Sample records for mesuusataja dario cologna

  1. The Dario Bacas Goniobarimeter: Building a Balance Based on Properties of the Cycloid

    ERIC Educational Resources Information Center

    Pantin, Javier del Rey; de Zarate, Jose M. Ortiz


    In this article we describe and build a model of a historical weighing device proposed by the Spanish engineer Dario Bacas in the second half of the 19th century. The balance was named "goniobarimeter" by its inventor, and the weighing principle is based on a curious, and not very well known, property of the cycloid. The simplicity of the design…

  2. plantDARIO: web based quantitative and qualitative analysis of small RNA-seq data in plants.


    Patra, Deblina; Fasold, Mario; Langenberger, David; Steger, Gerhard; Grosse, Ivo; Stadler, Peter F


    High-throughput sequencing techniques have made it possible to assay an organism's entire repertoire of small non-coding RNAs (ncRNAs) in an efficient and cost-effective manner. The moderate size of small RNA-seq datasets makes it feasible to provide free web services to the research community that provide many basic features of a small RNA-seq analysis, including quality control, read normalization, ncRNA quantification, and the prediction of putative novel ncRNAs. DARIO is one such system that so far has been focussed on animals. Here we introduce an extension of this system to plant short non-coding RNAs (sncRNAs). It includes major modifications to cope with plant-specific sncRNA processing. The current version of plantDARIO covers analyses of mapping files, small RNA-seq quality control, expression analyses of annotated sncRNAs, including the prediction of novel miRNAs and snoRNAs from unknown expressed loci and expression analyses of user-defined loci. At present Arabidopsis thaliana, Beta vulgaris, and Solanum lycopersicum are covered. The web tool links to a plant specific visualization browser to display the read distribution of the analyzed sample. The easy-to-use platform of plantDARIO quantifies RNA expression of annotated sncRNAs from different sncRNA databases together with new sncRNAs, annotated by our group. The plantDARIO website can be accessed at

  3. Prevalence of obesity and associated cardiovascular risk: the DARIOS study

    PubMed Central


    Background To estimate the prevalence of overweight and obesity in the Spanish population as measured with body mass index (BMI), waist circumference (WC) and waist to height ratio (WHtR) and to determine the associated cardiovascular risk factors. Methods Pooled analysis with individual data from 11 studies conducted in the first decade of the 21st century. Participants aged 35–74 years were asked about the history of cardiovascular diseases, hypertension, diabetes and hypercholesterolemia. Height, weight, WC, blood pressure, glycaemia, total cholesterol, low-density and high-density lipoprotein cholesterol and coronary risk were measured. The prevalence of overweight (BMI 25–29.9 kg/m2), general obesity (BMI ≥30 kg/m2), suboptimal WC (≥ 80 cm and < 88 in women, ≥ 94 and < 102 in men), abdominal obesity (WC ≥88 cm ≥102 cm in women and men, respectively) and WHtR ≥0.5 was estimated, standardized for the European population. Results We included 28,743 individuals. The prevalence of overweight and suboptimal WC was 51% and 30% in men and 36% and 22% in women, respectively; general obesity was 28% in both sexes and abdominal obesity 36% in men and 55% in women. The prevalence of WHtR ≥0.5 was 89% and 77% in men and women, respectively. All cardiovascular risk factors were significantly associated with abnormal increased values of BMI, WC and WHtR. Hypertension showed the strongest association with overweight [OR = 1.99 (95% confidence interval 1.81-2.21) and OR = 2.10 (1.91-2.31)]; suboptimal WC [OR = 1.78 (1.60-1.97) and OR = 1.45 (1.26-1.66)], with general obesity [OR = 4.50 (4.02-5.04), and OR = 5.20 (4.70-5.75)] and with WHtR ≥0.5 [OR = 2.94 (2.52-3.43), and OR = 3.02 (2.66-3.42)] in men and women respectively, besides abdominal obesity in men only [OR = 3.51 (3.18-3.88)]. Diabetes showed the strongest association with abdominal obesity in women [OR = 3,86 (3,09-4,89). Conclusions The prevalence of obesity in Spain was high. Overweight, suboptimal WC, general, abdominal obesity and WHtR ≥0.5 was significantly associated with diabetes, hypertension, hypercholesterolemia and coronary risk. The use of lower cut-off points for both BMI and particularly WC and could help to better identify the population at risk and therefore achieve more effective preventive measures. PMID:23738609

  4. Characterization of DYRK2 ( dual-specificity tyrosine-phosphorylation-regulated kinase 2) from Zebrafish ( Dario rerio)

    NASA Astrophysics Data System (ADS)

    Sun, Wei; Tan, Xungang; Zhang, Peijun; Zhang, Yuqing; Xu, Yongli


    Proteins of the DYRK (dual-specificity tyrosine-phosphorylation-regulated kinase) family are characterized by the presence of a conserved kinase domain and N-terminal DH box. DYRK2 is involved in regulating key developmental and cellular processes, such as neurogenesis, cell proliferation, cytokinesis, and cellular differentiation. Herein, we report that the ortholog of DYRK2 found in zebrafish shares about 70% identity with that of human, mouse, and chick. RT-PCR showed that DYRK2 is expressed maternally and zygotically. In-situ hybridization results show that DYRK2 is expressed in somite cells that will develop into muscles. Our results provide preliminary evidence for investigating the in-vivo function of DYRK2 in zebrafish muscle development.

  5. The Author and His Works

    ERIC Educational Resources Information Center

    Spain Today, 1971


    Works of Emilio Garcia Gomez, Dario Fernandez Florez, Armando Lopez Salinas, Jaime de Arminan, Luis Lopez Anglada, and Carmen Bravo Villasante are analyzed in this continuing series on Spanish authors. (DS)

  6. Heparanase Mechanisms in Melanoma Brain Metastasis

    DTIC Science & Technology


    recently reported the HPSE inhibition by microRNA 1258 which resulted in a suppression of brain metastasis in in xenograft models of breast cancer...AWARD NUMBER: W81XWH-12-1-0281 TITLE: Heparanase Mechanisms in Melanoma Brain Metastasis PRINCIPAL INVESTIGATOR: Dr. Dario Marchetti...Mechanisms in Melanoma Brain Metastasis 5b. GRANT NUMBER W81XWH-12-1-0281 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d. PROJECT NUMBER Dario Marchetti

  7. Heparanase Mechanisms in Melanoma Brain Metastasis

    DTIC Science & Technology


    Melanoma Brain Metastasis PRINCIPAL INVESTIGATOR: Dr. Dario Marchetti RECIPIENT: Baylor College of Medicine REPORT DATE...Annual 3. DATES COVERED 1 Aug 2013-31 Jul 2014 4. TITLE AND SUBTITLE 5a. CONTRACT NUMBER Heparanase Mechanisms in Melanoma Brain...brain. This emphasizes the potential for therapeutically targeting this enzyme in brain metastasis in general, brain-metastatic melanoma (BMM) in

  8. Reflective Praxis through Narrative and Poetry: Performing "Peace Mum"

    ERIC Educational Resources Information Center

    Prendergast, Monica


    In the autumn of 2007 and spring of 2008 the author performed an adapted version of Dario Fo and Franca Rame's one-woman play "Peace Mom" (retitled for Canadian audiences as "Peace Mum") about American mother and peace activist Cindy Sheehan. The play was performed for University of Victoria Applied Theatre students and also in a number of…

  9. Mechanisms of CTC Biomarkers in Breast Cancer Brain Metastasis

    DTIC Science & Technology


    AWARD NUMBER: W81XWH-14-1-0214 TITLE: Mechanisms of CTC Biomarkers in Breast Cancer Brain Metastasis PRINCIPAL INVESTIGATOR: Dario...5a. CONTRACT NUMBER Mechanisms of CTC Biomarkers in Breast Cancer Brain Metastasis 5b. GRANT NUMBER W81XWH-14-1-0214 5c. PROGRAM ELEMENT NUMBER 6...SUPPLEMENTARY NOTES None 14. ABSTRACT Breast cancer brain metastasis (BCBM) is devastating and increasing in frequency, however, BCBM mechanisms are

  10. The World Factbook 2002

    DTIC Science & Technology


    recession; a series of reforms to bolster the other: 0.29% (2000) coalition) [Dario Pedro ALESSANDRO ]; Justicialist domestic banking system followed...three principal rivers of the Black, Red, and White T * Dioulasso Voltas conventional long form: none Banfora P I conventional short form: Burkina...Faso P e 0 p I e former: Upper Volta , Republic of Upper Volta GHANA BENIN Population: 12,603,185 Government type: parliamentary republic C TE 4 TOGO note

  11. Performance of the Volumetric Diffusive Respirator at Altitude

    DTIC Science & Technology


    AFRL-SA-WP-SR-2014-0020 Performance of the Volumetric Diffusive Respirator at Altitude Dario Rodriquez, MSc1; Tyler Britton, RRT2...the Volumetric Diffusive Respirator at Altitude 5a. CONTRACT NUMBER FA8650-12-2-6B012 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6...volumetric diffusive respirator is a pneumatic ventilator used by the U.S. Army Burn Team and the U.S. Air Force Lung Team for patients with hypoxemic

  12. A study on the phenomena of flash-sintering with tetragonal zirconia

    NASA Astrophysics Data System (ADS)

    Francis, John Stanley Curtis

    A new method for the sintering of ceramics will be presented in detail. This method called Flash-Sintering was first reported in 2010 by Cologna et al. In Flash-Sintering an electric field is applied across a "green" sample with a pair of electrodes and the sintering is measured as a function of the field and temperature. The electric field is shown to remarkably enhance densification. Both the sintering time to achieve near full density and the temperature required are reduced substantially. These changes allow for sintering of 3m% yttria stabilized zirconia at furnace temperatures below 850°C in a matter of seconds. The objective of this dissertation is to understand the phenomenological behavior of flash-sintering. This new method is a highly non-linear event which occurs at a particular temperature for a given applied field and sintering is accompanied by an abrupt rise in the conductivity. The development of relationships between the electrical control parameters, the sintering behavior, and the evolution of the microstructure are the principal themes of this doctoral research. The present work covers the following topics: (i) The influence of uniaxial pressure applied in combination with electrical field on sintering and superplastic deformation, which show an equivalence between mechanical and electrical driving forces, (ii) A shift of the flash to a higher temperature with increasing particle size of the ceramic powders, (iii) The influence of the electric field on the incubation time for the onset of the flash in experiments carried out at isothermal furnace temperatures, and the effect of the current density immediately following the flash on densification, (iv) The relationship between electrical parameters on microstructure (grain size) evolution, (v) A comparison with the microstructure and mechanical strength of specimens prepared by conventional sintering, and (vi) Measurement of luminescence spectra, which lies in the visible range, that

  13. A study on flash sintering and related phenomena in titania and its composite with alumina

    NASA Astrophysics Data System (ADS)


    In 2010, Cologna et. al. [1] reported that with a help of small electric field 120 Vcm-1, the sintering temperature of 3 mol % yittria stabilized zirconia could be brought down to 850°C from 1450°C. On top of reducing the temperature requirements, the green sample could be sintered from starting density of 50% to near full density in mere 5 seconds, a sintering rate three orders of magnitude higher than conventional methods. This discovery led to the emergence of a new field of enhanced sintering with electric field, named "Flash Sintering". The objective of this thesis is to understand the phenomenological behavior of flash-sintering and related phenomena on titania and its composites with alumina at elevated temperature. The possible mechanisms to explain flash sintering are discussed: Joule heating and the avalanche of defect generation [2], both induced by the rapid rise in conductivity just before the onset of the flash. Apparently, both mechanisms play a role. The thesis covers the response of pure titania and composites of titania-alumina under flash and compared with conventional sintering. We start with the sintering behavior of pure titania and observe lowering of sintering temperature requirements with higher applied electric field. The conductivity of titania during flash is also measured, and compared with the nominal conductivity of titania at equivalent temperatures. The conductivity during flash is determined to be have a different activation energy. For the composites of titania-alumina, effect of flash on the constrained sintering was studied. It is a known fact that sintering of one component of composite slows down when the other component of a different densification rate is added to it, called constrained sintering. In our case, large inclusions of alumina particles were added to nano-grained titania green compact that hindered its densification. Flash sintering was found to be overcoming this problem and near full densification was achieved

  14. GMAG Dissertation Award Talk: Zero-moment Half-Metallic Ferrimagnetic Semiconductors

    NASA Astrophysics Data System (ADS)

    Jamer, Michelle E.


    Low- and zero-moment half-metallic ferrimagnetic semiconductors have been proposed for advanced applications, such as nonvolatile RAM memory and quantum computing. These inverse-Heusler materials could be used to generate spin-polarized electron or hole currents without the associated harmful fringing magnetic fields. Such materials are expected to exhibit low to zero magnetic moment at room temperature, which makes them well-positioned for future spin-based devices. However, these compounds have been shown to suffer from disorder. This work focuses on the synthesis of these compounds and the investigation of their structural, magnetic, and transport properties. Cr2CoGa and Mn3Al thin films were synthesized by molecular beam epitaxy, and V3Al and Cr2CoAl were synthesized via arc-melting. Rietveld analysis was used to determine the degree of ordering in the sublattices as a function of annealing. The atomic moments were measured by X-ray magnetic circular and linear dichroism confirmed antiferromagnetic alignment of sublattices and the desired near-zero moment in several compounds. In collaboration with George E. Sterbinsky, Photon Sciences Directorate, Brookhaven National Laboratory; Dario Arena Photon Sciences Directorate, Brookhaven National Laboratory; Laura H. Lewis, Chemical Engineering, Northeastern University; and Don Heiman, Physics, Northeastern University. NSF-ECCS-1402738, NSF-DMR-0907007.

  15. Thermoelectrics from silicon nanoparticles: the influence of native oxide

    NASA Astrophysics Data System (ADS)

    Petermann, Nils; Stötzel, Julia; Stein, Niklas; Kessler, Victor; Wiggers, Hartmut; Theissmann, Ralf; Schierning, Gabi; Schmechel, Roland


    Thermoelectric materials were synthesized by current-assisted sintering of doped silicon nanoparticles produced in a microwave-plasma reactor. Due to their affinity to oxygen, the nanoparticles start to oxidize when handled in air and even a thin surface layer of native silicon oxide leads to a significant increase in the oxide volume ratio. This results in a considerable incorporation of oxygen into the sintered pellets, thus affecting the thermoelectric performance. To investigate the necessity of inert handling of the raw materials, the thermoelectric transport properties of sintered nanocrystalline silicon samples were characterized with respect to their oxygen content. An innovative method allowing a quantitative silicon oxide analysis by means of electron microscopy was applied: the contrast between areas of high and low electrical conductivity was attributed to the silicon matrix and silicon oxide precipitates, respectively. Thermoelectric characterization revealed that both, electron mobility and thermal conductivity decrease with increasing silicon oxide content. A maximum figure of merit with zT = 0.45 at 950 °C was achieved for samples with a silicon oxide mass fraction of 9.5 and 21.4% while the sample with more than 25% of oxygen clearly indicates a negative impact of the oxygen on the electron mobility. Contribution to the Topical Issue "Silicon and Silicon-related Materials for Thermoelectricity", edited by Dario Narducci.

  16. Your Brain on Art: Emergent Cortical Dynamics During Aesthetic Experiences

    PubMed Central

    Kontson, Kimberly L.; Megjhani, Murad; Brantley, Justin A.; Cruz-Garza, Jesus G.; Nakagome, Sho; Robleto, Dario; White, Michelle; Civillico, Eugene; Contreras-Vidal, Jose L.


    The brain response to conceptual art was studied with mobile electroencephalography (EEG) to examine the neural basis of aesthetic experiences. In contrast to most studies of perceptual phenomena, participants were moving and thinking freely as they viewed the exhibit The Boundary of Life is Quietly Crossed by Dario Robleto at the Menil Collection-Houston. The brain activity of over 400 subjects was recorded using dry-electrode and one reference gel-based EEG systems over a period of 3 months. Here, we report initial findings based on the reference system. EEG segments corresponding to each art piece were grouped into one of three classes (complex, moderate, and baseline) based on analysis of a digital image of each piece. Time, frequency, and wavelet features extracted from EEG were used to classify patterns associated with viewing art, and ranked based on their relevance for classification. The maximum classification accuracy was 55% (chance = 33%) with delta and gamma features the most relevant for classification. Functional analysis revealed a significant increase in connection strength in localized brain networks while subjects viewed the most aesthetically pleasing art compared to viewing a blank wall. The direction of signal flow showed early recruitment of broad posterior areas followed by focal anterior activation. Significant differences in the strength of connections were also observed across age and gender. This work provides evidence that EEG, deployed on freely behaving subjects, can detect selective signal flow in neural networks, identify significant differences between subject groups, and report with greater-than-chance accuracy the complexity of a subject's visual percept of aesthetically pleasing art. Our approach, which allows acquisition of neural activity “in action and context,” could lead to understanding of how the brain integrates sensory input and its ongoing internal state to produce the phenomenon which we term aesthetic experience

  17. Cold plasma treatment in wound care: efficacy and risk assessment

    NASA Astrophysics Data System (ADS)

    Stoffels, Eva


    Cold atmospheric plasma is an ideal medium for non-destructive modification of vulnerable surfaces. One of the most promising medical applications of cold plasma treatment is wound healing. Potential advantages in wound healing have been demonstrated in vitro: the plasma does not necrotize the cells and does not affect the extracellular matrix [1], has clear bactericidal or bacteriostatic effects [2], and stimulates fibroblast cells towards faster attachment and proliferation [3]. However, safety issues, such as the potential cytotoxicity of the plasma must be clarified prior to clinical implementation. This work comprises the recent facts on sub-lethal plasma effects on mammalian cells, as well as studies on apoptosis induction and quantitative assessment of DNA damage. Fibroblast, smooth muscle and endothelial cells were treated using the standard cold plasma needle [1,2]; intra- and extracellular oxidant levels as well as the influence of the plasma on intracellular antioxidant balance were monitored using appropriate fluorescent markers [1]. We have studied long-term cellular damage was monitored using flow cytometry to determine the DNA profiles in treated cells. Dose-response curves were obtained: increased proliferation as well as apoptosis were visualized under different treatment conditions. The results from the in vitro studies are satisfying. [1] I.E. Kieft, ``Plasma needle: exploring biomedical applications of non-thermal plasmas'', PhD Thesis, Eindhoven University of Technology (2005). [2] R.E.J. Sladek, ``Plasma needle: non-thermal atmospheric plasmas in dentistry'' PhD Thesis, Eindhoven University of Technology (2006). [3] I.E. Kieft, D. Darios, A.J.M. Roks, E. Stoffels, IEEE Trans. Plasma Sci. 34(4), 2006, pp. 1331-1336.

  18. Plastic matters: an analytical procedure to evaluate the degradability of contemporary works of art.


    Lazzari, Massimo; Ledo-Suárez, Ana; López, Thaïs; Scalarone, Dominique; López-Quintela, M Arturo


    The most significant results concerning a chemical study to evaluate the degradability of polymeric components in four contemporary works of art, partially or completely realized in plastics, are presented and discussed in this paper. The procedure applied is mainly based on the use of Fourier transform IR and UV-vis spectroscopies and pyrolysis-gas chromatography/mass spectrometry, and consists of the following steps: (1) compositional analysis of the artworks, with particular attention to components which may have a negative effect on the overall ageing; (2) evaluation of the actual state of conservation; (3) investigation of the accelerated ageing of reference polymer samples; and (4) monitoring of the natural ageing of the artworks. On such a basis, the following could be concluded. Stage Evidence by Loris Cecchini is made of poly(ether urethane) elastomer which contains a high amount of phthalates. Their exudation gives a sticky appearance to the artwork and their removal during ageing is the main cause of the loss of flexibility. The latex used by Andrés Pinal for tailoring Traxe de Home is a natural polyisoprene, whose oxidative degradation accounts for the extensive deterioration and yellowing of the artwork. The plaster sculptures of 3D Bodyscans 1:9 by Karin Sander are coated with an aliphatic epoxy resin. Its oxidation with formation of amides is the cause of the surface yellowing. The adhesive used by Dario Villalba for Tierra, Ladrillo y Agua is a commercial poly(vinyl acetate). Simulated photoageing suggests a fast deterioration due to deacetylation and cross-linking, which possibly is the main reason for the actual detachment of debris from the support.

  19. IBC's 23rd Antibody Engineering and 10th Antibody Therapeutics Conferences and the Annual Meeting of The Antibody Society: December 2-6, 2012, San Diego, CA.


    Marquardt, John; Begent, Richard H J; Chester, Kerry; Huston, James S; Bradbury, Andrew; Scott, Jamie K; Thorpe, Philip E; Veldman, Trudi; Reichert, Janice M; Weiner, Louis M


    Now in its 23rd and 10th years, respectively, the Antibody Engineering and Antibody Therapeutics conferences are the Annual Meeting of The Antibody Society. The scientific program covers the full spectrum of challenges in antibody research and development from basic science through clinical development. In this preview of the conferences, the chairs provide their thoughts on sessions that will allow participants to track emerging trends in (1) the development of next-generation immunomodulatory antibodies; (2) the complexity of the environment in which antibodies must function; (3) antibody-targeted central nervous system (CNS) therapies that cross the blood brain barrier; (4) the extension of antibody half-life for improved efficacy and pharmacokinetics (PK)/pharmacodynamics (PD); and (5) the application of next generation DNA sequencing to accelerate antibody research. A pre-conference workshop on Sunday, December 2, 2012 will update participants on recent intellectual property (IP) law changes that affect antibody research, including biosimilar legislation, the America Invents Act and recent court cases. Keynote presentations will be given by Andreas Plückthun (University of Zürich), who will speak on engineering receptor ligands with powerful cellular responses; Gregory Friberg (Amgen Inc.), who will provide clinical updates of bispecific antibodies; James D. Marks (University of California, San Francisco), who will discuss a systems approach to generating tumor targeting antibodies; Dario Neri (Swiss Federal Institute of Technology Zürich), who will speak about delivering immune modulators at the sites of disease; William M. Pardridge (University of California, Los Angeles), who will discuss delivery across the blood-brain barrier; and Peter Senter (Seattle Genetics, Inc.), who will present his vision for the future of antibody-drug conjugates. For more information on these meetings or to register to attend, please visit www

  20. A 500-year overview and analysis of flood changes in Europe: preliminary results

    NASA Astrophysics Data System (ADS)

    Kiss, Andrea


    Long-term flood series can be gained by combining evidence and systematic hydrological observations. Following various already existing local and regional studies, an important aim of the present work is to create a broad European database of long flood chronologies and to use them for detecting changes in flood regimes with respect to common break points. Another aim of the investigations is to reveal the main causes (e.g. atmospheric, human) of these changes and study spatial and temporal variability of floods on a European scale. In the presentation we provide an overview on the current stage of these Europe-wide investigations, including the available source types (i.e. documentary and instrumental), geographical coverage, temporal and spatial distribution of long-term flood series applied in the study. The first research results concerns basic information on magnitude, frequency and seasonality of floods (with special consideration of detectable changes). Full list of authors in alphabetic order: Mariano Barriendos (1), Günter Blöschl (2), Rudolf Brázdil (3), Gerardo Benito (4), Chiara Bertolin (5), Dario Camuffo (5), Gaston Demarée (6), Líbor Elleder (7), Silvi Enzi (8), Rüdiger Glaser (9), Julia Hall (2), Andrea Kiss (2), Oldrich Kotyza (10), Carmen Maria del Llasat (1), Neil MacDonald (11), Rui Perdigao (2), Dag Retsö (12), Lars Roald (13), Josep Luis Ruiz Bellet (1), Johannes Schönbeim (9), Petra Schmocker-Fackel (14), Lothar Schulte (1), Hubert Valasek (15), Oliver Wetter (16) (1) Faculty of Geography and History, University of Barcelona, Spain (2) Institute of Hydrological Engineering and Water Resources Management, TU Wien (3) Institute of Geography, Masaryk University Brno, Czech Republic (4) Laboratory of Hydrology and Geomorphology, Center of Env. Sciences, Madrid, Spain (5) Institute of Atmospheric Sciences and Climate, National Research Council, Rome, Italy (6) Royal Meteorological Institute, Brussels, Belgium (7) Research Group of

  1. Expression of the T85A mutant of zebrafish aquaporin 3b improves post-thaw survival of cryopreserved early mammalian embryos.


    Bedford-Guaus, Sylvia J; Chauvigné, François; Mejía-Ramírez, Eva; Martí, Mercè; Ventura-Rubio, Antoni; Raya, Ángel; Cerdà, Joan; Veiga, Anna


    While vitrification has become the method of choice for preservation of human oocytes and embryos, cryopreservation of complex tissues and of large yolk-containing cells, remains largely unsuccessful. One critical step in such instances is appropriate permeation while avoiding potentially toxic concentrations of cryoprotectants. Permeation of water and small non-charged solutes, such as those used as cryoprotectants, occurs largely through membrane channel proteins termed aquaporins (AQPs). Substitution of a Thr by an Ala residue in the pore-forming motif of the zebrafish (Dario rerio) Aqp3b paralog resulted in a mutant (DrAqp3b-T85A) that when expressed in Xenopus or porcine oocytes increased their permeability to ethylene glycol at pH 7.5 and 8.5. The main objective of this study was to test whether ectopic expression of DrAqp3b-T85A also conferred higher resistance to cryoinjury. For this, DrAqp3b-T85A + eGFP (reporter) cRNA, or eGFP cRNA alone, was microinjected into in vivo fertilized 1-cell mouse zygotes. Following culture to the 2-cell stage, appropriate membrane expression of DrAqp3b-T85A was confirmed by immunofluorescence microscopy using a primary specific antibody directed against the C-terminus of DrAqp3b. Microinjected 2-cell embryos were then cryopreserved using a fast-freezing rate and low concentration (1.5 M) of ethylene glycol in order to highlight any benefits from DrAqp3b-T85A expression. Notably, post-thaw survival rates were higher (P<0.05) for T85A-eGFP-injected than for -uninjected or eGFP-injected embryos (73±7.3 vs. 28±7.3 or 14±6.7, respectively). We propose that ectopic expression of mutant AQPs may provide an avenue to improve cryopreservation results of large cells and tissues in which current vitrification protocols yield low survival.

  2. VizieR Online Data Catalog: The Initial Gaia Source List (IGSL) (Smart, 2013)

    NASA Astrophysics Data System (ADS)

    Smart, R. L.; Nicastro, L.


    The IGSL is a compilation catalog produced for the Gaia mission. We have combined data from the following catalogs or datasets to produce a homogenous list of positons, proper motions, photometry in a blue and red band and estimates of the magnitudes in the Gaia G and G_RVS bands. Included Catalogs: Tycho2, LQRF, UCAC4, SDSS-DR9, PPMXL, GSC23, GEPC, OGLE, Sky2000, 2MASS. Note that in compiling the various entries we did not consider the individual flags. Overall, we think this catalog is reliable but there will be errors, mismatches and duplicates. The user should use this catalog with that in mind, it is fine for statistical studies that has some way to remove obviously incorrect entries but it should only be used with care for individual objects. The source catalogs used to produce the IGSL are: * The Gaia Ecliptic Pole Catalog, version 3.0 (GEPC) Altmann & Bastian 2009, "Ecliptic Poles Catalogue Version 1.1" ESA Document GAIA-C3-TN-ARI-MA-002 URL * GSC2.3: GSC2 version 2.3, Lasker et al. 2008AJ....136..735L (I/305) * an excerpt of the 4th version of the Gaia Initial QSO Catalog (GIQC) as compiled by the GWP-S-335-13000, formed by Alexandre H. Andrei, Christophe Barache, Dario N. da Silva Neto, Francois Taris, Geraldine Bourda, Jean-Francois Le Campion, Jean Souchay, J.J. Pereira Osorio, Julio I. Bueno de Camargo, Marcelo Assafin, Roberto Vieira Martins, Sebastien Bouquillon, Sebastien Lambert, Sonia Anton, Patrick Charlot * OGLE: Optical Gravitational Lensing Experiment version III (Szymaski et al., 2011, Cat. J/AcA/61/83) * PPMXL: Positions and Proper Motions "Extra Large" Catalog, Roeser et al. (2010, Cat. I/317) * SDSS: Sloan Digital Sky Survey data release 9, Cat. V/139 * UCAC4: Zacharias et al., 2012, Cat. I/322 * Tycho-2, Hoeg et al., 2000, Cat. I/259 (1 data file).

  3. Does canopy mean N concentration explain differences in light use efficiency in 14 eddy-covariance sites?

    NASA Astrophysics Data System (ADS)

    Peltoniemi, Mikko; Pulkkinen, Minna; Kolari, Pasi; Mäkelä, Annikki


    . Mean growing season VPD was the only climatic variable which correlated significantly with the largest actual LUE; none of them correlated with potential LUE. Inclusion of nitrogen in the Prelued-model structure did not improve the goodness of fit of the model. According to our results LUE correlates with mean canopy N concentration. The correlation of mean VPD with the largest actual LUE can also be explained with the model accounting for daily variation in climate, as was made with Prelued-model for the potential LUE. Further studies utilising seasonal values of canopy N are called upon. *Acknowledgements: Eero Nikinmaa, Pertti Hari, Timo Vesala, Tuomas Laurila, Fredrik Lagergren, Meelis Mölder, Anders Lindroth, Thomas Grünwald, Christian Bernhofer, Denis Loustau, Paul Berbigier, Beverly Law, Alison Dunn, Steve Wofsy, Torbjörn Johansson, Torben Christensen, Terry Callaghan, Hans Verbeeck, Remko Duursma, Leonardo Montagnani, Dario Papale, Andreas Ibrom, Ebba Dellwik, Kim Pilegaard, Kentaro Takagi, Eva van Gorsel, Heather Keith, Sonia Wharton, Matthias Falk, Kya Tha Paw U, Matt Schroeder, Jon Lloyd

  4. EDITORIAL: Focus on Dark Matter and Particle Physics

    NASA Astrophysics Data System (ADS)

    Aprile, Elena; Profumo, Stefano


    Doetinchem, H Gast, T Kirn and S Schael Axion searches with helioscopes and astrophysical signatures for axion(-like) particles K Zioutas, M Tsagri, Y Semertzidis, T Papaevangelou, T Dafni and V Anastassopoulos The indirect search for dark matter with IceCube Francis Halzen and Dan Hooper DIRECT DARK MATTER SEARCHES:EXPERIMENTS Gaseous dark matter detectors G Sciolla and C J Martoff Search for dark matter with CRESST Rafael F Lang and Wolfgang Seidel DIRECT AND INDIRECT PARTICLE DARK MATTER SEARCHES:THEORY Dark matter annihilation around intermediate mass black holes: an update Gianfranco Bertone, Mattia Fornasa, Marco Taoso and Andrew R Zentner Update on the direct detection of dark matter in MSSM models with non-universal Higgs masses John Ellis, Keith A Olive and Pearl Sandick Dark stars: a new study of the first stars in the Universe Katherine Freese, Peter Bodenheimer, Paolo Gondolo and Douglas Spolyar Determining the mass of dark matter particles with direct detection experiments Chung-Lin Shan The detection of subsolar mass dark matter halos Savvas M Koushiappas Neutrino coherent scattering rates at direct dark matter detectors Louis E Strigari Gamma rays from dark matter annihilation in the central region of the Galaxy Pasquale Dario Serpico and Dan Hooper DARK MATTER MODELS The dark matter interpretation of the 511 keV line Céline Boehm Axions as dark matter particles Leanne D Duffy and Karl van Bibber Sterile neutrinos Alexander Kusenko Dark matter candidates Lars Bergström Minimal dark matter: model and results Marco Cirelli and Alessandro Strumia Shedding light on the dark sector with direct WIMP production Partha Konar, Kyoungchul Kong, Konstantin T Matchev and Maxim Perelstein Axinos as dark matter particles Laura Covi and Jihn E Kim

  5. PREFACE: Loops 11: Non-Perturbative / Background Independent Quantum Gravity

    NASA Astrophysics Data System (ADS)

    Mena Marugán, Guillermo A.; Barbero G, J. Fernando; Garay, Luis J.; Villaseñor, Eduardo J. S.; Olmedo, Javier


    only was it a showroom for the research currently being carried out by many groups throughout the world, but there was also a permanent look towards the future. During these days, the CSIC Campus witnessed many scientific conversations triggered by the interaction amongst the people and groups that participated in LOOPS'11 Madrid and which, in many cases, will crystallise into new results and advances in the field. The conference would not have been possible without the generous help of a number of national and international institutions. The organizers would like to acknowledge the financial support provided by the Spanish Ministry of Science and Innovation (Ministerio de Ciencia e Innovación), the Spanish Research Council, CSIC (Consejo Superior de Investigaciones Cientĺficas), The BBVA Foundation (Fundación BBVA), The CONSOLIDER-CPAN project, the Spanish Society for Gravitation and Relativity (SEGRE), The Universidad Carlos III of Madrid (UC3M), and the European Science Foundation (ESF). The ESF, through the Quantum Gravity and Quantum Geometry network, provided full support for a number of young participants that have contributed to these proceedings: Dario Benedetti (Albert Einstein Institute, Potsdam, Germany), Norbert Bodendorfer (Institute for Theoretical Physics III, FAU Erlangen Nürnberg, Germany), Mariam Bouhmadi López (CENTRA, Centro Multidisciplinar de Astrofĺsica, Lisbon), Timothy Budd (Institute for Theoretical Physics, Utrecht University, The Netherlands), Miguel Campiglia (Institute for Gravitation and the Cosmos, Penn State University, USA), Gianluca Delfino (School of Mathematical Sciences, University of Nottingham, UK), Maite Dupuis (Institute for Theoretical Physics III, FAU Erlangen Nürnberg, Germany), Michał Dziendzikowski (Institute of Theoretical Physics, Warsaw University, Poland), Muxin Han (Centre de Physique Théorique de Luminy, Marseille, France), Philipp Höhn (Institute for Theoretical Physics, Utrecht University, The

  6. PREFACE: International Conference on Computing in High Energy and Nuclear Physics (CHEP 2010)

    NASA Astrophysics Data System (ADS)

    Lin, Simon C.; Shen, Stella; Neufeld, Niko; Gutsche, Oliver; Cattaneo, Marco; Fisk, Ian; Panzer-Steindel, Bernd; Di Meglio, Alberto; Lokajicek, Milos


    , as well as two banquets held at the Grand Hotel and Grand Formosa Regent in Taipei. The next CHEP conference will be held in New York, the United States on 21-25 May 2012. We would like to thank the National Science Council of Taiwan, the EU ACEOLE project, commercial sponsors, and the International Advisory Committee and the Programme Committee members for all their support and help. Special thanks to the Programme Committee members for their careful choice of conference contributions and enormous effort in reviewing and editing about 340 post conference proceedings papers. Simon C Lin CHEP 2010 Conference Chair and Proceedings Editor Taipei, Taiwan November 2011 Track Editors/ Programme Committee Chair Simon C Lin, Academia Sinica, Taiwan Online Computing Track Y H Chang, National Central University, Taiwan Harry Cheung, Fermilab, USA Niko Neufeld, CERN, Switzerland Event Processing Track Fabio Cossutti, INFN Trieste, Italy Oliver Gutsche, Fermilab, USA Ryosuke Itoh, KEK, Japan Software Engineering, Data Stores, and Databases Track Marco Cattaneo, CERN, Switzerland Gang Chen, Chinese Academy of Sciences, China Stefan Roiser, CERN, Switzerland Distributed Processing and Analysis Track Kai-Feng Chen, National Taiwan University, Taiwan Ulrik Egede, Imperial College London, UK Ian Fisk, Fermilab, USA Fons Rademakers, CERN, Switzerland Torre Wenaus, BNL, USA Computing Fabrics and Networking Technologies Track Harvey Newman, Caltech, USA Bernd Panzer-Steindel, CERN, Switzerland Antonio Wong, BNL, USA Ian Fisk, Fermilab, USA Niko Neufeld, CERN, Switzerland Grid and Cloud Middleware Track Alberto Di Meglio, CERN, Switzerland Markus Schulz, CERN, Switzerland Collaborative Tools Track Joao Correia Fernandes, CERN, Switzerland Philippe Galvez, Caltech, USA Milos Lokajicek, FZU Prague, Czech Republic International Advisory Committee Chair: Simon C. Lin , Academia Sinica, Taiwan Members: Mohammad Al-Turany , FAIR, Germany Sunanda Banerjee, Fermilab, USA Dario Barberis, CERN

  7. PREFACE: Workshop Photograph and Program

    NASA Astrophysics Data System (ADS)


    : Bonnie Fleming (Yale U.))18:30 Supernovae neutrino detection (20') Ines Gil-Botella (CIEMAT)18:50 Neutrino cross-section in Liquid Argon in the GeV range (15') Flavio Cavanna (U. of L'Aquila)19:05 Analysis of the ArgoNeuT neutrino data (15') Carl Bromberg (Michigan State U.)19:20 Neutrino event reconstruction (15') Gary Barker (U. of Warwick) Tuesday 30 March 2010Ways to improve the Liquid Argon Charge Imaging technology I (Chair: Christos Touramanis (U. of Liverpool))09:00 Liquid Argon LEM TPC (30') Filippo Resnati (ETH Zurich)09:30 Micromegas for charge readout of double phase liquid Argon large TPCs (20') Alain Delbart (Saclay)09:50 Development of Thick-GEMs for GEM-TPC Tracker (20') Fuminori Sakuma (RIKEN)10:10 Optical readout of the ionization (20') Neil Spooner (U. of Sheffield)10:30 Scintillation light readout (20') Kostas Mavrokoridis (U. of Liverpool)10:50-11:10 Coffee break Ways to improve the Liquid Argon Charge Imaging technology II (Chair: Alberto Marchionni (ETH Zurich))11:10 Development of cold electronics (30') Veljko Radeka (BNL)11:40 Development of a frontend ASIC and DAQ system Dario Autiero (IPN Lyon)12:00 CAEN digitizers (20') Carlo Tintori (CAEN)12:20 Recent results from Liquid Argon R&D activity (20') Masashi Tanaka (KEK)12:40 Results from the materials test stand and status of LAPD (20') Brian Rebel (FNAL)13:00 Purging and purification: 6 m3 @CERN (20') Alessandro Curioni (ETH Zurich)13:20-14:30 Lunch break14:30-20:00 Trip to J-PARC to visit T2K Beam Facility and Near Detector20:00-22:00 Workshop dinner at Okura Frontier Hotel Tsukuba Wednesday 31 March 2010Ways to improve the Liquid Argon Charge Imaging technology III (Chair: Takasumi Maruyama (KEK))09:00 ArgonTube and UV laser ionization (25') Biagio Rossi (U. of Bern)09:25 Detector magnetization (15') Andreas Badertscher (ETH Zurich)09:40 HV system (25') Sosuke Horikawa (ETH Zurich) Localization studies (Chair: Takuya Hasegawa (KEK))10:05 Okinoshima site study (20') Masakazu Yoshioka (KEK)10

  8. Sensing at the nanoscale

    NASA Astrophysics Data System (ADS)

    Demming, Anna; Hierold, Christofer


    The merits of nanostructures in sensing may seem obvious, yet playing these attributes to their maximum advantage can be a work of genius. As fast as sensing technology is improving, expectations are growing, with demands for cheaper devices with higher sensitivities and an ever increasing range of functionalities and compatibilities. At the same time tough scientific challenges like low power operation, noise and low selectivity are keeping researchers busy. This special issue on sensing at the nanoscale with guest editor Christofer Hierold from ETH Zurich features some of the latest developments in sensing research pushing at the limits of current capabilities. Cheap and easy fabrication is a top priority. Among the most popular nanomaterials in sensing are ZnO nanowires and in this issue Dario Zappa and colleagues at Brescia University in Italy simplify an already cheap and efficient synthesis method, demonstrating ZnO nanowire fabrication directly onto silicon substrates [1]. Meanwhile Nicolae Barson and colleagues in Germany point out the advantages of flame spray pyrolysis fabrication in a topical review [2] and, maximizing on existing resources, researchers in Denmark and Taiwan report cantilever sensing using a US20 commercial DVD-ROM optical pickup unit as the readout source [3]. The sensor is designed to detect physiological concentrations of soluble urokinase plasminogen activator receptor, a protein associated with inflammation due to HIV, cancer and other infectious diseases. With their extreme properties carbon nanostructures feature prominently in the issue, including the demonstration of a versatile and flexible carbon nanotube strain sensor [4] and a graphene charge sensor with sensitivities of the order of 1.3 × 10-3 e Hz-1/2 [5]. The issue of patterning for sensing devices is also tackled by researchers in the US who demonstrate a novel approach for multicomponent pattering metal/metal oxide nanoparticles on graphene [6]. Changes in electrical

  9. PREFACE New developments in nanopore research—from fundamentals to applications New developments in nanopore research—from fundamentals to applications

    NASA Astrophysics Data System (ADS)

    Albrecht, Tim; Edel, Joshua B.; Winterhalter, Mathias


    , Di Cao and Stuart Lindsay Probing DNA with micro- and nanocapillaries and optical tweezers L J Steinbock, O Otto, D R Skarstam, S Jahn, C Chimerel, J L Gornall and U F Keyser Fabrication of nanopores with embedded annular electrodes and transverse carbon nanotube electrodes Zhijun Jiang, Mirna Mihovilovic, Jason Chan and Derek Stein Fabrication and electrical characterization of a pore-cavity-pore device D Pedone, M Langecker, A M Münzer, R Wei, R D Nagel and U Rant Use of tunable nanopore blockade rates to investigate colloidal dispersions G R Willmott, R Vogel, S S C Yu, L G Groenewegen, G S Roberts, D Kozak, W Anderson and M Trau Facilitated translocation of polypeptides through a single nanopore Robert Bikwemu, Aaron J Wolfe, Xiangjun Xing and Liviu Movileanu Mechanistic insight into gramicidin-based detection of protein-ligand interactions via sensitized photoinactivation Tatyana I Rokitskaya, Michael X Macrae, Steven Blake, Natalya S Egorova, Elena A Kotova, Jerry Yang and Yuri N Antonenko Sequence-dependent unfolding kinetics of DNA hairpins studied by nanopore force spectroscopy Stephan Renner, Andrey Bessonov, Ulrich Gerland and Friedrich C Simmel Hydration properties of mechanosensitive channel pores define the energetics of gating A Anishkin, B Akitake, K Kamaraju, C-S Chiang and S Sukharev Dynamic translocation of ligand-complexed DNA through solid-state nanopores with optical tweezers Andy Sischka, Andre Spiering, Maryam Khaksar, Miriam Laxa, Janine König, Karl-Josef Dietz and Dario Anselmetti Force fluctuations assist nanopore unzipping of DNA V Viasnoff, N Chiaruttini, J Muzard and U Bockelmann Control and reversal of the electrophoretic force on DNA in a charged nanopore Binquan Luan and Aleksei Aksimentiev The properties of the outer membrane localized Lipid A transporter LptD Raimund Haarmann, Mohamed Ibrahim, Mara Stevanovic, Rolf Bredemeier and Enrico Schleiff Structural and dynamical properties of the porins OmpF and OmpC: insights from

  10. PREFACE: First Mediterranean Conference on Classical and Quantum Gravity (MCCQG 2009)

    NASA Astrophysics Data System (ADS)

    Basilakos, Spyros; Cadoni, Mariano; Cavaglia, Marco; Christodoulakis, Theodosios; Vagenas, Elias C.


    Fisica, Universidade de Sao Paulo, Brasil) Antoniadis, Ignatios (CERN, Geneva, Switzerland) Arminjon, Mayeul (CNRS, Section of Theoretical Physics, France) Banados, Max (University of Oxford, UK) Basilakos, Spyros (RCAAM, Academy of Athens, Greece) Bastos, Catarina (IST, Departamento de Física, Portugal) Bertolami, Orfeu (IST, Departamento de Física, Portugal) Bevilaqua, Leandro Ibiapina (Instituto de Fisica, Universidade de Sao Paulo, Brazil) Bezerra De Mello, Eugenio (Dept. de Física, CCEN Universidade Federal da Paraíba, Brazil) Blake, Russ (Readify Pty Ltd, Australia) Bogdanos, Charalampos (LPT-Orsay, France) Burinskii, Alexander (Gravity Research Group NSI, Russian Academy of Sciences, Russia) Cadonati, Laura (University of Massachusetts, Amherst, USA) Cadoni, Mariano (Università di Cagliari, Italy) Capone, Monica (University of Turin, Italy) Cavaglià, Marco (University of Mississippi, USA) Chirco, Goffredo (SISSA-International School for Advanced Studies, Italy) Christodoulakis, Theodosios (University of Athens, Greece) Domingues Zarro, Carlos Augusto ((IST, Departamento de Física, Portugal) Durrer, Ruth (Université de Genève, Département de Physique Théorique, Switzerland) Fagnocchi, Serena (SISSA-International School for Advanced Studies, Italy) Finazzi, Stefano (SISSA-International School for Advanced Studies, Italy) Francia, Dario (University Paris 7 - APC, France) Ghosh, Subir (Indian Statistical Institute, India) Gomberoff, Andres (Universidad Catolica de Chile, Chile) Grumiller, Daniel (Institute for Theoretical Physics Vienna University of Technology, Austria) Herrera-Aguilar, Alfredo (IFM, Universidad Michoacana de San Nicolas de Hidalgo, Mexico) Hsu, Steve (University of Oregon, USA) Ichinose, Shoichi (University of Shizuoka, SFNS, Japan) Kiefer, Claus (Institute for Theoretical Physics, University of Cologne, Germany) Kokkotas, Kostas (Theoretical Astrophysics, Eberhard Karls University of Tuebingen, Germany) Kothawala, Dawood (IUCAA, Pune

  11. PREFACE: Fourth Meeting on Constrained Dynamics and Quantum Gravity

    NASA Astrophysics Data System (ADS)

    Cadoni, Mariano; Cavaglia, Marco; Nelson, Jeanette E.


    Cagliari, Italy) Roberto De Pietri (Università di Parma, Italy) Giuseppe De Risi (Università di Bari, Italy) Hans-Thomas Elze (Univ. Federal do Rio de Janeiro, Brasil) Alessandro Fabbri (Università di Bologna, Italy) Sergey Fadeev (VNIIMS, Moscow, Russia) Serena Fagnocchi (Università di Bologna, Italy) Sara Farese (Universidad de Valencia, Spain) Alessandra Feo (Università di Parma, Italy) Dario Francia (Università di Roma Tor Vergata, Italy) Francesco Fucito (Università di Roma Tor Vergata, Italy) Dmitri Fursaev (JINR, Dubna, Russia) Daniel Galehouse (University of Akron, Ohio, USA) Remo Garattini (Università di Bergamo, Italy) Florian Girelli (Perimeter Institute, Waterloo, Canada) Luca Griguolo (Università di Parma, Italy) Daniel Grumiller (Universität Leipzig, Germany) Shinichi Horata (Hayama Center of Advanced Research, Japan) Giorgio Immirzi (Università di Perugia, Italy) Roman Jackiw (MIT, Cambridge, USA) Matyas Karadi (DAMTP, University of Cambridge, UK) Mikhail Katanaev (Steklov Mathematical Institute, Moscow, Russia) Claus Kiefer (Universität Koln, Germany) John Klauder (University of Florida, Gainesville, USA) Pavel Klepac (Masaryk University, Brno, Czech Republic) Jen-Chi Lee (National Chiao-Tung University, Taiwan) Carlos Leiva (Universidad de Tarapacá, Arica, Chile) Stefano Liberati (SISSA/ISAS, Trieste, Italy) Jorma Louko (University of Nottingham, UK) Luca Lusanna (INFN, Sezione di Firenze, Italy) Roy Maartens (University of Portsmouth, UK) Fotini Markopoulou (Perimeter Institute, Waterloo, Canada) Annalisa Marzuoli (Università di Pavia, Italy) Evangelos Melas (QMW, University of London, UK) Maurizio Melis (Università di Cagliary, Italy) Vitaly Melnikov (VNIIMS, Moscow, Russia) Guillermo A. Mena Marugan (CSIC, Madrid, Spain) Pietro Menotti (Università di Pisa, Italy) Salvatore Mignemi (Università di Cagliari, Italy) Aleksandar Mikovic (Universidade Lusófona, Lisboa, Portugal) Leonardo Modesto (Université de la Mediterranée, Marseille