Safety for Your Child: Birth to 6 Months

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ELECTRONIC PULSE SCALING CIRCUITS

DOEpatents

Cooke-Yarborough, E.H.

1958-11-18

Electronic pulse scaling circults of the klnd comprlsing a serles of bi- stable elements connected ln sequence, usually in the form of a rlng so as to be cycllcally repetitive at the highest scallng factor, are described. The scaling circuit comprises a ring system of bi-stable elements each arranged on turn-off to cause, a succeeding element of the ring to be turned-on, and one being arranged on turn-off to cause a further element of the ring to be turned-on. In addition, separate means are provided for applying a turn-off pulse to all the elements simultaneously, and for resetting the elements to a starting condition at the end of each cycle.

The Star-forming Main Sequence of Dwarf Low Surface Brightness Galaxies

NASA Astrophysics Data System (ADS)

McGaugh, Stacy S.; Schombert, James M.; Lelli, Federico

2017-12-01

We explore the star-forming properties of late-type, low surface brightness (LSB) galaxies. The star-forming main sequence ({SFR}-{M}* ) of LSB dwarfs has a steep slope, indistinguishable from unity (1.04 ± 0.06). They form a distinct sequence from more massive spirals, which exhibit a shallower slope. The break occurs around {M}* ≈ {10}10 {M}⊙ , and can also be seen in the gas mass—stellar mass plane. The global Kennicutt-Schmidt law ({SFR}-{M}g) has a slope of 1.47 ± 0.11 without the break seen in the main sequence. There is an ample supply of gas in LSB galaxies, which have gas depletion times well in excess of a Hubble time, and often tens of Hubble times. Only ˜ 3 % of this cold gas needs be in the form of molecular gas to sustain the observed star formation. In analogy with the faint, long-lived stars of the lower stellar main sequence, it may be appropriate to consider the main sequence of star-forming galaxies to be defined by thriving dwarfs (with {M}* < {10}10 {M}⊙ ), while massive spirals (with {M}* > {10}10 {M}⊙ ) are weary giants that constitute more of a turn-off population.

On the determination of age and mass functions of stars in young open star clusters from the analysis of their luminosity functions

NASA Astrophysics Data System (ADS)

Piskunov, A. E.; Belikov, A. N.; Kharchenko, N. V.; Sagar, R.; Subramaniam, A.

2004-04-01

We construct the observed luminosity functions of the remote young open clusters NGC 2383, 2384, 4103, 4755, 7510 and Hogg 15 from CCD observations of them. The observed LFs are corrected for field star contamination determined with the help of a Galactic star count model. In the case of Hogg 15 and NGC 2383 we also consider the additional contamination from neighbouring clusters NGC 4609 and 2384, respectively. These corrections provide a realistic pattern of cluster LF in the vicinity of the main-sequence (MS) turn-on point and at fainter magnitudes reveal the so-called H-feature arising as a result of the transition of the pre-MS phase to the MS, which is dependent on the cluster age. The theoretical LFs are constructed representing a cluster population model with continuous star formation for a short time-scale and a power-law initial mass function (IMF), and these are fitted to the observed LF. As a result, we are able to determine for each cluster a set of parameters describing the cluster population (the age, duration of star formation, IMF slope and percentage of field star contamination). It is found that in spite of the non-monotonic behaviour of observed LFs, cluster IMFs can be described as power-law functions with slopes similar to Salpeter's value. The present main-sequence turn-on cluster ages are several times lower than those derived from the fitting of theoretical isochrones to the turn-off region of the upper main sequences.

The Multiple Stellar Populations in the Ancient LMC Globular Clusters Hodge 11 and NGC 2210

NASA Astrophysics Data System (ADS)

Chaboyer, Brian; Gilligan, Christina; Wagner-Kaiser, Rachel; Mackey, Dougal; Sarajedini, Ata; Cummings, Jeffrey; Grocholski, Aaron; Geisler, Doug; Cohen, Roger; Villanova, Sandro; Yang, Soung-Chul; Parisi, Celeste

2018-01-01

Hubble Space telescope images of the ancient LMC globular clusters Hodge 11 and NGC 2210 in the F336W, F606W and F814W filters were obtained between June 2016 and April 2017. These deep images has been analyzed with the Dolphot software package. High quality photometry has been obtained from three magnitudes brighter than the horizontal branch, to about four magnitudes fainter than the main sequence turn-off. Both clusters show an excess of red main sequence stars in the F336W filter, indicating that multiple stellar populations exist in both clusters. Hodge 11 shows irregularities in its horizontal branch morphology, which is indicative of the presence of an approximately 0.1 dex internal helium abundance spread.

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A white dwarf cooling age of 8 Gyr for NGC 6791 from physical separation processes.

PubMed

García-Berro, Enrique; Torres, Santiago; Althaus, Leandro G; Renedo, Isabel; Lorén-Aguilar, Pablo; Córsico, Alejandro H; Rohrmann, René D; Salaris, Maurizio; Isern, Jordi

2010-05-13

NGC 6791 is a well studied open cluster that it is so close to us that can be imaged down to very faint luminosities. The main-sequence turn-off age ( approximately 8 Gyr) and the age derived from the termination of the white dwarf cooling sequence ( approximately 6 Gyr) are very different. One possible explanation is that as white dwarfs cool, one of the ashes of helium burning, (22)Ne, sinks in the deep interior of these stars. At lower temperatures, white dwarfs are expected to crystallize and phase separation of the main constituents of the core of a typical white dwarf ((12)C and (16)O) is expected to occur. This sequence of events is expected to introduce long delays in the cooling times, but has not hitherto been proven. Here we report that, as theoretically anticipated, physical separation processes occur in the cores of white dwarfs, resolving the age discrepancy for NGC 6791.

DuOCam: A Two-Channel Camera for Simultaneous Photometric Observations of Stellar Clusters

NASA Astrophysics Data System (ADS)

Maier, Erin R.; Witt, Emily; Depoy, Darren L.; Schmidt, Luke M.

2017-01-01

We have designed the Dual Observation Camera (DuOCam), which uses commercial, off-the-shelf optics to perform simultaneous photometric observations of astronomical objects at red and blue wavelengths. Collected light enters DuOCam’s optical assembly, where it is collimated by a negative doublet lens. It is then separated by a 45 degree blue dichroic filter (transmission bandpass: 530 - 800 nm, reflection bandpass: 400 - 475 nm). Finally, the separated light is focused by two identical positive doublet lenses onto two independent charge-coupled devices (CCDs), the SBIG ST-8300M and the SBIG STF-8300M. This optical assembly converts the observing telescope to an f/11 system, which balances maximum field of view with optimum focus. DuOCam was commissioned on the McDonald Observatory 0.9m, f/13.5 telescope from July 21st - 24th, 2016. Observations of three globular and three open stellar clusters were carried out. The resulting data were used to construct R vs. B-R color magnitude diagrams for a selection of the observed clusters. The diagrams display the characteristic evolutionary track for a stellar cluster, including the main sequence and main sequence turn-off.

Very Low Mass Stars with Extremely Low Metallicity in the Milky Way's Halo

NASA Astrophysics Data System (ADS)

Aoki, Wako; Beers, Timothy C.; Takuma, Suda; Honda, Satoshi; Lee, Young Sun

2015-08-01

Large surveys and follow-up spectroscopic studies in the past few decades have been providing chemical abundance data for a growing number of very metal-poor ([Fe/H] <-2) stars. Most of them are red giants or main-sequence turn-off stars having masses near 0.8 solar masses. Lower mass stars with extremely low metallicity ([Fe/H] <-3) have yet to be well explored. Our high-resolution spectroscopic study for very metal-poor stars found with SDSS has identified four cool main-sequence stars with [Fe/H] <-2.5 among 137 objects (Aoki et al. 2013, AJ, 145, 13). The effective temperatures of these stars are 4500--5000 K, corresponding to a mass of around 0.5 solar masses. Our standard analysis of the high-resolution spectra based on 1D-LTE model atmospheres have obtained self-consistent chemical abundances for these objects, assuming small values of micro-turbulent velocities compared with giants and turn-off stars. The low temperature of the atmospheres of these objects enables us to measure their detailed chemical abundances. Interestingly, two of the four stars have extreme chemical abundance patterns: one has the largest excesses of heavy neutron-capture elements associated with the r-process abundance pattern known to date (Aoki et al. 2010, ApJL 723, L201), and the other exhibits low abundances of the alpha-elements and odd-Z elements, suggested to be the signatures of the yields of very massive stars ( >100 solar masses; Aoki et al. 2014, Science 345, 912). Although the sample size is still small, these results indicate the potential of very low-mass stars as probes to study the early stages of the Milky Way's halo formation.

Very Low-Mass Stars with Extremely Low Metallicity in the Milky Way's Halo

NASA Astrophysics Data System (ADS)

Aoki, Wako; Beers, Timothy C.; Suda, Takuma; Honda, Satoshi; Lee, Young Sun

2016-08-01

Large surveys and follow-up spectroscopic studies in the past few decades have been providing chemical abundance data for a growing number of very metal-poor ([Fe/H] <-2) stars. Most of them are red giants or main-sequence turn-off stars having masses near 0.8 solar masses. Lower mass stars with extremely low metallicity ([Fe/H] <-3) are yet to be explored. Our high-resolution spectroscopic study for very metal-poor stars found with SDSS has identified four cool main-sequence stars with [Fe/H] <-2.5 among 137 objects (Aoki et al. 2013). The effective temperatures of these stars are 4500-5000 K, corresponding to a mass of around 0.5 solar masses. Our standard analysis of the high-resolution spectra based on 1D-LTE model atmospheres has obtained self-consistent chemical abundances for these objects, assuming small values of micro-turbulent velocities compared with giants and turn-off stars. The low temperature of the atmospheres of these objects enables us to measure their detailed chemical abundances. Interestingly, two of the four stars have extreme chemical-abundance patterns: one has the largest excesses of heavy neutron-capture elements associated with the r-process abundance pattern known to date (Aoki et al. 2010), and the other exhibits low abundances of the α-elements and odd-Z elements, suggested to be signatures of the yields of very massive stars (> 100 solar masses; Aoki et al. 2014). Although the sample size is still small, these results indicate the potential of very low-mass stars as probes to study the early stages of the Milky Way's halo formation.

Working Parents

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... or go to the gym. Do some recreational reading. While family time is important and certain chores ...

Pediatric Ophthalmologist

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... with a vision screening exam or has difficulty reading or learning, or needs surgery or medical treatment ...

Friend or Foe?

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... best in him, rather than the worst. By reading up on the subject, discussing it with my ...

Disruptive Behavior Disorders

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Deployment and Children

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Health Screenings at School

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... others develop later. A child who has difficulty reading the blackboard may not know that she is ...

Stresses of Single Parenting

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... to enjoy your children. Spend quiet time playing, reading, working on arts-and-crafts projects, or just ...

Importance of Family Routines

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... child to sleep. These rituals can include storytelling, reading aloud, conversation, and songs. Try to avoid exciting ...

Preparing for Twins

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... challenges with twins. He also can suggest helpful reading material or refer you to organizations that help ...

The effects of the use of piezoelectric motors in a 1.5-Tesla high-field magnetic resonance imaging system (MRI).

PubMed

Wendt, O; Oellinger, J; Lüth, T C; Felix, R; Boenick, U

2000-01-01

This paper presents the results of an experimental investigation with two different rotatory piezomotors in a closed 1.5 Tesla high-field MRI. The focus of the investigation was on testing the functionality of these motors within the MRI and to determining the image interference they caused. To obtain a differentiated estimate of the interference the motors were tested in both the passive (turned off, i.e. without current flow) and active (turned on, i.e. with current flow) state during MRI scanning. Three different types of sequences were used for the test: Spin-Echo (SE), Gradient-Echo (GE) and Echo-Planar Imaging (EPI). A plastic container filled with a gadolinium-manganese solution was used for representation of the artefacts. The motors investigated were placed parallel to the container at predetermined distances during the experiment. The results show that the motors investigated suffered no functional limitations in the magnetic field of the MRI but, depending on the type of motor, the measurement distance and the state of the motor, the motors had different effects on the sequence images. A motor in the off-state placed immediately next to the object to be measured mainly causes artefacts because of its material properties. If, on the other hand, the piezomotor is in the on-state images with strong noise result when the motor is immediately next to the object being measured. The images regain their normal quality when the motor is approximately at a distance of 1 m from the object being investigated. Driving the motor inside the MRI, therefore, is only to be recommended during the pauses in scanning: this delivers artefact-free images if minimal, motor-specific distances are kept to. With regard to the three different types of sequences it was determined that the SE sequence was the least sensitive and the EPI sequence the most sensitive to disturbance. The GE sequence showed only minimal differences to the SE sequence with regard to signal-to-noise ratios. Since it requires considerably shorter scan-times it can be considered to be the most effective type of sequence under these conditions.

What Is a Pediatric Anesthesiologist?

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... and waiting rooms, which may have toys and reading materials for children. This helps create a comfortable ...

Language Development: 2 Year Olds

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... enrich his vocabulary and language skills by making reading a part of your everyday routine. At this ...

What Is a Pediatric Neurosurgeon?

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Developmental Milestones of Early Literacy

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Social Development: 1 Year Olds

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... re doing around the house. Whether you’re reading the paper, sweeping the floors, mowing the lawn, ...

Mass-losing red giants in open clusters

NASA Technical Reports Server (NTRS)

Jura, M.

1987-01-01

Mass-losing stars in open clusters with main-sequence turn-offs at intermediate mass have been searched for by using the IRAS data base. The absence of many strong 60 micron sources in open clusters implies that intermediate-mass stars lose much of their mass during an intense wind phase of rather short duration. For stars of about seven solar masses, this phase, if it exists at all, lasts for not much more than 100,000 yr. For stars of about four solar masses, the intense wind phase appears to last considerably less than 10 million yr; it may well last for less than a million yr.

Toilet Training Children with Special Needs

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... ten minutes at a time—keeping him company, reading to him, playing tapes of children’s music, and ...

Language Delays in Toddlers: Information for Parents

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... you to spend lots of time playing with, reading to, and talking with your infant or toddler. ...

What Is a Pediatric Sports Medicine Specialist?

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... Ribbon Commands Skip to main content Turn off Animations Turn on Animations Our Sponsors Log in | Register Menu Log in | ... in mind. They may have toys, videos, and reading materials for young people available. Pediatric sports medicine ...

Evacuation and Community Shelters

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... cleaning solution − Emergency preparedness kit − Clothing and bedding − Car keys If local officials haven’t advised an immediate evacuation, take these steps to protect your home before you leave: − Turn off electricity at the main fuse or breaker and turn ...

Power Supply Fault Tolerant Reliability Study

DTIC Science & Technology

1991-04-01

easier to design than for equivalent bipolar transistors. MCDONNELL DOUGLAS ELECTRONICS SYSTEMS COMPANY 9. Base circuitry should be designed to drive...SWITCHING REGULATORS (Ref. 28), SWITCHING AND LINEAR POWER SUPPLY DESIGN (Ref. 25) 6. Sequence the turn-off/turn-on logic in an orderly and controllable ...for equivalent bipolar transistors. MCDONNELL DOUGLAS ELECTRONICS SYSTEMS COMPANY 8. Base circuitry should be designed to drive the transistor into

Supraspinal metabotropic glutamate receptor subtype 8: a switch to turn off pain.

PubMed

Palazzo, Enza; de Novellis, Vito; Rossi, Francesco; Maione, Sabatino

2014-06-01

Glutamate is the main excitatory neurotransmitter in the central nervous system and as such controls the majority of synapses. Glutamatergic neurotransmission is mediated via ionotropic and metabotropic glutamate receptors (iGluRs and mGluRs). Signaling via mGluRs permits to finely tune, rather than turning on/off, the excitatory neurotransmission as the iGluRs do. Eight mGluRs (mGluR1-8) have been cloned so far, which have been divided into three groups based on sequence homology, pharmacological properties and second messenger signaling. mGluRs are widely expressed both on glia and neurons. On neurons they are located both at postsynaptic (group I) and presynaptic sites (group II and III). Group II and III mGluR stimulation reduces glutamate release, which can prove useful in pathological conditions characterized by elevated glutamatergic neurotransmission which include chronic pain. Indeed, mGluRs are widely distributed on pain neuraxis. The recent development of selective mGluR ligands has permitted investigating the individual role of each mGluR on pain control. The development of (S)-3,4-dicarboxyphenylglycine, a selective mGluR8 agonist, has revealed the mGluR8 role in inhibiting pain and its related affective consequences in chronic pain conditions. mGluR8 proved also to be overexpressed in pain controlling areas during pathological pain guaranteeing the availability of a switch for turning off abnormal pain. Thus, mGluR8 corresponds to an ideal target in designing novel analgesics. This review will focus on the novel insights into the mGluR8 role on pain control, with particular emphasis on the supraspinal descending pathway, an antinociceptive endogenous source, whose activation or disinhibition (via mGluR8) induces analgesia.

Series-counterpulse repetitive-pulse inductive storage circuit

DOEpatents

Honig, E.M.

1984-06-05

A high-power series-counterpulse repetitive-pulse inductive energy storage and transfer circuit includes an opening switch, a main energy storage coil, and a counterpulse capacitor. The local pulse is initiated simultaneously with the initiation of the counterpulse used to turn the opening switch off. There is no delay from command to output pulse. During the load pulse, the counterpulse capacitor is automatically charged with sufficient energy to accomplish the load counterpulse which terminates the load pulse and turns the load switch off. When the main opening switch is reclosed to terminate the load pulse, the counterpulse capacitor discharges through the load, causing a rapid, sharp cutoff of the load pulse as well as recovering any energy remaining in the load inductance. The counterpulse capacitor is recharged to its original condition by the main energy storage coil after the load pulse is over, not before it begins.

COS/FUV Special Recovery

NASA Astrophysics Data System (ADS)

Wheeler, Thomas

2011-10-01

This proposal consists of the steps necessary for turning on and ramping up {in magnitude} the COS FUV high voltage and returning the FUV detector to science operations in a conservative manner after a HV anomalous or burst event {similar to what has been seen on FUSE} shutdown. HSTAR 13305 provides details and timeline of the present shutdown. The complete step-by-step procedure is detailed in the Observing Description, but in summary, the following is done: Groups of sequences with one dayâ??s activities with the QE grid off and one dayâ??s activities with the QE grid on alternate to progressively higher voltages with the QE off group leading by one sequence. The initial HV turn on {Day 1} occurs with the QE grid off; the initial transition from FUV Operate to HVLow is broken into two parts with a 1- hour wait between turning the HV on and ramping to HVLow {SAA}. The next Day 1 visit is 1 hour later, where the HV is turned off by returning the FUV Operate state. After another1-hour wait, the QE grid is turned on, the HV is turned on and the voltage is not ramped. After another 1-hour wait, the HV is turned off returning to FUV Operate. The Day 1 ends with the NSSC-1 COS flag 3 is set to prevent any FUV HV commanding. This is followed by 10 cycles {2 * 5 cycles - one set of cycles with the grid off and one set with the grid on} of HV ramp-ups and returns to FUV Operate {HV off} alternating between grid off and on with the QE off group leading by one HV setting. The HV Commanded Counts for each group of cycles are: 154/151, 160/157, 166/163, 172/169, HVNom {178/175}. {The formula to convert Command Counts to HV is: Volts = {Command Counts * -15.69V} -2500V.}The QE off and QE on cycles are similar except for the QE commanding. One typical QE off cycle is shown below:V14 QE off - Ramp to 166/163After V10 by 2D for analysis. Flag 3 must be cleared to execute. 1. QE off - Turn HV on 2. Ramp to HVLow {100/100} 3. Ramp HV to 166/163 4. DCE RAM dump 5. Dark exposure 6. Wave exposureV15 Return to OperateAfter V14 by 1hr 1. Dark exposure 2. Wave exposure 3. Return to HVLow {100/100} 4. Return to Operate {HV off} 5. DCE RAM Dump 6. Set flag 3In some later cycles with the QE grid on, ground system QasiStates are used to auto-schedule the Operate to HVNom transition. Visits 21 and 25 are going to HVNom {178/175} with the QE grid off and on, respectively. There will be a gap of 2days between grid-off cycles and 2 days between grid-on cycles, offset by 1 day. {See the proposal description for exact timing.}All HV ramp-up will be done at the nominal value of 3 seconds per HV "step" rather than 10 seconds per HV "step" used in SMOV. The concern during SMOV was that gas exposure during launch would allow gas to adsorb on the MCP pore surfaces, and that slower ramping would help to remove this excess gas. This concern no longer exists. The cycle voltage values {for Segments A and B} must be patched in FSW in each cycle prior to the HV ramp commanding. Memory monitors will be set on the patched memory locations. Immediately after obtaining the commanded voltage for that cycle and after return to FUV Operate {HV-off} commanding, the DCE memory will be dumped. After HV ramp-up commanding starting with HVLow and prior to returning to HV off, short DARK exposures {300 secs.} with Stim Rate = 2000 will be obtained and after HV ramp-up commanding starting with levels above HVLow and prior to returning to HV off, short WAVE exposures {60 secs.} will be obtained. After all visits that end with Return to Operate {HV off}, NSSC-1 COS event flag 3 will be set to inhibit any FUV commanding, a.k.a. a â??dead manâ??sâ?? switch. If the flag remains set, subsequent FUV commanding will be skipped. Thus, Operations Requests must be in place to clear the flag prior to those subsequent visits. Real-time monitoring of the telemetry will be used to guide the decisions whether or not to clear the flag. This is also required after the final visit.Throughout the proposal, different â??after byâ?? times, sequence containers, and new alignments are used to optimize flow, schedulability, telemetry and science data analyses, and the clearing of flag 3. The proposal is designed such that the visits and exposures MUST be executed in order.Additionally, all visits are compliant with CARD 3.4.12.8 - COS FUV Mandatory Dwell Time at HVLow {1 hour dwell at HVLow before ramping to a more negative voltage} and CARD 3.4.12.9 â?? COS FUV High Voltage QE Grid Operation {HV must be less negative or equal to the HVLow to switch grid on or off}.

The Gaia-ESO Survey: evidence of atomic diffusion in M67?

NASA Astrophysics Data System (ADS)

Bertelli Motta, C.; Pasquali, A.; Richer, J.; Michaud, G.; Salaris, M.; Bragaglia, A.; Magrini, L.; Randich, S.; Grebel, E. K.; Adibekyan, V.; Blanco-Cuaresma, S.; Drazdauskas, A.; Fu, X.; Martell, S.; Tautvaišienė, G.; Gilmore, G.; Alfaro, E. J.; Bensby, T.; Flaccomio, E.; Koposov, S. E.; Korn, A. J.; Lanzafame, A. C.; Smiljanic, R.; Bayo, A.; Carraro, G.; Casey, A. R.; Costado, M. T.; Damiani, F.; Franciosini, E.; Heiter, U.; Hourihane, A.; Jofré, P.; Lardo, C.; Lewis, J.; Monaco, L.; Morbidelli, L.; Sacco, G. G.; Sousa, S. G.; Worley, C. C.; Zaggia, S.

2018-07-01

Investigating the chemical homogeneity of stars born from the same molecular cloud at virtually the same time is very important for our understanding of the chemical enrichment of the interstellar medium and with it the chemical evolution of the Galaxy. One major cause of inhomogeneities in the abundances of open clusters is stellar evolution of the cluster members. In this work, we investigate variations in the surface chemical composition of member stars of the old open cluster M67 as a possible consequence of atomic diffusion effects taking place during the main-sequence phase. The abundances used are obtained from high-resolution UVES/FLAMES spectra within the framework of the Gaia-ESO Survey. We find that the surface abundances of stars on the main sequence decrease with increasing mass reaching a minimum at the turn-off. After deepening of the convective envelope in subgiant branch stars, the initial surface abundances are restored. We found the measured abundances to be consistent with the predictions of stellar evolutionary models for a cluster with the age and metallicity of M67. Our findings indicate that atomic diffusion poses a non-negligible constraint on the achievable precision of chemical tagging methods.

The Gaia-ESO Survey: Evidence of atomic diffusion in M67?

NASA Astrophysics Data System (ADS)

Motta, C. Bertelli; Pasquali, A.; Richer, J.; Michaud, G.; Salaris, M.; Bragaglia, A.; Magrini, L.; Randich, S.; Grebel, E. K.; Adibekyan, V.; Blanco-Cuaresma, S.; Drazdauskas, A.; Fu, X.; Martell, S.; TautvaišienÄ--, G.; Gilmore, G.; Alfaro, E. J.; Bensby, T.; Flaccomio, E.; Koposov, S. E.; Korn, A. J.; Lanzafame, A. C.; Smiljanic, R.; Bayo, A.; Carraro, G.; Casey, A. R.; Costado, M. T.; Damiani, F.; Franciosini, E.; Heiter, U.; Hourihane, A.; Jofré, P.; Lardo, C.; Lewis, J.; Monaco, L.; Morbidelli, L.; Sacco, G. G.; Sousa, S. G.; Worley, C. C.; Zaggia, S.

2018-04-01

Investigating the chemical homogeneity of stars born from the same molecular cloud at virtually the same time is very important for our understanding of the chemical enrichment of the interstellar medium and with it the chemical evolution of the Galaxy. One major cause of inhomogeneities in the abundances of open clusters is stellar evolution of the cluster members. In this work, we investigate variations in the surface chemical composition of member stars of the old open cluster M67 as a possible consequence of atomic diffusion effects taking place during the main-sequence phase. The abundances used are obtained from high-resolution UVES/FLAMES spectra within the framework of the Gaia-ESO Survey. We find that the surface abundances of stars on the main sequence decrease with increasing mass reaching a minimum at the turn-off. After deepening of the convective envelope in sub-giant branch stars, the initial surface abundances are restored. We found the measured abundances to be consistent with the predictions of stellar evolutionary models for a cluster with the age and metallicity of M67. Our findings indicate that atomic diffusion poses a non-negligible constraint on the achievable precision of chemical tagging methods.

Free energy determinants of secondary structure formation: III. beta-turns and their role in protein folding.

PubMed

Yang, A S; Hitz, B; Honig, B

1996-06-21

The stability of beta-turns is calculated as a function of sequence and turn type with a Monte Carlo sampling technique. The conformational energy of four internal hydrogen-bonded turn types, I, I', II and II', is obtained by evaluating their gas phase energy with the CHARMM force field and accounting for solvation effects with the Finite Difference Poisson-Boltzmann (FDPB) method. All four turn types are found to be less stable than the coil state, independent of the sequence in the turn. The free-energy penalties associated with turn formation vary between 1.6 kcal/mol and 7.7 kcal/mol, depending on the sequence and turn type. Differences in turn stability arise mainly from intraresidue interactions within the two central residues of the turn. For each combination of the two central residues, except for -Gly-Gly-, the most stable beta-turn type is always found to occur most commonly in native proteins. The fact that a model based on local interactions accounts for the observed preference of specific sequences suggests that long-range tertiary interactions tend to play a secondary role in determining turn conformation. In contrast, for beta-hairpins, long-range interactions appear to dominate. Specifically, due to the right-handed twist of beta-strands, type I' turns for -Gly-Gly- are found to occur with high frequency, even when local energetics would dictate otherwise. The fact that any combination of two residues is found able to adopt a relatively low-energy turn structure explains why the amino acid sequence in turns is highly variable. The calculated free-energy cost of turn formation, when combined with related numbers obtained for alpha-helices and beta-sheets, suggests a model for the initiation of protein folding based on metastable fragments of secondary structure.

Circumstellar Material on and off the Main Sequence

NASA Astrophysics Data System (ADS)

Steele, Amy; Debes, John H.; Deming, Drake

2017-06-01

There is evidence of circumstellar material around main sequence, giant, and white dwarf stars that originates from the small-body population of planetary systems. These bodies tell us something about the chemistry and evolution of protoplanetary disks and the planetary systems they form. What happens to this material as its host star evolves off the main sequence, and how does that inform our understanding of the typical chemistry of rocky bodies in planetary systems? In this talk, I will discuss the composition(s) of circumstellar material on and off the main sequence to begin to answer the question, “Is Earth normal?” In particular, I look at three types of debris disks to understand the typical chemistry of planetary systems—young debris disks, debris disks around giant stars, and dust around white dwarfs. I will review the current understanding on how to infer dust composition for each class of disk, and present new work on constraining dust composition from infrared excesses around main sequence and giant stars. Finally, dusty and polluted white dwarfs hold a unique key to our understanding of the composition of rocky bodies around other stars. In particular, I will discuss WD1145+017, which has a transiting, disintegrating planetesimal. I will review what we know about this system through high speed photometry and spectroscopy and present new work on understanding the complex interplay of physics that creates white dwarf pollution from the disintegration of rocky bodies.

Symmetry energies for A =24 and 48 and the USD and KB3 shell model Hamiltonians

NASA Astrophysics Data System (ADS)

Kingan, A.; Neergârd, K.; Zamick, L.

2017-12-01

Calculations in the sd and pf shells reported some time ago by Satuła et al. [Phys. Lett. B 407, 103 (1997), 10.1016/S0370-2693(97)00711-9] are redone for an extended analysis of the results. As in the original work, we do calculations for one mass number in each shell and consider in each case the sequence of lowest energies for isospins 0, 2, and 4, briefly the symmetry spectrum. Following further the original work, we study how this spectrum changes when parts of the two-nucleon interaction are turned off. The variation of its width is explored in detail. A differential combination ɛW of the three energies was taken in the original work as a measure of the so-called Wigner term in semiempirical mass formulas, and it was found to decrease drastically when the two-nucleon interaction in the channel of zero isospin is turned off. Our analysis shows that the width of the symmetry spectrum experiences an equally drastic decrease, which can be explained qualitatively in terms of schematic approximations. We therefore suggest that the decrease of ɛW be seen mainly as a side effect of a narrowing of the symmetry spectrum rather than an independent manifestation of the two-nucleon interaction in the channel of zero isospin.

Efficiency of static core turn-off in a system-on-a-chip with variation

DOEpatents

Cher, Chen-Yong; Coteus, Paul W; Gara, Alan; Kursun, Eren; Paulsen, David P; Schuelke, Brian A; Sheets, II, John E; Tian, Shurong

2013-10-29

A processor-implemented method for improving efficiency of a static core turn-off in a multi-core processor with variation, the method comprising: conducting via a simulation a turn-off analysis of the multi-core processor at the multi-core processor's design stage, wherein the turn-off analysis of the multi-core processor at the multi-core processor's design stage includes a first output corresponding to a first multi-core processor core to turn off; conducting a turn-off analysis of the multi-core processor at the multi-core processor's testing stage, wherein the turn-off analysis of the multi-core processor at the multi-core processor's testing stage includes a second output corresponding to a second multi-core processor core to turn off; comparing the first output and the second output to determine if the first output is referring to the same core to turn off as the second output; outputting a third output corresponding to the first multi-core processor core if the first output and the second output are both referring to the same core to turn off.

Differences between main-channel and off-channel food webs in the upper Mississippi River revealed by fatty acid profiles of consumers

USGS Publications Warehouse

Larson, James H.; Bartsch, Michelle; Gutreuter, Steve; Knights, Brent C.; Bartsch, Lynn; Richardson, William B.; Vallazza, Jonathan M.; Arts, Michael T.

2015-01-01

Large river systems are often thought to contain a mosaic of patches with different habitat characteristics driven by differences in flow and mixing environments. Off-channel habitats (e.g., backwater areas, secondary channels) can become semi-isolated from main-channel water inputs, leading to the development of distinct biogeochemical environments. Observations of adult bluegill (Lepomis macrochirus) in the main channel of the Mississippi River led to speculation that the main channel offered superior food resources relative to off-channel areas. One important aspect of food quality is the quantity and composition of polyunsaturated fatty acids (PUFA). We sampled consumers from main-channel and backwater habitats to determine whether they differed in PUFA content. Main-channel individuals for relatively immobile species (young-of-year bluegill, zebra mussels [Dreissena polymorpha], and plain pocketbook mussels [Lampsilis cardium]) had significantly greater PUFA content than off-channel individuals. No difference in PUFA was observed for the more mobile gizzard shad (Dorsoma cepedianum), which may move between main-channel and off-channel habitats even at early life-history stages. As off-channel habitats become isolated from main-channel waters, flow and water column nitrogen decrease, potentially improving conditions for nitrogen-fixing cyanobacteria and vascular plants that, in turn, have low PUFA content. We conclude that main-channel food webs of the upper Mississippi River provide higher quality food resources for some riverine consumers as compared to food webs in off-channel habitats.

Exploring halo substructure with giant stars. XIV. The nature of the Triangulum-Andromeda stellar features

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sheffield, Allyson A.; Johnston, Kathryn V.; Majewski, Steven R.

As large-scale stellar surveys have become available over the past decade, the ability to detect and characterize substructures in the Galaxy has increased dramatically. These surveys have revealed the Triangulum-Andromeda (TriAnd) region to be rich with substructures in the distance range 20-30 kpc, and the relation of these features to each other, if any, remains unclear. An exploration using Two Micron All Sky Survey (2MASS) photometry reveals not only the faint sequence in M giants detected by Rocha-Pinto et al. spanning the range 100° < l < 160° and –50° < b < –15°, but, in addition, a second, brightermore » and more densely populated sequence. These sequences are likely associated with the distinct main sequences (MSs) discovered (and labeled TriAnd1 and TriAnd2) by Martin et al. in an optical survey in the direction of M31, where TriAnd2 is the optical counterpart of the fainter red giant branch (RGB)/asymptotic giant branch sequence of Rocha-Pinto et al. Here, the age, distance, and metallicity ranges for TriAnd1 and TriAnd2 are estimated by simultaneously fitting isochrones to the 2MASS RGB tracks and the optical MS/MS turn-off features. The two populations are clearly distinct in age and distance: the brighter sequence (TriAnd1) is younger (6-10 Gyr) and closer (distance of ∼15-21 kpc), whereas the fainter sequence (TriAnd2) is older (10-12 Gyr) and at an estimated distance of ∼24-32 kpc. A comparison with simulations demonstrates that the differences and similarities between TriAnd1 and TriAnd2 can simultaneously be explained if they represent debris originating from the disruption of the same dwarf galaxy, but torn off during two distinct pericentric passages.« less

Predicting turns in proteins with a unified model.

PubMed

Song, Qi; Li, Tonghua; Cong, Peisheng; Sun, Jiangming; Li, Dapeng; Tang, Shengnan

2012-01-01

Turns are a critical element of the structure of a protein; turns play a crucial role in loops, folds, and interactions. Current prediction methods are well developed for the prediction of individual turn types, including α-turn, β-turn, and γ-turn, etc. However, for further protein structure and function prediction it is necessary to develop a uniform model that can accurately predict all types of turns simultaneously. In this study, we present a novel approach, TurnP, which offers the ability to investigate all the turns in a protein based on a unified model. The main characteristics of TurnP are: (i) using newly exploited features of structural evolution information (secondary structure and shape string of protein) based on structure homologies, (ii) considering all types of turns in a unified model, and (iii) practical capability of accurate prediction of all turns simultaneously for a query. TurnP utilizes predicted secondary structures and predicted shape strings, both of which have greater accuracy, based on innovative technologies which were both developed by our group. Then, sequence and structural evolution features, which are profile of sequence, profile of secondary structures and profile of shape strings are generated by sequence and structure alignment. When TurnP was validated on a non-redundant dataset (4,107 entries) by five-fold cross-validation, we achieved an accuracy of 88.8% and a sensitivity of 71.8%, which exceeded the most state-of-the-art predictors of certain type of turn. Newly determined sequences, the EVA and CASP9 datasets were used as independent tests and the results we achieved were outstanding for turn predictions and confirmed the good performance of TurnP for practical applications.

Predicting Turns in Proteins with a Unified Model

PubMed Central

Song, Qi; Li, Tonghua; Cong, Peisheng; Sun, Jiangming; Li, Dapeng; Tang, Shengnan

2012-01-01

Motivation Turns are a critical element of the structure of a protein; turns play a crucial role in loops, folds, and interactions. Current prediction methods are well developed for the prediction of individual turn types, including α-turn, β-turn, and γ-turn, etc. However, for further protein structure and function prediction it is necessary to develop a uniform model that can accurately predict all types of turns simultaneously. Results In this study, we present a novel approach, TurnP, which offers the ability to investigate all the turns in a protein based on a unified model. The main characteristics of TurnP are: (i) using newly exploited features of structural evolution information (secondary structure and shape string of protein) based on structure homologies, (ii) considering all types of turns in a unified model, and (iii) practical capability of accurate prediction of all turns simultaneously for a query. TurnP utilizes predicted secondary structures and predicted shape strings, both of which have greater accuracy, based on innovative technologies which were both developed by our group. Then, sequence and structural evolution features, which are profile of sequence, profile of secondary structures and profile of shape strings are generated by sequence and structure alignment. When TurnP was validated on a non-redundant dataset (4,107 entries) by five-fold cross-validation, we achieved an accuracy of 88.8% and a sensitivity of 71.8%, which exceeded the most state-of-the-art predictors of certain type of turn. Newly determined sequences, the EVA and CASP9 datasets were used as independent tests and the results we achieved were outstanding for turn predictions and confirmed the good performance of TurnP for practical applications. PMID:23144872

Morphometry of the coronary ostia and the structure of coronary arteries in the shorthair domestic cat

PubMed Central

Barszcz, Karolina; Kupczyńska, Marta; Klećkowska-Nawrot, Joanna; Janeczek, Maciej; Goździewska-Harłajczuk, Karolina; Dzierzęcka, Małgorzata; Janczyk, Paweł

2017-01-01

The aim of this study was to measure the area of the coronary ostia, assess their localization in the coronary sinuses and to determine the morphology of the stem of the left and right coronary arteries in the domestic shorthair cat. The study was conducted on 100 hearts of domestic shorthair cats of both sexes, aged 2–18 years, with an average body weight of 4.05 kg. A morphometric analysis of the coronary ostia was carried out on 52 hearts. The remaining 48 hearts were injected with a casting material in order to carry out a morphological assessment of the left and right coronary arteries. In all the studied animals, the surface of the left coronary artery ostium was larger than the surface of the right coronary artery ostium. There were four types of the left main coronary artery: type I (23 animals, 49%)–double-branched left main stem (giving off the left circumflex branch and the interventricular paraconal branch, which in turn gave off the septal branch), type II (12 animals, 26%)–double-branched left main stem (giving off the left circumflex branch and the interventricular paraconal branch without the septal branch), type III (11 animals, 23%)–triple-branched left main stem (giving off the left circumflex branch, interventricular branch and the septal branch, type IV (1 animal, 2%)–double-branched left main stem (giving off the interventricular paraconal branch and the left circumflex branch, which in turn gave off the septal branch). The left coronary artery ostium is greater than the right one. There is considerable diversity in the branches of proximal segment of the left coronary artery, while the right coronary artery is more conservative. These results can be useful in defining the optimal strategies in the endovascular procedures involving the coronary arteries or the aortic valve in the domestic shorthair cat. PMID:29020103

Power processor for a 20CM ion thruster

NASA Technical Reports Server (NTRS)

Biess, J. J.; Schoenfeld, A. D.; Cohen, E.

1973-01-01

A power processor breadboard for the JPL 20CM Ion Engine was designed, fabricated, and tested to determine compliance with the electrical specification. The power processor breadboard used the silicon-controlled rectifier (SCR) series resonant inverter as the basic power stage to process all the power to the ion engine. The breadboard power processor was integrated with the JPL 20CM ion engine and complete testing was performed. The integration tests were performed without any silicon-controlled rectifier failure. This demonstrated the ruggedness of the series resonant inverter in protecting the switching elements during arcing in the ion engine. A method of fault clearing the ion engine and returning back to normal operation without elaborate sequencing and timing control logic was evolved. In this method, the main vaporizer was turned off and the discharge current limit was reduced when an overload existed on the screen/accelerator supply. After the high voltage returned to normal, both the main vaporizer and the discharge were returned to normal.

Series-counterpulse repetitive-pulse inductive storage circuit

DOEpatents

Honig, Emanuel M.

1986-01-01

A high-power series-counterpulse repetitive-pulse inductive energy storage and transfer circuit includes an opening switch, a main energy storage coil, and a counterpulse capacitor. The load pulse is initiated simultaneously with the initiation of the counterpulse which is used to turn the opening switch off. There is no delay from command to output pulse. During the load pulse, the counterpulse capacitor is first discharged and then recharged in the opposite polarity with sufficient energy to accomplish the load counterpulse which terminates the load pulse and turns the load switch off. When the main opening switch is triggered closed again to terminate the load pulse, the counterpulse capacitor discharges in the reverse direction through the load switch and through the load, causing a rapid, sharp cutoff of the load pulse as well as recovering any energy remaining in the load inductance. The counterpulse capacitor is recharged to its original condition by the main energy storage coil after the load pulse is over, not before it begins.

A new inverter topology using GTO commutation. [Gate Turn Off thyristor

NASA Technical Reports Server (NTRS)

Rippel, W. E.

1983-01-01

A new N-phase, forced commutated bridge inverter topology has been developed wherein a single Gate Turn Off Thyristor (GTO) is used to commutate each of 2N main Thyristors (SCRs). Since, for most applications, the primary loss mechanism is the SCR forward drop, very high efficiencies are possible. Compared with conventional pure SCR and pure GTO inverters, cost per kW is lower - in the former case due to the large cost differential between GTOs and SCRs. Other advantages of the new inverter include high power density, low switching losses and stresses, modulation flexibility and amenability to high voltage and high frequency operation.

ChIP-Seq Analysis for Identifying Genome-Wide Histone Modifications Associated with Stress-Responsive Genes in Plants.

PubMed

Li, Guosheng; Jagadeeswaran, Guru; Mort, Andrew; Sunkar, Ramanjulu

2017-01-01

Histone modifications represent the crux of epigenetic gene regulation essential for most biological processes including abiotic stress responses in plants. Thus, identification of histone modifications at the genome-scale can provide clues for how some genes are 'turned-on' while some others are "turned-off" in response to stress. This chapter details a step-by-step protocol for identifying genome-wide histone modifications associated with stress-responsive gene regulation using chromatin immunoprecipitation (ChIP) followed by sequencing of the DNA (ChIP-seq).

iss042e136099

NASA Image and Video Library

2015-01-15

ISS042e136099 (Jan 15, 20105) -- Interior view of the Columbus European Laboratory taken during the crew's sleep period (main lights are turned off). The pink glow comes from the Vegetable Production System (Veggie) greenhouse, housed in the module.

SAGITTARIUS STREAM THREE-DIMENSIONAL KINEMATICS FROM SLOAN DIGITAL SKY SURVEY STRIPE 82

DOE Office of Scientific and Technical Information (OSTI.GOV)

Koposov, Sergey E.; Belokurov, Vasily; Evans, N. Wyn

2013-04-01

Using multi-epoch observations of the Stripe 82 region from the Sloan Digital Sky Survey (SDSS), we measure precise statistical proper motions of the stars in the Sagittarius (Sgr) stellar stream. The multi-band photometry and SDSS radial velocities allow us to efficiently select Sgr members and thus enhance the proper-motion precision to {approx}0.1 mas yr{sup -1}. We measure separately the proper motion of a photometrically selected sample of the main-sequence turn-off stars, as well as spectroscopically selected Sgr giants. The data allow us to determine the proper motion separately for the two Sgr streams in the south found in Koposov etmore » al. Together with the precise velocities from SDSS, our proper motions provide exquisite constraints of the three-dimensional motions of the stars in the Sgr streams.« less

The STREGA survey - II. Globular cluster Palomar 12

NASA Astrophysics Data System (ADS)

Musella, I.; Di Criscienzo, M.; Marconi, M.; Raimondo, G.; Ripepi, V.; Cignoni, M.; Bono, G.; Brocato, E.; Dall'Ora, M.; Ferraro, I.; Grado, A.; Iannicola, G.; Limatola, L.; Molinaro, R.; Moretti, M. I.; Stetson, P. B.; Capaccioli, M.; Cioni, M.-R. L.; Getman, F.; Schipani, P.

2018-01-01

In the framework of the STREGA (STRucture and Evolution of the GAlaxy) survey, two fields around the globular cluster Pal 12 were observed with the aim of detecting the possible presence of streams and/or an extended halo. The adopted stellar tracers are the main sequence, turn-off and red giant branch stars. We discuss the luminosity function and the star counts in the observed region covering about 2 tidal radii, confirming that Pal 12 appears to be embedded in the Sagittarius Stream. Adopting an original approach to separate cluster and field stars, we do not find any evidence of significant extra-tidal Pal 12 stellar populations. The presence of the Sagittarius stream seems to have mimicked a larger tidal radius in previous studies. Indeed, adopting a King model, a redetermination of this value gives rT = 0.22 ± 0.1 deg.

Exploring the nature and synchronicity of early cluster formation in the Large Magellanic Cloud - II. Relative ages and distances for six ancient globular clusters

NASA Astrophysics Data System (ADS)

Wagner-Kaiser, R.; Mackey, Dougal; Sarajedini, Ata; Chaboyer, Brian; Cohen, Roger E.; Yang, Soung-Chul; Cummings, Jeffrey D.; Geisler, Doug; Grocholski, Aaron J.

2017-11-01

We analyse Hubble Space Telescope observations of six globular clusters in the Large Magellanic Cloud (LMC) from programme GO-14164 in Cycle 23. These are the deepest available observations of the LMC globular cluster population; their uniformity facilitates a precise comparison with globular clusters in the Milky Way. Measuring the magnitude of the main-sequence turn-off point relative to template Galactic globular clusters allows the relative ages of the clusters to be determined with a mean precision of 8.4 per cent, and down to 6 per cent for individual objects. We find that the mean age of our LMC cluster ensemble is identical to the mean age of the oldest metal-poor clusters in the Milky Way halo to 0.2 ± 0.4 Gyr. This provides the most sensitive test to date of the synchronicity of the earliest epoch of globular cluster formation in two independent galaxies. Horizontal branch magnitudes and subdwarf fitting to the main sequence allow us to determine distance estimates for each cluster and examine their geometric distribution in the LMC. Using two different methods, we find an average distance to the LMC of 18.52 ± 0.05.

Application of State Quantization-Based Methods in HEP Particle Transport Simulation

NASA Astrophysics Data System (ADS)

Santi, Lucio; Ponieman, Nicolás; Jun, Soon Yung; Genser, Krzysztof; Elvira, Daniel; Castro, Rodrigo

2017-10-01

Simulation of particle-matter interactions in complex geometries is one of the main tasks in high energy physics (HEP) research. An essential aspect of it is an accurate and efficient particle transportation in a non-uniform magnetic field, which includes the handling of volume crossings within a predefined 3D geometry. Quantized State Systems (QSS) is a family of numerical methods that provides attractive features for particle transportation processes, such as dense output (sequences of polynomial segments changing only according to accuracy-driven discrete events) and lightweight detection and handling of volume crossings (based on simple root-finding of polynomial functions). In this work we present a proof-of-concept performance comparison between a QSS-based standalone numerical solver and an application based on the Geant4 simulation toolkit, with its default Runge-Kutta based adaptive step method. In a case study with a charged particle circulating in a vacuum (with interactions with matter turned off), in a uniform magnetic field, and crossing up to 200 volume boundaries twice per turn, simulation results showed speedups of up to 6 times in favor of QSS while it being 10 times slower in the case with zero volume boundaries.

iss042e136094

NASA Image and Video Library

2015-01-15

ISS042e136094 (Jan 15, 2015) -- Interior view looking forward (FWD) in the Destiny U.S. Laboratory during the crew's sleep period, with the main lights turned off. The pink glow comes from the Vegetable Production System (Veggie) greenhouse, housed in the Columbus European Laboratory.

A detached stellar-mass black hole candidate in the globular cluster NGC 3201

NASA Astrophysics Data System (ADS)

Giesers, Benjamin; Dreizler, Stefan; Husser, Tim-Oliver; Kamann, Sebastian; Anglada Escudé, Guillem; Brinchmann, Jarle; Carollo, C. Marcella; Roth, Martin M.; Weilbacher, Peter M.; Wisotzki, Lutz

2018-03-01

As part of our massive spectroscopic survey of 25 Galactic globular clusters with MUSE, we performed multiple epoch observations of NGC 3201 with the aim of constraining the binary fraction. In this cluster, we found one curious star at the main-sequence turn-off with radial velocity variations of the order of 100 km s- 1, indicating the membership to a binary system with an unseen component since no other variations appear in the spectra. Using an adapted variant of the generalized Lomb-Scargle periodogram, we could calculate the orbital parameters and found the companion to be a detached stellar-mass black hole with a minimum mass of 4.36 ± 0.41 M⊙. The result is an important constraint for binary and black hole evolution models in globular clusters as well as in the context of gravitational wave sources.

Hairpin stabilized fluorescent silver nanoclusters for quantitative detection of NAD+ and monitoring NAD+/NADH based enzymatic reactions.

PubMed

Jain, Priyamvada; Chakma, Babina; Patra, Sanjukta; Goswami, Pranab

2017-03-01

A set of 90 mer long ssDNA candidates, with different degrees of cytosine (C-levels) (% and clusters) was analyzed for their function as suitable Ag-nanocluster (AgNC) nucleation scaffolds. The sequence (P4) with highest C-level (42.2%) emerged as the only candidate supporting the nucleation process as evident from its intense fluorescence peak at λ 660 nm . Shorter DNA subsets derived from P4 with only stable hairpin structures could support the AgNC formation. The secondary hairpin structures were confirmed by PAGE, and CD studies. The number of base pairs in the stem region also contributes to the stability of the hairpins. A shorter 29 mer sequence (Sub 3) (ΔG = -1.3 kcal/mol) with 3-bp in the stem of a 7-mer loop conferred highly stable AgNC. NAD + strongly quenched the fluorescence of Sub 3-AgNC in a concentration dependent manner. Time resolved photoluminescence studies revealed the quenching involves a combined static and dynamic interaction where the binding constant and number of binding sites for NAD + were 0.201 L mol -1 and 3.6, respectively. A dynamic NAD + detection range of 50-500 μM with a limit of detection of 22.3 μM was discerned. The NAD + mediated quenching of AgNC was not interfered by NADH, NADP + , monovalent and divalent ions, or serum samples. The method was also used to follow alcohol dehydrogenase and lactate dehydrogenase catalyzed physiological reactions in a turn-on and turn-off assay, respectively. The proposed method with ssDNA-AgNC could therefore be extended to monitor other NAD + /NADH based enzyme catalyzed reactions in a turn-on/turn-off approach. Copyright © 2016 Elsevier B.V. All rights reserved.

High voltage and current, gate assisted, turn-off thyristor development

NASA Technical Reports Server (NTRS)

Nowalk, T. P.; Brewster, J. B.; Kao, Y. C.

1972-01-01

An improved high speed power switch with unique turn-off capability was developed. This gate assisted turn-off thyristor (GATT) was rated 1000 volts and 100 amperes with turn-off times of 2 microseconds. Fifty units were delivered for evaluation. In addition, test circuits designed to relate to the series inverter application were built and demonstrated. In the course of this work it was determined that the basic device design is adequate to meet the static characteristics and dynamic turn-off specification. It was further determined that the turn-on specification is critically dependent on the gate drive circuit due to the distributive nature of the cathode-gate geometry. Future work should emphasize design modifications which reduce the gate current required for fast turn-on, thereby opening the way to higher power (current) devices.

Protective Socket For Integrated Circuits

NASA Technical Reports Server (NTRS)

Wilkinson, Chris; Henegar, Greg

1988-01-01

Socket for intergrated circuits (IC's) protects from excessive voltages and currents or from application of voltages and currents in wrong sequence during insertion or removal. Contains built-in switch that opens as IC removed, disconnecting leads from signals and power. Also protects other components on circuit board from transients produced by insertion and removal of IC. Makes unnecessary to turn off power to entire circuit board so other circuits on board continue to function.

Interior View in Node 2

NASA Image and Video Library

2015-01-15

Interior view looking starboard (STBD) and aft in the Harmony Node 2, taken during the crew's sleep period (main lights are turned off). Hatches into the Columbus European Laboratory and Destiny U.S. Laboratory are in view. The pink glow comes from the Vegetable Production System (Veggie) greenhouse, housed in Columbus.

Stellar Populations with the LSST

NASA Astrophysics Data System (ADS)

Saha, Abhijit; Olsen, K.; LSST Stellar Populations Collaboration

2006-12-01

The LSST will produce a multi-color map and photometric object catalog of half the sky to g 27.5(5σ). Strategically cadenced time-space sampling of each field spanning ten years will allow variability, proper motion and parallax measurements for objects brighter than g 25. As part of providing an unprecedented map of the Galaxy, the accurate multi-band photometry will permit photometric parallaxes, chemical abundances and a handle on ages via colors at turn-off for main-sequence stars at all distances within the Galaxy, permitting a comprehensive study of star formation histories (SFH) and chemical evolution for field stars. With a geometric parallax accuracy of 1mas, LSST will produce a robust complete sample of the solar neighborhood stars. While delivering parallax accuracy comparable to HIPPARCOS, LSST will extend the catalog to more than a 10 magnitudes fainter limit, and will be complete to MV 15. In the Magellanic Clouds too, the photometry will reach MV +8, allowing the SFH and chemical signatures in the expansive outer extremities to be gleaned from their main sequence stars. This in turn will trace the detailed interaction of the Clouds with the Galaxy halo. The LSST time sampling will identify and characterize variable stars of all types, from time scales of 1hr to several years, a feast for variable star astrophysics. Cepheids and LPVs in all galaxies in the Sculptor, M83 and Cen-A groups are obvious data products: comparative studies will reveal systematic differences with galaxy properties, and help to fine tune the rungs of the distance ladder. Dwarf galaxies within 10Mpc that are too faint to find from surface brightness enhancements will be revealed via over-densities of their red giants: this systematic census will extend the luminosity function of galaxies to the faint limit. Novae discovered by LSST time sampling will trace intergalactic stars out to the Virgo and Fornax clusters.

Development of a 1000V, 200A, low-loss, fast-switching, gate-assisted turn-off thyristor

NASA Technical Reports Server (NTRS)

Schlegel, E. S.; Lowry, L. R.

1975-01-01

Feasibility was demonstrated for a thyristor that blocks 1000V forward and reverse, conducts 200A, and turns on in little more than 2 microsec with only 2A of gate drive. Its features include a turn-off time of 3 microsec achieved with 2A of gate assist current of a few microseconds duration and an energy dissipation of only 12 mJ per pulse for a 20 microsec half sine wave, 200A pulse. Extensive theoretical and experimental study of the electrical behavior of thyristors having a fast turn-off time have significantly improved the understanding of the physics of turning thyristor off. Thyristors of two new designs were fabricated and evaluated. The high speed and low power were achieved by a combination of gate amplification, cathode shunting, and gate-assisted turn-off. Two techniques for making this combination practical are described.

Unexplored Potentials of Epigenetic Mechanisms of Plants and Animals—Theoretical Considerations

PubMed Central

Seffer, Istvan; Nemeth, Zoltan; Hoffmann, Gyula; Matics, Robert; Seffer, A Gergely; Koller, Akos

2013-01-01

Morphological and functional changes of cells are important for adapting to environmental changes and associated with continuous regulation of gene expressions. Genes are regulated–in part–by epigenetic mechanisms resulting in alternating patterns of gene expressions throughout life. Epigenetic changes responding to the environmental and intercellular signals can turn on/off specific genes, but do not modify the DNA sequence. Most epigenetic mechanisms are evolutionary conserved in eukaryotic organisms, and several homologs of epigenetic factors are present in plants and animals. Moreover, in vitro studies suggest that the plant cytoplasm is able to induce a nuclear reassembly of the animal cell, whereas others suggest that the ooplasm is able to induce condensation of plant chromatin. Here, we provide an overview of the main epigenetic mechanisms regulating gene expression and discuss fundamental epigenetic mechanisms and factors functioning in both plants and animals. Finally, we hypothesize that animal genome can be reprogrammed by epigenetic factors from the plant protoplast. PMID:25512705

The DECam Plane Survey: Optical photometry of two billion objects in the southern Galactic plane

NASA Astrophysics Data System (ADS)

Schlafly, Edward; Green, Gregory M.; Lang, Dustin; Daylan, Tansu; Finkbeiner, Douglas; Lee, Albert; Meisner, Aaron; Schlegel, David; Valdes, Francisco

2018-01-01

The DECam Plane Survey is a five-band optical and near-infrared survey of the southern Galactic plane with the Dark Energy Camera at Cerro Tololo. The survey is designed to reach past the main-sequence turn-off at the distance of the Galactic center through a reddening E(B-V) of 1.5 mag. Typical single-exposure depths are 23.7, 22.8, 22.3, 21.9, and 21.0 mag in the grizY bands, with seeing around 1 arcsecond. The footprint covers the Galactic plane with |b| < 4°, 5° > l > -120°. The survey pipeline simultaneously solves for the positions and fluxes of tens of thousands of sources in each image, delivering positions and fluxes of roughly two billion stars with better than 10 mmag precision. Most of these objects are highly reddened and deep in the Galactic disk, probing the structure and properties of the Milky Way and its interstellar medium. The full survey is publicly available.

Photometric Calibrations of Gemini Images of NGC 6253

NASA Astrophysics Data System (ADS)

Pearce, Sean; Jeffery, Elizabeth

2017-01-01

We present preliminary results of our analysis of the metal-rich open cluster NGC 6253 using imaging data from GMOS on the Gemini-South Observatory. These data are part of a larger project to observe the effects of high metallicity on white dwarf cooling processes, especially the white dwarf cooling age, which have important implications on the processes of stellar evolution. To standardize the Gemini photometry, we have also secured imaging data of both the cluster and standard star fields using the 0.6-m SARA Observatory at CTIO. By analyzing and comparing the standard star fields of both the SARA data and the published Gemini zero-points of the standard star fields, we will calibrate the data obtained for the cluster. These calibrations are an important part of the project to obtain a standardized deep color-magnitude diagram to analyze the cluster. We present the process of verifying our standardization process. With a standardized CMD, we also present an analysis of the cluster's main sequence turn off age.

78 FR 67026 - Special Local Regulations; Recurring Marine Events in the Seventh Coast Guard District

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-08

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Stellar Variability at the Main-sequence Turnoff of the Intermediate-age LMC Cluster NGC 1846

NASA Astrophysics Data System (ADS)

Salinas, R.; Pajkos, M. A.; Vivas, A. K.; Strader, J.; Contreras Ramos, R.

2018-04-01

Intermediate-age (IA) star clusters in the Large Magellanic Cloud (LMC) present extended main-sequence turn-offs (MSTO) that have been attributed to either multiple stellar populations or an effect of stellar rotation. Recently it has been proposed that these extended main sequences can also be produced by ill-characterized stellar variability. Here we present Gemini-S/Gemini Multi-Object Spectrometer (GMOS) time series observations of the IA cluster NGC 1846. Using differential image analysis, we identified 73 new variable stars, with 55 of those being of the Delta Scuti type, that is, pulsating variables close the MSTO for the cluster age. Considering completeness and background contamination effects, we estimate the number of δ Sct belonging to the cluster between 40 and 60 members, although this number is based on the detection of a single δ Sct within the cluster half-light radius. This amount of variable stars at the MSTO level will not produce significant broadening of the MSTO, albeit higher-resolution imaging will be needed to rule out variable stars as a major contributor to the extended MSTO phenomenon. Though modest, this amount of δ Sct makes NGC 1846 the star cluster with the highest number of these variables ever discovered. Lastly, our results present a cautionary tale about the adequacy of shallow variability surveys in the LMC (like OGLE) to derive properties of its δ Sct population. Based on observations obtained at the Gemini Observatory, which is operated by the Association of Universities for Research in Astronomy, Inc., under a cooperative agreement with the NSF on behalf of the Gemini partnership: the National Science Foundation (United States), the National Research Council (Canada), CONICYT (Chile), Ministerio de Ciencia, Tecnología e Innovación Productiva (Argentina), and Ministério da Ciência, Tecnologia e Inovação (Brazil).

Super Gate Turn-Off Thyristor

DTIC Science & Technology

2006-08-01

semiconductor (nMOS) and turned it off through a positive metal oxide semiconductor (pMOS). For turn-on, although 1 V worked, a HP6227B power supply at 2...E3614A power supply at –8 V provided IG during the rise time, and thus need enough capacitance working to a frequency around 3/trise time...load’s screw terminal posts would have reduced the ESL and ESR. The SGTO turned off 5.8 A from the power supply at 20 V and was usually fan cooled

Novel control system of the high-voltage IGBT-switch

NASA Astrophysics Data System (ADS)

Ponomarev, A. V.; Mamontov, Y. I.; Gusev, A. I.; Pedos, M. S.

2017-05-01

HV solid-state switch control circuit was developed and tested. The switch was made with series connection IGBT-transistors. The distinctive feature of the circuit is an ability to fine-tune the switching time of every transistor. Simultaneous switching provides balancing of the dynamic voltage at all switch elements. A separate control board switches on and off every transistor. On and off signals from the main conductor are sent to the board by current pulses of different polarity. A positive pulse provides the transistor switch-on, while a negative pulse provides their switch-off. The time interval between pulses defines the time when the switch is turned on. The minimum time when the switch is turned on equals to a few microseconds, while the maximum time is not limited. This paper shows the test results of 4 kV switch prototype. The switch was used to produce rectangular pulses of a microsecond range under resistive load. The possibility to generate the damped harmonic oscillations was also tested. On the basis of this approach, positive testing results open up a possibility to design switches under an operating voltage of tens kilovolts.

Extended Star Formation or a Range of Stellar Rotation Velocities? The Nature of Extended Main Sequence Turnoffs in Intermediate-Age Star Clusters

NASA Astrophysics Data System (ADS)

Goudfrooij, Paul

2016-10-01

Recently, deep color-magnitude diagrams (CMDs) from HST data revealed that several massive intermediate-age star clusters in the Magellanic Clouds exhibit extended main-sequence turn-offs (eMSTOs), and in some cases also dual red clumps. This poses serious questions regarding the mechanisms responsible for the formation of massive star clusters and their well-known light-element abundance variations. The nature of eMSTOs is currently a hotly debated topic of study. Several recent studies indicate that the eMSTOs are caused by an age spread of about 100-500 Myr among cluster stars, while other studies indicate that eMSTOs can be caused by a coeval population in which the relevant stars span a range of rotation velocities. Formal evidence to (dis-)prove either scenario still remains at large, mainly because the available stellar tracks that incorporate the effects of rotation are only available for masses > 1.7 Msun whereas the stars in the known eMSTOs of intermediate-age clusters are less massive. To circumvent this issue, we identified a massive star cluster in the Large Magellanic Cloud (LMC) that has the right dynamical properties to host an eMSTO along with an age at which the effects of age spreads to CMD morphology are substantially different from those of spreads of rotation rates: the 600 Myr old cluster NGC 1831. We propose to obtain deep WFC3/UVIS imaging with filters F336W and F814W to analyze the morphologies of the MSTO and upper MS regions of NGC 1831 at high precision and compare with model predictions. This will have a lasting impact on our understanding of the eMSTO phenomenon and of star cluster formation in general.

Aptamer-mediated 'turn-off/turn-on' nanozyme activity of gold nanoparticles for kanamycin detection.

PubMed

Sharma, Tarun Kumar; Ramanathan, Rajesh; Weerathunge, Pabudi; Mohammadtaheri, Mahsa; Daima, Hemant Kumar; Shukla, Ravi; Bansal, Vipul

2014-12-28

A new ultrafast and highly sensitive 'turn-off/turn-on' biosensing approach that combines the intrinsic peroxidase-like activity of gold nanoparticles (GNPs) with the high affinity and specificity of a ssDNA aptamer is presented for the efficient detection of a model small molecule kanamycin.

Simulation and analysis of the effect of ungrounded rectangular loop distributed parameters on TEM response

NASA Astrophysics Data System (ADS)

Shi, Zongyang; Liu, Lihua; Xiao, Pan; Geng, Zhi; Liu, Fubo; Fang, Guangyou

2018-02-01

An ungrounded loop in the shallow subsurface transient electromagnetic surveys has been studied as the transmission line model for early turn-off stage, which can accurately explicate the early turn-off current waveform inconsistency along the loop. In this paper, the Gauss-Legendre numerical integration method is proposed for the first time to simulate and analyze the transient electromagnetic (TEM) response considering the different early turn-off current waveforms along the loop. During the simulation, these integral node positions along the loop are firstly determined by solving these zero points of Legendre polynomial, then the turn-off current of each node position is simulated by using the transfer function of the transmission line. Finally, the total TEM response is calculated by using the Gauss-Legendre integral formula. In addition, the comparison and analysis between the results affected by the distributed parameters and that generated by lumped parameters are presented. It is found that the TEM responses agree well with each other after current is thoroughly switched off, while the transient responses in turn-off stage are completely different. It means that the position dependence of the early turn-off current should be introduced into the forward model during the early response data interpretation of the shallow TEM detection of the ungrounded loop. Furthermore, the TEM response simulations at four geometric symmetry points are made. It shows that early responses of different geometric symmetry points are also inconsistent. The research on the influence of turn-off current position dependence on the early response of geometric symmetry point is of great significance to guide the layout of the survey lines and the transmitter location.

Photometric Metallicities of the Small and Large Magellanic Clouds

NASA Astrophysics Data System (ADS)

Miller, Amy Elizabeth

2018-06-01

In the field of astronomy, the study of galaxies is vitally important to understanding the structure and evolution of the universe. Within the study of galaxies, of particular interest are the Small and Large Magellanic Clouds (SMC and LMC, respectively), two of the Milky Way’s closest and most massive satellite galaxies. Their close proximity make them ideal candidates for understanding astrophysical processes such as galaxy interactions. In order to fully understand the Magellanic Clouds, it is imperative that the metallicity of the clouds be mapped in detail. In order to accomplish this task, I will use data from the Survey of Magellanic Stellar History (SMASH) which is a deep, multi-band (ugriz) photometric survey of the Magellanic Clouds that contains approximately 400 million objects in 197 fully-calibrated fields. SMASH is an extensive and deep photometric data set that enables the full-scale study of the galactic structure in the Clouds. The SMASH u-band is sensitive to metallicity for main-sequence turn-off stars which we calibrate using SDSS spectroscopy in overlapping regions (mainly standard star fields). The final steps will be to make metallicity maps of the main bodies and peripheries of the LMC and SMC. Ultimately, these metallicity maps will help us trace out population gradients in the Clouds and uncover the origin of their very extended stellar peripheries.

Hydrogels constructed via self-assembly of beta-hairpin molecules

NASA Astrophysics Data System (ADS)

Ozbas, Bulent

There is a recent and growing interest in hydrogel materials that are formed via peptide self-assembly for tissue engineering applications. Peptide based materials are excellent candidates for diverse applications in biomedical field due to their responsive behavior and complex self-assembled structures. However, there is very limited information on the self-assembly and resultant network and mechanical properties of these types of hydrogels. The main goal of this dissertation is to investigate the self-assembly mechanism and viscoelastic properties of hydrogels that can be altered by changing solution conditions as well as the primary structure of the peptide. These hydrogels are formed via intramolecular folding and consequent self-assembly of 20 amino acid long beta-hairpin peptide molecules (Max1). The peptide molecules are locally amphiphilic with two linear strands of alternating hydrophobic valine and hydrophilic lysine amino acids connected with a Dproline-LProline turn sequence. Circular dichroism and FTIR spectroscopy show that at physiological conditions peptides are unfolded in the absence of salt. By raising the ionic strength of the solution electrostatic interactions between charged lysines are screened and the peptide arms are forced into a beta-sheet secondary structure stabilized by the turn sequence. These folded molecules intermolecularly assemble via hydrophobic collapse and hydrogen bonding into a three dimensional network. Folding and self-assembly of these molecules can also be triggered by increasing temperature and/or pH of the peptide solution. In addition, the random-coil to beta-sheet transition of the beta-hairpin peptides is pH and, with proper changes in the peptide sequence, thermally reversible. Rheological measurements demonstrate that the resultant supramolecular structure forms an elastic material, whose structure, and thus modulus, can be tuned by magnitude of the stimulus. Hydrogels recover their initial viscoelastic properties after cessation of high magnitude of strain due to the physically crosslinked network structure and strong inter-fibrillar interactions. These interactions can be turned off by either condensing anions or covalently attaching PEG chains on lysine-decorated fibrillar surfaces. TEM, SANS, and rheological data reveal that the elasticity arises from a network consisting of semiflexible fibrillar assemblies that are monodisperse in width. The experimental results are compared with scaling relationships developed for permanently crosslinked semiflexible biopolymer networks. (Abstract shortened by UMI.)

Beryllium abundances along the evolutionary sequence of the open cluster IC 4651 - A new test for hydrodynamical stellar models

NASA Astrophysics Data System (ADS)

Smiljanic, R.; Pasquini, L.; Charbonnel, C.; Lagarde, N.

2010-02-01

Context. Previous analyses of lithium abundances in main sequence and red giant stars have revealed the action of mixing mechanisms other than convection in stellar interiors. Beryllium abundances in stars with Li abundance determinations can offer valuable complementary information on the nature of these mechanisms. Aims: Our aim is to derive Be abundances along the whole evolutionary sequence of an open cluster. We focus on the well-studied open cluster IC 4651. These Be abundances are used with previously determined Li abundances, in the same sample stars, to investigate the mixing mechanisms in a range of stellar masses and evolutionary stages. Methods: Atmospheric parameters were adopted from a previous abundance analysis by the same authors. New Be abundances have been determined from high-resolution, high signal-to-noise UVES spectra using spectrum synthesis and model atmospheres. The careful synthetic modeling of the Be lines region is used to calculate reliable abundances in rapidly rotating stars. The observed behavior of Be and Li is compared to theoretical predictions from stellar models including rotation-induced mixing, internal gravity waves, atomic diffusion, and thermohaline mixing. Results: Beryllium is detected in all the main sequence and turn-off sample stars, both slow- and fast-rotating stars, including the Li-dip stars, but is not detected in the red giants. Confirming previous results, we find that the Li dip is also a Be dip, although the depletion of Be is more modest than for Li in the corresponding effective temperature range. For post-main-sequence stars, the Be dilution starts earlier within the Hertzsprung gap than expected from classical predictions, as does the Li dilution. A clear dispersion in the Be abundances is also observed. Theoretical stellar models including the hydrodynamical transport processes mentioned above are able to reproduce all the observed features well. These results show a good theoretical understanding of the Li and Be behavior along the color-magnitude diagram of this intermediate-age cluster for stars more massive than 1.2 M⊙. Based on observations made with the ESO VLT, at Paranal Observatory, under programs 065.L-0427 and 067.D-0126.Current address: European Southern Observatory, Karl-Schwarzschild-Str. 2, 85748 Garching bei München, Germany.

Structural and sequence features of two residue turns in beta-hairpins.

PubMed

Madan, Bharat; Seo, Sung Yong; Lee, Sun-Gu

2014-09-01

Beta-turns in beta-hairpins have been implicated as important sites in protein folding. In particular, two residue β-turns, the most abundant connecting elements in beta-hairpins, have been a major target for engineering protein stability and folding. In this study, we attempted to investigate and update the structural and sequence properties of two residue turns in beta-hairpins with a large data set. For this, 3977 beta-turns were extracted from 2394 nonhomologous protein chains and analyzed. First, the distribution, dihedral angles and twists of two residue turn types were determined, and compared with previous data. The trend of turn type occurrence and most structural features of the turn types were similar to previous results, but for the first time Type II turns in beta-hairpins were identified. Second, sequence motifs for the turn types were devised based on amino acid positional potentials of two-residue turns, and their distributions were examined. From this study, we could identify code-like sequence motifs for the two residue beta-turn types. Finally, structural and sequence properties of beta-strands in the beta-hairpins were analyzed, which revealed that the beta-strands showed no specific sequence and structural patterns for turn types. The analytical results in this study are expected to be a reference in the engineering or design of beta-hairpin turn structures and sequences. © 2014 Wiley Periodicals, Inc.

Rail-to-rail differential input amplification stage with main and surrogate differential pairs

DOEpatents

Britton, Jr., Charles Lanier; Smith, Stephen Fulton

2007-03-06

An operational amplifier input stage provides a symmetrical rail-to-rail input common-mode voltage without turning off either pair of complementary differential input transistors. Secondary, or surrogate, transistor pairs assume the function of the complementary differential transistors. The circuit also maintains essentially constant transconductance, constant slew rate, and constant signal-path supply current as it provides rail-to-rail operation.

A novel high performance SemiSJ-CSTBT with p-pillar under the bottom of the trench gate

NASA Astrophysics Data System (ADS)

Yan, Jia; Hong, Chen; Ji, Tan; Shuojin, Lu; Yangjun, Zhu

2016-08-01

A novel high performance SemiSJ-CSTBT is proposed with the p-pillar under the bottom of the trench gate. The inserted p-pillar with the neighbouring n-drift region forms a lateral P/N junction, which can adjust the electric distribution in the forward-blocking mode to achieve a higher breakdown voltage compared to the conventional CSTBT. Also, the p-pillar can act as a hole collector at turn-off, which significantly enhances the turn-off speed and obtains a lower turn-off switching loss. Although the turn-off switching loss decreases as the depth of the p-pillar increases, there is no need for a very deep p-pillar. The associated voltage overshoot at turn-off increases dramatically with increasing the depth of p-pillar, which may cause destruction of the devices. Plus, this will add difficulty and cost to the manufacturing process of this new structure. Therefore, the proposed SemiSJ-CSTBT offers considerably better robustness compared to the conventional CSTBT and SJ-CSTBT. The simulation results show that the SemiSJ-CSTBT exhibits an increase in breakdown voltage by 160 V (13%) and a reduction of turn-off switching loss by approximately 15%. Project supported by the National Major Science and Technology Special Project of China (No. 2013ZX02305005-002) and the Major Program of the National Natural Science Foundation of China (No. 51490681).

Advances in traction drive technology

NASA Technical Reports Server (NTRS)

Loewenthal, S. H.; Anderson, N. E.; Rohn, D. A.

1983-01-01

Traction drives are traced from early uses as main transmissions in automobiles at the turn of the century to modern, high-powered traction drives capable of transmitting hundreds of horsepower. Recent advances in technology are described which enable today's traction drive to be a serious candidate for off-highway vehicles and helicopter applications. Improvements in materials, traction fluids, design techniques, power loss and life prediction methods will be highlighted. Performance characteristics of the Nasvytis fixed-ratio drive are given. Promising future drive applications, such as helicopter main transmissions and servo-control positioning mechanisms are also addressed.

Method for high resolution magnetic resonance analysis using magic angle technique

DOEpatents

Wind, Robert A.; Hu, Jian Zhi

2003-11-25

A method of performing a magnetic resonance analysis of a biological object that includes placing the biological object in a main magnetic field and in a radio frequency field, the main magnetic field having a static field direction; rotating the biological object at a rotational frequency of less than about 100 Hz around an axis positioned at an angle of about 54.degree.44' relative to the main magnetic static field direction; pulsing the radio frequency to provide a sequence that includes a magic angle turning pulse segment; and collecting data generated by the pulsed radio frequency. According to another embodiment, the radio frequency is pulsed to provide a sequence capable of producing a spectrum that is substantially free of spinning sideband peaks.

Fluorescent "on-off-on" switching sensor based on CdTe quantum dots coupled with multiwalled carbon nanotubes@graphene oxide nanoribbons for simultaneous monitoring of dual foreign DNAs in transgenic soybean.

PubMed

Li, Yaqi; Sun, Li; Qian, Jing; Long, Lingliang; Li, Henan; Liu, Qian; Cai, Jianrong; Wang, Kun

2017-06-15

With the increasing concern of potential health and environmental risk, it is essential to develop reliable methods for transgenic soybean detection. Herein, a simple, sensitive and selective assay was constructed based on homogeneous fluorescence resonance energy transfer (FRET) between CdTe quantum dots (QDs) and multiwalled carbon nanotubes@graphene oxide nanoribbons (MWCNTs@GONRs) to form the fluorescent "on-off-on" switching for simultaneous monitoring dual target DNAs of promoter cauliflower mosaic virus 35s (P35s) and terminator nopaline synthase (TNOS) from transgenic soybean. The capture DNAs were immobilized with corresponding QDs to obtain strong fluorescent signals (turning on). The strong π-π stacking interaction between single-stranded DNA (ssDNA) probes and MWCNTs@GONRs led to minimal background fluorescence due to the FRET process (turning off). The targets of P35s and TNOS were recognized by dual fluorescent probes to form double-stranded DNA (dsDNA) through the specific hybridization between target DNAs and ssDNA probes. And the dsDNA were released from the surface of MWCNTs@GONRs, which leaded the dual fluorescent probes to generate the strong fluorescent emissions (turning on). Therefore, this proposed homogeneous assay can be achieved to detect P35s and TNOS simultaneously by monitoring the relevant fluorescent emissions. Moreover, this assay can distinguish complementary and mismatched nucleic acid sequences with high sensitivity. The constructed approach has the potential to be a tool for daily detection of genetically modified organism with the merits of feasibility and reliability. Copyright © 2017 Elsevier B.V. All rights reserved.

The zero age main sequence of WIMP burners

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fairbairn, Malcolm; Scott, Pat; Edsjoe, Joakim

2008-02-15

We modify a stellar structure code to estimate the effect upon the main sequence of the accretion of weakly-interacting dark matter onto stars and its subsequent annihilation. The effect upon the stars depends upon whether the energy generation rate from dark matter annihilation is large enough to shut off the nuclear burning in the star. Main sequence weakly-interacting massive particles (WIMP) burners look much like proto-stars moving on the Hayashi track, although they are in principle completely stable. We make some brief comments about where such stars could be found, how they might be observed and more detailed simulations whichmore » are currently in progress. Finally we comment on whether or not it is possible to link the paradoxically hot, young stars found at the galactic center with WIMP burners.« less

Dynamical age differences among coeval star clusters as revealed by blue stragglers.

PubMed

Ferraro, F R; Lanzoni, B; Dalessandro, E; Beccari, G; Pasquato, M; Miocchi, P; Rood, R T; Sigurdsson, S; Sills, A; Vesperini, E; Mapelli, M; Contreras, R; Sanna, N; Mucciarelli, A

2012-12-20

Globular star clusters that formed at the same cosmic time may have evolved rather differently from the dynamical point of view (because that evolution depends on the internal environment) through a variety of processes that tend progressively to segregate stars more massive than the average towards the cluster centre. Therefore clusters with the same chronological age may have reached quite different stages of their dynamical history (that is, they may have different 'dynamical ages'). Blue straggler stars have masses greater than those at the turn-off point on the main sequence and therefore must be the result of either a collision or a mass-transfer event. Because they are among the most massive and luminous objects in old clusters, they can be used as test particles with which to probe dynamical evolution. Here we report that globular clusters can be grouped into a few distinct families on the basis of the radial distribution of blue stragglers. This grouping corresponds well to an effective ranking of the dynamical stage reached by stellar systems, thereby permitting a direct measure of the cluster dynamical age purely from observed properties.

The origin of the LMC stellar bar: clues from the SFH of the bar and inner disc

NASA Astrophysics Data System (ADS)

Monteagudo, L.; Gallart, C.; Monelli, M.; Bernard, E. J.; Stetson, P. B.

2018-01-01

We discuss the origin of the Large Magellanic Cloud (LMC) stellar bar by comparing the star formation histories (SFHs) obtained from deep colour-magnitude diagrams (CMDs) in the bar and in a number of fields in different directions within the inner disc. The CMDs, reaching the oldest main-sequence turn-offs in these very crowded fields, have been obtained with VIMOS on the Very Large Telescope in the service mode, under very good seeing conditions. We show that the SFHs of all fields share the same patterns, with consistent variations of the star formation rate as a function of time in all of them. We therefore conclude that no specific event of star formation can be identified with the formation of the LMC bar, which instead likely formed from a redistribution of disc material which occurred when the LMC disc became bar unstable, and shared a common SFH with the inner disc thereafter. The strong similarity between the SFH of the centre and edge of the bar rules out the expected significant spatial variations of the SFH across the bar.

Cresting the wave: proper motions of the Eastern Banded Structure

NASA Astrophysics Data System (ADS)

Deason, Alis J.; Belokurov, Vasily; Koposov, Sergey E.

2018-01-01

We study the kinematic properties of the Eastern Banded Structure (EBS) and Hydra I overdensity using exquisite proper motions derived from the Sloan Digital Sky Survey (SDSS) and Gaia source catalogue. Main sequence turn-off stars in the vicinity of the EBS are identified from SDSS photometry; we use the proper motions and, where applicable, spectroscopic measurements of these stars to probe the kinematics of this apparent stream. We find that the EBS and Hydra I share common kinematic and chemical properties with the nearby Monoceros Ring. In particular, the proper motions of the EBS, like Monoceros, are indicative of prograde rotation (Vϕ ∼ 180-220 km s-1), which is similar to the Galactic thick disc. The kinematic structure of stars in the vicinity of the EBS suggests that it is not a distinct stellar stream, but rather marks the 'edge' of the Monoceros Ring. The EBS and Hydra I are the latest substructures to be linked with Monoceros, leaving the Galactic anti-centre a mess of interlinked overdensities which likely share a unified, Galactic disc origin.

The DECam Plane Survey: Optical Photometry of Two Billion Objects in the Southern Galactic Plane

NASA Astrophysics Data System (ADS)

Schlafly, E. F.; Green, G. M.; Lang, D.; Daylan, T.; Finkbeiner, D. P.; Lee, A.; Meisner, A. M.; Schlegel, D.; Valdes, F.

2018-02-01

The DECam Plane Survey is a five-band optical and near-infrared survey of the southern Galactic plane with the Dark Energy Camera at Cerro Tololo. The survey is designed to reach past the main-sequence turn-off of old populations at the distance of the Galactic center through a reddening E(B-V) of 1.5 mag. Typical single-exposure depths are 23.7, 22.8, 22.3, 21.9, and 21.0 mag (AB) in the grizY bands, with seeing around 1\\prime\\prime . The footprint covers the Galactic plane with | b| ≲ 4^\\circ , 5^\\circ > l> -120^\\circ . The survey pipeline simultaneously solves for the positions and fluxes of tens of thousands of sources in each image, delivering positions and fluxes of roughly two billion stars with better than 10 mmag precision. Most of these objects are highly reddened and deep in the Galactic disk, probing the structure and properties of the Milky Way and its interstellar medium. The fully-processed images and derived catalogs are publicly available.

"Turn-off" fluorescent data array sensor based on double quantum dots coupled with chemometrics for highly sensitive and selective detection of multicomponent pesticides.

PubMed

Fan, Yao; Liu, Li; Sun, Donglei; Lan, Hanyue; Fu, Haiyan; Yang, Tianming; She, Yuanbin; Ni, Chuang

2016-04-15

As a popular detection model, the fluorescence "turn-off" sensor based on quantum dots (QDs) has already been successfully employed in the detections of many materials, especially in the researches on the interactions between pesticides. However, the previous studies are mainly focused on simple single track or the comparison based on similar concentration of drugs. In this work, a new detection method based on the fluorescence "turn-off" model with water-soluble ZnCdSe and CdSe QDs simultaneously as the fluorescent probes is established to detect various pesticides. The fluorescence of the two QDs can be quenched by different pesticides with varying degrees, which leads to the differences in positions and intensities of two peaks. By combining with chemometrics methods, all the pesticides can be qualitative and quantitative respectively even in real samples with the limit of detection was 2 × 10(-8) mol L(-1) and a recognition rate of 100%. This work is, to the best of our knowledge, the first report on the detection of pesticides based on the fluorescence quenching phenomenon of double quantum dots combined with chemometrics methods. What's more, the excellent selectivity of the system has been verified in different mediums such as mixed ion disruption, waste water, tea and water extraction liquid drugs. Copyright © 2016 Elsevier B.V. All rights reserved.

Protein structure prediction with local adjust tabu search algorithm

PubMed Central

2014-01-01

Background Protein folding structure prediction is one of the most challenging problems in the bioinformatics domain. Because of the complexity of the realistic protein structure, the simplified structure model and the computational method should be adopted in the research. The AB off-lattice model is one of the simplification models, which only considers two classes of amino acids, hydrophobic (A) residues and hydrophilic (B) residues. Results The main work of this paper is to discuss how to optimize the lowest energy configurations in 2D off-lattice model and 3D off-lattice model by using Fibonacci sequences and real protein sequences. In order to avoid falling into local minimum and faster convergence to the global minimum, we introduce a novel method (SATS) to the protein structure problem, which combines simulated annealing algorithm and tabu search algorithm. Various strategies, such as the new encoding strategy, the adaptive neighborhood generation strategy and the local adjustment strategy, are adopted successfully for high-speed searching the optimal conformation corresponds to the lowest energy of the protein sequences. Experimental results show that some of the results obtained by the improved SATS are better than those reported in previous literatures, and we can sure that the lowest energy folding state for short Fibonacci sequences have been found. Conclusions Although the off-lattice models is not very realistic, they can reflect some important characteristics of the realistic protein. It can be found that 3D off-lattice model is more like native folding structure of the realistic protein than 2D off-lattice model. In addition, compared with some previous researches, the proposed hybrid algorithm can more effectively and more quickly search the spatial folding structure of a protein chain. PMID:25474708

Development of a 1000V, 200A, low-loss, fast-switching, gate-assisted turn-off thyristor

NASA Technical Reports Server (NTRS)

Schlegel, E. S.; Lowry, L. R.; Moore, D. L.

1977-01-01

The results of a program to develop a fast high power thyristor that can operate in switching circuits at frequencies of 10 to 20 kHz with very low power loss are given. Feasibility was demonstrated for a thyristor that blocks 1000V forward and reverse, conducts 200A, turns on in little more than 2 more microseconds with only 2A of gate drive, turns off in 3 microseconds with 2A of gate assist current and has an energy dissipation of only 12 mJ per pulse for a 20 microsecond half sine wave 200A pulse. Data were generated that clearly showed the tradeoffs that can be made between the turn off time and forward drop. The understanding of this relationship is necessary in the selection of deliverable thyristors with turn off times up to 7 microseconds to give improved efficiency in a series resonant dc to dc inverter application.

ARL Eye Safer Fiber Laser Testbed Lab View Automation and Control

DTIC Science & Technology

2013-09-01

output voltage value in volts. gpc n Program the output current value in amperes. grst Reset and bring the power supplies to safe state. gout n...Turn the output on/off: gout 1 = turn on, gout 0 = turn off Figure 4 shows the front panel of power supplies and back panel RS 485 link. 4

14 CFR 91.609 - Flight data recorders and cockpit voice recorders.

Code of Federal Regulations, 2010 CFR

2010-01-01

... recorder or cockpit voice recorder is turned off to test it or to test any communications or electrical... recorder or cockpit voice recorder is turned off to test it or to test any communications or electrical... continuously from the instant the airplane begins the takeoff roll or the rotorcraft begins lift-off until the...

Network Level Association and Fusion of Kinematic and Attribute Information

DTIC Science & Technology

2010-12-15

L. Svensson, ``More Ways to Track Closely-Spaced Targets Than You Wanted To Know or Tips For Not Pissing Off the Radar Operator So Much That He Turns...To Know or Tips For Not Pissing Off the Radar Operator So Much That He Turns Off the Track Display”, Proc. ONR-GTRI Workshop on Tracking, Santa Barbara

Cutting the Cord

NASA Technical Reports Server (NTRS)

2004-01-01

This animation shows the view from the front hazard avoidance cameras on the Mars Exploration Rover Spirit as the rover turns 45 degrees clockwise. This maneuver is the first step in a 3-point turn that will rotate the rover 115 degrees to face west. The rover must make this turn before rolling off the lander because airbags are blocking it from exiting off the front lander petal. Before this crucial turn could take place, engineers instructed the rover to cut the final cord linking it to the lander. The turn took around 30 minutes to complete.

Effect of transmitter turn-off time on transient soundings

USGS Publications Warehouse

Fitterman, D.V.; Anderson, W.L.

1987-01-01

A general procedure for computing the effect of non-zero turn-off time on the transient electromagnetic response is presented which can be applied to forward and inverse calculation methods for any transmitter-receiver configuration. We consider in detail the case of a large transmitter loop which has a receiver coil located at the center of the loop (central induction or in-loop array). For a linear turn-off ramp of width t0, the voltage response is shown to be the voltage due to an ideal step turn-off averaged over windows of width t0. Thus the effect is similar to that obtained by using averaging windows in the receiver. In general when time zero is taken to be the end of the ramp, the apparent resistivity increases for a homogeneous half-space over a limited time range. For time zero taken to be the start of the ramp the apparent resistivity is affected in the opposite direction. The effect of the ramp increases with increasing t0 and first-layer resistivity, is largest during the intermediate stage, and decreases with increasing time. It is shown that for a ramp turn-off, there is no effect in the early and late stages. For two-layered models with a resistive first layer (??1>??2), the apparent resistivity is increased in the intermediate stage. When the first layer is more conductive than the second layer (??1

Silicon Carbide Emitter Turn-Off Thyristor

DOE PAGES

Wang, Jun; Wang, Gangyao; Li, Jun; ...

2008-01-01

A novel MOS-conmore » trolled SiC thyristor device, the SiC emitter turn-off thyristor (ETO) is a promising technology for future high-voltage switching applications because it integrates the excellent current conduction capability of a SiC thyristor with a simple MOS-control interface. Through unity-gain turn-off, the SiC ETO also achieves excellent Safe Operation Area (SOA) and faster switching speeds than silicon ETOs. The world's first 4.5-kV SiC ETO prototype shows a forward voltage drop of 4.26 V at 26.5  A / cm 2 current density at room and elevated temperatures. Tested in an inductive circuit with a 2.5 kV DC link voltage and a 9.56-A load current, the SiC ETO shows a fast turn-off time of 1.63 microseconds and a low 9.88 mJ turn-off energy. The low switching loss indicates that the SiC ETO could operate at about 4 kHz if 100  W / cm 2 conduction and the 100  W / cm 2 turn-off losses can be removed by the thermal management system. This frequency capability is about 4 times higher than 4.5-kV-class silicon power devices. The preliminary demonstration shows that the SiC ETO is a promising candidate for high-frequency, high-voltage power conversion applications, and additional developments to optimize the device for higher voltage (>5 kV) and higher frequency (10 kHz) are needed.« less

On the Use of the Main-sequence Knee (Saddle) to Measure Globular Cluster Ages

NASA Astrophysics Data System (ADS)

Saracino, S.; Dalessandro, E.; Ferraro, F. R.; Lanzoni, B.; Origlia, L.; Salaris, M.; Pietrinferni, A.; Geisler, D.; Kalirai, J. S.; Correnti, M.; Cohen, R. E.; Mauro, F.; Villanova, S.; Moni Bidin, C.

2018-06-01

In this paper, we review the operational definition of the so-called main-sequence knee (MS-knee), a feature in the color-magnitude diagram (CMD) occurring at the low-mass end of the MS. The magnitude of this feature is predicted to be independent of age at fixed chemical composition. For this reason, its difference in magnitude with respect to the MS turn-off (MS-TO) point has been suggested as a possible diagnostic to estimate absolute globular cluster (GC) ages. We first demonstrate that the operational definition of the MS-knee currently adopted in the literature refers to the inflection point of the MS (which we here more appropriately named MS-saddle), a feature that is well distinct from the knee and which cannot be used as its proxy. The MS-knee is only visible in near-infrared CMDs, while the MS-saddle can be also detected in optical–NIR CMDs. By using different sets of isochrones, we then demonstrate that the absolute magnitude of the MS-knee varies by a few tenths of a dex from one model to another, thus showing that at the moment stellar models may not capture the full systematic error in the method. We also demonstrate that while the absolute magnitude of the MS-saddle is almost coincident in different models, it has a systematic dependence on the adopted color combinations which is not predicted by stellar models. Hence, it cannot be used as a reliable reference for absolute age determination. Moreover, when statistical and systematic uncertainties are properly taken into account, the difference in magnitude between the MS-TO and the MS-saddle does not provide absolute ages with better accuracy than other methods like the MS-fitting.

40 CFR Appendix A to Subpart T of... - Test of Solvent Cleaning Procedures

Code of Federal Regulations, 2011 CFR

2011-07-01

... condenser or the sump heater? A. Primary condenser B. Sump heater C. Turn both on at same time D. Either A or B is correct ___ 8. During shutdown, what must be turned off first, the primary condenser or the sump heater? A. Primary condenser B. Sump heater C. Turn both off at same time D. Either A or B is...

Testing of a Methane Cryogenic Heat Pipe with a Liquid Trap Turn-Off Feature for use on Space Interferometer Mission (SIM)

NASA Technical Reports Server (NTRS)

Cepeda-Rizo, Juan; Krylo, Robert; Fisher, Melanie; Bugby, David C.

2011-01-01

Camera cooling for SIM presents three thermal control challenges; stable operation at 163K (110 C), decontamination heating to +20 C, and a long span from the cameras to the radiator. A novel cryogenic cooling system based on a methane heat pipe meets these challenges. The SIM thermal team, with the help of heat pipe vendor ATK, designed and tested a complete, low temperature, cooling system. The system accommodates the two SIM cameras with a double-ended conduction bar, a single methane heat pipe, independent turn-off devices, and a flight-like radiator. The turn ]off devices consist of a liquid trap, for removing the methane from the pipe, and an electrical heater to raise the methane temperature above the critical point thus preventing two-phase operation. This is the first time a cryogenic heat pipe has been tested at JPL and is also the first heat pipe to incorporate the turn-off features. Operation at 163K with a methane heat pipe is an important new thermal control capability for the lab. In addition, the two turn-off technologies enhance the "bag of tricks" available to the JPL thermal community. The successful test program brings this heat pipe to a high level of technology readiness.

Eu3+ complex of ligand4’-(4-carboxyphenyl)-2,2’:6’,2″-terpyridine as fluorosensor of heavy metals

NASA Astrophysics Data System (ADS)

Zulys, A.; Rachmawati, N.

2017-04-01

Ligand 4’-(4-carboxyphenyl)-2,2’:6’,2″-terpyridine (2-Hcptpy) has been synthesized by modification of Kröhnke method. The synthesize was performed using aldol condensation reaction. The white precipitate was collected and produced 62% yield (1.062 gr). Ligand 2-Hcptpy has been characterized by FTIR, Elemental analyzer, H-NMR, UV-vis, and UV-DRS spectrometer. It was then reacted with lanthanide group (Eu3+) to form a complex by hydrothermal process. The result of Eu3+ complex was 0.352 gr. of white yellowish precipitate. The application of this research is for the fluorosensor of heavy metals (Pb2+dan Cd2+). The data of fluorescence showed two types of fluorescence, either turn on or turn off fluorosensor. Ligand 2-Hcptpy has an on-off type with the addition of Pb2+ and Cd2+, while complex Eu3+ has two types of fluorosensor. The complex showed turn on-off and turn on-off by addition of Pb2+ and type of turn off by addition of Cd2+. Either ligand or complex, showed fluorescence intensity by adding heavy metals up to concentration 5×10-8 M.

Controller for thermostatically controlled loads

DOEpatents

Lu, Ning; Zhang, Yu; Du, Pengwei; Makarov, Yuri V.

2016-06-07

A system and method of controlling aggregated thermostatically controlled appliances (TCAs) for demand response is disclosed. A targeted load profile is formulated and a forecasted load profile is generated. The TCAs within an "on" or "off" control group are prioritized based on their operating temperatures. The "on" or "off" status of the TCAs is determined. Command signals are sent to turn on or turn off the TCAs.

Surface friction alters the agility of a small Australian marsupial.

PubMed

Wheatley, Rebecca; Clemente, Christofer J; Niehaus, Amanda C; Fisher, Diana O; Wilson, Robbie S

2018-04-23

Movement speed can underpin an animal's probability of success in ecological tasks. Prey often use agility to outmanoeuvre predators; however, faster speeds increase inertia and reduce agility. Agility is also constrained by grip, as the foot must have sufficient friction with the ground to apply the forces required for turning. Consequently, ground surface should affect optimum turning speed. We tested the speed-agility trade-off in buff-footed antechinus ( Antechinus mysticus ) on two different surfaces. Antechinus used slower turning speeds over smaller turning radii on both surfaces, as predicted by the speed-agility trade-off. Slipping was 64% more likely on the low-friction surface, and had a higher probability of occurring the faster the antechinus were running before the turn. However, antechinus compensated for differences in surface friction by using slower pre-turn speeds as their amount of experience on the low-friction surface increased, which consequently reduced their probability of slipping. Conversely, on the high-friction surface, antechinus used faster pre-turn speeds in later trials, which had no effect on their probability of slipping. Overall, antechinus used larger turning radii (0.733±0.062 versus 0.576±0.051 m) and slower pre-turn (1.595±0.058 versus 2.174±0.050 m s -1 ) and turning speeds (1.649±0.061 versus 2.01±0.054 m s -1 ) on the low-friction surface. Our results demonstrate the interactive effect of surface friction and the speed-agility trade-off on speed choice. To predict wild animals' movement speeds, future studies should examine the interactions between biomechanical trade-offs and terrain, and quantify the costs of motor mistakes in different ecological activities. © 2018. Published by The Company of Biologists Ltd.

"Turn-off" fluorescent sensor for highly sensitive and specific simultaneous recognition of 29 famous green teas based on quantum dots combined with chemometrics.

PubMed

Liu, Li; Fan, Yao; Fu, Haiyan; Chen, Feng; Ni, Chuang; Wang, Jinxing; Yin, Qiaobo; Mu, Qingling; Yang, Tianming; She, Yuanbin

2017-04-22

Fluorescent "turn-off" sensors based on water-soluble quantum dots (QDs) have drawn increasing attention owing to their unique properties such as high fluorescence quantum yields, chemical stability and low toxicity. In this work, a novel method based on the fluorescence "turn-off" model with water-soluble CdTe QDs as the fluorescent probes for differentiation of 29 different famous green teas is established. The fluorescence of the QDs can be quenched in different degrees in light of positions and intensities of the fluorescent peaks for the green teas. Subsequently, with aid of classic partial least square discriminant analysis (PLSDA), all the green teas can be discriminated with high sensitivity, specificity and a satisfactory recognition rate of 100% for training set and 98.3% for prediction set, respectively. Especially, the "turn-off" fluorescence PLSDA model based on second-order derivatives (2nd der) with reduced least complexity (LVs = 3) was the most effective one for modeling. Most importantly, we further demonstrated the established "turn-off" fluorescent sensor mode has several significant advantages and appealing properties over the conventional fluorescent method for large-class-number classification (LCNC) of green teas. This work is, to the best of our knowledge, the first report on the rapid and effective identification of so many kinds of famous green teas based on the "turn-off" model of QDs combined with chemometrics, which also implies other potential applications on complex LCNC classification system with weak fluorescence or even without fluorescence to achieve higher detective response and specificity. Copyright © 2017 Elsevier B.V. All rights reserved.

SEPP-ZVS High Frequency Inverter Incorporating Auxiliary Switch

NASA Astrophysics Data System (ADS)

Ogiwara, Hiroyuki; Itoi, Misao; Nakaoka, Mutsuo

This paper presents a novel circuit topology to attain ZVS operation of a high frequency inverter over a wide range output power regulation using a PWM control technique by connecting an auxiliary switch to the conventional single ended push-pull (SEPP) ZVS high frequency inverter. A switching current is injected into the main switches via the auxiliary switch only during the short period between its turn-on and off times to supply a current required for its ZVS operation.

Chemical and Biophysical Modulation of Cas9 for Tunable Genome Engineering.

PubMed

Nuñez, James K; Harrington, Lucas B; Doudna, Jennifer A

2016-03-18

The application of the CRISPR-Cas9 system for genome engineering has revolutionized the ability to interrogate genomes of mammalian cells. Programming the Cas9 endonuclease to induce DNA breaks at specified sites is achieved by simply modifying the sequence of its cognate guide RNA. Although Cas9-mediated genome editing has been shown to be highly specific, cleavage events at off-target sites have also been reported. Minimizing, and eventually abolishing, unwanted off-target cleavage remains a major goal of the CRISPR-Cas9 technology before its implementation for therapeutic use. Recent efforts have turned to chemical biology and biophysical approaches to engineer inducible genome editing systems for controlling Cas9 activity at the transcriptional and protein levels. Here, we review recent advancements to modulate Cas9-mediated genome editing by engineering split-Cas9 constructs, inteins, small molecules, protein-based dimerizing domains, and light-inducible systems.

Sequence swapping does not result in conformation swapping for the beta4/beta5 and beta8/beta9 beta-hairpin turns in human acidic fibroblast growth factor.

PubMed

Kim, Jaewon; Lee, Jihun; Brych, Stephen R; Logan, Timothy M; Blaber, Michael

2005-02-01

The beta-turn is the most common type of nonrepetitive structure in globular proteins, comprising ~25% of all residues; however, a detailed understanding of effects of specific residues upon beta-turn stability and conformation is lacking. Human acidic fibroblast growth factor (FGF-1) is a member of the beta-trefoil superfold and contains a total of five beta-hairpin structures (antiparallel beta-sheets connected by a reverse turn). beta-Turns related by the characteristic threefold structural symmetry of this superfold exhibit different primary structures, and in some cases, different secondary structures. As such, they represent a useful system with which to study the role that turn sequences play in determining structure, stability, and folding of the protein. Two turns related by the threefold structural symmetry, the beta4/beta5 and beta8/beta9 turns, were subjected to both sequence-swapping and poly-glycine substitution mutations, and the effects upon stability, folding, and structure were investigated. In the wild-type protein these turns are of identical length, but exhibit different conformations. These conformations were observed to be retained during sequence-swapping and glycine substitution mutagenesis. The results indicate that the beta-turn structure at these positions is not determined by the turn sequence. Structural analysis suggests that residues flanking the turn are a primary structural determinant of the conformation within the turn.

Defining the loop structures in proteins based on composite β-turn mimics.

PubMed

Dhar, Jesmita; Chakrabarti, Pinak

2015-06-01

Asx- and ω-turns are β-turn mimics, which replace the conventional main-chain hydrogen bonds seen in the latter by those involving the side chains, and both involve three residues. In this paper we analyzed the cases where these turns occur together--side by side, with or without any gap, overlapping and in any order. These composite turns (of length 3-15 residues), occurring at ∼1 per 100 residues, may constitute the full length of many loops, and when the residues in the two component turns overlap or are adjacent to each other, the composite may take well-defined shape. It is thus possible for non-regular regions in protein structure to form local structural motifs, akin to the regular geometrical features exhibited by secondary structures. Composites having the order ω-turns followed by Asx-turns can constitute N-terminal helix capping motif. Ternary composite turns (made up of ω-, Asx- and ST-turns), some with characteristic shape, have also been identified. Delineation of composite turns would help in characterizing loops in protein structures, which often have functional roles. Some sequence patterns seen in composites can be used for their incorporation in protein design. © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.

Adaptive efficient compression of genomes

PubMed Central

2012-01-01

Modern high-throughput sequencing technologies are able to generate DNA sequences at an ever increasing rate. In parallel to the decreasing experimental time and cost necessary to produce DNA sequences, computational requirements for analysis and storage of the sequences are steeply increasing. Compression is a key technology to deal with this challenge. Recently, referential compression schemes, storing only the differences between a to-be-compressed input and a known reference sequence, gained a lot of interest in this field. However, memory requirements of the current algorithms are high and run times often are slow. In this paper, we propose an adaptive, parallel and highly efficient referential sequence compression method which allows fine-tuning of the trade-off between required memory and compression speed. When using 12 MB of memory, our method is for human genomes on-par with the best previous algorithms in terms of compression ratio (400:1) and compression speed. In contrast, it compresses a complete human genome in just 11 seconds when provided with 9 GB of main memory, which is almost three times faster than the best competitor while using less main memory. PMID:23146997

Identifying Multiple Populations in M71 using CN

NASA Astrophysics Data System (ADS)

Gerber, Jeffrey M.; Friel, Eileen D.; Vesperini, Enrico

2018-01-01

It is now well established that globular clusters (GCs) host multiple stellar populations characterized by differences in several light elements. While these populations have been found in nearly all GCs, we still lack an entirely successful model to explain their formation. A key constraint to these models is the detailed pattern of light element abundances seen among the populations; different techniques for identifying these populations probe different elements and do not always yield the same results. We study a large sample of stars in the GC M71 for light elements C and N, using the CN and CH band strength to identify multiple populations. Our measurements come from low-resolution spectroscopy obtained with the WIYN-3.5m telescope for ~150 stars from the tip of the red-giant branch down to the main-sequence turn-off. The large number of stars and broad spatial coverage of our sample (out to ~3.5 half-light radii) allows us to carry out a comprehensive characterization of the multiple populations in M71. We use a combination of the various spectroscopic and photometric indicators to draw a more complete picture of the properties of the populations and to investigate the consistency of classifications using different techniques.

Decreased sleep stage transition pattern complexity in narcolepsy type 1.

PubMed

Ferri, Raffaele; Pizza, Fabio; Vandi, Stefano; Iloti, Martina; Plazzi, Giuseppe

2016-08-01

To analyze the complexity of the nocturnal sleep stage sequence in central disorders of hypersomnolence (CDH), with the hypothesis that narcolepsy type 1 (NT1) might exhibit distinctive sleep stage sequence organization and complexity. Seventy-nine NT1 patients, 22 narcolepsy type 2 (NT2), 22 idiopathic hypersomnia (IH), and 52 patients with subjective hypersomnolence (sHS) were recruited and their nocturnal sleep was polysomnographically recorded and scored. Group between-stage transition probability matrices were obtained and compared. Patients with NT1 differed significantly from all the other patient groups, the latter, in turn, were not different between each other. The individual probability of the R-to-N2 transition was found to be the parameter showing the difference of highest significance between the groups (lowest in NT1) and classified patients with or without NT1 with an accuracy of 78.9% (sensitivity 78.5% and specificity 79.2%), by applying a cut-off value of 0.15. The main result of this study is that the structure of the sleep stage transition pattern of hypocretin-deficient NT1 patients is significantly different from that of other forms of CDH and sHS, with normal hypocretin levels. The lower probability of R-to-N2 transition occurrence in NT1 appears to be a reliable polysomnographic feature with potential application at the individual level, for supportive diagnostic purposes. Copyright © 2016 International Federation of Clinical Neurophysiology. Published by Elsevier Ireland Ltd. All rights reserved.

Prediction of pi-turns in proteins using PSI-BLAST profiles and secondary structure information.

PubMed

Wang, Yan; Xue, Zhi-Dong; Shi, Xiao-Hong; Xu, Jin

2006-09-01

Due to the structural and functional importance of tight turns, some methods have been proposed to predict gamma-turns, beta-turns, and alpha-turns in proteins. In the past, studies of pi-turns were made, but not a single prediction approach has been developed so far. It will be useful to develop a method for identifying pi-turns in a protein sequence. In this paper, the support vector machine (SVM) method has been introduced to predict pi-turns from the amino acid sequence. The training and testing of this approach is performed with a newly collected data set of 640 non-homologous protein chains containing 1931 pi-turns. Different sequence encoding schemes have been explored in order to investigate their effects on the prediction performance. With multiple sequence alignment and predicted secondary structure, the final SVM model yields a Matthews correlation coefficient (MCC) of 0.556 by a 7-fold cross-validation. A web server implementing the prediction method is available at the following URL: http://210.42.106.80/piturn/.

Track Detection in Railway Sidings Based on MEMS Gyroscope Sensors

PubMed Central

Broquetas, Antoni; Comerón, Adolf; Gelonch, Antoni; Fuertes, Josep M.; Castro, J. Antonio; Felip, Damià; López, Miguel A.; Pulido, José A.

2012-01-01

The paper presents a two-step technique for real-time track detection in single-track railway sidings using low-cost MEMS gyroscopes. The objective is to reliably know the path the train has taken in a switch, diverted or main road, immediately after the train head leaves the switch. The signal delivered by the gyroscope is first processed by an adaptive low-pass filter that rejects noise and converts the temporal turn rate data in degree/second units into spatial turn rate data in degree/meter. The conversion is based on the travelled distance taken from odometer data. The filter is implemented to achieve a speed-dependent cut-off frequency to maximize the signal-to-noise ratio. Although direct comparison of the filtered turn rate signal with a predetermined threshold is possible, the paper shows that better detection performance can be achieved by processing the turn rate signal with a filter matched to the rail switch curvature parameters. Implementation aspects of the track detector have been optimized for real-time operation. The detector has been tested with both simulated data and real data acquired in railway campaigns. PMID:23443376

Sequential power-up circuit

DOEpatents

Kronberg, James W.

1992-01-01

A sequential power-up circuit for starting several electrical load elements in series to avoid excessive current surge, comprising a voltage ramp generator and a set of voltage comparators, each comparator having a different reference voltage and interfacing with a switch that is capable of turning on one of the load elements. As the voltage rises, it passes the reference voltages one at a time and causes the switch corresponding to that voltage to turn on its load element. The ramp is turned on and off by a single switch or by a logic-level electrical signal. The ramp rate for turning on the load element is relatively slow and the rate for turning the elements off is relatively fast. Optionally, the duration of each interval of time between the turning on of the load elements is programmable.

A statistical evaluation of effective time constants of random telegraph noise with various operation timings of in-pixel source follower transistors

NASA Astrophysics Data System (ADS)

Yonezawa, A.; Kuroda, R.; Teramoto, A.; Obara, T.; Sugawa, S.

2014-03-01

We evaluated effective time constants of random telegraph noise (RTN) with various operation timings of in-pixel source follower transistors statistically, and discuss the dependency of RTN time constants on the duty ratio (on/off ratio) of MOSFET which is controlled by the gate to source voltage (VGS). Under a general readout operation of CMOS image sensor (CIS), the row selected pixel-source followers (SFs) turn on and not selected pixel-SFs operate at different bias conditions depending on the select switch position; when select switch locate in between the SF driver and column output line, SF drivers nearly turn off. The duty ratio and cyclic period of selected time of SF driver depends on the operation timing determined by the column read out sequence. By changing the duty ratio from 1 to 7.6 x 10-3, time constant ratio of RTN (time to capture <τc<)/(time to emission <τe<) of a part of MOSFETs increased while RTN amplitudes were almost the same regardless of the duty ratio. In these MOSFETs, <τc< increased and the majority of <τe< decreased and the minority of <τe< increased by decreasing the duty ratio. The same tendencies of behaviors of <τc< and <τe< were obtained when VGS was decreased. This indicates that the effective <τc< and <τe< converge to those under off state as duty ratio decreases. These results are important for the noise reduction, detection and analysis of in pixel-SF with RTN.

Fast Turn-Off Times Observed in Experimental 4H SiC Thyristors

NASA Technical Reports Server (NTRS)

Niedra, Janis M.

2006-01-01

Room temperature measurements of the turn-off time (t(sub q)) are reported for several packaged, npnp developmental power thyristors based on 4H-type SiC and rated 400 V, 2 A. Turn-off is effected by a 50 V pulse of applied reverse voltage, from a state of a steady 1 A forward current. Plots of t(sub q) against the ramp rate (dV(sub AK)/dt) of reapplied forward voltage are presented for preset values of limiting anode-to-cathode voltage (V(sub AK,max)). The lowest t(sub q) measured was about 180 ns. A rapid rise of these t(sub q) curves was observed for values of V(sub AK,max) that are only about a fifth of the rated voltage, whereas comparative t(sub q) plots for a commercial, fast turn-off, Si-based thyristor at a proportionately reduced V(sub AK,max) showed no such behavior. Hence these SiC thyristors may have problems arising from material defects or surface passivation. The influence the R-C-D gate bypass circuit that was used is briefly discussed.

The SAMI Galaxy Survey: spatially resolving the main sequence of star formation

NASA Astrophysics Data System (ADS)

Medling, Anne M.; Cortese, Luca; Croom, Scott M.; Green, Andrew W.; Groves, Brent; Hampton, Elise; Ho, I.-Ting; Davies, Luke J. M.; Kewley, Lisa J.; Moffett, Amanda J.; Schaefer, Adam L.; Taylor, Edward; Zafar, Tayyaba; Bekki, Kenji; Bland-Hawthorn, Joss; Bloom, Jessica V.; Brough, Sarah; Bryant, Julia J.; Catinella, Barbara; Cecil, Gerald; Colless, Matthew; Couch, Warrick J.; Drinkwater, Michael J.; Driver, Simon P.; Federrath, Christoph; Foster, Caroline; Goldstein, Gregory; Goodwin, Michael; Hopkins, Andrew; Lawrence, J. S.; Leslie, Sarah K.; Lewis, Geraint F.; Lorente, Nuria P. F.; Owers, Matt S.; McDermid, Richard; Richards, Samuel N.; Sharp, Robert; Scott, Nicholas; Sweet, Sarah M.; Taranu, Dan S.; Tescari, Edoardo; Tonini, Chiara; van de Sande, Jesse; Walcher, C. Jakob; Wright, Angus

2018-04-01

We present the ˜800 star formation rate maps for the Sydney-AAO Multi-object Integral field spectrograph (SAMI) Galaxy Survey based on H α emission maps, corrected for dust attenuation via the Balmer decrement, that are included in the SAMI Public Data Release 1. We mask out spaxels contaminated by non-stellar emission using the [O III]/H β, [N II]/H α, [S II]/H α, and [O I]/H α line ratios. Using these maps, we examine the global and resolved star-forming main sequences of SAMI galaxies as a function of morphology, environmental density, and stellar mass. Galaxies further below the star-forming main sequence are more likely to have flatter star formation profiles. Early-type galaxies split into two populations with similar stellar masses and central stellar mass surface densities. The main-sequence population has centrally concentrated star formation similar to late-type galaxies, while galaxies >3σ below the main sequence show significantly reduced star formation most strikingly in the nuclear regions. The split populations support a two-step quenching mechanism, wherein halo mass first cuts off the gas supply and remaining gas continues to form stars until the local stellar mass surface density can stabilize the reduced remaining fuel against further star formation. Across all morphologies, galaxies in denser environments show a decreased specific star formation rate from the outside in, supporting an environmental cause for quenching, such as ram-pressure stripping or galaxy interactions.

Research on the magnetorheological finishing of large aperture off-axis aspheric optical surfaces for zinc sulfide

NASA Astrophysics Data System (ADS)

Zhang, Yunfei; Huang, Wen; Zheng, Yongcheng; Ji, Fang; Xu, Min; Duan, Zhixin; Luo, Qing; Liu, Qian; Xiao, Hong

2016-03-01

Zinc sulfide is a kind of typical infrared optical material, commonly produced using single point diamond turning (SPDT). SPDT can efficiently produce zinc sulfide aspheric surfaces with micro-roughness and acceptable figure error. However the tool marks left by the diamond turning process cause high micro-roughness that degrades the optical performance when used in the visible region of the spectrum. Magnetorheological finishing (MRF) is a deterministic, sub-aperture polishing technology that is very helpful in improving both surface micro-roughness and surface figure.This paper mainly investigates the MRF technology of large aperture off-axis aspheric optical surfaces for zinc sulfide. The topological structure and coordinate transformation of a MRF machine tool PKC1200Q2 are analyzed and its kinematics is calculated, then the post-processing algorithm model of MRF for an optical lens is established. By taking the post-processing of off-axis aspheric surfacefor example, a post-processing algorithm that can be used for a raster tool path is deduced and the errors produced by the approximate treatment are analyzed. A polishing algorithm of trajectory planning and dwell time based on matrix equation and optimization theory is presented in this paper. Adopting this algorithm an experiment is performed to machining a large-aperture off-axis aspheric surface on the MRF machine developed by ourselves. After several times' polishing, the figure accuracy PV is proved from 3.3λ to 2.0λ and RMS from 0.451λ to 0.327λ. This algorithm is used to polish the other shapes including spheres, aspheres and prisms.

In silico identification of novel ligands for G-quadruplex in the c- MYC promoter

NASA Astrophysics Data System (ADS)

Kang, Hyun-Jin; Park, Hyun-Ju

2015-04-01

G-quadruplex DNA formed in NHEIII1 region of oncogene promoter inhibits transcription of the genes. In this study, virtual screening combining pharmacophore-based search and structure-based docking screening was conducted to discover ligands binding to G-quadruplex in promoter region of c- MYC. Several hit ligands showed the selective PCR-arresting effects for oligonucleotide containing c- MYC G-quadruplex forming sequence. Among them, three hits selectively inhibited cell proliferation and decreased c- MYC mRNA level in Ramos cells, where NHEIII1 is included in translocated c- MYC gene for overexpression. Promoter assay using two kinds of constructs with wild-type and mutant sequences showed that interaction of these ligands with the G-quadruplex resulted in turning-off of the reporter gene. In conclusion, combined virtual screening methods were successfully used for discovery of selective c- MYC promoter G-quadruplex binders with anticancer activity.

DNA sequence selectivity of hairpin polyamide turn units

PubMed Central

Farkas, Michelle E.; Li, Benjamin C.; Dose, Christian; Dervan, Peter B.

2011-01-01

A class of hairpin polyamides linked by 3,4-diaminobutyric acid, resulting in a β-amine residue at the turn unit, showed improved binding affinities relative to their α-amino-γ-turn analogs for particular sequences. We incorporated β-amino-γ-turns in six-ring polyamides and determined whether there are any sequence preferences under the turn unit by quantitative footprinting titrations. Although there was an energetic penalty for G·C and C·G base pairs, we found little preference for T·A over A·T at the β-amino-γ-turn position. Fluorine and hydroxyl substituted α-amino-γ-turns were synthesized for comparison. Their binding affinities and specificities in the context of six-ring polyamides demonstrated overall diminished affinity and no additional specificity at the turn position. We anticipate that this study will be a baseline for further investigation of the turn subunit as a recognition element for the DNA minor groove. PMID:19349175

Extended turn construction and test question sequences in the conversations of three speakers with agrammatic aphasia

PubMed Central

Beckley, Firle; Best, Wendy; Johnson, Fiona; Edwards, Susan; Maxim, Jane

2013-01-01

The application of Conversation Analysis (CA) to the investigation of agrammatic aphasia reveals that utterances produced by speakers with agrammatism engaged in everyday conversation differ significantly from utterances produced in response to decontextualised assessment and therapy tasks. Early studies have demonstrated that speakers with agrammatism construct turns from sequences of nouns, adjectives, discourse markers and conjunctions, packaged by a distinct pattern of prosody. This article presents examples of turn construction methods deployed by three people with agrammatism as they take an extended turn, in order to recount a past event, initiate a discussion or have a disagreement. This is followed by examples of sequences occurring in the talk of two of these speakers that result in different, and more limited, turn construction opportunities, namely “test” questions asked in order to initiate a new topic of talk, despite the conversation partner knowing the answer. The contrast between extended turns and test question sequences illustrates the effect of interactional context on aphasic turn construction practices, and the potential of less than optimal sequences to mask turn construction skills. It is suggested that the interactional motivation for test question sequences in these data are to invite people with aphasia to contribute to conversation, rather than to practise saying words in an attempt to improve language skills. The idea that test question sequences may have their origins in early attempts to deal with acute aphasia, and the potential for conversation partnerships to become “stuck” in such interactional patterns after they may have outlived their usefulness, are discussed with a view to clinical implications. PMID:23848370

Molecular dynamics study of the phosphorylation effect on the conformational states of the C-terminal domain of RNA polymerase II.

PubMed

Yonezawa, Yasushige

2014-05-01

The carboxyl-terminal domain (CTD) of RNA polymerase II in eukaryotes regulates mRNA processing processes by recruiting various regulation factors. A main function of the CTD relies on the heptad consensus sequence (YSPTSPS). The CTD dynamically changes its conformational state to recognize and bind different regulation factors. The dynamical conformation changes are caused by modifications, mainly phosphorylation and dephosphorylation, to the serine residues. In this study, we investigate the conformational states of the unit consensus CTD peptide with various phosphorylation patterns of the serine residues by extended ensemble simulations. The results show that the CTD without phosphorylation has a flexible disordered structure distributed between twisted and extended states, but phosphorylation tends to reduce the conformational space. It was found that phosphorylation induces a β-turn around the phosphorylated serine residue and the cis conformation of the proline residue significantly inhibits the β-turn formation. The β-turn should contribute to specific CTD binding of the different regulation factors by changing the conformation propensity combined with induced fit.

Schwarzschild, Martin (1912-97)

NASA Astrophysics Data System (ADS)

Murdin, P.

2000-11-01

Astrophysicist, born in Potsdam, Germany, the son of KARL SCHWARZSCHILD, left Germany, became professor at Princeton University. Working with John von Neumann, Schwarzschild used the powers of the newly developed electronic digital computers to work on the theory of stellar structure and evolution. He uncovered phenomena in red giant stars, including how they evolve off the main sequence in the H...

Sequential power-up circuit

DOEpatents

Kronberg, J.W.

1992-06-02

A sequential power-up circuit for starting several electrical load elements in series to avoid excessive current surge, comprising a voltage ramp generator and a set of voltage comparators, each comparator having a different reference voltage and interfacing with a switch that is capable of turning on one of the load elements. As the voltage rises, it passes the reference voltages one at a time and causes the switch corresponding to that voltage to turn on its load element. The ramp is turned on and off by a single switch or by a logic-level electrical signal. The ramp rate for turning on the load element is relatively slow and the rate for turning the elements off is relatively fast. Optionally, the duration of each interval of time between the turning on of the load elements is programmable. 2 figs.

NetTurnP – Neural Network Prediction of Beta-turns by Use of Evolutionary Information and Predicted Protein Sequence Features

PubMed Central

Petersen, Bent; Lundegaard, Claus; Petersen, Thomas Nordahl

2010-01-01

β-turns are the most common type of non-repetitive structures, and constitute on average 25% of the amino acids in proteins. The formation of β-turns plays an important role in protein folding, protein stability and molecular recognition processes. In this work we present the neural network method NetTurnP, for prediction of two-class β-turns and prediction of the individual β-turn types, by use of evolutionary information and predicted protein sequence features. It has been evaluated against a commonly used dataset BT426, and achieves a Matthews correlation coefficient of 0.50, which is the highest reported performance on a two-class prediction of β-turn and not-β-turn. Furthermore NetTurnP shows improved performance on some of the specific β-turn types. In the present work, neural network methods have been trained to predict β-turn or not and individual β-turn types from the primary amino acid sequence. The individual β-turn types I, I', II, II', VIII, VIa1, VIa2, VIba and IV have been predicted based on classifications by PROMOTIF, and the two-class prediction of β-turn or not is a superset comprised of all β-turn types. The performance is evaluated using a golden set of non-homologous sequences known as BT426. Our two-class prediction method achieves a performance of: MCC  = 0.50, Qtotal = 82.1%, sensitivity  = 75.6%, PPV  = 68.8% and AUC  = 0.864. We have compared our performance to eleven other prediction methods that obtain Matthews correlation coefficients in the range of 0.17 – 0.47. For the type specific β-turn predictions, only type I and II can be predicted with reasonable Matthews correlation coefficients, where we obtain performance values of 0.36 and 0.31, respectively. Conclusion The NetTurnP method has been implemented as a webserver, which is freely available at http://www.cbs.dtu.dk/services/NetTurnP/. NetTurnP is the only available webserver that allows submission of multiple sequences. PMID:21152409

NetTurnP--neural network prediction of beta-turns by use of evolutionary information and predicted protein sequence features.

PubMed

Petersen, Bent; Lundegaard, Claus; Petersen, Thomas Nordahl

2010-11-30

β-turns are the most common type of non-repetitive structures, and constitute on average 25% of the amino acids in proteins. The formation of β-turns plays an important role in protein folding, protein stability and molecular recognition processes. In this work we present the neural network method NetTurnP, for prediction of two-class β-turns and prediction of the individual β-turn types, by use of evolutionary information and predicted protein sequence features. It has been evaluated against a commonly used dataset BT426, and achieves a Matthews correlation coefficient of 0.50, which is the highest reported performance on a two-class prediction of β-turn and not-β-turn. Furthermore NetTurnP shows improved performance on some of the specific β-turn types. In the present work, neural network methods have been trained to predict β-turn or not and individual β-turn types from the primary amino acid sequence. The individual β-turn types I, I', II, II', VIII, VIa1, VIa2, VIba and IV have been predicted based on classifications by PROMOTIF, and the two-class prediction of β-turn or not is a superset comprised of all β-turn types. The performance is evaluated using a golden set of non-homologous sequences known as BT426. Our two-class prediction method achieves a performance of: MCC=0.50, Qtotal=82.1%, sensitivity=75.6%, PPV=68.8% and AUC=0.864. We have compared our performance to eleven other prediction methods that obtain Matthews correlation coefficients in the range of 0.17-0.47. For the type specific β-turn predictions, only type I and II can be predicted with reasonable Matthews correlation coefficients, where we obtain performance values of 0.36 and 0.31, respectively. The NetTurnP method has been implemented as a webserver, which is freely available at http://www.cbs.dtu.dk/services/NetTurnP/. NetTurnP is the only available webserver that allows submission of multiple sequences.

ω-Turn: a novel β-turn mimic in globular proteins stabilized by main-chain to side-chain C−H···O interaction.

PubMed

Dhar, Jesmita; Chakrabarti, Pinak; Saini, Harpreet; Raghava, Gajendra Pal Singh; Kishore, Raghuvansh

2015-02-01

Mimicry of structural motifs is a common feature in proteins. The 10-membered hydrogen-bonded ring involving the main-chain C − O in a β-turn can be formed using a side-chain carbonyl group leading to Asx-turn. We show that the N − H component of hydrogen bond can be replaced by a C(γ) -H group in the side chain, culminating in a nonconventional C − H···O interaction. Because of its shape this β-turn mimic is designated as ω-turn, which is found to occur ∼ three times per 100 residues. Three residues (i to i + 2) constitute the turn with the C − H···O interaction occurring between the terminal residues, constraining the torsion angles ϕi + 1, ψi + 1, ϕi + 2 and χ'1(i + 2) (using the interacting C(γ) atom). Based on these angles there are two types of ω-turns, each of which can be further divided into two groups. C(β) -branched side-chains, and Met and Gln have high propensities to occur at i + 2; for the last two residues the carbonyl oxygen may participate in an additional interaction involving the S and amino group, respectively. With Cys occupying the i + 1 position, such turns are found in the metal-binding sites. N-linked glycosylation occurs at the consensus pattern Asn-Xaa-Ser/Thr; with Thr at i + 2, the sequence can adopt the secondary structure of a ω-turn, which may be the recognition site for protein modification. Location between two β-strands is the most common occurrence in protein tertiary structure, and being generally exposed ω-turn may constitute the antigenic determinant site. It is a stable scaffold and may be used in protein engineering and peptide design. © 2014 Wiley Periodicals, Inc.

A Logical OR Redundancy within the Asx-Pro-Asx-Gly Type 1 {Beta}-Turn Motif

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lee, Jihun; Dubey, Vikash Kumar; Longo, Lian M.

2008-04-19

Turn secondary structure is essential to the formation of globular protein architecture. Turn structures are, however, much more complex than either {alpha}-helix or {beta}-sheet, and the thermodynamics and folding kinetics are poorly understood. Type I {beta}-turns are the most common type of reverse turn, and they exhibit a statistical consensus sequence of Asx-Pro-Asx-Gly (where Asx is Asp or Asn). A comprehensive series of individual and combined Asx mutations has been constructed within three separate type I 3:5 G1 bulge {beta}-turns in human fibroblast growth factor-1, and their effects on structure, stability, and folding have been determined. The results show amore » fundamental logical OR relationship between the Asx residues in the motif, involving H-bond interactions with main-chain amides within the turn. These interactions can be modulated by additional interactions with residues adjacent to the turn at positions i + 4 and i + 6. The results show that the Asx residues in the turn motif make a substantial contribution to the overall stability of the protein, and the Asx logical OR relationship defines a redundant system that can compensate for deleterious point mutations. The results also show that the stability of the turn is unlikely to be the prime determinant of formation of turn structure in the folding transition state.« less

Redesigning the type II' β-turn in green fluorescent protein to type I': implications for folding kinetics and stability.

PubMed

Madan, Bharat; Sokalingam, Sriram; Raghunathan, Govindan; Lee, Sun-Gu

2014-10-01

Both Type I' and Type II' β-turns have the same sense of the β-turn twist that is compatible with the β-sheet twist. They occur predominantly in two residue β-hairpins, but the occurrence of Type I' β-turns is two times higher than Type II' β-turns. This suggests that Type I' β-turns may be more stable than Type II' β-turns, and Type I' β-turn sequence and structure can be more favorable for protein folding than Type II' β-turns. Here, we redesigned the native Type II' β-turn in GFP to Type I' β-turn, and investigated its effect on protein folding and stability. The Type I' β-turns were designed based on the statistical analysis of residues in natural Type I' β-turns. The substitution of the native "GD" sequence of i+1 and i+2 residues with Type I' preferred "(N/D)G" sequence motif increased the folding rate by 50% and slightly improved the thermodynamic stability. Despite the enhancement of in vitro refolding kinetics and stability of the redesigned mutants, they showed poor soluble expression level compared to wild type. To overcome this problem, i and i + 3 residues of the designed Type I' β-turn were further engineered. The mutation of Thr to Lys at i + 3 could restore the in vivo soluble expression of the Type I' mutant. This study indicates that Type II' β-turns in natural β-hairpins can be further optimized by converting the sequence to Type I'. © 2014 Wiley Periodicals, Inc.

An Integrated Power-Efficient Active Rectifier With Offset-Controlled High Speed Comparators for Inductively Powered Applications

PubMed Central

Lee, Hyung-Min; Ghovanloo, Maysam

2011-01-01

We present an active full-wave rectifier with offset-controlled high speed comparators in standard CMOS that provides high power conversion efficiency (PCE) in high frequency (HF) range for inductively powered devices. This rectifier provides much lower dropout voltage and far better PCE compared to the passive on-chip or off-chip rectifiers. The built-in offset-control functions in the comparators compensate for both turn-on and turn-off delays in the main rectifying switches, thus maximizing the forward current delivered to the load and minimizing the back current to improve the PCE. We have fabricated this active rectifier in a 0.5-μm 3M2P standard CMOS process, occupying 0.18 mm2 of chip area. With 3.8 V peak ac input at 13.56 MHz, the rectifier provides 3.12 V dc output to a 500 Ω load, resulting in the PCE of 80.2%, which is the highest measured at this frequency. In addition, overvoltage protection (OVP) as safety measure and built-in back telemetry capabilities have been incorporated in our design using detuning and load shift keying (LSK) techniques, respectively, and tested. PMID:22174666

Photoluminescent carbon dots based on a rare 3D inorganic-organic hybrid cadmium borate crystal.

PubMed

Zhou, Kang; Zhang, Wen-Jin; Luo, Yuan-Zhang; Pan, Chun-Yang

2018-05-17

A 3D inorganic-organic hybridized skeleton cadmium borate [Cden][B5O8(OH)] (1) (en = ethylenediamine) has been solvothermally synthesized. By calcining it, specific shape carbon dots (C-dots) with abundant free radicals were observed. In addition, C-dots in the ethanol phase exhibited variable photoluminescence and showed rare turn on or off effects to Cr3+ ions and CdSe/ZnS quantum dots, but only a turn on effect to Cs+ ions and a turn off effect to CsPbBr3 quantum dots.

Geophysics: Timing of the Martian dynamo

NASA Astrophysics Data System (ADS)

Schubert, G.; Russell, C. T.; Moore, W. B.

2000-12-01

On Mars, the strong magnetization in the highland crust of the southern hemisphere and the absence of magnetic anomalies at the Hellas and Argyre impact basins have been taken as signs that the core dynamo that once drove the planet's magnetic field turned off more than 4 billion years (Gyr) ago. Here, we argue instead that the Martian dynamo turned on less than 4 Gyr ago and turned off at an unknown time since then. High spatial resolution magnetometry in both Martian hemispheres is needed to reveal the true history of the Martian dynamo.

Getting in Taped, Part I and Part II

ERIC Educational Resources Information Center

Cundy, H. M.; Higgins, J.

1971-01-01

This article is in two parts: discussion of mathematical concepts involved in converting the reading from the tape-recorder counter which counts the turns of the run-off spool to that from the counter which counts turns of the take-up spool; calculating the length of tape run off when given the reading from the tape-recorder counter of the run-off…

Gait bradykinesia in Parkinson's disease: a change in the motor program which controls the synergy of gait.

PubMed

Warabi, Tateo; Furuyama, Hiroyasu; Sugai, Eri; Kato, Masamichi; Yanagisawa, Nobuo

2018-01-01

This study examined how gait bradykinesia is changed by the motor programming in Parkinson's disease. Thirty-five idiopathic Parkinson's disease patients and nine age-matched healthy subjects participated in this study. After the patients fixated on a visual-fixation target (conditioning-stimulus), the voluntary-gait was triggered by a visual on-stimulus. While the subject walked on a level floor, soleus, tibialis anterior EMG latencies, and the y-axis-vector of the sole-floor reaction force were examined. Three paradigms were used to distinguish between the off-/on-latencies. The gap-task: the visual-fixation target was turned off; 200 ms before the on-stimulus was engaged (resulting in a 200 ms-gap). EMG latency was not influenced by the visual-fixation target. The overlap-task: the on-stimulus was turned on during the visual-fixation target presentation (200 ms-overlap). The no-gap-task: the fixation target was turned off and the on-stimulus was turned on simultaneously. The onset of EMG pause following the tonic soleus EMG was defined as the off-latency of posture (termination). The onset of the tibialis anterior EMG burst was defined as the on-latency of gait (initiation). In the gap-task, the on-latency was unchanged in all of the subjects. In Parkinson's disease, the visual-fixation target prolonged both the off-/on-latencies in the overlap-task. In all tasks, the off-latency was prolonged and the off-/on-latencies were unsynchronized, which changed the synergic movement to a slow, short-step-gait. The synergy of gait was regulated by two independent sensory-motor programs of the off- and on-latency levels. In Parkinson's disease, the delayed gait initiation was due to the difficulty in terminating the sensory-motor program which controls the subject's fixation. The dynamic gait bradykinesia was involved in the difficulty (long off-latency) in terminating the motor program of the prior posture/movement.

Characterization of beta-turn and Asx-turns mimicry in a model peptide: stabilization via C--H . . . O interaction.

PubMed

Thakur, A K; Kishore, R

2006-04-15

The chemical synthesis and single-crystal X-ray diffraction analysis of a model peptide, Boc-Thr-Thr-NH2 (1) comprised of proteinogenic residues bearing an amphiphilic Cbeta -stereogenic center, has been described. Interestingly, the analysis of its molecular structure revealed the existence of a distinct conformation that mimics a typical beta-turn and Asx-turns, i.e., the two Thr residues occupy the left- and right-corner positions. The main-chain torsion angles of the N- and C-terminal residues i.e., semiextended: phi = -68.9 degrees , psi = 128.6 degrees ; semifolded: phi = -138.1 degrees , psi = 2.5 degrees conformations, respectively, in conjunction with a gauche- disposition of the obligatory C-terminus Thr CgammaH3 group, characterize the occurrence of the newly described beta-turn- and Asx-turns-like topology. The preferred molecular structure is suggested to be stabilized by an effective nonconventional main-chain to side-chain Ci=O . . . H--Cgamma(i+2)-type intraturn hydrogen bond. Noteworthy, the observed topology of the resulting 10-membered hydrogen-bonded ring is essentially similar to the one perceived for a classical beta-turn and the Asx-turns, stabilized by a conventional intraturn hydrogen bond. Considering the signs as well as magnitudes of the backbone torsion angles and the orientation of the central peptide bond, the overall mimicked topology resembles the type II beta-turn or type II Asx-turns. An analysis of Xaa-Thr sequences in high-resolution X-ray elucidated protein structures revealed the novel topology prevalence in functional proteins (unpublished). In view of indubitable structural as well as functional importance of nonconventional interactions in bioorganic and biomacromolecules, we intend to highlight the participation of Thr CgammaH in the creation of a short-range C=O . . . H--Cgamma -type interaction in peptides and proteins. Copyright 2006 Wiley Periodicals, Inc.

Role of local sequence in the folding of cellular retinoic abinding protein I: structural propensities of reverse turns.

PubMed

Rotondi, Kenneth S; Gierasch, Lila M

2003-07-08

The experiments described here explore the role of local sequence in the folding of cellular retinoic acid binding protein I (CRABP I). This is a 136-residue, 10-stranded, antiparallel beta-barrel protein with seven beta-hairpins and is a member of the intracellular lipid binding protein (iLBP) family. The relative roles of local and global sequence information in governing the folding of this class of proteins are not well-understood. In question is whether the beta-turns are locally defined by short-range interactions within their sequences, and are thus able to play an active role in reducing the conformational space available to the folding chain, or whether the turns are passive, relying upon global forces to form. Short (six- and seven-residue) peptides corresponding to the seven CRABP I turns were analyzed by circular dichroism and NMR for their tendencies to take up the conformations they adopt in the context of the native protein. The results indicate that two of the peptides, encompassing turns III and IV in CRABP I, have a strong intrinsic bias to form native turns. Intriguingly, these turns are on linked hairpins in CRABP I and represent the best-conserved turns in the iLBP family. These results suggest that local sequence may play an important role in narrowing the conformational ensemble of CRABP I during folding.

Dual control and prevention of the turn-off phenomenon in a class of mimo systems

NASA Technical Reports Server (NTRS)

Mookerjee, P.; Bar-Shalom, Y.; Molusis, J. A.

1985-01-01

A recently developed methodology of adaptive dual control based upon sensitivity functions is applied here to a multivariable input-output model. The plant has constant but unknown parameters. It represents a simplified linear version of the relationship between the vibration output and the higher harmonic control input for a helicopter. The cautious and the new dual controller are examined. In many instances, the cautious controller is seen to turn off. The new dual controller modifies the cautious control design by numerator and denominator correction terms which depend upon the sensitivity functions of the expected future cost and avoids the turn-off and burst phenomena. Monte Carlo simulations and statistical tests of significance indicate the superiority of the dual controller over the cautious and the heuristic certainity equivalence controllers.

Radio Emission from Red-Giant Hot Jupiters

NASA Technical Reports Server (NTRS)

Fujii, Yuka; Spiegel, David S.; Mroczkowski, Tony; Nordhaus, Jason; Zimmerman, Neil T.; Parsons, Aaron R.; Mirbabayi, Mehrdad; Madhusudhan, Nikku

2016-01-01

When planet-hosting stars evolve off the main sequence and go through the red-giant branch, the stars become orders of magnitudes more luminous and, at the same time, lose mass at much higher rates than their main sequence counterparts. Accordingly, if planetary companions exist around these stars at orbital distances of a few au, they will be heated up to the level of canonical hot Jupiters and also be subjected to a dense stellar wind. Given that magnetized planets interacting with stellar winds emit radio waves, such "Red-Giant Hot Jupiters" (RGHJs) may also be candidate radio emitters. We estimate the spectral auroral radio intensity of RGHJs based on the empirical relation with the stellar wind as well as a proposed scaling for planetary magnetic fields. RGHJs might be intrinsically as bright as or brighter than canonical hot Jupiters and about 100 times brighter than equivalent objects around main-sequence stars. We examine the capabilities of low-frequency radio observatories to detect this emission and find that the signal from an RGHJ may be detectable at distances up to a few hundred parsecs with the Square Kilometer Array.

Alignment Tool For Inertia Welding

NASA Technical Reports Server (NTRS)

Snyder, Gary L.

1991-01-01

Compact, easy-to-use tool aligns drive bar of inertia welder over hole in stub. Ensures drive bar concentric to hole within 0.002 in. (0.051 mm.). Holds two batteries and light bulb. Electrical circuit completed, providing current to bulb when pin in contact with post. When pin centered in post hole, it does not touch post, and lamp turns off. Built for use in making repair welds on liquid-oxygen-injector posts in Space Shuttle main engine. Version having suitably modified dimensions used to facilitate alignment in other forests of post.

Relative Suffix Trees.

PubMed

Farruggia, Andrea; Gagie, Travis; Navarro, Gonzalo; Puglisi, Simon J; Sirén, Jouni

2018-05-01

Suffix trees are one of the most versatile data structures in stringology, with many applications in bioinformatics. Their main drawback is their size, which can be tens of times larger than the input sequence. Much effort has been put into reducing the space usage, leading ultimately to compressed suffix trees. These compressed data structures can efficiently simulate the suffix tree, while using space proportional to a compressed representation of the sequence. In this work, we take a new approach to compressed suffix trees for repetitive sequence collections, such as collections of individual genomes. We compress the suffix trees of individual sequences relative to the suffix tree of a reference sequence. These relative data structures provide competitive time/space trade-offs, being almost as small as the smallest compressed suffix trees for repetitive collections, and competitive in time with the largest and fastest compressed suffix trees.

Relative Suffix Trees

PubMed Central

Farruggia, Andrea; Gagie, Travis; Navarro, Gonzalo; Puglisi, Simon J; Sirén, Jouni

2018-01-01

Abstract Suffix trees are one of the most versatile data structures in stringology, with many applications in bioinformatics. Their main drawback is their size, which can be tens of times larger than the input sequence. Much effort has been put into reducing the space usage, leading ultimately to compressed suffix trees. These compressed data structures can efficiently simulate the suffix tree, while using space proportional to a compressed representation of the sequence. In this work, we take a new approach to compressed suffix trees for repetitive sequence collections, such as collections of individual genomes. We compress the suffix trees of individual sequences relative to the suffix tree of a reference sequence. These relative data structures provide competitive time/space trade-offs, being almost as small as the smallest compressed suffix trees for repetitive collections, and competitive in time with the largest and fastest compressed suffix trees. PMID:29795706

Analysis of the lateral push-off in the freestyle flip turn.

PubMed

Araujo, Luciana; Pereira, Suzana; Gatti, Roberta; Freitas, Elinai; Jacomel, Gabriel; Roesler, Helio; Villas-Boas, Joao

2010-09-01

The aim of this study was to examine the contact phase during the lateral push-off in the turn of front crawl swimming to determine which biomechanical variables (maximum normalized peak force, contact time, impulse, angle of knee flexion, and total turn time within 15 m) contribute to the performance of this turn technique. Thirty-four swimmers of state, national, and international competitive standard participated in the study. For data collection, the following equipment was used: an underwater force platform, a 30-Hz VHS video camera, and a MiniDv digital camera within an underwater box. Data are expressed as descriptive statistics. Inferential analyses were performed using Pearson's correlation and multiple linear regressions. All variables studied had a significant relationship with turn performance. We conclude that a turn executed with a knee flexion angle of between 100° and 120° provides optimum peak forces to generate impulses that allow the swimmer to lose less time in the turn without the need for an excessive force application and with less energy lost.

Huge Inverse Magnetization Generated by Faraday Induction in Nano-Sized Au@Ni Core@Shell Nanoparticles.

PubMed

Kuo, Chen-Chen; Li, Chi-Yen; Lee, Chi-Hung; Li, Hsiao-Chi; Li, Wen-Hsien

2015-08-25

We report on the design and observation of huge inverse magnetizations pointing in the direction opposite to the applied magnetic field, induced in nano-sized amorphous Ni shells deposited on crystalline Au nanoparticles by turning the applied magnetic field off. The magnitude of the induced inverse magnetization is very sensitive to the field reduction rate as well as to the thermal and field processes before turning the magnetic field off, and can be as high as 54% of the magnetization prior to cutting off the applied magnetic field. Memory effect of the induced inverse magnetization is clearly revealed in the relaxation measurements. The relaxation of the inverse magnetization can be described by an exponential decay profile, with a critical exponent that can be effectively tuned by the wait time right after reaching the designated temperature and before the applied magnetic field is turned off. The key to these effects is to have the induced eddy current running beneath the amorphous Ni shells through Faraday induction.

Optimized protocols for cardiac magnetic resonance imaging in patients with thoracic metallic implants.

PubMed

Olivieri, Laura J; Cross, Russell R; O'Brien, Kendall E; Ratnayaka, Kanishka; Hansen, Michael S

2015-09-01

Cardiac magnetic resonance (MR) imaging is a valuable tool in congenital heart disease; however patients frequently have metal devices in the chest from the treatment of their disease that complicate imaging. Methods are needed to improve imaging around metal implants near the heart. Basic sequence parameter manipulations have the potential to minimize artifact while limiting effects on image resolution and quality. Our objective was to design cine and static cardiac imaging sequences to minimize metal artifact while maintaining image quality. Using systematic variation of standard imaging parameters on a fluid-filled phantom containing commonly used metal cardiac devices, we developed optimized sequences for steady-state free precession (SSFP), gradient recalled echo (GRE) cine imaging, and turbo spin-echo (TSE) black-blood imaging. We imaged 17 consecutive patients undergoing routine cardiac MR with 25 metal implants of various origins using both standard and optimized imaging protocols for a given slice position. We rated images for quality and metal artifact size by measuring metal artifact in two orthogonal planes within the image. All metal artifacts were reduced with optimized imaging. The average metal artifact reduction for the optimized SSFP cine was 1.5+/-1.8 mm, and for the optimized GRE cine the reduction was 4.6+/-4.5 mm (P < 0.05). Quality ratings favored the optimized GRE cine. Similarly, the average metal artifact reduction for the optimized TSE images was 1.6+/-1.7 mm (P < 0.05), and quality ratings favored the optimized TSE imaging. Imaging sequences tailored to minimize metal artifact are easily created by modifying basic sequence parameters, and images are superior to standard imaging sequences in both quality and artifact size. Specifically, for optimized cine imaging a GRE sequence should be used with settings that favor short echo time, i.e. flow compensation off, weak asymmetrical echo and a relatively high receiver bandwidth. For static black-blood imaging, a TSE sequence should be used with fat saturation turned off and high receiver bandwidth.

Dancing with Mobile Devices: The LAIT Application System in Performance and Educational Settings

ERIC Educational Resources Information Center

Toenjes, John; Beck, Ken; Reimer, M. Anthony; Mott, Erica

2016-01-01

At most any performance today, you will be notified to turn off your cell phone. The smartphone has become such an integral tool in our daily lives that turning it off is tantamount to severing our connection to our community and challenging the way we view and negotiate the world. Many audience members, particularly young ones, will be looking at…

Do Rural and Regional Students in Queensland Experience an ICT "Turn-Off" in the Early High School Years?

ERIC Educational Resources Information Center

Courtney, Lyn; Anderson, Neil

2010-01-01

Students learning in regional, rural and remote locations in Queensland are currently experiencing a "turn-off" in relation to school-based ICT in the first three years of high school. At the same time, students are experiencing increasing levels of interest and motivation from their use of ICT at home. Given the importance of ICT as an…

The siRNA Non-seed Region and Its Target Sequences Are Auxiliary Determinants of Off-Target Effects.

PubMed

Kamola, Piotr J; Nakano, Yuko; Takahashi, Tomoko; Wilson, Paul A; Ui-Tei, Kumiko

2015-12-01

RNA interference (RNAi) is a powerful tool for post-transcriptional gene silencing. However, the siRNA guide strand may bind unintended off-target transcripts via partial sequence complementarity by a mechanism closely mirroring micro RNA (miRNA) silencing. To better understand these off-target effects, we investigated the correlation between sequence features within various subsections of siRNA guide strands, and its corresponding target sequences, with off-target activities. Our results confirm previous reports that strength of base-pairing in the siRNA seed region is the primary factor determining the efficiency of off-target silencing. However, the degree of downregulation of off-target transcripts with shared seed sequence is not necessarily similar, suggesting that there are additional auxiliary factors that influence the silencing potential. Here, we demonstrate that both the melting temperature (Tm) in a subsection of siRNA non-seed region, and the GC contents of its corresponding target sequences, are negatively correlated with the efficiency of off-target effect. Analysis of experimentally validated miRNA targets demonstrated a similar trend, indicating a putative conserved mechanistic feature of seed region-dependent targeting mechanism. These observations may prove useful as parameters for off-target prediction algorithms and improve siRNA 'specificity' design rules.

RNA Tertiary Interactions in a Riboswitch Stabilize the Structure of a Kink Turn

PubMed Central

Schroeder, Kersten T.; Daldrop, Peter; Lilley, David M.J.

2011-01-01

Summary The kink turn is a widespread RNA motif that introduces an acute kink into the axis of duplex RNA, typically comprising a bulge followed by a G⋅A and A⋅G pairs. The kinked conformation is stabilized by metal ions, or the binding of proteins including L7Ae. We now demonstrate a third mechanism for the stabilization of k-turn structure, involving tertiary interactions within a larger RNA structure. The SAM-I riboswitch contains an essential standard k-turn sequence that kinks a helix so that its terminal loop can make a long-range interaction. We find that some sequence variations in the k-turn within the riboswitch do not prevent SAM binding, despite preventing the folding of the k-turn in isolation. Furthermore, two crystal structures show that the sequence-variant k-turns are conventionally folded within the riboswitch. This study shows that the folded structure of the k-turn can be stabilized by tertiary interactions within a larger RNA structure. PMID:21893284

Measures of Attitudes Toward and Communication about Condom Use: Their Relationships With Sexual Risk Behavior Among Young Black MSM

PubMed Central

Crosby, Richard A.; Graham, Cynthia A.; Yarber, William L.; Sanders, Stephanie A.; Milhausen, Robin R.; Mena, Leandro

2015-01-01

Objective To construct and test measures of psychosocial mediators that could be used in intervention studies seeking to promote safer sex behavior among young Black men who have sex with men (YBMSM). Methods YBMSM (N=400), ages 18–29 years, were recruited from an STI clinic, in the Southern U.S. All men had engaged in penile-anal sex with a male as a “top” in the past 6 months. Men completed an audio-computer assisted self-interview and provided specimens used for NAAT testing to detect Chlamydia and gonorrhea. Four measures were constructed and tested for criterion validity (Safer Sex Communication, Condom Turn-Offs, Condom Pleasure Scale, and a single item assessing frequency of condom use discussions before sexual arousal). Results With the exception of Safer Sex Communication, all of the measures showed criterion validity for both unprotected anal insertive, and unprotected anal receptive sex. With the exception of the Condom Turn-Offs, the three other measures were supported by criterion validity for oral sex. Both the Condom Turn-Offs and Condom Pleasure Scale were significantly related to whether or not men reported multiple partners as a “top” but only the Condom Pleasure Scale was associated with reports of multiple partners as a “bottom.” Only the Condom Turn-Offs Scale was positively associated with having been diagnosed with either Chlamydia or gonorrhea. Conclusion Findings provide three brief scales and a single item that can be used in intervention studies targeting YBMSM. Perceptions about condoms a turn off and about condoms enhancing pleasure showed strong association with sexual risk behaviors. PMID:26766525

Accuracy of Voltage Signal Measurement During Radiofrequency Delivery Through the SMARTTOUCH Catheter.

PubMed

Safavi-Naeini, Payam; Zafar-Awan, Dreema; Zhu, Hongjian; Zablah, Gerardo; Ganapathy, Anand V; Rasekh, Abdi; Saeed, Mohammad; Razavi, Joanna Esther Molina; Razavi, Mehdi

2017-01-01

Current methods for measuring voltage during radiofrequency (RF) ablation (RFA) necessitate turning off the ablation catheter. If voltage could be accurately read without signal attenuation during RFA, turning off the catheter would be unnecessary, allowing continuous ablation. We evaluated the accuracy of the Thermocool SMARTTOUCH catheter for measuring voltage while RF traverses the catheter. We studied 26 patients undergoing RFA for arrhythmias. A 7.5F SMARTTOUCH catheter was used for sensing voltage and performing RFA. Data were collected from the Carto-3 3-dimensional mapping system. Voltages were measured during ablation (RF-ON) and immediately before or after ablation (RF-OFF). In evaluating the accuracy of RF-ON measurements, we utilized the RF-OFF measure as the gold standard. We measured 465 voltage signals. The median values were 0.2900 and 0.3100 for RF-ON and RF-OFF, respectively. Wilcoxon signed rank testing showed no significant difference in these values (P = 0.608). The intraclass correlation coefficient (ICC) was 0.96, indicating that voltage measurements were similarly accurate during RF-OFF versus RF-ON. Five patients had baseline atrial fibrillation (AF), for whom 82 ablation points were measured; 383 additional ablation points were measured for the remaining patients. The voltages measured during RF-ON versus RF-OFF were similar in the presence of AF (P = 0.800) versus non-AF rhythm (P = 0.456) (ICC, 0.96 for both). Voltage signal measurement was similarly accurate during RF-ON versus RF-OFF independent of baseline rhythm. Physicians should consider not turning off the SMARTTOUCH ablation catheter when measuring voltage during RFA. © 2016 Wiley Periodicals, Inc.

Turn stability in beta-hairpin peptides: Investigation of peptides containing 3:5 type I G1 bulge turns.

PubMed

Blandl, Tamas; Cochran, Andrea G; Skelton, Nicholas J

2003-02-01

The turn-forming ability of a series of three-residue sequences was investigated by substituting them into a well-characterized beta-hairpin peptide. The starting scaffold, bhpW, is a disulfide-cyclized 10-residue peptide that folds into a stable beta-hairpin with two antiparallel strands connected by a two-residue reverse turn. Substitution of the central two residues with the three-residue test sequences leads to less stable hairpins, as judged by thiol-disulfide equilibrium measurements. However, analysis of NMR parameters indicated that each molecule retains a significant folded population, and that the type of turn adopted by the three-residue sequence is the same in all cases. The solution structure of a selected peptide with a PDG turn contained an antiparallel beta-hairpin with a 3:5 type I + G1 bulge turn. Analysis of the energetic contributions of individual turn residues in the series of peptides indicates that substitution effects have significant context dependence, limiting the predictive power of individual amino acid propensities for turn formation. The most stable and least stable sequences were also substituted into a more stable disulfide-cyclized scaffold and a linear beta-hairpin scaffold. The relative stabilities remained the same, suggesting that experimental measurements in the bhpW context are a useful way to evaluate turn stability for use in protein design projects. Moreover, these scaffolds are capable of displaying a diverse set of turns, which can be exploited for the mimicry of protein loops or for generating libraries of reverse turns.

Off-Label Uses of Anti-TNF Therapy in Three Frequent Disorders: Behçet's Disease, Sarcoidosis, and Noninfectious Uveitis

PubMed Central

Sánchez-Cano, Daniel; Callejas-Rubio, José Luis; Ruiz-Villaverde, Ricardo; Ríos-Fernández, Raquel; Ortego-Centeno, Norberto

2013-01-01

Tumoral necrosis factor α plays a central role in both the inflammatory response and that of the immune system. Thus, its blockade with the so-called anti-TNF agents (infliximab, etanercept, adalimumab, certolizumab pegol, and golimumab) has turned into the most important tool in the management of a variety of disorders, such as rheumatoid arthritis, spondyloarthropatties, inflammatory bowel disease, and psoriasis. Nonetheless, theoretically, some other autoimmune disorders may benefit from these agents. Our aim is to review these off-label uses of anti-TNF blockers in three common conditions: Behçet's disease, sarcoidosis, and noninfectious uveitis. Due to the insufficient number of adequate clinical trials and consequently to their lower prevalence compared to other immune disorders, this review is mainly based on case reports and case series. PMID:23983404

Cutting planes for the multistage stochastic unit commitment problem

DOE PAGES

Jiang, Ruiwei; Guan, Yongpei; Watson, Jean -Paul

2016-04-20

As renewable energy penetration rates continue to increase in power systems worldwide, new challenges arise for system operators in both regulated and deregulated electricity markets to solve the security-constrained coal-fired unit commitment problem with intermittent generation (due to renewables) and uncertain load, in order to ensure system reliability and maintain cost effectiveness. In this paper, we study a security-constrained coal-fired stochastic unit commitment model, which we use to enhance the reliability unit commitment process for day-ahead power system operations. In our approach, we first develop a deterministic equivalent formulation for the problem, which leads to a large-scale mixed-integer linear program.more » Then, we verify that the turn on/off inequalities provide a convex hull representation of the minimum-up/down time polytope under the stochastic setting. Next, we develop several families of strong valid inequalities mainly through lifting schemes. In particular, by exploring sequence independent lifting and subadditive approximation lifting properties for the lifting schemes, we obtain strong valid inequalities for the ramping and general load balance polytopes. Lastly, branch-and-cut algorithms are developed to employ these valid inequalities as cutting planes to solve the problem. Our computational results verify the effectiveness of the proposed approach.« less

Cutting planes for the multistage stochastic unit commitment problem

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jiang, Ruiwei; Guan, Yongpei; Watson, Jean -Paul

As renewable energy penetration rates continue to increase in power systems worldwide, new challenges arise for system operators in both regulated and deregulated electricity markets to solve the security-constrained coal-fired unit commitment problem with intermittent generation (due to renewables) and uncertain load, in order to ensure system reliability and maintain cost effectiveness. In this paper, we study a security-constrained coal-fired stochastic unit commitment model, which we use to enhance the reliability unit commitment process for day-ahead power system operations. In our approach, we first develop a deterministic equivalent formulation for the problem, which leads to a large-scale mixed-integer linear program.more » Then, we verify that the turn on/off inequalities provide a convex hull representation of the minimum-up/down time polytope under the stochastic setting. Next, we develop several families of strong valid inequalities mainly through lifting schemes. In particular, by exploring sequence independent lifting and subadditive approximation lifting properties for the lifting schemes, we obtain strong valid inequalities for the ramping and general load balance polytopes. Lastly, branch-and-cut algorithms are developed to employ these valid inequalities as cutting planes to solve the problem. Our computational results verify the effectiveness of the proposed approach.« less

Hydrodynamical models of cometary H II regions

NASA Astrophysics Data System (ADS)

Steggles, H. G.; Hoare, M. G.; Pittard, J. M.

2017-04-01

We have modelled the evolution of cometary H II regions produced by zero-age main-sequence stars of O and B spectral types, which are driving strong winds and are born off-centre from spherically symmetric cores with power-law (α = 2) density slopes. A model parameter grid was produced that spans stellar mass, age and core density. Exploring this parameter space, we investigated limb-brightening, a feature commonly seen in cometary H II regions. We found that stars with mass M⋆ ≥ 12 M⊙ produce this feature. Our models have a cavity bounded by a contact discontinuity separating hot shocked wind and ionized ambient gas that is similar in size to the surrounding H II region. Because of early pressure confinement, we did not see shocks outside of the contact discontinuity for stars with M⋆ ≤ 40 M⊙, but the cavities were found to continue to grow. The cavity size in each model plateaus as the H II region stagnates. The spectral energy distributions of our models are similar to those from identical stars evolving in uniform density fields. The turn-over frequency is slightly lower in our power-law models as a result of a higher proportion of low-density gas covered by the H II regions.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Skillman, Evan D.; Hidalgo, Sebastian L.; Monelli, Matteo

We present an analysis of the star formation history (SFH) of a field near the half-light radius in the Local Group dwarf irregular galaxy IC 1613 based on deep Hubble Space Telescope Advanced Camera for Surveys imaging. Our observations reach the oldest main sequence turn-off, allowing a time resolution at the oldest ages of ∼1 Gyr. Our analysis shows that the SFH of the observed field in IC 1613 is consistent with being constant over the entire lifetime of the galaxy. These observations rule out an early dominant episode of star formation in IC 1613. We compare the SFH ofmore » IC 1613 with expectations from cosmological models. Since most of the mass is in place at early times for low-mass halos, a naive expectation is that most of the star formation should have taken place at early times. Models in which star formation follows mass accretion result in too many stars formed early and gas mass fractions that are too low today (the 'over-cooling problem'). The depth of the present photometry of IC 1613 shows that, at a resolution of ∼1 Gyr, the star formation rate is consistent with being constant, at even the earliest times, which is difficult to achieve in models where star formation follows mass assembly.« less

Euler Calculations at Off-Design Conditions for an Inlet of Inward Turning RBCC-SSTO Vehicle

NASA Technical Reports Server (NTRS)

Takashima, N.; Kothari, A. P.

1998-01-01

The inviscid performance of an inward turning inlet design is calculated computationally for the first time. Hypersonic vehicle designs based on the inward turning inlets have been shown analytically to have increased effective specific impulse and lower heat load than comparably designed vehicles with two-dimensional inlets. The inward turning inlets are designed inversely from inviscid stream surfaces of known flow fields. The computational study is performed on a Mach 12 inlet design to validate the performance predicted by the design code (HAVDAC) and calculate its off-design Mach number performance. The three-dimensional Euler equations are solved for Mach 4, 8, and 12 using a software package called SAM, which consists of an unstructured mesh generator (SAMmesh), a three-dimensional unstructured mesh flow solver (SAMcfd), and a CAD-based software (SAMcad). The computed momentum averaged inlet throat pressure is within 6% of the design inlet throat pressure. The mass-flux at the inlet throat is also within 7 % of the value predicted by the design code thereby validating the accuracy of the design code. The off-design Mach number results show that flow spillage is minimal, and the variation in the mass capture ratio with Mach number is comparable to an ideal 2-D inlet. The results from the inviscid flow calculations of a Mach 12 inward turning inlet indicate that the inlet design has very good on and off-design performance which makes it a promising design candidate for future air-breathing hypersonic vehicles.

The effects of processing and sequence organization on the timing of turn taking: a corpus study

PubMed Central

Roberts, Seán G.; Torreira, Francisco; Levinson, Stephen C.

2015-01-01

The timing of turn taking in conversation is extremely rapid given the cognitive demands on speakers to comprehend, plan and execute turns in real time. Findings from psycholinguistics predict that the timing of turn taking is influenced by demands on processing, such as word frequency or syntactic complexity. An alternative view comes from the field of conversation analysis, which predicts that the rules of turn-taking and sequence organization may dictate the variation in gap durations (e.g., the functional role of each turn in communication). In this paper, we estimate the role of these two different kinds of factors in determining the speed of turn-taking in conversation. We use the Switchboard corpus of English telephone conversation, already richly annotated for syntactic structure speech act sequences, and segmental alignment. To this we add further information including Floor Transfer Offset (the amount of time between the end of one turn and the beginning of the next), word frequency, concreteness, and surprisal values. We then apply a novel statistical framework (“random forests”) to show that these two dimensions are interwoven together with indexical properties of the speakers as explanatory factors determining the speed of response. We conclude that an explanation of the of the timing of turn taking will require insights from both processing and sequence organization. PMID:26029125

Galactic googly: the rotation-metallicity bias in the inner stellar halo of the Milky Way

NASA Astrophysics Data System (ADS)

Kafle, Prajwal R.; Sharma, Sanjib; Robotham, Aaron S. G.; Pradhan, Raj K.; Guglielmo, Magda; Davies, Luke J. M.; Driver, Simon P.

2017-09-01

The first and second moments of stellar velocities encode important information about the formation history of the Galactic halo. However, due to the lack of tangential motion and inaccurate distances of the halo stars, the velocity moments in the Galactic halo have largely remained 'known unknowns'. Fortunately, our off-centric position within the Galaxy allows us to estimate these moments in the galactocentric frame using the observed radial velocities of the stars alone. We use these velocities coupled with the hierarchical Bayesian scheme, which allows easy marginalization over the missing data (the proper motion, and uncertainty-free distance and line-of-sight velocity), to measure the velocity dispersions, orbital anisotropy (β) and streaming motion (vrot) of the halo main-sequence turn-off (MSTO) and K-giant (KG) stars in the inner stellar halo (r ≲ 15 kpc). We study the metallicity bias in kinematics of the halo stars and observe that the comparatively metal-rich ([Fe/H] > -1.4) and the metal-poor ([Fe/H] ≤ -1.4) MSTO samples show a clear systematic difference in vrot ˜ 20-40 km s - 1, depending on how restrictive the spatial cuts to cull the disc contamination are. The bias is also detected in KG samples but with less certainty. Both MSTO and KG populations suggest that the inner stellar halo of the Galaxy is radially biased I.e. σr > σθ or σϕ and β ≃ 0.5. The apparent metallicity contrariety in the rotation velocity among the halo sub-populations supports the co-existence of multiple populations in the galactic halo that may have formed through distinct formation scenarios, I.e. in situ versus accretion.

Methods for magnetic resonance analysis using magic angle technique

DOEpatents

Hu, Jian Zhi [Richland, WA; Wind, Robert A [Kennewick, WA; Minard, Kevin R [Kennewick, WA; Majors, Paul D [Kennewick, WA

2011-11-22

Methods of performing a magnetic resonance analysis of a biological object are disclosed that include placing the object in a main magnetic field (that has a static field direction) and in a radio frequency field; rotating the object at a frequency of less than about 100 Hz around an axis positioned at an angle of about 54.degree.44' relative to the main magnetic static field direction; pulsing the radio frequency to provide a sequence that includes a phase-corrected magic angle turning pulse segment; and collecting data generated by the pulsed radio frequency. In particular embodiments the method includes pulsing the radio frequency to provide at least two of a spatially selective read pulse, a spatially selective phase pulse, and a spatially selective storage pulse. Further disclosed methods provide pulse sequences that provide extended imaging capabilities, such as chemical shift imaging or multiple-voxel data acquisition.

Analysis of transistor and snubber turn-off dynamics in high-frequency high-voltage high-power converters

NASA Technical Reports Server (NTRS)

Wilson, P. M.; Wilson, T. G.; Owen, H. A., Jr.

1982-01-01

Dc to dc converters which operate reliably and efficiently at switching frequencies high enough to effect substantial reductions in the size and weight of converter energy storage elements are studied. A two winding current or voltage stepup (buck boost) dc-to-dc converter power stage submodule designed to operate in the 2.5-kW range, with an input voltage range of 110 to 180 V dc, and an output voltage of 250 V dc is emphasized. In order to assess the limitations of present day component and circuit technologies, a design goal switching frequency of 10 kHz was maintained. The converter design requirements represent a unique combination of high frequency, high voltage, and high power operation. The turn off dynamics of the primary circuit power switching transistor and its associated turn off snubber circuitry are investigated.

Analysis of transistor and snubber turn-off dynamics in high-frequency high-voltage high-power converters

NASA Astrophysics Data System (ADS)

Wilson, P. M.; Wilson, T. G.; Owen, H. A., Jr.

Dc to dc converters which operate reliably and efficiently at switching frequencies high enough to effect substantial reductions in the size and weight of converter energy storage elements are studied. A two winding current or voltage stepup (buck boost) dc-to-dc converter power stage submodule designed to operate in the 2.5-kW range, with an input voltage range of 110 to 180 V dc, and an output voltage of 250 V dc is emphasized. In order to assess the limitations of present day component and circuit technologies, a design goal switching frequency of 10 kHz was maintained. The converter design requirements represent a unique combination of high frequency, high voltage, and high power operation. The turn off dynamics of the primary circuit power switching transistor and its associated turn off snubber circuitry are investigated.

Composition-dependent nanoelectronics of amido-phenazines: non-volatile RRAM and WORM memory devices.

PubMed

Maiti, Dilip K; Debnath, Sudipto; Nawaz, Sk Masum; Dey, Bapi; Dinda, Enakhi; Roy, Dipanwita; Ray, Sudipta; Mallik, Abhijit; Hussain, Syed A

2017-10-17

A metal-free three component cyclization reaction with amidation is devised for direct synthesis of DFT-designed amido-phenazine derivative bearing noncovalent gluing interactions to fabricate organic nanomaterials. Composition-dependent organic nanoelectronics for nonvolatile memory devices are discovered using mixed phenazine-stearic acid (SA) nanomaterials. We discovered simultaneous two different types of nonmagnetic and non-moisture sensitive switching resistance properties of fabricated devices utilizing mixed organic nanomaterials: (a) sample-1(8:SA = 1:3) is initially off, turning on at a threshold, but it does not turn off again with the application of any voltage, and (b) sample-2 (8:SA = 3:1) is initially off, turning on at a sharp threshold and off again by reversing the polarity. No negative differential resistance is observed in either type. These samples have different device implementations: sample-1 is attractive for write-once-read-many-times memory devices, such as novel non-editable database, archival memory, electronic voting, radio frequency identification, sample-2 is useful for resistive-switching random access memory application.

Improving energy efficiency of Embedded DRAM Caches for High-end Computing Systems

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mittal, Sparsh; Vetter, Jeffrey S; Li, Dong

2014-01-01

With increasing system core-count, the size of last level cache (LLC) has increased and since SRAM consumes high leakage power, power consumption of LLCs is becoming a significant fraction of processor power consumption. To address this, researchers have used embedded DRAM (eDRAM) LLCs which consume low-leakage power. However, eDRAM caches consume a significant amount of energy in the form of refresh energy. In this paper, we propose ESTEEM, an energy saving technique for embedded DRAM caches. ESTEEM uses dynamic cache reconfiguration to turn-off a portion of the cache to save both leakage and refresh energy. It logically divides the cachemore » sets into multiple modules and turns-off possibly different number of ways in each module. Microarchitectural simulations confirm that ESTEEM is effective in improving performance and energy efficiency and provides better results compared to a recently-proposed eDRAM cache energy saving technique, namely Refrint. For single and dual-core simulations, the average saving in memory subsystem (LLC+main memory) on using ESTEEM is 25.8% and 32.6%, respectively and average weighted speedup are 1.09X and 1.22X, respectively. Additional experiments confirm that ESTEEM works well for a wide-range of system parameters.« less

A novel hybrid method of beta-turn identification in protein using binary logistic regression and neural network

PubMed Central

Asghari, Mehdi Poursheikhali; Hayatshahi, Sayyed Hamed Sadat; Abdolmaleki, Parviz

2012-01-01

From both the structural and functional points of view, β-turns play important biological roles in proteins. In the present study, a novel two-stage hybrid procedure has been developed to identify β-turns in proteins. Binary logistic regression was initially used for the first time to select significant sequence parameters in identification of β-turns due to a re-substitution test procedure. Sequence parameters were consisted of 80 amino acid positional occurrences and 20 amino acid percentages in sequence. Among these parameters, the most significant ones which were selected by binary logistic regression model, were percentages of Gly, Ser and the occurrence of Asn in position i+2, respectively, in sequence. These significant parameters have the highest effect on the constitution of a β-turn sequence. A neural network model was then constructed and fed by the parameters selected by binary logistic regression to build a hybrid predictor. The networks have been trained and tested on a non-homologous dataset of 565 protein chains. With applying a nine fold cross-validation test on the dataset, the network reached an overall accuracy (Qtotal) of 74, which is comparable with results of the other β-turn prediction methods. In conclusion, this study proves that the parameter selection ability of binary logistic regression together with the prediction capability of neural networks lead to the development of more precise models for identifying β-turns in proteins. PMID:27418910

A novel hybrid method of beta-turn identification in protein using binary logistic regression and neural network.

PubMed

Asghari, Mehdi Poursheikhali; Hayatshahi, Sayyed Hamed Sadat; Abdolmaleki, Parviz

2012-01-01

From both the structural and functional points of view, β-turns play important biological roles in proteins. In the present study, a novel two-stage hybrid procedure has been developed to identify β-turns in proteins. Binary logistic regression was initially used for the first time to select significant sequence parameters in identification of β-turns due to a re-substitution test procedure. Sequence parameters were consisted of 80 amino acid positional occurrences and 20 amino acid percentages in sequence. Among these parameters, the most significant ones which were selected by binary logistic regression model, were percentages of Gly, Ser and the occurrence of Asn in position i+2, respectively, in sequence. These significant parameters have the highest effect on the constitution of a β-turn sequence. A neural network model was then constructed and fed by the parameters selected by binary logistic regression to build a hybrid predictor. The networks have been trained and tested on a non-homologous dataset of 565 protein chains. With applying a nine fold cross-validation test on the dataset, the network reached an overall accuracy (Qtotal) of 74, which is comparable with results of the other β-turn prediction methods. In conclusion, this study proves that the parameter selection ability of binary logistic regression together with the prediction capability of neural networks lead to the development of more precise models for identifying β-turns in proteins.

Identification and characterization of earthquake clusters: a comparative analysis for selected sequences in Italy

NASA Astrophysics Data System (ADS)

Peresan, Antonella; Gentili, Stefania

2017-04-01

Identification and statistical characterization of seismic clusters may provide useful insights about the features of seismic energy release and their relation to physical properties of the crust within a given region. Moreover, a number of studies based on spatio-temporal analysis of main-shocks occurrence require preliminary declustering of the earthquake catalogs. Since various methods, relying on different physical/statistical assumptions, may lead to diverse classifications of earthquakes into main events and related events, we aim to investigate the classification differences among different declustering techniques. Accordingly, a formal selection and comparative analysis of earthquake clusters is carried out for the most relevant earthquakes in North-Eastern Italy, as reported in the local OGS-CRS bulletins, compiled at the National Institute of Oceanography and Experimental Geophysics since 1977. The comparison is then extended to selected earthquake sequences associated with a different seismotectonic setting, namely to events that occurred in the region struck by the recent Central Italy destructive earthquakes, making use of INGV data. Various techniques, ranging from classical space-time windows methods to ad hoc manual identification of aftershocks, are applied for detection of earthquake clusters. In particular, a statistical method based on nearest-neighbor distances of events in space-time-energy domain, is considered. Results from clusters identification by the nearest-neighbor method turn out quite robust with respect to the time span of the input catalogue, as well as to minimum magnitude cutoff. The identified clusters for the largest events reported in North-Eastern Italy since 1977 are well consistent with those reported in earlier studies, which were aimed at detailed manual aftershocks identification. The study shows that the data-driven approach, based on the nearest-neighbor distances, can be satisfactorily applied to decompose the seismic catalog into background seismicity and individual sequences of earthquake clusters, also in areas characterized by moderate seismic activity, where the standard declustering techniques may turn out rather gross approximations. With these results acquired, the main statistical features of seismic clusters are explored, including complex interdependence of related events, with the aim to characterize the space-time patterns of earthquakes occurrence in North-Eastern Italy and capture their basic differences with Central Italy sequences.

Jordanian pharmaceutical companies: are their marketing efforts paying off?

PubMed

Al-Shaikh, Mustafa S; Torres, Ivonne M; Zuniga, Miguel A; Ghunaim, Ayman

2011-04-01

The pharmaceuticals industry is one of the main industries in Jordan. Jordanian pharmaceuticals rank third in the export industry of this country. This study aims to examine the strengths that Jordanian pharmaceutical companies have, which, in turn, form their competitiveness base. In addition, this study aims to identify their weaknesses and the effects of marketing their products in the local market. What is the relationship between Jordanian pharmaceutical product quality, price and value, and the competitiveness of pharmaceutical companies in the local market? Our study aims to answer this and other questions. Our results and practical implications are discussed.

RADIO EMISSION FROM RED-GIANT HOT JUPITERS

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fujii, Yuka; Spiegel, David S.; Mroczkowski, Tony

2016-04-01

When planet-hosting stars evolve off the main sequence and go through the red-giant branch, the stars become orders of magnitudes more luminous and, at the same time, lose mass at much higher rates than their main-sequence counterparts. Accordingly, if planetary companions exist around these stars at orbital distances of a few au, they will be heated up to the level of canonical hot Jupiters and also be subjected to a dense stellar wind. Given that magnetized planets interacting with stellar winds emit radio waves, such “Red-Giant Hot Jupiters” (RGHJs) may also be candidate radio emitters. We estimate the spectral auroralmore » radio intensity of RGHJs based on the empirical relation with the stellar wind as well as a proposed scaling for planetary magnetic fields. RGHJs might be intrinsically as bright as or brighter than canonical hot Jupiters and about 100 times brighter than equivalent objects around main-sequence stars. We examine the capabilities of low-frequency radio observatories to detect this emission and find that the signal from an RGHJ may be detectable at distances up to a few hundred parsecs with the Square Kilometer Array.« less

A CI-Independent Form of Replicative Inhibition: Turn Off of Early Replication of Bacteriophage Lambda

PubMed Central

Hayes, Sidney; Horbay, Monique A.; Hayes, Connie

2012-01-01

Several earlier studies have described an unusual exclusion phenotype exhibited by cells with plasmids carrying a portion of the replication region of phage lambda. Cells exhibiting this inhibition phenotype (IP) prevent the plating of homo-immune and hybrid hetero-immune lambdoid phages. We have attempted to define aspects of IP, and show that it is directed to repλ phages. IP was observed in cells with plasmids containing a λ DNA fragment including oop, encoding a short OOP micro RNA, and part of the lambda origin of replication, oriλ, defined by iteron sequences ITN1-4 and an adjacent high AT-rich sequence. Transcription of the intact oop sequence from its promoter, pO is required for IP, as are iterons ITN3–4, but not the high AT-rich portion of oriλ. The results suggest that IP silencing is directed to theta mode replication initiation from an infecting repλ genome, or an induced repλ prophage. Phage mutations suppressing IP, i.e., Sip, map within, or adjacent to cro or in O, or both. Our results for plasmid based IP suggest the hypothesis that there is a natural mechanism for silencing early theta-mode replication initiation, i.e. the buildup of λ genomes with oop + oriλ+ sequence. PMID:22590552

Kinematic, kinetic and EMG analysis of four front crawl flip turn techniques.

PubMed

Pereira, Suzana Matheus; Ruschel, Caroline; Hubert, Marcel; Machado, Leandro; Roesler, Helio; Fernandes, Ricardo Jorge; Vilas-Boas, João Paulo

2015-01-01

This study aimed to analyse the kinematic, kinetic and electromyographic characteristics of four front crawl flip turn technique variants. The variants distinguished from each other by differences in body position (i.e., dorsal, lateral, ventral) during rolling, wall support, pushing and gliding phases. Seventeen highly trained swimmers (17.9 ± 3.2 years old) participated in interventional sessions and performed three trials of each variant, being monitored with a 3-D video system, a force platform and an electromyography (EMG) system. Studied variables: rolling time and distance, wall support time, push-off time, peak force and horizontal impulse at wall support and push-off, centre of mass horizontal velocity at the end of the push-off, gliding time, centre of mass depth, distance, average and final velocity during gliding, total turn time and electrical activity of Gastrocnemius Medialis, Tibialis Anterior, Biceps Femoris and Vastus Lateralis muscles. Depending on the variant, total turn time ranged from 2.37 ± 0.32 to 2.43 ± 0.33 s, push-off force from 1.86 ± 0.33 to 1.92 ± 0.26 BW and centre of mass velocity during gliding from 1.78 ± 0.21 to 1.94 ± 0.22 m · s(-1). The variants were not distinguishable in terms of kinematical, kinetic and EMG parameters during the rolling, wall support, pushing and gliding phases.

Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lichius, Alexander; Bidard, Frédérique; Buchholz, Franziska

2015-04-20

Trichoderma reesei is the main industrial source of cellulases and hemicellulases required for the hydrolysis of biomass to simple sugars, which can then be used in the production of biofuels and biorefineries. The highly productive strains in use today were generated by classical mutagenesis. As byproducts of this procedure, mutants were generated that turned out to be unable to produce cellulases. In order to identify the mutations responsible for this inability, we sequenced the genome of one of these strains, QM9136, and compared it to that of its progenitor T. reesei QM6a.

First report of the epiphytic dinoflagellate Gambierdiscus caribaeus in the temperate waters off Jeju Island, Korea: morphology and molecular characterization.

PubMed

Jeong, Hae Jin; Lim, An Suk; Jang, Se Hyeon; Yih, Won Ho; Kang, Nam Seon; Lee, Sung Yeon; Yoo, Yeong Du; Kim, Hyung Seop

2012-01-01

Gambierdiscus spp. are epiphytic, benthic dinoflagellates. Some species have been shown to be toxic and cause ciguatera fish poisoning. We report, for the first time, the occurrence of Gambierdiscus caribaeus isolated from the waters off Jeju Island in Korea. Its morphology was similar to that of the original Belize strains of G. caribaeus. Gambierdiscus caribaeus has been reported in the tropical and subtropical waters of the Pacific, Gulf of Mexico, Caribbean Sea, and Floridian coast. Our report extends its range to the North Pacific Ocean. The plates of the Korean strain were arranged in a Kofoidian series of Po, 3', 7'', 6c, 6s, 5''', 1p, and 2'''', morphologically closer to other strains of G. caribaeus than to G. carpenteri. When properly aligned, its small subunit (SSU) rDNA was 0.5% different from those of Gambierdiscus sp. C-1, a strain that was isolated from the waters off eastern Japan, but was 2.4-4.0% different from those of the NOAA strains of G. caribaeus and 3.1-3.4% different from those of the NOAA strains of G. carpenteri. Additionally, the D1-D3 large subunit (LSU) rDNA sequence of the Korean strain of G. caribaeus was 4.7-5.3% different from those of the NOAA strains of G. caribaeus and 7.1-7.5% different from those of all reported G. carpenteri strains, including the NOAA strains. In phylogenetic trees based on SSU and LSU rDNA sequences, our Korean strain was basal to the clade consisting of the NOAA strains of G. caribaeus, which in turn was sister clade to all reported G. carpenteri strains. © 2012 The Author(s) Journal of Eukaryotic Microbiology © 2012 International Society of Protistologists.

Brain-wide mapping of neural activity controlling zebrafish exploratory locomotion

PubMed Central

Dunn, Timothy W; Mu, Yu; Narayan, Sujatha; Randlett, Owen; Naumann, Eva A; Yang, Chao-Tsung; Schier, Alexander F

2016-01-01

In the absence of salient sensory cues to guide behavior, animals must still execute sequences of motor actions in order to forage and explore. How such successive motor actions are coordinated to form global locomotion trajectories is unknown. We mapped the structure of larval zebrafish swim trajectories in homogeneous environments and found that trajectories were characterized by alternating sequences of repeated turns to the left and to the right. Using whole-brain light-sheet imaging, we identified activity relating to the behavior in specific neural populations that we termed the anterior rhombencephalic turning region (ARTR). ARTR perturbations biased swim direction and reduced the dependence of turn direction on turn history, indicating that the ARTR is part of a network generating the temporal correlations in turn direction. We also find suggestive evidence for ARTR mutual inhibition and ARTR projections to premotor neurons. Finally, simulations suggest the observed turn sequences may underlie efficient exploration of local environments. DOI: http://dx.doi.org/10.7554/eLife.12741.001 PMID:27003593

Rapid synthesis of N, S co-doped carbon dots and their application for Fe3+ ion detection

NASA Astrophysics Data System (ADS)

Zhang, Jian; Wang, Junbin; Fu, Jinping; Fu, Xucheng; Gan, Wei; Hao, Hequn

2018-02-01

In this study, nitrogen and sulfur co-doped carbon dots (NSCDs) were fabricated by microwave-assisted one-pot synthesis using vitamin C and thiourea as precursors. The as-prepared NSCDs demonstrated excellent properties, including aqueous dispensability, strong fluorescence emission, excellent environmental stability, high selectivity, and sensitivity toward Fe3+ ions. The NSCD-based material can be used as a "turn off" fluorescent probe for detecting Fe3+ ions at a low detection limit (4.2 nM). In addition, the "turn off" and "turn on" of NSCD fluorescent probe could be modulated by adding Fe3+ and EDTA, indicating weak interaction between the Fe3+ ions and NSCDs.

36 CFR 7.75 - Padre Island National Seashore.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) Off-road motor vehicle and motorcycle operation. (1) The following regulations pertain to the operation of motor vehicles and motorcycles off established roads and parking areas. The operation of such... turning out of the track to the right. (2) Off-road motor vehicle and motorcycle use areas and routes. The...

36 CFR 7.75 - Padre Island National Seashore.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) Off-road motor vehicle and motorcycle operation. (1) The following regulations pertain to the operation of motor vehicles and motorcycles off established roads and parking areas. The operation of such... turning out of the track to the right. (2) Off-road motor vehicle and motorcycle use areas and routes. The...

36 CFR 7.75 - Padre Island National Seashore.

Code of Federal Regulations, 2011 CFR

2011-07-01

...) Off-road motor vehicle and motorcycle operation. (1) The following regulations pertain to the operation of motor vehicles and motorcycles off established roads and parking areas. The operation of such... turning out of the track to the right. (2) Off-road motor vehicle and motorcycle use areas and routes. The...

36 CFR 7.75 - Padre Island National Seashore.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) Off-road motor vehicle and motorcycle operation. (1) The following regulations pertain to the operation of motor vehicles and motorcycles off established roads and parking areas. The operation of such... turning out of the track to the right. (2) Off-road motor vehicle and motorcycle use areas and routes. The...

36 CFR 7.75 - Padre Island National Seashore.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) Off-road motor vehicle and motorcycle operation. (1) The following regulations pertain to the operation of motor vehicles and motorcycles off established roads and parking areas. The operation of such... turning out of the track to the right. (2) Off-road motor vehicle and motorcycle use areas and routes. The...

49 CFR 595.5 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

..., DEPARTMENT OF TRANSPORTATION (CONTINUED) MAKE INOPERATIVE EXEMPTIONS Retrofit On-Off Switches for Air Bags... an occupant of the vehicle to turn off an air bag in that vehicle, subject to the conditions in... Administration that authorizes the installation of an on-off switch in that vehicle for that air bag and includes...

49 CFR 595.5 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

..., DEPARTMENT OF TRANSPORTATION (CONTINUED) MAKE INOPERATIVE EXEMPTIONS Retrofit On-Off Switches for Air Bags... an occupant of the vehicle to turn off an air bag in that vehicle, subject to the conditions in... Administration that authorizes the installation of an on-off switch in that vehicle for that air bag and includes...

49 CFR 595.5 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

..., DEPARTMENT OF TRANSPORTATION (CONTINUED) MAKE INOPERATIVE EXEMPTIONS Retrofit On-Off Switches for Air Bags... an occupant of the vehicle to turn off an air bag in that vehicle, subject to the conditions in... Administration that authorizes the installation of an on-off switch in that vehicle for that air bag and includes...

Design of Automatic Intensity Varying Smart Street Lighting System

NASA Astrophysics Data System (ADS)

Gupta, Ashutosh; Gupta, Shipra

2017-08-01

The paper is proposed with an aim of power conservation. In this era of development, it is essential to develop a streetlight that turns on and off automatically without human interference. To achieve this light sensor have been placed in each panel which turns the street light on and off automatically. For energy conservation cool-white LED’s have been used in street light panel and dimmer modules have been installed which changes the intensity of the streetlight depending on the darkness.

Overload protection system for power inverter

NASA Technical Reports Server (NTRS)

Nagano, S. (Inventor)

1977-01-01

An overload protection system for a power inverter utilized a first circuit for monitoring current to the load from the power inverter to detect an overload and a control circuit to shut off the power inverter, when an overload condition was detected. At the same time, a monitoring current inverter was turned on to deliver current to the load at a very low power level. A second circuit monitored current to the load, from the monitoring current inverter, to hold the power inverter off through the control circuit, until the overload condition was cleared so that the control circuit may be deactivated in order for the power inverter to be restored after the monitoring current inverter is turned off completely.

Genetics Home Reference: Liebenberg syndrome

MedlinePlus

... changes affect regions of DNA known as regulatory elements, which help turn on or turn off genes ( ... Citation on PubMed or Free article on PubMed Central Seoighe DM, Gadancheva V, Regan R, McDaid J, ...

Burns

MedlinePlus

... doing so puts you in danger as well. Chemical and Electrical Burns For chemical and electrical burns, call 911 or your local ... the power source has been turned off. For chemical burns: Dry chemicals should be brushed off the ...

Flip-Flop Digital Modulator

NASA Technical Reports Server (NTRS)

Eno, R. F.

1984-01-01

Clock switched on and off in response to data signal. Flip-flop modulator generates square-wave carrier frequency that is half clock frequency and turns carrier on and off. Final demodulator output logical inverse of data input.

HMMER Cut-off Threshold Tool (HMMERCTTER): Supervised classification of superfamily protein sequences with a reliable cut-off threshold.

PubMed

Pagnuco, Inti Anabela; Revuelta, María Victoria; Bondino, Hernán Gabriel; Brun, Marcel; Ten Have, Arjen

2018-01-01

Protein superfamilies can be divided into subfamilies of proteins with different functional characteristics. Their sequences can be classified hierarchically, which is part of sequence function assignation. Typically, there are no clear subfamily hallmarks that would allow pattern-based function assignation by which this task is mostly achieved based on the similarity principle. This is hampered by the lack of a score cut-off that is both sensitive and specific. HMMER Cut-off Threshold Tool (HMMERCTTER) adds a reliable cut-off threshold to the popular HMMER. Using a high quality superfamily phylogeny, it clusters a set of training sequences such that the cluster-specific HMMER profiles show cluster or subfamily member detection with 100% precision and recall (P&R), thereby generating a specific threshold as inclusion cut-off. Profiles and thresholds are then used as classifiers to screen a target dataset. Iterative inclusion of novel sequences to groups and the corresponding HMMER profiles results in high sensitivity while specificity is maintained by imposing 100% P&R self detection. In three presented case studies of protein superfamilies, classification of large datasets with 100% precision was achieved with over 95% recall. Limits and caveats are presented and explained. HMMERCTTER is a promising protein superfamily sequence classifier provided high quality training datasets are used. It provides a decision support system that aids in the difficult task of sequence function assignation in the twilight zone of sequence similarity. All relevant data and source codes are available from the Github repository at the following URL: https://github.com/BBCMdP/HMMERCTTER.

HMMER Cut-off Threshold Tool (HMMERCTTER): Supervised classification of superfamily protein sequences with a reliable cut-off threshold

PubMed Central

Pagnuco, Inti Anabela; Revuelta, María Victoria; Bondino, Hernán Gabriel; Brun, Marcel

2018-01-01

Background Protein superfamilies can be divided into subfamilies of proteins with different functional characteristics. Their sequences can be classified hierarchically, which is part of sequence function assignation. Typically, there are no clear subfamily hallmarks that would allow pattern-based function assignation by which this task is mostly achieved based on the similarity principle. This is hampered by the lack of a score cut-off that is both sensitive and specific. Results HMMER Cut-off Threshold Tool (HMMERCTTER) adds a reliable cut-off threshold to the popular HMMER. Using a high quality superfamily phylogeny, it clusters a set of training sequences such that the cluster-specific HMMER profiles show cluster or subfamily member detection with 100% precision and recall (P&R), thereby generating a specific threshold as inclusion cut-off. Profiles and thresholds are then used as classifiers to screen a target dataset. Iterative inclusion of novel sequences to groups and the corresponding HMMER profiles results in high sensitivity while specificity is maintained by imposing 100% P&R self detection. In three presented case studies of protein superfamilies, classification of large datasets with 100% precision was achieved with over 95% recall. Limits and caveats are presented and explained. Conclusions HMMERCTTER is a promising protein superfamily sequence classifier provided high quality training datasets are used. It provides a decision support system that aids in the difficult task of sequence function assignation in the twilight zone of sequence similarity. All relevant data and source codes are available from the Github repository at the following URL: https://github.com/BBCMdP/HMMERCTTER. PMID:29579071

Diversity of culturable filamentous Ascomycetes in the eastern South Pacific Ocean off Chile.

PubMed

Vera, Jeanett; Gutiérrez, Marcelo H; Palfner, Götz; Pantoja, Silvio

2017-08-01

Our study reports the diversity of culturable mycoplankton in the eastern South Pacific Ocean off Chile to contribute with novel knowledge on taxonomy of filamentous fungi isolated from distinct physicochemical and biological marine environments. We characterized spatial distribution of isolates, evaluated their viability and assessed the influence of organic substrate availability on fungal development. Thirty-nine Operational Taxonomic Units were identified from 99 fungal strains isolated from coastal and oceanic waters by using Automatic Barcode Gap Discovery. All Operational Taxonomic Units belonged to phylum Ascomycota and orders Eurotiales, Dothideales, Sordariales and Hypocreales, mainly Penicillium sp. (82%); 11 sequences did not match existing species in GenBank, suggesting occurrence of novel fungal taxa. Our results suggest that fungal communities in the South Pacific Ocean off Chile appear to thrive in a wide range of environmental conditions in the ocean and that substrate availability may be a factor influencing fungal viability in the ocean.

Sequence stratigraphic control on prolific HC reservoir development, Southwest Iran

USGS Publications Warehouse

Lasemi, Y.; Kondroud, K.N.

2008-01-01

An important carbonate formation in the Persian Gulf and the onshore oil fields of Southwest Iran is the Lowermost Cretaceous Fahliyan formation. The formation in Darkhowain field consists of unconformity-bounded depositional sequences containing prolific hydrocarbon reservoirs of contrasting origin. Located in the high stand systems tract (HST) of the lower sequence encompassing over 200m of oil column are the most prolific reservoir. Another reservoir is over 80m thick consisting of shallowing-upward cycles that are best developed within the transgressive systems tract of the upper sequence. Vertical facies distribution and their paleobathymetry and geophysical log signatures of the Fahliyan formation in the Darkhowain platform reveal the presence of two unconformity-bounded depositional sequences in Vail et al., Van Wagoner et al., and Sarg. The Fahliyan formation mainly consists of platform carbonates composed of restricted bioclastic lime mudstone to packstone of the platform interior, Lithocodium boundstone or ooid-intraclast-bioclast grainstone of the high energy platform margin and the bioclast packstone to lime mudstone related to the off-platform setting.

Valorisation of CO2-rich off-gases to biopolymers through biotechnological process.

PubMed

Garcia-Gonzalez, Linsey; De Wever, Heleen

2017-11-01

As one of the key enabling technologies, industrial biotechnology has a high potential to tackle harmful CO2 emissions and to turn CO2 into a valuable commodity. So far, experimental work mainly focused on the bioconversion of pure CO2 to chemicals and plastics and little is known about the tolerance of the bioprocesses to the presence of impurities. This work is the first to investigate the impact of real CO2-rich off-gases on autotrophic production of polyhydroxybutyrate. To this end, two-phase heterotrophic-autotrophic fermentation experiments were set up, consisting of heterothrophic cell mass growth using glucose as substrate followed by autotrophic biopolymer production using either pure synthetic CO2 or industrial off-gases sampled at two point sources. The use of real off-gases did not affect the bacterial performance. High biopolymer content (up to 73%) and productivities (up to 0.227 g/lh) were obtained. Characterisation of the polymers showed that all biopolymers had similar properties, independent of the CO2 source. Moreover, the CO2-derived biopolymers' properties were comparable to commercial ones and biopolymers reported in literature, which are all produced from organic carbon sources. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Aromatic residues engineered into the beta-turn nucleation site of ubiquitin lead to a complex folding landscape, non-native side-chain interactions, and kinetic traps.

PubMed

Rea, Anita M; Simpson, Emma R; Meldrum, Jill K; Williams, Huw E L; Searle, Mark S

2008-12-02

The fast folding of small proteins is likely to be the product of evolutionary pressures that balance the search for native-like contacts in the transition state with the minimum number of stable non-native interactions that could lead to partially folded states prone to aggregation and amyloid formation. We have investigated the effects of non-native interactions on the folding landscape of yeast ubiquitin by introducing aromatic substitutions into the beta-turn region of the N-terminal beta-hairpin, using both the native G-bulged type I turn sequence (TXTGK) as well as an engineered 2:2 XNGK type I' turn sequence. The N-terminal beta-hairpin is a recognized folding nucleation site in ubiquitin. The folding kinetics for wt-Ub (TLTGK) and the type I' turn mutant (TNGK) reveal only a weakly populated intermediate, however, substitution with X = Phe or Trp in either context results in a high propensity to form a stable compact intermediate where the initial U-->I collapse is visible as a distinct kinetic phase. The introduction of Trp into either of the two host turn sequences results in either complex multiphase kinetics with the possibility of parallel folding pathways, or formation of a highly compact I-state stabilized by non-native interactions that must unfold before refolding. Sequence substitutions with aromatic residues within a localized beta-turn capable of forming non-native hydrophobic contacts in both the native state and partially folded states has the undesirable consequence that folding is frustrated by the formation of stable compact intermediates that evolutionary pressures at the sequence level may have largely eliminated.

An Anatomy of a Seismic Sequence in a Deep Gold Mine

NASA Astrophysics Data System (ADS)

Gibowicz, S. J.

1997-12-01

An unusual swarm-like seismic sequence occurred in April 1993 at the Western Deep Levels gold mine, South Africa. Altogether 199 events with moment magnitude from -0.5 to 3.1 were recorded and located by the mine seismic network. The sequence lasted 12 days and was composed in fact of four main shock-aftershocks sequences, closely following each other in space and time. The events were confined to a volume of rock extending to 670 m in the N-S, 630 m in the E-W, and 390 m in the vertical directions. The first sequence lasted 179 hours and the second only 13 hours, being interrupted by the third sequence which lasted 31 hours, being in turn interrupted by the fourth sequence. The parameter p, describing the rate of occurrence of aftershocks, ranged from 0.7 to 1. The first sequence is characterized by the lowest value of the fractal correlation dimension D = 1.75 and the second by the highest value of D = 2.4, whereas the third and fourth sequences are characterized by the middle value of D = 1.9.¶The corner frequencies of P and S waves are in close proximity and range from 14 to 220 Hz. A display of source parameters as a function of time shows that the four main shocks are most distinctly marked by their source radius. For 46 events a moment tensor inversion was performed. In most cases the double-couple component is dominant, ranging from 60 to 90 percent of the solution. The double-couple solutions correspond to the same number of normal and reverse faults and oblique-slip focal mechanisms. An analysis of space distribution of P, T and B axes reveals that the distribution of B axes is the most regular.

Medium dependent dual turn on/turn off fluorescence sensing for Cu2 + ions using AMI/SDS assemblies

NASA Astrophysics Data System (ADS)

Gujar, Varsha B.; Ottoor, Divya

2017-02-01

Behavior of Amiloride (AMI) as a metal ion sensor in anionic surfactant assemblies of varying concentrations at different pH is depicted in this work. From a non-sensor fluorophore, AMI has been transformed in to a tunable fluorosensor for Cu2 + ions in various SDS concentrations. At premicellar concentration of SDS, ion-pair complex is expected to be formed between AMI and SDS due to electrostatic interactions between them. However at CMC concentrations of SDS, fluorescence intensity of AMI is greatly enhanced with red shift in emission, due to the incorporation of AMI molecule in the hydrophobic micellar interface. The behavior of metal sensing by AMI-SDS assemblies gives rise to several interesting observations. Micellation of SDS has been greatly enhanced by increasing copper ion concentrations, as these counter ions screens the charge on monomers of SDS which lead to the aggregation at premicellar concentrations only. Concentrations and pH dependent discrete trends of interactions between SDS-AMI and SDS-Cu2 + ions, have given tunable fluorescence responses (fluorescence turn on/turn off) of AMI for added Cu2 + ions. The electrostatic interaction between the metal cations and the anionic surfactants is the driving force for bringing the metal ions near to the vicinity of micelle where AMI resides. Thus, a comprehensive understanding of the mechanism related to the 'turn on-turn off' fluorescence response of AMI with respect to pH and SDS concentration for effective Cu2 + ion sensing is illustrated in this work.

Crash sequence based risk matrix for motorcycle crashes.

PubMed

Wu, Kun-Feng; Sasidharan, Lekshmi; Thor, Craig P; Chen, Sheng-Yin

2018-04-05

Considerable research has been conducted related to motorcycle and other powered-two-wheeler (PTW) crashes; however, it always has been controversial among practitioners concerning with types of crashes should be first targeted and how to prioritize resources for the implementation of mitigating actions. Therefore, there is a need to identify types of motorcycle crashes that constitute the greatest safety risk to riders - most frequent and most severe crashes. This pilot study seeks exhibit the efficacy of a new approach for prioritizing PTW crash causation sequences as they relate to injury severity to better inform the application of mitigating countermeasures. To accomplish this, the present study constructed a crash sequence-based risk matrix to identify most frequent and most severe motorcycle crashes in an attempt to better connect causes and countermeasures of PTW crashes. Although the frequency of each crash sequence can be computed from crash data, a crash severity model is needed to compare the levels of crash severity among different crash sequences, while controlling for other factors that also have effects on crash severity such drivers' age, use of helmet, etc. The construction of risk matrix based on crash sequences involve two tasks: formulation of crash sequence and the estimation of a mixed-effects (ME) model to adjust the levels of severities for each crash sequence to account for other crash contributing factors that would have an effect on the maximum level of crash severity in a crash. Three data elements from the National Automotive Sampling System - General Estimating System (NASS-GES) data were utilized to form a crash sequence: critical event, crash types, and sequence of events. A mixed-effects model was constructed to model the severity levels for each crash sequence while accounting for the effects of those crash contributing factors on crash severity. A total of 8039 crashes involving 8208 motorcycles occurred during 2011 and 2013 were included in this study, weighted to represent 338,655 motorcyclists involved in traffic crashes in three years (2011-2013)(NHTSA, 2013). The top five most frequent and severe types of crash sequences were identified, accounting for 23 percent of all the motorcycle crashes included in the study, and they are (1) run-off-road crashes on the right, and hitting roadside objects, (2) cross-median crashes, and rollover, (3) left-turn oncoming crashes, and head-on, (4) crossing over (passing through) or turning into opposite direction at intersections, and (5) side-impacted. In addition to crash sequences, several other factors were also identified to have effects on crash severity: use of helmet, presence of horizontal curves, alcohol consumption, road surface condition, roadway functional class, and nighttime condition. Copyright © 2018 Elsevier Ltd. All rights reserved.

The Hubble Space Telescope UV Legacy Survey of Galactic Globular Clusters. XV. The Dynamical Clock: Reading Cluster Dynamical Evolution from the Segregation Level of Blue Straggler Stars

NASA Astrophysics Data System (ADS)

Ferraro, F. R.; Lanzoni, B.; Raso, S.; Nardiello, D.; Dalessandro, E.; Vesperini, E.; Piotto, G.; Pallanca, C.; Beccari, G.; Bellini, A.; Libralato, M.; Anderson, J.; Aparicio, A.; Bedin, L. R.; Cassisi, S.; Milone, A. P.; Ortolani, S.; Renzini, A.; Salaris, M.; van der Marel, R. P.

2018-06-01

The parameter A +, defined as the area enclosed between the cumulative radial distribution of blue straggler stars (BSSs) and that of a reference population, is a powerful indicator of the level of BSS central segregation. As part of the Hubble Space Telescope UV Legacy Survey of Galactic globular clusters (GCs), here we present the BSS population and the determination of A + in 27 GCs observed out to about one half-mass radius. In combination with 21 additional clusters discussed in a previous paper, this provides us with a global sample of 48 systems (corresponding to ∼32% of the Milky Way GC population), for which we find a strong correlation between A + and the ratio of cluster age to the current central relaxation time. Tight relations have also been found with the core radius and the central luminosity density, which are expected to change with the long-term cluster dynamical evolution. An interesting relation is emerging between A + and the ratio of the BSS velocity dispersion relative to that of main sequence turn-off stars, which measures the degree of energy equipartition experienced by BSSs in the cluster. These results provide further confirmation that BSSs are invaluable probes of GC internal dynamics and that A + is a powerful dynamical clock.

Population gradient in the Sextans dSph: comprehensive mapping of a dwarf galaxy by Suprime-Cam

NASA Astrophysics Data System (ADS)

Okamoto, S.; Arimoto, N.; Tolstoy, E.; Jablonka, P.; Irwin, M. J.; Komiyama, Y.; Yamada, Y.; Onodera, M.

2017-05-01

We present the deep and wide V and Ic photometry of the Sextans dwarf spheroidal galaxy (dSph) taken by the Suprime-Cam imager on the Subaru Telescope, which extends out to the tidal radius. The colour-magnitude diagram (CMD) reaches two magnitudes below the main-sequence (MS) turn-off, showing a steep red giant branch, a blue and a red horizontal branch (BHB and RHB, respectively), a sub-giant branch (SGB), an MS and blue stragglers (BSs). We construct the radial profile of each evolutionary phase and demonstrate that blue HB stars are more spatially extended, while red HB stars are more centrally concentrated than the other components. The colour distribution of SGB stars also varies with the galactocentric distance; the inner SGB stars shift bluer than those in the outskirts. The radial differences in the CMD morphology indicate the existence of the age gradient. The relatively younger stars (˜10 Gyr) are more centrally concentrated than the older ones (˜13 Gyr). The spatial contour maps of stars in different age bins also show that the younger population has a higher concentration and higher ellipticity than the older one. We also detect the centrally concentrated bright BS stars, the number of which is consistent with the idea that a part of these stars belongs to the remnant of a disrupted star cluster discovered in the previous spectroscopic studies.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Li, T. S.; Balbinot, E.; Mondrik, N.

We report the discovery of an excess of main sequence turn-off stars in the direction of the constellations of Eridanus and Phoenix from the first year data of the Dark Energy Survey (DES). The Eridanus-Phoenix (EriPhe) overdensity is centered around l~285 deg and b~-60 deg and spans at least 30 deg in longitude and 10 deg in latitude. The Poisson significance of the detection is at least 9 sigma. The stellar population in the overdense region is similar in brightness and color to that of the nearby globular cluster NGC 1261, indicating that the heliocentric distance of EriPhe is aboutmore » d~16 kpc. The extent of EriPhe in projection is therefore at least ~4 kpc by ~3 kpc. On the sky, this overdensity is located between NGC 1261 and a new stellar stream discovered by DES at a similar heliocentric distance, the so-called Phoenix Stream. Given their similar distance and proximity to each other, it is possible that these three structures may be kinematically associated. Alternatively, the EriPhe overdensity is morphologically similar to the Virgo overdensity and the Hercules-Aquila cloud, which also lie at a similar Galactocentric distance. These three overdensities lie along a polar plane separated by ~120 deg and may share a common origin. Spectroscopic follow-up observations of the stars in EriPhe are required to fully understand the nature of this overdensity.« less

Protein function in precision medicine: deep understanding with machine learning.

PubMed

Rost, Burkhard; Radivojac, Predrag; Bromberg, Yana

2016-08-01

Precision medicine and personalized health efforts propose leveraging complex molecular, medical and family history, along with other types of personal data toward better life. We argue that this ambitious objective will require advanced and specialized machine learning solutions. Simply skimming some low-hanging results off the data wealth might have limited potential. Instead, we need to better understand all parts of the system to define medically relevant causes and effects: how do particular sequence variants affect particular proteins and pathways? How do these effects, in turn, cause the health or disease-related phenotype? Toward this end, deeper understanding will not simply diffuse from deeper machine learning, but from more explicit focus on understanding protein function, context-specific protein interaction networks, and impact of variation on both. © 2016 Federation of European Biochemical Societies.

The effect of progressive hypoxia on school structure and dynamics in Atlantic herring Clupea harengus.

PubMed

Domenici, Paolo; Ferrari, R Silvana; Steffensen, John F; Batty, Robert S

2002-10-22

The effect of progressive hypoxia on the structure and dynamics of herring (Clupea harengus) schools in laboratory conditions was investigated. The length, width and depth of schools of about 20 individuals were measured from video recordings to test the hypothesis that during hypoxia fish schools change their shape and volume. School shape (calculated as the ratios of length/depth, width/depth and length/width) did not change significantly during hypoxia. School length, width, depth, area and volume were all significantly increased at 20% oxygen saturation. Volume, area and width were more sensitive to hypoxia; volume and width were also increased at 25% and area at 30% oxygen saturation. The degree of position changing (shuffling) of individuals within the school was also analysed. Shuffling in normoxia was observed to occur largely through 'O-turn' manoeuvres, a 360( degrees )turn executed laterally to the school that allowed fishes in the front to move to the back. O-turn frequency during normoxia was 0.69 O-turns fish(-1) min(-1) but significantly decreased with hypoxia to 0.37 O-turns fish(-1) min(-1) at 30% oxygen saturation. Shuffling was also investigated by measuring the persistence time of individual herring in leading positions (i.e. the first half of the school). No significant changes occurred during hypoxia, indicating that the decrease in O-turn frequency does not affect shuffling rate during hypoxia, and that position shuffling in hypoxic conditions is mainly due to overtaking or falling back by individual fishes. School integrity and positional dynamics are the outcome of trade-offs among a number of biotic factors, such as food, predator defence, mating behaviour and various physical factors that may impose certain limits. Among these, our results indicate that oxygen level modulates schooling behaviour. Oxygen alters whole-school parameters at oxygen saturation values that can be encountered by herring in the field, indicating that oxygen availability is an important factor in the trade-offs that determine school volume. An increase in school volume in the wild may increase the oxygen available to each individual. However, shuffling rate is not affected by hypoxia, indicating that the internal dynamics of positioning is the result of the balance of other factors, for example related to the nutritional state of each individual fish as suggested by previous studies.

[Development of residual voltage testing equipment].

PubMed

Zeng, Xiaohui; Wu, Mingjun; Cao, Li; He, Jinyi; Deng, Zhensheng

2014-07-01

For the existing measurement methods of residual voltage which can't turn the power off at peak voltage exactly and simultaneously display waveforms, a new residual voltage detection method is put forward in this paper. First, the zero point of the power supply is detected with zero cross detection circuit and is inputted to a single-chip microcomputer in the form of pulse signal. Secend, when the zero point delays to the peak voltage, the single-chip microcomputer sends control signal to power off the relay. At last, the waveform of the residual voltage is displayed on a principal computer or oscilloscope. The experimental results show that the device designed in this paper can turn the power off at peak voltage and is able to accurately display the voltage waveform immediately after power off and the standard deviation of the residual voltage is less than 0.2 V at exactly one second and later.

Stratigraphy, geochronology, and accretionary terrane settings of two Bronson Hill arc sequences, northern New England

USGS Publications Warehouse

Moench, R.H.; Aleinikoff, J.N.

2002-01-01

The Ammonoosuc Volcanics, Partridge Formation, and the Oliverian and Highlandcroft Plutonic Suites of the Bronson Hill anticlinorium (BHA) in axial New England are widely accepted as a single Middle to Late Ordovician magmatic arc that was active during closure of Iapetus. Mapping and U-Pb dating indicate, however, that the BHA contains two volcano-sedimentary-intrusive sequences of probable opposite subduction polarity, here termed the Ammonoosuc and Quimby sequences. The Ammonoosuc sequence is defined by the Middle Ordovician Ammonoosuc Volcanics near Littleton, N.H., the type area, northeast to Milan, N.H., and Oquossoc, Me.; it also includes black slate of the Partidge Formation (C. bicornis zone graptolites, ???457 Ma). Related metamorphosed intrusive are the tonalitic Joslin Turn pluton (469 ?? 2 Ma), the Cambridge Black granitic pluton (468 ?? 3 Ma), and gabbro, tonalite (467 ?? 4 Ma), and sheeted diabase of the Chickwolnepy instructions. These intrusives cut lowermost Ammonoosuc (therefore>469 Ma). Probable uppermost Ammonoosuc is dated at 465 ?? 6 and 461 ?? 8 Ma. Successively below the Ammonoosuc are the Dead River and Hurricane Mountain Formations (flysch and melange), and the Jim Pond Formation (484 ?? 5 Ma) and Boil Mountain Complex (both ophiolite), which are structurally underlain by the Neoproterozoic(?) Chain Lakes massif. The Quimby sequence is defined by the Lower Silurian(?) to Upper Ordovician Quimby Formation, composed of bimodal volcanics (443 ?? 4 Ma) and sulfidic shale and graywacke that lie conformably to unconformably above the Ammonoosuc Volcanics and Partridge Formation. Also in the Quimby sequence are several granitic to sparsely gabbroic plutons of the Highlandcroft (441-452 Ma) and Oliverian (435-456 Ma) Plutonic Suites, which intrude the Dead River, Ammonoosuc and Partridge, but not the Quimby Formation. Based on faunal, paleolatitude, and isotropic data, the Ammonoosuc sequence and its correlative and underlying sequences formed off the southern Laurentian margin, but northwest of the principal Iapetan suture, or Red Indian line (RIL). The Boil Mountain-Jim Pond-Hurricane Mountain sequence was ramped northwestward over the Chain Lakes massif at ???475 Ma, on the basal Boil Mountain surface. This obduction probably occurred slightly before obduction on the Baie Verte-Brompton surface (BBL), farther NW, over the Laurentian margin, and was followed by Dead River flysch sedimentation, which ended with the abrupt onset of Ammonoosuc-sequence arc magmatism at ???470 Ma. Ammonoosuc eruptions probably ended at ???460 Ma, when Iapetus closed along the Red Indian line. During a following magmatic hiatus of ???3-5 m.y., now represented by portions of the Partridge Formation that overlie the Ammonoosuc Volcanics, subduction polarity reversed, and subduction resumed below the northwest-dipping Brunswick subduction complex (BSC) of New Brunswick, Canada. Quimby-sequence magmatism (???456-435 Ma) on the the newly accreted Laurentian margin occurred above the BSC, whose footwall is now buried to the southeast by mainly Silurian clastic sediments of the Merrimack-Fredericton trough, deposited in the "Fredericton Sea". In Silurian to Early Devonian time, the NW-dipping BSC footwall was paired with a SE-dipping subduction zone that produced arc magmas of the Coastal Volcanic belt, built on the composite Avalon and adjacent peri-Avalonian terranes. Orogen-normal extension produced by rapid rollback of both subduction zones narrowed the Fredericton Sea, produced the Central Maine and Connecticut Valley-Gaspe?? basins, and culminated in the Acadian orogeny when the sea completely closed in Early Devonian time. Published by Elsevier Science Ltd.

Stratigraphy, geochronology, and accretionary terrane settings of two Bronson Hill arc sequences, northern New England

USGS Publications Warehouse

Moench, R.H.; Aleinikoff, J.N.

2003-01-01

The Ammonoosuc Volcanics, Partridge Formation, and the Oliverian and Highlandcroft Plutonic Suites of the Bronson Hill anticlinorium (BHA) in axial New England are widely accepted as a single Middle to Late Ordovician magmatic arc that was active during closure of Iapetus. Mapping and U-Pb dating indicate, however, that the BHA contains two volcano-sedimentary-intrusive sequences of probable opposite subduction polarity, here termed the Ammonoosuc and Quimby sequences. The Ammonoosuc sequence is defined by the Middle Ordovician Ammonoosuc Volcanics near Littleton, NH, the type area, northeast to Milan, NH, and Oquossoc, ME; it also includes black slate of the Partridge Formation ( C. bicornis--zone graptolites, ???457 Ma). Related metamorphosed intrusives are the tonalitic Joslin Turn pluton (469 ?? 2 Ma), the Cambridge Black granitic pluton (468 ?? 3 Ma), and gabbro, tonalite (467 ?? 4 Ma), and sheeted diabase of the Chickwolnepy intrusions. These intrusives cut lowermost Ammonoosuc (therefore >469 Ma). Probable uppermost Ammonoosuc is dated at 465 ?? 6 and 461 ?? 8 Ma. Successively below the Ammonoosuc are the Dead River and Hurricane Mountain Formations (flysch and melange), and the Jim Pond Formation (484 ?? 5 Ma) and Boil Mountain Complex (both ophiolite), which are structurally underlain by the Neoproterozoic(?) Chain Lakes massif. The Quimby sequence is defined by the Lower Silurian(?) to Upper Ordovician Quimby Formation, composed of bimodal volcanics (443 ?? 4 Ma) and sulfidic shale and graywacke that lie conformably to unconformably above the Ammmonoosuc Volcanics and Partridge Formation. Also in the Quimby sequence are several granitic to sparsely gabbroic plutons of the Highlandcroft (441-452 Ma) and Oliverian (435-456 Ma) Plutonic Suites, which intrude the Dead River, Ammonoouc and Partridge, but not the Quimby Formation. Based on faunal, paleolatitude, and isotopic data, the Ammonoosuc sequence and its correlatives and underlying sequences formed off the southern Laurentian margin, but northwest of the principal Iapetan suture, or Red Indian line. The Boil Mountain-Jim Pond-Hurricane Mountain sequence was ramped northwestward over the Chain Lakes massif at ???475 Ma, on the basal Boil Mountain surface. This obduction probably occurred slightly before obduction on the Baie Vert-Brompton surface (BBL), farther NW, over the Laurentian margin, and was followed by Dead River flysch sedimentation, which ended with the abrupt onset of Ammonoosuc-sequence arc magmatism at ???470 Ma. Ammonoosuc eruptions probably ended at ???460 Ma, when Iapetus closed along the Red Indian line. During a following magmatic hiatus of ???3-5 m.y., now represented by portions of the Partridge Formation that overlie the Ammonoosuc Volcanics, subduction polarity reversed, and subduction resumed below the northwest-dipping Brunswick subduction complex (BSC) of New Brunswick, Canada. Quimby-sequence magmatism (???456-435 Ma) on the newly accreted Laurentian margin occurred above the BSC, whose footwall is now buried to the southeast by mainly Silurian clastic sediments of the Merrimack-Fredericton trough, deposited in the "Fredericton Sea". In Silurian to Early Devonian time, the NW-dipping BSC footwall was paired with a SE-dipping subduction zone that produced arc magmas of the Coastal Volcanic belt, built on the composite Avalon and adjacent peri-Avalonian terranes. Orogen-normal extension produced by rapid rollback of both subduction zones narrowed the Fredericton Sea, produced the Central Maine and Connecticut Valley-Gaspe?? basins, and culminated in the Acadian orogeny when the sea completely closed in Early Devonian time. Published by Elsevier Science Ltd.

Stratigraphy, geochronology, and accretionary terrane settings of two Bronson Hill arc sequences, northern New England

NASA Astrophysics Data System (ADS)

Moench, Robert H.; Aleinikoff, John N.

The Ammonoosuc Volcanics, Partridge Formation, and the Oliverian and Highlandcroft Plutonic Suites of the Bronson Hill anticlinorium (BHA) in axial New England are widely accepted as a single Middle to Late Ordovician magmatic arc that was active during closure of Iapetus. Mapping and U-Pb dating indicate, however, that the BHA contains two volcano-sedimentary-intrusive sequences of probable opposite subduction polarity, here termed the Ammonoosuc and Quimby sequences. The Ammonoosuc sequence is defined by the Middle Ordovician Ammonoosuc Volcanics near Littleton, NH, the type area, northeast to Milan, NH, and Oquossoc, ME; it also includes black slate of the Partridge Formation ( C. bicornis--zone graptolites, ∼457 Ma). Related metamorphosed intrusives are the tonalitic Joslin Turn pluton (469 ± 2 Ma), the Cambridge Black granitic pluton (468 ± 3 Ma), and gabbro, tonalite (467 ± 4 Ma), and sheeted diabase of the Chickwolnepy intrusions. These intrusives cut lowermost Ammonoosuc (therefore >469 Ma). Probable uppermost Ammonoosuc is dated at 465 ± 6 and 461 ± 8 Ma. Successively below the Ammonoosuc are the Dead River and Hurricane Mountain Formations (flysch and melange), and the Jim Pond Formation (484 ± 5 Ma) and Boil Mountain Complex (both ophiolite), which are structurally underlain by the Neoproterozoic(?) Chain Lakes massif. The Quimby sequence is defined by the Lower Silurian(?) to Upper Ordovician Quimby Formation, composed of bimodal volcanics (443 ± 4 Ma) and sulfidic shale and graywacke that lie conformably to unconformably above the Ammmonoosuc Volcanics and Partridge Formation. Also in the Quimby sequence are several granitic to sparsely gabbroic plutons of the Highlandcroft (441-452 Ma) and Oliverian (435-456 Ma) Plutonic Suites, which intrude the Dead River, Ammonoouc and Partridge, but not the Quimby Formation. Based on faunal, paleolatitude, and isotopic data, the Ammonoosuc sequence and its correlatives and underlying sequences formed off the southern Laurentian margin, but northwest of the principal Iapetan suture, or Red Indian line. The Boil Mountain-Jim Pond-Hurricane Mountain sequence was ramped northwestward over the Chain Lakes massif at ∼475 Ma, on the basal Boil Mountain surface. This obduction probably occurred slightly before obduction on the Baie Vert-Brompton surface (BBL), farther NW, over the Laurentian margin, and was followed by Dead River flysch sedimentation, which ended with the abrupt onset of Ammonoosuc-sequence arc magmatism at ∼470 Ma. Ammonoosuc eruptions probably ended at ∼460 Ma, when Iapetus closed along the Red Indian line. During a following magmatic hiatus of ∼3-5 m.y., now represented by portions of the Partridge Formation that overlie the Ammonoosuc Volcanics, subduction polarity reversed, and subduction resumed below the northwest-dipping Brunswick subduction complex (BSC) of New Brunswick, Canada. Quimby-sequence magmatism (∼456-435 Ma) on the newly accreted Laurentian margin occurred above the BSC, whose footwall is now buried to the southeast by mainly Silurian clastic sediments of the Merrimack-Fredericton trough, deposited in the “Fredericton Sea”. In Silurian to Early Devonian time, the NW-dipping BSC footwall was paired with a SE-dipping subduction zone that produced arc magmas of the Coastal Volcanic belt, built on the composite Avalon and adjacent peri-Avalonian terranes. Orogen-normal extension produced by rapid rollback of both subduction zones narrowed the Fredericton Sea, produced the Central Maine and Connecticut Valley-Gaspé basins, and culminated in the Acadian orogeny when the sea completely closed in Early Devonian time.

Stratigraphy, geochronology, and accretionary terrane settings of two Bronson Hill arc sequences, northern New England

NASA Astrophysics Data System (ADS)

Moench, Robert H.; Aleinikoff, John N.

2002-01-01

The Ammonoosuc Volcanics, Partridge Formation, and the Oliverian and Highlandcroft Plutonic Suites of the Bronson Hill anticlinorium (BHA) in axial New England are widely accepted as a single Middle to Late Ordovician magmatic arc that was active during closure of Iapetus. Mapping and U-Pb dating indicate, however, that the BHA contains two volcano-sedimentary-intrusive sequences of probable opposite subduction polarity, here termed the Ammonoosuc and Quimby sequences. The Ammonoosuc sequence is defined by the Middle Ordovician Ammonoosuc Volcanics near Littleton, N.H., the type area, northeast to Milan, N.H., and Oquossoc, Me.; it also includes black slate of the Partidge Formation ( C. bicornis zone graptolites, ∼457 Ma). Related metamorphosed intrusive are the tonalitic Joslin Turn pluton (469±2 Ma), the Cambridge Black granitic pluton (468±3 Ma), and gabbro, tonalite (467±4 Ma), and sheeted diabase of the Chickwolnepy instructions. These intrusives cut lowermost Ammonoosuc (therefore >469 Ma). Probable uppermost Ammonoosuc is dated at 465±6 and 461±8 Ma. Successively below the Ammonoosuc are the Dead River and Hurricane Mountain Formations (flysch and melange), and the Jim Pond Formation (484±5 Ma) and Boil Mountain Complex (both ophiolite), which are structurally underlain by the Neoproterozoic(?) Chain Lakes massif. The Quimby sequence is defined by the Lower Silurian(?) to Upper Ordovician Quimby Formation, composed of bimodal volcanics (443±4 Ma) and sulfidic shale and graywacke that lie conformably to unconformably above the Ammonoosuc Volcanics and Partridge Formation. Also in the Quimby sequence are several granitic to sparsely gabbroic plutons of the Highlandcroft (441-452 Ma) and Oliverian (435-456 Ma) Plutonic Suites, which intrude the Dead River, Ammonoosuc and Partridge, but not the Quimby Formation. Based on faunal, paleolatitude, and isotropic data, the Ammonoosuc sequence and its correlative and underlying sequences formed off the southern Laurentian margin, but northwest of the principal Iapetan suture, or Red Indian line (RIL). The Boil Mountain-Jim Pond-Hurricane Mountain sequence was ramped northwestward over the Chain Lakes massif at ∼475 Ma, on the basal Boil Mountain surface. This obduction probably occurred slightly before obduction on the Baie Verte-Brompton surface (BBL), farther NW, over the Laurentian margin, and was followed by Dead River flysch sedimentation, which ended with the abrupt onset of Ammonoosuc-sequence arc magmatism at ∼470 Ma. Ammonoosuc eruptions probably ended at ∼460 Ma, when Iapetus closed along the Red Indian line. During a following magmatic hiatus of ∼3-5 m.y., now represented by portions of the Partridge Formation that overlie the Ammonoosuc Volcanics, subduction polarity reversed, and subduction resumed below the northwest-dipping Brunswick subduction complex (BSC) of New Brunswick, Canada. Quimby-sequence magmatism (∼456-435 Ma) on the the newly accreted Laurentian margin occurred above the BSC, whose footwall is now buried to the southeast by mainly Silurian clastic sediments of the Merrimack-Fredericton trough, deposited in the “Fredericton Sea”. In Silurian to Early Devonian time, the NW-dipping BSC footwall was paired with a SE-dipping subduction zone that produced arc magmas of the Coastal Volcanic belt, built on the composite Avalon and adjacent peri-Avalonian terranes. Orogen-normal extension produced by rapid rollback of both subduction zones narrowed the Fredericton Sea, produced the Central Maine and Connecticut Valley-Gaspé basins, and culminated in the Acadian orogeny when the sea completely closed in Early Devonian time.

Quality of life in Parkinson's disease: the relative importance of the symptoms.

PubMed

Rahman, Shibley; Griffin, Harry J; Quinn, Niall P; Jahanshahi, Marjan

2008-07-30

A body of literature now exists, which demonstrates that idiopathic Parkinson's disease (PD) has a major negative impact on quality of life (QoL), and that depression and cognitive impairment are among the main predictors of poor QoL in this disorder. Relatively little work has been done to assess the differential contribution of the specific symptoms of PD to QoL, which was the aim of this study. One hundred thirty patients with PD completed a booklet of questionnaires, which included the PDQ39 as a disease-specific measure of QoL, a symptom checklist, a mobility checklist, as well as patient ratings of disease stage and disability. The results indicated that the contribution of physical, medication-related, and cognitive/psychiatric symptoms to QoL can be significant. Sudden unpredictable on/off states, difficulty in dressing, difficulty in walking, falls, depression, and confusion were PD symptoms, which significantly influenced QoL scores. Among the mobility problems associated with PD, start hesitation, shuffling gait, freezing, festination, propulsion, and difficulty in turning had a significant effect on QoL scores. In addition to depression and anxiety, the major predictors of QoL were shuffling, difficulty turning, falls, difficulty in dressing, fatigue, confusion, autonomic disturbance particularly urinary incontinence, unpredictable on/off fluctuations, and sensory symptoms such as pain. The implications of these results for the medical management of PD are discussed. Copyright 2007 Movement Disorder Society.

Passive gas-gap heat switch for adiabatic demagnetization refrigerator

NASA Technical Reports Server (NTRS)

Shirron, Peter J. (Inventor); Di Pirro, Michael J. (Inventor)

2005-01-01

A passive gas-gap heat switch for use with a multi-stage continuous adiabatic demagnetization refrigerator (ADR). The passive gas-gap heat switch turns on automatically when the temperature of either side of the switch rises above a threshold value and turns off when the temperature on either side of the switch falls below this threshold value. One of the heat switches in this multistage process must be conductive in the 0.25? K to 0.3? K range. All of the heat switches must be capable of switching off in a short period of time (1-2 minutes), and when off to have a very low thermal conductance. This arrangement allows cyclic cooling cycles to be used without the need for separate heat switch controls.

Project summary

NASA Technical Reports Server (NTRS)

1991-01-01

California Polytechnic State University's design project for the 1990-91 school year was the design of a close air support aircraft. There were eight design groups that participated and were given requests for proposals. These proposals contained mission specifications, particular performance and payload requirements, as well as the main design drivers. The mission specifications called for a single pilot weighing 225 lb with equipment. The design mission profile consisted of the following: (1) warm-up, taxi, take off, and accelerate to cruise speed; (2) dash at sea level at 500 knots to a point 250 nmi from take off; (3) combat phase, requiring two combat passes at 450 knots that each consist of a 360 deg turn and an energy increase of 4000 ft. - at each pass, half of air-to-surface ordnance is released; (4) dash at sea level at 500 knots 250 nmi back to base; and (5) land with 20 min of reserve fuel. The request for proposal also specified the following performance requirements with 50 percent internal fuel and standard stores: (1) the aircraft must be able to accelerate from Mach 0.3 to 0.5 at sea level in less than 20 sec; (2) required turn rates are 4.5 sustained g at 450 knots at sea level; (3) the aircraft must have a reattack time of 25 sec or less (reattack time was defined as the time between the first and second weapon drops); (4) the aircraft is allowed a maximum take off and landing ground roll of 2000 ft. The payload requirements were 20 Mk 82 general-purpose free-fall bombs and racks; 1 GAU-8A 30-mm cannon with 1350 rounds; and 2 AIM-9L Sidewinder missiles and racks. The main design drivers expressed in the request for proposal were that the aircraft should be survivable and maintainable. It must be able to operate in remote areas with little or no maintenance. Simplicity was considered the most important factor in achieving the former goal. In addition, the aircraft must be low cost both in acquisition and operation. The summaries of the aircraft configurations developed by the eight groups are presented.

A reversible metal ion fueled DNA three-way junction molecular device for "turn-on and -off" fluorescence detection of mercury ions (II) and biothiols respectively with high selectivity and sensitivity.

PubMed

Ma, Long; Wu, Guanrong; Li, Yufeng; Qin, Ping; Meng, Lingpei; Liu, Haiyan; Li, Yuyin; Diao, Aipo

2015-11-21

We constructed a reversible molecular device in the nanoscale based on a DNA three-way junction (3WJ) fueled by Hg(2+) binding and sequestration. It is highly responsive to external stimuli, which brings about optically detectable global structural changes. Such a DNA device can serve as a novel "turn-on and -off" fluorescent sensor for Hg(2+) and biothiol detection with high selectivity and sensitivity.

Proceedings of Annual Precise Time and Time Interval (PTTI) Applications and Planning Meeting (23rd) held in Pasadena, California on December 3-5, 1991

DTIC Science & Technology

1991-12-05

second overshoot. Automatic steering was turned off for 9 days following the initial undershoot ( 48120 to 48129) and turned off from 48160 to the end...35780 0.08 A A 0.04 AA A A A35776 AA A A A AA A A A AL’ AA I- A 12 ,- U.U A __,, 0.00 . A A k 35772 I I I I I I -0.04 48000 48030 48060 48090 48120

Investigation of the Current Turn-off Characteristics of a GTO Thyristor in an Inductive Pulse Forming Network

DTIC Science & Technology

2008-05-02

conduction capacity of the discharge switch; the discharge switch was a TRIAC (Littlefuse – Q6015L5) rated to block 600Vand conduct 15A. (For this circuit ...part of the test circuit to verify was the capacitor dump circuit . The capacitor bank was charged up to 200V and the TRIAC (S2 in Figure 17) was...be turned off by a GTO thyristor. During the course of the project, a series of GTO thyristors were used in an inductive pulse forming circuit to

Aptamer-based turn-on fluorescent four-branched quaternary ammonium pyrazine probe for selective thrombin detection.

PubMed

Yan, Shengyong; Huang, Rong; Zhou, Yangyang; Zhang, Ming; Deng, Minggang; Wang, Xiaolin; Weng, Xiaocheng; Zhou, Xiang

2011-01-28

In this thrombin detection system, the bright fluorescence of TASPI is almost eliminated by the DNA aptamer TBA (turn-off); however, in the presence of thrombin, it specifically binds to TBA by folding unrestricted TBA into an anti-parallel G-quadruplex structure and then releasing TASPI molecules, resulting in vivid and facile fluorescence recovery (turn-on).

Private Turns: A Student's Off-Screen Behaviors during Synchronous Online Japanese Instruction

ERIC Educational Resources Information Center

Suzuki, Satomi

2013-01-01

Although distance language education has been widely adopted in university learning, very few researchers to date have looked at off-screen behaviors of second/foreign language learners in their physical environments while they engage in synchronous (real-time) online courses. This study examined one focal student's off-screen behaviors while…

Establishment of a real-time PCR method for quantification of geosmin-producing Streptomyces spp. in recirculating aquaculture systems.

PubMed

Auffret, Marc; Pilote, Alexandre; Proulx, Emilie; Proulx, Daniel; Vandenberg, Grant; Villemur, Richard

2011-12-15

Geosmin and 2-methylisoborneol (MIB) have been associated with off-flavour problems in fish and seafood products, generating a strong negative impact for aquaculture industries. Although most of the producers of geosmin and MIB have been identified as Streptomyces species or cyanobacteria, Streptomyces spp. are thought to be responsible for the synthesis of these compounds in indoor recirculating aquaculture systems (RAS). The detection of genes involved in the synthesis of geosmin and MIB can be a relevant indicator of the beginning of off-flavour events in RAS. Here, we report a real-time polymerase chain reaction (qPCR) protocol targeting geoA sequences that encode a germacradienol synthase involved in geosmin synthesis. New geoA-related sequences were retrieved from eleven geosmin-producing Actinomycete strains, among them two Streptomyces strains isolated from two RAS. Combined with geoA-related sequences available in gene databases, we designed primers and standards suitable for qPCR assays targeting mainly Streptomyces geoA. Using our qPCR protocol, we succeeded in measuring the level of geoA copies in sand filter and biofilters in two RAS. This study is the first to apply qPCR assays to detect and quantify the geosmin synthesis gene (geoA) in RAS. Quantification of geoA in RAS could permit the monitoring of the level of geosmin producers prior to the occurrence of geosmin production. This information will be most valuable for fish producers to manage further development of off-flavour events. Copyright © 2011 Elsevier Ltd. All rights reserved.

Modelling the 2013 North Aegean (Greece) seismic sequence: geometrical and frictional constraints, and aftershock probabilities

NASA Astrophysics Data System (ADS)

Karakostas, Vassilis; Papadimitriou, Eleftheria; Gospodinov, Dragomir

2014-04-01

The 2013 January 8 Mw 5.8 North Aegean earthquake sequence took place on one of the ENE-WSW trending parallel dextral strike slip fault branches in this area, in the continuation of 1968 large (M = 7.5) rupture. The source mechanism of the main event indicates predominantly strike slip faulting in agreement with what is expected from regional seismotectonics. It was the largest event to have occurred in the area since the establishment of the Hellenic Unified Seismological Network (HUSN), with an adequate number of stations in close distances and full azimuthal coverage, thus providing the chance of an exhaustive analysis of its aftershock sequence. The main shock was followed by a handful of aftershocks with M ≥ 4.0 and tens with M ≥ 3.0. Relocation was performed by using the recordings from HUSN and a proper crustal model for the area, along with time corrections in each station relative to the model used. Investigation of the spatial and temporal behaviour of seismicity revealed possible triggering of adjacent fault segments. Theoretical static stress changes from the main shock give a preliminary explanation for the aftershock distribution aside from the main rupture. The off-fault seismicity is perfectly explained if μ > 0.5 and B = 0.0, evidencing high fault friction. In an attempt to forecast occurrence probabilities of the strong events (Mw ≥ 5.0), estimations were performed following the Restricted Epidemic Type Aftershock Sequence (RETAS) model. The identified best-fitting MOF model was used to execute 1-d forecasts for such aftershocks and follow the probability evolution in time during the sequence. Forecasting was also implemented on the base of a temporal model of aftershock occurrence, different from the modified Omori formula (the ETAS model), which resulted in probability gain (though small) in strong aftershock forecasting for the beginning of the sequence.

A novel ultrasensitive carboxymethyl chitosan-quantum dot-based fluorescence "turn on-off" nanosensor for lysozyme detection.

PubMed

Song, Yu; Li, Yang; Liu, Ziping; Liu, Linlin; Wang, Xinyan; Su, Xingguang; Ma, Qiang

2014-11-15

In this work, we developed an ultrasensitive "turn on-off" fluorescence nanosensor for lysozyme (Lyz) detection. The novel nanosensor was constructed with the carboxymethyl chitosan modified CdTe quantum dots (CMCS-QDs). Firstly, the CMCS-QDs were fabricated via the electrostatic interaction between amino groups in CMCS polymeric chains and carboxyl groups on the surface of QDs. In the fluorescence "turn-on" step, the strong binding ability between Zn(2+) and CMCS on the surface of QDs can enhance the photoluminescence intensity (PL) of QDs. In the following fluorescence "turn-off" step, the N-acetyl-glucosamine (NAG) section along the CMCS chains was hydrolyzed by Lyz. As a result, Zn(2+) was released from the surface of QDs, and the Lyz-QDs complexes were formed to quench the QDs PL. Under the optimal conditions, there was a good linear relationship between the PL of QDs and the Lyz concentration (0.1-1.2 ng/mL) with the detection limit of 0.031 ng/mL. The developed method was ultrasensitive, highly selective and fast. It has been successfully employed in the detection of Lyz in the serum with satisfactory results. Copyright © 2014 Elsevier B.V. All rights reserved.

Proline Restricts Loop I Conformation of the High Affinity WW Domain from Human Nedd4-1 to a Ligand Binding-Competent Type I β-Turn.

PubMed

Schulte, Marianne; Panwalkar, Vineet; Freischem, Stefan; Willbold, Dieter; Dingley, Andrew J

2018-04-19

Sequence alignment of the four WW domains from human Nedd4-1 (neuronal precursor cell expressed developmentally down-regulated gene 4-1) reveals that the highest sequence diversity exists in loop I. Three residues in this type I β-turn interact with the PPxY motif of the human epithelial Na + channel (hENaC) subunits, indicating that peptide affinity is defined by the loop I sequence. The third WW domain (WW3*) has the highest ligand affinity and unlike the other three hNedd4-1 WW domains or other WW domains studied contains the highly statistically preferred proline at the ( i + 1) position found in β-turns. In this report, molecular dynamics simulations and experimental data were combined to characterize loop I stability and dynamics. Exchange of the proline to the equivalent residue in WW4 (Thr) results in the presence of a predominantly open seven residue Ω loop rather than the type I β-turn conformation for the wild-type apo-WW3*. In the presence of the ligand, the structure of the mutated loop I is locked into a type I β-turn. Thus, proline in loop I ensures a stable peptide binding-competent β-turn conformation, indicating that amino acid sequence modulates local flexibility to tune binding preferences and stability of dynamic interaction motifs.

Cutting the Cord-2

NASA Technical Reports Server (NTRS)

2004-01-01

This animation shows the view from the rear hazard avoidance cameras on the Mars Exploration Rover Spirit as the rover turns 45 degrees clockwise. This maneuver is the first step in a 3-point turn that will rotate the rover 115 degrees to face west. The rover must make this turn before rolling off the lander because airbags are blocking it from exiting from the front lander petal. Before this crucial turn took place, engineers instructed the rover to cut the final cord linking it to the lander. The turn took around 30 minutes to complete.

A demonstration device for cosmic rays telescopes

NASA Astrophysics Data System (ADS)

Esposito, Salvatore

2018-01-01

We describe a hands-on accurate demonstrator for cosmic rays realized by six high school students. The main aim is to show the relevance and the functioning of the principal parts of a cosmic ray telescope (muon detector), with the help of two large sized wooden artefacts. The first one points out how cosmic rays can be tracked in a muon telescope, while the other one shows the key avalanche process of electronic ionization that effectively allows muon detection through a photomultiplier. Incoming cosmic rays are visualized in terms of laser beams, whose 3D trajectory is highlighted by turning on LEDs on two orthogonal matrices. Instead the avalanche ionization process is demonstrated through the avalanche falling off glass marbles on an inclined plane, finally turning on a LED. A pictured poster accompanying the demonstrator is as effective in assisting cosmic ray demonstration and its detection. The success of the demonstrator has been fully proven by the general public during a science festival, in which the corresponding project won the Honorable Mention in a dedicated competition.

Using the CIFIST grid of CO5BOLD 3D model atmospheres to study the effects of stellar granulation on photometric colours. II. The role of convection across the H-R diagram

NASA Astrophysics Data System (ADS)

Kučinskas, A.; Klevas, J.; Ludwig, H.-G.; Bonifacio, P.; Steffen, M.; Caffau, E.

2018-05-01

Aims: We studied the influence of convection on the spectral energy distributions (SEDs), photometric magnitudes, and colour indices of different types of stars across the H-R diagram. Methods: The 3D hydrodynamical CO5BOLD, averaged ⟨3D⟩, and 1D hydrostatic LHD model atmospheres were used to compute SEDs of stars on the main sequence (MS), main sequence turn-off (TO), subgiant branch (SGB), and red giant branch (RGB), in each case at two different effective temperatures and two metallicities, [M/H] = 0.0 and - 2.0. Using the obtained SEDs, we calculated photometric magnitudes and colour indices in the broad-band Johnson-Cousins UBVRI and 2MASS JHKs, and the medium-band Strömgren uvby photometric systems. Results: The 3D-1D differences in photometric magnitudes and colour indices are small in both photometric systems and typically do not exceed ± 0.03 mag. Only in the case of the coolest giants located on the upper RGB are the differences in the U and u bands able reach ≈-0.2 mag at [M/H] = 0.0 and ≈-0.1 mag at [M/H] = -2.0. Generally, the 3D-1D differences are largest in the blue-UV part of the spectrum and decrease towards longer wavelengths. They are also sensitive to the effective temperature and are significantly smaller in hotter stars. Metallicity also plays a role and leads to slightly larger 3D-1D differences at [M/H] = 0.0. All these patterns are caused by a complex interplay between the radiation field, opacities, and horizontal temperature fluctuations that occur due to convective motions in stellar atmospheres. Although small, the 3D-1D differences in the magnitudes and colour indices are nevertheless comparable to or larger than typical photometric uncertainties and may therefore cause non-negligible systematic differences in the estimated effective temperatures.

Species of Philometra (Nematoda, Philometridae) from fishes off the Mediterranean coast of Africa, with a description of Philometra rara n. sp. from Hyporthodus haifensis and a molecular analysis of Philometra saltatrix from Pomatomus saltatrix.

PubMed

Moravec, František; Chaabane, Amira; Neifar, Lassad; Gey, Delphine; Justine, Jean-Lou

2017-01-01

Two gonad-infecting species of Philometra Costa, 1845 (Nematoda, Philometridae) were recorded for the first time from marine perciform fishes off Tunisia and Libya: Philometra rara n. sp. from the rare, deep-water Haifa grouper Hyporthodus haifensis (Serranidae) off Libya and Philometra saltatrix Ramachandran, 1973 from the bluefish Pomatomus saltatrix (Pomatomidae) off Tunisia. Identification of both fish species was confirmed by molecular barcoding. Light and scanning electron microscope studies of Ph. rara n. sp. showed that it is characterized by the length of spicules (216-219 μm) and the gubernaculum (90-93 μm), the gubernaculum/spicules length ratio (1:2.32-2.43), and mainly by the shape and structure of the distal end of the gubernaculum (shovel-shaped with a wide median smooth field in dorsal view), appearing as having a dorsal protuberance in lateral view, and by the structure of the male caudal mound (dorsally interrupted); large subgravid females (70-137 mm long) are characterized by the presence of four oval submedian cephalic elevations, each of them bearing a pair of cephalic papillae of the outer circle. The finding of Ph. saltatrix off Tunisia confirms that this species is widespread throughout the Mediterranean region. A molecular analysis of our Ph. saltatrix specimens and other available philometrid cytochrome c oxidase 1 (COI) sequences showed that most species have robust clades. Sequences of Ph. saltatrix from Tunisia diverge from Ph. saltatrix from Brazil and the USA, suggesting that speciation is currently occurring between populations from both sides of the Atlantic Ocean. © F. Moravec et al., published by EDP Sciences, 2017.

Species of Philometra (Nematoda, Philometridae) from fishes off the Mediterranean coast of Africa, with a description of Philometra rara n. sp. from Hyporthodus haifensis and a molecular analysis of Philometra saltatrix from Pomatomus saltatrix

PubMed Central

2017-01-01

Two gonad-infecting species of Philometra Costa, 1845 (Nematoda, Philometridae) were recorded for the first time from marine perciform fishes off Tunisia and Libya: Philometra rara n. sp. from the rare, deep-water Haifa grouper Hyporthodus haifensis (Serranidae) off Libya and Philometra saltatrix Ramachandran, 1973 from the bluefish Pomatomus saltatrix (Pomatomidae) off Tunisia. Identification of both fish species was confirmed by molecular barcoding. Light and scanning electron microscope studies of Ph. rara n. sp. showed that it is characterized by the length of spicules (216–219 μm) and the gubernaculum (90–93 μm), the gubernaculum/spicules length ratio (1:2.32–2.43), and mainly by the shape and structure of the distal end of the gubernaculum (shovel-shaped with a wide median smooth field in dorsal view), appearing as having a dorsal protuberance in lateral view, and by the structure of the male caudal mound (dorsally interrupted); large subgravid females (70–137 mm long) are characterized by the presence of four oval submedian cephalic elevations, each of them bearing a pair of cephalic papillae of the outer circle. The finding of Ph. saltatrix off Tunisia confirms that this species is widespread throughout the Mediterranean region. A molecular analysis of our Ph. saltatrix specimens and other available philometrid cytochrome c oxidase 1 (COI) sequences showed that most species have robust clades. Sequences of Ph. saltatrix from Tunisia diverge from Ph. saltatrix from Brazil and the USA, suggesting that speciation is currently occurring between populations from both sides of the Atlantic Ocean. PMID:28287390

Ultrafast Power Processor for Smart Grid Power Module Development

DOE Office of Scientific and Technical Information (OSTI.GOV)

MAITRA, ARINDAM; LITWIN, RAY; lai, Jason

This project’s goal was to increase the switching speed and decrease the losses of the power semiconductor devices and power switch modules necessary to enable Smart Grid energy flow and control equipment such as the Ultra-Fast Power Processor. The primary focus of this project involves exploiting the new silicon-based Super-GTO (SGTO) technology and build on prototype modules already being developed. The prototype super gate-turn-off thyristor (SGTO) has been tested fully under continuously conducting and double-pulse hard-switching conditions for conduction and switching characteristics evaluation. The conduction voltage drop measurement results indicate that SGTO has excellent conduction characteristics despite inconsistency among somemore » prototype devices. Tests were conducted with two conditions: (1) fixed gate voltage and varying anode current condition, and (2) fixed anode current and varying gate voltage condition. The conduction voltage drop is relatively a constant under different gate voltage condition. In terms of voltage drop as a function of the load current, there is a fixed voltage drop about 0.5V under zero current condition, and then the voltage drop is linearly increased with the current. For a 5-kV voltage blocking device that may operate under 2.5-kV condition, the projected voltage drop is less than 2.5 V under 50-A condition, or 0.1%. If the device is adopted in a converter operating under soft-switching condition, then the converter can achieve an ultrahigh efficiency, typically above 99%. The two-pulse switching test results indicate that SGTO switching speed is very fast. The switching loss is relatively low as compared to that of the insulated-gate-bipolar-transistors (IGBTs). A special phenomenon needs to be noted is such a fast switching speed for the high-voltage switching tends to create an unexpected Cdv/dt current, which reduces the turn-on loss because the dv/dt is negative and increases the turn-off loss because the dv/dt is positive. As a result, the turn-on loss at low current is quite low, and the turn-off loss at low current is relatively high. The phenomenon was verified with junction capacitance measurement along with the dv/dt calculation. Under 2-kV test condition, the turn-on and turn-off losses at 25-A is about 3 and 9 mJ, respectively. As compared to a 4.5-kV, 60-A rated IGBT, which has turn-on and turn-off losses about 25 and 20 mJ under similar test condition, the SGTO shows significant switching loss reduction. The switching loss depends on the switching frequency, but under hard-switching condition, the SGTO is favored to the IGBT device. The only concern is during low current turn-on condition, there is a voltage bump that can translate to significant power loss and associated heat. The reason for such a current bump is not known from this study. It is necessary that the device manufacturer perform though test and provide the answer so the user can properly apply SGTO in pulse-width-modulated (PWM) converter and inverter applications.« less

Role of different β-turns in β-hairpin conformation and stability studied by optical spectroscopy.

PubMed

Wu, Ling; McElheny, Dan; Setnicka, Vladimír; Hilario, Jovencio; Keiderling, Timothy A

2012-01-01

Model β-hairpin peptides based on variations in the turn sequence of Cochran's tryptophan zipper peptide, SWTWENGKWTWK, were studied using electronic circular dichroism (ECD), fluorescence, and infrared (IR) spectroscopies. The trpzip2 Asn-Gly turn sequence was substituted with Thr-Gly, Aib-Gly, (D)Pro-Gly, and Gly-Asn (trpzip1) to study the impact of turn stability on β-hairpin formation. Stability and conformational changes of these hairpins were monitored by thermodynamic analyses of the temperature variation of both FTIR (amide I') and ECD spectral intensities. These changes were fit to a two-state model which yielded different T(m) values, representing the folding/unfolding process, for hairpins with different β-turns. Different β-turns show systematic contributions to hairpin structure formation, and their inclusion in hairpin design can modify the folding pathways. Aib-Gly or (D)Pro-Gly sequences stabilize the turn resulting in residual Trp-Trp interaction at high temperatures, but at the same time the β-structure (cross strand H-bonds) can become less stable due to constraints of the turn, as seen for (D)Pro-Gly. The structure of the Aib-Gly turn containing hairpin was determined by NMR and was shown to be like trpzip2 (Asn-Gly turn) as regards turn and strand geometries, but to differ from trpzip1 (Gly-Asn turn). The Munoz and Eaton statistical mechanically derived multistate model, tested as an alternate point of view, represented contributions from H-bonds and hydrophobic interactions as well as conformational change as interdependent. Use of different spectral methods that vary in dependence on these physical interactions along with the structural variations provided insight to the complex folding pathways of these small, well-folded peptides. Copyright © 2011 Wiley Periodicals, Inc.

Self-monitoring of driving speed.

PubMed

Etzioni, Shelly; Erev, Ido; Ishaq, Robert; Elias, Wafa; Shiftan, Yoram

2017-09-01

In-vehicle data recorders (IVDR) have been found to facilitate safe driving and are highly valuable in accident analysis. Nevertheless, it is not easy to convince drivers to use them. Part of the difficulty is related to the "Big Brother" concern: installing IVDR impairs the drivers' privacy. The "Big Brother" concern can be mitigated by adding a turn-off switch to the IVDR. However, this addition comes at the expense of increasing speed variability between drivers, which is known to impair safety. The current experimental study examines the significance of this negative effect of a turn-off switch under two experimental settings representing different incentive structures: small and large fines for speeding. 199 students were asked to participate in a computerized speeding dilemma task, where they could control the speed of their "car" using "brake" and "speed" buttons, corresponding to automatic car foot pedals. The participants in two experimental conditions had IVDR installed in their "cars", and were told that they could turn it off at any time. Driving with active IVDR implied some probability of "fines" for speeding, and the two experimental groups differed with respect to the fine's magnitude, small or large. The results indicate that the option to use IVDR reduced speeding and speed variance. In addition, the results indicate that the reduction of speed variability was maximal in the small fine group. These results suggest that using IVDR with gentle fines and with a turn-off option maintains the positive effect of IVDR, addresses the "Big Brother" concern, and does not increase speed variance. Copyright © 2017 Elsevier Ltd. All rights reserved.

Some statistical features of the aftershock temporal behavior after the M7.4 Izmit earthquake of august 17, 1999 in Turkey

NASA Astrophysics Data System (ADS)

Gospodinov, D.; Fajtin, H.; Rangelov, B.; Marekova, E.

2009-04-01

An earthquake of magnitude Mw=7.4 struck 8 km. southeast of Izmit, Turkey at 3:02 AM local time on August 17, 1999. The earthquake occurred on one of the world's longest and best studied strike-slip (horizontal motion) faults - the east-west trending North Anatolian fault. Seismologists are not able to predict the timing and sizes of individual aftershocks but stochastic modeling allows determinationof probabilities for aftershocks and larger mainshocks duringintervals following the mainshock. The most widely applied stochastic model to depict aftershocks temporal distribution is the non- homogenous Poisson process with a decaying intensity, which follows the Modified Omori Formula (MOF) (Utsu, 1961). A more complex model, considering the triggering potential of each aftershock was developed by Ogata (1988) and it was named Epidemic Type Aftershock Sequence (ETAS) model. Gospodinov and Rotondi (2006) elaborated a Restricted Epidemic Type Aftershock Sequence (RETAS) model. The latter follows the general idea that only aftershocks stronger than some cut-off magnitude possess the capability to induce secondary aftershock activity. In this work we shall consider the Restricted Epidemic Type Aftershock Sequence (RETAS) model, for which the conditional intensity function turns out to be ‘ K0eα(Mi-M0)- λ (t|Ht) = + (t- ti + c)p ti < t Mi ≥ Mth (1) Here the summation occurs for all aftershocks with magnitude bigger than or equal to Mth, which took place before time. Leaving Mth to take all possible values, one can examine all RETAS model versions between the MOF and the ETAS model on the basis of the Akaike Information Criterion AIC (Akaike, 1974) AIC = - 2max log L+ 2k (2) where k is the number of parameters used in the model and logL is the logarithm of the likelihood function. Then for the model providing the best fit, we choose the one with the smallest AIC value. The purpose of this paper is to verify versions of the RETAS model (including the MOF and the ETAS model) for the analysis of the aftershock sequence after the Mw=7.4 Izmit earthquake. The obtained results revealed that the best fit model is ETAS, for which the triggering magnitude coincides with the lower cut-off. In this case each event in the sequence can generate its own secondary events. The analysis points out that about 12 days before the Duzce earthquake a relative quiescence begins which then turns to a relative activization about 5 days before the strong shock. This can be explained by a possible realization of several stronger aftershocks before the Duzce event, which are considered as its foreshocks. Another result which can be outlined is that the MOF model is not good for a sequence in which we have secondary activity (strong aftershock or several main events). For a complex aftershock sequence as is the case for the Izmit-Duzce zone, it is adequate to apply the RETAS model which gives more possibilities to model the aftershock activity in time. This allows to perform a better geotectonic interpretation later. The analysis of the aftershock activity after the strong Izmit earthquake, executed through the RETAS model makes it possible to draw some conclusions when interpreting the results: - The RETAS model supplies a methodology to identify the best fit model of the relaxation temporal development and in our case this is the ETAS model (each aftershock is capable to trigger secondary activity). - The recognized best fit model for the sequence provides an opportunity to identify separate periods of relative quiescence and activization. A quiescence period of several days was identified before the strongest aftershock - the Duzce earthquake. - For standard aftershock sequences the different versions of the RETAS model (ETAS, MOF and the intermediate ones) are closer to each other. For a more complex sequence with strong aftershock activity the RETAS model provides better possibilities to model the temporal aspect of the relaxation process and to perform the most adequate geotectonic interpretation. References Akaike, H., 1974, A new look at the statistical model identification. IEEE Trans. Automat. Contr. AC-19, 716-723. Gospodinov, D., R. Rotondi, 2006, Statistical Analysis of Triggered Seismicity in the Kresna Region of SW Bulgaria (1904) and the Umbria-Marche Region of Central Italy (1997). Pure Appl. Geophys. 163, 1597-1615. Ogata, Y., 1988, Statistical models for earthquake occurrences and residual analysis for point processes. J. Am. Stat. Assoc. 83, 9-27. Utsu, T., 1961, A statistical study on the occurrence of aftershocks, Geophys. Mag., 30, 521-605

Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

DOE Office of Scientific and Technical Information (OSTI.GOV)

Czarnecki, Olaf; Bryan, Anthony C.; Jawdy, Sara S.

Genetic engineering of plants that results in successful establishment of new biochemical or regulatory pathways requires stable introduction of one or more genes into the plant genome. It might also be necessary to down-regulate or turn off expression of endogenous genes in order to reduce activity of competing pathways. An established way to knockdown gene expression in plants is expressing a hairpin-RNAi construct, eventually leading to degradation of a specifically targeted mRNA. Knockdown of multiple genes that do not share homologous sequences is still challenging and involves either sophisticated cloning strategies to create vectors with different serial expression constructs ormore » multiple transformation events that is often restricted by a lack of available transformation markers. Synthetic RNAi fragments were assembled in yeast carrying homologous sequences to six or seven non-family genes and introduced into pAGRIKOLA. Transformation of Arabidopsis thaliana and subsequent expression analysis of targeted genes proved efficient knockdown of all target genes. In conclusion, we present a simple and cost-effective method to create constructs to simultaneously knockdown multiple non-family genes or genes that do not share sequence homology. The presented method can be applied in plant and animal synthetic biology as well as traditional plant and animal genetic engineering.« less

Adeno-associated virus inverted terminal repeats stimulate gene editing.

PubMed

Hirsch, M L

2015-02-01

Advancements in genome editing have relied on technologies to specifically damage DNA which, in turn, stimulates DNA repair including homologous recombination (HR). As off-target concerns complicate the therapeutic translation of site-specific DNA endonucleases, an alternative strategy to stimulate gene editing based on fragile DNA was investigated. To do this, an episomal gene-editing reporter was generated by a disruptive insertion of the adeno-associated virus (AAV) inverted terminal repeat (ITR) into the egfp gene. Compared with a non-structured DNA control sequence, the ITR induced DNA damage as evidenced by increased gamma-H2AX and Mre11 foci formation. As local DNA damage stimulates HR, ITR-mediated gene editing was investigated using DNA oligonucleotides as repair substrates. The AAV ITR stimulated gene editing >1000-fold in a replication-independent manner and was not biased by the polarity of the repair oligonucleotide. Analysis of additional human DNA sequences demonstrated stimulation of gene editing to varying degrees. In particular, inverted yet not direct, Alu repeats induced gene editing, suggesting a role for DNA structure in the repair event. Collectively, the results demonstrate that inverted DNA repeats stimulate gene editing via double-strand break repair in an episomal context and allude to efficient gene editing of the human chromosome using fragile DNA sequences.

Simultaneous knockdown of six non-family genes using a single synthetic RNAi fragment in Arabidopsis thaliana

DOE PAGES

Czarnecki, Olaf; Bryan, Anthony C.; Jawdy, Sara S.; ...

2016-02-17

Genetic engineering of plants that results in successful establishment of new biochemical or regulatory pathways requires stable introduction of one or more genes into the plant genome. It might also be necessary to down-regulate or turn off expression of endogenous genes in order to reduce activity of competing pathways. An established way to knockdown gene expression in plants is expressing a hairpin-RNAi construct, eventually leading to degradation of a specifically targeted mRNA. Knockdown of multiple genes that do not share homologous sequences is still challenging and involves either sophisticated cloning strategies to create vectors with different serial expression constructs ormore » multiple transformation events that is often restricted by a lack of available transformation markers. Synthetic RNAi fragments were assembled in yeast carrying homologous sequences to six or seven non-family genes and introduced into pAGRIKOLA. Transformation of Arabidopsis thaliana and subsequent expression analysis of targeted genes proved efficient knockdown of all target genes. In conclusion, we present a simple and cost-effective method to create constructs to simultaneously knockdown multiple non-family genes or genes that do not share sequence homology. The presented method can be applied in plant and animal synthetic biology as well as traditional plant and animal genetic engineering.« less

Prediction of beta-turns from amino acid sequences using the residue-coupled model.

PubMed

Guruprasad, K; Shukla, S

2003-04-01

We evaluated the prediction of beta-turns from amino acid sequences using the residue-coupled model with an enlarged representative protein data set selected from the Protein Data Bank. Our results show that the probability values derived from a data set comprising 425 protein chains yielded an overall beta-turn prediction accuracy 68.74%, compared with 94.7% reported earlier on a data set of 30 proteins using the same method. However, we noted that the overall beta-turn prediction accuracy using probability values derived from the 30-protein data set reduces to 40.74% when tested on the data set comprising 425 protein chains. In contrast, using probability values derived from the 425 data set used in this analysis, the overall beta-turn prediction accuracy yielded consistent results when tested on either the 30-protein data set (64.62%) used earlier or a more recent representative data set comprising 619 protein chains (64.66%) or on a jackknife data set comprising 476 representative protein chains (63.38%). We therefore recommend the use of probability values derived from the 425 representative protein chains data set reported here, which gives more realistic and consistent predictions of beta-turns from amino acid sequences.

Site-Specific Dynamics of β-Sheet Peptides with (D) Pro-Gly Turns Probed by Laser-Excited Temperature-Jump Infrared Spectroscopy.

PubMed

Popp, Alexander; Scheerer, David; Chi, Heng; Keiderling, Timothy A; Hauser, Karin

2016-05-04

Turn residues and side-chain interactions play an important role for the folding of β-sheets. We investigated the conformational dynamics of a three-stranded β-sheet peptide ((D) P(D) P) and a two-stranded β-hairpin (WVYY-(D) P) by time-resolved temperature-jump (T-jump) infrared spectroscopy. Both peptide sequences contain (D) Pro-Gly residues that favor a tight β-turn. The three-stranded β-sheet (Ac-VFITS(D) PGKTYTEV(D) PGOKILQ-NH2 ) is stabilized by the turn sequences, whereas the β-hairpin (SWTVE(D) PGKYTYK-NH2 ) folding is assisted by both the turn sequence and hydrophobic cross-strand interactions. Relaxation times after the T-jump were monitored as a function of temperature and occur on a sub-microsecond time scale, (D) P(D) P being faster than WVYY-(D) P. The Xxx-(D) Pro tertiary amide provides a detectable IR band, allowing us to probe the dynamics site-specifically. The relative importance of the turn versus the intrastrand stability in β-sheet formation is discussed. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Theory for controlling individual self-propelled micro-swimmers by photon nudging I: directed transport.

PubMed

Selmke, Markus; Khadka, Utsab; Bregulla, Andreas P; Cichos, Frank; Yang, Haw

2018-04-18

Photon nudging is a new experimental method which enables the force-free manipulation and localization of individual self-propelled artificial micro-swimmers in fluidic environments. It uses a weak laser to stochastically and adaptively turn on and off the swimmer's propulsion when the swimmer, through rotational diffusion, points towards or away from its target, respectively. This contribution presents a theoretical framework for the statistics of both 2D and 3D controls. The main results are: the on- and off-time distributions for the controlling laser, the arrival time statistics for the swimmer to reach a remote target, and how the experimentally accessible control parameters influence the control, e.g., the optimal acceptance angle for directed transport. The results are general in that they are independent of the propulsion or the actuation mechanisms. They provide a concrete physical picture for how a single artificial micro-swimmer could be navigated under thermal fluctuations-insights that could also be useful for understanding biological micro-swimmers.

Global excitation of wave phenomena in a dissipative multiconstituent medium. I - Transfer function of the earth's thermosphere. II - Impulsive perturbations in the earth's thermosphere

NASA Technical Reports Server (NTRS)

Mayr, H. G.; Harris, I.; Herrero, F. A.; Varosi, F.

1984-01-01

A transfer function approach is taken in constructing a spectral model of the acoustic-gravity wave response in a multiconstituent thermosphere. The model is then applied to describing the thermospheric response to various sources around the globe. Zonal spherical harmonics serve to model the horizontal variations in propagating waves which, when integrated with respect to height, generate a transfer function for a vertical source distribution in the thermosphere. Four wave components are characterized as resonance phenomena and are associated with magnetic activity and ionospheric disturbances. The waves are either trapped or propagate, the latter becoming significant when possessing frequencies above 3 cycles/day. The energy input is distributed by thermospheric winds. The disturbances decay slowly, mainly due to heat conduction and diffusion. Gravity waves appear abruptly and are connected to a sudden switching on or off of a source. Turn off of a source coincides with a reversal of the local atmospheric circulation.

Method for high resolution magnetic resonance analysis using magic angle technique

DOEpatents

Wind, Robert A.; Hu, Jian Zhi

2003-12-30

A method of performing a magnetic resonance analysis of a biological object that includes placing the object in a main magnetic field (that has a static field direction) and in a radio frequency field; rotating the object at a frequency of less than about 100 Hz around an axis positioned at an angle of about 54.degree.44' relative to the main magnetic static field direction; pulsing the radio frequency to provide a sequence that includes a phase-corrected magic angle turning pulse segment; and collecting data generated by the pulsed radio frequency. The object may be reoriented about the magic angle axis between three predetermined positions that are related to each other by 120.degree.. The main magnetic field may be rotated mechanically or electronically. Methods for magnetic resonance imaging of the object are also described.

Method for high resolution magnetic resonance analysis using magic angle technique

DOEpatents

Wind, Robert A.; Hu, Jian Zhi

2004-12-28

A method of performing a magnetic resonance analysis of a biological object that includes placing the object in a main magnetic field (that has a static field direction) and in a radio frequency field; rotating the object at a frequency of less than about 100 Hz around an axis positioned at an angle of about 54.degree.44' relative to the main magnetic static field direction; pulsing the radio frequency to provide a sequence that includes a phase-corrected magic angle turning pulse segment; and collecting data generated by the pulsed radio frequency. The object may be reoriented about the magic angle axis between three predetermined positions that are related to each other by 120.degree.. The main magnetic field may be rotated mechanically or electronically. Methods for magnetic resonance imaging of the object are also described.

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

PubMed Central

Naito, Yuki; Hino, Kimihiro; Bono, Hidemasa; Ui-Tei, Kumiko

2015-01-01

Summary: CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a ∼20 nt targeted sequence. The target sequence requirements are twofold. First, the 5′-NGG protospacer adjacent motif (PAM) sequence must be located adjacent to the target sequence. Second, the target sequence should be specific within the entire genome in order to avoid off-target editing. CRISPRdirect enables users to easily select rational target sequences with minimized off-target sites by performing exhaustive searches against genomic sequences. The server currently incorporates the genomic sequences of human, mouse, rat, marmoset, pig, chicken, frog, zebrafish, Ciona, fruit fly, silkworm, Caenorhabditis elegans, Arabidopsis, rice, Sorghum and budding yeast. Availability: Freely available at http://crispr.dbcls.jp/. Contact: y-naito@dbcls.rois.ac.jp Supplementary information: Supplementary data are available at Bioinformatics online. PMID:25414360

Leakage Account for Radial Face Contact Seal in Aircraft Engine Support

NASA Astrophysics Data System (ADS)

Vinogradov, A. S.; Sergeeva, T. V.

2018-01-01

The article is dedicated to the development of a methodology for the radial face contact seal design taking into consideration the supporting elements deformations in different aircraft engine operating modes. Radial face contact seals are popular in the aircraft engines bearing support. However, there are no published leakage calculation methodologies of these seals. Radial face contact seal leakage is determined by the gap clearance in the carbon seal ring split. In turn, the size gap clearance depends on the deformation of the seal assembly parts and from the engine operation. The article shows the leakage detection sequence in the intershaft radial face contact seal of the compressor support for take-off and cruising modes. Evaluated calculated leakage values (2.4 g/s at takeoff and 0.75 g/s at cruising) go with experience in designing seals.

Legionnaires' Disease Outbreak at a Long-Term Care Facility Caused by a Cooling Tower Using an Automated Disinfection System--Ohio, 2013.

PubMed

Quinn, Celia; Demirjian, Alicia; Watkins, Louise Francois; Tomczyk, Sara; Lucas, Claressa; Brown, Ellen; Kozak-Muiznieks, Natalia; Benitez, Alvaro; Garrison, Laurel E; Kunz, Jasen; Brewer, Scott; Eitniear, Samantha; DiOrio, Mary

2015-12-01

On July 9, 2013, an outbreak of Legionnaires' disease (LD) was identified at Long-Term Care Facility A in central Ohio. This article describes the investigation of the outbreak and identification of the outbreak source, a cooling tower using an automated biocide delivery system. In total, 39 outbreak LD cases were identified; among these, six patients died. Water samples from a cooling tower were positive for Legionella pneumophila serogroup 1, reactive to monoclonal antibody 2, with matching sequence type to a patient isolate. An electronic control system turned off cooling tower pumps during low-demand periods, preventing delivery of disinfectant by a timed-release system, and leading to amplification of Legionella in the cooling tower. Guidelines for tower maintenance should address optimal disinfection when using automated systems.

Blood pressure measurement and display system

NASA Technical Reports Server (NTRS)

Farkas, A. J.

1972-01-01

System is described that employs solid state circuitry to transmit visual display of patient's blood pressure. Response of sphygmomanometer cuff and microphone provide input signals. Signals and their amplitudes, from turn-on time to turn-off time, are continuously fed to data transmitter which transmits to display device.

Improved First Pass Spiral Myocardial Perfusion Imaging with Variable Density Trajectories

PubMed Central

Salerno, Michael; Sica, Christopher; Kramer, Christopher M.; Meyer, Craig H.

2013-01-01

Purpose To develop and evaluate variable-density (VD) spiral first-pass perfusion pulse sequences for improved efficiency and off-resonance performance and to demonstrate the utility of an apodizing density compensation function (DCF) to improve SNR and reduce dark-rim artifact caused by cardiac motion and Gibbs Ringing. Methods Three variable density spiral trajectories were designed, simulated, and evaluated in 18 normal subjects, and in 8 patients with cardiac pathology on a 1.5T scanner. Results By utilizing a density compensation function (DCF) which intentionally apodizes the k-space data, the side-lobe amplitude of the theoretical PSF is reduced by 68%, with only a 13% increase in the FWHM of the main-lobe as compared to the same data corrected with a conventional VD DCF, and has an 8% higher resolution than a uniform density spiral with the same number of interleaves and readout duration. Furthermore, this strategy results in a greater than 60% increase in measured SNR as compared to the same VD spiral data corrected with a conventional DCF (p<0.01). Perfusion defects could be clearly visualized with minimal off-resonance and dark-rim artifacts. Conclusion VD spiral pulse sequences using an apodized DCF produce high-quality first-pass perfusion images with minimal dark-rim and off-resonance artifacts, high SNR and CNR and good delineation of resting perfusion abnormalities. PMID:23280884

Efficacy and mode of action of a noise-sensor light alarm to decrease noise in the pediatric intensive care unit: a prospective, randomized study.

PubMed

Jousselme, Chloé; Vialet, Renaud; Jouve, Elisabeth; Lagier, Pierre; Martin, Claude; Michel, Fabrice

2011-03-01

To determine whether a sound-activated light-alarm device could reduce the noise in the central area of our pediatric intensive care unit and to determine whether this reduction was significant enough to decrease the noise that could be perceived by a patient located in a nearby room. The secondary objective was to determine the mode of action of the device. In a 16-bed pediatric and neonatal intensive care unit, a large and clearly noticeable sound-activated light device was set in the noisiest part of the central area of our unit, and noise measurements were made in the central area and in a nearby room. In a prospective, quasi-experimental design, sound levels were compared across three different situations--no device present, device present and turned on, and device present but turned off--and noise level measurements were made over a total of 18 days. None. Setting a sound-activated light device on or off. When the device was present, the noise was about 2 dB lower in the central area and in a nearby room, but there was no difference in noise level with the device turned on vs. turned off. The noise decrease in the central area was of limited importance but was translated in a nearby room. The sound-activated light device did not directly decrease noise when turned on, but repetition of the visual signal throughout the day raised staff awareness of noise levels over time.

Identification of distant drug off-targets by direct superposition of binding pocket surfaces.

PubMed

Schumann, Marcel; Armen, Roger S

2013-01-01

Correctly predicting off-targets for a given molecular structure, which would have the ability to bind a large range of ligands, is both particularly difficult and important if they share no significant sequence or fold similarity with the respective molecular target ("distant off-targets"). A novel approach for identification of off-targets by direct superposition of protein binding pocket surfaces is presented and applied to a set of well-studied and highly relevant drug targets, including representative kinases and nuclear hormone receptors. The entire Protein Data Bank is searched for similar binding pockets and convincing distant off-target candidates were identified that share no significant sequence or fold similarity with the respective target structure. These putative target off-target pairs are further supported by the existence of compounds that bind strongly to both with high topological similarity, and in some cases, literature examples of individual compounds that bind to both. Also, our results clearly show that it is possible for binding pockets to exhibit a striking surface similarity, while the respective off-target shares neither significant sequence nor significant fold similarity with the respective molecular target ("distant off-target").

Identification of Distant Drug Off-Targets by Direct Superposition of Binding Pocket Surfaces

PubMed Central

Schumann, Marcel; Armen, Roger S.

2013-01-01

Correctly predicting off-targets for a given molecular structure, which would have the ability to bind a large range of ligands, is both particularly difficult and important if they share no significant sequence or fold similarity with the respective molecular target (“distant off-targets”). A novel approach for identification of off-targets by direct superposition of protein binding pocket surfaces is presented and applied to a set of well-studied and highly relevant drug targets, including representative kinases and nuclear hormone receptors. The entire Protein Data Bank is searched for similar binding pockets and convincing distant off-target candidates were identified that share no significant sequence or fold similarity with the respective target structure. These putative target off-target pairs are further supported by the existence of compounds that bind strongly to both with high topological similarity, and in some cases, literature examples of individual compounds that bind to both. Also, our results clearly show that it is possible for binding pockets to exhibit a striking surface similarity, while the respective off-target shares neither significant sequence nor significant fold similarity with the respective molecular target (“distant off-target”). PMID:24391782

Whole genome analysis of CRISPR Cas9 sgRNA off-target homologies via an efficient computational algorithm.

PubMed

Zhou, Hong; Zhou, Michael; Li, Daisy; Manthey, Joseph; Lioutikova, Ekaterina; Wang, Hong; Zeng, Xiao

2017-11-17

The beauty and power of the genome editing mechanism, CRISPR Cas9 endonuclease system, lies in the fact that it is RNA-programmable such that Cas9 can be guided to any genomic loci complementary to a 20-nt RNA, single guide RNA (sgRNA), to cleave double stranded DNA, allowing the introduction of wanted mutations. Unfortunately, it has been reported repeatedly that the sgRNA can also guide Cas9 to off-target sites where the DNA sequence is homologous to sgRNA. Using human genome and Streptococcus pyogenes Cas9 (SpCas9) as an example, this article mathematically analyzed the probabilities of off-target homologies of sgRNAs and discovered that for large genome size such as human genome, potential off-target homologies are inevitable for sgRNA selection. A highly efficient computationl algorithm was developed for whole genome sgRNA design and off-target homology searches. By means of a dynamically constructed sequence-indexed database and a simplified sequence alignment method, this algorithm achieves very high efficiency while guaranteeing the identification of all existing potential off-target homologies. Via this algorithm, 1,876,775 sgRNAs were designed for the 19,153 human mRNA genes and only two sgRNAs were found to be free of off-target homology. By means of the novel and efficient sgRNA homology search algorithm introduced in this article, genome wide sgRNA design and off-target analysis were conducted and the results confirmed the mathematical analysis that for a sgRNA sequence, it is almost impossible to escape potential off-target homologies. Future innovations on the CRISPR Cas9 gene editing technology need to focus on how to eliminate the Cas9 off-target activity.

Falling Outer Rotation Curves of Star-forming Galaxies at 0.6 ≲ z ≲ 2.6 Probed with KMOS3D and SINS/zC-SINF

NASA Astrophysics Data System (ADS)

Lang, Philipp; Förster Schreiber, Natascha M.; Genzel, Reinhard; Wuyts, Stijn; Wisnioski, Emily; Beifiori, Alessandra; Belli, Sirio; Bender, Ralf; Brammer, Gabe; Burkert, Andreas; Chan, Jeffrey; Davies, Ric; Fossati, Matteo; Galametz, Audrey; Kulkarni, Sandesh K.; Lutz, Dieter; Mendel, J. Trevor; Momcheva, Ivelina G.; Naab, Thorsten; Nelson, Erica J.; Saglia, Roberto P.; Seitz, Stella; Tacchella, Sandro; Tacconi, Linda J.; Tadaki, Ken-ichi; Übler, Hannah; van Dokkum, Pieter G.; Wilman, David J.

2017-05-01

We exploit the deep, resolved, Hα kinematic data from the KMOS3D and SINS/zC-SINF surveys to examine the largely unexplored outer-disk kinematics of star-forming galaxies (SFGs), out to the peak of cosmic star formation. Our sample contains 101 SFGs, representative of the more massive (9.3≲ {log}{M}* /{M}⊙ ≲ 11.5) main sequence population at 0.6 ≤ z ≤ 2.6. Through a novel stacking approach, we are able to constrain a representative rotation curve extending out to ˜4 effective radii. This average rotation curve exhibits a significant drop in rotation velocity beyond the turnover, with a slope of {{Δ }}V/{{Δ }}R=-{0.26}-0.09+0.10 in units of normalized coordinates V/V max and R/R turn. This result confirms that the fall-off seen in some individual galaxies is a common feature of our sample of high-z disks. The outer fall-off strikingly deviates from the flat or mildly rising rotation curves of local spiral galaxies that have similar masses. Through a comparison with models that include baryons and dark matter, we demonstrate that the falling stacked rotation curve is consistent with a high mass fraction of baryons, relative to the total dark matter halo (m d ≳ 0.05), in combination with a sizeable level of pressure support in the outer disk. These findings agree with recent studies demonstrating that high-z star-forming disks are strongly baryon-dominated within the disk scale, and furthermore suggest that pressure gradients caused by large, turbulent gas motions are present even in their outer disks. These results are largely independent of our model assumptions, such as the presence of stellar bulges, the effect of adiabatic contraction, and variations in halo concentration.

A simple acquisition strategy to avoid off-resonance blurring in spiral imaging with redundant spiral-in/out k-space trajectories

PubMed Central

Fielden, Samuel W.; Meyer, Craig H.

2014-01-01

Purpose The major hurdle to widespread adoption of spiral trajectories has been their poor off-resonance performance. Here we present a self-correcting spiral k-space trajectory that avoids much of the well-known spiral blurring during data acquisition. Theory and Methods In comparison with a traditional spiral-out trajectory, the spiral-in/out trajectory has improved off-resonance performance. By combining two spiral-in/out acquisitions, one rotated 180° in k-space compared to the other, multi-shot spiral-in/out artifacts are eliminated. A phantom was scanned with the center frequency manually tuned 20, 40, 80, and 160 Hz off-resonance with both a spiral-out gradient echo sequence and the redundant spiral-in/out sequence. The phantom was also imaged in an oblique orientation in order to demonstrate improved concomitant gradient field performance of the sequence, and was additionally incorporated into a spiral turbo spin echo sequence for brain imaging. Results Phantom studies with manually-tuned off-resonance agree well with theoretical calculations, showing that moderate off-resonance is well-corrected by this acquisition scheme. Blur due to concomitant fields is reduced, and good results are obtained in vivo. Conclusion The redundant spiral-in/out trajectory results in less image blur for a given readout length than a traditional spiral-out scan, reducing the need for complex off-resonance correction algorithms. PMID:24604539

A simple acquisition strategy to avoid off-resonance blurring in spiral imaging with redundant spiral-in/out k-space trajectories.

PubMed

Fielden, Samuel W; Meyer, Craig H

2015-02-01

The major hurdle to widespread adoption of spiral trajectories has been their poor off-resonance performance. Here we present a self-correcting spiral k-space trajectory that avoids much of the well-known spiral blurring during data acquisition. In comparison with a traditional spiral-out trajectory, the spiral-in/out trajectory has improved off-resonance performance. By combining two spiral-in/out acquisitions, one rotated 180° in k-space compared with the other, multishot spiral-in/out artifacts are eliminated. A phantom was scanned with the center frequency manually tuned 20, 40, 80, and 160 Hz off-resonance with both a spiral-out gradient echo sequence and the redundant spiral-in/out sequence. The phantom was also imaged in an oblique orientation in order to demonstrate improved concomitant gradient field performance of the sequence. Additionally, the trajectory was incorporated into a spiral turbo spin echo sequence for brain imaging. Phantom studies with manually tuned off-resonance agree well with theoretical calculations, showing that moderate off-resonance is well-corrected by this acquisition scheme. Blur due to concomitant fields is reduced, and good results are obtained in vivo. The redundant spiral-in/out trajectory results in less image blur for a given readout length than a traditional spiral-out scan, reducing the need for complex off-resonance correction algorithms. © 2014 Wiley Periodicals, Inc.

The Big Mac Attack.

ERIC Educational Resources Information Center

Bushweller, Kevin

1995-01-01

Schools are increasingly turning to fast-food restaurants such as Taco Bell, McDonald's, and Pizza Hut to fill the stomachs of kids turned off by standard school lunches. Kids are delighted, but critics say fast-food infiltration of school cafeterias encourages poor nutrition. Schools might consider adopting lighter fast-food fare or starting…

Symptom Induction and De-escalation in the Treatment of Panic Attacks.

ERIC Educational Resources Information Center

Dattilio, Frank M.

1990-01-01

Describes technique known as symptom induction and de-escalation for panic attacks in which goal is to reproduce the type of situation that may precipitate an attack and then to show the client how the attacks can be "turned on" as well as "turned off." (ABL)

Supramolecular nano-sniffers for ultrasensitive detection of formaldehyde.

PubMed

Akshath, Uchangi Satyaprasad; Bhatt, Praveena

2018-02-15

Supramolecular nanoparticle hybrids for biosensing of analytes have been a major focus due to their tunable optical and surface properties. Quantum dots-Gold nanoparticle (QDs-GNP) based FRET probes involving turn on/off principles have gained immense interest due to their specificity and sensitivity. Recent focus is on applying these supramolecular hybrids for enzyme operated biosensors that can specifically turn-on fluorescence induced by co-factor or product formed from enzymatic reaction. The present study focuses on locking and unlocking the interaction between QD-GNP pair leading to differential fluorescent properties. Cationic GNPs efficiently quenched the anionic QD fluorescence by forming nanoparticle hybrid. Quenching interaction between QD-GNP pair was unlocked by NADH leading to QD fluorescence turn-on. This phenomenon was applied for the successful detection of formaldehyde using NAD + dependent formaldehyde dehydrogenase. The proposed nano-sniffer could successfully detect formaldehyde from 0.001 to 100000ng/mL (R 2 = 0.9339) by the turn off-turn on principle. It could also detect formaldehyde in fruit juice and wine samples indicating its stability and sensitivity in real samples. The proposed nanoprobe can have wide applications in developing enzyme biosensors in future. Copyright © 2017 Elsevier B.V. All rights reserved.

78 FR 23691 - Airworthiness Directives; the Boeing Company

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-22

... off electrical power to the in-flight entertainment (IFE) systems and other non-essential electrical...., Washington, DC 20590. FOR FURTHER INFORMATION CONTACT: Ray Mei, Aerospace Engineer, Systems and Equipment... circuit breaker, relays, and wiring to allow the flightcrew to turn off electrical power to the IFE...

Cooling system for radiator and condenser of vehicles with an air conditioner and method of operating the same

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shimada, Y.; Obata, Y.; Takeoka, T.

1987-04-21

A cooling system is described for radiator and condenser of vehicles with an air conditioner having a first blower and a second blower for cooling the radiator and the condenser so as to cool the engine cooling water and so as to condense the coolant, and a cooling cycle operation switch which comprises: (a) engine cooling water temperature switch (SW1) connected between a power supply and the first blower and turned on and off in accordance with high and low temperature conditions of the engine cooling water; (b) relay switching means for controlling the first and second blowers in accordancemore » with the on-off conditions of the cooling cycle operation switch; and (c) a control circuit having an on-off switch and a solenoid and connected between the relay switching means and either the first blower or the second blower, the solenoid of the control circuit being connected to switches (SW3, SW4 and SW5) for electrical equipment such as headlights, wipers; whereby, when any one of the switches for the electrical equipment of the vehicle is turned off, the first and second blowers are operated at normal speed through the relay switching means and the control circuit, upon the operation of the cooling cycle operation switch, while when any one of the switches for the electrical equipment is turned on, the first blower is on-off controlled through the engine cooling water temperature switch (SW1) and the second blower remains operated through the relay switching means.« less

Fluorescence turn-on responses of anionic and cationic conjugated polymers toward proteins: effect of electrostatic and hydrophobic interactions.

PubMed

Pu, Kan-Yi; Liu, Bin

2010-03-11

Cationic and anionic poly(fluorenyleneethynylene-alt-benzothiadiazole)s (PFEBTs) are designed and synthesized via Sonagashira coupling reaction to show light-up signatures toward proteins. Due to the charge transfer character of the excited states, the fluorescence of PFEBTs is very weak in aqueous solution, while their yellow fluorescence can be enhanced by polymer aggregation. PFEBTs show fluorescence turn-on rather than fluorescence quenching upon complexation with proteins. Both electrostatic and hydrophobic interactions between PFEBTs and proteins are found to improve the polymer fluorescence, the extent of which is dependent on the nature of the polymer and the protein. Changes in solution pH adjust the net charges of proteins, providing an effective way to manipulate electrostatic interactions and in turn the increment in the polymer fluorescence. In addition, the effect of protein digestion on the fluorescence of polymer/protein complexes is probed. The results indicate that electrostatic interaction induced polymer fluorescence increase cannot be substantially reduced through cleaving protein into peptide fragments. In contrast, hydrophobic interactions, mainly determined by the hydrophobicity of proteins, can be minimized by digestion, imparting a light-off signature for the polymer/protein complexes. This study thus not only highlights the opportunities of exerting nonspecific interactions for protein sensing but also reveals significant implications for biosensor design.

Utilizing Schottky barriers to suppress short-channel effects in organic transistors

NASA Astrophysics Data System (ADS)

Fernández, Anton F.; Zojer, Karin

2017-10-01

Transistors with short channel lengths exhibit profound deviations from the ideally expected behavior. One of the undesired short-channel effects is an enlarged OFF current that is associated with a premature turn on of the transistor. We present an efficient approach to suppress the OFF current, defined as the current at zero gate source bias, in short-channel organic transistors. We employ two-dimensional device simulations based on the drift-diffusion model to demonstrate that intentionally incorporating a Schottky barrier for injection enhances the ON-OFF ratio in both staggered and coplanar transistor architectures. The Schottky barrier is identified to directly counteract the origin of enlarged OFF currents: Short channels promote a drain-induced barrier lowering. The latter permits unhindered injection of charges even at reverse gate-source bias. An additional Schottky barrier hampers injection for such points of operations. We explain how it is possible to find the Schottky barrier of the smallest height necessary to exactly compensate for the premature turn on. This approach offers a substantial enhancement of the ON-OFF ratio. We show that this roots in the fact that such optimal barrier heights offer an excellent compromise between an OFF current diminished by orders of magnitude and an only slightly reduced ON current.

A novel deletion/insertion mutation in the mRNA transcribed from one {alpha}1(I) collagen allele in a family with dominant type III OI and germline mosaicism

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wang, O.; Masters, C.; Lewis, M.B.

1994-09-01

In an 8-year-old girl and her father, both of whom have severe type III OI, we have previously used RNA/RNA hybrid analysis to demonstrate a mismatch in the region of {alpha}1(I) mRNA coding for aa 558-861. We used SSCP to further localize the abnormality to a subregion coding for aa 579-679. This region was subcloned and sequenced. Each patient`s cDNA has a deletion of the sequences coding for the last residue of exon 34, and all of exons 35 and 36 (aa 604-639), followed by an insertion of 156 nt from the 3{prime}-end of intron 36. PCR amplification of leukocytemore » DNA from the patients and the clinically normal paternal grandmother yielded two fragments: a 1007 bp fragment predicted from normal genomic sequences and a 445 bp fragment. Subcloning and sequencing of the shorter genomic PCR product confirmed the presence of a 565 bp genomic deletion from the end of exon 34 to the middle of intron 36. The abnormal protein is apparently synthesized and incorporated into helix. The inserted nucleotides are in frame with the collagenous sequence and contain no stop codons. They encode a 52 aa non-collagenous region. The fibroblast procollagen of the patients has both normal and electrophoretically delayed pro{alpha}(I) bands. The electrophoretically delayed procollagen is very sensitive to pepsin or trypsin digestion, as predicted by its non-collagenous sequence, and cannot be visualized as collagen. This unique OI collagen mutation is an excellent candidate for molecular targeting to {open_quotes}turn off{close_quotes} a dominant mutant allele.« less

14 CFR 29.143 - Controllability and maneuverability.

Code of Federal Regulations, 2011 CFR

2011-01-01

...) Critical weight; (2) Critical center of gravity; (3) Critical rotor r.p.m.; and (4) Power off (except for helicopters demonstrating compliance with paragraph (f) of this section) and power on. (c) Wind velocities...; (iii) Level flight; (iv) Turning flight; (v) Autorotation; and (vi) Landing (power on and power off...

Problematic topic transitions in dysarthric conversation.

PubMed

Bloch, Steven; Saldert, Charlotta; Ferm, Ulrika

2015-01-01

This study examined the nature of topic transition problems associated with acquired progressive dysarthric speech in the everyday conversation of people with motor neurone disease. Using conversation analytic methods, a video collection of five naturally occurring problematic topic transitions was identified, transcribed and analysed. These were extracted from a main collection of over 200 other-initiated repair sequences and a sub-set of 15 problematic topic transition sequences. The sequences were analysed with reference to how the participants both identified and resolved the problems. Analysis revealed that topic transition by people with dysarthria can prove problematic. Conversation partners may find transitions problematic not only because of speech intelligibility but also because of a sequential disjuncture between the dysarthric speech turn and whatever topic has come prior. In addition the treatment of problematic topic transition as a complaint reveals the potential vulnerability of people with dysarthria to judgements of competence. These findings have implications for how dysarthria is conceptualized and how specific actions in conversation, such as topic transition, might be suitable targets for clinical intervention.

Base drive circuit for a four-terminal power Darlington

DOEpatents

Lee, Fred C.; Carter, Roy A.

1983-01-01

A high power switching circuit which utilizes a four-terminal Darlington transistor block to improve switching speed, particularly in rapid turn-off. Two independent reverse drive currents are utilized during turn off in order to expel the minority carriers of the Darlington pair at their own charge sweep-out rate. The reverse drive current may be provided by a current transformer, the secondary of which is tapped to the base terminal of the power stage of the Darlington block. In one application, the switching circuit is used in each power switching element in a chopper-inverter drive of an electric vehicle propulsion system.

Why nature doesn't turn off neurosis.

PubMed

Badalamenti, Anthony F

2013-03-01

This paper develops an integration of psychoanalytic and wisdom tradition concepts to answer the question as to why nature does not turn off neurosis, The proposed answer is that nature wants a person to exploit the neurosis for two gains, one being the increase in adaptive capacity resulting from releasing it and the second involving the difficulty in the release itself, the latter related to gains proffered by the world's wisdom traditions. These see a movement from the psyche's creation by passive, unconscious means of finite promise rooted in parental love to creation by active, consciously chosen means of unlimited promise involving a direct relationship with nature and the Cosmos.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Parada, Javiera; Richer, Harvey; Heyl, Jeremy

Blue stragglers (BSS) are stars whose position in the color–magnitude diagram (CMD) places them above the main sequence (MS) turn-off (TO) point of a star cluster. Using data from the core of 47 Tuc in the ultraviolet (UV), we have identified various stellar populations in the CMD, and used their radial distributions to study the evolution and origin of BSS, and obtain a dynamical estimate of the mass of BSS systems. When we separate the BSS into two samples by their magnitude, we find that the bright BSS show a much more centrally concentrated radial distribution and thus higher massmore » estimate (over twice the TO mass for these BSS systems), suggesting an origin involving triple or multiple stellar systems. In contrast, the faint BSS are less concentrated, with a radial distribution similar to the MS binaries, pointing to the MS binaries as the likely progenitors of these BSS. Putting our data together with available photometric data in the visible and using MESA evolutionary models, we calculate the expected number of stars in each evolutionary stage for the normal evolution of stars and the number of stars coming from the evolution of BSS. The results indicate that BSS have a post-MS evolution comparable to that of a normal star of the same mass and a MS BSS lifetime of about 200–300 Myr. We also find that the excess population of asymptotic giant branch stars in 47 Tuc is due to evolved BSS.« less

UIT Observations of Early-Type Galaxies and Analysis of the FUSE Spectrum of a Subdwarf B Star

NASA Technical Reports Server (NTRS)

Ohl, Raymond G.; Krebs, Carolyn (Technical Monitor)

2001-01-01

This work covers Ultraviolet Imaging Telescope (UIT) observations of early-type galaxies (155 nm) and Far Ultraviolet Spectroscopic Explorer (FUSE) spectra of a Galactic subdwarf B star (sdB). Early UV space astronomy missions revealed that early-type galaxies harbor a population of stars with effective temperatures greater than that of the main sequence turn-off (about 6,000 K) and UV emission that is very sensitive to characteristics of the stellar population. We present UV (155 nm) surface photometry and UV-B color profiles for 8 E and SO galaxies observed by UIT. Some objects have de Vaucouleurs surface brightness profiles, while others have disk-like profiles, but we find no other evidence for the presence of a disk or young, massive stars. There is a wide range of UV-B color gradients, but there is no correlation with metallicity gradients. SdB stars are the leading candidate UV emitters in old, high metallicity stellar populations (e.g., early-type galaxies). We observed the Galactic sdB star PG0749+658 with FUSE and derived abundances with the aim of constraining models of the heavy element distribution in sdB atmospheres. All of the elements measured are depleted with respect to solar, except for Cr and Mn, which are about solar, and Ni, which is enhanced. This work was supported in part by NASA grants NAG5-700 and NAG5-6403 to the University of Virginia and NAS5-32985 to Johns Hopkins University.

Chemical Evolution and History of Star Formation in the Large Magellanic Cloud

NASA Astrophysics Data System (ADS)

Gustafsson, Bengt

1995-07-01

Large scale processes controlling star formation and nucleosynthesis are fundamental but poorly understood. This is especially true for external galaxies. A detailed study of individual main sequence stars in the LMC Bar is proposed. The LMC is close enough to allow this, has considerable spread in stellar ages and a structure permitting identification of stellar populations and their structural features. The Bar presumably plays a dominant role in the chemical and dynamical evolution of the galaxy. Our knowledge is, at best, based on educated guesses. Still, the major population of the Bar is quite old, and many member stars are relatively evolved. The Bar seems to contain stars similar to those of Intermediate to Extreme Pop II in the Galaxy. We want to study the history of star formation, chemical evolution and initial mass function of the population dominating the Bar. We will use field stars close to the turn off point in the HR diagram. From earlier studies, we know that 250-500 such stars are available for uvby photometry in the PC field. We aim at an accuracy of 0.1 -0.2 dex in Me/H and 25% or better in relative ages. This requires an accuracy of about 0.02 mag in the uvby indices, which can be reached, taking into account errors in calibration, flat fielding, guiding and problems due to crowding. For a study of the luminosity function fainter stars will be included as well. Calibration fields are available in Omega Cen and M 67.

Discovery of a stellar overdensity in Eridanus-Phoenix in the dark energy survey

DOE PAGES

Li, T. S.; Balbinot, E.; Mondrik, N.; ...

2016-01-27

We report the discovery of an excess of main sequence turn-off stars in the direction of the constellations of Eridanus and Phoenix from the first year data of the Dark Energy Survey (DES). The Eridanus-Phoenix (EriPhe) overdensity is centered around l~285 deg and b~-60 deg and spans at least 30 deg in longitude and 10 deg in latitude. The Poisson significance of the detection is at least 9 sigma. The stellar population in the overdense region is similar in brightness and color to that of the nearby globular cluster NGC 1261, indicating that the heliocentric distance of EriPhe is aboutmore » d~16 kpc. The extent of EriPhe in projection is therefore at least ~4 kpc by ~3 kpc. On the sky, this overdensity is located between NGC 1261 and a new stellar stream discovered by DES at a similar heliocentric distance, the so-called Phoenix Stream. Given their similar distance and proximity to each other, it is possible that these three structures may be kinematically associated. Alternatively, the EriPhe overdensity is morphologically similar to the Virgo overdensity and the Hercules-Aquila cloud, which also lie at a similar Galactocentric distance. These three overdensities lie along a polar plane separated by ~120 deg and may share a common origin. Spectroscopic follow-up observations of the stars in EriPhe are required to fully understand the nature of this overdensity.« less

Stellar Variability in the Intermediate Age Cluster NGC 1846

NASA Astrophysics Data System (ADS)

Pajkos, Michael A.; Salinas, Ricardo; Vivas, Anna Katherina; Strader, Jay; Contreras, Rodrigo

2017-01-01

The existence of multiple stellar populations in Galactic globular clusters is considered a widespread phenomenon, with only a few possible exceptions. In the LMC intermediate-age globular clusters, the presence of extended main sequence turn off points (MSTOs), initially interpreted as evidence for multiple stellar populations, is now under scrutiny and stellar rotation has emerged as an alternative explanation. Here we propose yet another ingredient to this puzzle: the fact that the MSTO of these clusters passes through the instability strip making stellar variability a new alternative to explain this phenomenon. We report the first in-depth characterization of the variability, at the MSTO level, in any LMC cluster, and assess the role of variability masquerading as multiple stellar populations. We used the Gemini-S/GMOS to obtain time series photometry of NGC 1846. Using differencing image analysis, we identified 90 variables in the r-band, 68 of which were also found in the g-band. Of these 68, 57 were δ-scuti—with 35 having full phase coverage and 22 without. The average full period (Pfull) was 1.93 ± 0.79 hours. Furthermore, two eclipsing binaries and two RR Lyrae identified by OGLE were recovered. We conclude that not enough variables were found to provide a statistically significant impact on the extended MSTO, nor to explain the bifurcation of MSTO in NGC 1846. But the effect of variable stars could still be a viable explanation on clusters where only a hint of a MS extension is seen.

The effect of short-term auditory deprivation on the control of intraoral pressure in pediatric cochlear implant users.

PubMed

Jones, David L; Gao, Sujuan; Svirsky, Mario A

2003-06-01

The purpose of this study was to determine whether 2 speech measures (peak intraoral air pressure [IOP] and IOP duration) obtained during the production of intervocalic stops would be altered as a function of the presence or absence of auditory stimulation provided by a cochlear implant (CI). Five pediatric CI users were required to produce repetitions of the words puppy and baby with their CIs turned on. The CIs were then turned off for 1 hr, at which time the speech sample was repeated with the CI still turned off. Seven children with normal hearing formed a comparison group. They were also tested twice, with a 1-hr intermediate interval. IOP and IOP duration were measured for the medial consonant in both auditory conditions. The results show that auditory condition affected peak IOP more so than IOP duration. Peak IOP was greater for /p/ than /b/ with the CI off, but some participants reduced or reversed this contrast when the CI was on. The findings suggest that different speakers with CIs may use different speech production strategies as they learn to use the auditory signal for speech.

Distinct retinal pathways drive spatial orientation behaviors in zebrafish navigation.

PubMed

Burgess, Harold A; Schoch, Hannah; Granato, Michael

2010-02-23

Navigation requires animals to adjust ongoing movements in response to pertinent features of the environment and select between competing target cues. The neurobiological basis of navigational behavior in vertebrates is hard to analyze, partly because underlying neural circuits are experience dependent. Phototaxis in zebrafish is a hardwired navigational behavior, performed at a stage when larvae swim by using a small repertoire of stereotyped movements. We established conditions to elicit robust phototaxis behavior and found that zebrafish larvae deploy directional orienting maneuvers and regulate forward swimming speed to navigate toward a target light. Using genetic analysis and targeted laser ablations, we show that retinal ON and OFF pathways play distinct roles during phototaxis. The retinal OFF pathway controls turn movements via retinotectal projections and establishes correct orientation by causing larvae to turn away from nontarget areas. In contrast, the retinal ON pathway activates the serotonergic system to trigger rapid forward swimming toward the target. Computational simulation of phototaxis with an OFF-turn, ON-approach algorithm verifies that our model accounts for key features of phototaxis and provides a simple and robust mechanism for behavioral choice between competing targets. Copyright 2010 Elsevier Ltd. All rights reserved.

Dynamics in hybrid complex systems of switches and oscillators

NASA Astrophysics Data System (ADS)

Taylor, Dane; Fertig, Elana J.; Restrepo, Juan G.

2013-09-01

While considerable progress has been made in the analysis of large systems containing a single type of coupled dynamical component (e.g., coupled oscillators or coupled switches), systems containing diverse components (e.g., both oscillators and switches) have received much less attention. We analyze large, hybrid systems of interconnected Kuramoto oscillators and Hopfield switches with positive feedback. In this system, oscillator synchronization promotes switches to turn on. In turn, when switches turn on, they enhance the synchrony of the oscillators to which they are coupled. Depending on the choice of parameters, we find theoretically coexisting stable solutions with either (i) incoherent oscillators and all switches permanently off, (ii) synchronized oscillators and all switches permanently on, or (iii) synchronized oscillators and switches that periodically alternate between the on and off states. Numerical experiments confirm these predictions. We discuss how transitions between these steady state solutions can be onset deterministically through dynamic bifurcations or spontaneously due to finite-size fluctuations.

Smart home design for electronic devices monitoring based wireless gateway network using cisco packet tracer

NASA Astrophysics Data System (ADS)

Sihombing, Oloan; Zendrato, Niskarto; Laia, Yonata; Nababan, Marlince; Sitanggang, Delima; Purba, Windania; Batubara, Diarmansyah; Aisyah, Siti; Indra, Evta; Siregar, Saut

2018-04-01

In the era of technological development today, the technology has become the need for the life of today's society. One is needed to create a smart home in turning on and off electronic devices via smartphone. So far in turning off and turning the home electronic device is done by pressing the switch or remote button, so in control of electronic device control less effective. The home smart design is done by simulation concept by testing system, network configuration, and wireless home gateway computer network equipment required by a smart home network on cisco packet tracer using Internet Thing (IoT) control. In testing the IoT home network wireless network gateway system, multiple electronic devices can be controlled and monitored via smartphone based on predefined configuration conditions. With the Smart Ho me can potentially increase energy efficiency, decrease energy usage costs, control electronics and change the role of residents.

A screen of chemical modifications identifies position-specific modification by UNA to most potently reduce siRNA off-target effects

PubMed Central

Bramsen, Jesper B.; Pakula, Malgorzata M.; Hansen, Thomas B.; Bus, Claus; Langkjær, Niels; Odadzic, Dalibor; Smicius, Romualdas; Wengel, Suzy L.; Chattopadhyaya, Jyoti; Engels, Joachim W.; Herdewijn, Piet; Wengel, Jesper; Kjems, Jørgen

2010-01-01

Small interfering RNAs (siRNAs) are now established as the preferred tool to inhibit gene function in mammalian cells yet trigger unintended gene silencing due to their inherent miRNA-like behavior. Such off-target effects are primarily mediated by the sequence-specific interaction between the siRNA seed regions (position 2–8 of either siRNA strand counting from the 5′-end) and complementary sequences in the 3′UTR of (off-) targets. It was previously shown that chemical modification of siRNAs can reduce off-targeting but only very few modifications have been tested leaving more to be identified. Here we developed a luciferase reporter-based assay suitable to monitor siRNA off-targeting in a high throughput manner using stable cell lines. We investigated the impact of chemically modifying single nucleotide positions within the siRNA seed on siRNA function and off-targeting using 10 different types of chemical modifications, three different target sequences and three siRNA concentrations. We found several differently modified siRNAs to exercise reduced off-targeting yet incorporation of the strongly destabilizing unlocked nucleic acid (UNA) modification into position 7 of the siRNA most potently reduced off-targeting for all tested sequences. Notably, such position-specific destabilization of siRNA–target interactions did not significantly reduce siRNA potency and is therefore well suited for future siRNA designs especially for applications in vivo where siRNA concentrations, expectedly, will be low. PMID:20453030

Sulfur and iron cycling in deep-subsurface, coal bed-containing sediments off Shimokita (Japan)

NASA Astrophysics Data System (ADS)

Riedinger, N.; Smirnoff, M. N.; Gilhooly, W.; Phillips, S. C.; Lyons, T. W.; 337 Scientific Party, I.

2013-12-01

The main goal of IODP Expedition 337 was the identification and characterization of the deep coal bed biosphere and hydrocarbon system off the Shimokita Peninsula (Japan) in the northwestern Pacific using the D/V Chikyu. To accomplish this scientific objective, it was also necessary to investigate the inorganic biogeochemistry in order to identify possible electron acceptors and bio-essential nutrients. These biogeochemical parameters greatly influence both, the composition and abundance of microbial communities as well as the organic carbon cycle. In turn, the microbially mediated carbon cycle influences the diagenetic reactions in the subsurface, thus, altering geochemical and physical characteristics of the material. Here we present results from metal and sulfur geochemical analyses from the deep-subsurface sediments (about 1250 to 2466 mbsf) at Site C0020 off Shimokita. The measured concentrations of acid volatile sulfur (AVS) as well as chromium reducible sulfur (CRS) reflect the alteration of iron oxides to iron sulfides and indicate that the main sulfur-bearing phase in the investigated sediments is pyrite. Concentrations of intermediate sulfur species are minor and occur mainly in the coal-bearing interval. Our data show that the uppermost sediments contain higher amounts of pyrite (up to 1.2 wt.%) with an average of 0.5 wt.% compared to the deeper deposits (below about 1800 mbsf), which show an average of 0.16 wt.%. In contrast, iron oxide concentrations are highest in the deeper sediment sections (up to 0.4%), where pyrite concentrations are low. The alteration of iron oxides to sulfides in theses lower section was probably governed by the amount of available sulfide in the pore water. The occurrence of (bio-)reactive iron phases in these deeply buried sediments has implications for the deep biosphere as those minerals have the potential to serve as electron acceptors during burial, including reactions involving deep sourced electron donors, such as hydrogen and methane - related to the coal bed as the potential source. Thus, the deep subsurface coal beds off Shimokita provide an ideal environment to investigate microbial and metal interactions under extreme conditions.

Fast Regulation of Vertical Squat Jump during Push-Off in Skilled Jumpers.

PubMed

Fargier, Patrick; Massarelli, Raphael; Rabahi, Tahar; Gemignani, Angelo; Fargier, Emile

2016-01-01

The height of a maximum Vertical Squat Jump (VSJ) reflects the useful power produced by a jumper during the push-off phase. In turn this partly depends on the coordination of the jumper's segmental rotations at each instant. The physical system constituted by the jumper has been shown to be very sensitive to perturbations and furthermore the movement is realized in a very short time (ca. 300 ms), compared to the timing of known feedback loops. However, the dynamics of the segmental coordination and its efficiency in relation to energetics at each instant of the push-off phase still remained to be clarified. Their study was the main purpose of the present research. Eight young adult volunteers (males) performed maximal VSJ. They were skilled in jumping according to their sport activities (track and field or volleyball). A video analysis on the kinematics of the jump determined the influence of the jumpers' segments rotation on the vertical velocity and acceleration of the body mass center (MC). The efficiency in the production of useful power at the jumpers' MC level, by the rotation of the segments, was measured in consequence. The results showed a great variability in the segmental movements of the eight jumpers, but homogeneity in the overall evolution of these movements with three consecutive types of coordination in the second part of the push-off (lasting roughly 0.16 s). Further analyses gave insights on the regulation of the push-off, suggesting that very fast regulation(s) of the VSJ may be supported by: (a) the adaptation of the motor cerebral programming to the jumper's physical characteristics; (b) the control of the initial posture; and (c) the jumper's perception of the position of his MC relative to the ground reaction force, during push-off, to reduce energetic losses.

The evolving star formation rate: M⋆ relation and sSFR since z ≃ 5 from the VUDS spectroscopic survey

NASA Astrophysics Data System (ADS)

Tasca, L. A. M.; Le Fèvre, O.; Hathi, N. P.; Schaerer, D.; Ilbert, O.; Zamorani, G.; Lemaux, B. C.; Cassata, P.; Garilli, B.; Le Brun, V.; Maccagni, D.; Pentericci, L.; Thomas, R.; Vanzella, E.; Zucca, E.; Amorin, R.; Bardelli, S.; Cassarà, L. P.; Castellano, M.; Cimatti, A.; Cucciati, O.; Durkalec, A.; Fontana, A.; Giavalisco, M.; Grazian, A.; Paltani, S.; Ribeiro, B.; Scodeggio, M.; Sommariva, V.; Talia, M.; Tresse, L.; Vergani, D.; Capak, P.; Charlot, S.; Contini, T.; de la Torre, S.; Dunlop, J.; Fotopoulou, S.; Koekemoer, A.; López-Sanjuan, C.; Mellier, Y.; Pforr, J.; Salvato, M.; Scoville, N.; Taniguchi, Y.; Wang, P. W.

2015-09-01

We study the evolution of the star formation rate (SFR) - stellar mass (M⋆) relation and specific star formation rate (sSFR) of star-forming galaxies (SFGs) since a redshift z ≃ 5.5 using 2435 (4531) galaxies with highly reliable spectroscopic redshifts in the VIMOS Ultra-Deep Survey (VUDS). It is the first time that these relations can be followed over such a large redshift range from a single homogeneously selected sample of galaxies with spectroscopic redshifts. The log (SFR) - log (M⋆) relation for SFGs remains roughly linear all the way up to z = 5, but the SFR steadily increases at fixed mass with increasing redshift. We find that for stellar masses M⋆ ≥ 3.2 × 109M⊙ the SFR increases by a factor of ~13 between z = 0.4 and z = 2.3. Weextend this relation up to z = 5, finding an additional increase in SFR by a factor of 1.7 from z = 2.3 to z = 4.8 for masses M⋆ ≥ 1010M⊙. We observe a turn-off in the SFR-M⋆ relation at the highest mass end up to a redshift z ~ 3.5. We interpret this turn-off as the signature of a strong on-going quenching mechanism and rapid mass growth. The sSFR increases strongly up to z ~ 2, but it grows much less rapidly in 2

Basic Energy Conservation and Management Part 1: Looking at Lighting

ERIC Educational Resources Information Center

Krueger, Glenn

2012-01-01

Reducing school district energy expenditures has become a universal goal. However, school board members, superintendents, and directors of buildings and grounds are often unaware of the many options available to conserve energy. School energy conservation used to be relatively simple: turn off the lights and turn down the heat in the winter and…

40 CFR 1066.845 - AC17 air conditioning efficiency test procedure.

Code of Federal Regulations, 2014 CFR

2014-07-01

... solar heating is disabled for certain test intervals as described in this section. (d) Interior air... vehicle's windows and operate the vehicle over a preconditioning UDDS with no solar heating and with the... cooling fans. (3) Turn on solar heating within one minute after turning off the engine. Once the solar...

Science Education at Fermilab Program Search

Science.gov Websites

JavaScript is Turned Off or Not Supported in Your Browser. To search for programs go to the Non -Javascript Search or turn on Javascript and reload this page. Programs | Science Adventures | Calendar | Undergraduates Fermilab Ed Site Search Google Custom Search Programs: Introducing You to the World of Science

EFRH-phage immunization of Alzheimer's disease animal model improves behavioral performance in Morris water maze trials.

PubMed

Lavie, Vered; Becker, Maria; Cohen-Kupiec, Rachel; Yacoby, Iftach; Koppel, Rela; Wedenig, Manuela; Hutter-Paier, Birgit; Solomon, Beka

2004-01-01

We have developed an immunization procedure for the production of effective anti-beta-amyloid (anti-Abeta) antibodies, using filamentous phage displaying only 4 amino acids. The EFRH sequence, encompassing amino acids 3-6 of the 42 residues of Abeta peptide, was found previously to be the main regulatory site for amyloid modulation and the epitope of anti-aggregating antibodies. Engineered filamentous phage enable the display of various numbers of EFRH copies on the phage and serve as potent carriers of antigens. In the present study we have found that phage displaying high EFRH copy number are effective in eliciting humoral response against the EFRH sequence, which in turn relieves the amyloid burden in the brains of amyloid precursor protein Tg mice and improves their ability to perform cognitive tasks. Copyright 2004 Humana Press Inc.

Describing-function analysis of a ripple regulator with slew-rate limits and time delays

NASA Technical Reports Server (NTRS)

Wester, Gene W.

1990-01-01

The effects of time delays and slew-rate limits on the steady-state operating points and performance of a free-running ripple regulator are evaluated using describing-function analysis. The describing function of an ideal comparator (no time delays or slew rate limits) has no phase shift and is independent of frequency. It is found that turn-on delay and turn-off delay have different effects on gain and phase and cannot be combined. Comparator hysteresis affects both gain and phase; likewise, time delays generally affect both gain and phase. It is found that the effective time delay around the feedback loop is one half the sum of turn-on and turn-off delays, regardless of whether the delays are caused by storage time or slew rate limits. Expressions are formulated for the switching frequency, switch duty ratio, dc output, and output ripple. For the case of no hysteresis, a simple, graphical solution for the switching frequency is possible, and the resulting switching frequency is independent of first-order variations of input or load.

Differences of ballet turns (pirouette) performance between experienced and novice ballet dancers.

PubMed

Lin, Chia-Wei; Chen, Shing-Jye; Su, Fong-Chin; Wu, Hong-Wen; Lin, Cheng-Feng

2014-09-01

This study investigated the different postural control strategies exhibited by experienced and novice dancers in ballet turns (pirouettes). Thirteen novice and 13 experienced dancers performed ballet turns with dominant-leg support. The peak push force was measured in the double-leg support phase. The inclination angles of rotation axis with respect to vertical axis were calculated in the early single-leg support phase as well as the initiation sequence of ankle, knee, and hip joints on the supporting leg. Moreover, the anchoring index of the head was computed in the transverse plane during turning. The novice dancers applied a greater push force, an increased inclination angle of rotation axis, and an insufficient proximal-to-distal extension sequence pattern. The novice dancers also had a smaller head-anchoring index compared with experienced dancers, which meant novice dancers were not using a space target as a stability reference. A poorer performance in novice dancers could result from higher push force in propulsion, lack of a "proximal-to-distal extension sequence" pattern, and lack of visual spotting for postural stability. Training on sequential initiation of lower-extremity joints and rehearsal of visual spotting are essential for novice dancers to obtain better performance on ballet turns.

Novel trench gate field stop IGBT with trench shorted anode

NASA Astrophysics Data System (ADS)

Xudong, Chen; Jianbing, Cheng; Guobing, Teng; Houdong, Guo

2016-05-01

A novel trench field stop (FS) insulated gate bipolar transistor (IGBT) with a trench shorted anode (TSA) is proposed. By introducing a trench shorted anode, the TSA-FS-IGBT can obviously improve the breakdown voltage. As the simulation results show, the breakdown voltage is improved by a factor of 19.5% with a lower leakage current compared with the conventional FS-IGBT. The turn off time of the proposed structure is 50% lower than the conventional one with less than 9% voltage drop increased at a current density of 150 A/cm2. Additionally, there is no snapback observed. As a result, the TSA-FS-IGBT has a better trade-off relationship between the turn off loss and forward drop. Project supported by the National Natural Science Foundation of China (No. 61274080) and the Postdoctoral Science Foundation of China (No. 2013M541585).

SU-C-17A-02: Sirius MRI Markers for Prostate Post-Implant Assessment: MR Protocol Development

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lim, T; Wang, J; Kudchadker, R

Purpose: Currently, CT is used to visualize prostate brachytherapy sources, at the expense of accurate structure contouring. MRI is superior to CT for anatomical delineation, but the sources appear as voids on MRI images. Previously we have developed Sirius MRI markers (C4 Imaging) to replace spacers to assist source localization on MRI images. Here we develop an MRI pulse sequence protocol that enhances the signal of these markers to enable MRI-only post-implant prostate dosimetric analysis. Methods: To simulate a clinical scenario, a CIRS multi-modality prostate phantom was implanted with 66 markers and 86 sources. The implanted phantom was imaged onmore » both 1.5T and 3.0T GE scanners under various conditions, different pulse sequences (2D fast spin echo [FSE], 3D balanced steadystate free precession [bSSFP] and 3D fast spoiled gradient echo [FSPGR]), as well as varying amount of padding to simulate various patient sizes and associated signal fall-off from the surface coil elements. Standard FSE sequences from the current clinical protocols were also evaluated. Marker visibility, marker size, intra-marker distance, total scan time and artifacts were evaluated for various combinations of echo time, repetition time, flip angle, number of excitations, bandwidth, slice thickness and spacing, fieldof- view, frequency/phase encoding steps and frequency direction. Results: We have developed a 3D FSPGR pulse sequence that enhances marker signal and ensures the integrity of the marker shape while maintaining reasonable scan time. For patients contraindicated for 3.0T, we have also developed a similar sequence for 1.5T scanners. Signal fall-off with distance from prostate to coil can be compensated mainly by decreasing bandwidth. The markers are not visible using standard FSE sequences. FSPGR sequences are more robust for consistent marker visualization as compared to bSSFP sequences. Conclusion: The developed MRI pulse sequence protocol for Sirius MRI markers assists source localization to enable MRIonly post-implant prostate dosimetric analysis. S.J. Frank is a co-founder of C4 Imaging (manufactures the MRI markers)« less

33 CFR 334.10 - Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area. 334.10 Section 334.10 Navigation and Navigable Waters CORPS... REGULATIONS § 334.10 Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area. (a) The danger...

33 CFR 334.10 - Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area. 334.10 Section 334.10 Navigation and Navigable Waters CORPS... REGULATIONS § 334.10 Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area. (a) The danger...

33 CFR 334.20 - Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area. 334.20 Section 334.20 Navigation and Navigable Waters... REGULATIONS § 334.20 Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area. (a) The...

33 CFR 334.20 - Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area. 334.20 Section 334.20 Navigation and Navigable Waters... REGULATIONS § 334.20 Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area. (a) The...

33 CFR 334.10 - Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area. 334.10 Section 334.10 Navigation and Navigable Waters CORPS... REGULATIONS § 334.10 Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area. (a) The danger...

33 CFR 334.20 - Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area. 334.20 Section 334.20 Navigation and Navigable Waters... REGULATIONS § 334.20 Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area. (a) The...

33 CFR 334.20 - Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area. 334.20 Section 334.20 Navigation and Navigable Waters... REGULATIONS § 334.20 Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area. (a) The...

33 CFR 334.20 - Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area. 334.20 Section 334.20 Navigation and Navigable Waters... REGULATIONS § 334.20 Gulf of Maine off Cape Small, Maine; naval aircraft practice mining range area. (a) The...

33 CFR 334.10 - Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area. 334.10 Section 334.10 Navigation and Navigable Waters CORPS... REGULATIONS § 334.10 Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area. (a) The danger...

A millisecond pulsar in an extremely wide binary system

NASA Astrophysics Data System (ADS)

Bassa, C. G.; Janssen, G. H.; Stappers, B. W.; Tauris, T. M.; Wevers, T.; Jonker, P. G.; Lentati, L.; Verbiest, J. P. W.; Desvignes, G.; Graikou, E.; Guillemot, L.; Freire, P. C. C.; Lazarus, P.; Caballero, R. N.; Champion, D. J.; Cognard, I.; Jessner, A.; Jordan, C.; Karuppusamy, R.; Kramer, M.; Lazaridis, K.; Lee, K. J.; Liu, K.; Lyne, A. G.; McKee, J.; Osłowski, S.; Perrodin, D.; Sanidas, S.; Shaifullah, G.; Smits, R.; Theureau, G.; Tiburzi, C.; Zhu, W. W.

2016-08-01

We report on 22 yr of radio timing observations of the millisecond pulsar J1024-0719 by the telescopes participating in the European Pulsar Timing Array (EPTA). These observations reveal a significant second derivative of the pulsar spin frequency and confirm the discrepancy between the parallax and Shklovskii distances that has been reported earlier. We also present optical astrometry, photometry and spectroscopy of 2MASS J10243869-0719190. We find that it is a low-metallicity main-sequence star (K7V spectral type, [M/H] = -1.0, Teff = 4050 ± 50 K) and that its position, proper motion and distance are consistent with those of PSR J1024-0719. We conclude that PSR J1024-0719 and 2MASS J10243869-0719190 form a common proper motion pair and are gravitationally bound. The gravitational interaction between the main-sequence star and the pulsar accounts for the spin frequency derivatives, which in turn resolves the distance discrepancy. Our observations suggest that the pulsar and main-sequence star are in an extremely wide (Pb > 200 yr) orbit. Combining the radial velocity of the companion and proper motion of the pulsar, we find that the binary system has a high spatial velocity of 384 ± 45 km s-1 with respect to the local standard of rest and has a Galactic orbit consistent with halo objects. Since the observed main-sequence companion star cannot have recycled the pulsar to millisecond spin periods, an exotic formation scenario is required. We demonstrate that this extremely wide-orbit binary could have evolved from a triple system that underwent an asymmetric supernova explosion, though find that significant fine-tuning during the explosion is required. Finally, we discuss the implications of the long period orbit on the timing stability of PSR J1024-0719 in light of its inclusion in pulsar timing arrays.

Understanding the optics to aid microscopy image segmentation.

PubMed

Yin, Zhaozheng; Li, Kang; Kanade, Takeo; Chen, Mei

2010-01-01

Image segmentation is essential for many automated microscopy image analysis systems. Rather than treating microscopy images as general natural images and rushing into the image processing warehouse for solutions, we propose to study a microscope's optical properties to model its image formation process first using phase contrast microscopy as an exemplar. It turns out that the phase contrast imaging system can be relatively well explained by a linear imaging model. Using this model, we formulate a quadratic optimization function with sparseness and smoothness regularizations to restore the "authentic" phase contrast images that directly correspond to specimen's optical path length without phase contrast artifacts such as halo and shade-off. With artifacts removed, high quality segmentation can be achieved by simply thresholding the restored images. The imaging model and restoration method are quantitatively evaluated on two sequences with thousands of cells captured over several days.

Lunar module voice recorder

NASA Technical Reports Server (NTRS)

1974-01-01

A feasibility unit suitable for use as a voice recorder on the space shuttle was developed. A modification, development, and test program is described. A LM-DSEA recorder was modified to achieve the following goals: (1) redesign case to allow in-flight cartridge change; (2) time code change from LM code to IRIG-B 100 pps code; (3) delete cold plate requirements (also requires deletion of long-term thermal vacuum operation at 0.00001 MMHg); (4) implement track sequence reset during cartridge change; (5) reduce record time per cartridge because of unavailability of LM thin-base tape; and (6) add an internal Vox key circuit to turn on/off transport and electronics with voice data input signal. The recorder was tested at both the LM and shuttle vibration levels. The modified recorder achieved the same level of flutter during vibration as the DSEA recorder prior to modification. Several improvements were made over the specification requirements. The high manufacturing cost is discussed.

Improved tolerance to off-resonance in spectral-spatial EPI of hyperpolarized [1-13 C]pyruvate and metabolites.

PubMed

Lau, Justin Y C; Geraghty, Benjamin J; Chen, Albert P; Cunningham, Charles H

2018-09-01

For 13 C echo-planar imaging (EPI) with spectral-spatial excitation, main field inhomogeneity can result in reduced flip angle and spatial artifacts. A hybrid time-resolved pulse sequence, multi-echo spectral-spatial EPI, is proposed combining broader spectral-spatial passbands for greater off-resonance tolerance with a multi-echo acquisition to separate signals from potentially co-excited resonances. The performance of the imaging sequence and the reconstruction pipeline were evaluated for 1 H imaging using a series of increasingly dilute 1,4-dioxane solutions and for 13 C imaging using an ethylene glycol phantom. Hyperpolarized [1- 13 C]pyruvate was administered to two healthy rats. Multi-echo data of the rat kidneys were acquired to test realistic cases of off-resonance. Analysis of separated images of water and 1,4-dioxane following multi-echo signal decomposition showed water-to-dioxane 1 H signal ratios that were in agreement with the independent measurements by 1 H spectroscopy for all four concentrations of 1,4-dioxane. The 13 C signal ratio of two co-excited resonances of ethylene glycol was accurately recovered after correction for the spectral profile of the redesigned spectral-spatial pulse. In vivo, successful separation of lactate and pyruvate-hydrate signals was achieved for all except the early time points during which signal variations exceeded the temporal resolution of the multi-echo acquisition. Improved tolerance to off-resonance in the new 13 C data acquisition pipeline was demonstrated in vitro and in vivo. Magn Reson Med 80:925-934, 2018. © 2018 International Society for Magnetic Resonance in Medicine. © 2018 International Society for Magnetic Resonance in Medicine.

78 FR 11271 - Reports, Forms, and Record keeping Requirements

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-15

... following proposed collections of information: Title: Air Bag Deactivation. OMB Control Number: 2127-0588.... Abstract: If a private individual or lessee wants to install an air bag on-off switch to turn-off either or both frontal air bags, they must complete Form OMB 2127-0588 to certify certain statements regarding...

78 FR 38095 - Request for Comments of a Previously Approved Information Collection

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-25

.... Mrs. Ball's telephone number is (202) 366-2245. SUPPLEMENTARY INFORMATION: Title: Air Bag Deactivation... vehicle dealers, repair business. Abstract: If a private individual or lessee wants to install an air bag on-off switch to turn-off either or both frontal air bags, they must complete Form OMB 2127-0588 to...

Reduced Performance in Balance, Walking and Turning Tasks is Associated with Increased Neck Tone in Parkinson's Disease

PubMed Central

Franzén, Erika; Paquette, Caroline; Gurfinkel, Victor S; Cordo, Paul J; Nutt, John G; Horak, Fay B

2009-01-01

Rigidity or hypertonicity is a cardinal symptom of Parkinson's disease (PD). We hypothesized that hypertonicity of the body axis affects functional performance of tasks involving balance, walking and turning. The magnitude of axial postural tone in the neck, trunk and hip segments of 15 subjects with PD (both ON and OFF levodopa) and 15 control subjects was quantified during unsupported standing in an axial twisting device in our laboratory as resistance to torsional rotation. Subjects also performed six functional tests (walking in a figure of eight [Figure of Eight], Timed Up & Go, Berg Balance Scale, supine rolling task [rollover], Functional Reach, and standing 360-deg turn-in-place) in the ON and OFF state. Results showed that PD subjects had increased tone throughout the axis compared to control subjects (p=0.008) and that this increase was most prominent in the neck. In PD subjects, axial tone was related to functional performance, but most strongly for tone at the neck and accounted for an especially large portion of the variability in the performance of the Figure of Eight test (rOFF=0.68 and rON=0.74, p<0.05) and the Rollover test (rOFF=0.67and rON=0.55, p<0.05). Our results suggest that neck tone plays a significant role in functional mobility and that abnormally high postural tone may be an important contributor to balance and mobility disorders in individuals with PD. PMID:19573528

GapFiller: a de novo assembly approach to fill the gap within paired reads

PubMed Central

2012-01-01

Background Next Generation Sequencing technologies are able to provide high genome coverages at a relatively low cost. However, due to limited reads' length (from 30 bp up to 200 bp), specific bioinformatics problems have become even more difficult to solve. De novo assembly with short reads, for example, is more complicated at least for two reasons: first, the overall amount of "noisy" data to cope with increased and, second, as the reads' length decreases the number of unsolvable repeats grows. Our work's aim is to go at the root of the problem by providing a pre-processing tool capable to produce (in-silico) longer and highly accurate sequences from a collection of Next Generation Sequencing reads. Results In this paper a seed-and-extend local assembler is presented. The kernel algorithm is a loop that, starting from a read used as seed, keeps extending it using heuristics whose main goal is to produce a collection of error-free and longer sequences. In particular, GapFiller carefully detects reliable overlaps and operates clustering similar reads in order to reconstruct the missing part between the two ends of the same insert. Our tool's output has been validated on 24 experiments using both simulated and real paired reads datasets. The output sequences are declared correct when the seed-mate is found. In the experiments performed, GapFiller was able to extend high percentages of the processed seeds and find their mates, with a false positives rate that turned out to be nearly negligible. Conclusions GapFiller, starting from a sufficiently high short reads coverage, is able to produce high coverages of accurate longer sequences (from 300 bp up to 3500 bp). The procedure to perform safe extensions, together with the mate-found check, turned out to be a powerful criterion to guarantee contigs' correctness. GapFiller has further potential, as it could be applied in a number of different scenarios, including the post-processing validation of insertions/deletions detection pipelines, pre-processing routines on datasets for de novo assembly pipelines, or in any hierarchical approach designed to assemble, analyse or validate pools of sequences. PMID:23095524

Structure of a rare non-standard sequence k-turn bound by L7Ae protein

PubMed Central

Huang, Lin; Lilley, David M.J.

2014-01-01

Kt-23 from Thelohania solenopsae is a rare RNA kink turn (k-turn) where an adenine replaces the normal guanine at the 2n position. L7Ae is a member of a strongly conserved family of proteins that bind a range of k-turn structures in the ribosome, box C/D and H/ACA small nucleolar RNAs and U4 small nuclear RNA. We have solved the crystal structure of T. solenopsae Kt-23 RNA bound to Archeoglobus fulgidus L7Ae protein at a resolution of 2.95 Å. The protein binds in the major groove displayed on the outer face of the k-turn, in a manner similar to complexes with standard k-turn structures. The k-turn adopts a standard N3 class conformation, with a single hydrogen bond from A2b N6 to A2n N3. This contrasts with the structure of the same sequence located in the SAM-I riboswitch, where it adopts an N1 structure, showing the inherent plasticity of k-turn structure. This potentially can affect any tertiary interactions in which the RNA participates. PMID:24482444

Selective detection of trace nitroaromatic, nitramine, and nitrate ester explosive residues using a three-step fluorimetric sensing process: a tandem turn-off, turn-on sensor.

PubMed

Sanchez, Jason C; Toal, Sarah J; Wang, Zheng; Dugan, Regina E; Trogler, William C

2007-11-01

Detection of trace quantities of explosive residues plays a key role in military, civilian, and counter-terrorism applications. To advance explosives sensor technology, current methods will need to become cheaper and portable while maintaining sensitivity and selectivity. The detection of common explosives including trinitrotoluene (TNT), cyclotrimethylenetrinitramine, cyclotetramethylene-tetranitramine, pentaerythritol tetranitrate, 2,4,6-trinitrophenyl-N-methylnitramine, and trinitroglycerin may be carried out using a three-step process combining "turn-off" and "turn-on" fluorimetric sensing. This process first detects nitroaromatic explosives by their quenching of green luminescence of polymetalloles (lambda em approximately 400-510 nm). The second step places down a thin film of 2,3-diaminonaphthalene (DAN) while "erasing" the polymetallole luminescence. The final step completes the reaction of the nitramines and/or nitrate esters with DAN resulting in the formation of a blue luminescent traizole complex (lambda(em) = 450 nm) providing a "turn-on" response for nitramine and nitrate ester-based explosives. Detection limits as low as 2 ng are observed. Solid-state detection of production line explosives demonstrates the applicability of this method to real world situations. This method offers a sensitive and selective detection process for a diverse group of the most common high explosives used in military and terrorist applications today.

Propensities of peptides containing the Asn-Gly segment to form β-turn and β-hairpin structures.

PubMed

Kang, Young Kee; Yoo, In Kee

2016-09-01

The propensities of peptides that contain the Asn-Gly segment to form β-turn and β-hairpin structures were explored using the density functional methods and the implicit solvation model in CH2 Cl2 and water. The populations of preferred β-turn structures varied depending on the sequence and solvent polarity. In solution, β-hairpin structures with βI' turn motifs were most preferred for the heptapeptides containing the Asn-Gly segment regardless of the sequence of the strands. These preferences in solution are consistent with the corresponding X-ray structures. The sequence, H-bond strengths, solvent polarity, and conformational flexibility appeared to interact to determine the preferred β-hairpin structure of each heptapeptide, although the β-turn segments played a role in promoting the formation of β-hairpin structures and the β-hairpin propensity varied. In the heptapeptides containing the Asn-Gly segment, the β-hairpin formation was enthalpically favored and entropically disfavored at 25°C in water. The calculated results for β-turns and β-hairpins containing the Asn-Gly segment imply that these structural preferences may be useful for the design of bioactive macrocyclic peptides containing β-hairpin mimics and the design of binding epitopes for protein-protein and protein-nucleic acid recognitions. © 2016 Wiley Periodicals, Inc. Biopolymers 105: 653-664, 2016. © 2016 Wiley Periodicals, Inc.

Development and clinical application of a computer-aided real-time feedback system for detecting in-bed physical activities.

PubMed

Lu, Liang-Hsuan; Chiang, Shang-Lin; Wei, Shun-Hwa; Lin, Chueh-Ho; Sung, Wen-Hsu

2017-08-01

Being bedridden long-term can cause deterioration in patients' physiological function and performance, limiting daily activities and increasing the incidence of falls and other accidental injuries. Little research has been carried out in designing effective detecting systems to monitor the posture and status of bedridden patients and to provide accurate real-time feedback on posture. The purposes of this research were to develop a computer-aided system for real-time detection of physical activities in bed and to validate the system's validity and test-retest reliability in determining eight postures: motion leftward/rightward, turning over leftward/rightward, getting up leftward/rightward, and getting off the bed leftward/rightward. The in-bed physical activity detecting system consists mainly of a clinical sickbed, signal amplifier, a data acquisition (DAQ) system, and operating software for computing and determining postural changes associated with four load cell sensing components. Thirty healthy subjects (15 males and 15 females, mean age = 27.8 ± 5.3 years) participated in the study. All subjects were asked to execute eight in-bed activities in a random order and to participate in an evaluation of the test-retest reliability of the results 14 days later. Spearman's rank correlation coefficient was used to compare the system's determinations of postural states with researchers' recordings of postural changes. The test-retest reliability of the system's ability to determine postures was analyzed using the interclass correlation coefficient ICC(3,1). The system was found to exhibit high validity and accuracy (r = 0.928, p < 0.001; accuracy rate: 87.9%) in determining in-bed displacement, turning over, sitting up, and getting off the bed. The system was particularly accurate in detecting motion rightward (90%), turning over leftward (83%), sitting up leftward or rightward (87-93%), and getting off the bed (100%). The test-retest reliability ICC(3,1) value was 0.968 (p < 0.001). The system developed in this study exhibits satisfactory validity and reliability in detecting changes in-bed body postures and can be beneficial in assisting caregivers and clinical nursing staff in detecting the in-bed physical activities of bedridden patients and in developing fall prevention warning systems. Copyright © 2017 Elsevier B.V. All rights reserved.

EDIN design study alternate space shuttle booster replacement concepts. Volume 2: Design simulation results

NASA Technical Reports Server (NTRS)

Demakes, P. T.; Hirsch, G. N.; Stewart, W. A.; Glatt, C. R.

1976-01-01

Historical weight estimating relationships were developed for the liquid rocket booster (LRB) using Saturn technology, and modified as required to support the EDIN05 study. Mission performance was computed using February 1975 shuttle configuration groundrules to allow reasonable comparison of the existing shuttle with the EDIN05 designs. The launch trajectory was constrained to pass through both the RTLS/AOA and main engine cut-off points. Performance analysis was based on a point design trajectory model which optimized initial tilt rate and exo-atmospheric pitch profile. A gravity turn was employed during the boost phase in place of the shuttle angle-of-attack profile. Engine throttling add/or shutdown was used to constrain dynamic pressure and/or longitudinal acceleration where necessary.

Research on Electromagnetic Force Distribution and Vibration Performance of A Novel 10/4 Switched Reluctance Motor

NASA Astrophysics Data System (ADS)

Fu, Ziyu; Wang, Xinyu; Cao, Cheng; Liu, Meng; Wang, Kangxi

2017-06-01

Radial electromagnetic force is one of the main reasons causing the vibration and noise of the switched reluctance motor. Based on this, the novel structure of 10/4 pole switched reluctance motor is proposed, which increases the air gap flux and electromagnetic torque by increasing the number of stator poles. In addition, the excitation current of the stator winding is reduced by early turn-off angle. Through the finite element modelling analysis, the results show the superiority of the new type of switched reluctance motor. In the end, the vibration characteristics of the conventional motor and the new motor are compared and analysed, and the effect of the structure of this new type of switched reluctance motor is verified.

Resonant power supplies for a rapid-cycling accelerator

NASA Astrophysics Data System (ADS)

Karady, G.; Thiessen, H. A.; Schneider, E. J.

1988-10-01

A resonant power supply has been proposed as an efficient power supply for a future 60-GeV, Kaon-producing accelerator. The engineering design of the electric system of the main-ring power supplies is described. It is shown that the resonant power supply can be built with standard commercially available components. The most critical component is the bypass switch, which requires gate-turn off thyristors, connected in parallel. Standard metal-clad switchgear can be used for the AC system. The resonant power supplies can be fed directly from the 115-kV utility network, but the resonance power supplies draw pulse loads from the utility network. This pulse may produce disturbances. AC filter and reactive power compensation is needed for economical operation.

Analysis of proton irradiated n- and p-type strained FinFETs at low temperatures down to 100 K

NASA Astrophysics Data System (ADS)

Vicentis Caparroz, Luis Felipe; Mendes Bordallo, Caio Cesar; Martino, Joao Antonio; Simoen, Eddy; Claeys, Cor; Ghedini Der Agopian, Paula

2018-06-01

This paper studies the main low temperature electrical parameters of SOI n- and p-type FinFETs, standard and strained devices, submitted to proton irradiation. The study covers the range from room temperature down to 100 K, focusing on the threshold voltage (VTH), subthreshold swing (SS), the Early voltage VEA, transistor efficiency and the intrinsic gain voltage (AV) for 3 different channel widths. The p-channel devices showed a greater immunity to radiation than the n-channel ones, when considering the basic parameters thanks to the back conduction turn-off tendency, while from the analog parameters point of view, both transistor types presented a similar response to proton radiation at strong inversion.

Improved first-pass spiral myocardial perfusion imaging with variable density trajectories.

PubMed

Salerno, Michael; Sica, Christopher; Kramer, Christopher M; Meyer, Craig H

2013-11-01

To develop and evaluate variable-density spiral first-pass perfusion pulse sequences for improved efficiency and off-resonance performance and to demonstrate the utility of an apodizing density compensation function (DCF) to improve signal-to-noise ratio (SNR) and reduce dark-rim artifact caused by cardiac motion and Gibbs Ringing. Three variable density spiral trajectories were designed, simulated, and evaluated in 18 normal subjects, and in eight patients with cardiac pathology on a 1.5T scanner. By using a DCF, which intentionally apodizes the k-space data, the sidelobe amplitude of the theoretical point spread function (PSF) is reduced by 68%, with only a 13% increase in the full-width at half-maximum of the main-lobe when compared with the same data corrected with a conventional variable-density DCF, and has an 8% higher resolution than a uniform density spiral with the same number of interleaves and readout duration. Furthermore, this strategy results in a greater than 60% increase in measured SNR when compared with the same variable-density spiral data corrected with a conventional DCF (P < 0.01). Perfusion defects could be clearly visualized with minimal off-resonance and dark-rim artifacts. Variable-density spiral pulse sequences using an apodized DCF produce high-quality first-pass perfusion images with minimal dark-rim and off-resonance artifacts, high SNR and contrast-to-noise ratio, and good delineation of resting perfusion abnormalities. Copyright © 2012 Wiley Periodicals, Inc.

Sequence-specific, nanomolar peptide binding via cucurbit[8]uril-induced folding and inclusion of neighboring side chains.

PubMed

Smith, Lauren C; Leach, David G; Blaylock, Brittney E; Ali, Omar A; Urbach, Adam R

2015-03-18

This paper describes the molecular recognition of the tripeptide Tyr-Leu-Ala by the synthetic receptor cucurbit[8]uril (Q8) in aqueous buffer with nanomolar affinity and exceptional specificity. This combination of characteristics, which also applies to antibodies, is desirable for applications in biochemistry and biotechnology but has eluded supramolecular chemists for decades. Building on prior knowledge that Q8 binds to peptides with N-terminal aromatic residues, a library screen of 105 peptides was designed to test the effects of residues adjacent to N-terminal Trp, Phe, or Tyr. The screen used tetramethylbenzobis(imidazolium) (MBBI) as a fluorescent indicator and resulted in the unexpected discovery that MBBI can serve not only as a turn-off sensor via the simultaneous inclusion of a Trp residue but also as a turn-on sensor via the competitive displacement of MBBI upon binding of Phe- or Tyr-terminated peptides. The unusual fluorescence response of the Tyr series prompted further investigation by (1)H NMR spectroscopy, electrospray ionization mass spectrometry, and isothermal titration calorimetry. From these studies, a novel binding motif was discovered in which only 1 equiv of peptide binds to Q8, and the side chains of both the N-terminal Tyr residue and its immediate neighbor bind within the Q8 cavity. For the peptide Tyr-Leu-Ala, the equilibrium dissociation constant value is 7.2 nM, whereas that of its sequence isomer Tyr-Ala-Leu is 34 μM. The high stability, recyclability, and low cost of Q8 combined with the straightforward incorporation of Tyr-Leu-Ala into recombinant proteins should make this system attractive for the development of biological applications.

40 CFR 63.463 - Batch vapor and in-line cleaning machine standards.

Code of Federal Regulations, 2012 CFR

2012-07-01

... be turned off and the solvent vapor layer allowed to collapse before the primary condenser is turned... separate both the continuous web part feed reel and take-up reel from the room atmosphere if the doors are... from the room atmosphere if the doors are checked according to the requirements of paragraph (e)(2)(iii...

40 CFR 63.463 - Batch vapor and in-line cleaning machine standards.

Code of Federal Regulations, 2013 CFR

2013-07-01

... be turned off and the solvent vapor layer allowed to collapse before the primary condenser is turned... separate both the continuous web part feed reel and take-up reel from the room atmosphere if the doors are... from the room atmosphere if the doors are checked according to the requirements of paragraph (e)(2)(iii...

JPRS Report, Near East and South Asia.

DTIC Science & Technology

1991-06-24

a brackish, dark blue river that gives off cafes that have turned over a new leaf these past few nauseating odors amidst the otherwise very well...tended years. greens. "All it took was for one cafe in town to turn over a new Professionals cite the same problems that are damaging leaf for all the...other cafes to follow it in turn and tackle the image of our tourist product. the job one after another." Moreover, the height of the deterioration of

MOSFET Power Controller

NASA Technical Reports Server (NTRS)

Mitchell, J.; Jones, K.

1986-01-01

High current and voltage controlled remotely. Remote Power Conroller includes two series-connected banks of parallel-connected MOSFET's to withstand high current and voltage. Voltage sharing between switch banks, low-impedance, gate-drive circuits used. Provided controlled range for turn on. Individually trimmable to insure simultaneous switching within few nanoseconds during both turn on and turn off. Control circuit for each switch bank and over-current trip circuit float independently and supplied power via transformer T1 from inverter. Control of floating stages by optocouplers.

Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+ between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity

PubMed Central

Zhao, Liu; Miao, Yanqing; Liu, Chunye; Zhang, Chenxiao

2016-01-01

The detection of pyrophosphatase (PPase) activity is of great significance in diagnosing diseases and understanding the function of PPase-related biological events. This study constructed a turn off-on-off fluorescent system for PPase activity assay based on PPase-regulated competitive coordination of Cu2+ between a water-soluble fluorescent probe 6,7-dihydroxycoumarin (DHC) and pyrophosphate (PPi). The probe DHC can coordinate with Cu2+ and consequently display on-off type fluorescence response. Furthermore, the in situ formed nonfluorescent Cu2+-DHC complex can act as an effective off-on type fluorescent probe for sensing PPi due to the higher coordination reactivity between Cu2+ and PPi than that between Cu2+ and DHC. The subsequent addition of PPase to the mixture containing Cu2+, DHC, and PPi leads to the fluorescence requenching of the system again (an off state) because PPase catalyzes the hydrolysis of PPi into orthophosphate in the reaction system. Under the optimum conditions, the decrease of the fluorescence intensity of DHC-Cu2+-PPi system was linear with the increase of the PPase activity in the range from 0.1 to 0.3 U. The detection limit was down to 0.028 U PPase (S/N = 3). Moreover, the as-established system was also applied to evaluate PPase inhibitor. This study offers a simple yet effective method for the detection of PPase activity. PMID:27766179

Constant power speed range extension of surface mounted PM motors

DOEpatents

Lawler, Jack Steward; Bailey, John Milton

2001-01-01

A circuit and method for controlling a rotating machine (11) in the constant horsepower range above base speed uses an inverter (15) having SCR's (T1-T6) connected in series with the primary commutation switches (Q1-Q6) to control turn off of the primary commutation switches and to protect the primary commutation switches from faults. The primary commutation switches (Q1-Q6) are controlled by a controller (14), to fire in advance or after a time when the back emf equals the applied voltage, and then to turn off after a precise dwell time, such that suitable power is developed at speeds up to at least six times base speed.

Genotypic and phenotypic evaluation of off-type grasses in hybrid Bermudagrass [Cynodon dactylon (L.) Pers. x C. transvaalensis Burtt-Davy] putting greens using genotyping-by-sequencing and morphological characterization.

PubMed

Reasor, Eric H; Brosnan, James T; Staton, Margaret E; Lane, Thomas; Trigiano, Robert N; Wadl, Phillip A; Conner, Joann A; Schwartz, Brian M

2018-01-01

Interspecific hybrid bermudagrass [ Cynodon dactylon (L.) Pers. x C. transvaalensis Burtt-Davy] is one of the most widely used grasses on golf courses, with cultivars derived from 'Tifgreen' or 'Tifdwarf' particularly used for putting greens. Many bermudagrass cultivars established for putting greens can be genetically unstable and lead to the occurrence of undesirable off-type grasses that vary in phenotype. The objective of this research was to genetically and phenotypically differentiate off-type grasses and hybrid cultivars. Beginning in 2013, off-type and desirable hybrid bermudagrass samples were collected from golf course putting greens in the southeastern United States and genetically and phenotypically characterized using genotyping-by-sequencing and morphology. Genotyping-by-sequencing determined that 11% (5) of off-type and desirable samples from putting greens were genetically divergent from standard cultivars such as Champion, MiniVerde, Tifdwarf, TifEagle, and Tifgreen. In addition, genotyping-by-sequencing was unable to genetically distinguish all standard cultivars from one another due to their similar origin and clonal propagation; however, over 90,000 potentially informative nucleotide variants were identified among the triploid hybrid cultivars. Although few genetic differences were found in this research, samples harvested from golf course putting greens had variable morphology and were clustered into three distinct phenotypic groups. The majority of off-type grasses in hybrid bermudagrass putting greens were genetically similar with variable morphological traits. Off-type grasses within golf course putting greens have the potential to compromise putting surface functionality and aesthetics.

Series resonant converter with auxiliary winding turns: analysis, design and implementation

NASA Astrophysics Data System (ADS)

Lin, Bor-Ren

2018-05-01

Conventional series resonant converters have researched and applied for high-efficiency power units due to the benefit of its low switching losses. The main problems of series resonant converters are wide frequency variation and high circulating current. Thus, resonant converter is limited at narrow input voltage range and large input capacitor is normally adopted in commercial power units to provide the minimum hold-up time requirement when AC power is off. To overcome these problems, the resonant converter with auxiliary secondary windings are presented in this paper to achieve high voltage gain at low input voltage case such as hold-up time duration when utility power is off. Since the high voltage gain is used at low input voltage cased, the frequency variation of the proposed converter compared to the conventional resonant converter is reduced. Compared to conventional resonant converter, the hold-up time in the proposed converter is more than 40ms. The larger magnetising inductance of transformer is used to reduce the circulating current losses. Finally, a laboratory prototype is constructed and experiments are provided to verify the converter performance.

Roles of beta-turns in protein folding: from peptide models to protein engineering.

PubMed

Marcelino, Anna Marie C; Gierasch, Lila M

2008-05-01

Reverse turns are a major class of protein secondary structure; they represent sites of chain reversal and thus sites where the globular character of a protein is created. It has been speculated for many years that turns may nucleate the formation of structure in protein folding, as their propensity to occur will favor the approximation of their flanking regions and their general tendency to be hydrophilic will favor their disposition at the solvent-accessible surface. Reverse turns are local features, and it is therefore not surprising that their structural properties have been extensively studied using peptide models. In this article, we review research on peptide models of turns to test the hypothesis that the propensities of turns to form in short peptides will relate to the roles of corresponding sequences in protein folding. Turns with significant stability as isolated entities should actively promote the folding of a protein, and by contrast, turn sequences that merely allow the chain to adopt conformations required for chain reversal are predicted to be passive in the folding mechanism. We discuss results of protein engineering studies of the roles of turn residues in folding mechanisms. Factors that correlate with the importance of turns in folding indeed include their intrinsic stability, as well as their topological context and their participation in hydrophobic networks within the protein's structure.

Roles of β-Turns in Protein Folding: From Peptide Models to Protein Engineering

PubMed Central

Marcelino, Anna Marie C.; Gierasch, Lila M.

2010-01-01

Reverse turns are a major class of protein secondary structure; they represent sites of chain reversal and thus sites where the globular character of a protein is created. It has been speculated for many years that turns may nucleate the formation of structure in protein folding, as their propensity to occur will favor the approximation of their flanking regions and their general tendency to be hydrophilic will favor their disposition at the solvent-accessible surface. Reverse turns are local features, and it is therefore not surprising that their structural properties have been extensively studied using peptide models. In this article, we review research on peptide models of turns to test the hypothesis that the propensities of turns to form in short peptides will relate to the roles of corresponding sequences in protein folding. Turns with significant stability as isolated entities should actively promote the folding of a protein, and by contrast, turn sequences that merely allow the chain to adopt conformations required for chain reversal are predicted to be passive in the folding mechanism. We discuss results of protein engineering studies of the roles of turn residues in folding mechanisms. Factors that correlate with the importance of turns in folding indeed include their intrinsic stability, as well as their topological context and their participation in hydrophobic networks within the protein’s structure. PMID:18275088

Actin-based vesicular transport in the first 20 min after dusk is crucial for daily rhabdom synthesis in the compound eye of the grapsid crab Hemigrapsus sanguineus.

PubMed

Matsushita, A; Arikawa, K

1997-09-01

In the crab Hemigrapsus sanguineus, maintained under a 12 h:12 h light:dark cycle, the amount of vesicular smooth endoplasmic reticulum (vesicular sER) in the photoreceptor cell body increases after the light is turned off. This paper demonstrates that actin filaments in the photoreceptor cell body are involved in the transport of vesicular sER towards the rhabdom. To specify the time of actin contribution to rhabdom synthesis, we disrupted the organization of actin filaments in the cell body with cytochalasin D at various time around dusk. We then measured the rhabdom size and also examined the ultrastructure of the photoreceptor cell body 3 h after extinguishing the light. When cytochalasin D was applied from either 1 h before or immediately after extinguishing the light, the rhabdom size did not increase, whereas vesicular sER accumulated in the cell body. In contrast, cytochalasin D applied to the eyes from 20 min after turning the light off did not inhibit rhabdom synthesis. These results indicate that the first 20 min after the light is turned off is particularly important for the transport of vesicular sER towards the rhabdom by the cell body actin filaments.

Upper-surface-blowing flow-turning performance

NASA Technical Reports Server (NTRS)

Sleeman, W. C., Jr.; Phelps, A. E., III

1976-01-01

Jet exhaust flow-turning characteristics were determined for systematic variations in upper-surface blowing exhaust nozzles and trailing-edge flap configuration variables from experimental wind-off (static) flow studies. For conditions with parallel flow exhausting from the nozzle, jet height (as indicated by nozzle exit height) and flap radius were found to be the most important parameters relating to flow turning. Nonparallel flow from the nozzle, as obtained from an internal roof angle and/or side spread angle, had a large favorable effect on flow turning. Comparisons made between static turning results and wind tunnel aerodynamic studies of identical configurations indicated that static flow-turning results can be indicative of wind-on powered lift performance for both good and poor nozzle-flap combinations but, for marginal designs, can lead to overly optimistic assessment of powered lift potential.

Navigating Turn-Taking and Conversational Repair in an Online Synchronous Course

ERIC Educational Resources Information Center

Earnshaw, Yvonne

2017-01-01

In face-to-face conversations, speaker transitions (or hand-offs) are typically seamless. In computer-mediated communication settings, speaker hand-offs can be a bit more challenging. This paper presents the results of a study of audio communication problems that occur in an online synchronous course, and how, and by whom, those problems are…

75 FR 19453 - Reports, Forms and Record Keeping Requirements; Agency Information Collection Activity Under OMB...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-14

... Administration Title: Air Bag Deactivation. OMB Number: 2127-0588. Type of Request: Extension of a currently approved information collection. Abstract: If a private individual or lessee wants to install an air bag on-off switch to turn-off either or both frontal air bags, they must complete Form OMB 2127-0588 to...

Plasma line overshoot Observations during the July 2017 HF campaign at the Arecibo Observatory

NASA Astrophysics Data System (ADS)

Coster, A. J.; Nossa, E.; Kendall, E. A.; Bhatt, A.

2017-12-01

In the 1970's and 80's, enhanced plasma waves were frequently detected by the 430 MHz radar at the Arecibo Observatory within 2 to 8 msec after the HF heater had been turned on (Showen, 1975). This phenomenon is best described as an increase in the return power level of the plasma line by one or two orders of magnitude, followed by a slow decay back to its steady-state value. The formation of short scale striations has frequently been cited as a plausible explanation of the plasma line overshoot. The idea is that as the striations form, they absorb or scatter energy from the HF heater wave. This diminishes the amount of power available to excite the plasma waves detected. Short scale striations are thought to form at the altitude where the frequency of the HF wave matches the upper hybrid resonance - below the altitude of HF reflection. In 1981, the decay of the plasma line overshoot was correlated with the rise of the short scale striations utilizing an HF pulse rate of 4 seconds on/off, with the HF transmitter tuned to 5.1 MHz, and an estimated E.R.P. of 38 MW (Coster et al., 1984). In July 2017, a new series of experiments were performed using the new Arecibo HF facility to reexamine the enhanced plasma line issue, and utilizing the superior diagnostic capabilities available in 2017. These experiments were designed to study possible mechanisms responsible for the formation and decay of the plasma line overshoot. In the experiment, the HF heater was cycled on and off at various sequences including: 8 min on/off, 4 min on/off, and 2 min on/off. The enhanced plasma line was detected throughout the experiment. We will summarize the observations of this experiment, report on the significant findings, and provide suggestions for future experiments.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Krishnamurthy, Malathy; Hennelly, Scott Patrick; Dale, Taraka T.

The most straightforward approach to altering the flux through a particular metabolic step is to increase or decrease the concentration of the enzyme catalyst. Until recently engineering strategies for altering gene expression have focused on transcription control using strong inducible promoters or by using one of several strategies to knock down or knock out a wasteful gene. Recently, synthetic riboregulators have been developed for translational regulation of gene expression. We report a new modular synthetic riboregulator class that has the potential to finely tune protein expression and independently control the concentration of each enzyme in an engineered metabolic pathway. Ourmore » design includes a cis-repressor at the 5’ end of the mRNA that forms a stem-loop helix occluding the ribosome binding site and blocking translation. An activating-RNA, expressed in trans, frees the RBS turning on translation. The overall architecture of the riboregulators is designed using Watson-Crick base-pairing stability followed by directed evolution on a portion of each trans-activator to fine tune translation. We report a cis-repressor that can completely shut off translation of antibiotic resistance reporters and a trans-activator that restores translation. We have shown it is possible to use riboregulators to achieve translational control of gene expression over a wide dynamic range. Using a bioluminescent reporter system, we demonstrated an ON/OFF ratio >300. We have demonstrated that a targeting sequence can be changed to develop riboregulators that can independently regulate translation of many genes with minimal cross-talk. In a SELEX experiment, we demonstrated that by subtly altering the sequence of the trans-activator, it is possible to alter the equilibrium between repressed and activated states and achieve intermediate translational control.« less

Tunable riboregulator switches for post-transcriptional control of gene expression

DOE PAGES

Krishnamurthy, Malathy; Hennelly, Scott Patrick; Dale, Taraka T.; ...

2015-07-13

The most straightforward approach to altering the flux through a particular metabolic step is to increase or decrease the concentration of the enzyme catalyst. Until recently engineering strategies for altering gene expression have focused on transcription control using strong inducible promoters or by using one of several strategies to knock down or knock out a wasteful gene. Recently, synthetic riboregulators have been developed for translational regulation of gene expression. We report a new modular synthetic riboregulator class that has the potential to finely tune protein expression and independently control the concentration of each enzyme in an engineered metabolic pathway. Ourmore » design includes a cis-repressor at the 5’ end of the mRNA that forms a stem-loop helix occluding the ribosome binding site and blocking translation. An activating-RNA, expressed in trans, frees the RBS turning on translation. The overall architecture of the riboregulators is designed using Watson-Crick base-pairing stability followed by directed evolution on a portion of each trans-activator to fine tune translation. We report a cis-repressor that can completely shut off translation of antibiotic resistance reporters and a trans-activator that restores translation. We have shown it is possible to use riboregulators to achieve translational control of gene expression over a wide dynamic range. Using a bioluminescent reporter system, we demonstrated an ON/OFF ratio >300. We have demonstrated that a targeting sequence can be changed to develop riboregulators that can independently regulate translation of many genes with minimal cross-talk. In a SELEX experiment, we demonstrated that by subtly altering the sequence of the trans-activator, it is possible to alter the equilibrium between repressed and activated states and achieve intermediate translational control.« less

Visualizing Key Hinges and a Potential Major Source of Compliance in the Lever Arm of Myosin

DOE Office of Scientific and Technical Information (OSTI.GOV)

J Brown; V Senthil Kumar; E ONeall-Hennessey

2011-12-31

We have determined the 2.3-{angstrom}-resolution crystal structure of a myosin light chain domain, corresponding to one type found in sea scallop catch ('smooth') muscle. This structure reveals hinges that may function in the 'on' and 'off' states of myosin. The molecule adopts two different conformations about the heavy chain 'hook' and regulatory light chain (RLC) helix D. This conformational change results in extended and compressed forms of the lever arm whose lengths differ by 10 {angstrom}. The heavy chain hook and RLC helix D hinges could thus serve as a potential major and localized source of cross-bridge compliance during themore » contractile cycle. In addition, in one of the molecules of the crystal, part of the RLC N-terminal extension is seen in atomic detail and forms a one-turn alpha-helix that interacts with RLC helix D. This extension, whose sequence is highly variable in different myosins, may thus modulate the flexibility of the lever arm. Moreover, the relative proximity of the phosphorylation site to the helix D hinge suggests a potential role for conformational changes about this hinge in the transition between the on and off states of regulated myosins.« less

Visualizing key hinges and a potential major source of compliance in the lever arm of myosin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brown, J.H.; Robinson, H.; Senthil Kumar, V. S.

2011-01-04

We have determined the 2.3-{angstrom}-resolution crystal structure of a myosin light chain domain, corresponding to one type found in sea scallop catch ('smooth') muscle. This structure reveals hinges that may function in the 'on' and 'off' states of myosin. The molecule adopts two different conformations about the heavy chain 'hook' and regulatory light chain (RLC) helix D. This conformational change results in extended and compressed forms of the lever arm whose lengths differ by 10 {angstrom}. The heavy chain hook and RLC helix D hinges could thus serve as a potential major and localized source of cross-bridge compliance during themore » contractile cycle. In addition, in one of the molecules of the crystal, part of the RLC N-terminal extension is seen in atomic detail and forms a one-turn alpha-helix that interacts with RLC helix D. This extension, whose sequence is highly variable in different myosins, may thus modulate the flexibility of the lever arm. Moreover, the relative proximity of the phosphorylation site to the helix D hinge suggests a potential role for conformational changes about this hinge in the transition between the on and off states of regulated myosins.« less

33 CFR 334.10 - Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Gulf of Maine off Seal Island... REGULATIONS § 334.10 Gulf of Maine off Seal Island, Maine; naval aircraft bombing target area. (a) The danger zone. A circular area with a radius of 1.5 nautical miles, having its center just easterly of Seal...

Cut-off Points for Muscle Mass - Not Grip Strength or Gait Speed - Determine Variations in Sarcopenia Prevalence.

PubMed

Masanés, F; Rojano I Luque, X; Salvà, A; Serra-Rexach, J A; Artaza, I; Formiga, F; Cuesta, F; López Soto, A; Ruiz, D; Cruz-Jentoft, A J

2017-01-01

The European Working Group on Sarcopenia in Older People (EWGSOP) has proposed different methods and cut-off points for the three parameters that define sarcopenia: muscle mass, muscle strength and physical performance. Although this facilitates clinical practice, it limits comparability between studies and leads to wide differences in published prevalence rates. The aim of this study was to assess how changes in cut-off points for muscle mass, gait speed and grip strength affected sarcopenia prevalence according to EWGSOP criteria. Cross-sectional analysis of elderly individuals recruited from outpatient clinics (n=298) and nursing homes (n=276). We measured muscle mass, grip strength and gait speed and assessed how changes in cut-off points changed sarcopenia prevalence in both populations. An increase from 5.45 kg/m2 to 6.68 kg/m2 in the muscle mass index for female outpatients and nursing-home residents increased sarcopenia prevalence from 4% to 23% and from 9% to 47%, respectively; for men, for an increase from 7.25 kg/m2 to 8.87 kg/m2, the corresponding increases were from 1% to 22% and from 6% to 41%, respectively. Changes in gait speed and grip strength had a limited impact on sarcopenia prevalence. The cut-off points used for muscle mass affect the reported prevalence rates for sarcopenia and, in turn, affect comparability between studies. The main factors influencing the magnitude of the change are muscle mass index distribution in the population and the absolute value of the cut-off points: the same difference between two references (e.g., 7.5 kg/m2 to 7.75 kg/m2 or 7.75 kg/m2 to 8 kg/m2) may produce different changes in prevalence. Changes in cut-off points for gait speed and grip strength had a limited impact on sarcopenia prevalence and on study comparability.

Accretion-induced luminosity spreads in young clusters: evidence from stellar rotation

NASA Astrophysics Data System (ADS)

Littlefair, S. P.; Naylor, Tim; Mayne, N. J.; Saunders, Eric; Jeffries, R. D.

2011-05-01

We present an analysis of the rotation of young stars in the associations Cepheus OB3b, NGC 2264, 2362 and the Orion Nebula Cluster (ONC). We discover a correlation between rotation rate and position in a colour-magnitude diagram (CMD) such that stars which lie above an empirically determined median pre-main sequence rotate more rapidly than stars which lie below this sequence. The same correlation is seen, with a high degree of statistical significance, in each association studied here. If position within the CMD is interpreted as being due to genuine age spreads within a cluster, then the stars above the median pre-main sequence would be the youngest stars. This would in turn imply that the most rapidly rotating stars in an association are the youngest, and hence those with the largest moments of inertia and highest likelihood of ongoing accretion. Such a result does not fit naturally into the existing picture of angular momentum evolution in young stars, where the stars are braked effectively by their accretion discs until the disc disperses. Instead, we argue that, for a given association of young stars, position within the CMD is not primarily a function of age, but of accretion history. We show that this hypothesis could explain the correlation we observe between rotation rate and position within the CMD.

[Occupational low back pain in nursing workers: massage versus pain].

PubMed

Borges, Talita Pavarini; Kurebayashi, Leonice Fumiko Sato; Silva, Maria Júlia Paes da

2014-08-01

To assess the efficacy of massage for decreasing occupational low back pain in workers of a Nursing team in an Emergency Room. A randomized controlled trial, using a socio-demographic/morbidity questionnaire and a Pain Numeric Scale. Forty-five subjects were randomly allocated for intervention (G1 - Massage by acupressure), placebo group (G2 - application of Garlium Arseneid Laser 904 nm turned off) and control (G3 - no intervention). The main triggering factor, as well as the worsening of pain, was the patient manipulation, both with a prevalence of 34.9%. The main treatment for low back pain before this research was the use of medication, with a prevalence of 44.2%. In accordance with a variance analysis, only G1 presented a significant statistical difference, with a better result after 12 sessions. Massage presented an enormous effect (d = 4.59), corresponding to 86% of reduction in the pain level. Massage was effective to decrease occupational low back pain of those Nursing workers.

Bend-insensitive single-mode photonic crystal fiber with ultralarge effective area for dual applications

NASA Astrophysics Data System (ADS)

Islam, Md. Asiful; Alam, M. Shah

2013-05-01

A novel photonic crystal fiber (PCF) having circular arrangement of cladding air holes has been designed and numerically optimized to obtain a bend insensitive single mode fiber with large mode area for both wavelength division multiplexing (WDM) communication and fiber-to-the-home (FTTH) application. The bending loss of the proposed bent PCF lies in the range of 10-3 to 10-4 dB/turn or lower over 1300 to 1700 nm, and 2 × 10-4 dB/turn at the wavelength of 1550 nm for a 30-mm bend radius with a higher order mode (HOM) cut-off frequency below 1200 nm for WDM application. When the whole structure of the PCF is scaled down, a bending loss of 6.78×10-4 dB/turn at 1550 nm for a 4-mm bend radius is obtained, and the loss remains in the order of 10-4 dB/turn over the same range of wavelength with an HOM cut-off frequency below 700 nm, and makes the fiber useful for FTTH applications. Furthermore, this structure is also optimized to show a splice loss near zero for fusion-splicing to a conventional single-mode fiber (SMF).

Cytidine-stabilized gold nanocluster as a fluorescence turn-on and turn-off probe for dual functional detection of Ag(+) and Hg(2+).

PubMed

Zhang, Yuanyuan; Jiang, Hui; Wang, Xuemei

2015-04-22

In this study, we have developed a label-free, dual functional detection strategy for highly selective and sensitive determination of aqueous Ag(+) and Hg(2+) by using cytidine stabilized Au NCs and AuAg NCs as fluorescent turn-on and turn off probes, respectively. The Au NCs and AuAg NCs showed a remarkably rapid response and high selectivity for Ag(+) and Hg(2+) over other metal ions, and relevant detection limit of Ag(+) and Hg(2+) is ca. 10 nM and 30 nM, respectively. Importantly, the fluorescence enhanced Au NCs by doping Ag(+) can be conveniently reusable for the detection of Hg(2+) based on the corresponding fluorescence quenching. The sensing mechanism was based on the high-affinity metallophilic Hg(2+)-Ag(+) interaction, which effectively quenched the fluorescence of AuAg NCs. Furthermore, these fluorescent nanoprobes could be readily applied to Ag(+) and Hg(2+) detection in environmental water samples, indicating their possibility to be utilized as a convenient, dual functional, rapid response, and label-free fluorescence sensor for related environmental and health monitoring. Copyright © 2015 Elsevier B.V. All rights reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hachisu, Izumi; Kato, Mariko, E-mail: hachisu@ea.c.u-tokyo.ac.jp, E-mail: mariko@educ.cc.keio.ac.jp

We identified a general course of classical nova outbursts in the B – V versus U – B color-color diagram. It is reported that novae show spectra similar to those of A-F supergiants near optical light maximum. However, they do not follow the supergiant sequence in the color-color diagram, neither the blackbody nor the main-sequence sequence. Instead, we found that novae evolve along a new sequence in the pre-maximum and near-maximum phases, which we call 'the nova-giant sequence'. This sequence is parallel to but Δ(U – B) ≈ –0.2 mag bluer than the supergiant sequence. This is because the massmore » of a nova envelope is much (∼10{sup –4} times) less than that of a normal supergiant. After optical maximum, its color quickly evolves back blueward along the same nova-giant sequence and reaches the point of free-free emission (B – V = –0.03, U – B = –0.97), which coincides with the intersection of the blackbody sequence and the nova-giant sequence, and remains there for a while. Then the color evolves leftward (blueward in B – V but almost constant in U – B), owing mainly to the development of strong emission lines. This is the general course of nova outbursts in the color-color diagram, which was deduced from eight well-observed novae in various speed classes. For a nova with unknown extinction, we can determine a reliable value of the color excess by matching the observed track of the target nova with this general course. This is a new and convenient method for obtaining the color excesses of classical novae. Using this method, we redetermined the color excesses of 20 well-observed novae. The obtained color excesses are in reasonable agreement with the previous results, which in turn support the idea of our general track of nova outbursts. Additionally, we estimated the absolute V magnitudes of about 30 novae using a method for time-stretching nova light curves to analyze the distance-reddening relations of the novae.« less

Kinematics of center of mass and center of pressure predict friction requirement at shoe-floor interface during walking.

PubMed

Yamaguchi, Takeshi; Yano, Masaru; Onodera, Hiroshi; Hokkirigawa, Kazuo

2013-06-01

We aimed to determine whether inclination angles of the line connecting the whole body center of mass (COM) to the center of pressure (COP) (COM-COP angle) help predict the required coefficient of friction (RCOF) in young adult males during the weight acceptance and push-off phases in transient movements such as turning, gait termination and initiation, and steady-state movements such as straight walking. Seventeen healthy young adult males were asked to (1) walk in a straight line, (2) turn 60° with either foot (step and spin turns), and (3) initiate and terminate walking on a dry level floor. Peak absolute values of the ratio between resultant horizontal and vertical ground reaction forces during the weight acceptance and push-off phases (RCOFh and RCOFt, respectively) were calculated. COM-COP angles θh and θt at the instant of RCOFh and RCOFt, respectively, were also calculated. Bivariate regression analysis demonstrated that the |θh| and |θt| tangents were significant predictors of RCOFh (R = 0.878; R(2) = 0.770; p<0.001) and RCOFt (R = 0.918; R(2) = 0.843; p<0.001), respectively. The results suggest that COM and COP kinematics (i.e., the COM-COP angle) serve as a predictor of friction requirement during the weight acceptance and push-off phases in steady-state movements such as straight walking and transient movements such as turning as well as gait termination and initiation. Copyright © 2012 Elsevier B.V. All rights reserved.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Boyack, B.E.; Steiner, J.L.; Harmony, S.C.

The PIUS Advanced Reactor is a 640-MW(e) pressurized-water reactor developed by Asea Brown Boveri. A unique feature of the PIUS concept is the absence of mechanical control and shutdown rods. Reactivity normally is controlled by the boron concentration in the coolant and the temperature of the moderator coolant. Analyses of five initiating events have been completed on the basis of calculations performed with the system neutronic and thermal-hydraulic analysis code TRAC-PF1/MOD2. The initiating events analyzed are (1) reactor scram, (2) loss of off-site power (3) main steam-line break, (4) small-break loss of coolant, and (5) large-break loss of coolant. Inmore » addition to the baseline calculation for each sequence, sensitivity studies were performed to explore the response of the PIUS reactor to severe off-normal conditions having a very low probability of occurrence. The sensitivity studies provide insights into the robustness of the design.« less

Conformational dynamics of a short antigenic peptide in its free and antibody bound forms gives insight into the role of β-turns in peptide immunogenicity.

PubMed

Shukla, Rashmi Tambe; Sasidhar, Yellamraju U

2015-07-01

Earlier immunological experiments with a synthetic 36-residue peptide (75-110) from Influenza hemagglutinin have been shown to elicit anti-peptide antibodies (Ab) which could cross-react with the parent protein. In this article, we have studied the conformational features of a short antigenic (Ag) peptide ((98)YPYDVPDYASLRS(110)) from Influenza hemagglutinin in its free and antibody (Ab) bound forms with molecular dynamics simulations using GROMACS package and OPLS-AA/L all-atom force field at two different temperatures (293 K and 310 K). Multiple simulations for the free Ag peptide show sampling of ordered conformations and suggest different conformational preferences of the peptide at the two temperatures. The free Ag samples a conformation crucial for Ab binding (β-turn formed by "DYAS" sequence) with greater preference at 310 K while, it samples a native-like conformation with relatively greater propensity at 293 K. The sequence "DYAS" samples β-turn conformation with greater propensity at 310 K as part of the hemagglutinin protein also. The bound Ag too samples the β-turn involving "DYAS" sequence and in addition it also samples a β-turn formed by the sequence "YPYD" at its N-terminus, which seems to be induced upon binding to the Ab. Further, the bound Ag displays conformational flexibility at both 293 K and 310 K, particularly at terminal residues. The implications of these results for peptide immunogenicity and Ag-Ab recognition are discussed. © 2015 Wiley Periodicals, Inc.

Higher-order Skyrme hair of black holes

NASA Astrophysics Data System (ADS)

Gudnason, Sven Bjarke; Nitta, Muneto

2018-05-01

Higher-order derivative terms are considered as replacement for the Skyrme term in an Einstein-Skyrme-like model in order to pinpoint which properties are necessary for a black hole to possess stable static scalar hair. We find two new models able to support stable black hole hair in the limit of the Skyrme term being turned off. They contain 8 and 12 derivatives, respectively, and are roughly the Skyrme-term squared and the so-called BPS-Skyrme-term squared. In the twelfth-order model we find that the lower branches, which are normally unstable, become stable in the limit where the Skyrme term is turned off. We check this claim with a linear stability analysis. Finally, we find for a certain range of the gravitational coupling and horizon radius, that the twelfth-order model contains 4 solutions as opposed to 2. More surprisingly, the lowest part of the would-be unstable branch turns out to be the stable one of the 4 solutions.

Advanced insulated gate bipolar transistor gate drive

DOEpatents

Short, James Evans [Monongahela, PA; West, Shawn Michael [West Mifflin, PA; Fabean, Robert J [Donora, PA

2009-08-04

A gate drive for an insulated gate bipolar transistor (IGBT) includes a control and protection module coupled to a collector terminal of the IGBT, an optical communications module coupled to the control and protection module, a power supply module coupled to the control and protection module and an output power stage module with inputs coupled to the power supply module and the control and protection module, and outputs coupled to a gate terminal and an emitter terminal of the IGBT. The optical communications module is configured to send control signals to the control and protection module. The power supply module is configured to distribute inputted power to the control and protection module. The control and protection module outputs on/off, soft turn-off and/or soft turn-on signals to the output power stage module, which, in turn, supplies a current based on the signal(s) from the control and protection module for charging or discharging an input capacitance of the IGBT.

Homogeneous double-layer amorphous Si-doped indium oxide thin-film transistors for control of turn-on voltage

DOE Office of Scientific and Technical Information (OSTI.GOV)

Kizu, Takio, E-mail: KIZU.Takio@nims.go.jp, E-mail: TSUKAGOSHI.Kazuhito@nims.go.jp; Tsukagoshi, Kazuhito, E-mail: KIZU.Takio@nims.go.jp, E-mail: TSUKAGOSHI.Kazuhito@nims.go.jp; Aikawa, Shinya

We fabricated homogeneous double-layer amorphous Si-doped indium oxide (ISO) thin-film transistors (TFTs) with an insulating ISO cap layer on top of a semiconducting ISO bottom channel layer. The homogeneously stacked ISO TFT exhibited high mobility (19.6 cm{sup 2}/V s) and normally-off characteristics after annealing in air. It exhibited normally-off characteristics because the ISO insulator suppressed oxygen desorption, which suppressed the formation of oxygen vacancies (V{sub O}) in the semiconducting ISO. Furthermore, we investigated the recovery of the double-layer ISO TFT, after a large negative shift in turn-on voltage caused by hydrogen annealing, by treating it with annealing in ozone. The recoverymore » in turn-on voltage indicates that the dense V{sub O} in the semiconducting ISO can be partially filled through the insulator ISO. Controlling molecule penetration in the homogeneous double layer is useful for adjusting the properties of TFTs in advanced oxide electronics.« less

Fast gray-to-gray switching of a hybrid-aligned liquid crystal cell

NASA Astrophysics Data System (ADS)

Choi, Tae-Hoon; Kim, Jung-Wook; Yoon, Tae-Hoon

2015-03-01

We demonstrate fast gray-to-gray (GTG) switching of a hybrid-aligned liquid crystal cell by applying both vertical and inplane electric fields to liquid crystals (LCs) using a four-terminal electrode structure. The LCs are switched to the bright state through downward tilting and twist deformation initiated by applying an in-plane electric field, whereas they are switched back to the initial dark state through optically hidden relaxation initiated by applying a vertical electric field for a short duration. The top electrode in the proposed device is grounded, which requires a much higher voltage to be applied for in-plane rotation of LCs. Thus, ultrafast turn-on switching of the device is achieved, whereas the turn-off switching of the proposed device is independent of the elastic constants and the viscosity of the LCs so that fast turn-off switching can be achieved. We experimentally obtained a total response time of 0.75 ms. Furthermore, fast GTG response within 3 ms could be achieved.

Prediction of β-turns in proteins from multiple alignment using neural network

PubMed Central

Kaur, Harpreet; Raghava, Gajendra Pal Singh

2003-01-01

A neural network-based method has been developed for the prediction of β-turns in proteins by using multiple sequence alignment. Two feed-forward back-propagation networks with a single hidden layer are used where the first-sequence structure network is trained with the multiple sequence alignment in the form of PSI-BLAST–generated position-specific scoring matrices. The initial predictions from the first network and PSIPRED-predicted secondary structure are used as input to the second structure-structure network to refine the predictions obtained from the first net. A significant improvement in prediction accuracy has been achieved by using evolutionary information contained in the multiple sequence alignment. The final network yields an overall prediction accuracy of 75.5% when tested by sevenfold cross-validation on a set of 426 nonhomologous protein chains. The corresponding Qpred, Qobs, and Matthews correlation coefficient values are 49.8%, 72.3%, and 0.43, respectively, and are the best among all the previously published β-turn prediction methods. The Web server BetaTPred2 (http://www.imtech.res.in/raghava/betatpred2/) has been developed based on this approach. PMID:12592033

Pathological tremor prediction using surface EMG and acceleration: potential use in “ON-OFF” demand driven deep brain stimulator design

PubMed Central

Basu, Ishita; Graupe, Daniel; Tuninetti, Daniela; Shukla, Pitamber; Slavin, Konstantin V.; Metman, Leo Verhagen; Corcos, Daniel M.

2013-01-01

Objective We present a proof of concept for a novel method of predicting the onset of pathological tremor using non-invasively measured surface electromyogram (sEMG) and acceleration from tremor-affected extremities of patients with Parkinson’s disease (PD) and Essential tremor (ET). Approach The tremor prediction algorithm uses a set of spectral (fourier and wavelet) and non-linear time series (entropy and recurrence rate) parameters extracted from the non-invasively recorded sEMG and acceleration signals. Main results The resulting algorithm is shown to successfully predict tremor onset for all 91 trials recorded in 4 PD patients and for all 91 trials recorded in 4 ET patients. The predictor achieves a 100% sensitivity for all trials considered, along with an overall accuracy of 85.7% for all ET trials and 80.2% for all PD trials. By using a Pearson’s chi-square test, the prediction results are shown to significantly differ from a random prediction outcome. Significance The tremor prediction algorithm can be potentially used for designing the next generation of non-invasive closed-loop predictive ON-OFF controllers for deep brain stimulation (DBS), used for suppressing pathological tremor in such patients. Such a system is based on alternating ON and OFF DBS periods, an incoming tremor being predicted during the time intervals when DBS is OFF, so as to turn DBS back ON. The prediction should be a few seconds before tremor re-appears so that the patient is tremor-free for the entire DBS ON-OFF cycle as well as the tremor-free DBS OFF interval should be maximized in order to minimize the current injected in the brain and battery usage. PMID:23658233

Pathological tremor prediction using surface electromyogram and acceleration: potential use in ‘ON-OFF’ demand driven deep brain stimulator design

NASA Astrophysics Data System (ADS)

Basu, Ishita; Graupe, Daniel; Tuninetti, Daniela; Shukla, Pitamber; Slavin, Konstantin V.; Verhagen Metman, Leo; Corcos, Daniel M.

2013-06-01

Objective. We present a proof of concept for a novel method of predicting the onset of pathological tremor using non-invasively measured surface electromyogram (sEMG) and acceleration from tremor-affected extremities of patients with Parkinson’s disease (PD) and essential tremor (ET). Approach. The tremor prediction algorithm uses a set of spectral (Fourier and wavelet) and nonlinear time series (entropy and recurrence rate) parameters extracted from the non-invasively recorded sEMG and acceleration signals. Main results. The resulting algorithm is shown to successfully predict tremor onset for all 91 trials recorded in 4 PD patients and for all 91 trials recorded in 4 ET patients. The predictor achieves a 100% sensitivity for all trials considered, along with an overall accuracy of 85.7% for all ET trials and 80.2% for all PD trials. By using a Pearson’s chi-square test, the prediction results are shown to significantly differ from a random prediction outcome. Significance. The tremor prediction algorithm can be potentially used for designing the next generation of non-invasive closed-loop predictive ON-OFF controllers for deep brain stimulation (DBS), used for suppressing pathological tremor in such patients. Such a system is based on alternating ON and OFF DBS periods, an incoming tremor being predicted during the time intervals when DBS is OFF, so as to turn DBS back ON. The prediction should be a few seconds before tremor re-appears so that the patient is tremor-free for the entire DBS ON-OFF cycle and the tremor-free DBS OFF interval should be maximized in order to minimize the current injected in the brain and battery usage.

A Multicontrolled Enamine Configurational Switch Undergoing Dynamic Constitutional Exchange.

PubMed

Ren, Yansong; Svensson, Per H; Ramström, Olof

2018-05-22

A multiresponsive enamine-based molecular switch is presented, in which forward/backward configurational rotation around the C=C bond could be precisely controlled by the addition of an acid/base or metal ions. Fluorescence turn-on/off effects and large Stokes shifts were observed while regulating the switching process with Cu II . The enamine functionality furthermore enabled double dynamic regimes, in which configurational switching could operate in conjunction with constitutional enamine exchange of the rotor part. This behavior was used to construct a prototypical dynamic covalent switch system through enamine exchange with primary amines. The dynamic exchange process could be readily turned on/off by regulating the switch status with pH. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

A facile fluorescent "turn-off" method for sensing paraquat based on pyranine-paraquat interaction

NASA Astrophysics Data System (ADS)

Zhao, Zuzhi; Zhang, Fengwei; Zhang, Zipin

2018-06-01

Development of a technically simple yet effective method for paraquat (PQ) detection is of great importance due to its high clinical and environmental relevance. In this study, we developed a pyranine-based fluorescent "turn-off" method for PQ sensing based on pyranine-PQ interaction. We investigated the dependence of analytical performance of this method on the experimental conditions, such as the ion strength, medium pH, and so on. Under the optimized conditions, the method is sensitive and selective, and could be used for PQ detection in real-world sample. This study essentially provides a readily accessible fluorescent system for PQ sensing which is cheap, robust, and technically simple, and it is envisaged to find more interesting clinical and environmental applications.

Control Circuit For Two Stepping Motors

NASA Technical Reports Server (NTRS)

Ratliff, Roger; Rehmann, Kenneth; Backus, Charles

1990-01-01

Control circuit operates two independent stepping motors, one at a time. Provides following operating features: After selected motor stepped to chosen position, power turned off to reduce dissipation; Includes two up/down counters that remember at which one of eight steps each motor set. For selected motor, step indicated by illumination of one of eight light-emitting diodes (LED's) in ring; Selected motor advanced one step at time or repeatedly at rate controlled; Motor current - 30 mA at 90 degree positions, 60 mA at 45 degree positions - indicated by high or low intensity of LED that serves as motor-current monitor; Power-on reset feature provides trouble-free starts; To maintain synchronism between control circuit and motors, stepping of counters inhibited when motor power turned off.

Electrical servo actuator bracket. [fuel control valves on jet engines

NASA Technical Reports Server (NTRS)

Sawyer, R. V. (Inventor)

1981-01-01

An electrical servo actuator is mounted on a support arm which is allowed to pivot on a bolt through a fixed mounting bracket. The actuator is pivotally connected to the end of the support arm by a bolt which has an extension allowed to pass through a slot in the fixed mounting bracket. An actuator rod extends from the servo actuator to a crank arm which turns a control shaft. A short linear thrust of the rod pivots the crank arm through about 90 for full-on control with the rod contracted into the servo actuator, and full-off control when the rod is extended from the actuator. A spring moves the servo actuator and actuator rod toward the control crank arm once the actuator rod is fully extended in the full-off position. This assures the turning of the control shaft to a full-off position. A stop bolt and slot are provided to limit pivot motion. Once fully extended, the spring pivots the motion.

Nuclear export receptor CRM1 recognizes diverse conformations in nuclear export signals.

PubMed

Fung, Ho Yee Joyce; Fu, Szu-Chin; Chook, Yuh Min

2017-03-10

Nuclear export receptor CRM1 binds highly variable nuclear export signals (NESs) in hundreds of different cargoes. Previously we have shown that CRM1 binds NESs in both polypeptide orientations (Fung et al., 2015). Here, we show crystal structures of CRM1 bound to eight additional NESs which reveal diverse conformations that range from loop-like to all-helix, which occupy different extents of the invariant NES-binding groove. Analysis of all NES structures show 5-6 distinct backbone conformations where the only conserved secondary structural element is one turn of helix that binds the central portion of the CRM1 groove. All NESs also participate in main chain hydrogen bonding with human CRM1 Lys568 side chain, which acts as a specificity filter that prevents binding of non-NES peptides. The large conformational range of NES backbones explains the lack of a fixed pattern for its 3-5 hydrophobic anchor residues, which in turn explains the large array of peptide sequences that can function as NESs.

Fungicide Sensitivity and Characterization of Cobweb Disease on a Pleurotus eryngii Mushroom Crop Caused by Cladobotryum mycophilum

PubMed Central

Kim, Min Keun; Seuk, Su Won; Lee, Young Han; Kim, Hye Ran; Cho, Kye Man

2014-01-01

In 2009–2010, unusual symptoms were observed on Pleurotus eryngii grown in mushroom farms in Gyeongnam Province, Republic of Korea. One of the main symptoms was a cobweb-like growth of fungal mycelia over the surface of the mushroom. The colonies on the surface rapidly overwhelmed the mushrooms and developed several spores within 3–4 days. The colonized surface turned pale brown or yellow. The fruit body eventually turned dark brown and became rancid. Koch’s postulates were completed by spraying and spotting using isolated strains. The phylogenetic tree obtained from the internal transcribed spacer sequence analysis showed that the isolated fungal pathogen corresponded to Cladobotryum mycophilum (99.5%). In the fungicide sensitivity tests, the ED50 values for the isolate with respect to benomyl and carbendazim were from 0.29 to 0.31 ppm. Benzimidazole fungicides were most effective against C. mycophilum, a causal agent of cobweb disease in P. eryngii. PMID:25288989

[A turning point in the knowledge of the structure-function-activity relations of elastin].

PubMed

Alix, A J

2001-01-01

In this review are presented the last new results of our research group dealing with the molecular structures (atomic level) of tropoelastin, elastin and elastin derived peptides studied by using essentially methods of bioinformatics (theoretical predictions and molecular modelling) linked to experimental circular dichroism spectroscopic studies. We already had characterized both the local secondary structure and some parts of the tertiary structure of the tropoelastin and elastin molecules (human, bovine...), by using either theoretical predictions (local secondary structure, linear epitopes...) and/or experimental data (optical spectroscopic methods: Raman scattering, infrared absorption, circular dichroism). Except the cross-linking regions which are in helical conformations, the whole tropoelastin structure displays a lot of beta-reverse turns which usually belong to irregular structures in proteins. These turns play a key role in other regularly structures orientation (alpha-helix, beta-strand), thus they are very important in the native protein 3D architecture. It is particularly true for human tropoelastin, because its sequence is rich in glycines and prolines, and these residues are frequently met in beta-turns (a beta-turn is made of four consecutive residues which are stabilized by an hydrogen bond). Several types of beta-turns can be defined with the dihedral angles values phi and psi of the two central residues. Thus, by using a very recent updated set of propensities for the amino acid residues to belong to given types of reverse beta-turns (extracted from a reference set of known 3-D structures of globular proteins), we have determined, (by using our home made software COUDES), for all possible tetrapeptides of the human tropoelastin sequence, the distribution and the characterization of the possible type of turns. Thus, it is shown that the locations and/or the types of these reverse beta-turns reveal a regularity and are not all random. This confirms our hypothesis that intra-molecular elasticity of tropoelastin could be explained by the possibility of transitions between conformations involving short beta-strands and beta-turns. This result is of great interest in the construction (by using molecular biology) of elastic biomaterials derived from the elastin sequence (particularly, the elastin derived peptides corresponding to the sequence exon 21--(exon 24--exon 24...). Our study permit also to predict the conformations of specific elastin derived peptides which could have interesting biological activity. Peptides resulting from the degradation of elastin, the insoluble polymer of tropoelastin and responsible for the elasticity of vertebrate tissues, can induce biological effects and notably the regulation of matrix metalloproteinases (MMP-s) activity. Recently, it was proposed that some elastin derived hexapeptides resulting from circular permutations of VGVAPG (a three fold repetition sequence in exon 24 of human tropoelastin) possess MMP-1 production and activation regulation properties. This effect depends on the presence of the tropoelastin specific membraneous receptor 67 KDa EBP (Elastin Binding Protein). Our results obtained by using both circular dichroism spectroscopy and linear predictions confirmed the hypothesis of a structure dependent mechanism with a possibly occurring type VIII beta-turn on the first four residues of the GXXPG sequence consensus which is only present among all active peptides. Thus, we have performed extensive molecular dynamics studies, in both implicit and explicit solvent, on these active and inactive elastin derived hexapeptides. Using our own analysis method of pattern recognition of the types of the beta-reverse-turns followed during the molecular dynamics trajectory, we found that active and inactive peptides effectively form two well distinct conformational groups in which active peptides preferentially adopt conformation close to type VIII GXXP (beta-reverse-turn. The structural role of the C terminal G residue could also be explained. Additional molecular simulations on (VGVAPG)2 and (VGVAPG)3 show the formation of two or three GXXP tetrapeptides adopting a structure close to type VIII beta-reverse-turn, suggesting a local conformational preference for this motif. This observation of a specific structural single and/or repeated motif is in agreement with the circular dichroism spectra of the involved (VGVAPG)1, (VGVAPG)2 and (VGVAPG)3 peptides and then it can be proposed that their biological activities have to be linear. The final aim of this type of work is to understand more about the sequence/structure/function/activity relationships of those structured peptides in order to propose specific sequences (corresponding to specific structures) for best biological activity results.

Whole-Genome Thermodynamic Analysis Reduces siRNA Off-Target Effects

PubMed Central

Chen, Xi; Liu, Peng; Chou, Hui-Hsien

2013-01-01

Small interfering RNAs (siRNAs) are important tools for knocking down targeted genes, and have been widely applied to biological and biomedical research. To design siRNAs, two important aspects must be considered: the potency in knocking down target genes and the off-target effect on any nontarget genes. Although many studies have produced useful tools to design potent siRNAs, off-target prevention has mostly been delegated to sequence-level alignment tools such as BLAST. We hypothesize that whole-genome thermodynamic analysis can identify potential off-targets with higher precision and help us avoid siRNAs that may have strong off-target effects. To validate this hypothesis, two siRNA sets were designed to target three human genes IDH1, ITPR2 and TRIM28. They were selected from the output of two popular siRNA design tools, siDirect and siDesign. Both siRNA design tools have incorporated sequence-level screening to avoid off-targets, thus their output is believed to be optimal. However, one of the sets we tested has off-target genes predicted by Picky, a whole-genome thermodynamic analysis tool. Picky can identify off-target genes that may hybridize to a siRNA within a user-specified melting temperature range. Our experiments validated that some off-target genes predicted by Picky can indeed be inhibited by siRNAs. Similar experiments were performed using commercially available siRNAs and a few off-target genes were also found to be inhibited as predicted by Picky. In summary, we demonstrate that whole-genome thermodynamic analysis can identify off-target genes that are missed in sequence-level screening. Because Picky prediction is deterministic according to thermodynamics, if a siRNA candidate has no Picky predicted off-targets, it is unlikely to cause off-target effects. Therefore, we recommend including Picky as an additional screening step in siRNA design. PMID:23484018

Applications of next-generation sequencing analysis for the detection of hepatocellular carcinoma-associated hepatitis B virus mutations.

PubMed

Wu, I-Chin; Liu, Wen-Chun; Chang, Ting-Tsung

2018-06-02

Next-generation sequencing (NGS) is a powerful and high-throughput method for the detection of viral mutations. This article provides a brief overview about optimization of NGS analysis for hepatocellular carcinoma (HCC)-associated hepatitis B virus (HBV) mutations, and hepatocarcinogenesis of relevant mutations. For the application of NGS analysis in the genome of HBV, four noteworthy steps were discovered in testing. First, a sample-specific reference sequence was the most effective mapping reference for NGS. Second, elongating the end of reference sequence improved mapping performance at the end of the genome. Third, resetting the origin of mapping reference sequence could probed deletion mutations and variants at a certain location with common mutations. Fourth, using a platform-specific cut-off value to distinguish authentic minority variants from technical artifacts was found to be highly effective. One hundred and sixty-seven HBV single nucleotide variants (SNVs) were found to be studied previously through a systematic literature review, and 12 SNVs were determined to be associated with HCC by meta-analysis. From comprehensive research using a HBV genome-wide NGS analysis, 60 NGS-defined HCC-associated SNVs with their pathogenic frequencies were identified, with 19 reported previously. All the 12 HCC-associated SNVs proved by meta-analysis were confirmed by NGS analysis, except for C1766T and T1768A which were mainly expressed in genotypes A and D, but including the subgroup analysis of A1762T. In the 41 novel NGS-defined HCC-associated SNVs, 31.7% (13/41) had cut-off values of SNV frequency lower than 20%. This showed that NGS could be used to detect HCC-associated SNVs with low SNV frequency. Most SNV II (the minor strains in the majority of non-HCC patients) had either low (< 20%) or high (> 80%) SNV frequencies in HCC patients, a characteristic U-shaped distribution pattern. The cut-off values of SNV frequency for HCC-associated SNVs represent their pathogenic frequencies. The pathogenic frequencies of HCC-associated SNV II also showed a U-shaped distribution. Hepatocarcinogenesis induced by HBV mutated proteins through cellular pathways was reviewed. NGS analysis is useful to discover novel HCC-associated HBV SNVs, especially those with low SNV frequency. The hepatocarcinogenetic mechanisms of novel HCC-associated HBV SNVs defined by NGS analysis deserve further investigation.

Powering Down from the Bottom up: Greener Client Computing

ERIC Educational Resources Information Center

O'Donnell, Tom

2009-01-01

A decade ago, people wanting to practice "green computing" recycled their printer paper, turned their personal desktop systems off from time to time, and tried their best to donate old equipment to a nonprofit instead of throwing it away. A campus IT department can shave a few watts off just about any IT process--the real trick is planning and…

The 2012 Ferrara seismic sequence: Regional crustal structure, earthquake sources, and seismic hazard

NASA Astrophysics Data System (ADS)

Malagnini, Luca; Herrmann, Robert B.; Munafò, Irene; Buttinelli, Mauro; Anselmi, Mario; Akinci, Aybige; Boschi, E.

2012-10-01

Inadequate seismic design codes can be dangerous, particularly when they underestimate the true hazard. In this study we use data from a sequence of moderate-sized earthquakes in northeast Italy to validate and test a regional wave propagation model which, in turn, is used to understand some weaknesses of the current design spectra. Our velocity model, while regionalized and somewhat ad hoc, is consistent with geophysical observations and the local geology. In the 0.02-0.1 Hz band, this model is validated by using it to calculate moment tensor solutions of 20 earthquakes (5.6 ≥ MW ≥ 3.2) in the 2012 Ferrara, Italy, seismic sequence. The seismic spectra observed for the relatively small main shock significantly exceeded the design spectra to be used in the area for critical structures. Observations and synthetics reveal that the ground motions are dominated by long-duration surface waves, which, apparently, the design codes do not adequately anticipate. In light of our results, the present seismic hazard assessment in the entire Pianura Padana, including the city of Milan, needs to be re-evaluated.

Alternative Splicing as a Target for Cancer Treatment.

PubMed

Martinez-Montiel, Nancy; Rosas-Murrieta, Nora Hilda; Anaya Ruiz, Maricruz; Monjaraz-Guzman, Eduardo; Martinez-Contreras, Rebeca

2018-02-11

Alternative splicing is a key mechanism determinant for gene expression in metazoan. During alternative splicing, non-coding sequences are removed to generate different mature messenger RNAs due to a combination of sequence elements and cellular factors that contribute to splicing regulation. A different combination of splicing sites, exonic or intronic sequences, mutually exclusive exons or retained introns could be selected during alternative splicing to generate different mature mRNAs that could in turn produce distinct protein products. Alternative splicing is the main source of protein diversity responsible for 90% of human gene expression, and it has recently become a hallmark for cancer with a full potential as a prognostic and therapeutic tool. Currently, more than 15,000 alternative splicing events have been associated to different aspects of cancer biology, including cell proliferation and invasion, apoptosis resistance and susceptibility to different chemotherapeutic drugs. Here, we present well established and newly discovered splicing events that occur in different cancer-related genes, their modification by several approaches and the current status of key tools developed to target alternative splicing with diagnostic and therapeutic purposes.

Stable scalable control of soliton propagation in broadband nonlinear optical waveguides

NASA Astrophysics Data System (ADS)

Peleg, Avner; Nguyen, Quan M.; Huynh, Toan T.

2017-02-01

We develop a method for achieving scalable transmission stabilization and switching of N colliding soliton sequences in optical waveguides with broadband delayed Raman response and narrowband nonlinear gain-loss. We show that dynamics of soliton amplitudes in N-sequence transmission is described by a generalized N-dimensional predator-prey model. Stability and bifurcation analysis for the predator-prey model are used to obtain simple conditions on the physical parameters for robust transmission stabilization as well as on-off and off-on switching of M out of N soliton sequences. Numerical simulations for single-waveguide transmission with a system of N coupled nonlinear Schrödinger equations with 2 ≤ N ≤ 4 show excellent agreement with the predator-prey model's predictions and stable propagation over significantly larger distances compared with other broadband nonlinear single-waveguide systems. Moreover, stable on-off and off-on switching of multiple soliton sequences and stable multiple transmission switching events are demonstrated by the simulations. We discuss the reasons for the robustness and scalability of transmission stabilization and switching in waveguides with broadband delayed Raman response and narrowband nonlinear gain-loss, and explain their advantages compared with other broadband nonlinear waveguides.

New cataclysmic variables and other exotic binaries in the globular cluster 47 Tucanae*

NASA Astrophysics Data System (ADS)

Rivera Sandoval, L. E.; van den Berg, M.; Heinke, C. O.; Cohn, H. N.; Lugger, P. M.; Anderson, J.; Cool, A. M.; Edmonds, P. D.; Wijnands, R.; Ivanova, N.; Grindlay, J. E.

2018-04-01

We present 22 new (+3 confirmed) cataclysmic variables (CVs) in the non-core-collapsed globular cluster 47 Tucanae (47 Tuc). The total number of CVs in the cluster is now 43, the largest sample in any globular cluster so far. For the identifications we used near-ultraviolet (NUV) and optical images from the Hubble Space Telescope, in combination with X-ray results from the Chandra X-ray Observatory. This allowed us to build the deepest NUV CV luminosity function of the cluster to date. We found that the CVs in 47 Tuc are more concentrated towards the cluster centre than the main-sequence turn-off stars. We compared our results to the CV populations of the core-collapsed globular clusters NGC 6397 and NGC 6752. We found that 47 Tuc has fewer bright CVs per unit mass than those two other clusters. That suggests that dynamical interactions in core-collapsed clusters play a major role creating new CVs. In 47 Tuc, the CV population is probably dominated by primordial and old dynamically formed systems. We estimated that the CVs in 47 Tuc have total masses of ˜1.4 M⊙. We also found that the X-ray luminosity function of the CVs in the three clusters is bimodal. Additionally, we discuss a possible double degenerate system and an intriguing/unclassified object. Finally, we present four systems that could be millisecond pulsar companions given their X-ray and NUV/optical colours. For one of them we present very strong evidence for being an ablated companion. The other three could be CO or He white dwarfs.

CROSS-DISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY: Characteristics of alternating current hopping conductivity in DNA sequences

NASA Astrophysics Data System (ADS)

Ma, Song-Shan; Xu, Hui; Wang, Huan-You; Guo, Rui

2009-08-01

This paper presents a model to describe alternating current (AC) conductivity of DNA sequences, in which DNA is considered as a one-dimensional (1D) disordered system, and electrons transport via hopping between localized states. It finds that AC conductivity in DNA sequences increases as the frequency of the external electric field rises, and it takes the form of øac(ω) ~ ω2 ln2(1/ω). Also AC conductivity of DNA sequences increases with the increase of temperature, this phenomenon presents characteristics of weak temperature-dependence. Meanwhile, the AC conductivity in an off-diagonally correlated case is much larger than that in the uncorrelated case of the Anderson limit in low temperatures, which indicates that the off-diagonal correlations in DNA sequences have a great effect on the AC conductivity, while at high temperature the off-diagonal correlations no longer play a vital role in electric transport. In addition, the proportion of nucleotide pairs p also plays an important role in AC electron transport of DNA sequences. For p < 0.5, the conductivity of DNA sequence decreases with the increase of p, while for p >= 0.5, the conductivity increases with the increase of p.

How To Write a Business Letter. Power of the Printed Word.

ERIC Educational Resources Information Center

Forbes, Malcolm

Business letters should turn people on rather than turning them off. To write a good business letter, know what the goal is before starting to write, call the reader by name, tell what the letter is about in the first paragraph, refer to dates when answering letters, and write from the reader's point of view. Be positive, be nice, and be natural.…

A bi-stable nanoelectromechanical non-volatile memory based on van der Waals force

NASA Astrophysics Data System (ADS)

Soon, Bo Woon; Jiaqiang Ng, Eldwin; Qian, You; Singh, Navab; Julius Tsai, Minglin; Lee, Chengkuo

2013-07-01

By using complementary-metal-oxide-semiconductor processes, a silicon based bi-stable nanoelectromechanical non-volatile memory is fabricated and characterized. The main feature of this device is an 80 nm wide and 3 μm high silicon nanofin (SiNF) of a high aspect ratio (1:35). The switching mechanism is realized by electrostatic actuation between two lateral electrodes, i.e., terminals. Bi-stable hysteresis behavior is demonstrated when the SiNF maintains its contact to one of the two terminals by leveraging on van der Waals force even after voltage bias is turned off. The compelling results indicate that this design is promising for realization of high density non-volatile memory application due to its nano-scale footprint and zero on-hold power consumption.

Two-photon LIF on the HIT-SI3 experiment: Absolute density and temperature measurements of deuterium neutrals

NASA Astrophysics Data System (ADS)

Elliott, Drew; Sutherland, Derek; Siddiqui, Umair; Scime, Earl; Everson, Chris; Morgan, Kyle; Hossack, Aaron; Nelson, Brian; Jarboe, Tom

2016-11-01

Two-photon laser-induced fluorescence measurements were performed on the helicity injected torus (HIT-SI3) device to determine the density and temperature of the background neutral deuterium population. Measurements were taken in 2 ms long pulsed plasmas after the inductive helicity injectors were turned off. Attempts to measure neutrals during the main phase of the plasma were unsuccessful, likely due to the density of neutrals being below the detection threshold of the diagnostic. An unexpectedly low density of atomic deuterium was measured in the afterglow; roughly 100 times lower than the theoretical prediction of 1017 m-3. The neutral temperatures measured were on the order of 1 eV. Temporally and spatially resolved neutral density and temperature data are presented.

Design and implementation of green intelligent lights based on the ZigBee

NASA Astrophysics Data System (ADS)

Gan, Yong; Jia, Chunli; Zou, Dongyao; Yang, Jiajia; Guo, Qianqian

2013-03-01

By analysis of the low degree of intelligence of the traditional lighting control methods, the paper uses the singlechip microcomputer for the control core, and uses a pyroelectric infrared technology to detect the existence of the human body, light sensors to sense the light intensity; the interface uses infrared sensor module, photosensitive sensor module, relay module to transmit the signal, which based on ZigBee wireless network. The main function of the design is to realize that the lighting can intelligently adjust the brightness according to the indoor light intensity when people in door, and it can turn off the light when people left. The circuit and program design of this system is flexible, and the system achieves the effect of intelligent energy saving control.

Analysis of beta-carotene hydroxylase gene cDNA isolated from the American oil-palm (Elaeis oleifera) mesocarp tissue cDNA library

PubMed Central

Bhore, Subhash J; Kassim, Amelia; Loh, Chye Ying; Shah, Farida H

2010-01-01

It is well known that the nutritional quality of the American oil-palm (Elaeis oleifera) mesocarp oil is superior to that of African oil-palm (Elaeis guineensis Jacq. Tenera) mesocarp oil. Therefore, it is of important to identify the genetic features for its superior value. This could be achieved through the genome sequencing of the oil-palm. However, the genome sequence is not available in the public domain due to commercial secrecy. Hence, we constructed a cDNA library and generated expressed sequence tags (3,205) from the mesocarp tissue of the American oil-palm. We continued to annotate each of these cDNAs after submitting to GenBank/DDBJ/EMBL. A rough analysis turned our attention to the beta-carotene hydroxylase (Chyb) enzyme encoding cDNA. Then, we completed the full sequencing of cDNA clone for its both strands using M13 forward and reverse primers. The full nucleotide and protein sequence was further analyzed and annotated using various Bioinformatics tools. The analysis results showed the presence of fatty acid hydroxylase superfamily domain in the protein sequence. The multiple sequence alignment of selected Chyb amino acid sequences from other plant species and algal members with E. oleifera Chyb using ClustalW and its phylogenetic analysis suggest that Chyb from monocotyledonous plant species, Lilium hubrid, Crocus sativus and Zea mays are the most evolutionary related with E. oleifera Chyb. This study reports the annotation of E. oleifera Chyb. Abbreviations ESTs - expressed sequence tags, EoChyb - Elaeis oleifera beta-carotene hydroxylase, MC - main cluster PMID:21364789

Loop propensity of the sequence YKGQP from staphylococcal nuclease: implications for the folding of nuclease.

PubMed

Patel, Sunita; Sasidhar, Yellamraju U

2007-10-01

Recently we performed molecular dynamics (MD) simulations on the folding of the hairpin peptide DTVKLMYKGQPMTFR from staphylococcal nuclease in explicit water. We found that the peptide folds into a hairpin conformation with native and nonnative hydrogen-bonding patterns. In all the folding events observed in the folding of the hairpin peptide, loop formation involving the region YKGQP was an important event. In order to trace the origins of the loop propensity of the sequence YKGQP, we performed MD simulations on the sequence starting from extended, polyproline II and native type I' turn conformations for a total simulation length of 300 ns, using the GROMOS96 force field under constant volume and temperature (NVT) conditions. The free-energy landscape of the peptide YKGQP shows minima corresponding to loop conformation with Tyr and Pro side-chain association, turn and extended conformational forms, with modest free-energy barriers separating the minima. To elucidate the role of Gly in facilitating loop formation, we also performed MD simulations of the mutated peptide YKAQP (Gly --> Ala mutation) under similar conditions starting from polyproline II conformation for 100 ns. Two minima corresponding to bend/turn and extended conformations were observed in the free-energy landscape for the peptide YKAQP. The free-energy barrier between the minima in the free-energy landscape of the peptide YKAQP was also modest. Loop conformation is largely sampled by the YKGQP peptide, while extended conformation is largely sampled by the YKAQP peptide. We also explain why the YKGQP sequence samples type II turn conformation in these simulations, whereas the sequence as part of the hairpin peptide DTVKLMYKGQPMTFR samples type I' turn conformation both in the X-ray crystal structure and in our earlier simulations on the folding of the hairpin peptide. We discuss the implications of our results to the folding of the staphylococcal nuclease. Copyright (c) 2007 European Peptide Society and John Wiley & Sons, Ltd.

Conformational analysis of the N-terminal sequence Met1 Val60 of the tyrosine hydroxylase

NASA Astrophysics Data System (ADS)

Alieva, Irada N.; Mustafayeva, Narmina N.; Gojayev, Niftali M.

2006-03-01

Molecular mechanics method and molecular dynamics (MD) simulation techniques are used to study the behavior and the effect of the amino acids substitution on structure and molecular dynamics of the specific portion of Met1-Val60 amino acid residues from N-terminal regulatory domain of the tyrosine hydroxylase (TH) and its mutants in which the positively charged arginine residues at positions 37 and 38 were replaced by electrically neutral Gly and negatively charged Glu, and serine residue at position 40 was replaced by Ala or Asp residue. Our study allowed us to make the following conclusions: (i) the higher conformational flexibility of the Met1-Arg16 sequence is revealed in comparision to other part of the N-terminus; (ii) the stretch of amino acid residues Met30-Ser40 within the N-terminus forms β-turn so that two α-helices (residues 16-29 and residues 41-60) are paralel one another; (ii) the significant differences that are observed for the Arg37→Gly37, Arg37-Arg38→Glu37-Glu38 mutant segments indicates that the positive charge of the Arg37 and Arg38 residues is one of the main factor that maintains the characteristic of the turn; (ii) no major conformational changes are observed between Ser40→Ala40, and Ser40→Asp40 mutant segments.

Expanding the peptide beta-turn in alphagamma hybrid sequences: 12 atom hydrogen bonded helical and hairpin turns.

PubMed

Chatterjee, Sunanda; Vasudev, Prema G; Raghothama, Srinivasarao; Ramakrishnan, Chandrasekharan; Shamala, Narayanaswamy; Balaram, Padmanabhan

2009-04-29

Hybrid peptide segments containing contiguous alpha and gamma amino acid residues can form C(12) hydrogen bonded turns which may be considered as backbone expanded analogues of C(10) (beta-turns) found in alphaalpha segments. Exploration of the regular hydrogen bonded conformations accessible for hybrid alphagamma sequences is facilitated by the use of a stereochemically constrained gamma amino acid residue gabapentin (1-aminomethylcyclohexaneacetic acid, Gpn), in which the two torsion angles about C(gamma)-C(beta) (theta(1)) and C(beta)-C(alpha) (theta(2)) are predominantly restricted to gauche conformations. The crystal structures of the octapeptides Boc-Gpn-Aib-Gpn-Aib-Gpn-Aib-Gpn-Aib-OMe (1) and Boc-Leu-Phe-Val-Aib-Gpn-Leu-Phe-Val-OMe (2) reveal two distinct conformations for the Aib-Gpn segment. Peptide 1 forms a continuous helix over the Aib(2)-Aib(6) segment, while the peptide 2 forms a beta-hairpin structure stabilized by four cross-strand hydrogen bonds with the Aib-Gpn segment forming a nonhelical C(12) turn. The robustness of the helix in peptide 1 in solution is demonstrated by NMR methods. Peptide 2 is conformationally fragile in solution with evidence of beta-hairpin conformations being obtained in methanol. Theoretical calculations permit delineation of the various C(12) hydrogen bonded structures which are energetically feasible in alphagamma and gammaalpha sequences.

Science Scope.

ERIC Educational Resources Information Center

Stone, Richard, Ed.

1995-01-01

Discusses an education project launched by the National Academy of Sciences and the Pentagon to turn laid-off aerospace engineers into science teachers at Los Angeles middle schools and high schools. (MKR)

Beta-hairpin formation in aqueous solution and in the presence of trifluoroethanol: a (1)H and (13)C nuclear magnetic resonance conformational study of designed peptides.

PubMed

Santiveri, Clara M; Pantoja-Uceda, David; Rico, Manuel; Jiménez, M Angeles

2005-10-15

In order to check our current knowledge on the principles involved in beta-hairpin formation, we have modified the sequence of a 3:5 beta-hairpin forming peptide with two different purposes, first to increase the stability of the formed 3:5 beta-hairpin, and second to convert the 3:5 beta-hairpin into a 2:2 beta-hairpin. The conformational behavior of the designed peptides was investigated in aqueous solution and in 30% trifluoroethanol (TFE) by analysis of the following nuclear magnetic resonance (NMR) parameters: nuclear Overhauser effect (NOE) data, and C(alpha)H, (13)C(alpha), and (13)C(beta) conformational shifts. From the differences in the ability to adopt beta-hairpin structures in these peptides, we have arrived to the following conclusions: (i) beta-Hairpin population increases with the statistical propensity of residues to occupy each turn position. (ii) The loop length, and in turn, the beta-hairpin type, can be modified as a function of the type of turn favored by the loop sequence. These two conclusions reinforce previous results about the importance of beta-turn sequence in beta-hairpin folding. (iii) Side-chain packing on each face of the beta-sheet may play a major role in beta-hairpin stability; hence simplified analysis in terms of isolated pair interactions and intrinsic beta-sheet propensities is insufficient. (iv) Contributions to beta-hairpin stability of turn and strand sequences are not completely independent. (v) The burial of hydrophobic surface upon beta-hairpin formation that, in turn, depends on side-chain packing also contributes to beta-hairpin stability. (vi) As previously observed, TFE stabilizes beta-hairpin structures, but the extent of the contribution of different factors to beta-hairpin formation is sometimes different in aqueous solution and in 30% TFE. (c) 2005 Wiley Periodicals, Inc. Biopolymers 79: 150-162, 2005.

Design and Test of Passively Operated Heat Switches for 0.2 to 15 K

NASA Technical Reports Server (NTRS)

DiPirro, M. J.; Shirron, P. J.; Canavan, E. R.; Francis, J. J.; Tuttle, J. G.

2003-01-01

Heat switches have many uses in cryogenics, from regulating heat flow between refrigeration stages to thermally isolating components once they have cooled to low temperature. Among the techniques one can use for thermal switching, the gas-gap technique has the advantages of wide operating temperature range, high switching ratio, and no moving parts. The traditional gas-gap switch uses copper conductors separated by a small gap and an external getter. The switch is activated by heating and cooling the getter by moving gas into and out of the gap, turning the switch on and off. We have designed, built and tested heat switches that use an internal getter to passively turn off at temperatures between 0.2 and 15 K. The getter is thermally anchored to one side of the switch, and when that side of the switch cools through a transition region, gas adsorbs onto the getter and the switch turns off. The challenges are to make the transition region very narrow and tailorable to a wide range of applications, and to achieve high gas conductance when the switch is on. We have made switches using He-3, He-4, hydrogen, and neon gas, and have used charcoal and various metal substrates as getters. Switching ratios range from 1000 to over 10,000. Design and performance of these switches will be discussed in detail.

Hyperhidrosis

MedlinePlus

... not right for everyone. Iontophoresis . This procedure uses electricity to temporarily turn off the sweat gland. It ... into water, and then a gentle current of electricity is passed through it. The electricity is gradually ...

36 CFR 1193.51 - Compatibility.

Code of Federal Regulations, 2010 CFR

2010-07-01

... BOARD TELECOMMUNICATIONS ACT ACCESSIBILITY GUIDELINES Requirements for Compatibility With Peripheral... user to easily turn any microphone on and off to allow the user to intermix speech with TTY use. (e...

36 CFR 1193.51 - Compatibility.

Code of Federal Regulations, 2011 CFR

2011-07-01

... BOARD TELECOMMUNICATIONS ACT ACCESSIBILITY GUIDELINES Requirements for Compatibility With Peripheral... user to easily turn any microphone on and off to allow the user to intermix speech with TTY use. (e...

Shelter

MedlinePlus

... room on the lowest level away from corners, windows, doors and outside walls. The safest locations to ... your family and pets inside. Lock doors, close windows, air vents and fireplace dampers. Turn off fans, ...

[Study of beta-turns in globular proteins].

PubMed

Amirova, S R; Milchevskiĭ, Iu V; Filatov, I V; Esipova, N G; Tumanian, V G

2005-01-01

The formation of beta-turns in globular proteins has been studied by the method of molecular mechanics. Statistical method of discriminant analysis was applied to calculate energy components and sequences of oligopeptide segments, and after this prediction of I type beta-turns has been drawn. The accuracy of true positive prediction is 65%. Components of conformational energy considerably affecting beta-turn formation were delineated. There are torsional energy, energy of hydrogen bonds, and van der Waals energy.

The Red Spiral Galaxy UGC11680: Clues for the Inside-Out Quenching.

NASA Astrophysics Data System (ADS)

Bárcenas, J.; Sanchez, S. F.

2016-06-01

Broadly, galaxies can be divided in two groups, thanks to the Color-Magnitude Diagram: the lively star formation ones, ``The blue Cloud'' and galaxies which halted their star formation, ``The Red Sequence''. It is a currently accepted that the galaxies start their lifespan as a blue objects, turning red when they stop to assembly more mass and thus more stars. Nevertheless, This change need to be quick (˜ 1 Gyr), due to the dearth of galaxies between this two populations (the so called ``green valley'').Previous works have found two distinct stellar mass assembly modes, they are termed as ``the inside-out'' and ``the outside-in'' growth scenarios in the literature. In the ``inside-out'' scenario, mass assembly is finished in the galactic central region. In some cases, the inflow gas can fuel the central SuperMassive BlackHole. The subsequent AGN feedback will then shut-off the central star formation. One possible case of this scenario is the galaxy UGC11680, an unusual face-on red spiral galaxy with an AGN type 2, at the red sequence belonging to the CALIFA survey. We used the so called fossil method to study its star formation history and try to understand what happened to its stellar populations.

Synthesis and Characterization of a Magnetically Active 19F Molecular Beacon.

PubMed

Dempsey, Megan E; Marble, Hetal D; Shen, Tun-Li; Fawzi, Nicolas L; Darling, Eric M

2018-02-21

Gene expression is used extensively to describe cellular characteristics and behaviors; however, most methods of assessing gene expression are unsuitable for living samples, requiring destructive processes such as fixation or lysis. Recently, molecular beacons have become a viable tool for live-cell imaging of mRNA molecules in situ. Historically, beacon-mediated imaging has been limited to fluorescence-based approaches. We propose the design and synthesis of a novel molecular beacon for magnetic resonance detection of any desired target nucleotide sequence. The biologically compatible synthesis incorporates commonly used bioconjugation reactions in aqueous conditions and is accessible for laboratories without extensive synthesis capabilities. The resulting beacon uses fluorine ( 19 F) as a reporter, which is broadened, or turned "off", via paramagnetic relaxation enhancement from a stabilized nitroxide radical spin label when the beacon is not bound to its nucleic acid target. Therefore, the 19 F NMR signal of the beacon is quenched in its hairpin conformation when the spin label and the 19 F substituent are held in proximity, but the signal is recovered upon beacon hybridization to its specific complementary nucleotide sequence by physical separation of the radical from the 19 F reporter. This study establishes a path for magnetic resonance-based assessment of specific mRNA expression, providing new possibilities for applying molecular beacon technology in living systems.

Generation of TALE-Based Designer Epigenome Modifiers.

PubMed

Nitsch, Sandra; Mussolino, Claudio

2018-01-01

Manipulation of gene expression can be facilitated by editing the genome or the epigenome. Precise genome editing is traditionally achieved by using designer nucleases which are generally exploited to eliminate a specific gene product. Upon the introduction of a site-specific DNA double-strand break (DSB) by the nuclease, endogenous DSB repair mechanisms are in turn harnessed to induce DNA sequence changes that can result in target gene inactivation. Minimal off-target effects can be obtained by endowing designer nucleases with the highly specific DNA-binding domain (DBD) derived from transcription activator-like effectors (TALEs). In contrast, epigenome editing allows gene expression control without inducing changes in the DNA sequence by specifically altering epigenetic marks, as histone tails modifications or DNA methylation patterns within promoter or enhancer regions. Importantly, this approach allows both up- and downregulation of the target gene expression, and the effect is generally reversible. TALE-based designer epigenome modifiers combine the high specificity of TALE-derived DBDs with the power of epigenetic modifier domains to induce fast and long-lasting changes in the epigenetic landscape of a target gene and control its expression. Here we provide a detailed description for the generation of TALE-based designer epigenome modifiers and of a suitable reporter cell line to easily monitor their activity.

Fast Regulation of Vertical Squat Jump during Push-Off in Skilled Jumpers

PubMed Central

Fargier, Patrick; Massarelli, Raphael; Rabahi, Tahar; Gemignani, Angelo; Fargier, Emile

2016-01-01

The height of a maximum Vertical Squat Jump (VSJ) reflects the useful power produced by a jumper during the push-off phase. In turn this partly depends on the coordination of the jumper's segmental rotations at each instant. The physical system constituted by the jumper has been shown to be very sensitive to perturbations and furthermore the movement is realized in a very short time (ca. 300 ms), compared to the timing of known feedback loops. However, the dynamics of the segmental coordination and its efficiency in relation to energetics at each instant of the push-off phase still remained to be clarified. Their study was the main purpose of the present research. Eight young adult volunteers (males) performed maximal VSJ. They were skilled in jumping according to their sport activities (track and field or volleyball). A video analysis on the kinematics of the jump determined the influence of the jumpers' segments rotation on the vertical velocity and acceleration of the body mass center (MC). The efficiency in the production of useful power at the jumpers' MC level, by the rotation of the segments, was measured in consequence. The results showed a great variability in the segmental movements of the eight jumpers, but homogeneity in the overall evolution of these movements with three consecutive types of coordination in the second part of the push-off (lasting roughly 0.16 s). Further analyses gave insights on the regulation of the push-off, suggesting that very fast regulation(s) of the VSJ may be supported by: (a) the adaptation of the motor cerebral programming to the jumper's physical characteristics; (b) the control of the initial posture; and (c) the jumper's perception of the position of his MC relative to the ground reaction force, during push-off, to reduce energetic losses. PMID:27486404

CPR (For Parents)

MedlinePlus

... or she is no longer in contact with electricity before offering assistance to prevent becoming electrocuted yourself. (For instance, turn off the source of electricity, such as a light switch or a circuit ...

Conceptual Chemistry: Understanding Our World of Atoms and Molecules (John Suchocki)

NASA Astrophysics Data System (ADS)

Pienta, Norbert J.

2003-07-01

In summary, this book defines its philosophy and sticks to it. It is well-written and will be understandable to the nonscience major; it has a rational organization and layout. The core follows the principles covered in most general chemistry courses. For the nonscience-major audience, it would be challenging to cover the core and very many of the enrichment chapters, but there is certainly plenty to choose from in both categories. For the prep-chem audience, the relatively small amount of mathematics, few calculations, and implementation may turn off instructors. (But perhaps that is why students in those courses are turned off by the traditional textbooks that are offered for their use.) If you are in the market in this or a related category, give this book a serious look.

[Y/Mg] stellar dating calibration

NASA Astrophysics Data System (ADS)

Titarenko, A.; Recio-Blanco, A.; de Laverny, P.; Hayden, M.; Guiglion, G.; Worley, C.

2018-04-01

Gaia DR1 has opened a new era of stellar age dating, that is crucial for many astrophysical objectives. In addition, the Gaia based isochrone fitting ages can be compared to other chemical clocks like the [Y/Mg] one (Nissen et al. 2015). In our work we have used ESO archived data of the AMBRE project (de Laverny et al. 2013) for UVES spectra, in order to evaluate the age [Y/Mg] abundance correlation for turn off stars. 310 turn off stars of the UVES-archive (setups 564 and 580) are included in the TGAS database. Isochrone fitting ages were derived. We have applied the GAUGUIN procedure for those stars to derive the Mg and the Y abundances. As the result we present the [Y/Mg] vs stellar age dependence for ~40 TO-stars.

Dependence of regenerated sensory axons on continuous neurotrophin-3 delivery.

PubMed

Hou, Shaoping; Nicholson, LaShae; van Niekerk, Erna; Motsch, Melanie; Blesch, Armin

2012-09-19

Previous studies have shown that injured dorsal column sensory axons extend across a spinal cord lesion site if axons are guided by a gradient of neurotrophin-3 (NT-3) rostral to the lesion. Here we examined whether continuous NT-3 delivery is necessary to sustain regenerated axons in the injured spinal cord. Using tetracycline-regulated (tet-off) lentiviral gene delivery, NT-3 expression was tightly controlled by doxycycline administration. To examine axon growth responses to regulated NT-3 expression, adult rats underwent a C3 dorsal funiculus lesion. The lesion site was filled with bone marrow stromal cells, tet-off-NT-3 virus was injected rostral to the lesion site, and the intrinsic growth capacity of sensory neurons was activated by a conditioning lesion. When NT-3 gene expression was turned on, cholera toxin β-subunit-labeled sensory axons regenerated into and beyond the lesion/graft site. Surprisingly, the number of regenerated axons significantly declined when NT-3 expression was turned off, whereas continued NT-3 expression sustained regenerated axons. Quantification of axon numbers beyond the lesion demonstrated a significant decline of axon growth in animals with transient NT-3 expression, only some axons that had regenerated over longer distance were sustained. Regenerated axons were located in white matter and did not form axodendritic synapses but expressed presynaptic markers when closely associated with NG2-labeled cells. A decline in axon density was also observed within cellular grafts after NT-3 expression was turned off possibly via reduction in L1 and laminin expression in Schwann cells. Thus, multiple mechanisms underlie the inability of transient NT-3 expression to fully sustain regenerated sensory axons.

The evolution of surface magnetic fields in young solar-type stars II: the early main sequence (250-650 Myr)

NASA Astrophysics Data System (ADS)

Folsom, C. P.; Bouvier, J.; Petit, P.; Lèbre, A.; Amard, L.; Palacios, A.; Morin, J.; Donati, J.-F.; Vidotto, A. A.

2018-03-01

There is a large change in surface rotation rates of sun-like stars on the pre-main sequence and early main sequence. Since these stars have dynamo-driven magnetic fields, this implies a strong evolution of their magnetic properties over this time period. The spin-down of these stars is controlled by interactions between stellar and magnetic fields, thus magnetic evolution in turn plays an important role in rotational evolution. We present here the second part of a study investigating the evolution of large-scale surface magnetic fields in this critical time period. We observed stars in open clusters and stellar associations with known ages between 120 and 650 Myr, and used spectropolarimetry and Zeeman Doppler Imaging to characterize their large-scale magnetic field strength and geometry. We report 15 stars with magnetic detections here. These stars have masses from 0.8 to 0.95 M⊙, rotation periods from 0.326 to 10.6 d, and we find large-scale magnetic field strengths from 8.5 to 195 G with a wide range of geometries. We find a clear trend towards decreasing magnetic field strength with age, and a power law decrease in magnetic field strength with Rossby number. There is some tentative evidence for saturation of the large-scale magnetic field strength at Rossby numbers below 0.1, although the saturation point is not yet well defined. Comparing to younger classical T Tauri stars, we support the hypothesis that differences in internal structure produce large differences in observed magnetic fields, however for weak-lined T Tauri stars this is less clear.

Translations on USSR Military Affairs, Number 1386

DTIC Science & Technology

1978-10-16

defended; sometimes they are com- pletely bare. They must be used to penetrate boldly to the depth of the defense. When fighting for control of...stopping the fans and supercharger , turning off the electric drive of the turret, and switching on the absorbent filter air delivery system. After...the shock wave passes the mechanic-driver should turn on the supercharger , which provides delivery of decontaminated air at sur- plus pressure into

Switching transients in high-frequency high-power converters using power MOSFET's

NASA Technical Reports Server (NTRS)

Sloane, T. H.; Owen, H. A., Jr.; Wilson, T. G.

1979-01-01

The use of MOSFETs in a high-frequency high-power dc-to-dc converter is investigated. Consideration is given to the phenomena associated with the paralleling of MOSFETs and to the effect of stray circuit inductances on the converter circuit performance. Analytical relationships between various time constants during the turning-on and turning-off intervals are derived which provide estimates of plateau and peak levels during these intervals.

Phocine Distemper Virus in Seals, East Coast, United States, 2006

PubMed Central

Earle, J.A. Philip; Melia, Mary M.; Doherty, Nadine V.; Nielsen, Ole

2011-01-01

In 2006 and 2007, elevated numbers of deaths among seals, constituting an unusual mortality event, occurred off the coasts of Maine and Massachusetts, United States. We isolated a virus from seal tissue and confirmed it as phocine distemper virus (PDV). We compared the viral hemagglutinin, phosphoprotein, and fusion (F) and matrix (M) protein gene sequences with those of viruses from the 1988 and 2002 PDV epizootics. The virus showed highest similarity with a PDV 1988 Netherlands virus, which raises the possibility that the 2006 isolate from the United States might have emerged independently from 2002 PDVs and that multiple lineages of PDV might be circulating among enzootically infected North American seals. Evidence from comparison of sequences derived from different tissues suggested that mutations in the F and M genes occur in brain tissue that are not present in lung, liver, or blood, which suggests virus persistence in the central nervous system. PMID:21291591

Improving hand functional use in subjects with multiple sclerosis using a musical keyboard: a randomized controlled trial.

PubMed

Gatti, Roberto; Tettamanti, Andrea; Lambiase, Simone; Rossi, Paolo; Comola, Mauro

2015-06-01

Playing an instrument implies neuroplasticity in different cerebral regions. This phenomenon has been described in subjects with stroke, suggesting that it could play a role in hand rehabilitation. The aim of this study is to analyse the effectiveness of playing a musical keyboard in improving hand function in subjects with multiple sclerosis. Nineteen hospitalized subjects were randomized in two groups: nine played a turned-on musical keyboard by sequences of fingers movements (audio feedback present) and 10 performed the same exercises on a turned-off musical keyboard (audio feedback absent). Training duration was half an hour per day for 15 days. Primary outcome was the perceived hand functional use measured by ABILHAND Questionnaire. Secondary outcomes were hand dexterity, measured by Nine-Hole Peg Test, and hand strength, measured by Jamar and Pinch dynamometers. Two-way analysis of variance was used for data analysis. The interaction time × group was significant (p = 0.003) for ABILHAND Questionnaire in favour of experimental group (mean between-group difference 0.99 logit [IC95%: 0.44; 1.54]). The two groups showed a significant time effect for all outcomes except for Jamar measure. Playing a musical keyboard seems a valid method to train the functional use of hands in subjects with multiple sclerosis. Copyright © 2014 John Wiley & Sons, Ltd.

Characteristics of dune-paleosol-sequences in Fuerteventura. - What should be questioned?

NASA Astrophysics Data System (ADS)

Faust, Dominik; Willkommen, Tobias; Yanes, Yurena; Richter, David; Zöller, Ludwig

2013-04-01

Characteristics of dune-paleosol-sequences in Fuerteventura. - What should be questioned? Dominik Faust, TU Dresden, Germany Tobias Willkommen, TU Dresden, Germany Yurena Yanes, CSIC Granada/Cincinatti, Spain/USA David Richter, TU Dresden, Germany Ludwig Zöller, Uni Bayreuth, Germany The northern part of Fuerteventura is characterized by large dune fields. We investigated dune-paleosol-sequences in four pits to establish a robust stratigraphy and to propose a standard section. An interaction of processes like dune formation, soil formation and redeposition of soils and sand are most important to understand the principles of landscape development in the study area. To our mind a process cycle seem to be important: First climbing-dunes are formed by sand of shelf origin. Then soil formation could have taken place. Soil and/or sand were then eroded and deposited at toe slope position. This material in turn is the source of new sand supply and dune formation. The described cycle may be repeated several times and this ping-pong-process holds on. The results are sections composed of dune layers, paleosols and colluvial material interbedded. Fundamental questions still remain unanswered: Is climate change responsable for changes in process combination (e.g. from dune formation to soil formation)? Or are these features due to divergence phenomenon, where different effects/results (dune and soils) may be linked to similar causes (here: climate)? Assuming that different features (soils and dunes) were formed under one climate, increasing soil forming intensity could be mainly a function of decreasing sand supply. This in turn could be caused by reduced sand production (s. ZECH et al. accepted). However geochemical data and mollusc assemblages point to changing environments in space and even climate modifications in time.

Emission projections of the transport Sector in China: 2015-2040

NASA Astrophysics Data System (ADS)

Yan, L.

2016-12-01

Driven by the significant growth freight and passenger transport demand, transport sector has become a sector that is largely responsible for increases in emissions of atmospheric pollutants (NOx, CO, HC, PM2.5) in China. Figuring out the emission trend in China's transport sector has great influence on formulating emission reduction measures in the future. In this work, both on-road and off-road transport emissions in China were estimated from 2015 to 2040 for CO, NOx, HC and PM2.5. The projection was conducted based on on the energy consumption structure forecast from IEA (International Energy Agency), the future national average annual distance traveled per vehicle and fuel consumption per distance derived from simulation results of the Fuel Economy and Environmental Impact (FEEI) model. The results show that the ownership of on-road vehicles in China increases rapidly during 2015 to 2030 and then the growth slows down. Finally, the total amount reaches up to 522 million in 2040 in which 84.5% turns out to be light-duty vehicles. Because current control legislations for the transport sector in China will continue to be strengthened in the future, the total emissions of China's transport sector were projected to peak around 2030, due to the improvement of vehicle emission standard and the retirement of old vehicles are the most effective measures. The off-road transport will become the main contributor to emissions from transport sector in China since 2030. This work provides a new perspective to understand emissions from both on-road and off-road transport in China, which can support the achievement of improving air quality promised by the Chinese government. This work provides a new perspective to understand the emission trends of on-road and off-road transport sector in China from 2015 to 2040, which can support the achievement of the air quality goal promised by the Chinese government. Driven by the significant growth freight and passenger transport demand, transport sector has become a sector that is largely responsible for increases in emissions of atmospheric pollutants (NOx, CO, HC, PM2.5) in China. Figuring out the emission trend in China's transport sector has great influence on formulating emission reduction measures in the future. In this work, both on-road and off-road transport emissions in China were estimated from 2015 to 2040 for CO, NOx, HC and PM2.5. The projection was conducted based on on the energy consumption structure forecast from IEA (International Energy Agency), the future national average annual distance traveled per vehicle and fuel consumption per distance derived from simulation results of the Fuel Economy and Environmental Impact (FEEI) model. The results show that the ownership of on-road vehicles in China increases rapidly during 2015 to 2030 and then the growth slows down. Finally, the total amount reaches up to 522 million in 2040 in which 84.5% turns out to be light-duty vehicles. Because current control legislations for the transport sector in China will continue to be strengthened in the future, the total emissions of China's transport sector were projected to peak around 2030, due to the improvement of vehicle emission standard and the retirement of old vehicles are the most effective measures. The off-road transport will become the main contributor to emissions from transport sector in China since 2030. This work provides a new perspective to understand emissions from both on-road and off-road transport in China, which can support the achievement of improving air quality promised by the Chinese government. This work provides a new perspective to understand the emission trends of on-road and off-road transport sector in China from 2015 to 2040, which can support the achievement of the air quality goal promised by the Chinese government.

Off-Site Indexing: A Cottage Industry.

ERIC Educational Resources Information Center

Fay, Catherine H.

1984-01-01

Briefly describes use of off-site staffing--indexers, abstractors, editors--in the production of two major databases: Management Contents and The Computer Data Base. Discussion covers the production sequence; database administrator; off-site indexer; savings (office space, furniture and equipment costs, salaries, and overhead); and problems…

Copper deficiency alters cell bioenergetics and induces mitochondrial fusion through up-regulation of MFN2 and OPA1 in erythropoietic cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bustos, Rodrigo I.; Jensen, Erik L.; Ruiz, Lina M.

2013-08-02

Highlights: •In copper deficiency, cell proliferation is not affected. In turn, cell differentiation is impaired. •Enlarged mitochondria are due to up-regulation of MNF2 and OPA1. •Mitochondria turn off respiratory chain and ROS production. •Energy metabolism switch from mitochondria to glycolysis. -- Abstract: Copper is essential in cell physiology, participating in numerous enzyme reactions. In mitochondria, copper is a cofactor for respiratory complex IV, the cytochrome c oxidase. Low copper content is associated with anemia and the appearance of enlarged mitochondria in erythropoietic cells. These findings suggest a connection between copper metabolism and bioenergetics, mitochondrial dynamics and erythropoiesis, which has notmore » been explored so far. Here, we describe that bathocuproine disulfonate-induced copper deficiency does not alter erythropoietic cell proliferation nor induce apoptosis. However it does impair erythroid differentiation, which is associated with a metabolic switch between the two main energy-generating pathways. That is, from mitochondrial function to glycolysis. Switching off mitochondria implies a reduction in oxygen consumption and ROS generation along with an increase in mitochondrial membrane potential. Mitochondrial fusion proteins MFN2 and OPA1 were up-regulated along with the ability of mitochondria to fuse. Morphometric analysis of mitochondria did not show changes in total mitochondrial biomass but rather bigger mitochondria because of increased fusion. Similar results were also obtained with human CD34+, which were induced to differentiate into red blood cells. In all, we have shown that adequate copper levels are important for maintaining proper mitochondrial function and for erythroid differentiation where the energy metabolic switch plus the up-regulation of fusion proteins define an adaptive response to copper deprivation to keep cells alive.« less

The Development of Smart Home System for Controlling and Monitoring Energy Consumption using WebSocket Protocol

NASA Astrophysics Data System (ADS)

Witthayawiroj, Niti; Nilaphruek, Pongpon

2017-03-01

Energy consumption especially electricity is considered one of the most serious problems in households these days. It is because the amount of electricity consumed is more than the amount that people actually need. This means that there is an overusing which resulted from the inconvenience of moving to the switch to turn off the light or any appliances and it is often that closing the light is forgettable, for instance; in addition, there are no tools for monitoring how much energy that is consumed in residents. From this, it can be easily seen that people are having a problem in energy usage monitor and control. There are two main objectives of this study including 1) creating the communication framework among server, clients and devices, and 2) developing the prototype system that try to solve the mentioned problems which gives the user an opportunity to know the amount of electricity they have used in their houses and also the ability to turn appliances on and off through the Internet on smart devices such as smart phones and tablets that support Android platform or any web browser. Raspberry Pi is used as a microcontroller and the data is transferred to the smart device by WebSocket protocol which is strongly recommended for real-time communication. The example features on the device’s screen are user management, controlling and monitoring of appliances. The result expresses that the system is very effective and not difficult to use from users’ satisfaction. However, current sensors may be used for a more accurate electricity measurement and Wi-Fi module for more appliances to calculate its power in the future.

LC-lens array with light field algorithm for 3D biomedical applications

NASA Astrophysics Data System (ADS)

Huang, Yi-Pai; Hsieh, Po-Yuan; Hassanfiroozi, Amir; Martinez, Manuel; Javidi, Bahram; Chu, Chao-Yu; Hsuan, Yun; Chu, Wen-Chun

2016-03-01

In this paper, liquid crystal lens (LC-lens) array was utilized in 3D bio-medical applications including 3D endoscope and light field microscope. Comparing with conventional plastic lens array, which was usually placed in 3D endoscope or light field microscope system to record image disparity, our LC-lens array has higher flexibility of electrically changing its focal length. By using LC-lens array, the working distance and image quality of 3D endoscope and microscope could be enhanced. Furthermore, the 2D/3D switching ability could be achieved if we turn off/on the electrical power on LClens array. In 3D endoscope case, a hexagonal micro LC-lens array with 350um diameter was placed at the front end of a 1mm diameter endoscope. With applying electric field on LC-lens array, the 3D specimen would be recorded as from seven micro-cameras with different disparity. We could calculate 3D construction of specimen with those micro images. In the other hand, if we turn off the electric field on LC-lens array, the conventional high resolution 2D endoscope image would be recorded. In light field microscope case, the LC-lens array was placed in front of the CMOS sensor. The main purpose of LC-lens array is to extend the refocusing distance of light field microscope, which is usually very narrow in focused light field microscope system, by montaging many light field images sequentially focusing on different depth. With adjusting focal length of LC-lens array from 2.4mm to 2.9mm, the refocusing distance was extended from 1mm to 11.3mm. Moreover, we could use a LC wedge to electrically shift the optics axis and increase the resolution of light field.

Local sequence information in cellular retinoic acid-binding protein I: specific residue roles in beta-turns.

PubMed

Rotondi, Kenneth S; Gierasch, Lila M

2003-01-01

We have recently shown that two of the beta-turns (III and IV) in the ten-stranded, beta-clam protein, cellular retinoic acid-binding protein I (CRABP I), are favored in short peptide fragments, arguing that they are encoded by local interactions (K. S. Rotondi and L. M. Gierasch, Biochemistry, 2003, Vol. 42, pp. 7976-7985). In this paper we examine these turns in greater detail to dissect the specific local interactions responsible for their observed native conformational biases. Conformations of peptides corresponding to the turn III and IV fragments were examined under conditions designed to selectively disrupt stabilizing interactions, using pH variation, chaotrope addition, or mutagenesis to probe specific side-chain influences. We find that steric constraints imposed by excluded volume effects between near neighbor residues (i,i+2), favorable polar (i,i+2) interactions, and steric permissiveness of glycines are the principal factors accounting for the observed native bias in these turns. Longer-range stabilizing interactions across the beta-turns do not appear to play a significant role in turn stability in these short peptides, in contrast to their importance in hairpins. Additionally, our data add to a growing number of examples of the 3:5 type I turn with a beta-bulge as a class of turns with high propensity to form locally defined structure. Current work is directed at the interplay between the local sequence information in the turns and more long-range influences in the mechanism of folding of this predominantly beta-sheet protein. Copyright 2004 Wiley Periodicals, Inc.

Mold contamination of automobile air conditioner systems.

PubMed

Kumar, P; Lopez, M; Fan, W; Cambre, K; Elston, R C

1990-02-01

Eight cars belonging to patients who were found to have exacerbation of allergic rhinitis and bronchial asthma after turning on the air conditioner in their cars were examined. Mold concentrations inside the passenger compartment with the a/c turned off and at different climate control settings were lower than concentrations in the outside air. After turning on the air conditioner to "Max", cultures obtained at various intervals revealed that mold concentrations decreased significantly with time. Furthermore, placement of a filter at the portal of entry of outside air significantly reduced the mold concentration in the passenger compartment.

33 CFR 150.615 - What safe practices are required?

Code of Federal Regulations, 2010 CFR

2010-07-01

... work they perform, including the use of electrical personal protective equipment appropriate to protect... not feasible. (b) The deepwater port operator must ensure that personnel turning off equipment...

33 CFR 150.615 - What safe practices are required?

Code of Federal Regulations, 2011 CFR

2011-07-01

... work they perform, including the use of electrical personal protective equipment appropriate to protect... not feasible. (b) The deepwater port operator must ensure that personnel turning off equipment...

pi-Turns: types, systematics and the context of their occurrence in protein structures

PubMed Central

Dasgupta, Bhaskar; Chakrabarti, Pinak

2008-01-01

Background For a proper understanding of protein structure and folding it is important to know if a polypeptide segment adopts a conformation inherent in the sequence or it depends on the context of its flanking secondary structures. Turns of various lengths have been studied and characterized starting from three-residue γ-turn to six-residue π-turn. The Schellman motif occurring at the C-terminal end of α-helices is a classical example of hydrogen bonded π-turn involving residues at (i) and (i+5) positions. Hydrogen bonded and non-hydrogen bonded β- and α-turns have been identified previously; likewise, a systematic characterization of π-turns would provide valuable insight into turn structures. Results An analysis of protein structures indicates that at least 20% of π-turns occur independent of the Schellman motif. The two categories of π-turns, designated as π-HB and SCH, have been further classified on the basis of backbone conformation and both have AAAa as the major class. They differ in the residue usage at position (i+1), the former having a large preference for Pro that is absent in the latter. As in the case of shorter length β- and α-turns, π-turns have also been identified not only on the basis of the existence of hydrogen bond, but also using the distance between terminal Cα-atoms, and this resulted in a comparable number of non-hydrogen-bonded π-turns (π-NHB). The presence of shorter β- and α-turns within all categories of π-turns, the subtle variations in backbone torsion angles along the turn residues, the location of the turns in the context of tertiary structures have been studied. Conclusion π-turns have been characterized, first using hydrogen bond and the distance between Cα atoms of the terminal residues, and then using backbone torsion angles. While the Schellman motif has a structural role in helix termination, many of the π-HB turns, being located on surface cavities, have functional role and there is also sequence conservation. PMID:18808671

pi-Turns: types, systematics and the context of their occurrence in protein structures.

PubMed

Dasgupta, Bhaskar; Chakrabarti, Pinak

2008-09-22

For a proper understanding of protein structure and folding it is important to know if a polypeptide segment adopts a conformation inherent in the sequence or it depends on the context of its flanking secondary structures. Turns of various lengths have been studied and characterized starting from three-residue gamma-turn to six-residue pi-turn. The Schellman motif occurring at the C-terminal end of alpha-helices is a classical example of hydrogen bonded pi-turn involving residues at (i) and (i+5) positions. Hydrogen bonded and non-hydrogen bonded beta- and alpha-turns have been identified previously; likewise, a systematic characterization of pi-turns would provide valuable insight into turn structures. An analysis of protein structures indicates that at least 20% of pi-turns occur independent of the Schellman motif. The two categories of pi-turns, designated as pi-HB and SCH, have been further classified on the basis of backbone conformation and both have AAAa as the major class. They differ in the residue usage at position (i+1), the former having a large preference for Pro that is absent in the latter. As in the case of shorter length beta- and alpha-turns, pi-turns have also been identified not only on the basis of the existence of hydrogen bond, but also using the distance between terminal C alpha-atoms, and this resulted in a comparable number of non-hydrogen-bonded pi-turns (pi-NHB). The presence of shorter beta- and alpha-turns within all categories of pi-turns, the subtle variations in backbone torsion angles along the turn residues, the location of the turns in the context of tertiary structures have been studied. pi-turns have been characterized, first using hydrogen bond and the distance between C alpha atoms of the terminal residues, and then using backbone torsion angles. While the Schellman motif has a structural role in helix termination, many of the pi-HB turns, being located on surface cavities, have functional role and there is also sequence conservation.

Supply and demand of timber for wood turning in Maine

Treesearch

Eric H. Wharton; Robert L., Jr. Nevel; Douglas S. Powell; Douglas S. Powell

1987-01-01

An analytical report on the volume of wood used by the wood-turning industry in Maine, and the volume of timber from the state's timberlands that may be suitable for turnstock. Findings are based on the third forest resource survey of Maine timberlands, and an industry canvass of primary manufacturing mills using wood from Maine timberlands, both conducted in 1982...

Hydrogen Bonded Squaramide-Based Foldable Module Induces Both β- and α-Turns in Hairpin Structures of α-Peptides in Water.

PubMed

Martínez, Luís; Martorell, Gabriel; Sampedro, Ángel; Ballester, Pablo; Costa, Antoni; Rotger, Carmen

2015-06-19

A novel tertiary squaramido-based reverse-turn module SQ is reported, and its conformational properties are evaluated. This module is easily incorporated into a α-peptide sequence by conventional solid-phase peptide synthesis. The structure characterization of the hybrid squaramido-peptide 4 is described, showing that the turn segment induces the formation of hairpin structures in water through the formation of both αSQ- and βSQ-turns.

Fluorescent signatures for variable DNA sequences

PubMed Central

Rice, John E.; Reis, Arthur H.; Rice, Lisa M.; Carver-Brown, Rachel K.; Wangh, Lawrence J.

2012-01-01

Life abounds with genetic variations writ in sequences that are often only a few hundred nucleotides long. Rapid detection of these variations for identification of genetic diseases, pathogens and organisms has become the mainstay of molecular science and medicine. This report describes a new, highly informative closed-tube polymerase chain reaction (PCR) strategy for analysis of both known and unknown sequence variations. It combines efficient quantitative amplification of single-stranded DNA targets through LATE-PCR with sets of Lights-On/Lights-Off probes that hybridize to their target sequences over a broad temperature range. Contiguous pairs of Lights-On/Lights-Off probes of the same fluorescent color are used to scan hundreds of nucleotides for the presence of mutations. Sets of probes in different colors can be combined in the same tube to analyze even longer single-stranded targets. Each set of hybridized Lights-On/Lights-Off probes generates a composite fluorescent contour, which is mathematically converted to a sequence-specific fluorescent signature. The versatility and broad utility of this new technology is illustrated in this report by characterization of variant sequences in three different DNA targets: the rpoB gene of Mycobacterium tuberculosis, a sequence in the mitochondrial cytochrome C oxidase subunit 1 gene of nematodes and the V3 hypervariable region of the bacterial 16 s ribosomal RNA gene. We anticipate widespread use of these technologies for diagnostics, species identification and basic research. PMID:22879378

Information and redundancy in the burial folding code of globular proteins within a wide range of shapes and sizes.

PubMed

Ferreira, Diogo C; van der Linden, Marx G; de Oliveira, Leandro C; Onuchic, José N; de Araújo, Antônio F Pereira

2016-04-01

Recent ab initio folding simulations for a limited number of small proteins have corroborated a previous suggestion that atomic burial information obtainable from sequence could be sufficient for tertiary structure determination when combined to sequence-independent geometrical constraints. Here, we use simulations parameterized by native burials to investigate the required amount of information in a diverse set of globular proteins comprising different structural classes and a wide size range. Burial information is provided by a potential term pushing each atom towards one among a small number L of equiprobable concentric layers. An upper bound for the required information is provided by the minimal number of layers L(min) still compatible with correct folding behavior. We obtain L(min) between 3 and 5 for seven small to medium proteins with 50 ≤ Nr ≤ 110 residues while for a larger protein with Nr = 141 we find that L ≥ 6 is required to maintain native stability. We additionally estimate the usable redundancy for a given L ≥ L(min) from the burial entropy associated to the largest folding-compatible fraction of "superfluous" atoms, for which the burial term can be turned off or target layers can be chosen randomly. The estimated redundancy for small proteins with L = 4 is close to 0.8. Our results are consistent with the above-average quality of burial predictions used in previous simulations and indicate that the fraction of approachable proteins could increase significantly with even a mild, plausible, improvement on sequence-dependent burial prediction or on sequence-independent constraints that augment the detectable redundancy during simulations. © 2016 Wiley Periodicals, Inc.

Development of an Outdoor Temperature-Based Control Algorithm for Residential Mechanical Ventilation Control

DOE Office of Scientific and Technical Information (OSTI.GOV)

Less, Brennan; Walker, Iain; Tang, Yihuan

2014-06-01

Smart ventilation systems use controls to ventilate more during those periods that provide either an energy or IAQ advantage (or both) and less during periods that provide a dis advantage. Using detailed building simulations, this study addresses one of the simplest and lowest cost types of smart controllers —outdoor temperature- based control. If the outdoor temperature falls below a certain cut- off, the fan is simply turned off. T he main principle of smart ventilation used in this study is to shift ventilation from time periods with large indoor -outdoor temperature differences, to periods where these differences are smaller, andmore » their energy impacts are expected to be less. Energy and IAQ performance are assessed relative to a base case of a continuously operated ventilation fan sized to comply with ASHRAE 62.2-2013 whole house ventilation requirements. In order to satisfy 62.2-2013, annual pollutant exposure must be equivalent between the temperature controlled and continuous fan cases. This requires ventilation to be greater than 62.2 requirements when the ventilation system operates. This is achieved by increasing the mechanical ventilation system air flow rates.« less

The Zero Boil-Off Tank Experiment Ground Testing and Verification of Fluid and Thermal Performance

NASA Technical Reports Server (NTRS)

Chato, David J.; Kassemi, Mohammad; Kahwaji, Michel; Kieckhafer, Alexander

2016-01-01

The Zero Boil-Off Technology (ZBOT) Experiment involves performing a small scale International Space Station (ISS) experiment to study tank pressurization and pressure control in microgravity. The ZBOT experiment consists of a vacuum jacketed test tank filled with an inert fluorocarbon simulant liquid. Heaters and thermo-electric coolers are used in conjunction with an axial jet mixer flow loop to study a range of thermal conditions within the tank. The objective is to provide a high quality database of low gravity fluid motions and thermal transients which will be used to validate Computational Fluid Dynamic (CFD) modeling. This CFD can then be used in turn to predict behavior in larger systems with cryogens. This paper will discuss the work that has been done to demonstrate that the ZBOT experiment is capable of performing the functions required to produce a meaningful and accurate results, prior to its launch to the International Space Station. Main systems discussed are expected to include the thermal control system, the optical imaging system, and the tank filling system.This work is sponsored by NASAs Human Exploration Mission Directorates Physical Sciences Research program.

The Lesser Role of Starbursts in Star Formation at z = 2

NASA Astrophysics Data System (ADS)

Rodighiero, G.; Daddi, E.; Baronchelli, I.; Cimatti, A.; Renzini, A.; Aussel, H.; Popesso, P.; Lutz, D.; Andreani, P.; Berta, S.; Cava, A.; Elbaz, D.; Feltre, A.; Fontana, A.; Förster Schreiber, N. M.; Franceschini, A.; Genzel, R.; Grazian, A.; Gruppioni, C.; Ilbert, O.; Le Floch, E.; Magdis, G.; Magliocchetti, M.; Magnelli, B.; Maiolino, R.; McCracken, H.; Nordon, R.; Poglitsch, A.; Santini, P.; Pozzi, F.; Riguccini, L.; Tacconi, L. J.; Wuyts, S.; Zamorani, G.

2011-10-01

Two main modes of star formation are know to control the growth of galaxies: a relatively steady one in disk-like galaxies, defining a tight star formation rate (SFR)-stellar mass sequence, and a starburst mode in outliers to such a sequence which is generally interpreted as driven by merging. Such starburst galaxies are rare but have much higher SFRs, and it is of interest to establish the relative importance of these two modes. PACS/Herschel observations over the whole COSMOS and GOODS-South fields, in conjunction with previous optical/near-IR data, have allowed us to accurately quantify for the first time the relative contribution of the two modes to the global SFR density in the redshift interval 1.5 < z < 2.5, i.e., at the cosmic peak of the star formation activity. The logarithmic distributions of galaxy SFRs at fixed stellar mass are well described by Gaussians, with starburst galaxies representing only a relatively minor deviation that becomes apparent for SFRs more than four times higher than on the main sequence. Such starburst galaxies represent only 2% of mass-selected star-forming galaxies and account for only 10% of the cosmic SFR density at z ~ 2. Only when limited to SFR > 1000 M sun yr-1, off-sequence sources significantly contribute to the SFR density (46% ± 20%). We conclude that merger-driven starbursts play a relatively minor role in the formation of stars in galaxies, whereas they may represent a critical phase toward the quenching of star formation and morphological transformation in galaxies.

Cloning and expression analysis of a gene that shows developmental regulation upon tuberization in potato.

PubMed

Jackson, S; Gascón, J; Carrera, E; Monte, E; Prat, S

1997-01-01

Differential screening of a potato leaf cDNA library with cDNA probes made from tuberizing and non-tuberizing Solanum demissum plants led to the identification of a clone that is upregulated in leaves and other tissues upon tuberization. This clone was also shown to have a high level of expression in green tomato fruit, its expression falling off as the fruit turns red. No sucrose or hormonal regulation of the expression of this clone was observed and it did not respond to wounding or heat stress. Clone 32B is 532 bp long and contains an open reading frame encoding a small protein of 98 amino acids. The deduced protein sequence has a putative signal peptide for ER transport and a 10 amino acid domain in the C-terminal region of the protein, both of which are also found in the cotton LEA5, Arabidopsis Di21 and the mungbean Arg2 proteins.

Epigenetics, Media Coverage, and Parent Responsibilities in the Post-Genomic Era.

PubMed

Lappé, Martine

2016-09-01

Environmental epigenetics is the study of how exposures and experiences can turn genes "on" or "off" without changing DNA sequence. By examining the influence that environmental conditions including diet, stress, trauma, toxins, and care can have on gene expression, this science suggests molecular connections between the environment, genetics, and how acquired characteristics may be inherited across generations. The rapid expansion of research in this area has attracted growing media attention. This coverage has implications for how parents and prospective parents understand health and their perceived responsibilities for children's wellbeing. This review provides insight into epigenetic research, its coverage in the media, and the social and ethical implications of this science for patients and clinicians. As epigenetic findings continue to elucidate the complex relationships between nature and nurture, it becomes critical to examine how representations of this science may influence patient experiences of risk and responsibility. This review describes some of the social and ethical implications of epigenetic research today.

A Surveillance and Targeting System for an Unmanned Ground Vehicle

DTIC Science & Technology

1990-08-01

CHARACTERISTICS - SELECTABLE INFRASONIC AND ULTRASONIC FREQUENCY SHIFTING CAPABILITY - SUPER-BINAURAL CONFIGURATION ANGLE AND PICKUP SEPARATION GREATER THAN...HUMAN HEAD - VARIABLE GAIN WITH CLIPPING - INTEGRATABLE INTO TOV OPERATOR HELMET - CONTROL INTERFACE: VOLUME UP/DOWN, SONIC ON/OFF, ULTRA ON/OFF... INFRA ON/OFF, BOOST HI/MED/OFF ----- UGV/TOV ----- ---- AUVS/DAYTON ---- LASER SAFETY IMPLICATIONS IMPLICATIONS FOR DESIGN: - POWER UP SEQUENCE - ABORT

Post-main-sequence Evolution of Icy Minor Planets. II. Water Retention and White Dwarf Pollution around Massive Progenitor Stars

DOE Office of Scientific and Technical Information (OSTI.GOV)

Malamud, Uri; Perets, Hagai B., E-mail: uri.mal@tx.technion.ac.il, E-mail: hperets@physics.technion.ac.il

Most studies suggest that the pollution of white dwarf (WD) atmospheres arises from the accretion of minor planets, but the exact properties of polluting material, and in particular the evidence for water in some cases, are not yet understood. Here we study the water retention of small icy bodies in exo-solar planetary systems, as their respective host stars evolve through and off the main sequence and eventually become WDs. We explore, for the first time, a wide range of star masses and metallicities. We find that the mass of the WD progenitor star is of crucial importance for the retentionmore » of water, while its metallicity is relatively unimportant. We predict that minor planets around lower-mass WD progenitors would generally retain more water and would do so at closer distances from the WD than compared with high-mass progenitors. The dependence of water retention on progenitor mass and other parameters has direct implications for the origin of observed WD pollution, and we discuss how our results and predictions might be tested in the future as more observations of WDs with long cooling ages become available.« less

Needed Now: The 85% Quick Fix in Bio-Defense

DTIC Science & Technology

2004-09-01

That was about ten, twelve, fifteen years ago. Recently, Russian scientists have proclaimed success in developing a Bacillus anthracis strain...commanders to direct personnel to avoid exposure, for example by moving in-doors and turning off heating, ventilation, and air conditioning ( HVAC ...likely be clear of contamination while the air inside buildings may be contaminated because of HVAC operations.20 Therefore, if the HVAC was shut off

The Long War Against Piracy: Historical Trends (Occasional Paper, Number 32)

DTIC Science & Technology

2010-05-01

develop bases closer to their targets.8 Tortuga , located off the northwest coast of Hispaniola, was the first great pirate sanctuary. It maintained...the boucaniers fled to Tortuga , a small island off the northwest coast of Hispaniola, and turned to piracy. The French boucaniers, whose name was...buccaneers commanded large sailing vessels and operated with impunity throughout the Caribbean. Although the Spanish raided Tortuga from time to time

NOTE: Fluoroscopic gating without implanted fiducial markers for lung cancer radiotherapy based on support vector machines

NASA Astrophysics Data System (ADS)

Cui, Ying; Dy, Jennifer G.; Alexander, Brian; Jiang, Steve B.

2008-08-01

Various problems with the current state-of-the-art techniques for gated radiotherapy have prevented this new treatment modality from being widely implemented in clinical routine. These problems are caused mainly by applying various external respiratory surrogates. There might be large uncertainties in deriving the tumor position from external respiratory surrogates. While tracking implanted fiducial markers has sufficient accuracy, this procedure may not be widely accepted due to the risk of pneumothorax. Previously, we have developed a technique to generate gating signals from fluoroscopic images without implanted fiducial markers using template matching methods (Berbeco et al 2005 Phys. Med. Biol. 50 4481-90, Cui et al 2007b Phys. Med. Biol. 52 741-55). In this note, our main contribution is to provide a totally different new view of the gating problem by recasting it as a classification problem. Then, we solve this classification problem by a well-studied powerful classification method called a support vector machine (SVM). Note that the goal of an automated gating tool is to decide when to turn the beam ON or OFF. We treat ON and OFF as the two classes in our classification problem. We create our labeled training data during the patient setup session by utilizing the reference gating signal, manually determined by a radiation oncologist. We then pre-process these labeled training images and build our SVM prediction model. During treatment delivery, fluoroscopic images are continuously acquired, pre-processed and sent as an input to the SVM. Finally, our SVM model will output the predicted labels as gating signals. We test the proposed technique on five sequences of fluoroscopic images from five lung cancer patients against the reference gating signal as ground truth. We compare the performance of the SVM to our previous template matching method (Cui et al 2007b Phys. Med. Biol. 52 741-55). We find that the SVM is slightly more accurate on average (1-3%) than the template matching method, when delivering the target dose. And the average duty cycle is 4-6% longer. Given the very limited patient dataset, we cannot conclude that the SVM is more accurate and efficient than the template matching method. However, our preliminary results show that the SVM is a potentially precise and efficient algorithm for generating gating signals for radiotherapy. This work demonstrates that the gating problem can be considered as a classification problem and solved accordingly.

The CV period minimum

NASA Astrophysics Data System (ADS)

Kolb, Ulrich; Baraffe, Isabelle

Using improved, up-to-date stellar input physics tested against observations of low-mass stars and brown dwarfs we calculate the secular evolution of low-donor-mass CVs, including those which form with a brown dwarf donor star. Our models confirm the mismatch between the calculated minimum period (plus or minus in ~= 70 min) and the observed short-period cut-off (~= 80 min) in the CV period histogram. Theoretical period distributions synthesized from our model sequences always show an accumulation of systems at the minimum period, a feature absent in the observed distribution. We suggest that non-magnetic CVs become unobservable as they are effectively trapped in permanent quiescence before they reach plus or minus in, and that small-number statistics may hide the period spike for magnetic CVs. We calculate the minimum period for high mass transfer rate sequences and discuss the relevance of these for explaining the location of CV secondaries in the orbital-period-spectral-type diagram. We also show that a recently suggested revised mass-radius relation for low-mass main-sequence stars cannot explain the CV period gap.

An analysis of possible off target effects following CAS9/CRISPR targeted deletions of neuropeptide gene enhancers from the mouse genome.

PubMed

Hay, Elizabeth Anne; Khalaf, Abdulla Razak; Marini, Pietro; Brown, Andrew; Heath, Karyn; Sheppard, Darrin; MacKenzie, Alasdair

2017-08-01

We have successfully used comparative genomics to identify putative regulatory elements within the human genome that contribute to the tissue specific expression of neuropeptides such as galanin and receptors such as CB1. However, a previous inability to rapidly delete these elements from the mouse genome has prevented optimal assessment of their function in-vivo. This has been solved using CAS9/CRISPR genome editing technology which uses a bacterial endonuclease called CAS9 that, in combination with specifically designed guide RNA (gRNA) molecules, cuts specific regions of the mouse genome. However, reports of "off target" effects, whereby the CAS9 endonuclease is able to cut sites other than those targeted, limits the appeal of this technology. We used cytoplasmic microinjection of gRNA and CAS9 mRNA into 1-cell mouse embryos to rapidly generate enhancer knockout mouse lines. The current study describes our analysis of the genomes of these enhancer knockout lines to detect possible off-target effects. Bioinformatic analysis was used to identify the most likely putative off-target sites and to design PCR primers that would amplify these sequences from genomic DNA of founder enhancer deletion mouse lines. Amplified DNA was then sequenced and blasted against the mouse genome sequence to detect off-target effects. Using this approach we were unable to detect any evidence of off-target effects in the genomes of three founder lines using any of the four gRNAs used in the analysis. This study suggests that the problem of off-target effects in transgenic mice have been exaggerated and that CAS9/CRISPR represents a highly effective and accurate method of deleting putative neuropeptide gene enhancer sequences from the mouse genome. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

More complete gene silencing by fewer siRNAs: transparent optimized design and biophysical signature

PubMed Central

Ladunga, Istvan

2007-01-01

Highly accurate knockdown functional analyses based on RNA interference (RNAi) require the possible most complete hydrolysis of the targeted mRNA while avoiding the degradation of untargeted genes (off-target effects). This in turn requires significant improvements to target selection for two reasons. First, the average silencing activity of randomly selected siRNAs is as low as 62%. Second, applying more than five different siRNAs may lead to saturation of the RNA-induced silencing complex (RISC) and to the degradation of untargeted genes. Therefore, selecting a small number of highly active siRNAs is critical for maximizing knockdown and minimizing off-target effects. To satisfy these needs, a publicly available and transparent machine learning tool is presented that ranks all possible siRNAs for each targeted gene. Support vector machines (SVMs) with polynomial kernels and constrained optimization models select and utilize the most predictive effective combinations from 572 sequence, thermodynamic, accessibility and self-hairpin features over 2200 published siRNAs. This tool reaches an accuracy of 92.3% in cross-validation experiments. We fully present the underlying biophysical signature that involves free energy, accessibility and dinucleotide characteristics. We show that while complete silencing is possible at certain structured target sites, accessibility information improves the prediction of the 90% active siRNA target sites. Fast siRNA activity predictions can be performed on our web server at . PMID:17169992

Storage of charge carriers on emitter molecules in organic light-emitting diodes

NASA Astrophysics Data System (ADS)

Weichsel, Caroline; Burtone, Lorenzo; Reineke, Sebastian; Hintschich, Susanne I.; Gather, Malte C.; Leo, Karl; Lüssem, Björn

2012-08-01

Organic light-emitting diodes (OLEDs) using the red phosphorescent emitter iridium(III)bis(2-methyldibenzo[f,h]quinoxaline) (acetylacetonate) [Ir(MDQ)2(acac)] are studied by time-resolved electroluminescence measurements. A transient overshoot after voltage turn-off is found, which is attributed to electron accumulation on Ir(MDQ)2(acac) molecules. The mechanism is verified via impedance spectroscopy and by application of positive and negative off-voltages. We calculate the density of accumulated electrons and find that it scales linearly with the doping concentration of the emitter. Using thin quenching layers, we locate the position of the emission zone during normal OLED operation and after voltage turn-off. In addition, the transient overshoot is also observed in three-color white-emitting OLEDs. By time- and spectrally resolved measurements using a streak camera, we directly attribute the overshoot to electron accumulation on Ir(MDQ)2(acac). We propose that similar processes are present in many state-of-the-art OLEDs and believe that the quantification of charge carrier storage will help to improve the efficiency of OLEDs.

A novel high performance ESD power clamp circuit with a small area

NASA Astrophysics Data System (ADS)

Zhaonian, Yang; Hongxia, Liu; Li, Li; Qingqing, Zhuo

2012-09-01

A MOSFET-based electrostatic discharge (ESD) power clamp circuit with only a 10 ns RC time constant for a 0.18-μm process is proposed. A diode-connected NMOSFET is used to maintain a long delay time and save area. The special structure overcomes other shortcomings in this clamp circuit. Under fast power-up events, the gate voltage of the clamp MOSFET does not rise as quickly as under ESD events, the special structure can keep the clamp MOSFET thoroughly off. Under a falsely triggered event, the special structure can turn off the clamp MOSFET in a short time. The clamp circuit can also reject the power supply noise effectively. Simulation results show that the clamp circuit avoids fast false triggering events such as a 30 ns/1.8 V power-up, maintains a 1.2 μs delay time and a 2.14 μs turn-off time, and reduces to about 70% of the RC time constant. It is believed that the proposed clamp circuit can be widely used in high-speed integrated circuits.

Treating the Problem Prostate

MedlinePlus

... this page please turn JavaScript on. Feature: Prostate Enlargement Treating the Problem Prostate Past Issues / Winter 2017 ... Don't put it off." Read More "Prostate Enlargement" Articles Treating the Problem Prostate / Understanding Prostate Enlargement / ...

When the School "Faucet" Turns Off, the Sociocultural "Sprinklers" Turn On: Observing Funds of Knowledge among First and Second Grade English Learners Who Experienced Summer Gains in Reading

ERIC Educational Resources Information Center

Cannon, Shannon Kay

2010-01-01

Based on a mixed methods research design that included an examination of the impact of summer, case study analyses, and survey findings, English learners appear to have an advantage over their non-English learner peers in resisting summer loss and developing social competence. This study examined the effect of summer vacation on the oral reading…

Base drive circuit

DOEpatents

Lange, A.C.

1995-04-04

An improved base drive circuit having a level shifter for providing bistable input signals to a pair of non-linear delays. The non-linear delays provide gate control to a corresponding pair of field effect transistors through a corresponding pair of buffer components. The non-linear delays provide delayed turn-on for each of the field effect transistors while an associated pair of transistors shunt the non-linear delays during turn-off of the associated field effect transistor. 2 figures.

Switching Characteristics of a 4H-SiC Based Bipolar Junction Transistor to 200 C

NASA Technical Reports Server (NTRS)

Niedra, Janis M.

2006-01-01

Static curves and resistive load switching characteristics of a 600 V, 4 A rated, SiC-based NPN bipolar power transistor (BJT) were observed at selected temperatures from room to 200 C. All testing was done in a pulse mode at low duty cycle (approx.0.1 percent). Turn-on was driven by an adjustable base current pulse and turn-off was accelerated by a negative base voltage pulse of 7 V. These base drive signals were implemented by 850 V, gated power pulsers, having rise-times of roughly 10 ns, or less. Base charge sweep-out with a 7 V negative pulse did not produce the large reverse base current pulse seen in a comparably rated Si-based BJT. This may be due to a very low charge storage time. The decay of the collector current was more linear than its exponential-like rise. Switching observations were done at base drive currents (I(sub B)) up to 400 mA and collector currents (I(sub C)) up to 4 A, using a 100 Omega non-inductive load. At I(sub B) = 400 mA and I(sub C) = 4 A, turn-on times typically varied from 80 to 94 ns, over temperatures from 23 to 200 C. As expected, lowering the base drive greatly extended the turn-on time. Similarly, decreasing the load current to I(sub C) = 1 A with I(sub B) = 400 mA produced turn-on times as short as 34 ns. Over the 23 to 200 C range, with I(sub B) = 400 mA and I(sub C) = 4 A, turn-off times were in the range of 72 to 84 ns with the 7 V sweep-out.

A coordinated sequence of distinct flagellar waveforms enables a sharp flagellar turn mediated by squid sperm pH-taxis.

PubMed

Iida, Tomohiro; Iwata, Yoko; Mohri, Tatsuma; Baba, Shoji A; Hirohashi, Noritaka

2017-10-11

Animal spermatozoa navigate by sensing ambient chemicals to reach the site of fertilization. Generally, such chemicals derive from the female reproductive organs or cells. Exceptionally, squid spermatozoa mutually release and perceive carbon dioxide to form clusters after ejaculation. We previously identified the pH-taxis by which each spermatozoon can execute a sharp turn, but how flagellar dynamics enable this movement remains unknown. Here, we show that initiation of the turn motion requires a swim down a steep proton gradient (a theoretical estimation of ≥0.025 pH/s), crossing a threshold pH value of ~5.5. Time-resolved kinematic analysis revealed that the turn sequence results from the rhythmic exercise of two flagellar motions: a stereotypical flagellar 'bent-cane' shape followed by asymmetric wave propagation, which enables a sharp turn in the realm of low Reynolds numbers. This turning episode is terminated by an 'overshoot' trajectory that differs from either straight-line motility or turning. As with bidirectional pH-taxes in some bacteria, squid spermatozoa also showed repulsion from strong acid conditions with similar flagellar kinematics as in positive pH-taxis. These findings indicate that squid spermatozoa might have a unique reorientation mechanism, which could be dissimilar to that of classical egg-guided sperm chemotaxis in other marine invertebrates.

Very High Reflectivity Supermirrors And Their Applications

NASA Astrophysics Data System (ADS)

Mezei, F.

1989-01-01

Very high reflectivity (some 95 % or better) supermirrors, with cut-off angles up to 2 times the critical angle of Ni coated simple total reflection neutron mirrors, can be produced using well established conventional deposition techniques. This performance makes applications involving multiple reflections and transmission geometries feasible, which in turn allow us to use more sophisticated neutron optical systems in order to optimize performance and minimize the amount a scarce supermirrors required. A key feature of several of these novel systems is the distribution of tasks between the several optical components achieving the desired performance by multiple action. The design and characteristics of a series of novel applications, such as polarizing cavities, collimators and guides, non-polarizing guides, beam compressors, deflectors and splitters (most of them tested or being implemented) are the main subjects of the present paper.

Engineering diverse changes in beta-turn propensities in the N-terminal beta-hairpin of ubiquitin reveals significant effects on stability and kinetics but a robust folding transition state.

PubMed

Simpson, Emma R; Meldrum, Jill K; Searle, Mark S

2006-04-04

Using the N-terminal 17-residue beta-hairpin of ubiquitin as a "host" for mutational studies, we have investigated the influence of the beta-turn sequence on protein stability and folding kinetics by replacing the native G-bulged turn (TLTGK) with more flexible analogues (TG3K and TG5K) and a series of four-residue type I' beta-turn sequences, commonly found in beta-hairpins. Although a statistical analysis of type I' turns demonstrates residue preferences at specific sites, the frequency of occurrence appears to only broadly correlate with experimentally determined protein stabilities. The subsequent engineering of context-dependent non-native tertiary contacts involving turn residues is shown to produce large changes in stability. Relatively few point mutations have been described that probe secondary structure formation in ubiquitin in a manner that is independent of tertiary contacts. To this end, we have used the more rigorous rate-equilibrium free energy relationship (Leffler analysis), rather than the two-point phi value analysis, to show for a family of engineered beta-turn mutants that stability (range of approximately 20 kJ/mol) and folding kinetics (190-fold variation in refolding rate) are linearly correlated (alpha(f) = 0.74 +/- 0.08). The data are consistent with a transition state that is robust with regard to a wide range of statistically favored and disfavored beta-turn mutations and implicate a loosely assembled beta-hairpin as a key template in transition state stabilization with the beta-turn playing a central role.

46 CFR 58.05-10 - Automatic shut-off.

Code of Federal Regulations, 2010 CFR

2010-10-01

... AND RELATED SYSTEMS Main Propulsion Machinery § 58.05-10 Automatic shut-off. Main propulsion machinery... controls must shut down main propulsion machinery in case of a failure, such as failure of the lubricating...

46 CFR 58.05-10 - Automatic shut-off.

Code of Federal Regulations, 2011 CFR

2011-10-01

... AND RELATED SYSTEMS Main Propulsion Machinery § 58.05-10 Automatic shut-off. Main propulsion machinery... controls must shut down main propulsion machinery in case of a failure, such as failure of the lubricating...

46 CFR 58.05-10 - Automatic shut-off.

Code of Federal Regulations, 2014 CFR

2014-10-01

... AND RELATED SYSTEMS Main Propulsion Machinery § 58.05-10 Automatic shut-off. Main propulsion machinery... controls must shut down main propulsion machinery in case of a failure, such as failure of the lubricating...

46 CFR 58.05-10 - Automatic shut-off.

Code of Federal Regulations, 2012 CFR

2012-10-01

... AND RELATED SYSTEMS Main Propulsion Machinery § 58.05-10 Automatic shut-off. Main propulsion machinery... controls must shut down main propulsion machinery in case of a failure, such as failure of the lubricating...

46 CFR 58.05-10 - Automatic shut-off.

Code of Federal Regulations, 2013 CFR

2013-10-01

... AND RELATED SYSTEMS Main Propulsion Machinery § 58.05-10 Automatic shut-off. Main propulsion machinery... controls must shut down main propulsion machinery in case of a failure, such as failure of the lubricating...

PIA05044

NASA Image and Video Library

2004-01-11

This mosaic image taken by the navigation camera on the Mars Exploration Rover Spirit represents an overhead view of the rover as it prepares to roll off the lander and onto the martian surface. The yellow arrow illustrates the direction the rover may take to roll safely off the lander. The rover was originally positioned to roll straight forward off the lander (south side of image). However, an airbag is blocking its path. To take this northeastern route, the rover must back up and perform what is likened to a 3-point turn in a cramped parking lot. http://photojournal.jpl.nasa.gov/catalog/PIA05044

'Bird's Eye' View of Egress

NASA Technical Reports Server (NTRS)

2004-01-01

[figure removed for brevity, see original site]

This mosaic image taken by the navigation camera on the Mars Exploration Rover Spirit represents an overhead view of the rover as it prepares to roll off the lander and onto the martian surface. The yellow arrow illustrates the direction the rover may take to roll safely off the lander. The rover was originally positioned to roll straight forward off the lander (south side of image). However, an airbag is blocking its path. To take this northeastern route, the rover must back up and perform what is likened to a 3-point turn in a cramped parking lot.

Q fever at the turn of the century.

PubMed

Chmielewski, Tomasz; Tylewska-Wierzbanowska, Stanisława

2012-01-01

Q fever is an infectious zoonotic disease characterized by sudden fever, headache, and atypical pneumonia, caused by Coxiella burneti--an obligatory intracellular parasite. Based on phylogenetic analysis of the genes sequences, the classification was changed and C. burnetii species was included to the gamma subgroup of the proteobacteria, Legionellales order and Coxiellaceae family. This analysis showed more than 99% sequence similarity of 16SrRNA gene among the strains isolated in different regions of the world. Q fever is a widespread in the world zoonosis. Its main reservoir in the rural environment are farm animals: cows, sheep, goats, and urban pets such as dogs, cats, rabbits. In acute infection these bacteria are detected in various internal organs such as lungs, liver, spleen, and in excretion in urine, faeces and milk. During childbirth, they occur in large number in the amniotic fluid and placenta. Recently, it has been found that free-living amoeba Acanthamoeba castellani may also be a reservoir of the pathogen. The intra-amoebal location of C. burnetii cells was observed.

Four quadrant control circuit for a brushless three-phase dc motor

NASA Technical Reports Server (NTRS)

Nola, Frank J. (Inventor)

1987-01-01

A control circuit is provided for a brushless three-phase dc motor which affords four quadrant control from a single command. The control circuit probes acceleration of the motor in both clockwise and counterclockwise directions and braking and generation in both clockwise and counterclockwise directions. In addition to turning on individual transistors of the transistor pairs connected to the phase windings of the motor for 120 deg periods while the other transistor of that pair is off, the control circuit also provides, in a future mode of operation, turning the two transistors of each pair on and off alternately at a phase modulation frequency during such a 120 deg period. A feedback signal is derived which is proportional to the motor current and which has a polarity consistent with the command signal, such that negative feedback results.

Dilution cycle control for an absorption refrigeration system

DOEpatents

Reimann, Robert C.

1984-01-01

A dilution cycle control system for an absorption refrigeration system is disclosed. The control system includes a time delay relay for sensing shutdown of the absorption refrigeration system and for generating a control signal only after expiration of a preselected time period measured from the sensed shutdown of the absorption refrigeration system, during which the absorption refrigeration system is not restarted. A dilution cycle for the absorption refrigeration system is initiated in response to generation of a control signal by the time delay relay. This control system is particularly suitable for use with an absorption refrigeration system which is frequently cycled on and off since the time delay provided by the control system prevents needless dilution of the absorption refrigeration system when the system is turned off for only a short period of time and then is turned back on.

Investigation of Effectiveness of a Wing Equipped with a 50-percent-chord Sliding Flap, a 30-percent-chord Slotted Flap, and a 30-percent-chord Slat in Deflecting Propeller Slipstreams Downward for Vertical Take-off

NASA Technical Reports Server (NTRS)

Kuhn, Richard E

1957-01-01

Results are presented of an investigation of the effectiveness of a wing equipped with a 50-percent-chord sliding flap and a 30-percent-chord slotted flap in deflecting a propeller slipstream downward for vertical take-off. Tests were conducted at zero forward speed in a large room and included the effects of flap deflection, proximity to the ground, a leading-edge slat, and end plates. A turning angle of about 70 degrees and a resultant force of about 100 percent of the thrust were achieved near the ground. Out of the ground-effect region, the turning angle was also about 70 degrees but the resultant force was reduced to about 86 percent of the thrust.

Analysis of the dynamic avalanche of carrier stored trench bipolar transistor (CSTBT) during clamped inductive turn-off transient

NASA Astrophysics Data System (ADS)

Xue, Peng; Fu, Guicui

2017-03-01

The dynamic avalanche has a huge impact on the switching robustness of carrier stored trench bipolar transistor (CSTBT). The purpose of this work is to investigate the CSTBT's dynamic avalanche mechanism during clamped inductive turn-off transient. At first, with a Mitsubishi 600 V/150 A CSTBT and a Infineon 600 V/200 A field stop insulated gate bipolar transistor (FS-IGBT) utilized, the clamped inductive turn-off characteristics are obtained by double pulse test. The unclamped inductive switching (UIS) test is also utilized to identify the CSTBT's clamping voltage under dynamic avalanche condition. After the test data analysis, it is found that the CSTBT's dynamic avalanche is abnormal and can be triggered under much looser condition than the conventional buffer layer IGBT. The comparison between the FS-IGBT and CSTBT's experimental results implies that the CSTBT's abnormal dynamic avalanche phenomenon may be induced by the carrier storage (CS) layer. Based on the semiconductor physics, the electric field distribution and dynamic avalanche generation in the depletion region are analyzed. The analysis confirms that the CS layer is the root cause of the CSTBT's abnormal dynamic avalanche mechanism. Moreover, the CSTBT's negative gate capacitance effect is also investigated to clarify the underlying mechanism of the gate voltage bump observed in the test. In the end, the mixed-mode numerical simulation is utilized to reproduce the CSTBT's dynamic avalanche behavior. The simulation results validate the proposed dynamic avalanche mechanisms.

Repair Sequences in Dysarthric Conversational Speech: A Study in Interactional Phonetics

ERIC Educational Resources Information Center

Rutter, Ben

2009-01-01

This paper presents some findings from a case study of repair sequences in conversations between a dysarthric speaker, Chris, and her interactional partners. It adopts the methodology of interactional phonetics, where turn design, sequence organization, and variation in phonetic parameters are analysed in unison. The analysis focused on the use of…

Age-related modifications in steering behaviour: effects of base-of-support constraints at the turn point.

PubMed

Paquette, Maxime R; Fuller, Jason R; Adkin, Allan L; Vallis, Lori Ann

2008-09-01

This study investigated the effects of altering the base of support (BOS) at the turn point on anticipatory locomotor adjustments during voluntary changes in travel direction in healthy young and older adults. Participants were required to walk at their preferred pace along a 3-m straight travel path and continue to walk straight ahead or turn 40 degrees to the left or right for an additional 2-m. The starting foot and occasionally the gait starting point were adjusted so that participants had to execute the turn using a cross-over step with a narrow BOS or a lead-out step with a wide BOS. Spatial and temporal gait variables, magnitudes of angular segmental movement, and timing and sequencing of body segment reorientation were similar despite executing the turn with a narrow or wide BOS. A narrow BOS during turning generated an increased step width in the step prior to the turn for both young and older adults. Age-related changes when turning included reduced step velocity and step length for older compared to young adults. Age-related changes in the timing and sequencing of body segment reorientation prior to the turn point were also observed. A reduction in walking speed and an increase in step width just prior to the turn, combined with a delay in motion of the center of mass suggests that older adults used a more cautious combined foot placement and hip strategy to execute changes in travel direction compared to young adults. The results of this study provide insight into mobility constraints during a common locomotor task in older adults.

Pattern-based integer sample motion search strategies in the context of HEVC

NASA Astrophysics Data System (ADS)

Maier, Georg; Bross, Benjamin; Grois, Dan; Marpe, Detlev; Schwarz, Heiko; Veltkamp, Remco C.; Wiegand, Thomas

2015-09-01

The H.265/MPEG-H High Efficiency Video Coding (HEVC) standard provides a significant increase in coding efficiency compared to its predecessor, the H.264/MPEG-4 Advanced Video Coding (AVC) standard, which however comes at the cost of a high computational burden for a compliant encoder. Motion estimation (ME), which is a part of the inter-picture prediction process, typically consumes a high amount of computational resources, while significantly increasing the coding efficiency. In spite of the fact that both H.265/MPEG-H HEVC and H.264/MPEG-4 AVC standards allow processing motion information on a fractional sample level, the motion search algorithms based on the integer sample level remain to be an integral part of ME. In this paper, a flexible integer sample ME framework is proposed, thereby allowing to trade off significant reduction of ME computation time versus coding efficiency penalty in terms of bit rate overhead. As a result, through extensive experimentation, an integer sample ME algorithm that provides a good trade-off is derived, incorporating a combination and optimization of known predictive, pattern-based and early termination techniques. The proposed ME framework is implemented on a basis of the HEVC Test Model (HM) reference software, further being compared to the state-of-the-art fast search algorithm, which is a native part of HM. It is observed that for high resolution sequences, the integer sample ME process can be speed-up by factors varying from 3.2 to 7.6, resulting in the bit-rate overhead of 1.5% and 0.6% for Random Access (RA) and Low Delay P (LDP) configurations, respectively. In addition, the similar speed-up is observed for sequences with mainly Computer-Generated Imagery (CGI) content while trading off the bit rate overhead of up to 5.2%.

Searching for Criteria in Evaluating the Monofin Swimming Turn from the Perspective of Coaching and Improving Technique

PubMed Central

Rejman, Marek; Borowska, Grażyna

2008-01-01

This study aims to analysise the selected kinematic parameters of the monofin swimming turn. The high complexity of performing turns is hindered by the large surface of the monofin, which disturbs control and sense of the body in water. A lack of objective data available on monofin swimming turns has resulted in field research connected with the specification of parameters needed for the evaluation of the technique. Therefore, turns observed in elite swimmers contain underlying conclusions for objective criteria, ensuring the highest level of coaching and the improving of turns in young swimmers. Six, high level, male swimmers participated in the study. The subject of the analysis was the fastest turn, from one out of three trial turns made after swimming a distance of 25 m. Images of the turns were collected from two cameras located under water in accordance with the procedures of the previous analyses of freestyle turns. The images were digitized and analysed by the SIMI®- Movement Analysis System. The interdependency of the total turn time and the remaining recorded parameters, constituted the basis for analysis of the kinematic parameters of five turn phases. The interdependency was measured using r- Pearson’s correlation coefficients. The novel character of the subject covered in this study, forced interpretation of the results on the basis of turn analyses in freestyle swimming. The results allow for the creation of a diagram outlinig area of search for an effective and efficient monofin swimming turn mechanism. The activities performed from the moment of wall contact until the commencement of stroking seem to be crucial for turn improvement. A strong belief has resulted that, the correct monofin swimming turn, is more than just a simple consequence of the fastest performance of all its components. The most important criteria in evaluating the quality of the monofin swimming turn are: striving for the optimal extension of wall contact time, push-off time and glide time. Key pointsShort time and large surface of the monofin additionally hinders complexity of the turn performance by disturbance in sensing and controlling body in water. Availability of no objective data on monofin swimming turns resulted in research in the field connected with specifying parameters needed for the technique evaluation.Correct turn technique may help to improve swimming race results.The diagram constructed on the basis of the interdependency of the total turn time and the remaining recorded kinematic parameters should establish the areas of searching for mechanism of effective and efficient monofin swimming turn.The most crucial, from the coaching and improving point of view, seem to be activities which take place from the moment of feet wall contact till the first propulsive movements. Therefore, the high quality of the monofin swimming turn technique is not just a simple consequence of the fastest performance of all its component parts.The most important criteria of the quality in the monofin swimming turn technique are: striving for extending in the optimum scope of wall contact time, the time of the push-off phase and the glide time. PMID:24150136

Structure-based capacitance modeling and power loss analysis for the latest high-performance slant field-plate trench MOSFET

NASA Astrophysics Data System (ADS)

Kobayashi, Kenya; Sudo, Masaki; Omura, Ichiro

2018-04-01

Field-plate trench MOSFETs (FP-MOSFETs), with the features of ultralow on-resistance and very low gate–drain charge, are currently the mainstream of high-performance applications and their advancement is continuing as low-voltage silicon power devices. However, owing to their structure, their output capacitance (C oss), which leads to main power loss, remains to be a problem, especially in megahertz switching. In this study, we propose a structure-based capacitance model of FP-MOSFETs for calculating power loss easily under various conditions. Appropriate equations were modeled for C oss curves as three divided components. Output charge (Q oss) and stored energy (E oss) that were calculated using the model corresponded well to technology computer-aided design (TCAD) simulation, and we validated the accuracy of the model quantitatively. In the power loss analysis of FP-MOSFETs, turn-off loss was sufficiently suppressed, however, mainly Q oss loss increased depending on switching frequency. This analysis reveals that Q oss may become a significant issue in next-generation high-efficiency FP-MOSFETs.

33 CFR 334.30 - Gulf of Maine off Pemaquid Point, Maine; naval sonobuoy test area.

Code of Federal Regulations, 2014 CFR

2014-07-01

..., Maine; naval sonobuoy test area. 334.30 Section 334.30 Navigation and Navigable Waters CORPS OF....30 Gulf of Maine off Pemaquid Point, Maine; naval sonobuoy test area. (a) The area. The test area or... enforced by the Commanding Officer, U.S. Naval Air Station, Brunswick, Maine, or such agencies as he may...

33 CFR 334.30 - Gulf of Maine off Pemaquid Point, Maine; naval sonobuoy test area.

Code of Federal Regulations, 2011 CFR

2011-07-01

..., Maine; naval sonobuoy test area. 334.30 Section 334.30 Navigation and Navigable Waters CORPS OF....30 Gulf of Maine off Pemaquid Point, Maine; naval sonobuoy test area. (a) The area. The test area or... enforced by the Commanding Officer, U.S. Naval Air Station, Brunswick, Maine, or such agencies as he may...

33 CFR 334.30 - Gulf of Maine off Pemaquid Point, Maine; naval sonobuoy test area.

Code of Federal Regulations, 2013 CFR

2013-07-01

..., Maine; naval sonobuoy test area. 334.30 Section 334.30 Navigation and Navigable Waters CORPS OF....30 Gulf of Maine off Pemaquid Point, Maine; naval sonobuoy test area. (a) The area. The test area or... enforced by the Commanding Officer, U.S. Naval Air Station, Brunswick, Maine, or such agencies as he may...

33 CFR 334.30 - Gulf of Maine off Pemaquid Point, Maine; naval sonobuoy test area.

Code of Federal Regulations, 2012 CFR

2012-07-01

..., Maine; naval sonobuoy test area. 334.30 Section 334.30 Navigation and Navigable Waters CORPS OF....30 Gulf of Maine off Pemaquid Point, Maine; naval sonobuoy test area. (a) The area. The test area or... enforced by the Commanding Officer, U.S. Naval Air Station, Brunswick, Maine, or such agencies as he may...

33 CFR 334.30 - Gulf of Maine off Pemaquid Point, Maine; naval sonobuoy test area.

Code of Federal Regulations, 2010 CFR

2010-07-01

..., Maine; naval sonobuoy test area. 334.30 Section 334.30 Navigation and Navigable Waters CORPS OF....30 Gulf of Maine off Pemaquid Point, Maine; naval sonobuoy test area. (a) The area. The test area or... enforced by the Commanding Officer, U.S. Naval Air Station, Brunswick, Maine, or such agencies as he may...

A comparison of coding sequence and cytogenetic localization of the myostatin gene in the dog, red fox, arctic fox and Chinese raccoon dog.

PubMed

Grzes, M; Nowacka-Woszuk, J; Szczerbal, I; Czerwinska, J; Gracz, J; Switonski, M

2009-01-01

The gene encoding myostatin (MSTN), due to its crucial function for growth of skeletal muscle mass, is an important candidate for muscularity. In this study we analyzed the nucleotide sequence and FISH localization of this gene in 4 canids, including 3 farm species. The nucleotide sequence of the MSTN coding fragment turned out to be highly conserved, since its identity among the studied species was very high and varied between 99.4 and 99.7%. Only 1, widely spread, silent single nucleotide polymorphism (SNP) was found in exon 1 of the Chinese raccoon dog. The MSTN gene was localized close to the centromere in one-armed chromosomes of the dog (37q11) and bi-armed chromosomes of the red fox (16p11) and arctic fox (10q11), with an exception of the Chinese raccoon dog chromosome (2q14-q21). This chromosome is orthologous to 3 canine chromosomes and thus the MSTN was found more interstitially. Our results are in agreement with the hypothesis that karyotypes of the canids evolved mainly through centric fusion/fission events, while tandem fusions occurred rarely. (c) 2009 S. Karger AG, Basel.

Beta-and gamma-turns in proteins revisited: a new set of amino acid turn-type dependent positional preferences and potentials.

PubMed

Guruprasad, K; Rajkumar, S

2000-06-01

The number of beta-turns in a representative set of 426 protein three-dimensional crystal structures selected from the recent Protein Data Bank has nearly doubled and the number of gamma-turns in a representative set of 320 proteins has increased over seven times since the previous analysis. Beta-turns (7153) and gamma-turns (911) extracted from these proteins were used to derive a revised set of type-dependent amino acid positional preferences and potentials. Compared with previous results, the preference for proline, methionine and tryptophan has increased and the preference for glutamine, valine, glutamic acid and alanine has decreased for beta-turns. Certain new amino acid preferences were observed for both turn types and individual amino acids showed turn-type dependent positional preferences. The rationale for new amino acid preferences are discussed in the light of hydrogen bonds and other interactions involving the turns. Where main-chain hydrogen bonds of the type NH(i + 3) --> CO(i) were not observed for some beta-turns, other main-chain hydrogen bonds or solvent interactions were observed that possibly stabilize such beta-turns. A number of unexpected isolated beta-turns with proline at i + 2 position were also observed. The NH(i + 2) --> CO(i) hydrogen bond was observed for almost all gamma-turns. Nearly 20% classic gamma-turns and 43% inverse gamma-turns are isolated turns.

Association of presence/absence and on/off patterns of Helicobacter pylori oipA gene with peptic ulcer disease and gastric cancer risks: a meta-analysis.

PubMed

Liu, Jingwei; He, Caiyun; Chen, Moye; Wang, Zhenning; Xing, Chengzhong; Yuan, Yuan

2013-11-20

There are increasing studies examining the relationship between the status of H. pylori oipA gene and peptic ulcer disease (PUD) and gastric cancer (GC) but the results turn out to be controversial. We attempted to clarify whether oipA gene status is linked with PUD and/or GC risks. A systematically literature search was performed through four electronic databases. According to the specific inclusion and exclusion criteria, seven articles were ultimately available for the meta-analysis of oipA presence/absence with PUD and GC, and eleven articles were included for the meta-analysis of oipA on/off status with PUD and GC. For the on/off functional status analysis of oipA gene, the "on" status showed significant associations with increased risks of PUD (OR = 3.97, 95% CI: 2.89, 5.45; P < 0.001) and GC (OR = 2.43, 95% CI: 1.45, 4.07; P = 0.001) compared with gastritis and functional dyspepsia controls. Results of the homogeneity test indicated different effects of oipA "on" status on PUD risk between children and adult subgroups and on GC risk between PCR-sequencing and immunoblot subgroups. For the presence/absence analysis of oipA gene, we found null association of the presence of oipA gene with the risks of PUD (OR = 1.93, 95% CI: 0.60, 6.25; P = 0.278) and GC (OR = 2.09, 95% CI: 0.51, 8.66; P = 0.308) compared with gastritis and functional dyspepsia controls. To be concluded, when oipA exists, the functional "on" status of this gene showed association with increased risks for PUD and GC compared with gastritis and FD controls. However, merely investigating the presence/absence of oipA would overlook the importance of its functional on/off status and would not be reliable to predict risks of PUD and GC. Further large-scale and well-designed studies concerning on/off status of oipA are required to confirm our meta-analysis results.

Chromospherically Active Stars in the RAVE Survey. II. Young Dwarfs in the Solar Neighborhood

NASA Astrophysics Data System (ADS)

Žerjal, M.; Zwitter, T.; Matijevič, G.; Grebel, E. K.; Kordopatis, G.; Munari, U.; Seabroke, G.; Steinmetz, M.; Wojno, J.; Bienaymé, O.; Bland-Hawthorn, J.; Conrad, C.; Freeman, K. C.; Gibson, B. K.; Gilmore, G.; Kunder, A.; Navarro, J.; Parker, Q. A.; Reid, W.; Siviero, A.; Watson, F. G.; Wyse, R. F. G.

2017-01-01

A large sample of over 38,000 chromospherically active candidate solar-like stars and cooler dwarfs from the RAVE survey is addressed in this paper. An improved activity identification with respect to the previous study was introduced to build a catalog of field stars in the solar neighborhood with an excess emission flux in the calcium infrared triplet wavelength region. The central result of this work is the calibration of the age-activity relation for main-sequence dwarfs in a range from a few 10 {Myr} up to a few Gyr. It enabled an order of magnitude age estimation of the entire active sample. Almost 15,000 stars are shown to be younger than 1 {Gyr} and ˜2000 younger than 100 {Myr}. The young age of the most active stars is confirmed by their position off the main sequence in the J - K versus {N}{UV}-V diagram showing strong ultraviolet excess, mid-infrared excess in the J - K versus {W}1-{W}2 diagram, and very cool temperatures (J-K> 0.7). They overlap with the reference pre-main-sequence RAVE stars often displaying X-ray emission. The activity level increasing with the color reveals their different nature from the solar-like stars and probably represents an underlying dynamo-generating magnetic fields in cool stars. Of the RAVE objects from DR5, 50% are found in the TGAS catalog and supplemented with accurate parallaxes and proper motions by Gaia. This makes the database of a large number of young stars in a combination with RAVE’s radial velocities directly useful as a tracer of the very recent large-scale star formation history in the solar neighborhood. The data are available online in the Vizier database.

Developmental predictors of inattention-hyperactivity from pregnancy to early childhood.

PubMed

Foulon, Stéphanie; Pingault, Jean-Baptiste; Larroque, Béatrice; Melchior, Maria; Falissard, Bruno; Côté, Sylvana M

2015-01-01

The objective of the study was to characterize the developmental sequence of pre- and postnatal risk factors for inattention-hyperactivity symptoms in preschoolers. Longitudinal data came from a French population based birth cohort study (EDEN; N = 1311 mother-child pairs followed from the pregnancy onwards). Inattention-hyperactivity symptoms were assessed with the Strengths and Difficulties Questionnaire when participating children were 3 years of age. Potential risk factors were classified in four domains (fetal exposures and child somatic characteristics, child temperament, child neurodevelopmental status, psychosocial environment) and four periods (before pregnancy, prenatal/birth, infancy, toddlerhood). Their role as potential moderator or mediator was tested with path analysis to determine the developmental sequence. A low family socioeconomic status before pregnancy was the main environmental risk factor for inattention-hyperactivity symptoms at 3 years, and its effect occurred via two pathways. The first was a risk pathway, where lower SES was associated with higher maternal depression and anxiety during pregnancy; then to higher maternal and child distress and dysregulation in infancy; and in turn to higher levels of inattention-hyperactivity at 3 years. The second was a protective pathway, where higher SES was associated with longer duration of breastfeeding during infancy; then to better child neurodevelopmental status in toddlerhood; and in turn to lower levels of inattention-hyperactivity at 3 years. This study identified psychosocial factors at several developmental periods that represent potential targets for preventing the emergence of inattention-hyperactivity symptoms in early childhood.

Developmental Predictors of Inattention-Hyperactivity from Pregnancy to Early Childhood

PubMed Central

Foulon, Stéphanie; Pingault, Jean-Baptiste; Melchior, Maria; Falissard, Bruno; Côté, Sylvana M.

2015-01-01

Objective The objective of the study was to characterize the developmental sequence of pre- and postnatal risk factors for inattention-hyperactivity symptoms in preschoolers. Materials and Methods Longitudinal data came from a French population based birth cohort study (EDEN; N = 1311 mother-child pairs followed from the pregnancy onwards). Inattention-hyperactivity symptoms were assessed with the Strengths and Difficulties Questionnaire when participating children were 3 years of age. Potential risk factors were classified in four domains (fetal exposures and child somatic characteristics, child temperament, child neurodevelopmental status, psychosocial environment) and four periods (before pregnancy, prenatal/birth, infancy, toddlerhood). Their role as potential moderator or mediator was tested with path analysis to determine the developmental sequence. Results A low family socioeconomic status before pregnancy was the main environmental risk factor for inattention-hyperactivity symptoms at 3 years, and its effect occurred via two pathways. The first was a risk pathway, where lower SES was associated with higher maternal depression and anxiety during pregnancy; then to higher maternal and child distress and dysregulation in infancy; and in turn to higher levels of inattention-hyperactivity at 3 years. The second was a protective pathway, where higher SES was associated with longer duration of breastfeeding during infancy; then to better child neurodevelopmental status in toddlerhood; and in turn to lower levels of inattention-hyperactivity at 3 years. Discussion This study identified psychosocial factors at several developmental periods that represent potential targets for preventing the emergence of inattention-hyperactivity symptoms in early childhood. PMID:25938453

A machine learning approach for predicting CRISPR-Cas9 cleavage efficiencies and patterns underlying its mechanism of action.

PubMed

Abadi, Shiran; Yan, Winston X; Amar, David; Mayrose, Itay

2017-10-01

The adaptation of the CRISPR-Cas9 system as a genome editing technique has generated much excitement in recent years owing to its ability to manipulate targeted genes and genomic regions that are complementary to a programmed single guide RNA (sgRNA). However, the efficacy of a specific sgRNA is not uniquely defined by exact sequence homology to the target site, thus unintended off-targets might additionally be cleaved. Current methods for sgRNA design are mainly concerned with predicting off-targets for a given sgRNA using basic sequence features and employ elementary rules for ranking possible sgRNAs. Here, we introduce CRISTA (CRISPR Target Assessment), a novel algorithm within the machine learning framework that determines the propensity of a genomic site to be cleaved by a given sgRNA. We show that the predictions made with CRISTA are more accurate than other available methodologies. We further demonstrate that the occurrence of bulges is not a rare phenomenon and should be accounted for in the prediction process. Beyond predicting cleavage efficiencies, the learning process provides inferences regarding patterns that underlie the mechanism of action of the CRISPR-Cas9 system. We discover that attributes that describe the spatial structure and rigidity of the entire genomic site as well as those surrounding the PAM region are a major component of the prediction capabilities.

Differences in production, carbon stocks and biodiversity outcomes of land tenure regimes in the Argentine Dry Chaco

NASA Astrophysics Data System (ADS)

Marinaro, Sofía; Grau, H. Ricardo; Gasparri, Néstor Ignacio; Kuemmerle, Tobias; Baumann, Matthias

2017-04-01

Rising global demand for agricultural products results in agricultural expansion and intensification, with substantial environmental trade-offs. The South American Dry Chaco contains some of the fastest expanding agricultural frontiers worldwide, and includes diverse forms of land management, mainly associated with different land tenure regimes; which in turn are segregated along environmental gradients (mostly rainfall). Yet, how these regimes impact the environment and how trade-offs between production and environmental outcomes varies remains poorly understood. Here, we assessed how biodiversity, biomass stocks, and agricultural production, measured in meat-equivalents, differ among land tenure regimes in the Dry Chaco. We calculated a land-use outcome index (LUO) that combines indices comparing actual vs. potential values of ‘preservation of biodiversity’ (PI), ‘standing biomass’ (BI) and ‘meat production’ (MI). We found land-use outcomes to vary substantially among land-tenure regimes. Protected areas showed a biodiversity index of 0.75, similar to that of large and medium-sized farms (0.72 in both farming systems), and higher than in the other tenure regimes. Biomass index was similar among land tenure regimes, whereas we found the highest median meat production index on indigenous lands (MI = 0.35). Land-use outcomes, however, varied more across different environmental conditions than across land tenure regimes. Our results suggest that in the Argentine Dry Chaco, there is no single land tenure regime that better minimizes the trade-offs between production and environmental outcomes. A useful approach to manage these trade-offs would be to develop geographically explicit guidelines for land-use zoning, identifying the land tenure regimes more appropriate for each zone.

GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

PubMed Central

Nguyen, Nhu T.; Liebers, Matthew; Topkar, Ved V.; Thapar, Vishal; Wyvekens, Nicolas; Khayter, Cyd; Iafrate, A. John; Le, Long P.; Aryee, Martin J.; Joung, J. Keith

2014-01-01

CRISPR RNA-guided nucleases (RGNs) are widely used genome-editing reagents, but methods to delineate their genome-wide off-target cleavage activities have been lacking. Here we describe an approach for global detection of DNA double-stranded breaks (DSBs) introduced by RGNs and potentially other nucleases. This method, called Genome-wide Unbiased Identification of DSBs Enabled by Sequencing (GUIDE-Seq), relies on capture of double-stranded oligodeoxynucleotides into breaks Application of GUIDE-Seq to thirteen RGNs in two human cell lines revealed wide variability in RGN off-target activities and unappreciated characteristics of off-target sequences. The majority of identified sites were not detected by existing computational methods or ChIP-Seq. GUIDE-Seq also identified RGN-independent genomic breakpoint ‘hotspots’. Finally, GUIDE-Seq revealed that truncated guide RNAs exhibit substantially reduced RGN-induced off-target DSBs. Our experiments define the most rigorous framework for genome-wide identification of RGN off-target effects to date and provide a method for evaluating the safety of these nucleases prior to clinical use. PMID:25513782

Required coefficient of friction during turning at self-selected slow, normal, and fast walking speeds.

PubMed

Fino, Peter; Lockhart, Thurmon E

2014-04-11

This study investigated the relationship of required coefficient of friction to gait speed, obstacle height, and turning strategy as participants walked around obstacles of various heights. Ten healthy, young adults performed 90° turns around corner pylons of four different heights at their self selected normal, slow, and fast walking speeds using both step and spin turning strategies. Kinetic data was captured using force plates. Results showed peak required coefficient of friction (RCOF) at push off increased with increased speed (slow μ=0.38, normal μ=0.45, and fast μ=0.54). Obstacle height had no effect on RCOF values. The average peak RCOF for fast turning exceeded the OSHA safety guideline for static COF of μ>0.50, suggesting further research is needed into the minimum static COF to prevent slips and falls, especially around corners. Copyright © 2014 Elsevier Ltd. All rights reserved.

Lift-Off Performance in Flexure Pivot Pad and Hybrid Bearings

DTIC Science & Technology

2008-12-01

and Dawson, M. P., 1998, "Experience in the Use of Flexure Pivot Tilt Pad Bearings in Boiler Feed Water Pumps ," Proc. of the 15th International...freely. Test Procedure 1) Turn on the pump to buffer water to the test bearing. 2) Turn on air to the air seal that prevents water flowing... Pump Users Symposium, Turbomachinery Laboratory, College Station, Texas, pp. 77-84. [6] Rodriguez, L., 2004, “Experimental Frequency-Dependent

Staying Alive: Cancer Cells Expressing Mutant KRas Depend on ERH for Survival | Center for Cancer Research

Cancer.gov

The small G-protein KRas acts like a molecular switch, turning on and off pro-growth signaling pathways within cells when appropriate. In a large number of cancers, KRas is permanently turned on by a variety of mutations and drives the constant growth of these tumor cells. KRas itself has proved to be a poor drug target so researchers in the laboratory of Ji Luo, Ph.D., in

Project Apollo Flight Sequence

NASA Image and Video Library

1966-08-01

Lunar Orbiter's "Typical Flight sequence of Events" turned out to be quite typical indeed, as all five spacecraft performed exactly as planned. -- Published in James R. Hansen, Spaceflight Revolution: NASA Langley Research Center From Sputnik to Apollo, (Washington: NASA, 1995), p. 340.

Adult Catheter Care and Infection Prevention Guide

MedlinePlus

... or diluted bleach; and wipe with a clean paper towel. Work at a comfortable pace -- the risk ... soap (including under fingernails). • Dry with a clean paper towel. Turn water off with the back of ...

Irrigation Controllers Specification and Certification

EPA Pesticide Factsheets

WaterSense labeled irrigation controllers, which act like a thermostat for your sprinkler system telling it when to turn on and off, use local weather and landscape conditions to tailor watering schedules to actual conditions on the site.

Spacecraft Images Comet Target Jets

NASA Image and Video Library

2010-11-04

NASA Deep Impact spacecraft High- and Medium-Resolution Imagers HRI and MRI captured multiple jets emanating from comet Hartley 2 turning on and off while the spacecraft is 8 million kilometers 5 million miles away from the comet.

GUIDEseq: a bioconductor package to analyze GUIDE-Seq datasets for CRISPR-Cas nucleases.

PubMed

Zhu, Lihua Julie; Lawrence, Michael; Gupta, Ankit; Pagès, Hervé; Kucukural, Alper; Garber, Manuel; Wolfe, Scot A

2017-05-15

Genome editing technologies developed around the CRISPR-Cas9 nuclease system have facilitated the investigation of a broad range of biological questions. These nucleases also hold tremendous promise for treating a variety of genetic disorders. In the context of their therapeutic application, it is important to identify the spectrum of genomic sequences that are cleaved by a candidate nuclease when programmed with a particular guide RNA, as well as the cleavage efficiency of these sites. Powerful new experimental approaches, such as GUIDE-seq, facilitate the sensitive, unbiased genome-wide detection of nuclease cleavage sites within the genome. Flexible bioinformatics analysis tools for processing GUIDE-seq data are needed. Here, we describe an open source, open development software suite, GUIDEseq, for GUIDE-seq data analysis and annotation as a Bioconductor package in R. The GUIDEseq package provides a flexible platform with more than 60 adjustable parameters for the analysis of datasets associated with custom nuclease applications. These parameters allow data analysis to be tailored to different nuclease platforms with different length and complexity in their guide and PAM recognition sequences or their DNA cleavage position. They also enable users to customize sequence aggregation criteria, and vary peak calling thresholds that can influence the number of potential off-target sites recovered. GUIDEseq also annotates potential off-target sites that overlap with genes based on genome annotation information, as these may be the most important off-target sites for further characterization. In addition, GUIDEseq enables the comparison and visualization of off-target site overlap between different datasets for a rapid comparison of different nuclease configurations or experimental conditions. For each identified off-target, the GUIDEseq package outputs mapped GUIDE-Seq read count as well as cleavage score from a user specified off-target cleavage score prediction algorithm permitting the identification of genomic sequences with unexpected cleavage activity. The GUIDEseq package enables analysis of GUIDE-data from various nuclease platforms for any species with a defined genomic sequence. This software package has been used successfully to analyze several GUIDE-seq datasets. The software, source code and documentation are freely available at http://www.bioconductor.org/packages/release/bioc/html/GUIDEseq.html .

Ligand-Induced Stabilization of a Duplex-like Architecture Is Crucial for the Switching Mechanism of the SAM-III Riboswitch.

PubMed

Suresh, Gorle; Srinivasan, Harini; Nanda, Shivani; Priyakumar, U Deva

2016-06-21

Riboswitches are structured RNA motifs that control gene expression by sensing the concentrations of specific metabolites and make up a promising new class of antibiotic targets. S-Adenosylmethionine (SAM)-III riboswitch, mainly found in lactic acid bacteria, is involved in regulating methionine and SAM biosynthetic pathways. SAM-III riboswitch regulates the gene expression by switching the translation process on and off with respect to the absence and presence of the SAM ligand, respectively. In this study, an attempt is made to understand the key conformational transitions involved in ligand binding using atomistic molecular dynamics (MD) simulations performed in an explicit solvent environment. G26 is found to recognize the SAM ligand by forming hydrogen bonds, whereas the absence of the ligand leads to opening of the binding pocket. Consistent with experimental results, the absence of the SAM ligand weakens the base pairing interactions between the nucleobases that are part of the Shine-Dalgarno (SD) and anti-Shine-Dalgarno (aSD) sequences, which in turn facilitates recognition of the SD sequence by ribosomes. Detailed analysis reveals that a duplex-like structure formed by nucleotides from different parts of the RNA and the adenine base of the ligand is crucial for the stability of the completely folded state in the presence of the ligand. Previous experimental studies have shown that the SAM-III riboswitch exists in equilibrium between the unfolded and partially folded states in the absence of the ligand, which completely folds upon binding of the ligand. Comparison of the results presented here to the available experimental data indicates the structures obtained using the MD simulations resemble the partially folded state. Thus, this study provides a detailed understanding of the fully and partially folded structures of the SAM-III riboswitch in the presence and absence of the ligand, respectively. This study hypothesizes a dual role for the SAM ligand, which facilitates conformational switching between partially and fully folded states by forming a stable duplex-like structure and strengthening the interactions between SD and aSD nucleotides.

Adapted ECC ozonesonde for long-duration flights aboard boundary-layer pressurised balloons

NASA Astrophysics Data System (ADS)

Gheusi, François; Durand, Pierre; Verdier, Nicolas; Dulac, François; Attié, Jean-Luc; Commun, Philippe; Barret, Brice; Basdevant, Claude; Clenet, Antoine; Derrien, Solène; Doerenbecher, Alexis; El Amraoui, Laaziz; Fontaine, Alain; Hache, Emeric; Jambert, Corinne; Jaumouillé, Elodie; Meyerfeld, Yves; Roblou, Laurent; Tocquer, Flore

2016-12-01

Since the 1970s, the French space agency CNES has developed boundary-layer pressurised balloons (BLPBs) with the capability to transport lightweight scientific payloads at isopycnic level and offer a quasi-Lagrangian sampling of the lower atmosphere over very long distances and durations (up to several weeks).

Electrochemical concentration cell (ECC) ozonesondes are widely used under small sounding balloons. However, their autonomy is limited to a few hours owing to power consumption and electrolyte evaporation. An adaptation of the ECC sonde has been developed specifically for long-duration BLPB flights. Compared to conventional ECC sondes, the main feature is the possibility of programming periodic measurement sequences (with possible remote control during the flight). To increase the ozonesonde autonomy, the strategy has been adopted of short measurement sequences (2-3 min) regularly spaced in time (e.g. every 15 min). The rest of the time, the sonde pump is turned off. Results of preliminary ground-based tests are first presented. In particular, the sonde was able to provide correct ozone concentrations against a reference UV-absorption ozone analyser every 15 min for 4 days. Then we illustrate results from 16 BLBP flights launched over the western Mediterranean during three summer field campaigns of the ChArMEx project (http://charmex.lsce.ipsl.fr): TRAQA in 2012, and ADRIMED and SAFMED in 2013. BLPB drifting altitudes were in the range 0.25-3.2 km. The longest flight lasted more than 32 h and covered more than 1000 km. Satisfactory data were obtained when compared to independent ozone measurements close in space and time. The quasi-Lagrangian measurements allowed a first look at ozone diurnal evolution in the marine boundary layer as well as in the lower free troposphere. During some flight segments, there was indication of photochemical ozone production in the marine boundary layer or even in the free troposphere, at rates ranging from 1 to 2 ppbv h -1, which is slower than previously found in the boundary layer over land in the same region.

Low incidence of SNVs and indels in trio genomes of Cas9-mediated multiplex edited sheep.

PubMed

Wang, Xiaolong; Liu, Jing; Niu, Yiyuan; Li, Yan; Zhou, Shiwei; Li, Chao; Ma, Baohua; Kou, Qifang; Petersen, Bjoern; Sonstegard, Tad; Huang, Xingxu; Jiang, Yu; Chen, Yulin

2018-05-25

The simplicity of the CRISPR/Cas9 system has enabled its widespread applications in generating animal models, functional genomic screening and in treating genetic and infectious diseases. However, unintended mutations produced by off-target CRISPR/Cas9 nuclease activity may lead to negative consequences. Especially, a very recent study found that gene editing can introduce hundreds of unintended mutations into the genome, and have attracted wide attention. To address the off-target concerns, urgent characterization of the CRISPR/Cas9-mediated off-target mutagenesis is highly anticipated. Here we took advantage of our previously generated gene-edited sheep and performed family trio-based whole genome sequencing which is capable of discriminating variants in the edited progenies that are inherited, naturally generated, or induced by genetic modification. Three family trios were re-sequenced at a high average depth of genomic coverage (~ 25.8×). After developing a pipeline to comprehensively analyze the sequence data for de novo single nucleotide variants, indels and structural variations from the genome; we only found a single unintended event in the form of a 2.4 kb inversion induced by site-specific double-strand breaks between two sgRNA targeting sites at the MSTN locus with a low incidence. We provide the first report on the fidelity of CRISPR-based modification for sheep genomes targeted simultaneously for gene breaks at three coding sequence locations. The trio-based sequencing approach revealed almost negligible off-target modifications, providing timely evidences of the safe application of genome editing in vivo with CRISPR/Cas9.

Kinematics and Shoulder Belt Position of Child Rear Seat Passengers during Vehicle Maneuvers

PubMed Central

Bohman, Katarina; Stockman, Isabelle; Jakobsson, Lotta; Osvalder, Anna-Lisa; Bostrom, Ola; Arbogast, Kristy B.

2011-01-01

Head impact to the seat back has been identified as one important injury causation scenario for seat belt restrained, head-injured children and previous research highlighted vehicle maneuvers prior to impact as possible contributing factors. The aim was to quantify kinematics of child occupants during swerving maneuvers focusing on the child’s lateral movement and seat belt position relative to the child’s shoulder. A study was conducted on a closed-circuit test track with 16 children aged 4–12, restrained in the rear seat of a modern passenger vehicle. A professional driving instructor drove at 50 km/h making sharp turns in a repeatable fashion, resulting in inboard motion of the children. The children were exposed to two turns in each of two restraint systems. Shorter children were on a booster or highback booster cushion. The taller children were seated on a booster cushion or with only a lap and shoulder seat belt. Four film cameras were fixed in the vehicle monitoring the child. Vehicle data were also collected. The seat belt slipped off the shoulder in 1 of 5 turns, varying by age and restraint type. Among shorter children, the belt slipped off in a majority of turns when seated on a booster cushion while the belt remained on the shoulder when seated on the highback booster cushion. Among taller children, the shoulder belt moved far laterally on the shoulder in half of the turns. This data provides valuable knowledge on possible pre-impact postures of children as a result of vehicle swerving maneuvers for a variety of restraint systems. PMID:22105379

Germanium Lift-Off Masks for Thin Metal Film Patterning

NASA Technical Reports Server (NTRS)

Brown, Ari

2012-01-01

A technique has been developed for patterning thin metallic films that are, in turn, used to fabricate microelectronics circuitry and thin-film sensors. The technique uses germanium thin films as lift-off masks. This requires development of a technique to strip or undercut the germanium chemically without affecting the deposited metal. Unlike in the case of conventional polymeric lift-off masks, the substrate can be exposed to very high temperatures during processing (sputter deposition). The reason why polymeric liftoff masks cannot be exposed to very high temperatures (greater than 100 C) is because (a) they can become cross linked, making lift-off very difficult if not impossible, and (b) they can outgas nitrogen and oxygen, which then can react with the metal being deposited. Consequently, this innovation is expected to find use in the fabrication of transition edge sensors and microwave kinetic inductance detectors, which use thin superconducting films deposited at high temperature as their sensing elements. Transition edge sensors, microwave kinetic inductance detectors, and their circuitry are comprised of superconducting thin films, for example Nb and TiN. Reactive ion etching can be used to pattern these films; however, reactive ion etching also damages the underlying substrate, which is unwanted in many instances. Polymeric lift-off techniques permit thin-film patterning without any substrate damage, but they are difficult to remove and the polymer can outgas during thin-film deposition. The outgassed material can then react with the film with the consequence of altered and non-reproducible materials properties, which, in turn, is deleterious for sensors and their circuitry. The purpose of this innovation was to fabricate a germanium lift-off mask to be used for patterning thin metal films.

COSMIC-LAB: unveling the true nature of Terzan 5, a pristine fragment of the Galactic bulge

NASA Astrophysics Data System (ADS)

Ferraro, Francesco

2012-10-01

We have discovered that Terzan5, a stellar system in the Galactic bulge, harbors two stellar populations with different iron content {Delta[Fe/H] 0.5 dex} and possibly different ages {Ferraro et al. 2009, Nature 462, 483}. Moreover, the observed chemical patterns {Origlia et al. 2011, ApJ 726, L20} significantly differ from those observed in any known genuine GC. These evidences demonstrate that, similarly to omega Centauri in the halo, Terzan5 is NOT a genuine globular cluster {GC}, but a stellar system that was able to retain the gas ejected by violent supernova {SN} explosions.Indeed the striking chemical similarity with the bulge stars suggests that Terzan5 and the Galactic bulge shared the same star formation and chemical enrichment processes, driven by an exceptional amount of SNeII explosions {this is also the key to understand the origin of the extraordinary population of millisecond pulsars in Terzan5}. A quite intriguing scenario is emerging from these observations: Terzan5 could be the relic of one of the massive clumps that contributed {through strong dynamical interactions with other pre-formed and internally-evolved sub-structures} to the formation of the Galactic bulge.Here we propose to use the WFC3 to accurately measure the age of the two populations directly from the main sequence turn-off luminosities. Precisely dating the first and second burst of star formation is a crucial step for the correct reconstruction of the evolutionary history of Terzan5, with a significant impact on our comprehension of the formation processes of the Milky Way bulge and, more in general, of galactic spheroids.

The PAndAS Field of Streams: Stellar Structures in the Milky Way Halo toward Andromeda and Triangulum

NASA Astrophysics Data System (ADS)

Martin, Nicolas F.; Ibata, Rodrigo A.; Rich, R. Michael; Collins, Michelle L. M.; Fardal, Mark A.; Irwin, Michael J.; Lewis, Geraint F.; McConnachie, Alan W.; Babul, Arif; Bate, Nicholas F.; Chapman, Scott C.; Conn, Anthony R.; Crnojević, Denija; Ferguson, Annette M. N.; Mackey, A. Dougal; Navarro, Julio F.; Peñarrubia, Jorge; Tanvir, Nial T.; Valls-Gabaud, David

2014-05-01

We reveal the highly structured nature of the Milky Way (MW) stellar halo within the footprint of the Pan-Andromeda Archaeological Survey (PAndAS) photometric survey from blue main sequence (MS) and MS turn-off stars. We map no fewer than five stellar structures within a heliocentric range of ~5-30 kpc. Some of these are known (the Monoceros Ring, the Pisces/Triangulum globular cluster stream), but we also uncover three well-defined stellar structures that could be, at least partly, responsible for the so-called Triangulum/Andromeda and Triangulum/Andromeda 2 features. In particular, we trace a new faint stellar stream located at a heliocentric distance of ~17 kpc. With a surface brightness of Σ V ~ 32-32.5 mag arcsec-2, it follows an orbit that is almost parallel to the Galactic plane north of M31 and has so far eluded surveys of the MW halo as these tend to steer away from regions dominated by the Galactic disk. Investigating our follow-up spectroscopic observations of PAndAS, we serendipitously uncover a radial velocity signature from stars that have colors and magnitudes compatible with the stream. From the velocity of eight likely member stars, we show that this stellar structure is dynamically cold, with an unresolved velocity dispersion that is lower than 7.1 km s-1 at the 90% confidence level. Along with the width of the stream (300-650 pc), its dynamics point to a dwarf-galaxy-accretion origin. The numerous stellar structures we can map in the MW stellar halo between 5 and 30 kpc and their varying morphology is a testament to the complex nature of the stellar halo at these intermediate distances.

A Unified Picture of Mass Segregation in Globular Clusters

NASA Astrophysics Data System (ADS)

Watkins, Laura

2017-08-01

The sensitivity, stability and longevity of HST have opened up an exciting new parameter space: we now have velocity measurements, in the form of proper motions (PMs), for stars from the tip of the red giant branch to a few magnitudes below the main-sequence turn off for a large sample of globular clusters (GCs). For the very first time, we have the opportunity to measure both kinematic and spatial dependences on stellar mass in GCs.The formation and evolution histories of GCs are poorly understood, so too are their intermediate-mass black hole populations and binary fractions. However, the current structure and dynamical state of a GC is directly determined by its past history and its components, so by understanding the former we can gain insight into the latter. Quantifying variations in spatial structure for stars of different mass is extremely difficult with photometry alone as datasets are inhomogenous and incomplete. We require kinematic data for stars that span a range of stellar masses, combined with proper dynamical modelling. We now have the data in hand, but still lack the models needed to maximise the scientific potential of our HST datasets.Here, we propose to extend existing single-mass discrete dynamical-modelling tools to include kinematic and spatial variations with stellar mass, and verify the upgrades using mock data generated from N-body models. We will then apply the models to HST PM data and directly quantify energy equipartition and mass segregation in the GCs. The theoretical phase of the project is vital for the success of the subsequent data analysis, and will serve as a benchmark for future observational campaigns with HST, JWST and beyond.

The search for multiple populations in Magellanic Cloud clusters - IV. Coeval multiple stellar populations in the young star cluster NGC 1978

NASA Astrophysics Data System (ADS)

Martocchia, S.; Niederhofer, F.; Dalessandro, E.; Bastian, N.; Kacharov, N.; Usher, C.; Cabrera-Ziri, I.; Lardo, C.; Cassisi, S.; Geisler, D.; Hilker, M.; Hollyhead, K.; Kozhurina-Platais, V.; Larsen, S.; Mackey, D.; Mucciarelli, A.; Platais, I.; Salaris, M.

2018-07-01

We have recently shown that the ˜2 Gyr old Large Magellanic Cloud star cluster NGC 1978 hosts multiple populations in terms of star-to-star abundance variations in [N/Fe]. These can be seen as a splitting or spread in the subgiant and red giant branches (SGB and RGB) when certain photometric filter combinations are used. Because of its relative youth, NGC 1978 can be used to place stringent limits on whether multiple bursts of star formation have taken place within the cluster, as predicted by some models for the origin of multiple populations. We carry out two distinct analyses to test whether multiple star formation epochs have occurred within NGC 1978. First, we use ultraviolet colour-magnitude diagrams (CMDs) to select stars from the first and second population along the SGB, and then compare their positions in optical CMDs, where the morphology is dominantly controlled by age as opposed to multiple population effects. We find that the two populations are indistinguishable, with age differences of 1 ± 20 Myr between them. This is in tension with predictions from the asymptotic giant branch scenario for the origin of multiple populations. Second, we estimate the broadness of the main-sequence turn-off (MSTO) of NGC 1978 and we report that it is consistent with the observational errors. We find an upper limit of ˜65 Myr on the age spread in the MSTO of NGC 1978. This finding is in conflict with the age spread scenario as origin of the extended MSTO in intermediate-age clusters, while it fully supports predictions from the stellar rotation model.

The PAndAS field of streams: Stellar structures in the milky way halo toward Andromeda and Triangulum

DOE Office of Scientific and Technical Information (OSTI.GOV)

Martin, Nicolas F.; Ibata, Rodrigo A.; Rich, R. Michael

We reveal the highly structured nature of the Milky Way (MW) stellar halo within the footprint of the Pan-Andromeda Archaeological Survey (PAndAS) photometric survey from blue main sequence (MS) and MS turn-off stars. We map no fewer than five stellar structures within a heliocentric range of ∼5-30 kpc. Some of these are known (the Monoceros Ring, the Pisces/Triangulum globular cluster stream), but we also uncover three well-defined stellar structures that could be, at least partly, responsible for the so-called Triangulum/Andromeda and Triangulum/Andromeda 2 features. In particular, we trace a new faint stellar stream located at a heliocentric distance of ∼17more » kpc. With a surface brightness of Σ {sub V} ∼ 32-32.5 mag arcsec{sup –2}, it follows an orbit that is almost parallel to the Galactic plane north of M31 and has so far eluded surveys of the MW halo as these tend to steer away from regions dominated by the Galactic disk. Investigating our follow-up spectroscopic observations of PAndAS, we serendipitously uncover a radial velocity signature from stars that have colors and magnitudes compatible with the stream. From the velocity of eight likely member stars, we show that this stellar structure is dynamically cold, with an unresolved velocity dispersion that is lower than 7.1 km s{sup –1} at the 90% confidence level. Along with the width of the stream (300-650 pc), its dynamics point to a dwarf-galaxy-accretion origin. The numerous stellar structures we can map in the MW stellar halo between 5 and 30 kpc and their varying morphology is a testament to the complex nature of the stellar halo at these intermediate distances.« less

Stellar Populations and Nearby Galaxies with the LSST

NASA Astrophysics Data System (ADS)

Saha, Abhijit; Olsen, K.; Monet, D. G.; LSST Stellar Populations Collaboration

2009-01-01

The LSST will produce a multi-color map and photometric object catalog of half the sky to r=27.6 (AB mag; 5-sigma). Time-space sampling of each field spanning ten years will allow variability, proper motion and parallax measurements for objects brighter than r=24.7. As part of providing an unprecedented map of the Galaxy, the accurate multi-band photometry will permit photometric parallaxes, chemical abundances and a handle on ages via colors at turn-off for main-sequence (MS) stars at all distances within the Galaxy as well as in the Magellanic Clouds, and dwarf satellites of the Milky Way. This will support comprehensive studies of star formation histories and chemical evolution for field stars. The structures of the Clouds and dwarf spheroidals will be traced with the MS stars, to equivalent surface densities fainter than 35 mag/square arc-second. With geometric parallax accuracy of 1 milli-arc-sec, comparable to HIPPARCOS but reaching more than 10 magnitudes fainter, a robust complete sample of solar neighborhood stars will be obtained. The LSST time sampling will identify and characterize variable stars of all types, from time scales of 1 hr to several years, a feast for variable star astrophysics. The combination of wide coverage, multi-band photometry, time sampling and parallax taken together will address several key problems: e.g. fine tuning the extragalactic distance scale by examining properties of RR Lyraes and Cepheids as a function of parent populations, extending the faint end of the galaxy luminosity function by discovering them using star count density enhancements on degree scales tracing, and indentifying inter-galactic stars through novae and Long Period Variables.

Looking for Photometric Signatures of Fast Rotation in Intermediate-Age Star Clusters in the Magellanic Clouds

NASA Astrophysics Data System (ADS)

Goudfrooij, Paul

2017-08-01

Recently, deep color-magnitude diagrams from HST data revealed that several massive intermediate-age star clusters in the Magellanic Clouds exhibit extended main-sequence turn-offs (eMSTOs). This discovery posed serious questions regarding the mechanisms responsible for the formation of massive globular clusters and their well-known multiple stellar populations. The nature of eMSTOs is a hotly debated topic of study. Several studies argued that the eMSTOs are caused by an age range of up to a few hundred Myr, while other studies indicate that eMSTOs can instead be caused by a coeval population in which the stars span a range of rotation velocities. Formal evidence to (dis-)prove either scenario still remains at large, in part because stellar tracks that incorporate the effects of rotation have so far only been available for masses > 1.7 Msun whereas the stars in the known eMSTOs of intermediate-age star clusters are less massive. In this proposal we aim to look for photometric signatures of fast rotators in eMSTO clusters that have been observed by HST in three passbands including (at least) F336W and F814W. We will study spreads in different stellar colors, testing against those predicted with the aid of von Zeipel's geometric study for a population of rotating stars with a significant spread in their inclination. Importantly, this spread due to the presence of rotation is predicted to occur along well-defined lines in color-color diagrams, in directions that are distinct from those in color-magnitude diagrams and distinct from the spread predicted for the age range scenario.

New constraints on the star formation history of the star cluster NGC 1856

NASA Astrophysics Data System (ADS)

Correnti, Matteo; Goudfrooij, Paul; Puzia, Thomas H.; de Mink, Selma E.

2015-07-01

We use the Wide Field Camera 3 onboard the Hubble Space Telescope to obtain deep, high-resolution photometry of the young (age ˜ 300 Myr) star cluster NGC 1856 in the Large Magellanic Cloud. We compare the observed colour-magnitude diagram (CMD), after having applied a correction for differential reddening, with Monte Carlo simulations of simple stellar populations (SSPs) of various ages. We find that the main-sequence turn-off (MSTO) region is wider than that derived from the simulation of a single SSP. Using constraints based on the distribution of stars in the MSTO region and the Red Clump, we find that the CMD is best reproduced using a combination of two different SSPs with ages separated by 80 Myr (0.30 and 0.38 Gyr, respectively). However, we cannot formally exclude that the width of the MSTO could be due to a range of stellar rotation velocities if the efficiency of rotational mixing is higher than typically assumed. Using a King-model fit to the surface number density profile in conjunction with dynamical evolution models, we determine the evolution of cluster mass and escape velocity from an age of 10 Myr to the present age, taking into account the possible effects of primordial mass segregation. We find that the cluster has an escape velocity Vesc ≃ 17 km s-1 at an age of 10 Myr, and it remains high enough during a period of ≃100 Myr to retain material ejected by slow winds of first-generation stars. Our results are consistent with the presence of an age spread in NGC 1856, in contradiction to the results of Bastian & Silva-Villa.

The LMC geometry and outer stellar populations from early DES data

DOE PAGES

Balbinot, Eduardo; Plazas, A.; Santiago, B. X.; ...

2015-03-20

The Dark Energy Camera has captured a large set of images as part of Science Verification (SV) for the Dark Energy Survey. The SV footprint covers a large portion of the outer Large Magellanic Cloud (LMC), providing photometry 1.5 magnitudes fainter than the main sequence turn-off of the oldest LMC stellar population. We derive geometrical and structural parameters for various stellar populations in the LMC disc. For the distribution of all LMC stars, we find an inclination of i = –38.14°±0.08° (near side in the North) and a position angle for the line of nodes of θ₀ = 129.51°±0.17°. Wemore » find that stars younger than ~4 Gyr are more centrally concentrated than older stars. Fitting a projected exponential disc shows that the scale radius of the old populations is R >4Gyr = 1.41 ± 0.01 kpc, while the younger population has R <4Gyr = 0.72 ± 0.01 kpc. However, the spatial distribution of the younger population deviates significantly from the projected exponential disc model. The distribution of old stars suggests a large truncation radius of R t = 13.5 ± 0.8 kpc. If this truncation is dominated by the tidal field of the Galaxy, we find that the LMC is ≃24 +9 –6 times less massive than the encircled Galactic mass. By measuring the Red Clump peak magnitude and comparing with the best-fit LMC disc model, we find that the LMC disc is warped and thicker in the outer regions north of the LMC centre. As a result, our findings may either be interpreted as a warped and flared disc in the LMC outskirts, or as evidence of a spheroidal halo component.« less

Co-Constructing Turns as Interactional Competence: Collaborative Turn Sequences in L1 and L2 Spanish

ERIC Educational Resources Information Center

Baxter, Robert Patrick

2017-01-01

"Interactional competence" is a theoretical construct that has only recently been tested empirically. Speakers contribute to an interaction with their individual resources; however, interactional competence is co-constructed by all participants. Interactional competence can be observed in spoken interaction as speakers' combined ability…

Circular permutation of a WW domain: Folding still occurs after excising the turn of the folding-nucleating hairpin

PubMed Central

Kier, Brandon L.; Anderson, Jordan M.; Andersen, Niels H.

2014-01-01

A hyperstable Pin1 WW domain has been circularly permuted via excision of the fold-nucleating turn; it still folds to form the native three-strand sheet and hydrophobic core features. Multiprobe folding dynamics studies of the normal and circularly permuted sequences, as well as their constituent hairpin fragments and comparable-length β-strand-loop-β-strand models, indicate 2-state folding for all topologies. N-terminal hairpin formation is the fold nucleating event for the wild-type sequence; the slower folding circular permutant has a more distributed folding transition state. PMID:24350581