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Sample records for library immunization confers

  1. Expression Library Immunization Can Confer Protection against Lethal Challenge with African Swine Fever Virus

    PubMed Central

    Lacasta, Anna; Ballester, María; Monteagudo, Paula L.; Rodríguez, Javier M.; Salas, María L.; Accensi, Francesc; Pina-Pedrero, Sonia; Bensaid, Albert; Argilaguet, Jordi; López-Soria, Sergio; Hutet, Evelyne; Le Potier, Marie Frédérique

    2014-01-01

    ABSTRACT African swine fever is one of the most devastating pig diseases, against which there is no vaccine available. Recent work from our laboratory has demonstrated the protective potential of DNA vaccines encoding three African swine fever viral antigens (p54, p30, and the hemagglutinin extracellular domain) fused to ubiquitin. Partial protection was afforded in the absence of detectable antibodies prior to virus challenge, and survival correlated with the presence of a large number of hemagglutinin-specific CD8+ T cells in blood. Aiming to demonstrate the presence of additional CD8+ T-cell determinants with protective potential, an expression library containing more than 4,000 individual plasmid clones was constructed, each one randomly containing a Sau3AI restriction fragment of the viral genome (p54, p30, and hemagglutinin open reading frames [ORFs] excluded) fused to ubiquitin. Immunization of farm pigs with the expression library yielded 60% protection against lethal challenge with the virulent E75 strain. These results were further confirmed by using specific-pathogen-free pigs after challenging them with 104 hemadsorbing units (HAU) of the cell culture-adapted strain E75CV1. On this occasion, 50% of the vaccinated pigs survived the lethal challenge, and 2 out of the 8 immunized pigs showed no viremia or viral excretion at any time postinfection. In all cases, protection was afforded in the absence of detectable specific antibodies prior to challenge and correlated with the detection of specific T-cell responses at the time of sacrifice. In summary, our results clearly demonstrate the presence of additional protective determinants within the African swine fever virus (ASFV) genome and open up the possibility for their future identification. IMPORTANCE African swine fever is a highly contagious disease of domestic and wild pigs that is endemic in many sub-Saharan countries, where it causes important economic losses and is currently in continuous expansion

  2. Expression library immunization confers protection against Mycobacterium avium subsp. paratuberculosis infection.

    PubMed

    Huntley, J F; Stabel, J R; Paustian, M L; Reinhardt, T A; Bannantine, J P

    2005-10-01

    Currently, paratuberculosis vaccines are comprised of crude whole-cell preparations of Mycobacterium avium subsp. paratuberculosis. Although effective in reducing clinical disease and fecal shedding, these vaccines have severe disadvantages as well, including seroconversion of vaccinated animals and granulomatous lesions at the site of vaccination. DNA vaccines can offer an alternative approach that may be safer and elicit more protective responses. In an effort to identify protective M. avium subsp. paratuberculosis sequences, a genomic DNA expression library was generated and subdivided into pools of clones (approximately 1,500 clones/pool). The clone pools were evaluated to determine DNA vaccine efficacy by immunizing mice via gene gun delivery and challenging them with live, virulent M. avium subsp. paratuberculosis. Four clone pools resulted in a significant reduction in the amount of M. avium subsp. paratuberculosis recovered from mouse tissues compared to mice immunized with other clone pools and nonvaccinated, infected control mice. One of the protective clone pools was further partitioned into 10 clone arrays of 108 clones each, and four clone arrays provided significant protection from both spleen and mesenteric lymph node colonization by M. avium subsp. paratuberculosis. The nucleotide sequence of each clone present in the protective pools was determined, and coding region functions were predicted by computer analysis. Comparison of the protective clone array sequences implicated 26 antigens that may be responsible for protection in mice. This study is the first study to demonstrate protection against M. avium subsp. paratuberculosis infection with expression library immunization. PMID:16177367

  3. IEEE Conference Publications in Libraries.

    ERIC Educational Resources Information Center

    Johnson, Karl E.

    1984-01-01

    Conclusions of surveys (63 libraries, OCLC database, University of Rhode Island users) assessing handling of Institute of Electrical and Electronics Engineers (IEEE) conference publications indicate that most libraries fully catalog these publications using LC cataloging, and library patrons frequently require series access to publications. Eight…

  4. Reviewing the Electronic Resources & Libraries Conference

    ERIC Educational Resources Information Center

    Tijerina, Bonnie

    2008-01-01

    The third Electronic Resources & Libraries (ER&L) conference gathered at Georgia Institute of Technology's Global Learning and Conference Center in Atlanta, Georgia, March 18-21, 2008. Over 360 attendees, from six countries and from 80% of the United States, represented their libraries and organizations resulting in a diverse and informative…

  5. IFLA General Conference, 1986. Libraries Serving the General Public Division. Section: School Libraries. Childrens' Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on school libraries which were presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "School Libraries: Bridges or Barriers?" (Gwen Gawith, New Zealand); (2) "The Guidance on Reading and Information Skills in School Libraries--Japan's Case" (Ikuko Koyama, Japan); (3) "Present Status and…

  6. Ohio White House Conference on Library and Information Services: Speak Out. Conference Background Information.

    ERIC Educational Resources Information Center

    Ohio State Library, Columbus.

    Developed as background material for the 1978 Ohio White House Conference on Library and Information Services, this document provides information in six areas of concern for Ohio libraries: (1) library and information services--library users, library collections, special user needs; (2) public awareness--definition, current status, suggestions for…

  7. IFLA General Conference, 1986. General Research Libraries Division. Section: Parliamentary Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on parliamentary libraries presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Library Support for Parliamentary Committees" (Peter Dawe, Switzerland), which considers the needs of parliamentary committees in terms of library materials and services; (2) "The National Assembly Library (NAL)…

  8. Implications of the White House Conference on Library and Information Services for Library Education.

    ERIC Educational Resources Information Center

    Journal of Education for Librarianship, 1981

    1981-01-01

    Assesses the significance of the Conference on five areas toward which library educators can focus attention: (1) public awareness of libraries, (2) user-oriented library services, (3) resource sharing and networking, (4) enabling mechanisms for library legislation and funding, and (5) library research needs. Fifteen references are listed. (FM)

  9. IFLA General Conference, 1984. Special Libraries Division. Section on Science and Technology Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on scientific/technical information and libraries presented at the 1984 IFLA general conference include: (1) "Library Ethics and the Special Library Network in Science and Technology" (Dieter Schmidmaier, East Germany); (2) "The Dissemination of Patent Information by Libraries: An Example Demonstrating the Necessity of Libraries in the…

  10. Hypothesis: how licensed vaccines confer protective immunity.

    PubMed

    Robbins, J B; Schneerson, R; Szu, S C

    1996-01-01

    By examining experience with evaluation of licensed vaccines we theorize that a critical level of serum IgG confers protection against infectious diseases by killing or inactivating the inoculum. We found that efficacy is reliably predicted by measurement of serum antibodies elicited by vaccines, that serum IgG antibodies alone account for the protection conferred by passive immunization, that vaccine-induced "herd" immunity is best explained by inactivation of the inoculum on epithelial surfaces by serum antibodies and that serum antibodies induced by active immunization will neither treat disease symptoms nor eliminate the pathogen. If valid, this theory should facilitate research because knowledge of the pathogenesis of the disease symptoms may not be essential for vaccine development.

  11. IFLA General Conference, 1986. Special Libraries Division. Section: Social Science Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on social science libraries presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Efforts at Computerization in Nigerian Libraries--A State of Development Review" (A. Olugboyega Banjo, Nigeria); (2) "The Information Activities of the National Library of Economics in the Federal Republic of…

  12. IFLA General Conference, 1986. Libraries Serving the General Public Division. Section: Public Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on public libraries presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Developing Children's Libraries in Shanghai" (Che Jiuzhi, China); (2) "The Development of Public Librarianship in China" (Cheng Fuchen, China); (3) "The Development of Public Libraries in Japan" (Mamoru Nogami, Japan);…

  13. IFLA General Conference, 1986. Special Libraries Division. Section: Administrative Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Four papers on administrative libraries were presented at the 1986 International Federation of Library Associations (IFLA) conference. "Special Libraries and Their Activities in Japan" (Masaya Takayama, Japan) discusses statistical investigation into the conditions of Japanese special libraries, information needs and uses, the present situation…

  14. IFLA General Conference, 1985. Division on Libraries Serving the General Public. Section on Public Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    This document includes papers on public libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference. The first paper, "International Guidelines for Public Libraries," is the report of an IFLA working group and it includes guidelines in six areas: public library services; media of communication;…

  15. IFLA General Conference, 1985. Division of General Research Libraries. Section on National Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on national libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The Lenin State Library in Transition" (N. S. Kartashov, The Lenin State Library, USSR); (2) "Preserving for Access" (Guy Sylvestre, Canadian Institute for Historical Microreproductions, Canada); (3) "The…

  16. IFLA General Conference, 1986. Libraries Serving the General Public Division. Section: Libraries for Disadvantaged People. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on libraries for disadvantaged people presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "The Hearing Impaired Persons and Libraries--The Status Quo in Japan" (Shuuichi Matsunobu, Japan); (2) "A Production System of Captioned Video Tapes Using Japanese Word Processors" (Satoshi Nishikawa,…

  17. IFLA General Conference, 1986. General Research Libraries Division. Section: National Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on national libraries which were presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Dangers for the Modern Information Society in the Computer Age" (Franz Georg Kaltwasser, West Germany); (2) "Functions and Objectives of the National Libraries in the Developing Countries" (J. Le Riverend,…

  18. IFLA General Conference, 1985. Division on Libraries Serving the General Public. Section on School Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    This document contains two papers on school libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference. In "Teachers, Information and School Libraries," Noelene Hall (Australia) discusses the activities of an international working group set up by IFLA in 1984 to develop guidelines for the…

  19. IFLA General Conference, 1985. Division on Special Libraries. Section on Administrative Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on administrative libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Administrative Libraries and Universal Availability of Information: Comments from a Developing Country" (Diekolola Awosika, Nigeria); (2) "Automatic Indexing and the Dissemination of Information"…

  20. IFLA General Conference, 1985. Division of General Research Libraries. Section on Parliamentary Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on parliamentary libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Cooperation among Parliamentary Libraries--Example: Federal Republic of Germany" (Wolfgang Dietz, West Germany); (2) "Cooperative Development in Action: Towards an Australian Parliamentary Information…

  1. IFLA General Conference, 1985. Division on Special Libraries. Section on Biological and Medical Science Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on biological and medical science libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The International Programs of the National Library of Medicine" (Lois Ann Colaianni, United States); (2) "Information Needs for International Health. A CDC (Centers for Disease…

  2. IFLA General Conference, 1984. General Research Libraries Division. Section on Parliamentary Libraries; Section on Public Libraries; Section on University and Other General Research Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on government libraries, public libraries, and research libraries presented at the 1984 IFLA general conference include: (1) "Library Services for Research" (Maria S. Pla de Menendez, Colombia); (2) "Interlibrary Loans, Present and Future: A Consideration for Academic Library Management" (Geoffrey G. Allen, Australia); (3) "The…

  3. International Federation of Library Associations Annual Conference Papers. General Research Libraries Division: University Libraries Section (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Loveday, Anthony J.; And Others

    This set of papers presented to the General Research Libraries Division, University Libraries Section, of the International Federation of Library Associations during its 47th annual conference (1981) includes: "SCONUL (Standing Conference of National and University Libraries) and British University Library Standards: Some Observations on the Role…

  4. International Federation of Library Associations Annual Conference Papers. General Research Libraries Division: Parliamentary Libraries and National Libraries Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Gude, Gilbert; And Others

    This set of papers presented to the General Research Libraries Division of the International Federation of Library Associations (IFLA) during its 47th annual conference (1981) includes: "The Effect of the Introduction of Computers on Library and Research Staff," by Gilbert Gude; "Libraries as Information Service Agencies (IVS)," by Franz Georg…

  5. Protective antigens against glanders identified by expression library immunization.

    PubMed

    Whitlock, Gregory C; Robida, Mark D; Judy, Barbara M; Qazi, Omar; Brown, Katherine A; Deeraksa, Arpaporn; Taylor, Katherine; Massey, Shane; Loskutov, Andrey; Borovkov, Alex Y; Brown, Kevin; Cano, Jose A; Torres, Alfredo G; Estes, D Mark; Sykes, Kathryn F

    2011-01-01

    Burkholderia are highly evolved Gram-negative bacteria that primarily infect solipeds but are transmitted to humans by ingestion and cutaneous or aerosol exposures. Heightened concern over human infections of Burkholderia mallei and the very closely related species B. pseudomallei is due to the pathogens' proven effectiveness as bioweapons, and to the increased potential for natural opportunistic infections in the growing diabetic and immuno-compromised populations. These Burkholderia species are nearly impervious to antibiotic treatments and no vaccine exists. In this study, the genome of the highly virulent B. mallei ATCC23344 strain was examined by expression library immunization for gene-encoded protective antigens. This protocol for genomic-scale functional screening was customized to accommodate the unusually large complexity of Burkholderia, and yielded 12 new putative vaccine candidates. Five of the candidates were individually tested as protein immunogens and three were found to confer significant partial protection against a lethal pulmonary infection in a murine model of disease. Determinations of peripheral blood cytokine and chemokine profiles following individual protein immunizations show that interleukin-2 (IL-2) and IL-4 are elicited by the three confirmed candidates, but unexpectedly interferon-γ and tumor necrosis factor-α are not. We suggest that these pathogen components, discovered using genetic immunization and confirmed in a conventional protein format, will be useful toward the development of a safe and effective glanders vaccine.

  6. Protective Antigens Against Glanders Identified by Expression Library Immunization

    PubMed Central

    Whitlock, Gregory C.; Robida, Mark D.; Judy, Barbara M.; Qazi, Omar; Brown, Katherine A.; Deeraksa, Arpaporn; Taylor, Katherine; Massey, Shane; Loskutov, Andrey; Borovkov, Alex Y.; Brown, Kevin; Cano, Jose A.; Magee, D. Mitchell; Torres, Alfredo G.; Estes, D. Mark; Sykes, Kathryn F.

    2011-01-01

    Burkholderia are highly evolved Gram-negative bacteria that primarily infect solipeds but are transmitted to humans by ingestion and cutaneous or aerosol exposures. Heightened concern over human infections of Burkholderia mallei and the very closely related species B. pseudomallei is due to the pathogens’ proven effectiveness as bioweapons, and to the increased potential for natural opportunistic infections in the growing diabetic and immuno-compromised populations. These Burkholderia species are nearly impervious to antibiotic treatments and no vaccine exists. In this study, the genome of the highly virulent B. mallei ATCC23344 strain was examined by expression library immunization for gene-encoded protective antigens. This protocol for genomic-scale functional screening was customized to accommodate the unusually large complexity of Burkholderia, and yielded 12 new putative vaccine candidates. Five of the candidates were individually tested as protein immunogens and three were found to confer significant partial protection against a lethal pulmonary infection in a murine model of disease. Determinations of peripheral blood cytokine and chemokine profiles following individual protein immunizations show that interleukin-2 (IL-2) and IL-4 are elicited by the three confirmed candidates, but unexpectedly interferon-γ and tumor necrosis factor-α are not. We suggest that these pathogen components, discovered using genetic immunization and confirmed in a conventional protein format, will be useful toward the development of a safe and effective glanders vaccine. PMID:22125550

  7. IFLA General Conference, 1984. Special Libraries Division. Section on Social Science Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The two papers in this document on social science libraries were presented at the 1984 IFLA general conference. In "Library and Continuing Education with Implications for Developing Countries: A Research Essay," David R. Bender (United States) examines factors impacting upon the skills necessary for effective librarianship in the social sciences,…

  8. IFLA General Conference, 1986. Special Libraries Division. Section: Art Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on art libraries presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "The Change of Illustrated Story Books in the Edo Period (1660-1880)" (Yaeko Kimura, Japan); (2) "History of Posters in Japan and the Present States of Their Documentation" (Itsuo Okubo, Japan); (3) "Solutions to Processing…

  9. IFLA General Conference, 1986. Special Libraries Division. Section: Geography and Map Library. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Four papers on geography and map libraries were presented at the 1986 International Federation of Library Associations (IFLA) conference. "Generation and Utilization of Maps and Atlases in Japan," by Takashi Morita of Japan, presents an overview of the making and uses of maps and atlases in Japan and concludes that a comprehensive national map…

  10. IFLA General Conference, 1985. Division on Special Libraries. Section on Science and Technology Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on science and technology libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "UAP (Universal Availability of Publications) and User Training for Categories of Grey Literature" (Dieter Schmidmaier, Mining Academy Freiberg, East Germany); (2) "Resource Sharing in Science…

  11. IFLA General Conference, 1985. Division on Special Libraries. Section on Art Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on art libraries and art documentation which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The Universal Availability of Art Publications: A Global Context" (Philip Pacey, United Kingdom); (2) "Art Librarianship in Norway" (Anne Lise Rabben and Cecilie W. Bonafede, Norway); (3)…

  12. IFLA General Conference, 1985. Division on Special Libraries. Section on Social Science Libraries and Geography and Map Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers presented on social science and map and geography libraries at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Information for the Developing World: NTIS's (National Technical Information Service) Role in Information Transfer to Developing Countries" (Joseph F. Caponio, United States); (2)…

  13. IFLA General Conference, 1986. Education and Research Division. Section: Library History and Library Theory and Research. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on library history and library theory and research presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "The History of Western Literature Library Collections in Japan" (Yoshitomi Okazaki, Japan); (2) "Trends of Library History Research in Japan" (Hiroshi Kawai, Yukio Fujino, Atsushi Ishii,…

  14. National Conference on Library Statistics (Chicago, June 6-8, 1966).

    ERIC Educational Resources Information Center

    Trezza, Alphonse F., Ed.; Beasley, James, Ed.

    Attended by representatives of American Library Association (ALA) divisions and committees, other national library associations, state library agencies, library schools, and all types of libraries, this conference was held to discuss: (1) the program of the National Center for Educational Statistics (USOE) as it pertains to libraries; (2) the…

  15. IFLA General Conference, 1985. Division on Libraries Serving the General Public. Section on Libraries Serving Disadvantaged People. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    This document contains two papers on library services in prisons which were presented at the 1985 International Federation of Library Associations (IFLA) conference. In the paper, "Library Service in Prisons/Correctional Institutions," Phyllis I. Dalton (United States) proposes that the IFLA Section on Libraries Serving Disadvantaged Persons…

  16. IFLA General Conference, 1985. Division of Libraries Serving the General Public. Section on Libraries for the Blind. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library services and programs for the blind and visually handicapped which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Copyright and Library Material for the Blind" (Elizabeth Twining, United Kingdom); (2) "Postal and Customs Regulations and Library Material for the Blind"…

  17. Brick & Click Libraries: An Academic Library Conference (15th, Maryville, Missouri, November 6, 2015)

    ERIC Educational Resources Information Center

    Baudino, Frank, Ed.; Johnson, Carolyn, Ed.

    2015-01-01

    Nineteen scholarly papers and seventeen abstracts comprise the content of the fifteenth annual Brick and Click Libraries Conference, held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship.…

  18. IFLA General Conference, 1984. Special Libraries Division. Section on Geography and Map Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    This document comprises two papers on geography and map libraries and cartographic materials which were presented at the 1984 IFLA general conference. "Cartographic Materials as a Resource for National and Regional Planning and Development," by E. Hans van de Waal (Utrecht), addresses the importance of cartographic materials for development…

  19. The White House Conference on School Libraries Proceedings (Washington, DC, June 4, 2002).

    ERIC Educational Resources Information Center

    Institute of Museum and Library Services, Washington, DC.

    Laura Bush hosted a White House Conference on School Libraries on June 4, 2002, to discuss the latest research on libraries, student achievement and successful local programs. Mrs. Bush's cohost was Dr. Robert Martin, Director of the Institute of Museum and Library Services and education, library, government and philanthropic leaders from across…

  20. IFLA General Conference, 1986. Management and Technology Division. Section: Library Buildings and Equipment. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on library buildings and equipment which were presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Changes in Library Buildings" (H. Faulkner-Brown, United Kingdom), which defines a desirable library building as being flexible, compact, accessible, extendable, varied, organized, comfortable,…

  1. New Jersey Governor's Conference on Library and Information Services. Delegate Resolutions.

    ERIC Educational Resources Information Center

    1979

    Written and adopted by delegates to the Governor's Conference in Spring 1979, this list of resolutions for libraries and library services in New Jersey covers seven topic areas: (1) general concerns, including the need for a Library and Information Services Planning Act; (2) broader access to public library services; (3) a statewide cooperative…

  2. IFLA General Conference, 1985. Division on Education and Research. Section: Round Table on Library History. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library history presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The Historical Role of Libraries in Scholarly Communication" (Pamela Spence Richards, Rutgers University, United States); (2) "Current Bibliographies for Library History: An International Review and a Feasibility Study"…

  3. [Library and Information Services for Democracy, for Literacy, and for Productivity]. Conference Briefs.

    ERIC Educational Resources Information Center

    Bobinski, George, Ed.

    1990-01-01

    This issue is designed to provide delegates and other participants in the New York State Governor's Conference in 1990 and the White House Conference on Library and Information Services in July 1991 with an overview of the current status and future needs of libraries and librarianship in the state. Following a message from Mario Cuomo, Governor of…

  4. Libraries and National Development (Final Report of the Third Afro-Nordic Library Conference, Finland, September 3-7 1979).

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific, and Cultural Organization, Paris (France).

    Contained in this collection of presentations from the third Afro-Nordic Library Conference are a foreword describing the objectives of the meeting and 14 papers: "From Oral Tradition to Literary Writing: The Awakening of National Awareness," by Heikki Kirkinen; "African Library Systems," by L. E. Samarasinghe; "Planning of Library…

  5. IFLA General Conference, 1985. Division of General Research Libraries. Section on University and Other General Research Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on resource sharing, university library standards, and authority control that were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Preservation and Resource-Sharing: Conflicting or Complementary?" (J. F. Govan, United States); (2) "Change in the Catalogue in the Context of Library…

  6. IFLA General Conference, 1984. Special Libraries Division. Section on Art Libraries; Section on Music Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The five papers in this collection on art and music environments in developing countries were presented at the 1984 IFLA general conference. (1) In "Developing an Art for Life's Sake," Gavin Jantjes, a black South African artist currently living and working in the United Kingdom, offers personal viewpoints on issues relating to the art of the…

  7. Computers in Libraries Annual Conference (16th, Washington, DC, March 14-16, 2001): Collected Presentations.

    ERIC Educational Resources Information Center

    Nixon, Carol, Comp.

    This book contains the presentations of the 16th annual Computers in Libraries Conference. Contents include: "Creating New Services & Opportunities through Web Databases"; "Influencing Change and Student Learning through Staff Development"; "Top Ten Navigation Tips"; "Library of the Year: Gwinnett County Public Library Sets Strategies for the…

  8. IFLA General Conference, 1986. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This document includes papers on libraries serving the general public which were presented at the 1986 International Federation of Library Associations (IFLA) conference. The first paper, "Annual Reports of Sections and Round Tables of the Division of Libraries Serving the General Public" includes the following annual reports for fiscal year…

  9. Computers in Libraries Annual Conference (17th, Washington, DC, March 13-15, 2002): Collected Presentations.

    ERIC Educational Resources Information Center

    Nixon, Carol, Comp.

    This book contains presentations from the 17th annual Computers in Libraries Conference. Topics covered include: chatting with a librarian; verbots for library Web sites; collaborative IT (Information Technology) planning at Montgomery County Public Library (Maryland); designing a local government taxonomy; Weblogs; new roles for librarians in…

  10. The White House Conference on Library and Information Services (Washington, D.C., July 9-13, 1991).

    ERIC Educational Resources Information Center

    White House Conference on Library and Information Services.

    Designed for use by participants in the 1991 White House Conference on Library and Information Services (WHCLIS2), this guide begins with an overview of the White House Conferences on Library and Information Services, including background information on the 1979 Conference (WHCLIS) and an introduction to the 1991 Conference. Also in the first of…

  11. Libraries and Librarians: Making a Difference in the Knowledge Age. IFLA Council and General Conference: Conference Programme and Proceedings (67th, Boston, Massachusetts, August 16-25, 2001).

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This document contains the proceedings of the IFLA (International Federation of Library Associations and Institutions) 2001 conference, including the conference program and papers, and a list of participants. The theme of the conference was "Libraries and Librarians: Making a Difference in the Knowledge Age." Subthemes included: (1) advancing the…

  12. Library and Information Services for the Public. Proceedings of the Conference of the Papua New Guinea Library Association (8th, Waigani, Port Moresby, October 18-19, 1979).

    ERIC Educational Resources Information Center

    Flanagan, John, Ed.

    The product of a conference concerned with the role of libraries in developing countries, this set of proceedings includes opening comments from the conference; a paper on "Strategies in the Planning of Library and Information Services," by Hedwig Anuar; five papers on the general topic of "Literacy and the Library"; an address entitled "Reaching…

  13. Washington and Beyond: The White House Conference on Library and Information Services.

    ERIC Educational Resources Information Center

    Gell, Marilyn K.

    1979-01-01

    Presents an overview of the White House Conference and its importance to all librarians, noting that it is a chance to examine the individual's information needs and to redefine the role of libraries and information services. (Author/CMV)

  14. The Off-Campus Library Services Conference Proceedings (7th, San Diego, California, October 25-27, 1995).

    ERIC Educational Resources Information Center

    Jacob, Carol J., Comp.

    This conference proceedings of off-campus library services includes 37 papers covering the following topics: ensuring excellence in distance library services; DePaul University's research consultation service; using videos to promote library service; a virtual academic library project in England; student-centered library instruction off campus;…

  15. Nebraska Governor's Conference on Library Services. Proceedings Report (Lincoln, Nebraska, April 27-28, 1976).

    ERIC Educational Resources Information Center

    1976

    This report contains the texts of six speeches, and recommendations of 15 citizen discussion groups from the 1976 Nebraska Conference on Library Services. The theme of the conference, "The Citizen Challenge," and discussion topics are based on concerns expressed by the 1,403 Nebraska citizens who were interviewed in a series of Statewide Hearing…

  16. Statistics in Support of the Ten Topic Areas. White House Conference on Library and Information Services.

    ERIC Educational Resources Information Center

    Keyser, Amy B., Ed.; LaMoure, Lawrence, Ed.

    This booklet presents statistical tables and graphs which were compiled to assist the delegates to the White House Conference on Library and Information Services in formulating their arguments. The tables and graphs have been assigned to one or more of the 10 topic areas, but may also be applied to the three conference themes of Literacy,…

  17. Proceedings of the Governor's Library Conference (1st, Albany, June 24-25, 1965).

    ERIC Educational Resources Information Center

    Governor's Library Conference, Albany, NY.

    An invitational conference, attended by 200 library executives, practitioners and persons from allied fields, was held to explore progress and problems in library development in New York State. Special consideration was given to: (1) the application of data processing techniques for reference and research and (2) the improvement of information…

  18. IFLA General Conference, 1985. Division on Education and Research. Section on Library Theory and Research. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library theory and research presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Information for Self Reliance and Self Determination: The Role of Community Information Services" (Elaine Kempson, Acumen, United Kingdom); (2) "Relationships between Practice, Education and Research in the…

  19. "Fostering Library as Place for Distance Students: Best Practices from Two Universities"--Conference Report

    ERIC Educational Resources Information Center

    Cannady, Rachel E.

    2011-01-01

    In this article, the author reports on the Association of College and Research Libraries (ACRL) Conference which occurred in Philadelphia, Pennsylvania, March 29-April 2, 2011. There were numerous panel sessions to choose from, and the author attended "Fostering Library as Place for Distance Students: Best Practices from Two Universities." In this…

  20. IFLA General Conference, 1985. Division on Management and Technology. Round Tables for the Management of Library Associations, Editors of Library Journals, and Audiovisual Media. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    This document includes papers presented at three round tables at the 1985 International Federation of Library Associations (IFLA) conference. Papers from the Round Table on Management of Library Associations include: (1) "Political Persuasion of Library Associations for the National Development of Libraries. The View of the Scandinavian Library…

  1. IFLA General Conference, 1992. Division of General Research Libraries: Section on National Libraries; Section on Parliamentary Libraries; Section on University Libraries and Other General Research Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    Fifteen papers delivered for the Division of General Research Libraries at the 1992 International Federation of Library Associations and Institutions annual meeting are presented. These papers deal with national libraries, parliamentary (legislative) libraries, and university libraries. The papers are: (1) "Seeking Alternatives to National…

  2. IFLA General Conference, 1989. Division of General Research Libraries. Section of National Libraries; Section of University Libraries and Other General Research Libraries; Section of Parliamentary Libraries. Booklet 10.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    There are 11 papers from the Division of General Research Libraries in this compilation: (1) "National Library and Information Needs: Alternative Ways of Meeting Them, with Special Reference to the Role of National Libraries" (Maurice B. Line); (2) "Managing Change in National LIbraries: The Library of Congress" (James H. Billington); (3) "Coping…

  3. Report from the States, 1985. White House Conference on Library and Information Services Task Force 6th Annual Report.

    ERIC Educational Resources Information Center

    Wells, Gladys Ann, Comp.

    This summary reports the results of the 1985 Survey of States and Territories gathered by the White House Conference on Library and Information Services Task Force (WHCLIST), which focused on library service activities, overall progress made toward the implementation of the resolutions adopted by the 1979 White House Conference on Library and…

  4. IFLA General Conference, 1991. Division of General Research Libraries: Section of National Libraries; Section of University Libraries and Other General Research Libraries; Section of Parliamentary Libraries. Booklet 1.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    The 11 papers in this booklet were presented at meetings of 3 sections within the Division of General Research Libraries: (1) "On the Concept of Development of the National Library of the USSR" (N. I. Tyulina); (2) "Strategic Planning in National Libraries" (Maurice B. Line, UK); (3) "Strategic Planning in National Libraries" (Patricia Donlon,…

  5. The International Dimension. Proceedings of A Pre-Conference Symposium [on] The White House Conference on Library and Information Services. Final Report.

    ERIC Educational Resources Information Center

    White House Conference on Library and Information Services.

    This final report furnishes a summary of the special pre-conference symposium on "White House Conference on Library and Information Services '91: The International Dimension." This symposium focused on the many facets of global library and information services in the light of current needs, governmental programs, private sector initiatives, and…

  6. White House Conference on School Libraries: Keynote Address.

    ERIC Educational Resources Information Center

    Gregorian, Vartan

    2002-01-01

    Discusses the importance of libraries, books, and reading. Highlights include: the role of libraries in our culture; literacy, reading, and writing as the essence of thinking; the relationship between the book and the reader; and the impact of information technologies. (MES)

  7. IFLA General Conference, 1985. Division on Education and Research. Section on Library Schools and Other Training Aspects. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The changing nature of library and information specialists' training and education is addressed in the following papers on library schools presented at the 1985 International Federation of Library Associations (IFLA) conference: (1) "Information Access and the Changing Library School Curriculum" (Herbert S. White, United States); (2)…

  8. IFLA General Conference, 1987. Division of Special Libraries. Biological and Medical Science Libraries Section. Social Science Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Six of the nine papers in this collection focus on biological and medical science libraries; the remaining three are concerned with social science libraries. The papers on biological and medical science libraries appear first in this list: (1) "Standards for Medical and Health Care Libraries: Canada" (Jan Greenwood, Canada); (2) "Standards for…

  9. Sixth Anglo-Scandinavian Public Library Conference on Public Libraries as Cultural Centres, Koli, Finland, 19th - 23rd August, 1970.

    ERIC Educational Resources Information Center

    Rautalin, Marja-Leena, Ed.

    The developing role of the library as a center for cultural activity was the principal subject of discussion at the sixth Anglo-Scandinavian Library Conference. Aspects of this growing function which were treated in participants' papers included: the spectrum of activities which is encompassed by the word "cultural"; the library's role in…

  10. IFLA General Conference, 1987. Division of General Research Libraries. Parliamentary Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The seven papers in this collection describe parliamentary library services and activities in both developed and developing countries: (1) "The Electronic Catalogue of the Library of the Deutscher Bundestag" (Ernst Kohl, West Germany); (2) "Library of the Assembly of the Republic: Modernization Efforts" (A. Cabral, Portugal); (3) "Los Servicios…

  11. Off-Campus Library Services Conference Proceedings (8th, Providence, Rhode Island, April 22-24, 1998).

    ERIC Educational Resources Information Center

    Thomas, P. Steven, Comp.; Jones, Maryhelen, Comp.

    The 27 papers that make up this Proceedings were presented at the Eighth Off-Campus Library Services Conference. The primary objective of the conference was to provide a forum where practitioners involved in library services for off-campus constituents could gather to exchange relevant ideas, concerns and perspectives, and to share research.…

  12. IFLA General Conference, 1987. Division of Special Libraries. Art Libraries Section. Round Table on Audiovisual Media. Administrative Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Seven of the 13 papers in this collection discuss art libraries; the remaining six papers are concerned with administrative and special libraries. The papers from the Section of Art Libraries appear first in this list: (1) "SCOAD (Standing Committee on Art Documentation): Its Inception and Aims" (Julian Gardner, United Kingdom); (2) "The Pictorial…

  13. Conference on Education for Information Science--Strategies for Change in Library School Programs.

    ERIC Educational Resources Information Center

    Klempner, Irving M., Ed.

    1978-01-01

    This proceedings of a conference at the School of Library and Information Science at SUNY-Albany includes two presentations on diagnostics and strategies for change, from the faculty viewpoint and from the administrative viewpoint; three papers on curricular changes and paradigms; and comments by a three-member reactor panel. (MBR)

  14. Reports from the 2011 Missouri Library Association Annual Conference, October 5-7, 2011

    ERIC Educational Resources Information Center

    Miller, Andrea

    2012-01-01

    This article presents reports from the 2011 Missouri Library Association Annual Conference held on October 5-7, 2011. The first report, entitled "Online Information Literacy Instruction: Challenges and Strategies," was presented by Kristine Stewart and Kyle Denlinger, University of Missouri-Columbia. Stewart and Denlinger talk about their…

  15. Library Association of Australia, Proceedings Biennial Conference (15th, Adelaide, 25th-29th August, 1969).

    ERIC Educational Resources Information Center

    Library Association of Australia, Sidney.

    The fifteenth biennial Conference of the Library Association of Australia was held from August 25th to 29th, 1969, in Adelaide. This proceedings volume contains many of the papers given, and summaries of many others. Five papers were presented during the plenary sessions. Thirty-one authors presented general papers. There were nine seminars…

  16. Statements and Remarks Made at the White House Conference on Library and Information Services, November 15-17, 1979.

    ERIC Educational Resources Information Center

    Benton, Charles; And Others

    Remarks made by key speakers of the conference are presented, beginning with an introduction and identification of the five major themes by Charles Benton, Chairman of the National Commission on Libraries and Information Science, and of the conference. Marilyn Gell, Executive Director of the conference, described the services of the White House…

  17. The Off-Campus Library Services Conference Proceedings (6th, Kansas City, Missouri, October 6-8, 1993).

    ERIC Educational Resources Information Center

    Jacob, Carol J., Comp.

    This conference proceedings of off-campus library services includes 30 papers covering the following topics: extracting the hidden potential of course syllabi; obstacles to user education for off-campus students; using Hypercard in an off-campus library environment; satisfying accreditation requirements in lean times; the library's role in…

  18. White House Conference on Library and Information Services Follow-Up Inquiry. State/Territory Agency Responses.

    ERIC Educational Resources Information Center

    National Commission on Libraries and Information Science, Washington, DC.

    State and territory agency responses to a 1980 follow-up inquiry of the 1979 White House Conference on Library and Information Services (WHCLIST) are presented. Individual questionnaires were completed by 24 states and 2 trust territories. Questionnaires provide information on increased state aid to libraries, new library legislation,…

  19. IFLA General Conference, 1992. Presession Seminar on the Status, Reputation, and Image of the Library and Information Profession. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    Seven papers are presented from the presession of the 1992 International Federation of Library Associations and Institutions (IFLA) conference dealing with the status and reputation of the library and information professions, which continue to suffer a lack of image in society. Suggestions for improving the status of the library and information…

  20. IFLA General Conference, 1987. Division of Special Libraries. Science and Technology Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The six papers in this collection focus on science and technology libraries: (1) "Human Aspects of Electronically-Stored Information: The Library User" (A. J. Meadows, United Kingdom); (2) "Untersuchung Menschlicher Aspekte bei den Elektronisch Gespeicherten Informationen: Ansichten des Leiters eines Bibliothekskollektives = Human Aspects of…

  1. Guidelines for Holding a Governor's Conference on Libraries: A Step-by-Step How to Do It Manual. ALTA Publication Number Three.

    ERIC Educational Resources Information Center

    Lynch, Mrs. Weldon

    This document is a revision of "Guidelines for Holding a Governor's Conference on Libraries (American Library Association, American Trustee Association, 1963). The report discusses what governor's conferences on libraries are and how they may be helpful. Many aspects of planning for such a conference are presented, including how much time to leave…

  2. Libraries for Life: Democracy, Diversity, Delivery. IFLA Council and General Conference: Conference Programme and Proceedings (68th, Glasgow, Scotland, August 18-24, 2002).

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This document presents the program and proceedings from the 68th International Federation of Library Associations and Institutions (IFLA) Conference held in Glasgow, Scotland, August 18-24, 2002. Topics of presentations include: library services for parliaments; needs assessment; the effects of September 11th on information provision and privacy;…

  3. Libraries--Link To Learning. Final Report on the Indiana Governor's Conference on Libraries and Information Services (2nd, Indianapolis, Indiana, November 16-18, 1990).

    ERIC Educational Resources Information Center

    Henson, Jane E., Ed.

    This report focuses on the three themes of the 1991 White House Conference on Library and Information Services, i.e., Democracy, Literacy, and Productivity. The report begins with an introduction by State Librarian Ray Ewick and a summary of the conference activities. Executive summaries of remarks made by speakers during three general conference…

  4. Construction and Screening of Antigen Targeted Immune Yeast Surface Display Antibody Libraries

    SciTech Connect

    Miller, Keith D.; Pefaur, Noah B.; Baird, Cheryl L.

    2008-07-01

    These protocols describe a yeast surface display-based process for the rapid selection of antibodies from immunized mice, eliminating the need for creating and screening hybridoma fusions. A yeast surface display library of single-chain antibody fragments (scFvs) is created from antigen-binding B cells from the splenocytes of immunized mice. The antigen targeted library is then screened for antigen specific scFv by magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS). Library construction and screening can be accomplished in as little as 2 weeks resulting in a panel of scFvs specific for the target antigen.

  5. Construction and Screening of Antigen Targeted Immune Yeast Surface Display Antibody Libraries

    SciTech Connect

    Miller, Keith D.; Pefaur, Noah B.; Baird, Cheryl L.

    2009-08-02

    These protocols describe a yeast surface display-based process for the rapid selection of antibodies from immunized mice, eliminating the need for creating and screening hybridoma fusions. A yeast surface display library of single-chain antibody fragments (scFvs) is created from antigen-binding B cells from the splenocytes of immunized mice. The antigen targeted library is then screened for antigen specific scFv by magneticactivated cell sorting (MACS) and fluorescence-activated cell sorting (FACS). Library construction and screening can be accomplished in as little as 2 weeks, resulting in a panel of scFvs specific for the target antigen.

  6. Libraries Without Walls 4: The Delivery of Library Services to Distant Users. Proceedings of an International Conference Organized by the Centre for Research in Library and Information Management (CERLIM), Manchester Metropolitan University (Manchester, England, September 14-18, 2001).

    ERIC Educational Resources Information Center

    Brophy, Peter, Ed.; Fisher, Shelagh, Ed.; Clarke, Zoe, Ed.

    This proceedings of the fourth Libraries Without Walls Conference addresses key strategic issues arising from international, regional, and cross-sectoral approaches to the provision of library services to distant users. The following papers are included: Theme 1: Libraries and Virtual Learning Environments: "Introduction" (Peter Brophy, Shelagh…

  7. IFLA General Conference, 1985. Division on Libraries Serving the General Public. Round Table on Library Service to Ethnic and Linguistic Minorities. Papers. the General Public. Round Table on Library Service to Ethnic and Linguistic Minorities. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library services to ethnic and linguistic minorities which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "On Serving the Older Polyglot: The Role of the Public Libraries in the United States" (Sylva N. Manoogian, United States); (2) "Minority Language Selection and Acquisition…

  8. Autopolyreactivity Confers a Holistic Role in the Immune System.

    PubMed

    Avrameas, S

    2016-04-01

    In this review, we summarize and discuss some key findings from the study of naturally occurring autoantibodies. The B-cell compartment of the immune system appears to recognize almost all endogenous and environmental antigens. This ability is accomplished principally through autopolyreactive humoral and cellular immune receptors. This extended autopolyreactivity (1) along immunoglobulin gene recombination contributes to the immune system's ability to recognize a very large number of self and non-self constituents; and (2) generates a vast immune network that creates communication channels between the organism's interior and exterior. Thus, the immune system continuously evolves depending on the internal and external stimuli it encounters. Furthermore, this far-reaching network's existence implies activities resembling those of classical biological factors or activities that modulate the function of other classical biological factors. A few such antibodies have already been found. Another important concept is that natural autoantibodies are highly dependent on the presence or absence of commensal microbes in the organism. These results are in line with past and recent findings showing the fundamental influence of the microbiota on proper immune system development, and necessitate the existence of a host-microbe homeostasis. This homeostasis requires that the participating humoral and cellular receptors are able to recognize self-antigens and commensal microbes without damaging them. Autopolyreactive immune receptors expressing low affinity for both types of antigens fulfil this role. The immune system appears to play a holistic role similar to that of the nervous system.

  9. Brick & Click Libraries: An Academic Library Conference (14th, Maryville, Missouri, November 7, 2014)

    ERIC Educational Resources Information Center

    Baudino, Frank, Ed.; Johnson, Carolyn, Ed.

    2014-01-01

    Eighteen scholarly papers and twelve abstracts comprise the content of the fourteenth annual Brick and Click Libraries Symposium, held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship.…

  10. IFLA General Conference, 1987. Division of Special Libraries. Geography and Map Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The four papers in this collection report on geography and map libraries: (1) "Changing Patterns of Map Availability" (C. R. Perkins and R. B. Parry, United Kingdom), which presents the results of a three year survey into the state of world mapping, a discussion of the implications of changing patterns of map availability for map library…

  11. Economic Vitality: Challenge and Opportunity. Selected Readings from the Fall Conference of the Kansas Library Association: Public Library Section (Hutchinson, Kansas, September 28-29, 1988). Monograph Series Number 9.

    ERIC Educational Resources Information Center

    Emporia State Univ., KS. School of Library and Information Management.

    In September 1988, the Public Library/Trustees Section of the Kansas Library Association, with the support of the School of Library and Information Management at Emporia State University and the Kansas State Library, held a conference on economic vitality. Following a "Review and Preview" by Martha Hale, Dean of the School of Library and…

  12. IFLA General Conference, 1986. Pre-Session Seminar on Special Libraries and Their Role in National Development. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    A seminar on special libraries and their role in national development was held as a pre-session before the IFLA conference in 1986. Papers presented include: (1) "On the Problem of Document Delivery in the Field of Science and Technology in Asia" (Daxun He, People's Republic of China); (2) "The Planning and Development of Special Library and…

  13. Conference on Inter-Library Cooperation and Exchange, San Juan, Puerto Rico, April 30 - May 2, 1969.

    ERIC Educational Resources Information Center

    Virgin Islands Dept. of Conservation and Cultural Affairs, St. Thomas. Div. of Libraries and Museums.

    The Conference had as its main objective the assembling of library administrators and librarians to consider all aspects relating to the implementation of a library cooperative plan for the interchange of publications, photoduplication services and bibliographic information. Papers that were presented are: (1) "Automation and Bibliographic…

  14. Identifying Liaison Opportunities through Content Analysis: Academic Library Trends in the Ecological Society of America's Conference Program

    ERIC Educational Resources Information Center

    Conklin, Jamie L.

    2013-01-01

    Science and technology librarians need to continually invest time into professional development activities to gain new skills relevant to the faculty and students they serve. Many academic libraries face diminishing budgets and have few travel dollars available for attendance at library and subject-specific conferences. This study determined that…

  15. IFLA General Conference, 1989. Division of Libraries Serving the General Public. Section on Children's Libraries; Section of School Libraries; Section of Libraries Serving Disadvantaged Persons. Booklet 30.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Fifteen papers from the Division of Libraries Serving the General Public are included in this collection: "When the Children's Library Meets the Museum" (French and English versions; Annie Pissard); "Value of Library Service for Children Literature in France" (Aline Eisenegger); "The Latin American Literature in France" (Aline Eisenegger); "The…

  16. The evolution of secondary organization in immune system gene libraries

    SciTech Connect

    Hightower, R.; Forrest, S.; Perelson, A.S.

    1993-02-01

    A binary model of the immune system is used to study the effects of evolution on the genetic encoding for antibody molecules. We report experiments which show that the evolution of immune system genes, simulated by the genetic algorithm, can induce a high degree of genetic organization even though that organization is not explicitly required by the fitness function. This secondary organization is related to the true fitness of an individual, in contrast to the sampled fitness which is the explicit fitness measure used to drive the process of evolution.

  17. The evolution of secondary organization in immune system gene libraries

    SciTech Connect

    Hightower, R.; Forrest, S. . Dept. of Computer Science); Perelson, A.S. )

    1993-01-01

    A binary model of the immune system is used to study the effects of evolution on the genetic encoding for antibody molecules. We report experiments which show that the evolution of immune system genes, simulated by the genetic algorithm, can induce a high degree of genetic organization even though that organization is not explicitly required by the fitness function. This secondary organization is related to the true fitness of an individual, in contrast to the sampled fitness which is the explicit fitness measure used to drive the process of evolution.

  18. Laura Bush: Papers Presented at the White House on the Occasion of the White House Conference on School Libraries, June 4, 2002.

    ERIC Educational Resources Information Center

    Bush, Laura; Martin, Robert; Gregorian, Vartan; Neuman, Susan; Lance, Keith Curry; Kimsey-Pharr, Faye; Hartzell, Gary; Smith, Kathleen D.; Wisely, Steve; DeVita, M. Christine

    2002-01-01

    Includes nine papers that were presented at the White House Conference on School Libraries in 2002. Highlights include the importance of books and access to information in today's society; the role of school librarians; the importance of school libraries; how to change perceptions of school libraries; and how school libraries can improve student…

  19. IFLA General Conference, 1990. Division of Special Libraries: Section of Administrative Libraries; Section of Social Science Libraries; Section of Science and Technology Libraries; Section of Biological and Medical Sciences Libraries; Section of Art Libraries. Booklet 2.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 23 papers in this collection were presented at 5 sections of the Division of Special Libraries: (1) "Principles of Government Librarianship" (Hans H. van der Neut); (2) "Strategic Planning as an Instrument of Improving Library Quality" (Maurice B. Line); (3) "Library Staff Development Consultancy: A Means to Achieve a Better Library?"…

  20. IFLA General Conference 1988. Division of General Research Libraries. Section on National Libraries; Section on Parliamentary Libraries; Section on University Libraries and Other General Research Libraries; Section on Library History.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 12 papers in this compilation focus on the activities of general research libraries, i.e., national, parliamentary, and university libraries: (1) "Commercial and Revenue Raising Activities in National Libraries" (Maurice Line & Peter Scott, New Zealand); (2) "The End of All and Forever--On the Acquisition Policies of National Libraries and the…

  1. IFLA General Conference, 1986. Pre-Conference Seminar on Automated Systems for Access to Multilingual and Multiscript Library materials: Problems and Solutions. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    A seminar which considered problems and solutions regarding automated systems for access to multilingual and multiscript library materials was held as a pre-session before the IFLA general conference in 1986. Papers presented include: (1) "Romanized and Transliterated Databases of Asian Language Materials--History, Problems, and Prospects" (Suzine…

  2. Academic Libraries: Myths and Realities. Proceedings of the National Conference of the Association of College and Research Libraries (3rd, Seattle, Washington, April 4-7, 1984).

    ERIC Educational Resources Information Center

    Dodson, Suzanne C., Ed.; Menges, Gary L., Ed.

    The 56 papers in this proceedings are divided into three groups--theme, alternative format, and contributed papers. Myths and realities are explored in the first group as they relate to university, college, community, and junior college libraries, and to private sector information utilities. The conference wrap-up session is included in this…

  3. CD31 signals confer immune privilege to the vascular endothelium

    PubMed Central

    Cheung, Kenneth; Ma, Liang; Wang, Guosu; Coe, David; Ferro, Riccardo; Falasca, Marco; Buckley, Christopher D.; Mauro, Claudio; Marelli-Berg, Federica M.

    2015-01-01

    Constitutive resistance to cell death induced by inflammatory stimuli activating the extrinsic pathway of apoptosis is a key feature of vascular endothelial cells (ECs). Although this property is central to the maintenance of the endothelial barrier during inflammation, the molecular mechanisms of EC protection from cell-extrinsic, proapoptotic stimuli have not been investigated. We show that the Ig-family member CD31, which is expressed by endothelial but not epithelial cells, is necessary to prevent EC death induced by TNF-α and cytotoxic T lymphocytes in vitro. Combined quantitative RT-PCR array and biochemical analysis show that, upon the engagement of the TNF receptor with TNF-α on ECs, CD31 becomes activated and, in turn, counteracts the proapoptotic transcriptional program induced by TNF-α via activation of the Erk/Akt pathway. Specifically, Akt activation by CD31 signals prevents the localization of the forkhead transcription factor FoxO3 to the nucleus, thus inhibiting transcription of the proapoptotic genes CD95/Fas and caspase 7 and de-repressing the expression of the antiapoptotic gene cFlar. Both CD31 intracellular immunoreceptor tyrosine-based inhibition motifs are required for its prosurvival function. In vivo, CD31 gene transfer is sufficient to recapitulate the cytoprotective mechanisms in CD31− pancreatic β cells, which become resistant to immune-mediated rejection when grafted in fully allogeneic recipients. PMID:26392551

  4. Proceedings of the National Conference on Population Library and Information Services (3rd Chapel Hill, N. C., May 14-15, 1970)

    ERIC Educational Resources Information Center

    Kellermann, Priscilla

    Contains the proceedings of the Third National Conference on Population Library and Information Services held in Chapel Hill, North Carolina, May 14-15, 1970. Under the joint sponsorship of the Carolina Population Center and the Population Council of New York, this was the third in a series of annual conference/workshops devoted to library and…

  5. Tetanus vaccination with a dissolving microneedle patch confers protective immune responses in pregnancy.

    PubMed

    Esser, E Stein; Romanyuk, AndreyA; Vassilieva, Elena V; Jacob, Joshy; Prausnitz, Mark R; Compans, Richard W; Skountzou, Ioanna

    2016-08-28

    Maternal and neonatal tetanus claim tens of thousands lives every year in developing countries, but could be prevented by hygienic practices and improved immunization of pregnant women. This study tested the hypothesis that skin vaccination can overcome the immunologically transformed state of pregnancy and enhance protective immunity to tetanus in mothers and their newborns. To achieve this goal, we developed microneedle patches (MNPs) that efficiently delivered unadjuvanted tetanus toxoid to skin of pregnant mice and demonstrated that this route induced superior immune responses in female mice conferring 100% survival to tetanus toxin challenge when compared to intramuscular vaccination. Mice born to MNP-vaccinated mothers showed detectable tetanus-specific IgG antibodies up to 12weeks of age and complete protection to tetanus toxin challenge up at 6weeks of age. In contrast, none of the 6-week old mice born to intramuscularly vaccinated mothers survived challenge. Although pregnant mice vaccinated with unadjuvanted tetanus toxoid had 30% lower IgG and IgG1 titers than mice vaccinated intramuscularly with Alum®-adjuvanted tetanus toxoid vaccine, IgG2a titers and antibody affinity maturation were similar between these groups. We conclude that skin immunization with MNPs containing unadjuvanted tetanus toxoid can confer potent protective efficacy to mothers and their offspring using a delivery method well suited for expanding vaccination coverage in developing countries. PMID:27327766

  6. Tetanus vaccination with a dissolving microneedle patch confers protective immune responses in pregnancy.

    PubMed

    Esser, E Stein; Romanyuk, AndreyA; Vassilieva, Elena V; Jacob, Joshy; Prausnitz, Mark R; Compans, Richard W; Skountzou, Ioanna

    2016-08-28

    Maternal and neonatal tetanus claim tens of thousands lives every year in developing countries, but could be prevented by hygienic practices and improved immunization of pregnant women. This study tested the hypothesis that skin vaccination can overcome the immunologically transformed state of pregnancy and enhance protective immunity to tetanus in mothers and their newborns. To achieve this goal, we developed microneedle patches (MNPs) that efficiently delivered unadjuvanted tetanus toxoid to skin of pregnant mice and demonstrated that this route induced superior immune responses in female mice conferring 100% survival to tetanus toxin challenge when compared to intramuscular vaccination. Mice born to MNP-vaccinated mothers showed detectable tetanus-specific IgG antibodies up to 12weeks of age and complete protection to tetanus toxin challenge up at 6weeks of age. In contrast, none of the 6-week old mice born to intramuscularly vaccinated mothers survived challenge. Although pregnant mice vaccinated with unadjuvanted tetanus toxoid had 30% lower IgG and IgG1 titers than mice vaccinated intramuscularly with Alum®-adjuvanted tetanus toxoid vaccine, IgG2a titers and antibody affinity maturation were similar between these groups. We conclude that skin immunization with MNPs containing unadjuvanted tetanus toxoid can confer potent protective efficacy to mothers and their offspring using a delivery method well suited for expanding vaccination coverage in developing countries.

  7. IFLA General Conference 1988. Open Forum on IFLA Core Programs; Section on INTAMEL; Libraries Serving Disadvantaged Persons; Libraries for the Blind; Children's Libraries; School Libraries.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Twenty-one papers from the Open Forum on Core Programs are included in this collection: (1) "The IFLA (International Federal of Library Associations) UAP (Universal Availability of Publications) Programme" (M. F. Plassard, IFLA Programme Officer); (2) "Progress Report on IFLA Core Programme for Universal Dataflow and Telecommunications (UDT)" (L.…

  8. IFLA General Conference, 1987. Division of General Research Libraries. National Libraries Section. University Libraries and Other General Research Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Of the 11 papers in this collection, three are in German and in English translation; one is in French with an English translation and two are in French only; one is in Spanish with an English translation; and four are in English. The papers include: (1) "Europaische Bibliotheksinitiativen = European Library Initiatives" (Gunther Pflug); (2) "Die…

  9. Education in Library and Information Science. Proceedings of the International Conference (Dubrovnik, Yugoslavia, May 21-26, 1984).

    ERIC Educational Resources Information Center

    Mihel, Ivan, Ed.; Tudor-Silovic, Neva, Ed.

    1984-01-01

    An international conference attended by 59 participants from 12 countries was organized to present Yugoslavia as a case study to the international audience, to bring to the Yugoslav audience a variety of international experiences in library and information science education and training, and to acquaint participants with some of the new…

  10. Proceedings of the Conference on Application of Scanning Methodologies in Libraries (Beltsville, Maryland, November 17-18, 1988).

    ERIC Educational Resources Information Center

    Blamberg, Donald L., Ed.; And Others

    Planned and organized by the National Agricultural Library (NAL), the conference was designed to bring together people involved in scanning projects and to evaluate the feasibility of using hand-held scanning devices for transcribing bibliographic information during the cataloging and indexing processes. Ten previously unpublished papers and a…

  11. Information: Books are Just the Beginning. The Michigan White House Conference on Libraries and Information Services. A Report.

    ERIC Educational Resources Information Center

    Michigan State Dept. of Education, Lansing.

    This report details the proceedings of the Michigan pre-White House Conference on Libraries and Information Services held from March 26-28, 1979. Demonstrations, displays, discussions, elections, and participants are described, and the 68 resolutions arising from the discussions are listed according to the following topics: (1) finance, (2)…

  12. Federal Pre-White House Conference on Library and Information Services Proceedings (Bethesda, Maryland, November 26-27, 1990).

    ERIC Educational Resources Information Center

    Federal Library and Information Center Committee, Washington, DC.

    This report presents the recommendations and proceedings of the Federal Pre-White House Conference on Library and Information Services (WHCLIS II) in two sections. Thirteen issues and recommendations of high national priority identified by the federal delegates to the WHCLIS II are provided in the first section: (1) Preservation Plan for Federal…

  13. IFLA General Conference, 1984. Education and Research Division. Section on Library Schools and Other Training Aspects. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on the education and training of librarians presented at the 1984 IFLA general conference include: (1) "Training and Developing a Professional for the Widening Library Responsibilities: A Management View," in which Charles K. Wambugu (Kenya) examines the management requirements for a professional librarian and discusses the role of library…

  14. Galectin-1 confers immune privilege to human trophoblast: implications in recurrent fetal loss.

    PubMed

    Ramhorst, Rosanna E; Giribaldi, Laura; Fraccaroli, Laura; Toscano, Marta A; Stupirski, Juan C; Romero, Marta D; Durand, Edith S; Rubinstein, Natalia; Blaschitz, Astrid; Sedlmayr, Peter; Genti-Raimondi, Susana; Fainboim, Leonardo; Rabinovich, Gabriel A

    2012-10-01

    Mechanisms accounting for the protection of the fetal semi-allograft from maternal immune cells remain incompletely understood. In previous studies, we showed that galectin-1 (Gal1), an immunoregulatory glycan-binding protein, hierarchically triggers a cascade of tolerogenic events at the mouse fetomaternal interface. Here, we show that Gal1 confers immune privilege to human trophoblast cells through the modulation of a number of regulatory mechanisms. Gal1 was mainly expressed in invasive extravillous trophoblast cells of human first trimester and term placenta in direct contact with maternal tissue. Expression of Gal1 by the human trophoblast cell line JEG-3 was primarily controlled by progesterone and pro-inflammatory cytokines and impaired T-cell responses by limiting T cell viability, suppressing the secretion of Th1-type cytokines and favoring the expansion of CD4(+)CD25(+)FoxP3(+) regulatory T (T(reg)) cells. Targeted inhibition of Gal1 expression through antibody (Ab)-mediated blockade, addition of the specific disaccharide lactose or retroviral-mediated siRNA strategies prevented these immunoregulatory effects. Consistent with a homeostatic role of endogenous Gal1, patients with recurrent pregnancy loss showed considerably lower levels of circulating Gal1 and had higher frequency of anti-Gal1 auto-Abs in their sera compared with fertile women. Thus, endogenous Gal1 confers immune privilege to human trophoblast cells by triggering a broad tolerogenic program with potential implications in threatened pregnancies. PMID:22752006

  15. RESET: Information Services Trends in 2012. Selected Papers from PIALA Conference 2012, Pacific Islands Association of Libraries, Archives, and Museums Annual Conference (22nd, Tumon, U.S. Territory of Guam, Nov 13-16, 2012)

    ERIC Educational Resources Information Center

    Drake, Paul B., Ed.

    2013-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 22th annual conference was held in Tumon, Territory of Guam USA, November 13-16, 2012. The volume begins with a listing of the members of the PIALA 2012 Planning Committee and…

  16. "Be Informed, Stay Connected, Community Transformation!" Selected Papers from the PIALA Conference 2014, Pacific Islands Association of Libraries, Archives, and Museums Annual Conference (24th, Koror, Republic of Palau, Nov 10-15, 2014)

    ERIC Educational Resources Information Center

    Drake, Paul Burton

    2015-01-01

    This publication follows the tradition of publishing selected papers from the Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 24th annual conference was held in Koror, Republic of Palau, November 10-15, 2014. This volume includes a listing of the PIALA 2014 Organizing Committee and PIALA Officers and…

  17. Discover the Hidden Jewels in Your Library and Sharing the Wealth through Collaboration. Selected Papers from PIALA 2011, Pacific Islands Association of Libraries, Archives, and Museums Annual Conference (21st, Kosrae, Federated States of Micronesia, November 14-17, 2011)

    ERIC Educational Resources Information Center

    Drake, Paul B., Ed.

    2012-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 21st annual conference was held in Kosrae, Federated States of Micronesia, November 14-17, 2011. The volume begins with a listing of the members of the PIALA 2011 Planning…

  18. Pacific Visions: Finding, Selecting, and Using Resources for Your Libraries, Archives, and Museums. Selected Papers from PIALA 2009, Pacific Islands Association of Libraries, Archives, and Museums Annual Conference (19th, Pohnpei, Federated States of Micronesia, November 16-21, 2009)

    ERIC Educational Resources Information Center

    Drake, Paul B., Ed.

    2011-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 19th annual conference was held in Pohnpei, Federated States of Micronesia, November 16-21, 2009. The volume begins with a listing of the members of the PIALA 2009 Planning…

  19. IFLA General Conference 1988. Division of Special Libraries. Section on Science and Technology Libraries; Section on Art Libraries; Section on Social Science Libraries; Section on Geography and Map Libraries; Division of Contributed Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 23 papers in this collection include papers on special libraries and miscellaneous contributed papers: (1) "Networking Potentialities and Limitations--Special Library Networks in Socialist Countries--An Overview, and the Main Ways of Perestroika in the Work of Scientific and Technical Libraries at the Present Stage" (D. Schmidmaier & I.…

  20. IFLA General Conference, 1990. Division of General Research Libraries: Open Forum; Section of National Libraries; Section of University Libraries and Other General Research Libraries; Section of Parliamentary Libraries. Booklet 1.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 16 reports in this collection were presented at an open forum on research libraries and meetings of three more specialized sections: (1) "Nature and Purpose of the Division of General Research Libraries" (Ernst Kohl); (2) "Objectives and Goals of the Section of Parliamentary Libraries" (Ernst Kohl); (3) "The IFLA (International Federation of…

  1. IFLA General Conference, 1992. Division of Special Libraries: Section on Art Libraries; Section on Geography and Map Libraries; Section on Government Libraries; Section on Science and Technology Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    The following 21 papers were delivered for the Special Libraries Division of the International Federation of Library Associations and Institutions at its 1992 annual meeting: (1) "From Indochina to Afghanistan: Arts from Abroad in Parisian Libraries" (M. F. Macouin); (2) "The Indonesian Archeology Photograph and Documentation System (IAPDS) in…

  2. The Future of Libraries in Human Communication: Abstracts and Fulltext Documents of Papers and Demos Given at the [International Association of Technological University Libraries] IATUL Conference (Chania, Greece, May 17-21, 1999). Volume 19.

    ERIC Educational Resources Information Center

    International Association of Technological Univ. Libraries, Gothenburg (Sweden).

    This proceedings of the IATUL 1999 conference of the contains the following papers: "From Industry to Higher Education and Libraries: Building the Fast Response Library" (A. S. Apostolou & C. H. Skiadas); "Managing the Learning Agenda in a Converged Service Environment" (Richard Biddiscombe); "Management of Electronic Information" (Michael…

  3. George Orwell &"Nineteen Eighty-Four": The Man and the Book. A Conference at the Library of Congress, April 30 and May 1, 1984.

    ERIC Educational Resources Information Center

    Library of Congress, Washington, DC.

    Proceedings of a conference of international Orwell experts gathered at the Library of Congress are presented in this collection. The collection is divided into four sections, corresponding with the four conference sessions: "What Orwell Really Wrote"; "Orwell: The Man"; "'Nineteen Eighty-Four': The Book"; and "'Nineteen Eighty-Four': Its Meaning…

  4. The Management of Technical Innovation in Libraries. Proceedings of a Conference (London, England, April 23, 1980).

    ERIC Educational Resources Information Center

    Loughborough Univ. of Technology (England). Dept. of Library and Information Studies.

    These four papers presented at the Polytechnic of Central London School of Management focus on the management of technical innovation in libraries as it affects decision making, library organization, non-systems staff, and interlibrary cooperation. Procedures for implementing automation in a library are outlined: a system analysis and costing of…

  5. IFLA General Conference, 1987. Division of Libraries Serving the General Public. Libraries Serving Disadvantaged Persons Section. Library Services to Multicultural Populations Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Eleven of the 15 papers in this collection discuss library services for disadvantaged persons; the remaining four papers are concerned with services to multicultural populations. The papers from the Section of Libraries Serving Disadvantaged Persons appear first in this list: (1) "The Development and Future of Easy Readers for Adults in the UK"…

  6. IFLA General Conference, 1989. Division of Special Libraries. Section of Administrative Libraries; Section of Social Science Libraries; Section of Geography and Map Libraries; Section of Science and Technology Libraries; Section of Biological and Medical Science Libraries; Section of Art Libraries; Joint Session with the Round Table on Audiovisual Media. The International Association of Law Libraries. Booklet 20.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Twenty-one papers from the Division of Special Libraries are included in this collection: "Information Systems Planning as a Tool of Developing Library Work: The Case of Statistics Library" (Heli Myllys); "The Libraries of the French Central Government Departments and the Administrative Library of the City of Paris" (French and English versions;…

  7. Library+

    ERIC Educational Resources Information Center

    Merrill, Alex

    2011-01-01

    This article discusses possible future directions for academic libraries in the post Web/Library 2.0 world. These possible directions include areas such as data literacy, linked data sets, and opportunities for libraries in support of digital humanities. The author provides a brief sketch of the background information regarding the topics and…

  8. Tetanus Neurotoxin Neutralizing Antibodies Screened from a Human Immune scFv Antibody Phage Display Library.

    PubMed

    Wang, Han; Yu, Rui; Fang, Ting; Yu, Ting; Chi, Xiangyang; Zhang, Xiaopeng; Liu, Shuling; Fu, Ling; Yu, Changming; Chen, Wei

    2016-01-01

    Tetanus neurotoxin (TeNT) produced by Clostridium tetani is one of the most poisonous protein substances. Neutralizing antibodies against TeNT can effectively prevent and cure toxicosis. Using purified Hc fragments of TeNT (TeNT-Hc) as an antigen, three specific neutralizing antibody clones recognizing different epitopes were selected from a human immune scFv antibody phage display library. The three antibodies (2-7G, 2-2D, and S-4-7H) can effectively inhibit the binding between TeNT-Hc and differentiated PC-12 cells in vitro. Moreover, 2-7G inhibited TeNT-Hc binding to the receptor via carbohydrate-binding sites of the W pocket while 2-2D and S-4-7H inhibited binding of the R pocket. Although no single mAb completely protected mice from the toxin, they could both prolong survival when challenged with 20 LD50s (50% of the lethal dose) of TeNT. When used together, the mAbs completely neutralized 1000 LD50s/mg Ab, indicating their high neutralizing potency in vivo. Antibodies recognizing different carbohydrate-binding pockets could have higher synergistic toxin neutralization activities than those that recognize the same pockets. These results could lead to further production of neutralizing antibody drugs against TeNT and indicate that using TeNT-Hc as an antigen for screening human antibodies for TeNT intoxication therapy from human immune antibody library was convenient and effective. PMID:27626445

  9. Tetanus Neurotoxin Neutralizing Antibodies Screened from a Human Immune scFv Antibody Phage Display Library

    PubMed Central

    Wang, Han; Yu, Rui; Fang, Ting; Yu, Ting; Chi, Xiangyang; Zhang, Xiaopeng; Liu, Shuling; Fu, Ling; Yu, Changming; Chen, Wei

    2016-01-01

    Tetanus neurotoxin (TeNT) produced by Clostridium tetani is one of the most poisonous protein substances. Neutralizing antibodies against TeNT can effectively prevent and cure toxicosis. Using purified Hc fragments of TeNT (TeNT-Hc) as an antigen, three specific neutralizing antibody clones recognizing different epitopes were selected from a human immune scFv antibody phage display library. The three antibodies (2-7G, 2-2D, and S-4-7H) can effectively inhibit the binding between TeNT-Hc and differentiated PC-12 cells in vitro. Moreover, 2-7G inhibited TeNT-Hc binding to the receptor via carbohydrate-binding sites of the W pocket while 2-2D and S-4-7H inhibited binding of the R pocket. Although no single mAb completely protected mice from the toxin, they could both prolong survival when challenged with 20 LD50s (50% of the lethal dose) of TeNT. When used together, the mAbs completely neutralized 1000 LD50s/mg Ab, indicating their high neutralizing potency in vivo. Antibodies recognizing different carbohydrate-binding pockets could have higher synergistic toxin neutralization activities than those that recognize the same pockets. These results could lead to further production of neutralizing antibody drugs against TeNT and indicate that using TeNT-Hc as an antigen for screening human antibodies for TeNT intoxication therapy from human immune antibody library was convenient and effective. PMID:27626445

  10. Writer to Writer: How To Conference Young Authors. The Bill Harp Professional Teachers Library Series.

    ERIC Educational Resources Information Center

    Thomason, Tommy

    Written for the busy teacher, this book examines classroom writing conferences, one of the most talked about and least practiced strategies in the writing process classroom. Each brief chapter introduces a different facet of the writing conference or the philosophy and classroom practice that undergird it, and each chapter ends with a "something…

  11. Inaugural conference of the International Association of Immunization Managers (IAIM), Istanbul Turkey, 3-4 March 2015.

    PubMed

    Carrasco, Peter; Franco-Paredes, Carlos; Andrus, Jon; Waller, Katie; Maassen, Alison; Symenouh, Emi; Hafalia, Gabrielle

    2015-08-01

    For more than 35 years, most national immunization programs have established managerial structures and processes for delivering vaccination services to their populations. These days, immunization managers are facing an increasing number of challenges due to the introduction of new vaccines, shifting demographic patterns, complex networks of service providers, and maintaining the gains achieved with previous vaccination efforts. To confront these challenges, better program performance will require better managerial practices, which incorporates new technologies. To that end, the International Association of Immunization Managers (IAIM) is the first global professional association launched to promote superior leadership and management skills among health professionals involved with vaccination efforts worldwide. From 3 to 4 March 2015, approximately 132 members from 70 countries representing six regions, gathered in Istanbul, Turkey for the inaugural conference of IAIM. In the two-day program, members selected thirteen peers to constitute the Governing Council. The 12 articles of the bylaws of the Association were also ratified. This conference was a forum for sharing managerial best practices through networking sessions, breakout sessions, and presentations. Members also learned about IAIM sponsored training opportunities to deepen their managerial competencies through peer-to-peer exchanges and scholarship training programs. We believe that the IAIM inaugural conference was an appropriate platform for equipping managers with tools and professional network of peers to support them in achieving national, regional and global immunization goals, including those of the Global Vaccine Action Plan of the World Health Organization.

  12. The Challenge To Be Relevant in the 21st Century: Abstracts and Full Text Documents of Papers and Demos Given at the [International Association of Technological University Libraries] IATUL Conference (Pretoria, South Africa, June 1-5, 1998), Volume 18.

    ERIC Educational Resources Information Center

    International Association of Technological Univ. Libraries, Gothenburg (Sweden).

    This proceedings of the 1998 conference of the International Association of Technological University Libraries (IATUL) contains the full text of the following papers: "A Library Ready for 21st Century Services: The Case of the University of Science and Technology (UST) Library, Kumasi, Ghana" (Helena Rebecca Asamoah-Hassan); "Libraries in…

  13. Immune suppressor factor confers stromal cell line with enhanced supporting activity for hematopoietic stem cells

    SciTech Connect

    Nakajima, Hideaki . E-mail: hnakajim@ims.u-tokyo.ac.jp; Shibata, Fumi; Fukuchi, Yumi; Goto-Koshino, Yuko; Ito, Miyuki; Urano, Atsushi; Nakahata, Tatsutoshi; Aburatani, Hiroyuki; Kitamura, Toshio

    2006-02-03

    Immune suppressor factor (ISF) is a subunit of the vacuolar ATPase proton pump. We earlier identified a short form of ISF (ShIF) as a stroma-derived factor that supports cytokine-independent growth of mutant Ba/F3 cells. Here, we report that ISF/ShIF supports self-renewal and expansion of primary hematopoietic stem cells (HSCs). Co-culture of murine bone marrow cells with a stromal cell line overexpressing ISF or ShIF (MS10/ISF or MS10/ShIF) not only enhanced their colony-forming activity and the numbers of long-term culture initiating cells, but also maintained the competitive repopulating activity of HSC. This stem cell supporting activity depended on the proton-transfer function of ISF/ShIF. Gene expression analysis of ISF/ShIF-transfected cell lines revealed down-regulation of secreted frizzled-related protein-1 and tissue inhibitor of metalloproteinase-3, and the restoration of their expressions in MS10/ISF cells partially reversed its enhanced LTC-IC supporting activity to a normal level. These results suggest that ISF/ShIF confers stromal cells with enhanced supporting activities for HSCs by modulating Wnt-activity and the extracellular matrix.

  14. Intravaginal and intranasal immunizations are equally effective in inducing vaginal antibodies and conferring protection against vaginal candidiasis.

    PubMed

    De Bernardis, Flavia; Boccanera, Maria; Adriani, Daniela; Girolamo, Antonietta; Cassone, Antonio

    2002-05-01

    Oophorectomized, estrogen-treated rats were immunized by the intravaginal or intranasal route with a mannoprotein extract (MP) or secreted aspartyl proteinases (Sap) of Candida albicans, with or without cholera toxin as a mucosal adjuvant. Both routes of immunization were equally effective in (i) inducing anti-MP and anti-Sap vaginal antibodies and (ii) conferring a high degree of protection against the vaginal infection by the fungus. These data suggest that appropriate fungal antigens and adjuvant can be used to protect against candidal vaginitis, by either route.

  15. Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.

    PubMed

    Le Luduec, Jean-Benoît; Debeer, Sabine; Piras, Fabienne; Andréoni, Christine; Boudet, Florence; Laurent, Philippe; Kaiserlian, Dominique; Dubois, Bertrand

    2016-02-10

    Intradermal (ID) vaccination constitutes a promising approach to induce anti-infectious immunity. This route of immunization has mostly been studied with influenza split-virion vaccines. However, the efficacy of ID vaccination for sub-unit vaccines in relation to underlying skin innate immunity remains to be explored for wider application in humans. Relevant animal models that more closely mimic human skin immunity than the widely used mouse models are therefore necessary. Here, we show in domestic swine, which shares striking anatomic and functional properties with human skin, that a single ID delivery of pseudorabies virus (PRV) glycoproteins without added adjuvant is sufficient to trigger adaptive cellular and humoral immune responses, and to confer protection from a lethal respiratory infection with PRV. Analysis of early events at the skin injection site revealed up-regulation of pro-inflammatory cytokine and chemokine genes, recruitment of neutrophils and monocytes and accumulation of inflammatory DC. We further show that the sustained induction of pro-inflammatory cytokine genes results from the combined effects of skin puncture, liquid injection in the dermis and viral antigens. These data highlight that immune protection against respiratory infection can be induced by ID vaccination with a subunit vaccine and reveal that adjuvant requirements are circumvented by the mechanical and antigenic stress caused by ID injection, which triggers innate immunity and mobilization of inflammatory DC at the immunization site. ID vaccination with sub-unit vaccines may thus represent a safe and efficient solution for protection against respiratory infections in swine and possibly also in humans, given the similarity of skin structure and function in both species.

  16. IFLA General Conference, 1991. Division of Special Libraries Services: Section of Social Science Libraries; Section of Geography and Map Libraries; Section of Biological and Medical Sciences Libraries; Section of Art Libraries. Booklet 2.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    The 10 papers in this booklet were presented at meetings of 4 sections within the Division of Special Libraries: (1) "Information Ensurance of a Scientist" (V. Matveyev, USSR); (2) "Linguistic Barriers and Machine Translation" (Stanley Kalkus, USA); (3) "Maps for Planning" (V. I. Zhukov and L. G. Rudenko, USSR); (4) "Development of Atlas…

  17. TolC plays a crucial role in immune protection conferred by Edwardsiella tarda whole-cell vaccines

    PubMed Central

    Wang, Chao; Peng, Bo; Li, Hui; Peng, Xuan-xian

    2016-01-01

    Although vaccines developed from live organisms have better efficacy than those developed from dead organisms, the mechanisms underlying this differential efficacy remain unexplored. In this study, we combined sub-immunoproteomics with immune challenge to investigate the action of the outer membrane proteome in the immune protection conferred by four Edwardsiella tarda whole-cell vaccines prepared via different treatments and to identify protective immunogens that play a key role in this immune protection. Thirteen spots representing five outer membrane proteins and one cytoplasmic protein were identified, and it was found that their abundance was altered in relation with the immune protective abilities of the four vaccines. Among these proteins, TolC and OmpA were found to be the key immunogens conferring the first and second highest degrees of protection, respectively. TolC was detected in the two effective vaccines (live and inactivated-30-F). The total antiserum and anti-OmpA titers were higher for the two effective vaccines than for the two ineffective vaccines (inactivated-80-F and inactivated-100). Further evidence demonstrated that the live and inactivated-30-F vaccines demonstrated stronger abilities to induce CD8+ and CD4+ T cell differentiation than the other two evaluated vaccines. Our results indicate that the outer membrane proteome changes dramatically following different treatments, which contributes to the effectiveness of whole-cell vaccines. PMID:27406266

  18. Culture Keepers III: Making Global Connections. Proceedings of the Black Caucus of the American Library Association (BCALA) National Conference of African American Librarians (3rd, Winston-Salem, North Carolina, July 31-August 3, 1997).

    ERIC Educational Resources Information Center

    Neely, Teresa Y., Ed.

    The 49 papers presented in this volume are evidence of the research, scholarship, and professional nature of the offerings of the third National Conference of African American Librarians, sponsored by the Black Caucus of the American Library Association. The papers are arranged into nine tracks and Pre-Conference topics: I: Library Connections: An…

  19. White House Conference on Library and Information Services. Hearing before the Subcommittee on Education, Arts and Humanities of the Committee on Labor and Human Resources. United States Senate, One Hundredth Congress, First Session.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. Senate Committee on Labor and Human Resources.

    Held to authorize a White House Conference on Library and Information Services (WHCLIST) in 1989, this hearing focused on library services for literacy, productivity, and democracy as well as on the conference structure and costs. Statements and supporting information provided by the following individuals and institutions were presented: (1)…

  20. Prison Legal Libraries, Idea into Reality; Conference Proceedings (University of California, Berkeley, April 22, 1972).

    ERIC Educational Resources Information Center

    California Univ., Berkeley. School of Librarianship.

    The successful functioning of a democratic form of government depends on a number of very basic principles. The issue confronted at this conference, the provision of legal research materials to prisoners, is tied very closely to two of these principles: first, that no individual should be without recourse to the courts and to the law; and second,…

  1. Brucella abortus ΔrpoE1 confers protective immunity against wild type challenge in a mouse model of brucellosis.

    PubMed

    Willett, Jonathan W; Herrou, Julien; Czyż, Daniel M; Cheng, Jason X; Crosson, Sean

    2016-09-30

    The Brucella abortus general stress response (GSR) system regulates activity of the alternative sigma factor, σ(E1), which controls transcription of approximately 100 genes and is required for persistence in a BALB/c mouse chronic infection model. We evaluated the host response to infection by a B. abortus strain lacking σ(E1) (ΔrpoE1), and identified pathological and immunological features that distinguish ΔrpoE1-infected mice from wild-type (WT), and that correspond with clearance of ΔrpoE1 from the host. ΔrpoE1 infection was indistinguishable from WT in terms of splenic bacterial burden, inflammation and histopathology up to 6weeks post-infection. However, Brucella-specific serum IgG levels in ΔrpoE1-infected mice were 5 times higher than WT by 4weeks post-infection, and remained significantly higher throughout the course of a 12-week infection. Total IgG and Brucella-specific IgG levels peaked strongly in ΔrpoE1-infected mice at 6weeks, which correlated with reduced splenomegaly and bacterial burden relative to WT-infected mice. Given the difference in immune response to infection with wild-type and ΔrpoE1, we tested whether ΔrpoE1 confers protective immunity to wild-type challenge. Mice immunized with ΔrpoE1 completely resisted WT infection and had significantly higher serum titers of Brucella-specific IgG, IgG2a and IFN-γ after WT challenge relative to age-matched naïve mice. We conclude that immunization of BALB/c mice with the B. abortus GSR pathway mutant, ΔrpoE1, elicits an adaptive immune response that confers significant protective immunity against WT infection.

  2. Wide Screening of Phage-Displayed Libraries Identifies Immune Targets in Planta

    PubMed Central

    Rioja, Cristina; Van Wees, Saskia C.; Charlton, Keith A.; Pieterse, Corné M. J.; Lorenzo, Oscar; García-Sánchez, Susana

    2013-01-01

    Microbe-Associated Molecular Patterns and virulence effectors are recognized by plants as a first step to mount a defence response against potential pathogens. This recognition involves a large family of extracellular membrane receptors and other immune proteins located in different sub-cellular compartments. We have used phage-display technology to express and select for Arabidopsis proteins able to bind bacterial pathogens. To rapidly identify microbe-bound phage, we developed a monitoring method based on microarrays. This combined strategy allowed for a genome-wide screening of plant proteins involved in pathogen perception. Two phage libraries for high-throughput selection were constructed from cDNA of plants infected with Pseudomonas aeruginosa PA14, or from combined samples of the virulent isolate DC3000 of Pseudomonas syringae pv. tomato and its avirulent variant avrRpt2. These three pathosystems represent different degrees in the specificity of plant-microbe interactions. Libraries cover up to 2×107 different plant transcripts that can be displayed as functional proteins on the surface of T7 bacteriophage. A number of these were selected in a bio-panning assay for binding to Pseudomonas cells. Among the selected clones we isolated the ethylene response factor ATERF-1, which was able to bind the three bacterial strains in competition assays. ATERF-1 was rapidly exported from the nucleus upon infiltration of either alive or heat-killed Pseudomonas. Moreover, aterf-1 mutants exhibited enhanced susceptibility to infection. These findings suggest that ATERF-1 contains a microbe-recognition domain with a role in plant defence. To identify other putative pathogen-binding proteins on a genome-wide scale, the copy number of selected-vs.-total clones was compared by hybridizing phage cDNAs with Arabidopsis microarrays. Microarray analysis revealed a set of 472 candidates with significant fold change. Within this set defence-related genes, including well

  3. Libraries, Archives and Museums: What's in Them for Us? PIALA '98. Selected Papers from the Pacific Islands Association of Libraries and Archives Conference (8th, Tofol, Kosrae, Federated States of Micronesia, November 17-20, 1998).

    ERIC Educational Resources Information Center

    Cohen, Arlene, Ed.

    This proceedings contains papers from the 1998 annual conference of the Pacific Islands Association of Libraries and Archives (PIALA). After welcoming remarks from Henry Robert and Isabel Rungrad, the following papers are included: "Sharing Our Successes, Discussing Our Future: A Survey of Pacific Collections Activities--Report from the University…

  4. New Beginnings: The Library as an Information and Resource Basket. Selected Papers from PIALA 2008: Pacific Islands Association of Libraries, Archives and Museums Annual Conference (18th, Colonia, Yap, Federated States of Micronesia, November 17-21, 2001)

    ERIC Educational Resources Information Center

    Horie, Ruth H., Ed.

    2009-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. The volume begins with a listing of the members of the PIALA 2008 Planning Committee, the Officers and Executive Board, and Acknowledgements, followed by the Schedule of Events and a…

  5. PIALA '95. Preservation of Culture through Archives and Libraries. Papers from the Annual Pacific Islands Association of Libraries and Archives Conference (5th, Colonia, Yap, Federated States of Micronesia, November 6-10, 1995).

    ERIC Educational Resources Information Center

    Cohen, Arlene, Ed.

    The annual conference of the Pacific Islands Association of Libraries and Archives addressed various topics of interest to librarians, archivists, and educators in the Pacific Islands. The proceedings include welcoming remarks by 2 Yap state government officials and PIALA President Herbert Del Rosario; a keynote address by Dr. Marcia J.…

  6. Marketing: Putting People in the Process. White House Conference on Library and Information Services.

    ERIC Educational Resources Information Center

    Leisner, Anthony

    Noting that some public, school, law, academic, and special libraries have adopted marketing as a management tool, this paper begins by discussing marketing principles as defined by the American Marketing Association (AMA). Librarians are advised to center their marketing activities around what is known as the "4 P's" of marketing: Product, Price,…

  7. Preservation Integrity of Our Nation's Records at Risk. White House Conference on Library and Information Services.

    ERIC Educational Resources Information Center

    Fusonie, Alan

    This paper focuses on the preservation of documents that are disintegrating rapidly in national archives. After providing an introduction and background to the situation, the paper outlines a national preservation strategy for administrators and discusses ways to protect collections in archives and libraries throughout the United States. The…

  8. PIALA '97. Wasahn Kamarain: Place of Enlightenment. Papers from the Pacific Islands Association of Libraries and Archives Conference (7th, Palikir, Pohnpei, November 3-5, 1997).

    ERIC Educational Resources Information Center

    Cohen, Arlene, Ed.

    This PIALA 1997 Proceedings follows the tradition of publishing papers from each annual conference of the Pacific Islands Association of Libraries and Archives (PIALA). After welcoming remarks from Sue Moses, Kapily Capelle, and Isabel Rungrad, the following papers are included: "Places of Enlightenment, Places of Dreams" (Maradel Gale);…

  9. Proceeding of the ACM/IEEE-CS Joint Conference on Digital Libraries (1st, Roanoke, Virginia, June 24-28, 2001).

    ERIC Educational Resources Information Center

    Association for Computing Machinery, New York, NY.

    Papers in this Proceedings of the ACM/IEEE-CS Joint Conference on Digital Libraries (Roanoke, Virginia, June 24-28, 2001) discuss: automatic genre analysis; text categorization; automated name authority control; automatic event generation; linked active content; designing e-books for legal research; metadata harvesting; mapping the…

  10. A National Bibliographic/Resource Sharing Network for Canadian Academic Libraries. Proceedings of the Conference (Ste-Foy, Quebec, October 24-25, 1979).

    ERIC Educational Resources Information Center

    Canadian Association of Research Libraries, Montreal (Quebec).

    These papers discuss various options for a computerized national bibliographic network and highlight the problems and potential of developing such a database. Following a brief explanation of the conference purpose and organization, 10 primary papers are presented: (1) "A Brief Overview of Computerized Library Networking in Canada," by Peter…

  11. IFLA General Conference, 1985. International Association of Law Libraries (IALL) Round-Table on Access to Information in International Legal Research. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on international access to information pertaining to legal research, which were presented at the 1985 conference of the International Federation of Library Associations (IFLA) include: (1) "Materials of European Intergovernmental Organizations and Their Accessibility through Available Research Tools" (Irene Berkey, Northwestern University…

  12. SLA 2002: Putting Knowledge to Work. Papers Presented at the Special Libraries Association Conference (Los Angeles, California, June 9-12, 2002).

    ERIC Educational Resources Information Center

    Special Libraries Association, Washington, DC.

    This document contains the following papers from the 2002 Special Libraries Association Conference: (1) "Competencies for the 21st Century Information Professional: Translating the SLA Competencies into Business Competencies" (Sue Henczel); (2) "Compromises along the Way: Balancing Speed to Market with Sustainability While Delivering Knowledge…

  13. White House Conference on Library and Information Services: Keynote Addresses, Remarks, and Presented Papers (2nd, Washington, D.C., July 9-13, 1991).

    ERIC Educational Resources Information Center

    National Commission on Libraries and Information Science, Washington, DC.

    This collection of addresses, remarks, and papers presented at the second White House Conference on Library and Information Services (WHCLIS2) includes excerpts from remarks made by First Lady Barbara Bush, President George Bush, Marilyn Quayle, and William T. Esrey of United Telecommunications, Inc., and keynote addresses by Mary Futrell from the…

  14. Energies for Transition. Proceedings of the National Conference of the Association of College and Research Libraries (4th, Baltimore, Maryland, April 9-12, 1986).

    ERIC Educational Resources Information Center

    Nitecki, Danuta A., Ed.

    This proceedings of the 1986 conference of the Association of College and Research Libraries contains 60 papers--44 position papers, 7 research reports, and 9 idea briefs--dealing with the issue of transitions facing librarians and ways to respond to these transitions through both formal and informal means. Papers are organized under eight broad…

  15. IFLA General Conference, 1989. Division of Libraries Serving the General Public. Section on Library Services to Multicultural Populations; Round Table--International Association of Metropolitan City Libraries; Round Table on Mobile Libraries. Booklet 31.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The seven papers in this collection continue the presentations from the Division of Libraries Serving the General Public: (1) "Multicultural Activities for Children and Young People at the Munich International Youth Library" (German and English versions; Lioba Betten); (2) "Multicultural Literature and Library Services for Children: A Continuing…

  16. Proceedings of the Binational Conference on Libraries in California and Baja California (1st, Tijuana, Baja California, Mexico, January 13-14, 1984) = Memorias de la Primera Conferencia Binacional de Bibliotecas de las Californias.

    ERIC Educational Resources Information Center

    Ayala, Marta Stiefel, Ed.; And Others

    This document includes the text of presentations given at the First Binational Conference on Libraries in California and Baja California, as well as minutes from four roundtables held at the conference. Following a prologue and a brief background on the conference, the following presentations are included: (1) "State Support for Public Libraries…

  17. Construction of environmental DNA libraries in Escherichia coli and screening for the presence of genes conferring utilization of 4-hydroxybutyrate.

    PubMed

    Henne, A; Daniel, R; Schmitz, R A; Gottschalk, G

    1999-09-01

    Environmental DNA libraries from three different soil samples were constructed. The average insert size was 5 to 8 kb and the percentage of plasmids with inserts was approximately 80%. The recombinant Escherichia coli strains (approximately 930,000) were screened for 4-hydroxybutyrate utilization. Thirty-six positive E. coli clones were obtained during the initial screen, and five of them contained a recombinant plasmid (pAH1 to pAH5) which conferred a stable 4-hydroxybutyrate-positive phenotype. These E. coli clones were studied further. All five were able to grow with 4-hydroxybutyrate as sole carbon and energy source and exhibited 4-hydroxybutyrate dehydrogenase activity in crude extracts. Sequencing of pAH5 revealed a gene homologous to the gbd gene of Ralstonia eutropha, which encodes a 4-hydroxybutyrate dehydrogenase. Two other genes (orf1 and orf6) conferring utilization of 4-hydroxybutyrate were identified during subcloning and sequencing of the inserts of pAH1 and pAH3. The deduced orf1 gene product showed similarities to members of the DedA family of proteins. The sequence of the deduced orf6 gene product harbors the fingerprint pattern of enoyl-coenzyme A hydratases/isomerases. The other sequenced inserts of the plasmids recovered from the positive clones revealed no significant similarity to any other gene or gene product whose sequence is available in the National Center for Biotechnology Information databases.

  18. International Conference of Directors of National Libraries on Resource Sharing in Asia and Oceanic [Proceedings] (Canberra, Australia, May 14-18, 1979). Development of Resource Sharing Networks. Networks Study No. 11.

    ERIC Educational Resources Information Center

    National Library of Australia, Canberra.

    The proceedings of this 1979 conference on library cooperation begin with proposals for the promotion of resource sharing among the national libraries of Asia and Oceania, the text of a policy statement on the role of national and international systems as approved at a 1976 meeting of directors of national libraries held in Lausanne, and a summary…

  19. Chloroplast-derived vaccine antigens confer dual immunity against cholera and malaria by oral or injectable delivery

    PubMed Central

    Davoodi-Semiromi, Abdoreza; Schreiber, Melissa; Nallapali, Samson; Verma, Dheeraj; Singh, Nameirakpam D.; Banks, Robert K.; Chakrabarti, Debopam; Daniell, Henry

    2009-01-01

    Summary Cholera and malaria are major diseases causing high mortality. The only licensed cholera vaccine is expensive; immunity is lost in children within 3 years and adults are not fully protected. No vaccine is yet available for malaria. Therefore, in this study, the cholera toxin-B subunit (CTB) of Vibrio cholerae fused to malarial vaccine antigens apical membrane antigen-1 (AMA1) and merozoite surface protein-1 (MSP1) was expressed in lettuce and tobacco chloroplasts. Southern blot analysis confirmed homoplasmy and stable integration of transgenes. CTB-AMA1 and CTB-MSP1 fusion proteins accumulated up to 13.17% and 10.11% (total soluble protein, TSP) in tobacco and up to 7.3% and 6.1% (TSP) in lettuce respectively. Nine groups of mice (n = 10/group) were immunized subcutaneously (SQV) or orally (ORV) with purified antigens or transplastomic tobacco leaves. Significant levels of antigen-specific antibody titres of immunized mice completely inhibited proliferation of the malarial parasite and cross-reacted with the native parasite proteins in immunoblots and immunofluorescence studies. Protection against cholera toxin challenge in both ORV (100%) and SQV (89%) mice correlated with CTB-specific titres of intestinal, serum IgA and IgG1 in ORV and only IgG1 in SQV mice, but no other immunoglobulin. Increasing numbers of interleukin-10+ T cell but not Foxp3+ regulatory T cells, suppression of interferon-γ and absence of interleukin-17 were observed in protected mice, suggesting that immunity is conferred via the Tr1/Th2 immune response. Dual immunity against two major infectious diseases provided by chloroplast-derived vaccine antigens for long-term (>300 days, 50% of mouse life span) offers a realistic platform for low cost vaccines and insight into mucosal and systemic immunity. PMID:20051036

  20. Thymus cDNA library survey uncovers novel features of immune molecules in Chinese giant salamander Andrias davidianus.

    PubMed

    Zhu, Rong; Chen, Zhong-Yuan; Wang, Jun; Yuan, Jiang-Di; Liao, Xiang-Yong; Gui, Jian-Fang; Zhang, Qi-Ya

    2014-10-01

    A ranavirus-induced thymus cDNA library was constructed from Chinese giant salamander, the largest extant amphibian species. Among the 137 putative immune-related genes derived from this library, these molecules received particular focus: immunoglobulin heavy chains (IgM, IgD, and IgY), IFN-inducible protein 6 (IFI6), and T cell receptor beta chain (TCRβ). Several unusual features were uncovered: IgD displays a structure pattern distinct from those described for other amphibians by having only four constant domains plus a hinge region. A unique IgY form (IgY(ΔFc)), previously undescribed in amphibians, is present in serum. Alternative splicing is observed to generate IgH diversification. IFI6 is newly-identified in amphibians, which occurs in two forms divergent in subcelluar distribution and antiviral activity. TCRβ immunoscope profile follows the typical vertebrate pattern, implying a polyclonal T cell repertoire. Collectively, the pioneering survey of ranavirus-induced thymus cDNA library from Chinese giant salamander reveals immune components and characteristics in this primitive amphibian.

  1. Coadministration of protoxin Cry1Ac from Bacillus thuringiensis with metacestode extract confers protective immunity to murine cysticercosis.

    PubMed

    Ibarra-Moreno, S; García-Hernández, A L; Moreno-Fierros, L

    2014-06-01

    The Bacillus thuringiensis Cry1Ac protoxin (pCry1Ac) is a promising mucosal immunogen and adjuvant that induces protective immunity against Naegleria fowleri and malaria infection models. We determined whether pCry1Ac acted as a protective adjuvant against infection with Taenia crassiceps. BALB/C mice were thrice i.p. immunized with (i) pCry1Ac, (ii) metacestode extract, (iii) extract + pCry1Ac or (iv) vehicle, challenged with metacestodes on day 26 and then sacrificed 35 days later. Cysticerci in the peritoneal cavity were counted, while the serum antibody response and cytokines were analysed after immunization and during infection. Only immunization with pCry1Ac plus extract conferred a significant protection (up to 47%). This group presented fluctuating antibody peaks during infection and the highest IgG1 and IgM titres. Immunization with extract alone elicited high IgG1 and the highest IgG2a responses after 25 days of infection, while nonimmunized mice presented a poor, mixed-Th1/Th2 response during infection. Sharp peaks of TNFα and IFN-γ occurred immediately after the first immunization with extract, especially in the presence of pCry1Ac, but not after the challenge, while in the control and pCry1Ac-alone groups, cytokines were only detected after the challenge. The data support the protective-adjuvant effect of co-administration of pCry1Ac in cysticercosis. PMID:24484070

  2. Coadministration of protoxin Cry1Ac from Bacillus thuringiensis with metacestode extract confers protective immunity to murine cysticercosis.

    PubMed

    Ibarra-Moreno, S; García-Hernández, A L; Moreno-Fierros, L

    2014-06-01

    The Bacillus thuringiensis Cry1Ac protoxin (pCry1Ac) is a promising mucosal immunogen and adjuvant that induces protective immunity against Naegleria fowleri and malaria infection models. We determined whether pCry1Ac acted as a protective adjuvant against infection with Taenia crassiceps. BALB/C mice were thrice i.p. immunized with (i) pCry1Ac, (ii) metacestode extract, (iii) extract + pCry1Ac or (iv) vehicle, challenged with metacestodes on day 26 and then sacrificed 35 days later. Cysticerci in the peritoneal cavity were counted, while the serum antibody response and cytokines were analysed after immunization and during infection. Only immunization with pCry1Ac plus extract conferred a significant protection (up to 47%). This group presented fluctuating antibody peaks during infection and the highest IgG1 and IgM titres. Immunization with extract alone elicited high IgG1 and the highest IgG2a responses after 25 days of infection, while nonimmunized mice presented a poor, mixed-Th1/Th2 response during infection. Sharp peaks of TNFα and IFN-γ occurred immediately after the first immunization with extract, especially in the presence of pCry1Ac, but not after the challenge, while in the control and pCry1Ac-alone groups, cytokines were only detected after the challenge. The data support the protective-adjuvant effect of co-administration of pCry1Ac in cysticercosis.

  3. Rethinking the Library in the Information Age: What Role for Research? [Summary of a Conference/Workshop on Building a Library Research Infrastructure (October 31-November 1, 1988)].

    ERIC Educational Resources Information Center

    Holley, Edward G.; Kenan, William Rand, Jr.

    The question "What is the role for research in library and information science?" lies at the heart of an ongoing project sponsored by the Office of Library Programs in the U.S. Department of Education. The project, which was begun in 1986-87, is designed to identify researchable issues that could help libraries attain or maintain a leadership…

  4. Genetic diversity confers colony-level benefits due to individual immunity.

    PubMed

    Simone-Finstrom, Michael; Walz, Megan; Tarpy, David R

    2016-03-01

    Several costs and benefits arise as a consequence of eusociality and group-living. With increasing group size, spread of disease among nest-mates poses selective pressure on both individual immunity and group-level mechanisms of disease resistance (social immunity). Another factor known to influence colony-level expression of disease is intracolony genetic diversity, which in honeybees (Apis mellifera) is a direct function of the number of mates of the queen. Colonies headed by queens with higher mating numbers have less variable infections of decreased intensity, though the underlying mechanisms remain unclear. By pathogen-challenging larvae in vitro, we decoupled larval immune response from mechanisms of social immunity. Our results show that baseline immunity and degree of immune response do not vary with genetic diversity. However, intracolony variance in antimicrobial peptide production after pathogen challenge decreases with increasing genetic diversity. This reduction in variability of the larval immune response could drive the mitigation of disease observed in genetically diverse colonies. PMID:26961896

  5. 99th Dahlem conference on infection, inflammation and chronic inflammatory disorders: innate immune responses in plants.

    PubMed

    Schulze-Lefert, P

    2010-04-01

    Plants rely exclusively upon mechanisms of innate immunity. Current concepts of the plant innate immune system are based largely on two forms of immunity that engage distinct classes of immune receptors. These receptors enable the recognition of non-self structures that are either conserved between members of a microbial class or specific to individual strains of a microbe. One type of receptor comprises membrane-resident pattern recognition receptors (PRRs) that detect widely conserved microbe-associated molecular patterns (MAMPs) on the cell surface. A second type of mainly intracellular immune sensors, designated resistance (R) proteins, recognizes either the structure or function of strain-specific pathogen effectors that are delivered inside host cells. Phytopathogenic microorganisms have evolved a repertoire of effectors, some of which are delivered into plant cells to sabotage MAMP-triggered immune responses. Plants appear to have also evolved receptors that sense cellular injury by the release and perception of endogenous damage-associated molecular patterns (DAMPs). It is possible that the integration of MAMP and DAMP responses is critical to mount robust MAMP-triggered immunity. This signal integration might help to explain why plants are colonized in nature by remarkably diverse and seemingly asymptomatic microbial communities. PMID:20415853

  6. Selection of bisphenol A - single-chain antibodies from a non-immunized mouse library by ribosome display.

    PubMed

    Zhao, Li; Ning, Baoan; Bai, Jialei; Chen, Xiang; Peng, Yuan; Sun, Siming; Li, Guimin; Fan, Xianjun; Liu, Yuanyuan; Liu, Jianqing; Sun, Yanan; Gao, Zhixian; Zhang, Juankun

    2015-11-01

    Developing reagents with high affinity and specificity are critical to detect the environmental hormones or toxicants. Ribosome display technology has been widely used in functional protein or peptide screening and in directed evolution of protein molecules in vitro. In this study, single-chain variable fragments (scFvs) against bisphenol A (BPA) were selected from a library constructed from splenocytes of non-immunized mice. After five rounds of selection, the selected scFvs bound to BPA with high affinity. Indirect competitive enzyme-linked immunosorbent assay (ELISA) was introduced to screen the antibody affinity and specificity to BPA. The equilibrium dissociation constants (KDS) of one clone was 1.76μM as determined by surface plasmon resonance (SPR). This study indicated that ribosome display can isolate binders to small molecules from a non-immunized naive library without any in vivo steps and can generate recombinant antibodies efficiently and rapidly. In addition, this study provides a methodological framework for detection of small molecules using recombinant antibodies.

  7. Immunization with BLS-Stx2B chimera totally protects dams from early pregnancy loss induced by Shiga toxin type 2 (Stx2) and confers anti-Stx2 immunity to the offspring.

    PubMed

    Sacerdoti, Flavia; Mejías, María P; Bruballa, Andrea C; Alvarez, Romina Soledad; Amaral, María M; Palermo, Marina S; Ibarra, Cristina

    2016-09-01

    Shiga toxin producing Escherichia coli (STEC) are bacterial pathogens involved in food-borne diseases. Shiga toxin (Stx) is the main virulence factor of STEC and is responsible for systemic complications including Hemolytic Uremic Syndrome (HUS). It has been previously demonstrated that Shiga toxin type 2 (Stx2) induces pregnancy loss in rats in early stage of pregnancy. The main purpose of this study was to determine if an active immunization prevents Stx2 mediated pregnancy loss and confers passive protective immunity to the offspring. For that purpose Sprague Dawley female rats were immunized with the chimera based on the enzyme lumazine synthase from Brucella spp. (BLS) and the B subunit of Shiga toxin 2 (Stx2B) named BLS-Stx2B. After immunization females were mated with males. At day 8 of gestation, dams were challenged intraperitoneally with a sublethal and abortifacient dose of Stx2. The immunization induced high anti-Stx2B-specific antibody titers in sera and most important, prevented pregnancy loss. Pups born and breastfeed by immunized dams had high anti-Stx2B-specific antibody titers in sera. Cross-fostering experiments indicated that passive protective immunity against Stx2 was transmitted through lactation. These results indicate that immunization of adult female rats with BLS-Stx2B prevents Stx2-induced pregnancy loss and confers anti Stx2 protective immunity to the offspring.

  8. Immunization with BLS-Stx2B chimera totally protects dams from early pregnancy loss induced by Shiga toxin type 2 (Stx2) and confers anti-Stx2 immunity to the offspring.

    PubMed

    Sacerdoti, Flavia; Mejías, María P; Bruballa, Andrea C; Alvarez, Romina Soledad; Amaral, María M; Palermo, Marina S; Ibarra, Cristina

    2016-09-01

    Shiga toxin producing Escherichia coli (STEC) are bacterial pathogens involved in food-borne diseases. Shiga toxin (Stx) is the main virulence factor of STEC and is responsible for systemic complications including Hemolytic Uremic Syndrome (HUS). It has been previously demonstrated that Shiga toxin type 2 (Stx2) induces pregnancy loss in rats in early stage of pregnancy. The main purpose of this study was to determine if an active immunization prevents Stx2 mediated pregnancy loss and confers passive protective immunity to the offspring. For that purpose Sprague Dawley female rats were immunized with the chimera based on the enzyme lumazine synthase from Brucella spp. (BLS) and the B subunit of Shiga toxin 2 (Stx2B) named BLS-Stx2B. After immunization females were mated with males. At day 8 of gestation, dams were challenged intraperitoneally with a sublethal and abortifacient dose of Stx2. The immunization induced high anti-Stx2B-specific antibody titers in sera and most important, prevented pregnancy loss. Pups born and breastfeed by immunized dams had high anti-Stx2B-specific antibody titers in sera. Cross-fostering experiments indicated that passive protective immunity against Stx2 was transmitted through lactation. These results indicate that immunization of adult female rats with BLS-Stx2B prevents Stx2-induced pregnancy loss and confers anti Stx2 protective immunity to the offspring. PMID:27527816

  9. Proteins of Leishmania (Viannia) shawi confer protection associated with Th1 immune response and memory generation

    PubMed Central

    2012-01-01

    Background Leishmania (Viannia) shawi parasite was first characterized in 1989. Recently the protective effects of soluble leishmanial antigen (SLA) from L. (V.) shawi promastigotes were demonstrated using BALB/c mice, the susceptibility model for this parasite. In order to identify protective fractions, SLA was fractionated by reverse phase HPLC and five antigenic fractions were obtained. Methods F1 fraction was purified from L. (V.) shawi parasite extract by reverse phase HPLC. BALB/c mice were immunized once a week for two consecutive weeks by subcutaneous routes in the rump, using 25 μg of F1. After 1 and 16 weeks of last immunization, groups were challenged in the footpad with L. (V.) shawi promastigotes. After 2 months, those same mice were sacrificed and parasite burden, cellular and humoral immune responses were evaluated. Results The F1 fraction induced a high degree of protection associated with an increase in IFN-γ, a decrease in IL-4, increased cell proliferation and activation of CD8+T lymphocytes. Long-term protection was acquired in F1-immunized mice, associated with increased CD4+ central memory T lymphocytes and activation of both CD4+ and CD8+ T cells. In addition, F1-immunized groups showed an increase in IgG2a levels. Conclusions The inductor capability of antigens to generate memory lymphocytes that can proliferate and secrete beneficial cytokines upon infection could be an important factor in the development of vaccine candidates against American Tegumentary Leishmaniasis. PMID:22463817

  10. Antigen mRNA-transfected, allogeneic fibroblasts loaded with NKT-cell ligand confer antitumor immunity.

    PubMed

    Fujii, Shin-ichiro; Goto, Akira; Shimizu, Kanako

    2009-04-30

    The maturation of dendritic cells (DCs) in situ by danger signals plays a central role in linking innate and adaptive immunity. We previously demonstrated that the activation of invariant natural killer T (iNKT) cells by administration of alpha-galactosylceramide (alpha-GalCer)-loaded tumor cells can act as a cellular adjuvant through the DC maturation. In the current study, we used allogeneic fibroblasts loaded with alpha-GalCer and transfected with antigen-encoding mRNA, thus combining the adjuvant effects of iNKT-cell activation with delivery of antigen to DCs in vivo. We found that these cells produce antigen protein and activate NK and iNKT cells. When injected into major histocompatibility complex (MHC)-mismatched mice, they elicited antigen-specific T-cell responses and provided tumor protection, suggesting that these immune responses depend on host DCs. In addition, antigen-expressing fibroblasts loaded with alpha-GalCer lead to a more potent T-cell response than those expressing NK cell ligands. Thus, glycolipid-loaded, mRNA-transfected allogeneic fibroblasts act as cellular vectors to provide iNKT-cell activation, leading to DC maturation and T-cell immunity. By harnessing the innate immune system and generating an adaptive immune response to a variety of antigens, this unique tool could prove clinically beneficial in the development of immunotherapies against malignant and infectious diseases. PMID:19164596

  11. Transdermal immunization of P. falciparum surface antigen (MSP-119) via elastic liposomes confers robust immunogenicity.

    PubMed

    Tyagi, Rajeev K; Garg, Neeraj K; Dalai, Sarat K; Awasthi, Amit

    2016-04-01

    As transdermal immunization results in poor immunogenicity, which is attributed to poor permeability of antigens through the skin, we believed ultradeformable lipid vesicles (elastic liposome) might address the challenges encountered during transdermal immunization. The elastic liposome, versatile carrier, proves better vehicle for transcutaneous delivery of protein, peptide and nucleic acid antigens. Our recently published article (1) is suggestive of improved immunogenicity of carboxyl-terminal 19 kDa fragment of merozoite surface protein-1 (PfMSP-119) of Plasmodium falciparum when administered subcutaneously via elastic liposomes ( Fig. 1 ). PMID:26810033

  12. Vaccination with lentiviral vector expressing the nfa1 gene confers a protective immune response to mice infected with Naegleria fowleri.

    PubMed

    Kim, Jong-Hyun; Sohn, Hae-Jin; Lee, Jinyoung; Yang, Hee-Jong; Chwae, Yong-Joon; Kim, Kyongmin; Park, Sun; Shin, Ho-Joon

    2013-07-01

    Naegleria fowleri, a pathogenic free-living amoeba, causes fatal primary amoebic meningoencephalitis (PAM) in humans and animals. The nfa1 gene (360 bp), cloned from a cDNA library of N. fowleri, produces a 13.1-kDa recombinant protein which is located on pseudopodia, particularly the food cup structure. The nfa1 gene plays an important role in the pathogenesis of N. fowleri infection. To examine the effect of nfa1 DNA vaccination against N. fowleri infection, we constructed a lentiviral vector (pCDH) expressing the nfa1 gene. For the in vivo mouse study, BALB/c mice were intranasally vaccinated with viral particles of a viral vector expressing the nfa1 gene. To evaluate the effect of vaccination and immune responses of mice, we analyzed the IgG levels (IgG, IgG1, and IgG2a), cytokine induction (interleukin-4 [IL-4] and gamma interferon [IFN-γ]), and survival rates of mice that developed PAM. The levels of both IgG and IgG subclasses (IgG1 and IgG2a) in vaccinated mice were significantly increased. The cytokine analysis showed that vaccinated mice exhibited greater IL-4 and IFN-γ production than the other control groups, suggesting a Th1/Th2 mixed-type immune response. In vaccinated mice, high levels of Nfa1-specific IgG antibodies continued until 12 weeks postvaccination. The mice vaccinated with viral vector expressing the nfa1 gene also exhibited significantly higher survival rates (90%) after challenge with N. fowleri trophozoites. Finally, the nfa1 vaccination effectively induced protective immunity by humoral and cellular immune responses in N. fowleri-infected mice. These results suggest that DNA vaccination using a viral vector may be a potential tool against N. fowleri infection. PMID:23677321

  13. Vaccination with lentiviral vector expressing the nfa1 gene confers a protective immune response to mice infected with Naegleria fowleri.

    PubMed

    Kim, Jong-Hyun; Sohn, Hae-Jin; Lee, Jinyoung; Yang, Hee-Jong; Chwae, Yong-Joon; Kim, Kyongmin; Park, Sun; Shin, Ho-Joon

    2013-07-01

    Naegleria fowleri, a pathogenic free-living amoeba, causes fatal primary amoebic meningoencephalitis (PAM) in humans and animals. The nfa1 gene (360 bp), cloned from a cDNA library of N. fowleri, produces a 13.1-kDa recombinant protein which is located on pseudopodia, particularly the food cup structure. The nfa1 gene plays an important role in the pathogenesis of N. fowleri infection. To examine the effect of nfa1 DNA vaccination against N. fowleri infection, we constructed a lentiviral vector (pCDH) expressing the nfa1 gene. For the in vivo mouse study, BALB/c mice were intranasally vaccinated with viral particles of a viral vector expressing the nfa1 gene. To evaluate the effect of vaccination and immune responses of mice, we analyzed the IgG levels (IgG, IgG1, and IgG2a), cytokine induction (interleukin-4 [IL-4] and gamma interferon [IFN-γ]), and survival rates of mice that developed PAM. The levels of both IgG and IgG subclasses (IgG1 and IgG2a) in vaccinated mice were significantly increased. The cytokine analysis showed that vaccinated mice exhibited greater IL-4 and IFN-γ production than the other control groups, suggesting a Th1/Th2 mixed-type immune response. In vaccinated mice, high levels of Nfa1-specific IgG antibodies continued until 12 weeks postvaccination. The mice vaccinated with viral vector expressing the nfa1 gene also exhibited significantly higher survival rates (90%) after challenge with N. fowleri trophozoites. Finally, the nfa1 vaccination effectively induced protective immunity by humoral and cellular immune responses in N. fowleri-infected mice. These results suggest that DNA vaccination using a viral vector may be a potential tool against N. fowleri infection.

  14. IFLA General Conference, 1991. Division of Serving the General Public: Section of Public Libraries; Section of Children's Libraries; Section on School Libraries; Section of Libraries Serving Disadvantaged Persons; Section of Libraries for the Blind; Section of Library Services to Multicultural Populations; ROTNAC (RT); Mobile Libraries (RT). Booklet 3.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    The 17 papers in this booklet were presented at meetings of 7 sections within the Division of Serving the General Public: (1) "Overcoming Illiteracy and Public Library Social Functions Development" (Slava Matlina, USSR); (2) "Impact of Economic and Political Change upon Public Library Services" (E. S. Ponomaryova, USSR); (3) "The Public Library…

  15. College of Education Libraries and Higher Education. Selected Papers Delivered at DES/ATCDE/LA Joint Conference Held at the Windermere Hydro, Bowness-on-Windermere, Westmorland, 16th to 19th October 1973.

    ERIC Educational Resources Information Center

    Wood, Jean, Ed.

    Seven papers are presented from a conference where librarians and their nonlibrarian colleagues met to discuss the problems facing college libraries in the present period of change in British higher education. Papers discuss the role and responsibilities of the library in relation to conventional and alternative instructional methods and the…

  16. Year of the Oceans: Science of Information Handling. [Proceedings of the] Annual Conference of the International Association of Marine Science Libraries and Information Centers (10th, Woods Hole, Massachusetts, October 2-5, 1984).

    ERIC Educational Resources Information Center

    Grundy, R. L., Ed.; Ford, R. T., Ed.

    International Association of Marine Science Libraries and Information Centers (IAMSLIC) conferences provide a format for libraries and information specialists to discuss common interests and concerns so that services and information can be made available to scientists, administrators, engineers, educators and students in the discipline of marine…

  17. Immunization

    MedlinePlus

    ... a lot worse. Some are even life-threatening. Immunization shots, or vaccinations, are essential. They protect against things like measles, ... B, polio, tetanus, diphtheria, and pertussis (whooping cough). Immunizations are important for adults as well as children. ...

  18. Immunizations

    MedlinePlus

    ... How Can I Help a Friend Who Cuts? Immunizations KidsHealth > For Teens > Immunizations Print A A A ... That Shot? en español Las vacunas Why Are Vaccinations Important? Measles, mumps, and whooping cough may seem ...

  19. State Library Conferences as Professional Development Venues: Unbalanced Support for the AASL-Defined Roles of the School Librarian

    ERIC Educational Resources Information Center

    Moreillon, Judi; Cahill, Maria; McKee, Rebecca

    2012-01-01

    The American Association of School Librarians (AASL) released new guidelines for school library programs in the summer of 2009. Empowering Learners: Guidelines for School Library Programs (AASL 2009a), hereafter referred to as EL, spells out the five roles that school librarians must practice to empower library users. The purpose of this…

  20. Art Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on art libraries, librarianship, and documentation presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "The Tyranny of Distance: Art Libraries in Canada," a description by Mary F. Williamson of Canada's regional art libraries which serve both art students and the general public; (2) "A…

  1. International Federation of Library Associations Annual Conference Papers. Education and Research Division: Library Schools and Other Training Aspects, and Round Table on Library History Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Wagenbreth, Hildegard; And Others

    This group of six papers centers on the development of library schools and the training of library personnel. "The Status of Professional Groups in Libraries and Library Education in the GDR," by Hildegard Wagenbreth and Helmut Kubitschek, East Germany, describes the training programs, apprenticeships, courses, and admission criteria of various…

  2. A Fresh Look at Libraries.

    ERIC Educational Resources Information Center

    Milesko-Pytel, Diana

    1979-01-01

    Describes the activities of the Connecticut Governer's Conference as participants prepare for the White House Conference on Library and Information Services. Explains many of the current services being offered through public and school libraries in Connecticut. (LRA)

  3. Salmonella enterica Serovar Typhimurium Lacking hfq Gene Confers Protective Immunity against Murine Typhoid

    PubMed Central

    Lahiri, Amit; Joy, Omana; Chakravortty, Dipshikha

    2011-01-01

    Salmonella enterica is an important enteric pathogen and its various serovars are involved in causing both systemic and intestinal diseases in humans and domestic animals. The emergence of multidrug-resistant strains of Salmonella leading to increased morbidity and mortality has further complicated its management. Live attenuated vaccines have been proven superior over killed or subunit vaccines due to their ability to induce protective immunity. Of the various strategies used for the generation of live attenuated vaccine strains, focus has gradually shifted towards manipulation of virulence regulator genes. Hfq is a RNA chaperon which mediates the binding of small RNAs to the mRNA and assists in post-transcriptional gene regulation in bacteria. In this study, we evaluated the efficacy of the Salmonella Typhimurium Δhfq strain as a candidate for live oral vaccine in murine model of typhoid fever. Salmonella hfq deletion mutant is highly attenuated in cell culture and animal model implying a significant role of Hfq in bacterial virulence. Oral immunization with the Salmonella hfq deletion mutant efficiently protects mice against subsequent oral challenge with virulent strain of Salmonella Typhimurium. Moreover, protection was induced upon both multiple as well as single dose of immunizations. The vaccine strain appears to be safe for use in pregnant mice and the protection is mediated by the increase in the number of CD4+ T lymphocytes upon vaccination. The levels of serum IgG and secretory-IgA in intestinal washes specific to lipopolysaccharide and outer membrane protein were significantly increased upon vaccination. Furthermore, hfq deletion mutant showed enhanced antigen presentation by dendritic cells compared to the wild type strain. Taken together, the studies in murine immunization model suggest that the Salmonella hfq deletion mutant can be a novel live oral vaccine candidate. PMID:21347426

  4. The bacterial DNA repair protein Mfd confers resistance to the host nitrogen immune response.

    PubMed

    Guillemet, Elisabeth; Leréec, Alain; Tran, Seav-Ly; Royer, Corinne; Barbosa, Isabelle; Sansonetti, Philippe; Lereclus, Didier; Ramarao, Nalini

    2016-01-01

    Production of reactive nitrogen species (NO) is a key step in the immune response following infections. NO induces lesions to bacterial DNA, thus limiting bacterial growth within hosts. Using two pathogenic bacteria, Bacillus cereus and Shigella flexneri, we show that the DNA-repair protein Mfd (Mutation-Frequency-Decline) is required for bacterial resistance to the host-NO-response. In both species, a mutant deficient for mfd does not survive to NO, produced in vitro or by phagocytic cells. In vivo, the ∆mfd mutant is avirulent and unable to survive the NO-stress. Moreover, NO induces DNA-double-strand-breaks and point mutations in the Δmfd mutant. In overall, these observations demonstrate that NO damages bacterial DNA and that Mfd is required to maintain bacterial genomic integrity. This unexpected discovery reveals that Mfd, a typical housekeeping gene, turns out to be a true virulence factor allowing survival and growth of the pathogen in its host, due to its capacity to protect the bacterium against NO, a key molecule of the innate immune defense. As Mfd is widely conserved in the bacterial kingdom, these data highlight a mechanism that may be used by a large spectrum of bacteria to overcome the host immune response and especially the mutagenic properties of NO.

  5. New insight in quantitative analysis of vascular permeability during immune reaction (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Kalchenko, Vyacheslav; Molodij, Guillaume; Kuznetsov, Yuri; Smolyakov, Yuri; Israeli, David; Meglinski, Igor; Harmelin, Alon

    2016-03-01

    The use of fluorescence imaging of vascular permeability becomes a golden standard for assessing the inflammation process during experimental immune response in vivo. The use of the optical fluorescence imaging provides a very useful and simple tool to reach this purpose. The motivation comes from the necessity of a robust and simple quantification and data presentation of inflammation based on a vascular permeability. Changes of the fluorescent intensity, as a function of time is a widely accepted method to assess the vascular permeability during inflammation related to the immune response. In the present study we propose to bring a new dimension by applying a more sophisticated approach to the analysis of vascular reaction by using a quantitative analysis based on methods derived from astronomical observations, in particular by using a space-time Fourier filtering analysis followed by a polynomial orthogonal modes decomposition. We demonstrate that temporal evolution of the fluorescent intensity observed at certain pixels correlates quantitatively to the blood flow circulation at normal conditions. The approach allows to determine the regions of permeability and monitor both the fast kinetics related to the contrast material distribution in the circulatory system and slow kinetics associated with extravasation of the contrast material. Thus, we introduce a simple and convenient method for fast quantitative visualization of the leakage related to the inflammatory (immune) reaction in vivo.

  6. The bacterial DNA repair protein Mfd confers resistance to the host nitrogen immune response.

    PubMed

    Guillemet, Elisabeth; Leréec, Alain; Tran, Seav-Ly; Royer, Corinne; Barbosa, Isabelle; Sansonetti, Philippe; Lereclus, Didier; Ramarao, Nalini

    2016-01-01

    Production of reactive nitrogen species (NO) is a key step in the immune response following infections. NO induces lesions to bacterial DNA, thus limiting bacterial growth within hosts. Using two pathogenic bacteria, Bacillus cereus and Shigella flexneri, we show that the DNA-repair protein Mfd (Mutation-Frequency-Decline) is required for bacterial resistance to the host-NO-response. In both species, a mutant deficient for mfd does not survive to NO, produced in vitro or by phagocytic cells. In vivo, the ∆mfd mutant is avirulent and unable to survive the NO-stress. Moreover, NO induces DNA-double-strand-breaks and point mutations in the Δmfd mutant. In overall, these observations demonstrate that NO damages bacterial DNA and that Mfd is required to maintain bacterial genomic integrity. This unexpected discovery reveals that Mfd, a typical housekeeping gene, turns out to be a true virulence factor allowing survival and growth of the pathogen in its host, due to its capacity to protect the bacterium against NO, a key molecule of the innate immune defense. As Mfd is widely conserved in the bacterial kingdom, these data highlight a mechanism that may be used by a large spectrum of bacteria to overcome the host immune response and especially the mutagenic properties of NO. PMID:27435260

  7. The bacterial DNA repair protein Mfd confers resistance to the host nitrogen immune response

    PubMed Central

    Guillemet, Elisabeth; Leréec, Alain; Tran, Seav-Ly; Royer, Corinne; Barbosa, Isabelle; Sansonetti, Philippe; Lereclus, Didier; Ramarao, Nalini

    2016-01-01

    Production of reactive nitrogen species (NO) is a key step in the immune response following infections. NO induces lesions to bacterial DNA, thus limiting bacterial growth within hosts. Using two pathogenic bacteria, Bacillus cereus and Shigella flexneri, we show that the DNA-repair protein Mfd (Mutation-Frequency-Decline) is required for bacterial resistance to the host-NO-response. In both species, a mutant deficient for mfd does not survive to NO, produced in vitro or by phagocytic cells. In vivo, the ∆mfd mutant is avirulent and unable to survive the NO-stress. Moreover, NO induces DNA-double-strand-breaks and point mutations in the Δmfd mutant. In overall, these observations demonstrate that NO damages bacterial DNA and that Mfd is required to maintain bacterial genomic integrity. This unexpected discovery reveals that Mfd, a typical housekeeping gene, turns out to be a true virulence factor allowing survival and growth of the pathogen in its host, due to its capacity to protect the bacterium against NO, a key molecule of the innate immune defense. As Mfd is widely conserved in the bacterial kingdom, these data highlight a mechanism that may be used by a large spectrum of bacteria to overcome the host immune response and especially the mutagenic properties of NO. PMID:27435260

  8. Construction of a full-length cDNA library of Solen grandis dunker and identification of defense- and immune-related genes

    NASA Astrophysics Data System (ADS)

    Sun, Guohua; Liu, Xiangquan; Ren, Lihua; Yang, Jianmin; Wei, Xiumei; Yang, Jialong

    2013-11-01

    The basic genetic characteristics, important functional genes, and entire transcriptome of Solen grandis Dunker were investigated by constructing a full-length cDNA library with the `switching mechanism at the 5'-end of the RNA transcript' (SMART) technique. Total RNA was isolated from the immune-relevant tissues, gills and hemocytes, using the Trizol reagent, and cDNA fragments were digested with Sfi I before being ligated to the pBluescript II SK* vector. The cDNA library had a titer of 1048 cfu μL-1 and a storage capacity of 1.05×106 cfu. Approximately 98% of the clones in the library were recombinants, and the fragment lengths of insert cDNA ranged from 0.8 kb to 3.0 kb. A total of 2038 expressed sequence tags were successfully sequenced and clustered into 965 unigenes. BLASTN analysis showed that 240 sequences were highly similar to the known genes (E-value < 1e -5; percent identity >80%), accounting for 25% of the total unigenes. According to the Gene Ontology, these unigenes were related to several biological processes, including cell structure, signal transport, protein synthesis, transcription, energy metabolism, and immunity. Fifteen of the identified sequences were related to defense and immunity. The full-length cDNA sequence of HSC70 was obtained. The cDNA library of S. grandis provided a useful resource for future researches of functional genomics related to stress tolerance, immunity, and other physiological activities.

  9. [Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus].

    PubMed

    Dubrovskaia, V V; Ulitin, A B; Laman, A G; Gileva, I P; Bormotov, N I; Il'ichev, A A; Brovko, F A; Shchelkunov, S N; Belanov, E F; Tikunova, N V

    2007-01-01

    A combinatorial immune library of human single-chain antibody fragments (scFv) was constructed on the base of genes encoding variable domains of heavy and light chains of immunoglobulins cloned from the lymphocytes of four vaccinia virus (VACV) vaccinated donors. The size of the library was 3 x 10(7) independent clones. After the library was enriched with the clones producing scFv against recombinant analogue of variola virus surface protein prA30L, a panel of unique antibodies specific to both prA30L and VACV was selected from the library. A plaque reduction neutralization test was performed for all selected antibodies and two antibodies were shown to be able to neutralize plaque formation of VACV in Vero E6 cells monolayer. Binding specificities of these antibodies were confirmed using ELISA and Western blot analysis. To determine the amino acid sequences of neutralizing antibodies their genes were sequenced.

  10. OK-432 synergizes with IFN-γ to confer dendritic cells with enhanced antitumor immunity.

    PubMed

    Pan, Ke; Lv, Lin; Zheng, Hai-xia; Zhao, Jing-jing; Pan, Qiu-zhong; Li, Jian-jun; Weng, De-sheng; Wang, Dan-dan; Jiang, Shan-shan; Chang, Alfred E; Li, Qiao; Xia, Jian-chuan

    2014-03-01

    Generation of functional dendritic cells (DCs) with boosted immunity after the withdrawal of initial activation/maturation conditions remains a significant challenge. In this study, we investigated the impact of a newly developed maturation cocktail consisting of OK-432 and interferon-gamma (IFN-γ) on the function of human monocyte-derived DCs (MoDCs). We found that OK-432 plus IFN-γ stimulation could induce significantly stronger expression of surface molecules, production of cytokines, as well as migration of DCs compared with OK-432 stimulation alone. Most importantly, DCs matured with OK-432 plus IFN-γ-induced maintained secretion of interleukin-12 (IL-12)p70 in secondary culture after stimulus withdrawal. Functionally, OK-432 plus IFN-γ-conditioned DCs induce remarkable Th1 and Tc1 responses more effectively than OK-432 alone, even more than the use of α-type-1 cytokine cocktail. As a result, DCs matured with OK-432 plus IFN-γ can prime stronger cytotoxic lymphocyte (CTL) and natural killer (NK) cell response against tumor cells in vitro. Peripheral blood mononuclear cells activated by DCs matured with OK-432 plus IFN-γ also showed greater tumor growth inhibition in vivo in null mice. Molecular mechanistic analysis showed that DC maturation using IFN-γ in concert with OK-432 involves the activation of p38 and nuclear factor-kappa B (NF-κB) pathways. This study provided a novel strategy to generate more potent immune segments in DC vaccine.

  11. It Can Be Done: With Advocacy, Libraries Can Thrive on Pacific Islands. Selected Papers from PIALA 2007, Pacific Islands Association of Libraries, Archives and Museums Annual Conference (17th, Tinian and Saipan, Commonwealth of the Northern Mariana Islands, November 12-17, 2007)

    ERIC Educational Resources Information Center

    Horie, Ruth H., Ed.

    2010-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. The volume begins with a listing of the members of the PIALA 2007 Planning Committee, the Officers and Executive Board, and Acknowledgements, followed by the Pre-Conference Workshop…

  12. Libraries, Archives, and Museums Helping Create Futures: Building on Culture, Knowledge, and Information through Collaboration and Resource Sharing. Selected Papers from PIALA 2010, Pacific Islands Association of Libraries, Archives, and Museums Annual Conference (20th, Weno, Chuuk State, Federated States of Micronesia, November 15-19, 2010)

    ERIC Educational Resources Information Center

    Drake, Paul B., Ed.

    2012-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 20th annual conference was held in Weno, Chuuk State, Federated States of Micronesia, November 15-19, 2010. The volume begins with a listing of the members of the PIALA 2010…

  13. Public Libraries and Community-Based Education: Making the Connection for Lifelong Learning. [Volume 1.] Summary of the Proceedings of a Conference Sponsored by the National Institute on Postsecondary Education, Libraries, and Lifelong Learning (Washington, D.C., April 12-13, 1995).

    ERIC Educational Resources Information Center

    Humes, Barbara A.; And Others

    This document summarizes the activities of a 2-day conference (April 12-13, 1995) during which educators heard eight papers and discussed federal research priorities in the areas of libraries, community-based education, and lifelong learning. Libraries, museums, agricultural extension services, religious organizations, health care organizations,…

  14. Public Libraries and Community-Based Education: Making the Connection for Lifelong Learning. Volume 2: Commissioned Papers. A Conference Sponsored by the National Institute on Postsecondary Education, Libraries, and Lifelong Learning, Office of Educational Research and Improvement (Washington, D.C., April 12-13, 1995).

    ERIC Educational Resources Information Center

    National Inst. on Postsecondary Education, Libraries, and Lifelong Learning (ED/OERI), Washington, DC.

    This conference explored the relationship between the public library, community-based adult education, and lifelong learning. The eight commissioned papers presented include: "Community Based Adult Jewish Learning Program: Issues and Concerns" (Paul A. Flexner); "Rural and Small Libraries: Provisions for Lifelong Learning" (Bernard Vavrek);…

  15. A Report on the 30th Annual Conference of the International Association of Scientific and Technological University Libraries (IATUL)

    ERIC Educational Resources Information Center

    Tripathi, Manorama

    2010-01-01

    Purpose: The paper aims to report on the 30th IATUL Annual Conference held in Leuven, Belgium, 1-4 June, 2009. Design/methodology/approach: The paper summarises the major themes of the conference as well as giving some specific details of developments at the Indira Gandhi National Open University in India, which supplement the author's…

  16. Military Librarians Workshop; Department of Defense Libraries in Transition (14th, 30 November - 2 December 1970). Conference Proceedings.

    ERIC Educational Resources Information Center

    Industrial Coll. of the Armed Forces (DOD), Washington, DC.

    The theme of the 14th Annual Military Librarians Workshop is: "Department of Defense Libraries in Transition." The National War College and the Industrial College libraries seek to see what support they can give each other. The ten workshops are: (1) Standardization of Bibliographic Data, (2) Evolution of Technical Reports, (3) DOD Coordination of…

  17. Libraries: Aids to Life Satisfaction for Older Women. A 1981 White House Conference on the Aging Background Paper.

    ERIC Educational Resources Information Center

    Mathews, Virginia

    This report discusses the ability of public libraries to address the problems of older Americans, particularly older women, and to make their lives more satisfying. A review of facts and figures about the older population is followed by a discussion of programs and responses to the needs of older Americans by libraries and other agencies, such as…

  18. IFLA General Conference, 1985. UNESCO/IFLA Presession Seminar. Public Library Service for All: How to Serve Rural Areas. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on public library service to rural areas, which were presented at the 1985 UNESCO/IFLA (United Nations Educational, Scientific, and Cultural Organization/International Federation of Library Associations) presession seminar are compiled here and include: (1) "Characteristics and Needs of Various Groups, Families, and Other Community Groups"…

  19. DOCK2 confers immunity and intestinal colonization resistance to Citrobacter rodentium infection

    PubMed Central

    Liu, Zhiping; Man, Si Ming; Zhu, Qifan; Vogel, Peter; Frase, Sharon; Fukui, Yoshinori; Kanneganti, Thirumala-Devi

    2016-01-01

    Food poisoning is one of the leading causes of morbidity and mortality in the world. Citrobacter rodentium is an enteric pathogen which attaches itself to enterocytes and induces attachment and effacing (A/E) lesions. The ability of the bacterium to cause infection requires subversion of the host actin cytoskeleton. Rac-dependent actin polymerization is activated by a guanine nucleotide exchange factor known as Dedicator of cytokinesis 2 (DOCK2). However, the role of DOCK2 in infectious disease is largely unexplored. Here, we found that mice lacking DOCK2 were susceptible to C. rodentium infection. These mice harbored increased levels of C. rodentium bacteria, showed more pronounced weight loss and inflammation-associated pathology, and were prone to bacterial dissemination to the systemic organs compared with wild-type mice. We found that mice lacking DOCK2 were more susceptible to C. rodentium attachment to intestinal epithelial cells. Therefore, our results underscored an important role of DOCK2 for gastrointestinal immunity to C. rodentium infection. PMID:27291827

  20. Recombinant Toxoplasma gondii phosphoglycerate mutase 2 confers protective immunity against toxoplasmosis in BALB/c mice

    PubMed Central

    Wang, Hai-Long; Wen, Li-Min; Pei, Yan-Jiang; Wang, Fen; Yin, Li-Tian; Bai, Ji-Zhong; Guo, Rui; Wang, Chun-Fang; Yin, Guo-Rong

    2016-01-01

    Toxoplasmosis is one of the most widespread zoonoses worldwide. It has a high incidence and can result in severe disease in humans and livestock. Effective vaccines are needed to limit and prevent infection with Toxoplasma gondii. In this study, we evaluated the immuno-protective efficacy of a recombinant Toxoplasma gondii phosphoglycerate mutase 2 (rTgPGAM 2) against T. gondii infection in BALB/c mice. We report that the mice nasally immunised with rTgPGAM 2 displayed significantly higher levels of special IgG antibodies against rTgPGAM 2 (including IgG1, IgG2a and IgAs) and cytokines (including IFN-γ, IL-2 and IL-4) in their blood sera and supernatant of cultured spleen cells compared to those of control animals. In addition, an increased number of spleen lymphocytes and enhanced lymphocyte proliferative responses were observed in the rTgPGAM 2-immunised mice. After chronic infection and lethal challenge with the highly virulent T. gondii RH strain by oral gavage, the survival time of the rTgPGAM 2-immunised mice was longer (P < 0.01) and the survival rate (70%) was higher compared with the control mice (P < 0.01). The reduction rate of brain and liver tachyzoites in rTgPGAM 2-vaccinated mice reached approximately 57% and 69% compared with those of the control mice (P < 0.01). These results suggest that rTgPGAM 2 can generate protective immunity against T. gondii infection in BALB/c mice and may be a promising antigen in the further development of an effective vaccine against T. gondii infection. PMID:26984115

  1. Immunization with Fasciola hepatica thioredoxin glutathione reductase failed to confer protection against fasciolosis in cattle.

    PubMed

    Maggioli, Gabriela; Bottini, Gualberto; Basika, Tatiana; Alonzo, Pablo; Salinas, Gustavo; Carmona, Carlos

    2016-07-15

    The liver fluke Fasciola hepatica remains an important agent of food-borne trematode disease producing great economic losses due to its negative effect on productivity of livestock grazing in temperate areas. The prevailing control strategy based on anthelmintic drugs is unsustainable due to widespread resistance hence vaccination appears as an attractive option to pursue. In this study we evaluate the effect of vaccination in calves with a functional recombinant thioredoxin glutathione reductase (rFhTGR) from liver fluke, a critical antioxidant enzyme at the crossroads of the thioredoxin and glutathione metabolism in flatworms. The recombinant enzyme produced in Escherichia coli was tested in two vaccination experiments; in the first trial rFhTGR was administered in combination with Freund́s Incomplete Adjuvant (FIA) in a three-inoculation scheme on weeks 0, 4 and 8; in the second trial rFhTGR was given mixed with Adyuvac 50 or Alum as adjuvants on weeks 0 and 4. In both cases calves were challenged with metacercariae (400 in the first and 500 in the second trial) 2 weeks after the last inoculation. Our results demonstrate that two or three doses of the vaccine induced a non-significant reduction in worm counts of 8.2% (FIA), 3.8% (Adyuvac 50) and 23.0% (Alum) compared to adjuvant controls indicating that rFhTGR failed to induce a protective immunity in challenged calves. All vaccine formulations induced a mixed IgG1/IgG2 response but no booster was observed after challenge. No correlations between antibody titres and worm burdens were found. PMID:27270384

  2. Immunization with Fasciola hepatica thioredoxin glutathione reductase failed to confer protection against fasciolosis in cattle.

    PubMed

    Maggioli, Gabriela; Bottini, Gualberto; Basika, Tatiana; Alonzo, Pablo; Salinas, Gustavo; Carmona, Carlos

    2016-07-15

    The liver fluke Fasciola hepatica remains an important agent of food-borne trematode disease producing great economic losses due to its negative effect on productivity of livestock grazing in temperate areas. The prevailing control strategy based on anthelmintic drugs is unsustainable due to widespread resistance hence vaccination appears as an attractive option to pursue. In this study we evaluate the effect of vaccination in calves with a functional recombinant thioredoxin glutathione reductase (rFhTGR) from liver fluke, a critical antioxidant enzyme at the crossroads of the thioredoxin and glutathione metabolism in flatworms. The recombinant enzyme produced in Escherichia coli was tested in two vaccination experiments; in the first trial rFhTGR was administered in combination with Freund́s Incomplete Adjuvant (FIA) in a three-inoculation scheme on weeks 0, 4 and 8; in the second trial rFhTGR was given mixed with Adyuvac 50 or Alum as adjuvants on weeks 0 and 4. In both cases calves were challenged with metacercariae (400 in the first and 500 in the second trial) 2 weeks after the last inoculation. Our results demonstrate that two or three doses of the vaccine induced a non-significant reduction in worm counts of 8.2% (FIA), 3.8% (Adyuvac 50) and 23.0% (Alum) compared to adjuvant controls indicating that rFhTGR failed to induce a protective immunity in challenged calves. All vaccine formulations induced a mixed IgG1/IgG2 response but no booster was observed after challenge. No correlations between antibody titres and worm burdens were found.

  3. IFLA General Conference, 1990. Division of Libraries Serving the General Public: Section on Public Libraries; Section of Children's Libraries, Children's Literature Documentation Centres, Round Table; Section of School Libraries; Section of Libraries for the Blind; INTAMEL, Round Table; Mobile Libraries, Round Table. Booklet 3.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 28 papers in this collection were presented at meetings of four sections and three round tables within the Division of Libraries Serving the General Public: (1) "Rural Community Information Services: Guidelines for Researching Need, Setting Up Services and Evaluating Performance" (Elaine Kempson); (2) "Library Activities at the Workplace"…

  4. EPIVAC International Conference on Financial Sustainability of Immunization Programs in sub-Saharan Africa, February 16-18, 2012, Ouidah, Benin.

    PubMed

    Drach, Marcel; Le Gargasson, Jean-Bernard; Mathonnat, Jacky; Da Silva, Alfred; Kaddar, Miloud; Colombini, Anaïs

    2013-09-23

    The introduction of new vaccines with much higher prices than traditional vaccines results in increasing budgetary pressure on immunization programs in GAVI-eligible countries, increasing the need to ensure their financial sustainability. In this context, the third EPIVAC (Epidemiology and Vaccinology) technical conference was held from February 16 to 18, 2012 at the Regional Institute of Public Health in Ouidah, Benin. Managers of ministries of health and finance from 11 West African countries (GAVI eligible countries), as well as former EPIVAC students and European experts, shared their knowledge and best practices on immunization financing at district and country level. The conference concluded by stressing five major priorities for the financial sustainability of national immunization programs (NIPs) in GAVI-eligible countries. - Strengthen public financing by increasing resources and fiscal space, improving budget processes, increasing contribution of local governments and strengthen efficiency of budget spending. - Promote equitable community financing which was recognized as a significant and essential contribution to the continuity of EPI operations. - Widen private funding by exploring prospects offered by sponsorship through foundations dedicated to immunization and by corporate social responsibility programs. - Contain the potential crowding-out effect of GAVI co-financing and ensure that decisions on new vaccine introductions are evidence-based. - Seek out innovative financing mechanisms such as taxes on food products or a national solidarity fund. PMID:23892101

  5. Tomato I2 Immune Receptor Can Be Engineered to Confer Partial Resistance to the Oomycete Phytophthora infestans in Addition to the Fungus Fusarium oxysporum.

    PubMed

    Giannakopoulou, Artemis; Steele, John F C; Segretin, Maria Eugenia; Bozkurt, Tolga O; Zhou, Ji; Robatzek, Silke; Banfield, Mark J; Pais, Marina; Kamoun, Sophien

    2015-12-01

    Plants and animals rely on immune receptors, known as nucleotide-binding domain and leucine-rich repeat (NLR)-containing proteins, to defend against invading pathogens and activate immune responses. How NLR receptors respond to pathogens is inadequately understood. We previously reported single-residue mutations that expand the response of the potato immune receptor R3a to AVR3a(EM), a stealthy effector from the late blight oomycete pathogen Phytophthora infestans. I2, another NLR that mediates resistance to the will-causing fungus Fusarium oxysporum f. sp. lycopersici, is the tomato ortholog of R3a. We transferred previously identified R3a mutations to I2 to assess the degree to which the resulting I2 mutants have an altered response. We discovered that wild-type I2 protein responds weakly to AVR3a. One mutant in the N-terminal coiled-coil domain, I2(I141N), appeared sensitized and displayed markedly increased response to AVR3a. Remarkably, I2(I141N) conferred partial resistance to P. infestans. Further, I2(I141N) has an expanded response spectrum to F. oxysporum f. sp. lycopersici effectors compared with the wild-type I2 protein. Our results suggest that synthetic immune receptors can be engineered to confer resistance to phylogenetically divergent pathogens and indicate that knowledge gathered for one NLR could be exploited to improve NLR from other plant species.

  6. Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library.

    PubMed

    Rahumatullah, Anizah; Ahmad, Azimah; Noordin, Rahmah; Lim, Theam Soon

    2015-10-01

    Phage display technology is an important tool for antibody generation or selection. This study describes the development of a scFv library and the subsequent analysis of identified monoclonal antibodies against BmSXP, a recombinant antigen for lymphatic filariasis. The immune library was generated from blood of lymphatic filariasis infected individuals. A TA based intermediary cloning approach was used to increase cloning efficiency for the library construction process. A diverse immune scFv library of 10(8) was generated. Six unique monoclonal antibodies were identified from the 50 isolated clones against BmSXP. Analysis of the clones showed a bias for the IgHV3 and Vκ1 (45.5%) and IgHV2 and Vκ3 (27.3%) gene family. The most favored J segment for light chain is IgKJ1 (45.5%). The most favored D and J segment for heavy chain are IgHD6-13 (75%) and IgHJ3 (47.7%). The information may suggest a predisposition of certain V genes in antibody responses against lymphatic filariasis.

  7. A single immunization with recombinant rabies virus (ERAG3G) confers complete protection against rabies in mice

    PubMed Central

    2014-01-01

    Purpose New alternative bait rabies vaccines applicable to pet dogs and wild animals are needed to eradicate rabies in Korea. In this study, recombinant rabies virus, ERAG3G strain was constructed using reverse genetic system and the safety, efficacy and immunogenicity of the ERAG3G strain was evaluated in mice and dogs. Materials and Methods Using the full-length genome mutated amino acid at position 333 of glycoprotein of rabies virus (RABV) and helper plasmids, the ERAG3G strain was rescued in BHK/T7-9 cells successfully. Mice were inoculated with the ERAG3G strain for safety and efficacy. Safety and immunogenicity of the dog inoculated with the ERAG3G strain (1 mL, 108.0 FAID50/mL) via intramuscular route was evaluated for 28 days after inoculation. Results The ERAG3G strain rescued by reverse genetic system was propagated well in the mouse neuroblastoma cells revealing titer of 108.5 FAID50/mL and was not pathogenic to 4- or 6-week-old mice that received by intramuscular or intracranical route. Immunization with the ERAG3G strain conferred complete protection from lethal RABV in mice. Dogs inoculated with the vaccine candidate via intramuscular route showed high neutralizing antibody titer ranging from 2.62 to 23.9 IU/mL at 28 days postinoculation. Conclusion Our findings suggest that the ERAG3G strain plays an important role in inducing protective efficacy in mice and causes to arise anti-rabies neutralizing antibody in dogs. PMID:25003091

  8. IFLA General Conference, 1992. Division of Management and Technology: Audiovisual Media (RT); Section on Library Services to Multicultural Populations; Section on Library Buildings and Equipment; Section on Information Technology; Management of Library Associations (RT); Section on Statistics. Papers

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    Eleven papers delivered at the annual meeting of the International Federation of Library Associations and Institutions for the Division of Management and Technology are presented. Some were presented at a roundtable on audiovisual media, and others are from sessions on library buildings and equipment, information management, and statistics in…

  9. Visions--the Role of the Library and the Librarian in the Information Age. Proceedings of the Annual Conference, Alabama Library Association, College, University, and Special Libraries Division (Orange Beach, AL, April 28, 1994).

    ERIC Educational Resources Information Center

    Hubbard, William J., Ed.; And Others

    1994-01-01

    This proceedings contains the introduction, keynote address, panel discussion, and four papers. Topics include: role of the library and librarians in the information age, the librarian as information consultant, marketing the library to the parent organization, ownership versus access, and financial realities of providing information services in…

  10. Immunization.

    ERIC Educational Resources Information Center

    Guerin, Nicole; And Others

    1986-01-01

    Contents of this double journal issue concern immunization and primary health care of children. The issue decribes vaccine storage and sterilization techniques, giving particular emphasis to the role of the cold chain, i.e., the maintenance of a specific temperature range to assure potency of vaccines as they are moved from a national storage…

  11. Libraries are for People! But Are the People for Libraries?

    ERIC Educational Resources Information Center

    Ohio State Library, Columbus.

    The 1974 Ohio Governor's Conference on Library and Information Services was the first in a series of meetings mandated by the Long Range Program for the Improvement of Library Services developed by the State Library Board to give citizens a voice in the future of their libraries. Information was gathered on the kinds of libraries--school,…

  12. Emerging Frameworks and Methods. Proceedings of the International Conference on Conceptions of Library and Information Science (CoLIS4) (4th, Seattle, Washington, July 21-25, 2002).

    ERIC Educational Resources Information Center

    Bruce, Harry, Ed.; Fidel, Raya, Ed.; Ingwersen, Peter, Ed.; Vakkari, Pertti, Ed.

    These proceedings are the fourth in the series of international conferences whose general aim is to provide a broad forum for critically exploring and analyzing library and information science as a discipline and as a field of research from historical, theoretical, philosophical, and empirical perspectives. The papers in this volume cover a wide…

  13. PIALA '96. Jaketo Jaketak Kobban Alele Eo--Identifying, Using and Sharing Local Resources. Proceedings of the Annual Pacific Islands Association of Libraries and Archives Conference (6th, Majuro, Marshall Islands, November 5-8, 1996).

    ERIC Educational Resources Information Center

    Cohen, Arlene, Ed.

    This 1996 PIALA conference explores ways to identify and make available local resources on the Marshall Islands. The traditional Marshallese word, "Alele," which means "the basket which holds the tools, treasures and resources needed for everyday life," is also the name of Majuro's public library, museum and Marshall Islands Depository and is…

  14. Intranasal immunization with fusion protein MrpH·FimH and MPL adjuvant confers protection against urinary tract infections caused by uropathogenic Escherichia coli and Proteus mirabilis.

    PubMed

    Habibi, Mehri; Asadi Karam, Mohammad Reza; Shokrgozar, Mohammad Ali; Oloomi, Mana; Jafari, Anis; Bouzari, Saeid

    2015-04-01

    Urinary tract infections (UTIs) caused by Uropathogenic Escherichia coli (UPEC) and Proteus mirabilis are among the most common infections in the world. Currently there are no vaccines available to confer protection against UTI in humans. In this study, the immune responses and protection of FimH of UPEC with MrpH antigen of P. mirabilis in different vaccine formulations with and without MPL adjuvant were assessed. Mice intranasally immunized with the novel fusion protein MrpH·FimH induced a significant increase in IgG and IgA in serum, nasal wash, vaginal wash, and urine samples. Mice immunized with fusion MrpH·FimH also showed a significant boost in cellular immunity. Addition of MPL as the adjuvant enhanced FimH and MrpH specific humoral and cellular responses in both systemic and mucosal samples. Vaccination with MrpH·FimH alone or in combination with MPL showed the highest efficiency in clearing bladder and kidney infections in mice challenged with UPEC and P. mirabilis. These findings may indicate that the protection observed correlates with the systemic, mucosal and cellular immune responses induced by vaccination with these preparations. Our data suggest MrpH·FimH fusion protein with or without MPL as adjuvant could be potential vaccine candidates for elimination of UPEC and P. mirabilis. These data altogether are promising and these formulations are good candidates for elimination of UPEC and P. mirabilis.

  15. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis.

    PubMed

    Silva, Everton F; Medeiros, Marco A; McBride, Alan J A; Matsunaga, Jim; Esteves, Gabriela S; Ramos, João G R; Santos, Cleiton S; Croda, Júlio; Homma, Akira; Dellagostin, Odir A; Haake, David A; Reis, Mitermayer G; Ko, Albert I

    2007-08-14

    Subunit vaccines are a potential intervention strategy against leptospirosis, which is a major public health problem in developing countries and a veterinary disease in livestock and companion animals worldwide. Leptospiral immunoglobulin-like (Lig) proteins are a family of surface-exposed determinants that have Ig-like repeat domains found in virulence factors such as intimin and invasin. We expressed fragments of the repeat domain regions of LigA and LigB from Leptospira interrogans serovar Copenhageni. Immunization of Golden Syrian hamsters with Lig fragments in Freund's adjuvant induced robust antibody responses against recombinant protein and native protein, as detected by ELISA and immunoblot, respectively. A single fragment, LigANI, which corresponds to the six carboxy-terminal Ig-like repeat domains of the LigA molecule, conferred immunoprotection against mortality (67-100%, P<0.05) in hamsters which received a lethal inoculum of L. interrogans serovar Copenhageni. However, immunization with this fragment did not confer sterilizing immunity. These findings indicate that the carboxy-terminal portion of LigA is an immunoprotective domain and may serve as a vaccine candidate for human and veterinary leptospirosis. PMID:17629368

  16. Construction and Screening of Metagenomic Libraries Derived from Enrichment Cultures: Generation of a Gene Bank for Genes Conferring Alcohol Oxidoreductase Activity on Escherichia coli

    PubMed Central

    Knietsch, Anja; Waschkowitz, Tanja; Bowien, Susanne; Henne, Anke; Daniel, Rolf

    2003-01-01

    Enrichment of microorganisms with special traits and the construction of metagenomic libraries by direct cloning of environmental DNA have great potential for identifying genes and gene products for biotechnological purposes. We have combined these techniques to isolate novel genes conferring oxidation of short-chain (C2 to C4) polyols or reduction of the corresponding carbonyls. In order to favor the growth of microorganisms containing the targeted genes, samples collected from four different environments were incubated in the presence of glycerol and 1,2-propanediol. Subsequently, the DNA was extracted from the four samples and used to construct complex plasmid libraries. Approximately 100,000 Escherichia coli strains of each library per test substrate were screened for the production of carbonyls from polyols on indicator agar. Twenty-four positive E. coli clones were obtained during the initial screen. Sixteen of them contained a plasmid (pAK101 to pAK116) which conferred a stable carbonyl-forming phenotype. Eight of the positive clones exhibited NAD(H)-dependent alcohol oxidoreductase activity with polyols or carbonyls as the substrates in crude extracts. Sequencing revealed that the inserts of pAK101 to pAK116 encoded 36 complete and 17 incomplete presumptive protein-encoding genes. Fifty of these genes showed similarity to sequenced genes from a broad collection of different microorganisms. The genes responsible for the carbonyl formation of E. coli were identified for nine of the plasmids (pAK101, pAK102, pAK105, pAK107 to pAK110, pAK115, and pAK116). Analyses of the amino acid sequences deduced from these genes revealed that three (orf12, orf14, and orf22) encoded novel alcohol dehydrogenases of different types, four (orf5, sucB, fdhD, and yabF) encoded novel putative oxidoreductases belonging to groups distinct from alcohol dehydrogenases, one (glpK) encoded a putative glycerol kinase, and one (orf1) encoded a protein which showed no similarity to any

  17. Construction of cDNA library from intestine, mesentery and coelomocyte of Apostichopus japonicus Selenka infected with Vibrio sp. and a preliminary analysis of immunity-related genes

    NASA Astrophysics Data System (ADS)

    Liu, Hongzhan; Zheng, Fengrong; Sun, Xiuqin; Cai, Yimei

    2012-06-01

    The aquaculture of sea cucumber Apostichopus japonicus (Echinodermata, Holothuroidea) has grown rapidly during recent years and has become an important sector of the marine industry in Northern China. However, with the rapid growth of the industry and the use of non-standard culture techniques, epidemic diseases of A. japonicus now pose increasing problems to the industry. To screen the genes with stress response to bacterial infection in sea cucumber at a genome wide level, we constructed a cDNA library from A. japonicus Selenka (Aspidochirotida: Stichopodidae) after infecting them with Vibrio sp. for 48 h. Total RNA was extracted from the intestine, mesentery and coelomocyte of infected sea cucumber using Trizol and mRNA was isolated by Oligotex mRNA Kits. The ligated cDNAs were transformed into DH5α, and a library of 3.24×105 clones (3.24×105 cfu mL-1) was obtained with the sizes of inserted fragments ranging from 0.8 to 2.5 kb. Sequencing the cDNA clones resulted in a total of 1106 ESTs that passed the quality control. BlastX and BlastN searches have identified 168 (31.5%) ESTs sharing significant homology with known sequences in NCBI protein or nucleotide databases. Among a panel of 25 putative immunity-related genes, serum lectin isoform, complement component 3, complement component 3-like genes were further studied by real-time PCR and they all increased more than 5 fold in response to Vibrio sp. challenge. Our library provides a valuable molecular tool for future study of invertebrate immunity against bacterial infection and our gene expression data indicates the importance of the immune system in the evolution and development of sea cucumber.

  18. Public Access to Digital Material; A Call to Researchers: Digital Libraries Need Collaboration across Disciplines; Greenstone: Open-Source Digital Library Software; Retrieval Issues for the Colorado Digitization Project's Heritage Database; Report on the 5th European Conference on Digital Libraries, ECDL 2001; Report on the First Joint Conference on Digital Libraries.

    ERIC Educational Resources Information Center

    Kahle, Brewster; Prelinger, Rick; Jackson, Mary E.; Boyack, Kevin W.; Wylie, Brian N.; Davidson, George S.; Witten, Ian H.; Bainbridge, David; Boddie, Stefan J.; Garrison, William A.; Cunningham, Sally Jo; Borgman, Christine L.; Hessel, Heather

    2001-01-01

    These six articles discuss various issues relating to digital libraries. Highlights include public access to digital materials; intellectual property concerns; the need for collaboration across disciplines; Greenstone software for construction and presentation of digital information collections; the Colorado Digitization Project; and conferences…

  19. IFLA General Conference, 1991. Division of Education and Research: Section of Education and Training; Continuing Professional Education (RT); Section of Library Theory and Research; Library History (RT); Research in Reading (RT); Editors of Library Journals (RT). Booklet 7.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    The 12 papers in this collection were presented at 6 sections of the Division of Education and Research: (1) "Emergence of the Asian Pacific Area and Its Impact on Education and Training of Librarians" (Maxine K. Rochester, Australia); (2) "The Cultural Aspects of Colonialism: Case Study of the Relevance of the Imported Curriculum for Library and…

  20. Mutation of the Enterohemorrhagic Escherichia coli Core LPS Biosynthesis Enzyme RfaD Confers Hypersusceptibility to Host Intestinal Innate Immunity In vivo.

    PubMed

    Kuo, Cheng-Ju; Chen, Jenn-Wei; Chiu, Hao-Chieh; Teng, Ching-Hao; Hsu, Tai-I; Lu, Pei-Jung; Syu, Wan-Jr; Wang, Sin-Tian; Chou, Ting-Chen; Chen, Chang-Shi

    2016-01-01

    Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important foodborne pathogen causing severe diseases in humans worldwide. Currently, there is no specific treatment available for EHEC infection and the use of conventional antibiotics is contraindicated. Therefore, identification of potential therapeutic targets and development of effective measures to control and treat EHEC infection are needed. Lipopolysaccharides (LPS) are surface glycolipids found on the outer membrane of gram-negative bacteria, including EHEC, and LPS biosynthesis has long been considered as potential anti-bacterial target. Here, we demonstrated that the EHEC rfaD gene that functions in the biosynthesis of the LPS inner core is required for the intestinal colonization and pathogenesis of EHEC in vivo. Disruption of the EHEC rfaD confers attenuated toxicity in Caenorhabditis elegans and less bacterial colonization in the intestine of C. elegans and mouse. Moreover, rfaD is also involved in the control of susceptibility of EHEC to antimicrobial peptides and host intestinal immunity. It is worth noting that rfaD mutation did not interfere with the growth kinetics when compared to the wild-type EHEC cells. Taken together, we demonstrated that mutations of the EHEC rfaD confer hypersusceptibility to host intestinal innate immunity in vivo, and suggested that targeting the RfaD or the core LPS synthesis pathway may provide alternative therapeutic regimens for EHEC infection. PMID:27570746

  1. Mutation of the Enterohemorrhagic Escherichia coli Core LPS Biosynthesis Enzyme RfaD Confers Hypersusceptibility to Host Intestinal Innate Immunity In vivo

    PubMed Central

    Kuo, Cheng-Ju; Chen, Jenn-Wei; Chiu, Hao-Chieh; Teng, Ching-Hao; Hsu, Tai-I; Lu, Pei-Jung; Syu, Wan-Jr; Wang, Sin-Tian; Chou, Ting-Chen; Chen, Chang-Shi

    2016-01-01

    Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important foodborne pathogen causing severe diseases in humans worldwide. Currently, there is no specific treatment available for EHEC infection and the use of conventional antibiotics is contraindicated. Therefore, identification of potential therapeutic targets and development of effective measures to control and treat EHEC infection are needed. Lipopolysaccharides (LPS) are surface glycolipids found on the outer membrane of gram-negative bacteria, including EHEC, and LPS biosynthesis has long been considered as potential anti-bacterial target. Here, we demonstrated that the EHEC rfaD gene that functions in the biosynthesis of the LPS inner core is required for the intestinal colonization and pathogenesis of EHEC in vivo. Disruption of the EHEC rfaD confers attenuated toxicity in Caenorhabditis elegans and less bacterial colonization in the intestine of C. elegans and mouse. Moreover, rfaD is also involved in the control of susceptibility of EHEC to antimicrobial peptides and host intestinal immunity. It is worth noting that rfaD mutation did not interfere with the growth kinetics when compared to the wild-type EHEC cells. Taken together, we demonstrated that mutations of the EHEC rfaD confer hypersusceptibility to host intestinal innate immunity in vivo, and suggested that targeting the RfaD or the core LPS synthesis pathway may provide alternative therapeutic regimens for EHEC infection. PMID:27570746

  2. Parliamentary Libraries Section. General Research Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on parliamentary and legislative libraries presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Parliamentary Libraries and Networks," a brief overview by Dermot Englefield (United Kingdom) of the position of legislative libraries in national networks, the extent of legislative library…

  3. Public Libraries Section. Libraries Serving General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on public libraries, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Role of Public Libraries in Developing Countries with Particular Reference to the Gambia" by Sally P. C. N'Jie (The Gambia); (2) "Public Libraries in the Federal Republic of Germany (FRG)" by Friedrich…

  4. National Libraries Section. General Research Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on national library services and activities, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The National Library of China in its Gradual Application of Modern Technology," a discussion by Zhu Nan and Zhu Yan (China) of microform usage and library automation; (2) "The…

  5. Academic Libraries: Issues and Trends.

    ERIC Educational Resources Information Center

    O'Brien, Leacy

    1996-01-01

    Discusses issues and trends affecting Canadian academic libraries that were major themes at the 1996 Canadian Library Association annual conference in Halifax (Nova Scotia). Topics include government funding decreases; restructuring in education and library services; consortia; library cooperation and collaboration; computer networks; interlibrary…

  6. A single immunization with a recombinant canine adenovirus expressing the rabies virus G protein confers protective immunity against rabies in mice

    SciTech Connect

    Li Jianwei; Faber, Milosz; Papaneri, Amy; Faber, Marie-Luise; McGettigan, James P.; Schnell, Matthias J.; Dietzschold, Bernhard . E-mail: bernhard.dietzschold@jefferson.edu

    2006-12-20

    Rabies vaccines based on live attenuated rabies viruses or recombinant pox viruses expressing the rabies virus (RV) glycoprotein (G) hold the greatest promise of safety and efficacy, particularly for oral immunization of wildlife. However, while these vaccines induce protective immunity in foxes, they are less effective in other animals, and safety concerns have been raised for some of these vaccines. Because canine adenovirus 2 (CAV2) is licensed for use as a live vaccine for dogs and has an excellent efficacy and safety record, we used this virus as an expression vector for the RVG. The recombinant CAV2-RV G produces virus titers similar to those produced by wild-type CAV2, indicating that the RVG gene does not affect virus replication. Comparison of RVG expressed by CAV2-RV G with that of vaccinia-RV G recombinant virus (V-RG) revealed similar amounts of RV G on the cell surface. A single intramuscular or intranasal immunization of mice with CAV2-RVG induced protective immunity in a dose-dependent manner, with no clinical signs or discomfort from the virus infection regardless of the route of administration or the amount of virus.

  7. Art Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on art libraries and information services for the arts, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "'I See All': Information Technology and the Universal Availability of Images" by Philip Pacey (United Kingdom); (2) "Online Databases in the Fine Arts" by Michael Rinehart…

  8. Mosaic vaccines elicit CD8+ T cell responses in monkeys that confer immune coverage of diverse HIV strains

    SciTech Connect

    Fischer, Will; Korber, Bette

    2009-01-01

    Creation of a successful HIV vaccine will require the development of a strategy to generate cellular immunity with sufficient cross-clade breadth to deal with the extreme genetic diversity of the virus. Polyvalent mosaic immunogens derived from in silica recombination of natural strains of HIV are designed to induce cellular immune responses that maximally cover the sequence diversity of circulating virus isolates. Immunization of rhesus monkeys with plasmid DNA and recombinant vaccinia virus vaccine constructs expressing either consensus immunogens or polyvalent mosaic immunogens elicited a CD4+ T lymphocyte-biased response with comparably broad epitope-specific total T lymphocyte specificities. However, immunization with the mosaic immunogens induced HIV-specific CD8+ T lymphocyte responses with markedly greater depth and breadth. Therefore, the use of polyvalent mosaic immunogens is a promising strategy for a global vaccine for HIV.

  9. Analysis of the immune response against mixotope peptide libraries from a main antigenic site of foot-and-mouth disease virus.

    PubMed

    Oliveira, Eliandre de; Jiménez-Clavero, Miguel Angel; Núñez, José Ignacio; Sobrino, Francisco; Andreu, David

    2005-04-01

    The design of vaccines for RNA viral diseases is complicated by the high genetic variability of the viruses, which favors the selection of escape mutants. A case in point is foot-and-mouth disease virus (FMDV), for which only limited protection has been observed in vaccination with single peptides. We have explored the potential of immunogens of higher sequence diversity, covering a broad range of field or culture-induced mutations at the immunodominant site A of FMDV, serotype C. Four mixotope-type peptide libraries, containing ca. 3 x 10(3) or ca. 3 x 10(5) peptides each, in either linear or cyclic form, and combining most significant mutations found or induced at site A have been synthesized and used to immunize guinea-pigs. Substantial levels of serum conversion have been observed for all four mixotope libraries, as well as for single peptides, linear or cyclic, corresponding to the consensus site A sequence. The specificity and neutralizing ability of the anti-mixotope and -peptide antibodies have been evaluated by direct ELISA and by plaque reduction and micro-neutralization assays, respectively. Challenge experiments with an infectious, guinea-pig-adapted FMDV strain, have shown higher protection rates in animals immunized with the cyclic versions, either in single sequence or in combinatorial mixotope form. PMID:15780448

  10. Analysis of a genomic DNA expression library of Mycobacterium tuberculosis using tuberculosis patient sera: evidence for modulation of host immune response.

    PubMed Central

    Amara, R R; Satchidanandam, V

    1996-01-01

    DNA obtained from a human sputum isolate of Mycobacterium tuberculosis, NTI-64719, which showed extensive dissemination in the guinea pig model resulting in a high score for virulence was used to construct an expression library in the lambda ZAP vector. The size of DNA inserts in the library ranged from 1 to 3 kb, and recombinants represented 60% of the total plaques obtained. When probed with pooled serum from chronically infected tuberculosis patients, the library yielded 176 recombinants with a range of signal intensities. Among these, 93 recombinants were classified into 12 groups on the basis of DNA hybridization experiments. The polypeptides synthesized by the recombinants were predominantly LacZ fusion proteins. Serum obtained from patients who were clinically diagnosed to be in the early phase of M. tuberculosis infection was used to probe the 176 recombinants obtained. Interestingly, some recombinants that gave very strong signals in the original screen did not react with early-phase serum; conversely, other whose signals were extremely weak in the original screen gave very intense signals with serum from recently infected patients. This indicates the differential nature of either the expression of these antigens or the immune response elicited by them as a function of disease progression. PMID:8751927

  11. A single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus Gn glycoprotein confers protective immunity against seoul virus in mice.

    PubMed

    Yuan, Zi-Guo; Li, Xiu-Ming; Mahmmod, Yasser Saad; Wang, Xiao-Hu; Xu, Hui-Juan; Zhang, Xiu-Xiang

    2009-08-20

    Seoul virus (SEOV), a member of hantavirus genus, is one of the causative agents of hemorrhagic fever with renal syndrome (HFRS) and afflicts tens of thousands of people annually. In this paper, we evaluate the immune response induced by a replication-competent recombinant canine adenovirus type 2 expressing the Gn protein of SEOV (rCAV-2-Gn) in BALB/c mice. Sera from immunized mice contained neutralizing antibodies that could specifically recognize SEOV and neutralize its infectivity in vitro. Moreover, the recombinant virus induced complete protection against a lethal challenge with the highly virulent SEOV strain CC-2. Protective level neutralizing antibodies were maintained for at least 20 weeks. The efficacy of the recombinant was similar to that induced by a currently available inactivated HFRS vaccine. This recombinant virus is therefore a potential alternative to the inactivated vaccine.

  12. Using the local immune response from the natural buffalo host to generate an antibody fragment library that binds the early larval stages of Schistosoma japonicum.

    PubMed

    Hosking, Christopher G; Driguez, Patrick; McWilliam, Hamish E G; Ilag, Leodevico L; Gladman, Simon; Li, Yuesheng; Piedrafita, David; McManus, Donald P; Meeusen, Els N T; de Veer, Michael J

    2015-09-01

    Antibodies isolated from the local draining inguinal lymph node of field exposed-water buffaloes following challenge with Schistosoma japonicum cercariae showed high reactivity towards S. japonicum antigen preparations and bound specifically to formaldehyde-fixed S. japonicum schistosomules. Using this specific local immune response we produced a series of single-chain antibody Fv domain libraries from the same lymph nodes. Removal of phage that cross reacted with epitopes on adult parasites yielded a single-chain antibody Fv domain-phage library that specifically bound to whole formaldehyde-fixed and live S. japonicum schistosomules. DNA sequencing indicated clear enrichment of the single-chain antibody Fv domain library for buffalo B-cell complementarity determining regions post-selection for schistosomule binding. This study also revealed that long heavy chain complementarity determining regions appear to be an important factor when selecting for antibody binding fragments against schistosomule proteins. The selected single-chain antibody Fv domain-phage were used to probe a schistosome-specific protein microarray, which resulted in the recognition of many proteins expressed across all schistosome life-cycle stages. Following absorption to adult worms, the single-chain antibody Fv domain-phage library showed significantly reduced binding to most proteins, whilst two proteins (NCBI GenBank accession numbers AY915878 and AY815196) showed increased binding. We have thus developed a unique set of host derived single-chain antibody Fv domains comprising buffalo B-cell variable regions that specifically bind to early S. japonicum life-stages.

  13. SLA at 100: Conference Preview

    ERIC Educational Resources Information Center

    Blumenstein, Lynn

    2009-01-01

    When School Library Association (SLA) convenes its annual conference in Washington, DC, June 14-17, 2009, the association will be celebrating its 100th birthday. This occasion allows for grand gestures--the SLA Salutes! Awards and Leadership Reception will be held in the Library of Congress's Great Hall. The conference also draws upon Washington…

  14. Selection of an antibody library identifies a pathway to induce immunity by targeting CD36 on steady-state CD8 alpha+ dendritic cells.

    PubMed

    Tagliani, Elisa; Guermonprez, Pierre; Sepúlveda, Jorge; López-Bravo, María; Ardavín, Carlos; Amigorena, Sebastian; Benvenuti, Federica; Burrone, Oscar R

    2008-03-01

    Improvement of the strategy to target tumor Ags to dendritic cells (DCs) for immunotherapy requires the identification of the most appropriate ligand/receptor pairing. We screened a library of Ab fragments on mouse DCs to isolate new potential Abs capable of inducing protective immune responses. The screening identified a high-affinity Ab against CD36, a multi-ligand scavenger receptor primarily expressed by the CD8alpha+ subset of conventional DCs. The Ab variable regions were genetically linked to the model Ag OVA and tested in Ag presentation assays in vitro and in vivo. Anti-CD36-OVA was capable of delivering exogenous Ags to the MHC class I and MHC class II processing pathways. In vivo, immunization with anti-CD36-OVA induced robust activation of naive CD4+ and CD8+ Ag-specific T lymphocytes and the differentiation of primed CD8+ T cells into long-term effector CTLs. Vaccination with anti-CD36-OVA elicited humoral and cell-mediated protection from the growth of an Ag-specific tumor. Notably, the relative efficacy of targeting CD11c/CD8alpha+ via CD36 or DEC205 was qualitatively different. Anti-DEC205-OVA was more efficient than anti-CD36-OVA in inducing early events of naive CD8+ T cell activation. In contrast, long-term persistence of effector CTLs was stronger following immunization with anti-CD36-OVA and did not require the addition of exogenous maturation stimuli. The results identify CD36 as a novel potential target for immunotherapy and indicate that the outcome of the immune responses vary by targeting different receptors on CD8alpha+ DCs.

  15. On the Conference Circuit.

    ERIC Educational Resources Information Center

    Tyckoson, David A.

    1987-01-01

    Summarizes three conference presentations on the effects of the economic climate on academic libraries in Iowa. These presentations focused on the impact of austerity budgets on collection development, library services and personnel, and possible management approaches to retrenchment in these areas. (CLB)

  16. Voyaging from the Past, to the Present, and into the Future: Knowing Your Heritage. Selected Papers from PIALA 2003, Pacific Islands Association of Libraries, Archives and Museums Annual Conference (13th, Pohnpei, Federated States of Micronesia, November 4-6, 2003)

    ERIC Educational Resources Information Center

    Drake, Paul B., Ed.

    2003-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 13th annual conference was held in Pohnpei, Federated States of Micronesia, November 4-6, 2003. The volume begins with a listing of the members of the PIALA 2003 Planning &…

  17. Vaccination with Lipid Core Peptides Fails to Induce Epitope-Specific T Cell Responses but Confers Non-Specific Protective Immunity in a Malaria Model

    PubMed Central

    Apte, Simon H.; Groves, Penny L.; Skwarczynski, Mariusz; Fujita, Yoshio; Chang, Chenghung; Toth, Istvan; Doolan, Denise L.

    2012-01-01

    Vaccines against many pathogens for which conventional approaches have failed remain an unmet public health priority. Synthetic peptide-based vaccines offer an attractive alternative to whole protein and whole organism vaccines, particularly for complex pathogens that cause chronic infection. Previously, we have reported a promising lipid core peptide (LCP) vaccine delivery system that incorporates the antigen, carrier, and adjuvant in a single molecular entity. LCP vaccines have been used to deliver several peptide subunit-based vaccine candidates and induced high titre functional antibodies and protected against Group A streptococcus in mice. Herein, we have evaluated whether LCP constructs incorporating defined CD4+ and/or CD8+ T cell epitopes could induce epitope-specific T cell responses and protect against pathogen challenge in a rodent malaria model. We show that LCP vaccines failed to induce an expansion of antigen-specific CD8+ T cells following primary immunization or by boosting. We further demonstrated that the LCP vaccines induced a non-specific type 2 polarized cytokine response, rather than an epitope-specific canonical CD8+ T cell type 1 response. Cytotoxic responses of unknown specificity were also induced. These non-specific responses were able to protect against parasite challenge. These data demonstrate that vaccination with lipid core peptides fails to induce canonical epitope-specific T cell responses, at least in our rodent model, but can nonetheless confer non-specific protective immunity against Plasmodium parasite challenge. PMID:22936972

  18. Microneedle delivery of an M2e-TLR5 ligand fusion protein to skin confers broadly cross-protective influenza immunity.

    PubMed

    Wang, Bao-Zhong; Gill, Harvinder S; He, Cheng; Ou, Changbo; Wang, Li; Wang, Ying-Chun; Feng, Hao; Zhang, Han; Prausnitz, Mark R; Compans, Richard W

    2014-03-28

    Influenza vaccines with broad cross-protection are urgently needed to prevent an emerging influenza pandemic. A fusion protein of the Toll-like receptor (TLR) 5-agonist domains from flagellin and multiple repeats of the conserved extracellular domain of the influenza matrix protein 2 (M2e) was constructed, purified and evaluated as such a vaccine. A painless vaccination method suitable for possible self-administration using coated microneedle arrays was investigated for skin-targeted delivery of the fusion protein in a mouse model. The results demonstrate that microneedle immunization induced strong humoral as well as mucosal antibody responses and conferred complete protection against homo- and heterosubtypic lethal virus challenges. Protective efficacy with microneedles was found to be significantly better than that seen with conventional intramuscular injection, and comparable to that observed with intranasal immunization. Because of its advantages for administration, safety and storage, microneedle delivery of M2e-flagellin fusion protein is a promising approach for an easy-to-administer universal influenza vaccine. PMID:24417966

  19. Nebraska Information Partnerships, A Report to Nebraskans. Nebraska Conference on Library and Information Services (Omaha, Nebraska, February 24-26, 1991).

    ERIC Educational Resources Information Center

    Nebraska Library Commission, Lincoln.

    This report begins by describing the activities of the members of the Nebraska Information Partnerships Planning Committee as they planned for the state conference, including a Delphi survey that was conducted to identify key issues and direction for discussion at the conference, and the selection of 100 delegates. Conference activities and…

  20. International Federation of Library Associations Annual Conference Papers. Education and Research Division: Editors of Library Journals and Serial Publications Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Bankole, E. Bejide; And Others

    Four papers and two reports from three sections concerned with library science periodicals and serials publications in libraries include discussions of the role of library and information science journals and their coverage by indexing services as well as a description of an online system for serials, a report from the working group on newspapers,…

  1. Innate immune memory and homeostasis may be conferred through crosstalk between the TLR3 and TLR7 pathways

    PubMed Central

    Liu, Bing; Liu, Qian; Yang, Lei; Palaniappan, Sucheendra K.; Bahar, Ivet; Thiagarajan, P. S.; Ding, Jeak Ling

    2016-01-01

    Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns (PAMPs) and stimulate the innate immune response through the production of cytokines. The innate immune response depends on the timing of encountering PAMPs, suggesting a short-term “memory.” In particular, activation of TLR3 appears to prime macrophages for the subsequent activation of TLR7, which leads to synergistically increased production of cytokines. By developing a calibrated mathematical model for the kinetics of TLR3 and TLR7 pathway crosstalk and providing experimental validation, we demonstrated the involvement of the Janus-activated kinase (JAK)–signal transducer and activator of transcription (STAT) pathway in controlling the synergistic production of cytokines. Signaling through this pathway played a dual role: It mediated the synergistic production of cytokines, thus boosting the immune response, and it also maintained homeostasis to avoid an excessive inflammatory response. Thus, we propose that the JAK-STAT pathway provides a cytokine rheostat mechanism, which enables macrophages to fine-tune their responses to multiple, temporally separated infection events involving the TLR3 and TLR7 pathways. PMID:27405980

  2. ALA Conference 2009: Chicago Hope

    ERIC Educational Resources Information Center

    Berry, John N., III

    2009-01-01

    There is joy among those who have the funds to go to Chicago for the American Library Association (ALA) annual conference, July 9-15. Every librarian knows there is nothing better than a Chicago gathering, with the city's wonderful haunts, museums, restaurants, and fine memories of past conferences. The conference program covers nearly every…

  3. IOLS '98. Proceedings of the National Conference on Integrated Online Library Systems (13th, New York, New York, May 13-14, 1998).

    ERIC Educational Resources Information Center

    Cibbarelli, Pamela R., Comp.; Nixon, Carol, Comp.

    Integrated Online Library Systems (IOLS) focuses exclusively on the issues of planning and managing automated systems in libraries and information centers. The theme of the IOLS '98 annual meeting is "Embrace and Extend," and 18 papers were selected for presentation that provide insight into the current technologies being "embraced" in libraries.…

  4. International Federation of Library Associations Annual Conference. Papers of the Management and Technology Division: Information Technology Section (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Bradler, Reinhard; And Others

    These seven papers on library management and networks focus on: (1) computerized access to archival and library materials, describing the methodological problems associated with a pilot project in the German Democratic Republic, as well as the efficiency of data bank systems; (2) present and future development of libraries and information centers…

  5. Mechanism of eliciting host immunity against cancer cells treated with silica-phthalocyanine-based near infrared photoimmunotherapy (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Kobayashi, Hisataka

    2016-03-01

    Near infrared (NIR) photoimmunotherapy (PIT) is a new type of molecularly-targeted cancer photo-therapy based on conjugating a near infrared silica-phthalocyanine dye, IR700, to a monoclonal antibody (MAb) targeting cancer-specific cell-surface molecules. When exposed to NIR light, the conjugate induces a highly-selective necrotic/ immunogenic cell death (ICD) only in receptor-positive, MAb-IR700-bound cancer cells. This cell death occurs as early as 1 minute after exposure to NIR light. Meanwhile, immediately adjacent receptor-negative cells including immune cells are unharmed. Therefore, we hypothesized that NIR-PIT could efficiently elicit host immunity against treated cancer cells. Three-dimensional dynamic quantitative phase contrast microscopy and selective plane illumination microscopy of tumor cells undergoing PIT showed rapid swelling in treated cells immediately after light exposure suggesting rapid water influx into cells, followed by irreversible morphologic changes such as bleb formation, and rupture of vesicles. Furthermore, biological markers of ICD including relocation of HSP70/90 and calreticulin, and release of ATP and High Mobility Group Box 1 (HMGB1), were clearly detected immediately after NIR-PIT. When NIR-PIT was performed in a mixture of cancer cells and immature dendritic cells, maturation of immature dendritic cells was strongly induced rapidly after NIR-PIT. In summary, NIR-PIT can induce necrotic/ immunogenic cell death that promotes rapid maturation of immature dendritic cells adjacent to dying cancer cells. Therefore, NIR-PIT could efficiently initiate host immune response against NIR-PIT treated cancer cells growing in patients.

  6. Calnexin induces expansion of antigen-specific CD4+ T cells that confer immunity to fungal ascomycetes via conserved epitopes

    PubMed Central

    Wüthrich, Marcel; Brandhorst, Tristan T.; Sullivan, Thomas D.; Filutowicz, Hanna; Sterkel, Alana; Stewart, Douglas; Li, Mengyi; Lerksuthirat, Tassanee; LeBert, Vanessa; Shen, Zu Ting; Ostroff, Gary; Deepe, George S.; Hung, Chiung Yu; Cole, Garry; Walter, Jennifer A.; Jenkins, Marc K.; Klein, Bruce

    2015-01-01

    Fungal infections remain a threat due to the lack of broad spectrum fungal vaccines and protective antigens. Recent studies showed that attenuated Blastomyces dermatitidis confers protection via T cell recognition of an unknown, but conserved antigen. Using transgenic CD4+ T cells recognizing this antigen, we identify an amino acid determinant within the chaperone calnexin that is conserved across diverse fungal ascomycetes. Calnexin, typically an ER protein, also localizes to the surface of yeast, hyphae and spores. T cell epitope mapping unveiled a 13-residue sequence conserved across Ascomycota. Infection with divergent ascomycetes including dimorphic fungi, opportunistic molds, and the agent causing white nose syndrome in bats induces expansion of calnexin-specific CD4+ T cells. Vaccine delivery of calnexin in glucan particles induces fungal antigen-specific CD4+ T cell expansion and resistance to lethal challenge with multiple fungal pathogens. Thus, the immunogeneticity and conservation of calnexin make this fungal protein a promising vaccine target. PMID:25800545

  7. Calnexin induces expansion of antigen-specific CD4(+) T cells that confer immunity to fungal ascomycetes via conserved epitopes.

    PubMed

    Wüthrich, Marcel; Brandhorst, Tristan T; Sullivan, Thomas D; Filutowicz, Hanna; Sterkel, Alana; Stewart, Douglas; Li, Mengyi; Lerksuthirat, Tassanee; LeBert, Vanessa; Shen, Zu Ting; Ostroff, Gary; Deepe, George S; Hung, Chiung Yu; Cole, Garry; Walter, Jennifer A; Jenkins, Marc K; Klein, Bruce

    2015-04-01

    Fungal infections remain a threat due to the lack of broad-spectrum fungal vaccines and protective antigens. Recent studies showed that attenuated Blastomyces dermatitidis confers protection via T cell recognition of an unknown but conserved antigen. Using transgenic CD4(+) T cells recognizing this antigen, we identify an amino acid determinant within the chaperone calnexin that is conserved across diverse fungal ascomycetes. Calnexin, typically an ER protein, also localizes to the surface of yeast, hyphae, and spores. T cell epitope mapping unveiled a 13-residue sequence conserved across Ascomycota. Infection with divergent ascomycetes, including dimorphic fungi, opportunistic molds, and the agent causing white nose syndrome in bats, induces expansion of calnexin-specific CD4(+) T cells. Vaccine delivery of calnexin in glucan particles induces fungal antigen-specific CD4(+) T cell expansion and resistance to lethal challenge with multiple fungal pathogens. Thus, the immunogenicity and conservation of calnexin make this fungal protein a promising vaccine target. PMID:25800545

  8. Children's Libraries Section. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on school and children's libraries which were presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Learning to Learn in the Street," a description by B. Lang (France) of "street libraries" and other institutions designed to introduce economically disadvantaged children to books and other…

  9. Parliamentary Libraries Section. General Research Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on parliamentary and legislative libraries, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "Need for and Use of Parliamentary Papers," a report by Eeva-Maija Tammekann on the results of a 1983 survey of 37 parliamentary libraries (with a 51% response rate) covering types of…

  10. Science and Technology Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on science and technology library and information services presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "The Central Subject Libraries of the Federal Republic of Germany--For Example: The Technische Informationsbibliothek Hannover" by Gerhard Schlitt and Jobst Tehnzen; (2) "The…

  11. Geography and Map Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on geography and map libraries and a paper on European access to biomedical databases, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "History and Current Status of the Globe Museum of the Austrian National Library" by Franz Wawrik (Austria); (2) "Earth Science Products for…

  12. Science and Technology Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on scientific/technical information and libraries, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "Patents as Information--An Unused Resource" by Richard D. Walker (United States); (2) "Survey of the Information Services of the Library of the German Patent Office" by Eckhard…

  13. Subcutaneous immunization with a novel immunogenic candidate (urease) confers protection against Brucella abortus and Brucella melitensis infections.

    PubMed

    Abkar, Morteza; Amani, Jafar; Sahebghadam Lotfi, Abbas; Nikbakht Brujeni, Gholamreza; Alamian, Saeed; Kamali, Mehdi

    2015-08-01

    Brucellosis is a world prevalent endemic illness that is transmitted from domestic animals to humans. Brucella spp. exploits urease for survival in the harsh conditions of stomach during the gastrointestinal infection. In this study, we examined the immune response and the protection elicited by using recombinant Brucella urease (rUrease) vaccination in BALB/c mice. The urease gene was cloned in pET28a and the resulting recombinant protein was employed as subunit vaccine. Recombinant protein was administered subcutaneously and intraperitoneally. Dosage reduction was observed with subcutaneous (SC) vaccination when compared with intraperitoneal (IP) vaccination. rUrease induced mixed Th1-Th2 immune responses with high titers of specific IgG1 and IgG2a. In lymphocyte proliferation assay, splenocytes from IP and SC-vaccinated mice displayed a strong recall proliferative response with high amounts of IL-4, IL-12 and IFN-γ production. Vaccinated mice were challenged with virulent Brucella melitensis, B. abortus and B. suis. The SC vaccination route exhibited a higher degree of protection than IP vaccination (p value ≤ 0.05). Altogether, our results indicated that rUrease could be a useful antigen candidate for the development of subunit vaccines against brucellosis.

  14. Cgnz1 allele confers kidney resistance to damage preventing progression of immune complex-mediated acute lupus glomerulonephritis.

    PubMed

    Ge, Yan; Jiang, Chao; Sung, Sun-Sang J; Bagavant, Harini; Dai, Chao; Wang, Hongyang; Kannapell, Carol C; Cathro, Helen P; Gaskin, Felicia; Fu, Shu Man

    2013-10-21

    Cgnz1 and Agnz1 on the distal region of mouse chromosome 1 are associated with chronic glomerulonephritis (cGN) and acute GN (aGN). NZM2328.Lc1R27 (R27) was generated by introgressing a C57L/J region where Cgnz1 is located to NZM2328. R27 female mice developed aGN mediated by immune complex (IC) deposition and complement activation without progression to cGN with severe proteinuria. End stage renal disease (ESRD) was not seen in R27 mice as old as 15 mo. Thus, aGN and cGN are under separate genetic control, and IC-mediated proliferative GN need not progress to cGN and ESRD. NZM2328 and R27 female mice have comparable immune and inflammatory parameters. In contrast to NZM2328, R27 mice were resistant to sheep anti-mouse GBM serum-induced nephritis, supporting the hypothesis that aGN is mediated by autoimmunity and resistance to the development of cGN is mediated by end organ resistance to damage. Thus, autoimmunity should be considered distinct from end organ damage. The Cgnz1 region has been mapped to a 1.34 MB region with 45 genes. Nine candidate genes were identified. Clinical relevance of these observations is supported by case studies. Clinical implications and the significance to human lupus and other diseases are presented.

  15. Macintoshed Libraries 2.0.

    ERIC Educational Resources Information Center

    Vaccaro, Bill, Ed.; Valauskas, Edward J., Ed.

    This annual collection contains 18 papers about the use of Macintosh computers in libraries. Papers include: "The Macintosh as a Wayfinding Tool for Professional Conferences: The LITA '88 HyperCard Stack" (Ann F. Bevilacqua); "Enhancing Library Services with the Macintosh" (Naomi C. Broering); "Scanning Technologies in Libraries" (Steve Cisler);…

  16. Comprehensive Library Planning Program Prospectus.

    ERIC Educational Resources Information Center

    Southeastern Wisconsin Regional Planning Commission, Waukesha.

    At the request of the Southeastern Wisconsin Regional Library Conference, the Southeastern Wisconsin Regional Planning Commission created a Technical Advisory Committee on Library Planning to assist the Commission staff in preparing a prospectus for a long-range, area-wide public library facilities and services plan. The Technical Advisor…

  17. Library Theory and Research Section. Round Table on Library History. Education and Research Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on theory and research in library history presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "The Future of the Library's Past: Library Administrative Records Preservation, Policy and Practice," a discussion by Paul Sturges (United Kingdom) of the need for library records preservation,…

  18. Oral Vaccination with Salmonella enterica as a Cruzipain-DNA Delivery System Confers Protective Immunity against Trypanosoma cruzi▿

    PubMed Central

    Cazorla, Silvia I.; Becker, Pablo D.; Frank, Fernanda M.; Ebensen, Thomas; Sartori, María J.; Corral, Ricardo S.; Malchiodi, Emilio L.; Guzmán, Carlos A.

    2008-01-01

    To stimulate both local and systemic immune responses against Trypanosoma cruzi, Salmonella enterica serovar Typhimurium aroA was exploited as a DNA delivery system for cruzipain (SCz). In a murine model we compared SCz alone (GI) or coadministered with Salmonella carrying a plasmid encoding granulocyte-macrophage colony-stimulating factor (GII), as well as protocols in which SCz priming was followed by boosting with recombinant cruzipain (rCz) admixed with either CpG-ODN (GIII) or MALP-2, a synthetic derivative of a macrophage-activating lipopeptide of 2 kDa from Mycoplasma fermentans (GIV). The results showed that protocols that included four oral doses of SCz (GI) elicited mainly a mucosal response characterized by immunoglobulin A (IgA) secretion and proliferation of gut-associated lymphoid tissue cells, with weak systemic responses. In contrast, the protocol that included a boost with rCz plus CpG (GIII) triggered stronger systemic responses in terms of Cz-specific serum IgG titers, splenocyte proliferation, gamma interferon (IFN-γ) secretion, and delayed-type hypersensitivity response. Trypomastigote challenge of vaccinated mice resulted in significantly lower levels of parasitemia compared to controls. Protection was abolished by depletion of either CD4+ or CD8+ T cells. Parasite control was also evident from the reduction of tissue damage, as revealed by histopathologic studies and serum levels of enzymes that are markers of muscle injury in chronic Chagas' disease (i.e., creatine kinase, aspartate aminotransferase, and lactate dehydrogenase). Enhanced release of IFN-γ and interleukin-2 was observed in GI and GII upon restimulation of splenocytes in the nonparasitic phase of infection. Our results indicate that Salmonella-mediated delivery of Cz-DNA by itself promotes the elicitation of an immune response that controls T. cruzi infection, thereby reducing parasite loads and subsequent damage to muscle tissues. PMID:17967857

  19. Immunization of Mice with Recombinant Protein CobB or AsnC Confers Protection against Brucella abortus Infection

    PubMed Central

    Du, Xinying; Yu, Shuang; Bai, Yaoxia; Chen, Yanfen; Wang, Tongkun; Wang, Zhoujia; Yu, Yaqing; Peng, Guangneng; Huang, Kehe; Huang, Liuyu; Wang, Yufei; Chen, Zeliang

    2012-01-01

    Due to drawbacks of live attenuated vaccines, much more attention has been focused on screening of Brucella protective antigens as subunit vaccine candidates. Brucella is a facultative intracellular bacterium and cell mediated immunity plays essential roles for protection against Brucella infection. Identification of Brucella antigens that present T-cell epitopes to the host could enable development of such vaccines. In this study, 45 proven or putative pathogenesis-associated factors of Brucella were selected according to currently available data. After expressed and purified, 35 proteins were qualified for analysis of their abilities to stimulate T-cell responses in vitro. Then, an in vitro gamma interferon (IFN-γ) assay was used to identify potential T-cell antigens from B. abortus. In total, 7 individual proteins that stimulated strong IFN-γ responses in splenocytes from mice immunized with B. abortus live vaccine S19 were identified. The protective efficiencies of these 7 recombinant proteins were further evaluated. Mice given BAB1_1316 (CobB) or BAB1_1688 (AsnC) plus adjuvant could provide protection against virulent B. abortus infection, similarly with the known protective antigen Cu-Zn SOD and the license vaccine S19. In addition, CobB and AsnC could induce strong antibodies responses in BALB/c mice. Altogether, the present study showed that CobB or AsnC protein could be useful antigen candidates for the development of subunit vaccines against brucellosis with adequate immunogenicity and protection efficacy. PMID:22383953

  20. Immune responses associated with homologous protection conferred by commercial vaccines for control of avian pathogenic Escherichia coli in turkeys.

    PubMed

    Sadeyen, Jean-Rémy; Wu, Zhiguang; Davies, Holly; van Diemen, Pauline M; Milicic, Anita; La Ragione, Roberto M; Kaiser, Pete; Stevens, Mark P; Dziva, Francis

    2015-01-01

    Avian pathogenic Escherichia coli (APEC) infections are a serious impediment to sustainable poultry production worldwide. Licensed vaccines are available, but the immunological basis of protection is ill-defined and a need exists to extend cross-serotype efficacy. Here, we analysed innate and adaptive responses induced by commercial vaccines in turkeys. Both a live-attenuated APEC O78 ΔaroA vaccine (Poulvac® E. coli) and a formalin-inactivated APEC O78 bacterin conferred significant protection against homologous intra-airsac challenge in a model of acute colibacillosis. Analysis of expression levels of signature cytokine mRNAs indicated that both vaccines induced a predominantly Th2 response in the spleen. Both vaccines resulted in increased levels of serum O78-specific IgY detected by ELISA and significant splenocyte recall responses to soluble APEC antigens at post-vaccination and post-challenge periods. Supplementing a non-adjuvanted inactivated vaccine with Th2-biasing (Titermax® Gold or aluminium hydroxide) or Th1-biasing (CASAC or CpG motifs) adjuvants, suggested that Th2-biasing adjuvants may give more protection. However, all adjuvants tested augmented humoral responses and protection relative to controls. Our data highlight the importance of both cell-mediated and antibody responses in APEC vaccine-mediated protection toward the control of a key avian endemic disease.

  1. University Libraries and Other General Research Libraries Section. General Research Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on university and other research libraries, presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Impact of Technology on Users of Academic and Research Libraries," in which C. Lee Jones (United States) focuses on the impact of technical advances in computing and telecommunication…

  2. Kallistatin Modulates Immune Cells and Confers Anti-Inflammatory Response To Protect Mice from Group A Streptococcal Infection

    PubMed Central

    Lu, Shiou-Ling; Tsai, Chiau-Yuang; Luo, Yueh-Hsia; Kuo, Chih-Feng; Lin, Wei-Chieh; Chang, Yu-Tzu; Wu, Jiunn-Jong; Chuang, Woei-Jer; Liu, Ching-Chuan; Chao, Lee; Chao, Julie

    2013-01-01

    Group A streptococcus (GAS) infection may cause severe life-threatening diseases, including necrotizing fasciitis and streptococcal toxic shock syndrome. Despite the availability of effective antimicrobial agents, there has been a worldwide increase in the incidence of invasive GAS infection. Kallistatin (KS), originally found to be a tissue kallikrein-binding protein, has recently been shown to possess anti-inflammatory properties. However, its efficacy in microbial infection has not been explored. In this study, we transiently expressed the human KS gene by hydrodynamic injection and investigated its anti-inflammatory and protective effects in mice via air pouch inoculation of GAS. The results showed that KS significantly increased the survival rate of GAS-infected mice. KS treatment reduced local skin damage and bacterial counts compared with those in mice infected with GAS and treated with a control plasmid or saline. While there was a decrease in immune cell infiltration of the local infection site, cell viability and antimicrobial factors such as reactive oxygen species actually increased after KS treatment. The efficiency of intracellular bacterial killing in neutrophils was directly enhanced by KS administration. Several inflammatory cytokines, including tumor necrosis factor alpha, interleukin 1β, and interleukin 6, in local infection sites were reduced by KS. In addition, KS treatment reduced vessel leakage, bacteremia, and liver damage after local infection. Therefore, our study demonstrates that KS provides protection in GAS-infected mice by enhancing bacterial clearance, as well as reducing inflammatory responses and organ damage. PMID:23959316

  3. Virtual Libraries: Virtual Communities. Abstracts, Fulltext Documents and PowerPoint Presentations of Papers and Demos Given at the International Association of Technological University Libraries (IATUL) Conference (Brisbane, Queensland, Australia, July 3-7, 2000).

    ERIC Educational Resources Information Center

    International Association of Technological Univ. Libraries, Gothenburg (Sweden).

    This proceedings of the International Association of Technological University Libraries (IATUL) contains the opening address by IATUL president Nancy Fjallbrant and the full text of the following papers: "Building Info-Skills by Degrees: Embedding Information Literacy in University Study" (Wendy Abbott and Deborah Peach); "UQ Library's First CIS:…

  4. The isolation and characterisation of human monoclonal HLA-A2 antibodies from an immune V gene phage display library.

    PubMed

    Watkins, N A; Brown, C; Hurd, C; Navarrete, C; Ouwehand, W H

    2000-03-01

    Molecular cloning techniques and V gene phage display have revolutionised the production of human monoclonal antibodies. Antibodies of a defined specificity can be obtained by selecting phage display libraries on antigen in a process known as panning. We have applied these techniques to the isolation of three HLA-A2-specific single chain variable domain fragments (scFv) from a patient alloimmunised by blood transfusion. Analysis of specificity with cells of HLA genotyped donors revealed the following: i) in addition to the major reactivity with HLA-A2, cross-reactivity with the HLA-A28 epitope; and ii) inhibition of scFv binding to the antigen by the patients' antibodies. The heavy chain variable genes of all three were derived from the germline gene Cos-3, carry the hallmarks of somatic hypermutation, and are most likely derived from clonally related B cells. The light chain variable domains were encoded by DPK1 and DPK8 from the VkappaI family. These data show that phage display can be used to clone HLA-specific alloantibodies that recognise the native antigen from alloimmunised patients. PMID:10777097

  5. CRISPR/cas Loci of Type II Propionibacterium acnes Confer Immunity against Acquisition of Mobile Elements Present in Type I P. acnes

    PubMed Central

    Brüggemann, Holger; Lomholt, Hans B.; Tettelin, Hervé; Kilian, Mogens

    2012-01-01

    Propionibacterium acnes is a skin commensal that occasionally acts as an opportunistic pathogen. The population structure of this species shows three main lineages (I–III). While type I strains are mainly associated with sebaceous follicles of human skin and inflammatory acne, types II and III strains are more often associated with deep tissue infections. We investigated the occurrence and distribution of the clustered regularly interspaced short palindromic repeats (CRISPR) in P. acnes, assessed their immunological memory, and addressed the question if such a system could account for type-specific properties of the species. A collection of 108 clinical isolates covering all known phylotypes of P. acnes was screened for the existence of CRISPR/cas loci. We found that CRISPR loci are restricted to type II P. acnes strains. Sequence analyses of the CRISPR spacers revealed that the system confers immunity to P. acnes-specific phages and to two mobile genetic elements. These elements are found almost exclusively in type I P. acnes strains. Genome sequencing of a type I P. acnes isolate revealed that one element, 54 kb in size, encodes a putative secretion/tight adherence (TAD) system. Thus, CRISPR/cas loci in P. acnes recorded the exposure of type II strains to mobile genetic elements of type I strains. The CRISPR/cas locus is deleted in type I strains, which conceivably accounts for their ability to horizontally acquire fitness or virulence traits and might indicate that type I strains constitute a younger subpopulation of P. acnes. PMID:22479553

  6. Arabidopsis nonhost resistance gene PSS1 confers immunity against an oomycete and a fungal pathogen but not a bacterial pathogen that cause diseases in soybean

    PubMed Central

    2012-01-01

    Background Nonhost resistance (NHR) provides immunity to all members of a plant species against all isolates of a microorganism that is pathogenic to other plant species. Three Arabidopsis thaliana PEN (penetration deficient) genes, PEN1, 2 and 3 have been shown to provide NHR against the barley pathogen Blumeria graminis f. sp. hordei at the prehaustorial level. Arabidopsis pen1-1 mutant lacking the PEN1 gene is penetrated by the hemibiotrophic oomycete pathogen Phytophthora sojae, the causal organism of the root and stem rot disease in soybean. We investigated if there is any novel nonhost resistance mechanism in Arabidopsis against the soybean pathogen, P. sojae. Results The P.sojaesusceptible (pss) 1 mutant was identified by screening a mutant population created in the Arabidopsis pen1-1 mutant that lacks penetration resistance against the non adapted barley biotrophic fungal pathogen, Blumeria graminis f. sp. hordei. Segregation data suggested that PEN1 is not epistatic to PSS1. Responses of pss1 and pen1-1 to P. sojae invasion were distinct and suggest that PSS1 may act at both pre- and post-haustorial levels, while PEN1 acts at the pre-haustorial level against this soybean pathogen. Therefore, PSS1 encodes a new form of nonhost resistance. The pss1 mutant is also infected by the necrotrophic fungal pathogen, Fusarium virguliforme, which causes sudden death syndrome in soybean. Thus, a common NHR mechanism is operative in Arabidopsis against both hemibiotrophic oomycetes and necrotrophic fungal pathogens that are pathogenic to soybean. However, PSS1 does not play any role in immunity against the bacterial pathogen, Pseudomonas syringae pv. glycinea, that causes bacterial blight in soybean. We mapped PSS1 to a region very close to the southern telomere of chromosome 3 that carries no known disease resistance genes. Conclusions The study revealed that Arabidopsis PSS1 is a novel nonhost resistance gene that confers a new form of nonhost resistance against both

  7. Standards for Library Schools, 1976. Update: A Preliminary Report to the Standing Committee on Education and Training (64th IFLA General Conference, Amsterdam, August 1998).

    ERIC Educational Resources Information Center

    Daniel, Evelyn; Lazinger, Susan

    Several decisions have been made with regard to the general structure and direction of the planned update of IFLA (International Federation of Library Associations and Institutions) Standards for Library Schools, 1976. The changes planned fall into two categories: structural and conceptual. Structural changes would reduce the number of categories…

  8. International Conference of Directors of National Libraries on Resource Sharing in Asia and Oceania, Canberra, 1979: Papers from Australasia and Oceania.

    ERIC Educational Resources Information Center

    Ronnie, Mary; And Others

    1980-01-01

    Describes four library resource sharing projects in (1) New Zealand, (2) Papua New Guinea, (3) Australia, and (4) Fiji. Numerous shared services are discussed, including national bibliographies, publications exchanges, staff exchanges, clearing centers for duplicates, library planning, and national collections. (LLS)

  9. Construction and analysis of a Vibrio cholerae delta-aminolevulinic acid auxotroph which confers protective immunity in a rabbit model.

    PubMed Central

    Rijpkema, S G; Bik, E M; Jansen, W H; Gielen, H; Versluis, L F; Stouthamer, A H; Guinée, P A; Mooi, F R

    1992-01-01

    Vibrio cholerae CVD101 is a very effective live vaccine. Although this strain does not produce active cholera toxin because of a mutation in the gene for the cholera toxin A subunit, it still shows residual pathogenicity. To attenuate CVD101 further, we set out to isolate derivatives of CVD101 which were limited in their ability to proliferate in vivo. Two delta-aminolevulinic acid auxotrophs of CVD101, designated V286 and V287, were isolated by transposon mutagenesis and penicillin enrichment. Southern blotting revealed that the mutants differed with respect to the location of the transposon insertion. Under aerobic conditions, in the absence of delta-aminolevulinic acid, both mutants showed diminished growth compared with CVD101. The growth of V286 was most severely affected. Microaerophilic growth of both mutants was less affected. Competition experiments with a rabbit model showed that strain V286 was found in numbers 10(3)- to 10(4)-fold lower than its parental strain. This observation indicates that strain V286 is impaired in its ability to colonize the rabbit intestine. It also supports an important role for aerobic growth in the colonization of the intestine by V. cholerae. Vaccination of rabbits with a single dose of strain V286 resulted in full protection against challenge with a virulent strain. Strain V286 was not shed from rabbits in a cultivatable form. Our results suggest that delta-aminolevulinic acid auxotrophy can attenuate V. cholerae by limiting its ability to colonize without affecting its capacity to induce protective immunity. Furthermore, this type of mutation may prevent the spread of V. cholerae vaccine strains in the environment. Images PMID:1587587

  10. Discussion and Dissent: ALA 1991 Midwinter Conference Report.

    ERIC Educational Resources Information Center

    Wilson Library Bulletin, 1991

    1991-01-01

    Provides an overview of the American Library Association (ALA) 1991 midwinter conference. Highlights include federal legislation, the organization of library support staff, fees for library services, library collection preservation policies, electronic access, access policy guidelines for academic libraries, reference staffing for electronic…

  11. Losing Libraries, Saving Libraries

    ERIC Educational Resources Information Center

    Miller, Rebecca

    2010-01-01

    This summer, as public libraries continued to get budget hit after budget hit across the country, several readers asked for a comprehensive picture of the ravages of the recession on library service. In partnership with 2010 Movers & Shakers Laura Solomon and Mandy Knapp, Ohio librarians who bought the Losing Libraries domain name, "LJ" launched…

  12. Information 2000. Library and Information Services for the 21st Century. Summary Report of the White House Conference on Library and Information Services (2nd, Washington, D.C., July 9-13, 1991).

    ERIC Educational Resources Information Center

    White House Conference on Library and Information Services.

    This summary report addresses the information needs of American citizens, institutions, schools, industry, and government within the context of three themes: literacy, democracy, and productivity. The report includes a preamble, which provides background information on the conference; discussions of the challenges posed by the Information Age in…

  13. IFLA General Conference 1988. Division of Management and Technology. Section on Information Technology; Section on Statistics; Section on Library Buildings and Equipment; Section on Conservation; Round Table on Management of Library Associations; Round Table on AV Media.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 15 papers in this compilation focus on library nagement and technology, including information technology, statistics, buildings and equipment, and conservation: (1) "Information Control: OSI (Open Systems Interconnection) and Networking Strategies" (Neil McClean, United Kingdom); (2) "OSI in Australia: Potential, Planning, Progress" (Rona…

  14. Digital Debates. WebWise Conference on Libraries and Museums in the Digital World Proceedings (10th, Capitol Hill, Washington, D.C, February 25-27, 2009)

    ERIC Educational Resources Information Center

    Zorich, Diane

    2010-01-01

    Debates typically invoke an image of individuals arguing over the merits of opposing viewpoints. However, the term has a softer, more deliberative sense that connotes reflection, discussion, and consideration. The 2009 WebWise conference, titled "Digital Debates," was conducted in this spirit, with panelists and attendees engaged in complex…

  15. Crossing the Divide: Proceedings of the National Conference of the Association of College and Research Libraries (10th, Denver, Colorado, March 15-18, 2001).

    ERIC Educational Resources Information Center

    Thompson, Hugh A., Ed.

    The Contributed Papers in this volume revolve around seven themes designed to appeal to any librarian exploring new frontiers: Advocacy, Collections, Leadership and Management, Information Literacy, Distance/Distributed Education, Collaboration and Partnerships, and Services for Users. The conference committee received 168 proposals. All were…

  16. Building on the First Century. Proceedings of the National Conference of the Association of College and Research Libraries (5th, Cincinnati, Ohio, April 5-8, 1989).

    ERIC Educational Resources Information Center

    Fennell, Janice C., Ed.

    These proceedings contain 21 research reports and 56 position papers as well as capsule descriptions of 32 workshop forums, panel presentations, and group discussions that took place at the conference. The papers are organized into nine broad subject areas: (1) academic and research librarianship (recruitment, technology and change, faculty…

  17. Navigating the Information Ocean: Charting the Course. Abstracts from the Academic Library Association of Ohio Annual Conference (Columbus, Ohio, November 4, 1994).

    ERIC Educational Resources Information Center

    Academic Library Association of Ohio.

    Abstracts of 14 papers presented at the conference are provided here. Titles are: "Electronic Information Terraforming: Designing and Implementing a Front-end System Using World-Wide Web Technology" (Abbie Basile; And Others); "Characteristics of Generation X and Implications for Reference and Instructional Services" (Catherine A. Lee);…

  18. Online Catalog Screen Displays. A Series of Discussions. Report of a Conference Sponsored by the Council on Library Resources (Austin, Texas, March 10-13, 1985).

    ERIC Educational Resources Information Center

    Williams, Joan Frye, Ed.

    Papers presented and summaries of discussions at a 3-day conference which focused on screen displays for online catalogs are included in this report. Papers presented were: (1) "Suggested Guidelines for Screen Layouts and Design of Online Catalogs" (Joseph R. Matthews); (2) "Displays in Database Search Systems" (Fran Spigai); (3) "Critique of…

  19. Biological & Medical Sciences Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    These two papers on the subjects of videodisc technology and medical library networking were presented at the 1982 International Federation of Library Associations (IFLA) conference. The first, "Videodisc Technology and Applications" by Earl Henderson (United States), examines the videodisc player and the premastering (five-function process: image…

  20. Social Science Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Three papers on the nonconventional literature and social science libraries were presented at the 1983 International Federation of Library Associations (IFLA) conference. In "Grey Material: A Scandinavian View," Birgitta Bergdahl (Sweden) outlines the etymology and meaning of the concept of "grey literature" (which can include reports, theses,…

  1. IATUL Conference 1985.

    ERIC Educational Resources Information Center

    Information Services and Use, 1985

    1985-01-01

    Summarizes presentations at conference on theme "The future of information resources for science and technology and role of libraries": industrial and commercial use of national, regional, and university resources; balance between public- and private-sector resources; local access in national and regional context; access to information in…

  2. Retrospective Conversion of Music Materials. Report of a conference sponsored by the Council on Library Resources (Wayzatta, Minnesota, July 18-19, 1984).

    ERIC Educational Resources Information Center

    Gregor, Dorothy, Ed.

    This meeting on the retrospective conversion (RECON) of music materials brought together 21 individuals to plan the integration and coordination of RECON activities within the context of the music library community. Major music societies and professional music groups were represented as were music faculty and composing communities. This document…

  3. Proceedings of the Conference on Image Storage and Transmission Systems for Libraries...December 1-2, 1969, National Bureau of Standards.

    ERIC Educational Resources Information Center

    Henderson, Madeline M., Comp.

    The challenge of networking is now being extended to cooperative library and information center systems. There are new demands for improved techniques for image storage and transmission, specifically including automated storage and retrieval of microforms and ultra-microforms and new developments in graphic and facsimile transmission. In this…

  4. IFLA Council and General Conference: Conference Programme and Proceedings (65th, Bangkok, Thailand, August 20-28, 1999).

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This proceedings of the 1999 IFLA (International Federation of Library Associations and Institutions) annual conference contains: information on the current, future, and past conferences; a message from H.R.H. Princess Maha Chakri Sirindhorn, the patron of the Thai Library Association; a conference program; an alphabetical list of authors; and 112…

  5. 3rd Annual PIALA Conference Saipan--Collecting, Preserving & Sharing Information in Micronesia. Conference Proceedings. October 13-15, 1993.

    ERIC Educational Resources Information Center

    Edmundson, Margaret, Ed.

    1993-01-01

    This PIALA 1993 Proceedings contains many of the papers presented at the 3rd annual conference of the Pacific Islands Association of Libraries and Archives. This publication is the first time papers from this Micronesian regional library and archives conference have ever been published. The conference addressed various topics of interest to…

  6. Concept, Challenge, Conundrum: From Library Skills to Information Literacy. Proceedings of the National Information Literacy Conference Conducted by the University of South Australia Library and the Australian Library and Information Association Information Literacy Special Interest Group (4th, Adelaide, Australia, December 3-5, 1999).

    ERIC Educational Resources Information Center

    Booker, Di, Ed.

    These proceedings from the fourth National Information Literacy Conference (Decemeber 1999) include the keynote addresses and the papers presented in workshop sessions throughout the conference. Acknowledgements by Irene Doskatsch, conference convener, that precedes the introductory piece by Alan Bundy, "Journey Without End..." is followed by…

  7. PIALA 2000: Libraries and Archives--Where Information and Language Literacy Begin [and] Engaged Readers and Writers in Multicultural Island Communities. Selected Papers from the 10th Pacific Islands Association of Libraries and Archives Conference Joint with the 13th Annual Regional Language Arts Conference (Tumon, Guam, November 9-11, 2000)

    ERIC Educational Resources Information Center

    Cohen, Arlene, Ed.; Quan, Clarisa G., Ed.

    2004-01-01

    This proceedings combines presentations from the jointly held 10th Annual PIALA 2000 Conference and the 13th Annual Regional Language Arts Conference. The volume begins with the welcoming remarks of Mary L. Silk, Christine Ku Scott-Smith, Antonio R. Umpingco, Delia Munoz Rosal, Lawrence Kasperbauer, Rosie Tainatongo, Richard S. Tom, Mary L.…

  8. Oregonians and Their Library and Information Services. A Progress Report.

    ERIC Educational Resources Information Center

    Thorne, Maggie, Comp.

    Compiled in response to questions from Oregon citizens about library and information services in their state, this report contains: (1) a general description of the Governor's Conference on Library and Information Services held in June 1978; (2) a delegate's viewpoint on the White House Conference on Library and Information Services (WHCLIS); (3)…

  9. Libraries and Automation.

    ERIC Educational Resources Information Center

    Markuson, Barbara Evans, Ed.

    The arrangement of this publication follows, as nearly as possible, the actual conference program. The seven sections represent the topics selected by the planning committee as areas of concentration. Each section includes a state-of-the-art paper and related talks and discussion. The topics covered are: the library of the future, file…

  10. School Learners & Libraries.

    ERIC Educational Resources Information Center

    Karlsson, Jenni, Ed.

    This document presents papers from a conference held in November 1995 in Durban, KwaZulu-Natal, convened by the Education Policy Unit (EPU) of the University of Natal as part of research concerning the provision of library-based resources for school learners. Following an introduction are two chapters: "Identifying the Inherited Problems in the…

  11. Creating Library Spaces: Libraries 2040.

    ERIC Educational Resources Information Center

    Bruijnzeels, Rob

    This paper suggests that by 2004, the traditional public libraries will have ceased to exist and new, attractive future libraries will have taken their place. The Libraries 2040 project of the Netherlands is initiating seven different libraries of the future. The Brabant library is the "ultimate library of the future" for the Dutch province of…

  12. IFLA General Conference, 1985. Division on Collections and Services. Section on Acquisition and Exchange. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library material acquisition, collection development, and exchange programs, which were presented at the 1985 International Federation of Library Associations (IFLA) conference, include: (1) "International Collaboration among Acquisition Librarians: Obstacles and Opportunities" (Charles Willett, University of Florida Libraries, United…

  13. Impact of preexisting adenovirus vector immunity on immunogenicity and protection conferred with an adenovirus-based H5N1 influenza vaccine.

    PubMed

    Pandey, Aseem; Singh, Neetu; Vemula, Sai V; Couëtil, Laurent; Katz, Jacqueline M; Donis, Ruben; Sambhara, Suryaprakash; Mittal, Suresh K

    2012-01-01

    The prevalence of preexisting immunity to adenoviruses in the majority of the human population might adversely impact the development of adaptive immune responses against adenovirus vector-based vaccines. To address this issue, we primed BALB/c mice either intranasally (i.n.) or intramuscularly (i.m.) with varying doses of wild type (WT) human adenovirus subtype 5 (HAd5). Following the development of immunity against HAd5, we immunized animals via the i.n. or i.m. route of inoculation with a HAd vector (HAd-HA-NP) expressing the hemagglutinin (HA) and nucleoprotein (NP) of A/Vietnam/1203/04 (H5N1) influenza virus. The immunogenicity and protection results suggest that low levels of vector immunity (<520 virus-neutralization titer) induced by priming mice with up to 10(7) plaque forming units (p.f.u.) of HAd-WT did not adversely impact the protective efficacy of the vaccine. Furthermore, high levels of vector immunity (approximately 1500 virus-neutralization titer) induced by priming mice with 10(8) p.f.u. of HAd-WT were overcome by either increasing the vaccine dose or using alternate routes of vaccination. A further increase in the priming dose to 10(9) p.f.u. allowed only partial protection. These results suggest possible strategies to overcome the variable levels of human immunity against adenoviruses, leading to better utilization of HAd vector-based vaccines.

  14. 75 FR 45647 - National Library of Medicine; Notice of Closed Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ... HUMAN SERVICES National Institutes of Health National Library of Medicine; Notice of Closed Meeting... Committee: National Library of Medicine Special Emphasis Panel, Conflicted Applications. Date: September 22... Library of Medicine, 6705 Rockledge Drive, Suite 301, Bethesda, MD 20817. (Telephone Conference...

  15. A live attenuated Salmonella Enteritidis secreting detoxified heat labile toxin enhances mucosal immunity and confers protection against wild-type challenge in chickens.

    PubMed

    Lalsiamthara, Jonathan; Kamble, Nitin Machindra; Lee, John Hwa

    2016-01-01

    A live attenuated Salmonella Enteritidis (SE) capable of constitutively secreting detoxified double mutant Escherichia coli heat labile toxin (dmLT) was developed. The biologically adjuvanted strain was generated via transformation of a highly immunogenic SE JOL1087 with a plasmid encoding dmLT gene cassette; the resultant strain was designated JOL1641. A balanced-lethal host-vector system stably maintained the plasmid via auxotrophic host complementation with a plasmid encoded aspartate semialdehyde dehydrogenase (asd) gene. Characterization by western blot assay revealed the dmLT subunit proteins in culture supernatants of JOL1641. For the investigation of adjuvanticity and protective efficacy, chickens were immunized via oral or intramuscular routes with PBS, JOL1087 and JOL1641. Birds immunized with JOL1641 showed significant (P ≤ 0.05) increases in intestinal SIgA production at the 1(st) and 2(nd) weeks post-immunization via oral and intramuscular routes, respectively. Interestingly, while both strains showed significant splenic protection via intramuscular immunization, JOL1641 outperformed JOL1087 upon oral immunization. Oral immunization of birds with JOL1641 significantly reduced splenic bacterial counts. The reduction in bacterial counts may be correlated with an adjuvant effect of dmLT that increases SIgA secretion in the intestines of immunized birds. The inclusion of detoxified dmLT in the strain did not cause adverse reactions to birds, nor did it extend the period of bacterial fecal shedding. In conclusion, we report here that dmLT could be biologically incorporated in the secretion system of a live attenuated Salmonella-based vaccine, and that this construction is safe and could enhance mucosal immunity, and protect immunized birds against wild-type challenge. PMID:27262338

  16. State of the Art III: Current Issues of Library Automation

    ERIC Educational Resources Information Center

    Nyren, Karl

    1978-01-01

    Summarizes proceedings of the Library and Information Technology Association's third five-year assessment of the state of the art of library automation. Highlights include both descriptions of presentations and comments from conference participants. (JPF)

  17. The Leishmania infantum Acidic Ribosomal Protein P0 Administered as a DNA Vaccine Confers Protective Immunity to Leishmania major Infection in BALB/c Mice

    PubMed Central

    Iborra, Salvador; Soto, Manuel; Carrión, Javier; Nieto, Ana; Fernández, Edgar; Alonso, Carlos; Requena, Jose M.

    2003-01-01

    In this study, we examined the immunogenic properties of the Leishmania infantum acidic ribosomal protein P0 (LiP0) in the BALB/c mouse model. The humoral and cellular responses induced by the administration of the LiP0 antigen, either as soluble recombinant LiP0 (rLiP0) or as a plasmid DNA formulation (pcDNA3-LiP0), were determined. Also, the immunological response associated with a prime-boost strategy, consisting of immunization with pcDNA3-LiP0 followed by a boost with rLiP0, was assayed. Immunization with rLiP0 induced a predominant Th2-like humoral response, but no anti-LiP0 antibodies were induced after immunization with pcDNA3-LiP0, whereas a strong humoral response consisting of a mixed immunoglobulin G2a (IgG2a)-IgG1 isotype profile was induced in mice immunized with the prime-boost regime. For all three immunization protocols, rLiP0-stimulated production of gamma interferon (IFN-γ) in both splenocytes and lymph node cells from immunized mice was observed. However, it was only when mice were immunized with pcDNA3-LiP0 that noticeable protection against L. major infection was achieved, as determined by both lesion development and parasite burden. Immunization of mice with LiP0-DNA primes both CD4+ and CD8+ T cells, which, with the L. major challenge, were boosted to produce significant levels of IL-12-dependent, antigen-specific IFN-γ. Taken together, these data indicate that genetic vaccination with LiP0 induces protective immunological effector mechanisms, yet the immunological response elicited by LiP0 is not sufficient to keep the infection from progressing. PMID:14573678

  18. Library Theory and Research Section. Education and Research Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library/information science theory and research, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Role of the Library in Computer-Aided Information and Documentation Systems," in which Wolf D. Rauch (West Germany) asserts that libraries must adapt to the increasing use…

  19. Library Buildings and Equipment Section. Management and Technology Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library buildings and equipment, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Impact of Technology on Library Buildings," in which Rolf Fuhlrott (West Germany) discusses construction technology (types of building materials and library building design), selection of…

  20. IFLA General Conference, 1990. Division of Education and Research: Section of Education and Training; Section of Library Theory and Research; Library History, Round Table; Research in Reading, Round Table; Editors of Library Journals, Round Table; and Continuing Professional Education, Round Table; Joint Workshop Papers. Booklet 7.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 21 papers in this collection were presented at the meetings of two sections and three round tables of the Division of Education and Research: (1) "Library and Information Services in Culturally Diverse Communities" (Cheryl Metoyer-Duran); (2) "Library Education in a Pluralistic Society" (Shmuel Sever); (3) "Each One an Individual:…

  1. Primary Babesia rodhaini infection followed by recovery confers protective immunity against B. rodhaini reinfection and Babesia microti challenge infection in mice.

    PubMed

    Wang, Guanbo; Efstratiou, Artemis; Adjou Moumouni, Paul Franck; Liu, Mingming; Jirapattharasate, Charoonluk; Guo, Huanping; Gao, Yang; Cao, Shinuo; Zhou, Mo; Suzuki, Hiroshi; Igarashi, Ikuo; Xuan, Xuenan

    2016-10-01

    In the present study, we investigated the protective immunity against challenge infections with Babesia rodhaini and Babesia microti in the mice recovered from B. rodhaini infection. Six groups with 5 test mice in each group were used in this study, and were intraperitoneally immunized with alive and dead B. rodhaini. The challenge infections with B. rodhaini or B. microti were performed using different time courses. Our results showed that the mice recovered from primary B. rodhaini infection exhibited low parasitemia and no mortalities after the challenge infections, whereas mock mice which had received no primary infection showed a rapid increase of parasitemia and died within 7 days after the challenge with B. rodhaini. Mice immunized with dead B. rodhaini were not protected against either B. rodhaini or B. microti challenge infections, although high titers of antibody response were induced. These results indicate that only mice immunized with alive B. rodhaini could acquire protective immunity against B. rodhaini or B. microti challenge infection. Moreover, the test mice produced high levels of antibody response and low levels of cytokines (INF-γ, IL-4, IL-12, IL-10) against B. rodhaini or B. microti after challenge infection. Mock mice, however, showed rapid increases of these cytokines, which means disordered cytokines secretion occurred during the acute stage of challenge infection. The above results proved that mice immunized with alive B. rodhaini could acquire protective immunity against B. rodhaini and B. microti infections. PMID:27423972

  2. Diuretics Prime Plant Immunity in Arabidopsis thaliana

    PubMed Central

    Noutoshi, Yoshiteru; Ikeda, Mika; Shirasu, Ken

    2012-01-01

    Plant activators are agrochemicals that activate the plant immune system, thereby enhancing disease resistance. Due to their prophylactic and durable effects on a wide spectrum of diseases, plant activators can provide synergistic crop protection when used in combination with traditional pest controls. Although plant activators have achieved great success in wet-rice farming practices in Asia, their use is still limited. To isolate novel plant activators applicable to other crops, we screened a chemical library using a method that can selectively identify immune-priming compounds. Here, we report the isolation and characterization of three diuretics, bumetanide, bendroflumethiazide and clopamide, as immune-priming compounds. These drugs upregulate the immunity-related cell death of Arabidopsis suspension-cultured cells induced with an avirulent strain of Pseudomonas syringae pv. tomato in a concentration-dependent manner. The application of these compounds to Arabidopsis plants confers disease resistance to not only the avirulent but also a virulent strain of the pathogen. Unlike salicylic acid, an endogenous phytohormone that governs disease resistance in response to biotrophic pathogens, the three diuretic compounds analyzed here do not induce PR1 or inhibit plant growth, showing potential as lead compounds in a practical application. PMID:23144763

  3. Immune response

    MedlinePlus

    Innate immunity; Humoral immunity; Cellular immunity; Immunity; Inflammatory response; Acquired (adaptive) immunity ... and usually does not react against them. INNATE IMMUNITY Innate, or nonspecific, immunity is the defense system ...

  4. Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity

    PubMed Central

    Palm, Noah W.; Rosenstein, Rachel K.; Yu, Shuang; Schenten, Dominik; Florsheim, Esther; Medzhitov, Ruslan

    2013-01-01

    SUMMARY Venoms consist of toxic components that are delivered to their victims via bites or stings. Venoms also represent a major class of allergens in humans. Phospholipase A2 (PLA2) is a conserved component of venoms from multiple species and is the major allergen in bee venom. Here we examined how bee venom PLA2 is sensed by the innate immune system and induces a type 2 immune response in mice. We found that bee venom PLA2 induced a T helper type 2 (Th2) cell-type response and group 2 innate lymphoid cell activation via the enzymatic cleavage of membrane phospholipids and release of interleukin-33. Furthermore, we showed that the IgE response to PLA2 could protect mice from future challenge with a near-lethal dose of PLA2. These data suggest that the innate immune system can detect the activity of a conserved component of venoms and induce a protective immune response against a venom toxin. PMID:24210353

  5. Community Immunity (Herd Immunity)

    MedlinePlus

    ... Content Marketing Share this: Main Content Area ​Community Immunity ("Herd" Immunity) Vaccines can prevent outbreaks of disease and save ... disease is contained. This is known as "community immunity." In the illustration below, the top box depicts ...

  6. 99th Dahlem Conference on Infection, Inflammation and Chronic Inflammatory Disorders: Immune therapies of type 1 diabetes: new opportunities based on the hygiene hypothesis

    PubMed Central

    Chatenoud, L; You, S; Okada, H; Kuhn, C; Michaud, B; Bach, J-F

    2010-01-01

    Insulin-dependent (type 1) diabetes is a prototypic organ-specific autoimmune disease resulting from the selective destruction of insulin-secreting β cells within pancreatic islets of Langerhans by an immune-mediated inflammation involving autoreactive CD4+ and CD8+ T lymphocytes which infiltrate pancreatic islets. Current treatment is substitutive, i.e. chronic use of exogenous insulin which, in spite of significant advances, is still associated with major constraints (multiple daily injections, risks of hypoglycaemia) and lack of effectiveness over the long term in preventing severe degenerative complications. Finding a cure for autoimmune diabetes by establishing effective immune-based therapies is a real medical health challenge, as the disease incidence increases steadily in industrialized countries. As the disease affects mainly children and young adults, any candidate immune therapy must therefore be safe and avoid a sustained depression of immune responses with the attendant problems of recurrent infection and drug toxicity. Thus, inducing or restoring immune tolerance to target autoantigens, controlling the pathogenic response while preserving the host reactivity to exogenous/unrelated antigens, appears to be the ideal approach. Our objective is to review the major progress accomplished over the last 20 years towards that aim. In addition, we would like to present another interesting possibility to access new preventive strategies based on the ‘hygiene hypothesis’, which proposes a causal link between the increasing incidence of autoimmune diseases, including diabetes, and the decrease of the infectious burden. The underlying rationale is to identify microbial-derived compounds mediating the protective activity of infections which could be developed therapeutically. PMID:20415859

  7. 99th Dahlem conference on infection, inflammation and chronic inflammatory disorders: immune therapies of type 1 diabetes: new opportunities based on the hygiene hypothesis.

    PubMed

    Chatenoud, L; You, S; Okada, H; Kuhn, C; Michaud, B; Bach, J-F

    2010-04-01

    Insulin-dependent (type 1) diabetes is a prototypic organ-specific autoimmune disease resulting from the selective destruction of insulin-secreting beta cells within pancreatic islets of Langerhans by an immune-mediated inflammation involving autoreactive CD4(+) and CD8(+) T lymphocytes which infiltrate pancreatic islets. Current treatment is substitutive, i.e. chronic use of exogenous insulin which, in spite of significant advances, is still associated with major constraints (multiple daily injections, risks of hypoglycaemia) and lack of effectiveness over the long term in preventing severe degenerative complications. Finding a cure for autoimmune diabetes by establishing effective immune-based therapies is a real medical health challenge, as the disease incidence increases steadily in industrialized countries. As the disease affects mainly children and young adults, any candidate immune therapy must therefore be safe and avoid a sustained depression of immune responses with the attendant problems of recurrent infection and drug toxicity. Thus, inducing or restoring immune tolerance to target autoantigens, controlling the pathogenic response while preserving the host reactivity to exogenous/unrelated antigens, appears to be the ideal approach. Our objective is to review the major progress accomplished over the last 20 years towards that aim. In addition, we would like to present another interesting possibility to access new preventive strategies based on the 'hygiene hypothesis', which proposes a causal link between the increasing incidence of autoimmune diseases, including diabetes, and the decrease of the infectious burden. The underlying rationale is to identify microbial-derived compounds mediating the protective activity of infections which could be developed therapeutically.

  8. An H9N2 influenza virus vaccine prepared from a non-pathogenic isolate from a migratory duck confers protective immunity in mice against challenge with an H9N2 virus isolated from a girl in Hong Kong.

    PubMed

    Nomura, Naoki; Sakoda, Yoshihiro; Soda, Kosuke; Okamatsu, Masatoshi; Kida, Hiroshi

    2012-04-01

    H9N2 influenza viruses circulate in wild birds and poultry in Eurasian countries, and have been isolated from pigs and humans in China. H9N2 viruses isolated from birds, pigs and humans have been classified into three sublineages based on antigenic and genetic features. Chicken antisera to H9N2 viruses of the Korean sublineage reacted with viruses of different sublineages by the hemagglutination-inhibition test. A test vaccine prepared from a non-pathogenic A/duck/Hokkaido/49/1998 (H9N2) strain of the Korean sublineage, obtained from our influenza virus library, induced immunity in mice to reduce the impact of disease caused by the challenge with A/Hong Kong/1073/1999 (H9N2), which is of a different sublineage. The present results indicate that an inactivated whole virus vaccine prepared from a non-pathogenic influenza virus from the library could be used as an emergency vaccine during the early stage of a pandemic caused by H9N2 infection.

  9. Brucella abortusΔcydCΔcydD and ΔcydCΔpurD double-mutants are highly attenuated and confer long-term protective immunity against virulent Brucella abortus.

    PubMed

    Truong, Quang Lam; Cho, Youngjae; Park, Soyeon; Kim, Kiju; Hahn, Tae-Wook

    2016-01-01

    We constructed double deletion (ΔcydCΔcydD and ΔcydCΔpurD) mutants from virulent Brucella abortus biovar 1 field isolate (BA15) by deleting the genes encoding an ATP-binding cassette-type transporter (cydC and cydD genes) and a phosphoribosylamine-glycine ligase (purD). Both BA15ΔcydCΔcydD and BA15ΔcydCΔpurD double-mutants exhibited significant attenuation of virulence when assayed in murine macrophages or in BALB/c mice. Both double-mutants were readily cleared from spleens by 4 weeks post-inoculation even when inoculated at the dose of 10(8) CFU per mouse. Moreover, the inoculated mice showed no splenomegaly, which indicates that the mutants are highly attenuated. Importantly, the attenuation of in vitro and in vivo growth did not impair the ability of these mutants to confer long-term protective immunity in mice against challenge with B. abortus strain 2308. Vaccination of mice with either mutant induced humoral and cell-mediated immune responses, and provided significantly better protection than commercial B. abortus strain RB51 vaccine. These results suggest that highly attenuated BA15ΔcydCΔcydD and BA15ΔcydCΔpurD mutants can be used effectively as potential live vaccine candidates against bovine brucellosis.

  10. Library Schools and Other Training Aspects Section. Education and Research Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library education presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Learning and Library Networks at Mid-Career Level: The Possible Contribution of the Library Schools" by M. H. Heine (United Kingdom); (2) "Network Implications for Library Education: Planning for (the) Future of (the)…

  11. Characterization and Comparison of the Structural Features, Immune-Modulatory and Anti-Avian Influenza Virus Activities Conferred by Three Algal Sulfated Polysaccharides.

    PubMed

    Song, Lin; Chen, Xiaolin; Liu, Xiaodong; Zhang, Fubo; Hu, Linfeng; Yue, Yang; Li, Kecheng; Li, Pengcheng

    2015-12-29

    Three marine macroalgae, i.e., Grateloupia filicina, Ulva pertusa and Sargassum qingdaoense, were selected as the deputies of Rhodophyta, Chlorophyta and Ochrophyta for comparative analysis of the molecular structures and biological activities of sulfated polysaccharides (SP). The ratio of water-soluble polysaccharides, the monosaccharide composition and the sulfated contents of three extracted SPs were determined, and their structures were characterized by Fourier transformation infrared spectroscopy. In addition, biological activity analysis showed that all three SPs had immune-modulatory activity both in vitro and in vivo, and SPs from S. qingdaoense had the best effect. Further bioassays showed that three SPs could not only enhance the immunity level stimulated by inactivated avian influenza virus (AIV) in vivo but also significantly inhibited the activity of activated AIV (H9N2 subtype) in vitro. G. filicina SP exhibited the strongest anti-AIV activity. These results revealed the variations in structural features and bioactivities among three SPs and indicated the potential adjuvants for immune-enhancement and anti-AIV.

  12. Mucosal immunity induced by adenovirus-based H5N1 HPAI vaccine confers protection against a lethal H5N2 avian influenza virus challenge

    SciTech Connect

    Park, Ki Seok; Lee, Jiyeung; Ahn, So Shin; Byun, Young-Ho; Seong, Baik Lin; Baek, Yun Hee; Song, Min-Suk; Choi, Young Ki; Na, Yun Jeong; Hwang, Inhwan; Sung, Young Chul; Lee, Chang Geun

    2009-12-20

    Development of effective vaccines against highly pathogenic avian influenza (HPAI) H5N1 viruses is a global public health priority. Considering the difficulty in predicting HPAI H5N1 pandemic strains, one strategy used in their design includes the development of formulations with the capacity of eliciting broad cross-protective immunity against multiple viral antigens. To this end we constructed a replication-defective recombinant adenovirus-based avian influenza virus vaccine (rAdv-AI) expressing the codon-optimized M2eX-HA-hCD40L and the M1-M2 fusion genes from HPAI H5N1 human isolate. Although there were no significant differences in the systemic immune responses observed between the intramuscular prime-intramuscular boost regimen (IM/IM) and the intranasal prime-intramuscular boost regimen (IN/IM), IN/IM induced more potent CD8{sup +} T cell and antibody responses at mucosal sites than the IM/IM vaccination, resulting in more effective protection against lethal H5N2 avian influenza (AI) virus challenge. These findings suggest that the strategies used to induce multi-antigen-targeted mucosal immunity, such as IN/IM delivery of rAdv-AI, may be a promising approach for developing broad protective vaccines that may be more effective against the new HPAI pandemic strains.

  13. The C-terminus of nisin is important for the ABC transporter NisFEG to confer immunity in Lactococcus lactis.

    PubMed

    AlKhatib, Zainab; Lagedroste, Marcel; Zaschke, Julia; Wagner, Manuel; Abts, André; Fey, Iris; Kleinschrodt, Diana; Smits, Sander H J

    2014-10-01

    The lantibiotic nisin is a small 3.4 kDa antimicrobial peptide, which acts against Gram-positive bacteria in the nmol/L range. Nisin is produced and secreted by several Lactococcus lactis strains to ensure advantages against other bacteria in their habitat. Nisin contains five specific lanthionine rings of which the first two are important for Lipid II binding and the last two are crucial for the pore formation in the membrane. To gain immunity against nisin, the producing strain is expressing an ABC transporter called NisFEG, which expels nisin from the membrane. As a result six to eightfold more nisin is needed to affect the cells. The hydrolysis of ATP by NisFEG is required for this immunity as shown by a mutant, where the ATP hydrolysis is disrupted (NisFH181A EG). Furthermore, NisFEG recognizes the C-terminus of nisin, since deletion of the last six amino acids as well as of the last ring lowered the fold of immunity displayed by NisFEG. PMID:25176038

  14. The C-terminus of nisin is important for the ABC transporter NisFEG to confer immunity in Lactococcus lactis.

    PubMed

    AlKhatib, Zainab; Lagedroste, Marcel; Zaschke, Julia; Wagner, Manuel; Abts, André; Fey, Iris; Kleinschrodt, Diana; Smits, Sander H J

    2014-10-01

    The lantibiotic nisin is a small 3.4 kDa antimicrobial peptide, which acts against Gram-positive bacteria in the nmol/L range. Nisin is produced and secreted by several Lactococcus lactis strains to ensure advantages against other bacteria in their habitat. Nisin contains five specific lanthionine rings of which the first two are important for Lipid II binding and the last two are crucial for the pore formation in the membrane. To gain immunity against nisin, the producing strain is expressing an ABC transporter called NisFEG, which expels nisin from the membrane. As a result six to eightfold more nisin is needed to affect the cells. The hydrolysis of ATP by NisFEG is required for this immunity as shown by a mutant, where the ATP hydrolysis is disrupted (NisFH181A EG). Furthermore, NisFEG recognizes the C-terminus of nisin, since deletion of the last six amino acids as well as of the last ring lowered the fold of immunity displayed by NisFEG.

  15. The C-terminus of nisin is important for the ABC transporter NisFEG to confer immunity in Lactococcus lactis

    PubMed Central

    AlKhatib, Zainab; Lagedroste, Marcel; Zaschke, Julia; Wagner, Manuel; Abts, André; Fey, Iris; Kleinschrodt, Diana; Smits, Sander H J

    2014-01-01

    The lantibiotic nisin is a small 3.4 kDa antimicrobial peptide, which acts against Gram-positive bacteria in the nmol/L range. Nisin is produced and secreted by several Lactococcus lactis strains to ensure advantages against other bacteria in their habitat. Nisin contains five specific lanthionine rings of which the first two are important for Lipid II binding and the last two are crucial for the pore formation in the membrane. To gain immunity against nisin, the producing strain is expressing an ABC transporter called NisFEG, which expels nisin from the membrane. As a result six to eightfold more nisin is needed to affect the cells. The hydrolysis of ATP by NisFEG is required for this immunity as shown by a mutant, where the ATP hydrolysis is disrupted (NisFH181AEG). Furthermore, NisFEG recognizes the C-terminus of nisin, since deletion of the last six amino acids as well as of the last ring lowered the fold of immunity displayed by NisFEG. PMID:25176038

  16. Characterization and Comparison of the Structural Features, Immune-Modulatory and Anti-Avian Influenza Virus Activities Conferred by Three Algal Sulfated Polysaccharides

    PubMed Central

    Song, Lin; Chen, Xiaolin; Liu, Xiaodong; Zhang, Fubo; Hu, Linfeng; Yue, Yang; Li, Kecheng; Li, Pengcheng

    2015-01-01

    Three marine macroalgae, i.e., Grateloupia filicina, Ulva pertusa and Sargassum qingdaoense, were selected as the deputies of Rhodophyta, Chlorophyta and Ochrophyta for comparative analysis of the molecular structures and biological activities of sulfated polysaccharides (SP). The ratio of water-soluble polysaccharides, the monosaccharide composition and the sulfated contents of three extracted SPs were determined, and their structures were characterized by Fourier transformation infrared spectroscopy. In addition, biological activity analysis showed that all three SPs had immune-modulatory activity both in vitro and in vivo, and SPs from S. qingdaoense had the best effect. Further bioassays showed that three SPs could not only enhance the immunity level stimulated by inactivated avian influenza virus (AIV) in vivo but also significantly inhibited the activity of activated AIV (H9N2 subtype) in vitro. G. filicina SP exhibited the strongest anti-AIV activity. These results revealed the variations in structural features and bioactivities among three SPs and indicated the potential adjuvants for immune-enhancement and anti-AIV. PMID:26729137

  17. Antigen delivery by filamentous bacteriophage fd displaying an anti-DEC-205 single-chain variable fragment confers adjuvanticity by triggering a TLR9-mediated immune response.

    PubMed

    Sartorius, Rossella; D'Apice, Luciana; Trovato, Maria; Cuccaro, Fausta; Costa, Valerio; De Leo, Maria Giovanna; Marzullo, Vincenzo Manuel; Biondo, Carmelo; D'Auria, Sabato; De Matteis, Maria Antonietta; Ciccodicola, Alfredo; De Berardinis, Piergiuseppe

    2015-07-01

    Filamentous bacteriophage fd particles delivering antigenic determinants via DEC-205 (fdsc-αDEC) represent a powerful delivery system that induces CD8(+) T-cell responses even when administered in the absence of adjuvants or maturation stimuli for dendritic cells. In order to investigate the mechanisms of this activity, RNA-Sequencing of fd-pulsed dendritic cells was performed. A significant differential expression of genes involved in innate immunity, co-stimulation and cytokine production was observed. In agreement with these findings, we demonstrate that induction of proinflammatory cytokines and type I interferon by fdsc-αDEC was MYD88 mediated and TLR9 dependent. We also found that fdsc-αDEC is delivered into LAMP-1-positive compartments and co-localizes with TLR9. Thus, phage particles containing a single-strand DNA genome rich in CpG motifs delivered via DEC-205 are able to intercept and trigger the active TLR9 innate immune receptor into late endosome/lysosomes and to enhance the immunogenicity of the displayed antigenic determinants. These findings make fd bacteriophage a valuable tool for immunization without administering exogenous adjuvants.

  18. Antigen delivery by filamentous bacteriophage fd displaying an anti-DEC-205 single-chain variable fragment confers adjuvanticity by triggering a TLR9-mediated immune response

    PubMed Central

    Sartorius, Rossella; D'Apice, Luciana; Trovato, Maria; Cuccaro, Fausta; Costa, Valerio; De Leo, Maria Giovanna; Marzullo, Vincenzo Manuel; Biondo, Carmelo; D'Auria, Sabato; De Matteis, Maria Antonietta; Ciccodicola, Alfredo; De Berardinis, Piergiuseppe

    2015-01-01

    Filamentous bacteriophage fd particles delivering antigenic determinants via DEC-205 (fdsc-αDEC) represent a powerful delivery system that induces CD8+ T-cell responses even when administered in the absence of adjuvants or maturation stimuli for dendritic cells. In order to investigate the mechanisms of this activity, RNA-Sequencing of fd-pulsed dendritic cells was performed. A significant differential expression of genes involved in innate immunity, co-stimulation and cytokine production was observed. In agreement with these findings, we demonstrate that induction of proinflammatory cytokines and type I interferon by fdsc-αDEC was MYD88 mediated and TLR9 dependent. We also found that fdsc-αDEC is delivered into LAMP-1-positive compartments and co-localizes with TLR9. Thus, phage particles containing a single-strand DNA genome rich in CpG motifs delivered via DEC-205 are able to intercept and trigger the active TLR9 innate immune receptor into late endosome/lysosomes and to enhance the immunogenicity of the displayed antigenic determinants. These findings make fd bacteriophage a valuable tool for immunization without administering exogenous adjuvants. PMID:25888235

  19. Characterization and Comparison of the Structural Features, Immune-Modulatory and Anti-Avian Influenza Virus Activities Conferred by Three Algal Sulfated Polysaccharides.

    PubMed

    Song, Lin; Chen, Xiaolin; Liu, Xiaodong; Zhang, Fubo; Hu, Linfeng; Yue, Yang; Li, Kecheng; Li, Pengcheng

    2016-01-01

    Three marine macroalgae, i.e., Grateloupia filicina, Ulva pertusa and Sargassum qingdaoense, were selected as the deputies of Rhodophyta, Chlorophyta and Ochrophyta for comparative analysis of the molecular structures and biological activities of sulfated polysaccharides (SP). The ratio of water-soluble polysaccharides, the monosaccharide composition and the sulfated contents of three extracted SPs were determined, and their structures were characterized by Fourier transformation infrared spectroscopy. In addition, biological activity analysis showed that all three SPs had immune-modulatory activity both in vitro and in vivo, and SPs from S. qingdaoense had the best effect. Further bioassays showed that three SPs could not only enhance the immunity level stimulated by inactivated avian influenza virus (AIV) in vivo but also significantly inhibited the activity of activated AIV (H9N2 subtype) in vitro. G. filicina SP exhibited the strongest anti-AIV activity. These results revealed the variations in structural features and bioactivities among three SPs and indicated the potential adjuvants for immune-enhancement and anti-AIV. PMID:26729137

  20. The protoxin Cry1Ac of Bacillus thuringiensis improves the protection conferred by intranasal immunization with Brucella abortus RB51 in a mouse model.

    PubMed

    González-González, Edith; García-Hernández, Ana Lilia; Flores-Mejía, Raúl; López-Santiago, Rubén; Moreno-Fierros, Leticia

    2015-02-25

    Brucellosis is a zoonotic disease affecting many people and animals worldwide. Preventing this infection requires improving vaccination strategies. The protoxin Cry1Ac of Bacillus thuringiensis is an adjuvant that, in addition to increasing the immunogenicity of different antigens, has shown to be protective in different models of parasitic infections. The objective of the present study was to test whether the intranasal co-administration of pCry1Ac with the RB51 vaccine strain of Brucella abortus confers protection against an intranasal challenge with the virulent strain B. abortus 2308 in BALB/c mice. The results showed that co-administration of pCry1Ac and RB51, increased the immunoprotection conferred by the vaccine as evidenced by the following: (1) decrease of the splenic bacterial load when challenged intranasally with the virulent strain; (2) greater in vivo cytotoxic activity in response to the transference of previously infected cells; (3) further proliferation of cytotoxic TCD8+ cells in response to stimulation with heat-inactivated bacteria; (4) increased production of TNF-α and IFN-γ; and (5) significant IgG2a response. These results indicate that the use of the Cry1Ac protein as a mucosal adjuvant via the intranasal route can be a promising alternative for improving current RB51 vaccine against brucellosis. PMID:25497237

  1. The protoxin Cry1Ac of Bacillus thuringiensis improves the protection conferred by intranasal immunization with Brucella abortus RB51 in a mouse model.

    PubMed

    González-González, Edith; García-Hernández, Ana Lilia; Flores-Mejía, Raúl; López-Santiago, Rubén; Moreno-Fierros, Leticia

    2015-02-25

    Brucellosis is a zoonotic disease affecting many people and animals worldwide. Preventing this infection requires improving vaccination strategies. The protoxin Cry1Ac of Bacillus thuringiensis is an adjuvant that, in addition to increasing the immunogenicity of different antigens, has shown to be protective in different models of parasitic infections. The objective of the present study was to test whether the intranasal co-administration of pCry1Ac with the RB51 vaccine strain of Brucella abortus confers protection against an intranasal challenge with the virulent strain B. abortus 2308 in BALB/c mice. The results showed that co-administration of pCry1Ac and RB51, increased the immunoprotection conferred by the vaccine as evidenced by the following: (1) decrease of the splenic bacterial load when challenged intranasally with the virulent strain; (2) greater in vivo cytotoxic activity in response to the transference of previously infected cells; (3) further proliferation of cytotoxic TCD8+ cells in response to stimulation with heat-inactivated bacteria; (4) increased production of TNF-α and IFN-γ; and (5) significant IgG2a response. These results indicate that the use of the Cry1Ac protein as a mucosal adjuvant via the intranasal route can be a promising alternative for improving current RB51 vaccine against brucellosis.

  2. Booster vaccination with safe, modified, live-attenuated mutants of Brucella abortus strain RB51 vaccine confers protective immunity against virulent strains of B. abortus and Brucella canis in BALB/c mice.

    PubMed

    Truong, Quang Lam; Cho, Youngjae; Kim, Kiju; Park, Bo-Kyoung; Hahn, Tae-Wook

    2015-11-01

    Brucella abortus attenuated strain RB51 vaccine (RB51) is widely used in prevention of bovine brucellosis. Although vaccination with this strain has been shown to be effective in conferring protection against bovine brucellosis, RB51 has several drawbacks, including residual virulence for animals and humans. Therefore, a safe and efficacious vaccine is needed to overcome these disadvantages. In this study, we constructed several gene deletion mutants (ΔcydC, ΔcydD and ΔpurD single mutants, and ΔcydCΔcydD and ΔcydCΔpurD double mutants) of RB51 with the aim of increasing the safety of the possible use of these mutants as vaccine candidates. The RB51ΔcydC, RB51ΔcydD, RB51ΔpurD, RB51ΔcydCΔcydD and RB51ΔcydCΔpurD mutants exhibited significant attenuation of virulence when assayed in murine macrophages in vitro or in BALB/c mice. A single intraperitoneal immunization with RB51ΔcydC, RB51ΔcydD, RB51ΔcydCΔcydD or RB51ΔcydCΔpurD mutants was rapidly cleared from mice within 3 weeks, whereas the RB51ΔpurD mutant and RB51 were detectable in spleens until 4 and 7 weeks, respectively. Vaccination with a single dose of RB51 mutants induced lower protective immunity in mice than did parental RB51. However, a booster dose of these mutants provided significant levels of protection in mice against challenge with either the virulent homologous B. abortus strain 2308 or the heterologous Brucella canis strain 26. In addition, these mutants were found to induce a mixed but T-helper-1-biased humoral and cellular immune response in immunized mice. These data suggest that immunization with a booster dose of attenuated RB51 mutants provides an attractive strategy to protect against either bovine or canine brucellosis.

  3. Finding Common Ground: Creating the Library of the Future without Diminishing the Library of the Past. The New Library Series.

    ERIC Educational Resources Information Center

    LaGuardia, Cheryl, Ed.; Mitchell, Barbara A., Ed.

    Adapting today's libraries to better serve tomorrow's needs challenges even the most forward-thinking library staff. This book offers practical, workable solutions from several authorities in the industry. Fifty-five conference papers are included and divided into the following subject areas: "Technology and the Network: Daimons or Demons?";…

  4. National Libraries: An Analysis

    ERIC Educational Resources Information Center

    Burston, Godfrey

    1973-01-01

    The title National Library'' means different things in different countries. It can encompass parliamentary libraries, public libraries, special interest libraries (museums, etc.), and reference and lending libraries. (DH)

  5. Library Roles in American Higher Education

    ERIC Educational Resources Information Center

    Wilson, Logan

    1970-01-01

    This paper, delivered by the president of the American Council on Education at the First U.S. Conference on Libraries and Information Science in Higher Education (Tokyo, Japan, Mary 12, 1969), explores the interrelations between libraries and educational institutions. (JB)

  6. What's New in the Library Automation Arena?

    ERIC Educational Resources Information Center

    Breeding, Marshall

    1998-01-01

    Reviews trends in library automation based on vendors at the 1998 American Library Association Annual Conference. Discusses the major industry trend, a move from host-based computer systems to the new generation of client/server, object-oriented, open systems-based automation. Includes a summary of developments for 26 vendors. (LRW)

  7. Social Science Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on social science documentation and information services presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Problems in the Availability of Some Social Science Publications," a discussion by Maurice B. Line (United Kingdom) of bibliographic control of fugitive literature in the social…

  8. Children's Libraries Section. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on children's services, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "Education and Training of Children's Librarians: An Investigation" by Eva Glistrup (Denmark), which presents preliminary results from a 1982 survey of institutions in 30 countries (30% response rate)…

  9. Geography and Map Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on geography and map librarianship presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Geographical Canada-Research and the Network of Canada Collections in the Federal Republic of Germany" by Hermann Gunzel, which describes the history of German research in Canadian geography, the…

  10. VA Library Service--Today's look at Tomorrow's Library.

    ERIC Educational Resources Information Center

    Veterans Administration, Washington, DC.

    The Conference Poceedings are divided into three broad topics: systems planning, audiovisuals in biomedical communication, and automation and networking. Speakers from within the Veterans Administration (VA), from the National Medical Audiovisual Center, and the Lister Hill National Center for Biomedical Communications, National Library of…

  11. Aberrant apoptotic machinery confers melanoma dual resistance to BRAFV600E inhibitor and immune effector cells: immunosensitization by a histone deacetylase inhibitor

    PubMed Central

    Jazirehi, Ali R; Nazarian, Ramin; Torres-Collado, Antoni Xavier; Economou, James S

    2014-01-01

    BRAFV600E-inhibitors (BRAFi; e.g., vemurafenib) and modern immune-based therapies such as PD-1/PD-L1 and CTLA-4 checkpoints blockade and adoptive cell transfer (ACT) have significantly improved the care of melanoma patients. Having these two effective (BRAFi and immunotherapy) therapies raises the question whether there is a rational biological basis for using them in combination. We developed an in vitro model to determine whether tumor resistance mechanisms to a small molecule inhibitor of a driver oncogene, and to cytotoxic T lymphocyte (CTL)- and natural killer (NK) cell-delivered apoptotic death signals were exclusive or intersecting. We generated melanoma sublines resistant to BRAFi vemurafenib and to CTL recognizing the MART-1 melanoma antigen. Vemurafenib-resistant (VemR) sublines were cross-resistant to MART CTL and NK cells indicating that a common apoptotic pathway governing tumor response to both modalities was disrupted. Pretreatment of VemR melanomas with a histone deacetylase inhibitor (HDACi) restored sensitivity to MART CTL and NK apoptosis by skewing the apoptotic gene programs towards a proapoptotic phenotype. Our in vitro findings suggest that during the course of acquisition of BRAFi resistance, melanomas develop cross-resistance to CTL- and NK-killing. Further, aberrant apoptotic pathways, amenable by an FDA-approved chromatin remodeling drug, regulate tumor resistance mechanisms to immune effector cells. These results may provide rational molecular basis for further investigations to combine these therapies clinically. PMID:24660121

  12. Handling Special Materials in Libraries.

    ERIC Educational Resources Information Center

    Kaiser, Frances E., Ed.

    Libraries of all kinds need and collect special materials for use by their patrons--government publications, technical reports, maps, proprietary publications, company and trade literature, patents and trademarks, standards, symposia and conference records, and related miscellaneous publications. This collection of papers serves as a guide to the…

  13. Discovery of high affinity anti-ricin antibodies by B cell receptor sequencing and by yeast display of combinatorial VH:VL libraries from immunized animals.

    PubMed

    Wang, Bo; Lee, Chang-Han; Johnson, Erik L; Kluwe, Christien A; Cunningham, Josephine C; Tanno, Hidetaka; Crooks, Richard M; Georgiou, George; Ellington, Andrew D

    2016-01-01

    Ricin is a toxin that could potentially be used as a bioweapon. We identified anti-ricin A chain antibodies by sequencing the antibody repertoire from immunized mice and by selecting high affinity antibodies using yeast surface display. These methods led to the isolation of multiple antibodies with high (sub-nanomolar) affinity. Interestingly, the antibodies identified by the 2 independent approaches are from the same clonal lineages, indicating for the first time that yeast surface display can identify native antibodies. The new antibodies represent well-characterized reagents for biodefense diagnostics and therapeutics development.

  14. Discovery of high affinity anti-ricin antibodies by B cell receptor sequencing and by yeast display of combinatorial VH:VL libraries from immunized animals.

    PubMed

    Wang, Bo; Lee, Chang-Han; Johnson, Erik L; Kluwe, Christien A; Cunningham, Josephine C; Tanno, Hidetaka; Crooks, Richard M; Georgiou, George; Ellington, Andrew D

    2016-01-01

    Ricin is a toxin that could potentially be used as a bioweapon. We identified anti-ricin A chain antibodies by sequencing the antibody repertoire from immunized mice and by selecting high affinity antibodies using yeast surface display. These methods led to the isolation of multiple antibodies with high (sub-nanomolar) affinity. Interestingly, the antibodies identified by the 2 independent approaches are from the same clonal lineages, indicating for the first time that yeast surface display can identify native antibodies. The new antibodies represent well-characterized reagents for biodefense diagnostics and therapeutics development. PMID:27224530

  15. Library Skills.

    ERIC Educational Resources Information Center

    Paul, Karin; Kuhlthau, Carol C.; Branch, Jennifer L.; Solowan, Diane Galloway; Case, Roland; Abilock, Debbie; Eisenberg, Michael B.; Koechlin, Carol; Zwaan, Sandi; Hughes, Sandra; Low, Ann; Litch, Margaret; Lowry, Cindy; Irvine, Linda; Stimson, Margaret; Schlarb, Irene; Wilson, Janet; Warriner, Emily; Parsons, Les; Luongo-Orlando, Katherine; Hamilton, Donald

    2003-01-01

    Includes 19 articles that address issues related to library skills and Canadian school libraries. Topics include information literacy; inquiry learning; critical thinking and electronic research; collaborative inquiry; information skills and the Big 6 approach to problem solving; student use of online databases; library skills; Internet accuracy;…

  16. Effect of size and temperature at vaccination on immunization and protection conferred by a live attenuated Francisella noatunensis immersion vaccine in red hybrid tilapia.

    PubMed

    Soto, Esteban; Brown, Nicholas; Gardenfors, Zackarias O; Yount, Shaun; Revan, Floyd; Francis, Stewart; Kearney, Michael T; Camus, Alvin

    2014-12-01

    Francisella noatunensis subsp. orientalis (Fno) is a pleomorphic, facultative intracellular, Gram-negative, emerging bacterial pathogen of marine and fresh water fish with worldwide distribution. In this study, the efficacy of an attenuated Fno intracellular growth locus C (iglC) mutant was evaluated for use as a live immersion vaccine, when administered to hybrid tilapia at two different stages of growth (5 g fry and 10 g fingerlings) and at two temperatures (25 °C and 30 °C). To determine vaccine efficacy, mortality, days to first death, and Fno genome equivalents (GE) in the spleens of survivors, as well as serum and mucus antibody levels, were evaluated after 30 d in fish challenged with a wild type virulent strain. Both size and temperature at vaccination played an important role in immunization and protection. Fry vaccinated at 25 °C were not protected when compared to non-vaccinated fry at 25 °C (p = 0.870). In contrast, 5 g fry vaccinated at 30 °C were significantly protected compared to non-vaccinated fry at 30 °C (p = 0.038). Although lower mortalities occurred, 10 g fingerlings vaccinated at 25 °C were not protected, compared to non-vaccinated fingerlings at 25 °C (p = 0.328), while, 10 g fingerlings vaccinated at 30 °C were significantly protected, compared to non-vaccinated fingerlings at 30 °C (p = 0.038). Additionally, overall mortality of 5 g fish was significantly higher than in 10 g fish. Mortality was also significantly higher in fish subjected to a 30 to 25 °C temperature change one week prior to challenge, than in fish maintained at the same temperature during vaccination and challenge. This information demonstrates that both temperature and size at vaccination are important factors when implementing immunization prophylaxis in cultured tilapia.

  17. Round Table of Editors of Library Journals. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on the publication of library journal literature--from the 1982 International Federation of Library Associations (IFLA) conference--are presented, including: (1) "The Coverage of Library/Information Science Periodicals from Developing Countries by the Major Abstracting and Indexing Services," a report by Barbara J. Buckley on the…

  18. Library Schools: Resource Laboratories for School Media Centers.

    ERIC Educational Resources Information Center

    Main, Linda

    1987-01-01

    Discusses the development of automated management systems for school library media centers. Suggests that schools of library and information science establish microcomputer laboratories containing state-of-the-art library management software and hardware that could be used for field work, inservices, and conferences. (TW)

  19. Tailoring Oversized Courseware to Fit Our Small Library

    ERIC Educational Resources Information Center

    McColl, Lisa; Jones, Jaclyn; Kokolus, Cait

    2004-01-01

    In this article, the author shares her ideas after attending the Computers in Libraries (CIL) Conference in March of 2003. The author is a technical services librarian and system administrator for the Ryan Memorial Library of St. Charles Borromeo Seminary, a library for the Roman Catholic Archdiocese of Philadelphia. She states that many of that…

  20. Oregon State Institutional Library Services; Library Services and Construction Act, Title IV-A's First Thrust - 1968.

    ERIC Educational Resources Information Center

    Gould, Rodney J.

    An evaluation is given of the accomplishments of the State of Oregon institutional libraries between the periods of May 2 and August 23, 1968 including recommendations for future endeavors. Early in 1968 the Assistant State Librarian conferred with institutional administrators and library Advisory Council members for Library Services and…

  1. Trichoderma-induced plant immunity likely involves both hormonal- and camalexin-dependent mechanisms in Arabidopsis thaliana and confers resistance against necrotrophic fungi Botrytis cinerea.

    PubMed

    Contreras-Cornejo, Hexon Angel; Macías-Rodríguez, Lourdes; Beltrán-Peña, Elda; Herrera-Estrella, Alfredo; López-Bucio, José

    2011-10-01

    Filamentous fungi belonging to the genus Trichoderma have long been recognized as agents for the biocontrol of plant diseases. In this work, we investigated the mechanisms involved in the defense responses of Arabidopsis thaliana seedlings elicited by co-culture with Trichoderma virens and Trichoderma atroviride. Interaction of plant roots with fungal mycelium induced growth and defense responses, indicating that both processes are not inherently antagonist. Expression studies of the pathogenesis-related reporter markers pPr1a:uidA and pLox2:uidA in response to T. virens or T. atroviride provided evidence that the defense signaling pathway activated by these fungi involves salicylic acid (SA) and/or jasmonic acid (JA) depending on the amount of conidia inoculated. Moreover, we found that Arabidopsis seedlings colonized by Trichoderma accumulated hydrogen peroxide and camalexin in leaves. When grown under axenic conditions, T. virens produced indole-3-carboxaldehyde (ICAld) a tryptophan-derived compound with activity in plant development. In Arabidopsis seedlings whose roots are in contact with T. virens or T. atroviride, and challenged with Botrytis cinerea in leaves, disease severity was significantly reduced compared to axenically grown seedlings. Our results indicate that the defense responses elicited by Trichoderma in Arabidopsis are complex and involve the canonical defense hormones SA and JA as well as camalexin, which may be important factors in boosting plant immunity.

  2. Intron-hairpin RNA derived from replication associated protein C1 gene confers immunity to tomato yellow leaf curl virus infection in transgenic tomato plants.

    PubMed

    Fuentes, Alejandro; Ramos, Pedro L; Fiallo, Elvira; Callard, Danay; Sánchez, Yadira; Peral, Rudy; Rodríguez, Raidel; Pujol, Merardo

    2006-06-01

    The whitefly-transmitted Tomato Yellow Leaf Curl Virus (TYLCV) is the major pathogen of tomato crop in Cuba and one of the most outstanding viral diseases of plants worldwide. In this work, we have developed transgenic tomato plants, transformed with an intron-hairpin genetic construction to induce post- transcriptional gene silencing against the early TYLCV replication associated protein gene (C1). The intron-hairpin RNA produced involves 726 nts of the 3' end of the TYLCV C1 gene as the arms of the hairpin, and the castor bean catalase intron. Transgenic tomato plants belonging to line 126, which harbor a single transgene copy, showed immunity to TYLCV, even in extreme conditions of infection (4-leaf-stage plants and 300 to many hundreds viruliferous whiteflies per plant during 60 days). Dot blot hybridization of these plants showed no TYLCV DNA presence 60 days after inoculation. Small interfering RNA molecules were detected in both inoculated and non-inoculated plants from line 126. These transgenic tomato plants of the otherwise very TYLCV-susceptible Campbell-28 tomato cultivar, are the first report of resistance to a plant DNA virus obtained by the use of the intron-hairpin RNA approach. PMID:16779645

  3. Immunization of BALB/c mice with Brucella abortus 2308ΔwbkA confers protection against wild-type infection

    PubMed Central

    Li, Zhi-qiang; Gui, Dan; Sun, Zhi-hua; Zhang, Jun-bo; Zhang, Wen-zhi; Guo, Fei

    2015-01-01

    Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308ΔwbkA) and evaluated its virulence. The survival of 2308ΔwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308ΔwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308ΔwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep. PMID:26040616

  4. Novel anti-Tn single-chain Fv-Fc fusion proteins derived from immunized phage library and antibody Fc domain.

    PubMed

    Kubota, Tsuguo; Matsushita, Takefumi; Niwa, Rinpei; Kumagai, Izumi; Nakamura, Kazuyasu

    2010-09-01

    Tn[GalNAc(α1-3)-Ser/Thr] antigen, a tumor-associated carbohydrate antigen, is highly expressed in various tumors and an attractive candidate for cancer immunotherapy. The generation of an anti-Tn antibody is a first step toward the construction of new anticancer molecules. However, because of the simple and small conformation of the Tn molecule, it is difficult to generate an anti-Tn antibody for therapeutic use by conventional hybridoma technology. The purpose of this study was to isolate anti-Tn single-chain antibody fragments (scFv) by phage display technology from a novel immunised library, to attach an antibody constant region (Fc) and to convert them to scFv-Fc fusion proteins. The scFv-Fcs obtained here showed strict specificity against the Tn antigen and also showed antibody-dependent cellular cytotoxicity. These results suggest a potential use of this antibody generating method by phage display and indicate the potential of Fc-fusion proteins as therapeutic candidates.

  5. Marketing & Libraries Do Mix: A Handbook for Libraries and Information Centers.

    ERIC Educational Resources Information Center

    Tenney, H. Baird; And Others

    This handbook offers a practical set of ideas to help all types of libraries in the task of marketing their services in an increasingly competitive economy and provides a model program as urged by the White House Conference on Library and Information Services. It is aimed at adult information services in particular, with passing references to…

  6. Brick and Click Libraries: Proceedings of an Academic Library Symposium (Maryville, Missouri, October 22, 2004)

    ERIC Educational Resources Information Center

    Baudino, Frank, Ed.; Mardis, Lori, Ed.; Park, Sarah G., Ed.; Ury, Connie J., Ed.

    2004-01-01

    The Brick & Click Libraries Symposium is a one-day conference that focuses on providing library resources and services for students who are either on-campus learners or off-campus learners. It is sponsored by Northwest Missouri State University in order to offer academic librarians a forum for sharing practical information. The subjects and…

  7. Library Services to Hospital Patients and Handicapped Readers Section. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library services to hospital personnel, hospital patients, and housebound or handicapped persons, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "Education and Training for Health Care Librarianship," in which Antonia J. Bunch (United Kingdom) discusses the scope of and…

  8. Exome and transcriptome sequencing of Aedes aegypti identifies a locus that confers resistance to Brugia malayi and alters the immune response.

    PubMed

    Juneja, Punita; Ariani, Cristina V; Ho, Yung Shwen; Akorli, Jewelna; Palmer, William J; Pain, Arnab; Jiggins, Francis M

    2015-03-01

    Many mosquito species are naturally polymorphic for their abilities to transmit parasites, a feature which is of great interest for controlling vector-borne disease. Aedes aegypti, the primary vector of dengue and yellow fever and a laboratory model for studying lymphatic filariasis, is genetically variable for its capacity to harbor the filarial nematode Brugia malayi. The genome of Ae. aegypti is large and repetitive, making genome resequencing difficult and expensive. We designed exome captures to target protein-coding regions of the genome, and used association mapping in a wild Kenyan population to identify a single, dominant, sex-linked locus underlying resistance. This falls in a region of the genome where a resistance locus was previously mapped in a line established in 1936, suggesting that this polymorphism has been maintained in the wild for the at least 80 years. We then crossed resistant and susceptible mosquitoes to place both alleles of the gene into a common genetic background, and used RNA-seq to measure the effect of this locus on gene expression. We found evidence for Toll, IMD, and JAK-STAT pathway activity in response to early stages of B. malayi infection when the parasites are beginning to die in the resistant genotype. We also found that resistant mosquitoes express anti-microbial peptides at the time of parasite-killing, and that this expression is suppressed in susceptible mosquitoes. Together, we have found that a single resistance locus leads to a higher immune response in resistant mosquitoes, and we identify genes in this region that may be responsible for this trait. PMID:25815506

  9. Exome and Transcriptome Sequencing of Aedes aegypti Identifies a Locus That Confers Resistance to Brugia malayi and Alters the Immune Response

    PubMed Central

    Juneja, Punita; Ariani, Cristina V.; Ho, Yung Shwen; Akorli, Jewelna; Palmer, William J.; Pain, Arnab; Jiggins, Francis M.

    2015-01-01

    Many mosquito species are naturally polymorphic for their abilities to transmit parasites, a feature which is of great interest for controlling vector-borne disease. Aedes aegypti, the primary vector of dengue and yellow fever and a laboratory model for studying lymphatic filariasis, is genetically variable for its capacity to harbor the filarial nematode Brugia malayi. The genome of Ae. aegypti is large and repetitive, making genome resequencing difficult and expensive. We designed exome captures to target protein-coding regions of the genome, and used association mapping in a wild Kenyan population to identify a single, dominant, sex-linked locus underlying resistance. This falls in a region of the genome where a resistance locus was previously mapped in a line established in 1936, suggesting that this polymorphism has been maintained in the wild for the at least 80 years. We then crossed resistant and susceptible mosquitoes to place both alleles of the gene into a common genetic background, and used RNA-seq to measure the effect of this locus on gene expression. We found evidence for Toll, IMD, and JAK-STAT pathway activity in response to early stages of B. malayi infection when the parasites are beginning to die in the resistant genotype. We also found that resistant mosquitoes express anti-microbial peptides at the time of parasite-killing, and that this expression is suppressed in susceptible mosquitoes. Together, we have found that a single resistance locus leads to a higher immune response in resistant mosquitoes, and we identify genes in this region that may be responsible for this trait. PMID:25815506

  10. 61 FR 58085 - The U.S. National Commission on Libraries and Information Science; Sunshine Act Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    1996-11-12

    ... LIBRARIES AND INFORMATION SCIENCE The U.S. National Commission on Libraries and Information Science... White House Conference on Library and Information Services Taskforce (WHCLIST). MicroSoft Libraries... a.m.-5:30 p.m. December 14, 1996, 9:00 a.m.-1:00 p.m. Dining Room A, Madison Building, Library...

  11. Principles and application of antibody libraries for infectious diseases.

    PubMed

    Lim, Bee Nar; Tye, Gee Jun; Choong, Yee Siew; Ong, Eugene Boon Beng; Ismail, Asma; Lim, Theam Soon

    2014-12-01

    Antibodies have been used efficiently for the treatment and diagnosis of many diseases. Recombinant antibody technology allows the generation of fully human antibodies. Phage display is the gold standard for the production of human antibodies in vitro. To generate monoclonal antibodies by phage display, the generation of antibody libraries is crucial. Antibody libraries are classified according to the source where the antibody gene sequences were obtained. The most useful library for infectious diseases is the immunized library. Immunized libraries would allow better and selective enrichment of antibodies against disease antigens. The antibodies generated from these libraries can be translated for both diagnostic and therapeutic applications. This review focuses on the generation of immunized antibody libraries and the potential applications of the antibodies derived from these libraries.

  12. Competitive Selection from Single Domain Antibody Libraries Allows Isolation of High-Affinity Antihapten Antibodies That Are Not Favored in the llama Immune Response

    PubMed Central

    Rosa, Sofia Tabares-da; Rossotti, Martin; Carleiza, Carmen; Carrión, Federico; Pritsch, Otto; Ahn, Ki Chang; Last, Jerold A.; Hammock, Bruce D; González-Sapienza, Gualberto

    2011-01-01

    Single-domain antibodies (sdAbs) found in camelids, lack a light chain and their antigen-binding site sits completely in the heavy-chain variable domain (VHH). Their simplicity, thermostability, and ease in expression have made VHHs highly attractive. While this has been successfully exploited for macromolecular antigens, their application to the detection of small molecules is still limited to a very few reports, mostly describing low affinity VHHs. Using triclocarban (TCC) as a model hapten, we found that conventional antibodies, IgG1 fraction, reacted with free TCC with a higher relative affinity (IC50 51.0 ng/mL) than did the sdAbs (IgG2 and IgG3, 497 and 370 ng/mL, respectively). A VHH library was prepared, and by elution of phage with limiting concentrations of TCC and competitive selection of binders, we were able to isolate high-affinity clones, KD 0.98–1.37 nM (SPR) which allowed development of a competitive assay for TCC with an IC50 = 3.5 ng/mL (11 nM). This represents a 100-fold improvement with regard to the performance of the sdAb serum fraction, and it is 100-fold better than the IC50 attained with other anti-hapten VHHs reported thus far. Despite the modest overall anti-hapten sdAbs response in llamas, a small subpopulation of high affinity VHHs are generated that can be isolated by carefully design of the selection process. PMID:21827167

  13. Multi-Media and the Changing School Library; A Summary of the Preparations for, Presentations, and Group Reports of the School Library Workshop for Leadership Personnel, Held at the Monte Corona Conference Center, Twin Peaks, Calif., August 6-12, 1967.

    ERIC Educational Resources Information Center

    Brown, James W., Comp; And Others

    The 1967 Monte Corona School Library Workshop for Leadership Personnel, seventh in a series of summer workshops, focuses on school library programs and services; particularly as these relate to a cross-media approach to curriculum implementation. This workshop is designed primarily for school library and audio-visual education leadership personnel…

  14. Libraries program

    USGS Publications Warehouse

    2011-01-01

    The U.S. Congress authorized a library for the U.S. Geological Survey (USGS) in 1879. The library was formally established in 1882 with the naming of the first librarian and began with a staff of three and a collection of 1,400 books. Today, the USGS Libraries Program is one of the world's largest Earth and natural science repositories and a resource of national significance used by researchers and the public worldwide.

  15. IFLA General Conference, 1985. Division on Management and Technology. Section on Conservation. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on conservation and preservation which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Mass Deacidification at the National Library of Canada" (Joyce M. Banks, National Library of Canada); (2) "The National Preservation Office in the British Library" (David W. G. Clements, The…

  16. America's Star Libraries: Top-Rated Libraries

    ERIC Educational Resources Information Center

    Lance, Keith Curry; Lyons, Ray

    2009-01-01

    "Library Journal"'s national rating of public libraries, the "LJ" Index of Public Library Service 2009, Round 2, identifies 258 "star" libraries. Created by Keith Curry Lance and Ray Lyons and based on 2007 data from the IMLS, it rates 7,268 public libraries. The top libraries in each group get five, four, or three stars. All included libraries,…

  17. Museums, Libraries and Cultural Heritage: Democratising Culture, Creating Knowledge and Building Bridges. Adult Learning, Media and Culture. A Series of 29 Booklets Documenting Workshops Held at the Fifth International Conference on Adult Education (Hamburg, Germany, July 14-18, 1997).

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific, and Cultural Organization, Hamburg (Germany). Inst. for Education.

    This booklet examines the role of museums and libraries in providing adult education, both as informal individual learning as well as structured learning activities for groups of learners. The booklet presents an overview of how museums and libraries offer different types of educational opportunities for adults. Most museums have pedagogical…

  18. Changing Dimensions: Managing Library and Information Services for the 1990s: A Global Perspective. Proceedings of the Asian/Pacific American Librarians Association [Conference] (Atlanta, Georgia, July 1, 1991).

    ERIC Educational Resources Information Center

    Sharma, Ravindra Nath, Ed.

    The theme of the 1991 Asian/Pacific American Librarians Association (APALA) was "Changing Dimensions: Managing Library Information Services for the 1990s and Beyond." Members and speakers were invited to discuss this topic from all dimensions of library and information related fields. Presenters had the options either to write on actual issues or…

  19. Proposal for a CLIPS software library

    NASA Technical Reports Server (NTRS)

    Porter, Ken

    1991-01-01

    This paper is a proposal to create a software library for the C Language Integrated Production System (CLIPS) expert system shell developed by NASA. Many innovative ideas for extending CLIPS were presented at the First CLIPS Users Conference, including useful user and database interfaces. CLIPS developers would benefit from a software library of reusable code. The CLIPS Users Group should establish a software library-- a course of action to make that happen is proposed. Open discussion to revise this library concept is essential, since only a group effort is likely to succeed. A response form intended to solicit opinions and support from the CLIPS community is included.

  20. Conference Resolution

    NASA Astrophysics Data System (ADS)

    2009-04-01

    Since the first IUPAP International Conference on Women in Physics (Paris, March 2002) and the Second Conference (Rio de Janeiro, May 2005), progress has continued in most countries and world regions to attract girls to physics and advance women into leadership roles, and many working groups have formed. The Third Conference (Seoul, October 2008), with 283 attendees from 57 countries, was dedicated to celebrating the physics achievements of women throughout the world, networking toward new international collaborations, building each participant's capacity for career success, and aiding the formation of active regional working groups to advance women in physics. Despite the progress, women remain a small minority of the physics community in most countries.

  1. IFLA General Conference, 1991. Workshops' Papers: Section of Geography and Map Libraries; Section of Art Libraries; Section of Children's Libraries joint with RT of Research in Reading; Children's Literature Documentation Centres (RT); Section of Libraries for the Blind joint with Section of Interlending and Document Delivery; Section of Government Information and Official Publications; Section of Information Technology; Professional Board Working Group on Management. Booklet 9.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    The 29 papers in this collection were presented at 9 conference workshops: "Inset Maps and Proposals for Their Description" (V. Kusov); "The Utilization of the Old Maps in Modern Researches" (H. Melnikova); (3) "New Series of Maps for Higher Schools: Their Role in the Cartographic Provision for the Higher Education" (O. Yevteyev); "The…

  2. Networking for Digital Preservation: Current Practice in 15 National Libraries. IFLA Publications 119

    ERIC Educational Resources Information Center

    Verheul, Ingeborg

    2006-01-01

    In 2004-2005, The National Library of the Netherlands (Koninklijke Bibliotheek) conducted a survey for the IFLA-CDNL Alliance for Bibliographic Standards (ICABS)--an alliance founded jointly by the International Federation of Library Associations (IFLA), the Conference of Directors of National Libraries (CDNL) and the national libraries of…

  3. The South Carolina State Library. Tenth Annual Report, July 1, 1978-June 30, 1979.

    ERIC Educational Resources Information Center

    South Carolina State Library, Columbia.

    This annual report reviews state supported library programs and services for FY 1979 and presents a comprehensive summary of the state library system over the past 50 years. A highlight of the year was the South Carolina Governor's Conference on Library and Information Services. Resolutions, debated and enacted, on specific library needs, funding,…

  4. Privatizing Libraries

    ERIC Educational Resources Information Center

    Jerrard, Jane; Bolt, Nancy; Strege, Karen

    2012-01-01

    This timely special report from ALA Editions provides a succinct but comprehensive overview of the "privatization" of public libraries. It provides a history of the trend of local and state governments privatizing public services and assets, and then examines the history of public library privatization right up to the California legislation…

  5. Library Advocacy

    ERIC Educational Resources Information Center

    Plunkett, Kate

    2010-01-01

    This paper is about the issue of advocacy. Standing at the vanguard of literacy, library media specialists have a unique role. However, it is time for media specialists to advocate their services in a proactive way. If library media specialists cannot, both individually and collectively, put advocacy at the forefront, then students will suffer the…

  6. Library Legislation.

    ERIC Educational Resources Information Center

    Kavass, Igor

    Examination of several library legislation models developed to meet the needs of developed and developing nations reveals that our traditional notion of the library's role in society must be abandoned if we wish to reconcile its benefits to its costs. Four models currently exist: many nations, particularly Asian, have no legislation; most nations,…

  7. Biomedical Conferences

    NASA Technical Reports Server (NTRS)

    1976-01-01

    As a result of Biomedical Conferences, Vivo Metric Systems Co. has produced cardiac electrodes based on NASA technology. Frequently in science, one highly specialized discipline is unaware of relevant advances made in other areas. In an attempt to familiarize researchers in a variety of disciplines with medical problems and needs, NASA has sponsored conferences that bring together university scientists, practicing physicians and manufacturers of medical instruments.

  8. Immune System

    MedlinePlus

    ... How Can I Help a Friend Who Cuts? Immune System KidsHealth > For Teens > Immune System Print A A ... could put us out of commission. What the Immune System Does The immune (pronounced: ih-MYOON) system, which ...

  9. [The Presidential Libraries.

    ERIC Educational Resources Information Center

    Webb, John

    There are seven Presidential libraries in various states of existence, from quite active to proposed: (1) Franklin D. Roosevelt Library, (2) Harry S. Truman Library, (3) Herbert Hoover Library, (4) Dwight D. Eisenhower Library, (5) John F. Kennedy Memorial Library (6) Lyndon B. Johnson Library and (7) Rutherford B. Hayes Memorial Library. Each…

  10. Study Provides Insights into Diagnosis, Treatment of Rare Immune Disease: Autoimmmune Lymphoproliferative Syndrome ...

    MedlinePlus

    ... Related Links​ ALPS Unit, Laboratory of Immunology Autoimmune Diseases Immune System Primary Immune Deficiency Diseases National Library of ... Study Provides Insights Into Diagnosis, Treatment of Rare Immune Disease NIH Scientists Report Findings From 20 Years of ...

  11. Computational Biology Support: RECOMB Conference Series (Conference Support)

    SciTech Connect

    Michael Waterman

    2006-06-15

    This funding was support for student and postdoctoral attendance at the Annual Recomb Conference from 2001 to 2005. The RECOMB Conference series was founded in 1997 to provide a scientific forum for theoretical advances in computational biology and their applications in molecular biology and medicine. The conference series aims at attracting research contributions in all areas of computational molecular biology. Typical, but not exclusive, the topics of interest are: Genomics, Molecular sequence analysis, Recognition of genes and regulatory elements, Molecular evolution, Protein structure, Structural genomics, Gene Expression, Gene Networks, Drug Design, Combinatorial libraries, Computational proteomics, and Structural and functional genomics. The origins of the conference came from the mathematical and computational side of the field, and there remains to be a certain focus on computational advances. However, the effective use of computational techniques to biological innovation is also an important aspect of the conference. The conference had a growing number of attendees, topping 300 in recent years and often exceeding 500. The conference program includes between 30 and 40 contributed papers, that are selected by a international program committee with around 30 experts during a rigorous review process rivaling the editorial procedure for top-rate scientific journals. In previous years papers selection has been made from up to 130--200 submissions from well over a dozen countries. 10-page extended abstracts of the contributed papers are collected in a volume published by ACM Press and Springer, and are available at the conference. Full versions of a selection of the papers are published annually in a special issue of the Journal of Computational Biology devoted to the RECOMB Conference. A further point in the program is a lively poster session. From 120-300 posters have been presented each year at RECOMB 2000. One of the highlights of each RECOMB conference is a

  12. Standards for British Libraries.

    ERIC Educational Resources Information Center

    Vaughan, Anthony

    1982-01-01

    Reviews developments in British library standards since 1971, highlighting types of standards, public libraries, academic libraries (university, polytechnic, college), school libraries, and special libraries (hospital and health sciences, prison, subject specializations). Thirty-nine references are cited. (EJS)

  13. Digital Libraries.

    ERIC Educational Resources Information Center

    Fox, Edward A.; Urs, Shalini R.

    2002-01-01

    Provides an overview of digital libraries research, practice, and literature. Highlights include new technologies; redefining roles; historical background; trends; creating digital content, including conversion; metadata; organizing digital resources; services; access; information retrieval; searching; natural language processing; visualization;…

  14. Callpath Library

    2013-11-09

    The "Callpath Library" is a software abstraction layer over a number of stack tracing utilities. It allows tool develoopers to conveniently represent and mNipulate call paths gathered fro U. Wisconsin's Stackwalker API and GNU Backtrace.

  15. Screening and identification of compounds with antiviral activity against hepatitis B virus using a safe compound library and novel real-time immune-absorbance PCR-based high throughput system.

    PubMed

    Lamontagne, Jason; Mills, Courtney; Mao, Richeng; Goddard, Cally; Cai, Dawei; Guo, Haitao; Cuconati, Andy; Block, Timothy; Lu, Xuanyong

    2013-04-01

    There are now seven nucleoside/tide analogues, along with interferon-α, that are approved by the FDA for the management of chronic hepatitis B virus (HBV) infection, a disease affecting hundreds of millions of people worldwide. These medications, however, are limited in usefulness, and significant side effects and the emergence of viral escape mutants make the development of novel and updated therapeutics a pressing need in the treatment of HBV. With this in mind, a library containing 2000 compounds already known to be safe in both humans and mice with known mechanisms of action in mammalian cells were tested for the possibility of either antiviral activity against HBV or selective toxicity in HBV producing cell lines. A modified real-time immune-absorbance-polymerase chain reaction (IA-PCR) assay was developed for this screen, utilizing cells that produce and secrete intact HBV virions. In this procedure, viral particles are first captured by an anti-HBs antibody immobilized on a plate. The viral load is subsequently assessed by real-time PCR directly on captured particles. Using this assay, eight compounds were shown to consistently reduce the amount of secreted HBV viral particles in the culture medium under conditions that had no detectable impact on cell viability. Two compounds, proparacaine and chlorophyllide, were shown to reduce HBV levels 4- to 6-fold with an IC₅₀ of 1 and 1.5 μM, respectively, and were selected for further study. The identification of these compounds as promising antiviral drug candidates against HBV, despite a lack of previous recognition of HBV antiviral activity, supports the validity and utility of testing known compounds for "off-pathogen target" activity against HBV, and also validates this IA-PCR assay as an important tool for the detection of anti-viral activity against enveloped viruses.

  16. IFLA General Conference, 1992. Division of Education and Research: Editors of Library Journals (RT); Section on Research in Reading; Section on Women's Interest in Librarianship; Section on Education and Training; Continuing Professional Education (RT); Section on Library Theory and Research. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    The following 19 papers were delivered at the 1992 annual meeting of the International Federation of Library Associations and Institutions for the Division of Education and Research: (1) "Across the Frontiers: Impact of Foreign Journals in Library Science in India: A Citation Analysis" (M. A. Gopinath); (2) "Children and Reading in Israel" (I.…

  17. California Conference on Networking. Proceedings (Pomona, California, September 19-22, 1985).

    ERIC Educational Resources Information Center

    Johnson, Diane E., Ed.

    This conference was held to bring librarians and users from academic, public, school, and special libraries together to discuss how resource sharing among all types of libraries, for the benefit of all types of users, might be enhanced through formal multitype networking. A conference workbook was provided for participants to read ahead of time,…

  18. Conference Summary

    NASA Technical Reports Server (NTRS)

    Harrington, James L., Jr.

    2000-01-01

    Celebrations and special events were in order this year as the Minority University-Space Interdisciplinary Network (MU-SPIN) Program and NASA's Minority University Research and Education Division (MURED) both reached their 10th anniversaries. In honor of this occasion, the 2000 Annual Users' Conference held at Morris Brown College (MBC) in Atlanta, Georgia, September 11-15, 2000, was the first to be jointly hosted by MU-SPIN and MURED. It was particularly fitting that this anniversary should fall in the year 2000. The start of the new millennium propelled us to push bold new ideas and renew our commitment to minority university participation in all areas of NASA. With the theme 'Celebrating Our Tenth Year With Our Eyes on the Prize,' the conference provided a national forum for showcasing successful MU-SPIN and MURED Program (MUREP) experiences to enhance faculty/student development in areas of scientific and technical research and education. Our NASA-relevant conference agenda resulted in a record-breaking 220 registered attendees. Using feedback from past participants, we designed a track of student activities closely tailored to their interests. The resulting showcase of technical assistance and best practices set a new standard for our conferences in the years to come. This year's poster session was our largest ever, with over 50 presentations from students, faculty, and teachers. Posters covered a broad range of NASA activities from 'A Study of the Spiral Galaxy M101' to 'Network Cabling Characteristics.'

  19. Networking at Conferences: Developing Your Professional Support System

    ERIC Educational Resources Information Center

    Kowalsky, Michelle

    2012-01-01

    The complexity and scale of any large library, education, or technology conference can sometimes be overwhelming. Therefore, spending time reviewing the conference program and perusing the workshop offerings in advance can help you stay organized and make the most of your time at the event. Planning in advance will help you manage potential time…

  20. Does a Border Make a Difference? Library Instruction in the United States and Canada

    ERIC Educational Resources Information Center

    Willingham, Patricia; Carder, Linda; Millson-Martula, Christopher

    2006-01-01

    A brief historical overview of the library instruction scene in the United States and Canada, an analysis of library instruction conference themes, and the identification of perceptions of librarians attending the LOEX, WILU, and LOEX of the West conferences in 2000 and 2001 reveal that the border may be responsible for significant differences…

  1. Conference Proceedings of the International Association of School Librarianship.

    ERIC Educational Resources Information Center

    International Association of School Librarianship, Kalamazoo, MI.

    The 1976 International Association of School Librarianship conference focused on crucial issues in school library development and professional education. Complete texts of the keynote address, Crucial Issues in School Library Development and Professional Education by Frances Henne, and the following presentations are included: The School…

  2. IFLA General Conference, 1985. Division on Regional Activities. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on regional library activities which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Importance of Information Resources in National Development with Particular Reference to the Asian Scene" (Yogendra P. Dubey, India); (2) "Report of the Activities of the Regional Section for Asia…

  3. Energy Information Transfer in Virginia. A Planning Conference.

    ERIC Educational Resources Information Center

    Hickin, Patricia

    The Virginia Planning Conference for Solar Technology Information Transfer was held in Richmond in February 1978 to elicit suggestions on how to make Virginians aware of libraries as sources of energy information and how Virginia libraries can keep their energy materials and reference services up to date. A summary of this planning session and a…

  4. The Crimea 96 Conference: An Orchid of the Future.

    ERIC Educational Resources Information Center

    Spain, Victoria

    1997-01-01

    Describes the conference, "Libraries and Associations in the Transient World: New Technologies and New Forms of Cooperation" (3rd, Foros, Crimea, June 1-9, 1996), which aimed to promote cooperation among libraries in Russia, Ukraine, and other former Soviet Union countries. Sidebars list the English-language papers and the names and origins of the…

  5. America's Star Libraries

    ERIC Educational Resources Information Center

    Lyons, Ray; Lance, Keith Curry

    2009-01-01

    "Library Journal"'s new national rating of public libraries, the "LJ" Index of Public Library Service, identifies 256 "star" libraries. It rates 7,115 public libraries. The top libraries in each group get five, four, or three Michelin guide-like stars. All included libraries, stars or not, can use their scores to learn from their peers and improve…

  6. University Libraries in Pakistan.

    ERIC Educational Resources Information Center

    Haider, Syed Jalaluddin

    1986-01-01

    This profile of university libraries in Pakistan covers history of higher education and role of the library; organization of library service (strong central library, decentralized library service, central library with department libraries); resources and collection building; technical processing; readers' services; administration and staffing;…

  7. Immune response

    MedlinePlus

    ... cells. T cells are responsible for cell-mediated immunity. This type of immunity becomes deficient in persons with HIV, the virus ... blood. B lymphocytes provide the body with humoral immunity as they circulate in the fluids in search ...

  8. Immune Restoration

    MedlinePlus

    ... marrow cells immune to HIV infection. Letting the immune system repair itself: CD4 counts have increased for many ... have taken ART. Some scientists believe that the immune system might be able to heal and repair itself ...

  9. PIALA '94. Pacific Information Liberation: The Wave of the Future. Proceedings of the Annual Pacific Islands Association of Libraries and Archives Conference (4th, Tamuning, Guam, November 4-9, 1994).

    ERIC Educational Resources Information Center

    Cohen, Arlene, Ed.

    Over 100 participants from Micronesia, Australia, Japan, Hawaii, and other United States convened to hear presenters speaking on various topics of interest to librarians, archivists, and educators in the Pacific islands. The proceedings include a welcoming speech, remarks acknowledging conference exhibitors, and a keynote address by Dr. John…

  10. Experimental OAI-Based Digital Library Systems

    NASA Technical Reports Server (NTRS)

    Nelson, Michael L. (Editor); Maly, Kurt (Editor); Zubair, Mohammad (Editor); Rusch-Feja, Diann (Editor)

    2002-01-01

    The objective of Open Archives Initiative (OAI) is to develop a simple, lightweight framework to facilitate the discovery of content in distributed archives (http://www.openarchives.org). The focus of the workshop held at the 5th European Conference on Research and Advanced Technology for Digital Libraries (ECDL 2001) was to bring researchers in the area of digital libraries who are building OAI based systems so as to share their experiences, problems they are facing, and approaches they are taking to address them. The workshop consisted of invited talks from well-established researchers working in building OAI based digital library system along with short paper presentations.

  11. Initial Special Reports Submitted by Member States on the Action Taken by Them Upon the Recommendation Concerning the International Standardization of Library Statistics, Adopted by the General Conference During its Sixteenth Session. Item 21 of the Provisional Agenda.

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific, and Cultural Organization, Paris (France).

    Reports, some only partial, from the member states regarding their action on the topic of international standards for library statistics are presented. The 22 countries represented in this report are: Brazil, Bulgaria, Canada, Cyprus, Cuba, Dahomey, Denmark, Finland, France, Federal Republic of Germany, India, Israel, Japan, Libya, Nepal,…

  12. Collecting, Preserving & Sharing Information in Micronesia. Proceedings of the Annual Pacific Islands Association of Libraries and Archives Conference (3rd, Saipan, Northern Mariana Islands, October 13-15, 1993).

    ERIC Educational Resources Information Center

    Pacific Islands Association of Libraries and Archives, Guam.

    Participants from Washington, Hawaii, Majuro, Palau, Guam and other points in the Northern Mariana Islands came together to share information relating to the functions of libraries and archives as information banks and as preservers of the cultural heritage of Micronesia. Papers presented were: (1) "Reading Motivation in the Pacific" (Marilyn…

  13. PLA 2010 Conference: Plenty for the Price

    ERIC Educational Resources Information Center

    Miller, Rebecca

    2010-01-01

    This article outlines Public Library Association (PLA) 2010 annual conference to be held in Oregon Convention Center, Portland on March 23-27. The days quickly fill up with the picks of over 100 programs as well as valuable "talk tables" and special events. The well-organized schedule, divided into eight broad tracks (e.g., technology,…

  14. Library Bulletin [International Planned Parenthood Federation, August 1973].

    ERIC Educational Resources Information Center

    International Planned Parenthood Federation, London (England).

    Recent additions to the International Planned Parenthood Federation (IPPF) Headquarters Library are listed in this library bulletin. Entries follow the IPPF classification scheme and are arranged by topics: (1) general office management and administration, (2) contraceptive methods, (3) family planning (general), (4) United Nations, conferences,…

  15. Library and Information Policy Perspectives; IFLA Meets in New Delhi.

    ERIC Educational Resources Information Center

    Dyer, Esther

    1992-01-01

    Discusses the highlights of the 58th International Federation of Library Associations and Institutes (IFLA) conference, which was held in New Delhi, India, August 30-September 5, 1992. Outlines IFLA's structure, programs, and goals; introduces new officers; and discusses IFLA's role in strengthening and supporting libraries, particularly in…

  16. Virtual Communities in Europe: The European Library Approach

    ERIC Educational Resources Information Center

    Stigter, Fleur

    2009-01-01

    Purpose: The purpose of this paper is to provide an overview of The Bridging Worlds Conference which was held in October 2008 in Singapore. Design/methodology/approach: One of the main topics the National Library Board of Singapore, the organising party, wished to address was the roles cultural institutions, such as libraries, may play in…

  17. The Library's Contribution to Student Learning: Inspirations and Aspirations

    ERIC Educational Resources Information Center

    Oakleaf, Megan

    2015-01-01

    George Kuh and Robert Gonyea's 2003 article entitled "The Role of the Academic Library in Promoting Student Engagement in Learning" appeared in "College and Research Libraries" in July 2003, after being presented as an invited paper at the ACRL 11th National Conference that April. Herein, Megan Oakleaf describes not only the…

  18. Meeting report: Tenth International Conference on Hantaviruses.

    PubMed

    Papa, Anna; Vaheri, Antti; LeDuc, James W; Krüger, Detlev H; Avšič-Županc, Tatjana; Arikawa, Jiro; Song, Jin-Won; Markotić, Alemka; Clement, Jan; Liang, Mifang; Li, Dexin; Yashina, Liudmila N; Jonsson, Colleen B; Schmaljohn, Connie S

    2016-09-01

    The 10th International Conference on Hantaviruses, organized by the International Society on Hantaviruses, was held from May 31-June 3, 2016 at Colorado State University, Fort Collins, CO, USA. These conferences have been held every three years since 1980. The current report summarizes research presented on all aspects of hantavirology: ecology and epidemiology, virus replication, phylogeny, pathogenesis, immune response, clinical studies, vaccines and therapeutics. PMID:27544703

  19. Brick and Click Libraries: Proceedings of a Regional Academic Library Symposium (Missouri State University, Maryville, Missouri, October 10, 2003)

    ERIC Educational Resources Information Center

    Ury, Connie Jo, Ed.; Baudino, Frank, Ed.

    2003-01-01

    The 2003 Brick and Click is a one-day conference that focuses on providing library resources and services for students who are either on-campus learners or off-campus learners. The conference theme was "The Shape of Tomorrow". It is sponsored by the Northwest Missouri State University in order to offer academic librarians a forum for sharing…

  20. An Examination of the Leadership Program for College Library Directors Associated with ACRL's College Libraries Section

    ERIC Educational Resources Information Center

    Herold, Irene M. H.

    2012-01-01

    The College Libraries Section (CLS) of the Association of College and Research Libraries (ACRL) is the only section specifically for college librarians. In response to a 1989 conference panel of directors' recommendation that there be program for developing college librarians as leaders for the next century, CLS sponsored activities to…

  1. Library Venturing.

    ERIC Educational Resources Information Center

    Wilson, H. Donald

    1986-01-01

    There is opportunity for service and profit to imaginative libraries organizing to provide new forms of knowledge. Librarians as entrepreneurs must learn venture management and finance. Available assistance includes growing entrepreneural understanding in large institutions; family and friends; private wealth-seeking investment; new business…

  2. Library Assessment: The Way We Have Grown

    ERIC Educational Resources Information Center

    Heath, Fred

    2011-01-01

    This essay served as the basis for the opening keynote speech during the Library Assessment Conference in Baltimore in 2010. It set the stage by tracing the demands for accountability in the beginning of the century and outlining progress made. It provided important background and context for the following four keynote papers delivered by Megan…

  3. Gabriel: Gateway to Europe's National Libraries

    ERIC Educational Resources Information Center

    Jefcoate, Graham

    2006-01-01

    Purpose: This paper seeks to look into Gabriel--the Worldwide web server for those European national libraries represented in the Conference of European National Librarians (CENL), providing a single point of access on the internet for the retrieval of information about their functions, services and collections. Above all, it serves as a gateway…

  4. Gone Mobile? (Mobile Libraries Survey 2010)

    ERIC Educational Resources Information Center

    Thomas, Lisa Carlucci

    2010-01-01

    Librarians, like patrons and researchers, are caught between traditional library service models and the promise of evolving information technologies. In recent years, professional conferences have strategically featured programs and presentations geared toward building a mobile agenda and adapting or adopting services to meet new demands of mobile…

  5. Information Literacy for the Skeptical Library Director.

    ERIC Educational Resources Information Center

    Breivik, Patricia S.

    This paper begins by providing background on the information literacy movement, including the educational reform efforts of the 1980s, a higher education summit conference, and the 1989 American Library Association (ALA) Presidential Committee on Information Literacy Final Report. Other highlights include: the information literacy triangle;…

  6. Trends & Issues in Library & Information Science 1990.

    ERIC Educational Resources Information Center

    Eisenberg, Michael B.; And Others

    This synthesis paper provides a state-of-the-art analysis of the field of library and information science based on the results of a content analysis of its literature, including selected journals, conference proceedings, dissertations, and ERIC input. The 14 trends and issues identified by the analysis include developments in technology,…

  7. WebWise 2.0: The Power of Community. WebWise Conference on Libraries and Museums in the Digital World Proceedings (9th, Miami Beach, Florida, March 5-7, 2008)

    ERIC Educational Resources Information Center

    Green, David

    2009-01-01

    Since it was coined by Tim O'Reilly in formulating the first Web 2.0 Conference in 2004, the term "Web 2.0" has definitely caught on as a designation of a second generation of Web design and experience that emphasizes a high degree of interaction with, and among, users. Rather than simply consulting and reading Web pages, the Web 2.0 generation is…

  8. IFLA General Conference, 1984. Management and Technology Division. Section on Statistics. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The three papers in this collection on library statistics were presented at the 1984 IFLA general conference. In "The Management Use of Library Statistics," Geoffrey G. Allen of the Western Australian Institute of Technology discusses current uses and misuses of library statistics and illustrates some practical applications of statistics by citing…

  9. Integrated Immune

    NASA Technical Reports Server (NTRS)

    Crucian, Brian; Mehta, Satish; Stowe, Raymond; Uchakin, Peter; Quiriarte, Heather; Pierson, Duane; Sams, Clarnece

    2010-01-01

    This slide presentation reviews the program to replace several recent studies about astronaut immune systems with one comprehensive study that will include in-flight sampling. The study will address lack of in-flight data to determine the inflight status of immune systems, physiological stress, viral immunity, to determine the clinical risk related to immune dysregulation for exploration class spaceflight, and to determine the appropriate monitoring strategy for spaceflight-associated immune dysfunction, that could be used for the evaluation of countermeasures.

  10. Construction and characterization of a bacterial artificial chromosome library for hexaploid wheat line 92R137

    Technology Transfer Automated Retrieval System (TEKTRAN)

    For map-based cloning of genes conferring important traits in the hexaploid wheat line 92R137, a bacterial artificial chromosome (BAC) library, including two sub libraries, was constructed using the genomic DNA of 92R137 digested with restriction enzymes HindIII and BamHI. The BAC library was compos...

  11. 77 FR 72365 - National Library of Medicine; Notice of Closed Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-05

    ... HUMAN SERVICES National Institutes of Health National Library of Medicine; Notice of Closed Meeting... Committee: National Library of Medicine Special Emphasis Panel; Conflict R01/K99/K22. Date: January 25, 2013... Library of Medicine, 6705 Rockledge Drive, Suite 301, Bethesda, MD 20817, (Telephone Conference...

  12. 78 FR 77475 - National Library of Medicine; Notice of Closed Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-23

    ... HUMAN SERVICES National Institutes of Health National Library of Medicine; Notice of Closed Meeting... Committee: National Library of Medicine Special Emphasis Panel Conflicts R01R21/K01. Date: February 19, 2014... Library of Medicine, 6705 Rockledge Drive, Suite 301, Bethesda, MD 20817, (Telephone Conference...

  13. 76 FR 49779 - National Library of Medicine; Notice of Closed Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-11

    ... HUMAN SERVICES National Institutes of Health National Library of Medicine; Notice of Closed Meeting... Committee: National Library of Medicine Special Emphasis Panel, R01/R13/R21 Conflicteds. Date: September 30... Library of Medicine, 6705 Rockledge Drive, Suite 301, Bethesda, MD 20817, (Telephone Conference...

  14. 78 FR 55265 - National Library of Medicine; Notice of Closed Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-09-10

    ... HUMAN SERVICES National Institutes of Health National Library of Medicine; Notice of Closed Meeting... Committee: National Library of Medicine Special Emphasis Panel; Conflicts R01/K22. Date: October 30, 2013... Library of Medicine, 6705 Rockledge Drive, Suite 301, Bethesda, MD 20817 (Telephone Conference...

  15. Next conference

    NASA Astrophysics Data System (ADS)

    Hexemer, Alexander; Toney, Michael F.

    2010-11-01

    After the successful conference on Synchrotron Radiation in Polymer Science (SRPS) in Rolduc Abbey (the Netherlands), we are now looking forward to the next meeting in this topical series started in 1995 by H G Zachmann, one of the pioneers of the use of synchrotron radiation techniques in polymer science. Earlier meetings were held in Hamburg (1995), Sheffield (2002), Kyoto (2006), and Rolduc (2009). In September of 2012 the Synchrotron Radiation and Polymer Science V conferences will be organized in a joint effort by the SLAC National Accelerator Laboratory and Lawrence Berkeley National Laboratory. Stanford Linear Accelerator Laboratory Stanford Linear Accelerator Laboratory Advanced Light Source at LBL Advanced Light Source at LBL The conference will be organised in the heart of beautiful San Francisco. The program will consist of invited and contributed lectures divided in sessions on the use of synchrotron SAXS/WAXD, imaging and tomography, soft x-rays, x-ray spectroscopy, GISAXS and reflectivity, micro-beams and hyphenated techniques in polymer science. Poster contributions are more than welcome and will be highlighted during the poster sessions. Visits to both SLAC as well as LBL will be organised. San Francisco can easily be reached. It is served by two major international airports San Francisco International Airport and Oakland International Airport. Both are being served by most major airlines with easy connections to Europe and Asia as well as national destinations. Both also boast excellent connections to San Francisco city centre. We are looking forward to seeing you in the vibrant city by the Bay in September 2012. Golden gate bridge Alexander Hexemer Lawrence Berkeley National Laboratory, Advanced Light Source, Berkeley, CA 94720, USA Michael F Toney Stanford Synchrotron Radiation Lightsource, Menlo Pk, CA 94025, USA E-mail: ahexemer@lbl.gov, mftoney@slac.stanford.edu

  16. Conference Summary

    NASA Astrophysics Data System (ADS)

    Ellis, R. S.

    2008-10-01

    This first Subaru international conference has highlighted the remarkably diverse and significant contributions made using the 8.2m Subaru telescope by both Japanese astronomers and the international community. As such, it serves as a satisfying tribute to the pioneering efforts of Professors Keiichi Kodaira and Sadanori Okamura whose insight and dedication is richly rewarded. Here I try to summarize the recent impact of wide field science in extragalactic astronomy and cosmology and take a look forward to the key questions we will address in the near future.

  17. Conferences revisited

    NASA Astrophysics Data System (ADS)

    Radcliffe, Jonathan

    2008-08-01

    Way back in the mid-1990s, as a young PhD student, I wrote a Lateral Thoughts article about my first experience of an academic conference (Physics World 1994 October p80). It was a peach of a trip - most of the lab decamped to Grenoble for a week of great weather, beautiful scenery and, of course, the physics. A whole new community was there for me to see in action, and the internationality of it all helped us to forget about England's non-appearance in the 1994 World Cup finals.

  18. 71 FR 39138 - Notice of Order To Show-Cause; International Air Transport Association Tariff Conference Proceeding

    Federal Register 2010, 2011, 2012, 2013, 2014

    2006-07-11

    ... Conference Proceeding AGENCY: Office of the Secretary, Department of Transportation. SUMMARY: The Department... Provisions for the Conduct of the IATA Traffic Conferences, insofar as that agreement establishes conferences... have immunity from the antitrust laws under 49 U.S.C. 41308 for conference discussions of fares...

  19. 7 CFR 922.67 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 922.67 Section 922.67... COUNTIES IN WASHINGTON Order Regulating Handling Miscellaneous Provisions § 922.67 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart...

  20. 7 CFR 922.67 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 922.67 Section 922.67... COUNTIES IN WASHINGTON Order Regulating Handling Miscellaneous Provisions § 922.67 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart...

  1. 7 CFR 966.87 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 966.87 Section 966.87... Regulating Handling Miscellaneous Provisions § 966.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon the termination of...

  2. 7 CFR 905.85 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 905.85 Section 905.85 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part...

  3. 7 CFR 948.87 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 948.87 Section 948.87... Order Regulating Handling Miscellaneous Provisions § 948.87 Duration of immunities. The benefits, privileges and immunities conferred upon any person by virtue of this subpart shall cease upon...

  4. 7 CFR 925.66 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 925.66 Section 925.66... OF SOUTHEASTERN CALIFORNIA Miscellaneous Provisions § 925.66 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  5. 7 CFR 993.87 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 993.87 Section 993.87... CALIFORNIA Order Regulating Handling Miscellaneous Provisions § 993.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  6. 7 CFR 982.84 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 982.84 Section 982.84... WASHINGTON Order Regulating Handling Miscellaneous Provisions § 982.84 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  7. 7 CFR 932.71 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 932.71 Section 932.71... Regulating Handling Miscellaneous Provisions § 932.71 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon the termination of...

  8. 7 CFR 958.83 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 958.83 Section 958.83 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... § 958.83 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  9. 7 CFR 946.73 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 946.73 Section 946.73... WASHINGTON Order Regulating Handling Miscellaneous Provisions § 946.73 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  10. 7 CFR 915.67 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 915.67 Section 915.67... Order Regulating Handling Miscellaneous Provisions § 915.67 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  11. 7 CFR 923.67 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 923.67 Section 923.67 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  12. 7 CFR 993.87 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 993.87 Section 993.87... CALIFORNIA Order Regulating Handling Miscellaneous Provisions § 993.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  13. 7 CFR 932.71 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 932.71 Section 932.71... Regulating Handling Miscellaneous Provisions § 932.71 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon the termination of...

  14. 7 CFR 905.85 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 905.85 Section 905.85 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part...

  15. 7 CFR 905.85 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 905.85 Section 905.85 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part...

  16. 7 CFR 987.80 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 987.80 Section 987.80 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of...

  17. 7 CFR 945.86 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 945.86 Section 945.86 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... § 945.86 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  18. 7 CFR 985.69 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 985.69 Section 985.69 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing....69 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  19. 7 CFR 948.87 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 948.87 Section 948.87... Order Regulating Handling Miscellaneous Provisions § 948.87 Duration of immunities. The benefits, privileges and immunities conferred upon any person by virtue of this subpart shall cease upon...

  20. 7 CFR 905.85 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 905.85 Section 905.85 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part...

  1. 7 CFR 989.88 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 989.88 Section 989.88... GROWN IN CALIFORNIA Order Regulating Handling Miscellaneous Provisions § 989.88 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this amended...

  2. 7 CFR 959.87 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 959.87 Section 959.87... Regulating Handling Miscellaneous Provisions § 959.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon the termination of...

  3. 7 CFR 917.65 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 917.65 Section 917.65... CALIFORNIA Order Regulating Handling Miscellaneous Provisions § 917.65 Duration of immunities. The benefits, privileges, and immunities conferred by virtue of the provisions of this subpart shall cease upon...

  4. 7 CFR 916.67 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 916.67 Section 916.67... Order Regulating Handling Miscellaneous Provisions § 916.67 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  5. 7 CFR 983.85 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 983.85 Section 983.85..., ARIZONA, AND NEW MEXICO Miscellaneous Provisions § 983.85 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  6. 7 CFR 983.85 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 983.85 Section 983.85..., ARIZONA, AND NEW MEXICO Miscellaneous Provisions § 983.85 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  7. 7 CFR 923.67 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 923.67 Section 923.67 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  8. 7 CFR 906.58 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 906.58 Section 906.58 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  9. 7 CFR 923.67 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 923.67 Section 923.67 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  10. 7 CFR 982.84 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 982.84 Section 982.84... WASHINGTON Order Regulating Handling Miscellaneous Provisions § 982.84 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  11. 7 CFR 915.67 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 915.67 Section 915.67... Order Regulating Handling Miscellaneous Provisions § 915.67 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  12. 7 CFR 923.67 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 923.67 Section 923.67 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  13. 7 CFR 985.69 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 985.69 Section 985.69 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing....69 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  14. 7 CFR 917.65 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 917.65 Section 917.65... CALIFORNIA Order Regulating Handling Miscellaneous Provisions § 917.65 Duration of immunities. The benefits, privileges, and immunities conferred by virtue of the provisions of this subpart shall cease upon...

  15. 7 CFR 956.91 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 956.91 Section 956.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  16. 7 CFR 906.58 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 906.58 Section 906.58 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  17. 7 CFR 946.73 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 946.73 Section 946.73... WASHINGTON Order Regulating Handling Miscellaneous Provisions § 946.73 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  18. 7 CFR 948.87 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 948.87 Section 948.87... Order Regulating Handling Miscellaneous Provisions § 948.87 Duration of immunities. The benefits, privileges and immunities conferred upon any person by virtue of this subpart shall cease upon...

  19. 7 CFR 945.86 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 945.86 Section 945.86 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... § 945.86 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  20. 7 CFR 925.66 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 925.66 Section 925.66... OF SOUTHEASTERN CALIFORNIA Miscellaneous Provisions § 925.66 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  1. 7 CFR 956.91 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 956.91 Section 956.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  2. 7 CFR 922.67 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 922.67 Section 922.67... COUNTIES IN WASHINGTON Order Regulating Handling Miscellaneous Provisions § 922.67 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart...

  3. 7 CFR 993.87 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 993.87 Section 993.87... CALIFORNIA Order Regulating Handling Miscellaneous Provisions § 993.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  4. 7 CFR 983.85 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 983.85 Section 983.85..., ARIZONA, AND NEW MEXICO Miscellaneous Provisions § 983.85 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  5. 7 CFR 959.87 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 959.87 Section 959.87... Regulating Handling Miscellaneous Provisions § 959.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon the termination of...

  6. 7 CFR 946.73 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 946.73 Section 946.73... WASHINGTON Order Regulating Handling Miscellaneous Provisions § 946.73 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  7. 7 CFR 956.91 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 956.91 Section 956.91 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  8. 7 CFR 966.87 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 966.87 Section 966.87... Regulating Handling Miscellaneous Provisions § 966.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon the termination of...

  9. 7 CFR 932.71 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 932.71 Section 932.71... Regulating Handling Miscellaneous Provisions § 932.71 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon the termination of...

  10. 7 CFR 925.66 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 925.66 Section 925.66... OF SOUTHEASTERN CALIFORNIA Miscellaneous Provisions § 925.66 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  11. 7 CFR 945.86 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 945.86 Section 945.86 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... § 945.86 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  12. 7 CFR 923.67 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 923.67 Section 923.67 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  13. 7 CFR 966.87 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 966.87 Section 966.87... Regulating Handling Miscellaneous Provisions § 966.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon the termination of...

  14. 7 CFR 987.80 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 987.80 Section 987.80 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of...

  15. 7 CFR 915.67 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 915.67 Section 915.67... Order Regulating Handling Miscellaneous Provisions § 915.67 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  16. 7 CFR 948.87 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 948.87 Section 948.87... Order Regulating Handling Miscellaneous Provisions § 948.87 Duration of immunities. The benefits, privileges and immunities conferred upon any person by virtue of this subpart shall cease upon...

  17. 7 CFR 989.88 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 989.88 Section 989.88... GROWN IN CALIFORNIA Order Regulating Handling Miscellaneous Provisions § 989.88 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this amended...

  18. 7 CFR 915.67 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 915.67 Section 915.67... Order Regulating Handling Miscellaneous Provisions § 915.67 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  19. 7 CFR 966.87 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 966.87 Section 966.87... Regulating Handling Miscellaneous Provisions § 966.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon the termination of...

  20. 7 CFR 982.84 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 982.84 Section 982.84... WASHINGTON Order Regulating Handling Miscellaneous Provisions § 982.84 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  1. 7 CFR 959.87 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 959.87 Section 959.87... Regulating Handling Miscellaneous Provisions § 959.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon the termination of...

  2. 7 CFR 958.83 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 958.83 Section 958.83 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... § 958.83 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  3. 7 CFR 953.78 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 953.78 Section 953.78... SOUTHEASTERN STATES Order Regulating Handling Miscellaneous Provisions § 953.78 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease...

  4. 7 CFR 985.69 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 985.69 Section 985.69 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING....69 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  5. 7 CFR 917.65 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 917.65 Section 917.65... CALIFORNIA Order Regulating Handling Miscellaneous Provisions § 917.65 Duration of immunities. The benefits, privileges, and immunities conferred by virtue of the provisions of this subpart shall cease upon...

  6. 7 CFR 981.88 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 981.88 Section 981.88... Regulating Handling Miscellaneous Provisions § 981.88 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon its termination except...

  7. 7 CFR 982.84 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 982.84 Section 982.84... WASHINGTON Order Regulating Handling Miscellaneous Provisions § 982.84 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  8. 7 CFR 906.58 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 906.58 Section 906.58 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  9. 7 CFR 917.65 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 917.65 Section 917.65... CALIFORNIA Order Regulating Handling Miscellaneous Provisions § 917.65 Duration of immunities. The benefits, privileges, and immunities conferred by virtue of the provisions of this subpart shall cease upon...

  10. 7 CFR 946.73 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 946.73 Section 946.73... WASHINGTON Order Regulating Handling Miscellaneous Provisions § 946.73 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  11. 7 CFR 985.69 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 985.69 Section 985.69 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing....69 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  12. 7 CFR 948.87 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 8 2011-01-01 2011-01-01 false Duration of immunities. 948.87 Section 948.87... Order Regulating Handling Miscellaneous Provisions § 948.87 Duration of immunities. The benefits, privileges and immunities conferred upon any person by virtue of this subpart shall cease upon...

  13. 7 CFR 985.69 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 985.69 Section 985.69 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING....69 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  14. 7 CFR 953.78 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 953.78 Section 953.78... SOUTHEASTERN STATES Order Regulating Handling Miscellaneous Provisions § 953.78 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease...

  15. 7 CFR 925.66 - Duration of immunities.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 8 2013-01-01 2013-01-01 false Duration of immunities. 925.66 Section 925.66... OF SOUTHEASTERN CALIFORNIA Miscellaneous Provisions § 925.66 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  16. 7 CFR 993.87 - Duration of immunities.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 8 2012-01-01 2012-01-01 false Duration of immunities. 993.87 Section 993.87... CALIFORNIA Order Regulating Handling Miscellaneous Provisions § 993.87 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...

  17. 7 CFR 983.85 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 983.85 Section 983.85..., ARIZONA, AND NEW MEXICO Miscellaneous Provisions § 983.85 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part shall cease upon its...

  18. 7 CFR 906.58 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Duration of immunities. 906.58 Section 906.58 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  19. 7 CFR 945.86 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 945.86 Section 945.86 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (MARKETING... § 945.86 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  20. 7 CFR 982.84 - Duration of immunities.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 8 2014-01-01 2014-01-01 false Duration of immunities. 982.84 Section 982.84... WASHINGTON Order Regulating Handling Miscellaneous Provisions § 982.84 Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this subpart shall cease upon...