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Sample records for lutjanus perciformes lutjanidae

  1. Two new gonad-infecting Philometra species (Nematoda: Philometridae) from the marine fish Lutjanus vitta (Perciformes: Lutjanidae) off New Caledonia.

    PubMed

    Moravec, Frantisek; Justine, Jean-Lou

    2011-11-01

    Two new gonad-infecting species of Philometra Costa, 1845 (Philometridae), P. brevicollis sp. n. and P. mira sp. n., are described from the ovary of the brownstripe red snapper, Lutjanus vitta (Quoy et Gaimard) (Lutjanidae, Perciformes), from the South Pacific Ocean off the coast of New Caledonia. Based on light and scanning electron microscopy examination, these new nematodes are compared with all other gonad-infecting species of Philometra. The former species (males and mature and gravid females available) mainly differs from its congeners in the length of males (5.39-5.92 mm), equally long spicules (279-312 microm), and the gubernaculum (151-168 microm), in that the distal end of the gubernaculum bears dorsolateral transverse lamella-like structures, and in the structure of the male caudal end. The latter species (only males available) is characterized by the length of males (3.47-3.66 mm), equally long spicules (96-105 microm), and the gubernaculum (78-84 microm), the absence of a dorsal reflected barb or lamella-like structures on the distal end of gubernaculum, and by the structure of the male caudal end. Judging from the occurrence in the host, P. brevicollis is the main philometrid parasitizing the gonads of L. vitta, in which it can reproduce, whereas this fish probably serves as only the paradefinitive host for P. mira. Both P. brevicollis and P. mira are the first nominal species of Philometra described from fishes of the family Lutjanidae.

  2. [Reproductive biology of Lutjanus guttatus (Perciformes: Lutjanidae) in Utria National Park, Colombian Pacific].

    PubMed

    Correa-Herrera, Tatiana; Jiménez-Segura, Luz Fernanda

    2013-06-01

    Protected areas are important for natural population conservation since they work as refuge, feeding and breeding areas, where specimens should be exempt from human pressure. The generation of better criteria for management decisions and conservation of fishery resources, is based on the reproductive aspects of species that support fishing activities, since this information is related to the abundance, size and frequency of capture. With this aim, the reproductive biology of the spotted snapper Lutjanus guttatus was studied from April 2008 and February 2009 at the Utría National Park, Colombian Pacific. For this, we analyzed the volume of catch, size structure, sex ratio, fecundity, maturity size, breeding areas and seasons (n = 278), of daily landings of 21 units of artisanal fisheries in ten fishing grounds in the Park. Form all landings, we evaluated a total of 4319 individuals belonging to 84 species. Based on the number of individuals, Lutjanus guttatus ranked third in catches representing 6.4% (278 individuals), and 16th with 1.8% (95.79kg), based on catch biomass. The average weight was 0.34 kg +/- 0.25 kg, while 29 cm +/- 6.4 cm for total length. The total length-weight relationship had the best fit (Kruskal-Wallis, p < 0.05, n = 272) with the equation P(T) = 0.00000885* LT3.09. The occurrence of mature fish and high condition factors suggested a spawning season in June, September and October in sandy and rocky shores. This species showed an asynchronical gonadal development, with a mean sexual maturity size estimated in 23.5cm total length, and an absolute fecundity of 156 253.11 oocytes (mode of 4 microm diameter). We concluded that L. guttatus medium sizes observed indicated a fishing pressure on small size specimens (the minimum size being 18cm); thus, we recommend the implementation of minimum catch sizes based on the criterion of size at maturity LT100 (25.5 cm) and to apply seasonal fishing closures during the highest reproductive activity (June

  3. Oocyte development and fecundity type of the Brazilian Snapper Lutjanus alexandrei Moura & Lindeman, 2007 (Perciformes: Lutjanidae).

    PubMed

    Fernandes, C A F; Oliveira, P G V; Oliveira, C H B; Hazin, F H V; Travassos, P

    2016-02-01

    Lutjanid species exhibit multiple spawning behaviour during an extended spawning season in warm months, asynchronous oocyte development and indeterminate fecundity. Although early studies have contributed to knowledge of the reproductive cycle of many species within the group, they have not considered aspects about the number of cortical alveoli oocyte stage throughout maturity phases along spawning season. The latter aspect is also considered very important to confirm indeterminate fecundity hypothesis. In the present study, were analyzed 154 Brazilian snapper Lutjanus alexandrei female gonads obtained from artisanal fisheries in Pernambuco State (Brazil) between October 2010 and March 2011. Were measured oocyte size frequency distribution for maturity phases (developing, spawning capable and actively spawning), and oocyte development stage (unyolked oocytes, cortical alveoli, primary, secondary and tertiary vitellogenic oocytes and hydrated oocytes), and also the oocyte stage frequency during spawning season. The frequency of cortical alveoli oocyte stage was constantly found in the spawning period (>37%), showing slight variation throughout maturity phases. The absence of gap in the oocyte size frequency distribution between primary and secondary oocyte growth stages during spawning season is a strong indicator of continuous oocyte recruitment from reserve stocks. In addition, co-occurrence of tertiary vitellogenic oocytes, hydrated oocytes, post-ovulatory follicles and yellow-brown bodies in the histological sections of ovaries reinforce indeterminate fecundity hypothesis.

  4. [Reproductive biology of the golden snapper Lutjanus inermis (Perciformes: Lutjanidae) in the central Mexican Pacific].

    PubMed

    Lucano-Ramírez, Gabriela; Ruiz-Ramírez, Salvador; González-Sansón, Gaspar; Ceballos-Vázquez, Bertha Patricia

    2012-03-01

    Reproductive aspects and information on the effect of fishery activities over this species stock are scarce, despite the imperative need of effective protection and resource management programs. To support the possible establishment of closed seasons and catch levels for the area, our study aimed to evaluate the spawning season of the Golden snapper (Lutjanus inermis). For this, fish caught by artisanal fisheries in the Navidad, Tenacatita and Chamela bays (Jalisco, Mexico) were sampled monthly from 1999-2008, and analyzed sex ratios, spawning season and size at first maturity. A total of 311 (53.1%) females and 275 (46.9%) males were caught, and the observed sex ratio was 1:0.88. The total length range was 14.4-37.0cm and total weight range from 48-575g. The length at first maturity was 23.9cm TL for females and 23.6cm TL for males. The gonadosomatic index showed two principal reproductive periods in the year (February-April and September-November), when the largest number of mature gonads were observed. Ovaries were of asynchronic development type and seven oocyte development stages were identified. Testicles showed lobular development and spermatozoa were observed in the center of the cysts and in the collecting ducts. It is important to mention that our results support the current suitable exploitation of this species in the area.

  5. Helminth parasites of the red snapper, Lutjanus campechanus (Perciformes: Lutjanidae) from the reef Santiaguillo, Veracruz, Mexico.

    PubMed

    Montoya-Mendoza, Jesús; Jiménez-Badillo, Lourdes; Salgado-Maldonado, Guillermo; Mendoza-Franco, Edgar F

    2014-12-01

    A total of 21 helminth species were recovered from 52 specimens of red snapper, Lutjanus campechanus , captured in the reef Santiaguillo, Parque Nacional Sistema Arrecifal Veracruzano, State of Veracruz, in the Southern Gulf of Mexico. These helminths included 9 trematodes (7 adults and 2 metacercariae), 4 nematodes (3 adults and 1 larva), 4 acanthocephalans (1 adult and 3 juvenile), 2 cestodes (both larvae), and 2 monogeneans. Sixteen of the 21 species are new host records; 7 are common species with a prevalence >40% and mean intensity >4.1. The monogenean Euryhaliotrema tubocirrus was the most-prevalent parasite with a prevalence of 78.8%, followed by the intestinal plerocercoids of Tetraphyllidea with a prevalence of 59.6%. The richness (S = 21), and diversity (Shannon index H = 2.17) in the component community, as well as in the infracommunity level (S = 5.1 ± 2.2, H = 0.92 ± 0.4), was similar to those found in other marine fish of temperate and tropical latitudes. The present study suggests that the composition of the parasite community is associated with the host feeding habits because 18/21 of the recorded species are trophically transmitted.

  6. Cardicola beveridgei n. sp. (Digenea: Aporocotylidae) from the mangrove jack, Lutjanus argentimaculatus (Perciformes: Lutjanidae), and C. bullardi n. sp. from the Australian spotted mackerel, Scomberomorus munroi (Perciformes: Scombridae), from the northern Great Barrier Reef.

    PubMed

    Nolan, Matthew J; Miller, Terrence L; Cutmore, Scott C; Cantacessi, Cinzia; Cribb, Thomas H

    2014-10-01

    Cardicola Short, 1953 is a genus of the Aporocotylidae Odhner, 1912 (Digenea), with 25 currently recognised species described from 32 species of Perciformes and Mugiliformes fishes around the world, including eight species from the Great Barrier Reef. Here, we describe two new species from this region, namely Cardicola beveridgei n. sp. from the ventricle and atrium of the mangrove jack, Lutjanus argentimaculatus (Forsskål) (Perciformes: Lutjanidae), and Cardicola bullardi n. sp. from the ventricle of the Australian spotted mackerel, Scomberomorus munroi Collette & Russo (Perciformes: Scombridae), from off Lizard Island, Queensland, Australia. These two new species are most easily distinguished from the 25 current members of Cardicola in having the combination of i) a spinous oral sucker, ii) an anteriorly intercaecal ovary, iii) a uterus that extends anteriorly from the oötype, iv) the number of spines per ventrolateral transverse row, and in v) body size and the length/width ratio, vi) the oesophagus and caecal length(s) relative to body total length, vii) the length of the posterior caeca relative to the anterior pair, viii) the testis length/width ratio and its total size relative to that of the body, ix) the postovarian field as a percentage of body length, and x) egg size. In addition, C. beveridgei n. sp. is further differentiated by possessing a female genital pore that opens anterodextral to the male pore while C. bullardi n. sp. differs further in possessing a testis that is almost entirely intercaecal and does not extend anteriorly to the level of the intestinal bifurcation. Employing genetic analysis of ITS2 rDNA sequence data, representing these species and a further 13 recognised and three putative species of Cardicola, we were able to unequivocally confirm these specimens as distinct (9-22% different over 420 nucleotide positions). Distance analysis of ITS2 showed that i) species of Cardicola from the Siganidae formed a monophyletic clade, to the

  7. Mitogenomes of genus Pristipomoides, Lutjanus and Pterocaesio confirm Caesionidae nests in Lutjanidae.

    PubMed

    Guo, Yusong; Bai, Qin; Yan, Tao; Wang, Zhongduo; Liu, Chuwu

    2016-05-01

    In this study, the complete mitochondrial genome of Goldbanded jobfish (Perciformes: Lutjanidae: Etelinae: Pristipomoides multidens) was sequenced using long-PCR amplification (Accession No.: KF430626). The mitochondrial genome is highly homologous to those of other teleosts. Consensus tree based on phylogeny analysis of nucleotide sequences using the protein-coding regions combined sequences (apart from ND6) in 11 species supports the fact that Pristipomoides multidens of Etelinae and the cluster of Subfamily Lutjaninae remain the sister relationship, consistent with the traditional taxonomy, while the fusiliers (Pterocaesio tile) of family Caesionidae nested within the genus Lutjanus of family Lutjanidae and showed more closely relationship with the non-reddish snappers cluster including L. russellii and L. argentimaculatus. Conclusively, this mitogenomic phylogeny indicates that the Caesionidae is a synonym of family Lutjanidae. Given the morphologic and feeding diversity, we hypothesize that the variation of feeding systems of genus Lutjanus may drive the speciation of snappers and fusiliers.

  8. Lack of Spatial Subdivision for the Snapper Lutjanus purpureus (LutjanidaePerciformes) from Southwest Atlantic Based on Multi-Locus Analyses

    PubMed Central

    Sampaio, Iracilda; Schneider, Horacio

    2016-01-01

    The Caribbean snapper Lutjanus purpureus is a marine species fish commonly found associated with rocky seabeds and is widely distributed along of Western Atlantic. Data on stock delineation and stock recognition are essential for establishing conservation measures for commercially fished species. However, few studies have investigated the population genetic structure of this economically valuable species, and previous studies (based on only a portion of the mitochondrial DNA) provide an incomplete picture. The present study used a multi-locus approach (12 segments of mitochondrial and nuclear DNA) to elucidate the levels of genetic diversity and genetic connectivity of L. purpureus populations and their demographic history. L. purpureus has high levels of genetic diversity, which probably implies in high effective population sizes values for the species. The data show that this species is genetically homogeneous throughout the geographic region analyzed, most likely as a result of dispersal during larval phase. Regarding demographic history, a historical population growth event occurred, likely due to sea level changes during the Pleistocene. PMID:27556738

  9. Three new gonad-infecting species of Philometra (Nematoda: Philometridae) parasitic in Lutjanus spp. (Lutjanidae) in the northern Gulf of Mexico off Florida, USA.

    PubMed

    Moravec, Frantisek; Bakenhaster, Micah; Fajer-Avila, Emma J

    2014-08-01

    Based on light and scanning electron microscopical studies, three new gonad-infecting species of Philometra Costa, 1845 (Nematoda: Philometridae) are described from marine fishes of the genus Lutjanus Bloch (Perciformes: Lutjanidae) in the northern Gulf of Mexico: P. longispicula sp. n. from the ovary of the northern red snapper L. campechanus (Poey) (type host) and silk snapper L. vivanus (Cuvier); P. latispicula sp. n. from the ovary and rarely testes of the grey snapper L. griseus (Linnaeus); and P. synagridis sp. n. (only males available) from the ovary of the lane snapper Lutjanus synagris (Linnaeus). These species are mainly characterised by the lengths of spicules (378-690 microm, 135-144 microm and 186-219 microm, respectively) and spicule shapes, structure of the distal portion of the gubernaculum and the structure of the male caudal end. These are the first valid, nominal species of gonad-infecting philometrids reported from fishes of the family Lutjanidae in the western Atlantic region.

  10. [Reproduction and growth of the fish Lutjanus guttatus (Pisces: Lutjanidae) in the Gulf of Nicoya, Costa Rica].

    PubMed

    Rojas, Rosa L Soto; Mejía-Arana, Fernando; Palacios, J A; Hiramatsu, Kazuhito

    2009-01-01

    Reproduction and growth of the fish Lutjanus guttatus (Pisces: Lutjanidae) in the Gulf of Nicoya, Costa Rica. The fish Lutjanus guttatus is important in the fisheries of Golfo de Nicoya, Costa Rica where they are captured with varied gear mainly by the artisanal fleet. We measured specimens from the commercial catch of 2002-2006. Gonadal state was determined macroscopically and age with otoliths The total length-total weight relationship was y = 0.0236x(2.8153) and total length-eviscerated weight y = 0.0216x(2.8129). Simplified relationships were y = 0.0173x3 and y = 0.0162x3. There is year-round reproduction with peaks in March (dry season) and September (rainy season). Male-female sexual ratio was 1:1. The age-length key for the gulf shows availability until the 6 years of age. The von Bertalanffy growth curve is L(t) = 65,9 (1-e(-0.13(t+2,66))).

  11. Molecular protocol for authentication of snappers (Lutjanidae-Perciformes) based on multiplex PCR.

    PubMed

    Veneza, Ivana; da Silva, Raimundo; Sampaio, Iracilda; Schneider, Horacio; Gomes, Grazielle

    2017-10-01

    Fraud involving fish products is regularly reported and investigated using genetic methods. However, no such approach has been applied to snappers, despite the commercial relevance of these fish. This study proposes an authentication protocol with multiplex PCR for three species of snappers (Lutjanus purpureus, L. synagris, and Ocyurus chrysurus). The protocol yielded a distinct triple-banding pattern for L. purpureus, whereas L. synagris and O. chrysurus showed a double banding pattern of different sizes, thereby allowing differentiation of the three species. The protocol was validated using fillets labeled as snapper or "Pargo" in Brazil, previously identified using DNA sequencing, amongst which substitution with Rhomboplites aurorubens was detected. When subjected to the new protocol, banding characteristic of L. purpureus were detected whilst R. aurorubens generated only the control band. Our study provides a practical tool for investigating substitutions and might assist in quality control and increase food safety for consumers. Copyright © 2017. Published by Elsevier Ltd.

  12. [Feeding habits of Lutjanus guttatus (Pisces: Lutjanidae) at Los Cóbanos and Puerto La Libertad, El Salvador].

    PubMed

    Rodrigo Rojas, José; Maravilla, Erick; Chicas, Francisco

    2004-03-01

    Abstract: A total of 175 spotted snapper Lutjanus guttatus were collected monthly in the Los Cobanos and Puerto La Libertad, El Salvador, from January to December 2000 to determine its feeding habits. The fishes studied ranged 9.8 - 58.0 cm in total length. Were collected using push-net and hook and line. This snapper is a bentonic opportunistic carnivorous predator. The total biomass of the stomach contents was 260.5 g. Crustaceans (Squillidae, Portunidae, Dynomenidae. Penaeidae, Sicyoniidae, Callianassidae), were the most abundant group: they accounted for 50.4% of the total biomass. Numerically, Portunus asper was the most abundant prey. Ontogenic differences were observed in the diet. In juveniles (16 cm TL). at any time of the year, the most frequent and abundant components were crustaceans and in adults were crustaceans, fishes and mollusks. The relative importance of different components of the diet was assessed with two indexes that combine. in different ways. percentage frequency of occurrence, percentage number and percentage weight of prey categories. The commercial use this resource and the absence of management strategies are discussed.

  13. A new species of Dentiphilometra (Nematoda: Philometridae) from the musculature of the gray snapper Lutjanus griseus (osteichthyes) off the Caribbean coast of Mexico.

    PubMed

    González-Solís, David; Moravec, Frantisek; Paredes, Vielka M Tuz

    2007-10-01

    A new nematode, Dentiphilometra lutjani n. sp. (Philometridae), is described from gravid females (the male is unknown) collected from the body musculature of the marine perciform fish gray snapper, Lutjanus griseus (Lutjanidae), from the Bay of Chetumal and southern coast of Quintana Roo, off the Caribbean coast of Mexico. The new species differs from the only other congener, Dentiphilometra monopteri, from the swamp eel Monopterus albus in China, mainly in the body length of gravid female (15.40-53.21 mm), the shape of the posterior body end (not markedly narrowed, with low caudal projections), the esophageal gland (maximum width near its posterior end), and the length (344-483 microm) of larvae from the uterus; both species also differ in their host types (marine perciform fish vs. freshwater swamp eel) and geographical distribution (Mexico vs. China).

  14. [Osteological development of the vertebral column and caudal complex of Lujanus guttatus (Perciformes: Lutjanidae) larvae under rearing conditions].

    PubMed

    Rodríguez-Ibarra, Luz Estela; Abdo-de la Parra, María Isabel; Aguilar-Zárate, Gabriela; Valasco-Blanco, Gabriela; Ibarra-Castro, Leonardo

    2015-03-01

    The spotted rose snapper (Lutjanus guttatus) is an important commercial species in Mexico with good culture potential. The osteological study at early stages in this species is an important tool to confirm normal bone structure and for the detection of malformations that may occur during early development. This study was carried out in order to evaluate and describe the normal osteological development of the vertebral column and caudal complex of this species grown under controlled conditions. For this, a total of 540 larvae of L. guttatus, between 2.1 and 17.5 mm of total length (TL), were cultured during 36 days; culture conditions were 28 degrees C, 5.74 mg/L oxygen and 32.2 ups salinity with standard feeding rates. To detect growth changes, a sample of 15 organisms was daily taken from day one until day 36 of post-hatch (DPH). Samples were processed following standard techniques of clearing, and cartilage (alcian blue) and bone staining (alizarin red). Results showed that the vertebral column is composed of ten vertebrae in the abdominal region, and 14 vertebrae including the urostyle in the caudal region. The development of the axial skeleton starts with the neural arches and haemal arches at 3.8 mm TL. Caudal elements such as the hypurals and parahypural began to develop at 4.1 mm TL. Pre-flexion and flexion of the notochord and the formation of all hypurals were observed between 5.3 and 5.8 mm TL. Ossification of the vertebrae in the abdominal region and in some neural arches initiated at 9.5mm TL. In the caudal region, all the neural and haemal arches ossified at 10.2 mm TL. All the abdominal vertebrae and their respective neural arches and parapophyses ossified at 11.2 mm TL, while the elements of the caudal complex that ossified were the hypurals, parahypurals and modified haemal spines. All caudal fm rays, 12 neural spines and 3 haemal arches were ossified by 15.5 mm. The complete ossification process of this specie under laboratory culture conditions

  15. Candidatus Renichlamydia lutjani, a Gram-negative bacterium in internal organs of blue-striped snapper Lutjanus kasmira from Hawaii.

    PubMed

    Corsaro, Daniele; Work, Thierry M

    2012-04-26

    The blue-striped snapper Lutjanus kasmira (Perciformes, Lutjanidae) are cosmopolitan in the Indo-Pacific but were introduced into Oahu, Hawaii, USA, in the 1950s and have since colonized most of the archipelago. Studies of microparasites in blue-striped snappers from Hawaii revealed chlamydia-like organisms (CLO) infecting the spleen and kidney, characterized by intracellular basophilic granular inclusions containing Gram-negative and Gimenez-positive bacteria similar in appearance to epitheliocysts when seen under light microscopy. We provide molecular evidence that CLO are a new member of Chlamydiae, i.e. Candidatus Renichlamydia lutjani, that represents the first reported case of chlamydial infection in organs other than the gill in fishes.

  16. [Feeding habits and trophic ecology of the fish Lutjanus griseus (Pisces: Lutjanidae) associated with submerged vegetation in Laguna de Términos, Campeche, Mexico].

    PubMed

    Guevara, Emma; Alvarez, Hernán; Mascaró, Maite; Rosas, Carlos; Sánchez, Alberto

    2007-01-01

    In Campeche, Mexico, the Laguna de Términos has Thalassia testudinum beds inhabited by the grey snapper, Lutjanus griseus (Linnaeus 1758). Along one year, we collected 994 individuals and 672 had food in their stomachs; we recorded number, weight, and frequency oftrophic groups, and the index of relative importance. Dominant food components were Farfantepenaeus duorarum and Palemonetes octaviae, Eucinostomus gula (Cuvier 1830) and Libinia dubia. Salinity and temperature were related to the number of individuals collected and with greater fish consumption by large L. griseus. The trophic niche breadth index was calculated for six size-classes of fish. The smallest and the largest fish had the lowest index values, whereas medium-size snappers had higher values. This snapper has a preference for habitats with submerged vegetation. The percentage of fish with food in their stomachs was higher during dark hours, providing evidence of the nocturnal habits of this fish.

  17. First report of gastrocotylinean post-oncomiracidia (Platyhelminthes: Monogenoidea: Heteronchoinea) on gills of flyingfish (Exocoetidae), snapper (Lutjanidae), dolphinfish (Coryphaenidae), and amberjack (Carangidae) from the Gulf of Mexico: decoy hosts and the dilution effect.

    PubMed

    Kritsky, Delane C; Bullard, Stephen A; Bakenhaster, Micah D

    2011-09-01

    Larvae, identified as post-oncomiracidia of the suborder Gastrocotylinea (Monogenoidea), were collected from formalin-fixed gills excised from six species of marine fishes captured from the Gulf of Mexico off Mississippi and Florida: common dolphinfish, Coryphaena hippurus and pompano dolphinfish, Coryphaena equiselis (both Perciformes, Coryphaenidae); gray snapper, Lutjanus griseus (Perciformes, Lutjanidae); greater amberjack, Seriola dumerili (Perciformes, Carangidae); and Atlantic flyingfish, Cheilopogon melanurus and sailfin flyingfish, Parexocoetus hillianus (both Beloniformes and Exocoetidae). Based on a combination of diagnostic morphological features, the specimens were divided into two basic forms, each of which was further subdivided into two morphotypes. No gastrocotylinean post-oncomiracidium had been reported previously from these hosts. Of the six host species, only C. hippurus serves as a host (unconfirmed) for the adult of a gastrocotylinean species, suggesting that the recorded fishes from the Gulf of Mexico comprise dead-end hosts acting as decoys for the oncomiracidia. These comparatively non-susceptible "decoy hosts" apparently dilute the susceptible fish-host population and by intercepting infective larvae (oncomiracidia) decrease the abundance of parasites on their typical hosts.

  18. Life-stages, exploitation status and habitat use of Lutjanus goreensis (Perciformes: Lutjanidae) in coastal marine environments of Lagos, SW Nigeria.

    PubMed

    Kafayat, A Fakoya; Martins, A Anetekhai; Shehu, L Akintola; Abdulwakil, O Sabal; Abass, Mikhail A

    2015-03-01

    The Gorean snapper, Lutanus goreensis is an important component of artisanal fisheries and trawl landings in the Gulf of Guinea. Despite its economic importance, there is a dearth of information on size structure and life history strategies of the species. Therefore, the objectives of this study were to provide baseline data on the life stages, exploitation status and habitat use for the species in Nigeria. Monthly samples were obtained from artisanal and trawl catches in Five Cowrie Creek and Lagos coastal waters between December 2008 and December 2010, respectively. Length-frequency distributions of the fishes caught were analysed to provide preliminary information on mean and modal lengths at capture and life-history strategies based on habitat use and estuarine-dependency for L. goreensis. A total of 822 specimens of L. goreensis were collected from Five Cowrie Creek while 377 specimens were collected from Lagos coastal waters. Total length varied between 7.90-34.90 cm for creek samples and from 21.90-56.10 cm for marine samples. Length-frequency histograms showed polymodal size distributions in creek and marine samples. Length-frequency distributions of L. goreensis showed a high abundance ofjuveniles (<20 cm) and sub-adults (20-35 cm) which accounted for 84.1% and 68.4% of creek and marine samples examined, respectively. For the creek samples, fish in modal length class of 13.00-13.99 cm were the most exploited while in the marine samples, length classes of 29.00-30.99 cm and 31.00-32.99cm constituted the most frequently exploited fishes. Increase in total lengths from the creek (mean +/- SD; 16.19 +/- 3.73 cm) to the marine habitat samples (32.89 +/- 6.14 cm) indicated ontogenetic shift in habitat use. Occurrence of a predominant juvenile population in Five Cowrie Creek by L. goreensis suggests estuarine-dependency and is indicative of a temporary juvenile habitat or a migratory corridor. In conclusion, data from the presently reported study and previous studies demonstrated that juvenile L. goreensis displays estuarine dependency and habitat flexibility. Hence, this underscores the importance of preserving estuarine environments as essential fish habitats to prevent overfishing. The study also concludes that the species is vulnerable to recruitment overfishing in the marine environment especially as a consequence of shrimping. Consequently, it advocates for ban on all fishing activities during peak spawning periods in breeding grounds and shrimp ground assemblage.

  19. Validity of a blue stripe snapper, Lutjanus octolineatus (Cuvier 1828) and a related species, L. bengalensis (Bloch 1790) with a new species (Pisces; Lutjanidae) from the Arabian Sea.

    PubMed

    Iwatsuki, Yukio; Al-Mamry, Juma M; Heemstra, Phillip C

    2016-04-07

    Lutjanus octolineatus (Cuvier 1828), previously considered a junior synonym of Lutjanus bengalensis (Bloch 1790), is shown to be a valid species and lectotypes are designated. Both species are redescribed. The two species have overlapping distributions in the Indian Ocean, but are clearly separable by different dorsal-fin spine counts, blue-striped pattern on the body and the presence or absence of a subocular extension of cheek scales. Lutjanus octovittata (Valenciennes 1830), formerly assigned to synonymy of L. bengalensis, is considered a junior synonym of L. octolineatus based on examination of the holotype. Lutjanus sapphirolineatus n. sp., a species formerly misidentified as L. bengalensis, is described based on 10 specimens from Oman and Somalia. The new species differs from the three species above by a combination of different characters. Analysis of the mitochondrial cytochrome c oxidase subunit 1 (CO1, 603 bp) genetic marker, also strongly supports the validity of each species of the blue-striped snapper complex as distinct.

  20. Complete mitogenome sequences of the pacific red snapper (Lutjanus peru) and the spotted rose snapper (Lutjanus gutattus).

    PubMed

    Bayona-Vásquez, Natalia Juliana; Hernández-Álvarez, Cristóbal Alejandro; Glenn, Travis; Domínguez-Domínguez, Omar; Uribe-Alcocer, Manuel; Díaz-Jaimes, Píndaro

    2017-03-01

    The structure of the mitochondrial genome for the Pacific red snapper, Lutjanus peru, and the spotted rose snapper, Lutjanus gutattus, of specimens collected in the eastern Pacific is similar to the reported for other teleosts and shares the same configuration with other members of the family Lutjanidae. It has a total length of 16 502 and 16 508 base pairs (bp) for Lutjanus peru and L. gutattus, respectively; on average the base composition was A (27.9%), T (24.8%) C (30.9%), and G (16.4%), containing 13 protein-coding genes, 2 rRNA genes, and 22 tRNA genes; and the leucine (Leu) tRNA is duplicated.

  1. Ethnobiology of snappers (Lutjanidae): target species and suggestions for management

    PubMed Central

    2011-01-01

    In this study, we sought to investigate the biology (diet and reproduction) and ethnobiology (fishers knowledge and fishing spots used to catch snappers) of five species of snappers (Lutjanidae), including Lutjanus analis, Lutjanus synagris, Lutjanus vivanus, Ocyurus chrysurus, and Romboplites saliens at five sites along the northeast (Riacho Doce, Maceió in Alagoas State, and Porto do Sauípe, Entre Rios at Bahia State) and the southeast (SE) Brazilian coast (Paraty and Rio de Janeiro cities at Rio de Janeiro State, and Bertioga, at São Paulo State.). We collected 288 snappers and interviewed 86 fishermen. The stomach contents of each fish were examined and macroscopic gonad analysis was performed. Snappers are very important for the fisheries of NE Brazil, and our results indicated that some populations, such as mutton snapper (L. analis) and lane snapper (L. synagris), are being caught when they are too young, at early juvenile stages. Local knowledge has been shown to be a powerful tool for determining appropriate policies regarding management of target species, and artisanal fishermen can be included in management processes. Other suggestions for managing the fisheries are discussed, including proposals that could provide motivation for artisanal fishermen to participate in programs to conserve resources, such as co-management approaches that utilize local knowledge, the establishment of fishing seasons, and compensation of fishermen, through 'payment for environmental services'. These suggestions may enhance the participation of local artisanal fishermen in moving to a more realistic and less top-down management approach of the fish population. PMID:21410969

  2. Ethnobiology of snappers (Lutjanidae): target species and suggestions for management.

    PubMed

    Begossi, Alpina; Salivonchyk, Svetlana V; Araujo, Luciana G; Andreoli, Tainá B; Clauzet, Mariana; Martinelli, Claudia M; Ferreira, Allan G I; Oliveira, Luiz E C; Silvano, Renato A M

    2011-03-16

    In this study, we sought to investigate the biology (diet and reproduction) and ethnobiology (fishers knowledge and fishing spots used to catch snappers) of five species of snappers (Lutjanidae), including Lutjanus analis, Lutjanus synagris, Lutjanus vivanus, Ocyurus chrysurus, and Romboplites saliens at five sites along the northeast (Riacho Doce, Maceió in Alagoas State, and Porto do Sauípe, Entre Rios at Bahia State) and the southeast (SE) Brazilian coast (Paraty and Rio de Janeiro cities at Rio de Janeiro State, and Bertioga, at São Paulo State.).We collected 288 snappers and interviewed 86 fishermen. The stomach contents of each fish were examined and macroscopic gonad analysis was performed. Snappers are very important for the fisheries of NE Brazil, and our results indicated that some populations, such as mutton snapper (L. analis) and lane snapper (L. synagris), are being caught when they are too young, at early juvenile stages.Local knowledge has been shown to be a powerful tool for determining appropriate policies regarding management of target species, and artisanal fishermen can be included in management processes. Other suggestions for managing the fisheries are discussed, including proposals that could provide motivation for artisanal fishermen to participate in programs to conserve resources, such as co-management approaches that utilize local knowledge, the establishment of fishing seasons, and compensation of fishermen, through 'payment for environmental services'. These suggestions may enhance the participation of local artisanal fishermen in moving to a more realistic and less top-down management approach of the fish population.

  3. Interactions between range-expanding tropical fishes and the northern Gulf of Mexico red snapper Lutjanus campechanus.

    PubMed

    Marshak, A R; Heck, K L

    2017-09-14

    Experimental investigation of the intensity of potential competitive interactions among increasingly abundant tropically-associated grey Lutjanus griseus and lane snapper Lutjanus synagris and resident northern Gulf of Mexico (nGOM) red snapper Lutjanus campechanus was undertaken in large outdoor mesocosms. In pair-wise interaction trials, compared with L. synagris, L. campechanus demonstrated significantly increased roving behaviour and predatory activity. While no significant difference in these activities was observed between L. campechanus and L. griseus, when all three snappers (Lutjanidae) were grouped together L. campechanus swimming activity significantly decreased in the presence of both tropically-associated species. Overall, L. campechanus were more active and aggressive predators and appear to be competitively resistant to L. griseus and L. synagris. As lower latitude species have continued to become increasingly prevalent in nGOM habitats and regional warming continues to affect resident reef-associated fishes, these findings contribute to the assessment of the effects of warming-related species shifts upon nGOM fishes and document current partial resilience of L. campechanus to climate-related expansions of tropical confamilials. © 2017 The Fisheries Society of the British Isles.

  4. Neolebouria blatta n. sp. (Digenea: Opecoelidae) from Pristipomoides argyrogrammicus (Valenciennes) and Etelis carbunculus Cuvier (Perciformes: Lutjanidae) off New Caledonia.

    PubMed

    Bray, Rodney A; Justine, Jean-Lou

    2009-11-01

    Neolebouria blatta n. sp. is described from Pristipomoides argyrogrammicus (Valenciennes) and Etelis carbunculus Cuvier in waters off New Caledonia. It differs from all other species of Neolebouria Gibson, 1976 but one, N. georgenascimentoi Bray, 2002, in the extension of the cirrus-sac to the ovary or nearly so. It differs from N. georgenascimentoi in its continuous, rather than interrupted, vitelline distribution. N. blatta belongs to a small group of similar Neolebouria species reported in deep-water lutjanids, which includes N. longisacculus (Yamaguti, 1970) n. comb., N. rooseveltiae (Yamaguti, 1970) n. comb. and N. ulaula (Yamaguti, 1970).

  5. Kudoa hypoepicardialis n. sp. (Myxozoa: Kudoidae) and associated lesions from the heart of seven perciform fishes in the northern Gulf of Mexico.

    PubMed

    Blaylock, Reginald B; Bullard, Stephen A; Whipps, Christopher M

    2004-06-01

    Kudoa hypoepicardialis n. sp. infects the space between the epicardium and the compact myocardium and, in intense infections, the pericardial chamber of man-of-war fish (Nomeus gronovii) (Nomeidae) (the type host), blue runner (Caranx crysos) (Carangidae), Warsaw grouper (Epinephelus nigritus) (Serranidae), Atlantic tripletail (Lobotes surinamensis) (Lobotidae), northern red snapper (Lutjanus campechanus) (Lutjanidae), black drum (Pogonias cromis) (Sciaenidae), and bluefish (Pomatomus saltatrix) (Pomatomidae) in the northern Gulf of Mexico. This is the first report of a Kudoa sp. from the heart of a fish in the Gulf of Mexico, and of these hosts, only the bluefish was previously identified as a host for a species of Kudoa. Spores of the new species varied slightly in size among these hosts but were regarded as conspecific based on their nearly identical (99.9%) small-subunit (SSU) ribosomal DNA (rDNA) sequence. The new species differs both from the 4 nominal species of Kudoa reported from fishes in the Gulf of Mexico and from K. pericardialis, an allopatric species that infects the pericardial cavity, by the combination of having a large spore, a small polar capsule, and a polar filament with a single coil. The new species is morphologically and genetically most similar to K. shiomitsui, an allopatric species that infects the heart and pericardial cavity, but is distinguished from it based on a 4.2% difference in the SSU rDNA sequence. Heart lesions primarily were restricted to the vicinity of plasmodia and included a layer of fibrinous inflammation characterized by lymphocytes, macrophages, and granulomas as well as epithelioid encapsulations around plasmodia. Heavily infected hosts had melanin-like deposits and adipose cells beneath the epicardium. and the epicardium was discontinuous and apparently breached by plasmodia in some regions. Cardiac muscle, gill, liver, spleen, intestine, and kidney were normal.

  6. High levels of genetic connectivity among populations of yellowtail snapper, Ocyurus chrysurus (Lutjanidae-Perciformes), in the western South Atlantic revealed through multilocus analysis.

    PubMed

    da Silva, Raimundo; Veneza, Ivana; Sampaio, Iracilda; Araripe, Juliana; Schneider, Horacio; Gomes, Grazielle

    2015-01-01

    In the present study, five loci (mitochondrial and nuclear) were sequenced to determine the genetic diversity, population structure, and demographic history of populations of the yellowtail snapper, Ocyurus chrysurus, found along the coast of the western South Atlantic. O. chrysurus is a lutjanid species that is commonly associated with coral reefs and exhibits an ample geographic distribution, and it can therefore be considered a good model for the investigation of phylogeographic patterns and genetic connectivity in marine environments. The results reflected a marked congruence between the mitochondrial and nuclear markers as well as intense gene flow among the analyzed populations, which represent a single genetic stock along the entire coast of Brazil between the states of Pará and Espírito Santo. Our data also showed high levels of genetic diversity in the species (mainly mtDNA), as well a major historic population expansion, which most likely coincided with the sea level oscillations at the end of the Pleistocene. In addition, this species is intensively exploited by commercial fisheries, and data on the genetic structure of its populations will be essential for the development of effective conservation and management plans.

  7. High Levels of Genetic Connectivity among Populations of Yellowtail Snapper, Ocyurus chrysurus (LutjanidaePerciformes), in the Western South Atlantic Revealed through Multilocus Analysis

    PubMed Central

    da Silva, Raimundo; Veneza, Ivana; Sampaio, Iracilda; Araripe, Juliana; Schneider, Horacio; Gomes, Grazielle

    2015-01-01

    In the present study, five loci (mitochondrial and nuclear) were sequenced to determine the genetic diversity, population structure, and demographic history of populations of the yellowtail snapper, Ocyurus chrysurus, found along the coast of the western South Atlantic. O. chrysurus is a lutjanid species that is commonly associated with coral reefs and exhibits an ample geographic distribution, and it can therefore be considered a good model for the investigation of phylogeographic patterns and genetic connectivity in marine environments. The results reflected a marked congruence between the mitochondrial and nuclear markers as well as intense gene flow among the analyzed populations, which represent a single genetic stock along the entire coast of Brazil between the states of Pará and Espírito Santo. Our data also showed high levels of genetic diversity in the species (mainly mtDNA), as well a major historic population expansion, which most likely coincided with the sea level oscillations at the end of the Pleistocene. In addition, this species is intensively exploited by commercial fisheries, and data on the genetic structure of its populations will be essential for the development of effective conservation and management plans. PMID:25769032

  8. Sperm cryopreservation of lane snapper Lutjanus synagris (Linnaeus, 1758).

    PubMed

    Sanches, E G; Oliveira, I R; Serralheiro, P C S; Cerqueira, V R

    2015-08-01

    This study aims developing and evaluate a protocol of semen cryopreservation of the lane snapper Lutjanus synagris. Firstly, sperm motility rate, motility time, density and spermatocrit were appraised to characterize the sperm quality of the lane snapper. The effect of three extenders with distinct ionic compositions and pH values combined with seven concentrations of cryoprotector dimethylsulfoxide (0; 2.5; 5.0; 7.5; 10.0; 12.5 e 15.0%), five cooling rates (110, 90, 60, 45 e 30°C -min), nine equilibration time (1; 2,5; 5; 10; 15; 20; 25; 30 e 60 minutes) e five dilutions ratio (1:1; 1:3; 1:6; 1:10 e 1:20) on the sperm motility rate and motility time were analyzed. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The higher sperm motility rate and motility time (P<0.05) was achieved by combining extender with pH 8.2 with 10% concentration of dimethylsulfoxide and cooling rate 60°C -min, 1 minute of equilibration time and 1:3 (v/v) dilution ratio. The use of cryopreserved sperm presented fertilization rates >60% validating the present protocol for lane snapper. The cryoconserved sperm of lane snapper is a viable alternative, being possible to maintain appropriate sperm viability.

  9. A mitogenomic perspective on the phylogenetic position of the Hapalogenys genus (Acanthopterygii: Perciformes) and the evolutionary origin of Perciformes.

    PubMed

    Wei, Tao; Sun, Yuena; Zhang, Bo; Wang, Rixin; Xu, Tianjun

    2014-01-01

    The Hapalogenys genus was the most controversial and problematic in phylogenetic position of Percoidei. In this study, we rechecked the taxonomic status of Hapalogenys in Percoidei using complete mitochondrial genome data. We purposefully added a new complete mitochondrial sequence from chosen species of Hapalogenys and conducted phylogenetic analyses using a large complete mitochondrial data set. The resultant tree topologies were congruent from partitioned Bayesian and Maximum-likelihood methods. The results indicated that Hapalogenys was distantly related to Haemulidae and could be removed from Haemulidae. The results supported the Hapalogeny was upgraded to a family rank titled Hapalogenyidae, and it should be recognized in a separate family of Hapalogenyidae. A relaxed molecular-clock Bayesian analysis indicated that the divergence times of Perciformes groups had a much older than the available old fossil records. The origin of the common ancestral lineage of Perciformes fish was estimated in the late Jurassic about 149 Myr ago.

  10. A Mitogenomic Perspective on the Phylogenetic Position of the Hapalogenys Genus (Acanthopterygii: Perciformes) and the Evolutionary Origin of Perciformes

    PubMed Central

    Wei, Tao; Sun, Yuena; Zhang, Bo; Wang, Rixin; Xu, Tianjun

    2014-01-01

    The Hapalogenys genus was the most controversial and problematic in phylogenetic position of Percoidei. In this study, we rechecked the taxonomic status of Hapalogenys in Percoidei using complete mitochondrial genome data. We purposefully added a new complete mitochondrial sequence from chosen species of Hapalogenys and conducted phylogenetic analyses using a large complete mitochondrial data set. The resultant tree topologies were congruent from partitioned Bayesian and Maximum-likelihood methods. The results indicated that Hapalogenys was distantly related to Haemulidae and could be removed from Haemulidae. The results supported the Hapalogeny was upgraded to a family rank titled Hapalogenyidae, and it should be recognized in a separate family of Hapalogenyidae. A relaxed molecular-clock Bayesian analysis indicated that the divergence times of Perciformes groups had a much older than the available old fossil records. The origin of the common ancestral lineage of Perciformes fish was estimated in the late Jurassic about 149 Myr ago. PMID:25077480

  11. Iron in the enameloid of perciform fish.

    PubMed

    Suga, S; Taki, Y; Ogawa, M

    1992-06-01

    It is known that a high concentration of iron is deposited in the enameloid of some teleostean fish. Previously, Suga et al. (1989) pointed out that the iron concentration in the enameloid is related to the phylogeny of fish rather than to the feeding habits, according to the results of quantitative iron analyses on the teeth of marine teleost fish of the Tetraodontiformes. In the present study, in order for the previous idea to be verified, quantitative iron analysis was made with an electron microprobe on the enameloid of fish belonging to the Perciformes, which is the largest group of teleostean fish in the world and consists of both marine and freshwater species. The enameloid of all the fish examined (57 species) contained high iron concentrations ranging from 0.2% to 10.2% at the surface or middle layer, whereas that of an advanced suborder, Tetraodontoidei, of the Tetraodontiformes was very low in iron, at a level which could not be discriminated from the background value of the emission intensity. The distribution pattern of iron in the enameloid was classified into at least two types, namely, type A, in which a high iron concentration was observed mainly in the surface layer, and type B, in which iron was deposited throughout the entire layer, although there were differences in concentration. There were some differences in the concentration and distribution of iron in the enameloid for the families; for example, those of the Scaridae had a type A distribution, with about 0.2% iron only at the surface layer, whereas those of the Cichlidae, Centrarchidae, and Acanthuridae, which showed a type B distribution, contained iron ranging from 2.9% to 10.5% at the surface or middle layer of enameloid. Such differences seemed to be associated with the difference in timing of the commencement of the iron deposition into the developing enameloid, which is probably related to the phylogeny of fish. There was no evidence to support the idea that the iron concentration in

  12. Microbiota of wild-caught Red Snapper Lutjanus campechanus.

    PubMed

    Tarnecki, Andrea M; Patterson, William F; Arias, Covadonga R

    2016-10-21

    The microbiota plays an essential role in host health, particularly through competition with opportunistic pathogens. Changes in total bacterial load and microbiota structure can indicate early stages of disease, and information on the composition of bacterial communities is essential to understanding fish health. Although Red Snapper (Lutjanus campechanus) is an economically important species in recreational fisheries and a primary aquaculture candidate, no information is available on the microbial communities of this species. The aim of this study was to survey the microbiota of apparently healthy, wild-caught Red Snapper from the Gulf of Mexico. Sampled Red Snapper showed no physical signs of disease. Tissues that are either primary entry routes for pathogens (feces, gill) or essential to disease diagnosis (blood) were sampled. Bacteria were enumerated using culture-based techniques and characterized by pyrosequencing. Aerobic counts of feces and gill samples were 10(7) and 10(4) CFU g(-1), respectively. All individuals had positive blood cultures with counts up to 23 CFU g(-1). Gammaproteobacteria dominated the microbiota of all sample types, including the genera Pseudoalteromonas and Photobacterium in feces and Pseudomonas in blood and gill. Gill samples were also dominated by Vibrio while blood samples had high abundances of Nevskia. High variability in microbiota composition was observed between individuals, with percent differences in community composition ranging from 6 to 76 % in feces, 10 to 58 % in gill, and 52 to 64 % in blood. This study provides the first characterization of the microbiota of the economically significant Red Snapper via pyrosequencing. Its role in fish health highlights the importance of understanding microbiota composition for future work on disease prevention using microbial manipulation.

  13. Carbon isotopes in otolith amino acids identify residency of juvenile snapper (Family: Lutjanidae) in coastal nurseries

    NASA Astrophysics Data System (ADS)

    McMahon, K. W.; Berumen, M. L.; Mateo, I.; Elsdon, T. S.; Thorrold, S. R.

    2011-12-01

    This study explored the potential for otolith geochemistry in snapper (Family: Lutjanidae) to identify residency in juvenile nursery habitats with distinctive carbon isotope values. Conventional bulk otolith and muscle stable isotope analyses (SIA) and essential amino acid (AA) SIA were conducted on snapper collected from seagrass beds, mangroves, and coral reefs in the Red Sea, Caribbean Sea, and Pacific coast of Panama. While bulk stable isotope values in otoliths showed regional differences, they failed to distinguish nursery residence on local scales. Essential AA δ13C values in otoliths, on the other hand, varied as a function of habitat type and provided a better tracer of residence in different juvenile nursery habitats than conventional bulk otolith SIA alone. A strong linear relationship was found between paired otolith and muscle essential AA δ13C values regardless of species, geographic region, or habitat type, indicating that otolith AAs recorded the same dietary information as muscle AAs. Juvenile snapper in the Red Sea sheltered in mangroves but fed in seagrass beds, while snapper from the Caribbean Sea and Pacific coast of Panama showed greater reliance on mangrove-derived carbon. Furthermore, compound-specific SIA revealed that microbially recycled detrital carbon, not water-column-based new phytoplankton carbon, was the primary carbon source supporting snapper production on coastal reefs of the Red Sea. This study presented robust tracers of juvenile nursery residence that will be crucial for reconstructing ontogenetic migration patterns of fishes among coastal wetlands and coral reefs. This information is key to determining the importance of nursery habitats to coral reef fish populations and will provide valuable scientific support for the design of networked marine-protected areas.

  14. Growth stanzas in an Epinephelidae-Lutjanidae complex: considerations to length-weight relationships.

    PubMed

    Renán, Ximena; Trejo-Martínez, Jorge; Caballero-Arango, Doralice; Brulé, Thierry

    2015-03-01

    Growth stanzas or abrupt changes in growth rates are present throughout the life span of fish. Identifying growth stanzas will help to adequately described growth taking into account that fishes are indeterminate growers. In this study, we used length-weight (L-W) relationships to analyze the growth stanzas in the Grouper-Snapper complex of Southern Gulf of Mexico. For this, the type of sexuality, sex and different sexual maturity phase were considered in the analyses of three species of gonochoric Snappers (Lutjanidae) and six species of protogynous hermaphrodite Groupers (Epinephelidae). Welch ANOVA tests were carried out to deter- mine the existence of differences in length and weight between juveniles and adults per sex. According to the observed differences, L-W relationship parameters (a and b), standard error (SE b) and coefficients of determination (R2) were calculated for all species separately by sex and sexual maturity phase. Snappers' juvenile-females b-value ranged from 2.44-2.77, juvenile-males from 2.16-2.94, adult-females from 2.63-2.80 and adult-males from 2.63-2.98. Groupers' b-value ranged for juvenile-females 2.66-3.20, adult-females from 2.73-3.31 and for adult-males 2.93-3.29. For each relationship b-value was 1-tested (-Student) to explore differences from the allometric coefficient (b=3), which indicated changes in body form. Hypothesis test, for regression slopes (b) between Snappers' juvenile-females vs. adult-females and juvenile-males vs. adult-males and Groupers' juvenile-females vs. adult-females and adult-females vs. adult-males, indicated different growth stanzas related to gonadal development for Snappers, and to gonadal development and sex change in Groupers. The identification of growth stanzas is crucial to avoid an overestimation or misleading growth rate which is used in fisheries management to establish some target reference points, such as maximum sustainable yield or yield-per-recruit.

  15. Chromosomal complements of some Atlantic Blennioidei and Gobioidei species (Perciformes)

    PubMed Central

    Galvão, Tatiana Barbosa; Bertollo, Luiz Antonio Carlos; Molina, Wagner Franco

    2011-01-01

    Abstract A remarkable degree of chromosomal conservatism (2n=48, FN=48) has been identified in several families of Perciformes. However, some families exhibit greater karyotypic diversity, although there is still scant information on the Atlantic species. In addition to a review of karyotypic data available for representatives of the suborders Blennioidei and Gobioidei, we have performed chromosomal analyses on Atlantic species of the families Blenniidae, Ophioblennius trinitatis Miranda-Ribeiro, 1919 (2n=46; FN=64) and Scartella cristata (Linnaeus, 1758)(2n=48; FN=50), Labrisomidae, Labrisomus nuchipinnis (Quoy & Gaimard, 1824)(2n=48; FN=50) and Gobiidae, Bathygobius soporator (Valenciennes, 1837)(2n=48; FN=56). Besides variations in chromosome number and karyotype formulas, Ag-NOR sites, albeit unique, were located in different positions and/or chromosome pairs for the species analyzed. On the other hand, the heterochromatic pattern was more conservative, distributed predominantly in the centromeric/pericentromeric regions of the four species. Data already available for Gobiidae, Blenniidae and Labrisomidae show greater intra- and interspecific karyotypic diversification when compared to other groups of Perciformes, where higher uniformity is found for various chromosome characteristics. Evolutionary dynamism displayed by these two families is likely associated with population fractionation resulting from unique biological characteristics, such as lower mobility and/or specific environmental requirements. PMID:24260634

  16. Two gonad-infecting species of Philometra (Nematoda: Philometridae) from marine fishes off the northern coast of Australia.

    PubMed

    Moravec, František; Barton, Diane P

    2015-01-01

    Two different gonad-infecting species of Philometra Costa, 1845 were collected from the ovary of marine perciform fishes, the blackspotted croaker Protonibea diacanthus (Sciaenidae) and the John's snapper Lutjanus johnii (Lutjanidae), from off the northern coast of Australia. Nematodes (males and females) from P. diacanthus represent a new taxon, Philometra protonibeae n. sp., which is mainly characterized by the body length of the males (3.37-3. 90 mm), broad, equally long spicules (length 126-141 μm) and the shape and structure of the gubernaculum with a dorsally lamellate distal tip. The nematodes (only females) from L. johnii may represent an undescribed species, but, because of the absence of conspecific males, they could not be specifically identified. Philometra protonibeae is the fifth nominal gonad-infecting species of this genus recorded from marine fishes in Australian waters and the seventh species of these parasites described from fishes of the family Sciaenidae.

  17. The complete mitochondrial genome sequence of Pampus chinensis (Perciformes: Stromateidae).

    PubMed

    Sun, Dandan; Cheng, Qiqun; Qiao, Huiying; Zhang, Heng; Chen, Ying

    2016-01-01

    In this study, the complete mitochondrial genome of Pampus chinensis (Perciformes: Stromateidae) was determined. The mitogenome is 16,535 bp in length, which contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 2 non-coding regions: origin of light-strand replication (OL) and control region (D-loop). The overall mtDNA nucleotide base composition of P. chinensis is A 29.72%, C 28.10%, G 15.34%, and T 26.84%, with an A + T content of 56.56%. Except for ND6 gene and eight tRNA genes, all other mitochondrial genes were encoded on the heavy strand. The mitochondrial genome of P. chinensis may be helpful to the studies on stock evaluation and conservation genetics of P. chinensis resource, as well as molecular phylogeny of Stromateidae.

  18. [Ultrastructure of granulocytes of bony fishes (orders Salmoniformes, Cypriniformes, Perciformes)].

    PubMed

    Flerova, E A; Balabanova, L V

    2013-01-01

    Analysis of data on utrastructure of granulocytes of freshwater and marine bony fish of orders Salmoniformes, Cypriniformes, and Perciformes showed that in all studied species there were revealed two types of granulocytes - neutrophils and eosinophils. The exception was the bluefish Pomatomus saltatrix L. whose pronephros hemopoietic tissue was found to contain one type of the granulocytic line - neutrophils. The identification parameters of granular leukocytes are specific granules filling the cytoplasm. The main form of specific granules in neutrophils of bony fish of various phylogenetic groups is an elongated granule with different distribution of fibrils or a granule that has crystalloid formed from fibrils. The main form of eosinophil granules - large, electron-dense, homogenous.

  19. The complete mitochondrial genome sequence of Pampus argenteus (Perciformes: Stromateidae).

    PubMed

    Sun, Dandan; Cheng, Qiqun; Qiao, Huiying; Chen, Ying

    2016-01-01

    In this study, we sequenced and annotated the complete mitochondrial genome of Pampus argenteus (Perciformes: Stromateidae). The mitogenome is 17,098 bp in length, which contains 13 protein-coding genes, 2 rRNA genes, 23 tRNA genes and 2 non-coding regions: origin of light-strand replication (OL) and control region (D-loop). The overall nucleotide base composition of P. argenteus mtDNA is A 30.35%, C 25.55%, G 15.28% and T 28.82%, with an A + T content of 59.17%. Except for ND6 gene and eight tRNA genes, all other mitochondrial genes were encoded on the heavy strand. The mitochondrial genome of P. argenteus may be helpful to the studies on conservation genetics and stock evaluation of P. argenteus resource, as well as molecular phylogeny and species identification of Stromateidae.

  20. Complete mitochondrial genome of the mudskipper Boleophthalmus boddarti (Perciformes, Gobiidae).

    PubMed

    Zhang, Yu Ting; Ghaffar, Mazlan Abd; Li, Zhe; Chen, Wei; Chen, Shi Xi; Hong, Wan Shu

    2016-01-01

    The Boddart's goggle-eyed mudskipper, Boleophthalmus boddarti (Perciformes, Gobiidae) is an amphibious fish, inhabiting brackish waters of estuaries and builds burrows in soft mud along the intertidal zone. In this paper, the complete mitochondrial genome sequence of B. boddarti was firstly determined. The circle genome (16,727 bp) comprises 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region. The overall base composition of B. boddarti is 29.1% for C, 28.9% for A, 25.9% for T, and 16.0% for G, with a slight A + T bias of 54.8%. The termination-associated sequence, conserved sequence block domains, and a 131-bp tandem repeat were found in the control region. It has the typical vertebrate mitochondrial gene arrangement.

  1. Bothriocephalidean tapeworms (Cestoda) from the blackfish, Centrolophus niger (Perciformes: Centrolophidae).

    PubMed

    Kuchta, Roman; Scholz, Tomás; Brabec, Jan; Jirsová, Dagmar; Gustinelli, Andrea

    2008-06-01

    Bothriocephalidean tapeworms parasitic in the blackfish, Centrolophus niger (Gmelin) (Perciformes: Centrolophidae), are redescribed on the basis of the evaluation of freshly collected specimens and museum material. This evaluation enabled us to supplement species diagnoses by new morphological characters of potential use for phylogenetic analyses, including the data from scanning electron microscopical observations, and to provide a key to identification of the following four species occurring in this fish: Amphicotyle heteropleura (Diesing, 1850); Milanella familiaris Kuchta et Scholz, 2008 (both Triaenophoridae); Bothriocotyle solinosomum Ariola, 1900; and Echinophallus wageneri (Monticelli, 1890) (both Echinophallidae). Large spiniform microtriches were observed on the surface of the posterodorsal margin of segments of B. solinosomum, E. wageneri and M. familiaris. The invalidity of Atelemerus Guiart, 1935, first proposed by Bray et al. (1994), is supported by the present data and its type species, A. acanthodes Guiart, 1935, is newly synonymised with E. wageneri.

  2. Use of riverine through reef habitat systems by dog snapper ( Lutjanus jocu ) in eastern Brazil

    NASA Astrophysics Data System (ADS)

    Moura, Rodrigo L.; Francini-Filho, Ronaldo B.; Chaves, Eduardo M.; Minte-Vera, Carolina V.; Lindeman, Kenyon C.

    2011-11-01

    The early life history of Western Atlantic snappers from the Southern hemisphere is largely unknown. Habitat use of different life stages (i.e. size categories) of the dog snapper ( Lutjanus jocu) was examined across the largest South Atlantic reef-estuarine complex (Abrolhos Shelf, Brazil, 16-19° S). Visual surveys were conducted in different habitats across the shelf (estuary, inner-shelf reefs and mid-shelf reefs). Lutjanus jocu showed higher densities on inner-shelf habitats, with a clear increase in fish size across the shelf. Individuals <7 cm were associated with both the estuary (mangrove and rocky habitats) and inner-shelf reefs (particularly shallow fore-reefs and tide pools). Individuals ranging 10-30 cm were broadly distributed, but consistently more abundant on inner-shelf reefs. Individuals between 30 and 40 cm were more common on mid-shelf reefs, while individuals >40 cm were recorded only on mid-shelf reefs. Literature data indicate that individuals ranging 70-80 cm are common on deep offshore reefs. This pattern suggests that the dog snapper performs ontogenetic cross-shelf migrations. Protecting portions of the different habitats used by the dog snapper during its post-settlement life cycle is highlighted as an important conservation and management measure.

  3. Robertsonian rearrangements and pericentric inversions in Scaridae fish (Perciformes).

    PubMed

    Sena, D C S; Molina, W F

    2007-09-30

    The parrotfishes (family Scaridae) are comprised of the subfamilies Sparisomatinae and Scarinae. They are important agents of marine bioerosion, which rework the substrate with their beaklike jaws. Despite their importance, there are no published cytogenetic data on this group. We made cytogenetic analyses of Sparisoma axillare (Sparisomatinae) and Scarus trispinosus [corrected] (Scarinae) from the Brazilian coast. Differentiation in the diploid number in S. axillare compared to the basal karyotype of the Perciformes apparently occurred due to a Robertsonian fusion, combined with pericentric inversions. S. trispinosus [corrected] presented a conserved diploid number, but showed considerable structural karyotypic changes, resulting mainly from pericentric inversions. The Ag-NOR sites were unique and located on the short arm of the 1st subtelocentric pair in both species (possibly homeologous), corresponding to the 11th pair in S. axillare and the 9th pair in S. trispinosus [corrected] The constitutive heterochromatin is reduced in these species and is distributed in centromeric and pericentromeric regions in most of the chromosomes. The low fundamental number compared to the Scarus genus suggests a more basal condition for Sparisoma. The chromosome formula in S. trispinosus [corrected] was more diversified, deriving from large-scale pericentric inversions. Karyotypic evolution patterns observed for these representatives of the Sparisomatinae and Scarinae subfamilies, added to new data from a larger number of species, would allow us to determine if there is a tendency among the Sparisomatinae for centric fusion events.

  4. Cytogenetic studies in Brazilian marine Sciaenidae and Sparidae fishes (Perciformes).

    PubMed

    Accioly, I V; Molina, W F

    2008-04-22

    Fishes from the families Sciaenidae and Sparidae, the former comprising coastal species associated with shallow waters on the continental shelf and the latter composed of typically marine species, are of significant economic value. Karyotypic data are available for about 20% of the total number of species in these groups. In the present study, cytogenetic analyses were carried out in three Sciaenidae species, Menticirrhus americanus, Ophioscion punctatissimus and Pareques acuminatus, as well as in the sparid fish, Archosargus probatocephalus, using conventional staining (Giemsa) and Ag-nucleolar organizer regions (NORs) and C-banding techniques. The diploid values (2n) and number of chromosome arms were equal to 48 in all species analyzed. NORs were located at pericentromeric positions, equivalent to large heterochromatic blocks, in M. americanus (1st pair), O. punctatissimus (10th pair), P. acuminatus (2nd pair), and A. probatocephalus (3rd pair). Heterochromatin was detected at the centromeric position in most chromosome pairs, being more conspicuous among Scianidae members. The remarkable karyotypic conservativeness detected in these species is similar to that observed in other perciform groups previously studied, regarding both the number of acrocentric chromosomes and NOR location. However, unusual events of heterochromatinization seem to have taken place along the karyotypic evolution of members of the family Sciaenidae. For the family Sparidae, distinct cytotypes between samples of Northeast Brazil and those previously analyzed on the southeastern coast were identified, suggesting that putative biogeographic barriers could be present throughout both regions on South Atlantic coast.

  5. [Bacteriological load of the fishes Cynoscion squamipinnis and Lutjanus gutattus in the marketing chain, Costa Rica].

    PubMed

    Marín, Carolina; Fonseca, Cristian; Arias, Sidey; Villegas, Irene; García, Andrea; Ishihara, Hikaru

    2009-01-01

    Bacteriological load of the fishes Cynoscion squamipinnis and Lutjanus gutattus in the marketing chain, Costa Rica. To determine the bacteriological quality of fishery products in the different stages from commercialization, monthly samples were taken during March 2004 and February 2006 from a Costa Rica marketing chain. Microbiological analyses were made to determine total coliforms (CT), faecal coliforms (CF), Escherichia coli (EC), aerobic total count (RTA), Salmonella sp., Staphylococcus aureus, Vibrio cholerae and Vibrio parahaemolyticus. Three body zones were analyzed: skin, belly and muscle. There were differences in the amount of CT between parts: skin had the highest counts, 11% of samples were identified as E. coli. and 2.5% of total counts were higher than the legal limit. Only 1.3% of the samples were S. aureus-positive. Salmonella sp., V. cholerae and V. parahaemolyticus were absent.

  6. Some camallanid nematodes from marine perciform fishes off New Caledonia.

    PubMed

    Moravec, Frantisek; Justine, Jean-Lou; Rigby, Mark C

    2006-09-01

    Two new, one known and three unidentified species of the nematode family Camallanidae are reported and described from the intestines of marine perciform fishes off the southwestern coast of New Caledonia, South Pacific: Camallanus carangis Olsen, 1952 from the forked-tailed threadfin bream Nemipterus furcosus (Nemipteridae), the yellowstriped goatfish Upeneus vittatus and the whitesaddle goatfish Parupeneus ciliatus (both Mullidae) (new host records); Procamallanus (Spirocamallanus) variolae sp. n. from the white-edged lyretail Variola albimarginata (type host) and the blacktip grouper Epinephelus fasciatus (both Serranidae); Procamallanus (Spirocamallanus) longus sp. n. from the twotone tang Zebrasoma scopas (Acanthuridae); Procamallanus (Spirocamallanus) sp. 1 (female tail with 2 terminal spikes on a digit-like projection) from the speckled sand-perch Parapercis hexophtalma (Pinguipedidae); Procamallanus (Spirocamallanus) sp. 2 (female tail with 1 spike on a digit-like projection) from the drab emperor Lethrinus ravus (Lethrinidae) and Procamallanus (Spirocamallanus) sp. 3 (female tail with a smooth digit-like protrusion) from the two-lined monocle bream Scolopsis bilineata (Nemipteridae). Camallanus paracarangis Velasquez, 1980 is synonymized with C. carangis. Several additional species of Camallanus from marine fish of the Indo-Pacific region may be synonymous with C. carangis as it has a poorly sclerotized left spicule and 3 small caudal projections on the tail of young (i.e., non-gravid) females. The fourth-stage larva of C. carangis is described for the first time. P. (S.) variolae differs from most similar species of this region mainly in the position (i.e., at level or posterior to the nerve ring) and shape of deirids. P. (S.) longus differs from the similar P. (S.) chaimha mainly in a different arrangement of postanal papillae, shape of the female tail, much longer right spicule (429 microm) and longer body of gravid females (38-55 mm). All Camallanus and

  7. Species delineation in Pampus (Perciformes) and the phylogenetic status of the Stromateoidei based on mitogenomics.

    PubMed

    Cui, Zhaoxia; Liu, Yuan; Li, Chi Pang; Chu, Ka Hou

    2011-02-01

    Recent studies on the mitochondrial genome have suggested that the duplications of tRNA and tandem repeats in the control region are important changes that are related to species diversity. This paper reports the study of mitogenomes from five Pampus (Perciformes, Stromateidae) species with very similar morphology. A duplicated tRNA(Met) gene in the tRNA-IQM region is present in Pampus sp. and P. punctatissimus. In the conserved sequence blocks of the control region, a duplicated CSB3 and promoter are found in Pampus sp. but are absent in P. minor. Moreover, a duplicated TAS is found in P. punctatissimus and P. chinensis. Based on the complete mitogenome sequence of Pampus sp., the first sequence reported from Stromateidae and the longest (17,694 bp) among the Perciformes mitogenomes, we conducted phylogenetic analysis to show that Stromateoidei and Scombroidei are more closely related to each other than to other Perciformes suborders. However, we reject the reciprocal monophyly of these two suborders.

  8. Diet variation of a generalist fish predator, grey snapper Lutjanus griseus, across an estuarine gradient: trade-offs of quantity for quality?

    PubMed

    Yeager, L A; Layman, C A; Hammerschlag-Peyer, C M

    2014-08-01

    This study examined diet, prey quality and growth for a generalist fish predator, grey snapper Lutjanus griseus, at five sites across an estuarine gradient in the Loxahatchee River estuary, Florida, U.S.A. Lutjanus griseus diets shifted from dominance by low quality, intertidal crabs upstream to an increased reliance on higher quality shrimp, fishes and benthic crabs downstream. Frequency of L. griseus with empty stomachs was higher at downstream sites. Lutjanus griseus growth rates did not vary among sites. Results indicate that L. griseus may be able to compensate for lower quality prey upstream by consuming more, and thus individuals are able to maintain similar levels of energy balance and growth rates across the estuarine gradient. Elucidating mechanisms, such as compensatory feeding, that enable generalist species to remain successful across habitat conditions are critical to understanding their organismal ecology and may facilitate predictions about the response of generalists to landscape alteration.

  9. Characterization of chitin and chitosan synthesized from red snapper (Lutjanus sp.) scale's waste

    NASA Astrophysics Data System (ADS)

    Takarina, N. D.; Fanani, A. A.

    2017-07-01

    Chitin and chitosan are natural biopolymer which are useful for industrial, medical and environmental field. Study about using fish scale sources especially saltwater fish is still limited. Red snapper (Lutjanus sp) is common tropical saltwater fish that known as important source of marine products, particularly in Indonesia. Correspondingly, the consumption of this species has generated significant amount of discarded scale wastes recently and hence can cause adverse impact on the environment. Utilizing the fish scale as alternative sources of chitin and chitosan can be one solution dealing with environmental problem. Therefore, this research aimed to characterize the chitin and chitosan derived from the red snapper scale wastes. Chitin were extracted by deproteination and demineralization while chitosan using deacetylation. Morphology of the chitin and chitosan were analyzed using electron dispersal spectroscopy (EDS) and scanning electron microscope (SEM), while degree of deacetylation using fourier transform infrared spectroscopy (FTIR). Proximate analysis showed that content of moisture, ash, and nitrogen in chitin were 3.20 %, 2.40 %, 0.04 %, respectively while in chitosan were 6.14 %, 1.18 %, 0.03 % respectively. Furthermore, amount of C, O, Na, Al, P and Ca elements were obtained from chitin and chitosan samples by energy dispersed spectroscopy respectively. The degree of deacetylation for both chitin and chitosan showed high value more than 75 %. Hence, by considering the chemical properties of red snapper scales, it confirms that this species is a promising alternative source for both chitin and chitosan.

  10. Salinity selection and preference of the grey snapper Lutjanus griseus: field and laboratory observations.

    PubMed

    Serrano, X; Grosell, M; Serafy, J E

    2010-05-01

    Field observations were supplemented with laboratory experiments to reveal patterns of salinity selection and preference for grey snapper Lutjanus griseus (c. 21 cm total length, L(T)), an ecologically and economically important species in the south-eastern U.S.A. Fish abundance data were examined from a long-term field survey conducted in the mangrove habitats of Biscayne Bay, Florida, where salinities ranged from <1 to 40. First, regression analyses indicated significant, positive linear relationships with salinity for both L. griseus frequency of occurrence and concentration (density when present). These patterns are inconsistent with physiological expectations of minimizing energetic osmoregulatory costs. Next, the salinity preference and swimming activity of 11 L. griseus (ranging from 18 to 23 cm L(T)) were investigated using a newly developed electronic shuttlebox system. In the laboratory, fish preferred intermediate salinities in the range of 9-23. Swimming activity (measured in terms of spontaneous swimming speed) followed a parabolic relationship with salinity, with reduced activity at salinity extremes perhaps reflecting compensation for higher osmoregulatory costs. It is suspected that the basis of the discrepancy between laboratory and field observations for size classes at or near maturity ultimately relates to the reproductive imperative to move towards offshore (high-salinity) coral-reef habitats, a necessity that probably overrides the strategy of minimizing osmoregulatory energetic costs.

  11. Optimisation of Deacetylation Process for Chitosan Production from Red Snapper (Lutjanus sp.) Scale Wastes

    NASA Astrophysics Data System (ADS)

    Takarina, N. D.; Indah, A. B.; Nasrul, A. A.; Nurmarina, A.; Saefumillah, A.; Fanani, A. A.; Loka, K. D. P.

    2017-02-01

    Red snapper (Lutjanus sp.) is common tropical fish that known as important source of marine product in particular Indonesia. This research aimed to optimise the chitosan synthesis from the red snapper scale waste through deacetylation process. Method in this research was divided into three stages which were deproteination, demineralization, and deacetylation. Deproteination stage was done with solution containing 4.2% w/v NaOH and heated at 60° C for 5 hours and followed by the demineralization stage with solution containing 52% v/v 2 N HCl at room temperature for 6 hours. The comparison between fish scales and solutions was 1: 6. After that, process continued with the deacetylation. Several treatment during the deacetylation process were taken into consideration to determine the effective concentration for yielding optimum chitosan output. Chitosan produced were having moisture content of 2.88%, ash content of 1.10%, and nitrogen content of 0.0136%. Optimal Degree of Deacetylation (DDA) was up to 90.83% that obtained by heating treatment at a temperature of 110° C with solution containing 80% NaOH for 4 hours, and comparison between chitin : solution was 1 : 3. This result indicated that chitosan extracted from red snapper scale is very potential and can be applied to industry.

  12. Is there a cryptic species of the golden snapper (Lutjanus johnii)?

    PubMed

    Adibah, A B; Darlina, M N

    2014-10-07

    For centuries, morphology-based fish identification has been applied without molecular evaluation. Many studies showed that specimens with a similar morphology are frequently found to be quite genetically distinct. One of the fish species that still remains taxonomically problematic is a commercial snapper species, Lutjanus johnii. Because of morphological ambiguities among local fish taxonomists in Malaysia, we examined the ability of the cytochrome oxidase I (COI) gene to genetically examine the taxonomic status of L. johnii. A 626-base pair COI region was successfully amplified and aligned with conspecific sequences that were retrieved from GenBank. The phylogenetic tree obtained showed two major clusters; the first cluster consists of L. johnii from Straits of Malacca, Thailand, Australia, and China while the second cluster comprises L. johnii from China and India. The latter group showed sequence divergence greater than 3.5%. After observing this, we suspected that there might be a cryptic species between the South China Sea and Indian Ocean. This is the first molecular report concerning the commercial species of snapper, L. johnii, in Malaysia, which had only gained provisional recognition from morphological examination.

  13. Cucullanus pargi sp. n. (Nematoda: Cucullanidae) from the grey snapper Lutjanus griseus off the southern coast of Quintana Roo, Mexico.

    PubMed

    González-Solís, David; Tuz-Paredes, Vielka M; Quintal-Loria, Miguel A

    2007-09-01

    A new nematode species, Cucullanus pargi sp. n., is described from the intestine and pyloric caeca of the grey snapper, Lutjanus griseus (Linnaeus), off the southern Quintana Roo coast, Mexico. This species shows similar morphological features as cucullanids occurring in marine and brackish-water fishes; however, it differs from all other species in the length of spicules, arrangement and number of caudal papillae, position of the excretory pore and deirids. Cucullanus pargi is the third species of this genus described from fishes in Mexico and the second one from Mexican marine fishes.

  14. Diet shifts of Caribbean grunts (Haemulidae) and snappers (Lutjanidae) and the relation with nursery-to-coral reef migrations

    NASA Astrophysics Data System (ADS)

    Cocheret de la Morinière, E.; Pollux, B. J. A.; Nagelkerken, I.; van der Velde, G.

    2003-08-01

    The spatial size distribution of grunts and snappers have previously indicated the separation of juveniles in nursery habitats from the adults on the coral reef. This implies life cycle migrations from nursery habitats (such as seagrass beds and mangroves) to the coral reef. If diet shifts are related to such migrations, then the diets of these fish must change before or around the fish size at which such migrations take place. A wide size range of juveniles of two grunt species ( Haemulon sciurus and Haemulon flavolineatum) and of two snapper species ( Lutjanus apodus and Ocyurus chrysurus) were caught in seagrass beds and mangroves, and their gut contents identified and quantified. Regression analysis between fish size and dietary importance of small crustaceans showed a negative relationship in all four species. Positive relations were found for H. sciurus, L. apodus and O. chrysurus between fish length and the dietary importance of decapods, and for L. apodusand O. chrysurus between fish length and prey fish importance. Critical changes in the fish diets with fish size were examined by application of a Canonical Correspondence Analysis (CCA). The CCA yielded three clusters of size-classes of fishes with similar diets, and application of a Mantel test showed that each of these clusters had significantly different diets, and that each cluster diet was significantly specialised. The size at which a fish species 'switched' from one cluster to another was compared with size-at-maturity data and with the typical size at which these species migrate from the nursery habitats to the coral reef. H. sciurus and H. flavolineatum may be prompted to migrate from the nursery habitats to coral reef habitats because of dietary changes, or because of the development of the gonads. For L. apodus and O. chrysurus, a dietary changeover forms a more likely explanation for nursery-to-reef migrations than does sexual maturation because these species reach maturity at sizes much larger

  15. Characterization of 32 microsatellite loci for the Pacific red snapper, Lutjanus peru, through next generation sequencing.

    PubMed

    Paz-García, David A; Munguía-Vega, Adrián; Plomozo-Lugo, Tomas; Weaver, Amy Hudson

    2017-04-01

    We developed a set of hypervariable microsatellite markers for the Pacific red snapper (Lutjanus peru), an economically important marine fish for small-scale fisheries in the west coast of Mexico. We performed shotgun genome sequencing with the 454 XL titanium chemistry and used bioinformatic tools to search for perfect microsatellite loci. We selected 66 primer pairs that were synthesized and genotyped in an ABI PRISM 3730XL DNA sequencer in 32 individuals from the Gulf of California. We estimated levels of genetic diversity, deviations from linkage and Hardy-Weinberg equilibrium, estimated the frequency of null alleles and the probability of individual identity for the new markers. We reanalyzed 16 loci in 16 individuals to estimate genotyping error rates. Eighteen loci failed to amplify, 16 loci were discarded due to unspecific amplifications and 32 loci (14 tetranucleotide and 18 dinucleotide) were successfully scored. The average number of alleles per locus was 21 (±6.87, SD) and ranged from 8 to 34. The average observed and expected heterozygosities were 0.787 (±0.144 SD, range 0.250-0.935) and 0.909 (±0.122 SD, range 0.381-0.965), respectively. No significant linkage was detected. Eight loci showed deviations from Hardy-Weinberg equilibrium, and from these, four loci showed moderate null allele frequencies (0.104-0.220). The probability of individual identity for the new loci was 1.46(-62). Genotyping error rates averaged 9.58%. The new markers will be useful to investigate patterns of larval dispersal, metapopulation dynamics, fine-scale genetic structure and diversity aimed to inform the implementation of spatially explicit fisheries management strategies in the Gulf of California.

  16. Complete mitochondrial genome of the spotted scat Scatophagus argus (Perciformes: Scatophagidae).

    PubMed

    Liu, Zhi Zhi; Zhang, Tian Rui; Su, Li Wei; Zhang, Jun Bin; Yang, Jin Quan

    2014-12-01

    The spotted scat, Scatophagus argus (Perciformes, Scatophagidae), is a recreational and commercial fish in China. In this paper, the complete mitochondrial genome of S. argus was firstly determined. It is 16,783 bp in length and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region. Except for the eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. The mitochondrial DNA information would be useful in species identification and natural resources conservation.

  17. Complete mitochondrial genome of the Easten hulafish, Trachinops taeniatus (Perciformes: Plesiopidae).

    PubMed

    Xia, Rong; Fu, Cuizhang

    2015-01-01

    The first complete mitochondrial genome (mitogenome) of Plesiopidae (Teleostei: Perciformes) was determined using the Eastern hulafish Trachinops taeniatus as a representative species of the family. The mitogenome was 16,821 bp in length, including 13 typical vertebrate protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 1 control region. The gene arrangement was similar to other fishes except for the reversal position of tRNA(Ala) and tRNA(Asn). The overall base composition was 27.4% for A, 27.5% for C, 27.4% for T and 17.7% for G. This mitogenome should contribute to resolving phylogenetic position of Plesiopidae.

  18. A new species of diplectanid (Monogenoidea) from Paranthias colonus (Perciformes, Serranidae) off Peru

    PubMed Central

    Knoff, Marcelo; Cohen, Simone Chinicz; Cárdenas, Melissa Querido; Cárdenas-Callirgos, Jorge M.; Gomes, Delir Corrêa

    2015-01-01

    Pseudorhabdosynochus jeanloui n. sp. (Monogenoidea, Diplectanidae) is described from specimens collected from the gills of the Pacific creolefish, Paranthias colonus (Perciformes, Serranidae) from a fish market in Chorrillos, Lima, Peru. The new species is differentiated from other members of the genus by the structure of its sclerotized vagina, which has two spherical chambers of similar diameter. This is the first Pseudorhabdosynochus species described from the Pacific coast of America, the third species of the genus reported from South America and the first described from a member of Paranthias. PMID:25754099

  19. A new species of diplectanid (Monogenoidea) from Paranthias colonus (Perciformes, Serranidae) off Peru.

    PubMed

    Knoff, Marcelo; Cohen, Simone Chinicz; Cárdenas, Melissa Querido; Cárdenas-Callirgos, Jorge M; Gomes, Delir Corrêa

    2015-01-01

    Pseudorhabdosynochus jeanloui n. sp. (Monogenoidea, Diplectanidae) is described from specimens collected from the gills of the Pacific creolefish, Paranthias colonus (Perciformes, Serranidae) from a fish market in Chorrillos, Lima, Peru. The new species is differentiated from other members of the genus by the structure of its sclerotized vagina, which has two spherical chambers of similar diameter. This is the first Pseudorhabdosynochus species described from the Pacific coast of America, the third species of the genus reported from South America and the first described from a member of Paranthias. © M. Knoff et al., published by EDP Sciences, 2015.

  20. Human health risk assessment of pesticide residues in snappers (Lutjanus) fish from the Navachiste Lagoon complex, Mexico.

    PubMed

    Granados-Galván, Ingrid Alejandra; Rodríguez-Meza, Durga Guadalupe; Luna-González, Antonio; González-Ocampo, Héctor Abelardo

    2015-08-15

    Organochlorine pesticides (OCPs) residues were determined in fillets of Lutjanus colorado, L. argentiventris, and L. novemfasciatus. Fillet samples were collected bimonthly from February 2012 to February 2013. OCPs average concentrations do not differ significantly according to size, weight, or season, nor do they relate with the physico-chemical parameters of the sea water. The highest concentration and most frequently encountered OCPs were endosulfan sulfate, δ-HCH, and heptachlor epoxide, which indicates their use in the recent past and confirms their persistence. Average concentrations of ∑HCHs, ∑chlordane, and ∑heptachlor in samples were above cancer MRLs according to data from monthly consumed portions. HCHs and heptachlor are listed in Appendix III of the Rotterdam Convention of chemicals placed on a prior informed consent procedure for import and export purposes; they are considered illegal in Mexico. The OCPs concentrations above cancer MRLs in Lutjanus spp. turn its frequent consumption into a human health risk. Copyright © 2015 Elsevier Ltd. All rights reserved.

  1. Chromosomal differentiation and speciation in sister-species of Grammatidae (Perciformes) from the Western Atlantic

    NASA Astrophysics Data System (ADS)

    Molina, Wagner Franco; da Costa, Gideão Wagner Werneck Felix; de Bello Cioffi, Marcelo; Bertollo, Luiz Antonio Carlos

    2012-09-01

    In the tropical Atlantic, the ichthyofauna between the coast of Brazil and the Caribbean regions, divided by the Amazon barrier, is very similar presenting several geminate species, including Gramma brasiliensis, endemic in Brazil, and its Caribbean counterpart Gramma loreto. Morphological and molecular studies have helped establish evolutionary patterns that sister-species of these two marine habitats are subjected to. However, their chromosomal characteristics are only beginning to be better characterized. Accordingly, a comparative cytogenetic analysis was carried out in G. brasiliensis and G. loreto, seeking evidence of cytotaxonomic markers implicated in the karyotypic diversification of these species and likely associated with speciation events. Heterochromatic regions and their affinity to fluorochromes GC- or AT-specific were identified, as well as the distribution of ribosomal DNA sites in chromosomes, either by silver nitrate impregnation (Ag-NORs) or dual-color FISH mapping with 18S and 5S rDNA probes. While displaying the same diploid number, 2 n = 48 chromosomes, considered basal for Perciformes, the two species differed in karyotype structure, showing karyotypic formulas and species-specific heterochromatin pattern. The cytological characters found support the differentiating status of these species, possibly achieved under the conditions of allopatry due to the Amazon/Orinoco barrier, showing chromosomal peculiarities in Grammatidae species when compared to other groups of Perciformes.

  2. Concentration of fish serum albumin (FSA) in the aqueous extract of Indonesian Perciformes fishes' muscle tissue.

    PubMed

    Januar, Hedi Indra; Fajarningsih, Nurrahmi Dewi; Zilda, Dewi Seswita; Bramandito, Aditya; Wright, Anthony D

    2015-01-01

    Fish serum albumin (FSA) is an aquatic resource that has potential to be developed as nutraceutical. Therefore, research was undertaken to assess albumin levels in the aqueous extract of muscle tissue of several Perciformes commonly available at a local fish market in Indonesia. Three random replicates for each of 17 Perciformes species were collected and assessed for their FSA content by application of a reversed-phase (C4) HPLC analytical method. Results of these analyses showed that the albumin concentration of the extracts was in the range 3.49-12.61 g/L, and that they varied significantly (P < 0.05) between species and families. This finding may mean that FSA levels are species and family dependent, something that could be investigated in future studies. As fishes from the family Scrombidae showed the highest concentration (12.61 g/L) of FSA, they would likely have the most value as a source for production of albumin-based nutritional and/or clinical products.

  3. Quantitative real-time PCR (qPCR) for the detection and quantification of dactylogyrid parasites infecting Lutjanus guttatus.

    PubMed

    Soler-Jiménez, L C; García-Gasca, A; Fajer-Ávila, E J

    2017-03-07

    Severe infections of the spotted rose snapper Lutjanus guttatus resulting from dactylogyrid monogeneans present a risk to aquaculture. Currently, the diagnosis of this infection requires the morphological identification and manual quantification of parasites. Based on the characterization of the 28S rRNA gene of dactylogyrid species present in L. guttatus, specific primers were designed for real-time polymerase chain reaction (qPCR) using EvaGreen® chemistry. The standard curve method estimated the number of dactylogyrids accurately. A total of 85 gill samples from cage-cultured fish infected with dactylogyrids were analysed. The estimated number of dactylogyrids using this molecular method was very similar to the manual count that was performed initially. The standardized qPCR approach will be helpful as a complementary method for the early routine monitoring of dactylogyrid infections and for epidemiological studies in which a high number of fish must be studied.

  4. Diversification of brain and sense organ morphology in Antarctic dragonfishes (Perciformes: Notothenioidei: Bathydraconidae).

    PubMed

    Eastman, Joseph T; Lannoo, Michael J

    2003-11-01

    In the subzero shelf waters of Antarctica, fishes of the perciform suborder Notothenioidei dominate the fish fauna and constitute an adaptive radiation and a species flock. The 16 species of dragonfishes of the family Bathydraconidae live from surface waters to nearly 3,000 m and have the greatest overall depth range among notothenioid families. We examined the anatomy and histology of the brain, retina, and cephalic lateral line system of nine bathydraconid species representing 8 of the 11 known genera. We evaluate these data against a cladogram identifying three clades in the family. We provide a detailed drawing of the brain and cranial nerves of Gymnodraco acuticeps and Akarotaxis nudiceps. Bathydraconid brain morphology falls into two categories. Brains of most species are similar to those of generalized perciforms and some basal notothenioids (Class I). However, brains of deep-living bathydraconids (members of the tribe Bathydraconini minus Prionodraco) have a reduced telencephalon and tectum that renders the neural axis visible - the stalked brain morphology (Class II). All bathydraconids have duplex (rod and cone) retinae but there is considerable interspecific variation in the ratio of cones:rods and in the number of cells in the internal nuclear layer. Retinal histology reflects habitat depth but is not tightly coupled to phylogeny. Although the deep-living species of Bathydraconini have rod-dominated retinae, the retinae of some sister species are photopic. An expanded cephalic lateral line system is also characteristic of all members of the Bathydraconini as exemplified by Akarotaxis. This morphology includes large lateral line pores, wide membranous canals, hypertrophied canal neuromasts, and large anterodorsal lateral line nerves, eminentia granulares, and crista cerebellares. The saccular otoliths are also enlarged in members of this tribe. Neural diversification among bathydraconids on the Antarctic shelf has not involved the evolution of sensory

  5. Evolutionary analysis of TLR9 genes reveals the positive selection of extant teleosts in Perciformes.

    PubMed

    Zhu, Zhihuang; Sun, Yuena; Wang, Rixin; Xu, Tianjun

    2013-08-01

    The innate immune system can recognize non-self through pattern recognition receptors. Toll-like receptors were the best-known members of these receptors, and they could sense, recognize, and bind pathogen-associated molecular patterns. TLRs played an important role in innate immune system and were conserved in both invertebrate and vertebrate lineages. Thereinto, TLR9 could detect unmethylated CpG motifs in dsDNA and was expected to undergo coevolution with its microbial ligands. It was known that aquatic and terrestrial organisms dwelled in different environments which contained different pathogens, and they had to adapt to their local environmental conditions. Therefore, we collected TLR9 genes from invertebrate to vertebrate to further explore whether the huge differences between aquatic and terrestrial environments affected the TLR9s evolution between aquatic and terrestrial organisms. Molecular evolution analysis detected positively selected sites in the ancestral lineages of vertebrates, teleosts, and Perciformes but not in the ancestral lineage of mammals. In PAML, site model revealed that extant mammalian TLR9 genes underwent positive selection. However, the positive selection of extant teleosts appeared primarily in Perciformes in which there were 14 positively selected sites. Among these sites, two of them were located on the amino acid insertions of the leucine-rich repeats which could create DNA binding sites, three were found on the convex surface which might possibly affect the flexibility of the TLR solenoids, and six were located on the β-face of concave surface which contained the ligand-binding sites of the TLR solenoids. In other ML methods, we also found three sites under selection that coincided with the codons identified by M8 and these sites were all located in LRRs. The diverse aquatic and terrestrial environments might possess different pathogens to make the living organisms adapt to their local environmental conditions. The positive

  6. Chromosome Mapping of Repetitive Sequences in Rachycentron canadum (Perciformes: Rachycentridae): Implications for Karyotypic Evolution and Perspectives for Biotechnological Uses

    PubMed Central

    Jacobina, Uedson Pereira; Cioffi, Marcelo de Bello; Souza, Luiz Gustavo Rodrigues; Calado, Leonardo Luiz; Tavares, Manoel; Manzella, João; Bertollo, Luiz Antonio Carlos; Molina, Wagner Franco

    2011-01-01

    The cobia, Rachycentron canadum, a species of marine fish, has been increasingly used in aquaculture worldwide. It is the only member of the family Rachycentridae (Perciformes) showing wide geographic distribution and phylogenetic patterns still not fully understood. In this study, the species was cytogenetically analyzed by different methodologies, including Ag-NOR and chromomycin A3 (CMA3)/DAPI staining, C-banding, early replication banding (RGB), and in situ fluorescent hybridization with probes for 18S and 5S ribosomal genes and for telomeric sequences (TTAGGG)n. The results obtained allow a detailed chromosomal characterization of the Atlantic population. The chromosome diversification found in the karyotype of the cobia is apparently related to pericentric inversions, the main mechanism associated to the karyotypic evolution of Perciformes. The differential heterochromatin replication patterns found were in part associated to functional genes. Despite maintaining conservative chromosomal characteristics in relation to the basal pattern established for Perciformes, some chromosome pairs in the analyzed population exhibit markers that may be important for cytotaxonomic, population, and biodiversity studies as well as for monitoring the species in question. PMID:21541243

  7. Complete mitochondrial genome of the African pompano Alectis ciliaris (Perciformes: Carangidae).

    PubMed

    Li, Yufang; Chen, Zuozhi; Zhang, Peng; Liang, Peiwen; Chen, Sen; Guo, Yihui; Li, Min

    2016-05-01

    The African pompano Alectis ciliaris (Perciformes: Carangidae) is an economic fish species distributed throughout the tropical oceans and seas of the world. In this study, we assembled the complete mitochondrial genome of A. ciliaris from contiguous, overlapping segments amplified by polymerase chain reactions. The complete mitogenome sequence was 16,570 bp in length, consisting of 37 typical animal mitochondrial genes and 1 control region, same with the typical vertebrate mitochondrial gene arrangement. There were 10 regions of gene overlaps totaling 30 bp and 12 intergenic spacer regions totaling 67 bp. The overall base composition of the heavy strand was 28.32% for A, 26.77% for T, 16.16% for G, 28.75% for C with a slight AT bias of 55.09%.

  8. The complete mitochondrial genome of the Blue-face angelfish, Pomacanthus xanthometapon (Perciformes: Pomacanthidae).

    PubMed

    Shen, Kang-Ning; Loh, Kar-Hoe; Chen, Ching-Hung; Hsiao, Chung-Der

    2016-11-01

    In this study, the complete mitogenome sequence of the Blue-face angelfish, Pomacanthus xanthometapon (Perciformes: Pomacanthidae) has been sequenced by the next-generation sequencing method. The assembled mitogenome consisting of 16,533 bp includes 13 protein coding genes, 22 transfer RNAs, and two ribosomal RNAs genes. The overall base composition of Blue-face angelfish is 28.7% for A, 28.9% for C, 15.9% for G, 26.6% for T and show 84% identities to flame angelfish Centropyge loriculus. The complete mitogenome of the Blue-face angelfish provides essential and important DNA molecular data for further phylogeography and evolutionary analysis for marine angelfish phylogeny.

  9. Two gonad-infecting species of Philometra (Nematoda: Philometridae) from marine fishes off the northern coast of Australia

    PubMed Central

    Moravec, František; Barton, Diane P.

    2015-01-01

    Two different gonad-infecting species of Philometra Costa, 1845 were collected from the ovary of marine perciform fishes, the blackspotted croaker Protonibea diacanthus (Sciaenidae) and the John’s snapper Lutjanus johnii (Lutjanidae), from off the northern coast of Australia. Nematodes (males and females) from P. diacanthus represent a new taxon, Philometra protonibeae n. sp., which is mainly characterized by the body length of the males (3.37–3. 90 mm), broad, equally long spicules (length 126–141 μm) and the shape and structure of the gubernaculum with a dorsally lamellate distal tip. The nematodes (only females) from L. johnii may represent an undescribed species, but, because of the absence of conspecific males, they could not be specifically identified. Philometra protonibeae is the fifth nominal gonad-infecting species of this genus recorded from marine fishes in Australian waters and the seventh species of these parasites described from fishes of the family Sciaenidae. PMID:25654578

  10. Philometrid nematodes infecting fishes from the Everglades National Park, Florida, U.S.A.

    PubMed

    Moravec, Frantisek; Bakenhaster, Micah

    2010-09-01

    The following three species of the Philometridae (Nematoda: Dracunculoidea) are described from marine perciform fishes of the Everglades National Park (northern Gulf of Mexico), Florida, U.S.A.: Philometra brevispicula sp. n. (male and females) from the subcutaneous tissue of mouth of the gray snapper Lutjanus griseus (Linnaeus) (Lutjanidae), Philometroides grandipapillatus sp. n. (only females) from pectoral fin muscle of the crevalle jack Caranx hippos (Linnaeus) (Carangidae), and Caranginema americanum Moravec, Montoya-Mendoza et Salgado-Maldonado, 2008 (females) from the subcutaneous fascia of trunk muscle in crevalle jack C. hippos. Philometra brevispicula is mainly characterized by small cephalic papillae of the external circle, the absence of oesophageal teeth and the presence of small caudal projections in gravid female, markedly short spicules (45 microm) in male, and by its location in the host. Philometroides grandipapillatus differs from congeners mainly in the shape of the cephalic region (narrow, conspicuously protruding), large cephalic papillae of the external circle and the absence of caudal projections in female, and by the site of infection in the host. Caranginema americanum is for the first time recorded from the northern Gulf of Mexico.

  11. Cross-continent comparisons reveal differing environmental drivers of growth of the coral reef fish, Lutjanus bohar

    NASA Astrophysics Data System (ADS)

    Ong, Joyce J. L.; Rountrey, Adam N.; Marriott, Ross J.; Newman, Stephen J.; Meeuwig, Jessica J.; Meekan, Mark G.

    2017-03-01

    Biochronologies provide important insights into the growth responses of fishes to past variability in physical and biological environments and, in so doing, allow modelling of likely responses to climate change in the future. We examined spatial variability in the key drivers of inter-annual growth patterns of a widespread, tropical snapper, Lutjanus bohar, at similar tropical latitudes on the north-western and north-eastern coasts of the continent of Australia. For this study, we developed biochronologies from otoliths that provided proxies of somatic growth and these were analysed using mixed-effects models to examine the historical drivers of growth. Our analyses demonstrated that growth patterns of fish were driven by different climatic and biological factors in each region, including Pacific Ocean climate indices, regional sea level and the size structure of the fish community. Our results showed that the local oceanographic and biological context of reef systems strongly influenced the growth of L. bohar and that a single age-related growth trend cannot be assumed for separate populations of this species that are likely to experience different environmental conditions. Generalised predictions about the growth response of fishes to climate change will thus require adequate characterisation of the spatial variability in growth determinants likely to be found throughout the range of species that have cosmopolitan distributions.

  12. Characterization and expression analysis of B Cell receptor accessory molecule CD79 gene in humphead snapper ( Lutjanus sanguineus)

    NASA Astrophysics Data System (ADS)

    Huang, Yucong; Yan, Xiuying; Cai, Shuanghu; Cai, Jia; Jian, Jichang; Lu, Yishan; Tang, Jufen; Wu, Zaohe

    2016-04-01

    CD79, a key component of the B cell antigen receptor complex, is composed of CD79α(Igα) and CD79β(Igβ) encoded by mb-1 and B29 respectively, and plays an important role in B cell signaling. In this study, we isolated and characterized mb-1 and B29 from humphead snapper ( Lutjanus sanguineus). Their tissue distribution and expression profiles after stimulations in vitro and in vivo were also investigated. The humphead snapper mb-1 and B29 contain open reading frames of 684 bp and 606 bp, encoding 227 amino acids and 201 amino acids, respectively. Both CD79α and CD79β possess signal peptide, extracellular Ig domain, transmembrane region and immunoreceptor tyrosine kinase activation motif (ITAM). Mb-1 is highly expressed in lymphoid organs (thymus, posterior kidney and spleen) and mucosal-associated lymphoid tissues (gill and intestine), while B29 is mainly detected in posterior kidney, spleen, gill and skin. Furthermore, transcription of mb-1 and B29 in head kidney leucocytes was up-regulated following lipopolysaccharide (LPS), pokeweed mitogen (PWM), and polyinosinic-polycytidylic acid (PolyI:C) stimulation, respectively, and their expression level in anterior kidney and spleen was also increased after challenged with formalin-inactived Vibrio harveyi. These results indicated that humphead snapper CD79 molecule might play an important role in immune response to pathogen infection.

  13. Morphology and phylogeny of Henneguya jocu n. sp. (Myxosporea, Myxobolidae), infecting the gills of the marine fish Lutjanus jocu.

    PubMed

    Azevedo, Carlos; Rocha, Sónia; Matos, Patrícia; Matos, Edilson; Oliveira, Elsa; Al-Quraishy, Saleh; Casal, Graça

    2014-04-01

    Henneguya jocu n. sp. (Myxosporea, Myxobolidae) is described from the gill lamellae of the marine teleost fish Lutjanus jocu, with a focus on ultrastructural and molecular features. This myxosporean forms subspherical cysts up to ∼260 μm × 130 μm long, and develops asynchronously. Mature myxospores ellipsoidal with a bifurcated caudal process. Myxospore length 10.9 ± 0.4 μm (n=50); width, 8.2 ± 0.3 μm (n=50); and thickness, 2.9 ± 0.5 μm (n=50). Two equal caudal processes, 34.1±1.0 μm long (n=50); and total myxospore length, 45.2 ± 1.0 μm (n=50). Two symmetric valves surround two ellipsoidal polar capsules, 5.0 ± 0.3 × 1.4 ± 0.2 μm (n=20), each containing an isofilar polar filament forming 4-5 coils along the inner wall of these structures, as well as a binucleated sporoplasm presenting a spherical vacuole and several globular sporoplasmosomes. Both the morphological data and molecular analysis of the SSU rDNA gene identify this parasite as a new species of the genus Henneguya. Maximum Likelihood and Maximum Parsimony analyses further indicate that the parasite clusters within others marine Myxobolidae species, forming a group alongside other Henneguya species described from marine hosts. Copyright © 2013 Elsevier GmbH. All rights reserved.

  14. Spatial and Dietary Overlap Creates Potential for Competition between Red Snapper (Lutjanus campechanus) and Vermilion snapper (Rhomboplites aurorubens)

    PubMed Central

    Davis, William T.; Drymon, J. Marcus; Powers, Sean P.

    2015-01-01

    Understanding the complex nature of direct and indirect species interactions is a critical precursor to successful resource management. In the northern Gulf of Mexico fisheries ecosystem, red snapper (Lutjanus campechanus) and vermilion snapper (Rhomboplites aurorubens) are two commercially harvested species within a larger reef fish complex. These two species share similar habitats and diets; however, little is known about how these species partition habitat and dietary resources. In this study we examined the extent of spatial and dietary overlap between red snapper and vermilion snapper, and experimentally compared their feeding behavior. Field data from multiple gear types demonstrates that red snapper and vermilion snapper frequently cohabited reefs in the northern Gulf of Mexico, and Pianka’s niche overlap indices suggest significantly overlapping diets. Experimental manipulations show that red snapper are the dominant forager of the two species, as red snapper foraging alone ate more shrimp per fish than vermilion snapper in both the single species (p = 0.003) and mixed species (p = 0.02) treatments. In addition, red snapper ate significantly more shrimp per fish in the mixed species treatment than in the single species treatment (p = 0.04). Vermilion snapper shrimp consumption per fish did not differ significantly between mixed and single species treatments. Cumulatively, our results suggest that spatial and dietary overlap could lead to competition between red and vermilion snapper in the study area; however, conclusively determining the existence of such competition would require further research. PMID:26630481

  15. Spatial and Dietary Overlap Creates Potential for Competition between Red Snapper (Lutjanus campechanus) and Vermilion snapper (Rhomboplites aurorubens).

    PubMed

    Davis, William T; Drymon, J Marcus; Powers, Sean P

    2015-01-01

    Understanding the complex nature of direct and indirect species interactions is a critical precursor to successful resource management. In the northern Gulf of Mexico fisheries ecosystem, red snapper (Lutjanus campechanus) and vermilion snapper (Rhomboplites aurorubens) are two commercially harvested species within a larger reef fish complex. These two species share similar habitats and diets; however, little is known about how these species partition habitat and dietary resources. In this study we examined the extent of spatial and dietary overlap between red snapper and vermilion snapper, and experimentally compared their feeding behavior. Field data from multiple gear types demonstrates that red snapper and vermilion snapper frequently cohabited reefs in the northern Gulf of Mexico, and Pianka's niche overlap indices suggest significantly overlapping diets. Experimental manipulations show that red snapper are the dominant forager of the two species, as red snapper foraging alone ate more shrimp per fish than vermilion snapper in both the single species (p = 0.003) and mixed species (p = 0.02) treatments. In addition, red snapper ate significantly more shrimp per fish in the mixed species treatment than in the single species treatment (p = 0.04). Vermilion snapper shrimp consumption per fish did not differ significantly between mixed and single species treatments. Cumulatively, our results suggest that spatial and dietary overlap could lead to competition between red and vermilion snapper in the study area; however, conclusively determining the existence of such competition would require further research.

  16. Trophic and reproductive ecology of Red Snapper, Lutjanus campechanus, on natural and artificial reefs in the western Gulf of Mexico

    NASA Astrophysics Data System (ADS)

    Ajemian, M. J.; Wetz, J. J.; Brewton, R. A.; Downey, C. H.; Stunz, G. W.

    2016-02-01

    Energy exploration in the Gulf of Mexico (Gulf) has resulted in the addition of numerous oil and gas production rigs that have added structurally complex habitat to an area otherwise dominated by bare bottom. The impact of these artificial structures on fish populations is largely unknown and there is ongoing debate about their functionality. Red Snapper (Lutjanus campechanus), an ecologically and economically important sportfish to the region, use natural as well as the artificial reefs created by standing and reefed (toppled or cutoff) oil and gas rigs. However, little is known about how trophic and reproductive characteristics of Red Snapper vary over these multiple habitat types. We analyzed stable isotopic composition (δ13C, δ 15N) of epaxial muscle and compared reproductive potential of Red Snapper (155-855 mm TL) from standing rigs, reefed rigs, and natural hard-bottom habitats off Texas. Red Snapper from standing rig sites were isotopically enriched in δ 15N compared to lower relief habitats, suggesting they were feeding at a higher trophic level on standing rigs. While gonadosomatic indices (GSI) and comparative histology implied a similar spawning season among structure types, GSI was highest for both sexes at standing rigs. These initial results suggest that while standing rigs appear to provide more enriched food resources leading to higher Red Snapper reproductive capacity, the productivity of this species is similar between currently permitted rig decommissioning options (i.e., cutoff and toppled rigs) and natural hard-bottom habitats in the Gulf of Mexico.

  17. Molecular cloning and characterization of high mobility group box1 (Ls-HMGB1) from humphead snapper, Lutjanus sanguineus.

    PubMed

    Cai, Jia; Xia, Hongli; Huang, Yucong; Lu, Yishan; Wu, Zaohe; Jian, Jichang

    2014-10-01

    High mobility group box1 (HMGB1) is a kind of chromatin-associated nonhistone protein important for nucleosome formation, transcriptional regulation and inflammation. However, the reports about HMGB1 of marine fish were still limited. Here, we cloned and characterized a HMGB1 gene from humphead snapper, Lutjanus sanguineus (Ls-HMGB1). The Ls-HMGB1 cDNA composed of 1199 bp with a 70 bp of 5'-UTR, 630 bp open reading frame (ORF) and 499 bp 3'-UTR, encoded a polypeptide of 210 amino acids (GenBank Accession No: KJ783442). Sequence alignment of Ls-HMGB1 showed the highest similarity of 91% with Sciaenops ocellatus HMGB1 protein. Quantitative real-time PCR (qRT-PCR) analysis revealed that Ls-HMGB1 had relatively high expression level in skin, kidney and heart. After Vibrio harveyi and poly I:C stimulation, transcripts of Ls-HMGB1 were significantly increased and reached to peak at 18 h p.i. The L. sanguineus interleukin-6 (Ls-IL6) transcription in HK leukocytes was significantly induced by recombinant LsHMGB1 (rLsHMGB1). These results indicated that Ls-HMGB1 may play an important role in immune response of L. sanguineus during pathogen challenge.

  18. Morphological Characters and Transcriptome Profiles Associated with Black Skin and Red Skin in Crimson Snapper (Lutjanus erythropterus)

    PubMed Central

    Zhang, Yan-Ping; Wang, Zhong-Duo; Guo, Yu-Song; Liu, Li; Yu, Juan; Zhang, Shun; Liu, Shao-Jun; Liu, Chu-Wu

    2015-01-01

    In this study, morphology observation and illumina sequencing were performed on two different coloration skins of crimson snapper (Lutjanus erythropterus), the black zone and the red zone. Three types of chromatophores, melanophores, iridophores and xanthophores, were organized in the skins. The main differences between the two colorations were in the amount and distribution of the three chromatophores. After comparing the two transcriptomes, 9200 unigenes with significantly different expressions (ratio change ≥ 2 and q-value ≤ 0.05) were found, of which 5972 were up-regulated in black skin and 3228 were up-regulated in red skin. Through the function annotation, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the differentially transcribed genes, we excavated a number of uncharacterized candidate pigment genes as well as found the conserved genes affecting pigmentation in crimson snapper. The patterns of expression of 14 pigment genes were confirmed by the Quantitative real-time PCR analysis between the two color skins. Overall, this study shows a global survey of the morphological characters and transcriptome analysis of the different coloration skins in crimson snapper, and provides valuable cellular and genetic information to uncover the mechanism of the formation of pigment patterns in snappers. PMID:26569232

  19. Expression and activity of trypsin and pepsin during larval development of the spotted rose snapper Lutjanus guttatus.

    PubMed

    Galaviz, Mario A; García-Ortega, Armando; Gisbert, Enric; López, Lus M; Gasca, Alejandra García

    2012-01-01

    The present study aimed to describe and understand the development of the digestive system in spotted rose snapper (Lutjanus guttatus) larvae from hatching to 40 days post-hatch (dph). The mouth opened between 2 and 3 dph, at that moment the digestive tract was barely differentiated into the anterior and posterior intestine, although the liver and pancreas were already present. Gastric glands were observed until 20 dph, followed by the differentiation of the stomach between 20 and 25 dph. Trypsinogen expression and trypsin activity were detected at hatching, increasing concomitantly to larval development and the change in the type of food. Maximum levels of trypsinogen expression were observed at 25 dph, when animals were fed with Artemia nauplii, and maximum trypsin activity was detected at 35 dph, when larvae were fed with an artificial diet. On the other hand, pepsinogen gene expression was detected at 18 dph, two days before pepsin enzymatic activity and appearance of gastric glands. Maximum pepsin activity was also observed at 35 dph. These results suggest that in this species weaning could be initiated at an earlier age than is currently practiced (between 28 and 30 dph), since larvae of spotted rose snapper develop a functional stomach between days 20 and 25 post-hatch. Copyright © 2011 Elsevier Inc. All rights reserved.

  20. Ningalooia psammopercae n. g., n. sp. (Digenea: Acanthocolpidae) from the Waigieu seaperch Psammoperca waigiensis (Cuvier) (Perciformes: Latidae) on the Ningaloo Reef, Western Australia.

    PubMed

    Bray, Rodney A; Cribb, Thomas H

    2007-02-01

    Ningalooia psammopercae n. g., n. sp. is described from the intestine of the Waigieu seaperch Psammoperca waigiensis Cuvier (Perciformes: Latidae) on the Ningaloo Reef, Western Australia. The new genus and species are distinctive within the Acanthocolpidae for the combination of the absence of enlarged spines around the oral sucker, a blind ending H-shaped intestine and a deeply lobed ovary.

  1. Anatomical and surface morphology of Hedruris lutjanenses sp. n. (Nematoda: Hedruridae) from the common marinewater fish Lutjanus synagris in Damietta, Egypt.

    PubMed

    Ramadan, M M; Awad, E R; Taha, R G

    2014-08-01

    The description and tegumental ultrastructure of nematode Hedruris lutjanenses sp. n. collected from marine Lane Snapper fish Lutjanus synagris Linnaeus, 1758 collected at Manzala Lake, Damietta, Egypt was studied by light and Scanning Electron Microscopy (SEM). The new species is mainly characterized by that the buccal capsule is surrounded by four pseudolapia; one small and three larger pseudolapia, each one bears a number of papillae arranged irregularly on its surface and the presence of one pair of precloacal papillae and nine pairs of postcloacal ones in males.

  2. Complete mitochondrial genome of the masked spinefoot Siganus puellus (Perciformes, Siganidae).

    PubMed

    Wang, Ming; Yan, Shuai; Li, Min; Zhao, Ming; Yang, Tingbao

    2015-01-01

    In the present study, the complete mitochondrial genome of the masked spinefoot Siganus puellus (Perciformes, Siganidae) is determined. The entire sequence is 16,504 bp in length, including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region. Gene organization and gene order are similar to those found in other rabbitfishes. Apart from ND6 and eight tRNA genes, all other mitochondrial genes are encoded on the heavy strand. Overall base compositions of mitogenome are 28.6% of A, 29.5% of C, 25.6% of T and 16.3% of G. The overall mean genetic distances of 13 protein-coding genes among three Siganus species with known complete mitogenome ranges from 0.076 (COXII) to 0.154 (ATP6), higher than the divergence (0.107) of control region, which implies that ATP6 gene may be more appropriate for population genetic structure analyses of Siganus species.

  3. Complete mitochondrial genome of the orange-spotted spinefoot Siganus guttatus (Perciformes, Siganidae).

    PubMed

    Yan, Shuai; Wang, Ming; Yang, Chaopin; Yang, Tingbao

    2016-01-01

    The complete mitochondrial genome of the orange-spotted spinefoot Siganus guttatus (Perciformes, Siganidae) is presented in this paper. The entire sequence, 16,505 bp in length, is comprised of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 control region. Structurally, the composition and order of genes are same as those found in other rabbitfishes. Except ND6 and 8 tRNA, all other mitochondrial genes were encoded on the heavy strand. Overall base compositions of mitogenome are 29.3% of A, 29.3% of C, 25.7% of T, and 15.7% of G, showing an obvious anti-G bias which was commonly found in fishes. Three conserved sequence blocks (CSB-1, CSB-2 and CSB-3) and long series of di- and mono-nucleotide microsatellite repeats in 3' end are observed in the putative control region. The complete mitogenome sequence of S. guttatus could provide useful information for further phylogenetic and population genetic studies.

  4. Isolation and characterization of type I antifreeze proteins from cunner, Tautogolabrus adspersus, order Perciformes.

    PubMed

    Hobbs, Rod S; Shears, Margaret A; Graham, Laurie A; Davies, Peter L; Fletcher, Garth L

    2011-10-01

    Antifreeze proteins (AFPs) are produced by many species of teleost fish that inhabit potentially lethal ice-laden seawater and afford them protection from freezing. To date type I AFPs have been fully characterized in two teleost orders: Pleuronectiformes and Scorpaeniformes. In this study, we report the isolation and complete characterization of a type I AFP present in fish from a third order: cunner (Tautogolabrus adspersus), order Perciformes (family Labridae). This protein was purified from blood plasma and found to belong to what is now known as classical type I AFP with their small size (mass 4095.16 Da), alanine richness (> 57 mol%), high α-helicity (> 99%) with the ability to undergo reversible thermal denaturation, 11 amino acid (ThrX(10)) repeat regions within the primary structure, the capacity to impart a hexagonal bipyramidal shaping to ice crystals and the conservation of an ice-binding site found in many of the other type I AFPs. Partial de novo sequencing of the plasma AFP accounted for approximately half of the peptide mass. Sequencing of a combined liver and skin cDNA library indicated that the protein is produced without a signal sequence. In addition the translated product of the AFP cDNA suggests that it codes for the AFP isolated from plasma. These results further solidify the hypothesis that type I AFPs are multiphyletic in origin and suggest that they represent remarkable examples of convergent evolution within three orders of teleost fish.

  5. Organization and characterization of the mitochondrial genome of the Razorbelly scad Alepes kleinii (Perciformes: Carangidae).

    PubMed

    Li, Min; Zou, Keshu

    2016-01-01

    The complete mitochondrial genome sequence of Alepes kleinii (Perciformes: Carangidae) was determined in this study. The entire sequence was 16,571 bp in length which contained 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 2 non-coding regions (the control region and the light strand replication origin). Gene organization and gene order were similar to that observed in most other vertebrates. Overall base compositions of the sequence were 28.0% of A, 28.5% of C, 27.0% of T, and 16.5% of G, showing an obvious anti-G bias commonly observed in teleosts. Apart from the ND6 and eight tRNA genes, all other genes were encoded on the heavy strand. Three types of initiation codons were detected in the open reading frames of the protein-coding genes, including codon ATA which was rarely detected in Carangidae species. The complete mitogenome sequence data of A. kleinii could provide useful information for taxonomic and phylogenetics studies.

  6. Assessment of the acute toxicity of the organochlorine pesticide endosulfan in Cichlasoma dimerus (Teleostei, Perciformes).

    PubMed

    Da Cuña, Rodrigo Hernán; Rey Vázquez, Graciela; Piol, María Natalia; Guerrero, Noemí Verrengia; Maggese, María Cristina; Lo Nostro, Fabiana Laura

    2011-05-01

    The organochlorine insecticide endosulfan (ES) is widely used despite its high toxicity to fish (96-h LC(50) median value of 2.6 μg L(-1)). This study aimed to assess the acute toxicity, histological and physiological parameters after exposure to 0; 0.25; 1; 2; 3; 4 and 16 μg L(-1) ES for 96 h under semi-static conditions in a freshwater perciform fish, Cichlasoma dimerus. Prior to death, fish exhibited behavior indicative of neurotoxicity. No difference was found in brain AChE activity. A decrease in erythrocyte mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration in exposed fish suggests a state of anemia. Histological alterations observed in exposed fish included hyperplasia of the interlamellar epithelium, blood congestion in secondary lamellae, and mucous cells hyperplasia and hypertrophy in gills; pycnotic nuclei and hydropic degeneration in liver; testicular damage. These moderate pathological responses in major organs could become crucial during reproduction and under prolonged exposure periods. Copyright © 2011 Elsevier Inc. All rights reserved.

  7. Discovery of toll-like receptor 13 exists in the teleost fish: Miiuy croaker (Perciformes, Sciaenidae).

    PubMed

    Wang, Yanjin; Bi, Xueyi; Chu, Qing; Xu, Tianjun

    2016-08-01

    Toll-like receptors (TLRs) play an indispensable role in the immune response for pathogen recognition and triggering not only innate immunity but also adaptive immunity. Here we report the TLR13 homologue, one member of TLRs, in Perciformes (especially Sciaenidae). And we used the miiuy croaker as represented species for further functional experiments. Former study reported the TLR13 only expressed in murine, and we are the first to report the teleost TLR13 (mmiTLR13). MmiTLR13 expressed highly in immune defense related tissues, such as the liver, spleen, and kidney, and Vibrio anguillarum or poly(I:C) infection showed the immune response of mmiTLR13. Further luciferase reporter assays showed the ability for activation of ISRE luciferase reporter, but it failed to active NF-κB. And further gene silence by short hairpin RNA (shRNA) confirmed the results. Immunofluorescence of mmiTLR13 presents the cytoplasmic distribution in Hela cell. In addition, the Toll/interleukin 1 receptor (TIR) domain of mammal TLR5 exhibits high identity with TLR13, which indicated the high homology between TLR5 and TLR13. These findings will lay the fundamental cornerstone for further research of teleost TLR13 and expand the horizon for better understand the teleost TLRs system. Copyright © 2016 Elsevier Ltd. All rights reserved.

  8. Molecular cytogenetic analyses of Epinephelus bruneus and Epinephelus moara (Perciformes, Epinephelidae).

    PubMed

    Guo, Minglan; Wang, Shifeng; Su, Yongquan; Zhou, Yongcan; Liu, Min; Wang, Jun

    2014-01-01

    Genus Epinephelus (Perciformes, Epinephelidae), commonly known as groupers, are usually difficult in species identification for the lack and/or change of morphological specialization. In this study, molecular cytogenetic analyses were firstly performed to identify the closely related species Epinephelus bruneus and E. moara in this genus. The species-specific differences of both fish species showed in karyotype, chromosomal distribution of nucleolar organizer regions (NORs) and localization of 18S rDNA. The heterochromatin (interstitial C-bands) and distribution pattern of telomere (TTAGGG) n in E. bruneus revealed the chromosomal rearrangements and different karyotypic evolutionary characteristics compared to those in E. moara. The cytogenetic data suggested that the lineages of E. bruneus and E. moara were recently derived within the genus Epinephelus, and E. moara exhibited more plesiomorphic features than E. bruneus. All results confirmed that E. moara, which has long been considered a synonym of E. bruneus, is a distinct species in the family Epinephelidae. In addition, molecular cytogenetic analyses are useful in species differentiation and phylogenetic reconstruction in groupers.

  9. The complete mitochondrial genome of two recently derived species of the fish genus Nannoperca (Perciformes, Percichthyidae).

    PubMed

    Prosdocimi, Francisco; de Carvalho, Daniel Cardoso; de Almeida, Renan Nascimento; Beheregaray, Luciano B

    2012-03-01

    Here we report the complete sequence of mitochondrial genomes for two sister taxa of freshwater teleosts, the recently derived Yarra pigmy perch Nannoperca obscura and the southern pigmy perch Nannoperca australis. These represent the first complete mitochondrial genomes for Percichthyidae (Perciformes), a family mostly distributed in Australia. The de novo genome assembly of 316,430 pyrosequencing reads from 454 libraries has produced the entire mitochondria for N. obscura and a nearly complete version for N. australis. The mtDNA genome from the latter was completed through the design of one primer set and standard Sanger sequencing for genome finishing, followed by the hybrid assembly of reads with MIRA software using N. obscura sequence as reference genome. The complete mitogenomes of N. obscura and N. australis are 16,496 and 16,494 bp in size, respectively. Both genomes contain 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and a control region. Several characteristics of mitochondria typically found in teleost fishes were detected, such as: (i) most genes found in the heavy strand, with the exception of ND6 and eight tRNA genes; (ii) avoidance of G as the third base of codons; (iii) presence of gene overlapping; (iv) percentage of bases usage. We found only eight indels and 197 nucleotide substitutions between these Nannoperca mitogenomes, consistent with a previous hypothesis of recent speciation. The data reported here provide a resource for comparative analysis of recent evolution of mitochondrial genomes.

  10. Regional variation in mercury and stable isotopes of red snapper (Lutjanus campechanus) in the northern Gulf of Mexico, USA.

    PubMed

    Zapp Sluis, Michelle; Boswell, Kevin M; Chumchal, Matthew M; Wells, R J David; Soulen, Brianne; Cowan, James H

    2013-02-01

    The presence of total mercury (Hg) in fish tissue and the potential associated health risks has become a global concern in marine ecosystems. Few studies have examined basin-scale variation in Hg accumulation in marine ecosystems, and determining if Hg concentrations in fish tissue vary across marine ecosystems is a key monitoring question. The present study evaluated Hg concentrations in red snapper (Lutjanus campechanus) tissue across three regions of the northern Gulf of Mexico (Alabama, Louisiana, and Texas, USA) and between two habitat types (oil and gas platforms and nonplatforms) within each region. Nitrogen (δ(15)N), carbon (δ(13)C), and sulfur (δ(34)S) stable isotopes were used to investigate ecological differences that may affect Hg concentrations among regions and between habitats. Mercury concentrations in red snapper tissue were positively correlated with fish total length. Regional differences in Hg concentrations were significant, with fish collected from Alabama having the highest concentrations and fish collected from Louisiana having the lowest. No significant difference existed in Hg concentrations between habitats, suggesting that association with platforms may not be a significant factor contributing to red snapper Hg concentrations. While δ(15)N did not differ significantly among the three regions, Texas red snapper were more enriched in δ(34)S and depleted in δ(13)C compared with Alabama and Louisiana red snapper. Although the majority of red snapper collected in the present study had Hg concentrations below safe consumption guidelines, regional differences suggest that spatially explicit monitoring programs may be important for basin-wide assessments.

  11. Metal contents in the tissues of Lutjanus fulviflamma (Smith 1949) and Epinephelus tauvina (Forskal 1775) collected from the Arabian Gulf

    SciTech Connect

    Ahmad, S.; Al-Ghais, S.M.

    1996-12-01

    The role heavy metals (the non-degradable and commutative chemicals) play as pollutants is widely recognized. In the sea, accumulation of pollutants may cause the toxicity to the aquatic organism and subsequently transferred to man through the food chain. Among the major sources of metal contamination are industrial activities and mining. Natural processes such as volcanic eruptions, erosion and wind are also important. At many places, industrial and agricultural discharges were found primary source of metal poisoning of fish e.g. Poland, Canada. Highway or motorboat traffic has also been reported as a major contributor of the problem. Exposure to heavy metals through air, water and/or the food chain is known to induce a wide variety of toxic effects in humans and animals. Some of these heavy metals are considered as essential elements for normal physiological functions of the human as well as for the most of animals micronutrients but the higher levels may be toxic or harmful. Extensive studies have been carried out in many parts of the world to determine toxicity and bio-accumulation of these metals in fish and other marine flora and fauna. However, there is a gap in our knowledge of the kind and extent of marine pollution by heavy metals around the coast of the United Arab Emirates (UAE) and the resultant contamination of the aquatic habitat. In continuation of our interest in marine environment, this study was designed to investigate and to compare the concentrations of cadmium, copper, manganese, nickel, lead and zinc in the skin, muscle and liver, and mercury in the muscle tissues only of Lutjanus fulviflamma and Epinephelus tauvina. These species have been selected as these are among the highly commercial fish species found in the trap fishery of United Arab Emirates. 25 refs., 3 figs., 5 tabs.

  12. Characterization and expression of MHC class II alpha and II beta genes in mangrove red snapper (Lutjanus argentimaculatus).

    PubMed

    Wang, Tianyan; Tan, Shangjin; Cai, Zhonghua

    2015-12-01

    The major histocompatibility complex (MHC) class II plays a key role in adaptive immunity by presenting foreign peptides to CD4(+) T cells and by triggering the adaptive immune response. While the structure and function of MHC class II have been well characterized in mammalian, limited research has been done on fishes. In this study, we characterized the gene structure and expression of MHC class II α (Lunar-DAA) and II β (Lunar-DAB) of mangrove red snapper (Lutjanus argentimaculatus). Both genes shared, respectively, a high similarity and typical features with other vertebrate MHC class II α and II β. The phylogenetic analysis of the deduced peptides revealed that both Lunar-DAA and Lunar-DAB were located in the teleost subclass. Western blotting analyses indicated that both MHC class II α and II β were expressed ubiquitously in immune-related cells, tissues and organs, and that MHC class II α and II β chains existed mainly as heterodimers. While it was highly expressed in gills, thymus, head kidney (HK), spleen, head kidney macrophage and spleen leucocytes, MHC class II β chain was expressed with a low abundance in skin, intestine, stomach and heart. The highest expression of MHC class II β in thymus confirmed the conclusion that thymus is one of the primary lymphoid organs in fishes. The detection of MHC class II αβ dimers in HK macrophages and spleen leucocytes indicated that HK macrophages and spleen leucocytes play a critical role in the adaptive immunity in fishes. All these results provide valuable information for understanding the structure of MHC class II α and II β and their function in immune responses.

  13. An annotated list of fish parasites (Isopoda, Copepoda, Monogenea, Digenea, Cestoda, Nematoda) collected from Snappers and Bream (Lutjanidae, Nemipteridae, Caesionidae) in New Caledonia confirms high parasite biodiversity on coral reef fish

    PubMed Central

    2012-01-01

    Background Coral reefs are areas of maximum biodiversity, but the parasites of coral reef fishes, and especially their species richness, are not well known. Over an 8-year period, parasites were collected from 24 species of Lutjanidae, Nemipteridae and Caesionidae off New Caledonia, South Pacific. Results Host-parasite and parasite-host lists are provided, with a total of 207 host-parasite combinations and 58 parasite species identified at the species level, with 27 new host records. Results are presented for isopods, copepods, monogeneans, digeneans, cestodes and nematodes. When results are restricted to well-sampled reef fish species (sample size > 30), the number of host-parasite combinations is 20–25 per fish species, and the number of parasites identified at the species level is 9–13 per fish species. Lutjanids include reef-associated fish and deeper sea fish from the outer slopes of the coral reef: fish from both milieus were compared. Surprisingly, parasite biodiversity was higher in deeper sea fish than in reef fish (host-parasite combinations: 12.50 vs 10.13, number of species per fish 3.75 vs 3.00); however, we identified four biases which diminish the validity of this comparison. Finally, these results and previously published results allow us to propose a generalization of parasite biodiversity for four major families of reef-associated fishes (Lutjanidae, Nemipteridae, Serranidae and Lethrinidae): well-sampled fish have a mean of 20 host-parasite combinations per fish species, and the number of parasites identified at the species level is 10 per fish species. Conclusions Since all precautions have been taken to minimize taxon numbers, it is safe to affirm than the number of fish parasites is at least ten times the number of fish species in coral reefs, for species of similar size or larger than the species in the four families studied; this is a major improvement to our estimate of biodiversity in coral reefs. Our results suggest that

  14. An annotated list of fish parasites (Isopoda, Copepoda, Monogenea, Digenea, Cestoda, Nematoda) collected from Snappers and Bream (Lutjanidae, Nemipteridae, Caesionidae) in New Caledonia confirms high parasite biodiversity on coral reef fish.

    PubMed

    Justine, Jean-Lou; Beveridge, Ian; Boxshall, Geoffrey A; Bray, Rodney A; Miller, Terrence L; Moravec, František; Trilles, Jean-Paul; Whittington, Ian D

    2012-09-04

    Coral reefs are areas of maximum biodiversity, but the parasites of coral reef fishes, and especially their species richness, are not well known. Over an 8-year period, parasites were collected from 24 species of Lutjanidae, Nemipteridae and Caesionidae off New Caledonia, South Pacific. Host-parasite and parasite-host lists are provided, with a total of 207 host-parasite combinations and 58 parasite species identified at the species level, with 27 new host records. Results are presented for isopods, copepods, monogeneans, digeneans, cestodes and nematodes. When results are restricted to well-sampled reef fish species (sample size > 30), the number of host-parasite combinations is 20-25 per fish species, and the number of parasites identified at the species level is 9-13 per fish species. Lutjanids include reef-associated fish and deeper sea fish from the outer slopes of the coral reef: fish from both milieus were compared. Surprisingly, parasite biodiversity was higher in deeper sea fish than in reef fish (host-parasite combinations: 12.50 vs 10.13, number of species per fish 3.75 vs 3.00); however, we identified four biases which diminish the validity of this comparison. Finally, these results and previously published results allow us to propose a generalization of parasite biodiversity for four major families of reef-associated fishes (Lutjanidae, Nemipteridae, Serranidae and Lethrinidae): well-sampled fish have a mean of 20 host-parasite combinations per fish species, and the number of parasites identified at the species level is 10 per fish species. Since all precautions have been taken to minimize taxon numbers, it is safe to affirm than the number of fish parasites is at least ten times the number of fish species in coral reefs, for species of similar size or larger than the species in the four families studied; this is a major improvement to our estimate of biodiversity in coral reefs. Our results suggest that extinction of a coral reef fish species

  15. Spatial and temporal variability in the otolith chemistry of the Brazilian snapper Lutjanus alexandrei from estuarine and coastal environments.

    PubMed

    Aschenbrenner, A; Ferreira, B P; Rooker, J R

    2016-07-01

    Otolith chemistry of juvenile and adult individuals of the Brazilian snapper Lutjanus alexandrei was measured to assess the utility of natural markers for investigating individual movements. Individuals were collected over a 3-year period (2010-2012) along the north-eastern coast of Brazil from both estuarine (juvenile to sub-adult stages) and coastal (sub-adult to adult stages) areas. Six elements ((7) Li, (24) Mg, (55) Mn, (59) Co, (88) Sr and (137) Ba) were measured in sectioned otoliths of L. alexandrei using laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS). Edge composition analysis indicated that element:Ca ratios in the otoliths of juvenile and sub-adult L. alexandrei from estuaries were not significantly different among the three consecutive years (2010, 2011 and 2012), suggesting that physicochemical conditions within the nursery area investigated were temporally stable. Similarly, apart from two elements (Ba and Co), element:Ca ratios for larger L. alexandrei inhabiting coastal waters were also similar. In contrast, otolith chemistry of similar sized L. alexandrei from estuarine and coastal areas was significantly different (based on recently accreted material). Otolith Mn:Ca and Ba:Ca were both significantly higher for L. alexandrei collected in estuaries compared to fish from adjacent coastal reefs, while the opposite trend was observed for Sr:Ca. Given the pronounced differences in otolith chemistry between estuarine and coastal areas, element:Ca transects were constructed from the core to margin of the otoliths for adults (age 7+ years) collected on reefs to determine the timing of movement (ontogenetic migration) from estuarine to coastal areas. Based on observed patterns of decline for both Mn:Ca and Ba:Ca, it appears that L. alexandrei begin the move to more coastal habitats (i.e. lower element:Ca ratios) after age 2 years. The patterns observed for this species highlight the importance of conserving connectivity between

  16. New species of Moravecia (Nematoda: Dracunculoidea) from body cavity of marine perciform fish Percophis brasiliensis in Argentina.

    PubMed

    Braicovich, Paola; Moravec, Frantisek; Timi, Juan T

    2007-04-01

    A new species of dracunculoid nematode, Moravecia argentinensis n. sp. (Guyanemidae), is described from the blood vessels and body cavity of Brazilian flathead, Percophis brasiliensis Quoy et Gaimard (Perciformes: Percophidae), from off the coast of Argentina. The new species differs from the only other congeneric species, Moravecia australiensis Ribu et Lester, 2004, mainly in the number (14) of cephalic papillae, the length ratio of the muscular and glandular portions of esophagus (1:1-2), more numerous (3-5 pairs) genital papillae in the male, and the presence of a sclerotized copulatory plate. This is the second known species of Moravecia Ribu et Lester, 2004 and the first species of this genus reported from South American waters.

  17. Myxobolus ophiocarae sp. n. (Myxozoa: Myxosporea: Bivalvulida) infecting the gill of wild goby, Ophiocara porocephala (Perciformes: Gobioidei) in Malaysia.

    PubMed

    Borkhanuddin, Muhammad Hafiz; Cech, Gábor; Mazelan, Suhairi; Shaharom-Harrison, Faizah; Molnár, Kálmán; Székely, Csaba

    2014-01-01

    The authors studied the myxosporean infection of wild gobiid fishes (Perciformes: Gobioidei) in the Merang Estuary of Terengganu, Malaysia, and described Myxobolus ophiocarae sp. n. in Ophiocara porocephala. Several myxosporean plasmodia were found intralamellarly within the gill filaments. The spores differed from those of other Myxobolus species previously recorded on gobiid fishes. They were round in valvular view and lens-shaped in sutural view, and had two equal-sized, pyriform polar capsules with polar filaments having six to seven turns. The spores measured 10.34 × 8.79 × 4.53 μm. The 18S rDNA sequence of M. ophiocarae sp. n., based on a contiguous sequence of 1,789 base pairs, differed from any other Myxobolus spp. in GenBank. Phylogenetic analysis of the 18S rDNA gene revealed that this species showed the closest similarity to Myxobolus nagaraensis, Myxobolus lentisuturalis, and Myxobolus cultus.

  18. A new species of Gobius (Perciformes: Gobiidae) from the Mediterranean Sea and the redescription of Gobius bucchichi.

    PubMed

    Kovačić, M; Šanda, R

    2016-03-01

    A new species of the gobiid genus Gobius (Gobiidae, Perciformes), Gobius incognitus sp. nov. is described from the Mediterranean Sea, and its most morphologically similar species Gobius bucchichi is redescribed. The new species is distinguished from its congeners by: scales in lateral series 51-59; predorsal scales 25-35; opercle scaled in adults with 10-16 scales present; pectoral fin with ray count 18-20 and free tips on upper rays well developed and on the first ray longer than two thirds of the entire ray length; pelvic disc complete and with well-developed anterior membrane without lateral lobes; anterior oculoscapular canal with pore α at rear of orbit; oculoscapular row x(1) not extending forwards to pore β; suborbital row d discontinuous with large gap below suborbital rows 3 and 4; eye diameter 1·08-1·32 in snout length; by pigment rows on cheek and pigmentation on pectoral-fin base.

  19. Effects of dietary protein level on growth and utilization of protein and energy by juvenile mangrove red snapper (Lutjanus argentimaculatus)

    NASA Astrophysics Data System (ADS)

    Ghulam, Abbas; Khalid, Jamil; Rukhsana, Akhtar; Lin, Hong

    2005-01-01

    A feeding trial was conducted in a recirculating water system to investigate the effects of dietary protein levels on growth, feed utilization, hepatosomatic index and liver lipid deposition of juvenile red snapper, Lutjanus argentimaculatus (average initial wet weight 8.0 ± 0.39 g and total length 3.14 ± 0.3 cm). In the experiment, six fishmeal-based diets were formulated to contain various protein levels (20% to 45% in 5% increments), with dietary energy ranging from 2210.7kJ lOOg to 2250.2kJlOOg dry matter. The protein to energy ratios of diets ranged from 8.58 mg protein kJ-1 to 20.03 mg protein kJ-1. Diets were fed for 90d to triplicate groups of fish stocked in 0.128m3 seawater tanks, 25 individuals each. The daily ration of 2% wet body weight was offered to the fish thrice a day. The fish at the end of the study had more than ten-fold (77.0g) increase in weight compared to the initial (8.0g). Fish fed diets of 40% and 45% protein produced significantly (P<0.05) higher weight gain of 77.2g and 76.5g, and specific growth rate (SGR) of 2.65% and 2.62% than those of 67.0 g and 68.3g, and 2.49% and 2.51% of the other diets. The broken-line regression of SGR against dietary protein level yielded an optimum dietary protein requirement of 42.6% (Y=-1.6295 + 0.1114 X 2,P<0.05). Survival remained 100% among groups. Feed conversion ratio decreased from 0.45 for fish fed 20% dietary protein to 0.35 for fish fed 45% dietary protein. Nitrogen intake increased with an increase in dietary protein, which in turn resulted in an increase in nitrogen gain of fish whole body. Fish fed 40% and 45% protein diets showed higher (P<0.05) nitrogen gain (0.27g and 0.26g) than those (0.23g and 025g) fed all other diets. Gross energy intake (GEI) in fish fed 45% protein was lower (600.67kJ) than that (607.97 kJ) of 40% protein diet, though the differences were not statistically significant (P>0.05); GEI ranging from 677.31 kJ to 663.20 kJ at remaining four diets (20% to 35% protein

  20. A new species of Anchistrotos Brian, 1906 (Copepoda: Cyclopoida: Taeniacanthidae) from the filamentous shrimpgoby Myersina filifer (Valenciennes) (Perciformes: Gobiidae) in Korean waters.

    PubMed

    Moon, Seong Yong; Lee, Jeong-Hoon; Kim, Doo Nam

    2015-10-01

    A new species of Anchistrotos Brian, 1906 (Copepoda: Cyclopoida: Taeniacanthidae), parasitic in the branchial cavities of the filamentous shrimpgoby Myersina filifer (Valenciennes) (Perciformes: Gobiidae) from Korea is described. The new species is most closely related to A. tangi Venmathi Maran, Moon & Adday, 2014, but differs from it by the following combination of characters in the adult female: the U-shaped rostrum, the distal margin of the anal somite lacks patches of spinules, the proximal segment of the maxilliped is without seta, and the maxilliped claw is armed with long and small naked setae. This is the tenth species of the genus and a key is provided to distinguish all nominal species.

  1. Trends on the Karyotype Acrocentrization Within Carangidae (Perciformes): A New Phylogenetic Evidence About a Traditional Marine Paradigm.

    PubMed

    Jacobina, Uedson Pereira; Martinez, Pablo Ariel; Torres, Rodrigo Augusto; Souza, Gustavo

    2016-02-01

    Carangidae is a morphologically diverse family of marine fish, characterized by stable karyotypes, predominantly with 2n = 48, composed of acrocentric chromosomes (A). This stability is shared with other families of the order Perciformes, which resulted in the hypothesis that 48A is a plesiomorphic karyotype of the group. We tested this hypothesis in the Carangidae family using comparative phylogenetic methods, investigating the evolution of karyotype characters (including chromosome number, morphology, and number of chromosome arms per karyotype [fundamental number, FN]). Our analyses revealed that 2n = 48 is most likely the ancestral chromosome number for the family. However, an extremely variable number of FNs, always above 48, was observed in basal clades within the family and sister groups. On the other hand, the reduced FN = 48 was consistently observed only in the most derived clades, indicating a tendency for acrocentrization. The number of acrocentric chromosomes apparently was accompanied by a trend of reduction in the genome size (1C-value), suggesting that these changes might be correlated. Our data contradict the marine fish hypothesis that the 2n = 48 acrocentric karyotype is plesiomorphic, at least for Carangidae, and reveal the importance for the correct interpretation of karyotype in a temporal and phylogenetic context.

  2. New species of Cardicola (Digenea: Aporocotylidae) from heart of Atlantic croaker, Micropogonias undulatus (Perciformes: Sciaenidae), of the South Atlantic Bight.

    PubMed

    Bullard, Stephen A; Baker, Tiffany; de Buron, Isaure

    2012-04-01

    Cardicola parvus n. sp. (Digenea: Aporocotylidae) infects the heart of Atlantic croaker, Micropogonias undulatus (Linnaeus, 1766) (Perciformes: Sciaenidae), in the South Atlantic Bight off Cow Island (34°38'49″N, 76°33'41″W, type locality) and Figure Eight Island (34°15'48″N, 77°44'27″W), North Carolina, USA, and off Jacksonville Beach (30°08'23″N, 81°20'52″W), Florida, USA. The new species is most easily differentiated from other members of Cardicola Short, 1953 by the combination of having a minute adult body (≤ 1 mm total length) that is 3.1-4.7× longer than wide, widely dispersed ventral tegumental sensory papillae, ~180 tegumental spine rows per side of body, a spheroid anterior sucker that is apparently aspinous, an esophagus that is 38-39% of the body total length, a male genital pore that is anterior to the ootype, a uterus that transitions from ascending to descending portions posterolaterally to the ovary, and a nearly transverse oviducal seminal receptacle. The new species is the second named aporocotylid from a littoral fish of the South Atlantic Bight and the fifth aporocotylid species reported from fishes of the northwestern Atlantic Ocean.

  3. Complete mitochondrial genome of the giant croaker Nibea japonica (Perciformes, Sciaenidae) and phylogenetic analysis of the Sciaenidae.

    PubMed

    Hu, Zehui; Chai, Xuejun; Wang, Yuebin; Zhu, Yunhai; Zhu, Dongfa

    2016-09-01

    The giant croaker Nibea japonica (Perciformes, Sciaenidae) is an important economic fish distributing in the East China Sea, South China Sea, and Japan southern coast. In this study, the complete mitochondrial genome of N. japonica was firstly determined. It is 16 496 bp-length and consists of 22 tRNA genes, 13 protein-coding genes, two rRNA genes, and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. Phylogenetic analysis revealed that N. japonica, A. amoyensis, and other seven fish first clustered into the Argyrosominae clade. It is consistent with the taxonomic status. Then, the Argyrosominae, Pseudosciaeninae, and Sciaeniae formed the sister group, while the Johniinae became a separate clade, which is inconsistent with the previous phenotypic report. It is suggested that the researches of single gene and taxionomic might lose some significant evolutionary characters. This study will contribute to phyogenetic analysis of the Sciaenidae and the natural resources conservation.

  4. Aporocotyle michaudi n. sp. (Digenea: Aporocotylidae) from the emerald rock cod, Trematomus bernacchii (Teleostei: Perciformes) in Antarctica.

    PubMed

    Santoro, Mario; Cipriani, Paolo; Pankov, Plamen; Lawton, Scott P

    2015-10-01

    Aporocotyle michaudi n. sp. is described from the gill blood vessels of the emerald rock cod Trematomus bernacchii in the Ross Sea, Antarctica. It is distinguished from all other species of Aporocotyle by its body tegument showing single conical spines, spinous buccal capsule, and genital atrium positioned medially; all congeners described to date are characterized by clusters of tegumental spines, unspined buccal capsule and genital atrium located in the lateral part of the body. Aporocotyle michaudi n. sp. clearly differs from A. notothenia (the only other species of Aporocotyle found in a perciform fish) in its shape and arrangement of tegumental spines, buccal capsule features, location of genital atrium, body size, ratio of esophagus/body length, anterior caeca/posterior caeca ratio, number of testes, cirrus sac and ovary size and shape, and host. The new species is easily distinguished from A. argentinensis (the species that most closely resembles A. michaudi) by the shape and arrangement of tegumental spines, buccal capsule features, genital atrium location, left anterior caecum longer than right, esophagus/body length ratio, number of testes, cirrus sac size and shape, host and molecular analyses. Phylogenetic analyses of partial 28S rDNA genetic data showed that sequences representing the new species form a distinct clade with all other sequences for species of Aporocotyle and appear basal within the genus. Aporocotyle michaudi n. sp. represents the only species of genus described in Antarctica. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  5. The complete mitochondrial genome of the Anabas testudineus (Perciformes, Anabantidae) and its comparison with other related fish species.

    PubMed

    Behera, Bijay Kumar; Baisvar, Vishwamitra Singh; Kumari, Kavita; Rout, Ajaya Kumar; Pakrashi, Sudip; Paria, Prasenjet; Rao, A R; Rai, Anil

    2017-03-01

    In the present study, the complete mitochondrial genome sequence of Anabas testudineusis reported using PGM sequencer (Ion Torrent, Life Technologies, La Jolla, CA). The complete mitogenome of climbing perch, A. testudineusis obtained by the de novo sequences assembly of genomic reads using the Torrent Mapping Alignment Program (TMAP), which is 16 603 bp in length. The mitogenome of A. testudineus composed of 13 protein- coding genes, two rRNA, and 22 tRNAs. Here, 20 tRNAs genes showed typical clover leaf model, and D-Loop as the control region along with gene order and organization, being closely similar to Osphronemidae and most of other Perciformes fish mitogenomes of NCBI databases. The mitogenome in the present study has 99% similarity to the complete mitogenome sequence of earlier reported A. testudineus. The phylogenetic analysis of Anabantidae depicted that their mitogenomes are closely related to each other. The complete mitogenome sequence of A. testudineus would be helpful in understanding the population genetics, phylogenetics, and evolution of Anabantidae.

  6. Evidence of birth-and-death evolution of 5S rRNA gene in Channa species (Teleostei, Perciformes).

    PubMed

    Barman, Anindya Sundar; Singh, Mamta; Singh, Rajeev Kumar; Lal, Kuldeep Kumar

    2016-12-01

    In higher eukaryotes, minor rDNA family codes for 5S rRNA that is arranged in tandem arrays and comprises of a highly conserved 120 bp long coding sequence with a variable non-transcribed spacer (NTS). Initially the 5S rDNA repeats are considered to be evolved by the process of concerted evolution. But some recent reports, including teleost fishes suggested that evolution of 5S rDNA repeat does not fit into the concerted evolution model and evolution of 5S rDNA family may be explained by a birth-and-death evolution model. In order to study the mode of evolution of 5S rDNA repeats in Perciformes fish species, nucleotide sequence and molecular organization of five species of genus Channa were analyzed in the present study. Molecular analyses revealed several variants of 5S rDNA repeats (four types of NTS) and networks created by a neighbor net algorithm for each type of sequences (I, II, III and IV) did not show a clear clustering in species specific manner. The stable secondary structure is predicted and upstream and downstream conserved regulatory elements were characterized. Sequence analyses also shown the presence of two putative pseudogenes in Channa marulius. Present study supported that 5S rDNA repeats in genus Channa were evolved under the process of birth-and-death.

  7. Neobenedenia melleni Parasite of Red Snapper, Lutjanus erythropterus, with Regression Statistical Analysis between Fish Length, Temperature, and Parasitic Intensity in Infected Fish, Cultured at Jerejak Island, Penang, Malaysia

    PubMed Central

    Ravi, Rajiv

    2016-01-01

    The fish parasites collected from Lutjanus erythropterus fish species showed a correlation with parasitic intensity, fish size, and temperature, and statistical model summary was produced using SPSS version 20, statistical software. Statistical model summary concluded that among the variables which significantly predict the prevalence of Neobenedenia melleni parasites are fish length and water temperature, both significant at 1% and 5%. Furthermore, the increase in one unit of fish length, holding other variables constant, increases the prevalence of parasite by approximately 1 (0.7≈1) unit. Also, increasing the temperature from 32°C to 33°C will positively increase the number of parasites by approximately 0.32 units, holding other variables constant. The model can be summarized as estimated number of Neobenedenia melleni parasites = 8.2 + 0.7 ⁎ (fish length) + 0.32 ⁎ (water temperature). Next, this study has also shown the DNA sequence and parasitic morphology of Neobenedenia melleni. Nucleotide sequence for 18s ribosomal gene RNA in this study showed 99% similarity with N. melleni EU707804.1 from GenBank. Finally, all the sequence of Neobenedenia melleni in this study was deposited in GenBank with accession numbers of KU843501, KU843502, KU843503, and KU843504. PMID:27190634

  8. Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol)

    PubMed Central

    Noor Aini, Bohari; Siddiquee, Shafiquzzaman; Ampon, Kamaruzaman

    2016-01-01

    Electrochemical biosensors are widely recognized in biosensing devices due to the fact that gives a direct, reliable, and reproducible measurement within a short period. During bio-interaction process and the generation of electrons, it produces electrochemical signals which can be measured using an electrochemical detector. A formaldehyde biosensor was successfully developed by depositing an ionic liquid (IL) (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), gold nanoparticles (AuNPs), and chitosan (CHIT), onto a glassy carbon electrode (GCE). The developed formaldehyde biosensor was analyzed for sensitivity, reproducibility, storage stability, and detection limits. Methylene blue was used as a redox indicator for increasing the electron transfer in the electrochemical cell. The developed biosensor measured the NADH electron from the NAD+ reduction at a potential of 0.4 V. Under optimal conditions, the differential pulse voltammetry (DPV) method detected a wider linear range of formaldehyde concentrations from 0.01 to 10 ppm within 5 s, with a detection limit of 0.1 ppm. The proposed method was successfully detected with the presence of formalin in fish samples, Lutjanus malabaricus and Thunnus Tonggol. The proposed method is a simple, rapid, and highly accurate, compared to the existing technique. PMID:27376338

  9. Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol).

    PubMed

    Noor Aini, Bohari; Siddiquee, Shafiquzzaman; Ampon, Kamaruzaman

    2016-06-30

    Electrochemical biosensors are widely recognized in biosensing devices due to the fact that gives a direct, reliable, and reproducible measurement within a short period. During bio-interaction process and the generation of electrons, it produces electrochemical signals which can be measured using an electrochemical detector. A formaldehyde biosensor was successfully developed by depositing an ionic liquid (IL) (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), gold nanoparticles (AuNPs), and chitosan (CHIT), onto a glassy carbon electrode (GCE). The developed formaldehyde biosensor was analyzed for sensitivity, reproducibility, storage stability, and detection limits. Methylene blue was used as a redox indicator for increasing the electron transfer in the electrochemical cell. The developed biosensor measured the NADH electron from the NAD⁺ reduction at a potential of 0.4 V. Under optimal conditions, the differential pulse voltammetry (DPV) method detected a wider linear range of formaldehyde concentrations from 0.01 to 10 ppm within 5 s, with a detection limit of 0.1 ppm. The proposed method was successfully detected with the presence of formalin in fish samples, Lutjanus malabaricus and Thunnus Tonggol. The proposed method is a simple, rapid, and highly accurate, compared to the existing technique.

  10. Two new species of Aristocleidus (Monogenea) from the gills of the Mexican mojarra Eugerres mexicanus (Perciformes, Gerreidae) from southwestern Mexico.

    PubMed

    Mendoza-Franco, Edgar F; Osorio, Marina Tapia; Caspeta-Mandujano, Juan Manuel

    2015-01-01

    Aristocleidus mexicanus n. sp. and Aristocleidus lacantuni n. sp. are described from the gills of the Mexican mojarra Eugerres mexicanus (Gerreidae, Perciformes) from the Rio Lacantún basin, Chiapas State, Mexico. These new species differ from previously described congeneric species in the characteristics of several structures, including: (a) ventral anchors, with differences in length (i.e. 46-50 µm in A. mexicanus vs. 38-43 µm, 34-37 µm, and 26-33 µm in Aristocleidus hastatus Mueller, 1936, Aristocleidus sp. of Mendoza-Franco, Violante-González & Roche 2009, and Aristocleidus lamothei Kritsky & Mendoza-Franco, 2008, respectively) and shape (i.e. slightly angular union of elongate arcing shaft and point in A. mexicanus vs. point and shaft united at a conspicuous angular bend in A. hastatus and Aristocleidus sp., and evenly curved shaft and point in A. lamothei); (b) male copulatory organ, i.e. a coiled tube with less than one ring in A. mexicanus and A. lacantuni (vs. a coiled tube of about 1½ in Aristocleidus sp.); (c) distal end of the accessory piece (ornate in A. mexicanus vs. distally flattened and trifid in A. hastatus and A. lamothei, respectively); (d) vaginal tube (moderately long in A. mexicanus vs. short in A. lamothei and looping in Aristocleidus sp.); and (e) ventral bar (anteromedial process with terminal horn-like ornamentation in A. lacantuni vs. ornamentation absent in the other species). This study reports for the first time species of Aristocleidus from freshwater environments in Mexico.

  11. Functional morphology of the luminescence system of Siphamia versicolor (Perciformes: Apogonidae), a bacterially luminous coral reef fish.

    PubMed

    Dunlap, Paul V; Nakamura, Masaru

    2011-08-01

    Previous studies of the luminescence system of Siphamia versicolor (Perciformes: Apogonidae) identified a ventral light organ, reflector, lens, duct, and a ventral diffuser extending from the throat to the caudal peduncle. The control and function of luminescence in this and other species of Siphamia, however, have not been defined. Morphological examination of fresh and preserved specimens identified additional components of the luminescence system involved in control and ventral emission of luminescence, including a retractable shutter over the ventral face of the light organ, contiguity of the ventral diffuser from the caudal peduncle to near the chin, and transparency of the bones and other tissues of the lower jaw. The shutter halves retract laterally, allowing the ventral release of light, and relax medially, blocking ventral light emission; topical application of norepinephrine to the exposed light organ resulted in retraction of the shutter halves, which suggests that operation of the shutter is under neuromuscular control. The extension of the diffuser to near the chin and transparency of the lower jaw allow a uniform emission of luminescence over the entire ventrum of the fish. The live aquarium-held fish were found to readily and consistently display ventral luminescence. At twilight, the fish left the protective association with their longspine sea urchin, Diadema setosum, and began to emit ventral luminescence and to feed on zooplankton. Ventral luminescence illuminated a zone below and around the fish, which typically swam close to the substrate. Shortly after complete darkness, the fish stopped feeding and emitting luminescence. These observations suggest that S. versicolor uses ventral luminescence to attract and feed on zooplankton from the reef benthos at twilight. Ventral luminescence may allow S. versicolor to exploit for feeding the gap at twilight in the presence of potential predators as the reef transitions from diurnally active to

  12. Two new species of Aristocleidus (Monogenea) from the gills of the Mexican mojarra Eugerres mexicanus (Perciformes, Gerreidae) from southwestern Mexico

    PubMed Central

    Mendoza-Franco, Edgar F.; Osorio, Marina Tapia; Caspeta-Mandujano, Juan Manuel

    2015-01-01

    Aristocleidus mexicanus n. sp. and Aristocleidus lacantuni n. sp. are described from the gills of the Mexican mojarra Eugerres mexicanus (Gerreidae, Perciformes) from the Rio Lacantún basin, Chiapas State, Mexico. These new species differ from previously described congeneric species in the characteristics of several structures, including: (a) ventral anchors, with differences in length (i.e. 46–50 µm in A. mexicanus vs. 38–43 µm, 34–37 µm, and 26–33 µm in Aristocleidus hastatus Mueller, 1936, Aristocleidus sp. of Mendoza-Franco, Violante-González & Roche 2009, and Aristocleidus lamothei Kritsky & Mendoza-Franco, 2008, respectively) and shape (i.e. slightly angular union of elongate arcing shaft and point in A. mexicanus vs. point and shaft united at a conspicuous angular bend in A. hastatus and Aristocleidus sp., and evenly curved shaft and point in A. lamothei); (b) male copulatory organ, i.e. a coiled tube with less than one ring in A. mexicanus and A. lacantuni (vs. a coiled tube of about 1½ in Aristocleidus sp.); (c) distal end of the accessory piece (ornate in A. mexicanus vs. distally flattened and trifid in A. hastatus and A. lamothei, respectively); (d) vaginal tube (moderately long in A. mexicanus vs. short in A. lamothei and looping in Aristocleidus sp.); and (e) ventral bar (anteromedial process with terminal horn-like ornamentation in A. lacantuni vs. ornamentation absent in the other species). This study reports for the first time species of Aristocleidus from freshwater environments in Mexico. PMID:26605987

  13. [Analysis of diet composition and overlap in four species of the genus Diplectrum (Perciformes: Serranidae) in the Mexican Central Pacific].

    PubMed

    Aguilar Palomino, Bernabé; González Sansón, Gaspar

    2010-12-01

    Analysis of diet composition and overlap in four species of the genus Diplectrum (Perciformes: Serranidae) in the Mexican Central Pacific. The information of trophic interactions among species is essential to understand ecosystem function. To assess this in four Diplectrum species, we analyzed the stomach contents of 397 individuals caught using shrimp trawling nets off the coasts of Jalisco and Colima, Mexico. Main food component of D. eumelum were fish of the Order Pleuronectiformes, followed by shrimps (Metapenaeopsis spp). D. euryplectrum feeds mainly on crustaceans, with stomatopods (Squilla mantoidea) as main food items followed by shrimps and brachyurans. Fish and polychaetes had a low representation in the diet of this species. D. labarum feeds mainly on crustaceans, mollusks and fish. The stomatopod Eurysquilla veleronis contributed to with a high diet percentage by weight, followed by shrimps (mostly Solenoceraflorae) and brachyurans, as well as the squid Lolliguncula diomedae. Fishes (Ophidion spp.) were also an important component of the diet of this species. The most common preys of D. rostrum were crustaceans, mollusks, polychaetes and fish. The squid Lolliguncula diomedae and fishes of genus Ophidion were also well represented in the diet. Diet breadth index yielded significant differences between species. The number of diet items varied from 16 in D. euryplectrum to 6 in D. eumelum. The average value of overlap (0.247) was not significantly different (p = 0.118) from the expected one for a null model (0.174). The observed variance of the overlap index (0.071) was significantly higher (V = 0.025, p = 0.0004) than the value expected for a null model.

  14. Dietary administration of microalgae alone or supplemented with Lactobacillus sakei affects immune response and intestinal morphology of Pacific red snapper (Lutjanus peru).

    PubMed

    Reyes-Becerril, Martha; Angulo, Carlos; Estrada, Norma; Murillo, Ylenia; Ascencio-Valle, Felipe

    2014-09-01

    The aim of this study was to evaluate the effect of dietary microalgae Navicula sp single or in combination with Lactobacillus sakei on growth performance, humoral immune parameters and intestinal morphology in Pacific red snapper, Lutjanus peru. The experimental fish were grouped into four treatment diets which were a control diet (commercial diet, Control), silage microalgae Navicula sp plus L. sakei (10(6) CFU g(-1), Navicula + L. sakei), lyophilized microalgae (Navicula) and L. sakei (10(6) CFU g(-1), L. sakei). The blood and intestine samples were collected on week 4 and 8. The weight gain showed an additive effect of Navicula + L. sakei at 8 weeks of treatment compared with fish fed control diet. Overall, physiological parameters such as total protein and hemoglobin were increased in fish fed with Navicula and L. sakei diets at 4 and 8 weeks of feeding assay, respectively. There was a significant improvement in immune parameters, principally in myeloperoxidase, lysozyme, total antiproteases activities and IgM in fish fed with Navicula + L. sakei and L. sakei diets at 4 or 8 weeks of treatments. Serum antioxidant capabilities revealed significant increase in phosphatase alkaline, esterase, protease, superoxide dismutase and catalase in groups which received diet supplemented with Navicula + L. sakei and L. sakei diets. Finally, light microscopy observations revealed no effect of experimental diets on microvilli height. Curiously, the presence of vacuoles inside the enterocytes was significant higher in the intestine of L. sakei group after four or six weeks of feeding. Elevated intraepithelial leucocyte levels and melanomacrophages centers were observed in fish fed Navicula or control diets at any time of the experiment. To conclude, the results of the present study demonstrate that the fish that were fed with Navicula + L. sakei or L. sakei diets yielded significantly better immune status and antioxidant capabilities. Copyright © 2014 Elsevier Ltd. All

  15. Severe glomerular disease in juvenile grey snapper Lutjanus griseus L. in the Gulf of Mexico caused by the myxozoan Sphaerospora motemarini n. sp.

    PubMed

    Holzer, Astrid S; Pecková, Hanka; Patra, Sneha; Brennan, Nathan P; Yanes-Roca, Carlos; Main, Kevan L

    2013-12-01

    In the eastern Gulf of Mexico, off the coast of Florida, grey snapper, Lutjanus griseus was found to be infected with the myxozoan parasite Sphaerospora motemarini n. sp., with high prevalence (83%) and intensity of infection occuring in age-0 fish, and with parasite levels decreasing with age (age-1 snapper 40%; age-2 snapper 0%). The morphological, molecular and phylogenetic characterisation of the myxozoan showed that it is a member of the typically marine, polysporoplasmid Sphaerospora spp. which form a subclade within the Sphaerospora sensu stricto clade of myxozoans, which is characterised by large expansion segments in their SSU rDNA sequences. Presporogonic stages of S. motemarini n. sp. were detected in the blood, using PCR. Pseudoplasmodia and spores were found to develop in the renal corpuscles of the host, causing their massive expansion. Macroscopic and histopathological changes were observed in age-0 fish and show that S. motemarini n. sp. causes severe glomerulonephritis in L. griseus leading to a compromised host condition, which makes it more susceptible to stress (catch-and-release, predators, water quality) and can result in mortalities. These results are discussed in relation to the exploitation of grey snapper populations by commercial and recreational fisheries and with the observed increased mortalities with temperature along the coast of Florida. In the future, we would like to determine prevalence and intensity of infection with S. motemarini n. sp. in juvenile L. griseus in different areas of the Gulf of Mexico in order to be able to estimate the temperature dependence of S. motemarini n. sp. proliferation and to be able to predict its distribution and severity during climatic changes in the Gulf.

  16. Effects of marine silages enriched with Lactobacillus sakei 5-4 on haemato-immunological and growth response in Pacific red snapper (Lutjanus peru) exposed to Aeromonas veronii.

    PubMed

    Reyes-Becerril, Martha; Ascencio-Valle, Felipe; Macias, María Esther; Maldonado, Minerva; Rojas, Maurilia; Esteban, María Ángeles

    2012-10-01

    Combined effects of marine silages enriched with Lactobacillus sakei 5-4 were evaluated on growth performance, immune activity and disease resistance of Pacific red snapper (Lutjanus peru) against Aeromonas veronii infection. The experimental fish were divided into three groups which were fed with each one of the following diets: silage-probiotic-free diet (control, C group), Pacific creole-fish silage diet supplemented with live L. sakei (10(6) CFU g(-1)) (FSLact group) and Humboldt squid silage diet supplemented with live L. sakei (10(6) CFU g(-1)) (SSLact group) for 6 weeks. After 6 weeks, fish were immunocompromised with pathogenic A. veronii and spleen and liver samples were processed for histopathological studies. Generally, the results showed enhanced growth performance in fish fed the diet containing SSLact at 6 and 7 weeks compared with fish fed control diet. Addition of SSLact had an increase in plasmatic protein at week 6 and post-challenge. Hemoglobin concentration increased after challenge in fish fed with SSLact compared to control group. At week 6 and post-challenge the results indicated that, the fish groups which received diet supplemented with SSLact revealed significant increase in humoral immune parameters. Histologically, fish fed C diets showed marked fatty degeneration and great activation of melanomacrophage centers compare with SSLact and FSLact groups. These results support the idea that the marine silages with squid as protein source enriched or combined with L. sakei 5-4 increases the body weight and stimulates the physiological and humoral immune parameters in Pacific red snapper infected with A. veronii.

  17. Effects of temperature and diet on length-weight relationship and condition factor of the juvenile Malabar blood snapper (Lutjanus malabaricus Bloch & Schneider, 1801)*

    PubMed Central

    Mazumder, Sabuj Kanti; Das, Simon Kumar; Bakar, Yosni; Ghaffar, Mazlan Abd.

    2016-01-01

    In this study we aimed to analyze the effects of water temperature and diet on the length-weight relationship and condition of juvenile Malabar blood snapper Lutjanus malabaricus over a 30-d experimental period. The experiment was conducted in the laboratory using a flow-through-sea-water system. The fish were subjected to four different temperatures (22, 26, 30, and 34 °C) and two diets (commercial pellet and natural shrimp). Fish were fed twice daily. L. malabaricus exhibited negative allometric growth (b<3) at the beginning of the experiment (Day 0) at all temperatures and both diets except for 22 °C fed with shrimp, which showed isometric growth (b=3). Conversely, at the end of the experiment (Day 30) fish showed isometric growth (b=3) at 30 °C fed with the pellet diet, indicating that the shape of the fish did not change with increasing weight and length, and a positive allometric growth (b>3) at 30 °C fed with shrimp diet, which indicated that fish weight increases faster than their length. The rest of the temperatures represented negative allometric growth (b<3) on both diet, meaning that fish became lighter with increasing size. The condition factors in the initial and final measurements were greater than 1, indicating the state of health of the fish, except for those fed on a pellet diet at 34 °C. However, the best condition was obtained at 30 °C on both diets. Nevertheless, diets did not have a significant effect on growth and condition of juvenile L. malabaricus. The data obtained from this study suggested culturing L. malabaricus at 30 °C and feeding on the pellet or shrimp diet, which will optimize the overall production and condition of this commercially important fish species. PMID:27487803

  18. Severe glomerular disease in juvenile grey snapper Lutjanus griseus L. in the Gulf of Mexico caused by the myxozoan Sphaerospora motemarini n. sp.

    PubMed Central

    Holzer, Astrid S.; Pecková, Hanka; Patra, Sneha; Brennan, Nathan P.; Yanes-Roca, Carlos; Main, Kevan L.

    2013-01-01

    In the eastern Gulf of Mexico, off the coast of Florida, grey snapper, Lutjanus griseus was found to be infected with the myxozoan parasite Sphaerospora motemarini n. sp., with high prevalence (83%) and intensity of infection occuring in age-0 fish, and with parasite levels decreasing with age (age-1 snapper 40%; age-2 snapper 0%). The morphological, molecular and phylogenetic characterisation of the myxozoan showed that it is a member of the typically marine, polysporoplasmid Sphaerospora spp. which form a subclade within the Sphaerospora sensu stricto clade of myxozoans, which is characterised by large expansion segments in their SSU rDNA sequences. Presporogonic stages of S. motemarini n. sp. were detected in the blood, using PCR. Pseudoplasmodia and spores were found to develop in the renal corpuscles of the host, causing their massive expansion. Macroscopic and histopathological changes were observed in age-0 fish and show that S. motemarini n. sp. causes severe glomerulonephritis in L. griseus leading to a compromised host condition, which makes it more susceptible to stress (catch-and-release, predators, water quality) and can result in mortalities. These results are discussed in relation to the exploitation of grey snapper populations by commercial and recreational fisheries and with the observed increased mortalities with temperature along the coast of Florida. In the future, we would like to determine prevalence and intensity of infection with S. motemarini n. sp. in juvenile L. griseus in different areas of the Gulf of Mexico in order to be able to estimate the temperature dependence of S. motemarini n. sp. proliferation and to be able to predict its distribution and severity during climatic changes in the Gulf. PMID:24533325

  19. Contrasting patterns of reef utilization and recruitment of coral trout ( Plectropomus leopardus) and snapper ( Lutjanus carponotatus) at One Tree Island, southern Great Barrier Reef

    NASA Astrophysics Data System (ADS)

    Kingsford, M. J.

    2009-03-01

    Patterns of abundance, age structure and recruitment of coral trout ( Plectropomus leopardus) and snapper ( Lutjanus carponotatus) were described in different environments, which varied in benthic cover, in a 12-yr study at One Tree Island. It was hypothesized that both taxa would show strong preferences to different environments and benthic cover and that patterns would be consistent through time. Plectropomus leopardus were abundant on the reef slope and seaward edge of the lagoon, where live coral cover was high, and recruitment was generally low, in all environments. The population was sustained by a trickle of recruits, and total abundance varied little after 10 to 25 yr of protection in a no-take area, suggesting P. leopardus had reached an environment-related carrying capacity. Protogynous P. leopardus recruited to shallow environments at sites with 20% or more hard live coral and age data indicated the abundance of fish on the reef slope was from redistribution. Most recruits of gonochoristic L. carponotatus (<150 mm Standard length, SL) were found in the lagoonal environments, and adults were rare on the reef slope. Abundance of recruit L. carponotatus and P. leopardus did not correlate with percent cover of live and soft coral within environments. Recruits of L. carponotatus were usually rare in all lagoonal environments, but in 2003, many recruits (80 to 120 mm SL) were found in lagoonal environments with low and high hard live coral cover. A substantial proportion of the population (age max 18 yr) was from strong recruitment events. In 2003 and 2004, total abundance of L. carponotatus was supported by 1 year class 51.7 and 41% respectively. The utilization of environments and types of substrata varied among taxa and in some cases among life-history stages. There was also temporal variation in the importance of some environments (e.g. Lagoon Centre).

  20. Nutrition and feeding research in the spotted rose snapper (Lutjanus guttatus) and bullseye puffer (Sphoeroides annulatus), new species for marine aquaculture.

    PubMed

    García-Ortega, Armando

    2009-03-01

    The spotted rose snapper (Lutjanus guttatus) and bullseye puffer (Sphoeroides annulatus) are fish species from the tropical Eastern Pacific for which controlled production of larvae and juveniles has been accomplished in recent years. Diverse topics relating to their biology and aquaculture production are currently under study, in particular the nutrition and feeding aspects required to formulate practical feeds and rearing protocols. Improvements in larval growth and survival are possible by feeding live food organisms with natural or enhanced essential fatty acids content and highly digestible artificial microdiets. The ontogeny of the digestive tract and the expression and activity of digestive enzymes have been described for S. annulatus larvae. The effect of various protein and lipid levels on growth and feed utilization has been studied in juvenile and on-growing fish. Both species have carnivorous feeding habits and require high levels of protein in their diets, from 40% to 45% (dry weight) in spotted rose snapper and above 50% in bullseye puffer, with the younger stages requiring the highest protein levels. Encouraging results have been obtained in feeding experiments with different sources of dietary protein from animal and plant origin to evaluate their suitability as feed ingredients in practical diets. Optimization of fish culture practices through feeding management has also been investigated. Trials with various fish densities and feeding frequencies in intensive culture systems are providing information to improve feed utilization and growth in on-growing fish. Further research is underway to evaluate factors in broodstock nutrition which have an impact on egg and larval quality, and into the use of various commercially available oil sources in on-growing diets. In this paper, the results on nutrition and feeding research with both species are reviewed and research needs to support their commercial production in the region are discussed.

  1. Ontogeny changes and weaning effects in gene expression patterns of digestive enzymes and regulatory digestive factors in spotted rose snapper (Lutjanus guttatus) larvae.

    PubMed

    Moguel-Hernández, I; Peña, R; Andree, K B; Tovar-Ramirez, D; Bonacic, K; Dumas, S; Gisbert, E

    2016-10-01

    The study of digestive physiology is an important issue in species that have been introduced in aquaculture like the spotted rose snapper (Lutjanus guttatus). The aims of this study were to describe the expression of digestive enzymes (trypsinogen, chymotrypsinogen, α-amylase, lipoprotein lipase, phospholipase A and pepsinogen) and their relation with orexigenic (neuropeptide Y, NPY) and anorexigenic (cholecystokinin, CCK) factors during the larval development and to evaluate the effect of weaning in their expression. The results showed that the transcripts of all the assayed digestive enzymes, with the exception of pepsinogen, and NPY and CCK were already present in L. guttatus from the hatching stage. The expression of all the enzymes was low during the yolk-sac stage (0-2 days after hatching, DAH), whereas after the onset of exogenous feeding at 2 DAH, their expression increased and fluctuated throughout larval development, which followed a similar pattern as in other marine fish species and reflected changes in different types of food items and the progressive maturation of the digestive system. On the other hand, weaning of L. guttatus larvae from live prey onto a microdiet between 25 and 35 DAH significantly affected the relative expression of most pancreatic digestive enzymes during the first weaning days, whereas chymotrypsinogen 2 and lipoprotein lipase remained stable during this period. At the end of co-feeding, larvae showed similar levels of gene expression regardless of the diet (live prey vs. microdiet), which indicated that larvae of L. guttatus were able to adapt their digestive capacities to the microdiet. In contrast, feeding L. guttatus larvae with live feed or microdiet did not affect the expression of CCK and NPY. The relevance of these findings with regard to current larval rearing procedures of L. guttatus is discussed.

  2. Effects of temperature and diet on length-weight relationship and condition factor of the juvenile Malabar blood snapper (Lutjanus malabaricus Bloch & Schneider, 1801).

    PubMed

    Mazumder, Sabuj Kanti; Das, Simon Kumar; Bakar, Yosni; Ghaffar, Mazlan Abd

    2016-08-01

    In this study we aimed to analyze the effects of water temperature and diet on the length-weight relationship and condition of juvenile Malabar blood snapper Lutjanus malabaricus over a 30-d experimental period. The experiment was conducted in the laboratory using a flow-through-sea-water system. The fish were subjected to four different temperatures (22, 26, 30, and 34 °C) and two diets (commercial pellet and natural shrimp). Fish were fed twice daily. L. malabaricus exhibited negative allometric growth (b<3) at the beginning of the experiment (Day 0) at all temperatures and both diets except for 22 °C fed with shrimp, which showed isometric growth (b=3). Conversely, at the end of the experiment (Day 30) fish showed isometric growth (b=3) at 30 °C fed with the pellet diet, indicating that the shape of the fish did not change with increasing weight and length, and a positive allometric growth (b>3) at 30 °C fed with shrimp diet, which indicated that fish weight increases faster than their length. The rest of the temperatures represented negative allometric growth (b<3) on both diet, meaning that fish became lighter with increasing size. The condition factors in the initial and final measurements were greater than 1, indicating the state of health of the fish, except for those fed on a pellet diet at 34 °C. However, the best condition was obtained at 30 °C on both diets. Nevertheless, diets did not have a significant effect on growth and condition of juvenile L. malabaricus. The data obtained from this study suggested culturing L. malabaricus at 30 °C and feeding on the pellet or shrimp diet, which will optimize the overall production and condition of this commercially important fish species.

  3. Brain and sense organ anatomy and histology of the Falkland Islands mullet, Eleginops maclovinus (Eleginopidae), the sister group of the Antarctic notothenioid fishes (Perciformes: Notothenioidei).

    PubMed

    Eastman, Joseph T; Lannoo, Michael J

    2008-01-01

    The perciform notothenioid fish Eleginops maclovinus, representing the monotypic family Eleginopidae, has a non-Antarctic distribution in the Falkland Islands and southern South America. It is the sister group of the five families and 103 species of Antarctic notothenioids that dominate the cold shelf waters of Antarctica. Eleginops is the ideal subject for documenting the ancestral morphology of nervous and sensory systems that have not had historical exposure to the unusual Antarctic thermal and light regimes, and for comparing these systems with those of the phyletically derived Antarctic species. We present a detailed description of the brain and cranial nerves of Eleginops and ask how does the neural and sensory morphology of this non-Antarctic notothenioid differ from that seen in the phyletically derived Antarctic notothenioids? The brain of Eleginops is similar to those of visually oriented temperate and tropical perciforms. The tectum is smaller but it has well-developed olfactory and mechanoreceptive lateral line areas and a large, caudally projecting corpus cerebellum. Eye diameter is about twofold smaller in Eleginops than in many Antarctic species. Eleginops has a duplex (rod and cone) retina with single and occasional twin cones conspicuous centrally. Ocular vascular structures include a large choroid rete mirabile and a small lentiform body; a falciform process and hyaloid arteries are absent. The olfactory rosette is oval with 50-55 lamellae, a large number for notothenioids. The inconspicuous bony canals of the cephalic lateral line system are simple with membranous secondary branches that lack neuromasts. In Antarctic species, the corpus cerebellum is the most variable brain region, ranging in size from large and caudally projecting to small and round. "Stalked" brains showing reduction in the size of the telencephalon, tectum, and corpus cerebellum are present in the deep-living artedidraconid Dolloidraco longedorsalis and in most of the deep

  4. Monorchiid trematodes of the painted sweetlips, Diagramma labiosum (Perciformes: Haemulidae), from the southern Great Barrier Reef, including a new genus and three new species.

    PubMed

    Searle, Emily L; Cutmore, Scott C; Cribb, Thomas H

    2014-07-01

    Five monorchiid species are reported from Diagramma labiosum Macleay (Perciformes: Haemulidae) collected from Heron Island on the southern Great Barrier Reef (GBR): two described species, Helicometroides longicollis Yamaguti, 1934 and Diplomonorchis kureh Machida, 2005 and three new species, including one new genus, Asymmetrostoma heronensis n. g., n. sp., Lasiotocus arrhichostoma n. sp. and Proctotrema addisoni n. sp. Helicometroides longicollis and D. kureh were previously reported from the closely related species Diagramma pictum (Thunberg) from Japan. Two further monorchiid species known from D. pictum, Genolopa plectorhynchi (Yamaguti, 1934) and Paraproctotrema fusiforme Yamaguti, 1934, appear to be absent from the southern Great Barrier Reef. Previous reports of two other monorchiids from D. labiosum from the GBR, Paramonorcheides pseudocaranxi Dove & Cribb, 1998 and Helicometroides vitellosus (Durio & Manter, 1968), are shown to have been made in error. The high richness of monorchiids and other trematode families in D. labiosum is consistent with that seen in other haemulids elsewhere.

  5. Morphological characterisation and phylogenetic relationships of Zschokkella candia n. sp. from the gall-bladder of Sparisoma cretense (L.) (Perciformes: Scaridae) in the Sea of Crete off Greece.

    PubMed

    Kalatzis, Panos G; Kokkari, Constantina; Katharios, Pantelis

    2015-11-01

    A new myxosporean parasite, Zschokkella candia n. sp., from the gall-bladder of the wild parrotfish Sparisoma cretense (L.) (Perciformes: Scaridae) is described based on light and scanning electron microscopy. Mature spores are elliptical, with mean dimensions 11.2 ± 0.5 × 7.8 ± 0.1 μm and possess spherical polar capsules with mean diameter of 2.3 ± 0.3 μm. The new species is differentiated from other similar species of the genus based on spore morphology, its coelozoic life-style and molecular data. The phylogenetic tree constructed using maximum likelihood analysis of small subunit (SSU) rDNA sequence data complements the characterisation of Zschokkella candia n. sp. by defining its phylogenetic position among the species of Zschokkella Auerbach, 1909 sequenced to date. The phylogenetic analysis supports the existing knowledge on the complicated polyphyletic relationships among the members of the genus Zschokkella.

  6. Morphological and molecular study of Longicollum pagrosomi Yamaguti, 1935 (Acanthocephala: Pomphorhynchidae) from the barred knifejaw Oplegnathus fasciatus (Temminck & Schlegel) (Perciformes: Oplegnathidae) in the East China Sea.

    PubMed

    Li, Liang; Yang, Yue; Zhang, Lu-Ping

    2017-02-01

    Longicollum pagrosomi Yamaguti, 1935 (Acanthocephala: Pomphorhynchidae) collected from the barred knifejaw Oplegnathus fasciatus (Temminck & Schlegel) (Perciformes: Oplegnathidae) in the East China Sea (off Zhoushan Islands) was studied using light and scanning electron microscopy. The SEM observations revealed for the first time the presence of about 28 well-developed sensory papillae arranged in a circle on the copulatory bursa. In addition, L. pagrosomi was characterised using molecular methods by sequencing of the internal transcribed spacer (ITS) of the ribosomal DNA based on the newly collected material. Longicollum pagrosomi is the first species of the genus with the ITS region sequenced for the purpose of species identification. These new morphological and molecular data contributed to a reliable and accurate specific identification and differentiation of species.

  7. Kinetic determination of vitellogenin induction in the epidermis of cyprinid and perciform fishes: Evaluation of sensitive enzyme-linked immunosorbent assays.

    PubMed

    Allner, Bernhard; Hennies, Mark; Lerche, Cristiano F; Schmidt, Thomas; Schneider, Klaus; Willner, Marco; Stahlschmidt-Allner, Petra

    2016-12-01

    Induction of vitellogenin (VTG) in male and immature fish is a standardized endpoint in endocrine-disruption testing. To establish a nondestructive swab sampling method, VTG induction in the epidermis of Cypriniformes and Perciformes species was investigated. Both VTG and estrogen receptor genes are expressed in epidermal cells. Immunoaffinity and mass fingerprint analyses show induction of identical VTG peptides in liver and epidermis. Induction of VTG by estradiol (E2) and bisphenol A (BPA) in the epidermis was quantified with homolog enzyme-linked immunosorbent assays. Initial values in juveniles and males were below 1 ng VTG/mL extraction buffer. Exposure to E2 led to values between 200 ng/mL and 4600 ng/mL in cyprinids and between 10 ng/mL and 81 ng/mL in perciforms. Exposure to BPA increased VTG amounts to 250 ng/mL in fathead minnows, 1360 ng/mL in goldfish, 100 ng/mL in zebrafish, and 12 ng/mL in bluegills. Serum VTG contents demonstrated a similar dose-response pattern in the epidermis and the blood. These results show that VTG induction may be reliably assessed in the skin mucus of fishes, demonstrating the suitability of this biological sample for investigating estrogenic activity in compliance with Organisation for Economic Co-operation and Development standard protocols. This broadens the perspectives in toxicological screening and environmental monitoring, reducing the number of tested animals and minimizing harmful effects for animals, allowing for follow-up of individual induction profiles. Environ Toxicol Chem 2016;35:2916-2930. © 2016 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC.

  8. Three members of Opisthomonorcheides Parukhin, 1966 (Digenea: Monorchiidae) from carangid fishes (Perciformes) from Indonesia, with a review of the genus.

    PubMed

    Bray, Rodney A; Palm, Harry W; Cutmore, Scott C; Cribb, Thomas H

    2017-03-23

    Three species of Opisthomonorcheides Parukhin, 1966 are reported for the first time from Indonesian waters: O. pampi (Wang, 1982) Liu, Peng, Gao, Fu, Wu, Lu, Gao & Xiao, 2010 and O. ovacutus (Mamaev, 1970) Machida, 2011 from Parastromateus niger (Bloch), and O. decapteri Parukhin, 1966 from Atule mate (Cuvier). Both O. pampi and O. ovacutus can now be considered widespread in the Indo-Pacific region, with earlier records of these species being from Fujian Province, China and Penang, Malaysia, respectively. We redescribe O. decapteri from one of its original hosts, Atule mate, off New Caledonia, and report this species from Jakarta Bay, Indonesia, extending its range throughout the Indian Ocean into the south-western Pacific. All three species possess a genital atrium that is long, sometimes very long, and a genital pore that is located in the forebody. This validates the interpretation that the original description was erroneous in reporting the genital pore in the hindbody, well posterior to the ventral sucker. These observations verify the synonymy of Retractomonorchis Madhavi, 1977 with Opisthomonorcheides. A major discrepancy between the species of Opisthomonorcheides is that some are described with the uterus entering the terminal organ laterally and some with it entering terminally; this feature needs further analysis. Based on the length of the genital atrium and the posterior extent of the vitellarium, the 27 species of Opisthomonorcheides considered valid can be divided into four groups. Among the 53 host records analysed, the families Carangidae (53% of records), Stromateidae (17%) and Serranidae (5.7%) are the most common; the reports are overwhelmingly from members of the Perciformes (91%), with further records in the Clupeiformes (5.7%), Gadiformes (1.9%) and Pleuronectiformes (1.9%). Two fish genera (Parastromateus Bleeker and Pampus Bonaparte) dominate the recorded hosts, with the black pomfret Parastromateus niger harbouring six species, the silver

  9. A New Genus and Two New Species of Proteocephalidean Tapeworms (Cestoda) from Cichlid Fish (Perciformes: Cichlidae) in the Neotropics.

    PubMed

    de Chambrier, Alain; Pinacho-Pinacho, Carlos Daniel; Hernández-Orts, Jesus Servando; Scholz, Tomáš

    2017-02-01

    Cichlidocestus n. gen. is proposed to accommodate 2 new species of proteocephalidean cestodes, Cichlidocestus gillesi n. sp. from Cichlasoma amazonarum in Peru (type species) and Cichlidocestus janikae n. sp. from Hypsophrys nicaraguensis (all Perciformes: Cichlidae) in Costa Rica. The new genus is unique among all but 1 proteocephalidean genera in the position of the ovary that occupies the middle and posterior thirds of the median region of proglottids (vs. the ovary in the posterior third of proglottids near their posterior margin in all but 1 remaining taxa). In addition, Cichlidocestus is typified by the presence of a voluminous, spherical, internal seminal vesicle, several pairs of ventral excretory canals in the medulla, a pyramidal, quadrilobed scolex with an apical muscular sucker, and the posterior extent of the testes that may reach almost to the posterior margin of proglottids. The new genus shares the position of the ovary and its extension with Sciadocephalus (also a parasite of cichlids in the Neotropics as the new taxon); in all remaining proteocephalideans the ovary occupies the posterior third only. Sciadocephalus differs from Cichlidocestus by a different morphology of the scolex, which possesses an umbrella-like metascolex that is markedly wider than the strobila, the number of ventral osmoregulatory canals, and development of the uterus, which forms capsule-like formations filled with eggs in Sciadocephalus megalodiscus (vs. simple lateral diverticula in Cichlidocestus spp.). Both new species of Cichlidocestus can be easily distinguished from one another by the anterior extent of the poral vitelline follicles (anterior to the cirrus-sac, i.e., preporal, in C. gillesi vs. posterior, i.e., only postporal in C. janikae), size of the eggs (diameter of the external layer of the embryophore of C. gillesi 30-33 μm vs. 44-46 μm in C. janikae), and the number of uterine lateral diverticula (16-21 on 1 side in C. gillesi vs. only 8-12 in C. janikae

  10. Refuge-seeking impairments mirror metabolic recovery following fisheries-related stressors in the Spanish flag snapper (Lutjanus carponotatus) on the Great Barrier Reef.

    PubMed

    Cooke, Steven J; Messmer, Vanessa; Tobin, Andrew J; Pratchett, Morgan S; Clark, Timothy D

    2014-01-01

    Fisheries and marine park management strategies for large predatory reef fish can mean that a large proportion of captured fish are released. Despite being released, these fish may experience high mortality while they traverse the water column to locate suitable refuge to avoid predators, all the while recovering from the stress of capture. The predatory reef fish Spanish flag snapper (Lutjanus carponotatus) is frequently released because of a minimum-size or bag limit or by fishers targeting more desirable species. Using L. carponotatus as a model, we tested whether simulated fishing stress (exercise and air exposure) resulted in impairments in reflexes (e.g., response to stimuli) and the ability to identify and use refuge in a laboratory arena and whether any impairments were associated with blood physiology or metabolic recovery. Control fish were consistently responsive to reflex tests and rapidly located and entered refugia in the arena within seconds. Conversely, treatment fish (exhausted and air exposed) were unresponsive to stimuli, took longer to search for refugia, and were more apprehensive to enter the refuge once it was located. Consequently, treatment fish took more than 70 times longer than control fish to enter the coral refuge (26.12 vs. 0.36 min, respectively). The finding that fish exposed to stress were hesitant to use refugia suggests that there was likely cognitive, visual, and/or physiological impairment. Blood lactate, glucose, and hematocrit measures were perturbed at 15 and 30 min after the stressor, relative to controls. However, measurements of oxygen consumption rate revealed that about 50% of metabolic recovery occurred within 30 min after the stressor, coinciding with apparent cognitive/visual/physiological recovery. Recovering the treatment fish in aerated, flow-through chambers for 30 min before introduction to the behavioral arena restored reflexes, and "recovered" fish behaved more similarly to controls. Therefore, we suggest that

  11. Growth, Age Validation, Mortality, and other Population Characteristics of the Red Emperor Snapper, Lutjanus sebae (Cuvier, 1828), off the Kimberley Coast of North-Western Australia

    NASA Astrophysics Data System (ADS)

    Newman, S. J.; Dunk, I. J.

    2002-07-01

    Red emperor, Lutjanus sebae, were examined from commercial catches in the Northern Demersal Scalefish Fishery (NDSF) of north-western Australia from 1997 to 1999. Specimens ranged from 183 to 728 mm fork length (FL); males had a mean FL of 509 mm, and were significantly larger than females that had a mean FL of 451 mm. Ages were estimated from thin sections of sagittal otoliths. Marginal increment analysis of sagittal otoliths showed a single annual minimum during September and October and indicated that one annulus is formed each year. Male L. sebae (n=977; 211-728 mm FL) ranged from age 2 to 30 years and females (n=1384; 183-584 mm FL) ranged from age 1 to 34 years. Sagittal otolith weight and height were significantly correlated with age for each sex. There was significant differential growth between sexes. The relationship of observed fork length at age was described by the von Bertalanffy growth equation for males, Lt=627·8 {1-exp [-0·151 (t+0·595)]} and females, Lt=482·6 {1-exp [-0·271 (t-0·065)]}. The slow growth, long life span and large size and age at maturity of L. sebae indicate that this species has a low production potential and hence spatial area closures are vulnerable to over-exploitation. The instantaneous rate of natural mortality (M) ranged from 0·104 to 0·122. The optimum rate of fishing mortality was estimated to be 0·052-0·061. The instantaneous rate of total mortality (Z) estimated from catch at age data for fully recruited ages, was 0·374 in 1997/98 and 0·242 in 1998/99. Hence, the NDSF population of L. sebae is exploited above optimum levels. Given their low production potential, populations of L. sebae in north-western Australia and elsewhere in the Indo-Pacific region require prudent management. Furthermore, fishery managers need to consider as part of any harvest strategy for these fish to preserve significant levels of the spawning stock.

  12. Brain and sense organ anatomy and histology of two species of phyletically basal non-Antarctic thornfishes of the Antarctic suborder Notothenioidei (Perciformes: Bovichtidae).

    PubMed

    Eastman, Joseph T; Lannoo, Michael J

    2007-06-01

    The predominantly non-Antarctic family Bovichtidae is phyletically basal within the perciform suborder Notothenioidei, the dominant component of the Antarctic fish fauna. In this article we focus on the South Atlantic bovichtids Bovichtus diacanthus, the klipfish from tide pools at Tristan da Cunha, and Cottoperca gobio, the frogmouth from the Patagonian shelf and Falkland Islands. We document the anatomy and histology of the brains, olfactory apparatus, retina, and cephalic lateral line system. We also use the microvascular casting agent Microfil to examine ocular vascular structures. We provide detailed drawings of the brains and cranial nerves of both species. Typical of perciforms, the brains of both species have a well-developed tectum and telencephalon and robust thalamic nuclei. The telencephalon of C. gobio is prominently lobed, with the dorsomedial nucleus more conspicuous than in any other notothenioid. The corpus cerebelli is relatively small and upright and, unlike other notothenioids, has prominent transverse sulci on the dorsal and caudal surfaces. Areas for lateral line mechanoreception (eminentia granularis and crista cerebellaris) are also conspicuous but olfactory, gustatory, and somatosensory areas are less prominent. The anterior lateral line nerve complex is larger than the posterior lateral line nerve in B. diacanthus, and in their cephalic lateral line systems both species possess branched membranous tubules (which do not contain neuromasts) with small pores. These are especially complex in B. diacanthus where they become increasingly branched and more highly pored in progressively larger specimens. Superficial neuromasts are sparse. Both species have duplex (cone and rod) retinae that are 1.25-fold thicker and have nearly 5-fold more photoreceptors and than those of most Antarctic notothenioids. Convergence ratios are also high for bovichtids. Bovichtus diacanthus has a yellow intraocular filter in the dorsal aspect of the cornea. Both

  13. Myxobolus lepomis n. sp. (Cnidaria: Myxobolidae), a gill myxozoan infecting Lepomis marginatus Holbrook and Lepomis miniatus Jordan (Perciformes: Centrarchidae), in the Big Thicket National Preserve, Texas, USA.

    PubMed

    Rosser, Thomas G; Baumgartner, Wes A; Barger, Michael A; Griffin, Matt J

    2017-05-01

    A parasitological survey of freshwater fishes in the Big Thicket National Preserve in southeast Texas revealed myxozoan infections in two species of sunfish, Lepomis marginatus Holbrook and Lepomis miniatus Jordan (Perciformes: Centrarchidae). Pseudocysts were elongate-oval, 988 × 485 µm (ex L. marginatus) and 800 × 606 µm (ex L. miniatus) and demonstrated a predilection to the edge of the primary gill lamellae. Myxospores consistent with the genus Myxobolus were oblong, 16.8-21.3 (19.0 ± 0.9) µm long, 7.0-8.8 (7.9 ± 0.5) µm wide and 5.3-6.1 (5.8 ± 0.3) µm thick (ex L. marginatus) and 17.2-20.3 (18.8 ± 0.7) µm long, 7.5-9.9 (8.7 ± 0.6) µm wide, and 6.8-7.2 (7.0 ± 0.2) µm thick (ex L. miniatus); with 2 pyriform polar capsules 8.3-9.8 (9.0 ± 0.5) µm long, 2.2-2.7 (2.5 ± 0.2) µm wide (ex L. marginatus) and 9.2-10.5 (10.0 ± 0.4) µm long, 2.2-3.0 (2.8 ± 0.2) µm wide (ex L. miniatus). Statistically, the measurements of spore body width, polar capsule length, and polar capsule width were significantly different between myxospores from L. marginatus and L. miniatus. However, intraspecific genetic variability between isolates at the 18S rRNA gene was negligible, with < 0.8% variability across > 2,000 bp of sequence. The isolates shared no significant sequence similarity with any myxozoan deposited in the GenBank nucleotide database. Phylogenetic analysis inferred from the 18S rRNA gene from both L. marginatus and L. miniatus placed the isolates within a clade of myxozoan parasites of perciform fishes. Based on shared tissue and host family tropism, overlapping morphological characters and high degrees of sequence conservation at the 18S rRNA gene, we propose these isolates as morphologically distinct, genetically conspecific representatives of M. lepomis n. sp. from the gills of L. marginatus and L. miniatus in the Big Thicket National Preserve in Texas, USA.

  14. Redescription and new host record of Capsala laevis (Monogenoidea: Capsalidae: Capsalinae) from gill of roundscale spearfish, Tetrapturus georgii (Perciformes: Istiophoridae) in the northwestern Atlantic Ocean.

    PubMed

    Barse, Ann M; Bullard, Stephen A

    2012-08-01

    Specimens of a capsalid collected from the gill arches of 2 roundscale spearfish, Tetrapturus georgii Lowe, 1840, (Perciformes: Istiophoridae), captured in the northwestern Atlantic Ocean were identified as Capsala laevis (Verrill, 1875) Johnston, 1929 by having the combination of papillae on the ventral surface of haptor, dorsomarginal body sclerites in a single column extending the entire body length, haptoral accessory sclerites, conical papillae distributing over the ventral body surface, and an anterior attachment organ with a fimbriated posterior margin. The new specimens plus the holotype were used to conduct a taxonomic redescription of C. laevis using light and scanning electron microscopy. We documented that the holotype (USNPC No. 7179) and the new specimens of C. laevis from roundscale spearfish each had papillae on the ventral surface of the anterior attachment organs and sensory papillae on the dorsal body surface. Although data are insufficient at this time to justify proposal of a new species, the new specimens differed from the holotype and published accounts of C. laevis by having a sinistral dorsomarginal patch comprising 27-35 sclerites whereas the holotype has a dorsomarginal patch comprising 60 sclerites. Capsala laevis morphologically most closely resembles Capsala ovalis (Goto, 1894) Price, 1938 , but can be most easily differentiated from it by having dorsomarginal body sclerites. This represents the first record of any parasite from the recently taxonomically resurrected roundscale spearfish, long considered by some as a junior subjective synonym of white marlin, Tetrapturus albidus Poey, 1860 and, concomitantly, a new host record for Capsalidae Baird, 1853. An updated list of host records for C. laevis is provided. A perusal of that literature reveals that the identity of the type host for C. laevis is indeterminate beyond Istiophoridae species and that subsequent reports of the type host as ' T. albidus ' are presumptuous (originally

  15. Complete mitochondrial genome sequence of Tridentiger bifasciatus and Tridentiger barbatus (Perciformes, Gobiidae): a mitogenomic perspective on the phylogenetic relationships of Gobiidae.

    PubMed

    Jin, Xiaoxiao; Wang, Rixin; Wei, Tao; Tang, Da; Xu, Tianjun

    2015-01-01

    The fishes of suborder Gobioidei is the largest group of those in present living Perciformes, which contains about 2,200 species belonging to 270 genera of 9 families in the world. The monophyly and phylogenetic relationships of gobies have been controversial and disputable for a long time. In the present study, the complete mitochondrial genome of the shimofuri goby Tridentiger bifasciatus (T. bifasciatus) and shokihaze goby Tridentiger barbatus (T. barbatus) were firstly determined. The two mitochondrial genomes were both consisted of 2 ribosomal RNA (rRNA) genes, 13 protein-coding genes, 22 transfer RNA (tRNA) genes, and one major control region (CR). They shared similar features with those of other gobies in terms of gene arrangement, base composition, and tRNA structures. The CR was absence of typical conserved blocks (CSB-E, and CSB-F) respectively for the T. bifasciatus and T. barbatus. Phylogenomic analyses, which based on 12 concatenated protein-coding genes and complete mitochondrial genome sequences, revealed that there were two groups within the Gobiidae. A large group consisted of the Amblyopinae, Gobionellinae, Oxudercinae and Sicydiinae, and Amblyopinae was nested in Oxudercinae and they were both paraphyletic to Sicydiinae. The other group was the Gobiinae. As a whole, our phylogenetic data was different from the traditionally classification of Gobiidae, but supported the new phylogenetic taxonomy view of Thacker (Copeia 2009:93-104, 2009).

  16. Localization and possible function of nrF-AGP, an alpha-1-acid glycoprotein-like protein in viviparous fish Neoditrema ransonnetii (Perciformes, Embiotocidae).

    PubMed

    Nakamura, Osamu; Watabe, Yuki; Matsumoto, Naoko; Takasugi, Osamu; Watanabe, Ayako; Tsutsui, Shigeyuki

    2014-12-01

    The nrF-AGP, a 51-kDa acidic glycoprotein found in surfperch (Neoditrema ransonnetii; Perciformes, Embiotocidae), is a member of the lipocalin superfamily. nrF-AGP is the major component in ovarian cavity fluid (OCF), but not in plasma of pregnant females, which suggests its potential relevance in pregnancy. However, its production in the liver, irrespective of reproductive cycle and sex, indicates that the protein also has physiological functions other than its contribution to reproduction. In the present study, Western blot analysis indicated that this protein is widely distributed in the cutaneous and intestinal mucosa, bile, and abdominal adipose tissue of fish, as well as plasma and OCF. Immunohistochemical staining of nrF-AGP was observed in hepatocytes, adipocytes, pancreatic cells, epidermal cells, and epithelial cells of ovigerous lamellae. Transcripts were detected in adipose tissue as well as hepatocytes by reverse transcription PCR analysis. This broad distribution of nrF-AGP suggests that this protein participates in various biological processes through its ability to bind to hydrophobes. After administration of biotinylated F-AGP into the ovarian cavity, the protein was detected in the cytoplasm of the intestinal epithelial cells of the fetus within 4 h. This suggests that nrF-AGP in the ovarian cavity acts as a transporter delivering maternal resources to the fetus.

  17. New and previously described dactylogyrid species (Monogenoidea: Polyonchoinea) and a gastrocotylinean pre-adult (Heteronchoinea) from pomacentrid and caesionid (Perciformes) fishes from Lizard Island, Great Barrier Reef, Australia.

    PubMed

    Mendoza-Franco, Edgar F; Binning, Sandra A; Roche, Dominique G

    2017-09-26

    During a parasitological survey of perciform fishes from Lizard Island, Great Barrier Reef, Australia, we found the following gill monogenoidean species (Platyhelminthes): Euryhaliotrema lizardi n. sp. on Caesio caerulaurea Lacepède, 1801 (Caesionidae) (type host) and Haliotrema weberii n. sp. on Chromis weberi Fowler and Bean, 1928 (Pomacentridae) (type host), Chromis amboinensis (Bleeker, 1871), Chromis atripectoralis Welander and Schultz, 1951 and Caesio teres Seale, 1906. Euryhaliotrema lizardi n. sp. is characterized by having anchors with an elongated straight shaft and point as well as a vaginal canal with two loops before connecting to the seminal receptacle. Ha. weberii n. sp. is distinguished from other congeners by possessing a tubular male copulatory organ (MCO), partially straight. Two previously described dactylogyrids were also found: Haliotrematoides caesionis (Yamaguti, 1953) Kritsky, Yang and Sun 2009) on Caesio cuning (Bloch, 1791) and Ca. teres (new host record) and Haliotrematoides patellacirrus (Bychowsky and Nagibina, 1971) Kritsky, Yang and Sun 2009, on Ca. cuning (new host record). Finally, we provide the first report of a gastrocotylinean pre-adult on Ca. teres. We provide descriptions and illustrations of the new species and the gastrocotylinean pre-adult and include supplemental observations of Ht. caesionis and Ht. patellacirrus. The present findings expand the known spectrum of host species of Euryhaliotrema, Haliotrema and Haliotrematoides to include new caesionid and pomacentrid fishes.

  18. Chronic kisspeptin administration stimulated gonadal development in pre-pubertal male yellowtail kingfish (Seriola lalandi; Perciformes) during the breeding and non-breeding season.

    PubMed

    Nocillado, Josephine N; Zohar, Yonathan; Biran, Jakob; Levavi-Sivan, Berta; Elizur, Abigail

    2013-09-15

    The kisspeptin system is now accepted as a key regulator of vertebrate reproductive function, particularly the onset of puberty. In teleosts, the stimulatory effect of exogenous kisspeptins has been demonstrated mainly at the hypothalamic and pituitary levels of the reproductive axis, with very limited information pertaining to gonadal response. We determined the effect of chronic peripheral administration of the conserved kisspeptin decapeptides (YNLNSFGLRY or Kiss1-10; and FNFNPFGLRF or Kiss2-10) on gonadal development of pre-pubertal yellowtail kingfish (Seriola lalandi), a Perciform teleost, during the breeding and non-breeding season. We utilized slow-release implants to chronically deliver the synthesized peptides, which were based on the yellowtail kingfish kiss1 and kiss2 cDNA sequences that we isolated. The expression level of kiss2r and gnrh1 in the brain or hypothalamus did not vary between treated and control groups. Pituitary expression of fshβ and lhβ was upregulated only with Kiss1-10 treatment regardless of the season. Based on histological evidence, gonadal development was stimulated in male fish with either Kiss1-10 or Kiss2-10, with Kiss2-10 being more effective during the non-breeding period. Overall, our results suggest that kisspeptins modulate the early gonadal development of male yellowtail kingfish, however that may vary with the breeding season.

  19. Redescription of Henneguya chaudhuryi (Bajpai & Haldar, 1982) (Myxosporea: Myxobolidae), infecting the gills of the freshwater fish Channa punctata (Bloch) (Perciformes: Channidae) in India.

    PubMed

    Chaudhary, Anshu; Molnár, Kálmán; Gupta, Abhishek; Cech, Gábor; Singh, Hridaya S; Székely, Csaba

    2017-03-01

    During a survey of myxosporean parasites of freshwater fishes in Meerut, Uttar Pradesh (UP), India, spores of Henneguya chaudhuryi (Bajpai & Haldar, 1982) were found in the gill lamellae of the spotted snakehead fish Channa punctata (Bloch) (Perciformes: Channidae). This species was described lacking several characteristics in the original description, which makes challenging the accurate diagnosis. Here, we supplemented its description based on morphological, histological and molecular data. Plasmodia of H. chaudhuryi are oval, measuring 60-100 × 40-68 µm, located intralamellarly. Mature spores are elongate, measuring 10.5-13.2 × 3.6-4.2 µm, with two slightly unequal polar capsules with 6-7 filamental turns and two straight, equal caudal appendages, 10-17 µm long. Scanning electron microscopy revealed a flat surface. The 18S rDNA sequence for H. chaudhuryi did not show a close relationship with those of any other Henneguya spp., represented in the GenBank.

  20. A new species of Heterosentis Van Cleave, 1931 (Acanthocephala: Arhythmacanthidae) parasitic in Pseudopercis numida Miranda Ribeiro, 1903 (Perciformes: Pinguipedidae) from southeastern Brazilian coastal zone.

    PubMed

    Vieira, Fabiiano M; Felizardo, Nilza N; Luque, José L

    2009-06-01

    Heterosentis brasiliensis n. sp. (Acanthocephala, Arhythmacanthidae), parasitic in namorado sandperch Pseudopercis numida Miranda-Ribeiro, 1903 (Perciformes, Pinguipedidae) from the littoral of Cabo Frio, State of Rio de Janeiro, Brazil, is described and illustrated herein. Heterosentis brasiliensis n. sp. differs from all congeneric species by having 10 longitudinal rows of hooks in the proboscis, 6 or 7 hooks in each row, 3 or 4 small basal hooks in each row, and spines in the anterior ventral surface of the body. The similar species, Heterosentis heteracanthus (Linstow, 1896) and Heterosentis caballeroi Gupta & Fatma, 1983, also have 10 longitudinal rows of hooks, but H. heteracanthus differs from the new species by possessing trunk spines in the ventral and dorsal body surface. Heterosentis caballeroi differs from H. brasiliensis by the presence of 1 apical and 1 subapical hook in each longitudinal row; the largest apical, subapical, and basal hooks; lemnisci that are smaller than the proboscis receptacles; and a pre-equatorial male reproductive system. This is the first record of a Heterosentis species in a pinguipedid fish and from Brazilian coastal zone.

  1. Caligus lini n.sp., a new caligid (Copepoda, Siphonostomatoida) parasitic on the brilliant pomfret Eumegistus illustris Jordan & Jordan (Perciformes, Bramidae) of Taiwan.

    PubMed

    Ho, Ju-Shey; Cheng, Yu-Rong

    2016-02-10

    Caligus lini n.sp., a new species of caligid copepod parasitic on the gill rakers and caudal fin of the brilliant pomfret, Eumegistus illustris Jordan & Jordan (Perciformes, Bramidae), is described from fish caught off Tai-Dong, Taiwan. The new species differs from its congeners by its possession of the following, unique combination of features: (1) middle two elements at the tip of leg 1 simple (without accessory process), (2) innermost element shortest and smallest of the four terminal elements on leg 1, (3) leg 4 exopod with an armature formula of I,III, and (4) complex leg 5 represented by 2 seta-bearing processes, a simple anterior process tipped with 1 plumose seta and quadripartite posterior process carrying 3 setae (1 simple and 2 plumose). The new species differs from its closest congener, Caligus tylosuri (Rangnekar, 1956), in the structure of the middle two elements (simple without carrying accessory process) at the tip of leg 1 and a quadripartite (instead of tripartite) posterior process on leg 5.

  2. Identification of Two Subgroups of Type I IFNs in Perciforme Fish Large Yellow Croaker Larimichthys crocea Provides Novel Insights into Function and Regulation of Fish Type I IFNs

    PubMed Central

    Ding, Yang; Ao, Jingqun; Huang, Xiaohong; Chen, Xinhua

    2016-01-01

    Like mammals, fish possess an interferon regulatory factor (IRF) 3/IRF7-dependent type I IFN responses, but the exact mechanism by which IRF3/IRF7 regulate the type I IFNs remains largely unknown. In this study, we identified two type I IFNs in the Perciforme fish large yellow croaker Larimichthys crocea, one of which belongs to the fish IFNd subgroup and the other is assigned to a novel subgroup of group I IFNs in fish, tentatively termed IFNh. The two IFN genes are constitutively expressed in all examined tissues, but with varied expression levels. Both IFN genes can be rapidly induced in head kidney and spleen tissues by polyinosinic–polycytidylic acid. The recombinant IFNh was shown to be more potent to trigger a rapid induction of the antiviral genes MxA and protein kinase R than the IFNd, suggesting that they may play distinct roles in regulating early antiviral immunity. Strikingly, IFNd, but not IFNh, could induce the gene expression of itself and IFNh through a positive feedback loop mediated by the IFNd-dependent activation of IRF3 and IRF7. Furthermore, our data demonstrate that the induction of IFNd can be enhanced by the dimeric formation of IRF3 and IRF7, while the IFNh expression mainly involves IRF3. Taken together, our findings demonstrate that the IFN responses are diverse in fish and are likely to be regulated by distinct mechanisms. PMID:27656183

  3. Philometra johnii sp. nov. (Nematoda, Philometridae), a new gonad-infecting philometrid from the sin croaker Johnius dussumieri (Cuvier) (Perciformes, Sciaenidae) from marine waters of Iraq.

    PubMed

    Moravec, František; Ali, Atheer Hussain

    2013-09-01

    A new nematode species, Philometra johnii sp. nov. (Philometridae), is described from male and female specimens found in the ovary of the sin croaker, Johnius dussumieri (Cuvier) (Perciformes, Sciaenidae), in the Arabian Gulf, off the southern coast of Iraq. Based on light and scanning electron microscopical examination, the new species differs from all other gonad-infecting Philometra spp. with dorsal lamella-like structures on the distal tip of the gubernaculum in having a dorsal bipartite protuberance formed by two subdorsal lamellated parts separated from each other by a smooth longitudinal field. This gubernaculum structure is unique among all philometrids. Other distinguishing features include the structure of the male tail, number (6 pairs) and arrangement of genital papillae and the length of spicules (102-153 μm). From eight congeneric, gonad-infecting species with unknown males, the new species can be distinguished by some morphological and biometrical features found in gravid females (absence of caudal projections, length of oesophagus, moderately developed anterior oesophageal inflation, length of first-stage larvae) by the host type (fish family) and geographical distribution. Philometra johnii sp. nov. is the fourth known philometrid species recorded from marine waters of Iraq.

  4. Pseudobacciger cheneyae n. sp. (Digenea: Gymnophalloidea) from Weber's chromis (Chromis weberi Fowler & Bean) (Perciformes: Pomacentridae) at Lizard Island, Great Barrier Reef, Australia.

    PubMed

    Sun, Derek; Bray, Rodney A; Yong, Russell Q-Y; Cutmore, Scott C; Cribb, Thomas H

    2014-06-01

    A new species of digenean, Pseudobacciger cheneyae n. sp., is described from the intestines of Weber's chromis (Chromis weberi Fowler & Bean) from off Lizard Island, Great Barrier Reef, Australia. This species differs from the three described species of Pseudobacciger Nahhas & Cable, 1964 [P. cablei Madhavi, 1975, P. harengulae Yamaguti, 1938 and P. manteri Nahhas & Cable, 1964] in combinations of the size of the suckers and the length of the caeca. The host of the present species is a perciform (Family Pomacentridae) which contrasts with previous records of the genus which are almost exclusively from clupeiform fishes. The genus Pseudobacciger is presently recognised within the family Faustulidae (Poche, 1926) but phylogenetic analyses of 28S and ITS2 rDNA show that the new species bears no relationship to species of four other faustulid genera (Antorchis Linton, 1911, Bacciger Nicoll, 1924, Paradiscogaster Yamaguti, 1934 and Trigonocryptus Martin, 1958) but that instead it is nested within the Gymnophalloidea (Odhner, 1905) as sister to the Tandanicolidae (Johnston, 1927). This result suggests that the Faustulidae is polyphyletic.

  5. The barred grunt Conodon nobilis (Perciformes: Haemulidae) in shallow areas of a tropical bight: spatial and temporal distribution, body growth and diet

    NASA Astrophysics Data System (ADS)

    Pombo, Maíra; Denadai, Márcia Regina; Bessa, Eduardo; Santos, Flávia Borges; de Faria, Vanessa Hermann; Turra, Alexander

    2014-06-01

    This study aimed to comprehensively investigate the population biology of Conodon nobilis (Perciformes, Haemulidae) in Caraguatatuba Bight, southeastern Brazil. Monthly trawls were performed from October 2003 through October 2004 in two areas of the bight that are similar to but distant from each other, South and North. For all specimens, the size was measured and the sex and reproductive stage identified. Abundance and size were compared over areas and months. Body growth parameters were parameterized according to the Von Bertalanffy growth function. The stomach contents were identified and quantified. C. nobilis occurred mainly in the North area and showed an erratic pattern of abundance over time. Several cohorts entered in different periods, but very few large and mature individuals were observed. The results indicate a preference for shallow, ocean-influenced habitats and some degree of segregation between young and older individuals. The species showed a distribution consistent with an r-strategist species, with high abundance and a high growth constant ( K = 0.68 year-1 and L max = 34.2 cm). Both the relative length of the digestive tube and the prey items indicated a carnivorous feeding habit; mysids were the main item of the diet throughout the study period, indicating that this grunt is a specialist feeder. Other frequently observed items were amphipods and fish fragments. Ingestion of scales is possibly intentional.

  6. Morphological and molecular evidence for a new species of the genus Dichelyne Jägerskiöld, 1902 (Ascaridida: Cucullanidae) from marine perciform fishes in the South China Sea.

    PubMed

    Li, Liang; Xu, Zhen; Zhang, Lu-Ping

    2014-10-01

    A new cucullanid nematode, Dichelyne (Dichelyne) breviculus n. sp., collected from the intestine of the goatee croaker Dendrophysa russelii (Cuvier) (Perciformes: Sciaenidae), the burrowing goby Trypauchen vagina (Bloch & Schneider) and the tropical sand goby Acentrogobius caninus (Valenciennes) (Perciformes: Gobiidae) in the South China Sea, is described using both light and scanning electron microscopy. The new species differs from its congeners in the size of body (2.16-2.96 mm in male), the position of the excretory pore and deirids, the length of the spicules (0.90-1.32 mm, representing 32.4-51.9% of body length), the arrangement of the caudal papillae and the morphology of the tail. In addition, in order to primarily assess the validity of the new species genetically, the specimens of D. breviculus n. sp. collected from the three different hosts were also characterised using molecular methods by sequencing and analysing ribosomal [small ribosomal subunit (18S rDNA) and internal transcribed spacer (ITS)] and mitochondrial [cytochrome c oxidase subunit 1 (cox1)] target regions. The molecular analyses support the validity of the new species based on the morphological observations.

  7. Genomic organization and functional diversification of two warm-temperature-acclimation-associated 65-kDa protein genes in rockbream (Oplegnathus fasciatus; Perciformes).

    PubMed

    Lee, Sang Yoon; Kim, Byoung Soo; Noh, Choong Hwan; Nam, Yoon Kwon

    2014-03-01

    Two paralogue genes of warm-temperature-acclimation-associated 65-kDa protein were characterized and their mRNA expression patterns during various experimental stimulations were examined in the rockbream (Oplegnathus fasciatus; Perciformes). Rockbream Wap65 isoforms (rbWap65-1 and rbWap65-2) share basically common structural features with other teleostean orthologues and human hemopexin (HPX) at both amino acid (conserved cysteine and histidine residues) and genomic levels (ten-exon structure), although the rbWap65-2 reveals more homologous characteristics to human HPX than does rbWap65-1 isoform. Southern blot analysis indicates that each rbWap65 isoform exists as a single copy gene in the rockbream genome. Both rbWap65 genes were predicted to possess various transcription factor (TF) binding motifs related with stress and innate immunity in their 5ʹ-upstream regions, in which inflammation-related motifs were more highlighted in the rbWap65-2 than in rbWap65-1. Based on the RT-PCR assay, the liver-predominant expression pattern was more apparent in rbWap65-1 than rbWap65-2 isoform. During thermal elevation, clear upregulation was found only for the rbWap65-1. In contrast, immune stimulations (bacterial challenges, viral infection and iron overload) activated more preferentially the rbWap65-2 isoform in overall, although the inducibility was affected by the kinds of stimulators and tissue types. Taken together, our data suggest that the two paralogue rbWap65 isoforms have experienced subfunctionalization and/or neofunctionalization during their evolutionary history, in which the rbWap65-2 has retained closer, functional orthology to the human HPX while the rbWap65-1 have been diversified to be more related with thermal acclimation physiology.

  8. A new aporocotylid (Digenea) species from blood vascular system of gag grouper, Mycteroperca microlepis (Perciformes: Serranidae), off Alabama, with an emendation of Pearsonellum Overstreet & Køie, 1989.

    PubMed

    Bullard, Stephen A

    2012-04-01

    Pearsonellum lemusi n. sp. (Digenea: Aporocotylidae) infects the blood vascular system of the gag grouper, Mycteroperca microlepis (Perciformes: Serranidae), in the north central Gulf of Mexico, approximately 80 km south of Dauphin Island, Alabama (29°34'09″N, 88°22'16″W). The new species can be most easily differentiated from its only congeners Pearsonellum corventum Overstreet and Køie, 1989 (type species) and Pearsonellum pygmaeus Nolan and Cribb, 2004 , both of which infect Australian serranids, by the combination of having a large adult body (3,237 × 570 µm), a cecal intersection comprising an elongated medial channel, anterior ceca >10% of total body length, ovary narrower than testis, and pre-ovarian uterus not looping between testis and ovary. The embryonated eggs of the new species infect gill epithelium, are spheroid, and measure 25-30 µm in diameter. Sympatric Gulf of Mexico serranids were negative for aporocotylid infections: coney, Cephalopholis fulva (n  =  1); Nassau grouper, Epinephelus striatus (3); red grouper, Epinephelus morio (32); yellowedge grouper, Epinephelus flavolimbatus (1); rock hind, Epinephelus adscensionis (1); red hind, Epinephelus guttatus (2); Warsaw grouper, Epinephelus nigritus (3); graysby, Cephalopholis cruentata (1); black grouper, Mycteroperca bonaci (1), and tattler, Serranus phoebe (2). The new species is the first aporocotylid described from a serranid outside of the southwestern Pacific Ocean. The diagnosis of Pearsonellum Overstreet and Køie, 1989 is herein emended to include anterior sucker having concentric rows of spines anterior to mouth, pharynx absent, esophagus length <1/2 total body length, vas deferens connecting with cirrus sac anteromedially, ovary occupying posterior 1/4-1/3 of body, primary vitelline duct dextral, and oviducal seminal receptacle extending posteriad in parallel with lateral body margin, not transverse nor constricted anteriorly or posteriorly by sharp bends or kinks.

  9. [Comparative analysis of three length based methods for estimating growth of the tilapia Oreochromis aureus (Perciformes: Cichlidae) in a tropical lake of Mexico].

    PubMed

    Arellano-Torres, Andrés; Hernández Montaño, Daniel; Meléndez Galicia, Carlos

    2013-09-01

    A comparative analysis of three length based methods for estimating growth of the tilapia Oreochromis aureus (Perciformes: Cichlidae) in a tropical lake of Mexico. Several methods are now available to estimate fish individual growth based upon the distribution of body lengths in a population. Comparative analyses of length-based methods have been undertaken mainly for marine species; nevertheless, limited information is available for inland species. Tilapia is one of the most important freshwater fisheries and its growth parameters have been estimated by several authors, usually using one length-based method. Thus, the main objectives of this study were: a) to estimate growth parameters of O. aureus from Chapala lake, Mexico, using three length-based methods ELEFAN, PROJMAT and SLCA; b) to quantify the effect of input data variations in growth parameters estimates by the jackknife technique; and c) to compare the new estimates with those previously reported, through the standard growth index phi. We collected and analyzed a total of 1,973 specimens from commercial landings from January to December 2010. The three length-base methods used in the present study resulted in parameter estimates within the range of those reported in other studies. Results derived from jackknife analysis revealed lowest values in the error percentage and coefficient of variation for L infinity when applying ELEFAN, while PROJMAT showed lowest values in the precision estimators for K, which was very similar to ELEFAN. Estimates of the comparative growth index phi were also very similar to those reported for the same species when studied in different reservoirs. Considering our results, we suggest the use of ELEFAN rather than SLCA due to its accuracy to estimate growth parameters for O. aureus.

  10. Differential Modulation of IgT and IgM upon Parasitic, Bacterial, Viral, and Dietary Challenges in a Perciform Fish.

    PubMed

    Piazzon, Maria C; Galindo-Villegas, Jorge; Pereiro, Patricia; Estensoro, Itziar; Calduch-Giner, Josep A; Gómez-Casado, Eduardo; Novoa, Beatriz; Mulero, Victoriano; Sitjà-Bobadilla, Ariadna; Pérez-Sánchez, Jaume

    2016-01-01

    Three different immunoglobulin (Ig) isotypes can be found in teleost fish, IgM, IgD, and the teleost-specific IgT. IgM is considered to have a systemic activity, and IgT is attributed a mucosal role, similar to mammalian IgA. In this study, the complete sequence of gilthead sea bream IgM and IgT in their membrane (m) and soluble (s) forms are described for the first time in a perciform fish. Their constitutive gene expression is analyzed in different tissues, and their regulation upon viral, bacterial, parasitic, mucosal vaccination and dietary challenges are studied. GCB IgM and IgT have the prototypical structure when compared to other fish Igs. The constitutive expression of sIgM was the highest overall in all tissues, whereas mIgT expression was highest in mucosal tissues, such as gills and intestine. IgM and IgT were differentially regulated upon infection. IgT was highly upregulated locally upon infection with the intestinal parasite Enteromyxum leei or systemically after Nodavirus infection. Long-term intestinal parasitic infections increased the serum titer of both isotypes. Mucosal vaccination against Photobacterium damselae subsp. piscicida finely regulated the Ig response inducing a systemic increase of IgM titers in serum and a local IgT response in skin mucus when animals were exposed to the pathogen by bath challenge. Interestingly, plant-based diets inhibit IgT upregulation upon intestinal parasitic challenge, which was related to a worse disease outcome. All these results corroborate the mucosal role of IgT and emphasize the importance of a finely tuned regulation of Ig isotypes upon infection, which could be of special interest in vaccination studies.

  11. Six new and one previously described species of Pseudorhabdosynochus (Monogenoidea, Diplectanidae) infecting the gills of groupers (Perciformes, Serranidae) from the Pacific coasts of Mexico and Panama.

    PubMed

    Mendoza-Franco, Edgar F; Violante-González, Juan; Rojas Herrera, Agustín A

    2011-02-01

    Six new and 1 previously described species of Pseudorhabdosynochus (Diplectanidae) are described and/or reported from the gill lamellae of 5 serranid (Perciformes) fish species from the Pacific waters in Guerrero State of Mexico and Panama City, Panama. These species are Pseudorhabdosynochus guerreroensis n. sp. from the Pacific mutton hamlet Alphestes inmaculatus Breder (type host), rivulated mutton hamlet Alphestes multiguttatus (Günther), and spotted grouper Epinephelus analogus Gill from Mexico; Pseudorhabdosynochus urceolus n. sp. from the Pacific graysby Cephalopholis panamensis (Steindachner) from Taboga Island in Panama; Pseudorhabdosynochus spirani n. sp. from the starry grouper Epinephelus labriformis (Jenyns) from Mexico and the Perlas Archipelago and Taboga Island in Panama; Pseudorhabdosynochus fulgidus n. sp. from E. labriformis from Mexico and the Perlas Archipelago and Taboga Island (type locality) in Panama; Pseudorhabdosynochus tabogaensis n. sp. from E. labriformis from Mexico and the Perlas Archipelago and Taboga Island (type locality) in Panama; Pseudorhabdosynochus anulus n. sp. from E. labriformis from Mexico and Taboga Island (type locality) in Panama; and Pseudorhabdosynochus amplidiscatum (Bravo-Hollis, 1954) Kritsky and Beverley-Burton, 1986 from E. analogus and E. labriformis from Mexico and the Perlas Archipelago and Taboga Island in Panama. All new species are mainly distinguished from other species of the genus by the shape and size of the sclerotized vagina and haptoral structures. The present specimens of Alphestes, Cephalopholis, and Epinephelus spp. represent new host records and Panama represents a new geographic record for species of Pseudorhabdosynochus. The apparent common feature supporting a close similarity of these diplectanids is a single, secondary ejaculatory bulb with thickened wall.

  12. Differential Modulation of IgT and IgM upon Parasitic, Bacterial, Viral, and Dietary Challenges in a Perciform Fish

    PubMed Central

    Piazzon, Maria C.; Galindo-Villegas, Jorge; Pereiro, Patricia; Estensoro, Itziar; Calduch-Giner, Josep A.; Gómez-Casado, Eduardo; Novoa, Beatriz; Mulero, Victoriano; Sitjà-Bobadilla, Ariadna; Pérez-Sánchez, Jaume

    2016-01-01

    Three different immunoglobulin (Ig) isotypes can be found in teleost fish, IgM, IgD, and the teleost-specific IgT. IgM is considered to have a systemic activity, and IgT is attributed a mucosal role, similar to mammalian IgA. In this study, the complete sequence of gilthead sea bream IgM and IgT in their membrane (m) and soluble (s) forms are described for the first time in a perciform fish. Their constitutive gene expression is analyzed in different tissues, and their regulation upon viral, bacterial, parasitic, mucosal vaccination and dietary challenges are studied. GCB IgM and IgT have the prototypical structure when compared to other fish Igs. The constitutive expression of sIgM was the highest overall in all tissues, whereas mIgT expression was highest in mucosal tissues, such as gills and intestine. IgM and IgT were differentially regulated upon infection. IgT was highly upregulated locally upon infection with the intestinal parasite Enteromyxum leei or systemically after Nodavirus infection. Long-term intestinal parasitic infections increased the serum titer of both isotypes. Mucosal vaccination against Photobacterium damselae subsp. piscicida finely regulated the Ig response inducing a systemic increase of IgM titers in serum and a local IgT response in skin mucus when animals were exposed to the pathogen by bath challenge. Interestingly, plant-based diets inhibit IgT upregulation upon intestinal parasitic challenge, which was related to a worse disease outcome. All these results corroborate the mucosal role of IgT and emphasize the importance of a finely tuned regulation of Ig isotypes upon infection, which could be of special interest in vaccination studies. PMID:28082977

  13. Molecular characterization and new geographical record of Lecithochirium priacanthi (Digenea: Hemiuridae) infecting the moontail bullseye fish Priacanthus hamrur (Perciformes: Priacanthidae) from the Red Sea, Egypt.

    PubMed

    Abdel-Ghaffar, Fathy; Abdel-Gaber, Rewaida; Bashtar, Abdel-Rahman; Morsy, Kareem; Al Quraishy, Saleh; Saleh, Rehab; Mehlhorn, Heinz

    2015-12-01

    Sixty specimens of the moontail bullseye Priacanthus hamrur were collected at Coasts of Suez Gulf, Red Sea (Egypt) during the four different seasons of the whole year 2014 and necropsied to study the infection with metazoan parasites. Twenty-one out of 60 examined fish specimens (infection rate of 33.33 %) were found to be naturally infected by the hemiurid digenean parasite Lecithochirium priacanthi. The large-sized fish reaching 15-30 (23.5 ± 4.8) cm were more intensively infected than the smaller ones. A definite seasonal effect was observed as winter was found to be the season of severe parasitic infections, while midsummer was the lowest one. The morphological and morphometric characterization of this parasite were examined by light and scanning electron microscopy. The adult worms had an elongated body measuring 1.93-2.54 (2.11 ± 0.20) mm in length and 0.61-0.72 (0.67 ± 0.02) mm in width. The body was characterized by the presence of a sub-terminal oral sucker with diameters reaching 0.12-0.16 (0.14 ± 0.02) mm. The ventral sucker measured 0.32-0.45 (0.38 ± 0.02) mm in diameter. The body was supplied by a short retracted portion with a blunt end that measured 0.48-0.61 (0.56 ± 0.02) mm in length and 0.28-0.35 (0.32 ± 0.02) mm in width. Morphological results of the present parasite were compared with other related species described previously from Perciformes. Molecular characterization based on small subunit ribosomal DNA was done to confirm the obtained morphological and morphometric results. A preliminary genetic comparison between SSU rDNA of this parasite and other species of Hemiuridae places the present specimen as a putative sister taxon to Lecithochirium grandiporum and Lecithochirium caesionis. The finding of L. priacanthi in Egyptian marine water fish represents a new geographical record for this parasite.

  14. Acanthocercodes n. g. (Monogenoidea: Diplectanidae) for species parasitising threadfins (Perciformes: Polynemidae), with description of Acanthocercodes bullardi n. sp. from the Atlantic threadfin Polydactylus octonemus (Girard) and reassignment of three species of Diplectanum Monticelli, 1903 from the Indo-Pacific Ocean.

    PubMed

    Kritsky, Delane C; Diggles, Ben K

    2015-07-01

    Acanthocercodes n. g. (Diplectanidae) is proposed for Acanthocercodes bullardi n. sp. and three previously described species of Diplectanum all parasites of the gill lamellae of threadfins (Perciformes: Polynemidae). The new genus is characterised by species having peduncular spines composed of an anteriorly directed point and a flattened base from which an anterior root arises. Members of the genus lack auxiliary spinous or sucker-like structures in the haptor. Acanthocercodes bullardi n. sp. is described from the Atlantic threadfin, Polydactylus octonemus (Girard), in the Gulf of Mexico off Louisiana, USA. Diplectanum polynemus Tripathi, 1957 is redescribed and transferred to Acanthocercodes as A. polynemus (Tripathi, 1957) n. comb. based on specimens collected from the fourfinger threadfin, Eleutheronema tetradactylum (Shaw), from the mouth of the River Adelaide, Northern Territory, Australia. Diplectanum spinosum (Maillard & Vala, 1980) (=Pseudodiplectanum spinosum Maillard & Vala, 1980) and Diplectanum megacirrus (Maillard & Vala, 1980) (=Pseudodiplectanum megacirrus Maillard & Vala, 1980) from the lesser African threadfin, Galeoides decadactylus (Bloch), are transferred to Acanthocercodes as A. spinosum (Maillard & Vala, 1980) n. comb. and A. megacirrus (Maillard & Vala, 1980) n. comb., respectively.

  15. [Molecular phylogenetic study of several eelpout fishes (Perciformes, Zoarcoidei) from far eastern seas on the basis of the nucleotide sequence of the mitochondrial cytochrome oxidase 1 gene (Co-1)].

    PubMed

    Turanov, S V; kartavtseva, Iu F; Zemnukhov, V V

    2012-02-01

    A total of 95 nucleotide sequences of a Co-1 gene fragment of approximately 650 bp were analyzed for fishes of the orders Perciformes and Scorpaeniformes (outgroup). Gene trees based on four algorithms (BA, NJ, MP, and ML) were similar in topology of solved branches. An emphasis was placed on the species and generic levels, but a significant phylogenetic signal was obtained for higher taxonomic ranks as well. For instance, a monophyletic origin was confirmed for the family Zoarcidae and the subfamily Opisthocentrinae (Stichaeidae). The proportion of different nucleotides in the sequences compared (p-distances) significantly increased with increasing taxonomic rank. The p-distances were estimated for four hierarchic levels and were (1) 0.15 0.06% for the within-species hierarchic level, (2) 6.33 0.37% for the within-genus level, (3) 11.83 0.06% for the within-family level, and (4) 15.22 0.05% for the within-order level. The difference in the Co-1 gene fragments between levels (1) and (2) allows almost errorless species identification on the basis of this kind of a molecular bar code.

  16. Morphological and molecular analyses of a new species of Saccocoelioides Szidat, 1954 (Haploporidae Nicoll, 1914) in the fat sleeper Dormitator maculatus (Bloch) (Perciformes: Eleotridae) from the Gulf of Mexico.

    PubMed

    Andrade-Gómez, L; Pinacho-Pinacho, C D; Hernández-Orts, J S; Sereno-Uribe, A L; García-Varela, M

    2016-07-26

    Saccocoelioides olmecae n. sp. is described from specimens recovered from the intestine of the fat sleeper Dormitator maculatus (Bloch) (Perciformes: Eleotridae) collected in six localities along the coast of the Gulf of Mexico. The new species is mainly distinguished from the other three described species of Saccocoelioides Szidat, 1954 from North and Middle America (i.e. S. sogandaresi Lumsden, 1963, S. chauhani Lamothe-Argumedo, 1974 and S. lamothei Aguirre-Macedo & Violante-González, 2008) by having an elongated body, a sac-like caecum, a uterus that extends to the first third of body and by having vitelline follicles longitudinally elongated reaching the posterior end of the body. Sequences of the large subunit (LSU) of the ribosomal DNA, including the domain D1-D3, and the internal transcribed spacer 2 (ITS2) were used independently and concatenated to corroborate the morphological distinction among S. olmecae n. sp., S. chauhani and S. lamothei from freshwater and brackish-water fish from Middle America. The genetic divergence estimated among the three species of Saccocoelioides was very low: 1% for LSU and from 1 to 4% for ITS2. Maximum likelihood and Bayesian inference analyses for each dataset and both datasets combined revealed that S. olmecae n. sp. represents an independent clade with moderate bootstrap support and posterior probabilities. This is the third species of Saccocoelioides described in Mexico, and the 17th species from the Americas.

  17. Gene flow, population growth and a novel substitution rate estimate in a subtidal rock specialist, the black-faced blenny Tripterygion delaisi (Perciformes, Blennioidei, Tripterygiidae) from the Adriatic Sea.

    PubMed

    Koblmüller, Stephan; Steinwender, Bernd; Weiß, Sara; Sefc, Kristina M

    2015-11-01

    Population histories depend on the interplay between exogeneous and endogeneous factors. In marine species, phylogeographic and demographic patterns are often shaped by sea level fluctuations, water currents and dispersal ability. Using mitochondrial control region sequences (n = 120), we infer phylogeographic structure and historic population size changes of a common littoral fish species, the black-faced blenny Tripterygion delaisi (Perciformes, Blennioidei, Tripterygiidae) from the north-eastern Adriatic Sea. We find that Adriatic T. delaisi are differentiated from conspecific populations in the remaining Mediterranean, but display little phylogeographic structure within the Adriatic basin. The pattern is consistent with passive dispersal of planktonic larvae along cyclonic currents within the Adriatic Sea, but limited active dispersal of adults. Demographic reconstructions are consistent with recent population expansion, probably triggered by rising sea levels after the last glacial maximum (LGM). Placing the onset of population growth between the LGM and the warming of surface waters (18 000-13 000 years BP) and employing a novel expansion dating approach, we inferred a substitution rate of 2.61-3.61% per site per MY. Our study is one of only few existing investigations of the genetic structure of animals within the Adriatic basin and is the first to provide an estimate for mitochondrial control region substitution rates in blennioid fishes.

  18. A new species of Neolebouria Gibson, 1976 (Opecoelidae: Plagioporinae) from the whitecheek monocle bream, Scolopsis vosmeri (Perciformes: Nemipteridae), from the Panjim coast at Goa, with a checklist of parasites previously reported from this fish.

    PubMed

    Jaiswal, Neeshma; Upadhyay, S K; Malhotra, Anshu; Blend, Charles K; Dronen, Norman O; Malhotra, Sandeep K

    2014-05-23

    Neolebouria capoori n. sp. (Opecoelidae: Plagioporinae) is described from the whitecheek monocle bream, Scolopsis vosmeri (Bloch) (Perciformes: Nemipteridae) from the Panjim coast on the central west coast of India at Goa. The new species differs from both Neolebouria cantherhini (Li, Qiu & Zhang, 1988) as originally described from Thamnaconus modestus (Günther) (syn. Cantherines modestus Günther ) and Neolebouria confusum (Overstreet, 1969) as originally described from Ocyurus chrysurus (Bloch) by having the cirrus sac surpassing the ventral sucker posteriorly in N. cantherhini and being entirely preacetabular in N. confusum compared to terminating near the midlevel of the ventral sucker in N. capoori n. sp. The new species is most similar to N. confusum, but it further differs from this species by having the vitelline fields terminating near the level of the esophageal bifurcation compared to terminating near the level of the posterior margin of the pharynx, a larger sucker ratio (1:1.7-1:2.0 compared to 1:1.4-1:1.7), a somewhat shorter cirrus sac relative to body length (160-448, representing 9-18% of the body length compared to about 367, representing 22%), and the egg of the new species has a boss at the anopercular end that is not present in N. confusum. This study represents the first report on an opecoelid from S. vosmeri. A review of the parasites reported from S. vosmeri is included.

  19. Anatomy and histology of the brain and sense organs of the antarctic plunderfish Dolloidraco longedorsalis (Perciformes: Notothenioidei: Artedidraconidae), with comments on the brain morphology of other artedidraconids and closely related harpagiferids.

    PubMed

    Eastman, Joseph T; Lannoo, Michael J

    2003-03-01

    In the high-latitude shelf waters of Antarctica, fishes in the perciform suborder Notothenioidei dominate the fish fauna and constitute an adaptive radiation and a species flock. The 25 species of notothenioid plunderfishes, comprising four genera of the family Artedidraconidae, contribute substantially to fish species diversity on the high Antarctic shelf. A mental barbel is an autapomorphy for the family. Dolloidraco longedorsalis is the most abundant artedidraconid at depths over 400 m in these waters. In this article we present the anatomy and histology of the brain and special sense organs of Dolloidraco and compare it to the brains of other artedidraconids, closely related harpagiferids, and more generally to other notothenioids. We provide a detailed drawing of the brain and cranial nerves. The brain of Dolloidraco is simple, without external hypertrophy of sensory or motor regions, but contains several unusual features associated with the ventricular system and CSF, including well-developed circumventricular organs, subependymal expansions, and subarachnoid cisterns; and a ventricle in the corpus cerebellum. The brain of Dolloidraco also contains a lobed chief sensory nucleus of the trigeminal nerve that is correlated across species with barbel length. The eyes are large and contain a small choroid rete, a structure previously thought to be absent from members of this family. We document the histology of the duplex retina, olfactory apparatus, cutaneous taste buds, and barbel musculature and innervation. We discuss the role of pedomorphy in producing simplified brain morphologies. We consider the possibility that Dolloidraco is a somatosensory specialist-an unusual feature among vertebrates-and decide that this is unlikely. Copyright 2003 Wiley-Liss, Inc.

  20. Species profiles: Life histories and environmental requirements of coastal fishes and invertebrates (South Florida): Gray, lane, mutton, and yellowtail snappers. [Lutjanus griseus; Lutjanus synagris; Lutjanus analis; Ocyurus chrysurus

    SciTech Connect

    Bortone, S.A.; Williams, J.L.

    1986-06-01

    These four snapper species have similar morphologies as well as life history and environmental requirements. With some exceptions they are restricted to tropical and subtropical coastal areas of the western Atlantic. Adults often associate with coral reef and other hard-bottom communities. Juveniles and pre-adults usually inhabit shallow, inshore areas and are often found in seagrass beds. Reproduction usually takes place offshore in the warmer months of the year. Fecundity is positively related to female body size, but there is substantial variation both within and among the species. Juveniles obtain adult body proportions at about 50 mm SL. Approximate total maximum lengths of adults are 900 mm for gray snapper, 446 mm for lane snapper, and 750 mm for mutton and yellowtail snappers. Otoliths are the most preferred body part used to determine age. Most snappers attain sexual maturity after 3 to 5 years at a size range of 180 to 350 mm FL. Males mature at a smaller size than females. Recent commercial catches of gray and lane snappers have been increasing, whereas mutton and yellowtail snappers show no clear trends. Sport fishing probably has a substantial impact on the stocks of all four species. All four snappers feed predominantly on small benthic fishes and crustaceans, but yellowtail snapper feed on more pelagic animals. Upper water temperature tolerance limits are 27.2 to 32.5/sup 0/C and lower limits are 11 to 14/sup 0/C.

  1. Larval development of Evermannia zosterura (Perciformes: Gobiidae).

    PubMed

    González-Navarro, Enrique; Saldierna-Martínez, Ricardo Javier; Aceves-Medina, Gerardo

    2014-06-01

    Gobiidae is the most specious fish family in the world with almost 2 000 species, however only 11% of them have been described for their larval stages. The entire life cycle information is essential to understand the biology and ecology of this important fish group. Previous studies on zooplankton samples from Ensenada de La Paz, México, have shown the presence of several Gobiidae larvae and juveniles which were identified as Evermania zosterura. The main objective of this work was to describe the larval stages of this species, widely distributed in the Eastern tropical Pacific. The development of E. zosterura larvae was described based on 66 specimens. A total of 53 specimens were used to describe morphometrics and pigmentation patterns, while 13 specimens were cleared and stained, to obtain meristic characteristics. Cleared specimens had 30 to 31 total vertebrae; dorsal-fin elements: IV; 1, 13-14, anal-fin elements: 1, 13-14, and most had pterygiophore formula 4-111100. The combination of these characteristics confirmed these specimens as E. zosterura. The pigment pattern is similar throughout ontogeny. Larvae are characterized by having three to five dendritic melanophores along the post-anal ventral margin, four to nine smaller melanophores along the ventral margin between the isthmus and anus, and one on the midpoint of the dorsal margin of the tail. There is one small pigment spot on the angle of the jaw, and other on the tip of lower lip. There is an elongated internal pigment under the notochord, between the head and gas bladder. Notochord flexion starts near 3.5mm BL and ends at 4.6mm BL; transformalion to the juvenile stage is at about 13.6mm BL. Our conclusion is that the most useful characters to distinguish this species early-larval stages from those of similar species in the area, are the number of myomeres, the large melanophores (approximately uniformly in size) on the post anal ventral margin, and the elongate internal pigment under the notochord, anterior to the gas bladder.

  2. Spatial relationships between an introduced snapper and native goatfishes on Hawaiian reefs

    USGS Publications Warehouse

    Schumacher, B.D.; Parrish, J.D.

    2005-01-01

    It has been suggested that the introduced blueline snapper (Lutjanus kasmira, Family: Lutjanidae) may adversely affect populations of native fishery species in Hawai'i through competition for spatial or dietary resources, or through predation on young fish. We studied the habitat use patterns of L. kasmira and several native reef fish species using direct observation by SCUBA divers. Habitat use patterns of the yellowtail goatfish (Mulloidichthys vanicolensis, Family: Mullidae) were most similar to those of L. kasmira. Both species were primarily found low in the water column and were closely associated with areas of vertical relief. Individual M. vanicolensis were found higher in the water column when L. kasmirawere present, but L. kasmira were not similarly affected by M. vanicolensis. This finding suggests asymmetrical competition for shelter, in which the dominant L. kasmira displaces M. vanicolensis farther into the water column. This displacement from the protection of the reef could increase the vulnerability of M. vanicolensisto predators and fishers. ?? Springer 2005.

  3. Cucullanid nematodes (Nematoda: Cucullanidae) from deep-sea marine fishes off New Caledonia, including Dichelyne etelidis n. sp.

    PubMed

    Moravec, František; Justine, Jean-Lou

    2011-02-01

    Three nematode species of the family Cucullanidae, intestinal parasites of marine perciform fishes, are reported from off New Caledonia: Cucullanus bourdini Petter & Le Bel, 1992 from the crimson jobfish Pristipomoides filamentosus (Valenciennes) and the goldflag jobfish Pristipomoides auricilla (Jordan, Evermann & Tanaka) (new host record) (both Lutjanidae); Dichelyne etelidis n. sp. from the deep-water red snapper Etelis carbunculus Cuvier (type-host) and the deep-water longtail red snapper Etelis coruscans Valenciennes (both Lutjanidae); and Dichelyne sp. (only one female) from the trumpet emperor Lethrinus miniatus (Forster) (Lethrinidae). Detailed light and electron microscopical studies revealed in C. bourdini some taxonomically important, previously unreported features, such as the location of the excretory pore, nature of the vulva and the size of fully-developed eggs. The new species, D. etelidis, is characterised mainly by the length of the spicules (462-748 μm), a single intestinal caecum, the location of the deirids and excretory pore, the arrangement of the genital papillae and the host group.

  4. The complete mitochondrial genome of Channa marulius (Perciformes: Channidae: Channa).

    PubMed

    Cui, Jun; Lashari, Punhal; Zhang, Songhao; Wang, Kai; Xu, Jian; Laghari, Muhammad Younis; Mahboob, Shahid; Al-Ghanim, Khalid A; Zhang, Yan; Xu, Peng

    2016-01-01

    The traditional polymerase chain reaction method was employed to obtain the complete mitochondrial genome of Channa marulius from Pakistan. The mitogenome was determined to be 16,569 bp in length. It contains 13 protein-coding genes, 2 rRNAs and 22 tRNAs. This is the first report on the complete mitogenome sequence of C. marulius.

  5. The complete mitochondrial genome of the Drepane punctata (Perciformes: Drepanidae).

    PubMed

    Xie, Junfeng; Xie, Zhenzhen; Peng, Chen; Xu, Wen; Wang, Qing; Chen, Huapu; Li, Shuisheng; Zhang, Yong; Lin, Haoran

    2016-05-01

    The complete mitochondrial genome of the Drepane punctata was presented in our study. The mitochondrial genome is 16,397 bp long and consists of 13 protein-coding genes, two rRNA genes, 16 tRNA genes and a control region. The gene order and composition of D. punctata mitochondrial genome was different from that of most other vertebrates. The nucleotide compositions of the light strand are 24.56% of A, 16.02% of C, 27.81% of T and 31.61% of G. With the exception of the NADH dehydrogenase subunit 6 (ND6) and five tRNA genes, all other mitochondrial genes are encoded on the heavy strand.

  6. The complete mitochondrial genome sequence of Coreoperca whiteheadi (Perciformes: Serranidae).

    PubMed

    Lv, Liyuan; Tian, Changxu; Liang, Xufang; Yuan, Yongchao; Zhao, Cheng; Song, Yi

    2016-01-01

    In this paper, the complete mitochondrial DNA (mtDNA) sequence of Coreoperca whiteheadi was determined. The complete mtDNA genome sequence of C. whiteheadi is 16,483 bp in length. It consists of 13 protein-coding genes, 22 transfer RNA genes, 2 rRNA genes and 2 non-coding regions. Overall base composition of mitogenome is estimated to be 28.30% for A, 29.33% for C, 16.06% for G and 26.32% for T, respectively, with a high A + T content (54.62%). The complete mitogenome of the C. whiteheadi could contribute to basic researches on population history, molecular systematics and phylogeography. It is also helpful to the reasonable utilization and development of rational management strategies for C. whiteheadi resource.

  7. Molecular identification of Bigeyes (Perciformes, Priacanthidae) from Indian waters.

    PubMed

    K K, Bineesh; A, Gopalakrishnan; J K, Jena; V S, Basheer; C, Mohitha; N, Vineesh; M, Joselet; Pillai, N G K

    2016-11-01

    Thirty-five individuals of six priacanthid fish species were sampled from different localities along the coast of India covering the Arabian Sea and Bay of Bengal. The partial sequence of 16S rRNA and cytochrome oxidase subunit I (COI) genes were analyzed for species identification and phylogenetic relationship among the Indian priacanthids (Priacanthus hamrur, P. prolixus, P. blochii, P. sagittarius, Cookeolus japonicus, and Pristigenys refulgens). The intraspecies genetic distance ranged from 0.000 to 0.002, while distances varied from 0.008 to 0.157 interspecies based on 16S sequences. Using COI data analysis, the intraspecies genetic distance ranged from 0.000 to 0.005, while interspecies distances varied from 0.009 to 0.108. Several sequences labeled Priacanthus hamrur in GenBank are shown to be P. prolixus. We also observed cryptic speciation in Heteropriacanthus cruentatus. Partial sequences of 16S rRNA and COI genes provided phylogenetic information to distinguish thirteen species of priacanthids, indicating the usefulness of molecular markers in species identification.

  8. [Reproductive behavior of Opistognathus rosenblatti (Perciformes: Opistognathidae) in captivity].

    PubMed

    Contreras, Mauricio; Anguas, Benjamín; González, Pedro G; Martínez, Rodolfo E

    2012-09-01

    The Blue Spotted Jawfish O. rosenblatti, is an endemic species from the Gulf of California, included in the local list of protected species. With few biological reports, this species is appreciated in the aquarium industry due to its coloration and digging behaviour, and has a considerable value. With the aim to generate valuable biological information, eight fishes were caught at Loreto Natural Marine Protected Area. Captured fishes were juveniles, and just three of them were kept in an aquarium conditioned with gravel, pieces of shells and coral as substrata. Temperature and photoperiod conditions were stable, and they were supplied with a variety of live and inert feeds. Fishes reached maturity in eight months, according to literature reports, displaying mature male courtship coloration and upward movements in the water column. Fishes spawned several times over more than two years in captivity. Presences of egg masses, or some evidence of egg shell or larvae, were registered in 50 occasions. Male took care of eggs in his shelter, but never in his mouth. Egg masses had an average of 3 592 eggs, with a hatching rate close to 99%. Eggs were apparently rounded having three diameter measurements with significant differences (1.17mm mean higher diameter, SD=0.054; 1.13mm mean lower diameter, 0.058; 0.99mm mean height, 0.045; n=125). Most of corion eggs had four, rarely six filaments; with a single oil drop (0.30mm mean diameter, 0.021, n=59). Incubation lasted 10.4 days (9-14), depending on water temperature (21.0-25.3 degrees C). Egg hatching occurred after darkness, emerging newly hatched larvae of 4.51mm mean notochord length (0.082, n=30), with reserves exhausted, eyes pigmented and mouth opened, ready to eat. This study represents the first report on this species courtship displaying, spawning and some basic characteristics of eggs masses and larvae in captivity. Also, their flexibility and adaptability of individual behaviour to particular environment conditions, lead to consider worth their commercial culture, as already occur with other species of this family.

  9. Cryptic Chromosomal Diversity in the Complex "Geophagus" brasiliensis (Perciformes, Cichlidae).

    PubMed

    Oliveira, Isabel Araújo; Argolo, Leandro Araújo; Bitencourt, Jamille de Araújo; Diniz, Débora; Vicari, Marcelo Ricardo; Affonso, Paulo Roberto Antunes de Mello

    2016-02-01

    Among American cichlids, Geophagus stands out as a species-rich genus widespread over neotropical region. Despite their diversity and confusing taxonomy, only few and basic chromosomal reports are available in populations/species along Atlantic coast, hindering our understanding about evolutionary trends in this genus. Therefore, detailed chromosomal studies were performed in "Geophagus" brasiliensis complex from coastal rivers in northeastern Brazil, totalizing seven populations of Geophagus brasiliensis and one of Geophagus itapicuruensis. All samples shared a diploid number (2n) of 48 divided into 2 submetacentric and 46 subtelocentric/acrocentric chromosomes with (peri)centromeric heterochromatin, hypothesized as a symplesiomorphy for Geophagini. Although G. itapicuruensis and two populations of G. brasiliensis presented single NORs on short arms, multiple GC-rich rDNA sites were observed in the remaining G. brasiliensis samples, ranging from three to six NOR-bearing chromosomes. Inversely, 5S rDNA sites were invariably located at interstitial region on a st/a pair, nonsyntenic to NORs. A compilation of the data set in Geophagus shows that their chromosomal evolution has been driven by pericentric inversions and microstructural changes. Besides, the divergence found in northeastern Brazil places this region as a biodiversity hotspot. A taxonomic revision in the complex "Geophagus" brasiliensis is recommended with the support of cytogenetic analyses.

  10. Mitochondrial genome of the Mackerel scad Decapterus macarellus (Perciformes: Carangidae).

    PubMed

    Zou, Keshu; Chen, Zuozhi; Zhang, Peng; Li, Min

    2016-05-01

    The complete mitochondrial genome sequence was determined for the Mackerel scad Decapterus macarellus, one species of the economically important fish in Carangidae. The entire sequence of the genome was 16,544 bp in length, including the typical structure of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 1 non-coding control region. Overall base compositions of the sequence were 27.3% of A, 30.4% of C, 25.3% of T and 17.0% of G, showing an obvious anti-G bias commonly observed in teleosts. The mitogenome of Decapterus macarellus had a quite high-sequence similarity (92.5%) with D. macrosoma, which was morphologically close to D. macarellus. The complete mitogenome sequence data of D. macarellus could provide useful information for taxonomic and phylogenetics studies.

  11. High Connectivity in the Deepwater Snapper Pristipomoides filamentosus (Lutjanidae) across the Indo-Pacific with Isolation of the Hawaiian Archipelago

    PubMed Central

    Gaither, Michelle R.; Jones, Shelley A.; Kelley, Christopher; Newman, Stephen J.; Sorenson, Laurie; Bowen, Brian W.

    2011-01-01

    In the tropical Indo-Pacific, most phylogeographic studies have focused on the shallow-water taxa that inhabit reefs to approximately 30 m depth. Little is known about the large predatory fishes, primarily snappers (subfamily Etelinae) and groupers (subfamily Epinephelinae) that occur at 100–400 m. These long-lived, slow-growing species support fisheries across the Indo-Pacific, yet no comprehensive genetic surveys within this group have been conducted. Here we contribute the first range-wide survey of a deepwater Indo-Pacific snapper, Pristipomoides filamentosus, with special focus on Hawai'i. We applied mtDNA cytochrome b and 11 microsatellite loci to 26 samples (N = 1,222) collected across 17,000 km from Hawai'i to the western Indian Ocean. Results indicate that P. filamentosus is a highly dispersive species with low but significant population structure (mtDNA ΦST = 0.029, microsatellite FST = 0.029) due entirely to the isolation of Hawai'i. No population structure was detected across 14,000 km of the Indo-Pacific from Tonga in the Central Pacific to the Seychelles in the western Indian Ocean, a pattern rarely observed in reef species. Despite a long pelagic phase (60–180 days), interisland dispersal as adults, and extensive gene flow across the Indo-Pacific, P. filamentosus is unable to maintain population connectivity with Hawai'i. Coalescent analyses indicate that P. filamentosus may have colonized Hawai'i 26 K–52 K y ago against prevailing currents, with dispersal away from Hawai'i dominating migration estimates. P. filamentosus harbors low genetic diversity in Hawai'i, a common pattern in marine fishes, and our data indicate a single archipelago-wide stock. However, like the Hawaiian Grouper, Hyporthodus quernus, this snapper had several significant pairwise comparisons (FST) clustered around the middle of the archipelago (St. Rogatien, Brooks Banks, Gardner) indicating that this region may be isolated or (more likely) receives input from Johnston Atoll to the south. PMID:22216141

  12. Phylogeographic analyses of submesophotic snappers Etelis coruscans and Etelis "marshi" (family Lutjanidae) reveal concordant genetic structure across the Hawaiian Archipelago.

    PubMed

    Andrews, Kimberly R; Moriwake, Virginia N; Wilcox, Christie; Grau, E Gordon; Kelley, Christopher; Pyle, Richard L; Bowen, Brian W

    2014-01-01

    The Hawaiian Archipelago has become a natural laboratory for understanding genetic connectivity in marine organisms as a result of the large number of population genetics studies that have been conducted across this island chain for a wide taxonomic range of organisms. However, population genetic studies have been conducted for only two species occurring in the mesophotic or submesophotic zones (30+m) in this archipelago. To gain a greater understanding of genetic connectivity in these deepwater habitats, we investigated the genetic structure of two submesophotic fish species (occurring ∼200-360 m) in this archipelago. We surveyed 16 locations across the archipelago for submesophotic snappers Etelis coruscans (N = 787) and E. "marshi" (formerly E. carbunculus; N = 770) with 436-490 bp of mtDNA cytochrome b and 10-11 microsatellite loci. Phylogeographic analyses reveal no geographic structuring of mtDNA lineages and recent coalescence times that are typical of shallow reef fauna. Population genetic analyses reveal no overall structure across most of the archipelago, a pattern also typical of dispersive shallow fishes. However some sites in the mid-archipelago (Raita Bank to French Frigate Shoals) had significant population differentiation. This pattern of no structure between ends of the Hawaiian range, and significant structure in the middle, was previously observed in a submesophotic snapper (Pristipomoides filamentosus) and a submesophotic grouper (Hyporthodus quernus). Three of these four species also have elevated genetic diversity in the mid-archipelago. Biophysical larval dispersal models from previous studies indicate that this elevated diversity may result from larval supplement from Johnston Atoll, ∼800 km southwest of Hawaii. In this case the boundaries of stocks for fishery management cannot be defined simply in terms of geography, and fishery management in Hawaii may need to incorporate external larval supply into management plans.

  13. Cucullanin nematodes from coral reef fishes of French Polynesia, with a description of Cucullanus faliexae n. sp. (Nematoda: Chitwoodchabaudiidae).

    PubMed

    Morand, S; Rigby, M C

    1998-12-01

    Cucullanus faliexae n. sp. is described from the moray eel Gymnothorax javanicus (Muraenidae) collected in Rangiroa (Tuamotu Islands, French Polynesia) in the South Pacific and is reported from the same host collected in Moorea (Society Islands, French Polynesia). This nematode is distinguished from its congeners by its protrubant anus and the presence of a single pair of subdorsal postanal papillae. Cucullanus faliexae resembles C. laurotravassosi from which it is distinguished by the farther anterior position of the first pair of male caudal preanal papillae and by the greater size of its spicules. Cucullanus bourdini Petter and Le Bel, 1992 is reported from the coral reef fishes Balistapus undulatus (Balistidae), Lutjanus gibbus (Lutjanidae), and Myripristis kuntee (Holocentridae) from French Polynesia. Additionally, Cucullanus sp. is reported from the coral reef fish Sufflamen bursa (Balistidae) in Moorea and Rangiroa. Given that C. faliexae and C. bourdini have been found in widely disparate locations between which their definitive hosts cannot migrate, it would seem likely that the life cycles of these worms include an intermediate or transport host that has a greater capability for dispersal.

  14. First occurrence of Argathona macronema (Bleeker, 1857) (Isopoda: Cirolanidae) on coral reef fishes along the Tuticorin coastal water, Gulf of Mannar.

    PubMed

    Chelladurai, Gurusamy; Mohanraj, Jayaraj; Jayanthi, Govinda Rajalu

    2017-03-01

    Isopod Parasites are considered as a major threat to the fishing industry, due to rigorous disease outbreaks. The parasites from the two economically important reef fish family Lutjanidae and Serranidae are analyzed i.e. Epinephelus malabaricus and Lutjanus rivulatus. The samples are obtained from the landings of Trawl net from Gulf of Mannar region of South east coast of India. They are severely infested by the isopod parasite Agrathona macronema in the Pectoral fins and ventral region of the body. This species is found to be the new record in the Gulf of Mannar region and deposited in the Marine laboratory of Kamaraj College. Length and weight of the host and parasites are measured. The parasites infested on 13 specimens of E. malabricus out of 8 and 17 out of 12 species of L. rivulatus. The prevalence of parasite on the grouper and snapper fish (61.53 and 70.58 %) and mean intensity (2.62 and 2.58 %) respectively. The prevalence was maximum in L. rivulatus and minimum in E. malbaricus may be to the host specificity. Further studies are needed to know the life cycle of the parasite, host preference, disease outbreak and its role in the coral reef ecosystem.

  15. Survey of ciguatera at Enewetak and Bikini, Marshall Islands, with notes on the systematics and food habits of ciguatoxic fishes

    SciTech Connect

    Randall, J.E.

    1980-04-01

    A total of 551 specimens of 48 species of potentially ciguatoxic fishes from Enewetak and 256 specimens of 23 species from Bikini, Marshall Islands, were tested for ciguatoxin by feeding liver or liver and viscera from these fishes to mongooses at 10% body weight (except for sharks, when only muscle tissue was used). The fishes are representatives of the following families: Orectolobidae, Carcharhinidae, Dasyatidae, Muraenidae, Holocentridae, Sphyraenidae, Mugilidae, Serranidae, Lutjanidae, Lethrinidae, Carangidae, Scombridae, Labridae, Scaridae, Acanthuridae, and Balistidae. The species selected were all ones for which toxicity can be expected, including the worst offenders from reports of ciguatera throughout Oceania; only moderate to large-sized adults were tested. In all, 37.3% of the fishes from Enewetak and 19.7% from Bikini gave a positive reaction for ciguatoxin. Because liver and other viscera are more toxic than muscle, the percentage of positive reactions at the level which might cause illness in humans eating only the flesh of these fishes collectively would drop to 16.2 for Enewetak and 1.4 for Bikini. This level of toxicity is not regarded as high for Pacific islands, in general. Because ciguatoxin is acquired through feeding, the food habits of these fishes were investigated. Most of the highly toxic species, including seven of the eight causing severe illness or death in the test animals (Lycodontis javanicus, Cephalopholis argus, Epinephelus hoedtii, E. microdon, Plectropomus leopardus, Aprion virescens, and Lutjanus bohar) are primarily piscivorous.

  16. Protozoal and epitheliocystis-like infections in the introduced bluestripe snapper (Lutjanus kasmira) in Hawaii

    USGS Publications Warehouse

    Work, Thierry M.; Rameyer, Robert; Takata, Geraldine; Kent, Michael L.

    2003-01-01

    The bluestripe snapper, or taape, was introduced into Hawaii in the 1950s and has since become very abundant throughout the archipelago. As part of a health survey of reef fish in Hawaii, we necropsied 120 taape collected from various coastal areas south of Oahu and examined fish histology for extraintestinal organisms. Forty-seven percent of taape were infected with an apicomplexan protozoan compatible with a coccidian. Infection was evident mainly in the spleen and, less commonly, the kidney. Prevalence of this coccidian increased with size of fish, and we saw no significant pathology associated with the organism. Twenty-six percent of taape were also infected with an epitheliocystis-like organism that occurred mainly in the kidney and, less commonly, the spleen. In contrast to the coccidian, fish mounted a notable inflammatory response to the epitheliocystis-like organism, and this inflammation appeared to increase in severity with age. Prevalence of the epitheliocystis-like organism infection increased with age, but infection was not seen in fish greater than 26.5 cm fork length. The high prevalence of coccidial infection in introduced taape prompts the concern that these organisms, along with the epitheliocystis-like organism, have the potential to be transmitted to native reef fish. Given the impact of other introduced microbial organisms on native Hawaiian fauna, there is a clear need to assess whether protozoa and bacteria are endemic to Hawaii, and whether they negatively impact native reef fish that closely associate with taape.

  17. Age, growth, and natural mortality of schoolmaster (Lutjanus apodus) from the southeastern United States

    PubMed Central

    Burton, Michael L.; Myers, Amanda R.

    2016-01-01

    Ages of schoolmaster (n = 136) from the southeastern Florida coast from 1981–2015 were determined using sectioned sagittal otoliths. Opaque zones were annular, forming March–July (peaking in May–June). Schoolmaster ranged in age from 1–42 years; the largest fish measured 505 mm total length (TL) and was 19 years old. The oldest fish measured 440 mm TL. Estimated body size relationships for schoolmaster were: W = 9.26 × 10−6 TL3.11 (n = 256, r2 = 0.95); W = 2.13 × 10−5 FL2.99 (n = 161, r2 = 0.95); TL = 1.03 FL + 10.36 (n = 143, r2 = 0.99); and FL = 0.96 TL − 8.41 (n = 143, r2 = 0.99), where W = whole weight in g, FL = fork length in mm, and TL in mm. The fitted von Bertalanffy growth equation was: Lt = 482 (1 − e−0.12(t+2.79)) (n = 136). Based on published life history relationships, a point estimate of natural mortality for schoolmaster was M = 0.10, while age-specific estimates of M ranged from 1.57–0.18 for ages 1–42. PMID:27761332

  18. Protozoal and epitheliocystis-like infections in the introduced bluestripe snapper (Lutjanus kasmira) in Hawaii

    USGS Publications Warehouse

    Work, T.M.; Rameyer, R.A.; Takata, G.; Kent, M.L.

    2003-01-01

    The bluestripe snapper, or taape, was introduced into Hawaii in the 1950s and has since become very abundant throughout the archipelago. As part of a health survey of reef fish in Hawaii, we necropsied 120 taape collected from various coastal areas south of Oahu and examined fish histology for extraintestinal organisms. Forty-seven percent of taape were infected with an apicomplexan protozoan compatible with a coccidian. Infection was evident mainly in the spleen and, less commonly, the kidney. Prevalence of this coccidian increased with size of fish, and we saw no significant pathology associated with the organism. Twenty-six percent of taape were also infected with an epitheliocystis-like organism that occurred mainly in the kidney and, less commonly, the spleen. In contrast to the coccidian, fish mounted a notable inflammatory response to the epitheliocystis-like organism, and this inflammation appeared to increase in severity with age. Prevalence of the epitheliocystis-like organism infection increased with age, but infection was not seen in fish greater than 26.5 cm fork length. The high prevalence of coccidial infection in introduced taape prompts the concern that these organisms, along with the epitheliocystis-like organism, have the potential to be transmitted to native reef fish. Given the impact of other introduced microbial organisms on native Hawaiian fauna, there is a clear need to assess whether protozoa and bacteria are endemic to Hawaii, and whether they negatively impact native reef fish that closely associate with taape.

  19. Phylogeographic Analyses of Submesophotic Snappers Etelis coruscans and Etelis “marshi” (Family Lutjanidae) Reveal Concordant Genetic Structure across the Hawaiian Archipelago

    PubMed Central

    Andrews, Kimberly R.; Moriwake, Virginia N.; Wilcox, Christie; Grau, E. Gordon; Kelley, Christopher; Pyle, Richard L.; Bowen, Brian W.

    2014-01-01

    The Hawaiian Archipelago has become a natural laboratory for understanding genetic connectivity in marine organisms as a result of the large number of population genetics studies that have been conducted across this island chain for a wide taxonomic range of organisms. However, population genetic studies have been conducted for only two species occurring in the mesophotic or submesophotic zones (30+m) in this archipelago. To gain a greater understanding of genetic connectivity in these deepwater habitats, we investigated the genetic structure of two submesophotic fish species (occurring ∼200–360 m) in this archipelago. We surveyed 16 locations across the archipelago for submesophotic snappers Etelis coruscans (N = 787) and E. “marshi” (formerly E. carbunculus; N = 770) with 436–490 bp of mtDNA cytochrome b and 10–11 microsatellite loci. Phylogeographic analyses reveal no geographic structuring of mtDNA lineages and recent coalescence times that are typical of shallow reef fauna. Population genetic analyses reveal no overall structure across most of the archipelago, a pattern also typical of dispersive shallow fishes. However some sites in the mid-archipelago (Raita Bank to French Frigate Shoals) had significant population differentiation. This pattern of no structure between ends of the Hawaiian range, and significant structure in the middle, was previously observed in a submesophotic snapper (Pristipomoides filamentosus) and a submesophotic grouper (Hyporthodus quernus). Three of these four species also have elevated genetic diversity in the mid-archipelago. Biophysical larval dispersal models from previous studies indicate that this elevated diversity may result from larval supplement from Johnston Atoll, ∼800 km southwest of Hawaii. In this case the boundaries of stocks for fishery management cannot be defined simply in terms of geography, and fishery management in Hawaii may need to incorporate external larval supply into management plans. PMID:24722193

  20. The acid solvent experimental studies on gelatin producing by utilizing snapper fishes scales waste (Lutjanus camphecanus sp.)

    NASA Astrophysics Data System (ADS)

    Faradiella, Hanandyta; Ningsih, Putri Dewi Fatwa; Triastuti, Warlinda Eka

    2017-03-01

    Had done research processing scales red snapper (Lutjanjus campechanus sp.) into gelatin by process of maseration a solution of citric acid, acetate acid, hydrochloric acid, and phosphoric acid an then continued with hydrolysis process. Concentration of solvent used 5% (% v/v) for 3 and 6 days. Gelatin produces then were analyzed fourier transform infrared spectroscopy (FTIR), proximate analysis include yield and the water content, analysis of physical-chemist properties include analysis viscosity and pH, organoleptic test (color, texture, odor). Analyst ftir results showed the presence of a cluster functions 0-H, C-H, C=0, N-H and C-C aromatis similar to those of spectra that are found on commercial gelatin. Gelatin best results obtained maseration on with phosphoricacid 5% viscosity of gelatin 3,91 cP, the water content 2,569%, pH 6,98 and yield 11,7448%.

  1. In vitro immunostimulatory potential of fungal β-glucans in pacific red snapper (Lutjanus peru) cells.

    PubMed

    Cárdenas-Reyna, Tomás; Angulo, Carlos; Guluarte, Crystal; Hori-Oshima, Sawako; Reyes-Becerril, Martha

    2017-12-01

    This study attempts to describe the immunostimulatory effects of three fungal glucans on innate immunity responses in an in vitro assays using Pacific red snapper leukocytes. First, the yield glucans obtained was higher in Aspergillus niger, follow by Aspergillus ochraceus and Alternaria botrytis (40, 20 and 10%, respectively). Structural characterization of these fungal glucans by proton nuclear magnetic resonance (NMR) indicated structures containing (1-6)-branched (1-3)-β-D-glucan. The immunostimulatory activity of fungal glucans were assessed in head-kidney leukocytes at 24 h using colorimetric assays and molecular gene expression. In addition, the response against bacterial infection using Aeromonas hydrophila was evaluated by flow cytometry with annexin V/propidium iodide. Leukocytes responded positively to fungal glucans where the viability was higher than 80%. Interestingly, A. niger β-glucans enhanced the phagocytic ability and capacity in head-kidney leukocytes. Immunological assays reveled an increased in nitric oxide production, myeloperoxidase, superoxide dismutase and catalase activities, in fish stimulated with A. niger β-glucans. Induction of cytokines (IL-1β, TNF-α, IL-6, IL-8 and IL-12) were more pronounced in A. niger β-glucans leukocytes stimulated compared to other group. Finally, flow cytometry assay showed that A. botrytis and A. niger β-glucans were able to inhibit apoptosis caused by Aeromonas hydrophila in the Pacific red snapper leukocytes indicating an immunostimulant potent response by fungi derived-glucans. These results strongly support the idea that fungal β-glucans can stimulate the immune mechanism in head-kidney leukocytes and that Aspergillus niger β-glucan possess immunostimulatory properties cell increasing viability, and reducing necrotic cell death caused by Aeromonas hydrophila. Copyright © 2017 Elsevier Ltd. All rights reserved.

  2. Condition of larval red snapper (Lutjanus campechanus) relative to environmental variability and the Deepwater Horizon oil spill

    NASA Astrophysics Data System (ADS)

    Hernandez, F. J., Jr.; Filbrun, J. E.; Fang, J.; Ransom, J. T.

    2016-09-01

    The Deepwater Horizon oil spill (DWHOS) spatially and temporally overlapped with the spawning of many fish species, including Red Snapper, one of the most economically important reef fish in the Gulf of Mexico. To investigate potential impacts of the DWHOS on larval Red Snapper, data from a long-term ichthyoplankton survey off the coast of Alabama were used to examine: (1) larval abundances among pre-impact (2007-2009), impact (2010), and post-impact (2011, 2013) periods; (2) proxies for larval condition (size-adjusted morphometric relationships and dry weight) among the same periods; and (3) the effects of background environmental variation on larval condition. We found that larval Red Snapper were in poorer body condition during 2010, 2011, and 2013 as compared to the 2007-2009 period, a trend that was strongly (and negatively) related to variation in Mobile Bay freshwater discharge. However, larvae collected during and after 2010 were in relatively poor condition even after accounting for variation in freshwater discharge and other environmental variables. By contrast, no differences in larval abundance were detected during these survey years. Taken together, larval supply did not change relative to the timing of the DWHOS, but larval condition was negatively impacted. Even small changes in condition can affect larval survival, so these trends may have consequences for recruitment of larvae to juvenile and adult life stages.

  3. [The System of the Suborder Zoarcoidei (Pisces, Perciformes) as Inferred from Molecular Genetic Data].

    PubMed

    Radchenko, O A

    2015-11-01

    Based on an analysis of sequence variation in mitochondrial and nuclear markers, the levels of divergence, relationships, and system of the suborder Zoarcoidei was defined. It was demonstrated that DNA lineages of the families Bathymasteridae and Cebidichthyidae were positioned at the bottom ofthe suborder phylogenetic tree. The family Zoarcidae is a monophyletic group, the youngest in the evolutionary terms. Zoarcidae, Anarhichadidae, Neozorcidae, and Eulophiidae form a group of related families. The family Stichaeidae is heterogeneous and has a polyphyletic origin; within this family, the subfamilies Chirolophinae, Alectgiinae, Xiphisterinae, and Stichaeinae are sister taxa. The subfamilies Opisthocentrinae and Lumpeninae are isolated from Stichaedae; Opisthocentrinae is closely associated with the families Pholidae and Ptilichthyidae, and Lumpeninae is closely associated with Zaproridae and Cryptacanthodidae. It is suggested that the rank of subfamilies Opisthocentrinae and Lumpeninae should be raised.

  4. Two new species of Coryogalops (Perciformes: Gobiidae) from the Red Sea .

    PubMed

    Kovačić, Marcelo; Bogorodsky, Sergey V; Mal, Ahmad O

    2014-11-10

    Two new species of the gobiid genus Coryogalops, C. guttatus sp. nov. and C. pseudomonospilus sp. nov., are described from the Red Sea. Coryogalops guttatus sp. nov. is distinguished from its congeners by having dorsal-fin rays VI + I,12; anal-fin rays I,10-11; pectoral-fin rays 17, three upper rays with free tips; pelvic fins I,5, joined to form a disc, weakly emarginate, with pelvic frenum reduced; longitudinal scale series 33-35; transverse scale rows 9-10; circumpeduncular scales 10-11; seven transverse suborbital rows of sensory papillae on cheek; no tentacle above upper eye margin; anterior nostril tubular, with triangular lappet extending from posterior rim, posterior nostril slightly raised; dark orange spots on many of the scales below body midline form a longitudinal pattern, each spot covering one scale; two distinct dark spots behind edge of preopercle, and similar pair of spots at pectoral-fin base. Coryogalops pseudomonospilus sp. nov. is distinguished from congeners by having dorsal-fin rays VI + I,11; anal-fin rays I,10; pectoral-fin rays 16-17, two upper rays with free tips; pelvic fins I,5, joined to form a disc, moderately emarginate, with pelvic frenum well-developed; longitudinal scale series 33-35; transverse scales rows 9; circumpeduncular scales 12; seven transverse suborbital rows of sensory papillae on cheek; no tentacle above upper eye margin; anterior nostril tubular, without lappet from posterior rim, posterior nostril slightly raised; and irregular dark maroon mark covering lower part of the first three membranes between spines of first dorsal fin. The distribution of species restricted to the western Indian Ocean is discussed, and a key to the species of the genus is provided.

  5. Phylogeny and taxonomy of Petroschmidtia teraoi (Katayama, 1943) (Osteichthyes: Perciformes: Zoarcidae).

    PubMed

    Nazarkin, Mikhail V; Shinohara, Gento; Shirai, Shigeru M

    2014-03-20

    A morphological and genetic reassessment of the phylogeny and taxonomy of the dwarf zoarcid fish Lycodes teraoi Katayama, 1943 indicated that the species, a senior synonym of Lycodes sadoensis Toyoshima & Honma, 1980, should be placed in the genus Petroschmidtia. A redescription of P. teraoi is provided, with remarks on its taxonomy. Numerous specimens revealed a wide distribution of P. teraoi in the Sea of Japan, as well as in the southern Sea of Okhotsk.

  6. Complete mitochondrial genome of the pink clownfish Amphiprion perideraion (Pisces: Perciformes, Pomacentridae).

    PubMed

    Hu, Xueyi; Li, Jianlong; Liu, Min

    2016-01-01

    In this study the complete mitochondrial (mt) genome of the pink clownfish Amphiprion perideraion was obtained by using eight consensus primer pairs with a long PCR technique. The circular mtDNA molecule was 16,579 bp in size and the overall nucleotide composition of the H-strand was 29.37% A, 25.50% T, 15.68% G and 29.45% C, with an A + T bias. The complete mitogenome contained 13 protein-coding genes, 2 rRNAs, 22 tRNAs and a control region, and the gene order was typical of vertebrate mitogenomes. The complete mitochondrial genome of A. perideraion is a representative of the subgenus Phalerebus for mitogenomes database of anemonefishes, which can be used to unveil taxonomic problems and phylogenetic relationships in Amphiprioninae.

  7. Two new fish species of the subfamily Anthiinae (Perciformes, Serranidae) from the Marquesas.

    PubMed

    Williams, Jeffrey T; Delrieu-Trottin, Erwan; Planes, Serge

    2013-01-01

    Two new species of anthiine fishes are described from the Marquesas Islands, French Polynesia. Plectranthias flammeus was found at depths from 20-45 m and is characterized by dorsal rays X, 14 or 15, with spines 1-6 bearing fleshy white tabs at their tips, longest fleshy tab on spine 4; 14 unbranched pectoral rays; lateral line incomplete with 16-17 tubed scales; preopercle with 8-10 small spines along posterior margin and 2 antrorse spines on ventral margin; broad, fiery red-orange streak across lower cheek; head and body with irregularly spaced maroon-ringed yellow blotches on a white background; pair of small dark oblong spots (red with black centers in life) on the bases of the middle rays of the caudal fin. Pseudanthias oumati was found on the outer reef slope of Fatu Hiva at a depth of 50-55 m and is characterized by 3rd dorsal spine elongate and tipped with fleshy yellow filament extending beyond tip of spine; lateral-line scales 43; gill rakers 10 + 28; no papillae on posterior edge of orbit; front of upper lip not thickened (male condition unknown); caudal fin lunate; color of female yellow, all fins yellow with narrow magenta margin (except pectoral fin, which lacks magenta); no stripe from snout to pectoral base; small scales located on basal quarter of soft-dorsal fin from segmented rays 1-12; dorsal profile of head slightly concave.

  8. Review of the harvestfishes, genus Peprilus (Perciformes: Stromateidae),
    of the Atlantic coast of South America.

    PubMed

    Marceniuk, Alexandre P; Caires, Rodrigo; Siccha-Ramirez, Raquel; Oliveira, Claudio

    2016-04-06

    Currently, seven valid species are recognized in the genus Peprilus. Found from United States to Argentina, Peprilus paru has a complex nomenclatural history, with seven junior synonyms, three from North America and four from South America. As there has been no recent research, it remains unclear whether species representatives in the north-south axis represent different populations of a single species or distinct species. By comparison of type specimens as well as a comprehensive collection of non-type specimens, this paper aims to clarify the taxonomic status of the nominal species listed as junior synonyms of Peprilus paru in the Atlantic side of South America. Based on morphological data and DNA barcoding, Peprilus crenulatus Cuvier, 1829 and P. xanthurus (Quoy & Gaimard, 1825) are resurrected, while Rhombus argentipinnis Cuvier, 1833 and Rhombus orbicularis Guichenot, 1866, are considered to be junior synonyms of P. crenulatus.

  9. First report of the black gemfish Nesiarchus nasutus (Perciformes: Gempylidae) in Argentinean waters.

    PubMed

    Spath, M C; Delpiani, G; Brunetti, N E; Sakai, M; Figueroa, D E

    2015-05-01

    The gempylid Nesiarchus nasutus is reported for the first time from Argentinean waters, the southernmost occurrence of the species in the Southwestern Atlantic. This suggests that the fluctuating environmental characteristics of the area would be proper for the presence of tropical and subtropical species.

  10. A new species of Larimichthys from Terengganu, east coast of Peninsular Malaysia (Perciformes: Sciaenidae).

    PubMed

    Seah, Ying Giat; Hanafi, Norhafiz; Mazlan, Abd Ghaffar; Chao, Ning Labbish

    2015-05-08

    A new species of Larimichthys from Terengganu, east coast of Peninsular Malaysia is described from specimens collected from the fish landing port at Pulau Kambing, Kuala Terengganu. Larimichthys terengganui can be readily distinguished from other species of the genus by having an equally short pair of ventral limbs at the end of the gas bladder appendages, which do not extend lateral-ventrally to the lower half of the body wall, and fewer dorsal soft rays (29-32 vs. 31-36) and vertebrae (24 vs. 25-28). Larimichthys terengganui can be distinguished from L. polyactis and L. crocea by having a gill raker at the angle of first gill arch shorter than the gill filament. Furthermore, the second anal spine in L. terengganui is equal or slightly shorter than eye diameter (vs. shorter in L. polyactis); L. terengganui has 8-9 anal soft rays (vs. only 7 in L. pamoides). Snout length of L. terengganui is greater than eye diameter, whereas in L. crocea the snout is shorter than eye diameter. A key to species of Larimichthys is provided. All obtained specimens of the species were recorded from Terengganu waters, east coast of Peninsular Malaysia.

  11. Assessment of the dolphinfish Coryphaena hippurus (Perciformes: Coryphaenidae) fishery in Pacific Panama.

    PubMed

    Guzman, Héctor M; Díaz-Ferguson, Edgardo; Vega, Angel J; Robles, Yolany A

    2015-09-01

    The dolphinfish (Coryphaena hippunis) is a highly migratory pelagic species commercially exploited by industrial, artisanal and recreational fisheries in tropical and subtropical areas of the world's oceans. Herein, we evaluated the dolphinfish industrial fishery in Pacific Panama for the first time generating a growth model and examining fluctuations in annual total catch and in catch per unit effort (CPUE) over a four-year period (2006-2009). Annual and monthly catch values and biological parameters of 14913 individuals were obtained onboard industrial vessels, landing sites and records from processing plants. Size frequency for industrial vessels showed a normal distribution between 353 and 1,715 mm (average, 1010.85 mm; n = 10,459). Fish weight averaged 4.94 kg (SD). Sex ratio was slightly biased toward females. More than 90% of the analyzed fish were sexually mature. The length-weight relationship was positive and significant, reflecting allometric growth. Growth parameters using the von Bertalanffy equation revealed a growth efficiency of θ = 4.61, which is within the reported range for Coryphaena hippurus (3.95-4.70). The largest fish were between age classes 2 and 3 (700 - 1400 mm). Total catch per year and catch per unit effort (CPUE) per year fluctuated, with the highest values recorded between 2008 and 2009. Catch values reported herein are preliminary and appear to be below those recorded elsewhere in the region and cautiously may represent an indicator of sustainable use of this marine resource even considering the absence of management actions in Panama.

  12. Complete mitochondrial genome of the sixbar grouper Epinephelus sexfasciatus (Perciformes: Epinephelidae).

    PubMed

    Du, Fei-Yan; Ye, Le; Wang, Xue-Hui

    2015-06-01

    In this study, we presented the complete mitogenome for the sixbar grouper Epinephelus sexfasciatus. The complete mitogenome of E. sexfasciatus is 16,786 bp in length with the typical mitochondrial gene order and composition in vertebrates. Overall base composition was 28.40% A, 27.93% C, 27.21% T and 16.45% G. The COI gene used GTG and the ATP6 gene used CTG as the start codon. The tRNA-Ser2 lost the dihydrouridine arm and replaced with a simple loop. Both the maximum likelihood (ML) and Bayesian inference (BI) methods yielded the same tree topology using available mitogenomes of the genus Epinephelus. Epinephelus sexfasciatus was nested to E. akaara. E. awoara and E. fasciatomaculosus, and then combined with E. stictus formed a clade.

  13. Epinephelus moara: a valid species of the family Epinephelidae (Pisces: Perciformes).

    PubMed

    Liu, M; Li, J-L; Ding, S-X; Liu, Z-Q

    2013-05-01

    This study documents the major external and internal morphological differences between Epinephelus bruneus and Epinephelus moara, and analyses the complete mitogenomes of both species. The partial cytochrome oxidase subunit I (coI) sequence divergence between E. bruneus and E. moara is significantly higher than specimens within the same species (P < 0·05). Analyses of gene flow (Nm = 0·02) and genetic differentiation (ϕst = 0·92995, P > 0·05) reveal reproductive isolation between E. bruneus and E. moara. These results support the hypothesis that E. moara is a valid species. Further molecular comparisons between E. bruneus and E. moara obtained in this study and a specimen identified in GenBank as E. bruneus from South Korea reveal that the latter is identical to E. moara rather than to E. bruneus.

  14. Comparative ontogeny of the feeding apparatus of sympatric drums (Perciformes: Sciaenidae) in the Chesapeake Bay.

    PubMed

    Deary, Alison L; Hilton, Eric J

    2016-02-01

    The anatomy of the feeding apparatus in fishes, including both oral and pharyngeal jaw elements, is closely related to the ecology of a species. During ontogeny, the oral and pharyngeal jaws undergo dramatic changes. To better understand how such ontogenetic changes occur and relate to the feeding ecology of a species, ontogenetic series of four closely related members of the family Sciaenidae (Cynoscion nebulosus, Cynoscion regalis, Micropogonias undulatus, and Leiostomus xanthurus) were examined. Sciaenids were selected because as adults they exhibit considerable specialization of the feeding apparatus correlated with differences in foraging habitats. However, it is not clear when during ontogeny the structural specializations of the feeding apparatus develop, and thereby enable early life history stage (ELHS) sciaenids to partition their foraging habitats. A regression tree was recovered from the analysis and three divergences were identified during ontogeny. There are no measurable differences in elements of the feeding apparatus until the first divergence at 8.4 mm head length (HL), which was attributed to differences in average gill filament length on the second ceratobranchial. The second divergence occurred at 14.1 mm HL and was associated with premaxilla length. The final divergence occurred at 19.8 mm HL and was associated with differences in the toothed area of the fifth certatobranchial. These morphological divergences suggest that ELHS sciaenids may be structurally able to partition their foraging habitats as early as 8.4 mm HL.

  15. Complete sequence and characterization of mitochondrial DNA genome of Channa asiatica (Perciformes: Channidae).

    PubMed

    Meng, Yan; Zhang, Yan

    2016-01-01

    The complete nucleotide sequence of Channa asiatica mitochondrial (mtDNA) genome was determined in this study. The genome sequence (GenBank accession number KJ930190) was 16,550 base pairs in length, and the gene content and organization on the mitochondrial genome were similar to the other Channa fishes. The overall base composition of C. asiatica mitogenome is 29.4% A, 26.3% T, 15.3% G, 29.0% C, with a high A + T content of 55.7%. The mitochondrial sequence could provide useful genetic information for studying the molecular identification, population genetics, phylogenetic analysis and conservation genetics.

  16. Chelidoperca stella, a new species of perchlet (Perciformes: Serranidae) from the Andaman Sea, eastern Indian Ocean.

    PubMed

    Matsunuma, Mizuki; Motomura, Hiroyuki

    2016-03-16

    A new species of serranid fish, Chelidoperca stella, is described on the basis of five specimens from the Andaman Sea in the eastern Indian Ocean. The species can be readily distinguished from all valid congeners by having fewer pored lateral-line scales (34 or 35 vs. 40-45 in the latter) and the pelvic fin white with several small yellow spots (vs. spots absent). The species is also characterized by having relatively high counts of small serrae on the posterior margins of the preopercle (33-43 serrae), interopercle (7-10) and subopercle (18-28), despite the small body size (51.7-61.8 mm SL). Although Chelidoperca stella resembles C. margaritifera in having 2.5 scale rows between lateral line and the sixth dorsal-fin spine base, a relatively wider interorbital region, and the interorbital scales not reaching a vertical through the orbit anterior margin, but differs by having the above-mentioned diagnostic characters plus a slightly shorter longest anal-fin soft ray [17.4-18.7 (mean 18.1) % SL vs. 22.6-26.4 (24.4) % SL in C. margaritifera].

  17. Reproductive tactics optimizing the survival of the offspring of Cichlasoma orientale (Perciformes: Cichlidae).

    PubMed

    Spíndola Linhares, Jussiara Candeira; Resende Manna, Luisa; Mazzoni, Rosana; Ferreira Rezende, Carla; Feitosa Silva, José Roberto

    2014-09-01

    High mortality rates have been observed in Teleost during early developmental stages, as well as great variations in reproductive tactics, which are related to adaptations towards environmental conditions and ecological niches for which different species have a specific response. The objective of this study was to describe reproductive tactics related to the survival of Cichlasoma orientale offspring, including aspects of body size, parental care, fecundity, oocyte size and spawning patterns. Samples were performed monthly from August 2011 to July 2013, in lentic and lotic environments at Curu river basin, Brazilian Northeastern semiarid region. Individual behavior (n = 13) was observed underwater for over 50 hours by ad libitum sampling and focal-animal sampling. Collected individuals (males n = 185, females n = 95) were evaluated regarding the standard length, batch fecundity, oocyte size and spawning pattern. In females with mature ovaries, oocyte groups at different developmental stages were observed, these cells were counted and measured, and fecundity was estimated by the gravimetric method. Our results showed that the species displayed biparental care behavior and, on average, males were larger than females. Based on 46 ovaries, the average batch fecundity was 2,052-849 (range: 254-3 389). Standard length and batch fecundity were positively correlated, but no correlation was found between oocyte size and standard length. The maximum diameter observed in the most developed oocytes was 1.8 mm. The observed distribution of oocyte size classes indicated synchronous oocyte development in three groups: pre- vitellogenic, vitellogenic and mature, showing that C. orientale is a multiple spawner. Differences in the amount of oocytes among the three groups were observed, with the most developed group showing the smallest number of oocytes. The combination of low fecundity and large egg size is characteristic of demersal spawners due to a greater environmental stability. Multiple spawning increases chances of survival mainly because of increased fecundity per reproductive season, and the reduced competition among the offspring. We concluded that C. orientale makes a heavy investment in larval survival in detriment of the offspring number. Survival is favored by the large size of oocytes, large yolk reserve, biparental care and multiple spawning pattern.

  18. A new species of Hetereleotris (Perciformes: Gobiidae) from the Red Sea.

    PubMed

    Kovačić, Marcelo; Bogorodsky, Sergey V

    2014-02-12

    A new species of the genus Hetereleotris is described from the Gulf of Aqaba, Red Sea, on the basis of two specimens. Hetereleotris psammophila sp. nov. is unique among the species of the genus Hetereleotris, except for H. diademata, in lacking scales and head pores. The new species differs from the morphologically similar H. diademata in having fewer rays in the second dorsal and anal fins, and in coloration. The habitat preference of the new species for open sand area close to coral reefs in 8-21 m and its nocturnal habits are unusual for species of the genus Hetereleotris.

  19. Wallaceochromis gen. nov, a new chromidotilapiine cichlid genus (Pisces: Perciformes) from West Africa.

    PubMed

    Lamboj, Anton; Trummer, Franziska; Metscher, Brian D

    2016-07-26

    A new genus, Wallaceochromis, is described for the chromidotilapiine cichlids of the Pelvicachromis humilis group. It differs from Pelvicachromis sensu stricto in the number and arrangement of tubular infraorbitals, a straighter and sloping dorsal head profile with a low supraoccipital crest, higher abdominal vertebral counts (14-15 vs. 13-14), and in displaying a pattern of vertical bars on the body in several behavioral situations.

  20. Immune adaptive response induced by Bicotylophora trachinoti (Monogenea: Diclidophoridae) infestation in pompano Trachinotus marginatus (Perciformes: Carangidae).

    PubMed

    Chaves, I S; Luvizzotto-Santos, R; Sampaio, L A N; Bianchini, A; Martínez, P E

    2006-09-01

    Fish have developed protective strategies against monogeneans through immunological responses. In this study, immune adaptive response to parasites was analysed in the pompano Trachinotus marginatus infested by Bicotylophora trachinoti. Hosts were pre-treated with formalin and after 10 days assigned to one of the following experimental treatments: (1) fish infested with remaining eggs of B. trachinoti; (2) fish infested with remaining eggs of B. trachinoti and experimentally re-infested by exposure to T. marginatus heavily infested with B. trachinoti. Samples were collected at 0, 15, and 30 days. Gills were dissected to check the presence of B. trachinoti. Blood was collected for haematological and biochemical assays. Spleen and head-kidney were dissected for phagocytosis assay. The spleen-somatic index was also calculated. Re-infested fish showed a faster and higher parasite infestation than infested ones. The parasite mean abundance at 15 days was 24.86+/-13.32 and 11.67+/-8.57 for re-infested and infested fish, respectively. In both groups, hosts showed an immune adaptive response to parasite infestation that was marked by an increased number of leukocytes. Also, phagocytosis (%) in spleen and head-kidney cells was stimulated after parasite infestation (92.50+/-3.73 and 66.00+/-9.54, respectively), becoming later depressed (77.39+/-6.69 and 53.23+/-9.14, respectively). These results support the hypothesis that monogenean infestation induces a biphasic response of the non-specific defence mechanisms in the pompano T. marginatus. This response is marked by an initial stimulation followed by a later depression of the non-specific defence mechanisms.

  1. Prognathodes basabei, a new species of butterflyfish (Perciformes, Chaetodontidae) from the Hawaiian Archipelago

    PubMed Central

    Pyle, Richard L.; Kosaki, Randall K.

    2016-01-01

    Abstract A new species of the butterflyfish genus Prognathodes is described from specimens collected at a depth of 55–61 m off Pearl and Hermes Atoll, Northwestern Hawaiian Islands. This species has been observed by mixed-gas divers and from submersibles at depths ranging from 45–187 m throughout the Hawaiian Archipelago, with shallower sightings in the Northwestern Hawaiian Islands and deeper in the Main Hawaiian Islands. It is similar to Prognathodes guezei (Maugé and Bauchot 1976) from the western Indian Ocean, and at least one other undescribed species of Prognathodes from Palau, differing from these species in the number of soft dorsal-fin rays, size of head, and body depth. There are also differences in the life color, and a substantial genetic difference from the Palauan species (d » .08 in mtDNA cytochrome oxidase I). PMID:27667937

  2. Taxonomic revision and stratigraphic provenance of 'Histiophorus rotundu' Woodward 1901 (Teleostei, Perciformes)

    USGS Publications Warehouse

    Monsch, K.A.; Fierstine, H.L.; Weems, R.E.

    2005-01-01

    Until recently, Histiophorus rotundus Woodward 1901, was known from a single, poorly preserved rostrum from the Tertiary phosphate beds near Charleston, South Carolina, an area from which many fossils have been described. The specimen is relatively featureless externally; its internal anatomy is unknown and the documentation of its geological provenance was poor. In an earlier revision the species was transferred to the fossil billfish genus Xiphiorhynchus Van Beneden, 1871. Here we confirm this designation, supported by new morphological studies of the holotype, recently found specimens of Xiphiorhynchus rotundus (Woodward, 1901), and the stratigraphic record of Xiphiorhynchus. The systematic paleontology we present is a contribution to the taxonomic revision of billfishes world-wide. Because the holotype is heavily phosphatized and the type locality was vaguely described, we discuss the geology of the phosphate mining districts of the Charleston region. Based on our studies, we can narrow the possible age of the holotype to late Oligocene or early Miocene. We suggest X. rotundus was extinct by the Burdigalian. 

  3. Plectranthias alcocki, a new anthiine fish species (Perciformes: Serranidae) from the Arabian Sea, off southwest India.

    PubMed

    Bineesh, K K; Akhilesh, K V; Gopalakrishnan, A; Jena, J K

    2014-04-04

    A new species of anthiine fish, Plectranthias alcocki n. sp. is described and illustrated based on two specimens, (63.7-72.5 mm SL), recently collected from deep-waters of the Arabian Sea, off Kollam, Kerala, India. The following combination of characters distinguishes it from all other congeners: Dorsal-fin rays X, 15; anal-fin rays III, 7; pectoral-fin rays 14, all unbranched; pelvic-fin rays I, 5; lateral-line complete, the pored lateral-line scales 28; scales above lateral line to origin of dorsal fin 1; scales dorsally on head extending to posterior nostrils; no scales on maxilla or chin; gill rakers 5 + 11 (2 + 7 developed); circumpeduncular scales 10; fourth dorsal spine longest, 2.8 (2.6) in head length (HL), longest dorsal-fin soft ray (second)  2.4 (2.7) in head length; body depth 34.4 (35)% SL; head length 46 (49.8)% SL; orbital length 8.6 in SL; margin of preopercle finely serrate, the serrae 33 (28), ventral edge without antrorse spines; dorsal fin continuous and notched; first anal-fin spine 4.9 (5.6) in HL, second anal-fin spine 2.2 (2.6) in HL; pelvic fins relatively short, 4.0-4.3 in SL; the dorsal fin with a black blotch at base of fourth to eighth spines, one at base of the last three spines, and two at base of soft portion of fin, the dark pigment extending onto adjacent body.

  4. Haliotrema banana sp. n. (Monogenea: Ancyrocephalidae) from Bodianus perditio (Perciformes: Labridae) off New Caledonia.

    PubMed

    Lim, L H S; Justine, Jean-Lou

    2007-09-01

    Sixteen labrid species, including four Bodianus spp., were examined in New Caledonia (South Pacific) and monogeneans were found only on Bodianus perditio (Quoy et Gaimard). This species, Haliotrema banana sp. n., is the second Haliotrema species to be described from the labrids, the first being Haliotrema bodiani Yamaguti, 1968 from Bodianus albotaeniatus (Valenciennes), previously designated as B. bilunulatus (Lacépède). The new species is similar to H. bodiani in soft reproductive parts but differs from it in the morphologies of the hard haptoral parts, mainly in the shape of the dorsal bar (bar-shaped vs V-shaped in H. bodiani) and ventral bar. It is similar to Haliotrema spirale Yamaguti, 1968 and Haliotrema minutospirale Yamaguti, 1968 in the shape of the anchors and bars but differs from them in the detailed structures of the copulatory organ and vaginal system.

  5. Pseudorhabdosynochus hirundineus n. sp. (Monogenea: Diplectanidae) from Variola louti (Perciformes: Serranidae) off New Caledonia.

    PubMed

    Justine, Jean-Lou

    2005-09-01

    Pseudorhabdosynochus hirundineus n. sp. is described from specimens collected from the gills of Variola louti off Nouméa, New Caledonia, South Pacific. No diplectanid was found on V. albimarginata from the same location. The male sclerotised quadriloculate organ of P. hirundineus is 42 microm in internal length, with a long posterior tube; and the sclerotised vagina, 36 microm in length, is composed of a long tube with anterior open trumpet and two posterior, heavily sclerotised chambers. The two squamodiscs, each with 11-15 rows of rodlets and no central closed row of rodlets, are dissimilar in shape, the ventral being round and the dorsal being oval. By the morphology of its sclerotised vagina, P. hirundineus appears close to P. epinepheli (Yamaguti, 1938) but it is differentiated from it by the shape of its squamodiscs. Pseudorhabdosynochus spp. previously recorded from epinephelines are parasites of species of Epinephelus and Mycteroperca; this is the first species from a species of Variola.

  6. Hamacreadium cribbi n. sp. (Digenea: Opecoelidae) from Lethrinus miniatus (Forster) (Perciformes: Lethrinidae) from New Caledonian waters.

    PubMed

    Bray, Rodney A; Justine, Jean-Lou

    2016-10-01

    A new species of Hamacreadium Linton, 1910, H. cribbi n. sp. is described from Lethrinus miniatus (Forster) from the waters off New Caledonia. It is compared with the other species of Hamacreadium reported from lethrinids and is characterised by the size of its eggs which tend to be larger [72-93 (84) vs 54-81 (56) µm long] than those of other species. Other characteristics, such as body size and shape and internal ratios, differentiate H. cribbi from other species; these differences are discussed in detail.

  7. Brain and sense organ anatomy and histology in hemoglobinless Antarctic icefishes (Perciformes: Notothenioidei: Channichthyidae).

    PubMed

    Eastman, Joseph T; Lannoo, Michael J

    2004-04-01

    The Channichthyidae, one of five Antarctic notothenioid families, includes 16 species and 11 genera. Most live at depths of 200-800 m and are a major component of fish biomass in many shelf areas. Channichthyids are unique among adult fishes in possessing pale white blood containing a few vestigal erythrocytes and no hemoglobin. Here we describe the brains of seven species and special sense organs of eight species of channichthyids. We emphasize Chionodraco hamatus and C. myersi, compare these species to other channichthyids, and relate our findings to what is known about brains and sense organs of red-blooded notothenioids living sympatrically on the Antarctic shelf. Brains of channichthyids generally resemble those of their bathydraconid sister group. Among channichthyids the telencephalon is slightly regressed, resulting in a stalked appearance, but the tectum, corpus cerebellum, and mechanoreceptive areas are well developed. Interspecific variation is present but slight. The most interesting features of channichthyid brains are not in the nervous tissue but in support structures: the vasculature and the subependymal expansions show considerable elaboration. Channichthyids have large accessory nasal sacs and olfactory lamellae are more numerous than in other notothenioids. The eyes are relatively large and laterally oriented with similar duplex (cone and rod) retinae in all eight species. Twin cones are the qualitatively dominant photoreceptor in histological sections and, unlike bathydraconids, there are no species with rod-dominated retinae. Eyes possess the most extensive system of hyaloid arteries known in teleosts. Unlike the radial pattern seen in red-blooded notothenioids and most other teleosts, channichthyid hyaloid arteries arise from four or five main branches and form a closely spaced anastomosing series of parallel channels. Cephalic lateral line canals are membranous and some exhibit extensions (canaliculi), but canals are more ossified than those of deeper-living bathydraconids. We conclude that, with respect to the anatomy and histology of the neural structures, the brain and sensory systems show little that is remarkable compared to other fishes, and exhibit little diversification within the family. Thus, the unusual habitat and a potentially deleterious mutation resulting in a hemoglobinless phenotype are reflected primarily in expansion of the vasculature in the brain and eye partially compensating for the absence of respiratory pigments. Neural morphology gives the impression that channichthyids are a homogeneous and little diversified group.

  8. Metazoan parasites of Geophagus brasiliensis (Perciformes: Cichlidae) in Patos Lagoon, extreme south of Brazil.

    PubMed

    Rassier, Gabriela Lopes; Pesenti, Tatiana Cheuiche; Pereira Júnior, Joaber; da Silva, Diego Silva; Wendt, Emília Welter; Monteiro, Cassandra de Moraes; Berne, Maria Elizabeth Aires

    2015-01-01

    This study has evaluated the parasitic fauna of 79 pearl cichlids (Geophagus brasiliensis) from the estuary of Patos Lagoon (31° 57' S and 52° 06' W), Rio Grande do Sul, Brazil, during the months of May and June in 2011 and 2012. All the hosts analyzed were infected with at least one species of parasite. A total of eleven metazoa were identified in 459 specimens collected. The trematode Austrodiplostomum compactum (34.2%) and ergasilids Ergasilus lizae (32.9%) and Gauchergasilus lizae (32.9%) were the most prevalent species. The trematodes Thometrema overstreeti and Posthodiplostomum sp. had significantly higher prevalence in fish longer than 20 cm. The sex of the host had no effect on parasite prevalence and abundance. Pearl cichlids are registered as a new host for the trematodes Lobatostoma sp., Homalometron pseudopallidum and Thometrema overstreeti, for the ergasilids Ergasilus lizae and Gauchergasilus euripedesi and for the argulid Argulus spinolosus. The crustacean E. lizae is recorded in Rio Grande do Sul for the first time.

  9. Rebuttal to Koeda et al. (2014) on the Red Sea fishes of the perciform genus Pempheris.

    PubMed

    Randall, John E; Victor, Benjamin C; Alpermann, Tilman J; Bogorodsky, Sergey V; Mal, Ahmad O; Satapoomin, Ukkrit; Bineesh, K K

    2014-11-25

    Koeda et al. (2014) published a review of fishes of the genus Pempheris of the Red Sea. They concluded that there are four species: P. adusta Bleeker, P. mangula Cuvier, P. nesogallica Cuvier, and a new species, P. tominagai. We show that the first three species they cite are not present in the Red Sea, as follows. 1) P. adusta is a western Pacific species (type locality Ambon), described only from the holotype, and without a dark border on the anal fin. Koeda et al. (2014) mistakenly apply that name to P. flavicycla which is a widespread Indian Ocean species characterized by a prominent broad black border along the anal fin. Koeda et al. (2014) also redescribe P. adusta, using Indian Ocean specimens of P. flavicycla, despite the coloration difference and a 2.5% difference in the mtDNA sequence (COI) between Indian Ocean and W. Pacific populations. 2) P. mangula is a species from the east coast of India (type locality Visakhapatnam), clearly distinct in both gill-raker counts and a 1.1% sequence divergence in COI from its Red Sea relative P. rhomboidea. Pempheris mangula is not found west of India, and Koeda et al. (2014) mistakenly use DNA from Oman and Madagascar to represent P. mangula, instead of genetic material available from the type locality. 3) Pempheris nesogallica (type locality Mauritius) is unknown from the Red Sea. Koeda et al. (2014) separate P. nesogallica from P. rhomboidea (their "P. mangula") by eye size; we fail to find any difference (and they use their purported eye-size difference to erroneously rename one of the two syntypes of P. nesogallica as "P. mangula"). 4) Their new species P. tominagai is referred to as the Indian Ocean sister species of "P. schwenkii of the Pacific"; however, the type locality of P. schwenkii is the Batu Islands off the SW coast of Sumatra in the Indian Ocean. They mistakenly include specimens of a distant South African species as paratypes of P. tominagai. We have determined that P. tominagai is a valid species endemic to the Red Sea and Gulf of Aden. They misidentify one lot of P. rhomboidea in the collection of the Hebrew University of Jerusalem as their record of P. nesogallica from the Red Sea. They misidentify the specimen in their photograph of Fig. 1B as P. adusta and use it as material for their redescription of the species, but it is now shown to be a paratype of Pempheris bexillon Mooi & Randall, 2014. Additionally, they regard P. malabarica Cuvier as a junior synonym of P. molucca Cuvier, but the name P. molucca is based on a fanciful painting and is unavailable as a nomen dubium. They treat Pempheris russellii Day as a junior synonym of P. mangula; however, it is distinct in having longer pectoral fins, a larger eye, and more gill rakers. Their key to the species of Pempheris of the Red Sea is incorrect. We present a new key and conclude that only three species of Pempheris are presently known from the Red Sea: P. flavicycla, P. rhomboidea, and P. tominagai.

  10. The complete mitochondrial genome of jade perch, Scortum barcoo (Perciformes: Terapontidae: Scortum).

    PubMed

    Liu, Chun; Li, Kaibin; Wang, Qing; Wang, Fang; Zeng, Weiwei; Mai, Xiaomei; Wu, Shuqin

    2016-09-01

    This is the first documentation of the complete mitochondrial genome sequence of the jade perch, Scortum barcoo. The 16,843 bp mitogenome containes 37 genes (13 protein-coding genes, 22 transfer RNA genes, and 2 ribosomal RNA genes) and two main non-coding regions (the control region and the origin of the light strand replication) as do other vertebrate mitogenomes. The overall base composition of S. barcoo is 27.39% for A, 24.74% for T, 31.14% for C and 16.73% for G, with a slight A + T bias of 52.13%. All protein-coding genes are initiated with ATG except for COX1, which begin with GTG instead. Meanwhile, the termination codons of 13 protein-coding genes are varied with TAA, TAG, TA, T or AGG. The measure of complete mitogenome sequence of S. barcoo will provide fundamental data for the phylogenetic and biogeographic studies of the Terapontidae.

  11. Tempo and rates of diversification in the South American cichlid genus Apistogramma (Teleostei: Perciformes: Cichlidae)

    PubMed Central

    García Dávila, Carmen R.; Römer, Uwe; Duponchelle, Fabrice; Cerqueira, Frédérique; Paradis, Emmanuel; Guinand, Bruno; Angulo Chávez, Carlos; Salas, Vanessa; Quérouil, Sophie; Sirvas, Susana; Renno, Jean-François

    2017-01-01

    Evaluating biodiversity and understanding the processes involved in diversification are noticeable conservation issues in fishes subject to large, sometimes illegal, ornamental trade purposes. Here, the diversity and evolutionary history of the Neotropical dwarf cichlid genus Apistogramma from several South American countries are investigated. Mitochondrial and nuclear markers are used to infer phylogenetic relationships between 31 genetically identified species. The monophyly of Apistogramma is suggested, and Apistogramma species are distributed into four clades, corresponding to three morphological lineages. Divergence times estimated with the Yule process and an uncorrelated lognormal clock dated the Apistogramma origin to the beginning of the Eocene (≈ 50 Myr) suggesting that diversification might be related to marine incursions. Our molecular dating also suggests that the Quaternary glacial cycles coincide with the phases leading to Apistogramma speciation. These past events did not influence diversification rates in the speciose genus Apistogramma, since diversification appeared low and constant through time. Further characterization of processes involved in recent Apistogramma diversity will be necessary. PMID:28873089

  12. Reproductive biology of common snook Centropomus undecimalis (Perciformes: Centropomidae) in two tropical habitats.

    PubMed

    Perera-García, Martha A; Mendoza-Carranza, Manuel; Contreras-Sánchez, Wilfrido M; Huerta-Ortíz, Maricela; Pérez-Sánchez, Eunice

    2011-06-01

    In Southeastern Mexico, Centropomus undecimalis is an important fish species of sport and commercial fisheries for coastal and riverine communities. Fisheries along rivers and coasts depend on migratory habits of this species, and these movements are probably related to reproduction. In spite of its economic importance, few studies have been conducted focusing on its reproductive biology, and this research aims to analyze these habits. Samples (fork length, somatic and gonads weight, and macroscopic maturity stages) were obtained from organisms collected by fishermen from the largest fishing cooperatives along the coastal and riverine areas of Tabasco, from July 2006 to March 2008. Fish size ranged from 34 to 112 cm fork length, with an average age of 6.42 years for males and 9.12 years for females. In riverine areas, fish sizes ranged from 30 to 85 cm and the average age was 5.5 years for males and 6.6 years for females. Significant differences were recorded between lengths of males and females from the two areas (Kruskal-Wallis, p < 0.05). The male:female ratio was 1:0.68 in the coast, and 1:0.16 in riverine areas. The length-weight relationship did not vary between both sexes among areas (ANCOVA, p > 0.05). A curve for eviscerated weight was calculated for both sexes, for coastal fishes SW = 0.0059 (FL)3.07, and the riverine ones SW = 0.0086 (FL) 2.98, with an isometric growth (b = 3). The period of maximum reproduction was from July to August, with temperatures of 28 to 30 degrees C. A significant correlation between the gonadosomatic index (GSI) and rainfall was recorded for samples of both males and females from coastal areas (r = 0.63, r = 0.70) whereas only one positive correlation was recorded for riverine females (r = 0.57). The size at first maturity (L50) was estimated at 60 cm and 80 cm (FL), corresponding to 5.5 and 8.5 years of age, for males and females, respectively. An important proportion of mature females of eight years and older, suggests that these ages contribute significantly to the reproductive biomass. The results indicate that due to changes in the exploitation period, we recommend to protect populations of the common snook.

  13. Protemblemaria perla, a new species of tube blenny (Perciformes: Chaenopsidae) from the tropical eastern Pacific.

    PubMed

    Hastings, P A

    2001-07-01

    Protemblemaria perla, from Isla del Rey, Islas Perlas, Panama, is distinctive in having a long unbranched cirrus on the posterior nostril, a U-shaped fleshy ridge bearing fleshy flaps and a pair of papillae on the nape, a relatively high number (18) of segmented dorsal-fin rays, and small rust-colored spots on the dorsal fin. Protemblemaria perla and P. punctata, from the southern Caribbean, share several morphological features and form a transisthmian species pair. Although the degree of color variation is unknown in P. perla, color varies greatly in P. bicirris and P. punctata, both of which have an orange morph.

  14. First record of Mugil capurrii (Mugilidae, Perciformes) in the Gulf of Guinea.

    PubMed

    Trape, S; Durand, J-D

    2011-03-01

    Leaping African mullet Mugil capurrii was caught along the Togolese coast in the Gulf of Guinea. This is the first record of this species which usually occurs from Morocco to Guinea Bissau and the southernmost point of its known distribution.

  15. Life history of the symbiotically luminous cardinalfish Siphamia tubifer (Perciformes: Apogonidae).

    PubMed

    Gould, A L; Dougan, K E; Koenigbauer, S T; Dunlap, P V

    2016-08-01

    Characteristics of the life history of the coral reef-dwelling cardinalfish Siphamia tubifer, from Okinawa, Japan, were defined. A paternal mouthbrooder, S. tubifer, is unusual in forming a bioluminescent symbiosis with Photobacterium mandapamensis. The examined S. tubifer (n = 1273) ranged in size from 9·5 to 43·5 mm standard length (LS ), and the minimum size at sexual maturity was 22 mm LS . The number of S. tubifer associated during the day among the spines of host urchins was 22·9 ± 16·1 (mean ± s.d.; Diadema setosum) and 3·6 ± 3·2 (Echinothrix calamaris). Diet consisted primarily of crustacean zooplankton. Batch fecundity (number of eggs; FB ) was related to LS by the equations: males (fertilized eggs) FB  = 27·5LS  - 189·46; females (eggs) FB  = 31·3LS  - 392·63. Individual mass (M; g) as a function of LS was described by the equation: M=9·74×10-5LS2·68. Growth, determined from otolith microstructure analysis, was described with the von Bertalanffy growth function with the following coefficients: L∞  = 40·8 mm LS , K = 0·026 day(-1) and t0  = 23·25 days. Planktonic larval duration was estimated to be 30 days. The age of the oldest examined individual was 240 days. The light organ of S. tubifer, which harbours the symbiotic population of P. mandapamensis, increased linearly in diameter as S. tubifer LS increased, and the bacterial population increased logarithmically with S. tubifer LS . These characteristics indicate that once settled, S. tubifer grows quickly, reproduces early and typically survives much less than 1 year in Okinawa. These characteristics are generally similar to other small reef fishes but they indicate that S. tubifer experiences higher mortality. © 2016 The Fisheries Society of the British Isles.

  16. Mitochondrial DNA sequence-based phylogenetic relationship of Trichiurus lepturus (Perciformes: Trichiuridae) from the Persian Gulf

    PubMed Central

    Tamadoni Jahromi, S.; Mohd Noor, S. A.; Pirian, K.; Dehghani, R.; Nazemi, M.; Khazaali, A.

    2016-01-01

    In this study, mitochondrial DNA analysis using 16S ribosomal DNA (rDNA) was performed to investigate the phylogeny relationship of Trichiurus lepturus in the Persian Gulf compared to the other investigated area. The amplification of 16S rDNA resulted in a product of 600 bp in all samples. The results showed that the isolated strain belongs to T. lepturus showing 42 divergence sites among the same reported partial sequences of 16S rRNA gene from the other area (West Atlantic and Indo-Pacific area). Phylogeny results showed that all 18 haplotypes of the species clustered into five clades with reasonably high bootstrap support of values (>64%). Overall, the tree topology for both phylogenetic and phenetic trees for 16S rDNA was similar. Both trees exposed two major clusters, one wholly containing the haplotypes of the T. lepturus species belonging to Indo-Pacific area with two major sister groups including Persian Gulf specimen and the other cleared the Western Atlantic and Japan individuals clustered in another distinct clade supporting the differentiation between the two areas. Phylogenic relationship observed between the Persian Gulf and the other Indo-Pacific Individuals suggested homogeneity between two mentioned areas. PMID:27822250

  17. Mitochondrial DNA sequence-based phylogenetic relationship of Trichiurus lepturus (Perciformes: Trichiuridae) from the Persian Gulf.

    PubMed

    Tamadoni Jahromi, S; Mohd Noor, S A; Pirian, K; Dehghani, R; Nazemi, M; Khazaali, A

    2016-01-01

    In this study, mitochondrial DNA analysis using 16S ribosomal DNA (rDNA) was performed to investigate the phylogeny relationship of Trichiurus lepturus in the Persian Gulf compared to the other investigated area. The amplification of 16S rDNA resulted in a product of 600 bp in all samples. The results showed that the isolated strain belongs to T. lepturus showing 42 divergence sites among the same reported partial sequences of 16S rRNA gene from the other area (West Atlantic and Indo-Pacific area). Phylogeny results showed that all 18 haplotypes of the species clustered into five clades with reasonably high bootstrap support of values (>64%). Overall, the tree topology for both phylogenetic and phenetic trees for 16S rDNA was similar. Both trees exposed two major clusters, one wholly containing the haplotypes of the T. lepturus species belonging to Indo-Pacific area with two major sister groups including Persian Gulf specimen and the other cleared the Western Atlantic and Japan individuals clustered in another distinct clade supporting the differentiation between the two areas. Phylogenic relationship observed between the Persian Gulf and the other Indo-Pacific Individuals suggested homogeneity between two mentioned areas.

  18. Embryonic and larval development of Eugerres mexicanus (Perciformes: Gerreidae) in Tenosique, Tabasco, Mexico.

    PubMed

    Hernández, Raúl E; Perera, Martha A; Castillo, Alfonso; Luna, Emiliano; de la Cruz, José A; Gómez, Luis M; Valdez Zenil, José

    2012-03-01

    Most studies on Eugerres mexicanus mainly consider biogeographic and systematic aspects and rarely address reproductive characteristics, which are useful for fishery population management plans. This study aimed at evaluating the ontogeny of E. mexicanus, based on 30 embryos and 30 larvae sampled by induced spawning of breeders, taken in February 2009 from the Usumacinta River in Tenosique, Tabasco, Mexico. All descriptions of the embryonic development were based on morphometric and meristic data and followed standard methods. Eggs, recovered at the gastrula stage, had an average diameter of 1.17mm (SD=0.08). The bud stage appeared during the first three hours of development, in which the posterior side was adhered to the vitellus; Kupffer's vesicle was visible. Yolk-sac larvae hatched 18 hours after fertilization, exhibiting a light brown color and an average total length of 2.94mm (SD=0.70); the preflexion stage was reached eight days after hatching, with a total average length of 4.67mm (SD=0.50) and a total notochord length of 4.45mm (SD=0.50). The flexion stage was reached on the 16th day, with an average total length of 6.66mm (SD=1.53), while postflexion was reached on the 24th day, with 10.33mm (SD=1.45). The pre-juvenile stage was reached on the 33rd day, with a total length of 14.30mm (SD=0.93), showing IX spines and 10 rays and III spines and eight rays in the dorsal and anal fins, respectively. The juvenile stage was reached by the 45th day, with an average length of 28.16mm (SD=1.93) and average weight of 4.75g (SD=1.49). Prejuveniles showed an initial pigmentation with dark colored dots in the superior and inferior jaw and dispersed on the head, while juveniles presented the same pigmentation pattern, decreasing towards the margin of the caudal peduncle. In conclusion, the embryonic developmental stages of E. mexicanus were typical for the Gerreidae group. However, their morphometric characters were slightly different since the diameter and size of the drop of oil were bigger than those reported for marine species. In addition, regarding pigmentation, the yolk-sac larvae of E. mexicanus were olive and yellow on the margin of the notochord, which differs from those reported for other species. This is the first recorded report on the reproductive biology and early life development of this species.

  19. The gallbladder of Uranoscopus scaber L. (teleost perciform fish) is lined by specialized cholecystocytes.

    PubMed

    Gilloteaux, J; Ott, Donald W; Oldham-Ott, Carla K

    2011-11-01

    The gallbladder of Uranoscopus exhibits a mucosal surface layer of simple columnar epithelium composed of specialized cholecystocytes. The apices show storage and mucous secretions, typical microvilli, and very apical projections extending deep into the luminal contents. Many organelles and heterogeneous vesicles of diverse size fill the cytoplasm, including neutral mucins, mitochondria, peroxisomes, lysosomal bodies, and lipid-rich deposits with cholesterol inclusions. The fibromuscular layer shows little blood supply and contains scattered lymph-like walls with minute cholesterol inclusions. The remaining muscular, subserosal, and serosal or adventitial layers of this species do not show any histologic differences to those of other vertebrates. It was unexpected to find cholesterol inclusions in the fatty deposits of the cholecystocytes, similar to those noted in human cholesterolosis and in some forms of hypercholesterolemia, in this teleostean. In addition, aggregations of mitochondria and anomalous mitochondrial morphologies were found that resemble oncocytoma-like changes.

  20. Complete mitochondrial genome sequence of the spinyhead croaker Collichthys lucidus (Perciformes, Sciaenidae) with phylogenetic considerations.

    PubMed

    Cheng, Jiao; Ma, Guo-Qiang; Miao, Zhen-Qing; Shui, Bo-Nian; Gao, Tian-Xiang

    2012-04-01

    The complete mitochondrial genome of the spinyhead croaker Collichthys lucidus was determined using long-PCR and primer walking methods. It is a circular molecule of 16,451 bp in length with a standard set of 22 tRNAs, 2 rRNAs, 13 protein-coding genes as well as a non-coding control region in the same order as those of the other bony fishes. C. lucidus mitogenome exhibited a clear strand-specific bias in nucleotide composition, as evidence by a GC- skew of the H-strand of -0.319. The strand-specific bias was also reflected in the codon usage of genes oriented in opposite directions. All tRNA genes except for tRNA( Ser(AGY)) harbored the typical cloverleaf secondary structures and possessed anticodons that matched the vertebrate mitochondrial genetic code. Contrary to the typical structure of control region consistig of TAS, central, and CSB domains, there were no central conserved blocks available in C. lucidus mitogenome. Despite extensive studies based on both morphology and molecules, phylogenetic position of C. lucidus with Sciaenidae is still controversial. Our phylogenetic results provided more evidence to support previous morphological studies and consistently placed C. lucidus as a sister taxon to Collichthys niveatus, with both of these taxa forming the monophyletic Collichthys.

  1. The complete mitochondrial genome of the amoy croaker Argyrosomus amoyensis (Perciformes, Sciaenidae).

    PubMed

    Yang, Huirong; Zhao, Huihong; Sun, Jijia; Yang, Zituo; Xiao, Shibin; Li, Guifeng; Liu, Li

    2016-01-01

    We present the complete mitochondrial genome of Argyrosomus amoyensis in this study. The mitochondrial genome is 16,490 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. All of them are encoded on the heavy strand except ND6 and 8 tRNA genes on the light strand. The nucleotide compositions of the light strand are 31.02% of G, 26.96% of T, 25.31% of A and 16.71% of C. All the protein-coding genes begin with ATG initiation codon, and 5 types of inferred termination codons are AGA (COXI), T (COXII, ND3, ND4, Cytb), TA (ND2, COXIII), TAA (ATP8, ATP6, ND4L, ND5, ND6) and TAG (ND1). There are 11 intergenic spacers, 7 gene overlaps and no tandem repeat sequence. Gene arrangement and distribution are consistent with the typical vertebrates.

  2. Development of spermatogonial cell transplantation in Nibe croaker, Nibea mitsukurii (Perciformes, Sciaenidae).

    PubMed

    Takeuchi, Yutaka; Higuchi, Kentaro; Yatabe, Takashi; Miwa, Misako; Yoshizaki, Goro

    2009-12-01

    In a recently established system for intraperitoneal spermatogonial cell transplantation in salmonids, donor type A spermatogonia (type A SG) were microinjected into the peritoneal cavity of newly hatched larvae. Compared with salmonids, the larvae of marine teleosts are small and vulnerable to physiological and physical stresses, making it difficult to use them for cell manipulation. Herein, we developed type A SG cell transplantation in Nibe croaker (Nibea mitsukurii) by optimizing 1) the developmental stage of the donor testes used to prepare type A SG-enriched cell suspensions and 2) the timing and location of intraperitoneal cell transplantations to recipient larvae. Donor cells labeled with PKH26 fluorescent dye were transplanted into the peritoneal cavity of 3-, 4-, 5-, and 6-mm larvae using glass micropipettes. Consequently, 20.6% of the 4-mm larvae recipients survived for 3 wk, and 36.3% of the survivors had donor-derived cells in their gonads. The incorporated donor cells were identified as germ cells by germ cell-specific nuclear morphology and expression of a germ cell marker. In contrast, no donor type A SG were incorporated into the gonads of 6-mm recipient larvae. These data indicate that there is a distinct narrow window in the developmental stages of recipient larvae when exogenous type A SG can be incorporated into the gonads. The establishment of this system in pelagic egg-spawning marine teleosts would allow the creation of a new broodstock system in which a target species with a large body size and long generation time could be produced from related species with a small body size and short generation time.

  3. Mitochondrial genome sequence of the Japanese white croaker Pennahia argentata (Perciformes: Sciaenidae).

    PubMed

    Li, Ang; Sun, Dianrong; Li, Yuan; Gao, Tianxiang

    2014-04-01

    Through primer walking method, the mitochondrial genome sequence of white croaker (Pennahia argentata) from Japanese coastal water was obtained in this study. The mitogenome length of Japanese white croaker was 16,486 bp. The genome contains 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes, a control region (CR) and a dominating non-coding region. The termination-associated sequence, the central conserved domain, and the conserved sequence blocks were identified in the control region. The origin of L-strand replication was located in a cluster of five tRNA genes between tRNA-Asn and tRNA-Cys.

  4. The complete mitochondrial genome of the blackspotted croaker Protonibea diacanthus (Perciformes, Sciaenidae).

    PubMed

    Liu, Li; Yang, Huirong; Yang, Zituo; Zhao, Huihong; Sun, Jijia; Xiao, Shibin; Yang, Xiankuan; Li, Guifeng

    2016-05-01

    We present the complete mitochondrial genome of Protonibea diacanthus in this study. The mitochondrial genome is 16,535 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and a control region. All of them are encoded on the heavy strand except ND6 and 8 tRNA genes on the light strand. The nucleotide compositions of the light strand are 31.09% of G, 27.54% of T, 25.34% of A and 16.03% of C. All the protein-coding genes start with ATG initiation codon except ATP6 with GTG, and 5 types of inferred termination codons are AGA (COXI and COXII), T (ND3, ND4, Cytb), TA (ND2, COXIII and ATP6), TAA (ND1, ATP8 and ND4L) and TAG (DN5 and ND6). There are 14 intergenic spacers and 7 gene overlaps. The tandem repeat sequence is observed in 12S-rRNA, 16S-rRNA, tRNA(Trp), tRNA(Ala), COXIII, ND4L and ND4 genes. Gene arrangement and distribution are consistent with the typical vertebrates.

  5. The oldest fossil cichlids (Teleostei: Perciformes): indication of a 45 million-year-old species flock.

    PubMed Central

    Murray, A. M.

    2001-01-01

    Five closely related species of fossil cichlids collected from an Eocene site in Tanzania, East Africa, represent the oldest known cichlids. The specimens are whole-body, articulated fishes that are extremely well preserved and, therefore, have the potential to add to our knowledge of the history of this family. Modern cichlids are particularly well known for the numerous species flocks of the East African Great Lakes. A great deal of research is ongoing regarding all aspects of the fishes in these flocks, including their evolutionary history The new collection of fossils reported here is interpreted as representing a species flock that arose in a small crater lake. These fossils indicate that cichlids' ability to form species flocks evolved early in the history of this family. PMID:11321055

  6. DNA Barcoding Reveals Cryptic Diversity within Commercially Exploited Indo-Malay Carangidae (Teleosteii: Perciformes)

    PubMed Central

    Mat Jaafar, Tun Nurul Aimi; Taylor, Martin I.; Mohd Nor, Siti Azizah; de Bruyn, Mark; Carvalho, Gary R.

    2012-01-01

    Background DNA barcodes, typically focusing on the cytochrome oxidase I gene (COI) in many animals, have been used widely as a species-identification tool. The ability of DNA barcoding to distinguish species from a range of taxa and to reveal cryptic species has been well documented. Despite the wealth of DNA barcode data for fish from many temperate regions, there are relatively few available from the Southeast Asian region. Here, we target the marine fish Family Carangidae, one of the most commercially-important families from the Indo-Malay Archipelago (IMA), to produce an initial reference DNA barcode library. Methodology/Principal Findings Here, a 652 bp region of COI was sequenced for 723 individuals from 36 putative species of Family Carangidae distributed within IMA waters. Within the newly-generated dataset, three described species exhibited conspecific divergences up to ten times greater (4.32–4.82%) than mean estimates (0.24–0.39%), indicating a discrepancy with assigned morphological taxonomic identification, and the existence of cryptic species. Variability of the mitochondrial DNA COI region was compared within and among species to evaluate the COI region's suitability for species identification. The trend in range of mean K2P distances observed was generally in accordance with expectations based on taxonomic hierarchy: 0% to 4.82% between individuals within species, 0% to 16.4% between species within genera, and 8.64% to 25.39% between genera within families. The average Kimura 2-parameter (K2P) distance between individuals, between species within genera, and between genera within family were 0.37%, 10.53% and 16.56%, respectively. All described species formed monophyletic clusters in the Neighbour-joining phylogenetic tree, although three species representing complexes of six potential cryptic species were detected in Indo-Malay Carangidae; Atule mate, Selar crumenophthalmus and Seriolina nigrofasciata. Conclusion/Significance This study confirms that COI is an effective tool for species identification of Carangidae from the IMA. There were moderate levels of cryptic diversity among putative species within the central IMA. However, to explain the hypothesis of species richness in the IMA, it is necessary to sample the whole family across their broad geographic range. Such insights are helpful not only to document mechanisms driving diversification and recruitment in Carangidae, but also to provide a scientific framework for management strategies and conservation of commercially-important fisheries resources. PMID:23209586

  7. Review of the South American-Antarctic triplefin fish genus Helcogrammoides (Perciformes: Tripterygiidae).

    PubMed

    Williams, J T; Springer, V G

    2001-07-01

    The genus Helcogrammoides comprises three species of small, cryptic shorefishes, H. antarcticus, H. chilensis, and H. cunninghami. All three species inhabit shallow water along exposed rocky coasts. Specimens reported herein extend the known distributions of H. chilensis and H. cunninghami to the vicinity of Lima, Peru, approximately 1600 km north of their previously reported northernmost records in Chile. Helcogrammoides chilensis and H. cunninghami occur sympatrically over most of their ranges in Peru and Chile and are frequently taken together in the same field collections. Lectotypes are designated for H. chilensis and H. cunninghami. Diagnoses and an identification key are provided for the species.

  8. A new record of ponyfish Deveximentum megalolepis (Perciformes: Leiognathidae) in Beibu Gulf of China

    NASA Astrophysics Data System (ADS)

    Ju, Yuman; Song, Na; Chen, Guobao; Sun, Dianrong; Han, Zhiqiang; Gao, Tianxiang

    2017-06-01

    A new record ponyfish, Deveximentum megalolepis Mochizuki and Hayashi, 1989, was documented based on its morphological characteristics and DNA barcode. Fifty specimens were collected from Beibu Gulf of China and identified as D. megalolepis by morphological characterization. The coloration, meristic traits, and morphometric measurements were consistent with previously published records. In general, it is a silver-white, laterally compressed and deep bodied ponyfish with 6-9 rows of scales on cheek; scale rows above lateral line 6-8; scale rows below lateral line 14-17. Mitochondrial cytochrome oxidase I subunit (COI) gene fragment was sequenced for phylogenetic analysis. There is no sequence variation of COI gene between the specimens collected in this study. The genetic distances between D. megalolepis and other congeneric species range from 3.6% to 14.0%, which were greater than the threshold for fish species delimitation. The COI sequence analysis also supported the validity of D. megalolepis at genetic level. However, the genetic distance between Chinese and Philippine individuals was about 1.2% and they formed two lineages in gene tree, which may be caused by the geographical distance.

  9. [Growth and mortality parameters of Orthopristis ruber (Perciformes: Haemulidae) from Los Frailes Archipelaeo. Venezuela].

    PubMed

    Atenas, Guerrieri; Eslava, Nora; González, Leo Walter; Guevara, Francisco

    2015-03-01

    Orthopristis ruber is a species in high demand in Eastern Venezuela, but production has been decreasing in recent years. For this reason, our objective was to estimate the growth and mortality parameters of this resource. Monthly samples were collected from June 2011 to May 2012, obtaining 2980 specimens in El Tirano and Puerto Abajo. Data on total length (cm), total weight (g), and sex were recorded for each specimen. No sexual dimorphism was shown with respect to length (t(S)=1.113, p>0.05), so one length-weight ratio was established for both sexes (Wt=0.0612*Lt(2.54)); and they both exhibited minor allometric growth. Growth was estimated by analysis of length frequency distributions using FiSAT software. The estimated growth parameters (L.=39.03cm, W.=679.60g, k=0.48/year and t(O) = -0.32 year) showed moderately rapid growth. Length frequency data were adjusted to the von Bertalaniffy model, and indicated an exponential tendency of accelerated growth during the first years of life, followed by slow growth until the fish reached its maximum length. The coefficient of variation of the growth index (theta) demonstrated no differences in growth pattern. The natural mortality rate (M=0.97/year), from fishing (F=1.57/year), and total mortality (Z=2.54/year), were high, as well as the exploitation rate (E=0.62/year). We concluded that O. ruber has been fully exploited by artisanal fishers, and suggest a continuous study on population dynamics, to recommend optimum management techniques for the fishery.

  10. First record of red filament threadfin bream, Nemipterus marginatus (Valenciennes, 1830) (Perciformes, Nemipteridae), from Chinese waters

    NASA Astrophysics Data System (ADS)

    Ning, Ping; Wu, Renxie; Liu, Jing

    2011-11-01

    We collected five specimens of threadfin bream from Beihai, Guangxi, China in March 2010. These were subsequently identified as red filament threadfin bream Nemipterus marginatus (Valenciennes, 1830), being the first record of this species from Chinese waters. N. marginatus is distinguished by the following characteristics: lower border of eye lies above a line from tip of snout to upper base of pectoral fin; mouth oblique, maxillary extending to lower anterior border of pupil; teeth in jaws in several rows, pointed; upper jaw with 3 to 5 pairs of small recurved canines; suborbital with straight lower edge and rounded posterior edge; pectoral fins extending to between level of anus and origin of anal fin; pelvic fins reaching to the first or second anal rays; caudal fin forked, upper lobe tails into a short reddish filament; dorsal fin bluish with a yellow margin distally and a broad yellow median band which subdivides posteriorly into 3 small bands.

  11. Meals for two: foraging activity of the butterflyfish Chaetodon striatus (Perciformes) in southeast Brazil.

    PubMed

    Bonaldo, R M; Krajewski, J P; Sazima, I

    2005-05-01

    The banded butterflyfish (Chaetodon striatus) from the tropical and subtropical western Atlantic is a territorial, diurnal forager on benthic invertebrates. It is usually seen moving singly or in pairs, a few meters above the sea floor. We studied the foraging activity of C. striatus on rocky reefs in southeastern Brazil. This fish spent about 11 h and 30 min per day on feeding activities, and preferred colonies of non-scleratinian anthozoans over sandy and rocky substrata while foraging. The lowest feeding rates were recorded in the early morning and late afternoon, but we found no further differences between feeding rates throughout the day. We also found no differences between the feeding rates of paired and single individuals.

  12. Diet-Morphology Correlations in the Radiation of South American Geophagine Cichlids (Perciformes: Cichlidae: Cichlinae)

    PubMed Central

    López-Fernández, Hernán; Winemiller, Kirk O.; Montaña, Carmen; Honeycutt, Rodney L.

    2012-01-01

    Genera within the South American cichlid tribe Geophagini display specialized feeding and reproductive strategies, with some taxa specialized for both substrate-sifting and mouth brooding. Several lineages within the clade also possess an epibranchial lobe (EBL), a unique pharyngeal structure that has been proposed to have a function in feeding and/or mouth brooding. A recently published genus-level phylogeny of Neotropical cichlids was used as the evolutionary framework for investigating the evolution of morphological features presumably correlated with diet and mouth brooding in the tribe Geophagini. We tested for possible associations between the geophagine epibranchial lobe and benthic feeding and mouth brooding. We also addressed whether the EBL may be associated with unique patterns of diversification in certain geophagine clades. Tests of binary character correlations revealed the EBL was significantly associated with mouth brooding. We also tested for a relationship between diet and morphology. We analyzed stomach contents and morphometric variation among 21 species, with data for two additional species obtained from the literature. Principal Components Analysis revealed axes of morphological variation significantly correlated with piscivory and benthivory, and both morphology and diet were significantly associated with phylogeny. These results suggest that the EBL could be an adaptation for either feeding or mouth brooding. The EBL, however, was not associated with species richness or accelerated rates of phyletic diversification. PMID:22485154

  13. Greenwoodochromini Takahashi from Lake Tanganyika is a junior synonym of Limnochromini poll (Perciformes: Cichlidae).

    PubMed

    Takahashi, T

    2014-04-01

    The infraorbitals (IOs) of four species endemic to Lake Tanganyika were examined and on the basis of this information and previous morphological and molecular studies, the tribe Greenwoodochromini is synonymized with the tribe Limnochromini and a new combination for Limnochromis abeelei and Limnochromis staneri is proposed: Greenwoodochromis abeelei and Greenwoodochromis staneri. The revised tribe Limnochromini, comprising 10 species belonging to seven genera, is characterized by IOs representing types G and I. The revised genus Greenwoodochromis, which consists of four species, is characterized by IOs representing type I. © 2014 The Fisheries Society of the British Isles.

  14. [Reproductive biology of Anisotremus interruptus (Perciformes: Haemulidae) in the Mexican Central Pacific].

    PubMed

    Ruiz-Ramírez, Salvador; Lucano-Ramírez, Gabriela; González-Sansón, Gaspar; Rojo-Vázquez, J A; Arellano-Martínez, Marcial

    2012-06-01

    Anisotremus interruptus is a widely distributed and commercially important species in the Eastern Pacific. A multi-year research on the reproduction of this species was carried out in coastal waters of Jalisco, Mexico. For this purpose, monthly samples were gathered with gillnets of different mesh sizes from 1998-2008. A total of 1090 individuals were collected with a total length range between 15.6-61.0cm. Significant differences from expected 1:1 ratio were found for sex ratios in the total sample and for each size class, but not when comparisons among months and years were done. Temporal variations of the gonadosomatic index and proportions of maturation stages suggest that the main reproductive period is from February to May, although some less intensive spawning events were observed in other months. Seven stages of oocytes development were identified. Oocytes in different stages of development were identified in ovaries classified as mature, suggesting an asynchronic type of gonad development. Mean maturation length (L50) was 31.0 for females and 29.7cm for males. The internal organization of testes is of lobular type.

  15. Phylogenetic relationships and a new classification of the family Cyclopteridae (Perciformes: Cottoidei).

    PubMed

    Oku, Kanami; Imamura, Hisashi; Yabe, Mamoru

    2017-01-16

     Phylogenetic relationships of the family Cyclopteridae were reconstructed based on osteological and external characters.  The monophyly of the family was strongly supported by 47 commonly recognized synapomorphies, including six autapomorphies (plus one additional autapomorphy, presence of a dorsal process on the pelvis, recognized after the phylogenetic analysis) among the suborder Cottoidei.  As a result of the cladistic analysis, a single most parsimonious phylogeny was obtained, based on characters in 32 transformation series.  A new classification of Cyclopteridae based on reconstructed relationships, including three subfamilies [Liparopsinae, Cyclopterinae and Eumicrotreminae (newly established)] and four genera (Aptocyclus, Cyclopsis, Cyclopterus and Eumicrotremus), is proposed.

  16. Paranthias furcifer (Perciformes: Serranidae), a new alien fish in the Mediterranean Sea.

    PubMed

    Dulčić, J; Dragičević, B

    2013-01-01

    A specimen of a creole fish Paranthias furcifer (285 mm total length) was captured in Marina Bay (Croatian coast) in the eastern Adriatic Sea. This is the first Mediterranean record of this species. The possible modes of introduction of species are discussed. © 2012 The Authors. Journal of Fish Biology © 2012 The Fisheries Society of the British Isles.

  17. Luzonichthysseaver, a new species of Anthiinae (Perciformes, Serranidae) from Pohnpei, Micronesia.

    PubMed

    Copus, Joshua M; Ka'apu-Lyons, Cassie A; Pyle, Richard L

    2015-01-01

    Luzonichthysseaver, n. sp., is described from two specimens, 42-46 mm standard length (SL) collected from Pohnpei, Micronesia. Collections were made by divers on mixed-gas closed-circuit rebreathers using hand nets at depths of 90-100 m. Luzonichthysseaver is distinct from all other species of the genus in the characters of lateral line scales, gill rakers, pelvic fin length, caudal concavity and coloration. Of the six species of Luzonichthys, it appears to be morphologically most similar to L.earlei and L.whitleyi.

  18. Fecundity, growth, and survival of the angelfish Pterophyllum scalare (Perciformes: Cichlidae) under laboratory conditions.

    PubMed

    Ortega-Salas, Armando A; Cortés G, Isabel; Reyes-Bustamante, Hugo

    2009-09-01

    The freshwater angelfishes (Pterophyllum) are South American cichlids that have become very popular among aquarists, yet scarce information on their culture and aquarium husbandry exists. We studied Pterophyllum scalare to analyze dietary effects on fecundity, growth, and survival of eggs and larvae during 135 days. Three diets were used: A) decapsulated cysts of Artemia, B) commercial dry fish food, and C) a mix diet of the rotifer Brachionus plicatilis and the cladoceran Daphnia magna. The initial larval density was 100 organisms in each 40 L aquarium. With diet A, larvae reached a maximum weight of 3.80 g, a total length of 6.3 cm, and a height of 5.8 cm; with diet B: 2.80 g, 4.81 cm, and 4.79 cm, and with diet C: 3.00 g, 5.15 cm, and 5.10 cm, respectively. Significant differences were observed between diet A, and diet B and C, but no significantly differences were observed between diets B and C. Fecundity varied from 234 to 1,082 eggs in 20 and 50 g females, respectively. Egg survival ranged from 87.4% up to 100%, and larvae survival (80 larvae/40 L aquarium) from 50% to 66.3% using diet B and A, respectively. Live food was better for growing fish than the commercial balanced food diet. Fecundity and survival are important factors in planning a good production of angelfish.

  19. Altrichthys alelia, a new brooding damselfish (Teleostei, Perciformes, Pomacentridae) from Busuanga Island, Philippines

    PubMed Central

    Bernardi, Giacomo; Longo, Gary C.; Quiros, T.E. Angela L.

    2017-01-01

    Abstract A new species of damselfish, Altrichthys alelia sp. n. is described from specimens collected in shallow water (1–8m depth) off Busuanga Island, Palawan Province, Philippines. It differs from the other two species in the genus, A. curatus and A. azurelineatus, in various features including having golden upper body lacking dark edges of dorsal and caudal fins, higher modal number of tubed lateral line scales, as well as differences in two mitochondrial markers, one nuclear marker, and RAD markers. PMID:28769678

  20. Altrichthys alelia, a new brooding damselfish (Teleostei, Perciformes, Pomacentridae) from Busuanga Island, Philippines.

    PubMed

    Bernardi, Giacomo; Longo, Gary C; Quiros, T E Angela L

    2017-01-01

    A new species of damselfish, Altrichthys aleliasp. n. is described from specimens collected in shallow water (1-8m depth) off Busuanga Island, Palawan Province, Philippines. It differs from the other two species in the genus, A. curatus and A. azurelineatus, in various features including having golden upper body lacking dark edges of dorsal and caudal fins, higher modal number of tubed lateral line scales, as well as differences in two mitochondrial markers, one nuclear marker, and RAD markers.

  1. A new species of Percina (Perciformes: Percidae) from the Apalachicola River drainage, southeastern United States

    USGS Publications Warehouse

    Freeman, Mary C.; Freeman, B.J.; Burkhead, N.M.; Straight, C.A.

    2008-01-01

    Percina crypta, the Halloween Darter, is described as a new species endemic to the Chattahoochee and Flint River systems in Georgia and Alabama. Percina crypta differs from sympatric Percina nigrofasciata in having narrowly separated dorsal saddles (inter-saddle spaces typically less than or equal to saddle width, compared to frequently wider than saddle width in P. nigrofasciata), in usually possessing a single modified scale between the pelvic bases (compared to two or more in P. nigrofasciata), and in having dark wide bands on pectoral-fin rays (versus pectoral fin clear, or with irregular dark marks or weak tessellations on fin rays in P. nigrofasciata). Phylogenetic relationships of P. crypta to other species of Percina are obscure. Percina crypta occurs in shoal and riffle habitats in the Chattahoochee and Flint River mainstems and in a few tributary systems, with the known extant range comprising four disjunct areas separated by mainstem impoundments and altered river reaches.

  2. Tempo and rates of diversification in the South American cichlid genus Apistogramma (Teleostei: Perciformes: Cichlidae).

    PubMed

    Tougard, Christelle; García Dávila, Carmen R; Römer, Uwe; Duponchelle, Fabrice; Cerqueira, Frédérique; Paradis, Emmanuel; Guinand, Bruno; Angulo Chávez, Carlos; Salas, Vanessa; Quérouil, Sophie; Sirvas, Susana; Renno, Jean-François

    2017-01-01

    Evaluating biodiversity and understanding the processes involved in diversification are noticeable conservation issues in fishes subject to large, sometimes illegal, ornamental trade purposes. Here, the diversity and evolutionary history of the Neotropical dwarf cichlid genus Apistogramma from several South American countries are investigated. Mitochondrial and nuclear markers are used to infer phylogenetic relationships between 31 genetically identified species. The monophyly of Apistogramma is suggested, and Apistogramma species are distributed into four clades, corresponding to three morphological lineages. Divergence times estimated with the Yule process and an uncorrelated lognormal clock dated the Apistogramma origin to the beginning of the Eocene (≈ 50 Myr) suggesting that diversification might be related to marine incursions. Our molecular dating also suggests that the Quaternary glacial cycles coincide with the phases leading to Apistogramma speciation. These past events did not influence diversification rates in the speciose genus Apistogramma, since diversification appeared low and constant through time. Further characterization of processes involved in recent Apistogramma diversity will be necessary.

  3. Systematics, biogeography, and evolution of the Neotropical peacock basses Cichla (Perciformes: Cichlidae).

    PubMed

    Willis, Stuart C; Nunes, Mario S; Montaña, Carmen G; Farias, Izeni P; Lovejoy, Nathan R

    2007-07-01

    To investigate forces influencing diversification in Neotropical fishes, the phylogenetic relationships among species and populations of the cichlid genus Cichla were examined. Mitochondrial DNA was sequenced for 454 individuals of the 5 nominal Cichla species and several putative undescribed species. Phylogenetic analyses support the distinction of two major clades of Cichla. Clade A includes C. temensis and two undescribed species from the lower Amazonas and Xingu Rivers. Clade B includes C. orinocensis, C. monoculus, C. ocellaris. C, intermedia, and an undescribed species from the upper Madeira River. Species boundaries were relatively well-circumscribed for clade B, while incomplete lineage sorting was inferred for clade A. Three probable instances of introgression were observed, including a regional population of C. orinocensis from the Negro River that shows a history of introgression. Biogeographic patterns from Cichla are partially congruent with those seen in several other Neotropical fish clades, and the diversification of Cichla species is inferred to result from both vicariance and sympatric divergence.

  4. Systemic nocardiosis in a Mediterranean population of cultured meagre, Argyrosomus regius Asso (Perciformes: Sciaenidae).

    PubMed

    Elkesh, A; Kantham, K P L; Shinn, A P; Crumlish, M; Richards, R H

    2013-02-01

    Marine cultured meagre, Argyrosomus regius Asso, in central and western Greece were affected by an outbreak of systemic granulomatous disease subsequently demonstrated to be nocardiosis. The fish were originally imported as juveniles from hatcheries in France and Italy and on-grown in Greece, the latter also providing broodstock for a small number of local Greek hatcheries for the production of second-generation juveniles. The disease in cage reared fish had been present throughout the year, particularly in the 1+ and 2+ year old fish with a low to variable morbidity and 1-4% total mortality. Multiple lesions were visible externally on the skin of affected fish, with severe ulcerations and necrosis. Internally, multifocal yellowish-white nodules, 0.1-0.5 cm in diameter, were visible on the surface of several internal organs. Histopathology revealed systemic granulomatous inflammation. Fite-Faraco staining clearly demonstrated the presence of Nocardia-like organisms which were Gram-positive, long, rod to beaded filamentous bacteria. Nocardia genus-specific 16s RNA primers NG1 and NG2 were used to generate a 600 bp fragment recovered from affected tissue, confirming the diagnosis of Nocardia spp. To our knowledge, this is the first report of nocardiosis in meagre. © 2012 Blackwell Publishing Ltd.

  5. Molecular phylogeny and possible scenario of ponyfish (Perciformes:Leiognathidae) evolution.

    PubMed

    Ikejima, Kou; Ishiguro, Naoya B; Wada, Minoru; Kita-Tsukamoto, Kumiko; Nishida, Mutsumi

    2004-06-01

    The family Leiognathidae, commonly known as ponyfish or slip mouth, comprises three genera, each being characterized mainly by mouth morphology. To date, however, neither the phylogenetic relationships within the family nor monophyly of the genera has been tested. The phylogenetic relationships among 14 species of Leiognathidae, inferred from two protein coding mitochondrial genes (ND4 and 5), indicated monophyly of the studied species form genera Gazza and Secutor, and paraphyly of the genus Leiognathus, with L. equulus occupying a basal branch of the family. The relationships allowed phylogenetic analyses of mouthpart structures and light organ systems. The results suggested that the morphology of the upwardly and forwardly protractile mouth types (latter with canine-like teeth) are phylogenetically informative, and the downwardly protractile mouth type being ancestral in the family. The results also suggested that internal sexual dimorphism of the light organ system was present in the common ancestor of a sister clade to L. equulus, whereas external sexual dimorphism seems to have evolved subsequently in two monophyletic subgroups.

  6. Description of a new species of the cardinalfish genus Pseudamiops (Perciformes, Apogonidae) from the Red Sea.

    PubMed

    Gon, Ofer; Bogorodsky, Sergey V; Mal, Ahnad O

    2013-01-01

    A new species of pseudamine cardinalfish, Pseudamiops springeri, is described from five specimens collected in the Red Sea. It may be distinguished from the four known species of Pseudamiops in having 13 pectoralfin rays, pseudobranch with 2-4 filaments, no canine teeth on the vomer, anterior nostril with a conspicuous skin flap and genital opening flanked by two slender papillae. Pseudamiops springeri is closest to its Western Indian Ocean congener P. pellucidus Smith.

  7. Testis structure, spermatogenesis, spermatocytogenesis, and sperm structure in cardinal fish (Apogonidae, Perciformes).

    PubMed

    Fishelson, Lev; Delarea, Yacob; Gon, Ofer

    2006-01-01

    The testes in all 16 of the studied cardinal fish species are shown to be bilobed, with spermatogonia dispersed throughout the gametogenic epithelium of the seminiferous tubules. Each testicular lobe is covered luminally by an epithelium consisting of primary germ cells and Sertoli cells. At maturation the seminiferous tubules reach around 0.6-2.3 mm in length. They number from 60 in the smallest species to over 300 in the largest one, increasing both in dimension and number with increase in length of the male, and are species-specific. The highest number of spermatogonia is found at the apical ends of the tubules. During maturation extensions of Sertoli cells surround single or small groups of B-spermatogonia, forming the spermatocysts, the final dimensions of which reflect the final number of contained spermatozoids. Back-calculations of serial sections reveal that within the spermatocysts the spermatogonia undergo eight generations of mitotic divisions before the first and second meiotic divisions and formation of spermatids. The largest mature spermatocysts in large species attain around 180 microm in diameter, a volume of 25 mm(3), and contain around 8,200 spermatids. The total volume of sperm in the mature spermatocysts leaves enough space for the discarded cytoplasm and developing flagella. The bursting cysts liberate the ripe sperm and maturing spermatids, into the tubule lumen and spermduct, with the spermatids often still connected by cytoplasm bridges. The sperm, with one or two flagella, features round or oval heads and a cytoplasmic collar bearing a few mitochondria. The percentage of biflagellate or monoflagellate sperm differs in proportion in males of different lengths and in different species. Differences in spermatogenesis of small and larger species of cardinal fish are discussed.

  8. DNA barcoding of commercially important Grouper species (Perciformes, Serranidae) in the Philippines.

    PubMed

    Alcantara, Simon G; Yambot, Apolinario V

    2016-11-01

    Fish identification is generally challenging because of their unpronounced and overlapping morphological characters which is true in grouper species. In the Philippines, an updated, reliable and accurate inventory of this high value commercial groupers has not been carried out previously. Using molecular tools in the identification and inventory of fish species in the country is confined to few laboratories and experts in the country. In this study, 27 species of the Serranidae family were identified from the grouper samples collected from major fish landing sites and markets in the Philippines. The grouper species were molecularly identified using the cytochrome c oxidase I (COI) sequences for DNA barcoding. The accuracy of the inferred species-level taxonomy based on COI is supported with high similarity search (98-100%) both in BOLD and BLAST, well-distributed genetic distance values and cohesive clustering in the Neighbor-Joining Tree. Aside from reinforcing the classical methodology of grouper identification in the country, this pioneering study on molecular identification of Philippine groupers constitutes a significant contribution to the DNA barcode library of Philippine marine fishes and to the global barcode entries in general, which can be used when dealing with grouper taxonomy, biodiversity, stock assessment and trade. The results reveal the different localities where the grouper species can be possibly sourced out in the country for trade and aquaculture purposes. Several of the grouper species are included in the IUCN Red List of Threatened Species. As a tool for conservation ecology, this study signals the implementation of sustainable fisheries management regulation to protect in particular those which are listed under the IUCN.

  9. Complete mitochondrial genome of the sixblotch hind Cephalopholis sexmaculata (Pisces: Perciformes).

    PubMed

    Hsiao, Sheng-Tai; Chen, Kao-Sung; Tseng, Chen-Te; Wu, Chi-Lun

    2016-01-01

    The complete mitogenome of the sixblotch hind, Cephalopholis sexmaculata was presented in this study. This mitochondrial genome consists of 16,589 bp, with 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes, and a noncoding control region (CR), and its gene arrangement is identical to most vertebrates. The overall base composition of the heavy strand is A, 29.35%; G, 16.08%; C, 28.56%; and T, 26.01%. The COI gene started with GTG codon and the ATP6 gene started with CTG codon. The complete mitogenomic data may provide informative for further phylogenetic approach of species of Cephalopholis and related genera belong to the Epinephelidae groupers.

  10. Primary male development of two sequentially hermaphroditic groupers, Epinephelus akaara and Epinephelus awoara (Perciformes: Epinephelidae).

    PubMed

    Liu, M; Wang, Y-Y; Shan, X-J; Kang, B; Ding, S-X

    2016-04-01

    Gonad ontogeny of the Hong Kong grouper Epinephelus akaara (a bi-directional sex changer) and the yellow grouper Epinephelus awoara (a protogynous hermaphrodite) was examined for the first time from post-larval phase until first sexual maturation, by histology. Approximately 20 specimens of each species were collected randomly every 2-7 weeks from rearing tanks with natural sea water and temperature between June 2013 and June 2014. The paired gonadal primordia (GP) were observed at 6 weeks after hatching (wah) for both species; however, gonia were first observed in GP at 16 wah for E. akaara and at 8 wah for E. awoara. The timings for the appearance of primary-growth stage oocytes (O1) and the completion of ovarian lumen (OL) varied; both at 27 wah for E. akaara, and at 18 and 23 wah for E. awoara respectively. A bisexual-phase gonad with an OL, O1 and scattered spermatogenic cysts (SC) was observed at 27-29 wah for both E. akaara and E. awoara. Sexual differentiation was subsequently observed from the bisexual-phase gonad at 34 wah for E. akaara, and 41 wah for E. awoara, with the appearance of cortical-alveolus stage oocytes (O2) for developing female and the proliferation of SC for developing primary male (i.e. from juvenile directly). Ovaries of mature females contained the vitellogenic stage oocytes (O3) and scattered SC; testes of mature primary males had sperm in sperm sinuses within the gonadal wall and remained O1. Minimum age of first sexual maturation for both female and primary male of E. akaara was at 41 wah; minimum total length (LT ) of female (143 mm) was larger than that of primary male (137 mm L(T)). Minimum age and size of first sexual maturation for female of E. awoara (47 wah and 149 mm L(T), respectively) were larger than those of E. akaara. Developing primary males of E. awoara were found at 41-58 wah, however, mature males were not observed, indicating inconsistency in first sexual maturation for E. awoara. This study provided strong evidences of primary male pathway in E. akaara and E. awoara; the latter is confirmed to be diandric.

  11. Mitochondrial genome of the blackfin tuna Thunnus atlanticus Lesson, 1831 (Perciformes, Scrombidae).

    PubMed

    Márquez, Edna J; Isaza, Juan P; Alzate, Juan F

    2016-05-01

    Blackfin tuna, Thunnus atlanticus is a widespread epipelagic oceanic species in the western Atlantic. So far the mitochondrial genome of this species remained unknown, although the mitogenomes of all congeners are known. The mitochondrial genome encodes for 13 proteins, 21 tRNAs, 2 ribosomal RNAs and the gene synteny is conserved with other previously reported mitogenomes of tunas.

  12. Noise elicits hematological stress parameters in Mediterranean damselfish (Chromis chromis, perciformes): A mesocosm study.

    PubMed

    Vazzana, Mirella; Celi, Monica; Arizza, Vincenzo; Calandra, Giampiero; Buscaino, Giuseppa; Ferrantelli, Vincenzo; Bracciali, Claudia; Sarà, Gianluca

    2017-03-01

    In the last few decades, technological developments and the widespread rise of anthropic activities have increased the exposure of organisms to noise pollution, thus evoking great interest in its biological effects, particularly on the immune system. The aim of the present work was to investigate some of the biochemical parameters in the blood of Chromis chromis (Linnaeus, 1758) following in vivo exposure to noise levels of 200 and 300 Hz. Our results revealed that, compared to the control specimens, the fish exposed to noise had significantly increased levels of stress biomarkers such as glucose, lactate and total proteins in plasma, as well as a rise in the expression of heat shock protein 70 (HSP70).

  13. Temporal Dynamics of Reproduction of the Neotropical Fish, Crenicichla menezesi (Perciformes: Cichlidae)

    PubMed Central

    de Araújo, Andréa Soares; do Nascimento, Wallace Silva; Yamamoto, Maria Emília; Chellappa, Sathyabama

    2012-01-01

    The reproductive biology and the gonadal development cycle of the Neotropical cichlid fish, Crenicichla menezesi, is described. This species exhibits sexual dimorphism only during the spawning season. First sexual maturity of females is attained earlier than the males. Both macroscopic and histological investigations of ovaries and testes revealed four stages of gonadal maturation. Mean batch fecundity of females was 372 (±10,41) of mature oocytes. This species is a partial spawner, with an extended spawning period. Monthly values of GSI and the condition factor are negatively correlated during the gonadal development cycle of this species. PMID:22919339

  14. A new fish species of the subfamily Serraninae (Perciformes, Serranidae) from the Philippines.

    PubMed

    Williams, Jeffrey T; Carpenter, Kent E

    2015-01-19

    A new species of serranine fish is described from the Philippine Islands. A single specimen of a new species, Chelidoperca santosi, captured by fishermen working in Palawan waters was discovered in the public fish market in Iloilo City, Panay, Philippines. Two additional specimens of the new species, also from the Philippines, were subsequently discovered in the collections of the Museum Victoria, Australia. The new species is currently known only from the Philippines and is characterized by its distinctive coloration with a row of four small dark spots on the snout (two in front of each eye) and two dark spots on the chin (one on each side of the symphysis of the dentaries), a white anal fin with six large yellow spots separated by broad white interspaces and a narrow yellow distal border, caudal fin with narrow yellow bars and a yellowish distal margin and no dark spots, and a combination of meristic and morphological characters. 

  15. Endocrine disruption of phenanthrene in the protogynous dusky grouper Epinephelus marginatus (Serranidae: Perciformes).

    PubMed

    de Campos, Mariana Frias; Lo Nostro, Fabiana L; Da Cuña, Rodrigo H; Moreira, Renata Guimarães

    2017-06-23

    The dusky grouper Epinephelus marginatus is a protogynous hermaphrodite fish, that maintains high levels of plasma steroids as juveniles, as substrates for sex inversion. These fish are exposed to marine pollution from oil spills during cargo handling. Polycyclic aromatic hydrocarbons (PAHs), such as phenanthrene (Phe), are the main crude oil components and are toxic to fish, acting as endocrine disruptors (ED). This is the first study that investigated impacts of Phe as an ED in E. marginatus juveniles. An in vivo sublethal exposure (96h) to Phe was carried out at two concentrations (0.1mg/L and 1mg/L); exposure to the vehicle (ethanol; ETOH) was also performed. Plasma levels of 17β-estradiol (E2), testosterone (T) and 11-ketotestosterone (11-KT) were measured by ELISA. Gonads, liver and spleen were processed for histological analysis. In an in vitro bioassay, gonad fragments were incubated with Phe (8.91mg/L) or ETOH. Steroid levels in the culture media were measured by ELISA. The in vivo exposure to Phe triggered an increase of the area of the hepatocytes, increased number of melanomacrophagic centers and hemosiderosis in the spleen; ETOH induced similar effects on spleen. E2 and T levels did not change in plasma or in vitro media. In plasma, ETOH decreased 11-KT levels. Phenanthrene sharply reduced 11-KT levels in vitro. Although in vivo bioassay results were not unequivocal owing to ethanol effects, Phe might disrupt steroidogenesis in juvenile grouper, possibly causing dysfunctions during sex change and gonadal maturity, considering the importance of 11-KT in developing ovaries. Copyright © 2017 Elsevier Inc. All rights reserved.

  16. Diet-morphology correlations in the radiation of South American geophagine cichlids (Perciformes: Cichlidae: Cichlinae).

    PubMed

    López-Fernández, Hernán; Winemiller, Kirk O; Montaña, Carmen; Honeycutt, Rodney L

    2012-01-01

    Genera within the South American cichlid tribe Geophagini display specialized feeding and reproductive strategies, with some taxa specialized for both substrate-sifting and mouth brooding. Several lineages within the clade also possess an epibranchial lobe (EBL), a unique pharyngeal structure that has been proposed to have a function in feeding and/or mouth brooding. A recently published genus-level phylogeny of Neotropical cichlids was used as the evolutionary framework for investigating the evolution of morphological features presumably correlated with diet and mouth brooding in the tribe Geophagini. We tested for possible associations between the geophagine epibranchial lobe and benthic feeding and mouth brooding. We also addressed whether the EBL may be associated with unique patterns of diversification in certain geophagine clades. Tests of binary character correlations revealed the EBL was significantly associated with mouth brooding. We also tested for a relationship between diet and morphology. We analyzed stomach contents and morphometric variation among 21 species, with data for two additional species obtained from the literature. Principal Components Analysis revealed axes of morphological variation significantly correlated with piscivory and benthivory, and both morphology and diet were significantly associated with phylogeny. These results suggest that the EBL could be an adaptation for either feeding or mouth brooding. The EBL, however, was not associated with species richness or accelerated rates of phyletic diversification.

  17. Temporal dynamics of reproduction of the neotropical fish, Crenicichla menezesi (Perciformes: Cichlidae).

    PubMed

    de Araújo, Andréa Soares; do Nascimento, Wallace Silva; Yamamoto, Maria Emília; Chellappa, Sathyabama

    2012-01-01

    The reproductive biology and the gonadal development cycle of the Neotropical cichlid fish, Crenicichla menezesi, is described. This species exhibits sexual dimorphism only during the spawning season. First sexual maturity of females is attained earlier than the males. Both macroscopic and histological investigations of ovaries and testes revealed four stages of gonadal maturation. Mean batch fecundity of females was 372 (± 10,41) of mature oocytes. This species is a partial spawner, with an extended spawning period. Monthly values of GSI and the condition factor are negatively correlated during the gonadal development cycle of this species.

  18. A new species of the cardinalfish genus Siphamia (Perciformes, Apogonidae) from West Papua, Indonesia.

    PubMed

    Gon, Ofer; Allen, Gerald R; Erdmann, Mark V; Gouws, Gavin

    2014-11-06

    A new species of the cardinalfish genus Siphamia is described from specimens collected in the Province of West Papua, Indonesia, at depths of 50-72 m. Siphamia papuensis n. sp. has a striated light organ which makes it a member of the S. tubifer species group. Within this group it is closely related to S. argentea, sharing with the latter 13 pectoral-fin rays, 9 developed gill rakers and an irregular pattern of yellowish green bars on the body. It differs from S. argentea in having an incomplete lateral line and in lacking dark marks on the head, and at the origin and end of the dorsal-fin and anal-fin bases, as well as the absence of red spots along the light organ and along the back. Japanese records of S. tubulata are reidentified as S. argentea, and new locality records for S. argentea and S. stenotes are reported. Analysis of sequence data from a 16S rDNA fragment revealed the clear separation of S. papuensis n. sp., S. argentea and other included Siphamia species (S. jebbi, S. tubifer and S. stenotes). 

  19. Recovery of fat snook, Centropomus parallelus (Teleostei: Perciformes) after subchronic exposure to copper.

    PubMed

    Oss, Rodrigo Nunes; Baroni, Vinícius Dadalto; Duarte, Rafael Mendonça; Val, Adalberto Luis; Val, Vera Maria Fonseca Almeida; Gomes, Levy Carvalho

    2013-04-01

    We studied the recovery of juvenile fat snook (Centropomus parallelus) after subchronic exposure to different concentrations of copper. Healthy juveniles (1.98 g) were exposed to 25 or 50 μg Cu/L for 30 days (12 replicates with 5 fish in each one), and recovery was observed at 0, 4, 10, and 30 days after exposure (3 replicates with 5 fish in each one). Copper genotoxicity in exposed individuals was observed using a micronucleus assay, and recovery was not observed even 30 days post-exposure. Copper accumulation was observed in fish exposed to 25 or 50 μg/L of copper in the gills (14.4 and 34.4 μg/g, respectively) and muscle (5.7 and 5.5 μg/g, respectively), and a return to normal copper levels (6.0 μg/g for gills and 2.5 μg/g for muscle) was observed 4 and 30 days post-exposure in the gills and muscle tissues, respectively. Glutathione S-transferase (GST) was 80% inhibited in individuals exposed to copper and returned to normal levels for fish exposed to basal concentrations within 10 days. Although copper accumulation in tissues dispersed 30 days post-exposure, no recovery from genotoxicity was observed during this time. Thirty days was not enough to recover juvenile fat snook following subchronic exposure to copper. Copyright © 2013 Elsevier Inc. All rights reserved.

  20. Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus.

    PubMed

    Piazza, Yanina; Pandolfi, Matías; Da Cuña, Rodrigo; Genovese, Griselda; Lo Nostro, Fabiana

    2015-06-01

    Endosulfan (ES) is an organochlorine pesticide widely used in agriculture despite its high toxicity towards non-target organisms such as fish. It has been demonstrated that ES can cause negative effects on aquatic animals, including disruption of hormonal systems. However, the alterations produced by this pesticide on the reproductive axis of fish prior to sexual maturity, as well as possible modes of action have hardly been studied. This study aimed at assessing the effect of waterborne exposure to the pesticide ES on the reproductive axis during sexual differentiation of juveniles of the South American freshwater cichlid fish Cichlasoma dimerus. No mortality was observed due to ES subchronic exposure (90 days post-fertilization). Exposure to ES did not affect body weight nor morphometric parameters, indicating that larvae nutritional state was not affected. Timing of sexual differentiation, gonadal morphology and sex ratio were likewise not altered by ES. However, ES acted as an endocrine disrupting chemical in this species as the morphometry of gonadotropin-releasing hormones (GnRH) producing cells was altered. Exposure to ES altered nuclear area, cell area and nucleus/cytoplasm ratio of GnRH II neurons, and cell and nuclear area and diameter of GnRH III neurons. Interestingly, in our previous study, exposure before sex differentiation (30 day exposure) caused no alteration to GnRH II and III, and did alter GnRH I and FSH cells. These alterations could lead to changes in circulating hormone levels, especially when fish are exposed for prolonged periods, ultimately impairing reproductive fitness. C. dimerus juveniles can be an interesting biological model to perform toxicological studies with the intent to assess early disruption endpoints in the reproductive axis during development.

  1. Reproductive biology of Oreochromis niloticus (Perciformes: Cichlidae) at Emiliano Zapata dam, Morelos, Mexico.

    PubMed

    Peña-Mendoza, B; Gómez-Márquez, J L; Salgado-Ugarte, I H; Ramirez-Noguera, D

    2005-01-01

    The reproductive season of tilapia was studied by monthly samplings at Emiliano Zapata dam, Morelos State, Mexico. From February 1999 through February 2000 a sample of 50 fish was taken from the commercial catch (castnet, 6.5 cm of mesh size). The observed sex ratio was 1:1.29 (females:males) (chi(2)=10.26; p<0.05). The tilapia reached maturity at 151.3 mm (females) and 152.0 mm (males) of total length. Rainy (August) and dry (February) seasons were determined as the breeding period. Fecundity variation was better correlated with length (r=0.7473: p<0.002) than with weight (r=0.7395; p<0.002). The fecundity ranged between 243 and 847 oocytes per fish, with egg diameter from 300 to 3 700 microm. Intensive breeding activity in August and February coincide with phytoplankton biomass increase.

  2. Fine structure of spermatozoa in the common pandora (Pagellus erythrinus Linnaeus, 1758) (Perciformes, Sparidae).

    PubMed

    Maricchiolo, G; Genovese, L; Laurà, R; Micale, V; Muglia, U

    2004-10-01

    Scanning and transmission electron microscopy were used to investigate the fine structure of the sperm of the Sparid fish Pagellus erythrinus L. The spermatozoon of pandora has a spherical head lacking an acrosome, a cone-shaped midpiece and a long tail. The midpiece houses a single mitochondrion. The centriolar complex lies inside the nuclear fossa and is composed of a proximal and a distal centriole which are arranged at right angles to each other. The flagellum is inserted medio-laterally into the head, contains the conventional 9+2 axoneme and possesses one pair of lateral fins. On the basis of its ultrastructural organization, the pandora sperm can be regarded as an evolved form of the primitive spermatozoon found in Teleosts. According to the morphological classification proposed by Mattei (1970), the sperm of pandora belongs to a "type I" designation, like that of the other Sparid fish.

  3. Metazoan parasites of blue jack mackerel Trachurus picturatus (Perciformes: Carangidae) from Portuguese mainland waters.

    PubMed

    Hermida, M; Pereira, A; Correia, A T; Cruz, C; Saraiva, A

    2016-07-01

    Blue jack mackerel, Trachurus picturatus, is a carangid fish which constitutes an important commercial resource in the north-east Atlantic. Its metazoan parasite community from Portuguese mainland waters was investigated here for the first time. Nine parasite taxa were found, most of which are common parasites of Trachurus spp. The parasite community was broadly similar to that of the Atlantic horse mackerel, T. trachurus, from the same region, but two digenean species were detected in blue jack mackerel, Monascus filiformis and Tergestia sp., which did not occur in horse mackerel from this region. A comparison with the two previous studies of T. picturatus parasite communities shows that continental-shelf regions are characterized by higher prevalences of digenean trematodes and an absence of trypanorhynch cestodes, in contrast with oceanic regions.

  4. Kudoa saudiensis sp. n. (Myxosporea: Multivalvulida) infecting oocytes of the Indian mackerel Rastrelliger kanagurta (Perciformes: Scombridae).

    PubMed

    Mansour, Lamjed; Harrath, Abdel Halim; Abdel-Baki, Abdel-Azeem S; Al-Quraishy, Saleh; Al, Suliman Y Omar

    2015-01-01

    During a survey the occurrence of Kudoa quraishii Mansour, Harrath, Abd-Elkader, Alwasel, Abdel-Baki et Al Omar, 2014, recently identified in the muscles of the Indian mackerel, Rastrelliger kanagurta (Cuvier), a species of Kudoa Meglitsch, 1947 infecting oocytes of mature females of the same host fish was found. The new species, for which the name Kudoa saudiensis sp. n. is proposed, infects oocytes that are enlarged with a whitish colour. The parasite develops in vesicular polysporous plasmodia within the oocyte. Infection occurs with a mean prevalence of 20% (7/35) of examined females. Mature spores are quadratic in shape in apical view, having four equal valves and four symmetrical polar capsules. Fresh spores are 2.4-3.6 µm long (mean ± SD 3.1 ± 0.3 µm), 4.3-5.4 µm (4.7 ± 0.3 µm) wide and 3.4-4.3 µm (3.8 ± 0.3 µm) in thickness and long. The smaller size of the new Kudoa species was the distinctive feature that separates it from all previously described species. Molecular analysis based on the SSU rDNA sequences shows that the highest percentage of similarity of 98.5% was observed with K. ovivora Swearer et Robertson, 1999, reported from oocytes of labroid fish from the Caribbean coasts of Panama. The percentage of similarity was 98% with K. azevedoi Mansour, Thabet, Chourabi, Harrath, Gtari, Al Omar et Ben Hassine, 2013 and 89% with K. quraishii. Phylogenetic analysis of the SSU and LSU rDNA data revealed a consistent of the new species with K. azevedoi and K. ovivora. Our findings support the creation of Kudoa saudiensis sp. n. that infects oocytes of the Indian mackerel Rastrelliger kanagurta.

  5. Growth and mortality rates of bigeye tuna Thunnus obesus (Perciformes: Scombridae) in the central Atlantic Ocean.

    PubMed

    Zhu, Guoping; Xu, Liuxiong; Zhou, Yingqi; Chen, Xinjun

    2009-01-01

    Age and growth parameters were estimated for bigeye tuna Thunnus obesus Lowe, 1839 sampled from China longline fisheries in the central Atlantic Ocean from October 2002 to July 2003 and from August 2004 to March 2005. The von Bertalanffy growth parameters were estimated at L(infinity)=217.9 cm fork length, k=0.23 year(-1), and t(0)=-0.44 year. The total mortality rate (Z) was estimated to be from 0.82 to 1.02, the fishing mortality (F) and the natural mortality were 0.54 year(-1) and 0.39 year(-1), respectively. The exploitation ratio (E) was 0.35. This study provides the detailed estimates of growth and mortality rate for bigeye tuna in the central Atlantic Ocean, which can be used as biological input parameters in further stock evaluations in this region. However, age analysis, additional validation of the size composition and stock structure are needed for future studies.

  6. 50 CFR 665.201 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... stripe snapper Lutjanus kasmira. kalekale yellowtail snapper Pristipomoides auricilla. `pakapaka pink snapper Pristipomoides filamentosus. kalekale pink snapper Pristipomoides seiboldii. gindai...

  7. 50 CFR 665.201 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... stripe snapper Lutjanus kasmira. kalekale yellowtail snapper Pristipomoides auricilla. `pakapaka pink snapper Pristipomoides filamentosus. kalekale pink snapper Pristipomoides seiboldii. gindai...

  8. 50 CFR 665.201 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... stripe snapper Lutjanus kasmira. kalekale yellowtail snapper Pristipomoides auricilla. `pakapaka pink snapper Pristipomoides filamentosus. kalekale pink snapper Pristipomoides seiboldii. gindai...

  9. 50 CFR 665.201 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... stripe snapper Lutjanus kasmira. kalekale yellowtail snapper Pristipomoides auricilla. `pakapaka pink snapper Pristipomoides filamentosus. kalekale pink snapper Pristipomoides seiboldii. gindai...

  10. 50 CFR 665.201 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... stripe snapper Lutjanus kasmira. kalekale yellowtail snapper Pristipomoides auricilla. `pakapaka pink snapper Pristipomoides filamentosus. kalekale pink snapper Pristipomoides seiboldii. gindai...

  11. Biogeography of tropical Indo-West Pacific parasites: a cryptic species of Transversotrema and evidence for rarity of Transversotrematidae (Trematoda) in French Polynesia.

    PubMed

    Cribb, Thomas H; Adlard, Robert D; Bray, Rodney A; Sasal, Pierre; Cutmore, Scott C

    2014-04-01

    We sought transversotrematid trematodes from French Polynesian fishes by examining 304 individual scaled fishes of 53 species from seven families known to harbour the family elsewhere. A single species was found at two locations in the Tuamotus Archipelago on two species of Chaetodontidae (Chaetodon auriga and Chaetodon ephippium) and one species of Lutjanidae (Lutjanus gibbus). The species closely resembles Transversotrema borboleta Hunter & Cribb, 2012 from chaetodontids and lutjanids of the northern Great Barrier Reef (GBR) but differs from it consistently in 8 base positions of ITS2 rDNA. This level of variation exceeds that between some clearly morphologically distinct pairs of species of Transversotrema and the form from French Polynesia is thus interpreted as a distinct, though cryptic, species and named Transversotrema polynesiae n. sp. The new species forms part of a complex of species, here characterised as the T. borboleta complex, associated with chaetodontids and lutjanids in the tropical Indo-West Pacific. Most of the putative species within this complex are yet to be described. Comparison of identical numbers of matched samples of fishes from French Polynesia, Heron Island (southern GBR) and Lizard Island (northern GBR) revealed 1, 4 and 10 species of Transversotrema respectively suggesting that the French Polynesian fauna is depauperate for this family. In addition to those species apparently missing from suitable hosts in French Polynesia, several species from further west infect fishes (especially Nemipteridae) that are themselves absent from French Polynesia. This dramatic east-west decline in richness contrasts strongly with what is known for monogeneans, which appear to maintain their richness over the same scale, and is more precipitate than is known for other groups of trematodes. The decline might be explained in part by the absence of the as yet unknown first intermediate hosts in French Polynesia. However, we predict that it is explained

  12. Four new species of the Indo-Burmese genus Badis from West Bengal, India (Actinopterygii: Perciformes: Badidae).

    PubMed

    Valdesalici, Stefano; Voort, Stefan Van Der

    2015-07-13

    Four new species of Badis are described from West Bengal, India. Badis andrewraoi, new species, is diagnosed by a combination of characters which include absence of a black caudal-fin margin, absence of a conspicuous dark blotch on pectoral-fin base, absence of cleithral and opercular blotches, absence of a blotch on the dorsolateral aspect of the caudal peduncle, absence of an ocellus on the caudal-fin base, presence of a conspicuous median black caudal peduncle blotch, a medially broader posterior-most bar, displaying as a partially absorbed second median caudal peduncle blotch, presence of an additional bar anteriorly, and vertical bars restricted to lower half of body and never forming large blocks. Badis autumnum, new species, is diagnosed by a combination of characters which include two autapomorphies: presence of a blotch above the base of the opercular spine, and of a conspicuous dark blotch on pectoral-fin base. Additionally, it presents a black caudal-fin margin, outlining entire fin, absence of a cleithral blotch, absence of a blotch on the dorsolateral aspect of the caudal peduncle, absence of an ocellus on the caudal-fin base, presence of a conspicuous median black caudal peduncle blotch, a medially broader posterior-most bar, displaying as a partially absorbed second median caudal peduncle blotch, and presence of an additional bar anteriorly. Badis kyanos, new species, is diagnosed by a combination of characters which include absence of a conspicuous dark blotch on pectoral-fin base, absence of cleithral and opercular blotches, absence of a blotch on the dorsolateral aspect of the caudal peduncle, absence of an ocellus on the caudal-fin base, presence of a conspicuous median black caudal peduncle blotch, a medially broader posterior-most bar, displaying as a partially absorbed second median caudal peduncle blotch, presence of an additional bar anteriorly, its vertical bars forming large, fragmented black blocks dorsolaterally and ventrolaterally, and a unique stress colouration consisting of a dark grey body, metallic dark blue operculum, flanks almost entirely devoid of bars, large, fragmented black blocks dorsolaterally, and absence of a black caudal-fin margin. Based on similarities in colour pattern and biometrics they are deduced to be closely related and form a new species group, which we herein define. The three species are divergent and diagnosable from each other and represent valid evolutionary species. The fourth species represents a cryptic unit that was heretofore unrecognised and considered as the most widespread species of the genus, B. badis. We describe this lineage as Badis soraya, new species, and find it belongs to the B. badis species group. It can be diagnosed by a combination of characters which include absence of opercular blotches, absence of a blotch on the dorsolateral aspect of the caudal peduncle, absence of an ocellus on the caudal-fin base, presence of a cleithral blotch, presence of a small oval black blotch medially on caudal peduncle, interorbital width 6.3-8.8 % SL, dorsal-fin spines XIV-XVI, scales in lateral row 25-27, and vertebrae number 27.

  13. [Variations of the infracommuity during the ontogeny of Chromis cyanea (Perciformes: Pomcentride) in the North coast of Havana, Cuba].

    PubMed

    Fernández Osorio, Ramón Alexis; Corrada Wong, Raúl Igor; Armenteros, Maickel

    2015-09-01

    The infracommunty or parasites or unromis cyanea (Pisces: Pomacentridae) was studied along the ontogenetic development in the North coast of Havana, Cuba. The objectives were: a- to prove that the core species appears before the strange and stochastic species and they are responsible for the structure in the infracommunity, b- to determine if there is a relationship among the ecological describers of the parasitic infracommunity with the total length. A total of 278 specimens of C. cyanea were captured during the dry season (April) of 2010. The body size range was from 1.5 to 11.5 cm including juvenile and adults. We collected 2 861 parasite specimens belonging to 20 taxa: Crustacea (5), Nematoda (5), Trematoda (4), Cestoda (2), Monogenea (2), Turbellaria (1) and Ciliophora (1). The taxa Tetraphyllidea and Anilocra chromis constituted the core of the parasitic infracommunity. The sequence of appearance and persistence of these taxa during the ontogenetic development, supported the hypothesis of the core species. The changes in the infracommunity, from 6 cm body size, could be the result of an accumulative effect combined with changes of the diet that caused the ingestion of new parasite infective stages. We concluded that the ontogenetic development of C. cyanea constitutes an important variable in the formation of the parasitic infracommunity.

  14. Description of a new species and redescriptions of two rare species of Parapercis (Perciformes: Pinguipedidae) from the tropical Pacific Ocean.

    PubMed

    Ho, Hsuan-Ching

    2015-08-10

    Parapercis johnsoni sp. nov. is described based on 19 specimens from Marquesas Islands, French Polynesia. It differs from congeners in having a combination of the following characters: dorsal-fin rays V, 21; anal-fin rays I, 17; pectoral-fin rays modally 17; pored lateral-line scales modally 52 or 53; predorsal scales 7 or 8; transverse scale rows 3.5 or 4 + 14 or 15; total gill rakers on 1st gill arch 13-16; single row of teeth on vomer; 6 large canines at front of lower jaw; and a distinct coloration. Two rare species, P. flavescens Fourmanoir & Rivaton, 1979 and P. fuscolineata Fourmanoir, 1985, are redescribed based on the types and newly identified specimens. Comments on other species occurring in the area are provided.

  15. [Embryonic and Larval Development of the Asian Seabass Lates calcarifer (Pisces: Perciformes: Latidae) under Thermostatically Controlled Conditions].

    PubMed

    Shadrin, A M; Pavlov, D S

    2015-01-01

    Material for this study was obtained from the hatchery with brood stock of Lates calcarifer that originated from a natural population living in inshore waters off Central Vietnam. Commercial interest in L. calcarifer as an object of mariculture and wildstock fishery has resulted in several publications on its early life history; nevertheless, comprehensive description of early development of L. calcarifer based on controlled incubation of embryos and larvae has remained absent. In the present paper embryonic and larval development to the stage of anlage of pelvic fins is described in detail and illustrated with original drawings of live material on the basis of thermostatically controlled incubation of embryos at 27°C and larvae at 26.8°C (26.5-28.0°C). The first cleavage furrow appeared at the age of 33.5 min. The duration of synchronous cleavage cycle was 16 min. About 80% of all embryos hatched at the age of 18 h. The length of newly hatched larva during the first hour after emergence from the egg shell was 1.63 ± 0.016 mm (1.50-1.75 mm). Chronology of development of the organs, early circulatory system, and pigmentation pattern is given. The dynamics of change in the trunk and caudal body segment number in larva from hatching to the moment of anlage of pelvic fins is shown. The total number of body segments reached the maximum value of 26-27 soon after hatching and then decreased to 20-21 segments. Newly received data are discussed in a comparative context of development of some other teleosts.

  16. Molecular systematics and taxonomic status of three latitudinally widespread nototheniid (Perciformes: Notothenioidei) fishes from the Southern Ocean.

    PubMed

    Miya, Tshoanelo; Gon, Ofer; Mwale, Monica; Poulin, Elie

    2016-01-08

    The taxonomic status of the three nototheniids, Lepidonotothen squamifrons, L. larseni and Gobionotothen marionensis from different localities in the Southern Ocean is re-evaluated at the DNA level. DNA sequence divergences and phylogenetic relationship were estimated using a combined mitochondrial (mtDNA, ND2 and COI) dataset and data for one nuclear gene (S7 intron 1). Phylogenies of both datasets had Lepidonotothen kempi nested within L. squamifrons lineage, with low sequence divergences (0% to 0.4%) between the two nominal species suggesting that they are populations of one species. Therefore, these results do not support the previous splitting of L. squamifrons into different species. Similarly, the L. larseni specimens also represented a single genetic unit (0.3% to 0.6%) with low geographic variation between Atlantic and Indian Ocean specimens, which does not support the splitting of this species into geographically restricted species. The mtDNA phylogeny clearly separated individuals of G. acuta from Kerguelen, Heard and MacDonald Islands from G. marionensis individuals into different clades, with sequence divergence of 2.9% between these clades supporting they are different species.

  17. Cotylogaster basiri Siddiqi & Cable, 1960 (Aspidobothrea: Aspidogastridae) in Pogonias cromis (Linnaeus, 1766) (Perciformes: Sciaenidae): first report from Brazil.

    PubMed

    Gallas, M; Silveira, E F; Périco, E

    2016-07-11

    Up until now, there have been few studies of the helminth fauna of Brazilian populations of Pogonias cromis. In North America, P. cromis is known to be a definitive host of Cotylogaster basiri, although there have been no reports of any species of Cotylogaster infecting fish in Brazil. During necropsies of marine fish obtained from professional fisherman in the state of Rio Grande do Sul, Brazil, specimens of C. basiri were found in the intestine and rectum of P. cromis. In comparison with previous studies, differences were found in relation to the number of lobes in the buccal disc and the development of the biocellate larvae in the eggs of C. basiri. This is the first report of C. basiri in P. cromis from Brazil, contributing to the understanding of the helminth fauna of this fish from the coastal zone of Brazil.

  18. Comparative Mitogenomics of the Genus Odontobutis (Perciformes: Gobioidei: Odontobutidae) Revealed Conserved Gene Rearrangement and High Sequence Variations.

    PubMed

    Ma, Zhihong; Yang, Xuefen; Bercsenyi, Miklos; Wu, Junjie; Yu, Yongyao; Wei, Kaijian; Fan, Qixue; Yang, Ruibin

    2015-10-20

    To understand the molecular evolution of mitochondrial genomes (mitogenomes) in the genus Odontobutis, the mitogenome of Odontobutis yaluensis was sequenced and compared with those of another four Odontobutis species. Our results displayed similar mitogenome features among species in genome organization, base composition, codon usage, and gene rearrangement. The identical gene rearrangement of trnS-trnL-trnH tRNA cluster observed in mitogenomes of these five closely related freshwater sleepers suggests that this unique gene order is conserved within Odontobutis. Additionally, the present gene order and the positions of associated intergenic spacers of these Odontobutis mitogenomes indicate that this unusual gene rearrangement results from tandem duplication and random loss of large-scale gene regions. Moreover, these mitogenomes exhibit a high level of sequence variation, mainly due to the differences of corresponding intergenic sequences in gene rearrangement regions and the heterogeneity of tandem repeats in the control regions. Phylogenetic analyses support Odontobutis species with shared gene rearrangement forming a monophyletic group, and the interspecific phylogenetic relationships are associated with structural differences among their mitogenomes. The present study contributes to understanding the evolutionary patterns of Odontobutidae species.

  19. Ecology and management of the invasive lionfish Pterois volitans/miles complex (Perciformes: Scorpaenidae) in Southern Costa Rica.

    PubMed

    Sandel, Vera; Martínez-Fernández, Damián; Wangpraseurt, Daniel; Sierra, Luis

    2015-03-01

    Invasive species alter ecosystem integrity and functioning and are considered one of the major threats to biodiversity on a global scale. The indopacific lionfish (Plerois volitans [Linnaeus, 1758] / miles [Bennet, 1882] complex) is the first non-native marine fish that has established itself in the Western Atlantic. It was first reported in Florida in the 1980s and then spread across the entire Caribbean in subsequent years. In Costa Rica, lionfish were first sighted by the end of 2008 and are now present in all South Caribbean reefs. Lionfish are a major problem for local fisherman by displacing native fish species. The aim of this study was to determine population density, size and diet of lionfish populations at four study sites along the Southern Caribbean coast of Costa Rica. Two of the sites were located inside the National Park Cahuita where regular lionfish removal occurs, whereas the other two study sides do not experiment this kind of management. Total length and wet weight of >450 lionfish individuals were determined between March and June 2011. Three relative metrics of prey quantity (percent number, percent frequency, and percent weight) were compared from approximately 300 lionfish caught with the polespear in shallow waters (<7 m depth). Population density was assessed weekly through visual transect surveys. Our results showed that lionfish preyed mostly upon teleosts and crustaceans. Teleosts dominated lionfish diet in percent frequency (71%) and percent weight (85%), whereas crustaceans had the highest percent number (58%). The top five teleost families of dietary importance were Pomacentridae, Acanthuridae, Blennidae, Labridae and Serranidae. The average total length (+/- SD) of lionfish was 18.7 (+/- 5.7)cm and varied significantly between sites (p<0.001). Mean density of lionfish was 92fish/ha with no significant differences between sites. Smallest fish and lowest densities were found at the two sites inside the National Park Cahuita. Despite management efforts on a regional scale, nationwide efforts are ineffective and lionfish control activities are poorly implemented. We conclude that there is an urgent need to develop an improved institutional framework for local lionfish control that promotes effective coordination among the relevant stakeholders in order to deal with invasive lionfish in Costa Rica.

  20. A multi-gene dataset reveals a tropical New World origin and Early Miocene diversification of croakers (Perciformes: Sciaenidae).

    PubMed

    Lo, Pei-Chun; Liu, Shu-Hui; Chao, Ning Labbish; Nunoo, Francis K E; Mok, Hin-Kiu; Chen, Wei-Jen

    2015-07-01

    Widely distributed groups of living animals, such as the predominantly marine fish family Sciaenidae, have always attracted the attention of biogeographers to document the origins and patterns of diversification in time and space. In this study, the historical biogeography of the global Sciaenidae is reconstructed within a molecular phylogenetic framework to investigate their origin and to test the hypotheses explaining the present-day biogeographic patterns. Our data matrix comprises six mitochondrial and nuclear genes in 93 globally sampled sciaenid species from 52 genera. Within the inferred phylogenetic tree of the Sciaenidae, we identify 15 main and well-supported lineages; some of which have not been recognized previously. Reconstruction of habitat preferences shows repeated habitat transitions between marine and euryhaline environments. This implies that sciaenids can easily adapt to some variations in salinity, possibly as the consequence of their nearshore habitats and migratory life history. Conversely, complete marine/euryhaline to freshwater transitions occurred only three times, in South America, North America and South Asia. Ancestral range reconstruction analysis concomitant with fossil evidence indicates that sciaenids first originated and diversified in the tropical America during the Oligocene to Early Miocene before undergoing two range expansions, to Eastern Atlantic and to the Indo-West Pacific where a maximum species richness is observed. The uncommon biogeographic pattern identified is discussed in relation to current knowledge on origin of gradients of marine biodiversity toward the center of origin hypothesis in the Indo-West Pacific.

  1. Structure comparison of control region in Stromateoidei and complete mitochondrial genome of the bluefin driftfish Psenes pellucidus (Perciformes, Nomeidae).

    PubMed

    Wei, Tao; Zhang, Bo; Sun, Yuena

    2014-10-01

    In the present study, the complete mitochondrial genome of the bluefin driftfish Psenes pellucidus was determined first. The genome was 16,572 bp in length and consisted of 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA genes and 2 main non-coding regions. The mitochondrial genome of bluefin driftfish had common features about gene arrangement, base composition and tRNA structures compared with those of most of the bony fishes. In the control region, a termination-associated sequence, the central conserved block (CSB-F, CSB-E and CSB-D) and the conserved sequence blocks (CSB-1, CSB-2 and CSB-3) were determined. Meanwhile, the conserved motif 5'-GCCGG-3' was identified in the origin of light strand replication of bluefin driftfish. This mitogenome sequence data would play key role in phylogenetic analysis of Stromateoidei.

  2. A review of the genus Pempheris (Perciformes, Pempheridae) of the Red Sea, with description of a new species.

    PubMed

    Koeda, Keita; Yoshino, Tetsuo; Imai, Hideyuki; Tachihara, Katsunori

    2014-04-30

    Four species of the fish genus Pempheris are recognized for the Red Sea: P. adusta Bleeker, 1877; P. mangula Cuvier, 1829; P. nesogallica Cuvier in Cuvier & Valenciennes, 1831; and a new species P. tominagai. All are wide-ranging in the western Indian Ocean, and P. mangula has migrated via the Suez Canal to the eastern Mediterranean Sea. Morphological and genetic analysis of 15 species in this genus show that P. adusta, a widely distributed species, that can't be divided into different species, because of the continuity of morphologies and distribution, and lack of variance in genetics between Indian Ocean, Red Sea, and Pacific Ocean populations. This confirms that the two subspecies described by Randall et al. (2013) are both synonyms of P. adusta. Pempheris adusta is distinguished from other species by a blackish spot on pectoral fin base, pored lateral-line scales 56-64, scale rows above lateral line 4 1/2-6 1/2, distinct blackish band on outer edge of anal fin, and blackish band on posterior edge of caudal fin. Pempheris mangula was named by Cuvier (1829) in a footnote making reference to a drawing and short description in Russell (1803) of a Pempheris from southeast India, giving only the native name ''Mangula-Kutti'', and listing no specimen. The wide distribution of this species, from the Indian Ocean to the Red Sea is also demonstrated by morphological and genetic analysis. Thus, the specimen collected from southern India is herein designated as the neotype. This species is distinguished from other species by its huge eye, deep body, blackish tip of the dorsal fin, pored lateral-line scales 49-60, and scale rows above lateral line 4 1/2-5 1/2. The extant syntype of Kossmann & Räuber's P. rhomboidea is designated as the lectotype of the species; however, P. rhomboidea is a synonym of P. mangula. In addition, Kossmann & Räuber's Pempheris erythraea and P. russellii Day, 1888 are also synonyms of P. mangula. Of two existing syntypes of P. nesogallica from Mauritius, one is designated as the lectotype, the other is re-identified as P. mangula; P. nesogallica is presently known only from the southern Red Sea. This species has a similar morphology to P. mangula, but can be distinguished by a smaller eye than P. mangula, and lack irregular faint longitudinal light stripes on the body side. Pempheris tominagai are distinguished from P. schwenkii Bleeker 1855, formerly misidentified, by the form of posterior nostril, scale counts, color of caudal fin, and by a 2.1% mitochondrial DNA sequence divergence. 

  3. Two new species of Cabillus (Perciformes: Gobiidae) and the first record of Cabillus macrophthalmus from the Western Indian Ocean.

    PubMed

    Kovačić, Marcelo; Bogorodsky, Sergey V

    2013-01-01

    Two new species of the gobiid genus Cabillus, C. nigromarginatus sp. nov. and Cabillus nigrostigmus sp. nov. are described. Cabillus nigromarginatus (from Rodrigues, Western Indian Ocean) is distinguished from congeners by having 18-20 pectoral-fin rays; predorsal area naked; two scales with enlarged ctenii ventrally and dorsally at the caudal-fin base; head with anterior and posterior oculoscapular, and preopercular canals, with pores σ, λ, κ, ω, α, β, ρ, ρ1, ρ2 and γ, δ, ε respectively; the body with four midline lateral blotches, with two or three of them expanding upwards in dorsal saddles; a dark triangular blotch at caudal-fin base; and predorsal with pigmentation at lateral edges forming a rectangle. Cabillus nigrostigmus (from the Red Sea) is distinguished from its congeners by having 19 pectoral-fin rays; transverse scale series 7; nape scaled, median predorsal scales 7; body depth 5.8-6.0 in SL; snout length 1.5-1.9 in eye diameter; caudal-peduncle depth in its length 2.4-2.5; a broad dark brown bar below first dorsal fin beginning anteriorly at the level of fourth spine of the first dorsal fin; elongate black blotch along posterior half of first dorsal fin extending into the sixth spine and adjacent membranes; and midlateral black spot at the end of caudal peduncle followed by S-shaped dark bar. Cabillus macrophthalmus is recorded for the first time in the Western Indian Ocean (Red Sea and Seychelles) and redescribed.

  4. Redescription of Parapercis okamurai Kamohara, 1960 (Perciformes: Pinguipedidae), based on specimens newly collected from Taiwan and Japan.

    PubMed

    Ho, Hsuan-Ching

    2014-09-01

    A rare species of sandperch, Parapercis okamurai, is redescribed based on 2 types and 15 specimens newly collected from Taiwan and Japan. The species is unique in having cycloid scales on the parietal, opercle and subopercle, except for few large ctenoid scale that covers the base of the opercular spine; body color yellowish dorsally, with 10 or 11 faint yellow bands on lateral body and pale ventrally; and black spots on inner side of upper pectoral fin base. It can also be distinguished from congeners by having a combination of the following characters: dorsal-fin rays V, 23; anal-fin rays I, 19; pectoral-fin rays 18; pored lateral-line scales 59-64; medial predorsal scales 9-10; transverse scale rows 4.5-5.5/14-15; circumpeduncular scales 20-21; gill raker4-5+9-10=13-16; -4 pairs of canine teeth at front of lower jaw; 2-4 rows of teeth on vomer; 2 rows of teeth on palatines; scales on cheek moderately large, each half embedded; and body color yellowish dorsally and a prominent ocellus at upper caudal-fin base.

  5. [IDENTIFICATION OF PHOSPHOETHANOLAMINE AND PHOSPHOSERINE IN THE BRAIN OF POND FISH PERCCOTTUS GLEHNI (ELEOTRIDAE, PERCIFORMES, DYB. 1877)].

    PubMed

    Karanova, M V

    2015-04-01

    Considerable modifications of the pools of free amino acid and some other compounds in the body ectothermic animals in response to the decrease in temperature are a consequence of the complexity of their adaptation mechanisms. There is no data in the literature on the contribution of such free connections in the low-temperature adaptation of brain ectothermic animals. In a previous study, it was found that as a result of exposure to acute cold shock in the brain eurothermal fish in large numbers appear two ninhydrinpositive compounds of unknown nature. In this paper, found that by the beginning of the winter period in the brain are accumulated the same connection also. Found that these are metabolites of phospholipids, phosphoethanolamine and phosphoserine. Phosphoethanolamine pool 94 times more in winter than in summer, and phosphoserine is only in the winter. Assumes that the accumulation of phosphoethanolamine and phosphoserine related to adaptation and modification of membrane phospholipid at low temperatures.

  6. A new species of Coryogalops (Perciformes: Gobiidae) and the first adult record of Feia nympha from the Red Sea.

    PubMed

    Kovačić, Marcelo; Bogorodsky, Sergey V; Mal, Ahmad O

    2016-03-31

    A new species of the gobiid genus Coryogalops, C. nanus sp. nov. is described from the Red Sea. Coryogalops nanus sp. nov. is distinguished from congeners in having dorsal-fin rays VI + I,10; anal-fin rays I,9; pectoral-fin rays 12-14 (lowest count known for the genus), two upper rays with free tips; pelvic fins I,5, joined to form an emarginate disc, no pelvic frenum; predorsal area and narrow area at base of first dorsal fin naked; longitudinal scale series 26-29; transverse scale rows counted forward 6-7; transverse scale rows counted backward 7-8; circumpeduncular scale rows 11-12; seven transverse suborbital rows of sensory papillae; no tentacle above upper eye margin; anterior nostril tubular, without flap at its tip, posterior nostril pore-like; body semitranslucent, covered with small scattered orange-yellow spots and speckles, those in predorsal area contain black dots; an internal row of white spots along ventral part of body above anal-fin base and on caudal peduncle; head with small scattered orange to yellowish brown spots; first dorsal fin with two broad white bands at base of fin and distally, and with hyaline area densely dotted with melanophores in the middle of fin; preserved specimens opaque white to yellowish with sparse melanophores. An adult specimen of Feia nympha is recorded for the first time in the Red Sea and the lateral line system of this species is described.

  7. A New Record for Occurrence of Symphodus bailloni (Osteichthyes: Perciformes: Labridae) in the Western Black Sea Coast of Turkey

    PubMed Central

    Göktürk, Didem; Karakulak, F. Saadet; Ünsal, Nuran; Kahraman, Abdullah E.

    2012-01-01

    The fish species Symphodus bailloni (Valenciennes, 1839) reported in the present study were collected between June 2010 and June 2011 from the western Black Sea coasts which were previously not recorded from the Black Sea coast of Turkey. A total of 717 specimens of S. bailloni were measured, ranging between 8.9 and 15.4 cm TL. Morphometrics, meristics, and diagnostic characteristics of the species are presented. PMID:22593703

  8. A complex of species related to Paradiscogaster glebulae (Digenea: Faustulidae) in chaetodontid fishes (Teleostei: Perciformes) of the Great Barrier Reef.

    PubMed

    Diaz, Pablo E; Bray, Rodney A; Cutmore, Scott C; Ward, Selina; Cribb, Thomas H

    2015-10-01

    A total of 1523 individuals of 34 species of chaetodontids from the Great Barrier Reef were examined for faustulid trematodes. Specimens resembling Paradiscogaster glebulae Bray, Cribb & Barker, 1994 were found in nine chaetodontid species at three localities. These specimens are shown, on the basis of combined morphological and molecular analyses, to comprise a complex of morphologically similar and partly cryptic species. The complex may comprise as many as six distinct species of which three are resolved here. The true P. glebulae is identified in Chaetodon ornatissimus Cuvier, 1831, Chaetodon aureofasciatus Macleay, 1878, Chaetodon plebeius Cuvier, 1831, Chaetodon rainfordi McCulloch, 1923 and Chaetodon speculum Cuvier, 1831. Two new species are described, Paradiscogaster munozae n. sp. from Heniochus varius (Cuvier, 1829), Heniochus chrysostomus Cuvier, 1831 and Chaetodon citrinellus Cuvier, 1831 and Paradiscogaster melendezi n. sp. from Chaetodon kleinii Bloch, 1790. In terms of morphology the three species differ most clearly in the development of the appendages on the ventral sucker. The three species differ at 3-6consistent bp of ITS2 rDNA. The host-specificity of the three species differs strikingly. P. melendezi n. sp. infects just one fish species, P. glebulae infects species of only one clade of Chaetodon, and P. munozae n. sp. infects quite unrelated species. The basis of this unusual pattern of host-specificity requires further exploration. Two of the species recognised here, P. glebulae and P. munozae n. sp., showed apparent intra-individual variation in the ITS2 rDNA sequences as demonstrated by clear, replicated double peaks in the electropherograms. Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

  9. Four new species of Paradiscogaster Yamaguti, 1934 (Digenea: Faustulidae) from batfishes (Perciformes: Ephippidae) on the Great Barrier Reef, Australia.

    PubMed

    Diaz, Pablo E; Cutmore, Scott C; Cribb, Thomas H

    2017-03-01

    Examination of three species of batfishes (Teleostei: Epphippidae) from off Lizard and Heron Islands on the Great Barrier Reef led to the discovery of specimens of the trematode genus Paradiscogaster Yamaguti, 1934 (Digenea: Faustulidae). Morphological analysis demonstrated that the new specimens represented four morphotypes which we interpret to be new species: Paradiscogaster martini n. sp., P. vichovae n. sp. and P. brayi n. sp. from Platax orbicularis (Forsskål) and P. pinnatus (Linnaeus) off Lizard Island, and P. nitschkei n. sp. from P. teira (Forsskål) off Heron Island. Published material was re-examined and the specimens identified as P. chaetodontis okinawensis Yamaguti, 1971 from P. pinnatus from Okinawa, Japan, actually represent the new species P. brayi n. sp., demonstrating that some species of Paradiscogaster have wide geographical distributions. ITS2 rDNA data for the four morphotypes differ by 4-39 base pairs confirming the delineation of the four species proposed. A feature of this study is the recognition of Platax spp. as an important host group for Paradiscogaster, with the new species placing them as the second richest host group for these parasites after the Chaetodontidae.

  10. Four species of Pseudorhabdosynochus (Monogenea: Diplectanidae) from the camouflage grouper Epinephelus polyphekadion (Perciformes: Serranidae) off New Caledonia.

    PubMed

    Schoelinck, Charlotte; Justine, Jean-Lou

    2011-05-01

    Gill diplectanid monogeneans from the camouflage grouper Epinephelus polyphekadion (Bleeker) collected in the coral reef lagoon of New Caledonia, South Pacific, comprise four species. Very few monogeneans were found in most fish examined. Pseudorhabdosynochus dionysos n. sp. has a sclerotised vagina with a robust trumpet, a robust primary canal and two chambers of similar size; it is close to P. bacchus Sigura, Chauvet & Justine, 2007. P. viscosus n. sp. has a sclerotised vagina with a robust trumpet, long primary canal with an extremely thin wall and two small chambers, and a male quadriloculate organ with a characteristic thickening at the extremity of its cone. P. crassus n. sp., the most abundant species, has a sclerotised vagina with a thin-walled trumpet, thin-walled primary canal which is always coiled anteriorly and two small chambers. P. huitoe Justine, 2007, P. manifestus Justine & Sigura, 2007 and P. crassus have very similar sclerotised vaginae; however, species of this 'huitoe complex' can be distinguished by measurements of the haptoral hard parts. A few diplectanid specimens found in a single specimen of E. polyphekadion were attributed to P. huitoe, a species originally described from E. maculatus (Bloch) and also rarely found in E. cyanopodus Richardson in New Caledonia; specimens from these three fish species are morphologically indistinguishable.

  11. Prosorhynchus maternus sp. n. (Digenea: Bucephalidae) from the Malabar grouper Epinephelus malabaricus (Perciformes: Serranidae) off New Caledonia.

    PubMed

    Bray, Rodney A; Justine, Jean-Lou

    2006-09-01

    A new species, Prosorhynchus maternus sp. n., is described from the serranid fish Epinephelus malabaricus (Bloch et Schneider) in the waters off New Caledonia. It belongs to a group of Prosorhynchus species from serranids in which the uterus is restricted to the postovarian region. Its distinguishing features include the vitellarium relatively distant from the rhynchus, the cirrus-sac relatively distant from the posterior testis, the distinctly pre-equatorial mouth and several other somatic ratios. New records of Prosorhynchus longisaccatus Durio et Manter, 1968 from Epinephelus cyanopodus (Richardson) and Prosorhynchoides lamprelli Bott et Cribb, 2005 from Caranx papuensis Alleyne et Macleay off New Caledonia are also included.

  12. Monogeneans from Epinephelus chlorostigma (Val.) (Perciformes: Serranidae) off New Caledonia, with the description of three new species of diplectanids.

    PubMed

    Justine, Jean-Lou; Henry, Emilie

    2010-10-01

    Gill monogeneans from the brownspotted grouper Epinephelus chlorostigma (Val.) collected in deep water off the coral barrier reef of New Caledonia, South Pacific, comprise seven species. These include the ancyrocephalid Haliotrema sp., the capsalid Allobenedenia cf. epinepheli Yamaguti, 1968, and five diplectanids, namely Pseudorhabdosynochus epinepheli (Yamaguti, 1938), reported in a previous paper, P. cyanopodus Sigura & Justine, 2008 and P. podocyanus Sigura & Justine, 2008, two species originally described from E. cyanopodus Richardson, P. stigmosus n. sp., P. exoticoides n. sp. and Diplectanum femineum n. sp. P. stigmosus is characterised by a sclerotised vagina with a straight primary canal, large ovoid primary chamber and spherical secondary chamber. P. exoticoides is a highly aberrant species, with a thick-walled male quadriloculate organ and a discoid sclerotised vagina with an exceptional structure. Interestingly, P. exoticoides resembles P. exoticus Sigura & Justine, 2008, a species from E. cyanopodus, and P. stigmosus resembles P. cyanopodus and P. podocyanus, also both from E. cyanopodus, suggesting close relationships between the diplectanid faunae of these two fish species. D. femineum belongs to a group of diplectanids, provisionally classified as 'Diplectanum' Diesing, 1858, which all share a small funnel-shaped male copulatory organ. In contrast to other members of this group which have no sclerotised vagina, D. femineum has a sclerotised vagina with the same organisation as those of species of Pseudorhabdosynochus Yamaguti, 1958. This suggests that the species of 'Diplectanum' from groupers are closer to Pseudorhabdosynochus than suggested by the structure of the male organs.

  13. Pseudorhabdosynochus quadratus n. sp. (Monogenea: Diplectanidae) from the white-streaked grouper Epinephelus ongus (Bloch) (Perciformes: Serranidae) off New Caledonia.

    PubMed

    Schoelinck, Charlotte; Justine, Jean-Lou

    2011-05-01

    Pseudorhabdosynochus quadratus n. sp. is described from three specimens collected from the gills of a rarely examined fish, the white-streaked grouper Epinephelus ongus (Bloch), caught off Nouméa, New Caledonia. The description is based on Berlese slides and observations of live specimens; the soft parts are not described in detail. The species is characterised by a compact, sclerotised vagina with a robust trumpet, short primary canal, two closely associated chambers linked by a very short secondary canal and small accessory structure.

  14. Bucephalidae (Digenea) from epinephelines (Serranidae: Perciformes) from the waters off New Caledonia, including Neidhartia lochepintade n. sp.

    PubMed

    Bray, Rodney A; Justine, Jean-Lou

    2013-01-01

    Many bucephalid species, mainly of the subfamily Prosorhynchinae, have been described from epinepheline serranids (groupers) throughout the World's Oceans. In this paper eight named prosorhynchine species are described and/or illustrated from epinepheline fishes from New Caledonia. Neidhartia lochepintade n. sp. in Epinephelus chlorostigma differs from other Neidhartia spp. in various combinations of distinct body-size, rhynchus size, previtelline and pre-mouth distance, post-testicular distance, cirrus-sac reach and egg-size. Other species are: Neidhartia haywardi Bott, Miller & Cribb, 2013 in Plectropomus leopardus; Neidhartia tyleri Bott, Miller & Cribb, 2013 in Plectropomus leopardus and Plectropomus laevis; Prosorhynchus freitasi Nagaty, 1937 in Plectropomus leopardus and Plectropomus laevis; Prosorhynchus robertsthomsoni Bott & Cribb, 2009 in Cephalopholis argus; Prosorhynchus longisaccatus Durio & Manter, 1968 in Cephalopholis urodeta, Epinephelus areolatus, Epinephelus cyanopodus and Epinephelus maculatus. Prosorhynchus luzonicus Velasquez, 1959 and Prosorhynchus sp. B. in Epinephelus coioides; Prosorhynchus serrani Durio & Manter, 1968 in Variola albimarginata and Variola louti; Prosorhynchus sp. A in Epinephelus morrhua; Prosorhynchus sp. immature in Epinephelus coeruleopunctatus. The new combination Neidhartia longivesicula (Bilqees, Khalil, Khan, Perveen & Muti-ur-Rehman, 2009) (Syn. Prosorhynchus longivesicula) is formed. Evidence from this paper and earlier molecular studies indicates that there are numerous morphologically similar prosorhynchine species in serranids, most of which show a high degree of host-specificity. © R.A. Bray and J.-L. Justine, published by EDP Sciences, 2013.

  15. Feeding of introduced species of Cichla (Perciformes, Cichlidae) in Volta Grande reservoir, River Grande (MG/SP).

    PubMed

    Gomiero, L M; Braga, F M S

    2004-11-01

    Both species studied, Cichla cf. ocellaris and Cichla monoculus, fed mainly on fish, the first on Cichla spp., Plagioscion squamosissimus, and Tilapia rendalli, and the second, Cichla monoculus, on Cichla spp. Both diets indicated a strong correlation among the food items. Remarkable ontogenetic change was noted in both species diet: the young fed on crustaceans and insects while the adults fed mainly on fishes. The species studied, which are highly adapted to a brief life span, cause serious damage to the fish communities by predation, competition, and cascade effects throughout the whole trophic chain.

  16. Actinobdella inequinnulata (Annelida: Hirudinida:Rhynchobdellida:Glossiphoniidae) from White Crappie, Pomoxis annularis (Perciformes: Centrarchidae), in Arkansas, USA

    EPA Science Inventory

    One of 4 (25%) white crappie, Pomoxis annularis from the Ouachita River, Dallas County, Arkansas, was found to be infested with 8 glossiphoniid leeches, Actinobdella inequiannulata Moore, 1901. Leeches were removed from within the oeprculum on gills and gill arches. This leech i...

  17. Peripatric differentiation among adjacent marine lake and lagoon populations of a coastal fish, Sphaeramia orbicularis (Apogonidae, Perciformes, Teleostei).

    PubMed

    Gotoh, Ryo O; Sekimoto, Hidekatsu; Chiba, Satoru N; Hanzawa, Naoto

    2009-08-01

    The effect of geographical isolation on speciation, particularly within short geographical ranges, is poorly understood among marine organisms. Focusing on marine lakes of the Palau Islands, we investigated the effect of geographical isolation on Sphaeramia orbicularis, a coastal fish inhabiting marine lakes and lagoons. We collected a total of 157 individuals from three meromictic marine lakes and three lagoon sites, and analyzed the genetic diversity and differentiation of the populations based on complete sequences of the mitochondrial control region (824 bp). The analyses show that the genetic diversity of marine lake populations is much lower than that of lagoon populations. Moreover, a mismatch distribution analysis suggests that marine lake populations have experienced a decrease followed by a rapid expansion of their population size. These results reveal that marine lake populations have experienced severe founder and/or bottleneck events during the last thousand to tens of thousand years. Pairwise Phi(ST )values ranged from 0.531 to 0.848 between marine lake and lagoon populations and from 0.429 to 0.870 among marine lake populations, indicating a high degree of genetic differentiation. We speculate that such peripatric differentiation between marine lake and lagoon populations was caused by a small number of individuals colonizing the lakes from the lagoon (founder event) followed by repetitive bottleneck events, such as those generated by the El Niño-Southern Oscillation (ENSO). So far, such high genetic divergences in extremely short geographical ranges (approximately 150-250 m) have scarcely been reported for marine organisms. We suggest that the marine lake is one of the good model of geographical isolation in marine organisms and each marine lake population is in the early stages of speciation.

  18. Parasite communities of European perch, Perca fluviatilis L. (Actinopterygii: Perciformes: Percidae) from Lake Łebsko (Central Coast, Poland).

    PubMed

    Morozińska-Gogol, Jolanta

    2013-01-01

    Communities of parasites of European perch from lake Łebsko were studied and compared with similar communities from the Polish coastal zone. Parasites comprised 18 autogenic and 5 allogenic species. Most individual parasites belonged to allogenic species and were in larval stages. The majority of specimens were eye parasites with Tylodelphys clavata as the eudominant species. The dominant species, Acanthocephalus lucii, belongs to the intestine parasite community. Three marine species were found: Bothriocephalus scorpii, Hysterothylacium aduncum and Echinorchynhus gadi. The results indicate that the parasite fauna consists mostly of freshwater species, common in various types of European waters, while marine species were rarely observed.

  19. Gene rearrangements and evolution of tRNA pseudogenes in the mitochondrial genome of the parrotfish (Teleostei: Perciformes: Scaridae).

    PubMed

    Mabuchi, Kohji; Miya, Masaki; Satoh, Takashi P; Westneat, Mark W; Nishida, Mutsumi

    2004-09-01

    Genomic size of animal mitochondrial DNA is usually minimized over time. Thus, when regional duplications occur, they are followed by a rapid elimination of redundant material. In contrast to this general view, we report here long-sustained tRNA pseudogenes in the mitochondrial genome (mitogenome) of teleost fishes of the family Scaridae (parrotfishes). During the course of a molecular phylogenetic study of the suborder Labroidei, we determined the complete nucleotide sequence of the mitogenome for a parrotfish, Chlorurus sordidus, and found a gene rearrangement accompanied by a tRNA pseudogene. In the typical gene order of vertebrates, a tRNA-gene cluster between ND1 and ND2 genes includes tRNA(Ile) (I), tRNA(Gln) (Q), and tRNA(Met) (M) genes in this order (IQM). However, in the mitogenome of the parrotfish, the tRNA(Met) gene was inserted between the tRNA(Ile) and the tRNA(Gln) genes, and the tRNA(Gln) gene was followed by a putative tRNA(Met) pseudogene (psiM). Such a tRNA gene rearrangement including a pseudogene (IMQpsiM) was found in all of the 10 examined species, representing 7 of the 10 currently recognized scarid genera. All sister groups examined (20 species of Labridae and a single species of Odacidae) had the typical gene order of vertebrate mitogenomes. Phylogenetic analysis of the tRNA(Met) genes and the resulting pseudogenes demonstrated that the ancestral tRNA(Met) gene was duplicated in a common ancestor of the parrotfish. Based on the fossil record, these results indicate that the pseudogenes have survived at least 14 million years. Most of the vertebrate mitochondrial gene rearrangements involving the IQM region have held the tRNA(Met) gene just upstream of the ND2 gene, and even in a few exceptional cases, including the present ones, the tRNA pseudogenes have been found in that position. In addition, most of these tRNA(Met) pseudogenes maintained clover-leaf secondary structures, with the remainder sustaining the clover-leaf structure in the "top half (TpsiC and acceptor arms). Considering their potential secondary structures (holding "top halves" of the clover-leaf structures), locations within mitogenomes (flanking the 5' ends of the ND2 genes) and stabilities over time (survived at least 14 Myr), it is likely that the tRNA pseudogenes retain function as punctuation marks for mitochondrial ND2 mRNA processing.

  20. Niche partitioning of feeding microhabitats produces a unique function for herbivorous rabbitfishes (Perciformes, Siganidae) on coral reefs

    NASA Astrophysics Data System (ADS)

    Fox, R. J.; Bellwood, D. R.

    2013-03-01

    Niche theory predicts that coexisting species minimise competition by evolving morphological or behavioural specialisations that allow them to spread out along resource axes such as space, diet and temporal activity. These specialisations define how a species interacts with its environment and, by extension, determine its functional role. Here, we examine the feeding niche of three species of coral reef-dwelling rabbitfishes (Siganidae, Siganus). By comparing aspects of their feeding behaviour (bite location, bite rate, foraging distance) with that of representative species from two other abundant herbivorous fish families, the parrotfishes (Labridae, Scarus) and surgeonfishes (Acanthuridae, Acanthurus), we examine whether rabbitfishes have a feeding niche distinct from other members of the herbivore guild. Measurements of the penetration of the fishes' snouts and bodies into reef concavities when feeding revealed that rabbitfish fed to a greater degree from reef crevices and interstices than other herbivores. There was just a 40 % overlap in the penetration-depth niche between rabbitfish and surgeonfish and a 45 % overlap between rabbitfish and parrotfish, compared with the almost complete niche overlap (95 %) recorded for parrotfish and surgeonfish along this spatial niche axis. Aspects of the morphology of rabbitfish which may contribute to this niche segregation include a comparatively longer, narrower snout and narrower head. Our results suggest that sympatric coexistence of rabbitfish and other reef herbivores is facilitated by segregation along a spatial (and potentially dietary) axis. This segregation results in a unique functional role for rabbitfishes among roving herbivores that of "crevice-browser": a group that specifically feeds on crevice-dwelling algal or benthic organisms. This functional trait may have implications for reef ecosystem processes in terms of controlling the successional development of crevice-based algal communities, reducing their potential to trigger macroalgal outbreaks.

  1. Comparative Mitogenomics of the Genus Odontobutis (Perciformes: Gobioidei: Odontobutidae) Revealed Conserved Gene Rearrangement and High Sequence Variations

    PubMed Central

    Ma, Zhihong; Yang, Xuefen; Bercsenyi, Miklos; Wu, Junjie; Yu, Yongyao; Wei, Kaijian; Fan, Qixue; Yang, Ruibin

    2015-01-01

    To understand the molecular evolution of mitochondrial genomes (mitogenomes) in the genus Odontobutis, the mitogenome of Odontobutis yaluensis was sequenced and compared with those of another four Odontobutis species. Our results displayed similar mitogenome features among species in genome organization, base composition, codon usage, and gene rearrangement. The identical gene rearrangement of trnS-trnL-trnH tRNA cluster observed in mitogenomes of these five closely related freshwater sleepers suggests that this unique gene order is conserved within Odontobutis. Additionally, the present gene order and the positions of associated intergenic spacers of these Odontobutis mitogenomes indicate that this unusual gene rearrangement results from tandem duplication and random loss of large-scale gene regions. Moreover, these mitogenomes exhibit a high level of sequence variation, mainly due to the differences of corresponding intergenic sequences in gene rearrangement regions and the heterogeneity of tandem repeats in the control regions. Phylogenetic analyses support Odontobutis species with shared gene rearrangement forming a monophyletic group, and the interspecific phylogenetic relationships are associated with structural differences among their mitogenomes. The present study contributes to understanding the evolutionary patterns of Odontobutidae species. PMID:26492246

  2. A Comprehensive Description and Evolutionary Analysis of 22 Grouper (Perciformes, Epinephelidae) Mitochondrial Genomes with Emphasis on Two Novel Genome Organizations

    PubMed Central

    Zhang, Xiang; Ding, Shaoxiong

    2013-01-01

    Groupers of the family Epinephelidae are a diverse and economically valuable group of reef fishes. To investigate the evolution of their mitochondrial genomes we characterized and compared these genomes among 22 species, 17 newly sequenced. Among these fishes we identified three distinct genome organizations, two of them never previously reported in vertebrates. In 19 of these species, mitochondrial genomes followed the typical vertebrate canonical organization with 13 protein-coding genes, 22 tRNAs, two rRNAs, and a non-coding control region. Differing from this, members of genus Variola have an extra tRNA-Ile between tRNA-Val and 16S rRNA. Evidence suggests that this evolved from tRNA-Val via a duplication event due to slipped strand mispairing during replication. Additionally, Cephalopholisargus has an extra tRNA-Asp in the midst of the control region, likely resulting from long-range duplication of the canonical tRNA-Asp through illicit priming of mitochondrial replication by tRNAs. Along with their gene contents, we characterized the regulatory elements of these mitochondrial genomes’ control regions, including putative termination-associated sequences and conserved sequence blocks. Looking at the mitochondrial genomic constituents, rRNA and tRNA are the most conserved, followed by protein-coding genes, and non-coding regions are the most divergent. Divergence rates vary among the protein-coding genes, and the three cytochrome oxidase subunits (COI, II, III) are the most conserved, while NADH dehydrogenase subunit 6 (ND6) and the ATP synthase subunit 8 (ATP8) are the most divergent. We then tested the phylogenetic utility of this new mt genome data using 12 protein-coding genes of 48 species from the suborder Percoidei. From this, we provide further support for the elevation of the subfamily Epinephelinae to family Epinephelidae, the resurrection of the genus Hyporthodus, and the combination of the monotypic genera Anyperodon and Cromileptes to genus Epinephelus, and Aethaloperca to genus Cephalopholis. PMID:23951357

  3. Developmental and Microbiological Analysis of the Inception of Bioluminescent Symbiosis in the Marine Fish Nuchequula nuchalis (Perciformes: Leiognathidae)▿

    PubMed Central

    Dunlap, Paul V.; Davis, Kimberly M.; Tomiyama, Shinichi; Fujino, Misato; Fukui, Atsushi

    2008-01-01

    Many marine fish harbor luminous bacteria as bioluminescent symbionts. Despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of their symbioses. To gain insight on these issues, we examined wild-caught larvae of the leiognathid fish Nuchequula nuchalis, a species that harbors Photobacterium leiognathi as its symbiont, for the presence, developmental state, and microbiological status of the fish's internal, supraesophageal light organ. Nascent light organs were evident in the smallest specimens obtained, flexion larvae of 6.0 to 6.5 mm in notochord length (NL), a developmental stage at which the stomach had not yet differentiated and the nascent gasbladder had not established an interface with the light organ. Light organs of certain of the specimens in this size range apparently lacked bacteria, whereas light organs of other specimens of 6.5 mm in NL and of all larger specimens harbored large populations of bacteria, representatives of which were identified as P. leiognathi. Bacteria identified as Vibrio harveyi were also present in the light organ of one larval specimen. Light organ populations were composed typically of two or three genetically distinct strain types of P. leiognathi, similar to the situation in adult fish, and the same strain type was only rarely found in light organs of different larval, juvenile, or adult specimens. Light organs of larvae carried a smaller proportion of strains merodiploid for the lux-rib operon, 79 of 249 strains, than those of adults (75 of 91 strains). These results indicate that light organs of N. nuchalis flexion and postflexion larvae of 6.0 to 6.7 mm in NL are at an early stage of development and that inception of the symbiosis apparently occurs in flexion larvae of 6.0 to 6.5 mm in NL. Ontogeny of the light organ therefore apparently precedes acquisition of the symbiotic bacteria. Furthermore, bacterial populations in larval light organs near inception of the symbiosis are genetically diverse, like those of adult fish. PMID:18978090

  4. Pogonophryne neyelovi, a new species of Antarctic short-barbeled plunderfish (Perciformes, Notothenioidei, Artedidraconidae) from the deep Ross Sea

    PubMed Central

    Shandikov, Gennadiy A.; Eakin, Richard R.

    2013-01-01

    Abstract This paper continues descriptions of new deep-water Antarctic barbeled plunderfishes of the poorly known and the most speciose notothenioid genus Pogonophryne. It is based on a comprehensive collection obtained by the authors in 2009–2010 during an Antarctic toothfish (Dissostichus mawsoni) fishing trip. A new species, the hopbeard plunderfish Pogonophryne neyelovi, the twenty-second species of the genus, is described. The new species belongs to dorsally-spotted short-barbeled species forming the “Pogonophryne mentella” group. Pogonophryne neyelovi sp. n. is characterized by the following combination of characters: a very short and small mental barbel with an ovaloid and short terminal expansion covered by flattened scale-like processes that are mostly bluntly palmate; a moderately protruding lower jaw; a high second dorsal fin almost uniformly black and lacking a sharply elevated anterior lobe; pectoral fins striped anteriorly and uniformly light posteriorly; the anal and pelvic fins light; the dorsal surface of the head and the area anterior to the first dorsal fin covered with large, irregular dark brown blotches and spots; the ventral surface of the head, breast and belly without sharp dark markings. The new species is compared to the closest species Pogonophryne brevibarbata, Pogonophryne tronio, and Pogonophryne ventrimaculata. English vernacular names are proposed for all species of the genus. PMID:23794878

  5. Tosanoides obama, a new basslet (Perciformes, Percoidei, Serranidae) from deep coral reefs in the Northwestern Hawaiian Islands

    PubMed Central

    Pyle, Richard L.; Greene, Brian D.; Kosaki, Randall K.

    2016-01-01

    Abstract The new species Tosanoides obama is described from two specimens collected at a depth of 90–92 m off Kure Atoll and Pearl and Hermes Atoll, Northwestern Hawaiian Islands. It differs from the other two species of this genus in life color and in certain morphological characters, such as number of pored lateral-line scales, pectoral-fin rays, snout length, anterior three dorsal-fin spine lengths, dorsal-fin profile, and other characters. There are also substantial genetic differences from the other two species of Tosanoides (d ≈ 0.10 in mtDNA cytochrome oxidase I). The species is presently known only from the Northwestern Hawaiian Islands within the Papahānaumokuākea Marine National Monument. PMID:28138296

  6. Molecular systematics of the Jacks (Perciformes: Carangidae) based on mitochondrial cytochrome b sequences using parsimony, likelihood, and Bayesian approaches.

    PubMed

    Reed, David L; Carpenter, Kent E; deGravelle, Martin J

    2002-06-01

    The Carangidae represent a diverse family of marine fishes that include both ecologically and economically important species. Currently, there are four recognized tribes within the family, but phylogenetic relationships among them based on morphology are not resolved. In addition, the tribe Carangini contains species with a variety of body forms and no study has tried to interpret the evolution of this diversity. We used DNA sequences from the mitochondrial cytochrome b gene to reconstruct the phylogenetic history of 50 species from each of the four tribes of Carangidae and four carangoid outgroup taxa. We found support for the monophyly of three tribes within the Carangidae (Carangini, Naucratini, and Trachinotini); however, monophyly of the fourth tribe (Scomberoidini) remains questionable. A sister group relationship between the Carangini and the Naucratini is well supported. This clade is apparently sister to the Trachinotini plus Scomberoidini but there is uncertain support for this relationship. Additionally, we examined the evolution of body form within the tribe Carangini and determined that each of the predominant clades has a distinct evolutionary trend in body form. We tested three methods of phylogenetic inference, parsimony, maximum-likelihood, and Bayesian inference. Whereas the three analyses produced largely congruent hypotheses, they differed in several important relationships. Maximum-likelihood and Bayesian methods produced hypotheses with higher support values for deep branches. The Bayesian analysis was computationally much faster and yet produced phylogenetic hypotheses that were very similar to those of the maximum-likelihood analysis. (c) 2002 Elsevier Science (USA).

  7. Pseudascarophis brasiliensis sp. nov. (Nematoda: Cystidicolidae) parasitic in the Bermuda chub Kyphosus sectatrix (Perciformes: Kyphosidae) from southeastern Brazil

    PubMed Central

    Pereira, Felipe Bisaggio; Pereira, Aldenice de Nazaré; Timi, Juan Tomás; Luque, José Luis

    2013-01-01

    A new species of Pseudascarophis (Nematoda: Cystidicolidae) found in the stomach of Kyphosus sectatrix (Linnaeus) (Kyphosidae), off Rio de Janeiro, Brazil, is described. The new species can be differentiated from the other congeners by the presence of lateral alae, distinct but inconspicuous cephalic papillae at the anterior end, three pairs of precloacal and one pair of adcloacal papillae in males, egg morphology and morphometry of glandular oesophagus and spicules. Pseudascarophis tropica is transferred to Ascarophis as Ascarophis tropica (Solov'eva) comb. n. due to its ambiguous diagnosis. PMID:23828003

  8. Pseudascarophis brasiliensis sp. nov. (Nematoda: Cystidicolidae) parasitic in the Bermuda chub Kyphosus sectatrix (Perciformes: Kyphosidae) from southeastern Brazil.

    PubMed

    Pereira, Felipe Bisaggio; Pereira, Aldenice de Nazaré; Timi, Juan Tomás; Luque, José Luis

    2013-06-01

    A new species of Pseudascarophis (Nematoda: Cystidicolidae) found in the stomach of Kyphosus sectatrix (Linnaeus) (Kyphosidae), off Rio de Janeiro, Brazil, is described. The new species can be differentiated from the other congeners by the presence of lateral alae, distinct but inconspicuous cephalic papillae at the anterior end, three pairs of precloacal and one pair of adcloacal papillae in males, egg morphology and morphometry of glandular oesophagus and spicules. Pseudascarophis tropica is transferred to Ascarophis as Ascarophis tropica (Solov'eva) comb. n. due to its ambiguous diagnosis.

  9. The complete mitochondrial genome of bighead croaker, Collichthys niveatus (Perciformes, Sciaenidae): structure of control region and phylogenetic considerations.

    PubMed

    Xu, Tian-Jun; Cheng, Yuan-Zhi; Sun, Yue-Na; Shi, Ge; Wang, Ri-Xin

    2011-10-01

    Sciaenidae is a diverse, commercially important family. To understand the phylogenetic position of Collichthys niveatus in this family, we present its complete mitochondrial genome sequence. The genome is 16469 bp in length and contains 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes) and a control region (CR) as in other bony fishes. Further sequencing for the complete control region was performed on Collichthys lucida. Although the conserved sequence domains such as extend termination associated sequence (ETAS) and conserved sequence block domains (CSB-1, CSB-2 and CSB-3) are recognized in the control region of the two congeneric species, the typical central conserved blocks (CSB-F, CSB-E and CSB-D) could not be detected, while they are found in Miichthys miiuy and Cynoscion acoupa of Sciaenidae and other Percoidei fishes. Phylogenetic analyses do not support the monophyly of Pseudosciaeniae, which is against with the morphological results. C. niveatus is most closely related to Larimichthys polyactis, and Collichthys and Larimichthys may be merged into one genus, based on the current datasets.

  10. Genomic Organization of Repetitive DNA Elements and Its Implications for the Chromosomal Evolution of Channid Fishes (Actinopterygii, Perciformes)

    PubMed Central

    Cioffi, Marcelo de Bello; Bertollo, Luiz Antonio Carlos; Villa, Mateo Andres; de Oliveira, Ezequiel Aguiar; Tanomtong, Alongklod; Yano, Cassia Fernanda; Supiwong, Weerayuth; Chaveerach, Arunrat

    2015-01-01

    Channid fishes, commonly referred to as “snakeheads”, are currently very important in Asian fishery and aquaculture due to the substantial decline in natural populations because of overexploitation. A large degree of chromosomal variation has been found in this family, mainly through the use of conventional cytogenetic investigations. In this study, we analyzed the karyotype structure and the distribution of 7 repetitive DNA sequences in several Channa species from different Thailand river basins. The aim of this study was to investigate the chromosomal differentiation among species and populations to improve upon the knowledge of its biodiversity and evolutionary history. Rearrangements, such as pericentric inversions, fusions and polyploidization, appear to be important events during the karyotypic evolution of this genus, resulting in the chromosomal diversity observed among the distinct species and even among populations of the same species. In addition, such variability is also increased by the genomic dynamism of repetitive elements, particularly by the differential distribution and accumulation of rDNA sequences on chromosomes. This marked diversity is likely linked to the lifestyle of the snakehead fishes and their population fragmentation, as already identified for other fish species. The karyotypic features highlight the biodiversity of the channid fishes and justify a taxonomic revision of the genus Channa, as well as of the Channidae family as a whole, as some nominal species may actually constitute species complexes. PMID:26067030

  11. Complete mitochondrial genome sequence of bighead croaker Collichthys niveatus (Perciformes, Sciaenidae): a mitogenomic perspective on the phylogenetic relationships of Pseudosciaeniae.

    PubMed

    Cheng, Jiao; Ma, Guo-qiang; Song, Na; Gao, Tian-xiang

    2012-01-10

    The monophyly and phylogenetic relationships of Pseudosciaeniae have long been controversial. Here we describe the mitochondrial genome (mitogenome) sequence of Collichthys niveatus. It is a circular double-stranded DNA molecule of 16,450 base pairs (bp) in length with a standard set of 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs), 13 protein-coding genes as well as a non-coding control region. The mitogenome of C. niveatus shared common features with those of other bony fishes in terms of gene arrangement, base composition, and tRNA structures. The C. niveatus mitogenome exhibited pronounced strand-specific asymmetry in nucleotide composition, which was also reflected in the codon usage of genes oriented in opposite directions. Contrary to the typical structure of the control region, the central conserved blocks (CSB-D, -E, and -F) could not be detected in C. niveatus mitogenome. Phylogenetic analysis based on whole mitogenome sequences provided strong support for the monophyly of Pseudosciaeniae, and sister-group relationships of C. niveatus+Collichthys lucidus and Larimichthys crocea+Larimichthys polyactis, which was consistent with the traditional taxonomy. Unexpected divergence was found in two C. niveatus mitogenomes and several hypotheses were proposed to explain this observation including misidentification and introgressive hybridization between C. niveatus and L. polyactis, and polyphyletic origin of C. niveatus. We considered species misidentification to be the main hypothesis. However, additional data is essential to test these proposed hypotheses. Copyright © 2011 Elsevier B.V. All rights reserved.

  12. [Trophic range of tilapia Oreochromis aureus (Perciformes: Cichlidae) in Infiernillo dam, Michoacán-Guerrero, Mexico].

    PubMed

    Jiménez-Badillo, M L; Nepita-Villanueva, M R

    2000-01-01

    The trophic spectrum of tilapia Oreochromis aureus (Steindachner 1864), was determined by stomach content analysis in 153 organisms collected during 1993 in Michoacán-Guerrero, México. The feeding status of the fish at the time of observation was evaluated by the examination of fat surrounding the gut, gastric replection and the condition coefficient. The quantitative evaluation of the food items was carried out by the combination of the following analysis: Frequency of Occurrence, Volumetric Method, Volumetric Mean Index and Relative Importance Index. Diet consisted of: detritus and vascular plant residues as a primary food; unicellular algae as secondary food; and remains of insects and fish, graminean seeds, filamentous algae, cladocerans, ostracods, rotifers and copepods as occasional food. We detected a difference in food preferences between juveniles and adults and a variation in the consumption proportions of some food items during the rainy and dry seasons. Thus, we concluded that O. aureus is an omnivorous species with preference for detritus and vascular plant remain. Feeding seems to be determined by the abundance of food items found in the habitat and adults showed a trend to eat only detritus. Feeding does not seem to be a limiting factor in the development of the tilapia in Infiernillo dam.

  13. [Reproduction, distribution and abundance of the fish Pseudupeneus grandisquamis (Perciformes: Mullidae), in the Gulf of Tehuantepec, Mexico].

    PubMed

    Ramos-Santiago, Eduardo; Ramírez-Gutiérrrez, José Martín; Mendoza-Rodríguez, Ricardo; Tapia-García, Margarito

    2006-12-01

    As result of its biological and ecological strategies, Pseudupeneus grandisquamis is a dominant species in the demersal community of the Gulf of Tehuantepec, Mexico. Our main objective was to understand these strategies with respect to distribution, abundance and reproduction. We analyzed 5,175 individuals representing partial collections from five oceanographic expeditions between 1989 and 1990. It is a typical demersal marine species, with a wide distribution on the continental shelf. The highest abundance of P. grandisquamis occurs in March and November, around the 40 m isobath, facing the Superior-Inferior lake systems and Mar Muerto Lagoon. Reproduction occurred during all of the months studied, particularly from August to October, corresponding to the rainy season, when the salinity and temperature is lower. The presence of juveniles, principally in November and March, suggests a long period of recruitment; they are distributed mainly in the Superior-Inferior Lagoons, which serves as a nursery area where they remain until they are adults. The total female to male sex ratio was nearly 1:1 throughout the year. The maximum total length was 213 mm and the size at first maturity was 138 mm TL. The high abundance and reproduction occur when the gulf has a high level of ecological production, in accordance with the dynamics of the system, where the influence of coastal lagoons is important. Protection strategies for the area above the continental shelf of the Gulf of Tehuantepec are recommended for the estuary processes and for the reproduction and rearing of a large number of species, including P. grandisquamis.

  14. Overlapping patterns of morphometric and genetic differentiation in the Mediterranean goby Pomatoschistus tortonesei Miller, 1968 (Perciformes, Gobiidae) in Tunisian lagoons.

    PubMed

    Mejri, Randa; Lo Brutto, Sabrina; Hassine, Nesrine; Arculeo, Marco; Ben Hassine, Oum Kalthoum

    2012-08-01

    The genetic and morphological variations of Pomatoschistus tortonesei Miller, 1968 were studied in samples collected from three Tunisian lagoons. The morphological analysis included 18 morphometric measurements and was based on linear discriminant analysis (LDA), whereas the genetic analysis was based on the 16S-rRNA and COI mitochondrial genes. Both analyses differentiated the populations and demonstrated consistently a well-supported differentiation between the western Mediterranean samples (Bizerta and Tunis South lagoons) and the eastern Mediterranean sample (El Bibane lagoon). The observed differentiation could be explained in terms of the geographic isolation of the various populations and the influence of environmental factors, which differ greatly between the different sites. The molecular results revealed that the populations are characterised by unique haplotypes which are well defined in relation to limited gene flow and restricted dispersal abilities. Additionally, it seems that local selective pressures have modelled biometrical variation. Morphological results can reflect a differential habitat use revealed in the cephalic features and a different response to hydrodynamic constraints developed in dissimilar dorsal and pelvic fin lengths. Copyright © 2012 Elsevier GmbH. All rights reserved.

  15. In situ video observations of two manefishes (perciformes: Caristiidae) in the mesopelagic zone of the northern Gulf of Mexico

    USGS Publications Warehouse

    Benfield, M.C.; Caruso, John H.; Sulak, K.J.

    2009-01-01

    This paper describes direct video observations of two manefishes, likely Paracaristius sp., from the mesopelagic waters of the north-central Gulf of Mexico. One fish was observed with a remotely operated vehicle at a depth of 829 m by an industrial ROV as part of the SERPENT Project. The second was observed at 496 m from a manned submersible. Little is known about the behavior of manefishes because most records result from net-collected material. Our observation demonstrates that manefishes are capable of precise locomotory and posture control using extended, erect fins and that the pelvic fins of these fishes are extended in a parachute-like manner. Moreover, one of the specimens exhibited an unusual vertical, sinusoidal oscillation of its caudal fin. One of the observations took place in association with a physonect siphonophore. These observations may include the deepest published record for a manefish in the Gulf of Mexico.

  16. Evolutionary history of the Lake Tanganyika cichlid tribe Lamprologini (Teleostei: Perciformes) derived from mitochondrial and nuclear DNA data.

    PubMed

    Sturmbauer, Christian; Salzburger, Walter; Duftner, Nina; Schelly, Robert; Koblmüller, Stephan

    2010-10-01

    Lake Tanganyika comprises a cichlid species flock with substrate-breeding and mouthbrooding lineages. While sexual selection via mate choice on male mating color is thought to boost speciation rates in mouthbrooding cichlids, this is not the case in substrate-breeding lamprologines, which mostly form stable pairs and lack sexual dichromatism. We present a comprehensive reconstruction of the evolution of the cichlid tribe Lamprologini, based upon mtDNA sequences and multilocus nuclear DNA (AFLP) markers. Twelve mtDNA clades were identified, seven of which were corroborated by the AFLP tree. The radiation is likely to have started about 5.3 MYA, contemporarily with that of the mouthbrooding C-lineage, and probably triggered by the onset of deep-water conditions in Lake Tanganyika. Neither the Congo- nor the Malagarazi River species form the most ancestral branch. Several conflicts in the mtDNA phylogeny with taxonomic assignments based upon color, eco-morphology and behavior could be resolved and complemented by the AFLP analysis. Introgressive hybridization upon secondary contact seems to be the most likely cause for paraphyly of taxa due to mtDNA capture in species involving brood-care helpers, while accidental hybridization best explains the para- or polyphyly of several gastropod shell breeders. Taxonomic error or paraphyly due to the survival of ancestral lineages appear responsible for inconsistencies in the genera Lamprologus and Neolamprologus. Copyright 2010 Elsevier Inc. All rights reserved.

  17. Evolutionary history of the Lake Tanganyika cichlid tribe Lamprologini (Teleostei: Perciformes) derived from mitochondrial and nuclear DNA data

    PubMed Central

    Sturmbauer, Christian; Salzburger, Walter; Duftner, Nina; Schelly, Robert; Koblmüller, Stephan

    2010-01-01

    Lake Tanganyika comprises a cichlid species flock with substrate-breeding and mouthbrooding lineages. While sexual selection via mate choice on male mating color is thought to boost speciation rates in mouthbrooding cichlids, this is not the case in substrate-breeding lamprologines, which mostly form stable pairs and lack sexual dichromatism. We present a comprehensive reconstruction of the evolution of the cichlid tribe Lamprologini, based upon mtDNA sequences and multilocus nuclear DNA (AFLP) markers. Twelve mtDNA clades were identified, seven of which were corroborated by the AFLP tree. The radiation is likely to have started about 5.3 MYA, contemporarily with that of the mouthbrooding C-lineage, and probably triggered by the onset of deep-water conditions in Lake Tanganyika. Neither the Congo- nor the Malagarazi River species form the most ancestral branch. Several conflicts in the mtDNA phylogeny with taxonomic assignments based upon color, eco-morphology and behavior could be resolved and complemented by the AFLP analysis. Introgressive hybridization upon secondary contact seems to be the most likely cause for paraphyly of taxa due to mtDNA capture in species involving brood-care helpers, while accidental hybridization best explains the para- or polyphyly of several gastropod shell breeders. Taxonomic error or paraphyly due to the survival of ancestral lineages appear responsible for inconsistencies in the genera Lamprologus and Neolamprologus. PMID:20601006

  18. Low Genetic and Morphometric Intraspecific Divergence in Peripheral Copadichromis Populations (Perciformes: Cichlidae) in the Lake Malawi Basin

    PubMed Central

    Anseeuw, Dieter; Raeymaekers, Joost A. M.; Busselen, Paul; Verheyen, Erik; Snoeks, Jos

    2011-01-01

    Peripheral isolated populations may undergo rapid divergence from the main population due to various factors such as a bottleneck or a founder effect followed by genetic drift or local selection pressures. Recent populations of two economically important Copadichromis species in Lake Malombe, a satellite lake of Lake Malawi, were neither genetically nor morphometrically distinct from their source populations in the main lake. Evidence was found for a founder effect which had a different impact on the genetic composition of the two species. In addition, the increased fishing pressure in Lake Malombe may have led to a reduction of the body sizes of both species. PMID:21716858

  19. Population genetic structure of Cichla pleiozona (Perciformes: Cichlidae) in the Upper Madera basin (Bolivian Amazon): sex-biased dispersal?

    PubMed

    Carvajal-Vallejos, F M; Duponchelle, F; Ballivian, J P Torrico; Hubert, Nicolas; Rodríguez, J Nuñez; Berrebi, P; Cornejo, S Sirvas; Renno, J-F

    2010-12-01

    This study investigates the population structure of the Tucunaré (Cichla pleiozona) in the Bolivian Amazon (Upper Madera) by using nuclear (EPIC-PCR, 67 individuals) and mitochondrial (Control Region, 41 published and 76 new sequences) DNA analyses, in relation with ecological (water quality: muddy, clear and mix) and geographic factors. Our analyses of both markers showed the highest diversity in clear waters (Yata, Middle and Upper Iténez), and the existence of two populations in muddy waters (Sécure and Ichilo) and one in mix waters (Manuripi). On the other hand, mitochondrial analyses identified three populations in clear waters where nuclear analyses identified a panmictic population. The highest diversity observed in the Yata-Iténez system suggests that an aquatic refuge occurred during the past in this area. The possible explanations for the observed discrepancy between nuclear and mitochondrial markers are discussed, and a sex-biased dispersal seems to be the most plausible hypothesis in the light of the available information and field observations. Copyright © 2010 Elsevier Inc. All rights reserved.

  20. First cytogenetic report in Cichlasoma sanctifranciscense Kullander, 1983 (Perciformes, Cichlidae) from northeastern Brazil with inferences on chromosomal evolution of Cichlasomatini

    PubMed Central

    Argôlo, Leandro A.; Affonso, Paulo Roberto Antunes de Mello

    2015-01-01

    Abstract Even though genetic aspects of some cichlids have been widely studied over the last decades, little is known about the genomic structure of Cichlidae when compared to the large number of species in the family. In this paper, the first chromosomal data for Cichlasoma sanctifranciscense Kullander, 1983 are presented and discussed based on cytotaxonomic and karyoevolutionary inferences on Cichlasomatini. All individuals shared a diploid number of 2n=48 distributed as 10sm+28st+10a and Ag-NORs on short arms of a submetacentric pair. Heterochromatin was detected at pericentromeric regions of most chromosomes and at terminal sites of a few pairs. GC-rich regions were observed on short arms of two biarmed pairs, including the pair bearing Ag-NORs. Double-FISH with ribosomal probes revealed 18S rDNA clusters coincident with GC-rich regions in two biarmed pairs and 5S rDNA at interstitial location of an acrocentric pair. Cichlasoma sanctifranciscense shares some symplesiomorphic traits described in Cichlidae (2n=48 and pericentromeric C-bands) while other chromosomal features diverge from the common trend reported in Cichlasomatini, such as multiple 18S rDNA sites combined with high FN values. Finally, the present results are useful to support taxonomic identification once species-specific markers have been provided in Cichlasoma sanctifranciscense. PMID:26753082

  1. A new species of Pseudocrenilabrus (Perciformes: Cichlidae) from Lake Mweru in the Upper Congo River System.

    PubMed

    Katongo, Cyprian; Seehausen, Ole; Snoeks, Jos

    2017-02-26

    Pseudocrenilabrus pyrrhocaudalis sp. nov. is described from Lake Mweru in the upper Congo River drainage, on the border of the Democratic Republic of Congo and Zambia. This species, which appears to be endemic to the lake, lives in sympatry with P. philander. Pseudocrenilabrus pyrrhocaudalis sp. nov. is distinguished from P. philander in nuptial males by the presence of an orange colour on the ventral part of the body and the proximal parts of the anal and caudal fins, a broad band of bright white on the distal edge of anal and caudal fins, a uniform grey head and dorsum, and a subtruncate caudal fin. In addition, P. pyrrhocaudalis has a shorter snout, a narrower head, a smaller interorbital distance, a smaller pre-anal distance, a more slender caudal peduncle and fewer scales around the caudal peduncle in both sexes.

  2. Pempheris ufuagari sp. nov., a new species in the genus Pempheris (Perciformes, Pempheridae) from the oceanic islands of Japan.

    PubMed

    Koeda, Keita; Yoshino, Tetsuo; Tachihara, Katsunori

    2013-01-29

    Pempheris ufuagari sp. nov. is described based on 10 specimens, 143.9-196.8 mm in standard length, collected from Mi-nami Daito Island and Ogasawara Islands, which are oceanic islands of Japan. Pempheris ufuagari is characterized by a distinct black spot on the pectoral fin base, a bright yellow dorsal and caudal fin, and a blackish band on the outer margin of the anal fin. Pempheris oualensis also has a large body and a distinct black spot on the pectoral fin base, and forms mixed schools with P. ufuagari, but can be distinguished by the different fin coloration (dorsal and caudal fin brown or pale; no blackish band on anal fin margin), and the presence of a villiform tooth band extending outside the lips. Although P. otaitensis, which is found in French Polynesia and Samoa, has similar coloration as P. ufuagari, the latter has lower scale counts than those of the former species: pored lateral-line scales 62-71 (vs. 70-79); scale rows above lateral line 6 1/2-7 1/2 (vs. 8 1/2); predorsal scales 37-43 (vs. 44-48).

  3. Revising the distribution of a threatened goby, Apocryptodon punctatus (Perciformes, Oxudercidae), in Japan with the discovery of an isolated population

    PubMed Central

    Murase, Atsunobu; Inui, Ryutei; Miki, Ryohei; Miyazaki, Yusuke

    2017-01-01

    Abstract Five specimens of a threatened goby, Apocryptodon punctatus (21.2–40.1 mm in standard length), were collected at a mudflat site of Kushima City, Miyazaki Prefecture, Kyushu, southern Japan over two seasons, autumn (September 2015) and spring (April 2016). A review of distributional records of Apocryptodon punctatus revealed that this population represents the southernmost record of the species in Japanese waters, and is isolated ca. 200 km south-southwest from the nearest point of the main range of the species along the Pacific coast of Japan. Publicising this population will help conserve it and its vulnerable habitat. PMID:28228668

  4. The chromosomal complement of the artedidraconid fish Histiodraco velifer (Perciformes: Notothenioidei) from Terra Nova Bay, Ross Sea.

    PubMed

    Caputo, V; Splendiani, A; Nisi Cerioni, P; Olmo, E

    2003-01-01

    The karyotype of Histiodraco velifer from the Antartic Ocean was analyzed using various banding methods and in situ hybridization with a telomeric probe. A male and a female had a diploid set of 46 chromosomes (6 submetacentric + 40 acrocentric, FN = 52); the nucleolar organizer was CMA3-positive and was located on the short arm of a medium-sized submetacentric pair. All chromosomes stained uniformly with DAPI, whereas C-banding revealed heterochromatic blocks that were mostly located centromerically and telomerically and were resistant to ALUI digestion. The substantial identity of the karyotype of H. velifer with that of the other artedidraconids investigated so far suggests that chromosome changes must have played a less than significant role in the speciation among the lineages of this fish family endemic to Antarctica.

  5. Reproduction, food dynamics and exploitation level of Oreochromis niloticus (Perciformes: Cichlidae) from artisanal fisheries in Barra Bonita Reservoir, Brazil.

    PubMed

    Novaes, José Luís Costa; Carvalho, Edmir Daniel

    2012-06-01

    Nile tilapia (Oreochromis niloticus), which is exotic to South America, is the most common species caught in artisanal fisheries at the Barra Bonita Reservoir, Southeastern Brazil. This species is of great socioeconomic importance for the region and keeps active a population of about 500 fishers. In the present study we assess reproduction, food dynamics and level of exploitation of O. niloticus, caught by artisanal fisheries in the Barra Bonita Reservoir. Specimens were collected monthly, from July 2004-June 2005, and a total of 1 715 specimens were analyzed. Each specimen was examined to obtain biological and biometric data: standard length (cm), total weight (g), reproductive data (sex and stage of maturation), and stomach contents (empty, partly full, and full). We also estimated the sex ratio (by macroscopic observation of gonads), reproductive period (by ovarian development and seasonal average of gonadosomatic index in females), and feeding habits (by stomach contents). The possible relationship between abiotic factors and the reproductive period was statistically verified using Spearman's Rank Correlation. The FiSAT (ELEFAN I) package was used to assess growth parameters, mortality rates and to infer exploitation rate from standard length frequencies. The O. niloticus population had a sex ratio of 1.3:1 (M:F). Results indicated that ripe females were captured throughout the year, with a higher frequency during the winter-2004 (with a frequency of 59%, at a mean temperature of 20.5 degreeC), and in spring-2004 (with a frequency of 60.5% at a mean temperature of 21.18 degreeC). The GSI mean values obtained by season were: winter-2004: 1.71; spring-2004: 1.72; summer-2005: 0.80, and autumn-2005: 1.19. The Spearman correlation indicated positive values with respect to pH, dissolved oxygen, electric conductivity, transparency and chlorophyll a, and negative values with respect to temperature, accumulated rainfall and altimetric benchmark. The main food items were phytoplankton and periphytic algae, observed in 99.6% of the analyzed stomachs. The estimated growth and mortality parameters were: Linfinity=33.60cm, k=0.63/year, longevity= 4.76years, Z=2.81/ year, M=1.20/year and F=1.61/year. The weight-length relationship was Ln Wt=-2.8532+2.8835 Ln Lp. The estimated yield per recruit values were as follows: E=0.570, Emax=0.776, E0.1=0.604 and E0.5=0.349. These results indicate that a well established population of O. niloticus is present at Barra Bonita Reservoir; with an active reproduction throughout the year, more intense during winter and spring, and that O. niloticus is a phyto-planktophagus species. There were no indications that this species is being overfished, we therefore recommend that, due to its exotic condition, no restrictions need to be taken on its fishing activities.

  6. [Feeding habits of sphoeroides testudineus (Perciformes: Tetraodontidae) in the lagoon system of Ria Lagartos, Yucatán, Mexico].

    PubMed

    Chi-Espínola, Ariel Adriano; Vega-Cendejas, Maria Eugenia

    2013-06-01

    Sphoeroides testudineus is a dominant species in the coastal systems of Yucatán. Because of its wide distribution, occurrence and abundance performs an important functional role in coastal ecosystems. We assessed the trophic preferences and trophic-level variation in space and time for this species in Ria Lagartos lagoon, an hyperhaline ecosystem located Northwest of Yucatan Peninsula. The specimens were collected bimonthly during two annual periods (2004-2005 and 2007-2008) in 23 sites distributed along the system into four zones (marine, channel, Coloradas West and Coloradas East). Spatial and seasonal trophic variations were evaluated using canonical correspondence analysis (ACC). In a total of 382 individuals, 68 food resources included in 20 trophic groups were obtained. Higher relative importance index values (IIR) were obtained for bivalves, gastropods and macrophytes. Seasonal trophic variation showed that bivalves and gastropods were consumed along the year, while macrophytes were a preferential food during rains and windy seasons. Spatial variation indicates lower gastropods consumption at the inner zone of the system, and the opposite ocurred with bivalves. The consumption ofmacrophytes may be due to their greater abundance during rains and in the channel zone. The ACC showed that spatial trophic variation was due because of the abundance of the preferential preys (bivalves, gastropods), while seasonal differences by the secondary (amphipods, eggs, nematodes, brachyuran, detritus, nematodes). The results contribute to the biological knowledge of the species and highlight the importance and trophic function of S testudineus for the energy transfer from the benthos to higher trophic levels of the food chain.

  7. [Age and growth of the hybrid tilapia Oreochromis niloticus x Oreochromis aureus (Perciformes: Cichlidae) in the dam "Zimapan" Mexico].

    PubMed

    Gómez-Ponce, M A; Granados-Flores, K; Padilla, C; López-Hernández, M; Núñez-Nogueira, G

    2011-06-01

    Studies on age and growth in fishes are essential to establish models on population dynamics. The previous issues were determined in this study by scale growth analyses. Between September 2003 and May 2004, 382 organisms were captured using gill and atarraya nets. Eight samples were developed every month a long this study. Standard length in centimeters (L(e)), total weight in grams (P9t)), scale length in centimeters (L(esc)) and growth rings were determined. The size and weight ranged from 38 to 232 mm (L(e)) and 8.7 to 311.9 g (P(t)), respectively. The growth rings analyses reveal four age groups, being the second group the most representative with 34.3% of the total sampled. The scale ring analyses showed two growth rings, one develops between November-January and the other between April-May, in good correlation with low temperature and gonad development, respectively. The growth parameters were established as: L infinity = 281.1mm, W infinity = 877.1g, K = 0.33 and T(0) = 0.88 year(-1). The growth curves based on length and weight were described by using the von Bertalanffy model: L(e) = 28.11[1 -e (-0.33(t+0.88))] and P(t) = 877.17[1-e (-0.33(t+0.88))]3. Finally, it is concluded that commercial catches in this dam are between a year and year and half-old when exploited.

  8. Evaluation of cytochrome b mtDNA sequences in genetic diversity studies of Channa marulius (Channidae: Perciformes).

    PubMed

    Habib, Maria; Lakra, W S; Mohindra, Vindhya; Khare, Praveen; Barman, A S; Singh, Akanksha; Lal, Kuldeep K; Punia, Peyush; Khan, Asif A

    2011-02-01

    Channa marulius (Hamilton, 1822) is a commercially important freshwater fish and a potential candidate species for aquaculture. The present study evaluated partial Cytochrome b gene sequence of mtDNA for determining the genetic variation in wild populations of C. marulius. Genomic DNA extracted from C. marulius samples (n = 23) belonging to 3 distant rivers; Mahanadi, Teesta and Yamuna was analyzed. Sequencing of 307 bp Cytochrome b mtDNA fragment revealed the presence of 5 haplotypes with haplotype diversity value of 0.763 and nucleotide diversity value of 0.0128. Single population specific haplotype was observed in Mahanadi and Yamuna samples and 3 haplotypes in Teesta samples. The analysis of data demonstrated the suitability of partial Cytochrome b sequence in determining the genetic diversity in C. marulius population.

  9. Growth, mortality and reproduction of the blue tilapia Oreochromis aureus (Perciformes: Cichlidae) in the Aguamilpa Reservoir, Mexico.

    PubMed

    Peña Messina, Emilio; Tapia Varela, Raul; Velázquez Abunader, José Iván; Orbe Mendoza, Alma Araceli; Velazco Arce, Javier Marcial de Jesús Ruiz

    2010-12-01

    Tilapia production has increased in Aguamilpa Reservoir, in Nayarit, Mexico, in the last few years and represents a good economic activity for rural communities and the country. We determined growth parameters, mortality and reproductive aspects for 2413 specimens of blue tilapia Oreochromis aureus in this reservoir. Samples were taken monthly from July 2000 through June 2001, of which 1 371 were males and 1 042 were females. Standard length (SL) and total weight (TW) were measured in each organism. The SL/TW relationships through power models for sexes were determined. The growth parameters L infinity k, and t0 of the von Bertalanffy equation were estimated using frequency distribution of length through ELEFAN-I computer program. Finally the reproductive cycle and size of first maturity were established using morph chromatic maturity scale. The results suggested that the males and females had negative allometric growth (b < 3). Significant differences were found between SL/TW model for the sexes, suggesting separate models for males and females. Results indicate that there are no differences in growth rates between sexes; the proposed parameters were L infinity = 43.33 cm standard length, k = 0.36/year and t0 = -0.43 years. Natural and fishing mortality coefficients were 0.83/year and 1.10/year, respectively. The estimated exploitation rate (0.57/year) suggested that during the study period the fishery showed signs of overfishing. Blue tilapia reproduces year-round; the highest activity occurs from January through May and size of first maturity was 23 cm SL. We conclude that it is necessary to establish a minimum catch size in this reservoir based on the reproductive behavior of this species.

  10. A new species of philichthyid copepod (Crustacea: Cyclopoida) parasitic on Stellifer spp. (Perciformes: Sciaenidae) from southeastern Brazil.

    PubMed

    Pombo, Maíra; Turra, Alexander; Paschoal, Fabiano; Luque, José Luis

    2015-03-02

    A new species of copepod, Colobomatus stelliferi n. sp., belonging to the cyclopoid family Philichthyidae Vogt, 1877 is proposed based on female specimens collected from the mandibular canals of three species of sciaenid teleosts: Stellifer brasiliensis (Schultz) (type-host), S. rastrifer (Jordan) and S. stellifer (Bloch), collected in Caraguatatuba Bay, State of São Paulo, Brazil. The new species can be distinguished from its closest congeners by the absence of lateral processes in the genital somite, the presence of one cephalic process in the cephalosome and one pair of dorso-lateral processes on the fused pedigerous somites. This is the first species of Colobomatus Hesse, 1873 described as parasites of species of the teleost genus Stellifer.

  11. A new species of the genus Scolopsis Cuvier, 1830 (Perciformes: Nemipteridae) from southern India and Sri Lanka.

    PubMed

    Mishra, S S; Biswas, Sudeepta; Russell, Barry C; Satpathy, K K; Selvanayagam, M

    2013-01-31

    Scolopsis igcarensis, a new species of monocle bream (family Nemipteridae) from the coastal waters of southern India and Sri Lanka is described. The species is distinguished from other species of the genus Scolopsis by a combination of the following characters: scales on top of head reaching forward to between anterior nostril and snout tip; lower margin of eye below the line from snout tip to upper pectoral fin base; a bony ridge below eye; a white band from behind eye to level of end of dorsal fin base.

  12. Actinobdella inequinnulata (Annelida: Hirudinida:Rhynchobdellida:Glossiphoniidae) from White Crappie, Pomoxis annularis (Perciformes: Centrarchidae), in Arkansas, USA

    EPA Science Inventory

    One of 4 (25%) white crappie, Pomoxis annularis from the Ouachita River, Dallas County, Arkansas, was found to be infested with 8 glossiphoniid leeches, Actinobdella inequiannulata Moore, 1901. Leeches were removed from within the oeprculum on gills and gill arches. This leech i...

  13. Development of the follicle complex and oocyte staging in red drum, Sciaenops ocellatus Linnaeus, 1776 (Perciformes, Sciaenidae).

    PubMed

    Grier, Harry J

    2012-08-01

    Pelagic egg development in red drum, Sciaenops ocellatus, is described using tiered staging. Based on mitosis and meiosis, there are five periods: Mitosis of Oogonia, Active Meiosis I, Arrested Meiosis I, Active Meiosis II, and Arrested Meiosis II. The Periods are divided into six stages: Mitotic Division of Oogonia, Chromatin Nucleolus, Primary Growth, Secondary Growth, Oocyte Maturation and Ovulation. The Chromatin Nucleolus Stage is divided into four steps: Leptotene, Zygotene, Pachytene, and Early Diplotene. Oocytes in the last step possess one nucleolus, dispersed chromatin with forming lampbrush chromosomes and lack basophilic ooplasm. The Primary Growth Stage, characterized by basophilic ooplasm and absence of yolk in oocytes, is divided into five steps: One-Nucleolus, Multiple Nucleoli, Perinucleolar, Oil Droplets, and Cortical Alveolar. During primary growth, the Balbiani body develops from nuage, enlarges and disperses throughout the ooplasm as both endoplasmic reticulum and Golgi develop within it. Secondary growth or vitellogenesis has three steps: Early Secondary Growth, Late Secondary Growth and Full-Grown. The Oocyte Maturation Stage, including ooplasmic and germinal vesicle maturation, has four steps: Eccentric Germinal Vesicle, Germinal Vesicle Migration, Germinal Vesicle Breakdown and Resumption of Meiosis when complete yolk hydration occurs. The period is Arrested Meiosis II. When folliculogenesis is completed, the ovarian follicle, an oocyte and encompassing follicle cells, is surrounded by a basement membrane and developing theca, all forming a follicle complex. After ovulation, a newly defined postovulatory follicle complex remains attached to the germinal epithelium. It is composed of a basement membrane that separates the postovulatory follicle from the postovulatory theca. Arrested Meiosis I encompasses primary and secondary growth (vitellogenesis) and includes most of oocyte maturation until the resumption of meiosis (Active Meiosis II). The last stage, Ovulation, is the emergence of the oocyte from the follicle when it becomes an egg or ovum.

  14. The novel finding of four distinct prepro-IGF-I E domains in a perciform fish, Sciaenops ocellatus, during ontogeny.

    PubMed

    Faulk, Cynthia K; Pérez-Domínguez, Rafael; Webb, Kenneth A; Holt, G Joan

    2010-10-01

    In fishes, insulin-like growth factor-I (IGF-I) stimulates growth and differentiation but also plays a role in a number of other processes including osmoregulation, metabolism, immune response and reproduction. This study presents the cDNA encoding multiple prepro-IGF-I transcripts obtained from red drum, Sciaenopsocellatus, and examines differential expression in select adult tissues and during ontogeny. Four distinct transcripts were sequenced which were identical in the coding region for the signal (132 bp) and mature (204 bp) peptides but differed in the coding region of the E peptide by the exclusion of 117 (Ea-1), 81 (Ea-2) or 36 (Ea-3) bp compared to the 222 bp present in Ea-4. Analysis of the pertinent portion of the genomic sequence of this gene suggests that the transcripts are a result of alternative splicing. This is the first report of the expression of all four known prepro-IGF-I transcripts in a teleost other than a salmonid. The deduced amino acid sequences exhibited 70-95% identity with teleosts and somewhat lower identity to other vertebrates (60-75%). Three of the 4 transcripts (Ea-2, Ea-3, Ea-4) were expressed in the liver, ovary, spleen, gall bladder, brain, red muscle, pancreas and spinal cord of adults. Only the Ea-4 transcript was expressed in adult stomach tissue while no signal was detected in pituitary, retina, intestine, adipose or white muscle. In contrast, all 4 transcripts were expressed throughout ontogeny. The apparent expression of the Ea-1 transcript only during the larval stage may indicate a developmental role for this E peptide in red drum.

  15. Pauciconfibula subsolana n. sp. (Monogenea: Microcotylidae) from Morone americana (Perciformes: Percichthyidae) collected in fresh water in New Brunswick, Canada.

    PubMed

    Chisholm, L A; Beverley-Burton, M; McAlpine, D F

    1991-12-01

    Pauciconfibula subsolana n. sp. (Monogenea: Microcotylidae) is proposed for parasites found on the inner surface of the operculum of Morone americana collected in the freshwater reaches of the Saint John River, near Mactaquac, New Brunswick. Pauciconfibula subsolana is differentiated from other species in the genus by the 2 posterolateral clamp sclerites, each of which is composed of 2 distinct sections, and by the absence of appendages on the eggs. An amended generic diagnosis is provided for Pauciconfibula. Pseudoaspinatrium Mamaev, 1986, is considered a junior synonym of Pauciconfibula Dillon and Hargis, 1965, and the species previously assigned to Pseudoaspinatrium are transferred to Pauciconfibula as Pauciconfibula euzeti (Ktari, 1971) n. comb., Pauciconfibula gallieni (Euzet and Ktari, 1971) n. comb., and Pauciconfibula pogoniae (MacCallum, 1913) n. comb.

  16. Complete mitochondrial genome and the phylogenetic position of the brown-spotted grouper Epinephelus chlorostigma (Perciformes: Epinephelidae).

    PubMed

    Chen, Xiao; Chen, Hao; Yang, Weidi; Lin, Lingling; Liu, Min

    2016-11-01

    The complete mitogenome of the brown-spotted grouper Epinephelus chlorostigma was determined in this study. It is 16,894 bp in length, containing 37 genes with the typical order and transcriptional direction in the vertebrates. The overall nucleotide composition is: 28.7% A; 28.0% C; 16.3% G and 27.0% T. The lengths of 22 transfer RNA (tRNA) genes range from 67 (tRNA-Cys) to 76 (tRNA-Leu1) bp. Two start codons (GTG and ATG) and two stop codons (TAG and TAA/T) were found in the protein-coding genes. The nodes of phylogenetic tree were strongly supported based on the complete mitogenomes of available groupers. The phylogenetic results suggested E. chlorostigma is the closest to E. areolatus within the genus Epinephelus.

  17. Haliotrematoides spp. (Monogenoidea: Dactylogyridae) infecting the gills of grunts (Perciformes: Haemulidae) from the southern coast of Quintana Roo, Mexico.

    PubMed

    Mendoza-Franco, Edgar F; Reyes-Lizama, C; González-Solís, D

    2009-12-01

    One previously described species, Haliotrema striatohamus Zhukov, 1981, found off the coast of Cuba and Campeche (Gulf of Mexico), is redescribed and transferred to Haliotrematoides Kritsky, Yang and Sun, 2009, as Haliotrematoides striatohamus ( Zhukov, 1981) n. comb. This transfer is based on the study of new specimens of this species found in the gill lamellae of grunts, i.e., Haemulon aurolineatum Cuvier, Haemulon plumierii (Lacepède), and Haemulon sciurus (Shaw) (Haemulidae), all from the southern coast of Quintana Roo, Mexico. In addition, another previously described species, Haliotrema brevispirocirrus Zhukov, 1981 from Haemulon striatum (Linnaeus) from Havana, Cuba, is also transferred to Haliotrematoides based on the comparative morphology of its original description. Data are provided for prevalence and mean intensity as well as for the morphologic and biometric variability of H. striatohamus from different hosts. The new replacement of H. striatohamus ( Zhukov, 1981) n. comb. and H. brevispirocirrus (Zhukov, 1981) n. comb. infecting these taxa represents the first known species of Haliotrematoides on these fishes and new geographical records for these dactylogyrid monogenoids.

  18. Digestive tract morphology of the Neotropical piscivorous fish Cichla kelberi (Perciformes: Cichlidae) introduced into an oligotrophic Brazilian reservoir.

    PubMed

    dos Santos, Alejandra Filippo Gonzalez Neves; dos Santos, Luciano Neves; Araújo, Francisco Gerson

    2011-09-01

    Despite being one of the most well-known cichlid fish of importance to artisanal and sport fishing, and among the largest fishes in the Neotropics, data on digestive tract anatomy of peacock basses (Cichla spp.) are largely lacking, especially for non-native populations. In this paper, we describe for the first time the digestive tract morphology of Cichla kelberi, a voracious piscivore that was introduced in the 1950s into an oligotrophic and physically low-complex impoundment in Brazil. Peacock basses were collected between 1994 and 2002 in Lajes Reservoir, through gillnets (25 to 55mm mesh; 20-50 x 2m), seines (10 x 2.5m; 8.0mm mesh), cast nets and angling. All the fishes were kept on ice in the field and then transferred to the laboratory, where they were identified, measured, weighed and dissected for digestive tract analyses. The Index of Relative Importance-IRI was calculated for diet characterization while linear and non-linear regressions were performed to assess growth patterns of four morphological characters related to feeding (e.g. mouth width, mouth height, stomach length and intestine length) and the number of gill rakers during the C. kelberi ontogeny. Most digestive tract structures were directly related to the piscivorous diet of C. kelberi, indicating that peacock bass is a diurnal, bathypelagic and gape-size limited predator that feeds largely on shallow-water prey species within the littoral zone. Mouth width and height grew allometrically (b > 1) with the size of peacock bass, broadening the size range in which prey can be eaten, but especially for predators smaller than -400mm of total length. Differently, stomach and intestine lengths increased isometrically (b = 1), which could constrain prey consumption for adult C. kelberi, especially those at advanced stages of gonadal maturation. The presence of longer-drawn, sharp and furcated gill rakers in C. kelberi may be related to increased prey retention in the resource-limited Lajes Reservoir, but further studies are necessary whether such features are randomly triggered by genetic or phenotypic anomalies, or effectively bring ecological advantages to the predator. In addition to contribute to improve the current biological knowledge on peacock basses, our results can be also useful to further comparisons on whether those morphological features related to feeding will change with transitions on prey consumption by C. kelberi and/or with the particular conditions of the invaded ecosystem.

  19. Parasite community of Cichla kelberi (Perciformes, Cichlidae) in the Três Marias Reservoir, Minas Gerais, Brazil.

    PubMed

    Santos-Clapp, Michelle Daniele; Brasil-Sato, Marilia Carvalho

    2014-01-01

    A total of 112 specimens of yellow peacock bass, Cichla kelberi, were collected in the Três Marias Reservoir, upper São Francisco River, State of Minas Gerais, between 2004 and 2005. Twelve species of parasites were collected: two species of ectoparasites (the monogenetic Gussevia sp. and the copepod Ergasilus sp.); and ten species of endoparasites, including four species of adult endoparasites: the cestodes Proteocephalus macrophallus and P. microscopicus, and the nematodes: Capillostrongyloides sentinosa and Rhabdochona acuminata, and six species of larval endoparasites: the metacercariae of Austrodiplostomum compactum and the nematodes: Hysterothylacium sp., Contracaecum sp. Type 1, Procamallanus (Spirocamallanus) inopinatus, Spiroxys sp., and Goezia sp.. A significant positive correlation in the abundance of the two adult endoparasites species, P. macrophallus and P. microscopicus, may indicate that such associated species had the same ecological requirements. The positive correlations between the prevalence of the larval endoparasites, Hysterothylacium sp. and A. compactum, may indicate that there was opportunism for the two generalists species. Ergasilus sp., C. sentinosa, R. acuminata and Hysterothylacium sp. were recorded for the first time in C. kelberi, and this increased their list of known hosts. In addition, both proteocephalid species and C. sentinosa can extend their known biogeographical distribution to the Três Marias Reservoir.

  20. Tosanoides obama, a new basslet (Perciformes, Percoidei, Serranidae) from deep coral reefs in the Northwestern Hawaiian Islands.

    PubMed

    Pyle, Richard L; Greene, Brian D; Kosaki, Randall K

    2016-01-01

    The new species Tosanoides obama is described from two specimens collected at a depth of 90-92 m off Kure Atoll and Pearl and Hermes Atoll, Northwestern Hawaiian Islands. It differs from the other two species of this genus in life color and in certain morphological characters, such as number of pored lateral-line scales, pectoral-fin rays, snout length, anterior three dorsal-fin spine lengths, dorsal-fin profile, and other characters. There are also substantial genetic differences from the other two species of Tosanoides (d ≈ 0.10 in mtDNA cytochrome oxidase I). The species is presently known only from the Northwestern Hawaiian Islands within the Papahānaumokuākea Marine National Monument.

  1. Repetitive DNAs highlight the role of chromosomal fusions in the karyotype evolution of Dascyllus species (Pomacentridae, Perciformes).

    PubMed

    Getlekha, Nuntaporn; Molina, Wagner Franco; de Bello Cioffi, Marcelo; Yano, Cassia Fernanda; Maneechot, Nuntiya; Bertollo, Luiz Antonio Carlos; Supiwong, Weerayuth; Tanomtong, Alongklod

    2016-04-01

    The Dascyllus genus consists of 11 species spread over vast regions of the Indo-Pacific, showing remarkable reductions in the diploid chromosome numbers (2n). The present study analyzed the karyotypes and other chromosomal characteristics of D. trimaculatus (2n = 48; 2st + 46a; NF = 50), D. carneus (2n = 48; 2st + 46a; NF = 50) and D. aruanus (2n = 30; 18m + 2st + 10a; NF = 50) from the Thailand Gulf (Pacific Ocean) and D. melanurus (2n = 48; 2st + 46a; NF = 50) from the Andaman Sea (Indian Ocean), employing conventional cytogenetic analyses and the chromosomal mapping of repetitive DNAs, using 18S and 5S rDNA, telomeric sequences and (CA)15, (GA)15, and (CAA)10 microsatellites as probes. The C-positive heterochromatin was found in the centromeric regions of most chromosomal pairs and 18S rDNA phenotypes were single in all species. However, in D. aruanus (2n = 30), which harbors nine metacentric pairs; the 5S rDNA sites were located in the centromeric region of the shortest one. The mapping of the telomeric sequences in D. aruanus revealed the presence of interstitial telomeric sites (ITS) in the centromeric region of four metacentric pairs, with one of these pairs also displaying an additional ITS in the long arms. Distinct chromosomal markers confirmed the reduction of the 2n by chromosomal fusions, highlighting the precise characterization of these rearrangements by the cytogenetic mapping of the repetitive DNAs.

  2. Genome Sequencing of the Perciform Fish Larimichthys crocea Provides Insights into Molecular and Genetic Mechanisms of Stress Adaptation

    PubMed Central

    Shi, Qiong; Zhu, Lv-Yun; Li, Ting; Ding, Yang; Nie, Li; Li, Qiuhua; Dong, Wei-ren; Jiang, Liang; Sun, Bing; Zhang, XinHui; Li, Mingyu; Zhang, Hai-Qi; Xie, ShangBo; Zhu, YaBing; Jiang, XuanTing; Wang, Xianhui; Mu, Pengfei; Chen, Wei; Yue, Zhen; Wang, Zhuo; Wang, Jun; Shao, Jian-Zhong; Chen, Xinhua

    2015-01-01

    The large yellow croaker Larimichthys crocea (L. crocea) is one of the most economically important marine fish in China and East Asian countries. It also exhibits peculiar behavioral and physiological characteristics, especially sensitive to various environmental stresses, such as hypoxia and air exposure. These traits may render L. crocea a good model for investigating the response mechanisms to environmental stress. To understand the molecular and genetic mechanisms underlying the adaptation and response of L. crocea to environmental stress, we sequenced and assembled the genome of L. crocea using a bacterial artificial chromosome and whole-genome shotgun hierarchical strategy. The final genome assembly was 679 Mb, with a contig N50 of 63.11 kb and a scaffold N50 of 1.03 Mb, containing 25,401 protein-coding genes. Gene families underlying adaptive behaviours, such as vision-related crystallins, olfactory receptors, and auditory sense-related genes, were significantly expanded in the genome of L. crocea relative to those of other vertebrates. Transcriptome analyses of the hypoxia-exposed L. crocea brain revealed new aspects of neuro-endocrine-immune/metabolism regulatory networks that may help the fish to avoid cerebral inflammatory injury and maintain energy balance under hypoxia. Proteomics data demonstrate that skin mucus of the air-exposed L. crocea had a complex composition, with an unexpectedly high number of proteins (3,209), suggesting its multiple protective mechanisms involved in antioxidant functions, oxygen transport, immune defence, and osmotic and ionic regulation. Our results reveal the molecular and genetic basis of fish adaptation and response to hypoxia and air exposure. The data generated by this study will provide valuable resources for the genetic improvement of stress resistance and yield potential in L. crocea. PMID:25835551

  3. Redescription of Cheilinus quinquecinctus Rüppell, 1835 (Pisces: Perciformes, Labridae), a valid endemic Red Sea wrasse.

    PubMed

    Bogorodsky, Sergey V; Alpermann, Tilman J; Mal, Ahmad O

    2016-08-31

    The labrid fish Cheilinus quinquecinctus Rüppell, originally described from the Red Sea, has long been regarded as a junior synonym of C. fasciatus (Bloch). Herein, both nominal species are redescribed, based on examination of the types and additional material from the Red Sea (for C. quinquecinctus) and the Indo-West Pacific (for C. fasciatus). Rüppell's description of Cheilinus quinquecinctus was originally based on three syntypes, and the most representative adult specimen is designated as the lectotype. We show that Cheilinus quinquecinctus is restricted to the Red Sea and the Gulf of Aden, and it differs from the similar C. fasciatus in having modally fewer gill rakers on the first gill arch, a total of 13-16 (mean 13.9, usually 13 or 14 ) (vs. 13-16, mean 14.7, usually 14 or 15), in developing a ragged posterior margin of the caudal fin with age (versus only upper and lower caudal-fin lobes developing with age), and in its color pattern. The phylogenetic analysis of the COI barcoding region accords with the species status of C. quinquecinctus with the placement of the two sister species in two divergent and reciprocally monophyletic evolutionary lineages. A full description of C. quinquecinctus and diagnosis of C. fasciatus is provided here for comparison. In addition, the data include a table of the results of the meristic and morphological examination of type and additional specimens of both species from throughout their distribution ranges as well as a table of gill-raker counts of all examined specimens. Underwater color photographs are provided for comparison of juveniles, females and males of both species.

  4. PERMANENT GENETIC RESOURCES: Isolation and characterization of nine microsatellite loci from the chub mackerel, Scomber japonicus (Perciformes, Scombridae).

    PubMed

    Yagishita, N; Kobayashi, T

    2008-03-01

    The stock abundance of the chub mackerel Scomber japonicus - a very important species for fisheries, particularly in Japan - in the Pacific Ocean off Japan has remained at a low level. For studying the population genetics of the chub mackerel, we isolated nine polymorphic microsatellite loci (12-31 alleles/locus; expected heterozygosity, 0.762-0.983) from this species. Cross-species amplification indicated that eight of the nine microsatellite loci in the blue mackerel S. australasicus were polymorphic and functional.

  5. The complete mitochondrial DNA sequence of the short mackerel (Rastrelliger brachysoma), and its phylogenetic position within Scombroidei, Perciformes.

    PubMed

    Jondeung, Amnuay; Karinthanyakit, Wirangrong

    2010-04-01

    In order to support studies of short mackerel population genetic structure in the Gulf of Thailand and phylogenetic relationships, the mitochondrial genome of the short mackerel, Rastrelliger brachysoma, has recently been determined by a partial cloning technique, long PCR with three pairs of newly designed primers and primer walking sequencing. The complete mitochondrial genome is 16,539 bp in length and contains 37 mitochondrial genes (13 protein-coding genes, 2 ribosomal RNA genes and 22 transfer RNA genes) and a control region (CR) as in other bony fishes. Within the 845-bp CR, we identified several conserved motifs. The phylogeny obtained by Bayesian analyses based on two nucleotide datasets corresponding to the cytb and nd2 mitochondrial genes strongly support the inclusion of R. brachysoma within the monophyletic tribe of Scombrini in the family Scombridae. The obtained phylogeny also reveals high-statistical support for the existence of two distinct groups indicating that Scombroidei and Xiphioidei are two separate suborders.

  6. Bucephalidae (Digenea) from epinephelines (Serranidae: Perciformes) from the waters off New Caledonia, including Neidhartia lochepintade n. sp.

    PubMed Central

    2013-01-01

    Many bucephalid species, mainly of the subfamily Prosorhynchinae, have been described from epinepheline serranids (groupers) throughout the World’s Oceans. In this paper eight named prosorhynchine species are described and/or illustrated from epinepheline fishes from New Caledonia. Neidhartia lochepintade n. sp. in Epinephelus chlorostigma differs from other Neidhartia spp. in various combinations of distinct body-size, rhynchus size, previtelline and pre-mouth distance, post-testicular distance, cirrus-sac reach and egg-size. Other species are: Neidhartia haywardi Bott, Miller & Cribb, 2013 in Plectropomus leopardus; Neidhartia tyleri Bott, Miller & Cribb, 2013 in Plectropomus leopardus and Plectropomus laevis; Prosorhynchus freitasi Nagaty, 1937 in Plectropomus leopardus and Plectropomus laevis; Prosorhynchus robertsthomsoni Bott & Cribb, 2009 in Cephalopholis argus; Prosorhynchus longisaccatus Durio & Manter, 1968 in Cephalopholis urodeta, Epinephelus areolatus, Epinephelus cyanopodus and Epinephelus maculatus. Prosorhynchus luzonicus Velasquez, 1959 and Prosorhynchus sp. B. in Epinephelus coioides; Prosorhynchus serrani Durio & Manter, 1968 in Variola albimarginata and Variola louti; Prosorhynchus sp. A in Epinephelus morrhua; Prosorhynchus sp. immature in Epinephelus coeruleopunctatus. The new combination Neidhartia longivesicula (Bilqees, Khalil, Khan, Perveen & Muti-ur-Rehman, 2009) (Syn. Prosorhynchus longivesicula) is formed. Evidence from this paper and earlier molecular studies indicates that there are numerous morphologically similar prosorhynchine species in serranids, most of which show a high degree of host-specificity. PMID:24351242

  7. [Distribution and abundance of Katsuwonus pelamis larvae (Perciformes: Scombridae) in the Gulf of Mexico, 1982-1992].

    PubMed

    Guevara-Rascado, Ma de Lourdes; Cerecedo-Escudero, José Luis; Sánchez-Regalado, Ramón

    2008-09-01

    The epipelagic fish known as skipjack (Katsuwonus pelamis) is considered inside the "little tuna" group. The species is distributed in tropical and subtropical waters worldwide. Despite its fisheries importance in Mexico, there is little knowledge about the species in the region. The information from 18 oceanographic cruises inside the Mexican exclusive economic zone in the Gulf of Mexico and Caribbean Sea, was analyzed for the period 1982 to 1992. Plankton was collected in a paired 60 cm mouth bongo net sampler with 0.333 mm mesh nets in oblique tows. The maximum abundance was in spring and summer; 1986 had the largest population (4 881.8544 X 10(9) larvae) and 1983 the lowest density (566.3748 X 10(9)). The skipjack has suitable conditions for reproduction in this area, and the southwestern region is the most productive. The spawning was greater in the summer. The annual biomass was estimated in 2 513 to 21 659 tons, the high value corresponded to 1986, with potential yields between 7 472 and 10 071 tons. A local fishery seems viable but needs further evidence.

  8. Developmental and microbiological analysis of the inception of bioluminescent symbiosis in the marine fish Nuchequula nuchalis (Perciformes: Leiognathidae).

    PubMed

    Dunlap, Paul V; Davis, Kimberly M; Tomiyama, Shinichi; Fujino, Misato; Fukui, Atsushi

    2008-12-01

    Many marine fish harbor luminous bacteria as bioluminescent symbionts. Despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of their symbioses. To gain insight on these issues, we examined wild-caught larvae of the leiognathid fish Nuchequula nuchalis, a species that harbors Photobacterium leiognathi as its symbiont, for the presence, developmental state, and microbiological status of the fish's internal, supraesophageal light organ. Nascent light organs were evident in the smallest specimens obtained, flexion larvae of 6.0 to 6.5 mm in notochord length (NL), a developmental stage at which the stomach had not yet differentiated and the nascent gasbladder had not established an interface with the light organ. Light organs of certain of the specimens in this size range apparently lacked bacteria, whereas light organs of other specimens of 6.5 mm in NL and of all larger specimens harbored large populations of bacteria, representatives of which were identified as P. leiognathi. Bacteria identified as Vibrio harveyi were also present in the light organ of one larval specimen. Light organ populations were composed typically of two or three genetically distinct strain types of P. leiognathi, similar to the situation in adult fish, and the same strain type was only rarely found in light organs of different larval, juvenile, or adult specimens. Light organs of larvae carried a smaller proportion of strains merodiploid for the lux-rib operon, 79 of 249 strains, than those of adults (75 of 91 strains). These results indicate that light organs of N. nuchalis flexion and postflexion larvae of 6.0 to 6.7 mm in NL are at an early stage of development and that inception of the symbiosis apparently occurs in flexion larvae of 6.0 to 6.5 mm in NL. Ontogeny of the light organ therefore apparently precedes acquisition of the symbiotic bacteria. Furthermore, bacterial populations in larval light organs near inception of the symbiosis are genetically diverse, like those of adult fish.

  9. Diversity within the Redeye Bass, Micropterus coosae (Perciformes: Centrarchidae) species group, with descriptions of four new species.

    PubMed

    Baker, Winston H; Blanton, Rebecca E; Johnston, Carol E

    2013-01-01

    The Redeye Bass, Micropterus coosae, was described from the Mobile River basin, Chattahoochee, and Savannah rivers in Alabama and Georgia, USA, by Hubbs and Bailey (1940). At that time the authors recognized significant variation in the Black Warrior River population, and noted that with further study this form may be recognized as a separate taxon. An examination of variation in morphology and mitochondrial DNA supported this observation, and highlighted additional species-level variation, resulting in descriptions of a total of four new species: Micropterus cahabae, new sp., restricted to the Cahaba River system; Micropterus tallapoosae, new sp., restricted to the Tallapoosa River system; Micropterus warriorensis, new sp., from the Black Warrior River system; and Micropterus chattahoochae, new sp., from the Chattahoochee River system. Micropterus coosae is restricted to the Coosa River system. The new species differ from each other and from M. coosae by a combination of pigmentation and scale count characteristics, development of the tooth patch, and divergence within the ND2 gene. While two of these species are relatively common in upland streams within their ranges, M. warriorensis, M. cahabae and M. chattahoochae are uncommon and may warrant protection.

  10. Molecular based phylogenetic species recognition in the genus Pampus (Perciformes: Stromateidae) reveals hidden diversity in the Indian Ocean.

    PubMed

    Divya, P R; Mohitha, C; Rahul, G Kumar; Rajool Shanis, C P; Basheer, V S; Gopalakrishnan, A

    2017-04-01

    Pomfrets (Genus Pampus) are commercially important fishes in the Indo Pacific region. The systematics of this genus is complicated due to morphological similarities between species. The silver pomfret from Indian waters has long been considered to be Pampus argenteus. The objective of the study was to utilize the mitochondrial COI gene to establish the molecular identity of the silver pomfret distributed in Indian waters and to resolve the phylogenetic relationships among Pampus species in the world based on sequence data in the NCBI database. Seven valid Pampus species are identified in this study. The mean genetic divergence value calculated between clades representing these species was 7.9%. The mean genetic distance between the so-called Pampus argenteus from Indian waters and sequences attributed to P. argenteus from the South China Sea, where the neotype of this species was collected, was found to be greater than 12%, strongly supporting the likelihood of the Indian species being distinct. The Indian Pampus species show very close affinity to P. cinereus, with inter species differences less than 2%. The taxonomic identity of the silver pomfret in India is also discussed here, in light of molecular and morphological evidence. Copyright © 2017 Elsevier Inc. All rights reserved.

  11. The complete mitochondrial genome of the southern purple-spotted gudgeon Mogurnda adspersa (Perciformes: Eleotridae) through pyrosequencing.

    PubMed

    Perini, Violeta da Rocha; de Carvalho, Daniel Cardoso; Beheregaray, Luciano Bellagamba; Prosdocimi, Francisco

    2016-01-01

    The complete mitochondrial genome of the Critically Endangered southern purple-spotted gudgeon, Mogurnda adspersa, was determined for the first time using 1/8 of a 454 pyrosequencing plate. The mitogenome was assembled using the bioinformatic software MIRA. The M. adspersa genome organization was very similar to most vertebrates, being 16,523 bp in length. It contained 13 protein-coding genes, 22 transfer RNA, 2 ribosomal RNA genes, and 1 non-coding control region. The current methodology was effective to assemble the whole mitogenome using 2008 mitochondrial reads representing 0.23% of total genomic reads produced and providing average mitogenome coverage of 17.4 reads per site. The whole mitogenome sequence provided here is deposited in NCBI with the accession number KJ130031 and may benefit systematics studies and conservation programs of M. adspersa.

  12. A comprehensive description and evolutionary analysis of 22 grouper (perciformes, epinephelidae) mitochondrial genomes with emphasis on two novel genome organizations.

    PubMed

    Zhuang, Xuan; Qu, Meng; Zhang, Xiang; Ding, Shaoxiong

    2013-01-01

    Groupers of the family Epinephelidae are a diverse and economically valuable group of reef fishes. To investigate the evolution of their mitochondrial genomes we characterized and compared these genomes among 22 species, 17 newly sequenced. Among these fishes we identified three distinct genome organizations, two of them never previously reported in vertebrates. In 19 of these species, mitochondrial genomes followed the typical vertebrate canonical organization with 13 protein-coding genes, 22 tRNAs, two rRNAs, and a non-coding control region. Differing from this, members of genus Variola have an extra tRNA-Ile between tRNA-Val and 16S rRNA. Evidence suggests that this evolved from tRNA-Val via a duplication event due to slipped strand mispairing during replication. Additionally, Cephalopholisargus has an extra tRNA-Asp in the midst of the control region, likely resulting from long-range duplication of the canonical tRNA-Asp through illicit priming of mitochondrial replication by tRNAs. Along with their gene contents, we characterized the regulatory elements of these mitochondrial genomes' control regions, including putative termination-associated sequences and conserved sequence blocks. Looking at the mitochondrial genomic constituents, rRNA and tRNA are the most conserved, followed by protein-coding genes, and non-coding regions are the most divergent. Divergence rates vary among the protein-coding genes, and the three cytochrome oxidase subunits (COI, II, III) are the most conserved, while NADH dehydrogenase subunit 6 (ND6) and the ATP synthase subunit 8 (ATP8) are the most divergent. We then tested the phylogenetic utility of this new mt genome data using 12 protein-coding genes of 48 species from the suborder Percoidei. From this, we provide further support for the elevation of the subfamily Epinephelinae to family Epinephelidae, the resurrection of the genus Hyporthodus, and the combination of the monotypic genera Anyperodon and Cromileptes to genus Epinephelus, and Aethaloperca to genus Cephalopholis.

  13. Projecting Range Limits with Coupled Thermal Tolerance - Climate Change Models: An Example Based on Gray Snapper (Lutjanus griseus) along the U.S. East Coast

    PubMed Central

    Hare, Jonathan A.; Wuenschel, Mark J.; Kimball, Matthew E.

    2012-01-01

    We couple a species range limit hypothesis with the output of an ensemble of general circulation models to project the poleward range limit of gray snapper. Using laboratory-derived thermal limits and statistical downscaling from IPCC AR4 general circulation models, we project that gray snapper will shift northwards; the magnitude of this shift is dependent on the magnitude of climate change. We also evaluate the uncertainty in our projection and find that statistical uncertainty associated with the experimentally-derived thermal limits is the largest contributor (∼ 65%) to overall quantified uncertainty. This finding argues for more experimental work aimed at understanding and parameterizing the effects of climate change and variability on marine species. PMID:23284974

  14. B-cell activating CpG ODN 1668 enhance the immune response of Pacific red snapper (Lutjanus peru) exposed to Vibrio parahaemolitycus.

    PubMed

    Cárdenas-Reyna, Tomás; Angulo, Carlos; Hori-Oshima, Sawako; Velázquez-Lizárraga, Esteban; Reyes-Becerril, Martha

    2016-09-01

    B-class CpG ODN 1668 is known to possess clear immunostimulatory properties. In this study, we investigated the potential ability of CpG ODN 1668 to enhance the immune response of Pacific red snapper exposed to Vibrio parahaemolyticus. Four different treatments were evaluated in Pacific red snapper: (1) stimulatory CpG ODN 1668, (2) stimulatory CpG ODN 1668 and V. parahaemolyticus, (3) exposure only to V. parahaemolyticus and (4) PBS. Samples were taken at 24, 72, 168 and 240 h of stimulation/infection. The results show that intraperitoneal injection of CpG-ODN 1668 enhanced the anti-protease, superoxide dismutase and catalase activities in serum. CpG ODN 1668 upregulated TLR9 and IgM gene expression in head-kidney, intestine and skin, with higher expression in head-kidney. A higher correlation was observed between TLR9 and IgM in head-kidney and intestine. Finally, no histopathological damages were observed in fish stimulated with CpG ODN 1668. In contrast, melanomacrophages-like structures were present in higher numbers in infected fish. Taken together, these results indicate that CpG ODN 1668 activates innate immune response and upregulate the TLR9 and IgM-mediated immune response. These results may be exploited for the control of Vibriosis in farmed Pacific red snapper.

  15. Debaryomyces hansenii up regulates superoxide dismutase gene expression and enhances the immune response and survival in Pacific red snapper (Lutjanus peru) leukocytes after Vibrio parahaemolyticus infection.

    PubMed

    Angulo, Carlos; Maldonado, Minerva; Delgado, Karen; Reyes-Becerril, Martha

    2017-06-01

    Application of yeast is increasing to improve welfare and promotes growth in aquaculture. The halotolerant yeast Debaryomyces hansenii is normally a non-pathogenic yeast with probiotic properties and potential source of antioxidant enzymes as superoxide dismutase. Here, first, we characterized the sequence features of MnSOD and icCu/ZnSOD from Pacific red snapper, and second, we evaluated the potential antioxidant immune responses of the marine yeast Debaryomyces hansenii strain CBS004 in leukocytes which were then subjected to Vibrio parahaemolyticus infection. In silico analysis revealed that LpMnSOD consisted of 1186 bp, with an ORF of 678 bp encoding a 225 amino acid protein and LpicCu/ZnSOD consisted of 1090 bp in length with an ORF of 465 bp encoding a 154 amino acid protein. Multiple alignment analyzes revealed many conserved regions and active sites among its orthologs. In vitro assays using head-kidney and spleen leukocytes immunostimulated with D. hansenii and zymosan in response to V. parahaemolyticus infection reveled that D. hansenii strain CBS004 significantly increased transcriptions of MnSOD and icCu/ZnSOD genes. Flow cytometry assay showed that D. hansenii was able to inhibit apoptosis caused by V. parahaemolyticus in the Pacific red snapper leukocytes and enhanced the phagocytic capacity in head-kidney leukocytes. Immunological assays reveled an increased in superoxide dismutase and peroxidase activities, as well as, in nitric oxide production and reactive oxygen species production (respiratory burst) in fish stimulated with D. hansenii. Finally, our results. These results strongly support the idea that marine yeast Debaryomyces hansenii strain CBS004 can stimulate the antioxidant immune mechanism in head-kidney and spleen leukocytes. Copyright © 2017 Elsevier Ltd. All rights reserved.

  16. Temporal and spatial comparisons of the reproductive biology of northern Gulf of Mexico (USA) red snapper (Lutjanus campechanus) collected a decade apart.

    PubMed

    Kulaw, Dannielle H; Cowan, James H; Jackson, Melissa W

    2017-01-01

    In studies done a decade apart, we provide evidence of a recent shift toward a slower progression to sexual maturity as well as reduced egg production, especially among young, small female red snapper, in the Gulf of Mexico (Gulf). Slower maturation rates (among fish ≤6 years old), lower GSI values and decreased spawning frequency were observed, and were especially pronounced in the northwestern Gulf. Furthermore, an Index of Reproductive Importance showed that young fish (ages 2-7) are contributing far less to the spawning stock in recent years, while older fish (>8 years) are contributing more, when compared to fish from the same age groups sampled in the previous decade. Coincident with these changes in reproductive output, fishing pressure has steadily declined gulf-wide, and spawning stock biomass and spawning potential ratio have increased. Thus, it is possible that the age structure of the red snapper stock is becoming less truncated, or that reproductive effort observed is due to the temporary influence of recent strong year classes produced in 2004 and 2006 as they begin to reach full reproductive potential. If the latter is true, careful documentation of the stock's reproductive dynamics during a time of population growth provides new understanding at the meta-population spatial and decadal temporal scales. In contrast, if the former is true, a truncated age structure due to overharvest can limit the productivity of the Gulf red snapper stock. In addition, we have learned that red snapper females in the northwestern Gulf collected on natural reefs and banks have much higher reproductive output than those on artificial reefs in the form of standing and toppled oil and gas platforms, thus making the need to know the relative abundance of females found on these disparate habitats an important next step toward better-understanding factors impacting the reproductive dynamics of this species.

  17. The effects of temperature on gastric emptying time of malabar blood snapper (Lutjanus malabaricus, Bloch & Schneider 1801) using X-radiography technique

    SciTech Connect

    Mazumder, Sabuj Kanti; Ghaffar, Mazlan Abd.; Das, Simon Kumar

    2015-09-25

    Water temperature can influence the metabolic rate of ectothermal animal such as, fish. This study aims to investigate the gastric emptying time (GET) of malabar blood snapper (L. malabaricus) at different temperature (22, 26, 30, 34°C) fed with commercial diet pellet. The gastric emptying times at different temperatures were determined X-radiographically, using barium sulfate (BaSO{sub 4}) as a contrast medium food marker. The food marker and X-radiography showed that initial voidance of fecal matter began 4-8 h after feeding at all temperature. The fastest GET (18 h) was obsereved at 30°C, whereas the longest (28 h) GET was seen at 22°C. GET was increased with increasing temperature up to 30°C whereas decreased at extreme (34°C) and stress temperature (22°C). The findings of this study suggest to culture L. malabaricus at 26-30°C with commercial diet pellet as this temperature ranges escalate the faster digestion process which may contribute faster growth rate of this commercially important fish species. Overall, the data obtained from this study may have important consequences for optimization of commercial production of malabar blood snapper.

  18. The effects of temperature on gastric emptying time of malabar blood snapper (Lutjanus malabaricus, Bloch & Schneider 1801) using X-radiography technique

    NASA Astrophysics Data System (ADS)

    Mazumder, Sabuj Kanti; Ghaffar, Mazlan Abd.; Das, Simon Kumar

    2015-09-01

    Water temperature can influence the metabolic rate of ectothermal animal such as, fish. This study aims to investigate the gastric emptying time (GET) of malabar blood snapper (L. malabaricus) at different temperature (22, 26, 30, 34°C) fed with commercial diet pellet. The gastric emptying times at different temperatures were determined X-radiographically, using barium sulfate (BaSO4) as a contrast medium food marker. The food marker and X-radiography showed that initial voidance of fecal matter began 4-8 h after feeding at all temperature. The fastest GET (18 h) was obsereved at 30°C, whereas the longest (28 h) GET was seen at 22°C. GET was increased with increasing temperature up to 30°C whereas decreased at extreme (34°C) and stress temperature (22°C). The findings of this study suggest to culture L. malabaricus at 26-30°C with commercial diet pellet as this temperature ranges escalate the faster digestion process which may contribute faster growth rate of this commercially important fish species. Overall, the data obtained from this study may have important consequences for optimization of commercial production of malabar blood snapper.

  19. Temporal and spatial comparisons of the reproductive biology of northern Gulf of Mexico (USA) red snapper (Lutjanus campechanus) collected a decade apart

    PubMed Central

    Kulaw, Dannielle H.; Jackson, Melissa W.

    2017-01-01

    In studies done a decade apart, we provide evidence of a recent shift toward a slower progression to sexual maturity as well as reduced egg production, especially among young, small female red snapper, in the Gulf of Mexico (Gulf). Slower maturation rates (among fish ≤6 years old), lower GSI values and decreased spawning frequency were observed, and were especially pronounced in the northwestern Gulf. Furthermore, an Index of Reproductive Importance showed that young fish (ages 2–7) are contributing far less to the spawning stock in recent years, while older fish (>8 years) are contributing more, when compared to fish from the same age groups sampled in the previous decade. Coincident with these changes in reproductive output, fishing pressure has steadily declined gulf-wide, and spawning stock biomass and spawning potential ratio have increased. Thus, it is possible that the age structure of the red snapper stock is becoming less truncated, or that reproductive effort observed is due to the temporary influence of recent strong year classes produced in 2004 and 2006 as they begin to reach full reproductive potential. If the latter is true, careful documentation of the stock’s reproductive dynamics during a time of population growth provides new understanding at the meta-population spatial and decadal temporal scales. In contrast, if the former is true, a truncated age structure due to overharvest can limit the productivity of the Gulf red snapper stock. In addition, we have learned that red snapper females in the northwestern Gulf collected on natural reefs and banks have much higher reproductive output than those on artificial reefs in the form of standing and toppled oil and gas platforms, thus making the need to know the relative abundance of females found on these disparate habitats an important next step toward better-understanding factors impacting the reproductive dynamics of this species. PMID:28355239

  20. Geographical distribution of protozoan and metazoan parasites of farmed Nile tilapia Oreochromis niloticus (L.) (Perciformes: Cichlidae) in Yucatán, México.

    PubMed

    Paredes-Trujillo, Amelia; Velázquez-Abunader, Iván; Torres-Irineo, Edgar; Romero, David; Vidal-Martínez, Víctor Manuel

    2016-02-03

    In Yucatán State, southern México, as in many other parts of the world where tilapia has been introduced for aquaculture, the deficient application of management measures has led to the establishment of non-native parasites. The aims of this study were to describe the geographical distribution of protozoan and helminth parasites of farmed Nile tilapia Oreochromis niloticus (L.) throughout the Yucatán and to examine the potential statistical associations of the prevalence and mean abundance of these parasites with management and environmental variables. All 29 Nile tilapia farms currently operating in Yucatán were surveyed. Maps were created to describe the geographical location of the parasites infecting Nile tilapia at each farm. We evaluated the statistical associations of management and environmental variables with the mean abundance values of each parasite species using a multivariate redundancy analysis (RDA) and generalized additive models (GAM). We also used Ripley's K to determine whether there were significant clusters of the mean abundance of particular parasite species in specific regions of the Yucatán State. A total of 580 O. niloticus were examined, and 11 species of parasites were recorded. Cichlidogyrus sclerosus was the most frequent and abundant parasite at all 29 farms, whereas Gyrodactylus cichlidarum was found in 26 of the 29 farms. The RDA showed that the most important predictors were the concentration of nitrites and ammonium and the water exchange rate. The GAM showed the significant effect of the tank capacity, no use of quarantine area and no use of prophylactic treatments on the mean abundance of G. cichlidarum. The geographical distribution patterns of the mean abundance of most parasite species exhibited clustering near to the coast of Yucatán. Two groups of farms were distinguished: (i) farms with medium to high technology, where the most frequent and abundant parasite was G. cichlidarum, and (ii) farms with low technology, where the most frequent and abundant parasite was C. sclerosus. Good biosecurity practices such as the use of quarantine and prophylactic treatments prior to the introduction of infected Nile tilapia to the farms are recommended to avoid the establishment of parasites such as G. cichlidarum in farms.

  1. Taxonomic review of the species of Mugil (Teleostei: Perciformes: Mugilidae) from the Atlantic South Caribbean and South America, with integration of morphological, cytogenetic and molecular data.

    PubMed

    Menezes, Naércio A; Nirchio, Mauro; De Oliveira, Cláudio; Siccharamirez, Raquel

    2015-01-10

    Analysis of morphological, molecular and cytological data helped to define and more precisely characterize the species of Mugil from the Atlantic coasts of South Caribbean and South America, allowing a correction of prior misidentifications and distributional ranges. A new species from Venezuela is described and all the species from the area are redescribed. It is demonstrated that the apparent similarity in morphological traits, which contradicted the results from recent molecular studies, is the result of the misuse of traditional morphological characters, and thus both the molecular and cytological data instead are congruent with the morphological differences that are found among mullet species. The presence of Mugil hospes Jordan & Culver in the western south Atlantic is refuted based on the comparison of type material of this species with specimens from this area that also indicated a very significant morphological difference, what on the other hand justifies the recognition of these specimens as Mugil brevirostris (Ribeiro). The distribution of Mugil incilis Hancock is restricted and the similarities among the species formerly depicted in a prior dendrogram is modified following the inclusion of recently obtained molecular data for Mugil curvidens Valenciennes.

  2. Co-located 18S/5S rDNA arrays: an ancient and unusual chromosomal trait in Julidini species (Labridae, Perciformes)

    PubMed Central

    Amorim, Karlla Danielle Jorge; Cioffi, Marcelo de Bello; Bertollo, Luiz Antonio Carlos; Soares, Rodrigo Xavier; de Souza, Allyson Santos; da Costa, Gideão Wagner Werneck Felix; Molina, Wagner Franco

    2016-01-01

    Abstract Wrasses (Labridae) are extremely diversified marine fishes, whose species exhibit complex interactions with the reef environment. They are widely distributed in the Indian, Pacific and Atlantic oceans. Their species have displayed a number of karyotypic divergent processes, including chromosomal regions with complex structural organization. Current cytogenetic information for this family is phylogenetically and geographically limited and mainly based on conventional cytogenetic techniques. Here, the distribution patterns of heterochromatin, GC-specific chromosome regions and Ag-NORs, and the organization of 18S and 5S rDNA sites of the Atlantic species Thalassoma noronhanum (Boulenger, 1890), Halichoeres poeyi (Steindachner, 1867), Halichoeres radiatus (Linnaeus, 1758), Halichoeres brasiliensis (Bloch, 1791) and Halichoeres penrosei Starks, 1913, belonging to the tribe Julidini were analyzed. All the species exhibited 2n=48 chromosomes with variation in the number of chromosome arms among genera. Thalassoma noronhanum has 2m+46a, while species of the genus Halichoeres Rüppell, 1835 share karyotypes with 48 acrocentric chromosomes. The Halichoeres species exhibit differences in the heterochromatin distribution patterns and in the number and distribution of 18S and 5S rDNA sites. The occurrence of 18S/5S rDNA syntenic arrangements in all the species indicates a functionally stable and adaptive genomic organization. The phylogenetic sharing of this rDNA organization highlights a marked and unusual chromosomal singularity inside the family Labridae. PMID:28123678

  3. Review of the Acanthopagrus latus complex (Perciformes: Sparidae) with descriptions of three new species from the Indo-West Pacific Ocean.

    PubMed

    Iwatsuki, Y

    2013-07-01

    Acanthopagrus latus, long considered a single valid Indo-West Pacific Ocean species, characterized by having yellow pelvic, anal and caudal fins, is reviewed and separated into A. latus (east Asian shelf) and Acanthopagrus longispinnis (Bengal Bay), and three new species: Acanthopagrus morrisoni sp. nov. (north-western Australia), Acanthopagrus arabicus sp. nov. [Middle East (except for the Red Sea) to coasts of Iran and Pakistan, and western Indian coast] and Acanthopagrus sheim sp. nov. (The Gulf). Although A. latus as redefined considerably varies in morphology and colouration, it can be recognized as a discrete east Asian endemic, with the following nominal species being junior synonyms: Chrysophrys auripes, Chrysophrys xanthopoda, Chrysophrys rubroptera and Sparus chrysopterus. Chrysophrys novaecaledoniae, known only from the holotype (type locality: Nouméa, New Caledonia), is a questionable junior synonym of A. latus, the lack of subsequent collections suggesting that the type locality is erroneous. Acanthopagrus longispinnis is differentiated from the other species in the complex by consistently having 12 dorsal-fin spines and a much larger second anal-fin spine, 21-26% (mean 23%) of standard length (LS ) (v. 14-24%, mean 18-21% in the other four species). Acanthopagrus morrisoni sp. nov. has the entire caudal fin yellow with a wide black posterior margin (persisting in preserved specimens) and consistently 3 ½ scale rows between the fifth dorsal-fin spine base and the lateral line. Acanthopagrus sheim sp. nov. has the pelvic, anal and lower caudal fins vivid yellow, with two (rarely three) small black blotches on the lower inter-radial membranes between the spinous and soft dorsal-fin rays. Acanthopagrus arabicus sp. nov. consistently has 4 ½ scale rows between the fifth dorsal-fin spine base and the lateral line, whereas A. latus always has black streaks proximally on the inter-radial membranes between the yellow anal-fin rays. A neotype and lectotye, respectively, are designated for A. latus and A. longispinnis. The p-distance (net nucleotide substitutions per site) of partial mitochondrial 16s ribosomal RNA genes (538 bp) among the above species (except A. longispinnis) and three other congeners (Acanthopagrus berda, Acanthopagrus pacificus and Acanthopagrus bifasciatus) strongly indicates that each is a distinct species. A key is provided for the 20 species of Acanthopagrus currently known from the Indo-West Pacific Ocean.

  4. Homogeneous Nature of Malaysian Marine Fish Epinephelus fuscoguttatus (Perciformes; Serranidae): Evidence Based on Molecular Markers, Morphology and Fourier Transform Infrared Analysis.

    PubMed

    Nurdalila, A'wani Aziz; Bunawan, Hamidun; Kumar, Subbiah Vijay; Rodrigues, Kenneth Francis; Baharum, Syarul Nataqain

    2015-07-02

    Taxonomic confusion exists within the genus Epinephelus due to the lack of morphological specializations and the overwhelming number of species reported in several studies. The homogenous nature of the morphology has created confusion in the Malaysian Marine fish species Epinephelus fuscoguttatus and Epinephelus hexagonatus. In this study, the partial DNA sequence of the 16S gene and mitochondrial nucleotide sequences of two gene regions, Cytochrome Oxidase Subunit I and III were used to investigate the phylogenetic relationship between them. In the phylogenetic trees, E. fuscoguttatus was monophyletic with E. hexagonatus species and morphology examination shows that no significant differences were found in the morphometric features between these two taxa. This suggests that E. fuscoguttatus is not distinguishable from E. hexagonatus species, and that E. fuscoguttatus have been identified to be E. hexagonatus species is likely attributed to differences in environment and ability to camouflage themselves under certain conditions. Interestingly, this finding was also supported by Principal Component Analysis on Attenuated Total Reflectance-Fourier-transform Infrared (ATR-FTIR) data analysis. Molecular, morphological and meristic characteristics were combined with ATR-FTIR analysis used in this study offer new perspectives in fish species identification. To our knowledge, this is the first report of an extensive genetic population study of E. fuscoguttatus in Malaysia and this understanding will play an important role in informing genetic stock-specific strategies for the management and conservation of this highly valued fish.

  5. On the colour types in Lycodes nakamurae (Tanaka, 1914) and species composition of the subgenus Furcimanus (Perciformes: Zoarcidae: Lycodes) in the sea of Japan.

    PubMed

    Saveliev, Pavel A; Balanov, Andrey A; Kukhlevskii, Andrey D

    2014-05-30

    Two colour types were revealed in a zoarcid fish of the subgenus Furcimanus, genus Lycodes, in the Sea of Japan. A comparison of morphometric, meristic and genetic characters in dark coloured and light coloured individuals suggests that the two colour morphs represent a single species, determined to be Lycodes nakamurae (Tanaka, 1914). Variability in colouration within L. nakamurae and a lack of morphological or molecular characters distinguishing L. nakamurae from L. nishimurai Shinohara & Shirai, 2005 suggest that the latter should be considered a synonym of L. nakamurae (Tanaka, 1914). A record of L. pectoralis in the waters of the Republic of Korea is regarded as a misidentification. Thus, we conclude that only one species of the Lycodes subgenus Furcimanus, L. nakamurae, with dark and light colour morphs as well as specimens of intermediate colouration, inhabits the Sea of Japan.

  6. First record of the Indo-Pacific areolate grouper Epinephelus areolatus (Forsskål, 1775) (Perciformes: Epinephelidae) in the Mediterranean Sea.

    PubMed

    Rothman, Shevy B S; Stern, Nir; Goren, Menachem

    2016-01-25

    The number of alien species in the Mediterranean Sea is steadily increasing and it seems that the pace has been accelerating since the turn of the century (Galil et al. 2014). In 2015 alone five additional fish species have been reported, Epinephelus geoffroyi (Klunzinger, 1870) (Golani et al. 2015); Stolephorus indicus (van Hasselt, 1823) (Fricke et al. 2015); Sardinella gibbosa (Bleeker, 1849) (Stern et al. 2015); Mobula japanica (Müller & Henle, 1841) (Capapé et al. 2015); and Cryptocentrus caeruleopunctatus (Rüppell, 1830) (Rothman & Goren 2015). Among the ca. 100 alien fish species reported from the Mediterranean to date (Galil & Goren 2014), five Indo-Pacific species belong to the genus Epinephelus Bloch, 1793: Epinephelus coioides (Hamilton, 1822), Epinephelus fasciatus (Forsskål, 1775), Epinephelus malabaricus (Bloch and Schneider, 1804) Epinephelus merra Bloch, 1793 (Golani et al. 2013a) and Epinephelus geoffroyi (Klunzinger, 1870) (Golani et al. 2015). Additional alien Epinephelus species reported from the Mediterranean are excluded for various reasons (Golani et al. 2013b). Here we report the finding of a sixth Indo-Pacific species of this genus along the Mediterranean coast of Israel.

  7. Revision of Metahaliotrema Yamaguti, 1953 (Monogenoidea: Dactylogyridae), with new and previously described species from the spotted scat Scatophagus argus (Linnaeus) (Perciformes: Scatophagidae) in Vietnam.

    PubMed

    Kritsky, Delane C; Nguyen, Ha Van; Ha, Ngo Duy; Heckmann, Richard A

    2016-05-01

    An emended diagnosis of Metahaliotrema Yamaguti, 1953 (Monogenoidea: Dactylogyridae) is provided based on specimens of six species collected from the spotted scat Scatophagus argus (Linnaeus) (Scatophagidae) in Vietnam: M. scatophagi Yamaguti, 1953 (type-species); M. cf. yamagutii Mizelle & Price, 1964; M. mizellei Venkatanarasaiah, 1981; M. kulkarnii Venkatanarasaiah, 1981; M. ypsilocleithrum n. sp.; and M. similis n. sp. Methaliotrema filamentosum Venkatanarasaiah, 1981 from the whipfin silver-biddy Gerres filamentosus Cuvier (Gerreidae) is included as the only other valid member of the genus. Metahaliotrema arii Yamaguti, 1953 from an ariid catfish is considered incertae sedis within the Dactylogyridae; and Metahaliotrema srivastavai Singh & Agarwal, 1994 from a bagrid catfish is transferred to Chauhanellus Bychowsky & Nagibina, 1969 as Chauhanellus srivastavai (Singh & Agarwal, 1994) n. comb. Metahaliotrema geminatohamula Pan, Ding & Zhang, 1995 from spotted scat in China is determined to be a junior subjective synonym of M. scatophagi. The two new species and M. scatophagi, M. mizellei, and M. kulkarnii are described or redescribed based on specimens from Vietnam.

  8. Northernmost record of a poorly known tuskfish, Choerodon margaritiferus (Perciformes: Labridae), from southern Japan, and first description of a female.

    PubMed

    Miyamoto, Kei; Nonaka, Ai; Oka, Shin-Ichiro

    2015-08-26

    We describe 5 specimens of Choerodon margaritiferus Fowler and Bean, 1928 (94.5-107.0 mm standard length, 1 male and 4 females) collected near Okinawa Island, southern Japan. This is the northernmost record of this species and the first record of it in Japanese waters; until now, C. margaritiferus was recorded only from the western central Pacific. This species was originally described on the basis of 1 male specimen, and all additional specimens described in the literature were also male. Morphological characters of both sexes are described, and new diagnostic characters of the female are presented.

  9. Complete mitochondrial genome of the urohaze-goby Glossogobius olivaceus (Perciformes, Gobiidae) and structure comparison of light strand replication origin in Gobioidei.

    PubMed

    Sun, Yuena; Wei, Tao; Jin, Xiaoxiao; Qin, Yu; Xu, Tianjun

    2015-01-01

    The genome of Glossogobius olivaceus (G. olivaceus) was 16,568 bp in length with a standard set of 13 protein-coding genes, 22 transfer RNA genes (tRNAs), 2 ribosomal RNA genes (rRNAs) and 2 non-coding region (the control region and origin of the light strand replication). The conserved motif 5'-GCCGG-3' was determined in the origin of light strand replication of G. olivaceus. The G. olivaceus mitogenome base composition was: T 25.65%, C 29.82% A 27.17% and G 17.36%, with a slight A+T bias of 52.82%, which was similar to most of the vertebrate mitogenomes. The mitochondrial genome of G. olivaceus had common features regarding gene arrangement and tRNA structures compared with those of other bony fishes.

  10. Southeast Asian mouth-brooding Betta fighting fish (Teleostei: Perciformes) species and their phylogenetic relationships based on mitochondrial COI and nuclear ITS1 DNA sequences and analyses

    PubMed Central

    Panijpan, Bhinyo; Kowasupat, Chanon; Laosinchai, Parames; Ruenwongsa, Pintip; Phongdara, Amornrat; Senapin, Saengchan; Wanna, Warapond; Phiwsaiya, Kornsunee; Kühne, Jens; Fasquel, Frédéric

    2014-01-01

    Fighting fish species in the genus Betta are found in several Southeast Asian countries. Depending on the mode of paternal care for fertilized eggs and hatchlings, various species of the betta fish are classified as mouth brooders or nest builders whose members in turn have been grouped according to their similarities mainly in morphology. The mouth brooders as well as some nest builders involved in the present study include fishes discovered and identified subsequent to previous reports on species groupings and their positions on phylogenetic trees based on DNA sequences that differ from those used by us in this study. From the mitochondrial COI gene and nuclear ITS1 gene sequences and more accurate analyses we conclude that the following members of the mouth-brooding pairs, named differently previously, are virtually identical, viz the Betta prima–Betta pallida pair and Betta ferox–Betta apollon pair. The Betta simplex, hitherto believed to be one species, could possibly be genetically split into 2 distinct species. In addition, several other established type-locality fishes could harbor cryptic species as judged by genetic differences. Assignments of fish species to groups reported earlier may have to be altered somewhat by the present genetic findings. We propose here a new Betta fish phylogenetic tree which, albeit being similar to the previous ones, is clearly different from them. Our gene-based evidence also leads to assignments of some fishes to new species groups and alters the positions of some species on the new phylogenetic tree, thus implying different ancestral relationships. PMID:25606468

  11. Biodiversity of the Betta smaragdina (Teleostei: Perciformes) in the northeast region of Thailand as determined by mitochondrial COI and nuclear ITS1 gene sequences☆

    PubMed Central

    Kowasupat, Chanon; Panijpan, Bhinyo; Laosinchai, Parames; Ruenwongsa, Pintip; Phongdara, Amornrat; Wanna, Warapond; Senapin, Saengchan; Phiwsaiya, Kornsunee

    2014-01-01

    In Thailand, there are currently five recognized species members of the bubble-nesting Betta genus, namely Betta splendens, B. smaragdina, B. imbellis, B. mahachaiensis and B. siamorientalis. In 2010, we indicated the possibility, based on COI barcoding evidence, that there might be two additional species, albeit cryptic, related to the type-locality B. smaragdina in some provinces in the northeast of Thailand. In the present study, after a more extensive survey of the northeast, and phylogenetic analyses based on COI and ITS1 sequences, the B. smaragdina group may be composed of at least 3 cryptic species members. The phylogenetic positions of these B. smaragdina group members in the bubble-nesting bettas' tree together with those of their congeners have been consolidated by better DNA sequence quality and phylogenetic analyses. With a better supported tree, the species statuses of B. siamorientalis and the Cambodian B. smaragdina-like fish, B. stiktos, are also confirmed. PMID:25606392

  12. Biodiversity of the Betta smaragdina (Teleostei: Perciformes) in the northeast region of Thailand as determined by mitochondrial COI and nuclear ITS1 gene sequences.

    PubMed

    Kowasupat, Chanon; Panijpan, Bhinyo; Laosinchai, Parames; Ruenwongsa, Pintip; Phongdara, Amornrat; Wanna, Warapond; Senapin, Saengchan; Phiwsaiya, Kornsunee

    2014-12-01

    In Thailand, there are currently five recognized species members of the bubble-nesting Betta genus, namely Betta splendens, B. smaragdina, B. imbellis, B. mahachaiensis and B. siamorientalis. In 2010, we indicated the possibility, based on COI barcoding evidence, that there might be two additional species, albeit cryptic, related to the type-locality B. smaragdina in some provinces in the northeast of Thailand. In the present study, after a more extensive survey of the northeast, and phylogenetic analyses based on COI and ITS1 sequences, the B. smaragdina group may be composed of at least 3 cryptic species members. The phylogenetic positions of these B. smaragdina group members in the bubble-nesting bettas' tree together with those of their congeners have been consolidated by better DNA sequence quality and phylogenetic analyses. With a better supported tree, the species statuses of B. siamorientalis and the Cambodian B. smaragdina-like fish, B. stiktos, are also confirmed.

  13. Parasciadicleithrum octofasciatum n. gen., n. sp. (Monogenoidea: Dactylogyridae), parasite of Rocio octofasciata (Regan) (Cichlidae: Perciformes) from Mexico characterised by morphological and molecular evidence.

    PubMed

    Mendoza-Palmero, Carlos A; Blasco-Costa, Isabel; Hernández-Mena, David; Pérez-Ponce de León, Gerardo

    2017-04-01

    Based on an integrative taxonomic approach, combining morphological characters and partial sequences of the 28S rRNA gene, a new genus and species, Parasciadicleithrum octofasciatum, is proposed to accommodate dactylogyrids infecting the gills of Rocio octofasciata (Cichlidae) from a tributary of the Lacantún River basin, Chiapas State, southern Mexico. Morphologically, the new genus resembles members of Sciadicleithrum (Dactylogyridae) in the presence of membranes on the anterior margins of both ventral and dorsal haptoral bars. However, Parasciadicleithrum octofasciatum n. sp. shows morphological differences with respect to a subset of species of Sciadicleithrum occurring in the same host group and geographic area, such as the body size and gonad dimensions (length and width of germarium and testis, respectively, smaller in the new genus). Phylogenetic analyses performed herein based on Maximum Likelihood and Bayesian Inference criteria, showed that Parasciadicleithrum octofasciatum n. sp. represents an undescribed taxon morphologically very similar to species of Sciadicleithrum from which it can be consistently distinguished on the basis of molecular data. P. octofasciatum n. sp. appeared genetically distant from Sciadicleithrum spp. and nested with dactylogyrid parasites of African cichlids. Parasciadicleithrum n. gen. showed divergence levels in the 28S rDNA sequences of 25-26% with respect to Sciadicleithrum spp. Therefore, on the basis of molecular evidence mainly, and morphological differences highlighted above, the erection of the new genus is proposed. The evolutionary and ecological factors that may have influenced the associations between Neotropical cichlids and their dactylogyrid parasites are briefly discussed.

  14. Population parameters of the Pacific flagfin mojarra Eucinostomus currani (Perciformes: Gerreidae) captured by shrimp trawling fishery in the Gulf of California.

    PubMed

    López-Martínez, Juana; Rodríguez-Romero, Jesús; Hernández-Saavedra, Norma Y; Herrera-Valdivia, Eloisa

    2011-06-01

    Shrimp trawling fishery in the Gulf of California captures a wide variety of non-target species of fish, crustaceans, and mollusks that are relatively unknown. The Pacific flagfin mojarra Eucinostomus currani is a frequently found species in these catches, nevertheless, nothing is currently known about its population dynamics. To contribute to the knowledge on this fish species, we studied the size structure, growth, mortality, and the recruitment pattern during the 2004-2005 seasons. A total of 6,078 mojarra were captured from 350 samples, with minimum and maximum lengths of 4.5 cm and a maximum of 21.0 cm. The average total length of the four major cohorts was 11.4, 13.7, 15.6 and 18.0 cm, corresponding to ages 0.9, 1.2, 1.6 and 2.2 years, respectively, being the most abundant the 1.2 year-old group. The instant growth coefficient indicated moderate growth rates (K(s) = 0.81/year, K(E) = 0.85/year), corresponding to individuals living between 3.5 to 3.7 years. The estimated asymptotic lengths was L (infinity) = 21.8 cm. In general, the population could be considered healthy: natural mortality (M = 1.53/year); total mortality (Z = 2.73/year); condition factor (K = 0.01072); fishery mortality (F = 1.2/year) and exploitation rate (E = 0.43/year). The maximum reproduction period almost coincided with the closed season for shrimp fishing (March to August), thus we concluded that survival of the species is ensured because reproduction is indirectly protected.

  15. Mulloidichthys flavolineatus flavicaudus Fernandez-Silva & Randall (Perciformes, Mullidae), a new subspecies of goatfish from the Red Sea and Arabian Sea.

    PubMed

    Fernandez-Silva, Iria; Randall, John E; Golani, Daniel; Bogorodsky, Sergey V

    2016-01-01

    The number of goatfish species has increased recently, thanks in part to the application of molecular approaches to the taxonomy of a family with conservative morphology and widespread intraspecific color variation. A new subspecies Mulloidichthys flavolineatus flavicaudus Fernandez-Silva & Randall is described from the Red Sea and Arabian Sea, including Socotra and Gulf of Oman. It is characterized by a yellow caudal fin, 25-28 gill rakers, and 37-38 lateral-line scales and it is differentiated from nominal subspecies Mulloidichthys flavolineatus flavolineatus by 1.7% sequence divergence at the mitochondrial cytochrome b gene. The morphometric examination of specimens of Mulloidichthys flavolineatus flavolineatus revealed variation in head length, eye diameter, and barbel length, in western direction from the Hawaiian Islands, South Pacific, Micronesia, and the East Indies to the Indian Ocean. The population of Mulloidichthys flavolineatus flavicaudus subsp. n. in the Gulf of Aqaba differs from that of the remaining Red Sea by shorter barbels, smaller eyes, shorter head, and shorter pelvic fins. We present a list of 26 endemic fishes from the Gulf of Aqaba and discuss the probable basis for the endemism in the light of the geological history of this region.

  16. Three species of opisthomonorchiine monorchiids (Digenea) in Carangoides spp. (Perciformes: Carangidae) from off New Caledonia, with a description of Opisthomonorchis dinema n. sp.

    PubMed

    Bray, Rodney A; Justine, Jean-Lou

    2013-06-01

    Three opisthomonorchiinae species are described from fishes obtained at the Fish Market in Nouméa, New Caledonia. Opisthomonorchis dinema n. sp. from Carangoides dinema Bleeker differs from the other recognised species in the genus by the long recurved genital atrium, arcing anteriorly. Also described are Opisthomonorchis carangis Yamaguti, 1952 from Carangoides sp. and Pseudopisthomonorchis thapari (Varma & Singh, 1979) n. comb. for Opisthomonorchis thapari Varma & Singh, 1979 from Carangoides chrysophrys (Cuvier). The features distinguishing Opisthomonorchis Yamaguti, 1952 and Pseudopisthomonorchis Madhavi, 1974 are discussed.

  17. Three species of Calydiscoides (Monogenea: Diplectanidae) from five Lethrinus spp. (Lethrinidae: Perciformes) off New Caledonia, with a description of Calydiscoides terpsichore sp. n.

    PubMed

    Rascalou, Guilhem; Justine, Jean-Lou

    2007-09-01

    Numerous specimens of Calydiscoides spp. from Lethrinus nebulosus and L. harak, and fewer specimens from L. lentjan, L. ravus and L. obsoletus, collected off New Caledonia, South Pacific, were examined. Three species of Calydiscoides were recognized. Calydiscoides difficilis (Yamaguti, 1953) Young, 1969 was generally the most abundant species on the five hosts. Specimens of C. difficilis were measured and compared to the type specimens (from Indonesia) and museum vouchers (from Australia and the Arabian Gulf). The morphology of the massive male copulatory organ and of haptoral sclerotized parts was similar, but specimens from the same host and from different hosts showed wide morphometric variations; it is concluded that C. difficilis has a high degree of variability, although the possibility of a species complex remains. Calydiscoides duplicostatus (Yamaguti, 1953) Young, 1969 was found on L. nebulosus, L. harak and L. lentjan; specimens were homogeneous in the three hosts and similar to the type specimens. Calydiscoides terpsichore sp. n. is described from L. nebulosus (type host) and L. harak and was uncommon in both fish. The new species, a member of the 'australis group', is characterized by its Y-shaped male copulatory organ, with the left branch bearing a trifurcated secondary branch. Lethrinus nebulosus and L. harak shared the 3 species of Calydiscoides studied here, a fact probably related with their close phylogenetic relationships. Among the 15 species of Lethrinus present in New Caledonia, 11 were examined and 9 species harboured 1-3 species of Calydiscoides each. Only 7 species of Calydiscoides were found, due to their stenoxenous specificity. Each species of Calydiscoides has 1 to 5 hosts.

  18. A digenean parasite in a mudskipper: Opegaster ouemoensis sp. n. (Digenea: Opecoelidae) in Periophthalmus argentilineatus Valenciennes (Perciformes: Gobiidae) in the mangroves of New Caledonia.

    PubMed

    Bray, Rodney A; Justine, Jean-Lou

    2013-02-01

    Opegaster ouemoensis sp. n. is described from Periophthalmus argentilineatus Valenciennes (Gobiidae). Distinctive features included the weak or undetectable papillae of the ventral sucker and the small, but distinct cirrus-sac. The new species is compared with 25 marine species of Opegaster for which a table of measurements and ratios is presented. The new combination Opegaster queenslandicus (Aken'Ova, 2007) (originally in Opecoelus) is formed. Fifteen mudskippers were intensively examined for parasites; larval anisakid nematodes and acanthocephalans were found, but no monogeneans, cestodes, copepods, isopods, hirudineans or adult nematodes. A brief summary of the helminth parasites of mudskippers is included.

  19. Monogeneans of the grouper Epinephelus tauvina (Perciformes, Serranidae) off Moorea, French Polynesia, with a description of Pseudorhabdosynochus pai n. sp. (Monogenea: Diplectanidae).

    PubMed

    Justine, Jean-Lou; Vignon, Matthias

    2009-02-01

    Specimens of the greasy grouper Epinephelus tauvina (Forsskål), caught off Moorea, French Polynesia, harboured four species of gill monogeneans. The diplectanid Pseudorhabdosynochus pai n. sp. is characterised by an extremely big male quadriloculate organ (inner length 77 mum, cone length 15, tube length 47), the largest of all members of the genus, and a sclerotised vagina with a very complex structure, including three secondary chambers instead of one as in most species. Pseudorhabdosynochus sp. is a species of the 'cupatus group'; this species is not formally described but various measurements are provided. The ancyrocephalid Haliotrema sp. and the capsalid Benedenia sp. were rare; they are both mentioned but not described. The diplectanid fauna of E. tauvina corresponds to the pattern already found in a clade of grouper species, the members of which often harbour both a species of the 'cupatus group' and another species of Pseudorhabdosynochus Yamaguti, 1958.

  20. Two new species of Pseudorhabdosynochus Yamaguti, 1958 (Monogenea: Diplectanidae) from the deep-sea grouper Epinephelus morrhua (Val.) (Perciformes: Serranidae) off New Caledonia.

    PubMed

    Justine, Jean-Lou

    2008-10-01

    Three species of monogeneans were collected from Epinephelus morrhua, a deep-sea grouper from the external slope of the coral reef, off New Caledonia, South Pacific, and are the first parasites recorded from this fish species. Pseudorhabdosynochus morrhua n. sp. is characterised by: a sclerotised vagina with small sclerotised chambers and a short secondary canal; squamodiscs with central rows of rodlets which form closed ovals; and a scaly tegument. P. variabilis n. sp. has: a male quadriloculate organ with a characteristic structure; a sclerotised vagina in which the primary canal, secondary canal and accessory structure are very long and the different parts have various arrangements in different specimens; its squamodiscs have central rows of rodlets which form closed circles; and a smooth tegument. A prominent vaginal structure, comparable to that of P. variabilis, has been found only in P. dolicocolpos Neifar & Euzet, 2007, but the species can be distinguished by details of the vagina and other structures. A species of Haliotrema Johnson & Tiegs, 1922 (Ancyrocephalidae) is reported but not described. Specimens of each of the three species were present in similar numbers.

  1. Two new species of ancyrocephalid monogeneans from Lethrinus rubrioperculatus Sato (Perciformes: Lethrinidae) off New Caledonia, with the proposal of Lethrinitrema n. g.

    PubMed

    Lim, L H S; Justine, Jean-Lou

    2011-02-01

    Lethrinitrema gibbus n. g., n. sp. and L. dossenus n. sp. are described from the fish Lethrinus rubrioperculatus Sato collected off New Caledonia, South Pacific. Members of Lethrinitrema n. g. (Ancyrocephalidae) are characterised by having two pyriform haptoral reservoirs and ventral anchors with lateral grooves. The elongate tubular distal end of each reservoir bifurcates, draining into a superficial lateral groove on each side of the ventral anchors. The haptoral reservoirs are postulated to store secretory products which assist in attachment to the host. Lethrinitrema spp. also possess tandem gonads, a male copulatory organ without an accessory piece or with thinly sclerotised accessory piece, and a dextrolateral, non-sclerotised vaginal bulb. The two new species have small, poorly demarcated haptors with small haptoral armament and a crown-like piece on the tip of the inner root of the ventral anchors. They differ from each other in the shape and size of the ventral bar and male copulatory organ (40-45 μm in length in L. gibbus vs 24-30 μm in L. dossenus). Three other species, previously included in Haliotrema Johnston & Tiegs, 1922, are transferred to Lethrinitrema, i.e. L. chrysostomi (Young, 1968) n. comb., L. fleti (Young, 1968) n. comb. (both briefly redescribed from paratypes) and L. lethrini (Yamaguti, 1937) n. comb. All species of Lethrinitrema parasitise Lethrinus spp. (Lethrinidae), and there is evidence for the existence of further Lethrinitrema spp. on Lethrinus spp. in the Indo-Pacific region.

  2. Growth and mortality of Cichla spp. (Perciformes, Cichlidae) introduced in Volta Grande Reservoir (Grande River) and in a small artificial lake in Southeastern Brazil.

    PubMed

    Gomiero, L M; Carmassi, A L; Rondineli, G R; Villares Junior, G A; Braga, F M S

    2010-11-01

    The growth and mortality parameters were estimated through the analysis of length frequency distribution for species of Cichla spp. introduced into a lake in Leme (SP), and in Volta Grande reservoir (SP-MG). In Leme, Cichla kelberi presented larger frequency in the inferior classes of lengths, larger instantaneous rate of natural mortality, and smaller number of cohorts than C. kelberi and C. piquiti in Volta Grande. The values of growth performance obtained for the species were similar, corroborating the validity of the estimated growth and mortality parameters. The increase of the growth rate in small and less diverse environments occurs due to predation. The genus Cichla adapts well in locations in which it is introduced, however this adaptation shows itself to be strongly adjusted to each particular location, determining great plasticity and establishment capacity.

  3. A new species of Neobathyclupea from the northern Indian Ocean with comments on N. malayana (Teleostei, Perciformes, Bathyclupeidae).

    PubMed

    Prokofiev, Artem M; Gon, Ofer; Psomadakis, Peter N

    2016-12-19

    A new species of Neobathyclupea is described from two specimens collected off Myanmar and off Socotra Island. The new species is most similar to N. malayana, but differs from it and other congeners in having jet-black coloured fins, larger pseudobranch, dorsal-pterygiophore pattern and some body proportions. Neobathyclupea malayana is re-diagnosed. Individual variations in armament of the preopercle and in the dorsal-pterygiophore patterns within Bathyclupeidae are discussed.

  4. [Length, body weight and sex of the golden Coryphaena hippurus (Perciformes: Coryphaenidae), of the littoral of Sinaloa, Nayarit and Baja California Sur, Mexico].

    PubMed

    Madrid, J V; Beltrán-Pimienta, R

    2001-01-01

    Dolphin fish population data were recorded from landings in Sinaloa, Nayarit and Baja Califonia Sur, Mexico, in 1997. Of 3,211 organisms, only 2,812 produced complete biometric data. The maximal length and weight were 192 cm and 30 kg. The three sampling sites and the climatic periods also were associated with different size (P < 0.05). The covariate analysis of weight and length relationships indicated significative differences (P < 0.05) for total data and by sex. Condition factor was higher throughout the study period in Mazatlán, and the variation was higher, in the spring. The sexual ratio was in Mazatlán constant, while there were more males in Nayarit in the fall and the spring and in Los Cabos in the spring. There may be population differences, between the central mexican Pacific Coast and the Peninsula of Baja data.

  5. Description of a new species of Priolepis (Perciformes: Gobiidae) from the Red Sea, a new record of Priolepis compita, and a distributional range extension of Trimma fishelsoni.

    PubMed

    Bogorodsky, Sergey V; Suzuki, Toshiyuki; Mal, Ahmad O

    2016-08-11

    A new species of Reefgoby, Priolepis melanops, is described from Al Lith, central Saudi Arabian coast of the Red Sea, on the basis of a single adult specimen. It is a distinctive species, and can distinguish from its congeners by the following characters: dorsal-fin rays VI + I,9, no elongate spines in first dorsal fin; anal-fin rays I,8; pectoral-fin rays 14-15, all unbranched; longitudinal scale series 25; no scales on the head or predorsal midline, sides of the nape scaled; a developed transverse pattern of the sensory papillae below the eye; fifth pelvic-fin ray unbranched, its length 47% length of fourth ray; body and most of head brownish orange, densely covered with melanophores; snout, lips, chin and chest black; iris black; fins translucent with narrow black stripe along base of each dorsal fin. Placement of the new species in Priolepis is based on the presence of characteristics currently associated with Priolepis rather than with Trimma. An individual of Priolepis compita Winterbottom was photographed in very shallow water on a reef flat at Sharm el Sheikh, at the entrance of the Gulf of Aqaba, and represents a new record for the Red Sea. The endemic Red Sea species Trimma filamentosus Winterbottom and T. fishelsoni Goren, previously know as far south as Jeddah, were collected at Al Lith, central Saudi Arabia, and represents the southernmost record for both species. Variation of P. compita and T. fishelsoni is noted and the cephalic sensory system of the latter is described for the first time. In addition, we report that records of Trimma tevegae Cohen & Davis from the Red Sea are based on misidentification. A key to distinguish the species of Priolepis and Trimma known from the Red Sea is provided.

  6. Infection levels and seasonality of monogeneans in the largemouth bass Micropterus salmoides (Perciformes: Centrarchidae) from Nuevo León, Mexico.

    PubMed

    Galaviz-Silva, L; Iruegas-Buentello, F J; Escobar-González, B; Molina-Garza, Z J

    2016-11-01

    Largemouth bass, Micropterus salmoides, is a native fish species with special importance for sport fishing competitions in Nuevo León, Mexico. However, no study has investigated the parasitic fauna of M. salmoides, and no reports are available on monogenean parasites in this fish species. Therefore, we described the monogenean parasites of M. salmoides and the effects of season and fish condition factor in five reservoirs: La Boca (LB), El Cuchillo-Solidaridad (CS), Sombreretillo (S), Laguna Salinillas (LS) and Cerro Prieto (CP). The monogeneans infecting M. salmoides were Clavunculus unguis and Acolpenteron ureteroecetes (collected in all localities), as well as Syncleithrium fusiformis, Haplocleidus furcatus, Clavunculus bifurcatus and Urocleidus principalis (CS). Clavunculus unguis had the highest prevalence in fish from all reservoirs. The abundance of monogeneans was generally greater in late spring to autumn than in winter. Although season was not correlated with abundance (r s = 0.0934, P <  0.0154), the months of highest temperature (from May to September) were positively correlated with parasite abundance. A significant association was observed between fish condition factor and the presence of monogeneans (P <  0.05), except for A. ureteroecetes. Our findings include five new geographic records for C. unguis, S. fusiformis, H. furcatus and C. bifurcatus.

  7. Mayarhynchus karlae n. g., n. sp. (Acanthocephala: Neoechinorhynchidae), a parasite of cichlids (Perciformes: Cichlidae) in southeastern Mexico, with comments on the paraphyly of Neoechynorhynchus Stiles & Hassall, 1905.

    PubMed

    Pinacho-Pinacho, Carlos D; Hernández-Orts, Jesús S; Sereno-Uribe, Ana L; Pérez-Ponce de León, Gerardo; García-Varela, Martín

    2017-03-01

    Mayarhynchus n. g. (Acanthocephala: Neoechinorhynchidae) is erected for Mayarhynchus karlae n. g, n. sp. described from the intestine of four species of cichlid fishes distributed from southeastern Mexico. The new genus placed in the family Neoechinorhynchidae (Ward, 1917) Van Cleave, 1928, is readily distinguished from the other 17 genera in the family by having a small proboscis armed with 45-46 relatively weak rooted hooks arranged in nine longitudinal rows of five hooks each. In addition, Mayarhynchus n. g., n. sp. is diagnosed by the presence of a short trunk, body wall with five dorsal and one ventral giant hypodermal nuclei, proboscis receptacle nearly cylindrical with single layered wall, lemnisci broad and flat with large nuclei, testes in tandem, cement gland with eight large nuclei, and eggs elongate to oval. Partial sequences of the cytochrome c oxidase subunit 1 (cox1), internal transcribed spacers (ITS1 + 5.8S + ITS2), and the D2-D3 domains of the large subunit rRNA gene (28S) were obtained for five specimens of the new species and other species belonging to the Neoechinorhynchidae. Phylogenetic analyses confirmed that the new genus belongs to the Neoechynorhynchidae and indicated that the genus Neoechynorhynchus Stiles & Hassall, 1905 is not monophyletic. Comparison between three populations of the new species yielded nine variable sites for cox1, 11 for ITS and four for 28S.

  8. A new species of Suezichthys (Teleostei: Perciformes: Labridae) from the south-eastern Pacific, with a redefinition of the genus and a key to species.

    PubMed

    Russell, Barry C; Westneat, Mark W

    2013-01-01

    A new species of the fish family Labridae, Suezichthys rosenblatti, is described from specimens collected at Isla San Felix, Isla Juan Fernandez and Isla San Ambrosio, off the coast of Chile. Suezichthys rosenblatti is distinct in having a combination of 11 dorsal fin soft rays and 11 anal fin soft rays. It falls in the group of species that has 1½ scale rows above the lateral line and lack a scaly sheath at the base of the dorsal and anal fins (S. aylingi Russell, S. caudovittatus Russell, S. gracilis (Steindachner & Döderlein) and S. soelae Russell). Unlike other members of this group, S. rosenblatti has haemal arches on vertebrae 10-11 (versus haemal arch only on vertebra 10). The monotypic Nelabrichthys ornatus (Carmichael) is now included in the genus Suezichthys and a revised generic description and key to species of Suezichthys is provided. The occurrence of S. rosenblatti in the south-eastern Pacific and S. ornatus in the south-western Indian Ocean and south Atlantic Ocean represent major range extensions of the genus Suezichthys.

  9. Morphological and karyotypic differentiation in Caranx lugubris (Perciformes: Carangidae) in the St. Peter and St. Paul Archipelago, mid-Atlantic Ridge

    NASA Astrophysics Data System (ADS)

    Jacobina, Uedson Pereira; Martinez, Pablo Ariel; Cioffi, Marcelo de Bello; Garcia, José; Bertollo, Luiz Antonio Carlos; Molina, Wagner Franco

    2014-03-01

    Isolated oceanic islands constitute interesting model systems for the study of colonization processes, as several climatic and oceanographic phenomena have played an important role in the history of the marine ichthyofauna. The present study describes the presence of two morphotypes of Caranx lugubris, in the St. Peter and St. Paul Archipelago located in the mid-Atlantic. Morphotypes were compared in regard to their morphological and cytogenetic patterns, using C-banding, Ag-NORs, staining with CMA3/DAPI fluorochromes and chromosome mapping by dual-color FISH analysis with 5S rDNA and 18S rDNA probes. We found differences in chromosome patterns and marked divergence in body patterns which suggest that different populations of the Atlantic or other provinces can be found in the Archipelago of St. Peter and St. Paul.

  10. Revision of the circumtropical glasseye fish Heteropriacanthus cruentatus (Perciformes: Priacanthidae), with resurrection of two species.

    PubMed

    Fernandez-Silva, Iria; Ho, Hsuan-Ching

    2017-06-06

    The glasseye fish Heteropriacanthus, previously known as a monotypic genus, is now divided into three species based on morphological and genetic features. After examination on the type specimens and literature, herein we resurrect two junior binomens, H. carolinus (Cuvier, 1829) from the Indo-Pacific Ocean and H. fulgens (Lowe, 1838) from the northeastern Atlantic Ocean. Heteropriacanthus cruentatus (Lacepède, 1801) is now considered to be restricted to the Atlantic and southwestern Indian oceans. In light of these observations we discuss the evolutionary history of the genus.

  11. Investigation into the cytotoxicity and mutagenicity of the Marajó Archipelago waters using Plagioscion squamosissimus (Perciformes: Sciaenidae) as a bioindicator.

    PubMed

    Rocha, Carlos Alberto Machado da; Pessoa, Carla Mariana Ferreira; Rodrigues, Claudia Antonia Campos; Pinheiro, Raul Henrique da Silva; Costa, Edmar Tavares da; Guimarães, Adriana Costa; Burbano, Rommel Rodríguez

    2016-10-01

    Maintaining water quality within tolerable limits is a basic need of the riverside communities in the Amazon. Using endemic aquatic organisms as biological models is useful for monitoring the environment. In this study, potential cytotoxic and genotoxic damages in Plagioscion squamosissimus (commonly known as silver croaker) from the Marajó Archipelago were evaluated using a flow cytometry assay and a survey of micronuclei (MN) frequency as well as other nuclear abnormalities (NA). P. squamosissimus specimens were collected at four locations in the Marajó Archipelago. Blood samples from these fish were used in the flow cytometry assay and piscine micronucleus test, and the resulting data were analyzed using analysis of variance (ANOVA). We did not observe a difference in the erythrocyte cell cycle distribution among the samples (P=0.9992), which suggests the absence of cytotoxic agent-induced apoptosis. The piscine micronucleus test exhibited differences in the samples from São Sebastião da Boa Vista (SSBV), and those from Anajás produced the highest mutagenicity indices. The MN frequencies were low for all groups, but the groups exhibited significantly different frequencies (P=0.0033). Reniform nuclei, nuclei with extensions, and lobed nuclei were combined and considered NA. The frequency differences for these NA were significant among sampling sites (P <0.0001). This report is the first to use flow cytometry in fish to evaluate cytotoxic agent-induced apoptosis. The micronucleus test results indicate the presence of pollutants that can change the genetic material of the fish studied. We also demonstrate that the Amazonian fish P. squamosissimus is important not only as a comestible species but also as an adequate model for biomonitoring in aquatic environments.

  12. Description of three new species of the genus Herichthys (Perciformes: Cichlidae) from eastern Mexico, with redescription of H. labridens, H. steindachneri, and H. pantostictus.

    PubMed

    De La Maza-Benignos, Mauricio; Lozano-Vilano, Ma De Lourdes

    2013-11-06

    Three new species of the genus Herichthys are described on the basis of chromatic, morphometric, and meristic characters. Herichthys pratinus sp. nov. from the Rio Salto in San Luis Potosi is characterized by a steep and shallow predorsal profile, a concavity before the eye, and adult males with a prominent forehead forming a nuchal hump, DXV-XVI, 10-11; and AV, 8-9. Herichthys pame sp. nov. from the Rio Tamasopo in San Luis Potosi is characterized by a gradual and acute predorsal contour, lack of concavity before the eye, and mouth angled slightly downward. Herichthys. molango sp. nov. from Laguna Azteca, Hidalgo is distinguished by small eyes and a slender (slightly broader than long), well-spaced, and indented lower pharyngeal plate with 2 rows of 8-9 medium-sized, lightly pigmented molars flanking the midline. We also provide redescriptions for three species of Herichthys, H. pantostictus, H. labridens, and H. steindachneri and a review of H. bartoni.

  13. Molecular Phylogeny of Weakfish Species of the Stellifer Group (Sciaenidae, Perciformes) of the Western South Atlantic Based on Mitochondrial and Nuclear Data

    PubMed Central

    Barbosa, Andressa Jisely Barreto; Sampaio, Iracilda; Schneider, Horacio; Santos, Simoni

    2014-01-01

    The phylogenetic relationships within the Stellifer group of weakfishes (Stellifer, Odontoscion, Ophioscion, and Bairdiella) were evaluated using 2723 base pairs comprising sequences of nuclear (rhodopsin, TMO-4C4, RAG-1) and mitochondrial (16S rRNA and COI) markers obtained from specimens of nine species. Our results indicate a close relationship between Bairdiella and Odontoscion, and also that the genus Stellifer is not monophyletic, but rather that it consists of two distinct lineages, one clade containing S. microps/S. naso/S. brasiliensis and the other, S. rastrifer/S. stellifer/Stellifer sp. B, which is closer to Ophioscion than the former clade. The O. punctatissimus populations from the northern and southern Brazilian coast were also highly divergent in both nuclear (0.8% for rhodopsin and 0.9% for RAG-1) and mitochondrial sequences (2.2% for 16S rRNA and 7.3% for COI), which we conclude is consistent with the presence of two distinct species. The morphological similarities of the members of the Stellifer group is reinforced by the molecular data from both the present study and previous analyses, which have questioned the taxonomic status of the Stellifer group. If, on the one hand, the group is in fact composed of four genera (Stellifer, Ophioscion, Odontoscion, and Bairdiella), one of the two Stellifer clades should be reclassified as a new genus. However, if the close relationship and the reduced genetic divergence found within the group is confirmed in a more extensive study, including representatives of additional taxa, this, together with the morphological evidence, would support downgrading the whole group to a single genus. Obviously, these contradictory findings reinforce the need for a more systematic taxonomic revision of the Stellifer group as a whole. PMID:25020128

  14. The complete mitochondrial genome of the large yellow croaker, Larimichthys crocea (Perciformes, Sciaenidae): unusual features of its control region and the phylogenetic position of the Sciaenidae.

    PubMed

    Cui, Zhaoxia; Liu, Yuan; Li, Chi Pang; You, Feng; Chu, Ka Hou

    2009-03-01

    To understand the systematic status of Larimichthys crocea in the Percoidei, we determined the complete mitochondrial (mt) genome sequence using 454 sequencing-by-synthesis technology. The complete mt genome is 16,466 bp in length including the typical structure of 22 tRNAs, 2 rRNAs, 13 protein-coding genes and the noncoding control region (CR). Further sequencing for the complete CR was performed using the primers Cyt b-F and 12S-R on six L. crocea individuals and two L. polyactis individuals. Interestingly, all seven CR sequences from L. crocea were identical while the three sequences from L. polyactis were distinct (including one from GenBank). Although the conserved blocks such as TAS and CSB-1, -2, and -3 are readily identifiable in the control regions of the two species, the typical central conserved blocks CSB-D, -E, and -F could not be detected, while they are found in Cynoscion acoupa of Sciaenidae and other Percoidei species. Phylogenetic analysis shows that L. crocea is a relatively recently emerged species in Sciaenidae and this family is closely related to family Pomacanthidae within the Percoidei. L. crocea, as the first species of Sciaenidae with complete mitochondrial genome available, will provide important information on the molecular evolution of the group. Moreover, the genus-specific pair of primers designed in this study for amplifying the complete mt control region will be very useful in studies on the population genetics and conservation biology of Larimichthys.

  15. Unitubulotestis sardae (Trematoda: Didymozoidae) infection in Atlantic bonito Sarda sarda (Perciformes: Scomberomoridae) in the Ionian and Tyrrhenian Seas: histopathological and SEM investigations.

    PubMed

    Marino, F; Giannetto, S; Cavallaro, M; Paradiso, M L; Bottari, T; De Vico, G

    2003-04-01

    Nematobothrinae are trematoda digenea that usually live in cysts in fish tissues. They have been reported in different teleost species although the host-parasite relationships remain to be elucidated. This report is aimed at analysing the presence of Unitubulotestis sardae in the Atlantic bonito (Sarda sarda) populations fished in the Straits of Messina. The histopathological change reported in literature in similar cases is an aneurisma verminosa saccata, whereas, in the specimens here described, we show neovascularisation and at the same time, hyperplasia of the gill epithelium, leading to the formation of a bistratified cystic wall. The SEM examination of the parasitic cysts gave us a better understanding of the surface microanatomical details of the cystic walls. The external surface appeared to be constituted by a pluristratified covering epithelium of the gill arch. The internal layer was constituted by a net of blood vessels richly anastomosed with those originating from the gill arch arteries. This could be in agreement with data reported by Grabda (1947) on the localisation of the juvenile stages of the parasite in this blood vessel, but the evolution in cysts formation does not show similarities with the formation of an aneurisma.

  16. Testing the potential of DNA barcoding in vertebrate radiations: the case of the littoral cichlids (Pisces, Perciformes, Cichlidae) from Lake Tanganyika.

    PubMed

    Breman, Floris C; Loix, Sara; Jordaens, Kurt; Snoeks, Jos; Van Steenberge, Maarten

    2016-11-01

    We obtained 398 cytochrome c oxidase subunit I barcodes of 96 morphospecies of Lake Tanganyika (LT) cichlids from the littoral zone. The potential of DNA barcoding in these fishes was tested using both species identification and species delineation methods. The best match (BM) and best close match (BCM) methods were used to evaluate the overall identification success. For this, three libraries were analysed in which the specimens were categorized into Operational Taxonomic Units (OTU) in three alternative ways: (A) morphologically distinct, including undescribed, species, (B) valid species and (C) complexes of morphologically similar or closely related species. For libraries A, B and C, 73, 73 and 96% (BM) and 72, 70 and 94% (BCM) of the specimens were correctly identified. Additionally, the potential of two species delineation methods was tested. The General Mixed Yule Coalescent (GMYC) analysis suggested 70 hypothetical species, while the Automatic Barcode Gap Discovery (ABGD) method revealed 115 putative species. Although the ABGD method had a tendency to oversplit, it outperformed the GMYC analysis in retrieving the species. In most cases where ABGD suggested oversplitting, this was due to intraspecific geographical variation. The failure of the GMYC method to retrieve many species could be attributed to discrepancies between mitochondrial gene trees and the evolutionary histories of LT cichlid species. Littoral LT cichlids have complex evolutionary histories that include instances of hybridization, introgression and rapid speciation. Nevertheless, although the utility of DNA barcoding in identification is restricted to the level of complexes, it has potential for species discovery in cichlid radiations. © 2016 John Wiley & Sons Ltd.

  17. Parapercis nigrodorsalis (Perciformes: Pinguipedidae), a new species of sandperch from northern New Zealand and the Norfolk Ridge, Tasman Sea and remarks on P. binivirgata (Waite, 1904).

    PubMed

    Johnson, Jeffrey W; Struthers, Carl D; Wilmer, Jessica Worthington

    2014-08-26

    A new species of pinguipedid fish, Parapercis nigrodorsalis, is described from 17 specimens collected off the North Island of New Zealand and Wanganella Bank, Norfolk Ridge, Tasman Sea, in depths of 56-280 m. The species has also been photographed underwater off the Poor Knights Islands Reserve and Burgess Island, Mokohinau Group, in New Zealand. It is most similar to Parapercis binivirgata (Waite, 1904) in morphology, coloration and meristic values, but is unique among the genus in having a combination of dorsal-fin rays V, 23, anal-fin rays I, 19, lateral-line scales 57-63, vomer with 1-2 irregular rows of robust conical teeth, palatines with 1-2 rows of small teeth, angle of subopercle smooth, 10 abdominal and 22 caudal vertebrae, and coloration, including seven broad reddish-brown bands on the upper body between the spinous dorsal-fin and the caudal peduncle, most bands bifurcated into close-set double bars with black smudge-like blotches below, and membrane of the spinous dorsal fin black. Comparison of the mitochondrial cytochrome c oxidase subunit 1 (CO 1) genetic marker utilised in DNA barcoding produced a genetic divergence of 5.38% and 7.63% between the new species and its two closest sampled congeners. The holotype of P. binivirgata is identified from two specimens previously regarded as syntypes, some revisions are made to meristic data in the original description of the latter, and a detailed description of the revised geographic range of P. binivirgata is provided.  

  18. Taxonomical notes on selected freshwater fish species described from northern and central Vietnam (Cypriniformes: Balitoridae, Cobitidae, Cyprinidae, Nemacheilidae; Perciformes: Channidae, Osphronemidae; Synbranchiformes: Mastacembelidae).

    PubMed

    Endruweit, Marco

    2014-03-01

    Selected, little known taxa of northern and central Vietnamese freshwater fish species are reviewed. Nomenclatural acts are taken: Hemibarbus lehoai is placed in synonymy of H. maculatus, Paracobitis hagiangensis in synonymy of Schistura caudofurca. A neotype of Micronemacheilus bacmeensis is assigned. The name Channa hanamensis is treated as a nomen nudum. Two labeonine species described from China are nomenclaturally affected: Garra findolabium is transferred to Vinagarra and its specific epithet is treated as a noun in apposition; the specific epithet of Sinigarra napoense is corrected to napoensis.

  19. Site fidelity, size, and morphology may differ by tidal position for an intertidal fish, Bathygobius cocosensis (Perciformes-Gobiidae), in Eastern Australia

    PubMed Central

    Malard, Lucie A.; McGuigan, Katrina

    2016-01-01

    The intertidal zone is a transitional environment that undergoes daily environmental fluctuations as tides rise and fall. Relatively few fish species are adapted to endure the physiological pressures of this environment. This study focused on Bathygobius cocosensis (Gobiidae), a common intertidal fish in New South Wales, Australia. We investigated whether shore height impacted site fidelity, survival probability, fish size, and morphological traits with respect to tidal height. Mark-recapture methods were used over a five month period to determine if individuals in high shore pools had greater site fidelity; fish in high tide pools were more than twice as likely to be recaptured in their original pool than fish from low tide pools. High pool individuals were, on average, smaller with larger eyes and longer snouts relative to their size as compared to low pool individuals. We discuss several mechanisms that could cause the observed pattern in morphological variation. Ultimately, this study suggests that within species behaviour and morphology differ by tidal position for an intertidal fish. PMID:27547568

  20. Caesio xanthalytos, a new species of fusilier (Perciformes: Caesionidae) from the Western Indian Ocean, with records of range extensions for several species of Caesionidae.

    PubMed

    Holleman, Wouter; Connell, Allan D; Carpenter, Kent E

    2013-01-01

    Caesio xanthalytos, a new species of fusilier, closely related to Caesio caerulaurea Lacepède 1801, is described from several specimens from various localities on the east coast of Africa, and from southern Madagascar. While very similar in colour pattern to C. caerulaurea, its lateral line lies within a dominant, composite yellow stripe, whereas in C. caurulaurea the lateral line lies immediately below the yellow stripe. The two species also differ in the number of lateral-line scales, the modal number of pectoral-fin rays, in the form of the nuchal scale patch, and genetically. The new species has been observed to school with C. caerulaurea and with C. varilineata Carpenter 1987. The ranges of several species of Caesio and Pterocaesio and of Dipterygonotus balteatus and Gymnocaesio gymnoptera are extended to localities on the South African east coast.

  1. Taxonomical notes on selected freshwater fish species described from northern and central Vietnam (Cypriniformes: Balitoridae, Cobitidae, Cyprinidae, Nemacheilidae; Perciformes: Channidae, Osphronemidae; Synbranchiformes: Mastacembelidae)

    PubMed Central

    Endruweit, Marco

    2014-01-01

    Selected, little known taxa of northern and central Vietnamese freshwater fish species are reviewed. Nomenclatural acts are taken: Hemibarbus lehoai is placed in synonymy of H. maculatus, Paracobitis hagiangensis in synonymy of Schistura caudofurca. A neotype of Micronemacheilusbacmeensis is assigned. The name Channa hanamensis is treated as a nomen nudum. Two labeonine species described from China are nomenclaturally affected: Garra findolabium is transferred to Vinagarra and its specific epithet is treated as a noun in apposition; the specific epithet of Sinigarra napoense is corrected to napoensis. PMID:24668657

  2. An Investigation into the Physico-chemical Factors Affecting the Abundance and Diversity of Aquatic Insects in Organically Manured Aquadams and Their Utilization by Oreochromis mossambicus (Perciformes: Cichlidae).

    PubMed

    Rapatsa, M M; Moyo, N A G

    2015-08-01

    The interaction between the fish Oreochromis mossambicus (Percifomes: Cichlidae) and aquatic insects after application of chicken, cow, and pig manure was studied in 7,000-liter plastic aquadams. Principal component analysis showed that most of the variation in water quality after application of manure was accounted for by potassium, nitrogen, dissolved oxygen, phosphate, and alkalinity. Canonical correspondence analysis showed that Gyrinidae, Elminidae, Hydrophilidae, Hydraenidae, and Athericidae were associated with high nutrient levels (nitrogen, phosphorus, and potassium) characteristic of the chicken manure. However, the most abundant aquatic insects Gerridae, Notonectidae, and Culicidae were close to the centre of the ordination and not defined by any nutrient gradient. The Shannon-Wiener diversity was highest in the aquadams treated with chicken manure. The most frequently occurring aquatic insects in the diet of O. mossambicus were culicid mosquitoes in all the treatments. However, in the laboratory, Chironomidae were the most preferred because they lacked refuge. Notonectidae and Gerridae were not recorded in the diet of O. mossambicus despite their abundance. This may be because of their anti-predation strategies. Laboratory experiments showed that Notonectidae, Gyrinidae, and Gerridae fed on Chironomidae and Culicidae. This implies that aquatic predatory insects competed for food with O. mossambicus. © The Authors 2015. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com.

  3. Functional Categorization of Transcriptome in the Species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) Exposed to Benzo[a]pyrene and Phenanthrene

    PubMed Central

    Lemgruber, Renato de Souza Pinto; Marshall, Nislanha Ana dos Anjos; Ghelfi, Andrea; Fagundes, Daniel Barros; Val, Adalberto Luis

    2013-01-01

    This study aims to evaluate the transcriptome alterations, through cDNA libraries, associated with the combined effects of two PAHs, benzo[a]pyrene (0.5 µg/L) and phenanthrene (50 µg/L), present in crude oil, on specimens of Symphysodon aequifasciatus (discus fish) after 48 h of exposure. The cDNA libraries were constructed according to the SOLiD™ SAGE™ protocol for sequencing in the SOLiD v.3 Plus sequencer. The results were analyzed by bioinformatics and differentially expressed genes were categorized using the gene ontology program. The functional categories (terms) found in the gene ontology and the gene network generated using STRING software were used to predict the adverse effects of benzo[a]pyrene and phenanthrene in the liver. In the present study, 27,127 genes (compared to Danio rerio database) were identified. Considering only those genes with a p-value less than or equal to 0.05 and greater than or equal to two-fold change in expression across libraries, we found 804 genes, 438 down-regulated (54%) and 366 up-regulated (46%), in the experimental group compared to the control. Out of this total, 327 genes were successfully categorized, 174 down-regulated and 153 up-regulated, using gene ontology. Using String, the gene network was composed by 199 nodes, 124 of them resulting in 274 interactions. The results showed that even an acute exposure of 48 h caused metabolic change in response to environmental contaminants, resulting in changes of cell integrity, in oxidation-reduction processes, in the immune response and disturbances of intracellular signaling of discus fish. Also the gene network has showed no central interplay cluster, exhibiting instead interconnected clusters interactions and connected sub-networks. These findings highlight that even an acute sublethal exposure of PAHs can cause metabolism changes that may affect survival of discus. Our findings using SOLiD coupled with SAGE-method resulted in a powerful and reliable means for gene expression analysis in discus, a non-model Amazonian fish. PMID:24312524

  4. The EcoRI centromeric satellite DNA of the Sparidae family (Pisces, Perciformes) contains a sequence motive common to other vertebrate centromeric satellite DNAs.

    PubMed

    Garrido-Ramos, M A; Jamilena, M; Lozano, R; Ruiz Rejón, C; Ruiz Rejón, M

    1995-01-01

    By means of cloning, sequencing, and fluorescence in situ hybridization, we have determined that the EcoRI satellite DNA family is conserved in the 10 sparid species analyzed here. Its conservation, its chromosomal location at the centromere of each chromosome, and its structural features could make this satellite DNA family an important structural and/or functional element of the centromeres of these species. Monomeric units of this satellite DNA have a consensus length of 187 bp. Its sequence is characterized by a high AT content and the presence of short runs of consecutive AT base pairs. These monomeric EcoRI repeats also contain three to four copies, depending on the species, of a short sequence reflecting the repetitive duplication and subsequent divergence of an ancestral 9-bp sequence in this family. This sequence motive is conserved in some parts of the monomeric units of the different species studied at the same positions, and, precisely, surrounding the area in which the curvature of the monomeric molecule is greatest. The 9-bp sequence motive is similar to other direct-repeat sequences of the centromeric satellite DNAs of other vertebrates, including those of amphibians and mammals.

  5. Mulloidichthys flavolineatus flavicaudus Fernandez-Silva & Randall (Perciformes, Mullidae), a new subspecies of goatfish from the Red Sea and Arabian Sea

    PubMed Central

    Fernandez-Silva, Iria; Randall, John E.; Golani, Daniel; Bogorodsky, Sergey V.

    2016-01-01

    Abstract The number of goatfish species has increased recently, thanks in part to the application of molecular approaches to the taxonomy of a family with conservative morphology and widespread intraspecific color variation. A new subspecies Mulloidichthys flavolineatus flavicaudus Fernandez-Silva & Randall is described from the Red Sea and Arabian Sea, including Socotra and Gulf of Oman. It is characterized by a yellow caudal fin, 25–28 gill rakers, and 37–38 lateral-line scales and it is differentiated from nominal subspecies Mulloidichthys flavolineatus flavolineatus by 1.7% sequence divergence at the mitochondrial cytochrome b gene. The morphometric examination of specimens of Mulloidichthys flavolineatus flavolineatus revealed variation in head length, eye diameter, and barbel length, in western direction from the Hawaiian Islands, South Pacific, Micronesia, and the East Indies to the Indian Ocean. The population of Mulloidichthys flavolineatus flavicaudus subsp. n. in the Gulf of Aqaba differs from that of the remaining Red Sea by shorter barbels, smaller eyes, shorter head, and shorter pelvic fins. We present a list of 26 endemic fishes from the Gulf of Aqaba and discuss the probable basis for the endemism in the light of the geological history of this region. PMID:27551217

  6. Homogeneous Nature of Malaysian Marine Fish Epinephelus fuscoguttatus (Perciformes; Serranidae): Evidence Based on Molecular Markers, Morphology and Fourier Transform Infrared Analysis

    PubMed Central

    Nurdalila, A’wani Aziz; Bunawan, Hamidun; Kumar, Subbiah Vijay; Rodrigues, Kenneth Francis; Baharum, Syarul Nataqain

    2015-01-01

    Taxonomic confusion exists within the genus Epinephelus due to the lack of morphological specializations and the overwhelming number of species reported in several studies. The homogenous nature of the morphology has created confusion in the Malaysian Marine fish species Epinephelus fuscoguttatus and Epinephelus hexagonatus. In this study, the partial DNA sequence of the 16S gene and mitochondrial nucleotide sequences of two gene regions, Cytochrome Oxidase Subunit I and III were used to investigate the phylogenetic relationship between them. In the phylogenetic trees, E. fuscoguttatus was monophyletic with E. hexagonatus species and morphology examination shows that no significant differences were found in the morphometric features between these two taxa. This suggests that E. fuscoguttatus is not distinguishable from E. hexagonatus species, and that E. fuscoguttatus have been identified to be E. hexagonatus species is likely attributed to differences in environment and ability to camouflage themselves under certain conditions. Interestingly, this finding was also supported by Principal Component Analysis on Attenuated Total Reflectance–Fourier-transform Infrared (ATR-FTIR) data analysis. Molecular, morphological and meristic characteristics were combined with ATR-FTIR analysis used in this study offer new perspectives in fish species identification. To our knowledge, this is the first report of an extensive genetic population study of E. fuscoguttatus in Malaysia and this understanding will play an important role in informing genetic stock-specific strategies for the management and conservation of this highly valued fish. PMID:26147421

  7. [Phosphoethanolamine in the brain of the eurythermal pond fish Perccottus glehni (Eleotridae, Perciformes, Dyb. 1877) as a phenomenon depending on temperature factor].

    PubMed

    Karanova, M V

    2013-01-01

    Effects of a seasonal decrease of environmental temperature and cold shock on pools of free amino acids (FAA) was studied in brain of the eurythermal pond fish P. glehni. It is for the first time that in the brain of eurythermal animals the nonprotein amino acid phosphoethanolamine (PEA) was revealed. It was found there in a large amount only under conditions of near-zero temperatures. It was shown that in the P. glehni brain, as a result of the seasonal decrease of temperature, the PEA pool rose intensively from 0.3 % at the summer period to 33.6 % in winter. On the contrary, the brain taurine pool that in the summer was the highest as compared with other FAA (29.0% of the total pool) decreased to 8.9% by the beginning of the winter period. The same negative correlation of the taurine and PEA amounts was found under action of acute cold shock (+1 degree C): throughout 4 days the taurine level also decreased from 32.2 to 14.5% of the total pool, whereas the PEA level rose swiftly (from 2.1 to 15.3%). Both kinds of the low temperature action led, apart from PEA, to an intensive increase of the serine pool and accumulation of phosphoserine. Role of PEA in biochemical evolution and adaptation of brain to low temperatures is discussed. It is suggested that accumulation of PEA, phosphoserine, and serine is associated with a change of the status of phospholipids of membrane at low temperatures.

  8. Transcriptome of the Deep-Sea Black Scabbardfish, Aphanopus carbo (Perciformes: Trichiuridae): Tissue-Specific Expression Patterns and Candidate Genes Associated to Depth Adaptation

    PubMed Central

    Stefanni, Sergio; Bettencourt, Raul; Pinheiro, Miguel; Moro, Gianluca De; Bongiorni, Lucia; Pallavicini, Alberto

    2014-01-01

    Deep-sea fishes provide a unique opportunity to study the physiology and evolutionary adaptation to extreme environments. We carried out a high throughput sequencing analysis on a 454 GS-FLX titanium plate using unnormalized cDNA libraries from six tissues of A. carbo. Assemblage and annotations were performed by Newbler and InterPro/Pfam analyses, respectively. The assembly of 544,491 high quality reads provided 8,319 contigs, 55.6% of which retrieved blast hits against the NCBI nonredundant database or were annotated with ESTscan. Comparison of functional genes at both the protein sequences and protein stability levels, associated with adaptations to depth, revealed similarities between A. carbo and other bathypelagic fishes. A selection of putative genes was standardized to evaluate the correlation between number of contigs and their normalized expression, as determined by qPCR amplification. The screening of the libraries contributed to the identification of new EST simple-sequence repeats (SSRs) and to the design of primer pairs suitable for population genetic studies as well as for tagging and mapping of genes. The characterization of the deep-sea fish A. carbo first transcriptome is expected to provide abundant resources for genetic, evolutionary, and ecological studies of this species and the basis for further investigation of depth-related adaptation processes in fishes. PMID:25309900

  9. Site fidelity, size, and morphology may differ by tidal position for an intertidal fish, Bathygobius cocosensis (Perciformes-Gobiidae), in Eastern Australia.

    PubMed

    Malard, Lucie A; McGuigan, Katrina; Riginos, Cynthia

    2016-01-01

    The intertidal zone is a transitional environment that undergoes daily environmental fluctuations as tides rise and fall. Relatively few fish species are adapted to endure the physiological pressures of this environment. This study focused on Bathygobius cocosensis (Gobiidae), a common intertidal fish in New South Wales, Australia. We investigated whether shore height impacted site fidelity, survival probability, fish size, and morphological traits with respect to tidal height. Mark-recapture methods were used over a five month period to determine if individuals in high shore pools had greater site fidelity; fish in high tide pools were more than twice as likely to be recaptured in their original pool than fish from low tide pools. High pool individuals were, on average, smaller with larger eyes and longer snouts relative to their size as compared to low pool individuals. We discuss several mechanisms that could cause the observed pattern in morphological variation. Ultimately, this study suggests that within species behaviour and morphology differ by tidal position for an intertidal fish.

  10. Colobomatus kimi sp. nov. (Copepoda: Philichthyidae) parasitic in the dwarf goatfish Upeneus parvus Poey, 1852 (Perciformes: Mullidae) in the South Atlantic Ocean.

    PubMed

    Paschoal, Fabiano; Pereira, Aldenice N; Luque, José Luis

    2016-10-11

    A new species of copepod, Colobomatus kimi sp. nov., belonging to the cyclopoid family Philichthyidae Vogt, 1877, is proposed based on female specimens collected from the pores of the cephalic sensory system of the dwarf goatfish, Upeneus parvus Poey from the southeastern Brazilian coastal zone. The new species can be distinguished from its closest congeners by the unique combination of characters displayed by the female, including the forked caudal rami, the position of the midventral cephalic process shorter in relation to the lateral cephalic processes, and the presence of paired genital processes. The new species is the first member of Colobomatus Hesse, 1873 found to parasitize mullids of the genus Upeneus.

  11. Complete mitochondrial genome of the red drum, Sciaenops ocellatus (Perciformes, Sciaenidae): absence of the typical conserved motif in the origin of the light-strand replication.

    PubMed

    Cheng, Yuanzhi; Shi, Ge; Xu, Tianjun; Li, Haiyan; Sun, Yueyan; Wang, Rixin

    2012-04-01

    In this study, the complete mitochondrial genome of the red drum Sciaenops ocellatus was determined first. The genome was 16,500 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 2 main non-coding regions (the control region and the origin of the light-strand replication); the gene composition and order of which were similar to most other vertebrates. The overall base composition of the heavy strand was T 25.5%, C 30.7%, A 27.5%, and G 16.3%, with a slight AT bias of 53%. Within the control region, the discrete and conserved sequence blocks were identified. Motif 5'-ACCGG-3' rather than 5'-GCCGG-3' was detected in the origin of light-strand replication (O(L)) of red drum, which is rare in the mitogenomes of Sciaenidae species. These results would play an important role in elucidating sequence-function relationships of the O(L).

  12. Does morphology predict trophic niche differentiation? Relationship between feeding habits and body shape in four co-occurring juvenile species (Pisces: Perciformes, Sparidae)

    NASA Astrophysics Data System (ADS)

    Ventura, Daniele; Bonhomme, Vincent; Colangelo, Paolo; Bonifazi, Andrea; Jona Lasinio, Giovanna; Ardizzone, Giandomenico

    2017-05-01

    Feeding habits, diet overlap and morphological correlates of four juvenile species of the genus Diplodus were investigated during their settlement periods, along the Tyrrhenian coast. Stomach content analysis showed that the diets of D. sargus and D. puntazzo mainly comprised benthic prey such as harpacticoid copepods, amphipods and polychaetes. On the other hand, D. vulgaris and D. annularis fed mainly on planktonic prey such as ciclopoids, calanoids copepods and fish larvae. A biologically significant diet overlap, calculated using the Schoener index, was recorded between D. sargus and D. puntazzo and between D. vulgaris and D. annularis. Morphological characters related to feeding such as gape height and gut length with their relative growth patterns suggested that different trophic preferences have led to a morphological diversification of feeding structures. Therefore, a geometric morphometric outline method, namely Elliptic Fourier Analysis (EFA) was used to examine shape modification of the head and body regions. The multivariate analyses performed on shape descriptors demonstrated that the four species were morphologically distinct due to different feeding habits: the two species which feed mainly on benthic prey presented a discoidal shape, with broad profiles and rounded head; by contrast, the other two species which relied mostly on planktonic prey, presented a streamlined and more elongated body shape.

  13. Two new species of Cichlidogyrus (Monogenea: Dactylogyridae) parasitizing the gills of African cichlid fishes (Perciformes) from Senegal: morphometric and molecular characterization.

    PubMed

    Rehulková, Eva; Mendlová, Monika; Simková, Andrea

    2013-04-01

    Two new species of Cichlidogyrus are described from the gills of three species of African cichlids collected from the Gambia River basin in the Niokolo-Koba National Park, Senegal: Cichlidogyrus dracolemma n. sp. from Hemichromis letourneuxi and Cichlidogyrus nageus n. sp. from Sarotherodon galilaeus and Tilapia guineensis. The new species are recognized on the basis of morphological differences in the male copulatory organs (MCOs) and apparent differences in the SSU rDNA and ITS1 sequence data. C. dracolemma n. sp. resembles specimens of Cichlidogyrus from Hemichromis bimaculatus, probably misidentified as Cichlidogyrus bychowskii by Paperna in 1965, in having morphologically similar types of haptoral sclerites and MCO. The new species is characterized by possessing an MCO with a kite-shaped termination of the accessory piece and a vagina distally surrounded by a circular superficial sclerotization of the body surface. Molecular phylogenetic analyses showed a strongly supported clade including C. dracolemma n. sp. and two species collected from Hemichromis fasciatus, namely Cichlidogyrus longicirrus Paperna, 1965 and Cichlidogyrus falcifer Dossou and Birgi, 1984. Based on both morphology and molecular phylogeny, C. nageus n. sp. appears to be closely related to Cichlidogyrus acerbus Dossou, 1982 obtained from S. galilaeus. Morphologically, C. nageus n. sp. is readily separated from all known congeners by the characteristic pestle shaped termination of the accessory piece of the MCO. Molecular phylogenetic reconstruction showed that C. dracolemma n. sp. and C. nageus n. sp. cluster with the Cichlidogyrus species possessing the same haptoral configuration and host preferences (on subfamily and tribe level, respectively).

  14. Genetic relationships of Plagioscion squamosissimus (Perciformes, Sciaenidae) from five Neotropical river basins evaluated using mitochondrial atpase6/8 gene sequences.

    PubMed

    Diamante, N A; Prioli, S M A P; Oliveira, A V; Fabrin, T M C; Prioli, L M; Prioli, A J

    2017-07-01

    The genetic relationships of native or introduced Plagioscion squamosissimus in five Brazilian Neotropical basins were evaluated using the mitochondrial atpase6/8 genes. Results revealed that the population of the Tocantins River basin is more basal than the native populations of the Amazon and Parnaíba River basins. Moreover, the populations of P. squamosissimus that were introduced in the São Francisco and upper Paraná River basins originated from the population of the Parnaíba River. © 2017 The Fisheries Society of the British Isles.

  15. Studies on the alterations in haematological indices, micronuclei induction and pathological marker enzyme activities in Channa punctatus (spotted snakehead) perciformes, channidae exposed to thermal power plant effluent.

    PubMed

    Javed, Mehjbeen; Ahmad, Irshad; Ahmad, Ajaz; Usmani, Nazura; Ahmad, Masood

    2016-01-01

    The present study was conducted to assess the toxicity of thermal power plant effluent containing heavy metals (Fe > Cu > Zn > Mn > Ni > Co > Cr) on haematological indices, micronuclei, lobed nuclei and activity of pathological marker enzymes [alkaline phosphatase (ALP), aspartate transferase (AST), alanine transferase (ALT) and creatine kinase (CK)] in Channa punctatus. Total erythrocyte count (-54.52 %), hemoglobin (-36.98 %), packed cell volume (-36.25 %), mean corpuscular hemoglobin concentration (-1.41 %) and oxygen (O2) carrying capacity (-37.04 %) declined significantly over reference fish, however total leukocyte count (+25.43 %), mean corpuscular hemoglobin (+33.52 %) and mean corpuscular volume (+35.49 %) showed elevation. High frequency of micronuclei (1133.3 %) and lobed nuclei (150 %) were observed in exposed fish which may indicate mutagenesis. Activities of pathological marker enzymes ALP, AST, ALT and CK increased significantly in serum of exposed fish. The ratio of ALT: AST in exposed fish was beyond 1 which indicates manifestation of pathological processes. These biomarkers show that fish have macrocytic hypochromic anemia. Leukocytosis showed general defence response against heavy metal toxicity and marker enzymes showed tissue degeneration. In conclusion, thermal power plant effluent has strong potential to induce micronuclei, tissue pathology, making the fish anemic, weak, stressed and vulnerable to diseases.

  16. Phylogeny and taxonomy of sculpins, sandfishes, and snailfishes (Perciformes: Cottoidei) with comments on the phylogenetic significance of their early-life-history specializations.

    PubMed

    Smith, W Leo; Busby, Morgan S

    2014-10-01

    Despite recent progress on the higher-level relationships of the Cottoidei and its familial components, phylogenetic conflict and uncertainty remain within the Cottoidea. We analyzed a dataset composed of 4518 molecular (mitochondrial 12S, tRNA-Val, 16S, and cytochrome b and nuclear TMO-4c4, Histone H3, and 28S) and 72 morphological characters for 69 terminals to address cottoid intrarelationships. The resulting well-resolved phylogeny was used to produce a revised taxonomy that is consistent with the available molecular and morphological data and recognizes six families: Agonidae, Cottidae, Jordaniidae, Psychrolutidae, Rhamphocottidae, and Scorpaenichthyidae. The traditional Agonidae was expanded to include traditional hemitripterids and Hemilepidotus. The traditional Cottidae was restricted to Leptocottus, Trachidermus, and the riverine, lacustrine, and Lake Baikal freshwater cottoids. Jordaniidae (Jordania and Paricelinus) was separated from the traditional cottids; Psychrolutidae was expanded from the traditional grouping to include nearly all traditional marine cottids and the single species of bathylutichthyid. Rhamphocottidae was expanded to include the traditional ereuniids, and Scorpaenichthyidae separated Scorpaenichthys from the traditional cottids. The importance of early-life-history characters to the resulting phylogeny and taxonomy were highlighted. Copyright © 2014 Elsevier Inc. All rights reserved.

  17. Kudoa azevedoi n. sp. (Myxozoa, Multivalvulida) from the oocytes of the Atlantic horse mackerel Trachurus trachurus (Perciformes, Carangidae) in Tunisian coasts.

    PubMed

    Mansour, Lamjed; Thabet, Aouatef; Chourabi, Kalthoum; Harrath, Abdul Halim; Gtari, Mahr; Al Omar, Suliman Y; Ben Hassine, Oum Kalthoum

    2013-04-01

    A new species Kudoa azevedoi sp. n. (Myxozoa, Multivalvulida) is described in Trachurus trachurus Linnaeus, 1758 (Carangidae) from fishing harbors in Tunisian coasts using spore morphology and SSU rDNA sequence data. The parasite occurs only in ovaries within oocytes of mature and immature specimens. Spores are quadrate in shape in apical view with rounded edges, having four shell valves and four symmetrical polar capsules. They are of small sizes and measure 3.5±0.41 (3-4.2)×4.5±0.44 (4-5.2) length by width. The polar capsules are pyriform in shape measuring 1.5±0.22 (1.5-2)×0.75±0.14 (0.5-1) μm. Infected oocytes are hypertrophied, whitish colored, and filled with mature spores. Plasmodia are tubular and ramified from the inner membrane toward the center of the oocyte. Phylogenetic analysis based on small subunit ribosomal DNA sequences shows the highest similarity (96%) with the ovary parasite Kudoa ovivora. Some morphological details and spore dimensions support the creation of a new species in the genus Kudoa. Mean prevalence among examined females is of about 55.5%. It varies between localities and length of fish. The present myxosporea is the second Kudoa species reported in fish ovaries.

  18. Structural and molecular characterization of Kudoa quraishii n. sp. from the trunk muscle of the Indian mackerel Rastrelliger kanagurta (Perciforme, Scombridae) in Saudi Arabia coasts.

    PubMed

    Mansour, Lamjed; Harrath, Abdel Halim; Abd-Elkader, Omar H; Alwasel, Saleh; Abdel-Baki, Abdel-Azeem S; Al Omar, Suliman Y

    2014-04-01

    A new Myxozoa, Kudoa quraishii n. sp., is reported in the striated muscle of the Indian mackerel Rastrelliger kanagurta from the Red Sea and the Arabian Gulf in Saudi Arabia. Mean prevalence of infection is about 20% and varies between localities. The parasite develops whitish and oval or rounded pseudocysts of 0.2-3 mm in the striated muscles of the body. Pseudocysts are filled with mature spores. Myxospores are quadrate in shape in apical view with rounded edges and ovoid in side view. Each spore is formed by four equal shell valves and four symmetrical polar capsules. Polar capsules are pyriform in apical view and drop-like in side view. Myxospore measurements in micrometers are 6.14 (5.9-6.34) in width, 5.48 (5.3-5.71) in thickness, and 4.27 (4.1-4.42) in length. Polar capsule measurements in apical view in micrometers are 2.08 (1.88-2.28) and 1.31 (1.10-1.52) length by width. Molecular analysis based on SSU rDNA gene shows closest association with K. amamiensis and K. kenti with respectively 98 and 97.2% of similarities.

  19. REPRODUCTIVE PHYSIOLOGY OF YELLOW PERCH (PERCA FLAVESCENS): ENVIRONMENTAL AND ENDOCRINOLOGICAL CUES

    EPA Science Inventory

    An understanding of the mechanisms involved in the regulation of reproductive processes in yellow perch is fundamental for intensive culture of this commercially important, freshwater, perciform fish. This paper describes the annual reproductive cycle of female and male perch. It...

  20. Description of a new species of Trimma (Perciformes: Gobiidae) from the Red Sea, with a discussion of the generic separation of Trimma and Priolepis, with discussion of sensory papillae terminology.

    PubMed

    Hoese, Douglass F; Bogorodsky, Sergey V; Mal, Ahmad O

    2015-10-06

    A new species of Pygmygoby, Trimma quadrimaculatum, n. sp., is described from the Saudi Arabian coast of the Red Sea on the basis of 16 adult specimens. It is small-sized species, with the largest examined specimen reaching only 16.8 mm SL. The new species has characteristics of both Priolepis and Trimma, and easily may be confused with Priolepis randalli and Trimma mendelssohni; it can distinguish from other species by combination of characters: dorsal-fin rays D VI + I,8; anal-fin rays I,7; longitudinal scale series 23-25; median predorsal scales 6-8 (usually 7 or 8); head naked; a pair of modified elongate papillae on nape; fifth pelvic-fin ray with 2 dichotomous branches; body with broad, irregular, brown bars, last bar posteriorly on caudal peduncle extending basally on to rays of caudal fin; head with three dark brown bars below eye; four subcutaneous dark brown spots ventrally on posterior half of body; and basal quarter of second and third membranes of first dorsal fin with diffuse dark blotch. Placement of the new species in Trimma is based on the presence of more characteristics currently associated with Trimma than with Priolepis. The generic separation of the two genera is discussed, suggesting that further work is needed to clarify the separation of these two genera.

  1. Badis laspiophilus, a new miniature addition to the ichthyofauna of West Bengal, north-eastern India, with observations on its ecology and preliminary notes on its ethology (Actinopterygii: Perciformes: Badidae).

    PubMed

    Valdesalici, Stefano; Van Der Voort, Stefan

    2015-07-16

    Badis laspiophilus is described from the Torsa River drainage, West Bengal, India. It can be distinguished from congeners by a combination of characters which include a small size (15.7-21.0 mm SL), 14-16 circumpeduncular row scales, interorbital width 6.7-8.9% SL, upper and lower jaws 8.2-9.2 and 10.2-13.4% SL, respectively, presence of two dorsal-fin blotches and a single round blotch on the anal fin, and absence of cleithral, opercle and dorsolateral caudal peduncle blotches. Its benthic ecology is discussed and the Badis singenensis species group is diagnosed, of which B. laspiophilus and B. singenensis are considered members.

  2. Monogenoideans (Platyhelminthes) from the gill lamellae of the spotted sea trout, Cynoscion nebulosus (Perciformes, Sciaenidae), from the western coast of the Yucatan Peninsula, Mexico, with redescription of Diplectanum bilobatus Hargis 1955 (Diplectanidae).

    PubMed

    Franco, Edgar F Mendoza; Rodríguez, R E del Rio; Tun, M del C Rosado

    2013-08-01

    The gill lamellae ectoparasites of the spotted sea trout, Cynoscion nebulosus (Sciaenidae), in the western coast of the Yucatán Peninsula, Mexico, revealed three species of Monogenoidea: Cynoscionicola heteracantha (Manter 1938) Price, 1962 (Microcotylidae); Choricotyle cynoscioni (MacCallum, 1917) Llewellyn, 1941 (Diclidophoridae); and Diplectanum bilobatus Hargis, 1955 (Diplectanidae). Brief comments about the current taxonomic status as well as supplemental observations of all these monogenoids originally described and/or reported from the same host fish species found in the USA are provided. New illustrations, prevalence and mean intensity of infection, as well morphological and biometric data based on new specimens are shown. C. heteracantha and C. cynoscioni collected in this study represent the second and first records of the species of these genera for the Atlantic coast of Mexico. The specimens of D. bilobatus are provisionally retained within Diplectanum until an emendation of the genus and a formal revision of all named species of this monogenoidean genus are undertaken.

  3. Pseudorhabdosynochus argus n. sp. (Monogenea: Diplectanidae) from Cephalopholis argus, P. minutus n. sp. and Diplectanum nanus n. sp. from C. sonnerati and other monogeneans from Cephalopholis spp. (Perciformes: Serranidae) off Australia and New Caledonia.

    PubMed

    Justine, Jean-Lou

    2007-11-01

    Monogeneans from three species of Cephalopholis, namely C. argus, C. sonnerati and C. boenak, are described from fish caught off New Caledonia, South Pacific, with comparisons with material from off Queensland, Australia. Pseudorhabdosynochus argus n. sp. from C. argus is present off New Caledonia and Australia; it is characterised by its male quadriloculate organ with very elongate cone, and its sclerotised vagina with anterior trumpet, coiled primary canal and distal part with two chambers and an accessory part. C. boenak has no monogeneans off New Caledonia, but off Australia it harbours Pseudorhabdosynochus sp., a new species which is morphologically related to P. argus. P. minutus n. sp. from C. sonnerati is characterised by its minute body and a sclerotised vagina with two spherical chambers. Diplectanum nanus n. sp. from C. sonnerati is characterised by its very small funnel-shaped male copulatory organ and minute body. A new species, Haliotrema sp. from C. sonnerati is characterised by a very elongate tubular penis; it is distinct from H. cromileptis Young, 1968 (redescribed herein from specimens collected from Cromileptes altivelis off New Caledonia). The species described here include the first members of Pseudorhabdosynochus and the first diplectanids described from species of Cephalopholis. There is no evidence for a clade of Pseudorhabdosynochus species specific to members of Cephalopholis, since the species described here share similarities with other species from Epinephelus. However, it is suggested that the gill structure of Cephalopholis spp. imposes selection toward small body sizes for monogeneans.

  4. Parasite biodiversity in a coral reef fish: twelve species of monogeneans on the gills of the grouper Epinephelus maculatus (Perciformes: Serranidae) off New Caledonia, with a description of eight new species of Pseudorhabdosynochus (Monogenea: Diplectanidae).

    PubMed

    Justine, Jean-Lou

    2007-02-01

    Coral reefs are known for their high level of biodiversity, but parasite biodiversity has not been evaluated. Cases such as Epinephelus maculatus, described here, show that the numerical estimation of parasite biodiversity in coral reefs could reach more than ten times the number of fish species; consequently, the extinction of certain fish species from endangered coral reefs would result in the co-extinction of at least ten times the number of parasite species. E. maculatus is a grouper of intermediate size (1-2 kg) and common in the coral reefs of New Caledonia, South Pacific. Based on the examination of more than 800 monogenean specimens, 12 species of monogeneans (ten diplectanids and two ancyrocephalids) were differentiated on the gills. These species of diplectanids have not been found in other epinephelines in the same area and thus are considered as specific to this host. In addition, three species of copepods, and isopod larvae, are present on the gills; E. maculatus thus has a total of 16 species of gill ectoparasites, which can be found together on a single individual fish. Diplectanids include Laticola dae Journo & Justine, 2006, which is the most abundant species representing about 50% of the specimens, and nine species which are rare, each representing 2-7% of the specimens: Diplectanum uitoe n. sp. and eight species of Pseudorhabdosynochus Yamaguti, 1958. D. uitoe, provisionally attributed to Diplectanum Diesing, 1858, is characterised by a small conical penis with internal walls. Pseudorhabdosynochus auitoe n. sp., P. buitoe n. sp., P. cuitoe n. sp., P. duitoe n. sp., P. euitoe n. sp. and P. fuitoe n. sp. are differentiated on the basis of the morphology of the sclerotised vagina, but are very similar in other characteristics; P. guitoe n. sp. is characterised by a quadriloculate organ with very thick walls and a very small sclerotised vagina; and P. huitoe n. sp. is characterised by its sclerotised vagina and by very long ventral and dorsal haptoral bars. Two rare (2-3% of specimens) ancyrocephalids, Haliotrema epinepheli Young, 1969 and Haliotrema sp., are briefly described in relation to the male copulatory organs and haptoral bars; H. epinepheli is apparently a generalist species found in various epinephelines and other fish species. A table of the 50 species of diplectanids (Pseudorhabdosynochus, Laticola Yang et al., 2006, Echinoplectanum Justine & Euzet, 2006 and Diplectanum) from serranids is provided.

  5. Diplectanids (Monogenea) parasitic on the gills of the coralgroupers Plectropomus laevis and P. leopardus (Perciformes, Serranidae) off New Caledonia, with the description of five new species and the erection of Echinoplectanum n. g.

    PubMed

    Justine, Jean-Lou; Euzet, Louis

    2006-07-01

    Echinoplectanum n. g. is erected for diplectanids which have a male copulatory organ comprising a tubular sclerotised penis with a muscular reservoir at its proximal extremity and an protrusible cirrus, often with spiny ridges, at its distal extremity, and a female copulatory organ comprising a sclerotised vaginal sac, often with two thin tubes. All species have similar squamodiscs made of rows of rodlets, with the central rows forming closed circles, and haptoral parts with a similar shape but different measurements; they are distinguished on the basis of the size and morphology of the male copulatory organ and sclerotised vagina. Five new species are included in Echinoplectanum and are all parasites of coralgroupers, Plectropomus spp., off New Caledonia, South Pacific. Two are from P. laevis (Lacépède): E. laeve n. sp. (type-species) has a large elongate penis, 53[Formula: see text]m in length, a cirrus with spiny ridges and a spherical vagina with two long thin tubes; and E. chauvetorum n. sp. has a large elongate penis, 51[Formula: see text]m in length, a cirrus with thin spiny ridges, and a pear-shaped vagina with two short thin tubes. Three species are from P. leopardus (Lacépède): E. leopardi n. sp. has an elongate penis, 36[Formula: see text]m in length, an unspiny cirrus and a triangular vagina; E. pudicum n. sp. has a very small elongate penis 14[Formula: see text]m in length and no visible vagina; and E. rarum n. sp. has a short thick penis 18[Formula: see text]m in length and a ring-shaped vagina with two thin tubes. In addition, Diplectanum plectropomi Young, 1969, from P. maculatus off Western Australia, and D. echinophallus Euzet & Oliver, 1965 from Epinephelus marginatus in the Mediterranean Sea and Senegal, West Africa, both herein redescribed from the type-specimens, are transferred to Echinoplectanum, as E. plectropomi n. comb. and E. echinophallus n. comb., respectively. Six of the seven species of Echinoplectanum are parasitic in members of Plectropomus from the South West Pacific, but one (E. echinophallus) is a parasite of Epinephelus marginatus and has been recorded only from the Mediterranean and East Atlantic; it is suggested that Echinoplectanum is associated with Plectropomus, a basal genus among the epinephelines, and that host-switching to Epinephelus marginatus occurred, whose distribution extends from Europe to the Indian Ocean. Morphological characteristics of the copulatory organs suggest that a "chastity belt versus spiny penis" sperm competition pattern prevails in Echinoplectanum spp.

  6. The 'Pseudorhabdosynochus cupatus group' (Monogenea: Diplectanidae) on Epinephelus fasciatus, E. howlandi, E. rivulatus and E. merra (Perciformes: Serranidae) off New Caledonia, with descriptions of Pseudorhabdosynochus cyathus n. sp. and P. calathus n. sp.

    PubMed

    Hinsinger, Damien D; Justine, Jean-Lou

    2006-06-01

    Pseudorhabdosynochus cupatus (Young, 1969) is characterised by small lamellosquamodiscs made up of central telescopic rings and peripheral rows of rodlets, and a sclerotised vagina in the shape of a thin-walled coiled tube with three accessory chambers. Two new species of Pseudorhabdosynochus are herein described from epinepheline fish off New Caledonia, South Pacific, and are considered part of the 'P. cupatus group'. P. cyathus n. sp. from Epinephelus howlandi has lamellosquamodiscs made up of four telescopic rings and four rows of separate rodlets; there are dorsal tegumental scales. This species was found only in young hosts; older hosts harbour P. venus Hinsinger & Justine, 2006. P. calathus n. sp. from Epinephelus rivulatus has lamellosquamodiscs made up of four telescopic rings and five rows of separate rodlets; the tegument is smooth. The three species, P. cupatus, P. calathus and P. cyathus, are strictly species specific; although morphologically very similar, they can be distinguished by features of the tegumental scales and measurements of the vagina and haptoral hard parts. P. melanesiensis (Laird, 1958) from E. merra is close to the 'P. cupatus group' according to the morphology of its sclerotised vagina but is distinguished by its squamodiscs which lack telescopic rings. Rare specimens found in E. merra are tentatively attributed to P. coioidesis Bu et al., 1999. A paratype of P. coioidesis is figured for comparison. E. merra, E. fasciatus, E. rivulatus and E. howlandi each have a Pseudorhabdosynochus species of the P. cupatus lineage: these are the dominant species in each host. In addition, E. merra, E. fasciatus and E. howlandi harbour a rare species of Pseudorhabdosynochus, respectively P. cf. coioidesis, P. caledonicus Justine, 2005 and P. venus; these rare species are morphologically unrelated to each other and to the 'P. cupatus group'.

  7. Sympatric species of Deretrema Linton, 1910: D. combesae n. sp. and D. combesorum n. sp. (Digenea: Zoogonidae) from the manybar goatfish Parupeneus multifasciatus (Quoy & Gaimard, 1824) (Perciformes: Mullidae) from New Caledonia.

    PubMed

    Bray, Rodney A; Justine, Jean-Lou

    2008-03-01

    Two sympatric species of Deretrema, D. combesae n. sp. and D. combesorum n. sp. are described from the manybar goatfish Parupeneus multifasciatus from off New Caledonia, South Pacific. D. combesae n. sp. does not fit any of the described Deretrema subgenera. The combination of the characters, tegumental spines, caeca reaching past the testes, the vitellarium reaching into the hindbody, much of the uterus at the level of and anterior to the gonads, a long oesophagus, testes in the hindbody and the pre-testicular ovary are not found in any of the described subgenera. D. combesorum n. sp. fits into the subgenus Deretrema (Deretrema), but differs from the described species in the sucker-ratio, eggs size, elongate shape and contiguous testes. The sympatry of these dissimilar species of Deretrema casts doubt on the value of the subgenera in Deretrema.

  8. First description of the gravid female of Philometra rubra (Leidy, 1856) (Nematoda: Philometridae), a parasite of the abdominal cavity of temperate basses Morone Spp. (Moronidae: Perciformes) in North America.

    PubMed

    Moravec, František; de Buron, Isaure; Measures, Lena

    2013-06-01

    Gravid females of the little-known nematode species Philometra rubra ( Leidy, 1856 ) (Philometridae) are described from specimens from the abdominal cavity of the striped bass, Morone saxatilis (Walbaum), in South Carolina and Canada. The specimens were studied with the use of light and scanning electron microscopy. This species is mainly characterized by the distribution and different sizes of cephalic papillae from the external circle, which is a unique feature compared to other philometrids. Other characteristic features are the shape of the posterior end of body, size and location of caudal projections, and the presence of a well-developed anterior esophageal inflation. The morphology of the male of P. rubra and the life cycle of this nematode are still unknown.

  9. Plectorhinchus caeruleonothus, a new species of sweetlips (Perciformes:
    Haemulidae) from northern Australia and the resurrection of P. unicolor (Macleay, 1883), species previously confused with P. schotaf (Forsskål, 1775).

    PubMed

    Johnson, Jeffrey W; Wilmer, Jessica Worthington

    2015-07-14

    Two distinct haemulid fishes from Australia and the Indo-Australian Archipelago respectively have long been confused with Plectorhinchus schotaf (Forsskål, 1775). Plectorhinchus caeruleonothus sp. nov. is described from 17 specimens collected off western and far northern Australia, between the Monte Bello Islands, Western Australia and Torres Strait, Queensland. It has also been confirmed outside this range by photographs taken at Ningaloo Reef and Exmouth Gulf, Western Australia, and at Claremont Isles and Lizard Island, Queensland. The new species is unique among the genus in having a combination of dorsal-fin rays XII, 18-20, lateral-line scales 56-61, gill rakers 7-9 on the upper limb and 18-20 on the lower limb of the first arch, nostrils minute, and fresh colouration in adults including body uniformly grey, cheek, opercles and posterior margin of the opercular membrane uniformly blue-grey, and rim of orbit and upper edge of maxilla dusky yellow. In contrast to its closest congeners, the juveniles have a distinctive pattern of narrow creamish-white to pale grey stripes on a dark grey to chocolate brown background on the head and body, and oblique dark stripes progressing with growth to spots on the caudal fin. Plectorhinchus unicolor (Macleay, 1883) from Japan to northern Australia is resurrected from the synonomy of P. schotaf and redescribed on the basis of the holotype and 24 non-type specimens. Plectorhinchus unicolor is most similar to P. schotaf, but can be distinguished by fresh colouration, modal dorsal and pectoral-fin ray counts and DNA barcoding. Plectorhinchus schotaf appears to be restricted to the region from southeast Africa to the Arabian Sea, including the Red Sea and Persian Gulf. Plectorhinchus griseus (Cuvier in Cuvier & Valenciennes, 1830) from Indian and Sri Lankan Seas has previously been treated as a junior synonym of P. schotaf, but in accordance with Smith (1962), is here confirmed as a valid species, readily distinguished from the latter by a concavity in the lateral profile of the snout in adults, deep body and high soft dorsal-fin ray count. Comparison of the CO1 genetic marker utilised in DNA barcoding also resulted in significant genetic divergences between the new species, P. unicolor and their closest sampled congeners. Some behavioural observations are also presented for the species treated, including aggressive interactions between individuals of the new species, the likes of which have not previously been recorded among species of Plectorhinchus.

  10. The taxonomic status of Japanese threadfin bream Nemipterus japonicus (Bloch, 1791) (Perciformes: Nemipteridae) with a redescription of this species from the south china sea based on morphology and DNA barcodes

    NASA Astrophysics Data System (ADS)

    Ning, Ping; Sha, Zhongli; Hebert, Paul D. N.; Russell, Barry

    2015-02-01

    Because of its importance as a food source, Nemipterus japonicus (Bloch, 1791) (Nemipteridae) or Japanese threadfin bream is the best studied of these taxa, and numerous investigations have examined its fisheries, its biology and biochemistry. Despite such intensive work, the taxonomic status of N. japonicus has never been seriously questioned and it is regarded as a common species, widely distributed throughout the Indo-Western Pacific Ocean. In fact, Bloch's description of the type specimen of N. japonicus has ambiguous collection data and lacks a designation for the type locality, though it is probably Java. In this paper, DNA barcode results based on COI gene support the existence of two geographically separated lineages of the Japanese threadfin bream, both being an Indian Ocean and western Pacific lineage, with 2.7% sequence divergence, and the results indicate a possible existing of some cryptic species. The two lineages also possess a diagnostic difference in their belly color, with specimens in the South China Sea having a silver belly, while those from the Indian Ocean isolate specimen have a yellow coloration. Based upon new collections from the South China Sea, this species from the western Pacific is morphologically redescribed and its details of DNA barcode diversity are shown for the future investigations.

  11. Rediscovery of Sagittalarva inornata n. gen., n. comb. (Gilbert, 1890) (Perciformes: Labridae), a long-lost deepwater fish from the eastern Pacific Ocean: a case study of a forensic approach to taxonomy using DNA barcoding.

    PubMed

    Victor, Benjamin C; Alfaro, Michael E; Sorenson, Laurie

    2013-01-01

    Some of the more valuable contributions of a standardized DNA sequence database (the DNA barcode) are matching specimens of different life stages and confirming the species identity of individuals from distant locations. These applications can facilitate the detective work required to solve difficult taxonomic problems. In this case, a match was made between the COI mtDNA sequence of an adult male wrasse recently caught at the tip of Baja California in Mexico in deep water (30-100m) and sequences from a series of unusual larvae collected about 3500 km to the south, in the open ocean over the Galápagos Rift hydrothermal vents in 1985. The Baja adults fit the recent description of Halichoeres raisneri Baldwin & McCosker, 2001 from the Galápagos and Cocos Islands. However, another deepwater labrid is known from the same site and depth in Baja; it is the type locality for the century-old holotype and only specimen of the Cape Wrasse Pseudojulis inornatus Gilbert, 1890 (later as Pseudojuloides inornatus). Deepwater video images from the tip of Baja show wrasses identical to H. raisneri photographed in Galápagos but who also fit the description of Pseudojulis inornatus. This coincidence led to a closer investigation of the holotype with x-ray, which revealed unanticipated caniniform teeth (vs. incisiform in Pseudojuloides) and an error in the fin-ray count in the original description, both of which mistakenly separated Halichoeres raisneri. The two species now match in markings, meristics, and morphology as well as overlapping range and are therefore synonymized. Phenetic and phylogenetic trees using mtDNA and nuclear DNA sequences show the species is not close to any other lineage and does not group with the other julidine labrids of the New World or the Pseudojuloides or Halichoeres of the Indo-Pacific. The distinctive larval morphology, long, thin, and flattened with a sharply pointed black-tipped snout, resembles no other described labrid larvae and, without an available genus, the new genus Sagittalarva Victor, n. gen. and the new combination Sagittalarva inornata (Gilbert, 1890), n. gen., n. comb. are described.

  12. Molecular identification of cryptic species of Ceratomyxa Thélohan, 1892 (Myxosporea: Bivalvulida) including the description of eight novel species from apogonid fishes (Perciformes: Apogonidae) from Australian waters.

    PubMed

    Heiniger, Holly; Adlard, Robert D

    2013-09-01

    Ceratomyxa parasites from the gall bladders of 23 species of cardinalfishes (family Apogonidae) from Australian waters were examined for their taxonomic identity and phylogenetic relatedness. We identified 15 of the 23 apogonid fish species infected with species of Ceratomyxa. Although the majority of apogonid species harboured only a single Ceratomyxa species, four were found with multiple species of Ceratomyxa. This study describes eight novel species using a combination of morphological, small subunit ribosomal DNA (SSU rDNA) and biological characters. Six Ceratomyxa species are reported from single apogonid species, while two are reported from multiple host species. Molecular data were critical in identifying several morphologically cryptic species. However, our results suggest that SSU rDNA was not capable of distinguishing all the species present in the current study system and alternative genetic markers should be investigated in the future.

  13. Comet assay and micronucleus tests on Oreochromis niloticus (Perciforme: Cichlidae) exposed to raw sugarcane vinasse and to phisicochemical treated vinasse by pH adjustment with lime (CaO).

    PubMed

    Correia, Jorge E; Christofoletti, Cintya Ap; Ansoar-Rodríguez, Yadira; Guedes, Thays A; Fontanetti, Carmem S

    2017-04-01

    In Brazil vinasse, a main sugarcane distillery residue, stands out because every liter of alcohol generates 10-15 L of vinasse as waste. An alternative for the disposal of this waste is the fertirrigation of the sugarcane culture itself. However, the high amount released can saturate the soil and through leaching/percolation contaminate water resources. The aim of this study is verifying the toxic potential of vinasse in tilapias and effectiveness of the physicalchemical treatment of this waste with pH adjustment with lime (CaO). The comet assay and the micronucleus test were applied on animals exposed to dilutions of raw vinasse and vinasse adjusted to neutral pH. Bioassays with raw vinasse dilutions indicated a toxic and genotoxic potential; fish exposed to the highest concentration died less than 48 h after the exposure; the incidence of micronucleus was significantly higher when compared to negative control for all dilutions. For the comet assay, the scores of damage were statistically higher for all dilutions, with the exception of the 1% dillution. However, in the bioassay with the chemically treated vinasse (neutral pH), most fish in the 10% dilution survived and there was no significant difference when compared to the control. Damage scores in the comet assay were similar to the results of the untreated vinasse. The chemical treatment of vinasse with lime to neutralize the pH proved to be an effective alternative for the toxicity reduction of this residue, since it reduced the mortality of fish at higher concentrations and the incidence of damage to DNA. Copyright © 2017 Elsevier Ltd. All rights reserved.

  14. Morphology and histopathology of Calyptospora sp. (Apicomplexa: Calyptosporidae) in speckled peacock bass, Cichla temensis Humboldt, 1821 (Perciformes: Cichlidae), from the Marajó-Açu River, Marajó Island, Brazil.

    PubMed

    Santiago, Hérika; Corrêa, José Luís; Tortelly, Rogerio; Menezes, Rodrigo Caldas; Matos, Patrícia; Matos, Edilson

    2012-06-01

    Several species of coccidia are protozoan parasites that cause infection in a wide variety of animal groups. Calyptospora is an important genus of protozoan, which infests both freshwater and marine fish. The hepatopancreases of 150 speckled peacock bass captured on Marajó Island, Brazil were studied macro- and microscopically. Oocysts were found in 84 (56%) of the specimens in both the examination of the fresh material by compression and the analysis of histological sections stained with hematoxylin-eosin. Small, circular, homogeneous forms in negative contrast had a mean diameter of 21.2 μm, frequently with pyriform sporocysts, with a mean length of 9.2 μm and width of 3.1 μm, and a thin-walled capsule, were observed in both the hepatic and the pancreatic parenchyma, but were completely devoid of any inflammatory reaction. Calyptospora infections are documented for the first time in the Marajó-Açu River.

  15. Ciguatera incidence and fish toxicity in Okinawa, Japan.

    PubMed

    Oshiro, Naomasa; Yogi, Kentaro; Asato, Shuko; Sasaki, Toshiki; Tamanaha, Koji; Hirama, Masahiro; Yasumoto, Takeshi; Inafuku, Yasuo

    2010-10-01

    Okinawa being located in the subtropical region has the highest incidence of ciguatera in Japan. Officially, 33 outbreaks involving 103 patients have been reported between 1997 and 2006. The implicated species were Variola louti, Lutjanus bohar, Lutjanus monostigma, Epinephelus fuscoguttatus, unidentified Lutjanus sp., Plectropomus areolatus, Oplegnathus punctatus, Epinephelus polyphekadion, Caranx ignobilis and moray eel. Toxicities of the leftover meals, as determined by mouse bioassays, ranged from 0.025 to 0.8 MU/g or above (equivalent to 0.175-5.6 ngCTX1B/g). We collected 612 specimens of fish belonging to L. monostigma, L. bohar, Lutjanus argentimaculatus, Lutjanus russellii, V. louti, Variola albimarginata, and E. fuscoguttatus from the coasts around Okinawa and examined the toxicity of the flesh by the mouse bioassay. The rate of toxic fish was as follows: L. monostigma: 32.3%, L. bohar: 11.9%, V. louti: 14.3%, E. fuscoguttatus: 20.8%. Only one out of 36 samples of V. albimarginata and two of 74 samples of L. russellii were found toxic. None of the 35 samples of L. argentimaculatus was toxic. Nor the L. bohar samples weighing less than 4 kg were toxic. In all toxic samples, CTX1B was detected by LC/MS analysis but CTX3C and 51-hydroxyCTX3C were not. Copyright 2009 Elsevier Ltd. All rights reserved.

  16. A monogenean fish parasite, Gyrodactylus chileani n. sp., belonging to a novel marine species lineage found in the South-Eastern Pacific and the Mediterranean and North Seas.

    PubMed

    Ziętara, Marek S; Lebedeva, Dar'ya; Muñoz, Gabriela; Lumme, Jaakko

    2012-10-01

    Gyrodactylus chileani n. sp. is the first Gyrodactylus species reported from Chile. It is an ectoparasite living on fins and skin of a small fish, the Chilean tidal pond dweller Helcogrammoides chilensis (Cancino) (Perciformes: Tripterygiidae). A phylogenetic analysis based on 5.8S+ITS2 of rDNA placed the new species close to marine Gyrodactylus species found in Europe: G. orecchiae Paladini, Cable, Fioravanti, Faria, Cave & Shinn, 2009 on gilthead seabream Sparus aurata L. from the Adriatic and Tyrrhenian Sea fish farms (Perciformes: Sparidae), and an undescribed species on the black goby Gobius niger L. from the North Sea (Perciformes: Gobiidae). A morphological description of the latter species is unavailable. These geographically distant parasite samples on different host families form a new well supported Gyrodactylus orecchiae lineage. Using molecular phylogenetics, it is shown that the marine species groups of Gyrodactylus may have a worldwide distribution.

  17. Feeding ecology of some fish species occurring in artisanal fishery of Socotra Island (Yemen).

    PubMed

    Hassan Ali', Mohammed Kaed; Belluscio, Andrea; Ventura, Daniele; Ardizzone, Giandomenico

    2016-04-30

    The demersal species Lethrinus borbonicus, Lethrinus mahsena, Lethrinus microdon, Lethrinus nebulosus, Lutjanus bohar, Lutjanus gibbus, Lutjanus kasmira, Epinephelus fasciatus, Epinephelus stoliczkae, Carangoides gymnostethus and Euthynnus affinis are important coastal fishes species of the northern coast of Socotra (Yemen), exploited by local fishery. The biology and feeding ecology of these species are poorly known in the area. A total of 1239 specimens were sampled from the main fishing landing site of the island (Hadibo). Total length and weight were measured, stomach contents were analyzed, diet overlap, Fulton's Condition index, and trophic levels were estimated. C. gymnostethus, L. microdon and L. kasmira occupied the highest position (T=4.50), L. nebulosus occupied the lower one (TL=3.41). The role of the increasing abundance of small pelagic fish in the diet of many species after the upwelling event is evident, but also different feeding strategies are reported, according to fish ecology. Copyright © 2016 Elsevier Ltd. All rights reserved.

  18. [Anisakis simplex larvae: infection status in marine fishes for sale in Shantou].

    PubMed

    Chen, Jun-Hua; Xu, Zhi-Xia; Xu, Guang-Xing; Huang, Jian-Yun; Chen, Hong-Hui; Shi, Shi-Zun; Wu, Xiu-Yang; Liang, Jing-Jing

    2014-06-01

    To investigate the infection status of Anisakis simplex larvae in marine fishes for sale in Shantou. Marine fishes were randomly collected from markets in Shantou City from February to December 2013, and then classified. The viscera and muscle of each fish were carefully dissected and thoroughly examined for anisakids. The larvae were examined under a light microscope. The infection rate and intensity of Anisakis simplex larvae were calculated. A total of 382 fish specimens belonging to 52 species were examined. 42 out of 52 species (80.8%) were found infected by A. simplex larvae. The overall infection rate reached 47.4% (181/382), and average 5.5 larvae parasitized per infected fish (995/181). The survival rate of larvae was 100%. The highest infection rate observed was 100% in Scomber australasicus (4/4), Trachurus japonicus (9/9), Decapterus maruadsi (8/8), Lutjanus lutjanus (9/9), Argyrosomus argentatus (4/4), Nibea albiflora (4/4), Nemipterus bathybius (12/12), Trachinocephalus myops (7/7) and Mene maculata (9/9), followed by 16/18 in Pneumatophorus japonicus, 6/7 in Lutjanus ophuysenii and 5/6 in Lutjanus fulvus. A. simplex larvae were not detected in 10 fish species, namely, Megalaspis cordyla, Lutjanus argentimaculatus, Lutjanus fulviflamma, Acanthopagrus australis, Acanthopagrus latus, Plectorhinchus nigrus, Dentex tumifrons, Psenopsis anomala, Scatophagus argus, and Seriola lalandi. The infection intensity was the highest in Lutjanus fulvus (21.0 per fish), followed by Trachinocephalus myops (16.7 per fish), Saurida filamentosa (14.0 per fish) and Mene maculate (10.1 per fish). The lowest infection intensity was found in Rastrelliger kanagurta, Kaiwarinus equula, Atule mate, Lutjanus russellii, Plectorhinchus cinctus, Priacanthus tayenus, Branchiostegus argentatus, Branchiostegus albus, Sphyraena pinguis, Formio niger, Trachinotus blochii, Siganus fuscescens and Choerodon azurio (less than 2 per fish). The highest infection rate (34.3%, 131/382) was

  19. Centrarchid identification and natural history

    Treesearch

    M.L. Warren

    2009-01-01

    The family Centrarchidae (Order: Perciformes) is one of the most diverse, widespread, and conspicuous fish families native to freshwater habitats of North America. Among endemic fish families of North America, only the North American catfish family (Ictaluridae) has more species. The family name, Centrarchidae, refers to the anal fin spines of species in the family,...

  20. CHEMICAL INTERFERENCE WITH GENOMIC AND NONGENOMIC ACTIONS OF STEROIDS IN FISHES: ROLE OF RECEPTOR BINDING. (R826125)

    EPA Science Inventory

    Abstract

    The characteristics of steroid nuclear and membrane receptors and their interactions with xenobiotic chemicals in two marine perciform species, Atlantic croaker (Micropogonias undulatus) and spotted seatrout (Cynoscion nebulosus) are briefly revi...

  1. Does lymphocystis occur in pacora, Plagioscion surinamensis (Sciaenidae), from Colombia?

    PubMed

    Bunkley-Williams, Lucy; Williams, Ernest H; Phelps, Ronald P

    2002-04-01

    A lymphocystis-like condition produced hemorrhagic lesions over the bodies of pacora Plagioscion surinamensis (Bleeker) (Perciformes: Sciaenidae) held in freshwater aquaculture in northern Colombia and increased their sensitivity to handling stress. This disease eliminated this fish from a project evaluating its aquaculture potential. Lymphocystis disease is assumed to be less damaging to the host. This condition requires more study and histological evaluation.

  2. 77 FR 27716 - Fishery of the Gulf of Mexico; Southeast Data, Assessment, and Review (SEDAR); Public Meetings

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-11

    ... (Lutjanus Campechanus) data scoping assessment process webinar. SUMMARY: The SEDAR 31 assessment of the Gulf... notice is for a webinar associated with the Data portion of the SEDAR process. See SUPPLEMENTARY INFORMATION. DATES: The SEDAR 31 Data Workshop data scoping webinar will be held on July 11, 2012, 1 p.m. to 5...

  3. A Study of Some Problems in Network Information Theory

    ERIC Educational Resources Information Center

    Kamath, Sudeep Uday

    2013-01-01

    Red snapper, "Lutjanus campechanus," were sampled with hook and line at natural (n = 33) and artificial (n = 27) reef sites in the northern Gulf of Mexico from 2009-2011. Stomachs (n = 708) were extracted and their contents preserved for gut content analysis, and muscle tissue samples (n = 200) were dissected and frozen for stable…

  4. Fisheries Management of Red Snapper in the Gulf of Mexico: A Case Study

    ERIC Educational Resources Information Center

    Smith, Joy N.; Snyder, Stephanie M.; Berkson, Jim; Murphy, Brian R.; McMullin, Steve L.

    2009-01-01

    In the Gulf of Mexico, the overfished population of red snapper ("Lutjanus campechanus") is a major source of revenue resulting in a dichotomy between maintaining the health of the fishery and meeting the demands of the local economies. In order to govern marine fisheries the Magnuson-Stevens Fishery Conservation and Management Act of…

  5. Fisheries Management of Red Snapper in the Gulf of Mexico: A Case Study

    ERIC Educational Resources Information Center

    Smith, Joy N.; Snyder, Stephanie M.; Berkson, Jim; Murphy, Brian R.; McMullin, Steve L.

    2009-01-01

    In the Gulf of Mexico, the overfished population of red snapper ("Lutjanus campechanus") is a major source of revenue resulting in a dichotomy between maintaining the health of the fishery and meeting the demands of the local economies. In order to govern marine fisheries the Magnuson-Stevens Fishery Conservation and Management Act of…

  6. A Study of Some Problems in Network Information Theory

    ERIC Educational Resources Information Center

    Kamath, Sudeep Uday

    2013-01-01

    Red snapper, "Lutjanus campechanus," were sampled with hook and line at natural (n = 33) and artificial (n = 27) reef sites in the northern Gulf of Mexico from 2009-2011. Stomachs (n = 708) were extracted and their contents preserved for gut content analysis, and muscle tissue samples (n = 200) were dissected and frozen for stable…

  7. Species of Pseudorhabdosynochus Yamaguti, 1958 (Monogenea: Diplectanidae) from Epinephelus fasciatus and E. merra (Perciformes: Serranidae) off New Caledonia and other parts of the Indo-Pacific Ocean, with a comparison of measurements of specimens prepared using different methods, and a description of P. caledonicus n. sp.

    PubMed

    Justine, Jean-Lou

    2005-09-01

    Species of Pseudorhabdosynochus were studied from fresh specimens collected from Epinephelus fasciatus and E. merra off New Caledonia, South Pacific, and specimens deposited in Museums. Experiments on two species demonstrated that the sclerotised hollow organs, such as the quadriloculate male copulatory organ and the vagina, may show differences in measurements of up to 50% when flattened. P. caledonicus n. sp. is described from E. fasciatus in New Caledonia, on which it is relatively rare; it is distinguished on the basis of the quadriloculate organ, which has a very thin anterior wall, the sclerotised parts of the vagina in form of a straight tube with a star-shaped lateral structure, and the squamodiscs composed of 11 open rows of rodlets. P. cupatus (Young, 1969) is redescribed from abundant material from E. fasciatus off New Caledonia (new geographical record) and compared with paratype specimens from Australia (from E. fasciatus and E. merra) and specimens from E. fasciatus in the Red Sea (both herein redescribed and figured); a specimen was also found on a slide from E. merra off Vanuatu. P. melanesiensis (Laird, 1958) is redescribed from material from E. merra off New Caledonia (new geographical record) and compared with type-specimens (herein redescribed and figured) from the same host off Vanuatu. The structure of the sclerotised vagina in P. cupatus and P. melanesiensis is very similar, with a thin-walled tube and a heavily sclerotised structure with three loculi. P. vagampullum (Young, 1969) is redescribed from the paratypes from E. merra from Australia, but was not found in New Caledonia; specimens included among its paratypes (from E. merra in Australia), but different, are herein attributed to Pseudorhabdosynochus sp. 3. P. lantauensis (Beverley-Burton & Suriano, 1981) is redescribed from the paratype specimens from E. longispinis off Hong-Kong. A specimen found among the paratypes of P. cupatus belongs to a different species, herein designated as Pseudorhabdosynochus sp. 1. Specimens from E. longispinis off Hong-Kong, previously attributed to P. cupatus, are attributed to another species, Pseudorhabdosynochus sp. 2. The three species P. cupatus, Pseudorhabdosynochus sp. 1 and Pseudorhabdosynochus sp. 2 have in common a 'lamellosquamodisc' composed of central telescopic lamellae and peripheral rows of rodlets; they can be distinguished by the shape of the sclerotised vagina and measurements of the haptoral hard-parts. Specimens from E. longispinis off Hong-Kong, previously attributed to P. vagampullum, probably belong to a different species. Consequently, after these modified determinations, P. cupatus parasitises only E. fasciatus and E. merra, and P. melanesiensis and P. vagampullum parasitise only E. merra. With their wide geographical distribution and different species of Pseudorhabdosynochus in different localities, E. fasciatus and E. merra appear to represent excellent models for investigating monogenean biogeography in the Indo-Pacific Ocean.

  8. Kudoa spp. (Myxozoa, Multivalvulida) parasitizing fish caught in Aracaju, Sergipe, Brazil.

    PubMed

    Eiras, Jorge Costa; Fujimoto, Rodrigo Yudi; Madi, Rubens Riscala; Jeraldo, Veronica de Lourdes Sierpe; Melo, Cláudia Moura de; Souza, Jônatas Dos Santos de; Diniz, José Antonio Picanço; Diniz, Daniel Guerreiro

    2016-01-01

    This study reports on Kudoa spp. (Myxozoa, Multivalvulida) from the fish species Lutjanus analis, Bagre marinus, Aspistor luniscutis and Lutjanus jocu, which were caught in Aracaju, state of Sergipe, Brazil. The parasites formed oval plasmodia around the esophagus of L. analis, and elongated plasmodia inside the skeletal muscle of B. marinus, A. luniscutis and L. jocu. Host myoliquefaction was not observed in all the cases studied. The current study provides a morphological and morphometric description of each parasite as well as a comparison with all the species described worldwide. Lack of molecular data impaired specific identification of the parasites. The importance of these parasites is discussed and the need for further studies on infections in Brazilian fish is emphasized because of the high economic impact of some Kudoa species which cause liquefaction in hosts' muscles and render these fish unsuitable for consumption.

  9. Environmental Assessment of Installation Development at MacDill Air Force Base, Florida

    DTIC Science & Technology

    2007-01-01

    Habitat Fish Common snook a Centropomus undecimalis NL SSC Uses mangroves for spawning. Mammals Florida mouse Podomys floridanus NL SSC Prefers...gopher frog, Florida mouse , and burrowing owl) because other species use inactive gopher tortoise burrows for shelter (GTC 2001). Removal and...Lutjanus synagris -- X X -- Red drum Sciaenops ocellatus -- X X X Spanish mackerel Scomberomorus maculatus -- X -- X Spiny lobster Panulirus argus

  10. Location of volatile odor sources by ghost crabOcypode quadrata (Fabricius).

    PubMed

    Wellins, C A; Rittschof, D; Wachowiak, M

    1989-04-01

    The ghost crab,Ocypode quadrata, was tested in the field for its ability to locate sources of volatile cues. The pure compound skatole, 3-methylindole, was a potent attractant. Crabs also located sources of complex odors such as dead fish,Lutjanus campechanus, dead mole crabs,Emerita talpoida; and peeled bananas. Ghost crabs possess concealed and reduced antennules that may not be the primary olfactory organs. Chemosensory hairs borne on the dactyls may be the primary detection system.

  11. Observations on cucullanid nematodes from freshwater fishes in Mexico, including Dichelyne mexicanus sp. n.

    PubMed

    Caspeta-Mandujano, J M; Moravec, F; Salgado-Maldonado, G

    1999-01-01

    A new cucullanid nematode, Dichelyne mexicanus sp. n., is described from the intestine of three species of fishes, Agonostomus monticola (Bancroft) (Mugilidae, Perciformes) (type host), Ictalurus balsanus (Jordan et Snyder) (Ictaluridae, Siluriformes) and Cichlasoma beani (Jordan) (Cichlidae, Perciformes), from three rivers (La Maquina River, Veracruz; Chontalcoatlán River, Guerrero and Santiago River, Nayarit) in central Mexico. This species is characterised by the absence of a ventral sucker in the male (subgenus Dichelyne) and it differs from its congeners mainly in possessing very unequal and dissimilar spicules (left 0.465-0.768 mm and right 293-548 mm long), an asymmetrical gubernaculum, and two intestinal caeca. Another cucullanid nematode, Cucullanus caballeroi Petter, 1977, is reported from Dormitator maculatus (Bloch) (Eleotridae, Perciformes) from the La Palma and La Maquina Rivers and Balzapote stream, Veracruz, being briefly described and illustrated; this represents a new host record. Findings of D. mexicanus and C. caballeroi represent a new record of cucullanid nematodes from fishes in Mexican fresh waters.

  12. Systematic revision of the pennellid genus Creopelates Shiino, 1958 (Copepoda: Siphonostomatoida) and the proposal of a new genus.

    PubMed

    Uyeno, Daisuke

    2015-01-07

    The parasitic copepod Creopelates floridus Shiino, 1958 (Siphonostomatoida: Pennellidae) is redescribed based on postmetamorphic adult females in the collection of the Imperial Majesty of Japan deposited in the National Museum of Nature and Science, Tsukuba (NSMT), Japan. Five new species of pennellid copepods are described based on postmetamorphic adult females from marine actinopterygian fishes newly collected in littoral waters of Japan and the Philippines, in the western North Pacific. The copepods and their hosts are as follows: Creopelates hosinoi n. sp. from Bryaninops yongei (Davis & Cohen) (Perciformes: Gobiidae); C. shirakawai n. sp. from Diancistrus fuscus (Fowler) (Ophidiiformes: Bythitidae); C. lubangenesis n. sp. from Gobiodon rivulatus (Rüppell) (Perciformes: Gobiidae); Nagasawanus akinohama n. gen. et n. sp. from Trimma grammistes (Tomiyama) (Perciformes: Gobiidae); N. snufkini n. gen. et n. sp. from T. tevegae Cohen & Davis. The total number of valid species contained in the genus Creopelates is now five. Nagasawanus n. gen. is distinguishable from other pennellid genera by the following features: antennary processes and cephalic lobes rounded without branched fringes, neck region without processes, maxilla with claw-like terminal segment lacking spinules. Keys to the genera of Pennellidae and to the species of Creopelates and Nagasawanus n. gen. are also provided.

  13. Dependence of juvenile reef fishes on semi-arid hypersaline estuary microhabitats as nurseries.

    PubMed

    Sales, N S; Dias, T L P; Baeta, A; Pessanha, A L M

    2016-07-01

    The differences between fish assemblages in three microhabitat types, in relation to vegetation and sediment characteristics of a hypersaline estuary located in an semi-arid zone in north-eastern Brazil, were investigated. Fishes were collected using a beach seine during the rainy and dry seasons in 2012. A total of 78 species were recorded, with the most common families being Gerreidae, Lutjanidae and Tetraodontidae. The majority of species were represented by juveniles, with Eucinostomus argenteus, Ulaema lefroyi and Sphoeroides greeleyi being the dominant species. The fish assemblage structures differed significantly among microhabitat types, with the narrow intertidal flat adjacent to the mangrove fringe supporting the most diverse fish fauna. In addition, only 27 species were common to all of the microhabitats. The results support the hypothesis that hypersaline estuaries serve as important nursery areas for various reef fish species, due to the structural complexity provided by their macroalgae beds and mangroves. © 2016 The Fisheries Society of the British Isles.

  14. Cucullanus maldivensis n. sp. (Nematoda: Cucullanidae) and some other adult nematodes from marine fishes off the Maldive Islands.

    PubMed

    Moravec, Frantisek; Lorber, Julia; Konecný, Robert

    2008-05-01

    Marine fishes were collected from off the Maldive Islands in March, 2005. From amongst the material collected, the nematode Cucullanus maldivensis n. sp. is described from the intestine of a lutjanid fish, the black and white snapper Macolor niger (Forsskål). This species is morphologically and biometrically most similar to C. bourdini Petter & Le Bel, 1992, differing from it principally in the protruding vulval lips, the location of the first pair of pre-anal papillae, the absence of an elevated cloacal region, and having distinctly larger eggs (51-57 x 33-36 microm). Additionally, adult females of the nematodes Procamallanus (Spirocamallanus) sp. and Camallanus sp. from the green jobfish Aprion virescens Valenciennes (Lutjanidae) and the rainbow runner Elegatis bipinnulata (Quoy & Gaimard) (Carangidae), respectively, were collected. These camallanids are illustrated and measurements are provided, but they were not identified or described in detail as no males were collected.

  15. Phylogenetic Diversity and Cosymbiosis in the Bioluminescent Symbioses of “Photobacterium mandapamensis”▿ †

    PubMed Central

    Kaeding, Allison J.; Ast, Jennifer C.; Pearce, Meghan M.; Urbanczyk, Henryk; Kimura, Seishi; Endo, Hiromitsu; Nakamura, Masaru; Dunlap, Paul V.

    2007-01-01

    “Photobacterium mandapamensis” (proposed name) and Photobacterium leiognathi are closely related, phenotypically similar marine bacteria that form bioluminescent symbioses with marine animals. Despite their similarity, however, these bacteria can be distinguished phylogenetically by sequence divergence of their luminescence genes, luxCDAB(F)E, by the presence (P. mandapamensis) or the absence (P. leiognathi) of luxF and, as shown here, by the sequence divergence of genes involved in the synthesis of riboflavin, ribBHA. To gain insight into the possibility that P. mandapamensis and P. leiognathi are ecologically distinct, we used these phylogenetic criteria to determine the incidence of P. mandapamensis as a bioluminescent symbiont of marine animals. Five fish species, Acropoma japonicum (Perciformes, Acropomatidae), Photopectoralis panayensis and Photopectoralis bindus (Perciformes, Leiognathidae), Siphamia versicolor (Perciformes, Apogonidae), and Gadella jordani (Gadiformes, Moridae), were found to harbor P. mandapamensis in their light organs. Specimens of A. japonicus, P. panayensis, and P. bindus harbored P. mandapamensis and P. leiognathi together as cosymbionts of the same light organ. Regardless of cosymbiosis, P. mandapamensis was the predominant symbiont of A. japonicum, and it was the apparently exclusive symbiont of S. versicolor and G. jordani. In contrast, P. leiognathi was found to be the predominant symbiont of P. panayensis and P. bindus, and it appears to be the exclusive symbiont of other leiognathid fishes and a loliginid squid. A phylogenetic test for cospeciation revealed no evidence of codivergence between P. mandapamensis and its host fishes, indicating that coevolution apparently is not the basis for this bacterium's host preferences. These results, which are the first report of bacterial cosymbiosis in fish light organs and the first demonstration that P. leiognathi is not the exclusive light organ symbiont of leiognathid fishes

  16. Phylogenetic diversity and cosymbiosis in the bioluminescent symbioses of "Photobacterium mandapamensis".

    PubMed

    Kaeding, Allison J; Ast, Jennifer C; Pearce, Meghan M; Urbanczyk, Henryk; Kimura, Seishi; Endo, Hiromitsu; Nakamura, Masaru; Dunlap, Paul V

    2007-05-01

    "Photobacterium mandapamensis" (proposed name) and Photobacterium leiognathi are closely related, phenotypically similar marine bacteria that form bioluminescent symbioses with marine animals. Despite their similarity, however, these bacteria can be distinguished phylogenetically by sequence divergence of their luminescence genes, luxCDAB(F)E, by the presence (P. mandapamensis) or the absence (P. leiognathi) of luxF and, as shown here, by the sequence divergence of genes involved in the synthesis of riboflavin, ribBHA. To gain insight into the possibility that P. mandapamensis and P. leiognathi are ecologically distinct, we used these phylogenetic criteria to determine the incidence of P. mandapamensis as a bioluminescent symbiont of marine animals. Five fish species, Acropoma japonicum (Perciformes, Acropomatidae), Photopectoralis panayensis and Photopectoralis bindus (Perciformes, Leiognathidae), Siphamia versicolor (Perciformes, Apogonidae), and Gadella jordani (Gadiformes, Moridae), were found to harbor P. mandapamensis in their light organs. Specimens of A. japonicus, P. panayensis, and P. bindus harbored P. mandapamensis and P. leiognathi together as cosymbionts of the same light organ. Regardless of cosymbiosis, P. mandapamensis was the predominant symbiont of A. japonicum, and it was the apparently exclusive symbiont of S. versicolor and G. jordani. In contrast, P. leiognathi was found to be the predominant symbiont of P. panayensis and P. bindus, and it appears to be the exclusive symbiont of other leiognathid fishes and a loliginid squid. A phylogenetic test for cospeciation revealed no evidence of codivergence between P. mandapamensis and its host fishes, indicating that coevolution apparently is not the basis for this bacterium's host preferences. These results, which are the first report of bacterial cosymbiosis in fish light organs and the first demonstration that P. leiognathi is not the exclusive light organ symbiont of leiognathid fishes

  17. Prediction and verification of possible reef-fish spawning aggregation sites in Los Roques Archipelago National Park, Venezuela.

    PubMed

    Boomhower, J; Romero, M; Posada, J; Kobara, S; Heyman, W

    2010-09-01

    This study attempts to predict and verify possible spawning aggregation sites and times in the Los Roques Archipelago National Park, Venezuela, based on physical reef characteristics and the knowledge of experienced local fishermen. Three possible aggregation sites were selected for monitoring based on satellite images, low-cost bathymetric mapping and interviews with experienced local fishermen. Abundances and sizes of 18 species that are known to form reproductive aggregations were monitored at these sites using underwater visual census for 7 days after each full moon from February to August, 2007. While spawning events were not observed, possible indirect evidence of spawning aggregations was found for Lutjanus analis at Cayo Sal and Boca de Sebastopol, Lutjanus apodus at Cayo Sal, Lutjanus cyanopterus at Cayo Sal and Piedra La Guasa and Epinephelus guttatus at Bajo California and Cayo de Agua. Additionally, indirect evidence was identified for the past existence of a spawning aggregation of Epinephelus striatus in the northern part of the archipelago, which may have been eliminated by overfishing c.15 years ago. Bathymetric mapping showed that the shelf edge at sites monitored in this study was shallower than at spawning aggregation sites in other parts of the Caribbean, and that sites were not proximal to deep water. While this study does not prove the existence or locations of spawning aggregations of reef fishes in the archipelago, it does add insight to a growing understanding of generalities in the relationship between seafloor characteristics and the locations of transient reef-fish spawning aggregations in the Caribbean. © 2010 The Authors. Journal compilation © 2010 The Fisheries Society of the British Isles.

  18. Cloning and sequencing of dolphinfish (Coryphaena hippurus, Coryphaenidae) growth hormone-encoding cDNA.

    PubMed

    Peduel, A D; Elizur, A; Knibb, W

    1994-01-01

    The cDNA encoding the preprotein growth hormone from the dolphinfish (Coryphaena hippurus) has been cloned and sequenced. The cDNA was derived by reverse transcription of RNA from the pituitary of a young fish using the method known as Rapid Amplification of cDNA Ends (RACE). An oligonucleotide primer corresponding to the 5' region of Pagrus major and the universal RACE primer enabled amplification using the Polymerase Chain Reaction (PCR). The dolphinfish and yellow-tail, Seriola quineqeradiata, are both members of the sub-order Percoidei (Perciforme) and their GH sequences show a high level of homology.

  19. Chemical biology of the mutualistic relationships of sea anemones with fish and crustaceans.

    PubMed

    Mebs, Dietrich

    2009-12-15

    Fish species of the genera Amphiprion and Premnas (Perciformes: Pomacentridae) as well as various crustaceans seek protection from predators among the tentacles of sea anemones, where they live essentially unharmed from stinging by the host's nematocysts. The mucous coats of anemonefish and crustaceans have been suggested as mechanisms that prevent the discharge of the nematocysts upon contact. Whereas some fish species seem to produce their own protecting mucous coating, others may acquire mucus from the sea anemone during an acclimation period, as crustaceans do. Whether the natural or acquired mucus layers contain components that inhibit nematocyst discharge or simply lack compounds stimulating the stinging cell's exocytosis is still unknown.

  20. Meagre Argyrosomus regius (Osteichthyes) as host of a gonad-infecting species of Philometra (Nematoda: Philometridae) off the Atlantic coast of Portugal.

    PubMed

    Moravec, Frantisek; Prista, Nuno; Costa, Maria José

    2007-10-31

    Subgravid females (up to 439 mm long) of the nematode Philometra sp. were found in meagre Argyrosomus regius (Asso, 1801) (Sciaenidae: Perciformes) off the southern Atlantic coast of Portugal in 2006. The general morphology of these nematodes somewhat resembles that of Philometra lateolabracis (Yamaguti, 1935), but the gravid females of the species from A. regius are apparently much longer. This is the first documented record of a gonad-infecting species of Philometra in marine fishes off the Atlantic coast of Europe. The possible importance of the gonad-parasitizing Philometra spp. as pathogens of marine fishes is stressed.

  1. Shipboard assessment of hearing sensitivity of tropical fishes immediately after exposure to seismic air gun emissions at Scott Reef.

    PubMed

    Hastings, Mardi C; Miksis-Olds, Jennifer

    2012-01-01

    This is the first study to examine the potential effects of emissions from an air gun array on hearing of tropical reef fish using AEP thresholds measured in the field. Over 7 days, 51 Chromis viridis,47 Lutjanus kasmira, 20 Myripristis murdjan, and 10 Sargocentron spiniferum were tested. AEP thresholds were determined before and after exposure to emissions from one or two passes of an airgun array. Temporary threshold shift resulting from sound exposure was not found in any species,even when cumulative sound exposure levels reached 190 dB re 1 μPa2-s.

  2. Fish assemblages associated with oil industry structures on the continental shelf of north-western Australia.

    PubMed

    Pradella, N; Fowler, A M; Booth, D J; Macreadie, P I

    2014-01-01

    This study provides the first assessment of fish associations with oil and gas structures located in deep water (85-175 m) on Australia's north-west continental shelf, using rare oil industry video footage obtained from remotely operated vehicles. A diverse range of taxa were observed associating with the structures, including reef-dependent species and transient pelagic species. Ten commercially fished species were observed, the most abundant of which was Lutjanus argentimaculatus, with an estimated biomass for the two deepest structures (Goodwyn and Echo) of 109 kg.

  3. Role of histamine in the regulation of intestinal immunity in fish.

    PubMed

    Galindo-Villegas, Jorge; Garcia-Garcia, Erick; Mulero, Victoriano

    2016-11-01

    In mammals, during the acute inflammatory response, the complex interrelationship and cross-talk among histamine and the immune system has been fairly well characterized. There is a substantial body of information on its structure, metabolism, receptors, signal transduction, physiologic and pathologic effects. However, for early vertebrates, there is little such knowledge. In the case of teleost fish, this lack of knowledge has been due to the widely held belief that histamine is not present in this phylogenetic group. However, it has been recently demonstrated, that granules of mast cells in perciforms contain biologically active histamine. More importantly, the inflammatory response was clearly demonstrated to be regulated by the direct action of histamine on professional phagocytes. Nevertheless, the molecular basis and exact role of this biogenic amine in perciforms is still a matter of speculation. Therefore, this review intends to summarize recent experimental evidence regarding fish mast cells and correlate the same with their mammalian counterparts to establish the possible role of histamine in the fish intestinal inflammatory response. Copyright © 2016 Elsevier Ltd. All rights reserved.

  4. The mitochondrial genome of the pufferfish, Fugu rubripes, and ordinal teleostean relationships.

    PubMed

    Elmerot, Christian; Arnason, Ulfur; Gojobori, Takashi; Janke, Axel

    2002-08-07

    The small nuclear genome of the pufferfish, Fugu rubripes (order Tetraodontiformes), makes this species highly interesting for genome research. In order to establish the phylogenetic position of the Tetraodontiformes relative to other teleostean orders that might also have a reduced nuclear genome size, we have sequenced the mitochondrial (mt) genome of the pufferfish. The gene order, nucleotide composition and evolutionary rate of the mt genome of the fugu correspond to those of other teleosts. This suggests that the evolution of this genome has not been affected by the processes that led to the dramatic reduction of the size of the nuclear genome of the fugu. The phylogenetic analyses, which were based on the concatenated amino acid sequences of twelve protein-coding mt genes, placed the fugu among the percomorphs. The affinities between the Tetraodontiformes and either the Perciformes or the Zeiformes were limited, however. The common notion of a separate euteleostean clade remained unsupported. The analyses did not support the traditional systematic understanding that the Clupeiformes constitute a basal teleostean lineage. In addition the findings strongly suggest that three teleostean orders, the Perciformes, Zeiformes and Scorpaeniformes, are paraphyletic.

  5. Evidence for meiotic drive as an explanation for karyotype changes in fishes.

    PubMed

    Molina, Wagner Franco; Martinez, Pablo A; Bertollo, Luiz Antônio C; Bidau, Claudio Juan

    2014-06-01

    The process of preferential chromosome segregation during meiosis has been suggested to be responsible for the predominance of certain chromosome types in the karyotypes of mammals, birds and insects. We developed an extensive analysis of the fixation of mono- or bibrachial chromosomes in the karyotypes of the large Actinopterygii fish group, a key link in the evolution of terrestrial vertebrates, in order to investigate the generality of meiotic drive in determining karyotypic macrotrends. Unlike mammals, fishes have markedly undergone several types of preferential chromosomal rearrangements throughout evolution. Data from the analyzed orders indicate a prevalence of karyotypes with few (<33%) or many (>66%) acrocentric chromosomes and a low number of karyotypes with balanced numbers of mono- and bi-brachial elements. Parallel trends towards a higher number of karyotypes with prevalence of monobrachial chromosomes occurred in phylogenetically close orders (e.g. Perciformes and Tetraodontiformes, and in the order Mugiliformes) and in clades with prevalence of bibrachial elements (e.g. Characiformes, Gymnotiformes, Siluriformes, and Cypriniformes). Some orders where fewer species were available for study, such as Atheriniformes and Anguilliformes, showed karyotype assemblages where both trends were present. Our results strongly suggest a primary role of meiotic drive in karyotypic evolution as indicated by the accumulation of monobrachial chromosomes in Perciformes and Cypriniformes, or bibrachial chromosomes in Siluriformes and Characiformes. Further examinations of the interaction between life history traits, environmental characteristics, and the fixation of chromosomal rearrangements would be exceedingly valuable.

  6. First description of a Pliocene ichthyofauna from Central Africa (site KL2, Kolle area, Eastern Djurab, Chad): What do we learn?

    NASA Astrophysics Data System (ADS)

    Otero, Olga; Pinton, Aurélie; Mackaye, Hassan Taisso; Likius, Andossa; Vignaud, Patrick; Brunet, Michel

    2009-06-01

    This is the first extensive study of a freshwater fish fauna from a Pliocene site in Central Africa, based on fossils collected at the KL2 site in the fossiliferous area of Kolle (Lower Pliocene, Chad). A relatively high fish diversity is revealed, confirming the presence of 19 taxa: Polypteriformes, Polypteridae ( Polypterus sp.); Osteoglossiformes, Osteoglossidae ( Heterotis sp.), Mormyriformes, Gymnarchidae ( Gymnarchus sp. cf. niloticus); Cypriniformes, Cyprinidae ( Labeo sp.); Characiformes, Alestidae ( Hydrocynus; Alestinae type Alestes/ Brycinus; Sindacharax sp. cf. deserti, Sindacharax sp.), Distichodontidae ( Distichodus sp.); Siluriformes, Ariidae (cf. Calarius), ?Bagridae (cf. Bagrus), Claroteidae (cf. Clarotes), Mochokidae ( Synodontis sp.), Clariidae ( Clarias sp. or Heterobranchus sp.); Perciformes family indet. ( Semlikiichthys sp. cf. darsao), Latidae ( Lates sp. cf. niloticus), Cichlidae indet., and Perciformes indet.; Tetraodontiformes Tetraodontidae ( Tetraodon sp.). The aquatic environment corresponding to the fossil fish assemblage might be a floodplain crossed by well-oxygenated open waters. Compared with a contemporaneous East African region, the mid-Pliocene Chadian fish diversity reveals a certain endemicity, while connections between the Niger and the Chadian basin are suspected because of the presence of a freshwater ariid fish in Kolle.

  7. Immunocytochemical applications of specific antisera raised against synthetic fragment peptides of mummichog GtH subunits: examining seasonal variations of gonadotrophs (FSH cells and LH cells) in the mummichog and applications to other acanthopterygian fishes.

    PubMed

    Shimizu, Akio; Tanaka, Hideki; Kagawa, Hirohiko

    2003-06-01

    Two distinct types of gonadotrophs, FSH (GtH I) cells and LH (GtH II) cells, were immunocytochemically identified from mummichog (Fundulus heteroclitus; Cyprinodontiformes, Acanthopterygii) pituitary using antisera raised against synthetic fragment peptides of FSHbeta and LHbeta. Both cell types were abundant during the spawning period (spring and early summer) and decreased in number during the post-spawning immature period. The number of FSH cells increased again during the early phases of gonadal development (cortical alveoli accumulation in the oocytes and basal spermatogenesis) in early winter, whereas the number of LH cells did not. Only FSH cells were abundant during the latter phases of gonadal development (vitellogenesis and active spermatogenesis) in early spring. These observations suggest that both GtHs have important yet different roles for reproduction in this species. Antisera against the conservative region of the FSHbeta and the LHbeta subunits immunostained FSH cells and LH cells, respectively, also in red seabream (Pagrus major; Perciformes, Acanthopterygii) and small mouth bass (Micropterus dolomieu; Perciformes, Acanthopterygii), suggesting the possibility of their use for other acanthopterygian fishes.

  8. Chromosomal distribution of two multigene families and the unusual occurrence of an X1X1X2X2/X1X2Y sex chromosome system in the dolphinfish (Coryphaenidae): an evolutionary perspective.

    PubMed

    Soares, R X; Bertollo, L A C; Cioffi, M B; Costa, G W W F; F Molina, W

    2014-04-03

    Dolphinfishes (Coryphaenidae) are pelagic predators distributed throughout all tropical and subtropical oceans and are very important for commercial, traditional, and sport fishing. This small family contains the Coryphaena hippurus and Coryphaena equiselis species whose chromosomal aspects remain unknown, despite recent advances in cytogenetic data assimilation for Perciformes. In this study, both species were cytogenetically analyzed using different staining techniques (C-, Ag-, and CMA3 banding) and fluorescence in situ hybridization, to detect 18S rDNA and 5S rDNA. C. hippurus females exhibit 2n = 48 chromosomes, with 2m+4sm+42a (NF = 54). In C. equiselis, where both sexes could be analyzed, females displayed 2n = 48 chromosomes (2m+6sm+40a) and males exhibited 2n = 47 chromosomes (3m+6sm+38a) (NF = 56), indicating the presence of X1X1X2X2/X1X2Y multiple sex chromosomes. Sex-chromosome systems are rare in Perciformes, with this study demonstrating the first occurrence in a marine pelagic species. It remains unknown as to whether this system extends to other populations; however, these data are important with respect to evolutionary, phylogenetic, and speciation issues, as well as for elucidating the genesis of this unique sex system.

  9. An Intermediate in the evolution of superfast sonic muscles

    PubMed Central

    2011-01-01

    Background Intermediate forms in the evolution of new adaptations such as transitions from water to land and the evolution of flight are often poorly understood. Similarly, the evolution of superfast sonic muscles in fishes, often considered the fastest muscles in vertebrates, has been a mystery because slow bladder movement does not generate sound. Slow muscles that stretch the swimbladder and then produce sound during recoil have recently been discovered in ophidiiform fishes. Here we describe the disturbance call (produced when fish are held) and sonic mechanism in an unrelated perciform pearl perch (Glaucosomatidae) that represents an intermediate condition in the evolution of super-fast sonic muscles. Results The pearl perch disturbance call is a two-part sound produced by a fast sonic muscle that rapidly stretches the bladder and an antagonistic tendon-smooth muscle combination (part 1) causing the tendon and bladder to snap back (part 2) generating a higher-frequency and greater-amplitude pulse. The smooth muscle is confirmed by electron microscopy and protein analysis. To our knowledge smooth muscle attachment to a tendon is unknown in animals. Conclusion The pearl perch, an advanced perciform teleost unrelated to ophidiiform fishes, uses a slow type mechanism to produce the major portion of the sound pulse during recoil, but the swimbladder is stretched by a fast muscle. Similarities between the two unrelated lineages, suggest independent and convergent evolution of sonic muscles and indicate intermediate forms in the evolution of superfast muscles. PMID:22126599

  10. Host-parasite relationships of Rhabdochona kidderi Pearse, 1936 (Nematoda: Rhabdochonidae) in fishes of the Lacantún River in the Lacandon rain forest of Chiapas State, southern Mexico, with a key to Mexican species of Rhabdochona Railliet, 1916.

    PubMed

    Moravec, František; Salgado-Maldonado, Guillermo; González-Solís, David; Caspeta-Mandujano, Juan M

    2012-05-01

    For the first time, the nematode Rhabdochona kidderi kidderi Pearse, 1936 (Rhabdochonidae) was recorded from fishes of the Lacantún River (Usumacinta River basin) in the Lacandon rain forest, Chiapas State, southern Mexico. Amphilophus nourissati (Allgayer) and Theraps irregularis Günther (both Perciformes: Cichlidae) were found to be the only definitive hosts in the locality, whereas Eugerres mexicanus (Steindachner) (Perciformes: Gerreidae), Ariopsis sp., Cathorops aguadulce (Meek) and Potomarius nelsoni (Evermann & Goldsborough) (all Siluriformes: Ariidae), Ictalurus furcatus (Valenciennes) (Siluriformes: Ictaluridae) and Strongylura hubbsi Collette (Beloniformes: Belonidae) all harboured the nematode's fourth-stage larva and only served as paratenic hosts. All these fish species represent new host records for this parasite. The morphology of both adults and larvae was studied in detail by light and scanning electron microscopy, and some conspecific museum specimens from three other host species were also examined for comparison. Rhabdochona ictaluri Aguilar-Aguilar, Rosas-Valdez & Pérez-Ponce de León, 2010 is considered here to be a junior synonym of R. kidderi kidderi. A high degree of the variability of some morphological and biometrical features (deirid shape, left spicule length) and an unusually wide range of hosts suggest that R. kidderi may represent a species complex, but further studies are necessary in this respect. A key to Rhabdochona species and subspecies occurring in Mexico is provided.

  11. Histamine is stored in mast cells of most evolutionarily advanced fish and regulates the fish inflammatory response

    PubMed Central

    Mulero, Iván; Sepulcre, M. Pilar; Meseguer, José; García-Ayala, Alfonsa; Mulero, Victoriano

    2007-01-01

    Mast cells are important as initiators and effectors of innate immunity and regulate the adaptive immune responses. They have been described in all classes of vertebrates and seem to be morphologically and functionally similar. However, early studies had shown that fish and amphibian mast cells were devoid of histamine. In this study, we take a fresh look at the evolution of histamine and find that the mast cells of fish belonging to the Perciformes order, the largest and most evolutionarily advanced order of teleosts, are armed with histamine. More importantly, histamine is biologically active in these fish where it is able to regulate the inflammatory response by acting on professional phagocytes. In addition, the actions of histamine in these immune cells seem to be mediated through the engagement of H1 and H2 receptors, which, together with the H3 receptor, are well conserved in bony fish. We propose that the storage of histamine in vertebrate mast cells and its use as an inflammatory messenger was established in primitive reptiles (Lepidosauria) ≈276 million years ago. This same feature seems to have developed independently in Perciform fish much more recently in the Lower Eocene, between 55 and 45 million years ago, a short period during which the great majority of Percomorph families appeared. PMID:18042725

  12. Convergent Aspects of Acoustic Communication in Darters, Sculpins, and Gobies.

    PubMed

    Zeyl, Jeffrey N; Malavasi, Stefano; Holt, Daniel E; Noel, Patricia; Lugli, Marco; Johnston, Carol E

    2016-01-01

    Darters (Perciformes, Percidae), sculpins (Perciformes, Cottidae), and gobioids (Gobiiformes, Gobioidei) exhibit convergent life history traits, including a benthic lifestyle and a cavity nesting spawning mode. Soniferous species within these taxa produce pulsed and/or tonal sounds with peak frequencies below 200 Hz (with some exceptions), primarily in agonistic and/or reproductive contexts. The reduced or absent swim bladders found in these taxa limit or prevent both hearing enhancement via pressure sensitivity and acoustic amplification of the contracting sonic muscles, which are associated with the skull and pectoral girdle. While such anatomies constrain communication to low frequency channels, optimization of the S/N (signal-to-noise) ratio in low frequency channels is evident for some gobies, as measured by habitat soundscape frequency windows, nest cavity sound amplification, and audiograms. Similar S/N considerations are applicable to many darter and sculpin systems. This chapter reviews the currently documented diversity of sound production in darters, sculpins, and gobioids within a phylogenetic context, examines the efficacy of signal transmission from senders to receivers (sound production mechanisms, audiograms, and masking challenges), and evaluates the potential functional significance of sound attributes in relation to territorial and reproductive behaviours.

  13. Five new species of philometrid nematodes (Philometridae) from marine fishes off Java, Indonesia.

    PubMed

    Moravec, Frantisek; Walter, Thorsten; Yuniar, Asri Trisnani

    2012-06-01

    Based on light and scanning electron microscopical studies, the following five species of the Philometridae (Nematoda: Dracunculoidea) are described from female specimens collected from marine fishes off the southwestern coast of Java, Indonesia: Philometra lobotidis sp. n. from the abdominal cavity of the Atlantic tripletail Lobotes surinamensis (Bloch) (Lobotidae, Perciformes); Philometra javaensis sp. n. from the abdominal cavity of the immaculate puffer Arothron immaculatus (Bloch et Schneider) (Tetraodontidae, Tetraodontiformes); Philometra psettoditis sp. n. from the musculature of the Indian spiny turbot Psettodes erumei (Bloch et Schneider) (Psettodidae, Pleuronectiformes); Philometroides indonesiensis sp. n. from the musculature of the hound needlefish Tylosurus crocodilus crocodilus (Péron et Lesueur) (Belonidae, Beloniformes); and Philometroides trichiuri sp. n. from the dorsal fin of the largehead hairtail Trichiurus lepturus Linnaeus (type host) and the savalai hairtail Lepturacanthus savala (Cuvier) (both Trichiuridae, Perciformes). All these new species are distinguished from their congeners parasitizing marine fishes by morphological (mainly the shape and structure of the cephalic and caudal ends and of the oesophagus) and biometrical features. Besides previously known Philometra pellucida (Jägerskiöld, 1893) and Philometra ocularis Moravec, Ogawa, Suzuki, Miyazaki et Donai, 2002, they are the only nominal philometrid species recorded from Indonesian waters.

  14. Muscular system in the pacific bluefin tuna Thunnus orientalis (Teleostei: Scombridae).

    PubMed

    Nakae, Masanori; Sasaki, Kunio; Shinohara, Gento; Okada, Tokihiko; Matsuura, Keiichi

    2014-02-01

    The muscular system in the Pacific bluefin tuna Thunnus orientalis is studied in detail. For the first time, a complete description of the muscular anatomy of a thunnid is provided here. Eighty-two elements including subdivisions of components of the muscular system are identified. This is less than found in a basal perciform and two other investigated scombrid species, owing mainly to the absence or fusion of pectoral, pelvic and caudal fin muscles. The absence of elements of the basal perciform pattern was most prominent in the caudal fin, which includes only the flexor dorsalis, flexor ventralis, hypochordal longitudinalis, and interradialis. In the caudal fin, the medial fan-shaped ray was identified as the first dorsal ray, judging from myological and neuroanatomical characters. The highly developed gill filament muscles in Thunnus orientalis and sheet-like rectus communis control gill ventilation. Long body muscle tendons reduce the metabolic energy needed during rapid and continuous swimming. These characters are interpreted as adaptations in the context of the oceanic life style of the species.

  15. Multiple vitellogenins and product yolk proteins in striped bass, Morone saxatilis: molecular characterization and processing during oocyte growth and maturation.

    PubMed

    Williams, V N; Reading, B J; Hiramatsu, N; Amano, H; Glassbrook, N; Hara, A; Sullivan, C V

    2014-04-01

    The multiple vitellogenin (Vtg) system of striped bass, a perciform species spawning nearly neutrally buoyant eggs in freshwater, was investigated. Vitellogenin cDNA cloning, Western blotting of yolk proteins (YPs) using Vtg and YP type-specific antisera, and tandem mass spectrometry (MS/MS) of the YPs revealed the complex mechanisms of yolk formation and maturation in this species. It was discovered that striped bass possesses a tripartite Vtg system (VtgAa, VtgAb, and VtgC) in which all three forms of Vtg make a substantial contribution to the yolk. The production of Vtg-derived YPs is generally similar to that described for other perciforms. However, novel amino-terminal labeling of oocyte YPs prior to MS/MS identified multiple alternative sites for cleavage of these proteins from their parent Vtg, revealing a YP mixture far more complex than reported previously. This approach also revealed that the major YP product of each form of striped bass Vtg, lipovitellin heavy chain (LvH), undergoes limited degradation to smaller polypeptides during oocyte maturation, unlike the case in marine fishes spawning buoyant eggs in which LvHAa undergoes extensive proteolysis to osmotically active free amino acids. These differences likely reflect the lesser need for hydration of pelagic eggs spawned in freshwater. The detailed characterization of Vtgs and their proteolytic fate(s) during oocyte growth and maturation establishes striped bass as a freshwater model for investigating teleost multiple Vtg systems.

  16. Coming out of the starting blocks: extended lag time rearranges genetic diversity in introduced marine fishes of Hawai'i.

    PubMed

    Gaither, Michelle R; Toonen, Robert J; Bowen, Brian W

    2012-10-07

    Biological invasions with known histories are rare, especially in the sea, and empirical studies of the genetic consequences are even rarer. Fifty-five years ago, the state of Hawai'i began a remarkable, if unintentional, 'experiment' with the introduction of three reef fishes, Lutjanus fulvus, Cephalopholis argus and Lutjanus kasmira. All have since expanded from the initial introduction of 2204 to 3163 individuals; however, historical records show that initially L. fulvus remained scarce, C. argus had modest population expansion and L. kasmira experienced rapid population growth. The consequences of differential population growth rates are apparent in F-statistics: Hawaiian L. fulvus demonstrate strong and significant haplotype frequency shifts from the founder location (F(ST) = 0.449), C. argus shows low but significant differentiation (F(ST) = 0.066) and L. kasmira is nearly identical to the founder location (F(ST) = 0.008). All three species had higher mtDNA diversity in the introduced range, which can be explained by multiple sources for L. fulvus and L. kasmira, but not for C. argus. We conclude that lag time before population expansion, in conjunction with genetic drift, has defined the genetic architecture of these three species in the introduced range.

  17. Coming out of the starting blocks: extended lag time rearranges genetic diversity in introduced marine fishes of Hawai‘i

    PubMed Central

    Gaither, Michelle R.; Toonen, Robert J.; Bowen, Brian W.

    2012-01-01

    Biological invasions with known histories are rare, especially in the sea, and empirical studies of the genetic consequences are even rarer. Fifty-five years ago, the state of Hawai‘i began a remarkable, if unintentional, ‘experiment’ with the introduction of three reef fishes, Lutjanus fulvus, Cephalopholis argus and Lutjanus kasmira. All have since expanded from the initial introduction of 2204 to 3163 individuals; however, historical records show that initially L. fulvus remained scarce, C. argus had modest population expansion and L. kasmira experienced rapid population growth. The consequences of differential population growth rates are apparent in F-statistics: Hawaiian L. fulvus demonstrate strong and significant haplotype frequency shifts from the founder location (FST = 0.449), C. argus shows low but significant differentiation (FST = 0.066) and L. kasmira is nearly identical to the founder location (FST = 0.008). All three species had higher mtDNA diversity in the introduced range, which can be explained by multiple sources for L. fulvus and L. kasmira, but not for C. argus. We conclude that lag time before population expansion, in conjunction with genetic drift, has defined the genetic architecture of these three species in the introduced range. PMID:22874747

  18. [Risk factors of ciguatera in the French West Indies in Saint-Barthélémy, Saint-Martin and Anguilla].

    PubMed

    Bourdeau, P; Bagnis, R

    1989-01-01

    An epidemiological study on ciguatera fish poisoning in the French West Indies (St-Barthelemy, St-Martin and Anguilla) was conducted during the years 1985-1986. The investigation on intoxications shows a non seasonal significant prevalence. Though it was difficult to list the cases, the morbidity seems to vary between 7 and 30 per thousand. A study of fish toxicity was realised; 46 different species were tested (individually or by pools) by the mosquito bio-test. Observations of intoxications give the following results: High risk species: Caranx bartholomaei, C. lugubris, Seriola dumerili, Lutjanus apodus, L. jocu, Pristipomoides macrophtalmus, Gymnothorax funebris, G. moringa, Scomberomorus cavalla, S. regalis, Mycteroperca venenosa, M. tigris, Epinephelus morio, Sphyraena barracuda. Intermediate species: Caranx latus, C. ruber, Lachnolaimus maximus, Lutjanus analis, L. buccanella, L. griseus, Malacanthus plumieri, Scomberomorus maculatus. Low risk species: Balistes vetula, Alectis ciliaris, Haemulon album, Bodianus rufus, Halichoeres radiatus, Priacanthus arenatus, Alphestes afer. Many species are involved in the toxic food chain. New ones have been identified, but it is difficult to determine the toxic level range. A cartography is presented but no place is free of risk. A research of Gambierdiscus toxicus, the causal agent, on algal surface from dead corals was conducted around St-Barthelemy and St-Martin. The dinoflagellate is found in low or medium populations all around the islands with no difference between North and South. There is a maximal activity during the spring. A model of the epidemiology of the ciguatera in the area is proposed.

  19. Growth and secondary production of an eventual reef fish during mangrove residency

    NASA Astrophysics Data System (ADS)

    Faunce, Craig H.; Serafy, Joseph E.

    2008-08-01

    Hierarchical data frameworks have been proposed to determine the relative value of fish habitats. Although conceptually accurate, judgments as to which habitat is more "essential" are limited by a lack of available information. This work provides much needed growth and production rates for gray snapper ( Lutjanus griseus), an ontogenetic habitat shifting reef fish, during mangrove residency. Ages were estimated using modal progression analysis of length-frequency distributions coupled with a biologically-realistic spawning date. Growth estimates place age-1 L. griseus at 155 mm (TL), age-2 at 243, and age-3 at 302 mm. These size-at-age estimates are consistent with otolith-based studies and are a natural extension of linear growth models for early juveniles. Lutjanus griseus primarily use southeastern Florida mangrove shoreline habitats for approximately 2 years between 0.86 and 2.84 years of age. Corresponding production:biomass ratios for these cohorts were 1.51 and 0.90. While these values are similar to other estimates for fishes inhabiting vegetated coastal wetlands in North America, they are much less than new estimates from a Bahamian mangrove creek system. These new data highlight important differences between the habitat value of mangrove shorelines of continental (low-relief) and island (high-relief) systems.

  20. Phylogenetic placement of enigmatic percomorph families (Teleostei: Percomorphaceae).

    PubMed

    Sanciangco, Millicent D; Carpenter, Kent E; Betancur-R, Ricardo

    2016-01-01

    Percomorphs are a large and diverse group of spiny-finned fishes that have come to be known as the "bush at the top" due to their persistent lack of phylogenetic resolution. Recently, the broader Euteleost Tree of Life project (EToL) inferred a well-supported phylogenetic hypothesis that groups the diversity of percomorphs into nine well-supported series (supraordinal groups): Ophidiaria, Batrachoidaria, Gobiaria, Syngnatharia, Pelagiaria, Anabantaria, Carangaria, Ovalentaria, and Eupercaria. The EToL also provided, for the first time, a monophyletic definition of Perciformes - the largest order of vertebrates. Despite significant progress made in accommodating the diversity of percomorph taxa into major clades, some 62 families (most previously placed in "Perciformes", as traditionally defined) were not examined by the EToL. Here, we provide evidence for the phylogenetic affinities of 10 of those 62 families, seven of which have largely remained enigmatic. This expanded taxonomic sampling also provides further support for the nine EToL supraordinal series. We examined sequences from 21 genes previously used by the EToL and added two fast-evolving mitochondrial markers in an attempt to increase resolution within the rapid percomorph radiations. We restricted the taxonomic sampling to 1229 percomorph species, including expanded sampling from recent studies. Results of maximum likelihood analysis revealed that bathyclupeids (Bathyclupeidae), galjoen fishes (Dichistiidae), kelpfishes (Chironemidae), marblefishes (Aplodactylidae), trumpeters (Latridae), barbeled grunters (Hapalogenyidae), slopefishes (Symphysanodontidae), and picarel porgies (formerly Centracanthidae) are members of the series Eupercaria ("new bush at the top"). The picarel porgies and porgies (Sparidae) are now placed in the same family (Sparidae). Our analyses suggest a close affinity between the orders Spariformes (including Lethrinidae, Nemipteridae and Sparidae) and Lobotiformes (including the

  1. Primordial germ cell migration in the yellowtail kingfish (Seriola lalandi) and identification of stromal cell-derived factor 1.

    PubMed

    Fernández, J A; Bubner, E J; Takeuchi, Y; Yoshizaki, G; Wang, T; Cummins, S F; Elizur, A

    2015-03-01

    Primordial germ cells (PGCs) are progenitors of the germ cell lineage, giving rise to either spermatogonia or oogonia after the completion of gonadal differentiation. Currently, there is little information on the mechanism of PGCs migration leading to the formation of the primordial gonad in perciform fish. Yellowtail kingfish (Seriola lalandi) (YTK) (order Perciforms) inhabit tropical and temperate waters in the southern hemisphere. Fundamental details into the molecular basis of larval development in this species can be easily studied in Australia, as they are commercially cultured and readily available. In this study, histological analysis of YTK larvae revealed critical time points for the migration of PGCs to the genital ridge, resulting in the subsequent development of the primordial gonad. In YTK larvae at 3, 5, 7 and 10 days post hatch (DPH), PGCs were not yet enclosed by somatic cells, indicating the primordial gonad had not yet started to form. While at 15, 18 and 20 DPH PGCs had already settled at the genital ridge and started to become enclosed by somatic cells indicating the primordial gonad had started to develop. A higher number of PGCs were observed in the larvae at 15 and 18 DPH indicating PGCs proliferation, which corresponds with them becoming enclosed by the somatic cells. Directional migration of PGCs toward the genital ridge is a critical event in the subsequent development of a gonad. In zebrafish, mouse and chicken, stromal-cell derived factor (SDF1) signalling is one of the key molecules for PGC migration. We subsequently isolated from YTK the SDF1 (Slal-SDF1) gene, which encodes for a 98-residue precursor protein with a signal peptide at the N-terminus. There is spatial conservation between fish species of four cysteine residues at positions C9, C11, C34 and C49, expected to form disulphide bonds and stabilize the SDF structure. In YTK, Slal-SDF1 gene expression analyses shows that this gene is expressed in larvae from 1 to 22 DPH and

  2. [The benthic fauna of Sabancuy Estuary, Campeche, Mexico].

    PubMed

    González Solís, A; Torruco Gómez, D

    2001-03-01

    The fish and invertebrates community structure in the Sabancuy estuary was analyzed in two seasons and 14 sampling stations (13 along the estuary and one in the marine adjacent coast). No significant differences were found between seasons. The environmental frame defines two zones within the estuary, the first extends from the access highway to Sabancuy town until the Pujo mouth in the west; the second from the bridge to the estuary head in the east. The most abundant invertebrates were mollusks (51.8% of the total), in biomass the crustaceans dominated. The fish included 21 families and 33 species; the most abundant were Gerridae, Scianidae, Sparidae, Lutjanidae and Ciprinodontidae. The highest diversities of both communities correspond to the central part of the estuary. These communities include three sections with notable differences in faunal distribution: one is influenced by the exit to Terminos lagoon, the secondary in the estuary head and a third is in a transition zone defined by the proximity of the town access bridge. The ecological organization suggests a strong division caused by the bridge, both sides are scarce in habitats and nutrient resources and this is reflected in the low species counts.

  3. Deep-reef fish assemblages of the Great Barrier Reef shelf-break (Australia).

    PubMed

    Sih, Tiffany L; Cappo, Mike; Kingsford, Michael

    2017-09-07

    Tropical mesophotic and sub-mesophotic fish ecology is poorly understood despite increasing vulnerability of deeper fish assemblages. Worldwide there is greater fishing pressure on continental shelf-breaks and the effects of disturbances on deeper fish species have not yet been assessed. Difficult to access, deeper reefs host undocumented fish diversity and abundance. Baited Remote Underwater Video Stations (BRUVS) with lights were used to sample deeper habitats (54-260 m), in the Great Barrier Reef (GBR), Australia. Here we describe fish biodiversity, relative abundance and richness, assessing the prediction that depth would drive assemblage structure in the GBR. Distinct groups of fishes were found with depth whilst overall richness and abundance decreased steeply between 100 and 260 m. Commercially-valuable Lutjanidae species from Pristipomoides and Etelis genera, were absent from shallower depths. Few fish species overlapped between adjacent depth strata, indicating unique assemblages with depth. We also detected new location records and potential new species records. The high biodiversity of fish found in shelf-break environments is poorly appreciated and depth is a strong predictor of assemblage composition. This may pose a challenge for managers of commercial fisheries as distinct depth ranges of taxa may translate to more readily targeted habitats, and therefore, an inherent vulnerability to exploitation.

  4. [Evaluation of Antilles fish ciguatoxicity by mouse and chick bioassays].

    PubMed

    Pottier, I; Vernoux, J P

    2003-03-01

    Ciguatera is a common seafood poisoning in Western Atlantic and French West Indies. Ciguatera fish poisoning in the Caribbean is a public health problem. A toxicological study was carried out on 178 Caribbean fish specimens (26 species) captured off Guadeloupe and Saint Barthelemy between 1993 and 1999. The mouse bioassay and the chick feeding test were used to control fish edibility. Ciguatoxins presence was assumed when symptomatology was typical of ciguatera in mouse and chick. Fishes were classified in three groups: non toxic fish (edible), low toxic fish (not edible) and toxic fish (not edible). 75% of fishes were non toxic. Toxic fish specimens belonged to four families of high trophic level carnivores: Carangidae, Lutjanidae, Serranidae et Sphyraenidae. Percentages of toxic fishes to humans reached 55% for Caranx latus and 33% for Caranx bartholomaei and Caranx lugubris. Only a significant correlation between weight and toxicity was only found for C. latus and snappers. Small carnivorous groupers (Serranidae) were also toxic. Atoxic fish species were (a) pelagic fish (Coryphaena hippurus, Auxis thazard and Euthynnus pelamis), (b) invertebrates feeders (Malacanthus plumieri, Balistes vetula), (c) small high-risk fish or (d) fish of edible benthic fish families. Liver of four fishes (Mycteroperca venenosa, Caranx bartholomaei, Seriola rivoliana, Gymnothorax funebris) contained ciguatoxins at a significant level although their flesh was safe. This study confirms the usefulness of mouse and chick bioassays for sanitary control of fish.

  5. [Ichthyofauna of the Navidad Bay, Jalisco, México].

    PubMed

    Aguilar-Palomino, B; Pérez Reyes, C; Galván-Magaña, F; Abitía-Cardenas, L A

    2001-03-01

    A check-list and the relative abundance of the ichthyofauna from Bahia de Navidad, Jalisco, Mexico is presented. This poorly known area of the eastern Tropical Pacific ocean is characterized by its great fish diversity. A total of 245 fish species (163 genera, 72 families) were collected during the 1994-1995 annual cycle. Vouchers were deposited in Centro de Ecologia Costera, Universidad de Guadalajara. The most important families in number of species were Carangidae (20 species), Haemulidae (20), Serranidae (17), Sciaenidae (13) and Lutjanidae (8); 20 species represented 85% and 80% of relative abundance and biomass respectively. Microlepidotus brevipinnis, Caranx caninus, Caranx caballus, Kiphosus analogus and Caranx sexfasciatus, were the dominant species. Most fish species collected in Bahia de Navidad have zoogeographical affinity with the Panamic Province (65%), and in lesser proportion with the Eastern Pacific Ocean (19%), Circumtropical (7%), Mexican Province (5%), Indo-Pacific Ocean (3%) and Gulf of California (0.4%). The great ichthyological affinity between the study area and the southern Gulf of California suggests an extension of the of Gulf southern limit.

  6. [Ichthyoplankton from the pier area in Limón, Costa Rica].

    PubMed

    Dominici-Arosemena, A; Brugnoli-Olivera, E; Solano-Ulate, S; Molina-Ureña, H; Ramírez Coghi, A R

    2000-01-01

    Four sampling stations were set 200-500 m off Limon Port, Costa Rica, at an average depth of 20 m. From October 1996 to May 1997 horizontal subsuperficial trawls were done for 12 minutes at a speed of about 2 Km/h, with a 1,000 microns plancton net. A total of 28 samples were processed and 104 physical-chemistry parameter measurements were taken. Average salinity was 30.1 +/- 3.7 and dissolved oxygen 6.9 +/- 0.6 mg l-1 reflecting good aeration throughout the sampling period. This suggests good mixture and a highly dynamic hydrography. Temperature showed no drastic variations (28.0 +/- 1.7 degrees C), possibly because of the constant mixing with shallow water. The highest larval counts were for November and early January and include families common to reefs and estuaries (Balistidae, Lutjanidae) and to coastal areas (Centropomidae, Gerreidae, Haemulidae, Carangidae, Engraulidae, Hemiramphidae, and representative Pleuronectiformes) existing in a common area.

  7. Henneguya tunisiensis n. sp. (Myxosporea: Bivalvulida), a new gill parasite of Symphodus tinca (L.) (Teleostei: Labridae) off Tunisia.

    PubMed

    Bahri, Sihem; Marton, Szilvia; Marques, Adam; Eszterbauer, Edit

    2010-06-01

    Henneguya tunisiensis n. sp., a new myxosporean, is described from the gill-arches of the East Atlantic peacock wrasse Symphodus tinca (L.) collected from off the Kerkennah Islands, Tunisia. It is characterised by the presence of elongate white plasmodia of 1-1.5 x 1.5-2 mm in size. The mature spores are rounded in frontal view and have two identical polar capsules and two caudal appendages which taper considerably at the end. Both light and electron microscopical data show that this species differs in several morphological features from all previously described Henneguya spp. A molecular analysis, based on 18S rDNA sequence data, indicates that H. tunisiensis n. sp. is readily distinguishable from other myxozoan DNA sequences in GenBank. Phylogenetically, the new species is placed in the marine Henneguya clade, which is a sister group of marine Myxobolus spp. from perciform fishes in Tunisian waters.

  8. Novel evolutionary relationship among four fish model systems.

    PubMed

    Chen, Wei-Jen; Ortí, Guillermo; Meyer, Axel

    2004-09-01

    Knowledge of the correct phylogenetic relationships among animals is crucial for the valid interpretation of evolutionary trends in biology. Zebrafish, medaka, pufferfish and cichilds are fish models for development, genomics and comparative genetics studies, although their phylogenetic relationships have not been tested rigorously. The results of phylogenomic analysis based on 20 nuclear protein-coding genes confirmed the basal placement of zebrafish in the fish phylogeny but revealed an unexpected relationship among the other three species, contrary to traditionally held systematic views based on morphology. Our analyses show that medaka (Beloniformes) and cichlids (Perciformes) appear to be more closely related to each other than either of them is to pufferfish (Tetraodontiformes), suggesting that a re-interpretation of some findings in comparative biology might be required. In addition, phylogenomic analyses show that fish typically have more copies of nuclear genes than land vertebrates, supporting the fish-specific genome duplication hypothesis.

  9. Preliminary findings on the nucleus of large neurons in Lophius piscatorius L. (Osteichthyes, Lophiiformes).

    PubMed

    Benedetti, I; Mola, L

    1991-01-01

    Some Teleosts belonging to the orders Batrachoidiformes, Lophiiformes, Perciformes and Tetraodontiformes show a cluster of large neurons dorsally located at the boundary between the spinal cord and medulla oblongata. These elements have traditionally been grouped with the supramedullary neurons aligned in the dorso-medial region of the spinal cord of other teleosts (see Marini and Benedetti, in press). However, recent morphological and immunohistochemical studies have suggested that the neurons grouped in a cluster on the spinal cord of Lophius piscatorius may not be of the same nature as the supramedullary neurons aligned within the dorsal gray matter of the spinal cord (Benedetti and Mola, 1988; Mola, 1990). Over the course of these previous investigations, it seemed that the nucleus of the neurons in the cluster showed more abundant chromatin than other neurons in the dorsal spinal cord. This prompted a series of investigations on the nucleus of these neurons.

  10. Capillaria plectropomi n. sp. (Nematoda: Capillariidae), a new intestinal parasite of the leopard coral grouper Plectropomus leopardus (Serranidae) off New Caledonia

    PubMed Central

    Moravec, František; Justine, Jean-Lou

    2014-01-01

    A new nematode species, Capillaria plectropomi n. sp. (Capillariidae), is described from the intestine of the leopard coral grouper Plectropomus leopardus (Lacepède) from coral reefs off New Caledonia. The new species, belonging to the subgenus Neocapillaria Moravec, 1987, differs from other congeneric species of this subgenus from marine fishes mainly in the length (168–186 μm), shape and structure of the spicule. It is characterized, in the male, by the presence of two well-developed dorsolateral caudal lobes, a pair of lateral papillae, a heavily sclerotized spicule with many rough transverse grooves in the middle part, a spinose spicular sheath, and in the female, by eggs measuring 60–66 × 27 μm without protruding polar plugs. The buccal cavity contains a small finger-shaped stylet. Capillaria plectropomi n. sp. is the first known species of this genus parasitizing fishes of the perciform family Serranidae. PMID:25531932

  11. New records of species of Philometra (Nematoda: Philometridae) from marine fishes off New Caledonia, including P. cephalopholidis sp. n. from Cephalopholis sonnerati (Serranidae).

    PubMed

    Moravec, František; Justine, Jean-Lou

    2015-09-01

    Two different species of Philometra Costa, 1845 were collected from marine perciform fishes, the tomato hind Cephalopholis sonnerati (Valenciennes) (Serranidae) and the painted sweetlips Diagramma pictum (Thunberg) (Haemulidae), from off New Caledonia, South Pacific. Nematodes (only males) from the gonad of C. sonnerati represent a new taxon, P. cephalopholidis sp. n., which is mainly characterized by almost equally long spicules (length 186-228 μm), the shape and structure of the gubernaculum with a dorsally lamellate distal tip, and the structure of the caudal end. The nematodes (only gravid females) from abdominal tissues of D. pictum may represent an undescribed species, but, because of the absence of conspecific males, they could not be specifically identified. Philometra cephalopholidis is the sixth nominal species of this genus recorded from fishes off New Caledonia and the thirteenth species of these parasites described from fishes of the family Serranidae.

  12. Philometra spicarae sp. n. (Nematoda: Philometridae) from the abdominal cavity of the marine fish (picarel) Spicara smaris (Centracanthidae) off Sicily, Italy.

    PubMed

    Moravec, Frantisek; Gaglio, Gabriella; Giannetto, Salvatore; Marino, Fabio

    2010-07-01

    A new nematode species, Philometra spicarae sp. n. (Philometridae), is described from a gravid female found in the abdominal cavity of spicarel, Spicara smaris (Linnaeus) (Centracanthidae, Perciformes), from the Ionian Sea off Sicily, Italy. The new species differs from most other Philometra spp. parasitic in the abdominal cavity of marine fishes by the oesophagus without an anterior inflation, resembling thus only Philometra cheilopogoni Mordvinova, 1986 and Philometra justinei Moravec, Ternengo and Levron, 2006; these two species can be differentiated from P. spicarae by the relative length of the oesophagus to body length (0.6-0.8% vs. 6%) and some other features. From the gonad-infecting species Philometra filiformis (Stossich, 1896), a parasite of Pagellus erythrinus (Linnaeus), P. spicarae differs mainly in having a conspicuously long (1.4 mm) intestinal ligament. It is the first nominal philometrid species described from the fish of the family Centracanthidae.

  13. Dactylogyrids (Monogenoidea) parasitizing the gills of spinefoots (Teleostei: Siganidae): proposal of Glyphidohaptor n. gen., with two new species from the Great Barrier Reef, Australia, and G. plectocirra n. comb. from Ras Mohammed National Park, Egypt.

    PubMed

    Kritsky, Delane C; Galli, Paolo; Yang, Tingbao

    2007-02-01

    Nine species of Siganus (Perciformes: Siganidae) were examined for dactylogyrids (Monogenoidea) from the Red Sea, Egypt; the Great Barrier Reef, Australia; and the South China Sea, China. Species of Tetrancistrum were found on siganids from all 3 localities; Pseudohaliotrema spp. were restricted to siganids from the Great Barrier Reef; and species representing Glyphidohaptor n. gen. were found on siganids from the Red Sea and Great Barrier Reef. Siganus argenteus from the Red Sea and Siganus vulpinus from the Great Barrier Reef were negative for dactylogyrid parasites. Glyphidohaptor n. gen. is proposed for 3 species (2 species new to science) and the new species are described: Glyphidohaptor phractophallus n. sp. from Siganus fuscescens from the Great Barrier Reef; Glyphidohaptor sigani n. sp. from Siganus doliatus (type host), Siganus punctatus, Siganus corallinus, and Siganus lineatus from the Great Barrier Reef; and Glyphidohaptor plectocirra (Paperna, 1972) n. comb. (= Pseudohaliotrema plectocirra Paperna, 1972) from Siganus luridus and Siganus rivulatus from the Red Sea.

  14. First report on molecular evidence of Tylodelphys cerebralis (Diplostomulum cerebralis) Chakrabarti, 1968 (Digenea: Diplostomidae) from snakehead fish Channa punctata.

    PubMed

    Chaudhary, Anshu; Tripathi, Richa; Gupta, Shivi; Shanker Singh, Hridaya

    2017-06-01

    Freshwater snakehead fish, Channa punctata (Perciformes: Channidae) from Meerut (U.P.), India were screened for infection with metacercaria from the eye (vitreous humor) and brain (cranial cavity) and were analyzed by molecular methods using PCR and sequencing of the complete internal transcribed spacers (ITS1-5.8S-ITS2) of the ribosomal RNA gene. Recovered metacercariae from sites, vitreous humor and cranial cavity were previously diagnosed as Diplostomulum cerebralis Chakrabarti, 1968 on the basis of morphological characteristics. A combination of molecular methods in this study depicts that this is a species of genus Tylodelphys Diesing, 1850 which was misinterpreted as Diplostomulum. Hence, in this study, we validated the status of T. cerebralis (=D. cerebralis) after 49 years from its original description. The present work might contribute to expand our knowledge for identification, biodiversity and taxonomy of diplostomids in Indian fishes.

  15. Primary structures of sardaines Z1 and Z2, protamines isolated from striped bonito (Sarda orientalis).

    PubMed

    Okamoto, Y; Kuno, K; Motohiro, T; Nishi, N; Muta, E; Ota, S

    1992-02-01

    Striped bonito protamine, sardaine, was isolated from the sperm of striped bonito (Sarda orientalis) by extraction with sulfuric acid followed by ion-exchange chromatography. The preparation gave a single band upon polyacrylamide gel electrophoresis. Sardaine consists of 34 amino acid residues, and its sequence is: Pro-Arg-Arg-Arg-Arg-Arg-Ser(Ala)-Ser-Arg-Pro-Val-Arg-Arg-Arg-Arg-Arg-Tyr -Arg- Arg-Ser-Thr-Ala-Ala-Arg-Arg-Arg-Arg-Arg-Val-Val-Arg-Arg-Arg-Arg. At position 7, serine (sardaine Z1) is partially replaced by alanine (sardaine Z2). The ion spray mass spectrum shows that sardaines Z1 and Z2 have molecular masses of 4,612.49 and 4,596.09 Da, respectively. The sequence of sardaine Z1 is 100% identical with that of thynnine Z2 from tuna fish (both fish belong to Scombridae, Perciformes).

  16. Paraphilometroides nemipteri gen. et sp. n. (Nematoda: Philometridae) from the marine fish Nemipterus peronii (Valenciennes) from Malaysia.

    PubMed

    Moravec, F; Shaharom-Harrison, F

    1989-01-01

    A new nematode species, Paraphilometroides nemipteri sp. n. is