Sample records for leopardus pardalis submitted

  1. Survey of feline leukemia virus and feline coronaviruses in captive neotropical wild felids from Southern Brazil.

    PubMed

    Guimaraes, Ana M S; Brandão, Paulo E; de Moraes, Wanderlei; Cubas, Zalmir S; Santos, Leonilda C; Villarreal, Laura Y B; Robes, Rogério R; Coelho, Fabiana M; Resende, Mauricio; Santos, Renata C F; Oliveira, Rosangela C; Yamaguti, Mauricio; Marques, Lucas M; Neto, Renata L; Buzinhani, Melissa; Marques, Regina; Messick, Joanne B; Biondo, Alexander W; Timenetsky, Jorge

    2009-06-01

    A total of 57 captive neotropical felids (one Leopardus geoffroyi, 14 Leopardus pardalis, 17 Leopardus wiedii, 22 Leopardus tigrinus, and three Puma yagouaroundi) from the Itaipu Binacional Wildlife Research Center (Refúgio Bela Vista, Southern Brazil) were anesthetized for blood collection. Feces samples were available for 44 animals, including one L. geoffroyi, eight L. pardalis, 14 L. wiedii, 20 L. tigrinus, and one P. yagouaroundi. Total DNA and RNA were extracted from blood and feces, respectively, using commercial kits. Blood DNA samples were evaluated by polymerase chain reaction (PCR) for feline leukemia virus (FeLV) proviral DNA, whereas reverse transcriptase-PCR was run on fecal samples for detection of coronavirus RNA. None of the samples were positive for coronaviruses. A male L. pardalis and a female L. tigrinus were positive for FeLV proviral DNA, and identities of PCR products were confirmed by sequencing. This is the first evidence of FeLV proviral DNA in these species in Southern Brazil.

  2. Ocelot (Leopardus pardalis) Density in Central Amazonia.

    PubMed

    Rocha, Daniel Gomes da; Sollmann, Rahel; Ramalho, Emiliano Esterci; Ilha, Renata; Tan, Cedric K W

    2016-01-01

    Ocelots (Leopardus pardalis) are presumed to be the most abundant of the wild cats throughout their distribution range and to play an important role in the dynamics of sympatric small-felid populations. However, ocelot ecological information is limited, particularly for the Amazon. We conducted three camera-trap surveys during three consecutive dry seasons to estimate ocelot density in Amanã Reserve, Central Amazonia, Brazil. We implemented a spatial capture-recapture (SCR) model that shared detection parameters among surveys. A total effort of 7020 camera-trap days resulted in 93 independent ocelot records. The estimate of ocelot density in Amanã Reserve (24.84 ± SE 6.27 ocelots per 100 km2) was lower than at other sites in the Amazon and also lower than that expected from a correlation of density with latitude and rainfall. We also discuss the importance of using common parameters for survey scenarios with low recapture rates. This is the first density estimate for ocelots in the Brazilian Amazon, which is an important stronghold for the species.

  3. Serological Detection of Viral Infections in Captive Wild Cats from Costa Rica

    PubMed Central

    Blanco, Kinndle; Peña, Roberto; Hernández, Carmen; Jiménez, Mauricio; Araya, Luis Nazario; Romero, Juan José; Dolz, Gaby

    2011-01-01

    Serum samples from a total of 44 wildcats, 28 margays (Leopardus wiedii), 10 ocelots (Leopardus pardalis), four jaguaroundis (Herpailurus yaguaroundi), one oncilla (Leopardus tigrina), and one jaguar (Panthera onca) were obtained between January 2001 and August 2002 from the Profelis Centre for rehabilitation of wild felids, located in the northwestern region of Costa Rica. Forty three samples were tested for antibodies against feline immunodeficiency virus (FIV) and p27 antigen of feline leukemia virus (FeLV), 42 samples for antibodies against feline parvovirus (FPV), and 30 for antibodies against feline calicivirus (FCV). None of the samples contained detectable antibodies against FIV or p27 antigen of FeLV, all samples contained antibodies against FPV, and one sample contained antibodies against FCV. PMID:21547230

  4. Serological detection of viral infections in captive wild cats from costa rica.

    PubMed

    Blanco, Kinndle; Peña, Roberto; Hernández, Carmen; Jiménez, Mauricio; Araya, Luis Nazario; Romero, Juan José; Dolz, Gaby

    2011-04-03

    Serum samples from a total of 44 wildcats, 28 margays (Leopardus wiedii), 10 ocelots (Leopardus pardalis), four jaguaroundis (Herpailurus yaguaroundi), one oncilla (Leopardus tigrina), and one jaguar (Panthera onca) were obtained between January 2001 and August 2002 from the Profelis Centre for rehabilitation of wild felids, located in the northwestern region of Costa Rica. Forty three samples were tested for antibodies against feline immunodeficiency virus (FIV) and p27 antigen of feline leukemia virus (FeLV), 42 samples for antibodies against feline parvovirus (FPV), and 30 for antibodies against feline calicivirus (FCV). None of the samples contained detectable antibodies against FIV or p27 antigen of FeLV, all samples contained antibodies against FPV, and one sample contained antibodies against FCV.

  5. 75 FR 52547 - Endangered and Threatened Wildlife and Plants; Draft Ocelot (Leopardus pardalis

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-26

    ... populations of the ocelot in the borderlands of the United States and Mexico; Reduction of effects of human population growth and development to ocelot survival and mortality; Maintenance or improvement of genetic... are necessary for ocelot dispersal. In south Texas, 2 remaining ocelot populations of less than 25...

  6. Canine Distemper Virus in Wild Felids of Costa Rica.

    PubMed

    Avendaño, Roberto; Barrueta, Flor; Soto-Fournier, Sofía; Chavarría, Max; Monge, Otto; Gutiérrez-Espeleta, Gustavo A; Chaves, Andrea

    2016-04-28

    Several highly infectious diseases can be transmitted through feces and cause elevated mortality among carnivore species. One such infectious agent, canine distemper virus (CDV; Paramyxoviridae: Morbillivirus), has been reported to affect wild carnivores, among them several felid species. We screened free-ranging and captive wild carnivores in Costa Rica for CDV. Between 2006 and 2012, we collected 306 fecal samples from 70 jaguars (Panther onca), 71 ocelots ( Leopardus pardalis ), five jaguarundis (Puma yaguaroundi), 105 pumas ( Puma concolor ), five margays ( Leopardus wiedii ), 23 coyotes ( Canis latrans ), and 27 undetermined Leopardus spp. We found CDV in six individuals: one captive jaguarundi (rescued in 2009), three free-ranging ocelots (samples collected in 2012), and two free-ranging pumas (samples collected in 2007). Phylogenetic analyses were performed using sequences of the phosphoprotein (P) gene. We provide evidence of CDV in wild carnivores in Costa Rica and sequence data from a Costa Rican CDV isolate, adding to the very few sequence data available for CDV isolates from wild Central American carnivores.

  7. Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland.

    PubMed

    de Sousa, Keyla Carstens Marques; Herrera, Heitor Miraglia; Secato, Caroline Tostes; Oliveira, André do Vale; Santos, Filipe Martins; Rocha, Fabiana Lopes; Barreto, Wanessa Teixeira Gomes; Macedo, Gabriel Carvalho; de Andrade Pinto, Pedro Cordeiro Estrela; Machado, Rosangela Zacarias; Costa, Mirela Tinucci; André, Marcos Rogério

    2017-07-01

    Hemotropic mycoplasmas are known to cause anemia in several mammalian species. The present work aimed to investigate the occurrence of Mycoplasma spp. in wild mammals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil. Between August 2013 and March 2015, 31 Nasua nasua, 78 Cerdocyon thous, seven Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials were trapped and ectoparasites (ticks and fleas) found parasitizing the animals were collected. Mammals and ectoparasites DNA samples were submitted to conventional PCR assays for Mycoplasma spp. targeting 16S rRNA and RnaseP genes. Twenty-four N. nasua, three C. thous, two domestic dogs, one L. pardalis and one wild rodent were positive for 16S rRNA PCR protocols. Fourteen N. nasua samples were also positive in RnaseP PCR. No marsupial or arthropod showed positivity for Mycoplasma spp. The phylogenetic analyses based on 16S rRNA gene showed that all sequences obtained from dogs, two sequences obtained from C. thous and ten sequences obtained from N. nasua showed to be closely related to Mycoplasma haemocanis/Mycoplasma haemofelis species. Genotypes closely related to 'Candidatus Mycoplasma haemominutum' and Mycoplasma haemomuris were detected in the L. pardalis and in the wild rodent, respectively. Probably a novel Mycoplasma genotype, closely related to a sequence obtained from a Brazilian capybara was detected in 14 N. nasua, based on a concatenated phylogenetic analysis of 16S rRNA and RnaseP genes. The present study revealed that wild animals in southern Pantanal region, Brazil, are exposed to different species of hemoplasmas. Copyright © 2017 Elsevier B.V. All rights reserved.

  8. Noninvasive individual and species identification of jaguars (Panthera onca), pumas (Puma concolor) and ocelots (Leopardus pardalis) in Belize, Central America using cross-species microsatellites and faecal DNA.

    PubMed

    Wultsch, Claudia; Waits, Lisette P; Kelly, Marcella J

    2014-11-01

    There is a great need to develop efficient, noninvasive genetic sampling methods to study wild populations of multiple, co-occurring, threatened felids. This is especially important for molecular scatology studies occurring in challenging tropical environments where DNA degrades quickly and the quality of faecal samples varies greatly. We optimized 14 polymorphic microsatellite loci for jaguars (Panthera onca), pumas (Puma concolor) and ocelots (Leopardus pardalis) and assessed their utility for cross-species amplification. Additionally, we tested their reliability for species and individual identification using DNA from faeces of wild felids detected by a scat detector dog across Belize in Central America. All microsatellite loci were successfully amplified in the three target species, were polymorphic with average expected heterozygosities of HE = 0.60 ± 0.18 (SD) for jaguars, HE = 0.65 ± 0.21 (SD) for pumas and HE = 0.70 ± 0.13 (SD) for ocelots and had an overall PCR amplification success of 61%. We used this nuclear DNA primer set to successfully identify species and individuals from 49% of 1053 field-collected scat samples. This set of optimized microsatellite multiplexes represents a powerful tool for future efforts to conduct noninvasive studies on multiple, wild Neotropical felids. © 2014 John Wiley & Sons Ltd.

  9. Variability in assays used for detection of lentiviral infection in bobcats (Lynx rufus), pumas (Puma concolor), and ocelots (Leopardus pardalis)

    USGS Publications Warehouse

    Franklin, S.P.; Troyer, J.L.; TerWee, J.A.; Lyren, L.M.; Kays, R.W.; Riley, S.P.D.; Boyce, W.M.; Crooks, K.R.; VandeWoude, S.

    2007-01-01

    Although lentiviruses similar to feline immunodeficiency virus (FIV) are known to infect numerous felid species, the relative utility of assays used for detecting lentiviral infection has not been compared for many of these hosts. We tested bobcats (Lynx rufus), pumas (Felis concolor), and ocelots (Leopardus pardalis) for exposure to lentivirus using five different assays: puma lentivirus (PLV), African lion lentivirus (LLV), and domestic cat FIV-based immunoblots, a commercially available enzyme-linked immunosorbent assay (ELISA) kit, and nested polymerase chain reaction (PCR). Puma lentivirus immunoblots identified more seropositive individuals than the other antibody-detection assays. The commercial ELISA provided a fair ability to recognize seropositive samples when compared with PLV immunoblot for screening bobcats and ocelots, but not pumas. Polymerase chain reaction identified fewer positive samples than PLV immunoblot for all three species. Immunoblot results were equivalent whether the sample tested was serum, plasma, or whole blood. The results from this study and previous investigations suggest that the PLV immunoblot has the greatest ability to detect reactive samples when screening wild felids of North America and is unlikely to produce false positive results. However, the commercial ELISA kit may provide ap adequate alternative for screening of some species and is more easily adapted to field conditions. ?? Wildlife Disease Association 2007.

  10. Novel piroplasmid and Hepatozoon organisms infecting the wildlife of two regions of the Brazilian Amazon.

    PubMed

    Soares, Herbert S; Marcili, Arlei; Barbieri, Amália R M; Minervino, Antonio H H; Moreira, Thiago Rocha; Gennari, Solange M; Labruna, Marcelo B

    2017-08-01

    During 2009-2012, wild animals were sampled in two areas within the Amazon biome of Brazil, in the states of Mato Grosso and Pará. Animal tissues and blood were molecularly tested for the presence of Piroplasmida (genera Babesia, Theileria, Cytauxzoon ) or Hepatozoon DNA. Overall, 181 wild animals comprising 36 different species (2 reptiles, 5 birds, and 29 mammals) were sampled. The following Piroplasmida agents were detected: Cytauxzoon felis in one ocelot ( Leopardus pardalis ), Theileria cervi in two red brocket deer ( Mazama americana ), Theileria spp. in three nine-banded-armadillos ( Dasypus novemcinctus ), one agouti ( Dasyprocta sp.), and four lowland pacas ( Cuniculus paca ), Babesia spp. in one common opossum ( Didelphis marsupialis ) and one white-lipped peccary ( Tayassu pecari ). The following Hepatozoon agents were detected: Hepatozoon sp. (possibly Hepatozoon caimani ) in three spectacled caimans ( Caiman crocodilus ), Hepatozoon felis in an ocelot ( Leopardus pardalis ), and Hepatozoon spp. in one scorpion mud turtle ( Kinosternon scorpioides ) and one lowland paca ( Cuniculus paca ). Phylogenetic analyses inferred by the 18S rRNA gene partial sequences supported these results, highlighting at least five novel Piroplasmida agents, and two novel Hepatozoon agents. This study screened the presence of tick-borne protozoa in a number of wildlife species from the Amazon for the first time. Our results indicate that a variety of genetically distinct Piroplasmida and Hepatozoon organisms circulate under natural conditions in the Amazonian wildlife.

  11. Camera-trap study of ocelot and other secretive mammals in the northern Pantanal

    USGS Publications Warehouse

    Trolle, M.; Kery, M.

    2005-01-01

    Reliable information on abundance of the ocelot (Leopardus pardalis) is scarce. We conducted the first camera-trap study in the northern part of the Pantanal wetlands of Brazil, one of the wildlife hotspots of South America. Using capture-recapture analysis, we estimated a density of 0.112 independent individuals per km2 (SE 0.069). We list other mammals recorded with camera traps and show that camera-trap placement on roads or on trails has striking effects on camera-trapping rates.

  12. Gastrointestinal parasites among felids inhabiting the Serra dos Órgãos National Park, Rio de Janeiro, Brazil.

    PubMed

    Dib, Laís Verdan; Cronemberger, Cecília; Pereira, Fabiane de Aguiar; Bolais, Paula Forain; Uchôa, Claudia Maria Antunes; Bastos, Otilio Machado Pereira; Amendoeira, Maria Regina Reis; Barbosa, Alynne da Silva

    2018-05-24

    This study aimed to investigate the species of felids that inhabit the Serra dos Órgãos National Park (Parnaso) and gastrointestinal parasites at various stages of their life cycles in the feces of these animals. Between 2013 and 2015, felid feces were collected from trails in Parnaso. The sampling points were georeferenced. A total of 82 fecal samples were processed, of which 79 were collected on the ground, two from captured felids and one from a necropsied animal. All samples underwent coproparasitological techniques. Samples collected from the environment underwent additional trichological analysis. Fur patterns corresponding to Leopardus guttulus, Leopardus pardalis, Leopardus wiedii and Puma yagouaroundi were observed in 32 of the samples collected on the soil. High frequency of potentially parasitic evolving forms (88.6%) was observed in felid feces, particularly eggs of the family Diphyllobothriidae (68.6%). Besides, were also detected, eggs of superfamily Ascaridoidea (42.9%), nematode larvae (28.6%), eggs of order Strongylida (28.6%), Capillaria sp. (8.6%), Trichuris sp. (8.6%), eggs of order Spirurida (2.9%), unsporulated coccidian oocysts (8.6%) and Eimeria sp. (2.9%). Felid feces presented higher frequency of polyparasitism (60%) than monoparasitism (28.6%).

  13. Molecular detection of Hepatozoon spp. in domestic dogs and wild mammals in southern Pantanal, Brazil with implications in the transmission route.

    PubMed

    de Sousa, Keyla Carstens Marques; Fernandes, Marina Pugnaghi; Herrera, Heitor Miraglia; Benevenute, Jyan Lucas; Santos, Filipe Martins; Rocha, Fabiana Lopes; Barreto, Wanessa Teixeira Gomes; Macedo, Gabriel Carvalho; Campos, João Bosco; Martins, Thiago Fernandes; de Andrade Pinto, Pedro Cordeiro Estrela; Battesti, Darci Barros; Piranda, Eliane Mattos; Cançado, Paulo Henrique Duarte; Machado, Rosangela Zacarias; André, Marcos Rogério

    2017-04-15

    Hepatozoon parasites comprise intracellular apicomplexan parasites transmitted to vertebrate animals by ingestion of arthropods definitive hosts. The present work aimed to investigate the occurrence of Hepatozoon spp. in wild animals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil, by molecular techniques. Between August 2013 and March 2015, 31 coatis (Nasua nasua), 78 crab-eating foxes (Cerdocyon thous), seven ocelots (Leopardus pardalis), 42 dogs (Canis lupus familiaris), 110 wild rodents (77 Thichomys fosteri, 25 Oecomys mamorae, and 8 Clyomys laticeps), 30 marsupials (14 Thylamys macrurus, 11 Gracilinanus agilis, 4 Monodelphis domestica and 1 Didelphis albiventris), and 1582 ticks and 80 fleas collected from the sampled animals were investigated. DNA samples were submitted to PCR assays for Hepatozoon spp. targeting 18S rRNA gene. Purified amplicons were directly sequenced and submitted to phylogenetic analysis. A high prevalence of Hepatozoon among carnivores (C. thous [91.02%], dogs [45.23%], N. nasua [41.9%] and L. pardalis [71.4%]) was found. However, ticks and fleas were negative to Hepatozoon PCR assays. By phylogenetic analysis based on 18S rRNA sequences, Hepatozoon sequences amplified from crab-eating foxes, dogs, coatis and ocelots clustered with sequences of H. canis, H. americanum and H. felis. The closely related positioning of Hepatozoon sequences amplified from wild rodents and T. macrurus marsupial to Hepatozoon from reptiles and amphibians suggest a possible transmission of those Hepatozoon species between hosts by ectoparasites or by predation. Hepatozoon haplotypes found circulating in wild rodents seem to present a higher degree of polymorphism when compared to those found in other groups of animals. Although rodents seem not to participate as source of Hepatozoon infection to wild carnivores and domestic dogs, they may play an important role in the transmission of Hepatozoon to reptiles and amphibians in Pantanal biome. Copyright © 2017 Elsevier B.V. All rights reserved.

  14. Density, distribution, and activity of the ocelot Leopardus pardalis (Carnivora: Felidae) in Southeast Mexican rainforests.

    PubMed

    Pérez-Irineo, Gabriela; Santos-Moreno, Antonio

    2014-12-01

    The ocelot Leopardus pardalis is of particular significance in terrestrial communities due to its ecological role within the group of small-sized felids and as a mesopredator. However, despite the reduction of ocelot habitat in Southeast Mexico, there are still very few ecological studies. This research aimed to contribute with some ecological aspects of the species in this region. For this, 29 camera trap stations were established in a rain forest in Los Chimalapas (an area of 22 km2) during a two years period (March 2011-June, 2013), in Oaxaca state, Southeast Mexico. Data allowed the estimation of the population density, activity pattern, sex ratio, residence time, and spatial distribution. Population density was calculated using Capture-Recapture Models for demographically open populations; besides, circular techniques were used to determine if nocturnal and diurnal activity varied significantly over the seasons, and Multiple Discriminant Analysis was used to determine which of the selected environmental variables best explained ocelot abundance in the region. A total of 103 ocelot records were obtained, with a total sampling effort of 8,529 trap-days. Density of 22-38 individuals/100 km2 was estimated. Ocelot population had a high proportion of transient individuals in the zone (55%), and the sex ratio was statistically equal to 1:1. Ocelot activity was more frequent at night (1:00-6:00h), but it also exhibited diurnal activity throughout the study period. Ocelot spatial distribution was positively affected by the proximity to the village as well as by the amount of prey. The ocelot population here appears to be stable, with a density similar to other regions in Central and South America, which could be attributed to the diversity of prey species and a low degree of disturbance in Los Chimalapas.

  15. Serologic survey for Leptospira spp. in captive neotropical felids in Foz do Iguaçu, Paraná, Brazil.

    PubMed

    Ullmann, Leila Sabrina; Hoffmann, Juliano L; de Moraes, Wanderlei; Cubas, Zalmir S; dos Santos, Leonilda Correia; da Silva, Rodrigo Costa; Moreira, Nei; Guimaraes, Ana Marcia Sa; Camossi, Lucilene Granuzzio; Langoni, Helio; Biondo, Alexander W

    2012-06-01

    Leptospirosis is a bacterial zoonosis of worldwide distribution and is endemic in tropical countries, where rodents and other wild mammals are abundant and may act as reservoirs. Leptospirosis has become a concern in captive wild animals, due mostly to their exposure to contaminated urine or environment. Although domestic cats (Felis catus) have been reported refractory to leptospirosis, serology and disease in captive wild felids is still unclear. In this study 57 adult, clinically healthy felids, including 1 Geoffroy's cat (Leopardus geoffroyi), 3 jaguarundis (Puma yagouaroundi), 17 margays (Leopardus wiedii), 22 little spotted cats (Leopardus tigrinus), and 14 ocelots (Leopardus pardalis) kept in captivity at the Sanctuary at the Itaipu Binacional hydroelectric power plant (Bela Vista Biological Sanctuary), Foz do Iguacu City, Paraná State, Brazil, were serologically surveyed for the presence of antibodies against 28 serovars of Leptospira spp. by microagglutination test (MAT). Two animals (3.5%) were seropositive: one male ocelot to the serovar Cynopteri (titer 100) and one female margay to Autumnalis (100) and Butembo (200). The captive-born, 5-yr-old ocelot had been solitary housed in an individual cage. The approximately 21-yr-old wild-caught margay was also kept individually. None of the tested animals showed signs ofleptospirosis. During a study conducted 4 yr previously in the same facility, this particular margay also tested positive for the same two serovars, among others. The present study indicates that the felids tested for Leptospira spp. by MAT were exposed to serovars, but did not demonstrate clinical signs of disease. Comparison with a previous study suggests that serovar titers may vary over time and that leptospirosis dynamics remains unclear in wild felids.

  16. Ticks (Acari: Ixodida) on wild carnivores in Brazil.

    PubMed

    Labruna, Marcelo B; Jorge, Rodrigo S P; Sana, Dênis A; Jácomo, Anah Tereza A; Kashivakura, Cyntia K; Furtado, Mariana M; Ferro, Claudia; Perez, Samuel A; Silveira, Leandro; Santos, Tarcísio S; Marques, Samuel R; Morato, Ronaldo G; Nava, Alessandra; Adania, Cristina H; Teixeira, Rodrigo H F; Gomes, Albério A B; Conforti, Valéria A; Azevedo, Fernando C C; Prada, Cristiana S; Silva, Jean C R; Batista, Adriana F; Marvulo, Maria Fernanda V; Morato, Rose L G; Alho, Cleber J R; Pinter, Adriano; Ferreira, Patrícia M; Ferreira, Fernado; Barros-Battesti, Darci M

    2005-01-01

    The present study reports field data of ticks infesting wild carnivores captured from July 1998 to September 2004 in Brazil. Additional data were obtained from one tick collection and from previous published data of ticks on carnivores in Brazil. During field work, a total of 3437 ticks were collected from 89 Cerdocyon thous (crab-eating fox), 58 Chrysocyon brachyurus (maned wolf), 30 Puma concolor (puma), 26 Panthera onca (jaguar), 12 Procyon cancrivorus (crab-eating raccoon), 4 Speothos venaticus (bush dog), 6 Pseudalopex vetulus (hoary fox), 6 Nasua nasua (coati), 6 Leopardus pardalis (ocelot), 2 Leopardus tigrinus (oncilla), 1 Leopardus wiedii (margay), 1 Herpailurus yagouaroundi (jaguarundi), 1 Oncifelis colocolo (pampas cat), 1 Eira barbara (tayara), 1 Galictis vittata (grison), 1 Lontra longicaudis (neotropical otter), and 1 Potus flavus (kinkajou). Data obtained from the Acari Collection IBSP included a total of 381 tick specimens collected on 13 C. thous, 8 C. brachyurus, 3 P. concolor, 10 P. onca, 3 P. cancrivorus, 4 N. nasua, 1 L. pardalis, 1 L. wiedii, 4 H. yagouaroundi, 1 Galictis cuja (lesser grison), and 1 L. longicaudis. The only tick-infested carnivore species previously reported in Brazil, for which we do not present any field data are Pseudalopex gymnocercus (pampas fox), Conepatus chinga (Molina's hog-nosed skunk), and Conepatus semistriatus (striped hog-nosed skunk). We report the first tick records in Brazil on two Felidae species (O. colocolo, H. yagouaroundi), two Canidae species (P. vetulus, S. venaticus), one Procyonidae species (P. flavus) and one Mustelidae (E. barbara). Tick infestation remains unreported for 5 of the 26 Carnivora species native in Brazil: Oncifelis geoffroyi (Geoffroy's cat), Atelocynus microtis (short-eared dog), Pteronura brasiliensis (giant otter), Mustela africana (Amazon weasel), and Bassaricyon gabbii (olingo). Our field data comprise 16 tick species represented by the genera Amblyomma (12 species), Ixodes (1 species), Dermacentor (1 species), Rhipicephalus (1 species), and Boophilus (1 species). Additional 5 tick species (3 Amblyomma species and 1 species from each of the genera Ixodes and Ornithodoros) were reported in the literature. The most common ticks on Carnivora hosts were Amblyomma ovale (found on 14 host species), Amblyomma cajennense (10 species), Amblyomma aureolatum (10 species), Amblyomma tigrinum (7 species), Amblyomma parvum (7 species), and Boophilus microplus (7 species).

  17. Seroprevalence of Toxoplasma gondii infection in captive mammals in three zoos in Mexico City, Mexico.

    PubMed

    Alvarado-Esquivel, Cosme; Gayosso-Dominguez, Edgar Arturo; Villena, Isabelle; Dubey, J P

    2013-09-01

    Antibodies to Toxoplasma gondii were determined in 167 mammals in three zoos in Mexico City, Mexico, using the modified agglutination test (MAT). Overall, antibodies to T. gondii were found in 89 (53.3%) of the 167 animals tested. Antibodies were found in 35 of 43 wild Felidae: 2 of 2 bobcats (Lynx rufus); 4 of 4 cougars (Puma concolor); 10 of 13 jaguars (Panthera onca); 5 of 5 leopards (Panthera pardus); 7 of 7 lions (Panthera leo); 2 of 3 tigers (Panthera tigris); 2 of 3 ocelots (Leopardus pardalis); 2 of 2 Sumatran tigers (Panthera tigris sumatrae); lof 2 Jaguarundi (Herpailurus jagouaroundi); but not in 0 of 2 oncillas (Leopardus tigrinus). Such high seroprevalence in wild felids is of public health significance because of the potential of oocyst shedding. Four of 6 New World primates (2 of 2 Geoffroy's spider monkeys [Ateles geoffroyi], 1 of 3 Patas monkeys [Erythrocebus patas], and 1 of 1 white-headed capuchin [Cebus capucinus]) had high MAT titers of 3,200, suggesting recently acquired infection; these animals are highly susceptible to clinical toxoplasmosis. However, none of these animals were ill. Seropositivity to T. gondii was found for the first time in a number of species.

  18. A Comparative Analysis of Genetic Diversity and Structure in Jaguars (Panthera onca), Pumas (Puma concolor), and Ocelots (Leopardus pardalis) in Fragmented Landscapes of a Critical Mesoamerican Linkage Zone.

    PubMed

    Wultsch, Claudia; Waits, Lisette P; Kelly, Marcella J

    2016-01-01

    With increasing anthropogenic impact and landscape change, terrestrial carnivore populations are becoming more fragmented. Thus, it is crucial to genetically monitor wild carnivores and quantify changes in genetic diversity and gene flow in response to these threats. This study combined the use of scat detector dogs and molecular scatology to conduct the first genetic study on wild populations of multiple Neotropical felids coexisting across a fragmented landscape in Belize, Central America. We analyzed data from 14 polymorphic microsatellite loci in 1053 scat samples collected from wild jaguars (Panthera onca), pumas (Puma concolor), and ocelots (Leopardus pardalis). We assessed levels of genetic diversity, defined potential genetic clusters, and examined gene flow for the three target species on a countrywide scale using a combination of individual- and population-based analyses. Wild felids in Belize showed moderate levels of genetic variation, with jaguars having the lowest diversity estimates (HE = 0.57 ± 0.02; AR = 3.36 ± 0.09), followed by pumas (HE = 0.57 ± 0.08; AR = 4.20 ± 0.16), and ocelots (HE = 0.63 ± 0.03; AR = 4.16 ± 0.08). We observed low to moderate levels of genetic differentiation for all three target species, with jaguars showing the lowest degree of genetic subdivision across the country, followed by ocelots and pumas. Although levels of genetic diversity and gene flow were still fairly high, we detected evidence of fine-scale genetic subdivision, indicating that levels of genetic connectivity for wild felids in Belize are likely to decrease if habitat loss and fragmentation continue at the current rate. Our study demonstrates the value of understanding fine-scale patterns of gene flow in multiple co-occurring felid species of conservation concern, which is vital for wildlife movement corridor planning and prioritizing future conservation and management efforts within human-impacted landscapes.

  19. A Comparative Analysis of Genetic Diversity and Structure in Jaguars (Panthera onca), Pumas (Puma concolor), and Ocelots (Leopardus pardalis) in Fragmented Landscapes of a Critical Mesoamerican Linkage Zone

    PubMed Central

    Wultsch, Claudia; Waits, Lisette P.; Kelly, Marcella J.

    2016-01-01

    With increasing anthropogenic impact and landscape change, terrestrial carnivore populations are becoming more fragmented. Thus, it is crucial to genetically monitor wild carnivores and quantify changes in genetic diversity and gene flow in response to these threats. This study combined the use of scat detector dogs and molecular scatology to conduct the first genetic study on wild populations of multiple Neotropical felids coexisting across a fragmented landscape in Belize, Central America. We analyzed data from 14 polymorphic microsatellite loci in 1053 scat samples collected from wild jaguars (Panthera onca), pumas (Puma concolor), and ocelots (Leopardus pardalis). We assessed levels of genetic diversity, defined potential genetic clusters, and examined gene flow for the three target species on a countrywide scale using a combination of individual- and population-based analyses. Wild felids in Belize showed moderate levels of genetic variation, with jaguars having the lowest diversity estimates (HE = 0.57 ± 0.02; AR = 3.36 ± 0.09), followed by pumas (HE = 0.57 ± 0.08; AR = 4.20 ± 0.16), and ocelots (HE = 0.63 ± 0.03; AR = 4.16 ± 0.08). We observed low to moderate levels of genetic differentiation for all three target species, with jaguars showing the lowest degree of genetic subdivision across the country, followed by ocelots and pumas. Although levels of genetic diversity and gene flow were still fairly high, we detected evidence of fine-scale genetic subdivision, indicating that levels of genetic connectivity for wild felids in Belize are likely to decrease if habitat loss and fragmentation continue at the current rate. Our study demonstrates the value of understanding fine-scale patterns of gene flow in multiple co-occurring felid species of conservation concern, which is vital for wildlife movement corridor planning and prioritizing future conservation and management efforts within human-impacted landscapes. PMID:26974968

  20. Image-based red cell counting for wild animals blood.

    PubMed

    Mauricio, Claudio R M; Schneider, Fabio K; Dos Santos, Leonilda Correia

    2010-01-01

    An image-based red blood cell (RBC) automatic counting system is presented for wild animals blood analysis. Images with 2048×1536-pixel resolution acquired on an optical microscope using Neubauer chambers are used to evaluate RBC counting for three animal species (Leopardus pardalis, Cebus apella and Nasua nasua) and the error found using the proposed method is similar to that obtained for inter observer visual counting method, i.e., around 10%. Smaller errors (e.g., 3%) can be obtained in regions with less grid artifacts. These promising results allow the use of the proposed method either as a complete automatic counting tool in laboratories for wild animal's blood analysis or as a first counting stage in a semi-automatic counting tool.

  1. Phylogenetic reconstruction of South American felids defined by protein electrophoresis.

    PubMed

    Slattery, J P; Johnson, W E; Goldman, D; O'Brien, S J

    1994-09-01

    Phylogenetic associations among six closely related South American felid species were defined by changes in protein-encoding gene loci. We analyzed proteins isolated from skin fibroblasts using two-dimensional electrophoresis and allozymes extracted from blood cells. Genotypes were determined for multiple individuals of ocelot, margay, tigrina, Geoffroy's cat, kodkod, and pampas cat at 548 loci resolved by two-dimensional electrophoresis and 44 allozyme loci. Phenograms were constructed using the methods of Fitch-Margoliash and neighbor-joining on a matrix of Nei's unbiased genetic distances for all pairs of species. Results of a relative-rate test indicate changes in two-dimensional electrophoresis data are constant among all South American felids with respect to a hyena outgroup. Allelic frequencies were transformed to discrete character states for maximum parsimony analysis. Phylogenetic reconstruction indicates a major split occurred approximately 5-6 million years ago, leading to three groups within the ocelot lineage. The earliest divergence led to Leopardus tigrina, followed by a split between an ancestor of an unresolved trichotomy of three species (Oncifelis guigna, O. geoffroyi, and Lynchailuris colocolo) and a recent common ancestor of Leopardus pardalis and L. wiedii. The results suggest that modern South American felids are monophyletic and evolved rapidly after the formation of the Panama land bridge between North and South America.

  2. Cross transmission of gastrointestinal nematodes between captive neotropical felids and feral cats.

    PubMed

    Rendón-Franco, Emilio; Romero-Callejas, Evangelina; Villanueva-García, Claudia; Osorio-Sarabia, David; Muñoz-García, Claudia I

    2013-12-01

    Pathogen cross transmission between wildlife and domestic animals represents an extinction risk for wildlife; however, reliable verification is difficult to perform, and in some cases, it is even considered unlikely to be conducted. The aim of this work was to identify cross transmission of helminths between feral cats and captive wild felids at a zoological park in southeastern Mexico. Feces were collected from jaguars (Panthera onca), cougars (Puma concolor), ocelots (Leopardus pardalis), margays (Leopardus wiedii), and jaguarundis (Puma yagouaroundi). A flotation technique and macroscopic sieving were performed on the feces. Additionally, as part of the noxious fauna control program of the park, feral cats were captured and euthanized. To perform parasitologic studies, helminths from these animals were recovered. Toxocara cati and Trichuris campanula were shared by jaguarundis and feral cats. Ancylostoma sp. was found in jaguar and ocelot and Ancylostoma tubaeforme in feral cats. Additionally, during this study, a couple of jaguarundis died with clinical signs of trichuriasis. This is the first report of T. campanula in jaguarundi. Because feral cats roam freely in the park, transmission could occur from these vertebrates to wild felids. This study shows the risk that parasites represent to wild felids; a similar situation could be found in free-living species, especially in fragmented habitats that favor contact with domestic animals.

  3. Exposure of Free-Ranging Wild Carnivores and Domestic Dogs to Canine Distemper Virus and Parvovirus in the Cerrado of Central Brazil.

    PubMed

    Furtado, Mariana Malzoni; Hayashi, Erika Midori Kida; Allendorf, Susan Dora; Coelho, Claudio José; de Almeida Jácomo, Anah Tereza; Megid, Jane; Ramos Filho, José Domingues; Silveira, Leandro; Tôrres, Natália Mundim; Ferreira Neto, José Soares

    2016-09-01

    Human population growth around protected areas increases the contact between wild and domestic animals, promoting disease transmission between them. This study investigates the exposure of free-ranging wild carnivores and domestic dogs to canine distemper virus (CDV) and parvovirus in Emas National Park (ENP) in the Cerrado savanna of central Brazil. Serum samples were collected from 169 wild carnivores, including the maned wolf (Chrysocyon brachyurus), crab-eating fox (Cerdocyon thous), hoary fox (Pseudalopex vetulus), puma (Puma concolor), ocelot (Leopardus pardalis), pampas cat (Leopardus colocolo), jaguarundi (Herpailurus yagouaroundi), striped hog-nosed skunk (Conepatus semistriatus) and coati (Nasua nasua), and from 35 domestic dogs living on rural properties bordering ENP. Serological tests showed that 10.6% of wild carnivores (maned wolves, crab-eating foxes and ocelots) and 71.4% of domestic dogs were exposed to CDV, and 56.8% of wild carnivores, including all species sampled except coatis, and 57.1% of domestic dogs were exposed to parvovirus. This report is the first to indicate that the free-ranging pampas cat, jaguarundi and striped hog-nosed skunk are exposed to parvovirus. CDV and parvovirus deserve attention in ENP, and it is extremely important to monitor the health of carnivore populations and perform molecular diagnosis of the viruses to determine the possible involvement of the domestic dog in their transmission.

  4. Disseminated coccidioidomycosis in a captive Indochinese tiger (Panthera tigris corbetti) with chronic renal disease.

    PubMed

    Helmick, Kelly E; Koplos, Peter; Raymond, James

    2006-12-01

    A 19-yr-old, 78.2-kg captive female Indochinese tiger (Panthera tigris corbetti) from the El Paso Zoo (El Paso, Texas, USA) with chronic renal disease was euthanized after a 10-day course of anorexia, depression, progressive rear limb weakness, muscle fasciculations, and head tremors. Postmortem findings included pericardial effusion, generalized lymphadenopathy, glomerulosclerosis, glomerular atrophy with membranous glomerulonephropathy, and pancreatic adenocarcinoma. Pyogranulomatous pneumonia, pericarditis, and lymphadenitis were associated with fungal spherules histomorphologically consistent with Coccidioides immitis. Rising antibodies to C. immitis were detected on samples obtained perimortem and 2 mo before euthanasia. Retrospective serology was negative for two additional Indochinese tigers, two Iranian leopards (Panthera pardus saxicolor), two jaguars (Panthera onca), two bobcats (Lynx rufus texensis), two ocelots (Leopardus pardalis), and three Amur leopards (Panthera pardus orientalis) housed at the zoo over an 8-yr period. Despite being located within the endemic region for C. immitis, this is only the second case of coccidioidomycosis reported from this institution.

  5. Molecular identification of Spirometra spp. (Cestoda: Diphyllobothriidae) in some wild animals from Brazil.

    PubMed

    Almeida, Gregório Guilherme; Coscarelli, Daniel; Melo, Maria Norma; Melo, Alan Lane; Pinto, Hudson Alves

    2016-10-01

    Species of the genus Spirometra are diphyllobothriid tapeworms with complex life cycles and are involved in human sparganosis, a neglected disease that affects individuals worldwide. Although some species were reported in wild felids and human cases of sparganosis were described in Brazil, the biology and taxonomy of these parasites are poorly understood. In the present study, samples of diphyllobothriids (eggs and/or proglottids) obtained from the stools of wild carnivores (Leopardus pardalis and Lycalopex vetulus) and plerocercoid larvae found in a snake (Crotalus durissus) from Brazil were analysed by amplifying a fragment of the gene cytochrome c oxidase subunit 1 (cox 1). The DNA sequences obtained here for the first time from the Spirometra spp. from Brazil were used to evaluate the phylogenetic relationships with other species. Molecular data identified two species in the Brazilian samples (evolutionary divergence of 17.8-19.2%). The species were identified as Spirometra sp. 1, found in Le. pardalis, and Spirometra sp. 2 found in Ly. vetulus and C. durissus, and they differed from Asian isolates of Spirometra erinaceieuropaei (17.5-20.2% and 12.2-15.6%, respectively), a species previously considered to be distributed worldwide. Moreover, Spirometra sp. 1 is genetically distinct from Sparganum proliferum from Venezuela (19.6-20.4%), while Spirometra sp. 2 is more closely related with the Venezuelan species (6.1-7.0%). Sequences of Spirometra sp. 2 revealed that it is conspecific with the Argentinean isolate of Spirometra found in Lycalopex gymnocercus (1.9-2.2%). Taxonomic and phylogenetic aspects related to New World species of Spirometra are briefly discussed. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  6. Ocelots on Barro Colorado Island are infected with feline immunodeficiency virus but not other common feline and canine viruses.

    PubMed

    Franklin, Samuel P; Kays, Roland W; Moreno, Ricardo; TerWee, Julie A; Troyer, Jennifer L; VandeWoude, Sue

    2008-07-01

    Transmission of pathogens from domestic animals to wildlife populations (spill-over) has precipitated local wildlife extinctions in multiple geographic locations. Identifying such events before they cause population declines requires differentiating spillover from endemic disease, a challenge complicated by a lack of baseline data from wildlife populations that are isolated from domestic animals. We tested sera collected from 12 ocelots (Leopardus pardalis) native to Barro Colorado Island, Panama, which is free of domestic animals, for antibodies to feline herpes virus, feline calicivirus, feline corona virus, feline panleukopenia virus, canine distemper virus, and feline immunodeficiency virus (FIV), typically a species-specific infection. Samples also were tested for feline leukemia virus antigens. Positive tests results were only observed for FIV; 50% of the ocelots were positive. We hypothesize that isolation of this population has prevented introduction of pathogens typically attributed to contact with domestic animals. The high density of ocelots on Barro Colorado Island may contribute to a high prevalence of FIV infection, as would be expected with increased contact rates among conspecifics in a geographically restricted population.

  7. Ocelots on Barro Colorado Island Are Infected with Feline Immunodeficiency Virus but Not Other Common Feline and Canine Viruses

    PubMed Central

    Franklin, Samuel P.; Kays, Roland W.; Moreno, Ricardo; TerWee, Julie A.; Troyer, Jennifer L.; VandeWoude, Sue

    2011-01-01

    Transmission of pathogens from domestic animals to wildlife populations (spill-over) has precipitated local wildlife extinctions in multiple geographic locations. Identifying such events before they cause population declines requires differentiating spillover from endemic disease, a challenge complicated by a lack of baseline data from wildlife populations that are isolated from domestic animals. We tested sera collected from 12 ocelots (Leopardus pardalis) native to Barro Colorado Island, Panama, which is free of domestic animals, for antibodies to feline herpes virus, feline calicivirus, feline corona virus, feline panleukopenia virus, canine distemper virus, and feline immunodeficiency virus (FIV), typically a species-specific infection. Samples also were tested for feline leukemia virus antigens. Positive tests results were only observed for FIV; 50% of the ocelots were positive. We hypothesize that isolation of this population has prevented introduction of pathogens typically attributed to contact with domestic animals. The high density of ocelots on Barro Colorado Island may contribute to a high prevalence of FIV infection, as would be expected with increased contact rates among conspecifics in a geographically restricted population. PMID:18689668

  8. Ocelot Population Status in Protected Brazilian Atlantic Forest.

    PubMed

    Massara, Rodrigo Lima; Paschoal, Ana Maria de Oliveira; Doherty, Paul Francis; Hirsch, André; Chiarello, Adriano Garcia

    2015-01-01

    Forest fragmentation and habitat loss are detrimental to top carnivores, such as jaguars (Panthera onca) and pumas (Puma concolor), but effects on mesocarnivores, such as ocelots (Leopardus pardalis), are less clear. Ocelots need native forests, but also might benefit from the local extirpation of larger cats such as pumas and jaguars through mesopredator release. We used a standardized camera trap protocol to assess ocelot populations in six protected areas of the Atlantic forest in southeastern Brazil where over 80% of forest remnants are < 50 ha. We tested whether variation in ocelot abundance could be explained by reserve size, forest cover, number of free-ranging domestic dogs and presence of top predators. Ocelot abundance was positively correlated with reserve size and the presence of top predators (jaguar and pumas) and negatively correlated with the number of dogs. We also found higher detection probabilities in less forested areas as compared to larger, intact forests. We suspect that smaller home ranges and higher movement rates in smaller, more degraded areas increased detection. Our data do not support the hypothesis of mesopredator release. Rather, our findings indicate that ocelots respond negatively to habitat loss, and thrive in large protected areas inhabited by top predators.

  9. Ocelot Population Status in Protected Brazilian Atlantic Forest

    PubMed Central

    Massara, Rodrigo Lima; Paschoal, Ana Maria de Oliveira; Doherty, Paul Francis; Hirsch, André; Chiarello, Adriano Garcia

    2015-01-01

    Forest fragmentation and habitat loss are detrimental to top carnivores, such as jaguars (Panthera onca) and pumas (Puma concolor), but effects on mesocarnivores, such as ocelots (Leopardus pardalis), are less clear. Ocelots need native forests, but also might benefit from the local extirpation of larger cats such as pumas and jaguars through mesopredator release. We used a standardized camera trap protocol to assess ocelot populations in six protected areas of the Atlantic forest in southeastern Brazil where over 80% of forest remnants are < 50 ha. We tested whether variation in ocelot abundance could be explained by reserve size, forest cover, number of free-ranging domestic dogs and presence of top predators. Ocelot abundance was positively correlated with reserve size and the presence of top predators (jaguar and pumas) and negatively correlated with the number of dogs. We also found higher detection probabilities in less forested areas as compared to larger, intact forests. We suspect that smaller home ranges and higher movement rates in smaller, more degraded areas increased detection. Our data do not support the hypothesis of mesopredator release. Rather, our findings indicate that ocelots respond negatively to habitat loss, and thrive in large protected areas inhabited by top predators. PMID:26560347

  10. [Population estimates and conservation of felids (Carnivora: Felidae) in Northern Quintana Roo, Mexico].

    PubMed

    Ávila-Nájera, Dulce María; Chávez, Cuauhtémoc; Lazcano-Barrero, Marco A; Pérez-Elizalde, Sergio; Alcántara-Carbajal, José Luis

    2015-09-01

    Wildlife density estimates provide an idea of the current state of populations, and in some cases, reflect the conservation status of ecosystems, essential aspects for effective management actions. In Mexico, several regions have been identified as high priority areas for the conservation of species that have some level of risk, like the Yucatan Peninsula (YP), where the country has the largest population of jaguars. However, little is known about the current status of threatened and endangered felids, which coexist in the Northeastern portion of the Peninsula. Our objective was to estimate the wild cats' density population over time at El Eden Ecological Reserve (EEER) and its surrounding areas. Camera trap surveys over four years (2008, 2010, 2011 and 2012) were conducted, and data were obtained with the use of capture-recapture models for closed populations (CAPTURE + MMDM or 1/2 MMDM), and the spatially explicit capture-recapture model (SPACECAP). The species studied were jaguar (Panthera onca), puma (Puma concolor), ocelot (Leopardus pardalis), jaguarundi (Puma yaguaroundi) and margay (Leopardus wiedii). Capture frequency was obtained for all five species and the density for three (individuals/100km2). The density estimated with The Mean Maximum Distance Moved (MMDM), CAPTURE, ranged from 1.2 to 2.6 for jaguars, from 1.7 to 4.3 for pumas and from 1.4 to 13.8 for ocelots. The density estimates in SPACECAP ranged from 0.7 to 3.6 for jaguars, from 1.8 to 5.2 for pumas and 2.1 to 5.1 for ocelots. Spatially explicit capture recapture (SECR) methods in SPACECAP were less likely to overestimate densities, making it a useful tool in the planning and decision making process for the conservation of these species. The Northeastern portion of the Yucatan Peninsula maintains high populations of cats, the EEER and its surrounding areas are valuable sites for the conservation of this group of predators. Rev. Biol.

  11. Computed tomography imaging of a leopard tortoise (Geochelone pardalis pardalis) with confirmed pulmonary fibrosis: a case report

    PubMed Central

    2013-01-01

    An approximately 20-year-old, female Leopard tortoise (Geochelone pardalis pardalis) was presented with dypsnea, wheezing, anorexia and depression. Whole body radiographs revealed generalized diffuse unstructured ‘interstitial lung pattern’ with thickened pulmonary septae while computed tomography (CT) showed emphysematous lung parenchyma and thickened pulmonary septae bordered by irregular ground-glass opacity with smaller areas of ‘honeycombing’. These imaging findings together with histopathologic findings were compatible with chronic, extensive ‘interstitial’ pulmonary fibrosis. PMID:23618386

  12. Resistance to Antibiotics of Clinical Relevance in the Fecal Microbiota of Mexican Wildlife

    PubMed Central

    Cristóbal-Azkarate, Jurgi; Dunn, Jacob C.; Day, Jennifer M. W.; Amábile-Cuevas, Carlos F.

    2014-01-01

    There are a growing number of reports of antibiotic resistance (ATBR) in bacteria living in wildlife. This is a cause for concern as ATBR in wildlife represents a potential public health threat. However, little is known about the factors that might determine the presence, abundance and dispersion of ATBR bacteria in wildlife. Here, we used culture and molecular methods to assess ATBR in bacteria in fecal samples from howler monkeys (Alouatta palliata), spider monkeys (Ateles geoffroyi), tapirs (Tapirus bairdii) and felids (jaguars, Panthera onca; pumas, Puma concolor; jaguarundis, Puma yagouaroundi; and ocelots, Leopardus pardalis) living freely in two regions of the Mexican state of Veracruz under different degrees of human influence. Overall, our study shows that ATBR is commonplace in bacteria isolated from wildlife in southeast Mexico. Most of the resistances were towards old and naturally occurring antibiotics, but we also observed resistances of potential clinical significance. We found that proximity to humans positively affected the presence of ATBR and that ATBR was higher in terrestrial than arboreal species. We also found evidence suggesting different terrestrial and aerial routes for the transmission of ATBR between humans and wildlife. The prevalence and potential ATBR transfer mechanisms between humans and wildlife observed in this study highlight the need for further studies to identify the factors that might determine ATBR presence, abundance and distribution. PMID:25233089

  13. Conservation value of a native forest fragment in a region of extensive agriculture.

    PubMed

    Chiarello

    2000-05-01

    A survey of mammals and birds was carried out in a semi-deciduous forest fragment of 150 ha located in a zone of intensive agriculture in Ribeirão Preto, State of São Paulo, south-eastern Brazil. Line transect sampling was used to census mammals and birds during six days, totalling 27.8 km of trails and 27.8 hours of observation. Twenty mammal species were confirmed in the area (except bats and small mammals), including rare or endangered species, such as the mountain lion (Puma concolor), the maned wolf (Chrysocyon brachyurus), and the ocelot (Leopardus pardalis). The brown capuchin monkey (Cebus apella) and the black-tufted-ear marmoset (Callithrix penicillata) were found frequently, suggesting high population density in the fragment. Regarding the avifauna, 49 bird species were recorded, most of them typical of open areas or forest edges. Some confirmed species, however, are becoming increasingly rare in the region, as for example the muscovy duck (Cairina moschata) and the toco toucan (Ramphastos toco). The results demonstrate that forest fragment of this size are refuges for native fauna in a region dominated almost exclusively by sugar-cane plantations. Besides faunal aspects, the conservation of these fragments is of great importance for the establishment of studies related to species preservation in the long term, including reintroduction and translocation projects, as well as studies related to genetic health of isolated populations.

  14. Using niche-modelling and species-specific cost analyses to determine a multispecies corridor in a fragmented landscape

    PubMed Central

    Zurano, Juan Pablo; Selleski, Nicole; Schneider, Rosio G.

    2017-01-01

    Misiones, Argentina, contains the largest remaining tract of Upper Paraná Atlantic Forest ecoregion; however, ~50% of native forest is unprotected and located in a mosaic of plantations, agriculture, and pastures. Existing protected areas are becoming increasingly isolated due to ongoing habitat modification. These factors, combined with lower than expected regional carnivore densities, emphasize the need to understand the effect of fragmentation on animal movement and connectivity between protected areas. Using detection dogs and genetic analyses of scat, we collected data on jaguars (Panthera onca), pumas (Puma concolor), ocelots (Leopardus pardalis), oncillas (Leopardus tigrinus), and bush dogs (Speothos venaticus) across habitats that varied in vegetation, disturbance, human proximity, and protective status. With MaxEnt we evaluated habitat use, habitat suitability, and potential species richness for the five carnivores across northern-central Misiones, Argentina. Through a multifaceted cost analysis that included unique requirements of each carnivore and varying degrees of overlap among them, we determined the optimal location for primary/secondary corridors that would link the northern-central zones of the Green Corridor in Misiones and identified areas within these corridors needing priority management. A secondary analysis, comparing these multispecies corridors with the jaguar’s unique requirements, demonstrated that this multispecies approach balanced the preferences of all five species and effectively captured areas required by this highly restricted and endangered carnivore. We emphasize the potential importance of expanding beyond a single umbrella or focal species when developing biological corridors that aim to capture the varied ecological requirements of coexisting species and ecological processes across the landscape. Detection dogs and genetic analyses of scat allow data on multiple species to be collected efficiently across multiple habitat types independent of the degree of legal protection. These data used with multifocal GIS analyses balance the varying degree of overlap and unique properties among them allowing for comprehensive conservation strategies to be developed relatively rapidly. Our comprehensive approach serves as a model to other regions faced with habitat loss and lack of data. The five carnivores focused on in our study have wide ranges, so the results from this study can be expanded and combined with surrounding countries, with analyses at the species or community level. PMID:28841692

  15. Using niche-modelling and species-specific cost analyses to determine a multispecies corridor in a fragmented landscape.

    PubMed

    DeMatteo, Karen E; Rinas, Miguel A; Zurano, Juan Pablo; Selleski, Nicole; Schneider, Rosio G; Argüelles, Carina F

    2017-01-01

    Misiones, Argentina, contains the largest remaining tract of Upper Paraná Atlantic Forest ecoregion; however, ~50% of native forest is unprotected and located in a mosaic of plantations, agriculture, and pastures. Existing protected areas are becoming increasingly isolated due to ongoing habitat modification. These factors, combined with lower than expected regional carnivore densities, emphasize the need to understand the effect of fragmentation on animal movement and connectivity between protected areas. Using detection dogs and genetic analyses of scat, we collected data on jaguars (Panthera onca), pumas (Puma concolor), ocelots (Leopardus pardalis), oncillas (Leopardus tigrinus), and bush dogs (Speothos venaticus) across habitats that varied in vegetation, disturbance, human proximity, and protective status. With MaxEnt we evaluated habitat use, habitat suitability, and potential species richness for the five carnivores across northern-central Misiones, Argentina. Through a multifaceted cost analysis that included unique requirements of each carnivore and varying degrees of overlap among them, we determined the optimal location for primary/secondary corridors that would link the northern-central zones of the Green Corridor in Misiones and identified areas within these corridors needing priority management. A secondary analysis, comparing these multispecies corridors with the jaguar's unique requirements, demonstrated that this multispecies approach balanced the preferences of all five species and effectively captured areas required by this highly restricted and endangered carnivore. We emphasize the potential importance of expanding beyond a single umbrella or focal species when developing biological corridors that aim to capture the varied ecological requirements of coexisting species and ecological processes across the landscape. Detection dogs and genetic analyses of scat allow data on multiple species to be collected efficiently across multiple habitat types independent of the degree of legal protection. These data used with multifocal GIS analyses balance the varying degree of overlap and unique properties among them allowing for comprehensive conservation strategies to be developed relatively rapidly. Our comprehensive approach serves as a model to other regions faced with habitat loss and lack of data. The five carnivores focused on in our study have wide ranges, so the results from this study can be expanded and combined with surrounding countries, with analyses at the species or community level.

  16. Reproductive periodicity and steroid hormone profiles in the sex-changing coral-reef fish, Plectropomus leopardus

    NASA Astrophysics Data System (ADS)

    Frisch, A. J.; McCormick, M. I.; Pankhurst, N. W.

    2007-03-01

    The reproductive biology of coral trout, Plectropomus leopardus, from the Great Barrier Reef (Australia) was investigated by correlating gonadal condition with plasma levels of gonadal steroids. Female fish were found to be regressed from mid-summer to early spring, after which rapid and cyclical increases in gonado-somatic index ( I G), maximum oocyte diameter (MOD) and plasma concentrations of estradiol-17β and testosterone were detected. Male fish, in contrast, commenced recrudescence slightly earlier in winter and responded with less dramatic increases in both I G and plasma concentrations of testosterone and 11-ketotestosterone. The mode of oocyte development was multiple group-synchronous, and cyclical fluctuations in reproductive parameters ( I G, MOD and gonadal steroid concentrations) were synchronized with new-moon lunar phases. It is likely, therefore, that individual P. leopardus have the capacity to spawn on multiple occasions, with lunar periodicity. However, evidence suggests that early bouts of reproduction may be more important in terms of reproductive investment than subsequent bouts later in the same season. It is concluded that patterns of gametogenesis and steroidogenesis in P. leopardus are similar to the patterns displayed by other tropical groupers, suggesting that management regimes and propagation protocols developed for these fishes may also be appropriate for use with P. leopardus.

  17. Increased accuracy of batch fecundity estimates using oocyte stage ratios in Plectropomus leopardus.

    PubMed

    Carter, A B; Williams, A J; Russ, G R

    2009-08-01

    Using the ratio of the number of migratory nuclei to hydrated oocytes to estimate batch fecundity of common coral trout Plectropomus leopardus increases the time over which samples can be collected and, therefore, increases the sample size available and reduces biases in batch fecundity estimates.

  18. Characterization of dermal plates from armored catfish Pterygoplichthys pardalis reveals sandwich-like nanocomposite structure.

    PubMed

    Ebenstein, Donna; Calderon, Carlos; Troncoso, Omar P; Torres, Fernando G

    2015-05-01

    Dermal plates from armored catfish are bony structures that cover their body. In this paper we characterized structural, chemical, and nanomechanical properties of the dermal plates from the Amazonian fish Pterygoplichthys pardalis. Analysis of the morphology of the plates using scanning electron microscopy (SEM) revealed that the dermal plates have a sandwich-like structure composed of an inner porous matrix surrounded by two external dense layers. This is different from the plywood-like laminated structure of elasmoid fish scales but similar to the structure of osteoderms found in the dermal armour of some reptiles and mammals. Chemical analysis performed using Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC) and X-ray diffraction (XRD) results revealed similarities between the composition of P. pardalis plates and the elasmoid fish scales of Arapaima gigas. Reduced moduli of P. pardalis plates measured using nanoindentation were also consistent with reported values for A. gigas scales, but further revealed that the dermal plate is an anisotropic and heterogeneous material, similar to many other fish scales and osteoderms. It is postulated that the sandwich-like structure of the dermal plates provides a lightweight and tough protective layer. Copyright © 2015 Elsevier Ltd. All rights reserved.

  19. Comparative anatomy of the cardiac foramen ovale in cats (Felidae), dogs (Canidae), bears (Ursidae) and hyaenas (Hyaenidae).

    PubMed Central

    Macdonald, A A; Johnstone, M

    1995-01-01

    The structure of the foramen ovale from 16 species representing 4 carnivore families, the Felidae, Canidae, Ursidae and Hyaenidae, was studied using the scanning electron microscope. The Felidae were represented by 9 domestic cat fetuses (Felis catus), 2 snow leopard neonates (Uncia uncia), an ocelot neonate (Leopardus pardalis), 2 lion neonates (Panthera leo), a panther neonate (Panthera pardus) and 3 tigers (Neofelis tigris), comprising 2 fetuses and a neonate. The Canidae were represented by a golden jackal neonate (Canis aureus), a newborn wolf (Canis lupus), 8 domestic dog fetuses (Canis familiaris), 3 red fox neonates (Vulpes vulpes) and a dhole neonate (Cuon alpinus). The Ursidae were represented by a brown bear neonate (Ursus arctos), a day-old grizzly bear cub (Ursus arctos horribilis), a polar bear neonate (Ursus maritimus), and 2 additional bear fetuses (species unknown). The Hyaenidae were represented by a striped hyaena neonate (Hyaena hyaena). In each species, the foramen ovale, when viewed from the terminal part of the caudal vena cava, had the appearance of a short tunnel. A thin fold of tissue, the developed remains of the embryonic septum primum, extended from the distal end of the caudal vena cava for a variable distance into the lumen of the left atrium and contributed towards the 'tunnel' appearance in all specimens. It constituted a large proportion of the tube, and its distal end was straight-edged. There was fibrous material underlying the endothelium of the flap, the apparent morphology of which suggested that it comprised cardiac muscle.(ABSTRACT TRUNCATED AT 250 WORDS) Images Fig. 2 Fig. 3 Fig. 4 PMID:7649822

  20. Use of Atlantic Forest protected areas by free-ranging dogs: estimating abundance and persistence of use

    USGS Publications Warehouse

    Paschoal, Ana Maria; Massara, Rodrigo; Bailey, Larissa L.; Kendall, William L.; Doherty, Paul F.; Hirsch, Andre; Chiarello, Adriano; Paglia, Adriano

    2016-01-01

    Worldwide, domestic dogs (Canis familiaris) are one of the most common carnivoran species in natural areas and their populations are still increasing. Dogs have been shown to impact wildlife populations negatively, and their occurrence can alter the abundance, behavior, and activity patterns of native species. However, little is known about abundance and density of the free-ranging dogs that use protected areas. Here, we used camera trap data with an open-robust design mark–recapture model to estimate the number of dogs that used protected areas in Brazilian Atlantic Forest. We estimated the time period these dogs used the protected areas, and explored factors that influenced the probability of continued use (e.g., season, mammal richness, proportion of forest), while accounting for variation in detection probability. Dogs in the studied system were categorized as rural free-ranging, and their abundance varied widely across protected areas (0–73 individuals). Dogs used protected areas near human houses for longer periods (e.g., >50% of sampling occasions) compared to more distant areas. We found no evidence that their probability of continued use varied with season or mammal richness. Dog detection probability decreased linearly among occasions, possibly due to the owners confining their dogs after becoming aware of our presence. Comparing our estimates to those for native carnivoran, we found that dogs were three to 85 times more abundant than ocelots (Leopardus pardalis), two to 25 times more abundant than puma (Puma concolor), and approximately five times more abundant than the crab-eating fox (Cerdocyon thous). Combining camera trapping data with modern mark–recapture methods provides important demographic information on free-ranging dogs that can guide management strategies to directly control dogs' abundance and ranging behavior.

  1. Echinococcus oligarthrus in the subtropical region of Argentina: First integration of morphological and molecular analyses determines two distinct populations.

    PubMed

    Arrabal, Juan Pablo; Avila, Hector Gabriel; Rivero, Maria Romina; Camicia, Federico; Salas, Martin Miguel; Costa, Sebastián A; Nocera, Carlos G; Rosenzvit, Mara C; Kamenetzky, Laura

    2017-06-15

    Echinococcosis is a parasitic zoonosis that is considered as a neglected disease by the World Health Organization. The species Echinococcus oligarthrus is one of the causative agents of Neotropical echinococcosis, which is a poorly understood disease that requires a complex medical examination, may threaten human life, and is frequently associated with a low socioeconomic status. Morphological and genetic diversity in E. oligarthrus remains unknown. The aim of this work is to identify and characterize E. oligarthrus infections in sylvatic animals from the Upper Paraná Atlantic Forest in the province of Misiones, Argentina, by following an integrative approach that links morphological, genetic and ecological aspects. This study demonstrates, for the first time, one of the complete life cycles of E. oligarthrus in an important ecoregion. The Upper Paraná Atlantic Forest constitutes the largest remnant continuous forest of the Atlantic Forest, representing 7% of the world's biodiversity. This is the first molecular determination of E. oligarthrus in Argentina. In addition, the agouti (Dasyprocta azarae), the ocelot (Leopardus pardalis) and the puma (Puma concolor) were identified as sylvatic hosts of Neotropical echinococcosis caused by E. oligarthrus. Mitochondrial and nuclear molecular marker analyses showed a high genetic diversity in E. oligarthrus. Moreover, the genetic distance found among E. oligarthrus isolates is higher than the one observed among Echinococcus granulosus genotypes, which clearly indicates that there are at least two different E. oligarthrus populations in Argentina. This study provides valuable information to understand the underlying conditions that favour the maintenance of E. oligarthrus in sylvatic cycles and to evaluate its zoonotic significance for devising preventive measures for human and animal wellbeing. Copyright © 2017 Elsevier B.V. All rights reserved.

  2. Spatial variation in the effects of size and age on reproductive dynamics of common coral trout Plectropomus leopardus.

    PubMed

    Carter, A B; Russ, G R; Tobin, A J; Williams, A J; Davies, C R; Mapstone, B D

    2014-04-01

    The effects of size and age on reproductive dynamics of common coral trout Plectropomus leopardus populations were compared between coral reefs open or closed (no-take marine reserves) to fishing and among four geographic regions of the Great Barrier Reef (GBR), Australia. The specific reproductive metrics investigated were the sex ratio, the proportion of vitellogenic females and the spawning fraction of local populations. Sex ratios became increasingly male biased with length and age, as expected for a protogyne, but were more male biased in southern regions of the GBR (Mackay and Storm Cay) than in northern regions (Lizard Island and Townsville) across all lengths and ages. The proportion of vitellogenic females also increased with length and age. Female P. leopardus were capable of daily spawning during the spawning season, but on average spawned every 4·3 days. Mature females spawned most frequently on Townsville reserve reefs (every 2·3 days) and Lizard Island fished reefs (every 3·2 days). Females on Mackay reefs open to fishing showed no evidence of spawning over 4 years of sampling, while females on reserve reefs spawned only once every 2-3 months. No effect of length on spawning frequency was detected. Spawning frequency increased with age on Lizard Island fished reefs, declined with age on Storm Cay fished reefs, and declined with age on reserve reefs in all regions. It is hypothesized that the variation in P. leopardus sex ratios and spawning frequency among GBR regions is primarily driven by water temperature, while no-take management zones influence spawning frequency depending on the region in which the reserve is located. Male bias and lack of spawning activity on southern GBR, where densities of adult P. leopardus are highest, suggest that recruits may be supplied from central or northern GBR. Significant regional variation in reproductive traits suggests that a regional approach to management of P. leopardus is appropriate and highlights the need for considering spatial variation in reproduction where reserves are used as fishery or conservation management tools. © 2014 The Fisheries Society of the British Isles.

  3. Detection of testudinid herpesvirus type 4 in a leopard tortoise (Stigmochelys pardalis).

    PubMed

    Kolesnik, Ekaterina; Mittenzwei, Frank; Marschang, Rachel E

    2016-08-17

    Several animals from a mixed species collection of tortoises in Germany died unexpectedly. Some of the affected leopard tortoises (Stigmochelys pardalis) from this group showed respiratory signs. Samples were collected from one of the ill tortoises, and a Mycoplasma spp. and a herpesvirus were detected by PCR. Sequencing of a portion of the DNA polymerase gene of the herpesvirus showed 99% identity with testudinid herpesvirus 4, previously described only once in a bowsprit tortoise (Chersina angulata) in the United States.

  4. Medium- and large-sized mammals in a steppic savanna area of the Brazilian Pampa: survey and conservation issues of a poorly known fauna.

    PubMed

    Espinosa, C C; Galiano, D; Kubiak, B B; Marinho, J R

    2016-02-01

    The wildlife of the Brazilian Pampa is threatened by large-scale habitat loss, due in particular to the expansion of soybean cultivation and the conversion of grasslands areas into extensive areas of silviculture. It is essential to study how the mammal fauna copes with the highly fragmented, human-influenced, non-protected landscape. Our study presents the results of a survey of the large- and medium-sized mammals of a typical human-influenced steppic savanna area of the Pampa biome. The survey was conducted exclusively with the use of camera traps over a period of 16 months. The relative frequencies of species in the area were evaluated. We recorded 18 species, some of them locally threatened (Tamandua tetradactyla, Alouatta caraya, Leopardus colocolo, Leopardus geoffroyi, Leopardus wiedii, Puma yagouaroundi, Mazama gouazoubira and Cuniculus paca). Several species were found to thrive in the area; however, many species were considered rare, and undoubtedly new species could be recorded if we continued the sampling. Our results contribute to the knowledge of faunal diversity in the Pampa biome and associated habitats, warn about threats and provide support for conservation measures.

  5. Exposure to silver nanoparticles produces oxidative stress and affects macromolecular and metabolic biomarkers in the goodeid fish Chapalichthys pardalis.

    PubMed

    Valerio-García, Roberto Carlos; Carbajal-Hernández, Ana Laura; Martínez-Ruíz, Erika Berenice; Jarquín-Díaz, Víctor Hugo; Haro-Pérez, Catalina; Martínez-Jerónimo, Fernando

    2017-04-01

    Silver nanoparticles (AgNPs) are the most commercialized nanomaterial worldwide, mainly due to their microbicidal activity. Although, AgNPs have been shown to be toxic to aquatic species, their effect on endemic fish, like Goodeidae, has not been demonstrated. Endemic species are under strong pressures by anthropogenic contamination and destruction of their habitat; therefore, we studied adult Chapalichthys pardalis, an endemic fish of Mexico. We evaluated the toxic effect of AgNPs through oxidative stress, macromolecular and metabolic biomarkers. We determined the LC 50 (96h) and performed subchronic tests (21days) using sublethal AgNPs concentrations (equivalent to CL 1 and CL 10 ). At the end of the bioassay, we quantified 10 stress biomarkers in the liver, gills, and muscle, including the antioxidant enzymes (superoxide dismutase [SOD], catalase [CAT], and glutathione [GPx]), thiobarbituric acid reactive species (TBARS), protein oxidation (CO), macromolecules (proteins, lipids, and carbohydrates), and metabolites (glucose and lactate). In addition, we determined the integrated biomarkers response (IBR). LC 50 was of 10.32mgL -1 . Results of subchronic exposure (21days) revealed that AgNPs produce oxidative stress in C. pardalis adults, as evidenced by a diminution in antioxidant enzymes activity and an increase in TBARS and oxidized proteins. AgNPs also diminished levels of macromolecules and generated a high-energy consumption, reflected in the reduction of glucose levels, although lactate levels were not altered. The IBR analysis evidenced that the largest effect was produced in organisms exposed to LC 10 , being the liver and gills the organs with the greatest damage. Results demonstrated that exposure to AgNPs induces acute and chronic toxic effects on C. pardalis and forewarns about the impact that these nanomaterials can exert on these ecologically relevant aquatic organisms. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. [Diversity, relative abundance and activity patterns of medium and large mammals in a tropical deciduous forest in the Isthmus of Tehuantepec, Oaxaca, Mexico].

    PubMed

    Cortés-Marcial, Malinalli; Briones-Salas, Miguel

    2014-12-01

    The use of camera traps and mammal track search are complementary methods to monitoring species of which is not well documented their natural history, as in the case of medium and large mammals. To ensure its conservation and good management, it is necessary to generate information about the structure of the community and their populations. The objective of the present study was to estimate the diversity, relative abundance and activity patterns of medium and large mammals in a tropical deciduous forest located in the Isthmus of Tehuantepec, Oaxaca, Mexico. Samplings were conducted in three month intervals, from September 2011 to May 2013. We used photographic-sampling and track search, two complementary sampling methods. For photographic-sampling, 12 camera traps were placed covering an area of 60 km2, while for the tracks search a monthly tour of four line-transect surveys of three kilometers length each was undertaken. We obtained a total of 344 pictures with 5292 trap-days total sampling effort; in addition, 187 track records in a total of 144 km. With both methods we registered 21 species of mammals, in 13 families and seven orders, and five species resulted in new records to the area. The diversity index of Shannon-Wiener obtained with the method of tracks was H' = 2.41, while the most abundant species were Urocyon cinereoargen- teus (IAR = 0.23) and Pecari tajacu (IAR = 0.20). By the method of trap the most abundant species were P. tajacu (IAR = 2.62) and Nasua narica (IAR = 1.28). In terms of patterns of activity P. tajacu, N. narica and Odocoileus virginianus were primarily diurnal species; Canis latrans and Leopardus pardalis did not show preference for any schedule in particular, and Didelphis virginiana and Dasypus novemcinctus preferred to have nocturnal activity. This information can be of help to the creation of programs of management and conservation of mam- mals of medium and large in the Isthmus of Tehuantepec, Oaxaca, México.

  7. Inconsistency of allometric scaling for dissociative anesthesia of wild felids.

    PubMed

    Carregaro, Adriano B; Freitas, Gabrielle C; Bisetto, Shayne P; Xavier, Nathalia V; Sterzo, Elton V

    2016-05-01

    To evaluate allometric scaling for ketamine-xylazine (KX) anesthesia in wild felids using domestic cats for reference. Prospective single-phase non-blinded study. Six domestic cats and 13 wild felids (five Leopardus pardalis, five Puma concolor, one Panthera onca and two Panthera leo). Six domestic cats (4.1 ± 0.8 kg, REF1) were anesthetized by intramuscular administration of ketamine (15 mg kg(-1) ) and xylazine (1 mg kg(-1) ). Wild cats were divided into three groups based on body weight: 12.9 ± 2.4 kg (G1; n = 7), 43.0 ± 15.7 kg (G2; n = 4) and 126.0 ± 7.8 kg (G3; n = 2). Ketamine and xylazine doses were calculated based on allometric scaling of the basal metabolic rate (BMR = 70 × body mass(0.75) ). Afterwards, the six domestic cats were administered mean KX doses calculated for G1 and G2 (REF2). The heart rate, systolic arterial pressure, respiratory frequency, pH, the venous partial pressure of carbon dioxide, bicarbonate and lactate concentrations were recorded for up to 60 minutes. Additional doses were required in 12 out of the 13 wild cats. Anesthesia was not achieved in G3. Latency periods in wild felids were longer than REF1 and REF2. Anesthesia duration in REF1 was longer than that in the other groups. Recovery from anesthesia in REF1 and REF2 was longer than G1 and G2. Physiological variables remained within the range limits for the species. G1 baseline lactate concentration was higher than in the other groups. KX anesthesia established by allometric scaling of BMR from doses administered to domestic cats did not predict reliable anesthetic doses for wild cats. Dose rates calculated with this method must not be applied to these species. © 2015 Association of Veterinary Anaesthetists and the American College of Veterinary Anesthesia and Analgesia.

  8. Femoral head and neck excision arthroplasty in a leopard tortoise (Stigmochelys pardalis).

    PubMed

    Naylor, Adam D

    2013-12-01

    Cases of femoral head and neck excision arthroplasty are infrequently reported in reptiles, and details of surgical technique and clinical outcome in chelonia are lacking. An adult female leopard tortoise (Stigmochelys pardalis) was presented with chronic non-weight-bearing lameness of the left hind limb. Examination and radiography were consistent with coxofemoral luxation, and as a result of the chronic presentation, surgical intervention was recommended. A cranial approach to the joint via the prefemoral fossa afforded good surgical exposure. A depressed lytic acetabular lesion was noted during the procedure, postulated to be a result of abnormal wear from the luxated femoral head. A fiberglass prop was used during recovery to allow extension of the limb without full weight-bearing. Lameness persisted postoperatively, but limb usage significantly improved.

  9. Insects of bur oak acorns

    Treesearch

    Lester P. Gibson

    1971-01-01

    During 1961-1969, the insects found damaging acorns of bur oak, Quercus macrocarpa Michauxii, in their order of importance were the weevils: Curculio pardalis (Chittenden), C. strictus (Casey), C. sulcatulus (Casey), C. iowensis (Casey), C. proboscideus...

  10. Leopardus braccatus (Carnivora: Felidae)

    USGS Publications Warehouse

    Barstow, Anita L.; Leslie, David M.

    2012-01-01

    Leopardus braccatus (Cope, 1889) is a small felid—not much larger than a domestic house cat—commonly called the Pantanal cat. No comprehensive surveys have been conducted to determine how many L. braccatus exist in the wild. It is found in humid, warm grasslands and wooded areas of extreme northwestern Argentina, southwestern and south- and north-central (newly reported ranges) Brazil, Paraguay, and Uruguay. Habitat loss and degradation are considered major threats throughout most of its range. It is uncommon in captivity and museum collections, listed with all Felidae under Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora, and considered “Near Threatened” by the International Union for Conservation of Nature and Natural Resources in the pampas cat group (= L. colocolo).

  11. CUTANEOUS SQUAMOUS CELL CARCINOMA IN A PANTHER CHAMELEON (FURCIFER PARDALIS) AND TREATMENT WITH CARBOPLATIN IMPLANTABLE BEADS.

    PubMed

    Johnson, James G; Naples, Lisa M; Chu, Caroline; Kinsel, Michael J; Flower, Jennifer E; Van Bonn, William G

    2016-09-01

    A 3-yr-old male panther chameleon (Furcifer pardalis) presented with bilateral raised crusted skin lesions along the lateral body wall that were found to be carcinoma in situ and squamous cell carcinoma. Similar lesions later developed on the caudal body wall and tail. A subcutaneous implantable carboplatin bead was placed in the first squamous cell carcinoma lesion identified. Additional new lesions sampled were also found to be squamous cell carcinomas, and viral polymerase chain reaction was negative for papillomaviruses and herpesviruses. Significant skin loss would have resulted from excision of all the lesions, so treatment with only carboplatin beads was used. No adverse effects were observed. Lesions not excised that were treated with beads decreased in size. This is the first description of cutaneous squamous cell carcinoma and treatment with carboplatin implantable beads in a panther chameleon.

  12. Body size development of captive and free-ranging Leopard tortoises (Geochelone pardalis).

    PubMed

    Ritz, Julia; Hammer, Catrin; Clauss, Marcus

    2010-01-01

    The growth and weight development of Leopard tortoise hatchings (Geochelone pardalis) kept at the Al Wabra Wildlife Preservation (AWWP), Qatar, was observed for more than four years, and compared to data in literature for free-ranging animals on body weight or carapace measurements. The results document a distinctively faster growth in the captive animals. Indications for the same phenomenon in other tortoise species (Galapagos giant tortoises, G. nigra; Spur-thighed tortoises, Testudo graeca; Desert tortoises, Gopherus agassizi) were found in the literature. The cause of the high growth rate most likely is the constant provision with highly digestible food of low fiber content. Increased growth rates are suspected to have negative consequences such as obesity, high mortality, gastrointestinal illnesses, renal diseases, "pyramiding," fibrous osteodystrophy or metabolic bone disease. The apparently widespread occurrence of high growth rates in intensively managed tortoises underlines how easily ectothermic animals can be oversupplemented with nutrients. (c) 2009 Wiley-Liss, Inc.

  13. Elucidating the trophodynamics of four coral reef fishes of the Solomon Islands using δ15N and δ13C

    NASA Astrophysics Data System (ADS)

    Greenwood, N. D. W.; Sweeting, C. J.; Polunin, N. V. C.

    2010-09-01

    Size-related diet shifts are important characteristics of fish trophodynamics. Here, body size-related changes in muscle δ15N and δ13C of four coral reef fishes, Acanthurus nigrofuscus (herbivore), Chaetodon lunulatus (corallivore) , Chromis xanthura (planktivore) and Plectropomus leopardus (piscivore) were investigated at two locations in the Solomon Islands. All four species occupied distinct isotopic niches and the concurrent δ13C' values of C. xanthura and P. leopardus suggested a common planktonic production source. Size-related shifts in δ15N, and thus trophic level, were observed in C. xanthura, C. lunulatus and P. leopardus, and these trends varied between location, indicating spatial differences in trophic ecology. A literature review of tropical fishes revealed that positive δ15N-size trends are common while negative δ15N-size trends are rare. Size-δ15N trends fall into approximately equal groups representing size-based feeding within a food chain, and that associated with a basal resource shift and occurs in conjunction with changes in production source, indicated by δ13C. The review also revealed large scale differences in isotope-size trends and this, combined with small scale location differences noted earlier, highlights a high degree of plasticity in the reef fishes studied. This suggests that trophic size analysis of reef fishes would provide a productive avenue to identify species potentially vulnerable to reef impacts as a result of constrained trophic behaviour.

  14. Recurrent evolution of melanism in South American felids.

    PubMed

    Schneider, Alexsandra; Henegar, Corneliu; Day, Kenneth; Absher, Devin; Napolitano, Constanza; Silveira, Leandro; David, Victor A; O'Brien, Stephen J; Menotti-Raymond, Marilyn; Barsh, Gregory S; Eizirik, Eduardo

    2015-02-01

    Morphological variation in natural populations is a genomic test bed for studying the interface between molecular evolution and population genetics, but some of the most interesting questions involve non-model organisms that lack well annotated reference genomes. Many felid species exhibit polymorphism for melanism but the relative roles played by genetic drift, natural selection, and interspecies hybridization remain uncertain. We identify mutations of Agouti signaling protein (ASIP) or the Melanocortin 1 receptor (MC1R) as independent causes of melanism in three closely related South American species: the pampas cat (Leopardus colocolo), the kodkod (Leopardus guigna), and Geoffroy's cat (Leopardus geoffroyi). To assess population level variation in the regions surrounding the causative mutations we apply genomic resources from the domestic cat to carry out clone-based capture and targeted resequencing of 299 kb and 251 kb segments that contain ASIP and MC1R, respectively, from 54 individuals (13-21 per species), achieving enrichment of ~500-2500-fold and ~150x coverage. Our analysis points to unique evolutionary histories for each of the three species, with a strong selective sweep in the pampas cat, a distinctive but short melanism-specific haplotype in the Geoffroy's cat, and reduced nucleotide diversity for both ancestral and melanism-bearing chromosomes in the kodkod. These results reveal an important role for natural selection in a trait of longstanding interest to ecologists, geneticists, and the lay community, and provide a platform for comparative studies of morphological variation in other natural populations.

  15. Recurrent Evolution of Melanism in South American Felids

    PubMed Central

    Schneider, Alexsandra; Henegar, Corneliu; Day, Kenneth; Absher, Devin; Napolitano, Constanza; Silveira, Leandro; David, Victor A.; O’Brien, Stephen J.; Menotti-Raymond, Marilyn; Barsh, Gregory S.; Eizirik, Eduardo

    2015-01-01

    Morphological variation in natural populations is a genomic test bed for studying the interface between molecular evolution and population genetics, but some of the most interesting questions involve non-model organisms that lack well annotated reference genomes. Many felid species exhibit polymorphism for melanism but the relative roles played by genetic drift, natural selection, and interspecies hybridization remain uncertain. We identify mutations of Agouti signaling protein (ASIP) or the Melanocortin 1 receptor (MC1R) as independent causes of melanism in three closely related South American species: the pampas cat (Leopardus colocolo), the kodkod (Leopardus guigna), and Geoffroy’s cat (Leopardus geoffroyi). To assess population level variation in the regions surrounding the causative mutations we apply genomic resources from the domestic cat to carry out clone-based capture and targeted resequencing of 299 kb and 251 kb segments that contain ASIP and MC1R, respectively, from 54 individuals (13–21 per species), achieving enrichment of ~500–2500-fold and ~150x coverage. Our analysis points to unique evolutionary histories for each of the three species, with a strong selective sweep in the pampas cat, a distinctive but short melanism-specific haplotype in the Geoffroy’s cat, and reduced nucleotide diversity for both ancestral and melanism-bearing chromosomes in the kodkod. These results reveal an important role for natural selection in a trait of longstanding interest to ecologists, geneticists, and the lay community, and provide a platform for comparative studies of morphological variation in other natural populations. PMID:25695801

  16. A novel function of vitellogenin subdomain, vWF type D, as a toxin-binding protein in the pufferfish Takifugu pardalis ovary.

    PubMed

    Yin, Xianzhe; Kiriake, Aya; Ohta, Akira; Kitani, Yoichiro; Ishizaki, Shoichiro; Nagashima, Yuji

    2017-09-15

    Marine pufferfish of the Tetraodontidae family contain high levels of tetrodotoxin (TTX) in the liver and ovary. TTX is suggested to transfer from the liver to the ovary in female pufferfish during maturation. TTX in pufferfish eggs may act as a repellent against predators and as a sexual pheromone to attract male pufferfish. The toxification mechanism of the pufferfish ovary is poorly understood. Here we evaluated the chemical form of TTX and its related substances in the ovary of the panther pufferfish Takifugu pardalis by LC-ESI/MS. TTX and its analogs 4-epi-TTX, 4, 9-anhydroTTX, deoxyTTX, dideoxyTTX, and trideoxyTTX were detected in a low molecular weight fraction by Sephacryl S-400 column chromatography. The finding of an unknown TTX-related substance in a high molecular weight fraction from the Sephacryl S-400 column suggested the occurrence of toxin-binding protein in the ovary. The toxin-binding protein in the ovary was purified by ion-exchange HPLC, gel filtration HPLC, and SDS-PAGE. Amino acid sequencing and cDNA cloning revealed that the toxin-binding protein, TPOBP-10 (Takifugu pardalis ovary toxin-binding protein with a molecular mass of 10 kDa) was homologous with the predicted vitellogenin-1-like protein [Takifugu rubripes] subdomain, a von Willebrand factor type D domain. TPOBP-10 mRNA was highly expressed in the ovary and liver and less in other organs of female individuals based on RT-PCR. These findings reveal a novel function of the vitellogenin subdomain as binding with TTX-related substances, and its involvement in the toxification of the pufferfish ovary. Copyright © 2017 Elsevier Ltd. All rights reserved.

  17. Standardization of some electrocardiographic parameters of captive leopard cats (Leopardus tigrinus).

    PubMed

    Oda, Sam Goldy Shoyama; Yamato, Ronaldo Jun; Fedullo, José Daniel Luzes; Leomil Neto, Moacir; Larsson, Maria Helena Matiko Akao

    2009-09-01

    Thirty-three captive leopard cats, Leopardus tigrinus, were anesthetized with xylazine (1-2 mg/kg) and ketamine (10 mg/kg), and electrocardiograph (ECG) tests were recorded in all leads with 1 cm = 1 mV sensibility and 25 mm/sec speed repeating DII lead at 50 mm/sec speed with the same sensibility. Results expressed by mean and standard deviation were: heart rate (HR) = 107 +/- 17 (bpm); P-wave = 0.048 +/- 0.072 (s) x 0.128 +/- 0.048 (mV); PR interval = 0.101 +/- 0.081 (s); QRS compound = 0.053 +/- 0.012 (s) x 1.446 +/- 0.602 (mV); QT interval = 0.231 +/- 0.028 (s); R-wave (CV6LL) = 1.574 +/- 0.527 (mV); R-wave (CV6LU) = 1.583 +/- 0.818 (mV); heart rhythm: normal sinus rhythm (15.2%), sinus rhythm with wandering pacemaker (WPM) (60.6%), sinus arrhythmia with WPM (24.2%); electric axis: between +30 degrees and +60 degrees (6.1%), +60 (6.1%), between +60 degrees and +90 degrees (57.6%), +90 degrees (9%), between +90 degrees and +120 degrees (21.2%); ST segment: normal (75.7%), elevation (18.2%), depression (6.1%); T-wave polarity (DII): positive (100%); T-wave (V10): absent (6.1%), negative (63.6%), positive (18.2%), and with interference (12.1%). Through ECG data comparison with other species, unique features of Leopardus tigrinus' (leopard cat) ECG parameters were detected. Some of the study animals presented with an R-wave amplitude that was indicative of left ventricle overload according to patterns for normal domestic cats (Felis cati). Echocardiographic exams revealed normal heart cavities' function and morphology. The aim of this study was to establish some electrocardiographic parameters of captive L. tigrinus.

  18. Capillaria plectropomi n. sp. (Nematoda: Capillariidae), a new intestinal parasite of the leopard coral grouper Plectropomus leopardus (Serranidae) off New Caledonia

    PubMed Central

    Moravec, František; Justine, Jean-Lou

    2014-01-01

    A new nematode species, Capillaria plectropomi n. sp. (Capillariidae), is described from the intestine of the leopard coral grouper Plectropomus leopardus (Lacepède) from coral reefs off New Caledonia. The new species, belonging to the subgenus Neocapillaria Moravec, 1987, differs from other congeneric species of this subgenus from marine fishes mainly in the length (168–186 μm), shape and structure of the spicule. It is characterized, in the male, by the presence of two well-developed dorsolateral caudal lobes, a pair of lateral papillae, a heavily sclerotized spicule with many rough transverse grooves in the middle part, a spinose spicular sheath, and in the female, by eggs measuring 60–66 × 27 μm without protruding polar plugs. The buccal cavity contains a small finger-shaped stylet. Capillaria plectropomi n. sp. is the first known species of this genus parasitizing fishes of the perciform family Serranidae. PMID:25531932

  19. Taxonomic revision of the species of Parvanachis Radwin, 1968 (Gastropoda: Columbellidae) from the Gulf of Panama.

    PubMed

    de Maintenon, Marta J

    2014-01-07

    Species of Parvanachis Radwin, 1968 collected from the Gulf of Panama are anatomically characterized and taxonomically revised. Six species are reported to occur in the region: P. pygmaea (Sowerby, 1832), P. pardalis (Hinds, 1843), P. diminuta (C.B. Adams, 1852), P. albonodosa (Carpenter, 1857), P. milium (Dall, 1916), and P. dalli Bartsch, 1931. Of these six, three are confirmed as described. P. milium was not found in new collections or the previous survey collection and may be more common further south. P. dalli is a synonym of P. pygmaea; and P. albonodosa (auct.) is renamed, because the type material represents a different species. Two new species are described, P. dichroma and P. adamsi. P. pardalis, based on anatomy and shell and radular morphology, is referred to the genus Anachis, and Costoanachis nigricans (Sowerby, 1844), based on anatomy and shell morphology, is transferred to Parvanachis. The resulting seven species of Parvanachis constitute two groups; one (including the type species) with smaller shells and a simple penis morphology, and a group of four larger species with more complex penis morphology.

  20. Aquatic Resources of Rocky Mountain Arsenal Adams County, Colorado

    DTIC Science & Technology

    1989-09-01

    D Nais pardalis P Nais simplex D,P D,P DUNais variabilis D,P D,P P Ophidonais serpentina D D,P Pristina leidyi D D Stylaria lacustris D D D... serpentina were the dominant naidids in Lake Ladora. Nais variabilis was the only naidid identified in Lake Mary. Most of the tubificid worms collected were

  1. Effects of dietary Spirulina platensis on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and disease resistance of Coral trout Plectropomus leopardus (Lacepede, 1802).

    PubMed

    Yu, Wei; Wen, Guoliang; Lin, Heizhao; Yang, Yukai; Huang, Xiaolin; Zhou, Chuanpeng; Zhang, Zaiwang; Duan, Yafei; Huang, Zhong; Li, Tao

    2018-03-01

    The present study investigated the effects of dietary Spirulina platensis supplementation on growth performance, hematological and serum biochemical parameters, hepatic antioxidant status, immune responses and resistance to the pathogen infection in Coral trout Plectropomus leopardus. The fish were fed for 8-week with diets containing different levels of S. platensis: 0% (C), 2% (SP2), 4% (SP4), 6% (SP6), 8% (SP8) and 10% (SP10) as treatment groups, followed by a Vibrio harveyi infection test for 14 d. The study indicated that dietary supplementation with Spirulina platensis could significantly improve growth performance, and the highest weight gain rate (WGR) and specific growth rate (SGR) were observed in group SP10 (P < .05). Red cell count (RBC), white cell count (WBC), hemoglobin (Hb) and total antioxidant capacity (T-AOC) in the S. platensis supplemented groups were significantly higher than those of group C (P < .05). However, the levels of cholesterol, triglyceride and malondialdehyde (MDA) contents, and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activities decreased with the increasing of dietary S. platensis levels. Compared with group C, the lysozyme (LYZ) and respiratory burst activities (RBA), and immunoglobulin (Ig) and complement contents in group SP4, SP6, SP8 and SP10 increased significantly than those of group C respectively (P < .05). After challenge with V. harveyi, the survival rate in group SP4, SP6, SP8 and SP10 was significantly higher than that of group C, and the highest survival rate was in group SP10 (P < .05). These results indicated that P. leopardus fed a diet supplemented with S. platensis (especially at 10%) could significantly promote its growth performance, improve its hepatic antioxidant status, and enhance its immune ability and resistance to V. harveyi infection. Copyright © 2018 Elsevier Ltd. All rights reserved.

  2. LC/MS Analysis of Tetrodotoxin and Its Deoxy Analogs in the Marine Puffer Fish Fugu niphobles from the Southern Coast of Korea, and in the Brackishwater Puffer Fishes Tetraodon nigroviridis and Tetraodon biocellatus from Southeast Asia

    PubMed Central

    Jang, Jun-Ho; Lee, Jong-Soo; Yotsu-Yamashita, Mari

    2010-01-01

    Tetrodotoxin (TTX) and its deoxy analogs, 5-deoxyTTX, 11-deoxyTTX, 6,11-dideoxyTTX, and 5,6,11-trideoxyTTX, were quantified in the tissues of three female and three male specimens of the marine puffer fish, Fugu niphobles, from the southern coast of Korea, and in the whole body of the brackishwater puffer fishes, Tetraodon nigroviridis (12 specimens) and Tetrodon biocellatus (three specimens) from Southeast Asia using LC/MS in single ion mode (SIM). Identification of these four deoxy analogs in the ovarian tissue of F. niphobles were further confirmed by LC/MS/MS. TTX and 5,6,11-trideoxyTTX were detected in all three puffer fish species as the major TTX analogs, similar to Japanese Fugu pardalis. While 6,11-dideoxyTTX was also found to be a major analog in almost all tissues of Korean F. niphobles, this analog was minor in the two Tetraodon species and Japanese F. pardalis. Among the tissues of F. niphobles, the concentrations of TTXs were highest in the ovaries (female) and skin (female and male). PMID:20479966

  3. Maternal-embryonic metabolic and antioxidant response of Chapalichthys pardalis (Teleostei: Goodeidae) induced by exposure to 3,4-dichloroaniline.

    PubMed

    Carbajal-Hernández, Ana Laura; Valerio-García, Roberto Carlos; Martínez-Ruíz, Erika Berenice; Jarquín-Díaz, Víctor Hugo; Martínez-Jerónimo, Fernando

    2017-07-01

    Chapalichthys pardalis is a viviparous fish, microendemic to the Tocumbo Region in the state of Michoacán, Mexico. Despite the peculiar type of reproduction of goodeid fish and their mother-embryo interaction, the effects on embryos induced by maternal exposure to aquatic xenobiotics are still unknown. The objective of the present work was to determine the maternal-embryonic metabolic and antioxidant response of C. pardalis exposed to 3,4-dichloroaniline (3,4-DCA), a compound considered highly noxious to the environment because of its high toxicity and persistence, which has been used as reference toxicant in toxicological bioassays. We determined the median lethal concentration (LC 50 , 96 h) and then exposed pregnant females to 3.3, 2.5, and 0.5 mg L -1 of 3,4-DCA (equivalent to LC 1 , LC 0.01 , and LC 50/10 , respectively) during 21 days. We assessed the activity of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), macromolecules content (proteins, lipids, carbohydrates), glucose, and lactate concentration, as well as the oxidative damage, by measuring thiobarbituric acid reactive substances, and protein oxidation. To interpret results, we used the integrated biomarker response (IBRv2). The average LC 50 was of 5.18 mg L -1 (4.8-5.5 mg L -1 ; p = 0.05). All females exposed to concentrations of 3.3 and 2.5 mg L -1 lost 100% of the embryos during the bioassay, whereas those exposed to 0.5 mg L -1 showed alterations in the antioxidant activity and oxidative damage, being the embryos and the maternal liver the most affected, with IBRv2 values of 10.09 and 9.21, respectively. Damage to macromolecules was greater in embryos and the maternal liver, with IBRv2 of 16.14 and 8.40, respectively. We conclude that exposure to xenobiotics, like 3,4-DCA, in species with a marked maternal-embryonic interaction represents a potential risk for the development and survival of the descendants, thereby, potentially affecting the future of the population.

  4. Effects of region, demography, and protection from fishing on batch fecundity of common coral trout ( Plectropomus leopardus)

    NASA Astrophysics Data System (ADS)

    Carter, Alex B.; Davies, Campbell R.; Mapstone, Bruce D.; Russ, Garry R.; Tobin, Andrew J.; Williams, Ashley J.

    2014-09-01

    Batch fecundity of female Plectropomus leopardus, a coral reef fish targeted by commercial and recreational fishing, was compared between reefs open to fishing and reefs within no-take marine reserves within three regions of the Great Barrier Reef (GBR), Australia. Length, weight, and age had positive effects on batch fecundity of spawners from northern and central reefs but negligible effects on spawners from southern reefs. Females were least fecund for a given length, weight, and age in the southern GBR. Batch fecundity of a 500-mm fork length female was 430 % greater on central reefs and 207 % greater on northern reefs than on southern reefs. The effects of length and age on batch fecundity did not differ significantly between reserve and fished reefs in any region, but weight-specific fecundity was 100 % greater for large 2.0 kg females on reserve reefs compared with fished reefs in the central GBR. We hypothesize that regional variation in batch fecundity is likely driven by water temperature and prey availability. Significant regional variation in batch fecundity highlights the need for understanding spatial variation in reproductive output where single conservation or fishery management strategies cover large, potentially diverse, spatial scales.

  5. Carbohydrate management, anaerobic metabolism, and adenosine levels in the armoured catfish, Liposarcus pardalis (castelnau), during hypoxia.

    PubMed

    Maccormack, Tyson James; Lewis, Johanne Mari; Almeida-Val, Vera Maria Fonseca; Val, Adalberto Luis; Driedzic, William Robert

    2006-04-01

    The armoured catfish, Liposarcus pardalis, tolerates severe hypoxia at high temperatures. Although this species can breathe air, it also has a strong anaerobic metabolism. We assessed tissue to plasma glucose ratios and glycogen and lactate in a number of tissues under "natural" pond hypoxia, and severe aquarium hypoxia without aerial respiration. Armour lactate content and adenosine in brain and heart were also investigated. During normoxia, tissue to plasma glucose ratios in gill, brain, and heart were close to one. Hypoxia increased plasma glucose and decreased tissue to plasma ratios to less than one, suggesting glucose phosphorylation is activated more than uptake. High normoxic white muscle glucose relative to plasma suggests gluconeogenesis or active glucose uptake. Excess muscle glucose may serve as a metabolic reserve since hypoxia decreased muscle to plasma glucose ratios. Mild pond hypoxia changed glucose management in the absence of lactate accumulation. Lactate was elevated in all tissues except armour following aquarium hypoxia; however, confinement in aquaria increased armour lactate, even under normoxia. A stress-associated acidosis may contribute to armour lactate sequestration. High plasma lactate levels were associated with brain adenosine accumulation. An increase in heart adenosine was triggered by confinement in aquaria, although not by hypoxia alone.

  6. Semen collection and ejaculate characteristics of the Leopard Tortoise (Stigmochelys pardalis)

    PubMed Central

    Mitchell, Mark A

    2017-01-01

    Abstract The preservation of spermatozoa is an important tool used in conservation programs to increase the genetic diversity of threatened and endangered species. Although routinely used to manage conservation programs for higher vertebrates, there have been limited attempts to establish reproductive assistance programs for tortoises. The purpose of this study was to develop a model for collecting and characterizing semen in Testudinidae. Semen was collected from 13/16 (81.2%, 95% CI: 62–100) adult male leopard tortoises (Stigmochelys pardalis) via electroejaculation under propofol anesthesia. Semen samples were collected most frequently after the second series of electrostimulations (6/13, 46.1%), with fewer animals producing semen after the first (5/13, 38.5%) or third (2/13, 15.4%) electrostimulations. The average volume of a semen sample in the tortoises was 0.26 ml (standard deviation: 0.16, minimum–maximum: 0.1–0.6), the average spermatozoal concentration was 101.62 × 106/ml, and the average motility at time of collection was 57.3%. A rapid decrease in motility was observed in refrigerated samples over 24 h resulting in a median motility of 0% at 24 h post-collection. The results of this study suggest that electroejaculation is a safe and efficient method for collecting semen from leopard tortoises. PMID:29230293

  7. Genetic characterization of Toxoplasma gondii from Brazilian wildlife revealed abundant new genotypes.

    PubMed

    Vitaliano, S N; Soares, H S; Minervino, A H H; Santos, A L Q; Werther, K; Marvulo, M F V; Siqueira, D B; Pena, H F J; Soares, R M; Su, C; Gennari, S M

    2014-12-01

    This study aimed to isolate and genotype T. gondii from Brazilian wildlife. For this purpose, 226 samples were submitted to mice bioassay and screened by PCR based on 18S rRNA sequences. A total of 15 T. gondii isolates were obtained, including samples from four armadillos (three Dasypus novemcinctus, one Euphractus sexcinctus), three collared anteaters (Tamandua tetradactyla), three whited-lipped peccaries (Tayassu pecari), one spotted paca (Cuniculus paca), one oncilla (Leopardus tigrinus), one hoary fox (Pseudalopex vetulus), one lineated woodpecker (Dryocopus lineatus) and one maned wolf (Chrysocyon brachyurus). DNA from the isolates, originated from mice bioassay, and from the tissues of the wild animal, designated as "primary samples", were genotyped by PCR-restriction fragment length polymorphism (PCR/RFLP), using 12 genetic markers (SAG1, SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L258, PK1, CS3 and Apico). A total of 17 genotypes were identified, with 13 identified for the first time and four already reported in published literature. Results herein obtained corroborate previous studies in Brazil, confirming high diversity and revealing unique genotypes in this region. Given most of genotypes here identified are different from previous studies in domestic animals, future studies on T. gondii from wildlife is of interest to understand population genetics and structure of this parasite.

  8. Genetic characterization of Toxoplasma gondii from Brazilian wildlife revealed abundant new genotypes

    PubMed Central

    Vitaliano, S.N.; Soares, H.S.; Minervino, A.H.H.; Santos, A.L.Q.; Werther, K.; Marvulo, M.F.V.; Siqueira, D.B.; Pena, H.F.J.; Soares, R.M.; Su, C.; Gennari, S.M.

    2014-01-01

    This study aimed to isolate and genotype T. gondii from Brazilian wildlife. For this purpose, 226 samples were submitted to mice bioassay and screened by PCR based on 18S rRNA sequences. A total of 15 T. gondii isolates were obtained, including samples from four armadillos (three Dasypus novemcinctus, one Euphractus sexcinctus), three collared anteaters (Tamandua tetradactyla), three whited-lipped peccaries (Tayassu pecari), one spotted paca (Cuniculus paca), one oncilla (Leopardus tigrinus), one hoary fox (Pseudalopex vetulus), one lineated woodpecker (Dryocopus lineatus) and one maned wolf (Chrysocyon brachyurus). DNA from the isolates, originated from mice bioassay, and from the tissues of the wild animal, designated as “primary samples”, were genotyped by PCR–restriction fragment length polymorphism (PCR/RFLP), using 12 genetic markers (SAG1, SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L258, PK1, CS3 and Apico). A total of 17 genotypes were identified, with 13 identified for the first time and four already reported in published literature. Results herein obtained corroborate previous studies in Brazil, confirming high diversity and revealing unique genotypes in this region. Given most of genotypes here identified are different from previous studies in domestic animals, future studies on T. gondii from wildlife is of interest to understand population genetics and structure of this parasite. PMID:25426424

  9. Feline immunodeficiency virus and feline leukemia virus infection in free-ranging guignas (Leopardus guigna) and sympatric domestic cats in human perturbed landscapes on Chiloé Island, Chile.

    PubMed

    Mora, Mónica; Napolitano, Constanza; Ortega, René; Poulin, Elie; Pizarro-Lucero, José

    2015-01-01

    Feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) are two of the most common viruses affecting domestic cats (Felis catus). During the last two decades, reports show that both viruses also infect or affect other species of the family Felidae. Human landscape perturbation is one of the main causes of emerging diseases in wild animals, facilitating contact and transmission of pathogens between domestic and wild animals. We investigated FIV and FeLV infection in free-ranging guignas (Leopardus guigna) and sympatric domestic cats in human perturbed landscapes on Chiloé Island, Chile. Samples from 78 domestic cats and 15 guignas were collected from 2008 to 2010 and analyzed by PCR amplification and sequencing. Two guignas and two domestic cats were positive for FIV; three guignas and 26 domestic cats were positive for FeLV. The high percentage of nucleotide identity of FIV and FeLV sequences from both species suggests possible interspecies transmission of viruses, facilitated by increased contact probability through human invasion into natural habitats, fragmentation of guigna habitat, and poultry attacks by guignas. This study enhances our knowledge on the transmission of pathogens from domestic to wild animals in the global scenario of human landscape perturbation and emerging diseases.

  10. The multiple and complex and changeable scenarios of the Trypanosoma cruzi transmission cycle in the sylvatic environment.

    PubMed

    Jansen, Ana Maria; Xavier, Samanta C C; Roque, André Luiz R

    2015-11-01

    In this study, we report and discuss the results generated from over 20 years of studies of the Trypanosoma cruzi sylvatic transmission cycle. Our results have uncovered new aspects and reviewed old concepts on issues including reservoirs, true generalist species, association of mammalian species with distinct discrete typing units - DTUs, distribution of T. cruzi genotypes in the wild, mixed infections, and T. cruzi transmission ecology. Using parasitological and serological tests, we examined T. cruzi infection in 7,285 mammalian specimens from nine mammalian orders dispersed all over the Brazilian biomes. The obtained T. cruzi isolates were characterized by mini-exon gene sequence polymorphism and PCR RFLP to identify DTUs. Infection by T. cruzi was detected by serological methods in 20% of the examined animals and isolated from 41% of those infected, corresponding to 8% of all the examined mammals. Each mammal taxon responded uniquely to T. cruzi infection. Didelphis spp. are able to maintain high and long-lasting parasitemias (positive hemocultures) caused by TcI but maintain and rapidly control parasitemias caused by TcII to almost undetectable levels. In contrast, the tamarin species Leontopithecus rosalia and L. chrysomelas maintain long-lasting and high parasitemias caused by TcII similarly to Philander sp. The coati Nasua nasua maintains high parasitemias by both parental T. cruzi DTUs TcI or TcII and by TcII/TcIV (formerly Z3) at detectable levels. Wild and domestic canidae seem to display only a short period of reservoir competence. T. cruzi infection was demonstrated in the wild canid species Cerdocyon thous and Chrysocyon brachyurus, and positive hemoculture was obtained in one hyper carnivore species (Leopardus pardalis), demonstrating that T. cruzi transmission is deeply immersed in the trophic net. T. cruzi DTU distribution in nature did not exhibit any association with a particular biome or habitat. TcI predominates throughout (58% of the T. cruzi isolates); however, in spite of being significantly less frequent (17%), TcII is also widely distributed. Concomitant DTU infection occurred in 16% of infected mammals of all biomes and included arboreal and terrestrial species, as well as bats. TcI/TcII concomitant infection was the most common and widely dispersed, with mixed TcI/TcII infections especially common in coatis and in Didelphimorphia. The second most common pattern of concomitant infection was TcI/TcIV, observed in Chiroptera, Didelphimorphia and Primates. Taken together, our results demonstrate the complexity of T. cruzi reservoir system and its transmission strategies, indicating that there is considerably more to be learned regarding ecology of T. cruzi. Copyright © 2015 Elsevier B.V. All rights reserved.

  11. Macroinvertebrates of Luxapalila Creek, Mississippi and Alabama, 1987-89

    DTIC Science & Technology

    1991-02-01

    invertebrates were identified to the lowest possible taxon with appropriate keys. Voucher speci- mens have been retained at the US Army Engineer Waterways...unidenta 0 0.0000 4 0.0065 Chaetogaster diaphanus 3 0.0080 51 0.0831 Dero digitata 3 0.0080 10 0.0163 Dero furcata 13 0.0347 0 0.0000 Dero nivea 8...and included Nais pardalis (14.7 percent), Dero nivea (12.7 percent), D. obtusa (12.4 percent), N. variables (8.6 percent), Pristinella osborni (8.5

  12. Reduced Genetic Diversity and Increased Dispersal in Guigna (Leopardus guigna) in Chilean Fragmented Landscapes.

    PubMed

    Napolitano, Constanza; Díaz, Diego; Sanderson, Jim; Johnson, Warren E; Ritland, Kermit; Ritland, Carol E; Poulin, Elie

    2015-01-01

    Landscape fragmentation is often a major cause of species extinction as it can affect a wide variety of ecological processes. The impact of fragmentation varies among species depending on many factors, including their life-history traits and dispersal abilities. Felids are one of the groups most threatened by fragmented landscapes because of their large home ranges, territorial behavior, and low population densities. Here, we model the impacts of habitat fragmentation on patterns of genetic diversity in the guigna (Leopardus guigna), a small felid that is closely associated with the heavily human-impacted temperate rainforests of southern South America. We assessed genetic variation in 1798 base pairs of mitochondrial DNA sequences, 15 microsatellite loci, and 2 sex chromosome genes and estimated genetic diversity, kinship, inbreeding, and dispersal in 38 individuals from landscapes with differing degrees of fragmentation on Chiloé Island in southern Chile. Increased fragmentation was associated with reduced genetic diversity, but not with increased kinship or inbreeding. However, in fragmented landscapes, there was a weaker negative correlation between pairwise kinship and geographic distance, suggesting increased dispersal distances. These results highlight the importance of biological corridors to maximize connectivity in fragmented landscapes and contribute to our understanding of the broader genetic consequences of habitat fragmentation, especially for forest-specialist carnivores. © The American Genetic Association 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  13. Estimating detection and density of the Andean cat in the high Andes

    USGS Publications Warehouse

    Reppucci, J.; Gardner, B.; Lucherini, M.

    2011-01-01

    The Andean cat (Leopardus jacobita) is one of the most endangered, yet least known, felids. Although the Andean cat is considered at risk of extinction, rigorous quantitative population studies are lacking. Because physical observations of the Andean cat are difficult to make in the wild, we used a camera-trapping array to photo-capture individuals. The survey was conducted in northwestern Argentina at an elevation of approximately 4,200 m during October-December 2006 and April-June 2007. In each year we deployed 22 pairs of camera traps, which were strategically placed. To estimate detection probability and density we applied models for spatial capture-recapture using a Bayesian framework. Estimated densities were 0.07 and 0.12 individual/km 2 for 2006 and 2007, respectively. Mean baseline detection probability was estimated at 0.07. By comparison, densities of the Pampas cat (Leopardus colocolo), another poorly known felid that shares its habitat with the Andean cat, were estimated at 0.74-0.79 individual/km2 in the same study area for 2006 and 2007, and its detection probability was estimated at 0.02. Despite having greater detectability, the Andean cat is rarer in the study region than the Pampas cat. Properly accounting for the detection probability is important in making reliable estimates of density, a key parameter in conservation and management decisions for any species. ?? 2011 American Society of Mammalogists.

  14. Estimating detection and density of the Andean cat in the high Andes

    USGS Publications Warehouse

    Reppucci, Juan; Gardner, Beth; Lucherini, Mauro

    2011-01-01

    The Andean cat (Leopardus jacobita) is one of the most endangered, yet least known, felids. Although the Andean cat is considered at risk of extinction, rigorous quantitative population studies are lacking. Because physical observations of the Andean cat are difficult to make in the wild, we used a camera-trapping array to photo-capture individuals. The survey was conducted in northwestern Argentina at an elevation of approximately 4,200 m during October–December 2006 and April–June 2007. In each year we deployed 22 pairs of camera traps, which were strategically placed. To estimate detection probability and density we applied models for spatial capture–recapture using a Bayesian framework. Estimated densities were 0.07 and 0.12 individual/km2 for 2006 and 2007, respectively. Mean baseline detection probability was estimated at 0.07. By comparison, densities of the Pampas cat (Leopardus colocolo), another poorly known felid that shares its habitat with the Andean cat, were estimated at 0.74–0.79 individual/km2 in the same study area for 2006 and 2007, and its detection probability was estimated at 0.02. Despite having greater detectability, the Andean cat is rarer in the study region than the Pampas cat. Properly accounting for the detection probability is important in making reliable estimates of density, a key parameter in conservation and management decisions for any species.

  15. Blood parasites in reptiles imported to Germany.

    PubMed

    Halla, Ursula; Ursula, Halla; Korbel, Rüdiger; Rüdiger, Korbel; Mutschmann, Frank; Frank, Mutschmann; Rinder, Monika; Monika, Rinder

    2014-12-01

    Though international trade is increasing, the significance of imported reptiles as carriers of pathogens with relevance to animal and human health is largely unknown. Reptiles imported to Germany were therefore investigated for blood parasites using light microscopy, and the detected parasites were morphologically characterized. Four hundred ten reptiles belonging to 17 species originating from 11 Asian, South American and African countries were included. Parasites were detected in 117 (29%) of individual reptiles and in 12 species. Haemococcidea (Haemogregarina, Hepatozoon, Schellackia) were found in 84% of snakes (Python regius, Corallus caninus), 20% of lizards (Acanthocercus atricollis, Agama agama, Kinyongia fischeri, Gekko gecko) and 50% of turtles (Pelusios castaneus). Infections with Hematozoea (Plasmodium, Sauroplasma) were detected in 14% of lizards (Acanthocercus atricollis, Agama agama, Agama mwanzae, K. fischeri, Furcifer pardalis, Xenagama batillifera, Acanthosaura capra, Physignathus cocincinus), while those with Kinetoplastea (Trypanosoma) were found in 9% of snakes (Python regius, Corallus caninus) and 25 % of lizards (K. fischeri, Acanthosaura capra, G. gecko). Nematoda including filarial larvae parasitized in 10% of lizards (Agama agama, Agama mwanzae, K. fischeri, Fu. pardalis, Physignathus cocincinus). Light microscopy mostly allowed diagnosis of the parasites' genus, while species identification was not possible because of limited morphological characteristics available for parasitic developmental stages. The investigation revealed a high percentage of imported reptiles being carriers of parasites while possible vectors and pathogenicity are largely unknown so far. The spreading of haemoparasites thus represents an incalculable risk for pet reptiles, native herpetofauna and even human beings.

  16. Comparative myology of the ankle of Leopardus wiedii and L. geoffroyi (Carnivora: Felidae): functional consistency with osteology, locomotor habits and hunting in captivity.

    PubMed

    Morales, Miriam M; Moyano, S Rocío; Ortiz, Agustina M; Ercoli, Marcos D; Aguado, Luis I; Cardozo, Sergio A; Giannini, Norberto P

    2018-02-01

    Leopardus wiedii (margay) is the only arboreal Neotropical felid able to climb head-first down trees, due to its ability to rotate its tarsal joint 180°. A closely related, similar-sized species, L. geoffroyi (Geoffroy's cat) exhibits more typical terrestrial habits and lacks the arboreal capabilities of L. wiedii. There is osteological evidence that supports a mechanical specialization of L. wiedii's tarsal joint for inversion, but there have been no studies on the myology of this specialization. Based on comparative gross-anatomy dissections of zeugo- and autopodial muscles related to the ankle joint of one margay specimen and two Geoffroýs cats, we identified myological specializations of L. wiedii that support its arboreal abilities. In addition, we documented both species hunting the same prey (domestic pigeon Columba livia, Aves: Columbidae) in captivity, to complement. We report differences in the origin, insertion and belly in 8 of the 10 dissected muscles. At least 3 of these interspecific variations can be associated with strengthening of the main muscles that command inversion/eversion movements of the tarsal joint and support the body weight in the head-down climbing position typical of L. wiedii. Frame-by-frame video reconstructions depict the sequence of movements in these species while hunting and highlight the advantages of the arboreal abilities of L. wiedii. Copyright © 2017 Elsevier GmbH. All rights reserved.

  17. An international borderland of concern: Conservation of biodiversity in the Lower Rio Grande Valley

    USGS Publications Warehouse

    Leslie,, David M.

    2016-07-20

    The Lower Rio Grande Valley (LRGV) of southern Texas is located on the United States-Mexico borderland and represents a 240-kilometer (150-mile) linear stretch that ends at the Gulf of Mexico. The LRGV represents a unique transition between temperate and tropical conditions and, as such, sustains an exceptionally high diversity of plants and animals—some of them found in few, or no other, places in the United States. Examples include Leopardus pardalis albescens (northern ocelot) and Falco femoralis septentrionalis (northern aplomado falcon)—both endangered in the United States and emblematic of the LRGV. The U.S. Fish and Wildlife Service (USFWS) manages three national wildlife refuges (Santa Ana, Lower Rio Grande Valley, and Laguna Atascosa) that together make up the South Texas Refuge Complex, which actively conserves biodiversity in about 76,006 hectares (187,815.5 acres) of native riparian and upland habitats in the LRGV. These diminished habitats harbor many rare, threatened, and endangered species. This report updates the widely used 1988 USFWS biological report titled “Tamaulipan Brushland of the Lower Rio Grande Valley of South Texas: Description, Human Impacts, and Management Options” by synthesizing nearly 400 peer-reviewed scientific publications that have resulted from biological and sociological research conducted specifically in the four Texas counties of the LRGV in the past nearly 30 years. This report has three goals: (1) synthesize scientific insights gained since 1988 related to the biology and management of the LRGV and its unique biota, focusing on flora and fauna of greatest conservation concern; (2) update ongoing challenges facing Federal and State agencies and organizations that focus on conservation or key natural resources in the LRGV; and (3) redefine conservation opportunities and land-acquisition strategies that are feasible and appropriate today, given the many new and expanding constraints that challenge conservation activities in the LRGV. The LRGV faces every contemporary conservation challenge of the 21st century, but ongoing human population growth and its associated demands, international border issues, and oil, gas, and alternative energy development dominate impacts that affect conservation in the LRGV. Continued careful syntheses of existing and future information collected in the LRGV are needed on many biological and sociological topics to guide conservation activities. Quick response will no doubt be necessary to face contemporary and difficult-to-predict challenges such as climate change, diminished water availability and quality, spread of invasive species, and habitat loss and fragmentation. Complexities of a guarded international borderland add pressure to small patches of native habitat that remain in many places of the LRGV, particularly along the Rio Grande. Large connected corridors of restored native habitat could be the best option to maintain, and even enhance, the exceptional biodiversity of the LRGV in the face of exceptional human demand.

  18. The behavioural response of adult Petromyzon marinus to damage-released alarm and predator cues

    USGS Publications Warehouse

    Imre, István; Di Rocco, Richard; Belanger, Cowan; Brown, Grant; Johnson, Nicholas S.

    2014-01-01

    Using semi-natural enclosures, this study investigated (1) whether adult sea lamprey Petromyzon marinus show avoidance of damage-released conspecific cues, damage-released heterospecific cues and predator cues and (2) whether this is a general response to injured heterospecific fishes or a specific response to injured P. marinus. Ten replicate groups of 10 adult P. marinus, separated by sex, were exposed to one of the following nine stimuli: deionized water (control), extracts prepared from adult P. marinus, decayed adult P. marinus (conspecific stimuli), sympatric white sucker Catostomus commersonii, Amazon sailfin catfish Pterygoplichthys pardalis (heterospecific stimuli), 2-phenylethylamine (PEA HCl) solution, northern water snake Nerodia sipedon washing, human saliva (predator cues) and an adult P. marinus extract and human saliva combination (a damage-released conspecific cue and a predator cue). Adult P. marinus showed a significant avoidance response to the adult P. marinus extract as well as to C. commersonii, human saliva, PEA and the adult P. marinus extract and human saliva combination. For mobile P. marinus, the N. sipedon washing induced behaviour consistent with predator inspection. Exposure to the P. pardalis extract did not induce a significant avoidance response during the stimulus release period. Mobile adult female P. marinus showed a stronger avoidance behaviour than mobile adult male P. marinus in response to the adult P. marinus extract and the adult P. marinus extract and human saliva combination. The findings support the continued investigation of natural damage-released alarm cue and predator-based repellents for the behavioural manipulation of P. marinus populations in the Laurentian Great Lakes.

  19. In Silico Comparative Transcriptome Analysis of Two Color Morphs of the Common Coral Trout (Plectropomus Leopardus)

    PubMed Central

    Wang, Le; Yu, Cuiping; Guo, Liang; Lin, Haoran; Meng, Zining

    2015-01-01

    The common coral trout is one species of major importance in commercial fisheries and aquaculture. Recently, two different color morphs of Plectropomus leopardus were discovered and the biological importance of the color difference is unknown. Since coral trout species are poorly characterized at the molecular level, we undertook the transcriptomic characterization of the two color morphs, one black and one red coral trout, using Illumina next generation sequencing technologies. The study produced 55162966 and 54588952 paired-end reads, for black and red trout, respectively. De novo transcriptome assembly generated 95367 and 99424 unique sequences in black and red trout, respectively, with 88813 sequences shared between them. Approximately 50% of both trancriptomes were functionally annotated by BLAST searches against protein databases. The two trancriptomes were enriched into 25 functional categories and showed similar profiles of Gene Ontology category compositions. 34110 unigenes were grouped into 259 KEGG pathways. Moreover, we identified 14649 simple sequence repeats (SSRs) and designed primers for potential application. We also discovered 130524 putative single nucleotide polymorphisms (SNPs) in the two transcriptomes, supplying potential genomic resources for the coral trout species. In addition, we identified 936 fast-evolving genes and 165 candidate genes under positive selection between the two color morphs. Finally, 38 candidate genes underlying the mechanism of color and pigmentation were also isolated. This study presents the first transcriptome resources for the common coral trout and provides basic information for the development of genomic tools for the identification, conservation, and understanding of the speciation and local adaptation of coral reef fish species. PMID:26713756

  20. The behavioural response of adult Petromyzon marinus to damage-released alarm and predator cues.

    PubMed

    Imre, I; Di Rocco, R T; Belanger, C F; Brown, G E; Johnson, N S

    2014-05-01

    Using semi-natural enclosures, this study investigated (1) whether adult sea lamprey Petromyzon marinus show avoidance of damage-released conspecific cues, damage-released heterospecific cues and predator cues and (2) whether this is a general response to injured heterospecific fishes or a specific response to injured P. marinus. Ten replicate groups of 10 adult P. marinus, separated by sex, were exposed to one of the following nine stimuli: deionized water (control), extracts prepared from adult P. marinus, decayed adult P. marinus (conspecific stimuli), sympatric white sucker Catostomus commersonii, Amazon sailfin catfish Pterygoplichthys pardalis (heterospecific stimuli), 2-phenylethylamine (PEA HCl) solution, northern water snake Nerodia sipedon washing, human saliva (predator cues) and an adult P. marinus extract and human saliva combination (a damage-released conspecific cue and a predator cue). Adult P. marinus showed a significant avoidance response to the adult P. marinus extract as well as to C. commersonii, human saliva, PEA and the adult P. marinus extract and human saliva combination. For mobile P. marinus, the N. sipedon washing induced behaviour consistent with predator inspection. Exposure to the P. pardalis extract did not induce a significant avoidance response during the stimulus release period. Mobile adult female P. marinus showed a stronger avoidance behaviour than mobile adult male P. marinus in response to the adult P. marinus extract and the adult P. marinus extract and human saliva combination. The findings support the continued investigation of natural damage-released alarm cue and predator-based repellents for the behavioural manipulation of P. marinus populations in the Laurentian Great Lakes. © 2014 The Fisheries Society of the British Isles.

  1. Characterization of Metarhizium viride Mycosis in Veiled Chameleons (Chamaeleo calyptratus), Panther Chameleons (Furcifer pardalis), and Inland Bearded Dragons (Pogona vitticeps).

    PubMed

    Schmidt, Volker; Klasen, Linus; Schneider, Juliane; Hübel, Jens; Pees, Michael

    2017-03-01

    Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with clinical signs and pathological findings as well as improvement of diagnostic procedures. Beside 18S ribosomal DNA (rDNA) (small subunit [SSU]) and internal transcribed spacer region 1 (ITS-1), a fragment of the large subunit (LSU) of 28S rDNA, including domain 1 (D1) and D2, was sequenced for the identification of the fungus and phylogenetic analysis. Cultural isolation and histopathological examinations as well as the pattern of antifungal drug resistance, determined by using agar diffusion testing, were additionally used for comparison of the isolates. In total, 20 isolates from eight inland bearded dragons ( Pogona vitticeps ), six veiled chameleons ( Chamaeleo calyptratus ), and six panther chameleons ( Furcifer pardalis ) were examined. Most of the lizards suffered from fungal glossitis, stomatitis, and pharyngitis or died due to visceral mycosis. Treatment with different antifungal drugs according to resistance patterns in all three different lizard species was unsuccessful. Sequence analysis resulted in four different genotypes of M. viride based on differences in the LSU fragment, whereas the SSU and ITS-1 were identical in all isolates. Sequence analysis of the SSU fragment revealed the first presentation of a valid large fragment of the SSU of M. viride According to statistical analysis, genotypes did not correlate with differences in pathogenicity, antifungal susceptibility, or species specificity. Copyright © 2017 American Society for Microbiology.

  2. Characterization of Metarhizium viride Mycosis in Veiled Chameleons (Chamaeleo calyptratus), Panther Chameleons (Furcifer pardalis), and Inland Bearded Dragons (Pogona vitticeps)

    PubMed Central

    Klasen, Linus; Schneider, Juliane; Hübel, Jens; Pees, Michael

    2016-01-01

    ABSTRACT Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with clinical signs and pathological findings as well as improvement of diagnostic procedures. Beside 18S ribosomal DNA (rDNA) (small subunit [SSU]) and internal transcribed spacer region 1 (ITS-1), a fragment of the large subunit (LSU) of 28S rDNA, including domain 1 (D1) and D2, was sequenced for the identification of the fungus and phylogenetic analysis. Cultural isolation and histopathological examinations as well as the pattern of antifungal drug resistance, determined by using agar diffusion testing, were additionally used for comparison of the isolates. In total, 20 isolates from eight inland bearded dragons (Pogona vitticeps), six veiled chameleons (Chamaeleo calyptratus), and six panther chameleons (Furcifer pardalis) were examined. Most of the lizards suffered from fungal glossitis, stomatitis, and pharyngitis or died due to visceral mycosis. Treatment with different antifungal drugs according to resistance patterns in all three different lizard species was unsuccessful. Sequence analysis resulted in four different genotypes of M. viride based on differences in the LSU fragment, whereas the SSU and ITS-1 were identical in all isolates. Sequence analysis of the SSU fragment revealed the first presentation of a valid large fragment of the SSU of M. viride. According to statistical analysis, genotypes did not correlate with differences in pathogenicity, antifungal susceptibility, or species specificity. PMID:28003420

  3. Identification of short single disulfide-containing contryphans from the venom of cone snails using de novo mass spectrometry-based sequencing methods.

    PubMed

    Franklin, Jayaseelan Benjamin; Rajesh, Rajaian Pushpabai; Vinithkumar, Nambali Valsalan; Kirubagaran, Ramalingam

    2017-06-15

    We identified 12 short single disulfide-containing conopeptides from the venom of Conus coronatus, C. leopardus, C. lividus and C. zonatus. Interestingly, we detected the shortest contryphan sequence thus far characterized which contains only six amino acid residues. We also identified three distinct contryphan sequences of C. lividus without any proline residues and one sequence with an unusual post-translational modification (bromination of tryptophan). Furthermore, we characterized venom peptides of C. zonatus for the first time. Copyright © 2017 Elsevier Ltd. All rights reserved.

  4. Clostridium difficile and Clostridium perfringens from wild carnivore species in Brazil.

    PubMed

    Silva, Rodrigo Otávio Silveira; D'Elia, Mirella Lauria; Tostes Teixeira, Erika Procópio; Pereira, Pedro Lúcio Lithg; de Magalhães Soares, Danielle Ferreira; Cavalcanti, Álvaro Roberto; Kocuvan, Aleksander; Rupnik, Maja; Santos, André Luiz Quagliatto; Junior, Carlos Augusto Oliveira; Lobato, Francisco Carlos Faria

    2014-08-01

    Despite some case reports, the importance of Clostridium perfringens and Clostridium difficile for wild carnivores remains unclear. Thus, the objective of this study was to identify C. perfringens and C. difficile strains in stool samples from wild carnivore species in Brazil. A total of 34 stool samples were collected and subjected to C. perfringens and C. difficile isolation. Suggestive colonies of C. perfringens were then analyzed for genes encoding the major C. perfringens toxins (alpha, beta, epsilon and iota) and the beta-2 toxin (cpb2), enterotoxin (cpe) and NetB (netb) genes. C. difficile strains were analyzed by multiplex-PCR for toxins A (tcdA) and B (tcdB) and a binary toxin gene (cdtB) and also submitted to a PCR ribotyping. Unthawed aliquots of samples positive for C. difficile isolation were subjected to the detection of A/B toxins by a cytotoxicity assay (CTA). C. perfringens was isolated from 26 samples (76.5%), all of which were genotyped as type A. The netb gene was not detected, whereas the cpb2 and cpe genes were found in nine and three C. perfringens strains, respectively. C. difficile was isolated from two (5.9%) samples. A non-toxigenic strain was recovered from a non-diarrheic maned wolf (Chrysocyon brachyurus). Conversely, a toxigenic strain was found in the sample of a diarrheic ocelot (Leopardus pardallis); an unthawed stool sample was also positive for A/B toxins by CTA, indicating a diagnosis of C. difficile-associated diarrhea in this animal. The present work suggests that wild carnivore species could carry C. difficile strains and that they could be susceptible to C. difficile infection. Copyright © 2014 Elsevier Ltd. All rights reserved.

  5. Influence of ancient glacial periods on the Andean fauna: the case of the pampas cat (Leopardus colocolo)

    PubMed Central

    Cossíos, Daniel; Lucherini, Mauro; Ruiz-García, Manuel; Angers, Bernard

    2009-01-01

    Background While numerous studies revealed the major role of environmental changes of the Quaternary on the evolution of biodiversity, research on the influence of that period on current South-American fauna is scarce and have usually focused on lowland regions. In this study, the genetic structure of the pampas cat (Leopardus colocolo), a widely distributed felid, was determined and linked to ancient climate fluctuations on the Andean region. Results Using both mitochondrial sequences and nuclear microsatellites, we inferred the existence of at least four groups of populations in the central Andes, while other three localities, with little sample sizes (n = 3), presented differences in only one of these markers. The distribution of these groups is correlated to latitude, with a central area characterized by admixture of numerous mitochondrial clades. This suggests colonization from at least three glacial refuges and a contact zone between 20 degrees and 23 degrees S following a glaciation event. The similar coalescence times of the mitochondrial haplotypes indicated that the major clades split approximately one million years ago, likely during the Pre-Pastonian glacial period (0.80 – 1.30 MYA), followed by a demographic expansion in every clade during the Aftonian interglacial period (0.45 – 0.62 MYA). Interestingly, this structure roughly corresponds to the current recognised distribution of morphological subspecies. Conclusion The four groups of populations identified here must be considered different management units, and we propose the three localities showing differences in only mtDNA or ncDNA as provisional management units. The results revealed the influence of ancient climate fluctuations on the evolutionary history of this species. It is expected that the other species of land vertebrates with a smaller or similar mobility have been affected in the same manner by the glacial and interglacial periods in the central Andes PMID:19331650

  6. Discovery of South American suckermouth armored catfishes (Loricariidae, Pterygoplichthys spp.) in the Santa Fe River drainage, Suwannee River basin, USA

    USGS Publications Warehouse

    Nico, Leo G.; Butt, Peter L.; Johnston, Gerald R.; Jelks, Howard L.; Kail, Matthew; Walsh, Stephen J.

    2012-01-01

    We report on the occurrence of South American suckermouth armored catfishes (Loricariidae) in the Suwannee River basin, southeastern USA. Over the past few years (2009-2012), loricariid catfishes have been observed at various sites in the Santa Fe River drainage, a major tributary of the Suwannee in the state of Florida. Similar to other introduced populations of Pterygoplichthys, there is high likelihood of hybridization. To date, we have captured nine specimens (270-585 mm, standard length) in the Santa Fe River drainage. One specimen taken from Poe Spring best agrees with Pterygoplichthys gibbiceps (Kner, 1854) or may be a hybrid with either P. pardalis or P. disjunctivus. The other specimens were taken from several sites in the drainage and include seven that best agree with Pterygoplichthys disjunctivus (Weber, 1991); and one a possible P. disjunctivus x P. pardalis hybrid. We observed additional individuals, either these or similar appearing loricariids, in Hornsby and Poe springs and at various sites upstream and downstream of the long (> 4 km) subterranean portion of the Santa Fe River. These specimens represent the first confirmed records of Pterygoplichthys in the Suwannee River basin. The P. gibbiceps specimen represents the first documented record of an adult or near adult of this species in open waters of North America. Pterygoplichthys disjunctivus or its hybrids (perhaps hybrid swarms) are already abundant and widespread in other parts of peninsular Florida, but the Santa Fe River represents a northern extension of the catfish in the state. Pterygoplichthys are still relatively uncommon in the Santa Fe drainage and successful reproduction not yet documented. However, in May 2012 we captured five adult catfish (two mature or maturing males and three gravid females) from a single riverine swallet pool. One male was stationed at a nest burrow (no eggs present). To survive the occasional harsh Florida winters, these South American catfish apparently use artesian springs as thermal refugia. In the Santa Fe River, eradication might be possible during cold periods when catfish congregate in spring habitats. However, should Pterygoplichthys increase in number and disperse more widely, the opportunity to eliminate them from the drainage will pass.

  7. Diet-tissue discrimination factors and turnover of carbon and nitrogen stable isotopes in tissues of an adult predatory coral reef fish, Plectropomus leopardus.

    PubMed

    Matley, J K; Fisk, A T; Tobin, A J; Heupel, M R; Simpfendorfer, C A

    2016-01-15

    Stable isotope ratios (δ(13)C and δ(15)N values) provide a unique perspective into the ecology of animals because the isotope ratio values of consumers reflect the values in food. Despite the value of stable isotopes in ecological studies, the lack of species-specific experimentally derived diet-tissue discrimination factors (DTDFs) and turnover rates limits their application at a broad scale. Furthermore, most aquatic feeding experiments use temperate, fast-growing fish species and few have considered medium- to large-sized adults with low growth rates from tropical ecosystems. A controlled-diet stable isotope feeding trial was conducted over a 196-day period for the adult predatory reef fish leopard coralgrouper (Plectropomus leopardus). This study calculated δ(13)C and δ(15)N DTDFs and turnover rates in five tissues (liver, plasma, red blood cells (RBC), fin, and muscle) using a continuous flow isotope ratio mass spectrometer equipped with an elemental analyzer. In addition, the effect of chemical lipid extraction (LE) on stable isotope values was examined for each tissue. Turnover was mainly influenced by metabolism (as opposed to growth) with LE δ(15)N half-life values lowest in fin (37 days) and plasma (66 days), and highest in RBC (88 days) and muscle (126 days). The diet-tissue discrimination factors for δ(15)N values in all tissues (Δ(15)N: -0.15 to 1.84‰) were typically lower than commonly reported literature values. Lipid extraction altered both δ(15) N and δ(13)C values compared with untreated samples; however, for the δ(15)N values, the differences were small (mean δ(15)N(LE-Bulk) <0.46‰ in all tissues). This study informs future interpretation of stable isotope data for medium- to large-sized fish and demonstrates that DTDFs developed for temperate fish species, particularly for δ(15)N values, may not apply to tropical species. Sampling of muscle and/or RBC is recommended for a relatively long-term representation of feeding habits, while plasma and/or fin should be used for a more recent indication of diet. Copyright © 2015 John Wiley & Sons, Ltd.

  8. The use of altrenogest to avoid hyperestrogenism after eCG-hCG ovulation induction in southern tigrina (Leopardus guttulus).

    PubMed

    Micheletti, Tatiane; Brown, Janine L; Walker, Susan L; Cubas, Zalmir S; Furtado, Priscila V; Putman, Sarah B; de Moraes, Wanderlei; de Oliveira, Marcos J; de Oliveira, Claudio A; Moreira, Nei

    2015-09-01

    The goal of this study was to optimize an ovulation induction protocol for use with artificial insemination in the southern tigrina (Leopardus guttulus). The specific aims were to report the efficacy of using altrenogest, an oral progestin (Regumate, MSD Animal Health, Merck Animal Health), to suppress ovarian activity and prevent follicular hyperstimulation and hyperestrogenism after the administration of exogenous eCG and hCG. To monitor ovarian responses, fecal estrogen and progestogen metabolites were quantified by enzyme immunoassay in females before and after intramuscular administration of 200-IU eCG and 150-IU hCG in two trials, 4 months apart. During the first trial, there was no use of altrenogest, only the eCG-hCG ovulation induction protocol. In the second trial, the ovulation induction protocol was preceded by the administration of oral altrenogest for 14 days (minimum of 0.192 mg per kg per day). Altrenogest administration resulted in a suppression of follicular activity in three out of six females before eCG-hCG administration on the basis of lower mean estrogen concentrations (P < 0.05). It also resulted in four out of six females presenting lower fecal estrogen metabolite concentrations (P < 0.05) after ovulation induction, and two out of six individuals showed a reduction (P < 0.05) in postovulatory fecal progestogen metabolite concentrations, all when compared to the same female's cycles without the progestin. Fecal estrogen metabolite concentrations were closer to baseline in 50% of these individuals after altrenogest and eCG-hCG treatments when compared to basal concentrations before gonadotropins without the use of altrenogest. This study reported that use of altrenogest in southern tigrina can suppress ovarian activity and avoid hyperestrogenism after administration of eCG and hCG treatment. However, not all females responded uniformly, so more studies are needed to increase the efficacy of ovulation induction for use with artificial insemination in this species. Copyright © 2015 Elsevier Inc. All rights reserved.

  9. Molecular data reveal complex hybridization and a cryptic species of neotropical wild cat.

    PubMed

    Trigo, Tatiane C; Schneider, Alexsandra; de Oliveira, Tadeu G; Lehugeur, Livia M; Silveira, Leandro; Freitas, Thales R O; Eizirik, Eduardo

    2013-12-16

    Hybridization among animal species has recently become more recognized as an important phenomenon, especially in the context of recent radiations. Here we show that complex hybridization has led to contrasting patterns of genomic composition among closely related species of the Neotropical cat genus Leopardus. We show strong evidence of ancient hybridization and introgression between the pampas cat (L. colocolo) and northeastern populations of tigrina (L. tigrinus), leading to remarkable cytonuclear discordance in the latter. In contrast, southern tigrina populations show recent and continuing hybridization with Geoffroy's cat (L. geoffroyi), leading to extreme levels of interspecific admixture at their contact zone. Finally, we demonstrate that two seemingly continuous Brazilian tigrina populations show no evidence of ongoing gene flow between them, leading us to support their formal recognition as distinct species, namely L. tigrinus in the northeast and L. guttulus in the south. Copyright © 2013 Elsevier Ltd. All rights reserved.

  10. A spatially integrated framework for assessing socioecological drivers of carnivore decline.

    PubMed

    Gálvez, Nicolás; Guillera-Arroita, Gurutzeta; St John, Freya A V; Schüttler, Elke; Macdonald, David W; Davies, Zoe G

    2018-05-01

    Habitat loss, fragmentation and degradation are key threats to the long-term persistence of carnivores, which are also susceptible to direct persecution by people. Integrating natural and social science methods to examine how habitat configuration/quality and human-predator relations may interact in space and time to effect carnivore populations within human-dominated landscapes will help prioritise conservation investment and action effectively.We propose a socioecological modelling framework to evaluate drivers of carnivore decline in landscapes where predators and people coexist. By collecting social and ecological data at the same spatial scale, candidate models can be used to quantify and tease apart the relative importance of different threats.We apply our methodological framework to an empirical case study, the threatened güiña ( Leopardus guigna ) in the temperate forest ecoregion of southern Chile, to illustrate its use. Existing literature suggests that the species is declining due to habitat loss, fragmentation and persecution in response to livestock predation. Data used in modelling were derived from four seasons of camera-trap surveys, remote-sensed images and household questionnaires.Occupancy dynamics were explained by habitat configuration/quality covariates rather than by human-predator relations. Güiñas can tolerate a high degree of habitat loss (>80% within a home range). They are primarily impacted by fragmentation and land subdivision (larger farms being divided into smaller ones). Ten per cent of surveyed farmers ( N  = 233) reported illegally killing the species over the past decade. Synthesis and applications . By integrating ecological and social data, collected at the same spatial scale, within a single modelling framework, our study demonstrates the value of an interdisciplinary approach to assessing the potential threats to a carnivore. It has allowed us to tease apart effectively the relative importance of different potential extinction pressures for the güiña ( Leopardus guigna ), make informed conservation recommendations and prioritise where future interventions should be targeted. We have identified that human-dominated landscapes with large intensive farms can be of conservation value, as long as an appropriate network of habitat patches is maintained within the matrix. Conservation efforts to secure the long-term persistence of the species should focus on reducing habitat fragmentation rather than human persecution.

  11. Late Pleistocene carnivores (Carnivora: Mammalia) from a cave sedimentary deposit in northern Brazil.

    PubMed

    Rodrigues, Shirlley; Avilla, Leonardo S; Soibelzon, Leopoldo H; Bernardes, Camila

    2014-12-01

    The Brazilian Quaternary terrestrial Carnivora are represented by the following families: Canidae, Felidae, Ursidae, Procyonidae Mephitidae and Mustelidae. Their recent evolutionary history in South America is associated with the uplift of the Panamanian Isthmus, and which enabled the Great American Biotic Interchange (GABI). Here we present new fossil records of Carnivora found in a cave in Aurora do Tocantins, Tocantins, northern Brazil. A stratigraphical controlled collection in the sedimentary deposit of the studied cave revealed a fossiliferous level where the following Carnivora taxa were present: Panthera onca, Leopardus sp., Galictis cuja, Procyon cancrivorus, Nasua nasua and Arctotherium wingei. Dating by Electron Spinning Resonance indicates that this assemblage was deposited during the Last Glacial Maximum (LGM), at least, 22.000 YBP. The weasel, G. cuja, is currently reported much further south than the record presented here. This may suggest that the environment around the cave was relatively drier during the LGM, with more open vegetation, and more moderate temperatures than the current Brazilian Cerrado.

  12. Late Pleistocene carnivores (Carnivora: Mammalia) from a cave sedimentary deposit in northern Brazil.

    PubMed

    Rodrigues, Shirlley; Avilla, Leonardo S; Soibelzon, Leopoldo H; Bernardes, Camila

    2014-11-28

    The Brazilian Quaternary terrestrial Carnivora are represented by the following families: Canidae, Felidae, Ursidae, Procyonidae Mephitidae and Mustelidae. Their recent evolutionary history in South America is associated with the uplift of the Panamanian Isthmus, and which enabled the Great American Biotic Interchange (GABI). Here we present new fossil records of Carnivora found in a cave in Aurora do Tocantins, Tocantins, northern Brazil. A stratigraphical controlled collection in the sedimentary deposit of the studied cave revealed a fossiliferous level where the following Carnivora taxa were present: Panthera onca, Leopardus sp., Galictis cuja, Procyon cancrivorus, Nasua nasua and Arctotherium wingei. Dating by Electron Spinning Resonance indicates that this assemblage was deposited during the Last Glacial Maximum (LGM), at least, 22.000 YBP. The weasel, G. cuja, is currently reported much further south than the record presented here. This may suggest that the environment around the cave was relatively drier during the LGM, with more open vegetation, and more moderate temperatures than the current Brazilian Cerrado.

  13. Large predatory coral trout species unlikely to meet increasing energetic demands in a warming ocean.

    PubMed

    Johansen, J L; Pratchett, M S; Messmer, V; Coker, D J; Tobin, A J; Hoey, A S

    2015-09-08

    Increased ocean temperature due to climate change is raising metabolic demands and energy requirements of marine ectotherms. If productivity of marine systems and fisheries are to persist, individual species must compensate for this demand through increasing energy acquisition or decreasing energy expenditure. Here we reveal that the most important coral reef fishery species in the Indo-west Pacific, the large predatory coral trout Plectropomus leopardus (Serranidae), can behaviourally adjust food intake to maintain body-condition under elevated temperatures, and acclimate over time to consume larger meals. However, these increased energetic demands are unlikely to be met by adequate production at lower trophic levels, as smaller prey species are often the first to decline in response to climate-induced loss of live coral and structural complexity. Consequently, ubiquitous increases in energy consumption due to climate change will increase top-down competition for a dwindling biomass of prey, potentially distorting entire food webs and associated fisheries.

  14. Large predatory coral trout species unlikely to meet increasing energetic demands in a warming ocean

    PubMed Central

    Johansen, J.L.; Pratchett, M.S.; Messmer, V.; Coker, D.J.; Tobin, A.J.; Hoey, A.S.

    2015-01-01

    Increased ocean temperature due to climate change is raising metabolic demands and energy requirements of marine ectotherms. If productivity of marine systems and fisheries are to persist, individual species must compensate for this demand through increasing energy acquisition or decreasing energy expenditure. Here we reveal that the most important coral reef fishery species in the Indo-west Pacific, the large predatory coral trout Plectropomus leopardus (Serranidae), can behaviourally adjust food intake to maintain body-condition under elevated temperatures, and acclimate over time to consume larger meals. However, these increased energetic demands are unlikely to be met by adequate production at lower trophic levels, as smaller prey species are often the first to decline in response to climate-induced loss of live coral and structural complexity. Consequently, ubiquitous increases in energy consumption due to climate change will increase top-down competition for a dwindling biomass of prey, potentially distorting entire food webs and associated fisheries. PMID:26345733

  15. Taxonomy of Cotylea (Platyhelminthes: Polycladida) from Cabo Frio, southeastern Brazil, with the description of a new species.

    PubMed

    Bahia, Juliana; Padula, Vinicius; Lavrado, Helena Passeri; Quiroga, Sigmer

    2014-10-20

    Polyclads are free-living Platyhelminthes with a simple, dorsoventrally flattened body and a much ramified intestine. In Brazil, 66 species are reported; only three from Rio de Janeiro State (RJ). The main objective of this study is to describe and illustrate coloration pattern, external morphology, reproductive system morphology and, when possible, biological and ecological aspects of species of the suborder Cotylea found in Cabo Frio, RJ. Of the 13 cotylean polyclad species found, Pseudobiceros pardalis, Cycloporus variegatus and Eurylepta aurantiaca are new records from the Brazilian coast and one species is new to science, Pseudoceros juani sp. nov. Feeding observations were made of four species. It is the first time that Lurymare utarum, Cycloporus gabriellae, C. variegatus and E. aurantiaca are illustrated with digital photographs of live specimens and histological preparations. This study increases to 70 the number of Brazilian Polycladida and to 14 the number of species known from Rio de Janeiro State. However, the knowledge about Polycladida in Brazil still has gaps, with great parts of the coast remaining unsampled. 

  16. Taxonomic revision of the South American catfish genus Ageneiosus (Siluriformes: Auchenipteridae) with the description of four new species

    USGS Publications Warehouse

    Ribeiro, Frank R.V.; Rapp Py-Daniel, Lúcia H.; Walsh, Stephen J.

    2017-01-01

    The catfish genus Ageneiosus in the exclusively Neotropical family Auchenipteridae is revised. Species of Ageneiosus are widely distributed in all major South American continental drainages except the São Francisco River basin and small rivers along the Brazilian east coast. The taxonomic revision was based on examination of available type specimens, additional museum material and comparisons of original descriptions. A suite of morphometric, meristic and qualitative characters of internal and external anatomy were used to diagnose valid species and determine synonyms. Thirteen valid species are recognized in the genus Ageneiosus, some of which are widely distributed across South America. Ageneiosus pardalis is the only trans-Andean species in the genus. Ageneiosus polystictus and Ageneiosus uranophthalmus are more widely distributed than previously reported. Ageneiosus marmoratus is a junior synonym of Ageneiosus inermis. Ageneiosus dentatus is a valid species and its name is removed from the synonymy of Ageneiosus ucayalensis. Four new species are described: Ageneiosus akamai, Ageneiosus apiaka, Ageneiosus intrusus and Ageneiosus lineatus, all from the Amazon River basin. A dichotomous key for all 13 valid species of Ageneiosus species is provided.

  17. Serologic survey of domestic felids in the Petén region of Guatemala.

    PubMed

    Lickey, Adrienne L A; Kennedy, Melissa; Patton, Sharon; Ramsay, Edward C

    2005-03-01

    Blood samples were analyzed from 30 domestic cats (Felis domesticus) from the Petén region of Guatemala to determine the seroprevalence of common pathogens that may pose a potential risk to native wild felids. Eight of the cats had been vaccinated previously; however, owners were unable to fully describe the type of vaccine and date of administration. In addition, blood samples were obtained from two captive margays (Leopardus wiedii). Samples were tested for antibodies to feline immunodeficiency virus, Dirofilaria immitis, feline panleukopenia virus, feline herpesvirus, feline coronavirus, canine distemper virus, and Toxoplasma gondii and for feline leukemia virus (FeLV) antigen. Fifty percent or more of the cats sampled were seropositive for feline herpesvirus (22 of 30), feline panleukopenia (15 of 30), and T. gondii (16 of 30). Five cats were positive for FeLV antigen. Both margays were seropositive for feline coronavirus and one was strongly seropositive to T. gondii. All animals were seronegative for D. immitis. This survey provides preliminary information about feline diseases endemic to the Petén region.

  18. Detection and phylogenetic analysis of a new adenoviral polymerase gene in reptiles in Korea.

    PubMed

    Bak, Eun-Jung; Jho, Yeonsook; Woo, Gye-Hyeong

    2018-06-01

    Over a period of 7 years (2004-2011), samples from 34 diseased reptiles provided by local governments, zoos, and pet shops were tested for viral infection. Animals were diagnosed based on clinical signs, including loss of appetite, diarrhea, rhinorrhea, and unexpected sudden death. Most of the exotic animals had gastrointestinal problems, such as mucosal redness and ulcers, while the native animals had no clinical symptoms. Viral sequences were found in seven animals. Retroviral genes were amplified from samples from five Burmese pythons (Python molurus bivittatus), an adenovirus was detected in a panther chameleon (Furcifer pardalis), and an adenovirus and a paramyxovirus were detected in a tropical girdled lizard (Cordylus tropidosternum). Phylogenetic analysis of retroviruses and paramyxoviruses showed the highest sequence identity to both a Python molurus endogenous retrovirus and a Python curtus endogenous retrovirus and to a lizard isolate, respectively. Partial sequencing of an adenoviral DNA polymerase gene from the lizard isolate suggested that the corresponding virus was a novel isolate different from the reference strain (accession no. AY576677.1). The virus was not isolated but was detected, using molecular genetic techniques, in a lizard raised in a pet shop. This animal was also coinfected with a paramyxovirus.

  19. Ultrasonographic anatomy of the healthy southern tigrina ( Leopardus guttulus) abdomen: comparison with domestic cat references.

    PubMed

    Müller, Thiago R; Marcelino, Raquel S; de Souza, Livia P; Teixeira, Carlos R; Mamprim, Maria J

    2017-02-01

    Objectives The aim of the study was to describe the normal abdominal echoanatomy of the tigrina and to compare it with the abdominal echoanatomy of the domestic cat. Reference intervals for the normal abdominal ultrasonographic anatomy of individual species are important for accurate diagnoses and interpretation of routine health examinations. The hypothesis was that the echoanatomy of the tigrina was similar to that of the domestic cat. Methods Eighteen clinically healthy tigrina were selected for abdominal ultrasound examination, in order to obtain normal parameters of the bladder, spleen, adrenal gland, kidney, gastrointestinal tract, liver and gall bladder, and Doppler parameters of liver and kidney vessels. Results The splenic parenchyma was consistently hyperechoic to the kidneys and liver. The liver, kidneys and spleen had similar echotexture, shape and dimensions when compared with the domestic cat. The gall bladder was lobulated and surrounded by a clearly visualized thin, smooth, regular echogenic wall. The adrenal glands had a bilobulated shape. The urinary bladder had a thin echogenic wall. The Doppler parameters of the portal vein and renal artery were similar to the domestic cat. Conclusions and relevance The results support the hypothesis that the ultrasonographic parameters of the abdominal viscera of the southern tigrina are similar to those of the domestic cat.

  20. Molecular detection of viral agents in free-ranging and captive neotropical felids in Brazil.

    PubMed

    Furtado, Mariana M; Taniwaki, Sueli A; de Barros, Iracema N; Brandão, Paulo E; Catão-Dias, José L; Cavalcanti, Sandra; Cullen, Laury; Filoni, Claudia; Jácomo, Anah T de Almeida; Jorge, Rodrigo S P; Silva, Nairléia Dos Santos; Silveira, Leandro; Ferreira Neto, José S

    2017-09-01

    We describe molecular testing for felid alphaherpesvirus 1 (FHV-1), carnivore protoparvovirus 1 (CPPV-1), feline calicivirus (FCV), alphacoronavirus 1 (feline coronavirus [FCoV]), feline leukemia virus (FeLV), feline immunodeficiency virus (FIV), and canine distemper virus (CDV) in whole blood samples of 109 free-ranging and 68 captive neotropical felids from Brazil. Samples from 2 jaguars ( Panthera onca) and 1 oncilla ( Leopardus tigrinus) were positive for FHV-1; 2 jaguars, 1 puma ( Puma concolor), and 1 jaguarundi ( Herpairulus yagouaroundi) tested positive for CPPV-1; and 1 puma was positive for FIV. Based on comparison of 103 nucleotides of the UL24-UL25 gene, the FHV-1 sequences were 99-100% similar to the FHV-1 strain of domestic cats. Nucleotide sequences of CPPV-1 were closely related to sequences detected in other wild carnivores, comparing 294 nucleotides of the VP1 gene. The FIV nucleotide sequence detected in the free-ranging puma, based on comparison of 444 nucleotides of the pol gene, grouped with other lentiviruses described in pumas, and had 82.4% identity with a free-ranging puma from Yellowstone Park and 79.5% with a captive puma from Brazil. Our data document the circulation of FHV-1, CPPV-1, and FIV in neotropical felids in Brazil.

  1. Natural infection by endoparasites among free-living wild animals.

    PubMed

    Holsback, Luciane; Cardoso, Mauro José Lahm; Fagnani, Rafael; Patelli, Thaís Helena Constantino

    2013-01-01

    The objective of this study was to investigate the frequency of occurrence and variety of intestinal parasites among free-living wild animals. Fecal samples from wild mammals and birds at rehabilitation centers in the states of Mato Grosso do Sul and São Paulo were analyzed by sedimentation and flotation-centrifugation methods. Parasite eggs, oocysts, cysts and/or trophozoites were found in 71% of the samples. Cryptosporidium sp. oocysts were detected in fecal samples from oncillas (Leopardus tigrinus) and scaly-headed parrots (Pionus maximiliani). Giardia cysts were identified in the feces of a gray brocket (Mazama gouazoubira). Among the most common parasites found, there were eggs from Toxocara cati, Toxascaris leonina and Ancylostoma tubaeforme, and from Cestoda. Several Enterobius sp. eggs were found in the feces of red howler monkeys (Alouatta seniculus). It can be concluded from this study that despite the small number of samples, the diversity of parasites found was noteworthy. Additional information about parasite endofauna in wild animals is needed, since their presence might suggest that there could be proximity to and interactions with domestic animals and/or humans. In addition, further studies on parasites from free-living wild animals are of prime importance for understanding the intensity of anthropic changes in wild environments.

  2. Spatially explicit inference for open populations: estimating demographic parameters from camera-trap studies

    USGS Publications Warehouse

    Gardner, Beth; Reppucci, Juan; Lucherini, Mauro; Royle, J. Andrew

    2010-01-01

    We develop a hierarchical capture–recapture model for demographically open populations when auxiliary spatial information about location of capture is obtained. Such spatial capture–recapture data arise from studies based on camera trapping, DNA sampling, and other situations in which a spatial array of devices records encounters of unique individuals. We integrate an individual-based formulation of a Jolly-Seber type model with recently developed spatially explicit capture–recapture models to estimate density and demographic parameters for survival and recruitment. We adopt a Bayesian framework for inference under this model using the method of data augmentation which is implemented in the software program WinBUGS. The model was motivated by a camera trapping study of Pampas cats Leopardus colocolo from Argentina, which we present as an illustration of the model in this paper. We provide estimates of density and the first quantitative assessment of vital rates for the Pampas cat in the High Andes. The precision of these estimates is poor due likely to the sparse data set. Unlike conventional inference methods which usually rely on asymptotic arguments, Bayesian inferences are valid in arbitrary sample sizes, and thus the method is ideal for the study of rare or endangered species for which small data sets are typical.

  3. Spatially explicit inference for open populations: estimating demographic parameters from camera-trap studies.

    PubMed

    Gardner, Beth; Reppucci, Juan; Lucherini, Mauro; Royle, J Andrew

    2010-11-01

    We develop a hierarchical capture-recapture model for demographically open populations when auxiliary spatial information about location of capture is obtained. Such spatial capture-recapture data arise from studies based on camera trapping, DNA sampling, and other situations in which a spatial array of devices records encounters of unique individuals. We integrate an individual-based formulation of a Jolly-Seber type model with recently developed spatially explicit capture-recapture models to estimate density and demographic parameters for survival and recruitment. We adopt a Bayesian framework for inference under this model using the method of data augmentation which is implemented in the software program WinBUGS. The model was motivated by a camera trapping study of Pampas cats Leopardus colocolo from Argentina, which we present as an illustration of the model in this paper. We provide estimates of density and the first quantitative assessment of vital rates for the Pampas cat in the High Andes. The precision of these estimates is poor due likely to the sparse data set. Unlike conventional inference methods which usually rely on asymptotic arguments, Bayesian inferences are valid in arbitrary sample sizes, and thus the method is ideal for the study of rare or endangered species for which small data sets are typical.

  4. Phylogeographic analyses of the pampas cat (Leopardus colocola; Carnivora, Felidae) reveal a complex demographic history

    PubMed Central

    da Silva Santos, Anelisie; Trigo, Tatiane Campos; de Oliveira, Tadeu Gomes; Silveira, Leandro

    2018-01-01

    Abstract The pampas cat is a small felid that occurs in open habitats throughout much of South America. Previous studies have revealed intriguing patterns of morphological differentiation and genetic structure among its populations, as well as molecular evidence for hybridization with the closely related L. tigrinus. Here we report phylogeographic analyses encompassing most of its distribution (focusing particularly on Brazilian specimens, which had been poorly sampled in previous studies), using a novel dataset comprising 2,143 bp of the mitogenome, along with previously reported mtDNA sequences. Our data revealed strong population strutucture and supported a west-to-east colonization process in this species’ history. We detected two population expansion events, one older (ca. 200 thousand years ago [kya]) in western South America and another more recent (ca. 60-50 kya) in eastern areas, coinciding with the expansion of savanna environments in Brazil. Analyses including L. tigrinus individuals bearing introgressed mtDNA from L. colocola showed a complete lack of shared haplotypes between species, indicating that their hybridization was ancient. Finally, we observed a close relationship between Brazilian/Uruguayan L. colocola haplotypes and those sampled in L. tigrinus, indicating that their hybridization was likely related to the demographic expansion of L. colocola into eastern South America. PMID:29668017

  5. Diagnosing Tic Disorders

    MedlinePlus

    ... span class="tp-sr-only">Submit Button Submit Button Tourette Syndrome (TS): Risk Factors and Causes

    MedlinePlus

    ... span class="tp-sr-only">Submit Button Submit Button Female heterogamety in Madagascar chameleons (Squamata: Chamaeleonidae: Furcifer): differentiation of sex and neo-sex chromosomes

    PubMed Central

    Rovatsos, Michail; Pokorná, Martina Johnson; Altmanová, Marie; Kratochvíl, Lukáš

    2015-01-01

    Amniotes possess variability in sex determining mechanisms, however, this diversity is still only partially known throughout the clade and sex determining systems still remain unknown even in such a popular and distinctive lineage as chameleons (Squamata: Acrodonta: Chamaeleonidae). Here, we present evidence for female heterogamety in this group. The Malagasy giant chameleon (Furcifer oustaleti) (chromosome number 2n = 22) possesses heteromorphic Z and W sex chromosomes with heterochromatic W. The panther chameleon (Furcifer pardalis) (2n = 22 in males, 21 in females), the second most popular chameleon species in the world pet trade, exhibits a rather rare Z1Z1Z2Z2/Z1Z2W system of multiple sex chromosomes, which most likely evolved from W-autosome fusion. Notably, its neo-W chromosome is partially heterochromatic and its female-specific genetic content has expanded into the previously autosomal region. Showing clear evidence for genotypic sex determination in the panther chameleon, we resolve the long-standing question of whether or not environmental sex determination exists in this species. Together with recent findings in other reptile lineages, our work demonstrates that female heterogamety is widespread among amniotes, adding another important piece to the mosaic of knowledge on sex determination in amniotes needed to understand the evolution of this important trait. PMID:26286647

  6. Experimental infection of several fish species with the causative agent of Kuchijirosho (snout ulcer disease) derived from the tiger puffer Takifugu rubripes.

    PubMed

    Miyadai, T; Kitamura, S I; Uwaoku, H; Tahara, D

    2001-12-05

    Kuchijirosho (snout ulcer disease) is a fatal epidemic disease which affects the tiger puffer, Takifugu rubripes, a commercial fish species in Japan and Korea. To assess the possibility that non-tiger puffer fish can serve as reservoirs of infection, 5 fish species were challenged by infection with the extracts of Kuchijirosho-affected brains from cultured tiger puffer: grass puffer T. niphobles, fine-patterned puffer T. poecilonotus, panther puffer T. pardalis, red sea bream Pagrus major, and black rockfish Sebastes schlegeli. When slightly irritated, all these species, especially the puffer fish, exhibited typical signs of Kuchijirosho, i.e., erratic swimming, biting together and bellying out (swelling of belly), as generally observed in tiger puffers affected by Kuchijirosho. Although the mortalities of the 2 non-puffer species were lower, injection of the extracts prepared from the brains of both inoculated fish into tiger puffer resulted in death, indicating that the inoculated fish used in this experiment have the potential to be infected with the Kuchijirosho agent. Condensations of nuclei or chromatin in the large nerve cells, which is a major characteristic of Kuchijirosho, were histopathologically observed to some extent in the brains of all kinds of puffer fish species infected. These findings suggest that the virus can spread horizontally among wild and cultured puffers and even among fishes belonging to different orders.

  7. Digestive parameters and water turnover of the leopard tortoise.

    PubMed

    McMaster, Megan K; Downs, Colleen T

    2008-09-01

    Leopard tortoises (Stigmochelys pardalis) experience wide fluctuations in environmental conditions and unpredictable availability of food and water within the Nama-Karoo biome. It was hypothesised that tortoises fed two diets differing in preformed water and fibre content would have differing food intake, gut transit rate, assimilation efficiency, faecal and urinary water loss, and urine concentrations. It was predicted that tortoises fed these contrasting diets would attempt to maintain energy and water balance by altering their digestive parameters. Leopard tortoises fed lucerne (Medicago sativa) had a low food intake coupled with long gut transit times, which resulted in the lowest amount of faecal energy and faecal water lost. Tortoises fed tomatoes (Solanum lycopersicum) had higher food intake and faster gut transit times, but more energy and water was lost in the faeces. However, daily energy assimilated and assimilation efficiency were comparable between tortoises fed the two diets. Urine osmolality was significantly different between tortoises on the two diets. Results indicate that leopard tortoises can adjust parameters such as transit rate, food intake, water loss and urine osmolality to maintain body mass, water and energy balance in response to a high fibre, low water content and a low fibre, high water content diet. This study suggests that this digestive flexibility allows leopard tortoises in the wild to take advantage of unpredictable food and water resources.

  8. Rising temperatures may drive fishing-induced selection of low-performance phenotypes

    PubMed Central

    Clark, Timothy D.; Messmer, Vanessa; Tobin, Andrew J.; Hoey, Andrew S.; Pratchett, Morgan S.

    2017-01-01

    Climate warming is likely to interact with other stressors to challenge the physiological capacities and survival of phenotypes within populations. This may be especially true for the billions of fishes per year that undergo vigorous exercise prior to escaping or being intentionally released from fishing gear. Using adult coral grouper (Plectropomus leopardus), an important fisheries species throughout the Indo-Pacific, we show that population-level survival following vigorous exercise is increasingly compromised as temperatures increase from current-day levels (100–67% survival at 24–30 °C) to those projected for the end of the century (42% survival at 33 °C). Intriguingly, we demonstrate that high-performance individuals take longer to recover to a resting metabolic state and subsequently have lower survival in warm water compared with conspecifics that exercise less vigorously. Moreover, we show that post-exercise mortality of high-performance phenotypes manifests after 3–13 d at the current summer maximum (30 °C), while mortality at 33 °C occurs within 1.8–14.9 h. We propose that wild populations in a warming climate may become skewed towards low-performance phenotypes with ramifications for predator-prey interactions and community dynamics. Our findings highlight the susceptibility of phenotypic diversity to fishing activities and demonstrate a mechanism that may contribute to fishing-induced evolution in the face of ongoing climate change. PMID:28094310

  9. Values, animal symbolism, and human-animal relationships associated to two threatened felids in Mapuche and Chilean local narratives.

    PubMed

    Herrmann, Thora M; Schüttler, Elke; Benavides, Pelayo; Gálvez, Nicolas; Söhn, Lisa; Palomo, Nadja

    2013-06-13

    The Chilean temperate rainforest has been subjected to dramatic fragmentation for agriculture and forestry exploitation. Carnivore species are particularly affected by fragmentation and the resulting resource use conflicts with humans. This study aimed at understanding values and human-animal relationships with negatively perceived threatened carnivores through the disclosure of local stories and Mapuche traditional folktales. Our mixed approach comprised the qualitative analysis of 112 stories on the kodkod cat (Leopardus guigna) and the puma (Puma concolor) collected by students (9-14 years) from 28 schools in the Araucania region within their family contexts, 10 qualitative in-depth interviews with indigenous Mapuche people, 35 traditional Mapuche legends, and the significance of naming found in ethnographic collections. We revealed a quasi-extinction of traditional tales in the current knowledge pool about pumas and kodkods, local anecdotes, however, were present in significant numbers. Values associated to both felids were manifold, ranging from negativistic to positive values. While pumas played an important role in people's spirituality, negative mythological connotations persisted in kodkod stories. Four prominent relationships were derived: (1) Both felids represent threats to livestock, pumas even to life, (2) both felids are symbols for upcoming negative events, (3) pumas are spiritual creatures, and (4) kodkods are threatened by humans. Recommendations are provided for stimulating new ways of perceiving unpopular and threatened carnivores among those who live in vicinity to them.

  10. Using DNA Barcodes to Identify Road-Killed Animals in Two Atlantic Forest Nature Reserves, Brazil

    PubMed Central

    Klippel, Angélica H.; Oliveira, Pablo V.; Britto, Karollini B.; Freire, Bárbara F.; Moreno, Marcel R.; dos Santos, Alexandre R.; Banhos, Aureo; Paneto, Greiciane G.

    2015-01-01

    Road mortality is the leading source of biodiversity loss in the world, especially due to fragmentation of natural habitats and loss of wildlife. The survey of the main species victims of roadkill is of fundamental importance for the better understanding of the problem, being necessary, for this, the correct species identification. The aim of this study was to verify if DNA barcodes can be applied to identify road-killed samples that often cannot be determined morphologically. For this purpose, 222 vertebrate samples were collected in a stretch of the BR-101 highway that crosses two Discovery Coast Atlantic Forest Natural Reserves, the Sooretama Biological Reserve and the Vale Natural Reserve, in Espírito Santo, Brazil. The mitochondrial COI gene was amplified, sequenced and confronted with the BOLD database. It was possible to identify 62.16% of samples, totaling 62 different species, including Pyrrhura cruentata, Chaetomys subspinosus, Puma yagouaroundi and Leopardus wiedii considered Vulnerable in the National Official List of Species of Endangered Wildlife. The most commonly identified animals were a bat (Molossus molossus), an opossum (Didelphis aurita) and a frog (Trachycephalus mesophaeus) species. Only one reptile was identified using the technique, probably due to lack of reference sequences in BOLD. These data may contribute to a better understanding of the impact of roads on species biodiversity loss and to introduce the DNA barcode technique to road ecology scenarios. PMID:26244644

  11. Toxoplasma gondii infection in wild mustelids and cats across an urban-rural gradient.

    PubMed

    Barros, Macarena; Cabezón, Oscar; Dubey, Jitender P; Almería, Sonia; Ribas, María P; Escobar, Luis E; Ramos, Barbara; Medina-Vogel, Gonzalo

    2018-01-01

    The increase in human population and domestic pets, such as cats, are generating important consequences in terms of habitat loss and pathogen pollution of coastal ecosystems with potential to generate negative impacts in marine biodiversity. Toxoplasma gondii is the etiological agent of zoonotic disease toxoplasmosis, and is associated with cat abundance and anthropogenic disturbance. The presence of T. gondii oocysts in the ocean has negatively affected the health status of the threatened Southern sea otter (Enhydra lutris nereis) populations. The present study analyzed seroprevalence and presence of T. gondii DNA in American mink (Neovison vison), Southern river otters (Lontra provocax) and domestic cats (Felis silvestris catus) in four different areas in Southern Chile comprising studies in rivers and lakes in Andean foothills and mountains, marine habitat and island coastal ecosystems. Mean seroprevalence of T. gondii in the study was 64% of 151 total animals sampled: 59% of 73 American mink, 77% of 13 Southern river otters, 68% of 65 domestic cats and in two of two kodkods (Leopardus guigna). Toxoplasma gondii DNA was detected in tissues from one American mink and one Southern river otter. The present study confirms the widespread distribution of T. gondii in Southern Chile, and shows a high exposure of semiaquatic mustelids and domestic cats to the parasite. Cats and anthropogenic disturbance have a role in the maintenance of T. gondii infection in ecosystems of southern Chile.

  12. Study of an outbreak of ciguatera fish poisoning in Hong Kong.

    PubMed

    Wong, Chun-Kwan; Hung, Patricia; Lee, Kellie L H; Kam, Kai-Man

    2005-10-01

    Ciguatera fish poisoning (CFP) has been a significant and increasing public health problem in Hong Kong since 1980s. With growing demand for imported live coral fishes, the number of people who suffered from this disease has also been increasing. An outbreak of CFP in 2004 was the second most prominent in record as compared with the most significant one that occurred in 1998. In 2004, out of a total of 823 reported food poisoning outbreaks involving 3159 persons, 65 incidents (7.9%) affecting 247 people (7.8%) were attributed to CFP. Validated mouse bioassay analysis of surveillance samples revealed that seven samples (13%) were confirmed to be contaminated with ciguatoxins (CTXs). Typical symptoms of CTXs were found in mice injected with 20mg of fish extracts. The causative fishes included Cheilinus undulatus, Epinephelus coioides, Plectropomus areolatus, and Plectropomus leopardus. Most of these CTX-positive samples analyzed had only trace amounts of CTXs in their extract, except a C. undulatus sample which contained a mice lethal dose (2.5MU/20mg ether extract). This fish species was also the major origin of coral fish that caused clusters of CFP in the last quarter of 2004. Cigua-Check analysis of 20 flesh grains from seven CTX-positive fishes, previously confirmed as CTX-positive samples by mouse bioassay, showed that 50% of flesh grains were CTX contaminated.

  13. 34 CFR 668.137 - Deadlines for submitting documentation and the consequences of failure to submit documentation.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 3 2010-07-01 2010-07-01 false Deadlines for submitting documentation and the consequences of failure to submit documentation. 668.137 Section 668.137 Education Regulations of the Offices... documentation and the consequences of failure to submit documentation. (a) A student shall submit before a...

  14. 21 CFR 1271.21 - When do I register, submit an HCT/P list, and submit updates?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false When do I register, submit an HCT/P list, and... Listing § 1271.21 When do I register, submit an HCT/P list, and submit updates? (a) You must register and submit a list of every HCT/P that your establishment manufactures within 5 days after beginning...

  15. 21 CFR 1271.21 - When do I register, submit an HCT/P list, and submit updates?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false When do I register, submit an HCT/P list, and... Listing § 1271.21 When do I register, submit an HCT/P list, and submit updates? (a) You must register and submit a list of every HCT/P that your establishment manufactures within 5 days after beginning...

  16. 21 CFR 1271.21 - When do I register, submit an HCT/P list, and submit updates?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false When do I register, submit an HCT/P list, and... Listing § 1271.21 When do I register, submit an HCT/P list, and submit updates? (a) You must register and submit a list of every HCT/P that your establishment manufactures within 5 days after beginning...

  17. How to Submit a Risk Management Plan (RMP) to EPA

    EPA Pesticide Factsheets

    RMP*eSubmit software is the only way to submit RMPs. After you have prepared your plan using RMP*eSubmit, you may also re-submit, correct, or withdraw an RMP. Another electronic tool, RMP*Comp, performs the required off-site consequence analysis.

  18. Serum concentrations of lipids, vitamin d metabolites, retinol, retinyl esters, tocopherols and selected carotenoids in twelve captive wild felid species at four zoos.

    PubMed

    Crissey, Susan D; Ange, Kimberly D; Jacobsen, Krista L; Slifka, Kerri A; Bowen, Phyllis E; Stacewicz-Sapuntzakis, Maria; Langman, Craig B; Sadler, William; Kahn, Stephen; Ward, Ann

    2003-01-01

    Serum concentrations of several nutrients were measured in 12 captive wild felid species including caracal (Felis caracal), cheetah (Acinonyx jubatus), cougar (Felis concolor), fishing cat (Felis viverrinus), leopard (Panthera pardus), lion (Panthera leo), ocelot (Felis pardalis), pallas cat (Felis manul), sand cat (Felis margarita), serval (Felis serval), snow leopard (Panthera uncia) and tiger (Panthera tigris). Diet information was collected for these animals from each participating zoo (Brookfield Zoo, Fort Worth Zoo, Lincoln Park Zoological Gardens and North Carolina Zoological Park). The nutritional composition of the diets at each institution met the probable dietary requirements for each species except for the pallas cat. Blood samples were collected from each animal (n = 69) and analyzed for lipids (total cholesterol, triacylglycerides, HDL cholesterol and LDL cholesterol), vitamin D metabolites [25-hydroxycholecalciferol (25(OH)D) and 1,25-dihydroxycholecalciferol (1,25(OH)(2)D)], vitamin A (retinol, retinyl stearate and retinyl palmitate), vitamin E (alpha- and gamma-tocopherol) and selected carotenoids. Species differences were found for all except triacylglycerides and 1,25(OH)(2)D. Genus differences were found for retinol, retinyl palmitate, retinyl stearate, gamma-tocopherol and beta-carotene. Circulating nutrient concentrations for many of the species in this study have not been reported previously and most have not been compared with the animals' dietary intakes. The large number of animals analyzed provides a substantial base for comparing the serum nutrient concentrations of healthy animals, for both wild and captive exotic species.

  1. First records of parasitic copepods (Crustacea, Siphonostomatoida) from marine fishes in Korea.

    PubMed

    Venmathi Maran, B A; Soh, H Y; Hwang, U W; Chang, C Y; Myoung, J G

    2015-06-01

    The knowledge of the biodiversity of parasitic copepods in South Korea is increasing. Interestingly we report here, some parasitic copepods considered as the first record of findings from Korea. Nine species of parasitic copepods (Siphonostomatoida) including six genera of three different families [Caligidae (7), Lernaeopodidae (1), Lernanthropidae (1)] were recovered from eight species of wild fishes in Korea: 1) Caligus hoplognathi Yamaguti & Yamasu, 1959 (♀, ♂) from the body surface of barred knifejaw Oplegnathus fasciatus (Temminck & Schlegel); 2) Caligus lagocephali Pillai, 1961 (♀) from the gills of panther puffer Takifugu pardalis (Temminck & Schlegel); 3) Euryphorus brachypterus (Gerstaecker, 1853) (♀, ♂) from the opercular cavity of Atlantic bluefin tuna Thunnus thynnus (Linnaeus); 4) Euryphorus nordmanni Milne Edwards, 1840 (♀, ♂) from the opercular cavity of common dolphin fish Coryphaena hippurus Linnaeus; 5) Gloiopotes huttoni (Thomson) (♀, ♂) from the body surface of black marlin Istiompax indica (Cuvier); 6) Lepeophtheirus hapalogenyos Yamaguti & Yamasu, 1959 (♀) from the gill filaments of O. fasciatus; 7) Lepeophtheirus sekii Yamaguti, 1936 (♀, ♂) from the body surface of red seabream Pagrus major (Temminck & Schlegel); 8) Brachiella thynni Cuvier, 1830 (♀) from the body surface of longfin tuna or albacore Thunnus alalunga (Bonnaterre); 9) Lernanthropinus sphyraenae (Yamaguti & Yamasu, 1959) (♀) from the gill filaments of moon fish Mene maculata (Bloch & Schneider). Since the female was already reported in Korea, it is a new record for the male of C. hoplognathi. A checklist for the parasitic copepods of the family Caligidae, Lernaeopodidae and Lernanthropidae of Korea is provided.

  2. 21 CFR 822.14 - May I reference information previously submitted instead of submitting it again?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false May I reference information previously submitted..., DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES POSTMARKET SURVEILLANCE Postmarket Surveillance Plan § 822.14 May I reference information previously submitted instead of submitting it again? Yes...

  3. 31 CFR 356.14 - What are the requirements for submitting bids for customers?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... submitting bids for customers? 356.14 Section 356.14 Money and Finance: Treasury Regulations Relating to... requirements for submitting bids for customers? (a) Institutions that may submit bids for customers. Only depository institutions or dealers may submit bids for customers (see definitions at § 356.2), or for...

  4. Preventing High Blood Pressure

    MedlinePlus

    ... commit" type="submit" value="Submit" /> Related CDC Web Sites Division for Heart Disease and Stroke Prevention ... commit" type="submit" value="Submit" /> Related CDC Web Sites Division for Heart Disease and Stroke Prevention ...

  5. Cholesterol Facts and Statistics

    MedlinePlus

    ... commit" type="submit" value="Submit" /> Related CDC Web Sites Division for Heart Disease and Stroke Prevention ... commit" type="submit" value="Submit" /> Related CDC Web Sites Division for Heart Disease and Stroke Prevention ...

  6. Heart Disease Risk Factors

    MedlinePlus

    ... commit" type="submit" value="Submit" /> Related CDC Web Sites Division for Heart Disease and Stroke Prevention ... commit" type="submit" value="Submit" /> Related CDC Web Sites Division for Heart Disease and Stroke Prevention ...

  7. Men and Heart Disease

    MedlinePlus

    ... commit" type="submit" value="Submit" /> Related CDC Web Sites Heart Disease Stroke High Blood Pressure Salt ... commit" type="submit" value="Submit" /> Related CDC Web Sites Heart Disease Stroke High Blood Pressure Salt ...

  8. High Blood Pressure Facts

    MedlinePlus

    ... commit" type="submit" value="Submit" /> Related CDC Web Sites Division for Heart Disease and Stroke Prevention ... commit" type="submit" value="Submit" /> Related CDC Web Sites Division for Heart Disease and Stroke Prevention ...

  9. Prevention: What You Can Do

    MedlinePlus

    ... commit" type="submit" value="Submit" /> Related CDC Web Sites Division for Heart Disease and Stroke Prevention ... commit" type="submit" value="Submit" /> Related CDC Web Sites Division for Heart Disease and Stroke Prevention ...

  10. Data and Statistics: Women and Heart Disease

    MedlinePlus

    ... commit" type="submit" value="Submit" /> Related CDC Web Sites Heart Disease Stroke High Blood Pressure Salt ... commit" type="submit" value="Submit" /> Related CDC Web Sites Heart Disease Stroke High Blood Pressure Salt ...

  11. 30 CFR 1210.201 - How do I submit Form MMS-4430, Solid Minerals Production and Royalty Report?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...) Instructions for completing and submitting Form MMS-4430 are available on our Internet reporting web site or you may contact us toll free at 1-888-201-6416. (b) When to submit. (1) Unless your lease terms... month. (c) How to submit. (1) You must submit Form MMS-4430 electronically using our Internet reporting...

  12. American Indian and Alaska Native Heart Disease and Stroke

    MedlinePlus

    ... commit" type="submit" value="Submit" /> Related CDC Web Sites Heart Disease Stroke High Blood Pressure Salt ... commit" type="submit" value="Submit" /> Related CDC Web Sites Heart Disease Stroke High Blood Pressure Salt ...

  13. 32 CFR Appendix C to Part 282 - Submitting a Claim

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 32 National Defense 2 2010-07-01 2010-07-01 false Submitting a Claim C Appendix C to Part 282.... 282, App. C Appendix C to Part 282—Submitting a Claim (a) Who May Submit a Claim. Any person (“claimant”) may submit a claim who has a demand for money or property against the Government under 31 U.S.C...

  14. Values, animal symbolism, and human-animal relationships associated to two threatened felids in Mapuche and Chilean local narratives

    PubMed Central

    2013-01-01

    Background The Chilean temperate rainforest has been subjected to dramatic fragmentation for agriculture and forestry exploitation. Carnivore species are particularly affected by fragmentation and the resulting resource use conflicts with humans. This study aimed at understanding values and human-animal relationships with negatively perceived threatened carnivores through the disclosure of local stories and Mapuche traditional folktales. Methods Our mixed approach comprised the qualitative analysis of 112 stories on the kodkod cat (Leopardus guigna) and the puma (Puma concolor) collected by students (9-14 years) from 28 schools in the Araucania region within their family contexts, 10 qualitative in-depth interviews with indigenous Mapuche people, 35 traditional Mapuche legends, and the significance of naming found in ethnographic collections. Results We revealed a quasi-extinction of traditional tales in the current knowledge pool about pumas and kodkods, local anecdotes, however, were present in significant numbers. Values associated to both felids were manifold, ranging from negativistic to positive values. While pumas played an important role in people’s spirituality, negative mythological connotations persisted in kodkod stories. Four prominent relationships were derived: (1) Both felids represent threats to livestock, pumas even to life, (2) both felids are symbols for upcoming negative events, (3) pumas are spiritual creatures, and (4) kodkods are threatened by humans. Recommendations are provided for stimulating new ways of perceiving unpopular and threatened carnivores among those who live in vicinity to them. PMID:23764186

  15. Exposure to selected Pathogens in to selected pathogens in Geoffroy's cats and domestic carnivores from central Argentina.

    PubMed

    Uhart, Marcela M; Rago, M Virginia; Marull, Carolina A; Ferreyra, Hebe del Valle; Pereira, Javier A

    2012-10-01

    Wild carnivores share a high percentage of parasites and viruses with closely related domestic carnivores. Because of increased overlap and potential contact with domestic species, we conducted a retrospective serosurvey for 11 common carnivore pathogens in 40 Geoffroy's cats (Leopardus geoffroyi) sampled between 2000 and 2008 within or near two protected areas in central Argentina (Lihué Calel National Park, La Pampa, and Campos del Tuyú National Park, Buenos Aires), as well as five domestic cats and 11 domestic dogs from catde ranches adjacent to Lihué Calel Park. Geoffroy's cats had detectable antibody to canine distemper virus (CDV), feline calicivirus (FCV), feline coronavirus, feline panleukopenia virus (FPV), Toxoplasma gondii, Leptospira interrogans (serovars Ictero/Icter and Ballum), and Dirofilaria immitis. None of the wild cats had antibodies to feline herpesvirus, feline immunodeficiency virus (FIV), feline leukemia virus, or rabies virus. Domestic dogs had antibodies to CDV, canine adenovirus, canine herpesvirus, and canine parvovirus. Antibodies to FPV, FCV, FIV, and T. gondii were found in domestic cats. We provide the first data on exposure of free-ranging Geoffroy's cats to pathogens at two sites within the core area of the species distribution range, including the first report of antibodies to CDV in this species. We encourage continued monitoring for diseases in wild and domestic carnivores as well as preventive health care for domestic animals, particularly in park buffer zones where overlap is greatest.

  16. Two different groups of signal sequence in M-superfamily conotoxins.

    PubMed

    Wang, Qi; Jiang, Hui; Han, Yu-Hong; Yuan, Duo-Duo; Chi, Cheng-Wu

    2008-04-01

    M-superfamily conotoxins can be divided into four branches (M-1, M-2, M-3 and M-4) according to the number of amino acid residues in the third Cys loop. In general, it is widely accepted that the conotoxin signal peptides of each superfamily are strictly conserved. Recently, we cloned six cDNAs of novel M-superfamily conotoxins from Conus leopardus, Conus marmoreus and Conus quercinus, belonging to either M-1 or M-3 branch. These conotoxins, judging from the putative peptide sequences deducted from cDNAs, are rich in acidic residues and share highly conserved signal and pro-peptide region. However, they are quite different from the reported conotoxins of M-2 and M-4 branches even in their signal peptides, which in general are considered highly conserved for each superfamily of conotoxins. The signal sequences of M-1 and M-3 conotoxins composed of 24 residues start with MLKMGVVL-, while those of M-2 and M-4 conotoxins composed of 25 residues start with MMSKLGVL-. It is another example that different types of signal peptides can exist within a superfamily besides the I-conotoxin superfamily. In addition to the different disulfide connectivity of M-1 conotoxins from that of M-4 or M-2 conotoxins, the sequence alignment, preferential Cys codon usage and phylogenetic tree analysis suggest that M-1 and M-3 conotoxins have much closer relationship, being different from the conotoxins of other two branches (M-4 and M-2) of M-superfamily.

  17. 40 CFR 52.800 - Original identification of plan section.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... were submitted on the dates specified. (1) The State Air Pollution Control Board submitted a SO2 control strategy for the City of Indianapolis on March 16, 1972. (2) The Governor submitted Pub. L. 100...) The State Air Pollution Control Board submitted additional information on surveillance methodology...

  18. 40 CFR 52.800 - Original identification of plan section.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... were submitted on the dates specified. (1) The State Air Pollution Control Board submitted a SO2 control strategy for the City of Indianapolis on March 16, 1972. (2) The Governor submitted Pub. L. 100...) The State Air Pollution Control Board submitted additional information on surveillance methodology...

  19. 40 CFR 52.800 - Original identification of plan section.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... were submitted on the dates specified. (1) The State Air Pollution Control Board submitted a SO2 control strategy for the City of Indianapolis on March 16, 1972. (2) The Governor submitted Pub. L. 100...) The State Air Pollution Control Board submitted additional information on surveillance methodology...

  20. 40 CFR 60.2900 - When must I submit my waste management plan?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false When must I submit my waste management... Waste Management Plan § 60.2900 When must I submit my waste management plan? You must submit a waste management plan prior to commencing construction, reconstruction, or modification. ...

  1. 47 CFR 1.10017 - How can I submit additional information?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false How can I submit additional information? 1... International Bureau Filing System § 1.10017 How can I submit additional information? In response to an official request for information from the International Bureau, you can submit additional information...

  2. 40 CFR 35.105 - Time frame for submitting an application.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Time frame for submitting an application. 35.105 Section 35.105 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER....105 Time frame for submitting an application. An applicant should submit a complete application to EPA...

  3. 40 CFR 35.506 - Time frame for submitting an application.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 1 2010-07-01 2010-07-01 false Time frame for submitting an application. 35.506 Section 35.506 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY GRANTS AND OTHER... Application § 35.506 Time frame for submitting an application. An applicant should submit a complete...

  4. 40 CFR 60.2900 - When must I submit my waste management plan?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false When must I submit my waste management... Waste Management Plan § 60.2900 When must I submit my waste management plan? You must submit a waste management plan prior to commencing construction, reconstruction, or modification. ...

  5. 40 CFR 60.2900 - When must I submit my waste management plan?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 6 2011-07-01 2011-07-01 false When must I submit my waste management... Waste Management Plan § 60.2900 When must I submit my waste management plan? You must submit a waste management plan prior to commencing construction, reconstruction, or modification. ...

  6. 40 CFR 152.414 - Procedures.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... requesting a waiver. (1) A request for a waiver must be submitted in writing at the time the application is submitted to the Office of Pesticide Programs' Document Processing Desk at the appropriate address as set... document must be submitted with the waiver request that is submitted to the Office of Pesticide Programs...

  7. 76 FR 13300 - Procedures for Submitting to the Department of Energy Trade Secrets and Commercial or Financial...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-11

    ... procedures DOE uses to process loan applications submitted to DOE's Advanced Technology Vehicles... procedures DOE uses to process loan applications submitted to DOE's Advanced Technology Vehicles... requirements as described above for any information submitted through the Title XVII loan application process...

  8. 40 CFR 52.800 - Original identification of plan section.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... were submitted on the dates specified. (1) The State Air Pollution Control Board submitted a SO2 control strategy for the City of Indianapolis on March 16, 1972. (2) The Governor submitted Pub. L. 100...) The State Air Pollution Control Board submitted additional information on surveillance methodology...

  9. 41 CFR 102-75.855 - What happens if DOT does not submit an assignment recommendation?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... not submit an assignment recommendation? 102-75.855 Section 102-75.855 Public Contracts and Property Management Federal Property Management Regulations System (Continued) FEDERAL MANAGEMENT REGULATION REAL....855 What happens if DOT does not submit an assignment recommendation? If DOT does not submit an...

  10. 40 CFR 799.2155 - Commercial hexane.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... percent MCP. (b) Persons required to submit study plans, conduct tests, and submit data. All persons who... the end of the reimbursement period shall submit letters of intent to conduct testing, submit study... study plans for those tests less than 45 days before beginning testing provided that EPA receives the...

  11. The Treatment of BRCA1/2 Hereditary Breast Cancer and Sporadic Breast Cancer with Poly(ADP-ribose) PARP-1 Inhibitors and Chemotherapy

    DTIC Science & Technology

    2008-09-01

    American population d) D) Obesity, and breast cancer J. of Nursing and Bariatric Surgery . 2008 submitting. This paper uses in part mechanisms worked...National Med. Society. 2008 submitting D) Obesity, and breast cancer J. of Nursing and Bariatric Surgery . 2008 submitting Abstracts: A) De Soto JA...submitting De Soto JA. Obesity, breast cancer and bariatric surgery . J. of Nursing and Bariatric Surgery . 2008 submitting Davis JH, De Soto JA

  12. 39 CFR 3007.10 - Submission of non-public materials under seal.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...-public materials shall submit two copies consisting, where practicable, of two paper hard copies as well... available PC application. Each page of any paper hard copy non-public materials submitted shall be clearly... submitted in a searchable electronic format, but need not be submitted in its native format. As part of its...

  13. 25 CFR 559.7 - May a tribe submit documents required by this part electronically?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false May a tribe submit documents required by this part... NOTIFICATIONS AND SUBMISSIONS § 559.7 May a tribe submit documents required by this part electronically? Yes. Tribes wishing to submit documents electronically should contact the Commission for guidance on...

  14. 25 CFR 559.7 - May a tribe submit documents required by this part electronically?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false May a tribe submit documents required by this part... NOTIFICATIONS AND SUBMISSIONS § 559.7 May a tribe submit documents required by this part electronically? Yes. Tribes wishing to submit documents electronically should contact the Commission for guidance on...

  15. 76 FR 75894 - Information Collection Activities: Pipelines and Pipeline Rights-of-Way; Submitted for Office of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-05

    ... pipelines `` * * * for the transportation of oil, natural gas, sulphur, or other minerals, or under such...) Submit repair report 3 1008(f) Submit report of pipeline failure analysis...... 30 1008(g) Submit plan of.... BSEE-2011-0002; OMB Control Number 1010-0050] Information Collection Activities: Pipelines and Pipeline...

  16. 40 CFR 62.14715 - When must I submit my waste management plan?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 8 2010-07-01 2010-07-01 false When must I submit my waste management... submit my waste management plan? You must submit the waste management plan no later than April 5, 2004. ... POLLUTANTS Federal Plan Requirements for Commercial and Industrial Solid Waste Incineration Units That...

  17. 21 CFR 20.114 - Data and information submitted pursuant to cooperative quality assurance agreements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... Records § 20.114 Data and information submitted pursuant to cooperative quality assurance agreements. Data and information submitted to the Food and Drug Administration pursuant to a cooperative quality... 21 Food and Drugs 1 2014-04-01 2014-04-01 false Data and information submitted pursuant to...

  18. 21 CFR 20.114 - Data and information submitted pursuant to cooperative quality assurance agreements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Records § 20.114 Data and information submitted pursuant to cooperative quality assurance agreements. Data and information submitted to the Food and Drug Administration pursuant to a cooperative quality... 21 Food and Drugs 1 2011-04-01 2011-04-01 false Data and information submitted pursuant to...

  19. 21 CFR 20.114 - Data and information submitted pursuant to cooperative quality assurance agreements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Records § 20.114 Data and information submitted pursuant to cooperative quality assurance agreements. Data and information submitted to the Food and Drug Administration pursuant to a cooperative quality... 21 Food and Drugs 1 2012-04-01 2012-04-01 false Data and information submitted pursuant to...

  20. 21 CFR 20.114 - Data and information submitted pursuant to cooperative quality assurance agreements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Records § 20.114 Data and information submitted pursuant to cooperative quality assurance agreements. Data and information submitted to the Food and Drug Administration pursuant to a cooperative quality... 21 Food and Drugs 1 2013-04-01 2013-04-01 false Data and information submitted pursuant to...

  1. 77 FR 54904 - Notice Inviting Publishers To Submit Tests for a Determination of Suitability for Use in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-06

    ... DEPARTMENT OF EDUCATION Notice Inviting Publishers To Submit Tests for a Determination of... applications: October 1, 2012. SUMMARY: The Secretary of Education (1) invites publishers to submit tests for... the date by which publishers must submit these tests. FOR FURTHER INFORMATION CONTACT: John LeMaster...

  2. 78 FR 48152 - Notice Inviting Publishers To Submit Tests for a Determination of Suitability for Use in the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-07

    ... DEPARTMENT OF EDUCATION Notice Inviting Publishers To Submit Tests for a Determination of... applications: October 1, 2013. SUMMARY: The Secretary of Education (1) invites publishers to submit tests for... the date by which publishers must submit these tests. FOR FURTHER INFORMATION CONTACT: Michelle Meier...

  3. 40 CFR 62.14715 - When must I submit my waste management plan?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 9 2013-07-01 2013-07-01 false When must I submit my waste management... submit my waste management plan? You must submit the waste management plan no later than April 5, 2004. ... POLLUTANTS Federal Plan Requirements for Commercial and Industrial Solid Waste Incineration Units That...

  4. 40 CFR 62.14715 - When must I submit my waste management plan?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 9 2014-07-01 2014-07-01 false When must I submit my waste management... submit my waste management plan? You must submit the waste management plan no later than April 5, 2004. ... POLLUTANTS Federal Plan Requirements for Commercial and Industrial Solid Waste Incineration Units That...

  5. 40 CFR 62.14715 - When must I submit my waste management plan?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 9 2012-07-01 2012-07-01 false When must I submit my waste management... submit my waste management plan? You must submit the waste management plan no later than April 5, 2004. ... POLLUTANTS Federal Plan Requirements for Commercial and Industrial Solid Waste Incineration Units That...

  6. 27 CFR 73.31 - May I submit forms electronically to TTB?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...; ELECTRONIC SUBMISSION OF FORMS Electronic Filing of Documents with TTB § 73.31 May I submit forms... above; (c) You submit the electronic form to an electronic document receiving system that we have... submit the form through an electronic document receiving system that TTB has designated for the receipt...

  7. 30 CFR 210.203 - How do I submit sales contracts?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... royalty terms. (b) When to submit. (1) For coal and metal production, you must submit the required... MANAGEMENT FORMS AND REPORTS Production and Royalty Reports-Solid Minerals § 210.203 How do I submit sales..., and phosphate production, and production from any other lease with ad valorem royalty terms, you must...

  8. 40 CFR 62.14715 - When must I submit my waste management plan?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... POLLUTANTS Federal Plan Requirements for Commercial and Industrial Solid Waste Incineration Units That... 40 Protection of Environment 8 2011-07-01 2011-07-01 false When must I submit my waste management... submit my waste management plan? You must submit the waste management plan no later than April 5, 2004. ...

  9. 30 CFR 254.53 - Submitting a response plan developed under State requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Submitting a response plan developed under....53 Submitting a response plan developed under State requirements. (a) You may submit a response plan... Federal officials and response personnel if there is a spill. (3) Identify any private personnel and...

  10. 78 FR 54637 - Agency Information Collection Activities; Comment Request; State Educational Agency, Local...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-05

    ... requests have occurred in several iterations: Window 1, for which SEAs submitted requests in November 2011; Window 2, for which SEAs submitted requests in February 2012; Window 3, for which SEAs submitted requests in September 2012; and Window 4, for which SEAs submitted requests in spring 2013. Generally, ED...

  11. 12 CFR 516.110 - Who may submit a written comment?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 12 Banks and Banking 5 2010-01-01 2010-01-01 false Who may submit a written comment? 516.110 Section 516.110 Banks and Banking OFFICE OF THRIFT SUPERVISION, DEPARTMENT OF THE TREASURY APPLICATION PROCESSING PROCEDURES Comment Procedures § 516.110 Who may submit a written comment? Any person may submit a...

  12. Audit of the practice of sputum smear examination for patients with suspected pulmonary tuberculosis in Fiji.

    PubMed

    Gounder, Shakti; Tayler-Smith, Katherine; Khogali, Mohammed; Raikabula, Maopa; Harries, Anthony D

    2013-07-01

    In Fiji, patients with suspected pulmonary tuberculosis (PTB) currently submit three sputum specimens for smear microscopy for acid-fast bacilli, but there is little information about how well this practice is carried out. A cross-sectional retrospective review was carried out in all four TB diagnostic laboratories in Fiji to determine among new patients presenting with suspected PTB in 2011: the quality of submitted sputum; the number of sputum samples submitted; the relationship between quality and number of submitted samples to smear-positivity; and positive yield from first, second and third samples. Of 1940 patients with suspected PTB, 3522 sputum samples were submitted: 997 (51.4%) patients submitted one sample, 304 (15.7%) patients submitted two samples and 639 (32.9%) submitted three samples. Sputum quality was recorded in 2528 (71.8%) of samples, of which 1046 (41.4%) were of poor quality. Poor quality sputum was more frequent in females, inpatients and children (0-14 years). Good quality sputum and a higher number of submitted samples positively correlated with smear-positivity for acid-fast bacilli. There were 122 (6.3%) patients with suspected PTB who were sputum smear positive. Of those, 89 had submitted three sputum samples: 79 (89%) were diagnosed based on the first sputum sample, 6 (7%) on the second sample and 4 (4%) on the third sample. This study shows that there are deficiencies in the practice of sputum smear examination in Fiji with respect to sputum quality and recommended number of submitted samples, although the results support the continued use of three sputum samples for TB diagnosis. Ways to improve sputum quality and adherence to recommended guidelines are needed.

  13. 20 CFR 220.48 - If the claimant fails to submit medical or other evidence.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 1 2013-04-01 2012-04-01 true If the claimant fails to submit medical or other evidence. 220.48 Section 220.48 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER... fails to submit medical or other evidence. The Board may request a claimant to submit medical or other...

  14. 20 CFR 220.48 - If the claimant fails to submit medical or other evidence.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false If the claimant fails to submit medical or other evidence. 220.48 Section 220.48 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER... fails to submit medical or other evidence. The Board may request a claimant to submit medical or other...

  15. 20 CFR 220.48 - If the claimant fails to submit medical or other evidence.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 1 2011-04-01 2011-04-01 false If the claimant fails to submit medical or other evidence. 220.48 Section 220.48 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER... fails to submit medical or other evidence. The Board may request a claimant to submit medical or other...

  16. 20 CFR 220.48 - If the claimant fails to submit medical or other evidence.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 1 2014-04-01 2012-04-01 true If the claimant fails to submit medical or other evidence. 220.48 Section 220.48 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER... fails to submit medical or other evidence. The Board may request a claimant to submit medical or other...

  17. 20 CFR 220.48 - If the claimant fails to submit medical or other evidence.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 1 2012-04-01 2012-04-01 false If the claimant fails to submit medical or other evidence. 220.48 Section 220.48 Employees' Benefits RAILROAD RETIREMENT BOARD REGULATIONS UNDER... fails to submit medical or other evidence. The Board may request a claimant to submit medical or other...

  18. 45 CFR 149.320 - Universe of claims that must be submitted.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 45 Public Welfare 1 2012-10-01 2012-10-01 false Universe of claims that must be submitted. 149.320 Section 149.320 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH... Universe of claims that must be submitted. (a) Claims submitted for an early retiree, as defined in § 149.2...

  19. 45 CFR 149.320 - Universe of claims that must be submitted.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 45 Public Welfare 1 2014-10-01 2014-10-01 false Universe of claims that must be submitted. 149.320 Section 149.320 Public Welfare Department of Health and Human Services REQUIREMENTS RELATING TO HEALTH... Universe of claims that must be submitted. (a) Claims submitted for an early retiree, as defined in § 149.2...

  20. 45 CFR 149.320 - Universe of claims that must be submitted.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 45 Public Welfare 1 2013-10-01 2013-10-01 false Universe of claims that must be submitted. 149.320 Section 149.320 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH... Universe of claims that must be submitted. (a) Claims submitted for an early retiree, as defined in § 149.2...

  1. 45 CFR 149.320 - Universe of claims that must be submitted.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 1 2011-10-01 2011-10-01 false Universe of claims that must be submitted. 149.320 Section 149.320 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH... Universe of claims that must be submitted. (a) Claims submitted for an early retiree, as defined in § 149.2...

  2. 30 CFR 250.287 - For what development projects must I submit a DWOP?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false For what development projects must I submit a... Operations Plans (dwop) § 250.287 For what development projects must I submit a DWOP? You must submit a DWOP for each development project in which you will use non-conventional production or completion...

  3. 76 FR 57944 - Fisheries of the Northeastern United States; Northeast Multispecies Fisheries, Small-Mesh...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-19

    ..., identified by NOAA-NMFS-2011-0206, by any of the following methods: Electronic Submission: Submit all... click on the ``Submit a Comment'' icon on the right of that line. Mail: Submit written comments to...-9135; Attn: Moira Kelly. Instructions: Comments must be submitted by one of the above methods to ensure...

  4. 77 FR 31562 - Atlantic Highly Migratory Species; Atlantic Shark Management Measures

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-29

    ... document, identified by NOAA-NMFS-2011-0229, by any of the following methods: Electronic Submission: Submit... click on the ``Submit a Comment'' icon on the right of that line. Mail: Submit written comments to Peter...: (301) 713-1917. Attn: Peter Cooper. Comments must be submitted by one of the above methods to ensure...

  5. 47 CFR 36.612 - Updating information submitted to the National Exchange Carrier Association.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... update the information submitted to the National Exchange Carrier Association (NECA) on July 31st... non-rural telephone company must update the information submitted to NECA on July 31st pursuant to § 36.611 (h) according to the schedule. (1) Submit data covering the last nine months of the previous...

  6. 47 CFR 54.1306 - Updating Information Submitted to the National Exchange Carrier Association.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... incumbent local exchange carrier subject to § 54.1301(a) may update the information submitted to the... on a rolling year basis according to the schedule. (1) Submit data covering the last nine months of... September 30th of the existing year; (2) Submit data covering the last six months of the previous calendar...

  7. 47 CFR 36.612 - Updating information submitted to the National Exchange Carrier Association.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., may update the information submitted to the National Exchange Carrier Association (NECA) on July 31st... must update the information submitted to NECA on July 31st pursuant to § 36.611(h) according to the schedule. Every non-rural telephone company must update the information submitted to NECA on July 31st...

  8. 47 CFR 36.612 - Updating information submitted to the National Exchange Carrier Association.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ..., may update the information submitted to the National Exchange Carrier Association (NECA) on July 31st... must update the information submitted to NECA on July 31st pursuant to § 36.611(h) according to the schedule. Every non-rural telephone company must update the information submitted to NECA on July 31st...

  9. 47 CFR 36.612 - Updating information submitted to the National Exchange Carrier Association.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... update the information submitted to the National Exchange Carrier Association (NECA) on July 31st... non-rural telephone company must update the information submitted to NECA on July 31st pursuant to § 36.611 (h) according to the schedule. (1) Submit data covering the last nine months of the previous...

  10. 25 CFR 224.54 - How must a tribe submit an application?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 1 2014-04-01 2014-04-01 false How must a tribe submit an application? 224.54 Section... Obtaining Tribal Energy Resource Agreements Processing Applications § 224.54 How must a tribe submit an application? A tribe must submit an application and all supporting documents in written and electronic form to...

  11. 25 CFR 183.15 - Must the Tribe submit any reports?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Must the Tribe submit any reports? 183.15 Section 183.15... SAN CARLOS APACHE TRIBE DEVELOPMENT TRUST FUND AND SAN CARLOS APACHE TRIBE LEASE FUND Reports § 183.15 Must the Tribe submit any reports? Yes. The Tribe must submit the following reports after receiving...

  12. 25 CFR 224.54 - How must a tribe submit an application?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 1 2012-04-01 2011-04-01 true How must a tribe submit an application? 224.54 Section 224... Obtaining Tribal Energy Resource Agreements Processing Applications § 224.54 How must a tribe submit an application? A tribe must submit an application and all supporting documents in written and electronic form to...

  13. 25 CFR 224.54 - How must a tribe submit an application?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 1 2013-04-01 2013-04-01 false How must a tribe submit an application? 224.54 Section... Obtaining Tribal Energy Resource Agreements Processing Applications § 224.54 How must a tribe submit an application? A tribe must submit an application and all supporting documents in written and electronic form to...

  14. 25 CFR 183.15 - Must the Tribe submit any reports?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 1 2013-04-01 2013-04-01 false Must the Tribe submit any reports? 183.15 Section 183.15... SAN CARLOS APACHE TRIBE DEVELOPMENT TRUST FUND AND SAN CARLOS APACHE TRIBE LEASE FUND Reports § 183.15 Must the Tribe submit any reports? Yes. The Tribe must submit the following reports after receiving...

  15. 25 CFR 183.15 - Must the Tribe submit any reports?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 25 Indians 1 2012-04-01 2011-04-01 true Must the Tribe submit any reports? 183.15 Section 183.15... SAN CARLOS APACHE TRIBE DEVELOPMENT TRUST FUND AND SAN CARLOS APACHE TRIBE LEASE FUND Reports § 183.15 Must the Tribe submit any reports? Yes. The Tribe must submit the following reports after receiving...

  16. 25 CFR 183.15 - Must the Tribe submit any reports?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 1 2014-04-01 2014-04-01 false Must the Tribe submit any reports? 183.15 Section 183.15... SAN CARLOS APACHE TRIBE DEVELOPMENT TRUST FUND AND SAN CARLOS APACHE TRIBE LEASE FUND Reports § 183.15 Must the Tribe submit any reports? Yes. The Tribe must submit the following reports after receiving...

  17. 25 CFR 183.15 - Must the Tribe submit any reports?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false Must the Tribe submit any reports? 183.15 Section 183.15... SAN CARLOS APACHE TRIBE DEVELOPMENT TRUST FUND AND SAN CARLOS APACHE TRIBE LEASE FUND Reports § 183.15 Must the Tribe submit any reports? Yes. The Tribe must submit the following reports after receiving...

  18. 25 CFR 224.54 - How must a tribe submit an application?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 25 Indians 1 2011-04-01 2011-04-01 false How must a tribe submit an application? 224.54 Section... Obtaining Tribal Energy Resource Agreements Processing Applications § 224.54 How must a tribe submit an application? A tribe must submit an application and all supporting documents in written and electronic form to...

  19. 30 CFR 210.154 - What documents or other information must I submit for Federal oil valuation purposes?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and Reports-Oil, Gas, and Geothermal Resources § 210.154 What documents or other information must I submit for Federal oil valuation purposes? (a) General. The MMS may require you to submit documents or... submit for Federal oil valuation purposes? 210.154 Section 210.154 Mineral Resources MINERALS MANAGEMENT...

  20. 34 CFR 75.126 - Application must list all programs to which it is submitted.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false Application must list all programs to which it is submitted. 75.126 Section 75.126 Education Office of the Secretary, Department of Education DIRECT GRANT... all programs to which it is submitted. If an applicant is submitting an application for the same...

  1. 30 CFR 250.287 - For what development projects must I submit a DWOP?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... and Information Deepwater Operations Plan (dwop) § 250.287 For what development projects must I submit a DWOP? You must submit a DWOP for each development project in which you will use non-conventional... 30 Mineral Resources 2 2014-07-01 2014-07-01 false For what development projects must I submit a...

  2. 30 CFR 250.287 - For what development projects must I submit a DWOP?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... and Information Deepwater Operations Plan (dwop) § 250.287 For what development projects must I submit a DWOP? You must submit a DWOP for each development project in which you will use non-conventional... 30 Mineral Resources 2 2013-07-01 2013-07-01 false For what development projects must I submit a...

  3. 30 CFR 250.287 - For what development projects must I submit a DWOP?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... and Information Deepwater Operations Plan (dwop) § 250.287 For what development projects must I submit a DWOP? You must submit a DWOP for each development project in which you will use non-conventional... 30 Mineral Resources 2 2012-07-01 2012-07-01 false For what development projects must I submit a...

  4. 30 CFR 250.287 - For what development projects must I submit a DWOP?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... SHELF Plans and Information Deepwater Operations Plans (dwop) § 250.287 For what development projects must I submit a DWOP? You must submit a DWOP for each development project in which you will use non... 30 Mineral Resources 2 2011-07-01 2011-07-01 false For what development projects must I submit a...

  5. 17 CFR 401.2 - Exemption for depository institutions that submit tenders for the account of customers for...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... institutions that submit tenders for the account of customers for purchase on original issue of United States... depository institutions that submit tenders for the account of customers for purchase on original issue of... depository institution that submits tenders or subscriptions for purchase on original issue of United States...

  6. 17 CFR 401.2 - Exemption for depository institutions that submit tenders for the account of customers for...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... institutions that submit tenders for the account of customers for purchase on original issue of United States... depository institutions that submit tenders for the account of customers for purchase on original issue of... depository institution that submits tenders or subscriptions for purchase on original issue of United States...

  7. 17 CFR 401.2 - Exemption for depository institutions that submit tenders for the account of customers for...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... institutions that submit tenders for the account of customers for purchase on original issue of United States... depository institutions that submit tenders for the account of customers for purchase on original issue of... depository institution that submits tenders or subscriptions for purchase on original issue of United States...

  8. 17 CFR 401.2 - Exemption for depository institutions that submit tenders for the account of customers for...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... institutions that submit tenders for the account of customers for purchase on original issue of United States... depository institutions that submit tenders for the account of customers for purchase on original issue of... depository institution that submits tenders or subscriptions for purchase on original issue of United States...

  9. 17 CFR 401.2 - Exemption for depository institutions that submit tenders for the account of customers for...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... institutions that submit tenders for the account of customers for purchase on original issue of United States... depository institutions that submit tenders for the account of customers for purchase on original issue of... depository institution that submits tenders or subscriptions for purchase on original issue of United States...

  10. 40 CFR 62.14585 - When must I submit my waste management plan?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 8 2010-07-01 2010-07-01 false When must I submit my waste management... Commenced Construction On or Before November 30, 1999 Waste Management Plan § 62.14585 When must I submit my waste management plan? You must submit a waste management plan no later than April 5, 2004. ...

  11. 40 CFR 60.2060 - When must I submit my waste management plan?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false When must I submit my waste management plan? 60.2060 Section 60.2060 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR... Management Plan § 60.2060 When must I submit my waste management plan? You must submit a waste management...

  12. 40 CFR 60.2060 - When must I submit my waste management plan?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false When must I submit my waste management plan? 60.2060 Section 60.2060 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR... Management Plan § 60.2060 When must I submit my waste management plan? You must submit a waste management...

  13. 40 CFR 60.2060 - When must I submit my waste management plan?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 6 2011-07-01 2011-07-01 false When must I submit my waste management plan? 60.2060 Section 60.2060 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR... Management Plan § 60.2060 When must I submit my waste management plan? You must submit a waste management...

  14. 78 FR 17 - Business Conduct and Documentation Requirements for Swap Dealers and Major Swap Participants...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-02

    ... submit your comments using only one method. All comments must be submitted in English, or if not, accompanied by an English translation. Comments will be posted as received to www.cftc.gov . You should submit...-based solution to automate the information-gathering process and provide sharing of submitted data and...

  15. 40 CFR 60.3011 - When must I submit my waste management plan?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 7 2014-07-01 2014-07-01 false When must I submit my waste management... Model Rule-Waste Management Plan § 60.3011 When must I submit my waste management plan? You must submit a waste management plan no later than 60 days following the initial performance test as specified in...

  16. 40 CFR 62.14585 - When must I submit my waste management plan?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 9 2014-07-01 2014-07-01 false When must I submit my waste management... Commenced Construction On or Before November 30, 1999 Waste Management Plan § 62.14585 When must I submit my waste management plan? You must submit a waste management plan no later than April 5, 2004. ...

  17. 40 CFR 60.3011 - When must I submit my waste management plan?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 6 2011-07-01 2011-07-01 false When must I submit my waste management... Model Rule-Waste Management Plan § 60.3011 When must I submit my waste management plan? You must submit a waste management plan no later than 60 days following the initial performance test as specified in...

  18. 40 CFR 60.2900 - When must I submit my waste management plan?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 7 2014-07-01 2014-07-01 false When must I submit my waste management... Modification or Reconstruction is Commenced on or After June 16, 2006 Waste Management Plan § 60.2900 When must I submit my waste management plan? You must submit a waste management plan prior to commencing...

  19. 40 CFR 60.2625 - When must I submit my waste management plan?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false When must I submit my waste management... Compliance Times for Commercial and Industrial Solid Waste Incineration Units Model Rule-Waste Management Plan § 60.2625 When must I submit my waste management plan? You must submit a waste management plan no...

  20. 40 CFR 60.2755 - When must I submit my waste management plan?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false When must I submit my waste management... Compliance Times for Commercial and Industrial Solid Waste Incineration Units Model Rule-Recordkeeping and Reporting § 60.2755 When must I submit my waste management plan? You must submit the waste management plan...

  1. 40 CFR 62.14585 - When must I submit my waste management plan?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 9 2013-07-01 2013-07-01 false When must I submit my waste management... Commenced Construction On or Before November 30, 1999 Waste Management Plan § 62.14585 When must I submit my waste management plan? You must submit a waste management plan no later than April 5, 2004. ...

  2. 40 CFR 60.3011 - When must I submit my waste management plan?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 7 2013-07-01 2013-07-01 false When must I submit my waste management... Model Rule-Waste Management Plan § 60.3011 When must I submit my waste management plan? You must submit a waste management plan no later than 60 days following the initial performance test as specified in...

  3. 40 CFR 60.2900 - When must I submit my waste management plan?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 7 2013-07-01 2013-07-01 false When must I submit my waste management... Modification or Reconstruction is Commenced on or After June 16, 2006 Waste Management Plan § 60.2900 When must I submit my waste management plan? You must submit a waste management plan prior to commencing...

  4. 40 CFR 62.14585 - When must I submit my waste management plan?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 9 2012-07-01 2012-07-01 false When must I submit my waste management... Commenced Construction On or Before November 30, 1999 Waste Management Plan § 62.14585 When must I submit my waste management plan? You must submit a waste management plan no later than April 5, 2004. ...

  5. 40 CFR 60.3011 - When must I submit my waste management plan?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 7 2012-07-01 2012-07-01 false When must I submit my waste management... Model Rule-Waste Management Plan § 60.3011 When must I submit my waste management plan? You must submit a waste management plan no later than 60 days following the initial performance test as specified in...

  6. 13 CFR 120.830 - Reports a CDC must submit.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Reports a CDC must submit. 120.830... Company Loan Program (504) Requirements for Cdc Certification and Operation § 120.830 Reports a CDC must submit. A CDC must submit the following reports to SBA: (a) An annual report within one hundred-eighty...

  7. 13 CFR 120.830 - Reports a CDC must submit.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 13 Business Credit and Assistance 1 2013-01-01 2013-01-01 false Reports a CDC must submit. 120.830... Company Loan Program (504) Requirements for Cdc Certification and Operation § 120.830 Reports a CDC must submit. A CDC must submit the following reports to SBA: (a) An annual report within one hundred-eighty...

  8. 13 CFR 120.830 - Reports a CDC must submit.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 13 Business Credit and Assistance 1 2011-01-01 2011-01-01 false Reports a CDC must submit. 120.830... Company Loan Program (504) Requirements for Cdc Certification and Operation § 120.830 Reports a CDC must submit. A CDC must submit the following reports to SBA: (a) An annual report within one hundred-eighty...

  9. 13 CFR 120.830 - Reports a CDC must submit.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 13 Business Credit and Assistance 1 2012-01-01 2012-01-01 false Reports a CDC must submit. 120.830... Company Loan Program (504) Requirements for Cdc Certification and Operation § 120.830 Reports a CDC must submit. A CDC must submit the following reports to SBA: (a) An annual report within one hundred-eighty...

  10. 30 CFR 254.53 - Submitting a response plan developed under State requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 2 2012-07-01 2012-07-01 false Submitting a response plan developed under... of the Coast Line § 254.53 Submitting a response plan developed under State requirements. (a) You may submit a response plan to BSEE for approval that you developed in accordance with the laws or regulations...

  11. 30 CFR 254.53 - Submitting a response plan developed under State requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false Submitting a response plan developed under... of the Coast Line § 254.53 Submitting a response plan developed under State requirements. (a) You may submit a response plan to BSEE for approval that you developed in accordance with the laws or regulations...

  12. 30 CFR 254.53 - Submitting a response plan developed under State requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 2 2011-07-01 2011-07-01 false Submitting a response plan developed under... Waters Seaward of the Coast Line § 254.53 Submitting a response plan developed under State requirements. (a) You may submit a response plan to MMS for approval that you developed in accordance with the laws...

  13. 30 CFR 254.53 - Submitting a response plan developed under State requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 2 2014-07-01 2014-07-01 false Submitting a response plan developed under... of the Coast Line § 254.53 Submitting a response plan developed under State requirements. (a) You may submit a response plan to BSEE for approval that you developed in accordance with the laws or regulations...

  14. 25 CFR 514.15 - May tribes submit fingerprint cards to the NIGC for processing?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 25 Indians 2 2013-04-01 2013-04-01 false May tribes submit fingerprint cards to the NIGC for... GENERAL PROVISIONS FEES § 514.15 May tribes submit fingerprint cards to the NIGC for processing? Tribes may submit fingerprint cards to the Commission for processing by the Federal Bureau of Investigation...

  15. 25 CFR 514.15 - May tribes submit fingerprint cards to the NIGC for processing?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 25 Indians 2 2014-04-01 2014-04-01 false May tribes submit fingerprint cards to the NIGC for... GENERAL PROVISIONS FEES § 514.15 May tribes submit fingerprint cards to the NIGC for processing? Tribes may submit fingerprint cards to the Commission for processing by the Federal Bureau of Investigation...

  16. 40 CFR 60.3011 - When must I submit my waste management plan?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false When must I submit my waste management... Times for Other Solid Waste Incineration Units That Commenced Construction On or Before December 9, 2004 Model Rule-Waste Management Plan § 60.3011 When must I submit my waste management plan? You must submit...

  17. 30 CFR 1218.580 - When do I submit Form ONRR-4444?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 3 2014-07-01 2014-07-01 false When do I submit Form ONRR-4444? 1218.580... Service of Official Correspondence § 1218.580 When do I submit Form ONRR-4444? Initially, you must submit Form ONRR-4444 by November 29, 2006, and subsequently, within 2 weeks of any change of your address. ...

  18. 30 CFR 1218.580 - When do I submit Form MMS-4444?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 3 2012-07-01 2012-07-01 false When do I submit Form MMS-4444? 1218.580... Service of Official Correspondence § 1218.580 When do I submit Form MMS-4444? Initially, you must submit Form MMS-4444 by November 29, 2006, and subsequently, within 2 weeks of any change of your address. ...

  19. 30 CFR 218.580 - When do I submit Form MMS-4444?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false When do I submit Form MMS-4444? 218.580 Section 218.580 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR MINERALS REVENUE... Correspondence § 218.580 When do I submit Form MMS-4444? Initially, you must submit MMS Form-4444 by November 29...

  20. 30 CFR 1218.580 - When do I submit Form MMS-4444?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 30 Mineral Resources 3 2011-07-01 2011-07-01 false When do I submit Form MMS-4444? 1218.580... Service of Official Correspondence § 1218.580 When do I submit Form MMS-4444? Initially, you must submit Form MMS-4444 by November 29, 2006, and subsequently, within 2 weeks of any change of your address. ...

  1. 30 CFR 1218.580 - When do I submit Form ONRR-4444?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 3 2013-07-01 2013-07-01 false When do I submit Form ONRR-4444? 1218.580... Service of Official Correspondence § 1218.580 When do I submit Form ONRR-4444? Initially, you must submit Form ONRR-4444 by November 29, 2006, and subsequently, within 2 weeks of any change of your address. ...

  2. 30 CFR 1210.201 - How do I submit Form ONRR-4430, Solid Minerals Production and Royalty Report?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... and submitting Form ONRR-4430 are available on our Internet reporting web site or you may contact us toll free at 1-888-201-6416. (b) When to submit. (1) Unless your lease terms specify a different... submit Form ONRR-4430 electronically using our Internet reporting web site unless you meet the conditions...

  3. 30 CFR 1210.201 - How do I submit Form ONRR-4430, Solid Minerals Production and Royalty Report?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... and submitting Form ONRR-4430 are available on our Internet reporting web site or you may contact us toll free at 1-888-201-6416. (b) When to submit. (1) Unless your lease terms specify a different... submit Form ONRR-4430 electronically using our Internet reporting web site unless you meet the conditions...

  4. 20 CFR 10.536 - What is the penalty for failing to submit a report of dependents?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 20 Employees' Benefits 1 2010-04-01 2010-04-01 false What is the penalty for failing to submit a...' COMPENSATION ACT, AS AMENDED Continuing Benefits Reports of Dependents § 10.536 What is the penalty for failing to submit a report of dependents? If an employee fails to submit a requested statement or supporting...

  5. 49 CFR 655.49 - Refusal to submit to a drug or alcohol test.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... IN TRANSIT OPERATIONS Types of Testing § 655.49 Refusal to submit to a drug or alcohol test. (a) Each employer shall require a covered employee to submit to a post-accident drug and alcohol test required under... 49 Transportation 7 2013-10-01 2013-10-01 false Refusal to submit to a drug or alcohol test. 655...

  6. 49 CFR 655.49 - Refusal to submit to a drug or alcohol test.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... IN TRANSIT OPERATIONS Types of Testing § 655.49 Refusal to submit to a drug or alcohol test. (a) Each employer shall require a covered employee to submit to a post-accident drug and alcohol test required under... 49 Transportation 7 2011-10-01 2011-10-01 false Refusal to submit to a drug or alcohol test. 655...

  7. 49 CFR 655.49 - Refusal to submit to a drug or alcohol test.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... IN TRANSIT OPERATIONS Types of Testing § 655.49 Refusal to submit to a drug or alcohol test. (a) Each employer shall require a covered employee to submit to a post-accident drug and alcohol test required under... 49 Transportation 7 2014-10-01 2014-10-01 false Refusal to submit to a drug or alcohol test. 655...

  8. 49 CFR 655.49 - Refusal to submit to a drug or alcohol test.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... IN TRANSIT OPERATIONS Types of Testing § 655.49 Refusal to submit to a drug or alcohol test. (a) Each employer shall require a covered employee to submit to a post-accident drug and alcohol test required under... 49 Transportation 7 2012-10-01 2012-10-01 false Refusal to submit to a drug or alcohol test. 655...

  9. 49 CFR 655.49 - Refusal to submit to a drug or alcohol test.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... IN TRANSIT OPERATIONS Types of Testing § 655.49 Refusal to submit to a drug or alcohol test. (a) Each employer shall require a covered employee to submit to a post-accident drug and alcohol test required under... 49 Transportation 7 2010-10-01 2010-10-01 false Refusal to submit to a drug or alcohol test. 655...

  10. 30 CFR 250.288 - When and how must I submit the Conceptual Plan?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... OFFSHORE OIL AND GAS AND SULPHUR OPERATIONS IN THE OUTER CONTINENTAL SHELF Plans and Information Deepwater Operations Plans (dwop) § 250.288 When and how must I submit the Conceptual Plan? You must submit four copies... 30 Mineral Resources 2 2010-07-01 2010-07-01 false When and how must I submit the Conceptual Plan...

  11. 42 CFR 137.132 - How does the Indian Tribe submit a final offer?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false How does the Indian Tribe submit a final offer? 137.132 Section 137.132 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES....132 How does the Indian Tribe submit a final offer? (a) A written final offer should be submitted: (1...

  12. 42 CFR 137.132 - How does the Indian Tribe submit a final offer?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false How does the Indian Tribe submit a final offer? 137.132 Section 137.132 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES....132 How does the Indian Tribe submit a final offer? (a) A written final offer should be submitted: (1...

  13. 42 CFR 137.132 - How does the Indian Tribe submit a final offer?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false How does the Indian Tribe submit a final offer? 137.132 Section 137.132 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES....132 How does the Indian Tribe submit a final offer? (a) A written final offer should be submitted: (1...

  14. 42 CFR 137.132 - How does the Indian Tribe submit a final offer?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false How does the Indian Tribe submit a final offer? 137.132 Section 137.132 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES....132 How does the Indian Tribe submit a final offer? (a) A written final offer should be submitted: (1...

  15. 40 CFR 60.2625 - When must I submit my waste management plan?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 6 2011-07-01 2011-07-01 false When must I submit my waste management... or Before November 30, 1999 Model Rule-Waste Management Plan § 60.2625 When must I submit my waste management plan? You must submit a waste management plan no later than the date specified in table 1 of this...

  16. 49 CFR 579.6 - Address for submitting reports and other information.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ..., submissions under subpart B of this part must be submitted to [email protected]gov and submissions under § 579.5 must be submitted to [email protected]gov. (b)(1) Information, documents and reports that are submitted to NHTSA... identified on NHTSA's Web page http://www-odi.nhtsa.dot.gov/ewr/ewr.cfm. A manufacturer shall use the...

  17. 21 CFR 803.13 - Do I need to submit reports in English?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Do I need to submit reports in English? 803.13... in English? (a) Yes. You must submit all written or electronic equivalent reports required by this part in English. (b) If you submit any reports required by this part in an electronic medium, that...

  18. 30 CFR 250.192 - What reports and statistics must I submit relating to a hurricane, earthquake, or other natural...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What reports and statistics must I submit... statistics must I submit relating to a hurricane, earthquake, or other natural occurrence? (a) You must submit evacuation statistics to the Regional Supervisor for a natural occurrence, such as a hurricane, a...

  19. 34 CFR 86.5 - What are the consequences if an IHE fails to submit a drug prevention program certification?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 1 2011-07-01 2011-07-01 false What are the consequences if an IHE fails to submit a drug prevention program certification? 86.5 Section 86.5 Education Office of the Secretary, Department... fails to submit a drug prevention program certification? (a) An IHE that fails to submit a drug...

  20. 34 CFR 86.5 - What are the consequences if an IHE fails to submit a drug prevention program certification?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 34 Education 1 2014-07-01 2014-07-01 false What are the consequences if an IHE fails to submit a drug prevention program certification? 86.5 Section 86.5 Education Office of the Secretary, Department... fails to submit a drug prevention program certification? (a) An IHE that fails to submit a drug...

  1. 43 CFR 3835.92 - What if I fail to submit a timely waiver request?

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 43 Public Lands: Interior 2 2011-10-01 2011-10-01 false What if I fail to submit a timely waiver... MAINTENANCE FEES Defective Waivers and FLPMA Filings § 3835.92 What if I fail to submit a timely waiver request? (a) If you fail to submit a qualified waiver request (see § 3835.1) and also fail to pay an...

  2. 43 CFR 3835.92 - What if I fail to submit a timely waiver request?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false What if I fail to submit a timely waiver... MAINTENANCE FEES Defective Waivers and FLPMA Filings § 3835.92 What if I fail to submit a timely waiver request? (a) If you fail to submit a qualified waiver request (see § 3835.1) and also fail to pay an...

  3. 34 CFR 86.5 - What are the consequences if an IHE fails to submit a drug prevention program certification?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 34 Education 1 2012-07-01 2012-07-01 false What are the consequences if an IHE fails to submit a drug prevention program certification? 86.5 Section 86.5 Education Office of the Secretary, Department... fails to submit a drug prevention program certification? (a) An IHE that fails to submit a drug...

  4. 34 CFR 86.5 - What are the consequences if an IHE fails to submit a drug prevention program certification?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 1 2010-07-01 2010-07-01 false What are the consequences if an IHE fails to submit a drug prevention program certification? 86.5 Section 86.5 Education Office of the Secretary, Department... fails to submit a drug prevention program certification? (a) An IHE that fails to submit a drug...

  5. 34 CFR 86.5 - What are the consequences if an IHE fails to submit a drug prevention program certification?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 34 Education 1 2013-07-01 2013-07-01 false What are the consequences if an IHE fails to submit a drug prevention program certification? 86.5 Section 86.5 Education Office of the Secretary, Department... fails to submit a drug prevention program certification? (a) An IHE that fails to submit a drug...

  6. 43 CFR 3835.92 - What if I fail to submit a timely waiver request?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false What if I fail to submit a timely waiver... MAINTENANCE FEES Defective Waivers and FLPMA Filings § 3835.92 What if I fail to submit a timely waiver request? (a) If you fail to submit a qualified waiver request (see § 3835.1) and also fail to pay an...

  7. 43 CFR 3835.92 - What if I fail to submit a timely waiver request?

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false What if I fail to submit a timely waiver... MAINTENANCE FEES Defective Waivers and FLPMA Filings § 3835.92 What if I fail to submit a timely waiver request? (a) If you fail to submit a qualified waiver request (see § 3835.1) and also fail to pay an...

  8. 14 CFR 193.5 - How may I submit safety or security information and have it protected from disclosure?

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... SUBMITTED INFORMATION § 193.5 How may I submit safety or security information and have it protected from... 14 Aeronautics and Space 3 2011-01-01 2011-01-01 false How may I submit safety or security information and have it protected from disclosure? 193.5 Section 193.5 Aeronautics and Space FEDERAL AVIATION...

  9. 25 CFR 516.2 - When may a person to whom this part applies give testimony, make a statement or submit to interview?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... testimony, make a statement or submit to interview? 516.2 Section 516.2 Indians NATIONAL INDIAN GAMING... whom this part applies give testimony, make a statement or submit to interview? (a) No person to whom... regulation, shall provide testimony, make a statement or submit to interview. (b) Whenever a subpoena...

  10. 14 CFR 193.5 - How may I submit safety or security information and have it protected from disclosure?

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... SUBMITTED INFORMATION § 193.5 How may I submit safety or security information and have it protected from... 14 Aeronautics and Space 3 2010-01-01 2010-01-01 false How may I submit safety or security information and have it protected from disclosure? 193.5 Section 193.5 Aeronautics and Space FEDERAL AVIATION...

  11. 30 CFR 285.607 - How do I submit my SAP?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false How do I submit my SAP? 285.607 Section 285.607... Assessment Plan and Information Requirements for Commercial Leases § 285.607 How do I submit my SAP? You must submit one paper copy and one electronic version of your SAP to MMS at the address listed in § 285.110(a). ...

  12. 76 FR 55577 - Findings of Failure To Submit a Complete State Implementation Plan for Section 110(a) Pertaining...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-08

    ... Failure To Submit a Complete State Implementation Plan for Section 110(a) Pertaining to the 2006 Fine... submission for some or all of these specific requirements. The finding of failure to submit for some or all... making a finding of failure to submit SIPs, or elements of SIPs, required by the CAA, where states have...

  13. 21 CFR 1.279 - When must prior notice be submitted to FDA?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 1 2014-04-01 2014-04-01 false When must prior notice be submitted to FDA? 1.279... Imported Food § 1.279 When must prior notice be submitted to FDA? (a) Except as provided in paragraph (c) of this section, you must submit the prior notice to FDA and the prior notice submission must be...

  14. 21 CFR 1.279 - When must prior notice be submitted to FDA?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 1 2011-04-01 2011-04-01 false When must prior notice be submitted to FDA? 1.279... Imported Food § 1.279 When must prior notice be submitted to FDA? (a) Except as provided in paragraph (c) of this section, you must submit the prior notice to FDA and the prior notice submission must be...

  15. 21 CFR 1.279 - When must prior notice be submitted to FDA?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 1 2012-04-01 2012-04-01 false When must prior notice be submitted to FDA? 1.279... Imported Food § 1.279 When must prior notice be submitted to FDA? (a) Except as provided in paragraph (c) of this section, you must submit the prior notice to FDA and the prior notice submission must be...

  16. 21 CFR 1.279 - When must prior notice be submitted to FDA?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 1 2010-04-01 2010-04-01 false When must prior notice be submitted to FDA? 1.279... Imported Food § 1.279 When must prior notice be submitted to FDA? (a) Except as provided in paragraph (c) of this section, you must submit the prior notice to FDA and the prior notice submission must be...

  17. 21 CFR 1.279 - When must prior notice be submitted to FDA?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 1 2013-04-01 2013-04-01 false When must prior notice be submitted to FDA? 1.279... Imported Food § 1.279 When must prior notice be submitted to FDA? (a) Except as provided in paragraph (c) of this section, you must submit the prior notice to FDA and the prior notice submission must be...

  18. Requesting Different Nodes Types When Submitting Jobs on the Peregrine

    Science.gov Websites

    System | High-Performance Computing | NREL Requesting Different Nodes Types When Submitting Jobs on the Peregrine System Requesting Different Nodes Types When Submitting Jobs on the Peregrine

  19. PrimateLit Database: Submit Literature for Indexing

    Science.gov Websites

    Access PrimateLit Using this Site About the Project Submit Literature for Indexing Copyright Info Center WI Regional Primate Resource Center Submit Literature for Indexing PrimateLit has not been

  20. Facts about Microcephaly

    MedlinePlus

    ... input class="button submit" name="commit" type="submit" value="Submit" /> Information For… Media Policy ... and Severe Microcephaly Comparison The images are in the public domain and thus free of any copyright restrictions. As a matter of ...

  1. 40 CFR 52.1322 - Original Identification of Plan Section.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... listed below were submitted on the dates specified. (1) Budget and manpower projections were submitted by... analysis of those measures, and the results of the carbon monoxide dispersion modeling, submitted on...

  2. 40 CFR 52.1322 - Original Identification of Plan Section.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... listed below were submitted on the dates specified. (1) Budget and manpower projections were submitted by... analysis of those measures, and the results of the carbon monoxide dispersion modeling, submitted on...

  3. 40 CFR 52.1322 - Original Identification of Plan Section.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... listed below were submitted on the dates specified. (1) Budget and manpower projections were submitted by... analysis of those measures, and the results of the carbon monoxide dispersion modeling, submitted on...

  4. 40 CFR 52.1322 - Original Identification of Plan Section.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... listed below were submitted on the dates specified. (1) Budget and manpower projections were submitted by... analysis of those measures, and the results of the carbon monoxide dispersion modeling, submitted on...

  5. 40 CFR 52.1322 - Original Identification of Plan Section.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... listed below were submitted on the dates specified. (1) Budget and manpower projections were submitted by... analysis of those measures, and the results of the carbon monoxide dispersion modeling, submitted on...

  6. Top predators negate the effect of mesopredators on prey physiology.

    PubMed

    Palacios, Maria M; Killen, Shaun S; Nadler, Lauren E; White, James R; McCormick, Mark I

    2016-07-01

    Predation theory and empirical evidence suggest that top predators benefit the survival of resource prey through the suppression of mesopredators. However, whether such behavioural suppression can also affect the physiology of resource prey has yet to be examined. Using a three-tier reef fish food web and intermittent-flow respirometry, our study examined changes in the metabolic rate of resource prey exposed to combinations of mesopredator and top predator cues. Under experimental conditions, the mesopredator (dottyback, Pseudochromis fuscus) continuously foraged and attacked resource prey (juveniles of the damselfish Pomacentrus amboinensis) triggering an increase in prey O2 uptake by 38 ± 12·9% (mean ± SE). The visual stimulus of a top predator (coral trout, Plectropomus leopardus) restricted the foraging activity of the mesopredator, indirectly allowing resource prey to minimize stress and maintain routine O2 uptake. Although not as strong as the effect of the top predator, the sight of a large non-predator species (thicklip wrasse, Hemigymnus melapterus) also reduced the impact of the mesopredator on prey metabolic rate. We conclude that lower trophic-level species can benefit physiologically from the presence of top predators through the behavioural suppression that top predators impose on mesopredators. By minimizing the energy spent on mesopredator avoidance and the associated stress response to mesopredator attacks, prey may be able to invest more energy in foraging and growth, highlighting the importance of the indirect, non-consumptive effects of top predators in marine food webs. © 2016 The Authors. Journal of Animal Ecology published by John Wiley & Sons Ltd on behalf of British Ecological Society.

  7. Intestinal microbiota composition is altered according to nutritional biorhythms in the leopard coral grouper (Plectropomus leopardus).

    PubMed

    Mekuchi, Miyuki; Asakura, Taiga; Sakata, Kenji; Yamaguchi, Tomofumi; Teruya, Kazuhisa; Kikuchi, Jun

    2018-01-01

    Aquaculture is currently a major source of fish and has the potential to become a major source of protein in the future. These demands require efficient aquaculture. The intestinal microbiota plays an integral role that benefits the host, providing nutrition and modulating the immune system. Although our understanding of microbiota in fish gut has increased, comprehensive studies examining fish microbiota and host metabolism remain limited. Here, we investigated the microbiota and host metabolism in the coral leopard grouper, which is traded in Asian markets as a superior fish and has begun to be produced via aquaculture. We initially examined the structural changes of the gut microbiota using next-generation sequencing and found that the composition of microbiota changed between fasting and feeding conditions. The dominant phyla were Proteobacteria in fasting and Firmicutes in feeding; interchanging the dominant bacteria required 12 hours. Moreover, microbiota diversity was higher under feeding conditions than under fasting conditions. Multivariate analysis revealed that Proteobacteria are the key bacteria in fasting and Firmicutes and Fusobacteria are the key bacteria in feeding. Subsequently, we estimated microbiota functional capacity. Microbiota functional structure was relatively stable throughout the experiment; however, individual function activity changed according to feeding conditions. Taken together, these findings indicate that the gut microbiota could be a key factor to understanding fish feeding conditions and play a role in interactions with host metabolism. In addition, the composition of microbiota in ambient seawater directly affects the fish; therefore, it is important to monitor the microbiota in rearing tanks and seawater circulating systems.

  8. Species-Specific Responses of Carnivores to Human-Induced Landscape Changes in Central Argentina

    PubMed Central

    Caruso, Nicolás; Lucherini, Mauro; Fortin, Daniel; Casanave, Emma B.

    2016-01-01

    The role that mammalian carnivores play in ecosystems can be deeply altered by human-driven habitat disturbance. While most carnivore species are negatively affected, the impact of habitat changes is expected to depend on their ecological flexibility. We aimed to identify key factors affecting the habitat use by four sympatric carnivore species in landscapes of central Argentina. Camera trapping surveys were carried out at 49 sites from 2011 to 2013. Each site was characterized by 12 habitat attributes, including human disturbance and fragmentation. Four landscape gradients were created from Principal Component Analysis and their influence on species-specific habitat use was studied using Generalized Linear Models. We recorded 74 events of Conepatus chinga, 546 of Pseudalopex gymnocercus, 193 of Leopardus geoffroyi and 45 of Puma concolor. We found that the gradient describing sites away from urban settlements and with low levels of disturbance had the strongest influence. L. geoffroyi was the only species responding significantly to the four gradients and showing a positive response to modified habitats, which could be favored by the low level of persecution by humans. P. concolor made stronger use of most preserved sites with low proportion of cropland, even though the species also used sites with an intermediate level of fragmentation. A more flexible use of space was found for C. chinga and P. gymnocercus. Our results demonstrate that the impact of human activities spans across this guild of carnivores and that species-specific responses appear to be mediated by ecological and behavioral attributes. PMID:26950300

  9. Survey of ciguatera at Enewetak and Bikini, Marshall Islands, with notes on the systematics and food habits of ciguatoxic fishes

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Randall, J.E.

    1980-04-01

    A total of 551 specimens of 48 species of potentially ciguatoxic fishes from Enewetak and 256 specimens of 23 species from Bikini, Marshall Islands, were tested for ciguatoxin by feeding liver or liver and viscera from these fishes to mongooses at 10% body weight (except for sharks, when only muscle tissue was used). The fishes are representatives of the following families: Orectolobidae, Carcharhinidae, Dasyatidae, Muraenidae, Holocentridae, Sphyraenidae, Mugilidae, Serranidae, Lutjanidae, Lethrinidae, Carangidae, Scombridae, Labridae, Scaridae, Acanthuridae, and Balistidae. The species selected were all ones for which toxicity can be expected, including the worst offenders from reports of ciguatera throughout Oceania;more » only moderate to large-sized adults were tested. In all, 37.3% of the fishes from Enewetak and 19.7% from Bikini gave a positive reaction for ciguatoxin. Because liver and other viscera are more toxic than muscle, the percentage of positive reactions at the level which might cause illness in humans eating only the flesh of these fishes collectively would drop to 16.2 for Enewetak and 1.4 for Bikini. This level of toxicity is not regarded as high for Pacific islands, in general. Because ciguatoxin is acquired through feeding, the food habits of these fishes were investigated. Most of the highly toxic species, including seven of the eight causing severe illness or death in the test animals (Lycodontis javanicus, Cephalopholis argus, Epinephelus hoedtii, E. microdon, Plectropomus leopardus, Aprion virescens, and Lutjanus bohar) are primarily piscivorous.« less

  10. 21 CFR 803.53 - If I am a manufacturer, in which circumstances must I submit a 5-day report?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... must I submit a 5-day report? 803.53 Section 803.53 Food and Drugs FOOD AND DRUG ADMINISTRATION... Reporting Requirements § 803.53 If I am a manufacturer, in which circumstances must I submit a 5-day report? You must submit a 5-day report to us, on Form 3500A or an electronic equivalent approved under § 803...

  11. 21 CFR 803.53 - If I am a manufacturer, in which circumstances must I submit a 5-day report?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... must I submit a 5-day report? 803.53 Section 803.53 Food and Drugs FOOD AND DRUG ADMINISTRATION... Reporting Requirements § 803.53 If I am a manufacturer, in which circumstances must I submit a 5-day report? You must submit a 5-day report to us, on Form 3500A or an electronic equivalent approved under § 803...

  12. 21 CFR 803.53 - If I am a manufacturer, in which circumstances must I submit a 5-day report?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... must I submit a 5-day report? 803.53 Section 803.53 Food and Drugs FOOD AND DRUG ADMINISTRATION... Reporting Requirements § 803.53 If I am a manufacturer, in which circumstances must I submit a 5-day report? You must submit a 5-day report to us, on Form 3500A or an electronic equivalent approved under § 803...

  13. 21 CFR 803.53 - If I am a manufacturer, in which circumstances must I submit a 5-day report?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... must I submit a 5-day report? 803.53 Section 803.53 Food and Drugs FOOD AND DRUG ADMINISTRATION... Reporting Requirements § 803.53 If I am a manufacturer, in which circumstances must I submit a 5-day report? You must submit a 5-day report to us, on Form 3500A or an electronic equivalent approved under § 803...

  14. 13 CFR 126.613 - How does a price evaluation preference affect the bid of a qualified HUBZone SBC in full and open...

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... full and open competition, a qualified HUBZone SBC submits an offer of $98, a non-HUBZone SBC submits..., the non-HUBZone SBC's offer at $100 does not displace the large business' offer because a price... and open competition, a qualified HUBZone SBC submits an offer of $98 and a non-HUBZone SBC submits an...

  15. 44 CFR 65.3 - Requirement to submit new technical data.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... available, a community shall notify the Administrator of the changes by submitting technical or scientific... IDENTIFICATION AND MAPPING OF SPECIAL HAZARD AREAS § 65.3 Requirement to submit new technical data. A community's...

  16. 30 CFR 285.622 - How do I submit my COP?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... ALTERNATE USES OF EXISTING FACILITIES ON THE OUTER CONTINENTAL SHELF Plans and Information Requirements Construction and Operations Plan for Commercial Leases § 285.622 How do I submit my COP? (a) You must submit...

  17. 78 FR 25279 - Agency Information Collection Activities; Proposed Collection; Comment Request; Abbreviated New...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-30

    ... abbreviated new animal drug applications submitted to the Center for Veterinary Medicine, FDA. DATES: Submit....) Although over the last 5 fiscal years all sponsors chose to submit traditional ANADAs, some sponsors did...

  18. A Checklist for Submitting Your Risk Management Plan (RMP)

    EPA Pesticide Factsheets

    Important information about 2014 submissions and a checklist to consider in preparing and resubmitting a 5-year update, as required by 40 CFR part 68. Use the RMP*eSubmit software application, which replaced RMP*Submit.

  19. 78 FR 12759 - Draft Guidance for Industry on Attachment to Guidance on Antiviral Product Development-Conducting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-25

    ...--Conducting and Submitting Virology Studies to the Agency: Guidance for Submitting Hepatitis C Virus... sponsors in submitting hepatitis C virus (HCV) clinical virology data, which are important for supporting...

  20. 21 CFR 822.20 - What are the consequences if I fail to submit a postmarket surveillance plan, my plan is...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false What are the consequences if I fail to submit a postmarket surveillance plan, my plan is disapproved and I fail to submit a new plan, or I fail to conduct... Review and Action § 822.20 What are the consequences if I fail to submit a postmarket surveillance plan...

  1. 21 CFR 822.20 - What are the consequences if I fail to submit a postmarket surveillance plan, my plan is...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false What are the consequences if I fail to submit a postmarket surveillance plan, my plan is disapproved and I fail to submit a new plan, or I fail to conduct... Review and Action § 822.20 What are the consequences if I fail to submit a postmarket surveillance plan...

  2. 21 CFR 822.20 - What are the consequences if I fail to submit a postmarket surveillance plan, my plan is...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false What are the consequences if I fail to submit a postmarket surveillance plan, my plan is disapproved and I fail to submit a new plan, or I fail to conduct... Review and Action § 822.20 What are the consequences if I fail to submit a postmarket surveillance plan...

  3. 21 CFR 822.20 - What are the consequences if I fail to submit a postmarket surveillance plan, my plan is...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false What are the consequences if I fail to submit a postmarket surveillance plan, my plan is disapproved and I fail to submit a new plan, or I fail to conduct... Review and Action § 822.20 What are the consequences if I fail to submit a postmarket surveillance plan...

  4. 21 CFR 822.20 - What are the consequences if I fail to submit a postmarket surveillance plan, my plan is...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false What are the consequences if I fail to submit a postmarket surveillance plan, my plan is disapproved and I fail to submit a new plan, or I fail to conduct... Review and Action § 822.20 What are the consequences if I fail to submit a postmarket surveillance plan...

  5. 21 CFR 803.53 - If I am a manufacturer, in which circumstances must I submit a 5-day report?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., including any trend analysis; or (b) We have made a written request for the submission of a 5-day report. If... must I submit a 5-day report? 803.53 Section 803.53 Food and Drugs FOOD AND DRUG ADMINISTRATION... must I submit a 5-day report? You must submit a 5-day report to us, on Form 3500A or an electronic...

  6. 48 CFR 970.5223-7 - Sustainable acquisition program.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... coordinate its activities with and submit required reports through the Environmental Sustainability... clause, the Contractor shall coordinate its activities with and submit required reports through the Environmental Sustainability Coordinator or equivalent position. (g) The Contractor shall prepare and submit...

  7. 20 CFR 661.430 - Under what conditions may the Governor submit a Workforce Flexibility Plan?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... ADMINISTRATION, DEPARTMENT OF LABOR STATEWIDE AND LOCAL GOVERNANCE OF THE WORKFORCE INVESTMENT SYSTEM UNDER TITLE... Plan or may be submitted separately. If it is submitted separately, the workforce flexibility plan must...

  8. 78 FR 72078 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; NSPS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-02

    ... Request Submitted to OMB for Review and Approval; Comment Request; NSPS for Phosphate Rock Plants (Renewal... Agency has submitted an information collection request (ICR), ``NSPS for Phosphate Rock Plants (40 CFR... 72079

  9. 40 CFR 60.2795 - In what form can I submit my reports?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... (CDX) by using the Electronic Reporting Tool (ERT) (see http://www.epa.gov/ttn/chief/ert/ert_tool.html). ... Reporting § 60.2795 In what form can I submit my reports? Submit initial, annual, and deviation reports...

  10. 76 FR 67729 - Information Collection Being Submitted for Review and Approval to the Office of Management and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-02

    ... operators submit this form to local franchising authorities or the Commission, in situations where the FCC... changes in external costs. Cable operators submit FCC Form 1240 to their respective local franchising...

  11. 78 FR 70933 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-27

    ...: Catalina Solar, LLC. Description: Catalina Solar, LLC submits Catalina Solar Notice of Effective Date of.... Description: Catalina Solar, LLC submits Catalina Solar Notice of Certificate of Concurrence Effective...: Catalina Solar Lessee, LLC submits Catalina Solar Lessee Notice of Certificate of Concurrence Effective...

  12. Filing a Pre-manufacture Notice with EPA

    EPA Pesticide Factsheets

    A pre-manufacture notice, or PMN, must be submitted to EPA at least 90 days prior to the manufacture or import of the new chemical. Here you can learn about the information needed to submit a PMN, and what to expect after you submit.

  13. General RMP Guidance - Chapter 9: Risk Management Plan

    EPA Pesticide Factsheets

    You must submit one risk management plan (RMP) to EPA for all of your covered processes. The internet-based RMP*eSubmit allows you to submit your RMP in EPA's Central Data Exchange (CDX), where you can access and change/correct existing RMPs.

  14. Expansion and Automation of the Energy Conserving Orientational Force for Calculation of Grain Boundary Mobility

    DTIC Science & Technology

    2018-02-21

    change. Approved for public release; distribution is unlimited. 18 A.1 ThermalData.py import numpy import math import os from submit_maker...numpy import math import os from submit_maker import * import time from decimal import * #NOTE: ThermalData.py for the given material and...8217/’+Orientation+’/Submitfiles/submit_excal_’+r epr(temp)+’K_’+repr(u)+’eV.bash’) A.3 submit_maker.py import math import os def

  15. Emerging Trends in the Volume and Format of Outside Examinations Submitted for Secondary Interpretation

    PubMed Central

    Hunt, Christopher H.; Wood, Christopher P.; Diehn, Felix E.; Eckel, Laurence J.; Schwartz, Kara M.; Erickson, Bradley J.

    2014-01-01

    OBJECTIVE The purpose of this article is to describe the trends of secondary interpretations, including the total volume and format of cases. MATERIALS AND METHODS This retrospective study involved all outside neuroradiology examinations submitted for secondary interpretation from November 2006 through December 2010. This practice utilizes consistent criteria and includes all images that cover the brain, neck, and spine. For each month, the total number of outside examinations and their format (i.e., hard-copy film, DICOM CD-ROM, or non-DICOM CD-ROM) were recorded. RESULTS There was no significant change in the volume of cases (1043 ± 131 cases/month; p = 0.46, two-sided Student t test). There was a significant decrease in the volume of hard-copy films submitted, with the mean number of examinations submitted per month on hard-copy film declining from 297 in 2007 to 57 in 2010 (p < 0.0001, Student t test). This decrease was mirrored by an increase in the mean number of cases submitted on CD-ROM (753 cases/month in 2007 and 1036 cases/month in 2010; p < 0.0001). Although most were submitted in DICOM format, there was almost a doubling of the volume of cases submitted on non-DICOM CD-ROM (mean number of non-DICOM CD-ROMs, nine cases/month in 2007 and 17 cases/month in 2010; p < 0.001). CONCLUSION There has been a significant decrease in the number of hard-copy films submitted for secondary interpretation. There has been almost a doubling of the volume of cases submitted in non-DICOM formats, which is unfortunate, given the many advantages of the internationally derived DICOM standard, including ease of archiving, standardized display, efficient review, improved interpretation, and quality of patient care. PMID:22451538

  16. Emerging trends in the volume and format of outside examinations submitted for secondary interpretation.

    PubMed

    Hunt, Christopher H; Wood, Christopher P; Diehn, Felix E; Eckel, Laurence J; Schwartz, Kara M; Erickson, Bradley J

    2012-04-01

    The purpose of this article is to describe the trends of secondary interpretations, including the total volume and format of cases. This retrospective study involved all outside neuroradiology examinations submitted for secondary interpretation from November 2006 through December 2010. This practice utilizes consistent criteria and includes all images that cover the brain, neck, and spine. For each month, the total number of outside examinations and their format (i.e., hard-copy film, DICOM CD-ROM, or non-DICOM CD-ROM) were recorded. There was no significant change in the volume of cases (1043 ± 131 cases/month; p = 0.46, two-sided Student t test). There was a significant decrease in the volume of hard-copy films submitted, with the mean number of examinations submitted per month on hard-copy film declining from 297 in 2007 to 57 in 2010 (p < 0.0001, Student t test). This decrease was mirrored by an increase in the mean number of cases submitted on CD-ROM (753 cases/month in 2007 and 1036 cases/month in 2010; p < 0.0001). Although most were submitted in DICOM format, there was almost a doubling of the volume of cases submitted on non-DICOM CD-ROM (mean number of non-DICOM CD-ROMs, nine cases/month in 2007 and 17 cases/month in 2010; p < 0.001). There has been a significant decrease in the number of hard-copy films submitted for secondary interpretation. There has been almost a doubling of the volume of cases submitted in non-DICOM formats, which is unfortunate, given the many advantages of the internationally derived DICOM standard, including ease of archiving, standardized display, efficient review, improved interpretation, and quality of patient care.

  17. 30 CFR 1210.201 - How do I submit Form MMS-4430, Solid Minerals Production and Royalty Report?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... submitting Form MMS-4430 are available on our Internet reporting web site or you may contact us toll free at... submit Form MMS-4430 electronically using our Internet reporting web site unless you meet the conditions...

  18. 45 CFR 144.206 - Reporting requirements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...-term care insurance partnership must submit, in accordance with the requirements of this section, data... care insurance policies must submit the following data to the Secretary by the deadlines specified in... certificates. (i) Insurers must submit data on— (A) Any insured individual who held an active partnership...

  19. 29 CFR 1956.21 - Procedures for submitting changes.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 9 2010-07-01 2010-07-01 false Procedures for submitting changes. 1956.21 Section 1956.21 Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION..., Change, Evaluation and Withdrawal of Approval Procedures § 1956.21 Procedures for submitting changes. The...

  20. 5 CFR 1605.14 - Misclassified retirement system coverage.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... is a FERCCA correction, the employing agency must submit makeup employee contributions on late... correction, the employing agency must submit makeup employee contributions on current payment records; in such cases, the employee is not entitled to breakage. Agency makeup contributions may be submitted on...

  1. 78 FR 52191 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-22

    ... Submitted to OMB for Review and Approval; Comment Request; Reformulated Gasoline Commingling Provisions... Protection Agency has submitted an information collection request (ICR), Reformulated Gasoline Commingling...: EPA would like to continue collecting notifications from gasoline retailers and wholesale purchaser...

  2. 25 CFR 20.204 - Must all tribes submit a tribal redesign plan?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... ASSISTANCE AND SOCIAL SERVICES PROGRAMS Welfare Reform § 20.204 Must all tribes submit a tribal redesign plan? No, you must submit a tribal redesign plan under § 20.206 only if you want to change the way that the...

  3. Ask a SEER Registrar - SEER Registrars

    Cancer.gov

    First submit questions to your central registry as required and they will submit them to SEER. Use the form on this page to submit questions to SEER staff about coding cancer cases or SEER's reporting guideline materials. Coding and abstracting answers are on SEER Inquiry System website.

  4. Mercury in the gold mining district of San Martin de Loba, South of Bolivar (Colombia).

    PubMed

    Olivero-Verbel, Jesus; Caballero-Gallardo, Karina; Turizo-Tapia, Alexi

    2015-04-01

    Gold mining is responsible for most Hg pollution in developing countries. The aims of this study were to assess the levels of total Hg (T-Hg) in human hair, fish, water, macrophyte, and sediment samples in the gold mining district of San Martin de Loba, Colombia, as well as to determine fish consumption-based risks for T-Hg ingestion. T-Hg levels were measured by electrothermal atomization and atomic absorption spectroscopy. The overall mean T-Hg level in hair for humans in the mining district of San Martin de Loba was 2.12 μg/g, whereas for the reference site, Chimichagua, Cesar, it was 0.58 μg/g. Mean T-Hg levels were not different when considered within localities belonging to the mining district but differed when the comparison included Chimichagua. T-Hg levels in examined locations were weakly but significantly associated with age and height, as well as with fish consumption, except in San Martin de Loba. High T-Hg concentrations in fish were detected in Pseudoplatystoma magdaleniatum, Caquetaia kraussii, Ageneiosus pardalis, Cyrtocharax magdalenae, and Triportheus magdalenae, whereas the lowest appeared in Prochilodus magdalenae and Hemiancistrus wilsoni. In terms of Hg exposure due to fish consumption, only these last two species offer some guarantee of low risk for Hg-related health problems. Water, floating macrophytes, and sediments from effluents near mining sites also had high Hg values. In mines of San Martin de Loba and Hatillo de Loba, for instance, the geoaccumulation index (I(geo)) for sediments reached values greater than 6, indicating extreme pollution. In short, these data support the presence of a high Hg-polluted environment in this mining district, with direct risk for deleterious effects on the health of the mining communities.

  5. Fungal dermatitis, glossitis and disseminated visceral mycosis caused by different Metarhizium granulomatis genotypes in veiled chameleons (Chamaeleo calyptratus) and first isolation in healthy lizards.

    PubMed

    Schmidt, Volker; Klasen, Linus; Schneider, Juliane; Hübel, Jens; Pees, Michael

    2017-08-01

    Metarhizium (M.) granulomatis (formerly Chamaeleomyces granulomatis) invariably causes fatal fungal glossitis and systemic mycosis in veiled chameleons (Chamaeleo calyptratus). Isolation of M. granulomatis in other lizards thus far has not been described. The aim of this study therefore was to obtain information on the presence of M. granulomatis in reptiles kept as pets, and to examine whether there was an association between specific genotypes and clinical/pathological outcomes. Besides 18S ribosomal (r) DNA (SSU) and internal transcribed spacer1-5.8S (ITS1-5.8S) rDNA, a fragment of the large subunit of the 28S rDNA (LSU), including the domains 1 (D1) and D2, were sequenced for identification of the fungus and phylogenetic analysis. Metarhizium granulomatis was isolated from 23 veiled chameleons, two panther chameleons (Furcifer pardalis) and one central bearded dragon (Pogona vitticeps). Only the veiled chameleons revealed corresponding pathological findings in the form of glossal hemorrhage, granulomatous glossitis, pharyngitis, dermatitis and/or visceral mycosis. The infection site correlated to survival times of infected veiled chameleons. Combined long-term treatment with terbinafine and nystatin based on susceptibility testing may be helpful for prevention of disease and visceral spreading of the fungus, but elimination of the fungal pathogen or successful treatment of diseased veiled chameleons have not been achieved yet. Sequencing of the ribosomal genes yielded five different genotypes, with genotype A being strongly correlated with dermatitis, and remaining genotypes with pharyngitis and glossitis. However, disseminated visceral mycosis developed irrespective of the genotypes. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Mercury pollution by gold mining in a global biodiversity hotspot, the Choco biogeographic region, Colombia.

    PubMed

    Palacios-Torres, Yuber; Caballero-Gallardo, Karina; Olivero-Verbel, Jesus

    2018-02-01

    Mercury (Hg) is a harmful pollutant released into the environment from gold mining activities, representing a risk to human health and the ecosystems. The aim of this study was to assess the levels of total Hg (T-Hg) in human hair, fish, sediments and air; and to determine fish consumption-based risks for T-Hg ingestion in the Choco biogeographic region, a global biodiversity hotspot located at the Colombian Pacific. Mercury concentrations in hair were measured in two locations, Quibdo, the state capital, and Paimado, a riverine community. The median T-Hg value in human hair in Quibdo was 1.26 μg/g (range: 0.02-116.40 μg/g), whereas in Paimado it was 0.67 μg/g (range: 0.07-6.47 μg/g). Mercury levels in examined locations were weakly associated with height (ρ = 0.145, P = 0.024). Air T-Hg levels in Quibdo were high inside gold shops being up to 200.9-fold greater than the background. Mercury concentrations in fish from Atrato River were above WHO limit (0.5 μg/g), with highest levels in Pseudopimelodus schultzi, Ageneiosus pardalis, Sternopygus aequilabiatus, Rhamdia quelen and Hoplias malabaricus, whereas the lowest appeared in Cyphocharax magdalenae and Hemiancistrus wilsoni. Based on fish consumption, these last two species offer low risk to human health. Sediment samples from fifty different sites of Atrato River showed low T-Hg concentrations, with little variability between stations. However, contamination factors revealed a moderate pollution in 44% of sampling sites along the river. In conclusion, Hg pollution is widespread in the Biogeographic Choco and governmental actions must be taken to protect the population and preserve its biodiversity. Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. 47 CFR 1.934 - Defective applications and dismissal.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... designated for comparative hearing; or (B) It is an application for which the applicant submitted the winning... has been designated for comparative hearing may submit a written petition requesting that the... submit the winning bid in a competitive bidding process; or (2) That receive comparative consideration in...

  8. 47 CFR 1.934 - Defective applications and dismissal.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... designated for comparative hearing; or (B) It is an application for which the applicant submitted the winning... has been designated for comparative hearing may submit a written petition requesting that the... submit the winning bid in a competitive bidding process; or (2) That receive comparative consideration in...

  9. 28 CFR 54.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 28 Judicial Administration 2 2010-07-01 2010-07-01 false Educational institutions eligible to submit transition plans. 54.225 Section 54.225 Judicial Administration DEPARTMENT OF JUSTICE (CONTINUED... Coverage § 54.225 Educational institutions eligible to submit transition plans. (a) Application. This...

  10. 40 CFR 60.1385 - What reports must I submit after I submit my notice of construction and in what form?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... SOURCES Standards of Performance for Small Municipal Waste Combustion Units for Which Construction is... may submit electronic reports. (c) Keep a copy of all reports required by §§ 60.1400, 60.1410, and 60...

  11. 40 CFR 60.1385 - What reports must I submit after I submit my notice of construction and in what form?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... SOURCES Standards of Performance for Small Municipal Waste Combustion Units for Which Construction is... may submit electronic reports. (c) Keep a copy of all reports required by §§ 60.1400, 60.1410, and 60...

  12. 40 CFR 60.1385 - What reports must I submit after I submit my notice of construction and in what form?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... SOURCES Standards of Performance for Small Municipal Waste Combustion Units for Which Construction is... may submit electronic reports. (c) Keep a copy of all reports required by §§ 60.1400, 60.1410, and 60...

  13. 40 CFR 60.1385 - What reports must I submit after I submit my notice of construction and in what form?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... SOURCES Standards of Performance for Small Municipal Waste Combustion Units for Which Construction is... may submit electronic reports. (c) Keep a copy of all reports required by §§ 60.1400, 60.1410, and 60...

  14. 40 CFR 60.1385 - What reports must I submit after I submit my notice of construction and in what form?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... SOURCES Standards of Performance for Small Municipal Waste Combustion Units for Which Construction is... may submit electronic reports. (c) Keep a copy of all reports required by §§ 60.1400, 60.1410, and 60...

  15. 40 CFR 52.2223 - Compliance schedules.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ..., 1974: Submit to the Administrator a projection of the amount of fuel, by types, that will be... projected above. (iii) April 30, 1974: Submit a statement as to whether boiler modifications will be required. If modifications will be required, submit plans for such modifications. (iv) May 31, 1974: Let...

  16. 75 FR 60404 - Information Collection Request Submitted to Office of Management and Budget

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-30

    ... [OMB Control Number: 3002-0003] Information Collection Request Submitted to Office of Management and... United States will submit an Information Collection Request (ICR) to the Office of Management and Budget (OMB) requesting approval for the following collection of information: 3002-0003, Substitute...

  17. 75 FR 6195 - Combined Notice of Filings # 1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-08

    ... Management Company, LLC. Description: Thornwood Management Co submits revisions to its market-based rate... Management Company, LLC. Description: Thornwood Management Co, LLC submits the Updated Market Power Analysis...: Algonquin Tinker Gen Co et al submits a Notice of Name Change and Succession. Filed Date: 01/27/2010...

  18. 45 CFR 2523.110 - Can Federal agencies submit multiple applications?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Can Federal agencies submit multiple applications? 2523.110 Section 2523.110 Public Welfare Regulations Relating to Public Welfare (Continued) CORPORATION... AMERICORPS PROGRAM ASSISTANCE § 2523.110 Can Federal agencies submit multiple applications? No. The...

  19. 78 FR 43879 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-22

    ... Submitted to OMB for Review and Approval; Comment Request; Standards for Pesticide Containers and... Protection Agency has submitted an information collection request (ICR), ``Standards for Pesticide Containers... covers the information collection activities associated with the container design and residue removal...

  20. 10 CFR 1042.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Educational institutions eligible to submit transition plans. 1042.225 Section 1042.225 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON... § 1042.225 Educational institutions eligible to submit transition plans. (a) Application. This section...

  1. 10 CFR 1042.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 4 2013-01-01 2013-01-01 false Educational institutions eligible to submit transition plans. 1042.225 Section 1042.225 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON... § 1042.225 Educational institutions eligible to submit transition plans. (a) Application. This section...

  2. 10 CFR 1042.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Educational institutions eligible to submit transition plans. 1042.225 Section 1042.225 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON... § 1042.225 Educational institutions eligible to submit transition plans. (a) Application. This section...

  3. 10 CFR 1042.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Educational institutions eligible to submit transition plans. 1042.225 Section 1042.225 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON... § 1042.225 Educational institutions eligible to submit transition plans. (a) Application. This section...

  4. 10 CFR 1042.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Educational institutions eligible to submit transition plans. 1042.225 Section 1042.225 Energy DEPARTMENT OF ENERGY (GENERAL PROVISIONS) NONDISCRIMINATION ON... § 1042.225 Educational institutions eligible to submit transition plans. (a) Application. This section...

  5. 40 CFR 52.2720 - Identification of plan.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... submitted on April 5, 1973, by the Commonwealth of Puerto Rico Environmental Quality Board. (2) Compliance schedules submitted on April 9, 1973, by the Commonwealth of Puerto Rico Environmental Quality Board. (3... Board. (4) Compliance schedules submitted on May 30, 1973, by the Commonwealth of Puerto Rico...

  6. 40 CFR 52.2720 - Identification of plan.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... submitted on April 5, 1973, by the Commonwealth of Puerto Rico Environmental Quality Board. (2) Compliance schedules submitted on April 9, 1973, by the Commonwealth of Puerto Rico Environmental Quality Board. (3... Board. (4) Compliance schedules submitted on May 30, 1973, by the Commonwealth of Puerto Rico...

  7. Prospective Higher Education Providers

    ERIC Educational Resources Information Center

    Australian Government Tertiary Education Quality and Standards Agency, 2016

    2016-01-01

    Since the inception of the Australian Government Tertiary Education Quality and Standards Agency (TEQSA) in January 2012, 106 organisations have submitted or indicated their intention to submit applications for initial registration to TEQSA. Of those who have submitted applications, 2 have been rejected, 10 have subsequently been withdrawn by the…

  8. 42 CFR 102.43 - Deadlines for submitting documentation.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 1 2014-10-01 2014-10-01 false Deadlines for submitting documentation. 102.43 Section 102.43 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES VACCINES SMALLPOX COMPENSATION PROGRAM Procedures for Filing Request Packages § 102.43 Deadlines for submitting...

  9. 75 FR 11162 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-10

    ...; Wildorado Wind, LLC. Description: San Juan Mesa Wind Project, LLC et al. submits the Updated Market Power... Power Marketing, LLC; High Majestic Wind Energy Center, LLC. Description: NextEra Companies submits.... Description: Golden Spread Electric Cooperative, Inc et al. submits an Updated Market Power Analysis. Filed...

  10. 42 CFR 102.43 - Deadlines for submitting documentation.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 42 Public Health 1 2011-10-01 2011-10-01 false Deadlines for submitting documentation. 102.43 Section 102.43 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES VACCINES SMALLPOX COMPENSATION PROGRAM Procedures for Filing Request Packages § 102.43 Deadlines for submitting...

  11. 42 CFR 102.43 - Deadlines for submitting documentation.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 1 2012-10-01 2012-10-01 false Deadlines for submitting documentation. 102.43 Section 102.43 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES VACCINES SMALLPOX COMPENSATION PROGRAM Procedures for Filing Request Packages § 102.43 Deadlines for submitting...

  12. 42 CFR 102.43 - Deadlines for submitting documentation.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 1 2013-10-01 2013-10-01 false Deadlines for submitting documentation. 102.43 Section 102.43 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES VACCINES SMALLPOX COMPENSATION PROGRAM Procedures for Filing Request Packages § 102.43 Deadlines for submitting...

  13. 42 CFR 102.43 - Deadlines for submitting documentation.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 1 2010-10-01 2010-10-01 false Deadlines for submitting documentation. 102.43 Section 102.43 Public Health PUBLIC HEALTH SERVICE, DEPARTMENT OF HEALTH AND HUMAN SERVICES VACCINES SMALLPOX COMPENSATION PROGRAM Procedures for Filing Request Packages § 102.43 Deadlines for submitting...

  14. 75 FR 69429 - Combined Notice of Filings #1

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-12

    ... Sound Energy, Inc. Description: Puget Sound Energy, Inc. submits tariff filing per 35.12: PSE Original...: ER11-2008-000. Applicants: Puget Sound Energy, Inc. Description: Puget Sound Energy, Inc. submits... Sound Energy, Inc. Description: Puget Sound Energy, Inc. submits tariff filing per 35.12: PSE Original...

  15. 78 FR 75342 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; NESHAP...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-11

    ... Request Submitted to OMB for Review and Approval; Comment Request; NESHAP for Lime Manufacturing (Renewal... Agency has submitted an information collection request (ICR), ``NESHAP for Lime Manufacturing (40 CFR... required semiannually. Form Numbers: None. Respondents/affected entities: Lime manufacturing plants...

  16. 29 CFR 1626.5 - Where to submit complaints and charges.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 Labor 4 2012-07-01 2012-07-01 false Where to submit complaints and charges. 1626.5 Section 1626.5 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES-AGE DISCRIMINATION IN EMPLOYMENT ACT § 1626.5 Where to submit complaints and charges. Complaints and...

  17. 29 CFR 1626.5 - Where to submit complaints and charges.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 29 Labor 4 2013-07-01 2013-07-01 false Where to submit complaints and charges. 1626.5 Section 1626.5 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES-AGE DISCRIMINATION IN EMPLOYMENT ACT § 1626.5 Where to submit complaints and charges. Complaints and...

  18. 29 CFR 1626.5 - Where to submit complaints and charges.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 29 Labor 4 2010-07-01 2010-07-01 false Where to submit complaints and charges. 1626.5 Section 1626.5 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES-AGE DISCRIMINATION IN EMPLOYMENT ACT § 1626.5 Where to submit complaints and charges. Complaints and...

  19. 29 CFR 1626.5 - Where to submit complaints and charges.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 29 Labor 4 2011-07-01 2011-07-01 false Where to submit complaints and charges. 1626.5 Section 1626.5 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES-AGE DISCRIMINATION IN EMPLOYMENT ACT § 1626.5 Where to submit complaints and charges. Complaints and...

  20. 29 CFR 1626.5 - Where to submit complaints and charges.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 Labor 4 2014-07-01 2014-07-01 false Where to submit complaints and charges. 1626.5 Section 1626.5 Labor Regulations Relating to Labor (Continued) EQUAL EMPLOYMENT OPPORTUNITY COMMISSION PROCEDURES-AGE DISCRIMINATION IN EMPLOYMENT ACT § 1626.5 Where to submit complaints and charges. Complaints and...

  1. 49 CFR 551.63 - May a foreign manufacturer submit a designation by email or facsimile?

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... email or facsimile? 551.63 Section 551.63 Transportation Other Regulations Relating to Transportation... foreign manufacturer submit a designation by email or facsimile? No, the statute requires designation... designation documents submitted via email or facsimile, as they do not satisfy this requirement. ...

  2. 18 CFR 154.302 - Previously submitted material.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 18 Conservation of Power and Water Resources 1 2014-04-01 2014-04-01 false Previously submitted material. 154.302 Section 154.302 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY... Filed With Changes § 154.302 Previously submitted material. (a) If all, or any portion, of the...

  3. 18 CFR 154.302 - Previously submitted material.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 18 Conservation of Power and Water Resources 1 2012-04-01 2012-04-01 false Previously submitted material. 154.302 Section 154.302 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY... Filed With Changes § 154.302 Previously submitted material. (a) If all, or any portion, of the...

  4. 18 CFR 154.302 - Previously submitted material.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 18 Conservation of Power and Water Resources 1 2011-04-01 2011-04-01 false Previously submitted material. 154.302 Section 154.302 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY... Filed With Changes § 154.302 Previously submitted material. (a) If all, or any portion, of the...

  5. 18 CFR 154.302 - Previously submitted material.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 18 Conservation of Power and Water Resources 1 2013-04-01 2013-04-01 false Previously submitted material. 154.302 Section 154.302 Conservation of Power and Water Resources FEDERAL ENERGY REGULATORY... Filed With Changes § 154.302 Previously submitted material. (a) If all, or any portion, of the...

  6. 77 FR 10621 - Changes to the In-Bond Process

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-22

    ... submit in-bond applications electronically using a CBP-approved electronic data interchange (EDI) system... electronically submit the in-bond application to CBP via a CBP-approved EDI system. \\6\\ Due to the unique... as the CBP-approved EDI system for submitting the in-bond application and other information that is...

  7. 21 CFR 312.30 - Protocol amendments.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... (b) Changes in a protocol. (1) A sponsor shall submit a protocol amendment describing any change in a Phase 1 protocol that significantly affects the safety of subjects or any change in a Phase 2 or 3... forth the provisions under which new protocols may be submitted and changes in previously submitted...

  8. 21 CFR 312.30 - Protocol amendments.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... (b) Changes in a protocol. (1) A sponsor shall submit a protocol amendment describing any change in a Phase 1 protocol that significantly affects the safety of subjects or any change in a Phase 2 or 3... forth the provisions under which new protocols may be submitted and changes in previously submitted...

  9. 24 CFR 598.300 - Procedure for submitting a nomination.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 3 2014-04-01 2013-04-01 true Procedure for submitting a nomination. 598.300 Section 598.300 Housing and Urban Development Regulations Relating to Housing and Urban... DESIGNATIONS Designation Process § 598.300 Procedure for submitting a nomination. (a) Establishment of...

  10. 24 CFR 598.300 - Procedure for submitting a nomination.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 3 2013-04-01 2013-04-01 false Procedure for submitting a nomination. 598.300 Section 598.300 Housing and Urban Development Regulations Relating to Housing and Urban... DESIGNATIONS Designation Process § 598.300 Procedure for submitting a nomination. (a) Establishment of...

  11. 24 CFR 598.300 - Procedure for submitting a nomination.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 3 2012-04-01 2012-04-01 false Procedure for submitting a nomination. 598.300 Section 598.300 Housing and Urban Development Regulations Relating to Housing and Urban... DESIGNATIONS Designation Process § 598.300 Procedure for submitting a nomination. (a) Establishment of...

  12. 78 FR 54891 - Information Collection Request Submitted to OMB for Review and Approval: Comment Request; Great...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-06

    ... Submitted to OMB for Review and Approval: Comment Request; Great Lakes Accountability System (Reinstatement... Agency has submitted an information collection request (ICR), Great Lakes Accountability System... legislation called for increased accountability for the GLRI and directed EPA to implement a process to track...

  13. 78 FR 66763 - Information Collection: Oil Spill Financial Responsibility for Offshore Facilities; Submitted for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-11-06

    ...] Information Collection: Oil Spill Financial Responsibility for Offshore Facilities; Submitted for OMB Review... information collection request (ICR) concerns the paperwork requirements for 30 CFR 553, Oil Spill Financial... 6, 2013 ADDRESSES: Submit comments on this ICR to the Desk Officer for the Department of the...

  14. 75 FR 32341 - Import Administration IA ACCESS Pilot Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-08

    ... submitted electronically need not also be submitted in hard copy. Persons wishing to submit written comments in hard copy should file one signed original and two copies of each set of comments to the address... . Any questions concerning file formatting, document conversion, access on the Internet, or other...

  15. 78 FR 18576 - Agency Information Collection Activities; Comment Request; Experimental Sites Data Collection...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-27

    ... delivery, or hand delivery. Please note that comments submitted by fax or email and those submitted after the comment period will not be accepted. Written requests for information or comments submitted by... respondents, including through the use of information technology. Please note that written comments received...

  16. 30 CFR 210.51 - Who must submit royalty reports?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Who must submit royalty reports? 210.51 Section 210.51 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT FORMS AND REPORTS Royalty Reports-Oil, Gas, and Geothermal Resources § 210.51 Who must submit...

  17. 10 CFR 600.504 - Information an applicant must submit.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 4 2012-01-01 2012-01-01 false Information an applicant must submit. 600.504 Section 600... Information an applicant must submit. (a) Any applicant for financial assistance under a covered program shall... United States of products resulting from a technology developed with financial assistance or to procure...

  18. 10 CFR 600.504 - Information an applicant must submit.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 4 2014-01-01 2014-01-01 false Information an applicant must submit. 600.504 Section 600... Information an applicant must submit. (a) Any applicant for financial assistance under a covered program shall... United States of products resulting from a technology developed with financial assistance or to procure...

  19. 10 CFR 600.504 - Information an applicant must submit.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 4 2011-01-01 2011-01-01 false Information an applicant must submit. 600.504 Section 600... Information an applicant must submit. (a) Any applicant for financial assistance under a covered program shall... United States of products resulting from a technology developed with financial assistance or to procure...

  20. 28 CFR 542.15 - Appeals.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... response may submit an Appeal on the appropriate form (BP-10) to the appropriate Regional Director within... Regional Director's response may submit an Appeal on the appropriate form (BP-11) to the General Counsel... appeal. (b) Form. (1) Appeals to the Regional Director shall be submitted on the form designed for...

  1. 28 CFR 542.15 - Appeals.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... response may submit an Appeal on the appropriate form (BP-10) to the appropriate Regional Director within... Regional Director's response may submit an Appeal on the appropriate form (BP-11) to the General Counsel... appeal. (b) Form. (1) Appeals to the Regional Director shall be submitted on the form designed for...

  2. 78 FR 52190 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; NESHAP...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-22

    ... Submitted to OMB for Review and Approval; Comment Request; NESHAP for Gasoline Distribution Facilities... Protection Agency has submitted an information collection request (ICR), ``NESHAP for Gasoline Distribution... semiannually at a minimum. Form Numbers: None. Respondents/affected entities: Owners or operators of gasoline...

  3. 27 CFR 73.34 - When is an electronically submitted form considered timely filed?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY (CONTINUED) PROCEDURES AND PRACTICES ELECTRONIC SIGNATURES; ELECTRONIC SUBMISSION OF FORMS Electronic Filing of Documents with TTB § 73.34 When is an electronically submitted form considered timely filed? If you submit a form to our electronic...

  4. 21 CFR 807.37 - Inspection of establishment registration and device listings.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... device listings. 807.37 Section 807.37 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH... Devices and Radiological Health (HFZ-308), Food and Drug Administration, Department of Health and Human...; (ii) All labels submitted; (iii) All labeling submitted; (iv) All advertisements submitted; (v) All...

  5. 40 CFR 98.5 - How is the report submitted?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 20 2010-07-01 2010-07-01 false How is the report submitted? 98.5 Section 98.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING General Provision § 98.5 How is the report submitted? Each GHG report and...

  6. 77 FR 7115 - Committee on Adjudication

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-10

    ... posting comments to the forum and reading comments submitted to the forum by other persons. The public may... posting comments to the forum and reading comments submitted to the forum by other members of the... inappropriate for posting. When submitting comments, please bear in mind that, because the Web forum will be...

  7. 43 CFR 3266.10 - Will BLM disclose information I submit under these regulations?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... public disclosure of data and information contained in Department records. Certain mineral information not protected from disclosure under part 2 of this title may be made available for inspection without... submit under these regulations? All Federal and Indian data and information submitted to the BLM are...

  8. 43 CFR 3255.10 - Will BLM disclose information I submit under these regulations?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... covering public disclosure of data and information contained in Department records. Certain mineral information not protected from disclosure under part 2 may be made available for inspection without a Freedom... submit under these regulations? All Federal and Indian data and information submitted to the BLM are...

  9. 43 CFR 3255.10 - Will BLM disclose information I submit under these regulations?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... covering public disclosure of data and information contained in Department records. Certain mineral information not protected from disclosure under part 2 may be made available for inspection without a Freedom... submit under these regulations? All Federal and Indian data and information submitted to the BLM are...

  10. 43 CFR 3266.10 - Will BLM disclose information I submit under these regulations?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... public disclosure of data and information contained in Department records. Certain mineral information not protected from disclosure under part 2 of this title may be made available for inspection without... submit under these regulations? All Federal and Indian data and information submitted to the BLM are...

  11. 78 FR 26663 - Exelon Generation Company, LLC, License Renewal of Nuclear Plants and Public Meetings for the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-07

    ... and reasonable alternative energy sources. DATES: Submit comments by June 27, 2013. Comments [email protected] . For technical questions, contact the individual(s) listed in the FOR FURTHER INFORMATION... accessing information and submitting comments, see ``Accessing Information and Submitting Comments'' in the...

  12. 77 FR 65715 - Agency Information Collection Activities; Submissions for OMB Review; Comment Request; H-2A...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-30

    ... Prevailing Wage Determination ACTION: Notice. SUMMARY: The Department of Labor (DOL) is submitting four... Certification Program, and Application for Prevailing Wage Determination. DATES: Submit comments on or before... DOL is submitting has been assigned ICR Reference Number 201210-1205-002, Application for Prevailing...

  13. 49 CFR Appendix II to Part 805 - Employees Required To Submit Statements

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...—Employees Required To Submit Statements Statements of employment and financial interests are required of the... 49 Transportation 7 2010-10-01 2010-10-01 false Employees Required To Submit Statements II...) Office of the managing director: (1) Legislative affairs officer. (2) Program analysis officer. (d...

  14. 40 CFR 52.2475 - Approval of plans.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... submitted by the Washington Department of Ecology on September 20, 2001 and November 22, 2004. (ii) EPA... Plan, adopted April 27, 2004 effective June 24, 2004, submitted by the Washington Department of Ecology... Carbon Monoxide Maintenance Plan submitted by the Washington Department of Ecology on April 25, 2007. (ii...

  15. 40 CFR 52.2475 - Approval of plans.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... submitted by the Washington Department of Ecology on September 20, 2001 and November 22, 2004. (ii) EPA... Plan, adopted April 27, 2004 effective June 24, 2004, submitted by the Washington Department of Ecology... Carbon Monoxide Maintenance Plan submitted by the Washington Department of Ecology on April 25, 2007. (ii...

  16. 75 FR 45620 - Combined Notice of Filings #2

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ..., LLC. Description: FPL Energy Oliver Wind I, LLC submits tariff filing per 35.12: Oliver I Baseline... Energy South Dakota Wind, LLC submits tariff filing per 35.12: South Dakota Baseline Filing, to be..., Inc. Description: FPL Energy Stateline II, Inc. submits tariff filing per 35.12: Stateline II Baseline...

  17. 10 CFR 5.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 1 2013-01-01 2013-01-01 false Educational institutions eligible to submit transition plans. 5.225 Section 5.225 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN... institutions eligible to submit transition plans. (a) Application. This section applies to each educational...

  18. 10 CFR 5.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 1 2014-01-01 2014-01-01 false Educational institutions eligible to submit transition plans. 5.225 Section 5.225 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN... institutions eligible to submit transition plans. (a) Application. This section applies to each educational...

  19. 10 CFR 5.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 1 2011-01-01 2011-01-01 false Educational institutions eligible to submit transition plans. 5.225 Section 5.225 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN... institutions eligible to submit transition plans. (a) Application. This section applies to each educational...

  20. 10 CFR 5.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 1 2012-01-01 2012-01-01 false Educational institutions eligible to submit transition plans. 5.225 Section 5.225 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN... institutions eligible to submit transition plans. (a) Application. This section applies to each educational...

  1. 10 CFR 5.225 - Educational institutions eligible to submit transition plans.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 1 2010-01-01 2010-01-01 false Educational institutions eligible to submit transition plans. 5.225 Section 5.225 Energy NUCLEAR REGULATORY COMMISSION NONDISCRIMINATION ON THE BASIS OF SEX IN... institutions eligible to submit transition plans. (a) Application. This section applies to each educational...

  2. 42 CFR 423.2018 - Submitting evidence before the ALJ hearing.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 3 2014-10-01 2014-10-01 false Submitting evidence before the ALJ hearing. 423.2018 Section 423.2018 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND... Reopening, ALJ Hearings, MAC review, and Judicial Review § 423.2018 Submitting evidence before the ALJ...

  3. 42 CFR 423.2018 - Submitting evidence before the ALJ hearing.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 42 Public Health 3 2013-10-01 2013-10-01 false Submitting evidence before the ALJ hearing. 423.2018 Section 423.2018 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND... Reopening, ALJ Hearings, MAC review, and Judicial Review § 423.2018 Submitting evidence before the ALJ...

  4. 42 CFR 423.2018 - Submitting evidence before the ALJ hearing.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 42 Public Health 3 2012-10-01 2012-10-01 false Submitting evidence before the ALJ hearing. 423.2018 Section 423.2018 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND... Reopening, ALJ Hearings, MAC review, and Judicial Review § 423.2018 Submitting evidence before the ALJ...

  5. 76 FR 38585 - Proposed Establishment of Class E Airspace; Wilkes-Barre, PA

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-01

    ... may also submit and review received comments through the Internet at http:[sol][sol]www.regulations... submit comments through the Internet at http:[sol][sol]www.regulations.gov. Annotators wishing the FAA to... downloaded from and comments submitted through http:[sol][sol]www.regulations.gov. Recently published...

  6. 78 FR 56225 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; NESHAP...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-12

    ... Submitted to OMB for Review and Approval; Comment Request; NESHAP for Plywood and Composite Products... Protection Agency has submitted an information collection request (ICR), ``NESHAP for Plywood and Composite... Composite Products covers both new and existing plywood and composite wood products (PCWP) facilities...

  7. 30 CFR 210.204 - How do I submit facility data?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 210.204 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR MINERALS REVENUE MANAGEMENT FORMS AND REPORTS Production and Royalty Reports-Solid Minerals § 210.204 How do I submit facility...) Output quality or product grades. (5) Your submitted facility data may be internally generated documents...

  8. 76 FR 26579 - Procedures for Submitting to the Department of Energy Trade Secrets and Commercial or Financial...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-09

    ... procedures DOE uses to process loan applications submitted to DOE's Advanced Technology Vehicles... information. The procedures are modeled after existing procedures DOE uses to process loan applications... requirements as described above for any information submitted through the Title XVII loan application process...

  9. 76 FR 54243 - Agency Information Collection Activities: Solicitation of Proposal Information for Award of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-31

    ... can propose SBIR research topics and submit proposals in response to SBIR solicitations. In addition... to submit SBIR research topics and submit response to DHS SBIR solicitations. Additionally... 41), under the Small Business Innovative Research (SBIR) and Small Business Technology Transfer (STTR...

  10. 40 CFR 167.90 - Where to obtain and submit forms.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... establishments not found at the same location as their company headquarters, must submit applications and reports... establishments not found at the same location as their company headquarters to be submitted by the company headquarters to the Regional Office having jurisdiction over the State in which the company headquarters is...

  11. 40 CFR 52.69 - Original identification of plan section.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Implementation Plan submitted on March 21, 1972, by the Alabama Air Pollution Control Commission. (2) Compliance schedules submitted on April 18, 1972, by the Alabama Air Pollution Control Commission. (3) Clarifying comments on the plan submitted on April 28, 1972, by the Alabama Air Pollution Control Commission. (4) Semi...

  12. 48 CFR 801.602-84 - Documents to submit for business clearance reviews.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... Development, Contracting Authority, and Responsibilities 801.602-84 Documents to submit for business clearance reviews. A contracting officer must submit to Acquisition Resources Service (Office of Construction and... the vendor is not suspended, debarred, or on the Department of Health and Human Services Exclusionary...

  13. 48 CFR 801.602-84 - Documents to submit for business clearance reviews.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... Development, Contracting Authority, and Responsibilities 801.602-84 Documents to submit for business clearance reviews. A contracting officer must submit to Acquisition Resources Service (Office of Construction and... the vendor is not suspended, debarred, or on the Department of Health and Human Services Exclusionary...

  14. 48 CFR 801.602-84 - Documents to submit for business clearance reviews.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Development, Contracting Authority, and Responsibilities 801.602-84 Documents to submit for business clearance reviews. A contracting officer must submit to Acquisition Resources Service (Office of Construction and... the vendor is not suspended, debarred, or on the Department of Health and Human Services Exclusionary...

  15. 48 CFR 801.602-84 - Documents to submit for business clearance reviews.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... Development, Contracting Authority, and Responsibilities 801.602-84 Documents to submit for business clearance reviews. A contracting officer must submit to Acquisition Resources Service (Office of Construction and... the vendor is not suspended, debarred, or on the Department of Health and Human Services Exclusionary...

  16. 77 FR 70749 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-11-27

    ... released, combusted, or oxidized, industrial gas suppliers, and manufacturers of heavy-duty and off-road... Submitted to OMB for Review and Approval; Comment Request; Greenhouse Gas Reporting Program (Renewal) AGENCY... submitted an information collection request (ICR), Greenhouse Gas Reporting Program (Renewal) (EPA ICR No...

  17. 78 FR 73524 - Information Collection Request Submitted to OMB for Review and Approval; Comment Request; NSPS...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-06

    ... Request Submitted to OMB for Review and Approval; Comment Request; NSPS for Rubber Tire Manufacturing... Protection Agency has submitted an information collection request (ICR), ``NSPS for Rubber Tire Manufacturing.../dockets . Abstract: Respondents are owners or operators of rubber tire manufacturing plants which include...

  18. 40 CFR 52.2475 - Approval of plans.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... submitted by the Washington Department of Ecology on September 20, 2001 and November 22, 2004. (ii) EPA... Plan, adopted April 27, 2004 effective June 24, 2004, submitted by the Washington Department of Ecology... Carbon Monoxide Maintenance Plan submitted by the Washington Department of Ecology on April 25, 2007. (ii...

  19. 40 CFR 52.2475 - Approval of plans.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... submitted by the Washington Department of Ecology on September 20, 2001 and November 22, 2004. (ii) EPA... Plan, adopted April 27, 2004 effective June 24, 2004, submitted by the Washington Department of Ecology... Carbon Monoxide Maintenance Plan submitted by the Washington Department of Ecology on April 25, 2007. (ii...

  20. 40 CFR 52.2475 - Approval of plans.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... submitted by the Washington Department of Ecology on September 20, 2001 and November 22, 2004. (ii) EPA... Plan, adopted April 27, 2004 effective June 24, 2004, submitted by the Washington Department of Ecology... Carbon Monoxide Maintenance Plan submitted by the Washington Department of Ecology on April 25, 2007. (ii...

  1. 7 CFR 3405.12 - Intent to submit a proposal.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 15 2012-01-01 2012-01-01 false Intent to submit a proposal. 3405.12 Section 3405.12 Agriculture Regulations of the Department of Agriculture (Continued) NATIONAL INSTITUTE OF FOOD AND AGRICULTURE HIGHER EDUCATION CHALLENGE GRANTS PROGRAM Submission of a Proposal § 3405.12 Intent to submit a...

  2. 7 CFR 3405.12 - Intent to submit a proposal.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 15 2013-01-01 2013-01-01 false Intent to submit a proposal. 3405.12 Section 3405.12 Agriculture Regulations of the Department of Agriculture (Continued) NATIONAL INSTITUTE OF FOOD AND AGRICULTURE HIGHER EDUCATION CHALLENGE GRANTS PROGRAM Submission of a Proposal § 3405.12 Intent to submit a...

  3. 7 CFR 3405.12 - Intent to submit a proposal.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 15 2014-01-01 2014-01-01 false Intent to submit a proposal. 3405.12 Section 3405.12 Agriculture Regulations of the Department of Agriculture (Continued) NATIONAL INSTITUTE OF FOOD AND AGRICULTURE HIGHER EDUCATION CHALLENGE GRANTS PROGRAM Submission of a Proposal § 3405.12 Intent to submit a...

  4. 7 CFR 3405.12 - Intent to submit a proposal.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false Intent to submit a proposal. 3405.12 Section 3405.12 Agriculture Regulations of the Department of Agriculture (Continued) NATIONAL INSTITUTE OF FOOD AND AGRICULTURE HIGHER EDUCATION CHALLENGE GRANTS PROGRAM Submission of a Proposal § 3405.12 Intent to submit a...

  5. 40 CFR 125.124 - Information required to be submitted by applicant.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... information: (a) An analysis of the chemical constituents of any discharge; (b) Appropriate bioassays... 40 Protection of Environment 23 2013-07-01 2013-07-01 false Information required to be submitted... Ocean Discharge Criteria § 125.124 Information required to be submitted by applicant. The applicant is...

  6. 40 CFR 52.2720 - Identification of plan.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...) Emission limitation for one source in the Ponce Air Basin was submitted on March 26, 1976, by the... schedules submitted on March 13, 1974, by the Puerto Rico Environmental Quality Board. (13) Information on procedures followed in adoption of compliance schedules submitted on March 15, 1974, by the Puerto Rico...

  7. 29 CFR 1952.384 - Completed developmental steps.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 29 CFR 1952.383(g), Puerto Rico submitted its Field Operations Manuals on July 31, 1980, and submitted a revised supplement adopting the Federal OSHA Field Operations Manuals on February 25, 1981. (i... Information System since August of 1978. (j) In accordance with 29 CFR 1952.383(i), Puerto Rico submitted its...

  8. 29 CFR 1952.384 - Completed developmental steps.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 29 CFR 1952.383(g), Puerto Rico submitted its Field Operations Manuals on July 31, 1980, and submitted a revised supplement adopting the Federal OSHA Field Operations Manuals on February 25, 1981. (i... Information System since August of 1978. (j) In accordance with 29 CFR 1952.383(i), Puerto Rico submitted its...

  9. 25 CFR 533.2 - Time for submitting management contracts and amendments.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 2 2010-04-01 2010-04-01 false Time for submitting management contracts and amendments. 533.2 Section 533.2 Indians NATIONAL INDIAN GAMING COMMISSION, DEPARTMENT OF THE INTERIOR MANAGEMENT CONTRACT PROVISIONS APPROVAL OF MANAGEMENT CONTRACTS § 533.2 Time for submitting management contracts and...

  10. 40 CFR 98.5 - How is the report submitted?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 22 2013-07-01 2013-07-01 false How is the report submitted? 98.5 Section 98.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING General Provision § 98.5 How is the report submitted? Each GHG report and...

  11. 40 CFR 98.5 - How is the report submitted?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 21 2011-07-01 2011-07-01 false How is the report submitted? 98.5 Section 98.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING General Provision § 98.5 How is the report submitted? Each GHG report and...

  12. 40 CFR 98.5 - How is the report submitted?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 22 2012-07-01 2012-07-01 false How is the report submitted? 98.5 Section 98.5 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) MANDATORY GREENHOUSE GAS REPORTING General Provision § 98.5 How is the report submitted? Each GHG report and...

  13. 75 FR 5063 - Commission Information Collection Activities (FERC-729); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-02-01

    ... Information Collection Activities (FERC-729); Comment Request; Submitted for OMB Review January 22, 2010... Regulatory Commission (Commission or FERC) has submitted the information collection described below to the Office of Management and Budget (OMB) for review of the information collection requirements. Any...

  14. 76 FR 4888 - Commission Information Collection Activities (FERC-537); Comment Request; Submitted for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-01-27

    ... Information Collection Activities (FERC-537); Comment Request; Submitted for OMB Review January 20, 2011... Regulatory Commission (Commission or FERC) has submitted the information collection described below to the Office of Management and Budget (OMB) for review of the information collection requirements. Any...

  15. 39 CFR 551.4 - Submission requirements and selection criteria.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    .... Interested persons submitting proposals are also encouraged to submit an Adobe Acrobat (.pdf) file saved on a... agencies and causes must satisfy the following requirements: (1) Interested persons must timely submit an original and 20 copies of the proposal. For purposes of this section, interested persons include, but are...

  16. 39 CFR 551.4 - Submission requirements and selection criteria.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    .... Interested persons submitting proposals are also encouraged to submit an Adobe Acrobat (.pdf) file saved on a... agencies and causes must satisfy the following requirements: (1) Interested persons must timely submit an original and 20 copies of the proposal. For purposes of this section, interested persons include, but are...

  17. 39 CFR 551.4 - Submission requirements and selection criteria.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    .... Interested persons submitting proposals are also encouraged to submit an Adobe Acrobat (.pdf) file saved on a... agencies and causes must satisfy the following requirements: (1) Interested persons must timely submit an original and 20 copies of the proposal. For purposes of this section, interested persons include, but are...

  18. 39 CFR 551.4 - Submission requirements and selection criteria.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    .... Interested persons submitting proposals are also encouraged to submit an Adobe Acrobat (.pdf) file saved on a... agencies and causes must satisfy the following requirements: (1) Interested persons must timely submit an original and 20 copies of the proposal. For purposes of this section, interested persons include, but are...

  19. 21 CFR 720.7 - Notification of person submitting cosmetic product ingredient statement.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 7 2012-04-01 2012-04-01 false Notification of person submitting cosmetic product... AND HUMAN SERVICES (CONTINUED) COSMETICS VOLUNTARY FILING OF COSMETIC PRODUCT INGREDIENT COMPOSITION STATEMENTS § 720.7 Notification of person submitting cosmetic product ingredient statement. When Form FDA...

  20. 21 CFR 720.7 - Notification of person submitting cosmetic product ingredient statement.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 7 2011-04-01 2010-04-01 true Notification of person submitting cosmetic product... AND HUMAN SERVICES (CONTINUED) COSMETICS VOLUNTARY FILING OF COSMETIC PRODUCT INGREDIENT COMPOSITION STATEMENTS § 720.7 Notification of person submitting cosmetic product ingredient statement. When Form FDA...

  1. 21 CFR 720.7 - Notification of person submitting cosmetic product ingredient statement.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 7 2014-04-01 2014-04-01 false Notification of person submitting cosmetic product... AND HUMAN SERVICES (CONTINUED) COSMETICS VOLUNTARY FILING OF COSMETIC PRODUCT INGREDIENT COMPOSITION STATEMENTS § 720.7 Notification of person submitting cosmetic product ingredient statement. When Form FDA...

  2. 21 CFR 720.7 - Notification of person submitting cosmetic product ingredient statement.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 7 2010-04-01 2010-04-01 false Notification of person submitting cosmetic product... AND HUMAN SERVICES (CONTINUED) COSMETICS VOLUNTARY FILING OF COSMETIC PRODUCT INGREDIENT COMPOSITION STATEMENTS § 720.7 Notification of person submitting cosmetic product ingredient statement. When Form FDA...

  3. 21 CFR 720.7 - Notification of person submitting cosmetic product ingredient statement.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 7 2013-04-01 2013-04-01 false Notification of person submitting cosmetic product... AND HUMAN SERVICES (CONTINUED) COSMETICS VOLUNTARY FILING OF COSMETIC PRODUCT INGREDIENT COMPOSITION STATEMENTS § 720.7 Notification of person submitting cosmetic product ingredient statement. When Form FDA...

  4. 30 CFR 553.40 - What OSFR evidence must I submit to BOEM?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 30 Mineral Resources 2 2013-07-01 2013-07-01 false What OSFR evidence must I submit to BOEM? 553.40 Section 553.40 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL SPILL FINANCIAL RESPONSIBILITY FOR OFFSHORE FACILITIES Requirements for Submitting OSFR...

  5. 30 CFR 253.40 - What OSFR evidence must I submit to MMS?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 30 Mineral Resources 2 2010-07-01 2010-07-01 false What OSFR evidence must I submit to MMS? 253.40 Section 253.40 Mineral Resources MINERALS MANAGEMENT SERVICE, DEPARTMENT OF THE INTERIOR OFFSHORE OIL SPILL FINANCIAL RESPONSIBILITY FOR OFFSHORE FACILITIES Requirements for Submitting OSFR Information...

  6. 30 CFR 553.40 - What OSFR evidence must I submit to BOEM?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 30 Mineral Resources 2 2012-07-01 2012-07-01 false What OSFR evidence must I submit to BOEM? 553.40 Section 553.40 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL SPILL FINANCIAL RESPONSIBILITY FOR OFFSHORE FACILITIES Requirements for Submitting OSFR...

  7. 30 CFR 553.40 - What OSFR evidence must I submit to BOEM?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 30 Mineral Resources 2 2014-07-01 2014-07-01 false What OSFR evidence must I submit to BOEM? 553.40 Section 553.40 Mineral Resources BUREAU OF OCEAN ENERGY MANAGEMENT, DEPARTMENT OF THE INTERIOR OFFSHORE OIL SPILL FINANCIAL RESPONSIBILITY FOR OFFSHORE FACILITIES Requirements for Submitting OSFR...

  8. 20 CFR 30.701 - How are medical bills to be submitted?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 20 Employees' Benefits 1 2012-04-01 2012-04-01 false How are medical bills to be submitted? 30.701... EMPLOYEES OCCUPATIONAL ILLNESS COMPENSATION PROGRAM ACT OF 2000, AS AMENDED Information for Medical Providers Medical Records and Bills § 30.701 How are medical bills to be submitted? (a) All charges for...

  9. 20 CFR 30.701 - How are medical bills to be submitted?

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 20 Employees' Benefits 1 2014-04-01 2012-04-01 true How are medical bills to be submitted? 30.701... EMPLOYEES OCCUPATIONAL ILLNESS COMPENSATION PROGRAM ACT OF 2000, AS AMENDED Information for Medical Providers Medical Records and Bills § 30.701 How are medical bills to be submitted? (a) All charges for...

  10. 20 CFR 30.701 - How are medical bills to be submitted?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 20 Employees' Benefits 1 2011-04-01 2011-04-01 false How are medical bills to be submitted? 30.701... EMPLOYEES OCCUPATIONAL ILLNESS COMPENSATION PROGRAM ACT OF 2000, AS AMENDED Information for Medical Providers Medical Records and Bills § 30.701 How are medical bills to be submitted? (a) All charges for...

  11. 20 CFR 30.701 - How are medical bills to be submitted?

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 20 Employees' Benefits 1 2013-04-01 2012-04-01 true How are medical bills to be submitted? 30.701... EMPLOYEES OCCUPATIONAL ILLNESS COMPENSATION PROGRAM ACT OF 2000, AS AMENDED Information for Medical Providers Medical Records and Bills § 30.701 How are medical bills to be submitted? (a) All charges for...

  12. Fact Sheet - Managing Ozone Air Quality: Findings on Failure to Submit Elements of 1997 Ozone NAAQS State Implementation Plan

    EPA Pesticide Factsheets

    This fact sheet provides a basic overview of the May 17, 2008 notice that 11 states missed their deadlines to submit their state implementation plans (SIPs). The deadlines are for submitting complete plans showing how they will meet the 1997 ozone standard

  13. 78 FR 72139 - Forms Submitted to the Office of Management and Budget for Extension of Clearance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-02

    ... should be sent to the Office of Information and Regulatory Affairs, Attention: Desk Officer, Selective... SELECTIVE SERVICE SYSTEM Forms Submitted to the Office of Management and Budget for Extension of Clearance AGENCY: Selective Service System. ACTION: Notice. The following forms have been submitted to the...

  14. 76 FR 70803 - Form Submitted to the Office of Management and Budget for Extension of Clearance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-15

    ... sent to the Office of Information and Regulatory Affairs, Attention: Desk Officer, Selective Service... SELECTIVE SERVICE SYSTEM Form Submitted to the Office of Management and Budget for Extension of Clearance AGENCY: Selective Service System. ACTION: Notice. The following forms have been submitted to the...

  15. 76 FR 62892 - Form Submitted to the Office of Management and Budget for Extension of Clearance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-11

    ... should be sent to the Office of Information and Regulatory Affairs, Attention: Desk Officer, Selective... SELECTIVE SERVICE SYSTEM Form Submitted to the Office of Management and Budget for Extension of Clearance AGENCY: Selective Service System. ACTION: Notice. The following form has been submitted to the...

  16. 76 FR 12394 - Form Submitted to the Office of Management and Budget for Extension of Clearance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-07

    ... SELECTIVE SERVICE SYSTEM Form Submitted to the Office of Management and Budget for Extension of Clearance AGENCY: Selective Service System. ACTION: Notice. The following form has been submitted to the... boards in the Selective Service System. Respondents: Potential board members. Burden: A burden of 15...

  17. 75 FR 53012 - Form Submitted to the Office of Management and Budget for Extension of Clearance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-30

    ... Regulatory Affairs, Attention: Desk Officer, Selective Service System, Office of Management and Budget, New... SELECTIVE SERVICE SYSTEM Form Submitted to the Office of Management and Budget for Extension of Clearance AGENCY: Selective Service System. ACTION: Notice. The following form has been submitted to the...

  18. 76 FR 41855 - Forms Submitted to the Office of Management and Budget for Extension of Clearance

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-15

    ... Regulatory Affairs, Attention: Desk Officer, Selective Service System, Office of Management and Budget, New... SELECTIVE SERVICE SYSTEM Forms Submitted to the Office of Management and Budget for Extension of Clearance AGENCY: Selective Service System. ACTION: Notice. The following form has been submitted to the...

  19. 46 CFR 110.25-3 - Procedure for submitting plans.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Procedure for submitting plans. 110.25-3 Section 110.25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING GENERAL PROVISIONS Plan Submittal § 110.25-3 Procedure for submitting plans. (a) The plans required by § 110.25-1...

  20. 46 CFR 110.25-3 - Procedure for submitting plans.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Procedure for submitting plans. 110.25-3 Section 110.25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING GENERAL PROVISIONS Plan Submittal § 110.25-3 Procedure for submitting plans. (a) The plans required by § 110.25-1...

  1. 46 CFR 110.25-3 - Procedure for submitting plans.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Procedure for submitting plans. 110.25-3 Section 110.25-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) ELECTRICAL ENGINEERING GENERAL PROVISIONS Plan Submittal § 110.25-3 Procedure for submitting plans. (a) The plans required by § 110.25-1...

  2. 78 FR 77479 - Agency Information Collection Activities: Proposed Collection; Comment Request; Revision to...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-23

    ... and Docket ID. Regardless of the method used for submitting comments or material, all submissions will... flood hazards when physical changes occur. In 44 CFR 65.4, communities are provided the right to submit... the NFIP maps, a community must submit scientific or technical data demonstrating the need for a...

  3. 49 CFR 382.211 - Refusal to submit to a required alcohol or controlled substances test.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ....211 Refusal to submit to a required alcohol or controlled substances test. No driver shall refuse to submit to a pre-employment controlled substance test required under § 382.301, a post-accident alcohol or... controlled substances test. 382.211 Section 382.211 Transportation Other Regulations Relating to...

  4. 49 CFR 382.211 - Refusal to submit to a required alcohol or controlled substances test.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ....211 Refusal to submit to a required alcohol or controlled substances test. No driver shall refuse to submit to a pre-employment controlled substance test required under § 382.301, a post-accident alcohol or... controlled substances test. 382.211 Section 382.211 Transportation Other Regulations Relating to...

  5. 49 CFR 382.211 - Refusal to submit to a required alcohol or controlled substances test.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ....211 Refusal to submit to a required alcohol or controlled substances test. No driver shall refuse to submit to a post-accident alcohol or controlled substances test required under § 382.303, a random... controlled substances test. 382.211 Section 382.211 Transportation Other Regulations Relating to...

  6. 49 CFR 382.211 - Refusal to submit to a required alcohol or controlled substances test.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ....211 Refusal to submit to a required alcohol or controlled substances test. No driver shall refuse to submit to a post-accident alcohol or controlled substances test required under § 382.303, a random... controlled substances test. 382.211 Section 382.211 Transportation Other Regulations Relating to...

  7. 49 CFR 382.211 - Refusal to submit to a required alcohol or controlled substances test.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ....211 Refusal to submit to a required alcohol or controlled substances test. No driver shall refuse to submit to a pre-employment controlled substance test required under § 382.301, a post-accident alcohol or... controlled substances test. 382.211 Section 382.211 Transportation Other Regulations Relating to...

  8. 75 FR 3121 - Delegations of Authority to the Director of Its Division of Enforcement

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-01-19

    ... Enforcement (``Division'') to submit witness immunity order requests to the Department of Justice for... Director of the Division of Enforcement (``Director'') to submit witness immunity order requests to the...) To submit witness immunity requests to the U.S. Attorney General for approval to seek an order...

  9. 78 FR 18575 - Agency Information Collection Activities; Submission to the Office of Management and Budget for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-03-27

    ... hand delivery. Please note that comments submitted by fax or email and those submitted after the comment period will not be accepted. Written requests for information or comments submitted by postal mail..., including through the use of information technology. Please note that written comments received in response...

  10. 78 FR 6082 - Agency Information Collection Activities; Submission to the Office of Management and Budget for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-29

    ... hand delivery. Please note that comments submitted by fax or email and those submitted after the comment period will not be accepted. Written requests for information or comments submitted by postal mail..., including through the use of information technology. Please note that written comments received in response...

  11. 78 FR 6082 - Agency Information Collection Activities; Submission to the Office of Management and Budget for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-29

    ... delivery, or hand delivery. Please note that comments submitted by fax or email and those submitted after the comment period will not be accepted. Written requests for information or comments submitted by... respondents, including through the use of information technology. Please note that written comments received...

  12. 78 FR 21601 - Agency Information Collection Activities; Submission to the Office of Management and Budget for...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-11

    ... delivery, or hand delivery. Please note that comments submitted by fax or email and those submitted after the comment period will not be accepted. Written requests for information or comments submitted by... respondents, including through the use of information technology. Please note that written comments received...

  13. 78 FR 42085 - Draft Guidance for Industry on Pediatric Study Plans: Content of and Process for Submitting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-15

    ...] Draft Guidance for Industry on Pediatric Study Plans: Content of and Process for Submitting Initial... a draft guidance for industry entitled ``Pediatric Study Plans: Content of and Process for... Plans: Content of and Process for Submitting Initial Pediatric Study Plans and Amended Pediatric Study...

  14. 21 CFR 1.280 - How must you submit prior notice?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... to FDA. You must submit all prior notice information in the English language, except that an... Commercial System (ABI/ACS); or (2) The FDA PNSI at http://www.access.fda.gov. You must submit prior notice through the FDA Prior Notice System Interface (FDA PNSI) for articles of food imported or offered for...

  15. 30 CFR 210.101 - Who must submit production reports?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MANAGEMENT FORMS AND REPORTS Production Reports-Oil and Gas § 210.101 Who must submit production reports? (a... operator number. To obtain an operator number, refer to the MMS Minerals Production Reporter Handbook for... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Who must submit production reports? 210.101...

  16. 30 CFR 210.104 - Must I submit these production reports electronically?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... MINERALS REVENUE MANAGEMENT FORMS AND REPORTS Production Reports-Oil and Gas § 210.104 Must I submit these... Production Reporter Handbook for the most current reporting options, instructions, and security measures. The... 30 Mineral Resources 2 2010-07-01 2010-07-01 false Must I submit these production reports...

  17. 27 CFR 19.112 - General rules for amending a registration.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... specified in this subpart. To amend a registration the proprietor must submit in writing to the appropriate..., require that the proprietor submit an amended application on TTB F 5110.41 if administrative difficulties... proprietor submit an amended application on TTB F 5110.41 if administrative difficulties occur as a result of...

  18. 27 CFR 19.112 - General rules for amending a registration.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... specified in this subpart. To amend a registration the proprietor must submit in writing to the appropriate..., require that the proprietor submit an amended application on TTB F 5110.41 if administrative difficulties... proprietor submit an amended application on TTB F 5110.41 if administrative difficulties occur as a result of...

  19. 27 CFR 19.112 - General rules for amending a registration.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... specified in this subpart. To amend a registration the proprietor must submit in writing to the appropriate..., require that the proprietor submit an amended application on TTB F 5110.41 if administrative difficulties... proprietor submit an amended application on TTB F 5110.41 if administrative difficulties occur as a result of...

  20. 27 CFR 19.112 - General rules for amending a registration.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... specified in this subpart. To amend a registration the proprietor must submit in writing to the appropriate..., require that the proprietor submit an amended application on TTB F 5110.41 if administrative difficulties... proprietor submit an amended application on TTB F 5110.41 if administrative difficulties occur as a result of...

  1. Content Analysis of Papers Submitted to "Communications in Information Literacy," 2007-2013

    ERIC Educational Resources Information Center

    Hollister, Christopher V.

    2014-01-01

    The author conducted a content analysis of papers submitted to the journal, "Communications in Information Literacy," from the years 2007-2013. The purpose was to investigate and report on the overall quality characteristics of a statistically significant sample of papers submitted to a single-topic, open access, library and information…

  2. 40 CFR 152.93 - Citation of a previously submitted valid study.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Data Submitters' Rights § 152.93 Citation of a previously submitted valid study. An applicant may demonstrate compliance for a data requirement by citing a valid study previously submitted to the Agency. The... the original data submitter, the applicant may cite the study only in accordance with paragraphs (b...

  3. 17 CFR 240.14a-18 - Disclosure regarding nominating shareholders and nominees submitted for inclusion in a registrant...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... nominating shareholders and nominees submitted for inclusion in a registrant's proxy materials pursuant to... nominees submitted for inclusion in a registrant's proxy materials pursuant to applicable state or foreign... governing documents addressing the inclusion of shareholder director nominees in the registrant's proxy...

  4. 17 CFR 240.14a-18 - Disclosure regarding nominating shareholders and nominees submitted for inclusion in a registrant...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... nominating shareholders and nominees submitted for inclusion in a registrant's proxy materials pursuant to... nominees submitted for inclusion in a registrant's proxy materials pursuant to applicable state or foreign... governing documents addressing the inclusion of shareholder director nominees in the registrant's proxy...

  5. 17 CFR 240.14a-18 - Disclosure regarding nominating shareholders and nominees submitted for inclusion in a registrant...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... nominating shareholders and nominees submitted for inclusion in a registrant's proxy materials pursuant to... nominees submitted for inclusion in a registrant's proxy materials pursuant to applicable state or foreign... governing documents addressing the inclusion of shareholder director nominees in the registrant's proxy...

  6. 20 CFR 30.518 - Can OWCP require the recipient of the overpayment to submit additional financial information?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... overpayment to submit additional financial information? 30.518 Section 30.518 Employees' Benefits OFFICE OF... OWCP require the recipient of the overpayment to submit additional financial information? (a) The... specified by OWCP. This information is needed to determine whether or not recovery of an overpayment would...

  7. 20 CFR 30.518 - Can OWCP require the recipient of the overpayment to submit additional financial information?

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... overpayment to submit additional financial information? 30.518 Section 30.518 Employees' Benefits OFFICE OF... OWCP require the recipient of the overpayment to submit additional financial information? (a) The... specified by OWCP. This information is needed to determine whether or not recovery of an overpayment would...

  8. 20 CFR 30.518 - Can OWCP require the recipient of the overpayment to submit additional financial information?

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... overpayment to submit additional financial information? 30.518 Section 30.518 Employees' Benefits OFFICE OF... OWCP require the recipient of the overpayment to submit additional financial information? (a) The... specified by OWCP. This information is needed to determine whether or not recovery of an overpayment would...

  9. 43 CFR 3278.10 - When will BLM disclose information I submit under these regulations?

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... covering public disclosure of data and information contained in Department records. Certain mineral information not protected from disclosure under part 2 may be made available for inspection without a Freedom... information I submit under these regulations? All Federal and Indian data and information submitted to BLM are...

  10. 43 CFR 3278.10 - When will BLM disclose information I submit under these regulations?

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... covering public disclosure of data and information contained in Department records. Certain mineral information not protected from disclosure under part 2 may be made available for inspection without a Freedom... information I submit under these regulations? All Federal and Indian data and information submitted to BLM are...

  11. 77 FR 36012 - PPL Bell Bend, LLC; Bell Bend Nuclear Power Plant Combined License Application; Notice of Intent...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-06-15

    .... DATES: Please submit any comments by July 16, 2012. ADDRESSES: You may access information and comment... information and submitting comments, see ``Accessing Information and Submitting Comments'' in the SUPPLEMENTARY INFORMATION section of this document. Email Comments to: [email protected] . To ensure that...

  12. 40 CFR 60.2755 - When must I submit my waste management plan?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 6 2010-07-01 2010-07-01 false When must I submit my waste management... waste management plan? You must submit the waste management plan no later than the date specified in... Compliance Times for Commercial and Industrial Solid Waste Incineration Units that Commenced Construction On...

  13. 40 CFR 55.4 - Requirements to submit a notice of intent.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... intent. 55.4 Section 55.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) OUTER CONTINENTAL SHELF AIR REGULATIONS § 55.4 Requirements to submit a notice of... Regional Office, and at the same time shall submit copies of the NOI to the air pollution control agencies...

  14. 40 CFR 55.4 - Requirements to submit a notice of intent.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... intent. 55.4 Section 55.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) OUTER CONTINENTAL SHELF AIR REGULATIONS § 55.4 Requirements to submit a notice of... Regional Office, and at the same time shall submit copies of the NOI to the air pollution control agencies...

  15. 40 CFR 55.4 - Requirements to submit a notice of intent.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... intent. 55.4 Section 55.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) OUTER CONTINENTAL SHELF AIR REGULATIONS § 55.4 Requirements to submit a notice of... Regional Office, and at the same time shall submit copies of the NOI to the air pollution control agencies...

  16. 40 CFR 55.4 - Requirements to submit a notice of intent.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... intent. 55.4 Section 55.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) OUTER CONTINENTAL SHELF AIR REGULATIONS § 55.4 Requirements to submit a notice of... Regional Office, and at the same time shall submit copies of the NOI to the air pollution control agencies...

  17. 40 CFR 55.4 - Requirements to submit a notice of intent.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... intent. 55.4 Section 55.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR PROGRAMS (CONTINUED) OUTER CONTINENTAL SHELF AIR REGULATIONS § 55.4 Requirements to submit a notice of... Regional Office, and at the same time shall submit copies of the NOI to the air pollution control agencies...

  18. 25 CFR 20.601 - How can applications be submitted?

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false How can applications be submitted? 20.601 Section 20.601... SOCIAL SERVICES PROGRAMS Administrative Procedures § 20.601 How can applications be submitted? You can... you can get from your social services worker or tribe; or (b) Through an interview with a social...

  19. 44 CFR 204.22 - Submitting a request for a fire management assistance declaration.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... ASSISTANCE GRANT PROGRAM Declaration Process § 204.22 Submitting a request for a fire management assistance.... To ensure that we can process a State's request for a fire management assistance declaration as... 44 Emergency Management and Assistance 1 2014-10-01 2014-10-01 false Submitting a request for a...

  20. 44 CFR 204.22 - Submitting a request for a fire management assistance declaration.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... ASSISTANCE GRANT PROGRAM Declaration Process § 204.22 Submitting a request for a fire management assistance.... To ensure that we can process a State's request for a fire management assistance declaration as... 44 Emergency Management and Assistance 1 2011-10-01 2011-10-01 false Submitting a request for a...

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