DARK-FIELD ILLUMINATION SYSTEM
Norgren, D.U.
1962-07-24
A means was developed for viewing objects against a dark background from a viewing point close to the light which illuminates the objects and under conditions where the back scattering of light by the objects is minimal. A broad light retro-directing member on the opposite side of the objects from the light returns direct light back towards the source while directing other light away from the viewing point. The viewing point is offset from the light and thus receives only light which is forwardly scattered by an object while returning towards the source. The object is seen, at its true location, against a dark background. The invention is particularly adapted for illuminating and viewing nuclear particle tracks in a liquid hydrogen bubble chamber through a single chamber window. (AEC)
Veiling glare reduction methods compared for ophthalmic applications
NASA Technical Reports Server (NTRS)
Buchele, D. R.
1981-01-01
Veiling glare in ocular viewing was simulated by viewing the retina of an eye model through a sheet of light-scattering material lit from the front. Four methods of glare reduction were compared, namely, optical scanning, polarized light, viewing and illumination paths either coaxial or intersecting at the object, and closed circuit TV. Photographs show the effect of these methods on visibility. Polarized light was required to eliminate light specularly reflected from the instrument optics. The greatest glare reduction was obtained when the first three methods were utilized together. Glare reduction using TV was limited by nonuniform distribution of scattered light over the image.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Walker, Bennett N., E-mail: bennett.walker@fda.hhs.gov; Office of Device Evaluation, Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, Maryland 20993; James, Robert H.
Glare, glistenings, optical defects, dysphotopsia, and poor image quality are a few of the known deficiencies of intraocular lenses (IOLs). All of these optical phenomena are related to light scatter. However, the specific direction that light scatters makes a critical difference between debilitating glare and a slightly noticeable decrease in image quality. Consequently, quantifying the magnitude and direction of scattered light is essential to appropriately evaluate the safety and efficacy of IOLs. In this study, we introduce a full-angle scanning light scattering profiler (SLSP) as a novel approach capable of quantitatively evaluating the light scattering from IOLs with a nearlymore » 360° view. The SLSP method can simulate in situ conditions by controlling the parameters of the light source including angle of incidence. This testing strategy will provide a more effective nonclinical approach for the evaluation of IOL light scatter.« less
Polar nephelometer for atmospheric particulate studies
NASA Technical Reports Server (NTRS)
Hansen, M. Z.; Evans, W. H.
1980-01-01
A polar nephelometer for use in studying atmospheric aerosols was developed. The nephelometer detects molecular scatter from air and measures scattering from very clean air using pure molecular scattering for calibration. A compact system using a folded light path with an air cooled argon laser for the light source was designed. A small, sensitive detector unit permits easy angular rotation for changing the scattering angle. A narrow detector field of view of + or - 1/4 degree of scattering along with a single wavelength of incident light is used to minimize uncertainties in the scattering theory. The system is automated for data acquisition of the scattering matrix elements over an angular range from 2 degrees to 178 degrees of scattering. Both laser output and detector sensitivity are monitored to normalize the measured light scattering.
Chen, Shuming; Kwok, Hoi Sing
2010-01-04
Light extraction from organic light-emitting diodes (OLEDs) by scattering the light is one of the effective methods for large-area lighting applications. In this paper, we present a very simple and cost-effective method to rough the substrates and hence to scatter the light. By simply sand-blasting the edges and back-side surface of the glass substrates, a 20% improvement of forward efficiency has been demonstrated. Moreover, due to scattering effect, a constant color over all viewing angles and uniform light pattern with Lambertian distribution has been obtained. This simple and cost-effective method may be suitable for mass production of large-area OLEDs for lighting applications.
Methods and apparatus for transparent display using scattering nanoparticles
Hsu, Chia Wei; Qiu, Wenjun; Zhen, Bo; Shapira, Ofer; Soljacic, Marin
2017-06-14
Transparent displays enable many useful applications, including heads-up displays for cars and aircraft as well as displays on eyeglasses and glass windows. Unfortunately, transparent displays made of organic light-emitting diodes are typically expensive and opaque. Heads-up displays often require fixed light sources and have limited viewing angles. And transparent displays that use frequency conversion are typically energy inefficient. Conversely, the present transparent displays operate by scattering visible light from resonant nanoparticles with narrowband scattering cross sections and small absorption cross sections. More specifically, projecting an image onto a transparent screen doped with nanoparticles that selectively scatter light at the image wavelength(s) yields an image on the screen visible to an observer. Because the nanoparticles scatter light at only certain wavelengths, the screen is practically transparent under ambient light. Exemplary transparent scattering displays can be simple, inexpensive, scalable to large sizes, viewable over wide angular ranges, energy efficient, and transparent simultaneously.
Methods and apparatus for transparent display using scattering nanoparticles
Hsu, Chia Wei; Qiu, Wenjun; Zhen, Bo; Shapira, Ofer; Soljacic, Marin
2016-05-10
Transparent displays enable many useful applications, including heads-up displays for cars and aircraft as well as displays on eyeglasses and glass windows. Unfortunately, transparent displays made of organic light-emitting diodes are typically expensive and opaque. Heads-up displays often require fixed light sources and have limited viewing angles. And transparent displays that use frequency conversion are typically energy inefficient. Conversely, the present transparent displays operate by scattering visible light from resonant nanoparticles with narrowband scattering cross sections and small absorption cross sections. More specifically, projecting an image onto a transparent screen doped with nanoparticles that selectively scatter light at the image wavelength(s) yields an image on the screen visible to an observer. Because the nanoparticles scatter light at only certain wavelengths, the screen is practically transparent under ambient light. Exemplary transparent scattering displays can be simple, inexpensive, scalable to large sizes, viewable over wide angular ranges, energy efficient, and transparent simultaneously.
Lee, Ji-Hoon; Lee, Jung Jin; Lim, Young Jin; Kundu, Sudarshan; Kang, Shin-Woong; Lee, Seung Hee
2013-11-04
Long standing electro-optic problems of a polymer-dispersed liquid crystal (PDLC) such as low contrast ratio and transmittances decrease in oblique viewing angle have been challenged with a mixture of dual frequency liquid crystal (DFLC) and reactive mesogen (RM). The DFLC and RM molecules were vertically aligned and then photo-polymerized using a UV light. At scattering state under 50 kHz electric field, DFLC was switched to planar state, giving greater extraordinary refractive index than the normal PDLC cell. Consequently, the scattering intensity and the contrast ratio were increased compared to the conventional PDLC cell. At transparent state under 1 kHz electric field, the extraordinary refractive index of DFLC was simultaneously matched with the refractive index of vertically aligned RM so that the light scattering in oblique viewing angles was minimized, giving rise to high transmittance in all viewing angles.
Flexible detection optics for light scattering
NASA Astrophysics Data System (ADS)
Taratuta, Victor G.; Hurd, Alan J.; Meyer, Robert B.
1984-05-01
We have designed and built a compact, modular apparatus for the collection, viewing, and detection of scattered light for less than 1200, based on a commercially available optical bench. The novelty of our instrument is that it has the flexibility of modular design while allowing the user to see exactly what is happening: both the real image of the sample and the spatial coherence of the scattered light can be examined. There is built-in control over polarization, filtering, magnification, and other parameters.
Mesoscopic coherence in light scattering from cold, optically dense and disordered atomic systems
NASA Astrophysics Data System (ADS)
Kupriyanov, D. V.; Sokolov, I. M.; Havey, M. D.
2017-02-01
Coherent effects manifested in light scattering from cold, optically dense and disordered atomic systems are reviewed from a primarily theoretical point of view. Development of the basic theoretical tools is then elaborated through several physical atomic physics based processes which have been at least partly explored experimentally. These include illustrations drawn from the coherent backscattering effect, random lasing in atomic gases, quantum memories and light-atoms interface assisted by the light trapping mechanism. Current understanding and challenges associated with the transition to high atomic densities and cooperativity in the scattering process are also discussed in some detail.
Light scattering by marine algae: two-layer spherical and nonspherical models
NASA Astrophysics Data System (ADS)
Quirantes, Arturo; Bernard, Stewart
2004-11-01
Light scattering properties of algae-like particles are modeled using the T-matrix for coated scatterers. Two basic geometries have been considered: off-centered coated spheres and centered spheroids. Extinction, scattering and absorption efficiencies, plus scattering in the backward plane, are compared to simpler models like homogeneous (Mie) and coated (Aden-Kerker) models. The anomalous diffraction approximation (ADA), of widespread use in the oceanographic light-scattering community, has also been used as a first approximation, for both homogeneous and coated spheres. T-matrix calculations show that some light scattering values, such as extinction and scattering efficiencies, have little dependence on particle shape, thus reinforcing the view that simpler (Mie, Aden-Kerker) models can be applied to infer refractive index (RI) data from absorption curves. The backscattering efficiency, on the other hand, is quite sensitive to shape. This calls into question the use of light scattering techniques where the phase function plays a pivotal role, and can help explain the observed discrepancy between theoretical and experimental values of the backscattering coefficient in observed in oceanic studies.
Scattered light characterization of FORTIS
NASA Astrophysics Data System (ADS)
McCandliss, Stephan R.; Carter, Anna; Redwine, Keith; Teste, Stephane; Pelton, Russell; Hagopian, John; Kutyrev, Alexander; Li, Mary J.; Moseley, S. Harvey
2017-08-01
We describe our efforts to build a Wide-Field Lyman alpha Geocoronal simulator (WFLaGs) for characterizing the end-to-end sensitivity of FORTIS (Far-UV Off Rowland-circle Telescope for Imaging and Spectroscopy) to scattered Lyman α emission from outside of the nominal (1/2 degree)2 field-of-view. WFLaGs is a 50 mm diameter F/1 aluminum parabolic collimator fed by a hollow cathode discharge lamp with a 80 mm clear MgF2 window housed in a vacuum skin. It creates emission over a 10 degree FOV. WFLaGS will allow us to validate and refine a recently developed scattered light model and verify our scatter light mitigation strategies, which will incorporate low scatter baffle materials, and possibly 3-d printed light traps, covering exposed scatter centers. We present measurements of scattering intensity of Lyman alpha as a function of angle with respect to the specular reflectance direction for several candidate baffle materials. Initial testing of WFLaGs will be described.
Airborne Laser Polar Nephelometer
NASA Technical Reports Server (NTRS)
Grams, Gerald W.
1973-01-01
A polar nephelometer has been developed at NCAR to measure the angular variation of the intensity of light scattered by air molecules and particles. The system has been designed for airborne measurements using outside air ducted through a 5-cm diameter airflow tube; the sample volume is that which is common to the intersection of a collimated source beam and the detector field of view within the airflow tube. The source is a linearly polarized helium-neon laser beam. The optical system defines a collimated field-of-view (0.5deg half-angle) through a series of diaphragms located behind a I72-mm focal length objective lens. A photomultiplier tube is located immediately behind an aperture in the focal plane of the objective lens. The laser beam is mechanically chopped (on-off) at a rate of 5 Hz; a two-channel pulse counter, synchronized to the laser output, measures the photomultiplier pulse rate with the light beam both on and off. The difference in these measured pulse rates is directly proportional to the intensity of the scattered light from the volume common to the intersection of the laser beam and the detector field-of-view. Measurements can be made at scattering angles from 15deg to 165deg with reference to the direction of propagation of the light beam. Intermediate angles are obtained by selecting the angular increments desired between these extreme angles (any multiple of 0.1deg can be selected for the angular increment; 5deg is used in normal operation). Pulses provided by digital circuits control a stepping motor which sequentially rotates the detector by pre-selected angular increments. The synchronous photon-counting system automatically begins measurement of the scattered-light intensity immediately after the rotation to a new angle has been completed. The instrument has been flown on the NASA Convair 990 airborne laboratory to obtain data on the complex index of refraction of atmospheric aerosols. A particle impaction device is operated simultaneously to collect particles from the same airflow tube used to make the scattered-light measurements. A size distribution function is obtained by analysis of the particles collected by the impaction device. Calculated values of the angular variation of the scattered-light intensity are obtained by applying Mie scattering theory to the observed size distribution function and assuming different values of the complex index of refraction of the particles. The calculated values are then compared with data on the actual variation of the scattered-light intensity obtained with the polar nephelometer. The most probable value of the complex refractive index is that which provides the best fit between the experimental light scattering data and the parameters calculated from the observed size distribution function.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Schaefer, Tim; Institut für Physikalische Chemie, Universität zu Köln, 50939 Köln; Schwab, Tobias
A random scattering approach to enhance light extraction in white top-emitting organic light-emitting diodes (OLEDs) is reported. Through solution processing from fluorinated solvents, a nano-particle scattering layer (NPSL) can be deposited directly on top of small molecule OLEDs without affecting their electrical performance. The scattering length for light inside the NPSL is determined from transmission measurements and found to be in agreement with Mie scattering theory. Furthermore, the dependence of the light outcoupling enhancement on electron transport layer thickness is studied. Depending on the electron transport layer thickness, the NPSL enhances the external quantum efficiency of the investigated white OLEDsmore » by between 1.5 and 2.3-fold. For a device structure that has been optimized prior to application of the NPSL, the maximum external quantum efficiency is improved from 4.7% to 7.4% (1.6-fold improvement). In addition, the scattering layer strongly reduces the undesired shift in emission color with viewing angle.« less
NASA Astrophysics Data System (ADS)
van der Laan, John D.; Wright, Jeremy B.; Scrymgeour, David A.; Kemme, Shanalyn A.; Dereniak, Eustace L.
2016-05-01
We present experimental and simulation results for a laboratory-based forward-scattering environment, where 1 μm diameter polystyrene spheres are suspended in water to model the optical scattering properties of fog. Circular polarization maintains its degree of polarization better than linear polarization as the optical thickness of the scattering environment increases. Both simulation and experiment quantify circular polarization's superior persistence, compared to that of linear polarization, and show that it is much less affected by variations in the field of view and collection area of the optical system. Our experimental environment's lateral extent was physically finite, causing a significant difference between measured and simulated degree of polarization values for incident linearly polarized light, but not for circularly polarized light. Through simulation we demonstrate that circular polarization is less susceptible to the finite environmental extent as well as the collection optic's limiting configuration.
NASA Astrophysics Data System (ADS)
Phillips, C. B.; Valenti, M.
2009-12-01
Jupiter's moon Europa likely possesses an ocean of liquid water beneath its icy surface, but estimates of the thickness of the surface ice shell vary from a few kilometers to tens of kilometers. Color images of Europa reveal the existence of a reddish, non-ice component associated with a variety of geological features. The composition and origin of this material is uncertain, as is its relationship to Europa's various landforms. Published analyses of Galileo Near Infrared Mapping Spectrometer (NIMS) observations indicate the presence of highly hydrated sulfate compounds. This non-ice material may also bear biosignatures or other signs of biotic material. Additional spectral information from the Galileo Solid State Imager (SSI) could further elucidate the nature of the surface deposits, particularly when combined with information from the NIMS. However, little effort has been focused on this approach because proper calibration of the color image data is challenging, requiring both skill and patience to process the data and incorporate the appropriate scattered light correction. We are currently working to properly calibrate the color SSI data. The most important and most difficult issue to address in the analysis of multispectral SSI data entails using thorough calibrations and a correction for scattered light. Early in the Galileo mission, studies of the Galileo SSI data for the moon revealed discrepancies of up to 10% in relative reflectance between images containing scattered light and images corrected for scattered light. Scattered light adds a wavelength-dependent low-intensity brightness factor to pixels across an image. For example, a large bright geological feature located just outside the field of view of an image will scatter extra light onto neighboring pixels within the field of view. Scattered light can be seen as a dim halo surrounding an image that includes a bright limb, and can also come from light scattered inside the camera by dirt, edges, and the interfaces of lenses. Because of the wavelength dependence of this effect, a scattered light correction must be performed on any SSI multispectral dataset before quantitative spectral analysis can be done. The process involves using a point-spread function for each filter that helps determine the amount of scattered light expected for a given pixel based on its location and the model attenuation factor for that pixel. To remove scattered light for a particular image taken through a particular filter, the Fourier transform of the attenuation function, which is the point spread function for that filter, is convolved with the Fourier transform of the image at the same wavelength. The result is then filtered for noise in the frequency domain, and then transformed back to the spatial domain. This results in a version of the original image that would have been taken without the scattered light contribution. We will report on our initial results from this calibration.
2016-11-21
Surface features are visible on Saturn's moon Prometheus in this view from NASA's Cassini spacecraft. Most of Cassini's images of Prometheus are too distant to resolve individual craters, making views like this a rare treat. Saturn's narrow F ring, which makes a diagonal line beginning at top center, appears bright and bold in some Cassini views, but not here. Since the sun is nearly behind Cassini in this image, most of the light hitting the F ring is being scattered away from the camera, making it appear dim. Light-scattering behavior like this is typical of rings comprised of small particles, such as the F ring. This view looks toward the unilluminated side of the rings from about 14 degrees below the ring plane. The image was taken in visible light with the Cassini spacecraft narrow-angle camera on Sept. 24, 2016. The view was acquired at a distance of approximately 226,000 miles (364,000 kilometers) from Prometheus and at a sun-Prometheus-spacecraft, or phase, angle of 51 degrees. Image scale is 1.2 miles (2 kilometers) per pixel. http://photojournal.jpl.nasa.gov/catalog/PIA20508
How Noniridescent Colors Are Generated by Quasi-ordered Structures of Bird Feathers
DOE Office of Scientific and Technical Information (OSTI.GOV)
Noh, Heeso; Liew, Seng Fatt; Saranathan, Vinodkumar
2012-03-26
We investigate the mechanism of structural coloration by quasi-ordered nanostructures in bird feather barbs. Small-angle X-ray scattering (SAXS) data reveal the structures are isotropic and have short-range order on length scales comparable to optical wavelengths. We perform angle-resolved reflection and scattering spectrometry to fully characterize the colors under directional and omni-directional illumination of white light. Under directional lighting, the colors change with the angle between the directions of illumination and observation. The angular dispersion of the primary peaks in the scattering/reflection spectra can be well explained by constructive interference of light that is scattered only once in the quasi-ordered structures.more » Using the Fourier power spectra of structure from the SAXS data we calculate optical scattering spectra and explain why the light scattering peak is the highest in the backscattering direction. Under omni-directional lighting, colors from the quasi-ordered structures are invariant with the viewing angle. The non-iridescent coloration results from the isotropic nature of structures instead of strong backscattering.« less
Measurement and Modeling of the Optical Scattering Properties of Crop Canopies
NASA Technical Reports Server (NTRS)
Vanderbilt, V. C.; Grant, L.
1984-01-01
Efforts in measuring, analyzing, and mathematically modeling the specular, polarized, and diffuse light scattering properties of several plant canopies and their component parts (leaves, stems, fruit, soil) as a function of view angle and illumination angle are reported. Specific objectives were: (1) to demonstrate a technique for determining the specular and diffuse components of the reflectance factor of plant canopies; (2) to acquire the measurements and begin assembling a data set for developing and testing canopy reflectance models; (3) to design and build a new optical instrument to measure the light scattering properties of individual leaves; and (4) to use this instrument to survey and investigate the information in the light scattering properties of individual leaves of crops, forests, weeds, and horticulture.
Wide-field high spatial frequency domain imaging of tissue microstructure
NASA Astrophysics Data System (ADS)
Lin, Weihao; Zeng, Bixin; Cao, Zili; Zhu, Danfeng; Xu, M.
2018-02-01
Wide-field tissue imaging is usually not capable of resolving tissue microstructure. We present High Spatial Frequency Domain Imaging (HSFDI) - a noncontact imaging modality that spatially maps the tissue microscopic scattering structures over a large field of view. Based on an analytical reflectance model of sub-diffusive light from forward-peaked highly scattering media, HSFDI quantifies the spatially-resolved parameters of the light scattering phase function from the reflectance of structured light modulated at high spatial frequencies. We have demonstrated with ex vivo cancerous tissue to validate the robustness of HSFDI in significant contrast and differentiation of the microstructutral parameters between different types and disease states of tissue.
Underwater Docking of Autonomous Undersea Vehicles Using Optical Terminal Guidance
1997-10-01
simply using a brighter beacon. Scattered light within the field-of-view is imaged almost equally into each of the four quadrants of a photodetector...received by adjacent quadrants a great deal of the forward scattered light can be rejected when the signals from the four quadrants are subsequently...employed to aim the nose of the vehicle at the light source because the illumination is equal in all four quadrants only when the tracker’s optical axis is
Characterization of the angular memory effect of scattered light in biological tissues.
Schott, Sam; Bertolotti, Jacopo; Léger, Jean-Francois; Bourdieu, Laurent; Gigan, Sylvain
2015-05-18
High resolution optical microscopy is essential in neuroscience but suffers from scattering in biological tissues and therefore grants access to superficial brain layers only. Recently developed techniques use scattered photons for imaging by exploiting angular correlations in transmitted light and could potentially increase imaging depths. But those correlations ('angular memory effect') are of a very short range and should theoretically be only present behind and not inside scattering media. From measurements on neural tissues and complementary simulations, we find that strong forward scattering in biological tissues can enhance the memory effect range and thus the possible field-of-view by more than an order of magnitude compared to isotropic scattering for ∼1 mm thick tissue layers.
Aqueous carrier waveguide in a flow cytometer
Mariella, Jr., Raymond P.; van den Engh, Gerrit; Northrup, M. Allen
1995-01-01
The liquid of a flow cytometer itself acts as an optical waveguide, thus transmitting the light to an optical filter/detector combination. This alternative apparatus and method for detecting scattered light in a flow cytometer is provided by a device which views and detects the light trapped within the optical waveguide formed by the flow stream. A fiber optic or other light collecting device is positioned within the flow stream. This provides enormous advantages over the standard light collection technique which uses a microscope objective. The signal-to-noise ratio is greatly increased over that for right-angle-scattered light collected by a microscope objective, and the alignment requirements are simplified.
LED lamp or bulb with remote phosphor and diffuser configuration with enhanced scattering properties
Tong, Tao; Le Toquin, Ronan; Keller, Bernd; Tarsa, Eric; Youmans, Mark; Lowes, Theodore; Medendorp, Jr., Nicholas W; Van De Ven, Antony; Negley, Gerald
2014-11-11
An LED lamp or bulb is disclosed that comprises a light source, a heat sink structure and an optical cavity. The optical cavity comprises a phosphor carrier having a conversions material and arranged over an opening to the cavity. The phosphor carrier comprises a thermally conductive transparent material and is thermally coupled to the heat sink structure. An LED based light source is mounted in the optical cavity remote to the phosphor carrier with light from the light source passing through the phosphor carrier. A diffuser dome is included that is mounted over the optical cavity, with light from the optical cavity passing through the diffuser dome. The properties of the diffuser, such as geometry, scattering properties of the scattering layer, surface roughness or smoothness, and spatial distribution of the scattering layer properties may be used to control various lamp properties such as color uniformity and light intensity distribution as a function of viewing angle.
2006-08-23
With Saturn terminator as a backdrop, this view of the unlit face of the rings makes it easy to distinguish between areas that are actual gaps, where light passes through essentially unimpeded, and areas where the rings block or scatter light
High-throughput isotropic mapping of whole mouse brain using multi-view light-sheet microscopy
NASA Astrophysics Data System (ADS)
Nie, Jun; Li, Yusha; Zhao, Fang; Ping, Junyu; Liu, Sa; Yu, Tingting; Zhu, Dan; Fei, Peng
2018-02-01
Light-sheet fluorescence microscopy (LSFM) uses an additional laser-sheet to illuminate selective planes of the sample, thereby enabling three-dimensional imaging at high spatial-temporal resolution. These advantages make LSFM a promising tool for high-quality brain visualization. However, even by the use of LSFM, the spatial resolution remains insufficient to resolve the neural structures across a mesoscale whole mouse brain in three dimensions. At the same time, the thick-tissue scattering prevents a clear observation from the deep of brain. Here we use multi-view LSFM strategy to solve this challenge, surpassing the resolution limit of standard light-sheet microscope under a large field-of-view (FOV). As demonstrated by the imaging of optically-cleared mouse brain labelled with thy1-GFP, we achieve a brain-wide, isotropic cellular resolution of 3μm. Besides the resolution enhancement, multi-view braining imaging can also recover complete signals from deep tissue scattering and attenuation. The identification of long distance neural projections across encephalic regions can be identified and annotated as a result.
Waveguide detection of right-angle-scattered light in flow cytometry
Mariella, Jr., Raymond P.
2000-01-01
A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.
Aqueous carrier waveguide in a flow cytometer
Mariella, R.P. Jr.; Engh, G. van den; Northrup, M.A.
1995-12-12
The liquid of a flow cytometer itself acts as an optical waveguide, thus transmitting the light to an optical filter/detector combination. This alternative apparatus and method for detecting scattered light in a flow cytometer is provided by a device which views and detects the light trapped within the optical waveguide formed by the flow stream. A fiber optic or other light collecting device is positioned within the flow stream. This provides enormous advantages over the standard light collection technique which uses a microscope objective. The signal-to-noise ratio is greatly increased over that for right-angle-scattered light collected by a microscope objective, and the alignment requirements are simplified. 6 figs.
Frequency-scanning particle size spectrometer
NASA Technical Reports Server (NTRS)
Fymat, A. L. (Inventor)
1979-01-01
A particle size spectrometer having a fixed field of view within the forward light scattering cone at an angle theta sub s between approximately 100 and 200 minutes of arc (preferably at 150 minutes), a spectral range extending approximately from 0.2 to 4.0 inverse micrometers, and a spectral resolution between about 0.1 and 0.2 inverse micrometers (preferably toward the lower end of this range of spectral resolution), is employed to determine the distribution of particle sizes, independently of the chemical composition of the particles, from measurements of incident light, at each frequency, sigma (=1/lambda), and scattered light, I(sigma).
NASA Astrophysics Data System (ADS)
Kocifaj, Miroslav
2018-02-01
The mechanism in which multiple scattering influences the radiance of a night sky has been poorly quantified until recently, or even completely unknown from the theoretical point of view. In this paper, the relative contribution of higher-scattering radiances to the total sky radiance is treated analytically for all orders of scattering, showing that a fast and accurate numerical solution to the problem exists. Unlike a class of ray tracing codes in which CPU requirements increase tremendously with each new scattering mode, the solution developed here requires the same processor time for each scattering mode. This allows for rapid estimation of higher-scattering radiances and residual error that is otherwise unknown if these radiances remain undetermined. Such convergence testing is necessary to guarantee accuracy and the stability of the numerical predictions. The performance of the method developed here is demonstrated in a set of numerical experiments aiming to uncover the relative importance of higher-scattering radiances at different distances from a light source. We have shown, that multiple scattering effects are generally low if distance to the light source is below 30 km. At large distances the multiple scattering can become important at the dark sky elements situated opposite to the light source. However, the brightness at this part of sky is several orders of magnitude smaller than that of a glowing dome of light over a city, so we do not expect that a partial increase or even doubling the radiance of otherwise dark sky elements can noticeably affect astronomical observations or living organisms (including humans). Single scattering is an appropriate approximation to the sky radiance of a night sky in the vast majority of cases.
Wide-field imaging through scattering media by scattered light fluorescence microscopy
NASA Astrophysics Data System (ADS)
Zhou, Yulan; Li, Xun
2017-08-01
To obtain images through scattering media, scattered light fluorescence (SLF) microscopy that utilizes the optical memory effect has been developed. However, the small field of view (FOV) of SLF microscopy limits its application. In this paper, we have introduced a re-modulation method to achieve wide-field imaging through scattering media by SLF microscopy. In the re-modulation method, to raster scan the focus across the object plane, the incident wavefront is re-modulated via a spatial light modulator (SLM) in the updated phase compensation calculated using the optimized iterative algorithm. Compared with the conventional optical memory effect method, the re-modulation method can greatly increase the FOV of a SLF microscope. With the phase compensation theoretically calculated, the process of updating the phase compensation of a high speed SLM is fast. The re-modulation method does not increase the imaging time. The re-modulation method is, therefore, expected to make SLF microscopy have much wider applications in biology, medicine and physiology.
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...
NASA Astrophysics Data System (ADS)
Gritsevich, M.; Penttilä, A.; Maconi, G.; Kassamakov, I.; Martikainen, J.; Markkanen, J.; Vaisanen, T.; Helander, P.; Puranen, T.; Salmi, A.; Hæggström, E.; Muinonen, K.
2017-12-01
Electromagnetic scattering is a fundamental physical process that allows inferring characteristics of an object studied remotely. This possibility is enhanced by obtaining the light-scattering response at multiple wavelengths and viewing geometries, i.e., by considering a wider range of the phase angle (the angle between the incident light and the light reflected from the object) in the experiment. Within the ERC Advanced Grant project SAEMPL (http://cordis.europa.eu/project/rcn/107666_en.html) we have assembled an interdisciplinary group of scientists to develop a fully automated, 3D scatterometer that can measure scattered light at different wavelengths from small particulate samples. The setup comprises: (a) the PXI Express platform to synchronously record data from several photomultiplier tubes (PMTs); (b) a motorized rotation stage to precisely control the azimuthal angle of the PMTs around 360°; and (c) a versatile light source, whose wavelength, polarization, intensity, and beam shape can be precisely controlled. An acoustic levitator is used to hold the sample without touching it. The device is the first of its kind, since it measures controlled spectral angular scattering including all polarization effects, for an arbitrary object in the µm-cm size scale. It permits a nondestructive, disturbance-free measurement with control of the orientation and location of the scattering object. To demonstrate our approach we performed detailed measurements of light scattered by a Chelyabinsk LL5 chondrite particle, derived from the light-colored lithology sample of the meteorite. These measurements are cross-validated against the modeled light-scattering characteristics of the sample, i.e., the intensity and the degree of linear polarization of the reflected light, calculated with state-of-the-art electromagnetic techniques (see Muinonen et al., this meeting). We demonstrate a unique non-destructive approach to derive the optical properties of small grain samples which facilitates research on highly valuable planetary materials, such as samples returned from space missions or rare meteorites.
Prediction of Viking lander camera image quality
NASA Technical Reports Server (NTRS)
Huck, F. O.; Burcher, E. E.; Jobson, D. J.; Wall, S. D.
1976-01-01
Formulations are presented that permit prediction of image quality as a function of camera performance, surface radiance properties, and lighting and viewing geometry. Predictions made for a wide range of surface radiance properties reveal that image quality depends strongly on proper camera dynamic range command and on favorable lighting and viewing geometry. Proper camera dynamic range commands depend mostly on the surface albedo that will be encountered. Favorable lighting and viewing geometries depend mostly on lander orientation with respect to the diurnal sun path over the landing site, and tend to be independent of surface albedo and illumination scattering function. Side lighting with low sun elevation angles (10 to 30 deg) is generally favorable for imaging spatial details and slopes, whereas high sun elevation angles are favorable for measuring spectral reflectances.
Multi-Component, Multi-Point Interferometric Rayleigh/Mie Doppler Velocimeter
NASA Technical Reports Server (NTRS)
Danehy, Paul M.; Lee, Joseph W.; Bivolaru, Daniel
2012-01-01
An interferometric Rayleigh scattering system was developed to enable the measurement of multiple, orthogonal velocity components at several points within very-high-speed or high-temperature flows. The velocity of a gaseous flow can be optically measured by sending laser light into the gas flow, and then measuring the scattered light signal that is returned from matter within the flow. Scattering can arise from either gas molecules within the flow itself, known as Rayleigh scattering, or from particles within the flow, known as Mie scattering. Measuring Mie scattering is the basis of all commercial laser Doppler and particle imaging velocimetry systems, but particle seeding is problematic when measuring high-speed and high-temperature flows. The velocimeter is designed to measure the Doppler shift from only Rayleigh scattering, and does not require, but can also measure, particles within the flow. The system combines a direct-view, large-optic interferometric setup that calculates the Doppler shift from fringe patterns collected with a digital camera, and a subsystem to capture and re-circulate scattered light to maximize signal density. By measuring two orthogonal components of the velocity at multiple positions in the flow volume, the accuracy and usefulness of the flow measurement increase significantly over single or nonorthogonal component approaches.
Polymer Dispersed Liquid Crystal Displays
NASA Astrophysics Data System (ADS)
Doane, J. William
The following sections are included: * INTRODUCTION AND HISTORICAL DEVELOPMENT * PDLC MATERIALS PREPARATION * Polymerization induced phase separation (PIPS) * Thermally induced phase separation (TIPS) * Solvent induced phase separation (SIPS) * Encapsulation (NCAP) * RESPONSE VOLTAGE * Dielectric and resistive effects * Radial configuration * Bipolar configuration * Other director configurations * RESPONSE TIME * DISPLAY CONTRAST * Light scattering and index matching * Incorporation of dyes * Contrast measurements * PDLC DISPLAY DEVICES AND INNOVATIONS * Reflective direct view displays * Large-scale, flexible displays * Switchable windows * Projection displays * High definition spatial light modulator * Haze-free PDLC shutters: wide angle view displays * ENVIRONMENTAL STABILITY * ACKNOWLEDGEMENTS * REFERENCES
Federal Register 2010, 2011, 2012, 2013, 2014
2011-03-15
... CONTACT section to view the hard copy of the docket. You may view the hard copy of the docket Monday... Submittals Today's proposed action concerns two submittals from California. The first submittal from the... impairs visibility by scattering and absorbing light. Visibility impairment reduces the clarity, color...
DOE Office of Scientific and Technical Information (OSTI.GOV)
Kihara, Toshiki
2007-09-01
A phase unwrapping method that employs scattered-light photoelasticity with unpolarized light was proposed for automated three-dimensional stress analysis [Appl. Opt. 45, 8848 (2006)]. I now demonstrate the validity of this method by performing nondestructive measurements at three different wavelengths of the secondary principal stress direction {psi}j and the total relative phase retardation {rho}jtot in the plane that contains the rotated principal stress directions in a spherical frozen stress model and compare the results obtained with mechanically sliced models. The parameters {psi}j and {rho}jtot were measured nondestructively over the entire field of view for the first time, to the best ofmore » my knowledge.« less
Physical behaviour of anthropogenic light propagation into the nocturnal environment
Aubé, Martin
2015-01-01
Propagation of artificial light at night (ALAN) in the environment is now known to have non negligible consequences on fauna, flora and human health. These consequences depend on light levels and their spectral power distributions, which in turn rely on the efficiency of various physical processes involved in the radiative transfer of this light into the atmosphere and its interactions with the built and natural environment. ALAN can affect the living organisms by direct lighting and indirect lighting (scattered by the sky and clouds and/or reflected by local surfaces). This paper mainly focuses on the behaviour of the indirect light scattered under clear sky conditions. Various interaction processes between anthropogenic light sources and the natural environment are discussed. This work mostly relies on a sensitivity analysis conducted with the light pollution radiative transfer model, Illumina (Aubé et al. 2005 Light pollution modelling and detection in a heterogeneous environment: toward a night-time aerosol optical depth retrieval method. In Proc. SPIE 2005, vol. 5890, San Diego, California, USA). More specifically, the impact of (i) the molecular and aerosol scattering and absorption, (ii) the second order of scattering, (iii) the topography and obstacle blocking, (iv) the ground reflectance and (v) the spectrum of light devices and their angular emission functions are examined. This analysis considers different behaviour as a function of the distance from the city centre, along with different zenith viewing angles in the principal plane. PMID:25780231
Physical behaviour of anthropogenic light propagation into the nocturnal environment.
Aubé, Martin
2015-05-05
Propagation of artificial light at night (ALAN) in the environment is now known to have non negligible consequences on fauna, flora and human health. These consequences depend on light levels and their spectral power distributions, which in turn rely on the efficiency of various physical processes involved in the radiative transfer of this light into the atmosphere and its interactions with the built and natural environment. ALAN can affect the living organisms by direct lighting and indirect lighting (scattered by the sky and clouds and/or reflected by local surfaces). This paper mainly focuses on the behaviour of the indirect light scattered under clear sky conditions. Various interaction processes between anthropogenic light sources and the natural environment are discussed. This work mostly relies on a sensitivity analysis conducted with the light pollution radiative transfer model, Illumina (Aubé et al. 2005 Light pollution modelling and detection in a heterogeneous environment: toward a night-time aerosol optical depth retrieval method. In Proc. SPIE 2005, vol. 5890, San Diego, California, USA). More specifically, the impact of (i) the molecular and aerosol scattering and absorption, (ii) the second order of scattering, (iii) the topography and obstacle blocking, (iv) the ground reflectance and (v) the spectrum of light devices and their angular emission functions are examined. This analysis considers different behaviour as a function of the distance from the city centre, along with different zenith viewing angles in the principal plane. © 2015 The Author(s) Published by the Royal Society. All rights reserved.
First results from the Thomson scattering diagnostic on Proto-MPEX
DOE Office of Scientific and Technical Information (OSTI.GOV)
Biewer, Theodore M; Meitner, Steven J; Rapp, Juergen
2016-01-01
A Thomson scattering diagnostic has been successfully implemented on the prototype Material Plasma Exposure eXperiment (Proto-MPEX) at Oak Ridge National Laboratory. The diagnostic collects the light scattered by plasma electrons and spectroscopically resolves the Doppler shift imparted to the light by the velocity of the electrons. The spread in velocities is proportional to the electron temperature, while the total number of photons is proportional to the electron density. Thomson scattering is a technique used on many devices to measure the electron temperature (Te) and electron density (ne) of the plasma. A challenging aspect of the technique is to discriminate themore » small number of Thomson scattered photons against the large peak of background photons from the high-power laser used to probe the plasma. A variety of methods are used to mitigate the background photons in Proto-MPEX, including Brewster angled windows, viewing dumps, and light baffles. With these methods, first results were measured from Argon plasmas in Proto-MPEX, indicating Te ~ 2 eV and ne ~ 1x1019 m-3. The configuration of the Proto-MPEX Thomson scattering diagnostic will be described and plans for improvement will be given.« less
Biomass in the upwelling areas along the northwest coast of Africa as viewed with ERTS-1
NASA Technical Reports Server (NTRS)
Szekielda, K.; Curran, R. J.
1973-01-01
Light penetration in water is affected by plankton, algae, and dissolved and suspended matter. As a consequence, the composition of backscattered light from below the air-sea interface is determined by the nature of the constituents in the water column. In contrast to the absorption spectrum of chemically pure chlorophyll in solution, algae suspensions absorb and scatter light more uniformly throughout the visible part of the electromagnetic spectrum. Because of their spectral absorption and scattering properties plankton concentration can be estimated by measuring the spectral backscattered radiance over water. Experiments using this approach were performed in upwelling regions along the northwest coast of Africa.
Patterns and properties of polarized light in air and water
Cronin, Thomas W.; Marshall, Justin
2011-01-01
Natural sources of light are at best weakly polarized, but polarization of light is common in natural scenes in the atmosphere, on the surface of the Earth, and underwater. We review the current state of knowledge concerning how polarization and polarization patterns are formed in nature, emphasizing linearly polarized light. Scattering of sunlight or moonlight in the sky often forms a strongly polarized, stable and predictable pattern used by many animals for orientation and navigation throughout the day, at twilight, and on moonlit nights. By contrast, polarization of light in water, while visible in most directions of view, is generally much weaker. In air, the surfaces of natural objects often reflect partially polarized light, but such reflections are rarer underwater, and multiple-path scattering degrades such polarization within metres. Because polarization in both air and water is produced by scattering, visibility through such media can be enhanced using straightforward polarization-based methods of image recovery, and some living visual systems may use similar methods to improve vision in haze or underwater. Although circularly polarized light is rare in nature, it is produced by the surfaces of some animals, where it may be used in specialized systems of communication. PMID:21282165
Design considerations for a backlight with switchable viewing angles
NASA Astrophysics Data System (ADS)
Fujieda, Ichiro; Takagi, Yoshihiko; Rahadian, Fanny
2006-08-01
Small-sized liquid crystal displays are widely used for mobile applications such as cell phones. Electronic control of a viewing angle range is desired in order to maintain privacy for viewing in public as well as to provide wide viewing angles for solitary viewing. Conventionally, a polymer-dispersed liquid crystal (PDLC) panel is inserted between a backlight and a liquid crystal panel. The PDLC layer either transmits or scatters the light from the backlight, thus providing an electronic control of viewing angles. However, such a display system is obviously thick and expensive. Here, we propose to place an electronically-controlled, light-deflecting device between an LED and a light-guide of a backlight. For example, a liquid crystal lens is investigated for other applications and its focal length is controlled electronically. A liquid crystal phase grating either transmits or diffracts an incoming light depending on whether or not a periodic phase distribution is formed inside its liquid crystal layer. A bias applied to such a device will control the angular distribution of the light propagating inside a light-guide. Output couplers built in the light-guide extract the propagating light to outside. They can be V-shaped grooves, pyramids, or any other structures that can refract, reflect or diffract light. When any of such interactions occur, the output couplers translate the changes in the propagation angles into the angular distribution of the output light. Hence the viewing-angle characteristic can be switched. The designs of the output couplers and the LC devices are important for such a backlight system.
Sub-pixel accuracy thickness calculation of poultry fillets from scattered laser profiles
NASA Astrophysics Data System (ADS)
Jing, Hansong; Chen, Xin; Tao, Yang; Zhu, Bin; Jin, Fenghua
2005-11-01
A laser range imaging system based on the triangulation method was designed and implemented for online high-resolution thickness calculation of poultry fillets. A laser pattern was projected onto the surface of the chicken fillet for calculation of the thickness of the meat. Because chicken fillets are relatively loosely-structured material, a laser light easily penetrates the meat, and scattering occurs both at and under the surface. When laser light is scattered under the surface it is reflected back and further blurs the laser line sharpness. To accurately calculate the thickness of the object, the light transportation has to be considered. In the system, the Bidirectional Reflectance Distribution Function (BSSRDF) was used to model the light transportation and the light pattern reflected into the cameras. BSSRDF gives the reflectance of a target as a function of illumination geometry and viewing geometry. Based on this function, an empirical method has been developed and it has been proven that this method can be used to accurately calculate the thickness of the object from a scattered laser profile. The laser range system is designed as a sub-system that complements the X-ray bone inspection system for non-invasive detection of hazardous materials in boneless poultry meat with irregular thickness.
NASA Technical Reports Server (NTRS)
Leblanc, S.; Redemann, Jens; Shinozuka, Yohei; Flynn, Connor J.; Segal Rozenhaimer, Michal; Kacenelenbogen, Meloe Shenandoah; Pistone, Kristina Marie Myers; Schmidt, Sebastian; Cochrane, Sabrina
2016-01-01
We present a first view of data collected during a recent field campaign aimed at measuring biomass burning aerosol above clouds from airborne platforms. The NASA ObseRvations of CLouds above Aerosols and their intEractionS (ORACLES) field campaign recently concluded its first deployment sampling clouds and overlying aerosol layer from the airborne platform NASA P3. We present results from the Spectrometer for Sky-Scanning, Sun-Tracking Atmospheric Research (4STAR), in conjunction with the Solar Spectral Flux Radiometers (SSFR). During this deployment, 4STAR sampled transmitted solar light either via direct solar beam measurements and scattered light measurements, enabling the measurement of aerosol optical thickness and the retrieval of information on aerosol particles in addition to overlying cloud properties. We focus on the zenith-viewing scattered light measurements, which are used to retrieve cloud optical thickness, effective radius, and thermodynamic phase of clouds under a biomass burning layer. The biomass burning aerosol layer present above the clouds is the cause of potential bias in retrieved cloud optical depth and effective radius from satellites. We contrast the typical reflection based approach used by satellites to the transmission based approach used by 4STAR during ORACLES for retrieving cloud properties. It is suspected that these differing approaches will yield a change in retrieved properties since light transmitted through clouds is sensitive to a different cloud volume than reflected light at cloud top. We offer a preliminary view of the implications of these differences in sampling volumes to the calculation of cloud radiative effects (CRE).
NASA Astrophysics Data System (ADS)
Greeley, A.; Neumann, T.; Markus, T.; Kurtz, N. T.; Cook, W. B.
2015-12-01
Existing visible light laser altimeters such as MABEL (Multiple Altimeter Beam Experimental Lidar) - a single photon counting simulator for ATLAS (Advanced Topographic Laser Altimeter System) on NASA's upcoming ICESat-2 mission - and ATM (Airborne Topographic Mapper) on NASA's Operation IceBridge mission provide scientists a view of Earth's ice sheets, glaciers, and sea ice with unprecedented detail. Precise calibration of these instruments is needed to understand rapidly changing parameters like sea ice freeboard and to measure optical properties of surfaces like snow covered ice sheets using subsurface scattered photons. Photons travelling into snow, ice, or water before scattering back to the altimeter receiving system (subsurface photons) travel farther and longer than photons scattering off the surface only, causing a bias in the measured elevation. We seek to identify subsurface photons in a laboratory setting using a flight-tested laser altimeter (MABEL) and to quantify their effect on surface elevation estimates for laser altimeter systems. We also compare these estimates with previous laboratory measurements of green laser light transmission through snow, as well as Monte Carlo simulations of backscattered photons from snow.
NASA Technical Reports Server (NTRS)
Brown, John C.; Fox, Geoffrey K.
1989-01-01
The depolarizing and occultation effects of a finite spherical light source on the polarization of light Thomson-scattered from a flat circumstellar envelope seen edge-on are analyzed. The analysis shows that neglect of the finite size of the light source leads to a gross overestimate of the polarization for a given disk geometry. By including occultation and depolarization, it is found that B-star envelopes are necessarily highly flattened disk-type structures. For a disk viewed edge-on, the effect of occultation reduces the polarization more than the inclusion of the depolarization factor alone. Analysis of a one-dimensional plume leads to a powerful technique that permits the electron density distribution to be explicitly obtained from the polarimetric data.
Nonclassical-light generation in a photonic-band-gap nonlinear planar waveguide
NASA Astrophysics Data System (ADS)
Peřina, Jan, Jr.; Sibilia, Concita; Tricca, Daniela; Bertolotti, Mario
2004-10-01
The optical parametric process occurring in a photonic-band-gap planar waveguide is studied from the point of view of nonclassical-light generation. The nonlinearly interacting optical fields are described by the generalized superposition of coherent signals and noise using the method of operator linear corrections to a classical strong solution. Scattered backward-propagating fields are taken into account. Squeezed light as well as light with sub-Poissonian statistics can be obtained in two-mode fields under the specified conditions.
Methods of satellite oceanography
NASA Technical Reports Server (NTRS)
Stewart, R. H.
1985-01-01
The theoretical basis for remote sensing measurements of climate and ocean dynamics is examined. Consideration is given to: the absorption of electromagnetic radiation in the atmosphere; scattering in the atmosphere; and satellite observations using visible light. Consideration is also given to: the theory of radio scatter from the sea; scatter of centimeter waves from the sea; and the theory of operation of synthetic aperture radars. Additional topics include: the coordinate systems of satellite orbits for oceanographic remote sensing applications; the operating features of the major U.S. satellite systems for viewing the ocean; and satellite altimetry.
Rayleigh-Brillouin light scattering spectroscopy of nitrous oxide (N2O)
NASA Astrophysics Data System (ADS)
Wang, Y.; Liang, K.; van de Water, W.; Marques, W.; Ubachs, W.
2018-02-01
High signal-to-noise and high-resolution light scattering spectra are measured for nitrous oxide (N2O) gas at an incident wavelength of 403.00 nm, at 90° scattering, at room temperature and at gas pressures in the range 0.5 - 4 bar. The resulting Rayleigh-Brillouin light scattering spectra are compared to a number of models describing in an approximate manner the collisional dynamics and energy transfer in this gaseous medium of this polyatomic molecular species. The Tenti-S6 model, based on macroscopic gas transport coefficients, reproduces the scattering profiles in the entire pressure range at less than 2% deviation at a similar level as does the alternative kinetic Grad's 6-moment model, which is based on the internal collisional relaxation as a decisive parameter. A hydrodynamic model fails to reproduce experimental spectra for the low pressures of 0.5-1 bar, but yields very good agreement ( < 1%) in the pressure range 2 - 4 bar. While these three models have a different physical basis the internal molecular relaxation derived can for all three be described in terms of a bulk viscosity of ηb ∼(6 ± 2) ×10-5 Pa · s. A 'rough-sphere' model, previously shown to be effective to describe light scattering in SF6 gas, is not found to be suitable, likely in view of the non-sphericity and asymmetry of the N-N-O structured linear polyatomic molecule.
Large area, label-free imaging of extracellular matrix using telecentricity
NASA Astrophysics Data System (ADS)
Visbal Onufrak, Michelle A.; Konger, Raymond L.; Kim, Young L.
2017-02-01
Subtle alterations in stromal tissue structures and organizations within the extracellular matrix (ECM) have been observed in several types of tissue abnormalities, including early skin cancer and wounds. Current microscopic imaging methods often lack the ability to accurately determine the extent of malignancy over a large area, due to their limited field of view. In this research we focus on the development of simple mesoscopic (i.e. between microscopic and macroscopic) biomedical imaging device for non-invasive assessment of ECM alterations over a large, heterogeneous area. In our technology development, a telecentric lens, commonly used in machine vision systems but rarely used in biomedical imaging, serves as a key platform to visualize alterations in tissue microenvironments in a label-free manner over a clinically relevant area. In general, telecentric imaging represents a simple, alternative method for reducing unwanted scattering or diffuse light caused by the highly anisotropic scattering properties of biological tissue. In particular, under telecentric imaging the light intensity backscattered from biological tissue is mainly sensitive to the scattering anisotropy factor, possibly associated with the ECM. We demonstrate the inherent advantages of combining telecentric lens systems with hyperspectral imaging for providing optical information of tissue scattering in biological tissue of murine models, as well as light absorption of hemoglobin in blood vessel tissue phantoms. Thus, we envision that telecentric imaging could potentially serve for simple site-specific, tissue-based assessment of stromal alterations over a clinically relevant field of view in a label-free manner, for studying diseases associated with disruption of homeostasis in ECM.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Choquet, Élodie; Perrin, Marshall D.; Chen, Christine H.
We present the first images of four debris disks observed in scattered light around the young (4–250 Myr old) M dwarfs TWA 7 and TWA 25, the K6 star HD 35650, and the G2 star HD 377. We obtained these images by reprocessing archival Hubble Space Telescope NICMOS coronagraph data with modern post-processing techniques as part of the Archival Legacy Investigation of Circumstellar Environments program. All four disks appear faint and compact compared with other debris disks resolved in scattered light. The disks around TWA 25, HD 35650, and HD 377 appear very inclined, while TWA 7's disk is viewed nearly face-on. The surface brightness of HD 35650's diskmore » is strongly asymmetric. These new detections raise the number of disks resolved in scattered light around M and late-K stars from one (the AU Mic system) to four. This new sample of resolved disks enables comparative studies of heretofore scarce debris disks around low-mass stars relative to solar-type stars.« less
Astronomy in Denver: Polarization of bow shock nebulae around massive stars
NASA Astrophysics Data System (ADS)
Shrestha, Manisha; Hoffman, Jennifer L.; Ignace, Richard; Neilson, Hilding; Richard Ignace
2018-06-01
Stellar wind bow shocks are structures created when stellar winds with supersonic relative velocities interact with the local interstellar medium (ISM). They can be studied to understand the properties of stars as well as the ISM. Since bow shocks are asymmetric, light becomes polarized by scattering in the regions of enhanced density they create. We use a Monte Carlo radiative transfer code calle SLIP to simulate the polarization signatures produced by both resolved and unresolved bow shocks with analytically derived shapes and density structures. When electron scattering is the polarizing mechanism, we find that optical depth plays an important role in the polarization signatures. While results for low optical depths reproduce theoretical predictions, higher optical depths produce higher polarization and position angle rotations at specific viewing angles. This is due to the geometrical properties of the bow shock along with multiple scattering effects. For dust scattering, we find that the polarization signature is strongly affected by wavelength, dust size, dust composition, and viewing angle. Depending on the viewing angle, the polarization magnitude may increase or decrease as a function of wavelength. We will present results from these simulations and preliminary comparisons with observational data.
STS-67 sunset and earth limb view
1995-03-17
STS067-709-007 (2-18 March 1995) --- This shot, taken just after sunset, shows several distinct layers of gases in the atmosphere on the Earths limb. The distinct colors formed by the amount of scattered light that passes through these gases.
Atmospheric Science Data Center
2013-04-17
article title: Hudson Bay and James Bay, Canada ... which scatters more light in the backward direction. This example illustrates how multi-angle viewing can distinguish physical structures ... MD. The MISR data were obtained from the NASA Langley Research Center Atmospheric Science Data Center in Hampton, VA. Image ...
1971-08-01
S71-58222 (31 July-2 Aug. 1971) --- During the lunar eclipse that occurred during the Apollo 15 lunar landing mission, astronaut Alfred M. Worden, command module pilot, used a 35mm Nikon camera to obtain a series of 15 photographs while the moon was entering and exiting Earth's umbra. Although it might seem that there should be no light on the moon when it is in Earth's shadow, sunlight is scattered into this region by Earth's atmosphere. This task was an attempt to measure by photographic photometry the amount of scattered light reaching the moon. The four views from upper left to lower right were selected to show the moon as it entered Earth's umbra. The first is a four-second exposure which was taken at the moment when the moon had just entered umbra; the second is a 15-second exposure taken two minutes after entry; the third, a 30-second exposure three minutes after entry; and the fourth is a 60-second exposure four minutes after entry. In all cases the light reaching the moon was so bright on the very high speed film (Eastman Kodak type 2485 emulsion) that the halation obscures the lunar image, which should be about one-third as big as the circle of light. The background star field is clearly evident, and this is very important for these studies. The spacecraft was in full sunlight when these photographs were taken, and it was pointed almost directly away from the sun so that the windows and a close-in portion of the camera's line-of-sight were in shadow. The environment around the vehicle at this time appears to be very "clean" with no light scattering particles noticeable.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Dong, Ruobing; Fung, Jeffrey; Chiang, Eugene, E-mail: rdong2013@berkeley.edu
2016-07-20
Direct imaging observations of protoplanetary disks at near-infrared (NIR) wavelengths have revealed structures of potentially planetary origin. Investigations of observational signatures from planet-induced features have so far focused on disks viewed face-on. Combining 3D hydrodynamics and radiative transfer simulations, we study how the appearance of the spiral arms and the gap produced in a disk by a companion varies with inclination and position angle in NIR scattered light. We compare the cases of a 3 M {sub J} and a 0.1 M {sub ⊙} companion, and make predictions suitable for testing with Gemini/GPI, Very Large Telescope/NACO/SPHERE, and Subaru/HiCIAO/SCExAO. We findmore » that the two trailing arms produced by an external perturber can have a variety of morphologies in inclined systems—they may appear as one trailing arm; two trailing arms on the same side of the disk; or two arms winding in opposite directions. The disk ring outside a planetary gap may also mimic spiral arms when viewed at high inclinations. We suggest potential explanations for the features observed in HH 30, HD 141569 A, AK Sco, HD 100546, and AB Aur. We emphasize that inclined views of companion-induced features cannot be converted into face-on views using simple and commonly practiced image deprojections.« less
Generating Color from Polydisperse, Near Micron-Sized TiO2 Particles.
Alam, Al-Mahmnur; Baek, Kyungnae; Son, Jieun; Pei, Yi-Rong; Kim, Dong Ha; Choy, Jin-Ho; Hyun, Jerome K
2017-07-19
Single particle Mie calculations of near micron-sized TiO 2 particles predict strong light scattering dominating the visible range that would give rise to a white appearance. We demonstrate that a polydisperse collection of these "white" particles can result in the generation of visible colors through ensemble scattering. The weighted averaging of the scattering over the particle size distribution modifies the sharp, multiple, high order scattering modes from individual particles into broad variations in the collective extinction. These extinction variations are apparent as visible colors for particles suspended in organic solvent at low concentration, or for a monolayer of particles supported on a transparent substrate viewed in front of a white light source. We further exploit the color variations on optical sensitivity to the surrounding environment to promote micron-sized TiO 2 particles as stable and robust agents for detecting the optical index of homogeneous media with high contrast sensitivities. Such distribution-modulated scattering properties provide TiO 2 particles an intriguing opportunity to impart color and optical sensitivity to their widespread electronic and chemical platforms such as antibacterial windows, catalysis, photocatalysis, optical sensors, and photovoltaics.
NASA Astrophysics Data System (ADS)
Carter, Anna; McCandliss, Stephan R.; Redwine, Keith; Pelton, Russell
2017-01-01
At 23:52 on 17 December 2015 FORTIS (36.312 UG) was launched from the White Sands Missile Range in New Mexico on a mission to observe hydrogen emission and absorption features superimposed on the stellar continua of hot star formation regions in the spiral galaxy NGC 1365. Unfortunately scattered geocoronal Lyα from well outside our nominal (1/2 degree)2 field-of-view (FOV) overwhelmed the signal from the target. Post-flight analysis of the observed scattered light levels, in comparison to a wide-FOV model of the light scattered from various surfaces in the optical train, produced good agreement. Suppression of this scatter is the highest priority for the FORTIS return to flight effort. Our analysis pointed to the need for development of a Wide Field Lyα Geocoronal Simulator (WFLaGS) with a 10○ FOV to fully characterize the end-to-end response of FORTIS to off-axis illumination. Previous end-to-end testing was performed with a vacuum UV collimator with only a limited FOV, ≈ 100”. The development of WFLaGS will allow us to validate our scattered light model and verify our mitigation strategies, which will incorporate low scatter materials, and possibly 3-d printed light traps, covering exposed scatter centers. The design of WFLaGs, consisting of a 50mm diameter F/1 aluminum parabolic collimator and a hydrogen discharge lamp with an ~ 80mm clear MgF2 window is described, along with our initial turn-on tests. This work is supported by NASA grants to John Hopkins University, NNX11AG54G and NNX14AI78G.
First results from the Thomson scattering diagnostic on proto-MPEX.
Biewer, T M; Meitner, S; Rapp, J; Ray, H; Shaw, G
2016-11-01
A Thomson scattering (TS) diagnostic has been successfully implemented on the prototype Material Plasma Exposure eXperiment (Proto-MPEX) at Oak Ridge National Laboratory. The diagnostic collects the light scattered by plasma electrons and spectroscopically resolves the Doppler shift imparted to the light by the velocity of the electrons. The spread in velocities is proportional to the electron temperature, while the total number of photons is proportional to the electron density. TS is a technique used on many devices to measure the electron temperature (T e ) and electron density (n e ) of the plasma. A challenging aspect of the technique is to discriminate the small number of Thomson scattered photons against the large peak of background photons from the high-power laser used to probe the plasma. A variety of methods are used to mitigate the background photons in Proto-MPEX, including Brewster angled windows, viewing dumps, and light baffles. With these methods, first results were measured from argon plasmas in Proto-MPEX, indicating T e ∼ 2 eV and n e ∼ 1 × 10 19 m -3 . The configuration of the Proto-MPEX TS diagnostic will be described and plans for improvement will be given.
NASA Astrophysics Data System (ADS)
Kilaru, M. K.; Cumby, B.; Heikenfeld, J.
2009-01-01
Corner cube and spherical retroreflectors are ubiquitous in conspicuity and range-finding applications since they reflect light back to the illumination source with unmatched efficiency. We report here a switchable electrowetting retroreflector platform that provides multiple novel features, including (a) using <0.5 μJ/cm2 electrical energy to switch from a light scattering state, (b) low loss and wide spectrum as limited only by the absorption spectrum of water, (c) use of ultrasimple self-assembly of 103-105 liquid lenslets/in.2 on a polymer/Al corner-cube substrate, and (d) change in retroreflected irradiance of >10:1 over a ±30° field of view.
Characteristics of color optical shutter with dye-doped polymer network liquid crystal.
Lee, G H; Hwang, K Y; Jang, J E; Jin, Y W; Lee, S Y; Jung, J E
2011-03-01
The optical properties and the theoretical prediction of color optical shutter with dye-doped polymer network liquid crystal (PNLC) were investigated. The view-angle dependence of reflectance according to the bias conditions showed distinctive characteristics, which could be explained from the effects of dye absorption and path length. It was also shown that the thickness dependence of reflectance was strongly influenced by the light-scattering coefficient. Our experimental results matched up well with the theoretical prediction based on the light scattering of liquid crystals in polymer network and the absorption of dichroic dye. This work indicates potential to improve the optical device using dye-doped liquid crystal-polymer composite.
NASA Astrophysics Data System (ADS)
Bozhenkov, S. A.; Beurskens, M.; Dal Molin, A.; Fuchert, G.; Pasch, E.; Stoneking, M. R.; Hirsch, M.; Höfel, U.; Knauer, J.; Svensson, J.; Trimino Mora, H.; Wolf, R. C.
2017-10-01
The optimized stellarator Wendelstein 7-X started operation in December 2015 with a 10 week limiter campaign. Divertor experiments will begin in the second half of 2017. The W7-X Thomson scattering system is an essential diagnostic for electron density and temperature profiles. In this paper the Thomson scattering diagnostic is described in detail, including its design, calibration, data evaluation and first experimental results. Plans for further development are also presented. The W7-X Thomson system is a Nd:YAG setup with up to five lasers, two sets of light collection lenses viewing the entire plasma cross-section, fiber bundles and filter based polychromators. To reduce hardware costs, two or three scattering volumes are measured with a single polychromator. The relative spectral calibration is carried out with the aid of a broadband supercontinuum light source. The absolute calibration is performed by observing Raman scattering in nitrogen. The electron temperatures and densities are recovered by Bayesian modelling. In the first campaign, the diagnostic was equipped for 10 scattering volumes. It provided temperature profiles comparable to those measured using an electron cyclotron emission diagnostic and line integrated densities within 10% of those from a dispersion interferometer.
Atmospheric scattering effects on ground-based measurements of thermospheric winds
NASA Technical Reports Server (NTRS)
Abreu, V. J.; Schmitt, G. A.; Hays, P. B.; Meriwether, J. W., Jr.; Tepley, C. A.; Cogger, L. L.
1983-01-01
Convergent or divergent thermospheric wind patterns detected by ground-based Fabry-Perot interferometric measurements of the Doppler shifts of atomic lines are demonstrated to occur in the presence of strong intensity gradients and a scattering atmosphere. Consideration is given to the color shifts observed when sighting to the north or the south, and a numerical model is developed to describe the wind patterns which produce the recorded shifts. An account is taken of the direct and scattered components of the brightness, with the atmosphere treated as a single scattering layer with a reflecting surface underneath. A scattering coefficient is calculated, together with the line shape of the wavelength shifts. The scattered light is demonstrated, both through simulations and measurements taken near Calgary, Alberta, to produce convergence or divergence of the color shifts, depending on the line-of-sight of the viewing.
Wilson, Robert H; Crouzet, Christian; Torabzadeh, Mohammad; Bazrafkan, Afsheen; Farahabadi, Maryam H; Jamasian, Babak; Donga, Dishant; Alcocer, Juan; Zaher, Shuhab M; Choi, Bernard; Akbari, Yama; Tromberg, Bruce J
2017-10-01
Quantifying rapidly varying perturbations in cerebral tissue absorption and scattering can potentially help to characterize changes in brain function caused by ischemic trauma. We have developed a platform for rapid intrinsic signal brain optical imaging using macroscopically structured light. The device performs fast, multispectral, spatial frequency domain imaging (SFDI), detecting backscattered light from three-phase binary square-wave projected patterns, which have a much higher refresh rate than sinusoidal patterns used in conventional SFDI. Although not as fast as "single-snapshot" spatial frequency methods that do not require three-phase projection, square-wave patterns allow accurate image demodulation in applications such as small animal imaging where the limited field of view does not allow single-phase demodulation. By using 655, 730, and 850 nm light-emitting diodes, two spatial frequencies ([Formula: see text] and [Formula: see text]), three spatial phases (120 deg, 240 deg, and 360 deg), and an overall camera acquisition rate of 167 Hz, we map changes in tissue absorption and reduced scattering parameters ([Formula: see text] and [Formula: see text]) and oxy- and deoxyhemoglobin concentration at [Formula: see text]. We apply this method to a rat model of cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) to quantify hemodynamics and scattering on temporal scales ([Formula: see text]) ranging from tens of milliseconds to minutes. We observe rapid concurrent spatiotemporal changes in tissue oxygenation and scattering during CA and following CPR, even when the cerebral electrical signal is absent. We conclude that square-wave SFDI provides an effective technical strategy for assessing cortical optical and physiological properties by balancing competing performance demands for fast signal acquisition, small fields of view, and quantitative information content.
LIGHT SCATTERING STUDY OF MICRON-SIZE LIQUID AEROSOLS UNDER PULSED CO2 LASER IRRADIATION. (R826767)
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...
New light-shielding technique for shortening the baffle length of a star sensor
NASA Astrophysics Data System (ADS)
Kawano, Hiroyuki; Sato, Yukio; Mitani, Kenji; Kanai, Hiroshi; Hama, Kazumori
2002-10-01
We have developed a star sensor with a short baffle of 140 mm. Our baffle provides a Sun rejection angle of 35 degrees with stray light attenuation less than the intensity level of a visual magnitude of Mv = +5 for a wide field of view lens of 13x13 degrees. The application of a new light shielding technique taking advantage of total internal reflection phenomena enables us to reduce the baffle length to about three fourths that of the conventional two-stage baffle. We have introduced two ideas to make the baffle length shorter. The one is the application of a nearly half sphere convex lens as the first focusing lens. The bottom surface reflects the scattering rays with high incident angles of over 50 degrees by using the total internal reflection phenomena. The other is the painting of the surface of the baffle with not frosted but gloss black paint. The gloss black paint enables most of the specular reflection rays to go back to outer space without scattering. We confirm the baffle performance mentioned above by scattering ray tracing simulation and a light attenuation experiment in a darkroom on the ground.
Here Be Dragons: Characterization of ACS/WFC Scattered Light Anomalies
NASA Astrophysics Data System (ADS)
Porterfield, B.; Coe, D.; Gonzaga, S.; Anderson, J.; Grogin, N.
2016-11-01
We present a study characterizing scattered light anomalies that occur near the edges of Advanced Camera for Surveys (ACS) Wide Field Channel (WFC) images. We inspected all 8,573 full-frame ACS/WFC raw images with exposure times longer than 350 seconds obtained in the F606W and F814W filters from 2002 to October 2013. We visually identified two particular scattered light artifacts known as "dragon's breath" and edge glow. Using the 2MASS point source catalog and Hubble Guide Star Catalog (GSC II), we identified the stars that caused these artifacts. The stars are all located in narrow bands ( 3" across) just outside the ACS/WFC field of view (2" - 16" away). We provide a map of these risky areas around the ACS/WFC detectors - users should avoid positioning bright stars in these regions when designing ACS/WFC imaging observations. We also provide interactive webpages which display all the image artifacts we identified, allowing users to see examples of the severity of artifacts they might expect for a given stellar magnitude at a given position relative to the ACS/WFC field of view. On average, 10th (18th) magnitude stars produce artifacts about 1,000 (100) pixels long. But the severity of these artifacts can vary strongly with small positional shifts (∼ 1"). The results are similar for both filters (F606W and F814W) when expressed in total fluence, or flux multiplied by exposure time.
High-Contrast Near-Infrared Imaging Polarimetry of the Protoplanetary Disk around RY Tau
NASA Technical Reports Server (NTRS)
Takami, Michihiro; Karr, Jennifer L.; Hashimoto, Jun; Kim, Hyosun; Wisenewski, John; Henning, Thomas; Grady, Carol; Kandori, Ryo; Hodapp, Klaus W.; Kudo, Tomoyuki;
2013-01-01
We present near-infrared coronagraphic imaging polarimetry of RY Tau. The scattered light in the circumstellar environment was imaged at H-band at a high resolution (approx. 0.05) for the first time, using Subaru-HiCIAO. The observed polarized intensity (PI) distribution shows a butterfly-like distribution of bright emission with an angular scale similar to the disk observed at millimeter wavelengths. This distribution is offset toward the blueshifted jet, indicating the presence of a geometrically thick disk or a remnant envelope, and therefore the earliest stage of the Class II evolutionary phase. We perform comparisons between the observed PI distribution and disk models with: (1) full radiative transfer code, using the spectral energy distribution (SED) to constrain the disk parameters; and (2) monochromatic simulations of scattered light which explore a wide range of parameters space to constrain the disk and dust parameters. We show that these models cannot consistently explain the observed PI distribution, SED, and the viewing angle inferred by millimeter interferometry. We suggest that the scattered light in the near-infrared is associated with an optically thin and geometrically thick layer above the disk surface, with the surface responsible for the infrared SED. Half of the scattered light and thermal radiation in this layer illuminates the disk surface, and this process may significantly affect the thermal structure of the disk.
Fast imaging of live organisms with sculpted light sheets
NASA Astrophysics Data System (ADS)
Chmielewski, Aleksander K.; Kyrsting, Anders; Mahou, Pierre; Wayland, Matthew T.; Muresan, Leila; Evers, Jan Felix; Kaminski, Clemens F.
2015-04-01
Light-sheet microscopy is an increasingly popular technique in the life sciences due to its fast 3D imaging capability of fluorescent samples with low photo toxicity compared to confocal methods. In this work we present a new, fast, flexible and simple to implement method to optimize the illumination light-sheet to the requirement at hand. A telescope composed of two electrically tuneable lenses enables us to define thickness and position of the light-sheet independently but accurately within milliseconds, and therefore optimize image quality of the features of interest interactively. We demonstrated the practical benefit of this technique by 1) assembling large field of views from tiled single exposure each with individually optimized illumination settings; 2) sculpting the light-sheet to trace complex sample shapes within single exposures. This technique proved compatible with confocal line scanning detection, further improving image contrast and resolution. Finally, we determined the effect of light-sheet optimization in the context of scattering tissue, devising procedures for balancing image quality, field of view and acquisition speed.
Conjugate adaptive optics with remote focusing in multiphoton microscopy
NASA Astrophysics Data System (ADS)
Tao, Xiaodong; Lam, Tuwin; Zhu, Bingzhao; Li, Qinggele; Reinig, Marc R.; Kubby, Joel
2018-02-01
The small correction volume for conventional wavefront shaping methods limits their application in biological imaging through scattering media. In this paper, we take advantage of conjugate adaptive optics (CAO) and remote focusing (CAORF) to achieve three-dimensional (3D) scanning through a scattering layer with a single correction. Our results show that the proposed system can provide 10 times wider axial field of view compared with a conventional conjugate AO system when 16,384 segments are used on a spatial light modulator. We demonstrate two-photon imaging with CAORF through mouse skull. The fluorescent microspheres embedded under the scattering layers can be clearly observed after applying the correction.
The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...
NASA Astrophysics Data System (ADS)
Ding, Lei; Wang, Lu-Wei; Zhou, Lei; Zhang, Fang-hui
2016-12-01
An out-coupling membrane embedded with a scattering film of SiO2 spheres and polyethylene terephthalate (PET) plastic was successfully developed for 150 × 150 mm2 green OLEDs. Comparing with a reference OLED panel, an approximately 1-fold enhancement in the forward emission was obtained with an out-coupling membrane adhered to the surface of the external glass substrate of the panel. Moreover, it was verified that the emission color at different viewing angles can be stabilized without apparent spectral distortion. Particularly, the uniformity of the large-area OLEDs was greatly improved. Theoretical calculation clarified that the improved performance of the lighting panels is primarily attributed to the effect of particle scattering.
Coherent beam control through inhomogeneous media in multi-photon microscopy
NASA Astrophysics Data System (ADS)
Paudel, Hari Prasad
Multi-photon fluorescence microscopy has become a primary tool for high-resolution deep tissue imaging because of its sensitivity to ballistic excitation photons in comparison to scattered excitation photons. The imaging depth of multi-photon microscopes in tissue imaging is limited primarily by background fluorescence that is generated by scattered light due to the random fluctuations in refractive index inside the media, and by reduced intensity in the ballistic focal volume due to aberrations within the tissue and at its interface. We built two multi-photon adaptive optics (AO) correction systems, one for combating scattering and aberration problems, and another for compensating interface aberrations. For scattering correction a MEMS segmented deformable mirror (SDM) was inserted at a plane conjugate to the objective back-pupil plane. The SDM can pre-compensate for light scattering by coherent combination of the scattered light to make an apparent focus even at a depths where negligible ballistic light remains (i.e. ballistic limit). This problem was approached by investigating the spatial and temporal focusing characteristics of a broad-band light source through strongly scattering media. A new model was developed for coherent focus enhancement through or inside the strongly media based on the initial speckle contrast. A layer of fluorescent beads under a mouse skull was imaged using an iterative coherent beam control method in the prototype two-photon microscope to demonstrate the technique. We also adapted an AO correction system to an existing in three-photon microscope in a collaborator lab at Cornell University. In the second AO correction approach a continuous deformable mirror (CDM) is placed at a plane conjugate to the plane of an interface aberration. We demonstrated that this "Conjugate AO" technique yields a large field-of-view (FOV) advantage in comparison to Pupil AO. Further, we showed that the extended FOV in conjugate AO is maintained over a relatively large axial misalignment of the conjugate planes of the CDM and the aberrating interface. This dissertation advances the field of microscopy by providing new models and techniques for imaging deeply within strongly scattering tissue, and by describing new adaptive optics approaches to extending imaging FOV due to sample aberrations.
NASA Technical Reports Server (NTRS)
Fymat, A. L.
1976-01-01
The paper studies the inversion of the radiative transfer equation describing the interaction of electromagnetic radiation with atmospheric aerosols. The interaction can be considered as the propagation in the aerosol medium of two light beams: the direct beam in the line-of-sight attenuated by absorption and scattering, and the diffuse beam arising from scattering into the viewing direction, which propagates more or less in random fashion. The latter beam has single scattering and multiple scattering contributions. In the former case and for single scattering, the problem is reducible to first-kind Fredholm equations, while for multiple scattering it is necessary to invert partial integrodifferential equations. A nonlinear minimization search method, applicable to the solution of both types of problems has been developed, and is applied here to the problem of monitoring aerosol pollution, namely the complex refractive index and size distribution of aerosol particles.
First results from the Thomson scattering diagnostic on proto-MPEX
DOE Office of Scientific and Technical Information (OSTI.GOV)
Biewer, T. M., E-mail: biewertm@ornl.gov; Meitner, S.; Rapp, J.
2016-11-15
A Thomson scattering (TS) diagnostic has been successfully implemented on the prototype Material Plasma Exposure eXperiment (Proto-MPEX) at Oak Ridge National Laboratory. The diagnostic collects the light scattered by plasma electrons and spectroscopically resolves the Doppler shift imparted to the light by the velocity of the electrons. The spread in velocities is proportional to the electron temperature, while the total number of photons is proportional to the electron density. TS is a technique used on many devices to measure the electron temperature (T{sub e}) and electron density (n{sub e}) of the plasma. A challenging aspect of the technique is tomore » discriminate the small number of Thomson scattered photons against the large peak of background photons from the high-power laser used to probe the plasma. A variety of methods are used to mitigate the background photons in Proto-MPEX, including Brewster angled windows, viewing dumps, and light baffles. With these methods, first results were measured from argon plasmas in Proto-MPEX, indicating T{sub e} ∼ 2 eV and n{sub e} ∼ 1 × 10{sup 19} m{sup −3}. The configuration of the Proto-MPEX TS diagnostic will be described and plans for improvement will be given.« less
Color structured light imaging of skin
NASA Astrophysics Data System (ADS)
Yang, Bin; Lesicko, John; Moy, Austin; Reichenberg, Jason; Sacks, Michael; Tunnell, James W.
2016-05-01
We illustrate wide-field imaging of skin using a structured light (SL) approach that highlights the contrast from superficial tissue scattering. Setting the spatial frequency of the SL in a regime that limits the penetration depth effectively gates the image for photons that originate from the skin surface. Further, rendering the SL images in a color format provides an intuitive format for viewing skin pathologies. We demonstrate this approach in skin pathologies using a custom-built handheld SL imaging system.
NASA Astrophysics Data System (ADS)
Sun, Zhongqiu; Peng, Zhiyan; Wu, Di; Lv, Yunfeng
2018-02-01
The optical scattering property of the target is the essential signal for passive remote sensing applications. To deepen our understanding of the light reflected from vegetation, we present results of photopolarimetric laboratory measurements from single leaf and two vegetation covers (planophile and erectophile) over a wide range of viewing directions. The bidirectional polarized reflectance factor (BPRF) was used to characterize the polarization property of our samples. We observed positive and negative polarization (-BPRFQ) of all samples in the forward scattering and backward scattering directions, respectively. Based on the comparison of the BPRF among single leaf, planophile vegetation and erectophile vegetation, our measurements demonstrate that the orientation of the leaf is a key factor in describing the amount of polarization in the forward scattering direction. Our measurements also validated certain model results stating that (1) specular reflection generates a portion of polarization in the forward scattering direction and diffuses scattering of polarized light in all hemisphere directions, (2) BPRFU is anti-symmetric in the principal plane from a recent study in which the authors simulated the polarized reflectance of vegetation cover using the vector radiative transfer theory. These photopolarimetric measurement results, which can be completely explained by the theoretical results, are useful in remote sensing applications to vegetation.
NASA Technical Reports Server (NTRS)
Fymat, A. L.
1975-01-01
Instrument is based on inverse solution ot equations for light scattered by a transparent medium. Measurements are taken over several angles of incidence rather than over several frequencies. Measurements can be used to simultaneously determine chemical and physical properties of particles in mixed gas or liquid.
FDTD analysis of the light extraction efficiency of OLEDs with a random scattering layer.
Kim, Jun-Whee; Jang, Ji-Hyang; Oh, Min-Cheol; Shin, Jin-Wook; Cho, Doo-Hee; Moon, Jae-Hyun; Lee, Jeong-Ik
2014-01-13
The light extraction efficiency of OLEDs with a nano-sized random scattering layer (RSL-OLEDs) was analyzed using the Finite Difference Time Domain (FDTD) method. In contrast to periodic diffraction patterns, the presence of an RSL suppresses the spectral shift with respect to the viewing angle. For FDTD simulation of RSL-OLEDs, a planar light source with a certain spatial and temporal coherence was incorporated, and the light extraction efficiency with respect to the fill factor of the RSL and the absorption coefficient of the material was investigated. The design results were compared to the experimental results of the RSL-OLEDs in order to confirm the usefulness of FDTD in predicting experimental results. According to our FDTD simulations, the light confined within the ITO-organic waveguide was quickly absorbed, and the absorption coefficients of ITO and RSL materials should be reduced in order to obtain significant improvement in the external quantum efficiency (EQE). When the extinction coefficient of ITO was 0.01, the EQE in the RSL-OLED was simulated to be enhanced by a factor of 1.8.
A JOINT CHANDRA AND SWIFT VIEW OF THE 2015 X-RAY DUST-SCATTERING ECHO OF V404 CYGNI
DOE Office of Scientific and Technical Information (OSTI.GOV)
Heinz, S.; Corrales, L.; Neilsen, J.
2016-07-01
We present a combined analysis of the Chandra and Swift observations of the 2015 X-ray echo of V404 Cygni. Using a stacking analysis, we identify eight separate rings in the echo. We reconstruct the soft X-ray light curve of the 2015 June outburst using the high-resolution Chandra images and cross-correlations of the radial intensity profiles, indicating that about 70% of the outburst fluence occurred during the bright flare at the end of the outburst on MJD 57199.8. By deconvolving the intensity profiles with the reconstructed outburst light curve, we show that the rings correspond to eight separate dust concentrations withmore » precise distance determinations. We further show that the column density of the clouds varies significantly across the field of view, with the centroid of most of the clouds shifted toward the Galactic plane, relative to the position of V404 Cyg, invalidating the assumption of uniform cloud column typically made in attempts to constrain dust properties from light echoes. We present a new XSPEC spectral dust-scattering model that calculates the differential dust-scattering cross section for a range of commonly used dust distributions and compositions and use it to jointly fit the entire set of Swift echo data. We find that a standard Mathis–Rumpl–Nordsieck model provides an adequate fit to the ensemble of echo data. The fit is improved by allowing steeper dust distributions, and models with simple silicate and graphite grains are preferred over models with more complex composition.« less
KUMAR, ABHISHEK; CHRISTENSEN, RYAN; GUO, MIN; CHANDRIS, PANOS; DUNCAN, WILLIAM; WU, YICONG; SANTELLA, ANTHONY; MOYLE, MARK; WINTER, PETER W.; COLÓN-RAMOS, DANIEL; BAO, ZHIRONG; SHROFF, HARI
2017-01-01
Dual-view inverted selective plane illumination microscopy (diSPIM) enables high-speed, long-term, fourdimensional (4D) imaging with isotropic spatial resolution. It is also compatible with conventional sample mounting on glass coverslips. However, broadening of the light sheet at distances far from the beam waist and sample-induced scattering degrades diSPIM contrast and optical sectioning. We describe two simple improvements that address both issues and entail no additional hardware modifications to the base diSPIM. First, we demonstrate improved diSPIM sectioning by keeping the light sheet and detection optics stationary, and scanning the sample through the stationary light sheet (rather than scanning the broadening light sheet and detection plane through the stationary sample, as in conventional diSPIM). This stage-scanning approach allows a thinner sheet to be used when imaging laterally extended samples, such as fixed microtubules or motile mitochondria in cell monolayers, and produces finer contrast than does conventional diSPIM. We also used stage-scanning diSPIM to obtain high-quality, 4D nuclear datasets derived from an uncompressed nematode embryo, and performed lineaging analysis to track 97% of cells until twitching. Second, we describe the improvement of contrast in thick, scattering specimens by synchronizing light-sheet synthesis with the rolling, electronic shutter of our scientific complementary metal-oxide-semiconductor (sCMOS) detector. This maneuver forms a virtual confocal slit in the detection path, partially removing out-of-focus light. We demonstrate the applicability of our combined stage- and slit-scanning-methods by imaging pollen grains and nuclear and neuronal structures in live nematode embryos. All acquisition and analysis code is freely available online. PMID:27638693
Phoenix's Laser Beam in Action on Mars
NASA Technical Reports Server (NTRS)
2008-01-01
[figure removed for brevity, see original site] Click on image to view the animation The Surface Stereo Imager camera aboard NASA's Phoenix Mars Lander acquired a series of images of the laser beam in the Martian night sky. Bright spots in the beam are reflections from ice crystals in the low level ice-fog. The brighter area at the top of the beam is due to enhanced scattering of the laser light in a cloud. The Canadian-built lidar instrument emits pulses of laser light and records what is scattered back. The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.Multiple light scattering in metallic ejecta produced under intense shockwave compression.
Franzkowiak, J-E; Mercier, P; Prudhomme, G; Berthe, L
2018-04-10
A roughened metallic plate, subjected to intense shock wave compression, gives rise to an expanding ejecta particle cloud. Photonic Doppler velocimetry (PDV), a fiber-based heterodyne velocimeter, is often used to track ejecta velocities in dynamic compression experiments and on nanosecond time scales. Shortly after shock breakout at the metal-vacuum interface, a particular feature observed in many experiments in the velocity spectrograms is what appear to be slow-moving ejecta, below the free-surface velocity. Using Doppler Monte Carlo simulations incorporating the transport of polarization in the ejecta, we show that this feature is likely to be explained by the multiple scattering of light, rather than by possible collisions among particles, slowing down the ejecta. As the cloud expands in a vacuum, the contribution of multiple scattering decreases due to the limited field of view of the pigtailed collimator used to probe the ejecta, showing that the whole geometry of the system must be taken into account in the calculations to interpret and predict PDV measurements.
Developing a more useful surface quality metric for laser optics
NASA Astrophysics Data System (ADS)
Turchette, Quentin; Turner, Trey
2011-02-01
Light scatter due to surface defects on laser resonator optics produces losses which lower system efficiency and output power. The traditional methodology for surface quality inspection involves visual comparison of a component to scratch and dig (SAD) standards under controlled lighting and viewing conditions. Unfortunately, this process is subjective and operator dependent. Also, there is no clear correlation between inspection results and the actual performance impact of the optic in a laser resonator. As a result, laser manufacturers often overspecify surface quality in order to ensure that optics will not degrade laser performance due to scatter. This can drive up component costs and lengthen lead times. Alternatively, an objective test system for measuring optical scatter from defects can be constructed with a microscope, calibrated lighting, a CCD detector and image processing software. This approach is quantitative, highly repeatable and totally operator independent. Furthermore, it is flexible, allowing the user to set threshold levels as to what will or will not constitute a defect. This paper details how this automated, quantitative type of surface quality measurement can be constructed, and shows how its results correlate against conventional loss measurement techniques such as cavity ringdown times.
2011-12-19
NASA acquired November 24, 2011 From its vantage 824 kilometers (512 miles) above Earth, the Visible Infrared Imager Radiometer Suite (VIIRS) on the NPOESS Preparatory Project (NPP) satellite gets a complete view of our planet every day. This image from November 24, 2011, is the first complete global image from VIIRS. The NPP satellite launched on October 28, 2011, and VIIRS acquired its first measurements on November 21. To date, the images are preliminary, used to gauge the health of the sensor as engineers continue to power it up for full operation. Rising from the south and setting in the north on the daylight side of Earth, VIIRS images the surface in long wedges measuring 3,000 kilometers (1,900 miles) across. The swaths from each successive orbit overlap one another, so that at the end of the day, the sensor has a complete view of the globe. The Arctic is missing because it is too dark to view in visible light during the winter. The NPP satellite was placed in a Sun-synchronous orbit, a unique path that takes the satellite over the equator at the same local (ground) time in every orbit. So, when NPP flies over Kenya, it is about 1:30 p.m. on the ground. When NPP reaches Gabon—about 3,000 kilometers to the west—on the next orbit, it is close to 1:30 p.m. on the ground. This orbit allows the satellite to maintain the same angle between the Earth and the Sun so that all images have similar lighting. The consistent lighting is evident in the daily global image. Stripes of sunlight (sunglint) reflect off the ocean in the same place on the left side of every swath. The consistent angle is important because it allows scientists to compare images from year to year without worrying about extreme changes in shadows and lighting. The image also shows a band of haze along the right side of every orbit swath. When light travels through the atmosphere, it bounces off particles or scatters, making the atmosphere look hazy. The scattering effect is most pronounced along the edge of the swath, where the sensor is looking at an angle through more of the atmosphere. Scientists can correct for this scattering effect, but need measurements from a range of wavelengths to do so. The degree to which light scatters depends partly on the wavelength of the light. Blue light scatters more than red light, for example, which is why the sky is blue. VIIRS measures 22 different wavelengths of light, but not all of the sensor’s detectors are operating at peak performance yet. Those measuring thermal infrared light are not yet cold enough to collect reliable measurements. Once VIIRS begins full operations, it will produce a range of measurements from ocean temperature to clouds to the locations of fires. These measurements will help extend the record from earlier sensors like the Moderate Resolution Imaging Spectroradiometer (MODIS). VIIRS is very similar to MODIS, but flies at a higher altitude to measure the whole planet without gaps. (MODIS daily measurements have gaps at the equator. See the MODIS image from November 24.) VIIRS also sees the Earth in less detail, 375 meters per pixel, compared to 250 meters per pixel for MODIS. Image by NASA’s NPP Land Product Evaluation and Testing Element. Caption by Holli Riebeek. Credit: NASA Earth Observatory NASA image use policy. NASA Goddard Space Flight Center enables NASA’s mission through four scientific endeavors: Earth Science, Heliophysics, Solar System Exploration, and Astrophysics. Goddard plays a leading role in NASA’s accomplishments by contributing compelling scientific knowledge to advance the Agency’s mission. Follow us on Twitter Like us on Facebook Find us on Instagram
Earth Observations taken by the Expedition 27 Crew
2011-04-12
ISS027-E-012224 (12 April 2011) --- Sunset over western South America is featured in this image photographed by an Expedition 27 crew member on the International Space Station. Crew members onboard the space station see, on average, sixteen sunrises and sunsets during a 24-hour orbital period. Each changeover between day and night on the ground is marked by the terminator, or line separating the sunlit side of Earth from the side in darkness. While the terminator is conceptualized as a hard boundary?and is frequently presented as such in graphics and visualizations?in reality the boundary between light and dark is diffuse due to scattering of light by Earth?s atmosphere. This zone of diffuse lighting is experienced as dusk or twilight on the ground ? while the sun is no longer visible, some illumination is still present due to light scattering over the local horizon. The terminator is visible in this photograph trending across the image from lower left to upper right. This panoramic view across central South America, looking towards the northeast, was acquired at approximately 7:37 p.m. local time. Layers of Earth?s atmosphere, colored bright white to deep blue, are visible extending across the horizon (or limb). The highest cloud tops have a reddish glow from the direct light of the setting sun while lower clouds are in twilight. The Salar de Coipasa, a large salt lake in Bolivia, is dimly visible on the night side of the terminator. The salar provides a geographic reference point that allows the location and viewing orientation of the image to be determined.
Gedzelman, Stanley David
2017-07-01
Three scenarios that produce colored thunderstorms are simulated. In Scenario #1, the thunderstorm's sunlit face exhibits a color gradient from white or yellow at top to red at base when the sun is near the horizon. It is simulated with a second-order scattering model as a combination of sunlight and skylight reflected from the cloud face that is attenuated and reddened by Rayleigh and Mie scattering over the long optical path near sunset that increases from cloud top to base. In Scenario #2, the base of the precipitation shaft appears luminous green-blue when surrounded by a much darker arcus cloud. It is simulated as multiply scattered light transmitted through the precipitation shaft using a Monte Carlo model that includes absorption by liquid water and ice. The color occurs over a wide range of solar zenith angles with large liquid water content, but the precipitation shaft is only bright when hydrometeors are large. Attenuation of the light by Rayleigh and Mie scattering outside the precipitation shaft shifts the spectrum to green when viewed from a distance of several kilometers. In Scenario #3, the shaded cloud face exhibits a "sickly" yellow-green color. It is simulated with a second-order scattering model as the result of distant skylight that originates in the sunlit region beyond an opaque anvil of order 40 km wide but is attenuated by Rayleigh and Mie scattering in its path to the cloud and observer.
A new technique for the measurement of surface shear stress vectors using liquid crystal coatings
NASA Technical Reports Server (NTRS)
Reda, Daniel C.; Muratore, J. J., Jr.
1994-01-01
Research has recently shown that liquid crystal coating (LCC) color-change response to shear depends on both shear stress magnitude and direction. Additional research was thus conducted to extend the LCC method from a flow-visualization tool to a surface shear stress vector measurement technique. A shear-sensitive LCC was applied to a planar test surface and illuminated by white light from the normal direction. A fiber optic probe was used to capture light scattered by the LCC from a point on the centerline of a turbulent, tangential-jet flow. Both the relative shear stress magnitude and the relative in-plane view angle between the sensor and the centerline shear vector were systematically varied. A spectrophotometer was used to obtain scattered-light spectra which were used to quantify the LCC color (dominant wavelength) as a function of shear stress magnitude and direction. At any fixed shear stress magnitude, the minimum dominant wavelength was measured when the shear vector was aligned with and directed away from the observer; changes in the relative in-plane view angle to either side of this vector/observer aligned position resulted in symmetric Gaussian increases in measured dominant wavelength. Based on these results, a vector measurement methodology, involving multiple oblique-view observations of the test surface, was formulated. Under present test conditions, the measurement resolution of this technique was found to be +/- 1 deg for vector orientations and +/- 5% for vector magnitudes. An approach t o extend the present methodology to full-surface applications is proposed.
Manakins can produce iridescent and bright feather colours without melanosomes.
Igic, Branislav; D'Alba, Liliana; Shawkey, Matthew D
2016-06-15
Males of many species often use colourful and conspicuous ornaments to attract females. Among these, male manakins (family: Pipridae) provide classic examples of sexual selection favouring the evolution of bright and colourful plumage coloration. The highly iridescent feather colours of birds are most commonly produced by the periodic arrangement of melanin-containing organelles (melanosomes) within barbules. Melanin increases the saturation of iridescent colours seen from optimal viewing angles by absorbing back-scattered light; however, this may reduce the wide-angle brightness of these signals, contributing to a dark background appearance. We examined the nanostructure of four manakin species (Lepidothrix isidorei, L. iris, L. nattereri and L. coeruleocapilla) to identify how they produce their bright plumage colours. Feather barbs of all four species were characterized by dense and fibrous internal spongy matrices that likely increase scattering of light within the barb. The iridescent, yet pale or whitish colours of L. iris and L. nattereri feathers were produced not by periodically arranged melanosomes within barbules, but by periodic matrices of air and β-keratin within barbs. Lepidothrix iris crown feathers were able to produce a dazzling display of colours with small shifts in viewing geometry, likely because of a periodic nanostructure, a flattened barb morphology and disorder at a microstructural level. We hypothesize that iridescent plumage ornaments of male L. iris and L. nattereri are under selection to increase brightness or luminance across wide viewing angles, which may potentially increase their detectability by females during dynamic and fast-paced courtship displays in dim light environments. © 2016. Published by The Company of Biologists Ltd.
Thomson scattering density calibration by Rayleigh and rotational Raman scattering on NSTX.
LeBlanc, B P
2008-10-01
The multipoint Thomson scattering diagnostic measures the profiles of the electron temperature T(e)(R) and density n(e)(R) on the horizontal midplane of NSTX. Normal operation makes use of Rayleigh scattering in nitrogen or argon to derive the density profile. While the Rayleigh scattering n(e)(R) calibration has been validated by comparison to other density measurements and through its correlation with plasma phenomena, it does require dedicated detectors at the laser wavelength in this filter polychromator based diagnostic. The presence of dust and/or stray laser light precludes routine use of these dedicated spectral channels for Thomson scattering measurement. Hence it is of interest to investigate the use of Raman scattering in nitrogen for the purpose of density calibration since it could free up detection equipment, which could then be used for the instrumentation of additional radial channels. In this paper the viewing optics "geometrical factor" profiles obtained from Rayleigh and Raman scattering are compared. While both techniques agree nominally, residual effects on the order of 10% remain and will be discussed.
Bessel light sheet structured illumination microscopy
NASA Astrophysics Data System (ADS)
Noshirvani Allahabadi, Golchehr
Biomedical study researchers using animals to model disease and treatment need fast, deep, noninvasive, and inexpensive multi-channel imaging methods. Traditional fluorescence microscopy meets those criteria to an extent. Specifically, two-photon and confocal microscopy, the two most commonly used methods, are limited in penetration depth, cost, resolution, and field of view. In addition, two-photon microscopy has limited ability in multi-channel imaging. Light sheet microscopy, a fast developing 3D fluorescence imaging method, offers attractive advantages over traditional two-photon and confocal microscopy. Light sheet microscopy is much more applicable for in vivo 3D time-lapsed imaging, owing to its selective illumination of tissue layer, superior speed, low light exposure, high penetration depth, and low levels of photobleaching. However, standard light sheet microscopy using Gaussian beam excitation has two main disadvantages: 1) the field of view (FOV) of light sheet microscopy is limited by the depth of focus of the Gaussian beam. 2) Light-sheet images can be degraded by scattering, which limits the penetration of the excitation beam and blurs emission images in deep tissue layers. While two-sided sheet illumination, which doubles the field of view by illuminating the sample from opposite sides, offers a potential solution, the technique adds complexity and cost to the imaging system. We investigate a new technique to address these limitations: Bessel light sheet microscopy in combination with incoherent nonlinear Structured Illumination Microscopy (SIM). Results demonstrate that, at visible wavelengths, Bessel excitation penetrates up to 250 microns deep in the scattering media with single-side illumination. Bessel light sheet microscope achieves confocal level resolution at a lateral resolution of 0.3 micron and an axial resolution of 1 micron. Incoherent nonlinear SIM further reduces the diffused background in Bessel light sheet images, resulting in confocal quality images in thick tissue. The technique was applied to live transgenic zebra fish tg(kdrl:GFP), and the sub-cellular structure of fish vasculature genetically labeled with GFP was captured in 3D. The superior speed of the microscope enables us to acquire signal from 200 layers of a thick sample in 4 minutes. The compact microscope uses exclusively off-the-shelf components and offers a low-cost imaging solution for studying small animal models or tissue samples.
Theoretical evaluation of scattering effect on retroreflective free-space optical communication.
Yin, Hongwei; Lan, Tianpeng; Zhang, Hailiang; Jia, Honghui; Chang, Shengli; Yang, Juncai
2012-12-01
Retroreflective free-space optical (RFSO) communication is a new concept of optical communication; it consists of an optical transceiver and a retromodulator and has advantages such as light weight, small volume, and low power consumption. The power captured by the receiver consists of two parts: retroreflective and scattering. The retroreflective characteristics are obtained using an analytical formula, the scattering characteristics using a Monte Carlo model. Results show that the scattering power plays an important role in a RFSO communication link, especially when the communication range is long or the meteorological range is short. Some rules are also obtained for the sake of system design, which include increasing the range from the transmitter and the receiver properly, increasing the area of the retromodulator, limiting the field of view of the receiver, and limiting the beam divergence of the transmitter.
Optical Sensors Using Stimulated Brillouin Scattering
NASA Technical Reports Server (NTRS)
Christensen, Caleb A (Inventor); Zavriyev, Anton (Inventor)
2017-01-01
A method for enhancing a sensitivity of an optical sensor having an optical cavity counter-propagates beams of pump light within the optical cavity to produce scattered light based on Stimulated Brillouin Scattering (SBS). The properties of the pump light are selected to generate fast-light conditions for the scattered light, such that the scattered light includes counter-propagating beams of fast light. The method prevents the pump light from resonating within the optical cavity, while allowing the scattered light to resonate within the optical cavity. At least portions of the scattered light are interfered outside of the optical cavity to produce a beat note for a measurement of the optical sensor. The disclosed method is particularly applicable to optical gyroscopes.
Laser Speckle Contrast Imaging: theory, instrumentation and applications.
Senarathna, Janaka; Rege, Abhishek; Li, Nan; Thakor, Nitish V
2013-01-01
Laser Speckle Contrast Imaging (LSCI) is a wide field of view, non scanning optical technique for observing blood flow. Speckles are produced when coherent light scattered back from biological tissue is diffracted through the limiting aperture of focusing optics. Mobile scatterers cause the speckle pattern to blur; a model can be constructed by inversely relating the degree of blur, termed speckle contrast to the scatterer speed. In tissue, red blood cells are the main source of moving scatterers. Therefore, blood flow acts as a virtual contrast agent, outlining blood vessels. The spatial resolution (~10 μm) and temporal resolution (10 ms to 10 s) of LSCI can be tailored to the application. Restricted by the penetration depth of light, LSCI can only visualize superficial blood flow. Additionally, due to its non scanning nature, LSCI is unable to provide depth resolved images. The simple setup and non-dependence on exogenous contrast agents have made LSCI a popular tool for studying vascular structure and blood flow dynamics. We discuss the theory and practice of LSCI and critically analyze its merit in major areas of application such as retinal imaging, imaging of skin perfusion as well as imaging of neurophysiology.
Telescope with a wide field of view internal optical scanner
NASA Technical Reports Server (NTRS)
Zheng, Yunhui (Inventor); Degnan, III, John James (Inventor)
2012-01-01
A telescope with internal scanner utilizing either a single optical wedge scanner or a dual optical wedge scanner and a controller arranged to control a synchronous rotation of the first and/or second optical wedges, the wedges constructed and arranged to scan light redirected by topological surfaces and/or volumetric scatterers. The telescope with internal scanner further incorporates a first converging optical element that receives the redirected light and transmits the redirected light to the scanner, and a second converging optical element within the light path between the first optical element and the scanner arranged to reduce an area of impact on the scanner of the beam collected by the first optical element.
Dual-axis confocal microscope for high-resolution in vivo imaging
Wang, Thomas D.; Mandella, Michael J.; Contag, Christopher H.; Kino, Gordon S.
2007-01-01
We describe a novel confocal microscope that uses separate low-numerical-aperture objectives with the illumination and collection axes crossed at angle θ from the midline. This architecture collects images in scattering media with high transverse and axial resolution, long working distance, large field of view, and reduced noise from scattered light. We measured transverse and axial (FWHM) resolution of 1.3 and 2.1 μm, respectively, in free space, and confirm subcellular resolution in excised esophageal mucosa. The optics may be scaled to millimeter dimensions and fiber coupled for collection of high-resolution images in vivo. PMID:12659264
NASA Astrophysics Data System (ADS)
Bullock, J. G.; Ross, D. A.
The fibre optic Doppler anemometer (FODA) has been used to develop an accurate quantitative method of routinely assessing bull fertility. This method is of importance to the artificial insemination industry because the present qualitative estimation, performed by viewing semen using a microscope, can only set broad limits of quality. Laser light from the FODA was directed into diluted semen samples and the back scattered light was measured. A digital correlator was used to calculate the signal correlation of the back scattered light. The resultant data curves were interpreted in terms of the collective motility and swimming speed of the spermatozoa using a microcomputer. These two parameters are accepted as being indicative of fertility. The accuracy of this method is demonstrated by examination of results obtained in an experiment where enzymes, thought to alter fertility, were added to semen. The effect of the enzymes on the swimming speed and motility was clearly demonstrated.
Ocular forward light scattering and corneal backward light scattering in patients with dry eye.
Koh, Shizuka; Maeda, Naoyuki; Ikeda, Chikako; Asonuma, Sanae; Mitamura, Hayato; Oie, Yoshinori; Soma, Takeshi; Tsujikawa, Motokazu; Kawasaki, Satoshi; Nishida, Kohji
2014-09-18
To evaluate ocular forward light scattering and corneal backward light scattering in patients with dry eye. Thirty-five eyes in 35 patients with dry eye and 20 eyes of 20 healthy control subjects were enrolled. The 35 dry eyes were classified into two groups according to whether superficial punctate keratopathy in the central 6-mm corneal zone (cSPK) was present or not. Ocular forward light scattering was quantified with a straylight meter. Corneal backward light scattering from the anterior, middle, and posterior corneal parts was assessed with a corneal densitometry program using the Scheimpflug imaging system. Both dry eye groups had significantly higher intraocular forward light scattering than the control group (both P<0.05). The dry eye group with cSPK had significantly higher values in anterior and total corneal backward light scattering than the other two groups. Moderate positive correlations were observed between the cSPK score and corneal backward light scattering from the anterior cornea (R=0.60, P<0.001) and corneal backward light scattering from the total cornea (R=0.54, P<0.001); however, no correlation was found between cSPK score and ocular forward light scattering (R=0.01, P=0.932). Ocular forward light scattering and corneal backward light scattering from the anterior cornea were greater in dry eyes than in normal eyes. Increased corneal backward light scattering in dry eye at least partially results from cSPK overlying the optical zone. Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.
NASA Astrophysics Data System (ADS)
Strola, S. A.; Schultz, E.; Allier, C. P.; DesRoches, B.; Lemmonier, J.; Dinten, J.-M.
2013-03-01
In this paper, we report on a compact prototype capable both of lensfree imaging, Raman spectrometry and scattering microscopy from bacteria samples. This instrument allows high-throughput real-time characterization without the need of markers, making it potentially suitable to field label-free biomedical and environmental applications. Samples are illuminated from above with a focused-collimated 532nm laser beam and can be x-y-z scanned. The bacteria detection is based on emerging lensfree imaging technology able to localize cells of interest over a large field-of-view of 24mm2. Raman signal and scattered light are then collected by separate measurement arms simultaneously. In the first arm the emission light is fed by a fiber into a prototype spectrometer, developed by Tornado Spectral System based on Tornado's High Throughput Virtual Slit (HTVS) novel technology. The enhanced light throughput in the spectral region of interest (500-1800 cm-1) reduces Raman acquisition time down to few seconds, thus facilitating experimental protocols and avoiding the bacteria deterioration induced by laser thermal heating. Scattered light impinging in the second arm is collected onto a charge-coupled-device. The reconstructed image allows studying the single bacteria diffraction pattern and their specific structural features. The characterization and identification of different bacteria have been performed to validate and optimize the acquisition system and the component setup. The results obtained demonstrate the benefits of these three techniques combination by providing the precise bacteria localization, their chemical composition and a morphology description. The procedure for a rapid identification of particular pathogen bacteria in a sample is illustrated.
2003-11-09
In this lunar eclipse viewed from Merritt Island, Fla., the full moon takes on a dark red color because it is being lighted slightly by sunlight passing through the Earth's atmosphere. This light has the blue component preferentially scattered out (this is also why the sky appears blue from the surface of the Earth), leaving faint reddish light to illuminate the Moon. Eclipses occur when the Sun, Earth and Moon line up. They are rare because the Moon usually passes above or below the imaginary line connecting Earth and the Sun. The Earth casts a shadow that the Moon can pass through - when it does, it is called a lunar eclipse.
NASA Astrophysics Data System (ADS)
Morozov, A.; Krücken, R.; Ulrich, A.; Wieser, J.
2006-11-01
Side-view intensity profiles of fluorescent light were measured for neon and nitrogen excited with 12keV electron beams at gas pressures from 250to1400hPa. The intensity profiles were compared with theoretical profiles calculated using the CASINO program which performs Monte Carlo simulations of electron scattering. It was assumed that the spatial distribution of fluorescent intensity is directly proportional to the spatial distribution of energy loss by primary electrons. The comparison shows good correlation of experimental data and the results of numeric simulations.
A novel screen design for anti-ambient light front projection display with angle-selective absorber
NASA Astrophysics Data System (ADS)
Liao, Tianju; Chen, Weigang; He, Kebo; Zhang, Zhaoyu
2016-03-01
Ambient light is destructive to the reflective type projection system's contrast ratio which has great influence on the image quality. In contrast to the conventional front projection, short-throw projection has its advantage to reject the ambient light. Fresnel lens-shaped reflection layer is adapted to direct light from a large angle due to the low lens throw ratio to the viewing area. The structure separates the path of the ambient light and projection light, creating the chance to solve the problem that ambient light is mixed with projection light. However, with solely the lens-shaped reflection layer is not good enough to improve the contrast ratio due to the scattering layer, which contributes a necessarily wide viewing angle, could interfere with both light paths before hitting the layer. So we propose a new design that sets the draft angle surface with absorption layer and adds an angle-selective absorber to separate these two kinds of light. The absorber is designed to fit the direction of the projection light, leading to a small absorption cross section for the projection light and respectfully big absorption cross section for the ambient light. We have calculated the design with Tracepro, a ray tracing program and find a nearly 8 times contrast ratio improvement against the current design in theory. This design can hopefully provide efficient display in bright lit situation with better viewer satisfaction.
Optical Polarization of Light from a Sorghum Canopy Measured Under Both a Clear and an Overcast Sky
NASA Technical Reports Server (NTRS)
Vanderbilt, Vern; Daughtry, Craig; Biehl, Larry; Dahlgren, Robert
2014-01-01
Introduction: We tested the hypothesis that the optical polarization of the light reflected by a sorghum canopy is due to a Fresnel-type redirection, by sorghum leaf surfaces, of light from an unpolarized light source, the sun or overcast sky, toward the measuring sensor. If it can be shown that the source of the polarization of the light scattered by the sorghum canopy is a first surface, Fresnel-type reflection, then removing this surface reflected light from measurements of canopy reflectance presumably would allow better insight into the biochemical processes such as photosynthesis and metabolism that occur in the interiors of sorghum canopy leaves. Methods: We constructed a tower 5.9m tall in the center of a homogenous sorghum field. We equipped two Barnes MMR radiometers with polarization analyzers on the number 1, 3 and 7 Landsat TM wavelength bands. Positioning the radiometers atop the tower, we collected radiance data in 44 view directions on two days, one day with an overcast sky and the other, clear and sunlit. From the radiance data we calculated the linear polarization of the reflected light for each radiometer wavelength channel and view direction. Results and Discussion: Our experimental results support our hypothesis, showing that the amplitude of the linearly polarized portion of the light reflected by the sorghum canopy varied dramatically with view azimuth direction under a point source, the sun, but the amplitude varied little with view azimuth direction under the hemispherical source, the overcast sky. Under the clear sky, the angle of polarization depended upon the angle of incidence of the sunlight on the leaf, while under the overcast sky the angle of polarization depended upon the zenith view angle. These results support a polarized radiation transport model of the canopy that is based upon a first surface, Fresnel reflection from leaves in the sorghum canopy.
Tsai, Yu-Lin; Liu, Che-Yu; Krishnan, Chirenjeevi; Lin, Da-Wei; Chu, You-Chen; Chen, Tzu-Pei; Shen, Tien-Lin; Kao, Tsung-Sheng; Charlton, Martin D B; Yu, Peichen; Lin, Chien-Chung; Kuo, Hao-Chung; He, Jr-Hau
2016-01-14
Green LEDs do not show the same level of performance as their blue and red cousins, greatly hindering the solid-state lighting development, which is the so-called "green gap". In this work, nano-void photonic crystals (NVPCs) were fabricated to embed within the GaN/InGaN green LEDs by using epitaxial lateral overgrowth (ELO) and nano-sphere lithography techniques. The NVPCs act as an efficient scattering back-reflector to outcouple the guided and downward photons, which not only boost the light extraction efficiency of LEDs with an enhancement of 78% but also collimate the view angle of LEDs from 131.5° to 114.0°. This could be because of the highly scattering nature of NVPCs which reduce the interference giving rise to Fabry-Perot resonance. Moreover, due to the threading dislocation suppression and strain relief by the NVPCs, the internal quantum efficiency was increased by 25% and droop behavior was reduced from 37.4% to 25.9%. The enhancement of light output power can be achieved as high as 151% at a driving current of 350 mA. Giant light output enhancement and directional control via NVPCs point the way towards a promising avenue of solid-state lighting.
NASA Astrophysics Data System (ADS)
Jo, Youngju; Jung, Jaehwang; Lee, Jee Woong; Shin, Della; Park, Hyunjoo; Nam, Ki Tae; Park, Ji-Ho; Park, Yongkeun
2014-05-01
Two-dimensional angle-resolved light scattering maps of individual rod-shaped bacteria are measured at the single-cell level. Using quantitative phase imaging and Fourier transform light scattering techniques, the light scattering patterns of individual bacteria in four rod-shaped species (Bacillus subtilis, Lactobacillus casei, Synechococcus elongatus, and Escherichia coli) are measured with unprecedented sensitivity in a broad angular range from -70° to 70°. The measured light scattering patterns are analyzed along the two principal axes of rod-shaped bacteria in order to systematically investigate the species-specific characteristics of anisotropic light scattering. In addition, the cellular dry mass of individual bacteria is calculated and used to demonstrate that the cell-to-cell variations in light scattering within bacterial species is related to the cellular dry mass and growth.
Yang, Defu; Chen, Xueli; Peng, Zhen; Wang, Xiaorui; Ripoll, Jorge; Wang, Jing; Liang, Jimin
2013-01-01
Modeling light propagation in the whole body is essential and necessary for optical imaging. However, non-scattering, low-scattering and high absorption regions commonly exist in biological tissues, which lead to inaccuracy of the existing light transport models. In this paper, a novel hybrid light transport model that couples the simplified spherical harmonics approximation (SPN) with the radiosity theory (HSRM) was presented, to accurately describe light transport in turbid media with non-scattering, low-scattering and high absorption heterogeneities. In the model, the radiosity theory was used to characterize the light transport in non-scattering regions and the SPN was employed to handle the scattering problems, including subsets of low-scattering and high absorption. A Neumann source constructed by the light transport in the non-scattering region and formed at the interface between the non-scattering and scattering regions was superposed into the original light source, to couple the SPN with the radiosity theory. The accuracy and effectiveness of the HSRM was first verified with both regular and digital mouse model based simulations and a physical phantom based experiment. The feasibility and applicability of the HSRM was then investigated by a broad range of optical properties. Lastly, the influence of depth of the light source on the model was also discussed. Primary results showed that the proposed model provided high performance for light transport in turbid media with non-scattering, low-scattering and high absorption heterogeneities. PMID:24156077
Yang, Defu; Chen, Xueli; Peng, Zhen; Wang, Xiaorui; Ripoll, Jorge; Wang, Jing; Liang, Jimin
2013-01-01
Modeling light propagation in the whole body is essential and necessary for optical imaging. However, non-scattering, low-scattering and high absorption regions commonly exist in biological tissues, which lead to inaccuracy of the existing light transport models. In this paper, a novel hybrid light transport model that couples the simplified spherical harmonics approximation (SPN) with the radiosity theory (HSRM) was presented, to accurately describe light transport in turbid media with non-scattering, low-scattering and high absorption heterogeneities. In the model, the radiosity theory was used to characterize the light transport in non-scattering regions and the SPN was employed to handle the scattering problems, including subsets of low-scattering and high absorption. A Neumann source constructed by the light transport in the non-scattering region and formed at the interface between the non-scattering and scattering regions was superposed into the original light source, to couple the SPN with the radiosity theory. The accuracy and effectiveness of the HSRM was first verified with both regular and digital mouse model based simulations and a physical phantom based experiment. The feasibility and applicability of the HSRM was then investigated by a broad range of optical properties. Lastly, the influence of depth of the light source on the model was also discussed. Primary results showed that the proposed model provided high performance for light transport in turbid media with non-scattering, low-scattering and high absorption heterogeneities.
Grigor Narekatsi's astronomical insights
NASA Astrophysics Data System (ADS)
Poghosyan, Samvel
2015-07-01
What stand out in the solid system of Gr. Narekatsi's naturalistic views are his astronomical insights on the material nature of light, its high speed and the Sun being composed of "material air". Especially surprising and fascinating are his views on stars and their clusters. What astronomers, including great Armenian academician V. Ambartsumian (scattering of stellar associations), would understand and prove with much difficulty thousand years later, Narekatsi predicted in the 10th century: "Stars appear and disappear untimely", "You who gather and scatter the speechless constellations, like a flock of sheep". Gr. Narekatsti's reformative views were manifested in all the spheres of the 10th century social life; he is a reformer of church life, great language constructor, innovator in literature and music, freethinker in philosophy and science. His ideology is the reflection of the 10th century Armenian Renaissance. During the 9th-10th centuries, great masses of Armenians, forced to migrate to the Balkans, took with them and spread reformative ideas. The forefather of the western science, which originated in the period of Reformation, is considered to be the great philosopher Nicholas of Cusa. The study of Gr. Narekatsti's logic and naturalistic views enables us to claim that Gr. Narekatsti is the great grandfather of European science.
View From a Megacity: Aerosol Light Absorption and Scattering at Four Sites in and Near Mexico City.
NASA Astrophysics Data System (ADS)
Paredes-Miranda, G.; Arnott, W. P.; Gaffney, J. S.; Marley, N. A.
2006-12-01
As part of the Megacity Impacts on Regional and Global Environments, MIRAGE-Mex deployment to Mexico City in the period of 30 days, March 2006, a suite of photoacoustic spectrometers (PAS) were installed to measure at ground level the light absorption and scattering by aerosols at four sites: an urban site at Instituto Mexicano del Petroleo (Mexican Oil Institute, denoted by IMP), a suburban site at the Technological University of Tecamac, a rural site at "La Biznaga" ranch, and a site at the Paseo de Cortes (altitude 3,810 meters ASL) in the rural area above Amecameca in the State of Mexico, on the saddle between the volcanoes Popocatepetl and Iztaccihuatl. The IMP site gave in-situ characterization of the Mexico City plume under favorable wind conditions while the other sites provided characterization of the plume, mixed in with any local sources. The second and third sites are north of Mexico City, and the fourth site is south. The PAS used at IMP operates at 532 nm, and conveniently allowed for characterization of gaseous absorption at this wavelength as well. Instruments at the second and third sites operate at 870 nm, and the one at the fourth site at 780 nm. Light scattering measurements are accomplished within the PAS by the reciprocal nephelometery method. In the urban site the aerosol absorption coefficient typically varies between 40 and 250 Mm-1 during the course of the day and significant diurnal variation of the aerosol single scattering albedo was observed. Comparisons with TSI nephelometer scattering and Aetholemeter absorption measurements at the T0 site will be presented. We will present a broad overview of the diurnal variation of the scattering and absorption as well as the single scattering albedo and fraction of absorption due to gases at the IMP site. Insight on the dynamical connections will be discussed.
Biological instrumentation for the Viking 1975 mission to Mars.
Klein, H P; Vishniac, W
1972-01-01
A brief introduction is given on why Mars is of interest from a biological point of view, along with an overview of the Viking 1975 mission. Details are given about the four biology instruments aboard the spacecraft and the experiments for which they are to be used. These are: the carbon assimilation experiment to determine whether the soil is biologically active, by incubation in presence of 14C-labelled CO and CO2 (known to be present in the Martian atmosphere); the label release experiment to detect metabolic activity by the release of radioactive CO2, from 14C-labelled simple organic substrates; the gas exchange experiment to detect biological activity by repeated gas chromatography analysis of soil samples; the light scattering experiment, where increase of scattering and decrease of light transmission would indicate the growth of organisms. Examples are given of data obtained with terrestrial soils in these experiments.
NASA Technical Reports Server (NTRS)
1997-01-01
Based on a Small Business Innovation Research contract from the Jet Propulsion Laboratory, TracePro is state-of-the-art interactive software created by Lambda Research Corporation to detect stray light in optical systems. An image can be ruined by incidental light in an optical system. To maintain image excellence from an optical system, stray light must be detected and eliminated. TracePro accounts for absorption, specular reflection and refraction, scattering and aperture diffraction of light. Output from the software consists of spatial irradiance plots and angular radiance plots. Results can be viewed as contour maps or as ray histories in tabular form. TracePro is adept at modeling solids such as lenses, baffles, light pipes, integrating spheres, non-imaging concentrators, and complete illumination systems. The firm's customer base includes Lockheed Martin, Samsung Electronics and other manufacturing, optical, aerospace, and educational companies worldwide.
Non-label bioimaging utilizing scattering lights
NASA Astrophysics Data System (ADS)
Watanabe, Tomonobu M.; Ichimura, Taro; Fujita, Hideaki
2017-04-01
Optical microscopy is an indispensable tool for medical and life sciences. Especially, the microscopes utilized with scattering light offer a detailed internal observation of living specimens in real time because of their non-labeling and non-invasive capability. We here focus on two kinds of scattering lights, Raman scattering light and second harmonic generation light. Raman scattering light includes the information of all the molecular vibration modes of the molecules, and can be used to distinguish types and/or state of cell. Second harmonic generation light is derived from electric polarity of proteins in the specimen, and enables to detect their structural change. In this conference, we would like to introduce our challenges to extract biological information from those scattering lights.
Organic electroluminescent devices having improved light extraction
Shiang, Joseph John [Niskayuna, NY
2007-07-17
Organic electroluminescent devices having improved light extraction include a light-scattering medium disposed adjacent thereto. The light-scattering medium has a light scattering anisotropy parameter g in the range from greater than zero to about 0.99, and a scatterance parameter S less than about 0.22 or greater than about 3.
NASA Astrophysics Data System (ADS)
Merino, Enrique; Kozub, Tom; Boyle, Dennis; Lucia, Matthew; Majeski, Richard; Kaita, Robert; Schmitt, John C.; Leblanc, Benoit; Diallo, Ahmed; Jacobson, C. M.
2014-10-01
The Thomson Scattering (TS) System in LTX is used to measure electron temperature and density profiles of core and edge plasmas. In view of TS measurements showing low signal-to-noise and high stray light, numerous improvements were performed in recent months. These will allow for better measurements. Due to the nature of LTX's lithium coated walls, a particular challenge was presented by alignment procedures which required insertion and precise positioning of equipment in the vacuum vessel without breaking vacuum. To overcome these difficulties, the laser flight tubes were removed and an alignment probe setup placed along the beam line on a differentially pumped assembly. The probe was then driven into the vacuum vessel and back-illumination of the viewing optics on it allowed for alignment and spatial calibration. Other upgrades included better bracing of flight tubes and viewing optics as well as a redesigned beam dump. An overview of these improvements will be presented. Supported by US DOE Contracts DE-AC02-09CH11466 and DE-AC52-07NA27344.
2016-09-05
Saturn's rings appear to bend as they pass behind the planet's darkened limb due to refraction by Saturn's upper atmosphere. The effect is the same as that seen in an earlier Cassini view (see PIA20491), except this view looks toward the unlit face of the rings, while the earlier image viewed the rings' sunlit side. The difference in illumination brings out some noticeable differences. The A ring is much darker here, on the rings' unlit face, since its larger particles primarily reflect light back toward the sun (and away from Cassini's cameras in this view). The narrow F ring (at bottom), which was faint in the earlier image, appears brighter than all of the other rings here, thanks to the microscopic dust that is prevalent within that ring. Small dust tends to scatter light forward (meaning close to its original direction of travel), making it appear bright when backlit. (A similar effect has plagued many a driver with a dusty windshield when driving toward the sun.) This view looks toward the unilluminated side of the rings from about 19 degrees below the ring plane. The image was taken in red light with the Cassini spacecraft narrow-angle camera on July 24, 2016. The view was acquired at a distance of approximately 527,000 miles (848,000 kilometers) from Saturn and at a sun-Saturn-spacecraft, or phase, angle of 169 degrees. Image scale is 3 miles (5 kilometers) per pixel. http://photojournal.jpl.nasa.gov/catalog/PIA20497
Power, J F
2009-06-01
Light profile microscopy (LPM) is a direct method for the spectral depth imaging of thin film cross-sections on the micrometer scale. LPM uses a perpendicular viewing configuration that directly images a source beam propagated through a thin film. Images are formed in dark field contrast, which is highly sensitive to subtle interfacial structures that are invisible to reference methods. The independent focusing of illumination and imaging systems allows multiple registered optical sources to be hosted on a single platform. These features make LPM a powerful multi-contrast (MC) imaging technique, demonstrated in this work with six modes of imaging in a single instrument, based on (1) broad-band elastic scatter; (2) laser excited wideband luminescence; (3) coherent elastic scatter; (4) Raman scatter (three channels with RGB illumination); (5) wavelength resolved luminescence; and (6) spectral broadband scatter, resolved in immediate succession. MC-LPM integrates Raman images with a wider optical and morphological picture of the sample than prior art microprobes. Currently, MC-LPM resolves images at an effective spectral resolution better than 9 cm(-1), at a spatial resolution approaching 1 microm, with optics that operate in air at half the maximum numerical aperture of the prior art microprobes.
Catheter guided by optical coherence domain reflectometry
Everett, Matthew; Colston, Billy W.; Da Silva, Luiz B.; Matthews, Dennis
2002-01-01
A guidance and viewing system based on multiplexed optical coherence domain reflectometry is incorporated into a catheter, endoscope, or other medical device to measure the location, thickness, and structure of the arterial walls or other intra-cavity regions at discrete points on the medical device during minimally invasive medical procedures. The information will be used both to guide the device through the body and to evaluate the tissue through which the device is being passed. Multiple optical fibers are situated along the circumference of the device. Light from the distal end of each fiber is directed onto the interior cavity walls via small diameter optics (such as gradient index lenses and mirrored corner cubes). Both forward viewing and side viewing fibers can be included. The light reflected or scattered from the cavity walls is then collected by the fibers and multiplexed at the proximal end to the sample arm of an optical low coherence reflectometer. The system may also be implemented in a nonmedical inspection device.
Deep HST/STIS Visible-Light Imaging of Debris Systems Around Solar Analog Hosts
NASA Technical Reports Server (NTRS)
Schneider, Glenn; Grady, Carol A.; Stark, Christopher C.; Gaspar, Andras; Carson, Joseph; Debes, John H.; Henning, Thomas; Hines, Dean C.; Jang-Condell, Hannah; Kuchner, Marc J.
2016-01-01
We present new Hubble Space Telescope observations of three a priori known starlight-scattering circumstellar debris systems (CDSs) viewed at intermediate inclinations around nearby close-solar analog stars: HD 207129, HD202628, and HD 202917. Each of these CDSs possesses ring-like components that are more massive analogs of our solar systems Edgeworth Kuiper Belt. These systems were chosen for follow-up observations to provide imaging with higher fidelity and better sensitivity for the sparse sample of solar-analog CDSs that range over two decades in systemic ages, with HD 202628 and HD 207129 (both approx. 2.3 Gyr) currently the oldest CDSs imaged in visible or near-IR light. These deep (10-14 ks) observations, made with six-roll point-spread-function template visible-light coronagraphy using the Space Telescope Imaging Spectrograph, were designed to better reveal their angularly large debris rings of diffuse low surface brightness, and for all targets probe their exo-ring environments for starlight-scattering materials that present observational challenges for current ground-based facilities and instruments. Contemporaneously also observing with a narrower occulter position, these observations additionally probe the CDS endo-ring environments that are seen to be relatively devoid of scatterers. We discuss the morphological, geometrical, and photometric properties of these CDSs also in the context of other CDSs hosted by FGK stars that we have previously imaged as a homogeneously observed ensemble. From this combined sample we report a general decay in quiescent-disk F disk /F star optical brightness approx. t( exp.-0.8), similar to what is seen at thermal IR wavelengths, and CDSs with a significant diversity in scattering phase asymmetries, and spatial distributions of their starlight-scattering grains.
Apparatus for measuring particle properties
Rader, Daniel J.; Castaneda, Jaime N.; Grasser, Thomas W.; Brockmann, John E.
1998-01-01
An apparatus for determining particle properties from detected light scattered by the particles. The apparatus uses a light beam with novel intensity characteristics to discriminate between particles that pass through the beam and those that pass through an edge of the beam. The apparatus can also discriminate between light scattered by one particle and light scattered by multiple particles. The particle's size can be determined from the intensity of the light scattered. The particle's velocity can be determined from the elapsed time between various intensities of the light scattered.
2000-01-20
In this lunar eclipse viewed from Merritt Island, Fla., at 11:55 p.m., the full moon takes on a dark red color because it is being lighted slightly by sunlight passing through the Earth's atmosphere. This light has the blue component preferentially scattered out (this is also why the sky appears blue from the surface of the Earth), leaving faint reddish light to illuminate the Moon during the eclipse. Eclipses occur when the Sun, Earth and Moon line up. They are rare because the Moon usually passes above or below the imaginary line connecting Earth and the Sun. The Earth casts a shadow that the Moon can pass through -when it does, it is called a lunar eclipse
2000-01-20
In this lunar eclipse viewed from Merritt Island, Fla., at midnight, the full moon takes on a dark red color because it is being lighted slightly by sunlight passing through the Earth's atmosphere. This light has the blue component preferentially scattered out (this is also why the sky appears blue from the surface of the Earth), leaving faint reddish light to illuminate the Moon during the eclipse. Eclipses occur when the Sun, Earth and Moon line up. They are rare because the Moon usually passes above or below the imaginary line connecting Earth and the Sun. The Earth casts a shadow that the Moon can pass through -when it does, it is called a lunar eclipse
Optical coherence domain reflectometry guidewire
Colston, Billy W.; Everett, Matthew; Da Silva, Luiz B.; Matthews, Dennis
2001-01-01
A guidewire with optical sensing capabilities is based on a multiplexed optical coherence domain reflectometer (OCDR), which allows it to sense location, thickness, and structure of the arterial walls or other intra-cavity regions as it travels through the body during minimally invasive medical procedures. This information will be used both to direct the guidewire through the body by detecting vascular junctions and to evaluate the nearby tissue. The guidewire contains multiple optical fibers which couple light from the proximal to distal end. Light from the fibers at the distal end of the guidewire is directed onto interior cavity walls via small diameter optics such as gradient index lenses and mirrored corner cubes. Both forward viewing and side viewing fibers can be included. The light reflected or scattered from the cavity walls is then collected by the fibers, which are multiplexed at the proximal end to the sample arm of an optical low coherence reflectometer. The guidewire can also be used in nonmedical applications.
Backscatter measurements for NIF ignition targets (invited).
Moody, J D; Datte, P; Krauter, K; Bond, E; Michel, P A; Glenzer, S H; Divol, L; Niemann, C; Suter, L; Meezan, N; MacGowan, B J; Hibbard, R; London, R; Kilkenny, J; Wallace, R; Kline, J L; Knittel, K; Frieders, G; Golick, B; Ross, G; Widmann, K; Jackson, J; Vernon, S; Clancy, T
2010-10-01
Backscattered light via laser-plasma instabilities has been measured in early NIF hohlraum experiments on two beam quads using a suite of detectors. A full aperture backscatter system and near backscatter imager (NBI) instrument separately measure the stimulated Brillouin and stimulated Raman scattered light. Both instruments work in conjunction to determine the total backscattered power to an accuracy of ∼15%. In order to achieve the power accuracy we have added time-resolution to the NBI for the first time. This capability provides a temporally resolved spatial image of the backscatter which can be viewed as a movie.
2004-09-23
Looking beyond Saturn's south pole, this was the Cassini spacecraft's view of the distant, icy moon Enceladus on July 28, 2004. The planet itself shows few obvious features at these ultraviolet wavelengths, due to scattering of light by molecules of the gases high in the atmosphere. Enceladus is 499 kilometers (310 miles) wide. The image was taken with the Cassini spacecraft narrow angle camera at a distance of 7.4 million kilometers (4.6 million miles) from Saturn through a filter sensitive to ultraviolet wavelengths of light. The image scale is 44 kilometers (27 miles) per pixel of Saturn. http://photojournal.jpl.nasa.gov/catalog/PIA06483
Scatter Measurements Made With Ultraviolet Light
NASA Astrophysics Data System (ADS)
Anthon, Erik W.
1985-09-01
The quality of optical surfaces is generally evaluated by how much light (normally visible light) is scattered by the surface. Most optical glasses and many coating materials are completely opaque to ultraviolet light (253.7 nm). Ultraviolet light tends to scatter much more than visible light. Scatter measurements made with ultraviolet light are therefore very sensitive and the scatter from second surfaces and from the interior (bulk) of the optical material is eliminated by the opacity. A novel scattermeter that operates with ultraviolet light has been developed. The construction and operation of this scattermeter will be described. Cleaning soon becomes the limiting factor when measuring the surfaces with very low level of scatter. Sensitivity to repeated cleaning has been investigated. Different surfaces are compared and uniformity of surfaces is measured by mapping a surface area with an x-y stage. Polished glass surfaces generally have much higher scatter than natural glass surfaces (fire polished, drawn or floated surfaces). Very low scatter levels have been found on thin drawn glass.
NASA Astrophysics Data System (ADS)
Tseng, Snow H.; Chang, Shih-Hui
2018-04-01
Here we present a numerical simulation to analyze the effect of scattering on focusing light into closely-spaced twin peaks. The pseudospectral time-domain (PSTD) is implemented to model continuous-wave (CW) light propagation through a scattering medium. Simulations show that CW light can propagate through a scattering medium and focus into closely-spaced twin peaks. CW light of various wavelengths focusing into twin peaks with sub-diffraction spacing is simulated. In advance, light propagation through scattering media of various number densities is simulated to decipher the dependence of CW light focusing phenomenon on the scattering medium. The reported simulations demonstrate the feasibility of focusing CW light into twin peaks with sub-diffraction dimensions. More importantly, based upon numerical solutions of Maxwell’s equations, research findings show that the sub-diffraction focusing phenomenon can be achieved with scarce or densely-packed scattering media.
Monodisperse ferrous phosphate colloids in an anoxic groundwater plume
Gschwend, Philip M.; Reynolds, Matthew D.
1987-01-01
Groundwater samples collected near a secondary-sewage infiltration site on Cape Cod, Massachusetts were examined for colloidal materials (10–1000 nm). In two wells the water contained a population of monodisperse 100-nm particles, detected using laser-light scattering and autocorrelation data processing. SEM and SEM-EDAX analysis of these colloidal materials collected on ultrafilters confirmed the laser light scattering result and revealed that these microparticles consisyed of primarily iron and phosphorus in a 1.86 Fe to 1.0 P stoichiometric ratio. Chemical analyses of the water samples, together with equilibrium solubility calculations, strongly suggest that the ion-activity product should exceed the solubility product of a 100-nm diameter predominantly vivianite-type (Fe3(PO4)2 · 8H2O) colloidal phase. In light of our results, we conclude that these microparticles were formed by sewage-derived phosphate combining with ferrous iron released from the aquifer solids, and that these colloids may be moving in the groundwater flow. Such a subsurface transport process could have major implications regarding the movement of particle-reactive pollutants traditionally viewed as non-mobile in groundwater.
Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells
NASA Astrophysics Data System (ADS)
Park, Yongkeun; Diez-Silva, Monica; Fu, Dan; Popescu, Gabriel; Choi, Wonshik; Barman, Ishan; Suresh, Subra; Feld, Michael S.
2010-03-01
We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to the scattering signal arising from the development of malaria-inducing parasites. By selectively imaging the electric fields using quantitative phase microscopy and a Fourier transform light scattering technique, we calculate the light scattering maps of individual Pf-RBCs. We show that the onset and progression of pathological states of the Pf-RBCs can be clearly identified by the static scattering maps. Progressive changes to the biophysical properties of the Pf-RBC membrane are captured from dynamic light scattering.
Apparatus for measuring particle properties
Rader, D.J.; Castaneda, J.N.; Grasser, T.W.; Brockmann, J.E.
1998-08-11
An apparatus is described for determining particle properties from detected light scattered by the particles. The apparatus uses a light beam with novel intensity characteristics to discriminate between particles that pass through the beam and those that pass through an edge of the beam. The apparatus can also discriminate between light scattered by one particle and light scattered by multiple particles. The particle`s size can be determined from the intensity of the light scattered. The particle`s velocity can be determined from the elapsed time between various intensities of the light scattered. 11 figs.
Minami, Keiichiro; Maruyama, Yoko; Mihashi, Toshifumi; Miyata, Kazunori; Oshika, Tetsuro
2017-03-01
To evaluate the influence of increases in light scattering on intraocular lens (IOL) surfaces on paraxial forward scattering using goniophotometry and Hartmann-Shack wavefront aberrometry. Surface light scattering was reproduced experimentally by acceleratedly aging 4 intraocular lenses by 0, 3, 5, and 10 years each. Light scattering from both IOL surfaces was measured using Scheimpflug photography. The paraxial forward scattering from the aged IOLs was measured using a goniophotometer with a halogen light source (wavelength: 350-850 nm) and telecentric optics, and changes in the maximum intensity and full width at 10% of maximum intensity (FW10%) were evaluated. The influences on the retina image were examined using a Hartmann-Shack aberrometer (wavelength: 840 nm). The contrast and difference from the point spread function of the central centroids were evaluated. The mean surface light scattering from both IOL surfaces ranged from 30.0 to 118.3 computer compatible tape (CCT) and increased with each aging year. Evaluations using the goniophotometer and the Hartmann-Shack aberrometer showed no significant change in the paraxial forward scattering with the aging year (P > .45, Kruskal-Wallis test), and no association with the surface light scattering intensity was found (P > .75, Spearman rank correlation). This experimental study using aged IOLs demonstrated that surface light scattering does not influence paraxial forward scattering.
Laser Light Scattering with Multiple Scattering Suppression Used to Measure Particle Sizes
NASA Technical Reports Server (NTRS)
Meyer, William V.; Tin, Padetha; Lock, James A.; Cannell, David S.; Smart, Anthony E.; Taylor, Thomas W.
1999-01-01
Laser light scattering is the technique of choice for noninvasively sizing particles in a fluid. The members of the Advanced Technology Development (ATD) project in laser light scattering at the NASA Lewis Research Center have invented, tested, and recently enhanced a simple and elegant way to extend the concentration range of this standard laboratory particle-sizing technique by several orders of magnitude. With this technique, particles from 3 nm to 3 mm can be measured in a solution. Recently, laser light scattering evolved to successfully size particles in both clear solutions and concentrated milky-white solutions. The enhanced technique uses the property of light that causes it to form tall interference patterns at right angles to the scattering plane (perpendicular to the laser beam) when it is scattered from a narrow laser beam. Such multiple-scattered light forms a broad fuzzy halo around the focused beam, which, in turn, forms short interference patterns. By placing two fiber optics on top of each other and perpendicular to the laser beam (see the drawing), and then cross-correlating the signals they produce, only the tall interference patterns formed by singly scattered light are detected. To restate this, unless the two fiber optics see the same interference pattern, the scattered light is not incorporated into the signal. With this technique, only singly scattered light is seen (multiple-scattered light is rejected) because only singly scattered light has an interference pattern tall enough to span both of the fiber-optic pickups. This technique is simple to use, easy to align, and works at any angle. Placing a vertical slit in front of the signal collection fibers enhanced this approach. The slit serves as an optical mask, and it significantly shortens the time needed to collect good data by selectively masking out much of the unwanted light before cross-correlation is applied.
Roy, Gilles; Roy, Nathalie
2008-03-20
A multiple-field-of-view (MFOV) lidar is used to characterize size and optical depth of low concentration of bioaerosol clouds. The concept relies on the measurement of the forward scattered light by using the background aerosols at various distances at the back of a subvisible cloud. It also relies on the subtraction of the background aerosol forward scattering contribution and on the partial attenuation of the first-order backscattering. The validity of the concept developed to retrieve the effective diameter and the optical depth of low concentration bioaerosol clouds with good precision is demonstrated using simulation results and experimental MFOV lidar measurements. Calculations are also done to show that the method presented can be extended to small optical depth cloud retrieval.
Suppression of sun interference in the star sensor baffling stray light by total internal reflection
NASA Astrophysics Data System (ADS)
Kawano, Hiroyuki; Shimoji, Haruhiko; Yoshikawa, Shoji; Miyatake, Katsumasa; Hama, Kazumori; Nakamura, Shuji
2005-09-01
We have developed a star sensor as an experimental device onboard the SERVIS-1 satellite launched in October 2003. The in-orbit data have verified its fundamental performance. One of the advantages of our star sensor is that the baffle has a small length of 120 mm instead of 182 mm in the conventional two-stage baffle design. The key concepts for light shielding are total internal reflection phenomena inside a nearly half sphere (NHS) lens and scattering light control by gloss black paint. However, undesirable background noise by the sun outside of the field of view (FOV) was observed in the corner of the FOV in the orbital experiment. Ray trace simulations revealed that slight scattering light on the specular baffle wall entered the NHS lens and reached the corner of the image sensor through the multi-reflection path inside the lens. It was found that the stray light path can be shielded effectively if the diameter of the aperture under the NHS lens was reduced. We redesigned the baffle and evaluated the light shielding ability with our sun interference test facility on the ground, and confirmed that the stray light was reduced below the acceptable level. As a result, the light shielding technique which we have proposed was proved to be effective for a small-size baffle. The redesigned star sensor is planned to be installed as a main attitude sensor for the SERVIS-2 satellite scheduled to be launched in February 2008.
Bacterial Identification Using Light Scattering Measurements: a Preliminary Report
NASA Technical Reports Server (NTRS)
Wilkins, J. R.
1971-01-01
The light scattering properties of single bacterial cells were examined as a possible means of identification. Three species were studied with streptococcus faecalis exhibiting a unique pattern; the light-scattering traces for staphylococcus aureus and escherichia coli were quite similar although differences existed. Based on preliminary investigations, the light scattering approach appeared promising with additional research needed to include a wide variety of bacterial species, computer capability to handle and analyze data, and expansion of light scattering theory to include bacterial cells.
NASA Astrophysics Data System (ADS)
Stradner, Anna; Bucciarelli, Saskia; Casal, Lucia; Foffi, Giuseppe; Thurston, George; Farago, Bela; Schurtenberger, Peter
2014-03-01
The occurrence of an arrest transition in concentrated colloid suspensions and its dependence on the interaction potential is a hot topic in soft matter. Such arrest transitions can also occur in concentrated protein solutions, as they exist e.g. in biological cells or are increasingly used in pharmaceutical formulations. Here we demonstrate the applicability of concepts from colloid science to understand the dynamics of concentrated protein solutions. In this presentation we report a combination of 3D light scattering, small-angle X-ray scattering and neutron spin echo measurements to study the structural properties as well as the collective and self diffusion of proteins in highly concentrated solutions on the relevant length and time scales. We demonstrate that various arrest scenarios indeed exist for different globular proteins. The proteins chosen are different bovine lens crystallins. We report examples of hard and attractive glass transitions and arrested spinodal decomposition directly linked to the effective pair potentials determined in static scattering experiments for the different proteins. We discuss these different arrest scenarios in view of possible applications of dense protein solutions as well as in view of their possible relevance for living systems.
Scattering theory of stochastic electromagnetic light waves.
Wang, Tao; Zhao, Daomu
2010-07-15
We generalize scattering theory to stochastic electromagnetic light waves. It is shown that when a stochastic electromagnetic light wave is scattered from a medium, the properties of the scattered field can be characterized by a 3 x 3 cross-spectral density matrix. An example of scattering of a spatially coherent electromagnetic light wave from a deterministic medium is discussed. Some interesting phenomena emerge, including the changes of the spectral degree of coherence and of the spectral degree of polarization of the scattered field.
Anisotropic light scattering of individual sickle red blood cells.
Kim, Youngchan; Higgins, John M; Dasari, Ramachandra R; Suresh, Subra; Park, YongKeun
2012-04-01
We present the anisotropic light scattering of individual red blood cells (RBCs) from a patient with sickle cell disease (SCD). To measure light scattering spectra along two independent axes of elongated-shaped sickle RBCs with arbitrary orientation, we introduce the anisotropic Fourier transform light scattering (aFTLS) technique and measured both the static and dynamic anisotropic light scattering. We observed strong anisotropy in light scattering patterns of elongated-shaped sickle RBCs along its major axes using static aFTLS. Dynamic aFTLS analysis reveals the significantly altered biophysical properties in individual sickle RBCs. These results provide evidence that effective viscosity and elasticity of sickle RBCs are significantly different from those of the healthy RBCs.
Progress on Thomson scattering in the Pegasus Toroidal Experiment
NASA Astrophysics Data System (ADS)
Schlossberg, D. J.; Bongard, M. W.; Fonck, R. J.; Schoenbeck, N. L.; Winz, G. R.
2013-11-01
A novel Thomson scattering system has been implemented on the Pegasus Toroidal Experiment where typical densities of 1019 m-3 and electron temperatures of 10 to 500 eV are expected. The system leverages technological advances in high-energy pulsed lasers, volume phase holographic (VPH) diffraction gratings, and gated image intensified (ICCD) cameras to provide a relatively low-maintenance, economical, robust diagnostic system. Scattering is induced by a frequency-doubled, Q-switched Nd:YAG laser (2 J at 532 nm, 7 ns FWHM pulse) directed to the plasma over a 7.7 m long beam path, and focused to < 3 mm throughout the collection region. Inter-shot beam alignment is adjustable with less than a 0.01 mm spatial resolution in the collection region. A custom lens system collects scattered photons at radii 15 cm to 85 cm from the machine's center, at ~ F/6 with 14 mm radial resolution. The initial configuration provides scattering measurements at 12 spatial locations and 12 simultaneous background measurements at adjacent locations. If plasma background subtraction proves to be insignificant, these background channels will be used as viewing channels. Each spectrometer supports 8 spatial channels and can provide 8 or more spectral bins each. The spectrometers use high-efficiency VPH transmission gratings (eff. > 80%) and fast-gated ICCDs (gate > 2 ns, Gen III intensifier) with high-throughput (F/1.8), achromatic lensing. A stray light mitigation facility has been implemented, consisting of a multi-aperture optical baffle system and a simple beam dump. Successful stray light reduction has enabled detection of scattered signal, and Rayleigh scattering has been used to provide a relative calibration. Initial temperature measurements have been made and data analysis algorithms are under development.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Alekseev, A E; Potapov, V T; Gorshkov, B G
We report the results of studying statistical properties of the intensity of partially polarised coherent light backscattered by a single mode optical fibre. An expression is derived for the deviation of the backscattered light intensity depending on the scattering region length, the degree of the light source coherence and the degree of scattered light polarisation. It is shown that the backscattered light in a fibre scattered-light interferometer is partially polarised with the polarisation degree P = 1/3 in the case of external perturbations of the interferometer fibre. (scattering of light)
A study of the polarization of light scattered by vegetation. M.S. Thesis
NASA Technical Reports Server (NTRS)
Woessner, P. N.
1985-01-01
This study was undertaken in order to better understand the factors that govern the polarization of light scattered from vegetation and soils. The intensity and polarization of light scattered by clover and grass in vivo and soil were measured at a number of different angles of incidence and reflectance. Both individual leaves and natural patches of leaves were measured. The light transmitted through the leaves was found to be negatively polarized. The light scattered from the upper leaf surface was found to be positively polarized in a manner which could be accounted for qualitatively but not quantitatively by the Fresnel reflection coefficients modified by a shadowing function of the form cos sup2 (g/2), where g is the phase angle. Findings indicate that the polarization of light scattered by vegetation is a more complex process than previously thought, and that besides the surface-scattered component of light, the volume-scattered and multiply-scattered components also contribute significantly to the polarization.
NASA Astrophysics Data System (ADS)
Turcu, Ioan; Bratfalean, Radu; Neamtu, Silvia
2008-07-01
The adequacy of the effective phase function (EPF) used to describe the light scattered at small angles was tested on aqueous suspensions of polystyrene microspheres. Angular resolved light scattering measurements were performed on two types of latex suspension, which contained polystyrene spheres of 3 µm and 5 µm diameters, respectively. The experimental data were fitted with two EPF approximants. If the polystyrene spheres are at least 3 µm in diameter the quasi-ballistic light scattering process can be described relatively well by the EPF in a small angular range centered in the forward direction. The forward light scattering by macroscopic samples containing microspheres can be modeled relatively well if the true Mie single particle scattering phase function is replaced by a simpler Henyey-Greenstein dependence having the same width at half-height as the first scattering lobe.
NASA Astrophysics Data System (ADS)
Zhou, Hongwei; Xu, Shenghua; Mi, Li; Sun, Zhiwei; Qin, Yanming
2014-09-01
Absolute coagulation rate constants were determined by independently, instead of simultaneously, using static and dynamic light scattering with the requested optical factors calculated by T-matrix method. The aggregating suspensions of latex particles with diameters of 500, 700, and 900 nm, that are all beyond validity limit of the traditional Rayleigh-Debye-Gans approximation, were adopted. The results from independent static and dynamic light scattering measurements were compared with those by simultaneously using static and dynamic light scattering; and three of them show good consistency. We found, theoretically and experimentally, that for independent static light scattering measurements there are blind scattering angles at that the scattering measurements become impossible and the number of blind angles increases rapidly with particle size. For independent dynamic light scattering measurements, however, there is no such a blind angle at all. A possible explanation of the observed phenomena is also presented.
Instrumentation on Multi-Scaled Scattering of Bio-Macromolecular Solutions
Chu, Benjamin; Fang, Dufei; Mao, Yimin
2015-01-01
The design, construction and initial tests on a combined laser light scattering and synchrotron X-ray scattering instrument can cover studies of length scales from atomic sizes in Angstroms to microns and dynamics from microseconds to seconds are presented. In addition to static light scattering (SLS), dynamic light scattering (DLS), small angle X-ray scattering (SAXS) and wide angle X-ray diffraction (WAXD), the light scattering instrument is being developed to carry out studies in mildly turbid solutions, in the presence of multiple scattering. Three-dimensional photon cross correlation function (3D-PCCF) measurements have been introduced to couple with synchrotron X-ray scattering to study the structure, size and dynamics of macromolecules in solution. PMID:25946340
HUBBLE'S DEEPEST VIEW OF THE UNIVERSE
NASA Technical Reports Server (NTRS)
2002-01-01
[Left] A NASA Hubble Space Telescope view of the faintest galaxies ever seen in the universe, taken in infrared light with the Near Infrared Camera and Multi-Object Spectrometer (NICMOS). The picture contains over 300 galaxies having spiral, elliptical and irregular shapes. Though most of these galaxies were first seen in 1995 when Hubble was used to take a visible-light deep exposure of the same field, NICMOS uncovers many new objects. Most of these objects are too small and faint to be apparent in the full field NICMOS view. Some of the reddest and faintest of the newly detected objects may be over 12 billion light-years away, as derived from a standard model of the universe. However, a powerful new generation of telescopes will be needed to confirm the suspected distances of these objects. The field of view is 2 million light-years across, at its maximum. Yet, on a cosmic scale, it represents only a thin pencil beam look across the universe. The area of sky is merely 1/100th the apparent diameter on the full moon. [Right] Two close-up NICMOS views of candidate objects which may be over 12 billion light-years away. Each candidate is centered in the frame. The reddish color may mean all of the starlight has been stretched to infrared wavelengths by the universe's expansion. Alternative explanations are that the objects are closer to us, but the light has been reddened by dust scattering. A new generation of telescopes will be needed to make follow-up observations capable of establishing true distance. The image was taken in January 1998 and required an exposure time of 36 hours to detect objects down to 30th magnitude. Hubble was aimed in the direction of the constellation Ursa Major, in a region just above the handle of the Big Dipper. The color corresponds to blue (0.45 microns), green (1.1 microns) and red (1.6 microns). Credit: Rodger I. Thompson (University of Arizona), and NASA
Multi-peaks scattering of light in glasses
NASA Astrophysics Data System (ADS)
Smirnov, V. A.; Vostrikova, L. I.
2018-04-01
Investigations of the multi-peaks scattering of the laser light on the micro-scale susceptibility gratings with small periodicities photo-induced in the various glass materials are presented. The observed pictures of the multi-peaks scattering of light in oxide samples show that the efficiencies of the processes of scattering can vary for the different chemical compositions. Experimental results are in agreement with the proposed theory of light scattering.
Diffusing Wave Spectroscopy Used to Study Foams
NASA Technical Reports Server (NTRS)
Zimmerli, Gregory A.; Durian, Douglas J.
2000-01-01
The white appearance of familiar objects such as clouds, snow, milk, or foam is due to the random scattering of light by the sample. As we all know, pure water is clear and easily passes a beam of light. However, tiny water droplets, such as those in a cloud, scatter light because the air and water droplet have different indexes of refraction. When many droplets, or scattering sites, are present, the incident light is scattered in random directions and the sample takes on a milky white appearance. In a glass of milk, the scattering is due to small colloidal particles. The white appearance of shaving cream, or foam, is due to the scattering of light at the water-bubble interface. Diffusing wave spectroscopy (DWS) is a laser light-scattering technique used to noninvasively probe the particle dynamics in systems that strongly scatter light. The technique takes advantage of the diffuse nature of light, which is reflected or transmitted from samples such as foams, dense colloidal suspensions (such as paint and milk), emulsions, liquid crystals, sandpiles, and even biological tissues.
Sources of background light on space based laser communications links
NASA Astrophysics Data System (ADS)
Farrell, Thomas C.
2018-05-01
We discuss the sources and levels of background light that should be expected on space based laser communication (lasercom) crosslinks and uplinks, as well as on downlinks to ground stations. The analyses are valid for both Earth orbiting satellites and inter-planetary links. Fundamental equations are derived suitable for first order system engineering analyses of potential lasercom systems. These divide sources of background light into two general categories: extended sources which fill the field of view of a receiver's optics, and point sources which cannot be resolved by the optics. Specific sources of background light are discussed, and expected power levels are estimated. For uplinks, reflected sunlight and blackbody radiation from the Earth dominates. For crosslinks, depending on specific link geometry, sources of background light may include the Sun in the field of view (FOV), reflected sunlight and blackbody radiation from planets and other bodies in the solar system, individual bright stars in the FOV, the amalgam of dim stars in the FOV, zodiacal light, and reflected sunlight off of the transmitting spacecraft. For downlinks, all of these potentially come into play, and the effects of the atmosphere, including turbulence, scattering, and absorption contribute as well. Methods for accounting for each of these are presented. Specific examples are presented to illustrate the relative contributions of each source for various link geometries.
Effect of erythrocyte aggregation on optical transmission of blood
NASA Astrophysics Data System (ADS)
Shvartsman, L. D.; Fine, I.
2007-02-01
We present here a bird-eye view of time-dependent optical transmission of blood in red-near infrared spectral range. This issue is of the key importance both for fundamental understanding and for various applications connected with non-invasive optical blood analysis. A number of experiments measuring kinetics of blood transmission in the case of natural heart pulsations and of artificial kinetics following over-systolic occlusion is reviewed. The comprehensive theoretical approach has to consider scattering-associated mechanism rather than the widely accepted absorption-associated one. Light scattering occurs on RBC aggregates. The size of aggregates and their shape change in time due to blood flow variations. It results in the corresponding changes of optical transmission.
van den Berg, Thomas J T P
2017-05-01
The effect of cataract and other media opacities on functional vision is typically assessed clinically using visual acuity. In both clinical and basic research, straylight (the functional result of light scattering in the eye) is commonly measured. The purpose of the present study was to determine the link between these two measures: is visual acuity in cataract and other media opacities related to straylight? Interdependence between acuity and straylight is addressed from three different points of view: (1) Methodological: can acuity differences affect the measurement value of straylight, and vice versa? (2) Basic optics: does the optical process of light scattering in the human eye affect both straylight and visual acuity? (3) Statistical: how strongly are acuity and straylight correlated in the practice of important clinical conditions? Experimental and theoretical aspects will be considered, with a focus on normal ageing and cataract formation. (1) Methodological: testing potential effects of acuity, artificially manipulated with positive trial lenses, showed no effect on measured straylight values. Since light scattering in the eye involves a low percentage of the light and has large angular spreading, contrast reduction due to straylight is limited, resulting in virtually absent acuity effects. (2) Basic optics: light scattering from the human donor eye lens is found to have virtually no effect in the centre of the point-spread-function, also for cataractous lenses, resulting in virtually absent acuity effects. (3) Statistical: literature data on straylight and visual acuity show a weak correlation for the important groups of normal ageing and cataract populations. The point-spread-function of the normal ageing and cataractous human eye is built upon two rather independent basic parts. Aberrations control the central peak. Light scattering controls the periphery from about 1° onwards. The way acuity and straylight are measured ensures no confounding between them. Statistically within the normal ageing and cataract populations, visual acuity and straylight vary quite independently from each other. Visual acuity losses with cataract and other media opacities are not due to straylight, but caused by aberrations and micro-aberrations. Straylight defines disability glare, and causes symptoms of glare, haloes, hazy vision etc. Overall, visual acuity and straylight are rather independent aspects of quality of vision. © 2017 The Author Ophthalmic & Physiological Optics © 2017 The College of Optometrists.
Speckle dynamics under ergodicity breaking
NASA Astrophysics Data System (ADS)
Sdobnov, Anton; Bykov, Alexander; Molodij, Guillaume; Kalchenko, Vyacheslav; Jarvinen, Topias; Popov, Alexey; Kordas, Krisztian; Meglinski, Igor
2018-04-01
Laser speckle contrast imaging (LSCI) is a well-known and versatile approach for the non-invasive visualization of flows and microcirculation localized in turbid scattering media, including biological tissues. In most conventional implementations of LSCI the ergodic regime is typically assumed valid. However, most composite turbid scattering media, especially biological tissues, are non-ergodic, containing a mixture of dynamic and static centers of light scattering. In the current study, we examined the speckle contrast in different dynamic conditions with the aim of assessing limitations in the quantitative interpretation of speckle contrast images. Based on a simple phenomenological approach, we introduced a coefficient of speckle dynamics to quantitatively assess the ratio of the dynamic part of a scattering medium to the static one. The introduced coefficient allows one to distinguish real changes in motion from the mere appearance of static components in the field of view. As examples of systems with static/dynamic transitions, thawing and heating of Intralipid samples were studied by the LSCI approach.
Optical properties (bidirectional reflectance distribution function) of shot fabric.
Lu, R; Koenderink, J J; Kappers, A M
2000-11-01
To study the optical properties of materials, one needs a complete set of the angular distribution functions of surface scattering from the materials. Here we present a convenient method for collecting a large set of bidirectional reflectance distribution function (BRDF) samples in the hemispherical scattering space. Material samples are wrapped around a right-circular cylinder and irradiated by a parallel light source, and the scattered radiance is collected by a digital camera. We tilted the cylinder around its center to collect the BRDF samples outside the plane of incidence. This method can be used with materials that have isotropic and anisotropic scattering properties. We demonstrate this method in a detailed investigation of shot fabrics. The warps and the fillings of shot fabrics are dyed different colors so that the fabric appears to change color at different viewing angles. These color-changing characteristics are found to be related to the physical and geometrical structure of shot fabric. Our study reveals that the color-changing property of shot fabrics is due mainly to an occlusion effect.
Optical memory effect from polarized Laguerre-Gaussian light beam in light-scattering turbid media
NASA Astrophysics Data System (ADS)
Shumyatsky, Pavel; Milione, Giovanni; Alfano, Robert R.
2014-06-01
Propagation effects of polarized Laguerre-Gaussian light with different orbital angular momentum (L) in turbid media are described. The optical memory effect in scattering media consisting of small and large size (compared to the wavelength) scatterers is investigated for scattered polarized light. Imaging using polarized laser modes with a varying orbital strength L-parameter was performed. The backscattered image quality (contrast) was enhanced by more than an order of magnitude using circularly polarized light when the concentration of scatterers was close to invisibility of the object.
Stimulated concentration (diffusion) light scattering on nanoparticles in a liquid suspension
NASA Astrophysics Data System (ADS)
Burkhanov, I. S.; Krivokhizha, S. V.; Chaikov, L. L.
2016-06-01
A nonlinear growth of the light scattering intensity has been observed and the frequency shift of the spectral line of scattered light has been measured in light backscattered in suspensions of diamond and latex nanoparticles in water. The shift corresponds to the HWHM of the line of spontaneous scattering on particles. We may conclude that there exists stimulated concentration (diffusion) light scattering on variations of the particle concentration, which is also called the stimulated Mie scattering. In a fibre probe scheme, the growth of the shift of the scattered spectral line is observed with an increase in the exciting beam power. The variation of the frequency shift with an increase in the exciting power is explained by convection in liquid.
Global Plasmaspheric Imaging: A New "Light" Focusing on Familiar Questions
NASA Technical Reports Server (NTRS)
Adrian, M. L.; Six, N. Frank (Technical Monitor)
2002-01-01
Until recently plasmaspheric physics, for that matter, magnetospheric physics as a whole, has relied primarily on single point in-situ measurement, theory, modeling, and a considerable amount of extrapolation in order to envision the global structure of the plasmasphere. This condition changed with the launch of the IMAGE satellite in March 2000. Using the Extreme Ultraviolet (EUV) imager on WAGE, we can now view the global structure of the plasmasphere bathed in the glow of resonantly scattered 30.4 nm radiation allowing the space physics community to view the dynamics of this global structure as never before. This talk will: (1) define the plasmasphere from the perspective of plasmaspheric physics prior to March 2000; (2) present a review of EUV imaging optics and the IMAGE mission; and focus on efforts to understand an old and familiar feature of plasmaspheric physics, embedded plasmaspheric density troughs, in this new global light with the assistance of forward modeling.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Gong, Tao; Research Center of Laser Fusion, China Academy of Engineering Physics, Mianyang, Sichuan 621900; Zheng, Jian, E-mail: jzheng@ustc.edu.cn
2016-06-15
A 2D cylindrically symmetric model with inclusion of both diffraction and self-focus effects is developed to deal with the stimulated scattering processes of a single hotspot. The calculated results show that the transverse distribution of the scattered light is sensitive to the longitudinal profiles of the plasma parameters. The analysis of the evolution of the scattered light indicates that it is the frequency mismatch of coupling due to the inhomogeneity of plasmas that determines the transverse distribution of the scattered light.
Wide-field optical detection of nanoparticles using on-chip microscopy and self-assembled nanolenses
NASA Astrophysics Data System (ADS)
Mudanyali, Onur; McLeod, Euan; Luo, Wei; Greenbaum, Alon; Coskun, Ahmet F.; Hennequin, Yves; Allier, Cédric P.; Ozcan, Aydogan
2013-03-01
The direct observation of nanoscale objects is a challenging task for optical microscopy because the scattering from an individual nanoparticle is typically weak at optical wavelengths. Electron microscopy therefore remains one of the gold standard visualization methods for nanoparticles, despite its high cost, limited throughput and restricted field-of-view. Here, we describe a high-throughput, on-chip detection scheme that uses biocompatible wetting films to self-assemble aspheric liquid nanolenses around individual nanoparticles to enhance the contrast between the scattered and background light. We model the effect of the nanolens as a spatial phase mask centred on the particle and show that the holographic diffraction pattern of this effective phase mask allows detection of sub-100 nm particles across a large field-of-view of >20 mm2. As a proof-of-concept demonstration, we report on-chip detection of individual polystyrene nanoparticles, adenoviruses and influenza A (H1N1) viral particles.
Wide-field optical detection of nanoparticles using on-chip microscopy and self-assembled nanolenses
Mudanyali, Onur; McLeod, Euan; Luo, Wei; Greenbaum, Alon; Coskun, Ahmet F.; Hennequin, Yves; Allier, Cédric P.; Ozcan, Aydogan
2013-01-01
The direct observation of nanoscale objects is a challenging task for optical microscopy because the scattering from an individual nanoparticle is typically weak at optical wavelengths. Electron microscopy therefore remains one of the gold standard visualization methods for nanoparticles, despite its high cost, limited throughput and restricted field-of-view. Here, we describe a high-throughput, on-chip detection scheme that uses biocompatible wetting films to self-assemble aspheric liquid nanolenses around individual nanoparticles to enhance the contrast between the scattered and background light. We model the effect of the nanolens as a spatial phase mask centred on the particle and show that the holographic diffraction pattern of this effective phase mask allows detection of sub-100 nm particles across a large field-of-view of >20 mm2. As a proof-of-concept demonstration, we report on-chip detection of individual polystyrene nanoparticles, adenoviruses and influenza A (H1N1) viral particles. PMID:24358054
Intraocular light scatter, reflections, fluorescence and absorption: what we see in the slit lamp.
van den Berg, Thomas J T P
2018-01-01
Much knowledge has been collected over the past 20 years about light scattering in the eye- in particular in the eye lens- and its visual effect, called straylight. It is the purpose of this review to discuss how these insights can be applied to understanding the slit lamp image. The slit lamp image mainly results from back scattering, whereas the effects on vision result mainly from forward scatter. Forward scatter originates from particles of about wavelength size distributed throughout the lens. Most of the slit lamp image originates from small particle scatter (Rayleigh scatter). For a population of middle aged lenses it will be shown that both these scatter components remove around 10% of the light from the direct beam. For slit lamp observation close to the reflection angles, zones of discontinuity (Wasserspalten) at anterior and posterior parts of the lens show up as rough surface reflections. All these light scatter effects increase with age, but the correlations with age, and also between the different components, are weak. For retro-illumination imaging it will be argued that the density or opacity seen in areas of cortical or posterior subcapsular cataract show up because of light scattering, not because of light loss. NOTES: (1) Light scatter must not be confused with aberrations. Light penetrating the eye is divided into two parts: a relatively small part is scattered, and removed from the direct beam. Most of the light is not scattered, but continues as the direct beam. This non-scattered part is the basis for functional imaging, but its quality is under the control of aberrations. Aberrations deflect light mainly over small angles (<1°), whereas light scatter is important because of the straylight effects over large angles (>1°), causing problems like glare and hazy vision. (2) The slit lamp image in older lenses and nuclear cataract is strongly influenced by absorption. However, this effect is greatly exaggerated by the light path lengths concerned. This obviates proper judgement of the functional importance of absorption, and hinders the appreciation of the Rayleigh nature of what is seen in the slit lamp image. © 2017 The Authors Ophthalmic & Physiological Optics © 2017 The College of Optometrists.
Tans, Petrus P.; Lashof, Daniel A.
1986-01-01
A device for determining the relative composition of a sample of a gas by comparison of the Raman-scattered light of the sample with that of a known gas comprising: a means for passing a single light source through the unknown and the known gases, choppers to alternate the Raman-scattered light into a common light detection and measuring system, optical fiber networks for spatially mixing the resulting Raman scattered light from each sample and directing the mixed light to selective detectors, and a compiler to record the light intensity of each wavelength of Raman-scattered light as a function of the sample from which it originated.
Soos, Miroslav; Lattuada, Marco; Sefcik, Jan
2009-11-12
In this work we studied the effect of intracluster multiple-light scattering on the scattering properties of a population of fractal aggregates. To do so, experimental data of diffusion-limited aggregation for three polystyrene latexes with similar surface properties but different primary particle diameters (equal to 118, 420, and 810 nm) were obtained by static light scattering and by means of a spectrophotometer. In parallel, a population balance equation (PBE) model, which takes into account the effect of intracluster multiple-light scattering by solving the T-matrix and the mean-field version of T-matrix, was formulated and validated against time evolution of the root mean radius of gyration,
Static and dynamic light scattering by red blood cells: A numerical study.
Mauer, Johannes; Peltomäki, Matti; Poblete, Simón; Gompper, Gerhard; Fedosov, Dmitry A
2017-01-01
Light scattering is a well-established experimental technique, which gains more and more popularity in the biological field because it offers the means for non-invasive imaging and detection. However, the interpretation of light-scattering signals remains challenging due to the complexity of most biological systems. Here, we investigate static and dynamic scattering properties of red blood cells (RBCs) using two mesoscopic hydrodynamics simulation methods-multi-particle collision dynamics and dissipative particle dynamics. Light scattering is studied for various membrane shear elasticities, bending rigidities, and RBC shapes (e.g., biconcave and stomatocyte). Simulation results from the two simulation methods show good agreement, and demonstrate that the static light scattering of a diffusing RBC is not very sensitive to the changes in membrane properties and moderate alterations in cell shapes. We also compute dynamic light scattering of a diffusing RBC, from which dynamic properties of RBCs such as diffusion coefficients can be accessed. In contrast to static light scattering, the dynamic measurements can be employed to differentiate between the biconcave and stomatocytic RBC shapes and generally allow the differentiation based on the membrane properties. Our simulation results can be used for better understanding of light scattering by RBCs and the development of new non-invasive methods for blood-flow monitoring.
Static and dynamic light scattering by red blood cells: A numerical study
Mauer, Johannes; Peltomäki, Matti; Poblete, Simón; Gompper, Gerhard
2017-01-01
Light scattering is a well-established experimental technique, which gains more and more popularity in the biological field because it offers the means for non-invasive imaging and detection. However, the interpretation of light-scattering signals remains challenging due to the complexity of most biological systems. Here, we investigate static and dynamic scattering properties of red blood cells (RBCs) using two mesoscopic hydrodynamics simulation methods—multi-particle collision dynamics and dissipative particle dynamics. Light scattering is studied for various membrane shear elasticities, bending rigidities, and RBC shapes (e.g., biconcave and stomatocyte). Simulation results from the two simulation methods show good agreement, and demonstrate that the static light scattering of a diffusing RBC is not very sensitive to the changes in membrane properties and moderate alterations in cell shapes. We also compute dynamic light scattering of a diffusing RBC, from which dynamic properties of RBCs such as diffusion coefficients can be accessed. In contrast to static light scattering, the dynamic measurements can be employed to differentiate between the biconcave and stomatocytic RBC shapes and generally allow the differentiation based on the membrane properties. Our simulation results can be used for better understanding of light scattering by RBCs and the development of new non-invasive methods for blood-flow monitoring. PMID:28472125
Development of wide-angle 2D light scattering static cytometry
NASA Astrophysics Data System (ADS)
Xie, Linyan; Liu, Qiao; Shao, Changshun; Su, Xuantao
2016-10-01
We have recently developed a 2D light scattering static cytometer for cellular analysis in a label-free manner, which measures side scatter (SSC) light in the polar angular range from 79 to 101 degrees. Compared with conventional flow cytometry, our cytometric technique requires no fluorescent labeling of the cells, and static cytometry measurements can be performed without flow control. In this paper we present an improved label-free static cytometer that can obtain 2D light scattering patterns in a wider angular range. By illuminating the static microspheres on chip with a scanning optical fiber, wide-angle 2D light scattering patterns of single standard microspheres with a mean diameter of 3.87 μm are obtained. The 2D patterns of 3.87 μm microspheres contain both large-angle forward scatter (FSC) and SSC light in the polar angular range from 40 to 100 degrees, approximately. Experimental 2D patterns of 3.87 μm microspheres are in good agreement with Mie theory simulated ones. The wide-angle light scattering measurements may provide a better resolution for particle analysis as compared with the SSC measurements. Two dimensional light scattering patterns of HL-60 human acute leukemia cells are obtained by using our static cytometer. Compared with SSC 2D light scattering patterns, wide-angle 2D patterns contain richer information of the HL-60 cells. The obtaining of 2D light scattering patterns in a wide angular range could help to enhance the capabilities of our label-free static cytometry for cell analysis.
NASA Astrophysics Data System (ADS)
Greeley, A.; Kurtz, N. T.; Neumann, T.; Cook, W. B.; Markus, T.
2016-12-01
Photon counting laser altimeters such as MABEL (Multiple Altimeter Beam Experimental Lidar) - a single photon counting simulator for ATLAS (Advanced Topographical Laser Altimeter System) - use individual photons with visible wavelengths to measure their range to target surfaces. ATLAS, the sole instrument on NASA's upcoming ICESat-2 mission, will provide scientists a view of Earth's ice sheets, glaciers, and sea ice with unprecedented detail. Precise calibration of these instruments is needed to understand rapidly changing parameters such as sea ice freeboard, and to measure optical properties of surfaces like snow covered ice sheets using subsurface scattered photons. Photons that travel through snow, ice, or water before scattering back to an altimeter receiving system travel farther than photons taking the shortest path between the observatory and the target of interest. These delayed photons produce a negative elevation bias relative to photons scattered directly off these surfaces. We use laboratory measurements of snow surfaces using a flight-tested laser altimeter (MABEL), and Monte Carlo simulations of backscattered photons from snow to estimate elevation biases from subsurface scattered photons. We also use these techniques to demonstrate the ability to retrieve snow surface properties like snow grain size.
Time of flight imaging through scattering environments (Conference Presentation)
NASA Astrophysics Data System (ADS)
Le, Toan H.; Breitbach, Eric C.; Jackson, Jonathan A.; Velten, Andreas
2017-02-01
Light scattering is a primary obstacle to imaging in many environments. On small scales in biomedical microscopy and diffuse tomography scenarios scattering is caused by tissue. On larger scales scattering from dust and fog provide challenges to vision systems for self driving cars and naval remote imaging systems. We are developing scale models for scattering environments and investigation methods for improved imaging particularly using time of flight transient information. With the emergence of Single Photon Avalanche Diode detectors and fast semiconductor lasers, illumination and capture on picosecond timescales are becoming possible in inexpensive, compact, and robust devices. This opens up opportunities for new computational imaging techniques that make use of photon time of flight. Time of flight or range information is used in remote imaging scenarios in gated viewing and in biomedical imaging in time resolved diffuse tomography. In addition spatial filtering is popular in biomedical scenarios with structured illumination and confocal microscopy. We are presenting a combination analytical, computational, and experimental models that allow us develop and test imaging methods across scattering scenarios and scales. This framework will be used for proof of concept experiments to evaluate new computational imaging methods.
Schoenfeld, Andreas A; Poppinga, Daniela; Harder, Dietrich; Doerner, Karl-Joachim; Poppe, Bjoern
2014-07-07
Optical experiments and theoretical considerations have been undertaken in order to understand the causes of the 'orientation effect' and the 'parabola effect', the artefacts impairing the desired light absorption measurement on radiochromic EBT3 films with flatbed scanners. EBT3 films exposed to doses up to 20.9 Gy were scanned with an Epson Expression 10000XL flatbed scanner in landscape and portrait orientation. The horizontally and vertically polarized light components of the scanner were determined, and another Epson Expression 10000XL flatbed scanner was disassembled to examine its optical components. The optical properties of exposed and unexposed EBT3 films were studied with incident polarized and unpolarized white light, and the transmitted red light was investigated for its polarization and scattering properties including the distribution of the scattering angles. Neutral density filters were studied for comparison. Guidance was sought from the theory of light scattering from rod-like macromolecular structures. The drastic dose-dependent variation of the transmitted total light current as function of the orientation of front and rear polarizers, interpreted by light scattering theory, shows that the radiation-induced polymerization of the monomers of EBT3 films produces light scattering oscillators preferably polarized at right angles with the coating direction of the film. The directional distribution of the scattered light is partly anisotropic, with a preferred scattering plane at right angles with the coating direction, indicating light scattering from stacks of coherently vibrating oscillators piled up along the monomer crystals. The polyester carrier film also participates in these effects. The 'orientation' and 'parabola' artefacts due to flatbed scanning of radiochromic films can be explained by the interaction of the polarization-dependent and anisotropic light scattering from exposed and unexposed EBT3 films with the quantitative difference between the scanner's horizontally and vertically polarized light supply and with the limited directional acceptance of the scanner's light recording system.
NASA Astrophysics Data System (ADS)
Kassamakov, Ivan; Maconi, Göran; Penttilä, Antti; Helander, Petteri; Gritsevich, Maria; Puranen, Tuomas; Salmi, Ari; Hæggström, Edward; Muinonen, Karri
2018-02-01
We present the design of a novel scatterometer for precise measurement of the angular Mueller matrix profile of a mm- to µm-sized sample held in place by sound. The scatterometer comprises a tunable multimode Argon-krypton laser (with possibility to set 1 of the 12 wavelengths in visible range), linear polarizers, a reference photomultiplier tube (PMT) for monitoring the beam intensity, and a micro-PMT module mounted radially towards the sample at an adjustable radius. The measurement angle is controlled by a motor-driven rotation stage with an accuracy of 15'. The system is fully automated using LabVIEW, including the FPGA-based data acquisition and the instrument's user interface. The calibration protocol ensures accurate measurements by using a control sphere sample (diameter 3 mm, refractive index of 1.5) fixed first on a static holder followed by accurate multi-wavelength measurements of the same sample levitated ultrasonically. To demonstrate performance of the scatterometer, we conducted detailed measurements of light scattered by a particle derived from the Chelyabinsk meteorite, as well as planetary analogue materials. The measurements are the first of this kind, since they are obtained using controlled spectral angular scattering including linear polarization effects, for arbitrary shaped objects. Thus, our novel approach permits a non-destructive, disturbance-free measurement with control of the orientation and location of the scattering object.
Light intensity modulation by coccoliths of Emiliania huxleyi as a micro-photo-regulator.
Mizukawa, Yuri; Miyashita, Yuito; Satoh, Manami; Shiraiwa, Yoshihiro; Iwasaka, Masakazu
2015-09-01
In this study, we present experimental evidence showing that coccoliths have light-scattering anisotropy that contributes to a possible control of solar light exposure in the ocean. Changing the angle between the incident light and an applied magnetic field causes differences in the light-scattering intensities of a suspension of coccoliths isolated from Emiliania huxleyi. The magnetic field effect is induced by the diamagnetic torque force directing the coccolith radial plane perpendicular to the applied magnetic fields at 400 to 500 mT. The developed technique reveals the light-scattering anisotropies in the 3-μm-diameter floating coccoliths by orienting themselves in response to the magnetic fields. The detached coccolith scatters radially the light incident to its radial plane. The experimental results on magnetically oriented coccoliths show that an individual coccolith has a specific direction of light scattering, although the possible physiological effect of the coccolith remains for further study, focusing on the light-scattering anisotropies of coccoliths on living cells.
Light intensity modulation by coccoliths of Emiliania huxleyi as a micro-photo-regulator
NASA Astrophysics Data System (ADS)
Mizukawa, Yuri; Miyashita, Yuito; Satoh, Manami; Shiraiwa, Yoshihiro; Iwasaka, Masakazu
2015-09-01
In this study, we present experimental evidence showing that coccoliths have light-scattering anisotropy that contributes to a possible control of solar light exposure in the ocean. Changing the angle between the incident light and an applied magnetic field causes differences in the light-scattering intensities of a suspension of coccoliths isolated from Emiliania huxleyi. The magnetic field effect is induced by the diamagnetic torque force directing the coccolith radial plane perpendicular to the applied magnetic fields at 400 to 500 mT. The developed technique reveals the light-scattering anisotropies in the 3-μm-diameter floating coccoliths by orienting themselves in response to the magnetic fields. The detached coccolith scatters radially the light incident to its radial plane. The experimental results on magnetically oriented coccoliths show that an individual coccolith has a specific direction of light scattering, although the possible physiological effect of the coccolith remains for further study, focusing on the light-scattering anisotropies of coccoliths on living cells.
Improved Optics For Quasi-Elastic Light Scattering
NASA Technical Reports Server (NTRS)
Cheung, Harry Michael
1995-01-01
Improved optical train devised for use in light-scattering measurements of quasi-elastic light scattering (QELS) and laser spectroscopy. Measurements performed on solutions, microemulsions, micellular solutions, and colloidal dispersions. Simultaneous measurements of total intensity and fluctuations in total intensity of light scattered from sample at various angles provides data used, in conjunction with diffusion coefficients, to compute sizes of particles in sample.
NASA Astrophysics Data System (ADS)
Roy, Sanchita; Barua, Nilakshi; Buragohain, Alak K.; Ahmed, Gazi A.
2013-03-01
Investigations on treatment of ZnO nanoparticles on Staphylococcus aureus MTCC 737 strain was essentially made by using standard biochemical method. The anti-microbial assay against S. aureus, and time kill assay revealed the anti-bacterial activity of ZnO nanoparticles. We have substantiated this property of ZnO nanoparticles and light depolarization property by using light scattering tool. Light scattering measurements were carried out for ZnO, S. aureus, and ZnO treated S. aureus as a function of scattering angle at 543.5 and 632.8 nm wavelengths. This was done in order to find the scattering profile of the consequent product after the action of ZnO nanoparticles on bacteria by means of light scattering tool. S. aureus treated with ZnO nanoparticles showed closer agreement of the scattering profiles at both the wavelengths, however, the scattering profiles of ZnO nanoparticles and untreated S. aureus significantly varied for the two different laser wavelengths. It was also observed that there was higher intensity of scattering from all S. aureus treated with ZnO particles compared to the untreated ones. In our work, we have studied ZnO nanoparticles and the possibility of observing its anti-bacterial activity by using light scattering tool.
Design of fiber optic probes for laser light scattering
NASA Technical Reports Server (NTRS)
Dhadwal, Harbans S.; Chu, Benjamin
1989-01-01
A quantitative analysis is presented of the role of optical fibers in laser light scattering. Design of a general fiber optic/microlens probe by means of ray tracing is described. Several different geometries employing an optical fiber of the type used in lightwave communications and a graded index microlens are considered. Experimental results using a nonimaging fiber optic detector probe show that due to geometrical limitations of single mode fibers, a probe using a multimode optical fiber has better performance, for both static and dynamic measurements of the scattered light intensity, compared with a probe using a single mode fiber. Fiber optic detector probes are shown to be more efficient at data collection when compared with conventional approaches to measurements of the scattered laser light. Integration of fiber optic detector probes into a fiber optic spectrometer offers considerable miniaturization of conventional light scattering spectrometers, which can be made arbitrarily small. In addition static and dynamic measurements of scattered light can be made within the scattering cell and consequently very close to the scattering center.
Morris, Caleb; Werner, Liliana; Barra, Daniel; Liu, Erica; Stallings, Shannon; Floyd, Anne
2014-01-01
To evaluate light scattering and light transmittance in cadaver eye-explanted intraocular lenses (IOLs) manufactured from different materials. John A. Moran Eye Center, University of Utah, Salt Lake City, Utah, USA. Experimental study. Forty-nine pseudophakic cadaver eyes were selected according to IOL material/type and implantation duration, and the IOLs were explanted. Hydrophobic acrylic, hydrophilic acrylic, poly(methyl methacrylate) (PMMA), and silicone IOLs were included. Gross and light microscopy was performed for all IOLs. Light scattering was measured with an EAS 1000 Scheimpflug camera, and light transmittance was assessed using a Lambda 35 UV/Vis spectrophotometer (single-beam configuration with an RSA PE-20 integrating sphere). Analyses were performed at room temperature in the hydrated state and compared with analyses of controls. The highest levels of surface light scattering were measured for 3-piece hydrophobic acrylic, which was also the IOL type with the longest implantation duration among the Acrysof hydrophobic acrylic IOLs. Hydrophilic acrylic, PMMA, and silicone IOLs exhibited relatively low light-scattering levels. The lowest light-scattering levels were observed with PMMA IOLs (1-piece looped and 3-piece) and plate silicone IOLs, which represent the IOL types with the longest implantation duration in this series. Light transmittance values measured for all IOL types appeared to be similar to the values of the corresponding control IOLs. The phenomenon of surface light scattering (nanoglistenings) is more particularly related to hydrophobic acrylic IOLs and increases with implantation time. No significant effect of surface light scattering on IOL light transmittance was found. Copyright © 2013 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.
Spectroscopic limits to an extragalactic far-ultraviolet background.
Martin, C; Hurwitz, M; Bowyer, S
1991-10-01
We use a spectrum of the lowest intensity diffuse far-ultraviolet background obtained from a series of observations in a number of celestial view directions to constrain the properties of the extragalactic FUV background. The mean continuum level, IEG = 280 +/- 35 photons cm-2 s-1 angstrom-1 sr-1, was obtained in a direction with very low H I column density, and this represents a firm upper limit to any extragalactic background in the 1400-1900 angstroms band. Previous work has demonstrated that the far-ultraviolet background includes (depending on a view direction) contributions from dust-scattered Galactic light, high-ionization emission lines, two-photon emission from H II, H2 fluorescence, and the integrated light of spiral galaxies. We find no evidence in the spectrum of line or continuum features that would signify additional extragalactic components. Motivated by the observation of steep BJ and U number count distributions, we have made a detailed comparison of galaxy evolution models to optical and UV data. We find that the observations are difficult to reconcile with a dominant contribution from unclustered, starburst galaxies at low redshifts. Our measurement rules out large ionizing fluxes at z = 0, but cannot strongly constrain the QSO background light, which is expected to be 0.5%-4% of IEG. We present improved limits on radiative lifetimes of massive neutrinos. We demonstrated with a simple model that IGM radiation is unlikely to make a significant contribution to IEG. Since dust scattering could produce a significant part of the continuum in this lowest intensity spectrum, we carried out a series of tests to evaluate this possibility. We find that the spectrum of a nearby target with higher NH I, when corrected for H2 fluorescence, is very similar to the spectrum obtained in the low H I view direction. This is evidence that the majority of the continuum observed at low NH I is also dust reflection, indicating either the existence of a hitherto unidentified dust component, or of a large enhancement in dust scattering efficiency in low-density gas. We also review the effects of an additional dust component on the far-infrared background and on extragalactic FUV observations. We conclude that dust reflection, combined with modest contributions from H II two-photon emission and from the integrated light of late-type galaxies, may account for virtually all of the FUV background in low H I column density directions.
Ahsan, Syed Saad; Pereyra, Brandon; Jung, Erica E; Erickson, David
2014-10-20
Most existing photobioreactors do a poor job of distributing light uniformly due to shading effects. One method by which this could be improved is through the use of internal wave-guiding structures incorporating engineered light scattering schemes. By varying the density of these scatterers, one can control the spatial distribution of light inside the reactor enabling better uniformity of illumination. Here, we compare a number of light scattering schemes and evaluate their ability to enhance biomass accumulation. We demonstrate a design for a gradient distribution of surface scatterers with uniform lateral scattering intensity that is superior for algal biomass accumulation, resulting in a 40% increase in the growth rate.
LADEE UVS Observations of Atoms and Dust in the Lunar Tail
NASA Technical Reports Server (NTRS)
Wooden, Diane H.; Colaprete, Anthony; Cook, Amanda M.; Shirley, Mark H.; Vargo, Kara E.; Elphic, Richard C.; Stubbs, Timothy J.; Glenar, David A.
2014-01-01
The Lunar Atmosphere and Dust Environment Explorer (LADEE) was a lunar orbiter launched in September 2013 that investigated the composition and temporal variation of the tenuous lunar exosphere and dust environment. A major goal of the mission was to characterize the dust exosphere prior to future lunar exploration activities, which may alter the lunar environment. The Ultraviolet/Visible Spectrometer (UVS) onboard LADEE addresses this goal, utilizing two sets of optics: a limbviewing telescope, and a solar-viewing telescope. We report on spectroscopic (approximately 280 - 820 nm) observations viewing down the lunar wake or along the 'lunar tail' from lunar orbit. Prior groundbased studies have observed the emission from neutral sodium atoms extended along the lunar tail, so often this region is referred to as the lunar sodium tail. UVS measurements were made on the dark side of the moon, with the UVS limb-viewing telescope pointed outward in the direction of the Moon's wake (almost anti-sun), during different lunar phases. These UVS observation activities sample a long column and allow the characterization of scattered light from dust and emission lines from atoms in the lunar tail. Observations in this UVS configuration show the largest excess of scattered blue light in our data set, indicative of the presence of small dust grains in the tail. Once lofted, nanoparticles may become charged and picked up by the solar wind, similar to the phenomena witnessed above Enceladus's northern hemisphere or by the STEREO/WAVES instrument while close to Earth's orbit. The UVS data show that small dust grains as well as atoms become entrained in the lunar tail.
ASHI: An All Sky Heliospheric Imager for Viewing Thomson-Scattered Light
NASA Astrophysics Data System (ADS)
Buffington, A.; Jackson, B. V.; Yu, H. S.; Hick, P. P.; Bisi, M. M.
2017-12-01
We have developed, and are now making a detailed design for an All-Sky Heliospheric Imager (ASHI), to fly on future deep-space missions. ASHI's principal long-term objective is acquisition of a precision photometric map of the inner heliosphere as viewed from deep space. Photometers on the twin Helios spacecraft, the Solar Mass Ejection Imager (SMEI) upon the Coriolis satellite, and the Heliospheric Imagers (HIs) upon the Solar-TErrestrial RElations Observatory (STEREO) twin spacecraft, all indicate an optimum instrument design for visible-light Thomson-scattering observations. This design views a hemisphere of sky starting a few degrees from the Sun. Two imagers can cover almost all of the whole sky. A key photometric specification for ASHI is 0.1% differential photometry: this enables the three dimensional reconstruction of density starting from near the Sun and extending outward. SMEI analyses have demonstrated the success of this technique: when employed by ASHI, this will provide an order of magnitude better resolution in 3-D density over time. We augment this analysis to include velocity, and these imagers deployed in deep space can thus provide high-resolution comparisons both of direct in-situ density and velocity measurements to remote observations of solar wind structures. In practice we find that the 3-D velocity determinations provide the best tomographic timing depiction of heliospheric structures. We discuss the simple concept behind this, and present recent progress in the instrument design, and its expected performance specifications. A preliminary balloon flight of an ASHI prototype is planned to take place next Summer.
Phase Curves of Nix and Hydra from the New Horizons Imaging Cameras
NASA Astrophysics Data System (ADS)
Verbiscer, Anne J.; Porter, Simon B.; Buratti, Bonnie J.; Weaver, Harold A.; Spencer, John R.; Showalter, Mark R.; Buie, Marc W.; Hofgartner, Jason D.; Hicks, Michael D.; Ennico-Smith, Kimberly; Olkin, Catherine B.; Stern, S. Alan; Young, Leslie A.; Cheng, Andrew; (The New Horizons Team
2018-01-01
NASA’s New Horizons spacecraft’s voyage through the Pluto system centered on 2015 July 14 provided images of Pluto’s small satellites Nix and Hydra at viewing angles unattainable from Earth. Here, we present solar phase curves of the two largest of Pluto’s small moons, Nix and Hydra, observed by the New Horizons LOng Range Reconnaissance Imager and Multi-spectral Visible Imaging Camera, which reveal the scattering properties of their icy surfaces in visible light. Construction of these solar phase curves enables comparisons between the photometric properties of Pluto’s small moons and those of other icy satellites in the outer solar system. Nix and Hydra have higher visible albedos than those of other resonant Kuiper Belt objects and irregular satellites of the giant planets, but not as high as small satellites of Saturn interior to Titan. Both Nix and Hydra appear to scatter visible light preferentially in the forward direction, unlike most icy satellites in the outer solar system, which are typically backscattering.
Multiple-Fiber-Optic Probe For Light-Scattering Measurements
NASA Technical Reports Server (NTRS)
Dhadwal, Harbans Singh; Ansari, Rafat R.
1996-01-01
Multiple-fiber-optical probe developed for use in measuring light scattered at various angles from specimens of materials. Designed for both static and dynamic light-scattering measurements of colloidal dispersions. Probe compact, rugged unit containing no moving parts and remains stationary during operation. Not restricted to operation in controlled, research-laboratory environment. Positioned inside or outside light-scattering chamber. Provides simultaneous measurements at small angular intervals over range of angles, made to include small scattering angles by orienting probe in appropriate direction.
Light-front representation of chiral dynamics in peripheral transverse densities
Granados, Carlos G.; Weiss, Christian
2015-07-31
The nucleon's electromagnetic form factors are expressed in terms of the transverse densities of charge and magnetization at fixed light-front time. At peripheral transverse distances b = O(M_pi^{-1}) the densities are governed by chiral dynamics and can be calculated model-independently using chiral effective field theory (EFT). We represent the leading-order chiral EFT results for the peripheral transverse densities as overlap integrals of chiral light-front wave functions, describing the transition of the initial nucleon to soft pion-nucleon intermediate states and back. The new representation (a) explains the parametric order of the peripheral transverse densities; (b) establishes an inequality between the spin-independentmore » and -dependent densities; (c) exposes the role of pion orbital angular momentum in chiral dynamics; (d) reveals a large left-right asymmetry of the current in a transversely polarized nucleon and suggests a simple interpretation. The light-front representation enables a first-quantized, quantum-mechanical view of chiral dynamics that is fully relativistic and exactly equivalent to the second-quantized, field-theoretical formulation. It relates the charge and magnetization densities measured in low-energy elastic scattering to the generalized parton distributions probed in peripheral high-energy scattering processes. The method can be applied to nucleon form factors of other operators, e.g. the energy-momentum tensor.« less
Are non-linearity effects of absorption important for MAX-DOAS observations?
NASA Astrophysics Data System (ADS)
Pukite, Janis; Wang, Yang; Wagner, Thomas
2017-04-01
For scattered light observations the absorption optical depth depends non-linearly on the trace gas concentrations if their absorption is strong. This is the case because the Beer-Lambert law is generally not applicable for scattered light measurements due to many (i.e. more than one) light paths contributing to the measurement. While in many cases a linear approximation can be made, for scenarios with strong absorption non-linear effects cannot always be neglected. This is especially the case for observation geometries with spatially extended and diffuse light paths, especially in satellite limb geometry but also for nadir measurements as well. Fortunately the effects of non-linear effects can be quantified by means of expanding the radiative transfer equation in a Taylor series with respect to the trace gas absorption coefficients. Herewith if necessary (1) the higher order absorption structures can be described as separate fit parameters in the DOAS fit and (2) the algorithm constraints of retrievals of VCDs and profiles can be improved by considering higher order sensitivity parameters. In this study we investigate the contribution of the higher order absorption structures for MAX-DOAS observation geometry for different atmospheric and ground properties (cloud and aerosol effects, trace gas amount, albedo) and geometry (different Sun and viewing angles).
NASA Astrophysics Data System (ADS)
Jeong, Seungwon; Lee, Ye-Ryoung; Choi, Wonjun; Kang, Sungsam; Hong, Jin Hee; Park, Jin-Sung; Lim, Yong-Sik; Park, Hong-Gyu; Choi, Wonshik
2018-05-01
The efficient delivery of light energy is a prerequisite for the non-invasive imaging and stimulating of target objects embedded deep within a scattering medium. However, the injected waves experience random diffusion by multiple light scattering, and only a small fraction reaches the target object. Here, we present a method to counteract wave diffusion and to focus multiple-scattered waves at the deeply embedded target. To realize this, we experimentally inject light into the reflection eigenchannels of a specific flight time to preferably enhance the intensity of those multiple-scattered waves that have interacted with the target object. For targets that are too deep to be visible by optical imaging, we demonstrate a more than tenfold enhancement in light energy delivery in comparison with ordinary wave diffusion cases. This work will lay a foundation to enhance the working depth of imaging, sensing and light stimulation.
Biological cell classification by multiangle light scattering
Salzman, G.C.; Crowell, J.M.; Mullaney, P.F.
1975-06-03
The specification is directed to an apparatus and method for detecting light scattering from a biological cell. Light, preferably from a coherent source of radiation, intercepts an individual biological cell in a stream of cells passing through the beam. Light scattered from the cell is detected at a selected number of angles between 0 and 90/sup 0/ to the longitudinal axis of the beam with a circular array of light responsive elements which produce signals representative of the intensity of light incident thereon. Signals from the elements are processed to determine the light-scattering pattern of the cell and therefrom its identity.
Confocal non-line-of-sight imaging based on the light-cone transform
NASA Astrophysics Data System (ADS)
O’Toole, Matthew; Lindell, David B.; Wetzstein, Gordon
2018-03-01
How to image objects that are hidden from a camera’s view is a problem of fundamental importance to many fields of research, with applications in robotic vision, defence, remote sensing, medical imaging and autonomous vehicles. Non-line-of-sight (NLOS) imaging at macroscopic scales has been demonstrated by scanning a visible surface with a pulsed laser and a time-resolved detector. Whereas light detection and ranging (LIDAR) systems use such measurements to recover the shape of visible objects from direct reflections, NLOS imaging reconstructs the shape and albedo of hidden objects from multiply scattered light. Despite recent advances, NLOS imaging has remained impractical owing to the prohibitive memory and processing requirements of existing reconstruction algorithms, and the extremely weak signal of multiply scattered light. Here we show that a confocal scanning procedure can address these challenges by facilitating the derivation of the light-cone transform to solve the NLOS reconstruction problem. This method requires much smaller computational and memory resources than previous reconstruction methods do and images hidden objects at unprecedented resolution. Confocal scanning also provides a sizeable increase in signal and range when imaging retroreflective objects. We quantify the resolution bounds of NLOS imaging, demonstrate its potential for real-time tracking and derive efficient algorithms that incorporate image priors and a physically accurate noise model. Additionally, we describe successful outdoor experiments of NLOS imaging under indirect sunlight.
Confocal non-line-of-sight imaging based on the light-cone transform.
O'Toole, Matthew; Lindell, David B; Wetzstein, Gordon
2018-03-15
How to image objects that are hidden from a camera's view is a problem of fundamental importance to many fields of research, with applications in robotic vision, defence, remote sensing, medical imaging and autonomous vehicles. Non-line-of-sight (NLOS) imaging at macroscopic scales has been demonstrated by scanning a visible surface with a pulsed laser and a time-resolved detector. Whereas light detection and ranging (LIDAR) systems use such measurements to recover the shape of visible objects from direct reflections, NLOS imaging reconstructs the shape and albedo of hidden objects from multiply scattered light. Despite recent advances, NLOS imaging has remained impractical owing to the prohibitive memory and processing requirements of existing reconstruction algorithms, and the extremely weak signal of multiply scattered light. Here we show that a confocal scanning procedure can address these challenges by facilitating the derivation of the light-cone transform to solve the NLOS reconstruction problem. This method requires much smaller computational and memory resources than previous reconstruction methods do and images hidden objects at unprecedented resolution. Confocal scanning also provides a sizeable increase in signal and range when imaging retroreflective objects. We quantify the resolution bounds of NLOS imaging, demonstrate its potential for real-time tracking and derive efficient algorithms that incorporate image priors and a physically accurate noise model. Additionally, we describe successful outdoor experiments of NLOS imaging under indirect sunlight.
Tans, P.P.; Lashof, D.A.
1986-12-23
A device is described for determining the relative composition of a sample of a gas by comparison of the Raman-scattered light of the sample with that of a known gas comprising: a means for passing a single light source through the unknown and the known gases, choppers to alternate the Raman-scattered light into a common light detection and measuring system, optical fiber networks for spatially mixing the resulting Raman scattered light from each sample and directing the mixed light to selective detectors, and a compiler to record the light intensity of each wavelength of Raman-scattered light as a function of the sample from which it originated. 6 figs.
Angular-dependent light scattering from cancer cells in different phases of the cell cycle.
Lin, Xiaogang; Wan, Nan; Weng, Lingdong; Zhou, Yong
2017-10-10
Cancer cells in different phases of the cell cycle result in significant differences in light scattering properties. In order to harvest cancer cells in particular phases of the cell cycle, we cultured cancer cells through the process of synchronization. Flow cytometric analysis was applied to check the results of cell synchronization and prepare for light scattering measurements. Angular-dependent light scattering measurements of cancer cells arrested in the G1, S, and G2 phases have been performed. Based on integral calculations for scattering intensities from 5° to 10° and from 110° to 150°, conclusions have been reached. Clearly, the sizes of the cancer cells in different phases of the cell cycle dominated the forward scatter. Accompanying the increase of cell size with the progression of the cell cycle, the forward scattering intensity also increased. Meanwhile, the DNA content of cancer cells in every phase of the cell cycle is responsible for light scattering at large scatter angles. The higher the DNA content of cancer cells was, the greater the positive effect on the high-scattering intensity. As expected, understanding the relationships between the light scattering from cancer cells and cell cycles will aid in the development of cancer diagnoses. Also, it may assist in the guidance of antineoplastic drugs clinically.
Current methods for the size determination of nanomaterials in aqueous suspension include dynamic or static light scattering and electron or atomic force microscopy techniques. Light scattering techniques are limited by poor resolution and the scattering intensity dependence on p...
NASA Technical Reports Server (NTRS)
Andrews, Jane C.; Knowlton, Kelly
2007-01-01
Light pollution has significant adverse biological effects on humans, animals, and plants and has resulted in the loss of our ability to view the stars and planets of the universe. Over half of the U.S. population resides in coastal regions where it is no longer possible to see the stars and planets in the night sky. Forty percent of the entire U.S. population is never exposed to conditions dark enough for their eyes to convert to night vision capabilities. In coastal regions, urban lights shine far out to sea where they are augmented by the output from fishing boat, cruise ship and oil platform floodlights. The proposed candidate solution suggests using HSCs (high sensitivity cameras) onboard the SAC-C and Aquarius/SAC-D satellites to quantitatively evaluate light pollution at high spatial resolution. New products modeled after pre-existing, radiance-calibrated, global nighttime lights products would be integrated into a modified Garstang model where elevation, mountain screening, Rayleigh scattering, Mie scattering by aerosols, and atmospheric extinction along light paths and curvature of the Earth would be taken into account. Because the spatial resolution of the HSCs on SAC-C and the future Aquarius/SAC-D missions is greater than that provided by the DMSP (Defense Meteorological Satellite Program) OLS (Operational Linescan System) or VIIRS (Visible/Infrared Imager/Radiometer Suite), it may be possible to obtain more precise light intensity data for analytical DSSs and the subsequent reduction in coastal light pollution.
Aureole lidar: Design, operation, and comparison with in-situ measurements
NASA Astrophysics Data System (ADS)
Hooper, William P.; Jensen, D. R.
1992-07-01
In 1986, H. Berber and Hooper examined the signals that could be detected by an airborne lidar flying above the marine boundary layer (MBL). One signal (aureole) formed from laser light returned to the receiver after a reflect off the ocean and forward scatter off the aerosol particles appeared to be both detectable and related to the optical depth of the MBL. Now, research has been directed towards developing a practical instrument to measure the aureole and finding an algorithm to use the information. Unlike the lidar backscatter which typically requires a telescope with a narrow field of view (0.5 mrad), the aureole signal occurs over a wide field of view (50 mrad). To accommodate the totally different needs, a standard commercial Cassegrainian telescope was modified to yield a telescope with two focal planes. The secondary mirror was replaced by a lens, whose front surface was half silvered and curved to match the replaced mirror. Light reflecting off the lens focused behind the primary mirror. The back lens surface was curved to allow unreflected light to focus at the natural focus of the primary mirror. This focal plane which is behind the lens has a wide field of view. To calculate an extinction profile, the aureole optical depth estimate is combined with the lidar backscatter profile.
Aureole lidar: Design, operation, and comparison with in-situ measurements
NASA Technical Reports Server (NTRS)
Hooper, William P.; Jensen, D. R.
1992-01-01
In 1986, H. Berber and Hooper examined the signals that could be detected by an airborne lidar flying above the marine boundary layer (MBL). One signal (aureole) formed from laser light returned to the receiver after a reflect off the ocean and forward scatter off the aerosol particles appeared to be both detectable and related to the optical depth of the MBL. Now, research has been directed towards developing a practical instrument to measure the aureole and finding an algorithm to use the information. Unlike the lidar backscatter which typically requires a telescope with a narrow field of view (0.5 mrad), the aureole signal occurs over a wide field of view (50 mrad). To accommodate the totally different needs, a standard commercial Cassegrainian telescope was modified to yield a telescope with two focal planes. The secondary mirror was replaced by a lens, whose front surface was half silvered and curved to match the replaced mirror. Light reflecting off the lens focused behind the primary mirror. The back lens surface was curved to allow unreflected light to focus at the natural focus of the primary mirror. This focal plane which is behind the lens has a wide field of view. To calculate an extinction profile, the aureole optical depth estimate is combined with the lidar backscatter profile.
NASA Astrophysics Data System (ADS)
Hu, Bihe; Bolus, Daniel; Brown, J. Quincy
2018-02-01
Current gold-standard histopathology for cancerous biopsies is destructive, time consuming, and limited to 2D slices, which do not faithfully represent true 3D tumor micro-morphology. Light sheet microscopy has emerged as a powerful tool for 3D imaging of cancer biospecimens. Here, we utilize the versatile dual-view inverted selective plane illumination microscopy (diSPIM) to render digital histological images of cancer biopsies. Dual-view architecture enabled more isotropic resolution in X, Y, and Z; and different imaging modes, such as adding electronic confocal slit detection (eCSD) or structured illumination (SI), can be used to improve degraded image quality caused by background signal of large, scattering samples. To obtain traditional H&E-like images, we used DRAQ5 and eosin (D&E) staining, with 488nm and 647nm laser illumination, and multi-band filter sets. Here, phantom beads and a D&E stained buccal cell sample have been used to verify our dual-view method. We also show that via dual view imaging and deconvolution, more isotropic resolution has been achieved for optical cleared human prostate sample, providing more accurate quantitation of 3D tumor architecture than was possible with single-view SPIM methods. We demonstrate that the optimized diSPIM delivers more precise analysis of 3D cancer microarchitecture in human prostate biopsy than simpler light sheet microscopy arrangements.
Light Scattering by Marine Particles: Modeling with Non-spherical Shapes
2009-01-01
1491−1499, 1994. Gordon, H.R. and Tao Du, Light scattering by nonspherical particles: application to coccoliths detached from Emiliania huxleyi...from Emiliania huxleyi, Applied Optics, (2009). van de Hulst, H.C., 1957. Light Scattering by Small Particles, Wiley. Xu, Yu-lin, and Bo A.S...G.C. Boynton, Light scattering by coccoliths detached from Emiliania huxleyi, Applied Optics, (2009). [submitted, in revision] 6 m = 1.05
DOE Office of Scientific and Technical Information (OSTI.GOV)
Burger, D.E.
1979-11-01
The extraction of morphological parameters from biological cells by analysis of light-scatter patterns is described. A light-scattering measurement system has been designed and constructed that allows one to visually examine and photographically record biological cells or cell models and measure the light-scatter pattern of an individual cell or cell model. Using a laser or conventional illumination, the imaging system consists of a modified microscope with a 35 mm camera attached to record the cell image or light-scatter pattern. Models of biological cells were fabricated. The dynamic range and angular distributions of light scattered from these models was compared to calculatedmore » distributions. Spectrum analysis techniques applied on the light-scatter data give the sought after morphological cell parameters. These results compared favorably to shape parameters of the fabricated cell models confirming the mathematical model procedure. For nucleated biological material, correct nuclear and cell eccentricity as well as the nuclear and cytoplasmic diameters were determined. A method for comparing the flow equivalent of nuclear and cytoplasmic size to the actual dimensions is shown. This light-scattering experiment provides baseline information for automated cytology. In its present application, it involves correlating average size as measured in flow cytology to the actual dimensions determined from this technique. (ERB)« less
Shiraya, Tomoyasu; Kato, Satoshi; Minami, Keiichiro; Miyata, Kazunori
2017-02-01
The aim of this study was to experimentally examine the changes in the transmittances of photocoagulation lasers when surface light scattering increases in AcrySof intraocular lenses (IOLs). SA60AT IOLs (Alcon) were acceleratingly aging for 0, 3, 5, and 10 years to simulate surface light scattering, and the surface light-scattering intensities of both IOL surfaces were measured using a Scheimpflug photographer. The powers of laser beams that passed from a laser photocoagulator through the aged IOLs were measured at 532, 577, and 647 nm. Changes in the laser power and transmittance with the years of aging and the intensities of surface light scattering were examined. Although the intensity of surface light scattering increased with the years of aging, the laser power did not change with the years of aging (P > 0.30, Kruskal-Wallis test). There were no significant changes in the laser transmittance with the years of aging or the laser wavelength (P > 0.30 and 0.57, respectively). The intensity of surface light scattering revealed no significant association with the laser transmittance at any wavelength (P > 0.37, liner regression). The increases in the surface light scattering of the AcrySof IOLs would not influence retinal photocoagulation treatments for up to 10 years after implantation.
Hughes, J; Clarke, F; Purslow, P; Warner, R
2018-05-18
Beef meat colour is impacted by both myoglobin status and the light scattering properties of the muscle, and the specific causative scattering elements of the latter are still unknown. We hypothesize that stretching muscles during rigor will generate a structure which favours light scattering, by increasing the length of the I-band (longer sarcomeres) and that a high rigor temperature will cause protein reconfiguration, changing the muscle structure and promoting light scattering. Muscle fibre fragments were isolated from four beef M. sternomandibularis and subjected to stretching (plus, minus) and three incubation temperatures (5, 15, 35 °C). Reflectance confocal laser scanning microscopy (rCLSM) revealed sarcomere stretching alone was not solely responsible for light scattering development. A high rigor temperature (35 °C) was more favourable for light scattering. Stretching and taking muscle into rigor at 35 °C promoted transverse shrinkage of muscle fibres and increased light scattering and could be applied post-mortem (PM) to reduce the occurrence of problematic dark meat. Crown Copyright © 2018. Published by Elsevier Ltd. All rights reserved.
Polarized light scattering as a probe for changes in chromosome structure
DOE Office of Scientific and Technical Information (OSTI.GOV)
Shapiro, Daniel Benjamin
1993-10-01
Measurements and calculations of polarized light scattering are applied to chromosomes. Calculations of the Mueller matrix, which completely describes how the polarization state of light is altered upon scattering, are developed for helical structures related to that of chromosomes. Measurements of the Mueller matrix are presented for octopus sperm heads, and dinoflagellates. Comparisons of theory and experiment are made. A working theory of polarized light scattering from helices is developed. The use of the first Born approximation vs the coupled dipole approximation are investigated. A comparison of continuous, calculated in this work, and discrete models is also discussed. By comparingmore » light scattering measurements with theoretical predictions the average orientation of DNA in an octopus sperm head is determined. Calculations are made for the Mueller matrix of DNA plectonemic helices at UV, visible and X-ray wavelengths. Finally evidence is presented that the chromosomes of dinoflagellates are responsible for observed differential scattering of circularly-polarized light. This differential scattering is found to vary in a manner that is possibly correlated to the cell cycle of the dinoflagellates. It is concluded that by properly choosing the wavelength probe polarized light scattering can provide a useful tool to study chromosome structure.« less
Ground-based full-sky imaging polarimeter based on liquid crystal variable retarders.
Zhang, Ying; Zhao, Huijie; Song, Ping; Shi, Shaoguang; Xu, Wujian; Liang, Xiao
2014-04-07
A ground-based full-sky imaging polarimeter based on liquid crystal variable retarders (LCVRs) is proposed in this paper. Our proposed method can be used to realize the rapid detection of the skylight polarization information with hemisphere field-of-view for the visual band. The characteristics of the incidence angle of light on the LCVR are investigated, based on the electrically controlled birefringence. Then, the imaging polarimeter with hemisphere field-of-view is designed. Furthermore, the polarization calibration method with the field-of-view multiplexing and piecewise linear fitting is proposed, based on the rotation symmetry of the polarimeter. The polarization calibration of the polarimeter is implemented with the hemisphere field-of-view. This imaging polarimeter is investigated by the experiment of detecting the skylight image. The consistency between the obtained experimental distribution of polarization angle with that due to Rayleigh scattering model is 90%, which confirms the effectivity of our proposed imaging polarimeter.
NASA Astrophysics Data System (ADS)
Huh, Jae-Won; Yu, Byeong-Hun; Shin, Dong-Myung; Yoon, Tae-Hoon
2015-03-01
Recently, a transparent display has got much attention as one of the next generation display devices. Especially, active studies on a transparent display using organic light-emitting diodes (OLEDs) are in progress. However, since it is not possible to obtain black color using a transparent OLED, it suffers from poor visibility. This inevitable problem can be solved by using a light shutter. Light shutter technology can be divided into two types; light absorption and scattering. However, a light shutter based on light absorption cannot block the background image perfectly and a light shutter based on light scattering cannot provide black color. In this work we demonstrate a light shutter using two liquid crystal (LC) layers, a light absorption layer and a light scattering layer. To realize a light absorption layer and a light scattering layer, we use the planar state of a dye-doped chiral nematic LC (CNLC) cell and the focal-conic state of a long-pitch CNLC cell, respectively. The proposed light shutter device can block the background image perfectly and show black color. We expect that the proposed light shutter can increase the visibility of a transparent display.
The HD 163296 Circumstellar Disk in Scattered Light: Evidence of Time-Variable Self-Shadowing
NASA Technical Reports Server (NTRS)
Wisniewski, John P.; Clampin, Mark; Grady, Carol A.; Ardila, David R.; Ford, Holland C.; Golimowski, David A.; Illingworth, Garth D.; Krist, John E.
2008-01-01
We present the first multi-color view of the scattered light disk of the Herbig Ae star HD 163296, based on coronagraphic observations from the Hubble Space Telescope Advanced Camera for Surveys (ACS). Radial profile fits of the surface brightness along the disk's semi-major axis indicates that the disk is not continuously flared, and extends to approx.540 AU. The disk's color (V-I)=1.1 at a radial distance of 3.5" is redder than the observed stellar color (V-I)=0.15. This red disk color might be indicative of either an evolution in the grain size distribution (i.e. grain growth) and/or composition, both of which would be consistent with the observed non-flared geometry of the outer disk. We also identify a single ansa morphological structure in our F435W ACS data, which is absent from earlier epoch F606W and F814W ACS data, but corresponds to one of the two ansa observed in archival HST STIS coronagraphic data. Following transformation to similar band-passes, we find that the scattered light disk of HD 163296 is 1 mag arcsec(sup -2) fainter at 3.5" in the STIS data than in the ACS data. Moreover, variations are seen in (i) the visibility of the ansa(e) structures, in (ii) the relative surface brightness of the ansa(e) structures, and in (iii) the (known) intrinsic polarization of the system. These results indicate that the scattered light from the HD 163296 disk is variable. We speculate that the inner disk wall, which Sitko et al. suggests has a variable scale height as diagnosed by near-IR SED variability, induces variable self-shadowing of the outer disk. We further speculate that the observed surface brightness variability of the ansa(e) structures may indicate that the inner disk wall is azimuthally asymmetric. Subject headings: circumstellar matter - stars: individual (HD 163296) - planetary systems: formation - planetary systems: protoplanetary disks
NASA Astrophysics Data System (ADS)
Bizheva, Kostadinka K.; Siegel, Andy M.; Boas, David A.
1998-12-01
We used low coherence interferometry to measure Brownian motion within highly scattering random media. A coherence gate was applied to resolve the optical path-length distribution and to separate ballistic from diffusive light. Our experimental analysis provides details on the transition from single scattering to light diffusion and its dependence on the system parameters. We found that the transition to the light diffusion regime occurs at shorter path lengths for media with higher scattering anisotropy or for larger numerical aperture of the focusing optics.
Laser light scattering from wood samples soaked in water or in benzyl benzoate
NASA Astrophysics Data System (ADS)
Simonaho, S.-P.; Tolonen, Y.; Rouvinen, J.; Silvennoinen, R.
Laser light scattering from Scots pine (Pinus Sylvesteris L.) wood samples soaked in two different liquids, which were tap water and benzyl benzoate, has been experimentally investigated. Differences in the characteristics of the scattering pattern as function of the soaking time as well as the moisture effect in the orientation of scattering pattern has been experimentally investigated. The wood samples soaked in the test liquids altered the laser light scattering in along and across the grain directions. No correlation between the content of the water in the wood sample and the orientation of laser light scattering pattern was observed.
Yaguchi, Shigeo; Nishihara, Hitoshi; Kambhiranond, Waraporn; Stanley, Daniel; Apple, David J
2008-01-01
To investigate the cause of light scatter measured on the surface of AcrySof intraocular lenses (Alcon Laboratories, Inc., Fort Worth, TX) retrieved from pseudophakic postmortem human eyes. Ten intraocular lenses (Alcon AcrySofModel MA60BM) were retrieved postmortem and analyzed for light scatter before and after removal of surface-bound biofilms. Six of the 10 lenses exhibited light scatter that was clearly above baseline levels. In these 6 lenses, both peak and average pixel density were reduced by approximately 80% after surface cleaning. The current study demonstrates that a coating deposited in vivo on the lens surface is responsible for the light scatter observed when incident light is applied.
NASA Astrophysics Data System (ADS)
Itoh, Harumi; Arai, Tsunenori; Kikuchi, Makoto
1997-06-01
We measured angular distribution of the light scattering from live mouse embryo with 632.8nm in wavelength to evaluate the embryo viability. We aim to measure the mitochondrial density in human embryo which have relation to the embryo viability. We have constructed the light scattering measurement system to detect the mitochondrial density non-invasively. We have employed two optical fibers for the illumination and sensing to change the angle between these fibers. There were two dips on the scattering angular distribution from the embryo. These dips existed on 30 and 85 deg. We calculated the scattering angular pattern by Mie theory to fit the measured scattering estimated scattering size and density. The best fitting was obtained when the particle size and density were 0.9 micrometers and 1010 particles per ml, respectively. These values coincided with the approximated values of mitochondrial in the embryo. The measured light scattering may mainly originated from mitochondria in spite of the existence of the various scattering particles in the embryo. Since our simple scattering measurement may offer the mitochondrial density in the embryo, it might become the practical method of human embryo on in vitro fertilization-embryo transfer.
Wang, Wenhao; Yu, Peng; Zhong, Zhiqin; Tong, Xin; Liu, Tianji; Li, Yanbo; Ashalley, Eric; Chen, Huanyang; Wu, Jiang; Wang, Zhiming
2018-08-31
Au nanobipyramids (NBPs) with sharp tips and narrow plasmon linewidths are ideal candidates for plasmonic applications. In this paper, we investigated the influencing factors of longitudinal plasmon resonance wavelength (LPRW) and scattering properties of single Au NBP by simulation. Compared with the volume, we establish the aspect ratio (length/width) as the dominant factor that affects the LPRW of Au NBPs. Plasmonic nanoparticles have been widely used for light-trapping enhancement in photovoltaics. To give a profound understanding of the superior light harvesting properties of Au NBPs, the near-field localization effect and far-field scattering mechanism of Au NBPs were investigated. Under the light injection at LPRW, the tip area shows near-field enhancement and the maximum scattering intensity appears on the side area of the waist owing to the remarkable optical absorption near the tips. Additionally, we confirm the fraction of light scattered into the substrate and angular distribution of the light scattered by the Au NBPs. The fraction of light scattered into the substrate reaches up to 97% from 400-1100 nm and preserves a broadband spectrum. This suggests that the NBP has a predominant forward scattering and reduced backward scattering. The excellent plasmonic scattering properties of Au NBPs are promising in photovoltaic devices and photothermal therapy.
NASA Astrophysics Data System (ADS)
Marciano, Tal; Peyrot, Donald; Crotti, Caroline; Alahyane, Fatima; Kowalczuk, Laura; Plamann, Karsten
2011-07-01
The optical properties of the cornea have been a research subject of great interest for many years. Several early theories have been put forward to explain with more or less success the optical transparency of this tissue, but it was not until Maurice demonstrated in a very elegant way during the 50s that this optical transparency could be explained by the regular ultrastructure of the cornea. When becoming edematous, the cornea's ultrastructure is perturbed and the tissue becomes a strongly scattering medium. With the emergence of ophthalmologic surgery by ultrashort pulse lasers in recent years, a regain of interest in the subject of corneal transparency arose. However, relatively little and no recent data of transparency spectra measurements covering a large wavelength range is available in the literature. The purpose of this study is to provide quantitative values for light scattering and its relation to the degree of edema by measuring the spectrum of transmitted light through corneas presenting different degrees of edema. This paper focus on the comparison of laboratory measurements published earlier with a new simple method we propose We also for eye banks to quantitatively measure the degree of transparency of corneal grafts by measuring the modulation transfer function of a Siemens star viewed through a corneal graft. Indeed, there is no current method to determine the transparency of corneal graft but the subjectivity of the laboratory technician or the ophthalmic surgeon.
Photovoltaic structures having a light scattering interface layer and methods of making the same
Liu, Xiangxin; Compaan, Alvin D.; Paudel, Naba Raj
2015-10-13
Photovoltaic (PV) cell structures having an integral light scattering interface layer configured to diffuse or scatter light prior to entering a semiconductor material and methods of making the same are described.
Yaguchi, Shigeo; Nishihara, Hitoshi; Kambhiranond, Waraporn; Stanley, Daniel; Apple, David
2008-01-01
To investigate the surface light scatter and optical quality of AcrySof lenses (Alcon Laboratories, Inc., Fort Worth, TX) following simulated aging of 20 years. AcrySof lenses were exposed to exaggerated thermal conditions to simulate up to 20 years of aging and were tested for surface light scatter and optical quality (modulation transfer function). There were no significant differences from baseline for either the surface light scatter or optical quality of the lenses over time. The current study demonstrated that surface light scatter on AcrySof lenses did not increase under conditions simulating 20 years of aging. Because the simulated aging environment contained no protein, this work indirectly supports the finding that surface light scatter is due to the deposition of a biomaterial on the lens surface rather than changes in the material. Optical performance integrity of the test lenses was maintained under severe environmental conditions.
Effective phase function of light scattered at small angles by polydisperse particulate media
NASA Astrophysics Data System (ADS)
Turcu, I.
2008-06-01
Particles with typical dimensions higher than the light wavelength and relative refraction indexes close to one, scatter light mainly in the forward direction where the scattered light intensity has a narrow peak. For particulate media accomplishing these requirements the light scattered at small angles in a far-field detecting set-up can be described analytically by an effective phase function (EPF) even in the multiple scattering regime. The EPF model which was built for monodispersed systems has been extended to polydispersed media. The main ingredients consist in the replacement of the single particle phase function and of the optical thickness with their corresponding averaged values. Using a Gamma particle size distribution (PSD) as a testing model, the effect of polydispersity was systematically investigated. The increase of the average radius or/and of the PSD standard deviation leads to the decrease of the angular spreading of the small angle scattered light.
Brillouin light scattering from surface acoustic waves in a subwavelength-diameter optical fibre
Beugnot, Jean-Charles; Lebrun, Sylvie; Pauliat, Gilles; Maillotte, Hervé; Laude, Vincent; Sylvestre, Thibaut
2014-01-01
Brillouin scattering in optical fibres is a fundamental interaction between light and sound with important implications ranging from optical sensors to slow and fast light. In usual optical fibres, light both excites and feels shear and longitudinal bulk elastic waves, giving rise to forward-guided acoustic wave Brillouin scattering and backward-stimulated Brillouin scattering. In a subwavelength-diameter optical fibre, the situation changes dramatically, as we here report with the first experimental observation of Brillouin light scattering from surface acoustic waves. These Rayleigh-type surface waves travel the wire surface at a specific velocity of 3,400 m s−1 and backscatter the light with a Doppler shift of about 6 GHz. As these acoustic resonances are sensitive to surface defects or features, surface acoustic wave Brillouin scattering opens new opportunities for various sensing applications, but also in other domains such as microwave photonics and nonlinear plasmonics. PMID:25341638
Earth Observation taken by the Expedition 29 crew
2011-10-15
ISS029-E-031157 (15 Oct. 2011) --- One of the Expedition 29 crew members aboard the International Space Station recorded this oblique view showing the Mediterranean Sea area, including the Nile River and the river's delta, and the Sinai Peninsula, on Oct. 15, 2011. Cyprus is visible at left. At first look, the image appears to have been photographed in daylight, but actually it was taken at 01:01:08 GMT. Some areas of the photo like the river and river delta appear as the brightest areas because of either man-made lighting (mostly incandescent) or man-made lighting reflected off nearby surfaces. The other areas appear to be illuminated naturally by moonlight, starlight, or back-scattered light from the atmosphere. A 20-mm focal length was used to record the image.
Earth Observation taken by the Expedition 29 crew
2011-10-15
ISS029-E-031143 (15 Oct. 2011) --- One of the Expedition 29 crew members aboard the International Space Station recorded this oblique view showing the Mediterranean Sea area, including parts of Turkey, the Nile River and the river's delta, and the Sinai Peninsula, on Oct. 15, 2011. At first look, the image appears to have been photographed in daylight, but actually it was taken at 01:01:26 GMT. Some areas of the photo like the river and river delta appear as the brightest areas because of either man-made lighting (mostly incandescent) or man-made lighting reflected off nearby surfaces. The other areas appear to be illuminated naturally by moonlight, starlight, or back-scattered light from the atmosphere. A 20-mm focal length was used to record the image.
Polarization simulations of stellar wind bow-shock nebulae - I. The case of electron scattering
NASA Astrophysics Data System (ADS)
Shrestha, Manisha; Neilson, Hilding R.; Hoffman, Jennifer L.; Ignace, Richard
2018-06-01
Bow shocks and related density enhancements produced by the winds of massive stars moving through the interstellar medium provide important information regarding the motions of the stars, the properties of their stellar winds, and the characteristics of the local medium. Since bow-shock nebulae are aspherical structures, light scattering within them produces a net polarization signal even if the region is spatially unresolved. Scattering opacity arising from free electrons and dust leads to a distribution of polarized intensity across the bow-shock structure. That polarization encodes information about the shape, composition, opacity, density, and ionization state of the material within the structure. In this paper, we use the Monte Carlo radiative transfer code SLIP to investigate the polarization created when photons scatter in a bow-shock-shaped region of enhanced density surrounding a stellar source. We present results for electron scattering, and investigate the polarization behaviour as a function of optical depth, temperature, and source of photons for two different cases: pure scattering and scattering with absorption. In both regimes, we consider resolved and unresolved cases. We discuss the implications of these results as well as their possible use along with observational data to constrain the properties of observed bow-shock systems. In different situations and under certain assumptions, our simulations can constrain viewing angle, optical depth and temperature of the scattering region, and the relative luminosities of the star and shock.
Method and apparatus for aerosol particle absorption spectroscopy
Campillo, Anthony J.; Lin, Horn-Bond
1983-11-15
A method and apparatus for determining the absorption spectra, and other properties, of aerosol particles. A heating beam source provides a beam of electromagnetic energy which is scanned through the region of the spectrum which is of interest. Particles exposed to the heating beam which have absorption bands within the band width of the heating beam absorb energy from the beam. The particles are also illuminated by light of a wave length such that the light is scattered by the particles. The absorption spectra of the particles can thus be determined from an analysis of the scattered light since the absorption of energy by the particles will affect the way the light is scattered. Preferably the heating beam is modulated to simplify the analysis of the scattered light. In one embodiment the heating beam is intensity modulated so that the scattered light will also be intensity modulated when the particles absorb energy. In another embodiment the heating beam passes through an interferometer and the scattered light reflects the Fourier Transform of the absorption spectra.
Evolution of circular and linear polarization in scattering environments
van der Laan, John D.; Wright, Jeremy Benjamin; Scrymgeour, David A.; ...
2015-12-02
This study quantifies the polarization persistence and memory of circularly polarized light in forward-scattering and isotropic (Rayleigh regime) environments; and for the first time, details the evolution of both circularly and linearly polarized states through scattering environments. Circularly polarized light persists through a larger number of scattering events longer than linearly polarized light for all forward-scattering environments; but not for scattering in the Rayleigh regime. Circular polarization’s increased persistence occurs for both forward and backscattered light. The simulated environments model polystyrene microspheres in water with particle diameters of 0.1 μm, 2.0 μm, and 3.0 μm. The evolution of the polarizationmore » states as they scatter throughout the various environments are illustrated on the Poincaré sphere after one, two, and ten scattering events.« less
A Study of Brownian Motion Using Light Scattering
ERIC Educational Resources Information Center
Clark, Noel A.; Lunacek, Joseph H.
1969-01-01
Describes an apparatus designed to investigate molecular motion by means of light scattering. Light from a He-Ne laser is focused into a cell containing a suspension of polystyrene spheres. The scattered light, collected on the photosurface of a photomultiplier tube, is analyzed. The apparatus won first prize in Demonstration Lecture Apparatus in…
Protein aggregation studied by forward light scattering and light transmission analysis
NASA Astrophysics Data System (ADS)
Penzkofer, A.; Shirdel, J.; Zirak, P.; Breitkreuz, H.; Wolf, E.
2007-12-01
The aggregation of the circadian blue-light photo-receptor cryptochrome from Drosophila melanogaster (dCry) is studied by transmission and forward light scattering measurement in the protein transparent wavelength region. The light scattering in forward direction is caused by Rayleigh scattering which is proportional to the degree of aggregation. The light transmission through the samples in the transparent region is reduced by Mie light scattering in all directions. It depends on the degree of aggregation and the monomer volume fill factor of the aggregates (less total scattering with decreasing monomer volume fill factor of protein globule) allowing a distinction between tightly packed protein aggregation (monomer volume fill factor 1) and loosely packed protein aggregation (monomer volume fill factor less than 1). An increase in aggregation with temperature, concentration, and blue-light exposure is observed. At a temperature of 4 °C and a protein concentration of less than 0.135 mM no dCry aggregation was observed, while at 24 °C and 0.327 mM gelation occurred (loosely packed aggregates occupying the whole solution volume).
Statistical-thermodynamic model for light scattering from eye lens protein mixtures
NASA Astrophysics Data System (ADS)
Bell, Michael M.; Ross, David S.; Bautista, Maurino P.; Shahmohamad, Hossein; Langner, Andreas; Hamilton, John F.; Lahnovych, Carrie N.; Thurston, George M.
2017-02-01
We model light-scattering cross sections of concentrated aqueous mixtures of the bovine eye lens proteins γB- and α-crystallin by adapting a statistical-thermodynamic model of mixtures of spheres with short-range attractions. The model reproduces measured static light scattering cross sections, or Rayleigh ratios, of γB-α mixtures from dilute concentrations where light scattering intensity depends on molecular weights and virial coefficients, to realistically high concentration protein mixtures like those of the lens. The model relates γB-γB and γB-α attraction strengths and the γB-α size ratio to the free energy curvatures that set light scattering efficiency in tandem with protein refractive index increments. The model includes (i) hard-sphere α-α interactions, which create short-range order and transparency at high protein concentrations, (ii) short-range attractive plus hard-core γ-γ interactions, which produce intense light scattering and liquid-liquid phase separation in aqueous γ-crystallin solutions, and (iii) short-range attractive plus hard-core γ-α interactions, which strongly influence highly non-additive light scattering and phase separation in concentrated γ-α mixtures. The model reveals a new lens transparency mechanism, that prominent equilibrium composition fluctuations can be perpendicular to the refractive index gradient. The model reproduces the concave-up dependence of the Rayleigh ratio on α/γ composition at high concentrations, its concave-down nature at intermediate concentrations, non-monotonic dependence of light scattering on γ-α attraction strength, and more intricate, temperature-dependent features. We analytically compute the mixed virial series for light scattering efficiency through third order for the sticky-sphere mixture, and find that the full model represents the available light scattering data at concentrations several times those where the second and third mixed virial contributions fail. The model indicates that increased γ-γ attraction can raise γ-α mixture light scattering far more than it does for solutions of γ-crystallin alone, and can produce marked turbidity tens of degrees celsius above liquid-liquid separation.
Light scattering from an atomic gas under conditions of quantum degeneracy
NASA Astrophysics Data System (ADS)
Porozova, V. M.; Gerasimov, L. V.; Havey, M. D.; Kupriyanov, D. V.
2018-05-01
Elastic light scattering from a macroscopic atomic sample existing in the Bose-Einstein condensate phase reveals a unique physical configuration of interacting light and matter waves. However, the joint coherent dynamics of the optical excitation induced by an incident photon is influenced by the presence of incoherent scattering channels. For a sample of sufficient length the excitation transports as a polariton wave and the propagation Green's function obeys the scattering equation which we derive. The polariton dynamics could be tracked in the outgoing channel of the scattered photon as we show via numerical solution of the scattering equation for one-dimensional geometry. The results are analyzed and compared with predictions of the conventional macroscopic Maxwell theory for light scattering from a nondegenerate atomic sample of the same density and size.
An analysis of scattered light in low dispersion IUE spectra
NASA Technical Reports Server (NTRS)
Basri, G.; Clarke, J. T.; Haisch, B. M.
1985-01-01
A detailed numerical simulation of light scattering from the low-resolution grating in the short wavelength spectrograph of the IUE Observatory was developed, in order to quantitatively analyze the effects of scattering on both continuum and line emission spectra. It is found that: (1) the redistribution of light by grating scattering did not appreciably alter either the shape or the absolute flux level of continuum spectra for A-F stars; (2) late-type stellar continua showed a tendency to flatten when observed in scattered light toward the shorter wavelengths; and (3) the effect of grating scattering on emission lines is to decrease measured line intensities by an increasing percentage toward the shorter wavelengths. The spectra obtained from scattering experiments for solar-type and late type stars are reproduced in graphic form.
The solar and heliospheric imager (SoloHI) instrument for the solar orbiter mission
NASA Astrophysics Data System (ADS)
Howard, Russell A.; Vourlidas, Angelos; Korendyke, Clarence M.; Plunkett, Simon P.; Carter, Michael T.; Wang, Dennis; Rich, Nathan; McMullin, Donald R.; Lynch, Sean; Thurn, Adam; Clifford, Greg; Socker, Dennis G.; Thernisien, Arnaud F.; Chua, Damien; Linton, Mark G.; Keller, David; Janesick, James R.; Tower, John; Grygon, Mark; Hagood, Robert; Bast, William; Liewer, Paulett C.; DeJong, Eric M.; Velli, Marco M. C.; Mikic, Zoran; Bothmer, Volker; Rochus, Pierre; Halain, Jean-Philippe; Lamy, Philippe L.
2013-09-01
The SoloHI instrument for the ESA/NASA Solar Orbiter mission will track density fluctuations in the inner heliosphere, by observing visible sunlight scattered by electrons in the solar wind. Fluctuations are associated with dynamic events such as coronal mass ejections, but also with the "quiescent" solar wind. SoloHI will provide the crucial link between the low corona observations from the Solar Orbiter instruments and the in-situ measurements on Solar Orbiter and the Solar Probe Plus missions. The instrument is a visible-light telescope, based on the SECCHI/Heliospheric Imager (HI) currently flying on the STEREO mission. In this concept, a series of baffles reduce the scattered light from the solar disk and reflections from the spacecraft to levels below the scene brightness, typically by a factor of 1012. The fluctuations are imposed against a much brighter signal produced by light scattered by dust particles (the zodiacal light/F-corona). Multiple images are obtained over a period of several minutes and are summed on-board to increase the signal-to-noise ratio and to reduce the telemetry load. SoloHI is a single telescope with a 40⁰ field of view beginning at 5° from the Sun center. Through a series of Venus gravity assists, the minimum perihelia for Solar Orbiter will be reduced to about 60 Rsun (0.28 AU), and the inclination of the orbital plane will be increased to a maximum of 35° after the 7 year mission. The CMOS/APS detector is a mosaic of four 2048 x 1930 pixel arrays, each 2-side buttable with 11 μm pixels.
Xu, Min; Wu, Tao T; Qu, Jianan Y
2008-01-01
A unified Mie and fractal model for light scattering by biological cells is presented. This model is shown to provide an excellent global agreement with the angular dependent elastic light scattering spectroscopy of cells over the whole visible range (400 to 700 nm) and at all scattering angles (1.1 to 165 deg) investigated. Mie scattering from the bare cell and the nucleus is found to dominate light scattering in the forward directions, whereas the random fluctuation of the background refractive index within the cell, behaving as a fractal random continuous medium, is found to dominate light scattering at other angles. Angularly dependent elastic light scattering spectroscopy aided by the unified Mie and fractal model is demonstrated to be an effective noninvasive approach to characterize biological cells and their internal structures. The acetowhitening effect induced by applying acetic acid on epithelial cells is investigated as an example. The changes in morphology and refractive index of epithelial cells, nuclei, and subcellular structures after the application of acetic acid are successfully probed and quantified using the proposed approach. The unified Mie and fractal model may serve as the foundation for optical detection of precancerous and cancerous changes in biological cells and tissues based on light scattering techniques.
Characterization of single particle aerosols by elastic light scattering at multiple wavelengths
NASA Astrophysics Data System (ADS)
Lane, P. A.; Hart, M. B.; Jain, V.; Tucker, J. E.; Eversole, J. D.
2018-03-01
We describe a system to characterize individual aerosol particles using stable and repeatable measurement of elastic light scattering. The method employs a linear electrodynamic quadrupole (LEQ) particle trap. Charged particles, continuously injected by electrospray into this system, are confined to move vertically along the stability line in the center of the LEQ past a point where they are optically interrogated. Light scattered in the near forward direction was measured at three different wavelengths using time-division multiplexed collinear laser beams. We validated our method by comparing measured silica microsphere data for four selected diameters (0.7, 1.0, 1.5 and 2.0 μm) to a model of collected scattered light intensities based upon Lorenz-Mie scattering theory. Scattered light measurements at the different wavelengths are correlated, allowing us to distinguish and classify inhomogeneous particles.
Optical Scatter Imaging with a digital micromirror device.
Zheng, Jing-Yi; Pasternack, Robert M; Boustany, Nada N
2009-10-26
We had developed Optical Scatter Imaging (OSI) as a method which combines light scattering spectroscopy with microscopic imaging to probe local particle size in situ. Using a variable diameter iris as a Fourier spatial filter, the technique consisted of collecting images that encoded the intensity ratio of wide-to-narrow angle scatter at each pixel in the full field of view. In this paper, we replace the variable diameter Fourier filter with a digital micromirror device (DMD) to extend our assessment of morphology to the characterization of particle shape and orientation. We describe our setup in detail and demonstrate how to eliminate aberrations associated with the placement of the DMD in a conjugate Fourier plane of our microscopic imaging system. Using bacteria and polystyrene spheres, we show how this system can be used to assess particle aspect ratio even when imaged at low resolution. We also show the feasibility of detecting alterations in organelle aspect ratio in situ within living cells. This improved OSI system could be further developed to automate morphological quantification and sorting of non-spherical particles in situ.
In vivo diagnosis of skin cancer using polarized and multiple scattered light spectroscopy
NASA Astrophysics Data System (ADS)
Bartlett, Matthew Allen
This thesis research presents the development of a non-invasive diagnostic technique for distinguishing between skin cancer, moles, and normal skin using polarized and multiple scattered light spectroscopy. Polarized light incident on the skin is single scattered by the epidermal layer and multiple scattered by the dermal layer. The epidermal light maintains its initial polarization while the light from the dermal layer becomes randomized and multiple scattered. Mie theory was used to model the epidermal light as the scattering from the intercellular organelles. The dermal signal was modeled as the diffusion of light through a localized semi-homogeneous volume. These models were confirmed using skin phantom experiments, studied with in vitro cell cultures, and applied to human skin for in vivo testing. A CCD-based spectroscopy system was developed to perform all these experiments. The probe and the theory were tested on skin phantoms of latex spheres on top of a solid phantom. We next extended our phantom study to include in vitro cells on top of the solid phantom. Optical fluorescent microscope images revealed at least four distinct scatterers including mitochondria, nucleoli, nuclei, and cell membranes. Single scattering measurements on the mammalian cells consistently produced PSD's in the size range of the mitochondria. The clinical portion of the study consisted of in vivo measurements on cancer, mole, and normal skin spots. The clinical study combined the single scattering model from the phantom and in vitro cell studies with the diffusion model for multiple scattered light. When parameters from both layers were combined, we found that a sensitivity of 100% and 77% can be obtained for detecting cancers and moles, respectively, given the number of lesions examined.
Imaging doppler lidar for wind turbine wake profiling
Bossert, David J.
2015-11-19
An imaging Doppler lidar (IDL) enables the measurement of the velocity distribution of a large volume, in parallel, and at high spatial resolution in the wake of a wind turbine. Because the IDL is non-scanning, it can be orders of magnitude faster than conventional coherent lidar approaches. Scattering can be obtained from naturally occurring aerosol particles. Furthermore, the wind velocity can be measured directly from Doppler shifts of the laser light, so the measurement can be accomplished at large standoff and at wide fields-of-view.
High-performance liquid chromatography analysis of plant saponins: An update 2005-2010
Negi, Jagmohan S.; Singh, Pramod; Pant, Geeta Joshi Nee; Rawat, M. S. M.
2011-01-01
Saponins are widely distributed in plant kingdom. In view of their wide range of biological activities and occurrence as complex mixtures, saponins have been purified and separated by high-performance liquid chromatography using reverse-phase columns at lower wavelength. Mostly, saponins are not detected by ultraviolet detector due to lack of chromophores. Electrospray ionization mass spectrometry, diode array detector , evaporative light scattering detection, and charged aerosols have been used for overcoming the detection problem of saponins. PMID:22303089
Polarization of Light from Leaves Measured from 0.5 - 1.6 mm
NASA Technical Reports Server (NTRS)
Vanderbilt, V. C.; Ustin, S. L.; Daughtry, C. S. T.; Walthal, C. L.; Greenberg, J. A.
2006-01-01
The light scattered by plant canopies depends in part on the light scattering/absorbing properties of the leaves. Insights into these properties gained at the leaf scale are necessary ultimately to accomplish the region and global scale environmental goals of the EOS era. While this scattered light may be described by the four components of the Stokes vector, (intensity, magnitude of line= polarization, angle of plane of linear polarization, and magnitude of circular polarization), significant progress has been achieved toward understanding only the first component, the intensity of the scattered light. Recent research shows that the magnitude of the linearly polarized light may be a significant part of the light scattered by some canopies. Thus, consideration of the second component may be necessary to obtain an unambiguous understanding of the canopy processes. We measured the intensity and the linear polarization of the light scattered by single leaves, testing the hypothesis that the polarization of the light scattered by each leaf was attributable to properties of the surfaces of the leaf and specifically did not depend upon the properties of the interior of the leaf. This research extends previous investigations limited to the single leaves of approximately 20 species typically found in the area of Lafayette, Indiana, to the leaves of 30 species representing monocots, dicots and ferns from six continents.
NASA Astrophysics Data System (ADS)
Xiong, Ming; Davies, Jackie A.; Li, Bo; Yang, Liping; Liu, Ying D.; Xia, Lidong; Harrison, Richard A.; Keiji, Hayashi; Li, Huichao
2017-07-01
Interplanetary corotating interaction regions (CIRs) can be remotely imaged in white light (WL), as demonstrated by the Solar Mass Ejection Imager (SMEI) on board the Coriolis spacecraft and Heliospheric Imagers (HIs) on board the twin Solar TErrestrial RElations Observatory (STEREO) spacecraft. The interplanetary WL intensity, due to Thomson scattering of incident sunlight by free electrons, is jointly determined by the 3D distribution of electron number density and line-of-sight (LOS) weighting factors of the Thomson-scattering geometry. The 2D radiance patterns of CIRs in WL sky maps look very different from different 3D viewpoints. Because of the in-ecliptic locations of both the STEREO and Coriolis spacecraft, the longitudinal dimension of interplanetary CIRs has, up to now, always been integrated in WL imagery. To synthesize the WL radiance patterns of CIRs from an out-of-ecliptic (OOE) vantage point, we perform forward magnetohydrodynamic modeling of the 3D inner heliosphere during Carrington Rotation CR1967 at solar maximum. The mixing effects associated with viewing 3D CIRs are significantly minimized from an OOE viewpoint. Our forward modeling results demonstrate that OOE WL imaging from a latitude greater than 60° can (1) enable the garden-hose spiral morphology of CIRs to be readily resolved, (2) enable multiple coexisting CIRs to be differentiated, and (3) enable the continuous tracing of any interplanetary CIR back toward its coronal source. In particular, an OOE view in WL can reveal where nascent CIRs are formed in the extended corona and how these CIRs develop in interplanetary space. Therefore, a panoramic view from a suite of wide-field WL imagers in a solar polar orbit would be invaluable in unambiguously resolving the large-scale longitudinal structure of CIRs in the 3D inner heliosphere.
NASA Astrophysics Data System (ADS)
Shapovalov, K. A.; Salmin, V. V.; Lazarenko, V. I.; Gar‧kavenko, V. V.
2017-05-01
The model of the autofluorescence spectrum formation of a crystalline lens taking into account light scattering was presented. Cross sections of extinction, scattering and absorption were obtained numerically for models of normal crystalline lens and cataract according to the Mie theory for polydisperse systems. To validate the model, data on the autofluorescence spectra of the normal lens and cataracts were obtained using an experimental ophthalmologic spectrofluorometer with excitation by UV light emitting diodes. In the framework of the model, the influence of the lens light scattering on the shape of the luminescence spectrum was estimated. It was found that the changes in the fluorescence spectrum of lenses with cataracts can be completely interpreted by the light scattering.
Till, Ugo; Gaucher-Delmas, Mireille; Saint-Aguet, Pascale; Hamon, Glenn; Marty, Jean-Daniel; Chassenieux, Christophe; Payré, Bruno; Goudounèche, Dominique; Mingotaud, Anne-Françoise; Violleau, Frédéric
2014-12-01
Polymersomes formed from amphiphilic block copolymers, such as poly(ethyleneoxide-b-ε-caprolactone) (PEO-b-PCL) or poly(ethyleneoxide-b-methylmethacrylate), were characterized by asymmetrical flow field-flow fractionation coupled with quasi-elastic light scattering (QELS), multi-angle light scattering (MALS), and refractive index detection, leading to the determination of their size, shape, and molecular weight. The method was cross-examined with more classical ones, like batch dynamic and static light scattering, electron microscopy, and atomic force microscopy. The results show good complementarities between all the techniques; asymmetrical flow field-flow fractionation being the most pertinent one when the sample exhibits several different types of population.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Noh, Heeso; Liew, Seng Fatt; Saranathan, Vinodkumar
2010-07-28
We measured the polarization- and angle-resolved optical scattering and reflection spectra of the quasiordered nanostructures in the bird feather barbs. In addition to the primary peak that originates from single scattering, we observed a secondary peak which exhibits depolarization and distinct angular dispersion. We explained the secondary peak in terms of double scattering, i.e., light is scattered successively twice by the structure. The two sequential single-scattering events are considered uncorrelated. Using the Fourier power spectra of the nanostructures obtained from the small-angle x-ray scattering experiment, we calculated the double scattering of light in various directions. The double-scattering spectrum is broadermore » than the single-scattering spectrum, and it splits into two subpeaks at larger scattering angle. The good agreement between the simulation results and the experimental data confirms that double scattering of light makes a significant contribution to the structural color.« less
Enhancing Localized Evaporation through Separated Light Absorbing Centers and Scattering Centers
Zhao, Dengwu; Duan, Haoze; Yu, Shengtao; Zhang, Yao; He, Jiaqing; Quan, Xiaojun; Tao, Peng; Shang, Wen; Wu, Jianbo; Song, Chengyi; Deng, Tao
2015-01-01
This report investigates the enhancement of localized evaporation via separated light absorbing particles (plasmonic absorbers) and scattering particles (polystyrene nanoparticles). Evaporation has been considered as one of the most important phase-change processes in modern industries. To improve the efficiency of evaporation, one of the most feasible methods is to localize heat at the top water layer rather than heating the bulk water. In this work, the mixture of purely light absorptive plasmonic nanostructures such as gold nanoparticles and purely scattering particles (polystyrene nanoparticles) are employed to confine the incident light at the top of the solution and convert light to heat. Different concentrations of both the light absorbing centers and the light scattering centers were evaluated and the evaporation performance can be largely enhanced with the balance between absorbing centers and scattering centers. The findings in this study not only provide a new way to improve evaporation efficiency in plasmonic particle-based solution, but also shed lights on the design of new solar-driven localized evaporation systems. PMID:26606898
Light scattering properties of self-organized nanostructured substrates for thin-film solar cells.
Mennucci, C; Del Sorbo, S; Pirotta, S; Galli, M; Andreani, L C; Martella, C; Giordano, M C; Buatier de Mongeot, F
2018-06-01
We investigate the scattering properties of novel kinds of nano-textured substrates, fabricated in a self-organized fashion by defocused ion beam sputtering. These substrates provide strong and broadband scattering of light and can be useful for applications in thin-film solar cells. In particular, we characterize the transmitted light in terms of haze and angle-resolved scattering, and we compare our results with those obtained for the commonly employed Asahi-U texture. The results indicate that the novel substrate has better scattering properties compared to reference Asahi-U substrates. We observe super-Lambertian light scattering behavior in selected spectral and angular regions due to the peculiar morphology of the nano-textured interface, which combines high aspect ratio pseudo random structures with a one-dimensional periodic pattern. The enhancement of light absorption observed in a prototype thin film semiconductor absorber grown on nano-textured glass with respect to an Asahi-U substrate further confirms the superior light trapping properties of the novel substrate.
NASA Technical Reports Server (NTRS)
Schaetzel, Klaus
1989-01-01
Since the development of laser light sources and fast digital electronics for signal processing, the classical discipline of light scattering on liquid systems experienced a strong revival plus an enormous expansion, mainly due to new dynamic light scattering techniques. While a large number of liquid systems can be investigated, ranging from pure liquids to multicomponent microemulsions, this review is largely restricted to applications on Brownian particles, typically in the submicron range. Static light scattering, the careful recording of the angular dependence of scattered light, is a valuable tool for the analysis of particle size and shape, or of their spatial ordering due to mutual interactions. Dynamic techniques, most notably photon correlation spectroscopy, give direct access to particle motion. This may be Brownian motion, which allows the determination of particle size, or some collective motion, e.g., electrophoresis, which yields particle mobility data. Suitable optical systems as well as the necessary data processing schemes are presented in some detail. Special attention is devoted to topics of current interest, like correlation over very large lag time ranges or multiple scattering.
Coherent Multiple Light Scattering in Ultracold Atomic Rb
NASA Astrophysics Data System (ADS)
Kulatunga, Pasad; Sukenik, C. I.; Balik, Salim; Havey, M. D.; Kupriyanov, D. V.; Sokolov, I. M.
2003-05-01
Wave transport in mesoscopic systems can be strongly influenced by coherent multiple scattering,which can lead to novel magneto-optic, transmission, and backscattering effects of light in atomic vapors. Although related to traditional studies of radiation trapping, in ultracold vapors negligible frequency or phase redistribution takes place in the scattering, and high-order coherent light scattering occurs. Among other things, this leads to enhancement of the influence of otherwise small non-resonant terms in the scattering amplitudes. We report investigation of multiple coherent light scattering from ultracold Rb atoms confined in a magneto-optic trap (MOT). In experimental studies, measurements are made of the angular, spectral, and polarization-dependent coherent backscattering profile of a low-intensity probe beam tuned near the F = 3 - F' = 4 hyperfine transition. The influence of higher probe beam intensity is also studied. In a theoretical study of angular intensity enhancement of backscattered light, we consider scattering orders up to 10 and a realistic and asymmetric Gaussian atom distribution in the MOT. Supported by NSF, NATO, and RFBR.
Light Scattering by Gaussian Particles: A Solution with Finite-Difference Time Domain Technique
NASA Technical Reports Server (NTRS)
Sun, W.; Nousiainen, T.; Fu, Q.; Loeb, N. G.; Videen, G.; Muinonen, K.
2003-01-01
The understanding of single-scattering properties of complex ice crystals has significance in atmospheric radiative transfer and remote-sensing applications. In this work, light scattering by irregularly shaped Gaussian ice crystals is studied with the finite-difference time-domain (FDTD) technique. For given sample particle shapes and size parameters in the resonance region, the scattering phase matrices and asymmetry factors are calculated. It is found that the deformation of the particle surface can significantly smooth the scattering phase functions and slightly reduce the asymmetry factors. The polarization properties of irregular ice crystals are also significantly different from those of spherical cloud particles. These FDTD results could provide a reference for approximate light-scattering models developed for irregular particle shapes and can have potential applications in developing a much simpler practical light scattering model for ice clouds angular-distribution models and for remote sensing of ice clouds and aerosols using polarized light. (copyright) 2003 Elsevier Science Ltd. All rights reserved.
Bidirectional scattering of light from tree leaves
NASA Technical Reports Server (NTRS)
Brakke, Thomas W.; Smith, James A.; Harnden, Joann M.
1989-01-01
A laboratory goniometer consisting of an He-Ne laser (632.8 nm), vertical leaf holder, and silicon photovoltaic detector was used to measure the bidirectional scattering (both transmittance and reflectance) of red oak and red maple. The illumination angles were 0, 30, and 60 deg, and the scattering was recorded approximately every 10 deg in the principal plane. The scattering profiles obtained show the non-Lambertian characteristics of the scattering, particularly for the off-nadir illumination directions. The transmitted light was more isotropic than the reflected light.
NASA Astrophysics Data System (ADS)
Shin, Jaewook; Bosworth, Bryan T.; Foster, Mark A.
2017-02-01
The process of multiple scattering has inherent characteristics that are attractive for high-speed imaging with high spatial resolution and a wide field-of-view. A coherent source passing through a multiple-scattering medium naturally generates speckle patterns with diffraction-limited features over an arbitrarily large field-of-view. In addition, the process of multiple scattering is deterministic allowing a given speckle pattern to be reliably reproduced with identical illumination conditions. Here, by exploiting wavelength dependent multiple scattering and compressed sensing, we develop a high-speed 2D time-stretch microscope. Highly chirped pulses from a 90-MHz mode-locked laser are sent through a 2D grating and a ground-glass diffuser to produce 2D speckle patterns that rapidly evolve with the instantaneous frequency of the chirped pulse. To image a scene, we first characterize the high-speed evolution of the generated speckle patterns. Subsequently we project the patterns onto the microscopic region of interest and collect the total light from the scene using a single high-speed photodetector. Thus the wavelength dependent speckle patterns serve as high-speed pseudorandom structured illumination of the scene. An image sequence is then recovered using the time-dependent signal received by the photodetector, the known speckle pattern evolution, and compressed sensing algorithms. Notably, the use of compressed sensing allows for reconstruction of a time-dependent scene using a highly sub-Nyquist number of measurements, which both increases the speed of the imager and reduces the amount of data that must be collected and stored. We will discuss our experimental demonstration of this approach and the theoretical limits on imaging speed.
Kinnunen, Matti; Kauppila, Antti; Karmenyan, Artashes; Myllylä, Risto
2011-09-15
Optical tweezers can be used to manipulate small objects and cells. A trap can be used to fix the position of a particle during light scattering measurements. The places of two separately trapped particles can also be changed. In this Letter we present elastic light scattering measurements as a function of scattering angle when two trapped spheres are illuminated with a He-Ne laser. This setup is suitable for trapping noncharged homogeneous spheres. We also demonstrate measurement of light scattering patterns from two separately trapped red blood cells. Two different illumination schemes are used for both samples.
Laser Rayleigh and Raman Diagnostics for Small Hydrogen/oxygen Rockets
NASA Technical Reports Server (NTRS)
Degroot, Wilhelmus A.; Zupanc, Frank J.
1993-01-01
Localized velocity, temperature, and species concentration measurements in rocket flow fields are needed to evaluate predictive computational fluid dynamics (CFD) codes and identify causes of poor rocket performance. Velocity, temperature, and total number density information have been successfully extracted from spectrally resolved Rayleigh scattering in the plume of small hydrogen/oxygen rockets. Light from a narrow band laser is scattered from the moving molecules with a Doppler shifted frequency. Two components of the velocity can be extracted by observing the scattered light from two directions. Thermal broadening of the scattered light provides a measure of the temperature, while the integrated scattering intensity is proportional to the number density. Spontaneous Raman scattering has been used to measure temperature and species concentration in similar plumes. Light from a dye laser is scattered by molecules in the rocket plume. Raman spectra scattered from major species are resolved by observing the inelastically scattered light with linear array mounted to a spectrometer. Temperature and oxygen concentrations have been extracted by fitting a model function to the measured Raman spectrum. Results of measurements on small rockets mounted inside a high altitude chamber using both diagnostic techniques are reported.
Stand-alone scattering optical device using holographic photopolymer (Conference Presentation)
NASA Astrophysics Data System (ADS)
Park, Jongchan; Lee, KyeoReh; Park, YongKeun
2016-03-01
When a light propagates through highly disordered medium, its optical parameters such as amplitude, phase and polarization states are completely scrambled because of multiple scattering events. Since the multiple scattering is a fundamental optical process that contains extremely high degrees of freedom, optical information of a transmitted light is totally mingled. Until recently, the presence of multiple scattering in an inhomogeneous medium is considered as a major obstacle when manipulating a light transmitting through the medium. However, a recent development of wavefront shaping techniques enable us to control the propagation of light through turbid media; a light transmitting through a turbid medium can be effectively controlled by modulating the spatial profile of the incident light using spatial light modulator. In this work, stand-alone scattering optical device is proposed; a holographic photopolymer film, which is much economic compared to the other digital spatial light modulators, is used to record and reconstruct permanent wavefront to generate optical field behind a scattering medium. By employing our method, arbitrary optical field can be generated since the scattering medium completely mixes all the optical parameters which allow us to access all the optical information only by modulating spatial phase profile of the impinging wavefront. The method is experimentally demonstrated in both the far-field and near-field regime where it shows promising fidelity and stability. The proposed stand-alone scattering optical device will opens up new avenues for exploiting the randomness inherent in disordered medium.
Airborne particle characterization by spatial scattering and fluorescence
NASA Astrophysics Data System (ADS)
Barton, John; Hirst, Edwin; Kaye, Paul; Saunders, Spencer; Clark, Don
1999-11-01
Several workers have reported the development of systems which allow the measurement of intrinsic fluorescence from particles irradiated with ultra-violet radiation. The fluorescence data are frequently recorded in conjunction with other parameters such as particle size, measured either as a function of optical scatter or as an aerodynamic size. The motivation for this work has been principally the detection of bioaerosols within an ambient environment. Previous work by the authors has shown that an analysis of the scattering profile of a particle, i.e.: the spatial distribution of light scattered by the particle carried in a sample air-stream, can provide an effective means of particle characterization and classification in terms of both size and shape parameters. Current work is aimed at the simultaneous recording of both spatial scattering and fluorescence data from individual particles with a view to substantially enhanced discrimination of biological aerosols. A prototype instrument has recently been completed which employs a cw 266 nm laser source to produce both elastic (spatial scattering) and inelastic (fluorescence) signals from individual airborne particles. The instrument incorporates a custom designed high-gain multi- pixel hybrid photodiode (HPD) to record the spatial scattering data and a single photomultiplier to record total fluorescence from the illuminated particle. Recorded data are processed to allow the classification of airborne particles on the basis of size, shape, and fluorescence for both biological and non- biological aerosols.
2015-09-14
The night sides of Saturn and Tethys are dark places indeed. We know that shadows are darker areas than sunlit areas, and in space, with no air to scatter the light, shadows can appear almost totally black. Tethys (660 miles or 1,062 kilometers across) is just barely seen in the lower left quadrant of this image below the ring plane and has been brightened by a factor of three to increase its visibility. The wavy outline of Saturn's polar hexagon is visible at top center. This view looks toward the sunlit side of the rings from about 10 degrees above the ring plane. The image was taken with the Cassini spacecraft wide-angle camera on Jan. 15, 2015 using a spectral filter which preferentially admits wavelengths of near-infrared light centered at 752 nanometers. The view was obtained at a distance of approximately 1.5 million miles (2.4 million kilometers) from Saturn. Image scale is 88 miles (141 kilometers) per pixel. http://photojournal.jpl.nasa.gov/catalog/PIA18333
NASA Astrophysics Data System (ADS)
Dolgos, G.; Martins, J.; Espinosa, R.; Dubovik, O.; Beyersdorf, A. J.; Ziemba, L. D.; Hair, J. W.
2013-12-01
Aerosols have a significant impact on the radiative balance and water cycle of our planet through influencing atmospheric radiation. Remote sensing of aerosols relies on scattering phase matrix information to retrieve aerosol properties with frequent global coverage, the assumed phase matrices must be validated by measurements. At the Laboratory for Aerosols, Clouds and Optics (LACO) at the University of Maryland, Baltimore County (UMBC) we developed a new technique to directly measure the aerosol phase function (P11), the degree of linear polarization of the scattered light (-P12/P11), and the volume scattering coefficient (SCAT). We designed and built a portable instrument called the Polarized Imaging Nephelometer (PI-Neph), shown in Figure 1 (a). The PI-Neph successfully participated in dozens of flights of the NASA Development and Evaluation of satellite ValidatiOn Tools by Experimenters (DEVOTE) project and the Deep Convective Clouds and Chemistry (DC3) project and the January and February deployment of the Deriving Information on Surface Conditions from Column and Vertically Resolved Observations Relevant to Air Quality (Discover-AQ) mission. The ambient aerosol enters the PI-Neph through an inlet and the sample is illuminated by laser light (wavelength of 532 nm); the scattered light is imaged by a stationary wide field of view camera in the scattering angle range of 2° to 178° (in some cases stray light limited the scattering angle range to 3° to 176°). Data for P11, P12, and SCAT were taken every 12 seconds, example datasets from DEVOTE of P11 times SCAT are shown on Figure 1 (b). The talk will highlight results from the three field deployments and will show microphysical retrievals from the scattering data. The size distribution and the average complex refractive index of the ambient aerosol ensemble can be retrieved from the data by an algorithm similar to that of AERONET, as illustrated in Figure 1 (c). Particle sphericity can potentially be retrieved as well, this will be investigated in the near future. The instrument will be applied to the validation of aerosol retrievals of AERONET and airborne polarimeters. The PI-Neph instrument has recently been upgraded to three wavelengths, and a second instrument was built as well. The LACO group is active in developing an advanced open path version of the Imaging Nephelometer that does not require an inlet but measures undisturbed particles under the aircraft wing. Figure 1. (a) The Polarized Imaging Nephelometer instrument inside the B200 aircraft of NASA Langley. (b) Phase function times volume scattering coefficient data from DEVOTE. (c) Retrievals of particle size distribution based on the data in panel (b).
NASA Technical Reports Server (NTRS)
Wells, Kelley C.; Martins, J. Vanderlei; Remer, Lorraine A.; Kreidenweis, Sonia M.; Stephens, Graeme L.
2012-01-01
Aerosols are tiny suspended particles in the atmosphere that scatter and absorb sunlight. Smoke particles are aerosols, as are sea salt, particulate pollution and airborne dust. When you look down at the earth from space sometimes you can see vast palls of whitish smoke or brownish dust being transported by winds. The reason that you can see these aerosols is because they are reflecting incoming sunlight back to the view in space. The reason for the difference in color between the different types of aerosol is that the particles arc also absorbing sunlight at different wavelengths. Dust appears brownish or reddish because it absorbs light in the blue wavelengths and scatters more reddish light to space, Knowing how much light is scattered versus how much is absorbed, and knowin that as a function of wavelength is essential to being able to quantify the role aerosols play in the energy balance of the earth and in climate change. It is not easy measuring the absorption properties of aerosols when they are suspended in the atmosphere. People have been doing this one substance at a time in the laboratory, but substances mix when they are in the atmosphere and the net absorption effect of all the particles in a column of air is a goal of remote sensing that has not yet been completely successful. In this paper we use a technique based on observing the point at which aerosols change from brightening the surface beneath to darkening it. If aerosols brighten a surface. they must scatter more light to space. If they darken the surface. they must be absorbing more. That cross over point is called the critical reflectance and in this paper we show that critical reflectance is a monotonic function of the intrinsic absorption properties of the particles. This parameter we call the single scattering albedo. We apply the technique to MODIS imagery over the Sahara and Sahel regions to retrieve the single scattering albedo in seven wavelengths, compare these retrievals to ground-based retrievals from AERONET instruments and compute error bars on each retrieval. The results show that we can retrieve single scattering albedo for pure dust to within +/-0.02 and mixtures of dust and smoke to within +/-0.03. No other space based instrument has achieved a retrieval of single scattering albedo that spans the spectrum from 0.47 microns to 2.13 microns and produces regional maps of aerosol absorption showing gradients and changes. Applied in a more operational fashion, such information will narrow uncertainties in estimating aerosol forcing on climate.
NASA Laser Light Scattering Advanced Technology Development Workshop, 1988
NASA Technical Reports Server (NTRS)
Meyer, William V. (Editor)
1989-01-01
The major objective of the workshop was to explore the capabilities of existing and prospective laser light scattering hardware and to assess user requirements and needs for a laser light scattering instrument in a reduced gravity environment. The workshop addressed experimental needs and stressed hardware development.
Utility of light scatter in the morphological analysis of sperm
We were able to differentiate the morphologically diverse sperm nuclei of four animal species by using an Ortho flow cytometer to detect the forward light scatter from a red (helium-neon) laser. Cytograms depicting the axial light loss and forward red scatter signals revealed uni...
Light Scattering by Marine Particles: Modeling with Non-spherical Shapes
2011-09-30
coccoliths detached from Emiliania huxleyi, Limnology and Oceanography, 46, 1438−1454, 2001. Gordon, H.R., T.J. Smyth, W.M. Balch, and G.C. Boynton...Light scattering by coccoliths detached from Emiliania huxleyi, Applied Optics, (2009). PUBLICATIONS H.R. Gordon, Light scattering by randomly
An Automated, High-Throughput System for GISAXS and GIWAXS Measurements of Thin Films
NASA Astrophysics Data System (ADS)
Schaible, Eric; Jimenez, Jessica; Church, Matthew; Lim, Eunhee; Stewart, Polite; Hexemer, Alexander
Grazing incidence small-angle X-ray scattering (GISAXS) and grazing incidence wide-angle X-ray scattering (GIWAXS) are important techniques for characterizing thin films. In order to meet rapidly increasing demand, the SAXSWAXS beamline at the Advanced Light Source (beamline 7.3.3) has implemented a fully automated, high-throughput system to conduct SAXS, GISAXS and GIWAXS measurements. An automated robot arm transfers samples from a holding tray to a measurement stage. Intelligent software aligns each sample in turn, and measures each according to user-defined specifications. Users mail in trays of samples on individually barcoded pucks, and can download and view their data remotely. Data will be pipelined to the NERSC supercomputing facility, and will be available to users via a web portal that facilitates highly parallelized analysis.
2000-01-20
Traveling west to east, the full moon, viewed from Merritt Island, Fla., at 10:35 p.m. EST, moves into the Earth's shadow during a lunar eclipse. Eclipses occur when the Sun, Earth and Moon line up. They are rare because the Moon usually passes above or below the imaginary line connecting Earth and the Sun. The Earth casts a shadow that the Moon can pass through -when it does, it is called a lunar eclipse. They can only occur when the moon is "full." During a total lunar eclipse the Moon takes on a dark red color because it is being lighted slightly by sunlight passing through the Earth's atmosphere and this light has the blue component preferentially scattered out (this is also why the sky appears blue from the surface of the Earth), leaving faint reddish light to illuminate the Moon during the eclipse
Dynamic Light Scattering Developed to Look Through the Eye's Window Into the Body
NASA Technical Reports Server (NTRS)
Stauber, Laurel J.
2001-01-01
Microgravity researcher Dr. Rafat R. Ansari, from the NASA Glenn Research Center, has found that the eye operates much like a camera and is the "window to the body." The eye contains transparent tissue through which light passes, providing us a view of what's going on inside. These transparent tissues represent nearly every tissue type that exists throughout the body. With the correlations and comparisons of these tissues done at Glenn, we hope to improve doctors' ability to diagnose diseases at much earlier stages. The medical community will be able to look noninvasively and quantitatively into a patient's eyes to detect disease before symptoms appear. Since the eye is easily accessed by light, the optical technologies created at Glenn can be used to evaluate its structure and physiology in health, aging, and disease.
Design and evaluation of an imaging spectrophotometer incorporating a uniform light source.
Noble, S D; Brown, R B; Crowe, T G
2012-03-01
Accounting for light that is diffusely scattered from a surface is one of the practical challenges in reflectance measurement. Integrating spheres are commonly used for this purpose in point measurements of reflectance and transmittance. This solution is not directly applicable to a spectral imaging application for which diffuse reflectance measurements are desired. In this paper, an imaging spectrophotometer design is presented that employs a uniform light source to provide diffuse illumination. This creates the inverse measurement geometry to the directional illumination/diffuse reflectance mode typically used for point measurements. The final system had a spectral range between 400 and 1000 nm with a 5.2 nm resolution, a field of view of approximately 0.5 m by 0.5 m, and millimeter spatial resolution. Testing results indicate illumination uniformity typically exceeding 95% and reflectance precision better than 1.7%.
The distribution of the scattered laser light in laser-plate-target coupling
NASA Astrophysics Data System (ADS)
Xiao-bo, Nie; Tie-qiang, Chang; Dong-xian, Lai; Shen-ye, Liu; Zhi-jian, Zheng
1997-04-01
Theoretical and experimental studies of the angular distributions of scattered laser light in laser-Au-plate-target coupling are reported. A simple model that describes three-dimensional plasmas and scattered laser light is presented. The approximate shape of critical density surface has been given and the three-dimensional laser ray tracing is applied in the model. The theoretical results of the model are consistent with the experimental data for the scattered laser light in the polar angle range of 25° to 145° from the laser beam.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Schneider, Glenn; Gaspar, Andras; Grady, Carol A.
We present new Hubble Space Telescope observations of three a priori known starlight-scattering circumstellar debris systems (CDSs) viewed at intermediate inclinations around nearby close-solar analog stars: HD 207129, HD 202628, and HD 202917. Each of these CDSs possesses ring-like components that are more massive analogs of our solar system's Edgeworth–Kuiper Belt. These systems were chosen for follow-up observations to provide imaging with higher fidelity and better sensitivity for the sparse sample of solar-analog CDSs that range over two decades in systemic ages, with HD 202628 and HD 207129 (both ∼2.3 Gyr) currently the oldest CDSs imaged in visible or near-IRmore » light. These deep (10–14 ks) observations, made with six-roll point-spread-function template visible-light coronagraphy using the Space Telescope Imaging Spectrograph, were designed to better reveal their angularly large debris rings of diffuse/low surface brightness, and for all targets probe their exo-ring environments for starlight-scattering materials that present observational challenges for current ground-based facilities and instruments. Contemporaneously also observing with a narrower occulter position, these observations additionally probe the CDS endo-ring environments that are seen to be relatively devoid of scatterers. We discuss the morphological, geometrical, and photometric properties of these CDSs also in the context of other CDSs hosted by FGK stars that we have previously imaged as a homogeneously observed ensemble. From this combined sample we report a general decay in quiescent-disk F {sub disk}/ F {sub star} optical brightness ∼ t {sup −0.8}, similar to what is seen at thermal IR wavelengths, and CDSs with a significant diversity in scattering phase asymmetries, and spatial distributions of their starlight-scattering grains.« less
Light scattering by magnons in whispering gallery mode cavities
NASA Astrophysics Data System (ADS)
Sharma, Sanchar; Blanter, Yaroslav M.; Bauer, Gerrit E. W.
2017-09-01
Brillouin light scattering is an established technique to study magnons, the elementary excitations of a magnet. Its efficiency can be enhanced by cavities that concentrate the light intensity. Here, we theoretically study inelastic scattering of photons by a magnetic sphere that supports optical whispering gallery modes in a plane normal to the magnetization. Magnons with low angular momenta scatter the light in the forward direction with a pronounced asymmetry in the Stokes and the anti-Stokes scattering strength, consistent with earlier studies. Magnons with large angular momenta constitute Damon-Eschbach modes which are shown to inelastically reflect light. The reflection spectrum contains either a Stokes or anti-Stokes peak, depending on the direction of the magnetization, a selection rule that can be explained by the chirality of the Damon-Eshbach magnons. The controllable energy transfer can be used to manage the thermodynamics of the magnet by light.
Soft bilateral filtering volumetric shadows using cube shadow maps
Ali, Hatam H.; Sunar, Mohd Shahrizal; Kolivand, Hoshang
2017-01-01
Volumetric shadows often increase the realism of rendered scenes in computer graphics. Typical volumetric shadows techniques do not provide a smooth transition effect in real-time with conservation on crispness of boundaries. This research presents a new technique for generating high quality volumetric shadows by sampling and interpolation. Contrary to conventional ray marching method, which requires extensive time, this proposed technique adopts downsampling in calculating ray marching. Furthermore, light scattering is computed in High Dynamic Range buffer to generate tone mapping. The bilateral interpolation is used along a view rays to smooth transition of volumetric shadows with respect to preserving-edges. In addition, this technique applied a cube shadow map to create multiple shadows. The contribution of this technique isreducing the number of sample points in evaluating light scattering and then introducing bilateral interpolation to improve volumetric shadows. This contribution is done by removing the inherent deficiencies significantly in shadow maps. This technique allows obtaining soft marvelous volumetric shadows, having a good performance and high quality, which show its potential for interactive applications. PMID:28632740
Nature of light scattering in dental enamel and dentin at visible and near-infrared wavelengths
NASA Astrophysics Data System (ADS)
Fried, Daniel; Glena, Richard E.; Featherstone, John D. B.; Seka, Wolf
1995-03-01
The light-scattering properties of dental enamel and dentin were measured at 543, 632, and 1053 nm. Angularly resolved scattering distributions for these materials were measured from 0 deg to 180 deg using a rotating goniometer. Surface scattering was minimized by immersing the samples in an index-matching bath. The scattering and absorption coefficients and the scattering phase function were deduced by comparing the measured scattering data with angularly resolved Monte Carlo light-scattering simulations. Enamel and dentin were best represented by a linear combination of a highly forward-peaked Henyey-Greenstein (HG) phase function and an isotropic phase function. Enamel weakly scatters light between 543 nm and 1.06 mu m, with the scattering coefficient ( mu s) ranging from mu s = 15 to 105 cm-1. The phase function is a combination of a HG function with g = 0.96 and a 30-60% isotropic phase function. For enamel, absorption is negligible. Dentin scatters strongly in the visible and near IR ( mu s approximately equals 260 cm-1) and absorbs weakly ( mu a approximately equals 4 cm-1). The scattering phase function for dentin is described by a HG function with g = 0.93 and a very weak isotropic scattering component ( approximately 2%).
Li, Feihu; Tang, Bingtao; Wu, Suli; Zhang, Shufen
2017-01-01
The synthesis and assembly of monodispersed colloidal spheres are currently the subject of extensive investigation to fabricate artificial structural color materials. However, artificial structural colors from general colloidal crystals still suffer from the low color visibility and strong viewing angle dependence which seriously hinder their practical application in paints, colorimetric sensors, and color displays. Herein, monodispersed polysulfide (PSF) spheres with intrinsic high refractive index (as high as 1.858) and light-absorbing characteristics are designed, synthesized through a facile polycondensation and crosslinking process between sodium disulfide and 1,2,3-trichloropropane. Owing to their high monodispersity, sufficient surface charge, and good dispersion stability, the PSF spheres can be assembled into large-scale and high-quality 3D photonic crystals. More importantly, high structural color visibility and broad viewing angle are easily achieved because the unique features of PSF can remarkably enhance the relative reflectivity and eliminate the disturbance of scattering and background light. The results of this study provide a simple and efficient strategy to create structural colors with high color visibility, which is very important for their practical application. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
NASA Astrophysics Data System (ADS)
Natraj, Vijay; Li, King-Fai; Yung, Yuk L.
2009-02-01
Tables that have been used as a reference for nearly 50 years for the intensity and polarization of reflected and transmitted light in Rayleigh scattering atmospheres have been found to be inaccurate, even to four decimal places. We convert the integral equations describing the X and Y functions into a pair of coupled integro-differential equations that can be efficiently solved numerically. Special care has been taken in evaluating Cauchy principal value integrals and their derivatives that appear in the solution of the Rayleigh scattering problem. The new approach gives results accurate to eight decimal places for the entire range of tabulation (optical thicknesses 0.02-1.0, surface reflectances 0-0.8, solar and viewing zenith angles 0°-88.85°, and relative azimuth angles 0°-180°), including the most difficult case of direct transmission in the direction of the sun. Revised tables have been created and stored electronically for easy reference by the planetary science and astrophysics community.
Plasma turbulence imaging using high-power laser Thomson scattering
NASA Astrophysics Data System (ADS)
Zweben, S. J.; Caird, J.; Davis, W.; Johnson, D. W.; Le Blanc, B. P.
2001-01-01
The two-dimensional (2D) structure of plasma density turbulence in a magnetically confined plasma can potentially be measured using a Thomson scattering system made from components of the Nova laser of Lawrence Livermore National Laboratory. For a plasma such as the National Spherical Torus Experiment at the Princeton Plasma Physics Laboratory, the laser would form an ≈10-cm-wide plane sheet beam passing vertically through the chamber across the magnetic field. The scattered light would be imaged by a charge coupled device camera viewing along the direction of the magnetic field. The laser energy required to make 2D images of density turbulence is in the range 1-3 kJ, which can potentially be obtained from a set of frequency-doubled Nd:glass amplifiers with diameters in the range of 208-315 mm. A laser pulse width of ⩽100 ns would be short enough to capture the highest frequency components of the expected density fluctuations.
Study of Light Scattering in the Human Eye
NASA Astrophysics Data System (ADS)
Perez, I. Kelly; Bruce, N. C.; Valdos, L. R. Berriel
2008-04-01
In this paper we present a numerical model of the human eye to be used in studies of the scattering of light in different components of the eye's optical system. Different parts of the eye are susceptible to produce scattering for different reasons; age, illness or injury. For example, cataracts can appear in the human lens or injuries or fungi can appear on the cornea. The aim of the study is to relate the backscattered light, which is what doctors measure or detect, to the forward scattered light, which is what affects the patient's vision. We present the model to be used, the raytrace procedure and some preliminary results for the image on the retina without scattering.
Weak scattering of scalar and electromagnetic random fields
NASA Astrophysics Data System (ADS)
Tong, Zhisong
This dissertation encompasses several studies relating to the theory of weak potential scattering of scalar and electromagnetic random, wide-sense statistically stationary fields from various types of deterministic or random linear media. The proposed theory is largely based on the first Born approximation for potential scattering and on the angular spectrum representation of fields. The main focus of the scalar counterpart of the theory is made on calculation of the second-order statistics of scattered light fields in cases when the scattering medium consists of several types of discrete particles with deterministic or random potentials. It is shown that the knowledge of the correlation properties for the particles of the same and different types, described with the newly introduced pair-scattering matrix, is crucial for determining the spectral and coherence states of the scattered radiation. The approach based on the pair-scattering matrix is then used for solving an inverse problem of determining the location of an "alien" particle within the scattering collection of "normal" particles, from several measurements of the spectral density of scattered light. Weak scalar scattering of light from a particulate medium in the presence of optical turbulence existing between the scattering centers is then approached using the combination of the Born's theory for treating the light interaction with discrete particles and the Rytov's theory for light propagation in extended turbulent medium. It is demonstrated how the statistics of scattered radiation depend on scattering potentials of particles and the power spectra of the refractive index fluctuations of turbulence. This theory is of utmost importance for applications involving atmospheric and oceanic light transmission. The second part of the dissertation includes the theoretical procedure developed for predicting the second-order statistics of the electromagnetic random fields, such as polarization and linear momentum, scattered from static media. The spatial distribution of these properties of scattered fields is shown to be substantially dependent on the correlation and polarization properties of incident fields and on the statistics of the refractive index distribution within the scatterers. Further, an example is considered which illustrates the usefulness of the electromagnetic scattering theory of random fields in the case when the scattering medium is a thin bio-tissue layer with the prescribed power spectrum of the refractive index fluctuations. The polarization state of the scattered light is shown to be influenced by correlation and polarization states of the illumination as well as by the particle size distribution of the tissue slice.
Faraday effect on stimulated Raman scattering in the linear region
NASA Astrophysics Data System (ADS)
Liu, Z. J.; Li, B.; Xiang, J.; Cao, L. H.; Zheng, C. Y.; Hao, L.
2018-04-01
The paper presents the effect of Faraday rotation on stimulated Raman scattering (SRS). When light propagates along the magnetic field upon plasma, Faraday rotation occurs. The rotation angle can be expressed as {{d}}θ /{{d}}{s}=2.93× {10}-4B\\tfrac{{n}e/{n}c}{\\sqrt{1-{n}e/{n}c}} {cm}}-1 approximately, where θ is the rotation angle and s is distance, n e is the electron density, n c is the critical density and B is magnetic field in unit of Gauss. Both the incident light and Raman light have Faraday effects. The angle between the polarization directions of incident light and Raman light changes with position. The driven force of electron plasma wave also reduces, and then SRS scattering level is reduced. Faraday rotation effect can increase the laser intensity threshold of Raman scattering, even if the magnetic field strength is small. The circularly polarized light incident case is also compared with that of the linearly polarized light incident. The Raman scattering level of linearly polarized light is much smaller than that of circularly polarized light in the magnetized plasma. The difference between linearly and circularly polarized lights is also discussed.
Polarization of Light by Leaves and Plant Canopies
NASA Technical Reports Server (NTRS)
Vanderbilt, V. C.
2006-01-01
This talk will focus first on the information contained in the surface-scattered light from leaves, plant canopies and surface waters. This light is in general polarized and depends upon surface roughness. Thus, for example, - The surface reflection from shiny green leaves measured in the specular direction shows no chlorophyll absorption bands, no 'red edge.' - Conversely, the degree of linear polarization of such light displays marked variation with wavelength having local maxima in the chlorophyll absorption bands and an inverted red edge. - Plant canopies with shiny leaves distributed in angle like the area on a sphere, specularly reflect sunlight in the subsolar or specular direction- but also in every other view direction. - Canopies of green plants may appear white not green when viewed obliquely toward the sun. - In a light to moderate wind, the often blindingly bright glitter of sunlight off smooth water surfaces provides a strong, angularly narrow signature reflection characteristic of inundated vegetated areas that are big sources of atmospheric methane, a climatically important greenhouse gas. (Conversely, a blindingly bright glitter-type reflection is uncharacteristic of upland or wind ruffled open water areas that are poor sources of atmospheric methane.) Because some of these results may be 'head scratchers,' it's always important to properly calibrate ones instruments. Indeed, as the second portion of the talk will show, the characteristics of the light measuring instrument, particularly its entrance aperture, may affect the results and should be taken into account during across-instrument data comparisons.
Plasmonic micropillars for precision cell force measurement across a large field-of-view
NASA Astrophysics Data System (ADS)
Xiao, Fan; Wen, Ximiao; Tan, Xing Haw Marvin; Chiou, Pei-Yu
2018-01-01
A plasmonic micropillar platform with self-organized gold nanospheres is reported for the precision cell traction force measurement across a large field-of-view (FOV). Gold nanospheres were implanted into the tips of polymer micropillars by annealing gold microdisks with nanosecond laser pulses. Each gold nanosphere is physically anchored in the center of a pillar tip and serves as a strong, point-source-like light scattering center for each micropillar. This allows a micropillar to be clearly observed and precisely tracked even under a low magnification objective lens for the concurrent and precision measurement across a large FOV. A spatial resolution of 30 nm for the pillar deflection measurement has been accomplished on this platform with a 20× objective lens.
System for diffusing light from an optical fiber or light guide
Maitland, Duncan J [Pleasant Hill, CA; Wilson, Thomas S [San Leandro, CA; Benett, William J [Livermore, CA; Small, IV, Ward [
2008-06-10
A system for diffusing light from an optical fiber wherein the optical fiber is coupled to a light source, comprising forming a polymer element adapted to be connected to the optical fiber and incorporating a scattering element with the polymer element wherein the scattering element diffuses the light from the polymer element. The apparatus of the present invention comprises a polymer element operatively connected to the optical fiber and a scattering element operatively connected with the shape polymer element that diffuses the light from the polymer element.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Guo, X. Z.; Shen, W. Z., E-mail: wzshen@sjtu.edu.cn; Laboratory of Condensed Matter Spectroscopy and Opto-Electronic Physics, and Key Laboratory of Artificial Structures and Quantum Control
2015-06-14
Composite photoanode comprising nanoparticles and one-dimensional (1D) nanostructure is a promising alternative to conventional photoanode for dye-sensitized solar cells (DSCs). Besides fast electron transport channels, the 1D nanostructure also plays as light scattering centers. Here, we theoretically investigate the light scattering properties of capsule-shaped 1D nanostructure and their influence on the light collection of DSCs. It is found that the far-field light scattering of a single capsule depends on its volume, shape, and orientation: capsules with bigger equivalent spherical diameter, smaller aspect ratio, and horizontal orientation demonstrate stronger light scattering especially at large scattering angle. Using Monte Carlo approach, wemore » simulated and optimized the light harvesting efficiency of the cell. Two multilayer composite photoanodes containing orderly or randomly oriented capsules are proposed. DSCs composed of these two photoanodes are promising for higher efficiencies because of their efficient light collection and superior electron collection. These results will provide practical guidance to the design and optimization of the photoanodes for DSCs.« less
Light scattering and random lasing in aqueous suspensions of hexagonal boron nitride nanoflakes
NASA Astrophysics Data System (ADS)
O'Brien, S. A.; Harvey, A.; Griffin, A.; Donnelly, T.; Mulcahy, D.; Coleman, J. N.; Donegan, J. F.; McCloskey, D.
2017-11-01
Liquid phase exfoliation allows large scale production of 2D materials in solution. The particles are highly anisotropic and strongly scatter light. While spherical particles can be accurately and precisely described by a single parameter—the radius, 2D nanoflakes, however, cannot be so easily described. We investigate light scattering in aqueous solutions of 2D hexagonal boron nitride nanoflakes in the single and multiple scattering regimes. In the single scattering regime, the anisotropic 2D materials show a much stronger depolarization of light when compared to spherical particles of similar size. In the multiple scattering regime, the scattering as a function of optical path for hexagonal boron nitride nanoflakes of a given lateral length was found to be qualitatively equivalent to scattering from spheres with the same diameter. We also report the presence of random lasing in high concentration suspensions of aqueous h-BN mixed with Rhodamine B dye. The h-BN works as a scattering agent and Rhodamine B as a gain medium for the process. We observed random lasing at 587 nm with a threshold energy of 0.8 mJ.
Light scattering and random lasing in aqueous suspensions of hexagonal boron nitride nanoflakes.
O'Brien, S A; Harvey, A; Griffin, A; Donnelly, T; Mulcahy, D; Coleman, J N; Donegan, J F; McCloskey, D
2017-11-24
Liquid phase exfoliation allows large scale production of 2D materials in solution. The particles are highly anisotropic and strongly scatter light. While spherical particles can be accurately and precisely described by a single parameter-the radius, 2D nanoflakes, however, cannot be so easily described. We investigate light scattering in aqueous solutions of 2D hexagonal boron nitride nanoflakes in the single and multiple scattering regimes. In the single scattering regime, the anisotropic 2D materials show a much stronger depolarization of light when compared to spherical particles of similar size. In the multiple scattering regime, the scattering as a function of optical path for hexagonal boron nitride nanoflakes of a given lateral length was found to be qualitatively equivalent to scattering from spheres with the same diameter. We also report the presence of random lasing in high concentration suspensions of aqueous h-BN mixed with Rhodamine B dye. The h-BN works as a scattering agent and Rhodamine B as a gain medium for the process. We observed random lasing at 587 nm with a threshold energy of 0.8 mJ.
Castaño-Martín, B; Gros-Otero, J; Martínez, J; Teus, M
2017-11-01
The purpose of this study was to determine the light scattering in patients with Fuchs' endothelial dystrophy without clinically significant corneal oedema, and evaluate its relationship with endothelial cell count, corneal thickness, and corneal biomechanical parameters. The values of light scattering were measured by C-Quant ® (Oculus Optikgeräte GmbH, Germany) in 32 eyes of 17 patients diagnosed with Fuchs' endothelial dystrophy without clinically significant corneal oedema. Corneal biomechanical properties were determined using ORA (ocular response) and Corvis ST ® (tonometry). A light scattering value outside the normal range was observed in 93.8% of eyes studied. No statistically significant association (P>.05) was found between the values of the measured light scattering by C-Quant ® and endothelial count, pachymetry, or corneal biomechanical properties. In this study, changes were found in the values of light scattering values of patients with corneal Fuchs' endothelial dystrophy. This change does not appear to correlate significantly with disease severity. Copyright © 2017 Sociedad Española de Oftalmología. Publicado por Elsevier España, S.L.U. All rights reserved.
POlarized Light Angle Reflectance Instrument I Polarized Incidence (POLAR:I)
NASA Technical Reports Server (NTRS)
Sarto, Anthony W.; Woldemar, Christopher M.; Vanderbilt, V. C.
1989-01-01
The light scattering properties of leaves are used as input data for models which mathematically describe the transport of photons within plant canopies. Polarization measurements may aid in the investigation of these properties. This paper describes an instrument for rapidly determining the bidirectional light scattering properties of leaves illuminated by linearly polarized light. Results for one species, magnolia, show large differences in the bidirectional light scattering properties depending whether or not the electric vector E is parallel to the foliage surface.
Micro-LiDAR velocity, temperature, density, concentration sensor
NASA Technical Reports Server (NTRS)
Dorrington, Adrian A. (Inventor); Danehy, Paul M. (Inventor)
2010-01-01
A light scatter sensor includes a sensor body in which are positioned a plurality of optical fibers. The sensor body includes a surface, in one end of each of the optical fibers terminates at the surface of the sensor body. One of the optical fibers is an illumination fiber for emitting light. A plurality of second optical fibers are collection fibers for collecting scattered light signals. A light sensor processor is connected to the collection fibers to detect the scattered light signals.
NASA Astrophysics Data System (ADS)
Abdelmonem, A.; Schnaiter, M.; Schön, R.; Leisner, T.
2009-04-01
Cirrus clouds impact climate by their influence on the water vapour distribution in the upper troposphere. Moreover, they directly affect the radiative balance of the Earth's atmosphere by the scattering of incoming solar radiation and the absorption of outgoing thermal emission. The link between the microphysical properties of ice cloud particles and the radiative forcing of the clouds is not as yet well understood and the influence of the shapes of ice crystals on the radiative budget of cirrus clouds is currently under debate. PHIPS is a new experimental device for the stereo-imaging of individual cloud particles and the simultaneous measurement of the polar scattering function of the same particle. PHIPS uses an automated particle event triggering system that ensures that only those particles are captured which are located in the field of view - depth of field volume of the microscope unit. Efforts were made to improve the resolution power of the microscope unit down to about 3 µm and to facilitate a 3D morphology impression of the ice crystals. This is realised by a stereo-imaging set up composed of two identical microscopes which image the same particle under an angular viewing distance of 30°. The scattering part of PHIPS enables the measurement of the polar light scattering function of cloud particles with an angular resolution of 1° for forward scattering directions (from 1° to 10°) and 8° for side and backscattering directions (from 18° to 170°). For each particle the light scattering pulse per channel is stored either as integrated intensity or as time resolved intensity function which opens a new category of data analysis concerning details of the particle movement. PHIPS is the first step to PHIPS-HALO which is one of the in situ ice particle and water vapour instruments that are currently under development for the new German research aircraft HALO. The instrument was tested in the ice cloud characterisation campaign HALO-02 which was conducted in December 2008 at the AIDA cloud chamber in the temperature range from -5°C to -70°C. In a series of experiments small externally generated seed ice crystals were grown in AIDA at distinct temperature and saturation ratio conditions. For these experiments the long known ice morphology diagram with the temperature dependent morphology changes and the supersaturation dependent structural complexity could clearly be reproduced by PHIPS. Structural details like hollow crystals, crystals with inclusions, and crystals with stepped surfaces (Hopper crystals) could be resolved by PHIPS. Moreover, the advantage of stereo-imaging in terms of habit classification and particle orientation deduction could be demonstrated. The scattering function measurement reveals ice particle orientation dependent specular reflection peaks which might contain information about the surface roughness. The presentation will describe the instrument set up in detail and highlight some preliminary results.
NASA Astrophysics Data System (ADS)
Kawauchi, Satoko; Sato, Shunichi; Ooigawa, Hidetoshi; Nawashiro, Hiroshi; Ishihara, Miya; Kikuchi, Makoto
2008-02-01
We performed simultaneous measurement of light scattering and absorption due to reduction of cytochrome c oxidase as intrinsic optical signals that are related to morphological characteristics and energy metabolism, respectively, for rat brains after oxygen/glucose deprivation by saline infusion. To detect change in light scattering, we determined the wavelength that was the most insensitive to change in light absorption due to the reduction of cytochrome c oxidase on the basis of multiwavelength analysis of diffuse reflectance data set for each rat. Then the relationships between scattering signal and absorption signals related to the reductions of heme aa 3 (605 nm) and CuA (830 nm) in cytochrome c oxidase were examined. Measurements showed that after starting saline infusion, the reduction of heme aa 3 started first; thereafter triphasic, large scattering change occurred (200-300 s), during which the reduction of CuA started. Despite such complex behaviors of IOSs, almost linear correlations were seen between the scattering signal and the heme aa 3-related absorption signal, while a relatively large animal-to-animal variation was observed in the correlation between the scattering signal and CuA-related absorption signal. Transmission electron microscopic observation revealed that dendritic swelling and mitochondrial deformation occurred in the cortical surface tissue after the triphasic scattering change. These results suggest that mitochondrial energy failure accompanies morphological alteration in the brain tissue and results in change in light scattering; light scattering will become an important indicator of tissue viability in brain.
Bright-White Beetle Scales Optimise Multiple Scattering of Light
NASA Astrophysics Data System (ADS)
Burresi, Matteo; Cortese, Lorenzo; Pattelli, Lorenzo; Kolle, Mathias; Vukusic, Peter; Wiersma, Diederik S.; Steiner, Ullrich; Vignolini, Silvia
2014-08-01
Whiteness arises from diffuse and broadband reflection of light typically achieved through optical scattering in randomly structured media. In contrast to structural colour due to coherent scattering, white appearance generally requires a relatively thick system comprising randomly positioned high refractive-index scattering centres. Here, we show that the exceptionally bright white appearance of Cyphochilus and Lepidiota stigma beetles arises from a remarkably optimised anisotropy of intra-scale chitin networks, which act as a dense scattering media. Using time-resolved measurements, we show that light propagating in the scales of the beetles undergoes pronounced multiple scattering that is associated with the lowest transport mean free path reported to date for low-refractive-index systems. Our light transport investigation unveil high level of optimisation that achieves high-brightness white in a thin low-mass-per-unit-area anisotropic disordered nanostructure.
Singh, M Suheshkumar; Yalavarthy, Phaneendra K; Vasu, R M; Rajan, K
2010-07-01
To assess the effect of ultrasound modulation of near infrared (NIR) light on the quantification of scattering coefficient in tissue-mimicking biological phantoms. A unique method to estimate the phase of the modulated NIR light making use of only time averaged intensity measurements using a charge coupled device camera is used in this investigation. These experimental measurements from tissue-mimicking biological phantoms are used to estimate the differential pathlength, in turn leading to estimation of optical scattering coefficient. A Monte-Carlo model based numerical estimation of phase in lieu of ultrasound modulation is performed to verify the experimental results. The results indicate that the ultrasound modulation of NIR light enhances the effective scattering coefficient. The observed effective scattering coefficient enhancement in tissue-mimicking viscoelastic phantoms increases with increasing ultrasound drive voltage. The same trend is noticed as the ultrasound modulation frequency approaches the natural vibration frequency of the phantom material. The contrast enhancement is less for the stiffer (larger storage modulus) tissue, mimicking tumor necrotic core, compared to the normal tissue. The ultrasound modulation of the insonified region leads to an increase in the effective number of scattering events experienced by NIR light, increasing the measured phase, causing the enhancement in the effective scattering coefficient. The ultrasound modulation of NIR light could provide better estimation of scattering coefficient. The observed local enhancement of the effective scattering coefficient, in the ultrasound focal region, is validated using both experimental measurements and Monte-Carlo simulations.
Scattering rings in optically anisotropic porous silicon
NASA Astrophysics Data System (ADS)
Oton, C. J.; Gaburro, Z.; Ghulinyan, M.; Pancheri, L.; Bettotti, P.; Negro, L. Dal; Pavesi, L.
2002-12-01
We report the observation of strongly anisotropic scattering of laser light at oblique incidence on a (100)-oriented porous silicon layer. The scattered light forms cones tangent to the incident and reflected beams. The conical pattern is caused by scattering on the vertical walls of pores, which are straight along the layer thickness. The light cone defines structured light rings onto a screen normal to the cone axis. We explain the various structures by optical anisotropy of porous silicon. For the sample under analysis, we directly measure from the ring patterns a value of Δn/nord=8% of positive birefringence.
Light propagation in dentin: influence of microstructure on anisotropy.
Kienle, Alwin; Forster, Florian K; Diebolder, Rolf; Hibst, Raimund
2003-01-21
We investigated the dependence of light propagation in human dentin on its microstructure. The main scatterers in dentin are the tubules, the shape of which can be approximated as long cylinders. We calculated the scattering of electromagnetic waves by an infinitely long cylinder and applied the results in a Monte Carlo code that simulates the light propagation in a dentin slab considering multi-scattering. The theory was compared with goniometric measurements. A pronounced anisotropic scattering pattern was found experimentally and theoretically. In addition, intensity peaks were measured which are shown to be caused by light diffraction by the tubules.
Light scattering from normal and cervical cancer cells.
Lin, Xiaogang; Wan, Nan; Weng, Lingdong; Zhou, Yong
2017-04-20
The light scattering characteristic plays a very important role in optic imaging and diagnostic applications. For optical detection of the cell, cell scattering characteristics have an extremely vital role. In this paper, we use the finite-difference time-domain (FDTD) algorithm to simulate the propagation and scattering of light in biological cells. The two-dimensional scattering cell models were set up based on the FDTD algorithm. The cell models of normal cells and cancerous cells were established, and the shapes of organelles, such as mitochondria, were elliptical. Based on these models, three aspects of the scattering characteristics were studied. First, the radar cross section (RCS) distribution curves of the corresponding cell models were calculated, then corresponding relationships between the size and the refractive index of the nucleus and light scattering information were analyzed in the three periods of cell canceration. The values of RCS increase positively with the increase of the nucleo-cytoplasmic ratio in the cancerous process when the scattering angle ranges from 0° to 20°. Second, the effect of organelles in the scattering was analyzed. The peak value of the RCS of cells with mitochondria is higher than the cells without mitochondria when the scattering angle ranges from 20° to 180°. Third, we demonstrated that the influence of cell shape is important, and the impact was revealed by the two typical ideal cells: round cells and oval cells. When the scattering angle ranges from 0° to 80°, the peak values and the frequencies of the appearance of the peaks from the two models are roughly similar. It can be concluded that: (1) the size of the nuclei and the change of the refractive index of cells have a certain impact on light scattering information of the whole cell; (2) mitochondria and other small organelles contribute to the cell light scattering characteristics in the larger scattering angle area; and (3) the change of the cell shape significantly influences the value of scattering peak and the deviation of scattering peak position. The results of the numerical simulation will guide subsequent experiments and early diagnosis of cervical cancer.
Fiber optic probe for light scattering measurements
Nave, Stanley E.; Livingston, Ronald R.; Prather, William S.
1995-01-01
A fiber optic probe and a method for using the probe for light scattering analyses of a sample. The probe includes a probe body with an inlet for admitting a sample into an interior sample chamber, a first optical fiber for transmitting light from a source into the chamber, and a second optical fiber for transmitting light to a detector such as a spectrophotometer. The interior surface of the probe carries a coating that substantially prevents non-scattered light from reaching the second fiber. The probe is placed in a region where the presence and concentration of an analyte of interest are to be detected, and a sample is admitted into the chamber. Exciting light is transmitted into the sample chamber by the first fiber, where the light interacts with the sample to produce Raman-scattered light. At least some of the Raman-scattered light is received by the second fiber and transmitted to the detector for analysis. Two Raman spectra are measured, at different pressures. The first spectrum is subtracted from the second to remove background effects, and the resulting sample Raman spectrum is compared to a set of stored library spectra to determine the presence and concentration of the analyte.
Fiber optic probe for light scattering measurements
Nave, S.E.; Livingston, R.R.; Prather, W.S.
1993-01-01
This invention is comprised of a fiber optic probe and a method for using the probe for light scattering analyses of a sample. The probe includes a probe body with an inlet for admitting a sample into an interior sample chamber, a first optical fiber for transmitting light from a source into the chamber, and a second optical fiber for transmitting light to a detector such as a spectrophotometer. The interior surface of the probe carries a coating that substantially prevents non-scattered light from reaching the second fiber. The probe is placed in a region where the presence and concentration of an analyte of interest are to be detected, and a sample is admitted into the chamber. Exciting light is transmitted into the sample chamber by the first fiber, where the light interacts with the sample to produce Raman-scattered light. At least some of the Raman- scattered light is received by the second fiber and transmitted to the detector for analysis. Two Raman spectra are measured, at different pressures. The first spectrum is subtracted from the second to remove background effects, and the resulting sample Raman spectrum is compared to a set of stored library spectra to determine the presence and concentration of the analyte.
NASA Technical Reports Server (NTRS)
2006-01-01
At least three different kinds of rocks await scientific analysis at the place where NASA's Mars Exploration Rover Spirit will likely spend several months of Martian winter. They are visible in this picture, which the panoramic camera on Spirit acquired during the rover's 809th sol, or Martian day, of exploring Mars (April 12, 2006). Paper-thin layers of light-toned, jagged-edged rocks protrude horizontally from beneath small sand drifts; a light gray rock with smooth, rounded edges sits atop the sand drifts; and several dark gray to black, angular rocks with vesicles (small holes) typical of hardened lava lie scattered across the sand. This view is an approximately true-color rendering that combines images taken through the panoramic camera's 753-nanometer, 535-nanometer, and 432-nanometer filters.Ultraviolet radiation cataract: dose dependence
NASA Astrophysics Data System (ADS)
Soderberg, Per G.; Loefgren, Stefan
1994-07-01
Current safety limits for cataract development after acute exposure to ultraviolet radiation (UVR) are based on experiments analyzing experimental data with a quantal, effect-no effect, dose-response model. The present study showed that intensity of forward light scattering is better described with a continuous dose-response model. It was found that 3, 30 and 300 kJ/m2UVR300nm induces increased light scattering within 6 h. For all three doses the intensity of forward light scattering was constant after 6 h. The intensity of forward light scattering was proportional to the log dose of UVR300nm. There was a slight increase of the intensity of forward light scattering on the contralateral side in animals that received 300 kJ/m2. Altogether 72 Sprague-Dawley male rats were included. Half of the rats were exposed in vivo on one side to UVR300nm. The other half was kept as a control group, receiving the same treatment as exposed rats but without delivery of UVR300nm to the eye. Subgroups of the rats received either of the three doses. Rats were sacrificed at varying intervals after the exposure. The lenses were extracted and the forward light scattering was estimated. It is concluded that intensity of forward light scattering in the lens after exposure to UVR300nm should be described with a continuous dose-reponse model.
Stray light analysis for the Thomson scattering diagnostic of the ETE Tokamak.
Berni, L A; Albuquerque, B F C
2010-12-01
Thomson scattering is a well-established diagnostic for measuring local electron temperature and density in fusion plasma, but this technique is particularly difficult to implement due to stray light that can easily mask the scattered signal from plasma. To mitigate this problem in the multipoint Thomson scattering system implemented at the ETE (Experimento Tokamak Esférico) a detailed stray light analysis was performed. The diagnostic system was simulated in ZEMAX software and scattering profiles of the mechanical parts were measured in the laboratory in order to have near realistic results. From simulation, it was possible to identify the main points that contribute to the stray signals and changes in the dump were implemented reducing the stray light signals up to 60 times.
NASA Astrophysics Data System (ADS)
Scheffold, Frank
2014-08-01
To characterize the structural and dynamic properties of soft materials and small particles, information on the relevant mesoscopic length scales is required. Such information is often obtained from traditional static and dynamic light scattering (SLS/DLS) experiments in the single scattering regime. In many dense systems, however, these powerful techniques frequently fail due to strong multiple scattering of light. Here I will discuss some experimental innovations that have emerged over the last decade. New methods such as 3D static and dynamic light scattering (3D LS) as well as diffusing wave spectroscopy (DWS) can cover a much extended range of experimental parameters ranging from dilute polymer solutions, colloidal suspensions to extremely opaque viscoelastic emulsions.
Kato, Haruhisa; Nakamura, Ayako; Takahashi, Kayori; Kinugasa, Shinichi
2012-01-01
Accurate determination of the intensity-average diameter of polystyrene latex (PS-latex) by dynamic light scattering (DLS) was carried out through extrapolation of both the concentration of PS-latex and the observed scattering angle. Intensity-average diameter and size distribution were reliably determined by asymmetric flow field flow fractionation (AFFFF) using multi-angle light scattering (MALS) with consideration of band broadening in AFFFF separation. The intensity-average diameter determined by DLS and AFFFF-MALS agreed well within the estimated uncertainties, although the size distribution of PS-latex determined by DLS was less reliable in comparison with that determined by AFFFF-MALS. PMID:28348293
Literature survey for suppression of scattered light in large space telescopes
NASA Technical Reports Server (NTRS)
Tifft, W. G.; Fannin, B. B.
1973-01-01
A literature survey is presented of articles dealing with all aspects of predicting, measuring, and controlling unwanted scattered (stray) light. The survey is divided into four broad classifications: (1) existing baffle/telescope designs; (2) computer programs for the analysis/design of light suppression systems; (3) the mechanism, measurement, and control of light scattering; and (4) the advantages and problems introduced by the space environment for the operation of diffraction-limited optical systems.
Improving human object recognition performance using video enhancement techniques
NASA Astrophysics Data System (ADS)
Whitman, Lucy S.; Lewis, Colin; Oakley, John P.
2004-12-01
Atmospheric scattering causes significant degradation in the quality of video images, particularly when imaging over long distances. The principle problem is the reduction in contrast due to scattered light. It is known that when the scattering particles are not too large compared with the imaging wavelength (i.e. Mie scattering) then high spatial resolution information may be contained within a low-contrast image. Unfortunately this information is not easily perceived by a human observer, particularly when using a standard video monitor. A secondary problem is the difficulty of achieving a sharp focus since automatic focus techniques tend to fail in such conditions. Recently several commercial colour video processing systems have become available. These systems use various techniques to improve image quality in low contrast conditions whilst retaining colour content. These systems produce improvements in subjective image quality in some situations, particularly in conditions of haze and light fog. There is also some evidence that video enhancement leads to improved ATR performance when used as a pre-processing stage. Psychological literature indicates that low contrast levels generally lead to a reduction in the performance of human observers in carrying out simple visual tasks. The aim of this paper is to present the results of an empirical study on object recognition in adverse viewing conditions. The chosen visual task was vehicle number plate recognition at long ranges (500 m and beyond). Two different commercial video enhancement systems are evaluated using the same protocol. The results show an increase in effective range with some differences between the different enhancement systems.
First Scattered-Light Images of the Gas-Rich Debris Disk Around 49 Ceti
NASA Technical Reports Server (NTRS)
Choquet, Elodie; Milli, Julien; Wahhaj, Zahed; Soummer, Remi; Roberge, Aki; Augereau, Jean-Charles; Booth, Mark; Absil, Olivier; Boccaletti, Anthony; Chen, Christine H.;
2017-01-01
We present the first scattered-light images of the debris disk around 49 Ceti, a approximately 40 Myr A1 main-sequence star at 59 pc, famous for hosting two massive dust belts as well as large quantities of atomic and molecular gas. The outer disk is revealed in reprocessed archival Hubble Space Telescope NICMOS-F110W images, as well as new coronagraphic H-band images from the Very Large Telescope SPHERE instrument. The disk extends from 1."1 (65 au) to 4." 6 (250 au) and is seen at an inclination of 73 deg, which refines previous measurements at lower angular resolution. We also report no companion detection larger than 3 MJup at projected separations beyond 20 au from the star (0." 34). Comparison between the F110W and H-band images is consistent with a gray color of 49 Ceti's dust, indicating grains larger than approximately greater than 2 micrometers. Our photometric measurements indicate a scattering efficiency/infrared excess ratio of 0.2-0.4, relatively low compared to other characterized debris disks. We find that 49 Ceti presents morphological and scattering properties very similar to the gas-rich HD 131835 system. From our constraint on the disk inclination we find that the atomic gas previously detected in absorption must extend to the inner disk, and that the latter must be depleted of CO gas. Building on previous studies, we propose a schematic view of the system describing the dust and gas structure around 49 Ceti and hypothetical scenarios for the gas nature and origin.
Experimental observation of multiphoton Thomson scattering
NASA Astrophysics Data System (ADS)
Yan, Wenchao; Golovin, Grigory; Fruhling, Colton; Haden, Daniel; Zhang, Ping; Zhang, Jun; Zhao, Baozhen; Liu, Cheng; Chen, Shouyuan; Banerjee, Sudeep; Umstadter, Donald
2016-10-01
With the advent of high-power lasers, several multiphoton processes have been reported involving electrons in strong fields. For electrons that were initially bound to atoms, both multiphoton ionization and scattering have been reported. However, for free electrons, only low-order harmonic generation has been observed until now. This limitation stems from past difficulty in achieving the required ultra-high-field strengths in scattering experiments. Highly relativistic laser intensities are required to reach the multiphoton regime of Thomson scattering, and generate high harmonics from free electrons. The scaling parameter is the normalized vector potential (a0). Previous experiments have observed phenomena in the weakly relativistic case (a0 >> 1). In ultra-intense fields (a0 >>1), the anomalous electron trajectory is predicted to produce a spectrum characterized by the merging of multiple high-order harmonic generation into a continuum. This may be viewed as the multiphoton Thomson scattering regime analogous to the wiggler of a synchrotron. Thus, the light produced reflects the electrons behavior in an ultra-intense lase field. We discuss the first experiments in the highly relativistic case (a0 15). This material is based upon work supported by NSF No. PHY-153700; US DOE, Office of Science, BES, # DE-FG02-05ER15663; AFOSR # FA9550-11-1-0157; and DHS DNDO # HSHQDC-13-C-B0036.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Xia, Weiwei; Mei, Chao; Zeng, Xianghua, E-mail: xhzeng@yzu.edu.cn
2016-03-14
Both light scattering and dye adsorbing are important for the power conversion efficiency PCE performance of dye sensitized solar cell (DSSC). Nanostructured scattering layers with a large specific surface area are regarded as an efficient way to improve the PCE by increasing dye adsorbing, but excess adsorbed dye will hinder light scattering and light penetration. Thus, how to balance the dye adsorbing and light penetration is a key problem to improve the PCE performance. Here, multiple-shelled ZnO microspheres with a mesoporous surface are fabricated by a hydrothermal method and are used as scattering layers on the TiO{sub 2} photoanode ofmore » the DSSC in the presence of N719 dye and iodine–based electrolyte, and the results reveal that the DSSCs based on triple shelled ZnO microsphere with a mesoporous surface exhibit an enhanced PCE of 7.66%, which is 13.0% higher than those without the scattering layers (6.78%), indicating that multiple-shelled microspheres with a mesoporous surface can ensure enough light scattering between the shells, and a favorable concentration of the adsorbed dye can improve the light penetration. These results may provide a promising pathway to obtain the high efficient DSSCs.« less
Analysis of the scattering performance of human retinal tissue layers
NASA Astrophysics Data System (ADS)
Zhu, Dan; Gao, Zhisan; Ye, Haishui; Yuan, Qun
2017-02-01
Human retina is different from other ocular tissues, such as cornea, crystalline lens and vitreous because of high scattering performance. As an anisotropic tissue, we cannot neglect its impact on the polarization state of the scattered light. In this paper, Mie scattering and radiative transfer theory are applied to analyze the polarization state of backscattered light from four types of retinal tissues, including neural retina, retinal pigment epithelial (RPE), choroid and sclera. The results show that the most backscattered zones in different depths have almost the same electrical fields of Jones vector, which represents the polarization state of light, whether neural retina layer is under normal incidence or oblique incidence. Very little change occurs in the polarization of backscattered light compared to that of the incident light. Polarization distribution of backward scattered light from neural retina layer doesn't make apparent effects on polarization phase shifting in spectral domain OCT because its thickness is far less than photon mean free path, while other retinal tissues do not meet this rule.
Study of resonance light scattering for remote optical probing
NASA Technical Reports Server (NTRS)
Penney, C. M.; Morey, W. W.; St. Peters, R. L.; Silverstein, S. D.; Lapp, M.; White, D. R.
1973-01-01
Enhanced scattering and fluorescence processes in the visible and UV were investigated which will enable improved remote measurements of gas properties. The theoretical relationship between scattering and fluorescence from an isolated molecule in the approach to resonance is examined through analysis of the time dependence of re-emitted light following excitation of pulsed incident light. Quantitative estimates are developed for the relative and absolute intensities of fluorescence and resonance scattering. New results are obtained for depolarization of scattering excited by light at wavelengths within a dissociative continuum. The experimental work was performed in two separate facilities. One of these utilizes argon and krypton lasers, single moded by a tilted etalon, and a 3/4 meter double monochromator. This facility was used to determine properties of the re-emission from NO2, I2 and O3 excited by visible light. The second facility involves a narrow-line dye laser, and a 3/4 meter single monochromator. The dye laser produces pulsed light with 5 nsec pulse duration and 0.005 nm spectral width.
FDTD scattered field formulation for scatterers in stratified dispersive media.
Olkkonen, Juuso
2010-03-01
We introduce a simple scattered field (SF) technique that enables finite difference time domain (FDTD) modeling of light scattering from dispersive objects residing in stratified dispersive media. The introduced SF technique is verified against the total field scattered field (TFSF) technique. As an application example, we study surface plasmon polariton enhanced light transmission through a 100 nm wide slit in a silver film.
Process for sensing defects on a smooth cylindrical interior surface in tubing
Dutton, G. Wayne
1987-11-17
The cylindrical interior surface of small diameter metal tubing is optically inspected to determine surface roughness by passing a slightly divergent light beam to illuminate the entire interior surface of the tubing. Impingement of the input light beam components on any rough spots on the interior surface generates forward and backward scattered radiation components. The forward scattered components can be measured by blocking direct and specular radiation components exiting the tubing while allowing the forward scattered radiation to travel past the blocking location. Collecting optics are employed to converge the forward scattered radiation onto a photodetector generating a signal indicative of surface roughness. In the back scattered mode, back scattered radiation exiting the tubing through the entrance opening is reflected 90.degree. by a beam splitter towards collecting optics and a photodetector. Alternatively, back scattered radiation can be transmitted through a fiber optic bundle towards the collecting optics. The input light beam can be supplied through a white light fiber optic bundle mounted coaxial with the first bundle.
Process for sensing defects on a smooth cylindrical interior surface in tubing
Dutton, G.W.
1987-11-17
The cylindrical interior surface of small diameter metal tubing is optically inspected to determine surface roughness by passing a slightly divergent light beam to illuminate the entire interior surface of the tubing. Impingement of the input light beam components on any rough spots on the interior surface generates forward and backward scattered radiation components. The forward scattered components can be measured by blocking direct and specular radiation components exiting the tubing while allowing the forward scattered radiation to travel past the blocking location. Collecting optics are employed to converge the forward scattered radiation onto a photodetector generating a signal indicative of surface roughness. In the back scattered mode, back scattered radiation exiting the tubing through the entrance opening is reflected 90[degree] by a beam splitter towards collecting optics and a photodetector. Alternatively, back scattered radiation can be transmitted through a fiber optic bundle towards the collecting optics. The input light beam can be supplied through a white light fiber optic bundle mounted coaxial with the first bundle. 6 figs.
Process and apparatus for sensing defects on a smooth cylindrical surface in tubing
Dutton, G.W.
1985-08-05
The cylindrical interior surface of small diameter metal tubing is optically inspected to determine surface roughness by passing a slightly divergent light beam to illuminate the entire interior surface of the tubing. Impingement of the input light beam components on any rough spots on the interior surface generates forward and backward scattered radiation components. The forward scattered components can be measured by blocking direct and specular radiation components exiting the tubing while allowing the forward scattered radiation to travel past the blocking location. Collecting optics are employed to converge the forward scattered radiation onto a photodetector generating a signal indicative of surface roughness. In the back scattered mode, back scattered radiation exiting the tubing through the entrance opening is reflected 90/sup 0/ by a beam splitter towards collecting optics and a photodetector. Alternatively, back scattered radiation can be transmitted through a fiber optic bundle towards the collecting optics. The input light beam can be supplied through a white light fiber optic bundle mounted coaxial with the first bundle.
Light scattering properties of kidney epithelial cells and nuclei
NASA Astrophysics Data System (ADS)
Vitol, Elina A.; Kurzweg, Timothy P.; Nabet, Bahram
2006-02-01
Enlargement of mammalian cells nuclei due to the cancerous inflammation can be detected early through noninvasive optical techniques. We report on the results of cellular experiments, aimed towards the development of a fiber optic endoscopic probe used for precancerous detection of Barrett's esophagus. We previously presented white light scattering results from tissue phantoms (polystyrene polybead microspheres). In this paper, we discuss light scattering properties of epithelial MDCK (Madine-Darby Canine Kidney) cells and cell nuclei suspensions. A bifurcated optical fiber is used for experimental illumination and signal detection. The resulting scattering spectra from the cells do not exhibit the predicted Mie theory oscillatory behavior inherent to ideally spherical scatterers, such as polystyrene microspheres. However, we are able to demonstrate that the Fourier transform spectra of the cell suspensions are well correlated with the Fourier transform spectra of cell nuclei, concluding that the dominate scatterer in the backscattering region is the nucleus. This correlation experimentally illustrates that in the backscattering region, the cell nuclei are the main scatterer in the cells of the incident light.
Determination of wood grain direction from laser light scattering pattern
NASA Astrophysics Data System (ADS)
Simonaho, Simo-Pekka; Palviainen, Jari; Tolonen, Yrjö; Silvennoinen, Raimo
2004-01-01
Laser light scattering patterns from the grains of wood are investigated in detail to gain information about the characteristics of scattering patterns related to the direction of the grains. For this purpose, wood samples of Scots pine ( Pinus sylvestris L.) and silver birch ( Betula pubescens) were investigated. The orientation and shape of the scattering pattern of laser light in wood was found to correlate well with the direction of grain angles in a three-dimensional domain. The proposed method was also experimentally verified.
Sopori, Bhushan L.
1995-01-01
Apparatus for detecting and mapping defects in the surfaces of polycrystalline materials in a manner that distinguishes dislocation pits from grain boundaries includes a laser for illuminating a wide spot on the surface of the material, a light integrating sphere with apertures for capturing light scattered by etched dislocation pits in an intermediate range away from specular reflection while allowing light scattered by etched grain boundaries in a near range from specular reflection to pass through, and optical detection devices for detecting and measuring intensities of the respective intermediate scattered light and near specular scattered light. A center blocking aperture or filter can be used to screen out specular reflected light, which would be reflected by nondefect portions of the polycrystalline material surface. An X-Y translation stage for mounting the polycrystalline material and signal processing and computer equipment accommodate rastor mapping, recording, and displaying of respective dislocation and grain boundary defect densities. A special etch procedure is included, which prepares the polycrystalline material surface to produce distinguishable intermediate and near specular light scattering in patterns that have statistical relevance to the dislocation and grain boundary defect densities.
Sopori, B.L.
1995-04-11
Apparatus for detecting and mapping defects in the surfaces of polycrystalline materials in a manner that distinguishes dislocation pits from grain boundaries includes a laser for illuminating a wide spot on the surface of the material, a light integrating sphere with apertures for capturing light scattered by etched dislocation pits in an intermediate range away from specular reflection while allowing light scattered by etched grain boundaries in a near range from specular reflection to pass through, and optical detection devices for detecting and measuring intensities of the respective intermediate scattered light and near specular scattered light. A center blocking aperture or filter can be used to screen out specular reflected light, which would be reflected by nondefect portions of the polycrystalline material surface. An X-Y translation stage for mounting the polycrystalline material and signal processing and computer equipment accommodate rastor mapping, recording, and displaying of respective dislocation and grain boundary defect densities. A special etch procedure is included, which prepares the polycrystalline material surface to produce distinguishable intermediate and near specular light scattering in patterns that have statistical relevance to the dislocation and grain boundary defect densities. 20 figures.
Lattice QCD Calculation of Hadronic Light-by-Light Scattering.
Green, Jeremy; Gryniuk, Oleksii; von Hippel, Georg; Meyer, Harvey B; Pascalutsa, Vladimir
2015-11-27
We perform a lattice QCD calculation of the hadronic light-by-light scattering amplitude in a broad kinematical range. At forward kinematics, the results are compared to a phenomenological analysis based on dispersive sum rules for light-by-light scattering. The size of the pion pole contribution is investigated for momenta of typical hadronic size. The presented numerical methods can be used to compute the hadronic light-by-light contribution to the anomalous magnetic moment of the muon. Our calculations are carried out in two-flavor QCD with the pion mass in the range of 270-450 MeV and contain so far only the diagrams with fully connected quark lines.
Locally-enhanced light scattering by a monocrystalline silicon wafer
NASA Astrophysics Data System (ADS)
Ma, Li; Zhang, Pan; Li, Zhen-Hua; Liu, Chun-Xiang; Li, Xing; Zhan, Zi-Jun; Ren, Xiao-Rong; He, Chang-Wei; Chen, Chao; Cheng, Chuan-Fu
2018-03-01
We study the optical properties of light scattering by a monocrystalline silicon wafer, by using transparent material to replicate its surface structure and illuminating a fabricated sample with a laser source. The experimental results show that the scattering field contains four spots of concentrated intensity with high local energy, and these spots are distributed at the four vertices of a square with lines of intensity linking adjacent spots. After discussing simulations of and theory about the formation of this light scattering, we conclude that the scattering field is formed by the effects of both geometrical optics and physical optics. Moreover, we calculate the central angle of the spots in the light field, and the result indicates that the locally-enhanced intensity spots have a definite scattering angle. These results may possibly provide a method for improving energy efficiency within mono-Si based solar cells.
Fiber optic light-scattering measurement system for evaluation of embryo viability: model experiment
NASA Astrophysics Data System (ADS)
Itoh, Harumi; Arai, Tsunenori; Kikuchi, Makoto
1996-05-01
We evaluated the particle density detectability and particle size detectivity of our fiber-optic light-scattering measurement system. In order to prevent the multiple pregnancy on current in vitro fertilization-embryo transfer, we have aimed to develop a new quantitative and non- invasive method to select a single viable human embryo. We employed the measurement of mitochondria localization in an embryo, which may have the correlation with development ability. We applied the angular distribution measurement of the light-scattering intensity from the embryo to obtain the information originated from the mitochondria. The latex spheres with a diameter of 1.0 micrometers were used to simulate the scattering intensity of the mitochondria. The measurement probes of our system consisted of two fibers for illumination and sensing. They were arranged at a right angle to a microscope optical axis to measure the angular distribution of the light-scattering intensity. We observed that the light-scattering intensity increased monotonically in the range from 106 to 1010 particles per ml. Since the mitochondria density in a human embryo corresponded to 2.5 X 107 per ml in the measurement chamber, we may measure the mitochondria density in the human embryo. The angular dependence of light-scattering intensity changed with the sphere diameters. This result showed the possibility of the selective measurement of the mitochondria density in the embryo in spite of the presence of the other cell organelle. We think that our light-scattering measurement system might be applicable to the evaluation method for the embryo viability.
Microscopic Imaging and Spectroscopy with Scattered Light
Boustany, Nada N.; Boppart, Stephen A.; Backman, Vadim
2012-01-01
Optical contrast based on elastic scattering interactions between light and matter can be used to probe cellular structure and dynamics, and image tissue architecture. The quantitative nature and high sensitivity of light scattering signals to subtle alterations in tissue morphology, as well as the ability to visualize unstained tissue in vivo, has recently generated significant interest in optical scatter based biosensing and imaging. Here we review the fundamental methodologies used to acquire and interpret optical scatter data. We report on recent findings in this field and present current advances in optical scatter techniques and computational methods. Cellular and tissue data enabled by current advances in optical scatter spectroscopy and imaging stand to impact a variety of biomedical applications including clinical tissue diagnosis, in vivo imaging, drug discovery and basic cell biology. PMID:20617940
The role of water in the formation of reversed micelles: An antimicellization agent
Yu, Z.-J.; Zhou, N.-F.; Neuman, R.D.
1992-01-01
Micellization of sodium bis(2-ethylhexyl) phosphate in n-heptane has been studied under controlled environmental conditions by dynamic and static light scattering. The results clearly show that a trace amount of water has a very dramatic effect on reversed micellization. In contrast with results in the literature, water can function as an antimicellization agent. The generality of and the evidence for supporting the current view that water is a prerequisite for the formation of reversed micelles are discussed and criticized. ?? 1992 American Chemical Society.
Atomic Dynamics in Simple Liquid: de Gennes Narrowing Revisited
NASA Astrophysics Data System (ADS)
Wu, Bin; Iwashita, Takuya; Egami, Takeshi
2018-03-01
The de Gennes narrowing phenomenon is frequently observed by neutron or x -ray scattering measurements of the dynamics of complex systems, such as liquids, proteins, colloids, and polymers. The characteristic slowing down of dynamics in the vicinity of the maximum of the total scattering intensity is commonly attributed to enhanced cooperativity. In this Letter, we present an alternative view on its origin through the examination of the time-dependent pair correlation function, the van Hove correlation function, for a model liquid in two, three, and four dimensions. We find that the relaxation time increases monotonically with distance and the dependence on distance varies with dimension. We propose a heuristic explanation of this dependence based on a simple geometrical model. This finding sheds new light on the interpretation of the de Gennes narrowing phenomenon and the α -relaxation time.
2000-01-20
Traveling west to east, the full moon, viewed from Merritt Island, Fla., at 10:18 p.m. EST, begins moving into the Earth's shadow, at the start of a lunar eclipse. Eclipses occur when the Sun, Earth and Moon line up. They are rare because the Moon usually passes above or below the imaginary line connecting Earth and the Sun. The Earth casts a shadow that the Moon can pass through -when it does, it is called a lunar eclipse. They can only occur when the moon is "full." During a total lunar eclipse the Moon takes on a dark red color because it is being lighted slightly by sunlight passing through the Earth's atmosphere and this light has the blue component preferentially scattered out (this is also why the sky appears blue from the surface of the Earth), leaving faint reddish light to illuminate the Moon during the eclipse
2000-01-20
Viewed from Merritt Island, Fla., at 11:25 p.m. EST, the full moon, traveling west to east, is nearly completely in the Earth's shadow, producing a lunar eclipse. Eclipses occur when the Sun, Earth and Moon line up. They are rare because the Moon usually passes above or below the imaginary line connecting Earth and the Sun. The Earth casts a shadow that the Moon can pass through -when it does, it is called a lunar eclipse. They can only occur when the moon is "full." During a total lunar eclipse the Moon takes on a dark red color because it is being lighted slightly by sunlight passing through the Earth's atmosphere and this light has the blue component preferentially scattered out (this is also why the sky appears blue from the surface of the Earth), leaving faint reddish light to illuminate the Moon during the eclipse
2000-01-20
Viewed from Merritt Island, Fla., at 10:59 p.m. EST, the full moon, traveling west to east, is three-quarters of the way into the Earth's shadow during a lunar eclipse. Eclipses occur when the Sun, Earth and Moon line up. They are rare because the Moon usually passes above or below the imaginary line connecting Earth and the Sun. The Earth casts a shadow that the Moon can pass through -when it does, it is called a lunar eclipse. They can only occur when the moon is "full." During a total lunar eclipse the Moon takes on a dark red color because it is being lighted slightly by sunlight passing through the Earth's atmosphere and this light has the blue component preferentially scattered out (this is also why the sky appears blue from the surface of the Earth), leaving faint reddish light to illuminate the Moon during the eclipse
Photopolarimetry of scattering surfaces and their interpretation by computer model
NASA Technical Reports Server (NTRS)
Wolff, M.
1979-01-01
Wolff's computer model of a rough planetary surface was simplified and revised. Close adherence to the actual geometry of a pitted surface and the inclusion of a function for diffuse light resulted in a quantitative model comparable to observations by planetary satellites and asteroids. A function is also derived to describe diffuse light emitted from a particulate surface. The function is in terms of the indices of refraction of the surface material, particle size, and viewing angles. Computer-generated plots describe the observable and theoretical light components for the Moon, Mercury, Mars and a spectrum of asteroids. Other plots describe the effects of changing surface material properties. Mathematical results are generated to relate the parameters of the negative polarization branch to the properties of surface pitting. An explanation is offered for the polarization of the rings of Saturn, and the average diameter of ring objects is found to be 30 to 40 centimeters.
Strain-induced three-photon effects
NASA Astrophysics Data System (ADS)
Jeong, Jae-Woo; Shin, Sung-Chul; Lyubchanskii, I. L.; Varyukhin, V. N.
2000-11-01
Strain-induced three-photon effects such as optical second-harmonic generation and hyper-Rayleigh light scattering, characterized by electromagnetic radiation at the double frequency of an incident light, are phenomenologically investigated by adopting a nonlinear photoelastic interaction. The relations between the strain and the nonlinear optical susceptibility for crystal surfaces with point symmetries of 4mm and 3m are described by a symmetry analysis of the nonlinear photoelastic tensor. We theoretically demonstrate a possibility of determining the strain components by measuring the rotational anisotropy of radiation at the second-harmonic frequency. Hyper-Rayleigh light scattering by dislocation strain is also described using a nonlinear photoelastic tensor. The angular dependencies of light scattered at the double frequency of an incident light for different scattering geometries are analyzed.
NASA Astrophysics Data System (ADS)
Oikarinen, L.
Solar UV and visible radiation scattered at the limb of the Earth's atmosphere is used for measuring density profiles of atmosperic trace gases. For example, the OSIRIS instrument on Odin and SCIAMACHY on Envisat use this technique. A limb-viewing instrument does not see Earth's surface or tropospheric clouds directly. However, in- direct light reflected from the surface or low altitude clouds can make up tens of per cents of the signal. Furthermore, the surface area that contributes to limb intensity ex- tends over 1000 km along the instrument line-of-sight and 200 km across it. Over this area surface reflectivity can vary from almost 0% to 100%. Inaccurate modelling of reflected intensity is a potential source of error in the trace gas retrieval. Generally, radiative transfer models used for analysing limb measure- ments have to assume that the surface has a constant albedo. We have used a three- dimensional Monte Carlo radiative transfer model to study the effects of surface vari- ation to limb radiance. Based on the simulations, we have developed an approximate method for averaging surface albedo for limb scattering measurements with the help of a simple single scattering radiative transfer model.
NASA Technical Reports Server (NTRS)
Eguchi, Satoshi; Ueda, Yoshihiro; Terashima, Yuichi; Mushotzky, Richard F.; Tueller, Jack
2009-01-01
We present a systematic spectral analysis with Suzaku of six AGNs detected in the Swift/BAT hard X-ray (15-200 keV) survey, Swift J0138.6-4001, J0255.2-0011, J0350.1-5019, J0505.7-2348, J0601.9-8636, and J1628.1-5145. This is considered to be a representative sample of new AGNs without X-ray spectral information before the BAT survey. We find that the 0.5-200 keV spectra of these sources can be uniformly fit with a base model consisting of heavily absorbed (log NH >23.5/sq cm) transmitted components, scattered lights, a reflection component, and an iron-K emission line. There are two distinct groups, three "new type" AGNs (including the two sources reported by Ueda et al. 2007) with an extremely small scattered fraction (f(sub scat) < 0:5%) and strong reflection component (R = omega/2pi > or equal to 0.8 where omega is the solid angle of the reflector), and three "classical type" ones with f(sub scat > 0.5% and R < or approx. 0.8. The spectral parameters suggest that the new type has an optically thick torus for Thomson scattering (N(sub H) approx. 10(exp 25)/sq cm) with a small opening angle theta approx. 20deg viewed in a rather face-on geometry, while the classical type has a thin torus (N(sub H) approx. 10(exp 23-24)/sq cm) with theta > or approx. 30deg. We infer that a significant number of new type AGNs with an edge-on view is missing in the current all-sky hard X-ray surveys. Subject headings: galaxies: active . gamma rays: observations . X-rays: galaxies . X-rays: general
Light scattering apparatus and method for determining radiation exposure to plastic detectors
Hermes, Robert E.
2002-01-01
An improved system and method of analyzing cumulative radiation exposure registered as pits on track etch foils of radiation dosimeters. The light scattering apparatus and method of the present invention increases the speed of analysis while it also provides the ability to analyze exposure levels beyond that which may be properly measured with conventional techniques. Dosimeters often contain small plastic sheets that register accumulated damage when exposed to a radiation source. When the plastic sheet from the dosimeter is chemically etched, a track etch foil is produced wherein pits or holes are created in the plastic. The number of these pits, or holes, per unit of area (pit density) correspond to the amount of cumulative radiation exposure which is being optically measured by the apparatus. To measure the cumulative radiation exposure of a track etch foil a high intensity collimated beam is passed through foil such that the pits and holes within the track etch foil cause a portion of the impinging light beam to become scattered upon exit. The scattered light is focused with a lens, while the primary collimated light beam (unscattered light) is blocked. The scattered light is focused by the lens onto an optical detector capable of registering the optical power of the scattered light which corresponds to the cumulative radiation to which the track etch foil has been exposed.
MISR Global Images See the Light of Day
NASA Technical Reports Server (NTRS)
2002-01-01
As of July 31, 2002, global multi-angle, multi-spectral radiance products are available from the MISR instrument aboard the Terra satellite. Measuring the radiative properties of different types of surfaces, clouds and atmospheric particulates is an important step toward understanding the Earth's climate system. These images are among the first planet-wide summary views to be publicly released from the Multi-angle Imaging SpectroRadiometer experiment. Data for these images were collected during the month of March 2002, and each pixel represents monthly-averaged daylight radiances from an area measuring 1/2 degree in latitude by 1/2 degree in longitude.The top panel is from MISR's nadir (vertical-viewing) camera and combines data from the red, green and blue spectral bands to create a natural color image. The central view combines near-infrared, red, and green spectral data to create a false-color rendition that enhances highly vegetated terrain. It takes 9 days for MISR to view the entire globe, and only areas within 8 degrees of latitude of the north and south poles are not observed due to the Terra orbit inclination. Because a single pole-to-pole swath of MISR data is just 400 kilometers wide, multiple swaths must be mosaiced to create these global views. Discontinuities appear in some cloud patterns as a consequence of changes in cloud cover from one day to another.The lower panel is a composite in which red, green, and blue radiances from MISR's 70-degree forward-viewing camera are displayed in the northern hemisphere, and radiances from the 70-degree backward-viewing camera are displayed in the southern hemisphere. At the March equinox (spring in the northern hemisphere, autumn in the southern hemisphere), the Sun is near the equator. Therefore, both oblique angles are observing the Earth in 'forward scattering', particularly at high latitudes. Forward scattering occurs when you (or MISR) observe an object with the Sun at a point in the sky that is in front of you. Relative to the nadir view, this geometry accentuates the appearance of polar clouds, and can even reveal clouds that are invisible in the nadir direction. In relatively clear ocean areas, the oblique-angle composite is generally brighter than its nadir counterpart due to enhanced reflection of light by atmospheric particulates.MISR was built and is managed by NASA's Jet Propulsion Laboratory, Pasadena, CA, for NASA's Office of Earth Science, Washington, DC. The Terra satellite is managed by NASA's Goddard Space Flight Center, Greenbelt, MD. JPL is a division of the California Institute of Technology.Aerosol particle size distribution in the stratosphere retrieved from SCIAMACHY limb measurements
NASA Astrophysics Data System (ADS)
Malinina, Elizaveta; Rozanov, Alexei; Rozanov, Vladimir; Liebing, Patricia; Bovensmann, Heinrich; Burrows, John P.
2018-04-01
Anisotropic Light Scattering from Ferrofluids
NASA Astrophysics Data System (ADS)
Rablau, Corneliu; Vaishnava, Prem; Naik, Ratna; Lawes, Gavin; Tackett, Ron; Sudakar, C.
2008-03-01
We have investigated the light scattering in DC magnetic fields from aqueous suspensions of Fe3O4 nanoparticles coated with tetra methyl ammonium hydroxide and γ-Fe2O3 nanoparticles embedded in alginate hydrogel. For Fe3O4 ferrofluid, anomalous light scattering behavior was observed when light propagated both parallel and perpendicular to the magnetic fields. This behavior is attributed to the alignment and aggregation of the nanoparticles in chain-like structures. A very different light scattering behavior was observed for γ-Fe2O3 alginate sample where, under the similar conditions, the application of the magnetic field produced no structured change in scattering. We attribute this difference to the absence of chain-like structures and constrained mobility of iron nanoparticles in the alginate sample. The observation is in agreement with our relaxation and dissipative heating results^1 where both samples exhibited Neel relaxation but only the Fe3O4 ferrofluid showed Brownian relaxation. The results suggest that Brownian relaxation and nanoparticle mobility are important for producing non-linear light scattering in such systems. ^1P.P. Vaishnava, R. Tackett, A. Dixit, C. Sudakar, R. Naik, and G. Lawes, J. Appl. Phys. 102, 063914 (2007).
NASA Astrophysics Data System (ADS)
Shao, Hongbing
Software testing with scientific software systems often suffers from test oracle problem, i.e., lack of test oracles. Amsterdam discrete dipole approximation code (ADDA) is a scientific software system that can be used to simulate light scattering of scatterers of various types. Testing of ADDA suffers from "test oracle problem". In this thesis work, I established a testing framework to test scientific software systems and evaluated this framework using ADDA as a case study. To test ADDA, I first used CMMIE code as the pseudo oracle to test ADDA in simulating light scattering of a homogeneous sphere scatterer. Comparable results were obtained between ADDA and CMMIE code. This validated ADDA for use with homogeneous sphere scatterers. Then I used experimental result obtained for light scattering of a homogeneous sphere to validate use of ADDA with sphere scatterers. ADDA produced light scattering simulation comparable to the experimentally measured result. This further validated the use of ADDA for simulating light scattering of sphere scatterers. Then I used metamorphic testing to generate test cases covering scatterers of various geometries, orientations, homogeneity or non-homogeneity. ADDA was tested under each of these test cases and all tests passed. The use of statistical analysis together with metamorphic testing is discussed as a future direction. In short, using ADDA as a case study, I established a testing framework, including use of pseudo oracles, experimental results and the metamorphic testing techniques to test scientific software systems that suffer from test oracle problems. Each of these techniques is necessary and contributes to the testing of the software under test.
Veligdan, James Thomas
1997-01-01
An optical display includes a plurality of optical waveguides each including a cladding bound core for guiding internal display light between first and second opposite ends by total internal reflection. The waveguides are stacked together to define a collective display thickness. Each of the cores includes a heterogeneous portion defining a light scattering site disposed longitudinally between the first and second ends. Adjacent ones of the sites are longitudinally offset from each other for forming a longitudinal internal image display over the display thickness upon scattering of internal display light thereagainst for generating a display image. In a preferred embodiment, the waveguides and scattering sites are transparent for transmitting therethrough an external image in superposition with the display image formed by scattering the internal light off the scattering sites for defining a heads up display.
Stray light analysis for the Thomson scattering diagnostic of the ETE Tokamak
DOE Office of Scientific and Technical Information (OSTI.GOV)
Berni, L. A.; Albuquerque, B. F. C.
2010-12-15
Thomson scattering is a well-established diagnostic for measuring local electron temperature and density in fusion plasma, but this technique is particularly difficult to implement due to stray light that can easily mask the scattered signal from plasma. To mitigate this problem in the multipoint Thomson scattering system implemented at the ETE (Experimento Tokamak Esferico) a detailed stray light analysis was performed. The diagnostic system was simulated in ZEMAX software and scattering profiles of the mechanical parts were measured in the laboratory in order to have near realistic results. From simulation, it was possible to identify the main points that contributemore » to the stray signals and changes in the dump were implemented reducing the stray light signals up to 60 times.« less
Scaled-model guidelines for formation-flying solar coronagraph missions.
Landini, Federico; Romoli, Marco; Baccani, Cristian; Focardi, Mauro; Pancrazzi, Maurizio; Galano, Damien; Kirschner, Volker
2016-02-15
Stray light suppression is the main concern in designing a solar coronagraph. The main contribution to the stray light for an externally occulted space-borne solar coronagraph is the light diffracted by the occulter and scattered by the optics. It is mandatory to carefully evaluate the diffraction generated by an external occulter and the impact that it has on the stray light signal on the focal plane. The scientific need for observations to cover a large portion of the heliosphere with an inner field of view as close as possible to the photospheric limb supports the ambition of launching formation-flying giant solar coronagraphs. Their dimension prevents the possibility of replicating the flight geometry in a clean laboratory environment, and the strong need for a scaled model is thus envisaged. The problem of scaling a coronagraph has already been faced for exoplanets, for a single point source on axis at infinity. We face the problem here by adopting an original approach and by introducing the scaling of the solar disk as an extended source.
Scattered, extinguished, emitted: Three views of the dust in Perseus
NASA Astrophysics Data System (ADS)
Foster, Jonathan Bruce
Dust in star-forming regions is both a blessing and a curse. By shrouding young stars it inhibits our study of their birth, yet without dust we would have an impoverished view of the structure of the molecular cloud before it collapses to form a protostar--the initial conditions of the problem of star formation. Though less than 1% of the mass of a molecular cloud, dust is a reliable tracer of the invisible H 2 which makes up the vast majority of the material. Other molecules can trace the H 2 distribution, and are useful in the appropriate regime, but all are confounded by the complications of chemistry, excitation conditions, and depletion, processes which have little effect on dust. Interpreting observations of dust is not entirely straightforward. We do not have a comprehensive theory of dust which explains the size distribution and mineralogical composition of dust in the diverse environments where it is present, from the diffuse ISM to the proto-planetary disks around young stars. Lacking such a theory, it is surprising that models of dust are nonetheless able to reproduce many of the observational constraints imposed upon them. Among these constraints are direct capture of dust grains, spectral features, extinction of background light, scattering, and thermal emission. In this thesis I (1) describe a method to use scattered ambient galactic light to map dense cores with unprecedented high resolution; (2) extend near-infrared extinction mapping by incorporating background galaxies; (3) demonstrate a relation between column density and changes in the extinction law, which is evidence of grain growth; (4) report on a study using NH 3 temperatures to more precisely interpret a thermal emission map at 1.1-mm; and (5) apply all these different techniques to a single starless region in order to compare them and learn something both about dust and the initial conditions of star formation.
Light in flight photography and applications (Conference Presentation)
NASA Astrophysics Data System (ADS)
Faccio, Daniele
2017-02-01
The first successful attempts (Abramson) at capturing light in flight relied on the holographic interference between the ``object'' beam scattered from a screen and a short reference pulse propagating at an angle, acting as an ultrafast shutter cite{egg}. This interference pattern was recorded on a photographic plate or film and allowed the visualisation of light as it propagated through complex environments with unprecedented temporal and spatial resolution. More recently, advances in ultrafast camera technology and in particular the use of picosecond resolution streak cameras allowed the direct digital recording of a light pulse propagating through a plastic bottle (Rasker at el.). This represented a remarkable step forward as it provided the first ever video recording (in the traditional sense with which one intends a video, i.e. something that can be played back directly on a screen and saved in digital format) of a pulse of light in flight. We will discuss a different technology that is based on an imaging camera with a pixel array in which each individual pixel is a single photon avalanche diode (SPAD). SPADs offer both sensitivity to single photons and picosecond temporal resolution of the photon arrival time (with respect to a trigger event). When adding imaging capability, SPAD arrays can deliver videos of light pulse propagating in free space, without the need for a scattering medium or diffuser as in all previous work (Gariepy et al). This capability can then be harnessed for a variety of applications. We will discuss the details of SPAD camera detection of moving objects (e.g. human beings) that are hidden from view and then conclude with a discussion of future perspectives in the field of bio-imaging.
The Effect of Incident Light Polarization on Vegetation Bidirectional Reflectance Factor
NASA Technical Reports Server (NTRS)
Georgiev, Georgi T.; Thome, Kurt; Ranson, Kurtis J.; King, Michael D.; Butler, James J.
2010-01-01
The Laboratory-based Bidirectional Reflectance Factor (BRF) polarization study of vegetation is presented in this paper. The BRF was measured using a short-arc Xenon lamp/monochromator assembly producing an incoherent, tunable light source with a well-defined spectral bandpass at visible and near-infrared wavelengths of interest at 470 nm and 870 nm and coherent light source at 1.656 microns. All vegetation samples were measured using P and S linearly polarized incident light over a range of incident and scatter angles. By comparing these results, we quantitatively examine how the BRF of the samples depends on the polarization of the incident light. The differences are significant, depend strongly on the incident and scatter angles, and can be as high as 120% at 67 deg incident and 470nm. The global nature of Earth's processes requires consistent long-term calibration of all instruments involved in data retrieval. The BRF defines the reflection characteristics of Earth surface. It provides the reflectance of a target in a specific direction as a function of illumination and viewing geometry. The BRF is a function of wavelength and reflects the structural and optical properties of the surface. Various space and airborne radiometric and imaging remote sensing instruments are used in the remote sensing characterization of vegetation canopies and soils, oceans, or especially large pollution sources. The satellite data is validated through comparison with airborne, ground-based and laboratory-based data in an effort to fully understand the vegetation canopy reflectance, The Sun's light is assumed to be unpolarized at the top of the atmosphere; however it becomes polarized to some degree due to atmospheric effects by the time it reaches the vegetation canopy. Although there are numerous atmospheric correction models, laboratory data is needed for model verification and improvement.
Mindukshev, Igor; Gambaryan, Stepan; Kehrer, Linda; Schuetz, Claudia; Kobsar, Anna; Rukoyatkina, Natalia; Nikolaev, Viacheslav O; Krivchenko, Alexander; Watson, Steve P; Walter, Ulrich; Geiger, Joerg
2012-07-01
Determinations of platelet receptor functions are indispensable diagnostic indicators of cardiovascular and hemostatic diseases including hereditary and acquired receptor defects and receptor responses to drugs. However, presently available techniques for assessing platelet function have some disadvantages, such as low sensitivity and the requirement of large sample sizes and unphysiologically high agonist concentrations. Our goal was to develop and initially characterize a new technique designed to quantitatively analyze platelet receptor activation and platelet function on the basis of measuring changes in low angle light scattering. We developed a novel technique based on low angle light scattering registering changes in light scattering at a range of different angles in platelet suspensions during activation. The method proved to be highly sensitive for simultaneous real time detection of changes in size and shape of platelets during activation. Unlike commonly-used methods, the light scattering method could detect platelet shape change and aggregation in response to nanomolar concentrations of extracellular nucleotides. Furthermore, our results demonstrate that the advantages of the light scattering method make it a choice method for platelet receptor monitoring and for investigation of both murine and human platelets in disease models. Our data demonstrate the suitability and superiority of this new low angle light scattering method for comprehensive analyses of platelet receptors and functions. This highly sensitive, quantitative, and online detection of essential physiological, pathophysiological and pharmacological-response properties of human and mouse platelets is a significant improvement over conventional techniques.
Simulating propagation of coherent light in random media using the Fredholm type integral equation
NASA Astrophysics Data System (ADS)
Kraszewski, Maciej; Pluciński, Jerzy
2017-06-01
Studying propagation of light in random scattering materials is important for both basic and applied research. Such studies often require usage of numerical method for simulating behavior of light beams in random media. However, if such simulations require consideration of coherence properties of light, they may become a complex numerical problems. There are well established methods for simulating multiple scattering of light (e.g. Radiative Transfer Theory and Monte Carlo methods) but they do not treat coherence properties of light directly. Some variations of these methods allows to predict behavior of coherent light but only for an averaged realization of the scattering medium. This limits their application in studying many physical phenomena connected to a specific distribution of scattering particles (e.g. laser speckle). In general, numerical simulation of coherent light propagation in a specific realization of random medium is a time- and memory-consuming problem. The goal of the presented research was to develop new efficient method for solving this problem. The method, presented in our earlier works, is based on solving the Fredholm type integral equation, which describes multiple light scattering process. This equation can be discretized and solved numerically using various algorithms e.g. by direct solving the corresponding linear equations system, as well as by using iterative or Monte Carlo solvers. Here we present recent development of this method including its comparison with well-known analytical results and a finite-difference type simulations. We also present extension of the method for problems of multiple scattering of a polarized light on large spherical particles that joins presented mathematical formalism with Mie theory.
Particle measurement systems and methods
Steele, Paul T [Livermore, CA
2011-10-04
A system according to one embodiment includes a light source for generating light fringes; a sampling mechanism for directing a particle through the light fringes; and at least one light detector for detecting light scattered by the particle as the particle passes through the light fringes. A method according to one embodiment includes generating light fringes using a light source; directing a particle through the light fringes; and detecting light scattered by the particle as the particle passes through the light fringes using at least one light detector.
Observation of two-beam collective scattering phenomena in a Bose-Einstein condensate
NASA Astrophysics Data System (ADS)
Dimitrova, Ivana; Lunden, William; Amato-Grill, Jesse; Jepsen, Niklas; Yu, Yichao; Messer, Michael; Rigaldo, Thomas; Puentes, Graciana; Weld, David; Ketterle, Wolfgang
2017-11-01
Different regimes of collective light scattering are observed when an elongated Bose-Einstein condensate is pumped by two noninterfering beams counterpropagating along its long axis. In the limit of small Rayleigh scattering rates, the presence of a second pump beam suppresses superradiance, whereas at large Rayleigh scattering rates it lowers the effective threshold power for collective light scattering. In the latter regime, the quench dynamics of the two-beam system are oscillatory, compared to monotonic in the single-beam case. In addition, the dependence on power, detuning, and atom number is explored. The observed features of the two-beam system qualitatively agree with the recent theoretical prediction of a supersolid crystalline phase of light and matter at large Rayleigh scattering rates.
Ho, Derek; Kim, Sanghoon; Drake, Tyler K.; Eldridge, Will J.; Wax, Adam
2014-01-01
We present a fast approach for size determination of spherical scatterers using the continuous wavelet transform of the angular light scattering profile to address the computational limitations of previously developed sizing techniques. The potential accuracy, speed, and robustness of the algorithm were determined in simulated models of scattering by polystyrene beads and cells. The algorithm was tested experimentally on angular light scattering data from polystyrene bead phantoms and MCF-7 breast cancer cells using a 2D a/LCI system. Theoretical sizing of simulated profiles of beads and cells produced strong fits between calculated and actual size (r2 = 0.9969 and r2 = 0.9979 respectively), and experimental size determinations were accurate to within one micron. PMID:25360350
Detection of internal structure by scattered light intensity: Application to kidney cell sorting
NASA Technical Reports Server (NTRS)
Goolsby, C. L.; Kunze, M. E.
1985-01-01
Scattered light measurements in flow cytometry were sucessfully used to distinguish cells on the basis of differing morphology and internal structure. Differences in scattered light patterns due to changes in internal structure would be expected to occur at large scattering angles. Practically, the results of these calculations suggest that in experimental situations an array of detectors would be useful. Although in general the detection of the scattered light intensity at several intervals within the 10 to 60 region would be sufficient, there are many examples where increased sensitivity could be acheived at other angles. The ability to measure at many different angular intervals would allow the experimenter to empirically select the optimum intervals for the varying conditions of cell size, N/C ratio, granule size and internal structure from sample to sample. The feasibility of making scattered light measurements at many different intervals in flow cytometry was demonstrated. The implementation of simplified versions of these techniques in conjunction with independant measurements of cell size could potentially improve the usefulness of flow cytometry in the study of the internal structure of cells.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Schoenfeld, A; Poppinga, D; Poppe, B
Purpose: This study aims to investigate the optical properties of radiochromic EBT3 films on exposure to polarized incident light. Methods: An optical table setup was used to investigate the properties of exposed and unexposed EBT3 films. The films were placed with their long side horizontally and illuminated with polarized incident white light. The polarization of light with the electrical vector pointing vertically is referred to as 0°, accordingly horizontal orientation corresponds to 90°. The light transmission was measured depending on the polarization angle of the incident light and the polarization of a polarizer in front of the detector. Secondly, themore » scattering properties of exposed and unexposed films were measured by placing a plane convex lens behind the films and a screen in its focal plane. Thereby, the distribution of the scattering angles appears as an intensity map on the screen. The distributions of scattering angles caused by EBT3 films and by neutral density filters were compared. Results: EBT3 films show a strong dependence of the light transmission on the polarization of the incident light. With both polarizers parallel, a peak transmission was found at 90° orientation of the polarizers. With the rear polarizer at right angles with the front polarizer, peak transmissions were found at front polarizer orientations 45° and 135°. The scattering appears to be anisotropic with a preference direction parallel to the long side of the film. The portion of scattered light and the half value scattering angle both increase with the dose on the film. Conclusion: EBT3 films show dose dependent changes in polarized light transmission and anisotropic light scattering. These effects impair the light absorption measurements on exposed films performed with commercial flatbed scanners and are causing the well-known artifacts of radiochromic film dosimetry with flatbed scanners, the “orientation effect” and the “parabola effect”.« less
Development of a multispectral light-scatter sensor for bacterial colonies
USDA-ARS?s Scientific Manuscript database
We report a multispectral elastic-light-scatter instrument that can simultaneously detect three-wavelength scatter patterns and associated optical densities from individual bacterial colonies, overcoming the limits of the single-wavelength predecessor. Absorption measurements on liquid bacterial sam...
Apparatus and method for spectroscopic analysis of scattering media
Strobl, Karlheinz; Bigio, Irving J.; Loree, Thomas R.
1994-01-01
Apparatus and method for spectroscopic analysis of scattering media. Subtle differences in materials have been found to be detectable from plots of intensity as a function of wavelength of collected emitted and scattered light versus wavelength of excitation light.
Features in the speckle correlations of light scattered from volume-disordered dielectric media
NASA Astrophysics Data System (ADS)
Malyshkin, V.; McGurn, A. R.; Maradudin, A. A.
1999-03-01
A diagrammatic perturbation theory approach, based on a scalar wave treatment, is used to study the scattering of light of frequency ω from a volume disordered dielectric medium. The dielectric medium is described by a position-dependent dielectric constant of the form ɛ(r-->)=ɛ(ω)+δɛ(r-->), where ɛ(ω) does not depend on r-->, and δɛ(r-->) is a zero-mean Gaussian random process defined by <δɛ(r-->)δɛ(r-->')>=σ2 exp(-\\|r-->-r-->'\\|2/a2), where the angle brackets denote an average over the ensemble of realizations of δɛ(r-->), a is the correlation length of the disorder, and σ is the root mean square deviation of the dielectric constant from its average value ɛ(ω). The speckle correlation function C(q-->,k-->\\|q-->',k-->')=<[I(q-->\\|k-->)-\\|k-->)>][I(q-->'\\|k-->')-'\\|k-->')]> where I(q-->\\|k-->) is proportional to the differential-scattering coefficient for the scattering of light of incident wave vector k--> into light of wave vector q--> is computed. In these calculations the contributions associated with both ladder and maximally crossed diagrams are summed in a Feynman diagram treatment of the speckle correlator, in the approximation that only s-wave-scattering terms are retained. Results are presented for the differential-scattering coefficient of light scattered from the disordered medium, which displays the phenomenon of enhanced backscattering, and for the correlator C in the approximation where C=C(1)+C(10)+C(1.5). The contribution C(1) is proportional to δ(q-->-k-->-q-->'+k-->') and describes the memory and time-reversed memory effects. C(10) is proportional to δ(q-->-k-->+q-->'-k-->'), while C(1.5) is unrestricted in its dependence on q-->,k-->,q-->',k-->'. The latter two contributions have recently been treated in the scattering of light from randomly rough surfaces, but have not been previously treated in the scattering of light by volume disordered media. A number of peaks associated with resonant processes are observed in C(1.5) considered as a function of the wave vectors of the incident and scattered light.
NASA Astrophysics Data System (ADS)
Nishiyama, T.; Kataoka, J.; Kishimoto, A.; Fujita, T.; Iwamoto, Y.; Taya, T.; Ohsuka, S.; Nakamura, S.; Hirayanagi, M.; Sakurai, N.; Adachi, S.; Uchiyama, T.
2014-12-01
After the Japanese nuclear disaster in 2011, large amounts of radioactive isotopes were released and still remain a serious problem in Japan. Consequently, various gamma cameras are being developed to help identify radiation hotspots and ensure effective decontamination operation. The Compton camera utilizes the kinematics of Compton scattering to contract images without using a mechanical collimator, and features a wide field of view. For instance, we have developed a novel Compton camera that features a small size (13 × 14 × 15 cm3) and light weight (1.9 kg), but which also achieves high sensitivity thanks to Ce:GAGG scintillators optically coupled wiith MPPC arrays. By definition, in such a Compton camera, gamma rays are expected to scatter in the ``scatterer'' and then be fully absorbed in the ``absorber'' (in what is called a forward-scattered event). However, high energy gamma rays often interact with the detector in the opposite direction - initially scattered in the absorber and then absorbed in the scatterer - in what is called a ``back-scattered'' event. Any contamination of such back-scattered events is known to substantially degrade the quality of gamma-ray images, but determining the order of gamma-ray interaction based solely on energy deposits in the scatterer and absorber is quite difficult. For this reason, we propose a novel yet simple Compton camera design that includes a rear-panel shield (a few mm thick) consisting of W or Pb located just behind the scatterer. Since the energy of scattered gamma rays in back-scattered events is much lower than that in forward-scattered events, we can effectively discriminate and reduce back-scattered events to improve the signal-to-noise ratio in the images. This paper presents our detailed optimization of the rear-panel shield using Geant4 simulation, and describes a demonstration test using our Compton camera.
Wilcox, R.B.
1991-09-10
A planar transparent light conducting means and an improved optically activated electrical switch made using the novel light conducting means are disclosed. The light conducting means further comprise light scattering means on one or more opposite planar surfaces thereof to transmit light from the light conducting means into adjacent media and reflective means on other surfaces of the light conducting means not containing the light scattering means. The optically activated electrical switch comprises at least two stacked photoconductive wafers, each having electrodes formed on both surfaces thereof, and separated by the planar transparent light conducting means. The light scattering means on the light conducting means face surfaces of the wafers not covered by the electrodes to transmit light from the light conducting means into the photoconductive wafers to uniformly illuminate and activate the switch. 11 figures.
Wilcox, Russell B.
1991-01-01
A planar transparent light conducting means and an improved optically activated electrical switch made using the novel light conducting means are disclosed. The light conducting means further comprise light scattering means on one or more opposite planar surfaces thereof to transmit light from the light conducting means into adjacent media and reflective means on other surfaces of the light conducting means not containing the light scattering means. The optically activated electrical switch comprises at least two stacked photoconductive wafers, each having electrodes formed on both surfaces thereof, and separated by the planar transparent light conducting means. The light scattering means on the light conducting means face surfaces of the wafers not covered by the electrodes to transmit light from the light conducting means into the photoconductive wafers to uniformly illuminate and activate the switch.
Kristin Lewis; William P. Arnott; Hans Moosmuller; Cyle E. Wold
2008-01-01
A dual-wavelength photoacoustic instrument operating at 405 and 870 nm was used during the 2006 Fire Lab at Missoula Experiment to measure light scattering and absorption by smoke from the combustion of a variety of biomass fuels. Simultaneous measurements of aerosol light scattering by reciprocal nephelometry within the instrument's acoustic resonator accompany...
Light Scattering by Marine Particles: Modeling with Non-Spherical Shapes
2011-04-15
scattering by nonspherical particles: application to coccoliths detached from Emiliania huxleyi. Limnology and Oceanography, 46. 1438— 1454,2001. H.R...application to coccoliths detached from Emiliania huxleyi," Limnol. Oceanogr. 46, 1438-1454 (2001). 5. H.R.Gordon, "Backscattering of light from...by coccoliths detached from Emiliania huxleyi," Applied Optics, 48, 6059-6073 (2009). Light scattering by coccoliths detached from Emiliania
Metal Sorbing Vesicles: Light Scattering Characterization and Metal Sorbtion Behavior.
NASA Astrophysics Data System (ADS)
van Zanten, John Hollis
1992-01-01
The research described herein consisted of two parts: light scattering characterization of vesicles and kinetic investigations of metal sorbing vesicles. Static light scattering techniques can be used to determine the geometric size, shape and apparent molecular weight of phosphatidylcholine vesicles in aqueous suspension. A Rayleigh-Gans-Debye (RGD) approximation analysis of multiangle scattered light intensity data yields the size and degree of polydispersity of the vesicles in solution, while the Zimm plot technique provides the radius of gyration and apparent weight-average molecular weight. Together the RGD approximation and Zimm plots can be used to confirm the geometric shape of vesicles and can give a good estimate of the vesicle wall thickness in some cases. Vesicles varying from 40 to 115 nm in diameter have been characterized effectively. The static light scattering measurements indicate that, as expected, phosphatidylcholine vesicles in this size range scatter light as isotropic hollow spheres. Additionally, static and dynamic light scattering measurements have been made and compared with one another. The values for geometric radii determined by static light scattering typically agree with those estimated by dynamic light scattering to within a few percent. Interestingly however, dynamic measurements suggest that there is a significant degree of polydispersity present in the vesicle dispersions, while static measurements indicate near size monodisperse dispersions. Metal sorbing vesicles which harbor ionophores, such as antibiotic A23187 and synthetic carriers, in their bilayer membranes have been produced. These vesicles also encapsulate the chelating compound, nitrilotriacetate, to provide the driving force for metal ion uptake. Very dilute dispersions (on the order of 0.03% w/v) of these metal sorbing vesicles were capable of removing Cd ^{2+} and Pb^{2+ } from dilute aqueous solution (5 ppm and less) and concentrating these metal ions several hundred to more than a thousand fold in the vesicle interior in a few minutes time. Synthetic ionophores were found to preferentially transport Pb^{2+} over Cd^{2+}, thus suggesting that engineered vesicle dispersions can be used as selective separations media. The effect of ionophore concentration, solution pH, solution ionic strength, initial metal ion concentration and vesicle concentration have been investigated.
Polarized Light Scattering from Perfect and Perturbed Surfaces and Fundamental Scattering Systems
1992-02-29
ob- one frequency, an extension of it to multiple-field interac- served in the elastically scattered light emitted from glass tions would follow the...that 8. V CeIll . A. A. Maradudin, A. M. Marvin, and A. R. McGurn, can explain only gross scattering features. It is inde "Some aspects of light...and a surface of index n a 10.0 - 0.01. Such a surface could be made with a series of 1/4-wave dielectric layers on a glass substrate. It Is more
Ye, Yiyang; Chen, Tupei; Zhen, Juyuan; Xu, Chen; Zhang, Jun; Li, Huakai
2018-02-01
The ability to selectively scatter green light is essential for an RGB transparent projection display, and this can be achieved by a silver-core, titania-shell nanostructure (Ag@TiO 2 ), based on the metallic nanoparticle's localized surface plasmon resonance. The ability to selectively scatter green light is shown in a theoretical design, in which structural optimization is included, and is then experimentally verified by characterization of a transparent film produced by dispersing such nanoparticles in a polymer matrix. A visual assessesment indicates that a high-quality green image can be clearly displayed on the transparent film. For completeness, a theoretical design for selective scattering of red light based on Ag@TiO 2 is also shown.
Ichikawa, Hiroko; Kanazawa, So; Yamaguchi, Masami K; Kakigi, Ryusuke
2010-09-27
Adult observers can quickly identify specific actions performed by an invisible actor from the points of lights attached to the actor's head and major joints. Infants are also sensitive to biological motion and prefer to see it depicted by a dynamic point-light display. In detecting biological motion such as whole body and facial movements, neuroimaging studies have demonstrated the involvement of the occipitotemporal cortex, including the superior temporal sulcus (STS). In the present study, we used the point-light display technique and near-infrared spectroscopy (NIRS) to examine infant brain activity while viewing facial biological motion depicted in a point-light display. Dynamic facial point-light displays (PLD) were made from video recordings of three actors making a facial expression of surprise in a dark room. As in Bassili's study, about 80 luminous markers were scattered over the surface of the actor's faces. In the experiment, we measured infant's hemodynamic responses to these displays using NIRS. We hypothesized that infants would show different neural activity for upright and inverted PLD. The responses were compared to the baseline activation during the presentation of individual still images, which were frames extracted from the dynamic PLD. We found that the concentration of oxy-Hb increased in the right temporal area during the presentation of the upright PLD compared to that of the baseline period. This is the first study to demonstrate that infant's brain activity in face processing is induced only by the motion cue of facial movement depicted by dynamic PLD. (c) 2010 Elsevier Ireland Ltd. All rights reserved.
NASA Astrophysics Data System (ADS)
Ortiz-Montalvo, D. L.; Conny, J. M.
2017-12-01
We study the scattering properties of irregularly shaped ambient dust particles. The way in which they scatter and absorb light has implications for aerosol optical remote sensing and aerosol radiative forcing applications. However, understanding light scattering and absorption by non-spherical particles can be very challenging. We used focused ion-beam scanning electron microscopy and energy-dispersive x-ray spectroscopy (FIB-SEM-EDS) to reconstruct three-dimensional (3-D) configurations of dust particles collected from urban and Asian sources. The 3-D reconstructions were then used in a discrete dipole approximation method (DDA) to determine their scattering properties for a range of shapes, sizes, and refractive indices. Scattering properties where obtained using actual-shapes of the particles, as well as, (theoretical) equivalently-sized geometrical shapes like spheres, ellipsoids, cubes, rectangular prisms, and tetrahedrons. We use Q-space analysis to interpret the angular distribution of the scattered light obtained for each particle. Q-space analysis has been recently used to distinguish scattering by particles of different shapes, and it involves plotting the scattered intensity versus the scattering wave vector (q or qR) on a log-log scale, where q = 2ksin(θ/2), k = 2π/λ, and R = particle effective radius. Results from a limited number of particles show that when Q-space analysis is applied, common patterns appear that agree with previous Q-space studies done on ice crystals and other irregularly shaped particles. More specifically, we found similar Q-space regimes including a forward scattering regime of constant intensity when qR < 1, followed by the Guinier regime when qR ≈ 1, which is then followed by a complex power law regime with a -3 slope regime, a transition regime, and then a -4 slope regime. Currently, Q-space comparisons between actual- and geometric shapes are underway with the objective of determining which geometric shape best represents the angular distribution and magnitude of the scattered light. Current work also focuses on the effects of the imaginary part of the refractive index on the light scattering of our dust particles.
An, Seongpil; Jo, Hong Seok; Kim, Yong Il; Song, Kyo Yong; Kim, Min-Woo; Lee, Kyu Bum; Yarin, Alexander L; Yoon, Sam S
2017-07-06
Bioluminescent jellyfish has a unique structure derived from fiber/polymer interfaces that is advantageous for effective light scattering in the dark, deep sea water. Herein, we demonstrate the fabrication of bio-inspired hybrid films by mimicry of the jellyfish's structure, leading to excellent light-scattering performance and defrosting capability. A haze value reaching 59.3% and a heating temperature of up to 292 °C were achieved with the films. Accordingly, the developed surface constitutes an attractive optical device for lighting applications, especially for street or vehicle luminaries for freezing Arctic-climate countries. The morphological details of the hybrid films were revealed by scanning electron microscopy. The light-scattering properties of these films were examined by ultraviolet-visible-infrared spectrophotometry and anti-glare effect analyses. The defrosting performance of the hybrid films was evaluated via heating tests and infra-red observations.
Correlation transfer and diffusion of ultrasound-modulated multiply scattered light.
Sakadzić, Sava; Wang, Lihong V
2006-04-28
We develop a temporal correlation transfer equation (CTE) and a temporal correlation diffusion equation (CDE) for ultrasound-modulated multiply scattered light. These equations can be applied to an optically scattering medium with embedded optically scattering and absorbing objects to calculate the power spectrum of light modulated by a nonuniform ultrasound field. We present an analytical solution based on the CDE and Monte Carlo simulation results for light modulated by a cylinder of ultrasound in an optically scattering slab. We further validate with experimental measurements the numerical calculations for an actual ultrasound field. The CTE and CDE are valid for moderate ultrasound pressures and on a length scale comparable with the optical transport mean-free path. These equations should be applicable to a wide spectrum of conditions for ultrasound-modulated optical tomography of soft biological tissues.
NASA Astrophysics Data System (ADS)
Shvartsman, Leonid D.; Fine, Ilya
2001-06-01
We develop theoretical models of light transmission through whole blood considering RBC aggregation. RBC aggregates are considered to be the main centers of scattering in red/near- infrared spectral region. In pulsatile blood flow the periodic changes of aggregate geometry cause oscillations of light scattering. Thus scattering-assisted mechanism has to be taken into account in pulse oximeter calibration. In case of over-systolic vessel occlusion the size of aggregates grows, and the light transmission rises. Light diffraction on a single scatterer makes the transmission growth non- monotonic for certain spectral range. For the most typical set of aggregate parameters this range corresponds to wavelengths below 760 nm, and this prediction fits well both in vivo and in vitro experimental results. This spectral range depends on the refraction index mismatch and the geometry of aggregates. Both of them may be affected by the chemistry of blood. For instance, changes of glucose and hemoglobin have different effect on light transmission time response. Consequently, their content may be determined from time evolution of optical transmission.
Quantitative photoplethysmography: Lambert-Beer law or inverse function incorporating light scatter.
Cejnar, M; Kobler, H; Hunyor, S N
1993-03-01
Finger blood volume is commonly determined from measurement of infra-red (IR) light transmittance using the Lambert-Beer law of light absorption derived for use in non-scattering media, even when such transmission involves light scatter around the phalangeal bone. Simultaneous IR transmittance and finger volume were measured over the full dynamic range of vascular volumes in seven subjects and outcomes compared with data fitted according to the Lambert-Beer exponential function and an inverse function derived for light attenuation by scattering materials. Curves were fitted by the least-squares method and goodness of fit was compared using standard errors of estimate (SEE). The inverse function gave a better data fit in six of the subjects: mean SEE 1.9 (SD 0.7, range 0.7-2.8) and 4.6 (2.2, 2.0-8.0) respectively (p < 0.02, paired t-test). Thus, when relating IR transmittance to blood volume, as occurs in the finger during measurements of arterial compliance, an inverse function derived from a model of light attenuation by scattering media gives more accurate results than the traditional exponential fit.
Improved fiberoptic spectrophotometer
Tans, P.P.; Lashof, D.A.
1985-04-02
The present invention allows for accurate spectrophotmetric comparison of the Raman scattering from a sample gas with the Raman scattering from a known gas via a novel fiber optic network. The need for complicated electronic of optical circuit balancing, control, or error compensation circuitry is eliminated. The laser cavity is split into two regions, one of which houses the plasma discharge and produces laser power, and the other of which is adapted to house tubes containing the gas samples. Light from the laser source is beamed simultaneously through samples of the reference gas and the unknown gas, and Raman-scattered light is emitted. The Raman-scattered light from the known and unknown mixtures is then alternately passed through a fiber optic network where the various wavelengths are spatially mixed. The mixed light is then passed into a system of light detectors, each of which are adapted to measure one of the wavelengths of light representing a constituent element of the gases. When the test is complete, each gas sample can be assigned a Raman-scattered profile from the data consisting of the ratios each of the constituent elements bear to each other. (LEW)
Ramírez-Duverger, Aldo S; Gaspar-Armenta, Jorge A; García-Llamas, Raúl
2003-08-01
We report experimental results of the resonant scattering of light from a prism-glass/Ag/MgF2/air system with use of the attenuated total reflection technique for p and s polarized light. Two MgF2 film thicknesses were used. The system with the thinner dielectric layer supports two transverse magnetic (TM) and two transverse electric (TE) guided modes at a wavelength of 632.8 nm, and the system with the thicker dielectric layer supports three TM and three TE guided modes. In both cases we found dips in the specular reflection as a function of incident angle that is due to excitation of guided modes in the MgF2 film. The scattered light shows peaks at angles corresponding to the measured excitation of the guided modes. These peaks are due to single-order scattering and occur for any angle of the incident light. All features in the scattering response are enhanced in resonance conditions, and the efficiency of injecting light into the guide is reduced.
Focusing light inside dynamic scattering media with millisecond digital optical phase conjugation
Liu, Yan; Ma, Cheng; Shen, Yuecheng; Shi, Junhui; Wang, Lihong V.
2017-01-01
Wavefront shaping based on digital optical phase conjugation (DOPC) focuses light through or inside scattering media, but the low speed of DOPC prevents it from being applied to thick, living biological tissue. Although a fast DOPC approach was recently developed, the reported single-shot wavefront measurement method does not work when the goal is to focus light inside, instead of through, highly scattering media. Here, using a ferroelectric liquid crystal based spatial light modulator, we develop a simpler but faster DOPC system that focuses light not only through, but also inside scattering media. By controlling 2.6 × 105 optical degrees of freedom, our system focused light through 3 mm thick moving chicken tissue, with a system latency of 3.0 ms. Using ultrasound-guided DOPC, along with a binary wavefront measurement method, our system focused light inside a scattering medium comprising moving tissue with a latency of 6.0 ms, which is one to two orders of magnitude shorter than those of previous digital wavefront shaping systems. Since the demonstrated speed approaches tissue decorrelation rates, this work is an important step toward in vivo deep-tissue non-invasive optical imaging, manipulation, and therapy. PMID:28815194
A real-time photo-realistic rendering algorithm of ocean color based on bio-optical model
NASA Astrophysics Data System (ADS)
Ma, Chunyong; Xu, Shu; Wang, Hongsong; Tian, Fenglin; Chen, Ge
2016-12-01
A real-time photo-realistic rendering algorithm of ocean color is introduced in the paper, which considers the impact of ocean bio-optical model. The ocean bio-optical model mainly involves the phytoplankton, colored dissolved organic material (CDOM), inorganic suspended particle, etc., which have different contributions to absorption and scattering of light. We decompose the emergent light of the ocean surface into the reflected light from the sun and the sky, and the subsurface scattering light. We establish an ocean surface transmission model based on ocean bidirectional reflectance distribution function (BRDF) and the Fresnel law, and this model's outputs would be the incident light parameters of subsurface scattering. Using ocean subsurface scattering algorithm combined with bio-optical model, we compute the scattering light emergent radiation in different directions. Then, we blend the reflection of sunlight and sky light to implement the real-time ocean color rendering in graphics processing unit (GPU). Finally, we use two kinds of radiance reflectance calculated by Hydrolight radiative transfer model and our algorithm to validate the physical reality of our method, and the results show that our algorithm can achieve real-time highly realistic ocean color scenes.
2012-11-28
boiling of the liquid or vaporization of the particle). Light scatters out of the propagation path. • Enhanced absorption from nanoplasmas . 8 I...and thus, nanoplasmas that absorb and scatter the light • NLO behavior is fluence dependent • Uncalibrated measurements of transmitted, absorbed...after the first 1-2 ns • Proposed mechanism: Initial scattering by nanoplasmas followed by additional scattering from bubble growth in the
Xu, Min
2017-01-01
Biological tissue has a complex structure and exhibits rich spectroscopic behavior. There has been no tissue model until now that has been able to account for the observed spectroscopy of tissue light scattering and its anisotropy. Here we present, for the first time, a plum pudding random medium (PPRM) model for biological tissue which succinctly describes tissue as a superposition of distinctive scattering structures (plum) embedded inside a fractal continuous medium of background refractive index fluctuation (pudding). PPRM faithfully reproduces the wavelength dependence of tissue light scattering and attributes the “anomalous” trend in the anisotropy to the plum and the powerlaw dependence of the reduced scattering coefficient to the fractal scattering pudding. Most importantly, PPRM opens up a novel venue of quantifying the tissue architecture and microscopic structures on average from macroscopic probing of the bulk with scattered light alone without tissue excision. We demonstrate this potential by visualizing the fine microscopic structural alterations in breast tissue (adipose, glandular, fibrocystic, fibroadenoma, and ductal carcinoma) deduced from noncontact spectroscopic measurement. PMID:28663913
Dual-domain point diffraction interferometer
Naulleau, Patrick P.; Goldberg, Kenneth Alan
2000-01-01
A hybrid spatial/temporal-domain point diffraction interferometer (referred to as the dual-domain PS/PDI) that is capable of suppressing the scattered-reference-light noise that hinders the conventional PS/PDI is provided. The dual-domain PS/PDI combines the separate noise-suppression capabilities of the widely-used phase-shifting and Fourier-transform fringe pattern analysis methods. The dual-domain PS/PDI relies on both a more restrictive implementation of the image plane PS/PDI mask and a new analysis method to be applied to the interferograms generated and recorded by the modified PS/PDI. The more restrictive PS/PDI mask guarantees the elimination of spatial-frequency crosstalk between the signal and the scattered-light noise arising from scattered-reference-light interfering with the test beam. The new dual-domain analysis method is then used to eliminate scattered-light noise arising from both the scattered-reference-light interfering with the test beam and the scattered-reference-light interfering with the "true" pinhole-diffracted reference light. The dual-domain analysis method has also been demonstrated to provide performance enhancement when using the non-optimized standard PS/PDI design. The dual-domain PS/PDI is essentially a three-tiered filtering system composed of lowpass spatial-filtering the test-beam electric field using the more restrictive PS/PDI mask, bandpass spatial-filtering the individual interferogram irradiance frames making up the phase-shifting series, and bandpass temporal-filtering the phase-shifting series as a whole.
NASA Technical Reports Server (NTRS)
Magi, Brian I.; Hobbs, Peter V.; Schmid, Beat; Redermann, Jens
2003-01-01
Airborne in situ measurements of vertical profiles of aerosol light scattering, light absorption, and single scattering albedo (omega (sub 0)) are presented for a number of locations in southern Africa during the dry, biomass burning season. Features of the profiles include haze layers, clean air slots, and marked decreases in light scattering in passing from the boundary layer into the free troposphere. Frequency distributions of omega (sub 0) reflect the strong influence of smoke from biomass burning. For example, during a period when heavy smoke was advected into the region from the north, the mean value of omega (sub 0) in the boundary layer was 0.81 +/- 0.02 compared to 0.89 +/- 0.03 prior to this intrusion. Comparisons of layer aerosol optical depths derived from the in situ measurements with those measured by a Sun photometer aboard the aircraft show excellent agreement.
Shen, Jian; Deng, Degang; Kong, Weijin; Liu, Shijie; Shen, Zicai; Wei, Chaoyang; He, Hongbo; Shao, Jianda; Fan, Zhengxiu
2006-11-01
By introducing the scattering probability of a subsurface defect (SSD) and statistical distribution functions of SSD radius, refractive index, and position, we derive an extended bidirectional reflectance distribution function (BRDF) from the Jones scattering matrix. This function is applicable to the calculation for comparison with measurement of polarized light-scattering resulting from a SSD. A numerical calculation of the extended BRDF for the case of p-polarized incident light was performed by means of the Monte Carlo method. Our numerical results indicate that the extended BRDF strongly depends on the light incidence angle, the light scattering angle, and the out-of-plane azimuth angle. We observe a 180 degrees symmetry with respect to the azimuth angle. We further investigate the influence of the SSD density, the substrate refractive index, and the statistical distributions of the SSD radius and refractive index on the extended BRDF. For transparent substrates, we also find the dependence of the extended BRDF on the SSD positions.
Physics of a rapid CD4 lymphocyte count with colloidal gold.
Hansen, P; Barry, D; Restell, A; Sylvia, D; Magnin, O; Dombkowski, D; Preffer, F
2012-03-01
The inherent surface charges and small diameters that confer colloidal stability to gold particle conjugates (immunogold) are detrimental to rapid cell surface labeling and distinct cluster definition in flow cytometric light scatter assays. Although the inherent immunogold surface charge prevents self aggregation when stored in liquid suspension, it also slows binding to cells to timeframes of hours and inhibits cell surface coverage. Although the small diameter of immunogold particles prevents settling when in liquid suspension, small particles have small light scattering cross sections and weak light scatter signals. We report a new, small particle lyophilized immunogold reagent that maintains activity after 42°C storage for a year and can be rapidly dissolved into stable liquid suspension for use in labelling cells with larger particle aggregates that have enhanced scattering cross section. Labeling requires less than 1 min at 20°C, which is ∼30 times faster than customary fluorescent antibody labeling. The labeling step involves neutralizing the surface charge of immunogold and creating specifically bound aggregates of gold on the cell surface. This process provides distinct side-scatter cluster separation with blue laser light at 488 nm, which is further improved by using red laser light at 640 nm. Similar comparisons using LED light sources showed less improvement with red light, thereby indicating that coherent light scatter is of significance in enhancing side-scatter cluster separation. The physical principles elucidated here for this technique are compatible with most flow cytometers; however, future studies of its clinical efficacy should be of primary interest in point-of-care applications where robust reagents and rapid results are important. Copyright © 2011 International Society for Advancement of Cytometry.
Index-of-refraction-dependent subcellular light scattering observed with organelle-specific dyes.
Wilson, Jeremy D; Cottrell, William J; Foster, Thomas H
2007-01-01
Angularly resolved light scattering and wavelength-resolved darkfield scattering spectroscopy measurements were performed on intact, control EMT6 cells and cells stained with high-extinction lysosomal- or mitochondrial-localizing dyes. In the presence of the lysosomal-localizing dye NPe6, we observe changes in the details of light scattering from stained and unstained cells, which have both wavelength- and angular-dependent features. Analysis of measurements performed at several wavelengths reveals a reduced scattering cross section near the absorption maximum of the lysosomal-localizing dye. When identical measurements are made with cells loaded with a similar mitochondrial-localizing dye, HPPH, we find no evidence that staining mitochondria had any effect on the light scattering. Changes in the scattering properties of candidate populations of organelles induced by the addition of an absorber are modeled with Mie theory, and we find that any absorber-induced scattering response is very sensitive to the inherent refractive index of the organelle population. Our measurements and modeling are consistent with EMT6-cell-mitochondria having refractive indices close to those reported in the literature for organelles, approximately 1.4. The reduction in scattering cross section induced by NPe6 constrains the refractive index of lysosomes to be significantly higher. We estimate the refractive index of lysosomes in EMT6 cells to be approximately 1.6.
Optical readout of displacements of nanowires along two mutually perpendicular directions
NASA Astrophysics Data System (ADS)
Fu, Chenghua
2017-05-01
Nanowires are good force transducers due to their low mass. The singleness of the direction of the motion detection in a certain system is an existing limitation, and to overcome the limitation is the key point in this article. Optical methods, such as polarized light interferometry and light scattering, are generally used for detecting the displacement of nanowires. Typically, either light interference or light scattering is considered when relating the displacement of a nanowire with the photodetector's measurements. In this work, we consider both the light interference along the optical axis and light scattering perpendicular to the optical axis of a micro-lens fiber optic interferometer. Identifying the displacement along the two directions and the corresponding vibration conversion efficiency coefficients for the nanowire is a significant part of our study. Our analysis shows that the optimal working point of the micro-lens fiber optic interferometer can realize the detection of displacement along the optical axis without the disturbance coming from the motion perpendicular to the optical axis, and vice versa. We use Mie scattering theory to calculate the scattering light for the reason that the size of the nanowire is comparable to the wavelength of light. Our results could provide a guide for optical readout experiments of the displacement of nanowires.
Propagation of laser beams in scattering media.
Zuev, V E; Kabanov, M V; Savelev, B A
1969-01-01
Experimental investigations have been undertaken of some aspects of the propagation of helium-neon gas laser radiation at lambda = 0.63 micro for different scattering media (artificial water fogs, wood smokes, model media). It has been shown that the attenuation coefficients practically coincide when coherent and incoherent radiation is scattered. The applicability limits of Bouguer-Beer's law for describing the attenuation of radiation in scattering media are investigated and the intensity of multiple forward-scattered light for different geometrical parameters of the source and radiation receiver are measured. The applicability of single scattering theory formulas for describing forward-scattered light intensity are discussed.
Spirit Scans Winter Haven (False Color)
NASA Technical Reports Server (NTRS)
2006-01-01
At least three different kinds of rocks await scientific analysis at the place where NASA's Mars Exploration Rover Spirit will likely spend several months of Martian winter. They are visible in this picture, which the panoramic camera on Spirit acquired during the rover's 809th sol, or Martian day, of exploring Mars (April 12, 2006). Paper-thin layers of light-toned, jagged-edged rocks protrude horizontally from beneath small sand drifts; a light gray rock with smooth, rounded edges sits atop the sand drifts; and several dark gray to black, angular rocks with vesicles (small holes) typical of hardened lava lie scattered across the sand. This view is a false-color rendering that combines images taken through the panoramic camera's 753-nanometer, 535-nanometer, and 432-nanometer filters.Optical spectroscopies diagnose cancer
NASA Astrophysics Data System (ADS)
Alfano, Robert R.; Das, Bidyut B.; Glassman, Wenling S.; Pradhan, Asima; Tang, Gui C.
1992-02-01
Today's medical professional is looking beyond the conventional procedures of X-rays, nuclear radiation, magnetic resonance, chemical analysis, and ultrasound to diagnose diseases ranging from cancer to heart ailments. In view of the possible dangerous side effects of X-rays and nuclear radiation, a need exists for novel techniques in disease detection that can either eliminate or reduce their use in examinations. For more than half a century, fluorescence, absorption, and light scattering spectroscopies have been widely used as probes to acquire fundamental knowledge about various physical, chemical, and biological processes. Light may offer alternatives to X-rays and nuclear approaches, and in some cases is non-invasive. Optical spectroscopy and laser technology may offer techniques for the detection and characterization of physical and chemical changes that occur in diseased tissue on a microscopic level.
Probing the Early Universe with the SZ Effect
NASA Technical Reports Server (NTRS)
Joy, M. K.; Carlstrom, J. E.; Rose, M. Franklin (Technical Monitor)
2001-01-01
The Cosmic Microwave Background Radiation (CMBR) which we observe today is relic radiation which last interacted with matter more than 10 billion years ago, when the expanding universe cooled to the point that free electrons and ionized nuclei recombined to form atoms. Prior to recombination, scattering between photons and free electrons was a very frequent occurrence, and the distance light could penetrate was small; afterwards, with free electrons out of circulation, the universe became largely transparent to light. Thus, the CMBR photons we observe today give us a clear view of the state of the early universe. Measured deviations in the intensity of the CMBR trace the small perturbations in the primordial matter density, which have been amplified by gravitational forces to form the magnificent, complex structures which comprise the present-day universe.
Gamma-ray vortices from nonlinear inverse Thomson scattering of circularly polarized light.
Taira, Yoshitaka; Hayakawa, Takehito; Katoh, Masahiro
2017-07-10
Inverse Thomson scattering is a well-known radiation process that produces high-energy photons both in nature and in the laboratory. Nonlinear inverse Thomson scattering occurring inside an intense light field is a process which generates higher harmonic photons. In this paper, we theoretically show that the higher harmonic gamma-ray produced by nonlinear inverse Thomson scattering of circularly polarized light is a gamma-ray vortex, which means that it possesses a helical wave front and carries orbital angular momentum. Our work explains a recent experimental result regarding nonlinear inverse Thomson scattering that clearly shows an annular intensity distribution as a remarkable feature of a vortex beam. Our work implies that gamma-ray vortices should be produced in various situations in astrophysics in which high-energy electrons and intense circularly polarized light fields coexist. Nonlinear inverse Thomson scattering is a promising radiation process for realizing a gamma-ray vortex source based on currently available laser and accelerator technologies, which would be an indispensable tool for exploring gamma-ray vortex science.
High-power laser radiation in atmospheric aerosols: Nonlinear optics of aerodispersed media
NASA Astrophysics Data System (ADS)
Zuev, V. E.; Zemlianov, A. A.; Kopytin, Iu. D.; Kuzikovskii, A. V.
The bulk of this book contains the results of investigations carried out at the Institute of Atmospheric Optics, Siberian Branch, USSR Academy of Science with the participation of the authors. The microphysical and optical characteristics of atmospheric aerosols are considered, taking into account light scattering by a single aerosol particle, light scattering by a system of particles, the scattering phase matrix, light scattering by clouds and fogs, light scattering by hazes, and scattering phase functions of polydispersed aerosols. Other topics studies are related to low-energy (subexplosive) effects of radiation on individual particles, the formation of clear zones in clouds and fogs due to the vaporization of droplets under regular regimes, self-action of a wave beam in a water aerosol under conditions of regular droplet vaporization, laser beam propagation through an explosively evaporating water-droplet aerosol, the propagation of high-power laser radiation through hazes, the ionization and optical breakdown in aerosol media, and laser monitoring of a turbid atmosphere using nonlinear effects.
Evaluation of advanced light scattering technology for microgravity experiments
NASA Technical Reports Server (NTRS)
Fredericks, W. J.; Rosenblum, W. M.
1990-01-01
The capabilities of modern light scattering equipment and the uses it might have in studying processes in microgravity are evaluated. Emphasis is on the resolution of polydisperse systems. This choice was made since a major use of light scattering was expected to be the study of crystal growth of macromolecules in low gravity environments. An evaluation of a modern photon correlation spectrometer and a Mie spectrometer is presented.
NASA Astrophysics Data System (ADS)
Zabarylo, U.; Minet, O.
2010-01-01
Investigations on the application of optical procedures for the diagnosis of rheumatism using scattered light images are only at the beginning both in terms of new image-processing methods and subsequent clinical application. For semi-automatic diagnosis using laser light, the multispectral scattered light images are registered and overlapped to pseudo-coloured images, which depict diagnostically essential contents by visually highlighting pathological changes.
Light Scattering by Marine Particles: Modeling with Non-spherical Shapes
2010-09-30
4271—4282 (1996). Gordon, H.R. and Tao Du, Light scattering by nonspherical particles: application to coccoliths detached from Emiliania huxleyi... Emiliania huxleyi, Applied Optics, (2009). PUBLICATIONS H.R. Gordon, T.J. Smyth, W.M. Balch, and G.C. Boynton, Light scattering by coccoliths...detached from Emiliania huxleyi, Applied Optics, 48, 6059–6073 (2009). [published, refereed] 5 H.R. Gordon, Some Reflections on 35 Years of
Yurinskaya, Valentina; Aksenov, Nikolay; Moshkov, Alexey; Model, Michael; Goryachaya, Tatyana; Vereninov, Alexey
2017-10-01
A decrease in flow cytometric forward light scatter (FSC) is commonly interpreted as a sign of apoptotic cell volume decrease (AVD). However, the intensity of light scattering depends not only on the cell size but also on its other characteristics, such as hydration, which may affect the scattering in the opposite way. That makes estimation of AVD by FSC problematic. Here, we aimed to clarify the relationship between light scattering, cell hydration (assayed by buoyant density) and cell size by the Coulter technique. We used human lymphoid cells U937 exposed to staurosporine, etoposide or hypertonic stress as an apoptotic model. An initial increase in FSC was found to occur in apoptotic cells treated with staurosporine and hypertonic solutions; it is accompanied by cell dehydration and is absent in apoptosis caused by etoposide that is consistent with the lack of dehydration in this case. Thus, the effect of dehydration on the scattering signal outweighs the effect of reduction in cell size. The subsequent FSC decrease, which occurred in parallel to accumulation of annexin-positive cells, was similar in apoptosis caused by all three types of inducers. We conclude that an increase, but not a decrease in light scattering, indicates the initial cell volume decrease associated with apoptotic cell dehydration.
Marcano, Aristides; Alvarado, Salvador; Meng, Junwei; Caballero, Daniel; Moares, Ernesto Marín; Edziah, Raymond
2014-01-01
We developed a pump-probe photothermal lens spectrophotometer that uses a broadband arc-lamp and a set of interference filters to provide tunable, nearly monochromatic radiation between 370 and 730 nm as the pump light source. This light is focused onto an absorbing sample, generating a photothermal lens of millimeter dimensions. A highly collimated monochromatic probe light from a low-power He-Ne laser interrogates the generated lens, yielding a photothermal signal proportional to the absorption of light. We measure the absorption spectra of scattering dye solutions using the device. We show that the spectra are not affected by the presence of scattering, confirming that the method only measures the absorption of light that results in generation of heat. By comparing the photothermal spectra with the usual absorption spectra determined using commercial transmission spectrophotometers, we estimate the quantum yield of scattering of the sample. We discuss applications of the device for spectroscopic characterization of samples such as blood and gold nanoparticles that exhibit a complex behavior upon interaction with light.
LASER BIOLOGY AND MEDICINE: Light scattering study of rheumatoid arthritis
NASA Astrophysics Data System (ADS)
Beuthan, J.; Netz, U.; Minet, O.; Klose, Annerose D.; Hielscher, A. H.; Scheel, A.; Henniger, J.; Müller, G.
2002-11-01
The distribution of light scattered by finger joints is studied in the near-IR region. It is shown that variations in the optical parameters of the tissue (scattering coefficient μs, absorption coefficient μa, and anisotropy factor g) depend on the presence of the rheumatoid arthritis (RA). At the first stage, the distribution of scattered light was measured in diaphanoscopic experiments. The convolution of a Gaussian error function with the scattering phase function proved to be a good approximation of the data obtained. Then, a new method was developed for the reconstruction of distribution of optical parameters in the finger cross section. Model tests of the quality of this reconstruction method show good results.
Luo, Furong; Bao, Xuan; Qin, Yingyan; Hou, Min; Wu, Mingxing
2018-06-01
To evaluate the long-term effect of glistenings and surface light scattering of intraocular lenses (IOLs) on visual and optical performance after cataract surgery. Pseudophakic eyes that underwent standard phacoemulsification and two types of hydrophobic acrylic spherical IOL implantation without complications for at least 5 years were included in this retrospective study. Participants were divided into the glistenings, surface light scattering, and control groups according to the current condition of the IOLs. Then participants received a follow-up examination including uncorrected and corrected distance visual acuity (UDVA and CDVA), contrast sensitivity, straylight, and intraocular higher order aberrations, as well as point spread function (PSF) and modulation transfer function (MTF). A total of 140 eyes were included in the study. UDVA, CDVA, and glare sensitivity were not significantly different among the three groups (P > .05). However, compared with the control group, the IOLs of the glistenings and surface light scattering groups were associated with significantly lower contrast sensitivity under no glare conditions. Furthermore, eye with glistenings exhibited the highest straylight value (P < .05), whereas no difference was found between the surface light scattering and control groups. In contrast to the control group, the spherical aberration increased and the mean values of PSF and MTF decreased in the glistenings and surface light scattering groups. Both glistenings and surface light scattering tend to impair subjective visual performance, such as contrast sensitivity, and potentially affect objective optical quality, including straylight, spherical aberration, PSF, and MTF. [J Refract Surg. 2018;34(6):372-378.]. Copyright 2018, SLACK Incorporated.
Hybrid Monte Carlo-Diffusion Method For Light Propagation in Tissue With a Low-Scattering Region
NASA Astrophysics Data System (ADS)
Hayashi, Toshiyuki; Kashio, Yoshihiko; Okada, Eiji
2003-06-01
The heterogeneity of the tissues in a head, especially the low-scattering cerebrospinal fluid (CSF) layer surrounding the brain has previously been shown to strongly affect light propagation in the brain. The radiosity-diffusion method, in which the light propagation in the CSF layer is assumed to obey the radiosity theory, has been employed to predict the light propagation in head models. Although the CSF layer is assumed to be a nonscattering region in the radiosity-diffusion method, fine arachnoid trabeculae cause faint scattering in the CSF layer in real heads. A novel approach, the hybrid Monte Carlo-diffusion method, is proposed to calculate the head models, including the low-scattering region in which the light propagation does not obey neither the diffusion approximation nor the radiosity theory. The light propagation in the high-scattering region is calculated by means of the diffusion approximation solved by the finite-element method and that in the low-scattering region is predicted by the Monte Carlo method. The intensity and mean time of flight of the detected light for the head model with a low-scattering CSF layer calculated by the hybrid method agreed well with those by the Monte Carlo method, whereas the results calculated by means of the diffusion approximation included considerable error caused by the effect of the CSF layer. In the hybrid method, the time-consuming Monte Carlo calculation is employed only for the thin CSF layer, and hence, the computation time of the hybrid method is dramatically shorter than that of the Monte Carlo method.
Hybrid Monte Carlo-diffusion method for light propagation in tissue with a low-scattering region.
Hayashi, Toshiyuki; Kashio, Yoshihiko; Okada, Eiji
2003-06-01
The heterogeneity of the tissues in a head, especially the low-scattering cerebrospinal fluid (CSF) layer surrounding the brain has previously been shown to strongly affect light propagation in the brain. The radiosity-diffusion method, in which the light propagation in the CSF layer is assumed to obey the radiosity theory, has been employed to predict the light propagation in head models. Although the CSF layer is assumed to be a nonscattering region in the radiosity-diffusion method, fine arachnoid trabeculae cause faint scattering in the CSF layer in real heads. A novel approach, the hybrid Monte Carlo-diffusion method, is proposed to calculate the head models, including the low-scattering region in which the light propagation does not obey neither the diffusion approximation nor the radiosity theory. The light propagation in the high-scattering region is calculated by means of the diffusion approximation solved by the finite-element method and that in the low-scattering region is predicted by the Monte Carlo method. The intensity and mean time of flight of the detected light for the head model with a low-scattering CSF layer calculated by the hybrid method agreed well with those by the Monte Carlo method, whereas the results calculated by means of the diffusion approximation included considerable error caused by the effect of the CSF layer. In the hybrid method, the time-consuming Monte Carlo calculation is employed only for the thin CSF layer, and hence, the computation time of the hybrid method is dramatically shorter than that of the Monte Carlo method.
NASA Technical Reports Server (NTRS)
Sun, W.; Loeb, N. G.; Fu, Q.
2004-01-01
A recently developed finite-difference time domain scheme is examined using the exact analytic solutions for light scattering by a coated sphere immersed in an absorbing medium. The relative differences are less than 1% in the extinction, scattering, and absorption efficiencies and less than 5% in the scattering phase functions. The definition of apparent single-scattering properties is also discussed. (C) 2003 Elsevier Ltd. All rights reserved.
Comparative Study of Light Scattering from Hepatoma Cells and Hepatocytes
NASA Astrophysics Data System (ADS)
Lin, Xiaogang; Wang, Rongrong; Guo, Yongcai; Gao, Chao; Guo, Xiaoen
2012-11-01
Primary liver cancer is one of the highest mortality malignant tumors in the world. China is a high occurrence area of primary liver cancer. Diagnosis of liver cancer, especially early diagnosis, is essential for improving patients' survival. Light scattering and measuring method is an emerging technology developed in recent decades, which has attracted a large number of biomedical researchers due to its advantages, such as fast, simple, high accuracy, good repeatability, and non-destructive. The hypothesis of this project is that there may be some different light scattering information between hepatoma cells and hepatocyte. Combined with the advantages of the dynamic light scattering method and the biological cytology, an experimental scheme to measure the light scattering information of cells was formulated. Hepatoma cells and hepatic cells were irradiated by a semiconductor laser (532 nm). And the Brookhaven BI-200SM wide-angle light scattering device and temperature control apparatus were adopted. The light scattering information of hepatoma cells and hepatic cells in vitro within the 15°C to 30°C temperature range was processed by a BI-9000AT digital autocorrelator. The following points were found: (a) the scattering intensities of human hepatic cells and hepatoma cells are nearly not affected by the temperature factor, and the former is always greater than the latter and (b) the relaxation time of hepatoma cells is longer than that of hepatic cells, and both the relaxation time are shortened with increasing temperature from 15°C to 25°C. It can be concluded that hepatoma cells could absorb more incident light than hepatic cells. The reason may be that there exists more protein and nucleic acid in cancerous cells than normal cells. Furthermore, based on the length relaxation time, a conclusion can be inferred that the Brownian movement of cancer cells is greater.
Broadband optical switch based on liquid crystal dynamic scattering.
Geis, M W; Bos, P J; Liberman, V; Rothschild, M
2016-06-27
This work demonstrates a novel broadband optical switch, based on dynamic-scattering effect in liquid crystals (LCs). Dynamic-scattering-mode technology was developed for display applications over four decades ago, but was displaced in favor of the twisted-nematic LCs. However, with the recent development of more stable LCs, dynamic scattering provides advantages over other technologies for optical switching. We demonstrate broadband polarization-insensitive attenuation of light directly passing thought the cell by 4 to 5 orders of magnitude at 633 nm. The attenuation is accomplished by light scattering to higher angles. Switching times of 150 μs to 10% transmission have been demonstrated. No degradation of devices is found after hundreds of switching cycles. The light-rejection mechanism is due to scattering, induced by disruption of LC director orientation with dopant ion motion with an applied electric field. Angular dependence of scattering is characterized as a function of bias voltage.
NASA Technical Reports Server (NTRS)
Piatek, J. L.; Hapke, B. W.; Nelson, R. M.; Hale, A. S.; Smythe, W. D.
2003-01-01
The nature of the scattering of light is thought to be well understood when the medium is made up of independent scatterers that are much larger than the wavelength of that light. This is not the case when the size of the scattering objects is similar to or smaller than the wavelength or the scatterers are not independent. In an attempt to examine the applicability of independent particle scattering models, to planetary regoliths, a dataset of experimental results were compared with theoretical predictions.
A nanoporous gold membrane for sensing applications
Oo, Swe Zin; Silva, Gloria; Carpignano, Francesca; Noual, Adnane; Pechstedt, Katrin; Mateos, Luis; Grant-Jacob, James A.; Brocklesby, Bill; Horak, Peter; Charlton, Martin; Boden, Stuart A.; Melvin, Tracy
2016-01-01
Design and fabrication of three-dimensionally structured, gold membranes containing hexagonally close-packed microcavities with nanopores in the base, are described. Our aim is to create a nanoporous structure with localized enhancement of the fluorescence or Raman scattering at, and in the nanopore when excited with light of approximately 600 nm, with a view to provide sensitive detection of biomolecules. A range of geometries of the nanopore integrated into hexagonally close-packed assemblies of gold micro-cavities was first evaluated theoretically. The optimal size and shape of the nanopore in a single microcavity were then considered to provide the highest localized plasmon enhancement (of fluorescence or Raman scattering) at the very center of the nanopore for a bioanalyte traversing through. The optimized design was established to be a 1200 nm diameter cavity of 600 nm depth with a 50 nm square nanopore with rounded corners in the base. A gold 3D-structured membrane containing these sized microcavities with the integrated nanopore was successfully fabricated and ‘proof of concept’ Raman scattering experiments are described. PMID:26973809
Efficient Light Extraction from Organic Light-Emitting Diodes Using Plasmonic Scattering Layers
DOE Office of Scientific and Technical Information (OSTI.GOV)
Rothberg, Lewis
2012-11-30
Our project addressed the DOE MYPP 2020 goal to improve light extraction from organic light-emitting diodes (OLEDs) to 75% (Core task 6.3). As noted in the 2010 MYPP, “the greatest opportunity for improvement is in the extraction of light from [OLED] panels”. There are many approaches to avoiding waveguiding limitations intrinsic to the planar OLED structure including use of textured substrates, microcavity designs and incorporating scattering layers into the device structure. We have chosen to pursue scattering layers since it addresses the largest source of loss which is waveguiding in the OLED itself. Scattering layers also have the potential tomore » be relatively robust to color, polarization and angular distributions. We note that this can be combined with textured or microlens decorated substrates to achieve additional enhancement.« less
Light Scattering by Marine Particles: Modeling with Non-spherical Shapes
2008-01-01
Emiliania huxleyi, Limnology and Oceanography, 46, 1438−1454. Gordon, H.R., 2004, Inverse Radiative Transfer, Coccolith Backscattering, and Light Scattering...16430. Voss, K.J., W.M. Balch, and K.A. Kilpatrick, 1998, Scattering and attenuation properties of Emiliania huxleyi cells and their detached
Lighting design for globally illuminated volume rendering.
Zhang, Yubo; Ma, Kwan-Liu
2013-12-01
With the evolution of graphics hardware, high quality global illumination becomes available for real-time volume rendering. Compared to local illumination, global illumination can produce realistic shading effects which are closer to real world scenes, and has proven useful for enhancing volume data visualization to enable better depth and shape perception. However, setting up optimal lighting could be a nontrivial task for average users. There were lighting design works for volume visualization but they did not consider global light transportation. In this paper, we present a lighting design method for volume visualization employing global illumination. The resulting system takes into account view and transfer-function dependent content of the volume data to automatically generate an optimized three-point lighting environment. Our method fully exploits the back light which is not used by previous volume visualization systems. By also including global shadow and multiple scattering, our lighting system can effectively enhance the depth and shape perception of volumetric features of interest. In addition, we propose an automatic tone mapping operator which recovers visual details from overexposed areas while maintaining sufficient contrast in the dark areas. We show that our method is effective for visualizing volume datasets with complex structures. The structural information is more clearly and correctly presented under the automatically generated light sources.
Polarized bow shocks reveal features of the winds and environments of massive stars
NASA Astrophysics Data System (ADS)
Shrestha, Manisha
2018-01-01
Massive stars strongly affect their surroundings through their energetic stellar winds and deaths as supernovae. The bow shock structures created by fast-moving massive stars contain important information about the winds and ultimate fates of these stars as well as their local interstellar medium (ISM). Since bow shocks are aspherical, the light scattered in the dense shock material becomes polarized. Analyzing this polarization reveals details of the bow shock geometry as well as the composition, velocity, density, and albedo of the scattering material. With these quantities, we can constrain the properties of the stellar wind and thus the evolutionary state of the star, as well as the dust composition of the local ISM.In my dissertation research, I use a Monte Carlo radiative transfer code that I optimized to simulate the polarization signatures produced by both resolved and unresolved stellar wind bow shocks (SWBS) illuminated by a central star and by shock emission. I derive bow shock shapes and densities from published analytical calculations and smooth particle hydrodynamic (SPH) models. In the case of the analytical SWBS and electron scattering, I find that higher optical depths produce higher polarization and position angle rotations at specific viewing angles compared to theoretical predictions for low optical depths. This is due to the geometrical properties of the bow shock combined with multiple scattering effects. For dust scattering, the polarization signature is strongly affected by wavelength, dust grain properties, and viewing angle. The behavior of the polarization as a function of wavelength in these cases can distinguish among different dust models for the local ISM. In the case of SPH density structures, I investigate how the polarization changes as a function of the evolutionary phase of the SWBS. My dissertation compares these simulations with polarization data from Betelgeuse and other massive stars with bow shocks. I discuss the implications of these model for the stellar winds and interstellar environments of these influential objects.
First Scattered-light Images of the Gas-rich Debris Disk around 49 Ceti
DOE Office of Scientific and Technical Information (OSTI.GOV)
Choquet, Élodie; Milli, Julien; Wahhaj, Zahed
We present the first scattered-light images of the debris disk around 49 Ceti, a ∼40 Myr A1 main-sequence star at 59 pc, famous for hosting two massive dust belts as well as large quantities of atomic and molecular gas. The outer disk is revealed in reprocessed archival Hubble Space Telescope NICMOS-F110W images, as well as new coronagraphic H-band images from the Very Large Telescope SPHERE instrument. The disk extends from 1.″1 (65 au) to 4.″6 (250 au) and is seen at an inclination of 73°, which refines previous measurements at lower angular resolution. We also report no companion detection largermore » than 3 M {sub Jup} at projected separations beyond 20 au from the star (0.″34). Comparison between the F110W and H-band images is consistent with a gray color of 49 Ceti’s dust, indicating grains larger than ≳2 μ m. Our photometric measurements indicate a scattering efficiency/infrared excess ratio of 0.2–0.4, relatively low compared to other characterized debris disks. We find that 49 Ceti presents morphological and scattering properties very similar to the gas-rich HD 131835 system. From our constraint on the disk inclination we find that the atomic gas previously detected in absorption must extend to the inner disk, and that the latter must be depleted of CO gas. Building on previous studies, we propose a schematic view of the system describing the dust and gas structure around 49 Ceti and hypothetical scenarios for the gas nature and origin.« less
Polarized Optical Scattering Measurements of Metallic Nanoparticles on a Thin Film Silicon Wafer
NASA Astrophysics Data System (ADS)
Liu, Cheng-Yang; Liu, Tze-An; Fu, Wei-En
2009-09-01
Light scattering has shown its powerful diagnostic capability to characterize optical quality surfaces. In this study, the theory of bidirectional reflectance distribution function (BRDF) was used to analyze the metallic nanoparticles' sizes on wafer surfaces. The BRDF of a surface is defined as the angular distribution of radiance scattered by the surface normalized by the irradiance incident on the surface. A goniometric optical scatter instrument has been developed to perform the BRDF measurements on polarized light scattering on wafer surfaces for the diameter and distribution measurements of metallic nanoparticles. The designed optical scatter instrument is capable of distinguishing various types of optical scattering characteristics, which are corresponding to the diameters of the metallic nanoparticles, near surfaces by using the Mueller matrix calculation. The metallic nanoparticle diameter of measurement is 60 nm on 2 inch thin film wafers. These measurement results demonstrate that the polarization of light scattered by metallic particles can be used to determine the size of metallic nanoparticles on silicon wafers.
García, Antonio A.; Pirez-Gomez, Miguel A.; Pech-Pacheco, José L.; Mendez-Galvan, Jorge F.; Machain-Williams, Carlos; Talavera-Aguilar, Lourdes; Espinosa-Carrillo, José H.; Duarte-Villaseñor, Miriam M.; Be-Ortiz, Christian; Espinosa-de los Monteros, Luz E.; Castillo-Pacheco, Ariel; Garcia-Rejon, Julian E.
2017-01-01
Antibody detection and accurate diagnosis of tropical diseases is essential to help prevent the spread of disease. However, most detection methods lack cost-effectiveness and field portability, which are essential features for achieving diagnosis in a timely manner. To address this, 3D-printed oblate spheroid sample chambers were fabricated to measure green light scattering of gold nanoparticles using an optical caustic focus to detect antibodies. Scattering signals of 20–200 nm gold nanoparticles using a green laser were compared to green light emitting diode (LED) light source signals and to Mie theory. The change in signal from 60 to 120 nm decreased in the order of Mie Theory > optical caustic scattering > 90° scattering. These results suggested that conjugating 60 nm gold nanoparticles and using an optical caustic system to detect plasmonic light scattering, would result in a sensitive test for detecting human antibodies in serum. Therefore, we studied the light scattering response of conjugated gold nanoparticles exposed to different concentrations of anti-protein E antibody, and a feasibility study of 10 human serum samples using dot blot and a handheld optical caustic-based sensor device. The overall agreement between detection methods suggests that the new sensor concept shows promise to detect gold nanoparticle aggregation in a homogeneous assay. Further testing and protocol optimization is needed to draw conclusions on the positive and negative predictive values for this new testing system. PMID:28817080
Soliton-induced relativistic-scattering and amplification.
Rubino, E; Lotti, A; Belgiorno, F; Cacciatori, S L; Couairon, A; Leonhardt, U; Faccio, D
2012-01-01
Solitons are of fundamental importance in photonics due to applications in optical data transmission and also as a tool for investigating novel phenomena ranging from light generation at new frequencies and wave-trapping to rogue waves. Solitons are also moving scatterers: they generate refractive index perturbations moving at the speed of light. Here we found that such perturbations scatter light in an unusual way: they amplify light by the mixing of positive and negative frequencies, as we describe using a first Born approximation and numerical simulations. The simplest scenario in which these effects may be observed is within the initial stages of optical soliton propagation: a steep shock front develops that may efficiently scatter a second, weaker probe pulse into relatively intense positive and negative frequency modes with amplification at the expense of the soliton. Our results show a novel all-optical amplification scheme that relies on soliton induced scattering.
Light atom quantum oscillations in UC and US
Yiu, Yuen; Aczel, Adam A.; Granroth, Garrett E.; ...
2016-01-19
High energy vibrational scattering in the binary systems UC and US is measured using time-of-flight inelastic neutron scattering. A clear set of well-defined peaks equally separated in energy is observed in UC, corresponding to harmonic oscillations of the light C atoms in a cage of heavy U atoms. The scattering is much weaker in US and only a few oscillator peaks are visible. We show how the difference between the materials can be understood by considering the neutron scattering lengths and masses of the lighter atoms. Monte Carlo ray tracing is used to simulate the scattering, with near quantitative agreementmore » with the data in UC, and some differences with US. The possibility of observing anharmonicity and anisotropy in the potentials of the light atoms is investigated in UC. Lastly, the observed data is well accounted for by considering each light atom as a single atom isotropic quantum harmonic oscillator.« less
Magnon and phonon thermometry with inelastic light scattering
NASA Astrophysics Data System (ADS)
Olsson, Kevin S.; An, Kyongmo; Li, Xiaoqin
2018-04-01
Spin caloritronics investigates the interplay between the transport of spin and heat. In the spin Seebeck effect, a thermal gradient across a magnetic material generates a spin current. A temperature difference between the energy carriers of the spin and lattice subsystems, namely the magnons and phonons, is necessary for such thermal nonequilibrium generation of spin current. Inelastic light scattering is a powerful method that can resolve the individual temperatures of magnons and phonons. In this review, we discuss the thermometry capabilities of inelastic light scattering for measuring optical and acoustic phonons, as well as magnons. A scattering spectrum offers three temperature sensitive parameters: frequency shift, linewidth, and integrated intensity. We discuss the temperatures measured via each of these parameters for both phonon and magnons. Finally, we discuss inelastic light scattering experiments that have examined the magnon and phonon temperatures in thermal nonequilibrium which are particularly relevant to spin caloritronic phenomena.
Ultrasonic trap for light scattering measurement
NASA Astrophysics Data System (ADS)
Barton, Petr; Pavlu, Jiri
2017-04-01
Light scattering is complex phenomenon occurring widely in space environments, including the dense dusty clouds, nebulas or even the upper atmosphere of the Earth. However, when the size of the dust (or of other scattering center) is close to the incident light wavelength, theoretical determination is difficult. In such case, Mie theory is to be used but there is a lack of the material constants for most space-related materials. For experimental measurement of light scattering, we designed unique apparatus, based on ultrasonic trap. Using acoustic levitation we are able to capture the dust grain in midair, irradiate it with laser, and observe scattering directly with goniometer-mounted photodiode. Advantage of this approach is ability to measure directly in the air (thus, no need for the carrier medium) and possibility to study non-spherical particles. Since the trap development is nearly finished and initial experiments are carried out, the paper presents first tests on water droplets.
Study of coherent reflectometer for imaging internal structures of highly scattering media
NASA Astrophysics Data System (ADS)
Poupardin, Mathieu; Dolfi, Agnes
1996-01-01
Optical reflectometers are potentially useful tools for imaging internal structures of turbid media, particularly of biological media. To get a point by point image, an active imaging system has to distinguish light scattered from a sample volume and light scattered by other locations in the media. Operating this discrimination of light with reflectometers based on coherence can be realized in two ways: assuring a geometric selection or a temporal selection. In this paper we present both methods, showing in each case the influence of the different parameters on the size of the sample volume under the assumption of single scattering. We also study the influence on the detection efficiency of the coherence loss of the incident light resulting from multiple scattering. We adapt a model, first developed for atmospheric lidar in turbulent atmosphere, to get an analytical expression of this detection efficiency in the function of the optical coefficients of the media.
Israelsen, Nathan D; Wooley, Donald; Hanson, Cynthia; Vargis, Elizabeth
2016-01-01
Surface-enhanced Raman scattering (SERS) is a powerful light scattering technique that can be used for sensitive immunoassay development and cell labeling. A major obstacle to using SERS is the complexity of fabricating SERS probes since they require nanoscale characterization and optical uniformity. The light scattering response of SERS probes may also be modulated by the substrate used for SERS analysis. A typical SERS substrate such as quartz can be expensive. Polystyrene is a cheaper substrate option but can decrease the SERS response due to interfering Raman emission peaks and high background fluorescence. The goal of this research is to develop an optimized process for fabricating Raman-labeled nanoparticles for a SERS-based immunoassay on a polystyrene substrate. We have developed a method for fabricating SERS nanoparticle probes for use in a light scattering immunoassay on a polystyrene substrate. The light scattering profile of both spherical gold nanoparticle and gold nanorod SERS probes were characterized using Raman spectroscopy and optical absorbance spectroscopy. The effects of substrate interference and autofluorescence were reduced by selecting a Raman reporter with a strong light scattering response in a spectral region where interfering substrate emission peaks are minimized. Both spherical gold nanoparticles and gold nanorods SERS probes used in the immunoassay were detected at labeling concentrations in the low pM range. This analytical sensitivity falls within the typical dynamic range for direct labeling of cell-surface biomarkers using SERS probes. SERS nanoparticle probes were fabricated to produce a strong light scattering signal despite substrate interference. The optical extinction and inelastic light scattering of these probes was detected by optical absorbance spectroscopy and Raman spectroscopy, respectively. This immunoassay demonstrates the feasibility of analyzing strongly enhanced Raman signals on polystyrene, which is an inexpensive yet non-ideal Raman substrate. The assay sensitivity, which is in the low pM range, suggests that these SERS probe particles could be used for Raman labeling of cell or tissue samples in a polystyrene tissue culture plate. With continued development, this approach could be used for direct labeling of multiple cell surface biomarkers on strongly interfering substrate platforms.
NASA Technical Reports Server (NTRS)
Grams, G. W.
1982-01-01
A variety of studies were carried out to help establish the accuracy of quantities describing physical characteristics of cloud particles (such as size, shape, and composition) that are to be inferred from light scattering data obtained with the nephelameter experiment on the Galileo spacecraft. The objectives were to provide data for validating and testing procedures for analyzing the Galileo nephelameter data with light scattering observations in a variety of on-going laboratory and field measurement programs for which simultaneous observations of the physical characteristics of the scattering particles were available.
Filamentation of ultrashort light pulses in a liquid scattering medium
NASA Astrophysics Data System (ADS)
Jukna, V.; Tamošauskas, G.; Valiulis, G.; Aputis, M.; Puida, M.; Ivanauskas, F.; Dubietis, A.
2009-01-01
We have studied filamentation of 1-ps laser pulses in a scattering medium (aqueous suspension of 2-μm polystyrene microspheres) and compared filamentation dynamics to that in pure water. Our results indicate that light scattering does not alter filamentation dynamics in general, but rather results in farther position of the nonlinear focus, shorter filament length, and the development of speckle structure in the peripheral part of the beam. The experimental observations are qualitatively reproduced by the numerical model which accounts for diffraction, self-focusing, multiphoton absorption, and light scattering introduced through a stochastic diffusion and diffraction term.
Backward elastic light scattering of malaria infected red blood cells
NASA Astrophysics Data System (ADS)
Lee, Seungjun; Lu, Wei
2011-08-01
We investigated the backward light scattering pattern of healthy and malaria (Plasmodium falciparum) parasitized red blood cells. The spectrum could clearly distinguish between predominant ring stage infected blood cells and healthy blood cells. Further, we found that infected samples mixed with different stages of P. falciparum showed different signals, suggesting that even variance in parasite stages could also be detected by the spectrum. These results together with the backward scattering technique suggest the potential of non-invasive diagnosis of malaria through light scattering of blood cells near the surface of human body, such as using eyes or skin surface.
Absorption and scattering of light by nonspherical particles. [in atmosphere
NASA Technical Reports Server (NTRS)
Bohren, C. F.
1986-01-01
Using the example of the polarization of scattered light, it is shown that the scattering matrices for identical, randomly ordered particles and for spherical particles are unequal. The spherical assumptions of Mie theory are therefore inconsistent with the random shapes and sizes of atmospheric particulates. The implications for corrections made to extinction measurements of forward scattering light are discussed. Several analytical methods are examined as potential bases for developing more accurate models, including Rayleigh theory, Fraunhoffer Diffraction theory, anomalous diffraction theory, Rayleigh-Gans theory, the separation of variables technique, the Purcell-Pennypacker method, the T-matrix method, and finite difference calculations.
Double scattering of light from Biophotonic Nanostructures with short-range order
DOE Office of Scientific and Technical Information (OSTI.GOV)
Noh, Heeso; Liew, Seng Fatt; Saranathan, Vinodkumar
2010-07-28
We investigate the physical mechanism for color production by isotropic nanostructures with short-range order in bird feather barbs. While the primary peak in optical scattering spectra results from constructive interference of singly-scattered light, many species exhibit secondary peaks with distinct characteristic. Our experimental and numerical studies show that these secondary peaks result from double scattering of light by the correlated structures. Without an analog in periodic or random structures, such a phenomenon is unique for short-range ordered structures, and has been widely used by nature for non-iridescent structural coloration.
Atomic Dynamics in Simple Liquid: de Gennes Narrowing Revisited
Wu, Bin; Iwashita, Takuya; Egami, Takeshi
2018-03-27
The de Gennes narrowing phenomenon is frequently observed by neutron or x-ray scattering measurements of the dynamics of complex systems, such as liquids, proteins, colloids, and polymers. The characteristic slowing down of dynamics in the vicinity of the maximum of the total scattering intensity is commonly attributed to enhanced cooperativity. In this Letter, we present an alternative view on its origin through the examination of the time-dependent pair correlation function, the van Hove correlation function, for a model liquid in two, three, and four dimensions. We find that the relaxation time increases monotonically with distance and the dependence on distancemore » varies with dimension. We propose a heuristic explanation of this dependence based on a simple geometrical model. Furthermore, this finding sheds new light on the interpretation of the de Gennes narrowing phenomenon and the α-relaxation time.« less
Contamination detection NDE for cleaning process inspection
NASA Technical Reports Server (NTRS)
Marinelli, W. J.; Dicristina, V.; Sonnenfroh, D.; Blair, D.
1995-01-01
In the joining of multilayer materials, and in welding, the cleanliness of the joining surface may play a large role in the quality of the resulting bond. No non-intrusive techniques are currently available for the rapid measurement of contamination on large or irregularly shaped structures prior to the joining process. An innovative technique for the measurement of contaminant levels in these structures using laser based imaging is presented. The approach uses an ultraviolet excimer laser to illuminate large and/or irregular surface areas. The UV light induces fluorescence and is scattered from the contaminants. The illuminated area is viewed by an image-intensified CCD (charge coupled device) camera interfaced to a PC-based computer. The camera measures the fluorescence and/or scattering from the contaminants for comparison with established standards. Single shot measurements of contamination levels are possible. Hence, the technique may be used for on-line NDE testing during manufacturing processes.
Atomic Dynamics in Simple Liquid: de Gennes Narrowing Revisited
DOE Office of Scientific and Technical Information (OSTI.GOV)
Wu, Bin; Iwashita, Takuya; Egami, Takeshi
The de Gennes narrowing phenomenon is frequently observed by neutron or x-ray scattering measurements of the dynamics of complex systems, such as liquids, proteins, colloids, and polymers. The characteristic slowing down of dynamics in the vicinity of the maximum of the total scattering intensity is commonly attributed to enhanced cooperativity. In this Letter, we present an alternative view on its origin through the examination of the time-dependent pair correlation function, the van Hove correlation function, for a model liquid in two, three, and four dimensions. We find that the relaxation time increases monotonically with distance and the dependence on distancemore » varies with dimension. We propose a heuristic explanation of this dependence based on a simple geometrical model. Furthermore, this finding sheds new light on the interpretation of the de Gennes narrowing phenomenon and the α-relaxation time.« less
Feedback mechanism for smart nozzles and nebulizers
Montaser, Akbar [Potomac, MD; Jorabchi, Kaveh [Arlington, VA; Kahen, Kaveh [Kleinburg, CA
2009-01-27
Nozzles and nebulizers able to produce aerosol with optimum and reproducible quality based on feedback information obtained using laser imaging techniques. Two laser-based imaging techniques based on particle image velocimetry (PTV) and optical patternation map and contrast size and velocity distributions for indirect and direct pneumatic nebulizations in plasma spectrometry. Two pulses from thin laser sheet with known time difference illuminate droplets flow field. Charge coupled device (CCL)) captures scattering of laser light from droplets, providing two instantaneous particle images. Pointwise cross-correlation of corresponding images yields two-dimensional velocity map of aerosol velocity field. For droplet size distribution studies, solution is doped with fluorescent dye and both laser induced florescence (LIF) and Mie scattering images are captured simultaneously by two CCDs with the same field of view. Ratio of LIF/Mie images provides relative droplet size information, then scaled by point calibration method via phase Doppler particle analyzer.
Jacobson, C M; Borchardt, M T; Den Hartog, D J; Falkowski, A F; Morton, L A; Thomas, M A
2016-11-01
The Thomson scattering diagnostic on the Madison Symmetric Torus (MST) records excessive levels of stray Nd:YAG laser light. Stray light saturates the 1064 nm spectral channel in all polychromators, which prevents absolute electron density measurements via Rayleigh scattering calibration. Furthermore, stray light contaminates adjacent spectral channels for r/a ≥ 0.75, which renders the diagnostic unable to make electron temperature measurements at these radii. In situ measurements of stray light levels during a vacuum vessel vent are used to identify stray light sources and strategies for reduction of stray light levels. Numerical modeling using Zemax OpticStudio supports these measurements. The model of the vacuum vessel and diagnostic includes synthetic collection optics to enable direct comparison of measured and simulated stray light levels. Modeling produces qualitatively similar stray light distributions to MST measurements, and quantifies the mitigation effects of stray light mitigation strategies prior to implementation.
NASA Astrophysics Data System (ADS)
Jacobson, C. M.; Borchardt, M. T.; Den Hartog, D. J.; Falkowski, A. F.; Morton, L. A.; Thomas, M. A.
2016-11-01
The Thomson scattering diagnostic on the Madison Symmetric Torus (MST) records excessive levels of stray Nd:YAG laser light. Stray light saturates the 1064 nm spectral channel in all polychromators, which prevents absolute electron density measurements via Rayleigh scattering calibration. Furthermore, stray light contaminates adjacent spectral channels for r/a ≥ 0.75, which renders the diagnostic unable to make electron temperature measurements at these radii. In situ measurements of stray light levels during a vacuum vessel vent are used to identify stray light sources and strategies for reduction of stray light levels. Numerical modeling using Zemax OpticStudio supports these measurements. The model of the vacuum vessel and diagnostic includes synthetic collection optics to enable direct comparison of measured and simulated stray light levels. Modeling produces qualitatively similar stray light distributions to MST measurements, and quantifies the mitigation effects of stray light mitigation strategies prior to implementation.
Observation of cooperative Mie scattering from an ultracold atomic cloud
DOE Office of Scientific and Technical Information (OSTI.GOV)
Bender, H.; Stehle, C.; Slama, S.
Scattering of light at a distribution of scatterers is an intrinsically cooperative process, which means that the scattering rate and the angular distribution of the scattered light are essentially governed by bulk properties of the distribution, such as its size, shape, and density, although local disorder and density fluctuations may have an important impact on the cooperativity. Via measurements of the radiation pressure force exerted by a far-detuned laser beam on a very small and dense cloud of ultracold atoms, we are able to identify the respective roles of superradiant acceleration of the scattering rate and of Mie scattering inmore » the cooperative process. They lead, respectively, to a suppression or an enhancement of the radiation pressure force. We observe a maximum in the radiation pressure force as a function of the phase shift induced in the incident laser beam by the cloud's refractive index. The maximum marks the borderline of the validity of the Rayleigh-Debye-Gans approximation from a regime, where Mie scattering is more complex. Our observations thus help to clarify the intricate relationship between Rayleigh scattering of light at a coarse-grained ensemble of individual scatterers and Mie scattering at the bulk density distribution.« less
DOE Office of Scientific and Technical Information (OSTI.GOV)
Xiong, Ming; Yang, Liping; Liu, Ying D.
Interplanetary corotating interaction regions (CIRs) can be remotely imaged in white light (WL), as demonstrated by the Solar Mass Ejection Imager (SMEI) on board the Coriolis spacecraft and Heliospheric Imagers (HIs) on board the twin Solar TErrestrial RElations Observatory ( STEREO ) spacecraft. The interplanetary WL intensity, due to Thomson scattering of incident sunlight by free electrons, is jointly determined by the 3D distribution of electron number density and line-of-sight (LOS) weighting factors of the Thomson-scattering geometry. The 2D radiance patterns of CIRs in WL sky maps look very different from different 3D viewpoints. Because of the in-ecliptic locations ofmore » both the STEREO and Coriolis spacecraft, the longitudinal dimension of interplanetary CIRs has, up to now, always been integrated in WL imagery. To synthesize the WL radiance patterns of CIRs from an out-of-ecliptic (OOE) vantage point, we perform forward magnetohydrodynamic modeling of the 3D inner heliosphere during Carrington Rotation CR1967 at solar maximum. The mixing effects associated with viewing 3D CIRs are significantly minimized from an OOE viewpoint. Our forward modeling results demonstrate that OOE WL imaging from a latitude greater than 60° can (1) enable the garden-hose spiral morphology of CIRs to be readily resolved, (2) enable multiple coexisting CIRs to be differentiated, and (3) enable the continuous tracing of any interplanetary CIR back toward its coronal source. In particular, an OOE view in WL can reveal where nascent CIRs are formed in the extended corona and how these CIRs develop in interplanetary space. Therefore, a panoramic view from a suite of wide-field WL imagers in a solar polar orbit would be invaluable in unambiguously resolving the large-scale longitudinal structure of CIRs in the 3D inner heliosphere.« less
NASA Astrophysics Data System (ADS)
Silva, F. M. L.; Alencar, L. D. S.; Bernardi, M. I. B.; Lima, F. W. S.; Melo, C. A. S.
2015-06-01
In this work we investigate the scattering of light in means turbid in the presence or not of pigment and nanoparticles. For this we initially using a sample of collagen from means turbid with and without the presence of curcuma pigments and nanoparticles. Our results show that the light scattering is more intense in the samples with nanoparticles and curcuma pigment.
A review and reassessment of diffraction, scattering, and shadows in electrodynamics
NASA Astrophysics Data System (ADS)
Berg, Matthew J.; Sorensen, Christopher M.
2018-05-01
The concepts of diffraction and scattering are well known and considered fundamental in optics and other wave phenomena. For any type of wave, one way to define diffraction is the spreading of waves, i.e., no change in the average propagation direction, while scattering is the deflection of waves with a clear change of propagation direction. However, the terms "diffraction" and "scattering" are often used interchangeably, and hence, a clear distinction between the two is difficult to find. This review considers electromagnetic waves and retains the simple definition that diffraction is the spreading of waves but demonstrates that all diffraction patterns are the result of scattering. It is shown that for electromagnetic waves, the "diffracted" wave from an object is the Ewald-Oseen extinction wave in the far-field zone. The intensity distribution of this wave yields what is commonly called the diffraction pattern. Moreover, this is the same Ewald-Oseen wave that cancels the incident wave inside the object and thereafter continues to do so immediately behind the object to create a shadow. If the object is much wider than the beam but has a hole, e.g., a screen with an aperture, the Ewald-Oseen extinction wave creates the shadow behind the screen and the incident light that passes through the aperture creates the diffraction pattern. This point of view also illustrates Babinet's principle. Thus, it is the Ewald-Oseen extinction theorem that binds together diffraction, scattering, and shadows.
A preview of a modular surface light scattering instrument with autotracking optics
NASA Technical Reports Server (NTRS)
Meyer, William V.; Tin, Padetha; Mann, J. Adin, Jr.; Cheung, H. Michael; Rogers, Richard B.; Lading, Lars
1994-01-01
NASA's Advanced Technology Development (ATD) program is sponsoring the development of a new generation of surface light scattering hardware. This instrument is designed to non-invasively measure the surface response function of liquids over a wide range of operating conditions while automatically compensating for a sloshing surface. The surface response function can be used to compute surface tension, properties of monolayers present, viscosity, surface tension gradient and surface temperature. The instrument uses optical and electronic building blocks developed for the laser light scattering program at NASA Lewis along with several unique surface light scattering components. The emphasis of this paper is the compensation for bulk surface motion (slosh). Some data processing background information is also included.
Riesner, Detlev; Buenemann, Hans
1973-01-01
A stopped-flow apparatus utilizing light-scattering for following the progress of a reaction is described. The method is applicable to all reactions that result in a significant change of the average molecular weight. It was possible due to several modifications of a conventional stopped-flow system to obtain a sensitivity comparable to that of commercial instruments for static light-scattering measurements. Experiments on three reactions are reported: association and dissociation of mercury ligands with DNA, dissociation of the dimers of DNA-dependent RNA polymerase, and complex formation of tRNASer (yeast) with the cognate aminoacyl-tRNA synthetase. The changes in the intensities of the scattered light are calculated and compared with the measured amplitudes. PMID:4577138
NASA Astrophysics Data System (ADS)
Vaudelle, Fabrice; L'Huillier, Jean-Pierre; Askoura, Mohamed Lamine
2017-06-01
Red and near-Infrared light is often used as a useful diagnostic and imaging probe for highly scattering media such as biological tissues, fruits and vegetables. Part of diffusively reflected light gives interesting information related to the tissue subsurface, whereas light recorded at further distances may probe deeper into the interrogated turbid tissues. However, modelling diffusive events occurring at short source-detector distances requires to consider both the distribution of the light sources and the scattering phase functions. In this report, a modified Monte Carlo model is used to compute light transport in curved and multi-layered tissue samples which are covered with a thin and highly diffusing tissue layer. Different light source distributions (ballistic, diffuse or Lambertian) are tested with specific scattering phase functions (modified or not modified Henyey-Greenstein, Gegenbauer and Mie) to compute the amount of backscattered and transmitted light in apple and human skin structures. Comparisons between simulation results and experiments carried out with a multispectral imaging setup confirm the soundness of the theoretical strategy and may explain the role of the skin on light transport in whole and half-cut apples. Other computational results show that a Lambertian source distribution combined with a Henyey-Greenstein phase function provides a higher photon density in the stratum corneum than in the upper dermis layer. Furthermore, it is also shown that the scattering phase function may affect the shape and the magnitude of the Bidirectional Reflectance Distribution (BRDF) exhibited at the skin surface.
Visible light scatter measurements of the Advanced X-ray Astronomical Facility /AXAF/ mirror samples
NASA Technical Reports Server (NTRS)
Griner, D. B.
1981-01-01
NASA is studying the properties of mirror surfaces for X-ray telescopes, the data of which will be used to develop the telescope system for the Advanced X-ray Astronomical Facility. Visible light scatter measurements, using a computer controlled scanner, are made of various mirror samples to determine surface roughness. Total diffuse scatter is calculated using numerical integration techniques and used to estimate the rms surface roughness. The data measurements are then compared with X-ray scatter measurements of the same samples. A summary of the data generated is presented, along with graphs showing changes in scatter on samples before and after cleaning. Results show that very smooth surfaces can be polished on the common substrate materials (from 2 to 10 Angstroms), and nickel appears to give the lowest visible light scatter.
NASA Astrophysics Data System (ADS)
He, Xiao Dong
This thesis studies light scattering processes off rough surfaces. Analytic models for reflection, transmission and subsurface scattering of light are developed. The results are applicable to realistic image generation in computer graphics. The investigation focuses on the basic issue of how light is scattered locally by general surfaces which are neither diffuse nor specular; Physical optics is employed to account for diffraction and interference which play a crucial role in the scattering of light for most surfaces. The thesis presents: (1) A new reflectance model; (2) A new transmittance model; (3) A new subsurface scattering model. All of these models are physically-based, depend on only physical parameters, apply to a wide range of materials and surface finishes and more importantly, provide a smooth transition from diffuse-like to specular reflection as the wavelength and incidence angle are increased or the surface roughness is decreased. The reflectance and transmittance models are based on the Kirchhoff Theory and the subsurface scattering model is based on Energy Transport Theory. They are valid only for surfaces with shallow slopes. The thesis shows that predicted reflectance distributions given by the reflectance model compare favorably with experiment. The thesis also investigates and implements fast ways of computing the reflectance and transmittance models. Furthermore, the thesis demonstrates that a high level of realistic image generation can be achieved due to the physically -correct treatment of the scattering processes by the reflectance model.
Structure-property evolution during polymer crystallization
NASA Astrophysics Data System (ADS)
Arora, Deepak
The main theme of this research is to understand the structure-property evolution during crystallization of a semicrystalline thermoplastic polymer. A combination of techniques including rheology, small angle light scattering, differential scanning calorimetry and optical microscopy are applied to follow the mechanical and optical properties along with crystallinity and the morphology. Isothermal crystallization experiments on isotactic poly-1-butene at early stages of spherulite growth provide quantitative information about nucleation density, volume fraction of spherulites and their crystallinity, and the mechanism of connecting into a sample spanning structure. Optical microscopy near the fluid-to-solid transition suggests that the transition, as determined by time-resolved mechanical spectroscopy, is not caused by packing/jamming of spherulites but by the formation of a percolating network structure. The effect of strain, Weissenberg number (We ) and specific mechanical work (w) on rate of crystallization (nucleation followed by growth) and on growth of anisotropy was studied for shear-induced crystallization of isotactic poly-1-butene. The samples were sheared for a finite strain at the beginning of the experiment and then crystallized without further flow (Janeschitz-Kriegl protocol). Strain requirements to attain steady state/leveling off of the rate of crystallization were found to be much larger than the strain needed to achieve steady state of flow. The large strain and We>1 criteria were also observed for morphological transition from spherulitic growth to oriented growth. An apparatus for small angle light scattering (SALS) and light transmission measurements under shear was built and tested at the University of Massachusetts Amherst. As a new development, the polarization direction can be rotated by a liquid crystal polarization rotator (LCPR) with a short response time of 20 ms. The experiments were controlled and analyzed with a LabVIEW(TM) based code (LabVIEW(TM) 7.1) in real time. The SALS apparatus was custom built for ExxonMobil Research in Clinton NJ.
Light scattering properties of spheroidal particles
NASA Technical Reports Server (NTRS)
Asano, S.
1979-01-01
In the present paper, the light scattering characteristics of spheroidal particles are evaluated within the framework of a scattering theory developed for a homogeneous isotropic spheroid. This approach is shown to be well suited for computing the scattering quantities of spheroidal particles of fairly large sizes (up to a size parameter of 30). The effects of particle size, shape, index of refraction, and orientation on the scattering efficiency factors and the scattering intensity functions are studied and interpreted physically. It is shown that, in the case of oblique incidence, the scattering properties of a long slender prolate spheroid resemble those of an infinitely long circular cylinder.
NASA Astrophysics Data System (ADS)
Abdallah, Omar; Qananwah, Qasem; Abo Alam, Kawther; Bolz, Armin
2010-04-01
This paper describes the development of an early detection method for probing pathological tissue variations. The method could be used for classifying various tissue alteration namely tumors tissue or skin disorders. The used approach is based on light scattering and absorption spectroscopy. Spectral content of the scattered light provides diagnostic information about the tissue contents. The importance of this method is using a safe light that has less power than the used in the imaging methods that will enable the frequent examination of tissue, while the exiting modalities have drawbacks like ionization, high cost, time-consuming, and agents' usage. A modality for mapping the oxygen saturation distribution in tissues noninvasively is new in this area of research, since this study focuses on the oxygen molecule in the tissue which supposed to be homogenously distributed through the tissues. Cancers may cause greater vascularization and greater oxygen consumption than in normal tissue. Therefore, oxygen existence and homogeneity will be alternated depending on the tissue state. In the proposed system, the signal was extracted after illuminating the tissue by light emitting diodes (LED's) that emits light in two wavelengths, red (660 nm) and infrared (880 nm). The absorption in these wavelengths is mainly due to oxyhemoglobin (HbO2) and deoxyhemoglobin (Hb) while other blood and tissue contents nearly have low effect on the signal. The backscattered signal which is received by a photodiodes array (128 PDs) was measured and processed using LabVIEW. Photoplethysmogram (PPG) signals have been measured at different locations. These signals will be used to differentiate between the normal and the pathological tissues. Variations in hemoglobin concentration and blood perfusion will also be used as an important indication feature for this purpose.
Xiangjie, Zhao; Cangli, Liu; Jiazhu, Duan; Jiancheng, Zeng; Dayong, Zhang; Yongquan, Luo
2014-06-16
Polymer network liquid crystal (PNLC) was one of the most potential liquid crystal for submillisecond response phase modulation, which was possible to be applied in submillisecond response phase only spatial light modulator. But until now the light scattering when liquid crystal director was reoriented by external electric field limited its phase modulation application. Dynamic response of phase change when high voltage was applied was also not elucidated. The mechanism that determines the light scattering was studied by analyzing the polymer network morphology by SEM method. Samples were prepared by varying the polymerization temperature, UV curing intensity and polymerization time. The morphology effect on the dynamic response of phase change was studied, in which high voltage was usually applied and electro-striction effect was often induced. The experimental results indicate that the polymer network morphology was mainly characterized by cross linked single fibrils, cross linked fibril bundles or even both. Although the formation of fibril bundle usually induced large light scattering, such a polymer network could endure higher voltage. In contrast, although the formation of cross linked single fibrils induced small light scattering, such a polymer network cannot endure higher voltage. There is a tradeoff between the light scattering and high voltage endurance. The electro-optical properties such as threshold voltage and response time were taken to verify our conclusion. For future application, the monomer molecular structure, the liquid crystal solvent and the polymerization conditions should be optimized to generate optimal polymer network morphology.
Light scattering in optical CT scanning of Presage dosimeters
NASA Astrophysics Data System (ADS)
Xu, Y.; Adamovics, J.; Cheeseborough, J. C.; Chao, K. S.; Wuu, C. S.
2010-11-01
The intensity of the scattered light from the Presage dosimeters was measured using a Thorlabs PM100D optical power meter (Thorlabs Inc, Newton, NJ) with an optical sensor of 1 mm diameter sensitive area. Five Presage dosimeters were made as cylinders of 15.2 cm, 10 cm, 4 cm diameters and irradiated with 6 MV photons using a Varian Clinac 2100EX. Each dosimeter was put into the scanning tank of an OCTOPUS" optical CT scanner (MGS Research Inc, Madison, CT) filled with a refractive index matching liquid. A laser diode was positioned at one side of the water tank to generate a stationary laser beam of 0.8 mm width. On the other side of the tank, an in-house manufactured positioning system was used to move the optical sensor in the direction perpendicular to the outgoing laser beam from the dosimeters at an increment of 1 mm. The amount of scattered photons was found to be more than 1% of the primary light signal within 2 mm from the laser beam but decreases sharply with increasing off-axis distance. The intensity of the scattered light increases with increasing light attenuations and/or absorptions in the dosimeters. The scattered light at the same off-axis distance was weaker for dosimeters of larger diameters and for larger detector-to-dosimeter distances. Methods for minimizing the effect of the light scattering in different types of optical CT scanners are discussed.
Absorbance and light scattering of lenses organ cultured with glucose.
Alghamdi, Ali Hendi Sahmi; Mohamed, Hasabelrasoul; Sledge, Samiyyah M; Borchman, Douglas
2018-06-06
Purpose/Aim: Diabetes is one of the major factors related to cataract. Our aim was to determine if the attenuation of light through glucose treated lenses was due to light scattering from structural changes or absorbance from metabolic changes. Human and rat lenses were cultured in a medium with and without 55 mM glucose for a period of five days. Absorbance and light scattering were measured using a ultraviolet spectrometer. Aldose reductase and catalase activity, RAGE, and glutathione were measured using classical assays. Almost all of the glucose related attenuation of light through the human lens was due to light scattering from structural changes. Glucose treatment caused three absorbance band to appear at 484, 540 to 644 and 657 nm in both the rat and human lens. The optimum time point for equilibration of human lenses was found to be between 2 and 3 days in organ culture. Glucose caused a more significant effect on the opacity of human lenses compared with rat lenses. Since the levels of glutathione, catalase and aldose reductase were reduced in glucose treated rat lenses compared with untreated lenses, glucose may have caused oxidative stress on the rat lens. The absorbance and light scattering of glucose treated lenses in organ culture were quantitated for the first time which could be important for future studies designed to test the efficacy of agents to ameliorate the opacity. Almost all of the glucose related attenuation of light through the human lens was due to light scattering from structural changes and not absorbance from metabolic changes. Glucose caused a more significant effect on the opacity of human lenses compared with rat lenses. The lens model employed could be used to study the efficacy of agents that potentially ameliorate lens opacity.
Battistoni, Andrea; Bencivenga, Filippo; Fioretto, Daniele; Masciovecchio, Claudio
2014-10-15
In this Letter, we present a simple method to avoid the well-known spurious contributions in the Brillouin light scattering (BLS) spectrum arising from the finite aperture of collection optics. The method relies on the use of special spatial filters able to select the scattered light with arbitrary precision around a given value of the momentum transfer (Q). We demonstrate the effectiveness of such filters by analyzing the BLS spectra of a reference sample as a function of scattering angle. This practical and inexpensive method could be an extremely useful tool to fully exploit the potentiality of Brillouin acoustic spectroscopy, as it will easily allow for effective Q-variable experiments with unparalleled luminosity and resolution.
Light scattering study of rheumatoid arthritis
DOE Office of Scientific and Technical Information (OSTI.GOV)
Beuthan, J; Netz, U; Minet, O
The distribution of light scattered by finger joints is studied in the near-IR region. It is shown that variations in the optical parameters of the tissue (scattering coefficient {mu}{sub s}, absorption coefficient {mu}{sub a}, and anisotropy factor g) depend on the presence of the rheumatoid arthritis (RA). At the first stage, the distribution of scattered light was measured in diaphanoscopic experiments. The convolution of a Gaussian error function with the scattering phase function proved to be a good approximation of the data obtained. Then, a new method was developed for the reconstruction of distribution of optical parameters in the fingermore » cross section. Model tests of the quality of this reconstruction method show good results. (laser biology and medicine)« less
Monte Carlo simulation of light reflection from cosmetic powders on the skin
NASA Astrophysics Data System (ADS)
Okamoto, Takashi; Motoda, Masafumi; Igarashi, Takanori; Nakao, Keisuke
2011-07-01
The reflection and scattering properties of light incident on skin covered with powder particles have been investigated. A three-layer skin structure with a spot is modeled, and the propagation of light in the skin and the scattering of light by particles on the skin surface are simulated by means of a Monte Carlo method. Under the condition in which only single scattering of light occurs in the powder layer, the reflection spectra of light from the skin change dramatically with the size of powder particles. The color difference between normal skin and spots is found to diminish more when powder particles smaller than the wavelength of light are used. It is shown that particle polydispersity suppresses substantially the extreme spectral change caused by monodisperse particles with a size comparable to the light wavelength.
Research Update: A minimal region of squid reflectin for vapor-induced light scattering
NASA Astrophysics Data System (ADS)
Dennis, Patrick B.; Singh, Kristi M.; Vasudev, Milana C.; Naik, Rajesh R.; Crookes-Goodson, Wendy J.
2017-12-01
Reflectins are a family of proteins found in the light manipulating cells of cephalopods. These proteins are made up of a series of conserved repeats that contain highly represented amino acids thought to be important for function. Previous studies demonstrated that recombinant reflectins cast into thin films produced structural colors that could be dynamically modulated via changing environmental conditions. In this study, we demonstrate light scattering from reflectin films following exposure to a series of water vapor pulses. Analysis of film surface topography shows that the induction of light scatter is accompanied by self-assembly of reflectins into micro- and nanoscale features. Using a reductionist strategy, we determine which reflectin repeats and sub-repeats are necessary for these events following water vapor pulsing. With this approach, we identify a singly represented, 23-amino acid region in reflectins as being sufficient to recapitulate the light scattering properties observed in thin films of the full-length protein. Finally, the aqueous stability of reflectin films is leveraged to show that pre-exposure to buffers of varying pH can modulate the ability of water vapor pulses to induce light scatter and protein self-assembly.
Siderius, Daniel W; Krekelberg, William P; Roberts, Christopher J; Shen, Vincent K
2012-05-07
Protein-protein interactions in solution may be quantified by the osmotic second virial coefficient (OSVC), which can be measured by various experimental techniques including light scattering. Analysis of Rayleigh light scattering measurements from such experiments requires identification of a scattering volume and the thermodynamic constraints imposed on that volume, i.e., the statistical mechanical ensemble in which light scattering occurs. Depending on the set of constraints imposed on the scattering volume, one can obtain either an apparent OSVC, A(2,app), or the true thermodynamic OSVC, B(22)(osm), that is rigorously defined in solution theory [M. A. Blanco, E. Sahin, Y. Li, and C. J. Roberts, J. Chem. Phys. 134, 225103 (2011)]. However, it is unclear to what extent A(2,app) and B(22)(osm) differ, which may have implications on the physical interpretation of OSVC measurements from light scattering experiments. In this paper, we use the multicomponent hard-sphere model and a well-known equation of state to directly compare A(2,app) and B(22)(osm). Our results from the hard-sphere equation of state indicate that A(2,app) underestimates B(22)(osm), but in a systematic manner that may be explained using fundamental thermodynamic expressions for the two OSVCs. The difference between A(2,app) and B(22)(osm) may be quantitatively significant, but may also be obscured in experimental application by statistical uncertainty or non-steric interactions. Consequently, the two OSVCs that arise in the analysis of light scattering measurements do formally differ, but in a manner that may not be detectable in actual application.
NASA Astrophysics Data System (ADS)
Sahoo, Sujit Kumar; Tang, Dongliang; Dang, Cuong
2018-02-01
Large field of view multispectral imaging through scattering medium is a fundamental quest in optics community. It has gained special attention from researchers in recent years for its wide range of potential applications. However, the main bottlenecks of the current imaging systems are the requirements on specific illumination, poor image quality and limited field of view. In this work, we demonstrated a single-shot high-resolution colour-imaging through scattering media using a monochromatic camera. This novel imaging technique is enabled by the spatial, spectral decorrelation property and the optical memory effect of the scattering media. Moreover the use of deconvolution image processing further annihilate above-mentioned drawbacks arise due iterative refocusing, scanning or phase retrieval procedures.
NASA Astrophysics Data System (ADS)
Wang, Jiao; Xue, Jinhua; Xiao, Xilin; Xu, Li; Jiang, Min; Peng, Pengcheng; Liao, Lifu
2017-12-01
The coordination reaction of thorium (IV) with a ditopic bidentate ligand to form supramolecular polymer was studied by resonance light scattering (RLS) spectra, second-order scattering (SOS) spectra and frequency-doubling scattering (FDS) spectra, respectively. The ditopic bidentate ligand is isophthalaldehyde-tetrapyrrole (IPTP). It was synthesized through a condensation reaction of isophthalaldehyde with pyrrole. The formation of supramolecular polymer results in remarkable intensity enhancements of the three light scattering signals. The maximum scattering wavelengths of RLS, FDS and SOS were 290, 568 and 340 nm, respectively. The reaction was used to establish new light scattering methods for the determination of thorium (IV) by using IPTP as probe. Under optimum conditions, the intensity enhancements of RLS, SOS and FDS were directly proportional to the concentration of thorium (IV) in the ranges of 0.01 to 1.2 μg mL- 1, 0.05 to 1.2 μg mL- 1 and 0.05 to 1.2 μg mL- 1, respectively. The detection limits were 0.003 μg mL- 1, 0.012 μg mL- 1 and 0.021 μg mL- 1, respectively. The methods were suitable for analyzing thorium (IV) in actual samples. The results show acceptable recoveries and precision compared with a reference method.
3. View Looking Northwest Showing Base of Derrick and Scattered ...
3. View Looking Northwest Showing Base of Derrick and Scattered Pipe Fragments in Foreground - David Renfrew Oil Rig, East side of Connoquenessing Creek, 0.4 mile North of confluence with Thorn Creek, Renfrew, Butler County, PA
Uribe-Patarroyo, Néstor; Bouma, Brett E.
2015-01-01
We present a new technique for the correction of nonuniform rotation distortion in catheter-based optical coherence tomography (OCT), based on the statistics of speckle between A-lines using intensity-based dynamic light scattering. This technique does not rely on tissue features and can be performed on single frames of data, thereby enabling real-time image correction. We demonstrate its suitability in a gastrointestinal balloon-catheter OCT system, determining the actual rotational speed with high temporal resolution, and present corrected cross-sectional and en face views showing significant enhancement of image quality. PMID:26625040
2003-05-10
These images, from David Weitz’s liquid crystal research, show ordered uniform sized droplets (upper left) before they are dried from their solution. After the droplets are dried (upper right), they are viewed with crossed polarizers that show the deformation caused by drying, a process that orients the bipolar structure of the liquid crystal within the droplets. When an electric field is applied to the dried droplets (lower left), and then increased (lower right), the liquid crystal within the droplets switches its alignment, thereby reducing the amount of light that can be scattered by the droplets when a beam is shone through them.
Dependence of Surface Contrast on Emission Angle in Cassini ISS 938-nm Images of Titan
NASA Technical Reports Server (NTRS)
Fussner, S.; McEwen, A.; Perry, J.; Turtle, E.; Dawson, D.; Porco, C.; West, R.
2005-01-01
Titan, the largest of Saturn s moons, is one of the most difficult solid surfaces in the Solar System to study. It is shrouded in a thick atmosphere with fine haze particles extending up to 500 km. [1] The atmosphere itself is rich in methane, which allows clear viewing of the surface only through narrow "windows" in the methane spectrum. Even in these methane windows, the haze absorbs and scatters light, blurring surface features and reducing the contrast of images. The haze optical depth is high at visible wavelengths, and decreases at longer (infrared) wavelengths. [2
Elastic light single-scattering spectroscopy for detection of dysplastic tissues
NASA Astrophysics Data System (ADS)
Canpolat, Murat; Denkçeken, Tuba; Akman, Ayşe.; Alpsoy, Erkan; Tuncer, Recai; Akyüz, Mahmut; Baykara, Mehmet; Yücel, Selçuk; Başsorgun, Ibrahim; ćiftçioǧlu, M. Akif; Gökhan, Güzide Ayşe.; Gürer, ElifInanç; Peştereli, Elif; Karaveli, Šeyda
2013-11-01
Elastic light single-scattering spectroscopy (ELSSS) system has been developed and tested in diagnosis of cancerous tissues of different organs. ELSSS system consists of a miniature visible light spectrometer, a single fiber optical probe, a halogen tungsten light source and a laptop. Measurements were performed on excised brain, skin, cervix and prostate tumor specimens and surrounding normal tissues. Single fiber optical probe with a core diameter of 100 μm was used to deliver white light to and from tissue. Single optical fiber probe mostly detects singly scattered light from tissue rather than diffused light. Therefore, measured spectra are sensitive to size of scatters in tissue such as cells, nuclei, mitochondria and other organelles of cells. Usually, nuclei of tumor cells are larger than nuclei of normal cells. Therefore, spectrum of singly scattered light of tumor tissue is different than normal tissue. The spectral slopes were shown to be positive for normal brain, skin and prostate and cervix tissues and negative for the tumors of the same tissues. Signs of the spectral slopes were used as a discrimination parameter to differentiate tumor from normal tissues for the three organ tissues. Sensitivity and specificity of the system in differentiation between tumors from normal tissues were 93% and %100 for brain, 87% and 85% for skin, 93.7% and 46.1% for cervix and 98% and 100% for prostate.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Shi, L; Zhu, L; Vedantham, S
2016-06-15
Purpose: The image quality of dedicated cone-beam breast CT (CBBCT) is fundamentally limited by substantial x-ray scatter contamination, resulting in cupping artifacts and contrast-loss in reconstructed images. Such effects obscure the visibility of soft-tissue lesions and calcifications, which hinders breast cancer detection and diagnosis. In this work, we propose to suppress x-ray scatter in CBBCT images using a deterministic forward projection model. Method: We first use the 1st-pass FDK-reconstructed CBBCT images to segment fibroglandular and adipose tissue. Attenuation coefficients are assigned to the two tissues based on the x-ray spectrum used for imaging acquisition, and is forward projected to simulatemore » scatter-free primary projections. We estimate the scatter by subtracting the simulated primary projection from the measured projection, and then the resultant scatter map is further refined by a Fourier-domain fitting algorithm after discarding untrusted scatter information. The final scatter estimate is subtracted from the measured projection for effective scatter correction. In our implementation, the proposed scatter correction takes 0.5 seconds for each projection. The method was evaluated using the overall image spatial non-uniformity (SNU) metric and the contrast-to-noise ratio (CNR) with 5 clinical datasets of BI-RADS 4/5 subjects. Results: For the 5 clinical datasets, our method reduced the SNU from 7.79% to 1.68% in coronal view and from 6.71% to 3.20% in sagittal view. The average CNR is improved by a factor of 1.38 in coronal view and 1.26 in sagittal view. Conclusion: The proposed scatter correction approach requires no additional scans or prior images and uses a deterministic model for efficient calculation. Evaluation with clinical datasets demonstrates the feasibility and stability of the method. These features are attractive for clinical CBBCT and make our method distinct from other approaches. Supported partly by NIH R21EB019597, R21CA134128 and R01CA195512.The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.« less
Introduction to light and optical theories
USDA-ARS?s Scientific Manuscript database
Light scattering occurs as a result of the interaction of photons with matter, and it is dependent on the structure and chemical composition of the material. Over the past 15 years, significant progress and numerous applications in light scattering have been made for assessing properties, quality an...
Enhanced coupling of light into a turbid medium through microscopic interface engineering
Thompson, Jonathan V.; Hokr, Brett H.; Kim, Wihan; Ballmann, Charles W.; Applegate, Brian E.; Jo, Javier; Yamilov, Alexey; Cao, Hui; Scully, Marlan O.; Yakovlev, Vladislav V.
2017-01-01
There are many optical detection and sensing methods used today that provide powerful ways to diagnose, characterize, and study materials. For example, the measurement of spontaneous Raman scattering allows for remote detection and identification of chemicals. Many other optical techniques provide unique solutions to learn about biological, chemical, and even structural systems. However, when these systems exist in a highly scattering or turbid medium, the optical scattering effects reduce the effectiveness of these methods. In this article, we demonstrate a method to engineer the geometry of the optical interface of a turbid medium, thereby drastically enhancing the coupling efficiency of light into the material. This enhanced optical coupling means that light incident on the material will penetrate deeper into (and through) the medium. It also means that light thus injected into the material will have an enhanced interaction time with particles contained within the material. These results show that, by using the multiple scattering of light in a turbid medium, enhanced light–matter interaction can be achieved; this has a direct impact on spectroscopic methods such as Raman scattering and fluorescence detection in highly scattering regimes. Furthermore, the enhanced penetration depth achieved by this method will directly impact optical techniques that have previously been limited by the inability to deposit sufficient amounts of optical energy below or through highly scattering layers. PMID:28701381
NASA Astrophysics Data System (ADS)
Yokokawa, Takumi; Nishidate, Izumi
2016-04-01
We investigate a method to evaluate light-scattering properties and chromophore concentrations in human skin tissue through diffuse reflectance spectroscopy using the reflectance signals acquired at isosbestic wavelengths of hemoglobin (420, 450, 500, and 585 nm). In the proposed method, Monte Carlo simulation-based empirical formulas are used to specify the scattering parameters of skin tissue, such as the scattering amplitude a and the scattering power b, as well as the concentration of melanin C m and the total blood concentration C tb. The use of isosbestic wavelengths of hemoglobin enables the values of C m, C tb, a, and b to be estimated independently of the oxygenation of hemoglobin. The spectrum of the reduced scattering coefficient is reconstructed from the scattering parameters. Experiments using in vivo human skin tissues were performed to confirm the feasibility of the proposed method for evaluating the changes in scattering properties and chromophore concentrations in skin tissue. The experimental results revealed that light scattering is significantly reduced by the application of a glycerol solution, which indicates an optical clearing effect due to osmotic dehydration and the matching of the refractive indices of scatterers in the epidermis.
Agrawal, Anant; Majdi, Joseph; Clouse, Kathleen A; Stantchev, Tzanko
2018-05-23
Optical biosensors based on scattered-light measurements are being developed for rapid and label-free detection of single virions captured from body fluids. Highly controlled, stable, and non-biohazardous reference materials producing virus-like signals are valuable tools to calibrate, evaluate, and refine the performance of these new optical biosensing methods. To date, spherical polymer nanoparticles have been the only non-biological reference materials employed with scattered-light biosensing techniques. However, pathogens like filoviruses, including the Ebola virus, are far from spherical and their shape strongly affects scattered-light signals. Using electron beam lithography, we fabricated nanostructures resembling individual filamentous virions attached to a biosensing substrate (silicon wafer overlaid with silicon oxide film) and characterized their dimensions with scanning electron and atomic force microscopes. To assess the relevance of these nanostructures, we compared their signals across the visible spectrum to signals recorded from Ebola virus-like particles which exhibit characteristic filamentous morphology. We demonstrate the highly stable nature of our nanostructures and use them to obtain new insights into the relationship between virion dimensions and scattered-light signal.
First scattered-light image of the debris disk around HD 131835 with the Gemini Planet Imager
Hung, Li -Wei; Duchêne, Gaspard; Arriaga, Pauline; ...
2015-12-09
Here, we present the first scattered-light image of the debris disk around HD 131835 in the H band using the Gemini Planet Imager. HD 131835 is a ~15 Myr old A2IV star at a distance of ~120 pc in the Sco-Cen OB association. We detect the disk only in polarized light and place an upper limit on the peak total intensity. No point sources resembling exoplanets were identified. Compared to its mid-infrared thermal emission, in scattered light the disk shows similar orientation but different morphology. The scattered-light disk extends from ~75 to ~210 AU in the disk plane with roughlymore » flat surface density. Our Monte Carlo radiative transfer model can describe the observations with a model disk composed of a mixture of silicates and amorphous carbon. In addition to the obvious brightness asymmetry due to stronger forward scattering, we discover a weak brightness asymmetry along the major axis, with the northeast side being 1.3 times brighter than the southwest side at a 3σ level.« less
FIRST SCATTERED-LIGHT IMAGE OF THE DEBRIS DISK AROUND HD 131835 WITH THE GEMINI PLANET IMAGER
DOE Office of Scientific and Technical Information (OSTI.GOV)
Hung, Li-Wei; Arriaga, Pauline; Fitzgerald, Michael P.
2015-12-10
We present the first scattered-light image of the debris disk around HD 131835 in the H band using the Gemini Planet Imager. HD 131835 is a ∼15 Myr old A2IV star at a distance of ∼120 pc in the Sco-Cen OB association. We detect the disk only in polarized light and place an upper limit on the peak total intensity. No point sources resembling exoplanets were identified. Compared to its mid-infrared thermal emission, in scattered light the disk shows similar orientation but different morphology. The scattered-light disk extends from ∼75 to ∼210 AU in the disk plane with roughly flatmore » surface density. Our Monte Carlo radiative transfer model can describe the observations with a model disk composed of a mixture of silicates and amorphous carbon. In addition to the obvious brightness asymmetry due to stronger forward scattering, we discover a weak brightness asymmetry along the major axis, with the northeast side being 1.3 times brighter than the southwest side at a 3σ level.« less
NASA Astrophysics Data System (ADS)
Shang, Li; Dong, Shaojun
2008-03-01
A simple light scattering detection method for neurotransmitters has been developed, based on the growth of gold nanoparticles. Neurotransmitters (dopamine, L-dopa, noradrenaline and adrenaline) can effectively function as active reducing agents for generating gold nanoparticles, which result in enhanced light scattering signals. The strong light scattering of gold nanoparticles then allows the quantitative detection of the neurotransmitters simply by using a common spectrofluorometer. In particular, Au-nanoparticle seeds were added to facilitate the growth of nanoparticles, which was found to enhance the sensing performance greatly. Using this light scattering technique based on the seed-mediated growth of gold nanoparticles, detection limits of 4.4 × 10-7 M, 3.5 × 10-7 M, 4.1 × 10-7 M, and 7.7 × 10-7 M were achieved for dopamine, L-dopa, noradrenaline and adrenaline, respectively. The present strategy can be extended to detect other biologically important molecules in a very fast, simple and sensitive way, and may have potential applications in a wide range of fields.
On the interpolation of light-scattering responses from irregularly shaped particles
NASA Astrophysics Data System (ADS)
Videen, Gorden; Zubko, Evgenij; Arnold, Jessica A.; MacCall, Benjamin; Weinberger, Alycia J.; Shkuratov, Yuriy; Muñoz, Olga
2018-05-01
Common particle characteristics needed for many applications may include size, eccentricity, porosity and refractive index. Determining such characteristics from scattered light is a primary goal of remote sensing. For other applications, like differentiating a hazardous particle from the natural background, information about higher fidelity particle characteristics may be required, including specific shape or chemical composition. While a complete characterization of a particle system from its scattered light through the inversion process remains unachievable, great strides have been made in providing information in the form of constraints on particle characteristics. Recent advances have been made in quantifying the characteristics of polydispersions of irregularly shaped particles by making comparisons of the light-scattering signals from model simulant particles. We show that when the refractive index is changed, the light-scattering characteristics from polydispersions of such particles behave monotonically over relatively large parameter ranges compared with those of monodisperse distributions of particles having regular shapes, like spheres, spheroids, etc. This allows for their properties to be interpolated, which results in a significant reduction of the computational load when performing inversions.
Evaluation of illumination system uniformity for wide-field biomedical hyperspectral imaging
NASA Astrophysics Data System (ADS)
Sawyer, Travis W.; Siri Luthman, A.; E Bohndiek, Sarah
2017-04-01
Hyperspectral imaging (HSI) systems collect both spatial (morphological) and spectral (chemical) information from a sample. HSI can provide sensitive analysis for biological and medical applications, for example, simultaneously measuring reflectance and fluorescence properties of a tissue, which together with structural information could improve early cancer detection and tumour characterisation. Illumination uniformity is a critical pre-condition for quantitative data extraction from an HSI system. Non-uniformity can cause glare, specular reflection and unwanted shading, which negatively impact statistical analysis procedures used to extract abundance of different chemical species. Here, we model and evaluate several illumination systems frequently used in wide-field biomedical imaging to test their potential for HSI. We use the software LightTools and FRED. The analysed systems include: a fibre ring light; a light emitting diode (LED) ring; and a diffuse scattering dome. Each system is characterised for spectral, spatial, and angular uniformity, as well as transfer efficiency. Furthermore, an approach to measure uniformity using the Kullback-Leibler divergence (KLD) is introduced. The KLD is generalisable to arbitrary illumination shapes, making it an attractive approach for characterising illumination distributions. Although the systems are quite comparable in their spatial and spectral uniformity, the most uniform angular distribution is achieved using a diffuse scattering dome, yielding a contrast of 0.503 and average deviation of 0.303 over a ±60° field of view with a 3.9% model error in the angular domain. Our results suggest that conventional illumination sources can be applied in HSI, but in the case of low light levels, bespoke illumination sources may offer improved performance.
Effects of Different Viewing Conditions on Radiographic Interpretation
Moshfeghi, Mahkameh; Shahbazian, Majid; Sajadi, Soodabeh Sadat; Sajadi, Sepideh; Ansari, Hossein
2015-01-01
Objectives: Optimum viewing conditions facilitate identification of radiographic details and decrease the need for retakes, patients’ costs and radiation dose. This study sought to evaluate the effects of different viewing conditions on radiographic interpretation. Materials and Methods: This diagnostic study was performed by evaluating radiograph of a 7mm-thick aluminum block, in which 10 holes with 2mm diameters were randomly drilled with depths ranging from 0.05 mm to 0.50mm. The radiograph was viewed by four oral radiologists independently under four viewing conditions, including a white light viewing light box in a lit room, yellow light viewing light box in a lit room, white light viewing light box in a dark room and yellow light viewing light box in a dark room. Number of circular shadows observed on the film was recorded. The data were analyzed by two-way ANOVA. Results: The mean number of detected circular shadows was 6.75, 7.5, 7.25 and 7.75 in white light viewing light box in a lit room, white light viewing light box in a dark room, yellow light viewing light box in a lit room and yellow light viewing light box in a dark room, respectively. Although the surrounding illumination had statistically significant effect on the radiographic details (P≤0.03), the light color of the viewing light box had no significant effect on visibility of the radiographic details. Conclusion: White and yellow light of the viewing light box had no significant effect on visibility of the radiographic details but more information was obtained in a dark room. PMID:27507997
Effects of Different Viewing Conditions on Radiographic Interpretation.
Moshfeghi, Mahkameh; Shahbazian, Majid; Sajadi, Soodabeh Sadat; Sajadi, Sepideh; Ansari, Hossein
2015-11-01
Optimum viewing conditions facilitate identification of radiographic details and decrease the need for retakes, patients' costs and radiation dose. This study sought to evaluate the effects of different viewing conditions on radiographic interpretation. This diagnostic study was performed by evaluating radiograph of a 7mm-thick aluminum block, in which 10 holes with 2mm diameters were randomly drilled with depths ranging from 0.05 mm to 0.50mm. The radiograph was viewed by four oral radiologists independently under four viewing conditions, including a white light viewing light box in a lit room, yellow light viewing light box in a lit room, white light viewing light box in a dark room and yellow light viewing light box in a dark room. Number of circular shadows observed on the film was recorded. The data were analyzed by two-way ANOVA. The mean number of detected circular shadows was 6.75, 7.5, 7.25 and 7.75 in white light viewing light box in a lit room, white light viewing light box in a dark room, yellow light viewing light box in a lit room and yellow light viewing light box in a dark room, respectively. Although the surrounding illumination had statistically significant effect on the radiographic details (P≤0.03), the light color of the viewing light box had no significant effect on visibility of the radiographic details. White and yellow light of the viewing light box had no significant effect on visibility of the radiographic details but more information was obtained in a dark room.
Gravitational Wakes Sizes from Multiple Cassini Radio Occultations of Saturn's Rings
NASA Astrophysics Data System (ADS)
Marouf, E. A.; Wong, K. K.; French, R. G.; Rappaport, N. J.; McGhee, C. A.; Anabtawi, A.
2016-12-01
Voyager and Cassini radio occultation extinction and forward scattering observations of Saturn's C-Ring and Cassini Division imply power law particle size distributions extending from few millimeters to several meters with power law index in the 2.8 to 3.2 range, depending on the specific ring feature. We extend size determination to the elongated and canted particle clusters (gravitational wakes) known to permeate Saturn's A- and B-Rings. We use multiple Cassini radio occultation observations over a range of ring opening angle B and wake viewing angle α to constrain the mean wake width W and thickness/height H, and average ring area coverage fraction. The rings are modeled as randomly blocked diffraction screen in the plane normal to the incidence direction. Collective particle shadows define the blocked area. The screen's transmittance is binary: blocked or unblocked. Wakes are modeled as thin layer of elliptical cylinders populated by random but uniformly distributed spherical particles. The cylinders can be immersed in a "classical" layer of spatially uniformly distributed particles. Numerical simulations of model diffraction patterns reveal two distinct components: cylindrical and spherical. The first dominates at small scattering angles and originates from specific locations within the footprint of the spacecraft antenna on the rings. The second dominates at large scattering angles and originates from the full footprint. We interpret Cassini extinction and scattering observations in the light of the simulation results. We compute and remove contribution of the spherical component to observed scattered signal spectra assuming known particle size distribution. A large residual spectral component is interpreted as contribution of cylindrical (wake) diffraction. Its angular width determines a cylindrical shadow width that depends on the wake parameters (W,H) and the viewing geometry (α,B). Its strength constrains the mean fractional area covered (optical depth), hence constrains the mean wakes spacing. Self-consistent (W,H) are estimated using least-square fit to results from multiple occultations. Example results for observed scattering by several inner A-Ring features suggest particle clusters (wakes) that are few tens of meters wide and several meters thick.
Validating Lidar Depolorization Calibration using Solar Radiation Scattered by Ice Clouds
NASA Technical Reports Server (NTRS)
Liu, Zhao-Yang; McGill, Matthew; Hu, Yong-Xiang; Hostetter, Chris; Winker, David; Vaughan, Mark
2004-01-01
This letter proposes the use of solar background radiation scattered by ice clouds for validating space lidar depolarization calibration. The method takes advantage of the fact that the background light scattered by ice clouds is almost entirely unpolarized. The theory is examined with Cloud Physics Lidar (CPL) background light measurements.
Labview Interface Concepts Used in NASA Scientific Investigations and Virtual Instruments
NASA Technical Reports Server (NTRS)
Roth, Don J.; Parker, Bradford H.; Rapchun, David A.; Jones, Hollis H.; Cao, Wei
2001-01-01
This article provides an overview of several software control applications developed for NASA using LabVIEW. The applications covered here include (1) an Ultrasonic Measurement System for nondestructive evaluation of advanced structural materials, an Xray Spectral Mapping System for characterizing the quality and uniformity of developing photon detector materials, (2) a Life Testing System for these same materials, (3) and the instrument panel for an aircraft mounted Cloud Absorption Radiometer that measures the light scattered by clouds in multiple spectral bands. Many of the software interface concepts employed are explained. Panel layout and block diagram (code) strategies for each application are described. In particular, some of the more unique features of the applications' interfaces and source code are highlighted. This article assumes that the reader has a beginner-to-intermediate understanding of LabVIEW methods.
NASA Astrophysics Data System (ADS)
Ning, J. Q.; Zheng, C. C.; Zheng, L. X.; Xu, S. J.
2015-08-01
Spatially resolved Raman light scattering experiments were performed on a zinc-blende GaN/GaAs heterostructure with confocal micro-Raman scattering technique under the backscattering geometric configuration. By varying the illumination spot locations across the heterostructure interface, we found that the Raman light scattering spectral features change remarkably. The interface effect on the GaAs substrate manifested as a much broader lineshape of the transverse optical (TO) phonon mode. Two kinds of broadening mechanisms, namely, spatial correlation induced wave-vector relaxation effect and lattice-mismatch strain + compositional intermixing effect, have been identified. The former leads to the broadening of the TO mode at the low-energy side, whereas the latter accounts for the broadening at the high-energy side. The diffuse light scattering from the highly defective nucleation layer of GaN was found to produce a broad scattering background of the GaN TO mode. The methodology and conclusions of the present work could be applicable to Raman spectroscopic studies on other material interfaces.
Digital all-sky polarization imaging of partly cloudy skies.
Pust, Nathan J; Shaw, Joseph A
2008-12-01
Clouds reduce the degree of linear polarization (DOLP) of skylight relative to that of a clear sky. Even thin subvisual clouds in the "twilight zone" between clouds and aerosols produce a drop in skylight DOLP long before clouds become visible in the sky. In contrast, the angle of polarization (AOP) of light scattered by a cloud in a partly cloudy sky remains the same as in the clear sky for most cases. In unique instances, though, select clouds display AOP signatures that are oriented 90 degrees from the clear-sky AOP. For these clouds, scattered light oriented parallel to the scattering plane dominates the perpendicularly polarized Rayleigh-scattered light between the instrument and the cloud. For liquid clouds, this effect may assist cloud particle size identification because it occurs only over a relatively limited range of particle radii that will scatter parallel polarized light. Images are shown from a digital all-sky-polarization imager to illustrate these effects. Images are also shown that provide validation of previously published theories for weak (approximately 2%) polarization parallel to the scattering plane for a 22 degrees halo.
NASA Astrophysics Data System (ADS)
ten Brink, Harry; Henzing, Bas; Otjes, René; Weijers, Ernie
2018-01-01
The visibility on New Year's nights in the Netherlands is low during stagnant weather. This is due to the scattering and absorption of light by the aerosol-smoke from the fireworks. We made an assessment of the responsible aerosol-species. The investigation took place during the New Year's night of 2009. Measurements were made at a regional site in the centre of the country away from specific local sources. An Integrating Nephelometer measured the light-scattering by the inherent compounds after removal of water from the aerosol by drying the air. The actual light-scattering was determined in an open-air scatterometer; it was a factor of five higher than the ;dry; value. The difference in actual and ;dry; light-scattering can only be explained by water-uptake of the salty hygroscopic components of the aerosol. This hypothesis is substantiated by measurements of the composition of the aerosol. The size-dependent concentrations of the salty ionic species were determined on-line with a MARGA-;sizer;. These components were for a large part in particles in the size range that most effectively scatter light. The ;dry; light-scattering was exerted by the inorganic salt components and the sooty carbonaceous material alike. However, the salty products from the fireworks are hygroscopic and take up water at the high relative humidities occurring that night. This explains the fivefold larger light-scattering by the wet ambient aerosol as compared to that by the dry aerosol in the integrating nephelometer. The visibility, which is the inverse of the open-air scattering, is thus indirectly governed by the salty products of the fireworks due to their uptake of water. Under stagnant weather conditions during New Year's nights in the Netherlands both the aerosol concentrations and the relative humidity are high; this implies that the ionic species govern the low visibilities in general, be it via their uptake of water.
NASA Astrophysics Data System (ADS)
Lai, Puxiang; Suzuki, Yuta; Xu, Xiao; Wang, Lihong V.
2013-07-01
Scattering dominates light propagation in biological tissue, and therefore restricts both resolution and penetration depth in optical imaging within thick tissue. As photons travel into the diffusive regime, typically 1 mm beneath human skin, their trajectories transition from ballistic to diffusive due to the increased number of scattering events, which makes it impossible to focus, much less track, photon paths. Consequently, imaging methods that rely on controlled light illumination are ineffective in deep tissue. This problem has recently been addressed by a novel method capable of dynamically focusing light in thick scattering media via time reversal of ultrasonically encoded (TRUE) diffused light. Here, using photorefractive materials as phase conjugate mirrors, we show a direct visualization and dynamic control of optical focusing with this light delivery method, and demonstrate its application for focused fluorescence excitation and imaging in thick turbid media. These abilities are increasingly critical for understanding the dynamic interactions of light with biological matter and processes at different system levels, as well as their applications for biomedical diagnosis and therapy.
The Dynamics of Disorder-Order Transition in Hard Sphere Colloidal Dispersions
NASA Technical Reports Server (NTRS)
Chaikin, Paul M.; Zhu, Jixiang; Cheng, Zhengdong; Phan, See-Eng; Russel, William B.; Lant, Christian T.; Doherty, Michael P.; Meyer, William V.; Rogers, Richard; Cannell, D. S.;
1998-01-01
The Physics of Hard Spheres Experiment (PHaSE) seeks a complete understanding of the entropically driven disorder-order transition in hard sphere colloidal dispersions. The light scattering instrument designed for flight collects Bragg and low angle light scattering in the forward direction via a CCD camera and performs conventional static and dynamic light scattering at 10-160 deg. through fiber optic cables. Here we report on the kinetics of nucleation and growth extracted from time-resolved Bragg images and measurements of the elastic modulus of crystalline phases obtained by monitoring resonant responses to sinusoidal forcing through dynamic light scattering. Preliminary analysis of the former indicates a significant difference from measurements on the ground, while the latter confirms nicely laboratory experiments with the same instrument and predictions from computer simulations.
Direct Measurement of Scattered Light Effect on the Sensitivity in TAMA300
NASA Astrophysics Data System (ADS)
Takahashi, R.; Arai, Koji; Kawamaru, Seiji; Smith, Michael R.
2003-07-01
Laser interferometer gravitational wave detectors need vacuum tubes through which the laser beams pass. The light scattered from the arm cavity mirrors will make multiple reflections from the inside wall of the polished tube back onto the mirrors causing phase noise on the interferometer output beam. The TAMA300 has two 300-m length arms enclosed by vacuum tubes. By vibrating one of the tubes of the TAMA300, we directly observed the effect of scattered light on the displacement sensitivity. It was found that a tube vibration amplitude of 5.6 µm at 776.5 Hz increased the mirror displacement noise to 1.2 × 10-17 m. This noise level is consistent with the calculated noise due to the scattered light effect.
Laser light scattering instrument advanced technology development
NASA Technical Reports Server (NTRS)
Wallace, J. F.
1993-01-01
The objective of this advanced technology development (ATD) project has been to provide sturdy, miniaturized laser light scattering (LLS) instrumentation for use in microgravity experiments. To do this, we assessed user requirements, explored the capabilities of existing and prospective laser light scattering hardware, and both coordinated and participated in the hardware and software advances needed for a flight hardware instrument. We have successfully breadboarded and evaluated an engineering version of a single-angle glove-box instrument which uses solid state detectors and lasers, along with fiber optics, for beam delivery and detection. Additionally, we have provided the specifications and written verification procedures necessary for procuring a miniature multi-angle LLS instrument which will be used by the flight hardware project which resulted from this work and from this project's interaction with the laser light scattering community.
Surface roughness measurement in the submicrometer range using laser scattering
NASA Astrophysics Data System (ADS)
Wang, S. H.; Quan, Chenggen; Tay, C. J.; Shang, H. M.
2000-06-01
A technique for measuring surface roughness in the submicrometer range is developed. The principle of the method is based on laser scattering from a rough surface. A telecentric optical setup that uses a laser diode as a light source is used to record the light field scattered from the surface of a rough object. The light intensity distribution of the scattered band, which is correlated to the surface roughness, is recorded by a linear photodiode array and analyzed using a single-chip microcomputer. Several sets of test surfaces prepared by different machining processes are measured and a method for the evaluation of surface roughness is proposed.
NASA Technical Reports Server (NTRS)
1978-01-01
The practicability of using a classical light-scattering technique, involving comparison of angular scattering intensity patterns with theoretically determined Mie and Rayleight patterns, to detect discrete soot particles (diameter less than 50 nm) in premixed propane/air and propane/oxygen-helium flames is considered. The experimental apparatus employed in this investigation included a laser light source, a flat-flame burner, specially coated optics, a cooled photomultiplier detector, and a lock-in voltmeter readout. Although large, agglomerated soot particles were detected and sized, it was not possible to detect small, discrete particles. The limiting factor appears to be background scattering by the system's optics.
Methods for assessing forward and backward light scatter in patients with cataract.
Crnej, Alja; Hirnschall, Nino; Petsoglou, Con; Findl, Oliver
2017-08-01
To compare objective methods for assessing backward and forward light scatter and psychophysical tests in patients with cataracts. Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom. Prospective case series. This study included patients scheduled for cataract surgery. Lens opacities were grouped into predominantly nuclear sclerotic, cortical, posterior subcapsular, and mixed cataracts. Backward light scatter was assessed using a rotating Scheimpflug imaging technique (Pentacam HR), forward light scatter using a straylight meter (C-Quant), and straylight using the double-pass method (Optical Quality Analysis System, point-spread function [PSF] meter). The results were correlated with visual acuity under photopic conditions as well as photopic and mesopic contrast sensitivity. The study comprised 56 eyes of 56 patients. The mean age of the 23 men and 33 women was 71 years (range 48 to 84 years). Two patients were excluded. Of the remaining, 15 patients had predominantly nuclear sclerotic cataracts, 13 had cortical cataracts, 11 had posterior subcapsular cataracts, and 15 had mixed cataracts. Correlations between devices were low. The highest correlation was between PSF meter measurements and Scheimpflug measurements (r = 0.32). The best correlation between corrected distance visual acuity was with the PSF meter (r = 0.45). Forward and backward light-scatter measurements cannot be used interchangeably. Scatter as an aspect of quality of vision was independent of acuity. Measuring forward light scatter with the straylight meter can be a useful additional tool in preoperative decision-making. Copyright © 2017 ASCRS and ESCRS. Published by Elsevier Inc. All rights reserved.
Scattered Light Polarimetry of Exoplanets
NASA Astrophysics Data System (ADS)
Wiktorowicz, S.
2014-12-01
The last decade has witnessed an explosion in atmospheric characterization of spatially unresolved exoplanets using transmission spectra of transiting planets, but understanding has been hampered by degeneracies resolvable through blue optical observations. Here, scattered light is more important than the Wien tail of re-radiated thermal emission. Therefore, the next frontier in exoplanet characterization lies in the direct detection of scattered light. The polarization state of starlight scattered by a planetary atmosphere distinguishes it from the direct light from the host star, and the inherently differential nature of polarimetry reduces systematic effects to the point where ground-based detections are possible. Furthermore, polarimetry is uniquely sensitive to the size distribution, shape, and chemical composition of atmospheric cloud particles as well as to the scattering optical depth. I will review the current state of exoplanet polarimetry, which is dominated not by photon noise but by non-Gaussian systematic effects. Ground-based detection of order ten exoplanet photons relative to the host star's million requires dense orbital phase coverage and therefore long observing programs. However, variability inherent in the host star, interstellar medium, Earth's atmosphere, the telescope, and the instrument at all timescales must be measured and subtracted in order to definitively uncover scattered light from the exoplanet. While polarimetry is in principle sensitive to exoplanets regardless of orbital inclination, repeated observations of transiting exoplanet systems during secondary eclipse events are required to measure the polarimetric variability of the system that cannot be due to the planet. The emergence of scattered light polarimetry as a robust tool for the study of exoplanet atmospheres, and eventually surfaces, therefore requires diligent attention to the role of systematic effects.
Method for identifying and probing phase transitions in materials
Asay, Blaine W.; Henson, Bryan F.; Sander, Robert K.; Robinson, Jeanne M.; Son, Steven F.; Dickson, Peter M.
2002-01-01
The present invention includes a method for identifying and probing phase transitions in materials. A polymorphic material capable of existing in at least one non-centrosymmetric phase is interrogated with a beam of laser light at a chosen wavelength and frequency. A phase transition is induced in the material while it is interrogated. The intensity of light scattered by the material and having a wavelength equal to one half the wavelength of the interrogating laser light is detected. If the phase transition results in the production of a non-centrosymmetric phase, the intensity of this scattered light increases; if the phase transition results in the disappearance of a non-centrosymmetric phase, the intensity of this scattered light decreases.
An empirical correction for moderate multiple scattering in super-heterodyne light scattering.
Botin, Denis; Mapa, Ludmila Marotta; Schweinfurth, Holger; Sieber, Bastian; Wittenberg, Christopher; Palberg, Thomas
2017-05-28
Frequency domain super-heterodyne laser light scattering is utilized in a low angle integral measurement configuration to determine flow and diffusion in charged sphere suspensions showing moderate to strong multiple scattering. We introduce an empirical correction to subtract the multiple scattering background and isolate the singly scattered light. We demonstrate the excellent feasibility of this simple approach for turbid suspensions of transmittance T ≥ 0.4. We study the particle concentration dependence of the electro-kinetic mobility in low salt aqueous suspension over an extended concentration regime and observe a maximum at intermediate concentrations. We further use our scheme for measurements of the self-diffusion coefficients in the fluid samples in the absence or presence of shear, as well as in polycrystalline samples during crystallization and coarsening. We discuss the scope and limits of our approach as well as possible future applications.
Rayleigh scattering of twisted light by hydrogenlike ions
NASA Astrophysics Data System (ADS)
Peshkov, A. A.; Volotka, A. V.; Surzhykov, A.; Fritzsche, S.
2018-02-01
The elastic Rayleigh scattering of twisted light and, in particular, the polarization (transfer) of the scattered photons have been analyzed within the framework of second-order perturbation theory and Dirac's relativistic equation. Special attention was paid hereby to the scattering on three different atomic targets: single atoms, a mesoscopic (small) target, and a macroscopic (large) target, which are all centered with regard to the beam axis. Detailed calculations of the polarization Stokes parameters were performed for C5 + ions and for twisted Bessel beams. It is shown that the polarization of scattered photons is sensitive to the size of an atomic target and to the helicity, the opening angle, and the projection of the total angular momentum of the incident Bessel beam. These computations indicate more that the Stokes parameters of the (Rayleigh) scattered twisted light may significantly differ from their behavior for an incident plane-wave radiation.
Conservation law of angular momentum in helicity-dependent Raman and Rayleigh scattering
NASA Astrophysics Data System (ADS)
Tatsumi, Yuki; Kaneko, Tomoaki; Saito, Riichiro
2018-05-01
In first-order Raman scattering, helicity of circularly polarized incident light is either conserved or changed depending on the Raman modes. When the helicity of incident light changes in the scattered light, the angular momentum of a photon is transferred to the material. Here, we present the conservation law of pseudoangular momentum in the helicity-dependent Raman scattering for a N -fold (N =1 -4 ,6 ) rotational symmetry of a crystal. Furthermore, the conservation law of electron-phonon interaction is discussed by considering the vibration direction of a phonon that has the same or lower symmetry than the symmetry of the crystal, which is essential to allow the helicity change in Raman scattering in a highly symmetric material, such as graphene. We also discuss the conservation law of pseudoangular momentum in Rayleigh scattering and show that the helicity change is allowed only in the crystal with one- or twofold rotational symmetry.
Enhanced optical coupling and Raman scattering via microscopic interface engineering
NASA Astrophysics Data System (ADS)
Thompson, Jonathan V.; Hokr, Brett H.; Kim, Wihan; Ballmann, Charles W.; Applegate, Brian E.; Jo, Javier A.; Yamilov, Alexey; Cao, Hui; Scully, Marlan O.; Yakovlev, Vladislav V.
2017-11-01
Spontaneous Raman scattering is an extremely powerful tool for the remote detection and identification of various chemical materials. However, when those materials are contained within strongly scattering or turbid media, as is the case in many biological and security related systems, the sensitivity and range of Raman signal generation and detection is severely limited. Here, we demonstrate that through microscopic engineering of the optical interface, the optical coupling of light into a turbid material can be substantially enhanced. This improved coupling facilitates the enhancement of the Raman scattering signal generated by molecules within the medium. In particular, we detect at least two-orders of magnitude more spontaneous Raman scattering from a sample when the pump laser light is focused into a microscopic hole in the surface of the sample. Because this approach enhances both the interaction time and interaction region of the laser light within the material, its use will greatly improve the range and sensitivity of many spectroscopic techniques, including Raman scattering and fluorescence emission detection, inside highly scattering environments.
NASA Technical Reports Server (NTRS)
Register, D. F.; Trajmar, S.; Fineman, M. A.; Poe, R. T.; Csanak, G.; Jensen, S. W.
1983-01-01
Differential (in angle) electron scattering experiments on laser-excited Ba-138 1P were carried out at 30- and 100-eV impact energies. The laser light was linearly polarized and located in the scattering plane. The superelastic scattering signal was measured as a function of polarization direction of the laser light with respect to the scattering plane. It was found at low electron scattering angles that the superelastic scattering signal was asymmetric to reflection of the polarization vector with respect to the scattering plane. This is in contradiction with theoretical predictions. An attempt was made to pinpoint the reason for this observation, and a detailed investigation of the influence of experimental conditions on the superelastic scattering was undertaken. No explanation for the asymmetry has as yet been found.
Effects of temperature and salinity on light scattering by water
NASA Astrophysics Data System (ADS)
Zhang, Xiaodong; Hu, Lianbo
2010-04-01
A theoretical model on light scattering by water was developed from the thermodynamic principles and was used to evaluate the effects of temperature and salinity. The results agreed with the measurements by Morel within 1%. The scattering increases with salinity in a non-linear manner and the empirical linear model underestimate the scattering by seawater for S < 40 psu. Seawater also exhibits an 'anomalous' scattering behavior with a minimum occurring at 24.64 °C for pure water and this minimum increases with the salinity, reaching 27.49 °C at 40 psu.
Stray Light Analyis With The HP-41C/CV Calculator
NASA Astrophysics Data System (ADS)
Bamberg, Jack A.
1983-10-01
A stray radiation analysis program (nicknamed MINI-APART after its namesake: APART) suitable for use on the HP-41C/CV calculator is described. The program is ideally suited for quick estimates of stray light performance in well-baffled optical systems, which are limited by scatter from the first optical element. Critical path models are described, including single scatter, double scatter, diffraction-scatter, and thermal emission-scatter. Program use is illustrated, and several comparisons are made with the results obtained by the large stray radiation programs, GUERAP-3 and APART/PADE.
Wang, Tao; Jiang, Zhenfei; Ji, Xiaoling; Zhao, Daomu
2016-04-01
Spectral shifts and spectral switches of a polychromatic electromagnetic light wave on scattering from an anisotropic semisoft boundary medium are discussed. It is shown that both the property of the incident field and the character of the scattering medium play roles in the change of the spectrum of the far-zone scattered field. It is also shown that the distribution of the far-zone scattered spectrum, including the magnitude of the spectral shift and the direction at which the spectral switch occurs, is rotationally nonsymmetric.
NASA Technical Reports Server (NTRS)
Sun, Wenbo; Videnn, Gorden; Lin, Bing; Hu, Yongxiang
2007-01-01
Light scattering and transmission by rough surfaces are of considerable interest in a variety of applications including remote sensing and characterization of surfaces. In this work, the finite-difference time domain technique is applied to calculate the scattered and transmitted electromagnetic fields of an infinite periodic rough surface. The elements of Mueller matrix for scattered light are calculated by an integral of the near fields over a significant number of periods of the surface. The normalized Mueller matrix elements of the scattered light and the spatial distribution of the transmitted flux for a monolayer of micron-sized dielectric spheres on a silicon substrate are presented. The numerical results show that the nonzero Mueller matrix elements of the system of the monolayer of dielectric spheres on a silicon substrate have specific maxima at some scattering angles. These maxima may be used in characterization of the feature of the system. For light transmitted through the monolayer of spheres, our results show that the transmitted energy focuses around the ray passing through centers of the spheres. At other locations, the transmitted flux is very small. The technique also may be used to calculate the perturbance of the electromagnetic field due to the presence of an isolated structure on the substrate.
Blood cell counting and classification by nonflowing laser light scattering method
NASA Astrophysics Data System (ADS)
Yang, Ye; Zhang, Zhenxi; Yang, Xinhui; Jiang, Dazong; Yeo, Joon Hock
1999-11-01
A new non-flowing laser light scattering method for counting and classifying blood cells is presented. A linear charge- coupled device with 1024 elements is used to detect the scattered light intensity distribution of the blood cells. A pinhole plate is combined with the CCD to compete the focusing of the measurement system. An isotropic sphere is used to simulate the blood cell. Mie theory is used to describe the scattering of blood cells. In order to inverse the size distribution of blood cells from their scattered light intensity distribution, Powell method combined with precision punishment method is used as a dependent model method for measurement red blood cells and blood plates. Non-negative constraint least square method combined with Powell method and precision punishment method is used as an independent model for measuring white blood cells. The size distributions of white blood cells and red blood cells, and the mean diameter of red blood cells are measured by this method. White blood cells can be divided into three classes: lymphocytes, middle-sized cells and neutrocytes according to their sizes. And the number of blood cells in unit volume can also be measured by the linear dependence of blood cells concentration on scattered light intensity.
NASA Astrophysics Data System (ADS)
Toda, Sogo; Kato, Yuji; Kudo, Nobuki; Shimizu, Koichi
2017-04-01
For transillumination imaging of an animal body, we have attempted to suppress the scattering effect in a turbid medium. It is possible to restore the optical image before scattering using phase-conjugate light. We examined the effect of intensity information as well as the phase information for the restoration of the original light distribution. In an experimental analysis using animal tissue, the contributions of the phase- and the intensity-information to the image restoration through turbid medium were demonstrated.
1977-01-01
circumstances for determining the onset with light scattering is that in which the laser is so powerful and/or the detector so sensitive that the...sec Boltzmann’s constant 1.38 x 10~16 ergs/mole, wave number length of detector window latent heat of vaporisation mass flow rate of steam In...constant, distance from light scattering volume to detector S supersaturation ratio, p /p t time T local temperature of vapor T temperature in
A review of light-scattering techniques for the study of colloids in natural waters
Rees, T.F.
1987-01-01
In order to understand the movement of colloidal materials in natural waters, we first need to have a means of quantifying their physical characteristics. This paper reviews three techniques which utilize light-scattering phenomena to measure the translational diffusion coefficient, the rotational diffusion coefficient, and the electrophoretic mobility of colloids suspended in water. Primary emphasis is to provide sufficient theoretical detail so that hydrologists can evaluate the utility of photon correlation spectrometry, electrophoretic light scattering, and electric birefringence analysis. ?? 1987.
Gas temperature and density measurements based on spectrally resolved Rayleigh-Brillouin scattering
NASA Technical Reports Server (NTRS)
Seasholtz, Richard G.; Lock, James A.
1992-01-01
The use of molecular Rayleigh scattering for measurements of gas density and temperature is evaluated. The technique used is based on the measurement of the spectrum of the scattered light, where both temperature and density are determined from the spectral shape. Planar imaging of Rayleigh scattering from air using a laser light sheet is evaluated for ambient conditions. The Cramer-Rao lower bounds for the shot-noise limited density and temperature measurement uncertainties are calculated for an ideal optical spectrum analyzer and for a planar mirror Fabry-Perot interferometer used in a static, imaging mode. With this technique, a single image of the Rayleigh scattered light can be analyzed to obtain density (or pressure) and temperature. Experimental results are presented for planar measurements taken in a heated air stream.
Kim, K B; Shanyfelt, L M; Hahn, D W
2006-01-01
Dense-medium scattering is explored in the context of providing a quantitative measurement of turbidity, with specific application to corneal haze. A multiple-wavelength scattering technique is proposed to make use of two-color scattering response ratios, thereby providing a means for data normalization. A combination of measurements and simulations are reported to assess this technique, including light-scattering experiments for a range of polystyrene suspensions. Monte Carlo (MC) simulations were performed using a multiple-scattering algorithm based on full Mie scattering theory. The simulations were in excellent agreement with the polystyrene suspension experiments, thereby validating the MC model. The MC model was then used to simulate multiwavelength scattering in a corneal tissue model. Overall, the proposed multiwavelength scattering technique appears to be a feasible approach to quantify dense-medium scattering such as the manifestation of corneal haze, although more complex modeling of keratocyte scattering, and animal studies, are necessary.
Near specular scatter analysis method with a new goniophotometer
NASA Astrophysics Data System (ADS)
Meyen, Stephanie; Sutter, Florian; Heller, Peter
2014-09-01
The challenge of improving component quality and reducing cost has focused the attention of the solar thermal power industry on reliable component characterization methods. Since the reflector plays a key role in the energy conversion chain, the analysis of its reflectance properties has become a lively discussed issue in recent years. State of the art measurement instruments for specular reflectance do not give satisfying results, because they do not resolve sufficiently the near specular scatter of possible low cost mirror material candidates. The measurement of the BRDF offers a better solution than the traditional approach of placing a detector in the specular reflected beam path. However, due to the requirement of high angular resolution in the range of 1 mrad (0.057°) or better and the challenge of measuring high dynamic differences between the specular peak and the scatter signal, typical commercial scanning goniophotometers capable of this are rare. These instruments also face the disadvantages of impractically long acquisition times and, to reach the high angular resolution, occupy a large space (several meters side length). We have taken on the appealing idea of a parallel imaging goniophotometer and designed a prototype based on this principle. A mirrored ellipsoid is used to redirect the reflected light coming from a sample towards a camera with a fisheye lens. This way the complete light distribution is captured simultaneously. A key feature allows the distinction of the high intensity specular peak and the low intensity scatter. In this article we explain the prototype design and demonstrate its functionality based on comparison measurements done with a commercial scanning goniophotometer. We identify limitations related in part to the concept and in part to the specific prototype and suggest improvements. Finally we conclude that the concept is well suitable for the analysis of near specular scatter of mirror materials, although less adequate for the analysis of rough surfaces that require a full 180° view angle. Results obtained with this instrument are useful to evaluate the performance of a reflector material for a specific concentrating solar collector design and also serve in other applications that require near specular scatter analysis like degradation and soiling research.
Spectropolarimetric Imaging Observations
NASA Astrophysics Data System (ADS)
Bradley, Christine Lavella
The capability to map anthropogenic aerosol quantities and properties over land can provide significant insights for climate and environmental studies on global and regional scales. One of the primary challenges in aerosol information monitoring is separating two signals measured by downward-viewing airborne or spaceborne instruments: the light scattered from the aerosols and light reflected from the Earth's surface. In order to study the aerosols independently, the surface signal needs to be subtracted out from the measurements. Some observational modalities, such as multispectral and multiangle, do not provide enough information to uniquely define the Earth's directional reflectance properties for this task due to the high magnitude and inhomogeneity of albedo for land surface types. Polarization, however, can provide additional information to define surface reflection. To improve upon current measurement capabilities of aerosols over urban areas, Jet Propulsion Laboratory developed the Multiangle SpectroPolarimetric Imager (MSPI) that can accurately measure the Degree of Linear Polarization to 0.5%. In particular, data acquired by the ground-based prototype, GroundMSPI, is used for directional reflectance studies of outdoor surfaces in this dissertation. This work expands upon an existing model, the microfacet model, to characterize the polarized bidirectional reflectance distribution function (pBRDF) of surfaces and validate an assumption, the Spectral Invariance Hypothesis, on the surface pBRDF that is used in aerosol retrieval algorithms. The microfacet model is commonly used to represent the pBRDF of Earth's surface types, such as ocean and land. It represents a roughened surface comprised of randomly oriented facets that specularly reflect incoming light into the upward hemisphere. The analytic form of the pBRDF for this model assumes only a single reflection of light from the microfaceted surface. If the incoming illumination is unpolarized, as it is with natural light from the Sun, the reflected light is linearly polarized perpendicular to the plane that contains the illumination and view directions, the scattering plane. However, previous work has shown that manmade objects, such as asphalt and brick, show a polarization signature that differs from the single reflection microfacet model. Using the polarization ray-tracing (PRT) program POLARIS-M, a numerical calculation for the pBRDF is made for a roughened surface to account for multiple reflections that light can experience between microfacets. Results from this numerical PRT method shows rays that experience two or more reflections with the microfacet surface can be polarized at an orientation that differs from the analytical single reflection microfacet model. This PRT method is compared against GroundMSPI data of manmade surfaces. An assumption made regarding the pBRDF for this microfacet model is verified with GroundMSPI data of urban areas. This is known as the Spectral Invariance Hypothesis and asserts that the magnitude and shape of the polarized bidirectional reflectance factor (pBRF) is the same for all wavelengths. This simplifies the microfacet model by assuming some surface parameters such as the index of refraction are spectrally neutral. GroundMSPI acquires the pBRF for five prominent region types, asphalt, brick, cement, dirt, and grass, for day-long measurements on clear sky conditions. Over the course of each day, changing solar position in the sky provides a large range of scattering angles for this study. The pBRF is measured for the three polarimetric wavelengths of GroundMSPI, 470, 660, and 865nm, and the best fit slope of the spectral correlation is reported. This investigation shows agreement to the Spectral Invariance Hypothesis within 10% for all region types excluding grass. Grass measurements show a large mean deviation of 31.1%. This motivated an angle of linear polarization (AoLP) analysis of cotton crops to isolate single reflection cases, or specular reflections, from multiple scattering cases of light in vegetation. Results from this AoLP method show that specular reflections off the top surface of leaves follow the Spectral Invariance Hypothesis.
Photometric variability in earthshine observations.
Langford, Sally V; Wyithe, J Stuart B; Turner, Edwin L
2009-04-01
The identification of an extrasolar planet as Earth-like will depend on the detection of atmospheric signatures or surface non-uniformities. In this paper we present spatially unresolved flux light curves of Earth for the purpose of studying a prototype extrasolar terrestrial planet. Our monitoring of the photometric variability of earthshine revealed changes of up to 23% per hour in the brightness of Earth's scattered light at around 600 nm, due to the removal of specular reflection from the view of the Moon. This variability is accompanied by reddening of the spectrum and results from a change in surface properties across the continental boundary between the Indian Ocean and Africa's east coast. Our results based on earthshine monitoring indicate that specular reflection should provide a useful tool in determining the presence of liquid water on extrasolar planets via photometric observations.
NASA Astrophysics Data System (ADS)
Nursam, N. M.; Hidayat, J.; Shobih; Rosa, E. S.; Pranoto, L. M.
2018-04-01
The photoanode of dye-sensitized solar cells (DSSC) is typically composed of nanocrystalline titania (TiO2) layer that has been sensitized with light-absorbing dye molecules. Large portion of the light, however, could not be efficiently absorbed due to some physical reasons, such as TiO2 crystal size (typically 10-25 nm) that makes the photoanode remains partially transparent to the visible region in the solar spectrum. One of the ways to improve the light harvesting efficiency in DSSC could be achieved by employing an additional scattering layer over the TiO2 electron transport material. In this contribution, we evaluate the effect of light scattering properties on the performance of DSSC. Specifically, the light scattering properties provided from two different scattering materials, i.e. additional TiO2 scattering layer and zirconia (ZrO2) scattering layer, were compared. Both layers were deposited using screen printing technique under the same condition on top of 8 µm thick TiO2 photoanode layer. All samples subsequently received the same thermal annealing treatment at 500 °C and sensitized with ruthenium-based synthetic dyes. Our results revealed that the thickness of the scattering layer for both TiO2 and ZrO2 had a significant effect on the solar cell performance. The best photoconversion efficiency was achieved by samples that were coated with one screen-printing cycle, giving an overall efficiency of 3.50 % and 4.02% for TiO2 and ZrO2, respectively.
Coherent backscattering of light by complex random media of spherical scatterers: numerical solution
NASA Astrophysics Data System (ADS)
Muinonen, Karri
2004-07-01
Novel Monte Carlo techniques are described for the computation of reflection coefficient matrices for multiple scattering of light in plane-parallel random media of spherical scatterers. The present multiple scattering theory is composed of coherent backscattering and radiative transfer. In the radiative transfer part, the Stokes parameters of light escaping from the medium are updated at each scattering process in predefined angles of emergence. The scattering directions at each process are randomized using probability densities for the polar and azimuthal scattering angles: the former angle is generated using the single-scattering phase function, whereafter the latter follows from Kepler's equation. For spherical scatterers in the Rayleigh regime, randomization proceeds semi-analytically whereas, beyond that regime, cubic spline presentation of the scattering matrix is used for numerical computations. In the coherent backscattering part, the reciprocity of electromagnetic waves in the backscattering direction allows the renormalization of the reversely propagating waves, whereafter the scattering characteristics are computed in other directions. High orders of scattering (~10 000) can be treated because of the peculiar polarization characteristics of the reverse wave: after a number of scatterings, the polarization state of the reverse wave becomes independent of that of the incident wave, that is, it becomes fully dictated by the scatterings at the end of the reverse path. The coherent backscattering part depends on the single-scattering albedo in a non-monotonous way, the most pronounced signatures showing up for absorbing scatterers. The numerical results compare favourably to the literature results for nonabsorbing spherical scatterers both in and beyond the Rayleigh regime.
DOE Office of Scientific and Technical Information (OSTI.GOV)
Jacobson, C. M., E-mail: cjacobson@wisc.edu; Borchardt, M. T.; Den Hartog, D. J.
The Thomson scattering diagnostic on the Madison Symmetric Torus (MST) records excessive levels of stray Nd:YAG laser light. Stray light saturates the 1064 nm spectral channel in all polychromators, which prevents absolute electron density measurements via Rayleigh scattering calibration. Furthermore, stray light contaminates adjacent spectral channels for r/a ≥ 0.75, which renders the diagnostic unable to make electron temperature measurements at these radii. In situ measurements of stray light levels during a vacuum vessel vent are used to identify stray light sources and strategies for reduction of stray light levels. Numerical modeling using Zemax OpticStudio supports these measurements. The modelmore » of the vacuum vessel and diagnostic includes synthetic collection optics to enable direct comparison of measured and simulated stray light levels. Modeling produces qualitatively similar stray light distributions to MST measurements, and quantifies the mitigation effects of stray light mitigation strategies prior to implementation.« less
NASA Astrophysics Data System (ADS)
Tatsumi, Yuki; Saito, Riichiro
2018-03-01
Raman spectra of graphene and MoS2 are calculated for incident and scattered circularly polarized light. In the case of graphene, the well known G -band Raman spectra have a not well known property that the helicity of the incident circularly polarized light changes to another helicity in the scattered light. Using the electron-photon and electron-phonon matrix elements by first-principles calculation, we calculate resonant Raman spectra of graphene and MoS2 for circularly polarized light which are compared with recent experiments. The Raman intensity for circularly polarized light is relevant to optical valley polarization in the case of MoS2. We also discuss how the helicity-selection rule can be modified by applying stress to graphene.
A new dump system design for stray light reduction of Thomson scattering diagnostic system on EAST.
Xiao, Shumei; Zang, Qing; Han, Xiaofeng; Wang, Tengfei; Yu, Jin; Zhao, Junyu
2016-07-01
Thomson scattering (TS) diagnostic is an important diagnostic for measuring electron temperature and density during plasma discharge. However, the measurement of Thomson scattering signal is disturbed by the stray light easily. The stray light sources in the Experimental Advanced Superconducting Tokamak (EAST) TS diagnostic system were analyzed by a simulation model of the diagnostic system, and simulation results show that the dump system is the primary stray light source. Based on the optics theory and the simulation analysis, a novel dump system including an improved beam trap was proposed and installed. The measurement results indicate that the new dump system can reduce more than 60% of the stray light for the diagnostic system, and the influence of stray light on the error of measured density decreases.
NASA Astrophysics Data System (ADS)
Kutulakos, Kyros N.; O'Toole, Matthew
2015-03-01
Conventional cameras record all light falling on their sensor regardless of the path that light followed to get there. In this paper we give an overview of a new family of computational cameras that offers many more degrees of freedom. These cameras record just a fraction of the light coming from a controllable source, based on the actual 3D light path followed. Photos and live video captured this way offer an unconventional view of everyday scenes in which the effects of scattering, refraction and other phenomena can be selectively blocked or enhanced, visual structures that are too subtle to notice with the naked eye can become apparent, and object appearance can depend on depth. We give an overview of the basic theory behind these cameras and their DMD-based implementation, and discuss three applications: (1) live indirect-only imaging of complex everyday scenes, (2) reconstructing the 3D shape of scenes whose geometry or material properties make them hard or impossible to scan with conventional methods, and (3) acquiring time-of-flight images that are free of multi-path interference.
Dielectric Scattering Patterns for Efficient Light Trapping in Thin-Film Solar Cells.
van Lare, Claire; Lenzmann, Frank; Verschuuren, Marc A; Polman, Albert
2015-08-12
We demonstrate an effective light trapping geometry for thin-film solar cells that is composed of dielectric light scattering nanocavities at the interface between the metal back contact and the semiconductor absorber layer. The geometry is based on resonant Mie scattering. It avoids the Ohmic losses found in metallic (plasmonic) nanopatterns, and the dielectric scatterers are well compatible with nearly all types of thin-film solar cells, including cells produced using high temperature processes. The external quantum efficiency of thin-film a-Si:H solar cells grown on top of a nanopatterned Al-doped ZnO, made using soft imprint lithography, is strongly enhanced in the 550-800 nm spectral band by the dielectric nanoscatterers. Numerical simulations are in good agreement with experimental data and show that resonant light scattering from both the AZO nanostructures and the embedded Si nanostructures are important. The results are generic and can be applied on nearly all thin-film solar cells.
Dependence of the forward light scattering on the refractive index of particles
NASA Astrophysics Data System (ADS)
Guo, Lufang; Shen, Jianqi
2018-05-01
In particle sizing technique based on forward light scattering, the scattered light signal (SLS) is closely related to the relative refractive index (RRI) of the particles to the surrounding, especially when the particles are transparent (or weakly absorbent) and the particles are small in size. The interference between the diffraction (Diff) and the multiple internal reflections (MIR) of scattered light can lead to the oscillation of the SLS on RRI and the abnormal intervals, especially for narrowly-distributed small particle systems. This makes the inverse problem more difficult. In order to improve the inverse results, Tikhonov regularization algorithm with B-spline functions is proposed, in which the matrix element is calculated for a range of particle sizes instead using the mean particle diameter of size fractions. In this way, the influence of abnormal intervals on the inverse results can be eliminated. In addition, for measurements on narrowly distributed small particles, it is suggested to detect the SLS in a wider scattering angle to include more information.
Theory of scattering of electromagnetic waves of the microwave range in a turbid medium
NASA Astrophysics Data System (ADS)
Konstantinov, O. V.; Matveentsev, A. V.
2013-02-01
The coefficient of extinction of electromagnetic waves of the microwave range due to their scattering from clusters suspended in an amorphous medium and responsible for turbidity is calculated. Turbidity resembles the case when butter clusters transform water into milk. In the case under investigation, the clusters are conductors (metallic or semiconducting). The extinction coefficient is connected in a familiar way with the cross section of light scattering from an individual cluster. A new formula is derived for the light scattering cross section in the case when damping of oscillations of an electron is due only to spontaneous emission of light quanta. In this case, the resonant scattering cross section for light can be very large. It is shown that this can be observed only in a whisker nanocluster. In addition, the phonon energy on a whisker segment must be higher than the photon energy, which is close to the spacing between the electron energy levels in the cluster.
NASA Astrophysics Data System (ADS)
Nichman, Leonid; Järvinen, Emma; Dorsey, James; Connolly, Paul; Duplissy, Jonathan; Fuchs, Claudia; Ignatius, Karoliina; Sengupta, Kamalika; Stratmann, Frank; Möhler, Ottmar; Schnaiter, Martin; Gallagher, Martin
2017-09-01
Optical probes are frequently used for the detection of microphysical cloud particle properties such as liquid and ice phase, size and morphology. These properties can eventually influence the angular light scattering properties of cirrus clouds as well as the growth and accretion mechanisms of single cloud particles. In this study we compare four commonly used optical probes to examine their response to small cloud particles of different phase and asphericity. Cloud simulation experiments were conducted at the Cosmics Leaving OUtdoor Droplets (CLOUD) chamber at European Organisation for Nuclear Research (CERN). The chamber was operated in a series of multi-step adiabatic expansions to produce growth and sublimation of ice particles at super- and subsaturated ice conditions and for initial temperatures of -30, -40 and -50 °C. The experiments were performed for ice cloud formation via homogeneous ice nucleation. We report the optical observations of small ice particles in deep convection and in situ cirrus simulations. Ice crystal asphericity deduced from measurements of spatially resolved single particle light scattering patterns by the Particle Phase Discriminator mark 2 (PPD-2K, Karlsruhe edition) were compared with Cloud and Aerosol Spectrometer with Polarisation (CASPOL) measurements and image roundness captured by the 3View Cloud Particle Imager (3V-CPI). Averaged path light scattering properties of the simulated ice clouds were measured using the Scattering Intensity Measurements for the Optical detectioN of icE (SIMONE) and single particle scattering properties were measured by the CASPOL. We show the ambiguity of several optical measurements in ice fraction determination of homogeneously frozen ice in the case where sublimating quasi-spherical ice particles are present. Moreover, most of the instruments have difficulties of producing reliable ice fraction if small aspherical ice particles are present, and all of the instruments cannot separate perfectly spherical ice particles from supercooled droplets. Correlation analysis of bulk averaged path depolarisation measurements and single particle measurements of these clouds showed higher R2 values at high concentrations and small diameters, but these results require further confirmation. We find that none of these instruments were able to determine unambiguously the phase of the small particles. These results have implications for the interpretation of atmospheric measurements and parametrisations for modelling, particularly for low particle number concentration clouds.
Broadband light trapping in nanotextured thin film photovoltaic devices
NASA Astrophysics Data System (ADS)
Mennucci, Carlo; Muhammad, M. H.; Hameed, Mohamed Farhat O.; Mohamed, Shaimaa A.; Abdelkhalik, Mohamed S.; Obayya, S. S. A.; Buatier de Mongeot, Francesco
2018-07-01
Substrates with engineered roughness are studied with the aim of achieving broadband and omnidirectional photon harvesting in thin film devices. Light coupling across the interfaces of a photonic device is induced by uniaxial pseudo-periodic gratings formed in a self-organised fashion via de-focused ion beam sputtering (IBS). The optical properties of the textured interfaces are assessed both experimentally and numerically using finite difference time domain (FDTD) algorithm, quantitatively demonstrating the optimal geometries which favour broadband diffuse scattering of radiation across the Vis-NIR spectral range. Thin film amorphous silicon solar cells based on the nanostructured patterns have been numerically studied via FDTD to assess absorption enhancement in comparison to flat reference devices, finding a 25% increase of short-circuit current, in good agreement with the experiment. Similar light trapping experiments performed on prototypical solar cells employing a PTB7:PC61BM organic absorber, allow to extend the general validity of the results to a relevant class of materials in the view of photovoltaic applications.
Time-domain Brillouin scattering assisted by diffraction gratings
NASA Astrophysics Data System (ADS)
Matsuda, Osamu; Pezeril, Thomas; Chaban, Ievgeniia; Fujita, Kentaro; Gusev, Vitalyi
2018-02-01
Absorption of ultrashort laser pulses in a metallic grating deposited on a transparent sample launches coherent compression/dilatation acoustic pulses in directions of different orders of acoustic diffraction. Their propagation is detected by delayed laser pulses, which are also diffracted by the metallic grating, through the measurement of the transient intensity change of the first-order diffracted light. The obtained data contain multiple frequency components, which are interpreted by considering all possible angles for the Brillouin scattering of light achieved through multiplexing of the propagation directions of light and coherent sound by the metallic grating. The emitted acoustic field can be equivalently presented as a superposition of plane inhomogeneous acoustic waves, which constitute an acoustic diffraction grating for the probe light. Thus the obtained results can also be interpreted as a consequence of probe light diffraction by both metallic and acoustic gratings. The realized scheme of time-domain Brillouin scattering with metallic gratings operating in reflection mode provides access to wide range of acoustic frequencies from minimal to maximal possible values in a single experimental optical configuration for the directions of probe light incidence and scattered light detection. This is achieved by monitoring the backward and forward Brillouin scattering processes in parallel. Potential applications include measurements of the acoustic dispersion, simultaneous determination of sound velocity and optical refractive index, and evaluation of samples with a single direction of possible optical access.
A numerical experiment on light pollution from distant sources
NASA Astrophysics Data System (ADS)
Kocifaj, M.
2011-08-01
To predict the light pollution of the night-time sky realistically over any location or measuring point on the ground presents quite a difficult calculation task. Light pollution of the local atmosphere is caused by stray light, light loss or reflection of artificially illuminated ground objects or surfaces such as streets, advertisement boards or building interiors. Thus it depends on the size, shape, spatial distribution, radiative pattern and spectral characteristics of many neighbouring light sources. The actual state of the atmospheric environment and the orography of the surrounding terrain are also relevant. All of these factors together influence the spectral sky radiance/luminance in a complex manner. Knowledge of the directional behaviour of light pollution is especially important for the correct interpretation of astronomical observations. From a mathematical point of view, the light noise or veil luminance of a specific sky element is given by a superposition of scattered light beams. Theoretical models that simulate light pollution typically take into account all ground-based light sources, thus imposing great requirements on CPU and MEM. As shown in this paper, a contribution of distant sources to the light pollution might be essential under specific conditions of low turbidity and/or Garstang-like radiative patterns. To evaluate the convergence of the theoretical model, numerical experiments are made for different light sources, spectral bands and atmospheric conditions. It is shown that in the worst case the integration limit is approximately 100 km, but it can be significantly shortened for light sources with cosine-like radiative patterns.
Optical backscatter probe for sensing particulate in a combustion gas stream
Parks, James E; Partridge, William P
2013-05-28
A system for sensing particulate in a combustion gas stream is disclosed. The system transmits light into a combustion gas stream, and thereafter detects a portion of the transmitted light as scattered light in an amount corresponding to the amount of particulates in the emissions. Purge gas may be supplied adjacent the light supply and the detector to reduce particles in the emissions from coating or otherwise compromising the transmission of light into the emissions and recovery of scattered light from the emissions.
ERIC Educational Resources Information Center
Young, Andrew T.
1982-01-01
The correct usage of such terminology as "Rayleigh scattering,""Rayleigh lines,""Raman lines," and "Tyndall scattering" is resolved during an historical excursion through the physics of light-scattering by gas molecules. (Author/JN)
Yurkin, Maxim A; Semyanov, Konstantin A; Tarasov, Peter A; Chernyshev, Andrei V; Hoekstra, Alfons G; Maltsev, Valeri P
2005-09-01
Elastic light scattering by mature red blood cells (RBCs) was theoretically and experimentally analyzed by use of the discrete dipole approximation (DDA) and scanning flow cytometry (SFC), respectively. SFC permits measurement of the angular dependence of the light-scattering intensity (indicatrix) of single particles. A mature RBC is modeled as a biconcave disk in DDA simulations of light scattering. We have studied the effect of RBC orientation related to the direction of the light incident upon the indicatrix. Numerical calculations of indicatrices for several axis ratios and volumes of RBC have been carried out. Comparison of the simulated indicatrices and indicatrices measured by SFC showed good agreement, validating the biconcave disk model for a mature RBC. We simulated the light-scattering output signals from the SFC with the DDA for RBCs modeled as a disk-sphere and as an oblate spheroid. The biconcave disk, the disk-sphere, and the oblate spheroid models have been compared for two orientations, i.e., face-on and rim-on incidence, relative to the direction of the incident beam. Only the oblate spheroid model for rim-on incidence gives results similar to those of the rigorous biconcave disk model.
Measuring the Refractive Index of Bovine Corneal Stromal Cells Using Quantitative Phase Imaging
Gardner, Steven J.; White, Nick; Albon, Julie; Knupp, Carlo; Kamma-Lorger, Christina S.; Meek, Keith M.
2015-01-01
The cornea is the primary refractive lens in the eye and transmits >90% of incident visible light. It has been suggested that the development of postoperative corneal haze could be due to an increase in light scattering from activated corneal stromal cells. Quiescent keratocytes are thought to produce crystallins that match the refractive index of their cytoplasm to the surrounding extracellular material, reducing the amount of light scattering. To test this, we measured the refractive index (RI) of bovine corneal stromal cells, using quantitative phase imaging of live cells in vitro, together with confocal microscopy. The RI of quiescent keratocytes (RI = 1.381 ± 0.004) matched the surrounding matrix, thus supporting the hypothesis that keratocyte cytoplasm does not scatter light in the normal cornea. We also observed that the RI drops after keratocyte activation (RI = 1.365 ± 0.003), leading to a mismatch with the surrounding intercellular matrix. Theoretical scattering models showed that this mismatch would reduce light transmission in the cornea. We conclude that corneal transparency depends on the matching of refractive indices between quiescent keratocytes and the surrounding tissue, and that after surgery or wounding, the resulting RI mismatch between the activated cells and their surrounds significantly contributes to light scattering. PMID:26488650
Integrating Nephelometer Instrument Handbook
DOE Office of Scientific and Technical Information (OSTI.GOV)
Uin, J.
The Integrating Nephelometer (Figure 1) is an instrument that measures aerosol light scattering. It measures aerosol optical scattering properties by detecting (with a wide angular integration – from 7 to 170°) the light scattered by the aerosol and subtracting the light scattered by the carrier gas, the instrument walls and the background noise in the detector (zeroing). Zeroing is typically performed for 5 minutes every day at midnight UTC. The scattered light is split into red (700 nm), green (550 nm), and blue (450 nm) wavelengths and captured by three photomultiplier tubes. The instrument can measure total scatter as wellmore » as backscatter only (from 90 to 170°) (Heintzenberg and Charlson 1996; Anderson et al. 1996; Anderson and Ogren 1998; TSI 3563 2015) At ARM (Atmospheric Radiation Measurement), two identical Nephelometers are usually run in series with a sample relative humidity (RH) conditioner between them. This is possible because Nephelometer sampling is non-destructive and the sample can be passed on to another instrument. The sample RH conditioner scans through multiple RH values in cycles, treating the sample. This kind of setup allows to study how aerosol particles’ light scattering properties are affected by humidification (Anderson et al. 1996). For historical reasons, the two Nephelometers in this setup are labeled “wet” and “dry”, with the “dry” Nephelometer usually being the one before the conditioner and sampling ambient air (the names are switched for the MAOS measurement site due to the high RH of the ambient air).« less
Elastic light scattering from single cells: orientational dynamics in optical trap.
Watson, Dakota; Hagen, Norbert; Diver, Jonathan; Marchand, Philippe; Chachisvilis, Mirianas
2004-08-01
Light-scattering diagrams (phase functions) from single living cells and beads suspended in an optical trap were recorded with 30-ms time resolution. The intensity of the scattered light was recorded over an angular range of 0.5-179.5 degrees using an optical setup based on an elliptical mirror and rotating aperture. Experiments revealed that light-scattering diagrams from biological cells exhibit significant and complex time dependence. We have attributed this dependence to the cell's orientational dynamics within the trap. We have also used experimentally measured phase function information to calculate the time dependence of the optical radiation pressure force on the trapped particle and show how it changes depending on the orientation of the particle. Relevance of these experiments to potential improvement in the sensitivity of label-free flow cytometry is discussed.
Study of CCT varying by volume scattering diffuser with moving and rotating white light LED
NASA Astrophysics Data System (ADS)
Ma, Shih-Hsin; Chen, Liang-Shiun; Huang, Wen-Chao
2014-09-01
In this study, the corrected color temperature (CCT) of white light, which originates from a white light LED (WLLED) and passes through a volume-scattering diffuser (VSD), is investigated. The VSD with thickness of 2mm is fabricated by mixing the 2um-sized PMMA scattering particles and the epoxy glue with different concentration values. Moreover, in order to understand the influences of the illuminated area and the scattering path of VSD on CCT values, the bulletheaded and lambertian-type WLLEDs are assembled for different positions and distinct orientations along the optical axis in a black cavity. A detailed comparison between results regarding the white light with and without passing through the VSD is offered. The results of this research will help to improve the colorful consistency of the LED lamps which use diffusers.