Husna; Budi, Sri Wilarso; Mansur, Irdika; Kusmana, Dan Cecep
Arbuscular Mycorrhizal Fungi (AMF) are categorized as fungi which have symbioses with terrestrial plants and are distributed in various habitat types. The objectives of this research were to investigate the diversity of AMF in stands of kayu kuku (Pericopsis mooniana Thw.) in Southeast Sulawesi. Collection of samples of soil and root were conducted in six locations. Isolation of spores used the method of wet sieving and decanting, whereas AMF identification was conducted by observing morphology of AMF spores. Parameters of AMF diversity, namely species richness, diversity index, dominance index, evenness index and colonization were studied using method of infected root length. Research results showed that location differences affected significantly the spore density and parameters of AMF diversity, except colonization of AMF (p < 0.116). Location around the Governor office showed the highest number of spores (208.6 spores/100 g of soil). Soil chemical properties, such as C, N, P and heavy metal contributed towards AMF spore density and diversity. Soil C and N correlated negatively with spore density. In terms of location, Glomeraceae constituted the genera with the largest number of species and possessed wide distribution in all research locations. In general, natural forest has higher AMF diversity index (Shannon-Weiner diversity index-H'), evenness (E) and species richness (S) as compared with location of PT. Vale Indonesia Tbk.
Chang, Tak Kwin; Talei, Amin; Alaghmand, Sina; Ooi, Melanie Po-Leen
Input selection for data-driven rainfall-runoff models is an important task as these models find the relationship between rainfall and runoff by direct mapping of inputs to output. In this study, two different input selection methods were used: cross-correlation analysis (CCA), and a combination of mutual information and cross-correlation analyses (MICCA). Selected inputs were used to develop adaptive network-based fuzzy inference system (ANFIS) in Sungai Kayu Ara basin, Selangor, Malaysia. The study catchment has 10 rainfall stations and one discharge station located at the outlet of the catchment. A total of 24 rainfall-runoff events (10-min interval) from 1996 to 2004 were selected from which 18 events were used for training and the remaining 6 were reserved for validating (testing) the models. The results of ANFIS models then were compared against the ones obtained by conceptual model HEC-HMS. The CCA and MICCA methods selected the rainfall inputs only from 2 (stations 1 and 5) and 3 (stations 1, 3, and 5) rainfall stations, respectively. ANFIS model developed based on MICCA inputs (ANFIS-MICCA) performed slightly better than the one developed based on CCA inputs (ANFIS-CCA). ANFIS-CCA and ANFIS-MICCA were able to perform comparably to HEC-HMS model where rainfall data of all 10 stations had been used; however, in peak estimation, ANFIS-MICCA was the best model. The sensitivity analysis on HEC-HMS was conducted by recalibrating the model by using the same selected rainfall stations for ANFIS. It was concluded that HEC-HMS model performance deteriorates if the number of rainfall stations reduces. In general, ANFIS was found to be a reliable alternative for HEC-HMS in cases whereby not all rainfall stations are functioning. This study showed that the selected stations have received the highest total rain and rainfall intensity (stations 3 and 5). Moreover, the contributing rainfall stations selected by CCA and MICCA were found to be located near the outlet of
Ho, Yen-Him; Gan, Seng-Neon; Tan, Irene K P
The medium-chain-length polyhydroxyalkanoate (PHA(MCL)) produced by Pseudomonas putida PGA1 using saponified palm kernel oil as the carbon source could degrade readily in water taken from Kayu Ara River in Selangor, Malaysia. A weight loss of 71.3% of the PHA film occurred in 86 d. The pH of the river water medium fell from 7.5 (at d 0) to 4.7 (at d 86), and there was a net release of CO2. In sterilized river water, the PHA film also lost weight and the pH of the water fell, but to lesser extents. The C8 monomer of the PHA was completely removed after 6 d of immersion in the river water, while the proportions of the other monomers (C10, C12, and C14) were reversed from that of the undegraded PHA. By contrast, the monomer composition of the PHA immersed in sterilized river water did not change significantly from that of the undegraded PHA. Scanning electron microscopy showed physical signs of degradation on the PHA film immersed in the river water, but the film immersed in sterilized river water was relatively unblemished. The results thus indicate that the PHA(MCL) was degraded in tropical river water by biologic as well as nonbiologic means. A significant finding is that shorter-chain monomers were selectively removed throughout the entire PHA molecule, and this suggests enzymatic action.