Sample records for liferaft data-driven batch

  1. 46 CFR 160.151-13 - Fabrication of prototype inflatable liferafts for approval.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Fabrication of prototype inflatable liferafts for... Liferafts (SOLAS) § 160.151-13 Fabrication of prototype inflatable liferafts for approval. If the... Commandant, fabrication of a prototype inflatable liferaft must proceed in the following sequence: (a) The...

  2. 46 CFR 160.151-31 - Production inspections and tests of inflatable liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... capacity. (2) A new lot must begin whenever the liferafts undergo changes of design, material, production... 46 Shipping 6 2010-10-01 2010-10-01 false Production inspections and tests of inflatable liferafts... Liferafts (SOLAS) § 160.151-31 Production inspections and tests of inflatable liferafts. (a) Production...

  3. 46 CFR 108.545 - Marine evacuation system launching arrangements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... associated liferafts. Inflatable liferafts used in conjunction with the marine evacuation system must be stowed as follows: (1) Each inflatable liferaft used in conjunction with the marine evacuation system... platform. (2) Each inflatable liferaft used in conjunction with the marine evacuation system must be...

  4. 46 CFR 131.865 - Inflatable liferafts and inflatable buoyant apparatus.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Inflatable liferafts and inflatable buoyant apparatus... SUPPLY VESSELS OPERATIONS Markings for Fire Equipment and Emergency Equipment § 131.865 Inflatable liferafts and inflatable buoyant apparatus. The number of the inflatable liferaft or inflatable buoyant...

  5. 46 CFR 131.865 - Inflatable liferafts and inflatable buoyant apparatus.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Inflatable liferafts and inflatable buoyant apparatus... SUPPLY VESSELS OPERATIONS Markings for Fire Equipment and Emergency Equipment § 131.865 Inflatable liferafts and inflatable buoyant apparatus. The number of the inflatable liferaft or inflatable buoyant...

  6. 46 CFR 133.145 - Marine evacuation system launching arrangements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... liferafts. Inflatable liferafts used in conjunction with the marine evacuation system must be stowed as follows: (1) Each inflatable liferaft used in conjunction with the marine evacuation system must be close.... (2) Each inflatable liferaft used in conjunction with the marine evacuation system must be capable of...

  7. 46 CFR 160.051-5 - Design and performance of Coastal Service inflatable liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Design and performance of Coastal Service inflatable... Liferafts for Domestic Service § 160.051-5 Design and performance of Coastal Service inflatable liferafts. To obtain Coast Guard approval, each Coastal Service inflatable liferaft must comply with subpart 160...

  8. Parameterized data-driven fuzzy model based optimal control of a semi-batch reactor.

    PubMed

    Kamesh, Reddi; Rani, K Yamuna

    2016-09-01

    A parameterized data-driven fuzzy (PDDF) model structure is proposed for semi-batch processes, and its application for optimal control is illustrated. The orthonormally parameterized input trajectories, initial states and process parameters are the inputs to the model, which predicts the output trajectories in terms of Fourier coefficients. Fuzzy rules are formulated based on the signs of a linear data-driven model, while the defuzzification step incorporates a linear regression model to shift the domain from input to output domain. The fuzzy model is employed to formulate an optimal control problem for single rate as well as multi-rate systems. Simulation study on a multivariable semi-batch reactor system reveals that the proposed PDDF modeling approach is capable of capturing the nonlinear and time-varying behavior inherent in the semi-batch system fairly accurately, and the results of operating trajectory optimization using the proposed model are found to be comparable to the results obtained using the exact first principles model, and are also found to be comparable to or better than parameterized data-driven artificial neural network model based optimization results. Copyright © 2016 ISA. Published by Elsevier Ltd. All rights reserved.

  9. 46 CFR 160.151-27 - Approval inspections and tests for inflatable liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...; and paragraph 5.17, a prototype inflatable liferaft of each design submitted for Coast Guard approval... laboratory's inspection of the prototype liferaft under § 160.151-13(a) satisfies the requirements of...

  10. Data-driven monitoring for stochastic systems and its application on batch process

    NASA Astrophysics Data System (ADS)

    Yin, Shen; Ding, Steven X.; Haghani Abandan Sari, Adel; Hao, Haiyang

    2013-07-01

    Batch processes are characterised by a prescribed processing of raw materials into final products for a finite duration and play an important role in many industrial sectors due to the low-volume and high-value products. Process dynamics and stochastic disturbances are inherent characteristics of batch processes, which cause monitoring of batch processes a challenging problem in practice. To solve this problem, a subspace-aided data-driven approach is presented in this article for batch process monitoring. The advantages of the proposed approach lie in its simple form and its abilities to deal with stochastic disturbances and process dynamics existing in the process. The kernel density estimation, which serves as a non-parametric way of estimating the probability density function, is utilised for threshold calculation. An industrial benchmark of fed-batch penicillin production is finally utilised to verify the effectiveness of the proposed approach.

  11. 46 CFR 160.151-53 - Notice to OCMI of servicing.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... liferaft to be serviced— (1) The make and size of the liferaft; (2) The age of the liferaft; and (3... arrange for the servicing to be witnessed instead by a third-party inspector accepted by the OCMI if a...

  12. Effectiveness of LifeRAFT Undergraduate Helping Skills Training Model

    ERIC Educational Resources Information Center

    Campbell, Elizabeth L.; Davidson, Kenzie; Davidson, Spencer M.

    2017-01-01

    LifeRAFT, a helping skills training model for undergraduate paraprofessionals, addresses training needs for applied psychology skills for undergraduate psychology majors. LifeRAFT draws from three empirically supported psychotherapy treatments to introduce counselling theory and encourage helping skill progression. Trainees learn practical helping…

  13. 46 CFR 160.151-25 - Additional equipment for inflatable liferafts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Additional equipment for inflatable liferafts. 160.151-25 Section 160.151-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts (SOLAS...

  14. 46 CFR 160.051-9 - Equipment required for Coastal Service inflatable liferafts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Equipment required for Coastal Service inflatable...) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts for Domestic Service § 160.051-9 Equipment required for Coastal Service inflatable liferafts. In...

  15. 46 CFR 108.530 - Stowage of survival craft.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... craft required to be served by a launching appliance or marine evacuation system must be stowed as.... (5) Each rigid container for an inflatable liferaft to be launched by a launching appliance must be... arranged for float-free launching. The arrangement must ensure that the liferaft or liferafts when released...

  16. 46 CFR 169.819 - Manning of lifeboats and liferafts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Manning of lifeboats and liferafts. 169.819 Section 169.819 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Operations § 169.819 Manning of lifeboats and liferafts. (a) The provisions of this section shall...

  17. 46 CFR 169.819 - Manning of lifeboats and liferafts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Manning of lifeboats and liferafts. 169.819 Section 169.819 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Operations § 169.819 Manning of lifeboats and liferafts. (a) The provisions of this section shall...

  18. 46 CFR 169.819 - Manning of lifeboats and liferafts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Manning of lifeboats and liferafts. 169.819 Section 169.819 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Operations § 169.819 Manning of lifeboats and liferafts. (a) The provisions of this section shall...

  19. 46 CFR 169.819 - Manning of lifeboats and liferafts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Manning of lifeboats and liferafts. 169.819 Section 169.819 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Operations § 169.819 Manning of lifeboats and liferafts. (a) The provisions of this section shall...

  20. 46 CFR 199.203 - Marshalling of liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... LIFESAVING SYSTEMS FOR CERTAIN INSPECTED VESSELS Additional Requirements for Passenger Vessels § 199.203 Marshalling of liferafts. (a) Each passenger vessel must have a lifeboat or rescue boat for each six liferafts when— (1) Each lifeboat and rescue boat is loaded with its full complement of persons; and (2) The...

  1. 46 CFR 131.580 - Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... lifejackets, inflatable buoyant apparatus, and inflated rescue boats. 131.580 Section 131.580 Shipping COAST... Inspections § 131.580 Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant apparatus must be serviced...

  2. 46 CFR 131.580 - Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... lifejackets, inflatable buoyant apparatus, and inflated rescue boats. 131.580 Section 131.580 Shipping COAST... Inspections § 131.580 Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant apparatus must be serviced...

  3. 46 CFR 169.819 - Manning of lifeboats and liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Manning of lifeboats and liferafts. 169.819 Section 169.819 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) NAUTICAL SCHOOLS SAILING SCHOOL VESSELS Operations § 169.819 Manning of lifeboats and liferafts. (a) The provisions of this section shall apply to all vessels equipped with...

  4. 46 CFR 160.051-7 - Design and performance of Coastal Service inflatable liferafts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 6 2013-10-01 2013-10-01 false Design and performance of Coastal Service inflatable liferafts. 160.051-7 Section 160.051-7 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts for Domestic Service § 160.051-7...

  5. 46 CFR 160.051-5 - Design and performance of Coastal Service inflatable liferafts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Design and performance of Coastal Service inflatable liferafts. 160.051-5 Section 160.051-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts for Domestic Service § 160.051-5...

  6. 46 CFR 160.051-7 - Design and performance of Coastal Service inflatable liferafts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 6 2014-10-01 2014-10-01 false Design and performance of Coastal Service inflatable liferafts. 160.051-7 Section 160.051-7 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts for Domestic Service § 160.051-7...

  7. 46 CFR 160.051-7 - Design and performance of A and B inflatable liferafts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Design and performance of A and B inflatable liferafts. 160.051-7 Section 160.051-7 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts for Domestic Service § 160.051-7 Design and...

  8. 46 CFR 160.051-9 - Design and performance of A and B inflatable liferafts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 6 2013-10-01 2013-10-01 false Design and performance of A and B inflatable liferafts. 160.051-9 Section 160.051-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts for Domestic Service § 160.051-9 Design and...

  9. 46 CFR 160.051-9 - Design and performance of A and B inflatable liferafts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 6 2014-10-01 2014-10-01 false Design and performance of A and B inflatable liferafts. 160.051-9 Section 160.051-9 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts for Domestic Service § 160.051-9 Design and...

  10. 46 CFR 160.151-17 - Additional requirements for design and performance of SOLAS A and SOLAS B inflatable liferafts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Additional requirements for design and performance of SOLAS A and SOLAS B inflatable liferafts. 160.151-17 Section 160.151-17 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts ...

  11. 46 CFR 160.051-7 - Design and performance of A and B inflatable liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Design and performance of A and B inflatable liferafts. 160.051-7 Section 160.051-7 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts for Domestic Service § 160.051-7 Design and...

  12. 46 CFR 169.849 - Posting placards containing instructions for launching and inflating inflatable liferafts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... accessible to the ship's company and guests approved placards containing instructions for launching and inflating inflatable liferafts. The number and location of such placards for a particular vessel shall be...

  13. 46 CFR 169.849 - Posting placards containing instructions for launching and inflating inflatable liferafts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... accessible to the ship's company and guests approved placards containing instructions for launching and inflating inflatable liferafts. The number and location of such placards for a particular vessel shall be...

  14. 46 CFR 169.849 - Posting placards containing instructions for launching and inflating inflatable liferafts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... accessible to the ship's company and guests approved placards containing instructions for launching and inflating inflatable liferafts. The number and location of such placards for a particular vessel shall be...

  15. 46 CFR 169.849 - Posting placards containing instructions for launching and inflating inflatable liferafts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... accessible to the ship's company and guests approved placards containing instructions for launching and inflating inflatable liferafts. The number and location of such placards for a particular vessel shall be...

  16. 46 CFR 160.151-43 - Conditions at servicing facilities.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... liferaft, can be easily cleaned, and is kept clean and free from oil, grease, and abrasive material; (6) Is... areas or rooms for storage of liferafts awaiting servicing, repair, or delivery; for repair and painting...

  17. 46 CFR 160.151-43 - Conditions at servicing facilities.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... liferaft, can be easily cleaned, and is kept clean and free from oil, grease, and abrasive material; (6) Is... areas or rooms for storage of liferafts awaiting servicing, repair, or delivery; for repair and painting...

  18. 46 CFR 160.151-43 - Conditions at servicing facilities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... liferaft, can be easily cleaned, and is kept clean and free from oil, grease, and abrasive material; (6) Is... areas or rooms for storage of liferafts awaiting servicing, repair, or delivery; for repair and painting...

  19. 46 CFR 160.151-43 - Conditions at servicing facilities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... liferaft, can be easily cleaned, and is kept clean and free from oil, grease, and abrasive material; (6) Is... areas or rooms for storage of liferafts awaiting servicing, repair, or delivery; for repair and painting...

  20. 46 CFR 160.151-15 - Design and performance of inflatable liferafts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... to differences in thermal expansion, freezing, buckling, galvanic corrosion, or other... lb), unless the liferaft is intended for launching into the water directly from its stowed position... container must be inherently resistant, or treated to be resistant, to deterioration from sunlight and salt...

  1. 14 CFR 25.1411 - General.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Liferafts. (1) The stowage provisions for the liferafts described in § 25.1415 must accommodate enough rafts... must be stowed near exits through which the rafts can be launched during an unplanned ditching. (3) Rafts automatically or remotely released outside the airplane must be attached to the airplane by means...

  2. 14 CFR 25.1415 - Ditching equipment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... operating rules of this chapter, must meet the requirements of this section. (b) Each liferaft and each life... liferaft. (d) There must be an approved survival type emergency locator transmitter for use in one life raft. (e) For airplanes not certificated for ditching under § 25.801 and not having approved life...

  3. 46 CFR 160.151-45 - Equipment required for servicing facilities.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...); (d) Hot presses (if applicable); (e) Safety-type glue pots or equivalents; (f) Abrasive devices; (g..., or other pressure-measurement device or pressure gauge of equivalent accuracy and sensitivity; (j... liferafts, unless the facility services only non-davit-launched liferafts; (q) A supply of parts for all...

  4. 46 CFR 131.580 - Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... lifejackets, inflatable buoyant apparatus, and inflated rescue boats. 131.580 Section 131.580 Shipping COAST..., and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant apparatus must be serviced... maintenance of inflatable rescue boats must follow the manufacturers' instructions. Each repair, except an...

  5. 46 CFR 131.580 - Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... lifejackets, inflatable buoyant apparatus, and inflated rescue boats. 131.580 Section 131.580 Shipping COAST..., and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant apparatus must be serviced... maintenance of inflatable rescue boats must follow the manufacturers' instructions. Each repair, except an...

  6. 46 CFR 131.580 - Servicing of inflatable liferafts, inflatable lifejackets, inflatable buoyant apparatus, and...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... lifejackets, inflatable buoyant apparatus, and inflated rescue boats. 131.580 Section 131.580 Shipping COAST..., and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant apparatus must be serviced... maintenance of inflatable rescue boats must follow the manufacturers' instructions. Each repair, except an...

  7. 46 CFR 160.151-15 - Design and performance of inflatable liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...-15 Section 160.151-15 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT... structural components. (b) Seams (Regulation III/30.2.1). Each seam must be at least as strong as the weakest... is greater. (k) Painter system (Regulation III/38.6.1). The painter protruding from the liferaft...

  8. 46 CFR 160.151-15 - Design and performance of inflatable liferafts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... deterioration from sunlight and salt spray, and resistant to absorption and wicking of water. (l) Inflation..., buckling, galvanic corrosion, or other incompatibilities. (e) Color (IMO LSA Code, as amended by Resolution... may not exceed 185 kg (407.8 lb), unless the liferaft is intended for launching into the water...

  9. 46 CFR 160.151-15 - Design and performance of inflatable liferafts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... deterioration from sunlight and salt spray, and resistant to absorption and wicking of water. (l) Inflation..., buckling, galvanic corrosion, or other incompatibilities. (e) Color (IMO LSA Code, as amended by Resolution... may not exceed 185 kg (407.8 lb), unless the liferaft is intended for launching into the water...

  10. 46 CFR 160.151-15 - Design and performance of inflatable liferafts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... deterioration from sunlight and salt spray, and resistant to absorption and wicking of water. (l) Inflation..., buckling, galvanic corrosion, or other incompatibilities. (e) Color (IMO LSA Code, as amended by Resolution... may not exceed 185 kg (407.8 lb), unless the liferaft is intended for launching into the water...

  11. 46 CFR 160.051-3 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... requirements prescribed in subpart 160.151 for a SOLAS A or SOLAS B liferaft, respectively, except that the... means a liferaft that does not meet the all of the requirements prescribed in subpart 160.151 for a...-ENG-4), Attn: Lifesaving and Fire Safety Division, U.S. Coast Guard Stop 7509, 2703 Martin Luther King...

  12. 46 CFR 160.051-3 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... requirements prescribed in subpart 160.151 for a SOLAS A or SOLAS B liferaft, respectively, except that the... means a liferaft that does not meet the all of the requirements prescribed in subpart 160.151 for a...-ENG-4), Attn: Lifesaving and Fire Safety Division, U.S. Coast Guard Stop 7509, 2703 Martin Luther King...

  13. 46 CFR 122.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... apparatus, inflatable life jackets, and inflated rescue boats. 122.730 Section 122.730 Shipping COAST GUARD..., inflatable life jackets, and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant... other standard specified by the Commandant. (e) Repair and maintenance of inflated rescue boats must be...

  14. 46 CFR 185.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... apparatus, inflatable life jackets, and inflated rescue boats. 185.730 Section 185.730 Shipping COAST GUARD... liferafts, inflatable buoyant apparatus, inflatable life jackets, and inflated rescue boats. (a) An... inflated rescue boats must be in accordance with the manufacturer's instructions. All repairs must be made...

  15. 46 CFR 122.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... apparatus, inflatable life jackets, and inflated rescue boats. 122.730 Section 122.730 Shipping COAST GUARD..., inflatable life jackets, and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant... other standard specified by the Commandant. (e) Repair and maintenance of inflated rescue boats must be...

  16. 46 CFR 185.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... apparatus, inflatable life jackets, and inflated rescue boats. 185.730 Section 185.730 Shipping COAST GUARD... liferafts, inflatable buoyant apparatus, inflatable life jackets, and inflated rescue boats. (a) An... inflated rescue boats must be in accordance with the manufacturer's instructions. All repairs must be made...

  17. 46 CFR 122.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... apparatus, inflatable life jackets, and inflated rescue boats. 122.730 Section 122.730 Shipping COAST GUARD..., inflatable life jackets, and inflated rescue boats. (a) An inflatable liferaft or inflatable buoyant... other standard specified by the Commandant. (e) Repair and maintenance of inflated rescue boats must be...

  18. 46 CFR 185.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... apparatus, inflatable life jackets, and inflated rescue boats. 185.730 Section 185.730 Shipping COAST GUARD... liferafts, inflatable buoyant apparatus, inflatable life jackets, and inflated rescue boats. (a) An... inflated rescue boats must be in accordance with the manufacturer's instructions. All repairs must be made...

  19. 46 CFR 185.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... inflatable liferaft or inflatable buoyant apparatus must be serviced at a facility specifically approved by... apparatus, inflatable life jackets, and inflated rescue boats. 185.730 Section 185.730 Shipping COAST GUARD... Operational Readiness, Maintenance, and Inspection of Lifesaving Equipment § 185.730 Servicing of inflatable...

  20. 46 CFR 185.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... inflatable liferaft or inflatable buoyant apparatus must be serviced at a facility specifically approved by... apparatus, inflatable life jackets, and inflated rescue boats. 185.730 Section 185.730 Shipping COAST GUARD... Operational Readiness, Maintenance, and Inspection of Lifesaving Equipment § 185.730 Servicing of inflatable...

  1. 46 CFR 122.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Inspection of Lifesaving Equipment § 122.730 Servicing of inflatable liferafts, inflatable buoyant apparatus... apparatus must be serviced at a facility specifically approved by the Commandant for the particular brand... apparatus, inflatable life jackets, and inflated rescue boats. 122.730 Section 122.730 Shipping COAST GUARD...

  2. 46 CFR 122.730 - Servicing of inflatable liferafts, inflatable buoyant apparatus, inflatable life jackets, and...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Inspection of Lifesaving Equipment § 122.730 Servicing of inflatable liferafts, inflatable buoyant apparatus... apparatus must be serviced at a facility specifically approved by the Commandant for the particular brand... apparatus, inflatable life jackets, and inflated rescue boats. 122.730 Section 122.730 Shipping COAST GUARD...

  3. 46 CFR 160.051-7 - Design and performance of Coastal Service inflatable liferafts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... diameter of the buoyancy chambers is greater than 500 mm (19.5 in). (g) Stability (IMO LSA Code, chapter IV/4.2.5). Each Coastal Service inflatable liferaft must either meet the stability criteria in § 160.151-17(a) or be fitted with water-containing stability pockets meeting the following requirements: (1...

  4. 46 CFR 160.151-29 - Additional approval tests for SOLAS A and SOLAS B inflatable liferafts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... Resolution A.689(17): (a) Test of filling time for stability appendages. A representative sample of each type... 46 Shipping 6 2011-10-01 2011-10-01 false Additional approval tests for SOLAS A and SOLAS B... Inflatable Liferafts (SOLAS) § 160.151-29 Additional approval tests for SOLAS A and SOLAS B inflatable...

  5. 46 CFR 160.151-17 - Additional requirements for design and performance of SOLAS A and SOLAS B inflatable liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... the following requirements: (i) The total volume of the appendages must not be less than 220 liters (7.77 ft3) for liferafts approved to accommodate up to 10 persons. The volume of an appendage is... deducting the volume of any objects inside the appendage. No opening designed to close as water is forced...

  6. Big data analytics in hyperspectral imaging for detection of microbial colonies on agar plates (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Yoon, Seung-Chul; Park, Bosoon; Lawrence, Kurt C.

    2017-05-01

    Various types of optical imaging techniques measuring light reflectivity and scattering can detect microbial colonies of foodborne pathogens on agar plates. Until recently, these techniques were developed to provide solutions for hypothesis-driven studies, which focused on developing tools and batch/offline machine learning methods with well defined sets of data. These have relatively high accuracy and rapid response time because the tools and methods are often optimized for the collected data. However, they often need to be retrained or recalibrated when new untrained data and/or features are added. A big-data driven technique is more suitable for online learning of new/ambiguous samples and for mining unknown or hidden features. Although big data research in hyperspectral imaging is emerging in remote sensing and many tools and methods have been developed so far in many other applications such as bioinformatics, the tools and methods still need to be evaluated and adjusted in applications where the conventional batch machine learning algorithms were dominant. The primary objective of this study is to evaluate appropriate big data analytic tools and methods for online learning and mining of foodborne pathogens on agar plates. After the tools and methods are successfully identified, they will be applied to rapidly search big color and hyperspectral image data of microbial colonies collected over the past 5 years in house and find the most probable colony or a group of colonies in the collected big data. The meta-data, such as collection time and any unstructured data (e.g. comments), will also be analyzed and presented with output results. The expected results will be novel, big data-driven technology to correctly detect and recognize microbial colonies of various foodborne pathogens on agar plates.

  7. CLARA: CLAS12 Reconstruction and Analysis Framework

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Gyurjyan, Vardan; Matta, Sebastian Mancilla; Oyarzun, Ricardo

    2016-11-01

    In this paper we present SOA based CLAS12 event Reconstruction and Analyses (CLARA) framework. CLARA design focus is on two main traits: real-time data stream processing, and service-oriented architecture (SOA) in a flow based programming (FBP) paradigm. Data driven and data centric architecture of CLARA presents an environment for developing agile, elastic, multilingual data processing applications. The CLARA framework presents solutions capable of processing large volumes of data interactively and substantially faster than batch systems.

  8. Evaluation of intensity drift correction strategies using MetaboDrift, a normalization tool for multi-batch metabolomics data.

    PubMed

    Thonusin, Chanisa; IglayReger, Heidi B; Soni, Tanu; Rothberg, Amy E; Burant, Charles F; Evans, Charles R

    2017-11-10

    In recent years, mass spectrometry-based metabolomics has increasingly been applied to large-scale epidemiological studies of human subjects. However, the successful use of metabolomics in this context is subject to the challenge of detecting biologically significant effects despite substantial intensity drift that often occurs when data are acquired over a long period or in multiple batches. Numerous computational strategies and software tools have been developed to aid in correcting for intensity drift in metabolomics data, but most of these techniques are implemented using command-line driven software and custom scripts which are not accessible to all end users of metabolomics data. Further, it has not yet become routine practice to assess the quantitative accuracy of drift correction against techniques which enable true absolute quantitation such as isotope dilution mass spectrometry. We developed an Excel-based tool, MetaboDrift, to visually evaluate and correct for intensity drift in a multi-batch liquid chromatography - mass spectrometry (LC-MS) metabolomics dataset. The tool enables drift correction based on either quality control (QC) samples analyzed throughout the batches or using QC-sample independent methods. We applied MetaboDrift to an original set of clinical metabolomics data from a mixed-meal tolerance test (MMTT). The performance of the method was evaluated for multiple classes of metabolites by comparison with normalization using isotope-labeled internal standards. QC sample-based intensity drift correction significantly improved correlation with IS-normalized data, and resulted in detection of additional metabolites with significant physiological response to the MMTT. The relative merits of different QC-sample curve fitting strategies are discussed in the context of batch size and drift pattern complexity. Our drift correction tool offers a practical, simplified approach to drift correction and batch combination in large metabolomics studies. Copyright © 2017 Elsevier B.V. All rights reserved.

  9. Options for liferaft entry after helicopter ditching.

    PubMed

    Brooks, C J; Potter, P L; De Lange, D; Baranski, J V; Anderson, J

    1998-08-01

    Dry and wet evacuations were conducted by 24 male and 19 female subjects from the Nutec Super Puma Simulator into two different types of aviation liferaft. Dry evacuation on the windward side is the method of choice. The non-canopy raft is subjectively and objectively easier to enter both from the helicopter and the sea. The non-canopy raft is the raft of choice, the canopy raft needs a redesign to ensure that it always inflates the correct way and both rafts need a redesign of the painter anchor point. Aircrew should have special training in open water after traditional pool training. A helicopter ditching survival compass has been developed for training all who fly over water for a living.

  10. Workload-Driven Design and Evaluation of Large-Scale Data-Centric Systems

    DTIC Science & Technology

    2012-05-09

    in the batch zone in and out of a low-power state, e.g., sending a “ hibernate ” command via ssh and using Wake-on-LAN or related technologies [85]. If...parameter values for experiments with stand-alone jobs. The mapred.child.java.opts parameter sets the maximum virtual memory of the Java child pro- cesses

  11. Data-driven model reference control of MIMO vertical tank systems with model-free VRFT and Q-Learning.

    PubMed

    Radac, Mircea-Bogdan; Precup, Radu-Emil; Roman, Raul-Cristian

    2018-02-01

    This paper proposes a combined Virtual Reference Feedback Tuning-Q-learning model-free control approach, which tunes nonlinear static state feedback controllers to achieve output model reference tracking in an optimal control framework. The novel iterative Batch Fitted Q-learning strategy uses two neural networks to represent the value function (critic) and the controller (actor), and it is referred to as a mixed Virtual Reference Feedback Tuning-Batch Fitted Q-learning approach. Learning convergence of the Q-learning schemes generally depends, among other settings, on the efficient exploration of the state-action space. Handcrafting test signals for efficient exploration is difficult even for input-output stable unknown processes. Virtual Reference Feedback Tuning can ensure an initial stabilizing controller to be learned from few input-output data and it can be next used to collect substantially more input-state data in a controlled mode, in a constrained environment, by compensating the process dynamics. This data is used to learn significantly superior nonlinear state feedback neural networks controllers for model reference tracking, using the proposed Batch Fitted Q-learning iterative tuning strategy, motivating the original combination of the two techniques. The mixed Virtual Reference Feedback Tuning-Batch Fitted Q-learning approach is experimentally validated for water level control of a multi input-multi output nonlinear constrained coupled two-tank system. Discussions on the observed control behavior are offered. Copyright © 2018 ISA. Published by Elsevier Ltd. All rights reserved.

  12. Unifying cancer and normal RNA sequencing data from different sources

    PubMed Central

    Wang, Qingguo; Armenia, Joshua; Zhang, Chao; Penson, Alexander V.; Reznik, Ed; Zhang, Liguo; Minet, Thais; Ochoa, Angelica; Gross, Benjamin E.; Iacobuzio-Donahue, Christine A.; Betel, Doron; Taylor, Barry S.; Gao, Jianjiong; Schultz, Nikolaus

    2018-01-01

    Driven by the recent advances of next generation sequencing (NGS) technologies and an urgent need to decode complex human diseases, a multitude of large-scale studies were conducted recently that have resulted in an unprecedented volume of whole transcriptome sequencing (RNA-seq) data, such as the Genotype Tissue Expression project (GTEx) and The Cancer Genome Atlas (TCGA). While these data offer new opportunities to identify the mechanisms underlying disease, the comparison of data from different sources remains challenging, due to differences in sample and data processing. Here, we developed a pipeline that processes and unifies RNA-seq data from different studies, which includes uniform realignment, gene expression quantification, and batch effect removal. We find that uniform alignment and quantification is not sufficient when combining RNA-seq data from different sources and that the removal of other batch effects is essential to facilitate data comparison. We have processed data from GTEx and TCGA and successfully corrected for study-specific biases, enabling comparative analysis between TCGA and GTEx. The normalized datasets are available for download on figshare. PMID:29664468

  13. Missing data and technical variability in single-cell RNA-sequencing experiments.

    PubMed

    Hicks, Stephanie C; Townes, F William; Teng, Mingxiang; Irizarry, Rafael A

    2017-11-06

    Until recently, high-throughput gene expression technology, such as RNA-Sequencing (RNA-seq) required hundreds of thousands of cells to produce reliable measurements. Recent technical advances permit genome-wide gene expression measurement at the single-cell level. Single-cell RNA-Seq (scRNA-seq) is the most widely used and numerous publications are based on data produced with this technology. However, RNA-seq and scRNA-seq data are markedly different. In particular, unlike RNA-seq, the majority of reported expression levels in scRNA-seq are zeros, which could be either biologically-driven, genes not expressing RNA at the time of measurement, or technically-driven, genes expressing RNA, but not at a sufficient level to be detected by sequencing technology. Another difference is that the proportion of genes reporting the expression level to be zero varies substantially across single cells compared to RNA-seq samples. However, it remains unclear to what extent this cell-to-cell variation is being driven by technical rather than biological variation. Furthermore, while systematic errors, including batch effects, have been widely reported as a major challenge in high-throughput technologies, these issues have received minimal attention in published studies based on scRNA-seq technology. Here, we use an assessment experiment to examine data from published studies and demonstrate that systematic errors can explain a substantial percentage of observed cell-to-cell expression variability. Specifically, we present evidence that some of these reported zeros are driven by technical variation by demonstrating that scRNA-seq produces more zeros than expected and that this bias is greater for lower expressed genes. In addition, this missing data problem is exacerbated by the fact that this technical variation varies cell-to-cell. Then, we show how this technical cell-to-cell variability can be confused with novel biological results. Finally, we demonstrate and discuss how batch-effects and confounded experiments can intensify the problem. © The Author 2017. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  14. 46 CFR 160.151-57 - Servicing procedure.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... its weight loss exceeds five percent of the weight of the charge, it must be recharged. (c) When an... in the buoyancy tubes is audible, accompanied by pressure loss, condemn the liferaft. If it is not...

  15. Computational Testing for Automated Preprocessing 2: Practical Demonstration of a System for Scientific Data-Processing Workflow Management for High-Volume EEG

    PubMed Central

    Cowley, Benjamin U.; Korpela, Jussi

    2018-01-01

    Existing tools for the preprocessing of EEG data provide a large choice of methods to suitably prepare and analyse a given dataset. Yet it remains a challenge for the average user to integrate methods for batch processing of the increasingly large datasets of modern research, and compare methods to choose an optimal approach across the many possible parameter configurations. Additionally, many tools still require a high degree of manual decision making for, e.g., the classification of artifacts in channels, epochs or segments. This introduces extra subjectivity, is slow, and is not reproducible. Batching and well-designed automation can help to regularize EEG preprocessing, and thus reduce human effort, subjectivity, and consequent error. The Computational Testing for Automated Preprocessing (CTAP) toolbox facilitates: (i) batch processing that is easy for experts and novices alike; (ii) testing and comparison of preprocessing methods. Here we demonstrate the application of CTAP to high-resolution EEG data in three modes of use. First, a linear processing pipeline with mostly default parameters illustrates ease-of-use for naive users. Second, a branching pipeline illustrates CTAP's support for comparison of competing methods. Third, a pipeline with built-in parameter-sweeping illustrates CTAP's capability to support data-driven method parameterization. CTAP extends the existing functions and data structure from the well-known EEGLAB toolbox, based on Matlab, and produces extensive quality control outputs. CTAP is available under MIT open-source licence from https://github.com/bwrc/ctap. PMID:29692705

  16. Computational Testing for Automated Preprocessing 2: Practical Demonstration of a System for Scientific Data-Processing Workflow Management for High-Volume EEG.

    PubMed

    Cowley, Benjamin U; Korpela, Jussi

    2018-01-01

    Existing tools for the preprocessing of EEG data provide a large choice of methods to suitably prepare and analyse a given dataset. Yet it remains a challenge for the average user to integrate methods for batch processing of the increasingly large datasets of modern research, and compare methods to choose an optimal approach across the many possible parameter configurations. Additionally, many tools still require a high degree of manual decision making for, e.g., the classification of artifacts in channels, epochs or segments. This introduces extra subjectivity, is slow, and is not reproducible. Batching and well-designed automation can help to regularize EEG preprocessing, and thus reduce human effort, subjectivity, and consequent error. The Computational Testing for Automated Preprocessing (CTAP) toolbox facilitates: (i) batch processing that is easy for experts and novices alike; (ii) testing and comparison of preprocessing methods. Here we demonstrate the application of CTAP to high-resolution EEG data in three modes of use. First, a linear processing pipeline with mostly default parameters illustrates ease-of-use for naive users. Second, a branching pipeline illustrates CTAP's support for comparison of competing methods. Third, a pipeline with built-in parameter-sweeping illustrates CTAP's capability to support data-driven method parameterization. CTAP extends the existing functions and data structure from the well-known EEGLAB toolbox, based on Matlab, and produces extensive quality control outputs. CTAP is available under MIT open-source licence from https://github.com/bwrc/ctap.

  17. 46 CFR 160.026-7 - Procedure for approval.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... lifeboats and liferafts on merchant vessels is approved only by the Commandant, U.S. Coast Guard. (b) Pre... required tests in accordance with § 160.026-5 performed by an independent laboratory accepted by the Coast...

  18. 46 CFR 12.10-5 - Examination and demonstration of ability.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... liferafts, the handling of lifeboats under oars and sails, including questions relative to the proper... out the orders incident to launching lifeboats, and the use of the boat's sail, and to row. (d) Each...

  19. 46 CFR 12.10-5 - Examination and demonstration of ability.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... liferafts, the handling of lifeboats under oars and sails, including questions relative to the proper... out the orders incident to launching lifeboats, and the use of the boat's sail, and to row. (d) Each...

  20. 46 CFR 131.860 - Rigid liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ..., over each entrance to each raft, in letters and numbers at least 102 millimeters (4 inches) high and in a color contrasting to that of the raft. This number must— (1) Be the number of persons the rigid...

  1. 46 CFR 160.010-2 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Buoyant Apparatus for Merchant Vessels § 160.010-2 Definitions. Buoyant apparatus. Buoyant apparatus is flotation equipment (other than lifeboats, liferafts, and personal...

  2. 46 CFR 169.849 - Posting placards containing instructions for launching and inflating inflatable liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Inspections § 169.849 Posting placards containing instructions for launching and inflating inflatable... accessible to the ship's company and guests approved placards containing instructions for launching and... determined by the Officer in Charge, Marine Inspection. ...

  3. Effects of region, demography, and protection from fishing on batch fecundity of common coral trout ( Plectropomus leopardus)

    NASA Astrophysics Data System (ADS)

    Carter, Alex B.; Davies, Campbell R.; Mapstone, Bruce D.; Russ, Garry R.; Tobin, Andrew J.; Williams, Ashley J.

    2014-09-01

    Batch fecundity of female Plectropomus leopardus, a coral reef fish targeted by commercial and recreational fishing, was compared between reefs open to fishing and reefs within no-take marine reserves within three regions of the Great Barrier Reef (GBR), Australia. Length, weight, and age had positive effects on batch fecundity of spawners from northern and central reefs but negligible effects on spawners from southern reefs. Females were least fecund for a given length, weight, and age in the southern GBR. Batch fecundity of a 500-mm fork length female was 430 % greater on central reefs and 207 % greater on northern reefs than on southern reefs. The effects of length and age on batch fecundity did not differ significantly between reserve and fished reefs in any region, but weight-specific fecundity was 100 % greater for large 2.0 kg females on reserve reefs compared with fished reefs in the central GBR. We hypothesize that regional variation in batch fecundity is likely driven by water temperature and prey availability. Significant regional variation in batch fecundity highlights the need for understanding spatial variation in reproductive output where single conservation or fishery management strategies cover large, potentially diverse, spatial scales.

  4. 46 CFR 160.042-3 - Construction.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 6 2014-10-01 2014-10-01 false Construction. 160.042-3 Section 160.042-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Skids, Liferaft, for Merchant Vessels § 160.042-3 Construction...

  5. 46 CFR 160.042-3 - Construction.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Construction. 160.042-3 Section 160.042-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Skids, Liferaft, for Merchant Vessels § 160.042-3 Construction...

  6. 46 CFR 160.042-3 - Construction.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 6 2012-10-01 2012-10-01 false Construction. 160.042-3 Section 160.042-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Skids, Liferaft, for Merchant Vessels § 160.042-3 Construction...

  7. 46 CFR 160.042-3 - Construction.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 6 2013-10-01 2013-10-01 false Construction. 160.042-3 Section 160.042-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Skids, Liferaft, for Merchant Vessels § 160.042-3 Construction...

  8. 46 CFR 160.054-3 - Construction.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Construction. 160.054-3 Section 160.054-3 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-3...

  9. 77 FR 9859 - Lifesaving Equipment: Production Testing and Harmonization With International Standards

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-21

    ... inflatable buoyant apparatuses may be certified using either occupant weight standard. Likewise, equipment...-AB46 Lifesaving Equipment: Production Testing and Harmonization With International Standards AGENCY... lifesaving equipment to harmonize Coast Guard regulations for inflatable liferafts and inflatable buoyant...

  10. 46 CFR 169.525 - General.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... Firefighting Equipment Equipment for Primary Lifesaving Apparatus § 169.525 General. (a) Equipment for primary lifesaving apparatus must kept in good condition. (b) Lifeboats, inflatable liferafts and lifefloats must be... apparatus. (d) Loose equipment, except boathooks in lifeboats, must be securely attached to the lifesaving...

  11. 46 CFR 115.808 - Lifesaving.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) Inspection of each life jacket, work vest, and marine buoyant device; (3) If used, inspection of the... of each inflatable liferaft, inflatable buoyant apparatus, and inflatable life jacket to determine... managing operator shall destroy, in the presence of the marine inspector, each life jacket, other personal...

  12. 46 CFR 160.151-43 - Conditions at servicing facilities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Conditions at servicing facilities. 160.151-43 Section 160.151-43 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts (SOLAS) § 160.151...

  13. 46 CFR 199.180 - Training and drills.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... survival craft engine and accessories; (xiii) The recovery of survival craft and rescue boats, including... and operating the lifeboat engine; and (vii) Operating davits used for launching the liferafts. (2) Abandon-ship drills should also include conducting a mock search and rescue of passengers or special...

  14. 46 CFR 199.180 - Training and drills.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... survival craft engine and accessories; (xiii) The recovery of survival craft and rescue boats, including... and operating the lifeboat engine; and (vii) Operating davits used for launching the liferafts. (2) Abandon-ship drills should also include conducting a mock search and rescue of passengers or special...

  15. 46 CFR 199.180 - Training and drills.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... survival craft engine and accessories; (xiii) The recovery of survival craft and rescue boats, including... and operating the lifeboat engine; and (vii) Operating davits used for launching the liferafts. (2) Abandon-ship drills should also include conducting a mock search and rescue of passengers or special...

  16. 46 CFR 169.507 - Responsibility of master.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Responsibility of master. 169.507 Section 169.507... Lifesaving and Firefighting Equipment Lifesaving Equipment-General § 169.507 Responsibility of master. The master or operator shall ensure that the lifeboats, liferafts, davits, falls, personal flotation devices...

  17. 46 CFR 169.507 - Responsibility of master.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Responsibility of master. 169.507 Section 169.507... Lifesaving and Firefighting Equipment Lifesaving Equipment-General § 169.507 Responsibility of master. The master or operator shall ensure that the lifeboats, liferafts, davits, falls, personal flotation devices...

  18. 46 CFR 169.507 - Responsibility of master.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Responsibility of master. 169.507 Section 169.507... Lifesaving and Firefighting Equipment Lifesaving Equipment-General § 169.507 Responsibility of master. The master or operator shall ensure that the lifeboats, liferafts, davits, falls, personal flotation devices...

  19. 46 CFR 169.507 - Responsibility of master.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Responsibility of master. 169.507 Section 169.507... Lifesaving and Firefighting Equipment Lifesaving Equipment-General § 169.507 Responsibility of master. The master or operator shall ensure that the lifeboats, liferafts, davits, falls, personal flotation devices...

  20. 46 CFR 169.507 - Responsibility of master.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Responsibility of master. 169.507 Section 169.507... Lifesaving and Firefighting Equipment Lifesaving Equipment-General § 169.507 Responsibility of master. The master or operator shall ensure that the lifeboats, liferafts, davits, falls, personal flotation devices...

  1. 46 CFR 199.180 - Training and drills.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... survival craft engine and accessories; (xiii) The recovery of survival craft and rescue boats, including... and operating the lifeboat engine; and (vii) Operating davits used for launching the liferafts. (2) Abandon-ship drills should also include conducting a mock search and rescue of passengers or special...

  2. 46 CFR 199.180 - Training and drills.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... survival craft engine and accessories; (xiii) The recovery of survival craft and rescue boats, including... and operating the lifeboat engine; and (vii) Operating davits used for launching the liferafts. (2) Abandon-ship drills should also include conducting a mock search and rescue of passengers or special...

  3. 46 CFR 160.151-41 - Approval of servicing facilities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Approval of servicing facilities. 160.151-41 Section 160.151-41 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts (SOLAS) § 160.151-41...

  4. 46 CFR 160.151-47 - Requirements for owners or operators of servicing facilities.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... facilities. 160.151-47 Section 160.151-47 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts (SOLAS) § 160.151-47 Requirements for owners or operators of servicing facilities. To maintain...

  5. 46 CFR 91.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... of liferafts and buoyant apparatus. (h) Crew's accommodations. (1) Arrangement plans showing... Drains Penetrating Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The...) Subdivision and stability. Plans and calculations as required by Subchapter S of this chapter. (d) Fire...

  6. 46 CFR 91.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... of liferafts and buoyant apparatus. (h) Crew's accommodations. (1) Arrangement plans showing... Drains Penetrating Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The...) Subdivision and stability. Plans and calculations as required by Subchapter S of this chapter. (d) Fire...

  7. 46 CFR 91.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... of liferafts and buoyant apparatus. (h) Crew's accommodations. (1) Arrangement plans showing... Drains Penetrating Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The...) Subdivision and stability. Plans and calculations as required by Subchapter S of this chapter. (d) Fire...

  8. 46 CFR 160.054-5 - Inspections and tests.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 6 2013-10-01 2013-10-01 false Inspections and tests. 160.054-5 Section 160.054-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-5...

  9. 46 CFR 160.054-5 - Inspections and tests.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 6 2012-10-01 2012-10-01 false Inspections and tests. 160.054-5 Section 160.054-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-5...

  10. 46 CFR 160.054-5 - Inspections and tests.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 6 2014-10-01 2014-10-01 false Inspections and tests. 160.054-5 Section 160.054-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-5...

  11. 46 CFR 160.054-5 - Inspections and tests.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Inspections and tests. 160.054-5 Section 160.054-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-5...

  12. 46 CFR 169.515 - Number required.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Number required. 169.515 Section 169.515 Shipping COAST... and Firefighting Equipment Primary Lifesaving Equipment § 169.515 Number required. (a) Except as... additional inflatable liferafts to accommodate 25% of the persons on board or the number of persons...

  13. 46 CFR 169.515 - Number required.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Number required. 169.515 Section 169.515 Shipping COAST... and Firefighting Equipment Primary Lifesaving Equipment § 169.515 Number required. (a) Except as... additional inflatable liferafts to accommodate 25% of the persons on board or the number of persons...

  14. 46 CFR 169.515 - Number required.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Number required. 169.515 Section 169.515 Shipping COAST... and Firefighting Equipment Primary Lifesaving Equipment § 169.515 Number required. (a) Except as... additional inflatable liferafts to accommodate 25% of the persons on board or the number of persons...

  15. 46 CFR 169.515 - Number required.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Number required. 169.515 Section 169.515 Shipping COAST... and Firefighting Equipment Primary Lifesaving Equipment § 169.515 Number required. (a) Except as... additional inflatable liferafts to accommodate 25% of the persons on board or the number of persons...

  16. 46 CFR 169.515 - Number required.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Number required. 169.515 Section 169.515 Shipping COAST... and Firefighting Equipment Primary Lifesaving Equipment § 169.515 Number required. (a) Except as... additional inflatable liferafts to accommodate 25% of the persons on board or the number of persons...

  17. 46 CFR 160.054-5 - Inspections and tests.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Inspections and tests. 160.054-5 Section 160.054-5 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-5...

  18. 46 CFR 160.151-39 - Training of servicing technicians.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Training of servicing technicians. 160.151-39 Section...-39 Training of servicing technicians. (a) The training program for certification of servicing technicians must include— (1) Training and practice in packing an inflatable liferaft, repairing buoyancy...

  19. In vitro growth of Curcuma longa L. in response to five mineral elements and plant density in fed-batch culture systems.

    PubMed

    El-Hawaz, Rabia F; Bridges, William C; Adelberg, Jeffrey W

    2015-01-01

    Plant density was varied with P, Ca, Mg, and KNO3 in a multifactor experiment to improve Curcuma longa L. micropropagation, biomass and microrhizome development in fed-batch liquid culture. The experiment had two paired D-optimal designs, testing sucrose fed-batch and nutrient sucrose fed-batch techniques. When sucrose became depleted, volume was restored to 5% m/v sucrose in 200 ml of modified liquid MS medium by adding sucrose solutions. Similarly, nutrient sucrose fed-batch was restored to set points with double concentration of treatments' macronutrient and MS micronutrient solutions, along with sucrose solutions. Changes in the amounts of water and sucrose supplementations were driven by the interaction of P and KNO3 concentrations. Increasing P from 1.25 to 6.25 mM increased both multiplication and biomass. The multiplication ratio was greatest in the nutrient sucrose fed-batch technique with the highest level of P, 6 buds/vessel, and the lowest level of Ca and KNO3. The highest density (18 buds/vessel) produced the highest fresh biomass at the highest concentrations of KNO3 and P with nutrient sucrose fed-batch, and moderate Ca and Mg concentrations. However, maximal rhizome dry biomass required highest P, sucrose fed-batch, and a moderate plant density. Different media formulations and fed-batch techniques were identified to maximize the propagation and storage organ responses. A single experimental design was used to optimize these dual purposes.

  20. In Vitro Growth of Curcuma longa L. in Response to Five Mineral Elements and Plant Density in Fed-Batch Culture Systems

    PubMed Central

    El-Hawaz, Rabia F.; Bridges, William C.; Adelberg, Jeffrey W.

    2015-01-01

    Plant density was varied with P, Ca, Mg, and KNO3 in a multifactor experiment to improve Curcuma longa L. micropropagation, biomass and microrhizome development in fed-batch liquid culture. The experiment had two paired D-optimal designs, testing sucrose fed-batch and nutrient sucrose fed-batch techniques. When sucrose became depleted, volume was restored to 5% m/v sucrose in 200 ml of modified liquid MS medium by adding sucrose solutions. Similarly, nutrient sucrose fed-batch was restored to set points with double concentration of treatments’ macronutrient and MS micronutrient solutions, along with sucrose solutions. Changes in the amounts of water and sucrose supplementations were driven by the interaction of P and KNO3 concentrations. Increasing P from 1.25 to 6.25 mM increased both multiplication and biomass. The multiplication ratio was greatest in the nutrient sucrose fed-batch technique with the highest level of P, 6 buds/vessel, and the lowest level of Ca and KNO3. The highest density (18 buds/vessel) produced the highest fresh biomass at the highest concentrations of KNO3 and P with nutrient sucrose fed-batch, and moderate Ca and Mg concentrations. However, maximal rhizome dry biomass required highest P, sucrose fed-batch, and a moderate plant density. Different media formulations and fed-batch techniques were identified to maximize the propagation and storage organ responses. A single experimental design was used to optimize these dual purposes. PMID:25830292

  1. 33 CFR 150.507 - How must the operator service inflatable lifesaving appliances?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... inflatable lifesaving appliances? 150.507 Section 150.507 Navigation and Navigable Waters COAST GUARD... Specialty Equipment Inflatable Lifesaving Appliances § 150.507 How must the operator service inflatable lifesaving appliances? (a) The operator must service each inflatable liferaft according to 46 CFR subpart 160...

  2. 46 CFR 28.130 - Survival craft equipment.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... survival craft equipment must be of good quality, effective for the purpose it is intended to serve, and... “Limited Service”); or (3) SOLAS A Pack (formerly “Ocean Service”). (c) Each life float and buoyant... survival craft other than inflatable liferafts, life floats, inflatable buoyant apparatus, or buoyant...

  3. 46 CFR 28.130 - Survival craft equipment.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... survival craft equipment must be of good quality, effective for the purpose it is intended to serve, and... “Limited Service”); or (3) SOLAS A Pack (formerly “Ocean Service”). (c) Each life float and buoyant... survival craft other than inflatable liferafts, life floats, inflatable buoyant apparatus, or buoyant...

  4. 46 CFR 28.130 - Survival craft equipment.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... survival craft equipment must be of good quality, effective for the purpose it is intended to serve, and... “Limited Service”); or (3) SOLAS A Pack (formerly “Ocean Service”). (c) Each life float and buoyant... survival craft other than inflatable liferafts, life floats, inflatable buoyant apparatus, or buoyant...

  5. 46 CFR 28.130 - Survival craft equipment.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... survival craft equipment must be of good quality, effective for the purpose it is intended to serve, and... “Limited Service”); or (3) SOLAS A Pack (formerly “Ocean Service”). (c) Each life float and buoyant... survival craft other than inflatable liferafts, life floats, inflatable buoyant apparatus, or buoyant...

  6. 46 CFR 28.130 - Survival craft equipment.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... survival craft equipment must be of good quality, effective for the purpose it is intended to serve, and... “Limited Service”); or (3) SOLAS A Pack (formerly “Ocean Service”). (c) Each life float and buoyant... survival craft other than inflatable liferafts, life floats, inflatable buoyant apparatus, or buoyant...

  7. 46 CFR 160.054-4 - Contents.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 6 2014-10-01 2014-10-01 false Contents. 160.054-4 Section 160.054-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-4 Contents. (a) Individual cartons. Cartons and their...

  8. 46 CFR 160.054-4 - Contents.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 6 2013-10-01 2013-10-01 false Contents. 160.054-4 Section 160.054-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-4 Contents. (a) Individual cartons. Cartons and their...

  9. 46 CFR 160.054-4 - Contents.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Contents. 160.054-4 Section 160.054-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-4 Contents. (a) Individual cartons. Cartons and their...

  10. 46 CFR 160.054-4 - Contents.

    Code of Federal Regulations, 2010 CFR

    2012-10-01

    ... 46 Shipping 6 2012-10-01 2012-10-01 false Contents. 160.054-4 Section 160.054-4 Shipping COAST...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-4 Contents. (a) Individual cartons. Cartons and their contents shall be the type indicated in Specification...

  11. 46 CFR 160.054-4 - Contents.

    Code of Federal Regulations, 2010 CFR

    2005-10-01

    ... 46 Shipping 6 2005-10-01 2004-10-01 true Contents. 160.054-4 Section 160.054-4 Shipping Coast...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-4 Contents. (a) Individual cartons. Cartons and their contents shall be the type indicated in Specification...

  12. 46 CFR 199.245 - Survival craft embarkation and launching arrangements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Survival craft embarkation and launching arrangements... Passenger Vessels § 199.245 Survival craft embarkation and launching arrangements. (a) Each davit-launched liferaft must be arranged to be rapidly boarded by its full complement of persons. (b) All survival craft...

  13. 46 CFR 199.201 - Survival craft.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Survival craft. 199.201 Section 199.201 Shipping COAST... craft. (a) Each survival craft must be approved and equipped as follows: (1) Each lifeboat must be... addition to the survival craft required in paragraph (b)(1) of this section, additional liferafts must be...

  14. 46 CFR 199.261 - Survival craft.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... a SOLAS A pack. (3) Each rigid liferaft must be approved under approval series 160.118 and be... or more. (5) Each marine evacuation system must be approved under approval series 160.175. (b) Each... least one side of the vessel being served by launching appliances or marine evacuation systems. (d...

  15. 46 CFR 199.261 - Survival craft.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... a SOLAS A pack. (3) Each rigid liferaft must be approved under approval series 160.118 and be... or more. (5) Each marine evacuation system must be approved under approval series 160.175. (b) Each... least one side of the vessel being served by launching appliances or marine evacuation systems. (d...

  16. 46 CFR 199.261 - Survival craft.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... a SOLAS A pack. (3) Each rigid liferaft must be approved under approval series 160.118 and be... or more. (5) Each marine evacuation system must be approved under approval series 160.175. (b) Each... least one side of the vessel being served by launching appliances or marine evacuation systems. (d...

  17. 46 CFR 199.261 - Survival craft.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... a SOLAS A pack. (3) Each rigid liferaft must be approved under approval series 160.118 and be... or more. (5) Each marine evacuation system must be approved under approval series 160.175. (b) Each... least one side of the vessel being served by launching appliances or marine evacuation systems. (d...

  18. 46 CFR 160.054-4 - Contents.

    Code of Federal Regulations, 2010 CFR

    2007-10-01

    ... 46 Shipping 6 2007-10-01 2007-10-01 false Contents. 160.054-4 Section 160.054-4 Shipping Coast Guard, Department of Homeland Security (Continued) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-4 Contents. (a) Individual cartons. Cartons and their...

  19. 46 CFR 91.55-5 - Plans and specifications required for new construction.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    .... (ii) Arrangement of davits. (iii) Location and stowage of liferafts and buoyant apparatus. (h) Crew's... Drains Penetrating Shell Plating. (13) *Arrangement of the cargo gear including a stress diagram. The...) Subdivision and stability. Plans and calculations as required by Subchapter S of this chapter. (d) Fire...

  20. 46 CFR 160.054-4 - Contents.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Contents. 160.054-4 Section 160.054-4 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-4 Contents. (a) Individual cartons. Cartons and their...

  1. 46 CFR 160.054-6 - Marking.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-6 Marking. (a) Each approved first-aid kit shall be plainly and permanently marked with the following... kit, the Coast Guard approval number, and the words “First-Aid Kit—For Inflatable Life Rafts”. If the...

  2. 46 CFR 160.054-1 - Applicable specification.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-1 Applicable specification. (a) Specification. The following specification of the issue in effect on the date first aid kits are manufactured forms a part of this subpart: (1) Coast Guard specification 160.041—Kits, First-Aid...

  3. 46 CFR 160.054-1 - Applicable specification.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-1 Applicable specification. (a) Specification. The following specification of the issue in effect on the date first aid kits are manufactured forms a part of this subpart: (1) Coast Guard specification 160.041—Kits, First-Aid...

  4. 46 CFR 160.054-1 - Applicable specification.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-1 Applicable specification. (a) Specification. The following specification of the issue in effect on the date first aid kits are manufactured forms a part of this subpart: (1) Coast Guard specification 160.041—Kits, First-Aid...

  5. 46 CFR 160.054-1 - Applicable specification.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-1 Applicable specification. (a) Specification. The following specification of the issue in effect on the date first aid kits are manufactured forms a part of this subpart: (1) Coast Guard specification 160.041—Kits, First-Aid...

  6. 46 CFR 160.054-6 - Marking.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-6 Marking. (a) Each approved first-aid kit shall be plainly and permanently marked with the following... kit, the Coast Guard approval number, and the words “First-Aid Kit—For Inflatable Life Rafts”. If the...

  7. 46 CFR 160.054-6 - Marking.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-6 Marking. (a) Each approved first-aid kit shall be plainly and permanently marked with the following... kit, the Coast Guard approval number, and the words “First-Aid Kit—For Inflatable Life Rafts”. If the...

  8. 46 CFR 160.054-6 - Marking.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-6 Marking. (a) Each approved first-aid kit shall be plainly and permanently marked with the following... kit, the Coast Guard approval number, and the words “First-Aid Kit—For Inflatable Life Rafts”. If the...

  9. 46 CFR 199.145 - Marine evacuation system launching arrangements.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 7 2012-10-01 2012-10-01 false Marine evacuation system launching arrangements. 199.145....145 Marine evacuation system launching arrangements. (a) Arrangements. Each marine evacuation system... from the marine evacuation system platform by a person either in the liferaft or on the platform; (4...

  10. 46 CFR 199.145 - Marine evacuation system launching arrangements.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 7 2013-10-01 2013-10-01 false Marine evacuation system launching arrangements. 199.145....145 Marine evacuation system launching arrangements. (a) Arrangements. Each marine evacuation system... from the marine evacuation system platform by a person either in the liferaft or on the platform; (4...

  11. 46 CFR 199.145 - Marine evacuation system launching arrangements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 7 2014-10-01 2014-10-01 false Marine evacuation system launching arrangements. 199.145....145 Marine evacuation system launching arrangements. (a) Arrangements. Each marine evacuation system... from the marine evacuation system platform by a person either in the liferaft or on the platform; (4...

  12. 46 CFR 199.145 - Marine evacuation system launching arrangements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Marine evacuation system launching arrangements. 199.145....145 Marine evacuation system launching arrangements. (a) Arrangements. Each marine evacuation system... from the marine evacuation system platform by a person either in the liferaft or on the platform; (4...

  13. 46 CFR 199.145 - Marine evacuation system launching arrangements.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 7 2011-10-01 2011-10-01 false Marine evacuation system launching arrangements. 199.145....145 Marine evacuation system launching arrangements. (a) Arrangements. Each marine evacuation system... from the marine evacuation system platform by a person either in the liferaft or on the platform; (4...

  14. 14 CFR 29.1415 - Ditching equipment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...) Emergency flotation and signaling equipment required by any operating rule of this chapter must meet the requirements of this section. (b) Each liferaft and each life preserver must be approved. In addition— (1... survival type emergency locator transmitter for use in one life raft. [Doc. No. 5084, 29 FR 16150, Dec. 3...

  15. 33 CFR 144.01-15 - Alternates for life floats.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., and character as required for lifeboats carried on vessels engaged in navigating bays, sounds, and... vessels navigating on bays, sounds, and lakes other than the Great Lakes. (d) Inflatable liferafts shall be approved by the Coast Guard under approval series 160.151. An approved “Limited Service” or “Ocean...

  16. 33 CFR 144.01-15 - Alternates for life floats.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., and character as required for lifeboats carried on vessels engaged in navigating bays, sounds, and... vessels navigating on bays, sounds, and lakes other than the Great Lakes. (d) Inflatable liferafts shall be approved by the Coast Guard under approval series 160.151. An approved “Limited Service” or “Ocean...

  17. 33 CFR 144.01-15 - Alternates for life floats.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., and character as required for lifeboats carried on vessels engaged in navigating bays, sounds, and... vessels navigating on bays, sounds, and lakes other than the Great Lakes. (d) Inflatable liferafts shall be approved by the Coast Guard under approval series 160.151. An approved “Limited Service” or “Ocean...

  18. 46 CFR 160.054-1 - Applicable specification.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-1 Applicable specification. (a) Specification. The following specification of the issue in effect on the date first aid kits are manufactured forms a part of this subpart: (1) Coast Guard specification 160.041—Kits, First-Aid...

  19. 46 CFR 160.054-6 - Marking.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-6 Marking. (a) Each approved first-aid kit shall be plainly and permanently marked with the following... kit, the Coast Guard approval number, and the words “First-Aid Kit—For Inflatable Life Rafts”. If the...

  20. 46 CFR 160.151-9 - Independent laboratory.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 6 2011-10-01 2011-10-01 false Independent laboratory. 160.151-9 Section 160.151-9...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts (SOLAS) § 160.151-9 Independent laboratory. Tests and inspections that this subpart requires to be conducted by an independent laboratory must be...

  1. 46 CFR 160.151-9 - Independent laboratory.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Independent laboratory. 160.151-9 Section 160.151-9...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts (SOLAS) § 160.151-9 Independent laboratory. Tests and inspections that this subpart requires to be conducted by an independent laboratory must be...

  2. Raw material variability of an active pharmaceutical ingredient and its relevance for processability in secondary continuous pharmaceutical manufacturing.

    PubMed

    Stauffer, F; Vanhoorne, V; Pilcer, G; Chavez, P-F; Rome, S; Schubert, M A; Aerts, L; De Beer, T

    2018-06-01

    Active Pharmaceutical Ingredients (API) raw material variability is not always thoroughly considered during pharmaceutical process development, mainly due to low quantities of drug substance available. However, synthesis, crystallization routes and production sites evolve during product development and product life cycle leading to changes in physical material attributes which can potentially affect their processability. Recent literature highlights the need for a global approach to understand the link between material synthesis, material variability, process and product quality. The study described in this article aims at explaining the raw material variability of an API using extensive material characterization on a restricted number of representative batches using multivariate data analysis. It is part of a larger investigation trying to link the API drug substance manufacturing process, the resulting physical API raw material attributes and the drug product continuous manufacturing process. Eight API batches produced using different synthetic routes, crystallization, drying, delumping processes and processing equipment were characterized, extensively. Seventeen properties from seven characterization techniques were retained for further analysis using Principal Component Analysis (PCA). Three principal components (PCs) were sufficient to explain 92.9% of the API raw material variability. The first PC was related to crystal length, agglomerate size and fraction, flowability and electrostatic charging. The second PC was driven by the span of the particle size distribution and the agglomerates strength. The third PC was related to surface energy. Additionally, the PCA allowed to summarize the API batch-to-batch variability in only three PCs which can be used in future drug product development studies to quantitatively evaluate the impact of the API raw material variability upon the drug product process. The approach described in this article could be applied to any other compound which is prone to batch-to-batch variability. Copyright © 2018 Elsevier B.V. All rights reserved.

  3. Lifelong learning of human actions with deep neural network self-organization.

    PubMed

    Parisi, German I; Tani, Jun; Weber, Cornelius; Wermter, Stefan

    2017-12-01

    Lifelong learning is fundamental in autonomous robotics for the acquisition and fine-tuning of knowledge through experience. However, conventional deep neural models for action recognition from videos do not account for lifelong learning but rather learn a batch of training data with a predefined number of action classes and samples. Thus, there is the need to develop learning systems with the ability to incrementally process available perceptual cues and to adapt their responses over time. We propose a self-organizing neural architecture for incrementally learning to classify human actions from video sequences. The architecture comprises growing self-organizing networks equipped with recurrent neurons for processing time-varying patterns. We use a set of hierarchically arranged recurrent networks for the unsupervised learning of action representations with increasingly large spatiotemporal receptive fields. Lifelong learning is achieved in terms of prediction-driven neural dynamics in which the growth and the adaptation of the recurrent networks are driven by their capability to reconstruct temporally ordered input sequences. Experimental results on a classification task using two action benchmark datasets show that our model is competitive with state-of-the-art methods for batch learning also when a significant number of sample labels are missing or corrupted during training sessions. Additional experiments show the ability of our model to adapt to non-stationary input avoiding catastrophic interference. Copyright © 2017 The Author(s). Published by Elsevier Ltd.. All rights reserved.

  4. 33 CFR 150.514 - What are the annual tests and inspections?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... boat, and liferaft. At that time, the operator must also empty, clean, and refill each fuel tank with fresh fuel; (b) Thoroughly inspect and, if needed, repair each davit, winch, fall, and other launching... that has passed; (d) Check all lifesaving equipment batteries and replace any battery that is marked...

  5. 46 CFR 199.150 - Survival craft launching and recovery arrangements; general.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... appliance for a lifeboat must be approved under approval series 160.132 with a winch approved under approval series 160.115. (2) Each launching appliance for a davit-launched liferaft must be approved under approval series 160.163 with an automatic disengaging apparatus approved under approval series 160.170. (b...

  6. 46 CFR 199.150 - Survival craft launching and recovery arrangements; general.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... appliance for a lifeboat must be approved under approval series 160.132 with a winch approved under approval series 160.115. (2) Each launching appliance for a davit-launched liferaft must be approved under approval series 160.163 with an automatic disengaging apparatus approved under approval series 160.170. (b...

  7. 46 CFR 160.054-4 - Contents.

    Code of Federal Regulations, 2010 CFR

    1997-10-01

    ... 46 Shipping 6 1997-10-01 1997-10-01 false Contents. 160.054-4 Section 160.054-4 EQUIPMENT... Liferafts § 160.054-4 Contents. (a) Individual cartons. Cartons and their contents shall be the type...) Instructions. Instructions for the use of the contents of the first-aid kit shall be printed in legible type...

  8. 33 CFR 150.514 - What are the annual tests and inspections?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ..., the operator must: (a) Strip, clean, thoroughly inspect, and, if needed, repair each lifeboat, rescue boat, and liferaft. At that time, the operator must also empty, clean, and refill each fuel tank with fresh fuel; (b) Thoroughly inspect and, if needed, repair each davit, winch, fall, and other launching...

  9. 33 CFR 150.514 - What are the annual tests and inspections?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ..., the operator must: (a) Strip, clean, thoroughly inspect, and, if needed, repair each lifeboat, rescue boat, and liferaft. At that time, the operator must also empty, clean, and refill each fuel tank with fresh fuel; (b) Thoroughly inspect and, if needed, repair each davit, winch, fall, and other launching...

  10. 33 CFR 150.514 - What are the annual tests and inspections?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ..., the operator must: (a) Strip, clean, thoroughly inspect, and, if needed, repair each lifeboat, rescue boat, and liferaft. At that time, the operator must also empty, clean, and refill each fuel tank with fresh fuel; (b) Thoroughly inspect and, if needed, repair each davit, winch, fall, and other launching...

  11. 33 CFR 150.514 - What are the annual tests and inspections?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ..., the operator must: (a) Strip, clean, thoroughly inspect, and, if needed, repair each lifeboat, rescue boat, and liferaft. At that time, the operator must also empty, clean, and refill each fuel tank with fresh fuel; (b) Thoroughly inspect and, if needed, repair each davit, winch, fall, and other launching...

  12. 46 CFR 117.202 - Survival craft-vessels operating on oceans routes.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 4 2013-10-01 2013-10-01 false Survival craft-vessels operating on oceans routes. 117... operating on oceans routes. (a) Except as allowed by paragraph (b) of this section, each vessel certificated to operate on an oceans route in cold water must be provided with inflatable liferafts of an...

  13. 46 CFR 117.202 - Survival craft-vessels operating on oceans routes.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 4 2014-10-01 2014-10-01 false Survival craft-vessels operating on oceans routes. 117... operating on oceans routes. (a) Except as allowed by paragraph (b) of this section, each vessel certificated to operate on an oceans route in cold water must be provided with inflatable liferafts of an...

  14. 46 CFR 117.202 - Survival craft-vessels operating on oceans routes.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 46 Shipping 4 2012-10-01 2012-10-01 false Survival craft-vessels operating on oceans routes. 117... operating on oceans routes. (a) Except as allowed by paragraph (b) of this section, each vessel certificated to operate on an oceans route in cold water must be provided with inflatable liferafts of an...

  15. 46 CFR 117.202 - Survival craft-vessels operating on oceans routes.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 46 Shipping 4 2011-10-01 2011-10-01 false Survival craft-vessels operating on oceans routes. 117... operating on oceans routes. (a) Except as allowed by paragraph (b) of this section, each vessel certificated to operate on an oceans route in cold water must be provided with inflatable liferafts of an...

  16. 46 CFR 117.202 - Survival craft-vessels operating on oceans routes.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Survival craft-vessels operating on oceans routes. 117... operating on oceans routes. (a) Except as allowed by paragraph (b) of this section, each vessel certificated to operate on an oceans route in cold water must be provided with inflatable liferafts of an...

  17. Frogmen on Apollo command module boilerplate flotation collar during recovery

    NASA Image and Video Library

    1966-02-22

    Apollo command module boilerplate floats in the Atlantic Ocean during a practice recovery exercise. Frogmen in a liferaft and on the flotation collar secure the command module boilerplate for hoisting onto a nearby recovery ship. The exercise was conducted in preparation for the forthcoming Apollo-Saturn 201 (AS-201) mission.

  18. 46 CFR 160.023-6 - Container.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Container. 160.023-6 Section 160.023-6 Shipping COAST... Container. (a) General. The container for storing the signals on lifeboats and liferafts is not required to be of a special design or be approved by the Coast Guard. The container must meet the requirements in...

  19. 46 CFR 160.037-6 - Container.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Container. 160.037-6 Section 160.037-6 Shipping COAST...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Hand Orange Smoke Distress Signals § 160.037-6 Container. (a) General. The container for storing the signals on lifeboats and liferafts is not required to be of a...

  20. 46 CFR 160.170-23 - Procedure for approval of design, material, or construction change.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 46 Shipping 6 2014-10-01 2014-10-01 false Procedure for approval of design, material, or construction change. 160.170-23 Section 160.170-23 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Davit-Launched Liferaft Automatic Release Hooks ...

  1. 46 CFR 160.170-23 - Procedure for approval of design, material, or construction change.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 46 Shipping 6 2013-10-01 2013-10-01 false Procedure for approval of design, material, or construction change. 160.170-23 Section 160.170-23 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Davit-Launched Liferaft Automatic Release Hooks ...

  2. 46 CFR 160.054-2 - Type and size.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-2 Type and size. (a) Type. First-aid kits covered by this specification shall be of the water-tight type... special consideration. (b) Size. First-aid kits shall be of a size adequate for packing 12 standard single...

  3. 46 CFR 160.054-2 - Type and size.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-2 Type and size. (a) Type. First-aid kits covered by this specification shall be of the water-tight type... special consideration. (b) Size. First-aid kits shall be of a size adequate for packing 12 standard single...

  4. 46 CFR 160.054-7 - Procedure for approval.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-7 Procedure... the first-aid kit. At the time of selection of the pre-approval sample, the manufacturer shall furnish... § 160.054-5 to determine the suitability of the first-aid kit for use in conjunction with lifesaving...

  5. 46 CFR 160.054-2 - Type and size.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-2 Type and size. (a) Type. First-aid kits covered by this specification shall be of the water-tight type... special consideration. (b) Size. First-aid kits shall be of a size adequate for packing 12 standard single...

  6. 46 CFR 160.054-7 - Procedure for approval.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-7 Procedure... the first-aid kit. At the time of selection of the pre-approval sample, the manufacturer shall furnish... § 160.054-5 to determine the suitability of the first-aid kit for use in conjunction with lifesaving...

  7. 46 CFR 160.054-7 - Procedure for approval.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-7 Procedure... the first-aid kit. At the time of selection of the pre-approval sample, the manufacturer shall furnish... § 160.054-5 to determine the suitability of the first-aid kit for use in conjunction with lifesaving...

  8. 46 CFR 160.054-7 - Procedure for approval.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-7 Procedure... the first-aid kit. At the time of selection of the pre-approval sample, the manufacturer shall furnish... § 160.054-5 to determine the suitability of the first-aid kit for use in conjunction with lifesaving...

  9. 46 CFR 160.054-2 - Type and size.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-2 Type and size. (a) Type. First-aid kits covered by this specification shall be of the water-tight type... special consideration. (b) Size. First-aid kits shall be of a size adequate for packing 12 standard single...

  10. 46 CFR 160.151-29 - Additional approval tests for SOLAS A and SOLAS B inflatable liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT... appendage must be attached to a testing jig similar in material and construction to the appendage's intended... water. The dry weight must be recorded. (2) The appendage and jig must then be quickly lowered into the...

  11. 46 CFR 160.151-57 - Servicing procedure.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... weeks and be checked for leakage by weighing before being installed in a liferaft. An alternative... hydrostatic test under 49 CFR 180.205 must be tested and marked in accordance with that section. (f) At every... the servicing facility to maintain clearance during the test, a person weighing not less than 75 kg...

  12. 46 CFR 160.151-57 - Servicing procedure.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... weeks and be checked for leakage by weighing before being installed in a liferaft. An alternative... hydrostatic test under 49 CFR 180.205 must be tested and marked in accordance with that section. (f) At every... the servicing facility to maintain clearance during the test, a person weighing not less than 75 kg...

  13. 46 CFR 160.151-57 - Servicing procedure.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... weeks and be checked for leakage by weighing before being installed in a liferaft. An alternative... hydrostatic test under 49 CFR 180.205 must be tested and marked in accordance with that section. (f) At every... the servicing facility to maintain clearance during the test, a person weighing not less than 75 kg...

  14. 46 CFR 160.151-45 - Equipment required for servicing facilities.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... and bulletins in effect as indicated on the annual list issued in accordance with § 160.151-35(b)(2...) A source of clean, dry, pressurized air; hoses; and attachments for inflating liferafts; (h) A..., except for items of equipment that are readily available; (p) A means for load-testing davit-launched...

  15. 46 CFR 160.054-2 - Type and size.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-2 Type and size. (a) Type. First-aid kits covered by this specification shall be of the water-tight type... special consideration. (b) Size. First-aid kits shall be of a size adequate for packing 12 standard single...

  16. 46 CFR 160.151-59 - Operating instructions and information for the ship's training manual.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Operating instructions and information for the ship's training manual. 160.151-59 Section 160.151-59 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Inflatable Liferafts (SOLAS) § 160.151-59...

  17. 46 CFR § 160.054-4 - Contents.

    Code of Federal Regulations, 2010 CFR

    2006-10-01

    ... 46 Shipping 6 2006-10-01 2006-10-01 false Contents. § 160.054-4 Section § 160.054-4 Shipping Coast Guard, Department of Homeland Security (Continued) EQUIPMENT, CONSTRUCTION, AND MATERIALS: SPECIFICATIONS AND APPROVAL LIFESAVING EQUIPMENT Kits, First-Aid, for Inflatable Liferafts § 160.054-4 Contents. (a) Individual cartons. Cartons and their...

  18. 46 CFR 160.151-57 - Servicing procedure.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...) It has no expiration date; or (iii) It is to return to service in an item of survival equipment, but... its weight loss exceeds five percent of the weight of the charge, it must be recharged. (c) When an... servicing of a davit-launched liferaft, the launching-load test in paragraph 2/5.2 of IMO Resolution A.689...

  19. 46 CFR 160.036-6 - Container.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 46 Shipping 6 2010-10-01 2010-10-01 false Container. 160.036-6 Section 160.036-6 Shipping COAST... § 160.036-6 Container. (a) General. The container for storing the signals on lifeboats and liferafts is not required to be of a special design or be approved by the Coast Guard. The container must meet the...

  20. 46 CFR 133.105 - Survival craft.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... approval series 160.151 and be equipped with a SOLAS A pack; (ii) On an OSV on an oceans route limited to within 50 nautical miles of the shore, must be approved under approval series 160.151 and be equipped... under approval series 160.051 or 160.151, with any approved equipment pack. (2) Each rigid liferaft must...

  1. Comparison of the release of constituents from granular materials under batch and column testing.

    PubMed

    Lopez Meza, Sarynna; Garrabrants, Andrew C; van der Sloot, Hans; Kosson, David S

    2008-01-01

    Column leaching testing can be considered a better basis for assessing field impact data than any other available batch test method and thus provides a fundamental basis from which to estimate constituent release under a variety of field conditions. However, column testing is time-intensive compared to the more simplified batch testing, and may not always be a viable option when making decisions for material reuse. Batch tests are used most frequently as a simple tool for compliance or quality control reasons. Therefore, it is important to compare the release that occurs under batch and column testing, and establish conservative interpretation protocols for extrapolation from batch data when column data are not available. Five different materials (concrete, construction debris, aluminum recycling residue, coal fly ash and bottom ash) were evaluated via batch and column testing, including different column flow regimes (continuously saturated and intermittent unsaturated flow). Constituent release data from batch and column tests were compared. Results showed no significant difference between the column flow regimes when constituent release data from batch and column tests were compared. In most cases batch and column testing agreed when presented in the form of cumulative release. For arsenic in carbonated materials, however, batch testing underestimates the column constituent release for most LS ratios and also on a cumulative basis. For cases when As is a constituent of concern, column testing may be required.

  2. Batch effects in single-cell RNA-sequencing data are corrected by matching mutual nearest neighbors.

    PubMed

    Haghverdi, Laleh; Lun, Aaron T L; Morgan, Michael D; Marioni, John C

    2018-06-01

    Large-scale single-cell RNA sequencing (scRNA-seq) data sets that are produced in different laboratories and at different times contain batch effects that may compromise the integration and interpretation of the data. Existing scRNA-seq analysis methods incorrectly assume that the composition of cell populations is either known or identical across batches. We present a strategy for batch correction based on the detection of mutual nearest neighbors (MNNs) in the high-dimensional expression space. Our approach does not rely on predefined or equal population compositions across batches; instead, it requires only that a subset of the population be shared between batches. We demonstrate the superiority of our approach compared with existing methods by using both simulated and real scRNA-seq data sets. Using multiple droplet-based scRNA-seq data sets, we demonstrate that our MNN batch-effect-correction method can be scaled to large numbers of cells.

  3. Methods that remove batch effects while retaining group differences may lead to exaggerated confidence in downstream analyses

    PubMed Central

    Nygaard, Vegard; Rødland, Einar Andreas; Hovig, Eivind

    2016-01-01

    Removal of, or adjustment for, batch effects or center differences is generally required when such effects are present in data. In particular, when preparing microarray gene expression data from multiple cohorts, array platforms, or batches for later analyses, batch effects can have confounding effects, inducing spurious differences between study groups. Many methods and tools exist for removing batch effects from data. However, when study groups are not evenly distributed across batches, actual group differences may induce apparent batch differences, in which case batch adjustments may bias, usually deflate, group differences. Some tools therefore have the option of preserving the difference between study groups, e.g. using a two-way ANOVA model to simultaneously estimate both group and batch effects. Unfortunately, this approach may systematically induce incorrect group differences in downstream analyses when groups are distributed between the batches in an unbalanced manner. The scientific community seems to be largely unaware of how this approach may lead to false discoveries. PMID:26272994

  4. Batch statistical process control of a fluid bed granulation process using in-line spatial filter velocimetry and product temperature measurements.

    PubMed

    Burggraeve, A; Van den Kerkhof, T; Hellings, M; Remon, J P; Vervaet, C; De Beer, T

    2011-04-18

    Fluid bed granulation is a batch process, which is characterized by the processing of raw materials for a predefined period of time, consisting of a fixed spraying phase and a subsequent drying period. The present study shows the multivariate statistical modeling and control of a fluid bed granulation process based on in-line particle size distribution (PSD) measurements (using spatial filter velocimetry) combined with continuous product temperature registration using a partial least squares (PLS) approach. Via the continuous in-line monitoring of the PSD and product temperature during granulation of various reference batches, a statistical batch model was developed allowing the real-time evaluation and acceptance or rejection of future batches. Continuously monitored PSD and product temperature process data of 10 reference batches (X-data) were used to develop a reference batch PLS model, regressing the X-data versus the batch process time (Y-data). Two PLS components captured 98.8% of the variation in the X-data block. Score control charts in which the average batch trajectory and upper and lower control limits are displayed were developed. Next, these control charts were used to monitor 4 new test batches in real-time and to immediately detect any deviations from the expected batch trajectory. By real-time evaluation of new batches using the developed control charts and by computation of contribution plots of deviating process behavior at a certain time point, batch losses or reprocessing can be prevented. Immediately after batch completion, all PSD and product temperature information (i.e., a batch progress fingerprint) was used to estimate some granule properties (density and flowability) at an early stage, which can improve batch release time. Individual PLS models relating the computed scores (X) of the reference PLS model (based on the 10 reference batches) and the density, respectively, flowabililty as Y-matrix, were developed. The scores of the 4 test batches were used to examine the predictive ability of the model. Copyright © 2011 Elsevier B.V. All rights reserved.

  5. 46 CFR 160.051-11 - Equipment required for Coastal Service inflatable liferafts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...-21(i) of this part. (h) Flashlight. One flashlight with spare batteries as described in § 160.151-21(m). (i) Signalling mirror. One signalling mirror as described in § 160.151-21(o). (j) Survival... sealing clamps or plugs as described in § 160.151-21(y)(1). (m) Pump or bellows. One pump or bellows as...

  6. Plant-Scale Concentration Column Designs for SHINE Target Solution Utilizing AG 1 Anion Exchange Resin

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Stepinski, Dominique C.; Vandegrift, G. F.

    2015-09-30

    Argonne is assisting SHINE Medical Technologies (SHINE) in their efforts to develop SHINE, an accelerator-driven process that will utilize a uranyl-sulfate solution for the production of fission product Mo-99. An integral part of the process is the development of a column for the separation and recovery of Mo-99, followed by a concentration column to reduce the product volume from 15-25 L to <1 L. Argonne has collected data from batch studies and breakthrough column experiments to utilize the VERSE (Versatile Reaction Separation) simulation program (Purdue University) to design plant-scale product recovery and concentration processes.

  7. SEWAGE OFF-GAS-DRIVEN FUEL CELLS TO STIMULATE RURAL ELECTRIFICATION

    EPA Science Inventory

    Literature reviews confirmed the feasibility of the system relying on methane to supply the fuel cell and the waste heat from the subsequent fuel cell operation driving the decomposition process. A batch bioreactor and a proton exchange fuel cell at the lab scale are used to c...

  8. Removing Batch Effects from Longitudinal Gene Expression - Quantile Normalization Plus ComBat as Best Approach for Microarray Transcriptome Data

    PubMed Central

    Müller, Christian; Schillert, Arne; Röthemeier, Caroline; Trégouët, David-Alexandre; Proust, Carole; Binder, Harald; Pfeiffer, Norbert; Beutel, Manfred; Lackner, Karl J.; Schnabel, Renate B.; Tiret, Laurence; Wild, Philipp S.; Blankenberg, Stefan

    2016-01-01

    Technical variation plays an important role in microarray-based gene expression studies, and batch effects explain a large proportion of this noise. It is therefore mandatory to eliminate technical variation while maintaining biological variability. Several strategies have been proposed for the removal of batch effects, although they have not been evaluated in large-scale longitudinal gene expression data. In this study, we aimed at identifying a suitable method for batch effect removal in a large study of microarray-based longitudinal gene expression. Monocytic gene expression was measured in 1092 participants of the Gutenberg Health Study at baseline and 5-year follow up. Replicates of selected samples were measured at both time points to identify technical variability. Deming regression, Passing-Bablok regression, linear mixed models, non-linear models as well as ReplicateRUV and ComBat were applied to eliminate batch effects between replicates. In a second step, quantile normalization prior to batch effect correction was performed for each method. Technical variation between batches was evaluated by principal component analysis. Associations between body mass index and transcriptomes were calculated before and after batch removal. Results from association analyses were compared to evaluate maintenance of biological variability. Quantile normalization, separately performed in each batch, combined with ComBat successfully reduced batch effects and maintained biological variability. ReplicateRUV performed perfectly in the replicate data subset of the study, but failed when applied to all samples. All other methods did not substantially reduce batch effects in the replicate data subset. Quantile normalization plus ComBat appears to be a valuable approach for batch correction in longitudinal gene expression data. PMID:27272489

  9. A novel data-driven learning method for radar target detection in nonstationary environments

    DOE PAGES

    Akcakaya, Murat; Nehorai, Arye; Sen, Satyabrata

    2016-04-12

    Most existing radar algorithms are developed under the assumption that the environment (clutter) is stationary. However, in practice, the characteristics of the clutter can vary enormously depending on the radar-operational scenarios. If unaccounted for, these nonstationary variabilities may drastically hinder the radar performance. Therefore, to overcome such shortcomings, we develop a data-driven method for target detection in nonstationary environments. In this method, the radar dynamically detects changes in the environment and adapts to these changes by learning the new statistical characteristics of the environment and by intelligibly updating its statistical detection algorithm. Specifically, we employ drift detection algorithms to detectmore » changes in the environment; incremental learning, particularly learning under concept drift algorithms, to learn the new statistical characteristics of the environment from the new radar data that become available in batches over a period of time. The newly learned environment characteristics are then integrated in the detection algorithm. Furthermore, we use Monte Carlo simulations to demonstrate that the developed method provides a significant improvement in the detection performance compared with detection techniques that are not aware of the environmental changes.« less

  10. Simultaneous Transformation of Commingled Trichloroethylene, Tetrachloroethylene, and 1,4-Dioxane by a Microbially Driven Fenton Reaction in Batch Liquid Cultures.

    PubMed

    Sekar, Ramanan; Taillefert, Martial; DiChristina, Thomas J

    2016-11-01

    Improper disposal of 1,4-dioxane and the chlorinated organic solvents trichloroethylene (TCE) and tetrachloroethylene (also known as perchloroethylene [PCE]) has resulted in widespread contamination of soil and groundwater. In the present study, a previously designed microbially driven Fenton reaction system was reconfigured to generate hydroxyl (HO˙) radicals for simultaneous transformation of source zone levels of single, binary, and ternary mixtures of TCE, PCE, and 1,4-dioxane. The reconfigured Fenton reaction system was driven by fed batch cultures of the Fe(III)-reducing facultative anaerobe Shewanella oneidensis amended with lactate, Fe(III), and contaminants and exposed to alternating anaerobic and aerobic conditions. To avoid contaminant loss due to volatility, the Fe(II)-generating, hydrogen peroxide-generating, and contaminant transformation phases of the microbially driven Fenton reaction system were separated. The reconfigured Fenton reaction system transformed TCE, PCE, and 1,4-dioxane either as single contaminants or as binary and ternary mixtures. In the presence of equimolar concentrations of PCE and TCE, the ratio of the experimentally derived rates of PCE and TCE transformation was nearly identical to the ratio of the corresponding HO˙ radical reaction rate constants. The reconfigured Fenton reaction system may be applied as an ex situ platform for simultaneous degradation of commingled TCE, PCE, and 1,4-dioxane and provides valuable information for future development of in situ remediation technologies. A microbially driven Fenton reaction system [driven by the Fe(III)-reducing facultative anaerobe S. oneidensis] was reconfigured to transform source zone levels of TCE, PCE, and 1,4-dioxane as single contaminants or as binary and ternary mixtures. The microbially driven Fenton reaction may thus be applied as an ex situ platform for simultaneous degradation of at least three (and potentially more) commingled contaminants. Additional targets for ex situ and in situ degradation by the microbially driven Fenton reaction developed in the present study include multiple combinations of environmental contaminants susceptible to attack by Fenton reaction-generated HO˙ radicals, including commingled plumes of 1,4-dioxane, pentachlorophenol (PCP), PCE, TCE, 1,1,2-trichloroethane (TCA), and perfluoroalkylated substances (PFAS). Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  11. Simultaneous Transformation of Commingled Trichloroethylene, Tetrachloroethylene, and 1,4-Dioxane by a Microbially Driven Fenton Reaction in Batch Liquid Cultures

    PubMed Central

    Sekar, Ramanan; Taillefert, Martial

    2016-01-01

    ABSTRACT Improper disposal of 1,4-dioxane and the chlorinated organic solvents trichloroethylene (TCE) and tetrachloroethylene (also known as perchloroethylene [PCE]) has resulted in widespread contamination of soil and groundwater. In the present study, a previously designed microbially driven Fenton reaction system was reconfigured to generate hydroxyl (HO˙) radicals for simultaneous transformation of source zone levels of single, binary, and ternary mixtures of TCE, PCE, and 1,4-dioxane. The reconfigured Fenton reaction system was driven by fed batch cultures of the Fe(III)-reducing facultative anaerobe Shewanella oneidensis amended with lactate, Fe(III), and contaminants and exposed to alternating anaerobic and aerobic conditions. To avoid contaminant loss due to volatility, the Fe(II)-generating, hydrogen peroxide-generating, and contaminant transformation phases of the microbially driven Fenton reaction system were separated. The reconfigured Fenton reaction system transformed TCE, PCE, and 1,4-dioxane either as single contaminants or as binary and ternary mixtures. In the presence of equimolar concentrations of PCE and TCE, the ratio of the experimentally derived rates of PCE and TCE transformation was nearly identical to the ratio of the corresponding HO˙ radical reaction rate constants. The reconfigured Fenton reaction system may be applied as an ex situ platform for simultaneous degradation of commingled TCE, PCE, and 1,4-dioxane and provides valuable information for future development of in situ remediation technologies. IMPORTANCE A microbially driven Fenton reaction system [driven by the Fe(III)-reducing facultative anaerobe S. oneidensis] was reconfigured to transform source zone levels of TCE, PCE, and 1,4-dioxane as single contaminants or as binary and ternary mixtures. The microbially driven Fenton reaction may thus be applied as an ex situ platform for simultaneous degradation of at least three (and potentially more) commingled contaminants. Additional targets for ex situ and in situ degradation by the microbially driven Fenton reaction developed in the present study include multiple combinations of environmental contaminants susceptible to attack by Fenton reaction-generated HO˙ radicals, including commingled plumes of 1,4-dioxane, pentachlorophenol (PCP), PCE, TCE, 1,1,2-trichloroethane (TCA), and perfluoroalkylated substances (PFAS). PMID:27542932

  12. Maritime Mass Rescue Interventions: Availability and Associated Technology

    DTIC Science & Technology

    2010-12-01

    MOR Liferaft Description: The Zodiac MOR (Means of Rescue) was specially developed to meet the latest requirements introduced for Roll On/Roll Off...Mass Rescue Interventions; Availability and Associated Technology NOTICE This document is disseminated under the sponsorship of the Department of...Homeland Security in the interest of information exchange. The United States Government assumes no liability for its contents or use thereof. The

  13. 46 CFR 120.434 - Lifeboat and liferaft floodlights on vessels of more than 19.8 meters (65 feet) in length with...

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... than 19.8 meters (65 feet) in length with overnight accommodations for more than 49 passengers. 120.434... (65 feet) in length with overnight accommodations for more than 49 passengers. Each vessel of more than 19.8 meters (65 feet) in length carrying more than 600 passengers or with overnight accommodations...

  14. 46 CFR 120.434 - Lifeboat and liferaft floodlights on vessels of more than 19.8 meters (65 feet) in length with...

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... than 19.8 meters (65 feet) in length with overnight accommodations for more than 49 passengers. 120.434... (65 feet) in length with overnight accommodations for more than 49 passengers. Each vessel of more than 19.8 meters (65 feet) in length carrying more than 600 passengers or with overnight accommodations...

  15. 46 CFR 160.151-21 - Equipment required for SOLAS A and SOLAS B inflatable liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ...). (g) Tin-opener (Regulation -III/38.5.1.7). Each sharp part of a tin-opener described by Regulation... fishing tackle must be in a kit approved by the Commandant under approval series 160.061. (r) Food rations.... (s) Drinking water (Regulation -III/38.5.1.19). The fresh water required by Regulation III/38.5.1.19...

  16. 46 CFR 160.151-21 - Equipment required for SOLAS A and SOLAS B inflatable liferafts.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ...). (g) Tin-opener (Regulation -III/38.5.1.7). Each sharp part of a tin-opener described by Regulation... fishing tackle must be in a kit approved by the Commandant under approval series 160.061. (r) Food rations.... (s) Drinking water (Regulation -III/38.5.1.19). The fresh water required by Regulation III/38.5.1.19...

  17. 46 CFR 160.051-9 - Equipment required for Coastal Service inflatable liferafts.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... line as described in § 160.151-21(a). (b) Knife. One knife, of a type designed to minimize the chance... anchor as described in § 160.151-21(e). (f) Paddles. Two paddles of the same size and type as used to... as described in § 160.151-21(i) of this part. (h) Flashlight. One flashlight with spare batteries as...

  18. 46 CFR 160.051-11 - Equipment required for Coastal Service inflatable liferafts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... line as described in § 160.151-21(a). (b) Knife. One knife, of a type designed to minimize the chance... anchor as described in § 160.151-21(e). (f) Paddles. Two paddles of the same size and type as used to...-21(i) of this part. (h) Flashlight. One flashlight with spare batteries as described in § 160.151-21...

  19. 46 CFR 160.051-11 - Equipment required for Coastal Service inflatable liferafts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... line as described in § 160.151-21(a). (b) Knife. One knife, of a type designed to minimize the chance... anchor as described in § 160.151-21(e). (f) Paddles. Two paddles of the same size and type as used to...-21(i) of this part. (h) Flashlight. One flashlight with spare batteries as described in § 160.151-21...

  20. 33 CFR 149.303 - What survival craft and rescue boats may be used on a manned deepwater port?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... boats may be used on a manned deepwater port? 149.303 Section 149.303 Navigation and Navigable Waters... craft and rescue boats may be used on a manned deepwater port? (a) Each survival craft on a manned... subpart; or (2) A liferaft meeting the requirements of § 149.308 of this subpart. (b) Each rescue boat on...

  1. 33 CFR 149.303 - What survival craft and rescue boats may be used on a manned deepwater port?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... boats may be used on a manned deepwater port? 149.303 Section 149.303 Navigation and Navigable Waters... craft and rescue boats may be used on a manned deepwater port? (a) Each survival craft on a manned... subpart; or (2) A liferaft meeting the requirements of § 149.308 of this subpart. (b) Each rescue boat on...

  2. 33 CFR 149.303 - What survival craft and rescue boats may be used on a manned deepwater port?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... boats may be used on a manned deepwater port? 149.303 Section 149.303 Navigation and Navigable Waters... craft and rescue boats may be used on a manned deepwater port? (a) Each survival craft on a manned... subpart; or (2) A liferaft meeting the requirements of § 149.308 of this subpart. (b) Each rescue boat on...

  3. Fault detection and diagnosis in an industrial fed-batch cell culture process.

    PubMed

    Gunther, Jon C; Conner, Jeremy S; Seborg, Dale E

    2007-01-01

    A flexible process monitoring method was applied to industrial pilot plant cell culture data for the purpose of fault detection and diagnosis. Data from 23 batches, 20 normal operating conditions (NOC) and three abnormal, were available. A principal component analysis (PCA) model was constructed from 19 NOC batches, and the remaining NOC batch was used for model validation. Subsequently, the model was used to successfully detect (both offline and online) abnormal process conditions and to diagnose the root causes. This research demonstrates that data from a relatively small number of batches (approximately 20) can still be used to monitor for a wide range of process faults.

  4. Batch calculations in CalcHEP

    NASA Astrophysics Data System (ADS)

    Pukhov, A.

    2003-04-01

    CalcHEP is a clone of the CompHEP project which is developed by the author outside of the CompHEP group. CompHEP/CalcHEP are packages for automatic calculations of elementary particle decay and collision properties in the lowest order of perturbation theory. The main idea prescribed into the packages is to make available passing on from the Lagrangian to the final distributions effectively with a high level of automation. According to this, the packages were created as a menu driven user friendly programs for calculations in the interactive mode. From the other side, long-time calculations should be done in the non-interactive regime. Thus, from the beginning CompHEP has a problem of batch calculations. In CompHEP 33.23 the batch session was realized by mean of interactive menu which allows to the user to formulate the task for batch. After that the not-interactive session was launched. This way is too restricted, not flexible, and leads to doubling in programming. In this article I discuss another approach how one can force an interactive program to work in non-interactive mode. This approach was realized in CalcHEP 2.1 disposed on http://theory.sinp.msu.ru/~pukhov/calchep.html.

  5. A new statistic for identifying batch effects in high-throughput genomic data that uses guided principal component analysis.

    PubMed

    Reese, Sarah E; Archer, Kellie J; Therneau, Terry M; Atkinson, Elizabeth J; Vachon, Celine M; de Andrade, Mariza; Kocher, Jean-Pierre A; Eckel-Passow, Jeanette E

    2013-11-15

    Batch effects are due to probe-specific systematic variation between groups of samples (batches) resulting from experimental features that are not of biological interest. Principal component analysis (PCA) is commonly used as a visual tool to determine whether batch effects exist after applying a global normalization method. However, PCA yields linear combinations of the variables that contribute maximum variance and thus will not necessarily detect batch effects if they are not the largest source of variability in the data. We present an extension of PCA to quantify the existence of batch effects, called guided PCA (gPCA). We describe a test statistic that uses gPCA to test whether a batch effect exists. We apply our proposed test statistic derived using gPCA to simulated data and to two copy number variation case studies: the first study consisted of 614 samples from a breast cancer family study using Illumina Human 660 bead-chip arrays, whereas the second case study consisted of 703 samples from a family blood pressure study that used Affymetrix SNP Array 6.0. We demonstrate that our statistic has good statistical properties and is able to identify significant batch effects in two copy number variation case studies. We developed a new statistic that uses gPCA to identify whether batch effects exist in high-throughput genomic data. Although our examples pertain to copy number data, gPCA is general and can be used on other data types as well. The gPCA R package (Available via CRAN) provides functionality and data to perform the methods in this article. reesese@vcu.edu

  6. Continuous flow operation with appropriately adjusting composites in influent for recovery of Cr(VI), Cu(II) and Cd(II) in self-driven MFC-MEC system.

    PubMed

    Li, Ming; Pan, Yuzhen; Huang, Liping; Zhang, Yong; Yang, Jinhui

    2017-03-01

    A self-driven microbial fuel cell (MFC) - microbial electrolysis cell (MEC) system, where electricity generated from MFCs is in situ utilized for powering MECs, has been previously reported for recovering Cr(VI), Cu(II) and Cd(II) with individual metals fed in different units of the system in batch operation. Here it was advanced with treating synthetic mixed metals' solution at appropriately adjusting composites in fed-batch and continuous flow operations for complete separation of Cr(VI), Cu(II) and Cd(II) from each other. Under an optimal condition of hydraulic residence time of 4 h, matching of two serially connected MFCs with one MEC, and fed with a composite of either 5 mg L -1 Cr(VI), 1 mg L -1 Cu(II) and 5 mg L -1 Cd(II), or 1 mg L -1 Cr(VI), 5 mg L -1 Cu(II) and 5 mg L -1 Cd(II), the self-driven MFC-MEC system can completely and sequentially recover Cu(II), Cr(VI) and Cd(II) from mixed metals. This study provides a true sustainable and zero-energy-consumed approach of using bioelectrochemical systems for completely recovering and separating Cr(VI), Cu(II) and Cd(II) from each other or from wastes or contaminated sites.

  7. Survival in Cold Waters: Staying Alive

    DTIC Science & Technology

    2003-01-01

    19 CHAPTER 2: HOW CAN WE PROTECT FROM THE FOUR PHYSIOLOGICAL STAGES OF COLD WATER IMMERSION...carry liferafts that can easily be launched and boarded by the entire crew and passengers. (c) The only exception to this should be where it is...water into the system will reduce insulation by 30% 2 (d) the maximum insulation that can be added to a suit to prevent heat loss and still be wearable

  8. 46 CFR 160.151-21 - Equipment required for SOLAS A and SOLAS B inflatable liferafts.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ...), Chapter IV/4.1.5.1.7 and ISO 18813). Each sharp part of a tin-opener must have a guard. (h) First-aid kit... approval series 160.061. (r) Food rations (IMO LSA Code, as amended by Resolution MSC.293(87), Chapter IV/4.1.5.1.18) The food rations must be approved by the Commandant under approval series 160.046. (s...

  9. 46 CFR 160.151-21 - Equipment required for SOLAS A and SOLAS B inflatable liferafts.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ...), Chapter IV/4.1.5.1.7 and ISO 18813). Each sharp part of a tin-opener must have a guard. (h) First-aid kit... approval series 160.061. (r) Food rations (IMO LSA Code, as amended by Resolution MSC.293(87), Chapter IV/4.1.5.1.18) The food rations must be approved by the Commandant under approval series 160.046. (s...

  10. 46 CFR 160.151-21 - Equipment required for SOLAS A and SOLAS B inflatable liferafts.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ...), Chapter IV/4.1.5.1.7 and ISO 18813). Each sharp part of a tin-opener must have a guard. (h) First-aid kit... approval series 160.061. (r) Food rations (IMO LSA Code, as amended by Resolution MSC.293(87), Chapter IV/4.1.5.1.18) The food rations must be approved by the Commandant under approval series 160.046. (s...

  11. 33 CFR 149.303 - What survival craft and rescue boats may be used on a manned deepwater port?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... boats may be used on a manned deepwater port? 149.303 Section 149.303 Navigation and Navigable Waters... craft and rescue boats may be used on a manned deepwater port? (a) Each survival craft on a manned... part; or (2) A liferaft meeting the requirements of § 149.308 of this part. (b) Each rescue boat on a...

  12. 33 CFR 149.303 - What survival craft and rescue boats may be used on a manned deepwater port?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... boats may be used on a manned deepwater port? 149.303 Section 149.303 Navigation and Navigable Waters... craft and rescue boats may be used on a manned deepwater port? (a) Each survival craft on a manned... part; or (2) A liferaft meeting the requirements of § 149.308 of this part. (b) Each rescue boat on a...

  13. Using Forensics to Untangle Batch Effects in TCGA Data - TCGA

    Cancer.gov

    Rehan Akbani, Ph.D., and colleagues at the University of Texas MD Anderson Cancer Center developed a tool called MBatch to detect, diagnose, and correct batch effects in TCGA data. Read more about batch effects in this Case Study.

  14. Fenton-like Degradation of MTBE: Effects of Iron Counter Anion and Radical Scavengers

    EPA Science Inventory

    Fenton-driven oxidation of Methyl tert-butyl ether (MTBE) (0.11-0.16 mM) in batch reactors containing ferric iron (5 mM), hydrogen peroxide (H2O2) (6 mM) (pH=3) was performed to investigate MTBE transformation mechanisms. Independent variables included the form of iron (Fe) (Fe2(...

  15. U.S.-MEXICO BORDER PROGRAM ARIZONA BORDER STUDY--STANDARD OPERATING PROCEDURE FOR BATCHING OF FIELD DATA FORMS (UA-C-4.0)

    EPA Science Inventory

    The purpose of this SOP is to describe the assembly of household (HH) packets into data processing batches. The batching process enables orderly tracking of packets or forms through data processing and limits the potential for packet or form loss. This procedure was used for th...

  16. NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR BATCHING OF FIELD DATA FORMS (UA-C-4.0)

    EPA Science Inventory

    The purpose of this SOP is to describe the assembly of household (HH) packets into data processing batches. The batching process enables orderly tracking of packets or forms through data processing and limits the potential for packet or form loss. This procedure was used for th...

  17. IceProd 2 Usage Experience

    NASA Astrophysics Data System (ADS)

    Delventhal, D.; Schultz, D.; Diaz Velez, J. C.

    2017-10-01

    IceProd is a data processing and management framework developed by the IceCube Neutrino Observatory for processing of Monte Carlo simulations, detector data, and data driven analysis. It runs as a separate layer on top of grid and batch systems. This is accomplished by a set of daemons which process job workflow, maintaining configuration and status information on the job before, during, and after processing. IceProd can also manage complex workflow DAGs across distributed computing grids in order to optimize usage of resources. IceProd has recently been rewritten to increase its scaling capabilities, handle user analysis workflows together with simulation production, and facilitate the integration with 3rd party scheduling tools. IceProd 2, the second generation of IceProd, has been running in production for several months now. We share our experience setting up the system and things we’ve learned along the way.

  18. Correcting for batch effects in case-control microbiome studies

    PubMed Central

    Gibbons, Sean M.; Duvallet, Claire

    2018-01-01

    High-throughput data generation platforms, like mass-spectrometry, microarrays, and second-generation sequencing are susceptible to batch effects due to run-to-run variation in reagents, equipment, protocols, or personnel. Currently, batch correction methods are not commonly applied to microbiome sequencing datasets. In this paper, we compare different batch-correction methods applied to microbiome case-control studies. We introduce a model-free normalization procedure where features (i.e. bacterial taxa) in case samples are converted to percentiles of the equivalent features in control samples within a study prior to pooling data across studies. We look at how this percentile-normalization method compares to traditional meta-analysis methods for combining independent p-values and to limma and ComBat, widely used batch-correction models developed for RNA microarray data. Overall, we show that percentile-normalization is a simple, non-parametric approach for correcting batch effects and improving sensitivity in case-control meta-analyses. PMID:29684016

  19. Adaptive Batch Mode Active Learning.

    PubMed

    Chakraborty, Shayok; Balasubramanian, Vineeth; Panchanathan, Sethuraman

    2015-08-01

    Active learning techniques have gained popularity to reduce human effort in labeling data instances for inducing a classifier. When faced with large amounts of unlabeled data, such algorithms automatically identify the exemplar and representative instances to be selected for manual annotation. More recently, there have been attempts toward a batch mode form of active learning, where a batch of data points is simultaneously selected from an unlabeled set. Real-world applications require adaptive approaches for batch selection in active learning, depending on the complexity of the data stream in question. However, the existing work in this field has primarily focused on static or heuristic batch size selection. In this paper, we propose two novel optimization-based frameworks for adaptive batch mode active learning (BMAL), where the batch size as well as the selection criteria are combined in a single formulation. We exploit gradient-descent-based optimization strategies as well as properties of submodular functions to derive the adaptive BMAL algorithms. The solution procedures have the same computational complexity as existing state-of-the-art static BMAL techniques. Our empirical results on the widely used VidTIMIT and the mobile biometric (MOBIO) data sets portray the efficacy of the proposed frameworks and also certify the potential of these approaches in being used for real-world biometric recognition applications.

  20. Tier 3 batch system data locality via managed caches

    NASA Astrophysics Data System (ADS)

    Fischer, Max; Giffels, Manuel; Jung, Christopher; Kühn, Eileen; Quast, Günter

    2015-05-01

    Modern data processing increasingly relies on data locality for performance and scalability, whereas the common HEP approaches aim for uniform resource pools with minimal locality, recently even across site boundaries. To combine advantages of both, the High- Performance Data Analysis (HPDA) Tier 3 concept opportunistically establishes data locality via coordinated caches. In accordance with HEP Tier 3 activities, the design incorporates two major assumptions: First, only a fraction of data is accessed regularly and thus the deciding factor for overall throughput. Second, data access may fallback to non-local, making permanent local data availability an inefficient resource usage strategy. Based on this, the HPDA design generically extends available storage hierarchies into the batch system. Using the batch system itself for scheduling file locality, an array of independent caches on the worker nodes is dynamically populated with high-profile data. Cache state information is exposed to the batch system both for managing caches and scheduling jobs. As a result, users directly work with a regular, adequately sized storage system. However, their automated batch processes are presented with local replications of data whenever possible.

  1. Mixture model normalization for non-targeted gas chromatography/mass spectrometry metabolomics data.

    PubMed

    Reisetter, Anna C; Muehlbauer, Michael J; Bain, James R; Nodzenski, Michael; Stevens, Robert D; Ilkayeva, Olga; Metzger, Boyd E; Newgard, Christopher B; Lowe, William L; Scholtens, Denise M

    2017-02-02

    Metabolomics offers a unique integrative perspective for health research, reflecting genetic and environmental contributions to disease-related phenotypes. Identifying robust associations in population-based or large-scale clinical studies demands large numbers of subjects and therefore sample batching for gas-chromatography/mass spectrometry (GC/MS) non-targeted assays. When run over weeks or months, technical noise due to batch and run-order threatens data interpretability. Application of existing normalization methods to metabolomics is challenged by unsatisfied modeling assumptions and, notably, failure to address batch-specific truncation of low abundance compounds. To curtail technical noise and make GC/MS metabolomics data amenable to analyses describing biologically relevant variability, we propose mixture model normalization (mixnorm) that accommodates truncated data and estimates per-metabolite batch and run-order effects using quality control samples. Mixnorm outperforms other approaches across many metrics, including improved correlation of non-targeted and targeted measurements and superior performance when metabolite detectability varies according to batch. For some metrics, particularly when truncation is less frequent for a metabolite, mean centering and median scaling demonstrate comparable performance to mixnorm. When quality control samples are systematically included in batches, mixnorm is uniquely suited to normalizing non-targeted GC/MS metabolomics data due to explicit accommodation of batch effects, run order and varying thresholds of detectability. Especially in large-scale studies, normalization is crucial for drawing accurate conclusions from non-targeted GC/MS metabolomics data.

  2. Future Supply Chains Enabled by Continuous Processing-Opportunities Challenges May 20-21 2014 Continuous Manufacturing Symposium.

    PubMed

    Srai, Jagjit Singh; Badman, Clive; Krumme, Markus; Futran, Mauricio; Johnston, Craig

    2015-03-01

    This paper examines the opportunities and challenges facing the pharmaceutical industry in moving to a primarily "continuous processing"-based supply chain. The current predominantly "large batch" and centralized manufacturing system designed for the "blockbuster" drug has driven a slow-paced, inventory heavy operating model that is increasingly regarded as inflexible and unsustainable. Indeed, new markets and the rapidly evolving technology landscape will drive more product variety, shorter product life-cycles, and smaller drug volumes, which will exacerbate an already unsustainable economic model. Future supply chains will be required to enhance affordability and availability for patients and healthcare providers alike despite the increased product complexity. In this more challenging supply scenario, we examine the potential for a more pull driven, near real-time demand-based supply chain, utilizing continuous processing where appropriate as a key element of a more "flow-through" operating model. In this discussion paper on future supply chain models underpinned by developments in the continuous manufacture of pharmaceuticals, we have set out; The paper recognizes that although current batch operational performance in pharma is far from optimal and not necessarily an appropriate end-state benchmark for batch technology, the adoption of continuous supply chain operating models underpinned by continuous production processing, as full or hybrid solutions in selected product supply chains, can support industry transformations to deliver right-first-time quality at substantially lower inventory profiles. © 2015 The Authors. Journal of Pharmaceutical Sciences published by Wiley Periodicals, Inc. and the American Pharmacists Association. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

  3. An Inversion Analysis of Recent Variability in Natural CO2 Fluxes Using GOSAT and In Situ Observations

    NASA Astrophysics Data System (ADS)

    Wang, J. S.; Kawa, S. R.; Baker, D. F.; Collatz, G. J.; Ott, L. E.

    2015-12-01

    About one-half of the global CO2 emissions from fossil fuel combustion and deforestation accumulates in the atmosphere, where it contributes to global warming. The rest is taken up by vegetation and the ocean. The precise contribution of the two sinks, and their location and year-to-year variability are, however, not well understood. We use two different approaches, batch Bayesian synthesis inversion and variational data assimilation, to deduce the global spatiotemporal distributions of CO2 fluxes during 2009-2010. One of our objectives is to assess different sources of uncertainties in inferred fluxes, including uncertainties in prior flux estimates and observations, and differences in inversion techniques. For prior constraints, we utilize fluxes and uncertainties from the CASA-GFED model of the terrestrial biosphere and biomass burning driven by satellite observations and interannually varying meteorology. We also use measurement-based ocean flux estimates and two sets of fixed fossil CO2 emissions. Here, our inversions incorporate column CO2 measurements from the GOSAT satellite (ACOS retrieval, filtered and bias-corrected) and in situ observations (individual flask and afternoon-average continuous observations) to estimate fluxes in 108 regions over 8-day intervals for the batch inversion and at 3° x 3.75° weekly for the variational system. Relationships between fluxes and atmospheric concentrations are derived consistently for the two inversion systems using the PCTM atmospheric transport model driven by meteorology from the MERRA reanalysis. We compare the posterior fluxes and uncertainties derived using different data sets and the two inversion approaches, and evaluate the posterior atmospheric concentrations against independent data including aircraft measurements. The optimized fluxes generally resemble those from other studies. For example, the results indicate that the terrestrial biosphere is a net CO2 sink, and a GOSAT-only inversion suggests a shift in the global sink from the tropics/south to the north relative to the prior and to an in-situ-only inversion. We also find a smaller terrestrial sink in higher-latitude northern regions in boreal summer of 2010 relative to 2009.

  4. An Inversion Analysis of Recent Variability in Natural CO2 Fluxes Using GOSAT and In Situ Observations

    NASA Technical Reports Server (NTRS)

    Wang, James S.; Kawa, S. Randolph; Collatz, G. James; Baker, David F.; Ott, Lesley

    2015-01-01

    About one-half of the global CO2 emissions from fossil fuel combustion and deforestation accumulates in the atmosphere, where it contributes to global warming. The rest is taken up by vegetation and the ocean. The precise contribution of the two sinks, and their location and year-to-year variability are, however, not well understood. We use two different approaches, batch Bayesian synthesis inversion and variational data assimilation, to deduce the global spatiotemporal distributions of CO2 fluxes during 2009-2010. One of our objectives is to assess different sources of uncertainties in inferred fluxes, including uncertainties in prior flux estimates and observations, and differences in inversion techniques. For prior constraints, we utilize fluxes and uncertainties from the CASA-GFED model of the terrestrial biosphere and biomass burning driven by satellite observations and interannually varying meteorology. We also use measurement-based ocean flux estimates and two sets of fixed fossil CO2 emissions. Here, our inversions incorporate column CO2 measurements from the GOSAT satellite (ACOS retrieval, filtered and bias-corrected) and in situ observations (individual flask and afternoon-average continuous observations) to estimate fluxes in 108 regions over 8-day intervals for the batch inversion and at 3 x 3.75 weekly for the variational system. Relationships between fluxes and atmospheric concentrations are derived consistently for the two inversion systems using the PCTM atmospheric transport model driven by meteorology from the MERRA reanalysis. We compare the posterior fluxes and uncertainties derived using different data sets and the two inversion approaches, and evaluate the posterior atmospheric concentrations against independent data including aircraft measurements. The optimized fluxes generally resemble those from other studies. For example, the results indicate that the terrestrial biosphere is a net CO2 sink, and a GOSAT-only inversion suggests a shift in the global sink from the tropics south to the north relative to the prior and to an in-situ-only inversion. We also find a smaller terrestrial sink in higher-latitude northern regions in boreal summer of 2010 relative to 2009.

  5. Do lab-derived distribution coefficient values of pesticides match distribution coefficient values determined from column and field-scale experiments? A critical analysis of relevant literature.

    PubMed

    Vereecken, H; Vanderborght, J; Kasteel, R; Spiteller, M; Schäffer, A; Close, M

    2011-01-01

    In this study, we analyzed sorption parameters for pesticides that were derived from batch and column or batch and field experiments. The batch experiments analyzed in this study were run with the same pesticide and soil as in the column and field experiments. We analyzed the relationship between the pore water velocity of the column and field experiments, solute residence times, and sorption parameters, such as the organic carbon normalized distribution coefficient ( ) and the mass exchange coefficient in kinetic models, as well as the predictability of sorption parameters from basic soil properties. The batch/column analysis included 38 studies with a total of 139 observations. The batch/field analysis included five studies, resulting in a dataset of 24 observations. For the batch/column data, power law relationships between pore water velocity, residence time, and sorption constants were derived. The unexplained variability in these equations was reduced, taking into account the saturation status and the packing status (disturbed-undisturbed) of the soil sample. A new regression equation was derived that allows estimating the values derived from column experiments using organic matter and bulk density with an value of 0.56. Regression analysis of the batch/column data showed that the relationship between batch- and column-derived values depends on the saturation status and packing of the soil column. Analysis of the batch/field data showed that as the batch-derived value becomes larger, field-derived values tend to be lower than the corresponding batch-derived values, and vice versa. The present dataset also showed that the variability in the ratio of batch- to column-derived value increases with increasing pore water velocity, with a maximum value approaching 3.5. American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America.

  6. Identifying and mitigating batch effects in whole genome sequencing data.

    PubMed

    Tom, Jennifer A; Reeder, Jens; Forrest, William F; Graham, Robert R; Hunkapiller, Julie; Behrens, Timothy W; Bhangale, Tushar R

    2017-07-24

    Large sample sets of whole genome sequencing with deep coverage are being generated, however assembling datasets from different sources inevitably introduces batch effects. These batch effects are not well understood and can be due to changes in the sequencing protocol or bioinformatics tools used to process the data. No systematic algorithms or heuristics exist to detect and filter batch effects or remove associations impacted by batch effects in whole genome sequencing data. We describe key quality metrics, provide a freely available software package to compute them, and demonstrate that identification of batch effects is aided by principal components analysis of these metrics. To mitigate batch effects, we developed new site-specific filters that identified and removed variants that falsely associated with the phenotype due to batch effect. These include filtering based on: a haplotype based genotype correction, a differential genotype quality test, and removing sites with missing genotype rate greater than 30% after setting genotypes with quality scores less than 20 to missing. This method removed 96.1% of unconfirmed genome-wide significant SNP associations and 97.6% of unconfirmed genome-wide significant indel associations. We performed analyses to demonstrate that: 1) These filters impacted variants known to be disease associated as 2 out of 16 confirmed associations in an AMD candidate SNP analysis were filtered, representing a reduction in power of 12.5%, 2) In the absence of batch effects, these filters removed only a small proportion of variants across the genome (type I error rate of 3%), and 3) in an independent dataset, the method removed 90.2% of unconfirmed genome-wide SNP associations and 89.8% of unconfirmed genome-wide indel associations. Researchers currently do not have effective tools to identify and mitigate batch effects in whole genome sequencing data. We developed and validated methods and filters to address this deficiency.

  7. Simulation of OSCM Concepts for HQ SACT

    DTIC Science & Technology

    2007-06-01

    effective method for creating understanding, identifying problems and developing solutions. • Simulation of a goal driven organization is a cost...effective method to visualize some aspects of the problem space Toolbox • The team used Extend™, a COTS product from Imagine That!® (http...Nations flow Model OSCM ATARES flow Batching A/C & Pallets Model ISAF Airbridge flow Flying and unbatching A/C Fleet Create resources Calculate flight

  8. BATCH-GE: Batch analysis of Next-Generation Sequencing data for genome editing assessment

    PubMed Central

    Boel, Annekatrien; Steyaert, Woutert; De Rocker, Nina; Menten, Björn; Callewaert, Bert; De Paepe, Anne; Coucke, Paul; Willaert, Andy

    2016-01-01

    Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions. Due to its tremendous progress in terms of speed, read length, throughput and cost, Next-Generation Sequencing (NGS) has been increasingly used for the analysis of CRISPR/Cas9 genome editing experiments. However, the current tools for genome editing assessment lack flexibility and fall short in the analysis of large amounts of NGS data. Therefore, we designed BATCH-GE, an easy-to-use bioinformatics tool for batch analysis of NGS-generated genome editing data, available from https://github.com/WouterSteyaert/BATCH-GE.git. BATCH-GE detects and reports indel mutations and other precise genome editing events and calculates the corresponding mutagenesis efficiencies for a large number of samples in parallel. Furthermore, this new tool provides flexibility by allowing the user to adapt a number of input variables. The performance of BATCH-GE was evaluated in two genome editing experiments, aiming to generate knock-out and knock-in zebrafish mutants. This tool will not only contribute to the evaluation of CRISPR/Cas9-based experiments, but will be of use in any genome editing experiment and has the ability to analyze data from every organism with a sequenced genome. PMID:27461955

  9. Optical Tracking Data Validation and Orbit Estimation for Sparse Observations of Satellites by the OWL-Net.

    PubMed

    Choi, Jin; Jo, Jung Hyun; Yim, Hong-Suh; Choi, Eun-Jung; Cho, Sungki; Park, Jang-Hyun

    2018-06-07

    An Optical Wide-field patroL-Network (OWL-Net) has been developed for maintaining Korean low Earth orbit (LEO) satellites' orbital ephemeris. The OWL-Net consists of five optical tracking stations. Brightness signals of reflected sunlight of the targets were detected by a charged coupled device (CCD). A chopper system was adopted for fast astrometric data sampling, maximum 50 Hz, within a short observation time. The astrometric accuracy of the optical observation data was validated with precise orbital ephemeris such as Consolidated Prediction File (CPF) data and precise orbit determination result with onboard Global Positioning System (GPS) data from the target satellite. In the optical observation simulation of the OWL-Net for 2017, an average observation span for a single arc of 11 LEO observation targets was about 5 min, while an average optical observation separation time was 5 h. We estimated the position and velocity with an atmospheric drag coefficient of LEO observation targets using a sequential-batch orbit estimation technique after multi-arc batch orbit estimation. Post-fit residuals for the multi-arc batch orbit estimation and sequential-batch orbit estimation were analyzed for the optical measurements and reference orbit (CPF and GPS data). The post-fit residuals with reference show few tens-of-meters errors for in-track direction for multi-arc batch and sequential-batch orbit estimation results.

  10. Future supply chains enabled by continuous processing--opportunities and challenges. May 20-21, 2014 Continuous Manufacturing Symposium.

    PubMed

    Srai, Jagjit Singh; Badman, Clive; Krumme, Markus; Futran, Mauricio; Johnston, Craig

    2015-03-01

    This paper examines the opportunities and challenges facing the pharmaceutical industry in moving to a primarily "continuous processing"-based supply chain. The current predominantly "large batch" and centralized manufacturing system designed for the "blockbuster" drug has driven a slow-paced, inventory heavy operating model that is increasingly regarded as inflexible and unsustainable. Indeed, new markets and the rapidly evolving technology landscape will drive more product variety, shorter product life-cycles, and smaller drug volumes, which will exacerbate an already unsustainable economic model. Future supply chains will be required to enhance affordability and availability for patients and healthcare providers alike despite the increased product complexity. In this more challenging supply scenario, we examine the potential for a more pull driven, near real-time demand-based supply chain, utilizing continuous processing where appropriate as a key element of a more "flow-through" operating model. In this discussion paper on future supply chain models underpinned by developments in the continuous manufacture of pharmaceuticals, we have set out; The significant opportunities to moving to a supply chain flow-through operating model, with substantial opportunities in inventory reduction, lead-time to patient, and radically different product assurance/stability regimes. Scenarios for decentralized production models producing a greater variety of products with enhanced volume flexibility. Production, supply, and value chain footprints that are radically different from today's monolithic and centralized batch manufacturing operations. Clinical trial and drug product development cost savings that support more rapid scale-up and market entry models with early involvement of SC designers within New Product Development. The major supply chain and industrial transformational challenges that need to be addressed. The paper recognizes that although current batch operational performance in pharma is far from optimal and not necessarily an appropriate end-state benchmark for batch technology, the adoption of continuous supply chain operating models underpinned by continuous production processing, as full or hybrid solutions in selected product supply chains, can support industry transformations to deliver right-first-time quality at substantially lower inventory profiles. © 2015 Wiley Periodicals, Inc. and the American Pharmacists Association.

  11. Bioelectrochemical conversion of CO2 to chemicals: CO2 as a next generation feedstock for electricity-driven bioproduction in batch and continuous modes.

    PubMed

    Bajracharya, Suman; Vanbroekhoven, Karolien; Buisman, Cees J N; Strik, David P B T B; Pant, Deepak

    2017-09-21

    The recent concept of microbial electrosynthesis (MES) has evolved as an electricity-driven production technology for chemicals from low-value carbon dioxide (CO 2 ) using micro-organisms as biocatalysts. MES from CO 2 comprises bioelectrochemical reduction of CO 2 to multi-carbon organic compounds using the reducing equivalents produced at the electrically-polarized cathode. The use of CO 2 as a feedstock for chemicals is gaining much attention, since CO 2 is abundantly available and its use is independent of the food supply chain. MES based on CO 2 reduction produces acetate as a primary product. In order to elucidate the performance of the bioelectrochemical CO 2 reduction process using different operation modes (batch vs. continuous), an investigation was carried out using a MES system with a flow-through biocathode supplied with 20 : 80 (v/v) or 80 : 20 (v/v) CO 2  : N 2 gas. The highest acetate production rate of 149 mg L -1 d -1 was observed with a 3.1 V applied cell-voltage under batch mode. While running in continuous mode, high acetate production was achieved with a maximum rate of 100 mg L -1 d -1 . In the continuous mode, the acetate production was not sustained over long-term operation, likely due to insufficient microbial biocatalyst retention within the biocathode compartment (i.e. suspended micro-organisms were washed out of the system). Restarting batch mode operations resulted in a renewed production of acetate. This showed an apparent domination of suspended biocatalysts over the attached (biofilm forming) biocatalysts. Long term CO 2 reduction at the biocathode resulted in the accumulation of acetate, and more reduced compounds like ethanol and butyrate were also formed. Improvements in the production rate and different biomass retention strategies (e.g. selecting for biofilm forming micro-organisms) should be investigated to enable continuous biochemical production from CO 2 using MES. Certainly, other process optimizations will be required to establish MES as an innovative sustainable technology for manufacturing biochemicals from CO 2 as a next generation feedstock.

  12. Improving tablet coating robustness by selecting critical process parameters from retrospective data.

    PubMed

    Galí, A; García-Montoya, E; Ascaso, M; Pérez-Lozano, P; Ticó, J R; Miñarro, M; Suñé-Negre, J M

    2016-09-01

    Although tablet coating processes are widely used in the pharmaceutical industry, they often lack adequate robustness. Up-scaling can be challenging as minor changes in parameters can lead to varying quality results. To select critical process parameters (CPP) using retrospective data of a commercial product and to establish a design of experiments (DoE) that would improve the robustness of the coating process. A retrospective analysis of data from 36 commercial batches. Batches were selected based on the quality results generated during batch release, some of which revealed quality deviations concerning the appearance of the coated tablets. The product is already marketed and belongs to the portfolio of a multinational pharmaceutical company. The Statgraphics 5.1 software was used for data processing to determine critical process parameters in order to propose new working ranges. This study confirms that it is possible to determine the critical process parameters and create design spaces based on retrospective data of commercial batches. This type of analysis is thus converted into a tool to optimize the robustness of existing processes. Our results show that a design space can be established with minimum investment in experiments, since current commercial batch data are processed statistically.

  13. Batch process fault detection and identification based on discriminant global preserving kernel slow feature analysis.

    PubMed

    Zhang, Hanyuan; Tian, Xuemin; Deng, Xiaogang; Cao, Yuping

    2018-05-16

    As an attractive nonlinear dynamic data analysis tool, global preserving kernel slow feature analysis (GKSFA) has achieved great success in extracting the high nonlinearity and inherently time-varying dynamics of batch process. However, GKSFA is an unsupervised feature extraction method and lacks the ability to utilize batch process class label information, which may not offer the most effective means for dealing with batch process monitoring. To overcome this problem, we propose a novel batch process monitoring method based on the modified GKSFA, referred to as discriminant global preserving kernel slow feature analysis (DGKSFA), by closely integrating discriminant analysis and GKSFA. The proposed DGKSFA method can extract discriminant feature of batch process as well as preserve global and local geometrical structure information of observed data. For the purpose of fault detection, a monitoring statistic is constructed based on the distance between the optimal kernel feature vectors of test data and normal data. To tackle the challenging issue of nonlinear fault variable identification, a new nonlinear contribution plot method is also developed to help identifying the fault variable after a fault is detected, which is derived from the idea of variable pseudo-sample trajectory projection in DGKSFA nonlinear biplot. Simulation results conducted on a numerical nonlinear dynamic system and the benchmark fed-batch penicillin fermentation process demonstrate that the proposed process monitoring and fault diagnosis approach can effectively detect fault and distinguish fault variables from normal variables. Copyright © 2018 ISA. Published by Elsevier Ltd. All rights reserved.

  14. User-driven integrated software lives: ``Paleomag'' paleomagnetics analysis on the Macintosh

    NASA Astrophysics Data System (ADS)

    Jones, Craig H.

    2002-12-01

    "PaleoMag," a paleomagnetics analysis package originally developed for the Macintosh operating system in 1988, allows examination of demagnetization of individual samples and analysis of directional data from collections of samples. Prior to recent reinvigorated development of the software for both Macintosh and Windows, it was widely used despite not running properly on machines and operating systems sold after 1995. This somewhat surprising situation demonstrates that there is a continued need for integrated analysis software within the earth sciences, in addition to well-developed scripting and batch-mode software. One distinct advantage of software like PaleoMag is in the ability to combine quality control with analysis within a unique graphical environment. Because such demands are frequent within the earth sciences, means of nurturing the development of similar software should be found.

  15. Applicability of the Monocyte Activation Test (MAT) in the quality control of the 17DD yellow fever vaccine.

    PubMed

    de Mattos, Katherine Antunes; Navega, Elaine Cristina Azevedo; Silva, Vitor Fernandes; Almeida, Alessandra Santos; da Silva, Cristiane Caldeira; Presgrave, Octavio Augusto França; Junior, Daniel da Silva Guedes; Delgado, Isabella Fernandes

    2018-03-01

    The need for alternatives to animal use in pyrogen testing has been driven by the Three Rs concept. This has resulted in the inclusion of the monocyte activation test (MAT) in the European Pharmacopoeia, 2010. However, some technical and regulatory obstacles must be overcome to ensure the effective implementation of the MAT by the industry, especially for the testing of biological products. The yellow fever (YF) vaccine (17DD-YFV) was chosen for evaluation in this study, in view of: a) the 2016-2018 outbreak of YF in Brazil; b) the increase in demand for 17DD-YFV doses; c) the complex production process with live attenuated virus; d) the presence of possible test interference factors, such as residual process components (e.g. ovalbumin); and e) the need for the investigation of other pyrogens that are not detectable by the methods prescribed in the YF vaccine monograph. The product-specific testing was carried out by using cryopreserved and fresh whole blood, and IL-6 and IL-1β levels were used as the marker readouts. After assessing the applicability of the MAT on a 1:10 dilution of 17DD-YFV, endotoxin and non-endotoxin pyrogens were quantified in spiked batches, by using the lipopolysaccharide and lipoteichoic acid standards, respectively. The quantitative analysis demonstrated the correlation between the MAT and the Limulus amoebocyte lysate (LAL) assays, with respect to the limits of endotoxin recovery in spiked batches and the detection of no pyrogenic contamination in commercial batches of 17DD-YFV. The data demonstrated the applicability of the MAT for 17DD-YFV pyrogen testing, and as an alternative method that can contribute to biological quality control studies. 2018 FRAME.

  16. Multivariate statistical process control (MSPC) using Raman spectroscopy for in-line culture cell monitoring considering time-varying batches synchronized with correlation optimized warping (COW).

    PubMed

    Liu, Ya-Juan; André, Silvère; Saint Cristau, Lydia; Lagresle, Sylvain; Hannas, Zahia; Calvosa, Éric; Devos, Olivier; Duponchel, Ludovic

    2017-02-01

    Multivariate statistical process control (MSPC) is increasingly popular as the challenge provided by large multivariate datasets from analytical instruments such as Raman spectroscopy for the monitoring of complex cell cultures in the biopharmaceutical industry. However, Raman spectroscopy for in-line monitoring often produces unsynchronized data sets, resulting in time-varying batches. Moreover, unsynchronized data sets are common for cell culture monitoring because spectroscopic measurements are generally recorded in an alternate way, with more than one optical probe parallelly connecting to the same spectrometer. Synchronized batches are prerequisite for the application of multivariate analysis such as multi-way principal component analysis (MPCA) for the MSPC monitoring. Correlation optimized warping (COW) is a popular method for data alignment with satisfactory performance; however, it has never been applied to synchronize acquisition time of spectroscopic datasets in MSPC application before. In this paper we propose, for the first time, to use the method of COW to synchronize batches with varying durations analyzed with Raman spectroscopy. In a second step, we developed MPCA models at different time intervals based on the normal operation condition (NOC) batches synchronized by COW. New batches are finally projected considering the corresponding MPCA model. We monitored the evolution of the batches using two multivariate control charts based on Hotelling's T 2 and Q. As illustrated with results, the MSPC model was able to identify abnormal operation condition including contaminated batches which is of prime importance in cell culture monitoring We proved that Raman-based MSPC monitoring can be used to diagnose batches deviating from the normal condition, with higher efficacy than traditional diagnosis, which would save time and money in the biopharmaceutical industry. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Unobtrusive integration of data management with fMRI analysis.

    PubMed

    Poliakov, Andrew V; Hertzenberg, Xenia; Moore, Eider B; Corina, David P; Ojemann, George A; Brinkley, James F

    2007-01-01

    This note describes a software utility, called X-batch which addresses two pressing issues typically faced by functional magnetic resonance imaging (fMRI) neuroimaging laboratories (1) analysis automation and (2) data management. The first issue is addressed by providing a simple batch mode processing tool for the popular SPM software package (http://www.fil.ion. ucl.ac.uk/spm/; Welcome Department of Imaging Neuroscience, London, UK). The second is addressed by transparently recording metadata describing all aspects of the batch job (e.g., subject demographics, analysis parameters, locations and names of created files, date and time of analysis, and so on). These metadata are recorded as instances of an extended version of the Protégé-based Experiment Lab Book ontology created by the Dartmouth fMRI Data Center. The resulting instantiated ontology provides a detailed record of all fMRI analyses performed, and as such can be part of larger systems for neuroimaging data management, sharing, and visualization. The X-batch system is in use in our own fMRI research, and is available for download at http://X-batch.sourceforge.net/.

  18. Removal of batch effects using distribution-matching residual networks.

    PubMed

    Shaham, Uri; Stanton, Kelly P; Zhao, Jun; Li, Huamin; Raddassi, Khadir; Montgomery, Ruth; Kluger, Yuval

    2017-08-15

    Sources of variability in experimentally derived data include measurement error in addition to the physical phenomena of interest. This measurement error is a combination of systematic components, originating from the measuring instrument and random measurement errors. Several novel biological technologies, such as mass cytometry and single-cell RNA-seq (scRNA-seq), are plagued with systematic errors that may severely affect statistical analysis if the data are not properly calibrated. We propose a novel deep learning approach for removing systematic batch effects. Our method is based on a residual neural network, trained to minimize the Maximum Mean Discrepancy between the multivariate distributions of two replicates, measured in different batches. We apply our method to mass cytometry and scRNA-seq datasets, and demonstrate that it effectively attenuates batch effects. our codes and data are publicly available at https://github.com/ushaham/BatchEffectRemoval.git. yuval.kluger@yale.edu. Supplementary data are available at Bioinformatics online. © The Author (2017). Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com

  19. The operable modeling of simultaneous saccharification and fermentation of ethanol production from cellulose.

    PubMed

    Shen, Jiacheng; Agblevor, Foster A

    2010-03-01

    An operable batch model of simultaneous saccharification and fermentation (SSF) for ethanol production from cellulose has been developed. The model includes four ordinary differential equations that describe the changes of cellobiose, glucose, yeast, and ethanol concentrations with respect to time. These equations were used to simulate the experimental data of the four main components in the SSF process of ethanol production from microcrystalline cellulose (Avicel PH101). The model parameters at 95% confidence intervals were determined by a MATLAB program based on the batch experimental data of the SSF. Both experimental data and model simulations showed that the cell growth was the rate-controlling step at the initial period in a series of reactions of cellulose to ethanol, and later, the conversion of cellulose to cellobiose controlled the process. The batch model was extended to the continuous and fed-batch operating models. For the continuous operation in the SSF, the ethanol productivities increased with increasing dilution rate, until a maximum value was attained, and rapidly decreased as the dilution rate approached the washout point. The model also predicted a relatively high ethanol mass for the fed-batch operation than the batch operation.

  20. Catalytic wet oxidation of phenol in a trickle bed reactor over a Pt/TiO2 catalyst.

    PubMed

    Maugans, Clayton B; Akgerman, Aydin

    2003-01-01

    Catalytic wet oxidation of phenol was studied in a batch and a trickle bed reactor using 4.45% Pt/TiO2 catalyst in the temperature range 150-205 degrees C. Kinetic data were obtained from batch reactor studies and used to model the reaction kinetics for phenol disappearance and for total organic carbon disappearance. Trickle bed experiments were then performed to generate data from a heterogeneous flow reactor. Catalyst deactivation was observed in the trickle bed reactor, although the exact cause was not determined. Deactivation was observed to linearly increase with the cumulative amount of phenol that had passed over the catalyst bed. Trickle bed reactor modeling was performed using a three-phase heterogeneous model. Model parameters were determined from literature correlations, batch derived kinetic data, and trickle bed derived catalyst deactivation data. The model equations were solved using orthogonal collocations on finite elements. Trickle bed performance was successfully predicted using the batch derived kinetic model and the three-phase reactor model. Thus, using the kinetics determined from limited data in the batch mode, it is possible to predict continuous flow multiphase reactor performance.

  1. Modeling of Fusarium redolens Dzf2 mycelial growth kinetics and optimal fed-batch fermentation for beauvericin production.

    PubMed

    Xu, Li-Jian; Liu, Yuan-Shuai; Zhou, Li-Gang; Wu, Jian-Yong

    2011-09-01

    Beauvericin (BEA) is a cyclic hexadepsipeptide mycotoxin with notable phytotoxic and insecticidal activities. Fusarium redolens Dzf2 is a highly BEA-producing fungus isolated from a medicinal plant. The aim of the current study was to develop a simple and valid kinetic model for F. redolens Dzf2 mycelial growth and the optimal fed-batch operation for efficient BEA production. A modified Monod model with substrate (glucose) and product (BEA) inhibition was constructed based on the culture characteristics of F. redolens Dzf2 mycelia in a liquid medium. Model parameters were derived by simulation of the experimental data from batch culture. The model fitted closely with the experimental data over 20-50 g l(-1) glucose concentration range in batch fermentation. The kinetic model together with the stoichiometric relationships for biomass, substrate and product was applied to predict the optimal feeding scheme for fed-batch fermentation, leading to 54% higher BEA yield (299 mg l(-1)) than in the batch culture (194 mg l(-1)). The modified Monod model incorporating substrate and product inhibition was proven adequate for describing the growth kinetics of F. redolens Dzf2 mycelial culture at suitable but not excessive initial glucose levels in batch and fed-batch cultures.

  2. Batch Effect Confounding Leads to Strong Bias in Performance Estimates Obtained by Cross-Validation

    PubMed Central

    Delorenzi, Mauro

    2014-01-01

    Background With the large amount of biological data that is currently publicly available, many investigators combine multiple data sets to increase the sample size and potentially also the power of their analyses. However, technical differences (“batch effects”) as well as differences in sample composition between the data sets may significantly affect the ability to draw generalizable conclusions from such studies. Focus The current study focuses on the construction of classifiers, and the use of cross-validation to estimate their performance. In particular, we investigate the impact of batch effects and differences in sample composition between batches on the accuracy of the classification performance estimate obtained via cross-validation. The focus on estimation bias is a main difference compared to previous studies, which have mostly focused on the predictive performance and how it relates to the presence of batch effects. Data We work on simulated data sets. To have realistic intensity distributions, we use real gene expression data as the basis for our simulation. Random samples from this expression matrix are selected and assigned to group 1 (e.g., ‘control’) or group 2 (e.g., ‘treated’). We introduce batch effects and select some features to be differentially expressed between the two groups. We consider several scenarios for our study, most importantly different levels of confounding between groups and batch effects. Methods We focus on well-known classifiers: logistic regression, Support Vector Machines (SVM), k-nearest neighbors (kNN) and Random Forests (RF). Feature selection is performed with the Wilcoxon test or the lasso. Parameter tuning and feature selection, as well as the estimation of the prediction performance of each classifier, is performed within a nested cross-validation scheme. The estimated classification performance is then compared to what is obtained when applying the classifier to independent data. PMID:24967636

  3. Batch Model for Batched Timestamps Data Analysis with Application to the SSA Disability Program

    PubMed Central

    Yue, Qingqi; Yuan, Ao; Che, Xuan; Huynh, Minh; Zhou, Chunxiao

    2016-01-01

    The Office of Disability Adjudication and Review (ODAR) is responsible for holding hearings, issuing decisions, and reviewing appeals as part of the Social Security Administration’s disability determining process. In order to control and process cases, the ODAR has established a Case Processing and Management System (CPMS) to record management information since December 2003. The CPMS provides a detailed case status history for each case. Due to the large number of appeal requests and limited resources, the number of pending claims at ODAR was over one million cases by March 31, 2015. Our National Institutes of Health (NIH) team collaborated with SSA and developed a Case Status Change Model (CSCM) project to meet the ODAR’s urgent need of reducing backlogs and improve hearings and appeals process. One of the key issues in our CSCM project is to estimate the expected service time and its variation for each case status code. The challenge is that the systems recorded job departure times may not be the true job finished times. As the CPMS timestamps data of case status codes showed apparent batch patterns, we proposed a batch model and applied the constrained least squares method to estimate the mean service times and the variances. We also proposed a batch search algorithm to determine the optimal batch partition, as no batch partition was given in the real data. Simulation studies were conducted to evaluate the performance of the proposed methods. Finally, we applied the method to analyze a real CPMS data from ODAR/SSA. PMID:27747132

  4. Acceptance Test Data for the AGR-5/6/7 Irradiation Test Fuel Composite Defective IPyC Fraction and Pyrocarbon Anisotropy

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Helmreich, Grant W.; Hunn, John D.; Skitt, Darren J.

    Coated particle composite J52R-16-98005 was produced by Babcock and Wilcox Technologies (BWXT) as fuel for the Advanced Gas Reactor Fuel Development and Qualification (AGR) Program’s AGR-5/6/7 irradiation test in the Idaho National Laboratory (INL) Advanced Test Reactor (ATR). This composite was comprised of four coated particle fuel batches J52O-16-93165B (26%), 93168B (26%), 93169B (24%), and 93170B (24%), chosen based on the Quality Control (QC) data acquired for each individual candidate AGR-5/6/7 batch. Each batch was coated in a 150-mm-diameter production-scale fluidized-bed chemical vapor deposition (CVD) furnace. Tristructural isotropic (TRISO) coatings were deposited on 425-μm-nominal-diameter spherical kernels from BWXT Lot J52R-16-69317more » containing a mixture of 15.5%-enriched uranium carbide and uranium oxide (UCO). The TRISO coatings consisted of four consecutive CVD layers: a ~50% dense carbon buffer layer with 100-μm-nominal thickness, a dense inner pyrolytic carbon (IPyC) layer with 40-μm-nominal thickness, a silicon carbide (SiC) layer with 35-μm-nominal thickness, and a dense outer pyrolytic carbon (OPyC) layer with 40-μm-nominal thickness. The TRISO-coated particle batches were sieved to upgrade the particles by removing over-sized and under-sized material, and the upgraded batches were designated by appending the letter A to the end of the batch number (e.g., 93165A). Secondary upgrading by sieving was performed on the A-designated batches to remove particles with missing or very-thin buffer layers that were identified during previous analysis of the individual batches for defective IPyC, as reported in the acceptance test data report for the AGR-5/6/7 production batches [Hunn et al. 2017]. The additionally-upgraded batches were designated by appending the letter B to the end of the batch number (e.g., 93165B).« less

  5. Gas-driven microturbine

    DOEpatents

    Sniegowski, Jeffrey J.; Rodgers, Murray S.; McWhorter, Paul J.; Aeschliman, Daniel P.; Miller, William M.

    2002-01-01

    A microturbine fabricated by a three-level semiconductor batch-fabrication process based on polysilicon surface-micromachining. The microturbine comprises microelectromechanical elements formed from three polysilicon multi-layer surfaces applied to a silicon substrate. Interleaving sacrificial oxide layers provides electrical and physical isolation, and selective etching of both the sacrificial layers and the polysilicon layers allows formation of individual mechanical and electrical elements as well as the required space for necessary movement of rotating turbine parts and linear elements.

  6. Acceptance Test Data for BWXT Coated Particle Batches 93172B and 93173B—Defective IPyC and Pyrocarbon Anisotropy

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hunn, John D.; Helmreich, Grant W.; Dyer, John A.

    Coated particle batches J52O-16-93172B and J52O-16-93173B were produced by Babcock and Wilcox Technologies (BWXT) as part of the production campaign for the Advanced Gas Reactor Fuel Development and Qualification (AGR) Program’s AGR-5/6/7 irradiation test in the Idaho National Laboratory (INL) Advanced Test Reactor (ATR), but were not used in the final fuel composite. However, these batches may be used as demonstration production-scale coated particle fuel for other experiments. Each batch was coated in a 150-mm-diameter production-scale fluidized-bed chemical vapor deposition (CVD) furnace. Tristructural isotropic (TRISO) coatings were deposited on 425-μm-nominal-diameter spherical kernels from BWXT lot J52R-16-69317 containing a mixture ofmore » 15.5%-enriched uranium carbide and uranium oxide (UCO). The TRISO coatings consisted of four consecutive CVD layers: a ~50% dense carbon buffer layer with 100-μm-nominal thickness, a dense inner pyrolytic carbon (IPyC) layer with 40-μm-nominal thickness, a silicon carbide (SiC) layer with 35-μm-nominal thickness, and a dense outer pyrolytic carbon (OPyC) layer with 40-μm-nominal thickness. The TRISO-coated particle batches were sieved to upgrade the particles by removing over-sized and under-sized material, and the upgraded batches were designated by appending the letter A to the end of the batch number (e.g., 93172A). Secondary upgrading by sieving was performed on the A-designated batches to remove particles with missing or very-thin buffer layers that were identified during previous analysis of the individual batches for defective IPyC, as reported in the acceptance test data report for the AGR-5/6/7 production batches [Hunn et al. 2017b]. The additionally-upgraded batches were designated by appending the letter B to the end of the batch number (e.g., 93172B).« less

  7. Application of a continuous twin screw-driven process for dilute acid pretreatment of rape straw.

    PubMed

    Choi, Chang Ho; Oh, Kyeong Keun

    2012-04-01

    Rape straw, a processing residue generated from the bio-oil industry, was used as a model biomass for application of continuous twin screw-driven dilute acid pretreatment. The screw rotation speed and feeding rate were adjusted to 19.7rpm and 0.5g/min, respectively to maintain a residence time of 7.2min in the reaction zone, respectively. The sulfuric acid concentration was 3.5wt% and the reaction temperature was 165°C. The enzymatic digestibility of the glucan in the pretreated solids was 70.9%. The continuous process routinely gave around 28.8% higher yield for glucan digestibility than did the batch processing method. Copyright © 2012 Elsevier Ltd. All rights reserved.

  8. Analyzing data flows of WLCG jobs at batch job level

    NASA Astrophysics Data System (ADS)

    Kuehn, Eileen; Fischer, Max; Giffels, Manuel; Jung, Christopher; Petzold, Andreas

    2015-05-01

    With the introduction of federated data access to the workflows of WLCG, it is becoming increasingly important for data centers to understand specific data flows regarding storage element accesses, firewall configurations, as well as the scheduling of batch jobs themselves. As existing batch system monitoring and related system monitoring tools do not support measurements at batch job level, a new tool has been developed and put into operation at the GridKa Tier 1 center for monitoring continuous data streams and characteristics of WLCG jobs and pilots. Long term measurements and data collection are in progress. These measurements already have been proven to be useful analyzing misbehaviors and various issues. Therefore we aim for an automated, realtime approach for anomaly detection. As a requirement, prototypes for standard workflows have to be examined. Based on measurements of several months, different features of HEP jobs are evaluated regarding their effectiveness for data mining approaches to identify these common workflows. The paper will introduce the actual measurement approach and statistics as well as the general concept and first results classifying different HEP job workflows derived from the measurements at GridKa.

  9. A comparison of abundance estimates from extended batch-marking and Jolly–Seber-type experiments

    PubMed Central

    Cowen, Laura L E; Besbeas, Panagiotis; Morgan, Byron J T; Schwarz, Carl J

    2014-01-01

    Little attention has been paid to the use of multi-sample batch-marking studies, as it is generally assumed that an individual's capture history is necessary for fully efficient estimates. However, recently, Huggins et al. (2010) present a pseudo-likelihood for a multi-sample batch-marking study where they used estimating equations to solve for survival and capture probabilities and then derived abundance estimates using a Horvitz–Thompson-type estimator. We have developed and maximized the likelihood for batch-marking studies. We use data simulated from a Jolly–Seber-type study and convert this to what would have been obtained from an extended batch-marking study. We compare our abundance estimates obtained from the Crosbie–Manly–Arnason–Schwarz (CMAS) model with those of the extended batch-marking model to determine the efficiency of collecting and analyzing batch-marking data. We found that estimates of abundance were similar for all three estimators: CMAS, Huggins, and our likelihood. Gains are made when using unique identifiers and employing the CMAS model in terms of precision; however, the likelihood typically had lower mean square error than the pseudo-likelihood method of Huggins et al. (2010). When faced with designing a batch-marking study, researchers can be confident in obtaining unbiased abundance estimators. Furthermore, they can design studies in order to reduce mean square error by manipulating capture probabilities and sample size. PMID:24558576

  10. Efficient provisioning for multi-core applications with LSF

    NASA Astrophysics Data System (ADS)

    Dal Pra, Stefano

    2015-12-01

    Tier-1 sites providing computing power for HEP experiments are usually tightly designed for high throughput performances. This is pursued by reducing the variety of supported use cases and tuning for performances those ones, the most important of which have been that of singlecore jobs. Moreover, the usual workload is saturation: each available core in the farm is in use and there are queued jobs waiting for their turn to run. Enabling multi-core jobs thus requires dedicating a number of hosts where to run, and waiting for them to free the needed number of cores. This drain-time introduces a loss of computing power driven by the number of unusable empty cores. As an increasing demand for multi-core capable resources have emerged, a Task Force have been constituted in WLCG, with the goal to define a simple and efficient multi-core resource provisioning model. This paper details the work done at the INFN Tier-1 to enable multi-core support for the LSF batch system, with the intent of reducing to the minimum the average number of unused cores. The adopted strategy has been that of dedicating to multi-core a dynamic set of nodes, whose dimension is mainly driven by the number of pending multi-core requests and fair-share priority of the submitting user. The node status transition, from single to multi core et vice versa, is driven by a finite state machine which is implemented in a custom multi-core director script, running in the cluster. After describing and motivating both the implementation and the details specific to the LSF batch system, results about performance are reported. Factors having positive and negative impact on the overall efficiency are discussed and solutions to reduce at most the negative ones are proposed.

  11. Continuous Manufacturing in Pharmaceutical Process Development and Manufacturing.

    PubMed

    Burcham, Christopher L; Florence, Alastair J; Johnson, Martin D

    2018-06-07

    The pharmaceutical industry has found new applications for the use of continuous processing for the manufacture of new therapies currently in development. The transformation has been encouraged by regulatory bodies as well as driven by cost reduction, decreased development cycles, access to new chemistries not practical in batch, improved safety, flexible manufacturing platforms, and improved product quality assurance. The transformation from batch to continuous manufacturing processing is the focus of this review. The review is limited to small, chemically synthesized organic molecules and encompasses the manufacture of both active pharmaceutical ingredients (APIs) and the subsequent drug product. Continuous drug product is currently used in approved processes. A few examples of production of APIs under current good manufacturing practice conditions using continuous processing steps have been published in the past five years, but they are lagging behind continuous drug product with respect to regulatory filings.

  12. U.S.-MEXICO BORDER PROGRAM ARIZONA BORDER STUDY--STANDARD OPERATING PROCEDURE FOR BATCHING OF LABORATORY DATA (UA-C-7.0)

    EPA Science Inventory

    The purpose of this SOP is to describe the steps involved in batching the physical laboratory data forms generated by the Arizona Border Study and slated for data entry. It applies to all physical laboratory data forms entered for this study. This procedure was followed to ensu...

  13. NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR BATCHING OF LAB DATA (UA-C-7.0)

    EPA Science Inventory

    The purpose of this SOP is to describe the steps involved in batching the physical laboratory data forms generated by NHEXAS Arizona and slated for data entry at the primary NHEXAS Arizona office. It applies to all physical laboratory data forms entered at this site. This proced...

  14. Interactive Parallel Data Analysis within Data-Centric Cluster Facilities using the IPython Notebook

    NASA Astrophysics Data System (ADS)

    Pascoe, S.; Lansdowne, J.; Iwi, A.; Stephens, A.; Kershaw, P.

    2012-12-01

    The data deluge is making traditional analysis workflows for many researchers obsolete. Support for parallelism within popular tools such as matlab, IDL and NCO is not well developed and rarely used. However parallelism is necessary for processing modern data volumes on a timescale conducive to curiosity-driven analysis. Furthermore, for peta-scale datasets such as the CMIP5 archive, it is no longer practical to bring an entire dataset to a researcher's workstation for analysis, or even to their institutional cluster. Therefore, there is an increasing need to develop new analysis platforms which both enable processing at the point of data storage and which provides parallelism. Such an environment should, where possible, maintain the convenience and familiarity of our current analysis environments to encourage curiosity-driven research. We describe how we are combining the interactive python shell (IPython) with our JASMIN data-cluster infrastructure. IPython has been specifically designed to bridge the gap between the HPC-style parallel workflows and the opportunistic curiosity-driven analysis usually carried out using domain specific languages and scriptable tools. IPython offers a web-based interactive environment, the IPython notebook, and a cluster engine for parallelism all underpinned by the well-respected Python/Scipy scientific programming stack. JASMIN is designed to support the data analysis requirements of the UK and European climate and earth system modeling community. JASMIN, with its sister facility CEMS focusing the earth observation community, has 4.5 PB of fast parallel disk storage alongside over 370 computing cores provide local computation. Through the IPython interface to JASMIN, users can make efficient use of JASMIN's multi-core virtual machines to perform interactive analysis on all cores simultaneously or can configure IPython clusters across multiple VMs. Larger-scale clusters can be provisioned through JASMIN's batch scheduling system. Outputs can be summarised and visualised using the full power of Python's many scientific tools, including Scipy, Matplotlib, Pandas and CDAT. This rich user experience is delivered through the user's web browser; maintaining the interactive feel of a workstation-based environment with the parallel power of a remote data-centric processing facility.

  15. Correcting for intra-experiment variation in Illumina BeadChip data is necessary to generate robust gene-expression profiles.

    PubMed

    Kitchen, Robert R; Sabine, Vicky S; Sims, Andrew H; Macaskill, E Jane; Renshaw, Lorna; Thomas, Jeremy S; van Hemert, Jano I; Dixon, J Michael; Bartlett, John M S

    2010-02-24

    Microarray technology is a popular means of producing whole genome transcriptional profiles, however high cost and scarcity of mRNA has led many studies to be conducted based on the analysis of single samples. We exploit the design of the Illumina platform, specifically multiple arrays on each chip, to evaluate intra-experiment technical variation using repeated hybridisations of universal human reference RNA (UHRR) and duplicate hybridisations of primary breast tumour samples from a clinical study. A clear batch-specific bias was detected in the measured expressions of both the UHRR and clinical samples. This bias was found to persist following standard microarray normalisation techniques. However, when mean-centering or empirical Bayes batch-correction methods (ComBat) were applied to the data, inter-batch variation in the UHRR and clinical samples were greatly reduced. Correlation between replicate UHRR samples improved by two orders of magnitude following batch-correction using ComBat (ranging from 0.9833-0.9991 to 0.9997-0.9999) and increased the consistency of the gene-lists from the duplicate clinical samples, from 11.6% in quantile normalised data to 66.4% in batch-corrected data. The use of UHRR as an inter-batch calibrator provided a small additional benefit when used in conjunction with ComBat, further increasing the agreement between the two gene-lists, up to 74.1%. In the interests of practicalities and cost, these results suggest that single samples can generate reliable data, but only after careful compensation for technical bias in the experiment. We recommend that investigators appreciate the propensity for such variation in the design stages of a microarray experiment and that the use of suitable correction methods become routine during the statistical analysis of the data.

  16. Correcting for intra-experiment variation in Illumina BeadChip data is necessary to generate robust gene-expression profiles

    PubMed Central

    2010-01-01

    Background Microarray technology is a popular means of producing whole genome transcriptional profiles, however high cost and scarcity of mRNA has led many studies to be conducted based on the analysis of single samples. We exploit the design of the Illumina platform, specifically multiple arrays on each chip, to evaluate intra-experiment technical variation using repeated hybridisations of universal human reference RNA (UHRR) and duplicate hybridisations of primary breast tumour samples from a clinical study. Results A clear batch-specific bias was detected in the measured expressions of both the UHRR and clinical samples. This bias was found to persist following standard microarray normalisation techniques. However, when mean-centering or empirical Bayes batch-correction methods (ComBat) were applied to the data, inter-batch variation in the UHRR and clinical samples were greatly reduced. Correlation between replicate UHRR samples improved by two orders of magnitude following batch-correction using ComBat (ranging from 0.9833-0.9991 to 0.9997-0.9999) and increased the consistency of the gene-lists from the duplicate clinical samples, from 11.6% in quantile normalised data to 66.4% in batch-corrected data. The use of UHRR as an inter-batch calibrator provided a small additional benefit when used in conjunction with ComBat, further increasing the agreement between the two gene-lists, up to 74.1%. Conclusion In the interests of practicalities and cost, these results suggest that single samples can generate reliable data, but only after careful compensation for technical bias in the experiment. We recommend that investigators appreciate the propensity for such variation in the design stages of a microarray experiment and that the use of suitable correction methods become routine during the statistical analysis of the data. PMID:20181233

  17. A preliminary evaluation of a reusable digital sterilization indicator prototype.

    PubMed

    Puttaiah, R; Griggs, J; D'Onofrio, M

    2014-09-01

    Sterilization of critical and semicritical instruments used in patient care must undergo a terminal process of sterilization. Use of chemical and physical indicators are important in providing information on the sterilizer's performance during each cycle. Regular and periodic monitoring of sterilizers using biological indicators is necessary in periodically validating performance of sterilizers. Data loggers or independent digital parametric indicators are innovative devices that provide more information than various classes chemical indicators. In this study we evaluated a prototype of an independent digital parametric indicator's use in autoclaves. The purpose of this study was to evaluate the performance of an independent digital indicator/data logger prototype (DS1922F) that could be used for multiple cycles within an autoclave.MG Materials and methods: Three batches of the DS1922F (150 samples) were used in this study that was conducted in a series. The first batch was challenged with 300 sterilization cycles within an autoclave and the data loggers evaluated to study failures and the reason for failure, make corrections and improve the prototype design. After changes made based on studying the first batch, the second batch of the prototype (150 samples) were challenged once again with 300 sterilization cycles within an autoclave and failure studied again in further improvement of the prototype. The final batch (3rd batch) of the prototype (150 samples) was challenged again but with 600 cycles to see how long they would last. Kaplan-Meier survival analysis analyses of all three batches was conducted (α = 0.05) and failed samples qualitatively studied in understanding the variables involved in the failure of the prototype, and in improving quality. Each tested batch provided crucial information on device failure and helped in improvement of the prototype. Mean lifetime survival of the final batch (Batch 3) of prototype was 498 (480, 516) sterilization cycles in an autoclave. In this study, the final batch of the DS1922F prototype data logger was found to be robust in withstanding the challenge of 600 autoclave cycles, with a mean lifetime of more than 450 cycles, multiple times more than prescribed number of cycles. Instrument reprocessing is among the important aspects of infection control. While stringent procedures are followed in instrument reprocessing within the clinic in assuring patient safety, regular use of sterilization process indicators and periodic biological validation of the sterilizer's performance is necessary. Chemical indicators for use in Autoclaves provide information on whether the particular cycle's parameters were achieved but do not provide at what specific point in time or temperature the failure occurred. Data loggers and associated reader software as the tested prototype in this evaluation (DS1922F), are designed to provide continuous information on time and temperature of the prescribed cycle. Data loggers provide immediate information on the process as opposed to Biological Indicators that take from days to a week in obtaining a confirmatory result. Further, many countries do not have the sterilization monitoring service infrastructure to meet the demands of the end users. In the absence of sterilization monitoring services, use of digital data loggers for each sterilization cycle is more pragmatic.

  18. 40 CFR 63.1325 - Batch process vents-performance test methods and procedures to determine compliance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... either total organic HAP or TOC. For purposes of this paragraph (c), the term “batch emission episode... ensure the measurement of total organic HAP or TOC (minus methane and ethane) concentrations in all batch... organic HAP or TOC, as appropriate. Alternatively, any other method or data that has been validated...

  19. Free Factories: Unified Infrastructure for Data Intensive Web Services

    PubMed Central

    Zaranek, Alexander Wait; Clegg, Tom; Vandewege, Ward; Church, George M.

    2010-01-01

    We introduce the Free Factory, a platform for deploying data-intensive web services using small clusters of commodity hardware and free software. Independently administered virtual machines called Freegols give application developers the flexibility of a general purpose web server, along with access to distributed batch processing, cache and storage services. Each cluster exploits idle RAM and disk space for cache, and reserves disks in each node for high bandwidth storage. The batch processing service uses a variation of the MapReduce model. Virtualization allows every CPU in the cluster to participate in batch jobs. Each 48-node cluster can achieve 4-8 gigabytes per second of disk I/O. Our intent is to use multiple clusters to process hundreds of simultaneous requests on multi-hundred terabyte data sets. Currently, our applications achieve 1 gigabyte per second of I/O with 123 disks by scheduling batch jobs on two clusters, one of which is located in a remote data center. PMID:20514356

  20. Efficacy of rabies immunoglobulins in an experimental post-exposure prophylaxis rodent model.

    PubMed

    Servat, Alexandre; Lutsch, Charles; Delore, Valentine; Lang, Jean; Veitch, Keith; Cliquet, Florence

    2003-12-12

    In a recently published Syrian hamster animal challenge study [Vaccine 19 (2001) 2273], a highly purified, heat-treated equine rabies immunoglobulin (pERIG HT, Favirab) did not elicit satisfactory protection. The efficacies of this batch, a second stage pERIG HT batch and reference RIG preparations (Imorab, Imogam Rage pasteurised, Berna antiserum) were compared in mice challenged with either Ariana canine field strain or CVS strain. Survival rates against Ariana challenge with the second pERIG HT batch were indistinguishable from those of other licensed preparations (83-90% survival), but the deficient batch did not provide satisfactory protection (53%). These data confirm the inadequate response to a first stage pERIG HT batch, but a current batch provides equivalent protection to that afforded by licensed HRIG and ERIG preparations.

  1. Production of nattokinase by batch and fed-batch culture of Bacillus subtilis.

    PubMed

    Cho, Young-Han; Song, Jae Yong; Kim, Kyung Mi; Kim, Mi Kyoung; Lee, In Young; Kim, Sang Bum; Kim, Hyeon Shup; Han, Nam Soo; Lee, Bong Hee; Kim, Beom Soo

    2010-09-30

    Nattokinase was produced by batch and fed-batch culture of Bacillus subtilis in flask and fermentor. Effect of supplementing complex media (peptone, yeast extract, or tryptone) was investigated on the production of nattokinase. In flask culture, the highest cell growth and nattokinase activity were obtained with 50 g/L of peptone supplementation. In this condition, nattokinase activity was 630 unit/ml at 12 h. In batch culture of B. subtilis in fermentor, the highest nattokinase activity of 3400 unit/ml was obtained at 10h with 50 g/L of peptone supplementation. From the batch kinetics data, it was shown that nattokinase production was growth-associated and culture should be harvested before stationary phase for maximum nattokinase production. In fed-batch culture of B. subtilis using pH-stat feeding strategy, cell growth (optical density monitored at 600 nm) increased to ca. 100 at 22 h, which was 2.5 times higher than that in batch culture. The highest nattokinase activity was 7100 unit/ml at 19 h, which was also 2.1 times higher than that in batch culture. Copyright 2010 Elsevier B.V. All rights reserved.

  2. Using surface-enhanced Raman spectroscopy and electrochemically driven melting to discriminate Yersinia pestis from Y. pseudotuberculosis based on single nucleotide polymorphisms within unpurified polymerase chain reaction amplicons.

    PubMed

    Papadopoulou, Evanthia; Goodchild, Sarah A; Cleary, David W; Weller, Simon A; Gale, Nittaya; Stubberfield, Michael R; Brown, Tom; Bartlett, Philip N

    2015-02-03

    The development of sensors for the detection of pathogen-specific DNA, including relevant species/strain level discrimination, is critical in molecular diagnostics with major impacts in areas such as bioterrorism and food safety. Herein, we use electrochemically driven denaturation assays monitored by surface-enhanced Raman spectroscopy (SERS) to target single nucleotide polymorphisms (SNPs) that distinguish DNA amplicons generated from Yersinia pestis, the causative agent of plague, from the closely related species Y. pseudotuberculosis. Two assays targeting SNPs within the groEL and metH genes of these two species have been successfully designed. Polymerase chain reaction (PCR) was used to produce Texas Red labeled single-stranded DNA (ssDNA) amplicons of 262 and 251 bases for the groEL and metH targets, respectively. These amplicons were used in an unpurified form to hybridize to immobilized probes then subjected to electrochemically driven melting. In all cases electrochemically driven melting was able to discriminate between fully homologous DNA and that containing SNPs. The metH assay was particularly challenging due to the presence of only a single base mismatch in the middle of the 251 base long PCR amplicon. However, manipulation of assay conditions (conducting the electrochemical experiments at 10 °C) resulted in greater discrimination between the complementary and mismatched DNA. Replicate data were collected and analyzed for each duplex on different days, using different batches of PCR product and different sphere segment void (SSV) substrates. Despite the variability introduced by these differences, the assays are shown to be reliable and robust providing a new platform for strain discrimination using unpurified PCR samples.

  3. Quantitative Accelerated Life Testing of MEMS Accelerometers.

    PubMed

    Bâzu, Marius; Gălăţeanu, Lucian; Ilian, Virgil Emil; Loicq, Jerome; Habraken, Serge; Collette, Jean-Paul

    2007-11-20

    Quantitative Accelerated Life Testing (QALT) is a solution for assessing thereliability of Micro Electro Mechanical Systems (MEMS). A procedure for QALT is shownin this paper and an attempt to assess the reliability level for a batch of MEMSaccelerometers is reported. The testing plan is application-driven and contains combinedtests: thermal (high temperature) and mechanical stress. Two variants of mechanical stressare used: vibration (at a fixed frequency) and tilting. Original equipment for testing at tiltingand high temperature is used. Tilting is appropriate as application-driven stress, because thetilt movement is a natural environment for devices used for automotive and aerospaceapplications. Also, tilting is used by MEMS accelerometers for anti-theft systems. The testresults demonstrated the excellent reliability of the studied devices, the failure rate in the"worst case" being smaller than 10 -7 h -1 .

  4. Hierarchical Bayesian models to assess between- and within-batch variability of pathogen contamination in food.

    PubMed

    Commeau, Natalie; Cornu, Marie; Albert, Isabelle; Denis, Jean-Baptiste; Parent, Eric

    2012-03-01

    Assessing within-batch and between-batch variability is of major interest for risk assessors and risk managers in the context of microbiological contamination of food. For example, the ratio between the within-batch variability and the between-batch variability has a large impact on the results of a sampling plan. Here, we designed hierarchical Bayesian models to represent such variability. Compatible priors were built mathematically to obtain sound model comparisons. A numeric criterion is proposed to assess the contamination structure comparing the ability of the models to replicate grouped data at the batch level using a posterior predictive loss approach. Models were applied to two case studies: contamination by Listeria monocytogenes of pork breast used to produce diced bacon and contamination by the same microorganism on cold smoked salmon at the end of the process. In the first case study, a contamination structure clearly exists and is located at the batch level, that is, between batches variability is relatively strong, whereas in the second a structure also exists but is less marked. © 2012 Society for Risk Analysis.

  5. Perfusion cell culture decreases process and product heterogeneity in a head-to-head comparison with fed-batch.

    PubMed

    Walther, Jason; Lu, Jiuyi; Hollenbach, Myles; Yu, Marcella; Hwang, Chris; McLarty, Jean; Brower, Kevin

    2018-05-30

    In this study, we compared the impacts of fed-batch and perfusion platforms on process and product attributes for IgG1- and IgG4-producing cell lines. A "plug-and-play" approach was applied to both platforms at bench scale, using commercially available basal and feed media, a standard feed strategy for fed-batch, and ATF filtration for perfusion. Product concentration in fed-batch was 2.5 times greater than perfusion, while average productivity in perfusion was 7.5 times greater than fed-batch. PCA revealed more variability in the cell environment and metabolism during the fed-batch run. LDH measurements showed that exposure of product to cell lysate was 7-10 times greater in fed-batch. Product analysis shows larger abundances of neutral species in perfusion, likely due to decreased bioreactor residence times and extracellular exposure. The IgG1 perfusion product also had higher purity and lower half-antibody. Glycosylation was similar across both culture modes. The first perfusion harvest slice for both product types showed different glycosylation than subsequent harvests, suggesting that product quality lags behind metabolism. In conclusion, process and product data indicate that intra-lot heterogeneity is decreased in perfusion cultures. Additional data and discussion is required to understand the developmental, clinical and commercial implications, and in what situations increased uniformity would be beneficial. This article is protected by copyright. All rights reserved.

  6. NHEXAS PHASE I ARIZONA STUDY--STANDARD OPERATING PROCEDURE FOR INSTRUCTIONS FOR THE ADDITION OF INDIVIDUAL CLEANED NON SCANNED DATA BATCHES TO MASTER DATABASES (UA-D-27.0)

    EPA Science Inventory

    The purpose of this SOP is to define the procedures involved in appending cleaned individual data batches to the master databases. This procedure applies to the Arizona NHEXAS project and the "Border" study. Keywords: data; appending.

    The National Human Exposure Assessment Sur...

  7. Collaborative study for the establishment of replacement batches of heparin low- molecular-mass for assay biological reference preparations.

    PubMed

    Terao, E; Daas, A; Rautmann, G; Buchheit, K-H

    2010-10-01

    A collaborative study was run by the European Directorate for the Quality of Medicines & HealthCare (EDQM) in the context of the Biological Standardisation Programme (BSP), under the aegis of the Council of Europe and the European Commission, to establish replacement batches for the dwindling stocks of the Heparin low-molecular-mass for assay European Pharmacopoeia Biological Reference Preparation (BRP). The replacement batches of BRP are intended to be used in the assays for anti-Xa and anti-IIa activities, as described in the European Pharmacopoeia (Ph. Eur.) monograph Heparins, low-molecular-mass (0828). Three freeze-dried candidate batches were calibrated against the current International Standard (IS) for Heparin, lowmolecular- weight (2nd IS, 01/608). For the purpose of the continuity check between subsequent BRP batches, the current Heparin low-molecular-mass for assay BRP (batch 5) was also included in the test panel. Thirteen official medicines control and manufacturers laboratories from European and non-European countries contributed data. A central statistical analysis of the datasets was performed at the EDQM. On the basis of the results, the 3 candidate materials were assigned a potency of 104 IU/vial for the anti-Xa activity and 31 IU/vial for the anti-IIa activity. Taken into account the preliminary stability data and the results of this collaborative study, the 3 batches of candidate BRP were adopted in June 2010 by the Commission of the Ph. Eur. as Heparin low-molecular-mass for assay BRP batches 6, 7 and 8.

  8. FASTSim: A Model to Estimate Vehicle Efficiency, Cost and Performance

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Brooker, A.; Gonder, J.; Wang, L.

    2015-05-04

    The Future Automotive Systems Technology Simulator (FASTSim) is a high-level advanced vehicle powertrain systems analysis tool supported by the U.S. Department of Energy’s Vehicle Technologies Office. FASTSim provides a quick and simple approach to compare powertrains and estimate the impact of technology improvements on light- and heavy-duty vehicle efficiency, performance, cost, and battery batches of real-world drive cycles. FASTSim’s calculation framework and balance among detail, accuracy, and speed enable it to simulate thousands of driven miles in minutes. The key components and vehicle outputs have been validated by comparing the model outputs to test data for many different vehicles tomore » provide confidence in the results. A graphical user interface makes FASTSim easy and efficient to use. FASTSim is freely available for download from the National Renewable Energy Laboratory’s website (see www.nrel.gov/fastsim).« less

  9. Direct infusion mass spectrometry metabolomics dataset: a benchmark for data processing and quality control

    PubMed Central

    Kirwan, Jennifer A; Weber, Ralf J M; Broadhurst, David I; Viant, Mark R

    2014-01-01

    Direct-infusion mass spectrometry (DIMS) metabolomics is an important approach for characterising molecular responses of organisms to disease, drugs and the environment. Increasingly large-scale metabolomics studies are being conducted, necessitating improvements in both bioanalytical and computational workflows to maintain data quality. This dataset represents a systematic evaluation of the reproducibility of a multi-batch DIMS metabolomics study of cardiac tissue extracts. It comprises of twenty biological samples (cow vs. sheep) that were analysed repeatedly, in 8 batches across 7 days, together with a concurrent set of quality control (QC) samples. Data are presented from each step of the workflow and are available in MetaboLights. The strength of the dataset is that intra- and inter-batch variation can be corrected using QC spectra and the quality of this correction assessed independently using the repeatedly-measured biological samples. Originally designed to test the efficacy of a batch-correction algorithm, it will enable others to evaluate novel data processing algorithms. Furthermore, this dataset serves as a benchmark for DIMS metabolomics, derived using best-practice workflows and rigorous quality assessment. PMID:25977770

  10. Active Job Monitoring in Pilots

    NASA Astrophysics Data System (ADS)

    Kuehn, Eileen; Fischer, Max; Giffels, Manuel; Jung, Christopher; Petzold, Andreas

    2015-12-01

    Recent developments in high energy physics (HEP) including multi-core jobs and multi-core pilots require data centres to gain a deep understanding of the system to monitor, design, and upgrade computing clusters. Networking is a critical component. Especially the increased usage of data federations, for example in diskless computing centres or as a fallback solution, relies on WAN connectivity and availability. The specific demands of different experiments and communities, but also the need for identification of misbehaving batch jobs, requires an active monitoring. Existing monitoring tools are not capable of measuring fine-grained information at batch job level. This complicates network-aware scheduling and optimisations. In addition, pilots add another layer of abstraction. They behave like batch systems themselves by managing and executing payloads of jobs internally. The number of real jobs being executed is unknown, as the original batch system has no access to internal information about the scheduling process inside the pilots. Therefore, the comparability of jobs and pilots for predicting run-time behaviour or network performance cannot be ensured. Hence, identifying the actual payload is important. At the GridKa Tier 1 centre a specific tool is in use that allows the monitoring of network traffic information at batch job level. This contribution presents the current monitoring approach and discusses recent efforts and importance to identify pilots and their substructures inside the batch system. It will also show how to determine monitoring data of specific jobs from identified pilots. Finally, the approach is evaluated.

  11. Defining level A IVIVC dissolution specifications based on individual in vitro dissolution profiles of a controlled release formulation.

    PubMed

    González-García, I; García-Arieta, A; Merino-Sanjuan, M; Mangas-Sanjuan, V; Bermejo, M

    2018-07-01

    Regulatory guidelines recommend that, when a level A IVIVC is established, dissolution specification should be established using averaged data and the maximum difference between AUC and C max between the reference and test formulations cannot be greater than 20%. However, averaging data assumes a loss of information and may reflect a bias in the results. The objective of the current work is to present a new approach to establish dissolution specifications using a new methodology (individual approach) instead of average data (classical approach). Different scenarios were established based on the relationship between in vitro-in vivo dissolution rate coefficient using a level A IVIVC of a controlled release formulation. Then, in order to compare this new approach with the classical one, six additional batches were simulated. For each batch, 1000 simulations of a dissolution assay were run. C max ratios between the reference formulation and each batch were calculated showing that the individual approach was more sensitive and able to detect differences between the reference and the batch formulation compared to the classical approach. Additionally, the new methodology displays wider dissolution specification limits than the classical approach, ensuring that any tablet from the new batch would generate in vivo profiles which its AUC or C max ratio will be out of the 0.8-1.25 range, taking into account the in vitro and in vivo variability of the new batches developed. Copyright © 2018 Elsevier B.V. All rights reserved.

  12. GIDEP Batching Tool

    NASA Technical Reports Server (NTRS)

    Fong, Danny; Odell,Dorice; Barry, Peter; Abrahamian, Tomik

    2008-01-01

    This software provides internal, automated search mechanics of GIDEP (Government- Industry Data Exchange Program) Alert data imported from the GIDEP government Web site. The batching tool allows the import of a single parts list in tab-delimited text format into the local JPL GIDEP database. Delimiters from every part number are removed. The original part numbers with delimiters are compared, as well as the newly generated list without the delimiters. The two lists run against the GIDEP imports, and output any matches. This feature only works with Netscape 2.0 or greater, or Internet Explorer 4.0 or greater. The user selects the browser button to choose a text file to import. When the submit button is pressed, this script will import alerts from the text file into the local JPL GIDEP database. This batch tool provides complete in-house control over exported material and data for automated batch match abilities. The batching tool has the ability to match capabilities of the parts list to tables, and yields results that aid further research and analysis. This provides more control over GIDEP information for metrics and reports information not provided by the government site. This software yields results quickly and gives more control over external data from the government site in order to generate other reports not available from the external source. There is enough space to store years of data. The program relates to risk identification and management with regard to projects and GIDEP alert information encompassing flight parts for space exploration.

  13. NDA BATCH 2002-02

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lawrence Livermore National Laboratory

    2009-12-09

    QC sample results (daily background checks, 20-gram and 100-gram SGS drum checks) were within acceptable criteria established by WIPP's Quality Assurance Objectives for TRU Waste Characterization. Replicate runs were performed on 5 drums with IDs LL85101099TRU, LL85801147TRU, LL85801109TRU, LL85300999TRU and LL85500979TRU. All replicate measurement results are identical at the 95% confidence level as established by WIPP criteria. Note that the batch covered 5 weeks of SGS measurements from 23-Jan-2002 through 22-Feb-2002. Data packet for SGS Batch 2002-02 generated using gamma spectroscopy with the Pu Facility SGS unit is technically reasonable. All QC samples are in compliance with established control limits.more » The batch data packet has been reviewed for correctness, completeness, consistency and compliance with WIPP's Quality Assurance Objectives and determined to be acceptable. An Expert Review was performed on the data packet between 28-Feb-02 and 09-Jul-02 to check for potential U-235, Np-237 and Am-241 interferences and address drum cases where specific scan segments showed Se gamma ray transmissions for the 136-keV gamma to be below 0.1 %. Two drums in the batch showed Pu-238 at a relative mass ratio more than 2% of all the Pu isotopes.« less

  14. CHANGES IN CHLOROPHYLL A FLOURESCENCE AND PIGMENT RATIOS DURING DIFFERENT GROWTH PHASES OF A UNICELLULAR MARINE CHAETOCEROS (BACILLAROPHYCEAE) IN BATCH CULTURE

    EPA Science Inventory

    Interpretations of chlorophyll a fluorescence data are based largely on application with green algae and higher plants. This study evaluated the interpretation of fluorescence data for a unicellular marine diatom. Chaetoceros sp. was grown in 4-liter batch cultures on a 16:8, L:D...

  15. Collaborative study for the calibration of the Ph. Eur. prekallikrein activator in albumin BRP batches 4, 5 and 6.

    PubMed

    Lackner, F; Daas, A; Terao, E

    2015-01-01

    An international collaborative study was organised by the European Directorate for the Quality of Medicines & HealthCare (EDQM, Council of Europe) to calibrate replacement batches for the current European Pharmacopoeia (Ph. Eur.) prekallikrein activator (PKA) in albumin biological reference preparation (BRP), whose stocks were dwindling. The study was run in the framework of the Biological Standardisation Programme (BSP) of the Council of Europe and the European Union (EU) Commission. Twenty three laboratories from official medicines control authorities and manufacturers in Europe and outside Europe took part in the study. Three candidate replacement batches were produced from the same material as the one used for the World Health Organization (WHO) 2(nd) International Standard (IS) for PKA in albumin (02/168) and the Ph. Eur. PKA in albumin BRP batches 1, 2 and 3. Participants were requested to evaluate the candidate batches against the current WHO IS using their routine assay method. The Ph. Eur. PKA in albumin BRP batch 3 (BRP3) was also included in the test panel to ensure the continuity of the consecutive BRP batches. The study confirmed the stability of the PKA content of the current BRP3. The candidate batches were found to be comparable. Previous data on the starting material support its high stability. Thermal stress study on the candidate batches confirmed the stability of their PKA activity. The Commission of the Ph. Eur. officially adopted in November 2013 the 3 candidate batches as Ph. Eur. PKA in albumin BRP batches 4, 5 and 6 with an assigned content of 38 IU/vial. The activity of the 3 new batches of Ph. Eur. PKA in albumin BRP will be regularly monitored.

  16. Development of a mathematical model for the growth associated Polyhydroxybutyrate fermentation by Azohydromonas australica and its use for the design of fed-batch cultivation strategies.

    PubMed

    Gahlawat, Geeta; Srivastava, Ashok K

    2013-06-01

    In the present investigation, batch cultivation of Azohydromonas australica DSM 1124 was carried out in a bioreactor for growth associated PHB production. The observed batch PHB production kinetics data was then used for the development of a mathematical model which adequately described the substrate limitation and inhibition during the cultivation. The statistical validity test demonstrated that the proposed mathematical model predictions were significant at 99% confidence level. The model was thereafter extrapolated to fed-batch to identify various nutrients feeding regimes during the bioreactor cultivation to improve the PHB accumulation. The distinct capability of the mathematical model to predict highly dynamic fed-batch cultivation strategies was demonstrated by experimental implementation of two fed-batch cultivation strategies. A significantly high PHB concentration of 22.65 g/L & an overall PHB content of 76% was achieved during constant feed rate fed-batch cultivation which is the highest PHB content reported so far using A. australica. Copyright © 2013 Elsevier Ltd. All rights reserved.

  17. U.S.-MEXICO BORDER PROGRAM ARIZONA BORDER STUDY--STANDARD OPERATING PROCEDURE FOR INSTRUCTIONS FOR THE ADDITION OF INDIVIDUAL CLEANED NON SCANNED DATA BATCHES TO MASTER DATABASES (UA-D-27.0)

    EPA Science Inventory

    The purpose of this SOP is to define the procedures involved in appending cleaned individual data batches to the master databases. This procedure applies to the Arizona NHEXAS project and the Border study. Keywords: data; database.

    The U.S.-Mexico Border Program is sponsored b...

  18. Segmentation, dynamic storage, and variable loading on CDC equipment

    NASA Technical Reports Server (NTRS)

    Tiffany, S. H.

    1980-01-01

    Techniques for varying the segmented load structure of a program and for varying the dynamic storage allocation, depending upon whether a batch type or interactive type run is desired, are explained and demonstrated. All changes are based on a single data input to the program. The techniques involve: code within the program to suppress scratch pad input/output (I/O) for a batch run or translate the in-core data storage area from blank common to the end-of-code+1 address of a particular segment for an interactive run; automatic editing of the segload directives prior to loading, based upon data input to the program, to vary the structure of the load for interactive and batch runs; and automatic editing of the load map to determine the initial addresses for in core data storage for an interactive run.

  19. Tackling the widespread and critical impact of batch effects in high-throughput data.

    PubMed

    Leek, Jeffrey T; Scharpf, Robert B; Bravo, Héctor Corrada; Simcha, David; Langmead, Benjamin; Johnson, W Evan; Geman, Donald; Baggerly, Keith; Irizarry, Rafael A

    2010-10-01

    High-throughput technologies are widely used, for example to assay genetic variants, gene and protein expression, and epigenetic modifications. One often overlooked complication with such studies is batch effects, which occur because measurements are affected by laboratory conditions, reagent lots and personnel differences. This becomes a major problem when batch effects are correlated with an outcome of interest and lead to incorrect conclusions. Using both published studies and our own analyses, we argue that batch effects (as well as other technical and biological artefacts) are widespread and critical to address. We review experimental and computational approaches for doing so.

  20. Bioprocessing Data for the Production of Marine Enzymes

    PubMed Central

    Sarkar, Sreyashi; Pramanik, Arnab; Mitra, Anindita; Mukherjee, Joydeep

    2010-01-01

    This review is a synopsis of different bioprocess engineering approaches adopted for the production of marine enzymes. Three major modes of operation: batch, fed-batch and continuous have been used for production of enzymes (such as protease, chitinase, agarase, peroxidase) mainly from marine bacteria and fungi on a laboratory bioreactor and pilot plant scales. Submerged, immobilized and solid-state processes in batch mode were widely employed. The fed-batch process was also applied in several bioprocesses. Continuous processes with suspended cells as well as with immobilized cells have been used. Investigations in shake flasks were conducted with the prospect of large-scale processing in reactors. PMID:20479981

  1. Application of the International Water Association activated sludge models to describe aerobic sludge digestion.

    PubMed

    Ghorbani, M; Eskicioglu, C

    2011-12-01

    Batch and semi-continuous flow aerobic digesters were used to stabilize thickened waste-activated sludge at different initial conditions and mean solids retention times. Under dynamic conditions, total suspended solids, volatile suspended solids (VSS) and total and particulate chemical oxygen demand (COD and PCOD) were monitored in the batch reactors and effluent from the semi-continuous flow reactors. Activated Sludge Model (ASM) no. 1 and ASM no. 3 were applied to measured data (calibration data set) to evaluate the consistency and performances of models at different flow regimes for digester COD and VSS modelling. The results indicated that both ASM1 and ASM3 predicted digester COD, VSS and PCOD concentrations well (R2, Ra2 > or = 0.93). Parameter estimation concluded that compared to ASM1, ASM3 parameters were more consistent across different batch and semi-continuous flow runs with different operating conditions. Model validation on a data set independent from the calibration data successfully predicted digester COD (R2 = 0.88) and VSS (R2 = 0.94) concentrations by ASM3, while ASM1 overestimated both reactor COD (R2 = 0.74) and VSS concentrations (R2 = 0.79) after 15 days of aerobic batch digestion.

  2. Direct analysis in real time mass spectrometry, a process analytical technology tool for real-time process monitoring in botanical drug manufacturing.

    PubMed

    Wang, Lu; Zeng, Shanshan; Chen, Teng; Qu, Haibin

    2014-03-01

    A promising process analytical technology (PAT) tool has been introduced for batch processes monitoring. Direct analysis in real time mass spectrometry (DART-MS), a means of rapid fingerprint analysis, was applied to a percolation process with multi-constituent substances for an anti-cancer botanical preparation. Fifteen batches were carried out, including ten normal operations and five abnormal batches with artificial variations. The obtained multivariate data were analyzed by a multi-way partial least squares (MPLS) model. Control trajectories were derived from eight normal batches, and the qualification was tested by R(2) and Q(2). Accuracy and diagnosis capability of the batch model were then validated by the remaining batches. Assisted with high performance liquid chromatography (HPLC) determination, process faults were explained by corresponding variable contributions. Furthermore, a batch level model was developed to compare and assess the model performance. The present study has demonstrated that DART-MS is very promising in process monitoring in botanical manufacturing. Compared with general PAT tools, DART-MS offers a particular account on effective compositions and can be potentially used to improve batch quality and process consistency of samples in complex matrices. Copyright © 2014 Elsevier B.V. All rights reserved.

  3. DOE Office of Scientific and Technical Information (OSTI.GOV)

    Akcakaya, Murat; Nehorai, Arye; Sen, Satyabrata

    Most existing radar algorithms are developed under the assumption that the environment (clutter) is stationary. However, in practice, the characteristics of the clutter can vary enormously depending on the radar-operational scenarios. If unaccounted for, these nonstationary variabilities may drastically hinder the radar performance. Therefore, to overcome such shortcomings, we develop a data-driven method for target detection in nonstationary environments. In this method, the radar dynamically detects changes in the environment and adapts to these changes by learning the new statistical characteristics of the environment and by intelligibly updating its statistical detection algorithm. Specifically, we employ drift detection algorithms to detectmore » changes in the environment; incremental learning, particularly learning under concept drift algorithms, to learn the new statistical characteristics of the environment from the new radar data that become available in batches over a period of time. The newly learned environment characteristics are then integrated in the detection algorithm. Furthermore, we use Monte Carlo simulations to demonstrate that the developed method provides a significant improvement in the detection performance compared with detection techniques that are not aware of the environmental changes.« less

  4. "Batch" kinetics in flow: online IR analysis and continuous control.

    PubMed

    Moore, Jason S; Jensen, Klavs F

    2014-01-07

    Currently, kinetic data is either collected under steady-state conditions in flow or by generating time-series data in batch. Batch experiments are generally considered to be more suitable for the generation of kinetic data because of the ability to collect data from many time points in a single experiment. Now, a method that rapidly generates time-series reaction data from flow reactors by continuously manipulating the flow rate and reaction temperature has been developed. This approach makes use of inline IR analysis and an automated microreactor system, which allowed for rapid and tight control of the operating conditions. The conversion/residence time profiles at several temperatures were used to fit parameters to a kinetic model. This method requires significantly less time and a smaller amount of starting material compared to one-at-a-time flow experiments, and thus allows for the rapid generation of kinetic data. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  5. PHYTO-REMOVAL OF TRINITROTOLUENE FROM WATER WITH BATCH KINETIC STUDIES

    EPA Science Inventory

    A series of batch reactor studies were conducted to obtain kinetic data for optimizing phyto-treatment of water contaminated with trinitrotoluene (TNT). A plant screening study indicated that stonewort and parrotfeather were the most effective among the plants tested; parrotfeath...

  6. Quantitative Accelerated Life Testing of MEMS Accelerometers

    PubMed Central

    Bâzu, Marius; Gălăţeanu, Lucian; Ilian, Virgil Emil; Loicq, Jerome; Habraken, Serge; Collette, Jean-Paul

    2007-01-01

    Quantitative Accelerated Life Testing (QALT) is a solution for assessing the reliability of Micro Electro Mechanical Systems (MEMS). A procedure for QALT is shown in this paper and an attempt to assess the reliability level for a batch of MEMS accelerometers is reported. The testing plan is application-driven and contains combined tests: thermal (high temperature) and mechanical stress. Two variants of mechanical stress are used: vibration (at a fixed frequency) and tilting. Original equipment for testing at tilting and high temperature is used. Tilting is appropriate as application-driven stress, because the tilt movement is a natural environment for devices used for automotive and aerospace applications. Also, tilting is used by MEMS accelerometers for anti-theft systems. The test results demonstrated the excellent reliability of the studied devices, the failure rate in the “worst case” being smaller than 10-7h-1. PMID:28903265

  7. A scalable architecture for online anomaly detection of WLCG batch jobs

    NASA Astrophysics Data System (ADS)

    Kuehn, E.; Fischer, M.; Giffels, M.; Jung, C.; Petzold, A.

    2016-10-01

    For data centres it is increasingly important to monitor the network usage, and learn from network usage patterns. Especially configuration issues or misbehaving batch jobs preventing a smooth operation need to be detected as early as possible. At the GridKa data and computing centre we therefore operate a tool BPNetMon for monitoring traffic data and characteristics of WLCG batch jobs and pilots locally on different worker nodes. On the one hand local information itself are not sufficient to detect anomalies for several reasons, e.g. the underlying job distribution on a single worker node might change or there might be a local misconfiguration. On the other hand a centralised anomaly detection approach does not scale regarding network communication as well as computational costs. We therefore propose a scalable architecture based on concepts of a super-peer network.

  8. Network acceleration techniques

    NASA Technical Reports Server (NTRS)

    Crowley, Patricia (Inventor); Maccabe, Arthur Barney (Inventor); Awrach, James Michael (Inventor)

    2012-01-01

    Splintered offloading techniques with receive batch processing are described for network acceleration. Such techniques offload specific functionality to a NIC while maintaining the bulk of the protocol processing in the host operating system ("OS"). The resulting protocol implementation allows the application to bypass the protocol processing of the received data. Such can be accomplished this by moving data from the NIC directly to the application through direct memory access ("DMA") and batch processing the receive headers in the host OS when the host OS is interrupted to perform other work. Batch processing receive headers allows the data path to be separated from the control path. Unlike operating system bypass, however, the operating system still fully manages the network resource and has relevant feedback about traffic and flows. Embodiments of the present disclosure can therefore address the challenges of networks with extreme bandwidth delay products (BWDP).

  9. Batch Tests To Determine Activity Distribution and Kinetic Parameters for Acetate Utilization in Expanded-Bed Anaerobic Reactors

    PubMed Central

    Fox, Peter; Suidan, Makram T.

    1990-01-01

    Batch tests to measure maximum acetate utilization rates were used to determine the distribution of acetate utilizers in expanded-bed sand and expanded-bed granular activated carbon (GAC) reactors. The reactors were fed a mixture of acetate and 3-ethylphenol, and they contained the same predominant aceticlastic methanogen, Methanothrix sp. Batch tests were performed both on the entire reactor contents and with media removed from the reactors. Results indicated that activity was evenly distributed within the GAC reactors, whereas in the sand reactor a sludge blanket on top of the sand bed contained approximately 50% of the activity. The Monod half-velocity constant (Ks) for the acetate-utilizing methanogens in two expanded-bed GAC reactors was searched for by combining steady-state results with batch test data. All parameters necessary to develop a model with Monod kinetics were experimentally determined except for Ks. However, Ks was a function of the effluent 3-ethylphenol concentration, and batch test results demonstrated that maximum acetate utilization rates were not a function of the effluent 3-ethylphenol concentration. Addition of a competitive inhibition term into the Monod expression predicted the dependence of Ks on the effluent 3-ethylphenol concentration. A two-parameter search determined a Ks of 8.99 mg of acetate per liter and a Ki of 2.41 mg of 3-ethylphenol per liter. Model predictions were in agreement with experimental observations for all effluent 3-ethylphenol concentrations. Batch tests measured the activity for a specific substrate and determined the distribution of activity in the reactor. The use of steady-state data in conjunction with batch test results reduced the number of unknown kinetic parameters and thereby reduced the uncertainty in the results and the assumptions made. PMID:16348175

  10. Batch tests to determine activity distribution and kinetic parameters for acetate utilization in expanded-bed anaerobic reactors.

    PubMed

    Fox, P; Suidan, M T

    1990-04-01

    Batch tests to measure maximum acetate utilization rates were used to determine the distribution of acetate utilizers in expanded-bed sand and expanded-bed granular activated carbon (GAC) reactors. The reactors were fed a mixture of acetate and 3-ethylphenol, and they contained the same predominant aceticlastic methanogen, Methanothrix sp. Batch tests were performed both on the entire reactor contents and with media removed from the reactors. Results indicated that activity was evenly distributed within the GAC reactors, whereas in the sand reactor a sludge blanket on top of the sand bed contained approximately 50% of the activity. The Monod half-velocity constant (K(s)) for the acetate-utilizing methanogens in two expanded-bed GAC reactors was searched for by combining steady-state results with batch test data. All parameters necessary to develop a model with Monod kinetics were experimentally determined except for K(s). However, K(s) was a function of the effluent 3-ethylphenol concentration, and batch test results demonstrated that maximum acetate utilization rates were not a function of the effluent 3-ethylphenol concentration. Addition of a competitive inhibition term into the Monod expression predicted the dependence of K(s) on the effluent 3-ethylphenol concentration. A two-parameter search determined a K(s) of 8.99 mg of acetate per liter and a K(i) of 2.41 mg of 3-ethylphenol per liter. Model predictions were in agreement with experimental observations for all effluent 3-ethylphenol concentrations. Batch tests measured the activity for a specific substrate and determined the distribution of activity in the reactor. The use of steady-state data in conjunction with batch test results reduced the number of unknown kinetic parameters and thereby reduced the uncertainty in the results and the assumptions made.

  11. Characterising and correcting batch variation in an automated direct infusion mass spectrometry (DIMS) metabolomics workflow.

    PubMed

    Kirwan, J A; Broadhurst, D I; Davidson, R L; Viant, M R

    2013-06-01

    Direct infusion mass spectrometry (DIMS)-based untargeted metabolomics measures many hundreds of metabolites in a single experiment. While every effort is made to reduce within-experiment analytical variation in untargeted metabolomics, unavoidable sources of measurement error are introduced. This is particularly true for large-scale multi-batch experiments, necessitating the development of robust workflows that minimise batch-to-batch variation. Here, we conducted a purpose-designed, eight-batch DIMS metabolomics study using nanoelectrospray (nESI) Fourier transform ion cyclotron resonance mass spectrometric analyses of mammalian heart extracts. First, we characterised the intrinsic analytical variation of this approach to determine whether our existing workflows are fit for purpose when applied to a multi-batch investigation. Batch-to-batch variation was readily observed across the 7-day experiment, both in terms of its absolute measurement using quality control (QC) and biological replicate samples, as well as its adverse impact on our ability to discover significant metabolic information within the data. Subsequently, we developed and implemented a computational workflow that includes total-ion-current filtering, QC-robust spline batch correction and spectral cleaning, and provide conclusive evidence that this workflow reduces analytical variation and increases the proportion of significant peaks. We report an overall analytical precision of 15.9%, measured as the median relative standard deviation (RSD) for the technical replicates of the biological samples, across eight batches and 7 days of measurements. When compared against the FDA guidelines for biomarker studies, which specify an RSD of <20% as an acceptable level of precision, we conclude that our new workflows are fit for purpose for large-scale, high-throughput nESI DIMS metabolomics studies.

  12. Effect of skill laboratory training on academic performance of medical students.

    PubMed

    Khan, Muhammad Alamgir; Shabbir, Faizania; Qamar, Khadija; Rajput, Tausif Ahmed

    2017-05-01

    To observe the effect of skill lab training on academic performance of final year medical students in terms of marks obtained in long case, short case, objective structured clinical examination and viva. The cross-sectional comparative study was conducted at Army Medical College, Rawalpindi from February to April 2015. Two batches of final year MBBS were recruited for the study. Batch 1 received conventional training, and Batch 2 received skill lab training. The performance of students was assessed by comparing the marks obtained in long case, short case, objective structured clinical examination and viva. Data was analysed using SPSS 23. Of the 335 subjects, 168(50.1%) were male and 167(49.9%) were female students with a mean age of 21.79±1.02 years. Batch 1 had 151(45%) students and Batch 2 had 184(55%). Batch 2 got significantly higher marks in long case, short case and objective structured clinical examination (p<0.05 each). Viva result was not found to be related to training (p>0.05). Acquisition of clinical skills significantly improved when medial students were trained in skill laboratories.

  13. A GPU-Accelerated Approach for Feature Tracking in Time-Varying Imagery Datasets.

    PubMed

    Peng, Chao; Sahani, Sandip; Rushing, John

    2017-10-01

    We propose a novel parallel connected component labeling (CCL) algorithm along with efficient out-of-core data management to detect and track feature regions of large time-varying imagery datasets. Our approach contributes to the big data field with parallel algorithms tailored for GPU architectures. We remove the data dependency between frames and achieve pixel-level parallelism. Due to the large size, the entire dataset cannot fit into cached memory. Frames have to be streamed through the memory hierarchy (disk to CPU main memory and then to GPU memory), partitioned, and processed as batches, where each batch is small enough to fit into the GPU. To reconnect the feature regions that are separated due to data partitioning, we present a novel batch merging algorithm to extract the region connection information across multiple batches in a parallel fashion. The information is organized in a memory-efficient structure and supports fast indexing on the GPU. Our experiment uses a commodity workstation equipped with a single GPU. The results show that our approach can efficiently process a weather dataset composed of terabytes of time-varying radar images. The advantages of our approach are demonstrated by comparing to the performance of an efficient CPU cluster implementation which is being used by the weather scientists.

  14. Ethanol production improvement driven by genome-scale metabolic modeling and sensitivity analysis in Scheffersomyces stipitis

    PubMed Central

    2017-01-01

    The yeast Scheffersomyces stipitis naturally produces ethanol from xylose, however reaching high ethanol yields is strongly dependent on aeration conditions. It has been reported that changes in the availability of NAD(H/+) cofactors can improve fermentation in some microorganisms. In this work genome-scale metabolic modeling and phenotypic phase plane analysis were used to characterize metabolic response on a range of uptake rates. Sensitivity analysis was used to assess the effect of ARC on ethanol production indicating that modifying ARC by inhibiting the respiratory chain ethanol production can be improved. It was shown experimentally in batch culture using Rotenone as an inhibitor of the mitochondrial NADH dehydrogenase complex I (CINADH), increasing ethanol yield by 18%. Furthermore, trajectories for uptakes rates, specific productivity and specific growth rate were determined by modeling the batch culture, to calculate ARC associated to the addition of CINADH inhibitor. Results showed that the increment in ethanol production via respiratory inhibition is due to excess in ARC, which generates an increase in ethanol production. Thus ethanol production improvement could be predicted by a change in ARC. PMID:28658270

  15. Ethanol production improvement driven by genome-scale metabolic modeling and sensitivity analysis in Scheffersomyces stipitis.

    PubMed

    Acevedo, Alejandro; Conejeros, Raúl; Aroca, Germán

    2017-01-01

    The yeast Scheffersomyces stipitis naturally produces ethanol from xylose, however reaching high ethanol yields is strongly dependent on aeration conditions. It has been reported that changes in the availability of NAD(H/+) cofactors can improve fermentation in some microorganisms. In this work genome-scale metabolic modeling and phenotypic phase plane analysis were used to characterize metabolic response on a range of uptake rates. Sensitivity analysis was used to assess the effect of ARC on ethanol production indicating that modifying ARC by inhibiting the respiratory chain ethanol production can be improved. It was shown experimentally in batch culture using Rotenone as an inhibitor of the mitochondrial NADH dehydrogenase complex I (CINADH), increasing ethanol yield by 18%. Furthermore, trajectories for uptakes rates, specific productivity and specific growth rate were determined by modeling the batch culture, to calculate ARC associated to the addition of CINADH inhibitor. Results showed that the increment in ethanol production via respiratory inhibition is due to excess in ARC, which generates an increase in ethanol production. Thus ethanol production improvement could be predicted by a change in ARC.

  16. Stochastic Averaging for Constrained Optimization With Application to Online Resource Allocation

    NASA Astrophysics Data System (ADS)

    Chen, Tianyi; Mokhtari, Aryan; Wang, Xin; Ribeiro, Alejandro; Giannakis, Georgios B.

    2017-06-01

    Existing approaches to resource allocation for nowadays stochastic networks are challenged to meet fast convergence and tolerable delay requirements. The present paper leverages online learning advances to facilitate stochastic resource allocation tasks. By recognizing the central role of Lagrange multipliers, the underlying constrained optimization problem is formulated as a machine learning task involving both training and operational modes, with the goal of learning the sought multipliers in a fast and efficient manner. To this end, an order-optimal offline learning approach is developed first for batch training, and it is then generalized to the online setting with a procedure termed learn-and-adapt. The novel resource allocation protocol permeates benefits of stochastic approximation and statistical learning to obtain low-complexity online updates with learning errors close to the statistical accuracy limits, while still preserving adaptation performance, which in the stochastic network optimization context guarantees queue stability. Analysis and simulated tests demonstrate that the proposed data-driven approach improves the delay and convergence performance of existing resource allocation schemes.

  17. Mineralogical characterization of selected shales in support of nuclear waste repository studies: Progress report, October 1987--September 1988

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Lee, S. Y.; Hyder, L. K.; Baxter, P. M.

    1989-07-01

    One objective of the Sedimentary Rock Program at the Oak Ridge National Laboratory has been to examine end-member shales to develop a data base that will aid in evaluations if shales are ever considered as a repository host rock. Five end-member shales were selected for comprehensive characterization: the Chattanooga Shale from Fentress County, Tennessee; the Pierre Shale from Gregory County, South Dakota; the Green River Formation from Garfield County, Colorado; and the Nolichucky Shale and Pumpkin Valley Shale from Roane County, Tennessee. Detailed micromorphological and mineralogical characterizations of the shales were completed by Lee et al. (1987) in ORNL/TM-10567. Thismore » report is a supplemental characterization study that was necessary because second batches of the shale samples were needed for additional studies. Selected physical, chemical, and mineralogical properties were determined for the second batches; and their properties were compared with the results from the first batches. Physical characterization indicated that the second-batch and first-batch samples had a noticeable difference in apparent-size distributions but had similar primary-particle-size distributions. There were some differences in chemical composition between the batches, but these differences were not considered important in comparison with the differences among the end-member shales. The results of x-ray diffraction analyses showed that the second batches had mineralogical compositions very similar to the first batches. 9 refs., 9 figs., 4 tabs.« less

  18. Acceptance Test Data for Candidate AGR-5/6/7 TRISO Particle Batches BWXT Coater Batches 93165 93172 Defective IPyC Fraction and Pyrocarbon Anisotropy

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Helmreich, Grant W.; Hunn, John D.; Skitt, Darren J.

    2017-03-01

    Coated particle fuel batches J52O-16-93165, 93166, 93168, 93169, 93170, and 93172 were produced by Babcock and Wilcox Technologies (BWXT) for possible selection as fuel for the Advanced Gas Reactor Fuel Development and Qualification (AGR) Program’s AGR-5/6/7 irradiation test in the Idaho National Laboratory (INL) Advanced Test Reactor (ATR). Some of these batches may alternately be used as demonstration coated particle fuel for other experiments. Each batch was coated in a 150-mm-diameter production-scale fluidized-bed chemical vapor deposition (CVD) furnace. Tristructural isotropic (TRISO) coatings were deposited on 425-μm-nominal-diameter spherical kernels from BWXT lot J52R-16-69317 containing a mixture of 15.5%-enriched uranium carbide andmore » uranium oxide (UCO). The TRISO coatings consisted of four consecutive CVD layers: a ~50% dense carbon buffer layer with 100-μm-nominal thickness, a dense inner pyrolytic carbon (IPyC) layer with 40-μm-nominal thickness, a silicon carbide (SiC) layer with 35-μm-nominal thickness, and a dense outer pyrolytic carbon (OPyC) layer with 40-μmnominal thickness. The TRISO-coated particle batches were sieved to upgrade the particles by removing over-sized and under-sized material, and the upgraded batches were designated by appending the letter A to the end of the batch number (e.g., 93165A).« less

  19. Collaborative study for the calibration of replacement batches for the heparin low-molecular-mass for assay biological reference preparation.

    PubMed

    Terao, E; Daas, A

    2016-01-01

    The European Pharmacopoeia (Ph. Eur.) prescribes the control of the activity of low molecular mass heparins by assays for anti-Xa and anti-IIa activities (monograph 0828), using a reference standard calibrated in International Units (IU). An international collaborative study coded BSP133 was launched in the framework of the Biological Standardisation Programme (BSP) run under the aegis of the Council of Europe and the European Commission to calibrate replacement batches for the dwindling stocks of the Heparin low-molecular-mass for assay Biological Reference Preparation (BRP) batch 8. Thirteen official medicines control and manufacturers laboratories from European and non-European countries took part in this study to calibrate two freeze-dried candidate batches against the 3rd International Standard (IS) for heparin, low molecular weight (11/176; 3rd IS). The Heparin low-molecular-mass for assay BRP (batch 8) was also included in the test panel to check the continuity between subsequent BRP batches. Taking into account the stability data, the results of this collaborative study and on the basis of the central statistical analysis performed at the European Directorate for the Quality of Medicines & HealthCare (EDQM), the 2 candidate batches were officially adopted by the Commission of the European Pharmacopoeia as Heparin low-molecular-mass for assay BRP batches 9 and 10 with assigned anti-Xa activities of 102 and 100 IU/vial and anti-IIa activities of 34 and 33 IU/vial respectively.

  20. Computional algorithm for lifetime exposure to antimicrobials in pigs using register data-The LEA algorithm.

    PubMed

    Birkegård, Anna Camilla; Andersen, Vibe Dalhoff; Halasa, Tariq; Jensen, Vibeke Frøkjær; Toft, Nils; Vigre, Håkan

    2017-10-01

    Accurate and detailed data on antimicrobial exposure in pig production are essential when studying the association between antimicrobial exposure and antimicrobial resistance. Due to difficulties in obtaining primary data on antimicrobial exposure in a large number of farms, there is a need for a robust and valid method to estimate the exposure using register data. An approach that estimates the antimicrobial exposure in every rearing period during the lifetime of a pig using register data was developed into a computational algorithm. In this approach data from national registers on antimicrobial purchases, movements of pigs and farm demographics registered at farm level are used. The algorithm traces batches of pigs retrospectively from slaughter to the farm(s) that housed the pigs during their finisher, weaner, and piglet period. Subsequently, the algorithm estimates the antimicrobial exposure as the number of Animal Defined Daily Doses for treatment of one kg pig in each of the rearing periods. Thus, the antimicrobial purchase data at farm level are translated into antimicrobial exposure estimates at batch level. A batch of pigs is defined here as pigs sent to slaughter at the same day from the same farm. In this study we present, validate, and optimise a computational algorithm that calculate the lifetime exposure of antimicrobials for slaughter pigs. The algorithm was evaluated by comparing the computed estimates to data on antimicrobial usage from farm records in 15 farm units. We found a good positive correlation between the two estimates. The algorithm was run for Danish slaughter pigs sent to slaughter in January to March 2015 from farms with more than 200 finishers to estimate the proportion of farms that it was applicable for. In the final process, the algorithm was successfully run for batches of pigs originating from 3026 farms with finisher units (77% of the initial population). This number can be increased if more accurate register data can be obtained. The algorithm provides a systematic and repeatable approach to estimating the antimicrobial exposure throughout the rearing period, independent of rearing site for finisher batches, as a lifetime exposure measurement. Copyright © 2017 Elsevier B.V. All rights reserved.

  1. [Evaluation of pipetting systems. III. Micropipette precision in a routine task].

    PubMed

    Salas, R; Loría, A; Rocha, C

    1995-01-01

    To establish a norm of the precision achievable with a micropipette in an IRMA assay under routine conditions. A micropipette (Gilson) adjusted to dispense 100 microL was used by a single analyst with experience in its use. In each assay, ten aliquots of radioactive antiprolactin were pipetted in clean tubes (PRE-batch tubes), followed by pipetting of the tubes being processed in the assay, and at the end, a second pipetting of 10 aliquots in clean tubes (POST-batch tubes). The study includes the data of 15 consecutive batches during a seven month period with an overall mean of 283 tubes per batch. The PRE- and POST-tubes were read in a gamma counter (Crystal plus). The mean, SD and CV for PRE, POST and global (PRE+POST) tubes were calculated for each batch. The global CV of the 15 batches ranged from 1.6 to 6.9%, mean of 3.1%. We found no evidence of increased imprecision due to fatigue of the analyst, but surprisingly, we observed that in nine of the 15 batches there was a significant difference in the means of the PRE-tubes vs the POST-tubes (t test) without differences in precision. Thus, part of the global variability is due to what we have called pseudoimprecision (i.e. an increase in CV due to differences in means). In addition, the POST-tubes had higher values in the first 7 batches but the opposite occurred in the last 8 batches (table 2). This shift in the sign of the PRE-POST differences suggests the presence of opposite factors operating in time, i.e. one or more factors increased the volume of pipetting after using the pipette more than 150 times (batches 1-7) whereas other/others decreased it (batches 8-15). 1. Our first approximation to a norm of micropipetting precision in batches of 200-300 tubes was a CV of 3.1%. 2. This norm was influenced by a problem of pseudoimprecision detected ex-post-facto. 3. Our findings justify continuation studies to detect the pseudoimprecision and evaluate its causes prospectively.

  2. A short term quality control tool for biodegradable microspheres.

    PubMed

    D'Souza, Susan; Faraj, Jabar A; Dorati, Rossella; DeLuca, Patrick P

    2014-06-01

    Accelerated in vitro release testing methodology has been developed as an indicator of product performance to be used as a discriminatory quality control (QC) technique for the release of clinical and commercial batches of biodegradable microspheres. While product performance of biodegradable microspheres can be verified by in vivo and/or in vitro experiments, such evaluation can be particularly challenging because of slow polymer degradation, resulting in extended study times, labor, and expense. Three batches of Leuprolide poly(lactic-co-glycolic acid) (PLGA) microspheres having varying morphology (process variants having different particle size and specific surface area) were manufactured by the solvent extraction/evaporation technique. Tests involving in vitro release, polymer degradation and hydration of the microspheres were performed on the three batches at 55°C. In vitro peptide release at 55°C was analyzed using a previously derived modification of the Weibull function termed the modified Weibull equation (MWE). Experimental observations and data analysis confirm excellent reproducibility studies within and between batches of the microsphere formulations demonstrating the predictability of the accelerated experiments at 55°C. The accelerated test method was also successfully able to distinguish the in vitro product performance between the three batches having varying morphology (process variants), indicating that it is a suitable QC tool to discriminate product or process variants in clinical or commercial batches of microspheres. Additionally, data analysis utilized the MWE to further quantify the differences obtained from the accelerated in vitro product performance test between process variants, thereby enhancing the discriminatory power of the accelerated methodology at 55°C.

  3. Soft sensor modeling based on variable partition ensemble method for nonlinear batch processes

    NASA Astrophysics Data System (ADS)

    Wang, Li; Chen, Xiangguang; Yang, Kai; Jin, Huaiping

    2017-01-01

    Batch processes are always characterized by nonlinear and system uncertain properties, therefore, the conventional single model may be ill-suited. A local learning strategy soft sensor based on variable partition ensemble method is developed for the quality prediction of nonlinear and non-Gaussian batch processes. A set of input variable sets are obtained by bootstrapping and PMI criterion. Then, multiple local GPR models are developed based on each local input variable set. When a new test data is coming, the posterior probability of each best performance local model is estimated based on Bayesian inference and used to combine these local GPR models to get the final prediction result. The proposed soft sensor is demonstrated by applying to an industrial fed-batch chlortetracycline fermentation process.

  4. Characterizing optical polishing pitch

    NASA Astrophysics Data System (ADS)

    Varshneya, Rupal; DeGroote, Jessica E.; Gregg, Leslie L.; Jacobs, Stephen D.

    2003-05-01

    Characterization data for five experimental optical polishing pitch products were compared to those for corresponding standard commercial optical polishing pitches. The experimental pitches were tested for three physical properties: hardness, viscosity at 90°C, and softening point. A Shore A Durometer test was used to measure hardness. Viscosity data were collected using a Stony Brook Scientific falling needle viscometer. Softening point was determined using the ASTM D3104-97 method. Results demonstrate that the softest and the hardest batches of the experimental grades of optical pitch are comparable to the industry-accepted standards, while the other grades of pitch are not. The experimental methodology followed in this research may allow opticians to rapidly compare different brands of pitch to help identify batch-to-batch differences and control pitch quality before use.

  5. Characterizing optical polishing pitch

    NASA Astrophysics Data System (ADS)

    Varshneya, Rupal

    2003-05-01

    Characterization data for five experimental optical polishing pitch products were compared to those for corresponding standard commercial optical polishing pitches. The experimental pitches were tested for three physical properties: hardness, viscosity at 90°C, and softening point. A Shore A Durometerl test was used to measure hardness. Viscosity data were collected using a Stony Brook Scientific' falling needle viscometer. Softening point was determined using the ASTM D3104-97 method. Results demonstrate that the softest and the hardest batches of the experimental grades of optical pitch are comparable to the industry-accepted standards, while the other grades of pitch are not. The experimental methodology followed in this research may allow opticians to rapidly compare different brands of pitch to help identify batch- to- batch differences and control pitch quality before use.

  6. ARTS: automated randomization of multiple traits for study design.

    PubMed

    Maienschein-Cline, Mark; Lei, Zhengdeng; Gardeux, Vincent; Abbasi, Taimur; Machado, Roberto F; Gordeuk, Victor; Desai, Ankit A; Saraf, Santosh; Bahroos, Neil; Lussier, Yves

    2014-06-01

    Collecting data from large studies on high-throughput platforms, such as microarray or next-generation sequencing, typically requires processing samples in batches. There are often systematic but unpredictable biases from batch-to-batch, so proper randomization of biologically relevant traits across batches is crucial for distinguishing true biological differences from experimental artifacts. When a large number of traits are biologically relevant, as is common for clinical studies of patients with varying sex, age, genotype and medical background, proper randomization can be extremely difficult to prepare by hand, especially because traits may affect biological inferences, such as differential expression, in a combinatorial manner. Here we present ARTS (automated randomization of multiple traits for study design), which aids researchers in study design by automatically optimizing batch assignment for any number of samples, any number of traits and any batch size. ARTS is implemented in Perl and is available at github.com/mmaiensc/ARTS. ARTS is also available in the Galaxy Tool Shed, and can be used at the Galaxy installation hosted by the UIC Center for Research Informatics (CRI) at galaxy.cri.uic.edu. © The Author 2014. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  7. The influence of pH adjustment on kinetics parameters in tapioca wastewater treatment using aerobic sequencing batch reactor system

    NASA Astrophysics Data System (ADS)

    Mulyani, Happy; Budianto, Gregorius Prima Indra; Margono, Kaavessina, Mujtahid

    2018-02-01

    The present investigation deals with the aerobic sequencing batch reactor system of tapioca wastewater treatment with varying pH influent conditions. This project was carried out to evaluate the effect of pH on kinetics parameters of system. It was done by operating aerobic sequencing batch reactor system during 8 hours in many tapioca wastewater conditions (pH 4.91, pH 7, pH 8). The Chemical Oxygen Demand (COD) and Mixed Liquor Volatile Suspended Solids (MLVSS) of the aerobic sequencing batch reactor system effluent at steady state condition were determined at interval time of two hours to generate data for substrate inhibition kinetics parameters. Values of the kinetics constants were determined using Monod and Andrews models. There was no inhibition constant (Ki) detected in all process variation of aerobic sequencing batch reactor system for tapioca wastewater treatment in this study. Furthermore, pH 8 was selected as the preferred aerobic sequencing batch reactor system condition in those ranging pH investigated due to its achievement of values of kinetics parameters such µmax = 0.010457/hour and Ks = 255.0664 mg/L COD.

  8. Bacteriophage PRD1 batch experiments to study attachment, detachment and inactivation processes

    NASA Astrophysics Data System (ADS)

    Sadeghi, Gholamreza; Schijven, Jack F.; Behrends, Thilo; Hassanizadeh, S. Majid; van Genuchten, Martinus Th.

    2013-09-01

    Knowledge of virus removal in subsurface environments is pivotal for assessing the risk of viral contamination of water resources and developing appropriate protection measures. Columns packed with sand are frequently used to quantify attachment, detachment and inactivation rates of viruses. Since column transport experiments are very laborious, a common alternative is to perform batch experiments where usually one or two measurements are done assuming equilibrium is reached. It is also possible to perform kinetic batch experiments. In that case, however, it is necessary to monitor changes in the concentration with time. This means that kinetic batch experiments will be almost as laborious as column experiments. Moreover, attachment and detachment rate coefficients derived from batch experiments may differ from those determined using column experiments. The aim of this study was to determine the utility of kinetic batch experiments and investigate the effects of different designs of the batch experiments on estimated attachment, detachment and inactivation rate coefficients. The experiments involved various combinations of container size, sand-water ratio, and mixing method (i.e., rolling or tumbling by pivoting the tubes around their horizontal or vertical axes, respectively). Batch experiments were conducted with clean quartz sand, water at pH 7 and ionic strength of 20 mM, and using the bacteriophage PRD1 as a model virus. Values of attachment, detachment and inactivation rate coefficients were found by fitting an analytical solution of the kinetic model equations to the data. Attachment rate coefficients were found to be systematically higher under tumbling than under rolling conditions because of better mixing and more efficient contact of phages with the surfaces of the sand grains. In both mixing methods, more sand in the container yielded higher attachment rate coefficients. A linear increase in the detachment rate coefficient was observed with increased solid-water ratio using tumbling method. Given the differences in the attachment rate coefficients, and assuming the same sticking efficiencies since chemical conditions of the batch and column experiments were the same, our results show that collision efficiencies of batch experiments are not the same as those of column experiments. Upscaling of the attachment rate from batch to column experiments hence requires proper understanding of the mixing conditions. Because batch experiments, in which the kinetics are monitored, are as laborious as column experiments, there seems to be no major advantage in performing batch instead of column experiments.

  9. How Many Batches Are Needed for Process Validation under the New FDA Guidance?

    PubMed

    Yang, Harry

    2013-01-01

    The newly updated FDA Guidance for Industry on Process Validation: General Principles and Practices ushers in a life cycle approach to process validation. While the guidance no longer considers the use of traditional three-batch validation appropriate, it does not prescribe the number of validation batches for a prospective validation protocol, nor does it provide specific methods to determine it. This potentially could leave manufacturers in a quandary. In this paper, I develop a Bayesian method to address the issue. By combining process knowledge gained from Stage 1 Process Design (PD) with expected outcomes of Stage 2 Process Performance Qualification (PPQ), the number of validation batches for PPQ is determined to provide a high level of assurance that the process will consistently produce future batches meeting quality standards. Several examples based on simulated data are presented to illustrate the use of the Bayesian method in helping manufacturers make risk-based decisions for Stage 2 PPQ, and they highlight the advantages of the method over traditional Frequentist approaches. The discussions in the paper lend support for a life cycle and risk-based approach to process validation recommended in the new FDA guidance. The newly updated FDA Guidance for Industry on Process Validation: General Principles and Practices ushers in a life cycle approach to process validation. While the guidance no longer considers the use of traditional three-batch validation appropriate, it does not prescribe the number of validation batches for a prospective validation protocol, nor does it provide specific methods to determine it. This potentially could leave manufacturers in a quandary. In this paper, I develop a Bayesian method to address the issue. By combining process knowledge gained from Stage 1 Process Design (PD) with expected outcomes of Stage 2 Process Performance Qualification (PPQ), the number of validation batches for PPQ is determined to provide a high level of assurance that the process will consistently produce future batches meeting quality standards. Several examples based on simulated data are presented to illustrate the use of the Bayesian method in helping manufacturers make risk-based decisions for Stage 2 PPQ, and THEY highlight the advantages of the method over traditional Frequentist approaches. The discussions in the paper lend support for a life cycle and risk-based approach to process validation recommended in the new FDA guidance.

  10. Experimental evaluation of a recursive model identification technique for type 1 diabetes.

    PubMed

    Finan, Daniel A; Doyle, Francis J; Palerm, Cesar C; Bevier, Wendy C; Zisser, Howard C; Jovanovic, Lois; Seborg, Dale E

    2009-09-01

    A model-based controller for an artificial beta cell requires an accurate model of the glucose-insulin dynamics in type 1 diabetes subjects. To ensure the robustness of the controller for changing conditions (e.g., changes in insulin sensitivity due to illnesses, changes in exercise habits, or changes in stress levels), the model should be able to adapt to the new conditions by means of a recursive parameter estimation technique. Such an adaptive strategy will ensure that the most accurate model is used for the current conditions, and thus the most accurate model predictions are used in model-based control calculations. In a retrospective analysis, empirical dynamic autoregressive exogenous input (ARX) models were identified from glucose-insulin data for nine type 1 diabetes subjects in ambulatory conditions. Data sets consisted of continuous (5-minute) glucose concentration measurements obtained from a continuous glucose monitor, basal insulin infusion rates and times and amounts of insulin boluses obtained from the subjects' insulin pumps, and subject-reported estimates of the times and carbohydrate content of meals. Two identification techniques were investigated: nonrecursive, or batch methods, and recursive methods. Batch models were identified from a set of training data, whereas recursively identified models were updated at each sampling instant. Both types of models were used to make predictions of new test data. For the purpose of comparison, model predictions were compared to zero-order hold (ZOH) predictions, which were made by simply holding the current glucose value constant for p steps into the future, where p is the prediction horizon. Thus, the ZOH predictions are model free and provide a base case for the prediction metrics used to quantify the accuracy of the model predictions. In theory, recursive identification techniques are needed only when there are changing conditions in the subject that require model adaptation. Thus, the identification and validation techniques were performed with both "normal" data and data collected during conditions of reduced insulin sensitivity. The latter were achieved by having the subjects self-administer a medication, prednisone, for 3 consecutive days. The recursive models were allowed to adapt to this condition of reduced insulin sensitivity, while the batch models were only identified from normal data. Data from nine type 1 diabetes subjects in ambulatory conditions were analyzed; six of these subjects also participated in the prednisone portion of the study. For normal test data, the batch ARX models produced 30-, 45-, and 60-minute-ahead predictions that had average root mean square error (RMSE) values of 26, 34, and 40 mg/dl, respectively. For test data characterized by reduced insulin sensitivity, the batch ARX models produced 30-, 60-, and 90-minute-ahead predictions with average RMSE values of 27, 46, and 59 mg/dl, respectively; the recursive ARX models demonstrated similar performance with corresponding values of 27, 45, and 61 mg/dl, respectively. The identified ARX models (batch and recursive) produced more accurate predictions than the model-free ZOH predictions, but only marginally. For test data characterized by reduced insulin sensitivity, RMSE values for the predictions of the batch ARX models were 9, 5, and 5% more accurate than the ZOH predictions for prediction horizons of 30, 60, and 90 minutes, respectively. In terms of RMSE values, the 30-, 60-, and 90-minute predictions of the recursive models were more accurate than the ZOH predictions, by 10, 5, and 2%, respectively. In this experimental study, the recursively identified ARX models resulted in predictions of test data that were similar, but not superior, to the batch models. Even for the test data characteristic of reduced insulin sensitivity, the batch and recursive models demonstrated similar prediction accuracy. The predictions of the identified ARX models were only marginally more accurate than the model-free ZOH predictions. Given the simplicity of the ARX models and the computational ease with which they are identified, however, even modest improvements may justify the use of these models in a model-based controller for an artificial beta cell. 2009 Diabetes Technology Society.

  11. Fate and transport with material response characterization of green sorption media for copper removal via desorption process.

    PubMed

    Chang, Ni-Bin; Houmann, Cameron; Lin, Kuen-Song; Wanielista, Martin

    2016-07-01

    Multiple adsorption and desorption cycles are required to achieve the reliable operation of copper removal and recovery. A green sorption media mixture composed of recycled tire chunk, expanded clay aggregate, and coconut coir was evaluated in this study for its desorptive characteristics as a companion study of the corresponding adsorption process in an earlier publication. We conducted a screening of potential desorbing agents, batch desorption equilibrium and kinetic studies, and batch tests through 3 adsorption/desorption cycles. The desorbing agent screening revealed that hydrochloric acid has good potential for copper desorption. Equilibrium data fit the Freundlich isotherm, whereas kinetic data had high correlation with the Lagergren pseudo second-order model and revealed a rapid desorption reaction. Batch equilibrium data over 3 adsorption/desorption cycles showed that the coconut coir and media mixture were the most resilient, demonstrating they could be used through 3 or more adsorption/desorption cycles. FE-SEM imaging, XRD, and EDS analyses supported the batch adsorption and desorption results showing significant surface sorption of CuO species in the media mixture and coconut coir, followed by partial desorption using 0.1 M HCl as a desorbing agent. Copyright © 2016 Elsevier Ltd. All rights reserved.

  12. Potential of biogenic hydrogen production for hydrogen driven remediation strategies in marine environments.

    PubMed

    Hosseinkhani, Baharak; Hennebel, Tom; Boon, Nico

    2014-09-25

    Fermentative production of bio-hydrogen (bio-H2) from organic residues has emerged as a promising alternative for providing the required electron source for hydrogen driven remediation strategies. Unlike the widely used production of H2 by bacteria in fresh water systems, few reports are available regarding the generation of biogenic H2 and optimisation processes in marine systems. The present research aims to optimise the capability of an indigenous marine bacterium for the production of bio-H2 in marine environments and subsequently develop this process for hydrogen driven remediation strategies. Fermentative conversion of organics in marine media to H2 using a marine isolate, Pseudoalteromonas sp. BH11, was determined. A Taguchi design of experimental methodology was employed to evaluate the optimal nutritional composition in batch tests to improve bio-H2 yields. Further optimisation experiments showed that alginate-immobilised bacterial cells were able to produce bio-H2 at the same rate as suspended cells over a period of several weeks. Finally, bio-H2 was used as electron donor to successfully dehalogenate trichloroethylene (TCE) using biogenic palladium nanoparticles as a catalyst. Fermentative production of bio-H2 can be a promising technique for concomitant generation of an electron source for hydrogen driven remediation strategies and treatment of organic residue in marine ecosystems. Copyright © 2014 Elsevier B.V. All rights reserved.

  13. Surfactant-enhanced remediation of a trichloroethene-contaminated aquifer. 1. Transport of triton X-100

    USGS Publications Warehouse

    Smith, J.A.; Sahoo, D.; Mclellan, H.M.; Imbrigiotta, T.E.

    1997-01-01

    Transport of a nonionic surfactant (Triton X-100) at aqueous concentrations less than 400 mg/L through a trichloroethene-contaminated sand-and-gravel aquifer at Picatinny Arsenal, NJ, has been studied through a series of laboratory and field experiments. In the laboratory, batch and column experiments were conducted to quantify the rate and amount of Triton X-100 sorption to the aquifer sediments. In the field, a 400 mg/L aqueous Triton X-100 solution was injected into the aquifer at a rate of 26.5 L/min for a 35-d period. The transport of Triton X-100 was monitored by sampling and analysis of groundwater at six locations surrounding the injection well. Equilibrium batch sorption experiments showed that Triton X-100 sorbs strongly and nonlinearly to the field soil with the sharpest inflection point of the isotherm occurring at an equilibrium aqueous Triton X-100 concentration close to critical micelle concentration. Batch, soil column, and field experimental data were analyzed with zero-, one-, and two- dimensional (respectively) transient solute transport models with either equilibrium or rate-limited sorption. These analyses reveal that Triton X- 100 sorption to the aquifer solids is slow relative to advective and dispersive transport and that an equilibrium sorption model cannot simulate accurately the observed soil column and field data. Comparison of kinetic sorption parameters from batch, column, and field transport data indicate that both physical heterogeneities and Triton X-100 mass transfer between water and soil contribute to the kinetic transport effects.Transport of a nonionic surfactant (Triton X-100) at aqueous concentrations less than 400 mg/L through a trichloroethene-contaminated sand-and-gravel aquifer was studied. Equilibrium batch sorption experiments showed that Triton X-100 sorbs strongly and nonlinearly to the field soil with the sharpest inflection point of the isotherm occurring at an equilibrium aqueous Triton X-100 concentration close to critical micelle concentration. Batch, soil column, and field experimental data were analyzed with zero-, one-, and two-dimensional transient solute transport models with either equilibrium or rate-limited sorption. These analyses revealed that Triton X-100 sorption to the aquifer solids was slow relative to advective and dispersive transport.

  14. HPLC-MS Examination of Impurities in Pentaerythritol Tetranitrate

    NASA Astrophysics Data System (ADS)

    Brown, Geoffrey W.; Giambra, Anna M.

    2014-04-01

    Pentaerythritol tetranitrate (PETN) has trace homolog impurities that can be detected by high-performance liquid chromatography-mass spectrometry. Consideration of observed impurity masses and candidate structures based on known pentaerythritol impurities allows identification of 22 compounds in the data. These are all consistent with either fully nitrated homologs or derivatives substituted with methyl, methoxy, or hydroxyl groups in place of a nitric ester. Examining relative impurity concentrations in three starting batches of PETN and six subsequently processed batches shows that it is possible to use relative concentration profiles as a fingerprint to differentiate batches and follow them through recrystallization steps.

  15. Modeling of the pyruvate production with Escherichia coli: comparison of mechanistic and neural networks-based models.

    PubMed

    Zelić, B; Bolf, N; Vasić-Racki, D

    2006-06-01

    Three different models: the unstructured mechanistic black-box model, the input-output neural network-based model and the externally recurrent neural network model were used to describe the pyruvate production process from glucose and acetate using the genetically modified Escherichia coli YYC202 ldhA::Kan strain. The experimental data were used from the recently described batch and fed-batch experiments [ Zelić B, Study of the process development for Escherichia coli-based pyruvate production. PhD Thesis, University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, Croatia, July 2003. (In English); Zelić et al. Bioproc Biosyst Eng 26:249-258 (2004); Zelić et al. Eng Life Sci 3:299-305 (2003); Zelić et al Biotechnol Bioeng 85:638-646 (2004)]. The neural networks were built out of the experimental data obtained in the fed-batch pyruvate production experiments with the constant glucose feed rate. The model validation was performed using the experimental results obtained from the batch and fed-batch pyruvate production experiments with the constant acetate feed rate. Dynamics of the substrate and product concentration changes was estimated using two neural network-based models for biomass and pyruvate. It was shown that neural networks could be used for the modeling of complex microbial fermentation processes, even in conditions in which mechanistic unstructured models cannot be applied.

  16. The development of an industrial-scale fed-batch fermentation simulation.

    PubMed

    Goldrick, Stephen; Ştefan, Andrei; Lovett, David; Montague, Gary; Lennox, Barry

    2015-01-10

    This paper describes a simulation of an industrial-scale fed-batch fermentation that can be used as a benchmark in process systems analysis and control studies. The simulation was developed using a mechanistic model and validated using historical data collected from an industrial-scale penicillin fermentation process. Each batch was carried out in a 100,000 L bioreactor that used an industrial strain of Penicillium chrysogenum. The manipulated variables recorded during each batch were used as inputs to the simulator and the predicted outputs were then compared with the on-line and off-line measurements recorded in the real process. The simulator adapted a previously published structured model to describe the penicillin fermentation and extended it to include the main environmental effects of dissolved oxygen, viscosity, temperature, pH and dissolved carbon dioxide. In addition the effects of nitrogen and phenylacetic acid concentrations on the biomass and penicillin production rates were also included. The simulated model predictions of all the on-line and off-line process measurements, including the off-gas analysis, were in good agreement with the batch records. The simulator and industrial process data are available to download at www.industrialpenicillinsimulation.com and can be used to evaluate, study and improve on the current control strategy implemented on this facility. Crown Copyright © 2014. Published by Elsevier B.V. All rights reserved.

  17. Rigorous buoyancy driven bubble mixing for centrifugal microfluidics.

    PubMed

    Burger, S; Schulz, M; von Stetten, F; Zengerle, R; Paust, N

    2016-01-21

    We present batch-mode mixing for centrifugal microfluidics operated at fixed rotational frequency. Gas is generated by the disk integrated decomposition of hydrogen peroxide (H2O2) to liquid water (H2O) and gaseous oxygen (O2) and inserted into a mixing chamber. There, bubbles are formed that ascent through the liquid in the artificial gravity field and lead to drag flow. Additionaly, strong buoyancy causes deformation and rupture of the gas bubbles and induces strong mixing flows in the liquids. Buoyancy driven bubble mixing is quantitatively compared to shake mode mixing, mixing by reciprocation and vortex mixing. To determine mixing efficiencies in a meaningful way, the different mixers are employed for mixing of a lysis reagent and human whole blood. Subsequently, DNA is extracted from the lysate and the amount of DNA recovered is taken as a measure for mixing efficiency. Relative to standard vortex mixing, DNA extraction based on buoyancy driven bubble mixing resulted in yields of 92 ± 8% (100 s mixing time) and 100 ± 8% (600 s) at 130g centrifugal acceleration. Shake mode mixing yields 96 ± 11% and is thus equal to buoyancy driven bubble mixing. An advantage of buoyancy driven bubble mixing is that it can be operated at fixed rotational frequency, however. The additional costs of implementing buoyancy driven bubble mixing are low since both the activation liquid and the catalyst are very low cost and no external means are required in the processing device. Furthermore, buoyancy driven bubble mixing can easily be integrated in a monolithic manner and is compatible to scalable manufacturing technologies such as injection moulding or thermoforming. We consider buoyancy driven bubble mixing an excellent alternative to shake mode mixing, in particular if the processing device is not capable of providing fast changes of rotational frequency or if the low average rotational frequency is challenging for the other integrated fluidic operations.

  18. AUTO-MUTE 2.0: A Portable Framework with Enhanced Capabilities for Predicting Protein Functional Consequences upon Mutation.

    PubMed

    Masso, Majid; Vaisman, Iosif I

    2014-01-01

    The AUTO-MUTE 2.0 stand-alone software package includes a collection of programs for predicting functional changes to proteins upon single residue substitutions, developed by combining structure-based features with trained statistical learning models. Three of the predictors evaluate changes to protein stability upon mutation, each complementing a distinct experimental approach. Two additional classifiers are available, one for predicting activity changes due to residue replacements and the other for determining the disease potential of mutations associated with nonsynonymous single nucleotide polymorphisms (nsSNPs) in human proteins. These five command-line driven tools, as well as all the supporting programs, complement those that run our AUTO-MUTE web-based server. Nevertheless, all the codes have been rewritten and substantially altered for the new portable software, and they incorporate several new features based on user feedback. Included among these upgrades is the ability to perform three highly requested tasks: to run "big data" batch jobs; to generate predictions using modified protein data bank (PDB) structures, and unpublished personal models prepared using standard PDB file formatting; and to utilize NMR structure files that contain multiple models.

  19. Radar Unix: a complete package for GPR data processing

    NASA Astrophysics Data System (ADS)

    Grandjean, Gilles; Durand, Herve

    1999-03-01

    A complete package for ground penetrating radar data interpretation including data processing, forward modeling and a case history database consultation is presented. Running on an Unix operating system, its architecture consists of a graphical user interface generating batch files transmitted to a library of processing routines. This design allows a better software maintenance and the possibility for the user to run processing or modeling batch files by itself and differed in time. A case history data base is available and consists of an hypertext document which can be consulted by using a standard HTML browser. All the software specifications are presented through a realistic example.

  20. CDC/1000: a Control Data Corporation remote batch terminal emulator for Hewlett-Packard minicomputers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Berg, D.E.

    1981-02-01

    The Control Data Corporation Type 200 User Terminal utilizes a unique communications protocol to provide users with batch mode remote terminal access to Control Data computers. CDC/1000 is a software subsystem that implements this protocol on Hewlett-Packard minicomputers running the Real Time Executive III, IV, or IVB operating systems. This report provides brief descriptions of the various software modules comprising CDC/1000, and contains detailed instructions for integrating CDC/1000 into the Hewlett Packard operating system and for operating UTERM, the user interface program for CDC/1000. 6 figures.

  1. Pulse-Flow Microencapsulation System

    NASA Technical Reports Server (NTRS)

    Morrison, Dennis R.

    2006-01-01

    The pulse-flow microencapsulation system (PFMS) is an automated system that continuously produces a stream of liquid-filled microcapsules for delivery of therapeutic agents to target tissues. Prior microencapsulation systems have relied on batch processes that involve transfer of batches between different apparatuses for different stages of production followed by sampling for acquisition of quality-control data, including measurements of size. In contrast, the PFMS is a single, microprocessor-controlled system that performs all processing steps, including acquisition of quality-control data. The quality-control data can be used as real-time feedback to ensure the production of large quantities of uniform microcapsules.

  2. Future Supply Chains Enabled by Continuous Processing—Opportunities and Challenges. May 20–21, 2014 Continuous Manufacturing Symposium

    PubMed Central

    Srai, Jagjit Singh; Badman, Clive; Krumme, Markus; Futran, Mauricio; Johnston, Craig

    2015-01-01

    This paper examines the opportunities and challenges facing the pharmaceutical industry in moving to a primarily “continuous processing”-based supply chain. The current predominantly “large batch” and centralized manufacturing system designed for the “blockbuster” drug has driven a slow-paced, inventory heavy operating model that is increasingly regarded as inflexible and unsustainable. Indeed, new markets and the rapidly evolving technology landscape will drive more product variety, shorter product life-cycles, and smaller drug volumes, which will exacerbate an already unsustainable economic model. Future supply chains will be required to enhance affordability and availability for patients and healthcare providers alike despite the increased product complexity. In this more challenging supply scenario, we examine the potential for a more pull driven, near real-time demand-based supply chain, utilizing continuous processing where appropriate as a key element of a more “flow-through” operating model. In this discussion paper on future supply chain models underpinned by developments in the continuous manufacture of pharmaceuticals, we have set out; The significant opportunities to moving to a supply chain flow-through operating model, with substantial opportunities in inventory reduction, lead-time to patient, and radically different product assurance/stability regimes. Scenarios for decentralized production models producing a greater variety of products with enhanced volume flexibility. Production, supply, and value chain footprints that are radically different from today's monolithic and centralized batch manufacturing operations. Clinical trial and drug product development cost savings that support more rapid scale-up and market entry models with early involvement of SC designers within New Product Development. The major supply chain and industrial transformational challenges that need to be addressed. The paper recognizes that although current batch operational performance in pharma is far from optimal and not necessarily an appropriate end-state benchmark for batch technology, the adoption of continuous supply chain operating models underpinned by continuous production processing, as full or hybrid solutions in selected product supply chains, can support industry transformations to deliver right-first-time quality at substantially lower inventory profiles. © 2015 The Authors. Journal of Pharmaceutical Sciences published by Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 104:840–849, 2015 PMID:25631279

  3. Bayesian assurance and sample size determination in the process validation life-cycle.

    PubMed

    Faya, Paul; Seaman, John W; Stamey, James D

    2017-01-01

    Validation of pharmaceutical manufacturing processes is a regulatory requirement and plays a key role in the assurance of drug quality, safety, and efficacy. The FDA guidance on process validation recommends a life-cycle approach which involves process design, qualification, and verification. The European Medicines Agency makes similar recommendations. The main purpose of process validation is to establish scientific evidence that a process is capable of consistently delivering a quality product. A major challenge faced by manufacturers is the determination of the number of batches to be used for the qualification stage. In this article, we present a Bayesian assurance and sample size determination approach where prior process knowledge and data are used to determine the number of batches. An example is presented in which potency uniformity data is evaluated using a process capability metric. By using the posterior predictive distribution, we simulate qualification data and make a decision on the number of batches required for a desired level of assurance.

  4. Quantitative imaging reveals heterogeneous growth dynamics and treatment-dependent residual tumor distributions in a three-dimensional ovarian cancer model

    NASA Astrophysics Data System (ADS)

    Celli, Jonathan P.; Rizvi, Imran; Evans, Conor L.; Abu-Yousif, Adnan O.; Hasan, Tayyaba

    2010-09-01

    Three-dimensional tumor models have emerged as valuable in vitro research tools, though the power of such systems as quantitative reporters of tumor growth and treatment response has not been adequately explored. We introduce an approach combining a 3-D model of disseminated ovarian cancer with high-throughput processing of image data for quantification of growth characteristics and cytotoxic response. We developed custom MATLAB routines to analyze longitudinally acquired dark-field microscopy images containing thousands of 3-D nodules. These data reveal a reproducible bimodal log-normal size distribution. Growth behavior is driven by migration and assembly, causing an exponential decay in spatial density concomitant with increasing mean size. At day 10, cultures are treated with either carboplatin or photodynamic therapy (PDT). We quantify size-dependent cytotoxic response for each treatment on a nodule by nodule basis using automated segmentation combined with ratiometric batch-processing of calcein and ethidium bromide fluorescence intensity data (indicating live and dead cells, respectively). Both treatments reduce viability, though carboplatin leaves micronodules largely structurally intact with a size distribution similar to untreated cultures. In contrast, PDT treatment disrupts micronodular structure, causing punctate regions of toxicity, shifting the distribution toward smaller sizes, and potentially increasing vulnerability to subsequent chemotherapeutic treatment.

  5. The European Partnership for Alternative Approaches to Animal Testing (EPAA): promoting alternative methods in Europe and beyond.

    PubMed

    Cozigou, Gwenole; Crozier, Jonathan; Hendriksen, Coenraad; Manou, Irene; Ramirez-Hernandez, Tzutzuy; Weissenhorn, Renate

    2015-03-01

    Here in we introduce the European Partnership for Alternative Approaches to Animal Testing (EPAA) and its activities, which are focused on international cooperation toward alternative methods. The EPAA is one of the leading organizations in Europe for the promotion of alternative approaches to animal testing. Its innovative public-private partnership structure enables a consensus-driven dialogue across 7 industry sectors to facilitate interaction between regulators and regulated stakeholders. Through a brief description of EPAA's activities and organizational structure, we first articulate the value of this collaboration; we then focus on 2 key projects driven by EPAA. The first project aims to address research gaps on stem cells for safety testing, whereas the second project strives for an approach toward demonstration of consistency in vaccine batch release testing. We highlight the growing need for harmonization of international acceptance and implementation of alternative approaches and for increased international collaboration to foster progress on nonanimal alternatives.

  6. A computer program for the generation of logic networks from task chart data

    NASA Technical Reports Server (NTRS)

    Herbert, H. E.

    1980-01-01

    The Network Generation Program (NETGEN), which creates logic networks from task chart data is presented. NETGEN is written in CDC FORTRAN IV (Extended) and runs in a batch mode on the CDC 6000 and CYBER 170 series computers. Data is input via a two-card format and contains information regarding the specific tasks in a project. From this data, NETGEN constructs a logic network of related activities with each activity having unique predecessor and successor nodes, activity duration, descriptions, etc. NETGEN then prepares this data on two files that can be used in the Project Planning Analysis and Reporting System Batch Network Scheduling program and the EZPERT graphics program.

  7. Comparison of batch and column tests for the elution of artificial turf system components.

    PubMed

    Krüger, O; Kalbe, U; Berger, W; Nordhauβ, K; Christoph, G; Walzel, H-P

    2012-12-18

    Synthetic athletic tracks and turf areas for outdoor sporting grounds may release contaminants due to the chemical composition of some components. A primary example is that of zinc from reused scrap tires (main constituent, styrene butadiene rubber, SBR), which might be harmful to the environment. Thus, methods for the risk assessment of those materials are required. Laboratory leaching methods like batch and column tests are widely used to examine the soil-groundwater pathway. We tested several components for artificial sporting grounds with batch tests at a liquid to solid (LS) ratio of 2 L/kg and column tests with an LS up to 26.5 L/kg. We found a higher zinc release in the batch test eluates for all granules, ranging from 15% higher to 687% higher versus data from column tests for SBR granules. Accompanying parameters, especially the very high turbidity of one ethylene propylene diene monomer rubber (EPDM) or thermoplastic elastomer (TPE) eluates, reflect the stronger mechanical stress of batch testing. This indicates that batch test procedures might not be suitable for the risk assessment of synthetic sporting ground components. Column tests, on the other hand, represent field conditions more closely and allow for determination of time-dependent contaminants release.

  8. Quality evaluation of Hypericum ascyron extract by two-dimensional high-performance liquid chromatography coupled with the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method.

    PubMed

    Li, Xiu-Mei; Luo, Xue-Gang; Zhang, Chao-Zheng; Wang, Nan; Zhang, Tong-Cun

    2015-02-01

    In this paper, a heart-cutting two-dimensional high-performance liquid chromatography coupled with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was established for controlling the quality of different batches of Hypericum ascyron extract for the first time. In comparison with the common one-dimensional fingerprint, the second-dimensional fingerprint compiled additional spectral data and was hence more informative. The quality of H. ascyron extract was further evaluated by similarity measures and the same results were achieved, the correlation coefficients of the similarity of ten batches of H. ascyron extract were ＞0.99. Furthermore, we also evaluated the quality of the ten batches of H. ascyron extract by antibacterial activity. The result demonstrated that the quality of the ten batches of H. ascyron extract was not significantly different by MTT. Finally, we demonstrated that the second-dimensional fingerprint coupled with the MTT method was a more powerful tool to characterize the quality of samples of batch to batch. Therefore the proposed method could be used to comprehensively conduct the quality control of traditional Chinese medicines. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  9. Extracting surface diffusion coefficients from batch adsorption measurement data: application of the classic Langmuir kinetics model.

    PubMed

    Chu, Khim Hoong

    2017-11-09

    Surface diffusion coefficients may be estimated by fitting solutions of a diffusion model to batch kinetic data. For non-linear systems, a numerical solution of the diffusion model's governing equations is generally required. We report here the application of the classic Langmuir kinetics model to extract surface diffusion coefficients from batch kinetic data. The use of the Langmuir kinetics model in lieu of the conventional surface diffusion model allows derivation of an analytical expression. The parameter estimation procedure requires determining the Langmuir rate coefficient from which the pertinent surface diffusion coefficient is calculated. Surface diffusion coefficients within the 10 -9 to 10 -6  cm 2 /s range obtained by fitting the Langmuir kinetics model to experimental kinetic data taken from the literature are found to be consistent with the corresponding values obtained from the traditional surface diffusion model. The virtue of this simplified parameter estimation method is that it reduces the computational complexity as the analytical expression involves only an algebraic equation in closed form which is easily evaluated by spreadsheet computation.

  10. Seven-Year Clinical Surveillance Program Demonstrates Consistent MARD Accuracy Performance of a Blood Glucose Test Strip.

    PubMed

    Setford, Steven; Grady, Mike; Mackintosh, Stephen; Donald, Robert; Levy, Brian

    2018-05-01

    MARD (mean absolute relative difference) is increasingly used to describe performance of glucose monitoring systems, providing a single-value quantitative measure of accuracy and allowing comparisons between different monitoring systems. This study reports MARDs for the OneTouch Verio® glucose meter clinical data set of 80 258 data points (671 individual batches) gathered as part of a 7.5-year self-surveillance program Methods: Test strips were routinely sampled from randomly selected manufacturer's production batches and sent to one of 3 clinic sites for clinical accuracy assessment using fresh capillary blood from patients with diabetes, using both the meter system and standard laboratory reference instrument. Evaluation of the distribution of strip batch MARD yielded a mean value of 5.05% (range: 3.68-6.43% at ±1.96 standard deviations from mean). The overall MARD for all clinic data points (N = 80 258) was also 5.05%, while a mean bias of 1.28 was recorded. MARD by glucose level was found to be consistent, yielding a maximum value of 4.81% at higher glucose (≥100 mg/dL) and a mean absolute difference (MAD) of 5.60 mg/dL at low glucose (<100 mg/dL). MARD by year of manufacture varied from 4.67-5.42% indicating consistent accuracy performance over the surveillance period. This 7.5-year surveillance program showed that this meter system exhibits consistently low MARD by batch, glucose level and year, indicating close agreement with established reference methods whilste exhibiting lower MARD values than continuous glucose monitoring (CGM) systems and providing users with confidence in the performance when transitioning to each new strip batch.

  11. Multivariate statistical process control in product quality review assessment - A case study.

    PubMed

    Kharbach, M; Cherrah, Y; Vander Heyden, Y; Bouklouze, A

    2017-11-01

    According to the Food and Drug Administration and the European Good Manufacturing Practices (GMP) guidelines, Annual Product Review (APR) is a mandatory requirement in GMP. It consists of evaluating a large collection of qualitative or quantitative data in order to verify the consistency of an existing process. According to the Code of Federal Regulation Part 11 (21 CFR 211.180), all finished products should be reviewed annually for the quality standards to determine the need of any change in specification or manufacturing of drug products. Conventional Statistical Process Control (SPC) evaluates the pharmaceutical production process by examining only the effect of a single factor at the time using a Shewhart's chart. It neglects to take into account the interaction between the variables. In order to overcome this issue, Multivariate Statistical Process Control (MSPC) can be used. Our case study concerns an APR assessment, where 164 historical batches containing six active ingredients, manufactured in Morocco, were collected during one year. Each batch has been checked by assaying the six active ingredients by High Performance Liquid Chromatography according to European Pharmacopoeia monographs. The data matrix was evaluated both by SPC and MSPC. The SPC indicated that all batches are under control, while the MSPC, based on Principal Component Analysis (PCA), for the data being either autoscaled or robust scaled, showed four and seven batches, respectively, out of the Hotelling T 2 95% ellipse. Also, an improvement of the capability of the process is observed without the most extreme batches. The MSPC can be used for monitoring subtle changes in the manufacturing process during an APR assessment. Copyright © 2017 Académie Nationale de Pharmacie. Published by Elsevier Masson SAS. All rights reserved.

  12. A framework supporting the development of a Grid portal for analysis based on ROI.

    PubMed

    Ichikawa, K; Date, S; Kaishima, T; Shimojo, S

    2005-01-01

    In our research on brain function analysis, users require two different simultaneous types of processing: interactive processing to a specific part of data and high-performance batch processing to an entire dataset. The difference between these two types of processing is in whether or not the analysis is for data in the region of interest (ROI). In this study, we propose a Grid portal that has a mechanism to freely assign computing resources to the users on a Grid environment according to the users' two different types of processing requirements. We constructed a Grid portal which integrates interactive processing and batch processing by the following two mechanisms. First, a job steering mechanism controls job execution based on user-tagged priority among organizations with heterogeneous computing resources. Interactive jobs are processed in preference to batch jobs by this mechanism. Second, a priority-based result delivery mechanism that administrates a rank of data significance. The portal ensures a turn-around time of interactive processing by the priority-based job controlling mechanism, and provides the users with quality of services (QoS) for interactive processing. The users can access the analysis results of interactive jobs in preference to the analysis results of batch jobs. The Grid portal has also achieved high-performance computation of MEG analysis with batch processing on the Grid environment. The priority-based job controlling mechanism has been realized to freely assign computing resources to the users' requirements. Furthermore the achievement of high-performance computation contributes greatly to the overall progress of brain science. The portal has thus made it possible for the users to flexibly include the large computational power in what they want to analyze.

  13. Bacteriophage PRD1 batch experiments to study attachment, detachment and inactivation processes.

    PubMed

    Sadeghi, Gholamreza; Schijven, Jack F; Behrends, Thilo; Hassanizadeh, S Majid; van Genuchten, Martinus Th

    2013-09-01

    Knowledge of virus removal in subsurface environments is pivotal for assessing the risk of viral contamination of water resources and developing appropriate protection measures. Columns packed with sand are frequently used to quantify attachment, detachment and inactivation rates of viruses. Since column transport experiments are very laborious, a common alternative is to perform batch experiments where usually one or two measurements are done assuming equilibrium is reached. It is also possible to perform kinetic batch experiments. In that case, however, it is necessary to monitor changes in the concentration with time. This means that kinetic batch experiments will be almost as laborious as column experiments. Moreover, attachment and detachment rate coefficients derived from batch experiments may differ from those determined using column experiments. The aim of this study was to determine the utility of kinetic batch experiments and investigate the effects of different designs of the batch experiments on estimated attachment, detachment and inactivation rate coefficients. The experiments involved various combinations of container size, sand-water ratio, and mixing method (i.e., rolling or tumbling by pivoting the tubes around their horizontal or vertical axes, respectively). Batch experiments were conducted with clean quartz sand, water at pH 7 and ionic strength of 20 mM, and using the bacteriophage PRD1 as a model virus. Values of attachment, detachment and inactivation rate coefficients were found by fitting an analytical solution of the kinetic model equations to the data. Attachment rate coefficients were found to be systematically higher under tumbling than under rolling conditions because of better mixing and more efficient contact of phages with the surfaces of the sand grains. In both mixing methods, more sand in the container yielded higher attachment rate coefficients. A linear increase in the detachment rate coefficient was observed with increased solid-water ratio using tumbling method. Given the differences in the attachment rate coefficients, and assuming the same sticking efficiencies since chemical conditions of the batch and column experiments were the same, our results show that collision efficiencies of batch experiments are not the same as those of column experiments. Upscaling of the attachment rate from batch to column experiments hence requires proper understanding of the mixing conditions. Because batch experiments, in which the kinetics are monitored, are as laborious as column experiments, there seems to be no major advantage in performing batch instead of column experiments. Copyright © 2013 Elsevier B.V. All rights reserved.

  14. Immuno-Navigator, a batch-corrected coexpression database, reveals cell type-specific gene networks in the immune system

    PubMed Central

    Vandenbon, Alexis; Dinh, Viet H.; Mikami, Norihisa; Kitagawa, Yohko; Teraguchi, Shunsuke; Ohkura, Naganari; Sakaguchi, Shimon

    2016-01-01

    High-throughput gene expression data are one of the primary resources for exploring complex intracellular dynamics in modern biology. The integration of large amounts of public data may allow us to examine general dynamical relationships between regulators and target genes. However, obstacles for such analyses are study-specific biases or batch effects in the original data. Here we present Immuno-Navigator, a batch-corrected gene expression and coexpression database for 24 cell types of the mouse immune system. We systematically removed batch effects from the underlying gene expression data and showed that this removal considerably improved the consistency between inferred correlations and prior knowledge. The data revealed widespread cell type-specific correlation of expression. Integrated analysis tools allow users to use this correlation of expression for the generation of hypotheses about biological networks and candidate regulators in specific cell types. We show several applications of Immuno-Navigator as examples. In one application we successfully predicted known regulators of importance in naturally occurring Treg cells from their expression correlation with a set of Treg-specific genes. For one high-scoring gene, integrin β8 (Itgb8), we confirmed an association between Itgb8 expression in forkhead box P3 (Foxp3)-positive T cells and Treg-specific epigenetic remodeling. Our results also suggest that the regulation of Treg-specific genes within Treg cells is relatively independent of Foxp3 expression, supporting recent results pointing to a Foxp3-independent component in the development of Treg cells. PMID:27078110

  15. Stochastic growth logistic model with aftereffect for batch fermentation process

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Rosli, Norhayati; Ayoubi, Tawfiqullah; Bahar, Arifah

    2014-06-19

    In this paper, the stochastic growth logistic model with aftereffect for the cell growth of C. acetobutylicum P262 and Luedeking-Piret equations for solvent production in batch fermentation system is introduced. The parameters values of the mathematical models are estimated via Levenberg-Marquardt optimization method of non-linear least squares. We apply Milstein scheme for solving the stochastic models numerically. The effciency of mathematical models is measured by comparing the simulated result and the experimental data of the microbial growth and solvent production in batch system. Low values of Root Mean-Square Error (RMSE) of stochastic models with aftereffect indicate good fits.

  16. Stochastic growth logistic model with aftereffect for batch fermentation process

    NASA Astrophysics Data System (ADS)

    Rosli, Norhayati; Ayoubi, Tawfiqullah; Bahar, Arifah; Rahman, Haliza Abdul; Salleh, Madihah Md

    2014-06-01

    In this paper, the stochastic growth logistic model with aftereffect for the cell growth of C. acetobutylicum P262 and Luedeking-Piret equations for solvent production in batch fermentation system is introduced. The parameters values of the mathematical models are estimated via Levenberg-Marquardt optimization method of non-linear least squares. We apply Milstein scheme for solving the stochastic models numerically. The effciency of mathematical models is measured by comparing the simulated result and the experimental data of the microbial growth and solvent production in batch system. Low values of Root Mean-Square Error (RMSE) of stochastic models with aftereffect indicate good fits.

  17. NDA Batch 2002-13

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Hollister, R

    QC sample results (daily background check drum and 100-gram SGS check drum) were within acceptance criteria established by WIPP's Quality Assurance Objectives for TRU Waste Characterization. Replicate runs were performed on drum LL85501243TRU. Replicate measurement results are identical at the 95% confidence level as established by WIPP criteria. HWM NCAR No. 02-1000168 issued on 17-Oct-2002 regarding a partially dislodged Cd sheet filter on the HPGe coaxial detector. This physical geometry occurred on 01-Oct-2002 and was not corrected until 10-Oct-2002, during which period is inclusive of the present batch run of drums. Per discussions among the Independent Technical Reviewer, Expert Reviewermore » and the Technical QA Supervisor, as well as in consultation with John Fleissner, Technical Point of Contact from Canberra, the analytical results are technically reliable. All QC standard runs during this period were in control. Data packet for SGS Batch 2002-13 generated using passive gamma-ray spectroscopy with the Pu Facility SGS unit is technically reasonable. All QC samples are in compliance with establiShed control limits. The batch data packet has been reviewed for correctness, completeness, consistency and compliance with WIPP's Quality Assurance Objectives and determined to be acceptable.« less

  18. A retrospective audit of bacterial culture results of donated human milk in Perth, Western Australia.

    PubMed

    Almutawif, Yahya; Hartmann, Benjamin; Lloyd, Megan; Erber, Wendy; Geddes, Donna

    2017-02-01

    The bacterial content of donated human milk is either endogenous or introduced via contamination. Defining milk bank bacterial content will allow researchers to devise appropriate tests for significant and commonly encountered organisms. A retrospective audit was conducted on data recorded from the Perron Rotary Express Milk Bank, King Edward Memorial Hospital, Subiaco, Western Australia. This aimed to describe the incidence of bacterial species detected in donated human milk and to identify potentially pathogenic bacteria. The data comprised of 2890 batches donated by 448 women between 2007 and 2011. Coagulase negative Staphylococcus (CoNS) represented the highest prevalence of bacteria in donated milk, isolated from 85.5% of batches (range: 20 to 650,000CFU/mL) followed by Acinetobacter species in 8.1% of batches (range: 100 to 180,000CFU/mL). Staphylococcus aureus was the most prevalent potentially pathogenic bacteria in 5% of batches (range: 40 to 100,000CFU/mL). Further investigation is warranted to better define the risks posed by the presence of toxin-producing S. aureus in raw and pasteurized human milk which may allow minimization of risk to the preterm infants. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

  19. Batch and fixed-bed column studies for biosorption of Zn(II) ions onto pongamia oil cake (Pongamia pinnata) from biodiesel oil extraction.

    PubMed

    Shanmugaprakash, M; Sivakumar, V

    2015-12-01

    The present work, analyzes the potential of defatted pongamia oil cake (DPOC) for the biosorption of Zn(II) ions from aqueous solutions in the both batch and column mode. Batch experiments were conducted to evaluate the optimal pH, effect of adsorbent dosage, initial Zn(II) ions concentration and contact time. The biosorption equilibrium and kinetics data for Zn(II) ions onto the DPOC were studied in detail, using several models, among all it was found to be that, Freundlich and the second-order model explained the equilibrium data well. The calculated thermodynamic parameters had shown that the biosorption of Zn(II) ions was exothermic and spontaneous in nature. Batch desorption studies showed that the maximum Zn(II) recovery occurred, using 0.1 M EDTA. The Bed Depth Service Time (BDST) and the Thomas model was successfully employed to evaluate the model parameters in the column mode. The results indicated that the DPOC can be applied as an effective and eco-friendly biosorbent for the removal of Zn(II) ions in polluted wastewater. Copyright © 2015 Elsevier Ltd. All rights reserved.

  20. Verification Of The Defense Waste Processing Facility's (DWPF) Process Digestion Methods For The Sludge Batch 8 Qualification Sample

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Click, D. R.; Edwards, T. B.; Wiedenman, B. J.

    2013-03-18

    This report contains the results and comparison of data generated from inductively coupled plasma – atomic emission spectroscopy (ICP-AES) analysis of Aqua Regia (AR), Sodium Peroxide/Sodium Hydroxide Fusion Dissolution (PF) and Cold Chem (CC) method digestions and Cold Vapor Atomic Absorption analysis of Hg digestions from the DWPF Hg digestion method of Sludge Batch 8 (SB8) Sludge Receipt and Adjustment Tank (SRAT) Receipt and SB8 SRAT Product samples. The SB8 SRAT Receipt and SB8 SRAT Product samples were prepared in the SRNL Shielded Cells, and the SRAT Receipt material is representative of the sludge that constitutes the SB8 Batch ormore » qualification composition. This is the sludge in Tank 51 that is to be transferred into Tank 40, which will contain the heel of Sludge Batch 7b (SB7b), to form the SB8 Blend composition.« less

  1. Fuzzy logic feedback control for fed-batch enzymatic hydrolysis of lignocellulosic biomass.

    PubMed

    Tai, Chao; Voltan, Diego S; Keshwani, Deepak R; Meyer, George E; Kuhar, Pankaj S

    2016-06-01

    A fuzzy logic feedback control system was developed for process monitoring and feeding control in fed-batch enzymatic hydrolysis of a lignocellulosic biomass, dilute acid-pretreated corn stover. Digested glucose from hydrolysis reaction was assigned as input while doser feeding time and speed of pretreated biomass were responses from fuzzy logic control system. Membership functions for these three variables and rule-base were created based on batch hydrolysis data. The system response was first tested in LabVIEW environment then the performance was evaluated through real-time hydrolysis reaction. The feeding operations were determined timely by fuzzy logic control system and efficient responses were shown to plateau phases during hydrolysis. Feeding of proper amount of cellulose and maintaining solids content was well balanced. Fuzzy logic proved to be a robust and effective online feeding control tool for fed-batch enzymatic hydrolysis.

  2. Effects of HPMC substituent pattern on water up-take, polymer and drug release: An experimental and modelling study.

    PubMed

    Caccavo, Diego; Lamberti, Gaetano; Barba, Anna Angela; Abrahmsén-Alami, Susanna; Viridén, Anna; Larsson, Anette

    2017-08-07

    The purpose of this study was to investigate the hydration behavior of two matrix formulations containing the cellulose derivative hydroxypropyl methylcellulose (HPMC). The two HPMC batches investigated had different substitution pattern along the backbone; the first one is referred to as heterogeneous and the second as homogenous. The release of both the drug molecule theophylline and the polymer was determined. Additionally, the water concentrations at different positions in the swollen gel layers were determined by Magnetic Resonance Imaging. The experimental data was compared to predicted values obtained by the extension of a mechanistic Fickian based model. The hydration of tablets containing the more homogenous HPMC batch showed a gradual water concentration gradient in the gel layer and could be well predicted. The hydration process for the more heterogeneous batch showed a very abrupt step change in the water concentration in the gel layer and could not be well predicted. Based on the comparison between the experimental and predicted data this study suggests, for the first time, that formulations with HPMC of different heterogeneities form gels in different ways. The homogeneous HPMC batch exhibits a water sorption behavior ascribable to a Ficḱs law for the diffusion process whereas the more heterogeneous HPMC batches does not. This conclusion is important in the future development of simulation models and in the understanding of drug release mechanism from hydrophilic matrices. Copyright © 2017 Elsevier B.V. All rights reserved.

  3. Large-scale manufacturing of GMP-compliant anti-EGFR targeted nanocarriers: production of doxorubicin-loaded anti-EGFR-immunoliposomes for a first-in-man clinical trial.

    PubMed

    Wicki, Andreas; Ritschard, Reto; Loesch, Uli; Deuster, Stefanie; Rochlitz, Christoph; Mamot, Christoph

    2015-04-30

    We describe the large-scale, GMP-compliant production process of doxorubicin-loaded and anti-EGFR-coated immunoliposomes (anti-EGFR-ILs-dox) used in a first-in-man, dose escalation clinical trial. 10 batches of this nanoparticle have been produced in clean room facilities. Stability data from the pre-GMP and the GMP batch indicate that the anti-EGFR-ILs-dox nanoparticle was stable for at least 18 months after release. Release criteria included visual inspection, sterility testing, as well as measurements of pH (pH 5.0-7.0), doxorubicin HCl concentration (0.45-0.55 mg/ml), endotoxin concentration (<1.21 IU/ml), leakage (<10%), particle size (Z-average of Caelyx ± 20 nm), and particle uptake (uptake absolute: >0.50 ng doxorubicin/μg protein; uptake relatively to PLD: >5 fold). All batches fulfilled the defined release criteria, indicating a high reproducibility as well as batch-to-batch uniformity of the main physico-chemical features of the nanoparticles in the setting of the large-scale GMP process. In the clinical trial, 29 patients were treated with this nanoparticle between 2007 and 2010. Pharmacokinetic data of anti-EGFR-ILs-dox collected during the clinical study revealed stability of the nanocarrier in vivo. Thus, reliable and GMP-compliant production of anti-EGFR-targeted nanoparticles for clinical application is feasible. Copyright © 2015 Elsevier B.V. All rights reserved.

  4. Comparison Between Individually and Group-Based Insulin Pump Initiation by Time-Driven Activity-Based Costing

    PubMed Central

    Ridderstråle, Martin

    2017-01-01

    Background: Depending on available resources, competencies, and pedagogic preference, initiation of insulin pump therapy can be performed on either an individual or a group basis. Here we compared the two models with respect to resources used. Methods: Time-driven activity-based costing (TDABC) was used to compare initiating insulin pump treatment in groups (GT) to individual treatment (IT). Activities and cost drivers were identified, timed, or estimated at location. Medical quality and patient satisfaction were assumed to be noninferior and were not measured. Results: GT was about 30% less time-consuming and 17% less cost driving per patient and activity compared to IT. As a batch driver (16 patients in one group) GT produced an upward jigsaw-shaped accumulative cost curve compared to the incremental increase incurred by IT. Taking the alternate cost for those not attending into account, and realizing the cost of opportunity gained, suggested that GT was cost neutral already when 5 of 16 patients attended, and that a second group could be initiated at no additional cost as the attendance rate reached 15:1. Conclusions: We found TDABC to be effective in comparing treatment alternatives, improving cost control and decision making. Everything else being equal, if the setup is available, our data suggest that initiating insulin pump treatment in groups is far more cost effective than on an individual basis and that TDABC may be used to find the balance point. PMID:28366085

  5. FDA 2011 process validation guidance: lifecycle compliance model.

    PubMed

    Campbell, Cliff

    2014-01-01

    This article has been written as a contribution to the industry's efforts in migrating from a document-driven to a data-driven compliance mindset. A combination of target product profile, control engineering, and general sum principle techniques is presented as the basis of a simple but scalable lifecycle compliance model in support of modernized process validation. Unit operations and significant variables occupy pole position within the model, documentation requirements being treated as a derivative or consequence of the modeling process. The quality system is repositioned as a subordinate of system quality, this being defined as the integral of related "system qualities". The article represents a structured interpretation of the U.S. Food and Drug Administration's 2011 Guidance for Industry on Process Validation and is based on the author's educational background and his manufacturing/consulting experience in the validation field. The U.S. Food and Drug Administration's Guidance for Industry on Process Validation (2011) provides a wide-ranging and rigorous outline of compliant drug manufacturing requirements relative to its 20(th) century predecessor (1987). Its declared focus is patient safety, and it identifies three inter-related (and obvious) stages of the compliance lifecycle. Firstly, processes must be designed, both from a technical and quality perspective. Secondly, processes must be qualified, providing evidence that the manufacturing facility is fully "roadworthy" and fit for its intended purpose. Thirdly, processes must be verified, meaning that commercial batches must be monitored to ensure that processes remain in a state of control throughout their lifetime.

  6. Recent volcanic history of Irazu volcano, Costa Rica: alternation and mixing of two magma batches, and pervasive mixing

    USGS Publications Warehouse

    Alvarado, Guillermo E.; Carr, Michael J.; Turrin, Brent D.; Swisher, Carl C.; Schmincke, Hans-Ulrich; Hudnut, Kenneth W.

    2006-01-01

    40Ar/39Ar dates, field observations, and geochemical data are reported for Irazú volcano, Costa Rica. Volcanism dates back to at least 854 ka, but has been episodic with lava shield construction peaks at ca. 570 ka and 136–0 ka. The recent volcanic record on Irazú volcano comprises lava flows and a variety of Strombolian and phreatomagmatic deposits, with a long-term trend toward more hydrovolcanic deposits. Banded scorias and hybridized rocks reflect ubiquitous magma mixing and commingling. Two distinct magma batches have been identified. One magma type or batch, Haya, includes basalt with higher high field strength (HFS) and rare-earth element contents, suggesting a lower degree melt of a subduction modified mantle source. The second batch, Sapper, has greater enrichment of large ion lithophile elements (LILE) relative to HFS elements and rare-earth elements, suggesting a higher subduction signature. The recent volcanic history at Irazú records two and one half sequences of the following pattern: eruptions of the Haya batch; eruptions of the Sapper batch; and finally, an unusually clear unconformity, indicating a pause in eruptions. In the last two sequences, strongly hybridized magma erupted after the eruption of the Haya batch. The continuing presence of two distinct magma batches requires two active magma chambers. The common occurrence of hybrids is evidence for a small, nearer to the surface chamber for mixing the two batches. Estimated pre-eruptive temperatures based on two-pyroxene geothermometry range from ∼1000–1176 °C in basalts to 922 °C in hornblende andesites. Crystallization occurred mainly between 4.6 and 3 kb as measured by different geobarometers. Hybridized rocks show intermediate pressures and temperatures. High silica magma occurs in very small volumes as banded scorias but not as lava flows. Although eruptions at Irazú are not often very explosive, the pervasiveness of magma mixing presents the danger of larger, more explosive hybrid eruptions.

  7. A Modified Edge Crack Torsion Test for Measurement of Mode III Fracture Toughness of Laminated Tape Composites

    NASA Technical Reports Server (NTRS)

    Czabaj, Michael W.; Davidson, Barry D.; Ratcliffe, James G.

    2016-01-01

    Modifications to the edge crack torsion (ECT) test are studied to improve the reliability of this test for measuring the mode-III fracture toughness, G (sub IIIc), of laminated tape fiber-reinforced polymeric (FRP) composites. First, the data reduction methods currently used in the ECT test are evaluated and deficiencies in their accuracy are discussed. An alternative data reduction technique, which uses a polynomial form to represent ECT specimen compliance solution, is evaluated and compared to FEA (finite element analysis) results. Second, seven batches of ECT specimens are tested, each batch containing specimens with a preimplanted midplane edge delamination and midplane plies with orientations of plus theta divided by minus theta, with theta ranging from 0 degrees to 90 degrees in 15-degree increments. Tests on these specimens show that intralaminar cracking occurs in specimens from all batches except for which theta = 15 degrees and 30 degrees. Tests on specimens of these two batches are shown to result in mode-III delamination growth at the intended ply interface. The findings from this study are encouraging steps towards the use of the ECT test as a standardized method for measuring G (sub IIIc), although further modification to the data reduction method is required to make it suitable for use as part of a standardized test method.

  8. Cloud Bursting with GlideinWMS: Means to satisfy ever increasing computing needs for Scientific Workflows

    NASA Astrophysics Data System (ADS)

    Mhashilkar, Parag; Tiradani, Anthony; Holzman, Burt; Larson, Krista; Sfiligoi, Igor; Rynge, Mats

    2014-06-01

    Scientific communities have been in the forefront of adopting new technologies and methodologies in the computing. Scientific computing has influenced how science is done today, achieving breakthroughs that were impossible to achieve several decades ago. For the past decade several such communities in the Open Science Grid (OSG) and the European Grid Infrastructure (EGI) have been using GlideinWMS to run complex application workflows to effectively share computational resources over the grid. GlideinWMS is a pilot-based workload management system (WMS) that creates on demand, a dynamically sized overlay HTCondor batch system on grid resources. At present, the computational resources shared over the grid are just adequate to sustain the computing needs. We envision that the complexity of the science driven by "Big Data" will further push the need for computational resources. To fulfill their increasing demands and/or to run specialized workflows, some of the big communities like CMS are investigating the use of cloud computing as Infrastructure-As-A-Service (IAAS) with GlideinWMS as a potential alternative to fill the void. Similarly, communities with no previous access to computing resources can use GlideinWMS to setup up a batch system on the cloud infrastructure. To enable this, the architecture of GlideinWMS has been extended to enable support for interfacing GlideinWMS with different Scientific and commercial cloud providers like HLT, FutureGrid, FermiCloud and Amazon EC2. In this paper, we describe a solution for cloud bursting with GlideinWMS. The paper describes the approach, architectural changes and lessons learned while enabling support for cloud infrastructures in GlideinWMS.

  9. Access to small size distributions of nanoparticles by microwave-assisted synthesis. Formation of Ag nanoparticles in aqueous carboxymethylcellulose solutions in batch and continuous-flow reactors

    NASA Astrophysics Data System (ADS)

    Horikoshi, Satoshi; Abe, Hideki; Torigoe, Kanjiro; Abe, Masahiko; Serpone, Nick

    2010-08-01

    This article examines the effect(s) of the 2.45-GHz microwave (MW) radiation in the synthesis of silver nanoparticles in aqueous media by reduction of the diaminesilver(i) complex, [Ag(NH3)2]+, with carboxymethylcellulose (CMC) in both batch-type and continuous-flow reactor systems with a particular emphasis on the characteristics of the microwaves in this process and the size distributions. This microwave thermally-assisted synthesis is compared to a conventional heating (CH) method, both requiring a reaction temperature of 100 °C to produce the nanoparticles, in both cases leading to the formation of silver colloids with different size distributions. Reduction of the diaminesilver(i) precursor complex, [Ag(NH3)2]+, by CMC depended on the solution temperature. Cooling the reactor during the heating process driven with 390-Watt microwaves (MW-390W/Cool protocol) yielded silver nanoparticles with sizes spanning the range 1-2 nm. By contrast, the size distribution of Ag nanoparticles with 170-Watt microwaves (no cooling; MW-170W protocol) was in the range 1.4-3.6 nm (average size ~3 nm). The overall results suggest the potential for a scale-up process in the microwave-assisted synthesis of nanoparticles. Based on the present data, a flow-through microwave reactor system is herein proposed for the continuous production of silver nanoparticles. The novel flow reactor system (flow rate, 600 mL min-1) coupled to 1200-Watt microwave radiation generated silver nanoparticles with a size distribution 0.7-2.8 nm (average size ca. 1.5 nm).

  10. Cloud Bursting with GlideinWMS: Means to satisfy ever increasing computing needs for Scientific Workflows

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Mhashilkar, Parag; Tiradani, Anthony; Holzman, Burt

    Scientific communities have been in the forefront of adopting new technologies and methodologies in the computing. Scientific computing has influenced how science is done today, achieving breakthroughs that were impossible to achieve several decades ago. For the past decade several such communities in the Open Science Grid (OSG) and the European Grid Infrastructure (EGI) have been using GlideinWMS to run complex application workflows to effectively share computational resources over the grid. GlideinWMS is a pilot-based workload management system (WMS) that creates on demand, a dynamically sized overlay HTCondor batch system on grid resources. At present, the computational resources shared overmore » the grid are just adequate to sustain the computing needs. We envision that the complexity of the science driven by 'Big Data' will further push the need for computational resources. To fulfill their increasing demands and/or to run specialized workflows, some of the big communities like CMS are investigating the use of cloud computing as Infrastructure-As-A-Service (IAAS) with GlideinWMS as a potential alternative to fill the void. Similarly, communities with no previous access to computing resources can use GlideinWMS to setup up a batch system on the cloud infrastructure. To enable this, the architecture of GlideinWMS has been extended to enable support for interfacing GlideinWMS with different Scientific and commercial cloud providers like HLT, FutureGrid, FermiCloud and Amazon EC2. In this paper, we describe a solution for cloud bursting with GlideinWMS. The paper describes the approach, architectural changes and lessons learned while enabling support for cloud infrastructures in GlideinWMS.« less

  11. 3D magnetohydrodynamic modelling of a dc low-current plasma arc batch reactor at very high pressure in helium

    NASA Astrophysics Data System (ADS)

    Lebouvier, A.; Iwarere, S. A.; Ramjugernath, D.; Fulcheri, L.

    2013-04-01

    This paper deals with a three-dimensional (3D) time-dependent magnetohydrodynamic (MHD) model under peculiar conditions of very high pressures (from 2 MPa up to 10 MPa) and low currents (<1 A). Studies on plasma arc working under these unusual conditions remain almost unexplored because of the technical and technological challenges to develop a reactor able to sustain a plasma at very high pressures. The combined effect of plasma reactivity and high pressure would probably open the way towards new promising applications in various fields: chemistry, lightning, materials or nanomaterial synthesis. A MHD model helps one to understand the complex and coupled phenomena surrounding the plasma which cannot be understood by simply experimentation. The model also provides data which are difficult to directly determine experimentally. The model simulates an experimental-based batch reactor working with helium. The particular reactor in question was used to investigate the Fischer-Tropsch application, fluorocarbon production and CO2 retro-conversion. However, as a first approach in terms of MHD, the model considers the case for helium as a non-reactive working gas. After a detailed presentation of the model, a reference case has been fully analysed (P = 8 MPa, I = 0.35 A) in terms of physical properties. The results show a bending of the arc and displacement of the anodic arc root towards the top of the reactor, due to the combined effects of convection, gravity and electromagnetic forces. A parametric study on the pressure (2-10 MPa) and current (0.25-0.4 A) was then investigated. The operating pressure does not show an influence on the contraction of the arc but higher pressures involve a higher natural convection in the reactor, driven by the density gradients between the cold and hot gas.

  12. Interactive computer methods for generating mineral-resource maps

    USGS Publications Warehouse

    Calkins, James Alfred; Crosby, A.S.; Huffman, T.E.; Clark, A.L.; Mason, G.T.; Bascle, R.J.

    1980-01-01

    Inasmuch as maps are a basic tool of geologists, the U.S. Geological Survey's CRIB (Computerized Resources Information Bank) was constructed so that the data it contains can be used to generate mineral-resource maps. However, by the standard methods used-batch processing and off-line plotting-the production of a finished map commonly takes 2-3 weeks. To produce computer-generated maps more rapidly, cheaply, and easily, and also to provide an effective demonstration tool, we have devised two related methods for plotting maps as alternatives to conventional batch methods. These methods are: 1. Quick-Plot, an interactive program whose output appears on a CRT (cathode-ray-tube) device, and 2. The Interactive CAM (Cartographic Automatic Mapping system), which combines batch and interactive runs. The output of the Interactive CAM system is final compilation (not camera-ready) paper copy. Both methods are designed to use data from the CRIB file in conjunction with a map-plotting program. Quick-Plot retrieves a user-selected subset of data from the CRIB file, immediately produces an image of the desired area on a CRT device, and plots data points according to a limited set of user-selected symbols. This method is useful for immediate evaluation of the map and for demonstrating how trial maps can be made quickly. The Interactive CAM system links the output of an interactive CRIB retrieval to a modified version of the CAM program, which runs in the batch mode and stores plotting instructions on a disk, rather than on a tape. The disk can be accessed by a CRT, and, thus, the user can view and evaluate the map output on a CRT immediately after a batch run, without waiting 1-3 days for an off-line plot. The user can, therefore, do most of the layout and design work in a relatively short time by use of the CRT, before generating a plot tape and having the map plotted on an off-line plotter.

  13. Knowledge management in secondary pharmaceutical manufacturing by mining of data historians-A proof-of-concept study.

    PubMed

    Meneghetti, Natascia; Facco, Pierantonio; Bezzo, Fabrizio; Himawan, Chrismono; Zomer, Simeone; Barolo, Massimiliano

    2016-05-30

    In this proof-of-concept study, a methodology is proposed to systematically analyze large data historians of secondary pharmaceutical manufacturing systems using data mining techniques. The objective is to develop an approach enabling to automatically retrieve operation-relevant information that can assist the management in the periodic review of a manufactory system. The proposed methodology allows one to automatically perform three tasks: the identification of single batches within the entire data-sequence of the historical dataset, the identification of distinct operating phases within each batch, and the characterization of a batch with respect to an assigned multivariate set of operating characteristics. The approach is tested on a six-month dataset of a commercial-scale granulation/drying system, where several millions of data entries are recorded. The quality of results and the generality of the approach indicate that there is a strong potential for extending the method to even larger historical datasets and to different operations, thus making it an advanced PAT tool that can assist the implementation of continual improvement paradigms within a quality-by-design framework. Copyright © 2016 Elsevier B.V. All rights reserved.

  14. Modelling of Batch Lactic Acid Fermentation in
the Presence of Anionic Clay

    PubMed Central

    Jinescu, Cosmin; Aruş, Vasilica Alisa; Nistor, Ileana Denisa

    2014-01-01

    Summary Batch fermentation of milk inoculated with lactic acid bacteria was conducted in the presence of hydrotalcite-type anionic clay under static and ultrasonic conditions. An experimental study of the effect of fermentation temperature (t=38–43 °C), clay/milk ratio (R=1–7.5 g/L) and ultrasonic field (ν=0 and 35 kHz) on process dynamics was performed. A mathematical model was selected to describe the fermentation process kinetics and its parameters were estimated based on experimental data. A good agreement between the experimental and simulated results was achieved. Consequently, the model can be employed to predict the dynamics of batch lactic acid fermentation with values of process variables in the studied ranges. A statistical analysis of the data based on a 23 factorial experiment was performed in order to express experimental and model-regressed process responses depending on t, R and ν factors. PMID:27904318

  15. Improved solution accuracy for Landsat-4 (TDRSS-user) orbit determination

    NASA Technical Reports Server (NTRS)

    Oza, D. H.; Niklewski, D. J.; Doll, C. E.; Mistretta, G. D.; Hart, R. C.

    1994-01-01

    This paper presents the results of a study to compare the orbit determination accuracy for a Tracking and Data Relay Satellite System (TDRSS) user spacecraft, Landsat-4, obtained using a Prototype Filter Smoother (PFS), with the accuracy of an established batch-least-squares system, the Goddard Trajectory Determination System (GTDS). The results of Landsat-4 orbit determination will provide useful experience for the Earth Observing System (EOS) series of satellites. The Landsat-4 ephemerides were estimated for the January 17-23, 1991, timeframe, during which intensive TDRSS tracking data for Landsat-4 were available. Independent assessments were made of the consistencies (overlap comparisons for the batch case and convariances for the sequential case) of solutions produced by the batch and sequential methods. The filtered and smoothed PFS orbit solutions were compared with the definitive GTDS orbit solutions for Landsat-4; the solution differences were generally less than 15 meters.

  16. An Inversion Analysis of Recent Variability in CO2 Fluxes Using GOSAT and In Situ Observations

    NASA Astrophysics Data System (ADS)

    Wang, J. S.; Kawa, S. R.; Baker, D. F.; Collatz, G. J.

    2016-12-01

    About one-half of the global CO2 emissions from fossil fuel combustion and deforestation accumulates in the atmosphere, where it contributes to global warming. The rest is taken up by vegetation and the ocean. The precise contribution of the two sinks and their location and year-to-year variability are not well understood. We use two different approaches, batch Bayesian synthesis inversion and variational data assimilation, to deduce the global spatiotemporal distributions of CO2 fluxes during 2009-2010. One of our objectives is to assess different sources of uncertainties in inferred fluxes, including uncertainties in prior flux estimates and observations, and differences in inversion techniques. For prior constraints, we utilize fluxes and uncertainties from the CASA-GFED model of the terrestrial biosphere and biomass burning driven by satellite observations. We also use measurement-based ocean flux estimates and fixed fossil CO2 emissions. Our inversions incorporate column CO2 measurements from the GOSAT satellite (ACOS retrieval, bias-corrected) and in situ observations (individual flask and afternoon-average continuous observations) to estimate fluxes in 108 regions over 8-day intervals for the batch inversion and at 3° x 3.75° weekly for the variational system. Relationships between fluxes and atmospheric concentrations are derived consistently for the two inversion systems using the PCTM transport model with MERRA meteorology. We compare the posterior fluxes and uncertainties derived using different data sets and the two inversion approaches, and evaluate the posterior atmospheric concentrations against independent data including aircraft measurements. The optimized fluxes generally resemble each other and those from other studies. For example, a GOSAT-only inversion suggests a shift in the global sink from the tropics/south to the north relative to the prior and to an in-situ-only inversion. The posterior fluxes of the GOSAT inversion are better constrained in most regions than those of the in situ inversion because of the greater spatial coverage of the GOSAT observations. The GOSAT inversion also indicates a significantly smaller terrestrial sink in higher-latitude northern regions in boreal summer of 2010 relative to 2009, consistent with observed drought conditions.

  17. [Dissolution behavior of Fuzi Lizhong pill based on simultaneous determination of two components in Glycyrrhizae Radix et Rhizoma].

    PubMed

    Jiang, Mao-Yuan; Zhang, Zhen; Shi, Jin-Feng; Zhang, Jin-Ming; Fu, Chao-Mei; Lin, Xia; Liu, Yu-Mei

    2018-03-01

    To preliminarily investigate the dissolution behavior of Fuzi Lizhong pill, provide the basis for its quality control and lay foundation for in vivo dissolution behavior by determining the dissolution rate of liquiritin and glycyrrhizic acid. High-performance liquid chromatography (HPLC) method for simultaneous content determination of the two active ingredients of liquiritin and glycyrrhizic acid in Fuzi Lizhong pill was established; The dissolution amount of these two active ingredients in fifteen batches of Fuzi Lizhong pill from five manufacturers was obtained at different time points, and then the cumulative dissolution rate was calculated and cumulative dissolution curve was drawn. The similarity of cumulative dissolution curve of different batches was evaluated based on the same factory, and the similarity of cumulative dissolution curve of different factories was evaluated based on the same active ingredients. The dissolution model of Fuzi Lizhong pill based on two kinds of active ingredients was established by fitting with the dissolution data. The best dissolution medium was 0.25% sodium lauryl sulfate. The dissolution behavior of liquiritin and glycyrrhizic acid in Fuzi Lizhong pill was basically the same and sustained release in 48 h. Three batches of the factories (factory 2, factory 3, factory 4 and factory 5) appeared to be similar in dissolution behavior, indicating similarity in dissolution behavior in most factories. Two of the three batches from factory 1 appeared to be not similar in dissolution behavior of liquiritin and glycyrrhizic acid. The dissolution data of the effective ingredients from different factories were same in fitting, and Weibull model was the best model in these batches. Fuzi Lizhong pill in 15 batches from 5 factories showed sustained release in 48 h, proving obviously slow releasing characteristics "pill is lenitive and keeps a long-time efficacy". The generally good dissolution behavior also suggested that quality of different batches from most factories was stable. The dissolution behavior of liquiritin and glycyrrhizic acid in different factories was different, suggesting that the source of medicinal materials and preparation technology parameters in five factories were different. Copyright© by the Chinese Pharmaceutical Association.

  18. Meeting Report: Batch-to-Batch Variability in Estrogenic Activity in Commercial Animal Diets—Importance and Approaches for Laboratory Animal Research

    PubMed Central

    Heindel, Jerrold J.; vom Saal, Frederick S.

    2008-01-01

    We report information from two workshops sponsored by the National Institutes of Health that were held to a) assess whether dietary estrogens could significantly impact end points in experimental animals, and b) involve program participants and feed manufacturers to address the problems associated with measuring and eliminating batch-to-batch variability in rodent diets that may lead to conflicting findings in animal experiments within and between laboratories. Data were presented at the workshops showing that there is significant batch-to-batch variability in estrogenic content of commercial animal diets, and that this variability results in differences in experimental outcomes. A combination of methods were proposed to determine levels of total estrogenic activity and levels of specific estrogenic constituents in soy-containing, casein-containing, and other soy-free rodent diets. Workshop participants recommended that researchers pay greater attention to the type of diet being used in animal studies and choose a diet whose estrogenic activity (or lack thereof) is appropriate for the experimental model and end points of interest. Information about levels of specific phytoestrogens, as well as estrogenic activity caused by other contaminants and measured by bioassay, should be disclosed in scientific publications. This will require laboratory animal diet manufacturers to provide investigators with information regarding the phytoestrogen content and other estrogenic compounds in commercial diets used in animal research. PMID:18335108

  19. Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose.

    PubMed

    Yang, Bin; Wyman, Charles E

    2004-04-05

    Compared with batch systems, flowthrough and countercurrent reactors have important potential advantages for pretreating cellulosic biomass, including higher hemicellulose sugar yields, enhanced cellulose digestibility, and reduced chemical additions. Unfortunately, they suffer from high water and energy use. To better understand these trade-offs, comparative data are reported on xylan and lignin removal and enzymatic digestibility of cellulose for corn stover pretreated in batch and flowthrough reactors over a range of flow rates between 160 degrees and 220 degrees C, with water only and also with 0.1 wt% sulfuric acid. Increasing flow with just water enhanced the xylan dissolution rate, more than doubled total lignin removal, and increased cellulose digestibility. Furthermore, adding dilute sulfuric acid increased the rate of xylan removal for both batch and flowthrough systems. Interestingly, adding acid also increased the lignin removal rate with flow, but less lignin was left in solution when acid was added in batch. Although the enzymatic hydrolysis of pretreated cellulose was related to xylan removal, as others have shown, the digestibility was much better for flowthrough compared with batch systems, for the same degree of xylan removal. Cellulose digestibility for flowthrough reactors was related to lignin removal as well. These results suggest that altering lignin also affects the enzymatic digestibility of corn stover. Copyright 2004 Wiley Periodicals, Inc.

  20. Meeting report: batch-to-batch variability in estrogenic activity in commercial animal diets--importance and approaches for laboratory animal research.

    PubMed

    Heindel, Jerrold J; vom Saal, Frederick S

    2008-03-01

    We report information from two workshops sponsored by the National Institutes of Health that were held to a) assess whether dietary estrogens could significantly impact end points in experimental animals, and b) involve program participants and feed manufacturers to address the problems associated with measuring and eliminating batch-to-batch variability in rodent diets that may lead to conflicting findings in animal experiments within and between laboratories. Data were presented at the workshops showing that there is significant batch-to-batch variability in estrogenic content of commercial animal diets, and that this variability results in differences in experimental outcomes. A combination of methods were proposed to determine levels of total estrogenic activity and levels of specific estrogenic constituents in soy-containing, casein-containing, and other soy-free rodent diets. Workshop participants recommended that researchers pay greater attention to the type of diet being used in animal studies and choose a diet whose estrogenic activity (or lack thereof) is appropriate for the experimental model and end points of interest. Information about levels of specific phytoestrogens, as well as estrogenic activity caused by other contaminants and measured by bioassay, should be disclosed in scientific publications. This will require laboratory animal diet manufacturers to provide investigators with information regarding the phytoestrogen content and other estrogenic compounds in commercial diets used in animal research.

  1. Kinetic characterization and fed-batch fermentation for maximal simultaneous production of esterase and protease from Lysinibacillus fusiformis AU01.

    PubMed

    Divakar, K; Suryia Prabha, M; Nandhinidevi, G; Gautam, P

    2017-04-21

    The simultaneous production of intracellular esterase and extracellular protease from the strain Lysinibacillus fusiformis AU01 was studied in detail. The production was performed both under batch and fed-batch modes. The maximum yield of intracellular esterase and protease was obtained under full oxygen saturation at the beginning of the fermentation. The data were fitted to the Luedeking-Piret model and it was shown that the enzyme (both esterase and protease) production was growth associated. A decrease in intracellular esterase and increase in the extracellular esterase were observed during late stationary phase. The appearance of intracellular proteins in extracellular media and decrease in viable cell count and biomass during late stationary phase confirmed that the presence of extracellular esterase is due to cell lysis. Even though the fed-batch fermentation with different feeding strategies showed improved productivity, feeding yeast extract under DO-stat fermentation conditions showed highest intracellular esterase and protease production. Under DO-stat fed-batch cultivation, maximum intracellular esterase activity of 820 × 10 3 U/L and extracellular protease activity of 172 × 10 3 U/L were obtained at the 16th hr. Intracellular esterase and extracellular protease production were increased fivefold and fourfold, respectively, when compared to batch fermentation performed under shake flask conditions.

  2. DEVELOPMENT OF AN ANTIFOAM TRACKING SYSTEM AS AN OPTION TO SUPPORT THE MELTER OFF-GAS FLAMMABILITY CONTROL STRATEGY AT THE DWPF

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Edwards, T.; Lambert, D.

    The Savannah River National Laboratory (SRNL) has been working with the Savannah River Remediation (SRR) Defense Waste Processing Facility (DWPF) in the development and implementation of an additional strategy for confidently satisfying the flammability controls for DWPF’s melter operation. An initial strategy for implementing the operational constraints associated with flammability control in DWPF was based upon an analytically determined carbon concentration from antifoam. Due to the conservative error structure associated with the analytical approach, its implementation has significantly reduced the operating window for processing and has led to recurrent Slurry Mix Evaporator (SME) and Melter Feed Tank (MFT) remediation. Tomore » address the adverse operating impact of the current implementation strategy, SRR issued a Technical Task Request (TTR) to SRNL requesting the development and documentation of an alternate strategy for evaluating the carbon contribution from antifoam. The proposed strategy presented in this report was developed under the guidance of a Task Technical and Quality Assurance Plan (TTQAP) and involves calculating the carbon concentration from antifoam based upon the actual mass of antifoam added to the process assuming 100% retention. The mass of antifoam in the Additive Mix Feed Tank (AMFT), in the Sludge Receipt and Adjustment Tank (SRAT), and in the SME is tracked by mass balance as part of this strategy. As these quantities are monitored, the random and bias uncertainties affecting their values are also maintained and accounted for. This report documents: 1) the development of an alternate implementation strategy and associated equations describing the carbon concentration from antifoam in each SME batch derived from the actual amount of antifoam introduced into the AMFT, SRAT, and SME during the processing of the batch. 2) the equations and error structure for incorporating the proposed strategy into melter off-gas flammability assessments. Sample calculations of the system are also included in this report. Please note that the system developed and documented in this report is intended as an alternative to the current, analytically-driven system being utilized by DWPF; the proposed system is not intended to eliminate the current system. Also note that the system developed in this report to track antifoam mass in the AMFT, SRAT, and SME will be applicable beyond just Sludge Batch 8. While the model used to determine acceptability of the SME product with respect to melter off-gas flammability controls must be reassessed for each change in sludge batch, the antifoam mass tracking methodology is independent of sludge batch composition and as such will be transferable to future sludge batches.« less

  3. A high-throughput media design approach for high performance mammalian fed-batch cultures

    PubMed Central

    Rouiller, Yolande; Périlleux, Arnaud; Collet, Natacha; Jordan, Martin; Stettler, Matthieu; Broly, Hervé

    2013-01-01

    An innovative high-throughput medium development method based on media blending was successfully used to improve the performance of a Chinese hamster ovary fed-batch medium in shaking 96-deepwell plates. Starting from a proprietary chemically-defined medium, 16 formulations testing 43 of 47 components at 3 different levels were designed. Media blending was performed following a custom-made mixture design of experiments considering binary blends, resulting in 376 different blends that were tested during both cell expansion and fed-batch production phases in one single experiment. Three approaches were chosen to provide the best output of the large amount of data obtained. A simple ranking of conditions was first used as a quick approach to select new formulations with promising features. Then, prediction of the best mixes was done to maximize both growth and titer using the Design Expert software. Finally, a multivariate analysis enabled identification of individual potential critical components for further optimization. Applying this high-throughput method on a fed-batch, rather than on a simple batch, process opens new perspectives for medium and feed development that enables identification of an optimized process in a short time frame. PMID:23563583

  4. Batching System for Superior Service

    NASA Technical Reports Server (NTRS)

    2001-01-01

    Veridian's Portable Batch System (PBS) was the recipient of the 1997 NASA Space Act Award for outstanding software. A batch system is a set of processes for managing queues and jobs. Without a batch system, it is difficult to manage the workload of a computer system. By bundling the enterprise's computing resources, the PBS technology offers users a single coherent interface, resulting in efficient management of the batch services. Users choose which information to package into "containers" for system-wide use. PBS also provides detailed system usage data, a procedure not easily executed without this software. PBS operates on networked, multi-platform UNIX environments. Veridian's new version, PBS Pro,TM has additional features and enhancements, including support for additional operating systems. Veridian distributes the original version of PBS as Open Source software via the PBS website. Customers can register and download the software at no cost. PBS Pro is also available via the web and offers additional features such as increased stability, reliability, and fault tolerance.A company using PBS can expect a significant increase in the effective management of its computing resources. Tangible benefits include increased utilization of costly resources and enhanced understanding of computational requirements and user needs.

  5. The Use of Lean Six Sigma Methodology in Increasing Capacity of a Chemical Production Facility at DSM.

    PubMed

    Meeuwse, Marco

    2018-03-30

    Lean Six Sigma is an improvement method, combining Lean, which focuses on removing 'waste' from a process, with Six Sigma, which is a data-driven approach, making use of statistical tools. Traditionally it is used to improve the quality of products (reducing defects), or processes (reducing variability). However, it can also be used as a tool to increase the productivity or capacity of a production plant. The Lean Six Sigma methodology is therefore an important pillar of continuous improvement within DSM. In the example shown here a multistep batch process is improved, by analyzing the duration of the relevant process steps, and optimizing the procedures. Process steps were performed in parallel instead of sequential, and some steps were made shorter. The variability was reduced, giving the opportunity to make a tighter planning, and thereby reducing waiting times. Without any investment in new equipment or technical modifications, the productivity of the plant was improved by more than 20%; only by changing procedures and the programming of the process control system.

  6. Kinetic modelling of starch and lipid formation during mixotrophic, nutrient-limited microalgal growth.

    PubMed

    Figueroa-Torres, Gonzalo M; Pittman, Jon K; Theodoropoulos, Constantinos

    2017-10-01

    Microalgal starch and lipids, carbon-based storage molecules, are useful as potential biofuel feedstocks. In this work, cultivation strategies maximising starch and lipid formation were established by developing a multi-parameter kinetic model describing microalgal growth as well as starch and lipid formation, in conjunction with laboratory-scale experiments. Growth dynamics are driven by nitrogen-limited mixotrophic conditions, known to increase cellular starch and lipid contents whilst enhancing biomass growth. Model parameters were computed by fitting model outputs to a range of experimental datasets from batch cultures of Chlamydomonas reinhardtii. Predictive capabilities of the model were established against different experimental data. The model was subsequently used to compute optimal nutrient-based cultivation strategies in terms of initial nitrogen and carbon concentrations. Model-based optimal strategies yielded a significant increase of 261% for starch (0.065gCL -1 ) and 66% for lipid (0.08gCL -1 ) production compared to base-case conditions (0.018gCL -1 starch, 0.048gCL -1 lipids). Copyright © 2017 Elsevier Ltd. All rights reserved.

  7. Establishment of replacement batches for heparin low-molecular-mass for calibration CRS, and the International Standard Low Molecular Weight Heparin for Calibration.

    PubMed

    Mulloy, B; Heath, A; Behr-Gross, M-E

    2007-12-01

    An international collaborative study involving fourteen laboratories has taken place, organised by the European Directorate for the Quality of Medicines & HealthCare (EDQM) with National Institute for Biological Standards & Control (NIBSC) (in its capacity as a World Health Organisation (WHO) Laboratory for Biological Standardisation) to provide supporting data for the establishment of replacement batches of Heparin Low-Molecular-Mass (LMM) for Calibration Chemical Reference Substance (CRS), and of the International Reference Reagent (IRR) Low Molecular Weight Heparin for Molecular Weight Calibration. A batch of low-molecular-mass heparin was donated to the organisers and candidate preparations of freeze-dried heparin were produced at NIBSC and EDQM. The establishment study was organised in two phases: a prequalification (phase 1, performed in 3 laboratories in 2005) followed by an international collaborative study (phase 2). In phase 2, started in March 2006, molecular mass parameters were determined for seven different LMM heparin samples using the current CRS batch and two batches of candidate replacement material with a defined number average relative molecular mass (Mn) of 3,700, determined in phase 1. The values calculated using the candidates as standard were systematically different from values calculated using the current batch with its assigned number-average molecular mass (Mna) of 3,700. Using raw data supplied by participants, molecular mass parameters were recalculated using the candidates as standard with values for Mna of 3,800 and 3,900. Values for these parameters agreed more closely with those calculated using the current batch supporting the fact that the candidates, though similar to batch 1 in view of the production processes used, differ slightly in terms of molecular mass distribution. Therefore establishment of the candidates was recommended with an assigned Mna value of 3,800 that is both consistent with phase 1 results and guarantees continuity with the current CRS batch. In phase 2, participants also determined molecular weight parameters for the seven different LMM heparin samples using both the 1st IRR (90/686) and its Broad Standard Table and the candidate World Health Organization (WHO) 2nd International Standard (05/112) (2nd IS) using a Broad Standard Table established in phase 1. Mean molecular weights calculated using 2nd IS were slightly higher than with 1st IRR, and participants in the study indicated that this systematic difference precluded establishment of 2nd IS with the table supplied. A replacement Broad Standard Table has been devised on the basis of the central recalculations of raw data supplied by participants; this table gives improved agreement between values derived using the 1st IRR and the candidate 2nd IS. On the basis of this study a recommendation was made for the establishment of 2nd IS and its proposed Broad Standard Table as a replacement for the 1st International Reference Reagent Low Molecular Weight Heparin for Molecular Weight Calibration. Unlike the 1st IRR however, the candidate material 2nd IS is not suitable for use with the method of Nielsen. The candidate materials were established as heparin low-molecular-mass for calibration batches 2 and 3 by the Ph. Eur. Commission in March 2007 and as 2nd IS low-molecular-weight heparin for molecular weight calibration (05/112) by the Expert Committee on Biological Standardization in November 2007.

  8. Designing a Dynamic Data Driven Application System for Estimating Real-Time Load of DOC in a River

    NASA Astrophysics Data System (ADS)

    Ouyang, Y.; None

    2011-12-01

    Understanding the dynamics of naturally occurring dissolved organic carbon (DOC) in a river is central to estimating surface water quality, aquatic carbon cycling, and climate change. Currently, determination of DOC in surface water is primarily accomplished by manually collecting samples for laboratory analysis, which requires at least 24 hours. In other words, no effort has been devoted to monitoring real-time variations of DOC in a river due to the lack of suitable and/or cost-effective wireless sensors. However, when considering human health, carbon footprints, and effects of urbanization, industry, and agriculture on water resource supply, timely DOC information may be critical. We have developed here a new paradigm, a dynamic data driven application system (DDDAS), for estimating the real-time load of DOC into a river. This DDDAS consisted of the following four components: (1) a Visual Basic (VB) program for downloading US Geological Survey real-time chlorophyll and discharge data; (2) a STELLA model for evaluating real-time DOC load based on the relationship between chlorophyll a, DOC, and river discharge; (3) a batch file for linking the VB program and STELLA model; and (4) a Microsoft Windows Scheduled Tasks wizard for executing the model and displaying output on a computer screen at selected times. Results show that the real-time load of DOC into the St. Johns River basin near Satsuma, Putnam County, Florida, USA varied over a range from -13,143 to 29,248 kg/h at the selected site in Florida, USA. The negative loads occurred because of the back flow in the estuarine reach of the river. The cumulative load of DOC in the river for the selected site at the end of the simulation (178 hours) was about 1.2 tons. Our results support the utility of the DDDAS developed in this study for estimating the real-time variations of DOC in river ecosystems.

  9. A universal piezo-driven ultrasonic cell microinjection system.

    PubMed

    Huang, Haibo; Mills, James K; Lu, Cong; Sun, Dong

    2011-08-01

    Over the past decade, the rapid development of biotechnologies such as gene injection, in-vitro fertilization, intracytoplasmic sperm injection (ICSI) and drug development have led to great demand for highly automated, high precision equipment for microinjection. Recently a new cell injection technology using piezo-driven pipettes with a very small mercury column was proposed and successfully applied in ICSI to a variety of mammal species. Although this technique significantly improves the survival rates of the ICSI process, shortcomings due to the toxicity of mercury and damage to the cell membrane due to large lateral tip oscillations of the injector pipette may limit its application. In this paper, a new cell injection system for automatic batch injection of suspended cells is developed. A new design of the piezo-driven cell injector is proposed for automated suspended cell injection. This new piezo-driven cell injector design relocates the piezo oscillation actuator to the injector pipette which eliminates the vibration effect on other parts of the micromanipulator. A small piezo stack is sufficient to perform the cell injection process. Harmful lateral tip oscillations of the injector pipette are reduced substantially without the use of a mercury column. Furthermore, ultrasonic vibration micro-dissection (UVM) theory is utilized to analyze the piezo-driven cell injection process, and the source of the lateral oscillations of the injector pipette is investigated. From preliminary experiments of cell injection of a large number of zebrafish embryos (n = 200), the injector pipette can easily pierce through the cell membrane at a low injection speed and almost no deformation of the cell wall, and with a high success rate(96%) and survival rate(80.7%) This new injection approach shows good potential for precision injection with less damage to the injected cells.

  10. A feasibility study on biological nitrogen removal (BNR) via integrated thiosulfate-driven denitratation with anammox.

    PubMed

    Qian, Jin; Zhang, Mingkuan; Wu, Yaoguo; Niu, Juntao; Chang, Xing; Yao, Hairui; Hu, Sihai; Pei, Xiangjun

    2018-06-12

    To exploit the advantages of less electron donor consumptions in partial-denitrification (denitratation, NO 3 - → NO 2 - ) as well as less sludge production in autotrophic denitrification (AD) and anammox, a novel biological nitrogen removal (BNR) process through combined anammox and thiosulfate-driven denitratation was proposed here. In this study, the ratio of S 2 O 3 2- -S/NO 3 - -N and pH are confirmed to be two key factors affecting the thiosulfate-driven denitratation activity and nitrite accumulation. Simultaneous high denitratation activity and substantial nitrite accumulation were observed at initial S 2 O 3 2- -S/NO 3 - -N ratio of 1.5:1 and pH of 8.0. The optimal pH for the anammox reaction is determined to be 8.0. A sequential batch reactor (SBR) and an up-flow anaerobic sludge blanket (UASB) reactor were established to proceed the anammox and the high-rate thiosulfate-driven denitratation, respectively. Under the ambient temperature of 35 °C, the total nitrogen removal efficiency and capacity are 73% and 0.35 kg N/day/m 3 in the anammox-SBR. At HRT of 30 min, the NO 3 - removal efficiency could achieve above 90% with the nitrate-to-nitrite transformation ratio of 0.8, implying the great potential to apply the thiosulfate-driven denitratation & anammox system for BNR with minimal sludge production. Without the occurrence of denitritation (NO 2 - → N 2 O → N 2 ), theoretically no N 2 O could be emitted from this BNR system. This study could shed light on how to operate a high rate BNR system targeting to electron donor and energy savings as well as biowastes minimization and greenhouse gas reductions. Copyright © 2018. Published by Elsevier Ltd.

  11. TDRSS-user orbit determination using batch least-squares and sequential methods

    NASA Astrophysics Data System (ADS)

    Oza, D. H.; Jones, T. L.; Hakimi, M.; Samii, Mina V.; Doll, C. E.; Mistretta, G. D.; Hart, R. C.

    1993-02-01

    The Goddard Space Flight Center (GSFC) Flight Dynamics Division (FDD) commissioned Applied Technology Associates, Incorporated, to develop the Real-Time Orbit Determination/Enhanced (RTOD/E) system on a Disk Operating System (DOS)-based personal computer (PC) as a prototype system for sequential orbit determination of spacecraft. This paper presents the results of a study to compare the orbit determination accuracy for a Tracking and Data Relay Satellite System (TDRSS) user spacecraft, Landsat-4, obtained using RTOD/E, operating on a PC, with the accuracy of an established batch least-squares system, the Goddard Trajectory Determination System (GTDS), and operating on a mainframe computer. The results of Landsat-4 orbit determination will provide useful experience for the Earth Observing System (EOS) series of satellites. The Landsat-4 ephemerides were estimated for the January 17-23, 1991, timeframe, during which intensive TDRSS tracking data for Landsat-4 were available. Independent assessments were made of the consistencies (overlap comparisons for the batch case and covariances and the first measurement residuals for the sequential case) of solutions produced by the batch and sequential methods. The forward-filtered RTOD/E orbit solutions were compared with the definitive GTDS orbit solutions for Landsat-4; the solution differences were less than 40 meters after the filter had reached steady state.

  12. Low-Rank Matrix Recovery Approach for Clutter Rejection in Real-Time IR-UWB Radar-Based Moving Target Detection

    PubMed Central

    Sabushimike, Donatien; Na, Seung You; Kim, Jin Young; Bui, Ngoc Nam; Seo, Kyung Sik; Kim, Gil Gyeom

    2016-01-01

    The detection of a moving target using an IR-UWB Radar involves the core task of separating the waves reflected by the static background and by the moving target. This paper investigates the capacity of the low-rank and sparse matrix decomposition approach to separate the background and the foreground in the trend of UWB Radar-based moving target detection. Robust PCA models are criticized for being batched-data-oriented, which makes them inconvenient in realistic environments where frames need to be processed as they are recorded in real time. In this paper, a novel method based on overlapping-windows processing is proposed to cope with online processing. The method consists of processing a small batch of frames which will be continually updated without changing its size as new frames are captured. We prove that RPCA (via its Inexact Augmented Lagrange Multiplier (IALM) model) can successfully separate the two subspaces, which enhances the accuracy of target detection. The overlapping-windows processing method converges on the optimal solution with its batch counterpart (i.e., processing batched data with RPCA), and both methods prove the robustness and efficiency of the RPCA over the classic PCA and the commonly used exponential averaging method. PMID:27598159

  13. Comparison of ERBS orbit determination accuracy using batch least-squares and sequential methods

    NASA Technical Reports Server (NTRS)

    Oza, D. H.; Jones, T. L.; Fabien, S. M.; Mistretta, G. D.; Hart, R. C.; Doll, C. E.

    1991-01-01

    The Flight Dynamics Div. (FDD) at NASA-Goddard commissioned a study to develop the Real Time Orbit Determination/Enhanced (RTOD/E) system as a prototype system for sequential orbit determination of spacecraft on a DOS based personal computer (PC). An overview is presented of RTOD/E capabilities and the results are presented of a study to compare the orbit determination accuracy for a Tracking and Data Relay Satellite System (TDRSS) user spacecraft obtained using RTOS/E on a PC with the accuracy of an established batch least squares system, the Goddard Trajectory Determination System (GTDS), operating on a mainframe computer. RTOD/E was used to perform sequential orbit determination for the Earth Radiation Budget Satellite (ERBS), and the Goddard Trajectory Determination System (GTDS) was used to perform the batch least squares orbit determination. The estimated ERBS ephemerides were obtained for the Aug. 16 to 22, 1989, timeframe, during which intensive TDRSS tracking data for ERBS were available. Independent assessments were made to examine the consistencies of results obtained by the batch and sequential methods. Comparisons were made between the forward filtered RTOD/E orbit solutions and definitive GTDS orbit solutions for ERBS; the solution differences were less than 40 meters after the filter had reached steady state.

  14. TDRSS-user orbit determination using batch least-squares and sequential methods

    NASA Technical Reports Server (NTRS)

    Oza, D. H.; Jones, T. L.; Hakimi, M.; Samii, Mina V.; Doll, C. E.; Mistretta, G. D.; Hart, R. C.

    1993-01-01

    The Goddard Space Flight Center (GSFC) Flight Dynamics Division (FDD) commissioned Applied Technology Associates, Incorporated, to develop the Real-Time Orbit Determination/Enhanced (RTOD/E) system on a Disk Operating System (DOS)-based personal computer (PC) as a prototype system for sequential orbit determination of spacecraft. This paper presents the results of a study to compare the orbit determination accuracy for a Tracking and Data Relay Satellite System (TDRSS) user spacecraft, Landsat-4, obtained using RTOD/E, operating on a PC, with the accuracy of an established batch least-squares system, the Goddard Trajectory Determination System (GTDS), and operating on a mainframe computer. The results of Landsat-4 orbit determination will provide useful experience for the Earth Observing System (EOS) series of satellites. The Landsat-4 ephemerides were estimated for the January 17-23, 1991, timeframe, during which intensive TDRSS tracking data for Landsat-4 were available. Independent assessments were made of the consistencies (overlap comparisons for the batch case and covariances and the first measurement residuals for the sequential case) of solutions produced by the batch and sequential methods. The forward-filtered RTOD/E orbit solutions were compared with the definitive GTDS orbit solutions for Landsat-4; the solution differences were less than 40 meters after the filter had reached steady state.

  15. Comparison of ERBS orbit determination accuracy using batch least-squares and sequential methods

    NASA Astrophysics Data System (ADS)

    Oza, D. H.; Jones, T. L.; Fabien, S. M.; Mistretta, G. D.; Hart, R. C.; Doll, C. E.

    1991-10-01

    The Flight Dynamics Div. (FDD) at NASA-Goddard commissioned a study to develop the Real Time Orbit Determination/Enhanced (RTOD/E) system as a prototype system for sequential orbit determination of spacecraft on a DOS based personal computer (PC). An overview is presented of RTOD/E capabilities and the results are presented of a study to compare the orbit determination accuracy for a Tracking and Data Relay Satellite System (TDRSS) user spacecraft obtained using RTOS/E on a PC with the accuracy of an established batch least squares system, the Goddard Trajectory Determination System (GTDS), operating on a mainframe computer. RTOD/E was used to perform sequential orbit determination for the Earth Radiation Budget Satellite (ERBS), and the Goddard Trajectory Determination System (GTDS) was used to perform the batch least squares orbit determination. The estimated ERBS ephemerides were obtained for the Aug. 16 to 22, 1989, timeframe, during which intensive TDRSS tracking data for ERBS were available. Independent assessments were made to examine the consistencies of results obtained by the batch and sequential methods. Comparisons were made between the forward filtered RTOD/E orbit solutions and definitive GTDS orbit solutions for ERBS; the solution differences were less than 40 meters after the filter had reached steady state.

  16. Online Bagging and Boosting

    NASA Technical Reports Server (NTRS)

    Oza, Nikunji C.

    2005-01-01

    Bagging and boosting are two of the most well-known ensemble learning methods due to their theoretical performance guarantees and strong experimental results. However, these algorithms have been used mainly in batch mode, i.e., they require the entire training set to be available at once and, in some cases, require random access to the data. In this paper, we present online versions of bagging and boosting that require only one pass through the training data. We build on previously presented work by presenting some theoretical results. We also compare the online and batch algorithms experimentally in terms of accuracy and running time.

  17. Cocoa residues as viable biomass for renewable energy production through anaerobic digestion.

    PubMed

    Acosta, Nayaret; De Vrieze, Jo; Sandoval, Verónica; Sinche, Danny; Wierinck, Isabella; Rabaey, Korneel

    2018-05-31

    The aim of this work was to evaluate the bioenergy potential of cocoa residue via anaerobic digestion. Batch and fed-batch lab-scale reactors were operated under low and high solids conditions. In the batch tests, 59 ± 4% of Chemical Oxygen Demand (COD) was recovered as methane. This corresponded with an average methane yield of 174 (wet) and 193 (dry) L kg -1 volatile solids fed, whereas a series of fed-batch reactors produced 70 ± 24 (wet) and 107 ± 39 (dry) L CH 4  kg -1 volatile solids fed during stable conditions. A case study was developed for canton Balao (Ecuador) based on our experimental data, operational estimates and available cocoa waste in the area. Annually, 8341 MWh could be produced, meeting 88% of the current electricity demand in Balao. This case study proves the potential for cocoa waste as a source of renewable energy in rural areas. Copyright © 2018 Elsevier Ltd. All rights reserved.

  18. An adsorption diffusion model for removal of para-chlorophenol by activated carbon derived from bituminous coal.

    PubMed

    Sze, M F F; McKay, G

    2010-05-01

    Batch adsorption experiments were carried out to study the adsorptive removal and diffusion mechanism of para-chlorophenol (p-CP) onto Calgon Filtrasorb 400 (F400) activated carbon. The external mass transfer resistance is negligible in the adsorption process carried out under different conditions in batch operation. Intraparticle diffusion model plots were used to correlate the batch p-CP adsorption data; three distinct linear sections were obtained for every batch operation. The textural properties of F400 activated carbon showed that it has a large portion of supermicropores, which is comparable to the size of the p-CP molecules. Due to the stronger interactions between p-CP molecules and F400 micropores, p-CP molecules predominantly diffused and occupied active sites in micropore region by hopping mechanism, and eventually followed by a slow filling of mesopores and micropores. This hypothesis is proven by the excellent agreement of the intraparticle diffusion model plots and the textural properties of F400 activated carbon. Copyright 2009 Elsevier Ltd. All rights reserved.

  19. Density separation as a strategy to reduce the enzyme load of preharvest sprouted wheat and enhance its bread making quality.

    PubMed

    Olaerts, Heleen; De Bondt, Yamina; Courtin, Christophe M

    2018-02-15

    As preharvest sprouting of wheat impairs its use in food applications, postharvest solutions for this problem are required. Due to the high kernel to kernel variability in enzyme activity in a batch of sprouted wheat, the potential of eliminating severely sprouted kernels based on density differences in NaCl solutions was evaluated. Compared to higher density kernels, lower density kernels displayed higher α-amylase, endoxylanase, and peptidase activities as well as signs of (incipient) protein, β-glucan and arabinoxylan breakdown. By discarding lower density kernels of mildly and severely sprouted wheat batches (11% and 16%, respectively), density separation increased flour FN of the batch from 280 to 345s and from 135 to 170s and increased RVA viscosity. This in turn improved dough handling, bread crumb texture and crust color. These data indicate that density separation is a powerful technique to increase the quality of a batch of sprouted wheat. Copyright © 2017 Elsevier Ltd. All rights reserved.

  20. Comfort for Sportsmen

    NASA Technical Reports Server (NTRS)

    1976-01-01

    MPI Outdoor Safety Products developed aluminized mylar to make Echo Satellites more reflective, to insulate cryogenic fluids, and for space suit insulation. This technology has spun off to a variety of consumer products. Sportsman's blankets and jackets, ski parkas, sleeping bags, and even life-raft canopies are among them. Sportsman's blanket weighing 12 ounces can be used equally well to keep heat away or keep available heat in. Emergency rescue blanket has heat retention qualities similar to those of Sportsman's blanket. Strong enough to be used as a litter, yet folds up so small you can carry it in your shirt pocket. 10 ounce reversible jacket absorbs warmth from sun. A silver colored side next to your body retains a large portion of body heat. In warm weather you wear silver side out to reflect sun's rays.

  1. Social Media's Initial Reaction to Information and Misinformation on Ebola, August 2014: Facts and Rumors.

    PubMed

    Fung, Isaac Chun-Hai; Fu, King-Wa; Chan, Chung-Hong; Chan, Benedict Shing Bun; Cheung, Chi-Ngai; Abraham, Thomas; Tse, Zion Tsz Ho

    2016-01-01

    We analyzed misinformation about Ebola circulating on Twitter and Sina Weibo, the leading Chinese microblog platform, at the outset of the global response to the 2014-2015 Ebola epidemic to help public health agencies develop their social media communication strategies. We retrieved Twitter and Sina Weibo data created within 24 hours of the World Health Organization announcement of a Public Health Emergency of International Concern (Batch 1 from August 8, 2014, 06:50:00 Greenwich Mean Time [GMT] to August 9, 2014, 06:49:59 GMT) and seven days later (Batch 2 from August 15, 2014, 06:50:00 GMT to August 16, 2014, 06:49:59 GMT). We obtained and analyzed a 1% random sample of tweets containing the keyword Ebola. We retrieved all Sina Weibo posts with Chinese keywords for Ebola for analysis. We analyzed changes in frequencies of keywords, hashtags, and Web links using relative risk (RR) and c(2) feature selection algorithm. We identified misinformation by manual coding and categorizing randomly selected sub-datasets. We identified two speculative treatments (i.e., bathing in or drinking saltwater and ingestion of Nano Silver, an experimental drug) in our analysis of changes in frequencies of keywords and hashtags. Saltwater was speculated to be protective against Ebola in Batch 1 tweets but their mentions decreased in Batch 2 (RR=0.11 for "salt" and RR=0.14 for "water"). Nano Silver mentions were higher in Batch 2 than in Batch 1 (RR=10.5). In our manually coded samples, Ebola-related misinformation constituted about 2% of Twitter and Sina Weibo content. A range of 36%-58% of the posts were news about the Ebola outbreak and 19%-24% of the posts were health information and responses to misinformation in both batches. In Batch 2, 43% of Chinese microblogs focused on the Chinese government sending medical assistance to Guinea. Misinformation about Ebola was circulated at a very low level globally in social media in either batch. Qualitative and quantitative analyses of social media posts can provide relevant information to public health agencies during emergency responses.

  2. Stratified randomization controls better for batch effects in 450K methylation analysis: a cautionary tale.

    PubMed

    Buhule, Olive D; Minster, Ryan L; Hawley, Nicola L; Medvedovic, Mario; Sun, Guangyun; Viali, Satupaitea; Deka, Ranjan; McGarvey, Stephen T; Weeks, Daniel E

    2014-01-01

    Batch effects in DNA methylation microarray experiments can lead to spurious results if not properly handled during the plating of samples. Two pilot studies examining the association of DNA methylation patterns across the genome with obesity in Samoan men were investigated for chip- and row-specific batch effects. For each study, the DNA of 46 obese men and 46 lean men were assayed using Illumina's Infinium HumanMethylation450 BeadChip. In the first study (Sample One), samples from obese and lean subjects were examined on separate chips. In the second study (Sample Two), the samples were balanced on the chips by lean/obese status, age group, and census region. We used methylumi, watermelon, and limma R packages, as well as ComBat, to analyze the data. Principal component analysis and linear regression were, respectively, employed to identify the top principal components and to test for their association with the batches and lean/obese status. To identify differentially methylated positions (DMPs) between obese and lean males at each locus, we used a moderated t-test. Chip effects were effectively removed from Sample Two but not Sample One. In addition, dramatic differences were observed between the two sets of DMP results. After "removing" batch effects with ComBat, Sample One had 94,191 probes differentially methylated at a q-value threshold of 0.05 while Sample Two had zero differentially methylated probes. The disparate results from Sample One and Sample Two likely arise due to the confounding of lean/obese status with chip and row batch effects. Even the best possible statistical adjustments for batch effects may not completely remove them. Proper study design is vital for guarding against spurious findings due to such effects.

  3. Stratified randomization controls better for batch effects in 450K methylation analysis: a cautionary tale

    PubMed Central

    Buhule, Olive D.; Minster, Ryan L.; Hawley, Nicola L.; Medvedovic, Mario; Sun, Guangyun; Viali, Satupaitea; Deka, Ranjan; McGarvey, Stephen T.; Weeks, Daniel E.

    2014-01-01

    Background: Batch effects in DNA methylation microarray experiments can lead to spurious results if not properly handled during the plating of samples. Methods: Two pilot studies examining the association of DNA methylation patterns across the genome with obesity in Samoan men were investigated for chip- and row-specific batch effects. For each study, the DNA of 46 obese men and 46 lean men were assayed using Illumina's Infinium HumanMethylation450 BeadChip. In the first study (Sample One), samples from obese and lean subjects were examined on separate chips. In the second study (Sample Two), the samples were balanced on the chips by lean/obese status, age group, and census region. We used methylumi, watermelon, and limma R packages, as well as ComBat, to analyze the data. Principal component analysis and linear regression were, respectively, employed to identify the top principal components and to test for their association with the batches and lean/obese status. To identify differentially methylated positions (DMPs) between obese and lean males at each locus, we used a moderated t-test. Results: Chip effects were effectively removed from Sample Two but not Sample One. In addition, dramatic differences were observed between the two sets of DMP results. After “removing” batch effects with ComBat, Sample One had 94,191 probes differentially methylated at a q-value threshold of 0.05 while Sample Two had zero differentially methylated probes. The disparate results from Sample One and Sample Two likely arise due to the confounding of lean/obese status with chip and row batch effects. Conclusion: Even the best possible statistical adjustments for batch effects may not completely remove them. Proper study design is vital for guarding against spurious findings due to such effects. PMID:25352862

  4. Transfer of a three step mAb chromatography process from batch to continuous: Optimizing productivity to minimize consumable requirements.

    PubMed

    Gjoka, Xhorxhi; Gantier, Rene; Schofield, Mark

    2017-01-20

    The goal of this study was to adapt a batch mAb purification chromatography platform for continuous operation. The experiments and rationale used to convert from batch to continuous operation are described. Experimental data was used to design chromatography methods for continuous operation that would exceed the threshold for critical quality attributes and minimize the consumables required as compared to batch mode of operation. Four unit operations comprising of Protein A capture, viral inactivation, flow-through anion exchange (AEX), and mixed-mode cation exchange chromatography (MMCEX) were integrated across two Cadence BioSMB PD multi-column chromatography systems in order to process a 25L volume of harvested cell culture fluid (HCCF) in less than 12h. Transfer from batch to continuous resulted in an increase in productivity of the Protein A step from 13 to 50g/L/h and of the MMCEX step from 10 to 60g/L/h with no impact on the purification process performance in term of contaminant removal (4.5 log reduction of host cell proteins, 50% reduction in soluble product aggregates) and overall chromatography process yield of recovery (75%). The increase in productivity, combined with continuous operation, reduced the resin volume required for Protein A and MMCEX chromatography by more than 95% compared to batch. The volume of AEX membrane required for flow through operation was reduced by 74%. Moreover, the continuous process required 44% less buffer than an equivalent batch process. This significant reduction in consumables enables cost-effective, disposable, single-use manufacturing. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  5. Modeling Lab-sized Anaerobic Fluidized Bed Reactor (AFBR) for Palm Oil Mill Effluent (POME) treatment: from Batch to Continuous Reactors

    NASA Astrophysics Data System (ADS)

    Mufti Azis, Muhammad; Sudibyo, Hanifrahmawan; Budhijanto, Wiratni

    2018-03-01

    Indonesia is aiming to produce 30 million tones/year of crude palm oil (CPO) by 2020. As a result, 90 million tones/year of POME will be produced. POME is highly polluting wastewater which may cause severe environmental problem due to its high chemical oxygen demand (COD) and biochemical oxygen demand (BOD). Due to the limitation of open pond treatment, the use of AFBR has been considered as a potential technology to treat POME. This study aims to develop mathematical models of lab-sized Anaerobic Fluidized Bed Reactor (AFBR) in batch and continuous processes. In addition, the AFBR also utilized natural zeolite as an immobilized media for microbes. To initiate the biomass growth, biodiesel waste has been used as an inoculum. In the first part of this study, a batch AFBR was operated to evaluate the COD, VFA, and CH4 concentrations. By comparing the batch results with and without zeolite, it showed that the addition of 17 g/gSCOD zeolite gave larger COD decrease within 20 days of operation. In order to elucidate the mechanism, parameter estimations of 12 kinetic parameters were proposed to describe the batch reactor performance. The model in general could describe the batch experimental data well. In the second part of this study, the kinetic parameters obtained from batch reactor were used to simulate the performance of double column AFBR where the acidogenic and methanogenic biomass were separated. The simulation showed that a relatively long residence time (Hydraulic Residence Time, HRT) was required to treat POME using the proposed double column AFBR. Sensitivity analyses was conducted and revealed that μm1 appeared to be the most sensitive parameter to reduce the HRT of double column AFBR.

  6. Improved solution accuracy for TDRSS-based TOPEX/Poseidon orbit determination

    NASA Technical Reports Server (NTRS)

    Doll, C. E.; Mistretta, G. D.; Hart, R. C.; Oza, D. H.; Bolvin, D. T.; Cox, C. M.; Nemesure, M.; Niklewski, D. J.; Samii, M. V.

    1994-01-01

    Orbit determination results are obtained by the Goddard Space Flight Center (GSFC) Flight Dynamics Division (FDD) using a batch-least-squares estimator available in the Goddard Trajectory Determination System (GTDS) and an extended Kalman filter estimation system to process Tracking and Data Relay Satellite (TDRS) System (TDRSS) measurements. GTDS is the operational orbit determination system used by the FDD in support of the Ocean Topography Experiment (TOPEX)/Poseidon spacecraft navigation and health and safety operations. The extended Kalman filter was implemented in an orbit determination analysis prototype system, closely related to the Real-Time Orbit Determination System/Enhanced (RTOD/E) system. In addition, the Precision Orbit Determination (POD) team within the GSFC Space Geodesy Branch generated an independent set of high-accuracy trajectories to support the TOPEX/Poseidon scientific data. These latter solutions use the geodynamics (GEODYN) orbit determination system with laser ranging and Doppler Orbitography and Radiopositioning integrated by satellite (DORIS) tracking measurements. The TOPEX/Poseidon trajectories were estimated for November 7 through November 11, 1992, the timeframe under study. Independent assessments were made of the consistencies of solutions produced by the batch and sequential methods. The batch-least-squares solutions were assessed based on the solution residuals, while the sequential solutions were assessed based on primarily the estimated covariances. The batch-least-squares and sequential orbit solutions were compared with the definitive POD orbit solutions. The solution differences were generally less than 2 meters for the batch-least-squares and less than 13 meters for the sequential estimation solutions. After the sequential estimation solutions were processed with a smoother algorithm, position differences with POD orbit solutions of less than 7 meters were obtained. The differences among the POD, GTDS, and filter/smoother solutions can be traced to differences in modeling and tracking data types, which are being analyzed in detail.

  7. The Long Non-Coding RNA Transcriptome Landscape in CHO Cells Under Batch and Fed-Batch Conditions.

    PubMed

    Vito, Davide; Smales, C Mark

    2018-05-21

    The role of non-coding RNAs in determining growth, productivity and recombinant product quality attributes in Chinese hamster ovary (CHO) cells has received much attention in recent years, exemplified by studies into microRNAs in particular. However, other classes of non-coding RNAs have received less attention. One such class are the non-coding RNAs known collectively as long non-coding RNAs (lncRNAs). We have undertaken the first landscape analysis of the lncRNA transcriptome in CHO using a mouse based microarray that also provided for the surveillance of the coding transcriptome. We report on those lncRNAs present in a model host CHO cell line under batch and fed-batch conditions on two different days and relate the expression of different lncRNAs to each other. We demonstrate that the mouse microarray was suitable for the detection and analysis of thousands of CHO lncRNAs and validated a number of these by qRT-PCR. We then further analysed the data to identify those lncRNAs whose expression changed the most between growth and stationary phases of culture or between batch and fed-batch culture to identify potential lncRNA targets for further functional studies with regard to their role in controlling growth of CHO cells. We discuss the implications for the publication of this rich dataset and how this may be used by the community. This article is protected by copyright. All rights reserved.

  8. Impact of Nisin-Activated Packaging on Microbiota of Beef Burgers during Storage

    PubMed Central

    Ferrocino, Ilario; Greppi, Anna; La Storia, Antonietta; Rantsiou, Kalliopi; Ercolini, Danilo

    2015-01-01

    Beef burgers were stored at 4°C in a vacuum in nisin-activated antimicrobial packaging. Microbial ecology analyses were performed on samples collected between days 0 and 21 of storage to discover the population diversity. Two batches were analyzed using RNA-based denaturing gradient gel electrophoresis (DGGE) and pyrosequencing. The active packaging retarded the growth of the total viable bacteria and lactic acid bacteria. Culture-independent analysis by pyrosequencing of RNA extracted directly from meat showed that Photobacterium phosphoreum, Lactococcus piscium, Lactobacillus sakei, and Leuconostoc carnosum were the major operational taxonomic units (OTUs) shared between control and treated samples. Beta diversity analysis of the 16S rRNA sequence data and RNA-DGGE showed a clear separation between two batches based on the microbiota. Control samples from batch B showed a significant high abundance of some taxa sensitive to nisin, such as Kocuria rhizophila, Staphylococcus xylosus, Leuconostoc carnosum, and Carnobacterium divergens, compared to control samples from batch A. However, only from batch B was it possible to find a significant difference between controls and treated samples during storage due to the active packaging. Predicted metagenomes confirmed differences between the two batches and indicated that the use of nisin-based antimicrobial packaging can determine a reduction in the abundance of specific metabolic pathways related to spoilage. The present study aimed to assess the viable bacterial communities in beef burgers stored in nisin-based antimicrobial packaging, and it highlights the efficacy of this strategy to prolong beef burger shelf life. PMID:26546424

  9. Army Synthetic Validity Project. Report of Phase 3 Results. Volume 1.

    DTIC Science & Technology

    1991-02-01

    the " new " jobs . The "existing" jobs...were the Batch A MOS for which we had the more comprehensive data sets and, generally speaking, larger samples. The " new " jobs were the nine Batch Z...4.2). Second, we applied the empirical least squares equation for the "existing" job that most closely matched each "new" job to the " new " jobs

  10. Heterotrophs are key contributors to nitrous oxide production in activated sludge under low C-to-N ratios during nitrification-Batch experiments and modeling.

    PubMed

    Domingo-Félez, Carlos; Pellicer-Nàcher, Carles; Petersen, Morten S; Jensen, Marlene M; Plósz, Benedek G; Smets, Barth F

    2017-01-01

    Nitrous oxide (N 2 O), a by-product of biological nitrogen removal during wastewater treatment, is produced by ammonia-oxidizing bacteria (AOB) and heterotrophic denitrifying bacteria (HB). Mathematical models are used to predict N 2 O emissions, often including AOB as the main N 2 O producer. Several model structures have been proposed without consensus calibration procedures. Here, we present a new experimental design that was used to calibrate AOB-driven N 2 O dynamics of a mixed culture. Even though AOB activity was favoured with respect to HB, oxygen uptake rates indicated HB activity. Hence, rigorous experimental design for calibration of autotrophic N 2 O production from mixed cultures is essential. The proposed N 2 O production pathways were examined using five alternative process models confronted with experimental data inferred. Individually, the autotrophic and heterotrophic denitrification pathway could describe the observed data. In the best-fit model, which combined two denitrification pathways, the heterotrophic was stronger than the autotrophic contribution to N 2 O production. Importantly, the individual contribution of autotrophic and heterotrophic to the total N 2 O pool could not be unambiguously elucidated solely based on bulk N 2 O measurements. Data on NO would increase the practical identifiability of N 2 O production pathways. Biotechnol. Bioeng. 2017;114: 132-140. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  11. Recommendation of ruthenium source for sludge batch flowsheet studies

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Woodham, W.

    Included herein is a preliminary analysis of previously-generated data from sludge batches 7a, 7b, 8, and 9 sludge simulant and real-waste testing, performed to recommend a form of ruthenium for future sludge batch simulant testing under the nitric-formic flowsheet. Focus is given to reactions present in the Sludge Receipt and Adjustment Tank cycle, given that this cycle historically produces the most changes in chemical composition during Chemical Process Cell processing. Data is presented and analyzed for several runs performed under the nitric-formic flowsheet, with consideration given to effects on the production of hydrogen gas, nitrous oxide gas, consumption of formate,more » conversion of nitrite to nitrate, and the removal and recovery of mercury during processing. Additionally, a brief discussion is given to the effect of ruthenium source selection under the nitric-glycolic flowsheet. An analysis of data generated from scaled demonstration testing, sludge batch 9 qualification testing, and antifoam degradation testing under the nitric-glycolic flowsheet is presented. Experimental parameters of interest under the nitric-glycolic flowsheet include N2O production, glycolate destruction, conversion of glycolate to formate and oxalate, and the conversion of nitrite to nitrate. To date, the number of real-waste experiments that have been performed under the nitric-glycolic flowsheet is insufficient to provide a complete understanding of the effects of ruthenium source selection in simulant experiments with regard to fidelity to real-waste testing. Therefore, a determination of comparability between the two ruthenium sources as employed under the nitric-glycolic flowsheet is made based on available data in order to inform ruthenium source selection for future testing under the nitric-glycolic flowsheet.« less

  12. Comparison of TOPEX/Poseidon orbit determination solutions obtained by the Goddard Space Flight Center Flight Dynamics Division and Precision Orbit Determination Teams

    NASA Technical Reports Server (NTRS)

    Doll, C.; Mistretta, G.; Hart, R.; Oza, D.; Cox, C.; Nemesure, M.; Bolvin, D.; Samii, Mina V.

    1993-01-01

    Orbit determination results are obtained by the Goddard Space Flight Center (GSFC) Flight Dynamics Division (FDD) using the Goddard Trajectory Determination System (GTDS) and a real-time extended Kalman filter estimation system to process Tracking Data and Relay Satellite (TDRS) System (TDRSS) measurements in support of the Ocean Topography Experiment (TOPEX)/Poseidon spacecraft navigation and health and safety operations. GTDS is the operational orbit determination system used by the FDD, and the extended Kalman fliter was implemented in an analysis prototype system, the Real-Time Orbit Determination System/Enhanced (RTOD/E). The Precision Orbit Determination (POD) team within the GSFC Space Geodesy Branch generates an independent set of high-accuracy trajectories to support the TOPEX/Poseidon scientific data. These latter solutions use the Geodynamics (GEODYN) orbit determination system with laser ranging tracking data. The TOPEX/Poseidon trajectories were estimated for the October 22 - November 1, 1992, timeframe, for which the latest preliminary POD results were available. Independent assessments were made of the consistencies of solutions produced by the batch and sequential methods. The batch cases were assessed using overlap comparisons, while the sequential cases were assessed with covariances and the first measurement residuals. The batch least-squares and forward-filtered RTOD/E orbit solutions were compared with the definitive POD orbit solutions. The solution differences were generally less than 10 meters (m) for the batch least squares and less than 18 m for the sequential estimation solutions. The differences among the POD, GTDS, and RTOD/E solutions can be traced to differences in modeling and tracking data types, which are being analyzed in detail.

  13. Comparative study of disinfectants for use in low-cost gravity driven household water purifiers.

    PubMed

    Patil, Rajshree A; Kausley, Shankar B; Balkunde, Pradeep L; Malhotra, Chetan P

    2013-09-01

    Point-of-use (POU) gravity-driven household water purifiers have been proven to be a simple, low-cost and effective intervention for reducing the impact of waterborne diseases in developing countries. The goal of this study was to compare commonly used water disinfectants for their feasibility of adoption in low-cost POU water purifiers. The potency of each candidate disinfectant was evaluated by conducting a batch disinfection study for estimating the concentration of disinfectant needed to inactivate a given concentration of the bacterial strain Escherichia coli ATCC 11229. Based on the concentration of disinfectant required, the size, weight and cost of a model purifier employing that disinfectant were estimated. Model purifiers based on different disinfectants were compared and disinfectants which resulted in the most safe, compact and inexpensive purifiers were identified. Purifiers based on bromine, tincture iodine, calcium hypochlorite and sodium dichloroisocyanurate were found to be most efficient, cost effective and compact with replacement parts costing US$3.60-6.00 for every 3,000 L of water purified and are thus expected to present the most attractive value proposition to end users.

  14. Estimating Animal Abundance in Ground Beef Batches Assayed with Molecular Markers

    PubMed Central

    Hu, Xin-Sheng; Simila, Janika; Platz, Sindey Schueler; Moore, Stephen S.; Plastow, Graham; Meghen, Ciaran N.

    2012-01-01

    Estimating animal abundance in industrial scale batches of ground meat is important for mapping meat products through the manufacturing process and for effectively tracing the finished product during a food safety recall. The processing of ground beef involves a potentially large number of animals from diverse sources in a single product batch, which produces a high heterogeneity in capture probability. In order to estimate animal abundance through DNA profiling of ground beef constituents, two parameter-based statistical models were developed for incidence data. Simulations were applied to evaluate the maximum likelihood estimate (MLE) of a joint likelihood function from multiple surveys, showing superiority in the presence of high capture heterogeneity with small sample sizes, or comparable estimation in the presence of low capture heterogeneity with a large sample size when compared to other existing models. Our model employs the full information on the pattern of the capture-recapture frequencies from multiple samples. We applied the proposed models to estimate animal abundance in six manufacturing beef batches, genotyped using 30 single nucleotide polymorphism (SNP) markers, from a large scale beef grinding facility. Results show that between 411∼1367 animals were present in six manufacturing beef batches. These estimates are informative as a reference for improving recall processes and tracing finished meat products back to source. PMID:22479559

  15. Kinetic studies on batch cultivation of Trichoderma reesei and application to enhance cellulase production by fed-batch fermentation.

    PubMed

    Ma, Lijuan; Li, Chen; Yang, Zhenhua; Jia, Wendi; Zhang, Dongyuan; Chen, Shulin

    2013-07-20

    Reducing the production cost of cellulase as the key enzyme for cellulose hydrolysis to fermentable sugars remains a major challenge for biofuel production. Because of the complexity of cellulase production, kinetic modeling and mass balance calculation can be used as effective tools for process design and optimization. In this study, kinetic models for cell growth, substrate consumption and cellulase production in batch fermentation were developed, and then applied in fed-batch fermentation to enhance cellulase production. Inhibition effect of substrate was considered and a modified Luedeking-Piret model was developed for cellulase production and substrate consumption according to the growth characteristics of Trichoderma reesei. The model predictions fit well with the experimental data. Simulation results showed that higher initial substrate concentration led to decrease of cellulase production rate. Mass balance and kinetic simulation results were applied to determine the feeding strategy. Cellulase production and its corresponding productivity increased by 82.13% after employing the proper feeding strategy in fed-batch fermentation. This method combining mathematics and chemometrics by kinetic modeling and mass balance can not only improve cellulase fermentation process, but also help to better understand the cellulase fermentation process. The model development can also provide insight to other similar fermentation processes. Copyright © 2013 Elsevier B.V. All rights reserved.

  16. Image data-processing system for solar astronomy

    NASA Technical Reports Server (NTRS)

    Wilson, R. M.; Teuber, D. L.; Watkins, J. R.; Thomas, D. T.; Cooper, C. M.

    1977-01-01

    The paper describes an image data processing system (IDAPS), its hardware/software configuration, and interactive and batch modes of operation for the analysis of the Skylab/Apollo Telescope Mount S056 X-Ray Telescope experiment data. Interactive IDAPS is primarily designed to provide on-line interactive user control of image processing operations for image familiarization, sequence and parameter optimization, and selective feature extraction and analysis. Batch IDAPS follows the normal conventions of card control and data input and output, and is best suited where the desired parameters and sequence of operations are known and when long image-processing times are required. Particular attention is given to the way in which this system has been used in solar astronomy and other investigations. Some recent results obtained by means of IDAPS are presented.

  17. Metabolomics driven analysis of artichoke leaf and its commercial products via UHPLC-q-TOF-MS and chemometrics.

    PubMed

    Farag, Mohamed A; El-Ahmady, Sherweit H; Elian, Fatma S; Wessjohann, Ludger A

    2013-11-01

    The demand to develop efficient and reliable analytical methods for the quality control of herbal medicines and nutraceuticals is on the rise, together with an increase in the legal requirements for safe and consistent levels of active principles. Here, we describe an ultra-high performance liquid chromatography method (UHPLC) coupled with quadrupole high resolution time of flight mass spectrometry (qTOF-MS) analysis for the comprehensive measurement of metabolites from three Cynara scolymus (artichoke) cultivars: American Green Globe, French Hyrious, and Egyptian Baladi. Under optimized conditions, 50 metabolites were simultaneously quantified and identified including: eight caffeic acid derivatives, six saponins, 12 flavonoids and 10 fatty acids. Principal component analysis (PCA) was used to define both similarities and differences among the three artichoke leaf cultivars. In addition, batches from seven commercially available artichoke market products were analysed and showed variable quality, particularly in caffeic acid derivatives, flavonoid and fatty acid contents. PCA analysis was able to discriminate between various preparations, including differentiation between various batches from the same supplier. To the best of our knowledge, this study provides the first approach utilizing UHPLC-MS based metabolite fingerprinting to reveal secondary metabolite compositional differences in artichoke leaf extracts. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Stimulation of electro-fermentation in single-chamber microbial electrolysis cells driven by genetically engineered anode biofilms

    NASA Astrophysics Data System (ADS)

    Awate, Bhushan; Steidl, Rebecca J.; Hamlischer, Thilo; Reguera, Gemma

    2017-07-01

    Unwanted metabolites produced during fermentations reduce titers and productivity and increase the cost of downstream purification of the targeted product. As a result, the economic feasibility of otherwise attractive fermentations is low. Using ethanol fermentation by the consolidated bioprocessing cellulolytic bacterium Cellulomonas uda, we demonstrate the effectiveness of anodic electro-fermentations at maximizing titers and productivity in a single-chamber microbial electrolysis cell (SCMEC) without the need for metabolic engineering of the fermentative microbe. The performance of the SCMEC platform relied on the genetic improvements of anode biofilms of the exoelectrogen Geobacter sulfurreducens that prevented the oxidation of cathodic hydrogen and improved lactate oxidation. Furthermore, a hybrid bioanode was designed that maximized the removal of organic acids in the fermentation broth. The targeted approach increased cellobiose consumption rates and ethanol titers, yields, and productivity three-fold or more, prevented pH imbalances and reduced batch-to-batch variability. In addition, the sugar substrate was fully consumed and ethanol was enriched in the broth during the electro-fermentation, simplifying its downstream purification. Such improvements and the possibility of scaling up SCMEC configurations highlight the potential of anodic electro-fermentations to stimulate fermentative bacteria beyond their natural capacity and to levels required for industrial implementation.

  19. Intact cell mass spectrometry as a progress tracking tool for batch and fed-batch fermentation processes.

    PubMed

    Helmel, Michaela; Marchetti-Deschmann, Martina; Raus, Martin; Posch, Andreas E; Herwig, Christoph; Šebela, Marek; Allmaier, Günter

    2015-02-01

    Penicillin production during a fermentation process using industrial strains of Penicillium chrysogenum is a research topic permanently discussed since the accidental discovery of the antibiotic. Intact cell mass spectrometry (ICMS) can be a fast and novel monitoring tool for the fermentation progress during penicillin V production in a nearly real-time fashion. This method is already used for the characterization of microorganisms and the differentiation of fungal strains; therefore, the application of ICMS to samples directly harvested from a fermenter is a promising possibility to get fast information about the progress of fungal growth. After the optimization of the ICMS method to penicillin V fermentation broth samples, the obtained ICMS data were evaluated by hierarchical cluster analysis or an in-house software solution written especially for ICMS data comparison. Growth stages of a batch and fed-batch fermentation of Penicillium chrysogenum are differentiated by one of those statistical approaches. The application of two matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) instruments in the linear positive ion mode from different vendors demonstrated the universal applicability of the developed ICMS method. The base for a fast and easy-to-use method for monitoring the fermentation progress of P. chrysogenum is created with this ICMS method developed especially for fermentation broth samples. Copyright © 2014 Elsevier Inc. All rights reserved.

  20. Combining microwave resonance technology to multivariate data analysis as a novel PAT tool to improve process understanding in fluid bed granulation.

    PubMed

    Lourenço, Vera; Herdling, Thorsten; Reich, Gabriele; Menezes, José C; Lochmann, Dirk

    2011-08-01

    A set of 192 fluid bed granulation batches at industrial scale were in-line monitored using microwave resonance technology (MRT) to determine moisture, temperature and density of the granules. Multivariate data analysis techniques such as multiway partial least squares (PLS), multiway principal component analysis (PCA) and multivariate batch control charts were applied onto collected batch data sets. The combination of all these techniques, along with off-line particle size measurements, led to significantly increased process understanding. A seasonality effect could be put into evidence that impacted further processing through its influence on the final granule size. Moreover, it was demonstrated by means of a PLS that a relation between the particle size and the MRT measurements can be quantitatively defined, highlighting a potential ability of the MRT sensor to predict information about the final granule size. This study has contributed to improve a fluid bed granulation process, and the process knowledge obtained shows that the product quality can be built in process design, following Quality by Design (QbD) and Process Analytical Technology (PAT) principles. Copyright © 2011. Published by Elsevier B.V.

  1. Recent NASA/GSFC cryogenic measurements of the total hemispheric emissivity of black surface preparations

    NASA Astrophysics Data System (ADS)

    Tuttle, J.; Canavan, E.

    2015-12-01

    High-emissivity (black) surfaces are commonly used on deep-space radiators and thermal radiation absorbers in test chambers. Since 2011 NASA Goddard Space Flight Center has measured the total hemispheric emissivity of such surfaces from 20 to 300 K using a test apparatus inside a small laboratory cryostat. We report the latest data from these measurements, including Aeroglaze Z307 paint, Black Kapton, and a configuration of painted aluminum honeycomb that was not previously tested. We also present the results of batch-to- batch reproducibility studies in Ball Infrared BlackTM and painted aluminum honeycomb. Finally, we describe a recently-adopted temperature control method which significantly speeds the data acquisition, and we discuss efforts to reduce the noise in future data.

  2. Multi-Mission Power Analysis Tool (MMPAT) Version 3

    NASA Technical Reports Server (NTRS)

    Wood, Eric G.; Chang, George W.; Chen, Fannie C.

    2012-01-01

    The Multi-Mission Power Analysis Tool (MMPAT) simulates a spacecraft power subsystem including the power source (solar array and/or radioisotope thermoelectric generator), bus-voltage control, secondary battery (lithium-ion or nickel-hydrogen), thermostatic heaters, and power-consuming equipment. It handles multiple mission types including heliocentric orbiters, planetary orbiters, and surface operations. Being parametrically driven along with its user-programmable features can reduce or even eliminate any need for software modifications when configuring it for a particular spacecraft. It provides multiple levels of fidelity, thereby fulfilling the vast majority of a project s power simulation needs throughout the lifecycle. It can operate in a stand-alone mode with a graphical user interface, in batch mode, or as a library linked with other tools. This software can simulate all major aspects of a spacecraft power subsystem. It is parametrically driven to reduce or eliminate the need for a programmer. Added flexibility is provided through user-designed state models and table-driven parameters. MMPAT is designed to be used by a variety of users, such as power subsystem engineers for sizing power subsystem components; mission planners for adjusting mission scenarios using power profiles generated by the model; system engineers for performing system- level trade studies using the results of the model during the early design phases of a spacecraft; and operations personnel for high-fidelity modeling of the essential power aspect of the planning picture.

  3. An accessible micro-capillary electrophoresis device using surface-tension-driven flow

    PubMed Central

    Mohanty, Swomitra K.; Warrick, Jay; Gorski, Jack; Beebe, David J.

    2010-01-01

    We present a rapidly fabricated micro-capillary electrophoresis chip that utilizes surface-tension-driven flow for sample injection and extraction of DNA. Surface-tension-driven flow (i.e. passive pumping) injects a fixed volume of sample that can be predicted mathematically. Passive pumping eliminates the need for tubing, valves, syringe pumps, and other equipment typically needed for interfacing with microelectrophoresis chips. This method requires a standard micropipette to load samples before separation, and remove the resulting bands after analysis. The device was made using liquid phase photopolymerization to rapidly fabricate the chip without the need of special equipment typically associated with the construction of microelectrophoresis chips (e.g. cleanroom). Batch fabrication time for the device presented here was 1.5 h including channel coating time to suppress electroosmotic flow. Devices were constructed out of poly-isobornyl acrylate and glass. A standard microscope with a UV source was used for sample detection. Separations were demonstrated using Promega BenchTop 100 bp ladder in hydroxyl ethyl cellulose (HEC) and oligonucleotides of 91 and 118 bp were used to characterize sample injection and extraction of DNA bands. The end result was an inexpensive micro-capillary electrophoresis device that uses tools (e.g. micropipette, electrophoretic power supplies, and microscopes) already present in most labs for sample manipulation and detection, making it more accessible for potential end users. PMID:19425002

  4. The influence of data-driven versus conceptually-driven processing on the development of PTSD-like symptoms.

    PubMed

    Kindt, Merel; van den Hout, Marcel; Arntz, Arnoud; Drost, Jolijn

    2008-12-01

    Ehlers and Clark [(2000). A cognitive model of posttraumatic stress disorder. Behaviour Research and Therapy, 38, 319-345] propose that a predominance of data-driven processing during the trauma predicts subsequent PTSD. We wondered whether, apart from data-driven encoding, sustained data-driven processing after the trauma is also crucial for the development of PTSD. Both hypotheses were tested in two analogue experiments. Experiment 1 demonstrated that relative to conceptually-driven processing (n=20), data-driven processing after the film (n=14), resulted in more intrusions. Experiment 2 demonstrated that relative to the neutral condition (n=24) and the data-driven encoding condition (n=24), conceptual encoding (n=25) reduced suppression of intrusions and a trend emerged for memory fragmentation. The difference between the two encoding styles was due to the beneficial effect of induced conceptual encoding and not to the detrimental effect of data-driven encoding. The data support the viability of the distinction between data-driven/conceptually-driven processing for the understanding of the development of PTSD.

  5. Impact of Nisin-Activated Packaging on Microbiota of Beef Burgers during Storage.

    PubMed

    Ferrocino, Ilario; Greppi, Anna; La Storia, Antonietta; Rantsiou, Kalliopi; Ercolini, Danilo; Cocolin, Luca

    2016-01-15

    Beef burgers were stored at 4°C in a vacuum in nisin-activated antimicrobial packaging. Microbial ecology analyses were performed on samples collected between days 0 and 21 of storage to discover the population diversity. Two batches were analyzed using RNA-based denaturing gradient gel electrophoresis (DGGE) and pyrosequencing. The active packaging retarded the growth of the total viable bacteria and lactic acid bacteria. Culture-independent analysis by pyrosequencing of RNA extracted directly from meat showed that Photobacterium phosphoreum, Lactococcus piscium, Lactobacillus sakei, and Leuconostoc carnosum were the major operational taxonomic units (OTUs) shared between control and treated samples. Beta diversity analysis of the 16S rRNA sequence data and RNA-DGGE showed a clear separation between two batches based on the microbiota. Control samples from batch B showed a significant high abundance of some taxa sensitive to nisin, such as Kocuria rhizophila, Staphylococcus xylosus, Leuconostoc carnosum, and Carnobacterium divergens, compared to control samples from batch A. However, only from batch B was it possible to find a significant difference between controls and treated samples during storage due to the active packaging. Predicted metagenomes confirmed differences between the two batches and indicated that the use of nisin-based antimicrobial packaging can determine a reduction in the abundance of specific metabolic pathways related to spoilage. The present study aimed to assess the viable bacterial communities in beef burgers stored in nisin-based antimicrobial packaging, and it highlights the efficacy of this strategy to prolong beef burger shelf life. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

  6. Study of twenty preparations of human albumin solution which failed in quality control testing due to elevated sodium content, a poor internal quality control at manufacturing unit.

    PubMed

    Prasad, J P; Madhu, Y; Singh, Surinder; Soni, G R; Agnihotri, N; Singh, Varsha; Kumar, Pradeep; Jain, Nidhi; Prakash, Anu; Singh, Varun

    2016-11-01

    Current study is conducted in our laboratory due to failure in quality control testing of twenty batches of Human Albumin solution in which sodium content is higher than the prescribed limit. These batches are received in short duration from indigenous manufacturer and is the first incident of failure of Human albumin preparation in sodium content of manufacturer. On request of manufacturer, study is conducted to rule out the cause. Repeat testing of each out of specification batch is conducted and a trend analysis is drawn between our findings and manufacturer's results, also study of trend analysis of manufacturer for the last one year. Trend analysis data indicated towards poor consistency of batches with major shift at various time intervals in sodium content of human albumin preparation. Further analysis rule out that non-traceable quality of standard used in the internal quality control testing by manufacturer is the root cause of the problem. Copyright © 2016 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.

  7. Kinetics and modeling of hexavalent chromium reduction in Enterobacter cloacae

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Yamamoto, Koji; Kato, Junichi; Yano, Takuo

    1993-01-05

    Kinetics of bacterial reduction of toxic hexavalent chromium (chromate: CrO[sub 4][sup [minus]2]) was investigated using batch and fed-batch cultures of Enterobacter cloacae strain HO1. In fed-batch cultures, the CrO[sub 4][sup [minus]2] feed was controlled on the basis of the rate of pH change. This control strategy has proven to be useful for avoiding toxic CrO[sub 3][sup [minus]2] overload. A simple mathematical model was developed to describe the bacterial process of CrO[sub 4][sup [minus]2] reduction. In this model, two types of bacterial cells were considered: induced, CrO[sub 4][sup [minus]2]-resistant cells and uninduced, sensitive ones. Only resistant cells were assumed to bemore » able to reduce CrO[sub 4][sup [minus]2]. These fundamental ideas were supported by the model predictions which well approximated all experimental data. In a simulation study, the model was also used to optimize fed-batch cultures, instead of lengthy and expensive laboratory experiments.« less

  8. A novel process-based model of microbial growth: self-inhibition in Saccharomyces cerevisiae aerobic fed-batch cultures.

    PubMed

    Mazzoleni, Stefano; Landi, Carmine; Cartenì, Fabrizio; de Alteriis, Elisabetta; Giannino, Francesco; Paciello, Lucia; Parascandola, Palma

    2015-07-30

    Microbial population dynamics in bioreactors depend on both nutrients availability and changes in the growth environment. Research is still ongoing on the optimization of bioreactor yields focusing on the increase of the maximum achievable cell density. A new process-based model is proposed to describe the aerobic growth of Saccharomyces cerevisiae cultured on glucose as carbon and energy source. The model considers the main metabolic routes of glucose assimilation (fermentation to ethanol and respiration) and the occurrence of inhibition due to the accumulation of both ethanol and other self-produced toxic compounds in the medium. Model simulations reproduced data from classic and new experiments of yeast growth in batch and fed-batch cultures. Model and experimental results showed that the growth decline observed in prolonged fed-batch cultures had to be ascribed to self-produced inhibitory compounds other than ethanol. The presented results clarify the dynamics of microbial growth under different feeding conditions and highlight the relevance of the negative feedback by self-produced inhibitory compounds on the maximum cell densities achieved in a bioreactor.

  9. QQACCT

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Jacobsen, Douglas

    2015-01-01

    batchacct provides convenient library and command-line access to batch system accounting data for GridEngine and SLURM schedulers. It can be used to perform queries useful for data analysis of the accounting data alone or for integrative analysis in the context of a larger query.

  10. Recommendations of the VAC2VAC workshop on the design of multi-centre validation studies.

    PubMed

    Halder, Marlies; Depraetere, Hilde; Delannois, Frédérique; Akkermans, Arnoud; Behr-Gross, Marie-Emmanuelle; Bruysters, Martijn; Dierick, Jean-François; Jungbäck, Carmen; Kross, Imke; Metz, Bernard; Pennings, Jeroen; Rigsby, Peter; Riou, Patrice; Balks, Elisabeth; Dobly, Alexandre; Leroy, Odile; Stirling, Catrina

    2018-03-01

    Within the Innovative Medicines Initiative 2 (IMI 2) project VAC2VAC (Vaccine batch to vaccine batch comparison by consistency testing), a workshop has been organised to discuss ways of improving the design of multi-centre validation studies and use the data generated for product-specific validation purposes. Moreover, aspects of validation within the consistency approach context were addressed. This report summarises the discussions and outlines the conclusions and recommendations agreed on by the workshop participants. Copyright © 2018.

  11. Integrated approaches to the application of advanced modeling technology in process development and optimization

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Allgor, R.J.; Feehery, W.F.; Tolsma, J.E.

    The batch process development problem serves as good candidate to guide the development of process modeling environments. It demonstrates that very robust numerical techniques are required within an environment that can collect, organize, and maintain the data and models required to address the batch process development problem. This paper focuses on improving the robustness and efficiency of the numerical algorithms required in such a modeling environment through the development of hybrid numerical and symbolic strategies.

  12. Study on the Association between Tail Lesion Score, Cold Carcass Weight, and Viscera Condemnations in Slaughter Pigs

    PubMed Central

    Teixeira, Dayane Lemos; Harley, Sarah; Hanlon, Alison; O’Connell, Niamh Elizabeth; More, Simon John; Manzanilla, Edgar Garcia; Boyle, Laura Ann

    2016-01-01

    The aim of this study was to assess the relationship between tail lesions, cold carcass weight, and viscera condemnations in an Irish abattoir. The following data were collected at the evisceration point from every third pig slaughtered over 7 days: farm identification, sex, tail lesion score, viscera inspection outcome, and cold carcass weight. Tail lesions were scored according to a 5-point scale. Disease lesions responsible for lung (pleurisy, pneumonia, and abscess), heart (pericarditis), and liver (ascariasis) condemnation were recorded based on the decision of the veterinary inspector (VI). Data on 3,143 pigs from 61 batches were available. The relationship between disease lesions, tail lesion score, and cold carcass weight was studied at individual carcass level, while the relationship between disease lesions and tail lesion score was studied at both carcass and batch level. Tail lesions (score ≥1) were found in 72% of the study population, with 2.3% affected by severe tail lesions (scores ≥3). Pleurisy (13.7%) followed by pneumonia (10.4%) showed the highest prevalence, whereas the prevalence of ascariasis showed the greatest variation between batches (0–75%). Tail lesion score, pleurisy, pleuropneumonia, and pericarditis were associated with reductions in carcass cold weight (P ≤ 0.05) ranging from 3 to 6.6 kg. Tail lesion score was associated with condemnations for pleurisy, pneumonia, and pleuropneumonia (P ≤ 0.05) at a batch level. VI shift was associated with condemnations for pneumonia, pleuropneumonia, and pericarditis (P ≤ 0.05) at a carcass level and with pneumonia at a batch level. Sex was not associated with viscera condemnations but males were more likely to be affected by tail lesions. The relationship between overall tail lesion score and the lung diseases at batch level supports the relationship between poor health and poor welfare of pigs on farms. The inclusion of tail lesion scores at post-mortem meat inspection should be considered as a health and welfare diagnostic tool. PMID:27014706

  13. Hadoop distributed batch processing for Gaia: a success story

    NASA Astrophysics Data System (ADS)

    Riello, Marco

    2015-12-01

    The DPAC Cambridge Data Processing Centre (DPCI) is responsible for the photometric calibration of the Gaia data including the low resolution spectra. The large data volume produced by Gaia (~26 billion transits/year), the complexity of its data stream and the self-calibrating approach pose unique challenges for scalability, reliability and robustness of both the software pipelines and the operations infrastructure. DPCI has been the first in DPAC to realise the potential of Hadoop and Map/Reduce and to adopt them as the core technologies for its infrastructure. This has proven a winning choice allowing DPCI unmatched processing throughput and reliability within DPAC to the point that other DPCs have started following our footsteps. In this talk we will present the software infrastructure developed to build the distributed and scalable batch data processing system that is currently used in production at DPCI and the excellent results in terms of performance of the system.

  14. Medication Waste Reduction in Pediatric Pharmacy Batch Processes

    PubMed Central

    Veltri, Michael A.; Hamrock, Eric; Mollenkopf, Nicole L.; Holt, Kristen; Levin, Scott

    2014-01-01

    OBJECTIVES: To inform pediatric cart-fill batch scheduling for reductions in pharmaceutical waste using a case study and simulation analysis. METHODS: A pre and post intervention and simulation analysis was conducted during 3 months at a 205-bed children's center. An algorithm was developed to detect wasted medication based on time-stamped computerized provider order entry information. The algorithm was used to quantify pharmaceutical waste and associated costs for both preintervention (1 batch per day) and postintervention (3 batches per day) schedules. Further, simulation was used to systematically test 108 batch schedules outlining general characteristics that have an impact on the likelihood for waste. RESULTS: Switching from a 1-batch-per-day to a 3-batch-per-day schedule resulted in a 31.3% decrease in pharmaceutical waste (28.7% to 19.7%) and annual cost savings of $183,380. Simulation results demonstrate how increasing batch frequency facilitates a more just-in-time process that reduces waste. The most substantial gains are realized by shifting from a schedule of 1 batch per day to at least 2 batches per day. The simulation exhibits how waste reduction is also achievable by avoiding batch preparation during daily time periods where medication administration or medication discontinuations are frequent. Last, the simulation was used to show how reducing batch preparation time per batch provides some, albeit minimal, opportunity to decrease waste. CONCLUSIONS: The case study and simulation analysis demonstrate characteristics of batch scheduling that may support pediatric pharmacy managers in redesign toward minimizing pharmaceutical waste. PMID:25024671

  15. Medication waste reduction in pediatric pharmacy batch processes.

    PubMed

    Toerper, Matthew F; Veltri, Michael A; Hamrock, Eric; Mollenkopf, Nicole L; Holt, Kristen; Levin, Scott

    2014-04-01

    To inform pediatric cart-fill batch scheduling for reductions in pharmaceutical waste using a case study and simulation analysis. A pre and post intervention and simulation analysis was conducted during 3 months at a 205-bed children's center. An algorithm was developed to detect wasted medication based on time-stamped computerized provider order entry information. The algorithm was used to quantify pharmaceutical waste and associated costs for both preintervention (1 batch per day) and postintervention (3 batches per day) schedules. Further, simulation was used to systematically test 108 batch schedules outlining general characteristics that have an impact on the likelihood for waste. Switching from a 1-batch-per-day to a 3-batch-per-day schedule resulted in a 31.3% decrease in pharmaceutical waste (28.7% to 19.7%) and annual cost savings of $183,380. Simulation results demonstrate how increasing batch frequency facilitates a more just-in-time process that reduces waste. The most substantial gains are realized by shifting from a schedule of 1 batch per day to at least 2 batches per day. The simulation exhibits how waste reduction is also achievable by avoiding batch preparation during daily time periods where medication administration or medication discontinuations are frequent. Last, the simulation was used to show how reducing batch preparation time per batch provides some, albeit minimal, opportunity to decrease waste. The case study and simulation analysis demonstrate characteristics of batch scheduling that may support pediatric pharmacy managers in redesign toward minimizing pharmaceutical waste.

  16. Experience on HTCondor batch system for HEP and other research fields at KISTI-GSDC

    NASA Astrophysics Data System (ADS)

    Ahn, S. U.; Jaikar, A.; Kong, B.; Yeo, I.; Bae, S.; Kim, J.

    2017-10-01

    Global Science experimental Data hub Center (GSDC) at Korea Institute of Science and Technology Information (KISTI) located at Daejeon in South Korea is the unique datacenter in the country which helps with its computing resources fundamental research fields dealing with the large-scale of data. For historical reason, it has run Torque batch system while recently it starts running HTCondor for new systems. Having different kinds of batch systems implies inefficiency in terms of resource management and utilization. We conducted a research on resource management with HTCondor for several user scenarios corresponding to the user environments that currently GSDC supports. A recent research on the resource usage patterns at GSDC is considered in this research to build the possible user scenarios. Checkpointing and Super-Collector model of HTCondor give us more efficient and flexible way to manage resources and Grid Gate provided by HTCondor helps to interface with the Grid environment. In this paper, the overview on the essential features of HTCondor exploited in this work is described and the practical examples for HTCondor cluster configuration in our cases are presented.

  17. Batch and fixed bed adsorption of levofloxacin on granular activated carbon from date (Phoenix dactylifera L.) stones by KOH chemical activation.

    PubMed

    Darweesh, Teeba M; Ahmed, Muthanna J

    2017-03-01

    Granular activated carbon (KAC) was prepared from abundant Phoenix dactylifera L. stones by microwave- assisted KOH activation. The characteristics of KAC were tested by pore analyses, scanning electron microscopy (SEM) and Fourier transforms infrared spectroscopy (FTIR). The adsorption behavior of levofloxacin (LEV) antibiotic on KAC with surface area of 817m 2 /g and pore volume of 0.638cm 3 /g were analyzed using batch and fixed bed systems. The equilibrium data collected by batch experiments were well fitted with Langmuir compared to Freundlich and Temkin isotherms. The effect of flow rate (0.5-1.5ml/min), bed height (15-25cm), and initial LEV concentration (75-225mg/l) on the behavior of breakthrough curves was explained. The fixed bed analysis showed the better correlation of breakthrough data by both Thomas and Yoon-Nelson models. High LEV adsorption capacity of 100.3mg/g was reported on KAC, thus being an efficient adsorbent for antibiotic pollutants to protect ecological systems. Copyright © 2017 Elsevier B.V. All rights reserved.

  18. Use of Model-Based Nutrient Feeding for Improved Production of Artemisinin by Hairy Roots of Artemisia Annua in a Modified Stirred Tank Bioreactor.

    PubMed

    Patra, Nivedita; Srivastava, Ashok K

    2015-09-01

    Artemisinin has been indicated to be a potent drug for the cure of malaria. Batch growth and artemisinin production kinetics of hairy root cultures of Artemisia annua were studied under shake flask conditions which resulted in accumulation of 12.49 g/L biomass and 0.27 mg/g artemisinin. Using the kinetic data, a mathematical model was identified to understand and optimize the system behavior. The developed model was then extrapolated to design nutrient feeding strategies during fed-batch cultivation for enhanced production of artemisinin. In one of the fed-batch cultivation, sucrose (37 g/L) feeding was done at a constant feed rate of 0.1 L/day during 10-15 days, which led to improved artemisinin accumulation of 0.77 mg/g. The second strategy of fed-batch hairy root cultivation involved maintenance of pseudo-steady state sucrose concentration (20.8 g/L) during 10-15 days which resulted in artemisinin accumulation of 0.99 mg/g. Fed-batch cultivation (with the maintenance of pseudo-steady state of substrate) of Artemisia annua hairy roots was, thereafter, implemented in bioreactor cultivation, which featured artemisinin accumulation of 1.0 mg/g artemisinin in 16 days of cultivation. This is the highest reported artemisinin yield by hairy root cultivation in a bioreactor.

  19. Enhancing tablet disintegration characteristics of a highly water-soluble high-drug-loading formulation by granulation process.

    PubMed

    Pandey, Preetanshu; Levins, Christopher; Pafiakis, Steve; Zacour, Brian; Bindra, Dilbir S; Trinh, Jade; Buckley, David; Gour, Shruti; Sharif, Shasad; Stamato, Howard

    2018-07-01

    The objective of this study was to improve the disintegration and dissolution characteristics of a highly water-soluble tablet matrix by altering the manufacturing process. A high disintegration time along with high dependence of the disintegration time on tablet hardness was observed for a high drug loading (70% w/w) API when formulated using a high-shear wet granulation (HSWG) process. Keeping the formulation composition mostly constant, a fluid-bed granulation (FBG) process was explored as an alternate granulation method using a 2 (4-1) fractional factorial design with two center points. FBG batches (10 batches) were manufactured using varying disingtegrant amount, spray rate, inlet temperature (T) and atomization air pressure. The resultant final blend particle size was affected significantly by spray rate (p = .0009), inlet T (p = .0062), atomization air pressure (p = .0134) and the interaction effect between inlet T*spray rate (p = .0241). The compactibility of the final blend was affected significantly by disintegrant amount (p < .0001), atomization air pressure (p = .0013) and spray rate (p = .05). It was observed that the fluid-bed batches gave significantly lower disintegration times than the HSWG batches, and mercury intrusion porosimetry data revealed that this was caused by the higher internal pore structure of tablets manufactured using the FBG batches.

  20. Model-Based Nutrient Feeding Strategies for the Increased Production of Polyhydroxybutyrate (PHB) by Alcaligenes latus.

    PubMed

    Gahlawat, Geeta; Srivastava, Ashok K

    2017-10-01

    Polyhydroxyalkanoates (PHAs) are biodegradable polymers which are considered as an effective alternative for conventional plastics due to their mechanical properties similar to the latter. However, the widespread use of these polymers is still hampered due to their higher cost of production as compared to plastics. The production cost could be overcome by obtaining high yields and productivity. The goal of the present research was to enhance the yield of polyhydroxybutyrate (PHB) with the help of two simple fed-batch cultivation strategies. In the present study, average batch kinetic and substrate limitation/inhibition study data of Alcaligenes latus was used for the development of PHB model which was then adopted for designing various off-line nutrient feeding strategies to enhance PHB accumulation. The predictive ability of the model was validated by experimental implementation of two fed-batch strategies. One such dynamic strategy of fed-batch cultivation under pseudo-steady state with respect to nitrogen and simultaneous carbon feeding strategy resulted in significantly high biomass and PHB concentration of 39.17 g/L and 29.64 g/L, respectively. This feeding strategy demonstrated a high PHB productivity and PHB content of 0.6 g/L h and 75%, respectively, which were remarkably high in comparison to batch cultivation. The mathematical model can also be employed for designing various other nutrient feeding strategies.

  1. Acceptance Test Data for BWXT Coated Particle Batch 93164A Defective IPyC Fraction and Pyrocarbon Anisotropy

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Helmreich, Grant W.; Hunn, John D.; Skitt, Darren J.

    2017-02-01

    Coated particle fuel batch J52O-16-93164 was produced by Babcock and Wilcox Technologies (BWXT) for possible selection as fuel for the Advanced Gas Reactor Fuel Development and Qualification (AGR) Program’s AGR-5/6/7 irradiation test in the Idaho National Laboratory (INL) Advanced Test Reactor (ATR), or may be used as demonstration production-scale coated particle fuel for other experiments. The tristructural-isotropic (TRISO) coatings were deposited in a 150-mm-diameter production-scale fluidizedbed chemical vapor deposition (CVD) furnace onto 425-μm-nominal-diameter spherical kernels from BWXT lot J52L-16-69316. Each kernel contained a mixture of 15.5%-enriched uranium carbide and uranium oxide (UCO) and was coated with four consecutive CVD layers:more » a ~50% dense carbon buffer layer with 100-μm-nominal thickness, a dense inner pyrolytic carbon (IPyC) layer with 40-μm-nominal thickness, a silicon carbide (SiC) layer with 35-μm-nominal thickness, and a dense outer pyrolytic carbon (OPyC) layer with 40-μm-nominal thickness. The TRISO-coated particle batch was sieved to upgrade the particles by removing over-sized and under-sized material, and the upgraded batch was designated by appending the letter A to the end of the batch number (i.e., 93164A).« less

  2. A direct comparison of U.S. Environmental Protection Agency's method 304B and batch tests for determining activated-sludge biodegradation rate constants for volatile organic compounds

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Cano, M.L.; Wilcox, M.E.; Compernolle, R. van

    Biodegradation rate constants for volatile organic compounds (VOCs) in activated-sludge systems are needed to quantify emissions. One current US environmental Protection Agency method for determining a biodegradation rate constant is Method 304B. In this approach, a specific activated-sludge unit is simulated by a continuous biological treatment system with a sealed headspace. Batch experiments, however, can be alternatives to Method 304B. Two of these batch methods are the batch test that uses oxygen addition (BOX) and the serum bottle test (SBT). In this study, Method 304B was directly compared to BOX and SBT experiments. A pilot-scale laboratory reactor was constructed tomore » serve as the Method 304B unit. Biomass from the unit was also used to conduct BOX and modified SBT experiments (modification involved use of a sealed draft-tube reactor with a headspace recirculation pump instead of a serum bottle) for 1,2-dichloroethane, diisopropyl ether, methyl tertiary butyl ether, and toluene. Three experimental runs--each consisting of one Method 304B experiment, one BOX experiment, and one modified SBT experiment--were completed. The BOX and SBT data for each run were analyzed using a Monod model, and best-fit biodegradation kinetic parameters were determined for each experiment, including a first-order biodegradation rate constant (K{sub 1}). Experimental results suggest that for readily biodegradable VOCs the two batch techniques can provide improved means of determining biodegradation rate constants compared with Method 304B. In particular, these batch techniques avoid the Method 304B problem associated with steady-state effluent concentrations below analytical detection limits. However, experimental results also suggest that the two batch techniques should not be used to determine biodegradation rate constants for slowly degraded VOCs (i.e., K{sub 1} {lt} 0.1 L/g VSS-h).« less

  3. Chemical and Toxicological Fate of Fumonisin B1 during Extrusion Processing of Corn Grits

    USDA-ARS?s Scientific Manuscript database

    Two batches of flaking corn grits were prepared by growing Fusarium verticillioides to contain low and high levels of fumonisin B1 (FB1), Batch-1 at 9.7 ppm and Batch-2 at 50 ppm FB1 as determined by HPLC. These two batches were extruded (Batch-1E; Batch-2E) or extruded with 10% w/w glucose supplem...

  4. Development of In Vitro-In Vivo Correlation/Relationship Modeling Approaches for Immediate Release Formulations Using Compartmental Dynamic Dissolution Data from “Golem”: A Novel Apparatus

    PubMed Central

    Tuszyński, Paweł K.; Polak, Sebastian; Jachowicz, Renata; Mendyk, Aleksander; Dohnal, Jiří

    2015-01-01

    Different batches of atorvastatin, represented by two immediate release formulation designs, were studied using a novel dynamic dissolution apparatus, simulating stomach and small intestine. A universal dissolution method was employed which simulated the physiology of human gastrointestinal tract, including the precise chyme transit behavior and biorelevant conditions. The multicompartmental dissolution data allowed direct observation and qualitative discrimination of the differences resulting from highly pH dependent dissolution behavior of the tested batches. Further evaluation of results was performed using IVIVC/IVIVR development. While satisfactory correlation could not be achieved using a conventional deconvolution based-model, promising results were obtained through the use of a nonconventional approach exploiting the complex compartmental dissolution data. PMID:26120580

  5. Annual ADP planning document

    NASA Technical Reports Server (NTRS)

    Mogilevsky, M.

    1973-01-01

    The Category A computer systems at KSC (Al and A2) which perform scientific and business/administrative operations are described. This data division is responsible for scientific requirements supporting Saturn, Atlas/Centaur, Titan/Centaur, Titan III, and Delta vehicles, and includes realtime functions, Apollo-Soyuz Test Project (ASTP), and the Space Shuttle. The work is performed chiefly on the GEL-635 (Al) system located in the Central Instrumentation Facility (CIF). The Al system can perform computations and process data in three modes: (1) real-time critical mode; (2) real-time batch mode; and (3) batch mode. The Division's IBM-360/50 (A2) system, also at the CIF, performs business/administrative data processing such as personnel, procurement, reliability, financial management and payroll, real-time inventory management, GSE accounting, preventive maintenance, and integrated launch vehicle modification status.

  6. B827 Chemical Synthhesis Project - Industrial Control System Integration - Statement of Work & Specification with Attachments 1-14

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Wade, F. E.

    The Chemical Synthesis Pilot Process at the Lawrence Livermore National Laboratory (LLNL) Site 300 827 Complex will be used to synthesize small quantities of material to support research and development. The project will modernize and increase current capabilities for chemical synthesis at LLNL. The primary objective of this project is the conversion of a non-automated hands-on process to a remoteoperation process, while providing enhanced batch process step control, stored recipe-specific parameter sets, process variable visibility, monitoring, alarm and warning handling, and comprehensive batch record data logging. This Statement of Work and Specification provides the industrial-grade process control requirements for themore » chemical synthesis batching control system, hereafter referred to as the “Control System” to be delivered by the System Integrator.« less

  7. Evaluation of TDRSS-user orbit determination accuracy using batch least-squares and sequential methods

    NASA Technical Reports Server (NTRS)

    Oza, D. H.; Jones, T. L.; Hodjatzadeh, M.; Samii, M. V.; Doll, C. E.; Hart, R. C.; Mistretta, G. D.

    1991-01-01

    The development of the Real-Time Orbit Determination/Enhanced (RTOD/E) system as a prototype system for sequential orbit determination on a Disk Operating System (DOS) based Personal Computer (PC) is addressed. The results of a study to compare the orbit determination accuracy of a Tracking and Data Relay Satellite System (TDRSS) user spacecraft obtained using RTOD/E with the accuracy of an established batch least squares system, the Goddard Trajectory Determination System (GTDS), is addressed. Independent assessments were made to examine the consistencies of results obtained by the batch and sequential methods. Comparisons were made between the forward filtered RTOD/E orbit solutions and definitive GTDS orbit solutions for the Earth Radiation Budget Satellite (ERBS); the maximum solution differences were less than 25 m after the filter had reached steady state.

  8. MethLAB

    PubMed Central

    Kilaru, Varun; Barfield, Richard T; Schroeder, James W; Smith, Alicia K

    2012-01-01

    Recent evidence suggests that DNA methylation changes may underlie numerous complex traits and diseases. The advent of commercial, array-based methods to interrogate DNA methylation has led to a profusion of epigenetic studies in the literature. Array-based methods, such as the popular Illumina GoldenGate and Infinium platforms, estimate the proportion of DNA methylated at single-base resolution for thousands of CpG sites across the genome. These arrays generate enormous amounts of data, but few software resources exist for efficient and flexible analysis of these data. We developed a software package called MethLAB (http://genetics.emory.edu/conneely/MethLAB) using R, an open source statistical language that can be edited to suit the needs of the user. MethLAB features a graphical user interface (GUI) with a menu-driven format designed to efficiently read in and manipulate array-based methylation data in a user-friendly manner. MethLAB tests for association between methylation and relevant phenotypes by fitting a separate linear model for each CpG site. These models can incorporate both continuous and categorical phenotypes and covariates, as well as fixed or random batch or chip effects. MethLAB accounts for multiple testing by controlling the false discovery rate (FDR) at a user-specified level. Standard output includes a spreadsheet-ready text file and an array of publication-quality figures. Considering the growing interest in and availability of DNA methylation data, there is a great need for user-friendly open source analytical tools. With MethLAB, we present a timely resource that will allow users with no programming experience to implement flexible and powerful analyses of DNA methylation data. PMID:22430798

  9. Evaluation of Landsat-4 orbit determination accuracy using batch least-squares and sequential methods

    NASA Astrophysics Data System (ADS)

    Oza, D. H.; Jones, T. L.; Feiertag, R.; Samii, M. V.; Doll, C. E.; Mistretta, G. D.; Hart, R. C.

    The Goddard Space Flight Center (GSFC) Flight Dynamics Division (FDD) commissioned Applied Technology Associates, Incorporated, to develop the Real-Time Orbit Determination/Enhanced (RTOD/E) system on a Disk Operating System (DOS)-based personal computer (PC) as a prototype system for sequential orbit determination of spacecraft. This paper presents the results of a study to compare the orbit determination accuracy for a Tracking and Data Relay Satellite (TDRS) System (TDRSS) user spacecraft, Landsat-4, obtained using RTOD/E, operating on a PC, with the accuracy of an established batch least-squares system, the Goddard Trajectory Determination System (GTDS), operating on a mainframe computer. The results of Landsat-4 orbit determination will provide useful experience for the Earth Observing System (EOS) series of satellites. The Landsat-4 ephemerides were estimated for the May 18-24, 1992, timeframe, during which intensive TDRSS tracking data for Landsat-4 were available. During this period, there were two separate orbit-adjust maneuvers on one of the TDRSS spacecraft (TDRS-East) and one small orbit-adjust maneuver for Landsat-4. Independent assessments were made of the consistencies (overlap comparisons for the batch case and covariances and the first measurement residuals for the sequential case) of solutions produced by the batch and sequential methods. The forward-filtered RTOD/E orbit solutions were compared with the definitive GTDS orbit solutions for Landsat-4; the solution differences were generally less than 30 meters after the filter had reached steady state.

  10. GO, an exec for running the programs: CELL, COLLIDER, MAGIC, PATRICIA, PETROS, TRANSPORT, and TURTLE

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shoaee, H.

    1982-05-01

    An exec has been written and placed on the PEP group's public disk to facilitate the use of several PEP related computer programs available on VM. The exec's program list currently includes: CELL, COLLIDER, MAGIC, PATRICIA, PETROS, TRANSPORT, and TURTLE. In addition, provisions have been made to allow addition of new programs to this list as they become available. The GO exec is directly callable from inside the Wylbur editor (in fact, currently this is the only way to use the GO exec.). It provides the option of running any of the above programs in either interactive or batch mode.more » In the batch mode, the GO exec sends the data in the Wylbur active file along with the information required to run the job to the batch monitor (BMON, a virtual machine that schedules and controls execution of batch jobs). This enables the user to proceed with other VM activities at his/her terminal while the job executes, thus making it of particular interest to the users with jobs requiring much CPU time to execute and/or those wishing to run multiple jobs independently. In the interactive mode, useful for small jobs requiring less CPU time, the job is executed by the user's own Virtual Machine using the data in the active file as input. At the termination of an interactive job, the GO exec facilitates examination of the output by placing it in the Wylbur active file.« less

  11. GO, an exec for running the programs: CELL, COLLIDER, MAGIC, PATRICIA, PETROS, TRANSPORT and TURTLE

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Shoaee, H.

    1982-05-01

    An exec has been written and placed on the PEP group's public disk (PUBRL 192) to facilitate the use of several PEP related computer programs available on VM. The exec's program list currently includes: CELL, COLLIDER, MAGIC, PATRICIA, PETROS, TRANSPORT, and TURTLE. In addition, provisions have been made to allow addition of new programs to this list as they become available. The GO exec is directly callable from inside the Wylbur editor (in fact, currently this is the only way to use the GO exec.) It provides the option of running any of the above programs in either interactive ormore » batch mode. In the batch mode, the GO exec sends the data in the Wylbur active file along with the information required to run the job to the batch monitor (BMON, a virtual machine that schedules and controls execution of batch jobs). This enables the user to proceed with other VM activities at his/her terminal while the job executes, thus making it of particular interest to the users with jobs requiring much CPU time to execute and/or those wishing to run multiple jobs independently. In the interactive mode, useful for small jobs requiring less CPU time, the job is executed by the user's own Virtual Machine using the data in the active file as input. At the termination of an interactive job, the GO exec facilitates examination of the output by placing it in the Wylbur active file.« less

  12. Actual Waste Demonstration of the Nitric-Glycolic Flowsheet for Sludge Batch 9 Qualification

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    J. D. Newell; Pareizs, J. M.; Martino, C. J.

    For each sludge batch that is processed in the Defense Waste Processing Facility (DWPF), the Savannah River National Laboratory (SRNL) performs qualification testing to demonstrate that the sludge batch is processable. Testing performed by the Savannah River National Laboratory has shown glycolic acid to be effective in replacing the function of formic acid in the DWPF chemical process. The nitric-glycolic flowsheet reduces mercury, significantly lowers the catalytic generation of hydrogen and ammonia which could allow purge reduction in the Sludge Receipt and Adjustment Tank (SRAT), stabilizes the pH and chemistry in the SRAT and the Slurry Mix Evaporator (SME), allowsmore » for effective rheology adjustment, and is favorable with respect to melter flammability. In order to implement the new flowsheet, SRAT and SME cycles, designated SC-18, were performed using a Sludge Batch (SB) 9 slurry blended from SB8 Tank 40H and Tank 51H samples. The SRAT cycle involved adding nitric and glycolic acids to the sludge, refluxing to steam strip mercury, and dewatering to a targeted solids concentration. Data collected during the SRAT cycle included offgas analyses, process temperatures, heat transfer, and pH measurements. The SME cycle demonstrated the addition of glass frit and the replication of six canister decontamination additions. The demonstration concluded with dewatering to a targeted solids concentration. Data collected during the SME cycle included offgas analyses, process temperatures, heat transfer, and pH measurements. Slurry and condensate samples were collected for subsequent analysis« less

  13. Evaluation of Landsat-4 orbit determination accuracy using batch least-squares and sequential methods

    NASA Technical Reports Server (NTRS)

    Oza, D. H.; Jones, T. L.; Feiertag, R.; Samii, M. V.; Doll, C. E.; Mistretta, G. D.; Hart, R. C.

    1993-01-01

    The Goddard Space Flight Center (GSFC) Flight Dynamics Division (FDD) commissioned Applied Technology Associates, Incorporated, to develop the Real-Time Orbit Determination/Enhanced (RTOD/E) system on a Disk Operating System (DOS)-based personal computer (PC) as a prototype system for sequential orbit determination of spacecraft. This paper presents the results of a study to compare the orbit determination accuracy for a Tracking and Data Relay Satellite (TDRS) System (TDRSS) user spacecraft, Landsat-4, obtained using RTOD/E, operating on a PC, with the accuracy of an established batch least-squares system, the Goddard Trajectory Determination System (GTDS), operating on a mainframe computer. The results of Landsat-4 orbit determination will provide useful experience for the Earth Observing System (EOS) series of satellites. The Landsat-4 ephemerides were estimated for the May 18-24, 1992, timeframe, during which intensive TDRSS tracking data for Landsat-4 were available. During this period, there were two separate orbit-adjust maneuvers on one of the TDRSS spacecraft (TDRS-East) and one small orbit-adjust maneuver for Landsat-4. Independent assessments were made of the consistencies (overlap comparisons for the batch case and covariances and the first measurement residuals for the sequential case) of solutions produced by the batch and sequential methods. The forward-filtered RTOD/E orbit solutions were compared with the definitive GTDS orbit solutions for Landsat-4; the solution differences were generally less than 30 meters after the filter had reached steady state.

  14. The influence of habitat structure on energy allocation tactics in an estuarine batch spawner

    NASA Astrophysics Data System (ADS)

    Brigolin, D.; Cavraro, F.; Zanatta, V.; Pastres, R.; Malavasi, S.

    2016-04-01

    Trade-off between fecundity and survival was tested in a batch spawner, the Mediterranean killifish Aphanius fasciatus, using an integrated modelling-data approach based on previously collected empirical data. Two sites of the lagoon of Venice (Northern Adriatic sea, Italy) were selected in order to compare the energy allocation between growth and reproduction in two contrasting habitats. These were characterised by high and comparable level of richness in basal resources, but showed two different mortality schedules: an open natural salt marsh, exposed to high level of predation, and a confined artificial site protected from piscivorous predation. By means of a bioenergetic Scope for Growth model, developed and calibrated for the specific goals of this work, we compared the average individual life history between the two habitats. The average individual life history is characterised by a higher number of spawning events and lower per-spawning investment in the confined site exposed to lower predation risk, compared to the site connected with the open lagoon. Thus, model predictions suggest that habitat structure with different extrinsic mortality schedules may shape the life history strategy in modulating the pattern of energy allocation. Model application highlights the central role of energy partitioning through batch spawning, in determining the life history strategy. The particular ovary structure of a batch spawner seems therefore to allow the fish to modulate timing and investment of spawning events, shaping the optimal life history in relation to the environmental conditions.

  15. Portability studies of modular data base managers. Interim reports. [Running CDC's DATATRAN 2 on IBM 360/370 and IBM's JOSHUA on CDC computers

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Kopp, H.J.; Mortensen, G.A.

    1978-04-01

    Approximately 60% of the full CDC 6600/7600 Datatran 2.0 capability was made operational on IBM 360/370 equipment. Sufficient capability was made operational to demonstrate adequate performance for modular program linking applications. Also demonstrated were the basic capabilities and performance required to support moderate-sized data base applications and moderately active scratch input/output applications. Approximately one to two calendar years are required to develop DATATRAN 2.0 capabilities fully for the entire spectrum of applications proposed. Included in the next stage of conversion should be syntax checking and syntax conversion features that would foster greater FORTRAN compatibility between IBM and CDC developed modules.more » The batch portion of the JOSHUA Modular System, which was developed by Savannah River Laboratory to run on an IBM computer, was examined for the feasibility of conversion to run on a Control Data Corporation (CDC) computer. Portions of the JOSHUA Precompiler were changed so as to be operable on the CDC computer. The Data Manager and Batch Monitor were also examined for conversion feasibility, but no changes were made in them. It appears to be feasible to convert the batch portion of the JOSHUA Modular System to run on a CDC computer with an estimated additional two to three man-years of effort. 9 tables.« less

  16. Comparative meta-analysis and experimental kinetic investigation of column and batch bottle microcosm treatability studies informing in situ groundwater remedial design.

    PubMed

    Driver, Erin M; Roberts, Jeff; Dollar, Peter; Charles, Maurissa; Hurst, Paul; Halden, Rolf U

    2017-02-05

    A systematic comparison was performed between batch bottle and continuous-flow column microcosms (BMs and CMs, respectively) commonly used for in situ groundwater remedial design. Review of recent literature (2000-2014) showed a preference for reporting batch kinetics, even when corresponding column data were available. Additionally, CMs produced higher observed rate constants, exceeding those of BMs by a factor of 6.1±1.1 standard error. In a subsequent laboratory investigation, 12 equivalent microcosm pairs were constructed from fractured bedrock and perchloroethylene (PCE) impacted groundwater. First-order PCE transformation kinetics of CMs were 8.0±4.8 times faster than BMs (rates: 1.23±0.87 vs. 0.16±0.05d -1 , respectively). Additionally, CMs transformed 16.1±8.0-times more mass than BMs owing to continuous-feed operation. CMs are concluded to yield more reliable kinetic estimates because of much higher data density stemming from long-term, steady-state conditions. Since information from BMs and CMs is valuable and complementary, treatability studies should report kinetic data from both when available. This first systematic investigation of BMs and CMs highlights the need for a more unified framework for data use and reporting in treatability studies informing decision-making for field-scale groundwater remediation. Copyright © 2016 Elsevier B.V. All rights reserved.

  17. Competitive adsorption and selectivity sequence of heavy metals by chicken bone-derived biochar: Batch and column experiment.

    PubMed

    Park, Jong-Hwan; Cho, Ju-Sik; Ok, Yong Sik; Kim, Seong-Heon; Kang, Se-Won; Choi, Ik-Won; Heo, Jong-Soo; DeLaune, Ronald D; Seo, Dong-Cheol

    2015-01-01

    The objective of this research was to evaluate adsorption of heavy metals in single- and ternary-metal forms onto chicken bone biochar (CBB). Competitive sorption of heavy metals by CBB has never been reported previously. The maximum adsorption capacities of metals by CBB were in the order of Cu (130 mg g(-1)) > Cd (109 mg g(-1)) > Zn (93 mg g(-1)) in the single-metal adsorption isotherm and Cu (108 mg g(-1)) > Cd (54 mg g(-1)) ≥ Zn (44 mg g(-1)) in the ternary-metal adsorption isotherm. Cu was the most retained cation, whereas Zn could be easily exchanged and substituted by Cu. Batch experimental data best fit the Langmuir model rather than the Freundlich isotherms. In the column experiments, the total adsorbed amounts of the metals were in the following order of Cu (210 mg g(-1)) > Cd (192 mg g(-1)) > Zn (178) in single-metal conditions, and Cu (156) > Cd (123) > Zn (92) in ternary-metal conditions. Results from both the batch and column experiments indicate that competitive adsorption among metals increases the mobility of these metals. Especially, Zn in single-metal conditions lost it adsorption capacity most significantly. Based on the 3D simulation graphs of heavy metals, adsorption patterns under single adsorption condition were different than under competitive adsorption condition. Results from both the batch and column experiments show that competitive adsorption among metals increases the mobility of these metals. The maximum metal adsorption capacity of the metals in the column experiments was higher than that in the batch experiment indicating other metal retention mechanisms rather than adsorption may be involved. Therefore, both column and batch experiments are needed for estimating retention capacities and removal efficiencies of metals in CBB.

  18. Batch manufacturing: Six strategic needs

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Ash, R.H.; Chappell, D.A.

    1995-08-01

    Since the advent of industrial digital control systems in the mid-1970s, industry has had the promise of integrated, configurable digital batch control systems to replace the morass of electromechanical devices like relays and stepping switches, recorders, and indicators which comprised the components of previous generations of batch control systems - the {open_quotes}monolithic monsters{close_quotes} of the 1960s and earlier. To help fulfill that promise, there have been many wide-ranging proprietary automation solutions for batch control since 1975, many of them technically excellent. However, even the best examples suffered from the lack of a common language and unifying concept permitting separate systemsmore » to be interconnected and work together. Today, some 20 years after the digital revolution began, industry has microprocessors, memory chips, data highways, and other marvelous technology to help automate the control of discontinuous processes. They also are on the way to having an accepted standard for batch automation, ISA S88. Batching systems are at once conceptually simple but executionally complex. The notion of adding ingredients one at a time to a vat, mixing, and then processing into final form is as old as the stone age. Every homemaker on earth, male or female, is familiar with how to follow a recipe to create some sumptuous item of culinary delight. Food recipes, so familiar and ubiquitous, are really just microcosms of the S88 recipe standard. They contain the same components: (1) Header (name and description of item being prepared, sometimes serving size); (2) Formula (list and amount of ingredients); (3) Equipment requirements (pans, mixing and cooking equipment); (4) Procedure (description of order of ingredient addition, mixing and other processing steps, baking/cooling time, and other processing steps); and (5) Other information (safety, cautions, and other miscellaneous instructions).« less

  19. 40 CFR 80.75 - Reporting requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... section: (i) The batch number; (ii) The date of production; (iii) The volume of the batch; (iv) The grade... the refinery: (A) Identification of the previously certified gasoline as such; (B) The batch number... batch as commercial or non-commercial grade butane; (C) The batch number of the butane; (D) The date of...

  20. 27 CFR 19.748 - Dump/batch records.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Dump/batch records. 19.748... OF THE TREASURY LIQUORS DISTILLED SPIRITS PLANTS Records and Reports Processing Account § 19.748 Dump/batch records. (a) Format of dump/batch records. Proprietor's dump/batch records shall contain, as...

  1. A Model-based B2B (Batch to Batch) Control for An Industrial Batch Polymerization Process

    NASA Astrophysics Data System (ADS)

    Ogawa, Morimasa

    This paper describes overview of a model-based B2B (batch to batch) control for an industrial batch polymerization process. In order to control the reaction temperature precisely, several methods based on the rigorous process dynamics model are employed at all design stage of the B2B control, such as modeling and parameter estimation of the reaction kinetics which is one of the important part of the process dynamics model. The designed B2B control consists of the gain scheduled I-PD/II2-PD control (I-PD with double integral control), the feed-forward compensation at the batch start time, and the model adaptation utilizing the results of the last batch operation. Throughout the actual batch operations, the B2B control provides superior control performance compared with that of conventional control methods.

  2. Does Warming a Lysozyme Solution Cook Ones Data?

    NASA Technical Reports Server (NTRS)

    Pusey, Marc; Burke, Michael; Judge, Russell

    2000-01-01

    Chicken egg white lysozyme has a well characterized thermally driven phase transition. Between pH 4.0 and 5.2, the transition temperature, as defined by the point where the tetragonal and orthorhombic solubility are equal, is a function of the pH, salt (precipitant) type and concentration, and most likely of the buffer concentration as well. This phase transition can be carried out with protein solution alone, prior to the initiation of the crystallization process. We have now measured the kinetics of this process and investigated its reversibility. An aliquot of a stock protein solution is held at a given temperature, and at periodic intervals used to set up batch crystallization experiments. The batch solutions were incubated at 20 C until macroscopic crystals were obtained, at which point the number of crystals in each well were counted. The transition effects increased with temperature, slowly falling off at 30 C with a half time (time to approx. 1/2 the t = 0 number of crystals) of approx. 5 hours, and an estimated half time of approx. 0.5 hours at 43 C. Further, the process was not reversible by simple cooling. After holding a lysozyme solution at 37 C (prior to addition of precipitant) for 16 hours, then cooling and holding it at 4 C, no return to the pre-warmed nucleation kinetics are observed after at least 4 weeks. Thus every thermal excursion above the phase transition point results in a further decrease in the nucleation rate of that solution, the extent being a function of the time and temperature. Orthorhombic lysozyme crystals apparently do not undergo the flow-induced growth cessation of tetragonal lysozyme crystals. We have previously shown that putting the protein in the orthorhombic form does not affect the averaged face growth kinetics, only nucleation, for tetragonal crystals. We may be able to use this differential behavior to elucidate how flow affects tile lysozyme crystal growth process.

  3. Combined statistical analyses for long-term stability data with multiple storage conditions: a simulation study.

    PubMed

    Almalik, Osama; Nijhuis, Michiel B; van den Heuvel, Edwin R

    2014-01-01

    Shelf-life estimation usually requires that at least three registration batches are tested for stability at multiple storage conditions. The shelf-life estimates are often obtained by linear regression analysis per storage condition, an approach implicitly suggested by ICH guideline Q1E. A linear regression analysis combining all data from multiple storage conditions was recently proposed in the literature when variances are homogeneous across storage conditions. The combined analysis is expected to perform better than the separate analysis per storage condition, since pooling data would lead to an improved estimate of the variation and higher numbers of degrees of freedom, but this is not evident for shelf-life estimation. Indeed, the two approaches treat the observed initial batch results, the intercepts in the model, and poolability of batches differently, which may eliminate or reduce the expected advantage of the combined approach with respect to the separate approach. Therefore, a simulation study was performed to compare the distribution of simulated shelf-life estimates on several characteristics between the two approaches and to quantify the difference in shelf-life estimates. In general, the combined statistical analysis does estimate the true shelf life more consistently and precisely than the analysis per storage condition, but it did not outperform the separate analysis in all circumstances.

  4. Solvent-free enzymatic synthesis of 1, 3-Diacylglycerols by direct esterification of glycerol with saturated fatty acids

    PubMed Central

    2013-01-01

    Background Pure 1, 3-diacylglycerols (1, 3-DAG) have been considered to be significant surfactants in food, cosmetics and pharmaceutical industries, as well as the effect on obesity prevention. Methods In this study, a vacuum-driven air bubbling operation mode was developed and evaluated for the enzymatic synthesis of 1, 3-DAG of saturated fatty acids, by direct esterification of glycerol with fatty acids in a solvent-free system. The employed vacuum-driven air bubbling operation mode was comparable to vacuum-driven N2 bubbling protocol, in terms of lauric acid conversion and 1, 3-dilaurin content. Results Some operation parameters were optimized, and 95.3% of lauric acid conversion and 80.3% of 1, 3-dilaurin content was obtained after 3-h reaction at 50°C, with 5 wt% of Lipozyme RM IM (based on reactants) amount. Of the lipases studied, both Lipozyme RM IM and Novozym 435 exhibited good performance in terms of lauric acid conversion. Lipozyme TL IM, however, showed low activity. Lipozyme RM IM showed good operational stability in this operation protocol, 80.2% of the original catalytic activity remained after 10 consecutive batch applications. Some other 1, 3-DAG were prepared and high content was obtained after purification: 98.5% for 1, 3-dicaprylin, 99.2% for 1, 3-dicaprin, 99.1% for 1, 3-dilaurin, 99.5 for 1, 3-dipalmitin and 99.4% for 1, 3-disterin. Conclusion The established vacuum-driven air bubbling operation protocol had been demonstrated to be a simple-operating, cost-effective, application practical and efficient methodology for 1, 3-DAG preparation. PMID:23656739

  5. Box-and-Whisker Plots.

    ERIC Educational Resources Information Center

    Larsen, Russell D.

    1985-01-01

    Box-and-whisker plots (which give rapid visualization of batches of data) can be effectively used to present diverse collections of data used in traditional first-year chemistry courses. Construction of box-and-whisker plots and their use with bond energy data and data on heats of formation and solution are discussed. (JN)

  6. Graphite furnace atomic absorption elemental analysis of ecstasy tablets.

    PubMed

    French, Holly E; Went, Michael J; Gibson, Stuart J

    2013-09-10

    Six metals (copper, magnesium, barium, nickel, chromium and lead) were determined in two separate batches of seized ecstasy tablets by graphite furnace atomic absorption spectroscopy (GFAAS) following digestion with nitric acid and hydrogen peroxide. Large intra-batch variations were found as expected for tablets produced in clandestine laboratories. For example, nickel in batch 1 was present in the range 0.47-13.1 parts per million (ppm) and in batch 2 in the range 0.35-9.06 ppm. Although batch 1 had significantly higher 3,4-methylenedioxy-N-methamphetamine (MDMA) content than batch 2, barium was the only element which discriminated between the two ecstasy seizures (batch 1: 0.19-0.66 ppm, batch 2: 3.77-5.47 ppm). Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.

  7. Boosted structured additive regression for Escherichia coli fed-batch fermentation modeling.

    PubMed

    Melcher, Michael; Scharl, Theresa; Luchner, Markus; Striedner, Gerald; Leisch, Friedrich

    2017-02-01

    The quality of biopharmaceuticals and patients' safety are of highest priority and there are tremendous efforts to replace empirical production process designs by knowledge-based approaches. Main challenge in this context is that real-time access to process variables related to product quality and quantity is severely limited. To date comprehensive on- and offline monitoring platforms are used to generate process data sets that allow for development of mechanistic and/or data driven models for real-time prediction of these important quantities. Ultimate goal is to implement model based feed-back control loops that facilitate online control of product quality. In this contribution, we explore structured additive regression (STAR) models in combination with boosting as a variable selection tool for modeling the cell dry mass, product concentration, and optical density on the basis of online available process variables and two-dimensional fluorescence spectroscopic data. STAR models are powerful extensions of linear models allowing for inclusion of smooth effects or interactions between predictors. Boosting constructs the final model in a stepwise manner and provides a variable importance measure via predictor selection frequencies. Our results show that the cell dry mass can be modeled with a relative error of about ±3%, the optical density with ±6%, the soluble protein with ±16%, and the insoluble product with an accuracy of ±12%. Biotechnol. Bioeng. 2017;114: 321-334. © 2016 Wiley Periodicals, Inc. © 2016 Wiley Periodicals, Inc.

  8. Navigation Operations with Prototype Components of an Automated Real-Time Spacecraft Navigation System

    NASA Technical Reports Server (NTRS)

    Cangahuala, L.; Drain, T. R.

    1999-01-01

    At present, ground navigation support for interplanetary spacecraft requires human intervention for data pre-processing, filtering, and post-processing activities; these actions must be repeated each time a new batch of data is collected by the ground data system.

  9. Effect of mineral surface properties (alumina, kaolinite) on the sorptive fractionation mechanisms of soil fulvic acids: Molecular-scale ESI-MS studies

    NASA Astrophysics Data System (ADS)

    Fleury, Guillaume; Del Nero, Mirella; Barillon, Rémi

    2017-01-01

    We addressed the effects of mineral surface properties (kaolinite versus Al-oxide) on the sorption-driven fractionation of a soil fulvic acid (FA) at acidic pH, mainly by means of ESI(-)-FTMS analysis of initial and supernatant solutions of FA sorption batch experiments. The MS data provided clear molecular-scale evidence of distinct mechanisms and molecular parameters controlling the FA fractionation upon its sorption on clay and oxide surfaces, respectively. Identification of sorbing and not-sorbing FA compounds in kaolinite-solution systems revealed a weak fractionation among members of sbnd CO2 series of aliphatics or not-condensed aromatics (NCAs) at pH 3.8, and almost no sorption of poorly-oxygenated polycyclic aromatic compounds (PACs) and NCAs. This first molecular-scale description of a FA fractionation in a clay-solution system suggests that H-bonding with low affinity sites (aluminol/silanol) on the basal planes of the clay particles is the main mechanism of sorption. Due to the predominance of such weak and poorly-selective mechanism, the sorption of aliphatic and NCA molecules bearing oxygenated functionalities was prevented at pH 5, due to dissolved Al competing successfully for their coordination. In contrast, a strong FA fractionation was observed onto alumina, with a preferential retention of PACs and highly-oxygenated aliphatics and NCAs. The major part of the poorly oxygenated aliphatics was left in solution. The sorption degree of NCAs and aliphatics was strongly correlated with molecular acidity. For PACs and poorly-oxygenated NCAs, the sorption was driven by reactions of surface ligand exchange (for the most oxygenated compounds) or by hydrophobic interactions (for the least oxygenated compounds).

  10. Traditionally used medicinal plants against uncomplicated urinary tract infections: Are unusual, flavan-4-ol- and derhamnosylmaysin derivatives responsible for the antiadhesive activity of extracts obtained from stigmata of Zea mays L. against uropathogenic E. coli and Benzethonium chloride as frequent contaminant faking potential antibacterial activities?

    PubMed

    Rafsanjany, Nasli; Sendker, Jandirk; Lechtenberg, Matthias; Petereit, Frank; Scharf, Birte; Hensel, Andreas

    2015-09-01

    The dried stigmata from Zea mays L. are used traditionally for the treatment of uncomplicated urinary tract infections. A recent screening has indicated that hydroalcoholic extract of the herbal material inhibits the adhesion of uropathogenic Escherichia coli (UPEC) to T24 bladder cells. For verification of these data EtOH-water (1:1) extracts from 4 different batches of Maydis stigmata were investigated. Within an in vitro adhesion assay (UPEC strain 2980 and human T24 bladder cells) a dose-dependent antiadhesive activity against UPEC was verified (IC50 1040μg/mL). Bioassay guided fractionation of M. stigmata, batch S1, by EtOH-water extraction, followed by chromatography on Sephadex LH20 revealed two active fractions (I and XI). Further purification of fraction I and structure elucidation of the isolated compound revealed the presence of significant amounts of the biocide benzethonium chloride as contaminant. Benzethonium chloride was also identified in subsequent investigations in 2 different batches of M. stigmata. The presence of such nondeclared and illegal contaminants in the herbal raw material market has to be discussed intensively. From benzethonium-free raw material (batch S2) as well as from batch S1 fraction XI was further fractionated by MPLC and preparative HPLC, leading to a still complex subfraction XIG, which was analyzed by UHPLC/+ESI-QTOF-MS analysis. Advanced data processing and species-metabolite relationship database revealed the tentatively existence of the unusual C-glycosidic flavones derhamnosylmaysin (6), 3'-deoxyrhamnosylmaysin (4), 3'-O-methylderhamnosylmaysin (3), apiferol (2) and alternanthin (8) which might be related to the antiadhesive activity of this subfraction against UPEC. Copyright © 2015 Elsevier B.V. All rights reserved.

  11. Commercial antibodies and their validation

    PubMed Central

    Voskuil, JLA

    2014-01-01

    Despite an impressive growth in the business of research antibodies a general lack of trust in commercial antibodies remains in place. A variety of issues, each one potentially causing an antibody to fail, underpin the frustrations that scientists endure. Lots of money goes to waste in buying and trying one failing antibody after the other without realizing all the pitfalls that come with the product: Antibodies can get inactivated, both the biological material and the assay itself can potentially be flawed, a single antibody featuring in many different catalogues can be deemed as a set of different products, and a bad choice of antibody type, wrong dilutions, and lack of proper validation can all jeopardize the intended experiments. Antibodies endorsed by scientific research papers do not always meet the scientist’s requirements either due to flawed specifications, or due to batch-to-batch variations. Antibodies can be found with Quality Control data obtained from previous batches that no longer represent the batch on sale. In addition, one cannot assume that every antibody is fit for every application. The best chance of success is to try an antibody that already was confirmed to perform correctly in the required platform. PMID:25324967

  12. Pollution prevention applications in batch manufacturing operations

    NASA Astrophysics Data System (ADS)

    Sykes, Derek W.; O'Shaughnessy, James

    2004-02-01

    Older, "low-tech" batch manufacturing operations are often fertile grounds for gains resulting from pollution prevention techniques. This paper presents a pollution prevention technique utilized for wastewater discharge permit compliance purposes at a batch manufacturer of detergents, deodorants, and floor-care products. This manufacturer generated industrial wastewater as a result of equipment rinses required after each product batch changeover. After investing a significant amount of capital on end of pip-line wastewater treatment technology designed to address existing discharge limits, this manufacturer chose to investigate alternate, low-cost approaches to address anticipated new permit limits. Mass balances using spreadsheets and readily available formulation and production data were conducted on over 300 products to determine how each individual product contributed to the total wastewater pollutant load. These mass balances indicated that 22 products accounted for over 55% of the wastewater pollutant. Laboratory tests were conducted to determine whether these same products could accept their individual changeover rinse water as make-up water in formulations without sacrificing product quality. This changeover reuse technique was then implement at the plant scale for selected products. Significant reductions in wastewater volume (25%) and wastewater pollutant loading (85+%) were realized as a direct result of this approach.

  13. Biodegradation kinetics of 1,4-benzoquinone in batch and continuous systems.

    PubMed

    Kumar, Pardeep; Nemati, Mehdi; Hill, Gordon A

    2011-11-01

    Combining chemical and biological treatments is a potentially economic approach to remove high concentration of recalcitrant compounds from wastewaters. In the present study, the biodegradation of 1,4-benzoquinone, an intermediate compound formed during phenol oxidation by chlorine dioxide, was investigated using Pseudomonas putida (ATCC 17484) in batch and continuous bioreactors. Batch experiments were conducted to determine the effects of 1,4-benzoquinone concentration and temperature on the microbial activity and biodegradation kinetics. Using the generated data, the maximum specific growth rate and biodegradation rate were determined as 0.94 h(-1) and 6.71 mg of 1,4-benzoquinone l(-1) h(-1). Biodegradation in a continuous bioreactor indicated a linear relationship between substrate loading and biodegradation rates prior to wash out of the cells, with a maximum biodegradation rate of 246 mg l(-1) h(-1) observed at a loading rate of 275 mg l(-1) h(-1) (residence time: 1.82 h). Biokinetic parameters were also determined using the steady state substrate and biomass concentrations at various dilution rates and compared to those obtained in batch cultures.

  14. Near infrared spectroscopy based monitoring of extraction processes of raw material with the help of dynamic predictive modeling

    NASA Astrophysics Data System (ADS)

    Wang, Haixia; Suo, Tongchuan; Wu, Xiaolin; Zhang, Yue; Wang, Chunhua; Yu, Heshui; Li, Zheng

    2018-03-01

    The control of batch-to-batch quality variations remains a challenging task for pharmaceutical industries, e.g., traditional Chinese medicine (TCM) manufacturing. One difficult problem is to produce pharmaceutical products with consistent quality from raw material of large quality variations. In this paper, an integrated methodology combining the near infrared spectroscopy (NIRS) and dynamic predictive modeling is developed for the monitoring and control of the batch extraction process of licorice. With the spectra data in hand, the initial state of the process is firstly estimated with a state-space model to construct a process monitoring strategy for the early detection of variations induced by the initial process inputs such as raw materials. Secondly, the quality property of the end product is predicted at the mid-course during the extraction process with a partial least squares (PLS) model. The batch-end-time (BET) is then adjusted accordingly to minimize the quality variations. In conclusion, our study shows that with the help of the dynamic predictive modeling, NIRS can offer the past and future information of the process, which enables more accurate monitoring and control of process performance and product quality.

  15. Peregrine Job Queues and Scheduling Policies | High-Performance Computing |

    Science.gov Websites

    batch batch-h long bigmem data-transfer feature Max wall time 1 hour 4 hours 2 days 2 days 10 days 10 # nodes per job 2 8 288 576 120 46 1 # of 24 core 64 GB Haswell nodes 2 8 0 1228 0 0 0 haswell # of 24core 32 GB nodes 2 16 576 0 126 0 0 24core # of 16core 32 GB nodes 2 8 195 0 162 0 5 16core, # of 24core

  16. Investigation of vinegar production using a novel shaken repeated batch culture system.

    PubMed

    Schlepütz, Tino; Büchs, Jochen

    2013-01-01

    Nowadays, bioprocesses are developed or optimized on small scale. Also, vinegar industry is motivated to reinvestigate the established repeated batch fermentation process. As yet, there is no small-scale culture system for optimizing fermentation conditions for repeated batch bioprocesses. Thus, the aim of this study is to propose a new shaken culture system for parallel repeated batch vinegar fermentation. A new operation mode - the flushing repeated batch - was developed. Parallel repeated batch vinegar production could be established in shaken overflow vessels in a completely automated operation with only one pump per vessel. This flushing repeated batch was first theoretically investigated and then empirically tested. The ethanol concentration was online monitored during repeated batch fermentation by semiconductor gas sensors. It was shown that the switch from one ethanol substrate quality to different ethanol substrate qualities resulted in prolonged lag phases and durations of the first batches. In the subsequent batches the length of the fermentations decreased considerably. This decrease in the respective lag phases indicates an adaptation of the acetic acid bacteria mixed culture to the specific ethanol substrate quality. Consequently, flushing repeated batch fermentations on small scale are valuable for screening fermentation conditions and, thereby, improving industrial-scale bioprocesses such as vinegar production in terms of process robustness, stability, and productivity. Copyright © 2013 American Institute of Chemical Engineers.

  17. Multivariate data analysis on historical IPV production data for better process understanding and future improvements.

    PubMed

    Thomassen, Yvonne E; van Sprang, Eric N M; van der Pol, Leo A; Bakker, Wilfried A M

    2010-09-01

    Historical manufacturing data can potentially harbor a wealth of information for process optimization and enhancement of efficiency and robustness. To extract useful data multivariate data analysis (MVDA) using projection methods is often applied. In this contribution, the results obtained from applying MVDA on data from inactivated polio vaccine (IPV) production runs are described. Data from over 50 batches at two different production scales (700-L and 1,500-L) were available. The explorative analysis performed on single unit operations indicated consistent manufacturing. Known outliers (e.g., rejected batches) were identified using principal component analysis (PCA). The source of operational variation was pinpointed to variation of input such as media. Other relevant process parameters were in control and, using this manufacturing data, could not be correlated to product quality attributes. The gained knowledge of the IPV production process, not only from the MVDA, but also from digitalizing the available historical data, has proven to be useful for troubleshooting, understanding limitations of available data and seeing the opportunity for improvements. 2010 Wiley Periodicals, Inc.

  18. Calcium transcriptionally regulates the biofilm machinery of Xylella fastidiosa to promote continued biofilm development in batch cultures.

    PubMed

    Parker, Jennifer K; Chen, Hongyu; McCarty, Sara E; Liu, Lawrence Y; De La Fuente, Leonardo

    2016-05-01

    The functions of calcium (Ca) in bacteria are less characterized than in eukaryotes, where its role has been studied extensively. The plant-pathogenic bacterium Xylella fastidiosa has several virulence features that are enhanced by increased Ca concentrations, including biofilm formation. However, the specific mechanisms driving modulation of this feature are unclear. Characterization of biofilm formation over time showed that 4 mM Ca supplementation produced denser biofilms that were still developing at 96 h, while biofilm in non-supplemented media had reached the dispersal stage by 72 h. To identify changes in global gene expression in X. fastidiosa grown in supplemental Ca, RNA-Seq of batch culture biofilm cells was conducted at three 24-h time intervals. Results indicate that a variety of genes are differentially expressed in response to Ca, including genes related to attachment, motility, exopolysaccharide synthesis, biofilm formation, peptidoglycan synthesis, regulatory functions, iron homeostasis, and phages. Collectively, results demonstrate that Ca supplementation induces a transcriptional response that promotes continued biofilm development, while biofilm cells in nonsupplemented media are driven towards dispersion of cells from the biofilm structure. These results have important implications for disease progression in planta, where xylem sap is the source of Ca and other nutrients for X. fastidiosa. © 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.

  19. Bioaggregate of photo-fermentative bacteria for enhancing continuous hydrogen production in a sequencing batch photobioreactor

    NASA Astrophysics Data System (ADS)

    Xie, Guo-Jun; Liu, Bing-Feng; Wang, Rui-Qing; Ding, Jie; Ren, Hong-Yu; Zhou, Xu; Ren, Nan-Qi

    2015-11-01

    Hydrogen recovery through solar-driven biomass conversion by photo-fermentative bacteria (PFB) has been regarded as a promising way for sustainable energy production. However, a considerable fraction of organic substrate was consumed for the growth of PFB as biocatalysts, furthermore, these PFB were continuously washed out from the photobioreactor in continuous operation because of their poor flocculation. In this work, PFB bioaggregate induced by L-cysteine was applied in a sequencing batch photobioreactor to enhance continuous hydrogen production and reduce biomass washout. The effects of the hydraulic retention time (HRT), influent concentration and light intensity on hydrogen production of the photobioreactor were investigated. The maximum hydrogen yield (3.35 mol H2/mol acetate) and production rate (1044 ml/l/d) were obtained at the HRT of 96 h, influent concentration of 3.84 g COD/l, and light intensity of 200 W/m2. With excellent settling ability, biomass accumulated in the photobioreactor and reached 2.15 g/l under the optimum conditions. Structural analysis of bioaggregate showed that bacterial cells were covered and tightly linked together by extracellular polymeric substances, and formed a stable structure. Therefore, PFB bioaggregate induced by L-cysteine is an efficient strategy to improve biomass retention capacity of the photobioreactor and enhance hydrogen recovery efficiency from organic wastes.

  20. Modelling the anaerobic digestion of solid organic waste - Substrate characterisation method for ADM1 using a combined biochemical and kinetic parameter estimation approach.

    PubMed

    Poggio, D; Walker, M; Nimmo, W; Ma, L; Pourkashanian, M

    2016-07-01

    This work proposes a novel and rigorous substrate characterisation methodology to be used with ADM1 to simulate the anaerobic digestion of solid organic waste. The proposed method uses data from both direct substrate analysis and the methane production from laboratory scale anaerobic digestion experiments and involves assessment of four substrate fractionation models. The models partition the organic matter into a mixture of particulate and soluble fractions with the decision on the most suitable model being made on quality of fit between experimental and simulated data and the uncertainty of the calibrated parameters. The method was tested using samples of domestic green and food waste and using experimental data from both short batch tests and longer semi-continuous trials. The results showed that in general an increased fractionation model complexity led to better fit but with increased uncertainty. When using batch test data the most suitable model for green waste included one particulate and one soluble fraction, whereas for food waste two particulate fractions were needed. With richer semi-continuous datasets, the parameter estimation resulted in less uncertainty therefore allowing the description of the substrate with a more complex model. The resulting substrate characterisations and fractionation models obtained from batch test data, for both waste samples, were used to validate the method using semi-continuous experimental data and showed good prediction of methane production, biogas composition, total and volatile solids, ammonia and alkalinity. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

  1. Cometabolic degradation kinetics of TCE and phenol by Pseudomonas putida.

    PubMed

    Chen, Yan-Min; Lin, Tsair-Fuh; Huang, Chih; Lin, Jui-Che

    2008-08-01

    Modeling of cometabolic kinetics is important for better understanding of degradation reaction and in situ application of bio-remediation. In this study, a model incorporated cell growth and decay, loss of transformation activity, competitive inhibition between growth substrate and non-growth substrate and self-inhibition of non-growth substrate was proposed to simulate the degradation kinetics of phenol and trichloroethylene (TCE) by Pseudomonas putida. All the intrinsic parameters employed in this study were measured independently, and were then used for predicting the batch experimental data. The model predictions conformed well to the observed data at different phenol and TCE concentrations. At low TCE concentrations (<2 mg l(-1)), the models with or without self-inhibition of non-growth substrate both simulated the experimental data well. However, at higher TCE concentrations (>6 mg l(-1)), only the model considering self-inhibition can describe the experimental data, suggesting that a self-inhibition of TCE was present in the system. The proposed model was also employed in predicting the experimental data conducted in a repeated batch reactor, and good agreements were observed between model predictions and experimental data. The results also indicated that the biomass loss in the degradation of TCE below 2 mg l(-1) can be totally recovered in the absence of TCE for the next cycle, and it could be used for the next batch experiment for the degradation of phenol and TCE. However, for higher concentration of TCE (>6 mg l(-1)), the recovery of biomass may not be as good as that at lower TCE concentrations.

  2. Batch Scheduling for Hybrid Assembly Differentiation Flow Shop to Minimize Total Actual Flow Time

    NASA Astrophysics Data System (ADS)

    Maulidya, R.; Suprayogi; Wangsaputra, R.; Halim, A. H.

    2018-03-01

    A hybrid assembly differentiation flow shop is a three-stage flow shop consisting of Machining, Assembly and Differentiation Stages and producing different types of products. In the machining stage, parts are processed in batches on different (unrelated) machines. In the assembly stage, each part of the different parts is assembled into an assembly product. Finally, the assembled products will further be processed into different types of final products in the differentiation stage. In this paper, we develop a batch scheduling model for a hybrid assembly differentiation flow shop to minimize the total actual flow time defined as the total times part spent in the shop floor from the arrival times until its due date. We also proposed a heuristic algorithm for solving the problems. The proposed algorithm is tested using a set of hypothetic data. The solution shows that the algorithm can solve the problems effectively.

  3. Ion exchange of several radionuclides on the hydrous crystalline silicotitanate, UOP IONSIV IE-911

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Huckman, M.E.; Latheef, I.M.; Anthony, R.G.

    1999-04-01

    The crystalline silicotitanate, UOP IONSIV IE-911, is a proven material for removing radionuclides from a wide variety of waste streams. It is superior for removing several radionuclides from the highly alkaline solutions typical of DOE wastes. This laboratory previously developed an equilibrium model applicable to complex solutions for IE-910 (the power form of the granular IE-911), and more recently, the authors have developed several single component ion-exchange kinetic models for predicting column breakthrough curves and batch reactor concentration histories. In this paper, the authors model ion-exchange column performance using effective diffusivities determined from batch kinetic experiments. This technique is preferablemore » because the batch experiments are easier, faster, and cheaper to perform than column experiments. They also extend these ideas to multicomponent systems. Finally, they evaluate the ability of the equilibrium model to predict data for IE-911.« less

  4. Analytical solution of Luedeking-Piret equation for a batch fermentation obeying Monod growth kinetics.

    PubMed

    Garnier, Alain; Gaillet, Bruno

    2015-12-01

    Not so many fermentation mathematical models allow analytical solutions of batch process dynamics. The most widely used is the combination of the logistic microbial growth kinetics with Luedeking-Piret bioproduct synthesis relation. However, the logistic equation is principally based on formalistic similarities and only fits a limited range of fermentation types. In this article, we have developed an analytical solution for the combination of Monod growth kinetics with Luedeking-Piret relation, which can be identified by linear regression and used to simulate batch fermentation evolution. Two classical examples are used to show the quality of fit and the simplicity of the method proposed. A solution for the combination of Haldane substrate-limited growth model combined with Luedeking-Piret relation is also provided. These models could prove useful for the analysis of fermentation data in industry as well as academia. © 2015 Wiley Periodicals, Inc.

  5. Prediction of acid hydrolysis of lignocellulosic materials in batch and plug flow reactors.

    PubMed

    Jaramillo, Oscar Johnny; Gómez-García, Miguel Ángel; Fontalvo, Javier

    2013-08-01

    This study unifies contradictory conclusions reported in literature on acid hydrolysis of lignocellulosic materials, using batch and plug flow reactors, regarding the influence of the initial liquid ratio of acid aqueous solution to solid lignocellulosic material on sugar yield and concentration. The proposed model takes into account the volume change of the reaction media during the hydrolysis process. An error lower than 8% was found between predictions, using a single set of kinetic parameters for several liquid to solid ratios, and reported experimental data for batch and plug flow reactors. For low liquid-solid ratios, the poor wetting and the acid neutralization, due to the ash presented in the solid, will both reduce the sugar yield. Also, this study shows that both reactors are basically equivalent in terms of the influence of the liquid to solid ratio on xylose and glucose yield. Copyright © 2013 Elsevier Ltd. All rights reserved.

  6. Fed-batch coculture of Lactobacillus kefiranofaciens with Saccharomyces cerevisiae for effective production of kefiran.

    PubMed

    Tada, Shiori; Katakura, Yoshio; Ninomiya, Kazuaki; Shioya, Suteaki

    2007-06-01

    In a batch coculture of kefiran-producing lactic acid bacteria Lactobacillus kefiranofaciens and lactate-assimilating yeast Saccharomyces cerevisiae, lactate accumulation in the medium was observed, which inhibited kefiran production. To enhance kefiran productivity by preventing lactate accumulation, we conducted lactose-feeding batch operation with feedforward/feedback control during the coculture, so that the lactate production rate of L. kefiranofaciens was balanced with the lactate consumption rate of S. cerevisiae. The lactate concentration was maintained at less than 6 g l(-1) throughout the fed-batch coculture using a 5 l jar fermentor, although the concentration reached 33 g l(-1) in the batch coculture. Kefiran production was increased to 6.3 g in 102 h in the fed-batch coculture, whereas 4.5 g kefiran was produced in 97 h in the batch coculture. The kefiran yield on lactose basis was increased up to 0.033 g g(-1) in the fed-batch coculture, whereas that in the batch coculture was 0.027 g g(-1).

  7. Real-time imaging as an emerging process analytical technology tool for monitoring of fluid bed coating process.

    PubMed

    Naidu, Venkata Ramana; Deshpande, Rucha S; Syed, Moinuddin R; Wakte, Pravin S

    2018-07-01

    A direct imaging system (Eyecon TM ) was used as a Process Analytical Technology (PAT) tool to monitor fluid bed coating process. Eyecon TM generated real-time onscreen images, particle size and shape information of two identically manufactured laboratory-scale batches. Eyecon TM has accuracy of measuring the particle size increase of ±1 μm on particles in the size range of 50-3000 μm. Eyecon TM captured data every 2 s during the entire process. The moving average of D90 particle size values recorded by Eyecon TM were calculated for every 30 min to calculate the radial coating thickness of coated particles. After the completion of coating process, the radial coating thickness was found to be 11.3 and 9.11 μm, with a standard deviation of ±0.68 and 1.8 μm for Batch 1 and Batch 2, respectively. The coating thickness was also correlated with percent weight build-up by gel permeation chromatography (GPC) and dissolution. GPC indicated weight build-up of 10.6% and 9.27% for Batch 1 and Batch 2, respectively. In conclusion, weight build-up of 10% can also be correlated with 10 ± 2 μm increase in the coating thickness of pellets, indicating the potential applicability of real-time imaging as an endpoint determination tool for fluid bed coating process.

  8. Modeling of the adsorptive removal of arsenic(III) using plant biomass: a bioremedial approach

    NASA Astrophysics Data System (ADS)

    Roy, Palas; Dey, Uttiya; Chattoraj, Soumya; Mukhopadhyay, Debasis; Mondal, Naba Kumar

    2017-06-01

    In the present work, the possibility of using a non-conventional finely ground (250 μm) Azadirachta indica (neem) bark powder [AiBP] has been tested as a low-cost biosorbent for the removal of arsenic(III) from water. The removal of As(III) was studied by performing a series of biosorption experiments (batch and column). The biosorption behavior of As(III) for batch and column operations were examined in the concentration ranges of 50-500 µg L-1 and 500.0-2000.0 µg L-1, respectively. Under optimized batch conditions, the AiBP could remove up to 89.96 % of As(III) in water system. The artificial neural network (ANN) model was developed from batch experimental data sets which provided reasonable predictive performance ( R 2 = 0.961; 0.954) of As(III) biosorption. In batch operation, the initial As(III) concentration had the most significant impact on the biosorption process. For column operation, central composite design (CCD) was applied to investigate the influence on the breakthrough time for optimization of As(III) biosorption process and evaluation of interacting effects of different operating variables. The optimized result of CCD revealed that the AiBP was an effective and economically feasible biosorbent with maximum breakthrough time of 653.9 min, when the independent variables were retained at 2.0 g AiBP dose, 2000.0 µg L-1 initial As(III) concentrations, and 3.0 mL min-1 flow rate, at maximum desirability value of 0.969.

  9. Supporting the human life-raft in confronting the juggernaut of technology: Jens Rasmussen, 1961-1986.

    PubMed

    Kant, Vivek

    2017-03-01

    Jens Rasmussen's contribution to the field of human factors and ergonomics has had a lasting impact. Six prominent interrelated themes can be extracted from his research between 1961 and 1986. These themes form the basis of an engineering epistemology which is best manifested by his abstraction hierarchy. Further, Rasmussen reformulated technical reliability using systems language to enable a proper human-machine fit. To understand the concept of human-machine fit, he included the operator as a central component in the system to enhance system safety. This change resulted in the application of a qualitative and categorical approach for human-machine interaction design. Finally, Rasmussen's insistence on a working philosophy of systems design as being a joint responsibility of operators and designers provided the basis for averting errors and ensuring safe and correct system functioning. Copyright © 2016 Elsevier Ltd. All rights reserved.

  10. Modeling hexavalent chromium reduction in groundwater in field-scale transport and laboratory batch experiments

    USGS Publications Warehouse

    Friedly, J.C.; Davis, J.A.; Kent, D.B.

    1995-01-01

    A plausible and consistent model is developed to obtain a quantitative description of the gradual disappearance of hexavalent chromium (Cr(VI)) from groundwater in a small-scale field tracer test and in batch kinetic experiments using aquifer sediments under similar chemical conditions. The data exhibit three distinct timescales. Fast reduction occurs in well-stirred batch reactors in times much less than 1 hour and is followed by slow reduction over a timescale of the order of 2 days. In the field, reduction occurs on a timescale of the order of 8 days. The model is based on the following hypotheses. The chemical reduction reaction occurs very fast, and the longer timescales are caused by diffusion resistance. Diffusion into the secondary porosity of grains causes the apparent slow reduction rate in batch experiments. In the model of the field experiments, the reducing agent, heavy Fe(II)-bearing minerals, is heterogeneously distributed in thin strata located between larger nonreducing sand lenses that comprise the bulk of the aquifer solids. It is found that reducing strata of the order of centimeters thick are sufficient to contribute enough diffusion resistance to cause the observed longest timescale in the field. A one-dimensional advection/dispersion model is formulated that describes the major experimental trends. Diffusion rates are estimated in terms of an elementary physical picture of flow through a stratified medium containing identically sized spherical grains. Both reduction and sorption reactions are included. Batch simulation results are sensitive to the fraction of reductant located at or near the surface of grains, which controls the amount of rapid reduction, and the secondary porosity, which controls the rate of slow reduction observed in batch experiments. Results of Cr(VI) transport simulations are sensitive to the thickness and relative size of the reducing stratum. Transport simulation results suggest that nearly all of the reductant must be located in the reducing stratum. Within this context and as long as there is adequate reductive capacity present, the transport simulation results are insensitive to the parameters important for the batch simulations. The results illustrate how a combination of field measurements and batch laboratory studies can be used to improve predictive modeling of contaminant transport.

  11. SLUDGE WASHING AND DEMONSTRATION OF THE DWPF FLOWSHEET IN THE SRNL SHIELDED CELLS FOR SLUDGE BATCH 7A QUALIFICATION

    DOE Office of Scientific and Technical Information (OSTI.GOV)

    Pareizs, J.; Billings, A.; Click, D.

    2011-07-08

    Waste Solidification Engineering (WSE) has requested that characterization and a radioactive demonstration of the next batch of sludge slurry (Sludge Batch 7a*) be completed in the Shielded Cells Facility of the Savannah River National Laboratory (SRNL) via a Technical Task Request (TTR). This characterization and demonstration, or sludge batch qualification process, is required prior to transfer of the sludge from Tank 51 to the Defense Waste Processing Facility (DWPF) feed tank (Tank 40). The current WSE practice is to prepare sludge batches in Tank 51 by transferring sludge from other tanks. Discharges of nuclear materials from H Canyon are oftenmore » added to Tank 51 during sludge batch preparation. The sludge is washed and transferred to Tank 40, the current DWPF feed tank. Prior to transfer of Tank 51 to Tank 40, SRNL simulates the Tank Farm and DWPF processes with a Tank 51 sample (referred to as the qualification sample). Sludge Batch 7a (SB7a) is composed of portions of Tanks 4, 7, and 12; the Sludge Batch 6 heel in Tank 51; and a plutonium stream from H Canyon. SRNL received the Tank 51 qualification sample (sample ID HTF-51-10-125) following sludge additions to Tank 51. This report documents: (1) The washing (addition of water to dilute the sludge supernate) and concentration (decanting of supernate) of the SB7a - Tank 51 qualification sample to adjust sodium content and weight percent insoluble solids to Tank Farm projections. (2) The performance of a DWPF Chemical Process Cell (CPC) simulation using the washed Tank 51 sample. The simulation included a Sludge Receipt and Adjustment Tank (SRAT) cycle, where acid was added to the sludge to destroy nitrite and reduce mercury, and a Slurry Mix Evaporator (SME) cycle, where glass frit was added to the sludge in preparation for vitrification. The SME cycle also included replication of five canister decontamination additions and concentrations. Processing parameters were based on work with a non-radioactive simulant. (3) Vitrification of a portion of the SME product and characterization and durability testing (as measured by the Product Consistency Test (PCT)) of the resulting glass. (4) Rheology measurements of the initial slurry samples and samples after each phase of CPC processing. This program was controlled by a Task Technical and Quality Assurance Plan (TTQAP), and analyses were guided by an Analytical Study Plan. This work is Technical Baseline Research and Development (R&D) for the DWPF. It should be noted that much of the data in this document has been published in interoffice memoranda. The intent of this technical report is bring all of the SB7a related data together in a single permanent record and to discuss the overall aspects of SB7a processing.« less

  12. 27 CFR 19.598 - Dump/batch records.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... ingredients used; (j) Formula number; (k) Quantity of ingredients used in the batch that have been previously... product transferred; (q) Batch gain or loss; and (r) For each batch to be tax determined in accordance...

  13. 27 CFR 19.598 - Dump/batch records.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... ingredients used; (j) Formula number; (k) Quantity of ingredients used in the batch that have been previously... product transferred; (q) Batch gain or loss; and (r) For each batch to be tax determined in accordance...

  14. 27 CFR 19.598 - Dump/batch records.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... ingredients used; (j) Formula number; (k) Quantity of ingredients used in the batch that have been previously... product transferred; (q) Batch gain or loss; and (r) For each batch to be tax determined in accordance...

  15. 27 CFR 19.598 - Dump/batch records.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... ingredients used; (j) Formula number; (k) Quantity of ingredients used in the batch that have been previously... product transferred; (q) Batch gain or loss; and (r) For each batch to be tax determined in accordance...

  16. Driving Ms. Data: Creating Data-Driven Possibilities

    ERIC Educational Resources Information Center

    Hoffman, Richard

    2005-01-01

    This article describes how driven Web sites help schools and districts maximize their IT resources by making online content more "self-service" for users. It shows how to set up the capacity to create data-driven sites. By definition, a data-driven Web site is one in which the content comes from some back-end data source, such as a…

  17. GridPP - Preparing for LHC Run 2 and the Wider Context

    NASA Astrophysics Data System (ADS)

    Coles, Jeremy

    2015-12-01

    This paper elaborates upon the operational status and directions within the UK Computing for Particle Physics (GridPP) project as it approaches LHC Run 2. It details the pressures that have been gradually reshaping the deployed hardware and middleware environments at GridPP sites - from the increasing adoption of larger multicore nodes to the move towards alternative batch systems and cloud alternatives - as well as changes being driven by funding considerations. The paper highlights work being done with non-LHC communities and describes some of the early outcomes of adopting a generic DIRAC based job submission and management framework. The paper presents results from an analysis of how GridPP effort is distributed across various deployment and operations tasks and how this may be used to target further improvements in efficiency.

  18. Teaching the Teachers: Physical Science for the Non-Scientific

    NASA Astrophysics Data System (ADS)

    Michels, D. J.; Pickert, S. M.; Montrose, C. J.; Thompson, J. L.

    2004-12-01

    The Catholic University of America, in collaboration with the Solar Physics Branch of the Naval Research Laboratory and the Goddard Space Flight Center, has begun development of an experimental, inquiry-driven and standards-referenced physical science course for undergraduate, pre-service K-8 teachers. The course is team-taught by faculty from the University's Departments of Education and Physics and NRL solar physics research personnel. Basic physical science concepts are taught in the context of the Sun and Sun-Earth Connections, through direct observation, web-based solar data, and images and movies from ongoing space missions. The Sun can illuminate, in ways that cannot be duplicated with comparable clarity in the laboratory, the basics of magnetic and gravitational force fields, Newton's Laws, and light and optics. The immediacy of the connection to ongoing space research and live mission data serves as well to inspire student interest and curiosity. Teaching objectives include pedagogical methods, especially hands-on and observational experiences appropriate to the physics content and the K-8 classroom. The CUA Program, called TOPS! (Top Teachers of Physical Science!) has completed its first year of classroom experience; the first few batches of Program graduates should be in K-8 classrooms in time to capitalize on the motivational opportunities offered by the 2007-2008 IHY and IPY. We present data on the attitudinal and scientific progress of fifteen pre-service Early Childhood and Elementary Education majors as they experienced, many for the first time, the marvels of attractive and repulsive forces, live observations of solar system dynamics, access to real-time satellite data and NASA educational resources.

  19. MethLAB: a graphical user interface package for the analysis of array-based DNA methylation data.

    PubMed

    Kilaru, Varun; Barfield, Richard T; Schroeder, James W; Smith, Alicia K; Conneely, Karen N

    2012-03-01

    Recent evidence suggests that DNA methylation changes may underlie numerous complex traits and diseases. The advent of commercial, array-based methods to interrogate DNA methylation has led to a profusion of epigenetic studies in the literature. Array-based methods, such as the popular Illumina GoldenGate and Infinium platforms, estimate the proportion of DNA methylated at single-base resolution for thousands of CpG sites across the genome. These arrays generate enormous amounts of data, but few software resources exist for efficient and flexible analysis of these data. We developed a software package called MethLAB (http://genetics.emory.edu/conneely/MethLAB) using R, an open source statistical language that can be edited to suit the needs of the user. MethLAB features a graphical user interface (GUI) with a menu-driven format designed to efficiently read in and manipulate array-based methylation data in a user-friendly manner. MethLAB tests for association between methylation and relevant phenotypes by fitting a separate linear model for each CpG site. These models can incorporate both continuous and categorical phenotypes and covariates, as well as fixed or random batch or chip effects. MethLAB accounts for multiple testing by controlling the false discovery rate (FDR) at a user-specified level. Standard output includes a spreadsheet-ready text file and an array of publication-quality figures. Considering the growing interest in and availability of DNA methylation data, there is a great need for user-friendly open source analytical tools. With MethLAB, we present a timely resource that will allow users with no programming experience to implement flexible and powerful analyses of DNA methylation data.

  20. DETAIL VIEW OF BATCH CAR, BUILT BY ATLAS CAR & ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    DETAIL VIEW OF BATCH CAR, BUILT BY ATLAS CAR & MANUFACTURING COMPANY. BATCH STORAGE SILOS IN BACKGROUND - Chambers Window Glass Company, Batch Plant, North of Drey (Nineteenth) Street, West of Constitution Boulevard, Arnold, Westmoreland County, PA

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