radiation model leads to a perturbation on the velocity identified by the inversion process. Thus, to obtain the source vibration velocity with a good precision, it is useful to identify also an appropriate propagation model. Here, this identification, or adaptation, procedure rests on a geometrical interpretation of the acoustic holography in the objective space (here the radiated pressure space equipped with the L2-norm) and on a genetic algorithm. The propagator adaptation adds information to the holographic process, so it is not a regularisation method, which approximates the inverse of the model but does not affect the model. Moreover regularisations act in the variables space, here the velocities space. It is shown that an adapted model significantly decreases the quantity of regularisation needed to obtain a good reconstructed velocity, and that model adaptation improves significantly the acoustical holography results. In the presence of perturbations on the radiated pressure, some indications will be given on the interest or not to adapt the model, again thanks to the geometrical interpretation of holography in the objective space. As a numerical example, a disc whose vibration velocity on one of its sides is identified by acoustic holography is presented. On an industrial scale, this problem occurs due to the noise radiated by car wheels. The assessment of the holographic results has not yet been rigorously performed in such situations due to the complexity of the wheel environment made up of the car body, road and rolling conditions.

  10. Micro-electro-fluidic grids for nematodes: A lens-less, image-sensor-less approach for on-chip tracking of nematode locomotion

    PubMed Central

    Liu, Peng; Martin, Richard J.; Dong, Liang

    2013-01-01

    This paper reports on the development of a lens-less and image-sensor-less micro-electro-fluidic (MEF) approach for real-time monitoring of the locomotion of microscopic nematodes. The technology showed promise for overcoming the constraint of the limited field of view of conventional optical microscopy, with a relatively low cost, good spatial resolution, and high portability. The core of the device was microelectrode grids formed by orthogonally arranging two identical arrays of microelectrode lines. The two microelectrode arrays were spaced by a microfluidic chamber containing a liquid medium of interest. As a nematode (e.g., Caenorhabditis elegans) moved inside the chamber, the invasion of its body parts into some intersection regions between the microelectrodes caused changes in electrical resistance of these intersection regions. The worm's presence at or absence from a detection unit was determined by a comparison between the measured resistance variation of this unit and a pre-defined threshold resistance variation. An electronic readout circuit was designed to address all detection units and read out their individual electrical resistance. By this means, it was possible to obtain the electrical resistance profile of the whole MEF grids, and thus, the physical pattern of the swimming nematode. We studied the influence of a worm's body on the resistance of an addressed unit. We also investigated how the full-frame scanning and readout rate of the electronic circuit and the dimensions of a detection unit posed an impact on the spatial resolution of the reconstructed images of the nematode. Other important issues, such as the manufacturing induced initial non-uniformity of the grids and the electrotaxic behaviour of nematodes, were also studied. A drug resistance screening experiment was conducted by using the grids with a good resolution of 30 × 30 μm2. The phenotypic differences in the locomotion behaviours (e.g., moving speed and oscillation frequency

  11. Digital Natives or Digital Tribes?

    ERIC Educational Resources Information Center

    Watson, Ian Robert

    2013-01-01

    This research builds upon the discourse surrounding digital natives. A literature review into the digital native phenomena was undertaken and found that researchers are beginning to identify the digital native as not one cohesive group but of individuals influenced by other factors. Primary research by means of questionnaire survey of technologies…

  12. High-intensity x-ray holography: an approach to high-resolution snapshot imaging of biological specimens

    SciTech Connect

    Solem, J.C.

    1982-08-01

    The crucial physical and technological issues pertaining to the holographic imaging of biological structures with a short-pulse, high-intensity, high-quantum-energy laser were examined. The limitations of x-ray optics are discussed. Alternative holographic techniques were considered, and it was concluded that far-field Fresnel transform holography (Fraunhofer holography) using a photoresist recording surface is most tractable with near term technology. The hydrodynamic expansion of inhomogeneities within the specimen is discussed. It is shown that expansion is the major source of image blurring. Analytic expressions were derived for the explosion of protein concentrations in an x-ray transparent cytoplasm, compared with numerical calculations, and corrections derived to account for the competitive transport processes by which these inhomogeneities lose energy. It is concluded that for the near term Fresnel transform holography, particularly, far-field or Fraunhofer holography, is more practical than Fourier transform holography. Of the alternative fine grain recording media for use with Fresnel transform holography, a photo-resist is most attractive. For best resolution, exposure times must be limited to a few picoseconds, and this calls for investigation of mechanisms to shutter the laser or gate the recording surface. The best contrast ratio between the nitrogen-bearing polymers (protein and the nucleic acids) and water is between the K-edges of oxygen and nitrogen.

  13. Submicron Defect Detection in Periodic Structures Using Photorefractive Holography

    NASA Astrophysics Data System (ADS)

    Uhrich, Craig Edward

    Detection of defects in periodic objects is an important step in the manufacture of integrated circuits, particularly memory chips which are highly repetitive. As feature sizes shrink below 1 mum, automated detection of sub-micron defects becomes necessary. We present a real-time holographic system which allows the detection of sub-micron defects in periodic structures. The inspection technique is a marriage of Fourier spatial filtering and real-time phase conjugate holography. The system output is a near diffraction limited image of any defects in the pattern (defined to be any deviations from periodicity). Real-time holographic recording allows the system to adapt to the orientation and period of the object. The early chapters introduce the photorefractive effect and the generation of the phase conjugate of an optical wavefront using Bi_{12} SiO_{20}. Relevant properties of the optical Fourier transform are also discussed. The following sections present the system design rules and experimental techniques which allow the system to reliably detect sub-micron defects. The most important improvements over previous demonstrations are (1) a novel write/read holographic process which increases phase conjugate reflectivity by orders of magnitude, (2) control of the light polarization to allow efficient object illumination and collection of the diffracted signal, (3) avoidance of noise by moving the crystal away from the origin of the Fourier plane, and (4) compensation for the crystal aberrations with a holographic optical element. The final chapter presents results which clearly show enhancement of sub-micron defects and near perfect suppression of the surrounding periodic patterns. An area larger than 1 mm^2 is inspected in 20-60 seconds. Over 90% of the 0.5 mu m diameter defects on glass masks (viewed in reflection) are detected, and approximately 80% of defects in the 0.2 -0.3 μm range are found. On silicon wafers, the results are even better with over a 95% success

  14. In-line digital holographic imaging in volume holographic microscopy.

    PubMed

    Zhai, Xiaomin; Lin, Wei-Tang; Chen, Hsi-Hsun; Wang, Po-Hao; Yeh, Li-Hao; Tsai, Jui-Chang; Singh, Vijay Raj; Luo, Yuan

    2015-12-01

    A dual-plane in-line digital holographic imaging method incorporating volume holographic microscopy (VHM) is presented to reconstruct objects in a single shot while eliminating zero-order and twin-image diffracted waves. The proposed imaging method is configured such that information from different axial planes is acquired simultaneously using multiplexed volume holographic imaging gratings, as used in VHM, and recorded as in-line holograms where the corresponding reference beams are generated in the fashion of Gabor's in-line holography. Unlike conventional VHM, which can take axial intensity information only at focal depths, the proposed method digitally reconstructs objects at any axial position. Further, we demonstrate the proposed imaging technique's ability to effectively eliminate zero-order and twin images for single-shot three-dimensional object reconstruction. PMID:26625046

  15. Rapid detection of malignant bio-species using digital holographic pattern recognition and nano-photonics

    NASA Astrophysics Data System (ADS)

    Sarkisov, Sergey S.; Kukhtareva, Tatiana; Kukhtarev, Nickolai V.; Curley, Michael J.; Edwards, Vernessa; Creer, Marylyn

    2013-03-01

    There is a great need for rapid detection of bio-hazardous species particularly in applications to food safety and biodefense. It has been recently demonstrated that the colonies of various bio-species could be rapidly detected using culture-specific and reproducible patterns generated by scattered non-coherent light. However, the method heavily relies on a digital pattern recognition algorithm, which is rather complex, requires substantial computational power and is prone to ambiguities due to shift, scale, or orientation mismatch between the analyzed pattern and the reference from the library. The improvement could be made, if, in addition to the intensity of the scattered optical wave, its phase would be also simultaneously recorded and used for the digital holographic pattern recognition. In this feasibility study the research team recorded digital Gabor-type (in-line) holograms of colonies of micro-organisms, such as Salmonella with a laser diode as a low-coherence light source and a lensless high-resolution (2.0x2.0 micron pixel pitch) digital image sensor. The colonies were grown in conventional Petri dishes using standard methods. The digitally recorded holograms were used for computational reconstruction of the amplitude and phase information of the optical wave diffracted on the colonies. Besides, the pattern recognition of the colony fragments using the cross-correlation between the digital hologram was also implemented. The colonies of mold fungi Altenaria sp, Rhizophus, sp, and Aspergillus sp have been also generating nano-colloidal silver during their growth in specially prepared matrices. The silver-specific plasmonic optical extinction peak at 410-nm was also used for rapid detection and growth monitoring of the fungi colonies.

  16. Phase reference holography of the Noto 32 m VLBI antenna

    NASA Astrophysics Data System (ADS)

    Tuccari, G.; Buttaccio, S.; Nicotra, G.

    2002-08-01

    Holographic measurements to determine deviations of the 32m Noto antenna with respect to an ideal parabolic surface have been performed at 11.5 GHz. An `ad hoc' system including a reference secondary antenna was built, and digital beacons coming from commercial TV satellites were used as signal sources. Results clearly showed significant deformations of the dish

  17. Digital printing

    NASA Astrophysics Data System (ADS)

    Sobotka, Werner K.

    1997-02-01

    Digital printing is described as a tool to replace conventional printing machines completely. Still this goal was not reached until now with any of the digital printing technologies to be described in the paper. Productivity and costs are still the main parameters and are not really solved until now. Quality in digital printing is no problem anymore. Definition of digital printing is to transfer digital datas directly on the paper surface. This step can be carried out directly or with the use of an intermediate image carrier. Keywords in digital printing are: computer- to-press; erasable image carrier; image carrier with memory. Digital printing is also the logical development of the new digital area as it is pointed out in Nicholas Negropotes book 'Being Digital' and also the answer to networking and Internet technologies. Creating images text and color in one country and publishing the datas in another country or continent is the main advantage. Printing on demand another big advantage and last but not least personalization the last big advantage. Costs and being able to coop with this new world of prepress technology is the biggest disadvantage. Therefore the very optimistic growth rates for the next few years are really nonexistent. The development of complete new markets is too slow and the replacing of old markets is too small.

  18. Digital metamaterials.

    PubMed

    Della Giovampaola, Cristian; Engheta, Nader

    2014-12-01

    Balancing complexity and simplicity has played an important role in the development of many fields in science and engineering. One of the well-known and powerful examples of such balance can be found in Boolean algebra and its impact on the birth of digital electronics and the digital information age. The simplicity of using only two numbers, '0' and '1', in a binary system for describing an arbitrary quantity made the fields of digital electronics and digital signal processing powerful and ubiquitous. Here, inspired by the binary concept, we propose to develop the notion of digital metamaterials. Specifically, we investigate how one can synthesize an electromagnetic metamaterial with a desired permittivity, using as building blocks only two elemental materials, which we call 'metamaterial bits', with two distinct permittivity functions. We demonstrate, analytically and numerically, how proper spatial mixtures of such metamaterial bits lead to elemental 'metamaterial bytes' with effective material parameters that are different from the parameters of the metamaterial bits. We then apply this methodology to several design examples of optical elements, such as digital convex lenses, flat graded-index digital lenses, digital constructs for epsilon-near-zero (ENZ) supercoupling and digital hyperlenses, thus highlighting the power and simplicity of the methodology.

  19. Two versions of holography vie to show atoms in 3D

    SciTech Connect

    Nadis, S.

    1996-05-03

    In 1948 Dennis Gabor had an idea nearly 50 years ahead of his time, laying the theoretical groundwork for holography, a technique for making three dimensional images from interference patterns created when beams of coherent light or other radiation are shined on the object. Two research groups have developed holography on an atomic scale. One group from Budapest used atoms within a specimen itself as sources of coherent x-rays, a hologram can be made inside a solid - a crystal of the mineral perovskite, and by using atoms to detect rather than generate signals another groups has achieved an atomic-scale hologram. This article discusses the development and progress of the research.

  20. Incoherent holography by a Michelson type interferometer with a lens for a radial shear

    NASA Astrophysics Data System (ADS)

    Watanabe, Kaho; Nomura, Takanori

    2016-06-01

    The modified Michelson type interferometer with lenses for a radial shear to record incoherent holograms is proposed. It enables us to record a hologram by self-interference without coherent illumination such as a laser. The interferometer has two wave plates which can realize phase-shifting incoherent holography. The feature can avoid a very large bias term and the twin image, which are the inherent problem of incoherent holography by self-interference. The advantages of the proposed method using lenses and wave plates are easy adjustment of the zone plate and simplification of the optical system. A preliminary experiment using an LED as an incoherent object was performed to confirm the four step phase-shifting by wave plates.