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Sample records for library immunization confers

  1. Expression Library Immunization Can Confer Protection against Lethal Challenge with African Swine Fever Virus

    PubMed Central

    Lacasta, Anna; Ballester, María; Monteagudo, Paula L.; Rodríguez, Javier M.; Salas, María L.; Accensi, Francesc; Pina-Pedrero, Sonia; Bensaid, Albert; Argilaguet, Jordi; López-Soria, Sergio; Hutet, Evelyne; Le Potier, Marie Frédérique

    2014-01-01

    ABSTRACT African swine fever is one of the most devastating pig diseases, against which there is no vaccine available. Recent work from our laboratory has demonstrated the protective potential of DNA vaccines encoding three African swine fever viral antigens (p54, p30, and the hemagglutinin extracellular domain) fused to ubiquitin. Partial protection was afforded in the absence of detectable antibodies prior to virus challenge, and survival correlated with the presence of a large number of hemagglutinin-specific CD8+ T cells in blood. Aiming to demonstrate the presence of additional CD8+ T-cell determinants with protective potential, an expression library containing more than 4,000 individual plasmid clones was constructed, each one randomly containing a Sau3AI restriction fragment of the viral genome (p54, p30, and hemagglutinin open reading frames [ORFs] excluded) fused to ubiquitin. Immunization of farm pigs with the expression library yielded 60% protection against lethal challenge with the virulent E75 strain. These results were further confirmed by using specific-pathogen-free pigs after challenging them with 104 hemadsorbing units (HAU) of the cell culture-adapted strain E75CV1. On this occasion, 50% of the vaccinated pigs survived the lethal challenge, and 2 out of the 8 immunized pigs showed no viremia or viral excretion at any time postinfection. In all cases, protection was afforded in the absence of detectable specific antibodies prior to challenge and correlated with the detection of specific T-cell responses at the time of sacrifice. In summary, our results clearly demonstrate the presence of additional protective determinants within the African swine fever virus (ASFV) genome and open up the possibility for their future identification. IMPORTANCE African swine fever is a highly contagious disease of domestic and wild pigs that is endemic in many sub-Saharan countries, where it causes important economic losses and is currently in continuous expansion

  2. IEEE Conference Publications in Libraries.

    ERIC Educational Resources Information Center

    Johnson, Karl E.

    1984-01-01

    Conclusions of surveys (63 libraries, OCLC database, University of Rhode Island users) assessing handling of Institute of Electrical and Electronics Engineers (IEEE) conference publications indicate that most libraries fully catalog these publications using LC cataloging, and library patrons frequently require series access to publications. Eight…

  3. IEEE Conference Publications in Libraries.

    ERIC Educational Resources Information Center

    Johnson, Karl E.

    1984-01-01

    Conclusions of surveys (63 libraries, OCLC database, University of Rhode Island users) assessing handling of Institute of Electrical and Electronics Engineers (IEEE) conference publications indicate that most libraries fully catalog these publications using LC cataloging, and library patrons frequently require series access to publications. Eight…

  4. Reviewing the Electronic Resources & Libraries Conference

    ERIC Educational Resources Information Center

    Tijerina, Bonnie

    2008-01-01

    The third Electronic Resources & Libraries (ER&L) conference gathered at Georgia Institute of Technology's Global Learning and Conference Center in Atlanta, Georgia, March 18-21, 2008. Over 360 attendees, from six countries and from 80% of the United States, represented their libraries and organizations resulting in a diverse and…

  5. Reviewing the Electronic Resources & Libraries Conference

    ERIC Educational Resources Information Center

    Tijerina, Bonnie

    2008-01-01

    The third Electronic Resources & Libraries (ER&L) conference gathered at Georgia Institute of Technology's Global Learning and Conference Center in Atlanta, Georgia, March 18-21, 2008. Over 360 attendees, from six countries and from 80% of the United States, represented their libraries and organizations resulting in a diverse and…

  6. North Carolina Library Association 1997 Biennial Conference.

    ERIC Educational Resources Information Center

    North Carolina Libraries, 1997

    1997-01-01

    Provides summaries of the presentations at the North Carolina Library Association's Biennial Conference, "Choose Quality, Choose Libraries," (Raleigh, NC, October 8-10, 1997). Some of the topics covered include library instruction, Internet access to public documents, outsourcing technical services, copyrights and the Internet, the…

  7. North Carolina Library Association 1997 Biennial Conference.

    ERIC Educational Resources Information Center

    North Carolina Libraries, 1997

    1997-01-01

    Provides summaries of the presentations at the North Carolina Library Association's Biennial Conference, "Choose Quality, Choose Libraries," (Raleigh, NC, October 8-10, 1997). Some of the topics covered include library instruction, Internet access to public documents, outsourcing technical services, copyrights and the Internet, the…

  8. A vaccine prepared from a non-pathogenic H5N1 influenza virus strain from the influenza virus library conferred protective immunity to chickens against the challenge with antigenically drifted highly pathogenic avian influenza virus.

    PubMed

    Samad, Rozanah Asmah Abdul; Nomura, Naoki; Tsuda, Yoshimi; Manzoor, Rashid; Kajihara, Masahiro; Tomabechi, Daisuke; Sasaki, Takashi; Kokumai, Norihide; Ohgitani, Toshiaki; Okamatsu, Masatoshi; Takada, Ayato; Sakoda, Yoshihiro; Kida, Hiroshi

    2011-02-01

    Inactivated influenza virus vaccine prepared from a non-pathogenic influenza virus strain A/duck/Hokkaido/Vac-1/2004 (H5N1) from the virus library conferred protective immunity to chickens against the challenge of antigenically drifted highly pathogenic avian influenza virus (HPAIV), A/whooper swan/Hokkaido/1/2008 (H5N1). The efficacy of the vaccine was comparable to that prepared from genetically modified HPAIV strain deltaRRRRK rg-A/ whooper swan/Mongolia/3/2005 (H5N1), which is more antigenically related to the challenge virus strain, in chickens.

  9. Ohio White House Conference on Library and Information Services: Speak Out. Conference Background Information.

    ERIC Educational Resources Information Center

    Ohio State Library, Columbus.

    Developed as background material for the 1978 Ohio White House Conference on Library and Information Services, this document provides information in six areas of concern for Ohio libraries: (1) library and information services--library users, library collections, special user needs; (2) public awareness--definition, current status, suggestions for…

  10. Institution Library Perspectives. A Report on a Conference on Institution Library Services in Columbus, Ohio.

    ERIC Educational Resources Information Center

    Ohio State Library, Columbus.

    Participants at the 1977 Conference on Institution Library Services reassessed a ten-year old program to develop libraries in Ohio's institutions, reinforced the concept that library services are important to institutions, and elicited input for future development. This report presents the texts of the following conference papers: (1) a report on…

  11. Regional Conference on Libraries (Oklahoma City, Oklahoma, November 3, 1975).

    ERIC Educational Resources Information Center

    Capshaw, Ron, Ed.

    On November 3, 1975, a Regional Conference on Libraries was convened by the Association of Central Oklahoma Governments to make recommendations for library improvement based on perceived local needs. Four workshops had been held before the Conference in Logan, Canadian, Cleveland, and Oklahoma Counties. Recommendations from those workshops were…

  12. IFLA General Conference, 1985. Division of General Research Libraries. Section on National Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on national libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The Lenin State Library in Transition" (N. S. Kartashov, The Lenin State Library, USSR); (2) "Preserving for Access" (Guy Sylvestre, Canadian Institute for Historical Microreproductions,…

  13. IFLA General Conference, 1985. Division of General Research Libraries. Section on National Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on national libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The Lenin State Library in Transition" (N. S. Kartashov, The Lenin State Library, USSR); (2) "Preserving for Access" (Guy Sylvestre, Canadian Institute for Historical Microreproductions,…

  14. Developing Partnerships for California Libraries, Governor's Conference on Libraries (Sacramento, May 26-29, 1968).

    ERIC Educational Resources Information Center

    News Notes of California Libraries, 1968

    1968-01-01

    Representatives from California government, business, industry, and all types of libraries met to discuss interlibrary cooperation and coordination. The addresses presented at the conference and included in these proceedings cover these topics: libraries of the future, major national issues in the development of library networks, library and…

  15. IFLA General Conference, 1986. Libraries Serving the General Public Division. Section: Public Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on public libraries presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Developing Children's Libraries in Shanghai" (Che Jiuzhi, China); (2) "The Development of Public Librarianship in China" (Cheng Fuchen, China); (3) "The Development of Public Libraries in…

  16. IFLA General Conference, 1985. Division on Special Libraries. Section on Biological and Medical Science Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on biological and medical science libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The International Programs of the National Library of Medicine" (Lois Ann Colaianni, United States); (2) "Information Needs for International Health. A CDC (Centers for Disease…

  17. Who Runs Your Library? Proceedings of the 1978 Library Trustees Conference (Montgomery, Alabama).

    ERIC Educational Resources Information Center

    Matthews, M. S., Jr., Ed.

    This report on the second annual conference sponsored by the Alabama Public Library Service (APLS) for public library trustees is an extension of the state's revitalized continuing education for this group, intended to better prepare them to meet their obligations and responsibilities to the libraries and communities they serve. Highlights of this…

  18. IFLA General Conference, 1985. Division on Special Libraries. Section on Biological and Medical Science Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on biological and medical science libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The International Programs of the National Library of Medicine" (Lois Ann Colaianni, United States); (2) "Information Needs for International Health. A CDC (Centers for Disease…

  19. IFLA General Conference, 1986. General Research Libraries Division. Section: National Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on national libraries which were presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Dangers for the Modern Information Society in the Computer Age" (Franz Georg Kaltwasser, West Germany); (2) "Functions and Objectives of the National Libraries in the Developing…

  20. IFLA General Conference, 1985. Division on Special Libraries. Section on Administrative Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on administrative libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Administrative Libraries and Universal Availability of Information: Comments from a Developing Country" (Diekolola Awosika, Nigeria); (2) "Automatic Indexing and the Dissemination of…

  1. IFLA General Conference, 1984. General Research Libraries Division. Section on National Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on national library services and activities which were presented at the 1984 IFLA general conference, include: (1) "The Role of National Libraries of the Soviet Union in Reading Promotion" (N. S. Kartashov, USSR); (2) "Regional Library Cooperation in East and Southeast Asia--A Japanese Overview" (Takayasu Miyakawa,…

  2. IFLA General Conference, 1986. Libraries Serving the General Public Division. Section: Libraries for the Blind. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on libraries for the blind presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Report on 'Study on the Tools to Improve the Availability of Library Materials for the Blind'" (Winnie Vitzansky, Denmark); (2) "Planning the Third Edition of 'The International Directory of…

  3. IFLA General Conference, 1985. Division on Special Libraries. Section on Administrative Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on administrative libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Administrative Libraries and Universal Availability of Information: Comments from a Developing Country" (Diekolola Awosika, Nigeria); (2) "Automatic Indexing and the Dissemination of…

  4. IFLA General Conference, 1986. Special Libraries Division. Section: Biological and Medical Sciences Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Four papers on biological and medical sciences libraries were presented at the 1986 International Federation of Library Associations (IFLA) conference. "Activities and Services of Medical Libraries in Japan--Past, Present, and Future" (Kazuo Urata and Toshinobu Suga, Japan) discusses the inauguration of the Japan Medical Library…

  5. IFLA General Conference, 1985. Division of General Research Libraries. Section on Parliamentary Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on parliamentary libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Cooperation among Parliamentary Libraries--Example: Federal Republic of Germany" (Wolfgang Dietz, West Germany); (2) "Cooperative Development in Action: Towards an Australian Parliamentary Information…

  6. IFLA General Conference, 1986. General Research Libraries Division. Section: National Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on national libraries which were presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Dangers for the Modern Information Society in the Computer Age" (Franz Georg Kaltwasser, West Germany); (2) "Functions and Objectives of the National Libraries in the Developing…

  7. IFLA General Conference, 1984. General Research Libraries Division. Section on Parliamentary Libraries; Section on Public Libraries; Section on University and Other General Research Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on government libraries, public libraries, and research libraries presented at the 1984 IFLA general conference include: (1) "Library Services for Research" (Maria S. Pla de Menendez, Colombia); (2) "Interlibrary Loans, Present and Future: A Consideration for Academic Library Management" (Geoffrey G. Allen, Australia);…

  8. IFLA General Conference, 1984. General Research Libraries Division. Section on Parliamentary Libraries; Section on Public Libraries; Section on University and Other General Research Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on government libraries, public libraries, and research libraries presented at the 1984 IFLA general conference include: (1) "Library Services for Research" (Maria S. Pla de Menendez, Colombia); (2) "Interlibrary Loans, Present and Future: A Consideration for Academic Library Management" (Geoffrey G. Allen, Australia);…

  9. Libraries in Society. Proceedings of the Biennial Conference of the Library Association of Australia (19th, Tasmania, Australia, August 1977).

    ERIC Educational Resources Information Center

    Library Association of Australia, Sidney.

    This collection of 34 papers and speeches presented at the conference covers a wide range of topics pertaining to the role of libraries in contemporary society, including issues in library education, library/community relations, library planning and policy-making, technological trends, library services, and library administration. While several…

  10. International Federation of Library Associations Annual Conference Papers. General Research Libraries Division: Parliamentary Libraries and National Libraries Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Gude, Gilbert; And Others

    This set of papers presented to the General Research Libraries Division of the International Federation of Library Associations (IFLA) during its 47th annual conference (1981) includes: "The Effect of the Introduction of Computers on Library and Research Staff," by Gilbert Gude; "Libraries as Information Service Agencies…

  11. International Federation of Library Associations Annual Conference Papers. General Research Libraries Division: Parliamentary Libraries and National Libraries Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Gude, Gilbert; And Others

    This set of papers presented to the General Research Libraries Division of the International Federation of Library Associations (IFLA) during its 47th annual conference (1981) includes: "The Effect of the Introduction of Computers on Library and Research Staff," by Gilbert Gude; "Libraries as Information Service Agencies…

  12. IFLA General Conference, 1985. Division on Special Libraries. Section on Art Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on art libraries and art documentation which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The Universal Availability of Art Publications: A Global Context" (Philip Pacey, United Kingdom); (2) "Art Librarianship in Norway" (Anne Lise Rabben and Cecilie W.…

  13. IFLA General Conference, 1984. Special Libraries Division. Section on Social Science Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The two papers in this document on social science libraries were presented at the 1984 IFLA general conference. In "Library and Continuing Education with Implications for Developing Countries: A Research Essay," David R. Bender (United States) examines factors impacting upon the skills necessary for effective librarianship in the social…

  14. IFLA General Conference, 1985. Division on Special Libraries. Section on Art Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on art libraries and art documentation which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The Universal Availability of Art Publications: A Global Context" (Philip Pacey, United Kingdom); (2) "Art Librarianship in Norway" (Anne Lise Rabben and Cecilie W.…

  15. IFLA General Conference, 1985. Division on Special Libraries. Section on Science and Technology Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on science and technology libraries which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "UAP (Universal Availability of Publications) and User Training for Categories of Grey Literature" (Dieter Schmidmaier, Mining Academy Freiberg, East Germany); (2) "Resource…

  16. IFLA General Conference, 1986. Special Libraries Division. Section: Geography and Map Library. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Four papers on geography and map libraries were presented at the 1986 International Federation of Library Associations (IFLA) conference. "Generation and Utilization of Maps and Atlases in Japan," by Takashi Morita of Japan, presents an overview of the making and uses of maps and atlases in Japan and concludes that a comprehensive…

  17. LISA-The Library and Information Services in Astronomy Conferences

    DTIC Science & Technology

    2007-01-01

    Introduction The world of astronomy is small. Given the fact that astronomy is dealing with the universe , its structure and composition, this may sound...major US universities and all library and information science schools, making the LISA I proceedings the most widely distributed of any LISA conference...to university librarians, affiliated with large library systems. The time and site of LISA I were excellently chosen to assure attendance by a large

  18. IFLA General Conference, 1985. Division on Special Libraries. Section on Social Science Libraries and Geography and Map Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers presented on social science and map and geography libraries at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Information for the Developing World: NTIS's (National Technical Information Service) Role in Information Transfer to Developing Countries" (Joseph F. Caponio, United States); (2)…

  19. IFLA General Conference, 1985. Division on Special Libraries. Section on Social Science Libraries and Geography and Map Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers presented on social science and map and geography libraries at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Information for the Developing World: NTIS's (National Technical Information Service) Role in Information Transfer to Developing Countries" (Joseph F. Caponio, United States); (2)…

  20. IFLA General Conference, 1986. Education and Research Division. Section: Library History and Library Theory and Research. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on library history and library theory and research presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "The History of Western Literature Library Collections in Japan" (Yoshitomi Okazaki, Japan); (2) "Trends of Library History Research in Japan" (Hiroshi Kawai, Yukio…

  1. IFLA General Conference, 1986. Education and Research Division. Section: Library History and Library Theory and Research. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on library history and library theory and research presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "The History of Western Literature Library Collections in Japan" (Yoshitomi Okazaki, Japan); (2) "Trends of Library History Research in Japan" (Hiroshi Kawai, Yukio…

  2. The Conference on the Future of the Public Library.

    ERIC Educational Resources Information Center

    Sager, Donald

    1988-01-01

    Summarizes events at an OCLC-sponsored conference at which three major issues were addressed: (1) how public libraries will improve access to services and resources; (2) how public librarians will interact with users and what skills/attitudes they will require; and (3) the impact of interlibrary cooperation in light of changes in society and…

  3. Libraries: Key to Democracy. The Governor's Conference on the Future of Libraries (Las Vegas, Nevada, May 9-11, 1990).

    ERIC Educational Resources Information Center

    Nevada State Library and Archives, Carson City.

    This report discusses the recent Governor's Conference, as well as the activities of Nevada's library community in addressing the national conference theme of literacy, productivity, and democracy in library and information services. Briefly highlighted are the discussion papers presented at the conference; the forums that addressed key library…

  4. Immune Antibody Libraries: Manipulating The Diverse Immune Repertoire for Antibody Discovery.

    PubMed

    Lim, Theam Soon; Chan, Soo Khim

    2016-01-01

    Antibody phage display is highly dependent on the availability of antibody libraries. There are several forms of libraries depending mainly on the origin of the source materials. There are three major classes of libraries, mainly the naïve, immune and synthetic libraries. Immune antibody libraries are designed to isolate specific and high affinity antibodies against disease antigens. The pre-exposure of the host to an infection results in the production of a skewed population of antibodies against the particular infection. This characteristic takes advantage of the in vivo editing machinery to generate bias and specific immune repertoire. The skewed but diverse repertoire of immune libraries has been adapted successfully in the generation of antibodies against a wide range of diseases. We envisage immune antibody libraries to play a greater role in the discovery of antibodies for diseases in the near future. Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.

  5. National Conference on Library Statistics (Chicago, June 6-8, 1966).

    ERIC Educational Resources Information Center

    Trezza, Alphonse F., Ed.; Beasley, James, Ed.

    Attended by representatives of American Library Association (ALA) divisions and committees, other national library associations, state library agencies, library schools, and all types of libraries, this conference was held to discuss: (1) the program of the National Center for Educational Statistics (USOE) as it pertains to libraries; (2) the…

  6. IFLA General Conference, 1985. Division of Libraries Serving the General Public. Section on Libraries for the Blind. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library services and programs for the blind and visually handicapped which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "Copyright and Library Material for the Blind" (Elizabeth Twining, United Kingdom); (2) "Postal and Customs Regulations and Library Material…

  7. Libraries in Transition: Responses to Change. Selected Papers from North Central Library Conference (Milwaukee, October 11-14, 1967).

    ERIC Educational Resources Information Center

    Schwab, Bernard, Comp.

    This report includes selected papers and edited discussions recorded during the North Central Library Conference of 1967. Topics discussed include: changing perspectives in librarianship; legislation and changing library needs; library personnel needs, recruitment, and training problems; library cooperation; research in reference service; and…

  8. Brick & Click Libraries: An Academic Library Conference Proceedings (16th, Maryville, Missouri, November 4, 2016)

    ERIC Educational Resources Information Center

    Baudino, Frank, Ed.; Johnson, Carolyn, Ed.

    2016-01-01

    Twenty scholarly papers and fifteen abstracts comprise the content of the sixteenth annual Brick and Click Libraries Conference, held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship. The…

  9. IFLA General Conference, 1984. Special Libraries Division. Section on Geography and Map Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    This document comprises two papers on geography and map libraries and cartographic materials which were presented at the 1984 IFLA general conference. "Cartographic Materials as a Resource for National and Regional Planning and Development," by E. Hans van de Waal (Utrecht), addresses the importance of cartographic materials for…

  10. Brick & Click Libraries: An Academic Library Conference (15th, Maryville, Missouri, November 6, 2015)

    ERIC Educational Resources Information Center

    Baudino, Frank, Ed.; Johnson, Carolyn, Ed.

    2015-01-01

    Nineteen scholarly papers and seventeen abstracts comprise the content of the fifteenth annual Brick and Click Libraries Conference, held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship.…

  11. IFLA General Conference, 1985. Division on Education and Research. Section: Round Table on Library History. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library history presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The Historical Role of Libraries in Scholarly Communication" (Pamela Spence Richards, Rutgers University, United States); (2) "Current Bibliographies for Library History: An International Review and a Feasibility Study"…

  12. The White House Conference on School Libraries Proceedings (Washington, DC, June 4, 2002).

    ERIC Educational Resources Information Center

    Institute of Museum and Library Services, Washington, DC.

    Laura Bush hosted a White House Conference on School Libraries on June 4, 2002, to discuss the latest research on libraries, student achievement and successful local programs. Mrs. Bush's cohost was Dr. Robert Martin, Director of the Institute of Museum and Library Services and education, library, government and philanthropic leaders from across…

  13. IFLA General Conference, 1985. Division on Education and Research. Section: Round Table on Library History. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library history presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "The Historical Role of Libraries in Scholarly Communication" (Pamela Spence Richards, Rutgers University, United States); (2) "Current Bibliographies for Library History: An International Review and a Feasibility Study"…

  14. CONFERENCE ON JUNIOR COLLEGE LIBRARIES (2ND, NORTHERN ILLINOIS UNIVERSITY, DEKALB, APRIL 28-29, 1967).

    ERIC Educational Resources Information Center

    Illinois Library Association, Chicago.

    AT THE SECOND ANNUAL ILLINOIS CONFERENCE ON JUNIOR COLLEGE LIBRARIES, SIX PAPERS WERE PRESENTED. THE FIRST COVERED THE NATURE OF LIBRARY SERVICES TO STUDENTS, FACULTY, AND PUBLIC. A SECOND SPEAKER DESCRIBED THE DEVELOPMENT OF THE ILLINOIS JUNIOR COLLEGE LIBRARIES, NOTING THAT THEY DID NOT GENERALLY MEET THE AMERICAN LIBRARY ASSOCIATION STANDARDS…

  15. [Library and Information Services for Democracy, for Literacy, and for Productivity]. Conference Briefs.

    ERIC Educational Resources Information Center

    Bobinski, George, Ed.

    1990-01-01

    This issue is designed to provide delegates and other participants in the New York State Governor's Conference in 1990 and the White House Conference on Library and Information Services in July 1991 with an overview of the current status and future needs of libraries and librarianship in the state. Following a message from Mario Cuomo, Governor of…

  16. [Library and Information Services for Democracy, for Literacy, and for Productivity]. Conference Briefs.

    ERIC Educational Resources Information Center

    Bobinski, George, Ed.

    1990-01-01

    This issue is designed to provide delegates and other participants in the New York State Governor's Conference in 1990 and the White House Conference on Library and Information Services in July 1991 with an overview of the current status and future needs of libraries and librarianship in the state. Following a message from Mario Cuomo, Governor of…

  17. IFLA General Conference, 1986. Libraries Serving the General Public Division. Section: Library Services to Multicultural/Multilingual Populations. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers on library services to multicultural and multilingual populations presented at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "The Use of New Technologies to Foster Literacy among Minorities" (Shmuel Sever, Israel); (2) "Information Services for Culturally Suppressed Groups"…

  18. Libraries and National Development (Final Report of the Third Afro-Nordic Library Conference, Finland, September 3-7 1979).

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific, and Cultural Organization, Paris (France).

    Contained in this collection of presentations from the third Afro-Nordic Library Conference are a foreword describing the objectives of the meeting and 14 papers: "From Oral Tradition to Literary Writing: The Awakening of National Awareness," by Heikki Kirkinen; "African Library Systems," by L. E. Samarasinghe; "Planning…

  19. IFLA General Conference, 1984. Special Libraries Division. Section on Art Libraries; Section on Music Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The five papers in this collection on art and music environments in developing countries were presented at the 1984 IFLA general conference. (1) In "Developing an Art for Life's Sake," Gavin Jantjes, a black South African artist currently living and working in the United Kingdom, offers personal viewpoints on issues relating to the art…

  20. IFLA General Conference, 1984. Special Libraries Division. Section on Art Libraries; Section on Music Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The five papers in this collection on art and music environments in developing countries were presented at the 1984 IFLA general conference. (1) In "Developing an Art for Life's Sake," Gavin Jantjes, a black South African artist currently living and working in the United Kingdom, offers personal viewpoints on issues relating to the art…

  1. IFLA General Conference, 1986. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This document includes papers on libraries serving the general public which were presented at the 1986 International Federation of Library Associations (IFLA) conference. The first paper, "Annual Reports of Sections and Round Tables of the Division of Libraries Serving the General Public" includes the following annual reports for fiscal year…

  2. Computers in Libraries Annual Conference (17th, Washington, DC, March 13-15, 2002): Collected Presentations.

    ERIC Educational Resources Information Center

    Nixon, Carol, Comp.

    This book contains presentations from the 17th annual Computers in Libraries Conference. Topics covered include: chatting with a librarian; verbots for library Web sites; collaborative IT (Information Technology) planning at Montgomery County Public Library (Maryland); designing a local government taxonomy; Weblogs; new roles for librarians in…

  3. Guam Governor's Conference on Library and Information Services, 1990. Final Report.

    ERIC Educational Resources Information Center

    Wang, Chih; And Others

    This is the final report of the Guam Governor's Conference on Library and Information Services (GGCLIS), 1990. Approximately 160 delegates, representing library and information professionals, active library supporters, territorial and federal government officials, and the reading public, gathered together to develop recommendations for the…

  4. IFLA General Conference, 1986. Education and Research Division. Section: Library School and Other Training Aspects. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This document includes four papers on library school and other training aspects which were presented at the 1986 conference of the International Federation of Library Associations (IFLA). In "Meeting the Challenge of the High Tech Information Age--The Recent Development of Library and Information Science Education in the People's Republic of…

  5. IFLA General Conference, 1986. Education and Research Division. Section: Library School and Other Training Aspects. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This document includes four papers on library school and other training aspects which were presented at the 1986 conference of the International Federation of Library Associations (IFLA). In "Meeting the Challenge of the High Tech Information Age--The Recent Development of Library and Information Science Education in the People's Republic of…

  6. IFLA General Conference, 1986. Management and Technology Division. Section: Management of Library Associations (RT). Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    Papers presented at a session on management of library associations at the 1986 International Federation of Library Associations (IFLA) conference include: (1) "Medical Library Association: Organizational Change 1898 to Present--Illustrations from Continuing Education" (Raymond A. Palmer and M. Kent Mayfield, United States); (2)…

  7. Computers in Libraries Annual Conference (17th, Washington, DC, March 13-15, 2002): Collected Presentations.

    ERIC Educational Resources Information Center

    Nixon, Carol, Comp.

    This book contains presentations from the 17th annual Computers in Libraries Conference. Topics covered include: chatting with a librarian; verbots for library Web sites; collaborative IT (Information Technology) planning at Montgomery County Public Library (Maryland); designing a local government taxonomy; Weblogs; new roles for librarians in…

  8. The White House Conference on Library and Information Services (Washington, D.C., July 9-13, 1991).

    ERIC Educational Resources Information Center

    White House Conference on Library and Information Services.

    Designed for use by participants in the 1991 White House Conference on Library and Information Services (WHCLIS2), this guide begins with an overview of the White House Conferences on Library and Information Services, including background information on the 1979 Conference (WHCLIS) and an introduction to the 1991 Conference. Also in the first of…

  9. Libraries and Librarians: Making a Difference in the Knowledge Age. IFLA Council and General Conference: Conference Programme and Proceedings (67th, Boston, Massachusetts, August 16-25, 2001).

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This document contains the proceedings of the IFLA (International Federation of Library Associations and Institutions) 2001 conference, including the conference program and papers, and a list of participants. The theme of the conference was "Libraries and Librarians: Making a Difference in the Knowledge Age." Subthemes included: (1)…

  10. The Dilemma for Academic Librarians with Collection Development Responsibilities: A Comparison of the Value of Attending Library Conferences versus Academic Conferences

    ERIC Educational Resources Information Center

    Lyons, Lucy Eleonore

    2007-01-01

    Due to limitations imposed on professional travel, librarians with collection responsibilities must often choose between attending library conferences or relevant academic conferences. An illustrative comparison of the limitations and advantages of the American Library Association annual meeting and the annual conference of the American Political…

  11. The Off-Campus Library Services Conference Proceedings (7th, San Diego, California, October 25-27, 1995).

    ERIC Educational Resources Information Center

    Jacob, Carol J., Comp.

    This conference proceedings of off-campus library services includes 37 papers covering the following topics: ensuring excellence in distance library services; DePaul University's research consultation service; using videos to promote library service; a virtual academic library project in England; student-centered library instruction off campus;…

  12. Statistics in Support of the Ten Topic Areas. White House Conference on Library and Information Services.

    ERIC Educational Resources Information Center

    Keyser, Amy B., Ed.; LaMoure, Lawrence, Ed.

    This booklet presents statistical tables and graphs which were compiled to assist the delegates to the White House Conference on Library and Information Services in formulating their arguments. The tables and graphs have been assigned to one or more of the 10 topic areas, but may also be applied to the three conference themes of Literacy,…

  13. FLICC/FEDLINK Conference on Making Library Automation Choices (Washington, D.C., May 6, 1986).

    ERIC Educational Resources Information Center

    Landrum, Hollis

    This report of a conference convened by the Federal Library and Information Center Committee (FLICC) Subcommittee on Education provides brief summaries of four panel discussions conducted by 15 federal librarians who had assembled automation systems for their agencies' libraries and information centers. The first panel, consisting of Dean Hough,…

  14. Computers in Libraries Annual Conference (16th, Washington, DC, March 14-16, 2001): Collected Presentations.

    ERIC Educational Resources Information Center

    Nixon, Carol, Comp.

    This book contains the presentations of the 16th annual Computers in Libraries Conference. Contents include: "Creating New Services & Opportunities through Web Databases"; "Influencing Change and Student Learning through Staff Development"; "Top Ten Navigation Tips"; "Library of the Year: Gwinnett County…

  15. Sequential Art, the White House Conference, and School Library Media Specialists.

    ERIC Educational Resources Information Center

    Barron, Daniel D.

    2002-01-01

    Discusses sequential art, also known as comic books or graphic novels, as reading motivators, as content, as literature, and as the subject itself. Includes comic resources for school library media specialists and describes the White House Conference that Laura Bush has promoted that has given library media centers national visibility. (LRW)

  16. Proceedings of the Governor's Library Conference (1st, Albany, June 24-25, 1965).

    ERIC Educational Resources Information Center

    Governor's Library Conference, Albany, NY.

    An invitational conference, attended by 200 library executives, practitioners and persons from allied fields, was held to explore progress and problems in library development in New York State. Special consideration was given to: (1) the application of data processing techniques for reference and research and (2) the improvement of information…

  17. Computers in Libraries Annual Conference (16th, Washington, DC, March 14-16, 2001): Collected Presentations.

    ERIC Educational Resources Information Center

    Nixon, Carol, Comp.

    This book contains the presentations of the 16th annual Computers in Libraries Conference. Contents include: "Creating New Services & Opportunities through Web Databases"; "Influencing Change and Student Learning through Staff Development"; "Top Ten Navigation Tips"; "Library of the Year: Gwinnett County…

  18. "Fostering Library as Place for Distance Students: Best Practices from Two Universities"--Conference Report

    ERIC Educational Resources Information Center

    Cannady, Rachel E.

    2011-01-01

    In this article, the author reports on the Association of College and Research Libraries (ACRL) Conference which occurred in Philadelphia, Pennsylvania, March 29-April 2, 2011. There were numerous panel sessions to choose from, and the author attended "Fostering Library as Place for Distance Students: Best Practices from Two…

  19. FLICC/FEDLINK Conference on Making Library Automation Choices (Washington, D.C., May 6, 1986).

    ERIC Educational Resources Information Center

    Landrum, Hollis

    This report of a conference convened by the Federal Library and Information Center Committee (FLICC) Subcommittee on Education provides brief summaries of four panel discussions conducted by 15 federal librarians who had assembled automation systems for their agencies' libraries and information centers. The first panel, consisting of Dean Hough,…

  20. IFLA General Conference, 1985. Division on Management and Technology. Round Tables for the Management of Library Associations, Editors of Library Journals, and Audiovisual Media. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    This document includes papers presented at three round tables at the 1985 International Federation of Library Associations (IFLA) conference. Papers from the Round Table on Management of Library Associations include: (1) "Political Persuasion of Library Associations for the National Development of Libraries. The View of the Scandinavian…

  1. IFLA General Conference, 1989. Division of General Research Libraries. Section of National Libraries; Section of University Libraries and Other General Research Libraries; Section of Parliamentary Libraries. Booklet 10.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    There are 11 papers from the Division of General Research Libraries in this compilation: (1) "National Library and Information Needs: Alternative Ways of Meeting Them, with Special Reference to the Role of National Libraries" (Maurice B. Line); (2) "Managing Change in National LIbraries: The Library of Congress" (James H.…

  2. IFLA General Conference, 1992. Division of General Research Libraries: Section on National Libraries; Section on Parliamentary Libraries; Section on University Libraries and Other General Research Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    Fifteen papers delivered for the Division of General Research Libraries at the 1992 International Federation of Library Associations and Institutions annual meeting are presented. These papers deal with national libraries, parliamentary (legislative) libraries, and university libraries. The papers are: (1) "Seeking Alternatives to National…

  3. Biophotonics and immune responses-Highlights from a new SPIE photonics west conference (BIOS 2006).

    PubMed

    Chen, Wei R; Huang, Zheng

    2006-09-01

    This report summarizes some highlights from the "Biophotonics and Immune Responses", a new 2006 SPIE Photonics West Biomedical Optics (BIOS 2006) Conference. Some exciting recent progresses in host immune responses elicited by photodynamic therapy and other novel phototherapies are discussed.

  4. Immune TB Antibody Phage Display Library as a Tool To Study B Cell Immunity in TB Infections.

    PubMed

    Hamidon, Nurul Hamizah; Suraiya, Siti; Sarmiento, Maria E; Acosta, Armando; Norazmi, Mohd Nor; Lim, Theam Soon

    2017-09-07

    B cells and in particular antibodies has always played second fiddle to cellular immunity in regard to tuberculosis (TB). However, recent studies has helped position humoral immunity especially antibodies back into the foray in relation to TB immunity. Therefore, the ability to correlate the natural antibody responses of infected individuals toward TB antigens would help strengthen this concept. Phage display is an intriguing approach that can be utilized to study antibody-mediated responses against a particular infection via harvesting the B cell repertoire from infected individuals. The development of disease-specific antibody libraries or immune libraries is useful to better understand antibody-mediated immune responses against specific disease antigens. This study describes the generation of an immune single-chain variable fragment (scFv) library derived from TB-infected individuals. The immune library with an estimated diversity of 10(9) independent clones was then applied for the identification of monoclonal antibodies against Mycobacterium tuberculosis α-crystalline as a model antigen. Biopanning of the library isolated three monoclonal antibodies with unique gene usage. This strengthens the role of antibodies in TB immunity in addition to the role played by cellular immunity. The developed library can be applied against other TB antigens and aid antibody-derived TB immunity studies in the future.

  5. IFLA General Conference, 1985. Division on Education and Research. Section on Library Schools and Other Training Aspects. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The changing nature of library and information specialists' training and education is addressed in the following papers on library schools presented at the 1985 International Federation of Library Associations (IFLA) conference: (1) "Information Access and the Changing Library School Curriculum" (Herbert S. White, United States); (2)…

  6. IFLA General Conference, 1985. Division on Education and Research. Section on Library Schools and Other Training Aspects. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The changing nature of library and information specialists' training and education is addressed in the following papers on library schools presented at the 1985 International Federation of Library Associations (IFLA) conference: (1) "Information Access and the Changing Library School Curriculum" (Herbert S. White, United States); (2)…

  7. White House Conference on School Libraries: Keynote Address.

    ERIC Educational Resources Information Center

    Gregorian, Vartan

    2002-01-01

    Discusses the importance of libraries, books, and reading. Highlights include: the role of libraries in our culture; literacy, reading, and writing as the essence of thinking; the relationship between the book and the reader; and the impact of information technologies. (MES)

  8. Immune cell screening of a nanoparticle library improves atherosclerosis therapy

    PubMed Central

    Baxter, Samantha; Menon, Arjun; Alaarg, Amr; Sanchez-Gaytan, Brenda L.; Fay, Francois; Zhao, Yiming; Ouimet, Mireille; Braza, Mounia S.; Longo, Valerie A.; Abdel-Atti, Dalya; Duivenvoorden, Raphael; Calcagno, Claudia; Storm, Gert; Tsimikas, Sotirios; Moore, Kathryn J.; Swirski, Filip K.; Nahrendorf, Matthias; Fisher, Edward A.; Pérez-Medina, Carlos; Fayad, Zahi A.; Reiner, Thomas; Mulder, Willem J. M.

    2016-01-01

    Immunological complexity in atherosclerosis warrants targeted treatment of specific inflammatory cells that aggravate the disease. With the initiation of large phase III trials investigating immunomodulatory drugs for atherosclerosis, cardiovascular disease treatment enters a new era. We here propose a radically different approach: implementing and evaluating in vivo a combinatorial library of nanoparticles with distinct physiochemical properties and differential immune cell specificities. The library’s nanoparticles are based on endogenous high-density lipoprotein, which can preferentially deliver therapeutic compounds to pathological macrophages in atherosclerosis. Using the apolipoprotein E-deficient (Apoe−/−) mouse model of atherosclerosis, we quantitatively evaluated the library’s immune cell specificity by combining immunological techniques and in vivo positron emission tomography imaging. Based on this screen, we formulated a liver X receptor agonist (GW3965) and abolished its liver toxicity while still preserving its therapeutic function. Screening the immune cell specificity of nanoparticles can be used to develop tailored therapies for atherosclerosis and other inflammatory diseases. PMID:27791119

  9. IFLA General Conference, 1987. Division of Special Libraries. Biological and Medical Science Libraries Section. Social Science Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Six of the nine papers in this collection focus on biological and medical science libraries; the remaining three are concerned with social science libraries. The papers on biological and medical science libraries appear first in this list: (1) "Standards for Medical and Health Care Libraries: Canada" (Jan Greenwood, Canada); (2) "Standards for…

  10. IFLA General Conference, 1991. Division of General Research Libraries: Section of National Libraries; Section of University Libraries and Other General Research Libraries; Section of Parliamentary Libraries. Booklet 1.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    The 11 papers in this booklet were presented at meetings of 3 sections within the Division of General Research Libraries: (1) "On the Concept of Development of the National Library of the USSR" (N. I. Tyulina); (2) "Strategic Planning in National Libraries" (Maurice B. Line, UK); (3) "Strategic Planning in National…

  11. IFLA General Conference, 1987. Division of General Research Libraries. Parliamentary Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The seven papers in this collection describe parliamentary library services and activities in both developed and developing countries: (1) "The Electronic Catalogue of the Library of the Deutscher Bundestag" (Ernst Kohl, West Germany); (2) "Library of the Assembly of the Republic: Modernization Efforts" (A. Cabral, Portugal);…

  12. IFLA General Conference, 1987. Division of General Research Libraries. Parliamentary Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The seven papers in this collection describe parliamentary library services and activities in both developed and developing countries: (1) "The Electronic Catalogue of the Library of the Deutscher Bundestag" (Ernst Kohl, West Germany); (2) "Library of the Assembly of the Republic: Modernization Efforts" (A. Cabral, Portugal);…

  13. Off-Campus Library Services Conference Proceedings (8th, Providence, Rhode Island, April 22-24, 1998).

    ERIC Educational Resources Information Center

    Thomas, P. Steven, Comp.; Jones, Maryhelen, Comp.

    The 27 papers that make up this Proceedings were presented at the Eighth Off-Campus Library Services Conference. The primary objective of the conference was to provide a forum where practitioners involved in library services for off-campus constituents could gather to exchange relevant ideas, concerns and perspectives, and to share research.…

  14. Off-Campus Library Services Conference Proceedings (8th, Providence, Rhode Island, April 22-24, 1998).

    ERIC Educational Resources Information Center

    Thomas, P. Steven, Comp.; Jones, Maryhelen, Comp.

    The 27 papers that make up this Proceedings were presented at the Eighth Off-Campus Library Services Conference. The primary objective of the conference was to provide a forum where practitioners involved in library services for off-campus constituents could gather to exchange relevant ideas, concerns and perspectives, and to share research.…

  15. IFLA General Conference, 1987. Division of Special Libraries. Art Libraries Section. Round Table on Audiovisual Media. Administrative Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Seven of the 13 papers in this collection discuss art libraries; the remaining six papers are concerned with administrative and special libraries. The papers from the Section of Art Libraries appear first in this list: (1) "SCOAD (Standing Committee on Art Documentation): Its Inception and Aims" (Julian Gardner, United Kingdom); (2)…

  16. IFLA General Conference, 1987. Division of Libraries Serving the General Public. Children's Libraries Section. Public Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Four of the 17 papers in this collection focus on library services for children; the remaining 12 are concerned with public libraries. The papers on children's libraries appear first on this list: (1) "A Study of Integrated Services" (Selma K. Richardson, United States); (2) "Partnership and Prosper: A Broader View of Children's…

  17. Ohio's Literacy Situation. Ohio White House Conference on Library and Information Services for Literacy. Background Paper.

    ERIC Educational Resources Information Center

    Luther, Barbara

    Intended for use by participants in the Ohio Preconference and the 1991 White House Conference on Library and Information Services (WHCLIS), this background paper on one of the three major WHCLIS themes examines Ohio's literacy problem and reviews the state's current literacy programs and funding sources. Definitions of terms, a brief history of…

  18. Reports from the 2011 Missouri Library Association Annual Conference, October 5-7, 2011

    ERIC Educational Resources Information Center

    Miller, Andrea

    2012-01-01

    This article presents reports from the 2011 Missouri Library Association Annual Conference held on October 5-7, 2011. The first report, entitled "Online Information Literacy Instruction: Challenges and Strategies," was presented by Kristine Stewart and Kyle Denlinger, University of Missouri-Columbia. Stewart and Denlinger talk about…

  19. Reports from the 2011 Missouri Library Association Annual Conference, October 5-7, 2011

    ERIC Educational Resources Information Center

    Miller, Andrea

    2012-01-01

    This article presents reports from the 2011 Missouri Library Association Annual Conference held on October 5-7, 2011. The first report, entitled "Online Information Literacy Instruction: Challenges and Strategies," was presented by Kristine Stewart and Kyle Denlinger, University of Missouri-Columbia. Stewart and Denlinger talk about…

  20. Eight Key Issues for the White House Conference on Library and Information Services.

    ERIC Educational Resources Information Center

    American Society for Information Science, Washington, DC.

    Presented as a public service in anticipation of the October 1979 White House Conference on Library and Information Services, the eight papers in this booklet call attention to the key issues which the American Society for Information Science (ASIS) believes have developed from proliferating information and for technological advances. Each of the…

  1. White House Conference on Library and Information Services Follow-Up Inquiry. State/Territory Agency Responses.

    ERIC Educational Resources Information Center

    National Commission on Libraries and Information Science, Washington, DC.

    State and territory agency responses to a 1980 follow-up inquiry of the 1979 White House Conference on Library and Information Services (WHCLIST) are presented. Individual questionnaires were completed by 24 states and 2 trust territories. Questionnaires provide information on increased state aid to libraries, new library legislation,…

  2. White House Conference on Library and Information Services Follow-Up Inquiry. State/Territory Agency Responses.

    ERIC Educational Resources Information Center

    National Commission on Libraries and Information Science, Washington, DC.

    State and territory agency responses to a 1980 follow-up inquiry of the 1979 White House Conference on Library and Information Services (WHCLIST) are presented. Individual questionnaires were completed by 24 states and 2 trust territories. Questionnaires provide information on increased state aid to libraries, new library legislation,…

  3. IFLA General Conference, 1992. Presession Seminar on the Status, Reputation, and Image of the Library and Information Profession. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    Seven papers are presented from the presession of the 1992 International Federation of Library Associations and Institutions (IFLA) conference dealing with the status and reputation of the library and information professions, which continue to suffer a lack of image in society. Suggestions for improving the status of the library and information…

  4. Information for the 1980's. Final Report of the White House Conference on Library and Information Services, 1979.

    ERIC Educational Resources Information Center

    National Commission on Libraries and Information Science, Washington, DC.

    These proceedings of the 1979 White House Conference on Library and Information Services contain resolutions which are the basis for the proposed National Library and Information Services Program and for new national legislation. The resolutions reflect (1) the need to reshape library and information services in order to make them more responsive…

  5. Progress and Poverty; Library Association of Australia Proceedings of the Biennial Conference (16th, Sydney, August 1971).

    ERIC Educational Resources Information Center

    Whyte, Jean P., Ed.

    The complete program for this four-day conference is included in this proceedings. The papers are grouped into the following major sections: Official Opening, Greetings from American Library Association, Presidential Address, Plenary Papers, General Papers, Archives Section Papers, Public Libraries Section Papers, Children's Libraries Section…

  6. The Off-Campus Library Services Conference Proceedings (6th, Kansas City, Missouri, October 6-8, 1993).

    ERIC Educational Resources Information Center

    Jacob, Carol J., Comp.

    This conference proceedings of off-campus library services includes 30 papers covering the following topics: extracting the hidden potential of course syllabi; obstacles to user education for off-campus students; using Hypercard in an off-campus library environment; satisfying accreditation requirements in lean times; the library's role in…

  7. IFLA General Conference, 1987. Division of Special Libraries. Science and Technology Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The six papers in this collection focus on science and technology libraries: (1) "Human Aspects of Electronically-Stored Information: The Library User" (A. J. Meadows, United Kingdom); (2) "Untersuchung Menschlicher Aspekte bei den Elektronisch Gespeicherten Informationen: Ansichten des Leiters eines Bibliothekskollektives = Human Aspects of…

  8. Libraries: Vital to Tomorrow's World; British Columbia Centennial Citizens' Conference on Libraries (Victoria, May 3, 1971).

    ERIC Educational Resources Information Center

    British Columbia Library Development Commission, Vancouver. Committee on Library Development.

    The purpose of this meeting of an invited group of concerned citizens was to focus attention on the interrelation of all types of libraries in British Columbia. It was intended to involve those who pay for library service in a discussion of its potential to meet the demands of the coming generation. This record, which is not intended to be a…

  9. IFLA General Conference, 1987. Division of Libraries Serving the General Public. School Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Four papers are compiled in the section on school libraries: (1) "Zentrale Dienstleistungen fur Schulbibliotheken in der Bundesrepublik Deutschland am Beispiel der Schulbibliothekarischen Arbeitsstelle der Stadtbucherei Frankfurt am Main = Centralized School Library Services in the Federal Republic of Germany, as Performed at the School Library…

  10. Improved vectors for expression library immunization--application to Mycoplasma hyopneumoniae infection in pigs.

    PubMed

    Moore, R J; Lenghaus, C; Sheedy, S A; Doran, T J

    2001-10-12

    Expression library immunization (ELI) has previously been used in a number of disease models in mice. Here, we describe the first example of the application of ELI to a large animal model with the immunization of pigs against enzootic pneumonia, a disease caused by Mycoplasma hyopneumoniae. The development of new plasmid vectors and library screening methods facilitated the application of ELI to this disease by allowing random libraries to be screened for clones expressing recombinant proteins. In this way the vast majority of clones in random libraries that are unproductive can be eliminated, meaning that libraries are more likely to give protection and are subsequently easier to further screen and analyze. By using this approach we have used one library screen and two animal trials to progress from an original library of 20,000 clones to a group of just 96 clones.

  11. Libraries--Link To Learning. Final Report on the Indiana Governor's Conference on Libraries and Information Services (2nd, Indianapolis, Indiana, November 16-18, 1990).

    ERIC Educational Resources Information Center

    Henson, Jane E., Ed.

    This report focuses on the three themes of the 1991 White House Conference on Library and Information Services, i.e., Democracy, Literacy, and Productivity. The report begins with an introduction by State Librarian Ray Ewick and a summary of the conference activities. Executive summaries of remarks made by speakers during three general conference…

  12. Libraries for Life: Democracy, Diversity, Delivery. IFLA Council and General Conference: Conference Programme and Proceedings (68th, Glasgow, Scotland, August 18-24, 2002).

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This document presents the program and proceedings from the 68th International Federation of Library Associations and Institutions (IFLA) Conference held in Glasgow, Scotland, August 18-24, 2002. Topics of presentations include: library services for parliaments; needs assessment; the effects of September 11th on information provision and privacy;…

  13. Libraries Without Walls 4: The Delivery of Library Services to Distant Users. Proceedings of an International Conference Organized by the Centre for Research in Library and Information Management (CERLIM), Manchester Metropolitan University (Manchester, England, September 14-18, 2001).

    ERIC Educational Resources Information Center

    Brophy, Peter, Ed.; Fisher, Shelagh, Ed.; Clarke, Zoe, Ed.

    This proceedings of the fourth Libraries Without Walls Conference addresses key strategic issues arising from international, regional, and cross-sectoral approaches to the provision of library services to distant users. The following papers are included: Theme 1: Libraries and Virtual Learning Environments: "Introduction" (Peter Brophy,…

  14. IFLA General Conference, 1985. Division on Libraries Serving the General Public. Round Table on Library Service to Ethnic and Linguistic Minorities. Papers. the General Public. Round Table on Library Service to Ethnic and Linguistic Minorities. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library services to ethnic and linguistic minorities which were presented at the 1985 International Federation of Library Associations (IFLA) conference include: (1) "On Serving the Older Polyglot: The Role of the Public Libraries in the United States" (Sylva N. Manoogian, United States); (2) "Minority Language Selection and Acquisition…

  15. Libraries Without Walls 4: The Delivery of Library Services to Distant Users. Proceedings of an International Conference Organized by the Centre for Research in Library and Information Management (CERLIM), Manchester Metropolitan University (Manchester, England, September 14-18, 2001).

    ERIC Educational Resources Information Center

    Brophy, Peter, Ed.; Fisher, Shelagh, Ed.; Clarke, Zoe, Ed.

    This proceedings of the fourth Libraries Without Walls Conference addresses key strategic issues arising from international, regional, and cross-sectoral approaches to the provision of library services to distant users. The following papers are included: Theme 1: Libraries and Virtual Learning Environments: "Introduction" (Peter Brophy,…

  16. IFLA General Conference, 1984. Libraries Serving the General Public Division. Section on Children's Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on children's services presented at the 1984 IFLA general conference include: (1) "Children's Literature in Kenya: Staggering Problems, Limited Successes, and Some Signs of Hope" (Ellen Kitonga, Kenya), which identifies some basic problems with respect to children's literature in Africa and suggests possible courses of action; (2)…

  17. IFLA General Conference, 1987. Division of Special Libraries. Geography and Map Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The four papers in this collection report on geography and map libraries: (1) "Changing Patterns of Map Availability" (C. R. Perkins and R. B. Parry, United Kingdom), which presents the results of a three year survey into the state of world mapping, a discussion of the implications of changing patterns of map availability for map library…

  18. Brick & Click Libraries: An Academic Library Conference (14th, Maryville, Missouri, November 7, 2014)

    ERIC Educational Resources Information Center

    Baudino, Frank, Ed.; Johnson, Carolyn, Ed.

    2014-01-01

    Eighteen scholarly papers and twelve abstracts comprise the content of the fourteenth annual Brick and Click Libraries Symposium, held annually at Northwest Missouri State University in Maryville, Missouri. The proceedings, authored by academic librarians and presented at the symposium, portray the contemporary and future face of librarianship.…

  19. IFLA General Conference, 1987. Division of Special Libraries. Geography and Map Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The four papers in this collection report on geography and map libraries: (1) "Changing Patterns of Map Availability" (C. R. Perkins and R. B. Parry, United Kingdom), which presents the results of a three year survey into the state of world mapping, a discussion of the implications of changing patterns of map availability for map library…

  20. Construction and Screening of Antigen Targeted Immune Yeast Surface Display Antibody Libraries

    SciTech Connect

    Miller, Keith D.; Pefaur, Noah B.; Baird, Cheryl L.

    2008-07-01

    These protocols describe a yeast surface display-based process for the rapid selection of antibodies from immunized mice, eliminating the need for creating and screening hybridoma fusions. A yeast surface display library of single-chain antibody fragments (scFvs) is created from antigen-binding B cells from the splenocytes of immunized mice. The antigen targeted library is then screened for antigen specific scFv by magnetic activated cell sorting (MACS) and fluorescence activated cell sorting (FACS). Library construction and screening can be accomplished in as little as 2 weeks resulting in a panel of scFvs specific for the target antigen.

  1. Construction and Screening of Antigen Targeted Immune Yeast Surface Display Antibody Libraries

    SciTech Connect

    Miller, Keith D.; Pefaur, Noah B.; Baird, Cheryl L.

    2009-08-02

    These protocols describe a yeast surface display-based process for the rapid selection of antibodies from immunized mice, eliminating the need for creating and screening hybridoma fusions. A yeast surface display library of single-chain antibody fragments (scFvs) is created from antigen-binding B cells from the splenocytes of immunized mice. The antigen targeted library is then screened for antigen specific scFv by magneticactivated cell sorting (MACS) and fluorescence-activated cell sorting (FACS). Library construction and screening can be accomplished in as little as 2 weeks, resulting in a panel of scFvs specific for the target antigen.

  2. Economic Vitality: Challenge and Opportunity. Selected Readings from the Fall Conference of the Kansas Library Association: Public Library Section (Hutchinson, Kansas, September 28-29, 1988). Monograph Series Number 9.

    ERIC Educational Resources Information Center

    Emporia State Univ., KS. School of Library and Information Management.

    In September 1988, the Public Library/Trustees Section of the Kansas Library Association, with the support of the School of Library and Information Management at Emporia State University and the Kansas State Library, held a conference on economic vitality. Following a "Review and Preview" by Martha Hale, Dean of the School of Library and…

  3. Southwestern Library Association Conference (23rd, Fort Worth, Texas, November 5-7, 1970). Papers and Proceedings.

    ERIC Educational Resources Information Center

    Brawner, Lee B., Ed.

    Part 1 of this conference consists of the six papers presented: (1) "Readers and Reading in 1980," (2) "A Futuristic View of Libraries, Information Transfer, and Media Forms," (3) "A Relative Influence of the Bookseller and the Library on the Publishing Decision," (4) "World Without Walls," (5) "Getting…

  4. Conference on Inter-Library Cooperation and Exchange, San Juan, Puerto Rico, April 30 - May 2, 1969.

    ERIC Educational Resources Information Center

    Virgin Islands Dept. of Conservation and Cultural Affairs, St. Thomas. Div. of Libraries and Museums.

    The Conference had as its main objective the assembling of library administrators and librarians to consider all aspects relating to the implementation of a library cooperative plan for the interchange of publications, photoduplication services and bibliographic information. Papers that were presented are: (1) "Automation and Bibliographic…

  5. Identifying Liaison Opportunities through Content Analysis: Academic Library Trends in the Ecological Society of America's Conference Program

    ERIC Educational Resources Information Center

    Conklin, Jamie L.

    2013-01-01

    Science and technology librarians need to continually invest time into professional development activities to gain new skills relevant to the faculty and students they serve. Many academic libraries face diminishing budgets and have few travel dollars available for attendance at library and subject-specific conferences. This study determined that…

  6. Identifying Liaison Opportunities through Content Analysis: Academic Library Trends in the Ecological Society of America's Conference Program

    ERIC Educational Resources Information Center

    Conklin, Jamie L.

    2013-01-01

    Science and technology librarians need to continually invest time into professional development activities to gain new skills relevant to the faculty and students they serve. Many academic libraries face diminishing budgets and have few travel dollars available for attendance at library and subject-specific conferences. This study determined that…

  7. Autopolyreactivity Confers a Holistic Role in the Immune System.

    PubMed

    Avrameas, S

    2016-04-01

    In this review, we summarize and discuss some key findings from the study of naturally occurring autoantibodies. The B-cell compartment of the immune system appears to recognize almost all endogenous and environmental antigens. This ability is accomplished principally through autopolyreactive humoral and cellular immune receptors. This extended autopolyreactivity (1) along immunoglobulin gene recombination contributes to the immune system's ability to recognize a very large number of self and non-self constituents; and (2) generates a vast immune network that creates communication channels between the organism's interior and exterior. Thus, the immune system continuously evolves depending on the internal and external stimuli it encounters. Furthermore, this far-reaching network's existence implies activities resembling those of classical biological factors or activities that modulate the function of other classical biological factors. A few such antibodies have already been found. Another important concept is that natural autoantibodies are highly dependent on the presence or absence of commensal microbes in the organism. These results are in line with past and recent findings showing the fundamental influence of the microbiota on proper immune system development, and necessitate the existence of a host-microbe homeostasis. This homeostasis requires that the participating humoral and cellular receptors are able to recognize self-antigens and commensal microbes without damaging them. Autopolyreactive immune receptors expressing low affinity for both types of antigens fulfil this role. The immune system appears to play a holistic role similar to that of the nervous system. © 2016 The Foundation for the Scandinavian Journal of Immunology.

  8. IFLA General Conference, 1989. Division of Libraries Serving the General Public. Section on Children's Libraries; Section of School Libraries; Section of Libraries Serving Disadvantaged Persons. Booklet 30.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Fifteen papers from the Division of Libraries Serving the General Public are included in this collection: "When the Children's Library Meets the Museum" (French and English versions; Annie Pissard); "Value of Library Service for Children Literature in France" (Aline Eisenegger); "The Latin American Literature in France" (Aline Eisenegger); "The…

  9. IFLA General Conference, 1992. Division of Libraries Serving the General Public: Section on Library Services to Multicultural Populations; Section on School Libraries; Section on Public Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    Eleven papers delivered for the Division of Libraries Serving the General Public at the International Federation of Library Associations and Institutions 1992 annual meeting are presented. Most deal with library services to multicultural populations, including those of developing countries. The following papers are included: (1) "Library…

  10. Strategies to potentiate immune response after photodynamic therapy (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Hamblin, Michael R.

    2017-02-01

    Photodynamic therapy (PDT) has been used as a cancer therapy for forty years but has not yet advanced to a mainstream cancer treatment. Although PDT has been shown to be an efficient photochemical way to destroy local tumors by a combination of non-toxic dyes and harmless visible light, it is its additional effects in mediating the stimulation of the host immune system that gives PDT a great potential to become more widely used. Although the stimulation of tumor-specific cytotoxic T-cells that can destroy distant tumor deposits after PDT has been reported in some animal models, it remains the exception rather than the rule. This realization has prompted several investigators to test various combination approaches that could potentiate the immune recognition of tumor antigens that have been released after PDT. Some of these combination approaches use immunostimulants including various microbial preparations that activate Toll-like receptors and other receptors for pathogen associated molecular patterns. Other approaches use cytokines and growth factors whether directly administered or genetically encoded. A promising approach targets regulatory T-cells. We believe that by understanding the methods employed by tumors to evade immune response and neutralizing them, more precise ways of potentiating PDT-induced immunity can be devised.

  11. Laura Bush: Papers Presented at the White House on the Occasion of the White House Conference on School Libraries, June 4, 2002.

    ERIC Educational Resources Information Center

    Bush, Laura; Martin, Robert; Gregorian, Vartan; Neuman, Susan; Lance, Keith Curry; Kimsey-Pharr, Faye; Hartzell, Gary; Smith, Kathleen D.; Wisely, Steve; DeVita, M. Christine

    2002-01-01

    Includes nine papers that were presented at the White House Conference on School Libraries in 2002. Highlights include the importance of books and access to information in today's society; the role of school librarians; the importance of school libraries; how to change perceptions of school libraries; and how school libraries can improve student…

  12. Laura Bush: Papers Presented at the White House on the Occasion of the White House Conference on School Libraries, June 4, 2002.

    ERIC Educational Resources Information Center

    Bush, Laura; Martin, Robert; Gregorian, Vartan; Neuman, Susan; Lance, Keith Curry; Kimsey-Pharr, Faye; Hartzell, Gary; Smith, Kathleen D.; Wisely, Steve; DeVita, M. Christine

    2002-01-01

    Includes nine papers that were presented at the White House Conference on School Libraries in 2002. Highlights include the importance of books and access to information in today's society; the role of school librarians; the importance of school libraries; how to change perceptions of school libraries; and how school libraries can improve student…

  13. The evolution of secondary organization in immune system gene libraries

    SciTech Connect

    Hightower, R.; Forrest, S.; Perelson, A.S.

    1993-02-01

    A binary model of the immune system is used to study the effects of evolution on the genetic encoding for antibody molecules. We report experiments which show that the evolution of immune system genes, simulated by the genetic algorithm, can induce a high degree of genetic organization even though that organization is not explicitly required by the fitness function. This secondary organization is related to the true fitness of an individual, in contrast to the sampled fitness which is the explicit fitness measure used to drive the process of evolution.

  14. Nashville '90: Annual Meeting of the White House Conference on Library and Information Services Taskforce (WHCLIST) (11th, Nashville, Tennessee, August 16-18, 1990). Final Conference Report.

    ERIC Educational Resources Information Center

    Gleaves, Edwin S., Ed.

    This report provides outlines of the reports, comments, and recommendations which emanated from the 3-day Tennessee Library and Information Services Taskforce conference. Representing the first and second general sessions, and included in this report, are the opening remarks; reports from Washington, D.C. on the status of plans for the second…

  15. IFLA General Conference, 1986. Pre-Conference Seminar on Automated Systems for Access to Multilingual and Multiscript Library materials: Problems and Solutions. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    A seminar which considered problems and solutions regarding automated systems for access to multilingual and multiscript library materials was held as a pre-session before the IFLA general conference in 1986. Papers presented include: (1) "Romanized and Transliterated Databases of Asian Language Materials--History, Problems, and…

  16. Academic Libraries: Myths and Realities. Proceedings of the National Conference of the Association of College and Research Libraries (3rd, Seattle, Washington, April 4-7, 1984).

    ERIC Educational Resources Information Center

    Dodson, Suzanne C., Ed.; Menges, Gary L., Ed.

    The 56 papers in this proceedings are divided into three groups--theme, alternative format, and contributed papers. Myths and realities are explored in the first group as they relate to university, college, community, and junior college libraries, and to private sector information utilities. The conference wrap-up session is included in this…

  17. Academic Libraries: Myths and Realities. Proceedings of the National Conference of the Association of College and Research Libraries (3rd, Seattle, Washington, April 4-7, 1984).

    ERIC Educational Resources Information Center

    Dodson, Suzanne C., Ed.; Menges, Gary L., Ed.

    The 56 papers in this proceedings are divided into three groups--theme, alternative format, and contributed papers. Myths and realities are explored in the first group as they relate to university, college, community, and junior college libraries, and to private sector information utilities. The conference wrap-up session is included in this…

  18. Fundamental study of expression-library immunization against Taenia taeniaeformis in rats.

    PubMed

    Okamoto, Munehiro; Matsumoto, Kanako; Shibui, Akiko; Shibahara, Toshiyuki; Ito, Akira

    2005-01-01

    Expression-library immunization has been proposed as an effective means to screen a large number of genes of the pathogen as candidate protective molecules. In this study, we examined the efficacy of expression-library immunization using a T. taeniaeformis rat model system. Total RNAs were isolated from the last 15 segments of adult T. taeniaeformis and poly A RNA was purified. cDNA library was produced using SuperScript Plasmid System, which contains a mammalian expression vector, pCMV*SPORT6. From about 3,500 clones examined, more than 800 clones were found to contain DNA fragments. About 200 clones were sequenced and the homology search was carried out. The blast search revealed that 29% of the expression genes were mitochondrial genes (rRNA; 17%, protein; 12%). Nuclear rRNA genes (10%), nuclear protein (9%) and genes from Escherichia coli were also detected. Forty-two percent of sequences did not show a significant similarity to any genes deposited in the public database. Rats were immunized with expression-library and injected orally with 1,000 T. taeniaeformis eggs. However the protective effect of expression-library vaccine was not confirmed.

  19. Identification of Immune-Relevant Factors Conferring Sarcoidosis Genetic Risk

    PubMed Central

    Fischer, Annegret; Ellinghaus, David; Nutsua, Marcel; Hofmann, Sylvia; Montgomery, Courtney G.; Iannuzzi, Michael C.; Rybicki, Benjamin A.; Petrek, Martin; Mrazek, Frantisek; Pabst, Stefan; Grohé, Christian; Grunewald, Johan; Ronninger, Marcus; Eklund, Anders; Padyukov, Leonid; Mihailovic-Vucinic, Violeta; Jovanovic, Dragana; Sterclova, Martina; Homolka, Jiri; Nöthen, Markus M.; Herms, Stefan; Gieger, Christian; Strauch, Konstantin; Winkelmann, Juliane; Boehm, Bernhard O.; Brand, Stephan; Büning, Carsten; Schürmann, Manfred; Ellinghaus, Eva; Baurecht, Hansjörg; Lieb, Wolfgang; Nebel, Almut; Müller-Quernheim, Joachim; Franke, Andre

    2015-01-01

    Rationale: Genetic variation plays a significant role in the etiology of sarcoidosis. However, only a small fraction of its heritability has been explained so far. Objectives: To define further genetic risk loci for sarcoidosis, we used the Immunochip for a candidate gene association study of immune-associated loci. Methods: Altogether the study population comprised over 19,000 individuals. In a two-stage design, 1,726 German sarcoidosis cases and 5,482 control subjects were genotyped for 128,705 single-nucleotide polymorphisms using the Illumina Immunochip for the screening step. The remaining 3,955 cases, 7,514 control subjects, and 684 parents of affected offspring were used for validation and replication of 44 candidate and two established risk single-nucleotide polymorphisms. Measurements and Main Results: Four novel susceptibility loci were identified with genome-wide significance in the European case-control populations, located on chromosomes 12q24.12 (rs653178; ATXN2/SH2B3), 5q33.3 (rs4921492; IL12B), 4q24 (rs223498; MANBA/NFKB1), and 2q33.2 (rs6748088; FAM117B). We further defined three independent association signals in the HLA region with genome-wide significance, peaking in the BTNL2 promoter region (rs5007259), at HLA-B (rs4143332/HLA-B*0801) and at HLA-DPB1 (rs9277542), and found another novel independent signal near IL23R (rs12069782) on chromosome 1p31.3. Conclusions: Functional predictions and protein network analyses suggest a prominent role of the drug-targetable IL23/Th17 signaling pathway in the genetic etiology of sarcoidosis. Our findings reveal a substantial genetic overlap of sarcoidosis with diverse immune-mediated inflammatory disorders, which could be of relevance for the clinical application of modern therapeutics PMID:26051272

  20. Proceedings of the National Conference on Population Library and Information Services (3rd Chapel Hill, N. C., May 14-15, 1970)

    ERIC Educational Resources Information Center

    Kellermann, Priscilla

    Contains the proceedings of the Third National Conference on Population Library and Information Services held in Chapel Hill, North Carolina, May 14-15, 1970. Under the joint sponsorship of the Carolina Population Center and the Population Council of New York, this was the third in a series of annual conference/workshops devoted to library and…

  1. IFLA General Conference, 1990. Division of Special Libraries: Section of Administrative Libraries; Section of Social Science Libraries; Section of Science and Technology Libraries; Section of Biological and Medical Sciences Libraries; Section of Art Libraries. Booklet 2.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 23 papers in this collection were presented at 5 sections of the Division of Special Libraries: (1) "Principles of Government Librarianship" (Hans H. van der Neut); (2) "Strategic Planning as an Instrument of Improving Library Quality" (Maurice B. Line); (3) "Library Staff Development Consultancy: A Means to Achieve a…

  2. IFLA General Conference 1988. Division of General Research Libraries. Section on National Libraries; Section on Parliamentary Libraries; Section on University Libraries and Other General Research Libraries; Section on Library History.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 12 papers in this compilation focus on the activities of general research libraries, i.e., national, parliamentary, and university libraries: (1) "Commercial and Revenue Raising Activities in National Libraries" (Maurice Line & Peter Scott, New Zealand); (2) "The End of All and Forever--On the Acquisition Policies of…

  3. IFLA General Conference, 1990. Division of Special Libraries: Section of Administrative Libraries; Section of Social Science Libraries; Section of Science and Technology Libraries; Section of Biological and Medical Sciences Libraries; Section of Art Libraries. Booklet 2.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 23 papers in this collection were presented at 5 sections of the Division of Special Libraries: (1) "Principles of Government Librarianship" (Hans H. van der Neut); (2) "Strategic Planning as an Instrument of Improving Library Quality" (Maurice B. Line); (3) "Library Staff Development Consultancy: A Means to Achieve a…

  4. Identification by genomic immunization of a pool of DNA vaccine candidates that confer protective immunity in mice against Neisseria meningitidis serogroup B.

    PubMed

    Yero, Daniel; Pajón, Rolando; Pérez, Yusleydis; Fariñas, Mildrey; Cobas, Karem; Diaz, Daiyana; Solis, Rosa L; Acosta, Armando; Brookes, Charlotte; Taylor, Stephen; Gorringe, Andrew

    2007-07-09

    We have shown previously that expression library immunization is viable alternative approach to induce protective immunity against Neisseria meningitidis serogroup B. In this study we report that few rounds of library screening allow identification of protective pools of defined antigens. A previously reported protective meningococcal library (L8, with 600 clones) was screened and two sub-libraries of 95 clones each were selected based on the induction of bactericidal and protective antibodies in BALB/c mice. After sequence analysis of each clone within these sub-libraries, we identified a pool of 20 individual antigens that induced protective immune responses in mice against N. meningitidis infection, and the observed protection was associated with the induction of bactericidal antibodies. Our studies demonstrate for the first time that ELI combined with sequence analysis is a powerful and efficient tool for identification of candidate antigens for use in a meningococcal vaccine.

  5. CD31 signals confer immune privilege to the vascular endothelium

    PubMed Central

    Cheung, Kenneth; Ma, Liang; Wang, Guosu; Coe, David; Ferro, Riccardo; Falasca, Marco; Buckley, Christopher D.; Mauro, Claudio; Marelli-Berg, Federica M.

    2015-01-01

    Constitutive resistance to cell death induced by inflammatory stimuli activating the extrinsic pathway of apoptosis is a key feature of vascular endothelial cells (ECs). Although this property is central to the maintenance of the endothelial barrier during inflammation, the molecular mechanisms of EC protection from cell-extrinsic, proapoptotic stimuli have not been investigated. We show that the Ig-family member CD31, which is expressed by endothelial but not epithelial cells, is necessary to prevent EC death induced by TNF-α and cytotoxic T lymphocytes in vitro. Combined quantitative RT-PCR array and biochemical analysis show that, upon the engagement of the TNF receptor with TNF-α on ECs, CD31 becomes activated and, in turn, counteracts the proapoptotic transcriptional program induced by TNF-α via activation of the Erk/Akt pathway. Specifically, Akt activation by CD31 signals prevents the localization of the forkhead transcription factor FoxO3 to the nucleus, thus inhibiting transcription of the proapoptotic genes CD95/Fas and caspase 7 and de-repressing the expression of the antiapoptotic gene cFlar. Both CD31 intracellular immunoreceptor tyrosine-based inhibition motifs are required for its prosurvival function. In vivo, CD31 gene transfer is sufficient to recapitulate the cytoprotective mechanisms in CD31− pancreatic β cells, which become resistant to immune-mediated rejection when grafted in fully allogeneic recipients. PMID:26392551

  6. Total Quality Management in Academic Libraries: Initial Implementation Efforts. Proceedings from the International Conference on TQM and Academic Libraries (1st, Washington, D.C., April 20-22, 1994).

    ERIC Educational Resources Information Center

    Rounds, Laura, Ed.; Matthews, Michael, Ed.

    This document contains conference papers, other speeches, and supplementary material from the first International Conference on Total Quality Management (TQM) and Academic Libraries, held in 1994. The conference was comprised of four sessions, and the introductory remarks of each are included, along with transcriptions of each session's…

  7. IFLA General Conference, 1984. Education and Research Division. Section on Library Schools and Other Training Aspects. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on the education and training of librarians presented at the 1984 IFLA general conference include: (1) "Training and Developing a Professional for the Widening Library Responsibilities: A Management View," in which Charles K. Wambugu (Kenya) examines the management requirements for a professional librarian and discusses the role…

  8. Information Management. VALA National Conference on Library Automation Proceedings (2nd, Melbourne, Australia, November 28-December 1, 1983). Volume 2.

    ERIC Educational Resources Information Center

    Cheney, B. J., Ed.

    The second of two volumes of a conference preceedings, this volume contains the preprints of five submitted papers, which are published as submitted by the authors: M. Broadbent and L. B. McIntyre, "Information Management: Promise and Reality"; E. Spoor, "Management Information from Library Systems"; R. Walsh, "Integrated…

  9. Proceedings of the Conference on Application of Scanning Methodologies in Libraries (Beltsville, Maryland, November 17-18, 1988).

    ERIC Educational Resources Information Center

    Blamberg, Donald L., Ed.; And Others

    Planned and organized by the National Agricultural Library (NAL), the conference was designed to bring together people involved in scanning projects and to evaluate the feasibility of using hand-held scanning devices for transcribing bibliographic information during the cataloging and indexing processes. Ten previously unpublished papers and a…

  10. IFLA General Conference, 1986. Pre-Session Seminar on Special Libraries and Their Role in National Development. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    A seminar on special libraries and their role in national development was held as a pre-session before the IFLA conference in 1986. Papers presented include: (1) "On the Problem of Document Delivery in the Field of Science and Technology in Asia" (Daxun He, People's Republic of China); (2) "The Planning and Development of Special…

  11. Education in Library and Information Science. Proceedings of the International Conference (Dubrovnik, Yugoslavia, May 21-26, 1984).

    ERIC Educational Resources Information Center

    Mihel, Ivan, Ed.; Tudor-Silovic, Neva, Ed.

    1984-01-01

    An international conference attended by 59 participants from 12 countries was organized to present Yugoslavia as a case study to the international audience, to bring to the Yugoslav audience a variety of international experiences in library and information science education and training, and to acquaint participants with some of the new…

  12. IFLA General Conference, 1986. Pre-Session Seminar on Special Libraries and Their Role in National Development. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    A seminar on special libraries and their role in national development was held as a pre-session before the IFLA conference in 1986. Papers presented include: (1) "On the Problem of Document Delivery in the Field of Science and Technology in Asia" (Daxun He, People's Republic of China); (2) "The Planning and Development of Special…

  13. Information Management. VALA National Conference on Library Automation Proceedings (2nd, Melbourne, Australia, November 28-December 1, 1983). Volume 2.

    ERIC Educational Resources Information Center

    Cheney, B. J., Ed.

    The second of two volumes of a conference preceedings, this volume contains the preprints of five submitted papers, which are published as submitted by the authors: M. Broadbent and L. B. McIntyre, "Information Management: Promise and Reality"; E. Spoor, "Management Information from Library Systems"; R. Walsh, "Integrated…

  14. IFLA General Conference 1988. Open Forum on IFLA Core Programs; Section on INTAMEL; Libraries Serving Disadvantaged Persons; Libraries for the Blind; Children's Libraries; School Libraries.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Twenty-one papers from the Open Forum on Core Programs are included in this collection: (1) "The IFLA (International Federal of Library Associations) UAP (Universal Availability of Publications) Programme" (M. F. Plassard, IFLA Programme Officer); (2) "Progress Report on IFLA Core Programme for Universal Dataflow and…

  15. IFLA General Conference, 1987. Division of General Research Libraries. National Libraries Section. University Libraries and Other General Research Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Of the 11 papers in this collection, three are in German and in English translation; one is in French with an English translation and two are in French only; one is in Spanish with an English translation; and four are in English. The papers include: (1) "Europaische Bibliotheksinitiativen = European Library Initiatives" (Gunther Pflug); (2) "Die…

  16. IFLA General Conference, 1987. Division of General Research Libraries. National Libraries Section. University Libraries and Other General Research Libraries Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Of the 11 papers in this collection, three are in German and in English translation; one is in French with an English translation and two are in French only; one is in Spanish with an English translation; and four are in English. The papers include: (1) "Europaische Bibliotheksinitiativen = European Library Initiatives" (Gunther Pflug); (2) "Die…

  17. Oral immunization with recombinant Lactococcus lactis confers protection against respiratory pneumococcal infection.

    PubMed

    Villena, Julio; Medina, Marcela; Raya, Raúl; Alvarez, Susana

    2008-10-01

    In the present work, we evaluated if oral immunization with the pneumococcal protective protein A (PppA), expressed in the cell wall of Lactococcus lactis (L. lactis PppA+), was able to confer protective immunity against Streptococcus pneumoniae. Mice were immunized orally with L. lactis PppA+ for 5 consecutive days. Vaccination was performed one (nonboosted group) or 2 times with a 2 week interval between each immunization (boosted group). Oral priming with L. lactis PppA+ induced the production of anti-PppA IgM, IgG, and IgA antibodies in serum and in bronchoalveolar (BAL) and intestinal (IF) lavage fluids. Boosting with L. lactis PppA+ increased the levels of mucosal and systemic immunoglobulins. Moreover, the avidity and the opsonophagocytic activity of anti-PppA antibodies were significantly improved in the boosted group. The presence of both IgG1 and IgG2a anti-PppA antibodies in serum and BAL and the production of both interferon gamma and interleukin-4 by spleen cells from immunized mice indicated that L. lactis PppA+ stimulated a mixture of Th1 and Th2 responses. The ability of L. lactis PppA+ to confer cross-protective immunity was evaluated using challenge assays with serotypes 3, 6B, 14, and 23F. Lung bacterial cell counts and hemocultures showed that immunization with L. lactis PppA+ improved resistance against all the serotypes assessed, including serotype 3, which was highly virulent in our experimental animal model. To our knowledge, this is the first demonstration of protection against respiratory pneumococcal infection induced by oral administration of a recombinant lactococcal vaccine.

  18. A Colorado Response to the Information Society: The Changing Academic Library. Proceedings of a Conference (Denver, Colorado, October 6-7, 1983).

    ERIC Educational Resources Information Center

    Breivik, Patricia Senn, Ed.

    As suggested by a Colorado Academic Library Master Plan developed in 1982, a statewide conference that brought together academicians and librarians was held to explore the role of academic libraries in the information society. People came in teams of three from institutions and included library directors, academic vice presidents, and faculty…

  19. Duration of protective immunity conferred by maternal tetanus toxoid immunization: further evidence from Matlab, Bangladesh.

    PubMed Central

    Koenig, M A; Roy, N C; McElrath, T; Shahidullah, M; Wojtyniak, B

    1998-01-01

    OBJECTIVES: Although maternal tetanus immunization has been shown to be highly effective in the prevention of neonatal tetanus, unresolved questions remain concerning the required minimum number of doses and the resulting duration of effective immunity. This study examined the duration of effective immunity against neonatal tetanus provided by maternal tetanus immunization. METHODS: A randomized, double-blind cholera vaccine trial of 41,571 children and nonpregnant adult women carried out in 1974 in the Matlab comparison area of rural Bangladesh provided a unique opportunity to address dose and immunity issues. RESULTS: Children of women who received either 1 or 2 injections of tetanus toxoid experienced 4- to 14-day mortality levels consistently lower than those of children of unimmunized mothers. Analysis of neonatal-tetanus-related mortality showed that 2 injections of tetanus toxoid provided significant protection for subsequent durations of up to 12 or 13 years. CONCLUSIONS: The data demonstrate that a limited-dose regimen of maternal tetanus toxoid provides significant and extended protection against the risk of neonatal tetanus death. PMID:9618617

  20. Discovering naturally processed antigenic determinants that confer protective T cell immunity

    PubMed Central

    Gilchuk, Pavlo; Spencer, Charles T.; Conant, Stephanie B.; Hill, Timothy; Gray, Jennifer J.; Niu, Xinnan; Zheng, Mu; Erickson, John J.; Boyd, Kelli L.; McAfee, K. Jill; Oseroff, Carla; Hadrup, Sine R.; Bennink, Jack R.; Hildebrand, William; Edwards, Kathryn M.; Crowe, James E.; Williams, John V.; Buus, Søren; Sette, Alessandro; Schumacher, Ton N.M.; Link, Andrew J.; Joyce, Sebastian

    2013-01-01

    CD8+ T cells (TCD8) confer protective immunity against many infectious diseases, suggesting that microbial TCD8 determinants are promising vaccine targets. Nevertheless, current T cell antigen identification approaches do not discern which epitopes drive protective immunity during active infection — information that is critical for the rational design of TCD8-targeted vaccines. We employed a proteomics-based approach for large-scale discovery of naturally processed determinants derived from a complex pathogen, vaccinia virus (VACV), that are presented by the most frequent representatives of four major HLA class I supertypes. Immunologic characterization revealed that many previously unidentified VACV determinants were recognized by smallpox-vaccinated human peripheral blood cells in a variegated manner. Many such determinants were recognized by HLA class I–transgenic mouse immune TCD8 too and elicited protective TCD8 immunity against lethal intranasal VACV infection. Notably, efficient processing and stable presentation of immune determinants as well as the availability of naive TCD8 precursors were sufficient to drive a multifunctional, protective TCD8 response. Our approach uses fundamental insights into T cell epitope processing and presentation to define targets of protective TCD8 immunity within human pathogens that have complex proteomes, suggesting that this approach has general applicability in vaccine sciences. PMID:23543059

  1. Cutting Edge: Innate Immune Augmenting Vesicular Stomatitis Virus Expressing Zika Virus Proteins Confers Protective Immunity.

    PubMed

    Betancourt, Dillon; de Queiroz, Nina M G P; Xia, Tianli; Ahn, Jeonghyun; Barber, Glen N

    2017-04-15

    Zika virus (ZIKV) has become a serious public health concern because of its link to brain damage in developing human fetuses. Recombinant vesicular stomatitis virus (rVSV) was shown to be a highly effective and safe vector for the delivery of foreign immunogens for vaccine purposes. In this study, we generated rVSVs (wild-type and attenuated VSV with mutated matrix protein [VSVm] versions) that express either the full length ZIKV envelope protein (ZENV) alone or include the ZENV precursor to the membrane protein upstream of the envelope protein, and our rVSV-ZIKV constructs showed efficient immunogenicity in murine models. We also demonstrated maternal protective immunity in challenged newborn mice born to female mice vaccinated with VSVm-ZENV containing the transmembrane domain. Our data indicate that rVSVm may be a suitable strategy for the design of effective vaccines against ZIKV.

  2. International Federation of Library Associations Annual Conference Papers: Libraries Serving the General Public Division: Children's Libraries Section (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Geradts, Alice; And Others

    These seven papers from the section on children's libraries address such topics as library services for children, training of children's librarians, outreach programs for children, bibliographic instruction, and children's literature. Titles of the presentations are "Children's Libraries Information Services to Children, Adults and…

  3. IFLA General Conference, 1992. Division of Libraries Serving the General Public: Open Forum; Section on Libraries Serving Disadvantaged Persons; INTAMEL (RT); Mobile Libraries (RT). Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    Nine papers delivered at the Division of Libraries Serving the General Public at the International Federation of Library Associations and Institutions 1992 annual meeting are presented. In addition to papers about libraries for disadvantaged persons, papers on services to children and young people and to the general public through mobile…

  4. Submergence Confers Immunity Mediated by the WRKY22 Transcription Factor in Arabidopsis[W

    PubMed Central

    Hsu, Fu-Chiun; Chou, Mei-Yi; Chou, Shu-Jen; Li, Ya-Ru; Peng, Hsiao-Ping; Shih, Ming-Che

    2013-01-01

    Transcriptional control plays an important role in regulating submergence responses in plants. Although numerous genes are highly induced during hypoxia, their individual roles in hypoxic responses are still poorly understood. Here, we found that expression of genes that encode members of the WRKY transcription factor family was rapidly and strongly induced upon submergence in Arabidopsis thaliana, and this induction correlated with induction of a large portion of innate immunity marker genes. Furthermore, prior submergence treatment conferred higher resistance to the bacterial pathogen Pseudomonas syringae in Arabidopsis. Among the WRKY genes tested, WRKY22 had the highest level of induction during the early stages of submergence. Compared with the wild type, WRKY22 T-DNA insertion mutants wrky22-1 and wrky22-2 had lower disease resistance and lower induction of innate immunity markers, such as FLG22-INDUCED RECEPTOR-LIKE KINASE1 (FRK1) and WRKY53, after submergence. Furthermore, transcriptomic analyses of wrky22-2 and chromatin immunoprecipitation identified several potential targets of WRKY22, which included genes encoding a TIR domain–containing protein, a plant peptide hormone, and many OLIGO PEPTIDE TRANSPORTER genes, all of which may lead to induction of innate immunity. In conclusion, we propose that submergence triggers innate immunity in Arabidopsis via WRKY22, a response that may protect against a higher probability of pathogen infection either during or after flooding. PMID:23897923

  5. RESET: Information Services Trends in 2012. Selected Papers from PIALA Conference 2012, Pacific Islands Association of Libraries, Archives, and Museums Annual Conference (22nd, Tumon, U.S. Territory of Guam, Nov 13-16, 2012)

    ERIC Educational Resources Information Center

    Drake, Paul B., Ed.

    2013-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 22th annual conference was held in Tumon, Territory of Guam USA, November 13-16, 2012. The volume begins with a listing of the members of the PIALA 2012 Planning Committee and…

  6. "Be Informed, Stay Connected, Community Transformation!" Selected Papers from the PIALA Conference 2014, Pacific Islands Association of Libraries, Archives, and Museums Annual Conference (24th, Koror, Republic of Palau, Nov 10-15, 2014)

    ERIC Educational Resources Information Center

    Drake, Paul Burton

    2015-01-01

    This publication follows the tradition of publishing selected papers from the Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 24th annual conference was held in Koror, Republic of Palau, November 10-15, 2014. This volume includes a listing of the PIALA 2014 Organizing Committee and PIALA Officers and…

  7. Pacific Visions: Finding, Selecting, and Using Resources for Your Libraries, Archives, and Museums. Selected Papers from PIALA 2009, Pacific Islands Association of Libraries, Archives, and Museums Annual Conference (19th, Pohnpei, Federated States of Micronesia, November 16-21, 2009)

    ERIC Educational Resources Information Center

    Drake, Paul B., Ed.

    2011-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 19th annual conference was held in Pohnpei, Federated States of Micronesia, November 16-21, 2009. The volume begins with a listing of the members of the PIALA 2009 Planning…

  8. Discover the Hidden Jewels in Your Library and Sharing the Wealth through Collaboration. Selected Papers from PIALA 2011, Pacific Islands Association of Libraries, Archives, and Museums Annual Conference (21st, Kosrae, Federated States of Micronesia, November 14-17, 2011)

    ERIC Educational Resources Information Center

    Drake, Paul B., Ed.

    2012-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 21st annual conference was held in Kosrae, Federated States of Micronesia, November 14-17, 2011. The volume begins with a listing of the members of the PIALA 2011 Planning…

  9. Classification Schedules as Subject Enhancement in Online Catalogs. A Review of a Conference Sponsored by Forest Press, the OCLC Online Computer Library Center, and the Council on Library Resources.

    ERIC Educational Resources Information Center

    Mandel, Carol A.

    This paper presents a synthesis of the ideas and issues developed at a conference convened to review the results of the Dewey Decimal Classification Online Project and explore the potential for future use of the Dewey Decimal Classification (DDC) and Library of Congress Classification (LCC) schedules in online library catalogs. Conference…

  10. The Future of Libraries in Human Communication: Abstracts and Fulltext Documents of Papers and Demos Given at the [International Association of Technological University Libraries] IATUL Conference (Chania, Greece, May 17-21, 1999). Volume 19.

    ERIC Educational Resources Information Center

    International Association of Technological Univ. Libraries, Gothenburg (Sweden).

    This proceedings of the IATUL 1999 conference of the contains the following papers: "From Industry to Higher Education and Libraries: Building the Fast Response Library" (A. S. Apostolou & C. H. Skiadas); "Managing the Learning Agenda in a Converged Service Environment" (Richard Biddiscombe); "Management of Electronic Information" (Michael…

  11. George Orwell &"Nineteen Eighty-Four": The Man and the Book. A Conference at the Library of Congress, April 30 and May 1, 1984.

    ERIC Educational Resources Information Center

    Library of Congress, Washington, DC.

    Proceedings of a conference of international Orwell experts gathered at the Library of Congress are presented in this collection. The collection is divided into four sections, corresponding with the four conference sessions: "What Orwell Really Wrote"; "Orwell: The Man"; "'Nineteen Eighty-Four': The Book"; and…

  12. IFLA General Conference 1988. Division of Special Libraries. Section on Science and Technology Libraries; Section on Art Libraries; Section on Social Science Libraries; Section on Geography and Map Libraries; Division of Contributed Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 23 papers in this collection include papers on special libraries and miscellaneous contributed papers: (1) "Networking Potentialities and Limitations--Special Library Networks in Socialist Countries--An Overview, and the Main Ways of Perestroika in the Work of Scientific and Technical Libraries at the Present Stage" (D. Schmidmaier…

  13. IFLA General Conference, 1992. Division of Special Libraries: Section on Art Libraries; Section on Geography and Map Libraries; Section on Government Libraries; Section on Science and Technology Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    The following 21 papers were delivered for the Special Libraries Division of the International Federation of Library Associations and Institutions at its 1992 annual meeting: (1) "From Indochina to Afghanistan: Arts from Abroad in Parisian Libraries" (M. F. Macouin); (2) "The Indonesian Archeology Photograph and Documentation System…

  14. IFLA General Conference, 1990. Division of General Research Libraries: Open Forum; Section of National Libraries; Section of University Libraries and Other General Research Libraries; Section of Parliamentary Libraries. Booklet 1.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 16 reports in this collection were presented at an open forum on research libraries and meetings of three more specialized sections: (1) "Nature and Purpose of the Division of General Research Libraries" (Ernst Kohl); (2) "Objectives and Goals of the Section of Parliamentary Libraries" (Ernst Kohl); (3) "The IFLA…

  15. IFLA General Conference 1988. Division of Special Libraries. Section on Science and Technology Libraries; Section on Art Libraries; Section on Social Science Libraries; Section on Geography and Map Libraries; Division of Contributed Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 23 papers in this collection include papers on special libraries and miscellaneous contributed papers: (1) "Networking Potentialities and Limitations--Special Library Networks in Socialist Countries--An Overview, and the Main Ways of Perestroika in the Work of Scientific and Technical Libraries at the Present Stage" (D. Schmidmaier…

  16. IFLA General Conference, 1990. Division of General Research Libraries: Open Forum; Section of National Libraries; Section of University Libraries and Other General Research Libraries; Section of Parliamentary Libraries. Booklet 1.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 16 reports in this collection were presented at an open forum on research libraries and meetings of three more specialized sections: (1) "Nature and Purpose of the Division of General Research Libraries" (Ernst Kohl); (2) "Objectives and Goals of the Section of Parliamentary Libraries" (Ernst Kohl); (3) "The IFLA…

  17. Library and Information Science Education in the Americas: Present and Future. Papers from a Conference held in Austin, Texas, February 14, l980.

    ERIC Educational Resources Information Center

    Jackson, William Vernon, Ed.

    Four primary papers and additional related reaction papers are presented from a conference sponsored by the Association of American Library Schools, the Organization of American States, and the Seminar on the Acquisition of Latin American Library Materials, which was held to promote a useful dialog between educators from North American library…

  18. Canadian Library Association 25th Annual Conference, Hamilton, Ontario, 20-25 June 1970. Proceedings. Theme: Into the Seventies -Challenge of Change.

    ERIC Educational Resources Information Center

    Canadian Library Association, Ottawa (Ontario).

    The fifteen speeches presented at the 25th Annual Conference of the Canadian Library Association are: (1) Presidential Address, (2) Theme Day, (3) The Revolutionary 70's, Are We Ready, (4) The Prime Mover: The Role of the National Library, (5) What You Expect Out of Machines, (6) Twenty-Seven Million People: Four Million Square Miles: Where Shall…

  19. Library and Information Science Education in the Americas: Present and Future. Papers from a Conference held in Austin, Texas, February 14, l980.

    ERIC Educational Resources Information Center

    Jackson, William Vernon, Ed.

    Four primary papers and additional related reaction papers are presented from a conference sponsored by the Association of American Library Schools, the Organization of American States, and the Seminar on the Acquisition of Latin American Library Materials, which was held to promote a useful dialog between educators from North American library…

  20. Proceedings of the Annual Conference, Association for Population/Family Planning Libraries and Information Centers-International (11th, Atlanta, Georgia, April 11-13, 1978).

    ERIC Educational Resources Information Center

    Bergman, Rita F., Ed.

    The major sections of these conference proceedings are (1) what's new worldwide in population information activities; (2) management and libraries; (3) the Select Committee on Population; (4) workshop reports; (5) contributed papers; (6) APLIC-PAA (Association for Population/Family Planning Libraries and Information Centers-Population Association…

  1. Canadian Library Association 25th Annual Conference, Hamilton, Ontario, 20-25 June 1970. Proceedings. Theme: Into the Seventies -Challenge of Change.

    ERIC Educational Resources Information Center

    Canadian Library Association, Ottawa (Ontario).

    The fifteen speeches presented at the 25th Annual Conference of the Canadian Library Association are: (1) Presidential Address, (2) Theme Day, (3) The Revolutionary 70's, Are We Ready, (4) The Prime Mover: The Role of the National Library, (5) What You Expect Out of Machines, (6) Twenty-Seven Million People: Four Million Square Miles: Where Shall…

  2. Summer Conference on International Inter-Library Cooperation Held at Clarkson College of Technology, Potsdam, New York, June 7-9, 1967.

    ERIC Educational Resources Information Center

    North Country Reference and Research Resources Council, Canton, NY.

    The conference was held by the North Country Reference and Research Resources Council as a means of communicating and cooperating with Canadian libraries situated in close proximity to the Northern New York State libraries composing the Council. Topics covered by invited speakers and round table discussions included: (1) cooperative library…

  3. Th17 cells confer long term adaptive immunity to oral mucosal Candida albicans infections

    PubMed Central

    Hernández-Santos, Nydiaris; Huppler, Anna R.; Peterson, Alanna C.; Khader, Shabaana A.; McKenna, Kyle C.; Gaffen, Sarah L.

    2012-01-01

    Oropharyngeal candidiasis (OPC) is an opportunistic infection caused by Candida albicans. Despite its prevalence, little is known about C. albicans-specific immunity in the oral mucosa. Vaccines against Candida generate both Th1 and Th17 responses, and considerable evidence implicates IL-17 in immunity to OPC. However, IL-17 is also produced by innate immune cells that are remarkably similar to Th17 cells, expressing the same markers and localizing to similar mucosal sites. To date, the relative contribution(s) of Th1, Th17 and innate IL-17-producing cells in OPC have not been clearly defined. Here, we sought to determine the nature and function of adaptive T cell responses to OPC, using a new recall infection model. Mice subjected to infection and re-challenge with Candida mounted a robust and stable antigen specific IL-17 response in CD4+ but not CD8+ T cells. There was little evidence for Th1 or Th1/Th17 responses. The Th17 response promoted accelerated fungal clearance, and Th17 cells could confer protection in Rag1−/− mice upon adoptive transfer. Surprisingly, CD4 deficiency did not cause OPC, but was instead associated with compensatory IL-17 production by Tc17 and CD4-CD8-CD3+ cells. Therefore, classic CD4+Th17 cells protect from OPC, but can be compensated by other IL-17-producing cells in CD4-deficient hosts. PMID:23250275

  4. IFLA General Conference, 1990. IFLA Presession Seminar on Public Library Policy.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Five of the six papers in this collection focus on public library services, collection development problems, and involvement in literacy campaigns in developing countries; the sixth paper is concerned with public library services in Norway. (1) In "Big Cities, Its Inhabitants and Public Libraries Systems," May Brooking Negrao examines…

  5. Immunization with plasmid DNA encoding the hemagglutinin and the nucleoprotein confers robust protection against a lethal canine distemper virus challenge.

    PubMed

    Dahl, Lotte; Jensen, Trine Hammer; Gottschalck, Elisabeth; Karlskov-Mortensen, Peter; Jensen, Tove Dannemann; Nielsen, Line; Andersen, Mads Klindt; Buckland, Robin; Wild, T Fabian; Blixenkrone-Møller, Merete

    2004-09-09

    We have investigated the protective effect of immunization of a highly susceptible natural host of canine distemper virus (CDV) with DNA plasmids encoding the viral nucleoprotein (N) and hemagglutinin (H). The combined intradermal and intramuscular routes of immunization elicited high virus-neutralizing serum antibody titres in mink (Mustela vison). To mimic natural exposure, we also conducted challenge infection by horizontal transmission from infected contact animals. Other groups received a lethal challenge infection by administration to the mucosae of the respiratory tract and into the muscle. One of the mink vaccinated with N plasmid alone developed severe disease after challenge. In contrast, vaccination with the H plasmid together with the N plasmid conferred solid protection against disease and we were unable to detect CDV infection in PBMCs or in different tissues after challenge. Our findings show that DNA immunization by the combined intradermal and intramuscular routes can confer solid protective immunity against naturally transmitted morbillivirus infection and disease.

  6. IFLA General Conference, 1987. Division of Libraries Serving the General Public. Libraries Serving Disadvantaged Persons Section. Library Services to Multicultural Populations Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Eleven of the 15 papers in this collection discuss library services for disadvantaged persons; the remaining four papers are concerned with services to multicultural populations. The papers from the Section of Libraries Serving Disadvantaged Persons appear first in this list: (1) "The Development and Future of Easy Readers for Adults in the UK"…

  7. IFLA General Conference, 1989. Division of Special Libraries. Section of Administrative Libraries; Section of Social Science Libraries; Section of Geography and Map Libraries; Section of Science and Technology Libraries; Section of Biological and Medical Science Libraries; Section of Art Libraries; Joint Session with the Round Table on Audiovisual Media. The International Association of Law Libraries. Booklet 20.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Twenty-one papers from the Division of Special Libraries are included in this collection: "Information Systems Planning as a Tool of Developing Library Work: The Case of Statistics Library" (Heli Myllys); "The Libraries of the French Central Government Departments and the Administrative Library of the City of Paris" (French and English versions;…

  8. IFLA General Conference, 1989. Division of Special Libraries. Section of Administrative Libraries; Section of Social Science Libraries; Section of Geography and Map Libraries; Section of Science and Technology Libraries; Section of Biological and Medical Science Libraries; Section of Art Libraries; Joint Session with the Round Table on Audiovisual Media. The International Association of Law Libraries. Booklet 20.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Twenty-one papers from the Division of Special Libraries are included in this collection: "Information Systems Planning as a Tool of Developing Library Work: The Case of Statistics Library" (Heli Myllys); "The Libraries of the French Central Government Departments and the Administrative Library of the City of Paris" (French and English versions;…

  9. Library+

    ERIC Educational Resources Information Center

    Merrill, Alex

    2011-01-01

    This article discusses possible future directions for academic libraries in the post Web/Library 2.0 world. These possible directions include areas such as data literacy, linked data sets, and opportunities for libraries in support of digital humanities. The author provides a brief sketch of the background information regarding the topics and…

  10. Immunization of mice with a novel recombinant molecular chaperon confers protection against Brucella melitensis infection.

    PubMed

    Ghasemi, Amir; Jeddi-Tehrani, Mahmood; Mautner, Josef; Salari, Mohammad Hossein; Zarnani, Amir-Hassan

    2014-11-20

    Brucella spp. are zoonotic Gram-negative intracellular pathogens with the ability to survive and replicate in phagocytes. It has been shown that bacterial proteins expressed abundantly in this niche are stress-related proteins capable of triggering effective immune responses. BMEI1549 is a molecular chaperone designated DnaK that is expressed under stress conditions and helps to prevent formation of protein aggregates. In order to study the potential of DnaK as a prospective Brucella subunit vaccine, immunogenicity and protective efficacy of recombinant DnaK from Brucella melitensis was evaluated in BALB/c mice. The dnak gene was cloned, expressed in Escherichia coli, and the resulting recombinant protein used as subunit vaccine. DnaK-immunized mice showed a strong lymphocyte proliferative response to in vitro antigen stimulation. Although comparable levels of antigen-specific IgG2a and IgG1 were observed in immunized mice, high amounts of IFN-γ, IL-12 and IL-6, no detectable level of IL-4 and very low levels of IL-10 and IL-5 were produced by splenocytes of vaccinated mice suggesting induction of a Th1 dominant immune response by DnaK. Compared to control animals, mice vaccinated with DnaK exhibited a significant degree of protection against subsequent Brucella infection (p<0.001), albeit this protection was less than the protection conferred by Rev.1 (p<0.05). A further increase in protection was observed, when DnaK was combined with recombinant Omp31. Notably, this combination, as opposed to each component alone, induced statistically similar level of protection as induced by Rev.1 suggesting that DnaK could be viewed as a promising candidate for the development of a subunit vaccine against brucellosis.

  11. Writer to Writer: How To Conference Young Authors. The Bill Harp Professional Teachers Library Series.

    ERIC Educational Resources Information Center

    Thomason, Tommy

    Written for the busy teacher, this book examines classroom writing conferences, one of the most talked about and least practiced strategies in the writing process classroom. Each brief chapter introduces a different facet of the writing conference or the philosophy and classroom practice that undergird it, and each chapter ends with a…

  12. Writer to Writer: How To Conference Young Authors. The Bill Harp Professional Teachers Library Series.

    ERIC Educational Resources Information Center

    Thomason, Tommy

    Written for the busy teacher, this book examines classroom writing conferences, one of the most talked about and least practiced strategies in the writing process classroom. Each brief chapter introduces a different facet of the writing conference or the philosophy and classroom practice that undergird it, and each chapter ends with a…

  13. Tetanus Neurotoxin Neutralizing Antibodies Screened from a Human Immune scFv Antibody Phage Display Library

    PubMed Central

    Wang, Han; Yu, Rui; Fang, Ting; Yu, Ting; Chi, Xiangyang; Zhang, Xiaopeng; Liu, Shuling; Fu, Ling; Yu, Changming; Chen, Wei

    2016-01-01

    Tetanus neurotoxin (TeNT) produced by Clostridium tetani is one of the most poisonous protein substances. Neutralizing antibodies against TeNT can effectively prevent and cure toxicosis. Using purified Hc fragments of TeNT (TeNT-Hc) as an antigen, three specific neutralizing antibody clones recognizing different epitopes were selected from a human immune scFv antibody phage display library. The three antibodies (2-7G, 2-2D, and S-4-7H) can effectively inhibit the binding between TeNT-Hc and differentiated PC-12 cells in vitro. Moreover, 2-7G inhibited TeNT-Hc binding to the receptor via carbohydrate-binding sites of the W pocket while 2-2D and S-4-7H inhibited binding of the R pocket. Although no single mAb completely protected mice from the toxin, they could both prolong survival when challenged with 20 LD50s (50% of the lethal dose) of TeNT. When used together, the mAbs completely neutralized 1000 LD50s/mg Ab, indicating their high neutralizing potency in vivo. Antibodies recognizing different carbohydrate-binding pockets could have higher synergistic toxin neutralization activities than those that recognize the same pockets. These results could lead to further production of neutralizing antibody drugs against TeNT and indicate that using TeNT-Hc as an antigen for screening human antibodies for TeNT intoxication therapy from human immune antibody library was convenient and effective. PMID:27626445

  14. Tetanus Neurotoxin Neutralizing Antibodies Screened from a Human Immune scFv Antibody Phage Display Library.

    PubMed

    Wang, Han; Yu, Rui; Fang, Ting; Yu, Ting; Chi, Xiangyang; Zhang, Xiaopeng; Liu, Shuling; Fu, Ling; Yu, Changming; Chen, Wei

    2016-09-11

    Tetanus neurotoxin (TeNT) produced by Clostridium tetani is one of the most poisonous protein substances. Neutralizing antibodies against TeNT can effectively prevent and cure toxicosis. Using purified Hc fragments of TeNT (TeNT-Hc) as an antigen, three specific neutralizing antibody clones recognizing different epitopes were selected from a human immune scFv antibody phage display library. The three antibodies (2-7G, 2-2D, and S-4-7H) can effectively inhibit the binding between TeNT-Hc and differentiated PC-12 cells in vitro. Moreover, 2-7G inhibited TeNT-Hc binding to the receptor via carbohydrate-binding sites of the W pocket while 2-2D and S-4-7H inhibited binding of the R pocket. Although no single mAb completely protected mice from the toxin, they could both prolong survival when challenged with 20 LD50s (50% of the lethal dose) of TeNT. When used together, the mAbs completely neutralized 1000 LD50s/mg Ab, indicating their high neutralizing potency in vivo. Antibodies recognizing different carbohydrate-binding pockets could have higher synergistic toxin neutralization activities than those that recognize the same pockets. These results could lead to further production of neutralizing antibody drugs against TeNT and indicate that using TeNT-Hc as an antigen for screening human antibodies for TeNT intoxication therapy from human immune antibody library was convenient and effective.

  15. Inaugural conference of the International Association of Immunization Managers (IAIM), Istanbul Turkey, 3-4 March 2015.

    PubMed

    Carrasco, Peter; Franco-Paredes, Carlos; Andrus, Jon; Waller, Katie; Maassen, Alison; Symenouh, Emi; Hafalia, Gabrielle

    2015-08-07

    For more than 35 years, most national immunization programs have established managerial structures and processes for delivering vaccination services to their populations. These days, immunization managers are facing an increasing number of challenges due to the introduction of new vaccines, shifting demographic patterns, complex networks of service providers, and maintaining the gains achieved with previous vaccination efforts. To confront these challenges, better program performance will require better managerial practices, which incorporates new technologies. To that end, the International Association of Immunization Managers (IAIM) is the first global professional association launched to promote superior leadership and management skills among health professionals involved with vaccination efforts worldwide. From 3 to 4 March 2015, approximately 132 members from 70 countries representing six regions, gathered in Istanbul, Turkey for the inaugural conference of IAIM. In the two-day program, members selected thirteen peers to constitute the Governing Council. The 12 articles of the bylaws of the Association were also ratified. This conference was a forum for sharing managerial best practices through networking sessions, breakout sessions, and presentations. Members also learned about IAIM sponsored training opportunities to deepen their managerial competencies through peer-to-peer exchanges and scholarship training programs. We believe that the IAIM inaugural conference was an appropriate platform for equipping managers with tools and professional network of peers to support them in achieving national, regional and global immunization goals, including those of the Global Vaccine Action Plan of the World Health Organization. Copyright © 2015. Published by Elsevier Ltd.. All rights reserved.

  16. Personal, Electronic, Secure National Library of Medicine Hosts Health Records Conference

    MedlinePlus

    ... Bar Home Current Issue Past Issues EHR Personal, Electronic, Secure: National Library of Medicine Hosts Health Records ... One suggestion for saving money is to implement electronic personal health records. With this in mind, the ...

  17. Immune suppressor factor confers stromal cell line with enhanced supporting activity for hematopoietic stem cells

    SciTech Connect

    Nakajima, Hideaki . E-mail: hnakajim@ims.u-tokyo.ac.jp; Shibata, Fumi; Fukuchi, Yumi; Goto-Koshino, Yuko; Ito, Miyuki; Urano, Atsushi; Nakahata, Tatsutoshi; Aburatani, Hiroyuki; Kitamura, Toshio

    2006-02-03

    Immune suppressor factor (ISF) is a subunit of the vacuolar ATPase proton pump. We earlier identified a short form of ISF (ShIF) as a stroma-derived factor that supports cytokine-independent growth of mutant Ba/F3 cells. Here, we report that ISF/ShIF supports self-renewal and expansion of primary hematopoietic stem cells (HSCs). Co-culture of murine bone marrow cells with a stromal cell line overexpressing ISF or ShIF (MS10/ISF or MS10/ShIF) not only enhanced their colony-forming activity and the numbers of long-term culture initiating cells, but also maintained the competitive repopulating activity of HSC. This stem cell supporting activity depended on the proton-transfer function of ISF/ShIF. Gene expression analysis of ISF/ShIF-transfected cell lines revealed down-regulation of secreted frizzled-related protein-1 and tissue inhibitor of metalloproteinase-3, and the restoration of their expressions in MS10/ISF cells partially reversed its enhanced LTC-IC supporting activity to a normal level. These results suggest that ISF/ShIF confers stromal cells with enhanced supporting activities for HSCs by modulating Wnt-activity and the extracellular matrix.

  18. Lactobacillus plantarum DK119 as a Probiotic Confers Protection against Influenza Virus by Modulating Innate Immunity

    PubMed Central

    Park, Min-Kyung; NGO, Vu; Kwon, Young-Man; Lee, Young-Tae; Yoo, Sieun; Cho, Young-Hee; Hong, Sung-Moon; Hwang, Hye Suk; Ko, Eun-Ju; Jung, Yu-Jin; Moon, Dae-Won; Jeong, Eun-Ji; Kim, Min-Chul; Lee, Yu-Na; Jang, Ji-Hun; Oh, Joon-Suk; Kim, Cheol-Hyun; Kang, Sang-Moo

    2013-01-01

    Lactobacillus plantarum DK119 (DK119) isolated from the fermented Korean cabbage food was used as a probiotic to determine its antiviral effects on influenza virus. DK119 intranasal or oral administration conferred 100% protection against subsequent lethal infection with influenza A viruses, prevented significant weight loss, and lowered lung viral loads in a mouse model. The antiviral protective efficacy was observed in a dose and route dependent manner of DK119 administration. Mice that were treated with DK119 showed high levels of cytokines IL-12 and IFN-γ in bronchoalveolar lavage fluids, and a low degree of inflammation upon infection with influenza virus. Depletion of alveolar macrophage cells in lungs and bronchoalveolar lavages completely abrogated the DK119-mediated protection. Modulating host innate immunity of dendritic and macrophage cells, and cytokine production pattern appeared to be possible mechanisms by which DK119 exhibited antiviral effects on influenza virus infection. These results indicate that DK119 can be developed as a beneficial antiviral probiotic microorganism. PMID:24124485

  19. Lactobacillus plantarum DK119 as a probiotic confers protection against influenza virus by modulating innate immunity.

    PubMed

    Park, Min-Kyung; Ngo, Vu; Kwon, Young-Man; Lee, Young-Tae; Yoo, Sieun; Cho, Young-Hee; Hong, Sung-Moon; Hwang, Hye Suk; Ko, Eun-Ju; Jung, Yu-Jin; Moon, Dae-Won; Jeong, Eun-Ji; Kim, Min-Chul; Lee, Yu-Na; Jang, Ji-Hun; Oh, Joon-Suk; Kim, Cheol-Hyun; Kang, Sang-Moo

    2013-01-01

    Lactobacillus plantarum DK119 (DK119) isolated from the fermented Korean cabbage food was used as a probiotic to determine its antiviral effects on influenza virus. DK119 intranasal or oral administration conferred 100% protection against subsequent lethal infection with influenza A viruses, prevented significant weight loss, and lowered lung viral loads in a mouse model. The antiviral protective efficacy was observed in a dose and route dependent manner of DK119 administration. Mice that were treated with DK119 showed high levels of cytokines IL-12 and IFN-γ in bronchoalveolar lavage fluids, and a low degree of inflammation upon infection with influenza virus. Depletion of alveolar macrophage cells in lungs and bronchoalveolar lavages completely abrogated the DK119-mediated protection. Modulating host innate immunity of dendritic and macrophage cells, and cytokine production pattern appeared to be possible mechanisms by which DK119 exhibited antiviral effects on influenza virus infection. These results indicate that DK119 can be developed as a beneficial antiviral probiotic microorganism.

  20. White House Conference on Library and Information Services. Hearing before the Subcommittee on Education, Arts and Humanities of the Committee on Labor and Human Resources. United States Senate, One Hundredth Congress, First Session.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. Senate Committee on Labor and Human Resources.

    Held to authorize a White House Conference on Library and Information Services (WHCLIST) in 1989, this hearing focused on library services for literacy, productivity, and democracy as well as on the conference structure and costs. Statements and supporting information provided by the following individuals and institutions were presented: (1)…

  1. Culture Keepers III: Making Global Connections. Proceedings of the Black Caucus of the American Library Association (BCALA) National Conference of African American Librarians (3rd, Winston-Salem, North Carolina, July 31-August 3, 1997).

    ERIC Educational Resources Information Center

    Neely, Teresa Y., Ed.

    The 49 papers presented in this volume are evidence of the research, scholarship, and professional nature of the offerings of the third National Conference of African American Librarians, sponsored by the Black Caucus of the American Library Association. The papers are arranged into nine tracks and Pre-Conference topics: I: Library Connections: An…

  2. Mucosal and systemic immunization with a novel attenuated pneumococcal vaccine candidate confer serotype independent protection against Streptococcus pneumoniae in mice.

    PubMed

    Wu, Kaifeng; Yao, Run; Wang, Hong; Pang, Dan; Liu, Yusi; Xu, Hongmei; Zhang, Shuai; Zhang, Xuemei; Yin, Yibing

    2014-07-16

    Despite the availability of effective vaccines, Streptococcus pneumoniae is still one of the major infectious diseases causing substantial morbidity and mortality in children under 5 years old. In this study, we demonstrate the protective efficacy of S. pneumoniae SPY1, a novel live attenuated vaccine strain against pneumococcal infection in murine models. This strain was characterized by defects in three important pneumococcal virulence factors including capsule, teichoic acids and pneumolysin. The lactate dehydrogenase assays and in vivo animal experiments demonstrated a significantly attenuated virulence and a reduced nasopharyngeal colonization for the SPY1 strain. We also show that mucosal and systemic immunization with the live SPY1 strain induced protective immune responses against pneumococci. Mucosal immunization with SPY1 offered better protection against colonization challenge with strains TIGR4 and serotype 19F than systemic SPY1 immunization. In invasive infection models, mucosal vaccination with the SPY1 strain conferred complete protection against D39 and clinical serotype 6B and 3 strains. Notably, intranasal vaccination with the SPY1 strain conferred superior protection against pneumococcal invasive disease compared with the commercial available vaccines. SPY1 strain was shown to elicit high levels of serotype-independent antibodies and a mixed cellular immune response. Besides, the SPY1 serum was able to passively protect mice against invasive challenge with D39 strain, indicating the protective effect of the antibody-mediated responses. Together, the SPY1 strain may be a promising live vaccine strain to protect pneumococcal infection.

  3. IFLA General Conference, 1991. Division of Special Libraries Services: Section of Social Science Libraries; Section of Geography and Map Libraries; Section of Biological and Medical Sciences Libraries; Section of Art Libraries. Booklet 2.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    The 10 papers in this booklet were presented at meetings of 4 sections within the Division of Special Libraries: (1) "Information Ensurance of a Scientist" (V. Matveyev, USSR); (2) "Linguistic Barriers and Machine Translation" (Stanley Kalkus, USA); (3) "Maps for Planning" (V. I. Zhukov and L. G. Rudenko, USSR); (4)…

  4. IFLA General Conference, 1991. Division of Special Libraries Services: Section of Social Science Libraries; Section of Geography and Map Libraries; Section of Biological and Medical Sciences Libraries; Section of Art Libraries. Booklet 2.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    The 10 papers in this booklet were presented at meetings of 4 sections within the Division of Special Libraries: (1) "Information Ensurance of a Scientist" (V. Matveyev, USSR); (2) "Linguistic Barriers and Machine Translation" (Stanley Kalkus, USA); (3) "Maps for Planning" (V. I. Zhukov and L. G. Rudenko, USSR); (4)…

  5. Laser Disks at the Library Door: The Microsoft First International Conference on CD-ROM.

    ERIC Educational Resources Information Center

    Miller, David C.

    1986-01-01

    Description of First International CD-ROM Conference held by Microsoft, Inc., in March, 1986, covers types of participants, hardware and support services, and technical standards and intellectual property rights as market barriers. The importance of librarians planning for CD-ROM is stressed, and a detailed directory of references and contacts is…

  6. Libraries, Archives and Museums: What's in Them for Us? PIALA '98. Selected Papers from the Pacific Islands Association of Libraries and Archives Conference (8th, Tofol, Kosrae, Federated States of Micronesia, November 17-20, 1998).

    ERIC Educational Resources Information Center

    Cohen, Arlene, Ed.

    This proceedings contains papers from the 1998 annual conference of the Pacific Islands Association of Libraries and Archives (PIALA). After welcoming remarks from Henry Robert and Isabel Rungrad, the following papers are included: "Sharing Our Successes, Discussing Our Future: A Survey of Pacific Collections Activities--Report from the…

  7. New Beginnings: The Library as an Information and Resource Basket. Selected Papers from PIALA 2008: Pacific Islands Association of Libraries, Archives and Museums Annual Conference (18th, Colonia, Yap, Federated States of Micronesia, November 17-21, 2001)

    ERIC Educational Resources Information Center

    Horie, Ruth H., Ed.

    2009-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. The volume begins with a listing of the members of the PIALA 2008 Planning Committee, the Officers and Executive Board, and Acknowledgements, followed by the Schedule of Events and a…

  8. The neutralizing human recombinant antibodies to pathogenic Orthopoxviruses derived from a phage display immune library.

    PubMed

    Tikunova, Nina; Dubrovskaya, Viktoriya; Morozova, Vera; Yun, Tatiana; Khlusevich, Yana; Bormotov, Nikolai; Laman, Aleksandr; Brovko, Fedor; Shvalov, Aleksandr; Belanov, Eugeni

    2012-01-01

    A panel of recombinant human antibodies to orthopoxviruses was isolated from a combinatorial phage display library of human scFv antibodies constructed from the Vh and Vl genes cloned from the peripheral blood lymphocytes of Vaccinia virus (VACV) immune donors. Plaque-reduction neutralization tests showed that seven selected phage-displaying scFv antibodies (pdAbs) neutralized both CPXV and VACV, and five of them neutralized Monkeypox virus (MPXV). Western blot analysis of VACV and CPXV proteins demonstrated that seven neutralizing antibodies recognized a 35 kDa protein. To identify this target protein, we produced a recombinant J3L protein of CPXV and showed that all the selected neutralizing antibodies recognized this protein. Neutralizing pdAb b9 was converted into fully human mAb b9 (fh b9), and scFv b9 displayed high binding affinities (K(d) of 0.7 and 3.2 nM). The fh b9 reduced VACV plaque formation in a dose-dependent manner.

  9. Preservation Integrity of Our Nation's Records at Risk. White House Conference on Library and Information Services.

    ERIC Educational Resources Information Center

    Fusonie, Alan

    This paper focuses on the preservation of documents that are disintegrating rapidly in national archives. After providing an introduction and background to the situation, the paper outlines a national preservation strategy for administrators and discusses ways to protect collections in archives and libraries throughout the United States. The…

  10. Marketing: Putting People in the Process. White House Conference on Library and Information Services.

    ERIC Educational Resources Information Center

    Leisner, Anthony

    Noting that some public, school, law, academic, and special libraries have adopted marketing as a management tool, this paper begins by discussing marketing principles as defined by the American Marketing Association (AMA). Librarians are advised to center their marketing activities around what is known as the "4 P's" of marketing:…

  11. Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.

    PubMed

    Le Luduec, Jean-Benoît; Debeer, Sabine; Piras, Fabienne; Andréoni, Christine; Boudet, Florence; Laurent, Philippe; Kaiserlian, Dominique; Dubois, Bertrand

    2016-02-10

    Intradermal (ID) vaccination constitutes a promising approach to induce anti-infectious immunity. This route of immunization has mostly been studied with influenza split-virion vaccines. However, the efficacy of ID vaccination for sub-unit vaccines in relation to underlying skin innate immunity remains to be explored for wider application in humans. Relevant animal models that more closely mimic human skin immunity than the widely used mouse models are therefore necessary. Here, we show in domestic swine, which shares striking anatomic and functional properties with human skin, that a single ID delivery of pseudorabies virus (PRV) glycoproteins without added adjuvant is sufficient to trigger adaptive cellular and humoral immune responses, and to confer protection from a lethal respiratory infection with PRV. Analysis of early events at the skin injection site revealed up-regulation of pro-inflammatory cytokine and chemokine genes, recruitment of neutrophils and monocytes and accumulation of inflammatory DC. We further show that the sustained induction of pro-inflammatory cytokine genes results from the combined effects of skin puncture, liquid injection in the dermis and viral antigens. These data highlight that immune protection against respiratory infection can be induced by ID vaccination with a subunit vaccine and reveal that adjuvant requirements are circumvented by the mechanical and antigenic stress caused by ID injection, which triggers innate immunity and mobilization of inflammatory DC at the immunization site. ID vaccination with sub-unit vaccines may thus represent a safe and efficient solution for protection against respiratory infections in swine and possibly also in humans, given the similarity of skin structure and function in both species.

  12. White House Conference on Library and Information Services: Keynote Addresses, Remarks, and Presented Papers (2nd, Washington, D.C., July 9-13, 1991).

    ERIC Educational Resources Information Center

    National Commission on Libraries and Information Science, Washington, DC.

    This collection of addresses, remarks, and papers presented at the second White House Conference on Library and Information Services (WHCLIS2) includes excerpts from remarks made by First Lady Barbara Bush, President George Bush, Marilyn Quayle, and William T. Esrey of United Telecommunications, Inc., and keynote addresses by Mary Futrell from the…

  13. SLA 2002: Putting Knowledge to Work. Papers Presented at the Special Libraries Association Conference (Los Angeles, California, June 9-12, 2002).

    ERIC Educational Resources Information Center

    Special Libraries Association, Washington, DC.

    This document contains the following papers from the 2002 Special Libraries Association Conference: (1) "Competencies for the 21st Century Information Professional: Translating the SLA Competencies into Business Competencies" (Sue Henczel); (2) "Compromises along the Way: Balancing Speed to Market with Sustainability While…

  14. Library Resources for College Scholars. Transactions of a Conference Held at Washington and Lee University (Lexington, Virginia, February 14-15, 1980).

    ERIC Educational Resources Information Center

    Danford, Robert E., Ed.

    Convened by representatives of 15 private liberal arts colleges, this conference addressed the role and place of the library in the liberal arts college, the needs of academic users, and alternatives to current practices. This collection of presentations and reports includes "Introductory Remarks," by Robert E. R. Huntley; "Collection Development…

  15. IFLA General Conference, 1985. International Association of Law Libraries (IALL) Round-Table on Access to Information in International Legal Research. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on international access to information pertaining to legal research, which were presented at the 1985 conference of the International Federation of Library Associations (IFLA) include: (1) "Materials of European Intergovernmental Organizations and Their Accessibility through Available Research Tools" (Irene Berkey, Northwestern…

  16. Energies for Transition. Proceedings of the National Conference of the Association of College and Research Libraries (4th, Baltimore, Maryland, April 9-12, 1986).

    ERIC Educational Resources Information Center

    Nitecki, Danuta A., Ed.

    This proceedings of the 1986 conference of the Association of College and Research Libraries contains 60 papers--44 position papers, 7 research reports, and 9 idea briefs--dealing with the issue of transitions facing librarians and ways to respond to these transitions through both formal and informal means. Papers are organized under eight broad…

  17. Proceedings of the Fourth National Conference on Population/Family Planning Library and Information Services, Chapel Hill, N.C., May 6-7, 1971.

    ERIC Educational Resources Information Center

    Horowitz, Blanche, Ed.

    This report contains the proceedings of the Fourth National Conference on Population/Family Planning Library and Information Services held in Chapel Hill, North Carolina, May 6-7, 1971. Under the joint sponsorship of the Carolina Population Center and the U.S. Agency for International Development, this was the fourth in a series of annual…

  18. PIALA '97. Wasahn Kamarain: Place of Enlightenment. Papers from the Pacific Islands Association of Libraries and Archives Conference (7th, Palikir, Pohnpei, November 3-5, 1997).

    ERIC Educational Resources Information Center

    Cohen, Arlene, Ed.

    This PIALA 1997 Proceedings follows the tradition of publishing papers from each annual conference of the Pacific Islands Association of Libraries and Archives (PIALA). After welcoming remarks from Sue Moses, Kapily Capelle, and Isabel Rungrad, the following papers are included: "Places of Enlightenment, Places of Dreams" (Maradel Gale);…

  19. Proceedings of the Northumbria International Conference on Performance Measurement in Libraries and Information Services: "Meaningful Measures for Emerging Realities" (4th, Pittsburgh, Pennsylvania, August 12-16, 2001).

    ERIC Educational Resources Information Center

    Stein, Joan, Ed.; Kyrillidou, Martha, Ed.; Davis, Denise, Ed.

    This Fourth Northumbria International Conference on Performance Measurement in Libraries and Information Services centered on the theme of "meaningful measures for emerging realities" and contributors surveyed the field of performance measurement from that perspective. The proceedings begins with seven keynote and invited papers from…

  20. International Federation of Library Associations Annual Conference Papers. Bibliographic Control Division: Bibliography and Cataloguing Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Vita, Susan H.; And Others

    This set of papers delivered to the Bibliographic Control Division of the International Federation of Library Associations (IFLA) during its 47th annual conference (1981) includes: "Cataloging in Publication in the United States--Problems and Prospects," by Susan H. Vita; "Development and Coordination of Bibliographic Activities:…

  1. Proceeding of the ACM/IEEE-CS Joint Conference on Digital Libraries (1st, Roanoke, Virginia, June 24-28, 2001).

    ERIC Educational Resources Information Center

    Association for Computing Machinery, New York, NY.

    Papers in this Proceedings of the ACM/IEEE-CS Joint Conference on Digital Libraries (Roanoke, Virginia, June 24-28, 2001) discuss: automatic genre analysis; text categorization; automated name authority control; automatic event generation; linked active content; designing e-books for legal research; metadata harvesting; mapping the…

  2. IFLA General Conference, 1985. International Association of Law Libraries (IALL) Round-Table on Access to Information in International Legal Research. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on international access to information pertaining to legal research, which were presented at the 1985 conference of the International Federation of Library Associations (IFLA) include: (1) "Materials of European Intergovernmental Organizations and Their Accessibility through Available Research Tools" (Irene Berkey, Northwestern…

  3. Proceedings of the Binational Conference on Libraries in California and Baja California (1st, Tijuana, Baja California, Mexico, January 13-14, 1984) = Memorias de la Primera Conferencia Binacional de Bibliotecas de las Californias.

    ERIC Educational Resources Information Center

    Ayala, Marta Stiefel, Ed.; And Others

    This document includes the text of presentations given at the First Binational Conference on Libraries in California and Baja California, as well as minutes from four roundtables held at the conference. Following a prologue and a brief background on the conference, the following presentations are included: (1) "State Support for Public…

  4. TolC plays a crucial role in immune protection conferred by Edwardsiella tarda whole-cell vaccines

    PubMed Central

    Wang, Chao; Peng, Bo; Li, Hui; Peng, Xuan-xian

    2016-01-01

    Although vaccines developed from live organisms have better efficacy than those developed from dead organisms, the mechanisms underlying this differential efficacy remain unexplored. In this study, we combined sub-immunoproteomics with immune challenge to investigate the action of the outer membrane proteome in the immune protection conferred by four Edwardsiella tarda whole-cell vaccines prepared via different treatments and to identify protective immunogens that play a key role in this immune protection. Thirteen spots representing five outer membrane proteins and one cytoplasmic protein were identified, and it was found that their abundance was altered in relation with the immune protective abilities of the four vaccines. Among these proteins, TolC and OmpA were found to be the key immunogens conferring the first and second highest degrees of protection, respectively. TolC was detected in the two effective vaccines (live and inactivated-30-F). The total antiserum and anti-OmpA titers were higher for the two effective vaccines than for the two ineffective vaccines (inactivated-80-F and inactivated-100). Further evidence demonstrated that the live and inactivated-30-F vaccines demonstrated stronger abilities to induce CD8+ and CD4+ T cell differentiation than the other two evaluated vaccines. Our results indicate that the outer membrane proteome changes dramatically following different treatments, which contributes to the effectiveness of whole-cell vaccines. PMID:27406266

  5. LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis

    PubMed Central

    Silveira, Marcelle M.; Félix, Samuel; Mendonça, Karla S.; Santos, Cleiton S.; Athanazio, Daniel A.; Medeiros, Marco A.; Reis, Mitermayer G.

    2017-01-01

    Neglected tropical diseases, including zoonoses such as leptospirosis, have a major impact on rural and poor urban communities, particularly in developing countries. This has led to major investment in antipoverty vaccines that focus on diseases that influence public health and thereby productivity. While the true, global, impact of leptospirosis is unknown due to the lack of adequate laboratory diagnosis, the WHO estimates that incidence has doubled over the last 15 years to over 1 million cases that require hospitalization every year. Leptospirosis is caused by pathogenic Leptospira spp. and is spread through direct contact with infected animals, their urine or contaminated water and soil. Inactivated leptospirosis vaccines, or bacterins, are approved in only a handful of countries due to the lack of heterologous protection (there are > 250 pathogenic Leptospira serovars) and the serious side-effects associated with vaccination. Currently, research has focused on recombinant vaccines, a possible solution to these problems. However, due to a lack of standardised animal models, rigorous statistical analysis and poor reproducibility, this approach has met with limited success. We evaluated a subunit vaccine preparation, based on a conserved region of the leptospiral immunoglobulin-like B protein (LigB(131–645)) and aluminium hydroxide (AH), in the hamster model of leptospirosis. The vaccine conferred significant protection (80.0–100%, P < 0.05) against mortality in vaccinated animals in seven independent experiments. The efficacy of the LigB(131–645)/AH vaccine ranged from 87.5–100% and we observed sterile immunity (87.5–100%) among the vaccinated survivors. Significant levels of IgM and IgG were induced among vaccinated animals, although they did not correlate with immunity. A mixed IgG1/IgG2 subclass profile was associated with the subunit vaccine, compared to the predominant IgG2 profile seen in bacterin vaccinated hamsters. These findings suggest that

  6. LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis.

    PubMed

    Conrad, Neida L; Cruz McBride, Flávia W; Souza, Jéssica D; Silveira, Marcelle M; Félix, Samuel; Mendonça, Karla S; Santos, Cleiton S; Athanazio, Daniel A; Medeiros, Marco A; Reis, Mitermayer G; Dellagostin, Odir A; McBride, Alan J A

    2017-03-01

    Neglected tropical diseases, including zoonoses such as leptospirosis, have a major impact on rural and poor urban communities, particularly in developing countries. This has led to major investment in antipoverty vaccines that focus on diseases that influence public health and thereby productivity. While the true, global, impact of leptospirosis is unknown due to the lack of adequate laboratory diagnosis, the WHO estimates that incidence has doubled over the last 15 years to over 1 million cases that require hospitalization every year. Leptospirosis is caused by pathogenic Leptospira spp. and is spread through direct contact with infected animals, their urine or contaminated water and soil. Inactivated leptospirosis vaccines, or bacterins, are approved in only a handful of countries due to the lack of heterologous protection (there are > 250 pathogenic Leptospira serovars) and the serious side-effects associated with vaccination. Currently, research has focused on recombinant vaccines, a possible solution to these problems. However, due to a lack of standardised animal models, rigorous statistical analysis and poor reproducibility, this approach has met with limited success. We evaluated a subunit vaccine preparation, based on a conserved region of the leptospiral immunoglobulin-like B protein (LigB(131-645)) and aluminium hydroxide (AH), in the hamster model of leptospirosis. The vaccine conferred significant protection (80.0-100%, P < 0.05) against mortality in vaccinated animals in seven independent experiments. The efficacy of the LigB(131-645)/AH vaccine ranged from 87.5-100% and we observed sterile immunity (87.5-100%) among the vaccinated survivors. Significant levels of IgM and IgG were induced among vaccinated animals, although they did not correlate with immunity. A mixed IgG1/IgG2 subclass profile was associated with the subunit vaccine, compared to the predominant IgG2 profile seen in bacterin vaccinated hamsters. These findings suggest that LigB(131

  7. Immunization with a recombinant bacillus Calmette-Guerin strain confers protective Th1 immunity against the human metapneumovirus.

    PubMed

    Palavecino, Christian E; Céspedes, Pablo F; Gómez, Roberto S; Kalergis, Alexis M; Bueno, Susan M

    2014-01-01

    Along with the human respiratory syncytial virus (hRSV), the human metapneumovirus (hMPV) is one of the leading causes of childhood hospitalization and a major health burden worldwide. Unfortunately, owing to an inefficient immunological memory, hMPV infection provides limited immune protection against reinfection. Furthermore, hMPV can induce an inadequate Th2 type immune response that causes severe lung inflammation, leading to airway obstruction. Similar to hRSV, it is likely that an effective clearance of hMPV would require a balanced Th1 type immunity by the host, involving the activation of IFN-γ-secreting T cells. A recognized inducer of Th1 immunity is Mycobacterium bovis bacillus Calmette-Guérin (BCG), which has been used in newborns for many decades and in several countries as a tuberculosis vaccine. We have previously shown that immunization with BCG strains expressing hRSV Ags can induce an efficient immune response that protects against this virus. In this study, we show that immunization with rBCG strains expressing the phosphoprotein from hMPV also can induce protective Th1 immunity. Mice immunized with rBCG were protected against weight loss, airway inflammation, and viral replication in the lungs after hMPV infection. Our rBCG vaccine also induced the activation of hMPV-specific T cells producing IFN-γ and IL-2, which could protect from hMPV infection when transferred to recipient mice. These data strongly support the notion that rBCG induces protective Th1 immunity and could be considered as an efficient vaccine against hMPV.

  8. The Challenge To Be Relevant in the 21st Century: Abstracts and Full Text Documents of Papers and Demos Given at the [International Association of Technological University Libraries] IATUL Conference (Pretoria, South Africa, June 1-5, 1998), Volume 18.

    ERIC Educational Resources Information Center

    International Association of Technological Univ. Libraries, Gothenburg (Sweden).

    This proceedings of the 1998 conference of the International Association of Technological University Libraries (IATUL) contains the full text of the following papers: "A Library Ready for 21st Century Services: The Case of the University of Science and Technology (UST) Library, Kumasi, Ghana" (Helena Rebecca Asamoah-Hassan);…

  9. International Conference of Directors of National Libraries on Resource Sharing in Asia and Oceanic [Proceedings] (Canberra, Australia, May 14-18, 1979). Development of Resource Sharing Networks. Networks Study No. 11.

    ERIC Educational Resources Information Center

    National Library of Australia, Canberra.

    The proceedings of this 1979 conference on library cooperation begin with proposals for the promotion of resource sharing among the national libraries of Asia and Oceania, the text of a policy statement on the role of national and international systems as approved at a 1976 meeting of directors of national libraries held in Lausanne, and a summary…

  10. Immune enhancement conferred by oral delivery of Lactobacillus rhamnosus HN001 in different milk-based substrates.

    PubMed

    Gill, H S; Rutherfurd, K J

    2001-11-01

    The probiotic Lactobacillus rhamnosus HN001 is known to enhance immunity in animal models, following oral delivery. In this study, we investigated the immunoenhancing effects of HN001 delivered to mice in different milk-based substrates, including: whole (full-fat) milk supplemented with HN001; fermented milk supplemented with HN001; or whole milk which had been part-fermented by HN001. Direct oral feeding of mice with HN001 in whole milk was shown to enhance the phagocytic activity of blood and peritoneal cells. Similar effects on phagocytosis were observed when UN001 was offered to mice in the form of a milk- or fermented milk-based diet. The degree of immune enhancement conferred by HN001 was similar whether the probiotic was used as an additive or as a fermentative agent. These studies confirm that Lb. rhamnosus HN001, derived originally from dairy food, enhances immune function following oral delivery in different milk bases.

  11. Brucella abortus ΔrpoE1 confers protective immunity against wild type challenge in a mouse model of brucellosis.

    PubMed

    Willett, Jonathan W; Herrou, Julien; Czyż, Daniel M; Cheng, Jason X; Crosson, Sean

    2016-09-30

    The Brucella abortus general stress response (GSR) system regulates activity of the alternative sigma factor, σ(E1), which controls transcription of approximately 100 genes and is required for persistence in a BALB/c mouse chronic infection model. We evaluated the host response to infection by a B. abortus strain lacking σ(E1) (ΔrpoE1), and identified pathological and immunological features that distinguish ΔrpoE1-infected mice from wild-type (WT), and that correspond with clearance of ΔrpoE1 from the host. ΔrpoE1 infection was indistinguishable from WT in terms of splenic bacterial burden, inflammation and histopathology up to 6weeks post-infection. However, Brucella-specific serum IgG levels in ΔrpoE1-infected mice were 5 times higher than WT by 4weeks post-infection, and remained significantly higher throughout the course of a 12-week infection. Total IgG and Brucella-specific IgG levels peaked strongly in ΔrpoE1-infected mice at 6weeks, which correlated with reduced splenomegaly and bacterial burden relative to WT-infected mice. Given the difference in immune response to infection with wild-type and ΔrpoE1, we tested whether ΔrpoE1 confers protective immunity to wild-type challenge. Mice immunized with ΔrpoE1 completely resisted WT infection and had significantly higher serum titers of Brucella-specific IgG, IgG2a and IFN-γ after WT challenge relative to age-matched naïve mice. We conclude that immunization of BALB/c mice with the B. abortus GSR pathway mutant, ΔrpoE1, elicits an adaptive immune response that confers significant protective immunity against WT infection.

  12. Wide screening of phage-displayed libraries identifies immune targets in planta.

    PubMed

    Rioja, Cristina; Van Wees, Saskia C; Charlton, Keith A; Pieterse, Corné M J; Lorenzo, Oscar; García-Sánchez, Susana

    2013-01-01

    Microbe-Associated Molecular Patterns and virulence effectors are recognized by plants as a first step to mount a defence response against potential pathogens. This recognition involves a large family of extracellular membrane receptors and other immune proteins located in different sub-cellular compartments. We have used phage-display technology to express and select for Arabidopsis proteins able to bind bacterial pathogens. To rapidly identify microbe-bound phage, we developed a monitoring method based on microarrays. This combined strategy allowed for a genome-wide screening of plant proteins involved in pathogen perception. Two phage libraries for high-throughput selection were constructed from cDNA of plants infected with Pseudomonas aeruginosa PA14, or from combined samples of the virulent isolate DC3000 of Pseudomonas syringae pv. tomato and its avirulent variant avrRpt2. These three pathosystems represent different degrees in the specificity of plant-microbe interactions. Libraries cover up to 2 × 10(7) different plant transcripts that can be displayed as functional proteins on the surface of T7 bacteriophage. A number of these were selected in a bio-panning assay for binding to Pseudomonas cells. Among the selected clones we isolated the ethylene response factor ATERF-1, which was able to bind the three bacterial strains in competition assays. ATERF-1 was rapidly exported from the nucleus upon infiltration of either alive or heat-killed Pseudomonas. Moreover, aterf-1 mutants exhibited enhanced susceptibility to infection. These findings suggest that ATERF-1 contains a microbe-recognition domain with a role in plant defence. To identify other putative pathogen-binding proteins on a genome-wide scale, the copy number of selected-vs.-total clones was compared by hybridizing phage cDNAs with Arabidopsis microarrays. Microarray analysis revealed a set of 472 candidates with significant fold change. Within this set defence-related genes, including well

  13. Wide Screening of Phage-Displayed Libraries Identifies Immune Targets in Planta

    PubMed Central

    Rioja, Cristina; Van Wees, Saskia C.; Charlton, Keith A.; Pieterse, Corné M. J.; Lorenzo, Oscar; García-Sánchez, Susana

    2013-01-01

    Microbe-Associated Molecular Patterns and virulence effectors are recognized by plants as a first step to mount a defence response against potential pathogens. This recognition involves a large family of extracellular membrane receptors and other immune proteins located in different sub-cellular compartments. We have used phage-display technology to express and select for Arabidopsis proteins able to bind bacterial pathogens. To rapidly identify microbe-bound phage, we developed a monitoring method based on microarrays. This combined strategy allowed for a genome-wide screening of plant proteins involved in pathogen perception. Two phage libraries for high-throughput selection were constructed from cDNA of plants infected with Pseudomonas aeruginosa PA14, or from combined samples of the virulent isolate DC3000 of Pseudomonas syringae pv. tomato and its avirulent variant avrRpt2. These three pathosystems represent different degrees in the specificity of plant-microbe interactions. Libraries cover up to 2×107 different plant transcripts that can be displayed as functional proteins on the surface of T7 bacteriophage. A number of these were selected in a bio-panning assay for binding to Pseudomonas cells. Among the selected clones we isolated the ethylene response factor ATERF-1, which was able to bind the three bacterial strains in competition assays. ATERF-1 was rapidly exported from the nucleus upon infiltration of either alive or heat-killed Pseudomonas. Moreover, aterf-1 mutants exhibited enhanced susceptibility to infection. These findings suggest that ATERF-1 contains a microbe-recognition domain with a role in plant defence. To identify other putative pathogen-binding proteins on a genome-wide scale, the copy number of selected-vs.-total clones was compared by hybridizing phage cDNAs with Arabidopsis microarrays. Microarray analysis revealed a set of 472 candidates with significant fold change. Within this set defence-related genes, including well

  14. International Federation of Library Associations Annual Conference Papers. Education and Research Division: Library Theory and Research Section (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Kolodziejska, Jadwiga; And Others

    Seven of these ten papers are concerned with library research in specific countries; the remaining three deal with library planning and ethics in research. Titles are "The Library as a Cultural Institution," by Jadwiga Kolodziejska, Poland; "The International Seminar 'Book and Library in Society' of the Polish Book and Readers…

  15. Induction of humoral immune response against Plasmodium falciparum sporozoites by immunization with a synthetic peptide mimotope whose sequence was derived from screening a filamentous phage epitope library.

    PubMed

    Stoute, J A; Ballou, W R; Kolodny, N; Deal, C D; Wirtz, R A; Lindler, L E

    1995-03-01

    The mouse monoclonal antibody 2A10 (immunoglobulin G), which recognizes the (NANP)n repeat of Plasmodium falciparum circumsporozoite surface protein, was used to screen a filamentous phage epitope library expressing random amino acid hexamers. The sequences obtained were TNRNPQ, SNRNPQ, NND-NPQ, SNYNPQ, and QNDNPQ (single-letter amino acid designation). These peptides showed 50% homology with the native epitope (PNANPN) and therefore were considered to mimic its structure (mimotopes). Two of these mimotopes (TNRNPQ and NNDNPQ) inhibited the binding of monoclonal antibody 2A10 to the recombinant protein R32LR, which contains the amino acid sequence [(NANP)15NVDP]2. Immunization of mice and rabbits using the peptide (TNRNPQ)4 induced a humoral response that recognized R32LR by an enzyme-linked immunosorbent assay and P. falciparum sporozoites by an immunofluorescence assay. These results suggest that phage epitope libraries can be exploited to screen for mimotopes in the design of subunit vaccines against infectious agents.

  16. Nasal Immunization Confers High Avidity Neutralizing Antibody Response and Immunity to Primary and Recurrent Genital Herpes in Guinea Pigs.

    PubMed

    Persson, Josefine; Zhang, Yuan; Olafsdottir, Thorunn A; Thörn, Karolina; Cairns, Tina M; Wegmann, Frank; Sattentau, Quentin J; Eisenberg, Roselyn J; Cohen, Gary H; Harandi, Ali M

    2016-01-01

    Genital herpes is one of the most prevalent sexually transmitted infections in both the developing and developed world. Following infection, individuals experience life-long latency associated with sporadic ulcerative outbreaks. Despite many efforts, no vaccine has yet been licensed for human use. Herein, we demonstrated that nasal immunization with an adjuvanted HSV-2 gD envelope protein mounts significant protection to primary infection as well as the establishment of latency and recurrent genital herpes in guinea pigs. Nasal immunization was shown to elicit specific T cell proliferative and IFN-γ responses as well as systemic and vaginal gD-specific IgG antibody (Ab) responses. Furthermore, systemic IgG Abs displayed potent HSV-2 neutralizing properties and high avidity. By employing a competitive surface plasmon resonance (SPR) analysis combined with a battery of known gD-specific neutralizing monoclonal Abs (MAbs), we showed that nasal immunization generated IgG Abs directed to two major discontinuous neutralizing epitopes of gD. These results highlight the potential of nasal immunization with an adjuvanted HSV-2 envelope protein for induction of protective immunity to primary and recurrent genital herpes.

  17. Nasal Immunization Confers High Avidity Neutralizing Antibody Response and Immunity to Primary and Recurrent Genital Herpes in Guinea Pigs

    PubMed Central

    Persson, Josefine; Zhang, Yuan; Olafsdottir, Thorunn A.; Thörn, Karolina; Cairns, Tina M.; Wegmann, Frank; Sattentau, Quentin J.; Eisenberg, Roselyn J.; Cohen, Gary H.; Harandi, Ali M.

    2016-01-01

    Genital herpes is one of the most prevalent sexually transmitted infections in both the developing and developed world. Following infection, individuals experience life-long latency associated with sporadic ulcerative outbreaks. Despite many efforts, no vaccine has yet been licensed for human use. Herein, we demonstrated that nasal immunization with an adjuvanted HSV-2 gD envelope protein mounts significant protection to primary infection as well as the establishment of latency and recurrent genital herpes in guinea pigs. Nasal immunization was shown to elicit specific T cell proliferative and IFN-γ responses as well as systemic and vaginal gD-specific IgG antibody (Ab) responses. Furthermore, systemic IgG Abs displayed potent HSV-2 neutralizing properties and high avidity. By employing a competitive surface plasmon resonance (SPR) analysis combined with a battery of known gD-specific neutralizing monoclonal Abs (MAbs), we showed that nasal immunization generated IgG Abs directed to two major discontinuous neutralizing epitopes of gD. These results highlight the potential of nasal immunization with an adjuvanted HSV-2 envelope protein for induction of protective immunity to primary and recurrent genital herpes. PMID:28082979

  18. Virus-like nanoparticle and DNA vaccination confers protection against respiratory syncytial virus by modulating innate and adaptive immune cells

    PubMed Central

    Ko, Eun-Ju; Kwon, Young-Man; Lee, Jong Seok; Hwang, Hye Suk; Yoo, Si-Eun; Lee, Yu-Na; Lee, Young-Tae; Kim, Min-Chul; Cho, Min Kyoung; Lee, You Ri; Quan, Fu-Shi; Song, Jae-Min; Lee, Sujin; Moore, Martin L.; Kang, Sang-Moo

    2014-01-01

    Respiratory syncytial virus (RSV) is an important human pathogen. Expression of virus structural proteins produces self-assembled virus-like nanoparticles (VLP). We investigated immune phenotypes after RSV challenge of immunized mice with VLP containing RSV F and G glycoproteins mixed with F-DNA (FdFG VLP). In contrast to formalin-inactivated RSV (FIRSV) causing vaccination-associated eosinophilia, FdFG VLP immunization induced low bronchoalveolar cellularity, higher ratios of CD11c+ versus CD11b+ phenotypic cells and CD8+ T versus CD4+ T cells secreting interferon (IFN)-γ, T helper type-1 immune responses, and no sign of eosinophilia upon RSV challenge. Furthermore, RSV neutralizing activity, lung viral clearance, and histology results suggest that FdFG VLP can be comparable to live RSV in conferring protection against RSV and in preventing RSV disease. This study provides evidence that a combination of recombinant RSV VLP and plasmid DNA may have a potential anti-RSV prophylactic vaccine inducing balanced innate and adaptive immune responses. PMID:25109662

  19. Thymus cDNA library survey uncovers novel features of immune molecules in Chinese giant salamander Andrias davidianus.

    PubMed

    Zhu, Rong; Chen, Zhong-Yuan; Wang, Jun; Yuan, Jiang-Di; Liao, Xiang-Yong; Gui, Jian-Fang; Zhang, Qi-Ya

    2014-10-01

    A ranavirus-induced thymus cDNA library was constructed from Chinese giant salamander, the largest extant amphibian species. Among the 137 putative immune-related genes derived from this library, these molecules received particular focus: immunoglobulin heavy chains (IgM, IgD, and IgY), IFN-inducible protein 6 (IFI6), and T cell receptor beta chain (TCRβ). Several unusual features were uncovered: IgD displays a structure pattern distinct from those described for other amphibians by having only four constant domains plus a hinge region. A unique IgY form (IgY(ΔFc)), previously undescribed in amphibians, is present in serum. Alternative splicing is observed to generate IgH diversification. IFI6 is newly-identified in amphibians, which occurs in two forms divergent in subcelluar distribution and antiviral activity. TCRβ immunoscope profile follows the typical vertebrate pattern, implying a polyclonal T cell repertoire. Collectively, the pioneering survey of ranavirus-induced thymus cDNA library from Chinese giant salamander reveals immune components and characteristics in this primitive amphibian.

  20. Chloroplast-derived vaccine antigens confer dual immunity against cholera and malaria by oral or injectable delivery

    PubMed Central

    Davoodi-Semiromi, Abdoreza; Schreiber, Melissa; Nallapali, Samson; Verma, Dheeraj; Singh, Nameirakpam D.; Banks, Robert K.; Chakrabarti, Debopam; Daniell, Henry

    2009-01-01

    Summary Cholera and malaria are major diseases causing high mortality. The only licensed cholera vaccine is expensive; immunity is lost in children within 3 years and adults are not fully protected. No vaccine is yet available for malaria. Therefore, in this study, the cholera toxin-B subunit (CTB) of Vibrio cholerae fused to malarial vaccine antigens apical membrane antigen-1 (AMA1) and merozoite surface protein-1 (MSP1) was expressed in lettuce and tobacco chloroplasts. Southern blot analysis confirmed homoplasmy and stable integration of transgenes. CTB-AMA1 and CTB-MSP1 fusion proteins accumulated up to 13.17% and 10.11% (total soluble protein, TSP) in tobacco and up to 7.3% and 6.1% (TSP) in lettuce respectively. Nine groups of mice (n = 10/group) were immunized subcutaneously (SQV) or orally (ORV) with purified antigens or transplastomic tobacco leaves. Significant levels of antigen-specific antibody titres of immunized mice completely inhibited proliferation of the malarial parasite and cross-reacted with the native parasite proteins in immunoblots and immunofluorescence studies. Protection against cholera toxin challenge in both ORV (100%) and SQV (89%) mice correlated with CTB-specific titres of intestinal, serum IgA and IgG1 in ORV and only IgG1 in SQV mice, but no other immunoglobulin. Increasing numbers of interleukin-10+ T cell but not Foxp3+ regulatory T cells, suppression of interferon-γ and absence of interleukin-17 were observed in protected mice, suggesting that immunity is conferred via the Tr1/Th2 immune response. Dual immunity against two major infectious diseases provided by chloroplast-derived vaccine antigens for long-term (>300 days, 50% of mouse life span) offers a realistic platform for low cost vaccines and insight into mucosal and systemic immunity. PMID:20051036

  1. Induction of anti-EGFR immune response with mimotopes identified from a phage display peptide library by panitumumab

    PubMed Central

    Qu, Hong; Di, Jiabo; Wang, Zaozao; Xing, Jiadi; Wu, Fan; Wu, Wei; Wang, Xicheng; Shen, Lin; Jiang, Beihai; Su, Xiangqian

    2016-01-01

    The epidermal growth factor receptor (EGFR) is overexpressed in several epithelial tumors. Anti-EGFR humanized monoclonal antibodies, cetuximab and panitumumab, in combination with chemotherapy have improved the prognosis for patients with wild-type RAS tumors. To identify mimotopes of EGFR and develop mimotope-based EGFR vaccines, we screened a phage display peptide library with panitumumab. Two EGFR mimotopes P19 and P26, which could be recognized by panitumumab specifically, were isolated. To enhance the immune responses, we generated recombinant proteins of P19 or P26 fused to a heat-shock cognate protein 70 (Hsc70), and evaluated the efficacy of Hsc70-P19 and Hsc70-P26 as vaccines in vivo. Immunization with Hsc70-P19 or Hsc70-P26 fusion protein stimulated the immune system to produce specific antibodies against peptides as well as EGFR. Moreover, antibodies elicited against mimotopes could induce antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and inhibit the proliferation of EGFR-overexpressing A431 cells. Treatment with Hsc70-P19 and Hsc70-P26 significantly reduced tumor growth in BALB/c transplantable lung cancer models. Although there was no sequence homology between the phage-derived peptides and EGFR by alignments, both peptides mimic the conformational structure of EGFR binding to panitumumab. In conclusion, the mimotopes we identified from phage display peptide library could be promising candidate vaccines for active anti-EGFR immunotherapy against cancers. PMID:27659529

  2. Induction of anti-EGFR immune response with mimotopes identified from a phage display peptide library by panitumumab.

    PubMed

    Wang, Aidong; Cui, Ming; Qu, Hong; Di, Jiabo; Wang, Zaozao; Xing, Jiadi; Wu, Fan; Wu, Wei; Wang, Xicheng; Shen, Lin; Jiang, Beihai; Su, Xiangqian

    2016-11-15

    The epidermal growth factor receptor (EGFR) is overexpressed in several epithelial tumors. Anti-EGFR humanized monoclonal antibodies, cetuximab and panitumumab, in combination with chemotherapy have improved the prognosis for patients with wild-type RAS tumors. To identify mimotopes of EGFR and develop mimotope-based EGFR vaccines, we screened a phage display peptide library with panitumumab. Two EGFR mimotopes P19 and P26, which could be recognized by panitumumab specifically, were isolated. To enhance the immune responses, we generated recombinant proteins of P19 or P26 fused to a heat-shock cognate protein 70 (Hsc70), and evaluated the efficacy of Hsc70-P19 and Hsc70-P26 as vaccines in vivo. Immunization with Hsc70-P19 or Hsc70-P26 fusion protein stimulated the immune system to produce specific antibodies against peptides as well as EGFR. Moreover, antibodies elicited against mimotopes could induce antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and inhibit the proliferation of EGFR-overexpressing A431 cells. Treatment with Hsc70-P19 and Hsc70-P26 significantly reduced tumor growth in BALB/c transplantable lung cancer models. Although there was no sequence homology between the phage-derived peptides and EGFR by alignments, both peptides mimic the conformational structure of EGFR binding to panitumumab. In conclusion, the mimotopes we identified from phage display peptide library could be promising candidate vaccines for active anti-EGFR immunotherapy against cancers.

  3. Rethinking the Library in the Information Age: What Role for Research? [Summary of a Conference/Workshop on Building a Library Research Infrastructure (October 31-November 1, 1988)].

    ERIC Educational Resources Information Center

    Holley, Edward G.; Kenan, William Rand, Jr.

    The question "What is the role for research in library and information science?" lies at the heart of an ongoing project sponsored by the Office of Library Programs in the U.S. Department of Education. The project, which was begun in 1986-87, is designed to identify researchable issues that could help libraries attain or maintain a leadership…

  4. Intranasal immunization with inactivated feline calicivirus particles confers robust protection against homologous virus and suppression against heterologous virus in cats.

    PubMed

    Sato, Hiroaki; Sehata, Go; Okada, Nobutaka; Iwamoto, Kayo; Masubuchi, Katsuo; Kainuma, Risa; Noda, Tatsuki; Igarashi, Tatsuhiko; Sawada, Takuo; Noro, Taichi; Oishi, Eiji

    2017-07-01

    The protective efficacy of intranasal (IN) administration of inactivated feline calicivirus (FCV) vaccine against homologous or heterologous FCV infection was investigated. Groups of cats immunized with the experimental inactivated, non-adjuvanted FCV vaccine via either the IN or subcutaneous (SC) route were exposed to homologous or highly heterologous FCV. Both the IN and SC immunization protocols established robust protection against homologous FCV infection. Although neither immunization regimen conferred protection against the heterologous strain, clinical scores and virus titres of oral swabs were lower in cats in the IN group compared to those in the SC group, accompanying a faster neutralizing antibody response against the heterologous virus in cats in the IN group. The IN group secreted more IgA specific to FCV proteins in oral washes (lavage fluids from the oral cavity) than the SC group. IN immunization with an inactivated whole FCV particle, which protects cats from homologous virus exposure and shortens the period of heterologous virus shedding, may serve as a better platform for anti-FCV vaccine.

  5. Coadministration of protoxin Cry1Ac from Bacillus thuringiensis with metacestode extract confers protective immunity to murine cysticercosis.

    PubMed

    Ibarra-Moreno, S; García-Hernández, A L; Moreno-Fierros, L

    2014-06-01

    The Bacillus thuringiensis Cry1Ac protoxin (pCry1Ac) is a promising mucosal immunogen and adjuvant that induces protective immunity against Naegleria fowleri and malaria infection models. We determined whether pCry1Ac acted as a protective adjuvant against infection with Taenia crassiceps. BALB/C mice were thrice i.p. immunized with (i) pCry1Ac, (ii) metacestode extract, (iii) extract + pCry1Ac or (iv) vehicle, challenged with metacestodes on day 26 and then sacrificed 35 days later. Cysticerci in the peritoneal cavity were counted, while the serum antibody response and cytokines were analysed after immunization and during infection. Only immunization with pCry1Ac plus extract conferred a significant protection (up to 47%). This group presented fluctuating antibody peaks during infection and the highest IgG1 and IgM titres. Immunization with extract alone elicited high IgG1 and the highest IgG2a responses after 25 days of infection, while nonimmunized mice presented a poor, mixed-Th1/Th2 response during infection. Sharp peaks of TNFα and IFN-γ occurred immediately after the first immunization with extract, especially in the presence of pCry1Ac, but not after the challenge, while in the control and pCry1Ac-alone groups, cytokines were only detected after the challenge. The data support the protective-adjuvant effect of co-administration of pCry1Ac in cysticercosis.

  6. IFLA General Conference, 1987. Division of Management and Technology. Library Building and Equipment Section. Information Technology Section. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The six papers in this collection focus on library buildings and equipment and information technology: (1) "Library Equipment in a Changing Library Environment" (Margaret Beckman, Canada); (2) "The New Building for the British Library" (Colin St. John Wilson, United Kingdom); (3) "User Interaction with Online Catalogue" (Stephen Walker, United…

  7. Genetic diversity confers colony-level benefits due to individual immunity

    USDA-ARS?s Scientific Manuscript database

    Several costs and benefits arise as a consequence of eusociality and group-living. With increasing group size, spread of disease among nest-mates poses selective pressure on both individual immunity and group-level mechanisms of disease resistance (social immunity). Another factor known to influence...

  8. Genetic diversity confers colony-level benefits due to individual immunity.

    PubMed

    Simone-Finstrom, Michael; Walz, Megan; Tarpy, David R

    2016-03-01

    Several costs and benefits arise as a consequence of eusociality and group-living. With increasing group size, spread of disease among nest-mates poses selective pressure on both individual immunity and group-level mechanisms of disease resistance (social immunity). Another factor known to influence colony-level expression of disease is intracolony genetic diversity, which in honeybees (Apis mellifera) is a direct function of the number of mates of the queen. Colonies headed by queens with higher mating numbers have less variable infections of decreased intensity, though the underlying mechanisms remain unclear. By pathogen-challenging larvae in vitro, we decoupled larval immune response from mechanisms of social immunity. Our results show that baseline immunity and degree of immune response do not vary with genetic diversity. However, intracolony variance in antimicrobial peptide production after pathogen challenge decreases with increasing genetic diversity. This reduction in variability of the larval immune response could drive the mitigation of disease observed in genetically diverse colonies. © 2016 The Author(s).

  9. Genetic diversity confers colony-level benefits due to individual immunity

    PubMed Central

    Walz, Megan; Tarpy, David R.

    2016-01-01

    Several costs and benefits arise as a consequence of eusociality and group-living. With increasing group size, spread of disease among nest-mates poses selective pressure on both individual immunity and group-level mechanisms of disease resistance (social immunity). Another factor known to influence colony-level expression of disease is intracolony genetic diversity, which in honeybees (Apis mellifera) is a direct function of the number of mates of the queen. Colonies headed by queens with higher mating numbers have less variable infections of decreased intensity, though the underlying mechanisms remain unclear. By pathogen-challenging larvae in vitro, we decoupled larval immune response from mechanisms of social immunity. Our results show that baseline immunity and degree of immune response do not vary with genetic diversity. However, intracolony variance in antimicrobial peptide production after pathogen challenge decreases with increasing genetic diversity. This reduction in variability of the larval immune response could drive the mitigation of disease observed in genetically diverse colonies. PMID:26961896

  10. 99th Dahlem Conference on Infection, Inflammation and Chronic Inflammatory Disorders: Innate immune responses in plants

    PubMed Central

    Schulze-Lefert, P

    2010-01-01

    Plants rely exclusively upon mechanisms of innate immunity. Current concepts of the plant innate immune system are based largely on two forms of immunity that engage distinct classes of immune receptors. These receptors enable the recognition of non-self structures that are either conserved between members of a microbial class or specific to individual strains of a microbe. One type of receptor comprises membrane-resident pattern recognition receptors (PRRs) that detect widely conserved microbe-associated molecular patterns (MAMPs) on the cell surface. A second type of mainly intracellular immune sensors, designated resistance (R) proteins, recognizes either the structure or function of strain-specific pathogen effectors that are delivered inside host cells. Phytopathogenic microorganisms have evolved a repertoire of effectors, some of which are delivered into plant cells to sabotage MAMP-triggered immune responses. Plants appear to have also evolved receptors that sense cellular injury by the release and perception of endogenous damage-associated molecular patterns (DAMPs). It is possible that the integration of MAMP and DAMP responses is critical to mount robust MAMP-triggered immunity. This signal integration might help to explain why plants are colonized in nature by remarkably diverse and seemingly asymptomatic microbial communities. PMID:20415853

  11. Selection of bisphenol A - single-chain antibodies from a non-immunized mouse library by ribosome display.

    PubMed

    Zhao, Li; Ning, Baoan; Bai, Jialei; Chen, Xiang; Peng, Yuan; Sun, Siming; Li, Guimin; Fan, Xianjun; Liu, Yuanyuan; Liu, Jianqing; Sun, Yanan; Gao, Zhixian; Zhang, Juankun

    2015-11-01

    Developing reagents with high affinity and specificity are critical to detect the environmental hormones or toxicants. Ribosome display technology has been widely used in functional protein or peptide screening and in directed evolution of protein molecules in vitro. In this study, single-chain variable fragments (scFvs) against bisphenol A (BPA) were selected from a library constructed from splenocytes of non-immunized mice. After five rounds of selection, the selected scFvs bound to BPA with high affinity. Indirect competitive enzyme-linked immunosorbent assay (ELISA) was introduced to screen the antibody affinity and specificity to BPA. The equilibrium dissociation constants (KDS) of one clone was 1.76μM as determined by surface plasmon resonance (SPR). This study indicated that ribosome display can isolate binders to small molecules from a non-immunized naive library without any in vivo steps and can generate recombinant antibodies efficiently and rapidly. In addition, this study provides a methodological framework for detection of small molecules using recombinant antibodies.

  12. Development, screening, and analysis of DNA aptamer libraries potentially useful for diagnosis and passive immunity of arboviruses

    PubMed Central

    2012-01-01

    Background Nucleic acid aptamers have long demonstrated the capacity to bind viral envelope proteins and to inhibit the progression of pathogenic virus infections. Here we report on initial efforts to develop and screen DNA aptamers against recombinant envelope proteins or synthetic peptides and whole inactivated viruses from several virulent arboviruses including Chikungunya, Crimean-Congo hemorrhagic fever (CCHF), dengue, tickborne encephalitis and West Nile viruses. We also analyzed sequence data and secondary structures for commonalities that might reveal consensus binding sites among the various aptamers. Some of the highest affinity and most specific aptamers in the down-selected libraries were demonstrated to have diagnostic utility in lateral flow chromatographic assays and in a fluorescent aptamer-magnetic bead sandwich assay. Some of the reported aptamers may also be able to bind viral envelope proteins in vivo and therefore may have antiviral potential in passive immunity or prophylactic applications. Results Several arbovirus DNA aptamer sequences emerged multiple times in the various down selected aptamer libraries thereby suggesting some consensus sequences for binding arbovirus envelope proteins. Screening of aptamers by enzyme-linked aptamer sorbent assay (ELASA) was useful for ranking relative aptamer affinities against their cognate viral targets. Additional study of the aptamer sequences and secondary structures of top-ranked anti-arboviral aptamers suggest potential virus binding motifs exist within some of the key aptamers and are highlighted in the supplemental figures for this article. One sequence segment (ACGGGTCCGGACA) emerged 60 times in the anti-CCHF aptamer library, but nowhere else in the anti-arbovirus library and only a few other times in a larger library of aptamers known to bind bacteria and rickettsia or other targets. Diagnostic utility of some of the aptamers for arbovirus detection in lateral flow chromatographic assays and a

  13. IFLA General Conference, 1991. Division of Serving the General Public: Section of Public Libraries; Section of Children's Libraries; Section on School Libraries; Section of Libraries Serving Disadvantaged Persons; Section of Libraries for the Blind; Section of Library Services to Multicultural Populations; ROTNAC (RT); Mobile Libraries (RT). Booklet 3.

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    The 17 papers in this booklet were presented at meetings of 7 sections within the Division of Serving the General Public: (1) "Overcoming Illiteracy and Public Library Social Functions Development" (Slava Matlina, USSR); (2) "Impact of Economic and Political Change upon Public Library Services" (E. S. Ponomaryova, USSR); (3)…

  14. Year of the Oceans: Science of Information Handling. [Proceedings of the] Annual Conference of the International Association of Marine Science Libraries and Information Centers (10th, Woods Hole, Massachusetts, October 2-5, 1984).

    ERIC Educational Resources Information Center

    Grundy, R. L., Ed.; Ford, R. T., Ed.

    International Association of Marine Science Libraries and Information Centers (IAMSLIC) conferences provide a format for libraries and information specialists to discuss common interests and concerns so that services and information can be made available to scientists, administrators, engineers, educators and students in the discipline of marine…

  15. International Federation of Library Associations Annual Conference Papers. Collections and Services Division: Interlending, Rare and Precious Books, and Exchange and Acquisition Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Line, Maurice B.; And Others

    This set of papers presented to the Collections and Services Division of the International Federation of Library Associations at its 47th annual conference (1981) includes: "Planning Interlending Systems in Developing Countries," by Maurice B. Line; "Problems of Centralisation of Inter-Library Lending in a De-Centralized Library…

  16. The School Library: Centre for Life-Long Learning. Proceedings of the Annual Conference of the International Association of School Librarianship (18th, Subang Jaya, Selangor, Malaysia, July 22-26, 1989).

    ERIC Educational Resources Information Center

    Siong, Wong Kim, Ed.; And Others

    This conference report contains 5 addresses and 29 reports on the general theme of the school library as a center for lifelong learning. Submitted by librarians throughout the world, the reports reflect theoretical as well as practical perspectives, and include the following topics: (1) establishing, developing, and evaluating library resource…

  17. International Federation of Library Associations Annual Conference Papers. Collections and Services Division: Interlending, Rare and Precious Books, and Exchange and Acquisition Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Line, Maurice B.; And Others

    This set of papers presented to the Collections and Services Division of the International Federation of Library Associations at its 47th annual conference (1981) includes: "Planning Interlending Systems in Developing Countries," by Maurice B. Line; "Problems of Centralisation of Inter-Library Lending in a De-Centralized Library…

  18. Year of the Oceans: Science of Information Handling. [Proceedings of the] Annual Conference of the International Association of Marine Science Libraries and Information Centers (10th, Woods Hole, Massachusetts, October 2-5, 1984).

    ERIC Educational Resources Information Center

    Grundy, R. L., Ed.; Ford, R. T., Ed.

    International Association of Marine Science Libraries and Information Centers (IAMSLIC) conferences provide a format for libraries and information specialists to discuss common interests and concerns so that services and information can be made available to scientists, administrators, engineers, educators and students in the discipline of marine…

  19. Immunization with BLS-Stx2B chimera totally protects dams from early pregnancy loss induced by Shiga toxin type 2 (Stx2) and confers anti-Stx2 immunity to the offspring.

    PubMed

    Sacerdoti, Flavia; Mejías, María P; Bruballa, Andrea C; Alvarez, Romina Soledad; Amaral, María M; Palermo, Marina S; Ibarra, Cristina

    2016-09-07

    Shiga toxin producing Escherichia coli (STEC) are bacterial pathogens involved in food-borne diseases. Shiga toxin (Stx) is the main virulence factor of STEC and is responsible for systemic complications including Hemolytic Uremic Syndrome (HUS). It has been previously demonstrated that Shiga toxin type 2 (Stx2) induces pregnancy loss in rats in early stage of pregnancy. The main purpose of this study was to determine if an active immunization prevents Stx2 mediated pregnancy loss and confers passive protective immunity to the offspring. For that purpose Sprague Dawley female rats were immunized with the chimera based on the enzyme lumazine synthase from Brucella spp. (BLS) and the B subunit of Shiga toxin 2 (Stx2B) named BLS-Stx2B. After immunization females were mated with males. At day 8 of gestation, dams were challenged intraperitoneally with a sublethal and abortifacient dose of Stx2. The immunization induced high anti-Stx2B-specific antibody titers in sera and most important, prevented pregnancy loss. Pups born and breastfeed by immunized dams had high anti-Stx2B-specific antibody titers in sera. Cross-fostering experiments indicated that passive protective immunity against Stx2 was transmitted through lactation. These results indicate that immunization of adult female rats with BLS-Stx2B prevents Stx2-induced pregnancy loss and confers anti Stx2 protective immunity to the offspring. Copyright © 2016 Elsevier Ltd. All rights reserved.

  20. Utilization of Multi-Immunization and Multiple Selection Strategies for Isolation of Hapten-Specific Antibodies from Recombinant Antibody Phage Display Libraries

    PubMed Central

    Tullila, Antti; Nevanen, Tarja K.

    2017-01-01

    Phage display technology provides a powerful tool for the development of novel recombinant antibodies. In this work, we optimized and streamlined the recombinant antibody discovery process for haptens as an example. A multi-immunization approach was used in order to avoid the need for construction of multiple antibody libraries. Selection methods were developed to utilize the full potential of the recombinant antibody library by applying four different elution conditions simultaneously. High-throughput immunoassays were used to analyse the binding properties of the individual antibody clones. Different carrier proteins were used in the immunization, selection, and screening phases to avoid enrichment of the antibodies for the carrier protein epitopes. Novel recombinant antibodies against mycophenolic acid and ochratoxin A, with affinities up to 39 nM and 34 nM, respectively, were isolated from a multi-immunized fragment antigen-binding (Fab) library. PMID:28561803

  1. Utilization of Multi-Immunization and Multiple Selection Strategies for Isolation of Hapten-Specific Antibodies from Recombinant Antibody Phage Display Libraries.

    PubMed

    Tullila, Antti; Nevanen, Tarja K

    2017-05-31

    Phage display technology provides a powerful tool for the development of novel recombinant antibodies. In this work, we optimized and streamlined the recombinant antibody discovery process for haptens as an example. A multi-immunization approach was used in order to avoid the need for construction of multiple antibody libraries. Selection methods were developed to utilize the full potential of the recombinant antibody library by applying four different elution conditions simultaneously. High-throughput immunoassays were used to analyse the binding properties of the individual antibody clones. Different carrier proteins were used in the immunization, selection, and screening phases to avoid enrichment of the antibodies for the carrier protein epitopes. Novel recombinant antibodies against mycophenolic acid and ochratoxin A, with affinities up to 39 nM and 34 nM, respectively, were isolated from a multi-immunized fragment antigen-binding (Fab) library.

  2. Art Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on art libraries, librarianship, and documentation presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "The Tyranny of Distance: Art Libraries in Canada," a description by Mary F. Williamson of Canada's regional art libraries which serve both art students and the general public;…

  3. A Peptide Domain on Gingipain R Which Confers Immunity against Porphyromonas gingivalis Infection in Mice

    PubMed Central

    Genco, Caroline Attardo; Odusanya, Basil Michael; Potempa, Jan; Mikolajczyk-Pawlinska, Jowita; Travis, James

    1998-01-01

    The cysteine proteinases referred to as gingipains R (gingipain R1 and gingipain R2) and gingipain K produced by Porphyromonas gingivalis are virulence factors of this periodontal pathogen which likely act by interrupting host defense mechanisms and by participating in the penetration and destruction of host connective tissue. To examine the effect of immunization with gingipains R on the ability of P. gingivalis to colonize and invade in the mouse chamber model, BALB/c mice were immunized intraperitoneally with the 95-kDa gingipain R1, the 50-kDa gingipain R2, or multiple antigenic peptide (MAP)-conjugated gingipain R-derived peptides and then challenged with P. gingivalis. Immunization of mice with the 95-kDa gingipain R1, the 50-kDa gingipain R2, or a peptide derived from the N-terminal sequence of the catalytic domain of gingipains R (peptide A) followed by challenge with P. gingivalis A7436 resulted in protection from P. gingivalis invasion. In contrast, immunization with peptides corresponding to either a sequence encompassing the catalytic cysteine residue of gingipains R (peptide B) or an identical sequence within the catalytic domains of gingipain R1 and gingipain K (peptide C), followed by challenge with P. gingivalis, did not protect animals, nor did immunization with a peptide corresponding to sequences within the adhesion/hemagglutinin domain of gingipain R1 (peptide D) which have been shown to be directly involved in the hemagglutinin activity of gingipain R1. However, the immunoglobulin G (IgG) titer obtained following immunization with peptide D was comparable to that obtained following immunization with the N-terminal peptide (peptide A). Competitive enzyme-linked immunosorbent assays, using either the 95-kDa gingipain R1 or gingipain K as the competing soluble antigen, indicated that 42 and 53% of the antibodies induced by immunization with heat-killed bacteria recognize gingipain R1 and gingipain K, respectively; however, even at very high

  4. State Library Conferences as Professional Development Venues: Unbalanced Support for the AASL-Defined Roles of the School Librarian

    ERIC Educational Resources Information Center

    Moreillon, Judi; Cahill, Maria; McKee, Rebecca

    2012-01-01

    The American Association of School Librarians (AASL) released new guidelines for school library programs in the summer of 2009. Empowering Learners: Guidelines for School Library Programs (AASL 2009a), hereafter referred to as EL, spells out the five roles that school librarians must practice to empower library users. The purpose of this…

  5. International Federation of Library Associations Annual Conference Papers. Education and Research Division: Library Schools and Other Training Aspects, and Round Table on Library History Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Wagenbreth, Hildegard; And Others

    This group of six papers centers on the development of library schools and the training of library personnel. "The Status of Professional Groups in Libraries and Library Education in the GDR," by Hildegard Wagenbreth and Helmut Kubitschek, East Germany, describes the training programs, apprenticeships, courses, and admission criteria of…

  6. International Federation of Library Associations Annual Conference Papers. Education and Research Division: Library Schools and Other Training Aspects, and Round Table on Library History Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Wagenbreth, Hildegard; And Others

    This group of six papers centers on the development of library schools and the training of library personnel. "The Status of Professional Groups in Libraries and Library Education in the GDR," by Hildegard Wagenbreth and Helmut Kubitschek, East Germany, describes the training programs, apprenticeships, courses, and admission criteria of…

  7. Defining the role of the immune system in cancer treatment: highlights from the Immunochemotherapy Conference.

    PubMed

    Demaria, Sandra

    2011-06-01

    The Immunochemotherapy: Correcting Immune Escape in Cancer meeting was co-organized by George Prendergast (Lankenau Institute for Medical Research, PA, USA), Guido Kroemer (Gustave Roussy Cancer Institute, Paris, France) and Laurence Zitvogel (Gustave Roussy Cancer Institute) in partnership with Abcam. It brought together investigators with a diverse background in immunology, oncology and cancer biology, and offered a forum for discussion of a new vision that acknowledges the role of the host immune system not only in cancer development and progression, but also as a major determinant of response to treatment. An important implication of this vision is that synergy between conventional cytocidal modalities and immune response modifiers is not only possible, but holds the promise to revolutionize cancer treatment. The emerging evidence that was presented in support of this new concept will be summarized in this article.

  8. Vaccination with Lentiviral Vector Expressing the nfa1 Gene Confers a Protective Immune Response to Mice Infected with Naegleria fowleri

    PubMed Central

    Kim, Jong-Hyun; Sohn, Hae-Jin; Lee, Jinyoung; Yang, Hee-Jong; Chwae, Yong-Joon; Kim, Kyongmin; Park, Sun

    2013-01-01

    Naegleria fowleri, a pathogenic free-living amoeba, causes fatal primary amoebic meningoencephalitis (PAM) in humans and animals. The nfa1 gene (360 bp), cloned from a cDNA library of N. fowleri, produces a 13.1-kDa recombinant protein which is located on pseudopodia, particularly the food cup structure. The nfa1 gene plays an important role in the pathogenesis of N. fowleri infection. To examine the effect of nfa1 DNA vaccination against N. fowleri infection, we constructed a lentiviral vector (pCDH) expressing the nfa1 gene. For the in vivo mouse study, BALB/c mice were intranasally vaccinated with viral particles of a viral vector expressing the nfa1 gene. To evaluate the effect of vaccination and immune responses of mice, we analyzed the IgG levels (IgG, IgG1, and IgG2a), cytokine induction (interleukin-4 [IL-4] and gamma interferon [IFN-γ]), and survival rates of mice that developed PAM. The levels of both IgG and IgG subclasses (IgG1 and IgG2a) in vaccinated mice were significantly increased. The cytokine analysis showed that vaccinated mice exhibited greater IL-4 and IFN-γ production than the other control groups, suggesting a Th1/Th2 mixed-type immune response. In vaccinated mice, high levels of Nfa1-specific IgG antibodies continued until 12 weeks postvaccination. The mice vaccinated with viral vector expressing the nfa1 gene also exhibited significantly higher survival rates (90%) after challenge with N. fowleri trophozoites. Finally, the nfa1 vaccination effectively induced protective immunity by humoral and cellular immune responses in N. fowleri-infected mice. These results suggest that DNA vaccination using a viral vector may be a potential tool against N. fowleri infection. PMID:23677321

  9. Libraries, Archives, and Museums Helping Create Futures: Building on Culture, Knowledge, and Information through Collaboration and Resource Sharing. Selected Papers from PIALA 2010, Pacific Islands Association of Libraries, Archives, and Museums Annual Conference (20th, Weno, Chuuk State, Federated States of Micronesia, November 15-19, 2010)

    ERIC Educational Resources Information Center

    Drake, Paul B., Ed.

    2012-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 20th annual conference was held in Weno, Chuuk State, Federated States of Micronesia, November 15-19, 2010. The volume begins with a listing of the members of the PIALA 2010…

  10. It Can Be Done: With Advocacy, Libraries Can Thrive on Pacific Islands. Selected Papers from PIALA 2007, Pacific Islands Association of Libraries, Archives and Museums Annual Conference (17th, Tinian and Saipan, Commonwealth of the Northern Mariana Islands, November 12-17, 2007)

    ERIC Educational Resources Information Center

    Horie, Ruth H., Ed.

    2010-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. The volume begins with a listing of the members of the PIALA 2007 Planning Committee, the Officers and Executive Board, and Acknowledgements, followed by the Pre-Conference Workshop…

  11. New insight in quantitative analysis of vascular permeability during immune reaction (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Kalchenko, Vyacheslav; Molodij, Guillaume; Kuznetsov, Yuri; Smolyakov, Yuri; Israeli, David; Meglinski, Igor; Harmelin, Alon

    2016-03-01

    The use of fluorescence imaging of vascular permeability becomes a golden standard for assessing the inflammation process during experimental immune response in vivo. The use of the optical fluorescence imaging provides a very useful and simple tool to reach this purpose. The motivation comes from the necessity of a robust and simple quantification and data presentation of inflammation based on a vascular permeability. Changes of the fluorescent intensity, as a function of time is a widely accepted method to assess the vascular permeability during inflammation related to the immune response. In the present study we propose to bring a new dimension by applying a more sophisticated approach to the analysis of vascular reaction by using a quantitative analysis based on methods derived from astronomical observations, in particular by using a space-time Fourier filtering analysis followed by a polynomial orthogonal modes decomposition. We demonstrate that temporal evolution of the fluorescent intensity observed at certain pixels correlates quantitatively to the blood flow circulation at normal conditions. The approach allows to determine the regions of permeability and monitor both the fast kinetics related to the contrast material distribution in the circulatory system and slow kinetics associated with extravasation of the contrast material. Thus, we introduce a simple and convenient method for fast quantitative visualization of the leakage related to the inflammatory (immune) reaction in vivo.

  12. The bacterial DNA repair protein Mfd confers resistance to the host nitrogen immune response

    PubMed Central

    Guillemet, Elisabeth; Leréec, Alain; Tran, Seav-Ly; Royer, Corinne; Barbosa, Isabelle; Sansonetti, Philippe; Lereclus, Didier; Ramarao, Nalini

    2016-01-01

    Production of reactive nitrogen species (NO) is a key step in the immune response following infections. NO induces lesions to bacterial DNA, thus limiting bacterial growth within hosts. Using two pathogenic bacteria, Bacillus cereus and Shigella flexneri, we show that the DNA-repair protein Mfd (Mutation-Frequency-Decline) is required for bacterial resistance to the host-NO-response. In both species, a mutant deficient for mfd does not survive to NO, produced in vitro or by phagocytic cells. In vivo, the ∆mfd mutant is avirulent and unable to survive the NO-stress. Moreover, NO induces DNA-double-strand-breaks and point mutations in the Δmfd mutant. In overall, these observations demonstrate that NO damages bacterial DNA and that Mfd is required to maintain bacterial genomic integrity. This unexpected discovery reveals that Mfd, a typical housekeeping gene, turns out to be a true virulence factor allowing survival and growth of the pathogen in its host, due to its capacity to protect the bacterium against NO, a key molecule of the innate immune defense. As Mfd is widely conserved in the bacterial kingdom, these data highlight a mechanism that may be used by a large spectrum of bacteria to overcome the host immune response and especially the mutagenic properties of NO. PMID:27435260

  13. A dual purpose universal influenza vaccine candidate confers protective immunity against anthrax.

    PubMed

    Arévalo, Maria T; Li, Junwei; Diaz-Arévalo, Diana; Chen, Yanping; Navarro, Ashley; Wu, Lihong; Yan, Yongyong; Zeng, Mingtao

    2017-03-01

    Preventive influenza vaccines must be reformulated annually because of antigen shift and drift of circulating influenza viral strains. However, seasonal vaccines do not always match the circulating strains, and there is the ever-present threat that avian influenza viruses may adapt to humans. Hence, a universal influenza vaccine is needed to provide protective immunity against a broad range of influenza viruses. We designed an influenza antigen consisting of three tandem M2e repeats plus HA2, in combination with a detoxified anthrax oedema toxin delivery system (EFn plus PA) to enhance immune responses. The EFn-3×M2e-HA2 plus PA vaccine formulation elicited robust, antigen-specific, IgG responses; and was protective against heterologous influenza viral challenge when intranasally delivered to mice three times. Moreover, use of the detoxified anthrax toxin system as an adjuvant had the additional benefit of generating protective immunity against anthrax. Hence, this novel vaccine strategy could potentially address two major emerging public health and biodefence threats. © 2016 John Wiley & Sons Ltd.

  14. [Construction of combinatorial immune library of single chain human antibodies to orthopoxviruses and selection from this library antibodies to recombinant protein prA30L of variola virus].

    PubMed

    Dubrovskaia, V V; Ulitin, A B; Laman, A G; Gileva, I P; Bormotov, N I; Il'ichev, A A; Brovko, F A; Shchelkunov, S N; Belanov, E F; Tikunova, N V

    2007-01-01

    A combinatorial immune library of human single-chain antibody fragments (scFv) was constructed on the base of genes encoding variable domains of heavy and light chains of immunoglobulins cloned from the lymphocytes of four vaccinia virus (VACV) vaccinated donors. The size of the library was 3 x 10(7) independent clones. After the library was enriched with the clones producing scFv against recombinant analogue of variola virus surface protein prA30L, a panel of unique antibodies specific to both prA30L and VACV was selected from the library. A plaque reduction neutralization test was performed for all selected antibodies and two antibodies were shown to be able to neutralize plaque formation of VACV in Vero E6 cells monolayer. Binding specificities of these antibodies were confirmed using ELISA and Western blot analysis. To determine the amino acid sequences of neutralizing antibodies their genes were sequenced.

  15. Construction of a full-length cDNA library of Solen grandis dunker and identification of defense- and immune-related genes

    NASA Astrophysics Data System (ADS)

    Sun, Guohua; Liu, Xiangquan; Ren, Lihua; Yang, Jianmin; Wei, Xiumei; Yang, Jialong

    2013-11-01

    The basic genetic characteristics, important functional genes, and entire transcriptome of Solen grandis Dunker were investigated by constructing a full-length cDNA library with the `switching mechanism at the 5'-end of the RNA transcript' (SMART) technique. Total RNA was isolated from the immune-relevant tissues, gills and hemocytes, using the Trizol reagent, and cDNA fragments were digested with Sfi I before being ligated to the pBluescript II SK* vector. The cDNA library had a titer of 1048 cfu μL-1 and a storage capacity of 1.05×106 cfu. Approximately 98% of the clones in the library were recombinants, and the fragment lengths of insert cDNA ranged from 0.8 kb to 3.0 kb. A total of 2038 expressed sequence tags were successfully sequenced and clustered into 965 unigenes. BLASTN analysis showed that 240 sequences were highly similar to the known genes (E-value < 1e -5; percent identity >80%), accounting for 25% of the total unigenes. According to the Gene Ontology, these unigenes were related to several biological processes, including cell structure, signal transport, protein synthesis, transcription, energy metabolism, and immunity. Fifteen of the identified sequences were related to defense and immunity. The full-length cDNA sequence of HSC70 was obtained. The cDNA library of S. grandis provided a useful resource for future researches of functional genomics related to stress tolerance, immunity, and other physiological activities.

  16. Information Management. VALA National Conference on Library Automation Proceedings (2nd, Melbourne, Australia, November 28-December 1, 1983). Volume 1.

    ERIC Educational Resources Information Center

    Cheney, B. J., Ed.

    The first of two volumes of a conference proceedings, this document contains the welcome, the opening, the keynote addresses, and the invited papers, as well as a submitted paper by John Gillam which was not available for publication in the pre-prints (Volume 2) prior to the conference. Three addresses are not included in these proceedings and…

  17. Information Management. VALA National Conference on Library Automation Proceedings (2nd, Melbourne, Australia, November 28-December 1, 1983). Volume 1.

    ERIC Educational Resources Information Center

    Cheney, B. J., Ed.

    The first of two volumes of a conference proceedings, this document contains the welcome, the opening, the keynote addresses, and the invited papers, as well as a submitted paper by John Gillam which was not available for publication in the pre-prints (Volume 2) prior to the conference. Three addresses are not included in these proceedings and…

  18. A Report on the 30th Annual Conference of the International Association of Scientific and Technological University Libraries (IATUL)

    ERIC Educational Resources Information Center

    Tripathi, Manorama

    2010-01-01

    Purpose: The paper aims to report on the 30th IATUL Annual Conference held in Leuven, Belgium, 1-4 June, 2009. Design/methodology/approach: The paper summarises the major themes of the conference as well as giving some specific details of developments at the Indira Gandhi National Open University in India, which supplement the author's…

  19. A Report on the 30th Annual Conference of the International Association of Scientific and Technological University Libraries (IATUL)

    ERIC Educational Resources Information Center

    Tripathi, Manorama

    2010-01-01

    Purpose: The paper aims to report on the 30th IATUL Annual Conference held in Leuven, Belgium, 1-4 June, 2009. Design/methodology/approach: The paper summarises the major themes of the conference as well as giving some specific details of developments at the Indira Gandhi National Open University in India, which supplement the author's…

  20. Library and Information Services for Literacy, Productivity and Democracy. Joint Hearing before the Subcommittee on Education, Arts and Humanities of the Committee on Labor and Human Resources, United States Senate, and the Subcommittee on Labor-Management Relations of the Committee on Education and Labor, House of Representatives. One Hundred Second Congress, First Session. Oversight Hearing at the White House Conference on Library and Information Services.

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and Labor.

    The oversight hearing at the second White House Conference on Library and Information Services (WHCLIS2) focuses on the role of the library in three major areas addressed by conference presenters: a literate work force; the productivity to compete in the international marketplace in the 21st century; and a populace fully equipped to participate in…

  1. Immunizations

    MedlinePlus

    ... Loss Surgery? A Week of Healthy Breakfasts Shyness Immunizations KidsHealth > For Teens > Immunizations Print A A A ... That Shot? en español Las vacunas Why Are Vaccinations Important? Measles, mumps, and whooping cough may seem ...

  2. Immunization

    MedlinePlus

    ... a lot worse. Some are even life-threatening. Immunization shots, or vaccinations, are essential. They protect against things like measles, ... B, polio, tetanus, diphtheria, and pertussis (whooping cough). Immunizations are important for adults as well as children. ...

  3. International Federation of Library Associations Annual Conference. Papers of the Special Libraries Division: Administrative, Art, Biological and Medical Libraries Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Tees, Miriam H.; And Others

    This set of nine papers on special library concerns includes papers presented by participants from Canada, Denmark, East Germany, the United States, and the United Kingdom. Papers are entitled: "The Needs of the Special Librarian and the Library Association," by Miriam H. Tees; "Position and Development of Special Libraries within…

  4. IFLA General Conference, 1985. UNESCO/IFLA Presession Seminar. Public Library Service for All: How to Serve Rural Areas. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on public library service to rural areas, which were presented at the 1985 UNESCO/IFLA (United Nations Educational, Scientific, and Cultural Organization/International Federation of Library Associations) presession seminar are compiled here and include: (1) "Characteristics and Needs of Various Groups, Families, and Other Community…

  5. IFLA General Conference, 1985. UNESCO/IFLA Presession Seminar. Public Library Service for All: How to Serve Rural Areas. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on public library service to rural areas, which were presented at the 1985 UNESCO/IFLA (United Nations Educational, Scientific, and Cultural Organization/International Federation of Library Associations) presession seminar are compiled here and include: (1) "Characteristics and Needs of Various Groups, Families, and Other Community…

  6. Innate NKT lymphocytes confer superior adaptive immunity via tumor-capturing dendritic cells.

    PubMed

    Liu, Kang; Idoyaga, Juliana; Charalambous, Anna; Fujii, Shin-Ichiro; Bonito, Anthony; Mordoh, Jose; Wainstok, Rosa; Bai, Xue-Feng; Liu, Yang; Steinman, Ralph M

    2005-12-05

    If irradiated tumor cells could be rendered immunogenic, they would provide a safe, broad, and patient-specific array of antigens for immunotherapies. Prior approaches have emphasized genetic transduction of live tumor cells to express cytokines, costimulators, and surrogate foreign antigens. We asked if immunity could be achieved by delivering irradiated, major histocompatibility complex-negative plasmacytoma cells to maturing mouse dendritic cells (DCs) within lymphoid organs. Tumor cells injected intravenously (i.v.) were captured by splenic DCs, whereas subcutaneous (s.c.) injection led only to weak uptake in lymph node or spleen. The natural killer T (NKT) cells mobilizing glycolipid alpha-galactosyl ceramide, used to mature splenic DCs, served as an effective adjuvant to induce protective immunity. This adjuvant function was mimicked by a combination of poly IC and agonistic alphaCD40 antibody. The adjuvant glycolipid had to be coadministered with tumor cells i.v. rather than s.c. Specific resistance was generated both to a plasmacytoma and lymphoma. The resistance afforded by a single vaccination lasted >2 mo and required both CD4+ and CD8+ T cells. Mature tumor capturing DCs stimulated the differentiation of P1A tumor antigen-specific, CD8+ T cells and uniquely transferred tumor resistance to naive mice. Therefore, the access of dying tumor cells to DCs that are maturing to activated NKT cells efficiently induces long-lived adaptive resistance.

  7. Adiponectin confers protection from acute colitis and restricts a B cell immune response.

    PubMed

    Obeid, Stephanie; Wankell, Miriam; Charrez, Berenice; Sternberg, Jade; Kreuter, Roxane; Esmaili, Saeed; Ramezani-Moghadam, Mehdi; Devine, Carol; Read, Scott; Bhathal, Prithi; Lopata, Andreas; Ahlensteil, Golo; Qiao, Liang; George, Jacob; Hebbard, Lionel

    2017-04-21

    Adiponectin demonstrates beneficial effects in various metabolic diseases, including diabetes, and in bowel cancer. Recent data also suggest a protective role in colitis. However, the precise molecular mechanisms by which adiponectin and its receptors modulate colitis and the nature of the adaptive immune response in murine models are yet to be elucidated. Adiponectin knock-out mice were orally administered dextran sulfate sodium for 7 days and were compared with wild-type mice. The severity of disease was analyzed histopathologically and through cytokine profiling. HCT116 colonic epithelial cells were employed to analyze the in vitro effects of adiponectin and AdipoR1 interactions in colonic injury following dextran sulfate sodium treatment. Adiponectin knock-out mice receiving dextran sulfate sodium exhibited severe colitis, had greater inflammatory cell infiltration, and an increased presence of activated B cells compared with controls. This was accompanied by an exaggerated proinflammatory cytokine profile and increased STAT3 signaling. Adiponectin knock-out mouse colons had markedly reduced proliferation and increased epithelial apoptosis and cellular stress. In vitro, adiponectin reduced apoptotic, anti-proliferative, and stress signals and restored STAT3 signaling. Following the abrogation of AdipoR1 in vitro, these protective effects of adiponectin were abolished. In summary, adiponectin maintains intestinal homeostasis and protects against murine colitis through interactions with its receptor AdipoR1 and by modulating adaptive immunity. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  8. Laser-induced immune modulation inhibits tumor growth in vivo (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Ottaviani, Giulia; Martinelli, Valentina; Rupel, Katia; Caronni, Nicoletta; Naseem, Asma; Zandonà, Lorenzo; Perinetti, Giuseppe; Gobbo, Margherita; Di Lenarda, Roberto; Bussani, Rossana; Benvenuti, Federica; Giacca, Mauro; Biasotto, Matteo; Zacchigna, Serena

    2017-02-01

    Photobiomodulation stands as a recommended therapy for oral mucositis induced by oncological therapies. However, its mechanisms of action and, more importantly, its safety in cancer patients, are still unclear. We assessed cancer cell metabolism and proliferation in vitro and in vivo after exposure to different laser protocols. We exploited both ectopic melanoma and a more physiological oral carcinogenesis mouse model, followed by molecular, histological and immunohistochemical characterization. Laser irradiation resulted in a slightly increase in cell metabolism and proliferation in vitro, albeit each protocol exerted a difference response. Of notice, in vivo laser light reduced tumour growth and invasiveness, indicating e beneficial effect on tumor microenvironment. Laser-treated tumors were surrounded and infiltrated by immune cells, mainly lymphocytes and dendritic cells, paralleled by an enhanced secretion of type I interferons. In contrast, the number of pro-angiogenic macrophages was reduced in response to laser irradiation, with consequent normalization of the tumor vasculature. Based on these finding we have also started exploring the effect of photobiomodulation on lymphocyte response in an experimental model of vaccination. Preliminary data indicate that laser light induced antigen-specific CD8+ and CD4+ T cell responses. In conclusion, our data point toward photobiomodulation as an effective strategy to boost the immune response in vivo, with relevant, therapeutic activities in both cancer and vaccination experimental models. These results support the safe use of laser light on cancer patients and open the way to innovative therapeutic opportunities.

  9. Human Virus-Derived Small RNAs Can Confer Antiviral Immunity in Mammals.

    PubMed

    Qiu, Yang; Xu, Yanpeng; Zhang, Yao; Zhou, Hui; Deng, Yong-Qiang; Li, Xiao-Feng; Miao, Meng; Zhang, Qiang; Zhong, Bo; Hu, Yuanyang; Zhang, Fu-Chun; Wu, Ligang; Qin, Cheng-Feng; Zhou, Xi

    2017-06-20

    RNA interference (RNAi) functions as a potent antiviral immunity in plants and invertebrates; however, whether RNAi plays antiviral roles in mammals remains unclear. Here, using human enterovirus 71 (HEV71) as a model, we showed HEV71 3A protein as an authentic viral suppressor of RNAi during viral infection. When the 3A-mediated RNAi suppression was impaired, the mutant HEV71 readily triggered the production of abundant HEV71-derived small RNAs with canonical siRNA properties in cells and mice. These virus-derived siRNAs were produced from viral dsRNA replicative intermediates in a Dicer-dependent manner and loaded into AGO, and they were fully active in degrading cognate viral RNAs. Recombinant HEV71 deficient in 3A-mediated RNAi suppression was significantly restricted in human somatic cells and mice, whereas Dicer deficiency rescued HEV71 infection independently of type I interferon response. Thus, RNAi can function as an antiviral immunity, which is induced and suppressed by a human virus, in mammals. Copyright © 2017 Elsevier Inc. All rights reserved.

  10. Design and construction of immune phage antibody library against Tetanus neurotoxin: Production of single chain antibody fragments.

    PubMed

    Sadreddini, Sanam; Seifi-Najmi, Mehrnosh; Ghasemi, Babollah; Kafil, Hossein Samadi; Alinejad, Vahideh; Sadreddini, Sevil; Younesi, Vahid; Jadidi-Niaragh, Farhad; Yousefi, Mehdi

    2015-12-23

    Tetanus neurotoxin (TeNT) is composed of a light (LC) and heavy chain (HC) polypeptides, released by anaerobic bacterium Clostridium tetani and can cause fatal life-threatening infectious disease. Toxin HC and LC modules represents receptor binding and zinc metalloprotease activity, respectively. The passive administration of animal-derived antibodies against tetanus toxin has been considered as the mainstay therapy for years. However, this treatment is associated with several adverse effects due to the presence of anti-isotype antibodies. In the present study, we have produced the fully human single chain antibody fragments (HuScFv) from two human antibody phage display libraries. Twenty-four different HuscFvs were isolated from two anti TeNT immune libraries. Our produced human ScFv (HuScFv) were converted to IgG platform and analyzed regarding their specific reactivity to TeNT. All of the selected scFvs have the same VL but different VH. Three HuscFvs from the first library (TTX15, 51, 75) and two HuscFvs from the second library (TTX16, 20) were chosen to convert to IgG1 using pOptiVEC and pcDNA3.3 systems. Production of IgG1 from transfected DG44 and binding capacity of them to tetanus toxin and toxoid were measured by ELISA. ELISA results showed no detectable production of TTX16 and TTX20 IgG1. Although, TTX51 and TTX75 were converted and produced as IgG1, no reactivity to tetanus toxin and toxoid was observed. However, TTX15 was successfully produced as whole IgG1 platform with reactivity to both tetanus toxin and toxoid. The latter would be an appropriate replacement for conventional polyclonal antibodies if would meet the further characterization including specificity determination, affinity measurement and toxin neutralizing assays. Our results demonstrated production of functional IgG1 derived from TTX15 scFv and might be an appropriate replacement for polyclonal Tetabulin but it needs further characterization.

  11. Instruction in the Use of the College and University Library: Selected Conference Papers [from] July 13-14, 1970 Conference/Workshop.

    ERIC Educational Resources Information Center

    California Univ., Berkeley. School of Librarianship.

    Library programs at U.C. Berkeley, San Jose State College and U.C.L.A. are presented together with an introduction by Associate Professor Harlan, U.C. School of Librarianship. At U.C. Berkeley the experimental program has involved twenty-two librarian-instructors and has reached 825 students. At San Jose, a "team teaching" approach involving the…

  12. Isolation and characterisation of Ebolavirus-specific recombinant antibody fragments from murine and shark immune libraries.

    PubMed

    Goodchild, Sarah A; Dooley, Helen; Schoepp, Randal J; Flajnik, Martin; Lonsdale, Stephen G

    2011-09-01

    Members of the genus Ebolavirus cause fulminating outbreaks of disease in human and non-human primate populations with a mortality rate up to 90%. To facilitate rapid detection of these pathogens in clinical and environmental samples, robust reagents capable of providing sensitive and specific detection are required. In this work recombinant antibody libraries were generated from murine (single chain variable domain fragment; scFv) and nurse shark, Ginglymostoma cirratum (IgNAR V) hosts immunised with Zaire ebolavirus. This provides the first recorded IgNAR V response against a particulate antigen in the nurse shark. Both murine scFv and shark IgNAR V libraries were panned by phage display technology to identify useful antibodies for the generation of immunological detection reagents. Two murine scFv were shown to have specificity to the Zaire ebolavirus viral matrix protein VP40. Two isolated IgNAR V were shown to bind to the viral nucleoprotein (NP) and to capture viable Zaire ebolavirus with a high degree of sensitivity. Assays developed with IgNAR V cross-reacted to Reston ebolavirus, Sudan ebolavirus and Bundibugyo ebolavirus. Despite this broad reactivity, neither of IgNAR V showed reactivity to Côte d'Ivoire ebolavirus. IgNAR V was substantially more resistant to irreversible thermal denaturation than murine scFv and monoclonal IgG in a comparative test. The demonstrable robustness of the IgNAR V domains may offer enhanced utility as immunological detection reagents in fieldable biosensor applications for use in tropical or subtropical countries where outbreaks of Ebolavirus haemorrhagic fever occur.

  13. Near infrared photoimmunotherapy rapidly elicits specific host immunity against cancer cells (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Kobayashi, Hisataka

    2017-02-01

    Near infrared photoimmunotherapy (NIR-PIT) is a new molecularly-targeted cancer photo-therapy based on conjugating a near infrared silica-phthalocyanine dye, IR700, to a monoclonal antibody (mAb) targeting cell-surface molecules. When exposed to NIR light, the conjugate induces a highly-selective necrotic/immunogenic cell death (ICD) only in target-positive, mAb-IR700-bound cancer cells. This cell death occurs as early as 1 minute after exposure to NIR light. Meanwhile, immediately adjacent target-negative cells are unharmed. Dynamic 3D-microscopy of live tumor cells undergoing NIR-PIT showed rapid swelling in treated cells immediately after light exposure, followed by irreversible morphologic changes such as bleb formation, and rupture of vesicles within several minutes. Furthermore, biological markers of ICD including relocation of HSP70/90 and calreticulin, and release of ATP and High Mobility Group Box 1 (HMGB1), were clearly detected immediately after NIR-PIT. When NIR-PIT was performed in a mixture of cancer cells and immature dendritic cells, maturation of immature dendritic cells was strongly induced rapidly after NIR-PIT. Alternatively, NIR-PIT can also target negative regulatory immune cells such as Treg only in the tumor bed. Treg targeting NIR-PIT against CD25 can deplete >80% of Treg in tumor bed within 20 min that induces activation of tumor cell-specific CD8+-T and NK cells within 1.5 hour, and then these activated cells killed cancer cells in local tumor within 1 day and also in distant tumors of the same cell origin within 2 days. In summary, cancer cell-targeting and immuno-suppressor cell-targeting NIR-PITs effectively induce innate and acquired immunity specifically against cancer cells growing in patients, respectively.

  14. DOCK2 confers immunity and intestinal colonization resistance to Citrobacter rodentium infection

    PubMed Central

    Liu, Zhiping; Man, Si Ming; Zhu, Qifan; Vogel, Peter; Frase, Sharon; Fukui, Yoshinori; Kanneganti, Thirumala-Devi

    2016-01-01

    Food poisoning is one of the leading causes of morbidity and mortality in the world. Citrobacter rodentium is an enteric pathogen which attaches itself to enterocytes and induces attachment and effacing (A/E) lesions. The ability of the bacterium to cause infection requires subversion of the host actin cytoskeleton. Rac-dependent actin polymerization is activated by a guanine nucleotide exchange factor known as Dedicator of cytokinesis 2 (DOCK2). However, the role of DOCK2 in infectious disease is largely unexplored. Here, we found that mice lacking DOCK2 were susceptible to C. rodentium infection. These mice harbored increased levels of C. rodentium bacteria, showed more pronounced weight loss and inflammation-associated pathology, and were prone to bacterial dissemination to the systemic organs compared with wild-type mice. We found that mice lacking DOCK2 were more susceptible to C. rodentium attachment to intestinal epithelial cells. Therefore, our results underscored an important role of DOCK2 for gastrointestinal immunity to C. rodentium infection. PMID:27291827

  15. IFLA General Conference, 1990. Division of Libraries Serving the General Public: Section on Public Libraries; Section of Children's Libraries, Children's Literature Documentation Centres, Round Table; Section of School Libraries; Section of Libraries for the Blind; INTAMEL, Round Table; Mobile Libraries, Round Table. Booklet 3.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 28 papers in this collection were presented at meetings of four sections and three round tables within the Division of Libraries Serving the General Public: (1) "Rural Community Information Services: Guidelines for Researching Need, Setting Up Services and Evaluating Performance" (Elaine Kempson); (2) "Library Activities at the Workplace"…

  16. IFLA General Conference, 1990. Division of Libraries Serving the General Public: Section on Public Libraries; Section of Children's Libraries, Children's Literature Documentation Centres, Round Table; Section of School Libraries; Section of Libraries for the Blind; INTAMEL, Round Table; Mobile Libraries, Round Table. Booklet 3.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 28 papers in this collection were presented at meetings of four sections and three round tables within the Division of Libraries Serving the General Public: (1) "Rural Community Information Services: Guidelines for Researching Need, Setting Up Services and Evaluating Performance" (Elaine Kempson); (2) "Library Activities at the Workplace"…

  17. 1(st) EMBL/DFG Women in Science Network Conference Heidelberg 2016: From Genes, Cells and the Immune System towards Therapies - Meeting Report.

    PubMed

    Stripecke, Renata; Gouttefangeas, Cécile; Förster, Irmgard

    2016-11-01

    The 1(st) EMBL/DFG Women in Science (WiS) Conference "From Genes, Cells and the Immune System towards Therapies" was held on 19(th) - 20(th) September 2016 in Heidelberg, Germany. The WiS conference was funded by nine Collaborative Research Centers (CRCs) of the German Research Council (Deutsche Forschungsgemeinschaft, DFG; Table 1) and benefited from an outstanding hosting environment at the Advanced Training Center of the European Molecular Biology Laboratory (EMBL). Scientific talks focused at genetic, cellular and immunologic mechanisms, and immune therapy, and progress from all stages of development covering basic research to clinical developments was described. The presentations were embedded between structured networking sessions and a round table discussion with representatives of the DFG, EMBL, European Molecular Biology Organisation (EMBO), and the German Society of Immunology (DGfI).

  18. Recombinant Toxoplasma gondii phosphoglycerate mutase 2 confers protective immunity against toxoplasmosis in BALB/c mice.

    PubMed

    Wang, Hai-Long; Wen, Li-Min; Pei, Yan-Jiang; Wang, Fen; Yin, Li-Tian; Bai, Ji-Zhong; Guo, Rui; Wang, Chun-Fang; Yin, Guo-Rong

    2016-01-01

    Toxoplasmosis is one of the most widespread zoonoses worldwide. It has a high incidence and can result in severe disease in humans and livestock. Effective vaccines are needed to limit and prevent infection with Toxoplasma gondii. In this study, we evaluated the immuno-protective efficacy of a recombinant Toxoplasma gondii phosphoglycerate mutase 2 (rTgPGAM 2) against T. gondii infection in BALB/c mice. We report that the mice nasally immunised with rTgPGAM 2 displayed significantly higher levels of special IgG antibodies against rTgPGAM 2 (including IgG1, IgG2a and IgAs) and cytokines (including IFN-γ, IL-2 and IL-4) in their blood sera and supernatant of cultured spleen cells compared to those of control animals. In addition, an increased number of spleen lymphocytes and enhanced lymphocyte proliferative responses were observed in the rTgPGAM 2-immunised mice. After chronic infection and lethal challenge with the highly virulent T. gondii RH strain by oral gavage, the survival time of the rTgPGAM 2-immunised mice was longer (P < 0.01) and the survival rate (70%) was higher compared with the control mice (P < 0.01). The reduction rate of brain and liver tachyzoites in rTgPGAM 2-vaccinated mice reached approximately 57% and 69% compared with those of the control mice (P < 0.01). These results suggest that rTgPGAM 2 can generate protective immunity against T. gondii infection in BALB/c mice and may be a promising antigen in the further development of an effective vaccine against T. gondii infection. © H.-L. Wang et al., published by EDP Sciences, 2016.

  19. Immunization with Fasciola hepatica thioredoxin glutathione reductase failed to confer protection against fasciolosis in cattle.

    PubMed

    Maggioli, Gabriela; Bottini, Gualberto; Basika, Tatiana; Alonzo, Pablo; Salinas, Gustavo; Carmona, Carlos

    2016-07-15

    The liver fluke Fasciola hepatica remains an important agent of food-borne trematode disease producing great economic losses due to its negative effect on productivity of livestock grazing in temperate areas. The prevailing control strategy based on anthelmintic drugs is unsustainable due to widespread resistance hence vaccination appears as an attractive option to pursue. In this study we evaluate the effect of vaccination in calves with a functional recombinant thioredoxin glutathione reductase (rFhTGR) from liver fluke, a critical antioxidant enzyme at the crossroads of the thioredoxin and glutathione metabolism in flatworms. The recombinant enzyme produced in Escherichia coli was tested in two vaccination experiments; in the first trial rFhTGR was administered in combination with Freund́s Incomplete Adjuvant (FIA) in a three-inoculation scheme on weeks 0, 4 and 8; in the second trial rFhTGR was given mixed with Adyuvac 50 or Alum as adjuvants on weeks 0 and 4. In both cases calves were challenged with metacercariae (400 in the first and 500 in the second trial) 2 weeks after the last inoculation. Our results demonstrate that two or three doses of the vaccine induced a non-significant reduction in worm counts of 8.2% (FIA), 3.8% (Adyuvac 50) and 23.0% (Alum) compared to adjuvant controls indicating that rFhTGR failed to induce a protective immunity in challenged calves. All vaccine formulations induced a mixed IgG1/IgG2 response but no booster was observed after challenge. No correlations between antibody titres and worm burdens were found. Copyright © 2016 Elsevier B.V. All rights reserved.

  20. Protective immunity conferred by porcine circovirus 2 ORF2-based DNA vaccine in mice.

    PubMed

    Sylla, Seydou; Cong, Yan-Long; Sun, Yi-Xue; Yang, Gui-Lian; Ding, Xue-Mei; Yang, Zhan-Qing; Zhou, Yu-Long; Yang, Minnan; Wang, Chun-Feng; Ding, Zhuang

    2014-07-01

    Post-weaning multisystemic wasting syndrome (PMWS) associated with porcine circovirus type 2 (PCV2) has caused the swine industry significant health challenges and economic damage. Although inactivated and subunit vaccines against PMWS have been used widely, so far no DNA vaccine is available. In this study, with the aim of exploring a new route for developing a vaccine against PCV2, the immunogenicity of a DNA vaccine was evaluated in mice. The pEGFP-N1 vector was used to construct a PCV2 Cap gene recombinant vaccine. To assess the immunogenicity of pEGFP-Cap, 80 BALB/c mice were immunized three times at 2 weekly intervals with pEGFP-Cap, LG-strain vaccine, pEGFP-N1 vector or PBS and then challenged with PCV2. IgG and cytokines were assessed by indirect ELISA and ELISA, respectively. Specimens stained with hematoxylin and eosin (HE) and immunohistochemistry (IHC) techniques were examined histopathologically. It was found that vaccination of the mice with the pEGFP-Cap induced solid protection against PCV2 infection through induction of highly specific serum IgG antibodies and cytokines (IFN-γ and IL-10), and a small PCV2 viral load. The mice treated with the pEGFP-Cap and LG-strain developed no histopathologically detectable lesions (HE stain) and IHC techniques revealed only a few positive cells. Thus, this study demonstrated that recombinant pEGFP-Cap substantially alleviates PCV2 infection in mice and provides evidence that a DNA vaccine could be an alternative to PCV2 vaccines against PMWS. © 2014 The Societies and Wiley Publishing Asia Pty Ltd.

  1. Recombinant Toxoplasma gondii phosphoglycerate mutase 2 confers protective immunity against toxoplasmosis in BALB/c mice

    PubMed Central

    Wang, Hai-Long; Wen, Li-Min; Pei, Yan-Jiang; Wang, Fen; Yin, Li-Tian; Bai, Ji-Zhong; Guo, Rui; Wang, Chun-Fang; Yin, Guo-Rong

    2016-01-01

    Toxoplasmosis is one of the most widespread zoonoses worldwide. It has a high incidence and can result in severe disease in humans and livestock. Effective vaccines are needed to limit and prevent infection with Toxoplasma gondii. In this study, we evaluated the immuno-protective efficacy of a recombinant Toxoplasma gondii phosphoglycerate mutase 2 (rTgPGAM 2) against T. gondii infection in BALB/c mice. We report that the mice nasally immunised with rTgPGAM 2 displayed significantly higher levels of special IgG antibodies against rTgPGAM 2 (including IgG1, IgG2a and IgAs) and cytokines (including IFN-γ, IL-2 and IL-4) in their blood sera and supernatant of cultured spleen cells compared to those of control animals. In addition, an increased number of spleen lymphocytes and enhanced lymphocyte proliferative responses were observed in the rTgPGAM 2-immunised mice. After chronic infection and lethal challenge with the highly virulent T. gondii RH strain by oral gavage, the survival time of the rTgPGAM 2-immunised mice was longer (P < 0.01) and the survival rate (70%) was higher compared with the control mice (P < 0.01). The reduction rate of brain and liver tachyzoites in rTgPGAM 2-vaccinated mice reached approximately 57% and 69% compared with those of the control mice (P < 0.01). These results suggest that rTgPGAM 2 can generate protective immunity against T. gondii infection in BALB/c mice and may be a promising antigen in the further development of an effective vaccine against T. gondii infection. PMID:26984115

  2. IFLA General Conference, 1992. Division of Management and Technology: Audiovisual Media (RT); Section on Library Services to Multicultural Populations; Section on Library Buildings and Equipment; Section on Information Technology; Management of Library Associations (RT); Section on Statistics. Papers

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, London (England).

    Eleven papers delivered at the annual meeting of the International Federation of Library Associations and Institutions for the Division of Management and Technology are presented. Some were presented at a roundtable on audiovisual media, and others are from sessions on library buildings and equipment, information management, and statistics in…

  3. Neutralization Analysis of a Chicken Single-Chain Variable Fragment Derived from an Immune Antibody Library Against Infectious Bronchitis Virus.

    PubMed

    Lin, Yuan; Li, Benqiang; Ye, Jiaxin; Wang, Man; Wang, Jianhua; Zhang, Ying; Zhu, Jianguo

    2015-09-01

    Avian infectious bronchitis virus (IBV), which is prevalent in many countries causing severe economic loss to the poultry industry, causes infectious bronchitis (IB) in birds. Recombinant single-chain variable fragments (scFvs) have been proven to effectively inhibit many viruses, both in vitro and in vivo, and they could be a potential diagnostic and therapeutic reagent to control IB. In this study, six anti-IBV chicken scFvs, ZL.10, ZL.64, ZL.78, ZL.80, ZL.138, and ZL.256, were obtained by screening random clones from an immune antibody library. An analysis of nucleotide sequences revealed that they represented distinctive genetic sequences and greatly varied in complementarity-determining region three of the heavy chain. Neutralization tests showed that ZL.10, which bound the S1 protein in western blots, inhibited the formation of syncytia in Vero cells 48 h post IBV infection and decreased the transcriptional level of nucleoprotein mRNA to 17.2%, while the other five scFvs, including ZL.78 and ZL.256, that bound the N protein did not. In conclusion, the results suggested that specific and neutralizing chicken scFvs against IBV, which can be safe and economical antibody reagents, can be produced in vitro through prokaryotic expression.

  4. Tomato I2 Immune Receptor Can Be Engineered to Confer Partial Resistance to the Oomycete Phytophthora infestans in Addition to the Fungus Fusarium oxysporum.

    PubMed

    Giannakopoulou, Artemis; Steele, John F C; Segretin, Maria Eugenia; Bozkurt, Tolga O; Zhou, Ji; Robatzek, Silke; Banfield, Mark J; Pais, Marina; Kamoun, Sophien

    2015-12-01

    Plants and animals rely on immune receptors, known as nucleotide-binding domain and leucine-rich repeat (NLR)-containing proteins, to defend against invading pathogens and activate immune responses. How NLR receptors respond to pathogens is inadequately understood. We previously reported single-residue mutations that expand the response of the potato immune receptor R3a to AVR3a(EM), a stealthy effector from the late blight oomycete pathogen Phytophthora infestans. I2, another NLR that mediates resistance to the will-causing fungus Fusarium oxysporum f. sp. lycopersici, is the tomato ortholog of R3a. We transferred previously identified R3a mutations to I2 to assess the degree to which the resulting I2 mutants have an altered response. We discovered that wild-type I2 protein responds weakly to AVR3a. One mutant in the N-terminal coiled-coil domain, I2(I141N), appeared sensitized and displayed markedly increased response to AVR3a. Remarkably, I2(I141N) conferred partial resistance to P. infestans. Further, I2(I141N) has an expanded response spectrum to F. oxysporum f. sp. lycopersici effectors compared with the wild-type I2 protein. Our results suggest that synthetic immune receptors can be engineered to confer resistance to phylogenetically divergent pathogens and indicate that knowledge gathered for one NLR could be exploited to improve NLR from other plant species.

  5. EPIVAC International Conference on Financial Sustainability of Immunization Programs in sub-Saharan Africa, February 16-18, 2012, Ouidah, Benin.

    PubMed

    Drach, Marcel; Le Gargasson, Jean-Bernard; Mathonnat, Jacky; Da Silva, Alfred; Kaddar, Miloud; Colombini, Anaïs

    2013-09-23

    The introduction of new vaccines with much higher prices than traditional vaccines results in increasing budgetary pressure on immunization programs in GAVI-eligible countries, increasing the need to ensure their financial sustainability. In this context, the third EPIVAC (Epidemiology and Vaccinology) technical conference was held from February 16 to 18, 2012 at the Regional Institute of Public Health in Ouidah, Benin. Managers of ministries of health and finance from 11 West African countries (GAVI eligible countries), as well as former EPIVAC students and European experts, shared their knowledge and best practices on immunization financing at district and country level. The conference concluded by stressing five major priorities for the financial sustainability of national immunization programs (NIPs) in GAVI-eligible countries. - Strengthen public financing by increasing resources and fiscal space, improving budget processes, increasing contribution of local governments and strengthen efficiency of budget spending. - Promote equitable community financing which was recognized as a significant and essential contribution to the continuity of EPI operations. - Widen private funding by exploring prospects offered by sponsorship through foundations dedicated to immunization and by corporate social responsibility programs. - Contain the potential crowding-out effect of GAVI co-financing and ensure that decisions on new vaccine introductions are evidence-based. - Seek out innovative financing mechanisms such as taxes on food products or a national solidarity fund.

  6. International Federation of Library Associations Annual Conference. Plenary Meeting (47th, Leipzig, East Germany, August 17, 1981).

    ERIC Educational Resources Information Center

    Granheim, Else; And Others

    This group of papers from the International Federation of Library Associations (IFLA) comprises six papers presented at the plenary session and two papers from the Bibliographic Control Division of IFLA. Presented at the plenary session were (1) the opening address by the President of IFLA; (2) brief reports from sections and round tables of the…

  7. Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library.

    PubMed

    Rahumatullah, Anizah; Ahmad, Azimah; Noordin, Rahmah; Lim, Theam Soon

    2015-10-01

    Phage display technology is an important tool for antibody generation or selection. This study describes the development of a scFv library and the subsequent analysis of identified monoclonal antibodies against BmSXP, a recombinant antigen for lymphatic filariasis. The immune library was generated from blood of lymphatic filariasis infected individuals. A TA based intermediary cloning approach was used to increase cloning efficiency for the library construction process. A diverse immune scFv library of 10(8) was generated. Six unique monoclonal antibodies were identified from the 50 isolated clones against BmSXP. Analysis of the clones showed a bias for the IgHV3 and Vκ1 (45.5%) and IgHV2 and Vκ3 (27.3%) gene family. The most favored J segment for light chain is IgKJ1 (45.5%). The most favored D and J segment for heavy chain are IgHD6-13 (75%) and IgHJ3 (47.7%). The information may suggest a predisposition of certain V genes in antibody responses against lymphatic filariasis.

  8. Emerging Frameworks and Methods. Proceedings of the International Conference on Conceptions of Library and Information Science (CoLIS4) (4th, Seattle, Washington, July 21-25, 2002).

    ERIC Educational Resources Information Center

    Bruce, Harry, Ed.; Fidel, Raya, Ed.; Ingwersen, Peter, Ed.; Vakkari, Pertti, Ed.

    These proceedings are the fourth in the series of international conferences whose general aim is to provide a broad forum for critically exploring and analyzing library and information science as a discipline and as a field of research from historical, theoretical, philosophical, and empirical perspectives. The papers in this volume cover a wide…

  9. Emerging Frameworks and Methods. Proceedings of the International Conference on Conceptions of Library and Information Science (CoLIS4) (4th, Seattle, Washington, July 21-25, 2002).

    ERIC Educational Resources Information Center

    Bruce, Harry, Ed.; Fidel, Raya, Ed.; Ingwersen, Peter, Ed.; Vakkari, Pertti, Ed.

    These proceedings are the fourth in the series of international conferences whose general aim is to provide a broad forum for critically exploring and analyzing library and information science as a discipline and as a field of research from historical, theoretical, philosophical, and empirical perspectives. The papers in this volume cover a wide…

  10. IFLA General Conference, 1984. Management and Technology Division. Section on Information Technology and Joint Meeting of the Round Table Audiovisual Media, the International Association for Sound Archives, and the International Association for Music Libraries. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Six papers on information technology, the development of information systems for Third World countries, handling of sound recordings, and library automation were presented at the 1984 IFLA conference. They include: (1) "Handling, Storage and Preservation of Sound Recordings under Tropical and Subtropical Climatic Conditions" (Dietrich…

  11. PIALA '96. Jaketo Jaketak Kobban Alele Eo--Identifying, Using and Sharing Local Resources. Proceedings of the Annual Pacific Islands Association of Libraries and Archives Conference (6th, Majuro, Marshall Islands, November 5-8, 1996).

    ERIC Educational Resources Information Center

    Cohen, Arlene, Ed.

    This 1996 PIALA conference explores ways to identify and make available local resources on the Marshall Islands. The traditional Marshallese word, "Alele," which means "the basket which holds the tools, treasures and resources needed for everyday life," is also the name of Majuro's public library, museum and Marshall Islands…

  12. Development of neutralizing scFv-Fc against botulinum neurotoxin A light chain from a macaque immune library.

    PubMed

    Miethe, Sebastian; Rasetti-Escargueil, Christine; Liu, Yvonne; Chahboun, Siham; Pelat, Thibaut; Avril, Arnaud; Frenzel, André; Schirrmann, Thomas; Thullier, Philippe; Sesardic, Dorothea; Hust, Michael

    2014-01-01

    Botulinum toxins (BoNTs) are among the most toxic substances on earth, with serotype A toxin being the most toxic substance known. They are responsible for human botulism, a disease characterized by flaccid muscle paralysis that occurs naturally through food poisoning or the colonization of the gastrointestinal tract by BoNT-producing clostridia. BoNT has been classified as a category A agent by the Centers for Disease Control, and it is one of six agents with the highest potential risk of use as bioweapons. Human or human-like neutralizing antibodies are thus required for the development of anti-botulinum toxin drugs to deal with this possibility. In this study, Macaca fascicularis was hyperimmunized with a recombinant light chain of BoNT/A. An immune phage display library was constructed and, after multistep panning, several scFv with nanomolar affinities that inhibited the endopeptidase activity of BoNT/A1 in vitro as scFv-Fc, with a molar ratio (ab binding site:toxin) of up to 1:1, were isolated. The neutralization of BoNT/A-induced paralysis by the SEM120-IID5, SEM120-IIIC1 and SEM120-IIIC4 antibodies was demonstrated in mouse phrenic nerve-hemidiaphragm preparations with the holotoxin. The neutralization observed is the strongest ever measured in the phrenic nerve-hemidiaphragm assay for BoNT/A1 for a monoclonal antibody. Several scFv-Fc inhibiting the endopeptidase activity of botulinum neurotoxin A were isolated. For SEM120-IID5, SEM120-IIIC1, and SEM120-IIIC4, inhibitory effects in vitro and protection against the toxin ex vivo were observed. The human-like nature of these antibodies makes them promising lead candidates for further development of immunotherapeutics for this disease.

  13. Development of neutralizing scFv-Fc against botulinum neurotoxin A light chain from a macaque immune library

    PubMed Central

    Miethe, Sebastian; Rasetti-Escargueil, Christine; Liu, Yvonne; Chahboun, Siham; Pelat, Thibaut; Avril, Arnaud; Frenzel, Andrè; Schirrmann, Thomas; Thullier, Philippe; Sesardic, Dorothea; Hust, Michael

    2014-01-01

    Botulinum toxins (BoNTs) are among the most toxic substances on earth, with serotype A toxin being the most toxic substance known. They are responsible for human botulism, a disease characterized by flaccid muscle paralysis that occurs naturally through food poisoning or the colonization of the gastrointestinal tract by BoNT-producing clostridia. BoNT has been classified as a category A agent by the Centers for Disease Control, and it is one of six agents with the highest potential risk of use as bioweapons. Human or human-like neutralizing antibodies are thus required for the development of anti-botulinum toxin drugs to deal with this possibility. In this study, Macaca fascicularis was hyperimmunized with a recombinant light chain of BoNT/A. An immune phage display library was constructed and, after multistep panning, several scFv with nanomolar affinities that inhibited the endopeptidase activity of BoNT/A1 in vitro as scFv-Fc, with a molar ratio (ab binding site:toxin) of up to 1:1, were isolated. The neutralization of BoNT/A-induced paralysis by the SEM120-IID5, SEM120-IIIC1 and SEM120-IIIC4 antibodies was demonstrated in mouse phrenic nerve-hemidiaphragm preparations with the holotoxin. The neutralization observed is the strongest ever measured in the phrenic nerve-hemidiaphragm assay for BoNT/A1 for a monoclonal antibody. Several scFv-Fc inhibiting the endopeptidase activity of botulinum neurotoxin A were isolated. For SEM120-IID5, SEM120-IIIC1, and SEM120-IIIC4, inhibitory effects in vitro and protection against the toxin ex vivo were observed. The human-like nature of these antibodies makes them promising lead candidates for further development of immunotherapeutics for this disease. PMID:24492304

  14. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    In "How Special Libraries Use Networks," a paper presented at the 1982 International Federation of Library Associations (IFLA) conference, David R. Bender (United States) advocates cooperation among academic, public, school, and special libraries in the areas of information management, staff services, and acquisition of technology. He…

  15. Immunization.

    ERIC Educational Resources Information Center

    Guerin, Nicole; And Others

    1986-01-01

    Contents of this double journal issue concern immunization and primary health care of children. The issue decribes vaccine storage and sterilization techniques, giving particular emphasis to the role of the cold chain, i.e., the maintenance of a specific temperature range to assure potency of vaccines as they are moved from a national storage…

  16. Immunization.

    ERIC Educational Resources Information Center

    Guerin, Nicole; And Others

    1986-01-01

    Contents of this double journal issue concern immunization and primary health care of children. The issue decribes vaccine storage and sterilization techniques, giving particular emphasis to the role of the cold chain, i.e., the maintenance of a specific temperature range to assure potency of vaccines as they are moved from a national storage…

  17. The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis

    PubMed Central

    Silva, Éverton F.; Medeiros, Marco A.; McBride, Alan J. A.; Matsunaga, Jim; Esteves, Gabriela S.; Ramos, João G. R.; Santos, Cleiton S.; Croda, Júlio; Homma, Akira; Dellagostin, Odir A.; Haake, David A.; Reis, Mitermayer G.; Ko, Albert I.

    2007-01-01

    Subunit vaccines are a potential intervention strategy against leptospirosis, which is a major public health problem in developing countries and a veterinary disease in livestock and companion animals worldwide. Leptospiral immunoglobulin-like (Lig) proteins are a family of surface-exposed determinants that have Ig-like repeat domains found in virulence factors such as intimin and invasin. We expressed fragments of the repeat domain regions of LigA and LigB from Leptospira interrogans serovar Copenhageni. Immunization of Golden Syrian hamsters with Lig fragments in Freund’s adjuvant induced robust antibody responses against recombinant protein and native protein, as detected by ELISA and immunoblot, respectively. A single fragment, LigANI, which corresponds to the six carboxy-terminal Ig-like repeat domains of the LigA molecule, conferred immunoprotection against mortality (67-100%, P <0.05) in hamsters which received a lethal inoculum of L. interrogans serovar Copenhageni. However, immunization with this fragment did not confer sterilizing immunity. These findings indicate that the carboxy-terminal portion of LigA is an immunoprotective domain and may serve as a vaccine candidate for human and veterinary leptospirosis. PMID:17629368

  18. Passive immunization with a polyclonal antiserum to the hemoglobin receptor of Haemophilus ducreyi confers protection against a homologous challenge in the experimental swine model of chancroid.

    PubMed

    Leduc, Isabelle; Fusco, William G; Choudhary, Neelima; Routh, Patty A; Cholon, Deborah M; Hobbs, Marcia M; Almond, Glen W; Orndorff, Paul E; Elkins, Christopher

    2011-08-01

    Haemophilus ducreyi, the etiologic agent of chancroid, has an obligate requirement for heme. Heme is acquired by H. ducreyi from its human host via TonB-dependent transporters expressed at its bacterial surface. Of 3 TonB-dependent transporters encoded in the genome of H. ducreyi, only the hemoglobin receptor, HgbA, is required to establish infection during the early stages of the experimental human model of chancroid. Active immunization with a native preparation of HgbA (nHgbA) confers complete protection in the experimental swine model of chancroid, using either Freund's or monophosphoryl lipid A as adjuvants. To determine if transfer of anti-nHgbA serum is sufficient to confer protection, a passive immunization experiment using pooled nHgbA antiserum was conducted in the experimental swine model of chancroid. Pigs receiving this pooled nHgbA antiserum were protected from a homologous, but not a heterologous, challenge. Passively transferred polyclonal antibodies elicited to nHgbA bound the surface of H. ducreyi and partially blocked hemoglobin binding by nHgbA, but were not bactericidal. Taken together, these data suggest that the humoral immune response to the HgbA vaccine is protective against an H. ducreyi infection, possibly by preventing acquisition of the essential nutrient heme.

  19. A novel vaccination strategy mediating the induction of lung-resident memory CD8 T cells confers heterosubtypic immunity against future pandemic influenza virus

    PubMed Central

    Lee, Yu-Na; Lee, Young-Tae; Kim, Min-Chul; Gewirtz, Andrew T.; Kang, Sang-Moo

    2016-01-01

    The currently used vaccine strategy to combat influenza A virus (IAV) aims to provide highly specific immunity to circulating seasonal IAV strains. However, the outbreak of 2009 influenza pandemic highlights the danger in this strategy. Here, we tested the hypothesis that universal vaccination that offers broader but weaker protection would result in cross protective T-cell responses after primary IAV infection, which would subsequently provide protective immunity against future pandemic strains. Specifically, we used tandem repeat M2e epitopes on virus-like particles (M2e5x VLP) that induced heterosubtypic immunity by eliciting antibodies to a conserved M2e epitope. M2e5x VLP was found to be superior to strain-specific current split vaccine in conferring heterosubtypic cross protection and in equipping the host with cross-protective lung-resident nucleoprotein-specific memory CD8+ T cell responses to a subsequent secondary infection with a new pandemic potential strain. Immune correlates for subsequent heterosubtypic immunity by M2e5x VLP vaccination were found to be virus-specific CD8+ T cells secreting IFN-γ and expressing lung-resident memory phenotypic markers CD69+ and CD103+ as well as M2e antibodies. Hence, vaccination with M2e5x VLP may be developable as a new strategy to combat future pandemic outbreaks. PMID:26864033

  20. Intranasal immunization with fusion protein MrpH·FimH and MPL adjuvant confers protection against urinary tract infections caused by uropathogenic Escherichia coli and Proteus mirabilis.

    PubMed

    Habibi, Mehri; Asadi Karam, Mohammad Reza; Shokrgozar, Mohammad Ali; Oloomi, Mana; Jafari, Anis; Bouzari, Saeid

    2015-04-01

    Urinary tract infections (UTIs) caused by Uropathogenic Escherichia coli (UPEC) and Proteus mirabilis are among the most common infections in the world. Currently there are no vaccines available to confer protection against UTI in humans. In this study, the immune responses and protection of FimH of UPEC with MrpH antigen of P. mirabilis in different vaccine formulations with and without MPL adjuvant were assessed. Mice intranasally immunized with the novel fusion protein MrpH·FimH induced a significant increase in IgG and IgA in serum, nasal wash, vaginal wash, and urine samples. Mice immunized with fusion MrpH·FimH also showed a significant boost in cellular immunity. Addition of MPL as the adjuvant enhanced FimH and MrpH specific humoral and cellular responses in both systemic and mucosal samples. Vaccination with MrpH·FimH alone or in combination with MPL showed the highest efficiency in clearing bladder and kidney infections in mice challenged with UPEC and P. mirabilis. These findings may indicate that the protection observed correlates with the systemic, mucosal and cellular immune responses induced by vaccination with these preparations. Our data suggest MrpH·FimH fusion protein with or without MPL as adjuvant could be potential vaccine candidates for elimination of UPEC and P. mirabilis. These data altogether are promising and these formulations are good candidates for elimination of UPEC and P. mirabilis. Copyright © 2014 Elsevier Ltd. All rights reserved.

  1. Public Access to Digital Material; A Call to Researchers: Digital Libraries Need Collaboration across Disciplines; Greenstone: Open-Source Digital Library Software; Retrieval Issues for the Colorado Digitization Project's Heritage Database; Report on the 5th European Conference on Digital Libraries, ECDL 2001; Report on the First Joint Conference on Digital Libraries.

    ERIC Educational Resources Information Center

    Kahle, Brewster; Prelinger, Rick; Jackson, Mary E.; Boyack, Kevin W.; Wylie, Brian N.; Davidson, George S.; Witten, Ian H.; Bainbridge, David; Boddie, Stefan J.; Garrison, William A.; Cunningham, Sally Jo; Borgman, Christine L.; Hessel, Heather

    2001-01-01

    These six articles discuss various issues relating to digital libraries. Highlights include public access to digital materials; intellectual property concerns; the need for collaboration across disciplines; Greenstone software for construction and presentation of digital information collections; the Colorado Digitization Project; and conferences…

  2. Public Access to Digital Material; A Call to Researchers: Digital Libraries Need Collaboration across Disciplines; Greenstone: Open-Source Digital Library Software; Retrieval Issues for the Colorado Digitization Project's Heritage Database; Report on the 5th European Conference on Digital Libraries, ECDL 2001; Report on the First Joint Conference on Digital Libraries.

    ERIC Educational Resources Information Center

    Kahle, Brewster; Prelinger, Rick; Jackson, Mary E.; Boyack, Kevin W.; Wylie, Brian N.; Davidson, George S.; Witten, Ian H.; Bainbridge, David; Boddie, Stefan J.; Garrison, William A.; Cunningham, Sally Jo; Borgman, Christine L.; Hessel, Heather

    2001-01-01

    These six articles discuss various issues relating to digital libraries. Highlights include public access to digital materials; intellectual property concerns; the need for collaboration across disciplines; Greenstone software for construction and presentation of digital information collections; the Colorado Digitization Project; and conferences…

  3. International Federation of Library Associations Annual Conference. Papers of the Special Libraries Division: Geographical and Map, Science and Technology and Social Science Libraries Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Sprudzs, Adolf; And Others

    This set of eight papers includes papers presented by participants from the United States, France, East Germany, the United Kingdom, West Germany, and the USSR: "Problems with Sources of Information in International Law and Relations: The Case of the World-Wide Treaty Jungle," by Adolf Sprudzs; "French Map Libraries and National and…

  4. International Federation of Library Associations Annual Conference. Papers of the Special Libraries Division: Geographical and Map, Science and Technology and Social Science Libraries Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Sprudzs, Adolf; And Others

    This set of eight papers includes papers presented by participants from the United States, France, East Germany, the United Kingdom, West Germany, and the USSR: "Problems with Sources of Information in International Law and Relations: The Case of the World-Wide Treaty Jungle," by Adolf Sprudzs; "French Map Libraries and National and…

  5. Identification of genes conferring arsenic resistance to Escherichia coli from an effluent treatment plant sludge metagenomic library.

    PubMed

    Chauhan, Nar Singh; Ranjan, Ravi; Purohit, Hemant J; Kalia, Vipin C; Sharma, Rakesh

    2009-01-01

    The majority of bacteria elude culture in the laboratory. A metagenomic approach provides culture-independent access to the gene pool of the whole bacterial community. A metagenomic library was constructed from an industrial effluent treatment plant sludge containing about 1.25 Gb of microbial community DNA. Two arsenic-resistant clones were selected from the metagenomic library. Clones MT3 and MT6 had eight- and 18-fold higher resistance to sodium arsenate in comparison with the parent strain, respectively. The clones also showed increased resistance to arsenite but not to antimony. Sequence analysis of the clones revealed genes encoding for putative arsenate reductases and arsenite efflux pumps. A novel arsenate resistance gene (arsN) encoding a protein with similarity to acetyltransferases was identified from clone MT6. ArsN homologues were found to be closely associated with arsenic resistance genes in many bacterial genomes. ArsN homologues were found fused to putative arsenate reductases in Methylibium petroleiphilum PM1 and Anaeromyxobacter dehalogenans 2CP-C and with a putative arsenite chaperone in Burkholderia vietnamiensis G4. ArsN alone resulted in an approximately sixfold higher resistance to sodium arsenate in wild-type Escherichia coli W3110.

  6. Report of the 2014 Cent Gardes HIV Vaccine Conference-Part 2: Cell-mediated immunity, mucosal protection, and clinical trials: Fondation Mérieux Conference Center, Veyrier du Lac, France, 5-7 October, 2014.

    PubMed

    Girard, Marc P; Picot, Valentina; Longuet, Christophe; Nabel, Gary J

    2015-08-07

    The 2014 Cent Gardes Conference took place on October 5-7, 2014, at the Fondation Mérieux Conference Center, on the shores of the Annecy Lake and aimed to review the progress and promise of HIV vaccines. The elicitation of broadly neutralizing antibodies (bNAbs), their use in passive immunization, as well as their genetic delivery (vector immunoprophylaxis) by a recombinant Adenovirus-associated virus (AAV) vector were reviewed in a preceding article [1]. Approaches to the elicitation of long-lasting T cell or mucosal immunity were also discussed and are now reviewed here. The possibility of eliciting mucosal IgAs was discussed, since it was demonstrated that transcytosis-blocking IgAs can protect monkeys against repeated vaginal challenge with a pathogenic chimeric simian and human immunodeficiency virus (SHIV). The possibility of purging the HIV reservoirs from HIV-infected persons and developing a cure of the disease was also discussed. Copyright © 2015. Published by Elsevier Ltd.. All rights reserved.

  7. National Libraries Section. General Research Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on national library services and activities, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The National Library of China in its Gradual Application of Modern Technology," a discussion by Zhu Nan and Zhu Yan (China) of microform usage and library automation; (2)…

  8. National Libraries Section. General Research Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on national library services and activities, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The National Library of China in its Gradual Application of Modern Technology," a discussion by Zhu Nan and Zhu Yan (China) of microform usage and library automation; (2)…

  9. Public Libraries Section. Libraries Serving General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on public libraries, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Role of Public Libraries in Developing Countries with Particular Reference to the Gambia" by Sally P. C. N'Jie (The Gambia); (2) "Public Libraries in the Federal Republic of Germany…

  10. Public Libraries Section. Libraries Serving General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on public libraries, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Role of Public Libraries in Developing Countries with Particular Reference to the Gambia" by Sally P. C. N'Jie (The Gambia); (2) "Public Libraries in the Federal Republic of Germany…

  11. Selection of Single Domain Antibodies from Immune Libraries Displayed on the Surface of E. coli Cells with Two β-Domains of Opposite Topologies

    PubMed Central

    Martínez-Arteaga, Rocio; Ruano-Gallego, David; Fraile, Sofía; Margolles, Yago; Teira, Xema; Gutierrez, Carlos; Bodelón, Gustavo; Fernández, Luis Ángel

    2013-01-01

    Screening of antibody (Ab) libraries by direct display on the surface of E. coli cells is hampered by the presence of the outer membrane (OM). In this work we demonstrate that the native β-domains of EhaA autotransporter and intimin, two proteins from enterohemorrhagic E. coli O157:H7 (EHEC) with opposite topologies in the OM, are effective systems for the display of immune libraries of single domain Abs (sdAbs) from camelids (nanobodies or VHH) on the surface of E. coli K-12 cells and for the selection of high affinity sdAbs using magnetic cell sorting (MACS). We analyzed the capacity of EhaA and intimin β-domains to display individual sdAbs and sdAb libraries obtained after immunization with the extracellular domain of the translocated intimin receptor from EHEC (TirMEHEC). We demonstrated that both systems displayed functional sdAbs on the surface of E. coli cells with little proteolysis and cellular toxicity, although E. coli cells displaying sdAbs with the β-domain of intimin showed higher antigen-binding capacity. Both E. coli display libraries were screened for TirMEHEC binding clones by MACS. High affinity binders were selected by both display systems, although more efficiently with the intimin β-domain. The specificity of the selected clones against TirMEHEC was demonstrated by flow cytometry of E. coli cells, along with ELISA and surface plasmon resonance with purified sdAbs. Finally, we employed the E. coli cell display systems to provide an estimation of the affinity of the selected sdAb by flow cytometry analysis under equilibrium conditions. PMID:24086454

  12. Construction of cDNA library from intestine, mesentery and coelomocyte of Apostichopus japonicus Selenka infected with Vibrio sp. and a preliminary analysis of immunity-related genes

    NASA Astrophysics Data System (ADS)

    Liu, Hongzhan; Zheng, Fengrong; Sun, Xiuqin; Cai, Yimei

    2012-06-01

    The aquaculture of sea cucumber Apostichopus japonicus (Echinodermata, Holothuroidea) has grown rapidly during recent years and has become an important sector of the marine industry in Northern China. However, with the rapid growth of the industry and the use of non-standard culture techniques, epidemic diseases of A. japonicus now pose increasing problems to the industry. To screen the genes with stress response to bacterial infection in sea cucumber at a genome wide level, we constructed a cDNA library from A. japonicus Selenka (Aspidochirotida: Stichopodidae) after infecting them with Vibrio sp. for 48 h. Total RNA was extracted from the intestine, mesentery and coelomocyte of infected sea cucumber using Trizol and mRNA was isolated by Oligotex mRNA Kits. The ligated cDNAs were transformed into DH5α, and a library of 3.24×105 clones (3.24×105 cfu mL-1) was obtained with the sizes of inserted fragments ranging from 0.8 to 2.5 kb. Sequencing the cDNA clones resulted in a total of 1106 ESTs that passed the quality control. BlastX and BlastN searches have identified 168 (31.5%) ESTs sharing significant homology with known sequences in NCBI protein or nucleotide databases. Among a panel of 25 putative immunity-related genes, serum lectin isoform, complement component 3, complement component 3-like genes were further studied by real-time PCR and they all increased more than 5 fold in response to Vibrio sp. challenge. Our library provides a valuable molecular tool for future study of invertebrate immunity against bacterial infection and our gene expression data indicates the importance of the immune system in the evolution and development of sea cucumber.

  13. Art Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on art libraries and information services for the arts, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "'I See All': Information Technology and the Universal Availability of Images" by Philip Pacey (United Kingdom); (2) "Online Databases in the Fine Arts"…

  14. Art Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on art libraries and information services for the arts, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "'I See All': Information Technology and the Universal Availability of Images" by Philip Pacey (United Kingdom); (2) "Online Databases in the Fine Arts"…

  15. The Virtual Library: Computers in Libraries Canada.

    ERIC Educational Resources Information Center

    Saunders, Laverna

    1992-01-01

    Reports on a postconference workshop on virtual libraries that was presented at the third annual Computers in Libraries Canada Conference. Highlights include networks; technological change; administrative issues, including organizational structure, staff training, strategic planning, and financial resources; a case study of the University of…

  16. Caenorhabditis elegans microRNAs of the let-7 family act in innate immune response circuits and confer robust developmental timing against pathogen stress

    PubMed Central

    Ren, Zhiji; Ambros, Victor R.

    2015-01-01

    Animals maintain their developmental robustness against natural stresses through numerous regulatory mechanisms, including the posttranscriptional regulation of gene expression by microRNAs (miRNAs). Caenorhabditis elegans miRNAs of the let-7 family (let-7-Fam) function semiredundantly to confer robust stage specificity of cell fates in the hypodermal seam cell lineages. Here, we show reciprocal regulatory interactions between let-7-Fam miRNAs and the innate immune response pathway in C. elegans. Upon infection of C. elegans larvae with the opportunistic human pathogen Pseudomonas aeruginosa, the developmental timing defects of certain let-7-Fam miRNA mutants are enhanced. This enhancement is mediated by the p38 MAPK innate immune pathway acting in opposition to let-7-Fam miRNA activity, possibly via the downstream Activating Transcription Factor-7 (ATF-7). Furthermore, let-7-Fam miRNAs appear to exert negative regulation on the worm’s resistance to P. aeruginosa infection. Our results show that the inhibition of pathogen resistance by let-7 involves downstream heterochronic genes and the p38 MAPK pathway. These findings suggest that let-7-Fam miRNAs are integrated into innate immunity gene regulatory networks, such that this family of miRNAs modulates immune responses while also ensuring robust timing of developmental events under pathogen stress. PMID:25897023

  17. Mutation of the Enterohemorrhagic Escherichia coli Core LPS Biosynthesis Enzyme RfaD Confers Hypersusceptibility to Host Intestinal Innate Immunity In vivo

    PubMed Central

    Kuo, Cheng-Ju; Chen, Jenn-Wei; Chiu, Hao-Chieh; Teng, Ching-Hao; Hsu, Tai-I; Lu, Pei-Jung; Syu, Wan-Jr; Wang, Sin-Tian; Chou, Ting-Chen; Chen, Chang-Shi

    2016-01-01

    Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important foodborne pathogen causing severe diseases in humans worldwide. Currently, there is no specific treatment available for EHEC infection and the use of conventional antibiotics is contraindicated. Therefore, identification of potential therapeutic targets and development of effective measures to control and treat EHEC infection are needed. Lipopolysaccharides (LPS) are surface glycolipids found on the outer membrane of gram-negative bacteria, including EHEC, and LPS biosynthesis has long been considered as potential anti-bacterial target. Here, we demonstrated that the EHEC rfaD gene that functions in the biosynthesis of the LPS inner core is required for the intestinal colonization and pathogenesis of EHEC in vivo. Disruption of the EHEC rfaD confers attenuated toxicity in Caenorhabditis elegans and less bacterial colonization in the intestine of C. elegans and mouse. Moreover, rfaD is also involved in the control of susceptibility of EHEC to antimicrobial peptides and host intestinal immunity. It is worth noting that rfaD mutation did not interfere with the growth kinetics when compared to the wild-type EHEC cells. Taken together, we demonstrated that mutations of the EHEC rfaD confer hypersusceptibility to host intestinal innate immunity in vivo, and suggested that targeting the RfaD or the core LPS synthesis pathway may provide alternative therapeutic regimens for EHEC infection. PMID:27570746

  18. A single immunization with a recombinant canine adenovirus expressing the rabies virus G protein confers protective immunity against rabies in mice

    SciTech Connect

    Li Jianwei; Faber, Milosz; Papaneri, Amy; Faber, Marie-Luise; McGettigan, James P.; Schnell, Matthias J.; Dietzschold, Bernhard . E-mail: bernhard.dietzschold@jefferson.edu

    2006-12-20

    Rabies vaccines based on live attenuated rabies viruses or recombinant pox viruses expressing the rabies virus (RV) glycoprotein (G) hold the greatest promise of safety and efficacy, particularly for oral immunization of wildlife. However, while these vaccines induce protective immunity in foxes, they are less effective in other animals, and safety concerns have been raised for some of these vaccines. Because canine adenovirus 2 (CAV2) is licensed for use as a live vaccine for dogs and has an excellent efficacy and safety record, we used this virus as an expression vector for the RVG. The recombinant CAV2-RV G produces virus titers similar to those produced by wild-type CAV2, indicating that the RVG gene does not affect virus replication. Comparison of RVG expressed by CAV2-RV G with that of vaccinia-RV G recombinant virus (V-RG) revealed similar amounts of RV G on the cell surface. A single intramuscular or intranasal immunization of mice with CAV2-RVG induced protective immunity in a dose-dependent manner, with no clinical signs or discomfort from the virus infection regardless of the route of administration or the amount of virus.

  19. Immunization with truncated recombinant protein SpaC of Erysipelothrix rhusiopathiae strain 715 serovar 18 confers protective immunity against challenge with various serovars.

    PubMed

    To, Ho; Someno, Shuichi; Nagai, Shinya; Koyama, Tomohiro; Nagano, Tetsuji

    2010-12-01

    Previously, we showed that surface protective antigen (Spa) proteins of Erysipelothrix rhusiopathiae can be classified into three molecular species-SpaA, SpaB, and SpaC-and that SpaC is the most broadly cross-protective antigen among the three Spa proteins. In this study, we examined the ability of the α-helical domain, which comprises the N-terminal half of SpaC, to elicit cross-protective immunity in mice and pigs. Mice actively immunized with the full-length protein (rSpaC664) or the α-helical domain (rSpaC427), but not the C-terminal domain (rSpaC253), were protected against challenge with E. rhusiopathiae serovars 1a, 2, 6, 19, and 18 expressing heterologous (SpaA or SpaB) and homologous (SpaC) Spas. The α-helical domain seemed to provide better protection than rSpaC664, although the differences did not reach statistical significance. Similarly, mice passively immunized with rabbit anti-rSpaC664 or anti-rSpaC427 sera, but not anti-rSpaC253 serum, were protected from challenge with various serovars. Pigs immunized with SpaC427 also developed specific antibodies against Spa proteins and were protected from challenge with the highly virulent heterologous E. rhusiopathiae strain Fujisawa (serovar 1a). Taken together, these results demonstrate for the first time the striking protective efficacy of the α-helical domain-mediated immunization in both mice and pigs, thereby highlighting its utility as the most promising candidate for the development of a safe and effective vaccine against erysipelas.

  20. Immunization with Truncated Recombinant Protein SpaC of Erysipelothrix rhusiopathiae Strain 715 Serovar 18 Confers Protective Immunity against Challenge with Various Serovars▿

    PubMed Central

    To, Ho; Someno, Shuichi; Nagai, Shinya; Koyama, Tomohiro; Nagano, Tetsuji

    2010-01-01

    Previously, we showed that surface protective antigen (Spa) proteins of Erysipelothrix rhusiopathiae can be classified into three molecular species—SpaA, SpaB, and SpaC—and that SpaC is the most broadly cross-protective antigen among the three Spa proteins. In this study, we examined the ability of the α-helical domain, which comprises the N-terminal half of SpaC, to elicit cross-protective immunity in mice and pigs. Mice actively immunized with the full-length protein (rSpaC664) or the α-helical domain (rSpaC427), but not the C-terminal domain (rSpaC253), were protected against challenge with E. rhusiopathiae serovars 1a, 2, 6, 19, and 18 expressing heterologous (SpaA or SpaB) and homologous (SpaC) Spas. The α-helical domain seemed to provide better protection than rSpaC664, although the differences did not reach statistical significance. Similarly, mice passively immunized with rabbit anti-rSpaC664 or anti-rSpaC427 sera, but not anti-rSpaC253 serum, were protected from challenge with various serovars. Pigs immunized with SpaC427 also developed specific antibodies against Spa proteins and were protected from challenge with the highly virulent heterologous E. rhusiopathiae strain Fujisawa (serovar 1a). Taken together, these results demonstrate for the first time the striking protective efficacy of the α-helical domain-mediated immunization in both mice and pigs, thereby highlighting its utility as the most promising candidate for the development of a safe and effective vaccine against erysipelas. PMID:20926696

  1. Ribosome display of combinatorial antibody libraries derived from mice immunized with heat-killed Xylella fastidiosa and the selection of MopB-specific single-chain antibodies.

    PubMed

    Azizi, Armaghan; Arora, Arinder; Markiv, Anatoliy; Lampe, David J; Miller, Thomas A; Kang, Angray S

    2012-04-01

    Pierce's disease is a devastating lethal disease of Vitus vinifera grapevines caused by the bacterium Xylella fastidiosa. There is no cure for Pierce's disease, and control is achieved predominantly by suppressing transmission of the glassy-winged sharpshooter insect vector. We present a simple robust approach for the generation of panels of recombinant single-chain antibodies against the surface-exposed elements of X. fastidiosa that may have potential use in diagnosis and/or disease transmission blocking studies. In vitro combinatorial antibody ribosome display libraries were assembled from immunoglobulin transcripts rescued from the spleens of mice immunized with heat-killed X. fastidiosa. The libraries were used in a single round of selection against an outer membrane protein, MopB, resulting in the isolation of a panel of recombinant antibodies. The potential use of selected anti-MopB antibodies was demonstrated by the successful application of the 4XfMopB3 antibody in an enzyme-linked immunosorbent assay (ELISA), a Western blot assay, and an immunofluorescence assay (IFA). These immortalized in vitro recombinant single-chain antibody libraries generated against heat-killed X. fastidiosa are a resource for the Pierce's disease research community that may be readily accessed for the isolation of antibodies against a plethora of X. fastidiosa surface-exposed antigenic molecules.

  2. The genetic immunization with paraflagellar rod protein-2 fused to the HSP70 confers protection against late Trypanosoma cruzi infection.

    PubMed

    Morell, María; Thomas, M Carmen; Caballero, Trinidad; Alonso, Carlos; López, Manuel C

    2006-11-30

    The immunological properties of the Trypanosoma cruzi paraflagellar rod proteins (PFR2 and PFR3) administered alone as well as fused to HSP70 have been analyzed in mice in the context of genetic immunization. The immunization of mice with the DNA vectors containing the PFRs gene or PFRs-HSP70 fused genes induced high level of IgG(2a) anti-PFRs. However, only the immunization with the PFR2-HSP70 fused genes triggers in spleen cells a statistically significant enhancement of expression of IL-12 and IFN-gamma and a decrease in the percentage of cells expressing IL-4. Likewise, the PFR2-HSP70 molecule elicits a statistically significant activation of PFR2 antigen specific CTLs. Immunization with the PFR2-HSP70 chimeric gene provided a protective response against a T. cruzi experimental infection.

  3. Mosaic vaccines elicit CD8+ T cell responses in monkeys that confer immune coverage of diverse HIV strains

    SciTech Connect

    Fischer, Will; Korber, Bette

    2009-01-01

    Creation of a successful HIV vaccine will require the development of a strategy to generate cellular immunity with sufficient cross-clade breadth to deal with the extreme genetic diversity of the virus. Polyvalent mosaic immunogens derived from in silica recombination of natural strains of HIV are designed to induce cellular immune responses that maximally cover the sequence diversity of circulating virus isolates. Immunization of rhesus monkeys with plasmid DNA and recombinant vaccinia virus vaccine constructs expressing either consensus immunogens or polyvalent mosaic immunogens elicited a CD4+ T lymphocyte-biased response with comparably broad epitope-specific total T lymphocyte specificities. However, immunization with the mosaic immunogens induced HIV-specific CD8+ T lymphocyte responses with markedly greater depth and breadth. Therefore, the use of polyvalent mosaic immunogens is a promising strategy for a global vaccine for HIV.

  4. SLA at 100: Conference Preview

    ERIC Educational Resources Information Center

    Blumenstein, Lynn

    2009-01-01

    When School Library Association (SLA) convenes its annual conference in Washington, DC, June 14-17, 2009, the association will be celebrating its 100th birthday. This occasion allows for grand gestures--the SLA Salutes! Awards and Leadership Reception will be held in the Library of Congress's Great Hall. The conference also draws upon Washington…

  5. SLA at 100: Conference Preview

    ERIC Educational Resources Information Center

    Blumenstein, Lynn

    2009-01-01

    When School Library Association (SLA) convenes its annual conference in Washington, DC, June 14-17, 2009, the association will be celebrating its 100th birthday. This occasion allows for grand gestures--the SLA Salutes! Awards and Leadership Reception will be held in the Library of Congress's Great Hall. The conference also draws upon Washington…

  6. Multiple heterologous M2 extracellular domains presented on virus-like particles confer broader and stronger M2 immunity than live influenza A virus infection.

    PubMed

    Kim, Min-Chul; Lee, Jong-Seok; Kwon, Young-Man; O, Eunju; Lee, Youn-Jeong; Choi, Jun-Gu; Wang, Bao-Zhong; Compans, Richard W; Kang, Sang-Moo

    2013-09-01

    The influenza M2 ectodomain (M2e) is poorly immunogenic and has some amino acid changes among isolates from different host species. We expressed a tandem repeat construct of heterologous M2e sequences (M2e5x) derived from human, swine, and avian origin influenza A viruses on virus-like particles (M2e5x VLPs) in a membrane-anchored form. Immunization of mice with M2e5x VLPs induced protective antibodies cross-reactive to antigenically different influenza A viruses and conferred cross protection. Anti-M2e antibodies induced by heterologous M2e5x VLPs showed a wider range of cross reactivity to influenza A viruses at higher levels than those by live virus infection, homologous M2e VLPs, or M2e monoclonal antibody 14C2. Fc receptors were found to be important for mediating protection by immune sera from M2e5x VLP vaccination. The present study provides evidence that heterologous recombinant M2e5x VLPs can be more effective in inducing protective M2e immunity than natural virus infection and further supports an approach for developing an effective universal influenza vaccine.

  7. Microglial expression of the B7 family member B7 homolog 1 confers strong immune inhibition: implications for immune responses and autoimmunity in the CNS.

    PubMed

    Magnus, Tim; Schreiner, Bettina; Korn, Thomas; Jack, Carolyn; Guo, Hong; Antel, Jack; Ifergan, Igal; Chen, Lieping; Bischof, Felix; Bar-Or, Amit; Wiendl, Heinz

    2005-03-09

    Inflammation of the CNS is usually locally limited to avoid devastating consequences. Critical players involved in this immune regulatory process are the resident immune cells of the brain, the microglia. Interactions between the growing family of B7 costimulatory ligands and their receptors are increasingly recognized as important pathways for costimulation and/or inhibition of immune responses. Human and mouse microglial cells constitutively express B7 homolog 1 (B7-H1) in vitro. However, under inflammatory conditions [presence of interferon-gamma (IFN-gamma) or T-helper 1 supernatants], a significant upregulation of B7-H1 was detectable. Expression levels of B7-H1 protein on microglial cells were substantially higher compared with astrocytes or splenocytes. Coculture experiments of major histocompatibility complex class II-positive antigen-presenting cells (APC) with syngeneic T cells in the presence of antigen demonstrated the functional consequences of B7-H1 expression on T-cell activation. In the presence of a neutralizing anti-B7-H1 antibody, both the production of inflammatory cytokines (IFN-gamma and interleukin-2) and the upregulation of activation markers (inducible costimulatory signal) by T cells were markedly enhanced. Interestingly, this effect was clearly more pronounced when microglial cells were used as APC, compared with astrocytes or splenocytes. Furthermore, B7-H1 was highly upregulated during the course of myelin oligodendrocyte glycoprotein-induced and proteolipid protein-induced experimental allergic encephalomyelitis in vivo. Expression was predominantly localized to areas of strongest inflammation and could be colocalized with microglial cells/macrophages as well as T cells. Together, our data propose microglial B7-H1 as an important immune inhibitory molecule capable of downregulating T-cell activation in the CNS and thus confining immunopathological damage.

  8. Voyaging from the Past, to the Present, and into the Future: Knowing Your Heritage. Selected Papers from PIALA 2003, Pacific Islands Association of Libraries, Archives and Museums Annual Conference (13th, Pohnpei, Federated States of Micronesia, November 4-6, 2003)

    ERIC Educational Resources Information Center

    Drake, Paul B., Ed.

    2003-01-01

    This publication follows the tradition of publishing selected papers from Pacific Islands Association of Libraries, Archives and Museums (PIALA) annual conferences. This 13th annual conference was held in Pohnpei, Federated States of Micronesia, November 4-6, 2003. The volume begins with a listing of the members of the PIALA 2003 Planning &…

  9. Assessment of geometry in 2D immune systems using high accuracy laser-based bioprinting techniques (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Lauzurica, Sara; Márquez, Andrés.; Molpeceres, Carlos; Notario, Laura; Gómez-Fontela, Miguel; Lauzurica, Pilar

    2017-02-01

    The immune system is a very complex system that comprises a network of genetic and signaling pathways subtending a network of interacting cells. The location of the cells in a network, along with the gene products they interact with, rules the behavior of the immune system. Therefore, there is a great interest in understanding properly the role of a cell in such networks to increase our knowledge of the immune system response. In order to acquire a better understanding of these processes, cell printing with high spatial resolution emerges as one of the promising approaches to organize cells in two and three-dimensional patterns to enable the study the geometry influence in these interactions. In particular, laser assisted bio-printing techniques using sub-nanosecond laser sources have better characteristics for application in this field, mainly due to its higher spatial resolution, cell viability percentage and process automation. This work presents laser assisted bio-printing of antigen-presenting cells (APCs) in two-dimensional geometries, placing cellular components on a matrix previously generated on demand, permitting to test the molecular interactions between APCs and lymphocytes; as well as the generation of two-dimensional structures designed ad hoc in order to study the mechanisms of mobilization of immune system cells. The use of laser assisted bio-printing, along with APCs and lymphocytes emulate the structure of different niches of the immune system so that we can analyse functional requirement of these interaction.

  10. Mosaic vaccines elicit CD8+ T lymphocyte responses that confer enhanced immune coverage of diverse HIV strains in monkeys.

    PubMed

    Santra, Sampa; Liao, Hua-Xin; Zhang, Ruijin; Muldoon, Mark; Watson, Sydeaka; Fischer, Will; Theiler, James; Szinger, James; Balachandran, Harikrishnan; Buzby, Adam; Quinn, David; Parks, Robert J; Tsao, Chun-Yen; Carville, Angela; Mansfield, Keith G; Pavlakis, George N; Felber, Barbara K; Haynes, Barton F; Korber, Bette T; Letvin, Norman L

    2010-03-01

    An effective HIV vaccine must elicit immune responses that recognize genetically diverse viruses. It must generate CD8+ T lymphocytes that control HIV replication and CD4+ T lymphocytes that provide help for the generation and maintenance of both cellular and humoral immune responses against the virus. Creating immunogens that can elicit cellular immune responses against the genetically varied circulating isolates of HIV presents a key challenge for creating an HIV vaccine. Polyvalent mosaic immunogens derived by in silico recombination of natural strains of HIV are designed to induce cellular immune responses that recognize genetically diverse circulating virus isolates. Here we immunized rhesus monkeys by plasmid DNA prime and recombinant vaccinia virus boost with vaccine constructs expressing either consensus or polyvalent mosaic proteins. As compared to consensus immunogens, the mosaic immunogens elicited CD8+ T lymphocyte responses to more epitopes of each viral protein than did the consensus immunogens and to more variant sequences of CD8+ T lymphocyte epitopes. This increased breadth and depth of epitope recognition may contribute both to protection against infection by genetically diverse viruses and to the control of variant viruses that emerge as they mutate away from recognition by cytotoxic T lymphocytes.

  11. Nebraska Information Partnerships, A Report to Nebraskans. Nebraska Conference on Library and Information Services (Omaha, Nebraska, February 24-26, 1991).

    ERIC Educational Resources Information Center

    Nebraska Library Commission, Lincoln.

    This report begins by describing the activities of the members of the Nebraska Information Partnerships Planning Committee as they planned for the state conference, including a Delphi survey that was conducted to identify key issues and direction for discussion at the conference, and the selection of 100 delegates. Conference activities and…

  12. Nebraska Information Partnerships, A Report to Nebraskans. Nebraska Conference on Library and Information Services (Omaha, Nebraska, February 24-26, 1991).

    ERIC Educational Resources Information Center

    Nebraska Library Commission, Lincoln.

    This report begins by describing the activities of the members of the Nebraska Information Partnerships Planning Committee as they planned for the state conference, including a Delphi survey that was conducted to identify key issues and direction for discussion at the conference, and the selection of 100 delegates. Conference activities and…

  13. Single-Dose Immunization with Virus Replicon Particles Confers Rapid Robust Protection against Rift Valley Fever Virus Challenge

    PubMed Central

    Dodd, Kimberly A.; Metcalfe, Maureen G.; Nichol, Stuart T.; Albariño, César G.

    2012-01-01

    Rift Valley fever virus (RVFV) causes outbreaks of severe disease in people and livestock throughout Africa and the Arabian Peninsula. The potential for RVFV introduction outside the area of endemicity highlights the need for fast-acting, safe, and efficacious vaccines. Here, we demonstrate a robust system for the reverse genetics generation of a RVF virus replicon particle (VRPRVF) vaccine candidate. Using a mouse model, we show that VRPRVF immunization provides the optimal balance of safety and single-dose robust efficacy. VRPRVF can actively synthesize viral RNA and proteins but lacks structural glycoprotein genes, preventing spread within immunized individuals and reducing the risk of vaccine-induced pathogenicity. VRPRVF proved to be completely safe following intracranial inoculation of suckling mice, a stringent test of vaccine safety. Single-dose subcutaneous immunization with VRPRVF, although it is highly attenuated, completely protected mice against a virulent RVFV challenge dose which was 100,000-fold greater than the 50% lethal dose (LD50). Robust protection from lethal challenge was observed by 24 h postvaccination, with 100% protection induced in as little as 96 h. We show that a single subcutaneous VRPRVF immunization initiated a systemic antiviral state followed by an enhanced adaptive response. These data contrast sharply with the much-reduced survivability and immune responses observed among animals immunized with nonreplicating viral particles, indicating that replication, even if confined to the initially infected cells, contributes substantially to protective efficacy at early and late time points postimmunization. These data demonstrate that replicon vaccines successfully bridge the gap between safety and efficacy and provide insights into the kinetics of antiviral protection from RVFV infection. PMID:22345465

  14. Single-dose immunization with virus replicon particles confers rapid robust protection against Rift Valley fever virus challenge.

    PubMed

    Dodd, Kimberly A; Bird, Brian H; Metcalfe, Maureen G; Nichol, Stuart T; Albariño, César G

    2012-04-01

    Rift Valley fever virus (RVFV) causes outbreaks of severe disease in people and livestock throughout Africa and the Arabian Peninsula. The potential for RVFV introduction outside the area of endemicity highlights the need for fast-acting, safe, and efficacious vaccines. Here, we demonstrate a robust system for the reverse genetics generation of a RVF virus replicon particle (VRP(RVF)) vaccine candidate. Using a mouse model, we show that VRP(RVF) immunization provides the optimal balance of safety and single-dose robust efficacy. VRP(RVF) can actively synthesize viral RNA and proteins but lacks structural glycoprotein genes, preventing spread within immunized individuals and reducing the risk of vaccine-induced pathogenicity. VRP(RVF) proved to be completely safe following intracranial inoculation of suckling mice, a stringent test of vaccine safety. Single-dose subcutaneous immunization with VRP(RVF), although it is highly attenuated, completely protected mice against a virulent RVFV challenge dose which was 100,000-fold greater than the 50% lethal dose (LD(50)). Robust protection from lethal challenge was observed by 24 h postvaccination, with 100% protection induced in as little as 96 h. We show that a single subcutaneous VRP(RVF) immunization initiated a systemic antiviral state followed by an enhanced adaptive response. These data contrast sharply with the much-reduced survivability and immune responses observed among animals immunized with nonreplicating viral particles, indicating that replication, even if confined to the initially infected cells, contributes substantially to protective efficacy at early and late time points postimmunization. These data demonstrate that replicon vaccines successfully bridge the gap between safety and efficacy and provide insights into the kinetics of antiviral protection from RVFV infection.

  15. International Federation of Library Associations Annual Conference Papers. Education and Research Division: Editors of Library Journals and Serial Publications Sections (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Bankole, E. Bejide; And Others

    Four papers and two reports from three sections concerned with library science periodicals and serials publications in libraries include discussions of the role of library and information science journals and their coverage by indexing services as well as a description of an online system for serials, a report from the working group on newspapers,…

  16. ALA Conference 2009: Chicago Hope

    ERIC Educational Resources Information Center

    Berry, John N., III

    2009-01-01

    There is joy among those who have the funds to go to Chicago for the American Library Association (ALA) annual conference, July 9-15. Every librarian knows there is nothing better than a Chicago gathering, with the city's wonderful haunts, museums, restaurants, and fine memories of past conferences. The conference program covers nearly every…

  17. ALA Conference 2009: Chicago Hope

    ERIC Educational Resources Information Center

    Berry, John N., III

    2009-01-01

    There is joy among those who have the funds to go to Chicago for the American Library Association (ALA) annual conference, July 9-15. Every librarian knows there is nothing better than a Chicago gathering, with the city's wonderful haunts, museums, restaurants, and fine memories of past conferences. The conference program covers nearly every…

  18. Libraries, Listservs, and LITA.

    ERIC Educational Resources Information Center

    Williams, Brian

    1992-01-01

    Explains a series of 39 slides used in a presentation covering guides to libraries on the Internet computer network; other INTERNET resources, including bibliographic databases, catalogs, and online services; BITNET servers and LISTSERVS, and the MicroUser's computer conference sponsored by the Library and Information Technology Association…

  19. Combining Raman spectroscopy and digital holographic microscopy for label-free classification of human immune cells (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    McReynolds, Naomi; Cooke, Fiona G. M.; Chen, Mingzhou; Powis, Simon J.; Dholakia, Kishan

    2017-02-01

    Moving towards label-free techniques for cell identification is essential for many clinical and research applications. Raman spectroscopy and digital holographic microscopy (DHM) are both label-free, non-destructive optical techniques capable of providing complimentary information. We demonstrate a multi-modal system which may simultaneously take Raman spectra and DHM images to provide both a molecular and a morphological description of our sample. In this study we use Raman spectroscopy and DHM to discriminate between three immune cell populations CD4+ T cells, B cells, and monocytes, which together comprise key functional immune cell subsets in immune responses to invading pathogens. Various parameters that may be used to describe the phase images are also examined such as pixel value histograms or texture analysis. Using our system it is possible to consider each technique individually or in combination. Principal component analysis is used on the data set to discriminate between cell types and leave-one-out cross-validation is used to estimate the efficiency of our method. Raman spectroscopy provides specific chemical information but requires relatively long acquisition times, combining this with a faster modality such as DHM could help achieve faster throughput rates. The combination of these two complimentary optical techniques provides a wealth of information for cell characterisation which is a step towards achieving label free technology for the identification of human immune cells.

  20. International Federation of Library Associations Annual Conference. Papers of the Management and Technology Division: Conservation Section (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Sylvestre, J. G.; And Others

    Five papers in this collection are concerned with the preservation of library materials; the remaining paper addresses library building standards, emphasizing their value and identifying other reports on library standards. The conservation papers cover: (1) training for conservation in Canada, identifying institutions and associations offering…

  1. International Federation of Library Associations Annual Conference. Papers of the Management and Technology Division: Statistics Section (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Gruber, Peter; Dosunmu, J. A.

    Two papers on library statistics describe national programs to collect such statistics in a Western European country and a developing country in Africa: "Establishment and Organisation of National Library Statistics: The Nigerian Experience," by J. A. Dosunmu; and "German Library Statistics," by Peter Gruber. Dosunmu's paper…

  2. International Federation of Library Associations Annual Conference. Papers of the Management and Technology Division: Information Technology Section (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Bradler, Reinhard; And Others

    These seven papers on library management and networks focus on: (1) computerized access to archival and library materials, describing the methodological problems associated with a pilot project in the German Democratic Republic, as well as the efficiency of data bank systems; (2) present and future development of libraries and information centers…

  3. International Federation of Library Associations Annual Conference. Papers of the Management and Technology Division: Conservation Section (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Sylvestre, J. G.; And Others

    Five papers in this collection are concerned with the preservation of library materials; the remaining paper addresses library building standards, emphasizing their value and identifying other reports on library standards. The conservation papers cover: (1) training for conservation in Canada, identifying institutions and associations offering…

  4. IOLS '98. Proceedings of the National Conference on Integrated Online Library Systems (13th, New York, New York, May 13-14, 1998).

    ERIC Educational Resources Information Center

    Cibbarelli, Pamela R., Comp.; Nixon, Carol, Comp.

    Integrated Online Library Systems (IOLS) focuses exclusively on the issues of planning and managing automated systems in libraries and information centers. The theme of the IOLS '98 annual meeting is "Embrace and Extend," and 18 papers were selected for presentation that provide insight into the current technologies being "embraced" in libraries.…

  5. International Federation of Library Associations Annual Conference. Papers of the Management and Technology Division: Information Technology Section (47th, Leipzig, East Germany, August 17-22, 1981).

    ERIC Educational Resources Information Center

    Bradler, Reinhard; And Others

    These seven papers on library management and networks focus on: (1) computerized access to archival and library materials, describing the methodological problems associated with a pilot project in the German Democratic Republic, as well as the efficiency of data bank systems; (2) present and future development of libraries and information centers…

  6. Cutting edge: recombinant Listeria monocytogenes expressing a single immune-dominant peptide confers protective immunity to herpes simplex virus-1 infection.

    PubMed

    Orr, Mark T; Orgun, Nural N; Wilson, Christopher B; Way, Sing Sing

    2007-04-15

    The vast majority of the world's population is infected with HSV. Although antiviral therapy can reduce the incidence of reactivation and asymptomatic viral shedding, and limit morbidity and mortality from active disease, it cannot cure infection. Therefore, the development of an effective vaccine is an important global health priority. In this study, we demonstrate that recombinant Listeria monocytogenes (Lm) expressing the H-2K(b) glycoprotein B (gB)(498-505) peptide from HSV-1 triggers a robust CD8 T cell response to this Ag resulting in protective immunity to HSV infection. Following challenge with HSV-1, immune-competent mice primed with recombinant Lm-expressing gB(498-505) Ag were protected from HSV-induced paralysis. Protection was associated with dramatic reductions in recoverable virus, and early expansion of HSV-1-specific CD8 T cells in the regional lymph nodes. Thus, recombinant Lm-expressing Ag from HSV represents a promising new class of vaccines against HSV infection.

  7. Science and Technology Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on scientific/technical information and libraries, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "Patents as Information--An Unused Resource" by Richard D. Walker (United States); (2) "Survey of the Information Services of the Library of the German Patent…

  8. Children's Libraries Section. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on school and children's libraries which were presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Learning to Learn in the Street," a description by B. Lang (France) of "street libraries" and other institutions designed to introduce economically disadvantaged children to…

  9. Geography and Map Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on geography and map libraries and a paper on European access to biomedical databases, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "History and Current Status of the Globe Museum of the Austrian National Library" by Franz Wawrik (Austria); (2) "Earth…

  10. Public Libraries Section. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on the impact of information technology on library services and the provision of library services to ethnic and linguistic minorities which were presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "The Challenge of Electronic Technologies to the Information Society: A Call for…

  11. National Libraries Section. General Research Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on national networks and national libraries and information centers presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Networking Developments in the U.S. at the National Level," in which Warren J. Haas predicts the development of links between existing library bibliographic…

  12. Geography and Map Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on geography and map libraries and a paper on European access to biomedical databases, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "History and Current Status of the Globe Museum of the Austrian National Library" by Franz Wawrik (Austria); (2) "Earth…

  13. Microneedle delivery of an M2e-TLR5 ligand fusion protein to skin confers broadly cross-protective influenza immunity.

    PubMed

    Wang, Bao-Zhong; Gill, Harvinder S; He, Cheng; Ou, Changbo; Wang, Li; Wang, Ying-Chun; Feng, Hao; Zhang, Han; Prausnitz, Mark R; Compans, Richard W

    2014-03-28

    Influenza vaccines with broad cross-protection are urgently needed to prevent an emerging influenza pandemic. A fusion protein of the Toll-like receptor (TLR) 5-agonist domains from flagellin and multiple repeats of the conserved extracellular domain of the influenza matrix protein 2 (M2e) was constructed, purified and evaluated as such a vaccine. A painless vaccination method suitable for possible self-administration using coated microneedle arrays was investigated for skin-targeted delivery of the fusion protein in a mouse model. The results demonstrate that microneedle immunization induced strong humoral as well as mucosal antibody responses and conferred complete protection against homo- and heterosubtypic lethal virus challenges. Protective efficacy with microneedles was found to be significantly better than that seen with conventional intramuscular injection, and comparable to that observed with intranasal immunization. Because of its advantages for administration, safety and storage, microneedle delivery of M2e-flagellin fusion protein is a promising approach for an easy-to-administer universal influenza vaccine.

  14. Hairpin RNA derived from viral NIa gene confers immunity to wheat streak mosaic virus infection in transgenic wheat plants.

    PubMed

    Fahim, Muhammad; Ayala-Navarrete, Ligia; Millar, Anthony A; Larkin, Philip J

    2010-09-01

    Wheat streak mosaic virus (WSMV), vectored by Wheat curl mite, has been of great economic importance in the Great Plains of the United States and Canada. Recently, the virus has been identified in Australia, where it has spread quickly to all major wheat growing areas. The difficulties in finding adequate natural resistance in wheat prompted us to develop transgenic resistance based on RNA interference (RNAi). An RNAi construct was designed to target the nuclear inclusion protein 'a' (NIa) gene of WSMV. Wheat was stably cotransformed with two plasmids: pStargate-NIa expressing hairpin RNA (hpRNA) including WSMV sequence and pCMneoSTLS2 with the nptII selectable marker. When T(1) progeny were assayed against WSMV, ten of sixteen families showed complete resistance in transgenic segregants. The resistance was classified as immunity by four criteria: no disease symptoms were produced; ELISA readings were as in uninoculated plants; viral sequences could not be detected by RT-PCR from leaf extracts; and leaf extracts failed to give infections in susceptible plants when used in test-inoculation experiments. Southern blot hybridization analysis indicated hpRNA transgene integrated into the wheat genome. Moreover, accumulation of small RNAs derived from the hpRNA transgene sequence positively correlated with immunity. We also showed that the selectable marker gene nptII segregated independently of the hpRNA transgene in some transgenics, and therefore demonstrated that it is possible using these techniques, to produce marker-free WSMV immune transgenic plants. This is the first report of immunity in wheat to WSMV using a spliceable intron hpRNA strategy.

  15. H3K9 Trimethylation Silences Fas Expression To Confer Colon Carcinoma Immune Escape and 5-Fluorouracil Chemoresistance.

    PubMed

    Paschall, Amy V; Yang, Dafeng; Lu, Chunwan; Choi, Jeong-Hyeon; Li, Xia; Liu, Feiyan; Figueroa, Mario; Oberlies, Nicholas H; Pearce, Cedric; Bollag, Wendy B; Nayak-Kapoor, Asha; Liu, Kebin

    2015-08-15

    The Fas-FasL effector mechanism plays a key role in cancer immune surveillance by host T cells, but metastatic human colon carcinoma often uses silencing Fas expression as a mechanism of immune evasion. The molecular mechanism under FAS transcriptional silencing in human colon carcinoma is unknown. We performed genome-wide chromatin immunoprecipitation sequencing analysis and identified that the FAS promoter is enriched with H3K9me3 in metastatic human colon carcinoma cells. The H3K9me3 level in the FAS promoter region is significantly higher in metastatic than in primary cancer cells, and it is inversely correlated with Fas expression level. We discovered that verticillin A is a selective inhibitor of histone methyltransferases SUV39H1, SUV39H2, and G9a/GLP that exhibit redundant functions in H3K9 trimethylation and FAS transcriptional silencing. Genome-wide gene expression analysis identified FAS as one of the verticillin A target genes. Verticillin A treatment decreased H3K9me3 levels in the FAS promoter and restored Fas expression. Furthermore, verticillin A exhibited greater efficacy than decitabine and vorinostat in overcoming colon carcinoma resistance to FasL-induced apoptosis. Verticillin A also increased DR5 expression and overcame colon carcinoma resistance to DR5 agonist drozitumab-induced apoptosis. Interestingly, verticillin A overcame metastatic colon carcinoma resistance to 5-fluorouracil in vitro and in vivo. Using an orthotopic colon cancer mouse model, we demonstrated that tumor-infiltrating cytotoxic T lymphocytes are FasL(+) and that FasL-mediated cancer immune surveillance is essential for colon carcinoma growth control in vivo. Our findings determine that H3K9me3 of the FAS promoter is a dominant mechanism underlying FAS silencing and resultant colon carcinoma immune evasion and progression.

  16. Intradermal immunization with ovalbumin-loaded poly-epsilon-caprolactone microparticles conferred protection in ovalbumin-sensitized allergic mice.

    PubMed

    Roman, B S; Espuelas, S; Gómez, S; Gamazo, C; Sanz, M L; Ferrer, M; Irache, J M

    2007-02-01

    Although immunotherapy has been reported as the only treatment able to revert the T-helper type 2 (Th2) response, its administration has some disadvantages such as the requirement of multiple doses, possible side-effects provoked by conventional adjuvants and the risk of suffering an anaphylactic shock. For these reasons, drug-delivery systems appear to be a promising strategy due to its ability to (i) transport the allergens, (ii) protect them from degradation, (iii) decrease the number of administrations and (iv) act as immuno-adjuvants. The aim of this work was to evaluate the properties of poly-epsilon-caprolactone (PCL) microparticles as adjuvants in immunotherapy using ovalbumin (OVA) as an allergen model. For this purpose, the protection capacity of these microparticles (OVA PCL) against OVA allergy was studied in a murine model. The humoral and cellular-induced immune response generated by OVA encapsulated into PCL microparticles was studied by immunizing BALB/c mice intradermically. Also, OVA-sensitized mice were treated with OVA PCL and OVA adsorbed to aluminium hydroxide (OVA-Alum). Fifteen days after therapy, animals were challenged with OVA and different signs of anaphylactic shock were evaluated. One single shot by an intradermal route with OVA PCL resulted in a Th2-type immune response. In OVA-sensitized mice, treatment with OVA PCL elicited high OVA-specific IgG but low levels of IgE. Furthermore, OVA PCL mice group displayed lower levels of serum histamine and higher survival rate in comparison with the positive control group. The anaphylactic shock suffered by OVA PCL-treated mice was weaker than the one induced in the OVA-Alum group. Hence, the intradermal immunization with OVA PCL microparticles induced hyposensitization in OVA-allergic mice.

  17. Innate immune memory and homeostasis may be conferred through crosstalk between the TLR3 and TLR7 pathways

    PubMed Central

    Liu, Bing; Liu, Qian; Yang, Lei; Palaniappan, Sucheendra K.; Bahar, Ivet; Thiagarajan, P. S.; Ding, Jeak Ling

    2016-01-01

    Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns (PAMPs) and stimulate the innate immune response through the production of cytokines. The innate immune response depends on the timing of encountering PAMPs, suggesting a short-term “memory.” In particular, activation of TLR3 appears to prime macrophages for the subsequent activation of TLR7, which leads to synergistically increased production of cytokines. By developing a calibrated mathematical model for the kinetics of TLR3 and TLR7 pathway crosstalk and providing experimental validation, we demonstrated the involvement of the Janus-activated kinase (JAK)–signal transducer and activator of transcription (STAT) pathway in controlling the synergistic production of cytokines. Signaling through this pathway played a dual role: It mediated the synergistic production of cytokines, thus boosting the immune response, and it also maintained homeostasis to avoid an excessive inflammatory response. Thus, we propose that the JAK-STAT pathway provides a cytokine rheostat mechanism, which enables macrophages to fine-tune their responses to multiple, temporally separated infection events involving the TLR3 and TLR7 pathways. PMID:27405980

  18. CTLA-4 gene polymorphism confers susceptibility to insulin-dependent diabetes mellitus (IDDM) independently from age and from other genetic or immune disease markers. The Belgian Diabetes Registry.

    PubMed

    Van der Auwera, B J; Vandewalle, C L; Schuit, F C; Winnock, F; De Leeuw, I H; Van Imschoot, S; Lamberigts, G; Gorus, F K

    1997-10-01

    Apart from genes in the HLA complex (IDDM1) and the variable number of tandem repeats in the 5' region of the insulin gene (INS VNTR, IDDM2), several other loci have been proposed to contribute to IDDM susceptibility. Recently, linkage and association have been shown between the cytotoxic T lymphocyte-associated protein 4 (CTLA-4) gene on chromosome 2q and IDDM. In a registry-based group of 525 recent-onset IDDM patients <40 years old we investigated the possible interactions of a CTLA-4 gene A-to-G transition polymorphism with age at clinical disease onset and with the presence or absence of established genetic (HLA-DQ, INS VNTR) and immune disease markers (autoantibodies against islet cell cytoplasm (ICA); insulin (IAA); glutamate decarboxylase (GAD65-Ab); IA-2 protein tyrosine phosphatase (IA-2-Ab)) determined within the first week of insulin treatment. In new-onset IDDM patients. G-allele-containing CTLA-4 genotypes (relative risk (RR)= 1.5; 95% confidence interval (CI) = 1.2-2.0; P < 0.005) were not preferentially associated with age at clinical presentation or with the presence of other genetic (HLA-DR3 or DR4 alleles; HLA-DQA1*0301-DQB1*0302 and/or DQA1*0501-DQB1*0201 risk haplotypes; INS VNTR I/I risk genotype) or immune (ICA, IAA, IA-2-Ab, GAD65-Ab) markers of diabetes. For 151 patients, thyrogastric autoantibodies (anti-thyroid peroxidase, anti-thyroid-stimulating hormone (TSH) receptor, anti-parietal cell, anti-intrinsic factor) were determined, but association between CTLA-4 risk genotypes and markers of polyendocrine autoimmunity could not be demonstrated before or after stratification for HLA- or INS-linked risk. In conclusion, the presence of a G-containing CTLA-4 genotype confers a moderate but significant RR for IDDM that is independent of age and genetic or immune disease markers.

  19. Library Theory and Research Section. Round Table on Library History. Education and Research Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on theory and research in library history presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "The Future of the Library's Past: Library Administrative Records Preservation, Policy and Practice," a discussion by Paul Sturges (United Kingdom) of the need for library records…

  20. University Libraries and Other General Research Libraries Section. General Research Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on university and other research libraries, presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Impact of Technology on Users of Academic and Research Libraries," in which C. Lee Jones (United States) focuses on the impact of technical advances in computing and…

  1. University Libraries and Other General Research Libraries Section. General Research Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on university and other research libraries, presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Impact of Technology on Users of Academic and Research Libraries," in which C. Lee Jones (United States) focuses on the impact of technical advances in computing and…

  2. Effective Protective Immunity to Yersinia pestis Infection Conferred by DNA Vaccine Coding for Derivatives of the F1 Capsular Antigen

    PubMed Central

    Grosfeld, Haim; Cohen, Sara; Bino, Tamar; Flashner, Yehuda; Ber, Raphael; Mamroud, Emanuelle; Kronman, Chanoch; Shafferman, Avigdor; Velan, Baruch

    2003-01-01

    Three plasmids expressing derivatives of the Yersinia pestis capsular F1 antigen were evaluated for their potential as DNA vaccines. These included plasmids expressing the full-length F1, F1 devoid of its putative signal peptide (deF1), and F1 fused to the signal-bearing E3 polypeptide of Semliki Forest virus (E3/F1). Expression of these derivatives in transfected HEK293 cells revealed that deF1 is expressed in the cytosol, E3/F1 is targeted to the secretory cisternae, and the nonmodified F1 is rapidly eliminated from the cell. Intramuscular vaccination of mice with these plasmids revealed that the vector expressing deF1 was the most effective in eliciting anti-F1 antibodies. This response was not limited to specific mouse strains or to the mode of DNA administration, though gene gun-mediated vaccination was by far more effective than intramuscular needle injection. Vaccination of mice with deF1 DNA conferred protection against subcutaneous infection with the virulent Y. pestis Kimberley53 strain, even at challenge amounts as high as 4,000 50% lethal doses. Antibodies appear to play a major role in mediating this protection, as demonstrated by passive transfer of anti-deF1 DNA antiserum. Taken together, these observations indicate that a tailored genetic vaccine based on a bacterial protein can be used to confer protection against plague in mice without resorting to regimens involving the use of purified proteins. PMID:12496187

  3. Mechanism of eliciting host immunity against cancer cells treated with silica-phthalocyanine-based near infrared photoimmunotherapy (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Kobayashi, Hisataka

    2016-03-01

    Near infrared (NIR) photoimmunotherapy (PIT) is a new type of molecularly-targeted cancer photo-therapy based on conjugating a near infrared silica-phthalocyanine dye, IR700, to a monoclonal antibody (MAb) targeting cancer-specific cell-surface molecules. When exposed to NIR light, the conjugate induces a highly-selective necrotic/ immunogenic cell death (ICD) only in receptor-positive, MAb-IR700-bound cancer cells. This cell death occurs as early as 1 minute after exposure to NIR light. Meanwhile, immediately adjacent receptor-negative cells including immune cells are unharmed. Therefore, we hypothesized that NIR-PIT could efficiently elicit host immunity against treated cancer cells. Three-dimensional dynamic quantitative phase contrast microscopy and selective plane illumination microscopy of tumor cells undergoing PIT showed rapid swelling in treated cells immediately after light exposure suggesting rapid water influx into cells, followed by irreversible morphologic changes such as bleb formation, and rupture of vesicles. Furthermore, biological markers of ICD including relocation of HSP70/90 and calreticulin, and release of ATP and High Mobility Group Box 1 (HMGB1), were clearly detected immediately after NIR-PIT. When NIR-PIT was performed in a mixture of cancer cells and immature dendritic cells, maturation of immature dendritic cells was strongly induced rapidly after NIR-PIT. In summary, NIR-PIT can induce necrotic/ immunogenic cell death that promotes rapid maturation of immature dendritic cells adjacent to dying cancer cells. Therefore, NIR-PIT could efficiently initiate host immune response against NIR-PIT treated cancer cells growing in patients.

  4. The rOmp22-HpaA fusion protein confers protective immunity against helicobacter pylori in mice.

    PubMed

    Huang, Xueyong; Xu, Bianli; Duan, Guangcai; Song, Chunhua

    2013-10-01

    Helicobacter pylori (H. pylori) plays an essential role in the development of various gastroduodenal diseases; however, no vaccines preventing H. pylori infection have been available now. This study was to evaluate the protective effect of rOmp22-HpaA fusion protein against H. pylori infection in mouse model and to screen the candidate to be used in the development of an oral vaccine against H. pylori. rOmp22, rHpaA, rOmp22+rHpaA, and rOmp22-HpaA groups were used to immunize mice with mLT63 as adjuvant by intragastric route, respectively, four times at 1-week intervals. Two weeks after last immunization, all of the animals were orally challenged with H. pylori NCTC11637 and then were killed after another 2 weeks. The mice gastric tissue of all groups was separated to detect the presence of infection by urease tests, to culture H. pylori, and to observe the histological characteristics. The protective effect against H. pylori challenge in mice immunized with rOmp22-HpaA fusion protein and mLT63 adjuvant was significantly higher than PBS and mLT63 control groups (P < 0.05), but no significant difference was detected among rOmp22, rHpaA, rOmp22+rHpaA, and rOmp22-HpaA groups (P > 0.05). rOmp22-HpaA fusion protein retained immunogenicity and could be used as an antigen candidate in the development of an oral vaccine against H. pylori infection.

  5. Neutralizing activity and protective immunity to ricin toxin conferred by B subunit (RTB)-specific Fab fragments.

    PubMed

    Yermakova, Anastasiya; Mantis, Nicholas J

    2013-09-01

    SylH3 and 24B11 are murine monoclonal antibodies directed against different epitopes on ricin toxin's binding (RTB) subunit that have been shown to passively protect mice against ricin challenge. Here we report that Fab fragments of SylH3 and 24B11 neutralize ricin in a cell based assay, and in a mouse challenge model as effectively as their respective full length parental IgGs. These data demonstrate that immunity to ricin can occur independent of Fc-mediated clearance.

  6. Subcutaneous immunization with a novel immunogenic candidate (urease) confers protection against Brucella abortus and Brucella melitensis infections.

    PubMed

    Abkar, Morteza; Amani, Jafar; Sahebghadam Lotfi, Abbas; Nikbakht Brujeni, Gholamreza; Alamian, Saeed; Kamali, Mehdi

    2015-08-01

    Brucellosis is a world prevalent endemic illness that is transmitted from domestic animals to humans. Brucella spp. exploits urease for survival in the harsh conditions of stomach during the gastrointestinal infection. In this study, we examined the immune response and the protection elicited by using recombinant Brucella urease (rUrease) vaccination in BALB/c mice. The urease gene was cloned in pET28a and the resulting recombinant protein was employed as subunit vaccine. Recombinant protein was administered subcutaneously and intraperitoneally. Dosage reduction was observed with subcutaneous (SC) vaccination when compared with intraperitoneal (IP) vaccination. rUrease induced mixed Th1-Th2 immune responses with high titers of specific IgG1 and IgG2a. In lymphocyte proliferation assay, splenocytes from IP and SC-vaccinated mice displayed a strong recall proliferative response with high amounts of IL-4, IL-12 and IFN-γ production. Vaccinated mice were challenged with virulent Brucella melitensis, B. abortus and B. suis. The SC vaccination route exhibited a higher degree of protection than IP vaccination (p value ≤ 0.05). Altogether, our results indicated that rUrease could be a useful antigen candidate for the development of subunit vaccines against brucellosis.

  7. Mycobacterium indicus pranii as a booster vaccine enhances BCG induced immunity and confers higher protection in animal models of tuberculosis.

    PubMed

    Saqib, Mohd; Khatri, Rahul; Singh, Bindu; Gupta, Ananya; Kumar, Arvind; Bhaskar, Sangeeta

    2016-12-01

    BCG, the only approved vaccine protects against severe form of childhood tuberculosis but its protective efficacy wanes in adolescence. BCG has reduced the incidence of infant TB considerably in endemic areas; therefore prime-boost strategy is the most realistic measure for control of tuberculosis in near future. Mycobacterium indicus pranii (MIP) shares significant antigenic repertoire with Mtb and BCG and has been shown to impart significant protection in animal models of tuberculosis. In this study, MIP was given as a booster to BCG vaccine which enhanced the BCG mediated immune response, resulting in higher protection. MIP booster via aerosol route was found to be more effective in protection than subcutaneous route of booster immunization. Pro-inflammatory cytokines like IFN-γ, IL-12 and IL-17 were induced at higher level in infected lungs of 'BCG-MIP' group both at mRNA expression level and in secretory form when compared with 'only BCG' group. BCG-MIP groups had increased frequency of multifunctional T cells with high MFI for IFN-γ and TNF-α in Mtb infected mice. Our data demonstrate for the first time, potential application of MIP as a booster to BCG vaccine for efficient protection against tuberculosis. This could be very cost effective strategy for efficient control of tuberculosis. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. Anti-chlamydial immunity in ewes conferred by vaccination with a combination of three live chlamydia, brucella and salmonella vaccines.

    PubMed

    Souriau, A; Bosseray, N; Rodolakis, A; Lantier, F; Plommet, M

    1988-07-02

    Live attenuated vaccines against Chlamydia psittaci var ovis, Brucella melitensis and Salmonella abortus ovis have previously been shown to be compatible in mice by subcutaneous administration. Immunity against challenge with virulent chlamydia was, however, slightly decreased in associations including the B melitensis Rev 1 vaccine. The chlamydia strain 1B vaccine was administered to four- to five-month-old female lambs, either alone or in combination with the B melitensis Rev1 and the S abortus ovis Rv6 vaccines. Clinical, serological and bacteriological observations demonstrated the compatibility of the three vaccines. Control, singly and triply vaccinated ewes were challenged with a virulent strain of chlamydia during their second pregnancy, 15 months after vaccination. Five of the 12 control ewes lambed normally and 10 of them were infected, as shown by the excretion of the challenge chlamydia in genital secretions. Sixteen of the 17 ewes in the triple vaccine group lambed normally and none was infected. All 12 in the single vaccine group lambed normally and three of the 12 were infected. In spite of this unusually poor protection by the single vaccine, antichlamydial immunity was clearly not decreased by the association with the two other vaccines.

  9. Immunization with a DNA adenine methylase over-producing Yersinia pseudotuberculosis vaccine confers robust cross-protection against heterologous pathogenic serotypes.

    PubMed

    Kubicek-Sutherland, Jessica Z; Heithoff, Douglas M; Ersoy, Selvi C; Shimp, William R; Mahan, Michael J

    2014-03-14

    Yersinia pseudotuberculosis is a foodborne pathogen that can cause serious human illness. Although the source and route of transmission often remain obscure, livestock have been implicated in some cases. The diversity of yersiniae present on farms and their widespread distribution in animal and environmental reservoirs necessitates the use of broad prophylactic strategies that are efficacious against many serotypes simultaneously. Herein, immunization of mice with a modified, live attenuated Y. pseudotuberculosis vaccine that overproduces the DNA adenine methylase (Dam(OP)) conferred robust protection against virulent challenge (150-fold LD50) with homologous and heterologous serotypes that have been associated with human disease (O:1, O:1a, O:3). Further, the dam gene was shown to be essential for cell viability in all (7 of 7) Y. pseudotuberculosis strains tested. Direct selection for the inheritance of dam mutant alleles in Y. pseudotuberculosis resulted in dam strain variants that contained compensatory (second-site suppressor) mutations in genes encoding methyl-directed mismatch repair proteins (mutHLS) that are involved in suppression of the non-viable cell phenotype in all (19/19) strains tested. Such dam mutH variants exhibited a significant increase in virulence and spontaneous mutation frequency relative to that of a Dam(OP) vaccine strain. These studies indicate that Y. pseudotuberculosis Dam(OP) strains conferred potent cross-protective efficacy as well as decreased virulence and spontaneous mutation frequency relative to those that lack Dam, which have compensatory mutations in mutHLS loci. These data suggest that development of yersiniae livestock vaccines based on Dam overproduction is a viable mitigation strategy to reduce these potential foodborne contaminants.

  10. Education, Economics and TAFE Libraries. Proceedings of the National Conference of the Technical and Further Education National Section of the Australian Library and Information Association (2nd, Bundoora, Victoria, Australia, September 25-27, 1989).

    ERIC Educational Resources Information Center

    Australian Library and Information Association, Canberra. TAFE National Section.

    Seven papers address future directions that the member libraries of the Technical and Further Education (TAFE) National Section of the Australian Library and Information Association can pursue to respond to a changing environment characterized by declining public funds; the need to fulfill some of the major requirements of the government's…

  11. Comparison of the efficiency of antibody selection from semi-synthetic scFv and non-immune Fab phage display libraries against protein targets for rapid development of diagnostic immunoassays.

    PubMed

    Chan, Conrad E Z; Chan, Annie H Y; Lim, Angeline P C; Hanson, Brendon J

    2011-10-28

    Rapid development of diagnostic immunoassays against novel emerging or genetically modified pathogens in an emergency situation is dependent on the timely isolation of specific antibodies. Non-immune antibody phage display libraries are an efficient in vitro method for selecting monoclonal antibodies and hence ideal in these circumstances. Such libraries can be constructed from a variety of sources e.g. B cell cDNA or synthetically generated, and use a variety of antibody formats, typically scFv or Fab. However, antibody source and format can impact on the quality of antibodies generated and hence the effectiveness of this methodology for the timely production of antibodies. We have carried out a comparative screening of two antibody libraries, a semi-synthetic scFv library and a human-derived Fab library against the protective antigen toxin component of Bacillus anthracis and the epsilon toxin of Clostridium botulinum. We have shown that while the synthetic library produced a diverse collection of specific scFv-phage, these contained a high frequency of unnatural amber stops and glycosylation sites which limited their conversion to IgG, and also a high number which lost specificity when expressed as IgG. In contrast, these limitations were overcome by the use of a natural human library. Antibodies from both libraries could be used to develop sandwich ELISA assays with similar sensitivity. However, the ease and speed with which full-length IgG could be generated from the human-derived Fab library makes screening this type of library the preferable method for rapid antibody generation for diagnostic assay development.

  12. Marketing Your Library: Outreach, Public Relations. An Annotated Selective Bibliography on the Theme of the ARLIS/SLA Conference (New York City, New York, April 28, 1990).

    ERIC Educational Resources Information Center

    Penchansky, Mimi B., Comp.

    This annotated bibliography on public relations and libraries includes sources published primarily within the last decade, with the exception of a few key works published in earlier years. Arranged in topical sections, most of the sources originate from the library and information science literature; however, a few sources are included from the…

  13. International Conference of Directors of National Libraries on Resource Sharing in Asia and Oceania, Canberra, 1979: Papers from Australasia and Oceania.

    ERIC Educational Resources Information Center

    Ronnie, Mary; And Others

    1980-01-01

    Describes four library resource sharing projects in (1) New Zealand, (2) Papua New Guinea, (3) Australia, and (4) Fiji. Numerous shared services are discussed, including national bibliographies, publications exchanges, staff exchanges, clearing centers for duplicates, library planning, and national collections. (LLS)

  14. International Conference of Directors of National Libraries on Resource Sharing in Asia and Oceania, Canberra, 1979: Papers from Australasia and Oceania.

    ERIC Educational Resources Information Center

    Ronnie, Mary; And Others

    1980-01-01

    Describes four library resource sharing projects in (1) New Zealand, (2) Papua New Guinea, (3) Australia, and (4) Fiji. Numerous shared services are discussed, including national bibliographies, publications exchanges, staff exchanges, clearing centers for duplicates, library planning, and national collections. (LLS)

  15. Mycobacterium tuberculosis multistage antigens confer comprehensive protection against pre- and post-exposure infections by driving Th1-type T cell immunity

    PubMed Central

    Fan, Xionglin; Yu, Qi; Jing, Yukai; Wang, Weihua; Li, Li; Zhou, Zijie

    2016-01-01

    There is an urgent need for a vaccine against tuberculosis (TB) that is more effective than the current sole licensed option. However, target antigens of Mycobacterium tuberculosis with the vaccine potential remain elusive. Five immunodominant antigens with characteristic expressions at the stages of primary infection (Ag85A), the regulation of nutrition and metabolism when transferring from rapid growth to latency (PhoY2 and Rv3407), latency (Rv2626c), and reactivation (RpfB) were selected to construct the fusion polyprotein WH121, which has better immunogenicity and protection than each multistage antigen. DMT adjuvanted WH121 vaccinated C57BL/6 mice could confer persistent and significant protection against the respiratory challenge with 80 CFU of virulent M. tuberculosis H37Rv at 9 and 18 weeks after immunization, as the BCG vaccine did. Moreover, WH121/DMT could boost the BCG primed mice against post-exposure infection, and more significantly inhibit the growth of M. tuberculosis in the spleen than BCG repeat vaccination. The protection elicited by WH121/DMT is attributed to the WH121-specific Th1-type biased immune responses, characterized by increased antigen-specific IgG2a/IgG1 ratio and high levels of IFN-γ secreted by the splenocytes of vaccinated mice. In particular, high levels of IFN-γ+ TEM cells in the spleen are an effective biomarker for the vaccine-induced early protection, and the persistent protection mainly depends on the increasing IL-2+IFN-γ+CD4+ and CD8+ T cells, especially IL-2+ TCM cells. These findings demonstrate that multistage-specific antigens might be promising targets for the next generation TB vaccine, and a combination of these antigens such as WH121/DMT is required for further preclinical evaluation. PMID:27566581

  16. CRISPR/cas Loci of Type II Propionibacterium acnes Confer Immunity against Acquisition of Mobile Elements Present in Type I P. acnes

    PubMed Central

    Brüggemann, Holger; Lomholt, Hans B.; Tettelin, Hervé; Kilian, Mogens

    2012-01-01

    Propionibacterium acnes is a skin commensal that occasionally acts as an opportunistic pathogen. The population structure of this species shows three main lineages (I–III). While type I strains are mainly associated with sebaceous follicles of human skin and inflammatory acne, types II and III strains are more often associated with deep tissue infections. We investigated the occurrence and distribution of the clustered regularly interspaced short palindromic repeats (CRISPR) in P. acnes, assessed their immunological memory, and addressed the question if such a system could account for type-specific properties of the species. A collection of 108 clinical isolates covering all known phylotypes of P. acnes was screened for the existence of CRISPR/cas loci. We found that CRISPR loci are restricted to type II P. acnes strains. Sequence analyses of the CRISPR spacers revealed that the system confers immunity to P. acnes-specific phages and to two mobile genetic elements. These elements are found almost exclusively in type I P. acnes strains. Genome sequencing of a type I P. acnes isolate revealed that one element, 54 kb in size, encodes a putative secretion/tight adherence (TAD) system. Thus, CRISPR/cas loci in P. acnes recorded the exposure of type II strains to mobile genetic elements of type I strains. The CRISPR/cas locus is deleted in type I strains, which conceivably accounts for their ability to horizontally acquire fitness or virulence traits and might indicate that type I strains constitute a younger subpopulation of P. acnes. PMID:22479553

  17. Losing Libraries, Saving Libraries

    ERIC Educational Resources Information Center

    Miller, Rebecca

    2010-01-01

    This summer, as public libraries continued to get budget hit after budget hit across the country, several readers asked for a comprehensive picture of the ravages of the recession on library service. In partnership with 2010 Movers & Shakers Laura Solomon and Mandy Knapp, Ohio librarians who bought the Losing Libraries domain name,…

  18. Losing Libraries, Saving Libraries

    ERIC Educational Resources Information Center

    Miller, Rebecca

    2010-01-01

    This summer, as public libraries continued to get budget hit after budget hit across the country, several readers asked for a comprehensive picture of the ravages of the recession on library service. In partnership with 2010 Movers & Shakers Laura Solomon and Mandy Knapp, Ohio librarians who bought the Losing Libraries domain name,…

  19. Round Table Mobile Libraries Section. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Two papers on mobile libraries were presented at the 1983 International Federation of Library Associations (IFLA) conference. In "The Development, Tasks, and Organization of Mobile Libraries in West Germany," Horst Buschendorf describes the history of mobile libraries in West Germany since 1926 and notes that there are currently 150 such…

  20. Round Table Mobile Libraries Section. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Two papers on mobile libraries were presented at the 1983 International Federation of Library Associations (IFLA) conference. In "The Development, Tasks, and Organization of Mobile Libraries in West Germany," Horst Buschendorf describes the history of mobile libraries in West Germany since 1926 and notes that there are currently 150 such…

  1. Vaccination of Mice with Virulence-Associated Protein G (VapG) Antigen Confers Partial Protection against Rhodococcus equi Infection through Induced Humoral Immunity

    PubMed Central

    Trevisani, Marcel M.; Hanna, Ebert S.; Oliveira, Aline F.; Cardoso, Silvia A.; Roque-Barreira, Maria C.; Soares, Sandro G.

    2017-01-01

    Rhodococcus equi is a facultative intracellular bacterium causing severe pyogranulomatous pneumonia, ulcerative enterocolitis, and mesenteric lymphadenopathy in foals aged less than 6 months. Less frequently, this pathogen affects various other species, such as pigs, cattle, cats, and even humans. Although rhodococcosis is treated with a combination of antimicrobial agents, resistance is developed in some cases, and thus, antimicrobial susceptibility must be monitored and managed. Considering these limitations of the current therapy and unavailability of a vaccine to prevent the disease, research is particularly focused on the development of an effective vaccine against rhodococcosis. Most vaccines undergoing development utilize the virulence-associated protein (Vap) A antigen, which was identified previously as a key virulence factor of R. equi. Nevertheless, other proteins, such as VapG, present in most virulent R. equi strains, are also encoded by vap genes located on the R. equi bacterial virulence plasmid. In the present study, we evaluated the effect of VapG immunization on the survival of R. equi-challenged mice. We used attenuated Salmonella as a carrier for VapG (Salmonella-vapG+), a procedure previously adopted to develop a VapA-based vaccine. We observed that vaccination with Salmonella-vapG+ induced both an increased IFN-γ, IL-12, and TNF-α production, and a decreased bacterial burden in organs of the R. equi-challenged mice. Nevertheless, Salmonella-vapG+ vaccination protected only 50% of the mice challenged with a lethal dose of R. equi. Interestingly, we observed an increased frequency of B cells in the spleen of Salmonella-vapG+-vaccinated mice and showed that Salmonella-vapG+-vaccinated R. equi-challenged B-cell-knockout mice did not reduce the bacterial burden. Given these results, we discussed the potential role of the humoral immune response induced by Salmonella-vapG+ vaccination in conferring protection against R. equi infection, as well as

  2. Vaccination of Mice with Virulence-Associated Protein G (VapG) Antigen Confers Partial Protection against Rhodococcus equi Infection through Induced Humoral Immunity.

    PubMed

    Trevisani, Marcel M; Hanna, Ebert S; Oliveira, Aline F; Cardoso, Silvia A; Roque-Barreira, Maria C; Soares, Sandro G

    2017-01-01

    Rhodococcus equi is a facultative intracellular bacterium causing severe pyogranulomatous pneumonia, ulcerative enterocolitis, and mesenteric lymphadenopathy in foals aged less than 6 months. Less frequently, this pathogen affects various other species, such as pigs, cattle, cats, and even humans. Although rhodococcosis is treated with a combination of antimicrobial agents, resistance is developed in some cases, and thus, antimicrobial susceptibility must be monitored and managed. Considering these limitations of the current therapy and unavailability of a vaccine to prevent the disease, research is particularly focused on the development of an effective vaccine against rhodococcosis. Most vaccines undergoing development utilize the virulence-associated protein (Vap) A antigen, which was identified previously as a key virulence factor of R. equi. Nevertheless, other proteins, such as VapG, present in most virulent R. equi strains, are also encoded by vap genes located on the R. equi bacterial virulence plasmid. In the present study, we evaluated the effect of VapG immunization on the survival of R. equi-challenged mice. We used attenuated Salmonella as a carrier for VapG (Salmonella-vapG+), a procedure previously adopted to develop a VapA-based vaccine. We observed that vaccination with Salmonella-vapG+ induced both an increased IFN-γ, IL-12, and TNF-α production, and a decreased bacterial burden in organs of the R. equi-challenged mice. Nevertheless, Salmonella-vapG+ vaccination protected only 50% of the mice challenged with a lethal dose of R. equi. Interestingly, we observed an increased frequency of B cells in the spleen of Salmonella-vapG+-vaccinated mice and showed that Salmonella-vapG+-vaccinated R. equi-challenged B-cell-knockout mice did not reduce the bacterial burden. Given these results, we discussed the potential role of the humoral immune response induced by Salmonella-vapG+ vaccination in conferring protection against R. equi infection, as well as

  3. NCI, NHLBI/PBMTC First International Conference on Late Effects after Pediatric Hematopoietic Cell Transplantation: Persistent Immune Deficiency in Pediatric Transplant Survivors

    PubMed Central

    Bunin, Nancy; Small, Trudy; Szabolcs, Paul; Baker, K. Scott; Pulsipher, Michael A.; Torgerson, Troy

    2011-01-01

    Defective immune reconstitution is a major barrier to successful hematopoietic cell transplantation (HCT), and has important implications in the pediatric population. There are many factors which affect immune recovery, including stem cell source and GVHD. Complete assessment of immune recovery, including T and B lymphocyte evaluation, innate immunity and response to neoantigens, may provide insight as to infection risk and optimal time for immunizations. The increasing use of cord blood grafts requires additional study regarding early reconstitution and impact upon survival. Immunization schedules may require modification based upon stem cell source and immune reconstitution, and this is of particular importance as many children have been incompletely immunized, or not at all, prior to school entry. Additional studies are needed in children post HCT to evaluate the impact of differing stem cell sources upon immune reconstitution, infectious risks and immunization responses. PMID:22100979

  4. Social Science Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Three papers on the nonconventional literature and social science libraries were presented at the 1983 International Federation of Library Associations (IFLA) conference. In "Grey Material: A Scandinavian View," Birgitta Bergdahl (Sweden) outlines the etymology and meaning of the concept of "grey literature" (which can include…

  5. Biological & Medical Sciences Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    These two papers on the subjects of videodisc technology and medical library networking were presented at the 1982 International Federation of Library Associations (IFLA) conference. The first, "Videodisc Technology and Applications" by Earl Henderson (United States), examines the videodisc player and the premastering (five-function…

  6. Social Science Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Three papers on the nonconventional literature and social science libraries were presented at the 1983 International Federation of Library Associations (IFLA) conference. In "Grey Material: A Scandinavian View," Birgitta Bergdahl (Sweden) outlines the etymology and meaning of the concept of "grey literature" (which can include…

  7. Marketing Strategies for the Academic Library. Papers from a One Day Conference Organized by the Library Association Colleges of Further and Higher Education Group London and South East Circle (London, England, June 1985).

    ERIC Educational Resources Information Center

    Roberts, Stephen A., Ed.

    1985-01-01

    The four papers in this collection consider the attitudes, concepts, and techniques involved in marketing for non-profit organizations, and explore how librarians in higher education can use these ideas to optimize the effectiveness of their service provision in a time of economic constraint. In "Marketing the Academic Library,"…

  8. IFLA General Conference 1988. Division of Management and Technology. Section on Information Technology; Section on Statistics; Section on Library Buildings and Equipment; Section on Conservation; Round Table on Management of Library Associations; Round Table on AV Media.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 15 papers in this compilation focus on library nagement and technology, including information technology, statistics, buildings and equipment, and conservation: (1) "Information Control: OSI (Open Systems Interconnection) and Networking Strategies" (Neil McClean, United Kingdom); (2) "OSI in Australia: Potential, Planning,…

  9. Virtual Libraries: Virtual Communities. Abstracts, Fulltext Documents and PowerPoint Presentations of Papers and Demos Given at the International Association of Technological University Libraries (IATUL) Conference (Brisbane, Queensland, Australia, July 3-7, 2000).

    ERIC Educational Resources Information Center

    International Association of Technological Univ. Libraries, Gothenburg (Sweden).

    This proceedings of the International Association of Technological University Libraries (IATUL) contains the opening address by IATUL president Nancy Fjallbrant and the full text of the following papers: "Building Info-Skills by Degrees: Embedding Information Literacy in University Study" (Wendy Abbott and Deborah Peach); "UQ…

  10. Virtual Libraries: Virtual Communities. Abstracts, Fulltext Documents and PowerPoint Presentations of Papers and Demos Given at the International Association of Technological University Libraries (IATUL) Conference (Brisbane, Queensland, Australia, July 3-7, 2000).

    ERIC Educational Resources Information Center

    International Association of Technological Univ. Libraries, Gothenburg (Sweden).

    This proceedings of the International Association of Technological University Libraries (IATUL) contains the opening address by IATUL president Nancy Fjallbrant and the full text of the following papers: "Building Info-Skills by Degrees: Embedding Information Literacy in University Study" (Wendy Abbott and Deborah Peach); "UQ…

  11. The Power of Information: Transforming the World. Professional Papers from the Annual Conference of the Special Libraries Association (86th, Montreal, Quebec, Canada, June 10-15, 1995).

    ERIC Educational Resources Information Center

    Special Libraries Association, Washington, DC.

    These papers are intended to relate to a number of issues suggested by the overall conference theme of the power of information and how it is transforming the world. Most of the 11 papers cover a number of related issues--managing information, technological advances and their impacts, and new roles for librarians. Titles include: "The…

  12. Online Catalog Screen Displays. A Series of Discussions. Report of a Conference Sponsored by the Council on Library Resources (Austin, Texas, March 10-13, 1985).

    ERIC Educational Resources Information Center

    Williams, Joan Frye, Ed.

    Papers presented and summaries of discussions at a 3-day conference which focused on screen displays for online catalogs are included in this report. Papers presented were: (1) "Suggested Guidelines for Screen Layouts and Design of Online Catalogs" (Joseph R. Matthews); (2) "Displays in Database Search Systems" (Fran Spigai);…

  13. Navigating the Information Ocean: Charting the Course. Abstracts from the Academic Library Association of Ohio Annual Conference (Columbus, Ohio, November 4, 1994).

    ERIC Educational Resources Information Center

    Academic Library Association of Ohio.

    Abstracts of 14 papers presented at the conference are provided here. Titles are: "Electronic Information Terraforming: Designing and Implementing a Front-end System Using World-Wide Web Technology" (Abbie Basile; And Others); "Characteristics of Generation X and Implications for Reference and Instructional Services" (Catherine…

  14. Digital Debates. WebWise Conference on Libraries and Museums in the Digital World Proceedings (10th, Capitol Hill, Washington, D.C, February 25-27, 2009)

    ERIC Educational Resources Information Center

    Zorich, Diane

    2010-01-01

    Debates typically invoke an image of individuals arguing over the merits of opposing viewpoints. However, the term has a softer, more deliberative sense that connotes reflection, discussion, and consideration. The 2009 WebWise conference, titled "Digital Debates," was conducted in this spirit, with panelists and attendees engaged in…

  15. The Power of Information: Transforming the World. Professional Papers from the Annual Conference of the Special Libraries Association (86th, Montreal, Quebec, Canada, June 10-15, 1995).

    ERIC Educational Resources Information Center

    Special Libraries Association, Washington, DC.

    These papers are intended to relate to a number of issues suggested by the overall conference theme of the power of information and how it is transforming the world. Most of the 11 papers cover a number of related issues--managing information, technological advances and their impacts, and new roles for librarians. Titles include: "The…

  16. Crossing Borders: New Territories in the 90's. Contributed Papers Presented at the Arizona State Library Association Conference (Tucson, Arizona, November 1989).

    ERIC Educational Resources Information Center

    Hammond, Carol, Ed.

    Twelve reports comprise this collection from the 1989 Arizona State Association Conference: (1) "CD ROMs Teach Online Searching" (Susan Awe); (2) "Collection Development as Intellectual Activity: An East Asian Specialist's View" (Ju-yen Teng); (3) "Computer-Assisted Reference Service in Map Librarianship: Electronic Access to Cartographic…

  17. IFLA General Conference 1988. Opening and Plenary Sessions. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Focusing on the conference theme "Living Together: People, Libraries, Information," the opening speech of the 54th International Federation of Library Associations (IFLA) General Conference was presented by association president Hans-Peter Geh. This paper reflects on: (1) the significance of holding the conference in Australia; (2) the role of…

  18. Interconnections: An Information Technology Conference Series. Final Report.

    ERIC Educational Resources Information Center

    Pacey, Brenda

    Lincoln Trail Libraries System, with cooperation from Rolling Prairie Library System, was awarded a LSCA (Library Services and Construction Act) Title III grant to coordinate a series of four statewide "Interconnections" information technology conferences. These conferences were planned cooperatively by and aimed at attendees…

  19. Library and Information Service Needs of the Nation. Proceedings of a Conference on the Needs of Occupational, Ethnic, and other Groups in the United States.

    ERIC Educational Resources Information Center

    Caudra, Carlos A., Ed.; Bates, Marcia J., Ed.

    In 1972, the National Commission on Libraries and Information Science (NCLIS) identified experts to study and report on the information needs of selected groups within the United States population: six occupational and professional groups, various age groups, women, the handicapped, the isolated, and ethnic groups--mainly the Mexican American…

  20. IFLA Council and General Conference: Conference Programme and Proceedings (65th, Bangkok, Thailand, August 20-28, 1999).

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This proceedings of the 1999 IFLA (International Federation of Library Associations and Institutions) annual conference contains: information on the current, future, and past conferences; a message from H.R.H. Princess Maha Chakri Sirindhorn, the patron of the Thai Library Association; a conference program; an alphabetical list of authors; and 112…

  1. 3rd Annual PIALA Conference Saipan--Collecting, Preserving & Sharing Information in Micronesia. Conference Proceedings. October 13-15, 1993.

    ERIC Educational Resources Information Center

    Edmundson, Margaret, Ed.

    1993-01-01

    This PIALA 1993 Proceedings contains many of the papers presented at the 3rd annual conference of the Pacific Islands Association of Libraries and Archives. This publication is the first time papers from this Micronesian regional library and archives conference have ever been published. The conference addressed various topics of interest to…

  2. 3rd Annual PIALA Conference Saipan--Collecting, Preserving & Sharing Information in Micronesia. Conference Proceedings. October 13-15, 1993.

    ERIC Educational Resources Information Center

    Edmundson, Margaret, Ed.

    1993-01-01

    This PIALA 1993 Proceedings contains many of the papers presented at the 3rd annual conference of the Pacific Islands Association of Libraries and Archives. This publication is the first time papers from this Micronesian regional library and archives conference have ever been published. The conference addressed various topics of interest to…

  3. Library Instruction 2.0

    ERIC Educational Resources Information Center

    Bridgewater, Rachel; Deitering, Anne-Marie; Munro, Karen

    2009-01-01

    At the 2008 ALA Annual Conference, a wonderful Association of College and Research Libraries (ACRL) preconference was presented entitled "Library Instruction 2.0: Building Your Online Instruction Toolkit". The presentation was enlightening and provided numerous and valuable recommendations for Web 2.0 sites that can facilitate and enliven library…

  4. Library Instruction 2.0

    ERIC Educational Resources Information Center

    Bridgewater, Rachel; Deitering, Anne-Marie; Munro, Karen

    2009-01-01

    At the 2008 ALA Annual Conference, a wonderful Association of College and Research Libraries (ACRL) preconference was presented entitled "Library Instruction 2.0: Building Your Online Instruction Toolkit". The presentation was enlightening and provided numerous and valuable recommendations for Web 2.0 sites that can facilitate and enliven library…

  5. Macintoshed Libraries 2.0.

    ERIC Educational Resources Information Center

    Vaccaro, Bill, Ed.; Valauskas, Edward J., Ed.

    This annual collection contains 18 papers about the use of Macintosh computers in libraries. Papers include: "The Macintosh as a Wayfinding Tool for Professional Conferences: The LITA '88 HyperCard Stack" (Ann F. Bevilacqua); "Enhancing Library Services with the Macintosh" (Naomi C. Broering); "Scanning Technologies in…

  6. Concept, Challenge, Conundrum: From Library Skills to Information Literacy. Proceedings of the National Information Literacy Conference Conducted by the University of South Australia Library and the Australian Library and Information Association Information Literacy Special Interest Group (4th, Adelaide, Australia, December 3-5, 1999).

    ERIC Educational Resources Information Center

    Booker, Di, Ed.

    These proceedings from the fourth National Information Literacy Conference (Decemeber 1999) include the keynote addresses and the papers presented in workshop sessions throughout the conference. Acknowledgements by Irene Doskatsch, conference convener, that precedes the introductory piece by Alan Bundy, "Journey Without End..." is…

  7. Oregonians and Their Library and Information Services. A Progress Report.

    ERIC Educational Resources Information Center

    Thorne, Maggie, Comp.

    Compiled in response to questions from Oregon citizens about library and information services in their state, this report contains: (1) a general description of the Governor's Conference on Library and Information Services held in June 1978; (2) a delegate's viewpoint on the White House Conference on Library and Information Services (WHCLIS); (3)…

  8. Oregonians and Their Library and Information Services. A Progress Report.

    ERIC Educational Resources Information Center

    Thorne, Maggie, Comp.

    Compiled in response to questions from Oregon citizens about library and information services in their state, this report contains: (1) a general description of the Governor's Conference on Library and Information Services held in June 1978; (2) a delegate's viewpoint on the White House Conference on Library and Information Services (WHCLIS); (3)…

  9. PIALA 2000: Libraries and Archives--Where Information and Language Literacy Begin [and] Engaged Readers and Writers in Multicultural Island Communities. Selected Papers from the 10th Pacific Islands Association of Libraries and Archives Conference Joint with the 13th Annual Regional Language Arts Conference (Tumon, Guam, November 9-11, 2000)

    ERIC Educational Resources Information Center

    Cohen, Arlene, Ed.; Quan, Clarisa G., Ed.

    2004-01-01

    This proceedings combines presentations from the jointly held 10th Annual PIALA 2000 Conference and the 13th Annual Regional Language Arts Conference. The volume begins with the welcoming remarks of Mary L. Silk, Christine Ku Scott-Smith, Antonio R. Umpingco, Delia Munoz Rosal, Lawrence Kasperbauer, Rosie Tainatongo, Richard S. Tom, Mary L.…

  10. Libraries and Automation.

    ERIC Educational Resources Information Center

    Markuson, Barbara Evans, Ed.

    The arrangement of this publication follows, as nearly as possible, the actual conference program. The seven sections represent the topics selected by the planning committee as areas of concentration. Each section includes a state-of-the-art paper and related talks and discussion. The topics covered are: the library of the future, file…

  11. School Learners & Libraries.

    ERIC Educational Resources Information Center

    Karlsson, Jenni, Ed.

    This document presents papers from a conference held in November 1995 in Durban, KwaZulu-Natal, convened by the Education Policy Unit (EPU) of the University of Natal as part of research concerning the provision of library-based resources for school learners. Following an introduction are two chapters: "Identifying the Inherited Problems in…

  12. Measles-mumps-rubella vaccine and autistic spectrum disorder: report from the New Challenges in Childhood Immunizations Conference convened in Oak Brook, Illinois, June 12-13, 2000.

    PubMed

    Halsey, N A; Hyman, S L

    2001-05-01

    Parents and physicians are understandably concerned about the causes and treatment of autism, a devastating disease that affects the entire family. Although much has been learned about autism, there are many gaps in our knowledge about what causes the disorder and how it can be prevented. Autistic symptoms occur along a spectrum, often referred to as autistic spectrum disorder (ASD). Concern has been raised about a possible association between measles-mumps-rubella (MMR) vaccine and inflammatory bowel disease (IBD) and ASD, especially autism with regression. Also, increased requests for educational services related to ASD have raised concerns about possible increases in the incidence of ASD. On June 12-13, 2000, the American Academy of Pediatrics (AAP) convened a conference titled "New Challenges in Childhood Immunizations" in Oak Brook, Illinois. At this conference, parents, practitioners, and scientists presented information and research on MMR vaccine and ASD. Attendees included representatives from select AAP committees and sections as well as federal and other organizations that address related issues. The multidisciplinary panel of experts reviewed data on what is known about the pathogenesis, epidemiology, and genetics of ASD and the available data on hypothesized associations with IBD, measles, and MMR vaccine. Supplemental information was requested from authors who have proposed the hypotheses and other experts in relevant areas. Autism is a complex disorder of uncertain and probably multiple etiologies. Genetic predisposition to ASD may involve as many as 10 genes. Many experts believe that the abnormal brain development in autism occurs before 30 weeks' gestation in most instances. In utero rubella is a known cause of autism. Animal model data support the biologic plausibility that exposure to yet unrecognized infectious or other environmental agents could cause ASD. Several factors may contribute to apparent increases in incidence of ASD in recent years

  13. Perspectives [on Library Service Developments] 1991.

    ERIC Educational Resources Information Center

    Bookmark, 1991

    1991-01-01

    This theme issue of "The Bookmark" provides 1991 perspectives on several library service developments, including papers by three New Yorkers who played an important role in the White House Conference on Library and Information Services: Thomas Sobol, Commissioner of Education; Robert Wedgeworth, Dean of the School of Library Service at…

  14. Libraries and Democracy: Information for All.

    ERIC Educational Resources Information Center

    Marston, Betsy

    1991-01-01

    Discusses libraries and democracy, one of three major themes for the 1991 White House Conference on Library and Information Services. The roles of newspapers and journalists are considered and the censorship of textbooks, library materials, art, and movies is discussed. (LRW)

  15. Creating Library Spaces: Libraries 2040.

    ERIC Educational Resources Information Center

    Bruijnzeels, Rob

    This paper suggests that by 2004, the traditional public libraries will have ceased to exist and new, attractive future libraries will have taken their place. The Libraries 2040 project of the Netherlands is initiating seven different libraries of the future. The Brabant library is the "ultimate library of the future" for the Dutch…

  16. IFLA Council and General Conference: Conference Proceedings (66th, Jerusalem, Israel, August 13-18, 2000).

    ERIC Educational Resources Information Center

    International Federation of Library Associations and Institutions, The Hague (Netherlands).

    This proceedings of the 2000 IFLA (International Federation of Library Associations and Institutions) annual conference contains an alphabetical list of authors and titles, a conference evaluation form, frequently asked questions about the IFLA conference, the convening notice and agenda, IFLA Council resolutions proposed by the Executive Board,…

  17. 75 FR 45647 - National Library of Medicine; Notice of Closed Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-08-03

    ... HUMAN SERVICES National Institutes of Health National Library of Medicine; Notice of Closed Meeting... Committee: National Library of Medicine Special Emphasis Panel, Conflicted Applications. Date: September 22... Library of Medicine, 6705 Rockledge Drive, Suite 301, Bethesda, MD 20817. (Telephone Conference...

  18. 7 CFR 987.80 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE DOMESTIC DATES PRODUCED OR PACKED... of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of...

  19. 7 CFR 987.80 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE DOMESTIC DATES PRODUCED OR PACKED... of immunities. The benefits, privileges, and immunities conferred upon any person by virtue of...

  20. 7 CFR 929.72 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE CRANBERRIES GROWN IN STATES OF... Duration of immunities. The benefits, privileges, and immunities conferred upon any person by virtue...

  1. 7 CFR 985.69 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE MARKETING ORDER REGULATING THE....69 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  2. 7 CFR 906.58 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE ORANGES AND GRAPEFRUIT GROWN IN... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  3. 7 CFR 905.85 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE ORANGES, GRAPEFRUIT, TANGERINES... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part...

  4. 7 CFR 985.69 - Duration of immunities.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE MARKETING ORDER REGULATING THE....69 Duration of immunities. The benefits, privileges, and immunities conferred upon any person...

  5. 7 CFR 905.85 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE ORANGES, GRAPEFRUIT, TANGERINES... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this part...

  6. 7 CFR 906.58 - Duration of immunities.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE ORANGES AND GRAPEFRUIT GROWN IN... immunities. The benefits, privileges, and immunities conferred upon any person by virtue of this...

  7. 7th EFIS Tatra Immunology Conference. Molecular determinants of T-cell immunity. 24-28 June 2006, High Tatra Mountains, Slovakia.

    PubMed

    Leishman, Andrew J

    2006-10-01

    This meeting was hosted by the European Federation of Immunological Societies celebrating its 7th meeting in the High Tatra Mountains of Slovakia on 24-28 June 2006. Entitled molecular determinants of T-cell immunity, the meeting covered a wide range of novel methods to regulate an unwanted immune response in autoimmunity and boost the immune system to combat viral infection and cancer.

  8. Isolation of a nanomolar scFv inhibiting the endopeptidase activity of botulinum toxin A, by single-round panning of an immune phage-displayed library of macaque origin

    PubMed Central

    2011-01-01

    Background Botulinum neurotoxin A (BoNT/A), mainly represented by subtype A1, is the most toxic substance known. It causes naturally-occurring food poisoning, and is among the biological agents at the highest risk of being weaponized. Several antibodies neutralizing BoNT/A by targeting its heavy chain (BoNT/A-H) have been isolated in the past. For the first time however, an IgG (4LCA) recently isolated by hybridoma technology and targeting the BoNT/A light chain (BoNT/A-L), was shown to inhibit BoNT/A endopeptidase activity and protect in vivo against BoNT/A. In the present study, a phage-displayed library was constructed from a macaque (Macaca fascicularis) hyper-immunized with BoNTA/L in order to isolate scFvs inhibiting BoNT/A endopeptidase activity for clinical use. Results Diversity of the scFvs constituting the library was limited due to the frequent presence, within the genes intended to be part of the library, of restriction sites utilized for its construction. After screening with several rounds of increasing stringency, as is usual with phage technology, the library got overwhelmed by phagemids encoding incomplete scFvs. The screening was successfully re-performed with a single round of high stringency. In particular, one of the isolated scFvs, 2H8, bound BoNT/A1 with a 3.3 nM affinity and effectively inhibited BoNT/A1 endopeptidase activity. The sequence encoding 2H8 was 88% identical to human germline genes and its average G-score was -0.72, quantifying the high human-like quality of 2H8. Conclusions The presence of restrictions sites within many of the sequences that were to be part of the library did not prevent the isolation of an scFv, 2H8, by an adapted panning strategy. ScFv 2H8 inhibited toxin endopeptidase activity in vitro and possessed human-like quality required for clinical development. More generally, the construction and screening of phage-displayed libraries built from hyper-immunized non-human primates is an efficient solution to isolate

  9. Library Buildings and Equipment Section. Management and Technology Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library buildings and equipment, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Impact of Technology on Library Buildings," in which Rolf Fuhlrott (West Germany) discusses construction technology (types of building materials and library building design),…

  10. Immunity to Sand Fly Salivary Protein LJM11 Modulates Host Response to Vector-Transmitted Leishmania Conferring Ulcer-Free Protection

    PubMed Central

    Gomes, Regis; Oliveira, Fabiano; Teixeira, Clarissa; Meneses, Claudio; Gilmore, Dana C; Elnaiem, Dia-Eldin; Kamhawi, Shaden; Valenzuela, Jesus G

    2012-01-01

    Leishmania vaccines that protect against needle challenge fail against the potency of a Leishmania-infected sand fly transmission. Here, we demonstrate that intradermal immunization of mice with 500 ng of the sand fly salivary recombinant protein LJM11 (rLJM11) from Lutzomyia longipalpis, in the absence of adjuvant, induces long-lasting immunity that results in ulcer-free protection against Leishmania major delivered by vector bites. This protection is antibody independent and abrogated by depletion of CD4+ T cells. Two weeks after challenge, early induction of IFN-γ specifically to rLJM11 correlates to diminished parasite replication in protected animals. At this time point, Leishmania-specific induction of IFN-γ in these mice is low in comparison with its high level in non-protected controls. We hypothesize that early control of parasites in a T-cell helper type 1 environment induced by immunity to LJM11 permits the slow development of Leishmania-specific immunity in the absence of open ulcers. Leishmania-specific immunity observed 5 weeks after infection in rLJM11-immunized mice shows a twofold increase over controls in the percentage of IFN-γ-producing CD4+ T cells. We propose LJM11 as an immunomodulator that drives an efficient and controlled protective immune response to a sand fly–transmitted Leishmania somewhat mimicking “leishmanization”-induced protective immunity but without its associated lesions. PMID:22739793

  11. Genetic Immunization with CDR3-Based Fusion Vaccine Confers Protection and Long-Term Tumor-Free Survival in a Mouse Model of Lymphoma

    PubMed Central

    Iurescia, Sandra; Fioretti, Daniela; Pierimarchi, Pasquale; Signori, Emanuela; Zonfrillo, Manuela; Tonon, Giancarlo; Fazio, Vito M.; Rinaldi, Monica

    2010-01-01

    Therapeutic vaccination against idiotype is a promising strategy for immunotherapy of B-cell malignancies. We have previously shown that CDR3-based DNA immunization can induce immune response against lymphoma and explored this strategy to provide protection in a murine B-cell lymphoma model. Here we performed vaccination employing as immunogen a naked DNA fusion product. The DNA vaccine was generated following fusion of a sequence derived from tetanus toxin fragment C to the VHCDR3109−116 epitope. Induction of tumor-specific immunity as well as ability to inhibit growth of the aggressive 38C13 lymphoma and to prolong survival of vaccinated mice has been tested. We determined that DNA fusion vaccine induced immune response, elicited a strong protective antitumor immunity, and ensured almost complete long-term tumor-free survival of vaccinated mice. Our results show that CDR3-based DNA fusion vaccines hold promise for vaccination against lymphoma. PMID:20445751

  12. The High-Tech and the Beautiful: Library Buildings, Digital Libraries, and the Future.

    ERIC Educational Resources Information Center

    Gilbert, Ellen D.

    2000-01-01

    Describes the new facilities at the New York Public Library, the British Library, and the National Library of France, including reference service, delivery time for books, and general ambience. Also reports on a conference that addressed issues related to scholarly communication and digital technology in the academic community. (LRW)

  13. The High-Tech and the Beautiful: Library Buildings, Digital Libraries, and the Future.

    ERIC Educational Resources Information Center

    Gilbert, Ellen D.

    2000-01-01

    Describes the new facilities at the New York Public Library, the British Library, and the National Library of France, including reference service, delivery time for books, and general ambience. Also reports on a conference that addressed issues related to scholarly communication and digital technology in the academic community. (LRW)

  14. Libraries for the Blind Section. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library services and developments in reading materials for the blind, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "Reading Programs for the Blind and Physically Handicapped in Public Libraries of the Polish People's Republic" by Franciszek Czajkowski…

  15. IFLA General Conference, 1990. Division of Education and Research: Section of Education and Training; Section of Library Theory and Research; Library History, Round Table; Research in Reading, Round Table; Editors of Library Journals, Round Table; and Continuing Professional Education, Round Table; Joint Workshop Papers. Booklet 7.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 21 papers in this collection were presented at the meetings of two sections and three round tables of the Division of Education and Research: (1) "Library and Information Services in Culturally Diverse Communities" (Cheryl Metoyer-Duran); (2) "Library Education in a Pluralistic Society" (Shmuel Sever); (3) "Each One an Individual:…

  16. IFLA General Conference, 1990. Division of Education and Research: Section of Education and Training; Section of Library Theory and Research; Library History, Round Table; Research in Reading, Round Table; Editors of Library Journals, Round Table; and Continuing Professional Education, Round Table; Joint Workshop Papers. Booklet 7.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    The 21 papers in this collection were presented at the meetings of two sections and three round tables of the Division of Education and Research: (1) "Library and Information Services in Culturally Diverse Communities" (Cheryl Metoyer-Duran); (2) "Library Education in a Pluralistic Society" (Shmuel Sever); (3) "Each One an Individual:…

  17. Library Computing.

    ERIC Educational Resources Information Center

    Library Journal, 1985

    1985-01-01

    This special supplement to "Library Journal" and "School Library Journal" includes articles on technological dependency, promise of computers for reluctant readers, copyright and database downloading, access to neighborhood of Mister Rogers, library acquisitions, circulating personal computers, "microcomputeritis,"…

  18. Impact of preexisting adenovirus vector immunity on immunogenicity and protection conferred with an adenovirus-based H5N1 influenza vaccine.

    PubMed

    Pandey, Aseem; Singh, Neetu; Vemula, Sai V; Couëtil, Laurent; Katz, Jacqueline M; Donis, Ruben; Sambhara, Suryaprakash; Mittal, Suresh K

    2012-01-01

    The prevalence of preexisting immunity to adenoviruses in the majority of the human population might adversely impact the development of adaptive immune responses against adenovirus vector-based vaccines. To address this issue, we primed BALB/c mice either intranasally (i.n.) or intramuscularly (i.m.) with varying doses of wild type (WT) human adenovirus subtype 5 (HAd5). Following the development of immunity against HAd5, we immunized animals via the i.n. or i.m. route of inoculation with a HAd vector (HAd-HA-NP) expressing the hemagglutinin (HA) and nucleoprotein (NP) of A/Vietnam/1203/04 (H5N1) influenza virus. The immunogenicity and protection results suggest that low levels of vector immunity (<520 virus-neutralization titer) induced by priming mice with up to 10(7) plaque forming units (p.f.u.) of HAd-WT did not adversely impact the protective efficacy of the vaccine. Furthermore, high levels of vector immunity (approximately 1500 virus-neutralization titer) induced by priming mice with 10(8) p.f.u. of HAd-WT were overcome by either increasing the vaccine dose or using alternate routes of vaccination. A further increase in the priming dose to 10(9) p.f.u. allowed only partial protection. These results suggest possible strategies to overcome the variable levels of human immunity against adenoviruses, leading to better utilization of HAd vector-based vaccines.

  19. A DNA Vaccine Encoding the Enterohemorragic Escherichia coli Shiga-Like Toxin 2 A2 and B Subunits Confers Protective Immunity to Shiga Toxin Challenge in the Murine Model▿

    PubMed Central

    Bentancor, Leticia V.; Bilen, Marcos; Brando, Romina J. Fernández; Ramos, María Victoria; Ferreira, Luis C. S.; Ghiringhelli, Pablo D.; Palermo, Marina S.

    2009-01-01

    Production of verocytotoxin or Shiga-like toxin (Stx), particularly Stx2, is the basis of hemolytic uremic syndrome, a frequently lethal outcome for subjects infected with Stx2-producing enterohemorrhagic Escherichia coli (EHEC) strains. The toxin is formed by a single A subunit, which promotes protein synthesis inhibition in eukaryotic cells, and five B subunits, which bind to globotriaosylceramide at the surface of host cells. Host enzymes cleave the A subunit into the A1 peptide, endowed with N-glycosidase activity to the 28S rRNA, and the A2 peptide, which confers stability to the B pentamer. We report the construction of a DNA vaccine (pStx2ΔAB) that expresses a nontoxic Stx2 mutated form consisting of the last 32 amino acids of the A2 sequence and the complete B subunit as two nonfused polypeptides. Immunization trials carried out with the DNA vaccine in BALB/c mice, alone or in combination with another DNA vaccine encoding granulocyte-macrophage colony-stimulating factor, resulted in systemic Stx-specific antibody responses targeting both A and B subunits of the native Stx2. Moreover, anti-Stx2 antibodies raised in mice immunized with pStx2ΔAB showed toxin neutralization activity in vitro and, more importantly, conferred partial protection to Stx2 challenge in vivo. The present vector represents the second DNA vaccine so far reported to induce protective immunity to Stx2 and may contribute, either alone or in combination with other procedures, to the development of prophylactic or therapeutic interventions aiming to ameliorate EHEC infection-associated sequelae. PMID:19176691

  20. Preparing for the 21st Century. Proceedings of the Mountain Plains Library Association Academic Library Section Research Forum (Silver Creek, Colorado, October 11-15, 1986).

    ERIC Educational Resources Information Center

    Hatfield, V. Sue, Ed.

    The following papers were presented at a joint conference of the Colorado Library Association and the Mountain Plains Library Association (MPLA) in October 1986: (1) "Development of a Strategic Plan for the Harold B. Lee Library--A Model for Public Service" (Larry Ostler); (2) "The Library Committee in MPLA Libraries" (Ronald…

  1. Preparing for the 21st Century. Proceedings of the Mountain Plains Library Association Academic Library Section Research Forum (Silver Creek, Colorado, October 11-15, 1986).

    ERIC Educational Resources Information Center

    Hatfield, V. Sue, Ed.

    The following papers were presented at a joint conference of the Colorado Library Association and the Mountain Plains Library Association (MPLA) in October 1986: (1) "Development of a Strategic Plan for the Harold B. Lee Library--A Model for Public Service" (Larry Ostler); (2) "The Library Committee in MPLA Libraries" (Ronald…

  2. The Leishmania infantum acidic ribosomal protein P0 administered as a DNA vaccine confers protective immunity to Leishmania major infection in BALB/c mice.

    PubMed

    Iborra, Salvador; Soto, Manuel; Carrión, Javier; Nieto, Ana; Fernández, Edgar; Alonso, Carlos; Requena, Jose M

    2003-11-01

    In this study, we examined the immunogenic properties of the Leishmania infantum acidic ribosomal protein P0 (LiP0) in the BALB/c mouse model. The humoral and cellular responses induced by the administration of the LiP0 antigen, either as soluble recombinant LiP0 (rLiP0) or as a plasmid DNA formulation (pcDNA3-LiP0), were determined. Also, the immunological response associated with a prime-boost strategy, consisting of immunization with pcDNA3-LiP0 followed by a boost with rLiP0, was assayed. Immunization with rLiP0 induced a predominant Th2-like humoral response, but no anti-LiP0 antibodies were induced after immunization with pcDNA3-LiP0, whereas a strong humoral response consisting of a mixed immunoglobulin G2a (IgG2a)-IgG1 isotype profile was induced in mice immunized with the prime-boost regime. For all three immunization protocols, rLiP0-stimulated production of gamma interferon (IFN-gamma) in both splenocytes and lymph node cells from immunized mice was observed. However, it was only when mice were immunized with pcDNA3-LiP0 that noticeable protection against L. major infection was achieved, as determined by both lesion development and parasite burden. Immunization of mice with LiP0-DNA primes both CD4(+) and CD8(+) T cells, which, with the L. major challenge, were boosted to produce significant levels of IL-12-dependent, antigen-specific IFN-gamma. Taken together, these data indicate that genetic vaccination with LiP0 induces protective immunological effector mechanisms, yet the immunological response elicited by LiP0 is not sufficient to keep the infection from progressing.

  3. Immune protection conferred by recombinant MRLC (myosin regulatory light chain) antigen in TiterMax Gold® adjuvant against experimental fasciolosis in rats.

    PubMed

    Henker, Luan C; Schwertz, Claiton I; Lucca, Neuber J; Piva, Manoela M; Prior, Keila C; Baska, Piotr; Norbury, Luke; Januszkiewicz, Kamil; Dezen, Diogenes; Duarte, Marta M M F; Moresco, Rafael N; Bertagnolli da Rosa, Liana; Mendes, Ricardo E

    2017-01-23

    Protection against experimental fasciolosis in rats immunized with recombinant myosin regulatory light chain (MRLC) in TiterMax Gold® adjuvant was assessed. The experimental trial consisted of four groups of 15 animals; group 1 was unimmunized and infected, group 2 was immunized with MRLC in adjuvant and infected, group 3 was infected and immunized with adjuvant only and group 4 was unimmunized and uninfected. Immunization with MRLC in TiterMax Gold® adjuvant (group 2) induced a reduction in fluke burdens of 51.0% (p<0.001) when compared with the adjuvant control group, and 61.5% (p<0.001) when compared with the unimmunized infected controls. There was a reduction in fecal egg output in group 2 of 44.8% and 37.3% compared with group 1 and group 3, respectively; although this difference was not statistically significant. Measurement of cytokine levels revealed higher levels of TNF-alpha and IL-2 as well as lower levels of IL-4 in group 2 during the chronic stage of infection (p<0.05), along with higher levels of IFN-gamma during early stages of infection (p<0.05). These results suggest a mixed Th1/Th2 phenotype immune response; however predominance of Th1 cytokines was observed. Levels of anti-MRLC serum IgG in group 2 were significantly higher than controls at the time of euthanasia (p<0.05). This is the first report of immunization with recombinant MRLC in rats, demonstrating that this antigen significantly reduces fluke burdens, increases the Th1 immune response and encourages further studies to improve the vaccine's efficacy.

  4. Primary Babesia rodhaini infection followed by recovery confers protective immunity against B. rodhaini reinfection and Babesia microti challenge infection in mice.

    PubMed

    Wang, Guanbo; Efstratiou, Artemis; Adjou Moumouni, Paul Franck; Liu, Mingming; Jirapattharasate, Charoonluk; Guo, Huanping; Gao, Yang; Cao, Shinuo; Zhou, Mo; Suzuki, Hiroshi; Igarashi, Ikuo; Xuan, Xuenan

    2016-10-01

    In the present study, we investigated the protective immunity against challenge infections with Babesia rodhaini and Babesia microti in the mice recovered from B. rodhaini infection. Six groups with 5 test mice in each group were used in this study, and were intraperitoneally immunized with alive and dead B. rodhaini. The challenge infections with B. rodhaini or B. microti were performed using different time courses. Our results showed that the mice recovered from primary B. rodhaini infection exhibited low parasitemia and no mortalities after the challenge infections, whereas mock mice which had received no primary infection showed a rapid increase of parasitemia and died within 7 days after the challenge with B. rodhaini. Mice immunized with dead B. rodhaini were not protected against either B. rodhaini or B. microti challenge infections, although high titers of antibody response were induced. These results indicate that only mice immunized with alive B. rodhaini could acquire protective immunity against B. rodhaini or B. microti challenge infection. Moreover, the test mice produced high levels of antibody response and low levels of cytokines (INF-γ, IL-4, IL-12, IL-10) against B. rodhaini or B. microti after challenge infection. Mock mice, however, showed rapid increases of these cytokines, which means disordered cytokines secretion occurred during the acute stage of challenge infection. The above results proved that mice immunized with alive B. rodhaini could acquire protective immunity against B. rodhaini and B. microti infections.

  5. Library 2000.

    ERIC Educational Resources Information Center

    Drake, Miriam A.

    In fall 1984, the Georgia Institute of Technology administration and library staff began planning for Library 2000, a project aimed at creating a showcase library to demonstrate the application of the latest information technology in an academic and research environment. The purposes of Library 2000 include: increasing awareness of students,…

  6. Library Buildings.

    ERIC Educational Resources Information Center

    Manley, Will; And Others

    1989-01-01

    The innovative designs of three libraries are described: the Tempe (Arizona) Public Library, which emphasizes services for children and students; an underground library at Park College, Missouri; and a public library located in the Vancouver (Washington) Mall. The fourth article describes the work going on to restore the Los Angeles (California)…

  7. Library Computing.

    ERIC Educational Resources Information Center

    Dayall, Susan A.; And Others

    1987-01-01

    Six articles on computers in libraries discuss training librarians and staff to use new software; appropriate technology; system upgrades of the Research Libraries Group's information system; pre-IBM PC microcomputers; multiuser systems for small to medium-sized libraries; and a library user's view of the traditional card catalog. (EM)

  8. Special Libraries.

    ERIC Educational Resources Information Center

    Foskett, D. J.

    The Special Library is distinguished from other libraries as being a library serving a particular group of readers, who have an existence as a group outside of their readership of the library, and whose members direct at least some of their activities towards a common purpose. Thus, the special librarian's first and major responsibility is to know…

  9. A Novel Means of Self-Protection, Unrelated to Toxin Activation, Confers Immunity to the Bactericidal Effects of the Enterococcus faecalis Cytolysin

    PubMed Central

    Coburn, Phillip S.; Hancock, Lynn E.; Booth, Mary C.; Gilmore, Michael S.

    1999-01-01

    Enterococcus faecalis has become a pervasive clinical problem due to the emergence of resistance to most antibiotics. The cytolysin of E. faecalis is a novel bacterial toxin that contributes to the severity of disease. It consists of two structural subunits, which together possess both hemolytic and bactericidal activity. Both toxin subunits are encoded in a complex operon frequently harbored on pheromone-responsive plasmids. E. faecalis strains lacking such plasmids are susceptible to the bactericidal effects of the cytolysin. A novel cytolysin immunity determinant at the 3′ end of the pAD1 cytolysin operon is described in the present study. Deletion analysis and specific mutagenesis isolated the immunity function to a single open reading frame. Specific mutagenesis experiments demonstrate that cytolysin immunity is unrelated to cytolysin activator (CylA) expression as previously proposed. Cytolysin immunity is, however, encoded on the same transcript as and 3′ to CylA, and previous associations between immunity and CylA can be ascribed to the polar behavior of Tn917 insertion. PMID:10377111

  10. Library Schools and Other Training Aspects Section. Education and Research Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library education presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Learning and Library Networks at Mid-Career Level: The Possible Contribution of the Library Schools" by M. H. Heine (United Kingdom); (2) "Network Implications for Library Education: Planning for (the)…

  11. Library Schools and Other Training Aspects Section. Education and Research Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library education presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Learning and Library Networks at Mid-Career Level: The Possible Contribution of the Library Schools" by M. H. Heine (United Kingdom); (2) "Network Implications for Library Education: Planning for (the)…

  12. 37 CFR 351.7 - Settlement conference.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 351.7 Patents, Trademarks, and Copyrights COPYRIGHT ROYALTY BOARD, LIBRARY OF CONGRESS COPYRIGHT ROYALTY JUDGES RULES AND PROCEDURES PROCEEDINGS § 351.7 Settlement conference. A post-discovery settlement conference will be held among the participants, within 21 days after the close of discovery, outside of...

  13. A Capital Venture: ALA Midwinter Conference Report.

    ERIC Educational Resources Information Center

    Wilson Library Bulletin, 1989

    1989-01-01

    This report on the American Library Association's 1989 midwinter conference summarizes the interactions and recommendations of various speakers, committees, and special interest groups. A profile of presidential candidates, summary of the most significant board and council actions, and agenda for the next conference are provided. (CLB)

  14. Immunization with Tc52 or its amino terminal domain adjuvanted with c-di-AMP induces Th17+Th1 specific immune responses and confers protection against Trypanosoma cruzi

    PubMed Central

    Matos, Marina N.; Cazorla, Silvia I.; Schulze, Kai; Ebensen, Thomas; Guzmán, Carlos A.; Malchiodi, Emilio L.

    2017-01-01

    The development of new adjuvants enables fine modulation of the elicited immune responses. Ideally, the use of one or more adjuvants should result in the induction of a protective immune response against the specific pathogen. We have evaluated the immune response and protection against Trypanosoma cruzi infection in mice vaccinated with recombinant Tc52 or its N- and C-terminal domains (NTc52 and CTc52) adjuvanted either with the STING (Stimulator of Interferon Genes) agonist cyclic di-AMP (c-di-AMP), a pegylated derivative of α-galactosylceramide (αGC-PEG), or oligodeoxynucleotides containing unmethylated CpG motifs (ODN-CpG). All groups immunized with the recombinant proteins plus adjuvant: Tc52+c-di-AMP, NTc52+c-di-AMP, CTc52+c-di-AMP, NTc52+c-di-AMP+αGC-PEG, NTc52+CpG, developed significantly higher anti-Tc52 IgG titers than controls. Groups immunized with c-di-AMP and Tc52, NTc52 or CTc52 showed the highest Tc52-specific IgA titers in nasal lavages. All groups immunized with the recombinant proteins plus adjuvant developed a strong specific cellular immune response in splenocytes and lymph node cells with significant differences for groups immunized with c-di-AMP and Tc52, NTc52 or CTc52. These groups also showed high levels of Tc52-specific IL-17 and IFN-γ producing cells, while NTc52+CpG group only showed significant difference with control in IFN-γ producing cells. Groups immunized with c-di-AMP and Tc52, NTc52 or CTc52 developed predominantly a Th17 and Th1immune response, whereas for NTc52+CpG it was a dominant Th1 response. It was previously described that αGC-PEG inhibits Th17 differentiation by activating NKT cells. Thus, in this work we have also included a group immunized with both adjuvants (NTc52+c-di-AMP+αGC-PEG) with the aim to modulate the Th17 response induced by c-di-AMP. This group showed a significant reduction in the number of Tc52-specific IL-17 producing splenocytes, as compared to the group NTc52+c-di-AMP, which has in turn

  15. Immunization with Tc52 or its amino terminal domain adjuvanted with c-di-AMP induces Th17+Th1 specific immune responses and confers protection against Trypanosoma cruzi.

    PubMed

    Matos, Marina N; Cazorla, Silvia I; Schulze, Kai; Ebensen, Thomas; Guzmán, Carlos A; Malchiodi, Emilio L

    2017-02-01

    The development of new adjuvants enables fine modulation of the elicited immune responses. Ideally, the use of one or more adjuvants should result in the induction of a protective immune response against the specific pathogen. We have evaluated the immune response and protection against Trypanosoma cruzi infection in mice vaccinated with recombinant Tc52 or its N- and C-terminal domains (NTc52 and CTc52) adjuvanted either with the STING (Stimulator of Interferon Genes) agonist cyclic di-AMP (c-di-AMP), a pegylated derivative of α-galactosylceramide (αGC-PEG), or oligodeoxynucleotides containing unmethylated CpG motifs (ODN-CpG). All groups immunized with the recombinant proteins plus adjuvant: Tc52+c-di-AMP, NTc52+c-di-AMP, CTc52+c-di-AMP, NTc52+c-di-AMP+αGC-PEG, NTc52+CpG, developed significantly higher anti-Tc52 IgG titers than controls. Groups immunized with c-di-AMP and Tc52, NTc52 or CTc52 showed the highest Tc52-specific IgA titers in nasal lavages. All groups immunized with the recombinant proteins plus adjuvant developed a strong specific cellular immune response in splenocytes and lymph node cells with significant differences for groups immunized with c-di-AMP and Tc52, NTc52 or CTc52. These groups also showed high levels of Tc52-specific IL-17 and IFN-γ producing cells, while NTc52+CpG group only showed significant difference with control in IFN-γ producing cells. Groups immunized with c-di-AMP and Tc52, NTc52 or CTc52 developed predominantly a Th17 and Th1immune response, whereas for NTc52+CpG it was a dominant Th1 response. It was previously described that αGC-PEG inhibits Th17 differentiation by activating NKT cells. Thus, in this work we have also included a group immunized with both adjuvants (NTc52+c-di-AMP+αGC-PEG) with the aim to modulate the Th17 response induced by c-di-AMP. This group showed a significant reduction in the number of Tc52-specific IL-17 producing splenocytes, as compared to the group NTc52+c-di-AMP, which has in turn

  16. Targeting the non-structural protein 1 from dengue virus to a dendritic cell population confers protective immunity to lethal virus challenge.

    PubMed

    Henriques, Hugo R; Rampazo, Eline V; Gonçalves, Antonio J S; Vicentin, Elaine C M; Amorim, Jaime H; Panatieri, Raquel H; Amorim, Kelly N S; Yamamoto, Marcio M; Ferreira, Luís C S; Alves, Ada M B; Boscardin, Silvia B

    2013-01-01

    Dengue is the most prevalent arboviral infection, affecting millions of people every year. Attempts to control such infection are being made, and the development of a vaccine is a World Health Organization priority. Among the proteins being tested as vaccine candidates in preclinical settings is the non-structural protein 1 (NS1). In the present study, we tested the immune responses generated by targeting the NS1 protein to two different dendritic cell populations. Dendritic cells (DCs) are important antigen presenting cells, and targeting proteins to maturing DCs has proved to be an efficient means of immunization. Antigen targeting is accomplished by the use of a monoclonal antibody (mAb) directed against a DC cell surface receptor fused to the protein of interest. We used two mAbs (αDEC205 and αDCIR2) to target two distinct DC populations, expressing either DEC205 or DCIR2 endocytic receptors, respectively, in mice. The fusion mAbs were successfully produced, bound to their respective receptors, and were used to immunize BALB/c mice in the presence of polyriboinosinic: polyribocytidylic acid (poly (I:C)), as a DC maturation stimulus. We observed induction of strong anti-NS1 antibody responses and similar antigen binding affinity irrespectively of the DC population targeted. Nevertheless, the IgG1/IgG2a ratios were different between mouse groups immunized with αDEC-NS1 and αDCIR2-NS1 mAbs. When we tested the induction of cellular immune responses, the number of IFN-γ producing cells was higher in αDEC-NS1 immunized animals. In addition, mice immunized with the αDEC-NS1 mAb were significantly protected from a lethal intracranial challenge with the DENV2 NGC strain when compared to mice immunized with αDCIR2-NS1 mAb. Protection was partially mediated by CD4(+) and CD8(+) T cells as depletion of these populations reduced both survival and morbidity signs. We conclude that targeting the NS1 protein to the DEC205(+) DC population with poly (I:C) opens

  17. Conference Planning.

    ERIC Educational Resources Information Center

    Burke, W. Warner, Ed.; Beckhard, Richard, Ed.

    This book, written to instruct in the use of a conference as a medium of social intercourse, is divided into four sections. Section I, which contains five articles, deals with factors to be considered in planning a conference. Specific techniques one can employ to improve a conference and several different techniques for evaluating the…

  18. Diuretics prime plant immunity in Arabidopsis thaliana.

    PubMed

    Noutoshi, Yoshiteru; Ikeda, Mika; Shirasu, Ken

    2012-01-01

    Plant activators are agrochemicals that activate the plant immune system, thereby enhancing disease resistance. Due to their prophylactic and durable effects on a wide spectrum of diseases, plant activators can provide synergistic crop protection when used in combination with traditional pest controls. Although plant activators have achieved great success in wet-rice farming practices in Asia, their use is still limited. To isolate novel plant activators applicable to other crops, we screened a chemical library using a method that can selectively identify immune-priming compounds. Here, we report the isolation and characterization of three diuretics, bumetanide, bendroflumethiazide and clopamide, as immune-priming compounds. These drugs upregulate the immunity-related cell death of Arabidopsis suspension-cultured cells induced with an avirulent strain of Pseudomonas syringae pv. tomato in a concentration-dependent manner. The application of these compounds to Arabidopsis plants confers disease resistance to not only the avirulent but also a virulent strain of the pathogen. Unlike salicylic acid, an endogenous phytohormone that governs disease resistance in response to biotrophic pathogens, the three diuretic compounds analyzed here do not induce PR1 or inhibit plant growth, showing potential as lead compounds in a practical application.

  19. Diuretics Prime Plant Immunity in Arabidopsis thaliana

    PubMed Central

    Noutoshi, Yoshiteru; Ikeda, Mika; Shirasu, Ken

    2012-01-01

    Plant activators are agrochemicals that activate the plant immune system, thereby enhancing disease resistance. Due to their prophylactic and durable effects on a wide spectrum of diseases, plant activators can provide synergistic crop protection when used in combination with traditional pest controls. Although plant activators have achieved great success in wet-rice farming practices in Asia, their use is still limited. To isolate novel plant activators applicable to other crops, we screened a chemical library using a method that can selectively identify immune-priming compounds. Here, we report the isolation and characterization of three diuretics, bumetanide, bendroflumethiazide and clopamide, as immune-priming compounds. These drugs upregulate the immunity-related cell death of Arabidopsis suspension-cultured cells induced with an avirulent strain of Pseudomonas syringae pv. tomato in a concentration-dependent manner. The application of these compounds to Arabidopsis plants confers disease resistance to not only the avirulent but also a virulent strain of the pathogen. Unlike salicylic acid, an endogenous phytohormone that governs disease resistance in response to biotrophic pathogens, the three diuretic compounds analyzed here do not induce PR1 or inhibit plant growth, showing potential as lead compounds in a practical application. PMID:23144763

  20. Finding Common Ground: Creating the Library of the Future without Diminishing the Library of the Past. The New Library Series.

    ERIC Educational Resources Information Center

    LaGuardia, Cheryl, Ed.; Mitchell, Barbara A., Ed.

    Adapting today's libraries to better serve tomorrow's needs challenges even the most forward-thinking library staff. This book offers practical, workable solutions from several authorities in the industry. Fifty-five conference papers are included and divided into the following subject areas: "Technology and the Network: Daimons or Demons?";…

  1. Bee venom phospholipase A2 induces a primary type 2 response that is dependent on the receptor ST2 and confers protective immunity

    PubMed Central

    Palm, Noah W.; Rosenstein, Rachel K.; Yu, Shuang; Schenten, Dominik; Florsheim, Esther; Medzhitov, Ruslan

    2013-01-01

    SUMMARY Venoms consist of toxic components that are delivered to their victims via bites or stings. Venoms also represent a major class of allergens in humans. Phospholipase A2 (PLA2) is a conserved component of venoms from multiple species and is the major allergen in bee venom. Here we examined how bee venom PLA2 is sensed by the innate immune system and induces a type 2 immune response in mice. We found that bee venom PLA2 induced a T helper type 2 (Th2) cell-type response and group 2 innate lymphoid cell activation via the enzymatic cleavage of membrane phospholipids and release of interleukin-33. Furthermore, we showed that the IgE response to PLA2 could protect mice from future challenge with a near-lethal dose of PLA2. These data suggest that the innate immune system can detect the activity of a conserved component of venoms and induce a protective immune response against a venom toxin. PMID:24210353

  2. Acellular pertussis vaccine based on outer membrane vesicles capable of conferring both long-lasting immunity and protection against different strain genotypes.

    PubMed

    Gaillard, María Emilia; Bottero, Daniela; Errea, Agustina; Ormazábal, Maximiliano; Zurita, M Eugenia; Moreno, Griselda; Rumbo, Martin; Castuma, Celina; Bartel, Erika; Flores, Dario; van der Ley, Peter; van der Ark, Arno; F Hozbor, Daniela

    2014-02-12

    Despite high vaccination coverage rates, pertussis continues to be a global concern, with increased incidence widely noted. The current pertussis epidemiologic situation has been mainly attributed to waning immunity and pathogen adaptation. To improve the disease control, a new generation of vaccines capable to overcome those weaknesses associated to the current vaccines need to be developed. Previously we have demonstrated that the outer membrane vesicles obtained from the recombinant Bordetella pertussis strain expressing PagL enzyme (OMVs(BpPagL)) are good vaccine candidates to protect against pertussis. In this work the OMVs(BpPagL) formulated with diphtheria and tetanus toxoids (Tdap(OMVsBpPagL)) was used to evaluate its capacity to offer protection against Argentinean clinical isolates and to induce long-term immunity. To these aims BALB/c mice were immunized with Tdap(OMVsBpPagL) and challenged with sublethal doses of the clinical isolate Bp106 selected as a representative circulating isolate. Comparisons with a current commercial Tdap vaccine used at a dose in which pertussis toxin level was equivalent to that of Tdap(OMVsBpPagL) were performed. With the normalized doses of both vaccines we observed that Tdap(OMVsBpPagL) protected against the clinical isolate infection, whereas current commercial Tdap vaccine showed little protection against such pathogen. Regarding long-term immunity we observed that the Tdap(OMVsBpPagL) protective capacity against the recommended WHO reference strain persisted at least 9 months. In agreement with these results Tdap(OMVsBpPagL) induced Th1 and Th2 immune response. In contrast, commercial Tdap induced Th2 but weak Th1 responses. All results presented here showed that Tdap(OMVsBpPagL) is an interesting formulation to be considered for the development of novel acellular multi-antigen vaccine. Copyright © 2013 Elsevier Ltd. All rights reserved.

  3. 99th Dahlem Conference on Infection, Inflammation and Chronic Inflammatory Disorders: Immune therapies of type 1 diabetes: new opportunities based on the hygiene hypothesis

    PubMed Central

    Chatenoud, L; You, S; Okada, H; Kuhn, C; Michaud, B; Bach, J-F

    2010-01-01

    Insulin-dependent (type 1) diabetes is a prototypic organ-specific autoimmune disease resulting from the selective destruction of insulin-secreting β cells within pancreatic islets of Langerhans by an immune-mediated inflammation involving autoreactive CD4+ and CD8+ T lymphocytes which infiltrate pancreatic islets. Current treatment is substitutive, i.e. chronic use of exogenous insulin which, in spite of significant advances, is still associated with major constraints (multiple daily injections, risks of hypoglycaemia) and lack of effectiveness over the long term in preventing severe degenerative complications. Finding a cure for autoimmune diabetes by establishing effective immune-based therapies is a real medical health challenge, as the disease incidence increases steadily in industrialized countries. As the disease affects mainly children and young adults, any candidate immune therapy must therefore be safe and avoid a sustained depression of immune responses with the attendant problems of recurrent infection and drug toxicity. Thus, inducing or restoring immune tolerance to target autoantigens, controlling the pathogenic response while preserving the host reactivity to exogenous/unrelated antigens, appears to be the ideal approach. Our objective is to review the major progress accomplished over the last 20 years towards that aim. In addition, we would like to present another interesting possibility to access new preventive strategies based on the ‘hygiene hypothesis’, which proposes a causal link between the increasing incidence of autoimmune diseases, including diabetes, and the decrease of the infectious burden. The underlying rationale is to identify microbial-derived compounds mediating the protective activity of infections which could be developed therapeutically. PMID:20415859

  4. SLA's Conference Wrap-Up.

    ERIC Educational Resources Information Center

    Shepherd, Susan J., Comp.

    1987-01-01

    Encapsulates a wrap-up session on technical programs presented at the Special Libraries Association's June 1987 conference, including presentations in the areas of information technology, management, education, and international issues. Ethics, value, leadership, international issues, image, and risk are identified as major themes of the…

  5. Social Science Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on social science documentation and information services presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Problems in the Availability of Some Social Science Publications," a discussion by Maurice B. Line (United Kingdom) of bibliographic control of fugitive literature in the…

  6. Children's Libraries Section. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on children's services, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "Education and Training of Children's Librarians: An Investigation" by Eva Glistrup (Denmark), which presents preliminary results from a 1982 survey of institutions in 30 countries (30% response…

  7. Children's Libraries Section. Libraries Serving the General Public Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on children's services, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "Education and Training of Children's Librarians: An Investigation" by Eva Glistrup (Denmark), which presents preliminary results from a 1982 survey of institutions in 30 countries (30% response…

  8. VA Library Service--Today's look at Tomorrow's Library.

    ERIC Educational Resources Information Center

    Veterans Administration, Washington, DC.

    The Conference Poceedings are divided into three broad topics: systems planning, audiovisuals in biomedical communication, and automation and networking. Speakers from within the Veterans Administration (VA), from the National Medical Audiovisual Center, and the Lister Hill National Center for Biomedical Communications, National Library of…

  9. Geography and Map Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on geography and map librarianship presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Geographical Canada-Research and the Network of Canada Collections in the Federal Republic of Germany" by Hermann Gunzel, which describes the history of German research in Canadian geography,…

  10. Geography and Map Libraries Section. Special Libraries Division. Papers.

    ERIC Educational Resources Information Center

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on geography and map librarianship presented at the 1982 International Federation of Library Associations (IFLA) conference include: (1) "Geographical Canada-Research and the Network of Canada Collections in the Federal Republic of Germany" by Hermann Gunzel, which describes the history of German research in Canadian geography,…

  11. Professional development through attending conferences: reflections of a health librarian.

    PubMed

    Jenkins, Ruth

    2015-06-01

    In this article, guest writer Ruth Jenkins from Berkshire Heathcare Foundation Trust reflects on two conferences she attended in 2014, LILAC and SLA. Through the process of reflection, she considers the benefits that attending conferences can have to library and information professionals in the health sector. In particular, she discusses the opportunities and areas for learning and professional development that conferences can offer including evidence-based practice and current awareness, gaining new knowledge and objectivity, and networking and the unexpected benefits of conferences. Ruth also offers some practical hints and tips on ways to facilitate your attendance at conferences, including through awards and funding. H.S. © 2015 Health Libraries Group.

  12. Brucella abortusΔcydCΔcydD and ΔcydCΔpurD double-mutants are highly attenuated and confer long-term protective immunity against virulent Brucella abortus.

    PubMed

    Truong, Quang Lam; Cho, Youngjae; Park, Soyeon; Kim, Kiju; Hahn, Tae-Wook

    2016-01-04

    We constructed double deletion (ΔcydCΔcydD and ΔcydCΔpurD) mutants from virulent Brucella abortus biovar 1 field isolate (BA15) by deleting the genes encoding an ATP-binding cassette-type transporter (cydC and cydD genes) and a phosphoribosylamine-glycine ligase (purD). Both BA15ΔcydCΔcydD and BA15ΔcydCΔpurD double-mutants exhibited significant attenuation of virulence when assayed in murine macrophages or in BALB/c mice. Both double-mutants were readily cleared from spleens by 4 weeks post-inoculation even when inoculated at the dose of 10(8) CFU per mouse. Moreover, the inoculated mice showed no splenomegaly, which indicates that the mutants are highly attenuated. Importantly, the attenuation of in vitro and in vivo growth did not impair the ability of these mutants to confer long-term protective immunity in mice against challenge with B. abortus strain 2308. Vaccination of mice with either mutant induced humoral and cell-mediated immune responses, and provided significantly better protection than commercial B. abortus strain RB51 vaccine. These results suggest that highly attenuated BA15ΔcydCΔcydD and BA15ΔcydCΔpurD mutants can be used effectively as potential live vaccine candidates against bovine brucellosis.

  13. The protoxin Cry1Ac of Bacillus thuringiensis improves the protection conferred by intranasal immunization with Brucella abortus RB51 in a mouse model.

    PubMed

    González-González, Edith; García-Hernández, Ana Lilia; Flores-Mejía, Raúl; López-Santiago, Rubén; Moreno-Fierros, Leticia

    2015-02-25

    Brucellosis is a zoonotic disease affecting many people and animals worldwide. Preventing this infection requires improving vaccination strategies. The protoxin Cry1Ac of Bacillus thuringiensis is an adjuvant that, in addition to increasing the immunogenicity of different antigens, has shown to be protective in different models of parasitic infections. The objective of the present study was to test whether the intranasal co-administration of pCry1Ac with the RB51 vaccine strain of Brucella abortus confers protection against an intranasal challenge with the virulent strain B. abortus 2308 in BALB/c mice. The results showed that co-administration of pCry1Ac and RB51, increased the immunoprotection conferred by the vaccine as evidenced by the following: (1) decrease of the splenic bacterial load when challenged intranasally with the virulent strain; (2) greater in vivo cytotoxic activity in response to the transference of previously infected cells; (3) further proliferation of cytotoxic TCD8+ cells in response to stimulation with heat-inactivated bacteria; (4) increased production of TNF-α and IFN-γ; and (5) significant IgG2a response. These results indicate that the use of the Cry1Ac protein as a mucosal adjuvant via the intranasal route can be a promising alternative for improving current RB51 vaccine against brucellosis.

  14. Immunization with recombinant bivalent chimera r-Cpae confers protection against alpha toxin and enterotoxin of Clostridium perfringens type A in murine model.

    PubMed

    Shreya, Das; Uppalapati, Siva R; Kingston, Joseph J; Sripathy, Murali H; Batra, Harsh V

    2015-05-01

    Clostridium perfringens type A, an anaerobic pathogen is the most potent cause of soft tissue infections like gas gangrene and enteric diseases like food poisoning and enteritis. The disease manifestations are mediated via two important exotoxins, viz. myonecrotic alpha toxin (αC) and enterotoxin (CPE). In the present study, we synthesized a bivalent chimeric protein r-Cpae comprising C-terminal binding regions of αC and CPE using structural vaccinology rationale and assessed its protective efficacy against both alpha toxin (αC) and enterotoxin (CPE) respectively, in murine model. Active immunization of mice with r-Cpae generated high circulating serum IgG (systemic), significantly increased intestinal mucosal s-IgA antibody titres and resulted in substantial protection to the immunized animals (100% and 75% survival) with reduced tissue morbidity when administered with 5×LD(100) doses of αC (intramuscular) and CPE (intra-gastric gavage) respectively. Mouse RBCs and Caco-2 cells incubated with a mixture of anti-r-Cpae antibodies and αC and CPE respectively, illustrated significantly higher protection against the respective toxins. Passive immunization of mice with a similar mixture resulted in 91-100% survival at the end of the 15 days observation period while mice immunized with a concoction of sham sera and respective toxins died within 2-3 days. This work demonstrates the efficacy of the rationally designed r-Cpae chimeric protein as a potential sub unit vaccine candidate against αC and CPE of C. perfringens type A toxemia. Copyright © 2015 Elsevier Ltd. All rights reserved.

  15. Immunization with a novel Clostridium perfringens epsilon toxin mutant rETXY196E-C confers strong protection in mice

    PubMed Central

    Yao, Wenwu; Kang, Jingjing; Kang, Lin; Gao, Shan; Yang, Hao; Ji, Bin; Li, Ping; Liu, Jing; Xin, Wenwen; Wang, Jinglin

    2016-01-01

    Epsilon toxin (ETX) is produced by toxinotypes B and D of Clostridium perfringens. It can induce lethal enterotoxemia in domestic animals, mainly in sheep, goats and cattle, causing serious economic losses to global animal husbandry. In this study, a novel and stable epsilon toxin mutant rETXY196E-C, obtained by substituting the 196th tyrosine (Y196) with glutamic acid (E) and introducing of 23 amino acids long C-terminal peptide, was determined as a promising recombinant vaccine candidate against enterotoxemia. After the third vaccination, the antibody titers against recombinant wild type (rETX) could reach 1:105 in each immunized group, and the mice were completely protected from 100 × LD50 (50% lethal dose) of rETX challenge. The mice in 15 μg subcutaneously immunized group fully survived at the dose of 500 × LD50 of rETX challenge and 80% of mice survived at 180 μg (1000 × LD50) of rETX administration. In vitro, immune sera from 15 μg subcutaneously immunized group could completely protect MDCK cells from 16 × CT50 (50% lethal dose of cells) of rETX challenge and protect against 10 × LD50 dose (1.8 μg) of rETX challenge in mice. These data suggest that recombinant protein rETXY196E-C is a potential vaccine candidate for future applied researches. PMID:27048879

  16. Immunization with a novel Clostridium perfringens epsilon toxin mutant rETX(Y196E)-C confers strong protection in mice.

    PubMed

    Yao, Wenwu; Kang, Jingjing; Kang, Lin; Gao, Shan; Yang, Hao; Ji, Bin; Li, Ping; Liu, Jing; Xin, Wenwen; Wang, Jinglin

    2016-04-06

    Epsilon toxin (ETX) is produced by toxinotypes B and D of Clostridium perfringens. It can induce lethal enterotoxemia in domestic animals, mainly in sheep, goats and cattle, causing serious economic losses to global animal husbandry. In this study, a novel and stable epsilon toxin mutant rETX(Y196E)-C, obtained by substituting the 196th tyrosine (Y196) with glutamic acid (E) and introducing of 23 amino acids long C-terminal peptide, was determined as a promising recombinant vaccine candidate against enterotoxemia. After the third vaccination, the antibody titers against recombinant wild type (rETX) could reach 1:10(5) in each immunized group, and the mice were completely protected from 100 × LD50 (50% lethal dose) of rETX challenge. The mice in 15 μg subcutaneously immunized group fully survived at the dose of 500 × LD50 of rETX challenge and 80% of mice survived at 180 μg (1000 × LD50) of rETX administration. In vitro, immune sera from 15 μg subcutaneously immunized group could completely protect MDCK cells from 16 × CT50 (50% lethal dose of cells) of rETX challenge and protect against 10 × LD50 dose (1.8 μg) of rETX challenge in mice. These data suggest that recombinant protein rETX(Y196E)-C is a potential vaccine candidate for future applied researches.

  17. Immunization with a chimera consisting of the B subunit of Shiga toxin type 2 and brucella lumazine synthase confers total protection against Shiga toxins in mice.

    PubMed

    Mejias, María P; Ghersi, Giselle; Craig, Patricio O; Panek, Cecilia A; Bentancor, Leticia V; Baschkier, Ariela; Goldbaum, Fernando A; Zylberman, Vanesa; Palermo, Marina S

    2013-09-01

    The striking feature of enterohemorrhagic Escherichia coli (EHEC) infections is the production of Shiga toxins (Stx) implicated in the development of the life-threatening hemolytic uremic syndrome. Despite the magnitude of the social impact of EHEC infections, no licensed vaccine or effective therapy is available for human use. One of the biggest challenges is to develop an effective and safe immunogen to ensure nontoxicity, as well as a strong input to the immune system to induce long-lasting, high-affinity Abs with anti-Stx-neutralizing capacity. The enzyme lumazine synthase from Brucella spp. (BLS) is a highly stable dimer of pentamers and a scaffold with enormous plasticity on which to display foreign Ags. Taking into account the advantages of BLS and the potential capacity of the B subunit of Stx2 to induce Abs that prevent Stx2 toxicity by blocking its entrance into the host cells, we engineered a new immunogen by inserting the B subunit of Stx2 at the amino termini of BLS. The resulting chimera demonstrated a strong capacity to induce a long-lasting humoral immune response in mice. The chimera induced Abs with high neutralizing capacity for Stx2 and its variants. Moreover, immunized mice were completely protected against i.v. Stx2 challenge, and weaned mice receiving an oral challenge with EHEC were completely protected by the transference of immune sera. We conclude that this novel immunogen represents a promising candidate for vaccine or Ab development with preventive or therapeutic ends, for use in hemolytic uremic syndrome-endemic areas or during future outbreaks caused by pathogenic strains of Stx-producing E. coli.

  18. DNA vaccine encoding type IV pilin of Actinobacillus pleuropneumoniae induces strong immune response but confers limited protective efficacy against serotype 2 challenge.

    PubMed

    Lu, Yu-Chun; Li, Min-Chen; Chen, Yi-Min; Chu, Chun-Yen; Lin, Shuen-Fuh; Yang, Wen-Jen

    2011-10-13

    Actinobacillus pleuropneumoniae is a gram-negative bacterial pathogen that causes swine pleuropneumonia, a highly contagious and often fatal disease that occurs worldwide. Our previous study showed that DNA vaccines encoding Apx exotoxin structural proteins ApxIA and/or ApxIIA, are a promising novel approach for immunization against the lethal challenge of A. pleuropneumoniae serotype 1. Vaccination against A. pleuropneumoniae is impeded by the lack of vaccines inducing reliable cross-serotype protection. Type IV fimbrial protein ApfA has been shown to be present and highly conserved in various serotypes of A. pleuropneumoniae. A novel DNA vaccine encoding ApfA (pcDNA-apfA) was constructed to evaluate the protective efficacy against infection with A. pleuropneumoniae serotype 2. A significant antibody response against pilin was generated following pcDNA-apfA immunization, suggesting that it was expressed in vivo. The IgG subclass (IgG1 and IgG2a) analysis indicates that the pcDNA-apfA vaccine induces both Th1 and Th2 immune responses. The IgA analysis shows that mucosal immunity could be enhanced by this DNA vaccine. Nevertheless, the strong antibody response induced by pcDNA-apfA vaccine only provided limited 30% protective efficacy against the serotype 2 challenge. These results in this study do not coincide with that the utility of type IV pilin is a good vaccine candidate against other infectious pathogens. It indicates that pilin should play a limited role in the development of a vaccine against A. pleuropneumoniae infection.

  19. Handling Special Materials in Libraries.

    ERIC Educational Resources Information Center

    Kaiser, Frances E., Ed.

    Libraries of all kinds need and collect special materials for use by their patrons--government publications, technical reports, maps, proprietary publications, company and trade literature, patents and trademarks, standards, symposia and conference records, and related miscellaneous publications. This collection of papers serves as a guide to the…

  20. Handling Special Materials in Libraries.

    ERIC Educational Resources Information Center

    Kaiser, Frances E., Ed.

    Libraries of all kinds need and collect special materials for use by their patrons--government publications, technical reports, maps, proprietary publications, company and trade literature, patents and trademarks, standards, symposia and conference records, and related miscellaneous publications. This collection of papers serves as a guide to the…