The study of frequency-scan photothermal reflectance technique for thermal diffusivity measurement

DOE PAGES

Hua, Zilong; Ban, Heng; Hurley, David H.

2015-05-05

A frequency scan photothermal reflectance technique to measure thermal diffusivity of bulk samples is studied in this manuscript. Similar to general photothermal reflectance methods, an intensity-modulated heating laser and a constant intensity probe laser are used to determine the surface temperature response under sinusoidal heating. The approach involves fixing the distance between the heating and probe laser spots, recording the phase lag of reflected probe laser intensity with respect to the heating laser frequency modulation, and extracting thermal diffusivity using the phase lag – (frequency) 1/2 relation. The experimental validation is performed on three samples (SiO 2, CaF 2 andmore » Ge), which have a wide range of thermal diffusivities. The measured thermal diffusivity values agree closely with literature values. Lastly, compared to the commonly used spatial scan method, the experimental setup and operation of the frequency scan method are simplified, and the uncertainty level is equal to or smaller than that of the spatial scan method.« less

The study of frequency-scan photothermal reflectance technique for thermal diffusivity measurement

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hua, Zilong; Ban, Heng; Hurley, David H.

A frequency scan photothermal reflectance technique to measure thermal diffusivity of bulk samples is studied in this manuscript. Similar to general photothermal reflectance methods, an intensity-modulated heating laser and a constant intensity probe laser are used to determine the surface temperature response under sinusoidal heating. The approach involves fixing the distance between the heating and probe laser spots, recording the phase lag of reflected probe laser intensity with respect to the heating laser frequency modulation, and extracting thermal diffusivity using the phase lag – (frequency) 1/2 relation. The experimental validation is performed on three samples (SiO 2, CaF 2 andmore » Ge), which have a wide range of thermal diffusivities. The measured thermal diffusivity values agree closely with literature values. Lastly, compared to the commonly used spatial scan method, the experimental setup and operation of the frequency scan method are simplified, and the uncertainty level is equal to or smaller than that of the spatial scan method.« less

Determination of the optical properties of semi-infinite turbid media from frequency-domain reflectance close to the source.

PubMed

Kienle, A; Patterson, M S

1997-09-01

We investigate theoretically the errors in determining the reduced scattering and absorption coefficients of semi-infinite turbid media from frequency-domain reflectance measurements made at small distances between the source and the detector(s). The errors are due to the uncertainties in the measurement of the phase, the modulation and the steady-state reflectance as well as to the diffusion approximation which is used as a theoretical model to describe light propagation in tissue. Configurations using one and two detectors are examined for the measurement of the phase and the modulation and for the measurement of the phase and the steady-state reflectance. Three solutions of the diffusion equation are investigated. We show that measurements of the phase and the steady-state reflectance at two different distances are best suited for the determination of the optical properties close to the source. For this arrangement the errors in the absorption coefficient due to typical uncertainties in the measurement are greater than those resulting from the application of the diffusion approximation at a modulation frequency of 200 MHz. A Monte Carlo approach is also examined; this avoids the errors due to the diffusion approximation.

Applicability of a Diffuse Reflectance Infrared Fourier Transform handheld spectrometer to perform in situ analyses on Cultural Heritage materials.

PubMed

Arrizabalaga, Iker; Gómez-Laserna, Olivia; Aramendia, Julene; Arana, Gorka; Madariaga, Juan Manuel

2014-08-14

This work studies the applicability of a Diffuse Reflectance Infrared Fourier Transform handheld device to perform in situ analyses on Cultural Heritage assets. This portable diffuse reflectance spectrometer has been used to characterise and diagnose the conservation state of (a) building materials of the Guevara Palace (15th century, Segura, Basque Country, Spain) and (b) different 19th century wallpapers manufactured by the Santa Isabel factory (Vitoria-Gasteiz, Basque Country, Spain) and by the well known Dufour and Leroy manufacturers (Paris, France), all of them belonging to the Torre de los Varona Castle (Villanañe, Basque Country, Spain). In all cases, in situ measurements were carried out and also a few samples were collected and measured in the laboratory by diffuse reflectance spectroscopy (DRIFT) in order to validate the information obtained by the handheld instrument. In the analyses performed in situ, distortions in the diffuse reflectance spectra can be observed due to the presence of specular reflection, showing the inverted bands caused by the Reststrahlen effect, in particular on those IR bands with the highest absorption coefficients. This paper concludes that the results obtained in situ by a diffuse reflectance handheld device are comparable to those obtained with laboratory diffuse reflectance spectroscopy equipment and proposes a few guidelines to acquire good spectra in the field, minimising the influence caused by the specular reflection. Copyright © 2014 Elsevier B.V. All rights reserved.

Diffuse and specular characteristics of leaf reflectance

NASA Technical Reports Server (NTRS)

Grant, Lois

1987-01-01

In this paper, the evolution of current understanding of the mechanisms of leaf reflectance is reviewed. The use of measurements of polarized reflectance to separate leaf reflectance into diffuse and specular components is discussed. A section on the factors influencing leaf reflectance - leaf structure and physiological disturbances - is included along with discussion on the manner in which these influences are manifested.

Two years comparative studies on biological effects of environmental UV radiation

NASA Astrophysics Data System (ADS)

Grof, P.; Ronto, Gyorgyi; Gaspar, S.; Berces, A.; Szabo, Laszlo D.

1994-07-01

A method has been developed for determination of the biologically effective UV dose based on T7 phage as biosensor. In field experiments clockwork driven telescope has been used for determining doses from direct and global (direct plus diffuse) solar radiation. On fine summer days at mid-latitude this arrangement allowed the following comparisons: measured doses from direct and global radiation obtained at the same time and measuring site reflecting the biological importance of diffuse radiation; direct and global radiation obtained at the same time and measuring site reflecting the biological importance of diffuse radiation; direct and global doses obtained at the same time on different measuring sites (downtown, suburb, outside the town) reflecting the differences caused by air quality; direct and global doses obtained on the same measuring place, in summertime of two different years reflecting the importance of the long-term measurements for estimating the biological risk caused by increased UV-B radiation; measured data and model calculations.

Determination of Structural and Morphological Parameters of Human Bulbar Conjunctiva from Optical Diffuse Reflectance Spectra

NASA Astrophysics Data System (ADS)

Lisenko, S. A.; Firago, V. A.; Kugeiko, M. M.; Kubarko, A. I.

2016-09-01

We have developed a method for on-the-fl y retrieval of the volume concentration of blood vessels, the average diameter of the blood vessels, the blood oxygenation level, and the molar concentrations of chromophores in the bulbar conjunctiva from its diffuse reflectance spectra, measured when the radiation delivery and detection channels are spatially separated. The relationship between the diffuse reflectance spectrum of the conjunctiva and its unknown parameters is described in terms of an analytical model, constructed on the basis of a highly accurate approximation analog of the Monte Carlo method. We have studied the effect of localization of hemoglobin in erythrocytes and localization of erythrocytes in the blood vessels on the power of the retrieval of structural and morphological parameters for the conjunctiva. We developed a device for obtaining video images of the conjunctiva and contactless measurements of its diffuse reflectance spectrum. By comparing simulated diffuse reflectance spectra of the conjunctiva with the experimental measurements, we established a set of chromophores which must be taken into account in the model for reproducing the experimental data within the measurement error. We observed absorption bands for neuroglobin in the experimental spectra, and provided a theoretical basis for the possibility of determining its absolute concentrations in the conjunctiva. We have shown that our method can detect low bilirubin concentrations in blood.

Epithelial cancers and photon migration: Monte Carlo simulations and diffuse reflectance measurements

NASA Astrophysics Data System (ADS)

Tubiana, Jerome; Kass, Alex J.; Newman, Maya Y.; Levitz, David

2015-07-01

Detecting pre-cancer in epithelial tissues such as the cervix is a challenging task in low-resources settings. In an effort to achieve low cost cervical cancer screening and diagnostic method for use in low resource settings, mobile colposcopes that use a smartphone as their engine have been developed. Designing image analysis software suited for this task requires proper modeling of light propagation from the abnormalities inside tissues to the camera of the smartphones. Different simulation methods have been developed in the past, by solving light diffusion equations, or running Monte Carlo simulations. Several algorithms exist for the latter, including MCML and the recently developed MCX. For imaging purpose, the observable parameter of interest is the reflectance profile of a tissue under some specific pattern of illumination and optical setup. Extensions of the MCX algorithm to simulate this observable under these conditions were developed. These extensions were validated against MCML and diffusion theory for the simple case of contact measurements, and reflectance profiles under colposcopy imaging geometry were also simulated. To validate this model, the diffuse reflectance profiles of tissue phantoms were measured with a spectrometer under several illumination and optical settings for various homogeneous tissues phantoms. The measured reflectance profiles showed a non-trivial deviation across the spectrum. Measurements of an added absorber experiment on a series of phantoms showed that absorption of dye scales linearly when fit to both MCX and diffusion models. More work is needed to integrate a pupil into the experiment.

Light distribution modulated diffuse reflectance spectroscopy.

PubMed

Huang, Pin-Yuan; Chien, Chun-Yu; Sheu, Chia-Rong; Chen, Yu-Wen; Tseng, Sheng-Hao

2016-06-01

Typically, a diffuse reflectance spectroscopy (DRS) system employing a continuous wave light source would need to acquire diffuse reflectances measured at multiple source-detector separations for determining the absorption and reduced scattering coefficients of turbid samples. This results in a multi-fiber probe structure and an indefinite probing depth. Here we present a novel DRS method that can utilize a few diffuse reflectances measured at one source-detector separation for recovering the optical properties of samples. The core of innovation is a liquid crystal (LC) cell whose scattering property can be modulated by the bias voltage. By placing the LC cell between the light source and the sample, the spatial distribution of light in the sample can be varied as the scattering property of the LC cell modulated by the bias voltage, and this would induce intensity variation of the collected diffuse reflectance. From a series of Monte Carlo simulations and phantom measurements, we found that this new light distribution modulated DRS (LDM DRS) system was capable of accurately recover the absorption and scattering coefficients of turbid samples and its probing depth only varied by less than 3% over the full bias voltage variation range. Our results suggest that this LDM DRS platform could be developed to various low-cost, efficient, and compact systems for in-vivo superficial tissue investigation.

Light distribution modulated diffuse reflectance spectroscopy

PubMed Central

Huang, Pin-Yuan; Chien, Chun-Yu; Sheu, Chia-Rong; Chen, Yu-Wen; Tseng, Sheng-Hao

2016-01-01

Typically, a diffuse reflectance spectroscopy (DRS) system employing a continuous wave light source would need to acquire diffuse reflectances measured at multiple source-detector separations for determining the absorption and reduced scattering coefficients of turbid samples. This results in a multi-fiber probe structure and an indefinite probing depth. Here we present a novel DRS method that can utilize a few diffuse reflectances measured at one source-detector separation for recovering the optical properties of samples. The core of innovation is a liquid crystal (LC) cell whose scattering property can be modulated by the bias voltage. By placing the LC cell between the light source and the sample, the spatial distribution of light in the sample can be varied as the scattering property of the LC cell modulated by the bias voltage, and this would induce intensity variation of the collected diffuse reflectance. From a series of Monte Carlo simulations and phantom measurements, we found that this new light distribution modulated DRS (LDM DRS) system was capable of accurately recover the absorption and scattering coefficients of turbid samples and its probing depth only varied by less than 3% over the full bias voltage variation range. Our results suggest that this LDM DRS platform could be developed to various low-cost, efficient, and compact systems for in-vivo superficial tissue investigation. PMID:27375931

A method of online quantitative interpretation of diffuse reflection profiles of biological tissues

NASA Astrophysics Data System (ADS)

Lisenko, S. A.; Kugeiko, M. M.

2013-02-01

We have developed a method of combined interpretation of spectral and spatial characteristics of diffuse reflection of biological tissues, which makes it possible to determine biophysical parameters of the tissue with a high accuracy in real time under conditions of their general variability. Using the Monte Carlo method, we have modeled a statistical ensemble of profiles of diffuse reflection coefficients of skin, which corresponds to a wave variation of its biophysical parameters. On its basis, we have estimated the retrieval accuracy of biophysical parameters using the developed method and investigated the stability of the method to errors of optical measurements. We have showed that it is possible to determine online the concentrations of melanin, hemoglobin, bilirubin, oxygen saturation of blood, and structural parameters of skin from measurements of its diffuse reflection in the spectral range 450-800 nm at three distances between the radiation source and detector.

Noninvasive diagnostics of skin microphysical parameters based on spatially resolved diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Lisenko, S. A.; Kugeiko, M. M.

2013-01-01

The ability to determine noninvasively microphysical parameters (MPPs) of skin characteristic of malignant melanoma was demonstrated. The MPPs were the melanin content in dermis, saturation of tissue with blood vessels, and concentration and effective size of tissue scatterers. The proposed method was based on spatially resolved spectral measurements of skin diffuse reflectance and multiple regressions between linearly independent measurement components and skin MPPs. The regressions were established by modeling radiation transfer in skin with a wide variation of its MPPs. Errors in the determination of skin MPPs were estimated using fiber-optic measurements of its diffuse reflectance at wavelengths of commercially available semiconductor diode lasers (578, 625, 660, 760, and 806 nm) at source-detector separations of 0.23-1.38 mm.

Further laboratory study of the diffuse reflectance spectra of frosts occurring on astronomical objects

NASA Technical Reports Server (NTRS)

Glaser, F. M.

1976-01-01

Oligoclase and bloedite, two mined samples, have been investigated, and the diffuse reflectance spectra are presented. These data are for powdered material, 50 microns to 5 microns size mixture, cooled to 160 K. The reflectivity of the oligoclase sample was also measured at room temperature, about 290 K, and the results at these two temperatures do indicate some tentative differences. A frost of ordinary water was prepared and its spectral reflectance is presented. This result compares reasonably well with measurements made by other investigators.

Optical Reflectance Measurements for Commonly Used Reflectors

NASA Astrophysics Data System (ADS)

Janecek, Martin; Moses, William W.

2008-08-01

When simulating light collection in scintillators, modeling the angular distribution of optical light reflectance from surfaces is very important. Since light reflectance is poorly understood, either purely specular or purely diffuse reflectance is generally assumed. In this paper we measure the optical reflectance distribution for eleven commonly used reflectors. A 440 nm, output power stabilized, un-polarized laser is shone onto a reflector at a fixed angle of incidence. The reflected light's angular distribution is measured by an array of silicon photodiodes. The photodiodes are movable to cover 2pi of solid angle. The light-induced current is, through a multiplexer, read out with a digital multimeter. A LabVIEW program controls the motion of the laser and the photodiode array, the multiplexer, and the data collection. The laser can be positioned at any angle with a position accuracy of 10 arc minutes. Each photodiode subtends 6.3deg, and the photodiode array can be positioned at any angle with up to 10 arc minute angular resolution. The dynamic range for the current measurements is 10 5:1. The measured light reflectance distribution was measured to be specular for several ESR films as well as for aluminum foil, mostly diffuse for polytetrafluoroethylene (PTFE) tape and titanium dioxide paint, and neither specular nor diffuse for Lumirrorreg, Melinexreg and Tyvekreg. Instead, a more complicated light distribution was measured for these three materials.

Developments in the realization of diffuse reflectance scales at NPL

NASA Astrophysics Data System (ADS)

Chunnilall, Christopher J.; Clarke, Frank J. J.; Shaw, Michael J.

2005-08-01

The United Kingdom scales for diffuse reflectance are realized using two primary instruments. In the 360 nm to 2.5 μm spectral region the National Reference Reflectometer (NRR) realizes absolute measurement of reflectance and radiance factor by goniometric measurements. Hemispherical reflectance scales are obtained through the spatial integration of these goniometric measurements. In the mid-infrared region (2.5 μm - 55 μm) the hemispherical reflectance scale is realized by the Absolute Hemispherical Reflectometer (AHR). This paper describes some of the uncertainties resulting from errors in aligning the NRR and non-ideality in sample topography, together with its use to carry out measurements in the 1 - 1.6 μm region. The AHR has previously been used with grating spectrometers, and has now been coupled to a Fourier transform spectrometer.

Picosecond dynamics of excited singlet states in organic microcrystals: Diffuse reflectance laser photolysis study

NASA Astrophysics Data System (ADS)

Ikeda, Noriaki; Koshioka, Masanori; Masuhara, Hiroshi; Yoshihara, Keitaro

1988-09-01

Absorption spectra and picosecond dynamics of the singlet exciton states of benzil and p-terphenyl in a microcrystal have been measured for the first time by analyzing the diffuse reflected spectra of the picosecond continuum.

Polarization properties and microfacet-based modelling of white, grey and coloured matte diffuse reflection standards

NASA Astrophysics Data System (ADS)

Quast, T.; Schirmacher, A.; Hauer, K.-O.; Koo, A.

2018-02-01

To elucidate the influence of polarization in diffuse reflectometry, we performed a series of measurements in several bidirectional geometries and determined the Stokes parameters of the diffusely reflected radiation. Different types of matte reflection standards were used, including several common white standards and ceramic colour standards. The dependence of the polarization on the sample type, wavelength and geometry have been studied systematically, the main influence factors have been identified: The effect is largest at large angles of incidence or detection and at wavelengths where the magnitude of the reflectance is small. The results for the colour standards have been modelled using a microfacet-based reflection theory which is derived from the well-known model of Torrance and Sparrow. Although the theory is very simple and only has three free parameters, the agreement with the measured data is very good, all essential features of the data can be reproduced by the model.

Probe pressure effects on human skin diffuse reflectance and fluorescence spectroscopy measurements

PubMed Central

Lim, Liang; Nichols, Brandon; Rajaram, Narasimhan; Tunnell, James W.

2011-01-01

Diffuse reflectance and fluorescence spectroscopy are popular research techniques for noninvasive disease diagnostics. Most systems include an optical fiber probe that transmits and collects optical spectra in contact with the suspected lesion. The purpose of this study is to investigate probe pressure effects on human skin spectroscopic measurements. We conduct an in-vivo experiment on human skin tissue to study the short-term (<2 s) and long-term (>30 s) effects of probe pressure on diffuse reflectance and fluorescence measurements. Short-term light probe pressure (P0 < 9 mN∕mm2) effects are within 0 ± 10% on all physiological properties extracted from diffuse reflectance and fluorescence measurements, and less than 0 ± 5% for diagnostically significant physiological properties. Absorption decreases with site-specific variations due to blood being compressed out of the sampled volume. Reduced scattering coefficient variation is site specific. Intrinsic fluorescence shows a large standard error, although no specific pressure-related trend is observed. Differences in tissue structure and morphology contribute to site-specific probe pressure effects. Therefore, the effects of pressure can be minimized when the pressure is small and applied for a short amount of time; however, long-term and large pressures induce significant distortions in measured spectra. PMID:21280899

Tissue differentiation by diffuse reflectance spectroscopy for automated oral and maxillofacial laser surgery: ex vivo pilot study

NASA Astrophysics Data System (ADS)

Zam, Azhar; Stelzle, Florian; Tangermann-Gerk, Katja; Adler, Werner; Nkenke, Emeka; Schmidt, Michael; Douplik, Alexandre

2010-02-01

Remote laser surgery lacks of haptic feedback during the laser ablation of tissue. Hence, there is a risk of iatrogenic damage or destruction of anatomical structures like nerves or salivary glands. Diffuse reflectance spectroscopy provides a straightforward and simple approach for optical tissue differentiation. We measured diffuse reflectance from seven various tissue types ex vivo. We applied Linear Discriminant Analysis (LDA) to differentiate the seven tissue types and computed the area under the ROC curve (AUC). Special emphasis was taken on the identification of nerves and salivary glands as the most crucial tissue for maxillofacial surgery. The results show a promise for differentiating tissues as guidance for oral and maxillofacial laser surgery by means of diffuse reflectance.

Interface morphology of Mo/Si multilayer systems with varying Mo layer thickness studied by EUV diffuse scattering.

PubMed

Haase, Anton; Soltwisch, Victor; Braun, Stefan; Laubis, Christian; Scholze, Frank

2017-06-26

We investigate the influence of the Mo-layer thickness on the EUV reflectance of Mo/Si mirrors with a set of unpolished and interface-polished Mo/Si/C multilayer mirrors. The Mo-layer thickness is varied in the range from 1.7 nm to 3.05 nm. We use a novel combination of specular and diffuse intensity measurements to determine the interface roughness throughout the multilayer stack and do not rely on scanning probe measurements at the surface only. The combination of EUV and X-ray reflectivity measurements and near-normal incidence EUV diffuse scattering allows to reconstruct the Mo layer thicknesses and to determine the interface roughness power spectral density. The data analysis is conducted by applying a matrix method for the specular reflection and the distorted-wave Born approximation for diffuse scattering. We introduce the Markov-chain Monte Carlo method into the field in order to determine the respective confidence intervals for all reconstructed parameters. We unambiguously detect a threshold thickness for Mo in both sample sets where the specular reflectance goes through a local minimum correlated with a distinct increase in diffuse scatter. We attribute that to the known appearance of an amorphous-to-crystallization transition at a certain thickness threshold which is altered in our sample system by the polishing.

Parameterization and sensitivity analyses of a radiative transfer model for remote sensing plant canopies

NASA Astrophysics Data System (ADS)

Hall, Carlton Raden

A major objective of remote sensing is determination of biochemical and biophysical characteristics of plant canopies utilizing high spectral resolution sensors. Canopy reflectance signatures are dependent on absorption and scattering processes of the leaf, canopy properties, and the ground beneath the canopy. This research investigates, through field and laboratory data collection, and computer model parameterization and simulations, the relationships between leaf optical properties, canopy biophysical features, and the nadir viewed above-canopy reflectance signature. Emphasis is placed on parameterization and application of an existing irradiance radiative transfer model developed for aquatic systems. Data and model analyses provide knowledge on the relative importance of leaves and canopy biophysical features in estimating the diffuse absorption a(lambda,m-1), diffuse backscatter b(lambda,m-1), beam attenuation alpha(lambda,m-1), and beam to diffuse conversion c(lambda,m-1 ) coefficients of the two-flow irradiance model. Data sets include field and laboratory measurements from three plant species, live oak (Quercus virginiana), Brazilian pepper (Schinus terebinthifolius) and grapefruit (Citrus paradisi) sampled on Cape Canaveral Air Force Station and Kennedy Space Center Florida in March and April of 1997. Features measured were depth h (m), projected foliage coverage PFC, leaf area index LAI, and zenith leaf angle. Optical measurements, collected with a Spectron SE 590 high sensitivity narrow bandwidth spectrograph, included above canopy reflectance, internal canopy transmittance and reflectance and bottom reflectance. Leaf samples were returned to laboratory where optical and physical and chemical measurements of leaf thickness, leaf area, leaf moisture and pigment content were made. A new term, the leaf volume correction index LVCI was developed and demonstrated in support of model coefficient parameterization. The LVCI is based on angle adjusted leaf thickness Ltadj, LAI, and h (m). Its function is to translate leaf level estimates of diffuse absorption and backscatter to the canopy scale allowing the leaf optical properties to directly influence above canopy estimates of reflectance. The model was successfully modified and parameterized to operate in a canopy scale and a leaf scale mode. Canopy scale model simulations produced the best results. Simulations based on leaf derived coefficients produced calculated above canopy reflectance errors of 15% to 18%. A comprehensive sensitivity analyses indicated the most important parameters were beam to diffuse conversion c(lambda, m-1), diffuse absorption a(lambda, m-1), diffuse backscatter b(lambda, m-1), h (m), Q, and direct and diffuse irradiance. Sources of error include the estimation procedure for the direct beam to diffuse conversion and attenuation coefficients and other field and laboratory measurement and analysis errors. Applications of the model include creation of synthetic reflectance data sets for remote sensing algorithm development, simulations of stress and drought on vegetation reflectance signatures, and the potential to estimate leaf moisture and chemical status.

Diffuse reflectance spectroscopy of pre- and post-treated oral submucous fibrosis: an in vivo study

NASA Astrophysics Data System (ADS)

Sivabalan, S.; Ponranjini Vedeswari, C.; Jayachandran, S.; Koteeswaran, D.; Pravda, C.; Aruna, P.; Ganesan, S.

2010-02-01

Oral submucous fibrosis (OSF) is a high risk precancerous condition characterized by changes in the connective tissue fibers of the lamina propria and deeper parts leading to stiffness of the mucosa and restricted mouth opening, fibrosis of the lining mucosa of the upper digestive tract involving the oral cavity, oro- and hypo-pharynx and the upper two-thirds of the oesophagus. Optical reflectance measurements have been used to extract diagnostic information from a variety of tissue types, in vivo. We apply diffuse reflectance spectroscopy to quantitatively monitor tumour response to chemotherapy. Twenty patients with submucous fibrosis were diagnosed with diffuse reflectance spectroscopy and treated with the chemotherapy drug, Dexamethasone sodium phosphate and Hyaluronidase injection for seven weeks and after the treatment they were again subjected to the diffuse reflectance spectroscopy. The major observed spectral alterations on pre and post treated submucous fibrosis is an increase in the diffuse reflectance from 450 to 600 nm. Normal mucosa has showed higher reflectance when compared to the pre and post-treated cases. The spectral changes were quantified and correlated to conventional diagnostic results viz., maximum mouth opening, tongue protrusion and burning sensation. The results of this study suggest that the diffuse reflectance spectroscopy may also be considered as complementary optical techniques to monitor oral tissue transformation.

SeaWiFS long-term solar diffuser reflectance and sensor noise analyses.

PubMed

Eplee, Robert E; Patt, Frederick S; Barnes, Robert A; McClain, Charles R

2007-02-10

The NASA Ocean Biology Processing Group's Calibration and Validation (Cal/Val) team has undertaken an analysis of the mission-long Sea-Viewing Wide Field-of-View Sensor (SeaWiFS) solar calibration time series to assess the long-term degradation of the solar diffuser reflectance over 9 years on orbit. The SeaWiFS diffuser is an aluminum plate coated with YB71 paint. The bidirectional reflectance distribution function of the diffuser was not fully characterized before launch, so the Cal/Val team has implemented a regression of the solar incidence angles and the drift in the node of the satellite's orbit against the diffuser time series to correct for solar incidence angle effects. An exponential function with a time constant of 200 days yields the best fit to the diffuser time series. The decrease in diffuser reflectance over the mission is wavelength dependent, ranging from 9% in the blue (412 nm) to 5% in the red and near infrared (670-865 nm). The Cal/Val team has developed a methodology for computing the signal-to-noise ratio (SNR) for SeaWiFS on orbit from the diffuser time series corrected for both the varying solar incidence angles and the diffuser reflectance degradation. A sensor noise model is used to compare on-orbit SNRs computed for radiances reflected from the diffuser with prelaunch SNRs measured at typical radiances specified for the instrument. To within the uncertainties in the measurements, the SNRs for SeaWiFS have not changed over the mission. The on-orbit performance of the SeaWiFS solar diffuser should offer insight into the long-term on-orbit performance of solar diffusers on other instruments, such as the Moderate-Resolution Imaging Spectrometer [currently flying on the Earth Observing System (EOS) Terra and Aqua satellites], the Visible and Infrared Radiometer Suite [scheduled to fly on the NASA National Polar-orbiting Operational Environmental Satellite System (NPOESS) and NPOESS Preparatory Project (NPP) satellites] and the Advanced Baseline Imager [scheduled to fly on the National Oceanic and Atmospheric Administration Geostationary Environmental Operational Satellite Series R (GOES-R) satellites].

SeaWiFS long-term solar diffuser reflectance and sensor noise analyses

DOE Office of Scientific and Technical Information (OSTI.GOV)

Eplee, Robert E. Jr.; Patt, Frederick S.; Barnes, Robert A.

The NASA Ocean Biology Processing Group's Calibration and Validation(Cal/Val) team has undertaken an analysis of the mission-long Sea-Viewing Wide Field-of-View Sensor (SeaWiFS)solar calibration time series to assess the long-term degradation of the solar diffuser reflectance over 9 years on orbit. The SeaWiFS diffuser is an aluminum plate coated with YB71 paint. The bidirectional reflectance distribution function of the diffuser was not fully characterized before launch,so the Cal/Val team has implemented a regression of the solar incidence angles and the drift in the node of the satellite's orbit against the diffuser time series to correct for solar incidence angle effects. Anmore » exponential function with a time constant of 200 days yields the best fit to the diffuser time series.The decrease in diffuser reflectance over the mission is wavelength dependent,ranging from 9% in the blue(412 nm) to 5% in the red and near infrared(670-865 nm). The Cal/Val team has developed a methodology for computing the signal-to-noise ratio (SNR) for SeaWiFS on orbit from the diffuser time series corrected for both the varying solar incidence angles and the diffuser reflectance degradation. A sensor noise model is used to compare on-orbit SNRs computed for radiances reflected from the diffuser with prelaunch SNRs measured at typical radiances specified for the instrument. To within the uncertainties in the measurements, the SNRs for SeaWiFS have not changed over the mission. The on-orbit performance of the SeaWiFS solar diffuser should offer insight into the long-term on-orbit performance of solar diffusers on other instruments, such as the Moderate-Resolution Imaging Spectrometer [currently flying on the Earth Observing System (EOS) Terra and Aqua satellites], the Visible and Infrared Radiometer Suite [scheduled to fly on the NASA National Polar-orbiting Operational Environmental Satellite System (NPOESS) and NPOESS Preparatory Project (NPP) satellites] and the Advanced Baseline Imager [scheduled to fly on the National Oceanic and Atmospheric Administration Geostationary Environmental Operational Satellite Series R (GOES-R) satellites].« less

SeaWiFS long-term solar diffuser reflectance and sensor noise analyses

NASA Astrophysics Data System (ADS)

Eplee, Robert E., Jr.; Patt, Frederick S.; Barnes, Robert A.; McClain, Charles R.

2007-02-01

The NASA Ocean Biology Processing Group's Calibration and Validation (Cal/Val) team has undertaken an analysis of the mission-long Sea-Viewing Wide Field-of-View Sensor (SeaWiFS) solar calibration time series to assess the long-term degradation of the solar diffuser reflectance over 9 years on orbit. The SeaWiFS diffuser is an aluminum plate coated with YB71 paint. The bidirectional reflectance distribution function of the diffuser was not fully characterized before launch, so the Cal/Val team has implemented a regression of the solar incidence angles and the drift in the node of the satellite's orbit against the diffuser time series to correct for solar incidence angle effects. An exponential function with a time constant of 200 days yields the best fit to the diffuser time series. The decrease in diffuser reflectance over the mission is wavelength dependent, ranging from 9% in the blue (412 nm) to 5% in the red and near infrared (670-865 nm). The Cal/Val team has developed a methodology for computing the signal-to-noise ratio (SNR) for SeaWiFS on orbit from the diffuser time series corrected for both the varying solar incidence angles and the diffuser reflectance degradation. A sensor noise model is used to compare on-orbit SNRs computed for radiances reflected from the diffuser with prelaunch SNRs measured at typical radiances specified for the instrument. To within the uncertainties in the measurements, the SNRs for SeaWiFS have not changed over the mission. The on-orbit performance of the SeaWiFS solar diffuser should offer insight into the long-term on-orbit performance of solar diffusers on other instruments, such as the Moderate-Resolution Imaging Spectrometer [currently flying on the Earth Observing System (EOS) Terra and Aqua satellites], the Visible and Infrared Radiometer Suite [scheduled to fly on the NASA National Polar-orbiting Operational Environmental Satellite System (NPOESS) and NPOESS Preparatory Project (NPP) satellites] and the Advanced Baseline Imager [scheduled to fly on the National Oceanic and Atmospheric Administration Geostationary Environmental Operational Satellite Series R (GOES-R) satellites].

Spectrophotometric Method for Differentiation of Human Skin Melanoma. II. Diagnostic Characteristics

NASA Astrophysics Data System (ADS)

Petruk, V. G.; Ivanov, A. P.; Kvaternyuk, S. M.; Barunb, V. V.

2016-05-01

Experimental data on the spectral dependences of the optical diffuse reflection coefficient for skin from different people with melanoma or nevus are presented in the form of the probability density of the diffuse reflection coefficient for the corresponding pigmented lesions. We propose a noninvasive technique for differentiating between malignant and benign tumors, based on measuring the diffuse reflection coefficient for a specific patient and comparing the value obtained with a pre-set threshold. If the experimental result is below the threshold, then it is concluded that the person has melanoma; otherwise, no melanoma is present. As an example, we consider the wavelength 870 nm. We determine the risk of malignant transformation of a nevus (its transition to melanoma) for different measured diffuse reflection coefficients. We have studied the errors in the method, its operating characteristics and probability characteristics as the threshold diffuse reflection coefficient is varied. We find that the diagnostic confidence, sensitivity, specificity, and effectiveness (accuracy) parameters are maximum (>0.82) for a threshold of 0.45-0.47. The operating characteristics for the proposed technique exceed the corresponding parameters for other familiar optical approaches to melanoma diagnosis. Its distinguishing feature is operation at only one wavelength, and consequently implementation of the experimental technique is simplified and made less expensive.

Light polarization measurements - A method to determine the specular and diffuse light-scattering properties of both leaves and plant canopies

NASA Technical Reports Server (NTRS)

Vanderbilt, V. C.; Grant, L.

1984-01-01

The contributions of diffuse and specular reflection to the total canopy reflection of sunlight are determined experimentally for wheat at two stages of development using spectroradiometer measurements obtained at 13 wavelengths in the 480-720-nm range with a polarizing film in maximum and minimum signal-amplitude positions. The data and computation techniques are presented in tables, diagrams, and graphs, and the need to take specular reflection into account in constructing models of light/canopy interaction is stressed.

Characterization of LANDSAT Panels Using the NIST BRDF Scale from 1100 nm to 2500 nm

NASA Technical Reports Server (NTRS)

Markham, Brian; Tsai, Benjamin K.; Allen, David W.; Cooksey, Catherine; Yoon, Howard; Hanssen, Leonard; Zeng, Jinan; Fulton, Linda; Biggar, Stuart; Markham, Brian

2010-01-01

Many earth observing sensors depend on white diffuse reflectance standards to derive scales of radiance traceable to the St Despite the large number of Earth observing sensors that operate in the reflective solar region of the spectrum, there has been no direct method to provide NIST traceable BRDF measurements out to 2500 rim. Recent developments in detector technology have allowed the NIST reflectance measurement facility to expand the operating range to cover the 250 nm to 2500 nm range. The facility has been modified with and additional detector using a cooled extended range indium gallium arsenide (Extended InGaAs) detector. Measurements were made for two PTFE white diffuse reflectance standards over the 1100 nm to 2500 nm region at a 0' incident and 45' observation angle. These two panels will be used to support the OLI calibration activities. An independent means of verification was established using a NIST radiance transfer facility based on spectral irradiance, radiance standards and a diffuse reflectance plaque. An analysis on the results and associated uncertainties will be discussed.

Determination of optical band gap of powder-form nanomaterials with improved accuracy

NASA Astrophysics Data System (ADS)

Ahsan, Ragib; Khan, Md. Ziaur Rahman; Basith, Mohammed Abdul

2017-10-01

Accurate determination of a material's optical band gap lies in the precise measurement of its absorption coefficients, either from its absorbance via the Beer-Lambert law or diffuse reflectance spectrum via the Kubelka-Munk function. Absorption coefficients of powder-form nanomaterials calculated from absorbance spectrum do not match those calculated from diffuse reflectance spectrum, implying the inaccuracy of the traditional optical band gap measurement method for such samples. We have modified the Beer-Lambert law and the Kubelka-Munk function with proper approximations for powder-form nanomaterials. Applying the modified method for powder-form nanomaterial samples, both absorbance and diffuse reflectance spectra yield exactly the same absorption coefficients and therefore accurately determine the optical band gap.

Measurement and Interpretation of Diffuse Scattering in X-Ray Diffraction for Macromolecular Crystallography

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wall, Michael E.

X-ray diffraction from macromolecular crystals includes both sharply peaked Bragg reflections and diffuse intensity between the peaks. The information in Bragg scattering reflects the mean electron density in the unit cells of the crystal. The diffuse scattering arises from correlations in the variations of electron density that may occur from one unit cell to another, and therefore contains information about collective motions in proteins.

Technique for examining biological materials using diffuse reflectance spectroscopy and the kubelka-munk function

DOEpatents

Alfano, Robert R.; Yang, Yuanlong

2003-09-02

Method and apparatus for examining biological materials using diffuse reflectance spectroscopy and the Kubelka-Munk function. In one aspect, the method is used to determine whether a tissue sample is cancerous or not and comprises the steps of (a) measuring the diffuse reflectance from the tissue sample at a first wavelength and at a second wavelength, wherein the first wavelength is a wavelength selected from the group consisting of 255-265 nm and wherein the second wavelength is a wavelength selected from the group consisting of 275-285 nm; (b) using the Kubelka-Munk function to transform the diffuse reflectance measurement obtained at the first and second wavelengths; and (c) comparing a ratio or a difference of the transformed Kubelka-Munk measurements at the first and second wavelengths to appropriate standards determine whether or not the tissue sample is cancerous. One can use the spectral profile of KMF between 250 nm to 300 nm to determine whether or not the tissue sample is cancerous or precancerous. According to the value at the first and second wavelengths determine whether or not the malignant tissue is invasive or mixed invasive and in situ or carcinoma in situ.

Retrieval of optical properties of skin from measurement and modeling the diffuse reflectance

NASA Astrophysics Data System (ADS)

Douven, Lucien F. A.; Lucassen, Gerald W.

2000-06-01

We present results on the retrieval of skin optical properties obtained by fitting of measurements of the diffuse reflectance of human skin. Reflectance spectra are simulated using an analytical model based on the diffusion approximation. This model is implemented in a simplex fit routine. The skin optical model used consists of five layers representing epidermis, capillary blood plexus, dermis, deep blood plexus and hypodermis. The optical properties of each layer are assumed homogeneously distributed. The main optical absorbers included are melanin in epidermis and blood. The experimental setup consists of a HP photospectrometer equipped with a remote fiber head. Total reflectance spectra were measured in the 400 - 820 nm wavelength range on the volar underarm of 19 volunteers under various conditions influencing the blood content and oxygenation degree. Changes in the reflectance spectra were observed. Using the fit routine changes in blood content in the capillary blood plexus and in the deep blood plexus could be quantified. These showed different influences on the total reflectance. The method can be helpful to quantitatively assess changes in skin color appearance such as occurs in the treatment of port wine stains, blanching, skin irritation and tanning.

The angular distribution of diffusely backscattered light

NASA Astrophysics Data System (ADS)

Vera, M. U.; Durian, D. J.

1997-03-01

The diffusion approximation predicts the angular distribution of light diffusely transmitted through an opaque slab to depend only on boundary reflectivity, independent of scattering anisotropy, and this has been verified by experiment(M.U. Vera and D.J. Durian, Phys. Rev. E 53) 3215 (1996). Here, by contrast, we demonstrate that the angular distribution of diffusely backscattered light depends on scattering anisotropy as well as boundary reflectivity. To model this observation scattering anisotropy is added to the diffusion approximation by a discontinuity in the photon concentration at the source point that is proportional to the average cosine of the scattering angle. We compare the resulting predictions with random walk simulations and with measurements of diffusely backscattered intensity versus angle for glass frits and aqueous suspensions of polystyrene spheres held in air or immersed in a water bath. Increasing anisotropy and boundary reflectivity each tend to flatten the predicted distributions, and for different combinations of anisotropy and reflectivity the agreement between data and predictions ranges from qualitatively to quantitatively good.

Diffuse reflectance spectra measured in vivo in human tissues during Photofrin-mediated pleural photodynamic therapy

NASA Astrophysics Data System (ADS)

Finlay, Jarod C.; Zhu, Timothy C.; Dimofte, Andreea; Friedberg, Joseph S.; Hahn, Stephen M.

2006-02-01

Optimal delivery of light in photodynamic therapy (PDT) requires not only optimal placement and power of light sources, but knowledge of the dynamics of light propagation in the tissue being treated and in the surrounding normal tissue, and of their respective accumulations of sensitizer. In an effort to quantify both tissue optical properties and sensitizer distribution, we have measured fluorescence emission and diffuse reflectance spectra at the surface of a variety of tissue types in the thoracic cavities of human patients. The patients studied here were enrolled in Phase II clinical trials of Photofrin-mediated PDT for the treatment of non-small cell lung cancer and cancers with pleural effusion. Patients were given Photofrin at dose of 2 mg per kg body weight 24 hours prior to treatment. Each patient received surgical resection of the affected lung and pleura. Patients received intracavity PDT at 630nm to a dose of 30 J/cm2, as determined by isotropic detectors sutured to the cavity walls. We measured the diffuse reflectance spectra before and after PDT in various positions within the cavity, including tumor, diaphragm, pericardium, skin, and chest wall muscle in 5 patients. The measurements we acquired using a specially designed fiber optic-based probe consisting of one fluorescence excitation fiber, one white light delivery fiber, and 9 detection fibers spaced at distances from 0.36 to 7.8 mm from the source, all of which are imaged via a spectrograph onto a CCD, allowing measurement of radially-resolved diffuse reflectance and fluorescence spectra. The light sources for these two measurements (a 403-nm diode laser and a halogen lamp, respectively) were blocked by computer-controlled shutters, allowing sequential fluorescence, reflectance, and background acquisition. The diffuse reflectance was analyzed to determine the absorption and scattering spectra of the tissue and from these, the concentration and oxygenation of hemoglobin and the local drug uptake. The total hemoglobin concentration in normal tissues varied from 50 to 300 µM, and the oxygen saturation was generally above 60%. One tumor measured exhibited higher hemoglobin concentration and lower saturation.

Diffuse reflectance of TiO 2 pigmented paints: Spectral dependence of the average pathlength parameter and the forward scattering ratio

NASA Astrophysics Data System (ADS)

Vargas, William E.; Amador, Alvaro; Niklasson, Gunnar A.

2006-05-01

Diffuse reflectance spectra of paint coatings with different pigment concentrations, normally illuminated with unpolarized radiation, have been measured. A four-flux radiative transfer approach is used to model the diffuse reflectance of TiO2 (rutile) pigmented coatings through the solar spectral range. The spectral dependence of the average pathlength parameter and of the forward scattering ratio for diffuse radiation, are explicitly incorporated into this four-flux model from two novel approximations. The size distribution of the pigments has been taken into account to obtain the averages of the four-flux parameters: scattering and absorption cross sections, forward scattering ratios for collimated and isotropic diffuse radiation, and coefficients involved in the expansion of the single particle phase function in terms of Legendre polynomials.

Artificial neural networks for retrieving absorption and reduced scattering spectra from frequency-domain diffuse reflectance spectroscopy at short source-detector separation

PubMed Central

Chen, Yu-Wen; Chen, Chien-Chih; Huang, Po-Jung; Tseng, Sheng-Hao

2016-01-01

Diffuse reflectance spectroscopy (DRS) based on the frequency-domain (FD) technique has been employed to investigate the optical properties of deep tissues such as breast and brain using source to detector separation up to 40 mm. Due to the modeling and system limitations, efficient and precise determination of turbid sample optical properties from the FD diffuse reflectance acquired at a source-detector separation (SDS) of around 1 mm has not been demonstrated. In this study, we revealed that at SDS of 1 mm, acquiring FD diffuse reflectance at multiple frequencies is necessary for alleviating the influence of inevitable measurement uncertainty on the optical property recovery accuracy. Furthermore, we developed artificial neural networks (ANNs) trained by Monte Carlo simulation generated databases that were capable of efficiently determining FD reflectance at multiple frequencies. The ANNs could work in conjunction with a least-square optimization algorithm to rapidly (within 1 second), accurately (within 10%) quantify the sample optical properties from FD reflectance measured at SDS of 1 mm. In addition, we demonstrated that incorporating the steady-state apparatus into the FD DRS system with 1 mm SDS would enable obtaining broadband absorption and reduced scattering spectra of turbid samples in the wavelength range from 650 to 1000 nm. PMID:27446671

Spectral and geometrical variation of the bidirectional reflectance distribution function of diffuse reflectance standards.

PubMed

Ferrero, Alejandro; Rabal, Ana María; Campos, Joaquín; Pons, Alicia; Hernanz, María Luisa

2012-12-20

A study on the variation of the spectral bidirectional reflectance distribution function (BRDF) of four diffuse reflectance standards (matte ceramic, BaSO(4), Spectralon, and white Russian opal glass) is accomplished through this work. Spectral BRDF measurements were carried out and, using principal components analysis, its spectral and geometrical variation respect to a reference geometry was assessed from the experimental data. Several descriptors were defined in order to compare the spectral BRDF variation of the four materials.

Study of the oxidation of uranium by external and diffuse reflectance FTIR spectroscopy using remote-sensing and evacuable cell techniques

NASA Astrophysics Data System (ADS)

Powell, G. L.; Dobbins, A.; Cristy, S. S.; Cliff, T. L.; Meyer, H. M., III; Lucania, J.; Milosevic, Milan

1994-01-01

This report describes the application of reflectance FTIR spectroscopy to the measurement of the oxidation rate of uranium by environmental gases near room temperature. It also describes very efficient evacuable cells designed for 75 degree(s) external reflectance with polarized light and for diffuse reflectance using mid-infrared FTIR spectroscopy. These cells, along with functionally similar remote sensing accessories, have been applied to the study of the oxidation of uranium metal in air, oxygen, and water vapor by precisely measuring the 575 cm-1 band of UO2 and other properties of the corrosion film such as absorbed water and reflective losses caused by film degradation related to pitting or nucleation phenomena.

Selective mode excitation in finite size plasma crystals by diffusely reflected laser light

NASA Astrophysics Data System (ADS)

Schablinski, Jan; Block, Dietmar

2015-02-01

The possibility to use diffuse reflections of a laser beam to exert a force on levitating dust particles is studied experimentally. Measurements and theoretical predictions are found to be in good agreement. Further, the method is applied to test the selective excitation of breathing-like modes in finite dust clusters.

Photothermal Radiometry and Diffuse Reflectance Analysis of Thermally Treated Bones

NASA Astrophysics Data System (ADS)

Trujillo, S.; Martínez-Torres, P.; Quintana, P.; Alvarado-Gil, Juan Jose

2010-05-01

Different fields such as archaeology, biomedicine, forensic science, and pathology involve the analysis of burned bones. In this work, the effects of successive thermal treatments on pig long bones, measured by photothermal radiometry and diffuse reflectance are reported. Measurements were complemented by X-ray diffraction and infrared spectroscopy. Samples were thermally treated for 1 h within the range of 25 °C to 350 °C. The thermal diffusivity and reflectance increase in the low-temperature range, reaching a maximum around 125 °C and decaying at higher temperatures. These results are the consequence of complex modifications occurring in the inorganic and organic bone structure. For lower temperatures dehydration, dehydroxilation, and carbonate loss processes are dominant, followed by collagen denaturing and decompositions, which have an influence on the bone microstructure.

Bidirectional reflectance distribution function effects in ladar-based reflection tomography.

PubMed

Jin, Xuemin; Levine, Robert Y

2009-07-20

Light reflection from a surface is described by the bidirectional reflectance distribution function (BRDF). In this paper, BRDF effects in reflection tomography are studied using modeled range-resolved reflection from well-characterized geometrical surfaces. It is demonstrated that BRDF effects can cause a darkening at the interior boundary of the reconstructed surface analogous to the well-known beam hardening artifact in x-ray transmission computed tomography (CT). This artifact arises from reduced reflection at glancing incidence angles to the surface. It is shown that a purely Lambertian surface without shadowed components is perfectly reconstructed from range-resolved measurements. This result is relevant to newly fabricated carbon nanotube materials. Shadowing is shown to cause crossed streak artifacts similar to limited-angle effects in CT reconstruction. In tomographic reconstruction, these effects can overwhelm highly diffuse components in proximity to specularly reflecting elements. Diffuse components can be recovered by specialized processing, such as reducing glints via thresholded measurements.

Soft tissue differentiation by diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Zam, Azhar; Stelzle, Florian; Nkenke, Emeka; Tangermann-Gerk, Katja; Schmidt, Michael; Adler, Werner; Douplik, Alexandre

2009-07-01

Laser surgery gives the possibility to work remotely which leads to high precision, little trauma and high level sterility. However these advantages are coming with the lack of haptic feedback during the laser ablation of tissue. Therefore additional means are required to control tissue-specific ablation during laser surgery supporting the surgeon regardless of experience and skills. Diffuse Reflectance Spectroscopy provides a straightforward and simple approach for optical tissue differentiation. We measured diffuse reflectance from four various tissue types ex vivo. We applied Linear Discriminant Analysis (LDA) to differentiate the four tissue types and computed the area under the ROC curve (AUC). Special emphasis was taken on the identification of nerve as the most crucial tissue for maxillofacial surgery. The results show a promise for differentiating soft tissues as guidance for tissue-specific laser surgery by means of the diffuse reflectance.

Specular, diffuse and polarized imagery of an oat canopy

NASA Technical Reports Server (NTRS)

Vanderbilt, Vern C.; De Venecia, Kurt J.

1988-01-01

Light, polarized by specular reflection, has been found to be an important part of the light scattered by several measured plant canopies. The authors investigate for one canopy the relative importance of specularly reflected sunlight, specularly reflected light from other sources including skylight, and diffusely upwelling light. Polarization images are used to gain increased understanding of the radiation transfer process in a plant canopy. Analysis of the results suggests that properly analyzed polarized remotely sensed data, acquired under specific atmospheric conditions by a specially designed sensor, potentially provide measures of physiological and morphological states of plants in a canopy.

SWIR calibration of Spectralon reflectance factor

NASA Astrophysics Data System (ADS)

Georgiev, Georgi T.; Butler, James J.; Cooksey, Catherine; Ding, Leibo; Thome, Kurtis J.

2011-11-01

Satellite instruments operating in the reflective solar wavelength region require accurate and precise determination of the Bidirectional Reflectance Factor (BRF) of laboratory-based diffusers used in their pre-flight and on-orbit radiometric calibrations. BRF measurements are required throughout the reflected-solar spectrum from the ultraviolet through the shortwave infrared. Spectralon diffusers are commonly used as a reflectance standard for bidirectional and hemispherical geometries. The Diffuser Calibration Laboratory (DCaL) at NASA's Goddard Space Flight Center is a secondary calibration facility with reflectance measurements traceable to those made by the Spectral Tri-function Automated Reference Reflectometer (STARR) facility at the National Institute of Standards and Technology (NIST). For more than two decades, the DCaL has provided numerous NASA projects with BRF data in the ultraviolet (UV), visible (VIS) and the Near InfraRed (NIR) spectral regions. Presented in this paper are measurements of BRF from 1475 nm to 1625 nm obtained using an indium gallium arsenide detector and a tunable coherent light source. The sample was a 50.8 mm (2 in) diameter, 99% white Spectralon target. The BRF results are discussed and compared to empirically generated data from a model based on NIST certified values of 6°directional-hemispherical spectral reflectance factors from 900 nm to 2500 nm. Employing a new NIST capability for measuring bidirectional reflectance using a cooled, extended InGaAs detector, BRF calibration measurements of the same sample were also made using NIST's STARR from 1475 nm to 1625 nm at an incident angle of 0° and at viewing angle of 45°. The total combined uncertainty for BRF in this ShortWave Infrared (SWIR) range is less than 1%. This measurement capability will evolve into a BRF calibration service in SWIR region in support of NASA remote sensing missions.

Effect of sample thickness on the extracted near-infrared bulk optical properties of Bacillus subtilis in liquid culture.

PubMed

Dzhongova, Elitsa; Harwood, Colin R; Thennadil, Suresh N

2011-11-01

In order to determine the bulk optical properties of a Bacillus subtilis culture during growth phase we investigated the effect of sample thickness on measurements taken with different measurement configurations, namely total diffuse reflectance and total diffuse transmittance. The bulk optical properties were extracted by inverting the measurements using the radiative transfer theory. While the relationship between reflectance and biomass changes with sample thickness and the intensity (absorbance) levels vary significantly for both reflectance and transmittance measurements, the extracted optical properties show consistent behavior in terms of both the relationship with biomass and magnitude. This observation indicates the potential of bulk optical properties for building models that could be more easily transferable compared to those built using raw measurements.

Critical comparison of diffuse reflectance spectroscopy and colorimetry as dermatological diagnostic tools for acanthosis nigricans: a chemometric approach.

PubMed

Devpura, Suneetha; Pattamadilok, Bensachee; Syed, Zain U; Vemulapalli, Pranita; Henderson, Marsha; Rehse, Steven J; Hamzavi, Iltefat; Lim, Henry W; Naik, Ratna

2011-06-01

Quantification of skin changes due to acanthosis nigricans (AN), a disorder common among insulin-resistant diabetic and obese individuals, was investigated using two optical techniques: diffuse reflectance spectroscopy (DRS) and colorimetry. Measurements were obtained from AN lesions on the neck and two control sites of eight AN patients. A principal component/discriminant function analysis successfully differentiated between AN lesion and normal skin with 87.7% sensitivity and 94.8% specificity in DRS measurements and 97.2% sensitivity and 96.4% specificity in colorimetry measurements.

Optical device for thermal diffusivity determination in liquids by reflection of a thermal wave

NASA Astrophysics Data System (ADS)

Sánchez-Pérez, C.; De León-Hernández, A.; García-Cadena, C.

2017-08-01

In this work, we present a device for determination of the thermal diffusivity using the oblique reflection of a thermal wave within a solid slab that is in contact with the medium to be characterized. By using the reflection near a critical angle under the assumption that thermal waves obey Snell's law of refraction with the square root of the thermal diffusivities, the unknown thermal diffusivity is obtained by simple formulae. Experimentally, the sensor response is measured using the photothermal beam deflection technique within a slab that results in a compact device with no contact of the laser probing beam with the sample. We describe the theoretical basis and provide experimental results to validate the proposed method. We determine the thermal diffusivity of tridistilled water and glycerin solutions with an error of less than 0.5%.

Contamination and UV ageing of diffuser targets used in satellite inflight and ground reference test site calibrations

NASA Astrophysics Data System (ADS)

Vaskuri, Anna; Greenwell, Claire; Hessey, Isabel; Tompkins, Jordan; Woolliams, Emma

2018-02-01

Diffuser reflectance targets are key components in in-orbit calibrations and for verifying ground reference test sites. In this work, Spectralon, Diffusil, and Heraeus diffusers were exposed to exhaust gases and ultraviolet (UV) radiation in the ambient air conditions and their degradations were monitored by measuring changes in spectral reflectances. Spectralon is a state-of-the-art diffuser made of polytetrafluoroethylene, and Diffusil and Heraeus diffusers are made of fused silica with gas bubbles inside. Based on the contamination tests, Spectralon degrades faster than fused silica diffusers. For the samples exposed to contamination for 20 minutes, the 250 nm - 400 nm total diffuse spectral reflectance of Spectralon degraded 3-5 times more when exposed to petrol-like emission and 16-23 times more when exposed to diesel-like emission, compared with Diffusil. When the reflectance changes of Spectralon were compared with those of Heraeus, Spectralon degraded 3-4 times more when exposed to petrol-like emission for 20 minutes and 5-7 times more when exposed to diesel-like emission for 7.5 minutes. When the samples contaminated were exposed to UV radiation in the ambient air, their reflectance gradually restored back to the original level. In conclusion, fused silica diffusers are more resistant to hydrocarbon contaminants present in ground reference test sites, and thus more stable under UV radiation in the air.

Radiative transfer simulations of the two-dimensional ocean glint reflectance and determination of the sea surface roughness.

PubMed

Lin, Zhenyi; Li, Wei; Gatebe, Charles; Poudyal, Rajesh; Stamnes, Knut

2016-02-20

An optimized discrete-ordinate radiative transfer model (DISORT3) with a pseudo-two-dimensional bidirectional reflectance distribution function (BRDF) is used to simulate and validate ocean glint reflectances at an infrared wavelength (1036 nm) by matching model results with a complete set of BRDF measurements obtained from the NASA cloud absorption radiometer (CAR) deployed on an aircraft. The surface roughness is then obtained through a retrieval algorithm and is used to extend the simulation into the visible spectral range where diffuse reflectance becomes important. In general, the simulated reflectances and surface roughness information are in good agreement with the measurements, and the diffuse reflectance in the visible, ignored in current glint algorithms, is shown to be important. The successful implementation of this new treatment of ocean glint reflectance and surface roughness in DISORT3 will help improve glint correction algorithms in current and future ocean color remote sensing applications.

Radiative Transfer Simulations of the Two-Dimensional Ocean Glint Reflectance and Determination of the Sea Surface Roughness

NASA Technical Reports Server (NTRS)

Lin, Zhenyi; Li, Wei; Gatebe, Charles; Poudyal, Rajesh; Stamnes, Knut

2016-01-01

An optimized discrete-ordinate radiative transfer model (DISORT3) with a pseudo-two-dimensional bidirectional reflectance distribution function (BRDF) is used to simulate and validate ocean glint reflectances at an infrared wavelength (1036 nm) by matching model results with a complete set of BRDF measurements obtained from the NASA cloud absorption radiometer (CAR) deployed on an aircraft. The surface roughness is then obtained through a retrieval algorithm and is used to extend the simulation into the visible spectral range where diffuse reflectance becomes important. In general, the simulated reflectances and surface roughness information are in good agreement with the measurements, and the diffuse reflectance in the visible, ignored in current glint algorithms, is shown to be important. The successful implementation of this new treatment of ocean glint reflectance and surface roughness in DISORT3 will help improve glint correction algorithms in current and future ocean color remote sensing applications.

Classification of road sign type using mobile stereo vision

NASA Astrophysics Data System (ADS)

McLoughlin, Simon D.; Deegan, Catherine; Fitzgerald, Conor; Markham, Charles

2005-06-01

This paper presents a portable mobile stereo vision system designed for the assessment of road signage and delineation (lines and reflective pavement markers or "cat's eyes"). This novel system allows both geometric and photometric measurements to be made on objects in a scene. Global Positioning System technology provides important location data for any measurements made. Using the system it has been shown that road signs can be classfied by nature of their reflectivity. This is achieved by examining the changes in the reflected light intensity with changes in range (facilitated by stereo vision). Signs assessed include those made from retro-reflective materials, those made from diffuse reflective materials and those made from diffuse reflective matrials with local illumination. Field-testing results demonstrate the systems ability to classify objects in the scene based on their reflective properties. The paper includes a discussion of a physical model that supports the experimental data.

Preliminary results of BTDF calibration of transmissive solar diffusers for remote sensing

NASA Astrophysics Data System (ADS)

Georgiev, Georgi T.; Butler, James J.; Thome, Kurt; Cooksey, Catherine; Ding, Leibo

2016-09-01

Satellite instruments operating in the reflected solar wavelength region require accurate and precise determination of the optical properties of their diffusers used in pre-flight and post-flight calibrations. The majority of recent and current space instruments use reflective diffusers. As a result, numerous Bidirectional Reflectance Distribution Function (BRDF) calibration comparisons have been conducted between the National Institute of Standards and Technology (NIST) and other industry and university-based metrology laboratories. However, based on literature searches and communications with NIST and other laboratories, no Bidirectional Transmittance Distribution Function (BTDF) measurement comparisons have been conducted between National Measurement Laboratories (NMLs) and other metrology laboratories. On the other hand, there is a growing interest in the use of transmissive diffusers in the calibration of satellite, air-borne, and ground-based remote sensing instruments. Current remote sensing instruments employing transmissive diffusers include the Ozone Mapping and Profiler Suite instrument (OMPS) Limb instrument on the Suomi-National Polar-orbiting Partnership (S-NPP) platform,, the Geostationary Ocean Color Imager (GOCI) on the Korea Aerospace Research Institute's (KARI) Communication, Ocean, and Meteorological Satellite (COMS), the Ozone Monitoring Instrument (OMI) on NASA's Earth Observing System (EOS) Aura platform, the Tropospheric Emissions: Monitoring of Pollution (TEMPO) instrument and the Geostationary Environmental Monitoring Spectrometer (GEMS).. This ensemble of instruments requires validated BTDF measurements of their onboard transmissive diffusers from the ultraviolet through the near infrared. This paper presents the preliminary results of a BTDF comparison between the NASA Diffuser Calibration Laboratory (DCL) and NIST on quartz and thin Spectralon samples.

Measurement of Electron Beam Emittance Using Optical Transition Radiation and Development of a Diffuse Screen Electron Beam Monitor

DTIC Science & Technology

1990-12-01

Zerodur ,irror, 2" relfects light. 1OZ20BD.1; 20th wave zerodur mirror , 1" reflects light. LS-35; 3’ x 5’ optical breadboard; for mounting components...profile measurements using the diffuse screen were compared with measurements using a front surface mirror and a fluorescent screen. The 20 DISTRIBUTION...Beam current and profile measurements using the diffuse screen were compared with measurements using a front surface mirror and a fluorescent screen

Visible to Near-infrared Spectral Reflectance, NGEE-Arctic Tram, Barrow, Alaska, 2015-2017

DOE Office of Scientific and Technical Information (OSTI.GOV)

Meng, Ran; McMahon, Andrew; Rogers, Alistair

Canopy spectral reflectance collected from the NGEE-Arctic automated tram platform using a PP-Systems UniSpec-DC spectrometer. Downwelling radiance was measured using a 2 meter fiber optic cable connected to a cosine diffuser. Upwelling (i.e. reflected) radiance was measured using a 2 meter cable connected to a 12 degree field-of-view (FOV) lens. Canopy reflectance was calculated using the ratio of upwelling to downwelling radiance measured over a 99.99% reflectance Spectralon standard measured at the start of each measurement set.

Combination of acoustical radiosity and the image source method.

PubMed

Koutsouris, Georgios I; Brunskog, Jonas; Jeong, Cheol-Ho; Jacobsen, Finn

2013-06-01

A combined model for room acoustic predictions is developed, aiming to treat both diffuse and specular reflections in a unified way. Two established methods are incorporated: acoustical radiosity, accounting for the diffuse part, and the image source method, accounting for the specular part. The model is based on conservation of acoustical energy. Losses are taken into account by the energy absorption coefficient, and the diffuse reflections are controlled via the scattering coefficient, which defines the portion of energy that has been diffusely reflected. The way the model is formulated allows for a dynamic control of the image source production, so that no fixed maximum reflection order is required. The model is optimized for energy impulse response predictions in arbitrary polyhedral rooms. The predictions are validated by comparison with published measured data for a real music studio hall. The proposed model turns out to be promising for acoustic predictions providing a high level of detail and accuracy.

In vivo assessment of liver fibrosis using diffuse reflectance and fluorescence spectroscopy: a proof of concept.

PubMed

Fabila, Diego; de la Rosa, José Manuel; Stolik, Suren; Moreno, Edgard; Suárez-Álvarez, Karina; López-Navarrete, Giuliana; Guzmán, Carolina; Aguirre-García, Jesús; Acevedo-García, Christian; Kershenobich, David; Escobedo, Galileo

2012-12-01

A novel application of diffuse reflectance and fluorescence spectroscopy in the assessment of liver fibrosis is here reported. To induce different stages of liver fibrosis, a sufficient number of male Wistar rats were differentially exposed to chronic administration with carbon tetrachloride. Then, diffuse reflectance and fluorescence spectra were in vivo measured from the liver surface of each animal by a minimal invasive laparoscopic procedure. The liver fibrosis degree was conventionally determined by means of histological examination using the Mason's Trichrome stain, accompanied by hepatic expression of α-sma, and evaluation of the ALT/AST serum levels. The liver from rats exhibiting higher grades of fibrosis showed a significant increase in diffuse reflectance and fluorescence intensity when compared with control animals. At 365 nm, the diffuse reflectance spectrum exhibited an increase of 4 and 3-fold in mild and advanced fibrotic rats, respectively, when compared to the control group. Similarly, the fluorescence emission at 493 nm was 2-fold higher in fibrotic animals than in controls. By using fluorescence intensity, discrimination algorithms indicated 73% sensitivity and 94% specificity for recognition of hepatic fibrosis, while for diffuse reflectance, these values increased up to 85% and 100%, respectively. Taking into consideration there is a special need for developing new diagnostic approaches focused on detecting different stages of liver fibrosis with minimal invasiveness, these results suggest that diffuse reflectance and fluorescence spectroscopy could be worthy of further exploration in patients with liver disease. Copyright © 2012 Elsevier B.V. All rights reserved.

Experimental Investigations on Two Potential Sound Diffuseness Measures in Enclosures

NASA Astrophysics Data System (ADS)

Bai, Xin

This study investigates two different approaches to measure sound field diffuseness in enclosures from monophonic room impulse responses. One approach quantifies sound field diffuseness in enclosures by calculating the kurtosis of the pressure samples of room impulse responses. Kurtosis is a statistical measure that is known to describe the peakedness or tailedness of the distribution of a set of data. High kurtosis indicates low diffuseness of the sound field of interest. The other one relies on multifractal detrended fluctuation analysis which is a way to evaluate the statistical self-affinity of a signal to measure diffuseness. To test these two approaches, room impulse responses are obtained under varied room-acoustic diffuseness configurations, achieved by using varied degrees of diffusely reflecting interior surfaces. This paper will analyze experimentally measured monophonic room impulse responses, and discuss results from these two approaches.

jasonSWIR Calibration of Spectralon Reflectance Factor

NASA Technical Reports Server (NTRS)

Georgiev, Georgi T.; Butler, James J.; Cooksey, Cahterine; Ding, Leibo; Thome, Kurtis J.

2011-01-01

Satellite instruments operating in the reflective solar wavelength region require accurate and precise determination of the Bidirectional Reflectance Factor (BRF) of laboratory-based diffusers used in their pre-flight and on-orbit radiometric calibrations. BRF measurements are required throughout the reflected-solar spectrum from the ultraviolet through the shortwave infrared. Spectralon diffusers are commonly used as a reflectance standard for bidirectional and hemispherical geometries. The Diffuser Calibration Laboratory (DCaL) at NASA's Goddard Space Flight Center is a secondary calibration facility with reflectance measurements traceable to those made by the Spectral Tri-function Automated Reference Reflectometer (STARR) facility at the National Institute of Standards and Technology (NIST). For more than two decades, the DCaL has provided numerous NASA projects with BRF data in the ultraviolet (UV), visible (VIS) and the Near infraRed (NIR) spectral regions. Presented in this paper are measurements of BRF from 1475nm to 1625nm obtained using an indium gallium arsenide detector and a tunable coherent light source. The sample was a 2 inch diameter, 99% white Spectralon target. The BRF results are discussed and compared to empirically generated data from a model based on NIST certified values of 6deg directional/hemispherical spectral reflectance factors from 900nm to 2500nm. Employing a new NIST capability for measuring bidirectional reflectance using a cooled, extended InGaAs detector, BRF calibration measurements of the same sample were also made using NIST's STARR from 1475nm to 1625nm at an incident angle of 0deg and at viewing angles of 40deg, 45deg, and 50deg. The total combined uncertainty for BRF in this ShortWave Infrared (SWIR) range is less than 1%. This measurement capability will evolve into a BRF calibration service in SWIR region in support of NASA remote sensing missions. Keywords: BRF, BRDF, Calibration, Spectralon, Reflectance, Remote Sensing.

Critical comparison of diffuse reflectance spectroscopy and colorimetry as dermatological diagnostic tools for acanthosis nigricans: a chemometric approach

PubMed Central

Devpura, Suneetha; Pattamadilok, Bensachee; Syed, Zain U.; Vemulapalli, Pranita; Henderson, Marsha; Rehse, Steven J.; Hamzavi, Iltefat; Lim, Henry W.; Naik, Ratna

2011-01-01

Quantification of skin changes due to acanthosis nigricans (AN), a disorder common among insulin-resistant diabetic and obese individuals, was investigated using two optical techniques: diffuse reflectance spectroscopy (DRS) and colorimetry. Measurements were obtained from AN lesions on the neck and two control sites of eight AN patients. A principal component/discriminant function analysis successfully differentiated between AN lesion and normal skin with 87.7% sensitivity and 94.8% specificity in DRS measurements and 97.2% sensitivity and 96.4% specificity in colorimetry measurements. PMID:21698027

Deterioration of reflecting coatings by intermetallic diffusion.

PubMed

Hunter, W R; Mikes, T L; Hass, G

1972-07-01

Gold diffraction gratings overcoated with Al + MgF(2) to increase their efficiency in the vacuum ultraviolet suffered a severe loss in efficiency within six months to a year after coating; for example, from 50% to 2% at lambda1216 A. The cause of this loss was assumed to be interdiffusion of Au and Al; therefore, a more complete study of Au-Al film combinations was performed. The coatings were aged at room and elevated temperatures. Reflectance measurements were made in the visible and vacuum ultraviolet spectral regions. For wavelengths longer than lambda900 A, the measurements show very little change until the diffusion boundary reaches the penetration depth of the radiation. If Al is the first surface layer, however, reflectance measurements at lambda584 A permit measuring the progress of the diffusion boundary toward the Al surface because of the low absorptance of Al at this wavelength. Interdiffusion can be effectively eliminated by the use of thin dielectric layers uch as SiO and the natural oxide of Al. Such protected coatings have been exposed for one week at a temperature of 170 degrees C with no visible sign of diffusion, whereas a similar coating without the barrier layer would become useless in less than 1 h. Some preliminary studies have been made with Pt-Al film combinations.

Quantitative analysis of binary polymorphs mixtures of fusidic acid by diffuse reflectance FTIR spectroscopy, diffuse reflectance FT-NIR spectroscopy, Raman spectroscopy and multivariate calibration.

PubMed

Guo, Canyong; Luo, Xuefang; Zhou, Xiaohua; Shi, Beijia; Wang, Juanjuan; Zhao, Jinqi; Zhang, Xiaoxia

2017-06-05

Vibrational spectroscopic techniques such as infrared, near-infrared and Raman spectroscopy have become popular in detecting and quantifying polymorphism of pharmaceutics since they are fast and non-destructive. This study assessed the ability of three vibrational spectroscopy combined with multivariate analysis to quantify a low-content undesired polymorph within a binary polymorphic mixture. Partial least squares (PLS) regression and support vector machine (SVM) regression were employed to build quantitative models. Fusidic acid, a steroidal antibiotic, was used as the model compound. It was found that PLS regression performed slightly better than SVM regression in all the three spectroscopic techniques. Root mean square errors of prediction (RMSEP) were ranging from 0.48% to 1.17% for diffuse reflectance FTIR spectroscopy and 1.60-1.93% for diffuse reflectance FT-NIR spectroscopy and 1.62-2.31% for Raman spectroscopy. The results indicate that diffuse reflectance FTIR spectroscopy offers significant advantages in providing accurate measurement of polymorphic content in the fusidic acid binary mixtures, while Raman spectroscopy is the least accurate technique for quantitative analysis of polymorphs. Copyright © 2017 Elsevier B.V. All rights reserved.

Spectrophotometric Method for Differentiation of Human Skin Melanoma. I. Optical Diffuse Reflection Coefficient

NASA Astrophysics Data System (ADS)

Petruk, V. G.; Ivanov, A. P.; Kvaternyuk, S. M.; Barun, V. V.

2016-03-01

We have designed an experimental setup, based on two integrating spheres, that lets us measure the optical diffuse reflectance spectra (diffuse reflection coefficient vs. wavelength) of human skin quickly under clinical conditions in vivo. For the wavelength interval 520-1100 nm, we give the values of the diffuse reflection coefficient for healthy tissue, skin with a benign nevus, and skin with a malignant melanoma for a large group of test subjects. We experimentally established a number of wavelengths in the red-near IR region of the spectrum which can be used for early differential diagnosis of nevi and melanoma in patient cancer screening. According to the Kramer-Welch test, the probability of the diffuse reflection coefficient for skin with melanoma and a nevus having different distributions is >0.94, and at many wavelengths it is >0.999. By solving the inverse problem, we estimated the changes in a number of structural and biophysical parameters of the tissue on going from healthy skin to nevus and melanoma. The results obtained can provide a basis for developing a clinical approach to identifying the risk of malignant transformation of the skin before surgery and histological analysis of the tissue.

[Achievement of the noninvasive measurement for human blood glucose with NIR diffusion reflectance spectrum method].

PubMed

Zhang, Hong-yan; Ding, Dong; Song, Li-qiang; Gu, Lin-na; Yang, Peng; Tang, Yu-guo

2005-06-01

The noninvasive measurement of human blood glucose was achieved with NIR diffusion reflectance spectrum method. The thumb fingertip NIR diffusion reflectance spectra of six different age healthy volunteers were collected using Nexus-870 and its NIR fiber port smart accessory. The test was implemented with changing the blood glucose concentration for the limosis and satiation of every volunteer. The calibration model was set up using PLS method with the smoothing, baseline correction and first derivatives pretreatment spectrum in the 7500-8500 cm(-1) region for single volunteer, the same age combination and that of different age. When the spectrum was obtained, the actual blood glucose value of every spectrun sample was demarcated using ultraviolet spectrophotometer. The correlation between the calibration value and true value for single volunteer is better than that for the combination of volunteers, the correlative coefficients are all over 0.90471, RMSECs are all less than 0.171.

PRELIMINARY RESULTS OF BTDF CALIBRATION OF TRANSMISSIVE SOLAR DIFFUSERS FOR REMOTE SENSING.

PubMed

Georgiev, Georgi T; Butler, James J; Thome, Kurt; Cooksey, Catherine; Ding, Leibo

2016-01-01

Satellite instruments operating in the reflected solar wavelength region require accurate and precise determination of the optical properties of their diffusers used in pre-flight and post-flight calibrations. The majority of recent and current space instruments use reflective diffusers. As a result, numerous Bidirectional Reflectance Distribution Function (BRDF) calibration comparisons have been conducted between the National Institute of Standards and Technology (NIST) and other industry and university-based metrology laboratories. However, based on literature searches and communications with NIST and other laboratories, no Bidirectional Transmittance Distribution Function (BTDF) measurement comparisons have been conducted between National Measurement Laboratories (NMLs) and other metrology laboratories. On the other hand, there is a growing interest in the use of transmissive diffusers in the calibration of satellite, air-borne, and ground-based remote sensing instruments. Current remote sensing instruments employing transmissive diffusers include the Ozone Mapping and Profiler Suite instrument (OMPS) Limb instrument on the Suomi-National Polar-orbiting Partnership (S-NPP) platform,, the Geostationary Ocean Color Imager (GOCI) on the Korea Aerospace Research Institute's (KARI) Communication, Ocean, and Meteorological Satellite (COMS), the Ozone Monitoring Instrument (OMI) on NASA's Earth Observing System (EOS) Aura platform, the Tropospheric Emissions: Monitoring of Pollution (TEMPO) instrument and the Geostationary Environmental Monitoring Spectrometer (GEMS).. This ensemble of instruments requires validated BTDF measurements of their on-board transmissive diffusers from the ultraviolet through the near infrared. This paper presents the preliminary results of a BTDF comparison between the NASA Diffuser Calibration Laboratory (DCL) and NIST on quartz and thin Spectralon samples.

Preliminary Results of BTDF Calibration of Transmissive Solar Diffusers for Remote Sensing

NASA Technical Reports Server (NTRS)

Georgiev, Georgi T.; Butler, James J.; Thome, Kurt; Cooksey, Catherine; Ding, Leibo

2016-01-01

Satellite instruments operating in the reflected solar wavelength region require accurate and precise determination of the optical properties of their diffusers used in pre-flight and post-flight calibrations. The majority of recent and current space instruments use reflective diffusers. As a result, numerous Bidirectional Reflectance Distribution Function (BRDF) calibration comparisons have been conducted between the National Institute of Standards and Technology (NIST) and other industry and university-based metrology laboratories. However, based on literature searches and communications with NIST and other laboratories, no Bidirectional Transmittance Distribution Function (BTDF) measurement comparisons have been conducted between National Measurement Laboratories (NMLs) and other metrology laboratories. On the other hand, there is a growing interest in the use of transmissive diffusers in the calibration of satellite, air-borne, and ground-based remote sensing instruments. Current remote sensing instruments employing transmissive diffusers include the Ozone Mapping and Profiler Suite instrument (OMPS) Limb instrument on the Suomi-National Polar-orbiting Partnership (S-NPP) platform,, the Geostationary Ocean Color Imager (GOCI) on the Korea Aerospace Research Institute's (KARI) Communication, Ocean, and Meteorological Satellite (COMS), the Ozone Monitoring Instrument (OMI) on NASA's Earth Observing System (EOS) Aura platform, the Tropospheric Emissions: Monitoring of Pollution (TEMPO) instrument and the Geostationary Environmental Monitoring Spectrometer (GEMS).. This ensemble of instruments requires validated BTDF measurements of their on-board transmissive diffusers from the ultraviolet through the near infrared. This paper presents the preliminary results of a BTDF comparison between the NASA Diffuser Calibration Laboratory (DCL) and NIST on quartz and thin Spectralon samples.

PRELIMINARY RESULTS OF BTDF CALIBRATION OF TRANSMISSIVE SOLAR DIFFUSERS FOR REMOTE SENSING

PubMed Central

Georgiev, Georgi T.; Butler, James J.; Thome, Kurt; Cooksey, Catherine; Ding, Leibo

2016-01-01

Satellite instruments operating in the reflected solar wavelength region require accurate and precise determination of the optical properties of their diffusers used in pre-flight and post-flight calibrations. The majority of recent and current space instruments use reflective diffusers. As a result, numerous Bidirectional Reflectance Distribution Function (BRDF) calibration comparisons have been conducted between the National Institute of Standards and Technology (NIST) and other industry and university-based metrology laboratories. However, based on literature searches and communications with NIST and other laboratories, no Bidirectional Transmittance Distribution Function (BTDF) measurement comparisons have been conducted between National Measurement Laboratories (NMLs) and other metrology laboratories. On the other hand, there is a growing interest in the use of transmissive diffusers in the calibration of satellite, air-borne, and ground-based remote sensing instruments. Current remote sensing instruments employing transmissive diffusers include the Ozone Mapping and Profiler Suite instrument (OMPS) Limb instrument on the Suomi-National Polar-orbiting Partnership (S-NPP) platform,, the Geostationary Ocean Color Imager (GOCI) on the Korea Aerospace Research Institute’s (KARI) Communication, Ocean, and Meteorological Satellite (COMS), the Ozone Monitoring Instrument (OMI) on NASA’s Earth Observing System (EOS) Aura platform, the Tropospheric Emissions: Monitoring of Pollution (TEMPO) instrument and the Geostationary Environmental Monitoring Spectrometer (GEMS).. This ensemble of instruments requires validated BTDF measurements of their on-board transmissive diffusers from the ultraviolet through the near infrared. This paper presents the preliminary results of a BTDF comparison between the NASA Diffuser Calibration Laboratory (DCL) and NIST on quartz and thin Spectralon samples. PMID:28003712

Accelerated rescaling of single Monte Carlo simulation runs with the Graphics Processing Unit (GPU).

PubMed

Yang, Owen; Choi, Bernard

2013-01-01

To interpret fiber-based and camera-based measurements of remitted light from biological tissues, researchers typically use analytical models, such as the diffusion approximation to light transport theory, or stochastic models, such as Monte Carlo modeling. To achieve rapid (ideally real-time) measurement of tissue optical properties, especially in clinical situations, there is a critical need to accelerate Monte Carlo simulation runs. In this manuscript, we report on our approach using the Graphics Processing Unit (GPU) to accelerate rescaling of single Monte Carlo runs to calculate rapidly diffuse reflectance values for different sets of tissue optical properties. We selected MATLAB to enable non-specialists in C and CUDA-based programming to use the generated open-source code. We developed a software package with four abstraction layers. To calculate a set of diffuse reflectance values from a simulated tissue with homogeneous optical properties, our rescaling GPU-based approach achieves a reduction in computation time of several orders of magnitude as compared to other GPU-based approaches. Specifically, our GPU-based approach generated a diffuse reflectance value in 0.08ms. The transfer time from CPU to GPU memory currently is a limiting factor with GPU-based calculations. However, for calculation of multiple diffuse reflectance values, our GPU-based approach still can lead to processing that is ~3400 times faster than other GPU-based approaches.

Computation of diffuse sky irradiance from multidirectional radiance measurements

NASA Technical Reports Server (NTRS)

Ahmad, Suraiya P.; Middleton, Elizabeth M.; Deering, Donald W.

1987-01-01

Accurate determination of the diffuse solar spectral irradiance directly above the land surface is important in characterizing the reflectance properties of these surfaces, especially vegetation canopies. This determination is also needed to infer the net radiation budget of the earth-atmosphere system above these surfaces. An algorithm is developed here for the computation of hemispheric diffuse irradiance using the measurements from an instrument called PARABOLA, which rapidly measures upwelling and downwelling radiances in three selected wavelength bands. The validity of the algorithm is established from simulations. The standard reference data set of diffuse radiances of Dave (1978), obtained by solving the radiative transfer equation numerically for realistic atmospheric models, is used to simulate PARABOLA radiances. Hemispheric diffuse irradiance is estimated from a subset of simulated radiances by using the algorithm described. The algorithm is validated by comparing the estimated diffuse irradiance with the true diffuse irradiance of the standard data set. The validations include sensitivity studies for two wavelength bands (visible, 0.65-0.67 micron; near infrared, 0.81-0.84 micron), different atmospheric conditions, solar elevations, and surface reflectances. In most cases the hemispheric diffuse irradiance computed from simulated PARABOLA radiances and the true irradiance obtained from radiative transfer calculations agree within 1-2 percent. This technique can be applied to other sampling instruments designed to estimate hemispheric diffuse sky irradiance.

Determination of the Ability to Measure Traces of Water in Dehydrated Residues of Waste Water by IR Diffuse Reflectance Spectra

NASA Astrophysics Data System (ADS)

Pratsenka, S. V.; Voropai, E. S.; Belkin, V. G.

2018-01-01

Rapid measurement of the moisture content of dehydrated residues is a critical problem, the solution of which will increase the efficiency of treatment facilities and optimize the process of applying flocculants. The ability to determine the moisture content of dehydrated residues using a meter operating on the IR reflectance principle was confirmed experimentally. The most suitable interference filters were selected based on an analysis of the obtained diffuse reflectance spectrum of the dehydrated residue in the range 1.0-2.7 μm. Calibration curves were constructed and compared for each filter set. A measuring filter with a transmittance maximum at 1.19 μm and a reference filter with a maximum at 1.3 μm gave the best agreement with the laboratory measurements.

Influence of probe pressure on diffuse reflectance spectra of human skin measured in vivo

NASA Astrophysics Data System (ADS)

Popov, Alexey P.; Bykov, Alexander V.; Meglinski, Igor V.

2017-11-01

Mechanical pressure superficially applied on the human skin surface by a fiber-optic probe influences the spatial distribution of blood within the cutaneous tissues. Upon gradual load of weight on the probe, a stepwise increase in the skin reflectance spectra is observed. The decrease in the load follows the similar inverse staircase-like tendency. The observed stepwise reflectance spectra changes are due to, respectively, sequential extrusion of blood from the topical cutaneous vascular beds and their filling afterward. The obtained results are confirmed by Monte Carlo modeling. This implies that pressure-induced influence during the human skin diffuse reflectance spectra measurements in vivo should be taken into consideration, in particular, in the rapidly developing area of wearable gadgets for real-time monitoring of various human body parameters.

Directional Degradation of Spectralon Diffuser Under Ionizing Radiation for Calibration of Space-Based Sensors

NASA Technical Reports Server (NTRS)

Georgiev, G. T.; Butler, J. J.; Kowalewski, M. G.; Ding, L.

2012-01-01

Assessment of the effect of Vacuum Ultra Violet (VUV) irradiation on the Bidirectional Reflectance Distribution Function (BRDF) of Spectralon is presented in this paper. The sample was a 99% white Spectralon calibration standard irradiated with VUV source positioned at 60o off the irradiation direction for a total of 20 hours. The BRDF before and after VUV irradiation was measured and compared at number of wavelengths in the UV, VIS and IR. Non-isotropic directional degradation of Spectralon diffuser under ionizing radiation was detected at different BRDF measurement geometries primarily at UV spectral range. The 8o directional/hemispherical reflectance of the same sample was also measured and compared from 200nm to 2500nm. Index Terms BRDF, Reflectance, Multiangular, Spectralon, Remote Sensing

Temperature dependence of Fe/++/ crystal field spectra - Implications to mineralogical mapping of planetary surfaces

NASA Technical Reports Server (NTRS)

Sung, C.-M.; Singer, R. B.; Parkin, K. M.; Burns, R. G.; Osborne, M.

1977-01-01

Results are reported of Fe(++) crystal field spectral measurements for olivines and pyroxenes up to 400 C. The results are correlated with crystal structure data at elevated temperatures, and the validity of remote-sensed identifications of minerals on hot surfaces of the moon and Mercury is assessed. Two techniques were used to obtain spectra of minerals at elevated temperatures using a spectrophotometer. One employed a diamond cell assembly or a specially designed sample holder to measure polarized absorption spectra of heated single crystals. For the other technique, a sample holder was designed to attach to a diffuse reflectance accessory to produce reflectance spectra of heated powdered samples. Polarized absorption spectra of forsterite at 20-400 C are shown in a graph. Other graphs show the temperature dependence of Fe(++) crystal field bands in olivines, the diffuse reflectance spectra of olivine at 40-400 C, the polarization absorption spectra of orthopyroxene at 30-400 C, the diffuse reflectance spectra of pigeonite at 40-400 C, and unpolarized absorption spectra of lunar pyroxene from Apollo 15 rock 15058.

Diffuse reflectance startigraphy - a new method in the study of loess (?)

NASA Astrophysics Data System (ADS)

József, Szeberényi; Balázs, Bradák; Klaudia, Kiss; József, Kovács; György, Varga; Réka, Balázs; Viczián, István

2017-04-01

The different varieties of loess (and intercalated paleosol layers) together constitute one of the most widespread terrestrial sediments, which was deposited, altered, and redeposited in the course of the changing climatic conditions of the Pleistocene. To reveal more information about Pleistocene climate cycles and/or environments the detailed lithostratigraphical subdivision and classification of the loess variations and paleosols are necessary. Beside the numerous method such as various field measurements, semi-quantitative tests and laboratory investigations, diffuse reflectance spectroscopy (DRS) is one of the well applied method on loess/paleosol sequences. Generally, DRS has been used to separate the detrital and pedogenic mineral component of the loess sections by the hematite/goethite ratio. DRS also has been applied as a joint method of various environmental magnetic investigations such as magnetic susceptibility- and isothermal remanent magnetization measurements. In our study the so-called "diffuse reflectance stratigraphy method" were developed. At First, complex mathematical method was applied to compare the results of the spectral reflectance measurements. One of the most preferred multivariate methods is cluster analysis. Its scope is to group and compare the loess variations and paleosol based on the similarity and common properties of their reflectance curves. In the Second, beside the basic subdivision of the profiles by the different reflectance curves of the layers, the most characteristic wavelength section of the reflectance curve was determined. This sections played the most important role during the classification of the different materials of the section. The reflectance value of individual samples, belonged to the characteristic wavelength were depicted in the function of depth and well correlated with other proxies like grain size distribution and magnetic susceptibility data. The results of the correlation showed the significance of the "combined reflectance stratigraphy" as a stratigraphical method and as an environmental proxy also.

Oblique incidence reflectometry: optical models and measurements using a side-viewing gradient index lens-based endoscopic imaging system

NASA Astrophysics Data System (ADS)

Wall, R. Andrew; Barton, Jennifer K.

2014-06-01

A side-viewing, 2.3-mm diameter oblique incidence reflectometry endoscope has been designed to obtain optical property measurements of turbid samples. Light from a single-mode fiber is relayed obliquely onto the tissue with a gradient index lens-based distal optics assembly and the resulting diffuse reflectance profile is imaged and collected with a 30,000 element, 0.72 mm clear aperture fiber bundle. Sampling the diffuse reflectance in two-dimensions allows for fitting of the reflected intensity profile to a well-known theoretical model, permitting the extraction of both absorption and reduced scattering coefficients of the tissue sample. Models and measurements of the endoscopic imaging system are presented in tissue phantoms and in vivo mouse colon, verifying the endoscope's capabilities to accurately measure effective attenuation coefficient and differentiate diseased from normal colon.

Landsat test of diffuse reflectance models for aquatic suspended solids measurement

NASA Technical Reports Server (NTRS)

Munday, J. C., Jr.; Alfoldi, T. T.

1979-01-01

Landsat radiance data were used to test mathematical models relating diffuse reflectance to aquatic suspended solids concentration. Digital CCT data for Landsat passes over the Bay of Fundy, Nova Scotia were analyzed on a General Electric Co. Image 100 multispectral analysis system. Three data sets were studied separately and together in all combinations with and without solar angle correction. Statistical analysis and chromaticity analysis show that a nonlinear relationship between Landsat radiance and suspended solids concentration is better at curve-fitting than a linear relationship. In particular, the quasi-single-scattering diffuse reflectance model developed by Gordon and coworkers is corroborated. The Gordon model applied to 33 points of MSS 5 data combined from three dates produced r = 0.98.

Objective and Subjective Evaluation of Reflecting and Diffusing Surfaces in Auditoria

NASA Astrophysics Data System (ADS)

Cox, Trevor John

Available from UMI in association with The British Library. Requires signed TDF. The performance of reflectors and diffusers used in auditoria have been evaluated both objectively and subjectively. Two accurate systems have been developed to measure the scattering from surfaces via the cross correlation function. These have been used to measure the scattering from plane panels, curved panels and quadratic residue diffusers (QRDs). The scattering measurements have been used to test theoretical prediction methods based on the Helmholtz-Kirchhoff integral equation. Accurate prediction methods were found for all surfaces tested. The limitations of the more approximate methods have been defined. The assumptions behind Schroeder's design of the QRD have been tested and the local reacting admittance assumption found to be valid over a wide frequency range. It was found that the QRD only produces uniform scattering at low frequencies. For an on-axis source the scattering from a curved panel was as good as from a QRD. For an oblique source the QRD produced much more uniform scattering than the curved panel. The subjective measurements evaluated the smallest perceivable change in the early sound field, the part most influenced by reflectors and diffusers. A natural sounding simulation of a concert hall field within an anechoic chamber was used. Standard objective parameters were reasonable values when compared to values found in real halls and subjective preference measurements. A difference limen was measured for early lateral energy fraction (.048 +/-.005); inter aural cross correlation (.075 +/-.008); clarity index (.67 +/-.13 dB); and centre time (8.6 +/- 1.6 ms). It was found that: (i) when changes are made to diffusers and reflectors, changes in spatial impression will usually be larger than those in clarity; and (ii) acousticians can gain most by paying attention to lateral sound in auditoria. It was also found that: (i) diffuse reflections in the early sound field are not perceived differently from specular reflections; and (ii) the initial time delay gap is not significant to listener preference.

Hyperspectral diffuse reflectance for determination of the optical properties of milk and fruit and vegetable juices

NASA Astrophysics Data System (ADS)

Qin, Jianwei; Lu, Renfu

2005-11-01

Absorption and reduced scattering coefficients are two fundamental optical properties for turbid biological materials. This paper presents the technique and method of using hyperspectral diffuse reflectance for fast determination of the optical properties of fruit and vegetable juices and milks. A hyperspectral imaging system was used to acquire spatially resolved steady-state diffuse reflectance over the spectral region between 530 and 900 nm from a variety of fruit and vegetable juices (citrus, grapefruit, orange, and vegetable) and milks with different fat levels (full, skim and mixed). The system collected diffuse reflectance in the source-detector separation range from 1.1 to 10.0 mm. The hyperspectral reflectance data were analyzed by using a diffusion theory model for semi-infinite homogeneous media. The absorption and reduced scattering coefficients of the fruit and vegetable juices and milks were extracted by inverse algorithms from the scattering profiles for wavelengths of 530-900 nm. Values of the absorption and reduced scattering coefficient at 650 nm were highly correlated to the fat content of the milk samples with the correlation coefficient of 0.990 and 0.989, respectively. The hyperspectral imaging technique can be extended to the measurement of other liquid and solid foods in which light scattering is dominant.

Bidirectional reflectance distribution function of diffuse extreme ultraviolet scatterers and extreme ultraviolet baffle materials.

PubMed

Newell, M P; Keski-Kuha, R A

1997-08-01

Bidirectional reflectance distribution function (BRDF) measurements of a number of diffuse extreme ultraviolet (EUV) scatterers and EUV baffle materials have been performed with the Goddard EUV scatterometer. BRDF data are presented for white Spectralon SRS-99 at 121.6 nm; the data exhibit a non-Lambertian nature and a total hemispherical reflectance lower than 0.15. Data are also presented for an evaporated Cu black sample, a black Spectralon SRS-02 sample, and a Martin Optical Black sample at wavelengths of 58.4 and 121.6 nm and for angles of incidence of 15 degrees and 45 degrees. Overall Martin Optical Black exhibited the lowest BRDF characteristic, with a total hemispherical reflectance of the order of 0.01 and measured BRDF values as low as 2 x 10(-3) sr(-1).

Effective emissivities of isothermal blackbody cavities calculated by the Monte Carlo method using the three-component bidirectional reflectance distribution function model.

PubMed

Prokhorov, Alexander

2012-05-01

This paper proposes a three-component bidirectional reflectance distribution function (3C BRDF) model consisting of diffuse, quasi-specular, and glossy components for calculation of effective emissivities of blackbody cavities and then investigates the properties of the new reflection model. The particle swarm optimization method is applied for fitting a 3C BRDF model to measured BRDFs. The model is incorporated into the Monte Carlo ray-tracing algorithm for isothermal cavities. Finally, the paper compares the results obtained using the 3C model and the conventional specular-diffuse model of reflection.

Reconstructing in-vivo reflectance spectrum of pigmented skin lesion by Monte Carlo simulation

NASA Astrophysics Data System (ADS)

Wang, Shuang; He, Qingli; Zhao, Jianhua; Lui, Harvey; Zeng, Haishan

2012-03-01

In dermatology applications, diffuse reflectance spectroscopy has been extensively investigated as a promising tool for the noninvasive method to distinguish melanoma from benign pigmented skin lesion (nevus), which is concentrated with the skin chromophores like melanin and hemoglobin. We carried out a theoretical study to examine melanin distribution in human skin tissue and establish a practical optical model for further pigmented skin investigation. The theoretical simulation was using junctional nevus as an example. A multiple layer skin optical model was developed on established anatomy structures of skin, the published optical parameters of different skin layers, blood and melanin. Monte Carlo simulation was used to model the interaction between excitation light and skin tissue and rebuild the diffuse reflectance process from skin tissue. A testified methodology was adopted to determine melanin contents in human skin based on in vivo diffuse reflectance spectra. The rebuild diffuse reflectance spectra were investigated by adding melanin into different layers of the theoretical model. One of in vivo reflectance spectra from Junctional nevi and their surrounding normal skin was studied by compare the ratio between nevus and normal skin tissue in both the experimental and simulated diffuse reflectance spectra. The simulation result showed a good agreement with our clinical measurements, which indicated that our research method, including the spectral ratio method, skin optical model and modifying the melanin content in the model, could be applied in further theoretical simulation of pigmented skin lesions.

Optical property measurement from layered biological media

NASA Astrophysics Data System (ADS)

Muller, Matthew R.

1998-12-01

Near infrared (NIR) photon reflectance spectroscopy is applied to measurement of blood concentration and its oxygen saturation within biological tissue. The measurement relies upon the changes in photon absorption of hemoglobin in the tissue as changes occur in the hemoglobin concentration and oxygen content. In the present study, NIR light is introduced at the skin surface and the optical properties (absorption and scattering) within the underlying tissue are determined from the resulting surface reflectance. Typically the tissue is modeled as a homogeneous mixture of bloodless tissue and blood, and the model incorporates the physical relationship between the surface reflectance and the optical properties of the tissue. The skin and underlying tissue, although heterogeneous, have a characteristic layered structure. These layers can be differentiated optically. The modeling and the inverse problem of measuring the optical properties in each of the tissue layers from the surface reflectance have been the subject of much attention by a number of investigators. Nonetheless, quantification of the relationship between surface reflectance and the optical properties of layered tissue has not been well understood nor well described. In the forward problem, tissue optical properties yield surface reflectance profiles (SRPs). Surface reflectance profiles, or SRPs, from diffusive media consisting of two layers are calculated using numerical solutions to the Boltzmann equation. Experimental SRPs are also measured in vitro from a test medium and in vivo from the calf of human subjects. This study provides a new approach to solving the inverse problem of determining optical properties from SRPs. To solve the inverse problem, an effective diffusion constant (Ke) is determined for the layered media. The Ke is the diffusion constant of an equivalent homogeneous medium which best fits the SRP of the layered medium. The departure from Ke of the SRP for a layered media is captured concisely, and Ke becomes a tool in describing the layered optical properties. This approach is applied clinically to measure changes in the blood concentration and oxygenation measured in vivo from normals and patients with peripheral vascular disease. A significant finding from the modeling was to identify the functional relationship of Ke to the top and lower layer diffusion constants, and the top layer thickness. When applied to in vitro measurements from media containing homogeneous layers with known optical properties, this functional relationship predicted Ke within the 95% confidence interval of the measured Ke. For the in vivo measurements, changes in K e with exercise are consistent with expected exercise physiology. With the incorporation of the known optical absorbance of hemoglobin in the presence of oxygen, the SRPs provide a means to measure the oxygen saturation of a deep tissue layer from the surface light reflectance.

Cortexin diffusion in human eye sclera

DOE Office of Scientific and Technical Information (OSTI.GOV)

Genina, Elina A; Bashkatov, A N; Tuchin, Valerii V

2011-05-31

Investigation of the diffusion of cytamines, a typical representative of which is cortexin, is important for evaluating the drug dose, necessary to provide sufficient concentration of the preparation in the inner tissues of the eye. In the present paper, the cortexin diffusion rate in the eye sclera is measured using the methods of optical coherence tomography (OCT) and reflectance spectroscopy. The technique for determining the diffusion coefficient is based on the registration of temporal dependence of the eye sclera scattering parameters caused by partial replacement of interstitial fluid with the aqueous cortexin solution, which reduces the level of the OCTmore » signal and decreases the reflectance of the sclera. The values of the cortexin diffusion coefficient obtained using two independent optical methods are in good agreement. (optical technologies in biophysics and medicine)« less

Quantitative interpretations of Visible-NIR reflectance spectra of blood.

PubMed

Serebrennikova, Yulia M; Smith, Jennifer M; Huffman, Debra E; Leparc, German F; García-Rubio, Luis H

2008-10-27

This paper illustrates the implementation of a new theoretical model for rapid quantitative analysis of the Vis-NIR diffuse reflectance spectra of blood cultures. This new model is based on the photon diffusion theory and Mie scattering theory that have been formulated to account for multiple scattering populations and absorptive components. This study stresses the significance of the thorough solution of the scattering and absorption problem in order to accurately resolve for optically relevant parameters of blood culture components. With advantages of being calibration-free and computationally fast, the new model has two basic requirements. First, wavelength-dependent refractive indices of the basic chemical constituents of blood culture components are needed. Second, multi-wavelength measurements or at least the measurements of characteristic wavelengths equal to the degrees of freedom, i.e. number of optically relevant parameters, of blood culture system are required. The blood culture analysis model was tested with a large number of diffuse reflectance spectra of blood culture samples characterized by an extensive range of the relevant parameters.

Multiple-scattering coefficients and absorption controlled diffusive processes

NASA Astrophysics Data System (ADS)

Godoy, Salvador; García-Colín, L. S.; Micenmacher, Victor

1999-11-01

Multiple-scattering transmission and reflection coefficients (T,R) are introduced in addition to the diffusion coefficient D for the description of ballistic diffusion in the presence of absorption. For 1D (one-dimensional) systems, the measurement of only one between T and D imposes restrictions on the possible values of the other. If D is measured, then T is bounded between the Landauer and Lambert-Beer equations. Measurements of both (T,D) imply the theoretical knowledge of the microscopic absorption Σa and scattering rΣs cross sections.

Bidirectional Reflectance Round-Robin in Support of the Earth Observing System Program

NASA Technical Reports Server (NTRS)

Early, E.; Barnes, P.; Johnson, B.; Butler, J.; Bruegge, C.; Biggar, S.; Spyak, P.; Pavlov, M.

1999-01-01

Laboratory measurements of the bidirectional reflectance distribution function (BRDRF) of diffuse reflectors are required to support calibration in the Earth Observing System (EOS) program of the National Aeronautics and Space Administration.

Reflectance spectroscopy for noninvasive evaluation of hair follicle stage

NASA Astrophysics Data System (ADS)

Liu, Caihua; Guan, Yue; Wang, Jianru; Zhong, Xiewei; Liu, Xiuli; Zhu, Dan

2015-05-01

Hair follicle offers an excellent model for systems biology and regenerative medicine. So far, the stages of hair follicle growth have been evaluated by histological examination. In this work, a noninvasive spectroscopy was proposed by measuring the diffuse reflectance of mouse skin and analyzing the melanin value. Results show that the skin diffuse reflectance was relatively high when hair follicles were at the telogen stage and at the beginning of the anagen stage, and decreased with the progression of the anagen stage. When the hair follicle entered into the catagen stage, the diffuse reflectance gradually increased. The changes in the melanin content of skin had contrary dynamics. Substages of the hair follicle cycle could be distinguished by comparing the changes in melanin value with the histological examination. This study provided a new method for noninvasive evaluation of the hair follicle stage, and should be valuable for basic and therapeutic investigations on hair regeneration.

Measurement and Modeling of the Optical Scattering Properties of Crop Canopies

NASA Technical Reports Server (NTRS)

Vanderbilt, V. C.; Grant, L.

1984-01-01

Efforts in measuring, analyzing, and mathematically modeling the specular, polarized, and diffuse light scattering properties of several plant canopies and their component parts (leaves, stems, fruit, soil) as a function of view angle and illumination angle are reported. Specific objectives were: (1) to demonstrate a technique for determining the specular and diffuse components of the reflectance factor of plant canopies; (2) to acquire the measurements and begin assembling a data set for developing and testing canopy reflectance models; (3) to design and build a new optical instrument to measure the light scattering properties of individual leaves; and (4) to use this instrument to survey and investigate the information in the light scattering properties of individual leaves of crops, forests, weeds, and horticulture.

Basic study of charring detection at the laser catheter-tip using back scattering light measurement during therapeutic laser irradiation in blood.

PubMed

Takahashi, Mei; Ito, Arisa; Kajihara, Takuro; Matsuo, Hiroki; Arai, Tsunenori

2010-01-01

The purpose of this study is to investigate transient process of the charring at the laser catheter-tip in blood during therapeutic laser irradiation by the back scattering light measurement to detect precursor state of the charring. We took account of using photodynamic therapy for arrhythmia in blood through the laser catheter. We observed the influence of the red laser irradiation (λ=663 nm) upon the shape of red blood cells (RBCs). The RBCs aggregation, round formation, and hemolysis were took place sequentially before charring. With a model blood sandwiched between glass plates simulated as a catheter-tip boundary, we measured diffuse-reflected-light power and transmitted-light power simultaneously and continuously by a microscopic optics during the laser irradiation. We found that measured light power changes were originated with RBCs shape change induced by temperature rise due to the laser irradiation. A gentle peak following a slow descending was observed in the diffuse-reflected-light power history. This history might indicate the precursor state of the charring, in which the hemolysis might be considered to advance rapidly. We think that the measurement of diffuse-reflected-light power history might be able to detect precursor state of charring at the catheter-tip in blood.

Dual-modality optical biopsy of glioblastomas multiforme with diffuse reflectance and fluorescence: ex vivo retrieval of optical properties

NASA Astrophysics Data System (ADS)

Du Le, Vinh Nguyen; Provias, John; Murty, Naresh; Patterson, Michael S.; Nie, Zhaojun; Hayward, Joseph E.; Farrell, Thomas J.; McMillan, William; Zhang, Wenbin; Fang, Qiyin

2017-02-01

Glioma itself accounts for 80% of all malignant primary brain tumors, and glioblastoma multiforme (GBM) accounts for 55% of such tumors. Diffuse reflectance and fluorescence spectroscopy have the potential to discriminate healthy tissues from abnormal tissues and therefore are promising noninvasive methods for improving the accuracy of brain tissue resection. Optical properties were retrieved using an experimentally evaluated inverse solution. On average, the scattering coefficient is 2.4 times higher in GBM than in low grade glioma (LGG), and the absorption coefficient is 48% higher. In addition, the ratio of fluorescence to diffuse reflectance at the emission peak of 460 nm is 2.6 times higher for LGG while reflectance at 650 nm is 2.7 times higher for GBM. The results reported also show that the combination of diffuse reflectance and fluorescence spectroscopy could achieve sensitivity of 100% and specificity of 90% in discriminating GBM from LGG during ex vivo measurements of 22 sites from seven glioma specimens. Therefore, the current technique might be a promising tool for aiding neurosurgeons in determining the extent of surgical resection of glioma and, thus, improving intraoperative tumor identification for guiding surgical intervention.

Dual-modality optical biopsy of glioblastomas multiforme with diffuse reflectance and fluorescence: ex vivo retrieval of optical properties.

PubMed

Du Le, Vinh Nguyen; Provias, John; Murty, Naresh; Patterson, Michael S; Nie, Zhaojun; Hayward, Joseph E; Farrell, Thomas J; McMillan, William; Zhang, Wenbin; Fang, Qiyin

2017-02-01

Glioma itself accounts for 80% of all malignant primary brain tumors, and glioblastoma multiforme (GBM) accounts for 55% of such tumors. Diffuse reflectance and fluorescence spectroscopy have the potential to discriminate healthy tissues from abnormal tissues and therefore are promising noninvasive methods for improving the accuracy of brain tissue resection. Optical properties were retrieved using an experimentally evaluated inverse solution. On average, the scattering coefficient is 2.4 times higher in GBM than in low grade glioma (LGG), and the absorption coefficient is 48% higher. In addition, the ratio of fluorescence to diffuse reflectance at the emission peak of 460 nm is 2.6 times higher for LGG while reflectance at 650 nm is 2.7 times higher for GBM. The results reported also show that the combination of diffuse reflectance and fluorescence spectroscopy could achieve sensitivity of 100% and specificity of 90% in discriminating GBM from LGG during ex vivo measurements of 22 sites from seven glioma specimens. Therefore, the current technique might be a promising tool for aiding neurosurgeons in determining the extent of surgical resection of glioma and, thus, improving intraoperative tumor identification for guiding surgical intervention.

Polarization Signals of Common Spacecraft Materials

NASA Technical Reports Server (NTRS)

Gravseth, Ian; Culp, Robert D.; King, Nicole

1996-01-01

This is the final report documenting the results of the polarization testing of near-planar objects with various reflectance properties. The purpose of this investigation was to determine the portion of the reflected signal which is polarized for materials commonly used in space applications. Tests were conducted on several samples, with surface characteristics ranging from highly reflective to relatively dark. The measurements were obtained by suspending the test object in a beam of collimated light. The amount of light falling on the sample was controlled by a circular aperture placed in the light field. The polarized reflectance at various phase angles was then measured. A nonlinear least squares fitting program was used for analysis. For the specular test objects, the reflected signals were measured in one degree increments near the specular point. Otherwise, measurements were taken every five degrees in phase angle. Generally, the more diffuse surfaces had lower polarized reflectances than their more specular counterparts. The reflected signals for the more diffuse surfaces were spread over a larger phase angle range, while the signals from the more specular samples were reflected almost entirely within five degrees of angular deviation from the specular point. The method used to test all the surfaces is presented. The results of this study will be used to support the NASA Orbital Debris Optical Signature Tests. These tests are intended to help better understand the reflectance properties of materials often used in space applications. This data will then be used to improve the capabilities for identification and tracking of space debris.

Positional dependence of the SNPP VIIRS SD BRDF degradation factor

NASA Astrophysics Data System (ADS)

Lei, Ning; Chen, Xuexia; Chang, Tiejun; Xiong, Xiaoxiong

2017-09-01

The Visible Infrared Imaging Radiometer Suite (VIIRS) aboard the Suomi National Polar-orbiting Partnership (SNPP) satellite is a passive scanning radiometer and an imager. The VIIRS regularly performs on-orbit radiometric calibration of its reflective solar bands (RSBs) through observing an onboard sunlit solar diffuser (SD). The reflectance of the SD changes over time and the change is denoted as the SD bidirectional reflectance distribution function degradation factor. The degradation factor, measured by an onboard solar diffuser stability monitor, has been shown to be both incident sunlight and outgoing direction dependent. In this Proceeding, we investigate the factor's dependence on SD position. We develop a model to relate the SD degradation factor with the amount of solar exposure. We use Earth measurements to evaluate the effectiveness of the model.

Elimination of single-beam substitution error in diffuse reflectance measurements using an integrating sphere.

PubMed

Vidovic, Luka; Majaron, Boris

2014-02-01

Diffuse reflectance spectra (DRS) of biological samples are commonly measured using an integrating sphere (IS). To account for the incident light spectrum, measurement begins by placing a highly reflective white standard against the IS sample opening and collecting the reflected light. After replacing the white standard with the test sample of interest, DRS of the latter is determined as the ratio of the two values at each involved wavelength. However, such a substitution may alter the fluence rate inside the IS. This leads to distortion of measured DRS, which is known as single-beam substitution error (SBSE). Barring the use of more complex experimental setups, the literature states that only approximate corrections of the SBSE are possible, e.g., by using look-up tables generated with calibrated low-reflectivity standards. We present a practical method for elimination of SBSE when using IS equipped with an additional reference port. Two additional measurements performed at this port enable a rigorous elimination of SBSE. Our experimental characterization of SBSE is replicated by theoretical derivation. This offers an alternative possibility of computational removal of SBSE based on advance characterization of a specific DRS setup. The influence of SBSE on quantitative analysis of DRS is illustrated in one application example.

Diffuse reflectance spectra measured in vivo in human tissues during Photofrin-mediated pleural photodynamic therapy: updated results

NASA Astrophysics Data System (ADS)

Finlay, Jarod C.; Zhu, Timothy C.; Dimofte, Andreea; Friedberg, Joseph S.; Cengel, Keith A.; Hahn, Stephen M.

2009-02-01

We present the results of a series of spectroscopic measurements made in vivo in patients undergoing photodynamic therapy (PDT). The patients studied here were enrolled in Phase II clinical trials of Photofrin-mediated PDT for the treatment of non-small cell lung cancer and cancers with pleural effusion. Patients were given Photofrin at dose of 2 mg per kg body weight 24 hours prior to treatment. Each patient received surgical debulking of the tumor followed by intracavity PDT at 630nm to a dose of 60 J/cm2. Dose was monitored continuously using implanted isotropic fiber-based light detectors. We measured the diffuse reflectance spectra before and after PDT in various positions within the cavity, including tumor, diaphragm, pericardium, skin, and chest wall muscle in 10 patients. The measurements were acquired using a specially designed fiber optic-based probe consisting of one fluorescence excitation fiber, one white light delivery fiber, and 9 detection fibers spaced at distances from 0.36 to 7.8 mm from the source, all of which are imaged via a spectrograph onto a CCD, allowing measurement of radially-resolved diffuse reflectance and fluorescence spectra. The absorption spectra were analyzed using an analytical model of light propagation in diffuse media based on the P3 approximation to radiative transport, assuming a known basis set of absorbers including hemoglobin in its oxygenated and deoxygenated forms and Photofrin. We find significant variation in hemodynamics and sensitizer concentration among patients and within tissues in a single patient.

Development of response models for the Earth Radiation Budget Experiment (ERBE) sensors. Part 2: Analysis of the ERBE integrating sphere ground calibration

NASA Technical Reports Server (NTRS)

Halyo, Nesim; Taylor, Deborah B.

1987-01-01

An explicit solution of the spectral radiance leaving an arbitrary point on the wall of a spherical cavity with diffuse reflectivity is obtained. The solution is applicable to spheres with an arbitrary number of openings of any size and shape, an arbitrary number of light sources with possible non-diffuse characteristics, a non-uniform sphere wall temperature distribution, non-uniform and non-diffuse sphere wall emissivity and non-uniform but diffuse sphere wall spectral reflectivity. A general measurement equation describing the output of a sensor with a given field of view, angular and spectral response measuring the sphere output is obtained. The results are applied to the Earth Radiation Budget Experiment (ERBE) integrating sphere. The sphere wall radiance uniformity, loading effects and non-uniform wall temperature effects are investigated. It is shown that using appropriate interpretation and processing, a high-accuracy short-wave calibration of the ERBE sensors can be achieved.

The effect of weave orientation on the BRDF of tarp samples

NASA Astrophysics Data System (ADS)

Georgiev, Georgi; Butler, James J.

2003-10-01

The results of bi-directional reflectance distribution function (BRDF) measurements of four tarp samples obtained from NASA"s Stennis Space Center (SSC) are presented. The measurements were performed in the Diffuser Calibration Facility (DCaF) at NASA"s Goddard Space Flight Center (GSFC). The samples are of similar material structure but different reflectance. The experimental data were obtained with a Xe arc lamp/monochromator light source as well as laser light sources in the ultraviolet, visible, and near infrared spectral regions. The BRDF data were recorded at four incident zenith angles and at five incident azimuth angles. The dependence of the measured BRDF on weave orientation was analyzed and presented. 8 degree irectional/hemispherical reflectance data were also measured for each tarp sample, and those results are also reported. All results are NIST traceable through calibrated standard plates. The specular and diffuse scatter data obtained from these studies are used by NASA"s SSC in their field-based, vicarious calibration of satellite and airborne remote sensing instruments.

Reflection measurements for luminescent powders

NASA Astrophysics Data System (ADS)

Kroon, R. E.

2018-04-01

Luminescent materials are useful in applications varying from lighting and display technologies to document security features and medical research, amongst many others. Measurement of the excitation range is an important consideration, and absorption bands are often determined from a decrease in the measured diffuse reflectance of the material using a ultraviolet-visible (UV-vis) spectrophotometer with an integrating sphere. Such a system may provide questionable results when used to measure the reflectance of a luminescence material, which is demonstrated for a Tb doped silica phosphor, because the system cannot differentiate between the reflected light and luminescence. It is shown that more reliable results are achieved for this phosphor by measuring the reflectance using a synchronous zero-offset scan in a fluorescence spectrometer equipped with an integrating sphere. This method is therefore recommended instead of traditional reflectance measurements using a UV-vis spectrophotometer for luminescent powders.

Spectral distribution of UV range diffuse reflectivity for Si+ ion implanted polymers

NASA Astrophysics Data System (ADS)

Balabanov, S.; Tsvetkova, T.; Borisova, E.; Avramov, L.; Bischoff, L.

2008-05-01

The analysis of the UV range spectral characteristics can supply additional information on the formed sub-surface buried layer with implanted dopants. The near-surface layer (50÷150 nm) of bulk polymer samples have been implanted with silicon (Si+) ions at low energies (E = 30 keV) and a wide range of ion doses (D = 1.1013 ÷ 1, 2.1017 cm-2). The studied polymer materials were: ultra-high-molecular-weight polyethylene (UHMWPE), poly-methyl-metacrylate (PMMA) and poly-tetra-fluor-ethylene (PTFE). The diffuse optical reflectivity spectra Rd = f(λ) of the ion implanted samples have been measured in the UV range (λ = 220÷350 nm). In this paper the dose dependences of the size and sign of the diffuse optical reflectivity changes λRd = f(D) have been analysed.

Neutron Reflectivity Measurement for Polymer Dynamics near Graphene Oxide Monolayers

NASA Astrophysics Data System (ADS)

Koo, Jaseung

We investigated the diffusion dynamics of polymer chains confined between graphene oxide layers using neutron reflectivity (NR). The bilayers of polymethylmetacrylate (PMMA)/ deuterated PMMA (d-PMMA) films and polystyrene (PS)/d-PS films with various film thickness sandwiched between Langmuir-Blodgett (LB) monolayers of graphene oxide (GO) were prepared. From the NR results, we found that PMMA diffusion dynamics was reduced near the GO surface while the PS diffusion was not significantly changed. This is due to the different strength of GO-polymer interaction. In this talk, these diffusion results will be compared with dewetting dynamics of polymer thin films on the GO monolayers. This has given us the basis for development of graphene-based nanoelectronics with high efficiency, such as heterojunction devices for polymer photovoltaic (OPV) applications.

Preliminary evaluation of optical glucose sensing in red cell concentrations using near-infrared diffuse-reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Suzuki, Yusuke; Maruo, Katsuhiko; Zhang, Alice W.; Shimogaki, Kazushige; Ogawa, Hideto; Hirayama, Fumiya

2012-01-01

Bacterial contamination of blood products is one of the most frequent infectious complications of transfusion. Since glucose levels in blood supplies decrease as bacteria proliferate, it should be possible to detect the presence of bacterial contamination by measuring the glucose concentrations in the blood components. Hence this study is aimed to serve as a preliminary study for the nondestructive measurement of glucose level in transfusion blood. The glucose concentrations in red blood cell (RBC) samples were predicted using near-infrared diffuse-reflectance spectroscopy in the 1350 to 1850 nm wavelength region. Furthermore, the effects of donor, hematocrit level, and temperature variations among the RBC samples were observed. Results showed that the prediction performance of a dataset which contained samples that differed in all three parameters had a standard error of 29.3 mg/dL. Multiplicative scatter correction (MSC) preprocessing method was also found to be effective in minimizing the variations in scattering patterns created by various sample properties. The results suggest that the diffuse-reflectance spectroscopy may provide another avenue for the detection of bacterial contamination in red cell concentrations (RCC) products.

Fiber-based modulated optical reflectance configuration allowing for offset pump and probe beams

NASA Astrophysics Data System (ADS)

Fleming, A.; Folsom, C.; Jensen, C.; Ban, H.

2016-12-01

A new fiber-based modulated optical reflectance configuration is developed in this work. The technique maintains the fiber-based heating laser (pump) and detection laser (probe) in close proximity at a fixed separation distance in a ceramic ferrule. The pump beam periodically heats the sample inducing thermal waves into the sample. The probe beam measures the temperature response at a known distance from the pump beam over a range of heating modulation frequencies. The thermal diffusivity of the sample may be calculated from the phase response between the input heat flux and the temperature response of a sample having a reflective surface. The unique measurement configuration is ideal for in situ measurements and has many advantages for laboratory-based systems. The design and development of the system are reported along with theoretical justification for the experimental design. The thermal diffusivities of Ge and SiC are measured and found to be within 10% of reported literature values. The diffusivity for SiO2 is measured with a relative difference of approximately 100% from the literature value when the ferrule is in contact with the sample. An additional measurement was made on the SiO2 sample with the ferrule not in contact resulting in a difference of less than 2% from the literature value. The difference in the SiO2 measurement when the ferrule is in contact with the sample is likely due to a parallel heat transfer path through the dual-fiber ferrule assembly.

Fiber-based modulated optical reflectance configuration allowing for offset pump and probe beams.

PubMed

Fleming, A; Folsom, C; Jensen, C; Ban, H

2016-12-01

A new fiber-based modulated optical reflectance configuration is developed in this work. The technique maintains the fiber-based heating laser (pump) and detection laser (probe) in close proximity at a fixed separation distance in a ceramic ferrule. The pump beam periodically heats the sample inducing thermal waves into the sample. The probe beam measures the temperature response at a known distance from the pump beam over a range of heating modulation frequencies. The thermal diffusivity of the sample may be calculated from the phase response between the input heat flux and the temperature response of a sample having a reflective surface. The unique measurement configuration is ideal for in situ measurements and has many advantages for laboratory-based systems. The design and development of the system are reported along with theoretical justification for the experimental design. The thermal diffusivities of Ge and SiC are measured and found to be within 10% of reported literature values. The diffusivity for SiO 2 is measured with a relative difference of approximately 100% from the literature value when the ferrule is in contact with the sample. An additional measurement was made on the SiO 2 sample with the ferrule not in contact resulting in a difference of less than 2% from the literature value. The difference in the SiO 2 measurement when the ferrule is in contact with the sample is likely due to a parallel heat transfer path through the dual-fiber ferrule assembly.

The role of diffusive architectural surfaces on auditory spatial discrimination in performance venues.

PubMed

Robinson, Philip W; Pätynen, Jukka; Lokki, Tapio; Jang, Hyung Suk; Jeon, Jin Yong; Xiang, Ning

2013-06-01

In musical or theatrical performance, some venues allow listeners to individually localize and segregate individual performers, while others produce a well blended ensemble sound. The room acoustic conditions that make this possible, and the psycho-acoustic effects at work are not fully understood. This research utilizes auralizations from measured and simulated performance venues to investigate spatial discrimination of multiple acoustic sources in rooms. Signals were generated from measurements taken in a small theater, and listeners in the audience area were asked to distinguish pairs of speech sources on stage with various spatial separations. This experiment was repeated with the proscenium splay walls treated to be flat, diffusive, or absorptive. Similar experiments were conducted in a simulated hall, utilizing 11 early reflections with various characteristics, and measured late reverberation. The experiments reveal that discriminating the lateral arrangement of two sources is possible at narrower separation angles when reflections come from flat or absorptive rather than diffusive surfaces.

Portable, Fiber-Based, Diffuse Reflection Spectroscopy (DRS) Systems for Estimating Tissue Optical Properties.

PubMed

Vishwanath, Karthik; Chang, Kevin; Klein, Daniel; Deng, Yu Feng; Chang, Vivide; Phelps, Janelle E; Ramanujam, Nimmi

2011-02-01

Steady-state diffuse reflection spectroscopy is a well-studied optical technique that can provide a noninvasive and quantitative method for characterizing the absorption and scattering properties of biological tissues. Here, we compare three fiber-based diffuse reflection spectroscopy systems that were assembled to create a light-weight, portable, and robust optical spectrometer that could be easily translated for repeated and reliable use in mobile settings. The three systems were built using a broadband light source and a compact, commercially available spectrograph. We tested two different light sources and two spectrographs (manufactured by two different vendors). The assembled systems were characterized by their signal-to-noise ratios, the source-intensity drifts, and detector linearity. We quantified the performance of these instruments in extracting optical properties from diffuse reflectance spectra in tissue-mimicking liquid phantoms with well-controlled optical absorption and scattering coefficients. We show that all assembled systems were able to extract the optical absorption and scattering properties with errors less than 10%, while providing greater than ten-fold decrease in footprint and cost (relative to a previously well-characterized and widely used commercial system). Finally, we demonstrate the use of these small systems to measure optical biomarkers in vivo in a small-animal model cancer therapy study. We show that optical measurements from the simple portable system provide estimates of tumor oxygen saturation similar to those detected using the commercial system in murine tumor models of head and neck cancer.

Reflection Matrix Method for Controlling Light After Reflection From a Diffuse Scattering Surface

DTIC Science & Technology

2016-12-22

reflective inverse diffusion, which was a proof-of-concept experiment that used phase modulation to shape the wavefront of a laser causing it to refocus...after reflection from a rough surface. By refocusing the light, reflective inverse diffusion has the potential to eliminate the complex radiometric model...photography. However, the initial reflective inverse diffusion experiments provided no mathematical background and were conducted under the premise that the

Spectral measurements and analyses of atmospheric effects on remote sensor data

NASA Technical Reports Server (NTRS)

Hulstrom, R. L.

1975-01-01

The radiance as measured by a satellite remote sensor is determined by a number of different factors, including the intervening atmosphere, the target reflectivity characteristics, the characteristics of the total incident solar irradiance, and the incident solar irradiance/sensor viewing geometry. Measurement techniques and instrumentation are considered, taking into account total and diffuse solar irradiance, target reflectance/radiance, atmospheric optical depth/transmittance, and atmospheric path radiance.

On measurements of Effective Residual Ink Concentration (ERIC) of deinked papers using Kubelka-Munk theory

Treesearch

D.W. Vahey; J.Y. Zhu; C.J. Houtman

2006-01-01

The measurement of effective residual ink concentration (ERIC) in recycled papers depends on their opacity. For opacity less than 97.0%, the method is based on application of the Kubelka-Munk theory to diffuse reflection from papers measured once with a black backing and again with a thick backing of the same papers. At opacities above 97.0%, the two reflection values...

Application of the specular and diffuse reflection analysis for in vitro diagnostics of dental erosion: correlation with enamel softening, roughness, and calcium release

PubMed Central

Rakhmatullina, Ekaterina; Bossen, Anke; Höschele, Christoph; Wang, Xiaojie; Beyeler, Barbara; Meier, Christoph; Lussi, Adrian

2011-01-01

We present assembly and application of an optical reflectometer for the analysis of dental erosion. The erosive procedure involved acid-induced softening and initial substance loss phases, which are considered to be difficult for visual diagnosis in a clinic. Change of the specular reflection signal showed the highest sensitivity for the detection of the early softening phase of erosion among tested methods. The exponential decrease of the specular reflection intensity with erosive duration was compared to the increase of enamel roughness. Surface roughness was measured by optical analysis, and the observed tendency was correlated with scanning electron microscopy images of eroded enamel. A high correlation between specular reflection intensity and measurement of enamel softening (r2 ≥ −0.86) as well as calcium release (r2 ≥ −0.86) was found during erosion progression. Measurement of diffuse reflection revealed higher tooth-to-tooth deviation in contrast to the analysis of specular reflection intensity and lower correlation with other applied methods (r2 = 0.42–0.48). The proposed optical method allows simple and fast surface analysis and could be used for further optimization and construction of the first noncontact and cost-effective diagnostic tool for early erosion assessment in vivo. PMID:22029364

Application of the specular and diffuse reflection analysis for in vitro diagnostics of dental erosion: correlation with enamel softening, roughness, and calcium release

NASA Astrophysics Data System (ADS)

Rakhmatullina, Ekaterina; Bossen, Anke; Höschele, Christoph; Wang, Xiaojie; Beyeler, Barbara; Meier, Christoph; Lussi, Adrian

2011-10-01

We present assembly and application of an optical reflectometer for the analysis of dental erosion. The erosive procedure involved acid-induced softening and initial substance loss phases, which are considered to be difficult for visual diagnosis in a clinic. Change of the specular reflection signal showed the highest sensitivity for the detection of the early softening phase of erosion among tested methods. The exponential decrease of the specular reflection intensity with erosive duration was compared to the increase of enamel roughness. Surface roughness was measured by optical analysis, and the observed tendency was correlated with scanning electron microscopy images of eroded enamel. A high correlation between specular reflection intensity and measurement of enamel softening (r2 >= -0.86) as well as calcium release (r2 >= -0.86) was found during erosion progression. Measurement of diffuse reflection revealed higher tooth-to-tooth deviation in contrast to the analysis of specular reflection intensity and lower correlation with other applied methods (r2 = 0.42-0.48). The proposed optical method allows simple and fast surface analysis and could be used for further optimization and construction of the first noncontact and cost-effective diagnostic tool for early erosion assessment in vivo.

The contribution of the diffuse light component to the topographic effect on remotely sensed data

NASA Technical Reports Server (NTRS)

Justice, C.; Holben, B.

1980-01-01

The topographic effect is measured by the difference between the global radiance from inclined surfaces as a function of their orientation relative to the sensor position and light source. The short wave radiant energy incident on a surface is composed of direct sunlight, scattered skylight, and light reflected from surrounding terrain. The latter two components are commonly known as the diffuse component. The contribution of the diffuse light component to the topographic effect was examined and the significance of this diffuse component with respect to two direct radiance models was assessed. Diffuse and global spectral radiances were measured for a series of slopes and aspects of a uniform and surface in the red and photographic infrared parts of the spectrum, using a nadir pointing two channel handheld radiometer. The diffuse light was found to produce a topographic effect which varied from the topographic effect for direct light. The topographic effect caused by diffuse light was found to increase slightly with solar elevation and wavelength for the channels examined. The correlations between data derived from two simple direct radiance simulation models and the field data were not significantly affected when the diffuse component was removed from the radiances. Radiances from a 60 percent reflective surface, assuming no atmospheric path radiance, the diffuse light topographic effect contributed a maximum range of 3 pixel values in simulated LANDSAT data from all aspects with slopes up to 30 degrees.

Time-dependent diffuse reflectance spectroscopy for in vivo characterization of pediatric epileptogenic brain lesions

NASA Astrophysics Data System (ADS)

Oh, Sanghoon; Ragheb, John; Bhatia, Sanjiv; Sandberg, David; Johnson, Mahlon; Fernald, Bradley; Lin, Wei-Chiang

2008-02-01

Optical spectroscopy for in vivo tissue diagnosis is performed traditionally in a static manner; a snap shot of the tissue biochemical and morphological characteristics is captured through the interaction between light and the tissue. This approach does not capture the dynamic nature of a living organ, which is critical to the studies of brain disorders such as epilepsy. Therefore, a time-dependent diffuse reflectance spectroscopy system with a fiber-optic probe was designed and developed. The system was designed to acquire broadband diffuse reflectance spectra (240 ~ 932 nm) at an acquisition rate of 33 Hz. The broadband spectral acquisition feature allows simultaneous monitoring of various physiological characteristics of tissues. The utility of such a system in guiding pediatric epilepsy surgery was tested in a pilot clinical study including 13 epilepsy patients and seven brain tumor patients. The control patients were children undergoing suregery for brain tumors in which measurements were taken from normal brain exposed during the surgery. Diffuse reflectance spectra were acquired for 12 seconds from various parts of the brain of the patients during surgery. Recorded spectra were processed and analyzed in both spectral and time domains to gain insights into the dynamic changes in, for example, hemodynamics of the investigated brain tissue. One finding from this pilot study is that unsynchronized alterations in local blood oxygenation and local blood volume were observed in epileptogenic cortex. These study results suggest the advantage of using a time-dependent diffuse reflectance spectroscopy system to study epileptogenic brain in vivo.

Mesoscopic structure of neuronal tracts from time-dependent diffusion

PubMed Central

Burcaw, Lauren M.; Fieremans, Els; Novikov, Dmitry S.

2015-01-01

Interpreting brain diffusion MRI measurements in terms of neuronal structure at a micrometer level is an exciting unresolved problem. Here we consider diffusion transverse to a bundle of fibers, and show theoretically, as well as using Monte Carlo simulations and measurements in a phantom made of parallel fibers mimicking axons, that the time dependent diffusion coefficient approaches its macroscopic limit slowly, in a (lnt)/t fashion. The logarithmic singularity arises due to short range disorder in the fiber packing. We identify short range disorder in axonal fibers based on histological data from the splenium, and argue that the time dependent contribution to the overall diffusion coefficient from the extra-axonal water dominates that of the intra-axonal water. This dominance may explain the bias in measuring axon diameters in clinical settings. The short range disorder is also reflected in the linear frequency dependence of the diffusion coefficient measured with oscillating gradients, in agreement with recent experiments. Our results relate the measured diffusion to the mesoscopic structure of neuronal tissue, uncovering the sensitivity of diffusion metrics to axonal arrangement within a fiber tract, and providing an alternative interpretation of axonal diameter mapping techniques. PMID:25837598

Mesoscopic structure of neuronal tracts from time-dependent diffusion.

PubMed

Burcaw, Lauren M; Fieremans, Els; Novikov, Dmitry S

2015-07-01

Interpreting brain diffusion MRI measurements in terms of neuronal structure at a micrometer level is an exciting unresolved problem. Here we consider diffusion transverse to a bundle of fibers, and show theoretically, as well as using Monte Carlo simulations and measurements in a phantom made of parallel fibers mimicking axons, that the time dependent diffusion coefficient approaches its macroscopic limit slowly, in a (ln t)/t fashion. The logarithmic singularity arises due to short range disorder in the fiber packing. We identify short range disorder in axonal fibers based on histological data from the splenium, and argue that the time dependent contribution to the overall diffusion coefficient from the extra-axonal water dominates that of the intra-axonal water. This dominance may explain the bias in measuring axon diameters in clinical settings. The short range disorder is also reflected in the asymptotically linear frequency dependence of the diffusion coefficient measured with oscillating gradients, in agreement with recent experiments. Our results relate the measured diffusion to the mesoscopic structure of neuronal tissue, uncovering the sensitivity of diffusion metrics to axonal arrangement within a fiber tract, and providing an alternative interpretation of axonal diameter mapping techniques. Copyright © 2015 Elsevier Inc. All rights reserved.

Bio-Optical Properties of the Arabian Sea as Determined by In Situ and Sea WiFS Data

NASA Technical Reports Server (NTRS)

Trees, Charles C.

1997-01-01

The overall objective of this work was to characterize optical and fluorescence properties in the euphotic zone during two British Ocean Flux Study (BOFS) Arabian Sea cruises. This was later expanded in 1995 to include three U.S. JGOFS Arabian Sea Cruises. The region was to be divided into one or more "bio-optical provinces," within each of which a single set of regression models was to be developed to relate the vertical distribution of irradiance attenuation and normalized fluorescence (SF and NF) to remote sensing reflectance and diffuse attenuation coefficient. The working hypothesis was that over relatively large spatial and temporal scales, the vertical profiles of bio-optical properties were predictable. The specific technical objectives were: (1) To characterize the vertical distribution of the inherent and apparent optical properties by measuring downwelling and upwelling irradiances, upwelling radiances, scalar irradiance of PAR, and beam transmissions at each station - from these data, spectral diffuse attenuation coefficients, irradiance reflectances, remote sensing reflectances, surface-leaving radiances and beam attenuation coefficients were determined; (2) To characterize the spectral absorption of total particulate, detrital, and dissolved organic material at each station from discrete water samples; (3) To describe the vertical distribution of photoadaptive properties in the water column by measuring profiles of stimulated (SF) and natural (NF) fluorescence and examining relationships between SF and NF as a function of diffuse optical depth, pigment biomass and primary productivity; and (4) To establish locally derived, in-water algorithms relating remote sensing reflectance spectra to diffuse attenuation coefficients, phytoplankton pigment concentrations and primary productivity, through intercomparisons with in situ measurements, for application to SeaWiFS data.

Noninvasive observation of skeletal muscle contraction using near-infrared time-resolved reflectance and diffusing-wave spectroscopy

NASA Astrophysics Data System (ADS)

Belau, Markus; Ninck, Markus; Hering, Gernot; Spinelli, Lorenzo; Contini, Davide; Torricelli, Alessandro; Gisler, Thomas

2010-09-01

We introduce a method for noninvasively measuring muscle contraction in vivo, based on near-infrared diffusing-wave spectroscopy (DWS). The method exploits the information about time-dependent shear motions within the contracting muscle that are contained in the temporal autocorrelation function g(1)(τ,t) of the multiply scattered light field measured as a function of lag time, τ, and time after stimulus, t. The analysis of g(1)(τ,t) measured on the human M. biceps brachii during repetitive electrical stimulation, using optical properties measured with time-resolved reflectance spectroscopy, shows that the tissue dynamics giving rise to the speckle fluctuations can be described by a combination of diffusion and shearing. The evolution of the tissue Cauchy strain e(t) shows a strong correlation with the force, indicating that a significant part of the shear observed with DWS is due to muscle contraction. The evolution of the DWS decay time shows quantitative differences between the M. biceps brachii and the M. gastrocnemius, suggesting that DWS allows to discriminate contraction of fast- and slow-twitch muscle fibers.

Diffuse Reflectance Spectroscopy and Colorimetry as a Diagnostic Tool for Acanthosis Nigricans

NASA Astrophysics Data System (ADS)

Pattamadilok, Bensachee; Devpura, Suneetha; Syed, Zain U.; Vemulapalli, Pranita; Henderson, Marsha; Rehse, Steven J.; Hamzavi, Iltefat; Mahmoud, Bassel H.; Lim, Henry W.; Naik, Ratna

2011-03-01

The purpose of this study was to quantify skin color changes due to Acanthosis Nigricans, a disorder common among prediabetic and obese individuals. The non-invasive optical technique diffuse reflectance spectroscopy (DRS) was used to determine skin melanin, oxyhemoglobin and deoxyhemoglobin content through the measured absorption spectrum. Colorimetery was used to measure skin color based on the standard Tristimulus values (L*, a*, and b*). Data was obtained from eight patients, spanning eight months of treatment. Measurements were obtained from lesion tissue on the neck and healthy skin was used as a control. L*, a* and b* values showed significant differences between lesion and normal controls, whereas melanin was the only parameter which showed statistical significant differences in DRS measurements. Future work will use more sensitive chemometric methods to increase diagnostic accuracy based on the raw spectra of the DRS.

Assessing human skin with diffuse reflectance spectroscopy and colorimetry

NASA Astrophysics Data System (ADS)

Seo, InSeok; Liu, Yang; Bargo, Paulo R.; Kollias, Nikiforos

2012-02-01

Colorimetry has been used as an objective measure of perceived skin color by human eye to document and score physiological responses of the skin from external insults. CIE color space values (L*, a* and b*) are the most commonly used parameters to correlate visually perceived color attributes such as L* for pigment, a* for erythema, and b* for sallowness of the skin. In this study, we investigated the relation of Lab color scale to the amount of major skin chromophores (oxy-, deoxyhemoglobin and melanin) calculated from diffuse reflectance spectroscopy. Thirty two healthy human subjects with ages from 20 to 70 years old, skin types I-VI, were recruited for the study. DRS and colorimetry measurements were taken from the left and right cheeks, and on the right upper inner arm. The melanin content calculated from 630-700 nm range of DRS measurements was shown to correlate with the lightness of skin (L*) for most skin types. For subjects with medium-to-light complexion, melanin measured at the blue part spectrum and hemoglobin interfered on the relation of lightness of the skin color to the melanin content. The sallowness of the skin that is quantified by the melanin contribution at the blue part spectrum of DRS was found to be related to b* scale. This study demonstrates the importance of documenting skin color by assessing individual skin chromophores with diffuse reflectance spectroscopy, in comparison to colorimetry assessment.

MODIS Solar Diffuser Attenuation Screen Modeling Results

NASA Technical Reports Server (NTRS)

Waluschka, Eugene; Xuong, Xiaoxiong; Guenther, Bruce; Barnes, William

2004-01-01

On-orbit calibration of the reflected solar bands on the EOS Moderate Resolution Imaging Spectroradiometer (MODIS) is accomplished by have the instrument view a high reflectance diffuse surface illuminated by the sun. For some of the spectral bands this proves to be much too bright a signal that results in the saturation of detectors designed for measuring low reflectance (ocean) surfaces signals. A mechanical attenuation device in the form of a pin hole screen is used to reduce the signals to calibrate these bands. The sensor response to solar illumination of the SD with and without the attenuation screen in place will be presented. The MODIS detector response to the solar diffuser is smooth when the attenuation screen is absent, but has structures up to a few percent when the attenuation screen is present. This structure corresponds to non-uniform illumination from the solar diffuser. Each pin hole produces a pin-hole image of the sun on the solar diffuser, and there are very many pin hole images of the sun on the solar diffuser for each MODIS detector. Even though there are very many pin-hole images of the sun on the solar diffuser, it is no longer perfectly uniformly illuminated. This non-uniformly illuminated solar diffuser produces intensity variation on the focal planes. The results of a very detailed simulation will be discussed which show how the illumination of the focal plane changes as a result of the attenuation, and the impacts on the calibration will be discussed.

BRDF Calibration of Sintered PTFE in the SWIR

NASA Technical Reports Server (NTRS)

Georgiev, Georgi T.; Butler, James J.

2009-01-01

Satellite instruments operating in the reflective solar wavelength region often require accurate and precise determination of the Bidirectional Reflectance Distribution Function (BRDF) of laboratory-based diffusers used in their pre-flight calibrations and ground-based support of on-orbit remote sensing instruments. The Diffuser Calibration Facility at NASA's Goddard Space Flight Center is a secondary diffuser calibration standard after NEST for over two decades, providing numerous NASA projects with BRDF data in the UV, Visible and the NIR spectral regions. Currently the Diffuser Calibration Facility extended the covered spectral range from 900 nm up to 1.7 microns. The measurements were made using the existing scatterometer by replacing the Si photodiode based receiver with an InGaAs-based one. The BRDF data was recorded at normal incidence and scatter zenith angles from 10 to 60 deg. Tunable coherent light source was setup. Broadband light source application is under development. Gray-scale sintered PTFE samples were used at these first trials, illuminated with P and S polarized incident light. The results are discussed and compared to empirically generated BRDF data from simple model based on 8 deg directional/hemispherical measurements.

Application of the three-component bidirectional reflectance distribution function model to Monte Carlo calculation of spectral effective emissivities of nonisothermal blackbody cavities.

PubMed

Prokhorov, Alexander; Prokhorova, Nina I

2012-11-20

We applied the bidirectional reflectance distribution function (BRDF) model consisting of diffuse, quasi-specular, and glossy components to the Monte Carlo modeling of spectral effective emissivities for nonisothermal cavities. A method for extension of a monochromatic three-component (3C) BRDF model to a continuous spectral range is proposed. The initial data for this method are the BRDFs measured in the plane of incidence at a single wavelength and several incidence angles and directional-hemispherical reflectance measured at one incidence angle within a finite spectral range. We proposed the Monte Carlo algorithm for calculation of spectral effective emissivities for nonisothermal cavities whose internal surface is described by the wavelength-dependent 3C BRDF model. The results obtained for a cylindroconical nonisothermal cavity are discussed and compared with results obtained using the conventional specular-diffuse model.

Influence of earlobe thickness on near infrared spectroscopy

NASA Astrophysics Data System (ADS)

Jiang, Jingying; Wang, Tianpei; Li, Si; Li, Lin; Liu, Jiajia; Xu, Kexin

2017-03-01

Near-infrared spectroscopy has been recognized as a potential technology for noninvasive blood glucose sensing. However, the detected spectral signal is unstable mainly because of (1) the weak light absorption of glucose itself within NIR range, (2) the influence of temperature and individual differences of biotissue. Our previous results demonstrated that the synergistic effect of both transmittance and reflectance could enhance the strength of the detection signal. In this talk, we design a set of experiments to analyze the effect of earlobe thickness on Near Infrared spectroscopic measurement by using home-made optical fiber probe within the wavelength of 1000-1600nm. Firstly, we made a MC simulation of single-layer skin model and five-layer skin model to get the diffused transmittance spectra and diffused reflectance spectra under different optaical path lengths. And then we obtain the spectra of the earlobes from different volunteers by the same way. The experimental results showed that with the increase of the thickness,the light intensity of diffused transmittance decreases, and the light intensity of diffused reflectance remaines substantially unchanged.

The effect of soil water deficit on the reflectance of conifer seedling canopies

NASA Technical Reports Server (NTRS)

Fox, L.

1977-01-01

The effects of soil water deficit on spruce and pine seedling canopy reflectance, needle reflectance and transmittance, and canopy density were measured in a greenhouse with a diffuse source of radiant flux. A potential for early or pre-visual detection of plant water stress was not supported by these measurements made at visible, and reflected infrared wavelengths to 1950 nm. Needles were found to transmit approximately thirty percent of the radiant flux incident on them at 780 nm, ten percent at 700 nm, and were found to be opaque at 450, 550, 600 and 650 nm.

Characterizing Intraorbital Optic Nerve Changes on Diffusion Tensor Imaging in Thyroid Eye Disease Before Dysthyroid Optic Neuropathy.

PubMed

Lee, Hwa; Lee, Young Hen; Suh, Sang-Il; Jeong, Eun-Kee; Baek, Sehyun; Seo, Hyung Suk

The aim of this study was to determine whether the optic nerve is affected by thyroid eye disease (TED) before the development of dysthyroid optic neuropathy with diffusion-tensor imaging (DTI). Twenty TED patients and 20 controls were included. The mean, axial, and radial diffusivities and fractional anisotropy (FA) value were measured at the optic nerves in DTI. Extraocular muscle diameters were measured on computed tomography. The diffusivities and FA of the optic nerves were compared between TED and controls and between active and inactive stages of TED. The correlations between these DTI parameters and the clinical features were determined. The mean, axial, and radial diffusivities were lower in TED compared with the controls (P < 0.05). In contrast, FA was higher in TED (P = 0.001). Radial diffusivity was lower in the active stage of TED than the inactive stage (P = 0.035). The FA was higher in the TED group than in the control group (P = 0.021) and was positively correlated with clinical activity score (r = 0.364, P = 0.021), modified NOSPECS score (r = 0.469, P = 0.002), and extraocular muscle thickness (r = 0.325, P = 0.041) in the TED group. Radial diffusivity was negatively correlated with modified NOSPECS score (r = -0.384, P = 0.014), and axial diffusivity was positively correlated with exophthalmos degree (r = 0.363, P = 0.025). The diffusivities and FA reflected changes in the optic nerve before dysthyroid optic neuropathy in TED. The FA, in particular, reflected TED activity and severity.

An improved method to estimate reflectance parameters for high dynamic range imaging

NASA Astrophysics Data System (ADS)

Li, Shiying; Deguchi, Koichiro; Li, Renfa; Manabe, Yoshitsugu; Chihara, Kunihiro

2008-01-01

Two methods are described to accurately estimate diffuse and specular reflectance parameters for colors, gloss intensity and surface roughness, over the dynamic range of the camera used to capture input images. Neither method needs to segment color areas on an image, or to reconstruct a high dynamic range (HDR) image. The second method improves on the first, bypassing the requirement for specific separation of diffuse and specular reflection components. For the latter method, diffuse and specular reflectance parameters are estimated separately, using the least squares method. Reflection values are initially assumed to be diffuse-only reflection components, and are subjected to the least squares method to estimate diffuse reflectance parameters. Specular reflection components, obtained by subtracting the computed diffuse reflection components from reflection values, are then subjected to a logarithmically transformed equation of the Torrance-Sparrow reflection model, and specular reflectance parameters for gloss intensity and surface roughness are finally estimated using the least squares method. Experiments were carried out using both methods, with simulation data at different saturation levels, generated according to the Lambert and Torrance-Sparrow reflection models, and the second method, with spectral images captured by an imaging spectrograph and a moving light source. Our results show that the second method can estimate the diffuse and specular reflectance parameters for colors, gloss intensity and surface roughness more accurately and faster than the first one, so that colors and gloss can be reproduced more efficiently for HDR imaging.

Numerical Modelling of the Sound Fields in Urban Streets with Diffusely Reflecting Boundaries

NASA Astrophysics Data System (ADS)

KANG, J.

2002-12-01

A radiosity-based theoretical/computer model has been developed to study the fundamental characteristics of the sound fields in urban streets resulting from diffusely reflecting boundaries, and to investigate the effectiveness of architectural changes and urban design options on noise reduction. Comparison between the theoretical prediction and the measurement in a scale model of an urban street shows very good agreement. Computations using the model in hypothetical rectangular streets demonstrate that though the boundaries are diffusely reflective, the sound attenuation along the length is significant, typically at 20-30 dB/100 m. The sound distribution in a cross-section is generally even unless the cross-section is very close to the source. In terms of the effectiveness of architectural changes and urban design options, it has been shown that over 2-4 dB extra attenuation can be obtained either by increasing boundary absorption evenly or by adding absorbent patches on the façades or the ground. Reducing building height has a similar effect. A gap between buildings can provide about 2-3 dB extra sound attenuation, especially in the vicinity of the gap. The effectiveness of air absorption on increasing sound attenuation along the length could be 3-9 dB at high frequencies. If a treatment is effective with a single source, it is also effective with multiple sources. In addition, it has been demonstrated that if the façades in a street are diffusely reflective, the sound field of the street does not change significantly whether the ground is diffusely or geometrically reflective.

Development of a wearable CMOS-based contact imaging system for real-time skin condition diagnosis

NASA Astrophysics Data System (ADS)

Petitdidier, Nils; Koenig, Anne; Gerbelot, Rémi; Gioux, Sylvain; Dinten, Jean-Marc

2017-07-01

Diffuse reflectance spectroscopy has been widely used in the field of biological tissue characterization with various modalities [1-5,6]. One of these modalities consists in measuring the spatially resolved diffuse reflectance (SRDR). In this technique, light is collected at multiple distances from the excitation point. The obtained reflectance decay curve is used to determine scattering and absorption properties of the tissue [7], which are directly related to tissue content and structure. Existing systems usually use fiber optics to collect light reflected from the tissue and transfer it to an optical sensor [1,6]. Such devices make it possible to perform SRDR measurements directly in contact with the tissue. However, they offer poor spatial sampling of the reflectance and low light collection efficiency. We propose to overcome these limitations by using a CMOS sensor placed in contact with the tissue to achieve light collection with high spatial sampling over several millimeters and with increased fill factor. Our objective in this paper is to demonstrate the potential of our instrument to determine the optical properties of tissues from SRDR measurements. We first describe the instrument and the employed methodology. Then, preliminary results obtained on optical phantoms are presented. Finally, the potential of our system for SRDR measurements is evaluated through comparison with a fiber-optic probe previously developed in our laboratory [6,8].

Implementation of cost-effective diffuse light source mechanism to reduce specular reflection and halo effects for resistor-image processing

NASA Astrophysics Data System (ADS)

Chen, Yung-Sheng; Wang, Jeng-Yau

2015-09-01

Light source plays a significant role to acquire a qualified image from objects for facilitating the image processing and pattern recognition. For objects possessing specular surface, the phenomena of reflection and halo appearing in the acquired image will increase the difficulty of information processing. Such a situation may be improved by the assistance of valuable diffuse light source. Consider reading resistor via computer vision, due to the resistor's specular reflective surface it will face with a severe non-uniform luminous intensity on image yielding a higher error rate in recognition without a well-controlled light source. A measurement system including mainly a digital microscope embedded in a replaceable diffuse cover, a ring-type LED embedded onto a small pad carrying a resistor for evaluation, and Arduino microcontrollers connected with PC, is presented in this paper. Several replaceable cost-effective diffuse covers made by paper bowl, cup and box inside pasted with white paper are presented for reducing specular reflection and halo effects and compared with a commercial diffuse some. The ring-type LED can be flexibly configured to be a full or partial lighting based on the application. For each self-made diffuse cover, a set of resistors with 4 or 5 color bands are captured via digital microscope for experiments. The signal-to-noise ratio from the segmented resistor-image is used for performance evaluation. The detected principal axis of resistor body is used for the partial LED configuration to further improve the lighting condition. Experimental results confirm that the proposed mechanism can not only evaluate the cost-effective diffuse light source but also be extended as an automatic recognition system for resistor reading.

Softwall acoustical characteristics and measurement capabilities of the NASA Lewis 9x15 foot low speed wind tunnel

NASA Technical Reports Server (NTRS)

Rentz, P. E.

1976-01-01

Acoustical characteristics and source directionality measurement capabilities of the wind tunnel in the softwall configuration were evaluated, using aerodynamically clean microphone supports. The radius of measurement was limited by the size of the test section, instead of the 3.0 foot (1 m) limitation of the hardwall test section. The wind-on noise level in the test section was reduced 10 dB. Reflections from the microphone support boom, after absorptive covering, induced measurement errors in the lower frequency bands. Reflections from the diffuser back wall were shown to be significant. Tunnel noise coming up the diffuser was postulated as being responsible, at least partially, for the wind-on noise in the test section and settling chamber. The near field characteristics of finite-sized sources and the theoretical response of a porous strip sensor in the presence of wind are presented.

A novel image-based BRDF measurement system and its application to human skin

NASA Astrophysics Data System (ADS)

Bintz, Jeffrey R.; Mendenhall, Michael J.; Marciniak, Michael A.; Butler, Samuel D.; Lloyd, James Tommy

2016-09-01

Human skin detection is an important first step in search and rescue (SAR) scenarios. Previous research performed human skin detection through an application specific camera system that ex- ploits the spectral properties of human skin at two visible and two near-infrared (NIR) wavelengths. The current theory assumes human skin is diffuse; however, it is observed that human skin exhibits specular and diffuse reflectance properties. This paper presents a novel image-based bidirectional reflectance distribution function (BRDF) measurement system, and applies it to the collection of human skin BRDF. The system uses a grid projecting laser and a novel signal processing chain to extract the surface normal from each grid location. Human skin BRDF measurements are shown for a variety of melanin content and hair coverage at the four spectral channels needed for human skin detection. The NIR results represent a novel contribution to the existing body of human skin BRDF measurements.

Re-evaluation of model-based light-scattering spectroscopy for tissue spectroscopy

PubMed Central

Lau, Condon; Šćepanović, Obrad; Mirkovic, Jelena; McGee, Sasha; Yu, Chung-Chieh; Fulghum, Stephen; Wallace, Michael; Tunnell, James; Bechtel, Kate; Feld, Michael

2009-01-01

Model-based light scattering spectroscopy (LSS) seemed a promising technique for in-vivo diagnosis of dysplasia in multiple organs. In the studies, the residual spectrum, the difference between the observed and modeled diffuse reflectance spectra, was attributed to single elastic light scattering from epithelial nuclei, and diagnostic information due to nuclear changes was extracted from it. We show that this picture is incorrect. The actual single scattering signal arising from epithelial nuclei is much smaller than the previously computed residual spectrum, and does not have the wavelength dependence characteristic of Mie scattering. Rather, the residual spectrum largely arises from assuming a uniform hemoglobin distribution. In fact, hemoglobin is packaged in blood vessels, which alters the reflectance. When we include vessel packaging, which accounts for an inhomogeneous hemoglobin distribution, in the diffuse reflectance model, the reflectance is modeled more accurately, greatly reducing the amplitude of the residual spectrum. These findings are verified via numerical estimates based on light propagation and Mie theory, tissue phantom experiments, and analysis of published data measured from Barrett’s esophagus. In future studies, vessel packaging should be included in the model of diffuse reflectance and use of model-based LSS should be discontinued. PMID:19405760

A novel grating-imaging method to measure carrier diffusion coefficient in graphene

NASA Astrophysics Data System (ADS)

Chen, Ke; Wang, Yaguo; Akinwande, Deji; Bank, Seth; Lin, Jung-Fu

Similar to carrier mobility, carrier diffusion coefficient in graphene determines the response rate of future graphene-based electronics. Here we present a simple, sensitive and non-destructive technique integrated with ultrafast pump-probe spectroscopy to measure carrier diffusion in CVD-grown graphene. In the method, the pump and the probe beams pass through the same area of a photomask with metal strips i.e. a transmission amplitude grating, and get diffracted. The diffracted light is collected by an objective lens and focused onto the sample to generate carrier density grating. Relaxation of this carrier density grating is governed by both carrier recombination and carrier diffusion in the sample. Transient transmission change of the probe beams, which reflects this relaxation process, is recorded. The measured diffusion coefficients of multilayer and monolayer CVD-grown graphene are 2000cm2/s and 10000cm2/s, respectively, comparable with the reported values of epitaxial graphene and reduced graphene. This transmission grating technique can be used to measure carrier dynamics in versatile 2D materials.

High reflected cubic cavity as long path absorption cell for infrared gas sensing

NASA Astrophysics Data System (ADS)

Yu, Jia; Gao, Qiang; Zhang, Zhiguo

2014-10-01

One direct and efficient method to improve the sensitivity of infrared gas sensors is to increase the optical path length of gas cells according to Beer-Lambert Law. In this paper, cubic shaped cavities with high reflected inner coating as novel long path absorption cells for infrared gas sensing were developed. The effective optical path length (EOPL) for a single cubic cavity and tandem cubic cavities were investigated based on Tunable Diode Laser Absorption Spectroscopy (TDLAS) measuring oxygen P11 line at 763 nm. The law of EOPL of a diffuse cubic cavity in relation with the reflectivity of the coating, the port fraction and side length of the cavity was obtained. Experimental results manifested an increase of EOPL for tandem diffuse cubic cavities as the decrease of port fraction of the connecting aperture f', and the EOPL equaled to the sum of that of two single cubic cavities at f'<0.01. The EOPL spectra at infrared wavelength range for different inner coatings including high diffuse coatings and high reflected metallic thin film coatings were deduced.

Spectralon BRDF and DHR Measurements in Support of Satellite Instruments Operating Through Shortwave Infrared

NASA Technical Reports Server (NTRS)

Georgiev, Georgi T.; Butler, James J.; Thome, Kurt; Cooksey, Catherine; Ding, Leibo

2016-01-01

Satellite instruments operating in the reflective solar wavelength region require accurate and precise determination of the Bidirectional Reflectance Distribution Functions (BRDFs) of the laboratory and flight diffusers used in their pre-flight and on-orbit calibrations. This paper advances that initial work and presents a comparison of spectral Bidirectional Reflectance Distribution Function (BRDF) and Directional Hemispherical Reflectance (DHR) of Spectralon*, a common material for laboratory and onorbit flight diffusers. A new measurement setup for BRDF measurements from 900 nm to 2500 nm located at NASA Goddard Space Flight Center (GSFC) is described. The GSFC setup employs an extended indium gallium arsenide detector, bandpass filters, and a supercontinuum light source. Comparisons of the GSFC BRDF measurements in the ShortWave InfraRed (SWIR) with those made by the NIST Spectral Trifunction Automated Reference Reflectometer (STARR) are presented. The Spectralon sample used in this study was 2 inch diameter, 99% white pressed and sintered Polytetrafluoroethylene (PTFE) target. The NASA/NIST BRDF comparison measurements were made at an incident angle of 0 deg and viewing angle of 45 deg. Additional BRDF data not compared to NIST were measured at additional incident and viewing angle geometries and are not presented here The total combined uncertainty for the measurement of BRDF in the SWIR range made by the GSFC scatterometer is less than 1% (k=1). This study is in support of the calibration of the Joint Polar Satellite System (JPSS) Radiation Budget Instrument (RBI) and Visible Infrared Imaging Radiometer Suite (VIIRS) of and other current and future NASA remote sensing missions operating across the reflected solar wavelength region.

Time-domain reflectance diffuse optical tomography with Mellin-Laplace transform for experimental detection and depth localization of a single absorbing inclusion

PubMed Central

Puszka, Agathe; Hervé, Lionel; Planat-Chrétien, Anne; Koenig, Anne; Derouard, Jacques; Dinten, Jean-Marc

2013-01-01

We show how to apply the Mellin-Laplace transform to process time-resolved reflectance measurements for diffuse optical tomography. We illustrate this method on simulated signals incorporating the main sources of experimental noise and suggest how to fine-tune the method in order to detect the deepest absorbing inclusions and optimize their localization in depth, depending on the dynamic range of the measurement. To finish, we apply this method to measurements acquired with a setup including a femtosecond laser, photomultipliers and a time-correlated single photon counting board. Simulations and experiments are illustrated for a probe featuring the interfiber distance of 1.5 cm and show the potential of time-resolved techniques for imaging absorption contrast in depth with this geometry. PMID:23577292

Characterizing the audibility of sound field with diffusion in architectural spaces

NASA Astrophysics Data System (ADS)

Utami, Sentagi Sesotya

The significance of diffusion control in room acoustics is that it attempts to avoid echoes by dispersing reflections while removing less valuable sound energy. Some applications place emphasis on the enhancement of late reflections to promote a sense of envelopment, and on methods required to measure the performance of diffusers. What still remains unclear is the impact of diffusion on the audibility quality due to the geometric arrangement of architectural elements. The objective of this research is to characterize the audibility of the sound field with diffusion in architectural space. In order to address this objective, an approach utilizing various methods and new techniques relevant to room acoustics standards was applied. An array of microphones based on beam forming (i.e., an acoustic camera) was utilized for field measurements in a recording studio, classrooms, auditoriums, concert halls and sport arenas. Given the ability to combine a visual image with acoustical data, the impulse responses measured were analyzed to identify the impact of diffusive surfaces on the early, late, and reverberant sound fields. The effects of the room geometry and the proportions of the diffusive and absorptive surfaces were observed by utilizing geometrical room acoustics simulations. The degree of diffuseness in each space was measured by coherences from different measurement positions along with the acoustical conditions predicted by well-known objective parameters such as T30, EDT, C80, and C50. Noticeable differences of the auditory experience were investigated by utilizing computer-based survey techniques, including the use of an immersive virtual environment system, given the current software auralization capabilities. The results based on statistical analysis demonstrate the users' ability to localize the sound and to distinguish the intensity, clarity, and reverberation created within the virtual environment. Impact of architectural elements in diffusion control is evaluated by the design variable interaction, objectively and subjectively. Effectiveness of the diffusive surfaces is determined by the echo reduction and the sense of complete immersion in a given room acoustics volume. Application of such methodology at various stages of design provides the ability to create a better auditory experience by the users. The results based on the cases studied have contributed to the development of new acoustical treatment based on the diffusion characteristics.

Angular distribution of diffuse reflectance from incoherent multiple scattering in turbid media.

PubMed

Gao, M; Huang, X; Yang, P; Kattawar, G W

2013-08-20

The angular distribution of diffuse reflection is elucidated with greater understanding by studying a homogeneous turbid medium. We modeled the medium as an infinite slab and studied the reflection dependence on the following three parameters: the incident direction, optical depth, and asymmetry factor. The diffuse reflection is produced by incoherent multiple scattering and is solved through radiative transfer theory. At large optical depths, the angular distribution of the diffuse reflection with small incident angles is similar to that of a Lambertian surface, but, with incident angles larger than 60°, the angular distributions have a prominent reflection peak around the specular reflection angle. These reflection peaks are found originating from the scattering within one transport mean free path in the top layer of the medium. The maximum reflection angles for different incident angles are analyzed and can characterize the structure of angular distributions for different asymmetry factors and optical depths. The properties of the angular distribution can be applied to more complex systems for a better understanding of diffuse reflection.

Why do veins appear blue? A new look at an old question

NASA Astrophysics Data System (ADS)

Kienle, Alwin; Hibst, Raimund; Steiner, Rudolf; Lilge, Lothar; Vitkin, I. Alex; Wilson, Brian C.; Patterson, Michael S.

1996-03-01

We investigate why vessels that contain blood, which has a red or a dark red color, may look bluish in human tissue. A CCD camera was used to make images of diffusely reflected light at different wavelengths. Measurements of reflectance that are due to model blood vessels in scattering media and of human skin containing a prominent vein are presented. Monte Carlo simulations were used to calculate the spatially resolved diffuse reflectance for both situations. We show that the color of blood vessels is scattering and absorption characteristics of skin at different wavelengths, (ii) the oxygenation state of blood, which affects its absorption properties, (iii) the diameter and the depth of the vessels, and (iv) the visual perception process.

Jigsaw puzzle metasurface for multiple functions: polarization conversion, anomalous reflection and diffusion.

PubMed

Zhao, Yi; Cao, Xiangyu; Gao, Jun; Liu, Xiao; Li, Sijia

2016-05-16

We demonstrate a simple reconfigurable metasurface with multiple functions. Anisotropic tiles are investigated and manufactured as fundamental elements. Then, the tiles are combined in a certain sequence to construct a metasurface. Each of the tiles can be adjusted independently which is like a jigsaw puzzle and the whole metasurface can achieve diverse functions by different layouts. For demonstration purposes, we realize polarization conversion, anomalous reflection and diffusion by a jigsaw puzzle metasurface with 6 × 6 pieces of anisotropic tile. Simulated and measured results prove that our method offers a simple and effective strategy for metasurface design.

A simple and green analytical method for determination of glyphosate in commercial formulations and water by diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

da Silva, Aline Santana; Fernandes, Flávio Cesar Bedatty; Tognolli, João Olímpio; Pezza, Leonardo; Pezza, Helena Redigolo

2011-09-01

This article describes a simple, inexpensive, and environmentally friendly method for the monitoring of glyphosate using diffuse reflectance spectroscopy. The proposed method is based on reflectance measurements of the colored compound produced from the spot test reaction between glyphosate and p-dimethylaminocinnamaldehyde ( p-DAC) in acid medium, using a filter paper as solid support. Experimental designs were used to optimize the analytical conditions. All reflectance measurements were carried out at 495 nm. Under optimal conditions, the glyphosate calibration graphs obtained by plotting the optical density of the reflectance signal (A R) against the concentration were linear in the range 50-500 μg mL -1, with a correlation coefficient of 0.9987. The limit of detection (LOD) for glyphosate was 7.28 μg mL -1. The technique was successfully applied to the direct determination of glyphosate in commercial formulations, as well as in water samples (river water, pure water and mineral drinking water) after a previous clean-up or pre-concentration step. Recoveries were in the ranges 93.2-102.6% and 91.3-102.9% for the commercial formulations and water samples, respectively.

A simple and green analytical method for determination of glyphosate in commercial formulations and water by diffuse reflectance spectroscopy.

PubMed

da Silva, Aline Santana; Fernandes, Flávio Cesar Bedatty; Tognolli, João Olímpio; Pezza, Leonardo; Pezza, Helena Redigolo

2011-09-01

This article describes a simple, inexpensive, and environmentally friendly method for the monitoring of glyphosate using diffuse reflectance spectroscopy. The proposed method is based on reflectance measurements of the colored compound produced from the spot test reaction between glyphosate and p-dimethylaminocinnamaldehyde (p-DAC) in acid medium, using a filter paper as solid support. Experimental designs were used to optimize the analytical conditions. All reflectance measurements were carried out at 495 nm. Under optimal conditions, the glyphosate calibration graphs obtained by plotting the optical density of the reflectance signal (AR) against the concentration were linear in the range 50-500 μg mL(-1), with a correlation coefficient of 0.9987. The limit of detection (LOD) for glyphosate was 7.28 μg mL(-1). The technique was successfully applied to the direct determination of glyphosate in commercial formulations, as well as in water samples (river water, pure water and mineral drinking water) after a previous clean-up or pre-concentration step. Recoveries were in the ranges 93.2-102.6% and 91.3-102.9% for the commercial formulations and water samples, respectively. Copyright © 2011 Elsevier B.V. All rights reserved.

Expression of the degree of polarization based on the geometrical optics pBRDF model.

PubMed

Wang, Kai; Zhu, Jingping; Liu, Hong; Du, Bingzheng

2017-02-01

An expression of the degree of polarization (DOP) based on the geometrical optics polarimetric bidirectional reflectance distribution function model is presented. In this expression, the DOP is related to the surface roughness and decreases at different reflection angles because diffuse reflection is taken into consideration. A shadowing/masking function introduced into the specular reflection expression makes the DOP values decrease as the angle of incidence or observation approaches grazing. Different kinds of materials were measured to validate the accuracy of this DOP expression. The measured results suggest that the errors of the DOP are reduced significantly, and the polarized reflection characteristics can be described more reasonably and accurately.

Diffuse reflectance spectroscopy from 400-1600 nm to evaluate tumor resection margins during head and neck surgery (Conference Presentation)

NASA Astrophysics Data System (ADS)

Brouwer de Koning, Susan G.; Baltussen, E. J. M.; Karakullukcu, M. Baris; Smit, L.; van Veen, R. L. P.; Hendriks, Benno H. W.; Sterenborg, H. J. C. M.; Ruers, Theo J. M.

2017-02-01

This ex vivo study evaluates the feasibility of diffuse reflectance spectroscopy (DRS) for discriminating tumor from healthy oral tissue, with the aim to develop a technique that can be used to determine a complete excision of tumor through intraoperative margin assessment. DRS spectra were acquired on fresh surgical specimens from patients with an oral squamous cell carcinoma. The spectra represent a measure of diffuse light reflectance (wavelength range of 400-1600 nm), detected after illuminating tissue with a source fiber at 1.0 and 2.0 mm distances from a detection fiber. Spectra were obtained from 23 locations of tumor tissue and 16 locations of healthy muscle tissue. Biopsies were taken from all measured locations to facilitate an optimal correlation between spectra and pathological information. The area under the spectrum was used as a parameter to classify spectra of tumor and healthy tissue. Next, a receiver operating characteristics (ROC) analysis was performed to provide the area under the receiver operating curve (AUROC) as a measure for discriminative power. The area under the spectrum between 650 and 750 nm was used in the ROC analysis and provided AUROC values of 0.99 and 0.97, for distances of 1 mm and 2 mm between source and detector fiber, respectively. DRS can discriminate tumor from healthy oral tissue in an ex vivo setting. More specimens are needed to further evaluate this technique with component analyses and classification methods, prior to in vivo patient measurements.

Quantitative characterization of turbidity by radiative transfer based reflectance imaging

PubMed Central

Tian, Peng; Chen, Cheng; Jin, Jiahong; Hong, Heng; Lu, Jun Q.; Hu, Xin-Hua

2018-01-01

A new and noncontact approach of multispectral reflectance imaging has been developed to inversely determine the absorption coefficient of μa, the scattering coefficient of μs and the anisotropy factor g of a turbid target from one measured reflectance image. The incident beam was profiled with a diffuse reflectance standard for deriving both measured and calculated reflectance images. A GPU implemented Monte Carlo code was developed to determine the parameters with a conjugate gradient descent algorithm and the existence of unique solutions was shown. We noninvasively determined embedded region thickness in heterogeneous targets and estimated in vivo optical parameters of nevi from 4 patients between 500 and 950nm for melanoma diagnosis to demonstrate the potentials of quantitative reflectance imaging. PMID:29760971

Establishing BRDF calibration capabilities through shortwave infrared

NASA Astrophysics Data System (ADS)

Georgiev, Georgi T.; Butler, James J.; Thome, Kurt; Cooksey, Catherine; Ding, Leibo

2017-09-01

Satellite instruments operating in the reflective solar wavelength region require accurate and precise determination of the Bidirectional Reflectance Distribution Functions (BRDFs) of the laboratory and flight diffusers used in their pre-flight and on-orbit calibrations. This paper advances that initial work and presents a comparison of spectral Bidirectional Reflectance Distribution Function (BRDF) and Directional Hemispherical Reflectance (DHR) of Spectralon*, a common material for laboratory and onorbit flight diffusers. A new measurement setup for BRDF measurements from 900 nm to 2500 nm located at NASA Goddard Space Flight Center (GSFC) is described. The GSFC setup employs an extended indium gallium arsenide detector, bandpass filters, and a supercontinuum light source. Comparisons of the GSFC BRDF measurements in the shortwave infrared (SWIR) with those made by the National Institute of Standards and Technology (NIST) Spectral Tri-function Automated Reference Reflectometer (STARR) are presented. The Spectralon sample used in this study was 2 inch diameter, 99% white pressed and sintered Polytetrafluoroethylene (PTFE) target. The NASA/NIST BRDF comparison measurements were made at an incident angle of 0° and viewing angle of 45° . Additional BRDF data not compared to NIST were measured at additional incident and viewing angle geometries and are not presented here. The total combined uncertainty for the measurement of BRDF in the SWIR range made by the GSFC scatterometer is less than 1% (k = 1). This study is in support of the calibration of the Radiation Budget Instrument (RBI) and Visible Infrared Imaging Radiometer Suit (VIIRS) instruments of the Joint Polar Satellite System (JPSS) and other current and future NASA remote sensing missions operating across the reflected solar wavelength region.

ESTABLISHING BRDF CALIBRATION CAPABILITIES THROUGH SHORTWAVE INFRARED.

PubMed

Georgiev, Georgi T; Butler, James J; Thome, Kurt; Cooksey, Catherine; Ding, Leibo

2017-01-01

Satellite instruments operating in the reflective solar wavelength region require accurate and precise determination of the Bidirectional Reflectance Distribution Functions (BRDFs) of the laboratory and flight diffusers used in their pre-flight and on-orbit calibrations. This paper advances that initial work and presents a comparison of spectral Bidirectional Reflectance Distribution Function (BRDF) and Directional Hemispherical Reflectance (DHR) of Spectralon, a common material for laboratory and on-orbit flight diffusers. A new measurement setup for BRDF measurements from 900 nm to 2500 nm located at NASA Goddard Space Flight Center (GSFC) is described. The GSFC setup employs an extended indium gallium arsenide detector, bandpass filters, and a supercontinuum light source. Comparisons of the GSFC BRDF measurements in the shortwave infrared (SWIR) with those made by the National Institute of Standards and Technology (NIST) Spectral Tri-function Automated Reference Reflectometer (STARR) are presented. The Spectralon sample used in this study was 2 inch diameter, 99% white pressed and sintered Polytetrafluoroethylene (PTFE) target. The NASA/NIST BRDF comparison measurements were made at an incident angle of 0° and viewing angle of 45°. Additional BRDF data not compared to NIST were measured at additional incident and viewing angle geometries and are not presented here. The total combined uncertainty for the measurement of BRDF in the SWIR range made by the GSFC scatterometer is less than 1% ( k = 1). This study is in support of the calibration of the Radiation Budget Instrument (RBI) and Visible Infrared Imaging Radiometer Suit (VIIRS) instruments of the Joint Polar Satellite System (JPSS) and other current and future NASA remote sensing missions operating across the reflected solar wavelength region.

Frequency-domain optical absorption spectroscopy of finite tissue volumes using diffusion theory.

PubMed

Pogue, B W; Patterson, M S

1994-07-01

The goal of frequency-domain optical absorption spectroscopy is the non-invasive determination of the absorption coefficient of a specific tissue volume. Since this allows the concentration of endogenous and exogenous chromophores to be calculated, there is considerable potential for clinical application. The technique relies on the measurement of the phase and modulation of light, which is diffusely reflected or transmitted by the tissue when it is illuminated by an intensity-modulated source. A model of light propagation must then be used to deduce the absorption coefficient. For simplicity, it is usual to assume the tissue is either infinite in extent (for transmission measurements) or semi-infinite (for reflectance measurements). The goal of this paper is to examine the errors introduced by these assumptions when measurements are actually performed on finite volumes. Diffusion-theory calculations and experimental measurements were performed for slabs, cylinders and spheres with optical properties characteristic of soft tissues in the near infrared. The error in absorption coefficient is presented as a function of object size as a guideline to when the simple models may be used. For transmission measurements, the error is almost independent of the true absorption coefficient, which allows absolute changes in absorption to be measured accurately. The implications of these errors in absorption coefficient for two clinical problems--quantitation of an exogenous photosensitizer and measurement of haemoglobin oxygenation--are presented and discussed.

Diffusion weighted imaging reflects variable cellularity and stromal density present in breast fibroadenomas

PubMed Central

Parsian, Sana; Giannakopoulos, Nadia V.; Rahbar, Habib; Rendi, Mara H.; Chai, Xiaoyu

2016-01-01

OBJECTIVE To determine the underlying histopathologic features influencing apparent diffusion coefficient (ADC) values of breast fibroadenomas. MATERIALS AND METHODS Biopsy proven fibroadenomas (n=26) initially identified as suspicious on breast MRI were retrospectively evaluated. Histopathological assessments of lesion cellularity and stromal type were compared with ADC measures on diffusion-weighted MRI. RESULTS Presence of epithelial hyperplasia (increased cellularity) and dense collagenous stroma were both significantly associated with lower lesion ADC values (p=0.02 and 0.004, respectively. CONCLUSION Variations in epithelial cellularity and stromal type influence breast lesion ADC values and may explain the wide range of ADC measures observed in benign fibroadenomas. PMID:27379441

Generalizing the flash technique in the front-face configuration to measure the thermal diffusivity of semitransparent solids

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pech-May, Nelson Wilbur; Department of Applied Physics, CINVESTAV Unidad Mérida, carretera Antigua a Progreso km6, A.P. 73 Cordemex, Mérida Yucatán 97310, México; Mendioroz, Arantza

2014-10-15

In this work, we have extended the front-face flash method to retrieve simultaneously the thermal diffusivity and the optical absorption coefficient of semitransparent plates. A complete theoretical model that allows calculating the front surface temperature rise of the sample has been developed. It takes into consideration additional effects, such as multiple reflections of the heating light beam inside the sample, heat losses by convection and radiation, transparency of the sample to infrared wavelengths, and heating pulse duration. Measurements performed on calibrated solids, covering a wide range of absorption coefficients (from transparent to opaque) and thermal diffusivities, validate the proposed method.

Vertical-type chiroptical spectrophotometer (I): instrumentation and application to diffuse reflectance circular dichroism measurement.

PubMed

Harada, Takunori; Hayakawa, Hiroshi; Kuroda, Reiko

2008-07-01

We have designed and built a novel universal chiroptical spectrophotometer (UCS-2: J-800KCMF), which can carry out in situ chirality measurement of solid samples without any pretreatment, in the UV-vis region and with high relative efficiency. The instrument was designed to carry out transmittance and diffuse reflectance (DR) circular dichroism (CD) measurements simultaneously, thus housing two photomultipliers. It has a unique feature that light impinges on samples vertically so that loose powders can be measured by placing them on a flat sample holder in an integrating sphere. As is our first universal chiroptical spectrophotometer, UCS-1, two lock-in amplifiers are installed to remove artifact signals arising from macroscopic anisotropies which are unique to solid samples. High performance was achieved by theoretically analyzing and experimentally proven the effect of the photoelastic modulator position on the CD base line shifts, and by selecting high-quality optical and electric components. Measurement of microcrystallines of both enantiomers of ammonium camphorsulfonate by the DRCD mode gave reasonable results.

Final report on the key comparison CCPR-K5: Spectral diffuse reflectance

NASA Astrophysics Data System (ADS)

Nadal, Maria; Eckerle, Kenneth L.; Early, Edward A.; Ohno, Yoshi

2013-01-01

The CCPR K5 key comparison on spectral diffuse reflectance was carried out in the framework of the CIPM Mutual Recognition Arrangement, by 13 national metrology institutes (MMIs) as participants. The participants were CSIR-NML (South Africa), HUT (Finland), IFA-CSIC (Spain), KRISS (Republic of Korea), MSL (New Zealand), NIM (China), NIST (United States of America), NMIJ (Japan), NPL (United Kingdom), NRC (Canada), OMH (Hungary), PTB (Germany) and VNIIOFI (Russia Federation). NIST (USA) piloted the comparison. The aim of this comparison was to check the agreement of measurement of the spectral diffuse reflectance among participants, using the measurement geometry of d/0 or 0/d in the wavelength range of 360 nm to 820 nm at 20 nm increment. The comparison was a star type comparison with the samples provided by the pilot laboratory and with the measurement sequence: Pilot-Participant-Pilot. Spectralon and matte white ceramic tiles were used as the transfer standards. Each participant received three of each type of sample and at least one sample of each type was measured three times on three separate days, and the other two samples were measured once. The report presents the description of the measurement facilities, procedures and uncertainties of all the participants as well as the results of the comparison. Measurement results from the participants and their associated uncertainties were analyzed in accordance with the Guidelines for CCPR Key Comparison Report Preparation, using weighted mean with cut-off. For the calculation of the Key Comparison Reference Value (KCRV), as agreed by the participants, the data of both samples were used for the 460 nm to 820 nm region and only the data of the Spectralon samples were used in the spectral region of 360 nm to 440 nm. The unilateral degrees of equivalence (DoE) calculated for each participant are mostly consistent within the uncertainty (k = 2) of the DoE. This international comparison of spectral diffuse reflectance shows overall good agreement among all participants. Main text. To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCPR, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

Discovery of the linear region of Near Infrared Diffuse Reflectance spectra using the Kubelka-Munk theory

NASA Astrophysics Data System (ADS)

Dai, Shengyun; Pan, Xiaoning; Ma, Lijuan; Huang, Xingguo; Du, Chenzhao; Qiao, Yanjiang; Wu, Zhisheng

2018-05-01

Particle size is of great importance for the quantitative model of the NIR diffuse reflectance. In this paper, the effect of sample particle size on the measurement of harpagoside in Radix Scrophulariae powder by near infrared diffuse (NIR) reflectance spectroscopy was explored. High-performance liquid chromatography (HPLC) was employed as a reference method to construct the quantitative particle size model. Several spectral preprocessing methods were compared, and particle size models obtained by different preprocessing methods for establishing the partial least-squares (PLS) models of harpagoside. Data showed that the particle size distribution of 125-150 μm for Radix Scrophulariae exhibited the best prediction ability with R2pre=0.9513, RMSEP=0.1029 mg·g-1, and RPD = 4.78. For the hybrid granularity calibration model, the particle size distribution of 90-180 μm exhibited the best prediction ability with R2pre=0.8919, RMSEP=0.1632 mg·g-1, and RPD = 3.09. Furthermore, the Kubelka-Munk theory was used to relate the absorption coefficient k (concentration-dependent) and scatter coefficient s (particle size-dependent). The scatter coefficient s was calculated based on the Kubelka-Munk theory to study the changes of s after being mathematically preprocessed. A linear relationship was observed between k/s and absorption A within a certain range and the value for k/s was greater than 4. According to this relationship, the model was more accurately constructed with the particle size distribution of 90-180 μm when s was kept constant or in a small linear region. This region provided a good reference for the linear modeling of diffuse reflectance spectroscopy. To establish a diffuse reflectance NIR model, further accurate assessment should be obtained in advance for a precise linear model.

Scattering and/or diffusing elements in a variety of recently completed music auditoria

NASA Astrophysics Data System (ADS)

McKay, Ronald L.

2002-11-01

Architectural elements which provide effective acoustic scattering and/or diffusion in a variety of recently completed auditoria for music performance will be presented. Color slides depicting the various elements will be shown. Each will be discussed with respect to its acoustic performance and architectural logic. Measured time-energy reflection patterns will be presented in many cases.

Modifications of pancreatic diffusion MRI by tissue characteristics: what are we weighting for?

PubMed

Nissan, Noam

2017-08-01

Diffusion-weighted imaging holds the potential to improve the diagnosis and biological characterization of pancreatic disease, and in particular pancreatic cancer, which exhibits decreased values of the apparent diffusion coefficient (ADC). Yet, variable and overlapping ADC values have been reported for the healthy and the pathological pancreas, including for cancer and other benign conditions. This controversy reflects the complexity of probing the water-diffusion process in the pancreas, which is dependent upon multiple biological factors within this organ's unique physiological environment. In recent years, extensive studies have investigated the correlation between tissue properties including cellularity, vascularity, fibrosis, secretion and microstructure and pancreatic diffusivity. Understanding how the various physiological and pathological features and the underlying functional processes affect the diffusion measurement may serve to optimize the method for improved diagnostic gain. Therefore, the aim of the present review article is to elucidate the relationship between pancreatic tissue characteristics and diffusion MRI measurement. Copyright © 2017 John Wiley & Sons, Ltd.

Preliminary study of a solar selective coating system using black cobalt oxide for high temperature solar collectors

NASA Technical Reports Server (NTRS)

Mcdonald, G.

1980-01-01

Black cobalt oxide coatings (high solar absorptance layer) were deposited on thin layers of silver or gold (low emittance layer) which had been previously deposited on oxidized (diffusion barrier layer) stainless steel substrates. The reflectance properties of these coatings were measured at various thicknesses of cobalt for integrated values of the solar and infrared spectrum. The values of absorptance and emittance were calculated from the measured reflectance values, before and after exposure in air at 650 C for approximately 1000 hours. Absorptance and emittance were interdependent functions of the weight of cobalt oxide. Also, these cobalt oxide/noble metal/oxide diffusion barrier coatings have absorptances greater than 0.90 and emittances of approximately 0.20 even after about 1000 hours at 650 C.

Online determination of biophysical parameters of mucous membranes of a human body

NASA Astrophysics Data System (ADS)

Lisenko, S. A.; Kugeiko, M. M.

2013-07-01

We have developed a method for online determination of biophysical parameters of mucous membranes (MMs) of a human body (transport scattering coefficient, scattering anisotropy factor, haemoglobin concentration, degrees of blood oxygenation, average diameter of capillaries with blood) from measurements of spectral and spatial characteristics of diffuse reflection. The method is based on regression relationships between linearly independent components of the measured light signals and the unknown parameters of MMs, obtained by simulation of the radiation transfer in the MM under conditions of its general variability. We have proposed and justified the calibration-free fibre-optic method for determining the concentration of haemoglobin in MMs by measuring the light signals diffusely reflected by the tissue in four spectral regions at two different distances from the illumination spot. We have selected the optimal wavelengths of optical probing for the implementation of the method.

Optical fiber spectroscopy measures perfusion of the brain in a murine Alzheimer's disease model

NASA Astrophysics Data System (ADS)

Ahn, Hyung Jin; Strickland, Sidney; Krueger, James; Gareau, Daniel

2014-02-01

Optical fiber spectroscopy is a versatile tool for measuring diffuse reflectance and extracting absorption information that can noninvasively quantify the presence of chromophores such as oxyhemoglobin and deoxy-hemoglobin in tissues. Cerebrovascular abnormalities were widely recognized in Alzheimer's disease (AD) patients. We analyzed blood volume fraction and level of oxygenated hemoglobin in Tg6799 mice, which are transgenic mice expressing five different familial Alzheimer disease-associated mutations in the human amyloid precursor protein and presenilin-1 genes. Diffuse reflectance spectra were iteratively fit as weighted sums of oxy- and deoxy-hemoglobin. Our observations showed slightly hypoxic conditions and significantly increased blood volume in the Alzheimer's mice versus wild type. These results suggest that hyperperfusion of our AD mice may be a compensating mechanism for impaired cerebral vascular function and somehow relevant with early stage of AD patients. Ongoing work focuses on developing a cannula fixture that allows measurement in awake, behaving animals.

Speckle interferometry using fiber optic phase stepping

NASA Technical Reports Server (NTRS)

Mercer, Carolyn R.; Beheim, Glenn

1989-01-01

A system employing closed-loop phase-stepping is used to measure the out-of-plane deformation of a diffusely reflecting object. Optical fibers are used to provide reference and object beam illumination for a standard two-beam speckle interferometer, providing set-up flexibility and ease of alignment. Piezoelectric fiber-stretchers and a phase-measurement/servo system are used to provide highly accurate phase steps. Intensity data is captured with a charge-injection-device camera, and is converted into a phase map using a desktop computer. The closed-loop phase-stepping system provides 90 deg phase steps which are accurate to 0.02 deg, greatly improving this system relative to open-loop interferometers. The system is demonstrated on a speckle interferometer, measuring the rigid-body translation of a diffusely reflecting object with an accuracy + or - 10 deg, or roughly + or - 15 nanometers. This accuracy is achieved without the use of a pneumatically mounted optics table.

A one-shot-projection method for measurement of specular surfaces.

PubMed

Wang, Zhenzhou

2015-02-09

In this paper, a method is proposed to measure the shapes of specular surfaces with one-shot-projection of structured laser patterns. By intercepting the reflection of the reflected laser pattern twice with two diffusive planes, the closed form solution is achieved for each reflected ray. The points on the specular surface are reconstructed by computing the intersections of the incident rays and the reflected rays. The proposed method can measure both static and dynamic specular shapes due to its one-shot-projection, which is beyond the capability of most of state of art methods that need multiple projections. To our knowledge, the proposed method is the only method so far that could yield the closed form solutions for the dynamic and specular surfaces.

[Research on NIR equivalent spectral measurement].

PubMed

Wang, Zhi-Hong; Liu, Jie; Sun, Yu-Yang; Teng, Fei; Lin, Jun

2013-04-01

When the spectra of the diffuse reflectance of low reflectivity samples or the transmittance of low transmisivity samples are measured by a portable near infrared (NIR) spectrometer, because there is the noise of the spectrometer, the smaller the reflectance or transmittance of the sample, the lower its SNR. Even if treated by denoise methods, the spectra can not meet the requirement of NIR analysis. So the equivalent spectrum measure method was researched. Based on the intensity of the reflected or transmitted signal by the sample under the traditional measure conditions, the light current of the spectrometer was enlarged, and then the signal of the measured sample increased; the reflected or transmitted light of the measure reference was reduced to avoid the signal of the measure reference over range. Moreover the equivalent spectrum of the sample was calculated in order to make it identical with the spectrum measured by traditional method. Thus the NIR spectral SNR was improved. The results of theory analysis and experiments show that if the light signal of the spectrometer was properly increased according to the reflected or transmitted signal of the low reflectivity or transmisivity sample, the equivalent spectrum was the same as the spectrum measured by traditional method and its SNR was improved.

Translucent Radiosity: Efficiently Combining Diffuse Inter-Reflection and Subsurface Scattering.

PubMed

Sheng, Yu; Shi, Yulong; Wang, Lili; Narasimhan, Srinivasa G

2014-07-01

It is hard to efficiently model the light transport in scenes with translucent objects for interactive applications. The inter-reflection between objects and their environments and the subsurface scattering through the materials intertwine to produce visual effects like color bleeding, light glows, and soft shading. Monte-Carlo based approaches have demonstrated impressive results but are computationally expensive, and faster approaches model either only inter-reflection or only subsurface scattering. In this paper, we present a simple analytic model that combines diffuse inter-reflection and isotropic subsurface scattering. Our approach extends the classical work in radiosity by including a subsurface scattering matrix that operates in conjunction with the traditional form factor matrix. This subsurface scattering matrix can be constructed using analytic, measurement-based or simulation-based models and can capture both homogeneous and heterogeneous translucencies. Using a fast iterative solution to radiosity, we demonstrate scene relighting and dynamically varying object translucencies at near interactive rates.

Bi-color near infrared thermoreflectometry: a method for true temperature field measurement.

PubMed

Sentenac, Thierry; Gilblas, Rémi; Hernandez, Daniel; Le Maoult, Yannick

2012-12-01

In a context of radiative temperature field measurement, this paper deals with an innovative method, called bicolor near infrared thermoreflectometry, for the measurement of true temperature fields without prior knowledge of the emissivity field of an opaque material. This method is achieved by a simultaneous measurement, in the near infrared spectral band, of the radiance temperature fields and of the emissivity fields measured indirectly by reflectometry. The theoretical framework of the method is introduced and the principle of the measurements at two wavelengths is detailed. The crucial features of the indirect measurement of emissivity are the measurement of bidirectional reflectivities in a single direction and the introduction of an unknown variable, called the "diffusion factor." Radiance temperature and bidirectional reflectivities are then merged into a bichromatic system based on Kirchhoff's laws. The assumption of the system, based on the invariance of the diffusion factor for two near wavelengths, and the value of the chosen wavelengths, are then discussed in relation to a database of several material properties. A thermoreflectometer prototype was developed, dimensioned, and evaluated. Experiments were carried out to outline its trueness in challenging cases. First, experiments were performed on a metallic sample with a high emissivity value. The bidirectional reflectivity was then measured from low signals. The results on erbium oxide demonstrate the power of the method with materials with high emissivity variations in near infrared spectral band.

Image quality degradation by light-scattering processes in high-performance display devices for medical imaging

NASA Astrophysics Data System (ADS)

Badano, Aldo

1999-11-01

This thesis addresses the characterization of light scattering processes that degrade image quality in high performance electronic display devices for digital radiography. Using novel experimental and computational tools, we study the lateral diffusion of light in emissive display devices that causes extensive veiling glare and significant reduction of the physical contrast. In addition, we examine the deleterious effects of ambient light reflections that affect the contrast of low luminance regions, and superimpose unwanted structured signal. The analysis begins by introducing the performance limitations of the human visual system to define high fidelity requirements. It is noted that current devices severely suffer from image quality degradation due to optical transport processes. To model the veiling glare and reflectance characteristics of display devices, we introduce a Monte Carlo light transport simulation code, DETECT-II, that tracks individual photons through multiple scattering events. The simulation accounts for the photon polarization state at each scattering event, and provides descriptions for rough surfaces and thin film coatings. A new experimental method to measure veiling glare is described next, based on a conic collimated probe that minimizes contamination from bright areas. The measured veiling glare ratio is taken to be the luminance in the surrounding bright field divided by the luminance in the dark circle. We show that veiling glare ratios in the order of a few hundreds can be measured with an uncertainty of a few percent. The veiling glare response function is obtained by measuring the small spot contrast ratio of test patterns having varying dark spot radius. Using DETECT-II, we then estimate the ring response functions for a high performance medical imaging monitor of current design, and compare the predictions of the model with the experimentally measured response function. The data presented in this thesis demonstrate that although absorption in the faceplate of high performance monochrome cathode-ray tube monitors have reduced glare, a black matrix design is needed for high fidelity applications. For a high performance medical imaging monitor with anti-reflective coating, the glare ratio for a 1 cm diameter dark spot was measured to be 240. Finally, we introduce experimental techniques for measurements of specular and diffuse display reflectance, and we compare measured reflection coefficients with Monte Carlo estimates. A specular reflection coefficient of 0.0012, and a diffuse coefficient of 0.005 nits/lux are required to minimize degradation from ambient light in rooms with 100 lux illumination. In spite of having comparable reflection coefficients, the low maximum luminance of current devices worsens the effect of ambient light reflections when compared to radiographic film. Flat panel technologies with optimized designs can perform even better than film due to a thin faceplate, increased light absorption, and high brightness.

Report of the key-comparison of spectral diffuse reflectance (EURAMET.PR-K5) (Ref. 619)

NASA Astrophysics Data System (ADS)

Andor, György; Gál, Péter

2018-01-01

This report details the final results of the EURAMET comparison on regular spectral transmittance carried out between 2006 and 2016. The aim of this comparison was to check the agreement of measurement of the spectral diffuse reflectance among participants, using the measurement geometry of d/0 or 0/d in the wavelength range of 360 nm to 780 nm at 20 nm increment. We used a star type comparison: first the participants sent their samples to the pilot, than the pilot measured all the samples of the participants and sent them back. The participants measured the samples and sent them to the pilot for control measurement. Six standards were used as reference standards in order to maintain the scale during the comparison. These were three samples of BCR-406 opal glasses (BCR 30506; BCR 30303; BCR 30704), an MC20 Russian opal glass (MC 4777) and two samples made of pressed halon (polytetrafluoroethylene) powder (halon 2007A; halon 2007C). These six samples were designated as the Comparison reference standards. The diffuse reflectance was initially measured on the OMH (BFKH) absolute reflectometer. The link to the CCPR-K5 results was BFKH, and the check on BFKH was the PTB results who also participated in the CCPR-K5 comparison. The participants were GUM, INM, LNE, METAS, BFKH, PTB, SP. Main text To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database kcdb.bipm.org/. The final report has been peer-reviewed and approved for publication by the CCPR, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

A review of recent measurements of optical and thermal properties of α-mercuric iodide

NASA Astrophysics Data System (ADS)

Burger, A.; Morgan, S. H.; Silberman, E.; Nason, D.; Cheng, A. Y.

1992-11-01

The band gap energy of α-mercuric iodide was measured recently at elevated temperatures using optical absorption and reflection methods. In addition, reflection spectral measurements indicate that the temperature dependence of the exciton peak can provide a means of measuring, in a nondisturbing and remote manner, the local surface temperature of an α-mercuric iodide crystal during its growth from the vapor. Recent measurements of the thermal diffusivity and thermal expansion tensors have confirmed the anisotropy of this material and have implications for growth morphology and the generation of lattice defects.

Impact of morphology on diffusive dynamics on curved surfaces

NASA Astrophysics Data System (ADS)

Kusters, Remy; Storm, Cornelis

2014-03-01

Diffusive processes on nonplanar substrates are deeply relevant for cellular function and transport and increasingly used to probe and characterize the behavior of proteins in membranes. We present analytical and numerical analyses of in-plane diffusion of discrete particles on curved geometries reflecting various generic motifs in biology and explore, in particular, the effect that the shape of the substrate has on the characteristic time scales of diffusive processes. To this end, we consider both collective measures (the relaxation of concentration profiles towards equilibrium) and single-particle measures (escape rates and first passage times of individual diffusing molecules): the first relevant for the correct interpretation of FRAP experiments in curved environments; the second, for single-particle tracking probes. Each of these measures is sensitively affected by the morphology of the substrate, and we find that the exit rate out of a domain is not uniquely set by the size of its boundary, illustrating the general principle we reveal: By varying the shape of a substrate, Nature can control the diffusive time scales in a microenvironment without changing the bare substrate properties.

Improved backward ray tracing with stochastic sampling

NASA Astrophysics Data System (ADS)

Ryu, Seung Taek; Yoon, Kyung-Hyun

1999-03-01

This paper presents a new technique that enhances the diffuse interreflection with the concepts of backward ray tracing. In this research, we have modeled the diffuse rays with the following conditions. First, as the reflection from the diffuse surfaces occurs in all directions, it is impossible to trace all of the reflected rays. We confined the diffuse rays by sampling the spherical angle out of the reflected rays around the normal vector. Second, the traveled distance of reflected energy from the diffuse surface differs according to the object's property, and has a comparatively short reflection distance. Considering the fact that the rays created on the diffuse surfaces affect relatively small area, it is very inefficient to trace all of the sampled diffused rays. Therefore, we set a fixed distance as the critical distance and all the rays beyond this distance are ignored. The result of this research is that as the improved backward ray tracing can model the illumination effects such as the color bleeding effects, we can replace the radiosity algorithm under the limited environment.

Use of a multiseparation fiber optic probe for the optical diagnosis of breast cancer.

PubMed

Zhu, Changfang; Palmer, Gregory M; Breslin, Tara M; Xu, Fushen; Ramanujam, Nirmala

2005-01-01

We explore the effects of the illumination and collection geometry on optical spectroscopic diagnosis of breast cancer. Fluorescence and diffuse reflectance spectroscopy in the UV-visible spectral range are made with a multiseparation probe at three illumination-collection separations of 735, 980, and 1225 microm, respectively, from 13 malignant and 34 nonmalignant breast tissues. Statistical analysis is carried out on two types of data inputs: (1) the fluorescence and diffuse reflectance spectra recorded at each of the three illumination-collection separations and (2) the integrated fluorescence (at each excitation wavelength) or diffuse reflectance over the entire spectrum at all three illumination-collection separations. The results show that using the integrated fluorescence intensities recorded at a single excitation wavelength at all three illumination-collection separations can discriminate malignant from nonmalignant breast tissues with similar classification accuracy to that using spectral data measured at several excitation wavelengths with a single illumination-collection separation. These findings have significant implications with respect to the design of an optical system for breast cancer diagnosis. Examining the intensity attenuation at a single wavelength rather than spectral intensities at multiple wavelengths can significantly reduce the measurement and data processing time in a clinical setting as well as the cost and complexity of the optical system. Copyright 2005 Society of Photo-Optical Instrumentation Engineers.

Transmitting and reflecting diffuser. [for ultraviolet light

NASA Technical Reports Server (NTRS)

Keafer, L. S., Jr.; Burcher, E. E.; Kopia, L. P. (Inventor)

1973-01-01

A near-Lambertian diffuser is described which transmits and reflects ultraviolet light. An ultraviolet grade fused silica substrate is coated with vaporized fuse silica. The coating thickness is controlled, one thickness causing ultraviolet light to diffuse and another thickness causing ultraviolet light to reflect a near Lambertian pattern.

Dynamical measurement of refractive index distribution using digital holographic interferometry based on total internal reflection.

PubMed

Zhang, Jiwei; Di, Jianglei; Li, Ying; Xi, Teli; Zhao, Jianlin

2015-10-19

We present a method for dynamically measuring the refractive index distribution in a large range based on the combination of digital holographic interferometry and total internal reflection. A series of holograms, carrying the index information of mixed liquids adhered on a total reflection prism surface, are recorded with CCD during the diffusion process. Phase shift differences of the reflected light are reconstructed exploiting the principle of double-exposure holographic interferometry. According to the relationship between the reflection phase shift difference and the liquid index, two dimensional index distributions can be directly figured out, assuming that the index of air near the prism surface is constant. The proposed method can also be applied to measure the index of solid media and monitor the index variation during some chemical reaction processes.

Diffuse Reflectance FT-IR Of Surface Modified Kevlar

NASA Astrophysics Data System (ADS)

Benrashid, R.; Tesoro, G.; McKenzie, M. T., Jr.

1989-12-01

Diffuse reflectance FT-IR (DRIFT) has been applied to the characterization of surface modified Kevlar 29 and 49 fibers. The surface modifications include amination and sulfonation. The standard DRIFT experiment has been modified in the manner first described by Koenig et.al. 1 who used a KBR overlayer to enhance surface functional IR bands. The results from the DRIFT experiment have been correlated with those from a standard dye test. The results for degree of modification are in reasonable agreement between the two measurement approaches. However, the dye experiment is time-consuming and inconvenient. DRIFT has been shown to be useful in characterizing modified Kevlar surfaces in as-used conditions.

A method for monitoring of oxygen saturation changes in brain tissue using diffuse reflectance spectroscopy.

PubMed

Rejmstad, Peter; Johansson, Johannes D; Haj-Hosseini, Neda; Wårdell, Karin

2017-03-01

Continuous measurement of local brain oxygen saturation (SO 2 ) can be used to monitor the status of brain trauma patients in the neurocritical care unit. Currently, micro-oxygen-electrodes are considered as the "gold standard" in measuring cerebral oxygen pressure (pO 2 ), which is closely related to SO 2 through the oxygen dissociation curve (ODC) of hemoglobin, but with the drawback of slow in response time. The present study suggests estimation of SO 2 in brain tissue using diffuse reflectance spectroscopy (DRS) for finding an analytical relation between measured spectra and the SO 2 for different blood concentrations. The P 3 diffusion approximation is used to generate a set of spectra simulating brain tissue for various levels of blood concentrations in order to estimate SO 2 . The algorithm is evaluated on optical phantoms mimicking white brain matter (blood volume of 0.5-2%) where pO 2 and temperature is controlled and on clinical data collected during brain surgery. The suggested method is capable of estimating the blood fraction and oxygen saturation changes from the spectroscopic signal and the hemoglobin absorption profile. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Feynman-Kac equations for reaction and diffusion processes

NASA Astrophysics Data System (ADS)

Hou, Ru; Deng, Weihua

2018-04-01

This paper provides a theoretical framework for deriving the forward and backward Feynman-Kac equations for the distribution of functionals of the path of a particle undergoing both diffusion and reaction processes. Once given the diffusion type and reaction rate, a specific forward or backward Feynman-Kac equation can be obtained. The results in this paper include those for normal/anomalous diffusions and reactions with linear/nonlinear rates. Using the derived equations, we apply our findings to compute some physical (experimentally measurable) statistics, including the occupation time in half-space, the first passage time, and the occupation time in half-interval with an absorbing or reflecting boundary, for the physical system with anomalous diffusion and spontaneous evanescence.

Diffusion tensor imaging demonstrates brainstem and cerebellar abnormalities in congenital central hypoventilation syndrome.

PubMed

Kumar, Rajesh; Macey, Paul M; Woo, Mary A; Alger, Jeffry R; Harper, Ronald M

2008-09-01

Congenital central hypoventilation syndrome (CCHS) patients show reduced breathing drive during sleep, decreased hypoxic and hypercapnic ventilatory responses, and autonomic and affective deficits, suggesting both brainstem and forebrain injuries. Forebrain damage was previously described in CCHS, but methodological limitations precluded detection of brainstem injury, a concern because genetic mutations in CCHS target brainstem autonomic nuclei. To assess brainstem and cerebellar areas, we used diffusion tensor imaging-based measures, namely axial diffusivity, reflecting water diffusion parallel to fibers, and sensitive to axonal injury, and radial diffusivity, measuring diffusion perpendicular to fibers, and indicative of myelin injury. Diffusion tensor imaging was performed in 12 CCHS and 26 controls, and axial and radial diffusivity maps were compared between groups using analysis of covariance (covariates; age and gender). Increased axial diffusivity in CCHS appeared within the lateral medulla and clusters with injury extended from the dorsal midbrain through the periaqueductal gray, raphé, and superior cerebellar decussation, ventrally to the basal-pons. Cerebellar cortex and deep nuclei, and the superior and inferior cerebellar peduncles showed increased radial diffusivity. Midbrain, pontine, and lateral medullary structures, and the cerebellum and its fiber systems are injured in CCHS, likely contributing to the characteristics found in the syndrome.

ESTABLISHING BRDF CALIBRATION CAPABILITIES THROUGH SHORTWAVE INFRARED

PubMed Central

Georgiev, Georgi T.; Butler, James J.; Thome, Kurt; Cooksey, Catherine; Ding, Leibo

2017-01-01

Satellite instruments operating in the reflective solar wavelength region require accurate and precise determination of the Bidirectional Reflectance Distribution Functions (BRDFs) of the laboratory and flight diffusers used in their pre-flight and on-orbit calibrations. This paper advances that initial work and presents a comparison of spectral Bidirectional Reflectance Distribution Function (BRDF) and Directional Hemispherical Reflectance (DHR) of Spectralon*, a common material for laboratory and on-orbit flight diffusers. A new measurement setup for BRDF measurements from 900 nm to 2500 nm located at NASA Goddard Space Flight Center (GSFC) is described. The GSFC setup employs an extended indium gallium arsenide detector, bandpass filters, and a supercontinuum light source. Comparisons of the GSFC BRDF measurements in the shortwave infrared (SWIR) with those made by the National Institute of Standards and Technology (NIST) Spectral Tri-function Automated Reference Reflectometer (STARR) are presented. The Spectralon sample used in this study was 2 inch diameter, 99% white pressed and sintered Polytetrafluoroethylene (PTFE) target. The NASA/NIST BRDF comparison measurements were made at an incident angle of 0° and viewing angle of 45°. Additional BRDF data not compared to NIST were measured at additional incident and viewing angle geometries and are not presented here. The total combined uncertainty for the measurement of BRDF in the SWIR range made by the GSFC scatterometer is less than 1% (k = 1). This study is in support of the calibration of the Radiation Budget Instrument (RBI) and Visible Infrared Imaging Radiometer Suit (VIIRS) instruments of the Joint Polar Satellite System (JPSS) and other current and future NASA remote sensing missions operating across the reflected solar wavelength region. PMID:29167593

Silver photo-diffusion and photo-induced macroscopic surface deformation of Ge{sub 33}S{sub 67}/Ag/Si substrate

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sakaguchi, Y., E-mail: y-sakaguchi@cross.or.jp; Asaoka, H.; Uozumi, Y.

2016-08-07

Ge-chalcogenide films show various photo-induced changes, and silver photo-diffusion is one of them which attracts lots of interest. In this paper, we report how silver and Ge-chalcogenide layers in Ge{sub 33}S{sub 67}/Ag/Si substrate stacks change under light exposure in the depth by measuring time-resolved neutron reflectivity. It was found from the measurement that Ag ions diffuse all over the matrix Ge{sub 33}S{sub 67} layer once Ag dissolves into the layer. We also found that the surface was macroscopically deformed by the extended light exposure. Its structural origin was investigated by a scanning electron microscopy.

Effect of diffusive and nondiffusive surfaces combinations on sound diffusion

NASA Astrophysics Data System (ADS)

Shafieian, Masoume; Kashani, Farokh Hodjat

2010-05-01

One of room acoustic goals, especially in small to medium rooms, is sound diffusion in low frequencies, which have been the subject of lots of researches. Sound diffusion is a very important consideration in acoustics because it minimizes the coherent reflections that cause problems. It also tends to make an enclosed space sound larger than it is. Diffusion is an excellent alternative or complement to sound absorption in acoustic treatment because it doesn’t really remove much energy, which means it can be used to effectively reduce reflections while still leaving an ambient or live sounding space. Distribution of diffusive and nondiffusive surfaces on room walls affect sound diffusion in room, but the amount, combination, and location of these surfaces are still the matter of question. This paper investigates effects of these issues on room acoustic frequency response in different parts of the room with different source-receiver locations. Room acoustic model based on wave method is used (implemented) which is very accurate and convenient for low frequencies in such rooms. Different distributions of acoustic surfaces on room walls have been introduced to the model and room frequency response results are calculated. For the purpose of comparison, some measurements results are presented. Finally for more smooth frequency response in small and medium rooms, some suggestions are made.

Stone/tissue differentiation during intracorporeal lithotripsy using diffuse white light reflectance spectroscopy: In vitro and clinical measurements.

PubMed

Lange, Birgit; Jocham, Dieter; Brinkmann, Ralf; Cordes, Jens

2014-10-01

Holmium laser lithotripsy is the 'gold standard' for intracorporeal fragmentation of stones. However, there is a risk of damaging and perforating the ureter wall when the laser is accidentally fired while the fiber is in contact with tissue. The aim of this study was to evaluate if white illumination light, diffusely reflected back into the treatment fiber and spectrally analyzed, can be used for differentiating between stone and tissue. Firstly, in vitro reflectance spectra (Xenon light source, wavelength range λ = 350-850 nm) of 38 human kidney stones, porcine renal calix and ureter tissue were collected. Secondly, in an in vivo study with 8 patients, 72 ureter and 49 stone reflectance signals were recorded during endourological interventions. The spectra were analyzed to discriminate between stone and tissue by the absence or presence of minima due to hemoglobin absorption at λ1  = 542nm and λ3  = 576nm. In vitro, all stone and tissue signals could correctly be identified by calculating the ratio R = I (λ1  = 542 nm)/I (λ2  = 475 nm): Because of the hemoglobin absorption at λ1 , R is smaller for tissue than for calculi. In vivo, only 75% tissue spots could correctly be identified utilizing this method. Using the more sophisticated evaluation of looking for minima in the diffuse reflectance spectra at λ1  = 542 nm and λ3  = 576 nm, 62 out of 64 tissue spots were correctly identified (sensitivity 96.9%). This was also the case for 39 out of 43 stone spots. Taking into account the number of measured spectra, a tissue detection probability of 91% and a stone detection probability of 77% was achieved (significance level 5%). White light diffusely reflected off the treatment zone into the fiber can be used to strongly improve the safety of Holmium laser lithotripsy by implementing an automatic feedback control algorithm that averts mispositioning the fiber. © 2014 Wiley Periodicals, Inc.

The Value Of Different Standards And Measurement Systems To Determine The Optical Properties Of Paper And For Standardized Color Reproduction

NASA Astrophysics Data System (ADS)

Sobotica, Werner

1990-06-01

Several terms are used to determine the optical properties of paper and board. Brightness, whiteness, diffuse reflectance factor, diffuse blue reflectance factor, specular gloss, spectral reflectivity and whiteness and yellowness are the used terms. The mainly used term is brightness of paper, an important property in many specifications, but this property has no visual perceptual foundation. Instead it is based on the filter chosen to measure the reflectance of pulp in the region most sensitive to the effects, of bleaching. Therefore an increasing demand for a specification of whiteness can be observed. Belonging to the concept of color, whiteness has to be measured and interpreted in the scope of CIE (Commission Internationale l'Eclairage) based on colorimetri tristimulus data transformed to agree with the perceptual assessment of whiteness. Human observers differentiate between the whiteness of the object being viewed and its brightness. A special index of whiteness is needed. No general agreement was so far reached regarding the best index which could and should be adopted universally among the two recommended by the CIE. Nearly every white sample is a little colored, having bluish, greenish or redish tint. In the post no numerical measure of tint was assigned to any of the whiteness indexes. Further problems arise that fluorescent whiteness agents are added to paper and board to increase their whiteness. This requires not only instrumental design and calibration but also the illuminant to be adopted for such measurements.

Noninvasive detection of skin cancers by measuring optical properties of tissues

NASA Astrophysics Data System (ADS)

Wang, Lihong V.; Jacques, Steven L.

1995-05-01

Skin cancer is the most frequently occurring cancer of all cancers. Each yea rover 500,000 new cases of skin cancer will be detected. A high percentage of skin cancers are diseases in which fatalities can be all but eliminated and morbidity reduced if detected early and treated properly. These skin lesions are distinguished generally by subjective visual inspection and their definitive diagnosis requires time-consuming expensive histopathological evaluation of excisional or incisional biopsies. In vivo experimental evidence published in the literature has shown that cancerous skin lesions have different total diffuse reflectance spectra than non- cancerous lesions or normal skin. Therefore, cancerous skin lesions may be differentiated from non-cancerous skin lesions by comparing the optical properties of the skin lesions with those of the surrounding normal skin sites, where the optical properties of the normal skin sites are used to account for different types of skin or different areas of skin. We have demonstrated that the effect of melanin concentration on the diffuse reflectance may be removed by extrapolating the reflectance at different wavelengths to an apparent pivot point. Because the concentration of melanin does not indicate malignancy, the removal of its effect is important to avoid false detection. The total diffuse reflectance depends on the albedo and anisotropy of tissues. Therefore, the total diffuse reflectance will remain the same as long as the anisotropy and the ratio between the absorption coefficient and the reduced scattering coefficient remain the same. Separating the absorption and scattering effects should enhance the detection sensitivity of skin cancers.

Gold nanorods based diffusion reflection measurements: current status and perspectives for clinical applications

NASA Astrophysics Data System (ADS)

Ankri, Rinat; Fixler, Dror

2017-07-01

Optical imaging is a powerful tool for investigating the structure and function of tissues. Tissue optical imaging technologies are generally discussed under two broad regimes: microscopic and macroscopic, while the latter is widely investigated in the field of light-tissue interaction. Among the developed optical technologies for tissue investigation, the diffusion reflectance (DR) method is a simple and safe technology. However, this method suffers from low specificity and low signal-to-noise ratio, so the extraction of the tissue properties is not an easy task. In this review, we describe the use of gold nanorods (GNRs) in DR spectroscopy. The GNRs present unique optical properties which enhance the scattering and absorption properties of a tissue. The GNRs can be easily targeted toward abnormal sites in order to improve the DR signal and to distinguish between the healthy and the abnormal sites in the tissue, with high specificity. This article describes the use of the DR-GNRs method for the detection of cancer and atherosclerosis, from light transfer theory, through the extraction of the tissue properties using the diffusion theory and up to DR in vivo measurements.

A Traceable Ground to On-Orbit Radiometric Calibration System for the Solar Reflective Wavelength Region

NASA Technical Reports Server (NTRS)

Heath, Donald F.; Georgiev, Georgi

2012-01-01

This paper describes the combination of a Mie scattering spectral BSDF and BTDF albedo standard whose calibration is traceable to the NIST SIRCUS Facility or the NIST STARR II Facility. The Space-based Calibration Transfer Spectroradiometer (SCATS) sensor uses a simple, invariant optical configuration and dedicated narrow band spectral channel modules to provide very accurate, polarization-insensitive, stable measurements of earth albedo and lunar disk albedo. Optical degradation effects on calibration stability are eliminated through use of a common optical system for observations of the Sun, Earth, and Moon. The measurements from space would be traceable to SI units through preflight calibrations of radiance and irradiance at NIST's SIRCUS facility and the invariant optical system used in the sensor. Simultaneous measurements are made in multiple spectral channels covering the solar reflective wavelength range of 300 nm to 2.4 microns. The large dynamic range of signals is handled by use of single-element, highly-linear detectors, stable discrete electronic components, and a non imaging optical configuration. Up to 19 spectral modules can be mounted on a single-axis drive to give direct pointing at the Earth and at least once per orbit view of the Sun and Moon. By observing the Sun on every orbit, the most stringent stability requirements of the system are limited to short time periods. The invariant optical system for both radiance and irradiance measurements also give excellent transfer to-orbit SI traceability. Emerging instrumental requirements for remotely sensing tropospheric trace species have led to a rethinking by some of the paradigm for Systeme International d'Unites (SI) traceability of the spectral irradiance and radiance radiometric calibrations to spectral albedo (sr(exp -1)) which is not a SI unit. In the solar reflective wavelength region the spectral albedo calibrations are tied often to either the spectral albedo of a solar diffuser or the Moon. This new type of Mie scattering diffuser (MSD) is capable of withstanding high temperatures, and is more Lambertian than Spectralon(tm). It has the potential of covering the entire solar reflective wavelength region. Laboratory measurements have shown that the specular reflectance component is negligible, and indicate that internal absorption by multiple scattering is small. This MSD, a true volume diffuser, exhibits a high degree of radiometric stability which suggests that measurements at the National Institute of Standards and Technology (NIST) could provide a spectral albedo standard. Measurements have been made of its radiometric stability under a simulated space environment of high energy gamma rays, high energy protons, and UV radiation from ambient down to the vacuum ultraviolet H Lyman alpha at 121.6 nm for its eventual use in space as a solar diffuser.

Verification of reflectance models in turbid waters

NASA Technical Reports Server (NTRS)

Tanis, F. J.; Lyzenga, D. R.

1981-01-01

Inherent optical parameters of very turbid waters were used to evaluate existing water reflectance models. Measured upwelling radiance spectra and Monte Carlo simulations of the radiative transfer equations were compared with results from models based upon two flow, quasi-single scattering, augmented isotropic scattering, and power series approximation. Each model was evaluated for three separate components of upwelling radiance: (1) direct sunlight; (2) diffuse skylight; and (3) internally reflected light. Limitations of existing water reflectance models as applied to turbid waters and possible applications to the extraction of water constituent information are discussed.

Deposition and characterization of B4C/CeO2 multilayers at 6.x nm extreme ultraviolet wavelengths

NASA Astrophysics Data System (ADS)

Sertsu, M. G.; Giglia, A.; Brose, S.; Park, D.; Wang, Z. S.; Mayer, J.; Juschkin, L.; Nicolosi, P.

2016-03-01

New multilayers of boron carbide/cerium dioxide (B4C/CeO2) combination on silicon (Si) substrate are manufactured to represent reflective-optics candidates for future lithography at 6.x nm wavelength. This is one of only a few attempts to make multilayers of this kind. Combination of several innovative experiments enables detailed study of optical properties, structural properties, and interface profiles of the multilayers in order to open up a room for further optimization of the manufacturing process. The interface profile is visualized by high-angle annular dark-field imaging which provides highly sensitive contrast to atomic number. Synchrotron based at-wavelength extreme ultraviolet (EUV) reflectance measurements near the boron (B) absorption edge allow derivation of optical parameters with high sensitivity to local atom interactions. X-ray reflectivity measurements at Cu-Kalpha (8 keV ) determine the period of multilayers with high in-depth resolution. By combining these measurements and choosing robust nonlinear curve fitting algorithms, accuracy of the results has been significantly improved. It also enables a comprehensive characterization of multilayers. Interface diffusion is determined to be a major cause for the low reflectivity performance. Optical constants of B4C and CeO2 layers are derived in EUV wavelengths. Besides, optical properties and asymmetric thicknesses of inter-diffusion layers (interlayers) in EUV wavelengths near the boron edge are determined. Finally, ideal reflectivity of the B4C/CeO2 combination is calculated by using optical constants derived from the proposed measurements in order to evaluate the potentiality of the design.

Behavior of optical properties of coagulated blood sample at 633 nm wavelength

NASA Astrophysics Data System (ADS)

Morales Cruzado, Beatriz; Vázquez y Montiel, Sergio; Delgado Atencio, José Alberto

2011-03-01

Determination of tissue optical parameters is fundamental for application of light in either diagnostics or therapeutical procedures. However, in samples of biological tissue in vitro, the optical properties are modified by cellular death or cellular agglomeration that can not be avoided. This phenomena change the propagation of light within the biological sample. Optical properties of human blood tissue were investigated in vitro at 633 nm using an optical setup that includes a double integrating sphere system. We measure the diffuse transmittance and diffuse reflectance of the blood sample and compare these physical properties with those obtained by Monte Carlo Multi-Layered (MCML). The extraction of the optical parameters: absorption coefficient μa, scattering coefficient μs and anisotropic factor g from the measurements were carried out using a Genetic Algorithm, in which the search procedure is based in the evolution of a population due to selection of the best individual, evaluated by a function that compares the diffuse transmittance and diffuse reflectance of those individuals with the experimental ones. The algorithm converges rapidly to the best individual, extracting the optical parameters of the sample. We compare our results with those obtained by using other retrieve procedures. We found that the scattering coefficient and the anisotropic factor change dramatically due to the formation of clusters.

Integrated three-dimensional shape and reflection properties measurement system.

PubMed

Krzesłowski, Jakub; Sitnik, Robert; Maczkowski, Grzegorz

2011-02-01

Creating accurate three-dimensional (3D) digitalized models of cultural heritage objects requires that information about surface geometry be integrated with measurements of other material properties like color and reflectance. Up until now, these measurements have been performed in laboratories using manually integrated (subjective) data analyses. We describe an out-of-laboratory bidirectional reflectance distribution function (BRDF) and 3D shape measurement system that implements shape and BRDF measurement in a single setup with BRDF uncertainty evaluation. The setup aligns spatial data with the angular reflectance distribution, yielding a better estimation of the surface's reflective properties by integrating these two modality measurements into one setup using a single detector. This approach provides a better picture of an object's intrinsic material features, which in turn produces a higher-quality digitalized model reconstruction. Furthermore, this system simplifies the data processing by combining structured light projection and photometric stereo. The results of our method of data analysis describe the diffusive and specular attributes corresponding to every measured geometric point and can be used to render intricate 3D models in an arbitrarily illuminated scene.

Reflected Sunlight Reduction and Characterization for a Deep-Space Optical Receiver Antenna (DSORA)

NASA Technical Reports Server (NTRS)

Clymer, B. D.

1990-01-01

A baffle system for the elimination of first-order specular and diffuse reflection of sunlight from the sunshade of a deep-space optical receiver telescope is presented. This baffle system consists of rings of 0.5cm blades spaced 2.5 cm apart on the walls of GO hexagonal sunshade tubes that combine to form the telescope sunshade. The shadow cast by the blades, walls, and rims of the tubes prevent all first-order reflections of direct sunlight from reaching the primary mirror of the telescope. A reflection model of the sunshade without baffles is also presented for comparison. Since manufacturers of absorbing surfaces do not measure data near grazing incidence, the reflection properties at anticipated angles of incidence must be characterized. A description of reflection from matte surfaces in term of bidirectional reflection distribution function (BRDF) is presented along with a discussion of measuring BRDF near grazing incidence.

Diffuse reflectance spectroscopy of liver tissue

NASA Astrophysics Data System (ADS)

Reistad, Nina; Nilsson, Jan; Vilhelmsson Timmermand, Oskar; Sturesson, Christian; Andersson-Engels, Stefan

2015-06-01

Diffuse reflectance spectroscopy (DRS) with a fiber-optic contact probe is a cost-effective, rapid, and non-invasive optical method used to extract diagnosis information of tissue. By combining commercially available VIS- and NIR-spectrometers with various fiber-optic contact-probes, we have access to the full wavelength range from around 400 to 1600 nm. Using this flexible and portable spectroscopy system, we have acquired ex-vivo DRS-spectra from murine, porcine, and human liver tissue. For extracting the tissue optical properties from the measured spectra, we have employed and compared predictions from two models for light propagation in tissue, diffusion theory model (DT) and Monte Carlo simulations (MC). The focus in this work is on the capacity of this DRS-technique in discriminating metastatic tumor tissue from normal liver tissue as well as in assessing and characterizing damage to non-malignant liver tissue induced by preoperative chemotherapy for colorectal liver metastases.

Quantitative skin color measurements in acanthosis nigricans patients: colorimetry and diffuse reflectance spectroscopy.

PubMed

Pattamadilok, Bensachee; Devpura, Suneetha; Syed, Zain U; Agbai, Oma N; Vemulapalli, Pranita; Henderson, Marsha; Rehse, Steven J; Mahmoud, Bassel H; Lim, Henry W; Naik, Ratna; Hamzavi, Iltefat H

2012-08-01

Tristimulus colorimetry and diffuse reflectance spectroscopy (DRS) are white-light skin reflectance techniques used to measure the intensity of skin pigmentation. The tristimulus colorimeter is an instrument that measures a perceived color and the DRS instrument measures biological chromophores of the skin, including oxy- and deoxyhemoglobin, melanin and scattering. Data gathered from these tools can be used to understand morphological changes induced in skin chromophores due to conditions of the skin or their treatments. The purpose of this study was to evaluate the use of these two instruments in color measurements of acanthosis nigricans (AN) lesions. Eight patients with hyperinsulinemia and clinically diagnosable AN were seen monthly. Skin pigmentation was measured at three sites: the inner forearm, the medial aspect of the posterior neck, and anterior neck unaffected by AN. Of the three, measured tristimulus L*a*b* color parameters, the luminosity parameter L* was found to most reliably distinguish lesion from normally pigmented skin. The DRS instrument was able to characterize a lesion on the basis of the calculated melanin concentration, though melanin is a weak indicator of skin change and not a reliable measure to be used independently. Calculated oxyhemoglobin and deoxyhemoglobin concentrations were not found to be reliable indicators of AN. Tristimulus colorimetry may provide reliable methods for respectively quantifying and characterizing the objective color change in AN, while DRS may be useful in characterizing changes in skin melanin content associated with this skin condition. © 2012 John Wiley & Sons A/S.

Toward reliable retrieval of functional information of papillary dermis using spatially resolved diffuse reflectance spectroscopy

PubMed Central

Chen, Yu-Wen; Guo, Jun-Yen; Tzeng, Shih-Yu; Chou, Ting-Chun; Lin, Ming-Jen; Huang, Lynn Ling-Huei; Yang, Chao-Chun; Hsu, Chao-Kai; Tseng, Sheng-Hao

2016-01-01

Spatially resolved diffuse reflectance spectroscopy (SRDRS) has been employed to quantify tissue optical properties and its interrogation volume is majorly controlled by the source-to-detector separations (SDSs). To noninvasively quantify properties of dermis, a SRDRS setup that includes SDS shorter than 1 mm is required. It will be demonstrated in this study that Monte Carlo simulations employing the Henyey-Greenstein phase function cannot always precisely predict experimentally measured diffuse reflectance at such short SDSs, and we speculated this could be caused by the non-negligible backward light scattering at short SDSs that cannot be properly modeled by the Henyey-Greenstein phase function. To accurately recover the optical properties and functional information of dermis using SRDRS, we proposed the use of the modified two-layer (MTL) geometry. Monte Carlo simulations and phantom experiment results revealed that the MTL probing geometry was capable of faithfully recovering the optical properties of upper dermis. The capability of the MTL geometry in probing the upper dermis properties was further verified through a swine study, and it was found that the measurement results were reasonably linked to histological findings. Finally, the MTL probe was utilized to study psoriatic lesions. Our results showed that the MTL probe was sensitive to the physiological condition of tissue volumes within the papillary dermis and could be used in studying the physiology of psoriasis. PMID:26977361

Toward reliable retrieval of functional information of papillary dermis using spatially resolved diffuse reflectance spectroscopy.

PubMed

Chen, Yu-Wen; Guo, Jun-Yen; Tzeng, Shih-Yu; Chou, Ting-Chun; Lin, Ming-Jen; Huang, Lynn Ling-Huei; Yang, Chao-Chun; Hsu, Chao-Kai; Tseng, Sheng-Hao

2016-02-01

Spatially resolved diffuse reflectance spectroscopy (SRDRS) has been employed to quantify tissue optical properties and its interrogation volume is majorly controlled by the source-to-detector separations (SDSs). To noninvasively quantify properties of dermis, a SRDRS setup that includes SDS shorter than 1 mm is required. It will be demonstrated in this study that Monte Carlo simulations employing the Henyey-Greenstein phase function cannot always precisely predict experimentally measured diffuse reflectance at such short SDSs, and we speculated this could be caused by the non-negligible backward light scattering at short SDSs that cannot be properly modeled by the Henyey-Greenstein phase function. To accurately recover the optical properties and functional information of dermis using SRDRS, we proposed the use of the modified two-layer (MTL) geometry. Monte Carlo simulations and phantom experiment results revealed that the MTL probing geometry was capable of faithfully recovering the optical properties of upper dermis. The capability of the MTL geometry in probing the upper dermis properties was further verified through a swine study, and it was found that the measurement results were reasonably linked to histological findings. Finally, the MTL probe was utilized to study psoriatic lesions. Our results showed that the MTL probe was sensitive to the physiological condition of tissue volumes within the papillary dermis and could be used in studying the physiology of psoriasis.

Autofluorescence and diffuse reflectance patterns in cervical spectroscopy

NASA Astrophysics Data System (ADS)

Marin, Nena Maribel

Fluorescence and diffuse reflectance spectroscopy are two new optical technologies, which have shown promise to aid in the real time, non-invasive identification of cancers and precancers. Spectral patterns carry a fingerprint of scattering, absorption and fluorescence properties in tissue. Scattering, absorption and fluorescence in tissue are directly affected by biological features that are diagnostically significant, such as nuclear size, micro-vessel density, volume fraction of collagen fibers, tissue oxygenation and cell metabolism. Thus, analysis of spectral patterns can unlock a wealth of information directly related with the onset and progression of disease. Data from a Phase II clinical trial to assess the technical efficacy of fluorescence and diffuse reflectance spectroscopy acquired from 850 women at three clinical locations with two research grade optical devices is calibrated and analyzed. Tools to process and standardize spectra so that data from multiple spectrometers can be combined and analyzed are presented. Methodologies for calibration and quality assurance of optical systems are established to simplify design issues and ensure validity of data for future clinical trials. Empirically based algorithms, using multivariate statistical approaches are applied to spectra and evaluated as a clinical diagnostic tool. Physically based algorithms, using mathematical models of light propagation in tissue are presented. The presented mathematical model combines a diffusion theory in P3 approximation reflectance model and a 2-layer fluorescence model using exponential attenuation and diffusion theory. The resulting adjoint fluorescence and reflectance model extracts twelve optical properties characterizing fluorescence efficiency of cervical epithelium and stroma fluorophores, stromal hemoglobin and collagen absorption, oxygen saturation, and stromal scattering strength and shape. Validations with Monte Carlo simulations show that adjoint model extracted optical properties of the epithelium and the stroma can be estimated accurately. Adjoint model is applied to 926 clinical measurements from 503 patients. Mean values of extracted optical properties have demonstrated to characterize the biological changes associated with dysplastic progression. Finally, penalized logistic regression algorithms are applied to discriminate dysplastic stages in tissue based on extracted optical features. This work provides understandable and interpretable information regarding predictive and generalization ability of optical spectroscopy in neoplastic changes using a minimum subset of optical measurements. Ultimately these methodologies would facilitate the transfer of these optical technologies into clinical practice.

Effect of surface topographic features on the optical properties of skin: a phantom study

NASA Astrophysics Data System (ADS)

Liu, Guangli; Chen, Jianfeng; Zhao, Zuhua; Zhao, Gang; Dong, Erbao; Chu, Jiaru; Xu, Ronald X.

2016-10-01

Tissue-simulating phantoms are used to validate and calibrate optical imaging systems and to understand light transport in biological tissue. Light propagation in a strongly turbid medium such as skin tissue experiences multiple scattering and diffuse reflection from the surface. Surface roughness introduces phase shifts and optical path length differences for light which is scattered within the skin tissue and reflected from the surface. In this paper, we study the effect of mismatched surface roughness on optical measurement and subsequent determination of optical properties of skin tissue. A series of phantoms with controlled surface features and optical properties corresponding to normal human skin are fabricated. The fabrication of polydimethylsiloxane (PDMS) phantoms with known surface roughness follows a standard soft lithography process. Surface roughness of skin-simulating phantoms are measured with Bruker stylus profiler. The diffuse reflectance of the phantom is validated by a UV/VIS spectrophotometer. The results show that surface texture and roughness have considerable influence on the optical characteristics of skin. This study suggests that surface roughness should be considered as an important contributing factor for the determination of tissue optical properties.

Simultaneous in vivo imaging of diffuse optical reflectance, optoacoustic pressure and ultrasonic scattering (Conference Presentation)

NASA Astrophysics Data System (ADS)

Subochev, Pavel V.; Orlova, Anna G.; Turchin, Ilya V.

2017-03-01

We will present reflection-mode bioimaging system providing complementary optical, photoacsoutic and acoustic measurements by acoustic detector after each laser pulse with 2kHz repetition rate. The photons absorbed within the biological tissue provide optoacoustic (OA) signals, the photons absorbed by the external electrode of a detector provide the measurable diffuse reflectance (DR) from the sample and the probing ultrasonic (US) pulse. To demonstrate the in vivo capabilities of the system we performed complementary DR/OA/US imaging of small laboratory animals and human palm with 3.5mm/50μm/35μm lateral resolution at up to 3 mm diagnostic depth. Functional OA and DR imaging demonstrated the levels of tissue vascularization and blood supply. Structural US imaging was essential for understanding the position of vessels and zones with different perfusion. Before BiOS-2017 we plan to accomplish more in vivo experiments validating the developed triple-modality system as diagnostic tool to detect vascularization as well as mechanisms of vascular changes when monitoring response to therapy.

Diffuse Reflectance Spectroscopy Versus Mexameter(®) MX18 Measurements of Melanin and Erythema in an African Population.

PubMed

Wright, Caradee Y; Karsten, Aletta E; Wilkes, Marcus; Singh, Ann; du Plessis, Johan; Albers, Patricia N; Karsten, Petrus A

2016-07-01

Melanin provides protection against excess exposure to solar ultraviolet radiation (UVR) and related adverse health effects. Diffuse reflectance spectroscopy (DRS) can be used to calculate cutaneous melanin and erythema, but this is complex and has been mostly used for light-to-medium pigmented skin. Handheld reflectance spectrophotometers, such as the Mexameter(®) MX18, can also be used. We compared DRS-calculated melanin and erythema values with Mexameter melanin and erythema index values to understand how these techniques/measurements correlate in an African population of predominantly deeply pigmented skin. Five hundred and three participants comprised 68.5% self-identified Black African, 9.9% Indian/Asian, 18.4% White and 2.9% Colored. The majority of Black African (45%), Indian/Asian (34%) and Colored (53%) participants self-identified their skin as being "brown." Measured melanin levels increased with darker self-reported skin color. DRS-calculated and Mexameter melanin values demonstrated a positive correlation (Spearman rho = 0.87, P < 0.001). The results from both instruments showed erythema values were strongly correlated with their own melanin values. This finding is considered spurious and may result from the complexity of separating brown and red pigment when using narrowband reflectance techniques. Further work is needed to understand melanin, erythema and color in Black skin given sun-related health risks in vulnerable groups in Africa. © 2016 The American Society of Photobiology.

Multi-spectral imaging of oxygen saturation

NASA Astrophysics Data System (ADS)

Savelieva, Tatiana A.; Stratonnikov, Aleksander A.; Loschenov, Victor B.

2008-06-01

The system of multi-spectral imaging of oxygen saturation is an instrument that can record both spectral and spatial information about a sample. In this project, the spectral imaging technique is used for monitoring of oxygen saturation of hemoglobin in human tissues. This system can be used for monitoring spatial distribution of oxygen saturation in photodynamic therapy, surgery or sports medicine. Diffuse reflectance spectroscopy in the visible range is an effective and extensively used technique for the non-invasive study and characterization of various biological tissues. In this article, a short review of modeling techniques being currently in use for diffuse reflection from semi-infinite turbid media is presented. A simple and practical model for use with a real-time imaging system is proposed. This model is based on linear approximation of the dependence of the diffuse reflectance coefficient on relation between absorbance and reduced scattering coefficient. This dependence was obtained with the Monte Carlo simulation of photon propagation in turbid media. Spectra of the oxygenated and deoxygenated forms of hemoglobin differ mostly in the red area (520 - 600 nm) and have several characteristic points there. Thus four band-pass filters were used for multi-spectral imaging. After having measured the reflectance, the data obtained are used for fitting the concentration of oxygenated and free hemoglobin, and hemoglobin oxygen saturation.

Rapid determination of ions by combined solid-phase extraction--diffuse reflectance spectroscopy

NASA Technical Reports Server (NTRS)

Fritz, James S.; Arena, Matteo P.; Steiner, Steven A.; Porter, Marc D.

2003-01-01

We introduce colorimetric solid-phase extraction (C-SPE) for the rapid determination of selected ions. This new technique links the exhaustive concentration of an analyte by SPE onto a membrane disk surface for quantitative measurement with a hand-held diffuse reflectance spectrometer. The concentration/measurement procedure is complete in approximately 1 min and can be performed almost anywhere. This method has been used to monitor iodine and iodide in spacecraft water in the 0.1-5.0 ppm range and silver(I) in the range of 5.0-1000 microg/l. Applications to the trace analysis of copper(II), nickel(II), iron(III) and chromium(VI) are described. Studies on the mechanism of extraction showed that impregnation of the disk with a surfactant as well as a complexing reagent results in uptake of additional water, which markedly improves the extraction efficiency.

Use of visible, near-infrared, and thermal infrared remote sensing to study soil moisture

NASA Technical Reports Server (NTRS)

Blanchard, M. B.; Greeley, R.; Goettelman, R.

1974-01-01

Two methods are described which are used to estimate soil moisture remotely using the 0.4- to 14.0 micron wavelength region: (1) measurement of spectral reflectance, and (2) measurement of soil temperature. The reflectance method is based on observations which show that directional reflectance decreases as soil moisture increases for a given material. The soil temperature method is based on observations which show that differences between daytime and nighttime soil temperatures decrease as moisture content increases for a given material. In some circumstances, separate reflectance or temperature measurements yield ambiguous data, in which case these two methods may be combined to obtain a valid soil moisture determination. In this combined approach, reflectance is used to estimate low moisture levels; and thermal inertia (or thermal diffusivity) is used to estimate higher levels. The reflectance method appears promising for surface estimates of soil moisture, whereas the temperature method appears promising for estimates of near-subsurface (0 to 10 cm).

Use of visible, near-infrared, and thermal infrared remote sensing to study soil moisture

NASA Technical Reports Server (NTRS)

Blanchard, M. B.; Greeley, R.; Goettelman, R.

1974-01-01

Two methods are used to estimate soil moisture remotely using the 0.4- to 14.0-micron wavelength region: (1) measurement of spectral reflectance, and (2) measurement of soil temperature. The reflectance method is based on observations which show that directional reflectance decreases as soil moisture increases for a given material. The soil temperature method is based on observations which show that differences between daytime and nighttime soil temperatures decrease as moisture content increases for a given material. In some circumstances, separate reflectance or temperature measurements yield ambiguous data, in which case these two methods may be combined to obtain a valid soil moisture determination. In this combined approach, reflectance is used to estimate low moisture levels; and thermal inertia (or thermal diffusivity) is used to estimate higher levels. The reflectance method appears promising for surface estimates of soil moisture, whereas the temperature method appears promising for estimates of near-subsurface (0 to 10 cm).

Establishing the diffuse correlation spectroscopy signal relationship with blood flow.

PubMed

Boas, David A; Sakadžić, Sava; Selb, Juliette; Farzam, Parisa; Franceschini, Maria Angela; Carp, Stefan A

2016-07-01

Diffuse correlation spectroscopy (DCS) measurements of blood flow rely on the sensitivity of the temporal autocorrelation function of diffusively scattered light to red blood cell (RBC) mean square displacement (MSD). For RBCs flowing with convective velocity [Formula: see text], the autocorrelation is expected to decay exponentially with [Formula: see text], where [Formula: see text] is the delay time. RBCs also experience shear-induced diffusion with a diffusion coefficient [Formula: see text] and an MSD of [Formula: see text]. Surprisingly, experimental data primarily reflect diffusive behavior. To provide quantitative estimates of the relative contributions of convective and diffusive movements, we performed Monte Carlo simulations of light scattering through tissue of varying vessel densities. We assumed laminar vessel flow profiles and accounted for shear-induced diffusion effects. In agreement with experimental data, we found that diffusive motion dominates the correlation decay for typical DCS measurement parameters. Furthermore, our model offers a quantitative relationship between the RBC diffusion coefficient and absolute tissue blood flow. We thus offer, for the first time, theoretical support for the empirically accepted ability of the DCS blood flow index ([Formula: see text]) to quantify tissue perfusion. We find [Formula: see text] to be linearly proportional to blood flow, but with a proportionality modulated by the hemoglobin concentration and the average blood vessel diameter.

ADEOS Total Ozone Mapping Spectrometer (TOMS) Data Products User's Guide

NASA Technical Reports Server (NTRS)

Krueger, A.; Bhartia, P. K.; McPeters, R.; Herman, J.; Wellemeyer, C.; Jaross, G.; Seftor, C.; Torres, O.; Labow, G.; Byerly, W.;

Earth Probe Total Ozone Mapping Spectrometer (TOMS) Data Product User's Guide

NASA Technical Reports Server (NTRS)

McPeters, R.; Bhartia, P. K.; Krueger, A.; Herman, J.; Wellemeyer, C.; Seftor, C.; Jaross, G.; Torres, O.; Moy, L.; Labow, G.;

Estimation of biomedical optical properties by simultaneous use of diffuse reflectometry and photothermal radiometry: investigation of light propagation models

NASA Astrophysics Data System (ADS)

Fonseca, E. S. R.; de Jesus, M. E. P.

2007-07-01

The estimation of optical properties of highly turbid and opaque biological tissue is a difficult task since conventional purely optical methods rapidly loose sensitivity as the mean photon path length decreases. Photothermal methods, such as pulsed or frequency domain photothermal radiometry (FD-PTR), on the other hand, show remarkable sensitivity in experimental conditions that produce very feeble optical signals. Photothermal Radiometry is primarily sensitive to absorption coefficient yielding considerably higher estimation errors on scattering coefficients. Conversely, purely optical methods such as Local Diffuse Reflectance (LDR) depend mainly on the scattering coefficient and yield much better estimates of this parameter. Therefore, at moderate transport albedos, the combination of photothermal and reflectance methods can improve considerably the sensitivity of detection of tissue optical properties. The authors have recently proposed a novel method that combines FD-PTR with LDR, aimed at improving sensitivity on the determination of both optical properties. Signal analysis was performed by global fitting the experimental data to forward models based on Monte-Carlo simulations. Although this approach is accurate, the associated computational burden often limits its use as a forward model. Therefore, the application of analytical models based on the diffusion approximation offers a faster alternative. In this work, we propose the calculation of the diffuse reflectance and the fluence rate profiles under the δ-P I approximation. This approach is known to approximate fluence rate expressions better close to collimated sources and boundaries than the standard diffusion approximation (SDA). We extend this study to the calculation of the diffuse reflectance profiles. The ability of the δ-P I based model to provide good estimates of the absorption, scattering and anisotropy coefficients is tested against Monte-Carlo simulations over a wide range of scattering to absorption ratios. Experimental validation of the proposed method is accomplished by a set of measurements on solid absorbing and scattering phantoms.

Polarization impacts on the water-leaving radiance retrieval from above-water radiometric measurements.

PubMed

Harmel, Tristan; Gilerson, Alexander; Tonizzo, Alberto; Chowdhary, Jacek; Weidemann, Alan; Arnone, Robert; Ahmed, Sam

2012-12-10

Above-water measurements of water-leaving radiance are widely used for water-quality monitoring and ocean-color satellite data validation. Reflected skylight in above-water radiometry needs to be accurately estimated prior to derivation of water-leaving radiance. Up-to-date methods to estimate reflection of diffuse skylight on rough sea surfaces are based on radiative transfer simulations and sky radiance measurements. But these methods neglect the polarization state of the incident skylight, which is generally highly polarized. In this paper, the effects of polarization on the sea surface reflectance and the subsequent water-leaving radiance estimation are investigated. We show that knowledge of the polarization field of the diffuse skylight significantly improves above-water radiometry estimates, in particular in the blue part of the spectrum where the reflected skylight is dominant. A newly developed algorithm based on radiative transfer simulations including polarization is described. Its application to the standard Aerosol Robotic Network-Ocean Color and hyperspectral radiometric measurements of the 1.5-year dataset acquired at the Long Island Sound site demonstrates the noticeable importance of considering polarization for water-leaving radiance estimation. In particular it is shown, based on time series of collocated data acquired in coastal waters, that the azimuth range of measurements leading to good-quality data is significantly increased, and that these estimates are improved by more than 12% at 413 nm. Full consideration of polarization effects is expected to significantly improve the quality of the field data utilized for satellite data validation or potential vicarious calibration purposes.

Structured light optical microscopy for three-dimensional reconstruction of technical surfaces

NASA Astrophysics Data System (ADS)

Kettel, Johannes; Reinecke, Holger; Müller, Claas

2016-04-01

In microsystems technology quality control of micro structured surfaces with different surface properties is playing an ever more important role. The process of quality control incorporates three-dimensional (3D) reconstruction of specularand diffusive reflecting technical surfaces. Due to the demand on high measurement accuracy and data acquisition rates, structured light optical microscopy has become a valuable solution to solve this problem providing high vertical and lateral resolution. However, 3D reconstruction of specular reflecting technical surfaces still remains a challenge to optical measurement principles. In this paper we present a measurement principle based on structured light optical microscopy which enables 3D reconstruction of specular- and diffusive reflecting technical surfaces. It is realized using two light paths of a stereo microscope equipped with different magnification levels. The right optical path of the stereo microscope is used to project structured light onto the object surface. The left optical path is used to capture the structured illuminated object surface with a camera. Structured light patterns are generated by a Digital Light Processing (DLP) device in combination with a high power Light Emitting Diode (LED). Structured light patterns are realized as a matrix of discrete light spots to illuminate defined areas on the object surface. The introduced measurement principle is based on multiple and parallel processed point measurements. Analysis of the measured Point Spread Function (PSF) by pattern recognition and model fitting algorithms enables the precise calculation of 3D coordinates. Using exemplary technical surfaces we demonstrate the successful application of our measurement principle.

MODIS Solar Diffuser: Modelled and Actual Performance

NASA Technical Reports Server (NTRS)

Waluschka, Eugene; Xiong, Xiao-Xiong; Esposito, Joe; Wang, Xin-Dong; Krebs, Carolyn (Technical Monitor)

2001-01-01

The Moderate Resolution Imaging Spectroradiometer (MODIS) instrument's solar diffuser is used in its radiometric calibration for the reflective solar bands (VIS, NTR, and SWIR) ranging from 0.41 to 2.1 micron. The sun illuminates the solar diffuser either directly or through a attenuation screen. The attenuation screen consists of a regular array of pin holes. The attenuated illumination pattern on the solar diffuser is not uniform, but consists of a multitude of pin-hole images of the sun. This non-uniform illumination produces small, but noticeable radiometric effects. A description of the computer model used to simulate the effects of the attenuation screen is given and the predictions of the model are compared with actual, on-orbit, calibration measurements.

Non-contact assessment of COD and turbidity concentrations in water using diffuse reflectance UV-Vis spectroscopy.

PubMed

Agustsson, Jon; Akermann, Oliver; Barry, D Andrew; Rossi, Luca

2014-08-01

Water contamination is an important environmental concern underlining the need for reliable real-time information on contaminant concentrations in natural waters. Here, a new non-contact UV-Vis spectroscopic approach for monitoring contaminants in water, and especially wastewater, is proposed. Diffuse reflectance UV-Vis spectroscopy was applied to measure simultaneously the chemical oxygen demand (COD) and turbidity (TUR) concentrations in water. The measurements were carried out in the wavelength range from 200-1100 nm. The measured spectra were analysed using partial-least-squares (PLS) regression. The correlation coefficient between the measured and the reference concentrations of COD and TUR in the water samples were R(2) = 0.85 and 0.96, respectively. These results highlight the potential of non-contact UV-Vis spectroscopy for the assessment of water contamination. A system built on the concept would be able to monitor wastewater pollution continuously, without the need for laborious sample collection and subsequent laboratory analysis. Furthermore, since no parts of the system are in contact with the wastewater stream the need for maintenance is minimised.

Free water determines diffusion alterations and clinical status in cerebral small vessel disease.

PubMed

Duering, Marco; Finsterwalder, Sofia; Baykara, Ebru; Tuladhar, Anil Man; Gesierich, Benno; Konieczny, Marek J; Malik, Rainer; Franzmeier, Nicolai; Ewers, Michael; Jouvent, Eric; Biessels, Geert Jan; Schmidt, Reinhold; de Leeuw, Frank-Erik; Pasternak, Ofer; Dichgans, Martin

2018-06-01

Diffusion tensor imaging detects early tissue alterations in Alzheimer's disease and cerebral small vessel disease (SVD). However, the origin of diffusion alterations in SVD is largely unknown. To gain further insight, we applied free water (FW) imaging to patients with genetically defined SVD (Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy [CADASIL], n = 57), sporadic SVD (n = 444), and healthy controls (n = 28). We modeled freely diffusing water in the extracellular space (FW) and measures reflecting fiber structure (tissue compartment). We tested associations between these measures and clinical status (processing speed and disability). Diffusion alterations in SVD were mostly driven by increased FW and less by tissue compartment alterations. Among imaging markers, FW showed the strongest association with clinical status (R 2 up to 34%, P < .0001). Findings were consistent across patients with CADASIL and sporadic SVD. Diffusion alterations and clinical status in SVD are largely determined by extracellular fluid increase rather than alterations of white matter fiber organization. Copyright © 2018 the Alzheimer's Association. All rights reserved.

Neurite density from magnetic resonance diffusion measurements at ultrahigh field: Comparison with light microscopy and electron microscopy

PubMed Central

Jespersen, Sune N.; Bjarkam, Carsten R.; Nyengaard, Jens R.; Chakravarty, M. Mallar; Hansen, Brian; Vosegaard, Thomas; Østergaard, Leif; Yablonskiy, Dmitriy; Nielsen, Niels Chr.; Vestergaard-Poulsen, Peter

2010-01-01

Due to its unique sensitivity to tissue microstructure, diffusion-weighted magnetic resonance imaging (MRI) has found many applications in clinical and fundamental science. With few exceptions, a more precise correspondence between physiological or biophysical properties and the obtained diffusion parameters remain uncertain due to lack of specificity. In this work, we address this problem by comparing diffusion parameters of a recently introduced model for water diffusion in brain matter to light microscopy and quantitative electron microscopy. Specifically, we compare diffusion model predictions of neurite density in rats to optical myelin staining intensity and stereological estimation of neurite volume fraction using electron microscopy. We find that the diffusion model describes data better and that its parameters show stronger correlation with optical and electron microscopy, and thus reflect myelinated neurite density better than the more frequently used diffusion tensor imaging (DTI) and cumulant expansion methods. Furthermore, the estimated neurite orientations capture dendritic architecture more faithfully than DTI diffusion ellipsoids. PMID:19732836

Modeling Suomi-NPP VIIRS Solar Diffuser Degradation due to Space Radiation

NASA Astrophysics Data System (ADS)

Shao, X.; Cao, C.

2014-12-01

The Visible Infrared Imaging Radiometer Suite (VIIRS) onboard Suomi-NPP uses a solar diffuser (SD) as on-board radiometric calibrator for the reflective solar band (RSB) calibration. Solar diffuser is made of Spectralon (one type of fluoropolymer) and was chosen because of its controlled reflectance in the VIS-NIR-SWIR region and its near-Lambertian reflectance profile. Spectralon is known to degrade in reflectance at the blue end of the spectrum due to exposure to space radiations such as solar UV radiation and energetic protons. These space radiations can modify the Spectralon surface through breaking C-C and C-F bonds and scissioning or cross linking the polymer, which causes the surface roughness and degrades its reflectance. VIIRS uses a SDSM (Solar Diffuser Stability Monitor) to monitor the change in the Solar Diffuser reflectance in the 0.4 - 0.94 um wavelength range and provide a correction to the calibration constants. The H factor derived from SDSM reveals that reflectance of 0.4 to 0.6um channels of VIIRS degrades faster than the reflectance of longer wavelength RSB channels. A model is developed to derive characteristic parameters such as mean SD surface roughness height and autocovariance length of SD surface roughness from the long term spectral degradation of SD reflectance as monitored by SDSM. These two parameters are trended to assess development of surface roughness of the SD over the operation period of VIIRS.

A preliminary verification of the floating reference measurement method for non-invasive blood glucose sensing

NASA Astrophysics Data System (ADS)

Min, Xiaolin; Liu, Rong; Fu, Bo; Xu, Kexin

2017-06-01

In the non-invasive sensing of blood glucose by near-infrared diffuse reflectance spectroscopy, the spectrum is highly susceptible to the unstable and complicated background variations from the human body and the environment. In in vitro analyses, background variations are usually corrected by the spectrum of a standard reference sample that has similar optical properties to the analyte of interest. However, it is hard to find a standard sample for the in vivo measurement. Therefore, the floating reference measurement method is proposed to enable relative measurements in vivo, where the spectra under some special source-detector distance, defined as the floating reference position, are insensitive to the changes in glucose concentration due to the absorption effect and scattering effect. Because the diffuse reflectance signals at the floating reference positions only reflect the information on background variations during the measurement, they can be used as the internal reference. In this paper, the theoretical basis of the floating reference positions in a semi-infinite turbid medium was discussed based on the steady-state diffusion equation and its analytical solutions in a semi-infinite turbid medium (under the extrapolated boundary conditions). Then, Monte-Carlo (MC) simulations and in vitro experiments based on a custom-built continuous-moving spatially resolving double-fiber NIR measurement system, configured with two types of light source, a super luminescent diode (SLD) and a super-continuum laser, were carried out to verify the existence of the floating reference position in 5%, 10% and 20% Intralipid solutions. The results showed that the simulation values of the floating reference positions are close to the theoretical results, with a maximum deviation of approximately 0.3 mm in 1100-1320 nm. Great differences can be observed in 1340-1400 nm because the optical properties of Intralipid in this region don not satisfy the conditions of the steady-state diffusion equation. For the in vitro experiments, floating reference positions exist in 1220 nm and 1320 nm under two types of light source, and the results are quite close. However, the reference positions obtained from experiments are further from the light source compared with those obtained in the MC simulation. For the turbid media and the wavelengths investigated, the difference is up to 1 mm. This study is important for the design of optical fibers to be applied in the floating reference measurement.

Diffusion Tensor Magnetic Resonance Imaging Strategies for Color Mapping of Human Brain Anatomy

PubMed Central

Boujraf, Saïd

2018-01-01

Background: A color mapping of fiber tract orientation using diffusion tensor imaging (DTI) can be prominent in clinical practice. The goal of this paper is to perform a comparative study of visualized diffusion anisotropy in the human brain anatomical entities using three different color-mapping techniques based on diffusion-weighted imaging (DWI) and DTI. Methods: The first technique is based on calculating a color map from DWIs measured in three perpendicular directions. The second technique is based on eigenvalues derived from the diffusion tensor. The last technique is based on three eigenvectors corresponding to sorted eigenvalues derived from the diffusion tensor. All magnetic resonance imaging measurements were achieved using a 1.5 Tesla Siemens Vision whole body imaging system. A single-shot DW echoplanar imaging sequence used a Stejskal–Tanner approach. Trapezoidal diffusion gradients are used. The slice orientation was transverse. The basic measurement yielded a set of 13 images. Each series consists of a single image without diffusion weighting, besides two DWIs for each of the next six noncollinear magnetic field gradient directions. Results: The three types of color maps were calculated consequently using the DWI obtained and the DTI. Indeed, we established an excellent similarity between the image data in the color maps and the fiber directions of known anatomical structures (e.g., corpus callosum and gray matter). Conclusions: In the meantime, rotationally invariant quantities such as the eigenvectors of the diffusion tensor reflected better, the real orientation found in the studied tissue. PMID:29928631

Analysis of hemodynamics in human skin using photothermal radiometry and diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Verdel, Nina; Marin, Ana; Vidovič, Luka; Milanič, Matija; Majaron, Boris

2017-07-01

We present a novel methodology for quantitative analysis of hemodynamics in human skin in vivo. Our approach combines pulsed photothermal radiometry (i.e., time-resolved measurements of midinfrared emission from sample surface after exposure to a short light pulse) and diffuse reflectance spectroscopy in visible part of the spectrum. Experimental data are fitted with predictions of a numerical model of light transport in a four-layer skin model (i.e., inverse Monte Carlo), which allows assessment of the layer thicknesses, chromophore contents (e.g., melanin, oxy- and deoxy-hemoglobin), as well as scattering properties. The performance is tested in comparison analysis of healthy skin before and during application of a blood pressure cuff (at 200 mm Hg) for 5 minutes.

Experimental analysis of bruises in human volunteers using radiometric depth profiling and diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Vidovič, Luka; Milanič, Matija; Majaron, Boris

2015-07-01

We combine pulsed photothermal radiometry (PPTR) depth profiling with diffuse reflectance spectroscopy (DRS) measurements for a comprehensive analysis of bruise evolution in vivo. While PPTR enables extraction of detailed depth distribution and concentration profiles of selected absorbers (e.g. melanin, hemoglobin), DRS provides information in a wide range of visible wavelengths and thus offers an additional insight into dynamics of the hemoglobin degradation products. Combining the two approaches enables us to quantitatively characterize bruise evolution dynamics. Our results indicate temporal variations of the bruise evolution parameters in the course of bruise self-healing process. The obtained parameter values and trends represent a basis for a future development of an objective technique for bruise age determination.

Time-resolved measurements in diffuse reflectance: effects of the instrument response function of different detection systems on the depth sensitivity

NASA Astrophysics Data System (ADS)

Puszka, Agathe; Planat-Chrétien, Anne; Berger, Michel; Hervé, Lionel; Dinten, Jean-Marc

2014-02-01

We demonstrate the loss of depth sensitivity induced by the instrument response function on reflectance time-resolved diffuse optical tomography through the comparison of 3 detection systems: on one hand a photomultiplier tube (PMT) and a hybrid PMT coupled with a time-correlated single-photon counting card and on the other hand a high rate intensified camera. We experimentally evaluate the depth sensitivity achieved for each detection module with an absorbing inclusion embedded in a turbid medium. The different interfiber distances of 5, 10 and 15 mm are considered. Finally, we determine a maximal depth reached for each detection system by using 3D tomographic reconstructions based on the Mellin-Laplace transform.

Explicit validation of a surface shortwave radiation balance model over snow-covered complex terrain

NASA Astrophysics Data System (ADS)

Helbig, N.; Löwe, H.; Mayer, B.; Lehning, M.

2010-09-01

A model that computes the surface radiation balance for all sky conditions in complex terrain is presented. The spatial distribution of direct and diffuse sky radiation is determined from observations of incident global radiation, air temperature, and relative humidity at a single measurement location. Incident radiation under cloudless sky is spatially derived from a parameterization of the atmospheric transmittance. Direct and diffuse sky radiation for all sky conditions are obtained by decomposing the measured global radiation value. Spatial incident radiation values under all atmospheric conditions are computed by adjusting the spatial radiation values obtained from the parametric model with the radiation components obtained from the decomposition model at the measurement site. Topographic influences such as shading are accounted for. The radiosity approach is used to compute anisotropic terrain reflected radiation. Validations of the shortwave radiation balance model are presented in detail for a day with cloudless sky. For a day with overcast sky a first validation is presented. Validation of a section of the horizon line as well as of individual radiation components is performed with high-quality measurements. A new measurement setup was designed to determine terrain reflected radiation. There is good agreement between the measurements and the modeled terrain reflected radiation values as well as with incident radiation values. A comparison of the model with a fully three-dimensional radiative transfer Monte Carlo model is presented. That validation reveals a good agreement between modeled radiation values.

A Method for Medical Diagnosis Based on Optical Fluence Rate Distribution at Tissue Surface

PubMed Central

Hamdy, Omnia; El-Azab, Jala; Al-Saeed, Tarek A.; Hassan, Mahmoud F.

2017-01-01

Optical differentiation is a promising tool in biomedical diagnosis mainly because of its safety. The optical parameters’ values of biological tissues differ according to the histopathology of the tissue and hence could be used for differentiation. The optical fluence rate distribution on tissue boundaries depends on the optical parameters. So, providing image displays of such distributions can provide a visual means of biomedical diagnosis. In this work, an experimental setup was implemented to measure the spatially-resolved steady state diffuse reflectance and transmittance of native and coagulated chicken liver and native and boiled breast chicken skin at 635 and 808 nm wavelengths laser irradiation. With the measured values, the optical parameters of the samples were calculated in vitro using a combination of modified Kubelka-Munk model and Bouguer-Beer-Lambert law. The estimated optical parameters values were substituted in the diffusion equation to simulate the fluence rate at the tissue surface using the finite element method. Results were verified with Monte-Carlo simulation. The results obtained showed that the diffuse reflectance curves and fluence rate distribution images can provide discrimination tools between different tissue types and hence can be used for biomedical diagnosis. PMID:28930158

A Method for Medical Diagnosis Based on Optical Fluence Rate Distribution at Tissue Surface.

PubMed

Hamdy, Omnia; El-Azab, Jala; Al-Saeed, Tarek A; Hassan, Mahmoud F; Solouma, Nahed H

2017-09-20

Optical differentiation is a promising tool in biomedical diagnosis mainly because of its safety. The optical parameters' values of biological tissues differ according to the histopathology of the tissue and hence could be used for differentiation. The optical fluence rate distribution on tissue boundaries depends on the optical parameters. So, providing image displays of such distributions can provide a visual means of biomedical diagnosis. In this work, an experimental setup was implemented to measure the spatially-resolved steady state diffuse reflectance and transmittance of native and coagulated chicken liver and native and boiled breast chicken skin at 635 and 808 nm wavelengths laser irradiation. With the measured values, the optical parameters of the samples were calculated in vitro using a combination of modified Kubelka-Munk model and Bouguer-Beer-Lambert law. The estimated optical parameters values were substituted in the diffusion equation to simulate the fluence rate at the tissue surface using the finite element method. Results were verified with Monte-Carlo simulation. The results obtained showed that the diffuse reflectance curves and fluence rate distribution images can provide discrimination tools between different tissue types and hence can be used for biomedical diagnosis.

Manifestation of two-channel nonlocal spin transport in the shapes of Hanle curves

NASA Astrophysics Data System (ADS)

Roundy, R. C.; Prestgard, M. C.; Tiwari, A.; Mishchenko, E. G.; Raikh, M. E.

2014-09-01

The dynamics of charge-density fluctuations in a system of two tunnel-coupled wires contains two diffusion modes with dispersion iω =Dq2 and iω =Dq2+2/τt, where D is the diffusion coefficient and τt is the tunneling time between the wires. The dispersion of corresponding spin-density modes depends on magnetic field as a result of the spin precession with Larmour frequency ωL. The presence of two modes affects the shape of the Hanle curve describing the spin-dependent resistance R between the ferromagnetic strips covering the nonmagnetic wires. We demonstrate that the relative shapes of the R (ωL) curves, one measured within the same wire and the other measured between the wires, depends on the ratio τt/τs, where τs is the spin-diffusion time. If the coupling between the wires is local, i.e., only at the point x =0, then the difference of the shapes of intrawire and interwire Hanle curves reflects the difference in statistics of diffusive trajectories, which "switch" or do not switch near x =0. When one of the coupled wires is bent into a loop with a radius a, the shape of the Hanle curve reflects the statistics of random walks on the loop. This statistics is governed by the dimensionless parameter a /√Dτs .

Multipath induced errors in meteorological Doppler/interferometer location systems

NASA Technical Reports Server (NTRS)

Wallace, R. G.

1984-01-01

One application of an RF interferometer aboard a low-orbiting spacecraft to determine the location of ground-based transmitters is in tracking high-altitude balloons for meteorological studies. A source of error in this application is reflection of the signal from the sea surface. Through propagating and signal analysis, the magnitude of the reflection-induced error in both Doppler frequency measurements and interferometer phase measurements was estimated. The theory of diffuse scattering from random surfaces was applied to obtain the power spectral density of the reflected signal. The processing of the combined direct and reflected signals was then analyzed to find the statistics of the measurement error. It was found that the error varies greatly during the satellite overpass and attains its maximum value at closest approach. The maximum values of interferometer phase error and Doppler frequency error found for the system configuration considered were comparable to thermal noise-induced error.

Optical inverse-square displacement sensor

DOEpatents

Howe, Robert D.; Kychakoff, George

1989-01-01

This invention comprises an optical displacement sensor that uses the inverse-square attenuation of light reflected from a diffused surface to calculate the distance from the sensor to the reflecting surface. Light emerging from an optical fiber or the like is directed onto the surface whose distance is to be measured. The intensity I of reflected light is angle dependent, but within a sufficiently small solid angle it falls off as the inverse square of the distance from the surface. At least a pair of optical detectors are mounted to detect the reflected light within the small solid angle, their ends being at different distances R and R+.DELTA.R from the surface. The distance R can then be found in terms of the ratio of the intensity measurements and the separation length as ##EQU1##

Assessment of MODIS and VIIRS Solar Diffuser On-Orbit Degradation

NASA Technical Reports Server (NTRS)

Xiong, Xiaoxiong; Fulbright, Jon; Angal, Amit; Wang, Zhipeng; Geng, Xu; Butler, Jim

2015-01-01

Both MODIS and VIIRS instruments use a solar diffuser (SD) for their reflective solar bands (RSB) on-orbit calibration. On-orbit changes in SD bi-directional reflectance factor (BRF) are tracked by a solar diffuser stability monitor (SDSM) using its alternate measurements of the sunlight reflected off the SD panel and direct sunlight through a fixed attenuation screen. The SDSM calibration data are collected by a number of filtered detectors, covering wavelengths from 0.41 to 0.94 micrometers. In this paper we describe briefly the Terra and Aqua MODIS and S-NPP VIIRS SDSM on-orbit operation and calibration activities and strategies, provide an overall assessment of their SDSM on-orbit performance, including wavelength-dependent changes in the SDSM detector responses and changes in their SD BRF, and discuss remaining challenging issues and their potential impact on RSB calibration quality. Due to different launch dates, operating configurations, and calibration frequencies, the Terra and Aqua MODIS and S-NPP VIIRS SD have experienced different amount of SD degradation. However, in general the shorter the wavelength, the larger is the SD on-orbit degradation. On the other hand, the larger changes in SDSM detector responses are observed at longer wavelengths in the near infrared (NIR).

Assessment of MODIS and VIIRS solar diffuser on-orbit degradation

NASA Astrophysics Data System (ADS)

Xiong, Xiaoxiong; Fulbright, Jon; Angal, Amit; Wang, Zhipeng; Geng, Xu; Butler, Jim

2015-09-01

Both MODIS and VIIRS instruments use a solar diffuser (SD) for their reflective solar bands (RSB) on-orbit calibration. On-orbit changes in SD bi-directional reflectance factor (BRF) are tracked by a solar diffuser stability monitor (SDSM) using its alternate measurements of the sunlight reflected off the SD panel and direct sunlight through a fixed attenuation screen. The SDSM calibration data are collected by a number of filtered detectors, covering wavelengths from 0.41 to 0.94μm. In this paper we describe briefly the Terra and Aqua MODIS and S-NPP VIIRS SDSM on-orbit operation and calibration activities and strategies, provide an overall assessment of their SDSM on-orbit performance, including wavelength-dependent changes in the SDSM detector responses and changes in their SD BRF, and discuss remaining challenging issues and their potential impact on RSB calibration quality. Due to different launch dates, operating configurations, and calibration frequencies, the Terra and Aqua MODIS and S-NPP VIIRS SD have experienced different amount of SD degradation. However, in general the shorter the wavelength, the larger is the SD on-orbit degradation. On the other hand, the larger changes in SDSM detector responses are observed at longer wavelengths in the near infrared (NIR).

Including scattering within the room acoustics diffusion model: An analytical approach.

PubMed

Foy, Cédric; Picaut, Judicaël; Valeau, Vincent

2016-10-01

Over the last 20 years, a statistical acoustic model has been developed to predict the reverberant sound field in buildings. This model is based on the assumption that the propagation of the reverberant sound field follows a transport process and, as an approximation, a diffusion process that can be easily solved numerically. This model, initially designed and validated for rooms with purely diffuse reflections, is extended in the present study to mixed reflections, with a proportion of specular and diffuse reflections defined by a scattering coefficient. The proposed mathematical developments lead to an analytical expression of the diffusion constant that is a function of the scattering coefficient, but also on the absorption coefficient of the walls. The results obtained with this extended diffusion model are then compared with the classical diffusion model, as well as with a sound particles tracing approach considering mixed wall reflections. The comparison shows a good agreement for long rooms with uniform low absorption (α = 0.01) and uniform scattering. For a larger absorption (α = 0.1), the agreement is moderate, due to the fact that the proposed expression of the diffusion coefficient does not vary spatially. In addition, the proposed model is for now limited to uniform diffusion and should be extended in the future to more general cases.

National Institute of Standards and Technology measurement service of the optical properties of biomedical phantoms: Current status.

PubMed

Lemaillet, Paul; Cooksey, Catherine C; Levine, Zachary H; Pintar, Adam L; Hwang, Jeeseong; Allen, David W

2016-03-24

The National Institute of Standards and Technology (NIST) has maintained scales for reflectance and transmittance over several decades. The scales are primarily intended for regular transmittance, mirrors, and solid surface scattering diffusers. The rapidly growing area of optical medical imaging needs a scale for volume scattering of diffuse materials that are used to mimic the optical properties of tissue. Such materials are used as phantoms to evaluate and validate instruments under development intended for clinical use. To address this need, a double-integrating sphere based instrument has been installed to measure the optical properties of tissue-mimicking phantoms. The basic system and methods have been described in previous papers. An important attribute in establishing a viable calibration service is the estimation of measurement uncertainties. The use of custom models and comparisons with other established scales enabled uncertainty measurements. Here, we describe the continuation of those efforts to advance the understanding of the uncertainties through two independent measurements: the bidirectional reflectance distribution function and the bidirectional transmittance distribution function of a commercially available solid biomedical phantom. A Monte Carlo-based model is used and the resulting optical properties are compared to the values provided by the phantom manufacturer.

Analysis of ecstasy tablets: comparison of reflectance and transmittance near infrared spectroscopy.

PubMed

Schneider, Ralph Carsten; Kovar, Karl-Artur

2003-07-08

Calibration models for the quantitation of commonly used ecstasy substances have been developed using near infrared spectroscopy (NIR) in diffuse reflectance and in transmission mode by applying seized ecstasy tablets for model building and validation. The samples contained amphetamine, N-methyl-3,4-methylenedioxy-amphetamine (MDMA) and N-ethyl-3,4-methylenedioxy-amphetamine (MDE) in different concentrations. All tablets were analyzed using high performance liquid chromatography (HPLC) with diode array detection as reference method. We evaluated the performance of each NIR measurement method with regard to its ability to predict the content of each tablet with a low root mean square error of prediction (RMSEP). Best calibration models could be generated by using NIR measurement in transmittance mode with wavelength selection and 1/x-transformation of the raw data. The models build in reflectance mode showed higher RMSEPs using as data pretreatment, wavelength selection, 1/x-transformation and a second order Savitzky-Golay derivative with five point smoothing was applied to obtain the best models. To estimate the influence of inhomogeneities in the illegal tablets, a calibration of the destroyed, i.e. triturated samples was build and compared to the corresponding data of the whole tablets. The calibrations using these homogenized tablets showed lower RMSEPs. We can conclude that NIR analysis of ecstasy tablets in transmission mode is more suitable than measurement in diffuse reflectance to obtain quantification models for their active ingredients with regard to low errors of prediction. Inhomogeneities in the samples are equalized when measuring the tablets as powdered samples.

In Vivo Monitoring of Hemodynamic Changes during Clogging and Unclogging of Blood Supply for the Application of Clinical Shock Detection

NASA Astrophysics Data System (ADS)

Kanawade, Rajesh; Stelzle, Florian; Schmidt, Michael

This paper presents a novel methodology in early detection of clinical shock by monitoring hemodynamic changes using diffuse reflectance measurement technique. Detailed prototype of the reflectance measurement system and data analysis technique of hemodynamic monitoring was carried out in our laboratory. The real time in-vivo measurements were done from the index finger. This study demonstrates preliminary results of real time monitoring of reduced/- oxyhemoglobin changes during clogging and unclogging of blood flow in the finger tip. The obtained results were verified with pulse-oximeter values, connected to the tip of the same index finger.

Lung diffusing capacity for nitric oxide as a marker of fibrotic changes in idiopathic interstitial pneumonias.

PubMed

Barisione, Giovanni; Brusasco, Claudia; Garlaschi, Alessandro; Baroffio, Michele; Brusasco, Vito

2016-05-01

Lung diffusing capacity for carbon monoxide (DLCO) is decreased in both usual interstitial pneumonia-idiopathic pulmonary fibrosis (UIP-IPF) and nonspecific interstitial pneumonia (NSIP), but is moderately related to computed tomography (CT)-determined fibrotic changes. This may be due to the relative insensitivity of DLCO to changes in alveolar membrane diffusive conductance (DMCO). The purpose of this study was to determine whether measurement of lung diffusing capacity for nitric oxide (DLNO) better reflects fibrotic changes than DLCO DLNO-DLCO were measured simultaneously in 30 patients with UIP-IPF and 30 with NSIP. Eighty-one matched healthy subjects served as a control group. The amount of pulmonary fibrosis was estimated by CT volumetric analysis of visually bounded areas showing reticular opacities and honeycombing. DMCO and pulmonary capillary volume (VC) were calculated. DLNO was below the lower limit of normal in all patients irrespective of extent and nature of disease, whereas DLCO was within the normal range in a nonnegligible number of patients. Both DLNO and DLCO were significantly correlated with visual assessment of fibrosis but DLNO more closely than DLCO DMCO was also below the lower limit of normal in all UIP-IPF and NSIP patients and significantly correlated with fibrosis extent in both diseases, whereas VC was weakly correlated with fibrosis in UIP-IPF and uncorrelated in NSIP, with normal values in half of patients. In conclusion, measurement of DLNO may provide a more sensitive evaluation of fibrotic changes than DLCO in either UIP-IPF or NSIP, because it better reflects DMCO. Copyright © 2016 the American Physiological Society.

Reflectance of polytetrafluoroethylene for xenon scintillation light

NASA Astrophysics Data System (ADS)

Silva, C.; Pinto da Cunha, J.; Pereira, A.; Chepel, V.; Lopes, M. I.; Solovov, V.; Neves, F.

2010-03-01

Gaseous and liquid xenon particle detectors are being used in a number of applications including dark matter search and neutrino-less double beta decay experiments. Polytetrafluoroethylene (PTFE) is often used in these detectors both as electrical insulator and as a light reflector to improve the efficiency of detection of scintillation photons. However, xenon emits in the vacuum ultraviolet (VUV) wavelength region (λ ≃175 nm) where the reflecting properties of PTFE are not sufficiently known. In this work, we report on measurements of PTFE reflectance, including its angular distribution, for the xenon scintillation light. Various samples of PTFE, manufactured by different processes (extruded, expanded, skived, and pressed) have been studied. The data were interpreted with a physical model comprising both specular and diffuse reflections. The reflectance obtained for these samples ranges from about 47% to 66% for VUV light. Other fluoropolymers, namely, ethylene tetrafluoroethylene (ETFE), fluorinated ethylene propylene (FEP), and perfluoro-alkoxyalkane (PFA) were also measured.

Broadband diffuse terahertz wave scattering by flexible metasurface with randomized phase distribution

PubMed Central

Zhang, Yin; Liang, Lanju; Yang, Jing; Feng, Yijun; Zhu, Bo; Zhao, Junming; Jiang, Tian; Jin, Biaobing; Liu, Weiwei

2016-01-01

Suppressing specular electromagnetic wave reflection or backward radar cross section is important and of broad interests in practical electromagnetic engineering. Here, we present a scheme to achieve broadband backward scattering reduction through diffuse terahertz wave reflection by a flexible metasurface. The diffuse scattering of terahertz wave is caused by the randomized reflection phase distribution on the metasurface, which consists of meta-particles of differently sized metallic patches arranged on top of a grounded polyimide substrate simply through a certain computer generated pseudorandom sequence. Both numerical simulations and experimental results demonstrate the ultralow specular reflection over a broad frequency band and wide angle of incidence due to the re-distribution of the incident energy into various directions. The diffuse scattering property is also polarization insensitive and can be well preserved when the flexible metasurface is conformably wrapped on a curved reflective object. The proposed design opens up a new route for specular reflection suppression, and may be applicable in stealth and other technology in the terahertz spectrum. PMID:27225031

Broadband diffuse terahertz wave scattering by flexible metasurface with randomized phase distribution.

PubMed

Zhang, Yin; Liang, Lanju; Yang, Jing; Feng, Yijun; Zhu, Bo; Zhao, Junming; Jiang, Tian; Jin, Biaobing; Liu, Weiwei

2016-05-26

Suppressing specular electromagnetic wave reflection or backward radar cross section is important and of broad interests in practical electromagnetic engineering. Here, we present a scheme to achieve broadband backward scattering reduction through diffuse terahertz wave reflection by a flexible metasurface. The diffuse scattering of terahertz wave is caused by the randomized reflection phase distribution on the metasurface, which consists of meta-particles of differently sized metallic patches arranged on top of a grounded polyimide substrate simply through a certain computer generated pseudorandom sequence. Both numerical simulations and experimental results demonstrate the ultralow specular reflection over a broad frequency band and wide angle of incidence due to the re-distribution of the incident energy into various directions. The diffuse scattering property is also polarization insensitive and can be well preserved when the flexible metasurface is conformably wrapped on a curved reflective object. The proposed design opens up a new route for specular reflection suppression, and may be applicable in stealth and other technology in the terahertz spectrum.

Mode-converted diffuse ultrasonic backscatter.

PubMed

Hu, Ping; Kube, Christopher M; Koester, Lucas W; Turner, Joseph A

2013-08-01

Diffuse ultrasonic backscatter describes the scattering of elastic waves from interfaces within heterogeneous materials. Previously, theoretical models have been developed for the diffuse backscatter of longitudinal-to-longitudinal (L-L) wave scattering within polycrystalline materials. Following a similar formalism, a mode-conversion scattering model is presented here to quantify the component of an incident longitudinal wave that scatters and is converted to a transverse (shear) wave within a polycrystalline sample. The model is then used to fit experimental measurements associated with a pitch-catch transducer configuration performed using a sample of 1040 steel. From these measurements, an average material correlation length is determined. This value is found to be in agreement with results from L-L scattering measurements and is on the order of the grain size as determined from optical micrographs. Mode-converted ultrasonic backscatter is influenced much less by the front-wall reflection than an L-L measurement and it provides additional microstructural information that is not accessible in any other manner.

Light scattering properties of new materials for glazing applications

NASA Astrophysics Data System (ADS)

Bergkvist, Mikael; Roos, Arne

1991-12-01

Several new materials are available for glazing applications, many of which require careful optical characterization, especially with regards to light scattering. Measuring scattering requires special equipment and is inherently difficult. An integrating sphere can be used for the total and diffuse components but great care must be taken in interpreting the instrument readings. Angular resolved scattering measurements are necessary for a complete characterization, and this is difficult for low levels of scattering. In this paper, measurements on electrically switchable NCAP materials and thick panes of aerogel are reported. The NCAP films switch reversibly from a translucent, scattering state to a transparent, clear state with the application of an ac-voltage. Airglass has a porous SiO2 structure with a refractive index n equals 1.04 and a very low heat transfer coefficient. Integrated scattering measurements were performed in the wavelength range 300 to 2500 nm on a Beckman 5240 spectrophotometer equipped with a 198851 integrating sphere. In this instrument we can measure the total and diffuse components of the reflectance or transmittance separately. The angular distribution of the scattered light was measured in a scatterometer, which can perform scattering measurements in the wavelength range 400-1100 nm in both transmittance and reflectance mode with variable angle of incidence.

Creation and Validation of Sintered PTFE BRDF Targets & Standards

PubMed Central

Durell, Christopher; Scharpf, Dan; McKee, Greg; L’Heureux, Michelle; Georgiev, Georgi; Obein, Gael; Cooksey, Catherine

2016-01-01

Sintered polytetrafluoroethylene (PTFE) is an extremely stable, near-perfect Lambertian reflecting diffuser and calibration standard material that has been used by national labs, space, aerospace and commercial sectors for over two decades. New uncertainty targets of 2 % on-orbit absolute validation in the Earth Observing Systems community have challenged the industry to improve is characterization and knowledge of almost every aspect of radiometric performance (space and ground). Assuming “near perfect” reflectance for angular dependent measurements is no longer going to suffice for many program needs. The total hemispherical spectral reflectance provides a good mark of general performance; but, without the angular characterization of bidirectional reflectance distribution function (BRDF) measurements, critical data is missing from many applications and uncertainty budgets. Therefore, traceable BRDF measurement capability is needed to characterize sintered PTFE’s angular response and provide a full uncertainty profile to users. This paper presents preliminary comparison measurements of the BRDF of sintered PTFE from several laboratories to better quantify the BRDF of sintered PTFE, assess the BRDF measurement comparability between laboratories, and improve estimates of measurement uncertainties under laboratory conditions. PMID:26900206

Creation and Validation of Sintered PTFE BRDF Targets & Standards.

PubMed

Durell, Christopher; Scharpf, Dan; McKee, Greg; L'Heureux, Michelle; Georgiev, Georgi; Obein, Gael; Cooksey, Catherine

2015-09-21

Sintered polytetrafluoroethylene (PTFE) is an extremely stable, near-perfect Lambertian reflecting diffuser and calibration standard material that has been used by national labs, space, aerospace and commercial sectors for over two decades. New uncertainty targets of 2 % on-orbit absolute validation in the Earth Observing Systems community have challenged the industry to improve is characterization and knowledge of almost every aspect of radiometric performance (space and ground). Assuming "near perfect" reflectance for angular dependent measurements is no longer going to suffice for many program needs. The total hemispherical spectral reflectance provides a good mark of general performance; but, without the angular characterization of bidirectional reflectance distribution function (BRDF) measurements, critical data is missing from many applications and uncertainty budgets. Therefore, traceable BRDF measurement capability is needed to characterize sintered PTFE's angular response and provide a full uncertainty profile to users. This paper presents preliminary comparison measurements of the BRDF of sintered PTFE from several laboratories to better quantify the BRDF of sintered PTFE, assess the BRDF measurement comparability between laboratories, and improve estimates of measurement uncertainties under laboratory conditions.

'Combined reflectance stratigraphy' - subdivision of loess successions by diffuse reflectance spectrometry (DRS)

NASA Astrophysics Data System (ADS)

Szeberényi, Jozsef; Bradak-Hayashi, Balázs; Kiss, Klaudia; Kovács, József; Varga, György; Balázs, Réka; Szalai, Zoltán; Viczián, István

2016-04-01

The different varieties of loess (and intercalated paleosol layers) together constitute one of the most widespread terrestrial sediments, which was deposited, altered, and redeposited in the course of the changing climatic conditions of the Pleistocene. To reveal more information about Pleistocene climate cycles and/or environments the detailed lithostratigraphical subdivision and classification of the loess variations and paleosols are necessary. Beside the numerous method such as various field measurements, semi-quantitative tests and laboratory investigations, diffuse reflectance spectroscopy (DRS) is one of the well applied method on loess/paleosol sequences. Generally, DRS has been used to separate the detrital and pedogenic mineral component of the loess sections by the hematite/goethite ratio. DRS also has been applied as a joint method of various environmental magnetic investigations such as magnetic susceptibility- and isothermal remanent magnetization measurements. In our study the so-called "combined reflectance stratigraphy method" were developed. At First, complex mathematical method was applied to compare the results of the spectral reflectance measurements. One of the most preferred multivariate methods is cluster analysis. Its scope is to group and compare the loess variations and paleosol based on the similarity and common properties of their reflectance curves. In the Second, beside the basic subdivision of the profiles by the different reflectance curves of the layers, the most characteristic wavelength section of the reflectance curve was determined. This sections played the most important role during the classification of the different materials of the section. The reflectance value of individual samples, belonged to the characteristic wavelength were depicted in the function of depth and well correlated with other proxies like grain size distribution and magnetic susceptibility data. The results of the correlation showed the significance of the "combined reflectance stratigraphy" as a stratigraphical method and as an environmental proxy also. Acknowledgment Bradák-Hayashi, B.'s fellowship at Department of Planetology (Kobe University, Japan) was supported by the Japan Society for the Promotion of Science (JSPS). The investigation was supported by International Visegrad Fund. Project No: 11410020.

Intraparticle diffusion limitations in the hydrogenation of monounsaturated edible oils and their fatty acid methyl esters

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jonker, G.H.; Veldsink, J.W.; Beenackers, A.A.C.M.

1998-12-01

Intraparticle diffusion limitation in the hydrogenation and isomerization of fatty acid methyl esters (FAMEs) and edible oils (triacylglycerol, TAG) in porous nickel catalyst was investigated both under reactive and under inert conditions. Under reactive conditions, the diffusion coefficients were determined from the best fits of the model simulations applying the intrinsic reacting kinetics of monounsaturated FAME hydrogenation to experiments under diffusion limited conditions. Due to the absence of reaction (hydrogenation of double bonds), the obtained effective H{sub z} diffusion coefficient (D{sub e}) with the HPLC technique is volume averaged and thereby determined by the larger intercrystalline pores (<30% of themore » total pore volume) only. Moreover, D{sub e} measured under reaction conditions reflected the influence of the micropores, resulting in a 10-fold lower value.« less

Ultra-broadband and planar sound diffuser with high uniformity of reflected intensity

NASA Astrophysics Data System (ADS)

Fan, Xu-Dong; Zhu, Yi-Fan; Liang, Bin; Yang, Jing; Yang, Jun; Cheng, Jian-Chun

2017-09-01

Schroeder diffusers, as a classical design of acoustic diffusers proposed over 40 years ago, play key roles in many practical scenarios ranging from architectural acoustics to noise control to particle manipulation. Despite the great success of conventional acoustic diffusers, it is still worth pursuing ideal acoustic diffusers that are essentially expected to produce perfect sound diffuse reflection within the unlimited bandwidth. Here, we propose a different mechanism for designing acoustic diffusers to overcome the basic limits in intensity uniformity and working bandwidth in the previous designs and demonstrate a practical implementation by acoustic metamaterials with dispersionless phase-steering capability. In stark contrast to the existing production of diffuse fields relying on random scattering of sound energy by using a specific mathematical number sequence of periodically distributed unit cells, we directly mold the reflected wavefront into the desired shape by precisely manipulating the local phases of individual subwavelength metastructures. We also benchmark our design via numerical simulation with a commercially available Schroeder diffuser, and the results verify that our proposed diffuser scatters incident acoustic energy into all directions more uniformly within an ultra-broad band regardless of the incident angle. Furthermore, our design enables further improvement of the working bandwidth just by simply downscaling each individual element. With ultra-broadband functionality and high uniformity of reflected intensity, our metamaterial-based production of the diffusive field opens a route to the design and application of acoustic diffusers and may have a significant impact on various fields such as architectural acoustics and medical ultrasound imaging/treatment.

Reference-free spectroscopic determination of fat and protein in milk in the visible and near infrared region below 1000nm using spatially resolved diffuse reflectance fiber probe.

PubMed

Bogomolov, Andrey; Belikova, Valeria; Galyanin, Vladislav; Melenteva, Anastasiia; Meyer, Hans

2017-05-15

New technique of diffuse reflectance spectroscopic analysis of milk fat and total protein content in the visible (Vis) and adjacent near infrared (NIR) region (400-995nm) has been developed and tested. Sample analysis was performed through a probe having eight 200-µm fiber channels forming a linear array. One of the end fibers was used for the illumination and other seven - for the spectroscopic detection of diffusely reflected light. One of the detection channels was used as a reference to normalize the spectra and to convert them into absorbance-equivalent units. The method has been tested experimentally using a designed sample set prepared from industrial raw milk standards with widely varying fat and protein content. To increase the modelling robustness all milk samples were measured in three different homogenization degrees. Comprehensive data analysis has shown the advantage of combining both spectral and spatial resolution in the same measurement and revealed the most relevant channels and wavelength regions. The modelling accuracy was further improved using joint variable selection and preprocessing optimization method based on the genetic algorithm. The root mean-square errors of different validation methods were below 0.10% for fat and below 0.08% for total protein content. Based on the present experimental data, it was computationally shown that the full-spectrum analysis in this method can be replaced by a sensor measurement at several specific wavelengths, for instance, using light-emitting diodes (LEDs) for illumination. Two optimal sensor configurations have been suggested: with nine LEDs for the analysis of fat and seven - for protein content. Both simulated sensors exhibit nearly the same component determination accuracy as corresponding full-spectrum analysis. Copyright © 2017 Elsevier B.V. All rights reserved.

In vivo determination of optical properties and fluorophore characteristics of non-melanoma skin cancer

NASA Astrophysics Data System (ADS)

Rajaram, Narasimhan; Kovacic, Dianne; Migden, Michael F.; Reichenberg, Jason S.; Nguyen, Tri H.; Tunnell, James W.

2009-02-01

Diffuse optical spectroscopy (DOS) and laser-induced fluorescence (LIF) techniques have widely been used as noninvasive tools for early cancer detection in several organs including the cervix, oral cavity and gastrointestinal tract. Using a combined DOS/LIF approach, one can simultaneously measure the morphology and biochemical composition of tissue and use these features to diagnose malignancy. We report for the first time to our knowledge both the optical properties and native fluorophore characteristics of non-melanoma skin cancer in the UV-visible range. We collected in vivo diffuse reflectance and intrinsic fluorescence measurements from 44 skin lesions on 37 patients. The skin sites were further categorized into three groups of non-melanoma skin cancer according to histopathology: 1) pre-cancerous actinic keratosis 2) malignant squamous cell carcinoma (SCC) and 3) basal cell carcinoma (BCC). We used a custom-built probe-based clinical system that collects both white light reflectance and laser-induced fluorescence in the wavelength range of 350-700 nm. We extracted the blood volume fraction, oxygen saturation, blood vessel size, tissue microarchitecture and melanin content from diffuse reflectance measurements. In addition, we determined the native fluorophore contributions of NADH, collagen and FAD from laser-induced fluorescence for all groups. The scattering from tissue decreased with progression from clinically normal to precancerous actinic keratosis to malignant SCC. A similar trend was observed for clinically normal skin and malignant BCC. Statistically significant differences were observed in the collagen contributions, which were lower in malignant SCC and BCC as compared to normal skin. Our data demonstrates that the mean optical properties and fluorophore contributions of normal, benign and malignant nonmelanoma cancers are significantly different from each other and can potentially be used as biomarkers for the early detection of skin cancer.

On-orbit solar calibrations using the Aqua Clouds and Earth's Radiant Energy System (CERES) in-flight calibration system

NASA Astrophysics Data System (ADS)

Wilson, Robert S.; Priestley, Kory J.; Thomas, Susan; Hess, Phillip

2009-08-01

The Clouds and the Earth's Radiant Energy System (CERES) spacecraft scanning thermistor bolometers were used to measure earth-reflected solar and earth-emitted longwave radiances, at satellite altitude. The bolometers measured the earth radiances in the broadband shortwave solar (0.3 - 5.0 micrometers) and total (0.3->100 micrometers) spectral bands as well as in the (8 - 12 micrometers) water vapor window spectral band over geographical footprints as small as 10 kilometers at nadir. In May 2002, the fourth and fifth sets of CERES bolometers were launched aboard the Aqua spacecraft. Ground vacuum calibrations defined the initial count conversion coefficients that were used to convert the bolometer output voltages into filtered earth radiances. The mirror attenuator mosaic (MAM), a solar diffuser plate, was built into the CERES instrument package calibration system in order to define in-orbit shifts or drifts in the sensor responses. The shortwave and total sensors are calibrated using the solar radiances reflected from the MAM's. Each MAM consists of baffle-solar diffuser plate systems, which guide incoming solar radiances into the instrument fields-of-view of the shortwave and total wave sensor units. The MAM diffuser reflecting type surface consists of an array of spherical aluminum mirror segments, which are separated by a Merck Black A absorbing surface, overcoated with silicon dioxide. Temperature sensors are located in each MAM plate and baffle. The CERES MAM wass designed to yield calibration precisions approaching .5 percent for the total and shortwave detectors. In this paper, the MAM solar calibration procedures are presented along with on-orbit results. Comparisons are also made between the Aqua,Terra and the Tropical Rainfall Measurement Mission (TRMM) CERES MAM solar calibrations.

A comparison of two laboratories for the measurement of wood dust using button sampler and diffuse reflection infrared Fourier-transform spectroscopy (DRIFTS).

PubMed

Chirila, Madalina M; Sarkisian, Khachatur; Andrew, Michael E; Kwon, Cheol-Woong; Rando, Roy J; Harper, Martin

2015-04-01

The current measurement method for occupational exposure to wood dust is by gravimetric analysis and is thus non-specific. In this work, diffuse reflection infrared Fourier transform spectroscopy (DRIFTS) for the analysis of only the wood component of dust was further evaluated by analysis of the same samples between two laboratories. Field samples were collected from six wood product factories using 25-mm glass fiber filters with the Button aerosol sampler. Gravimetric mass was determined in one laboratory by weighing the filters before and after aerosol collection. Diffuse reflection mid-infrared spectra were obtained from the wood dust on the filter which is placed on a motorized stage inside the spectrometer. The metric used for the DRIFTS analysis was the intensity of the carbonyl band in cellulose and hemicellulose at ~1735 cm(-1). Calibration curves were constructed separately in both laboratories using the same sets of prepared filters from the inhalable sampling fraction of red oak, southern yellow pine, and western red cedar in the range of 0.125-4 mg of wood dust. Using the same procedure in both laboratories to build the calibration curve and analyze the field samples, 62.3% of the samples measured within 25% of the average result with a mean difference between the laboratories of 18.5%. Some observations are included as to how the calibration and analysis can be improved. In particular, determining the wood type on each sample to allow matching to the most appropriate calibration increases the apparent proportion of wood dust in the sample and this likely provides more realistic DRIFTS results. Published by Oxford University Press on behalf of the British Occupational Hygiene Society 2014.

Results from Solar Reflective Band End-to-End Testing for VIIRS F1 Sensor Using T-SIRCUS

NASA Technical Reports Server (NTRS)

McIntire, Jeff; Moyer, David; McCarthy, James K.; DeLuccia, Frank; Xiong, Xiaoxiong; Butler, James J.; Guenther, Bruce

2011-01-01

Verification of the Visible Infrared Imager Radiometer Suite (VIIRS) End-to-End (E2E) sensor calibration is highly recommended before launch, to identify any anomalies and to improve our understanding of the sensor on-orbit calibration performance. E2E testing of the Reflective Solar Bands (RSB) calibration cycle was performed pre-launch for the VIIRS Fight 1 (F1) sensor at the Ball Aerospace facility in Boulder CO in March 2010. VIIRS reflective band calibration cycle is very similar to heritage sensor MODIS in that solar illumination, via a diffuser, is used to correct for temporal variations in the instrument responsivity. Monochromatic light from the NIST T-SIRCUS was used to illuminate both the Earth View (EV), via an integrating sphere, and the Solar Diffuser (SD) view, through a collimator. The collimator illumination was cycled through a series of angles intended to simulate the range of possible angles for which solar radiation will be incident on the solar attenuation screen on-orbit. Ideally, the measured instrument responsivity (defined here as the ratio of the detector response to the at-sensor radiance) should be the same whether the EV or SD view is illuminated. The ratio of the measured responsivities was determined at each collimator angle and wavelength. In addition, the Solar Diffuser Stability Monitor (SDSM), a ratioing radiometer designed to track the temporal variation in the SD BRF by direct comparison to solar radiation, was illuminated by the collimator. The measured SDSM ratio was compared to the predicted ratio. An uncertainty analysis was also performed on both the SD and SDSM calibrations.

On mimicking diffuse reflectance spectra in the visible and near-infrared ranges for tissue-like phantom design

NASA Astrophysics Data System (ADS)

Debernardi, N.; Dunias, P.; van El, B.; Statham, A. E.

2014-03-01

A novel methodology is presented to mimic diffuse reflectance spectra of arbitrary biological tissues in the visible and near-infrared ranges. The prerequisite for this method is that the spectral information of basic components is sufficient to mimic an arbitrary tissue. Using a sterile disposable fiber optic probe the diffuse reflectance spectrum of a tissue (either in vivo or ex vivo) is measured, which forms the target spectrum. With the same type of fiber probe, a wide variety of basic components (ingredients) has been previously measured and all together forms a spectral database. A "recipe" for the optimal mixture of ingredients can then be derived using an algorithm that fits the absorption and scattering behavior of the target spectrum using the spectra of the basic components in the database. The spectral mimicking accuracy refines by adding more ingredients to the database. The validity of the principle is demonstrated by mimicking an arbitrary mixture of components. The method can be applied with different kinds of materials, e.g. gelatins, waxes and silicones, thus providing the possibility of mimicking the mechanical properties of target tissues as well. The algorithm can be extended from single point contact spectral measurement to contactless multi- and hyper-spectral camera acquisition. It can be applied to produce portable and durable tissue-like phantoms that provides consistent results over time for calibration, demonstration, comparison of instruments or other such tasks. They are also more readily available than living tissue or a cadaver and are not so limited by ease of handling and legislation; hence they are highly useful when developing new devices.

The Role of Collateral Paths in Long-Range Diffusion of 3He in Lungs

PubMed Central

Conradi, Mark S.; Yablonskiy, Dmitriy A.; Woods, Jason C.; Gierada, David S.; Bartel, Seth-Emil T.; Haywood, Susan E.; Menard, Christopher

2008-01-01

Rationale and Objectives The hyperpolarized 3He long-range diffusion coefficient (LRDC) in lungs is sensitive to changes in lung structure due to emphysema, reflecting the increase in collateral paths resulting from tissue destruction. However, no clear understanding of LRDC in healthy lungs has emerged. Here we compare LRDC measured in healthy lungs with computer simulations of diffusion along the airway tree with no collateral connections. Materials and Methods Computer simulations of diffusion of spatially modulated spin magnetization were performed in computer generated, symmetric-branching models of lungs and compared with existing LRDC measurements in canine and human lungs. Results The simulations predict LRDC values of order 0.001 cm2/s, approximately 20 times smaller than the measured LRDC. We consider and rule out possible mechanisms for LRDC not included in the simulations: incomplete breath hold, cardiac motion, and passage of dissolved 3He through airway walls. However, a very low density of small (micron) holes in the airways is shown to account for the observed LRDC. Conclusion It is proposed that LRDC in healthy lungs is determined by small collateral pathways. PMID:18486004

Development of time-resolved reflectance diffuse optical tomography for breast cancer monitoring

NASA Astrophysics Data System (ADS)

Yoshimoto, Kenji; Ohmae, Etsuko; Yamashita, Daisuke; Suzuki, Hiroaki; Homma, Shu; Mimura, Tetsuya; Wada, Hiroko; Suzuki, Toshihiko; Yoshizawa, Nobuko; Nasu, Hatsuko; Ogura, Hiroyuki; Sakahara, Harumi; Yamashita, Yutaka; Ueda, Yukio

2017-02-01

We developed a time-resolved reflectance diffuse optical tomography (RDOT) system to measure tumor responses to chemotherapy in breast cancer patients at the bedside. This system irradiates the breast with a three-wavelength pulsed laser (760, 800, and 830 nm) through a source fiber specified by an optical switch. The light collected by detector fibers is guided to a detector unit consisting of variable attenuators and photomultiplier tubes. Thirteen irradiation and 12 detection points were set to a measurement area of 50 × 50 mm for a hand-held probe. The data acquisition time required to obtain the temporal profiles within the measurement area is about 2 minutes. The RDOT system generates topographic and tomographic images of tissue properties such as hemoglobin concentration and tissue oxygen saturation using two imaging methods. Topographic images are obtained from the optical properties determined for each source-detector pair using a curve-fitting method based on the photon diffusion theory, while tomographic images are reconstructed using an iterative image reconstruction method. In an experiment using a tissue-like solid phantom, a tumor-like cylindrical target (15 mm diameter, 15 mm high) embedded in a breast tissue-like background medium was successfully reconstructed. Preliminary clinical measurements indicated that the tumor in a breast cancer patient was detected as a region of high hemoglobin concentration. In addition, the total hemoglobin concentration decreased during chemotherapy. These results demonstrate the potential of RDOT for evaluating the effectiveness of chemotherapy in patients with breast cancer.

Monitoring of mirror degradation of fluorescence detectors at the Pierre Auger Observatory due to dust sedimentation

NASA Astrophysics Data System (ADS)

Nozka, L.; Hiklova, H.; Horvath, P.; Hrabovsky, M.; Mandat, D.; Palatka, M.; Pech, M.; Ridky, J.; Schovanek, P.

2018-05-01

We present results of the monitoring method we have used to characterize the optical performance deterioration due to the dust of our mirror segments produced for fluorescence detectors used in astrophysics experiments. The method is based on the measurement of scatter profiles of reflected light. The scatter profiles and the reflectivity of the mirror segments sufficiently describe the performance of the mirrors from the perspective of reconstruction algorithms. The method is demonstrated on our mirror segments installed in frame of the Pierre Auger Observatory project. Although installed in air-conditioned buildings, both the dust sedimentation and the natural aging of the reflective layer deteriorate the optical throughput of the segments. In the paper, we summarized data from ten years of operation of the fluorescence detectors. During this time, we periodically measured in-situ scatter characteristics represented by the specular reflectivity and the reflectivity of the diffusion part at the wavelength of 670 nm of the segment surface (measured by means of the optical scatter technique as well). These measurements were extended with full Bidirectional Reflectance Distribution Functions (BRDF) profiles of selected segments made in the laboratory. Cleaning procedures are also discussed in the paper.

Optical Flow-Field Techniques Used for Measurements in High-Speed Centrifugal Compressors

NASA Technical Reports Server (NTRS)

Skoch, Gary J.

1999-01-01

The overall performance of a centrifugal compressor depends on the performance of the impeller and diffuser as well as on the interactions occurring between these components. Accurate measurements of the flow fields in each component are needed to develop computational models that can be used in compressor design codes. These measurements must be made simultaneously over an area that covers both components so that researchers can understand the interactions occurring between the two components. Optical measurement techniques are being used at the NASA Lewis Research Center to measure the velocity fields present in both the impeller and diffuser of a 4:1 pressure ratio centrifugal compressor operating at several conditions ranging from design flow to surge. Laser Doppler Velocimetry (LDV) was used to measure the intrablade flows present in the impeller, and the results were compared with analyses obtained from two three-dimensional viscous codes. The development of a region of low throughflow velocity fluid within this high-speed impeller was examined and compared with a similar region first observed in a large low-speed centrifugal impeller at Lewis. Particle Image Velocimetry (PIV) is a relatively new technique that has been applied to measuring the diffuser flow fields. PIV can collect data rapidly in the diffuser while avoiding the light-reflection problems that are often encountered when LDV is used. The Particle Image Velocimeter employs a sheet of pulsed laser light that is introduced into the diffuser in a quasi-radial direction through an optical probe inserted near the diffuser discharge. The light sheet is positioned such that its centerline is parallel to the hub and shroud surfaces and such that it is parallel to the diffuser vane, thereby avoiding reflections from the solid surfaces. Seed particles small enough to follow the diffuser flow are introduced into the compressor at an upstream location. A high-speed charge-coupled discharge (CCD) camera is synchronized to the laser pulse rate; this allows it to capture images of seed particle position that are separated by a small increment in time. A crosscorrelation of a particle's position in two consecutive images provides an estimate of flow velocity and direction. Multiple image pairs obtained in rapid succession at a particular flow condition provide enough measurements for statistical significance. PIV provides simultaneous velocity measurements over the entire plane that is illuminated by the light sheet instead of at a single point, as is the case when LDV is used. PIV has a further advantage in that the laser light pulse can be triggered by an external source such as a high-response pressure transducer. This feature will allow PIV to synchronize flow imaging to physical phenomena such as rotating stall or stall precursor waves. We hope that this technique can be used to obtain images of the flow field during and just prior to stall.

Skylab program earth resources experiment package: Ground truth data for test sites (SL-2)

NASA Technical Reports Server (NTRS)

1975-01-01

Field measurements were performed at selected ground sites in order to provide comparative calibration measurements of sensors for the Earth Resources Experiment Package. Specifically, the solar radiation (400 to 1300 namometers) and thermal radiation (8-14 micrometers) were measured. Sites employed for the thermal measurements consisted of warm and cold water lakes. The thermal brightness temperature of the lake water, the temperature and humidity profile above the lake, and near surface meteorology (wind speed, pressure, etc.) were measured near the time of overpass. Sites employed for the solar radiation measurements were two desert type sites. Ground measurements consisted of: (1) direct solar radiation - optical depth; (2) diffuse solar radiation; (3) total solar radiation, (4) target directional (normal) reflectance; (5) target hemispherical reflectance; and (6) near surface meteorology.

Approaching conversion limit with all-dielectric solar cell reflectors.

PubMed

Fu, Sze Ming; Lai, Yi-Chun; Tseng, Chi Wei; Yan, Sheng Lun; Zhong, Yan Kai; Shen, Chang-Hong; Shieh, Jia-Min; Li, Yu-Ren; Cheng, Huang-Chung; Chi, Gou-chung; Yu, Peichen; Lin, Albert

2015-02-09

Metallic back reflectors has been used for thin-film and wafer-based solar cells for very long time. Nonetheless, the metallic mirrors might not be the best choices for photovoltaics. In this work, we show that solar cells with all-dielectric reflectors can surpass the best-configured metal-backed devices. Theoretical and experimental results all show that superior large-angle light scattering capability can be achieved by the diffuse medium reflectors, and the solar cell J-V enhancement is higher for solar cells using all-dielectric reflectors. Specifically, the measured diffused scattering efficiency (D.S.E.) of a diffuse medium reflector is >0.8 for the light trapping spectral range (600nm-1000nm), and the measured reflectance of a diffuse medium can be as high as silver if the geometry of embedded titanium oxide(TiO(2)) nanoparticles is optimized. Moreover, the diffuse medium reflectors have the additional advantage of room-temperature processing, low cost, and very high throughput. We believe that using all-dielectric solar cell reflectors is a way to approach the thermodynamic conversion limit by completely excluding metallic dissipation.

Laboratory measurements of physical, chemical, and optical characteristics of Lake Chicot sediment waters

NASA Technical Reports Server (NTRS)

Witte, W. G.; Whitlock, C. H.; Usry, J. W.; Morris, W. D.; Gurganus, E. A.

1981-01-01

Reflectance, chromaticity, diffuse attenuation, beam attenuation, and several other physical and chemical properties were measured for various water mixtures of lake bottom sediment. Mixture concentrations range from 5 ppm to 700 ppm by weight of total suspended solids in filtered deionized tap water. Upwelled reflectance is a nonlinear function of remote sensing wave lengths. Near-infrared wavelengths are useful for monitoring highly turbid waters with sediment concentrations above 100 ppm. It is found that both visible and near infrared wavelengths, beam attenuation correlates well with total suspended solids ranging over two orders of magnitude.

Infrared Spectroscopy of Explosives Residues: Measurement Techniques and Spectral Analysis

DOE Office of Scientific and Technical Information (OSTI.GOV)

Phillips, Mark C.; Bernacki, Bruce E.

2015-03-11

Infrared laser spectroscopy of explosives is a promising technique for standoff and non-contact detection applications. However, the interpretation of spectra obtained in typical standoff measurement configurations presents numerous challenges. Understanding the variability in observed spectra from explosives residues and particles is crucial for design and implementation of detection algorithms with high detection confidence and low false alarm probability. We discuss a series of infrared spectroscopic techniques applied toward measuring and interpreting the reflectance spectra obtained from explosives particles and residues. These techniques utilize the high spectral radiance, broad tuning range, rapid wavelength tuning, high scan reproducibility, and low noise ofmore » an external cavity quantum cascade laser (ECQCL) system developed at Pacific Northwest National Laboratory. The ECQCL source permits measurements in configurations which would be either impractical or overly time-consuming with broadband, incoherent infrared sources, and enables a combination of rapid measurement speed and high detection sensitivity. The spectroscopic methods employed include standoff hyperspectral reflectance imaging, quantitative measurements of diffuse reflectance spectra, reflection-absorption infrared spectroscopy, microscopic imaging and spectroscopy, and nano-scale imaging and spectroscopy. Measurements of explosives particles and residues reveal important factors affecting observed reflectance spectra, including measurement geometry, substrate on which the explosives are deposited, and morphological effects such as particle shape, size, orientation, and crystal structure.« less

Fractional order analysis of Sephadex gel structures: NMR measurements reflecting anomalous diffusion

NASA Astrophysics Data System (ADS)

Magin, Richard L.; Akpa, Belinda S.; Neuberger, Thomas; Webb, Andrew G.

2011-12-01

We report the appearance of anomalous water diffusion in hydrophilic Sephadex gels observed using pulse field gradient (PFG) nuclear magnetic resonance (NMR). The NMR diffusion data was collected using a Varian 14.1 Tesla imaging system with a home-built RF saddle coil. A fractional order analysis of the data was used to characterize heterogeneity in the gels for the dynamics of water diffusion in this restricted environment. Several recent studies of anomalous diffusion have used the stretched exponential function to model the decay of the NMR signal, i.e., exp[-( bD) α], where D is the apparent diffusion constant, b is determined the experimental conditions (gradient pulse separation, durations and strength), and α is a measure of structural complexity. In this work, we consider a different case where the spatial Laplacian in the Bloch-Torrey equation is generalized to a fractional order model of diffusivity via a complexity parameter, β, a space constant, μ, and a diffusion coefficient, D. This treatment reverts to the classical result for the integer order case. The fractional order decay model was fit to the diffusion-weighted signal attenuation for a range of b-values (0 < b < 4000 s mm -2). Throughout this range of b values, the parameters β, μ and D, were found to correlate with the porosity and tortuosity of the gel structure.

Quantification of tissue oxygenation levels using diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

B. S., Suresh Anand; N., Sujatha

2011-08-01

Tumor growth is characterized by increased metabolic activity. The light absorption profile of hemoglobin in dysplastic tissue is different from a normal tissue. Neovascularization is a hallmark of many diseases and can serve as a predictive biomarker for the detection of cancers. Spectroscopic techniques can provide information about the metabolic and morphological changes related to the progression of neoplasia. Diffuse reflectance spectroscopy (DRS) measures the absorption and scattering properties of a biological tissue and this method can provide clinically useful information for the early diagnosis of epithelial precancers. We used tissue simulating phantoms with absorbing and scattering molecules for the determination of total hemoglobin concentration, hemoglobin oxygen saturation and intensity difference between the deoxy and oxy hemoglobin bands. The results show promising approach for the differentiating normal and malignant states of a tissue.

Pectin functionalised by fatty acids: Diffuse reflectance infrared Fourier transform (DRIFT) spectroscopic characterisation

NASA Astrophysics Data System (ADS)

Kamnev, Alexander A.; Calce, Enrica; Tarantilis, Petros A.; Tugarova, Anna V.; De Luca, Stefania

2015-01-01

Chemically modified pectin derivatives obtained by partial esterification of its hydroxyl moieties with fatty acids (FA; oleic, linoleic and palmitic acids), as well as the initial apple peel pectin were comparatively characterised using diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy. Characteristic changes observed in DRIFT spectra in going from pectin to its FA esters are related to the corresponding chemical modifications. Comparing the DRIFT spectra with some reported data on FTIR spectra of the same materials measured in KBr or NaCl matrices has revealed noticeable shifts of several polar functional groups both in pectin and in its FA-esterified products induced by the halide salts. The results obtained have implications for careful structural analyses of biopolymers with hydrophilic functional groups by means of different FTIR spectroscopic methodologies.

Hazard calculations of diffuse reflected laser radiation for the SELENE program

NASA Technical Reports Server (NTRS)

Miner, Gilda A.; Babb, Phillip D.

1993-01-01

The hazards from diffuse laser light reflections off water clouds, ice clouds, and fog and from possible specular reflections off ice clouds were assessed with the American National Standards (ANSI Z136.1-1986) for the free-electron-laser parameters under consideration for the Segmented Efficient Laser Emission for Non-Nuclear Electricity (SELENE) Program. Diffuse laser reflection hazards exist for water cloud surfaces less than 722 m in altitude and ice cloud surfaces less than 850 m in altitude. Specular reflections from ice crystals in cirrus clouds are not probable; however, any specular reflection is a hazard to ground observers. The hazard to the laser operators and any ground observers during heavy fog conditions is of such significant magnitude that the laser should not be operated in fog.

Flat-plate techniques for measuring reflectance of macro-algae (Ulva curvata)

USGS Publications Warehouse

Ramsey, Elijah W.; Rangoonwala, Amina; Thomsen, Mads Solgaard; Schwarzschild, Arthur

2012-01-01

We tested the consistency and accuracy of flat-plate spectral measurements (400–1000 nm) of the marine macrophyte Ulva curvata. With sequential addition of Ulva thallus layers, the reflectance progressively increased from 6% to 9% with six thalli in the visible (VIS) and from 5% to 19% with ten thalli in the near infrared (NIR). This progressive increase was simulated by a mathematical calculation based on an Ulva thallus diffuse reflectance weighted by a transmittance power series. Experimental and simulated reflectance differences that were particularly high in the NIR most likely resulted from residual water and layering structure unevenness in the experimental progression. High spectral overlap existed between fouled and non-fouled Ulva mats and the coexistent lagoon mud in the VIS, whereas in the NIR, spectral contrast was retained but substantially dampened by fouling.

Optical inverse-square displacement sensor

DOEpatents

Howe, R.D.; Kychakoff, G.

1989-09-12

This invention comprises an optical displacement sensor that uses the inverse-square attenuation of light reflected from a diffused surface to calculate the distance from the sensor to the reflecting surface. Light emerging from an optical fiber or the like is directed onto the surface whose distance is to be measured. The intensity I of reflected light is angle dependent, but within a sufficiently small solid angle it falls off as the inverse square of the distance from the surface. At least a pair of optical detectors are mounted to detect the reflected light within the small solid angle, their ends being at different distances R and R + [Delta]R from the surface. The distance R can then be found in terms of the ratio of the intensity measurements and the separation length as given in an equation. 10 figs.

Improved algorithm for estimating optical properties of food and biological materials using spatially-resolved diffuse reflectance

USDA-ARS?s Scientific Manuscript database

In this research, the inverse algorithm for estimating optical properties of food and biological materials from spatially-resolved diffuse reflectance was optimized in terms of data smoothing, normalization and spatial region of reflectance profile for curve fitting. Monte Carlo simulation was used ...

Evolution of diffusion and dissemination theory.

PubMed

Dearing, James W

2008-01-01

The article provides a review and considers how the diffusion of innovations Research paradigm has changed, and offers suggestions for the further development of this theory of social change. Main emphases of diffusion Research studies are compared over time, with special attention to applications of diffusion theory-based concepts as types of dissemination science. A considerable degree of paradigmatic evolution is observed. The classical diffusion model focused on adopter innovativeness, individuals as the locus of decision, communication channels, and adoption as the primary outcome measures in post hoc observational study designs. The diffusion systems in question were centralized, with fidelity of implementation often assumed. Current dissemination Research and practice is better characterized by tests of interventions that operationalize one or more diffusion theory-based concepts and concepts from other change approaches, involve complex organizations as the units of adoption, and focus on implementation issues. Foment characterizes dissemination and implementation Research, Reflecting both its interdisciplinary Roots and the imperative of spreading evidence-based innovations as a basis for a new paradigm of translational studies of dissemination science.

Noncontact discrimination of animal and human blood with vacuum blood vessel and factors affect the discrimination

NASA Astrophysics Data System (ADS)

Zhang, Linna; Zhang, Shengzhao; Sun, Meixiu; Li, Hongxiao; Li, Yingxin; Fu, Zhigang; Guan, Yang; Li, Gang; Lin, Ling

2017-03-01

Discrimination of human and nonhuman blood is crucial for import-export ports and inspection and quarantine departments. Current methods are usually destructive, complicated and time-consuming. We had previously demonstrated that visible diffuse reflectance spectroscopy combining PLS-DA method can successfully realize human blood discrimination. In that research, the spectra were measured with the fiber probe under the surface of blood samples. However, open sampling may pollute the blood samples. Virulence factors in blood samples can also endanger inspectors. In this paper, we explored the classification effect with the blood samples measured in the original containers-vacuum blood vessel. Furthermore, we studied the impact of different conditions of blood samples, such as coagulation and hemolysis, on the prediction ability of the discrimination model. The calibration model built with blood samples in different conditions displayed a satisfactory prediction result. This research demonstrated that visible and near-infrared diffuse reflectance spectroscopy method was potential for noncontact discrimination of human blood.

Mapping the conduction band edge density of states of γ-In2Se3 by diffuse reflectance spectra

NASA Astrophysics Data System (ADS)

Kumar, Pradeep; Vedeshwar, Agnikumar G.

2018-03-01

It is demonstrated that the measured diffuse reflectance spectra of γ-In2Se3 can be used to map the conduction band edge density of states through Kubelka-Munk analysis. The Kubelka-Munk function derived from the measured spectra almost mimics the calculated density of states in the vicinity of conduction band edge. The calculation of density of states was carried out using first-principles approach yielding the structural, electronic, and optical properties. The calculations were carried out implementing various functionals and only modified Tran and Blaha (TB-MBJ) results tally closest with the experimental result of band gap. The electronic and optical properties were calculated using FP-LAPW + lo approach based on the Density Functional Theory formalism implementing only TB-mBJ functional. The electron and hole effective masses have been calculated as me * = 0.25 m 0 and mh * = 1.11 m 0 , respectively. The optical properties clearly indicate the anisotropic nature of γ-In2Se3.

Properties of seismic absorption induced reflections

NASA Astrophysics Data System (ADS)

Zhao, Haixia; Gao, Jinghuai; Peng, Jigen

2018-05-01

Seismic reflections at an interface are often regarded as the variation of the acoustic impedance (product of seismic velocity and density) in a medium. In fact, they can also be generated due to the difference in absorption of the seismic energy. In this paper, we investigate the properties of such reflections. Based on the diffusive-viscous wave equation and elastic diffusive-viscous wave equation, we investigate the dependency of the reflection coefficients on frequency, and their variations with incident angles. Numerical results at a boundary due to absorption contrasts are compared with those resulted from acoustic impedance variation. It is found that, the reflection coefficients resulted from absorption depend significantly on the frequency especially at lower frequencies, but vary very slowly at small incident angles. At the higher frequencies, the reflection coefficients of diffusive-viscous wave and elastic diffusive-viscous wave are close to those of acoustic and elastic cases, respectively. On the other hand, the reflections caused by acoustic impedance variation are independent of frequency but vary distinctly with incident angles before the critical angle. We also investigate the difference between the seismograms generated in the two different media. The numerical results show that the amplitudes of these reflected waves are attenuated and their phases are shifted. However, the reflections obtained by acoustic impedance contrast, show no significant amplitude attenuation and phase shift.

k-Space Image Correlation Spectroscopy: A Method for Accurate Transport Measurements Independent of Fluorophore Photophysics

PubMed Central

Kolin, David L.; Ronis, David; Wiseman, Paul W.

2006-01-01

We present the theory and application of reciprocal space image correlation spectroscopy (kICS). This technique measures the number density, diffusion coefficient, and velocity of fluorescently labeled macromolecules in a cell membrane imaged on a confocal, two-photon, or total internal reflection fluorescence microscope. In contrast to r-space correlation techniques, we show kICS can recover accurate dynamics even in the presence of complex fluorophore photobleaching and/or “blinking”. Furthermore, these quantities can be calculated without nonlinear curve fitting, or any knowledge of the beam radius of the exciting laser. The number densities calculated by kICS are less sensitive to spatial inhomogeneity of the fluorophore distribution than densities measured using image correlation spectroscopy. We use simulations as a proof-of-principle to show that number densities and transport coefficients can be extracted using this technique. We present calibration measurements with fluorescent microspheres imaged on a confocal microscope, which recover Stokes-Einstein diffusion coefficients, and flow velocities that agree with single particle tracking measurements. We also show the application of kICS to measurements of the transport dynamics of α5-integrin/enhanced green fluorescent protein constructs in a transfected CHO cell imaged on a total internal reflection fluorescence microscope using charge-coupled device area detection. PMID:16861272

Diffuse Reflectance Spectroscopy for Surface Measurement of Liver Pathology.

PubMed

Nilsson, Jan H; Reistad, Nina; Brange, Hannes; Öberg, Carl-Fredrik; Sturesson, Christian

2017-01-01

Liver parenchymal injuries such as steatosis, steatohepatitis, fibrosis, and sinusoidal obstruction syndrome can lead to increased morbidity and liver failure after liver resection. Diffuse reflectance spectroscopy (DRS) is an optical measuring method that is fast, convenient, and established. DRS has previously been used on the liver with an invasive technique consisting of a needle that is inserted into the parenchyma. We developed a DRS system with a hand-held probe that is applied to the liver surface. In this study, we investigated the impact of the liver capsule on DRS measurements and whether liver surface measurements are representative of the whole liver. We also wanted to confirm that we could discriminate between tumor and liver parenchyma by DRS. The instrumentation setup consisted of a light source, a fiber-optic contact probe, and two spectrometers connected to a computer. Patients scheduled for liver resection due to hepatic malignancy were included, and DRS measurements were performed on the excised liver part with and without the liver capsule and alongside a newly cut surface. To estimate the scattering parameters and tissue chromophore volume fractions, including blood, bile, and fat, the measured diffuse reflectance spectra were applied to an analytical model. In total, 960 DRS spectra from the excised liver tissue of 18 patients were analyzed. All factors analyzed regarding tumor versus liver tissue were significantly different. When measuring through the capsule, the blood volume fraction was found to be 8.4 ± 3.5%, the lipid volume fraction was 9.9 ± 4.7%, and the bile volume fraction was 8.2 ± 4.6%. No differences could be found between surface measurements and cross-sectional measurements. In measurements with/without the liver capsule, the differences in volume fraction were 1.63% (0.75-2.77), -0.54% (-2.97 to 0.32), and -0.15% (-1.06 to 1.24) for blood, lipid, and bile, respectively. This study shows that it is possible to manage DRS measurements through the liver capsule and that surface DRS measurements are representative of the whole liver. The results are consistent with data published earlier on the combination of liver chromophores. The results encourage us to proceed with in vivo measurements for further quantification of the liver's composition and assessment of parenchymal damage such as steatosis and fibrosis grade. © 2016 S. Karger AG, Basel.

Multispectral detection of cutaneous lesions using spectroscopy and microscopy approaches

NASA Astrophysics Data System (ADS)

Borisova, E.; Genova-Hristova, Ts.; Troyanova, P.; Pavlova, E.; Terziev, I.; Semyachkina-Glushkovskaya, O.; Lomova, M.; Genina, E.; Stanciu, G.; Tranca, D.; Avramov, L.

2018-02-01

Autofluorescence, diffuse-reflectance and transmission spectral, and microscopic measurements were made on different cutaneous neoplastic lesions, namely basal cell carcinoma, squamous cell carcinoma, malignant melanoma, and dysplastic and benign lesions related. Spectroscopic measurements were made on ex vivo tissue samples, and confocal microscopy investigations were made on thin tissue slices. Fluorescence spectra obtained reveal statistically significant differences between the different benign, dysplastic and malignant lesions by the level of emission intensity, as well by spectral shape, which are fingerprints applicable for differentiation algorithms. In reflectance mode the most significant differences are related to the influence of skin pigments - melanin and hemoglobin. Transmission spectroscopy mode gave complementary optical properties information about the tissue samples investigated to that one of reflectance and absorption spectroscopy. Using autofluorescence detection of skin lesions we obtain very good diagnostic performance for distinguishing of nonmelanoma lesions. Using diffuse reflectance and transmission spectroscopy we obtain significant tool for pigmented pathologies differentiation, but it is a tool with moderate sensitivity for non-melanoma lesions detection. One could rapidly increase the diagnostic accuracy of the received combined "optical biopsy" method when several spectral detection techniques are applied in common algorithm for lesions' differentiation. Specific spectral features observed in each type of lesion investigated on micro and macro level would be presented and discussed. Correlation between the spectral data received and the microscopic features observed would be discussed in the report.

A hybrid HDRF model of GOMS and SAIL: GOSAIL

NASA Astrophysics Data System (ADS)

Dou, B.; Wu, S.; Wen, J.

2016-12-01

Understanding the surface reflectance anisotropy is the key facet in interpreting the features of land surface from remotely sensed information, which describes the property of land surface to reflect the solar radiation directionally. Most reflectance anisotropy models assumed the nature surface was illuminated only by the direct solar radiation, while the diffuse skylight becomes dominant especially for the over cast sky conditions and high rugged terrain. Correcting the effect of diffuse skylight on the reflectance anisotropy to obtain the intrinsic directional reflectance of land surface is highly desirable for remote sensing applications. This paper developed a hybrid HDRF model of GOMS and SAIL called GOSAIL model for discrete canopies. The accurate area proportions of four scene components are calculated by the GOMS model and the spectral signatures of scene components are provided by the SAIL model. Both the single scattering contribution and the multiple scattering contributions within and between the canopy and background under the clear and diffuse illumination conditions are considered in the GOSAIL model. The HDRF simulated by the 3-D Discrete Anisotropic Radiative Transfer (DART) model and the HDRF measurements over the 100m×100m mature pine stand at the Järvselja, Estonia are used for validating and evaluating the performance of proposed GOSAIL model. The comparison results indicate the GOSAIL model can accurately reproducing the angular feature of discrete canopy for both the clear and overcast atmospheric conditions. The GOSAIL model is promising for the land surface biophysical parameters retrieval (e.g. albedo, leaf area index) over the heterogeneous terrain.

New mounting improves solar-cell efficiency

NASA Technical Reports Server (NTRS)

Shepard, N. F., Jr.

1980-01-01

Method boosts output by about 20 percent by trapping and redirecting solar radiation without increasing module depth. Mounted solar-cell array is covered with internally reflecting plate. Plate is attached to each cell by transparent adhesive, and space between cells is covered with layer of diffusely reflecting material. Solar energy falling on space between cells is diffused and reflected internally by plate until it is reflected onto solar cell.

Anisotropic reflectance from turbid media. I. Theory.

PubMed

Neuman, Magnus; Edström, Per

2010-05-01

It is shown that the intensity of light reflected from plane-parallel turbid media is anisotropic in all situations encountered in practice. The anisotropy, in the form of higher intensity at large polar angles, increases when the amount of near-surface bulk scattering is increased, which dominates in optically thin and highly absorbing media. The only situation with isotropic intensity is when a non-absorbing infinitely thick medium is illuminated diffusely. This is the only case where the Kubelka-Munk model gives exact results and there exists an exact translation between Kubelka-Munk and general radiative transfer. This also means that a bulk scattering perfect diffusor does not exist. Angle-resolved models are thus crucial for a correct understanding of light scattering in turbid media. The results are derived using simulations and analytical calculations. It is also shown that there exists an optimal angle for directional detection that minimizes the error introduced when using the Kubelka-Munk model to interpret reflectance measurements with diffuse illumination.

Leaf water stress detection utilizing thematic mapper bands 3, 4 and 5 in soybean plants

NASA Technical Reports Server (NTRS)

Holben, B. N.; Schutt, J. B.; Mcmurtrey, J., III

1983-01-01

The total and diffuse radiance responses of Thematic Mapper bands 3 (0.63-0.69 microns), 4 (0.76-0.90 microns), and 5 (1.55-1.75 microns) to water stress in a soybean canopy are compared. Polarization measurements were used to separate the total from the diffuse reflectance; the reflectances were compared statistically at a variety of look angles at 15 min intervals from about 09.00 until 14.00 hrs EST. The results suggest that remotely sensed data collected in the photographic infrared region (TM4) are sensitive to leaf water stress in a 100 percent canopy cover of soybeans, and that TM3 is less sensitive than TM4 for detection of reversible foliar water stress. The mean values of TM5 reflectance data show similar trends to TM4. The primary implication of this study is that remote sensing of water stress in green plant canopies is possible in TM4 from ground-based observations primarily through the indirect link of leaf geometry.

Testing the Li-Strahler four-component canopy reflectance model in the HAPEX-Sahel shrub savanna sites using ground reflectance data

NASA Technical Reports Server (NTRS)

Franklin, J.; Duncan, J.

1992-01-01

The rate at which a light field decays in water is characterized by the diffuse attenuation coefficient k. The Li-Strahler discrete-object canopy reflectance model was tested in two sites, a shrub grass savanna and a degraded shrub savanna on bare soil, in the proposed HAPEX (Hydrologic Atmospheric Pilot Experiment) II/Sahel study area in Niger, West Africa. Average site reflectance was predicted for each site from the reflectances and cover proportions of four components: shrub canopy, background (soil or grass and soil), shaded canopy, and shaded background. Component reflectances were sampled in the SPOT wavebands using a hand-held radiometer. Predicted reflectance was compared to average site reflectance measured using the same radiometer mounted on a backpack with measurements recorded every 5 m along two 1-km transects, also in the SPOT (Systeme Probatoire d'Observation de la Terre) bands. Measurements and predictions were made for each of the three days during the summer growing season, approximately two weeks apart. Red, near infrared reflectance, and the NDVI (normalized difference vegetation index) were all predicted with a high degree of accuracy for the shrub/grass site and with reasonable accuracy for the degraded shrub site.

Optical luminescence studies of diffusion times at the potassium ethyl xanthate adsorption layer on the surface of sphalerite minerals

NASA Astrophysics Data System (ADS)

Todoran, R.; Todoran, D.; Anitas, E. M.; Szakács, Zs

2016-08-01

We propose reflectance measurements as a method for the evaluation of the kinetics of adsorption processes, to compute the diffusion times of the adsorption products at the thin layers formed at the sphalerite natural mineral-potassium ethyl xanthate solution interface. The method is based on the intensity measurement of the reflected monochromatic radiation obtained from the mineral-xanthate thin layer as a function of time. These determinations were made at the thin layer formed between the sphalerite or activated sphalerite natural minerals with potassium ethyl xanthate, for different solutions concentrations and pH values at constant temperature. Diffusion times of desorbed molecular species into the liquid bring important information about the global kinetics of the ions in this phase during adsorption processes at interfaces. Analysing the time dependence of this parameter one concluded on the diffusion properties of the xanthate molecule in the solution depending on its concentration and pH, knowing that at the initial time these molecules had a uniform spread. This method enabled us to determine that, in time interval of approximately 35 minutes to achieve dynamic equilibrium in the formation of the interface layer, one had three different kinetic behaviours of our systems. In the first 5-8 min one had highly adsorbent character, the state of equilibrium is followed by low adsorbent properties. Gaining information on the adsorption kinetics in the case of xanthate on mineral surface leads to the optimization of the industrial froth flotation process.

Brain tissue oxygen tension is more indicative of oxygen diffusion than oxygen delivery and metabolism in patients with traumatic brain injury.

PubMed

Rosenthal, Guy; Hemphill, J Claude; Sorani, Marco; Martin, Christine; Morabito, Diane; Obrist, Walter D; Manley, Geoffrey T

2008-06-01

Despite the growing clinical use of brain tissue oxygen monitoring, the specific determinants of low brain tissue oxygen tension (P(bt)O2) following severe traumatic brain injury (TBI) remain poorly defined. The objective of this study was to evaluate whether P(bt)O2 more closely reflects variables related to cerebral oxygen diffusion or reflects cerebral oxygen delivery and metabolism. Prospective observational study. Level I trauma center. Fourteen TBI patients with advanced neuromonitoring underwent an oxygen challenge (increase in FiO2 to 1.0) to assess tissue oxygen reactivity, pressure challenge (increase in mean arterial pressure) to assess autoregulation, and CO2 challenge (hyperventilation) to assess cerebral vasoreactivity. None. P(bt)O2 was measured directly with a parenchymal probe in the least-injured hemisphere. Local cerebral blood flow (CBF) was measured with a parenchymal thermal diffusion probe. Cerebral venous blood gases were drawn from a jugular bulb venous catheter. We performed 119 measurements of PaO2, arterial oxygen content (CaO2), jugular bulb venous oxygen tension (PVO2), venous oxygen content (CVO2), arteriovenous oxygen content difference (AVDO2), and local cerebral metabolic rate of oxygen (locCMRO2). In multivariable analysis adjusting for various variables of cerebral oxygen delivery and metabolism, the only statistically significant relationship was that between P(bt)O2 and the product of CBF and cerebral arteriovenous oxygen tension difference (AVTO2), suggesting a strong association between brain tissue oxygen tension and diffusion of dissolved plasma oxygen across the blood-brain barrier. Measurements of P(bt)O2 represent the product of CBF and the cerebral AVTO2 rather than a direct measurement of total oxygen delivery or cerebral oxygen metabolism. This improved understanding of the cerebral physiology of P(bt)O2 should enhance the clinical utility of brain tissue oxygen monitoring in patients with TBI.

Multiplexed Colorimetric Solid-Phase Extraction

NASA Technical Reports Server (NTRS)

Gazda, Daniel B.; Fritz, James S.; Porter, Marc D.

2009-01-01

Multiplexed colorimetric solid-phase extraction (MC-SPE) is an extension of colorimetric solid-phase extraction (C-SPE) an analytical platform that combines colorimetric reagents, solid phase extraction, and diffuse reflectance spectroscopy to quantify trace analytes in water. In CSPE, analytes are extracted and complexed on the surface of an extraction membrane impregnated with a colorimetric reagent. The analytes are then quantified directly on the membrane surface using a handheld diffuse reflectance spectrophotometer. Importantly, the use of solid-phase extraction membranes as the matrix for impregnation of the colorimetric reagents creates a concentration factor that enables the detection of low concentrations of analytes in small sample volumes. In extending C-SPE to a multiplexed format, a filter holder that incorporates discrete analysis channels and a jig that facilitates the concurrent operation of multiple sample syringes have been designed, enabling the simultaneous determination of multiple analytes. Separate, single analyte membranes, placed in a readout cartridge create unique, analyte-specific addresses at the exit of each channel. Following sample exposure, the diffuse reflectance spectrum of each address is collected serially and the Kubelka-Munk function is used to quantify each water quality parameter via calibration curves. In a demonstration, MC-SPE was used to measure the pH of a sample and quantitate Ag(I) and Ni(II).

Simultaneous estimation of transcutaneous bilirubin, hemoglobin, and melanin based on diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Nishidate, Izumi; Abdul, Wares MD.; Ohtsu, Mizuki; Nakano, Kazuya; Haneishi, Hideaki

2018-02-01

We propose a method to estimate transcutaneous bilirubin, hemoglobin, and melanin based on the diffuse reflectance spectroscopy. In the proposed method, the Monte Carlo simulation-based multiple regression analysis for an absorbance spectrum in the visible wavelength region (460-590 nm) is used to specify the concentrations of bilirubin (Cbil), oxygenated hemoglobin (Coh), deoxygenated hemoglobin (Cdh), and melanin (Cm). Using the absorbance spectrum calculated from the measured diffuse reflectance spectrum as a response variable and the extinction coefficients of bilirubin, oxygenated hemoglobin, deoxygenated hemoglobin, and melanin, as predictor variables, multiple regression analysis provides regression coefficients. Concentrations of bilirubin, oxygenated hemoglobin, deoxygenated hemoglobin, and melanin, are then determined from the regression coefficients using conversion vectors that are numerically deduced in advance by the Monte Carlo simulations for light transport in skin. Total hemoglobin concentration (Cth) and tissue oxygen saturation (StO2) are simply calculated from the oxygenated hemoglobin and deoxygenated hemoglobin. In vivo animal experiments with bile duct ligation in rats demonstrated that the estimated Cbil is increased after ligation of bile duct and reaches to around 20 mg/dl at 72 h after the onset of the ligation, which corresponds to the reference value of Cbil measured by a commercially available transcutaneous bilirubin meter. We also performed in vivo experiments with rats while varying the fraction of inspired oxygen (FiO2). Coh and Cdh decreased and increased, respectively, as FiO2 decreased. Consequently, StO2 was dramatically decreased. The results in this study indicate potential of the method for simultaneous evaluation of multiple chromophores in skin tissue.

Systematic analysis of diffuse rear reflectors for enhanced light trapping in silicon solar cells

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pfeffer, Florian; Eisenlohr, Johannes; Basch, Angelika

Simple diffuse rear reflectors can enhance the light path length of weakly absorbed near infrared light in silicon solar cells and set a benchmark for more complex and expensive light trapping structures like dielectric gratings or plasmonic particles. We analyzed such simple diffuse rear reflectors systematically by optical and electrical measurements. We applied white paint, TiO 2 nanoparticles, white backsheets and a silver mirror to bifacial silicon solar cells and measured the enhancement of the external quantum efficiency for three different solar cell geometries: planar front and rear side, textured front and planar rear side, and textured front and rearmore » side. We showed that an air-gap between the solar cell and the reflector decreases the absorption enhancement significantly, thus white paint and TiO 2 nanoparticles directly applied to the rear cell surface lead to the highest short circuit current density enhancements. Here, the short circuit current density gains for a 200 um thick planar solar cell reached up to 1.8 mA/cm 2, compared to a non-reflecting black rear side and up to 0.8 mA/cm 2 compared to a high-quality silver mirror rear side. For solar cells with textured front side the short circuit current density gains are in the range between 0.5 and 1.0 mA/cm 2 compared to a non-reflecting black rear side and do not significantly depend on the angular characteristic of the rear side reflector but mainly on its absolute reflectance.« less

Systematic analysis of diffuse rear reflectors for enhanced light trapping in silicon solar cells

DOE PAGES

Pfeffer, Florian; Eisenlohr, Johannes; Basch, Angelika; ...

2016-04-08

Simple diffuse rear reflectors can enhance the light path length of weakly absorbed near infrared light in silicon solar cells and set a benchmark for more complex and expensive light trapping structures like dielectric gratings or plasmonic particles. We analyzed such simple diffuse rear reflectors systematically by optical and electrical measurements. We applied white paint, TiO 2 nanoparticles, white backsheets and a silver mirror to bifacial silicon solar cells and measured the enhancement of the external quantum efficiency for three different solar cell geometries: planar front and rear side, textured front and planar rear side, and textured front and rearmore » side. We showed that an air-gap between the solar cell and the reflector decreases the absorption enhancement significantly, thus white paint and TiO 2 nanoparticles directly applied to the rear cell surface lead to the highest short circuit current density enhancements. Here, the short circuit current density gains for a 200 um thick planar solar cell reached up to 1.8 mA/cm 2, compared to a non-reflecting black rear side and up to 0.8 mA/cm 2 compared to a high-quality silver mirror rear side. For solar cells with textured front side the short circuit current density gains are in the range between 0.5 and 1.0 mA/cm 2 compared to a non-reflecting black rear side and do not significantly depend on the angular characteristic of the rear side reflector but mainly on its absolute reflectance.« less

Optical measurements of absorption changes in two-layered diffusive media

NASA Astrophysics Data System (ADS)

Fabbri, Francesco; Sassaroli, Angelo; Henry, Michael E.; Fantini, Sergio

2004-04-01

We have used Monte Carlo simulations for a two-layered diffusive medium to investigate the effect of a superficial layer on the measurement of absorption variations from optical diffuse reflectance data processed by using: (a) a multidistance, frequency-domain method based on diffusion theory for a semi-infinite homogeneous medium; (b) a differential-pathlength-factor method based on a modified Lambert-Beer law for a homogeneous medium and (c) a two-distance, partial-pathlength method based on a modified Lambert-Beer law for a two-layered medium. Methods (a) and (b) lead to a single value for the absorption variation, whereas method (c) yields absorption variations for each layer. In the simulations, the optical coefficients of the medium were representative of those of biological tissue in the near-infrared. The thickness of the first layer was in the range 0.3-1.4 cm, and the source-detector distances were in the range 1-5 cm, which is typical of near-infrared diffuse reflectance measurements in tissue. The simulations have shown that (1) method (a) is mostly sensitive to absorption changes in the underlying layer, provided that the thickness of the superficial layer is ~0.6 cm or less; (2) method (b) is significantly affected by absorption changes in the superficial layer and (3) method (c) yields the absorption changes for both layers with a relatively good accuracy of ~4% for the superficial layer and ~10% for the underlying layer (provided that the absorption changes are less than 20-30% of the baseline value). We have applied all three methods of data analysis to near-infrared data collected on the forehead of a human subject during electroconvulsive therapy. Our results suggest that the multidistance method (a) and the two-distance partial-pathlength method (c) may better decouple the contributions to the optical signals that originate in deeper tissue (brain) from those that originate in more superficial tissue layers.

Reduction of skylight reflection effects in the above-water measurement of diffuse marine reflectance: comment.

PubMed

Krotkov, N A; Vasilkov, A P

2000-03-20

Use of a vertical polarizer has been suggested to reduce the effects of surface reflection in the above-water measurements of marine reflectance. We suggest using a similar technique for airborne or spaceborne sensors when atmospheric scattering adds its own polarization signature to the upwelling radiance. Our own theoretical sensitivity study supports the recommendation of Fougnie et al. [Appl. Opt. 38, 3844 (1999)] (40-50 degrees vertical angle and azimuth angle near 135 degrees, polarizer parallel to the viewing plane) for above-water measurements. However, the optimal viewing directions (and the optimal orientation of the polarizer) change with altitude above the sea surface, solar angle, and atmospheric vertical optical structure. A polarization efficiency function is introduced, which shows the maximal possible polarization discrimination of the background radiation for an arbitrary altitude above the sea surface, viewing direction, and solar angle. Our comment is meant to encourage broader application of airborne and spaceborne polarization sensors in remote sensing of water and sea surface properties.

Far-ultraviolet Bidirectional Photometry of Apollo Soil 10084: New Results from The Southwest Ultraviolet Reflectance Chamber (SwURC).

NASA Astrophysics Data System (ADS)

Raut, U.

2017-12-01

We report new measurements of the far-ultraviolet (115-180 nm) bidirectional reflectance of Apollo soil 10084 in the Southwest Ultraviolet Reflectance Chamber (SwURC). We find the bidirectional reflectance distribution function (BRDF) to be featureless in this wavelength region, though with a small blue slope. The angular distribution of the BRDF at Ly-α and 160 nm shows that this mature mare soil, containing nanophase Fe and enriched in Ti, anisotropically scatters light in the forward direction. The phase angle dependence of the BRDF is fitted with Hapke's photometric model with an additional diffuse-directional term. Future plans include measurements of mare and highland soils of differing maturity index (Is/FeO), water ice frost and lunar soil-ice aggregates. Such measurements will help constrain the abundance and distribution of the water ice on the illuminated lunar surface and dark permanently shadowed regions of the moon, as reported by LRO-LAMP.

The γ parameter of the stretched-exponential model is influenced by internal gradients: validation in phantoms.

PubMed

Palombo, Marco; Gabrielli, Andrea; De Santis, Silvia; Capuani, Silvia

2012-03-01

In this paper, we investigate the image contrast that characterizes anomalous and non-gaussian diffusion images obtained using the stretched exponential model. This model is based on the introduction of the γ stretched parameter, which quantifies deviation from the mono-exponential decay of diffusion signal as a function of the b-value. To date, the biophysical substrate underpinning the contrast observed in γ maps, in other words, the biophysical interpretation of the γ parameter (or the fractional order derivative in space, β parameter) is still not fully understood, although it has already been applied to investigate both animal models and human brain. Due to the ability of γ maps to reflect additional microstructural information which cannot be obtained using diffusion procedures based on gaussian diffusion, some authors propose this parameter as a measure of diffusion heterogeneity or water compartmentalization in biological tissues. Based on our recent work we suggest here that the coupling between internal and diffusion gradients provide pseudo-superdiffusion effects which are quantified by the stretching exponential parameter γ. This means that the image contrast of Mγ maps reflects local magnetic susceptibility differences (Δχ(m)), thus highlighting better than T(2)(∗) contrast the interface between compartments characterized by Δχ(m). Thanks to this characteristic, Mγ imaging may represent an interesting tool to develop contrast-enhanced MRI for molecular imaging. The spectroscopic and imaging experiments (performed in controlled micro-beads dispersion) that are reported here, strongly suggest internal gradients, and as a consequence Δχ(m), to be an important factor in fully understanding the source of contrast in anomalous diffusion methods that are based on a stretched exponential model analysis of diffusion data obtained at varying gradient strengths g. Copyright © 2012 Elsevier Inc. All rights reserved.

Non-Lambertian effects on remote sensing of surface reflectance and vegetation index

NASA Technical Reports Server (NTRS)

Lee, T. Y.; Kaufman, Y. J.

1986-01-01

This paper discusses the effects of non-Lambertian reflection from a homogeneous surface on remote sensing of the surface reflectance and vegetation index from a satellite. Remote measurement of the surface characteristics is perturbed by atmospheric scattering of sun light. This scattering tends to smooth the angular dependence of non-Lambertian surface reflectances, an effect that is not present in the case of Lambertian surfaces. This effect is calculated to test the validity of a Lambertian assumption used in remote sensing. For the three types of vegetations considered in this study, the assumption of Lambertian surface can be used satisfactorily in the derivation of surface reflectance from remotely measured radiance for a view angle outside the backscattering region. Within the backscattering region, however, the use of the assumption can result in a considerable error in the derived surface reflectance. Accuracy also deteriorates with increasing solar zenith angle. The angular distribution of the surface reflectance derived from remote measurements is smoother than that at the surface. The effect of surface non-Lambertianity on remote sensing of vegetation index is very weak. Since the effect is similiar in the visible and near infrared part of the solar spectrum for the vegetations treated in this study, it is canceled in deriving the vegetation index. The effect of the diffuse skylight on surface reflectance measurements at ground level is also discussed.

National Institute of Standards and Technology measurement service of the optical properties of biomedical phantoms: Current status

PubMed Central

Lemaillet, Paul; Cooksey, Catherine C.; Levine, Zachary H.; Pintar, Adam L.; Hwang, Jeeseong; Allen, David W.

2016-01-01

The National Institute of Standards and Technology (NIST) has maintained scales for reflectance and transmittance over several decades. The scales are primarily intended for regular transmittance, mirrors, and solid surface scattering diffusers. The rapidly growing area of optical medical imaging needs a scale for volume scattering of diffuse materials that are used to mimic the optical properties of tissue. Such materials are used as phantoms to evaluate and validate instruments under development intended for clinical use. To address this need, a double-integrating sphere based instrument has been installed to measure the optical properties of tissue-mimicking phantoms. The basic system and methods have been described in previous papers. An important attribute in establishing a viable calibration service is the estimation of measurement uncertainties. The use of custom models and comparisons with other established scales enabled uncertainty measurements. Here, we describe the continuation of those efforts to advance the understanding of the uncertainties through two independent measurements: the bidirectional reflectance distribution function and the bidirectional transmittance distribution function of a commercially available solid biomedical phantom. A Monte Carlo-based model is used and the resulting optical properties are compared to the values provided by the phantom manufacturer. PMID:27453623

In vivo soft tissue differentiation by diffuse reflectance spectroscopy: preliminary results

NASA Astrophysics Data System (ADS)

Zam, Azhar; Stelzle, Florian; Tangermann-Gerk, Katja; Adler, Werner; Nkenke, Emeka; Neukam, Friedrich Wilhelm; Schmidt, Michael; Douplik, Alexandre

Remote laser surgery does not provide haptic feedback to operate layer by layer and preserve vulnerable anatomical structures like nerve tissue or blood vessels. The aim of this study is identification of soft tissue in vivo by diffuse reflectance spectroscopy to set the base for a feedback control system to enhance nerve preservation in oral and maxillofacial laser surgery. Various soft tissues can be identified by diffuse reflectance spectroscopy in vivo. The results may set the base for a feedback system to prevent nerve damage during oral and maxillofacial laser surgery.

Assessment of the biophysical characteristics of rangeland community using scatterometer and optical measurements

NASA Technical Reports Server (NTRS)

Kanemasu, E. T.; Asrar, Ghassem; Myneni, Ranga; Martin, Robert, Jr.; Burnett, R. Bruce

1987-01-01

Research activities for the following study areas are summarized: single scattering of parallel direct and axially symmetric diffuse solar radiation in vegetative canopies; the use of successive orders of scattering approximations (SOSA) for treating multiple scattering in a plant canopy; reflectance of a soybean canopy using the SOSA method; and C-band scatterometer measurements of the Konza tallgrass prairie.

Preliminary assessments of portable color spectrophotometer measurements of cotton color

USDA-ARS?s Scientific Manuscript database

Cotton in the U.S. is classified for color with the Uster® High Volume Instrument (HVI), using the parameters Rd (diffuse reflectance) and +b (yellowness). It has been reported that some cotton bales, especially those transported overseas, appear to have changed significantly in color from their in...

Clinical system model for monitoring the physiological status of jaundice by extracting bilirubin components from skin diffuse reflectance spectra

NASA Astrophysics Data System (ADS)

Kumar, Alla S.; Clark, Joseph; Beyette, Fred R., Jr.

2009-02-01

Neonatal jaundice is a medical condition which occurs in newborns as a result of an imbalance between the production and elimination of bilirubin. The excess bilirubin in the blood stream diffuses into the surrounding tissue leading to a yellowing of the skin. As the bilirubin levels rise in the blood stream, there is a continuous exchange between the extra vascular bilirubin and bilirubin in the blood stream. Exposure to phototherapy alters the concentration of bilirubin in the vascular and extra vascular regions by causing bilirubin in the skin layers to be broken down. Thus, the relative concentration of extra vascular bilirubin is reduced leading to a diffusion of bilirubin out of the vascular region. Diffuse reflectance spectra from human skin contains physiological and structural information of the skin and nearby tissue. A diffuse reflectance spectrum must be captured before and after blanching in order to isolate the intravascular and extra vascular bilirubin. A new mathematical model is proposed with extra vascular bilirubin concentration taken into consideration along with other optical parameters in defining the diffuse reflectance spectrum from human skin. A nonlinear optimization algorithm has been adopted to extract the optical properties (including bilirubin concentration) from the skin reflectance spectrum. The new system model and nonlinear algorithm have been combined to enable extraction of Bilirubin concentrations within an average error of 10%.

Heat Diffusion in Gases, Including Effects of Chemical Reaction

NASA Technical Reports Server (NTRS)

Hansen, C. Frederick

1960-01-01

The diffusion of heat through gases is treated where the coefficients of thermal conductivity and diffusivity are functions of temperature. The diffusivity is taken proportional to the integral of thermal conductivity, where the gas is ideal, and is considered constant over the temperature interval in which a chemical reaction occurs. The heat diffusion equation is then solved numerically for a semi-infinite gas medium with constant initial and boundary conditions. These solutions are in a dimensionless form applicable to gases in general, and they are used, along with measured shock velocity and heat flux through a shock reflecting surface, to evaluate the integral of thermal conductivity for air up to 5000 degrees Kelvin. This integral has the properties of a heat flux potential and replaces temperature as the dependent variable for problems of heat diffusion in media with variable coefficients. Examples are given in which the heat flux at the stagnation region of blunt hypersonic bodies is expressed in terms of this potential.

Cleanliness evaluation of rough surfaces with diffuse IR reflectance

NASA Technical Reports Server (NTRS)

Pearson, L. H.

1995-01-01

Contamination on bonding surfaces has been determined to be a primary cause for degraded bond strength in certain solid rocket motor bondlines. Hydrocarbon and silicone based organic contaminants that are airborne or directly introduced to a surface are a significant source of contamination. Diffuse infrared (IR) reflectance has historically been used as an effective technique for detection of organic contaminants, however, common laboratory methods involving the use of a Fourier transform IR spectrometer (FTIR) are impractical for inspecting the large bonding surface areas found on solid rocket motors. Optical methods involving the use of acousto-optic tunable filters and fixed bandpass optical filters are recommended for increased data acquisition speed. Testing and signal analysis methods are presented which provide for simultaneous measurement of contamination concentration and roughness level on rough metal surfaces contaminated with hydrocarbons.

Evaluation of apparent viscosity of Para rubber latex by diffuse reflection near-infrared spectroscopy.

PubMed

Sirisomboon, Panmanas; Chowbankrang, Rawiphan; Williams, Phil

2012-05-01

Near-infrared spectroscopy in diffuse reflection mode was used to evaluate the apparent viscosity of Para rubber field latex and concentrated latex over the wavelength range of 1100 to 2500 nm, using partial least square regression (PLSR). The model with ten principal components (PCs) developed using the raw spectra accurately predicted the apparent viscosity with correlation coefficient (r), standard error of prediction (SEP), and bias of 0.974, 8.6 cP, and -0.4 cP, respectively. The ratio of the SEP to the standard deviation (RPD) and the ratio of the SEP to the range (RER) for the prediction were 4.4 and 16.7, respectively. Therefore, the model can be used for measurement of the apparent viscosity of field latex and concentrated latex in quality assurance and process control in the factory.

Multivariate analysis of nystatin and metronidazole in a semi-solid matrix by means of diffuse reflectance NIR spectroscopy and PLS regression.

PubMed

Baratieri, Sabrina C; Barbosa, Juliana M; Freitas, Matheus P; Martins, José A

2006-01-23

A multivariate method of analysis of nystatin and metronidazole in a semi-solid matrix, based on diffuse reflectance NIR measurements and partial least squares regression, is reported. The product, a vaginal cream used in the antifungal and antibacterial treatment, is usually, quantitatively analyzed through microbiological tests (nystatin) and HPLC technique (metronidazole), according to pharmacopeial procedures. However, near infrared spectroscopy has demonstrated to be a valuable tool for content determination, given the rapidity and scope of the method. In the present study, it was successfully applied in the prediction of nystatin (even in low concentrations, ca. 0.3-0.4%, w/w, which is around 100,000 IU/5g) and metronidazole contents, as demonstrated by some figures of merit, namely linearity, precision (mean and repeatability) and accuracy.

Design of bifunctional metasurface based on independent control of transmission and reflection.

PubMed

Zhuang, Yaqiang; Wang, Guangming; Cai, Tong; Zhang, Qingfeng

2018-02-05

Multifunctional metasurface integrating different functions can significantly save the occupied space, although most of bifunctional metasurfaces reported to date only control the wave in either reflection or transmission regime. In this paper, we propose a scheme that allows one to independently control the reflection and transmission wavefront under orthogonal polarizations. For demonstration, we design a bifunctional metasurface that simultaneously realizes a diffusion reflection and a focusing transmission. The diffusion reflection is realized using a random phase distribution, which was implemented by randomly arranging two basic coding unit cells with the aid of an ergodic algorithm. Meanwhile, the hyperbolic phase distribution was designed to realize the focusing functionality in the transmission regime. To further show the potential applications, a high-gain lens antenna was designed by assembling the proposed metasurface with a proper feed. Both simulation and measurement results have been carried out, and the agreement between the two results demonstrates the validity of the performance as expected. The backward scattering can be reduced more than 5 dB within 6.4-10 GHz compared with the metallic plate. Moreover, the lens antenna has a gain of 20 dB (with around 13 dB enhancement in comparison with the bare feeding antenna) and an efficiency of 32.5%.

An instrument for the geometric attributes of metallic appearance.

PubMed

Christie, J S

1969-09-01

With the use of a greater variety of metals and methods of finishing them, an increasing need to measure metallic appearance has developed in the automotive industry. A simple and easy to operate instrument has been designed to measure the geometric characteristics of reflectance related to metallic appearance. These are specular reflectance, distinctness of image, haze, and diffuseness. A series of selected aluminum and stainless steel specimens has been used to test the performance of the new instrument and of older devices with which it has been compared. Functionally, the new instrument combines features of the Distinctness of ReflectedImage (DORI)meter designed by Tingle, and the abridged goniophotometer designed by Tingle and George. The design and operation of the new instrument have been simplified by use of multiple receptor apertures with optical fiber light collectors. The measurement of a wide range of metal appearance characteristic has thus been achieved with mechanical and electrical circuit simplicity.

X-ray reflectivity measurement of interdiffusion in metallic multilayers during rapid heating

PubMed Central

Liu, J. P.; Kirchhoff, J.; Zhou, L.; Zhao, M.; Grapes, M. D.; Dale, D. S.; Tate, M. D.; Philipp, H. T.; Gruner, S. M.; Weihs, T. P.; Hufnagel, T. C.

2017-01-01

A technique for measuring interdiffusion in multilayer materials during rapid heating using X-ray reflectivity is described. In this technique the sample is bent to achieve a range of incident angles simultaneously, and the scattered intensity is recorded on a fast high-dynamic-range mixed-mode pixel array detector. Heating of the multilayer is achieved by electrical resistive heating of the silicon substrate, monitored by an infrared pyrometer. As an example, reflectivity data from Al/Ni heated at rates up to 200 K s−1 are presented. At short times the interdiffusion coefficient can be determined from the rate of decay of the reflectivity peaks, and it is shown that the activation energy for interdiffusion is consistent with a grain boundary diffusion mechanism. At longer times the simple analysis no longer applies because the evolution of the reflectivity pattern is complicated by other processes, such as nucleation and growth of intermetallic phases. PMID:28664887

X-ray reflectivity measurement of interdiffusion in metallic multilayers during rapid heating

DOE PAGES

Liu, J. P.; Kirchhoff, J.; Zhou, L.; ...

2017-06-15

A technique for measuring interdiffusion in multilayer materials during rapid heating using X-ray reflectivity is described. In this technique the sample is bent to achieve a range of incident angles simultaneously, and the scattered intensity is recorded on a fast high-dynamic-range mixed-mode pixel array detector. Heating of the multilayer is achieved by electrical resistive heating of the silicon substrate, monitored by an infrared pyrometer. As an example, reflectivity data from Al/Ni heated at rates up to 200 K s -1 are presented. At short times the interdiffusion coefficient can be determined from the rate of decay of the reflectivity peaks,more » and it is shown that the activation energy for interdiffusion is consistent with a grain boundary diffusion mechanism. At longer times the simple analysis no longer applies because the evolution of the reflectivity pattern is complicated by other processes, such as nucleation and growth of intermetallic phases.« less

Bio-inspired, sub-wavelength surface structures for ultra-broadband, omni-directional anti-reflection in the mid and far IR.

PubMed

Gonzalez, Federico Lora; Gordon, Michael J

2014-06-02

Quasi-ordered moth-eye arrays were fabricated in Si using a colloidal lithography method to achieve highly efficient, omni-directional transmission of mid and far infrared (IR) radiation. The effect of structure height and aspect ratio on transmittance and scattering was explored experimentally and modeled quantitatively using effective medium theory. The highest aspect ratio structures (AR = 9.4) achieved peak transmittance of 98%, with >85% transmission for λ = 7-30 μm. A detailed photon balance was constructed by measuring transmission, forward scattering, specular reflection and diffuse reflection to quantify optical losses due to near-field effects. In addition, angle-dependent transmission measurements showed that moth-eye structures provide superior anti-reflective properties compared to unstructured interfaces over a wide angular range (0-60° incidence). The colloidal lithography method presented here is scalable and substrate-independent, providing a general approach to realize moth-eye structures and anti-reflection in many IR-compatible material systems.

Thermal diffusivity determination using heterodyne phase insensitive transient grating spectroscopy

NASA Astrophysics Data System (ADS)

Dennett, Cody A.; Short, Michael P.

2018-06-01

The elastic and thermal transport properties of opaque materials may be measured using transient grating spectroscopy (TGS) by inducing and monitoring periodic excitations in both reflectivity and surface displacement. The "phase grating" response encodes both properties of interest, but complicates quantitative analysis by convolving temperature dynamics with surface displacement dynamics. Thus, thermal transport characteristics are typically determined using the "amplitude grating" response to isolate the surface temperature dynamics. However, this signal character requires absolute heterodyne phase calibration and contains no elastic property information. Here, a method is developed by which phase grating TGS measurements may be consistently analyzed to determine thermal diffusivity with no prior knowledge of the expected properties. To demonstrate this ability, the wavelength-dependent 1D effective thermal diffusivity of pure germanium is measured using this type of response and found to be consistent with theoretical predictions made by solving the Boltzmann transport equation. This ability to determine the elastic and thermal properties from a single set of TGS measurements will be particularly advantageous for new in situ implementations of the technique being used to study dynamic materials systems.

Utilizing Diffuse Reflection to Increase the Efficiency of Luminescent Solar Concentrators

NASA Astrophysics Data System (ADS)

Bowser, Seth; Weible, Seth; Solomon, Joel; Schrecengost, Jonathan; Wittmershaus, Bruce

A luminescent solar concentrator (LSC) consists of a high index solid plate containing a fluorescent material that converts sunlight into fluorescence. Utilizing total internal reflection, the LSC collects and concentrates the fluorescence at the plate's edges where it is converted into electricity via photovoltaic solar cells. The lower production costs of LSCs make them an attractive alternative to photovoltaic solar cells. To optimize an LSC's efficiency, a white diffusive surface (background) is positioned behind it. The background allows sunlight transmitted in the first pass to be reflected back through the LSC providing a second chance for absorption. Our research examines how the LSC's performance is affected by changing the distance between the white background and the LSC. An automated linear motion apparatus was engineered to precisely measure this distance and the LSC's electrical current, simultaneously. LSC plates, with and without the presence of fluorescent material and in an isolated environment, showed a maximum current at a distance greater than zero. Further experimentation has proved that the optimal distance results from the background's optical properties and how the reflected light enters the LSC. This material is based upon work supported by the National Science Foundation under Grant Number NSF-ECCS-1306157.

Measuring joint cartilage thickness using reflectance spectroscopy non-invasively and in real-time

NASA Astrophysics Data System (ADS)

Canpolat, Murat; Denkceken, Tuba; Karagol, Cosar; Aydin, Ahmet T.

2011-03-01

Joint cartilage thickness has been estimated using spatially resolved steady-state reflectance spectroscopy noninvasively and in-real time. The system consists of a miniature UV-VIS spectrometer, a halogen tungsten light source, and an optical fiber probe with six 400 um diameter fibers. The first fiber was used to deliver the light to the cartilage and the other five were used to detect back-reflected diffused light. Distances from the detector fibers to the source fiber were 0.8 mm, 1.6 mm, 2.4 mm, 3.2 mm and 4 mm. Spectra of back-reflected diffused light were taken on 40 bovine patella cartilages. The samples were grouped into four; the first group was the control group with undamaged cartilages, in the 2nd, 3rd and 4th groups cartilage thickness was reduced approximately 25%, 50% and 100%, respectively. A correlation between cartilage thicknesses and hemoglobin absorption of light in the wavelength range of 500 nm- 600 nm for source-detector pairs was found. The proposed system with an optical fiber probe less than 4 mm in diameter has the potential for cartilage thickness assessment through an arthroscopy channel in real-time without damaging the cartilage.

An Experimental Study of Diffusivity of Technetium-99 in Hanford Vadose Zone Sediments

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mattigod, Shas V.; Bovaird, Chase C.; Wellman, Dawn M.

2012-11-01

One of the methods being considered at the Hanford site in Washington for safely disposing of low-level radioactive wastes (LLW) is to encase the waste in concrete and entomb the packages in the Hanford vadose zone sediments. The current plan for waste isolation consists of stacking low-level waste packages on a trench floor, surrounding the stacks with reinforced steel, and encasing these packages with concrete. Any failure of the concrete encasement may result in water intrusion and consequent mobilization of radionuclides from the waste packages. The mobilized radionuclides may escape from the encased concrete by mass flow and/or diffusion andmore » move into the surrounding subsurface sediments. It is therefore necessary to conduct an assessment of the performance of the concrete encasement structure and the surrounding soil’s ability to retard radionuclide migration. The retardation factors for radionuclides contained in the waste packages can be determined from measurements of diffusion coefficients for these contaminants through concrete and fill material. Because of their anionic nature in aqueous solutions, the radionuclides, 99Tc and 129I were identified as long-term dose contributors in LLW. The leachability and/or diffusion of these radionuclide species must be measured in order to assess the long-term performance of waste grouts when contacted with vadose-zone porewater or groundwater. To measure the diffusivity, a set of experiments were conducted using 99Tc-spiked concrete (with 0 and 4% metallic iron additions) in contact with unsaturated soil half-cells that reflected the typical moisture contents of Hanford vadose zone sediments. The 99Tc diffusion profiles in the soil half cells were measured after a time lapse of ~1.9 yr. Using the concentration profiles, the 99Tc diffusivity coefficients were calculated based on Fick’s Second Law.« less

A total internal reflection-fluorescence correlation spectroscopy setup with pulsed diode laser excitation

NASA Astrophysics Data System (ADS)

Weger, Lukas; Hoffmann-Jacobsen, Kerstin

2017-09-01

Fluorescence correlation spectroscopy (FCS) measures fluctuations in a (sub-)femtoliter volume to analyze the diffusive behavior of fluorescent particles. This highly sensitive method has proven to be useful for the analysis of dynamic biological systems as well as in chemistry, physics, and material sciences. It is routinely performed with commercial fluorescence microscopes, which provide a confined observation volume by the confocal technique. The evanescent wave of total internal reflectance (TIR) is used in home-built systems to permit a surface sensitive FCS analysis. We present a combined confocal and TIR-FCS setup which uses economic low-power pulsed diode lasers for excitation. Excitation and detection are coupled to time-correlated photon counting hardware. This allows simultaneous fluorescence lifetime and FCS measurements in a surface-sensitive mode. Moreover, the setup supports fluorescence lifetime correlation spectroscopy at surfaces. The excitation can be easily switched between TIR and epi-illumination to compare the surface properties with those in liquid bulk. The capabilities of the presented setup are demonstrated by measuring the diffusion coefficients of a free dye molecule, a labeled polyethylene glycol, and a fluorescent nanoparticle in confocal as well as in TIR-FCS.

Refractometry studies of the optical properties of polymer films and the development of polymer coated refractive index sensors

NASA Astrophysics Data System (ADS)

Saunders, John Edward

Sensors for real-time monitoring of environmental contaminants are essential for protecting ecosystems and human health. Refractive index sensing is a non-selective technique that can be used to measure almost any analyte. Miniaturized refractive index sensors, such as silicon-on-insulator (SOI) microring resonators are one possible platform, but require coatings selective to the analytes of interest. A homemade prism refractometer is reported and used to characterize the interactions between polymer films and liquid or vapour-phase analytes. A camera was used to capture both Fresnel reflection and total internal reflection within the prism. For thin-films (d = 10 μm - 100 μm), interference fringes were also observed. Fourier analysis of the interferogram allowed for simultaneous extraction of the average refractive index and film thickness with accuracies of Δn = 1-7 x10-4 and Δd < 3-5%. The refractive indices of 29 common organic solvents as well as aqueous solutions of sodium chloride, sucrose, ethylene glycol, glycerol, and dimethylsulfoxide were measured at λ = 1550 nm. These measurements will be useful for future calibrations of near-infrared refractive index sensors. A mathematical model is presented, where the concentration of analyte adsorbed in a film can be calculated from the refractive index and thickness changes during uptake. This model can be used with Fickian diffusion models to measure the diffusion coefficients through the bulk film and at the film-substrate interface. The diffusion of water and other organic solvents into SU-8 epoxy was explored using refractometry and the diffusion coefficient of water into SU-8 is presented. Exposure of soft baked SU-8 films to acetone, acetonitrile and methanol resulted in rapid delamination. The diffusion of volatile organic compound (VOC) vapours into polydimethylsiloxane and polydimethyl-co-polydiphenylsiloxane polymers was also studied using refractometry. Diffusion and partition coefficients are reported for several analytes. As a model system, polydimethyl-co-diphenylsiloxane films were coated onto SOI microring resonators. After the development of data acquisition software, coated devices were exposed to VOCs and the refractive index response was assessed. More studies with other polymers are required to test the viability of this platform for environmental sensing applications.

Mapping diffuse photosynthetically active radiation from satellite data in Thailand

NASA Astrophysics Data System (ADS)

Choosri, P.; Janjai, S.; Nunez, M.; Buntoung, S.; Charuchittipan, D.

2017-12-01

In this paper, calculation of monthly average hourly diffuse photosynthetically active radiation (PAR) using satellite data is proposed. Diffuse PAR was analyzed at four stations in Thailand. A radiative transfer model was used for calculating the diffuse PAR for cloudless sky conditions. Differences between the diffuse PAR under all sky conditions obtained from the ground-based measurements and those from the model are representative of cloud effects. Two models are developed, one describing diffuse PAR only as a function of solar zenith angle, and the second one as a multiple linear regression with solar zenith angle and satellite reflectivity acting linearly and aerosol optical depth acting in logarithmic functions. When tested with an independent data set, the multiple regression model performed best with a higher coefficient of variance R2 (0.78 vs. 0.70), lower root mean square difference (RMSD) (12.92% vs. 13.05%) and the same mean bias difference (MBD) of -2.20%. Results from the multiple regression model are used to map diffuse PAR throughout the country as monthly averages of hourly data.

Noncontact true temperature measurement, 2

NASA Technical Reports Server (NTRS)

Lee, Mark C.; Allen, James L.

1988-01-01

A laser pyrometer was developed for acquiring the true temperature of a levitated sample. The reflectivity is measured by first expanding the laser beam to cover the entire cross-sectional surface of the diffuse target. The reflectivity calibration of this system is determined from the surface emissivity of a target with a blackbody cavity. The emissivity of the real target can then be calculated. The overall system constant is obtained by passively measuring the radiance of the blackbody cavity (emissivity = 1.0) at a known, arbitrary temperature. Since the photosensor used is highly linear over the entire operating temperature range, the true temperature of the target can then be computed. The latest results available from this on-going research indicate that true temperatures thus obtained are in very good quantitative agreement with thermocouple measured temperatures.

Regression-based model of skin diffuse reflectance for skin color analysis

NASA Astrophysics Data System (ADS)

Tsumura, Norimichi; Kawazoe, Daisuke; Nakaguchi, Toshiya; Ojima, Nobutoshi; Miyake, Yoichi

2008-11-01

A simple regression-based model of skin diffuse reflectance is developed based on reflectance samples calculated by Monte Carlo simulation of light transport in a two-layered skin model. This reflectance model includes the values of spectral reflectance in the visible spectra for Japanese women. The modified Lambert Beer law holds in the proposed model with a modified mean free path length in non-linear density space. The averaged RMS and maximum errors of the proposed model were 1.1 and 3.1%, respectively, in the above range.

Design and validation of a diffuse reflectance and spectroscopic microendoscope with poly(dimethylsioxane)-based phantoms

PubMed Central

Greening, Gage J.; Powless, Amy J.; Hutcheson, Joshua A.; Prieto, Sandra P.; Majid, Aneeka A.; Muldoon, Timothy J.

2015-01-01

Many cases of epithelial cancer originate in basal layers of tissue and are initially undetected by conventional microendoscopy techniques. We present a bench-top, fiber-bundle microendoscope capable of providing high resolution images of surface cell morphology. Additionally, the microendoscope has the capability to interrogate deeper into material by using diffuse reflectance and broadband diffuse reflectance spectroscopy. The purpose of this multimodal technique was to overcome the limitation of microendoscopy techniques that are limited to only visualizing morphology at the tissue or cellular level. Using a custom fiber optic probe, high resolution surface images were acquired using topical proflavine to fluorescently stain non-keratinized epithelia. A 635 nm laser coupled to a 200 μm multimode fiber delivers light to the sample and the diffuse reflectance signal was captured by a 1 mm image guide fiber. Finally, a tungsten-halogen lamp coupled to a 200 μm multimode fiber delivers broadband light to the sample to acquire spectra at source-detector separations of 374, 729, and 1051 μm. To test the instrumentation, a high resolution proflavine-induced fluorescent image of resected healthy mouse colon was acquired. Additionally, five monolayer poly(dimethylsiloxane)-based optical phantoms with varying absorption and scattering properties were created to acquire diffuse reflectance profiles and broadband spectra. PMID:25983372

Influence of Acoustic Reflection on the Inertial Cavitation Dose in a Franz Diffusion Cell.

PubMed

Robertson, Jeremy; Becker, Sid

2018-05-01

The exposure of the skin to low-frequency (20-100 kHz) ultrasound is a well-established method for increasing its permeability to drugs. The mechanism underlying this permeability increase has been found to be inertial cavitation within the coupling fluid. This study investigated the influence of acoustic reflections on the inertial cavitation dose during low-frequency (20 kHz) exposure in an in vitro skin sonoporation setup. This investigation was conducted using a passive cavitation detector that monitored the broadband noise emission within a modified Franz diffusion cell. Two versions of this diffusion cell were employed. One version had acoustic conditions that were similar to those of a standard Franz diffusion cell surrounded by air, whereas the second was designed to greatly reduce the acoustic reflection by submerging the diffusion cell in a water bath. The temperature of the coupling fluid in both setups was controlled using a novel thermoelectric cooling system. At an ultrasound intensity of 13.6 W/cm 2 , the median inertial cavitation dose when the acoustic reflections were suppressed, was found to be only about 15% lower than when reflections were not suppressed. Copyright © 2018 World Federation for Ultrasound in Medicine and Biology. Published by Elsevier Inc. All rights reserved.

Design and validation of a diffuse reflectance and spectroscopic microendoscope with poly(dimethylsiloxane)-based phantoms

NASA Astrophysics Data System (ADS)

Greening, Gage J.; Powless, Amy J.; Hutcheson, Joshua A.; Prieto, Sandra P.; Majid, Aneeka A.; Muldoon, Timothy J.

2015-03-01

Many cases of epithelial cancer originate in basal layers of tissue and are initially undetected by conventional microendoscopy techniques. We present a bench-top, fiber-bundle microendoscope capable of providing high resolution images of surface cell morphology. Additionally, the microendoscope has the capability to interrogate deeper into material by using diffuse reflectance and broadband diffuse reflectance spectroscopy. The purpose of this multimodal technique was to overcome the limitation of microendoscopy techniques that are limited to only visualizing morphology at the tissue or cellular level. Using a custom fiber optic probe, high resolution surface images were acquired using topical proflavine to fluorescently stain non-keratinized epithelia. A 635 nm laser coupled to a 200 μm multimode fiber delivers light to the sample and the diffuse reflectance signal was captured by a 1 mm image guide fiber. Finally, a tungsten-halogen lamp coupled to a 200 μm multimode fiber delivers broadband light to the sample to acquire spectra at source-detector separations of 374, 729, and 1051 μm. To test the instrumentation, a high resolution proflavine-induced fluorescent image of resected healthy mouse colon was acquired. Additionally, five monolayer poly(dimethylsiloxane)-based optical phantoms with varying absorption and scattering properties were created to acquire diffuse reflectance profiles and broadband spectra.

Aerosol Optical Depth Retrievals From High-Resolution Commercial Satellite Imagery Over Areas of High Surface Reflectance

NASA Astrophysics Data System (ADS)

Vincent, D. A.; Nielsen, K. E.; Durkee, P. A.; Reid, J. S.

2005-12-01

The advancement and proliferation of high-resolution commercial imaging satellites presents a new opportunity for overland aerosol characterization. Current aerosol optical depth retrieval methods typically fail over areas with high surface reflectance, such as urban areas and deserts, since the upwelling radiance due to scattering by aerosols is small compared to the radiance resulting from surface reflection. The method proposed here uses shadows cast on the surface to exploit the differences between radiance from the adjacent shaded and unshaded areas of the scene. Shaded areas of the scene are primarily illuminated by diffuse irradiance that is scattered downward from the atmosphere, while unshaded areas are illuminated by both diffuse and direct solar irradiance. The first-order difference between the shaded and unshaded areas is the direct component. Given uniform surface reflectance for the shaded and unshaded areas, the difference in reflected radiance measured by a satellite sensor is related to the direct transmission of solar radiation and inversely proportional to total optical depth. Using an iterative approach, surface reflectance and mean aerosol reflectance can be partitioned to refine the retrieved total optical depth. Aerosol optical depth can then be determined from its contribution to the total atmospheric optical depth (following correction for molecular Rayleigh scattering). Intitial results based on QuickBird imagery and AERONET data collected during the United Arab Emirates Unified Aerosol Experiment (UAE2) indicate that aerosol optical depth retrievals are possible in the visible and near-infrared region with an accuracy of ~0.04.

Reflectance spectroscopy of pigmented cutaneous benign and malignant lesions

NASA Astrophysics Data System (ADS)

Borisova, E.; Jeliazkova, Al.; Pavlova, E.; Troyanova, P.; Kundurdjiev, T.; Pavlova, P.; Avramov, L.

2014-10-01

For the DRS measurements of skin benign, dysplastic and malignant lesions in vivo we applied halogen lamp (LS-1, OceanOptics Inc, Dunedin, Fl, USA) as a continuous light source in the region of 400-900 nm, optical probe (6+1 fibers) for the delivery of illumination and diffuse reflected light from the skin investigated and microspectrometer USB4000 (OceanOptics Inc., Dunedin, Fl, USA) for a storage and display of the spectra detected. As a diffuse reflectance standard Spectralon® plate was used to calibrate the spectrometer. The reflectance spectra obtained from normal skin in identical anatomic sites of different patients have similar spectral shape features, slightly differ by the reflectance intensity at different wavelengths, depending on the particular patient' skin phototype. One could find diagnostically important spectral features, related to specific intensity changes for a given wavelength due to specific pigments appearance, slope changes by value and sign for the reflectance spectra curves in a specific spectral range, disappearance or manifestation of minima, related to hemoglobin absorption at 410-420 nm, 543, 575 nm. Based on the observed peculiarities multispectral analysis of the reflectance spectra of the different lesions was used and diagnostically specific features are found. Discrimination using the DRS data obtained between benign compound and dermal nevi (45 cases), dysplastic nevi (17 cases) and pigmented malignant melanoma (41 cases) lesions is achieved with a diagnostic accuracy of 96 % for the benign nevi vs. MM, and 90 % for the dysplastic nevi vs. MM.

Atmospheric optical depth effects on angular anisotropy of plant canopy reflectance

NASA Technical Reports Server (NTRS)

Deering, Donald W.; Eck, Thomas F.

1987-01-01

The effects of varying atmospheric aerosol optical depth on the bidirectional reflectance distribution of vegetation canopies is investigated. The reflectance distributions of two pasture grass canopies and one soya bean canopy under different sky irradiance distributions were measured, and the data were analyzed in the visible and IR spectral bands. It is observed that, for the pasture grass canopies, the change in reflectance is due to the percentage of shadowed area viewed by the sensor, and for the soya bean, the specular reflection effect and increased diffuse irradiance penetration into the canopy cause reflectance changes. It is detected that the reflectivity for the soya bean canopy on a hazy day is lower than on a clear day; however, the opposite change is observed for the pasture grass. It is also detected that the normalized difference vegetation index values differ under clear and hazy conditions for the same vegetation canopy conditions.

The reflectance of Ames 24E, Infrablack, and Martin black. [anodizing coatings for far-infrared space telescopes

NASA Technical Reports Server (NTRS)

Smith, Sheldon M.

1989-01-01

Results are reported from measurements of the specular reflectances (SRs) and bidirectional reflectance distribution functions (BRDFs) of three black optical coatings in the FIR wavelength range. The nonspecular reflectometer apparatus described by Smith (1984) is employed, and the data are presented in tables and graphs and discussed in detail. It is found that Ames 24E has an FIR SR one order of magnitude lower than that of Martin black (MB), with BRDF values characteristic of a nearly Lambertian surface, while Infrablack has SR two orders lower than MB and a specular-diffuse surface; MB itself has a very specular surface.

Speckle contrast diffuse correlation tomography of complex turbid medium flow

DOE Office of Scientific and Technical Information (OSTI.GOV)

Huang, Chong; Irwin, Daniel; Lin, Yu

2015-07-15

Purpose: Developed herein is a three-dimensional (3D) flow contrast imaging system leveraging advancements in the extension of laser speckle contrast imaging theories to deep tissues along with our recently developed finite-element diffuse correlation tomography (DCT) reconstruction scheme. This technique, termed speckle contrast diffuse correlation tomography (scDCT), enables incorporation of complex optical property heterogeneities and sample boundaries. When combined with a reflectance-based design, this system facilitates a rapid segue into flow contrast imaging of larger, in vivo applications such as humans. Methods: A highly sensitive CCD camera was integrated into a reflectance-based optical system. Four long-coherence laser source positions were coupledmore » to an optical switch for sequencing of tomographic data acquisition providing multiple projections through the sample. This system was investigated through incorporation of liquid and solid tissue-like phantoms exhibiting optical properties and flow characteristics typical of human tissues. Computer simulations were also performed for comparisons. A uniquely encountered smear correction algorithm was employed to correct point-source illumination contributions during image capture with the frame-transfer CCD and reflectance setup. Results: Measurements with scDCT on a homogeneous liquid phantom showed that speckle contrast-based deep flow indices were within 12% of those from standard DCT. Inclusion of a solid phantom submerged below the liquid phantom surface allowed for heterogeneity detection and validation. The heterogeneity was identified successfully by reconstructed 3D flow contrast tomography with scDCT. The heterogeneity center and dimensions and averaged relative flow (within 3%) and localization were in agreement with actuality and computer simulations, respectively. Conclusions: A custom cost-effective CCD-based reflectance 3D flow imaging system demonstrated rapid acquisition of dense boundary data and, with further studies, a high potential for translatability to real tissues with arbitrary boundaries. A requisite correction was also found for measurements in the fashion of scDCT to recover accurate speckle contrast of deep tissues.« less

Measurement of fluorophore concentrations and fluorescence quantum yield in tissue-simulating phantoms using three diffusion models of steady-state spatially resolved fluorescence.

PubMed

Diamond, Kevin R; Farrell, Thomas J; Patterson, Michael S

2003-12-21

Steady-state diffusion theory models of fluorescence in tissue have been investigated for recovering fluorophore concentrations and fluorescence quantum yield. Spatially resolved fluorescence, excitation and emission reflectance Carlo simulations, and measured using a multi-fibre probe on tissue-simulating phantoms containing either aluminium phthalocyanine tetrasulfonate (AlPcS4), Photofrin meso-tetra-(4-sulfonatophenyl)-porphine dihydrochloride The accuracy of the fluorophore concentration and fluorescence quantum yield recovered by three different models of spatially resolved fluorescence were compared. The models were based on: (a) weighted difference of the excitation and emission reflectance, (b) fluorescence due to a point excitation source or (c) fluorescence due to a pencil beam excitation source. When literature values for the fluorescence quantum yield were used for each of the fluorophores, the fluorophore absorption coefficient (and hence concentration) at the excitation wavelength (mu(a,x,f)) was recovered with a root-mean-square accuracy of 11.4% using the point source model of fluorescence and 8.0% using the more complicated pencil beam excitation model. The accuracy was calculated over a broad range of optical properties and fluorophore concentrations. The weighted difference of reflectance model performed poorly, with a root-mean-square error in concentration of about 50%. Monte Carlo simulations suggest that there are some situations where the weighted difference of reflectance is as accurate as the other two models, although this was not confirmed experimentally. Estimates of the fluorescence quantum yield in multiple scattering media were also made by determining mu(a,x,f) independently from the fitted absorption spectrum and applying the various diffusion theory models. The fluorescence quantum yields for AlPcS4 and TPPS4 were calculated to be 0.59 +/- 0.03 and 0.121 +/- 0.001 respectively using the point source model, and 0.63 +/- 0.03 and 0.129 +/- 0.002 using the pencil beam excitation model. These results are consistent with published values.

BRDF profile of Tyvek and its implementation in the Geant4 simulation toolkit.

PubMed

Nozka, Libor; Pech, Miroslav; Hiklova, Helena; Mandat, Dusan; Hrabovsky, Miroslav; Schovanek, Petr; Palatka, Miroslav

2011-02-28

Diffuse and specular characteristics of the Tyvek 1025-BL material are reported with respect to their implementation in the Geant4 Monte Carlo simulation toolkit. This toolkit incorporates the UNIFIED model. Coefficients defined by the UNIFIED model were calculated from the bidirectional reflectance distribution function (BRDF) profiles measured with a scatterometer for several angles of incidence. Results were amended with profile measurements made by a profilometer.

Wavelength Coded Image Transmission and Holographic Optical Elements.

DTIC Science & Technology

1984-08-20

system has been designed and built for transmitting images of diffusely reflecting objects through optical fibers and displaying those images at a...passive components at the end of a fiber-optic designed to transmit high-resolution images of diffusely imaging system as described in this paper... designing a system for viewing diffusely reflecting The authors are with University of Minnesota. Electrical Engi- objects, one must consider that a

A new in-situ method to determine the apparent gas diffusion coefficient of soils

NASA Astrophysics Data System (ADS)

Laemmel, Thomas; Paulus, Sinikka; Schack-Kirchner, Helmer; Maier, Martin

2015-04-01

Soil aeration is an important factor for the biological activity in the soil and soil respiration. Generally, gas exchange between soil and atmosphere is assumed to be governed by diffusion and Fick's Law is used to describe the fluxes in the soil. The "apparent soil gas diffusion coefficient" represents the proportional factor between the flux and the gas concentration gradient in the soil and reflects the ability of the soil to "transport passively" gases through the soil. One common way to determine this coefficient is to take core samples in the field and determine it in the lab. Unfortunately this method is destructive and needs laborious field work and can only reflect a small fraction of the whole soil. As a consequence insecurity about the resulting effective diffusivity on the profile scale must remain. We developed a new in-situ method using new gas sampling device, tracer gas and inverse soil gas modelling. The gas sampling device contains several sampling depths and can be easily installed into vertical holes of an auger, which allows for fast installation of the system. At the lower end of the device inert tracer gas is injected continuously. The tracer gas diffuses into the surrounding soil. The resulting distribution of the tracer gas concentrations is used to deduce the diffusivity profile of the soil. For Finite Element Modeling of the gas sampling device/soil system the program COMSOL is used. We will present the results of a field campaign comparing the new in-situ method with lab measurements on soil cores. The new sampling pole has several interesting advantages: it can be used in-situ and over a long time; so it allows following modifications of diffusion coefficients in interaction with rain but also vegetation cycle and wind.

Cell wall compositional changes during incubation of plant roots measured by mid-infrared diffuse reflectance spectroscopy and fiber analysis

USDA-ARS?s Scientific Manuscript database

Plant roots, particularly the constituents of root cell walls (hemicellulose, cellulose and lignin), are important contributors to soil organic matter. Little is known about the cell wall composition of many important crop species or compositional changes as roots decay. The objectives of this stu...

A Lab Experience to Illustrate the Physicochemical Principles of Detergency

ERIC Educational Resources Information Center

Poce-Fatou, J. A.; Bethencourt-Nunez, M.; Moreno, C.; Pinto-Ganfornina, J. J.; Moreno-Dorado, F. J.

2008-01-01

This article presents a lab experience to study detergency from a physicochemical point of view intended for undergraduate students. By means of a simple experimental device, we analyze the influence of the surfactant concentration in both distilled water and tap water. Our method is based on the measurement of diffuse reflectances of polyester…

Syntheses, characterization and nonlinear optical properties of sodium-scandium carbonate Na5Sc(CO3)4·2H2O

NASA Astrophysics Data System (ADS)

Chen, Jie; Luo, Min; Ye, Ning

2014-10-01

A novel nonlinear optical (NLO) material Na5Sc(CO3)4·2H2O has been synthesized under a subcritical hydrothermal condition. The structure is determined by single-crystal X-ray diffraction and further characterized by TG analyses and UV-vis-NIR diffuse reflectance spectrum. It crystallizes in the tetragonal space group P-421c, with a = b = 7.4622(6) Å, C = 11.5928(15) Å. The Second-harmonic generation (SHG) on polycrystalline samples was measured using the Kurtz and Perry technique, which indicated that Na5Sc(CO3)4·2H2O was a phase-matchable material, and its measured SHG coefficient was about 1.8 times as large as that of d36 (KDP). The results from the UV-vis diffuse reflectance spectroscopy study of the powder samples indicated that the short-wavelength absorption edges of Na5Sc(CO3)4·2H2O is about 220 nm, suggesting that this crystal is a promising UV nonlinear optical (NLO) materials.

A non-invasive diffuse reflectance calibration-free method for absolute determination of exogenous biochemicals concentration in biological tissues

NASA Astrophysics Data System (ADS)

Lappa, Alexander V.; Kulikovskiy, Artem N.; Busarov, Oleg G.

2014-03-01

The paper presents a new method for distant non-destructive determination of concentration of light absorbing admixtures in turbid media. In particular, it is intended for non-invasive in vivo control of accumulation in patient tissues of various biochemicals introduced to the patients for chemotherapy, photodynamic therapy or diagnostics. It is require that the admixture absorption spectrum should have a clearly marked peak in the wavelength region where the pure medium one varies regularly. Fluorescence of admixtures is not required. The method uses the local diffuse reflectance spectroscopy with optical fiber probe including one emitting and two reading There are several features in the method: the value to be determined is absolute concentration of admixtures; the method needs no calibration measurements on phantoms; it needs no reference measurements on sample with zero admixture concentration; it uses a two parametric kinetic light propagation model and original algorithms to resolve direct and inverse tasks of radiation transport theory. Experimental testing passed with tissue equivalent phantoms and different admixtures, including a chlorine photosensitizer, showed accuracy under 10% in all cases.

Separation and correlation of structural and magnetic roughness in a Ni thin film by polarized off-specular neutron reflectometry.

PubMed

Singh, Surendra; Basu, Saibal

2009-02-04

Diffuse (off-specular) neutron and x-ray reflectometry has been used extensively for the determination of interface morphology in solids and liquids. For neutrons, a novel possibility is off-specular reflectometry with polarized neutrons to determine the morphology of a magnetic interface. There have been few such attempts due to the lower brilliance of neutron sources, though magnetic interaction of neutrons with atomic magnetic moments is much easier to comprehend and easily tractable theoretically. We have obtained a simple and physically meaningful expression, under the Born approximation, for analyzing polarized diffuse (off-specular) neutron reflectivity (PDNR) data. For the first time PDNR data from a Ni film have been analyzed and separate chemical and magnetic morphologies have been quantified. Also specular polarized neutron reflectivity measurements have been carried out to measure the magnetic moment density profile of the Ni film. The fit to PDNR data results in a longer correlation length for in-plane magnetic roughness than for chemical (structural) roughness. The magnetic interface is smoother than the chemical interface.

In vivo optical characterization of pediatric epileptogenic lesions

NASA Astrophysics Data System (ADS)

Lin, W.-C.; Ragheb, J.; Bhatia, S.; Johnson, Mahlon D.; Sandberg, D.; Fernandez, A.; Morrison, G.; Duchowny, M.; Jayakar, P.

2007-02-01

Epileptogenic lesions and their margins are often difficult to define intraoperatively. We hypothesize that optical spectroscopy can detect unique pathophysiological features of epileptogenic lesions in children and hence differentiate them from normal brain. This hypothesis was tested by comparing the in vivo optical and fluorescence characteristics of epileptogenic brain lesions (non-neoplastic) with those of normal brain. Patients were recruited from those receiving epilepsy surgeries at Miami Children's Hospital. Using a portable spectroscopic system, optical characterization of brain was performed intraoperatively. Fluorescence spectra were measured at 337 nm excitation, and diffuse reflectance spectra were measured between 400 and 850 nm. To date, seven epilepsy patients have been enrolled in the study. A couple interesting trends have been observed in the recorded optical spectra. First, sites within the resection zone, as defined by the intracranial electroencephalogram data, often show higher diffuse reflectance signals than normal sites do. This is especially prominent around 500 nm and between 650 and 850 nm. Secondly, several investigated sites with abnormal electroencephalogram and/or pathology show a unique blue shift in their fluorescence spectra, which is not seen in other cases.

Improvement of laser molecular beam epitaxy grown SrTiO3 thin film properties by temperature gradient modulation growth

NASA Astrophysics Data System (ADS)

Li, Jin Long; Hao, J. H.; Li, Y. R.

2007-09-01

Oxygen diffusion at the SrTiO3/Si interface was analyzed. A method called temperature gradient modulation growth was introduced to control oxygen diffusion at the interface of SrTiO3/Si. Nanoscale multilayers were grown at different temperatures at the initial growing stage of films. Continuous growth of SrTiO3 films was followed to deposit on the grown sacrificial layers. The interface and crystallinity of SrTiO3/Si were investigated by in situ reflection high energy electron diffraction and x-ray diffraction measurements. It has been shown that the modulated multilayers may help suppress the interfacial diffusion, and therefore improve SrTiO3 thin film properties.

Diffuse spreading of inhomogeneities in the ionospheric dusty plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Shalimov, S. L., E-mail: pmsk7@mail.ru; Kozlovsky, A.

2015-08-15

According to results of sounding of the lower ionosphere at altitudes of about 100 km, the duration of radio reflections from sufficiently dense ionized meteor trails, which characterizes their lifetime, can reach a few tens of seconds to several tens of minutes. This is much longer than the characteristic spreading time (on the order of fractions of a second to several seconds) typical in meteor radar measurements. The presence of dust in the lower ionosphere is shown to affect the ambipolar diffusion coefficient, which determines the spreading of plasma inhomogeneities. It is found that the diffusion coefficient depends substantially onmore » the charge and size of dust grains, which allows one to explain the results of ionospheric sounding.« less

To See the World in a Grain of Sand: Recognizing the Origin of Sand Specimens by Diffuse Reflectance Infrared Fourier Transform Spectroscopy and Multivariate Exploratory Data Analysis

ERIC Educational Resources Information Center

Pezzolo, Alessandra De Lorenzi

2011-01-01

The diffuse reflectance infrared Fourier transform (DRIFT) spectra of sand samples exhibit features reflecting their composition. Basic multivariate analysis (MVA) can be used to effectively sort subsets of homogeneous specimens collected from nearby locations, as well as pointing out similarities in composition among sands of different origins.…

A portable device for detecting fruit quality by diffuse reflectance Vis/NIR spectroscopy

NASA Astrophysics Data System (ADS)

Sun, Hongwei; Peng, Yankun; Li, Peng; Wang, Wenxiu

2017-05-01

Soluble solid content (SSC) is a major quality parameter to fruit, which has influence on its flavor or texture. Some researches on the on-line non-invasion detection of fruit quality were published. However, consumers desire portable devices currently. This study aimed to develop a portable device for accurate, real-time and nondestructive determination of quality factors of fruit based on diffuse reflectance Vis/NIR spectroscopy (520-950 nm). The hardware of the device consisted of four units: light source unit, spectral acquisition unit, central processing unit, display unit. Halogen lamp was chosen as light source. When working, its hand-held probe was in contact with the surface of fruit samples thus forming dark environment to shield the interferential light outside. Diffuse reflectance light was collected and measured by spectrometer (USB4000). ARM (Advanced RISC Machines), as central processing unit, controlled all parts in device and analyzed spectral data. Liquid Crystal Display (LCD) touch screen was used to interface with users. To validate its reliability and stability, 63 apples were tested in experiment, 47 of which were chosen as calibration set, while others as prediction set. Their SSC reference values were measured by refractometer. At the same time, samples' spectral data acquired by portable device were processed by standard normalized variables (SNV) and Savitzky-Golay filter (S-G) to eliminate the spectra noise. Then partial least squares regression (PLSR) was applied to build prediction models, and the best predictions results was achieved with correlation coefficient (r) of 0.855 and standard error of 0.6033° Brix. The results demonstrated that this device was feasible to quantitatively analyze soluble solid content of apple.

Monitoring longitudinal changes in irradiated head and neck cancer xenografts using diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Vishwanath, Karthik; Jiang, Shudong; Gunn, Jason R.; Marra, Kayla; Andreozzi, Jacqueline M.; Pogue, Brian W.

2016-02-01

Radiation therapy is often used as the preferred clinical treatment for control of localized head and neck cancer. However, during the course of treatment (6-8 weeks), feedback about functional and/or physiological changes within impacted tissue are not obtained, given the onerous financial and/or logistical burdens of scheduling MRI, PET or CT scans. Diffuse optical sensing is well suited to address this problem since the instrumentation can be made low-cost and portable while still being able to non-invasively provide information about vascular oxygenation in vivo. Here we report results from studies that employed an optical fiber-based portable diffuse reflectance spectroscopy (DRS) system to longitudinally monitor changes in tumor vasculature within two head and neck cancer cell lines (SCC-15 and FaDu) xenografted in the flanks of nude mice, in two separate experiments. Once the tumor volumes were 100mm3, 67% of animals received localized (electron beam) radiation therapy in five fractions (8Gy/day, for 5 days) while 33% of the animals served as controls. DRS measurements were obtained from each animal on each day of treatment and then for two weeks post-treatment. Reflectance spectra were parametrized to extract total hemoglobin concentration and blood oxygen-saturation and the resulting time-trends of optical parameters appear to be dissimilar for the two cell-lines. These findings are also compared to previous animal experiments (using the FaDu line) that were irradiated using a photon beam radiotherapy protocol. These results and implications for the use of fiber-based DRS measurements made at local (irradiated) tumor site as a basis for identifying early radiotherapy-response are presented and discussed.

Mechanisms and behavioural functions of structural coloration in cephalopods

PubMed Central

Mäthger, Lydia M.; Denton, Eric J.; Marshall, N. Justin; Hanlon, Roger T.

2008-01-01

Octopus, squid and cuttlefish are renowned for rapid adaptive coloration that is used for a wide range of communication and camouflage. Structural coloration plays a key role in augmenting the skin patterning that is produced largely by neurally controlled pigmented chromatophore organs. While most iridescence and white scattering is produced by passive reflectance or diffusion, some iridophores in squid are actively controlled via a unique cholinergic, non-synaptic neural system. We review the recent anatomical and experimental evidence regarding the mechanisms of reflection and diffusion of light by the different cell types (iridophores and leucophores) of various cephalopod species. The structures that are responsible for the optical effects of some iridophores and leucophores have recently been shown to be proteins. Optical interactions with the overlying pigmented chromatophores are complex, and the recent measurements are presented and synthesized. Polarized light reflected from iridophores can be passed through the chromatophores, thus enabling the use of a discrete communication channel, because cephalopods are especially sensitive to polarized light. We illustrate how structural coloration contributes to the overall appearance of the cephalopods during intra- and interspecific behavioural interactions including camouflage. PMID:19091688

Loss Aversion Reflects Information Accumulation, Not Bias: A Drift-Diffusion Model Study.

PubMed

Clay, Summer N; Clithero, John A; Harris, Alison M; Reed, Catherine L

2017-01-01

Defined as increased sensitivity to losses, loss aversion is often conceptualized as a cognitive bias. However, findings that loss aversion has an attentional or emotional regulation component suggest that it may instead reflect differences in information processing. To distinguish these alternatives, we applied the drift-diffusion model (DDM) to choice and response time (RT) data in a card gambling task with unknown risk distributions. Loss aversion was measured separately for each participant. Dividing the participants into terciles based on loss aversion estimates, we found that the most loss-averse group showed a significantly lower drift rate than the other two groups, indicating overall slower uptake of information. In contrast, neither the starting bias nor the threshold separation (barrier) varied by group, suggesting that decision thresholds are not affected by loss aversion. These results shed new light on the cognitive mechanisms underlying loss aversion, consistent with an account based on information accumulation.

Loss Aversion Reflects Information Accumulation, Not Bias: A Drift-Diffusion Model Study

PubMed Central

Clay, Summer N.; Clithero, John A.; Harris, Alison M.; Reed, Catherine L.

2017-01-01

Defined as increased sensitivity to losses, loss aversion is often conceptualized as a cognitive bias. However, findings that loss aversion has an attentional or emotional regulation component suggest that it may instead reflect differences in information processing. To distinguish these alternatives, we applied the drift-diffusion model (DDM) to choice and response time (RT) data in a card gambling task with unknown risk distributions. Loss aversion was measured separately for each participant. Dividing the participants into terciles based on loss aversion estimates, we found that the most loss-averse group showed a significantly lower drift rate than the other two groups, indicating overall slower uptake of information. In contrast, neither the starting bias nor the threshold separation (barrier) varied by group, suggesting that decision thresholds are not affected by loss aversion. These results shed new light on the cognitive mechanisms underlying loss aversion, consistent with an account based on information accumulation. PMID:29066987

Handheld dual fluorescence and reflection spectroscopy system for monitoring topical low dose ALA-PDT of actinic keratoses (AK)

NASA Astrophysics Data System (ADS)

Charamisinau, Ivan; Keymel, Kenneth; Potter, William; Oseroff, Allan R.

2006-02-01

Photodynamic therapy is an effective, minimally invasive skin cancer treatment modality with few side effects. Improved therapeutic selectivity and efficacy is expected if treatment is optimized individually for each patient based on detailed measurements prior and during the treatment. The handheld system presented allows measuring optical properties of the skin, the rate of photosensitizer photobleaching during the ALA PDT and oxygen saturation in the tissue. The photobleaching rate is monitored using fluorescence spectroscopy, where protoporphyrin IX in tissue is exited by 410 nm (blue) or 532 nm (green) laser light, and fluorescence in the 580-800 nm range is monitored. The photobleaching rate is calculated by correlating the measured spectrum with known protoporphyrin IX, photoproduct and nonspecific tissue autofluorescence spectra using correlation analysis. Double-wavelength excitation allows a rough estimation of the depth of the fluorescence source due to the significant difference in penetration depth for blue and green light. Blood concentration and oxygenation in the tissue are found from the white light reflectance spectrum in the 460-800 nm range. Known spectra for the oxy- and deoxyhemoglobin, melanin, and tissue baseline absorption and tissue scattering are substituted in nonlinear equations to find the penetration depth and diffuse reflectance coefficient. The nonlinear equation for the diffuse reflectance coefficient is solved for blood and melanin concentrations and blood oxygenation values that provide the best fit to the measured spectrum. The optical properties of the tissue obtained from the reflectance spectroscopy are used to correct the fluorescence data. A noncontact probe with 5 fibers (3 excitation and 2 detection) focused to the same 5 mm diameter spot: 2 excitation lasers, a white light lamp and a two-channel spectrometer are used. A LabView program with custom nonlinear equation solvers written in C++ automatically performs the measurements and calculations, and writes data to a database. The system is currently used in a clinical trial to find the relationship between skin pigmentation, oxygen saturation in blood, photobleaching rate and optimal fluence rate for skin cancer treatment of actinic keratoses.

Comparison of the Radiative Two-Flux and Diffusion Approximations

NASA Technical Reports Server (NTRS)

Spuckler, Charles M.

2006-01-01

Approximate solutions are sometimes used to determine the heat transfer and temperatures in a semitransparent material in which conduction and thermal radiation are acting. A comparison of the Milne-Eddington two-flux approximation and the diffusion approximation for combined conduction and radiation heat transfer in a ceramic material was preformed to determine the accuracy of the diffusion solution. A plane gray semitransparent layer without a substrate and a non-gray semitransparent plane layer on an opaque substrate were considered. For the plane gray layer the material is semitransparent for all wavelengths and the scattering and absorption coefficients do not vary with wavelength. For the non-gray plane layer the material is semitransparent with constant absorption and scattering coefficients up to a specified wavelength. At higher wavelengths the non-gray plane layer is assumed to be opaque. The layers are heated on one side and cooled on the other by diffuse radiation and convection. The scattering and absorption coefficients were varied. The error in the diffusion approximation compared to the Milne-Eddington two flux approximation was obtained as a function of scattering coefficient and absorption coefficient. The percent difference in interface temperatures and heat flux through the layer obtained using the Milne-Eddington two-flux and diffusion approximations are presented as a function of scattering coefficient and absorption coefficient. The largest errors occur for high scattering and low absorption except for the back surface temperature of the plane gray layer where the error is also larger at low scattering and low absorption. It is shown that the accuracy of the diffusion approximation can be improved for some scattering and absorption conditions if a reflectance obtained from a Kubelka-Munk type two flux theory is used instead of a reflection obtained from the Fresnel equation. The Kubelka-Munk reflectance accounts for surface reflection and radiation scattered back by internal scattering sites while the Fresnel reflection only accounts for surface reflections.

In vivo burn diagnosis by camera-phone diffuse reflectance laser speckle detection.

PubMed

Ragol, S; Remer, I; Shoham, Y; Hazan, S; Willenz, U; Sinelnikov, I; Dronov, V; Rosenberg, L; Bilenca, A

2016-01-01

Burn diagnosis using laser speckle light typically employs widefield illumination of the burn region to produce two-dimensional speckle patterns from light backscattered from the entire irradiated tissue volume. Analysis of speckle contrast in these time-integrated patterns can then provide information on burn severity. Here, by contrast, we use point illumination to generate diffuse reflectance laser speckle patterns of the burn. By examining spatiotemporal fluctuations in these time-integrated patterns along the radial direction from the incident point beam, we show the ability to distinguish partial-thickness burns in a porcine model in vivo within the first 24 hours post-burn. Furthermore, our findings suggest that time-integrated diffuse reflectance laser speckle can be useful for monitoring burn healing over time post-burn. Unlike conventional diffuse reflectance laser speckle detection systems that utilize scientific or industrial-grade cameras, our system is designed with a camera-phone, demonstrating the potential for burn diagnosis with a simple imager.

In vivo burn diagnosis by camera-phone diffuse reflectance laser speckle detection

PubMed Central

Ragol, S.; Remer, I.; Shoham, Y.; Hazan, S.; Willenz, U.; Sinelnikov, I.; Dronov, V.; Rosenberg, L.; Bilenca, A.

2015-01-01

Burn diagnosis using laser speckle light typically employs widefield illumination of the burn region to produce two-dimensional speckle patterns from light backscattered from the entire irradiated tissue volume. Analysis of speckle contrast in these time-integrated patterns can then provide information on burn severity. Here, by contrast, we use point illumination to generate diffuse reflectance laser speckle patterns of the burn. By examining spatiotemporal fluctuations in these time-integrated patterns along the radial direction from the incident point beam, we show the ability to distinguish partial-thickness burns in a porcine model in vivo within the first 24 hours post-burn. Furthermore, our findings suggest that time-integrated diffuse reflectance laser speckle can be useful for monitoring burn healing over time post-burn. Unlike conventional diffuse reflectance laser speckle detection systems that utilize scientific or industrial-grade cameras, our system is designed with a camera-phone, demonstrating the potential for burn diagnosis with a simple imager. PMID:26819831

Long-term calibration monitoring of Spectralon diffusers BRDF in the air-ultraviolet.

PubMed

Georgiev, Georgi T; Butler, James J

2007-11-10

Long-term calibration monitoring of the bidirectional reflectance distribution function (BRDF) of Spectralon diffusers in the air-ultraviolet is presented. Four Spectralon diffusers were monitored in this study. Three of the diffusers, designated as H1, H2, and H3, were used in the prelaunch radiance calibration of the Solar Backscatter Ultraviolet/2 (SBUV/2) satellite instruments on National Oceanic and Atmospheric Administration (NOAA) 14 and 16. A fourth diffuser, designated as the 400 diffuser, was used in the prelaunch calibration of the Ozone Mapping and Profiler Suite (OMPS) instrument scheduled for initial flight in 2009 on the National Polar Orbiting Environmental Satellite System Preparatory Project. The BRDF data of this study were obtained between 1994 and 2005 using the scatterometer located in the National Aeronautics and Space Administration Goddard Space Flight Center Diffuser Calibration Laboratory. The diffusers were measured at 13 wavelengths between 230 and 425 nm at the incident and scatter angles used in the prelaunch calibrations of SBUV/2 and OMPS. Spectral features in the BRDF of Spectralon are also discussed. The comparison shows how the air-ultraviolet BRDF of these Spectralon samples changed over time under clean room deployment conditions.

Measuring Moisture Levels in Graphite Epoxy Composite Sandwich Structures

NASA Technical Reports Server (NTRS)

Nurge, Mark; Youngquist, Robert; Starr, Stanley

2011-01-01

Graphite epoxy composite (GEC) materials are used in the construction of rocket fairings, nose cones, interstage adapters, and heat shields due to their high strength and light weight. However, they absorb moisture depending on the environmental conditions they are exposed to prior to launch. Too much moisture absorption can become a problem when temperature and pressure changes experienced during launch cause the water to vaporize. The rapid state change of the water can result in structural failure of the material. In addition, heat and moisture combine to weaken GEC structures. Diffusion models that predict the total accumulated moisture content based on the environmental conditions are one accepted method of determining if the material strength has been reduced to an unacceptable level. However, there currently doesn t exist any field measurement technique to estimate the actual moisture content of a composite structure. A multi-layer diffusion model was constructed with Mathematica to predict moisture absorption and desorption from the GEC sandwich structure. This model is used in conjunction with relative humidity/temperature sensors both on the inside and outside of the material to determine the moisture levels in the structure. Because the core materials have much higher diffusivity than the face sheets, a single relative humidity measurement will accurately reflect the moisture levels in the core. When combined with an external relative humidity measurement, the model can be used to determine the moisture levels in the face sheets. Since diffusion is temperaturedependent, the temperature measurements are used to determine the diffusivity of the face sheets for the model computations.

An in situ method for real-time monitoring of soil gas diffusivity

NASA Astrophysics Data System (ADS)

Laemmel, Thomas; Maier, Martin; Schack-Kirchner, Helmer; Lang, Friederike

2016-04-01

Soil aeration is an important factor for the biogeochemistry of soils. Generally, gas exchange between soil and atmosphere is assumed to be governed by molecular diffusion and by this way fluxes can be calculated using by Fick's Law. The soil gas diffusion coefficient DS represents the proportional factor between the gas flux and the gas concentration gradient in the soil and reflects the ability of the soil to "transport passively" gas through the soil. One common way to determine DS is taking core samples in the field and measuring DS in the lab. Unfortunately this method is destructive and laborious and it can only reflect a small fraction of the whole soil. As a consequence, uncertainty about the resulting effective diffusivity on the profile scale, i.e. the real aeration status remains. We developed a method to measure and monitor DS in situ. The set-up consists of a custom made gas sampling device, the continuous injection of an inert tracer gas and inverse gas transport modelling in the soil. The gas sampling device has seven sampling depths (from 0 to -43 cm of depth) and can be easily installed into vertical holes drilled by an auger, which allows for fast installation of the system. Helium (He) as inert tracer gas was injected continuously at the lower end of the device. The resulting steady state distribution of He was used to deduce the DS depth distribution of the soil. For Finite Element Modeling of the gas-sampling-device/soil system the program COMSOL was used. We tested our new method both in the lab and in a field study and compared the results with a reference lab method using soil cores. DS profiles obtained by our in-situ method were consistent with DS profiles determined based on soil core analyses. Soil gas profiles could be measured with a temporal resolution of 30 minutes. During the field study, there was an important rain event and we could monitor the decrease in soil gas diffusivity in the top soil due to water infiltration. The effect of soil water infiltration deeper into the soil on soil gas diffusivity could be observed during the following hours. Our new DS determination device can be quickly and easily installed and allows for monitoring continuously soil gas transport over a long time. It allows following modifications of soil gas diffusivity due to rain events. In addition it enables the analysis of non-diffusive soil gas transport processes.

Development and application of variable angle internal reflection Raman spectroscopy for vibrationally specific depth-profiling of polymer thin films

NASA Astrophysics Data System (ADS)

Fontaine, Norman Henry

1997-10-01

Techniques which can be used to obtain depth-resolved information on the thermodynamics at polymer-polymer and polymer-wall interfaces, and of small molecule diffusion in polymers, are of particular interest to industry. Optical methods which are sensitive to molecular vibrations (such as internal reflection Raman spectroscopy) are advantageous because they can non- destructively probe molecular content, orientation, and polarity of the local environment in a sample. However, while optical internal reflection depth-profiling methods have been reported, they have never progressed beyond the demonstration stage. In this work, the theory and methodology of internal reflection spectroscopy are developed and optimized into a rigorous field-controlled spectroscopic technique. A novel asymmetric internal reflection element (IRE) is introduced which traps back-reflections, allowing precise evanescent and standing wave probe-field control in the sample for all angles of incidence. It is demonstrated that a Gaussian laser beam will best approximate an infinite homogeneous plane wave when the IRE/sample interface lies in the paraxial-Fraunhofer region (far- field) of the beam path. Calibration methods are presented, sources of systematic errors are identified, and the angular resolution limit (ARL) is introduced as a measure of the field control developed in a sample by any internal reflection method. A general model of Raman scattering and photon detection from multi-layer thin films is developed. A new and generalized operator based transfer matrix method is developed and applied to electromagnetic field and diffusion computations in multi-layer systems. Total internal reflection spectroscopy is extended to include sub-critical angles of incidence, where resonant field enhancements generate large and selective amplification of the probe-field intensity within the layers of the sample. Fitting these resonances to the model spectral intensities allows unique determination of the location of buried interfaces in micron-sized polymer multi-layers with nanometer scale precision and the refractive indices of the layers with precision of /Delta n/approx/pm 0.0001. The Raman active molecular content of each optically distinct layer of the film is determinable simultaneously with the optical properties. Resonant mode VAIRRS studies of poly(methyl methacrylate) films spun-cast from toluene and then dried under ambient conditions have shown evidence for toluene diffusion concurrent with a rotationally hindered relaxation of oriented ester side groups about the polymer backbone. Low temperature annealing (≈87oC) has shown evidence that this hindered rotational relaxation may be reversible. VAIRRS study of a polystyrene/poly(methyl methacrylate) bi-layer has detected evidence for toluene diffusion across the buried polymer-polymer interface.

Diffuse Scattering from Lead-Containing Ferroelectric Perovskite Oxides

DOE PAGES

Goossens, D. J.

2013-01-01

Ferroelectric materials rely on some type of non-centrosymmetric displacement correlations to give rise to a macroscopic polarisation. These displacements can show short-range order (SRO) that is reflective of the local chemistry, and so studying it reveals important information about how the structure gives rise to the technologically useful properties. A key means of exploring this SRO is diffuse scattering. Conventional structural studies use Bragg peak intensitiesto determine the average structure. In a single crystal diffuse scattering (SCDS) experiment, the coherent scattered intensity is measured at non-integer Miller indices, and can be used to examine the population of local configurations. Thismore » is because the diffuse scattering is sensitive to two-body averages, whereas the Bragg intensity gives single-body averages. This review outlines key results of SCDS studies on several materials and explores the similarities and differences in their diffuse scattering. Random strains are considered, as are models based on a phonon-like picture or a more local-chemistry oriented picture. Limitations of the technique are discussed.« less

Transcranial diffuse optical monitoring of microvascular cerebral hemodynamics after thrombolysis in ischemic stroke

NASA Astrophysics Data System (ADS)

Zirak, Peyman; Delgado-Mederos, Raquel; Dinia, Lavinia; Carrera, David; Martí-Fàbregas, Joan; Durduran, Turgut

2014-01-01

The ultimate goal of therapeutic strategies for ischemic stroke is to reestablish the blood flow to the ischemic region of the brain. However, currently, the local cerebral hemodynamics (microvascular) is almost entirely inaccessible for stroke clinicians at the patient bed-side, and the recanalization of the major cerebral arteries (macrovascular) is the only available measure to evaluate the therapy, which does not always reflect the local conditions. Here we report the case of an ischemic stroke patient whose microvascular cerebral blood flow and oxygenation were monitored by a compact hybrid diffuse optical monitor during thrombolytic therapy. This monitor combined diffuse correlation spectroscopy and near-infrared spectroscopy. The reperfusion assessed by hybrid diffuse optics temporally correlated with the recanalization of the middle cerebral artery (assessed by transcranial-Doppler) and was in agreement with the patient outcome. This study suggests that upon further investigation, diffuse optics might have a potential for bed-side acute stroke monitoring and therapy guidance by providing hemodynamics information at the microvascular level.

Transcranial diffuse optical monitoring of microvascular cerebral hemodynamics after thrombolysis in ischemic stroke.

PubMed

Zirak, Peyman; Delgado-Mederos, Raquel; Dinia, Lavinia; Carrera, David; Martí-Fàbregas, Joan; Durduran, Turgut

2014-01-01

The ultimate goal of therapeutic strategies for ischemic stroke is to reestablish the blood flow to the ischemic region of the brain. However, currently, the local cerebral hemodynamics (microvascular) is almost entirely inaccessible for stroke clinicians at the patient bed-side, and the recanalization of the major cerebral arteries (macrovascular) is the only available measure to evaluate the therapy, which does not always reflect the local conditions. Here we report the case of an ischemic stroke patient whose microvascular cerebral blood flow and oxygenation were monitored by a compact hybrid diffuse optical monitor during thrombolytic therapy. This monitor combined diffuse correlation spectroscopy and near-infrared spectroscopy. The reperfusion assessed by hybrid diffuse optics temporally correlated with the recanalization of the middle cerebral artery (assessed by transcranial-Doppler) and was in agreement with the patient outcome. This study suggests that upon further investigation, diffuse optics might have a potential for bed-side acute stroke monitoring and therapy guidance by providing hemodynamics information at the microvascular level.

NMR investigation of the short-chain ionic surfactant-water systems.

PubMed

Popova, M V; Tchernyshev, Y S; Michel, D

2004-02-03

The structure and dynamics of surfactant molecules [CH3(CH2)7COOK] in heavy water solutions were investigated by 1H and 2H NMR. A double-exponential attenuation of the spin-echo amplitude in a Carr-Purcell-Meiboom-Gill experiment was found. We expect correspondence to both bounded and monomeric states. At high concentrations in the NMR self-diffusion measurements also a double-exponential decay of the spin-echo signal versus the square of the dc magnetic gradient was observed. The slow component of the diffusion process is caused by micellar aggregates, while the fast component is the result of the self-diffusion of the monomers through the micelles. The self-diffusion studies indicate that the form of micelles changes with increasing total surfactant concentration. The critical temperature range for self-association is reflected in the 1H transverse relaxation.

Automated test-site radiometer for vicarious calibration

NASA Astrophysics Data System (ADS)

Li, Xin; Yin, Ya-peng; Liu, En-chao; Zhang, Yan-na; Xun, Li-na; Wei, Wei; Zhang, Zhi-peng; Qiu, Gang-gang; Zhang, Quan; Zheng, Xiao-bing

2014-11-01

In order to realize unmanned vicarious calibration, Automated Test-site Radiometer (ATR) was developed for surface reflectance measurements. ATR samples the spectrum from 400nm-1600 nm with 8 interference filters coupled with silicon and InGaAs detectors. The field of view each channel is 10 ° with parallel optical axis. One SWIR channel lies in the center and the other seven VNIR channels are on the circle of 4.8cm diameters which guarantee each channel to view nearly the same section of ground. The optical head as a whole is temperature controlled utilizing a TE cooler for greater stability and lower noise. ATR is powered by a solar panel and transmit its data through a BDS (China's BeiDou Navigation Satellite System) terminator for long-term measurements without personnel in site. ATR deployed in Dunhuang test site with ground field about 30-cm-diameter area for multi-spectral reflectance measurements. Other instruments at the site include a Cimel sunphotometer and a diffuser-to-globe irradiance meter for atmosphere observations. The methodology for band-averaged reflectance retrieval and hyperspectral reflectance fitting process are described. Then the hyperspectral reflectance and atmospheric parameters are put into 6s code to predict TOA radiance which compare with MODIS radiance.

Simultaneous measurement of X-ray specular reflection and off-specular diffuse scattering from liquid surfaces using a two-dimensional pixel array detector: the liquid-interface reflectometer of BL37XU at SPring-8.

PubMed

Yano, Yohko F; Uruga, Tomoya; Tanida, Hajime; Toyokawa, Hidenori; Terada, Yasuko; Yamada, Hironari

2010-07-01

An X-ray reflectometer for simultaneous measurement of specular and off-specular reflection of liquid surfaces is described. The reflectometer, equipped with a two-dimensional single X-ray photon-counting pixel array detector obtained the full range of X-ray specular and off-specular reflections in an extremely short time (1 s). Both the specular and off-specular reflection of water exhibited good agreement with the predicted capillary-wave theory within the appropriate instrumental resolution. The approach is also demonstrated on an aqueous solution of 1-dodecyl-3-methylimidazolium chloride. The monolayer in which the dodecyl chain faces upwards and the Cl(-) anion locates next to the imidazolium ring formed on the water surface was found to be laterally homogeneous. The use of a pixel array detector will be particularly powerful for in situ measurements to investigate both out-of-plane and in-plane structures simultaneously, not only for liquid surfaces but also for other thin films.

SeaWiFS long-term solar diffuser reflectance trend analysis

NASA Astrophysics Data System (ADS)

Eplee, Robert E., Jr.; Patt, Frederick S.; Barnes, Robert A.; McClain, Charles R.

2006-08-01

The NASA Ocean Biology Processing Group's Calibration and Validation (Cal/Val) Team implemented daily solar calibrations of SeaWiFS to look for step-function changes in the instrument response and has used these calibrations to supplement the monthly lunar calibrations in monitoring the radiometric stability of SeaWiFS during its first year of on-orbit operations. The Team has undertaken an analysis of the mission-long solar calibration time series, with the lunar-derived radiometric corrections over time applied, to assess the long-term degradation of the solar diffuser reflectance over nine years on orbit. The SeaWiFS diffuser is an aluminum plate coated with YB71 paint. The bidirectional reflectance distribution function of the diffuser was not fully characterized before launch, so the Cal/Val Team has implemented a regression of the solar incidence angles and the drift in the node of the satellite's orbit against the diffuser time series to correct for solar incidence angle effects. An exponential function with a time constant of 200 days yields the best fit to the diffuser time series. The decrease in diffuser reflectance over the mission is wavelength-dependent, ranging from 9% in the blue (412 nm) to 5% in the red and near infrared (670-865 nm). The degradation of diffuser reflctance is similar to that observed for SeaWiFS radiometric response itself from lunar calibration time series for bands 1-5 (412-555 nm), though the magnitude of the change is four times larger for the diffuser. Evidently, the same optical degradation process has affected both the telescope optics and the solar diffuser in the blue and green. The Cal/Val Team has developed a methodology for computing the signal-to-noise ratio (SNR) for SeaWiFS on orbit from the diffuser time series. The on-orbit change in the SNR for each band over the nine-year mission is less than 7%. The on-orbit performance of the SeaWiFS solar diffuser should offer insight into the long-term on-orbit performance of solar diffusers on other instruments, such as MODIS, VIIRS, and ABI.

DESIGN NOTE: New apparatus for haze measurement for transparent media

NASA Astrophysics Data System (ADS)

Yu, H. L.; Hsiao, C. C.; Liu, W. C.

2006-08-01

Precise measurement of luminous transmittance and haze of transparent media is increasingly important to the LCD industry. Currently there are at least three documentary standards for measuring transmission haze. Unfortunately, none of those standard methods by itself can obtain the precise values for the diffuse transmittance (DT), total transmittance (TT) and haze. This note presents a new apparatus capable of precisely measuring all three variables simultaneously. Compared with current structures, the proposed design contains one more compensatory port. For optimal design, the light trap absorbs the beam completely, light scattered by the instrument is zero and the interior surface of the integrating sphere, baffle, as well as the reflectance standard, are of equal characteristic. The accurate values of the TT, DT and haze can be obtained using the new apparatus. Even if the design is not optimal, the measurement errors of the new apparatus are smaller than those of other methods especially for high sphere reflectance. Therefore, the sphere can be made of a high reflectance material for the new apparatus to increase the signal-to-noise ratio.

Diffuse characteristics study of laser target board using Monte Carlo simulation

NASA Astrophysics Data System (ADS)

Yang, Pengling; Wu, Yong; Wang, Zhenbao; Tao, Mengmeng; Wu, Junjie; Wang, Ping; Yan, Yan; Zhang, Lei; Feng, Gang; Zhu, Jinghui; Feng, Guobin

2013-05-01

In this paper, Torrance-Sparrow and Oren-Nayar model is adopt to study diffuse characteristics of laser target board. The model which based on geometric optics, assumes that rough surfaces are made up of a series of symmetric V-groove cavities with different slopes at microscopic level. The distribution of the slopes of the V-grooves are modeled as beckman distribution function, and every microfacet of the V-groove cavity is assumed to behave like a perfect mirror, which means the reflected ray follows Fresnel law at the microfacet. The masking and shadowing effects of rough surface are also taken into account through geometric attenuation factor. Monte Carlo method is used to simulate the diffuse reflectance distribution of the laser target board with different materials and processing technology, and all the calculated results are verified by experiment. It is shown that the profile of bidirectional reflectance distribution curve is lobe-shaped with the maximum lies along the mirror reflection direction. The width of the profile is narrower for a lower roughness value, and broader for a higher roughness value. The refractive index of target material will also influence the intensity and distribution of diffuse reflectance of laser target surface.

Online high-speed NIR diffuse-reflectance imaging spectroscopy in food quality monitoring

NASA Astrophysics Data System (ADS)

Driver, Richard D.; Didona, Kevin

2009-05-01

The use of hyperspectral technology in the NIR for food quality monitoring is discussed. An example of the use of hyperspectral diffuse reflectance scanning and post-processing with a chemometric model shows discrimination between four pharmaceutical samples comprising Aspirin, Acetaminophen, Vitamin C and Vitamin D.

The Improved Dual-view Field Goniometer System FIGOS

PubMed Central

Schopfer, Jürg; Dangel, Stefan; Kneubühler, Mathias; Itten, Klaus I.

2008-01-01

In spectrodirectional Remote Sensing (RS) the Earth's surface reflectance characteristics are studied by means of their angular dimensions. Almost all natural surfaces exhibit an individual anisotropic reflectance behaviour due to the contrast between the optical properties of surface elements and background and the geometric surface properties of the observed scene. The underlying concept, which describes the reflectance characteristic of a specific surface area, is called the bidirectional reflectance distribution function (BRDF). BRDF knowledge is essential for both correction of directional effects in RS data and quantitative retrieval of surface parameters. Ground-based spectrodirectional measurements are usually performed with goniometer systems. An accurate retrieval of the bidirectional reflectance factors (BRF) from field goniometer measurements requires hyperspectral knowledge of the angular distribution of the reflected and the incident radiation. However, prior to the study at hand, no operational goniometer system was able to fulfill this requirement. This study presents the first dual-view field goniometer system, which is able to simultaneously collect both the reflected and the incident radiation at high angular and spectral resolution and, thus, providing the necessary spectrodirectional datasets to accurately retrieve the surface specific BRF. Furthermore, the angular distribution of the incoming diffuse radiation is characterized for various atmospheric conditions and the BRF retrieval is performed for an artificial target and compared to laboratory spectrodirectional measurement results obtained with the same goniometer system. Suggestions for further improving goniometer systems are given and the need for intercalibration of various goniometers as well as for standardizing spectrodirectional measurements is expressed. PMID:27873805

The Improved Dual-view Field Goniometer System FIGOS.

PubMed

Schopfer, Jürg; Dangel, Stefan; Kneubühler, Mathias; Itten, Klaus I

2008-08-28

In spectrodirectional Remote Sensing (RS) the Earth's surface reflectance characteristics are studied by means of their angular dimensions. Almost all natural surfaces exhibit an individual anisotropic reflectance behaviour due to the contrast between the optical properties of surface elements and background and the geometric surface properties of the observed scene. The underlying concept, which describes the reflectance characteristic of a specific surface area, is called the bidirectional reflectance distribution function (BRDF). BRDF knowledge is essential for both correction of directional effects in RS data and quantitative retrieval of surface parameters. Ground-based spectrodirectional measurements are usually performed with goniometer systems. An accurate retrieval of the bidirectional reflectance factors (BRF) from field goniometer measurements requires hyperspectral knowledge of the angular distribution of the reflected and the incident radiation. However, prior to the study at hand, no operational goniometer system was able to fulfill this requirement. This study presents the first dual-view field goniometer system, which is able to simultaneously collect both the reflected and the incident radiation at high angular and spectral resolution and, thus, providing the necessary spectrodirectional datasets to accurately retrieve the surface specific BRF. Furthermore, the angular distribution of the incoming diffuse radiation is characterized for various atmospheric conditions and the BRF retrieval is performed for an artificial target and compared to laboratory spectrodirectional measurement results obtained with the same goniometer system. Suggestions for further improving goniometer systems are given and the need for intercalibration of various goniometers as well as for standardizing spectrodirectional measurements is expressed.

How attention influences perceptual decision making: Single-trial EEG correlates of drift-diffusion model parameters

PubMed Central

Nunez, Michael D.; Vandekerckhove, Joachim; Srinivasan, Ramesh

2016-01-01

Perceptual decision making can be accounted for by drift-diffusion models, a class of decision-making models that assume a stochastic accumulation of evidence on each trial. Fitting response time and accuracy to a drift-diffusion model produces evidence accumulation rate and non-decision time parameter estimates that reflect cognitive processes. Our goal is to elucidate the effect of attention on visual decision making. In this study, we show that measures of attention obtained from simultaneous EEG recordings can explain per-trial evidence accumulation rates and perceptual preprocessing times during a visual decision making task. Models assuming linear relationships between diffusion model parameters and EEG measures as external inputs were fit in a single step in a hierarchical Bayesian framework. The EEG measures were features of the evoked potential (EP) to the onset of a masking noise and the onset of a task-relevant signal stimulus. Single-trial evoked EEG responses, P200s to the onsets of visual noise and N200s to the onsets of visual signal, explain single-trial evidence accumulation and preprocessing times. Within-trial evidence accumulation variance was not found to be influenced by attention to the signal or noise. Single-trial measures of attention lead to better out-of-sample predictions of accuracy and correct reaction time distributions for individual subjects. PMID:28435173

How attention influences perceptual decision making: Single-trial EEG correlates of drift-diffusion model parameters.

PubMed

Nunez, Michael D; Vandekerckhove, Joachim; Srinivasan, Ramesh

2017-02-01

Perceptual decision making can be accounted for by drift-diffusion models, a class of decision-making models that assume a stochastic accumulation of evidence on each trial. Fitting response time and accuracy to a drift-diffusion model produces evidence accumulation rate and non-decision time parameter estimates that reflect cognitive processes. Our goal is to elucidate the effect of attention on visual decision making. In this study, we show that measures of attention obtained from simultaneous EEG recordings can explain per-trial evidence accumulation rates and perceptual preprocessing times during a visual decision making task. Models assuming linear relationships between diffusion model parameters and EEG measures as external inputs were fit in a single step in a hierarchical Bayesian framework. The EEG measures were features of the evoked potential (EP) to the onset of a masking noise and the onset of a task-relevant signal stimulus. Single-trial evoked EEG responses, P200s to the onsets of visual noise and N200s to the onsets of visual signal, explain single-trial evidence accumulation and preprocessing times. Within-trial evidence accumulation variance was not found to be influenced by attention to the signal or noise. Single-trial measures of attention lead to better out-of-sample predictions of accuracy and correct reaction time distributions for individual subjects.

Dot Projection Photogrammetric Technique for Shape Measurements of Aerospace Test Articles

NASA Technical Reports Server (NTRS)

Jones, Thomas W.; Pappa, Richard S.

2002-01-01

Results from initial laboratory investigations with the dot projection photogrammetric technique are presented for three wind-tunnel test articles with a range of surface scattering and reflection properties. These test articles are a semispan model and a micro air vehicle with a latex wing that are both diffusely reflecting, and a highly polished specularly reflecting model used for high Reynolds number testing. Results using both white light and laser illumination are presented. Some of the advantages and limitations of the dot projection technique are discussed. Although a desirable final outcome of this research effort is the characterization of dynamic behavior, only static laboratory results are presented in this preliminary effort.

Direct measurements of protein-stabilized gold nanoparticle interactions.

PubMed

Eichmann, Shannon L; Bevan, Michael A

2010-09-21

We report integrated video and total internal reflection microscopy measurements of protein stabilized 110 nm Au nanoparticles confined in 280 nm gaps in physiological media. Measured potential energy profiles display quantitative agreement with Brownian dynamic simulations that include hydrodynamic interactions and camera exposure time and noise effects. Our results demonstrate agreement between measured nonspecific van der Waals and adsorbed protein interactions with theoretical potentials. Confined, lateral nanoparticle diffusivity measurements also display excellent agreement with predictions. These findings provide a basis to interrogate specific biomacromolecular interactions in similar experimental configurations and to design future improved measurement methods.

Quantum efficiency investigations of type-II InAs/GaSb midwave infrared superlattice photodetectors

DOE Office of Scientific and Technical Information (OSTI.GOV)

Giard, E., E-mail: edouard.giard@onera.fr; Ribet-Mohamed, I.; Jaeck, J.

2014-07-28

We present in this paper a comparison between different type-II InAs/GaSb superlattice (T2SL) photodiodes and focal plane array (FPA) in the mid-wavelength infrared domain to understand which phenomenon drives the performances of the T2SL structure in terms of quantum efficiency (QE). Our measurements on test photodiodes suggest low minority carrier diffusion length in the “InAs-rich” design, which penalizes carriers' collection in this structure for low bias voltage and front side illumination. This analysis is completed by a comparison of the experimental data with a fully analytic model, which allows to infer a hole diffusion length shorter than 100 nm. In addition,more » measurements on a FPA with backside illumination are finally presented. Results show an average QE in the 3–4.7 μm window equal to 42% for U{sub bias} = −0.1 V, 77 K operating temperature and no anti-reflection coating. These measurements, completed by modulation transfer function and noise measurements, reveal that the InAs-rich design, despite a low hole diffusion length, is promising for high performance infrared imaging applications.« less

Characterization of a time-resolved non-contact scanning diffuse optical imaging system exploiting fast-gated single-photon avalanche diode detection

DOE Office of Scientific and Technical Information (OSTI.GOV)

Di Sieno, Laura, E-mail: laura.disieno@polimi.it; Dalla Mora, Alberto; Contini, Davide

2016-03-15

We present a system for non-contact time-resolved diffuse reflectance imaging, based on small source-detector distance and high dynamic range measurements utilizing a fast-gated single-photon avalanche diode. The system is suitable for imaging of diffusive media without any contact with the sample and with a spatial resolution of about 1 cm at 1 cm depth. In order to objectively assess its performances, we adopted two standardized protocols developed for time-domain brain imagers. The related tests included the recording of the instrument response function of the setup and the responsivity of its detection system. Moreover, by using liquid turbid phantoms with absorbingmore » inclusions, depth-dependent contrast and contrast-to-noise ratio as well as lateral spatial resolution were measured. To illustrate the potentialities of the novel approach, the characteristics of the non-contact system are discussed and compared to those of a fiber-based brain imager.« less

Information loss and reconstruction in diffuse fluorescence tomography

PubMed Central

Bonfert-Taylor, Petra; Leblond, Frederic; Holt, Robert W.; Tichauer, Kenneth; Pogue, Brian W.; Taylor, Edward C.

2012-01-01

This paper is a theoretical exploration of spatial resolution in diffuse fluorescence tomography. It is demonstrated that, given a fixed imaging geometry, one cannot—relative to standard techniques such as Tikhonov regularization and truncated singular value decomposition—improve the spatial resolution of the optical reconstructions via increasing the node density of the mesh considered for modeling light transport. Using techniques from linear algebra, it is shown that, as one increases the number of nodes beyond the number of measurements, information is lost by the forward model. It is demonstrated that this information cannot be recovered using various common reconstruction techniques. Evidence is provided showing that this phenomenon is related to the smoothing properties of the elliptic forward model that is used in the diffusion approximation to light transport in tissue. This argues for reconstruction techniques that are sensitive to boundaries, such as L1-reconstruction and the use of priors, as well as the natural approach of building a measurement geometry that reflects the desired image resolution. PMID:22472763

ADP Compartmentation Analysis Reveals Coupling between Pyruvate Kinase and ATPases in Heart Muscle

PubMed Central

Sepp, Mervi; Vendelin, Marko; Vija, Heiki; Birkedal, Rikke

2010-01-01

Abstract Cardiomyocytes have intracellular diffusion restrictions, which spatially compartmentalize ADP and ATP. However, the models that predict diffusion restrictions have used data sets generated in rat heart permeabilized fibers, where diffusion distances may be heterogeneous. This is avoided by using isolated, permeabilized cardiomyocytes. The aim of this work was to analyze the intracellular diffusion of ATP and ADP in rat permeabilized cardiomyocytes. To do this, we measured respiration rate, ATPase rate, and ADP concentration in the surrounding solution. The data were analyzed using mathematical models that reflect different levels of cell compartmentalization. In agreement with previous studies, we found significant diffusion restriction by the mitochondrial outer membrane and confirmed a functional coupling between mitochondria and a fraction of ATPases in the cell. In addition, our experimental data show that considerable activity of endogenous pyruvate kinase (PK) remains in the cardiomyocytes after permeabilization. A fraction of ATPases were inactive without ATP feedback by this endogenous PK. When analyzing the data, we were able to reproduce the measurements only with the mathematical models that include a tight coupling between the fraction of endogenous PK and ATPases. To our knowledge, this is the first time such a strong coupling of PK to ATPases has been demonstrated in permeabilized cardiomyocytes. PMID:20550890

N-acetyl-aspartate levels correlate with intra-axonal compartment parameters from diffusion MRI.

PubMed

Grossman, Elan J; Kirov, Ivan I; Gonen, Oded; Novikov, Dmitry S; Davitz, Matthew S; Lui, Yvonne W; Grossman, Robert I; Inglese, Matilde; Fieremans, Els

2015-09-01

Diffusion MRI combined with biophysical modeling allows for the description of a white matter (WM) fiber bundle in terms of compartment specific white matter tract integrity (WMTI) metrics, which include intra-axonal diffusivity (Daxon), extra-axonal axial diffusivity (De||), extra-axonal radial diffusivity (De┴), axonal water fraction (AWF), and tortuosity (α) of extra-axonal space. Here we derive these parameters from diffusion kurtosis imaging to examine their relationship to concentrations of global WM N-acetyl-aspartate (NAA), creatine (Cr), choline (Cho) and myo-Inositol (mI), as measured with proton MR spectroscopy ((1)H-MRS), in a cohort of 25 patients with mild traumatic brain injury (MTBI). We found statistically significant (p<0.05) positive correlations between NAA and Daxon, AWF, α, and fractional anisotropy; negative correlations between NAA and De,┴ and the overall radial diffusivity (D┴). These correlations were supported by similar findings in regional analysis of the genu and splenium of the corpus callosum. Furthermore, a positive correlation in global WM was noted between Daxon and Cr, as well as a positive correlation between De|| and Cho, and a positive trend between De|| and mI. The specific correlations between NAA, an endogenous probe of the neuronal intracellular space, and WMTI metrics related to the intra-axonal space, combined with the specific correlations of De|| with mI and Cho, both predominantly present extra-axonally, corroborate the overarching assumption of many advanced modeling approaches that diffusion imaging can disentangle between the intra- and extra-axonal compartments in WM fiber bundles. Our findings are also generally consistent with what is known about the pathophysiology of MTBI, which appears to involve both intra-axonal injury (as reflected by a positive trend between NAA and Daxon) as well as axonal shrinkage, demyelination, degeneration, and/or loss (as reflected by correlations between NAA and De┴, AWF, and α). Copyright © 2015 Elsevier Inc. All rights reserved.

Atmospheric and Science Complexity Effects on Surface Bidirectional Reflectance

NASA Technical Reports Server (NTRS)

Diner, D. J. (Principal Investigator); Martonchik, J. V.; Sythe, W. D.; Hessom, C.

1985-01-01

Among the tools used in passive remote sensing of Earth resources in the visible and near-infrared spectral regions are measurements of spectral signature and bidirectional reflectance functions (BDRFs). Determination of surface properties using these observables is complicated by a number of factors, including: (1) mixing of surface components, such as soil and vegetation, (2) multiple reflections of radiation due to complex geometry, such as in crop canopies, and (3) atmospheric effects. In order to bridge the diversity in these different approaches, there is a need for a fundamental physical understanding of the influence of the various effects and a quantiative measure of their relative importance. In particular, we consider scene complexity effects using the example of reflection by vegetative surfaces. The interaction of sunlight with a crop canopy and interpretation of the spectral and angular dependence of the emergent radiation is basically a multidimensional radiative transfer problem. The complex canopy geometry, underlying soil cover, and presence of diffuse as well as collimated illumination will modify the reflectance characteristics of the canopy relative to those of the individual elements.

Measurement of drug and macromolecule diffusion across atherosclerotic rabbit aorta ex vivo by attenuated total reflection-Fourier transform infrared imaging

NASA Astrophysics Data System (ADS)

Palombo, Francesca; Danoux, Charlène B.; Weinberg, Peter D.; Kazarian, Sergei G.

2009-07-01

Diffusion of two model drugs-benzyl nicotinate and ibuprofen-and the plasma macromolecule albumin across atherosclerotic rabbit aorta was studied ex vivo by attenuated total reflection-Fourier transform infrared (ATR-FTIR) imaging. Solutions of these molecules were applied to the endothelial surface of histological sections of the aortic wall that were sandwiched between two impermeable surfaces. An array of spectra, each corresponding to a specific location in the section, was obtained at various times during solute diffusion into the wall and revealed the distribution of the solutes within the tissue. Benzyl nicotinate in Ringer's solution showed higher affinity for atherosclerotic plaque than for apparently healthy tissue. Transmural concentration profiles for albumin demonstrated its permeation across the section and were consistent with a relatively low distribution volume for the macromolecule in the middle of the wall. The ability of albumin to act as a drug carrier for ibuprofen, otherwise undetected within the tissue, was demonstrated by multivariate subtraction image analysis. In conclusion, ATR-FTIR imaging can be used to study transport processes in tissue samples with high spatial and temporal resolution and without the need to label the solutes under study.

Calculation of Transfer Functions of Multilayer Biotissues in the Problems of Correction of Their Fluorescence Spectra

NASA Astrophysics Data System (ADS)

Lysenko, S. A.

2018-01-01

A method for rapid calculation of a flux of stimulated fluorescence of a multilayer optically dense medium with inhomogeneous distribution of the fluorophore has been developed. The light field in the medium at the excitation wavelength of fluorescence is represented by a superposition of incident collimated, incident diffuse, and reflected diffuse fluxes. A two-stream approximation is used to describe the light field in the medium at the wavelength of emission of the fluorescence. Fluxes in adjacent elementary layers of the medium and on its surface are connected by simple matrix operators that are obtained using a combination of engineering approaches of radiation-transfer theory and single-scattering approximation. The calculations of fluorescence fluxes of a four-layer biotissue that are excited and recorded at 400-800 nm are compared with their Monte Carlo simulation with a discrepancy of 1%. The effect of the propagation medium on the fluorescence spectra of 5-ALA-induced protoporphyrin IX that are recorded from human skin was studied, and a technique for their correction that is based on measurements and quantitative analysis of the diffuse reflectance spectrum of skin was proposed.

Polaradiometric pyrometer in which the parallel and perpendicular components of radiation reflected from an unpolarized light source are equalized with the thermal radiation emitted from a measured object to determine its true temperature

NASA Technical Reports Server (NTRS)

Abtahi, Ali A. (Inventor)

1995-01-01

A radiation pyrometer for measuring the true temperature of a body is provided by detecting and measuring thermal radiation from the body based on the principle that the effects of angular emission I(sub 1) and reflection I(sub 2) on the polarization states p and s of radiation are complementary such that upon detecting the combined partial polarization state components I(sub p) =I(sub 1p) + I(sub 2p) and I(sub s)=I(sub 1s) + I(sub 2s) and adjusting the intensity of the variable radiation source of the reflected radiation I(sub 2) until the combined partial radiation components I(sub p) and I(sub s) are equal, the effects of emissivity as well as diffusivity of the surface of the body are eliminated, thus obviating the need for any post processing of brightness temperature data.

Infrared reflectometry of skin: Analysis of backscattered light from different skin layers

NASA Astrophysics Data System (ADS)

Pleitez, Miguel A.; Hertzberg, Otto; Bauer, Alexander; Lieblein, Tobias; Glasmacher, Mathias; Tholl, Hans; Mäntele, Werner

2017-09-01

We have recently reported infrared spectroscopy of human skin in vivo using quantum cascade laser excitation and photoacoustic or photothermal detection for non-invasive glucose measurement . Here, we analyze the IR light diffusely reflected from skin layers for spectral contributions of glucose. Excitation of human skin by an external cavity tunable quantum cascade laser in the spectral region from 1000 to 1245 cm- 1, where glucose exhibits a fingerprint absorption, yields reflectance spectra with some contributions from glucose molecules. A simple three-layer model of skin was used to calculate the scattering intensities from the surface and from shallow and deeper layers using the Boltzmann radiation transfer equation. Backscattering of light at wavelengths around 10 μm from the living skin occurs mostly from the Stratum corneum top layers and the shallow layers of the living epidermis. The analysis of the polarization of the backscattered light confirms this calculation. Polarization is essentially unchanged; only a very small fraction (< 3%) is depolarized at 90° with respect to the laser polarization set at 0°. Based on these findings, we propose that the predominant part of the backscattered light is due to specular reflectance and to scattering from layers close to the surface. Diffusely reflected light from deeper layers undergoing one or more scattering processes would appear with significantly altered polarization. We thus conclude that a non-invasive glucose measurement based on backscattering of IR light from skin would have the drawback that only shallow layers containing some glucose at concentrations only weakly related to blood glucose are monitored.

A comparative study of mid-infrared diffuse reflection (DR) and attenuated total reflection (ATR) spectroscopy for the detection of fungal infection on RWA2-corn.

PubMed

Kos, Gregor; Krska, Rudolf; Lohninger, Hans; Griffiths, Peter R

2004-01-01

An investigation into the rapid detection of mycotoxin-producing fungi on corn by two mid-infrared spectroscopic techniques was undertaken. Corn samples from a single genotype (RWA2, blanks, and contaminated with Fusarium graminearum) were ground, sieved and, after appropriate sample preparation, subjected to mid-infrared spectroscopy using two different accessories (diffuse reflection and attenuated total reflection). The measured spectra were evaluated with principal component analysis (PCA) and the blank and contaminated samples were classified by cluster analysis. Reference data for fungal metabolites were obtained with conventional methods. After extraction and clean-up, each sample was analyzed for the toxin deoxynivalenol (DON) by gas chromatography with electron capture detection (GC-ECD) and ergosterol (a parameter for the total fungal biomass) by high-performance liquid chromatography with diode array detection (HPLC-DAD). The concentration ranges for contaminated samples were 880-3600 microg/kg for ergosterol and 300-2600 microg/kg for DON. Classification efficiency was 100% for ATR spectra. DR spectra did not show as obvious a clustering of contaminated and blank samples. Results and trends were also observed in single spectra plots. Quantification using a PLS1 regression algorithm showed good correlation with DON reference data, but a rather high standard error of prediction (SEP) with 600 microg/kg (DR) and 490 microg/kg (ATR), respectively, for ergosterol. Comparing measurement procedures and results showed advantages for the ATR technique, mainly owing to its ease of use and the easier interpretation of results that were better with respect to classification and quantification.

Limitations of the commonly used simplified laterally uniform optical fiber probe-tissue interface in Monte Carlo simulations of diffuse reflectance

PubMed Central

Naglič, Peter; Pernuš, Franjo; Likar, Boštjan; Bürmen, Miran

2015-01-01

Light propagation models often simplify the interface between the optical fiber probe tip and tissue to a laterally uniform boundary with mismatched refractive indices. Such simplification neglects the precise optical properties of the commonly used probe tip materials, e.g. stainless steel or black epoxy. In this paper, we investigate the limitations of the laterally uniform probe-tissue interface in Monte Carlo simulations of diffuse reflectance. In comparison to a realistic probe-tissue interface that accounts for the layout and properties of the probe tip materials, the simplified laterally uniform interface is shown to introduce significant errors into the simulated diffuse reflectance. PMID:26504647

[Application of near-infrared diffuse reflectance spectroscopy to the detection and identification of transgenic corn].

PubMed

Rui, Yu-kui; Luo, Yun-bo; Huang, Kun-lun; Wang, Wei-min; Zhang, Lu-da

2005-10-01

With the rapid development of the GMO, more and more GMO food has been pouring into the market. Much attention has been paid to GMO labeling under the controversy of GMO safety. Transgenic corns and their parents were scanned by continuous wave of near infrared diffuse reflectance spectroscopy range of 12000-4000 cm(-1); the resolution was 4 cm(-1); scanning was carried out for 64 times; BP algorithm was applied for data processing. The GMO food was easily resolved. Near-infrared diffuse reflectance spectroscopy is unpolluted and inexpensive compared with PCR and ELISA, so it is a very promising detection method for GMO food.

Magnetic, Mössbauer and optical spectroscopic properties of the AFe3O(PO4)3 (A = Ca, Sr, Pb) series of powder compounds

NASA Astrophysics Data System (ADS)

El Hafid, Hassan; Velázquez, Matias; El Jazouli, Abdelaziz; Wattiaux, Alain; Carlier, Dany; Decourt, Rodolphe; Couzi, Michel; Goldner, Philippe; Delmas, Claude

2014-10-01

AFe3O(PO4)3 (A = Ca, Sr and Pb) powder compounds were studied by means of X-ray diffraction (XRD), electron-probe microanalysis (EPMA) coupled with wavelength dispersion spectroscopy (WDS), Raman and diffuse reflectance spectroscopies, specific heat and magnetic properties measurements. Magnetization, magnetic susceptibility and specific heat measurements carried out on AFe3O(PO4)3 (A = Sr, Ca and Pb) powders firmly establish a series of three ferromagnetic (FM)-like second order phase transitions spanned over the 32-8 K temperature range. Room temperature Mössbauer spectroscopy and associated DFT calculations confirm the existence of three crystallographically non equivalent Fe3+ sites in the three compounds. Mössbauer spectra recorded as a function of temperature in the PbFe3O(PO4)3 compound also establishes the occurrence of two purely magnetic and reversible phase transitions at 32 and 10 K. Diffuse reflectance measurements reveal two broad absorption bands at 1047 and 837 nm, in both PbFe3O(PO4)3 and SrFe3O(PO4)3 powders, with peak cross sections ∼10-20 cm2 typical of spin-forbidden and forced electric dipole intraconfigurational transitions.

Optical properties of cells with melanin

NASA Astrophysics Data System (ADS)

Rohde, Barukh; Coats, Israel; Krueger, James; Gareau, Dan

2014-02-01

The optical properties of pigmented lesions have been studied using diffuse reflectance spectroscopy in a noninvasive configuration on optically thick samples such as skin in vivo. However, it is difficult to un-mix the effects of absorption and scattering with diffuse reflectance spectroscopy techniques due to the complex anatomical distributions of absorbing and scattering biomolecules. We present a device and technique that enables absorption and scattering measurements of tissue volumes much smaller than the optical mean-free path. Because these measurements are taken on fresh-frozen sections, they are direct measurements of the optical properties of tissue, albeit in a different hydration state than in vivo tissue. Our results on lesions from 20 patients including melanomas and nevi show the absorption spectrum of melanin in melanocytes and basal keratinocytes. Our samples consisted of fresh frozen sections that were unstained. Fitting the spectrum as an exponential decay between 500 and 1100 nm [mua = A*exp(-B*(lambda-C)) + D], we report on the fit parameters of and their variation due to biological heterogeneity as A = 4.20e4 +/- 1.57e5 [1/cm], B = 4.57e-3 +/- 1.62e-3 [1/nm], C = 210 +/- 510 [nm] , D = 613 +/- 534 [1/cm]. The variability in these results is likely due to highly heterogeneous distributions of eumelanin and pheomelanin.

In vivo characterization of colorectal metastases in human liver using diffuse reflectance spectroscopy: toward guidance in oncological procedures

NASA Astrophysics Data System (ADS)

Spliethoff, Jarich W.; de Boer, Lisanne L.; Meier, Mark A. J.; Prevoo, Warner; de Jong, Jeroen; Kuhlmann, Koert; Bydlon, Torre M.; Sterenborg, Henricus J. C. M.; Hendriks, Benno H. W.; Ruers, Theo J. M.

2016-09-01

There is a strong need to develop clinical instruments that can perform rapid tissue assessment at the tip of smart clinical instruments for a variety of oncological applications. This study presents the first in vivo real-time tissue characterization during 24 liver biopsy procedures using diffuse reflectance (DR) spectroscopy at the tip of a core biopsy needle with integrated optical fibers. DR measurements were performed along each needle path, followed by biopsy of the target lesion using the same needle. Interventional imaging was coregistered with the DR spectra. Pathology results were compared with the DR spectroscopy data at the final measurement position. Bile was the primary discriminator between normal liver tissue and tumor tissue. Relative differences in bile content matched with the tissue diagnosis based on histopathological analysis in all 24 clinical cases. Continuous DR measurements during needle insertion in three patients showed that the method can also be applied for biopsy guidance or tumor recognition during surgery. This study provides an important validation step for DR spectroscopy-based tissue characterization in the liver. Given the feasibility of the outlined approach, it is also conceivable to make integrated fiber-optic tools for other clinical procedures that rely on accurate instrument positioning.

Transfer matrix method for four-flux radiative transfer.

PubMed

Slovick, Brian; Flom, Zachary; Zipp, Lucas; Krishnamurthy, Srini

2017-07-20

We develop a transfer matrix method for four-flux radiative transfer, which is ideally suited for studying transport through multiple scattering layers. The model predicts the specular and diffuse reflection and transmission of multilayer composite films, including interface reflections, for diffuse or collimated incidence. For spherical particles in the diffusion approximation, we derive closed-form expressions for the matrix coefficients and show remarkable agreement with numerical Monte Carlo simulations for a range of absorption values and film thicknesses, and for an example multilayer slab.

The quality of geological information derivable from high resolution reflectance spectra - Results for mafic silicates

NASA Technical Reports Server (NTRS)

Cloutis, E. A.; Lambert, J.; Smith, D. G. W.; Gaffey, M. J.

1987-01-01

High-resolution visible and near-infrared diffuse reflectance spectra of mafic silicates can be deconvolved to yield quantitative information concerning mineral mixture properties, and the results can be directly applied to remotely sensed data. Spectral reflectance measurements of laboratory mixtures of olivine, orthophyroxene, and clinopyroxene with known chemistries, phase abundances, and particle size distributions have been utilized to develop correlations between spectral properties and the physicochemical parameters of the samples. A large number of mafic silicate spectra were measured and examined for systematic variations in spectral properties as a function of chemistry, phase abundance, and particle size. Three classes of spectral parameters (ratioed, absolute, and wavelength) were examined for any correlations. Each class is sensitive to particular mafic silicate properties. Spectral deconvolution techniques have been developed for quantifying, with varying degrees of accuracy, the assemblage properties (chemistry, phase abundance, and particle size).

Bidirectional reflectance distribution function based surface modeling of non-Lambertian using intensity data of light detection and ranging.

PubMed

Li, Xiaolu; Liang, Yu; Xu, Lijun

2014-09-01

To provide a credible model for light detection and ranging (LiDAR) target classification, the focus of this study is on the relationship between intensity data of LiDAR and the bidirectional reflectance distribution function (BRDF). An integration method based on the built-in-lab coaxial laser detection system was advanced. A kind of intermediary BRDF model advanced by Schlick was introduced into the integration method, considering diffuse and specular backscattering characteristics of the surface. A group of measurement campaigns were carried out to investigate the influence of the incident angle and detection range on the measured intensity data. Two extracted parameters r and S(λ) are influenced by different surface features, which illustrate the surface features of the distribution and magnitude of reflected energy, respectively. The combination of two parameters can be used to describe the surface characteristics for target classification in a more plausible way.

Diffuse-reflectance fourier-transform mid-infrared spectroscopy as a method of characterizing changes in soil organic matter

USDA-ARS?s Scientific Manuscript database

Diffuse-Reflectance Fourier-Transform Mid-Infrared Spectroscopy (MidIR) can identify the presence of important organic functional groups in soil organic matter (SOM). Soils contain myriad organic and inorganic components that absorb in the MidIR so spectral interpretation needs to be validated in or...

Photopolarimetric properties of leaf and vegetation covers over a wide range of measurement directions

NASA Astrophysics Data System (ADS)

Sun, Zhongqiu; Peng, Zhiyan; Wu, Di; Lv, Yunfeng

2018-02-01

The optical scattering property of the target is the essential signal for passive remote sensing applications. To deepen our understanding of the light reflected from vegetation, we present results of photopolarimetric laboratory measurements from single leaf and two vegetation covers (planophile and erectophile) over a wide range of viewing directions. The bidirectional polarized reflectance factor (BPRF) was used to characterize the polarization property of our samples. We observed positive and negative polarization (-BPRFQ) of all samples in the forward scattering and backward scattering directions, respectively. Based on the comparison of the BPRF among single leaf, planophile vegetation and erectophile vegetation, our measurements demonstrate that the orientation of the leaf is a key factor in describing the amount of polarization in the forward scattering direction. Our measurements also validated certain model results stating that (1) specular reflection generates a portion of polarization in the forward scattering direction and diffuses scattering of polarized light in all hemisphere directions, (2) BPRFU is anti-symmetric in the principal plane from a recent study in which the authors simulated the polarized reflectance of vegetation cover using the vector radiative transfer theory. These photopolarimetric measurement results, which can be completely explained by the theoretical results, are useful in remote sensing applications to vegetation.

Optical spectroscopic studies of animal skin used in modeling of human cutaneous tissue

NASA Astrophysics Data System (ADS)

Drakaki, E.; Makropoulou, M.; Serafetinides, A. A.; Borisova, E.; Avramov, L.; Sianoudis, J. A.

2007-03-01

Optical spectroscopy and in particular laser-induced autofluorescence spectroscopy (LIAFS) and diffuse reflectance spectroscopy (DRS), provide excellent possibilities for real-time, noninvasive diagnosis of different skin tissue pathologies. However, the introduction of optical spectroscopy in routine medical practice demands a statistically important data collection, independent from the laser sources and detectors used. The scientists collect databases either from patients, in vivo, or they study different animal models to obtain objective information for the optical properties of various types of normal and diseased tissue. In the present work, the optical properties (fluorescence and reflectance) of two animal skin models are investigated. The aim of using animal models in optical spectroscopy investigations is to examine the statistics of the light induced effects firstly on animals, before any extrapolation effort to humans. A nitrogen laser (λ=337.1 nm) was used as an excitation source for the autofluorescence measurements, while a tungsten-halogen lamp was used for the reflectance measurements. Samples of chicken and pig skin were measured in vitro and were compared with results obtained from measurements of normal human skin in vivo. The specific features of the measured reflectance and fluorescence spectra are discussed, while the limits of data extrapolation for each skin type are also depicted.

Sweat Rate Prediction Equations for Outdoor Exercise with Transient Solar Radiation

DTIC Science & Technology

2012-01-01

AD] 15 Interchangeable variables gSL W/m2 Global solar load Direct weather station data; pyranometer values 25 Direct measurement from weather station ...Fanger equations 2, 4, 13, Direct or weather station values Rdif W Diffuse irradiance Rref W Reflected irradiance AD m2 Body surface area (BSA) from DuBois...assuming the given weather station uses standard meteorological measuring instru- ments. In the heat flow form expressed by Matthew et al. (25

Coastal Atmosphere and Sea Time Series (CoASTS)

NASA Technical Reports Server (NTRS)

Hooker, Stanford B. (Editor); Firestone, Elaine R. (Editor); Berthon, Jean-Francoise; Zibordi, Giuseppe; Doyle, John P.; Grossi, Stefania; vanderLinde, Dirk; Targa, Cristina; McClain, Charles R. (Technical Monitor)

2002-01-01

In this document, the first three years of a time series of bio-optical marine and atmospheric measurements are presented and analyzed. These measurements were performed from an oceanographic tower in the northern Adriatic Sea within the framework of the Coastal Atmosphere and Sea Time Series (CoASTS) project, an ocean color calibration and validation activity. The data set collected includes spectral measurements of the in-water apparent (diffuse attenuation coefficient, reflectance, Q-factor, etc.) and inherent (absorption and scattering coefficients) optical properties, as well as the concentrations of the main optical components (pigment and suspended matter concentrations). Clear seasonal patterns are exhibited by the marine quantities on which an appreciable short-term variability (on the order of a half day to one day) is superimposed. This short-term variability is well correlated with the changes in salinity at the surface resulting from the southward transport of freshwater coming from the northern rivers. Concentrations of chlorophyll alpha and total suspended matter span more than two orders of magnitude. The bio-optical characteristics of the measurement site pertain to both Case-I (about 64%) and Case-II (about 36%) waters, based on a relationship between the beam attenuation coefficient at 660nm and the chlorophyll alpha concentration. Empirical algorithms relating in-water remote sensing reflectance ratios and optical components or properties of interest (chlorophyll alpha, total suspended matter, and the diffuse attenuation coefficient) are presented.

Hybrid Diffuse Reflectance Spectroscopy: Non-Erythemal in vivo Testing of Sun Protection Factor.

PubMed

Rohr, Mathias; Ernst, Nikolai; Schrader, Andreas

2018-01-01

In order to define a label sun protection factor (SPF) of topically applied sunscreens, in vivo test methods like ISO 24444, FDA guideline, or the Australian standard are used worldwide. The basis of all these methods is provoking an erythemal skin reaction by UV irradiation to find the level of unprotected and protected minimal erythemal doses (MED). In vitro methods replacing the human skin by any kind of non-human material are still not available. Thus, offering the new hybrid diffuse reflectance spectroscopy (HDRS) technique that is able to stay on an in vivo level for SPF testing but meanwhile neglecting the UV-dose-related erythemal skin reaction is a perfect combination to take care of sun protection and any ethical concerns in SPF testing nowadays. HDRS is a combination of in vivo diffuse reflectance spectroscopy (DRS) measurements on the skin and in vitro transmission measurements of a sunscreen on a roughened polymethylmethacrylate plate. By this technique, the in vivo behavior of the investigated sunscreen on the skin is measured as well as the UVB absorption, which is still non-visible in the reflectance technique. In order to establish an alternative method for in vivo SPF testing, a huge number of sunscreens (80 samples) was measured by HDRS and compared to the worldwide accepted standard ISO 24444. The variety of sunscreens measured reflects a wide range of different types of formulations as well as a wide range of SPFs (5-120) to validate this new alternative SPF testing procedure. The applied quantity of product as well as skin color dependencies of signal generation are shown to support any basic correlation of DRS signal generation and sun protection expectations. Far-reaching statistical data analyses show an excellent link of the new non-erythemally driven HDRS-SPF technique and ISO 24444 results. In the same way, HDRS-UVA-PF results can be correlated with UVA-PF values calculated from ISO 24443. Due to the elimination of any erythemal relevant UVB and UVA doses, absolutely no skin reaction occurs. Consequently there is no need to define a MED any more. For the first time an alternative way to SPF is shown without any ethical concerns of SPF testing in vivo and/or any restriction of SPF testing in vitro. Regardless of the type of formulation or the level of protection, an excellent correlation of SPFHDRS and SPF24444 for sunscreen labeling could be found. By this new alternative non-erythemal technique, not only SPF values can be measured, but also UVA-PF values can be calculated with an excellent correlation to ISO 24443 from the same set of data. For the first time a robust alternative test method of SPF- and UVA-PF values is described, taking into account the interaction of sunscreen formulation and skin. © 2018 S. Karger AG, Basel.

Alternative Measurement Configurations for Extracting Bulk Optical Properties Using an Integrating Sphere Setup.

PubMed

Thennadil, Suresh N; Chen, Yi-Chieh

2017-02-01

The usual approach for estimating bulk optical properties using an integrating sphere measurement setup is by acquiring spectra from three measurement modes namely collimated transmittance (T c ), total transmittance (T d ), and total diffuse reflectance (R d ), followed by the inversion of these measurements using the adding-doubling method. At high scattering levels, accurate acquisition of T c becomes problematic due to the presence of significant amounts of forward-scattered light in this measurement which is supposed to contain only unscattered light. In this paper, we propose and investigate the effectiveness of using alternative sets of integrating sphere measurements that avoid the use of T c and could potentially increase the upper limit of concentrations of suspensions at which bulk optical property measurements can be obtained in the visible-near-infrared (Vis-NIR) region of the spectrum. We examine the possibility of replacing T c with one or more reflectance measurements at different sample thicknesses. We also examine the possibility of replacing both the collimated (T c ) and total transmittance (T d ) measurements with reflectance measurements taken from different sample thicknesses. The analysis presented here indicates that replacing T c with a reflectance measurement can reduce the errors in the bulk scattering properties when scattering levels are high. When only multiple reflectance measurements are used, good estimates of the bulk optical properties can be obtained when the absorption levels are low. In addition, we examine whether there is any advantage in using three measurements instead of two to obtain the reduced bulk scattering coefficient and the bulk absorption coefficient. This investigation is made in the context of chemical and biological suspensions which have a much larger range of optical properties compared to those encountered with tissue.

Relationship of 24-hour urinary free cortisol to 4-hour salivary morning and afternoon cortisol and cortisone as measured by a time-integrated oral diffusion sink.

PubMed

Kathol, R G; Poland, R E; Stokes, P E; Wade, S

1995-05-01

The relationship between salivary corticosteroids integrated over 4-hour periods and urinary free cortisol collected over 24 hours was investigated in normal controls. Twenty-one normal volunteers wore "oral diffusion sink" sampling devices in their mouths for two 4-hour periods (08:00-12:00 hours and 13:00-17:00 hours) and on the same day collected a 24-hour urine specimen. Time-integrated salivary corticosteroid concentrations were determined from the sample devices and urinary free cortisol was measured. Salivary corticosteroids were not consistently higher in the morning than in the afternoon period and did not differ between men and women. Urinary free cortisol levels were higher in women. No salivary corticosteroids measure was significantly correlated with urinary free cortisol. We conclude that time-integrated salivary corticosteroids do not reflect urinary free cortisol levels in normal controls.

White matter microstructure and volitional motor activity in schizophrenia: A diffusion kurtosis imaging study.

PubMed

Docx, Lise; Emsell, Louise; Van Hecke, Wim; De Bondt, Timo; Parizel, Paul M; Sabbe, Bernard; Morrens, Manuel

2017-02-28

Avolition is a core feature of schizophrenia and may arise from altered brain connectivity. Here we used diffusion kurtosis imaging (DKI) to investigate the association between white matter (WM) microstructure and volitional motor activity. Multi-shell diffusion MRI and 24-h actigraphy data were obtained from 20 right-handed patients with schizophrenia and 16 right-handed age and gender matched healthy controls. We examined correlations between fractional anisotropy (FA), mean diffusivity (MD), mean kurtosis (MK), and motor activity level, as well as group differences in these measures. In the patient group, increasing motor activity level was positively correlated with MK in the inferior, medial and superior longitudinal fasciculus, the corpus callosum, the posterior fronto-occipital fasciculus and the posterior cingulum. This association was not found in control subjects or in DTI measures. These results show that a lack of volitional motor activity in schizophrenia is associated with potentially altered WM microstructure in posterior brain regions associated with cognitive function and motivation. This could reflect both illness related dysconnectivity which through altered cognition, manifests as reduced volitional motor activity, and/or the effects of reduced physical activity on brain WM. Copyright © 2016. Published by Elsevier B.V.

Improved mathematical and computational tools for modeling photon propagation in tissue

NASA Astrophysics Data System (ADS)

Calabro, Katherine Weaver

Light interacts with biological tissue through two predominant mechanisms: scattering and absorption, which are sensitive to the size and density of cellular organelles, and to biochemical composition (ex. hemoglobin), respectively. During the progression of disease, tissues undergo a predictable set of changes in cell morphology and vascularization, which directly affect their scattering and absorption properties. Hence, quantification of these optical property differences can be used to identify the physiological biomarkers of disease with interest often focused on cancer. Diffuse reflectance spectroscopy is a diagnostic tool, wherein broadband visible light is transmitted through a fiber optic probe into a turbid medium, and after propagating through the sample, a fraction of the light is collected at the surface as reflectance. The measured reflectance spectrum can be analyzed with appropriate mathematical models to extract the optical properties of the tissue, and from these, a set of physiological properties. A number of models have been developed for this purpose using a variety of approaches -- from diffusion theory, to computational simulations, and empirical observations. However, these models are generally limited to narrow ranges of tissue and probe geometries. In this thesis, reflectance models were developed for a much wider range of measurement parameters, and influences such as the scattering phase function and probe design were investigated rigorously for the first time. The results provide a comprehensive understanding of the factors that influence reflectance, with novel insights that, in some cases, challenge current assumptions in the field. An improved Monte Carlo simulation program, designed to run on a graphics processing unit (GPU), was built to simulate the data used in the development of the reflectance models. Rigorous error analysis was performed to identify how inaccuracies in modeling assumptions can be expected to affect the accuracy of extracted optical property values from experimentally-acquired reflectance spectra. From this analysis, probe geometries that offer the best robustness against error in estimation of physiological properties from tissue, are presented. Finally, several in vivo studies demonstrating the use of reflectance spectroscopy for both research and clinical applications are presented.

Ultraviolet to optical diffuse sky emission as seen by the Hubble Space Telescope Faint Object Spectrograph

NASA Astrophysics Data System (ADS)

Kawara, Kimiaki; Matsuoka, Yoshiki; Sano, Kei; Brandt, Timothy D.; Sameshima, Hiroaki; Tsumura, Kohji; Oyabu, Shinki; Ienaka, Nobuyuki

2017-04-01

We present an analysis of the blank-sky spectra observed with the Faint Object Spectrograph on board the Hubble Space Telescope. We study the diffuse sky emission from ultraviolet to optical wavelengths, which is composed of zodiacal light (ZL), diffuse Galactic light (DGL), and residual emission. The observations were performed towards 54 fields distributed widely over the sky, with spectral coverage from 0.2 to 0.7 μm. In order to avoid contaminating light from earthshine, we use the data collected only in orbital nighttime. The observed intensity is decomposed into the ZL, DGL, and residual emission, in eight photometric bands spanning our spectral coverage. We found that the derived ZL reflectance spectrum is flat in the optical, which indicates major contribution of C-type asteroids to the interplanetary dust (IPD). In addition, the ZL reflectance spectrum has an absorption feature at ∼0.3 μm. The shape of the DGL spectrum is consistent with those found in earlier measurements and model predictions. While the residual emission contains a contribution from the extragalactic background light, we found that the spectral shape of the residual looks similar to the ZL spectrum. Moreover, its optical intensity is much higher than that measured from beyond the IPD cloud by Pioneer 10/11, and also than that of the integrated galaxy light. These findings may indicate the presence of an isotropic ZL component, which is missed in the conventional ZL models.

A fast calculating two-stream-like multiple scattering algorithm that captures azimuthal and elevation variations

NASA Astrophysics Data System (ADS)

Fiorino, Steven T.; Elmore, Brannon; Schmidt, Jaclyn; Matchefts, Elizabeth; Burley, Jarred L.

2016-05-01

Properly accounting for multiple scattering effects can have important implications for remote sensing and possibly directed energy applications. For example, increasing path radiance can affect signal noise. This study describes the implementation of a fast-calculating two-stream-like multiple scattering algorithm that captures azimuthal and elevation variations into the Laser Environmental Effects Definition and Reference (LEEDR) atmospheric characterization and radiative transfer code. The multiple scattering algorithm fully solves for molecular, aerosol, cloud, and precipitation single-scatter layer effects with a Mie algorithm at every calculation point/layer rather than an interpolated value from a pre-calculated look-up-table. This top-down cumulative diffusivity method first considers the incident solar radiance contribution to a given layer accounting for solid angle and elevation, and it then measures the contribution of diffused energy from previous layers based on the transmission of the current level to produce a cumulative radiance that is reflected from a surface and measured at the aperture at the observer. Then a unique set of asymmetry and backscattering phase function parameter calculations are made which account for the radiance loss due to the molecular and aerosol constituent reflectivity within a level and allows for a more accurate characterization of diffuse layers that contribute to multiple scattered radiances in inhomogeneous atmospheres. The code logic is valid for spectral bands between 200 nm and radio wavelengths, and the accuracy is demonstrated by comparing the results from LEEDR to observed sky radiance data.

Infrared deflectometry for the inspection of diffusely specular surfaces

NASA Astrophysics Data System (ADS)

Höfer, Sebastian; Burke, Jan; Heizmann, Michael

2016-12-01

Deflectometry is a full-field gradient technique that lends itself very well to testing specular surfaces. It uses the geometry of specular reflection to determine the gradient of the surface under inspection. In consequence, a necessary precondition to apply deflectometry is the presence of at least partially specular reflection. Surfaces with larger roughness have increasingly diffuse reflection characteristics, making them inaccessible to usual deflectometry. However, many industrially relevant surfaces exist that change their reflection characteristic during production and processing. An example is metal sheets that are used as car body parts. Whereas the molded but otherwise raw metal sheets show a mostly diffuse reflection without sufficient specular reflection, the final car body panels have a high specular reflectance due to the lacquering. In consequence, it would be advantageous to apply the same inspection approach both for the raw material and for the final product. To solve this challenge, specular reflection from rough surfaces can be achieved using light with a larger wavelength, as the specular reflectivity of a surface depends on the ratio of the surface roughness and the wavelength of the light applied. Wavelengths in the thermal infrared range create enough specular reflection to apply deflectometry on many visually rough metal surfaces. This contribution presents the principles of thermal deflectometry, its special challenges, and illustrates its use with examples from the inspection of industrially produced surfaces.

Longitudinal diffusion tensor magnetic resonance imaging study of radiation-induced white matter damage in a rat model.

PubMed

Wang, Silun; Wu, Ed X; Qiu, Deqiang; Leung, Lucullus H T; Lau, Ho-Fai; Khong, Pek-Lan

2009-02-01

Radiation-induced white matter (WM) damage is a major side effect of whole brain irradiation among childhood cancer survivors. We evaluate longitudinally the diffusion characteristics of the late radiation-induced WM damage in a rat model after 25 and 30 Gy irradiation to the hemibrain at 8 time points from 2 to 48 weeks postradiation. We hypothesize that diffusion tensor magnetic resonance imaging (DTI) indices including fractional anisotropy (FA), trace, axial diffusivity (lambda(//)), and radial diffusivity (lambda( perpendicular)) can accurately detect and monitor the histopathologic changes of radiation-induced WM damage, measured at the EC, and that these changes are dose and time dependent. Results showed a progressive reduction of FA, which was driven by reduction in lambda(//) from 4 to 40 weeks postradiation, and an increase in lambda( perpendicular) with return to baseline in lambda(//) at 48 weeks postradiation. Histologic evaluation of irradiated WM showed reactive astrogliosis from 4 weeks postradiation with reversal at 36 weeks, and demyelination, axonal degeneration, and necrosis at 48 weeks postradiation. Moreover, changes in lambda(//) correlated with reactive astrogliosis (P < 0.01) and lambda( perpendicular) correlated with demyelination (P < 0.01). Higher radiation dose (30 Gy) induced earlier and more severe histologic changes than lower radiation dose (25 Gy), and these differences were reflected by the magnitude of changes in lambda(//) and lambda( perpendicular). DTI indices reflected the histopathologic changes of WM damage and our results support the use of DTI as a biomarker to noninvasively monitor radiation-induced WM damage.

Self diffusion of interacting membrane proteins.

PubMed Central

Abney, J R; Scalettar, B A; Owicki, J C

1989-01-01

A two-dimensional version of the generalized Smoluchowski equation is used to analyze the time (or distance) dependent self diffusion of interacting membrane proteins in concentrated membrane systems. This equation provides a well established starting point for descriptions of the diffusion of particles that interact through both direct and hydrodynamic forces; in this initial work only the effects of direct interactions are explicitly considered. Data describing diffusion in the presence of hard-core repulsions, soft repulsions, and soft repulsions with weak attractions are presented. The effect that interactions have on the self-diffusion coefficient of a real protein molecule from mouse liver gap junctions is also calculated. The results indicate that self diffusion is always inhibited by direct interactions; this observation is interpreted in terms of the caging that will exist at finite protein concentration. It is also noted that, over small distance scales, the diffusion coefficient is determined entirely by the very strong Brownian forces; therefore, as a function of displacement the self-diffusion coefficient decays (rapidly) from its value at infinite dilution to its steady-state interaction-averaged value. The steady-state self-diffusion coefficient describes motion over distance scales that range from approximately 10 nm to cellular dimensions and is the quantity measured in fluorescence recovery after photobleaching experiments. The short-ranged behavior of the diffusion coefficient is important on the interparticle-distance scale and may therefore influence the rate at which nearest-neighbor collisional processes take place. The hard-disk theoretical results presented here are in excellent agreement with lattice Monte-Carlo results obtained by other workers. The concentration dependence of experimentally measured diffusion coefficients of antibody-hapten complexes bound to the membrane surface is consistent with that predicted by the theory. The variation in experimental diffusion coefficients of integral membrane proteins is greater than that predicted by the theory, and may also reflect protein-induced perturbations in membrane viscosity. PMID:2720077

Mean apparent propagator (MAP) MRI: a novel diffusion imaging method for mapping tissue microstructure.

PubMed

Özarslan, Evren; Koay, Cheng Guan; Shepherd, Timothy M; Komlosh, Michal E; İrfanoğlu, M Okan; Pierpaoli, Carlo; Basser, Peter J

2013-09-01

Diffusion-weighted magnetic resonance (MR) signals reflect information about underlying tissue microstructure and cytoarchitecture. We propose a quantitative, efficient, and robust mathematical and physical framework for representing diffusion-weighted MR imaging (MRI) data obtained in "q-space," and the corresponding "mean apparent propagator (MAP)" describing molecular displacements in "r-space." We also define and map novel quantitative descriptors of diffusion that can be computed robustly using this MAP-MRI framework. We describe efficient analytical representation of the three-dimensional q-space MR signal in a series expansion of basis functions that accurately describes diffusion in many complex geometries. The lowest order term in this expansion contains a diffusion tensor that characterizes the Gaussian displacement distribution, equivalent to diffusion tensor MRI (DTI). Inclusion of higher order terms enables the reconstruction of the true average propagator whose projection onto the unit "displacement" sphere provides an orientational distribution function (ODF) that contains only the orientational dependence of the diffusion process. The representation characterizes novel features of diffusion anisotropy and the non-Gaussian character of the three-dimensional diffusion process. Other important measures this representation provides include the return-to-the-origin probability (RTOP), and its variants for diffusion in one- and two-dimensions-the return-to-the-plane probability (RTPP), and the return-to-the-axis probability (RTAP), respectively. These zero net displacement probabilities measure the mean compartment (pore) volume and cross-sectional area in distributions of isolated pores irrespective of the pore shape. MAP-MRI represents a new comprehensive framework to model the three-dimensional q-space signal and transform it into diffusion propagators. Experiments on an excised marmoset brain specimen demonstrate that MAP-MRI provides several novel, quantifiable parameters that capture previously obscured intrinsic features of nervous tissue microstructure. This should prove helpful for investigating the functional organization of normal and pathologic nervous tissue. Copyright © 2013 Elsevier Inc. All rights reserved.

Constraining the Timescales of Rehydration in Nominally Anhydrous Minerals Using 3D Numerical Diffusion Models

NASA Astrophysics Data System (ADS)

Lynn, K. J.; Warren, J. M.

2017-12-01

Nominally anhydrous minerals (NAMs) are important for characterizing deep-Earth water reservoirs, but the water contents of olivine (ol), orthopyroxene (opx), and clinopyroxene (cpx) in peridotites generally do not reflect mantle equilibrium conditions. Ol is typically "dry" and decoupled from H in cpx and opx, which is inconsistent with models of partial melting and/or diffusive loss of H during upwelling beneath mid-ocean ridges. The rehydration of mantle pyroxenes via late-stage re-fertilization has been invoked to explain their relatively high water contents. Here, we use sophisticated 3D diffusion models (after Shea et al., 2015, Am Min) of H in ol, opx, and cpx to investigate the timescales of rehydration across a range of conditions relevant for melt-rock interaction and serpentinization of peridotites. Numerical crystals with 1 mm c-axis lengths and realistic crystal morphologies are modeled using recent H diffusivities that account for compositional variation and diffusion anisotropy. Models were run over timescales of minutes to millions of years and temperatures from 300 to 1200°C. Our 3D models show that, at the high-T end of the range, H concentrations in the cores of NAMs are partially re-equilibrated in as little as a few minutes, and completely re-equilibrated within hours to weeks. At low-T (300°C), serpentinization can induce considerable diffusion in cpx and opx. H contents are 30% re-equilibrated after continuous exposure to hydrothermal fluids for 102 and 105 years, respectively, which is inconsistent with previous interpretations that there is no effect on H in opx under similar conditions. Ol is unaffected after 1 Myr due to the slower diffusivity of the proton-vacancy mechanism at 300°C (2-4 log units lower than for opx). In the middle of the T range (700-1000°C), rehydration of opx and cpx occurs over hours to days, while ol is somewhat slower to respond (days to weeks), potentially allowing the decoupling observed in natural samples to occur via melt re-fertilization. Finally, off-center and oblique sections are common in natural samples and measurements likely reflect at least partially re-equilibrated compositions. Thus, the high water contents in peridotites may reflect variable NAM rehydration over a range of temperatures and timescales relevant for mid ocean ridge processes.

Development of a Model to Correct Multi-View Angle above Water Measurements for the Analysis of the Bidirectional Reflectance of Coral and Other Reef Substrates

NASA Astrophysics Data System (ADS)

Miller, I.; Forster, B. C.; Laffan, S. W.

2012-07-01

Spectral reflectance characteristics of substrates in a coral reef environment are often measured in the field by viewing a substrate at nadir. However, viewing a substrate from multiple angles would likely result in different spectral characteristics for most coral reef substrates and provide valuable information on structural properties. To understand the relationship between the morphology of a substrate and its spectral response it is necessary to correct the observed above-water radiance for the effects of atmosphere and water attenuation, at a number of view and azimuth angles. In this way the actual surface reflectance can be determined. This research examines the air-water surface interaction for two hypothetical atmospheric conditions (clear Rayleigh scattering and totally cloudcovered) and the global irradiance reaching the benthic surface. It accounts for both water scattering and absorption, with simplifications for shallow water conditions, as well as the additive effect of background reflectance being reflected at the water-air surface at angles greater than the critical refraction angle (~48°). A model was developed to correct measured above-water radiance along the refracted view angle for its decrease due to path attenuation and the "n squared law of radiance" and the additive surface reflectance. This allows bidirectional benthic surface reflectance and nadir-normalised reflectance to be determined. These theoretical models were adapted to incorporate above-water measures relative to a standard, diffuse, white reference panel. The derived spectral signatures of a number of coral and non-coral benthic surfaces compared well with other published results, and the signatures and nadir normalised reflectance of the corals and other benthic surface classes indicate good class separation.

Ultraviolet complex refractive index of Martian dust Laboratory measurements of terrestrial analogs

NASA Technical Reports Server (NTRS)

Egan, W. G.; Hilgeman, T.; Pang, K.

1975-01-01

The optical complex index of refraction of four candidate Martian surface materials has been determined between 0.185 and 0.4 microns using a modified Kubelka-Munk scattering theory. The cadidate materials were limonite, andesite, montmorillonite, and basalt. The effect of scattering has been removed from the results. Also presented are diffuse reflection and transmission data on these samples.

The structure of a magnetic-field front propagating non-diffusively in low-resistivity multi-species plasma

DOE Office of Scientific and Technical Information (OSTI.GOV)

Rubinstein, B.; Doron, R., E-mail: ramy.doron@weizmann.ac.il; Maron, Y.

2016-04-15

We report on the first experimental verification of the traveling-wave-like picture of a magnetic-field and an associated electric potential hill propagating non-diffusively in low resistivity plasma. High spatial resolution spectroscopic method, developed here, allowed for obtaining the detailed shape of the propagating magnetic-field front. The measurements demonstrated that the ion separation, previously claimed, results from the reflection of the higher charge-to-mass ratio ions from the propagating potential hill and from climbing the hill by the lower charge-to-mass ratio ions. This ion dynamics is found to be consistent with the observed electron density evolution.

Dependence of light scattering profile in tissue on blood vessel diameter and distribution: a computer simulation study.

PubMed

Duadi, Hamootal; Fixler, Dror; Popovtzer, Rachela

2013-11-01

Most methods for measuring light-tissue interactions focus on the volume reflectance while very few measure the transmission. We investigate both diffusion reflection and diffuse transmission at all exit angles to receive the full scattering profile. We also investigate the influence of blood vessel diameter on the scattering profile of a circular tissue. The photon propagation path at a wavelength of 850 nm is calculated from the absorption and scattering constants via Monte Carlo simulation. Several simulations are performed where a different vessel diameter and location were chosen but the blood volume was kept constant. The fraction of photons exiting the tissue at several central angles is presented for each vessel diameter. The main result is that there is a central angle that below which the photon transmission decreased for lower vessel diameters while above this angle the opposite occurred. We find this central angle to be 135 deg for a two-dimensional 10-mm diameter circular tissue cross-section containing blood vessels. These findings can be useful for monitoring blood perfusion and oxygen delivery in the ear lobe and pinched tissues. © 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)

Modeling and measuring extravascular hemoglobin: aging contusions

NASA Astrophysics Data System (ADS)

Lines, Collin; Kim, Oleg; Duffy, Susan; Alber, Mark; Crawford, Gregory P.

2011-07-01

Medical expertise is frequently elicited to aid in determining the age and the cause of the trauma or injury. Child protection and law enforcement frequently rely on the physical assessment of the trauma which involves delineating intentional from unintentional types of trauma. Recent studies have shown that current methods to assess the age of traumatic injuries are highly inaccurate and do not give reasonable predictions. Hemoglobin is one of the strongest chromophores in human tissues. Transport of hemoglobin and its breakdown products in tissue determines the spectrophotometric characteristics of the skin and its variations in time. Therefore, measurements of diffuse reflective spectra of the skin allow noninvasive screening. This paper reviews potential transmission and diffusive reflection spectroscopy based techniques and predictive and quantitative modeling methods assisting in efficient retrieval of the age of extravascular contusions. This paper then presents a novel Monte Carlo technique for 3D photon tracking and blood transport model. In future studies, clinically obtained spectra will be used to validate the model as well as fine-tune coefficients for absorption. It is the goal of this study to develop a model that would allow a non-invasive, accurate determination of the age of a bruise.

Fluorescence spectroscopy for endogenous porphyrins in human facial skin

NASA Astrophysics Data System (ADS)

Seo, I.; Tseng, S. H.; Cula, G. O.; Bargo, P. R.; Kollias, N.

2009-02-01

The activity of certain bacteria in skin is known to correlate to the presence of porphyrins. In particular the presence of coproporphyrin produced by P.acnes inside plugged pores has been correlated to acne vulgaris. Another porphyrin encountered in skin is protoporphyrin IX, which is produced by the body in the pathway for production of heme. In the present work, a fluorescence spectroscopy system was developed to measure the characteristic spectrum and quantify the two types of porphyrins commonly present in human facial skin. The system is comprised of a Xe lamp both for fluorescence excitation and broadband light source for diffuse reflectance measurements. A computer-controlled filter wheel enables acquisition of sequential spectra, first excited by blue light at 405 nm then followed by the broadband light source, at the same location. The diffuse reflectance spectrum was used to correct the fluorescence spectrum due to the presence of skin chromophores, such as blood and melanin. The resulting fluorescence spectra were employed for the quantification of porphyrin concentration in a population of healthy subjects. The results show great variability on the concentration of these porphyrins and further studies are being conducted to correlate them with skin conditions such as inflammation and acne vulgaris.

Physiological basis for noninvasive skin cancer diagnosis using diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Zhang, Yao; Markey, Mia K.; Tunnell, James W.

2017-02-01

Diffuse reflectance spectroscopy offers a noninvasive, fast, and low-cost alternative to visual screening and biopsy for skin cancer diagnosis. We have previously acquired reflectance spectra from 137 lesions in 76 patients and determined the capability of spectral diagnosis using principal component analysis (PCA). However, it is not well elucidated why spectral analysis enables tissue classification. To provide the physiological basis, we used the Monte Carlo look-up table (MCLUT) model to extract physiological parameters from those clinical data. The MCLUT model results in the following physiological parameters: oxygen saturation, hemoglobin concentration, melanin concentration, vessel radius, and scattering parameters. Physiological parameters show that cancerous skin tissue has lower scattering and larger vessel radii, compared to normal tissue. These results demonstrate the potential of diffuse reflectance spectroscopy for detection of early precancerous changes in tissue. In the future, a diagnostic algorithm that combines these physiological parameters could be enable non-invasive diagnosis of skin cancer.

Optical absorption and scattering spectra of pathological stomach tissues

NASA Astrophysics Data System (ADS)

Giraev, K. M.; Ashurbekov, N. A.; Lakhina, M. A.

2011-03-01

Diffuse reflection spectra of biotissues in vivo and transmission and reflection coefficients for biotissues in vitro are measured over 300-800 nm. These data are used to determine the spectral absorption and scattering indices and the scattering anisotropy factor for stomach mucous membranes under normal and various pathological conditions (chronic atrophic and ulcerous defects, malignant neoplasms). The most importan tphysiological (hemodynamic and oxygenation levels) and structural-morphological (scatterer size and density) parameters are also determined. The results of a morphofunctional study correlate well with the optical properties and are consistent with data from a histomorphological analysis of the corresponding tissues.

Sound propagation in street canyons: comparison between diffusely and geometrically reflecting boundaries

PubMed

Kang

2000-03-01

This paper systematically compares the sound fields in street canyons with diffusely and geometrically reflecting boundaries. For diffuse boundaries, a radiosity-based theoretical/computer model has been developed. For geometrical boundaries, the image source method has been used. Computations using the models show that there are considerable differences between the sound fields resulting from the two kinds of boundaries. By replacing diffuse boundaries with geometrical boundaries, the sound attenuation along the length becomes significantly less; the RT30 is considerably longer; and the extra attenuation caused by air or vegetation absorption is reduced. There are also some similarities between the sound fields under the two boundary conditions. For example, in both cases the sound attenuation along the length with a given amount of absorption is the highest if the absorbers are arranged on one boundary and the lowest if they are evenly distributed on all boundaries. Overall, the results suggest that, from the viewpoint of urban noise reduction, it is better to design the street boundaries as diffusely reflective rather than acoustically smooth.

Synergistic efficacy of salicylic acid with a penetration enhancer on human skin monitored by OCT and diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Zhao, Qingliang; Dai, Cuixia; Fan, Shanhui; Lv, Jing; Nie, Liming

2016-10-01

Salicylic acid (SA) has been frequently used as a facial chemical peeling agent (FCPA) in various cosmetics for facial rejuvenation and dermatological treatments in the clinic. However, there is a tradeoff between therapeutic effectiveness and possible adverse effects caused by this agent for cosmetologists. To optimize the cosmetic efficacy with minimal concentration, we proposed a chemical permeation enhancer (CPE) azone to synergistically work with SA on human skin in vivo. The optical properties of human skin after being treated with SA alone and SA combined with azone (SA@azone) were successively investigated by diffuse reflectance spectroscopy (DRS) and optical coherence tomography (OCT). Our results revealed that as the SA concentration increased, the light reflectance decreased and the absorption increased. We also found that SA@azone exhibited a synergistic effect on enhancing light penetration and OCT imaging depth. We demonstrated that the combination of DRS and OCT techniques could be used as a noninvasive, rapid and accurate measurement method to monitor the subtle changes of skin tissue after treatment with FCPA and CPE. The approach will greatly benefit the development of clinical cosmetic surgery, dermatosis diagnosis and therapeutic effect inspection in related biomedical studies.

Nondestructive Handheld Fourier Transform Infrared (FT-IR) Analysis of Spectroscopic Changes and Multivariate Modeling of Thermally Degraded Plain Portland Cement Concrete and its Slag and Fly Ash-Based Analogs.

PubMed

Leung Tang, Pik; Alqassim, Mohammad; Nic Daéid, Niamh; Berlouis, Leonard; Seelenbinder, John

2016-05-01

Concrete is by far the world's most common construction material. Modern concrete is a mixture of industrial pozzolanic cement formulations and aggregate fillers. The former acts as the glue or binder in the final inorganic composite; however, when exposed to a fire the degree of concrete damage is often difficult to evaluate nondestructively. Fourier transform infrared (FT-IR) spectroscopy through techniques such as transmission, attenuated total reflectance, and diffuse reflectance have been rarely used to evaluate thermally damaged concrete. In this paper, we report on a study assessing the thermal damage of concrete via the use of a nondestructive handheld FT-IR with a diffuse reflectance sample interface. In situ measurements can be made on actual damaged areas, without the need for sample preparation. Separate multivariate models were developed to determine the equivalent maximal temperature endured for three common industrial concrete formulations. The concrete mixtures were successfully modeled displaying high predictive power as well as good specificity. This has potential uses in forensic investigation and remediation services particularly for fires in buildings. © The Author(s) 2016.

Relationships between diffuse reflectance and vegetation canopy variables based on the radiative transfer theory

NASA Technical Reports Server (NTRS)

Park, J. K.; Deering, D. W.

1981-01-01

Out of the lengthy original expression of the diffuse reflectance formula, simple working equations were derived by employing characteristic parameters, which are independent of the canopy coverage and identifiable by field observations. The typical asymptotic nature of reflectance data that is usually observed in biomass studies was clearly explained. The usefulness of the simplified equations was demonstrated by the exceptionally close fit of the theoretical curves to two separately acquired data sets for alfalfa and shortgrass prairie canopies.

Diffusely Reflecting Paints Containing TFE

NASA Technical Reports Server (NTRS)

Shai, M. C.; Schutt, J. B.

1985-01-01

Highly reflective, diffused coatings developed by incorporating polytetrafluoroethylene (TFE) pigment with alcohol-soluble binders. Alcohol and binder mixed together in blender before adding TFE. TFE preferably outgassed in mechanical-pump vacuum for typical interval of 4 hours before adding to liquid. Like wetting agent, vacuum treatment helps to prevent clumping of TFE and eases dispersion throughout mixture. Mixture blended for 3 to 5 minutes before used. Coatings useful on reflectance-standard surfaces for calibrating radiometric instruments in both laboratory and field. Paints washable and usable as optical reference surfaces.

Analysis of direct to diffuse partitioning of global solar irradiance at the radiometric station in Badajoz (Spain)

NASA Astrophysics Data System (ADS)

Sanchez, G.; Cancillo, M. L.; Serrano, A.

2010-09-01

This study is aimed at the analysis of the partitioning of global solar irradiance into its direct and diffuse components at the radiometric station in Badajoz (Spain). The detailed knowledge of the solar radiation field is of increasing interest in Southern Europe due to its use as renewable energy. In particular, the knowledge of the solar radiation partitioning into direct and diffuse radiation has become a major demand for the design and suitable orientation of solar panels in solar power plants. In this study the first measurements of solar diffuse irradiance performed in the radiometric station in Badajoz (Spain) are presented and analyzed in the framework of the partitioning of solar global radiation. Thus, solar global and diffuse irradiance were measured at one-minute basis from 23 November 2009 to 31 March 2010. Solar irradiances were measured by two Kipp&Zonen CMP11 pyranometers, using a Kipp&Zonen CM121 shadow ring for the measurements of solar diffuse irradiance. Diffuse measurements were corrected from the solid angle hidden by the ring and direct irradiance was calculated as the difference between global and diffuse measurements. Irradiance was obtained from the pyranomenters by applying calibration coefficients obtained in an inter-comparison campaign performed at INTA/El Arenosillo, in Huelva (Spain), last September 2009. There, calibration coefficients were calculated using as a reference a CMP11 pyranometer which had been previously calibrated by the Physikalisch-Meteorologisches Observatorium Davos/World Radiation Centre in Switzerland. In order to study the partitioning of the solar radiation, the global and diffuse irradiances have been analyzed for three typical different sky conditions: cloud-free, broken clouds and overcast. Particular days within the period of study have been selected by visual inspection. Along with the analysis of the global and diffuse irradiances themselves, ratios of these irradiances to the downward irradiance at the top of the atmosphere have also been analyzed. Several interesting features have been found. It is particularly worth to note the decreasing relative contribution of the direct component to the global irradiance as the solar zenith angle increases, due to a longer path crossed within the atmosphere. In broken clouds and overcast conditions, the diffuse component becomes the major contribution to the irradiance being the high-frequency variability the main difference between both type of cases. While in overcast conditions the global irradiance remains remarkably low, under broken clouds the global irradiance shows a very high variability frequently reaching values higher than the irradiance at the top of the atmosphere, due to multi-reflection phenomenon. The present study contributes to a better knowledge of the radiation field and its partitioning, involving original high-frequency measurements.

Validation of Laser-Induced Fluorescent Photogrammetric Targets on Membrane Structures

NASA Technical Reports Server (NTRS)

Jones, Thomas W.; Dorrington, Adrian A.; Shortis, Mark R.; Hendricks, Aron R.

2004-01-01

The need for static and dynamic characterization of a new generation of inflatable space structures requires the advancement of classical metrology techniques. A new photogrammetric-based method for non-contact ranging and surface profiling has been developed at NASA Langley Research Center (LaRC) to support modal analyses and structural validation of this class of space structures. This full field measurement method, known as Laser-Induced Fluorescence (LIF) photogrammetry, has previously yielded promising experimental results. However, data indicating the achievable measurement precision had not been published. This paper provides experimental results that indicate the LIF-photogrammetry measurement precision for three different target types used on a reflective membrane structure. The target types were: (1) non-contact targets generated using LIF, (2) surface attached retro-reflective targets, and (3) surface attached diffuse targets. Results from both static and dynamic investigations are included.

Measuring Light Reflectance of BGO Crystal Surfaces

NASA Astrophysics Data System (ADS)

Janecek, Martin; Moses, William W.

2008-10-01

A scintillating crystal's surface reflectance has to be well understood in order to accurately predict and optimize the crystal's light collection through Monte Carlo simulations. In this paper, we measure the inner surface reflectance properties for BGO. The measurements include BGO crystals with a mechanically polished surface, rough-cut surface, and chemically etched surface, and with various reflectors attached, both air-coupled and with coupling compound. The measurements are performed with a laser aimed at the center of a hemispherical shaped BGO crystal. The hemispherical shape eliminates any non-perpendicular angles for light entering and exiting the crystal. The reflected light is collected with an array of photodiodes. The laser can be set at an arbitrary angle, and the photodiode array is rotated to fully cover 2pi of solid angle. The current produced in the photodiodes is readout with a digital multimeter connected through a multiplexer. The two rows of photodiodes achieve 5-degree by 4-degree resolution, and the current measurement has a dynamic range of 105:1. The acquired data was not described by the commonly assumed linear combination of specular and diffuse (Lambertian) distributions, except for a very few surfaces. Surface roughness proved to be the most important parameter when choosing crystal setup. The reflector choice was of less importance and of almost no consequence for rough-cut surfaces. Pure specular reflection distribution for all incidence angles was measured for polished surfaces with VM2000 film, while the most Lambertian distribution for any surface finish was measured for titanium dioxide paint. The distributions acquired in this paper will be used to create more accurate Monte Carlo models for light reflection distribution within BGO crystals.

Reflectivity Spectra for Commonly Used Reflectors

NASA Astrophysics Data System (ADS)

Janecek, Martin

2012-06-01

Monte Carlo simulations play an important role in developing and evaluating the performance of radiation detection systems. To accurately model a reflector in an optical Monte Carlo simulation, the reflector's spectral response has to be known. We have measured the reflection coefficient for many commonly used reflectors for wavelengths from 250 nm to 800 nm. The reflectors were also screened for fluorescence and angular distribution changes with wavelength. The reflectors examined in this work include several polytetrafluoroethylene (PTFE) reflectors, Spectralon, GORE diffuse reflector, titanium dioxide paint, magnesium oxide, nitrocellulose filter paper, Tyvek paper, Lumirror, Melinex, ESR films, and aluminum foil. All PTFE films exhibited decreasing reflectivity with longer wavelengths due to transmission. To achieve >;0.95 reflectivity in the 380 to 500 nm range, the PTFE films have to be at least 0.5 mm thick-nitrocellulose is a good alternative if a thin diffuse reflector is needed. Several of the reflectors have sharp declines in reflectivity below a cut-off wavelength, including TiO2 (420 nm), ESR film (395 nm), nitrocellulose (330 nm), Lumirror (325 nm), and Melinex (325 nm). PTFE-like reflectors were the only examined reflectors that had reflectivity above 0.90 for wavelengths below 300 nm. Lumirror, Melinex, and ESR film exhibited fluorescence. Lumirror and Melinex are excited by wavelengths between 320 and 420 nm and have their emission peaks located at 440 nm, while ESR film is excited by wavelengths below 400 nm and the emission peak is located at 430 nm. Lumirror and Melinex also exhibited changing angular distributions with wavelength.

Inferring ultraviolet anatomical exposure patterns while distinguishing the relative contribution of radiation components

NASA Astrophysics Data System (ADS)

Vuilleumier, Laurent; Milon, Antoine; Bulliard, Jean-Luc; Moccozet, Laurent; Vernez, David

2013-05-01

Exposure to solar ultraviolet (UV) radiation is the main causative factor for skin cancer. UV exposure depends on environmental and individual factors, but individual exposure data remain scarce. While ground UV irradiance is monitored via different techniques, it is difficult to translate such observations into human UV exposure or dose because of confounding factors. A multi-disciplinary collaboration developed a model predicting the dose and distribution of UV exposure on the basis of ground irradiation and morphological data. Standard 3D computer graphics techniques were adapted to develop a simulation tool that estimates solar exposure of a virtual manikin depicted as a triangle mesh surface. The amount of solar energy received by various body locations is computed for direct, diffuse and reflected radiation separately. Dosimetric measurements obtained in field conditions were used to assess the model performance. The model predicted exposure to solar UV adequately with a symmetric mean absolute percentage error of 13% and half of the predictions within 17% range of the measurements. Using this tool, solar UV exposure patterns were investigated with respect to the relative contribution of the direct, diffuse and reflected radiation. Exposure doses for various body parts and exposure scenarios of a standing individual were assessed using erythemally-weighted UV ground irradiance data measured in 2009 at Payerne, Switzerland as input. For most anatomical sites, mean daily doses were high (typically 6.2-14.6 Standard Erythemal Dose, SED) and exceeded recommended exposure values. Direct exposure was important during specific periods (e.g. midday during summer), but contributed moderately to the annual dose, ranging from 15 to 24% for vertical and horizontal body parts, respectively. Diffuse irradiation explained about 80% of the cumulative annual exposure dose.

Post launch performance of the Meteor-3/TOMS instrument

NASA Technical Reports Server (NTRS)

Jaross, Glen; Ahmad, Zia; Cebula, Richard P.; Krueger, Arlin J.

1994-01-01

The Meteor-3/TOMS instrument is the second in a series of Total Ozone Mapping Spectrometers (TOMS) following the 1978 launch of Nimbus-7/TOMS. TOMS instruments are designed to measure total ozone amounts over the entire earth on a daily basis, and have been the cornerstone of ozone trend monitoring. Consequently, calibration is a critical issue, and is receiving much attention on both instruments. Performance and calibration data obtained by monitoring systems aboard the Meteor-3 instrument have been analyzed through the first full year of operation, and indicate that the instrument is performing quite well. A new system for monitoring instrument sensitivity employing multiple diffusers has been used successfully and is providing encouraging results. The 3-diffuser system has monitored changes in instrument sensitivity of a few percent despite decreases in diffuser reflectivity approaching 50 percent since launch.

Influence of Energetic Disorder on Exciton Lifetime and Photoluminescence Efficiency in Conjugated Polymers.

PubMed

Rörich, Irina; Mikhnenko, Oleksandr V; Gehrig, Dominik; Blom, Paul W M; Crăciun, N Irina

2017-02-16

Using time-resolved photoluminescence (TRPL) spectroscopy the exciton lifetime in a range of conjugated polymers is investigated. For poly(p-phenylenevinylene) (PPV)-based derivatives and a polyspirobifluorene copolymer (PSBF) we find that the exciton lifetime is correlated with the energetic disorder. Better ordered polymers exhibit a single exponential PL decay with exciton lifetimes of a few hundred picoseconds, whereas polymers with a larger degree of disorder show multiexponential PL decays with exciton lifetimes in the nanosecond regime. These observations are consistent with diffusion-limited exciton quenching at nonradiative recombination centers. The measured PL decay time reflects the time that excitons need to diffuse toward these quenching sites. Conjugated polymers with large energetic disorder and thus longer exciton lifetime also exhibit a higher photoluminescence quantum yield due to the slower exciton diffusion toward nonradiative quenching sites.

Evaluation of diffuse-illumination holographic cinematography in a flutter cascade

NASA Technical Reports Server (NTRS)

Decker, A. J.

1986-01-01

Since 1979, the Lewis Research Center has examined holographic cinematography for three-dimensional flow visualization. The Nd:YAG lasers used were Q-switched, double-pulsed, and frequency-doubled, operating at 20 pulses per second. The primary subjects for flow visualization were the shock waves produced in two flutter cascades. Flow visualization was by diffuse-illumination, double-exposure, and holographic interferometry. The performances of the lasers, holography, and diffuse-illumination interferometry are evaluated in single-window wind tunnels. The fringe-contrast factor is used to evaluate the results. The effects of turbulence on shock-wave visualization in a transonic flow are discussed. The depth of field for visualization of a turbulent structure is demonstrated to be a measure of the relative density and scale of that structure. Other items discussed are the holographic emulsion, tests of coherence and polarization, effects of windows and diffusers, hologram bleaching, laser configurations, influence and handling of specular reflections, modes of fringe localization, noise sources, and coherence requirements as a function of the pulse energy. Holography and diffuse illumination interferometry are also reviewed.

Diffuse interstellar bands in reflection nebulae

NASA Technical Reports Server (NTRS)

Fischer, O.; Henning, Thomas; Pfau, Werner; Stognienko, R.

1994-01-01

A Monte Carlo code for radiation transport calculations is used to compare the profiles of the lambda lambda 5780 and 6613 Angstrom diffuse interstellar bands in the transmitted and the reflected light of a star embedded within an optically thin dust cloud. In addition, the behavior of polarization across the bands were calculated. The wavelength dependent complex indices of refraction across the bands were derived from the embedded cavity model. In view of the existence of different families of diffuse interstellar bands the question of other parameters of influence is addressed in short.

Bone optical spectroscopy for the measurement of hemoglobin content

NASA Astrophysics Data System (ADS)

Hollmann, Joseph L.; Arambel, Paula; Piet, Judith; Shefelbine, Sandra; Markovic, Stacey; Niedre, Mark; DiMarzio, Charles A.

2014-05-01

Osteoporosis is a common side effect of spinal cord injuries. Blood perfusion in the bone provides an indication of bone health and may help to evaluate therapies addressing bone loss. Current methods for measuring blood perfusion of bone use dyes and ionizing radiation, and yield qualitative results. We present a device capable of measuring blood oxygenation in the tibia. The device illuminates the skin directly over the tibia with a white light source and measures the diffusely reflected light in the near infrared spectrum. Multiple source-detector distances are utilized so that the blood perfusion in skin and bone may be differentiated.

Initial studies of the bidirectional reflectance distribution function of carbon nanotube structures for stray light control applications

NASA Astrophysics Data System (ADS)

Butler, James J.; Georgiev, Georgi T.; Tveekrem, June L.; Quijada, Manuel; Getty, Stephanie; Hagopian, John G.

2010-10-01

The Bidirectional Reflectance Distribution Function (BRDF) at visible and near-infrared wavelengths of Multi-Wall Carbon NanoTubes (MWCNTs) grown on substrate materials are reported. The BRDF measurements were performed in the Diffuser Calibration Laboratory (DCaL) at NASA's Goddard Space Flight Center, and results at 500nm and 900nm are reported here. In addition, the 8° Directional/Hemispherical Reflectance of the samples is reported from the ultraviolet to shortwave infrared. The 8° Directional/Hemispherical Reflectance was measured in the Optics Branch at NASA's Goddard Space Flight Center. The BRDF was measured at 0° and 45° incident angles and from -80° to +80° scatter angles using a monochromatic source. The optical scatter properties of the samples as represented by their BRDF were found to be strongly influenced by the choice of substrate. As a reference, the optical scattering properties of the carbon nanotubes are compared to the BRDF of Aeroglaze Z306TM and Rippey Ultrapol IVTM, a well-known black paint and black appliqué, respectively. The possibility, promise, and challenges of employing carefully engineered carbon nanotubes in straylight control applications particularly for spaceflight instrumentation is also discussed.

Feasibility of using diffuse reflectance spectroscopy for the quantification of brain edema

NASA Astrophysics Data System (ADS)

Rodriguez, Juan G.; Sisson, Cynthia; Hendricks, Chad; Pattillo, Chris; McWaters, Megan; Hardjasudarma, Mardjohan; Quarles, Chad; Yaroslavsky, Anna N.; Yaroslavsky, Ilya V.; Battarbee, Harold

2001-05-01

Many diseased states of the brain can result in the displacement of brain tissues and restrict cerebral blood flow, disrupting function in a life-threatening manner. Clinical examples where displacements are observed include venous thromboses, hematomas, strokes, tumors, abscesses, and, particularly, brain edema. For the latter, the brain tissue swells, displacing the cerebral spinal fluid (CSF) layer that surrounds it, eventually pressing itself against the skull. Under such conditions, catheters are often inserted into the brain's ventricles or the subarachnoid space to monitor increased pressure. These are invasive procedures that incur increased risk of infection and consequently are used reluctantly by clinicians. Recent studies in the field of biomedical optics have suggested that the presence or absence of the CSF layer can lead to dramatic changes in NIR signals obtained from diffuse reflectance measurements around the head. In this study, we consider how this sensitivity of NIR signals to CSF might be exploited to non-invasively monitor the onset and resolution of brain edema.

A hybrid sorption - Spectrometric method for determination of synthetic anionic dyes in foodstuffs.

PubMed

Tikhomirova, Tatyana I; Ramazanova, Gyulselem R; Apyari, Vladimir V

2017-04-15

A sorption-spectrometric method for determination of the anionic synthetic dyes based on their sorption on silica sorbent modified with hexadecyl groups (C16) followed by measuring the diffuse reflectance spectra on the surface of the sorbent has been proposed. Adsorption of sulfonated azo dyes Tartrazine (E102), Sunset Yellow FCF (E110), Ponceau 4R (E124) reaches maximum in acidic medium (1M HCl - pH 1). For the quinophthalone type dye Quinoline Yellow (E104), the adsorption is also maximal in an acidic medium (1M HCl - pH 2). The triphenylmethane dye Fast Green FCF (E143) is absorbed in the wider area of pH (1M HCl - pH 6). Increasing concentration of the dyes in a solution led to the increase in absorption band intensity in diffuse reflectance spectra of the adsorbent, which was used for their direct determination. The proposed method was applied to the determination of dyes in beverages and pharmaceuticals. Copyright © 2016 Elsevier Ltd. All rights reserved.

Suomi satellite brings to light a unique frontier of nighttime environmental sensing capabilities

PubMed Central

Miller, Steven D.; Mills, Stephen P.; Elvidge, Christopher D.; Lindsey, Daniel T.; Lee, Thomas F.; Hawkins, Jeffrey D.

2012-01-01

Most environmental satellite radiometers use solar reflectance information when it is available during the day but must resort at night to emission signals from infrared bands, which offer poor sensitivity to low-level clouds and surface features. A few sensors can take advantage of moonlight, but the inconsistent availability of the lunar source limits measurement utility. Here we show that the Day/Night Band (DNB) low-light visible sensor on the recently launched Suomi National Polar-orbiting Partnership (NPP) satellite has the unique ability to image cloud and surface features by way of reflected airglow, starlight, and zodiacal light illumination. Examples collected during new moon reveal not only meteorological and surface features, but also the direct emission of airglow structures in the mesosphere, including expansive regions of diffuse glow and wave patterns forced by tropospheric convection. The ability to leverage diffuse illumination sources for nocturnal environmental sensing applications extends the advantages of visible-light information to moonless nights. PMID:22984179

Surface diffusion of In on Ge(111) studied by optical second harmonic microscopy

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suni, I.I.; Seebauer, E.G.

Surface diffusion of In on Ge(111) has been measured by optical second harmonic microscopy. This technique employs surface second harmonic generation to directly image submonolayer surface concentration profiles. The coverage dependence of the diffusivity [ital D] can then be obtained from a Boltzmann--Matano analysis. In the coverage range 0.1[lt][theta][lt]0.48, the activation energy [ital E][sub diff] decreased with increasing coverage, ranging from 31 kcal/mol at [theta]=0.1 to 23 kcal/mol at [theta]=0.48. Over the same coverage range, the pre-exponential factor [ital D][sub 0] decreased from 5[times]10[sup 2] to 1[times]10[sup [minus]1] cm[sup 2]/s. This gradual change reflects a change in diffusion mechanism arisingmore » from the disordered nature of the Ge(111) surface. At low coverages, In adatoms sink into the top layer of Ge, and diffusion is dominated by thermal formation of adatom-vacancy pairs. At high coverages, diffusion occurs by normal site-to-site hopping. The gradual change in diffusion parameters with coverage was interrupted by an apparent phase transition at [theta]=0.16. At this point, both [ital E][sub diff] and [ital D][sub 0] peaked sharply at 41 kcal/mol and 6[times]10[sup 5] cm[sup 2]/s, respectively. The desorption energy [ital E][sub des] was measured by temperature programmed desorption. [ital E][sub des] decreased from 60 kcal/mol at submonolayer coverages to 55 kcal/mol at multilayer coverages.« less

Assessing Diffusion in the Extra-Cellular Space of Brain Tissue by Dynamic MRI Mapping of Contrast Agent Concentrations

NASA Astrophysics Data System (ADS)

Mériaux, Sébastien; Conti, Allegra; Larrat, Benoît

2018-05-01

The characterization of extracellular space (ECS) architecture represents valuable information for the understanding of transport mechanisms occurring in brain parenchyma. ECS tortuosity reflects the hindrance imposed by cell membranes to molecular diffusion. Numerous strategies have been proposed to measure the diffusion through ECS and to estimate its tortuosity. The first method implies the perfusion for several hours of a radiotracer which effective diffusion coefficient D* is determined after post mortem processing. The most well-established techniques are real-time iontophoresis that measures the concentration of a specific ion at known distance from its release point, and integrative optical imaging that relies on acquiring microscopy images of macromolecules labelled with fluorophore. After presenting these methods, we focus on a recent Magnetic Resonance Imaging (MRI)-based technique that consists in acquiring concentration maps of a contrast agent diffusing within ECS. Thanks to MRI properties, molecular diffusion and tortuosity can be estimated in 3D for deep brain regions. To further discuss the reliability of this technique, we point out the influence of the delivery method on the estimation of D*. We compare the value of D* for a contrast agent intracerebrally injected, with its value when the agent is delivered to the brain after an ultrasound-induced blood-brain barrier (BBB) permeabilization. Several studies have already shown that tortuosity may be modified in pathological conditions. Therefore, we believe that MRI-based techniques could be useful in a clinical context for characterizing the diffusion properties of pathological ECS and thus predicting the drug biodistribution into the targeted area.

High-b-value diffusion-weighted MR imaging for pretreatment prediction and early monitoring of tumor response to therapy in mice.

PubMed

Roth, Yiftach; Tichler, Thomas; Kostenich, Genady; Ruiz-Cabello, Jesus; Maier, Stephan E; Cohen, Jack S; Orenstein, Arie; Mardor, Yael

2004-09-01

To evaluate the use of diffusion-weighted magnetic resonance (MR) imaging with standard and high b values for pretreatment prediction and early detection of tumor response to various antineoplastic therapies in an animal model. Mice bearing C26 colon carcinoma tumors were treated with doxorubicin (n = 25) and with aminolevulinic acid-based photodynamic therapy (n = 23). Fourteen mice served as controls. Conventional T2-weighted fast spin-echo and diffusion-weighted MR images were acquired once before therapy and at 6, 24, and 48 hours after treatment. Pretreatment and early (1-2 days) posttreatment water diffusion parameters were calculated and compared with later changes in tumor volumes measured on conventional MR images by using the Pearson correlation test. In chemotherapy-treated tumors, a significant correlation (P <.002, r = 0.6) was observed between diffusion parameters that reflected tumor viability, measured prior to treatment, and changes in tumor volumes after therapy. This correlation implies that tumors with high pretreatment viability will respond better to chemotherapy than more necrotic tumors. In tumors treated with photodynamic therapy, no such correlation was found. Changes observed in water diffusion 1-2 days after treatment significantly correlated with later tumor growth rate for both therapies (P <.002, r = 0.54 for photodynamic therapy; P <.0003, r = 0.61 for chemotherapy). High-b-value diffusion-weighted MR imaging has potential use for the early detection of response to therapy and for predicting treatment outcome prior to initiation of chemotherapy. Copyright RSNA, 2004

Linear associations between clinically assessed upper motor neuron disease and diffusion tensor imaging metrics in amyotrophic lateral sclerosis.

PubMed

Woo, John H; Wang, Sumei; Melhem, Elias R; Gee, James C; Cucchiara, Andrew; McCluskey, Leo; Elman, Lauren

2014-01-01

To assess the relationship between clinically assessed Upper Motor Neuron (UMN) disease in Amyotrophic Lateral Sclerosis (ALS) and local diffusion alterations measured in the brain corticospinal tract (CST) by a tractography-driven template-space region-of-interest (ROI) analysis of Diffusion Tensor Imaging (DTI). This cross-sectional study included 34 patients with ALS, on whom DTI was performed. Clinical measures were separately obtained including the Penn UMN Score, a summary metric based upon standard clinical methods. After normalizing all DTI data to a population-specific template, tractography was performed to determine a region-of-interest (ROI) outlining the CST, in which average Mean Diffusivity (MD) and Fractional Anisotropy (FA) were estimated. Linear regression analyses were used to investigate associations of DTI metrics (MD, FA) with clinical measures (Penn UMN Score, ALSFRS-R, duration-of-disease), along with age, sex, handedness, and El Escorial category as covariates. For MD, the regression model was significant (p = 0.02), and the only significant predictors were the Penn UMN Score (p = 0.005) and age (p = 0.03). The FA regression model was also significant (p = 0.02); the only significant predictor was the Penn UMN Score (p = 0.003). Measured by the template-space ROI method, both MD and FA were linearly associated with the Penn UMN Score, supporting the hypothesis that DTI alterations reflect UMN pathology as assessed by the clinical examination.

Complex index of refraction estimation from degree of polarization with diffuse scattering consideration.

PubMed

Zhan, Hanyu; Voelz, David G; Cho, Sang-Yeon; Xiao, Xifeng

2015-11-20

The estimation of the refractive index from optical scattering off a target's surface is an important task for remote sensing applications. Optical polarimetry is an approach that shows promise for refractive index estimation. However, this estimation often relies on polarimetric models that are limited to specular targets involving single surface scattering. Here, an analytic model is developed for the degree of polarization (DOP) associated with reflection from a rough surface that includes the effect of diffuse scattering. A multiplicative factor is derived to account for the diffuse component and evaluation of the model indicates that diffuse scattering can significantly affect the DOP values. The scattering model is used in a new approach for refractive index estimation from a series of DOP values that involves jointly estimating n, k, and ρ(d)with a nonlinear equation solver. The approach is shown to work well with simulation data and additive noise. When applied to laboratory-measured DOP values, the approach produces significantly improved index estimation results relative to reference values.

Fine-pore aeration diffusers: accelerated membrane ageing studies.

PubMed

Kaliman, An; Rosso, Diego; Leu, Shao-Yuan; Stenstrom, Michael K

2008-01-01

Polymeric membranes are widely used in aeration systems for biological treatment. These membranes may degrade over time and are sensitive to fouling and scaling. Membrane degradation is reflected in a decline in operating performance and higher headloss, resulting in increased energy costs. Mechanical property parameters, such as membrane hardness, Young's modulus, and orifice creep, were used to characterize the performance of membranes over time in operation and to predict their failure. Used diffusers from municipal wastewater treatment plants were collected and tested for efficiency and headloss, and then dissected to facilitate measurements of Young's modulus, hardness, and orifice creep. Higher degree of membrane fouling corresponded consistently with larger orifice creep. A lab-scale membrane ageing simulation was performed with polyurethane and four different ethylene-propylene-diene (EPDM) membrane diffusers by subjecting them to chemical ageing cycles and periodic testing. The results confirmed full-scale plant results and showed the superiority of orifice creep over Young's modulus and hardness in predicting diffuser deterioration.

Reduced fractional anisotropy on diffusion tensor magnetic resonance imaging after hypoxic-ischemic encephalopathy.

PubMed

Ward, Phil; Counsell, Serena; Allsop, Joanna; Cowan, Frances; Shen, Yuji; Edwards, David; Rutherford, Mary

2006-04-01

Apparent diffusion coefficients (ADC) that are measured by diffusion-weighted imaging are reduced in severe white matter (WM) and in some severe basal ganglia and thalamic (BGT) injury in infants who present with hypoxic-ischemic encephalopathy (HIE). However, ADC values may pseudonormalize or even be high during this time in some less severe but clinically significant injuries. We hypothesized that fractional anisotropy (FA), a measure of the directional diffusivity of water made using diffusion tensor imaging, may be abnormal in these less severe injuries; therefore, the objective of this study was to use diffusion tensor imaging to measure ADC and FA in infants with moderate and severe hypoxic-ischemic brain injury. Twenty infants with HIE and 7 normal control infants were studied. All infants were born at >36 weeks' gestational age, and MRI scans were obtained within 3 weeks of delivery. Data were examined for normality, and comparisons were made using analysis of variance or Kruskal-Wallis as appropriate. During the first week, FA values were decreased with both severe and moderate WM and BGT injury as assessed by conventional imaging, whereas ADC values were reduced only in severe WM injury and some severe BGT injury. Abnormal ADC values pseudonormalized during the second week, whereas FA values continued to decrease. FA is reduced in moderate brain injury after HIE. A low FA may reflect a breakdown in WM organization. Moderate BGT injury may result in atrophy but not overt infarction; it is possible that delayed apoptosis is more marked than immediate necrosis, and this may account for normal early ADC values. The accompanying low FA within some severe and all moderate gray matter lesions, which is associated with significant later impairment, may help to confirm clinically significant abnormality in infants with normal ADC values.

MODIS Solar Diffuser On-orbit Performance

NASA Technical Reports Server (NTRS)

Xiong, Xiaoxiong; Chen, H.; Choi, T.; Sun, J.; Angal, A.

2008-01-01

MODIS is a key instrument for the NASA Earth Observing System (EOS), currently operated on both the Terra and Aqua missions. Each MODIS instrument has 20 reflective solar bands (RSBs) and 16 thermal emissive bands (TEBs). MODIS RSB on-orbit calibration is reflectance based using an on-board solar diffuser (SD). The SD bi-directional reflectance factors (BRFs) were characterized pre-launch using reference diffuser samples, which are traceable to NIST reflectance standards. The SD BRF on-orbit degradation (or change) is tracked by another onboard device, called the solar diffuser stability monitor (SDSM). The SDSM is operated during each scheduled SD calibration event, making alternate observations of direct sunlight and the diffusely reflected sunlight from the SD. The time series of the ratios of SDSM's SD view to its Sun view provide SD degradation information. This paper presents and compares the Terra and Aqua MODIS SD on-orbit performance. Results show that the SD on-orbit degradation depends on the amount of solar exposure of the SD plate. In addition, it is strongly wavelengthdependent, with a larger degradation rate at shorter wavelengths. For Terra MODIS, an SD door anomaly occurred in May 2003 that led to a decision to fix the door permanently at an "open" position. Since then, the SD degradation rate has significantly increased due to more frequent solar exposure. As expected, the SD on-orbit performance directly impacts the RSB calibration performance. The lessons learned from MODIS on-orbit calibration will provide useful insights into the development and operation of future SD calibration systems.

Reflectance spectroscopy can quantify cutaneous haemoglobin oxygenation by oxygen uptake from the atmosphere after epidermal barrier disruption.

PubMed

Heise, H M; Lampen, P; Stücker, M

2003-11-01

The supply of oxygen to the viable skin tissue within the upper layers is not only secured by the cutaneous blood vascular system, but to a significant part also by oxygen diffusion from the atmosphere through the horny layer. The aim of this study was to examine whether changes in haemoglobin oxygenation can be observed within the isolated perfused bovine udder skin used as a skin model by removing the upper horny layer by adhesive tape stripping. Diffuse reflectance spectroscopy in the visible spectral range was used for non-invasive characterisation of haemoglobin oxygenation in skin under in vitro conditions. Mid-infrared attenuated total reflectance spectroscopy was employed for analysing the surface layer of the stratum corneum with respect to keratin, water and lipid components. Skin barrier disruption was achieved by repeated stripping of superficial corneocyte layers by adhesive tape. Significant changes in skin haemoglobin oxygenation were observed for skin areas with reduced lipid concentration and a reduced stratum corneum layer, as determined from the quantitative evaluation of the diffuse reflectance skin spectra. The result can be interpreted as an increase of oxygen diffusion after the removal of the upper horny layer.

Role of Multiple Atmospheric Reflections in Formation of Electron Distribution Function in the Diffuse Aurora Region. Chapter 9

NASA Technical Reports Server (NTRS)

Khazanov, George V.; Himwich, Elizabeth W.; Glocer, Alex; Sibeck, David G.

2015-01-01

The precipitation of high-energy magnetospheric electrons (E greater than 500-600 electronvolts) in the diffuse aurora contributes significant energy flux into Earth's ionosphere. In the diffuse aurora, precipitating electrons initially injected from the plasmasheet via wave-particle interaction processes degrade in the atmosphere toward lower energies and produce secondary electrons via impact ionization of the neutral atmosphere. These initially precipitating electrons of magnetospheric origin can be additionally reflected back into the magnetosphere by the two magnetically conjugated atmospheres, leading to a series of multiple reflections that can greatly influence the initially precipitating flux at the upper ionospheric boundary (700-800 kilometers) and the resultant population of secondary electrons and electrons cascading toward lower energies. We present the solution of the Boltzmann.Landau kinetic equation that uniformly describes the entire electron distribution function in the diffuse aurora, including the affiliated production of secondary electrons (E is less than or equal to 600 electronvolts) and their energy interplay in the magnetosphere and two conjugated ionospheres. This solution takes into account the role of multiple atmospheric reflections of the precipitated electrons that were initially moved into the loss cone via wave.particle interaction processes in Earth's plasmasheet.

Spatially resolved, diffuse reflectance imaging for subsurface pattern visualization toward development of a lensless imaging platform: phantom experiments

NASA Astrophysics Data System (ADS)

Schelkanova, Irina; Pandya, Aditya; Saiko, Guennadi; Nacy, Lidia; Babar, Hannan; Shah, Duoaud; Lilge, Lothar; Douplik, Alexandre

2016-01-01

A portable, spatially resolved, diffuse reflectance lensless imaging technique based on the charge-coupled device or complementary metal-oxide semiconductor sensor directly coupled to the fiber optic bundle is proposed for visualization of subsurface structures such as superficial microvasculature in the epithelium. We discuss an experimental method for emulating a lensless imaging setup via raster scanning a single fiber-optic cable over a microfluidic phantom containing periodic hemoglobin absorption contrast. To evaluate the ability of the technique to recover information about the subsurface linear structures, scattering layers formed of the Sylgard® 184 Silicone Elastomer and titanium dioxide were placed atop the microfluidic phantom. Thickness of the layers ranged from 0.2 to 0.7 mm, and the values of the reduced scattering coefficient (μs‧) were between 0.85 and 4.25 mm-1. The results demonstrate that fiber-optic, lensless platform can be used for two-dimensional imaging of absorbing inclusions in diffuse reflectance mode. In these experiments, it was shown that diffuse reflectance imaging can provide sufficient spatial sampling of the phantom for differentiation of 30 μm structural features of the embedded absorbing pattern inside the scattering media.

ANALYTICAL SOLUTIONS OF THE ATMOSPHERIC DIFFUSION EQUATION WITH MULTIPLE SOURCES AND HEIGHT-DEPENDENT WIND SPEED AND EDDY DIFFUSIVITIES. (R825689C072)

EPA Science Inventory

Abstract

Three-dimensional analytical solutions of the atmospheric diffusion equation with multiple sources and height-dependent wind speed and eddy diffusivities are derived in a systematic fashion. For homogeneous Neumann (total reflection), Dirichlet (total adsorpti...

ANALYTICAL SOLUTIONS OF THE ATMOSPHERIC DIFFUSION EQUATION WITH MULTIPLE SOURCES AND HEIGHT-DEPENDENT WIND SPEED AND EDDY DIFFUSIVITIES. (R825689C048)

EPA Science Inventory

Abstract

Three-dimensional analytical solutions of the atmospheric diffusion equation with multiple sources and height-dependent wind speed and eddy diffusivities are derived in a systematic fashion. For homogeneous Neumann (total reflection), Dirichlet (total adsorpti...

Effect of cerebral spinal fluid suppression for diffusional kurtosis imaging.

PubMed

Yang, Alicia W; Jensen, Jens H; Hu, Caixia C; Tabesh, Ali; Falangola, Maria F; Helpern, Joseph A

2013-02-01

To evaluate the cerebral spinal fluid (CSF) partial volume effect on diffusional kurtosis imaging (DKI) metrics in white matter and cortical gray matter. Four healthy volunteers participated in this study. Standard DKI and fluid-attenuated inversion recovery (FLAIR) DKI experiments were performed using a twice-refocused-spin-echo diffusion sequence. The conventional diffusion tensor imaging (DTI) metrics of fractional anisotropy (FA), mean, axial, and radial diffusivity (MD, D[symbol in text], D[symbol in text] together with DKI metrics of mean, axial, and radial kurtosis (MK, K[symbol in text], K[symbol in text], were measured and compared. Single image slices located above the lateral ventricles, with similar anatomical features for each subject, were selected to minimize the effect of CSF from the ventricles. In white matter, differences of less than 10% were observed between diffusion metrics measured with standard DKI and FLAIR-DKI sequences, suggesting minimal CSF contamination. For gray matter, conventional DTI metrics differed by 19% to 52%, reflecting significant CSF partial volume effects. Kurtosis metrics, however, changed by 11% or less, indicating greater robustness with respect to CSF contamination. Kurtosis metrics are less sensitive to CSF partial voluming in cortical gray matter than conventional diffusion metrics. The kurtosis metrics may then be more specific indicators of changes in tissue microstructure, provided the effect sizes for the changes are comparable. Copyright © 2012 Wiley Periodicals, Inc.

NPP VIIRS and Aqua MODIS RSB Comparison Using Observations from Simultaneous Nadir Overpasses (SNO)

NASA Technical Reports Server (NTRS)

Xiong, X.; Wu, A.

2012-01-01

Suomi NPP (National Polar-orbiting Partnership) satellite (http://npp.gsfc.nasa.gov/viirs.html) began to daily collect global data following its successful launch on October 28, 2011. The Visible Infrared Imaging Radiometer Suite (VIIRS) is a key NPP sensor. Similar to the design of the OLS, SeaWiFS and MODIS instruments, VIIRS has on-board calibration components including a solar diffuser (SD) and a solar diffuser stability monitor (SDSM) for the reflective solar bands (RSB), a V-groove blackbody for the thermal emissive bands (TEB), and a space view (SV) port for background subtraction. Immediately after the VIIRS nadir door s opening on November 21, 2011, anomalously large degradation in the SD response was identified in the near-IR wavelength region, which was unexpected as decreases in the SD reflectance usually occur gradually in the blue (0.4 m) wavelength region based on past experience. In this study, we use a well-calibrated Aqua MODIS as reference to track and evaluate VIIRS RSB stability and performance. Reflectances observed by both sensors from simultaneous nadir overpasses (SNO) are used to determine VIIRS to MODIS reflectance ratios for their spectral matching bands. Results of this study provide an immediate post-launch assessment, independent validation of the anomalous degradation observed in SD measurements at near-IR wavelengths and initial analysis of calibration stability and consistency.

Development of X-ray laser media. Measurement of gain and development of cavity resonators for wavelengths near 130 angstroms, volume 3

NASA Astrophysics Data System (ADS)

Forsyth, J. M.

1983-02-01

In this document the authors summarize our investigation of the reflecting properties of X-ray multilayers. The breadth of this investigation indicates the utility of the difference equation formalism in the analysis of such structure. The formalism is particularly useful in analyzing multilayers whose structure is not a simple periodic bilayer. The complexity in structure can be either intentional, as in multilayers made by in-situ reflectance monitoring, or it can be a consequence of a degradation mechanism, such as random thickness errors or interlayer diffusion. Both the analysis of thickness errors and the analysis of interlayer diffusion are conceptually simple, effectively one dimensional problems that are straightforwared to pose. In the authors analysis of in-situ reflectance monitoring, they provide a quantitative understanding of an experimentally successful process that has not previously been treated theoretically. As X-ray multilayers come into wider use, there will undoubtedly be an increasing need for a more precise understanding of their reflecting properties. Thus, it is expected that in the future more detailed modeling will be undertaken of less easily specified structures than those above. The authors believe that their formalism will continue to prove useful in the modeling of these more complex structures. One such structure that may be of interest is that of a multilayer degraded by interfacial roughness.

Measurement of the ferric diffusion coefficient in agarose and gelatine gels by utilization of the evolution of a radiation induced edge as reflected in relaxation rate images.

PubMed

Pedersen, T V; Olsen, D R; Skretting, A

1997-08-01

A method has been developed to determine the diffusion coefficients of ferric ions in ferrous sulphate doped gels. A radiation induced edge was created in the gel, and two spin-echo sequences were used to acquire a pair of images of the gel at different points of time. For each of these image pairs, a longitudinal relaxation rate image was derived. From profiles through these images, the standard deviations of the Gaussian functions that characterize diffusion were determined. These data provided the basis for the determination of the ferric diffusion coefficients by two different methods. Simulations indicate that the use of single spin-echo images in this procedure may in some cases lead to a significant underestimation of the diffusion coefficient. The technique was applied to different agarose and gelatine gels that were prepared, irradiated and imaged simultaneously. The results indicate that the diffusion coefficient is lower in a gelatine gel than in an agarose gel. Addition of xylenol orange to a gelatine gel lowers the diffusion coefficient from 1.45 to 0.81 mm2 h-1, at the cost of significantly lower Rl sensitivity. The addition of benzoic acid to the latter gel did not increase the Rl sensitivity.

Monitoring of caffeine consumption effect on skin blood properties by diffuse reflectance spectroscopy

NASA Astrophysics Data System (ADS)

Milanic, Matija; Marin, Ana; Stergar, Jost; Verdel, Nina; Majaron, Boris

2017-07-01

Caffeine is the most widely consumed psychoactive substance in the world. It affects many tissues and organs, in particular central nervous system, heart, and blood vessels. The effect of caffeine on vascular smooth muscle cells is an initial transient contraction followed by significant vasodilatation. In this study we investigate the use of diffuse reflectance spectroscopy (DRS) for monitoring of vascular changes in human skin induced by caffeine consumption. DRS spectra were recorded on volar sides of the forearms of ten healthy volunteers at time delays of 0, 30, 60, 120, and 180 minutes after consumption of caffeine, while one subject served as a negative control. Analytical diffusion approximation solutions for diffuse reflectance from three-layer structures were used to assess skin composition (e.g., dermal blood volume fraction and oxygen saturation) by fitting to experimental data. The results demonstrate that cutaneous vasodynamics induced by caffeine consumption can be monitored by DRS, while changes in the control subject not consuming caffeine were insignificant.

Method for Calculating the Optical Diffuse Reflection Coefficient for the Ocular Fundus

NASA Astrophysics Data System (ADS)

Lisenko, S. A.; Kugeiko, M. M.

2016-07-01

We have developed a method for calculating the optical diffuse reflection coefficient for the ocular fundus, taking into account multiple scattering of light in its layers (retina, epithelium, choroid) and multiple refl ection of light between layers. The method is based on the formulas for optical "combination" of the layers of the medium, in which the optical parameters of the layers (absorption and scattering coefficients) are replaced by some effective values, different for cases of directional and diffuse illumination of the layer. Coefficients relating the effective optical parameters of the layers and the actual values were established based on the results of a Monte Carlo numerical simulation of radiation transport in the medium. We estimate the uncertainties in retrieval of the structural and morphological parameters for the fundus from its diffuse reflectance spectrum using our method. We show that the simulated spectra correspond to the experimental data and that the estimates of the fundus parameters obtained as a result of solving the inverse problem are reasonable.

Anti-collimation of ballistic electrons by a potential barrier

NASA Astrophysics Data System (ADS)

Coleridge, P. T.; Taylor, R. P.; Sachrajda, A. S.; Adams, J. A.

1994-03-01

A pair of Quantum Point Contacts separated by a continuous barrier have been fabricated using the surface gate technique. Transport measurements for each component of this system and for various combinations have shown both additive and non-additive behaviour. The results are explained by a combination of reflection by the barrier of electrons collimated by the Quantum Point Contacts and transport by diffusion across the barrier.

[Comparative study of reflectance spectroscopy of women's acupoints around menstruation].

PubMed

Jiang, Xing-Hui; Liu, Han-Ping; Guo, Zhou-Yi; Meng, Yao-Yong; Zeng, Chang-Chun; Liu, Song-Hao

2010-12-01

Acupoint was reaction of viscera and its optical parameter was an important characteristic of tissue. In the present work, in order to discuss specificity of acupuncture on the response of qi and blood in human body, we compared the diffuse reflectance of Taichong (LV3), Taibai (SP3), and Chongyang (ST42) before, during and after menstruation. All the acupoints had the same shape of the spectrum and troughs were all at 423.16, 544.06 and 577.47 nm. The values of reflectance during menstruation were greater than before and after menstruation, especially for SP3 and ST42 (P < 0.05). SP3 and ST42 were more sensitive to the changes of qi and blood than LV3, and it was asymmetric and we found no evidence for the imbalance of the left side and right. These results indicated that acupoint diffuse reflectance changed with qi and blood, and that SP3 and ST42 had close relationship with menstruation. Diffuse reflectance may be possibly used in the quantitative analysis of qi and blood.

Studying Room Acoustics using a Monopole-Dipole Microphone Array

NASA Technical Reports Server (NTRS)

Begault, Durand R.; Abel, Jonathan S.; Gills, Stephen R. (Technical Monitor)

1997-01-01

The use of a soundfield microphone for examining the directional nature of a room impulse response was reported recently. By cross-correlating monopole and co-located dipole microphone signals aligned with left-right, up-down, and front-back axes, a sense of signal direction of arrival is revealed. The current study is concerned with the array's ability to detect individual reflections and directions of arrival, as a function of the cross-correlation window duration. If is window is too long, weak reflections are overlooked; if too short, spurious detections result. Guidelines are presented for setting the window width according to perceptual criteria. Formulas are presented describing the accuracy with which direction of arrival can be estimated as a function of room specifics and measurement noise. The direction of arrival of early reflections is more accurately determined than that of later reflections which are quieter and more numerous. The transition from a fairly directional sound field at the beginning of the room impulse response to a uni-directional diffuse field is examined. Finally, it is shown that measurements from additional dipole orientations can significantly improve the ability to detect reflections and estimate their directions of arrival.

Homogenization of Electromagnetic and Seismic Wavefields for Joint Inverse Modeling

NASA Astrophysics Data System (ADS)

Newman, G. A.; Commer, M.; Petrov, P.; Um, E. S.

2011-12-01

A significant obstacle in developing a robust joint imaging technology exploiting seismic and electromagnetic (EM) wave fields is the resolution at which these different geophysical measurements sense the subsurface. Imaging of seismic reflection data is an order of magnitude finer in resolution and scale compared to images produced with EM data. A consistent joint image of the subsurface geophysical attributes (velocity, electrical conductivity) requires/demands the different geophysical data types be similar in their resolution of the subsurface. The superior resolution of seismic data results from the fact that the energy propagates as a wave, while propagation of EM energy is diffusive and attenuates with distance. On the other hand, the complexity of the seismic wave field can be a significant problem due to high reflectivity of the subsurface and the generation of multiple scattering events. While seismic wave fields have been very useful in mapping the subsurface for energy resources, too much scattering and too many reflections can lead to difficulties in imaging and interpreting seismic data. To overcome these obstacles a formulation for joint imaging of seismic and EM wave fields is introduced, where each data type is matched in resolution. In order to accomplish this, seismic data are first transformed into the Laplace-Fourier Domain, which changes the modeling of the seismic wave field from wave propagation to diffusion. Though high frequency information (reflectivity) is lost with this transformation, several benefits follow: (1) seismic and EM data can be easily matched in resolution, governed by the same physics of diffusion, (2) standard least squares inversion works well with diffusive type problems including both transformed seismic and EM, (3) joint imaging of seismic and EM data may produce better starting velocity models critical for successful reverse time migration or full waveform imaging of seismic data (non transformed) and (4) possibilities to image across multiple scale lengths, incorporating different types of geophysical data and attributes in the process. Important numerical details of 3D seismic wave field simulation in the Laplace-Fourier domain for both acoustic and elastic cases will also be discussed.

Sub-diffusive scattering parameter maps recovered using wide-field high-frequency structured light imaging.

PubMed

Kanick, Stephen Chad; McClatchy, David M; Krishnaswamy, Venkataramanan; Elliott, Jonathan T; Paulsen, Keith D; Pogue, Brian W

2014-10-01

This study investigates the hypothesis that structured light reflectance imaging with high spatial frequency patterns [Formula: see text] can be used to quantitatively map the anisotropic scattering phase function distribution [Formula: see text] in turbid media. Monte Carlo simulations were used in part to establish a semi-empirical model of demodulated reflectance ([Formula: see text]) in terms of dimensionless scattering [Formula: see text] and [Formula: see text], a metric of the first two moments of the [Formula: see text] distribution. Experiments completed in tissue-simulating phantoms showed that simultaneous analysis of [Formula: see text] spectra sampled at multiple [Formula: see text] in the frequency range [0.05-0.5] [Formula: see text] allowed accurate estimation of both [Formula: see text] in the relevant tissue range [0.4-1.8] [Formula: see text], and [Formula: see text] in the range [1.4-1.75]. Pilot measurements of a healthy volunteer exhibited [Formula: see text]-based contrast between scar tissue and surrounding normal skin, which was not as apparent in wide field diffuse imaging. These results represent the first wide-field maps to quantify sub-diffuse scattering parameters, which are sensitive to sub-microscopic tissue structures and composition, and therefore, offer potential for fast diagnostic imaging of ultrastructure on a size scale that is relevant to surgical applications.

Ultraviolet stability and contamination analysis of Spectralon diffuse reflectance material

NASA Technical Reports Server (NTRS)

Stiegman, Albert E.; Bruegge, Carol J.; Springsteen, Arthur W.

1993-01-01

A detailed chemical analysis was carried out on Spectralon, a highly Lambertian, diffuse reflectance material. Results of this investigation unambiguously identified the presence of an organic (hydrocarbon) impurity intrinsic to the commercial material. This impurity could be removed by a vacuum bake-out procedure and was identified as the cause of optical changes (degradation) that occur in the material when exposed to UV light. It was found that when this impurity was removed, the Spectralon material was photochemically stable and maintained its reflectance properties even after extensive solar UV exposure.

Suomi NPP VIIRS solar diffuser screen transmittance model and its applications.

PubMed

Lei, Ning; Xiong, Xiaoxiong; Mcintire, Jeff

2017-11-01

The visible infrared imaging radiometer suite on the Suomi National Polar-orbiting Partnership satellite calibrates its reflective solar bands through observations of a sunlit solar diffuser (SD) panel. Sunlight passes through a perforated plate, referred to as the SD screen, before reaching the SD. It is critical to know whether the SD screen transmittance measured prelaunch is accurate. Several factors such as misalignments of the SD panel and the measurement apparatus could lead to errors in the measured transmittance and thus adversely impact on-orbit calibration quality through the SD. We develop a mathematical model to describe the transmittance as a function of the angles that incident light makes with the SD screen, and apply the model to fit the prelaunch measured transmittance. The results reveal that the model does not reproduce the measured transmittance unless the size of the apertures in the SD screen is quite different from the design value. We attribute the difference to the orientation alignment errors for the SD panel and the measurement apparatus. We model the alignment errors and apply our transmittance model to fit the prelaunch transmittance to retrieve the "true" transmittance. To use this model correctly, we also examine the finite source size effect on the transmittance. Furthermore, we compare the product of the retrieved "true" transmittance and the prelaunch SD bidirectional reflectance distribution function (BRDF) value to the value derived from on-orbit data to determine whether the prelaunch SD BRDF value is relatively accurate. The model is significant in that it can evaluate whether the SD screen transmittance measured prelaunch is accurate and help retrieve the true transmittance from the transmittance with measurement errors, consequently resulting in a more accurate sensor data product by the same amount.

Lateral uniformity in chemical composition along a buried reaction front in polymers using off-specular reflectivity.

PubMed

Lavery, Kristopher A; Prabhu, Vivek M; Satija, Sushil; Wu, Wen-Li

2010-12-01

Off-specular neutron reflectometry was applied to characterize the form and amplitude of lateral compositional variations at a buried reaction-diffusion front. In this work, off-specular neutron measurements were first calibrated using off-specular x-ray reflectivity and atomic force microscopy via a roughened glass surface, both as a free surface and as a buried interface that was prepared by spin coating thin polymer films upon the glass surface. All three methods provided consistent roughness values despite the difference in their detection mechanism. Our neutron results demonstrated, for the first time, that the compositional heterogeneity at a buried reaction front can be measured; the model system used in this study mimics the deprotection reaction that occurs during the photolithographic process necessary for manufacturing integrated circuits.

Rapid determination of nitrites in food using a diffuse UV-visible reflectance method.

PubMed

Khan, S; Vila, M M D C; Tubino, M

2012-08-01

A simple, low-cost, sensitive and selective method for the determination of trace quantities of nitrite in foods such as cheese and cured meat using diffuse ultraviolet-visible reflectance was developed. It is based on the reaction of nitrite with sulphadiazine and α-naphthol, which produces a coloured product in basic medium. The reaction is carried out directly in the measuring cell. For cheese the limit of detection (LOD), expressed as NaNO₂, was estimated to be about 2.0 × 10⁻²mg l⁻¹ (2.9 × 10⁻⁷mol l⁻¹) in the final measuring solution and 0.17 mg kg⁻¹ in cheese (2.5 × 10⁻⁶mol kg⁻¹). The relative standard deviation (RSD) varied from 5% to 8% depending on the sample. For meat the LOD was estimated to be about 2.0 × 10⁻²mg l⁻¹ (2.9 × 10⁻⁷mol l⁻¹) in the final measuring solution and 0.13 mg kg⁻¹ in meat (1.9 × 10⁻⁶mol kg⁻¹). The RSD varied from 3% to 6% depending on the sample. The results of the proposed method were also compared with those obtained with the official method using the statistical Student's t-test and F-test procedures.

Multimodal optical setup based on spectrometer and cameras combination for biological tissue characterization with spatially modulated illumination

NASA Astrophysics Data System (ADS)

Baruch, Daniel; Abookasis, David

2017-04-01

The application of optical techniques as tools for biomedical research has generated substantial interest for the ability of such methodologies to simultaneously measure biochemical and morphological parameters of tissue. Ongoing optimization of optical techniques may introduce such tools as alternative or complementary to conventional methodologies. The common approach shared by current optical techniques lies in the independent acquisition of tissue's optical properties (i.e., absorption and reduced scattering coefficients) from reflected or transmitted light. Such optical parameters, in turn, provide detailed information regarding both the concentrations of clinically relevant chromophores and macroscopic structural variations in tissue. We couple a noncontact optical setup with a simple analysis algorithm to obtain absorption and scattering coefficients of biological samples under test. Technically, a portable picoprojector projects serial sinusoidal patterns at low and high spatial frequencies, while a spectrometer and two independent CCD cameras simultaneously acquire the reflected diffuse light through a single spectrometer and two separate CCD cameras having different bandpass filters at nonisosbestic and isosbestic wavelengths in front of each. This configuration fills the gaps in each other's capabilities for acquiring optical properties of tissue at high spectral and spatial resolution. Experiments were performed on both tissue-mimicking phantoms as well as hands of healthy human volunteers to quantify their optical properties as proof of concept for the present technique. In a separate experiment, we derived the optical properties of the hand skin from the measured diffuse reflectance, based on a recently developed camera model. Additionally, oxygen saturation levels of tissue measured by the system were found to agree well with reference values. Taken together, the present results demonstrate the potential of this integrated setup for diagnostic and research applications.

Effects of hydrogen peroxide on the light reflectance and morphology of bovine enamel.

PubMed

Kwon, Y H; Huo, M S; Kim, K H; Kim, S K; Kim, Y J

2002-05-01

The purpose of this study was to examine the effects of a bleaching agent (30% hydrogen peroxide) on the surface of bovine enamel using a scanning electron microscope and a UV-VIS-NIR spectrophotometer. Five non-carious bovine incisors were bleached for 0, 1, 2 and 3 days using 30% hydrogen peroxide. The light reflectance spectrum was measured using a spectrophotometer with diffuse reflectance mode. Colour values and colour differences in the teeth were evaluated from the reflectance measurements with the CIE L*a*b* colour coordinate system. Surface alterations in the bleached and unbleached teeth were studied using a scanning electron microscope. The change of reflectance in the teeth was related to the change of colour. Most reflectance change occurred within a 1-day bleaching, and this result was confirmed by a CIE L*a*b* colour coordinate system. The colour differences in the bleached teeth were significant enough to be perceived by the observer's eye. The comparison of bleached to unbleached bovine enamel revealed that the bleached surface showed non-uniform slight morphological alterations, and it developed varying degrees of surface porosity. This study indicates that the bleached bovine teeth showed apparent colour differences as well as slight morphological alterations after bleaching.

Radiometric Calibration of the Earth Observing System's Imaging Sensors

NASA Technical Reports Server (NTRS)

Slater, Philip N. (Principal Investigator)

1997-01-01

The work on the grant was mainly directed towards developing new, accurate, redundant methods for the in-flight, absolute radiometric calibration of satellite multispectral imaging systems and refining the accuracy of methods already in use. Initially the work was in preparation for the calibration of MODIS and HIRIS (before the development of that sensor was canceled), with the realization it would be applicable to most imaging multi- or hyper-spectral sensors provided their spatial or spectral resolutions were not too coarse. The work on the grant involved three different ground-based, in-flight calibration methods reflectance-based radiance-based and diffuse-to-global irradiance ratio used with the reflectance-based method. This continuing research had the dual advantage of: (1) developing several independent methods to create the redundancy that is essential for the identification and hopefully the elimination of systematic errors; and (2) refining the measurement techniques and algorithms that can be used not only for improving calibration accuracy but also for the reverse process of retrieving ground reflectances from calibrated remote-sensing data. The grant also provided the support necessary for us to embark on other projects such as the ratioing radiometer approach to on-board calibration (this has been further developed by SBRS as the 'solar diffuser stability monitor' and is incorporated into the most important on-board calibration system for MODIS)- another example of the work, which was a spin-off from the grant funding, was a study of solar diffuser materials. Journal citations, titles and abstracts of publications authored by faculty, staff, and students are also attached.

Quantitative broadband absorption and scattering spectroscopy in turbid media by combined frequency-domain and steady state methodologies

DOEpatents

Tromberg, Bruce J [Irvine, CA; Berger, Andrew J [Rochester, NY; Cerussi, Albert E [Lake Forest, CA; Bevilacqua, Frederic [Costa Mesa, CA; Jakubowski, Dorota [Irvine, CA

2008-09-23

A technique for measuring broadband near-infrared absorption spectra of turbid media that uses a combination of frequency-domain and steady-state reflectance methods. Most of the wavelength coverage is provided by a white-light steady-state measurement, whereas the frequency-domain data are acquired at a few selected wavelengths. Coefficients of absorption and reduced scattering derived from the frequency-domain data are used to calibrate the intensity of the steady-state measurements and to determine the reduced scattering coefficient at all wavelengths in the spectral window of interest. The absorption coefficient spectrum is determined by comparing the steady-state reflectance values with the predictions of diffusion theory, wavelength by wavelength. Absorption spectra of a turbid phantom and of human breast tissue in vivo, derived with the combined frequency-domain and steady-state technique, agree well with expected reference values.

Hyperspectral absorption and backscattering coefficients of bulk water retrieved from a combination of remote-sensing reflectance and attenuation coefficient.

PubMed

Lin, Junfang; Lee, Zhongping; Ondrusek, Michael; Liu, Xiaohan

2018-01-22

Absorption (a) and backscattering (bb) coefficients play a key role in determining the light field; they also serve as the link between remote sensing and concentrations of optically active water constituents. Here we present an updated scheme to derive hyperspectral a and bb with hyperspectral remote-sensing reflectance (Rrs) and diffuse attenuation coefficient (Kd) as the inputs. Results show that the system works very well from clear open oceans to highly turbid inland waters, with an overall difference less than 25% between these retrievals and those from instrument measurements. This updated scheme advocates the measurement and generation of hyperspectral a and bb from hyperspectral Rrs and Kd, as an independent data source for cross-evaluation of in situ measurements of a and bb and for the development and/or evaluation of remote sensing algorithms for such optical properties.

Diffusely reflecting paints including polytetrafluoroethylene and method of manufacture

NASA Technical Reports Server (NTRS)

Schutt, J. B.; Shai, M. C. (Inventor)

1985-01-01

The invention pertains to a high diffuse, reflective paint comprising an alcohol soluble binder, polytetrafluoroethylene (TFE) and an alcohol for coating a substrate and forming an optical reference with a superior Lambertian characteristic. A method for making the paint by first mixing the biner and alcohol, and thereafter by mixing in outgassed TFE is described. A wetting agent may be employed to aid the mixing process.

Improvement of direct determination of trace nickel in environmental samples by diffuse reflection spectroscopy using chromaticity characteristics.

PubMed

Ershova, N I; Ivanov, V M

2000-05-01

Cellulose and chromaton-N-super as solid supports for direct determination of the immobilized nickel complexes with dimethylglyoxime and benzyldioxime by diffuse reflection spectroscopy were compared. The advantage of chromaton-N-super with use of benzyldioxime is shown. Detection limit is 0.02 microg/mL. The proposed method was applied for the analysis of soil.

Experimental observation of an extremely dark material made by a low-density nanotube array.

PubMed

Yang, Zu-Po; Ci, Lijie; Bur, James A; Lin, Shawn-Yu; Ajayan, Pulickel M

2008-02-01

An ideal black material absorbs light perfectly at all angles and over all wavelengths. Here, we show that low-density vertically aligned carbon nanotube arrays can be engineered to have an extremely low index of refraction, as predicted recently by theory [Garcia-Vidal, F. J.; Pitarke, J. M.; Pendry, J. B. Phys. Rev. Lett. 1997, 78, 4289-4292] and, combined with the nanoscale surface roughness of the arrays, can produce a near-perfect optical absorption material. An ultralow diffused reflectance of 1 x 10(-7) measured from such arrays is an order-of-magnitude lower compared to commercial low-reflectance standard carbon. The corresponding integrated total reflectance of 0.045% from the nanotube arrays is three times lower than the lowest-ever reported values of optical reflectance from any material, making it the darkest man-made material ever.

Image statistics for surface reflectance perception.

PubMed

Sharan, Lavanya; Li, Yuanzhen; Motoyoshi, Isamu; Nishida, Shin'ya; Adelson, Edward H

2008-04-01

Human observers can distinguish the albedo of real-world surfaces even when the surfaces are viewed in isolation, contrary to the Gelb effect. We sought to measure this ability and to understand the cues that might underlie it. We took photographs of complex surfaces such as stucco and asked observers to judge their diffuse reflectance by comparing them to a physical Munsell scale. Their judgments, while imperfect, were highly correlated with the true reflectance. The judgments were also highly correlated with certain image statistics, such as moment and percentile statistics of the luminance and subband histograms. When we digitally manipulated these statistics in an image, human judgments were correspondingly altered. Moreover, linear combinations of such statistics allow a machine vision system (operating within the constrained world of single surfaces) to estimate albedo with an accuracy similar to that of human observers. Taken together, these results indicate that some simple image statistics have a strong influence on the judgment of surface reflectance.

Active hyperspectral imaging using a quantum cascade laser (QCL) array and digital-pixel focal plane array (DFPA) camera.

PubMed

Goyal, Anish; Myers, Travis; Wang, Christine A; Kelly, Michael; Tyrrell, Brian; Gokden, B; Sanchez, Antonio; Turner, George; Capasso, Federico

2014-06-16

We demonstrate active hyperspectral imaging using a quantum-cascade laser (QCL) array as the illumination source and a digital-pixel focal-plane-array (DFPA) camera as the receiver. The multi-wavelength QCL array used in this work comprises 15 individually addressable QCLs in which the beams from all lasers are spatially overlapped using wavelength beam combining (WBC). The DFPA camera was configured to integrate the laser light reflected from the sample and to perform on-chip subtraction of the passive thermal background. A 27-frame hyperspectral image was acquired of a liquid contaminant on a diffuse gold surface at a range of 5 meters. The measured spectral reflectance closely matches the calculated reflectance. Furthermore, the high-speed capabilities of the system were demonstrated by capturing differential reflectance images of sand and KClO₃ particles that were moving at speeds of up to 10 m/s.

Transport parameter estimation from lymph measurements and the Patlak equation.

PubMed

Watson, P D; Wolf, M B

1992-01-01

Two methods of estimating protein transport parameters for plasma-to-lymph transport data are presented. Both use IBM-compatible computers to obtain least-squares parameters for the solvent drag reflection coefficient and the permeability-surface area product using the Patlak equation. A matrix search approach is described, and the speed and convenience of this are compared with a commercially available gradient method. The results from both of these methods were different from those of a method reported by Reed, Townsley, and Taylor [Am. J. Physiol. 257 (Heart Circ. Physiol. 26): H1037-H1041, 1989]. It is shown that the Reed et al. method contains a systematic error. It is also shown that diffusion always plays an important role for transmembrane transport at the exit end of a membrane channel under all conditions of lymph flow rate and that the statement that diffusion becomes zero at high lymph flow rate depends on a mathematical definition of diffusion.

Ionic-to-Electronic Conductivity Crossover in CdTe-AgI-As2Te3 Glasses: An 110mAg Tracer Diffusion Study.

PubMed

Kassem, M; Alekseev, I; Bokova, M; Le Coq, D; Bychkov, E

2018-04-12

Conductivity isotherms of (CdTe) x (AgI) 0.5- x/2 (As 2 Te 3 ) 0.5- x/2 glasses (0.0 ≤ x ≤ 0.15) reveal a nonmonotonic behavior with increasing CdTe content reminiscent of mixed cation effect in oxide and chalcogenide glasses. Nevertheless, the apparent similarity appears to be partly incorrect. Using 110m Ag tracer diffusion measurements, we show that semiconducting CdTe additions produce a dual effect: (i) decreasing the Ag + ion transport by a factor of ≈200 with a simultaneous increase of the diffusion activation energy and (ii) increasing the electronic conductivity by 1.5 orders of magnitude. Consequently, the conductivity minimum at x = 0.05 reflects an ionic-to-electronic transport crossover; the silver-ion transport number decreases by 3 orders of magnitude with increasing x.

Polarimetric phenomenology in the reflective regime: a case study using polarized hyperspectral data

NASA Astrophysics Data System (ADS)

Gibney, Mark

2016-05-01

Understanding the phenomenology of polarimetric data is necessary if we want to obtain the maximum benefit when we exploit that data. To first order, polarimetric phenomenology is driven by two things; the target material type (specular or diffuse) and the illuminating source (point (sun) or extended (body emission)). Polarimetric phenomenology can then be broken into three basic categories; ([specular material/sun source], [diffuse/sun], [specular/body]) where we have assigned body emission to the IR passband where materials are generally specular. The task of interest determines the category of interest since the task determines the dominant target material and the illuminating source (eg detecting diffuse targets under trees in VNIR = [diffuse/sun] category). In this paper, a specific case study for the important [diffuse/sun] category will be presented. For the reflective regime (0.3 - 3.0um), the largest polarimetric signal is obtained when the sun illuminates a significant portion of the material BRDF lobe. This naturally points us to problems whose primary target materials are diffuse since the BRDF lobe for specular materials is tiny (low probability of acquiring on the BRDF lobe) and glinty (high probability of saturating the sensor when on lobe). In this case study, we investigated signatures of solar illuminated diffuse paints acquired by a polarimetric hyperspectral sensor. We will discuss the acquisition, reduction and exploitation of that data, and use it to illustrate the primary characteristics of reflective polarimetric phenomenology.

High sensitivity optical measurement of skin gloss

PubMed Central

Ezerskaia, Anna; Ras, Arno; Bloemen, Pascal; Pereira, Silvania F.; Urbach, H. Paul; Varghese, Babu

2017-01-01

We demonstrate a low-cost optical method for measuring the gloss properties with improved sensitivity in the low gloss regime, relevant for skin gloss properties. The gloss estimation method is based on, on the one hand, the slope of the intensity gradient in the transition regime between specular and diffuse reflection and on the other on the sum over the intensities of pixels above threshold, derived from a camera image obtained using unpolarized white light illumination. We demonstrate the improved sensitivity of the two proposed methods using Monte Carlo simulations and experiments performed on ISO gloss calibration standards with an optical prototype. The performance and linearity of the method was compared with different professional gloss measurement devices based on the ratio of specular to diffuse intensity. We demonstrate the feasibility for in-vivo skin gloss measurements by quantifying the temporal evolution of skin gloss after application of standard paraffin cream bases on skin. The presented method opens new possibilities in the fields of cosmetology and dermatopharmacology for measuring the skin gloss and resorption kinetics and the pharmacodynamics of various external agents. PMID:29026683

High sensitivity optical measurement of skin gloss.

PubMed

Ezerskaia, Anna; Ras, Arno; Bloemen, Pascal; Pereira, Silvania F; Urbach, H Paul; Varghese, Babu

2017-09-01

We demonstrate a low-cost optical method for measuring the gloss properties with improved sensitivity in the low gloss regime, relevant for skin gloss properties. The gloss estimation method is based on, on the one hand, the slope of the intensity gradient in the transition regime between specular and diffuse reflection and on the other on the sum over the intensities of pixels above threshold, derived from a camera image obtained using unpolarized white light illumination. We demonstrate the improved sensitivity of the two proposed methods using Monte Carlo simulations and experiments performed on ISO gloss calibration standards with an optical prototype. The performance and linearity of the method was compared with different professional gloss measurement devices based on the ratio of specular to diffuse intensity. We demonstrate the feasibility for in-vivo skin gloss measurements by quantifying the temporal evolution of skin gloss after application of standard paraffin cream bases on skin. The presented method opens new possibilities in the fields of cosmetology and dermatopharmacology for measuring the skin gloss and resorption kinetics and the pharmacodynamics of various external agents.

Optical Distance Measurement Device And Method Thereof

DOEpatents

Bowers, Mark W.

2004-06-15

A system and method of efficiently obtaining distance measurements of a target by scanning the target. An optical beam is provided by a light source and modulated by a frequency source. The modulated optical beam is transmitted to an acousto-optical deflector capable of changing the angle of the optical beam in a predetermined manner to produce an output for scanning the target. In operation, reflected or diffused light from the target may be received by a detector and transmitted to a controller configured to calculate the distance to the target as well as the measurement uncertainty in calculating the distance to the target.

Thermal characteristics of the lunar surface layer.

NASA Technical Reports Server (NTRS)

Cremers, C. J.; Birkebak, R. C.; White, J. E.

1972-01-01

The thermophysical properties of the fines from the Apollo 12 landing site have been determined as a function of their relevant parameters. These properties include the thermal conductivity, thermal diffusivity, directional reflectance and emittance. The density used was the same as that observed from the returned core-tube samples and so should be close to the true density of the surface layer at the Apollo 12 site. The measured properties are used to calculate the diurnal temperature variation of the moon's surface as well as for several depths below the surface. The maximum surface of 389 K is obtained at lunar noon while the minimum temperature of 86.1 K is obtained at sunrise. It is shown that the most significant effects on temperature, as compared with previous calculations, are caused by using the directional reflectance which controls the amount of solar energy absorption during the day in place of a constant hemispherical reflectance. The results are compared with previous analyses and remote measurements.

Non-Implanted Gallium-Arsenide and its Subsequent Annealing Effects.

NASA Astrophysics Data System (ADS)

Liou, Lih-Yeh

Infrared spectroscopy is used to study ion-implanted GaAs and its subsequent annealing effects. The damage in the implantation region causes a change in dielectric constant resulting in an infrared reflection spectrum which shows the interference pattern of a multilayer structure. Reflection data are fitted by values calculated from a physically realistic model by using computer codes. The first part in this work studies the solid state regrowth of amorphous GaAs made by Be implantation at -100(DEGREES)C. The regrowth temperature is around 200(DEGREES)C. The regrowth starts with a narrowing of the transition region and the transformation of the implanted layer from as-implanted amorphous (a-l) state to thermally-stablized amorphous (a-ll) state. The non-epitaxial recrystallization from both the surface and the interfacial region follows. The final regrown layer has a slightly higher refractive index than the crystalline value, indicating a high residual defect concentration. The temperature dependent regrowth velocity and the activation energy for this process are determined. The second part studies the free carrier activation in Be-implanted GaAs. Free holes are activated with prolonged annealing at 400(DEGREES)C ((TURN)50 hours) or a shorter time at higher temperature. The carrier contribution to the dielectric constant is calculated from the classical model and best fit to the reflection results show that the carrier profile can be approximated by a two half-Gaussians joined smoothly at their peaks. The peak position for the profile occurs deeper than that for the Be impurity profile measured by SIMS. The carrier distribution is speculated to be the result of the Be impurity, Ga vacancy and possible compensating defect distributions. The final part studies the free carrier removal by proton implantation in heavily doped, high carrier density, n-type GaAs. The as-implantation region is highly compensated until annealed at 550(DEGREES)C. After annealing between 300 and 400(DEGREES)C, the infrared results show a partially compensated region diffused deeply into substrate from the as-implanted region. The SIMS measurements show a well correlated hydrogen diffusion layer which suggests that the compensation defect is hydrogen related. After 500(DEGREES)C, the hydrogen diffusion layer is still observed, but the compensation layer has disappeared. The diffusion coefficient of the compensating defect and the activation energy for this process are determined. Carbon -implanted GaAs having a high carrier density substrate is also measured and compared with the H-implanted cases. (Copies available exclusively from Micrographics Department, Doheny Library, USC, Los Angeles, CA 90089 -0182.).

Prepreg cure monitoring using diffuse reflectance-FTIR. [Fourier Transform Infrared Technique

NASA Technical Reports Server (NTRS)

Young, P. R.; Chang, A. C.

1984-01-01

An in situ diffuse reflectance-Fourier transform infrared technique was developed to determine infrared spectra of graphite fiber prepregs as they were being cured. A bismaleimide, an epoxy, and addition polyimide matrix resin prepregs were studied. An experimental polyimide adhesive was also examined. Samples were positioned on a small heater at the focal point of diffuse reflectance optics and programmed at 15 F/min while FTIR spectra were being scanned, averaged, and stored. An analysis of the resulting spectra provided basic insights into changes in matrix resin molecular structure which accompanied reactions such as imidization and crosslinking. An endo-exothermal isomerization involving reactive end-caps was confirmed for the addition polyimide prepregs. The results of this study contribute to a fundamental understanding of the processing of composites and adhesives. Such understanding will promote the development of more efficient cure cycles.

Spectral Reflectance Measurement of Evaporating Chemical Films: Initial Results and Application to Skin Permeation.

PubMed

Frasch, H Frederick; Lee, Larry; Barbero, Ana M

2018-04-27

The current study has two aims. First the method of spectral reflectance was used to measure evaporation rates of thin (∼25-300 μm) films of neat liquid volatile organic chemicals exposed to a well-regulated wind speed u. Gas phase evaporation mass transfer coefficient (k evap ) measurements of 10 chemicals, 9 of which were measured at similar u, are predicted (slope of log-log data = 1.01; intercept = 0.08; R 2 = 0.996) by a previously proposed mass transfer correlation. For one chemical, isoamyl alcohol, the dependence of k evap on u 0.52 was measured, in support of the predicted exponent value of ½. Second, measured k evap of nicotine was used as an input in analytical models based on diffusion theory to estimate the absorbed fraction (F abs ) of a small dose (5 μL/cm 2 ) applied to human epidermis in vitro. The measured F abs was 0.062 ± 0.023. Model-estimated values are 0.066 and 0.115. Spectral reflectance is a precise method of measuring k evap of liquid chemicals and the data are well-described by a simple gas phase mass transfer coefficient. For nicotine under the single exposure condition measured herein, F abs is well-predicted from a theoretical model that requires knowledge of k evap , maximal dermal flux and membrane lag time. Copyright © 2018. Published by Elsevier Inc.

Carrier diffusion as a measure of carrier/exciton transfer rate in InAs/InGaAsP/InP hybrid quantum dot-quantum well structures emitting at telecom spectral range

NASA Astrophysics Data System (ADS)

Rudno-Rudziński, W.; Biegańska, D.; Misiewicz, J.; Lelarge, F.; Rousseau, B.; Sek, G.

2018-01-01

We investigate the diffusion of photo-generated carriers (excitons) in hybrid two dimensional-zero dimensional tunnel injection structures, based on strongly elongated InAs quantum dots (called quantum dashes, QDashes) of various heights, designed for emission at around 1.5 μm, separated by a 3.5 nm wide barrier from an 8 nm wide In0.64Ga0.36As0.78P0.22 quantum well (QW). By measuring the spectrally filtered real space images of the photoluminescence patterns with high resolution, we probe the spatial extent of the emission from QDashes. Deconvolution with the exciting light spot shape allows us to extract the carrier/exciton diffusion lengths. For the non-resonant excitation case, the diffusion length depends strongly on excitation power, pointing at carrier interactions and phonons as its main driving mechanisms. For the case of excitation resonant with absorption in the adjacent QW, the diffusion length does not depend on excitation power for low excitation levels since the generated carriers do not have sufficient excess kinetic energy. It is also found that the diffusion length depends on the quantum-mechanical coupling strength between QW and QDashes, controlled by changing the dash size. It influences the energy difference between the QDash ground state of the system and the quantum well levels, which affects the tunneling rates. When that QW-QDash level separation decreases, the probability of capturing excitons generated in the QW by QDashes increases, which is reflected by the decreased diffusion length from approx. 5 down to 3 μm.

Ground truth data for test sites (SL-4). [thermal radiation brightness temperature and solar radiation measurments

NASA Technical Reports Server (NTRS)

1974-01-01

Field measurements performed simultaneous with Skylab overpass in order to provide comparative calibration and performance evaluation measurements for the EREP sensors are presented. Wavelength region covered include: solar radiation (400 to 1300 nanometer), and thermal radiation (8 to 14 micrometer). Measurements consisted of general conditions and near surface meteorology, atmospheric temperature and humidity vs altitude, the thermal brightness temperature, total and diffuse solar radiation, direct solar radiation (subsequently analyzed for optical depth/transmittance), and target reflectivity/radiance. The particular instruments used are discussed along with analyses performed. Detailed instrument operation, calibrations, techniques, and errors are given.

Comparative study of radiometric and calorimetric methods for total hemispherical emissivity measurements

NASA Astrophysics Data System (ADS)

Monchau, Jean-Pierre; Hameury, Jacques; Ausset, Patrick; Hay, Bruno; Ibos, Laurent; Candau, Yves

2018-05-01

Accurate knowledge of infrared emissivity is important in applications such as surface temperature measurements by infrared thermography or thermal balance for building walls. A comparison of total hemispherical emissivity measurement was performed by two laboratories: the Laboratoire National de Métrologie et d'Essais (LNE) and the Centre d'Études et de Recherche en Thermique, Environnement et Systèmes (CERTES). Both laboratories performed emissivity measurements on four samples, chosen to cover a large range of emissivity values and angular reflectance behaviors. The samples were polished aluminum (highly specular, low emissivity), bulk PVC (slightly specular, high emissivity), sandblasted aluminum (diffuse surface, medium emissivity), and aluminum paint (slightly specular surface, medium emissivity). Results obtained using five measurement techniques were compared. LNE used a calorimetric method for direct total hemispherical emissivity measurement [1], an absolute reflectometric measurement method [2], and a relative reflectometric measurement method. CERTES used two total hemispherical directional reflectometric measurement methods [3, 4]. For indirect techniques by reflectance measurements, the total hemispherical emissivity values were calculated from directional hemispherical reflectance measurement results using spectral integration when required and directional to hemispherical extrapolation. Results were compared, taking into account measurement uncertainties; an added uncertainty was introduced to account for heterogeneity over the surfaces of the samples and between samples. All techniques gave large relative uncertainties for a low emissive and very specular material (polished aluminum), and results were quite scattered. All the indirect techniques by reflectance measurement gave results within ±0.01 for a high emissivity material. A commercial aluminum paint appears to be a good candidate for producing samples with medium level of emissivity (about 0.4) and with good uniformity of emissivity values (within ±0.015).

Optical properties of human colon tissues in the 350 – 2500 nm spectral range

DOE Office of Scientific and Technical Information (OSTI.GOV)

Bashkatov, A N; Genina, E A; Kochubey, V I

2014-08-31

We present the optical characteristics of the mucosa and submucosa of human colon tissue. The experiments are performed in vitro using a LAMBDA 950 spectrophotometer in the 350 – 2500 nm spectral range. The absorption and scattering coefficients and the scattering anisotropy factor are calculated based on the measured diffuse reflectance and total and collimated transmittance spectra using the inverse Monte Carlo method. (laser biophotonics)

Miniature and Molecularly Specific Optical Screening Technologies for Breast Cancer

DTIC Science & Technology

2008-10-01

commercially available dual-channel transimpedance amplifier circuit boards (Boston Electronics, TWAMP). Preliminary results with the imaging probe...connected to a current amplifier via a coaxial cable for diffuse reflectance measurements. This new probe is named P4-3 and schematics of the system and...probe. With the single pixel device a single-channel current amplifier (Terahertz Technologies, PDA-750) could easily read and collect the photocurrent

Low-Angle Radar Tracking

DTIC Science & Technology

1976-02-01

Transition from Specular Reflection to Diffuse Scattering. . . 10 Composition of the Electric-Field Vector as Seen at the Radar...r t (16) R • FIGURE P COMPOSITION OF THE ELECTRIC-FIELD VECTOR AS SEEN AT THE RADAR, R, IN FIG. 2. The electric field at the radar, E, is the sum...wavelengths in the VHP and UHF ranges even subsurface characteristics can be important. So in a field experiment one must be careful to measure

Diffusion reflection technique for diagnosis of atherosclerosis in mice using gold nanorods

NASA Astrophysics Data System (ADS)

Chakraborty, Ruchira; Ankri, Rinat; Leshem-Lev, Dorit; Lev, Eli I.; Hochhauser, Edith; Motiei, Menachem; Fixler, Dror

2018-02-01

Atherosclerosis, the leading cause of morbidity and mortality of cardiovascular disease, occur due to hardening and narrowing of arteries for development of vulnerable plaques made of cholesterols, tissue macrophages, foam cells and smooth muscle cells. Early detection of atherosclerosis is essential for proper treatment. Our group has already reported about the potential application of the non-invasive diffusion reflection (DR) technique in the detection of atherosclerosis using gold nanorods (GNRs) as contrast agent in carotid artery injured mice model. The basics of the study lie on the uptake GNRs by macrophages that located at the vulnerable plaques, which act as a good absorption contrast for DR measurement. Accumulations of GNRs cause changes in the optical property of the tissues and in turn cause changes in DR profile. In this study, we report the potential application of DR measurement in the detection of atherosclerosis in high-fat diet mice. Here, we have used PEG-coated GNRs with absorption maxima around 660nm. The time kinetics showed that after 24h of GNR injection the DR can find the atherosclerotic plaques and with time (up to 72h) the GNR accumulation in plaques were faded out, but still can be detectable by DR. Our result strongly suggests that in future DR can be used for early detection of atherosclerosis.

Determination of band gap in epitaxial delafossite Cu oxide using optical techniques

NASA Astrophysics Data System (ADS)

Cabrera, Alejandro; Wheatley, R.; Seifert, B.; Wallentowitz, S.; Joshi, T.; Lederman, D.

Highly epitaxial delafossite CuFeO2 and CuFe1-xGaxO2 films were grown using Pulsed Laser Deposition techniques. The sample thicknesses were estimated to be 21 nm, 75 nm.The estimated gallium fraction of substituted ferric atoms was x =0.25 for the composite sample. We present the study of the fundamental band gap(s) for each sample via observation of their respective optical absorption properties in the NIR-VIS region using transmittance and diffuse reflection spectroscopy. Predominant absorption edges measured were between 1.1eV and 3.1eV from transmittance spectra. The sample of CuFe1-xGaxO2 showed measurable absorption features located at 2.4eV and 2.8eV. This study also found evidence of changes between apparent absorption edges between transmittance and diffuse reflectance spectroscopies of each sample and it may be resultant from absorption channels via surface states. Future photoluminescence experiments are planned to determine the photo-induced semiconductor behavior of these materials. ACNOWLEDGEMENTS: This work was supported by FONDECyT 1130372 and Proyecto Anillo ACT1409 at PUC and supported in part by the WV Higher Education Policy Commission (Grant HEPC.dsr.12.29) and by FAME sponsored by MARCO and DARPA (contract # 2013-MA-2382).

Assessment of inflow and washout of indocyanine green in the adult human brain by monitoring of diffuse reflectance at large source-detector separation

NASA Astrophysics Data System (ADS)

Liebert, Adam; Sawosz, Piotr; Milej, Daniel; Kacprzak, Michał; Weigl, Wojciech; Botwicz, Marcin; MaCzewska, Joanna; Fronczewska, Katarzyna; Mayzner-Zawadzka, Ewa; Królicki, Leszek; Maniewski, Roman

2011-04-01

Recently, it was shown in measurements carried out on humans that time-resolved near-infrared reflectometry and fluorescence spectroscopy may allow for discrimination of information originating directly from the brain avoiding influence of contaminating signals related to the perfusion of extracerebral tissues. We report on continuation of these studies, showing that the near-infrared light can be detected noninvasively on the surface of the tissue at large interoptode distance. A multichannel time-resolved optical monitoring system was constructed for measurements of diffuse reflectance in optically turbid medium at very large source-detector separation up to 9 cm. The instrument was applied during intravenous injection of indocyanine green and the distributions of times of flight of photons were successfully acquired showing inflow and washout of the dye in the tissue. Time courses of the statistical moments of distributions of times of flight of photons are presented and compared to the results obtained simultaneously at shorter source-detector separations (3, 4, and 5 cm). We show in a series of experiments carried out on physical phantom and healthy volunteers that the time-resolved data acquisition in combination with very large source-detector separation may allow one to improve depth selectivity of perfusion assessment in the brain.

SeaWiFS Postlaunch Technical Report Series. Volume 5; The SeaWiFS Solar Radiation-Based Calibration and the Transfer-to-Orbit Experiment

NASA Technical Reports Server (NTRS)

Hooker, Stanford B. (Editor); Firestone, Elaine R. (Editor); Barnes, Robert A.; Eplee, Robert E., Jr.; Biggar, Stuart F.; Thome, Kurtis J.; Zalewski, Edward F.; Slater, Philip N.; Holmes, Alan W.

1999-01-01

The solar radiation-based calibration (SRBC) of the Sea-viewing Wide Field-of-view Sensor (SeaWiFS) was performed on 1 November 1993. Measurements were made outdoors in the courtyard of the instrument manufacturer. SeaWiFS viewed the solar irradiance reflected from the sensor's diffuser in the same manner as viewed on orbit. The calibration included measurements using a solar radiometer designed to determine the transmittances of principal atmospheric constituents. The primary uncertainties in the outdoor measurements are the transmission of the atmosphere and the reflectance of the diffuser. Their combined uncertainty is about 5 or 6%. The SRBC also requires knowledge of the extraterrestrial solar spectrum. Four solar models are used. When averaged over the responses of the SeaWiFS bands, the irradiance models agree at the 3.6% level, with the greatest difference for SeaWiFS band 8. The calibration coefficients from the SRBC are lower than those from the laboratory calibration of the instrument in 1997. For a representative solar model, the ratios of the SRBC coefficients to laboratory values average 0.962 with a standard deviation of 0.012. The greatest relative difference is 0.946 for band 8. These values are within the estimated uncertainties of the calibration measurements. For the transfer-to-orbit experiment, the measurements in the manufacturer's courtyard are used to predict the digital counts from the instrument on its first day on orbit (August 1, 1997). This experiment requires an estimate of the relative change in the diffuser response for the period between the launch of the instrument and its first solar measurements on orbit (September 9, 1997). In relative terms, the counts from the instrument on its first day on orbit averaged 1.3% higher than predicted, with a standard deviation of 1.2% and a greatest difference of 2.4% or band 7. The estimated uncertainty for the transfer-to-orbit experiment is about 3 or 4%.

Anomalous Diffusion Measured by a Twice-Refocused Spin Echo Pulse Sequence: Analysis Using Fractional Order Calculus

PubMed Central

2011-01-01

Purpose To theoretically develop and experimentally validate a formulism based on a fractional order calculus (FC) diffusion model to characterize anomalous diffusion in brain tissues measured with a twice-refocused spin-echo (TRSE) pulse sequence. Materials and Methods The FC diffusion model is the fractional order generalization of the Bloch-Torrey equation. Using this model, an analytical expression was derived to describe the diffusion-induced signal attenuation in a TRSE pulse sequence. To experimentally validate this expression, a set of diffusion-weighted (DW) images was acquired at 3 Tesla from healthy human brains using a TRSE sequence with twelve b-values ranging from 0 to 2,600 s/mm2. For comparison, DW images were also acquired using a Stejskal-Tanner diffusion gradient in a single-shot spin-echo echo planar sequence. For both datasets, a Levenberg-Marquardt fitting algorithm was used to extract three parameters: diffusion coefficient D, fractional order derivative in space β, and a spatial parameter μ (in units of μm). Using adjusted R-squared values and standard deviations, D, β and μ values and the goodness-of-fit in three specific regions of interest (ROI) in white matter, gray matter, and cerebrospinal fluid were evaluated for each of the two datasets. In addition, spatially resolved parametric maps were assessed qualitatively. Results The analytical expression for the TRSE sequence, derived from the FC diffusion model, accurately characterized the diffusion-induced signal loss in brain tissues at high b-values. In the selected ROIs, the goodness-of-fit and standard deviations for the TRSE dataset were comparable with the results obtained from the Stejskal-Tanner dataset, demonstrating the robustness of the FC model across multiple data acquisition strategies. Qualitatively, the D, β, and μ maps from the TRSE dataset exhibited fewer artifacts, reflecting the improved immunity to eddy currents. Conclusion The diffusion-induced signal attenuation in a TRSE pulse sequence can be described by an FC diffusion model at high b-values. This model performs equally well for data acquired from the human brain tissues with a TRSE pulse sequence or a conventional Stejskal-Tanner sequence. PMID:21509877

Anomalous diffusion measured by a twice-refocused spin echo pulse sequence: analysis using fractional order calculus.

PubMed

Gao, Qing; Srinivasan, Girish; Magin, Richard L; Zhou, Xiaohong Joe

2011-05-01

To theoretically develop and experimentally validate a formulism based on a fractional order calculus (FC) diffusion model to characterize anomalous diffusion in brain tissues measured with a twice-refocused spin-echo (TRSE) pulse sequence. The FC diffusion model is the fractional order generalization of the Bloch-Torrey equation. Using this model, an analytical expression was derived to describe the diffusion-induced signal attenuation in a TRSE pulse sequence. To experimentally validate this expression, a set of diffusion-weighted (DW) images was acquired at 3 Tesla from healthy human brains using a TRSE sequence with twelve b-values ranging from 0 to 2600 s/mm(2). For comparison, DW images were also acquired using a Stejskal-Tanner diffusion gradient in a single-shot spin-echo echo planar sequence. For both datasets, a Levenberg-Marquardt fitting algorithm was used to extract three parameters: diffusion coefficient D, fractional order derivative in space β, and a spatial parameter μ (in units of μm). Using adjusted R-squared values and standard deviations, D, β, and μ values and the goodness-of-fit in three specific regions of interest (ROIs) in white matter, gray matter, and cerebrospinal fluid, respectively, were evaluated for each of the two datasets. In addition, spatially resolved parametric maps were assessed qualitatively. The analytical expression for the TRSE sequence, derived from the FC diffusion model, accurately characterized the diffusion-induced signal loss in brain tissues at high b-values. In the selected ROIs, the goodness-of-fit and standard deviations for the TRSE dataset were comparable with the results obtained from the Stejskal-Tanner dataset, demonstrating the robustness of the FC model across multiple data acquisition strategies. Qualitatively, the D, β, and μ maps from the TRSE dataset exhibited fewer artifacts, reflecting the improved immunity to eddy currents. The diffusion-induced signal attenuation in a TRSE pulse sequence can be described by an FC diffusion model at high b-values. This model performs equally well for data acquired from the human brain tissues with a TRSE pulse sequence or a conventional Stejskal-Tanner sequence. Copyright © 2011 Wiley-Liss, Inc.

The role of tissue microstructure and water exchange in biophysical modelling of diffusion in white matter.

PubMed

Nilsson, Markus; van Westen, Danielle; Ståhlberg, Freddy; Sundgren, Pia C; Lätt, Jimmy

2013-08-01

Biophysical models that describe the outcome of white matter diffusion MRI experiments have various degrees of complexity. While the simplest models assume equal-sized and parallel axons, more elaborate ones may include distributions of axon diameters and axonal orientation dispersions. These microstructural features can be inferred from diffusion-weighted signal attenuation curves by solving an inverse problem, validated in several Monte Carlo simulation studies. Model development has been paralleled by microscopy studies of the microstructure of excised and fixed nerves, confirming that axon diameter estimates from diffusion measurements agree with those from microscopy. However, results obtained in vivo are less conclusive. For example, the amount of slowly diffusing water is lower than expected, and the diffusion-encoded signal is apparently insensitive to diffusion time variations, contrary to what may be expected. Recent understandings of the resolution limit in diffusion MRI, the rate of water exchange, and the presence of microscopic axonal undulation and axonal orientation dispersions may, however, explain such apparent contradictions. Knowledge of the effects of biophysical mechanisms on water diffusion in tissue can be used to predict the outcome of diffusion tensor imaging (DTI) and of diffusion kurtosis imaging (DKI) studies. Alterations of DTI or DKI parameters found in studies of pathologies such as ischemic stroke can thus be compared with those predicted by modelling. Observations in agreement with the predictions strengthen the credibility of biophysical models; those in disagreement could provide clues of how to improve them. DKI is particularly suited for this purpose; it is performed using higher b-values than DTI, and thus carries more information about the tissue microstructure. The purpose of this review is to provide an update on the current understanding of how various properties of the tissue microstructure and the rate of water exchange between microenvironments are reflected in diffusion MRI measurements. We focus on the use of biophysical models for extracting tissue-specific parameters from data obtained with single PGSE sequences on clinical MRI scanners, but results obtained with animal MRI scanners are also considered. While modelling of white matter is the central theme, experiments on model systems that highlight important aspects of the biophysical models are also reviewed.

Diffuse reflectance spectroscopy for optical nerve identification. Preliminary ex vivo results for feedback controlled oral and maxillofacial laser surgery

NASA Astrophysics Data System (ADS)

Stelzle, Florian; Zam, Azhar; Adler, Werner; Douplik, Alexandre; Tangermann-Gerk, Katja; Nkenke, Emeka; Neukam, Friedrich Wilhelm; Schmidt, Michael

Objective: Laser surgery has many advantages. However, due to a lack of haptic feedback it is accompanied by the risk of iatrogenic nerve damage. The aim of this study was to evaluate the possibilities of optical nerve identification by diffuse reflectance spectroscopy to set the base for a feedback control system to enhance nerve preservation in oral and maxillofacial laser surgery. Materials and Methods: Diffuse reflectance spectra of nerve tissue, skin, mucosa, fat tissue, muscle, cartilage and bone (15120 spectra) of ex vivo pig heads were acquired in the wavelength range of 350-650 nm. Tissue differentiation was performed by principal components analysis (PCA) followed by linear discriminant analysis (LDA). Specificity and sensitivity were calculated by receiver operating characteristic (ROC) analysis and the area under curve (AUC). Results: Nerve tissue could correctly be identified and differed from skin, mucosa, fat tissue, muscle, cartilage and bone in more than 90% of the cases (AUC results) with a specificity of over 78% and a sensitivity of more than 86%. Conclusion: Nerve tissue can be identified by diffuse reflectance spectroscopy with high precision and reliability. The results may set the base for a feedback system to prevent iatrogenic nerve damage performing oral and maxillofacial laser surgery.

On the renormalisation of the diffusion asymptotics in the problem of reflection of a narrow optical beam from a biological medium

DOE Office of Scientific and Technical Information (OSTI.GOV)

Appanov, A Yu; Barabanenkov, Yu N

2005-12-31

An analytic hybrid method is considered for solving the stationary radiation transfer equation in the problem on reflection of a narrow laser beam from biological media such as the 2% aqueous solution of intralipid and erythrocyte suspension with the volume concentration (hematocrit) H=0.41. The method is based on the reciprocity of the Green function in the radiation transfer theory and on the iteration solution of the integral equation for this function. As a result, the ray intensity is represented as a sum of two terms. The first of them describes the contribution of finite-order scattering to the intensity of amore » beam diffusely reflected from the medium. The second term contains the explicit analytic expression for a spatially distributed effective source of diffuse radiation emerging from the deep layers of the medium to the surface. This approach substantially improves the diffusion approximation for the problem under study and allows one to obtain the uniform asymptotics of the reflection coefficient at the specified interval of distances between the radiation source and detector on the medium surface with the relative error within {+-}6% for the 2% intralipid emulsion and erythrocyte suspension (H=0.41). (radiation scattering)« less