Distributed Autonomous Control Action Based on Sensor and Mission Fusion

DTIC Science & Technology

2005-09-01

programmable control algorithm driven by the readings of two pressure switch sensors located on either side of the valve unit. Thus, a micro-controller...and Characterization The process of leak detection and characterization must be accomplished with a set of pressure switch sensors. This sensor...economically supplementing existing widely used pressure switch type sensors which are characterized by prohibitively long inertial lag responses

Data set on the effects of conifer control and slash burning on soil carbon, total N, organic matter and extractable micro-nutrients

USDA-ARS?s Scientific Manuscript database

Conifer control in sagebrush steppe of the western United States causes various levels of site disturbance influencing vegetation recovery and resource availability. The data presented in this article are growing season availability of soil micronutrients and levels of total soil carbon, organic ma...

Control technology for integrated circuit fabrication at Micro-Circuit Engineering, Incorporated, West Palm Beach, Florida

NASA Astrophysics Data System (ADS)

Mihlan, G. I.; Mitchell, R. I.; Smith, R. K.

1984-07-01

A survey to assess control technology for integrated circuit fabrication was conducted. Engineering controls included local and general exhaust ventilation, shielding, and personal protective equipment. Devices or work stations that contained toxic materials that were potentially dangerous were controlled by local exhaust ventilation. Less hazardous areas were controlled by general exhaust ventilation. Process isolation was used in the plasma etching, low pressure chemical vapor deposition, and metallization operations. Shielding was used in ion implantation units to control X-ray emissions, in contact mask alignes to limit ultraviolet (UV) emissions, and in plasma etching units to control radiofrequency and UV emissions. Most operations were automated. Use of personal protective equipment varied by job function.

Factors Affecting the Discharge of Micro-Plastic Fibers from Household Laundry

NASA Astrophysics Data System (ADS)

Lange, N.

2017-12-01

Every day millions of loads of laundry are done in in the United States alone. Many, if not most, include synthetic fibers. During washing, micro-plastic fibers are released from the fabric, and discharged into the wastewater. These fibers have been detected in fresh water throughout the world and all of the oceans. These micro-plastic fibers are an emerging environmental contaminant that can adversely affect wildlife and are highly bio-accumulated in aquatic food-chains. Additionally, like other plastics, micro-fibers are not readily biodegraded and persist in the environment for a long time. In this research, I explored the effect of the way we wash clothes on the amount of micro-plastic fibers that are shed by common clothing materials containing man-made fibers. I collected discharge samples from wash and rinse cycles of a washing machine. I collected samples from a control wash using no detergent and then repeated five times. Next, I repeated the experiment five times using four different types of detergent. Large amounts of micro-plastic fibers were released during all wash cycles. However, the numbers decreased during the later rinse cycles. The use of laundry detergent increased the number of micro-plastic fibers released into the wash-water. Deep cleaning detergents produced over ten times more fibers than the no-detergent control. The gentlest detergent only released two times more fibers than the control. Therefore, it would be possible to affect the number of fibers released into the wastewater simply by selection of detergent. The ultimate goal of my research is to develop an optimized detergent that minimizes the number of micro-plastic fibers generated by washing and still effectively clean clothes.

Modelling and control synthesis of a micro-combined heat and power interface for a concentrating solar power system in off-grid rural power applications

NASA Astrophysics Data System (ADS)

Prinsloo, Gerro; Dobson, Robert; Brent, Alan; Mammoli, Andrea

2016-05-01

Concentrating solar power co-generation systems have been identified as potential stand-alone solar energy supply solutions in remote rural energy applications. This study describes the modelling and synthesis of a combined heat and power Stirling CSP system in order to evaluate its potential performance in small off-grid rural village applications in Africa. This Stirling micro-Combined Heat and Power (micro-CHP) system has a 1 kW electric capacity, with 3 kW of thermal generation capacity which is produced as waste heat recovered from the solar power generation process. As part of the development of an intelligent microgrid control and distribution solution, the Trinum micro-CHP system and other co-generation systems are systematically being modelled on the TRNSYS simulation platform. This paper describes the modelling and simulation of the Trinum micro-CHP configuration on TRNSYS as part of the process to develop the control automation solution for the smart rural microgrid in which the Trinum will serve as a solar powerpack. The results present simulated performance outputs for the Trinum micro-CHP system for a number of remote rural locations in Africa computed from real-time TRNSYS solar irradiation and weather data (yearly, monthly, daily) for the relevant locations. The focus of this paper is on the parametric modelling of the Trinum Stirling micro-CHP system, with specific reference to this system as a TRNSYS functional block in the microgrid simulation. The model is used to forecast the solar energy harvesting potential of the Trinum micro-CHP unit at a number of remote rural sites in Africa.

Development of an Inertial Measurement Unit for Unmanned Aerial Vehicles

DTIC Science & Technology

2011-02-01

Tri-axis accelerometers Dual Angular rate gyros Micro-Controller board Battery Rubber isolation stands wireless comunication module (XBee...University. Special thanks to Mr. Jesse McAvoy & Mr. Laith Sahawneh for all the support and valuable scientific contributions. gxk gyk gzk gxb

Comparison of competitive exclusion with classical cleaning and disinfection on bacterial load in pig nursery units.

PubMed

Luyckx, K; Millet, S; Van Weyenberg, S; Herman, L; Heyndrickx, M; Dewulf, J; De Reu, K

2016-09-06

Colonisation of the environment of nursery units by pathogenic micro-organisms is an important factor in the persistence and spread of endemic diseases in pigs and zoonotic pathogens. These pathogens are generally controlled by the use of antibiotics and disinfectants. Since an increasing resistance against these measures has been reported in recent years, methods such as competitive exclusion (CE) are promoted as promising alternatives. This study showed that the infection pressure in CE units after microbial cleaning was not reduced to the same degree as in control units. Despite sufficient administration of probiotic-type spores, the analysed bacteria did not decrease in number after 3 production rounds in CE units, indicating no competitive exclusion. In addition, no differences in feed conversion were found between piglets raised in CE and control units in our study. Also, no differences in faecal consistency (indicator for enteric diseases) was noticed. These results indicate that the CE protocol is not a valuable alternative for classical C&D.

Effect of a multi-layer infection control barrier on the micro-hardness of a composite resin

PubMed Central

HWANG, In-Nam; HONG, Sung-Ok; LEE, Bin-Na; HWANG, Yun-Chan; OH, Won-Mann; CHANG, Hoon-Sang

2012-01-01

Objective The aim of this study was to evaluate the effect of multiple layers of an infection control barrier on the micro-hardness of a composite resin. Material and Methods One, two, four, and eight layers of an infection control barrier were used to cover the light guides of a high-power light emitting diode (LED) light curing unit (LCU) and a low-power halogen LCU. The composite specimens were photopolymerized with the LCUs and the barriers, and the micro-hardness of the upper and lower surfaces was measured (n=10). The hardness ratio was calculated by dividing the bottom surface hardness of the experimental groups by the irradiated surface hardness of the control groups. The data was analyzed by two-way ANOVA and Tukey's HSD test. Results The micro-hardness of the composite specimens photopolymerized with the LED LCU decreased significantly in the four- and eight-layer groups of the upper surface and in the two-, four-, and eight-layer groups of the lower surface. The hardness ratio of the composite specimens was <80% in the eight-layer group. The micro-hardness of the composite specimens photopolymerized with the halogen LCU decreased significantly in the eight-layer group of the upper surface and in the two-, four-, and eight-layer groups of the lower surface. However, the hardness ratios of all the composite specimens photopolymerized with barriers were <80%. Conclusions The two-layer infection control barrier could be used on high-power LCUs without decreasing the surface hardness of the composite resin. However, when using an infection control barrier on the low-power LCUs, attention should be paid so as not to sacrifice the polymerization efficiency. PMID:23138746

Decentralized and Modular Electrical Architecture

NASA Astrophysics Data System (ADS)

Elisabelar, Christian; Lebaratoux, Laurence

2014-08-01

This paper presents the studies made on the definition and design of a decentralized and modular electrical architecture that can be used for power distribution, active thermal control (ATC), standard inputs-outputs electrical interfaces.Traditionally implemented inside central unit like OBC or RTU, these interfaces can be dispatched in the satellite by using MicroRTU.CNES propose a similar approach of MicroRTU. The system is based on a bus called BRIO (Bus Réparti des IO), which is composed, by a power bus and a RS485 digital bus. BRIO architecture is made with several miniature terminals called BTCU (BRIO Terminal Control Unit) distributed in the spacecraft.The challenge was to design and develop the BTCU with very little volume, low consumption and low cost. The standard BTCU models are developed and qualified with a configuration dedicated to ATC, while the first flight model will fly on MICROSCOPE for PYRO actuations and analogue acquisitions. The design of the BTCU is made in order to be easily adaptable for all type of electric interface needs.Extension of this concept is envisaged for power conditioning and distribution unit, and a Modular PCDU based on BRIO concept is proposed.

Micro-cogeneration units based on Stirling engine for heating and their real operation

NASA Astrophysics Data System (ADS)

Čierny, Jaroslav; Patsch, Marek

2014-08-01

This article was deal with micro-cogeneration units based on Stirling engine. We watched problematic of real working Stirling engine. The article also contain hookup of unit constructed at University of Zilina.

Microfabrication and Test of a Three-Dimensional Polymer Hydro-Focusing Unit for Flow Cytometry Applications

NASA Technical Reports Server (NTRS)

Yang, Ren; Feedback, Daniel L.; Wang, Wanjun

2004-01-01

This paper details a novel three-dimensional (3D) hydro-focusing micro cell sorter for micro flow cytometry applications. The unit was micro-fabricated by means of SU-8 3D lithography. The 3D microstructure for coaxial sheathing was designed, micro-fabricated, and tested. Three-dimensional hydrofocusing capability was demonstrated with an experiment to sort labeled tanned sheep erythrocytes (red blood cells). This polymer hydro-focusing microstructure is easily micro-fabricated and integrated with other polymer microfluidic structures.

77 FR 24766 - Call for Proposals for a Micro Support Program on International Conflict Resolution and...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-25

... UNITED STATES INSTITUTE OF PEACE Call for Proposals for a Micro Support Program on International Conflict Resolution and Peacebuilding For Immediate Release AGENCY: United States Institute of Peace. ACTION: Notice. SUMMARY: Micro Support Program on International Conflict Resolution and Peacebuilding...

A Lab-on-Chip Design for Miniature Autonomous Bio-Chemoprospecting Planetary Rovers

NASA Astrophysics Data System (ADS)

Santoli, S.

The performance of the so-called ` Lab-on-Chip ' devices, featuring micrometre size components and employed at present for carrying out in a very fast and economic way the extremely high number of sequence determinations required in genomic analyses, can be largely improved as to further size reduction, decrease of power consumption and reaction efficiency through development of nanofluidics and of nano-to-micro inte- grated systems. As is shown, such new technologies would lead to robotic, fully autonomous, microwatt consumption and complete ` laboratory on a chip ' units for accurate, fast and cost-effective astrobiological and planetary exploration missions. The theory and the manufacturing technologies for the ` active chip ' of a miniature bio/chemoprospecting planetary rover working on micro- and nanofluidics are investigated. The chip would include micro- and nanoreactors, integrated MEMS (MicroElectroMechanical System) components, nanoelectronics and an intracavity nanolaser for highly accurate and fast chemical analysis as an application of such recently introduced solid state devices. Nano-reactors would be able to strongly speed up reaction kinetics as a result of increased frequency of reactive collisions. The reaction dynamics may also be altered with respect to standard macroscopic reactors. A built-in miniature telemetering unit would connect a network of other similar rovers and a central, ground-based or orbiting control unit for data collection and transmission to an Earth-based unit through a powerful antenna. The development of the ` Lab-on-Chip ' concept for space applications would affect the economy of space exploration missions, as the rover's ` Lab-on-Chip ' development would link space missions with the ever growing terrestrial market and business concerning such devices, largely employed in modern genomics and bioinformatics, so that it would allow the recoupment of space mission costs.

The survival of micro-organisms in space. Further rocket and balloon-borne exposure experiments.

PubMed

Hotchin, J; Lorenz, P; Markusen, A; Hemenway, C

1967-01-01

This report describes the results of survival studies of terrestrial micro-organisms exposed directly to the space environment on two balloons and in two rocket flights. The work is part of a program to develop techniques for the collection of micro-organisms in the size range of micrometeorite particles in space or non-terrestrial atmospheres, and their return to earth in a viable state for further study. Previous survival studies were reported (J. Hotchin, P. Lorenz and C. Hemenway, Nature 206 (1965) 442) in which a few relatively large area samples of micro-organisms were exposed on millipore filter cemented to aluminum plates. In the present series of experiments, newly developed techniques have resulted in a 25-fold miniaturization resulting in a corresponding increase in the number of experiments performed. This has enabled a statistical evaluation of the results to be made. A total of 756 separate exposure units (each approximately 5 x 5 mm in size) were flown in four experiments, and organisms used were coliphage T1, penicillium roqueforti (THOM) mold spores, poliovirus type I (Pfizer attenuated Sabin vaccine strain), and bacillus subtilis spores. The organisms were deposited either by spraying directly upon the vinyl-coated metal units, or by droplet seeding into shallow depressions in the millipore filter membrane-coated units. Groups of units were prepared comprising fully exposed, inverted (screened by 2 mm of Al), and filter-protected organisms. All of these were included in the flight set, the back up set, and a laboratory control set. The altitude of the exposures varied from 35 km in the balloon experiments to 150 km in the rocket experiments. Times of exposures at altitude were approximately 6 hours for the balloon flights and about 3 minutes for the rocket experiments.

Thrust Generation with Low-Power Continuous-Wave Laser and Aluminum Foil Interaction

DOE Office of Scientific and Technical Information (OSTI.GOV)

Horisawa, Hideyuki; Sumida, Sota; Funaki, Ikkoh

2010-05-06

The micro-newton thrust generation was observed through low-power continuous-wave laser and aluminum foil interaction without any remarkable ablation of the target surface. To evaluate the thrust characteristics, a torsion-balance thrust stand capable for the measurement of the thrust level down to micro-Newton ranges was developed. In the case of an aluminum foil target with 12.5 micrometer thickness, the maximum thrust level was 15 micro-newtons when the laser power was 20 W, or about 0.75 N/MW. It was also found that the laser intensity, or laser power per unit area, irradiated on the target was significantly important on the control ofmore » the thrust even under the low-intensity level.« less

An extensible, low-cost drifter control unit

NASA Astrophysics Data System (ADS)

Giudici, Andrea; Torsvik, Tomas; Soomere, Tarmo

2017-04-01

We introduce an extensible, low-cost drifter control unit, which is developed for use with surface drifters that are deployed in inland water bodies or near-coast regions. The control unit is custom-built on top of a small footprint micro controller. It makes use of a GPS receiver for position tracking, a GSM radio for data transmission, and two sensor buses to handle analog and digital data measured by an array of external sensors. A cloud-based data collection platform receives and stores the data transmitted over GPRS from the drifter. The control unit was found to perform satisfactorily in operational testing, providing data at sub-Hz frequency for position and temperature during extended time. Test deployments revealed some issues related to power consumption spikes. Even though the unit is fully functional in the present form and shows, on average, low energy consumption , battery packs with relatively large maximum output power are required to ensure its operation within prolonged periods of time. We expect that a further development of the power supply unit together with a careful de-synchronization scheme of sensors' operation would smooth those peaks without any loss of the quality of recorded information.

Effect of simulated microgravity on oxidation-sensitive gene expression in PC12 cells

NASA Astrophysics Data System (ADS)

Kwon, Ohwon; Sartor, Maureen; Tomlinson, Craig R.; Millard, Ronald W.; Olah, Mark E.; Sankovic, John M.; Banerjee, Rupak K.

2006-01-01

Oxygen utilization by and oxygen dependence of cellular processes may be different in biological systems that are exposed to microgravity (micro-g). A baseline in which cellular changes in oxygen sensitive molecular processes occur during micro-g conditions would be important to pursue this question. The objective of this research is to analyze oxidation-sensitive gene expression in a model cell line [rat pheochromocytoma (PC12)] under simulated micro-g conditions. The PC12 cell line is well characterized in its response to oxygen, and is widely recognized as a sensitive model for studying the responses of oxygen-sensitive molecular and cellular processes. This study uses the rotating wall vessel bioreactor (RWV) designed at NASA to simulate micro-g. Gene expression in PC12 cells in response to micro-g was analyzed by DNA microarray technology. The microarray analysis of PC12 cells cultured for 4 days under simulated micro-g under standardized oxygen environment conditions revealed more than 100 genes whose expression levels were changed at least twofold (up-regulation of 65 genes and down-regulation of 39 genes) compared with those from cells in the unit gravity (unit-g) control. This study observed that genes involved in the oxidoreductase activity category were most significantly differentially expressed under micro-g conditions. Also, known oxidation-sensitive transcription factors such as hypoxia-inducible factor-2α, c-myc, and the peroxisome proliferator-activated receptor-γ were changed significantly. Our initial results from the gene expression microarray studies may provide a context in which to evaluate the effect of varying oxygen environments on the background of differential gene regulation of biological processes under variable gravity conditions.

Effect of simulated microgravity on oxidation-sensitive gene expression in PC12 cells

PubMed Central

Kwon, Ohwon; Sartor, Maureen; Tomlinson, Craig R.; Millard, Ronald W.; Olah, Mark E.; Sankovic, John M.; Banerjee, Rupak K.

2008-01-01

Oxygen utilization by and oxygen dependence of cellular processes may be different in biological systems that are exposed to microgravity (micro-g). A baseline in which cellular changes in oxygen sensitive molecular processes occur during micro-g conditions would be important to pursue this question. The objective of this research is to analyze oxidation-sensitive gene expression in a model cell line [rat pheochromocytoma (PC12)] under simulated micro-g conditions. The PC12 cell line is well characterized in its response to oxygen, and is widely recognized as a sensitive model for studying the responses of oxygen-sensitive molecular and cellular processes. This study uses the rotating wall vessel bioreactor (RWV) designed at NASA to simulate micro-g. Gene expression in PC12 cells in response to micro-g was analyzed by DNA microarray technology. The microarray analysis of PC12 cells cultured for 4 days under simulated micro-g under standardized oxygen environment conditions revealed more than 100 genes whose expression levels were changed at least twofold (up-regulation of 65 genes and down-regulation of 39 genes) compared with those from cells in the unit gravity (unit-g) control. This study observed that genes involved in the oxidoreductase activity category were most significantly differentially expressed under micro-g conditions. Also, known oxidation-sensitive transcription factors such as hypoxia-inducible factor-2α, c-myc, and the peroxisome proliferator-activated receptor-γ were changed significantly. Our initial results from the gene expression microarray studies may provide a context in which to evaluate the effect of varying oxygen environments on the background of differential gene regulation of biological processes under variable gravity conditions. PMID:19081771

[Integrated Development of Full-automatic Fluorescence Analyzer].

PubMed

Zhang, Mei; Lin, Zhibo; Yuan, Peng; Yao, Zhifeng; Hu, Yueming

2015-10-01

In view of the fact that medical inspection equipment sold in the domestic market is mainly imported from abroad and very expensive, we developed a full-automatic fluorescence analyzer in our center, presented in this paper. The present paper introduces the hardware architecture design of FPGA/DSP motion controlling card+PC+ STM32 embedded micro processing unit, software system based on C# multi thread, design and implementation of double-unit communication in detail. By simplifying the hardware structure, selecting hardware legitimately and adopting control system software to object-oriented technology, we have improved the precision and velocity of the control system significantly. Finally, the performance test showed that the control system could meet the needs of automated fluorescence analyzer on the functionality, performance and cost.

Injection-depth-locking axial motion guided handheld micro-injector using CP-SSOCT.

PubMed

Cheon, Gyeong Woo; Huang, Yong; Kwag, Hye Rin; Kim, Ki-Young; Taylor, Russell H; Gehlbach, Peter L; Kang, Jin U

2014-01-01

This paper presents a handheld micro-injector system using common-path swept source optical coherence tomography (CP-SSOCT) as a distal sensor with highly accurate injection-depth-locking. To achieve real-time, highly precise, and intuitive freehand control, the system used graphics processing unit (GPU) to process the oversampled OCT signal with high throughput and a smart customized motion monitoring control algorithm. A performance evaluation was conducted with 60-insertions and fluorescein dye injection tests to show how accurately the system can guide the needle and lock to the target depth. The evaluation tests show our system can guide the injection needle into the desired depth with 4.12 um average deviation error while injecting 50 nl of fluorescein dye.

Identification of infusion strategy for achieving repeatable nanoparticle distribution and quantification of thermal dosage using micro-CT Hounsfield unit in magnetic nanoparticle hyperthermia.

PubMed

LeBrun, Alexander; Joglekar, Tejashree; Bieberich, Charles; Ma, Ronghui; Zhu, Liang

2016-01-01

The objective of this study was to identify an injection strategy leading to repeatable nanoparticle deposition patterns in tumours and to quantify volumetric heat generation rate distribution based on micro-CT Hounsfield unit (HU) in magnetic nanoparticle hyperthermia. In vivo animal experiments were performed on graft prostatic cancer (PC3) tumours in immunodeficient mice to investigate whether lowering ferrofluid infusion rate improves control of the distribution of magnetic nanoparticles in tumour tissue. Nanoparticle distribution volume obtained from micro-CT scan was used to evaluate spreading of the nanoparticles from the injection site in tumours. Heating experiments were performed to quantify relationships among micro-CT HU values, local nanoparticle concentrations in the tumours, and the ferrofluid-induced volumetric heat generation rate (q(MNH)) when nanoparticles were subject to an alternating magnetic field. An infusion rate of 3 µL/min was identified to result in the most repeatable nanoparticle distribution in PC3 tumours. Linear relationships have been obtained to first convert micro-CT greyscale values to HU values, then to local nanoparticle concentrations, and finally to nanoparticle-induced q(MNH) values. The total energy deposition rate in tumours was calculated and the observed similarity in total energy deposition rates in all three infusion rate groups suggests improvement in minimising nanoparticle leakage from the tumours. The results of this study demonstrate that micro-CT generated q(MNH) distribution and tumour physical models improve predicting capability of heat transfer simulation for designing reliable treatment protocols using magnetic nanoparticle hyperthermia.

Oximeter reliability in a subzero environment.

PubMed

Macnab, A J; Smith, M; Phillips, N; Smart, P

1996-11-01

Pulse oximeters optimize care in the pre-hospital setting. As British Columbia ambulance teams often provide care in subzero temperatures, we conducted a study to determine the reliability of 3 commercially-available portable oximeters in a subzero environment. We hypothesized that there is no significant difference between SaO2 readings obtained using a pulse oximeter at room temperature and a pulse oximeter operating at sub-zero temperatures. Subjects were stable normothermic children in intensive care on Hewlett Packard monitors (control unit) at room temperature. The test units were packed in dry ice in an insulated bin (temperature - 15 degrees C to -30 degrees C) and their sensors placed on the subjects, contralateral to the control sensors. Data were collected simultaneously from test and control units immediately following validation of control unit values by co-oximetry (blood gas). No data were unacceptable. Two units (Propaq 106EC and Nonin 8500N) functioned well to < -15 degrees C, providing data comparable to those obtained from the control unit (p < 0.001). The Siemens Micro O2 did not function at the temperatures tested. Monitor users who require equipment to function in subzero environments (military, Coast Guard, Mountain Rescue) should ensure that function is reliable, and could test units using this method.

Nondestructive Evaluation of Metallized Tape Bonds Formed by Tape Automated Bonding (TAB)

DTIC Science & Technology

1989-04-01

powered by micro-positioning linear actuators. 3) Interchangeable sample-holding fixtures mounted upon top of slide assembly. 4) Coverslip gantry mounted...Controller Unit 1) Motor power supplies 2) Motor output servo driver amplifiers 3) "Macro-language" command Interpreter 4) Two-way cormunications with...adjustments are manual knobs giving approximately one degree of tilt adjustment per turn. The servo controller has self-contained power supplies for

An efficient micro control unit with a reconfigurable filter design for wireless body sensor networks (WBSNs).

PubMed

Chen, Chiung-An; Chen, Shih-Lun; Huang, Hong-Yi; Luo, Ching-Hsing

2012-11-22

In this paper, a low-cost, low-power and high performance micro control unit (MCU) core is proposed for wireless body sensor networks (WBSNs). It consists of an asynchronous interface, a register bank, a reconfigurable filter, a slop-feature forecast, a lossless data encoder, an error correct coding (ECC) encoder, a UART interface, a power management (PWM), and a multi-sensor controller. To improve the system performance and expansion abilities, the asynchronous interface is added for handling signal exchanges between different clock domains. To eliminate the noise of various bio-signals, the reconfigurable filter is created to provide the functions of average, binomial and sharpen filters. The slop-feature forecast and the lossless data encoder is proposed to reduce the data of various biomedical signals for transmission. Furthermore, the ECC encoder is added to improve the reliability for the wireless transmission and the UART interface is employed the proposed design to be compatible with wireless devices. For long-term healthcare monitoring application, a power management technique is developed for reducing the power consumption of the WBSN system. In addition, the proposed design can be operated with four different bio-sensors simultaneously. The proposed design was successfully tested with a FPGA verification board. The VLSI architecture of this work contains 7.67-K gate counts and consumes the power of 5.8 mW or 1.9 mW at 100 MHz or 133 MHz processing rate using a TSMC 0.18 μm or 0.13 μm CMOS process. Compared with previous techniques, this design achieves higher performance, more functions, more flexibility and higher compatibility than other micro controller designs.

The Role of Design-of-Experiments in Managing Flow in Compact Air Vehicle Inlets

NASA Technical Reports Server (NTRS)

Anderson, Bernhard H.; Miller, Daniel N.; Gridley, Marvin C.; Agrell, Johan

2003-01-01

It is the purpose of this study to demonstrate the viability and economy of Design-of-Experiments methodologies to arrive at microscale secondary flow control array designs that maintain optimal inlet performance over a wide range of the mission variables and to explore how these statistical methods provide a better understanding of the management of flow in compact air vehicle inlets. These statistical design concepts were used to investigate the robustness properties of low unit strength micro-effector arrays. Low unit strength micro-effectors are micro-vanes set at very low angles-of-incidence with very long chord lengths. They were designed to influence the near wall inlet flow over an extended streamwise distance, and their advantage lies in low total pressure loss and high effectiveness in managing engine face distortion. The term robustness is used in this paper in the same sense as it is used in the industrial problem solving community. It refers to minimizing the effects of the hard-to-control factors that influence the development of a product or process. In Robustness Engineering, the effects of the hard-to-control factors are often called noise , and the hard-to-control factors themselves are referred to as the environmental variables or sometimes as the Taguchi noise variables. Hence Robust Optimization refers to minimizing the effects of the environmental or noise variables on the development (design) of a product or process. In the management of flow in compact inlets, the environmental or noise variables can be identified with the mission variables. Therefore this paper formulates a statistical design methodology that minimizes the impact of variations in the mission variables on inlet performance and demonstrates that these statistical design concepts can lead to simpler inlet flow management systems.

Electrostatic Accelerometer for the Gravity Recovery and Climate Experiment Follow-On Mission (GRACE FO)

NASA Astrophysics Data System (ADS)

Perrot, Eddy; Boulanger, Damien; Christophe, Bruno; Foulon, Bernard; Liorzou, Françoise; Lebat, Vincent

2014-05-01

The GRACE FO mission, led by the JPL (Jet Propulsion Laboratory), is an Earth-orbiting gravity mission, continuation of the GRACE mission, that will produce an accurate model of the Earth's gravity field variation providing global climatic data during five year at least. The mission involves two satellites in a loosely controlled tandem formation, with a micro-wave link, and optionally a laser link, measuring the inter-satellites distance variation. Non-uniformities in the distribution of the Earth's mass cause the distance between the two satellites to vary. This variation is measured to recover gravity, after subtracting the non-gravitational contributors, as the residual drag. ONERA (the French Aerospace Lab) is developing, manufacturing and testing electrostatic accelerometers measuring this residual drag applied on the satellites. The accelerometer is composed of two main parts: the Sensor Unit (including the Sensor Unit Mechanics - SUM - and the Front-End Electronic Unit - FEEU) and the Interface Control Unit. In the Accelerometer Core, located in the Sensor Unit Mechanics, the proof mass is levitated and maintained in a center of an electrode cage by electrostatic forces. Thus, any drag acceleration applied on the satellite involves a variation on the servo-controlled electrostatic suspension of the mass. The voltage on the electrodes providing this electrostatic force is the measurement output of the accelerometer. The Preliminary Design Review was achieved successfully on November 2013. The FEEU Engineering Model is under test. Preliminary results on electronic unit will be compared with the expected performance. The integration of the SUM Engineering Model and the first ground levitation of the proof-mass will be presented. The impact of the accelerometer defaults (geometry, electronic and parasitic forces) leads to bias, misalignment and scale factor error, non-linearity and noise. Some of these accelerometer defaults are characterized by tests with micro-gravity pendulum bench and with drops in ZARM catapult. The post-processing needed to achieve the performance, in particular with regards to the temperature stability, will be explained.

A modular assembly method of a feed and thruster system for Cubesats

NASA Astrophysics Data System (ADS)

Louwerse, Marcus; Jansen, Henri; Elwenspoek, Miko

2010-11-01

A modular assembly method for devices based on micro system technology is presented. The assembly method forms the foundation for a miniaturized feed and thruster system as part of a micro propulsion unit working as a simple blow-down system of a rocket engine. The micro rocket is designed to be used for constellation maintenance of Cubesats, which measure 10 × 10 × 10 cm and have a mass less than 1 kg. The feed and thruster system contains an active valve, control electronics, a particle filter and an axisymmetric converging-diverging nozzle, all fabricated as separate modules. A novel method is used to integrate these modules by placing them on or in a glass tube package. The assembly method is shown to be a valid method but the valve module needs to be improved considerably.

Effects of ceftazidime, a betalactam antibiotic, on murine haemopoiesis in vitro.

PubMed

Hauser, S P; Udupa, K B; Lipschitz, D A

1994-04-01

Agranulocytosis has been reported in 5-15% of patients treated with high-dose betalactam antibiotics (BLA). We investigated the toxic effect of ceftazidime (CEF) as a representative of these antibiotics on colony-forming unit-granulocyte/macrophage (CFU-GM), on burst-forming unit-erythroid (BFU-E) colony growth and on myelopoiesis in murine long-term bone marrow culture (mLTBMC). The CEF concentration resulting in a 50% inhibition of growth was 146 micrograms/ml (267 microM) for CFU-GM, 132 micrograms/ml (241 microM) for BFU-E and 180 micrograms/ml (329 microM) for myeloid cell production in the supernatant of mLTBMC. Following addition of CEF to mLTBMC, CFU-GM remained low for 1 week and total myeloid cell production remained low for 2 weeks after removal of CEF from culture. Thereafter the values returned to control levels. The myeloid differential counts in the supernatant and adherent layers demonstrated a 'maturation arrest', which could be overcome by simultaneously adding all-trans retinoic acid to culture. These results demonstrate that CEF has reversible inhibitory effects on myelopoiesis and highlight the utility of in vitro haemopoietic assays as models to examine drug-induced haemopoietic dyscrasias.

Ferrofluid based micro-electrical energy harvesting

NASA Astrophysics Data System (ADS)

Purohit, Viswas; Mazumder, Baishakhi; Jena, Grishma; Mishra, Madhusha; Materials Department, University of California, Santa Barbara, CA93106 Collaboration

2013-03-01

Innovations in energy harvesting have seen a quantum leap in the last decade. With the introduction of low energy devices in the market, micro energy harvesting units are being explored with much vigor. One of the recent areas of micro energy scavenging is the exploitation of existing vibrational energy and the use of various mechanical motions for the same, useful for low power consumption devices. Ferrofluids are liquids containing magnetic materials having nano-scale permanent magnetic dipoles. The present work explores the possibility of the use of this property for generation of electricity. Since the power generation is through a liquid material, it can take any shape as well as response to small acceleration levels. In this work, an electromagnet-based micropower generator is proposed to utilize the sloshing of the ferrofluid within a controlled chamber which moves to different low frequencies. As compared to permanent magnet units researched previously, ferrofluids can be placed in the smallest of containers of different shapes, thereby giving an output in response to the slightest change in motion. Mechanical motion from 1- 20 Hz was able to give an output voltage in mV's. In this paper, the efficiency and feasibility of such a system is demonstrated.

Biocompatibility Analysis of an Electrically-Activated Silver-Based Antibacterial Surface System for Medical Device Applications

DTIC Science & Technology

2012-12-16

sterilizing without causing toxicity in vivo. 1 Introduction As reported to the Centers for Disease Control and Pre- vention (CDC) between 2006 and...Owings MF. National Hospital Discharge Survey. Advance Data from Vital and Health Statistics. United States: Centers for Disease Control and Prevention...10.1007/s10856-012-4730-3. 19. Shirwaiker RA, Wysk RA, Kariyawasam S, Carrion H, Voigt RC. Micro-scale fabrication and characterization of a silver–polymer

Method and apparatus for actively controlling a micro-scale flexural plate wave device

DOEpatents

Dohner, Jeffrey L.

2001-01-01

An actively controlled flexural plate wave device provides a micro-scale pump. A method of actively controlling a flexural plate wave device produces traveling waves in the device by coordinating the interaction of a magnetic field with actively controlled currents. An actively-controlled flexural plate wave device can be placed in a fluid channel and adapted for use as a micro-scale fluid pump to cool or drive micro-scale systems, for example, micro-chips, micro-electrical-mechanical devices, micro-fluid circuits, or micro-scale chemical analysis devices.

Design of an intelligent flight instrumentation unit using embedded RTOS

NASA Astrophysics Data System (ADS)

Estrada-Marmolejo, R.; García-Torales, G.; Torres-Ortega, H. H.; Flores, J. L.

2011-09-01

Micro Unmanned Aerial Vehicles (MUAV) must calculate its spatial position to control the flight dynamics, which is done by Inertial Measurement Units (IMUs). MEMS Inertial sensors have made possible to reduce the size and power consumption of such units. Commonly the flight instrumentation operates independently of the main processor. This work presents an instrumentation block design, which reduces size and power consumption of the complete system of a MUAV. This is done by coupling the inertial sensors to the main processor without considering any intermediate level of processing aside. Using Real Time Operating Systems (RTOS) reduces the number of intermediate components, increasing MUAV reliability. One advantage is the possibility to control several different sensors with a single communication bus. This feature of the MEMS sensors makes a smaller and less complex MUAV design possible.

Micro guidance and control technology overview

NASA Technical Reports Server (NTRS)

Kissel, Glen J.; Hadaegh, Fred Y.

1993-01-01

This paper gives an overview of micro-guidance and control technologies and in the process previews of the technology/user and systems issues presented in the guidance and control session at the workshop. We first present a discussion of the advantages of using micro-guidance and control components and then detail six micro-guidance and control thrusts that could have a revolutionary impact on space missions and systems. Specific technologies emerging in the micro-guidance and control field will be examined. These technologies fall into two broad categories: micro-attitude determination (inertial and celestial) and micro-actuation, control and sensing. Finally, the scope of the workshop's guidance and control panel are presented.

Micro-Masonry: Construction of 3D Structures by Mesoscale Self-Assembly

PubMed Central

Fernandez, Javier G.; Khademhosseini, Ali

2010-01-01

A general method for construction of three dimensional structures by directed assembly of microscale polymeric sub-units is presented. Shape-controlled microgels are directed to assemble into different shapes by limiting their movement onto a molded substrate. The capillary forces, resulting from the presence of a liquid polymer, assemble the microgels in close contact with the rest of the units and with the free surface, the latter imposing the final geometry of the resulting construct. The result is a freestanding structure composed of one or multiple layers of sub-units assembled in a tightly packed conformation. The applicability of the technique for the construction of scaffolds with cell-laden sub-units is demonstrated. In addition, scaffolds formed by the sequential aggregation of sub-units are produced. PMID:20440697

Improvement of the Power Control Unit for Ion Thruster to Cope with Milli-Newton Range RIT

NASA Astrophysics Data System (ADS)

Ceruti, Luca; Polli, Aldo; Galantini, Paolo

2014-08-01

The recent development and testing activities of a miniaturized Radio-Frequency Ion Thruster, with relevant ancillary elements, in the range of 10 to 100 micro-Newtons, joined with past flight heritage in the milli-Newton range (RIT-10 for Artemis), shows an appealing capability of such an electrical propulsion technology to support thrust in a wide range of space applications from very fine attitude control up to deorbiting of small-medium satellites. As expectable, this implies that the mentioned ancillary elements (mainly Radio-Frequency Generator and Power Control Unit) require adaptation to the different requirements imposed to different missions and thrust ranges. Regarding the Power Control Unit different power levels, both the controllability requirements and the spacecraft interfaces impose non negligible adaptation leading to significant increase of development activities and associated cost (nonrecurring) increase. From that and with the main purpose to minimize such impacts and provide reliable equipments, Selex ES since a few years is devoting maximum attention in the incremental innovation of the existing design in order to maximize their reuse.

Status of Electrostatic Accelerometer Development for Gravity Recovery and Climate Experiment Follow-On Mission (GRACE FO)

NASA Astrophysics Data System (ADS)

Perrot, Eddy; Boulanger, Damien; Christophe, Bruno; Foulon, Bernard; Liorzou, Françoise; Lebat, Vincent; Huynh, Phuong-Anh

2015-04-01

The GRACE FO mission, led by the JPL (Jet Propulsion Laboratory), is an Earth-orbiting gravity mission, continuation of the GRACE mission, which will produce an accurate model of the Earth's gravity field variation providing global climatic data during five years at least. The mission involves two satellites in a loosely controlled tandem formation, with a micro-wave link measuring the inter-satellites distance variation. Earth's mass distribution non-uniformities cause variations of the inter-satellite distance. This variation is measured to recover gravity, after subtracting the non-gravitational contributors, as the residual drag. ONERA (the French Aerospace Lab) is developing, manufacturing and testing electrostatic accelerometers measuring this residual drag applied on the satellites. The accelerometer is composed of two main parts: the Sensor Unit (including the Sensor Unit Mechanics - SUM - and the Front-End Electronic Unit - FEEU) and the Interface Control Unit - ICU. In the Accelerometer Core, located in the Sensor Unit Mechanics, the proof mass is levitated and maintained at the center of an electrode cage by electrostatic forces. Thus, any drag acceleration applied on the satellite involves a variation on the servo-controlled electrostatic suspension of the mass. The voltage on the electrodes providing this electrostatic force is the measurement output of the accelerometer. The impact of the accelerometer defaults (geometry, electronic and parasitic forces) leads to bias, misalignment and scale factor error, non-linearity and noise. Some of these accelerometer defaults are characterized by tests with micro-gravity pendulum bench on ground and with drops in ZARM catapult. The Critical Design Review was achieved successfully on September 2014. The Engineering Model (EM) was integrated and tested successfully, with ground levitation, drops, Electromagnetic Compatibility and thermal vacuum. The integration of the first Flight Model has begun on December 2014 and will be achieved on January 2015. The results of the Engineering Model tests and the status of the Flight Models will be presented.

Tests Results of the Electrostatic Accelerometer Flight Models for Gravity Recovery and Climate Experiment Follow-On Mission (GRACE FO)

NASA Astrophysics Data System (ADS)

Perrot, E.; Boulanger, D.; Christophe, B.; Foulon, B.; Lebat, V.; Huynh, P. A.; Liorzou, F.

2015-12-01

The GRACE FO mission, led by the JPL (Jet Propulsion Laboratory), is an Earth-orbiting gravity mission, continuation of the GRACE mission, which will produce an accurate model of the Earth's gravity field variation providing global climatic data during five years at least. The mission involves two satellites in a loosely controlled tandem formation, with a micro-wave link measuring the inter-satellites distance variation. Earth's mass distribution non-uniformities cause variations of the inter-satellite distance. This variation is measured to recover gravity, after subtracting the non-gravitational contributors, as the residual drag. ONERA (the French Aerospace Lab) is developing, manufacturing and testing electrostatic accelerometers measuring this residual drag applied on the satellites. The accelerometer is composed of two main parts: the Sensor Unit (including the Sensor Unit Mechanics - SUM - and the Front-End Electronic Unit - FEEU) and the Interface Control Unit - ICU. In the Accelerometer Core, located in the Sensor Unit Mechanics, the proof mass is levitated and maintained at the center of an electrode cage by electrostatic forces. Thus, any drag acceleration applied on the satellite involves a variation on the servo-controlled electrostatic suspension of the mass. The voltage on the electrodes providing this electrostatic force is the output measurement of the accelerometer. The impact of the accelerometer defaults (geometry, electronic and parasitic forces) leads to bias, misalignment and scale factor error, non-linearity and noise. Some of these accelerometer defaults are characterized by tests with micro-gravity pendulum bench on ground and with drops in ZARM catapult. The Critical Design Review was achieved successfully on September 2014. The Engineering Model (EM) was integrated and tested successfully, with ground levitation, drops, Electromagnetic Compatibility and thermal vacuum. The integration of the two Flight Models was done on July 2015. The tests will be achieved from July to November 2015. The results of the Engineering Model and Flight Models tests will be presented.

Characteristics of airborne micro-organisms in a neurological intensive care unit: Results from China.

PubMed

Yu, Yao; Yin, Sufeng; Kuan, Yi; Xu, Yingjun; Gao, Xuguang

2015-06-01

To describe the characteristics of airborne micro-organisms in the environment in a Chinese neurological intensive care unit (NICU). This prospective study monitored the air environment in two wards (large and small) of an NICU in a tertiary hospital in China for 12 months, using an LWC-1 centrifugal air sampler. Airborne micro-organisms were identified using standard microbiology techniques. The mean ± SD number of airborne bacteria was significantly higher in the large ward than in the small ward (200 ± 51 colony-forming units [CFU]/m(3) versus 110 ± 40 CFU/m(3), respectively). In the large ward only, the mean number of airborne bacteria in the autumn was significantly higher than in any of the other three seasons. A total of 279 airborne micro-organisms were identified (large ward: 195; small ward: 84). There was no significant difference in the type and distribution of airborne micro-organisms between the large and small wards. The majority of airborne micro-organisms were Gram-positive cocci in both wards. These findings suggest that the number of airborne micro-organisms was related to the number of patients on the NICU ward. © The Author(s) 2015 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Effects of repeated exposure to 4-nonylphenol on the zooplankton community in littoral enclosures

DOE Office of Scientific and Technical Information (OSTI.GOV)

O`Halloran, S.L.; Liber, K.; Gangl, J.A.

1999-03-01

The effects of 4-nonylphenol (NP) on freshwater zooplankton were evaluated in 18 littoral enclosure mesocosms in northeastern Minnesota. The 18 enclosures were allocated to three blocks of six units with each block including two untreated control enclosures and one enclosure for each of four NP treatments. Treated enclosures received 11 applications of NP over a 20-d period between July 8 and 28, 1993. Maximum NP concentrations measured in the water column 2 h after each application averaged ({+-} SD) 5 {+-} 4, 23 {+-} 11, 76 {+-} 21, and 243 {+-} 41 {micro}g/L over the 11 applications. Nonylphenol dissipated rapidlymore » from the water column but was more persistent in sediments and in/on macrophytes. All cladoceran and copepod taxa were significantly reduced in abundance at 243 {+-} 41 {micro}g/L; some sensitive taxa were also affected by 76 {+-} 21 and 23 {+-} 11 {micro}g/L. While many rotifer taxa were unaffected at any of the test concentrations, several were affected at {ge} 76 {+-} 21 {micro}g/L. Ostracods were only affected at 2,243 {+-} 41 {micro}g/L. No zooplankton taxon was affected at 5 {+-} 4 {micro}g/L. The period of maximum impact usually occurred within 1 to 7 d of the last NP application, and recovery to control abundance levels generally occurred within 7 to 28 d of the last NP application. Two sensitive taxa, Acroperus and Calanoida, did not recover at {ge} 76 {+-} 21 {micro}g/L by the end of the study. The maximum acceptable toxicant concentration for protection of all zooplankton taxa was estimated at {approximately} 10 {micro}g/L, although overall community diversity was unaffected at 23 {+-} 11. The water was the most probable route of NP exposure, but the greater persistence of NP residues in/on macrophytes may have contributed to the lack of recovery of some macrophyte-associated taxa.« less

Numerical characterization of micro-cell UO2sbnd Mo pellet for enhanced thermal performance

NASA Astrophysics Data System (ADS)

Lee, Heung Soo; Kim, Dong-Joo; Kim, Sun Woo; Yang, Jae Ho; Koo, Yang-Hyun; Kim, Dong Rip

2016-08-01

Metallic micro-cell UO2 pellet with high thermal conductivity has received attention as a promising accident-tolerant fuel. Although experimental demonstrations have been successful, studies on the potency of current metallic micro-cell UO2 fuels for further enhancement of thermal performance are lacking. Here, we numerically investigated the thermal conductivities of micro-cell UO2sbnd Mo pellets in terms of the amount of Mo content, the unit cell size, and the aspect ratio of the micro-cells. The results showed good agreement with experimental measurements, and more importantly, indicated the importance of optimizing the unit cell geometries of the micro-cell pellets for greater increases in thermal conductivity. Consequently, the micro-cell UO2sbnd Mo pellets (5 vol% Mo) with modified geometries increased the thermal conductivity of the current UO2 pellets by about 2.5 times, and lowered the temperature gradient within the pellets by 62.9% under a linear heat generation rate of 200 W/cm.

Bio-inspired multi-mode optic flow sensors for micro air vehicles

NASA Astrophysics Data System (ADS)

Park, Seokjun; Choi, Jaehyuk; Cho, Jihyun; Yoon, Euisik

2013-06-01

Monitoring wide-field surrounding information is essential for vision-based autonomous navigation in micro-air-vehicles (MAV). Our image-cube (iCube) module, which consists of multiple sensors that are facing different angles in 3-D space, can be applied to the wide-field of view optic flows estimation (μ-Compound eyes) and to attitude control (μ- Ocelli) in the Micro Autonomous Systems and Technology (MAST) platforms. In this paper, we report an analog/digital (A/D) mixed-mode optic-flow sensor, which generates both optic flows and normal images in different modes for μ- Compound eyes and μ-Ocelli applications. The sensor employs a time-stamp based optic flow algorithm which is modified from the conventional EMD (Elementary Motion Detector) algorithm to give an optimum partitioning of hardware blocks in analog and digital domains as well as adequate allocation of pixel-level, column-parallel, and chip-level signal processing. Temporal filtering, which may require huge hardware resources if implemented in digital domain, is remained in a pixel-level analog processing unit. The rest of the blocks, including feature detection and timestamp latching, are implemented using digital circuits in a column-parallel processing unit. Finally, time-stamp information is decoded into velocity from look-up tables, multiplications, and simple subtraction circuits in a chip-level processing unit, thus significantly reducing core digital processing power consumption. In the normal image mode, the sensor generates 8-b digital images using single slope ADCs in the column unit. In the optic flow mode, the sensor estimates 8-b 1-D optic flows from the integrated mixed-mode algorithm core and 2-D optic flows with an external timestamp processing, respectively.

Neural networks for vertical microcode compaction

NASA Astrophysics Data System (ADS)

Chu, Pong P.

1992-09-01

Neural networks provide an alternative way to solve complex optimization problems. Instead of performing a program of instructions sequentially as in a traditional computer, neural network model explores many competing hypotheses simultaneously using its massively parallel net. The paper shows how to use the neural network approach to perform vertical micro-code compaction for a micro-programmed control unit. The compaction procedure includes two basic steps. The first step determines the compatibility classes and the second step selects a minimal subset to cover the control signals. Since the selection process is an NP- complete problem, to find an optimal solution is impractical. In this study, we employ a customized neural network to obtain the minimal subset. We first formalize this problem, and then define an `energy function' and map it to a two-layer fully connected neural network. The modified network has two types of neurons and can always obtain a valid solution.

High density pixel array and laser micro-milling method for fabricating array

NASA Technical Reports Server (NTRS)

McFall, James Earl (Inventor); Wiener-Avnear, Eliezer (Inventor)

2003-01-01

A pixel array device is fabricated by a laser micro-milling method under strict process control conditions. The device has an array of pixels bonded together with an adhesive filling the grooves between adjacent pixels. The array is fabricated by moving a substrate relative to a laser beam of predetermined intensity at a controlled, constant velocity along a predetermined path defining a set of grooves between adjacent pixels so that a predetermined laser flux per unit area is applied to the material, and repeating the movement for a plurality of passes of the laser beam until the grooves are ablated to a desired depth. The substrate is of an ultrasonic transducer material in one example for fabrication of a 2D ultrasonic phase array transducer. A substrate of phosphor material is used to fabricate an X-ray focal plane array detector.

Fabrication of micro metallic valve and pump

NASA Astrophysics Data System (ADS)

Yang, Ming; Kabasawa, Yasunari; Ito, Kuniyoshi

2010-03-01

Fabrication of micro devices by using micro metal forming was proposed by the authors. We developed a desktop servo-press machine with precise tooling system. Precise press forming processes including micro forging and micro joining has been carried out in a progressive die. In this study, micro metallic valve and pump were fabricated by using the precise press forming. The components are made of sheet metals, and assembled in to a unit in the progressive die. A micro check-valve with a diameter of 3mm and a length of 3.2mm was fabricated, and the property of flow resistance was evaluated. The results show that the check valve has high property of leakage proof. Since the valve is a unit parts with dimensions of several millimeters, it has advantage to be adapted to various pump design. Here, two kinds of micro pumps with the check-valves were fabricated. One is diaphragm pump actuated by vibration of the diaphragm, and another is tube-shaped pump actuated by resonation. The flow quantities of the pumps were evaluated and the results show that both of the pumps have high pumping performance.

Emerging Technologies for Assembly of Microscale Hydrogels

PubMed Central

Kavaz, Doga; Demirel, Melik C.; Demirci, Utkan

2013-01-01

Assembly of cell encapsulating building blocks (i.e., microscale hydrogels) has significant applications in areas including regenerative medicine, tissue engineering, and cell-based in vitro assays for pharmaceutical research and drug discovery. Inspired by the repeating functional units observed in native tissues and biological systems (e.g., the lobule in liver, the nephron in kidney), assembly technologies aim to generate complex tissue structures by organizing microscale building blocks. Novel assembly technologies enable fabrication of engineered tissue constructs with controlled properties including tunable microarchitectural and predefined compositional features. Recent advances in micro- and nano-scale technologies have enabled engineering of microgel based three dimensional (3D) constructs. There is a need for high-throughput and scalable methods to assemble microscale units with a complex 3D micro-architecture. Emerging assembly methods include novel technologies based on microfluidics, acoustic and magnetic fields, nanotextured surfaces, and surface tension. In this review, we survey emerging microscale hydrogel assembly methods offering rapid, scalable microgel assembly in 3D, and provide future perspectives and discuss potential applications. PMID:23184717

The trend of digital control system design for nuclear power plants in Korea

DOE Office of Scientific and Technical Information (OSTI.GOV)

Park, S. H.; Jung, H. Y.; Yang, C. Y.

2006-07-01

Currently there are 20 nuclear power plants (NPPs) in operation, and 6 more units are under construction in Korea. The control systems of those NPPs have also been developed together with the technology advancement. Control systems started with On-Off control using the relay logic, had been evolved into Solid-State logic using TTL ICs, and applied with the micro-processors since the Yonggwang NPP Units 3 and 4 which started its construction in 1989. Multiplexers are also installed at the local plant areas to collect field input and to send output signals while communicating with the controllers located in the system cabinetsmore » near the main control room in order to reduce the field wiring cables. The design of the digital control system technology for the NPPs in Korea has been optimized to maximize the operability as well as the safety through the design, construction, start-up and operation experiences. Both Shin-Kori Units 1 and 2 and Shin-Wolsong Units 1 and 2 NPP projects under construction are being progressed at the same time. Digital Plant Control Systems of these projects have adopted multi-loop controllers, redundant loop configuration, and soft control system for the radwaste system. Programmable Logic Controller (PLC) and Distributed Control System (DCS) are applied with soft control system in Shin-Kori Units 3 and 4. This paper describes the evolvement of control system at the NPPs in Korea and the experience and design improvement through the observation of the latest failure of the digital control system. In addition, design concept and its trend of the digital control system being applied to the NPP in Korea are introduced. (authors)« less

Design and validation of a low cost surface plasmon resonance bioanalyzer using microprocessors and a touch-screen monitor.

PubMed

Hu, Jiandong; Hu, Jingfang; Luo, Fukun; Li, Wei; Jiang, Guoliang; Li, Zhengfeng; Zhang, Runna

2009-03-15

An economical and high-performance bioanalyzer, with no use of laptop computer, based on the use of TSPR1k23 biosensors was systematically designed, and validated experimentally for its high performance. The analyzer is composed of a micro-flow cell, a thermoelectric cooler (TEC), a clamp, a touch-screen monitor, and an electronic control unit (ECU) incorporated with photoelectric conversion device. The micro-flow cell is made of stainless steel with high thermal conductivity, and the micro-flow system is based on PID temperature-controlled algorithm to keep the constant temperature (25 degrees C) of the liquid sample via thermal exchange with the clamp. With a peristaltic pump implemented by an injection loop flow system, the bioanalyzer allows the core sensor to be completely exposed to samples. The touch-screen monitor displays the normalized response signal values updated every 0.25s, with a typical noise level less than 5RU (response unit) within 2h. The bioanalyzer was validated using hepatitis B surface antigen (HBsAg) as an example. Anti-HBsAg monoclonal antibody is adhered to the surface of the sensor chip by a bifunctional cross-linker with the technology of self-assembly. The duration of the HBsAg measurement only lasts 5min with a dilution factor ranging from 200 to 1200, optimized with a R-squared value 0.998. The results suggested that the bioanalyzer has higher selectivity, lower cost, expanded detection limit, and shorter measuring time as compared with the HBsAg ELISA kit, especially for low concentrations of analyte.

A superior architecture of brightness enhancement for display backlighting

NASA Astrophysics Data System (ADS)

Dross, Oliver; Parkyn, William A.; Chaves, Julio; Falicoff, Waqidi; Miñano, Juan Carlos; Benitez, Pablo; Alvarez, Roberto

2006-08-01

Brightness enhancement of backlighting for displays is typically achieved via crossed micro prismatic films that are introduced between a backlight unit and a transmissive (LCD) display. Prismatic films let pass light only into a restricted angular range, while, in conjunction with other reflective elements below the backlight, all other light is recycled within the backlight unit, thereby increasing the backlight luminance. This design offers no free parameters to influence the resulting light distribution and suffers from insufficient stray light control. A novel strategy of light recycling is introduced, using a microlens array in conjunction with a hole array in a reflective surface, that can provide higher luminance, superior stray light control, and can be designed to meet almost any desired emission pattern. Similar strategies can be applied to mix light from different colored LEDs being mounted upside down to shine into a backlight unit.

In Vitro Comparative Study of Two Different Bleaching Agents on Micro-hardness Dental Enamel.

PubMed

Fatima, Nazish; Ali Abidi, Syed Yawar; Meo, Ashraf Ali

2016-02-01

To evaluate the effect of home-use bleaching agent containing 16% Carbamide Peroxide (CP) and in-office bleaching agent containing 38% Hydrogen Peroxide (HP) on enamel micro-hardness. An in vitroexperimental study. Department of Operative Dentistry and Science of Dental Materials at Dr. Ishrat-ul-Ebad Khan Institute of Oral Health Sciences, Dow University of Health Sciences and Material Engineering Department of NED University of Engineering and Technology, Karachi, from July to December 2014. Atotal of 90 enamel slabs from 45 sound human 3rd molar were randomly divided into 3 groups. Each group contained 30 specimens (n=30). Group 1 was kept in artificial saliva at 37°C in incubator during the whole experiment. However, Groups 2 and 3 were treated with power whitening gel and tooth whitening pen respectively. After bleaching session, specimens were thoroughly rinsed with deionized water again for 10 seconds and then stored in artificial saliva at 37°C in incubator. Artificial saliva was changed after every 2 days. The Vickers hardness tester (Wolpert 402 MVD, Germany) was adjusted to a load of 0.1 kg (100 gm) and dwell time of 5 seconds. Three Vickers were performed on each specimen using a hardness tester according to the ISO 6507-3:1998 specification. Micro-hardness measurements were performed before and after bleaching at day 1, 7 and 14. In the control group, the baseline micro-hardness was 181.1 ±9.3 which was reduced after the storage on day 1, 7 and 14 (p = 0.104). In Group 2, baseline micro-hardness was 180.4 ±10.1 which was reduced to 179.79 ±10.0 units after day 1. Whereas, on day 7 and 14, the values of micro-hardness were 179.8 ±10 and 179.7 ±10.29, respectively (p=0.091). Furthermore, the baseline micro-hardness in Group 3 was 174.0 ±22.9 units which was reduced to 173 ±23 on day 1, 170 ±30 on day 7 and 173 ±23 on day 14 (p = 0.256). The statistically insignificant difference was found among micro-hardness values of different bleaching agents (p = 0.118). Bleaching with 38% Hydrogen Peroxide (HP) and 16% Carbamide Peroxide (CP) resulted in insignificant effect on surface micro-hardness of enamel.

A robust adaptive load frequency control for micro-grids.

PubMed

Khooban, Mohammad-Hassan; Niknam, Taher; Blaabjerg, Frede; Davari, Pooya; Dragicevic, Tomislav

2016-11-01

The goal of this study is to introduce a novel robust load frequency control (LFC) strategy for micro-grid(s) (MG(s)) in islanded mode operation. Admittedly, power generators in MG(s) cannot supply steady electric power output and sometimes cause unbalance between supply and demand. Battery energy storage system (BESS) is one of the effective solutions to these problems. Due to the high cost of the BESS, a new idea of Vehicle-to-Grid (V2G) is that a battery of Electric-Vehicle (EV) can be applied as a tantamount large-scale BESS in MG(s). As a result, a new robust control strategy for an islanded micro-grid (MG) is introduced that can consider electric vehicles׳ (EV(s)) effect. Moreover, in this paper, a new combination of the General Type II Fuzzy Logic Sets (GT2FLS) and the Modified Harmony Search Algorithm (MHSA) technique is applied for adaptive tuning of proportional-integral (PI) controller. Implementing General Type II Fuzzy Systems is computationally expensive. However, using a recently introduced α-plane representation, GT2FLS can be seen as a composition of several Interval Type II Fuzzy Logic Systems (IT2FLS) with a corresponding level of α for each. Real-data from an offshore wind farm in Sweden and solar radiation data in Aberdeen (United Kingdom) was used in order to examine the performance of the proposed novel controller. A comparison is made between the achieved results of Optimal Fuzzy-PI (OFPI) controller and those of Optimal Interval Type II Fuzzy-PI (IT2FPI) controller, which are of most recent advances in the area at hand. The Simulation results prove the successfulness and effectiveness of the proposed controller. Copyright © 2016 ISA. Published by Elsevier Ltd. All rights reserved.

Mechanics of additively manufactured porous biomaterials based on the rhombicuboctahedron unit cell.

PubMed

Hedayati, R; Sadighi, M; Mohammadi-Aghdam, M; Zadpoor, A A

2016-01-01

Thanks to recent developments in additive manufacturing techniques, it is now possible to fabricate porous biomaterials with arbitrarily complex micro-architectures. Micro-architectures of such biomaterials determine their physical and biological properties, meaning that one could potentially improve the performance of such biomaterials through rational design of micro-architecture. The relationship between the micro-architecture of porous biomaterials and their physical and biological properties has therefore received increasing attention recently. In this paper, we studied the mechanical properties of porous biomaterials made from a relatively unexplored unit cell, namely rhombicuboctahedron. We derived analytical relationships that relate the micro-architecture of such porous biomaterials, i.e. the dimensions of the rhombicuboctahedron unit cell, to their elastic modulus, Poisson's ratio, and yield stress. Finite element models were also developed to validate the analytical solutions. Analytical and numerical results were compared with experimental data from one of our recent studies. It was found that analytical solutions and numerical results show a very good agreement particularly for smaller values of apparent density. The elastic moduli predicted by analytical and numerical models were in very good agreement with experimental observations too. While in excellent agreement with each other, analytical and numerical models somewhat over-predicted the yield stress of the porous structures as compared to experimental data. As the ratio of the vertical struts to the inclined struts, α, approaches zero and infinity, the rhombicuboctahedron unit cell respectively approaches the octahedron (or truncated cube) and cube unit cells. For those limits, the analytical solutions presented here were found to approach the analytic solutions obtained for the octahedron, truncated cube, and cube unit cells, meaning that the presented solutions are generalizations of the analytical solutions obtained for several other types of porous biomaterials. Copyright © 2015 Elsevier Ltd. All rights reserved.

Spatiotemporal historical datasets at micro-level for geocoded individuals in five Swedish parishes, 1813–1914

PubMed Central

Hedefalk, Finn; Svensson, Patrick; Harrie, Lars

2017-01-01

This paper presents datasets that enable historical longitudinal studies of micro-level geographic factors in a rural setting. These types of datasets are new, as historical demography studies have generally failed to properly include the micro-level geographic factors. Our datasets describe the geography over five Swedish rural parishes, and by linking them to a longitudinal demographic database, we obtain a geocoded population (at the property unit level) for this area for the period 1813–1914. The population is a subset of the Scanian Economic Demographic Database (SEDD). The geographic information includes the following feature types: property units, wetlands, buildings, roads and railroads. The property units and wetlands are stored in object-lifeline time representations (information about creation, changes and ends of objects are recorded in time), whereas the other feature types are stored as snapshots in time. Thus, the datasets present one of the first opportunities to study historical spatio-temporal patterns at the micro-level. PMID:28398288

Microprocessor-based integration of microfluidic control for the implementation of automated sensor monitoring and multithreaded optimization algorithms.

PubMed

Ezra, Elishai; Maor, Idan; Bavli, Danny; Shalom, Itai; Levy, Gahl; Prill, Sebastian; Jaeger, Magnus S; Nahmias, Yaakov

2015-08-01

Microfluidic applications range from combinatorial synthesis to high throughput screening, with platforms integrating analog perfusion components, digitally controlled micro-valves and a range of sensors that demand a variety of communication protocols. Currently, discrete control units are used to regulate and monitor each component, resulting in scattered control interfaces that limit data integration and synchronization. Here, we present a microprocessor-based control unit, utilizing the MS Gadgeteer open framework that integrates all aspects of microfluidics through a high-current electronic circuit that supports and synchronizes digital and analog signals for perfusion components, pressure elements, and arbitrary sensor communication protocols using a plug-and-play interface. The control unit supports an integrated touch screen and TCP/IP interface that provides local and remote control of flow and data acquisition. To establish the ability of our control unit to integrate and synchronize complex microfluidic circuits we developed an equi-pressure combinatorial mixer. We demonstrate the generation of complex perfusion sequences, allowing the automated sampling, washing, and calibrating of an electrochemical lactate sensor continuously monitoring hepatocyte viability following exposure to the pesticide rotenone. Importantly, integration of an optical sensor allowed us to implement automated optimization protocols that require different computational challenges including: prioritized data structures in a genetic algorithm, distributed computational efforts in multiple-hill climbing searches and real-time realization of probabilistic models in simulated annealing. Our system offers a comprehensive solution for establishing optimization protocols and perfusion sequences in complex microfluidic circuits.

Exploring the use of routinely-available, retrospective data to study the association between malaria control scale-up and micro-economic outcomes in Zambia.

PubMed

Comfort, Alison; Leegwater, Anthony; Nakhimovsky, Sharon; Kansembe, Henry; Hamainza, Busiku; Bwalya, Benson; Alilio, Martin; Johns, Ben; Olsho, Lauren

2017-01-04

Country-level evidence on the impact of malaria control on micro-economic outcomes is vital for mobilizing domestic and donor resources for malaria control. Using routinely available survey data could facilitate this investigation in a cost-efficient way. The authors used Malaria Indicator Surveys (MIS) and Living Conditions Monitoring Survey (LCMS) data from 2006 to 2010 for all 72 districts in Zambia to relate malaria control scale-up with household food spending (proxy for household well-being), educational attainment and agricultural production. The authors used two quasi-experimental designs: (1) a generalized propensity score for a continuous treatment variable (defined as coverage from owning insecticide-treated bed nets and/or receipt of indoor residual spraying); and, (2) a district fixed effects model to assess changes in the outcome relative to changes in treatment pre-post scale-up. The unit of analysis was at district level. The authors also conducted simulations post-analysis to assess statistical power. Micro-economic outcomes increased (33% increase in food spending) concurrently with malaria control coverage (62% increase) from 2006 to 2010. Despite using data from all 72 districts, both analytic methods yielded wide confidence intervals that do not conclusively link outcomes and malaria control coverage increases. The authors cannot rule out positive, null or negative effects. The upper bound estimates of the results show that if malaria control coverage increases from 60 to 70%, food spending could increase up to 14%, maize production could increase up to 57%, and years of schooling could increase up to 0.5 years. Simulations indicated that the generalized propensity score model did not have good statistical power. While it is technically possible to use routinely available survey data to relate malaria control scale-up and micro-economic outcomes, it is not clear from this analysis that meaningful results can be obtained when survey data are highly aggregated. Researchers in similar settings should assess the feasibility of disaggregating existing survey data. Additionally, large surveys, such as LCMS and MIS, could incorporate data on both malaria coverage and household expenditures, respectively.

Multi-terminal remote monitoring and warning system using Micro Air Vehicle for dangerous environment

NASA Astrophysics Data System (ADS)

Yu, Yanan; Wang, Xiaoxun; He, Chengcheng; Lai, Chenlong; Liu, Yuanchao

2015-11-01

For overcoming the problems such as remote operation and dangerous tasks, multi-terminal remote monitoring and warning system based on STC89C52 Micro Control Unit and wireless communication technique was proposed. The system with MCU as its core adopted multiple sets of sensor device to monitor environment parameters of different locations, such as temperature, humidity, smoke other harmful gas concentration. Data information collected was transmitted remotely by wireless transceiver module, and then multi-channel data parameter was processed and displayed through serial communication protocol between the module and PC. The results of system could be checked in the form of web pages within a local network which plays a wireless monitoring and warning role. In a remote operation, four-rotor micro air vehicle which fixed airborne data acquisition device was utilized as a middleware between collecting terminal and PC to increase monitoring scope. Whole test system has characteristics of simple construction, convenience, real time ability and high reliability, which could meet the requirements of actual use.

MAGI: a Node.js web service for fast microRNA-Seq analysis in a GPU infrastructure.

PubMed

Kim, Jihoon; Levy, Eric; Ferbrache, Alex; Stepanowsky, Petra; Farcas, Claudiu; Wang, Shuang; Brunner, Stefan; Bath, Tyler; Wu, Yuan; Ohno-Machado, Lucila

2014-10-01

MAGI is a web service for fast MicroRNA-Seq data analysis in a graphics processing unit (GPU) infrastructure. Using just a browser, users have access to results as web reports in just a few hours->600% end-to-end performance improvement over state of the art. MAGI's salient features are (i) transfer of large input files in native FASTA with Qualities (FASTQ) format through drag-and-drop operations, (ii) rapid prediction of microRNA target genes leveraging parallel computing with GPU devices, (iii) all-in-one analytics with novel feature extraction, statistical test for differential expression and diagnostic plot generation for quality control and (iv) interactive visualization and exploration of results in web reports that are readily available for publication. MAGI relies on the Node.js JavaScript framework, along with NVIDIA CUDA C, PHP: Hypertext Preprocessor (PHP), Perl and R. It is freely available at http://magi.ucsd.edu. © The Author 2014. Published by Oxford University Press.

Around Marshall

NASA Image and Video Library

2004-02-01

Andy Jenkins, an engineer for the Lab on a Chip Applications Development program, helped build the Applications Development Unit (ADU-25), a one-of-a-kind facility for controlling and analyzing processes on chips with extreme accuracy. Pressure is used to cause fluids to travel through network of fluid pathways, or micro-channels, embossed on the chips through a process similar to the one used to print circuits on computer chips. To make customized chips for various applications, NASA has an agreement with the U.S. Army's Micro devices and Micro fabrication Laboratory at Redstone Arsenal in Huntsville, Alabama, where NASA's Marshall Space Flight Center (MSFC) is located. The Marshall Center team is also collaborating with scientists at other NASA centers and at universities to develop custom chip designs for many applications, such as studying how fluidic systems work in spacecraft and identifying microbes in self-contained life support systems. Chips could even be designed for use on Earth, such as for detecting deadly microbes in heating and air systems. (NASA/MSFC/D.Stoffer)

MicroRNA-155 regulates host immune response to postviral bacterial pneumonia via IL-23/IL-17 pathway.

PubMed

Podsiad, Amy; Standiford, Theodore J; Ballinger, Megan N; Eakin, Richard; Park, Pauline; Kunkel, Steven L; Moore, Bethany B; Bhan, Urvashi

2016-03-01

Postinfluenza bacterial pneumonia is associated with significant mortality and morbidity. MicroRNAs (miRNAs) are small, noncoding RNAs that regulate gene expression posttranscriptionally. miR-155 has recently emerged as a crucial regulator of innate immunity and inflammatory responses and is induced in macrophages during infection. We hypothesized upregulation of miR-155 inhibits IL-17 and increases susceptibility to secondary bacterial pneumonia. Mice were challenged with 100 plaque-forming units H1N1 intranasally and were infected with 10(7) colony-forming units of MRSA intratracheally at day 5 postviral challenge. Lungs were harvested 24 h later, and expression of miR-155, IL-17, and IL-23 was measured by real-time RT-PCR. Induction of miR-155 was 3.6-fold higher in dual-infected lungs compared with single infection. miR-155(-/-) mice were protected with significantly lower (4-fold) bacterial burden and no differences in viral load, associated with robust induction of IL-23 and IL-17 (2.2- and 4.8-fold, respectively) postsequential challenge with virus and bacteria, compared with WT mice. Treatment with miR-155 antagomir improved lung bacterial clearance by 4.2-fold compared with control antagomir postsequential infection with virus and bacteria. Moreover, lung macrophages collected from patients with postviral bacterial pneumonia also had upregulation of miR-155 expression compared with healthy controls, consistent with observations in our murine model. This is the first demonstration that cellular miRNAs regulate postinfluenza immune response to subsequent bacterial challenge by suppressing the IL-17 pathway in the lung. Our findings suggest that antagonizing certain microRNA might serve as a potential therapeutic strategy against secondary bacterial infection. Copyright © 2016 the American Physiological Society.

Dynamic Control System Mode Performance of the Space Technology-7 Disturbance Reduction System

NASA Technical Reports Server (NTRS)

O'Donnell, James R., Jr.; Hsu, Oscar; Maghami, Peiman

2017-01-01

The Space Technology-7 (ST-7) Disturbance Reduction System (DRS) is an experiment package aboard the European Space Agency (ESA) LISA Pathfinder spacecraft, launched on December 3, 2015. DRS consists of three primary components: Colloidal MicroNewton Thrusters (CMNTs), an Integrated Avionics Unit (IAU), and flight-software implementing the Command and Data Handling (C&DH) and Dynamic Control System (DCS) algorithms. The CMNTs were designed to provide thrust from 5 to 30 micro Newton, with thrust controllability and resolution of 0.1 micro Newton and thrust noise of 0.1 micro Newton/(square root of (Hz)) in the measurement band from 1-30 mHz. The IAU hosts the C&DH and DCS flight software, as well as interfaces with both the CMNT electronics and the LISA Pathfinder spacecraft. When in control, the DCS uses star tracker attitude data and capacitive or optically-measured position and attitude information from LISA Pathfinder and the LISA Technology Package (LTP) to control the attitude and position of the spacecraft and the two test masses inside the LTP. After completion of the nominal ESA LISA Pathfinder mission, the DRS experiment was commissioned followed by its nominal mission. DRS operations extended over the next five months, interspersed with station keeping, anomaly resolution, and periods where control was handed back to LISA Pathfinder for them to conduct further experiments. The primary DRS mission ended on December 6, 2016, with the experiment meeting all of its Level 1 requirements. The DCS, developed at the NASA Goddard Space Flight Center, consists of five spacecraft control modes and six test mass control modes, combined into six 'DRS Mission Modes'. Attitude Control and Zero-G were primarily used to control the spacecraft during initial handover and during many of the CMNT characterization experiments. The other Mission Modes, Drag Free Low Force, 18-DOF Transitional, and 18-DOF, were used to provide drag-free control of the spacecraft about the test masses. This paper will discuss the performance of these DCS spacecraft and test mass control modes. Flight data will be shown from each mode throughout the mission, both from nominal operations and during various flight experiments. The DCS team also made some changes to controller, filter, and limit parameters during operations; the motivation and results of these changes will be shown and discussed.

Status of Electrostatic Accelerometer Development for Gravity Recovery and Climate Experiment Follow-on Mission (GRACE FO)

NASA Astrophysics Data System (ADS)

Lebat, V.; Boulanger, D.; Christophe, B.; Foulon, B.; Liorzou, F.; Perrot, E.; Huynh, P. A.

2014-12-01

The GRACE FO mission, led by the JPL (Jet Propulsion Laboratory), is an Earth-orbiting gravity mission, continuation of the GRACE mission, which will produce an accurate model of the Earth's gravity field variation providing global climatic data during five years at least. The mission involves two satellites in a loosely controlled tandem formation, with a micro-wave link measuring the inter-satellites distance variation. Earth's mass distribution non-uniformities cause variations of the inter-satellite distance. This variation is measured to recover gravity, after subtracting the non-gravitational contributors, as the residual drag. ONERA (the French Aerospace Lab) is developing, manufacturing and testing electrostatic accelerometers measuring this residual drag applied on the satellites. The accelerometer is composed of two main parts: the Sensor Unit (including the Sensor Unit Mechanics - SUM - and the Front-End Electronic Unit - FEEU) and the Interface Control Unit - ICU. In the Accelerometer Core, located in the Sensor Unit Mechanics, the proof mass is levitated and maintained at the center of an electrode cage by electrostatic forces. Thus, any drag acceleration applied on the satellite involves a variation on the servo-controlled electrostatic suspension of the mass. The voltage on the electrodes providing this electrostatic force is the measurement output of the accelerometer. The impact of the accelerometer defaults (geometry, electronic and parasitic forces) leads to bias, misalignment and scale factor error, non-linearity and noise. Some of these accelerometer defaults are characterized by tests with micro-gravity pendulum bench on ground and with drops in ZARM catapult. The Preliminary Design Review was achieved successfully on November 2013. The Engineering Model (EM) was integrated successfully and is under test, with ground levitation, drops, Electromagnetic Compatibility and thermal vacuum. The complete EM tests will be achieved on October 2014. The Critical Design Review is scheduled at the end of September 2014, and the integration of the first Flight Model will begin on October 2014. The results of the Engineering Model tests and the status of the Flight Models will be presented.

Micro-Structured Two-Component 3D Metamaterials with Negative Thermal-Expansion Coefficient from Positive Constituents

PubMed Central

Qu, Jingyuan; Kadic, Muamer; Naber, Andreas; Wegener, Martin

2017-01-01

Controlling the thermal expansion of materials is of great technological importance. Uncontrolled thermal expansion can lead to failure or irreversible destruction of structures and devices. In ordinary crystals, thermal expansion is governed by the asymmetry of the microscopic binding potential, which cannot be adjusted easily. In artificial crystals called metamaterials, thermal expansion can be controlled by structure. Here, following previous theoretical work, we fabricate three-dimensional (3D) two-component polymer micro-lattices by using gray-tone laser lithography. We perform cross-correlation analysis of optical microscopy images taken at different sample temperatures. The derived displacement-vector field reveals that the thermal expansion and resulting bending of the bi-material beams leads to a rotation of the 3D chiral crosses arranged onto a 3D checkerboard pattern within one metamaterial unit cell. These rotations can compensate the expansion of the all positive constituents, leading to an effectively near-zero thermal length-expansion coefficient, or over-compensate the expansion, leading to an effectively negative thermal length-expansion coefficient. This evidences a striking level of thermal-expansion control. PMID:28079161

Ultra-Low Power Event-Driven Wireless Sensor Node Using Piezoelectric Accelerometer for Health Monitoring

NASA Astrophysics Data System (ADS)

Okada, Hironao; Kobayashi, Takeshi; Masuda, Takashi; Itoh, Toshihiro

2009-07-01

We describe a low power consumption wireless sensor node designed for monitoring the conditions of animals, especially of chickens. The node detects variations in 24-h behavior patterns by acquiring the number of the movement of an animal whose acceleration exceeds a threshold measured in per unit time. Wireless sensor nodes when operated intermittently are likely to miss necessary data during their sleep mode state and waste the power in the case of acquiring useless data. We design the node worked only when required acceleration is detected using a piezoelectric accelerometer and a comparator for wake-up source of micro controller unit.

Diode step stress program for JANTX1N5615

NASA Technical Reports Server (NTRS)

1979-01-01

The effect of power/temperature step stress when applied to the switching diode JANTX1N5615 manufactured by Semtech and Micro semiconductor was examined. A total of 48 samples from each manufacturer were submitted to the process. In addition, two control sample units were maintained for verification of the electrical parametric testing. All test samples were subjected to the electrical tests after completing the prior power/temperature step stress point. Results are presented.

United States Air Force Research Initiation Program for 1988. Volume 2

DTIC Science & Technology

1990-04-01

Specialty: Modeling and Simulation ENGINEERING AND SERVICES CENTER (Tyndall Air Force Base) Dr. Wayne A. Charlie Dr. Peter Jeffers (1987) Colorado State...Michael Sydor University of New Hampshire University of Minnesota Specialty: Systems Modeling & Controls Specialty: Optics, Material Science Dr. John...9MG-025 4 Modeling and Simulation on Micro- Dr. Joseph J. Feeley (1987) computers, 1989 760-7MG-070 5 Two Dimensional MHD Simulation of Dr. Manuel A

Controlling measures of micro-plastic and nano pollutants: A short review of disposing waste toners.

PubMed

Ruan, Jujun; Qin, Baojia; Huang, Jiaxin

2018-05-31

Micro-plastic and nano-particle have been the focal pollutants in environmental science. The printer toner is omitted micro-plastic and nano pollutant. It is comprised of micro polyacrylate styrene and nano-Fe 3 O 4 particles. Polyacrylate styrene and nano-metal were proved to be irreversibly toxic to biological cells. Therefore, toners have the potential environmental risk and healthy harm due to include micro plastics and nano-metal. To our knowledge, few studies provided the specific collection and treatment of micro-plastic pollutant. This paper has chosen a kind of micro-plastic and nano pollutant toxic toner and provided technical guidance and inspiration for controlling the micro-plastic and nano pollutants. The method of vacuum-gasification-condensation was adopted for controlling the micro-plastic and nano pollutant toner. We believe this review will open up a potential avenue for controlling micro-plastic and nano pollutants for environmental protection. Copyright © 2018 Elsevier Ltd. All rights reserved.

Studying neighborhood crime across different macro spatial scales: The case of robbery in 4 cities.

PubMed

Hipp, John R; Wo, James C; Kim, Young-An

2017-11-01

Whereas there is a burgeoning literature focusing on the spatial distribution of crime events across neighborhoods or micro-geographic units in a specific city, the present study expands this line of research by selecting four cities that vary across two macro-spatial dimensions: population in the micro-environment, and population in the broader macro-environment. We assess the relationship between measures constructed at different spatial scales and robbery rates in blocks in four cities: 1) San Francisco (high in micro- and macro-environment population); 2) Honolulu (high in micro- but low in macro-environment population); 3) Los Angeles (low in micro- but high in macro-environment population); 4) Sacramento (low in micro- and macro-environment population). Whereas the socio-demographic characteristics of residents further than ½ mile away do not impact robbery rates, the number of people up to 2.5 miles away are related to robbery rates, especially in the two cities with smaller micro-environment population, implying a larger spatial scale than is often considered. The results show that coefficient estimates differ somewhat more between cities differing in micro-environment population compared to those differing based on macro-environment population. It is therefore necessary to consider the broader macro-environment even when focusing on the level of crime across neighborhoods or micro-geographic units within an area. Copyright © 2017 Elsevier Inc. All rights reserved.

Status Report on the Development of Micro-Scheduling Software for the Advanced Outage Control Center Project

DOE Office of Scientific and Technical Information (OSTI.GOV)

Germain, Shawn St.; Thomas, Kenneth; Farris, Ronald

2014-09-01

The long-term viability of existing nuclear power plants (NPPs) in the United States (U.S.) is dependent upon a number of factors, including maintaining high capacity factors, maintaining nuclear safety, and reducing operating costs, particularly those associated with refueling outages. Refueling outages typically take 20-30 days, and for existing light water NPPs in the U.S., the reactor cannot be in operation during the outage. Furthermore, given that many NPPs generate between $1-1.5 million/day in revenue when in operation, there is considerable interest in shortening the length of refueling outages. Yet, refueling outages are highly complex operations, involving multiple concurrent and dependentmore » activities that are difficult to coordinate. Finding ways to improve refueling outage performance while maintaining nuclear safety has proven to be difficult. The Advanced Outage Control Center project is a research and development (R&D) demonstration activity under the Light Water Reactor Sustainability (LWRS) Program. LWRS is a R&D program which works with industry R&D programs to establish technical foundations for the licensing and managing of long-term, safe, and economical operation of current NPPs. The Advanced Outage Control Center project has the goal of improving the management of commercial NPP refueling outages. To accomplish this goal, this INL R&D project is developing an advanced outage control center (OCC) that is specifically designed to maximize the usefulness of communication and collaboration technologies for outage coordination and problem resolution activities. This report describes specific recent efforts to develop a capability called outage Micro-Scheduling. Micro-Scheduling is the ability to allocate and schedule outage support task resources on a sub-hour basis. Micro-Scheduling is the real-time fine-tuning of the outage schedule to react to the actual progress of the primary outage activities to ensure that support task resources are optimally deployed with the least amount of delay and unproductive use of resources. The remaining sections of this report describe in more detail the scheduling challenges that occur during outages, how a Micro-Scheduling capability helps address those challenges, and provides a status update on work accomplished to date and the path forward.« less

Development of dissolved air flotation technology from the first generation to the newest (third) one (DAF in turbulent flow conditions).

PubMed

Kiuru, H J

2001-01-01

This paper gives a brief description of the development of dissolved air flotation DAF (or so-called high pressure flotation) as an unit operation for removal of solids in water and wastewater treatment during the last 80 years up to this time. The first DAF-systems used in the water industry were the ADKA and Sveen-Pedersen ones from the 1920s. Some of these are still in use. The tanks in which the flotation phenomenon takes place in these systems are very shallow and narrow as well as rather long. The flow rate of water is some 2-3 m/h (at most less than 5 m/h only) and there is a very thin micro-bubble blanket below the water surface between the dry sludge blanket on that and the clarified water which flows almost horizontally below the bubble blanket toward the end of the tanks to be taken out there from near the bottom. The second generation of DAF was introduced in the 1960s and these units are widely in use today. Their tanks are almost square ones having usually a little bit more length than breadth. They are rather deep, too. There is an under-flow wall in front of the back wall of the units having a narrow horizontal gap on the bottom of the tanks for letting out the clarified water from the flotation space. The flow rate of water is usually 5-7 m/h or at most less than 10 m/h. The direction of flow is 30-45 degrees below the horizontal. There is a rather thick micro-bubble bed at the beginning of the tank below the dry sludge blanket. This bubble-bed becomes clearly thinner, when going toward the end of the tank. There are also round DAF tanks which are based on the same hydraulic principles as the rectangular ones presented above. A special application of DAF called the flotation filter was invented at the very end of the 1960s. It is a combination of flotation and rapid sand filtration, both of those being placed in the same tank. Flotation takes place in the upper part of the tank and the filter has been placed in the lower part of it. The direction of water flow is now vertically down from the free surface of water in the tank toward the deep-bed filter. This controls the direction of flow in the flotation space of the tank above the filter bed. The flow rate of water in flotation filters may be 10-15 m/h, but the flow conditions are still laminar. It is the threat that the head-loss of filters would grow too rapidly which in practice is limiting the hydraulic flow rate of flotation filters in this area. The third generation of DAF has been developed at the end of the 1990s. The operational idea is based on that of the flotation filter. The filter bed on the bottom of the tank has been replaced by a thin stiff plate with plenty of round orifices throughout the plate. This plate, having a very much lower flowing resistance than a sand filter can have, controls the vertical flow of water in the flotation space above the plate and distributes it evenly throughout the horizontal cross-section of the tank. The flotation tank is almost square seen from above and its depth is clearly more than the length and breadth of it. This kind of flotation unit can be operated with flow rates of water in the range 25-40 m/l. Even a flow rate of more than 60 m/h has been reported from this kind of DAF-units. There is no risk of clogging of the plate by suspended solids which could limit the flow rate. This is to say that it is possible to operate DAF also in turbulent flow conditions. The depth of the micro-bubble bed below the surface of water can be 1.5-2.5 m. There actually is a continuously regenerated micro-bubble bed in the tank filtering water which is going through this bed. The lower surface of the micro-bubble bed is really a horizontal one a little bit above the plate controlling the flow in the flotation space. The clarified water below the micro-bubble bed is totally clear. It can be said that in this case the removal of suspended solids takes place much more by filtering water by a deep-bed micro-bubble filter than by attaching micro-bubbles onto solids, when both of these are mixed with each other in the inlet shaft of the flotation unit, because the retention time of water in the inlet shaft is very short indeed.

Design and simulation of flight control system for man-portable micro reconnaissance quadcopter

NASA Astrophysics Data System (ADS)

Yin, Xinfan; Zhang, Daibing; Fang, Qiang; Shen, Lincheng

2017-10-01

The quadcopter has been widely used in the field of aerial photography and environmental detection, because of its advantages of VTOL, simple structure, and easy-control. In the field of urban anti-terrorism or special operations, micro reconnaissance quadcpter has its unique advantages such as all-weather taking off and landing, small noise and so on, and it is very popular with special forces and riot police. This paper aims at the flight control problem of the micro quadcopter, for the purposes of attitude stabilization control and trajectory tracking control of the micro quadcopter, first, the modeling of the micro quadcopter is presented. And using the MATLAB/SIMULINK toolbox to build the flight controller of the micro quadcopter, and then simulation analysis and real flight test are given. The results of the experiment show that the designed PID controller can correct the flight attitude shift effectively and track the planned tracks well, and can achieve the goal of stable and reliable flight of the quadcopter. It can be a useful reference for the flight control system design of future special operations micro UAV.

Active Fail-Safe Micro-Array Flow Control for Advanced Embedded Propulsion Systems

NASA Technical Reports Server (NTRS)

Anderson, Bernhard H.; Mace, James L.; Mani, Mori

2009-01-01

The primary objective of this research effort was to develop and analytically demonstrate enhanced first generation active "fail-safe" hybrid flow-control techniques to simultaneously manage the boundary layer on the vehicle fore-body and to control the secondary flow generated within modern serpentine or embedded inlet S-duct configurations. The enhanced first-generation technique focused on both micro-vanes and micro-ramps highly-integrated with micro -jets to provide nonlinear augmentation for the "strength' or effectiveness of highly-integrated flow control systems. The study focused on the micro -jet mass flow ratio (Wjet/Waip) range from 0.10 to 0.30 percent and jet total pressure ratios (Pjet/Po) from 1.0 to 3.0. The engine bleed airflow range under study represents about a 10 fold decrease in micro -jet airflow than previously required. Therefore, by pre-conditioning, or injecting a very small amount of high-pressure jet flow into the vortex generated by the micro-vane and/or micro-ramp, active flow control is achieved and substantial augmentation of the controlling flow is realized.

Total integrated slidable and valveless solid phase extraction-polymerase chain reaction-capillary electrophoresis microdevice for mini Y chromosome short tandem repeat genotyping.

PubMed

Kim, Yong Tae; Lee, Dohwan; Heo, Hyun Young; Sim, Jeong Eun; Woo, Kwang Man; Kim, Do Hyun; Im, Sung Gap; Seo, Tae Seok

2016-04-15

A fully integrated slidable and valveless microsystem, which performs solid phase DNA extraction (SPE), micro-polymerase chain reaction (μPCR) and micro-capillary electrophoresis (μCE) coupled with a portable genetic analyser, has been developed for forensic genotyping. The use of a slidable chip, in which a 1 μL-volume of the PCR chamber was patterned at the center, does not necessitate any microvalves and tubing systems for fluidic control. The functional micro-units of SPE, μPCR, and μCE were fabricated on a single glass wafer by conventional photolithography, and the integrated microdevice consists of three layers: from top to bottom, a slidable chip, a channel wafer in which a SPE chamber, a mixing microchannel, and a CE microchannel were fabricated, and a Ti/Pt resistance temperature detector (RTD) wafer. The channel glass wafer and the RTD glass wafer were thermally bonded, and the slidable chip was placed on the designated functional unit. The entire process from the DNA extraction using whole human blood sample to identification of target Y chromosomal short tandem repeat (STR) loci was serially carried out with simply sliding the slidable chamber from one to another functional unit. Monoplex and multiplex detection of amelogenin and mini Y STR loci were successfully analysed on the integrated slidable SPE-μPCR-μCE microdevice by using 1 μL whole human blood within 60 min. The proposed advanced genetic analysis microsystem is capable of point-of-care DNA testing with sample-in-answer-out capability, more importantly, without use of complicated microvalves and microtubing systems for liquid transfer. Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical Oxygen Demand abatement in sewage using Micro-Aeration Enhanced Ecological Floating Bed

NASA Astrophysics Data System (ADS)

Shi, Hongle; Zhou, Gaofeng; Liu, Yiqing; Tan, Jiancong; Fu, Yongsheng

2018-02-01

The traditional ecological floating bed combined with micro-aeration system and artificial medium was developed for the removal of contaminants and remediation of surface water. This micro-aeration enhanced ecological floating bed (MAEEFB) consisted of aeration unit, microbial processing unit and aquatic plant unit. Batch experiments were conducted in different operating conditions on the removal of chemical oxygen demand (COD) in the sewage using MAEEFB. The removal rate of COD by MAEEFB, enhanced ecological floating bed (EEFB) and traditional ecological floating bed (TEFB) in the same reaction conditions was 59.2%, 56.9% and 30.6%, respectively, indicating that the combination of micro-aeration system and artificial medium could enhance the removal efficiency of COD in TEFB. In MAEEFB, the aeration intensity should be designed reasonablely considering both treatment efficiency and operation cost. Only increasing the specific surface area of the packing cannot effectively improve the purification efficiency of water. Factors like packing material, ability of intercepting organics and complicated extent of microorganisms attaching on the packing should also be considered.

Experimental Methods to Characterize Nonlinear Vibration of Flapping Wing Micro Air Vehicles

DTIC Science & Technology

2007-03-01

EXPERIMENTAL METHODS TO CHARACTERIZE NONLINEAR VIBRATION OF FLAPPING WING MICRO AIR VEHICLES THESIS Adam P. Tobias, Captain, USAF...AFIT/GAE/ENY/07-M23 DEPARTMENT OF THE AIR FORCE AIR UNIVERSITY AIR FORCE INSTITUTE OF TECHNOLOGY Wright-Patterson Air Force Base, Ohio...author and do not reflect the official policy or position of the United States Air Force, Department of Defense, or the United States Government

The Effect of an Advanced Organizer and the Predictive Ability of Micro-Learning Tasks When Utilized with Carefully Sequenced Audio-Tutorial Units.

ERIC Educational Resources Information Center

Kahle, Jane Butler

Four audio-tutorial units were developed as part of this study to determine the effectiveness of the use of advanced organizers, based on Ausubel's theories, for meaningful learning experiences. In this study an advanced organizer was developed and given to half of the subjects prior to the instructional sequence. A series of micro-learning tasks,…

A novel piezoelectric quartz micro-array immunosensor for detection of immunoglobulinE.

PubMed

Yao, Chunyan; Chen, Qinghai; Chen, Ming; Zhang, Bo; Luo, Yang; Huang, Qing; Huang, Junfu; Fu, Weiling

2006-12-01

A novel multi-channel 2 x 5 model of piezoelectric (PZ) micro-array immunosensor has been developed for quantitative detection of human immunoglobulinE (IgE) in serum. Every crystal unit of the fabricated piezoelectric IgE micro-array immunosensor can oscillate without interfering each other. A multi-channel 2 x 5 model micro-array immunosensor as compared with the traditional one-channel immunosensor can provide eight times higher detection speeds for IgE assay. The anti-IgE antibody is deposited on the gold electrode's surface of 10 MHz AT-cut quartz crystals by SPA (staphylococcal protein A), and serves as an antibody recognizing layer. The highly ordered antibody monolayers ensure well-controlled surface structure and offer many advantages to the performance of the sensor. The uniform amount of antibody monolayer coated by the SPA is good, and non-specific reaction caused by other immunoglobulin in sample is found. The fabricated PZ immunosensor can be used for human IgE determination in the range of 5-300 IU/ml with high precision (CV is 4%). 50 human serum samples were detected by the micro-array immunosensor, and the results agreed well with those given by the commercially ELISA test kits. The correlation coefficient is 0.94 between ELISA and PZ immunosensor. After regeneration with NaOH the coated immunosensor can be reused 6 times without appreciable loss of activity.

Development of portable health monitoring system for automatic self-blood glucose measurement

NASA Astrophysics Data System (ADS)

Kim, Huijun; Mizuno, Yoshihumi; Nakamachi, Eiji; Morita, Yusuke

2010-02-01

In this study, a new HMS (Health Monitoring System) device is developed for diabetic patient. This device mainly consists of I) 3D blood vessel searching unit and II) automatic blood glucose measurement (ABGM) unit. This device has features such as 1)3D blood vessel location search 2) laptop type, 3) puncturing a blood vessel by using a minimally invasive micro-needle, 4) very little blood sampling (10μl), and 5) automatic blood extraction and blood glucose measurement. In this study, ABGM unit is described in detail. It employs a syringe type's blood extraction mechanism because of its high accuracy. And it consists of the syringe component and the driving component. The syringe component consists of a syringe itself, a piston, a magnet, a ratchet and a micro-needle whose inner diameter is about 80μm. And the syringe component is disposable. The driving component consists of body parts, a linear stepping motor, a glucose enzyme sensor and a slider for accurate positioning control. The driving component has the all-in-one mechanism with a glucose enzyme sensor for compact size and stable blood transfer. On designing, required thrust force to drive the slider is designed to be greater than the value of the blood extraction force. Further, only one linear stepping motor is employed for blood extraction and transportation processes. The experimental result showed more than 80% of volume ratio under the piston speed 2.4mm/s. Further, the blood glucose was measured successfully by using the prototype unit. Finally, the availability of our ABGM unit was confirmed.

Automatic-Control System for Safer Brazing

NASA Technical Reports Server (NTRS)

Stein, J. A.; Vanasse, M. A.

1986-01-01

Automatic-control system for radio-frequency (RF) induction brazing of metal tubing reduces probability of operator errors, increases safety, and ensures high-quality brazed joints. Unit combines functions of gas control and electric-power control. Minimizes unnecessary flow of argon gas into work area and prevents electrical shocks from RF terminals. Controller will not allow power to flow from RF generator to brazing head unless work has been firmly attached to head and has actuated micro-switch. Potential shock hazard eliminated. Flow of argon for purging and cooling must be turned on and adjusted before brazing power applied. Provision ensures power not applied prematurely, causing damaged work or poor-quality joints. Controller automatically turns off argon flow at conclusion of brazing so potentially suffocating gas does not accumulate in confined areas.

Airfoil-shaped micro-mixers for reducing fouling on membrane surfaces

DOEpatents

Ho, Clifford K; Altman, Susan J; Clem, Paul G; Hibbs, Michael; Cook, Adam W

2012-10-23

An array of airfoil-shaped micro-mixers that enhances fluid mixing within permeable membrane channels, such as used in reverse-osmosis filtration units, while minimizing additional pressure drop. The enhanced mixing reduces fouling of the membrane surfaces. The airfoil-shaped micro-mixer can also be coated with or comprised of biofouling-resistant (biocidal/germicidal) ingredients.

Graphics Processing Unit (GPU) implementation of image processing algorithms to improve system performance of the Control, Acquisition, Processing, and Image Display System (CAPIDS) of the Micro-Angiographic Fluoroscope (MAF).

PubMed

Vasan, S N Swetadri; Ionita, Ciprian N; Titus, A H; Cartwright, A N; Bednarek, D R; Rudin, S

2012-02-23

We present the image processing upgrades implemented on a Graphics Processing Unit (GPU) in the Control, Acquisition, Processing, and Image Display System (CAPIDS) for the custom Micro-Angiographic Fluoroscope (MAF) detector. Most of the image processing currently implemented in the CAPIDS system is pixel independent; that is, the operation on each pixel is the same and the operation on one does not depend upon the result from the operation on the other, allowing the entire image to be processed in parallel. GPU hardware was developed for this kind of massive parallel processing implementation. Thus for an algorithm which has a high amount of parallelism, a GPU implementation is much faster than a CPU implementation. The image processing algorithm upgrades implemented on the CAPIDS system include flat field correction, temporal filtering, image subtraction, roadmap mask generation and display window and leveling. A comparison between the previous and the upgraded version of CAPIDS has been presented, to demonstrate how the improvement is achieved. By performing the image processing on a GPU, significant improvements (with respect to timing or frame rate) have been achieved, including stable operation of the system at 30 fps during a fluoroscopy run, a DSA run, a roadmap procedure and automatic image windowing and leveling during each frame.

Optimal Control of Shock Wave Turbulent Boundary Layer Interactions Using Micro-Array Actuation

NASA Technical Reports Server (NTRS)

Anderson, Bernhard H.; Tinapple, Jon; Surber, Lewis

2006-01-01

The intent of this study on micro-array flow control is to demonstrate the viability and economy of Response Surface Methodology (RSM) to determine optimal designs of micro-array actuation for controlling the shock wave turbulent boundary layer interactions within supersonic inlets and compare these concepts to conventional bleed performance. The term micro-array refers to micro-actuator arrays which have heights of 25 to 40 percent of the undisturbed supersonic boundary layer thickness. This study covers optimal control of shock wave turbulent boundary layer interactions using standard micro-vane, tapered micro-vane, and standard micro-ramp arrays at a free stream Mach number of 2.0. The effectiveness of the three micro-array devices was tested using a shock pressure rise induced by the 10 shock generator, which was sufficiently strong as to separate the turbulent supersonic boundary layer. The overall design purpose of the micro-arrays was to alter the properties of the supersonic boundary layer by introducing a cascade of counter-rotating micro-vortices in the near wall region. In this manner, the impact of the shock wave boundary layer (SWBL) interaction on the main flow field was minimized without boundary bleed.

A portable hardware-in-the-loop (HIL) device for automotive diagnostic control systems.

PubMed

Palladino, A; Fiengo, G; Lanzo, D

2012-01-01

In-vehicle driving tests for evaluating the performance and diagnostic functionalities of engine control systems are often time consuming, expensive, and not reproducible. Using a hardware-in-the-loop (HIL) simulation approach, new control strategies and diagnostic functions on a controller area network (CAN) line can be easily tested in real time, in order to reduce the effort and the cost of the testing phase. Nowadays, spark ignition engines are controlled by an electronic control unit (ECU) with a large number of embedded sensors and actuators. In order to meet the rising demand of lower emissions and fuel consumption, an increasing number of control functions are added into such a unit. This work aims at presenting a portable electronic environment system, suited for HIL simulations, in order to test the engine control software and the diagnostic functionality on a CAN line, respectively, through non-regression and diagnostic tests. The performances of the proposed electronic device, called a micro hardware-in-the-loop system, are presented through the testing of the engine management system software of a 1.6 l Fiat gasoline engine with variable valve actuation for the ECU development version. Copyright © 2011 ISA. Published by Elsevier Ltd. All rights reserved.

Micro grid control strategy of DFIG unit based on improved DC grid connected topology

NASA Astrophysics Data System (ADS)

Zongze, Xia; Fei, Xia; Zhixiong, Yang

2017-05-01

Aiming to the application of the DFIG connected to DC-Microgrids, an improved topology for the DFIG connected to DC-Microgrids is taken into account in this thesis. The stator side loses the support of voltage and frequency of AC point of common coupling bus. A novel control method suitable to the stator side converter (SSC) and the rotor side converter (RSC) of the topology is proposed. The independent control of stator voltage and frequency, the decoupled control of power and variable speed constant frequency of DFIG are achieved in the doubly-fed induction generator connected to DC-Microgrids. which can enhance the capacity of active power transmission of DFIG during the voltage variation.

MicroRNA expression in benign breast tissue and risk of subsequent invasive breast cancer.

PubMed

Rohan, Thomas; Ye, Kenny; Wang, Yihong; Glass, Andrew G; Ginsberg, Mindy; Loudig, Olivier

2018-01-01

MicroRNAs are endogenous, small non-coding RNAs that control gene expression by directing their target mRNAs for degradation and/or posttranscriptional repression. Abnormal expression of microRNAs is thought to contribute to the development and progression of cancer. A history of benign breast disease (BBD) is associated with increased risk of subsequent breast cancer. However, no large-scale study has examined the association between microRNA expression in BBD tissue and risk of subsequent invasive breast cancer (IBC). We conducted discovery and validation case-control studies nested in a cohort of 15,395 women diagnosed with BBD in a large health plan between 1971 and 2006 and followed to mid-2015. Cases were women with BBD who developed subsequent IBC; controls were matched 1:1 to cases on age, age at diagnosis of BBD, and duration of plan membership. The discovery stage (316 case-control pairs) entailed use of the Illumina MicroRNA Expression Profiling Assay (in duplicate) to identify breast cancer-associated microRNAs. MicroRNAs identified at this stage were ranked by the strength of the correlation between Illumina array and quantitative PCR results for 15 case-control pairs. The top ranked 14 microRNAs entered the validation stage (165 case-control pairs) which was conducted using quantitative PCR (in triplicate). In both stages, linear regression was used to evaluate the association between the mean expression level of each microRNA (response variable) and case-control status (independent variable); paired t-tests were also used in the validation stage. None of the 14 validation stage microRNAs was associated with breast cancer risk. The results of this study suggest that microRNA expression in benign breast tissue does not influence the risk of subsequent IBC.

MicroRNA expression in benign breast tissue and risk of subsequent invasive breast cancer

PubMed Central

Ye, Kenny; Wang, Yihong; Ginsberg, Mindy; Loudig, Olivier

2018-01-01

MicroRNAs are endogenous, small non-coding RNAs that control gene expression by directing their target mRNAs for degradation and/or posttranscriptional repression. Abnormal expression of microRNAs is thought to contribute to the development and progression of cancer. A history of benign breast disease (BBD) is associated with increased risk of subsequent breast cancer. However, no large-scale study has examined the association between microRNA expression in BBD tissue and risk of subsequent invasive breast cancer (IBC). We conducted discovery and validation case-control studies nested in a cohort of 15,395 women diagnosed with BBD in a large health plan between 1971 and 2006 and followed to mid-2015. Cases were women with BBD who developed subsequent IBC; controls were matched 1:1 to cases on age, age at diagnosis of BBD, and duration of plan membership. The discovery stage (316 case-control pairs) entailed use of the Illumina MicroRNA Expression Profiling Assay (in duplicate) to identify breast cancer-associated microRNAs. MicroRNAs identified at this stage were ranked by the strength of the correlation between Illumina array and quantitative PCR results for 15 case-control pairs. The top ranked 14 microRNAs entered the validation stage (165 case-control pairs) which was conducted using quantitative PCR (in triplicate). In both stages, linear regression was used to evaluate the association between the mean expression level of each microRNA (response variable) and case-control status (independent variable); paired t-tests were also used in the validation stage. None of the 14 validation stage microRNAs was associated with breast cancer risk. The results of this study suggest that microRNA expression in benign breast tissue does not influence the risk of subsequent IBC. PMID:29432432

Online Optimization Method for Operation of Generators in a Micro Grid

NASA Astrophysics Data System (ADS)

Hayashi, Yasuhiro; Miyamoto, Hideki; Matsuki, Junya; Iizuka, Toshio; Azuma, Hitoshi

Recently a lot of studies and developments about distributed generator such as photovoltaic generation system, wind turbine generation system and fuel cell have been performed under the background of the global environment issues and deregulation of the electricity market, and the technique of these distributed generators have progressed. Especially, micro grid which consists of several distributed generators, loads and storage battery is expected as one of the new operation system of distributed generator. However, since precipitous load fluctuation occurs in micro grid for the reason of its smaller capacity compared with conventional power system, high-accuracy load forecasting and control scheme to balance of supply and demand are needed. Namely, it is necessary to improve the precision of operation in micro grid by observing load fluctuation and correcting start-stop schedule and output of generators online. But it is not easy to determine the operation schedule of each generator in short time, because the problem to determine start-up, shut-down and output of each generator in micro grid is a mixed integer programming problem. In this paper, the authors propose an online optimization method for the optimal operation schedule of generators in micro grid. The proposed method is based on enumeration method and particle swarm optimization (PSO). In the proposed method, after picking up all unit commitment patterns of each generators satisfied with minimum up time and minimum down time constraint by using enumeration method, optimal schedule and output of generators are determined under the other operational constraints by using PSO. Numerical simulation is carried out for a micro grid model with five generators and photovoltaic generation system in order to examine the validity of the proposed method.

Intermittent micro-aeration control of methane emissions from an integrated vertical-flow constructed wetland during agricultural domestic wastewater treatment.

PubMed

Liu, Xiaoling; Zhang, Ke; Fan, Liangqian; Luo, Hongbing; Jiang, Mingshu; Anderson, Bruce C; Li, Mei; Huang, Bo; Yu, Lijuan; He, Guozhu; Wang, Jingting; Pu, Aiping

2018-06-16

It is very important to control methane emissions to mitigate global warming. An intermittent micro-aeration control system was used to control methane emissions from an integrated vertical-flow constructed wetland (IVCW) to treat agricultural domestic wastewater pollution in this study. The optimized intermittent micro-aeration conditions were a 20-min aeration time and 340-min non-aeration time, 3.9 m 3  h -1 aeration intensity, evenly distributed micro-aeration diffusers at the tank bottom, and an aeration period of every 6 h. Methane flux emission by intermittent micro-aeration was decreased by 60.7% under the optimized conditions. The average oxygen transfer efficiency was 26.73%. The control of CH 4 emission from IVCWs was most strongly influenced by the intermittent micro-aeration diffuser distribution, followed by aeration intensity, aeration time, and water depth. Scaling up of IVCWs is feasible in rural areas by using intermittent micro-aeration control as a mitigation measure for methane gas emissions for climate change.

Efficient system interrupt concept design at the microprogramming level

DOE Office of Scientific and Technical Information (OSTI.GOV)

Fakharzadeh, M.M.

1989-01-01

Over the past decade the demand for high speed super microcomputers has been tremendously increased. To satisfy this demand many high speed 32-bit microcomputers have been designed. However, the currently available 32-bit systems do not provide an adequate solution to many highly demanding problems such as in multitasking, and in interrupt driven applications, which both require context switching. Systems for these purposes usually incorporate sophisticated software. In order to be efficient, a high end microprocessor based system must satisfy stringent software demands. Although these microprocessors use the latest technology in the fabrication design and run at a very high speed,more » they still suffer from insufficient hardware support for such applications. All too often, this lack also is the premier cause of execution inefficiency. In this dissertation a micro-programmable control unit and operation unit is considered in an advanced design. An automaton controller is designed for high speed micro-level interrupt handling. Different stack models are designed for the single task and multitasking environment. The stacks are used for storage of various components of the processor during the interrupt calls, procedure calls, and task switching. A universal (as an example seven port) register file is designed for high speed parameter passing, and intertask communication in the multitasking environment. In addition, the register file provides a direct path between ALU and the peripheral data which is important in real-time control applications. The overall system is a highly parallel architecture, with no pipeline and internal cache memory, which allows the designer to be able to predict the processor's behavior during the critical times.« less

Andy Jenkins Builds Applications Development For Lab-on-a-Chip

NASA Technical Reports Server (NTRS)

2004-01-01

Andy Jenkins, an engineer for the Lab on a Chip Applications Development program, helped build the Applications Development Unit (ADU-25), a one-of-a-kind facility for controlling and analyzing processes on chips with extreme accuracy. Pressure is used to cause fluids to travel through network of fluid pathways, or micro-channels, embossed on the chips through a process similar to the one used to print circuits on computer chips. To make customized chips for various applications, NASA has an agreement with the U.S. Army's Micro devices and Micro fabrication Laboratory at Redstone Arsenal in Huntsville, Alabama, where NASA's Marshall Space Flight Center (MSFC) is located. The Marshall Center team is also collaborating with scientists at other NASA centers and at universities to develop custom chip designs for many applications, such as studying how fluidic systems work in spacecraft and identifying microbes in self-contained life support systems. Chips could even be designed for use on Earth, such as for detecting deadly microbes in heating and air systems. (NASA/MSFC/D.Stoffer)

Sampling strategies exploiting multi-pumping flow systems.

PubMed

Prior, João A V; Santos, João L M; Lima, José L F C

2003-04-01

In this work new strategies were exploited to implement multi-pumping flow systems relying on the utilisation of multiple devices that act simultaneously as sample-insertion, reagent-introduction, and solution-propelling units. The solenoid micro-pumps that were initially used as the only active elements of multi-pumping systems, and which were able to produce pulses of 3 to 25 microL, were replaced by syringe pumps with the aim of producing pulses between 1 and 4 microL. The performance of the developed flow system was assessed by using distinct sample-insertion strategies like single sample volume, merging zones, and binary sampling in the spectrophotometric determination of isoniazid in pharmaceutical formulations upon reaction with 1,2-naphthoquinone-4-sulfonate, in alkaline medium. The results obtained showed that enhanced sample/reagent mixing could be obtained with binary sampling and by using a 1 microL per step pump, even in limited dispersion conditions. Moreover, syringe pumps produce very reproducible flowing streams and are easily manipulated and controlled by a computer program, which is greatly simplified since they are the only active manifold component. Linear calibration plots up to 18.0 microg mL(-1), with a relative standard deviation of less than 1.48% (n=10) and a throughput of about 20 samples per hour, were obtained.

Theoretical and experimental analysis of AlGaInP micro-LED array with square-circle anode

NASA Astrophysics Data System (ADS)

Tian, Chao; Wang, Weibiao; Liang, Jingqiu; Liang, Zhongzhu; Qin, Yuxin; Lv, Jinguang

2015-04-01

An array of 320 × 240 micro-light-emitting diodes (micro-LEDs) based on an AlGaInP epitaxial wafer and with a unit size of 100 µm×100 µm was designed and fabricated. The optimum width of the isolation groove between adjacent light-emitting units was determined based on a compromise between full isolation of each LED and maximization of the light emitting area, and was found to be 20 µm. The grooves were filled with a mixed Si granule-polyurethane composite medium, because this type of insulating material can reflect part of the emitted light from the sidewall to the window layer in each light-emitting unit, and could thus improve lighting output efficiency. The 10-µm-wide square-circle anode was designed to increase the light emitting area while simultaneously being simple to fabricate. The device current used was in the 0.42-1.06 mA range to guarantee internal quantum efficiency of more than 85%, with a corresponding voltage range of 2-2.3 V. The layered temperature distribution in a single unit was simulated under a drive voltage of 2.2 V, and the maximum device temperature was 341 K. The micro-opto-electro-mechanical systems (MOEMS) technology-based fabrication process, experimental images of the device and device test results are presented here.

Theoretical and experimental analysis of AlGaInP micro-LED array with square-circle anode

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tian, Chao; University of Chinese Academy of Sciences, Beijing 100049; Wang, Weibiao, E-mail: wangwbcn@163.com

An array of 320 × 240 micro-light-emitting diodes (micro-LEDs) based on an AlGaInP epitaxial wafer and with a unit size of 100 µm×100 µm was designed and fabricated. The optimum width of the isolation groove between adjacent light-emitting units was determined based on a compromise between full isolation of each LED and maximization of the light emitting area, and was found to be 20 µm. The grooves were filled with a mixed Si granule-polyurethane composite medium, because this type of insulating material can reflect part of the emitted light from the sidewall to the window layer in each light-emitting unit,more » and could thus improve lighting output efficiency. The 10-µm-wide square-circle anode was designed to increase the light emitting area while simultaneously being simple to fabricate. The device current used was in the 0.42–1.06 mA range to guarantee internal quantum efficiency of more than 85%, with a corresponding voltage range of 2–2.3 V. The layered temperature distribution in a single unit was simulated under a drive voltage of 2.2 V, and the maximum device temperature was 341 K. The micro-opto-electro-mechanical systems (MOEMS) technology-based fabrication process, experimental images of the device and device test results are presented here.« less

Micro-cogen AMTEC systems for residential and transportation opportunities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Mital, R.; Rasmussen, J.R.; Hunt, T.

1998-07-01

This paper describes the design and anticipated performance of high efficiency AMTEC systems suitable for natural gas fired micro-cogeneration for residential and transportation applications. AMTEC systems have a relatively flat efficiency curve from a few tens of watts to several kilowatts. This unique quality of AMTEC makes it well suited for micro-cogen as opposed to other technologies, such as internal combustion (IC) engines, which lose efficiency at low power levels. AMTEC also offers additional advantages of high efficiency, high reliability, low noise and low emissions. Combustion heated AMTEC cogeneration systems can also be used in trucks and trailers to keepmore » the diesel engines and cabs warm, provide electrical power for charging the battery and maintain power to the electrical systems during stand down periods. A market study indicates that residential micro-cogen units should have a design generating capacity between 0.5--2 kW. AMTEC systems producing 500 W net electric power have been designed and are presently being built. A 350 W prototype unit is being manufactured for a European firm as a trial unit for central heat and power from a home furnace. Modular one kilowatt units are also being designed that will allow combination into multi-kilowatt systems. The results of feasibility studies focused on price/Watt, efficiency, noise, emission, vibrations, expected lifetime and maintenance cost are also presented in this paper.« less

Life cycle of Arabidopsis thaliana under microgravity condition in the International Space Station Kibo module

NASA Astrophysics Data System (ADS)

Karahara, Ichirou; Soga, Kouichi; Hoson, Takayuki; Kamisaka, Seiichiro; Yano, Sachiko; Shimazu, Toru; Tamaoki, Daisuke; Tanigaki, Fumiaki; Kasahara, Haruo; Yashiro, Umi; Suto, Takamichi; Yamaguchi, Takashi; Kasahara, Hirokazu

2012-07-01

Gravity is an important environmental factors for growth and development of plants throughout their life cycle. We have designed an experiment, which is called Space Seed, to examine the effects of microgravity on the seed to seed life cycle of plants. We have carried out this experiment using a newly developed apparatus, which is called the Plant Experiment Unit (PEU) and installed in the Cell Biology Experiment Facility (CBEF) onboard International Space Station (ISS). The CBEF is equipped with a turntable generating artificial gravity to perform 1-G control experiment as well as micro-G experiment on board. Arabidopsis thaliana seeds sown on dry rockwool in PEUs were transported from Kennedy Space Center to the ISS Kibo module by Space Shuttle Discovery in STS-128 mission. This experiment was started on Sep. 10, 2009 and terminated on Nov. 11, 2009. Arabidopsis seeds successfully germinated, and the plants passed through both vegetative and reproductive processes, such as formation of rosette leaves, bolting of inflorescence stems, flowering, formation of siliques and seeds. Vegetative and reproductive growth were compared among micro-G plants, 1-G control, and the ground control.

Controlling Variable Emittance (MEMS) Coatings for Space Applications

NASA Technical Reports Server (NTRS)

Farrar, D.; Schneider, W.; Osiander, R.; Champion, J. L.; Darrin, A. G.; Douglas, Donya; Swanson, Ted D.

2003-01-01

Small spacecraft, including micro and nanosats, as they are envisioned for future missions, will require an alternative means to achieve thermal control due to their small power and mass budgets. One of the proposed alternatives is Variable Emittance (Vari-E) Coatings for spacecraft radiators. Space Technology-5 (ST-5) is a technology demonstration mission through NASA Goddard Space Flight Center (GSFC) that will utilize Vari-E Coatings. This mission involves a constellation of three (3) satellites in a highly elliptical orbit with a perigee altitude of approximately 200 kilometers and an apogee of approximately 38,000 kilometers. Such an environment will expose the spacecraft to a wide swing in the thermal and radiation environment of the earth's atmosphere. There are three (3) different technologies associated with this mission. The three technologies are electrophoretic, electrochromic, and Micro ElectroMechanical Systems (MEMS). The ultimate goal is to make use of Van-E coatings, in order to achieve various levels of thermal control. The focus of this paper is to highlight the Vari-E Coating MEMS instrument, with an emphasis on the Electronic Control Unit responsible for operating the MEMS device. The Test & Evaluation approach, along with the results, is specific for application on ST-5, yet the information provides a guideline for future experiments and/or thermal applications on the exterior structure of a spacecraft.

Data set on the effects of conifer control and slash burning on soil carbon, total N, organic matter and extractable micro-nutrients.

PubMed

Bates, Jonathan D; Davies, Kirk W

2017-10-01

Conifer control in sagebrush steppe of the western United States causes various levels of site disturbance influencing vegetation recovery and resource availability. The data set presented in this article include growing season availability of soil micronutrients and levels of total soil carbon, organic matter, and N spanning a six year period following western juniper ( Juniperus occidentalis spp. occidentalis ) reduction by mechanical cutting and prescribed fire of western juniper woodlands in southeast Oregon. These data can be useful to further evaluate the impacts of conifer woodland reduction to soil resources in sagebrush steppe plant communities.

Feedforward hysteresis compensation in trajectory control of piezoelectrically-driven nanostagers

NASA Astrophysics Data System (ADS)

Bashash, Saeid; Jalili, Nader

2006-03-01

Complex structural nonlinearities of piezoelectric materials drastically degrade their performance in variety of micro- and nano-positioning applications. From the precision positioning and control perspective, the multi-path time-history dependent hysteresis phenomenon is the most concerned nonlinearity in piezoelectric actuators to be analyzed. To realize the underlying physics of this phenomenon and to develop an efficient compensation strategy, the intelligent properties of hysteresis with the effects of non-local memories are discussed. Through performing a set of experiments on a piezoelectrically-driven nanostager with high resolution capacitive position sensor, it is shown that for the precise prediction of hysteresis path, certain memory units are required to store the previous hysteresis trajectory data. Based on the experimental observations, a constitutive memory-based mathematical modeling framework is developed and trained for the precise prediction of hysteresis path for arbitrarily assigned input profiles. Using the inverse hysteresis model, a feedforward control strategy is then developed and implemented on the nanostager to compensate for the system everpresent nonlinearity. Experimental results demonstrate that the controller remarkably eliminates the nonlinear effect if memory units are sufficiently chosen for the inverse model.

Firing rate modulation of human motor units in different muscles during isometric contraction with various forces.

PubMed

Seki, K; Narusawa, M

1996-05-06

To examine the factors affecting the control of human motor units, rate coding strategies of the motor units were investigated in upper limb and intrinsic hand muscles during voluntary isometric contraction of steady force levels up to 80% of maximal voluntary contraction. Numerous spike trains from single motor units were recorded from the m. first dorsal interosseous (FDI) and the m. biceps brachii (BB) of eight human subjects by means of tungsten micro-electrodes, and the mean firing rate (MFR) was calculated for each subject and inter-individual comparisons made. The MFRs of the FDI were larger than that of the BB at the higher force level, and substantial differences were not found between these muscles at the lower force level. The slope of the linear regression line of MFRs vs. exerted forces for the FDI was more than twice that for the BB. Therefore, isometric force control of the FDI depends more on the rate coding strategy. The difference in rate coding between the FDI and BB motor units may be determined by factors other than muscle fiber composition, because both muscles are known to possess a similar composition of fiber types. Possible mechanisms underlying these characteristics of rate coding strategy are considered in this report.

Design and implementation of an omni-directional underwater acoustic micro-modem based on a low-power micro-controller unit.

PubMed

Won, Tae-Hee; Park, Sung-Joon

2012-01-01

For decades, underwater acoustic communication has been restricted to the point-to-point long distance applications such as deep sea probes and offshore oil fields. For this reason, previous acoustic modems were typically characterized by high data rates and long working ranges at the expense of large size and high power consumption. Recently, as the need for underwater wireless sensor networks (UWSNs) has increased, the research and development of compact and low-power consuming communication devices has become the focus. From the consideration that the requisites of acoustic modems for UWSNs are low power consumption, omni-directional beam pattern, low cost and so on, in this paper, we design and implement an omni-directional underwater acoustic micro-modem satisfying these requirements. In order to execute fast digital domain signal processing and support flexible interfaces with other peripherals, an ARM Cortex-M3 is embedded in the micro-modem. Also, for the realization of small and omni-directional properties, a spherical transducer having a resonant frequency of 70 kHz and a diameter of 34 mm is utilized for the implementation. Physical layer frame format and symbol structure for efficient packet-based underwater communication systems are also investigated. The developed acoustic micro-modem is verified analytically and experimentally in indoor and outdoor environments in terms of functionality and performance. Since the modem satisfies the requirements for use in UWSNs, it could be deployed in a wide range of applications requiring underwater acoustic communication.

Delivery of Colloid Micro-Newton Thrusters for the Space Technology 7 Mission

NASA Technical Reports Server (NTRS)

Ziemer, John K.; Randolph, Thomas M.; Franklin, Garth W.; Hruby, Vlad; Spence, Douglas; Demmons, Nathaniel; Roy, Thomas; Ehrbar, Eric; Zwahlen, Jurg; Martin, Roy;

Effects of methyltestosterone on insulin secretion and sensitivity in women.

PubMed

Diamond, M P; Grainger, D; Diamond, M C; Sherwin, R S; Defronzo, R A

1998-12-01

The frequent coexistence of hyperandrogenism and insulin resistance is well established; however, whether a cause and effect relationship exists remains to be established. In this study we tested the hypothesis that short-term androgen administered to women would induce insulin resistance. To test this hypothesis, regularly menstruating, nonobese women were studied before and during methyltestosterone administration (5 mg tid for 10-12 days) by the hyperglycemic (n=8) and euglycemic, hyperinsulinemic (n=7) clamp techniques. Short-term methyltestosterone administration had no significant effects on the fasting levels of glucose, insulin, c-peptide, glucagon, or glucose turnover. During the hyperglycemic clamp studies, the mean glucose level during the final hour was 203+/-2 and 201+/-1 mg/dL in the methyltestosterone and control studies, respectively. The insulin response to this hyperglycemic challenge was slightly but not significantly greater during methyltestosterone treatment (first phase 59+/-8 vs. 50+/-8 microU/mL in controls; second phase 74+/-9 vs. 67+/-9 microU/mL in controls; total insulin response 133+/-16 vs. 117+/-15 microU/mL in controls). In spite of this, glucose uptake was reduced from the control study value of 10.96+/-1.11 to 7.3+/-0.70 mg/kg/min by methyltestosterone (P < 0.05). The ratio of glucose uptake per unit of insulin was also significantly reduced from a control study value of 14.3+/-1.4 to 9.4+/-1.3 mg/kg/min per microU/mL x 100 during methyltestosterone administration. In the euglycemic hyperinsulinemic clamp studies, insulin was infused at rates of 0.25 and 1.0 mU/kg/min to achieve insulin levels of approximately 25 and 68 microU/mL, respectively. During low-dose insulin infusion, rates of endogenous hepatic glucose production were equivalently suppressed from basal values of 2.37+/-0.29 and 2.40+/-0.27 mg/kg/min to 0.88+/-0.25 and 0.77+/-0.26 mg/kg/min in the methyltestesterone and control studies respectively. Whole body glucose uptake during low-dose insulin infusion was minimally affected. During the high-dose insulin infusion, endogenous hepatic glucose production was nearly totally suppressed in both groups. However, whole body glucose uptake was reduced from the control value of 6.11+/-0.49 mg/kg/min to 4.93+/-0.44 mg/kg/min during methyltestosterone administration (P < 0.05). Our data demonstrate that androgen excess leads to the development of insulin resistance during both hyperglycemic and euglycemic hyperinsulinemia. These findings provide direct evidence for a relationship between hyperandrogenemia and insulin resistance, and its associated risk factors for cardiovascular disease.

Microfabrication and Test of a Three-Dimensional Polymer Hydro-focusing Unit for Flow Cytometry Applications

NASA Technical Reports Server (NTRS)

Yang, Ren; Feeback, Daniel L.; Wang, Wanjun

2004-01-01

This paper details a novel three-dimensional (3D) hydro-focusing micro cell sorter for micro flow cytometry applications. The unit was microfabricated by means of SU-8 3D lithography. The 3D microstructure for coaxial sheathing was designed, microfabricated, and tested. Three-dimensional hydro-focusing capability was demonstrated with an experiment to sort labeled tanned sheep erythrocytes (red blood cells). This polymer hydro-focusing microstructure is easily microfabricated and integrated with other polymer microfluidic structures.

Calcium Chloride in Neonatal Parenteral Nutrition Solutions with and without Added Cysteine: Compatibility Studies Using Laser and Micro-Flow Imaging Methodology.

PubMed

Huston, Robert K; Christensen, J Mark; Alshahrani, Sultan M; Mohamed, Sumeia M; Clark, Sara M; Nason, Jeffrey A; Wu, Ying Xing

2015-01-01

Previous studies of compatibility of calcium chloride (CaCl2) and phosphates have not included particle counts in the range specified by the United States Pharmacopeia. Micro-flow imaging techniques have been shown to be comparable to light obscuration when determining particle count and size in pharmaceutical solutions. The purpose of this study was to do compatibility testing for parenteral nutrition (PN) solutions containing CaCl2 using dynamic light scattering and micro-flow imaging techniques. Solutions containing TrophAmine (Braun Medical Inc, Irvine, CA), CaCl2, and sodium phosphate (NaPhos) were compounded with and without cysteine. All solutions contained standard additives to neonatal PN solutions including dextrose, trace metals, and electrolytes. Control solutions contained no calcium or phosphate. Solutions were analyzed for particle size and particle count. Means of Z-average particle size and particle counts of controls were determined. Study solutions were compared to controls and United States Pharmacopeia (USP) Chapter 788 guidelines. The maximum amount of Phos that was compatible in solutions that contained at least 10 mmol/L of Ca in 2.5% amino acids (AA) was determined. Compatibility of these solutions was verified by performing analyses of 5 repeats of these solutions. Microscopic analyses of the repeats were also performed. Amounts of CaCl2 and NaPhos that were compatible in solutions containing 1.5%, 2%, 2.5%, and 3% AA were determined. The maximum amount of NaPhos that could be added to TrophAmine solutions of > = 2.5% AA containing at least 10 mmol/L of CaCl2 was 7.5 mmol/L. Adding 50 mg/dL of cysteine increased the amount of NaPhos that could be added to solutions containing 10 mmol/L of CaCl2 to 10 mmol/L. Calcium chloride can be added to neonatal PN solutions containing NaPhos in concentrations that can potentially provide an intravenous intake of adequate amounts of calcium and phosphorus.

Low-power grating detection system chip for high-speed low-cost length and angle precision measurement

NASA Astrophysics Data System (ADS)

Hou, Ligang; Luo, Rengui; Wu, Wuchen

2006-11-01

This paper forwards a low power grating detection chip (EYAS) on length and angle precision measurement. Traditional grating detection method, such as resister chain divide or phase locked divide circuit are difficult to design and tune. The need of an additional CPU for control and display makes these methods' implementation more complex and costly. Traditional methods also suffer low sampling speed for the complex divide circuit scheme and CPU software compensation. EYAS is an application specific integrated circuit (ASIC). It integrates micro controller unit (MCU), power management unit (PMU), LCD controller, Keyboard interface, grating detection unit and other peripherals. Working at 10MHz, EYAS can afford 5MHz internal sampling rate and can handle 1.25MHz orthogonal signal from grating sensor. With a simple control interface by keyboard, sensor parameter, data processing and system working mode can be configured. Two LCD controllers can adapt to dot array LCD or segment bit LCD, which comprised output interface. PMU alters system between working and standby mode by clock gating technique to save power. EYAS in test mode (system action are more frequently than real world use) consumes 0.9mw, while 0.2mw in real world use. EYAS achieved the whole grating detection system function, high-speed orthogonal signal handling in a single chip with very low power consumption.

Sensitivity enhancement in optical micro-tube resonator sensors via mode coupling

NASA Astrophysics Data System (ADS)

Ling, Tao; Guo, L. Jay

2013-07-01

A liquid filled, silica micro-tube with a low refractive index material inner-coating has been proposed and theoretically studied as a coupled micro-resonator sensor to greatly enhance biochemical sensor sensitivity. Its unique coupling phenomenon has been analyzed and utilized to boost the device's refractive index sensitivity to 967 nm/Refractive Index Unit (RIU). Through optimization of the coupling strength between the two micro-resonators, further improvement in refractive index sensitivity up to 1100 nm/RIU has been predicted. This mode coupling strategy allows us to design robust, thick-walled micro-tube sensors with ultra-high sensitivity which is useful in practical biochemical sensing applications.

Effect of healing time on bone-implant contact of orthodontic micro-implants: a histologic study.

PubMed

Ramazanzadeh, Barat Ali; Fatemi, Kazem; Dehghani, Mahboobe; Mohtasham, Nooshin; Jahanbin, Arezoo; Sadeghian, Hamed

2014-01-01

Objectives. This study aimed to evaluate the effect of immediate and delayed loading of orthodontic micro-implants on bone-implant contact. Materials and Methods. Sixty four micro-implants were implanted in dog's jaw bone. The micro-implants were divided into loaded and unloaded (control) groups. The control group had two subgroups: four and eight weeks being implanted. The loaded group had two subgroups of immediate loading and delayed (after four weeks healing) loading. Loaded samples were subjected to 200g load for four weeks. After sacrificing the animals micro-implants and surrounding tissues were observed histologically. Bone-implant contact ratios (BIC) were calculated and different groups' results were compared by three-way ANOVA. Results. Mean survival rate was 96.7% in general. Survival rates were 96.7%, 94.4% and 100% for control, immediate and delayed loaded groups, respectively. BIC values were not significantly different in loaded and control groups, immediate and delayed loading groups, and pressure and tension sides. Mandibular micro-implants had significantly higher BIC than maxillary ones in immediate loading, 4-weeks control, and 8-weeks control groups (P = 0.021, P = 0.009, P = 0.003, resp.). Conclusion Immediate or delayed loading of micro-implants in dog did not cause significant difference in Bone-implant contact which could be concluded that healing time had not significant effect on micro-implant stability.

Effect of Healing Time on Bone-Implant Contact of Orthodontic Micro-Implants: A Histologic Study

PubMed Central

Ramazanzadeh, Barat Ali; Fatemi, Kazem; Dehghani, Mahboobe; Mohtasham, Nooshin; Jahanbin, Arezoo; Sadeghian, Hamed

2014-01-01

Objectives. This study aimed to evaluate the effect of immediate and delayed loading of orthodontic micro-implants on bone-implant contact. Materials and Methods. Sixty four micro-implants were implanted in dog's jaw bone. The micro-implants were divided into loaded and unloaded (control) groups. The control group had two subgroups: four and eight weeks being implanted. The loaded group had two subgroups of immediate loading and delayed (after four weeks healing) loading. Loaded samples were subjected to 200g load for four weeks. After sacrificing the animals micro-implants and surrounding tissues were observed histologically. Bone-implant contact ratios (BIC) were calculated and different groups' results were compared by three-way ANOVA. Results. Mean survival rate was 96.7% in general. Survival rates were 96.7%, 94.4% and 100% for control, immediate and delayed loaded groups, respectively. BIC values were not significantly different in loaded and control groups, immediate and delayed loading groups, and pressure and tension sides. Mandibular micro-implants had significantly higher BIC than maxillary ones in immediate loading, 4-weeks control, and 8-weeks control groups (P = 0.021, P = 0.009, P = 0.003, resp.). Conclusion Immediate or delayed loading of micro-implants in dog did not cause significant difference in Bone-implant contact which could be concluded that healing time had not significant effect on micro-implant stability. PMID:25006463

Fabrication of micro-lens array on convex surface by meaning of micro-milling

NASA Astrophysics Data System (ADS)

Zhang, Peng; Du, Yunlong; Wang, Bo; Shan, Debin

2014-08-01

In order to develop the application of the micro-milling technology, and to fabricate ultra-precision optical surface with complex microstructure, in this paper, the primary experimental research on micro-milling complex microstructure array is carried out. A complex microstructure array surface with vary parameters is designed, and the mathematic model of the surface is set up and simulated. For the fabrication of the designed microstructure array surface, a micro three-axis ultra-precision milling machine tool is developed, aerostatic guideway drove directly by linear motor is adopted in order to guarantee the enough stiffness of the machine, and novel numerical control strategy with linear encoders of 5nm resolution used as the feedback of the control system is employed to ensure the extremely high motion control accuracy. With the help of CAD/CAM technology, convex micro lens array on convex spherical surface with different scales on material of polyvinyl chloride (PVC) and pure copper is fabricated using micro tungsten carbide ball end milling tool based on the ultra-precision micro-milling machine. Excellent nanometer-level micro-movement performance of the axis is proved by motion control experiment. The fabrication is nearly as the same as the design, the characteristic scale of the microstructure is less than 200μm and the accuracy is better than 1μm. It prove that ultra-precision micro-milling technology based on micro ultra-precision machine tool is a suitable and optional method for micro manufacture of microstructure array surface on different kinds of materials, and with the development of micro milling cutter, ultraprecision micro-milling complex microstructure surface will be achieved in future.

The study on the control strategy of micro grid considering the economy of energy storage operation

NASA Astrophysics Data System (ADS)

Ma, Zhiwei; Liu, Yiqun; Wang, Xin; Li, Bei; Zeng, Ming

2017-08-01

To optimize the running of micro grid to guarantee the supply and demand balance of electricity, and to promote the utilization of renewable energy. The control strategy of micro grid energy storage system is studied. Firstly, the mixed integer linear programming model is established based on the receding horizon control. Secondly, the modified cuckoo search algorithm is proposed to calculate the model. Finally, a case study is carried out to study the signal characteristic of micro grid and batteries under the optimal control strategy, and the convergence of the modified cuckoo search algorithm is compared with others to verify the validity of the proposed model and method. The results show that, different micro grid running targets can affect the control strategy of energy storage system, which further affect the signal characteristics of the micro grid. Meanwhile, the convergent speed, computing time and the economy of the modified cuckoo search algorithm are improved compared with the traditional cuckoo search algorithm and differential evolution algorithm.

Optimal Micro-Jet Flow Control for Compact Air Vehicle Inlets

NASA Technical Reports Server (NTRS)

Anderson, Bernhard H.; Miller, Daniel N.; Addington, Gregory A.; Agrell, Johan

2004-01-01

The purpose of this study on micro-jet secondary flow control is to demonstrate the viability and economy of Response Surface Methodology (RSM) to optimally design micro-jet secondary flow control arrays, and to establish that the aeromechanical effects of engine face distortion can also be included in the design and optimization process. These statistical design concepts were used to investigate the design characteristics of "low mass" micro-jet array designs. The term "low mass" micro-jet may refers to fluidic jets with total (integrated) mass flow ratios between 0.10 and 1.0 percent of the engine face mass flow. Therefore, this report examines optimal micro-jet array designs for compact inlets through a Response Surface Methodology.

Study on a magnetic spiral-type wireless capsule endoscope controlled by rotational external permanent magnet

NASA Astrophysics Data System (ADS)

Ye, Bo; Zhang, Wei; Sun, Zhen-jun; Guo, Lin; Deng, Chao; Chen, Ya-qi; Zhang, Hong-hai; Liu, Sheng

2015-12-01

In this paper, the authors propose rotating an external permanent magnet (EPM) to manipulate the synchronous rotation of a magnetic spiral-type wireless capsule endoscope (WCE), and the synchronous rotation of the WCE is converted to its translational motion in intestinal tract. In order to preliminarily verify the feasibility of this method, a handheld actuator (HA) controlled by micro controller unit, a magnetic spiral-type WCE and a bracket were fabricated, theoretical analysis and simulations about the control distance of this method were performed, and in ex-vivo tests were examined in porcine small intestine to verify the control distance and control performances of this method. It was demonstrated that this method showed good performances in controlling the translational motion of the magnetic spiral-type WCE, and this method has great potential to be used in clinical application.

Intelligent Control of Micro Grid: A Big Data-Based Control Center

NASA Astrophysics Data System (ADS)

Liu, Lu; Wang, Yanping; Liu, Li; Wang, Zhiseng

2018-01-01

In this paper, a structure of micro grid system with big data-based control center is introduced. Energy data from distributed generation, storage and load are analized through the control center, and from the results new trends will be predicted and applied as a feedback to optimize the control. Therefore, each step proceeded in micro grid can be adjusted and orgnized in a form of comprehensive management. A framework of real-time data collection, data processing and data analysis will be proposed by employing big data technology. Consequently, a integrated distributed generation and a optimized energy storage and transmission process can be implemented in the micro grid system.

Tracking Control and System Development for Laser-Driven Micro-Vehicles

NASA Astrophysics Data System (ADS)

Kajiwara, Itsuro; Hoshino, Kentaro; Hara, Shinji; Shiokata, Daisuke; Yabe, Takashi

The purpose of this paper is to design a control system for an integrated laser propulsion/tracking system to achieve continuous motion and control of laser-driven micro-vehicles. Laser propulsion is significant in achieving miniature and light micro-vehicles. A laser-driven micro-airplane has been studied using a paper airplane and YAG laser, resulting in successful gliding of the airplane. High-performance laser tracking control is required to achieve continuous flight. This paper presents a control design strategy based on the generalized Kalman-Yakubovic-Popov lemma to achieve this requirement. Experiments have been carried out to evaluate the performance of the integrated laser propulsion/tracking system.

Reinsurance of health insurance for the informal sector.

PubMed Central

Dror, D. M.

2001-01-01

Deficient financing of health services in low-income countries and the absence of universal insurance coverage leaves most of the informal sector in medical indigence, because people cannot assume the financial consequences of illness. The role of communities in solving this problem has been recognized, and many initiatives are under way. However, community financing is rarely structured as health insurance. Communities that pool risks (or offer insurance) have been described as micro-insurance units. The sources of their financial instability and the options for stabilization are explained. Field data from Uganda and the Philippines, as well as simulated situations, are used to examine the arguments. The article focuses on risk transfer from micro-insurance units to reinsurance. The main insight of the study is that when the financial results of micro-insurance units can be estimated, they can enter reinsurance treaties and be stabilized from the first year. The second insight is that the reinsurance pool may require several years of operation before reaching cost neutrality. PMID:11477971

A parallel strategy for predicting the secondary structure of polycistronic microRNAs.

PubMed

Han, Dianwei; Tang, Guiliang; Zhang, Jun

2013-01-01

The biogenesis of a functional microRNA is largely dependent on the secondary structure of the microRNA precursor (pre-miRNA). Recently, it has been shown that microRNAs are present in the genome as the form of polycistronic transcriptional units in plants and animals. It will be important to design efficient computational methods to predict such structures for microRNA discovery and its applications in gene silencing. In this paper, we propose a parallel algorithm based on the master-slave architecture to predict the secondary structure from an input sequence. We conducted some experiments to verify the effectiveness of our parallel algorithm. The experimental results show that our algorithm is able to produce the optimal secondary structure of polycistronic microRNAs.

Evaluating the efficiency of humic acid to remove micro-organisms from denture base material.

PubMed

Meriç, Gökçe; Güvenir, Meryem; Süer, Kaya

2016-09-01

To evaluate the efficiency of humic acid substances on removing micro-organisms from denture base materials. Old denture wearer needs effective, easy-use and safe denture-cleaning material. Square-shaped, heat-polymerised acrylic resin specimens (n = 550) were prepared and divided into five groups (n = 110 for each) corresponding to the microbial contamination (Candida albicans, Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Pseudomonas aeruginosa). Contaminated specimens were randomly assigned to the application of five different denture-cleaning agents as follows (n = 20 for each): Kloroben, Corsodyl, Steradent, Corega, experimental solution with humic acid. Ten specimens were assessed as an experimental control carried out simultaneously for the treatment groups for each micro-organism. It was divided into two groups: negative control and positive control (n = 5 for each). All acrylic specimens were incubated 37°C for 24 h (for bacterial strains) and 37°C for 48 h (for yeast strains). After incubation period, all brain-heart infusion broths (BHI) which contain disinfectant acrylic specimens were cultured on 5% sheep blood agar (for bacteria) and Sabouraud dextrose agar (SDA) for yeast using loop. The numbers of colony-forming units per millilitre (CFU/ml) were calculated. The results were analysed by Mann-Whitney U-test and Kruskal-Wallis tests (p = 0.05). Corsodyl and Kloroben completely eliminated the adherence of all investigated micro-organisms (100%) and showed the highest removal activity compared with other cleaning agents (p < 0.05). There was no statistically significant difference between Corsodyl and Kloroben (p ≥ 0.05), and there was no statistically significant difference between Corega, Steradent and experimental solution (p ≥ 0.05). Humic acid could be used as an alternative 'natural' solution for denture-cleaning agent. © 2014 John Wiley & Sons A/S and The Gerodontology Association. Published by John Wiley & Sons Ltd.

Steady-state [Ca2+]i-force relationship in intact twitching cardiac muscle: direct evidence for modulation by isoproterenol and EMD 53998.

PubMed Central

Dobrunz, L E; Backx, P H; Yue, D T

1995-01-01

We have developed a novel method for measuring steady-state force-[Ca2+]i relations in isolated, membrane-intact rat trabeculae that are microinjected with Fura-2 salt. Twitches are markedly slowed after inhibition of phasic Ca2+ release and uptake from the sarcoplasmic reticulum by addition of cyclopiazonic acid and ryanodine. During relaxation of slowed twitches, force and [Ca2+]i trace a common trajectory in plots of force versus [Ca2+]i, despite very different histories of contraction. The common trajectory thereby provides a high resolution determination of the steady-state relation between force and [Ca2+]i. Using this method, we show that 1 microM isoproterenol, a beta-adrenergic agonist, causes a rightward shift (Hill function K1/2 increased from 0.39 +/- 0.07 microM to 0.82 +/- 0.23 microM, p < 0.02, n = 6) and a decreased slope (nH decreased from 5.4 +/- 1.1 to 4.0 +/- 1.4, p < 0.02) of the steady-state force-[Ca2+]i curve, with no change in maximal force (Fmax = 99.2 +/- 2.2% of control). In contrast, 2 microM EMD 53998, a racemic thiadiazinone derivative, causes a leftward shift (K1/2 decreased from 0.42 +/- 0.02 microM to 0.30 +/- 0.06 microM, p < 0.02, n = 4) with no change in slope of the steady-state force-[Ca2+]i curve, accompanied by a modest increase in maximal force (Fmax = 107.1 +/- 4.6% of control, p < 0.02). To gain mechanistic insight into these modulatory events, we developed a simple model of cooperative thin filament activation that predicts steady-state force-[Ca2+]i relationships. Model analysis suggests that isoproterenol decreases cooperativity arising from nearest-neighbor interactions between regulatory units on the thin filament, without change in the equilibrium constant for Ca2+ binding. In contrast, the effects of EMD 53998 are consistent with an increase in the affinity of strong-binding cross-bridges, without change in either the affinity of troponin C for Ca2+ or cooperative interactions. PMID:7669896

Generation of Micropatterned Substrates Using Micro Photopatterning

PubMed Central

Doyle, Andrew D.

2010-01-01

Micro photopatterning (µPP) has been developed to rapidly test and generate different patterns for extracellular matrix adsorption without being hindered with the process of making physical stamps through nanolithography techniques. It uses two-photon excitation guided through a point-scanning confocal microscope to locally photoablate poly(vinyl) alcohol (PVA) thin films in user-defined computer-controlled patterns. PVA thin films are ideal for surface blocking, being hydrophilic substrates that deter protein adsorption and cell attachment. Because gold substrates are not used during µPP, all live-cell fluorescent imaging techniques including total internal reflection fluorescence microscopy of GFP–linked proteins can be performed with minimal loss of fluorescence signal. Furthermore, because µPP does not require physical stamps for pattern generation, multiple ECMs or other proteins can be localized within microns of each other. This unit details the setup of µPP as well as giving troubleshooting techniques. PMID:20013752

Simulation Based Exploration of Critical Zone Dynamics in Intensively Managed Landscapes

NASA Astrophysics Data System (ADS)

Kumar, P.

2017-12-01

The advent of high-resolution measurements of topographic and (vertical) vegetation features using areal LiDAR are enabling us to resolve micro-scale ( 1m) landscape structural characteristics over large areas. Availability of hyperspectral measurements is further augmenting these LiDAR data by enabling the biogeochemical characterization of vegetation and soils at unprecedented spatial resolutions ( 1-10m). Such data have opened up novel opportunities for modeling Critical Zone processes and exploring questions that were not possible before. We show how an integrated 3-D model at 1m grid resolution can enable us to resolve micro-topographic and ecological dynamics and their control on hydrologic and biogeochemical processes over large areas. We address the computational challenge of such detailed modeling by exploiting hybrid CPU and GPU computing technologies. We show results of moisture, biogeochemical, and vegetation dynamics from studies in the Critical Zone Observatory for Intensively managed Landscapes (IMLCZO) in the Midwestern United States.

Electrostatic Accelerometer for the Gravity Recovery and Climate Experiment Follow-On Mission (GRACE FO)

NASA Astrophysics Data System (ADS)

Perrot, Eddy; Christophe, Bruno; Foulon, Bernard; Boulanger, Damien; Liorzou, Françoise; Lebat, Vincent

2013-04-01

The GRACE FO mission, led by the JPL (Jet Propulsion Laboratory), is an Earth-orbiting gravity mission, continuation of the GRACE mission, that will produce an accurate model of the Earth's gravity field variation providing global climatic data during five year at least. The mission involves two satellites in a loosely controlled tandem formation, with a micro-wave link measuring the inter-satellites distance variation. Non-uniformities in the distribution of the Earth's mass cause the distance between the two satellites to vary. This variation is measured to recover gravity, after substracting the non-gravitational contributors, as the residual drag. ONERA (the French Aerospace Lab) is developing and manufacturing electrostatic accelerometers measuring this residual drag applied on the satellites. The accelerometer is composed of two main parts: the Sensor Unit (including the Sensor Unit Mechanics and the Front-End Electronic Unit) and the Interface Control Unit. In the Accelerometer Core, located in the Sensor Unit Mechanics, the proof mass is levitated and maintained in a center of an electrode cage by electrostatic forces. Thus, any drag acceleration applied on the satellite involves a variation on the servo-controlled electrostatic suspension of the mass. The voltage on the electrodes providing this electrostatic force is the measurement output of the accelerometer. The impact of the accelerometer defaults (geometry, electronic and parasitic forces) leads to bias, misalignment and scale factor error, non-linearity and noise. Some of these accelerometer defaults are characterized by tests with micro-gravity pendulum bench and with drops in ZARM catapult. Besides, a thermal stability is needed for the accelerometer core and front-end electronics to avoid bias and scale factor variation. To reach this stability, the sensor unit is enclosed in a thermal box designed by Astrium, spacecraft manufacturer. The accelerometers are designed to endure mechanical excitation especially due to launching vibrations. As the measure must be accurate, no displacements or sliding must appear during excitations. The electrode cage is made of glass material (ULE), which is very critical, in particular due to the free motion of the proof-mass during the launch. Specific analysis on this part is realized to ensure mechanical behavior. The design of electrostatic accelerometer of the GRACE Follow-On mission benefits of the GRACE heritage, GOCE launched in 2009 and MICROSCOPE which will be launched in 2016, including some improvement to win in performance, in particular the thermal sensitivity of the measurements.

Non-lobar atelectasis generates inflammation and structural alveolar injury in the surrounding healthy tissue during mechanical ventilation.

PubMed

Retamal, Jaime; Bergamini, Bruno Curty; Carvalho, Alysson R; Bozza, Fernando A; Borzone, Gisella; Borges, João Batista; Larsson, Anders; Hedenstierna, Göran; Bugedo, Guillermo; Bruhn, Alejandro

2014-09-09

When alveoli collapse the traction forces exerted on their walls by adjacent expanded units may increase and concentrate. These forces may promote its re-expansion at the expense of potentially injurious stresses at the interface between the collapsed and the expanded units. We developed an experimental model to test the hypothesis that a local non-lobar atelectasis can act as a stress concentrator, contributing to inflammation and structural alveolar injury in the surrounding healthy lung tissue during mechanical ventilation. A total of 35 rats were anesthetized, paralyzed and mechanically ventilated. Atelectasis was induced by bronchial blocking: after five minutes of stabilization and pre-oxygenation with FIO2 = 1.0, a silicon cylinder blocker was wedged in the terminal bronchial tree. Afterwards, the animals were randomized between two groups: 1) Tidal volume (VT) = 10 ml/kg and positive end-expiratory pressure (PEEP) = 3 cmH2O (VT10/PEEP3); and 2) VT = 20 ml/kg and PEEP = 0 cmH2O (VT20/zero end-expiratory pressure (ZEEP)). The animals were then ventilated during 180 minutes. Three series of experiments were performed: histological (n = 12); tissue cytokines (n = 12); and micro-computed tomography (microCT; n = 2). An additional six, non-ventilated, healthy animals were used as controls. Atelectasis was successfully induced in the basal region of the lung of 26 out of 29 animals. The microCT of two animals revealed that the volume of the atelectasis was 0.12 and 0.21 cm3. There were more alveolar disruption and neutrophilic infiltration in the peri-atelectasis region than the corresponding contralateral lung (control) in both groups. Edema was higher in the peri-atelectasis region than the corresponding contralateral lung (control) in the VT20/ZEEP than VT10/PEEP3 group. The volume-to-surface ratio was higher in the peri-atelectasis region than the corresponding contralateral lung (control) in both groups. We did not find statistical difference in tissue interleukin-1β and cytokine-induced neutrophil chemoattractant-1 between regions. The present findings suggest that a local non-lobar atelectasis acts as a stress concentrator, generating structural alveolar injury and inflammation in the surrounding lung tissue.

Design of Architectures and Materials in In-Plane Micro-supercapacitors: Current Status and Future Challenges.

PubMed

Qi, Dianpeng; Liu, Yan; Liu, Zhiyuan; Zhang, Li; Chen, Xiaodong

2017-02-01

The rapid development of integrated electronics and the boom in miniaturized and portable devices have increased the demand for miniaturized and on-chip energy storage units. Currently thin-film batteries or microsized batteries are commercially available for miniaturized devices. However, they still suffer from several limitations, such as short lifetime, low power density, and complex architecture, which limit their integration. Supercapacitors can surmount all these limitations. Particularly for micro-supercapacitors with planar architectures, due to their unique design of the in-plane electrode finger arrays, they possess the merits of easy fabrication and integration into on-chip miniaturized electronics. Here, the focus is on the different strategies to design electrode finger arrays and the material engineering of in-plane micro-supercapacitors. It is expected that the advances in micro-supercapacitors with in-plane architectures will offer new opportunities for the miniaturization and integration of energy-storage units for portable devices and on-chip electronics. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Generation of Well-Defined Micro/Nanoparticles via Advanced Manufacturing Techniques for Therapeutic Delivery

PubMed Central

Zhang, Peipei; Xia, Junfei; Luo, Sida

2018-01-01

Micro/nanoparticles have great potentials in biomedical applications, especially for drug delivery. Existing studies identified that major micro/nanoparticle features including size, shape, surface property and component materials play vital roles in their in vitro and in vivo applications. However, a demanding challenge is that most conventional particle synthesis techniques such as emulsion can only generate micro/nanoparticles with a very limited number of shapes (i.e., spherical or rod shapes) and have very loose control in terms of particle sizes. We reviewed the advanced manufacturing techniques for producing micro/nanoparticles with precisely defined characteristics, emphasizing the use of these well-controlled micro/nanoparticles for drug delivery applications. Additionally, to illustrate the vital roles of particle features in therapeutic delivery, we also discussed how the above-mentioned micro/nanoparticle features impact in vitro and in vivo applications. Through this review, we highlighted the unique opportunities in generating controllable particles via advanced manufacturing techniques and the great potential of using these micro/nanoparticles for therapeutic delivery. PMID:29670013

A three-level support method for smooth switching of the micro-grid operation model

NASA Astrophysics Data System (ADS)

Zong, Yuanyang; Gong, Dongliang; Zhang, Jianzhou; Liu, Bin; Wang, Yun

2018-01-01

Smooth switching of micro-grid between the grid-connected operation mode and off-grid operation mode is one of the key technologies to ensure it runs flexible and efficiently. The basic control strategy and the switching principle of micro-grid are analyzed in this paper. The reasons for the fluctuations of the voltage and the frequency in the switching process are analyzed from views of power balance and control strategy, and the operation mode switching strategy has been improved targeted. From the three aspects of controller’s current inner loop reference signal, voltage outer loop control strategy optimization and micro-grid energy balance management, a three-level security strategy for smooth switching of micro-grid operation mode is proposed. From the three aspects of controller’s current inner loop reference signal tracking, voltage outer loop control strategy optimization and micro-grid energy balance management, a three-level strategy for smooth switching of micro-grid operation mode is proposed. At last, it is proved by simulation that the proposed control strategy can make the switching process smooth and stable, the fluctuation problem of the voltage and frequency has been effectively improved.

Data supporting attempted caveolae-mediated phagocytosis of surface-fixed micro-pillars by human osteoblasts.

PubMed

Moerke, Caroline; Mueller, Petra; Nebe, Barbara

2016-06-01

The provided data contains the phagocytic interaction of human MG-63 osteoblasts with micro-particles 6 µm in size as well as geometric micro-pillared topography with micro-pillar sizes 5 µm of length, width, height and spacing respectively related to the research article entitled "Attempted caveolae-mediated phagocytosis of surface-fixed micro-pillars by human osteoblasts" in the Biomaterials journal. [1] Micro-particle treatment was used as positive control triggering phagocytosis by the osteoblasts. Caveolin-1 (Cav-1) as major structural component of caveolae [2] plays an important role in the phagocytic process of micro-particles and -pillars. Data related to the experiments in [1] with siRNA-mediated knockdown are presented here as well as micro-particle control experiments, tubulin analysis on the micro-pillared topography and initial cell interaction with the micro-pillars.

MicroRNA Expression Profiles as Biomarkers of Minor Salivary Gland Inflammation and Dysfunction in Sjögren's Syndrome

PubMed Central

Alevizos, Ilias; Alexander, Stefanie; Turner, R. James; Illei, Gabor G.

2013-01-01

Objective MicroRNA reflect physiologic and pathologic processes and may be used as biomarkers of concurrent pathophysiologic events in complex settings such as autoimmune diseases. We generated microRNA microarray profiles from the minor salivary glands of control subjects without Sjögren's syndrome (SS) and patients with SS who had low-grade or high-grade inflammation and impaired or normal saliva production, to identify microRNA patterns specific to salivary gland inflammation or dysfunction. Methods MicroRNA expression profiles were generated by Agilent microRNA arrays. We developed a novel method for data normalization by identifying housekeeping microRNA. MicroRNA profiles were compared by unsupervised mathematical methods to test how well they distinguish between control subjects and various subsets of patients with SS. Several bioinformatics methods were used to predict the messenger RNA targets of the differentially expressed microRNA. Results MicroRNA expression patterns accurately distinguished salivary glands from control subjects and patients with SS who had low-degree or high-degree inflammation. Using real-time quantitative polymerase chain reaction, we validated 2 microRNA as markers of inflammation in an independent cohort. Comparing microRNA from patients with preserved or low salivary flow identified a set of differentially expressed microRNA, most of which were up-regulated in the group with decreased salivary gland function, suggesting that the targets of microRNA may have a protective effect on epithelial cells. The predicted biologic targets of microRNA associated with inflammation or salivary gland dysfunction identified both overlapping and distinct biologic pathways and processes. Conclusion Distinct microRNA expression patterns are associated with salivary gland inflammation and dysfunction in patients with SS, and microRNA represent a novel group of potential biomarkers. PMID:21280008

The MicroMAS CubeSat Mission

NASA Astrophysics Data System (ADS)

Cahoy, K.; Blackwell, W. J.; Allen, G.; Bury, M.; Efromson, R.; Galbraith, C.; Hancock, T.; Leslie, V.; Osaretin, I.; Retherford, L.; Scarito, M.; Shields, M.; Toher, D.; Wight, K.; Miller, D.; Marinan, A.; Paek, S.; Peters, E.; Schmidt, F. H.; Alvisio, B.; Wise, E.; Masterson, R.; Franzim Miranda, D.; Crail, C.; Kingsbury, R.; Souffrant, A.; Orrego, L.; Eslinger, G.; Nicholas, A.; Pong, C.

2012-12-01

The recently published Midterm Assessment of NASA's Implementation of the Decadal Survey finds that, "The nation's Earth observing system is beginning a rapid decline in capability as long-running missions end and key new missions are delayed, lost, or canceled. The projected loss of observing capability could have significant adverse consequences for science and society." In this presentation, we explore low-cost, mission-flexible, and rapidly deployable spaceborne sensors that can meet stringent performance requirements pervading the NASA Earth Science measurement programs, including especially the recommended NRC Decadal Survey missions. New technologies have enabled a novel approach toward this science observational goal, and in this paper we describe recent technology develop efforts to address the challenges above through the use of CubeSat radiometers. The Micro-sized Microwave Atmospheric Satellite (MicroMAS) is a 3U cubesat (30x10x10 cm, ~4kg) hosting a passive microwave spectrometer operating near the 118.75-GHz oxygen absorption line. The focus of the first MicroMAS mission (hereafter, MicroMAS-1) is to observe convective thunderstorms, tropical cyclones, and hurricanes from a near-equatorial orbit at approximately 500-km altitude. A MicroMAS flight unit is currently being developed in anticipation of a 2014 launch to be provided by NASA. A parabolic reflector is mechanically rotated as the spacecraft orbits the earth, thus directing a cross-track scanned beam with FWHM beamwidth of 2.4-degrees, yielding an approximately 25-km diameter footprint from a nominal altitude of 500 km. Radiometric calibration is carried out using observations of cold space, the earth's limb, and an internal noise diode that is weakly coupled through the RF front-end electronics. A key technology feature is the development of an ultra-compact intermediate frequency processor module for channelization, detection, and A-to-D conversion. The antenna system and RF front-end electronics are highly integrated and miniaturized. A MicroMAS-2 mission is currently being planned using a multi-band spectrometer operating near 118 and 183 GHz in a sun-synchronous orbit of approximately 800-km altitude. A HyMAS-1 (Hyperspectral Microwave Atmospheric Satellite) mission with approximately 50 channels near 118 and 183 GHz is also being planned. In this talk, the mission concept of operations will be discussed, the radiometer payload will be described, and the spacecraft subsystems (avionics, power, communications, attitude determination and control, and mechanical structures) will be summarized. Test data from the recently completed MicroMAS Engineering Development Model (EDM) will also be presented. This work was sponsored by the National Oceanic and Atmospheric Administration under Air Force contract FA8721-05-C-0002. Opinions, interpretations, conclusions, and recommendations are those of the authors and not necessarily endorsed by the United States Government.

Evaluation of the Microsemi CRP, an automated hematology analyzer for rapid 3-part WBC differential and CRP using whole blood.

PubMed

Nomura, N; Saito, K; Ikeda, M; Yuasa, S; Pastore, M; Chabert, C; Kono, E; Sakai, A; Tanaka, H; Ikemoto, T; Takubo, T

2015-08-01

We evaluated the basic performance of Microsemi CRP, an unique automated hematology analyzer which can simultaneously measure CBC including 3-part WBC differential (3-Diff) and CRP using whole blood treated with EDTA-2K anticoagulant. We found that it produced generally the acceptable results for all parameters performed (repeatability, reproducibility, linearity, interference effect, carry over, and correlation) using control materials, fresh human whole bloods, and serum samples. CBC data examined using Microsemi CRP showed the good correlation with the previous model, Micros CRP200 (r ≧ 0.9), and also those obtained using the routine analyzer, ADVIA 2120i (r ≧ 0.989). Concerning the 3-Diff, both GRA (%) and LYM (%) showed the excellent correlation coefficient between Microsemi CRP and Micros CRP200 (r ≧ 0.992) as well as ADVIA 2120i (r ≧ 0.957). MON (%) showed good correlation between Microsemi CRP and Micros CRP200 (r = 0.959), but lower correlation between Microsemi CRP and ADVIA 2120 i (r = 0.471). CRP data showed the good correlation with HITACHI7600 (r ≧ 0.997) and Micros CRP200 (r ≧ 0.997). From these findings, we concluded that Microsemi CRP seemed the convenient laboratory analyzer in the setting of point of care testing (POCT) especially at NICU or primary care unit. © 2014 John Wiley & Sons Ltd.

Shaping electromagnetic waves using software-automatically-designed metasurfaces.

PubMed

Zhang, Qian; Wan, Xiang; Liu, Shuo; Yuan Yin, Jia; Zhang, Lei; Jun Cui, Tie

2017-06-15

We present a fully digital procedure of designing reflective coding metasurfaces to shape reflected electromagnetic waves. The design procedure is completely automatic, controlled by a personal computer. In details, the macro coding units of metasurface are automatically divided into several types (e.g. two types for 1-bit coding, four types for 2-bit coding, etc.), and each type of the macro coding units is formed by discretely random arrangement of micro coding units. By combining an optimization algorithm and commercial electromagnetic software, the digital patterns of the macro coding units are optimized to possess constant phase difference for the reflected waves. The apertures of the designed reflective metasurfaces are formed by arranging the macro coding units with certain coding sequence. To experimentally verify the performance, a coding metasurface is fabricated by automatically designing two digital 1-bit unit cells, which are arranged in array to constitute a periodic coding metasurface to generate the required four-beam radiations with specific directions. Two complicated functional metasurfaces with circularly- and elliptically-shaped radiation beams are realized by automatically designing 4-bit macro coding units, showing excellent performance of the automatic designs by software. The proposed method provides a smart tool to realize various functional devices and systems automatically.

Microfabrication and Test of a Three-Dimensional Polymer Hydro-focusing Unit for Flow Cytometry Applications

NASA Technical Reports Server (NTRS)

Yang, Ren; Feeback, Daniel L.; Wang, Wan-Jun

2005-01-01

This paper details a novel three-dimensional (3D) hydro-focusing micro cell sorter for micro flow cytometry applications. The unit was microfabricated by means of SU-8 3D lithography. The 3D microstructure for coaxial sheathing was designed, microfabricated, and tested. Three-dimensional hydrofocusing capability was demonstrated with an experiment to sort labeled tanned sheep erythrocytes (red blood cells). This polymer hydro-focusing microstructure is easily microfabricated and integrated with other polymer microfluidic structures. Keywords: SU-8, three-dimensional hydro-focusing, microfluidic, microchannel, cytometer

a New Approach for Complete Mixing by Transverse and Streamwise Flow Motions in Micro-Channels

NASA Astrophysics Data System (ADS)

Wang, Muh-Rong; Dai, Chiau-Yi; Huang, Yang-Sheng

Mixing control is an important issue in micro-fluid chip applications, such as μTAS (Micro-Total Analysis System) or LOC (Lab-on-Chip) because the flow at micro-scale is highly laminar. Several flow control schemes had been developed for complete mixing in the micro-channels in the past decades. However, most of the mixing control schemes are performed by utilizing specific excitation devices, such as electrokinetic, magnetic or pressure drivers. This paper investigates a new control scheme which is composed of a series of flow manipulation by changing the pressure at the two inlets of the micromixer as the external excitation. The fluids from two inlets are introduced to a square mixing chamber, which provides a space where the streamwise and transverse flow motions take place. The results show that the micromixer can be used to produce a large recirculation zone with series of small transverse fringes under external excitations. It is found that this new flow pattern enhances mixing processes at the micro-scale. A complete mixing can be achieved under appropriate flow control with the corresponding design.

Identification of suitable reference genes for hepatic microRNA quantitation.

PubMed

Lamba, Vishal; Ghodke-Puranik, Yogita; Guan, Weihua; Lamba, Jatinder K

2014-03-07

MicroRNAs (miRNAs) are short (~22 nt) endogenous RNAs that play important roles in regulating expression of a wide variety of genes involved in different cellular processes. Alterations in microRNA expression patterns have been associated with a number of human diseases. Accurate quantitation of microRNA levels is important for their use as biomarkers and in determining their functions. Real time PCR is the gold standard and the most frequently used technique for miRNA quantitation. Real time PCR data analysis includes normalizing the amplification data to suitable endogenous control/s to ensure that microRNA quantitation is not affected by the variability that is potentially introduced at different experimental steps. U6 (RNU6A) and RNU6B are two commonly used endogenous controls in microRNA quantitation. The present study was designed to investigate inter-individual variability and gender differences in hepatic microRNA expression as well as to identify the best endogenous control/s that could be used for normalization of real-time expression data in liver samples. We used Taqman based real time PCR to quantitate hepatic expression levels of 22 microRNAs along with U6 and RNU6B in 50 human livers samples (25 M, 25 F). To identify the best endogenous controls for use in data analysis, we evaluated the amplified candidates for their stability (least variability) in expression using two commonly used software programs: Normfinder and GeNormplus, Both Normfinder and GeNormplus identified U6 to be among the least stable of all the candidates analyzed, and RNU6B was also not among the top genes in stability. mir-152 and mir-23b were identified to be the two most stable candidates by both Normfinder and GeNormplus in our analysis, and were used as endogenous controls for normalization of hepatic miRNA levels. Measurements of microRNA stability indicate that U6 and RNU6B are not suitable for use as endogenous controls for normalizing microRNA relative quantitation data in hepatic tissue, and their use can led to possibly erroneous conclusions.

Note: A resonating reflector-based optical system for motion measurement in micro-cantilever arrays

DOE Office of Scientific and Technical Information (OSTI.GOV)

Sathishkumar, P.; Punyabrahma, P.; Sri Muthu Mrinalini, R.

A robust, compact optical measurement unit for motion measurement in micro-cantilever arrays enables development of portable micro-cantilever sensors. This paper reports on an optical beam deflection-based system to measure the deflection of micro-cantilevers in an array that employs a single laser source, a single detector, and a resonating reflector to scan the measurement laser across the array. A strategy is also proposed to extract the deflection of individual cantilevers from the acquired data. The proposed system and measurement strategy are experimentally evaluated and demonstrated to measure motion of multiple cantilevers in an array.

A Force-Controllable Macro-Micro Manipulator and its Application to Medical Robotics

NASA Technical Reports Server (NTRS)

Marzwell, Neville I.; Uecker, Darrin R.; Wang, Yulun

1993-01-01

This paper describes an 8-degrees-of-freedom macro-micro robot. This robot is capable of performing tasks that require accurate force control, such as polishing, finishing, grinding, deburring, and cleaning. The design of the macro-micro mechanism, the control algorithms, and the hardware/sofware implemtation of the algotithms are described in this paper. Initial experimental results are reported.

Microvibration and Centre-of-Gravity Shift Measurements on Thermally Stressed Thermal-Control Blankets

NASA Astrophysics Data System (ADS)

Magg, Manfred; Grillenbeck, Anton, , Dr.

2004-08-01

Several samples of thermal control blankets were subjected to transient thermal loads in a thermal vacuum chamber in order to study their ability to excite micro- vibrations on a carrier structure and to cause tiny centre- of-gravity shifts. The reason for this investigation was driven by the GOCE project in order to minimize micro- vibrations on-board of the spacecraft while on-orbit. The objectives of this investigation were to better understand the mechanism which may produce micro- vibrations induced by the thermal control blankets, and to identify thermal control blanket lay-ups with minimum micro-vibration activity.

Selective cyclopalladation of R3P=NCH2Aryl iminophosphoranes. Experimental and computational study.

PubMed

Bielsa, Raquel; Navarro, Rafael; Urriolabeitia, Esteban P; Lledós, Agustí

2007-11-26

The orientation of the orthopalladation of iminophosphoranes R3P=NCH2Aryl (R=Ph, Aryl=Ph (1a), C6H(4)-2-Br (1b), C6H4-Me-2 (1e), C6H3-(Me)(2)-2,5 (1f); R=p-tolyl, Aryl=Ph (1c); R=m-tolyl, Aryl=Ph (1d); R3P=MePh2P, and Aryl=Ph (1g)) has been studied. 1a reacts with Pd(OAc)2 (OAc=acetate) giving endo-[Pd(micro-Cl){C,N-C6H4(PPh2=NCH2Ph)-2}]2 (3a), while exo-[Pd(micro-Br){C,N-C6H4(CH2N=PPh3)-2}]2 (3b) could only be obtained by the oxidative addition of 1b to Pd2(dba)3. The endo form of the metalated ligand is favored kinetically and thermodynamically, as shown by the conversion of exo-[Pd(micro-OAc){C,N-C6H4(CH2N=PPh3)-2}]2 (2b) into endo-[Pd(micro-OAc){C,N-C6H4(PPh2=NCH2Ph)-2}]2 (2a) in refluxing toluene. The orientation of the reaction is not affected by the introduction of electron-releasing substituents at the Ph rings of the PR3 (1c and 1d) or the benzyl units (1e and 1f), and endo complexes (3c-3f) were obtained in all cases. The palladation of MePh2P=NCH2Ph (1g) can be regioselectively oriented as a function of the solvent. The exo isomer [Pd(micro-Cl){C6H4(CH2N=PPh2Me)-2}]2 (exo-3g) is obtained in refluxing CH2Cl2, while endo-[Pd(micro-Cl){C,N-C6H4(PPh(Me)=NCH2Ph)-2}]2 (endo-3g) can be isolated as a single isomer in refluxing toluene. In this case, the exo metalation is kinetically favored while an endo process occurs under thermodynamic control, as shown through the rearrangement of [Pd(micro-OAc){C6H4(CH2N=PPh2Me)-2}]2 (exo-2g) into [Pd(micro-OAc){C,N-C6H4(P(Ph)Me=NCH2Ph)-2}]2 (endo-2g) in refluxing toluene. The preference for the endo palladation of 1a and the kinetic versus thermodynamic control in 1g has been explained through DFT studies of the reaction mechanism.

Experimental study on line-of-sight (LOS) attitude control using control moment gyros under micro-gravity environment

NASA Astrophysics Data System (ADS)

Kojima, Hirohisa; Hiraiwa, Kana; Yoshimura, Yasuhiro

2018-02-01

This paper presents the results of line-of-sight (LOS) attitude control using control moment gyros under a micro-gravity environment generated by parabolic flight. The W-Z parameters are used to describe the spacecraft attitude. In order to stabilize the current LOS to the target LOS, backstepping-based feedback control is considered using the W-Z parameters. Numerical simulations and experiments under a micro-gravity environment are carried out, and their results are compared in order to validate the proposed control methods.

A macro-micro robot for precise force applications

NASA Technical Reports Server (NTRS)

Marzwell, Neville I.; Wang, Yulun

1993-01-01

This paper describes an 8 degree-of-freedom macro-micro robot capable of performing tasks which require accurate force control. Applications such as polishing, finishing, grinding, deburring, and cleaning are a few examples of tasks which need this capability. Currently these tasks are either performed manually or with dedicated machinery because of the lack of a flexible and cost effective tool, such as a programmable force-controlled robot. The basic design and control of the macro-micro robot is described in this paper. A modular high-performance multiprocessor control system was designed to provide sufficient compute power for executing advanced control methods. An 8 degree of freedom macro-micro mechanism was constructed to enable accurate tip forces. Control algorithms based on the impedance control method were derived, coded, and load balanced for maximum execution speed on the multiprocessor system.

Study on the Automatic Detection Method and System of Multifunctional Hydrocephalus Shunt

NASA Astrophysics Data System (ADS)

Sun, Xuan; Wang, Guangzhen; Dong, Quancheng; Li, Yuzhong

2017-07-01

Aiming to the difficulty of micro pressure detection and the difficulty of micro flow control in the testing process of hydrocephalus shunt, the principle of the shunt performance detection was analyzed.In this study, the author analyzed the principle of several items of shunt performance detection,and used advanced micro pressure sensor and micro flow peristaltic pump to overcome the micro pressure detection and micro flow control technology.At the same time,This study also puted many common experimental projects integrated, and successfully developed the automatic detection system for a shunt performance detection function, to achieve a test with high precision, high efficiency and automation.

Acoustic trapping in bubble-bounded micro-cavities

NASA Astrophysics Data System (ADS)

O'Mahoney, P.; McDougall, C.; Glynne-Jones, P.; MacDonald, M. P.

2016-12-01

We present a method for controllably producing longitudinal acoustic trapping sites inside microfluidic channels. Air bubbles are injected into a micro-capillary to create bubble-bounded `micro-cavities'. A cavity mode is formed that shows controlled longitudinal acoustic trapping between the two air/water interfaces along with the levitation to the centre of the channel that one would expect from a lower order lateral mode. 7 μm and 10 μm microspheres are trapped at the discrete acoustic trapping sites in these micro-cavities.We show this for several lengths of micro-cavity.

Fast, externally triggered, digital phase controller for an optical lattice

NASA Astrophysics Data System (ADS)

Sadgrove, Mark; Nakagawa, Ken'ichi

2011-11-01

We present a method to control the phase of an optical lattice according to an external trigger signal. The method has a latency of less than 30 μs. Two phase locked digital synthesizers provide the driving signal for two acousto-optic modulators which control the frequency and phase of the counter-propagating beams which form a standing wave (optical lattice). A micro-controller with an external interrupt function is connected to the desired external signal, and updates the phase register of one of the synthesizers when the external signal changes. The standing wave (period λ/2 = 390 nm) can be moved by units of 49 nm with a mean jitter of 28 nm. The phase change is well known due to the digital nature of the synthesizer, and does not need calibration. The uses of the scheme include coherent control of atomic matter-wave dynamics.

Design and Control of Flapping Wing Micro Air Vehicles

DTIC Science & Technology

2011-09-01

unsteady, low Re aerodynamics, micro-fabrication, and fluid - structure interaction. However, research into flapping wing control of such MAVs...and flown in confined spaces such as urban canyons, caves and indoors. MAVs will provide an organic ISR capability to small combat teams in the...Designing for highly coupled fluid -structure interactions  Micro-fabrication  Stability characterization and control Of these challenges, the most

A model for the Global Quantum Efficiency for a TPB-based wavelength-shifting system used with photomultiplier tubes in liquid argon in MicroBooNE

NASA Astrophysics Data System (ADS)

Pate, S. F.; Wester, T.; Bugel, L.; Conrad, J.; Henderson, E.; Jones, B. J. P.; McLean, A. I. L.; Moon, J. S.; Toups, M.; Wongjirad, T.

2018-02-01

We present a model for the Global Quantum Efficiency (GQE) of the MicroBooNE optical units. An optical unit consists of a flat, circular acrylic plate, coated with tetraphenyl butadiene (TPB), positioned near the photocathode of a 20.2-cm diameter photomultiplier tube. The plate converts the ultra-violet scintillation photons from liquid argon into visible-spectrum photons to which the cryogenic phototubes are sensitive. The GQE is the convolution of the efficiency of the plates that convert the 128 nm scintillation light from liquid argon to visible light, the efficiency of the shifted light to reach the photocathode, and the efficiency of the cryogenic photomultiplier tube. We develop a GEANT4-based model of the optical unit, based on first principles, and obtain the range of probable values for the expected number of detected photoelectrons (NPE) given the known systematic errors on the simulation parameters. We compare results from four measurements of the NPE determined using alpha-particle sources placed at two distances from a TPB-coated plate in a liquid argon cryostat test stand. We also directly measured the radial dependence of the quantum efficiency, and find that this has the same shape as predicted by our model. Our model results in a GQE of 0.0055±0.0009 for the MicroBooNE optical units. While the information shown here is MicroBooNE specific, the approach to the model and the collection of simulation parameters will be widely applicable to many liquid-argon-based light collection systems.

A systematic approach to fabricate high aspect ratio silicon micro-needles for transdermal drug delivery

NASA Astrophysics Data System (ADS)

Ng, H. B.; Shearwood, C.

2007-12-01

The successful development of micro-needles can help transport drugs and vaccines both effectively and painlessly across the skin. However, not all micro-needles are strong enough to withstand the insertion forces and viscoelasticity of the skin. The work here focuses on the micro-fabrication of high aspect ratio needles with careful control of needle-profile using dry etching technologies. Silicon micro-needles, 150μm in length with base-diameters ranging from 90 to 240μm have been investigated in this study. A novel, multiple-sacrificial approach has been demonstrated as suited to the fabrication of long micro-needle bodies with positive profiles. The parameters that control the isotropic etching are adjusted to control the ratio of the needle-base diameter to needle length. By careful control of geometry, the needle profile can be engineered to give a suitable tip size for penetration, as well as a broad needle base to facilitate the creation of either single or multiple-through holes. This approach allows the mechanical properties of the otherwise brittle needles to be optimized. Finite element analysis indicates that the micro-needles will fracture prematurely due to buckling, with forces ranging from 10 to 30mN.

Unit cell-based computer-aided manufacturing system for tissue engineering.

PubMed

Kang, Hyun-Wook; Park, Jeong Hun; Kang, Tae-Yun; Seol, Young-Joon; Cho, Dong-Woo

2012-03-01

Scaffolds play an important role in the regeneration of artificial tissues or organs. A scaffold is a porous structure with a micro-scale inner architecture in the range of several to several hundreds of micrometers. Therefore, computer-aided construction of scaffolds should provide sophisticated functionality for porous structure design and a tool path generation strategy that can achieve micro-scale architecture. In this study, a new unit cell-based computer-aided manufacturing (CAM) system was developed for the automated design and fabrication of a porous structure with micro-scale inner architecture that can be applied to composite tissue regeneration. The CAM system was developed by first defining a data structure for the computing process of a unit cell representing a single pore structure. Next, an algorithm and software were developed and applied to construct porous structures with a single or multiple pore design using solid freeform fabrication technology and a 3D tooth/spine computer-aided design model. We showed that this system is quite feasible for the design and fabrication of a scaffold for tissue engineering.

The Optimization dispatching of Micro Grid Considering Load Control

NASA Astrophysics Data System (ADS)

Zhang, Pengfei; Xie, Jiqiang; Yang, Xiu; He, Hongli

2018-01-01

This paper proposes an optimization control of micro-grid system economy operation model. It coordinates the new energy and storage operation with diesel generator output, so as to achieve the economic operation purpose of micro-grid. In this paper, the micro-grid network economic operation model is transformed into mixed integer programming problem, which is solved by the mature commercial software, and the new model is proved to be economical, and the load control strategy can reduce the charge and discharge times of energy storage devices, and extend the service life of the energy storage device to a certain extent.

Operational efficiency and sustainability of vector control of malaria and dengue: descriptive case studies from the Philippines

PubMed Central

2012-01-01

Background Analysis is lacking on the management of vector control systems in disease-endemic countries with respect to the efficiency and sustainability of operations. Methods Three locations were selected, at the scale of province, municipality and barangay (i.e. village). Data on disease incidence, programme activities, and programme management were collected on-site through meetings and focus group discussions. Results Adaptation of disease control strategies to the epidemiological situation per barangay, through micro-stratification, brings gains in efficiency, but should be accompanied by further capacity building on local situational analysis for better selection and targeting of vector control interventions within the barangay. An integrated approach to vector control, aiming to improve the rational use of resources, was evident with a multi-disease strategy for detection and response, and by the use of combinations of vector control methods. Collaboration within the health sector was apparent from the involvement of barangay health workers, re-orientation of job descriptions and the creation of a disease surveillance unit. The engagement of barangay leaders and use of existing community structures helped mobilize local resources and voluntary services for vector control. In one location, local authorities and the community were involved in the planning, implementation and evaluation of malaria control, which triggered local programme ownership. Conclusions Strategies that contributed to an improved efficiency and sustainability of vector control operations were: micro-stratification, integration of vector control within the health sector, a multi-disease approach, involvement of local authorities, and empowerment of communities. Capacity building on situational analysis and vector surveillance should be addressed through national policy and guidelines. PMID:22873707

Interaction of microRNA-21/145 and Smad3 domain-specific phosphorylation in hepatocellular carcinoma

PubMed Central

Wang, Ji Yu; Fang, Meng; Boye, Alex; Wu, Chao; Wu, Jia Jun; Ma, Ying; Hou, Shu; Kan, Yue; Yang, Yan

2017-01-01

MicroRNAs 21 and 145 exhibit inverse expression in Hepatocellular carcinoma (HCC), but how they relate to Smad3 C-terminal and Link region phosphorylation (pSmad3C and pSmad3L) downstream of TGF-β/MAPK signaling, remains inconclusive. Our results suggest microRNA-145 targets Smad3 in HepG2 cells. Decreased tumor volume and increased apoptosis were produced in both microRNA-21 antagomir and microRNA-145 agomir groups compared to controls. Inhibition of TβRI and MAPK (ERK, JNK, and p38) activation respectively produced decreased microRNA-21 but increased microRNA-145 expression. Correspondingly, the expression level of pSmad3C obviously increased while pSmad3L decreased in microRNA-145 agomir-group and the expression of pSmad3C/3L were not markedly changed but pERK, pJNK, pp38 decreased in microRNA-21 antagomir-group compared to controls. On the other hand, microRNA-145 and 21 increased respectively in xenografts of HepG2 cells transfected with Smad3 EPSM and 3S-A plasmid, and this correlated with the overexpression of pSmad3C and pSmad3L respectively compared to control. To conclude, microRNA-21 promotes tumor progression in a MAPK-dependent manner while microRNA-145 suppresses it via domain-specific phosphorylation of Smad3 in HCC. Meanwhile, increased pSmad3C/3L lead to the up-regulation of microRNA-145/21 respectively. The interaction between pSmad3C/3L and microRNA-145/21 regulates HCC progression and the switch of pSmad3C/3L may serve as an important target for HCC therapy. PMID:29156696

A low power flash-FPGA based brain implant micro-system of PID control.

PubMed

Lijuan Xia; Fattah, Nabeel; Soltan, Ahmed; Jackson, Andrew; Chester, Graeme; Degenaar, Patrick

2017-07-01

In this paper, we demonstrate that a low power flash FPGA based micro-system can provide a low power programmable interface for closed-loop brain implant inter- faces. The proposed micro-system receives recording local field potential (LFP) signals from an implanted probe, performs closed-loop control using a first order control system, then converts the signal into an optogenetic control stimulus pattern. Stimulus can be implemented through optoelectronic probes. The long term target is for both fundamental neuroscience applications and for clinical use in treating epilepsy. Utilizing our device, closed-loop processing consumes only 14nJ of power per PID cycle compared to 1.52μJ per cycle for a micro-controller implementation. Compared to an application specific digital integrated circuit, flash FPGA's are inherently programmable.

Religiosity as self-enhancement: a meta-analysis of the relation between socially desirable responding and religiosity.

PubMed

Sedikides, Constantine; Gebauer, Jochen E

2010-02-01

In a meta-analysis, the authors test the theoretical formulation that religiosity is a means for self-enhancement. The authors operationalized self-enhancement as socially desirable responding (SDR) and focused on three facets of religiosity: intrinsic, extrinsic, and religion-as-quest. Importantly, they assessed two moderators of the relation between SDR and religiosity. Macro-level culture reflected countries that varied in degree of religiosity (from high to low: United States, Canada, United Kingdom). Micro-level culture reflected U.S. universities high (Christian) versus low (secular) on religiosity. The results were generally consistent with the theoretical formulation. Both macro-level and micro-level culture moderated the relation between SDR and religiosity: This relation was more positive in samples that placed higher value on religiosity (United States > Canada > United Kingdom; Christian universities > secular universities). The evidence suggests that religiosity is partly in the service of self-enhancement.

Micro guidance and control synthesis: New components, architectures, and capabilities

NASA Technical Reports Server (NTRS)

Mettler, Edward; Hadaegh, Fred Y.

1993-01-01

New GN&C (guidance, navigation and control) system capabilities are shown to arise from component innovations that involve the synergistic use of microminiature sensors and actuators, microelectronics, and fiber optics. Micro-GN&C system and component concepts are defined that include micro-actuated adaptive optics, micromachined inertial sensors, fiber-optic data nets and light-power transmission, and VLSI microcomputers. The thesis is advanced that these micro-miniaturization products are capable of having a revolutionary impact on space missions and systems, and that GN&C is the pathfinder micro-technology application that can bring that about.

Application of a hierarchical habitat unit classification system: stream habitat and salmonid distribution in Ward Creek, southeast Alaska.

Treesearch

M.D. Bryant; B.E. Wright; B.J. Davies

1992-01-01

A hierarchical classification system separating stream habitat into habitat units defined by stream morphology and hydrology was used in a pre-enhancement stream survey. The system separates habitat units into macrounits, mesounits, and micro- units and includes a separate evaluation of instream cover that also uses the hierarchical scheme. This paper presents an...

Application of Micro-ramp Flow Control Devices to an Oblique Shock Interaction

NASA Technical Reports Server (NTRS)

Hirt, Stefanie; Anderson, Bernhard

2007-01-01

Tests are planned in the 15cm x 15cm supersonic wind tunnel at NASA Glenn to demonstrate the applicability of micro-ramp flow control to the management of shock wave boundary layer interactions. These tests will be used as a database for computational fluid dynamics (CFD) validation and Design of Experiments (DoE) design information. Micro-ramps show potential for mechanically simple and fail-safe boundary layer control.

Circulating cell-derived microparticles in patients with minimally symptomatic obstructive sleep apnoea.

PubMed

Ayers, L; Ferry, B; Craig, S; Nicoll, D; Stradling, J R; Kohler, M

2009-03-01

Moderate-severe obstructive sleep apnoea (OSA) has been associated with several pro-atherogenic mechanisms and increased cardiovascular risk, but it is not known if minimally symptomatic OSA has similar effects. Circulating cell-derived microparticles have been shown to have pro-inflammatory, pro-coagulant and endothelial function-impairing effects, as well as to predict subclinical atherosclerosis and cardiovascular risk. In 57 patients with minimally symptomatic OSA, and 15 closely matched control subjects without OSA, AnnexinV-positive, platelet-, leukocyte- and endothelial cell-derived microparticles were measured by flow cytometry. In patients with OSA, median (interquartile range) levels of AnnexinV-positive microparticles were significantly elevated compared with control subjects: 2,586 (1,566-3,964) microL(-1) versus 1,206 (474-2,501) microL(-1), respectively. Levels of platelet-derived and leukocyte-derived microparticles were also significantly higher in patients with OSA (2,267 (1,102-3,592) microL(-1) and 20 (14-31) microL(-1), respectively) compared with control subjects (925 (328-2,068) microL(-1) and 15 (5-23) microL(-1), respectively). Endothelial cell-derived microparticle levels were similar in patients with OSA compared with control subjects (13 (8-25) microL(-1) versus 11 (6-17) microL(-1)). In patients with minimally symptomatic obstructive sleep apnoea, levels of AnnexinV-positive, platelet- and leukocyte-derived microparticles are elevated when compared with closely matched control subjects without obstructive sleep apnoea. These findings suggest that these patients may be at increased cardiovascular risk, despite being minimally symptomatic.

A Monolithic Electrochemical Micro Seismic Sensor Capable of Monitoring Three-Dimensional Vibrations

PubMed Central

Chen, Lianhong; Sun, Zhenyuan; Li, Guanglei; Chen, Deyong; Wang, Junbo

2018-01-01

A monolithic electrochemical micro seismic sensor capable of monitoring three-axial vibrations was proposed in this paper. The proposed micro sensor mainly consisted of four sensing units interconnected within flow channels and by interpreting the voltage outputs of the sensing units, vibrations with arbitrary directions can be quantified. The proposed seismic sensors are fabricated based on MEMS technologies and characterized, which produced sensitivities along x, y, and z axes as 2473.2 ± 184.5 V/(m/s), 2261.7 ± 119.6 V/(m/s), and 3480.7 ± 417.2 V/(m/s) at 30 Hz. In addition, the vibrations in x-y, x-z, and y-z planes were applied to the developed seismic sensors, leading to comparable monitoring results after decoupling calculations with the input velocities. Furthermore, the results have shown its feasibilities for seismic data recording. PMID:29614720

Collision count in rugby union: A comparison of micro-technology and video analysis methods.

PubMed

Reardon, Cillian; Tobin, Daniel P; Tierney, Peter; Delahunt, Eamonn

2017-10-01

The aim of our study was to determine if there is a role for manipulation of g force thresholds acquired via micro-technology for accurately detecting collisions in rugby union. In total, 36 players were recruited from an elite Guinness Pro12 rugby union team. Player movement profiles and collisions were acquired via individual global positioning system (GPS) micro-technology units. Players were assigned to a sub-category of positions in order to determine positional collision demands. The coding of collisions by micro-technology at g force thresholds between 2 and 5.5 g (0.5 g increments) was compared with collision coding by an expert video analyst using Bland-Altman assessments. The most appropriate g force threshold (smallest mean difference compared with video analyst coding) was lower for all forwards positions (2.5 g) than for all backs positions (3.5 g). The Bland-Altman 95% limits of agreement indicated that there may be a substantial over- or underestimation of collisions coded via GPS micro-technology when using expert video analyst coding as the reference comparator. The manipulation of the g force thresholds applied to data acquired by GPS micro-technology units based on incremental thresholds of 0.5 g does not provide a reliable tool for the accurate coding of collisions in rugby union. Future research should aim to investigate smaller g force threshold increments and determine the events that cause coding of false positives.

Unit: Micro-Organisms and Man, Inspection Pack, National Trial Print.

ERIC Educational Resources Information Center

Australian Science Education Project, Toorak, Victoria.

This unit, intended for students in grades eight or nine, is a revised version of ED 053 990. The teacher's guide lists the aims of the unit, behavioral objectives, suitable references and audio-visual aids, required apparatus and materials, and provides teaching notes for each activity, including comments concerning microbiological techniques.…

[Randomized, controlled clinical trials with observational follow-up investigations for evaluating efficacy of antihyperglycaemic treatment. I. Main results of the studies].

PubMed

Jermendy, György

2018-04-01

The effect of antihyperglycaemic (antidiabetic) treatment on the late diabetic complications is one of the most important research areas in clinical diabetology. The relationship between glycaemic control and late micro- and macrovascular complications was highlighted by the results of the DCCT (Diabetes Control and Complications Trial) with type 1 and by the UKPDS (United Kingdom Prospective Diabetes Study) with type 2 diabetic patients. In these studies, observational follow-up investigations were also performed after the close-out of the randomized phase of the trial. In addition to these landmark studies, other randomized, controlled efficacy trials were also performed with observational follow-up investigations resulting in the development of the concept of metabolic memory or metabolic legacy. In this article, the main results of the studies are summarized. Orv Hetil. 2018; 159(15): 575-582.

Dynamic Control System Performance during Commissioning of the Space Technology 7-Disturbance Reduction System Experiment of LISA Pathfinder

NASA Technical Reports Server (NTRS)

Hsu, Oscar; Maghami, Peiman; O’Donnell, James R., Jr.; Ziemer, John; Romero-Wolf, Andrew

2017-01-01

The Space Technology-7 Disturbance Reduction System (DRS) launched aboard the European Space Agency's LISA Pathfinder spacecraft on December 3, 2015, after more than a decade in development. DRS consists of three primary components: an Integrated Avionics Unit (IAU), Colloidal MicroNewton Thrusters, and Dynamic Control System (DCS) algorithms implemented on the IAU. During the portions of the mission in which the DRS was under control, the DCS was responsible for controlling the spacecraft and the free-floating test masses that were part of the LISA Test Package. The commissioning period was originally divided into two periods: before propulsion separation and after propulsion separation. A recommissioning period was added after an anomaly occurred in the thruster system. The paper will describe the activities used to commission DRS, present results from the commissioning of the DCS and the recommissioning activities per-formed after the thruster anomaly.

Dynamic Control System Performance during Commissioning of the Space Technology 7-Disturbance Reduction System Experiment of LISA Pathfinder

NASA Technical Reports Server (NTRS)

Hsu, Oscar; Maghami, Peiman; O’Donnell, James R., Jr.; Ziemer, John; Romero-Wolf, Andrew

2017-01-01

The Space Technology-7 Disturbance Reduction System (DRS) launched aboard the European Space Agencys LISA Pathfinder spacecraft on December 3, 2015, after more than a decade in development. DRS consists of three prima-ry components: an Integrated Avionics Unit (IAU), Colloidal MicroNewton Thrusters, and Dynamic Control System (DCS) algorithms implemented on the IAU. During the portions of the mission in which the DRS was under control, the DCS was responsible for controlling the spacecraft and the free-floating test masses that were part of the LISA Test Package. The commissioning period was originally divided into two periods: before propulsion separation and after pro-pulsion separation. A recommissioning period was added after an anomaly oc-curred in the thruster system. The paper will describe the activities used to com-mission DRS, present results from the commissioning of the DCS and the re-commissioning activities performed after the thruster anomaly.

Microelectromechanical Systems

NASA Technical Reports Server (NTRS)

Gabriel, Kaigham J.

1995-01-01

Micro-electromechanical systems (MEMS) is an enabling technology that merges computation and communication with sensing and actuation to change the way people and machines interact with the physical world. MEMS is a manufacturing technology that will impact widespread applications including: miniature inertial measurement measurement units for competent munitions and personal navigation; distributed unattended sensors; mass data storage devices; miniature analytical instruments; embedded pressure sensors; non-invasive biomedical sensors; fiber-optics components and networks; distributed aerodynamic control; and on-demand structural strength. The long term goal of ARPA's MEMS program is to merge information processing with sensing and actuation to realize new systems and strategies for both perceiving and controlling systems, processes, and the environment. The MEMS program has three major thrusts: advanced devices and processes, system design, and infrastructure.

Development of a Micro-UAV Hyperspectral Imaging Platform for Assessing Hydrogeological Hazards

NASA Astrophysics Data System (ADS)

Chen, Z.; Alabsi, M.

2015-12-01

The exacerbating global weather changes have cast significant impacts upon the proportion of water supplied to agriculture. Therefore, one of the 21stCentury Grant Challenges faced by global population is securing water for food. However, the soil-water behavior in an agricultural environment is complex; among others, one of the key properties we recognize is water repellence or hydrophobicity, which affects many hydrogeological and hazardous conditions such as excessive water infiltration, runoff, and soil erosion. Under a US-Israel research program funded by USDA and BARD at Israel, we have proposed the development of a novel micro-unmanned aerial vehicle (micro-UAV or drone) based hyperspectral imaging platform for identifying and assessing soil repellence at low altitudes with enhanced flexibility, much reduced cost, and ultimately easy use. This aerial imaging system consists of a generic micro-UAV, hyperspectral sensor aided by GPS/IMU, on-board computing units, and a ground station. The target benefits of this system include: (1) programmable waypoint navigation and robotic control for multi-view imaging; (2) ability of two- or three-dimensional scene reconstruction for complex terrains; and (3) fusion with other sensors to realize real-time diagnosis (e.g., humidity and solar irradiation that may affect soil-water sensing). In this talk we present our methodology and processes in integration of hyperspectral imaging, on-board sensing and computing, hyperspectral data modeling, and preliminary field demonstration and verification of the developed prototype.

Characterization of printable cellular micro-fluidic channels for tissue engineering.

PubMed

Zhang, Yahui; Yu, Yin; Chen, Howard; Ozbolat, Ibrahim T

2013-06-01

Tissue engineering has been a promising field of research, offering hope of bridging the gap between organ shortage and transplantation needs. However, building three-dimensional (3D) vascularized organs remains the main technological barrier to be overcome. One of the major challenges is the inclusion of a vascular network to support cell viability in terms of nutrients and oxygen perfusion. This paper introduces a new approach to the fabrication of vessel-like microfluidic channels that has the potential to be used in thick tissue or organ fabrication in the future. In this research, we investigate the manufacturability of printable micro-fluidic channels, where micro-fluidic channels support mechanical integrity as well as enable fluid transport in 3D. A pressure-assisted solid freeform fabrication platform is developed with a coaxial needle dispenser unit to print hollow hydrogel filaments. The dispensing rheology is studied, and effects of material properties on structural formation of hollow filaments are analyzed. Sample structures are printed through the developed computer-controlled system. In addition, cell viability and gene expression studies are presented in this paper. Cell viability shows that cartilage progenitor cells (CPCs) maintained their viability right after bioprinting and during prolonged in vitro culture. Real-time PCR analysis yielded a relatively higher expression of cartilage-specific genes in alginate hollow filament encapsulating CPCs, compared with monolayer cultured CPCs, which revealed that printable semi-permeable micro-fluidic channels provided an ideal environment for cell growth and function.

Characterization of Printable Cellular Micro-fluidic Channels for Tissue Engineering

PubMed Central

Zhang, Yahui; Yu, Yin; Chen, Howard; Ozbolat, Ibrahim T.

2014-01-01

Tissue engineering has been a promising field of research, offering hope of bridging the gap between organ shortage and transplantation needs. However, building three-dimensional (3D) vascularized organs remains the main technological barrier to be overcome. One of the major challenges is the inclusion of a vascular network to support cell viability in terms of nutrients and oxygen perfusion. This paper introduces a new approach to fabrication of vessel-like microfluidic channels that has the potential to be used in thick tissue or organ fabrication in the future. In this research, we investigate the manufacturability of printable micro-fluidic channels, where micro-fluidic channels support mechanical integrity as well as enable fluid transport in 3D. A pressure-assisted solid freeform fabrication platform is developed with a coaxial needle dispenser unit to print hollow hydrogel filaments. The dispensing rheology is studied, and effects of material properties on structural formation of hollow filaments are analyzed. Sample structures are printed through the developed computer-controlled system. In addition, cell viability and gene expression studies are presented in this paper. Cell viability shows that cartilage progenitor cells (CPCs) maintained their viability right after bioprinting and during prolonged in vitro culture. Real-time PCR analysis yielded relatively higher expression of cartilage-specific genes in alginate hollow filament encapsulating CPCs, compared with monolayer cultured CPCs, which revealed that printable semi-permeable micro-fluidic channels provided an ideal environment for cell growth and function. PMID:23458889

Effect of salinity and inorganic nitrogen concentrations on nitrification and denitrification rates in intertidal sediments and rocky biofilms of the Douro River estuary, Portugal.

PubMed

Magalhães, Catarina M; Joye, Samantha B; Moreira, Rosa M; Wiebe, William J; Bordalo, Adriano A

2005-05-01

The regulatory effects of salinity and inorganic nitrogen compounds on nitrification and denitrification were studied in intertidal sandy sediments and rocky biofilms in the Douro River estuary, Portugal, over a 12-month period. Nitrification and denitrification rates were measured in slurries of field samples and enrichment experiments using the difluoromethane and the acetylene inhibition techniques, respectively. Salinity did not regulate denitrification in either environment, suggesting that halotolerant bacteria dominated the denitrifier communities. However, nitrification rates were stimulated when salinity increased from 0 to 15 practical salinity units. NO3- addition experiments revealed that NO3- availability stimulates denitrification rates in sandy sediments, but not in rocky biofilms; however, in rocky biofilms a positive and linear relationship was observed between denitrification rates and water column NO3- concentrations (r=0.92) during the monthly surveys. The N2O:N2 ratios increased rapidly when NO3- increased from 63 to 363 microM; however, results from monthly surveys showed that environmental parameters other than NO3- availability may be important in controlling the variation in N2O production via denitrification. Ammonium additions to sandy sediments stimulated nitrification rates by 35% for the 20 microM NH4+ addition, but NH4+ appeared to inhibit nitrification at high concentration addition (200 microM NH4+). In contrast, rocky biofilm nitrification was stimulated by 65% when 200 microM NH4+ was added.

Biologically Inspired Micro-Flight Research

NASA Technical Reports Server (NTRS)

Raney, David L.; Waszak, Martin R.

2003-01-01

Natural fliers demonstrate a diverse array of flight capabilities, many of which are poorly understood. NASA has established a research project to explore and exploit flight technologies inspired by biological systems. One part of this project focuses on dynamic modeling and control of micro aerial vehicles that incorporate flexible wing structures inspired by natural fliers such as insects, hummingbirds and bats. With a vast number of potential civil and military applications, micro aerial vehicles represent an emerging sector of the aerospace market. This paper describes an ongoing research activity in which mechanization and control concepts for biologically inspired micro aerial vehicles are being explored. Research activities focusing on a flexible fixed- wing micro aerial vehicle design and a flapping-based micro aerial vehicle concept are presented.

A model for the Global Quantum Efficiency for a TPB-based wavelength-shifting system used with photomultiplier tubes in liquid argon in MicroBooNE

DOE PAGES

Pate, S. F.; Wester, T.; Bugel, L.; ...

2018-02-28

We present a model for the Global Quantum Efficiency (GQE) of the MicroBooNE optical units. An optical unit consists of a flat, circular acrylic plate, coated with tetraphenyl butadiene (TPB), positioned near the photocathode of a 20.2-cm diameter photomultiplier tube. The plate converts the ultra-violet scintillation photons from liquid argon into visible-spectrum photons to which the cryogenic phototubes are sensitive. The GQE is the convolution of the efficiency of the plates that convert the 128 nm scintillation light from liquid argon to visible light, the efficiency of the shifted light to reach the photocathode, and the efficiency of the cryogenic photomultiplier tube. We develop a GEANT4-based model of the optical unit, based on first principles, and obtain the range of probable values for the expected number of detected photoelectrons (more » $$N_{\\rm PE}$$) given the known systematic errors on the simulation parameters. We compare results from four measurements of the $$N_{\\rm PE}$$ determined using alpha-particle sources placed at two distances from a TPB-coated plate in a liquid argon cryostat test stand. We also directly measured the radial dependence of the quantum efficiency, and find that this has the same shape as predicted by our model. Our model results in a GQE of $$0.0055\\pm0.0009$$ for the MicroBooNE optical units. While the information shown here is MicroBooNE specific, the approach to the model and the collection of simulation parameters will be widely applicable to many liquid-argon-based light collection systems.« less

A model for the Global Quantum Efficiency for a TPB-based wavelength-shifting system used with photomultiplier tubes in liquid argon in MicroBooNE

DOE Office of Scientific and Technical Information (OSTI.GOV)

Pate, S. F.; Wester, T.; Bugel, L.

We present a model for the Global Quantum Efficiency (GQE) of the MicroBooNE optical units. An optical unit consists of a flat, circular acrylic plate, coated with tetraphenyl butadiene (TPB), positioned near the photocathode of a 20.2-cm diameter photomultiplier tube. The plate converts the ultra-violet scintillation photons from liquid argon into visible-spectrum photons to which the cryogenic phototubes are sensitive. The GQE is the convolution of the efficiency of the plates that convert the 128 nm scintillation light from liquid argon to visible light, the efficiency of the shifted light to reach the photocathode, and the efficiency of the cryogenic photomultiplier tube. We develop a GEANT4-based model of the optical unit, based on first principles, and obtain the range of probable values for the expected number of detected photoelectrons (more » $$N_{\\rm PE}$$) given the known systematic errors on the simulation parameters. We compare results from four measurements of the $$N_{\\rm PE}$$ determined using alpha-particle sources placed at two distances from a TPB-coated plate in a liquid argon cryostat test stand. We also directly measured the radial dependence of the quantum efficiency, and find that this has the same shape as predicted by our model. Our model results in a GQE of $$0.0055\\pm0.0009$$ for the MicroBooNE optical units. While the information shown here is MicroBooNE specific, the approach to the model and the collection of simulation parameters will be widely applicable to many liquid-argon-based light collection systems.« less

Spatial relationships between alcohol-related road crashes and retail alcohol availability.

PubMed

Morrison, Christopher; Ponicki, William R; Gruenewald, Paul J; Wiebe, Douglas J; Smith, Karen

2016-05-01

This study examines spatial relationships between alcohol outlet density and the incidence of alcohol-related crashes. The few prior studies conducted in this area used relatively large spatial units; here we use highly resolved units from Melbourne, Australia (Statistical Area level 1 [SA1] units: mean land area=0.5 km(2); SD=2.2 km(2)), in order to assess different micro-scale spatial relationships for on- and off-premise outlets. Bayesian conditional autoregressive Poisson models were used to assess cross-sectional relationships of three-year counts of alcohol-related crashes (2010-2012) attended by Ambulance Victoria paramedics to densities of bars, restaurants, and off-premise outlets controlling for other land use, demographic and roadway characteristics. Alcohol-related crashes were not related to bar density within local SA1 units, but were positively related to bar density in adjacent SA1 units. Alcohol-related crashes were negatively related to off-premise outlet density in local SA1 units. Examined in one metropolitan area using small spatial units, bar density is related to greater crash risk in surrounding areas. Observed negative relationships for off-premise outlets may be because the origins and destinations of alcohol-affected journeys are in distal locations relative to outlets. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

Functionalization of SBA-15 mesoporous silica by Cu-phosphonate units: Probing of synthesis route

DOE Office of Scientific and Technical Information (OSTI.GOV)

Laskowski, Lukasz, E-mail: lukasz.laskowski@kik.pcz.pl; Czestochowa University of Technology, Institute of Physics, Al. Armii Krajowej 19, 42-201 Czestochowa; Laskowska, Magdalena, E-mail: magdalena.laskowska@onet.pl

2014-12-15

Mesoporous silica SBA-15 containing propyl-copper phosphonate units was investigated. The structure of mesoporous samples was tested by N{sub 2} isothermal sorption (BET and BHJ analysis), TEM microscopy and X-Ray scattering. Quantitative analysis EDX has given information about proportions between component atoms in the sample. Quantitative elemental analysis has been carried out to support EDX. To examine bounding between copper atoms and phosphonic units the Raman spectroscopy was carried out. As a support of Raman scattering, the theoretical calculations were made based on density functional theory, with the B3LYP method. By comparison of the calculated vibrational spectra of the molecule withmore » experimental results, distribution of the active units inside silica matrix has been determined. - Graphical abstract: The present study is devoted to mesoporous silica SBA-15 containing propyl-copper phosphonate units. The species were investigated to confirm of synthesis procedure correctness by the micro-Raman technique combined with DFT numerical simulations. Complementary research was carried out to test the structure of mesoporous samples. - Highlights: • SBA-15 silica functionalized with propyl-copper phosphonate units was synthesized. • Synthesis efficiency probed by Raman study supported with DFT simulations. • Homogenous distribution of active units was proved. • Synthesis route enables precise control of distance between copper ions.« less

Spinal anaesthesia with a micro-catheter in high-risk patients undergoing colorectal cancer and other major abdominal surgery.

PubMed

Kumar, Chandra M; Corbett, William A; Wilson, Robert G

2008-08-01

Extended spinal anaesthesia using a spinal micro-catheter was used as a primary method of anaesthesia for elective colorectal cancer surgery in 68 high risk patients over a 14-year period in our institution. The technique was also useful in eight elective and 13 emergency abdominal surgeries. All patients suffered from severe chronic obstructive airway disease requiring multiple inhalers and drugs (ASA III). Thirty nine of these patients also suffered from angina, myocardial infarction, diabetes and other systemic diseases (ASA IV). Surgery included right hemicolectomy, left hemicolectomy, total colectomy, sigmoid colectomy, Hartman's resection, anterior resection of rectum, abdominoperineal resection, cholecystectomy (open and laparoscopic) and obstructed inguinal hernia requiring laparotomy. Spinal anaesthesia was performed under strict aseptic conditions with a 22 gauge spinal needle with a mixture consisting of 2.75ml of 0.5% heavy bupivacaine and 0.25ml of fentanyl (25microg). This was followed by placement of a spinal micro-catheter and the duration of anaesthesia was extended by intermittent injection of 0.5% isobaric bupivacaine. Brief hypotension occurred in 12.4% of patients during the establishment of anaesthetic block height to T6-7 and was duly treated with intravenous administration of fluid and ephedrine hydrochloride. Good anaesthesia resulted in all patients except for brief discomfort in some patients during hemicolectomy surgery possibly due to the dissection and traction on the peritoneum causing irritation to the diaphragm. The use of sedation was avoided. General anaesthesia was administered in one patient and this patient required postoperative ventilation and cardiovascular support in the Intensive Care Unit. The spinal micro-catheter was removed at the end of surgery. Postoperative pain relief was obtained by administering intravenous morphine through a patient controlled analgesia machine in the critical care ward area (High Dependency Unit). There was a low incidence of minor postoperative side effects such as nausea (14.6%), vomiting (7.9%), minor post dural puncture headache (5.6%) and pruritus (5.6%). We conclude that spinal anaesthesia with a micro-catheter may be used as a primary method of anaesthesia for colorectal cancer surgery and other major abdominal surgery in high-risk patients for whom general anaesthesia would be associated with higher morbidity and mortality.

Socioeconomic Status and Net Fertility during the Fertility Decline: A Comparative Analysis of Canada, Iceland, Sweden, Norway and the United States

PubMed Central

Dribe, Martin; Hacker, J. David; Scalone, Francesco

2014-01-01

Most previous work on the historical fertility transition has been macro-oriented, using aggregate data to examine economic correlates of demographic behaviour at regional or national levels, while much less has been done using micro data, and specifically looking at behavioural differentials among social groups. In this paper we study at the impact of socioeconomic status on net fertility during the fertility transition in five Northern American and European Countries (Canada, Iceland, Norway, Sweden and the USA). We use micro-level census data in 1900, containing information on number of children by age, occupation of the mother and father, place of residence and household context. The results show highly similar patterns across countries, with the elite and upper middle classes having considerably lower net fertility early in the transition. These patterns remain also after controlling for a range of individual and community-level fertility determinants and geographical unobserved heterogeneity. PMID:24684711

Design and fabrication of integrated micro/macrostructure for 3D functional gradient systems based on additive manufacturing

NASA Astrophysics Data System (ADS)

Yin, Ming; Xie, Luofeng; Jiang, Weifeng; Yin, Guofu

2018-05-01

Functional gradient systems have important applications in many areas. Although a 2D dielectric structure that serves as the gradient index medium for controlling electromagnetic waves is well established, it may not be suitable for application in 3D case. In this paper, we present a method to realize functional gradient systems with 3D integrated micro/macrostructure. The homogenization of the structure is studied in detail by conducting band diagram analysis. The analysis shows that the effective medium approximation is valid even when periodicity is comparable to wavelength. The condition to ensure the polarization-invariant, isotropic, and frequency-independent property is investigated. The scheme for the design and fabrication of 3D systems requiring spatial material property distribution is presented. By using the vat photopolymerization process, a large overall size of macrostructure at the system level and precise fine features of microstructure at the unit cell level are realized, thus demonstrating considerable scalability of the system for wave manipulation.

Three-dimensional macroporous nanoelectronic networks as minimally invasive brain probes

NASA Astrophysics Data System (ADS)

Xie, Chong; Liu, Jia; Fu, Tian-Ming; Dai, Xiaochuan; Zhou, Wei; Lieber, Charles M.

2015-12-01

Direct electrical recording and stimulation of neural activity using micro-fabricated silicon and metal micro-wire probes have contributed extensively to basic neuroscience and therapeutic applications; however, the dimensional and mechanical mismatch of these probes with the brain tissue limits their stability in chronic implants and decreases the neuron-device contact. Here, we demonstrate the realization of a three-dimensional macroporous nanoelectronic brain probe that combines ultra-flexibility and subcellular feature sizes to overcome these limitations. Built-in strains controlling the local geometry of the macroporous devices are designed to optimize the neuron/probe interface and to promote integration with the brain tissue while introducing minimal mechanical perturbation. The ultra-flexible probes were implanted frozen into rodent brains and used to record multiplexed local field potentials and single-unit action potentials from the somatosensory cortex. Significantly, histology analysis revealed filling-in of neural tissue through the macroporous network and attractive neuron-probe interactions, consistent with long-term biocompatibility of the device.

Issues regarding the usage of MPPT techniques in micro grid systems

NASA Astrophysics Data System (ADS)

Szeidert, I.; Filip, I.; Dragan, F.; Gal, A.

2018-01-01

The main objective of the control strategies applied at hybrid micro grid systems (wind/hydro/solar), that function based on maximum power point tracking (MPPT) techniques is to improve the conversion system’s efficiency and to preserve the quality of the generated electrical energy (voltage and power factor). One of the main goals of maximum power point tracking strategy is to achieve the harvesting of the maximal possible energy within a certain time period. In order to implement the control strategies for micro grid, there are typically required specific transducers (sensor for wind speed, optical rotational transducers, etc.). In the technical literature, several variants of the MPPT techniques are presented and particularized at some applications (wind energy conversion systems, solar systems, hydro plants, micro grid hybrid systems). The maximum power point tracking implementations are mainly based on two-level architecture. The lower level controls the main variable and the superior level represents the MPPT control structure. The paper presents micro grid structures developed at Politehnica University Timisoara (PUT) within the frame of a research grant. The paper is focused on the application of MPPT strategies on hybrid micro grid systems. There are presented several structures and control strategies and are highlighted their advantages and disadvantages, together with practical implementation guidelines.

Effect of curing mode on the micro-mechanical properties of dual-cured self-adhesive resin cements.

PubMed

Ilie, Nicoleta; Simon, Alexander

2012-04-01

Light supplying to luting resin cements is impeded in several clinical situations, causing us to question whether materials can properly be cured to achieve adequately (or adequate) mechanical properties. The aim of this study was therefore to analyse the effect of light on the micro-mechanical properties of eight popular dual-cured self-adhesive resin cements by comparing them with two conventional, also dual-cured, resin cements. Four different curing procedures were applied: auto-polymerisation (dark curing) and light curing (LED unit, Freelight 2, 20 s) by applying the unit directly on the samples' surface, at a distance of 5 and 10 mm. Twenty minutes after curing, the samples were stored for 1 week at 37°C in a water-saturated atmosphere. The micro-mechanical properties-Vickers hardness, modulus of elasticity, creep and elastic/plastic deformation-were measured. Data were analysed with multivariate ANOVA followed by Tukey's test and partial eta-squared statistics (p < 0.05). A very strong influence of the material as well as filler volume and weight on the micro-mechanical properties was measured, whereas the influence of the curing procedure and type of cement-conventional or self-adhesive-was generally low. The influence of light on the polymerisation process was material dependent, with four different behaviour patterns to be distinguished. As a material category, significantly higher micro-mechanical properties were measured for the conventional compared to the self-adhesive resin cements, although this difference was low. Within the self-adhesive resin cements group, the variation in micro-mechanical properties was high. The selection of suitable resin cements should be done by considering, besides its adhesive properties, its micro-mechanical properties and curing behaviour also.

Identification of optimal feedback control rules from micro-quadrotor and insect flight trajectories.

PubMed

Faruque, Imraan A; Muijres, Florian T; Macfarlane, Kenneth M; Kehlenbeck, Andrew; Humbert, J Sean

2018-06-01

This paper presents "optimal identification," a framework for using experimental data to identify the optimality conditions associated with the feedback control law implemented in the measurements. The technique compares closed loop trajectory measurements against a reduced order model of the open loop dynamics, and uses linear matrix inequalities to solve an inverse optimal control problem as a convex optimization that estimates the controller optimality conditions. In this study, the optimal identification technique is applied to two examples, that of a millimeter-scale micro-quadrotor with an engineered controller on board, and the example of a population of freely flying Drosophila hydei maneuvering about forward flight. The micro-quadrotor results show that the performance indices used to design an optimal flight control law for a micro-quadrotor may be recovered from the closed loop simulated flight trajectories, and the Drosophila results indicate that the combined effect of the insect longitudinal flight control sensing and feedback acts principally to regulate pitch rate.

A Cancer-Indicative microRNA Pattern in Normal Prostate Tissue

PubMed Central

Hellwinkel, Olaf J. C.; Sellier, Christina; Sylvester, Yu-Mi Jessica; Brase, Jan C.; Isbarn, Hendrik; Erbersdobler, Andreas; Steuber, Thomas; Sültmann, Holger; Schlomm, Thorsten; Wagner, Christina

2013-01-01

We analyzed the levels of selected micro-RNAs in normal prostate tissue to assess their potential to indicate tumor foci elsewhere in the prostate. Histologically normal prostate tissue samples from 31 prostate cancer patients and two cancer negative control groups with either unsuspicious or elevated prostate specific antigen (PSA) levels (14 and 17 individuals, respectively) were analyzed. Based on the expression analysis of 157 microRNAs in a pool of prostate tissue samples and information from data bases/literature, we selected eight microRNAs for quantification by real-time polymerase chain reactions (RT-PCRs). Selected miRNAs were analyzed in histologically tumor-free biopsy samples from patients and healthy controls. We identified seven microRNAs (miR-124a, miR-146a & b, miR-185, miR-16 and let-7a & b), which displayed significant differential expression in normal prostate tissue from men with prostate cancer compared to both cancer negative control groups. Four microRNAs (miR-185, miR-16 and let-7a and let-7b) remained to significantly discriminate normal tissues from prostate cancer patients from those of the cancer negative control group with elevated PSA levels. The transcript levels of these microRNAs were highly indicative for the presence of cancer in the prostates, independently of the PSA level. Our results suggest a microRNA-pattern in histologically normal prostate tissue, indicating prostate cancer elsewhere in the organ. PMID:23459235

The Surge of Micro-computers

NASA Astrophysics Data System (ADS)

Morita, Written By Ichiko; Matsumura, Translated By Tamiko

Libraries in the United States are now experiencing another phase of rapid change brought by the sudden invasion of micro-computers. Their versatility has made available not only capabilities for word processing and producing spread sheets but also introduced new approaches to large research libraries’ information networks. OCLC’s microenhancers are one example of what is now available. They can be used for retrospective conversion, provide instruction for the use of and function as front end terminals for large online catalogs. Various new ideas for the application of micro-computers will bring interesting developments in library automation.

Fabrication of flexible grating sensing waveguide based on nano-imprint lithography and micro-replication process

NASA Astrophysics Data System (ADS)

Liu, Yueming; Tian, Weijian; Zhang, Shaojun

2009-05-01

Soft and flexible grating sensing waveguides is urgently demanded in application of micro-bending sensing and surface distortion sensing in medical catheter and smart skin sensing unit etc. Based on Nano-imprint Lithography and micro-replication process, polymer grating waveguides with core size 4μm×20μm and pitch 0.75μm are fabricated successfully in this paper. This novel grating waveguides is soft and flexible enough for related application and with the bio-medical safe feature when used in human body catheter. Fabricated processes are presented including the fabrication of micro mould and UV-replication process, and relative skills are discussed also in this paper.

Sensor-less force-reflecting macro-micro telemanipulation systems by piezoelectric actuators.

PubMed

Amini, H; Farzaneh, B; Azimifar, F; Sarhan, A A D

2016-09-01

This paper establishes a novel control strategy for a nonlinear bilateral macro-micro teleoperation system with time delay. Besides position and velocity signals, force signals are additionally utilized in the control scheme. This modification significantly improves the poor transparency during contact with the environment. To eliminate external force measurement, a force estimation algorithm is proposed for the master and slave robots. The closed loop stability of the nonlinear micro-micro teleoperation system with the proposed control scheme is investigated employing the Lyapunov theory. Consequently, the experimental results verify the efficiency of the new control scheme in free motion and during collision between the slave robot and the environment of slave robot with environment, and the efficiency of the force estimation algorithm. Copyright © 2016 ISA. Published by Elsevier Ltd. All rights reserved.

MicroRNA Profile in Patients with Alzheimer's Disease: Analysis of miR-9-5p and miR-598 in Raw and Exosome Enriched Cerebrospinal Fluid Samples.

PubMed

Riancho, Javier; Vázquez-Higuera, José Luis; Pozueta, Ana; Lage, Carmen; Kazimierczak, Martha; Bravo, María; Calero, Miguel; Gonalezález, Andrea; Rodríguez, Eloy; Lleó, Alberto; Sánchez-Juan, Pascual

2017-01-01

MicroRNAs have been postulated as potential biomarkers for Alzheimer's disease (AD). Exosomes are nanovesicles which transport microRNAs, proteins, and other cargos. It has been hypothesized that the exosome traffic might be increased in neurodegenerative disorders. i) To assess the cerebrospinal fluid (CSF) microRNA profile in a group of AD patients and control subjects and to validate a group of microRNAs previously reported by other authors. ii) To compare microRNA levels in whole CSF and in the exosome-enriched fraction in AD patients. A panel of 760 microRNAs was analyzed in the CSF of 10 AD patients and 10 healthy subjects. Among microRNAs differently expressed, we selected those that had been previously reported by other authors. Candidates were validated in a larger group by individual qPCR assays. MicroRNA expression was also evaluated in exosome-enriched CSF samples of patients with AD and controls. Fifteen microRNAs were differently expressed in AD. MiR-9-5p, miR-134, and miR-598 were selected as candidates for further analysis. MiR-9-5p and miR-598 were detected in 50 and 75% of control CSF samples, respectively, while they were not detected in any AD CSF samples. We observed an opposite pattern when we evaluated the microRNA expression in the exosome-enriched CSF AD samples. No pattern variations were noted among healthy subjects. These data propose miR-9-5p and miR-598 as potential biomarkers for AD. Further studies in plasma and other body fluids will confirm their potential role as easily accessible biomarkers. In addition, our data suggest that exosome trafficking is different between AD and control subjects raising the need to take this phenomenon into consideration in future studies of AD biomarkers.

Method for evaluating compatibility of commercial electromagnetic (EM) microsensor tracking systems with surgical and imaging tables

NASA Astrophysics Data System (ADS)

Nafis, Christopher; Jensen, Vern; von Jako, Ron

2008-03-01

Electromagnetic (EM) tracking systems have been successfully used for Surgical Navigation in ENT, cranial, and spine applications for several years. Catheter sized micro EM sensors have also been used in tightly controlled cardiac mapping and pulmonary applications. EM systems have the benefit over optical navigation systems of not requiring a line-of-sight between devices. Ferrous metals or conductive materials that are transient within the EM working volume may impact tracking performance. Effective methods for detecting and reporting EM field distortions are generally well known. Distortion compensation can be achieved for objects that have a static spatial relationship to a tracking sensor. New commercially available micro EM tracking systems offer opportunities for expanded image-guided navigation procedures. It is important to know and understand how well these systems perform with different surgical tables and ancillary equipment. By their design and intended use, micro EM sensors will be located at the distal tip of tracked devices and therefore be in closer proximity to the tables. Our goal was to define a simple and portable process that could be used to estimate the EM tracker accuracy, and to vet a large number of popular general surgery and imaging tables that are used in the United States and abroad.

The Effect of Micro-ramps on Supersonic Flow over a Forward-Facing Step

NASA Astrophysics Data System (ADS)

Zhang, Qing-Hu; Yi, Shi-He; Zhu, Yang-Zhu; Chen, Zhi; Wu, Yu

2013-04-01

The effect of micro-ramp control on fully developed turbulent flow over a forward-facing step (FFS) is investigated in a supersonic low-noise wind tunnel at Mach number 3 using nano-tracer planar laser scattering (NPLS) and supersonic particle image velocimetry (PIV) techniques. High spatiotemporal resolution images and the average velocity profiles of supersonic flow over the FFS with and without the control of the micro-ramps are captured. The fine structures of both cases, including the coherent structures of fully developed boundary layer and the large-scale hairpin-like vortices originated from the micro-ramps as well as the interaction of shock waves with the large-scale structures, are revealed and compared. Based on the time-correlation images, the temporal and spatial evolutionary characteristics of the coherent structures are investigated. It is beneficial to understand the dynamic mechanisms of the separated flow and the control mechanisms of the micro-ramps. The size of the separation region is determined by the NPLS and PIV. The results indicate that the control of the micro-ramps is capable of delaying the separation and diminishing the extent of recirculation zone.

[Microbiological verification of a self control plan for a hospital food service].

PubMed

Torre, I; Pennino, F; Crispino, M

2006-01-01

During the past years, it has been an increment of food related infectious diseases. In order to avoid micro biological food contamination, adherence to good manufacturing is required through control measures of food safety practices. Updated national and European regulations underline the need to apply the HACCP system, overcoming the old concept of sample control on the end user product. This work shows results of microbiological controls made along the whole productive chain. Measurements are made using biomolecular techniques (PFGE) in order to assess the management of the micro biological risk of the self control plan applied to a hospital food service of Naples. The use of the PFGE applied on some micro-organisms gram negative potentially pathogen, underlines the circulation, continued in time, of these micro-organisms within the cooking area. In addition, cross contamination between several matrixes of samples has been detected.

Micro CT characterization of a coastal mine tailings deposit, Portmán Bay, SE Spain

NASA Astrophysics Data System (ADS)

Frigola, Jaime; Cerdà-Domènech, Marc; Barriuso, Eduardo; Sanchez-Vidal, Anna; Amblas, David; Canals, Miquel

2017-04-01

Scanning of sediment cores by means of high-resolution non-destructive techniques provides researchers with huge amounts of highly valuable data allowing fast and detailed characterization of the materials. In the last decades several devoted instruments have been developed and applied to the study of sedimentary sequences, mainly multi-sensor core loggers (MSCL) for the physical properties and XRF core scanners for the chemical elemental composition. The geoscientific community started using computed tomography (CT) systems about two decades ago. These were mainly medical systems as dedicated instruments were essentially lacking by that time. The resolution of those medical systems was limited to several hundreds of micrometres voxel size. Micro computed tomography (micro-CT) systems have also spread into geoscientific research, although their limited workspace dimensions prevents their use for large objects, such as long sediment cores. Recently, a new micro-CT system, the MultiTom Core X-ray CT, conceived by University of Barcelona (UB) researchers and developed by X-ray Engineering, became operational. It is able of scanning sediment cores up to 1.5 m long, and allows adjustable resolutions from 300 microns down to 3-4 microns. The system is now installed at UB's CORELAB Laboratory for non-destructive analyses of geological materials. Here we present, as an example, the results of MultiTom scans of a set of sediment cores recovered offshore Portmán Bay, SE Spain, in order to characterize at very high-resolution the metal-enriched deposit generated after 33 years of direct discharge into the sea of mine tailings resulting from the exploitation of Pb and Zn ores. In total 52 short cores and 6 long gravity cores from the mine tailings infilled bay were scanned with the MultiTom system at a mean voxel resolution of 125 microns. The integrated study of micro-CT data allowed differentiating the main tailings units from deposits formed after disposal cessation. Tailings units show higher radio-density values, which correspond to metal enrichments. A lower unit consists of highly laminated interbedded low radio-density and very high radio-density layers, while an upper mine tailings unit is more homogeneous and shows intermediate radio-density values. The limit between the tailings and the post-mining deposits is defined by a sharp surface associated with an abrupt decrease in the radio-densities. Post-mining deposits are also characterized by an increment in bioturbation marks, which are practically absent in the tailings units, and an increase in carbonate particles and organic matter patches. Micro CT scans allow observation of very small structures, which are indicative of the complexity of the sedimentation processes involved in the transport and final deposition of the mine tailings. Integration of micro CT scans together with XRF core scanner and MSCL data allows a better characterization of the metal concentrations and their distribution within the deposit, directly demonstrating the great value of non-destructive techniques for actually high-resolution sedimentological studies.

Advanced Micro Grid Energy Management Coupled with Integrated Volt/VAR Control for Improved Energy Efficiency, Energy Security, and Power Quality at DoD Installations

DTIC Science & Technology

2016-10-28

assumptions. List of Assumptions: Price of electrical energy : $0.07/kWh flat rate for energy at the base Price of peak power: $15/MW peak power...EW-201147) Advanced Micro-Grid Energy Management Coupled with Integrated Volt/VAR Control for Improved Energy Efficiency, Energy Security, and...12-C-0002 5b. GRANT NUMBER Advanced Micro-Grid Energy Management Coupled with Integrated Volt/VAR Control for Improved Energy Efficiency, Energy

Unbalanced voltage control of virtual synchronous generator in isolated micro-grid

NASA Astrophysics Data System (ADS)

Cao, Y. Z.; Wang, H. N.; Chen, B.

2017-06-01

Virtual synchronous generator (VSG) control is recommended to stabilize the voltage and frequency in isolated micro-grid. However, common VSG control is challenged by widely used unbalance loads, and the linked unbalance voltage problem worsens the power quality of the micro-grid. In this paper, the mathematical model of VSG was presented. Based on the analysis of positive- and negative-sequence equivalent circuit of VSG, an approach was proposed to eliminate the negative-sequence voltage of VSG with unbalance loads. Delay cancellation method and PI controller were utilized to identify and suppress the negative-sequence voltages. Simulation results verify the feasibility of proposed control strategy.

A wireless high-speed data acquisition system for geotechnical centrifuge model testing

NASA Astrophysics Data System (ADS)

Gaudin, C.; White, D. J.; Boylan, N.; Breen, J.; Brown, T.; DeCatania, S.; Hortin, P.

2009-09-01

This paper describes a novel high-speed wireless data acquisition system (WDAS) developed at the University of Western Australia for operation onboard a geotechnical centrifuge, in an enhanced gravitational field of up to 300 times Earth's gravity. The WDAS system consists of up to eight separate miniature units distributed around the circumference of a 0.8 m diameter drum centrifuge, communicating with the control room via wireless Ethernet. Each unit is capable of powering and monitoring eight instrument channels at a sampling rate of up to 1 MHz at 16-bit resolution. The data are stored within the logging unit in solid-state memory, but may also be streamed in real-time at low frequency (up to 10 Hz) to the centrifuge control room, via wireless transmission. The high-speed logging runs continuously within a circular memory (buffer), allowing for storage of a pre-trigger segment of data prior to an event. To suit typical geotechnical modelling applications, the system can record low-speed data continuously, until a burst of high-speed acquisition is triggered when an experimental event occurs, after which the system reverts back to low-speed acquisition to monitor the aftermath of the event. Unlike PC-based data acquisition solutions, this system performs the full sequence of amplification, conditioning, digitization and storage on a single circuit board via an independent micro-controller allocated to each pair of instrumented channels. This arrangement is efficient, compact and physically robust to suit the centrifuge environment. This paper details the design specification of the WDAS along with the software interface developed to control the units. Results from a centrifuge test of a submarine landslide are used to illustrate the performance of the new WDAS.

PROGRAMMABLE DISPLAY PUSHBUTTON LEGEND EDITOR

NASA Technical Reports Server (NTRS)

Busquets, A. M.

1994-01-01

The Programmable Display Pushbutton (PDP) is a pushbutton device available from Micro Switch which has a programmable 16 x 35 matrix of LEDs on the pushbutton surface. Any desired legends can be displayed on the PDPs, producing user-friendly applications which greatly reduce the need for dedicated manual controls. Because the PDP can interact with the operator, it can call for the correct response before transmitting its next message. It is both a simple manual control and a sophisticated programmable link between the operator and the host system. The Programmable Display Pushbutton Legend Editor, PDPE, is used to create the LED displays for the pushbuttons. PDPE encodes PDP control commands and legend data into message byte strings sent to a Logic Refresh and Control Unit (LRCU). The LRCU serves as the driver for a set of four PDPs. The legend editor (PDPE) transmits to the LRCU user specified commands that control what is displayed on the LED face of the individual pushbuttons. Upon receiving a command, the LRCU transmits an acknowledgement that the message was received and executed successfully. The user then observes the effect of the command on the PDP displays and decides whether or not to send the byte code of the message to a data file so that it may be called by an applications program. The PDPE program is written in FORTRAN for interactive execution. It was developed on a DEC VAX 11/780 under VMS. It has a central memory requirement of approximately 12800 bytes. It requires four Micro Switch PDPs and two RS-232 VAX 11/780 terminal ports. The PDPE program was developed in 1985.

Manufacturing and Characterization of Ti6Al4V Lattice Components Manufactured by Selective Laser Melting

PubMed Central

Campanelli, Sabina L.; Contuzzi, Nicola; Ludovico, Antonio D.; Caiazzo, Fabrizia; Cardaropoli, Francesco; Sergi, Vincenzo

2014-01-01

The paper investigates the fabrication of Selective Laser Melting (SLM) titanium alloy Ti6Al4V micro-lattice structures for the production of lightweight components. Specifically, the pillar textile unit cell is used as base lattice structure and alternative lattice topologies including reinforcing vertical bars are also considered. Detailed characterizations of dimensional accuracy, surface roughness, and micro-hardness are performed. In addition, compression tests are carried out in order to evaluate the mechanical strength and the energy absorbed per unit mass of the lattice truss specimens made by SLM. The built structures have a relative density ranging between 0.2234 and 0.5822. An optimization procedure is implemented via the method of Taguchi to identify the optimal geometric configuration which maximizes peak strength and energy absorbed per unit mass. PMID:28788707

Correlating bilayer tablet delamination tendencies to micro-environmental thermodynamic conditions during pan coating.

PubMed

Zacour, Brian M; Pandey, Preetanshu; Subramanian, Ganeshkumar; Gao, Julia Z; Nikfar, Faranak

2014-06-01

The objective of this study was to determine the impact that the micro-environment, as measured by PyroButton data loggers, experienced by tablets during the pan coating unit operation had on the layer adhesion of bilayer tablets in open storage conditions. A full factorial design of experiments (DOE) with three center points was conducted to study the impact of final tablet hardness, film coating spray rate and film coating exhaust temperature on the delamination tendencies of bilayer tablets. PyroButton data loggers were placed (fixed) at various locations in a pan coater and were also allowed to freely move with the tablet bed to measure the micro-environmental temperature and humidity conditions of the tablet bed. The variance in the measured micro-environment via PyroButton data loggers accounted for 75% of the variance in the delamination tendencies of bilayer tablets on storage (R(2 )= 0.75). A survival analysis suggested that tablet hardness and coating spray rate significantly impacted the delamination tendencies of the bilayer tablets under open storage conditions. The coating exhaust temperature did not show good correlation with the tablets' propensity to crack indicating that it was not representative of the coating micro-environment. Models created using data obtained from the PyroButton data loggers outperformed models created using primary DOE factors in the prediction of bilayer tablet strength, especially upon equipment or scale transfers. The coating micro-environment experienced by tablets during the pan coating unit operation significantly impacts the strength of the bilayer interface of tablets on storage.

MEMS based pumped liquid cooling systems for micro/nano spacecraft thermal control

NASA Technical Reports Server (NTRS)

Birur, G. C.; Shakkottai, P.; Sur, T. W.

2000-01-01

The electronic and other payload power densities in future micro/nano spacecraft are expected to exceed 25 Watts/cm(sup 2) and require advanced thermal control concepts and technologies to keep their payload within allowable temperature limits. This paper presents background on the need for pumped liquid cooling systems for future micro/nano spacecraft and results from this ongoing experimental investigation.

Fully-automated, high-throughput micro-computed tomography analysis of body composition enables therapeutic efficacy monitoring in preclinical models.

PubMed

Wyatt, S K; Barck, K H; Kates, L; Zavala-Solorio, J; Ross, J; Kolumam, G; Sonoda, J; Carano, R A D

2015-11-01

The ability to non-invasively measure body composition in mouse models of obesity and obesity-related disorders is essential for elucidating mechanisms of metabolic regulation and monitoring the effects of novel treatments. These studies aimed to develop a fully automated, high-throughput micro-computed tomography (micro-CT)-based image analysis technique for longitudinal quantitation of adipose, non-adipose and lean tissue as well as bone and demonstrate utility for assessing the effects of two distinct treatments. An initial validation study was performed in diet-induced obesity (DIO) and control mice on a vivaCT 75 micro-CT system. Subsequently, four groups of DIO mice were imaged pre- and post-treatment with an experimental agonistic antibody specific for anti-fibroblast growth factor receptor 1 (anti-FGFR1, R1MAb1), control immunoglobulin G antibody, a known anorectic antiobesity drug (rimonabant, SR141716), or solvent control. The body composition analysis technique was then ported to a faster micro-CT system (CT120) to markedly increase throughput as well as to evaluate the use of micro-CT image intensity for hepatic lipid content in DIO and control mice. Ex vivo chemical analysis and colorimetric analysis of the liver triglycerides were performed as the standard metrics for correlation with body composition and hepatic lipid status, respectively. Micro-CT-based body composition measures correlate with ex vivo chemical analysis metrics and enable distinction between DIO and control mice. R1MAb1 and rimonabant have differing effects on body composition as assessed by micro-CT. High-throughput body composition imaging is possible using a modified CT120 system. Micro-CT also provides a non-invasive assessment of hepatic lipid content. This work describes, validates and demonstrates utility of a fully automated image analysis technique to quantify in vivo micro-CT-derived measures of adipose, non-adipose and lean tissue, as well as bone. These body composition metrics highly correlate with standard ex vivo chemical analysis and enable longitudinal evaluation of body composition and therapeutic efficacy monitoring.

Assessing consumption of bioactive micro-particles by filter-feeding Asian carp

USGS Publications Warehouse

Jensen, Nathan R.; Amberg, Jon J.; Luoma, James A.; Walleser, Liza R.; Gaikowski, Mark P.

2012-01-01

Silver carp Hypophthalmichthys molitrix (SVC) and bighead carp H. nobilis (BHC) have impacted waters in the US since their escape. Current chemical controls for aquatic nuisance species are non-selective. Development of a bioactive micro-particle that exploits filter-feeding habits of SVC or BHC could result in a new control tool. It is not fully understood if SVC or BHC will consume bioactive micro-particles. Two discrete trials were performed to: 1) evaluate if SVC and BHC consume the candidate micro-particle formulation; 2) determine what size they consume; 3) establish methods to evaluate consumption of filter-feeders for future experiments. Both SVC and BHC were exposed to small (50-100 μm) and large (150-200 μm) micro-particles in two 24-h trials. Particles in water were counted electronically and manually (microscopy). Particles on gill rakers were counted manually and intestinal tracts inspected for the presence of micro-particles. In Trial 1, both manual and electronic count data confirmed reductions of both size particles; SVC appeared to remove more small particles than large; more BHC consumed particles; SVC had fewer overall particles in their gill rakers than BHC. In Trial 2, electronic counts confirmed reductions of both size particles; both SVC and BHC consumed particles, yet more SVC consumed micro-particles compared to BHC. Of the fish that ate micro-particles, SVC consumed more than BHC. It is recommended to use multiple metrics to assess consumption of candidate micro-particles by filter-feeders when attempting to distinguish differential particle consumption. This study has implications for developing micro-particles for species-specific delivery of bioactive controls to help fisheries, provides some methods for further experiments with bioactive micro-particles, and may also have applications in aquaculture.

Recent Results from the MicroMAS Global Environmental MonitoringNanosatellite Mission

NASA Astrophysics Data System (ADS)

Blackwell, W. J.; Cahoy, K.

2014-12-01

The Micro-sized Microwave Atmospheric Satellite (MicroMAS) is a dual-spinning 3U CubeSat equipped with apassive microwave radiometer that observes in nine channels near the 118.75-GHz oxygen absorption line.MicroMAS is designed to observe convective thunderstorms, tropical cyclones, and hurricanes from a midinclinationorbit. The MicroMAS flight unit was developed by MIT Lincoln Laboratory and the MIT Space SystemsLaboratory and was launched to the International Space Station on July 13, 2014, and scheduled for an earlySeptember deployment for a ~90-day mission. The payload is housed in the "lower" 1U of the dual-spinning 3UCubeSat and mechanically rotated approximately once per second as the spacecraft orbits the Earth, resulting in across-track scanned beam with a full-width half-max (FWHM) beamwidth of 2.4 degrees and an approximately 17-km diameter footprint at nadir incidence from a nominal altitude of 400 km. The relatively low cost of MicroMASenables the deployment of a constellation of sensors, spaced equally around several orbit planes. A small fleet ofMicroMAS systems could yield high-resolution global temperature and water vapor profiles, as well as cloudmicrophysical and precipitation parameters.Significant advancements were made in the Assembly, Integration, and Test phase of the project developmentlifecycle. The flight software and communications architecture was refined and tested in relevant lab facilities. Thepower subsystem was modified to include additional required inhibits for the ISS launch. Hardware in the loop testsas well as simulations of the attitude determination and control system (ADCS) were performed to validate theunique dual-spinning, local vertical, local horizontal (LVLH) stabilized flight design. ADCS algorithms were testedon a 3-axis air bearing and custom rig inside a 3-axis programmable Helmholtz cage. Finally, the integratedspacecraft underwent a series of environmental tests in order to verify the results of thermal modeling analyses,prove the performance of critical design components in relevant environmental conditions, and validate the softwareand concept-of-operations developed for flight. We present these advancements, lessons-learned in developing ascience-oriented CubeSat system, and any available launch/on-orbit updates.

Paediatric dose reduction with the introduction of digital fluorography.

PubMed

Mooney, R B; McKinstry, J

2001-01-01

Fluoroscopy guided examinations in a paediatric X ray department were initially carried out on a unit that used a conventional screen-film combination for spot-films. A new fluoroscopy unit was installed with the facilities of digital fluorography and last image hold. Comparison of equipment performance showed that the dose per image for screen-film and digital fluorography was 3 microGy and 0.4 microGy, respectively. Although the screen-film had superior image quality, the department's radiologist confirmed that digital fluorography provided a diagnostic image. Patient dose measurements showed that introduction of the new unit caused doses to fall by an average of 70%, although fluoroscopy time had not changed significantly. The new unit produced 40% less air kerma during fluoroscopy. The remaining 30% reduction in dose was due to the introduction of digital fluorography and last image hold facilities. It is concluded that the use of digital fluorography can be an effective way of reducing paediatric dose.

High quality-factor quartz tuning fork glass probe used in tapping mode atomic force microscopy for surface profile measurement

NASA Astrophysics Data System (ADS)

Chen, Yuan-Liu; Xu, Yanhao; Shimizu, Yuki; Matsukuma, Hiraku; Gao, Wei

2018-06-01

This paper presents a high quality-factor (Q-factor) quartz tuning fork (QTF) with a glass probe attached, used in frequency modulation tapping mode atomic force microscopy (AFM) for the surface profile metrology of micro and nanostructures. Unlike conventionally used QTFs, which have tungsten or platinum probes for tapping mode AFM, and suffer from a low Q-factor influenced by the relatively large mass of the probe, the glass probe, which has a lower density, increases the Q-factor of the QTF probe unit allowing it to obtain better measurement sensitivity. In addition, the process of attaching the probe to the QTF with epoxy resin, which is necessary for tapping mode AFM, is also optimized to further improve the Q-factor of the QTF glass probe. The Q-factor of the optimized QTF glass probe unit is demonstrated to be very close to that of a bare QTF without a probe attached. To verify the effectiveness and the advantages of the optimized QTF glass probe unit, the probe unit is integrated into a home-built tapping mode AFM for conducting surface profile measurements of micro and nanostructures. A blazed grating with fine tool marks of 100 nm, a microprism sheet with a vertical amplitude of 25 µm and a Fresnel lens with a steep slope of 90 degrees are used as measurement specimens. From the measurement results, it is demonstrated that the optimized QTF glass probe unit can achieve higher sensitivity as well as better stability than conventional probes in the measurement of micro and nanostructures.

Dysregulation of serum microRNA-574-3p and its clinical significance in hepatocellular carcinoma.

PubMed

Shen, Xianjuan; Xue, Yajing; Cong, Hui; Wang, Xudong; Ju, Shaoqing

2018-07-01

Objectives To explore microRNA-574-3p expression in serum of patients with hepatocellular carcinoma and investigate correlations between serum microRNA-574-3p expression and the development and prognosis of hepatocellular carcinoma. Design and methods Serum samples were collected from 70 patients with primary hepatocellular carcinoma, 40 patients with cirrhosis and 45 healthy controls. Serum microRNA-574-3p expression levels were detected by real-time quantitative polymerase chain reaction. The linearity, specificity and reproducibility were evaluated. In addition, the diagnostic value of microRNA-574-3p and its correlations with clinicopathologic features were assessed. Results The relative expression of microRNA-574-3p in hepatocellular carcinoma patients, cirrhosis patients and healthy controls was 2.306 (1.801-3.130), 1.362 (0.994-1.665) and 1.263 (0.765-1.723), respectively, indicating that it was significantly higher in hepatocellular carcinoma patients than that in the other two groups ( U = 439.5, 514.5, both P < 0.0001) and was significantly correlated with hepatitis B virus DNA copies ( U = 383.0, P = 0.018). In hepatitis B virus-positive hepatocellular carcinoma patients, the relative expression of microRNA-574-3p was significantly correlated with hepatitis B virus DNA concentration ( r = 0.348, P = 0.022). Compared with healthy control group, AUC ROC of serum microRNA-574-3p in hepatocellular carcinoma group was 0.837 with 95% CI: 0.763-0.910. Combining microRNA-574-3p, AFU and alpha-fetoprotein together, the sensitivity was highest compared with other markers alone or combined. Conclusions The relative expression of serum microRNA-574-3p in hepatocellular carcinoma patients was significantly higher than that in cirrhosis patients and healthy controls, and it may be an important biomarker in the auxiliary diagnosis of hepatocellular carcinoma.

Photothermal generation of microbubbles on plasmonic nanostructures inside microfluidic channels

NASA Astrophysics Data System (ADS)

Li, Jingting; Li, Ming; Santos, Greggy M.; Zhao, Fusheng; Shih, Wei-Chuan

2016-03-01

Microbubbles have been utilized as micro-pumps, micro-mixers, micro-valves, micro-robots and surface cleaners. Various generation techniques can be found in the literature, including resistive heating, hydrodynamic methods, illuminating patterned metal films and noble metal nanoparticles of Au or Ag. We present photothermal microbubble generation by irradiating nanoporous gold disk covered microfluidic channels. The size of the microbubble can be controlled by adjusting the laser power. The dynamics of both bubble growth and shrinkage are studied. The advantages of this technique are flexible bubble generation locations, long bubble lifetimes, no need for light-adsorbing dyes, high controllability over bubble size, low power consumption, etc. This technique has the potential to provide new flow control functions in microfluidic devices.

Micro-controller based air pressure monitoring instrumentation system using optical fibers as sensor

NASA Astrophysics Data System (ADS)

Hazarika, D.; Pegu, D. S.

2013-03-01

This paper describes a micro-controller based instrumentation system to monitor air pressure using optical fiber sensors. The principle of macrobending is used to develop the sensor system. The instrumentation system consists of a laser source, a beam splitter, two multi mode optical fibers, two Light Dependent Resistance (LDR) based timer circuits and a AT89S8252 micro-controller. The beam splitter is used to divide the laser beam into two parts and then these two beams are launched into two multi mode fibers. One of the multi mode fibers is used as the sensor fiber and the other one is used as the reference fiber. The use of the reference fiber is to eliminate the environmental effects while measuring the air pressure magnitude. The laser beams from the sensor and reference fibers are applied to two identical LDR based timer circuits. The LDR based timer circuits are interfaced to a micro-controller through its counter pins. The micro-controller samples the frequencies of the timer circuits using its counter-0 and counter-1 and the counter values are then processed to provide the measure of air pressure magnitude.

Preliminary design of mesoscale turbocompressor and rotordynamics tests of rotor bearing system

NASA Astrophysics Data System (ADS)

Hossain, Md Saddam

2011-12-01

A mesoscale turbocompressor spinning above 500,000 RPM is evolutionary technology for micro turbochargers, turbo blowers, turbo compressors, micro-gas turbines, auxiliary power units, etc for automotive, aerospace, and fuel cell industries. Objectives of this work are: (1) to evaluate different air foil bearings designed for the intended applications, and (2) to design & perform CFD analysis of a micro-compressor. CFD analysis of shrouded 3-D micro compressor was conducted using Ansys Bladegen as blade generation tool, ICEM CFD as mesh generation tool, and CFX as main solver for different design and off design cases and also for different number of blades. Comprehensive experimental facilities for testing the turbocompressor system have been also designed and proposed for future work.

Soft Real-Time PID Control on a VME Computer

NASA Technical Reports Server (NTRS)

Karayan, Vahag; Sander, Stanley; Cageao, Richard

2007-01-01

microPID (uPID) is a computer program for real-time proportional + integral + derivative (PID) control of a translation stage in a Fourier-transform ultraviolet spectrometer. microPID implements a PID control loop over a position profile at sampling rate of 8 kHz (sampling period 125microseconds). The software runs in a strippeddown Linux operating system on a VersaModule Eurocard (VME) computer operating in real-time priority queue using an embedded controller, a 16-bit digital-to-analog converter (D/A) board, and a laser-positioning board (LPB). microPID consists of three main parts: (1) VME device-driver routines, (2) software that administers a custom protocol for serial communication with a control computer, and (3) a loop section that obtains the current position from an LPB-driver routine, calculates the ideal position from the profile, and calculates a new voltage command by use of an embedded PID routine all within each sampling period. The voltage command is sent to the D/A board to control the stage. microPID uses special kernel headers to obtain microsecond timing resolution. Inasmuch as microPID implements a single-threaded process and all other processes are disabled, the Linux operating system acts as a soft real-time system.

A Multiagent Energy Management System for a Small Microgrid Equipped with Power Sources and Energy Storage Units

NASA Astrophysics Data System (ADS)

Radziszewska, Weronika; Nahorski, Zbigniew

An Energy Management System (EMS) for a small microgrid is presented, with both demand and production side management. The microgrid is equipped with renewable and controllable power sources (like a micro gas turbine), energy storage units (batteries and flywheels). Energy load is partially scheduled to avoid extreme peaks of power demand and to possibly match forecasted energy supply from the renewable power sources. To balance the energy in the network on line, a multiagent system is used. Intelligent agents of each device are proactively acting towards balancing the energy in the network, and at the same time optimizing the cost of operation of the whole system. A semi-market mechanism is used to match a demand and a production of the energy. Simulations show that the time of reaching a balanced state does not exceed 1 s, which is fast enough to let execute proper balancing actions, e.g. change an operating point of a controllable energy source. Simulators of sources and consumption devices were implemented in order to carry out exhaustive tests.

[Research of preparation craft of Danshen phenolic acid fast release unit in multi-drug delivery system of Tongmai micro-pellets].

PubMed

Chen, Bin; Xiao, Wei; Jia, Xiao-Bin; Huang, Yang

2012-07-01

To prepare Danshen phenolic acid fast release micro-pellets and study its preparation craft. The factors which could impact yield, extrude shaping, dissolution of Danshen phenolic acid micro-pellets such as wetting agent, drug loading dose, adjuvant, lactose dose, disintegrant, CMS-Na dose and wetting agent dose was investigated. The optimum preparation craft of Danshen phenolic acid fast release micro-pellets was screened out by orhogonal design. Formula of Danshen phenolic acid fast release micro-pellets was calculated as volume dose 50 g. The formula was as follows: principal agent 22.5 g, lactose 5 g, CMS-Na 2 g, MCC 20.5 g, 27 mL 30% ethanol as wetting agent. Extrusion-spheronization was applied. The optimum conditions were screened out as follows: extrusion frequency (25 Hz), spheronization machine frequency (50 Hz), spheronization time (4 min). The process was scientific and rational. The preparation is stable settles basis for multi-drug delivery system of Tongmai micro-pellets.

A force-controllable macro-micro manipulator and its application to medical robots

NASA Technical Reports Server (NTRS)

Marzwell, Neville I.; Uecker, Darrin R.; Wang, Yulun

1994-01-01

This paper describes an 8-degrees-of-freedom macro-micro robot. This robot is capable of performing tasks that require accurate force control, such as polishing, finishing, grinding, deburring, and cleaning. The design of the macro-micro mechanism, the control algorithms, and the hardware/software implementation of the algorithms are described in this paper. Initial experimental results are reported. In addition, this paper includes a discussion of medical surgery and the role that force control may play. We introduce a new class of robotic systems collectively called Robotic Enhancement Technology (RET). RET systems introduce the combination of robotic manipulation with human control to perform manipulation tasks beyond the individual capability of either human or machine. The RET class of robotic systems offers new challenges in mechanism design, control-law development, and man/machine interface design. We believe force-controllable mechanisms such as the macro-micro structure we have developed are a necessary part of RET. Work in progress in the area of RET systems and their application to minimally invasive surgery is presented, along with future research directions.

Carbon Nanotube Bolometer for Absolute FTIR Spectroscopy

NASA Astrophysics Data System (ADS)

Woods, Solomon; Neira, Jorge; Tomlin, Nathan; Lehman, John

We have developed and calibrated planar electrical-substitution bolometers which employ absorbers made from vertically-aligned carbon nanotube arrays. The nearly complete absorption of light by the carbon nanotubes from the visible range to the far-infrared can be exploited to enable a device with read-out in native units equivalent to optical power. Operated at cryogenic temperatures near 4 K, these infrared detectors are designed to have time constant near 10 ms and a noise floor of about 10 pW. Built upon a micro-machined silicon platform, each device has an integrated heater and thermometer, either a carbon nanotube thermistor or superconducting transition edge sensor, for temperature control. We are optimizing temperature-controlled measurement techniques to enable high resolution spectral calibrations using these devices with a Fourier-transform spectrometer.

Changes in symbiotic and associative interrelations in a higher plant-bacterial system during space flight

NASA Astrophysics Data System (ADS)

Kordyum, V. A.; Man'ko, V. G.; Popova, A. F.; Shcherbak, O. H.; Mashinsky, A. L.; Nguen-Hgue-Thyok

The miniature cenosis consisting of the water fern Azolla with its associated symbiotic nitrogen-fixing cyanobacterium Anabaena and the concomitant bacteria was investigated. Ecological closure was shown to produce sharp quantitative and qualitative changes in the number and type of concomitant bacteria. Changes in the distribution of bacterial types grown on beef-extract broth after space flight were recorded. Anabaena azollae underwent the most significant changes under spaceflight conditions. Its cell number per Azolla biomass unit increased substantially. Thus closure of cenosis resulted in a weakening of control over microbial development by Azolla. This tendency was augmented by spaceflight factors. Reduction in control exerted by macro-organisms over development of associated micro-organisms must be taken into account in constructing closed ecological systems in the state of weightlessness.

Power-gated 32 bit microprocessor with a power controller circuit activated by deep-sleep-mode instruction achieving ultra-low power operation

NASA Astrophysics Data System (ADS)

Koike, Hiroki; Ohsawa, Takashi; Miura, Sadahiko; Honjo, Hiroaki; Ikeda, Shoji; Hanyu, Takahiro; Ohno, Hideo; Endoh, Tetsuo

2015-04-01

A spintronic-based power-gated micro-processing unit (MPU) is proposed. It includes a power control circuit activated by the newly supported power-off instruction for the deep-sleep mode. These means enable the power-off procedure for the MPU to be executed appropriately. A test chip was designed and fabricated using 90 nm CMOS and an additional 100 nm MTJ process; it was successfully operated. The guideline of the energy reduction effects for this MPU was presented, using the estimation based on the measurement results of the test chip. The result shows that a large operation energy reduction of 1/28 can be achieved when the operation duty is 10%, under the condition of a sufficient number of idle clock cycles.

MEMS device for spacecraft thermal control applications

NASA Technical Reports Server (NTRS)

Swanson, Theordore D. (Inventor)

2003-01-01

A micro-electromechanical device that comprises miniaturized mechanical louvers, referred to as Micro Electro-Mechanical Systems (MEMS) louvers are employed to achieve a thermal control function for spacecraft and instruments. The MEMS louvers are another form of a variable emittance control coating and employ micro-electromechanical technology. In a function similar to traditional, macroscopic thermal louvers, the MEMS louvers of the present invention change the emissivity of a surface. With the MEMS louvers, as with the traditional macroscopic louvers, a mechanical vane or window is opened and closed to allow an alterable radiative view to space.

Dynamics, Control, and Fabrication of Micro Embedded Heaters and Sensors for Micro SMA Active Endoscopes

NASA Astrophysics Data System (ADS)

Aphanuphong, Sutha

This research investigates design and control of an active catheter for minimally invasive medical procedures. Microfabrication techniques are developed and several prototypes were constructed. The understanding and analysis results from each design iteration are utilized to improve the overall design and the performance of each revision. An innovative co-fabrication method is explored to simplify the fabrication process and also improve the quality, repeatability, and reliability of the active catheter. This co-fabrication method enables a unique compact integrated heater and sensor film to be directly constructed on a shape memory alloy (SMA) sheet and to be utilized as an outline mask to pattern a micro SMA actuator. There are two functions integrated in the sensor film: heat sources to actuate the micro SMA actuator and sensors to provide temperature and strain of the active catheter to closed-loop control algorithms. Three main aspects are explored in this dissertation: thermal dynamics in the MicroFlex (muF) film and its effect on the sensor capabilities; non-minimum phase behavior and its effect on control performance, and film micro fabrication design and its effect on thermal dynamics. The sensor film developed from this understanding is able to deliver excellent heating and sensing performance with a simple design.

Contamination Study of Micro Pulsed Plasma Thruster

DTIC Science & Technology

2008-03-01

missions ranging from nano -satellites to large spacecrafts requiring precision placement1. As an unfortunate side effect, the exhaust plume induces...the electrodes9. The electrodes have often been arranged coaxially . The system consists of electrodes, capacitors as energy storage units, a spark...increasing interest in the so-called micro- and nano -satellites, which are highly maneuverable and have lower cost. These small satellites are aimed to

Large depth of focus dynamic micro integral imaging for optical see-through augmented reality display using a focus-tunable lens.

PubMed

Shen, Xin; Javidi, Bahram

2018-03-01

We have developed a three-dimensional (3D) dynamic integral-imaging (InIm)-system-based optical see-through augmented reality display with enhanced depth range of a 3D augmented image. A focus-tunable lens is adopted in the 3D display unit to relay the elemental images with various positions to the micro lens array. Based on resolution priority integral imaging, multiple lenslet image planes are generated to enhance the depth range of the 3D image. The depth range is further increased by utilizing both the real and virtual 3D imaging fields. The 3D reconstructed image and the real-world scene are overlaid using an optical see-through display for augmented reality. The proposed system can significantly enhance the depth range of a 3D reconstructed image with high image quality in the micro InIm unit. This approach provides enhanced functionality for augmented information and adjusts the vergence-accommodation conflict of a traditional augmented reality display.

Breakthrough: micro-electronic photovoltaics

ScienceCinema

Okandan, Murat; Gupta, Vipin

2018-01-16

Sandia developed tiny glitter-sized photovoltaic (PV) cells that could revolutionize solar energy collection. The crystalline silicon micro-PV cells will be cheaper and have greater efficiencies than current PV collectors. Micro-PV cells require relatively little material to form well-controlled, highly efficient devices. Cell fabrication uses common microelectric and micro-electromechanical systems (MEMS) techniques.

Understanding Micro-Ramp Control for Shock Boundary Layer Interactions

DTIC Science & Technology

2008-02-07

micro-ramps on a supersonic boundary layer at M=3.0 was investigated using monotone integrated Large Eddy Simulations (MILES) and Reynolds Averaged Navier... Supersonic boundary layer flow with micro-ramp and no shock wave 3.2 SBLI with no micro-ramp 3.3 SBLI with micro-ramp 3.4 Micro-ramp size and location IV . C...ramps on a supersonic boundary layer at M=3.0 was investigated using monotone integrated Large Eddy Simulations (MILES) and Reynolds Averaged Navier

Integrated Micro/nanoengineered Functional Biomaterials for Cell Mechanics and Mechanobiology: A Materials Perspective

PubMed Central

Shao, Yue

2014-01-01

The rapid development of micro/nanoengineered functional biomaterials in the last two decades has empowered materials scientists and bioengineers to precisely control different aspects of the in vitro cell microenvironment. Following a philosophy of reductionism, many studies using synthetic functional biomaterials have revealed instructive roles of individual extracellular biophysical and biochemical cues in regulating cellular behaviors. Development of integrated micro/nanoengineered functional biomaterials to study complex and emergent biological phenomena has also thrived rapidly in recent years, revealing adaptive and integrated cellular behaviors closely relevant to human physiological and pathological conditions. Working at the interface between materials science and engineering, biology, and medicine, we are now at the beginning of a great exploration using micro/nanoengineered functional biomaterials for both fundamental biology study and clinical and biomedical applications such as regenerative medicine and drug screening. In this review, we present an overview of state of the art micro/nanoengineered functional biomaterials that can control precisely individual aspects of cell-microenvironment interactions and highlight them as well-controlled platforms for mechanistic studies of mechano-sensitive and -responsive cellular behaviors and integrative biology research. We also discuss the recent exciting trend where micro/nanoengineered biomaterials are integrated into miniaturized biological and biomimetic systems for dynamic multiparametric microenvironmental control of emergent and integrated cellular behaviors. The impact of integrated micro/nanoengineered functional biomaterials for future in vitro studies of regenerative medicine, cell biology, as well as human development and disease models are discussed. PMID:24339188

Physical properties of root cementum: Part 26. Effects of micro-osteoperforations on orthodontic root resorption: A microcomputed tomography study.

PubMed

Chan, Emmanuel; Dalci, Oyku; Petocz, Peter; Papadopoulou, Alexandra K; Darendeliler, M Ali

2018-02-01

Studies have demonstrated the potential efficacy of micro-osteoperforations in accelerating tooth movement by amplifying the expression of inflammatory markers. The aim of this investigation was to examine the effects of micro-osteoperforations on orthodontic root resorption with microcomputed tomography. This prospective controlled clinical trial involved 20 subjects requiring extraction of the maxillary first premolars as part of their orthodontic treatment. A buccal tipping force of 150 g was applied to both premolars. Using the Propel appliance (Propel Orthodontics, San Jose, Calif), micro-osteoperforations were applied at a depth of 5 mm on the mesial and distal aspects in the midroot region of the experimental side of the first premolar root; the contralateral side served as the control. After 28 days, both premolars were extracted. The teeth were scanned under microcomputed tomography, and the volumes of root resorption craters were calculated and compared. Premolars treated with micro-osteoperforation exhibited significantly greater average total amounts of root resorption than did the control teeth (0.576 vs 0.406 mm 3 ). The total average volumetric root loss of premolars treated with micro-osteoperforation was 42% greater than that of the control teeth. This 28-day trial showed that micro-osteoperforations resulted in greater orthodontic root resorption. However, these results should be verified in patients who are undergoing full-length orthodontic treatment. Copyright © 2017 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.

Theory for controlling individual self-propelled micro-swimmers by photon nudging I: directed transport.

PubMed

Selmke, Markus; Khadka, Utsab; Bregulla, Andreas P; Cichos, Frank; Yang, Haw

2018-04-18

Photon nudging is a new experimental method which enables the force-free manipulation and localization of individual self-propelled artificial micro-swimmers in fluidic environments. It uses a weak laser to stochastically and adaptively turn on and off the swimmer's propulsion when the swimmer, through rotational diffusion, points towards or away from its target, respectively. This contribution presents a theoretical framework for the statistics of both 2D and 3D controls. The main results are: the on- and off-time distributions for the controlling laser, the arrival time statistics for the swimmer to reach a remote target, and how the experimentally accessible control parameters influence the control, e.g., the optimal acceptance angle for directed transport. The results are general in that they are independent of the propulsion or the actuation mechanisms. They provide a concrete physical picture for how a single artificial micro-swimmer could be navigated under thermal fluctuations-insights that could also be useful for understanding biological micro-swimmers.

Dehydration triggers differential microRNA expression in Xenopus laevis brain.

PubMed

Luu, Bryan E; Storey, Kenneth B

2015-11-15

African clawed frogs, Xenopus laevis, although primarily aquatic, have a high tolerance for dehydration, being capable of withstanding the loss of up to 32-35% of total water body water. Recent studies have shown that microRNAs play a role in the response to dehydration by the liver, kidney and ventral skin of X. laevis. MicroRNAs act by modulating the expression of mRNA transcripts, thereby affecting diverse biochemical pathways. In this study, 43 microRNAs were assessed in frog brains comparing control and dehydrated (31.2±0.83% of total body water lost) conditions. MicroRNAs of interest were measured using a modified protocol which employs polyadenylation of microRNAs prior to reverse transcription and qPCR. Twelve microRNAs that showed a significant decrease in expression (to 41-77% of control levels) in brains from dehydrated frogs (xla-miR-15a, -150, -181a, -191, -211, -218, -219b, -30c, -30e, -31, -34a, and -34b) were identified. Genomic analysis showed that the sequences of these dehydration-responsive microRNAs were highly conserved as compared with the comparable microRNAs of mice (91-100%). Suppression of these microRNAs implies that translation of the mRNA transcripts under their control could be enhanced in response to dehydration. Bioinformatic analysis using the DIANA miRPath program (v.2.0) predicted the top two KEGG pathways that these microRNAs collectively regulate: 1. Axon guidance, and 2. Long-term potentiation. Previous studies indicated that suppression of these microRNAs promotes neuroprotective pathways by increasing the expression of brain-derived neurotrophic factor and activating anti-apoptotic pathways. This suggests that similar actions may be triggered in X. laevis brains as a protective response to dehydration. Crown Copyright © 2015. Published by Elsevier B.V. All rights reserved.

Frequency-varying synchronous micro-vibration suppression for a MSFW with application of small-gain theorem

NASA Astrophysics Data System (ADS)

Peng, Cong; Fan, Yahong; Huang, Ziyuan; Han, Bangcheng; Fang, Jiancheng

2017-01-01

This paper presents a novel synchronous micro-vibration suppression method on the basis of the small gain theorem to reduce the frequency-varying synchronous micro-vibration forces for a magnetically suspended flywheel (MSFW). The proposed synchronous micro-vibration suppression method not only eliminates the synchronous current fluctuations to force the rotor spinning around the inertia axis, but also considers the compensation caused by the displacement stiffness in the permanent-magnet (PM)-biased magnetic bearings. Moreover, the stability of the proposed control system is exactly analyzed by using small gain theorem. The effectiveness of the proposed micro-vibration suppression method is demonstrated via the direct measurement of the disturbance forces for a MSFW. The main merit of the proposed method is that it provides a simple and practical method in suppressing the frequency varying micro-vibration forces and preserving the nominal performance of the baseline control system.

A normally-closed piezoelectric micro-valve with flexible stopper

NASA Astrophysics Data System (ADS)

Chen, Song; Lu, Song; Liu, Yong; Wang, Jiantao; Tian, Xiaochao; Liu, Guojun; Yang, Zhigang

2016-04-01

In the field of controlled drug delivery system, there are still many problems on those reported micro-valves, such as the small opening height, unsatisfactory particle tolerance and high cost. To solve the above problems, a novel normally-closed piezoelectric micro-valve is presented in this paper. The micro-valve was driven by circular unimorph piezoelectric vibrator and natural rubber membrane with high elasticity was used as the valve stopper. The small axial displacement of piezoelectric vibrator can be converted into a large stroke of valve stopper based on hydraulic amplification mechanism. The experiment indicates that maximum hydraulic amplification ratio is up to 14, and the cut-off pressure of the micro-valve is 39kPa in the case of no working voltage. The presented micro valve has a large flow control range (ranging from 0 to 8.75mL/min).

MEMS deformable mirror for wavefront correction of large telescopes

NASA Astrophysics Data System (ADS)

Manhart, Sigmund; Vdovin, Gleb; Collings, Neil; Sodnik, Zoran; Nikolov, Susanne; Hupfer, Werner

2017-11-01

A 50 mm diameter membrane mirror was designed and manufactured at TU Delft. It is made from bulk silicon by micromachining - a technology primarily used for micro-electromechanical systems (MEMS). The mirror unit is equipped with 39 actuator electrodes and can be electrostatically deformed to correct wavefront errors in optical imaging systems. Performance tests on the deformable mirror were carried out at Astrium GmbH using a breadboard setup with a wavefront sensor and a closed-loop control system. It was found that the deformable membrane mirror is well suited for correction of low order wavefront errors as they must be expected in lightweighted space telescopes.

[Clinical effects of micro-implant and traditional anchorage in orthodontic treatments].

PubMed

Qian, Yi; Zhou, Hua-Jie; Wu, Jian-Hua

2017-06-01

To analyze the value of micro-implant and traditional anchorage in the treatment of malocclusion. From Jan 2015 to Jan 2016, 20 cases with malocclusion were randomly divided into control group(10) and experimental group (10). A comparison was conducted between the control group, in which traditional anchorage was used and the experimental group, in which micro-implant anchorage was adopted. The data were analyzed with SPSS 17.0 software package. There was significant difference of U1-NA, L1-NB, U1-APg, U6-PtPNS between the 2 groups(P<0.05). Adverse reaction was not found in the control group, but 8% in the experimental group(P<0.05). Micro-implant anchorage can improve overjet relation of the anterior teeth and effect of orthodontic treatment.

Optimal Micro-Scale Secondary Flow Control for the Management of High Cycle Fatigue and Distortion in Compact Inlet Diffusers

NASA Technical Reports Server (NTRS)

Anderson, Bernhard H.; Keller, Dennis J.

2002-01-01

The purpose of this study on micro-scale secondary flow control (MSFC) is to study the aerodynamic behavior of micro-vane effectors through their factor (i.e., the design variable) interactions and to demonstrate how these statistical interactions, when brought together in an optimal manner, determine design robustness. The term micro-scale indicates the vane effectors are small in comparison to the local boundary layer height. Robustness in this situation means that it is possible to design fixed MSFC robust installation (i.e.. open loop) which operates well over the range of mission variables and is only marginally different from adaptive (i.e., closed loop) installation design, which would require a control system. The inherent robustness of MSFC micro-vane effector installation designs comes about because of their natural aerodynamic characteristics and the manner in which these characteristics are brought together in an optimal manner through a structured Response Surface Methodology design process.

Structural coloration of metallic surfaces with micro/nano-structures induced by elliptical vibration texturing

NASA Astrophysics Data System (ADS)

Yang, Yang; Pan, Yayue; Guo, Ping

2017-04-01

Creating orderly periodic micro/nano-structures on metallic surfaces, or structural coloration, for control of surface apparent color and optical reflectivity has been an exciting research topic over the years. The direct applications of structural coloration include color marking, display devices, and invisibility cloak. This paper presents an efficient method to colorize metallic surfaces with periodic micro/nano-gratings using elliptical vibration texturing. When the tool vibration is coupled with a constant cutting velocity, controlled periodic ripples can be generated due to the overlapping tool trajectory. These periodic ripples with a wavelength near visible spectrum can act as micro-gratings to introduce iridescent colors. The proposed technique also provides a flexible method for color marking of metallic surfaces with arbitrary patterns and images by precise control of the spacing distance and orientation of induced micro/nano-ripples. Theoretical analysis and experimental results are given to demonstrate structural coloration of metals by a direct mechanical machining technique.

Integrated Power Source Grant

NASA Technical Reports Server (NTRS)

2001-01-01

Traditional spacecraft power systems incorporate a solar array energy source, an energy storage element (battery), and battery charge control and bus voltage regulation electronics to provide continuous electrical power for spacecraft systems and instruments. Dedicated power conditioning components provide limited fault isolation between systems and instruments, while a centralized power-switching unit provides spacecraft load control. Battery undervoltage conditions are detected by the spacecraft processor, which removes fault conditions and non-critical loads before permanent battery damage can occur. Cost effective operation of a micro-sat constellation requires a fault tolerant spacecraft architecture that minimizes on-orbit operational costs by permitting autonomous reconfiguration in response to unexpected fault conditions. A new micro-sat power system architecture that enhances spacecraft fault tolerance and improves power system survivability by continuously managing the battery charge and discharge processes on a cell-by-cell basis has been developed. This architecture is based on the Integrated Power Source (US patent 5644207), which integrates dual junction solar cells, Lithium Ion battery cells, and processor based charge control electronics into a structural panel that can be deployed or used to form a portion of the outer shell of a micro-spacecraft. The first generation Integrated Power Source is configured as a one inch thick panel in which prismatic Lithium Ion battery cells are arranged in a 3x7 matrix (26VDC) and a 3x1 matrix (3.7VDC) to provide the required output voltages and load currents. A multi-layer structure holds the battery cells, as well as the thermal insulators that are necessary to protect the Lithium Ion battery cells from the extreme temperatures of the solar cell layer. Independent thermal radiators, located on the back of the panel, are dedicated to the solar cell array, the electronics, and the battery cell array. In deployed panel applications, these radiators maintain the battery cells in an appropriate operational temperature range.

Lower neurocognitive function in U-2 pilots

PubMed Central

Tate, David F.; Wood, Joe; Sladky, John H.; McDonald, Kent; Sherman, Paul M.; Kawano, Elaine S.; Rowland, Laura M.; Patel, Beenish; Wright, Susan N.; Hong, Elliot; Rasmussen, Jennifer; Willis, Adam M.; Kochunov, Peter V.

2014-01-01

Objective: Determine whether United States Air Force (USAF) U-2 pilots (U2Ps) with occupational exposure to repeated hypobaria had lower neurocognitive performance compared to pilots without repeated hypobaric exposure and whether U2P neurocognitive performance correlated with white matter hyperintensity (WMH) burden. Methods: We collected Multidimensional Aptitude Battery–II (MAB-II) and MicroCog: Assessment of Cognitive Functioning (MicroCog) neurocognitive data on USAF U2Ps with a history of repeated occupational exposure to hypobaria and compared these with control data collected from USAF pilots (AFPs) without repeated hypobaric exposure (U2Ps/AFPs MAB-II 87/83; MicroCog 93/80). Additional comparisons were performed between U2Ps with high vs low WMH burden. Results: U2Ps with repeated hypobaric exposure had significantly lower scores than control pilots on reasoning/calculation (U2Ps/AFPs 99.4/106.5), memory (105.5/110.9), information processing accuracy (102.1/105.8), and general cognitive functioning (103.5/108.5). In addition, U2Ps with high whole-brain WMH count showed significantly lower scores on reasoning/calculation (high/low 96.8/104.1), memory (102.9/110.2), general cognitive functioning (101.5/107.2), and general cognitive proficiency (103.6/108.8) than U2Ps with low WMH burden (high/low WMH mean volume 0.213/0.003 cm3 and mean count 14.2/0.4). Conclusion: In these otherwise healthy, highly functioning individuals, pilots with occupational exposure to repeated hypobaria demonstrated lower neurocognitive performance, albeit demonstrable on only some tests, than pilots without repeated exposure. Furthermore, within the U2P population, higher WMH burden was associated with lower neurocognitive test performance. Hypobaric exposure may be a risk factor for subtle changes in neurocognition. PMID:25008397

[The project and simulation of a compositive miniature spectrum instrument based on the array of Fabry-Perot cavity].

PubMed

Wen, Zhi-yu; Chen, Gang; Wang, Jian-guo

2006-10-01

This paper advances a kind of micro-spectrometer based on Fabry-Perot cavity's character of filtering the waves. The basic structure of the micro-spectrometer is the array of Fabry-Perot cavity which contains many different lengths of cavity on the substrate of silicon, consequently the authors can achieve the detection at several wavelengths simultaneously. The unit of probing is a Fabry-Perot cavity made up of the substrate of silicon-metal film-silicon dioxide layer-metal film. The authors carried out the corresponding simulation. In the basic structure of aluminum film(14 nm)-silicon dioxide layer-silver film(39 nm), the resolution can reach 15 nm. When the area of a unit of probing is 0.14 mm x 0.14 mm only, it can reach the luminous flux of miniature grating spectrum instrument (the minimum volume in the order of cm), but the volume of the part of spectrum detection is only of the order of mm. The design size of the micro-spectrometer is a few millimeters. Furthermore it has no movable parts and could detect several wavelengths at the same time. It is possible to fabricate such micro-spectrometer through existing processing methods of IC technology.

Temperature-insensitive refractive index sensing by use of micro Fabry-Pérot cavity based on simplified hollow-core photonic crystal fiber.

PubMed

Wang, Ying; Wang, D N; Liao, C R; Hu, Tianyi; Guo, Jiangtao; Wei, Huifeng

2013-02-01

A temperature-insensitive micro Fabry-Pérot (FP) cavity based on simplified hollow-core (SHC) photonic crystal fiber (PCF) is demonstrated. Such a device is fabricated by splicing a section of SHC PCF with single mode fibers at both cleaved ends. An extremely low temperature sensitivity of ~0.273 pm/°C is obtained between room temperature and 900°C. By drilling vertical micro-channels using a femtosecond laser, the micro FP cavity can be filled with liquids and functions as a sensitive refractometer and the refractive index sensitivity obtained is ~851.3 nm/RIU (refractive index unit), which indicates an ultra low temperature cross-sensitivity of ~3.2×10(-7) RIU/°C.

Study on improved Ip-iq APF control algorithm and its application in micro grid

NASA Astrophysics Data System (ADS)

Xie, Xifeng; Shi, Hua; Deng, Haiyingv

2018-01-01

In order to enhance the tracking velocity and accuracy of harmonic detection by ip-iq algorithm, a novel ip-iq control algorithm based on the Instantaneous reactive power theory is presented, the improved algorithm adds the lead correction link to adjust the zero point of the detection system, the Fuzzy Self-Tuning Adaptive PI control is introduced to dynamically adjust the DC-link Voltage, which meets the requirement of the harmonic compensation of the micro grid. Simulation and experimental results verify the proposed method is feasible and effective in micro grid.

A New Individually Addressable Micro-LED Array for Photogenetic Neural Stimulation.

PubMed

McGovern, B; Berlinguer Palmini, R; Grossman, N; Drakakis, E; Poher, V; Neil, M A A; Degenaar, P

2010-12-01

Here, we demonstrate the use of a micro light emitting diode (LED) array as a powerful tool for complex spatiotemporal control of photosensitized neurons. The array can generate arbitrary, 2-D, excitation patterns with millisecond and micrometer resolution. In particular, we describe an active matrix control address system to allow simultaneous control of 256 individual micro LEDs. We present the system optically integrated into a microscope environment and patch clamp electrophysiology. The results show that the emitters have sufficient radiance at the required wavelength to stimulate neurons expressing channelrhodopsin-2 (ChR2).

Engineering challenges of BioNEMS: the integration of microfluidics, micro- and nanodevices, models and external control for systems biology.

PubMed

Wikswo, J P; Prokop, A; Baudenbacher, F; Cliffel, D; Csukas, B; Velkovsky, M

2006-08-01

Systems biology, i.e. quantitative, postgenomic, postproteomic, dynamic, multiscale physiology, addresses in an integrative, quantitative manner the shockwave of genetic and proteomic information using computer models that may eventually have 10(6) dynamic variables with non-linear interactions. Historically, single biological measurements are made over minutes, suggesting the challenge of specifying 10(6) model parameters. Except for fluorescence and micro-electrode recordings, most cellular measurements have inadequate bandwidth to discern the time course of critical intracellular biochemical events. Micro-array expression profiles of thousands of genes cannot determine quantitative dynamic cellular signalling and metabolic variables. Major gaps must be bridged between the computational vision and experimental reality. The analysis of cellular signalling dynamics and control requires, first, micro- and nano-instruments that measure simultaneously multiple extracellular and intracellular variables with sufficient bandwidth; secondly, the ability to open existing internal control and signalling loops; thirdly, external BioMEMS micro-actuators that provide high bandwidth feedback and externally addressable intracellular nano-actuators; and, fourthly, real-time, closed-loop, single-cell control algorithms. The unravelling of the nested and coupled nature of cellular control loops requires simultaneous recording of multiple single-cell signatures. Externally controlled nano-actuators, needed to effect changes in the biochemical, mechanical and electrical environment both outside and inside the cell, will provide a major impetus for nanoscience.

The use of surface electromyography as a tool in differentiating temporomandibular disorders from neck disorders.

PubMed

Ferrario, Virgilio F; Tartaglia, Gianluca M; Luraghi, Francesca E; Sforza, Chiarella

2007-11-01

The aim of this study was to assess the electromyographic characteristics of the masticatory muscles (masseter and temporalis) of patients with either "temporomandibular joint disorder" or "neck pain". Surface electromyography of the right and left masseter and temporalis muscles was performed during maximum teeth clenching in 38 patients aged 21-67 years who had either (a) temporomandibular joint disorder (24 patients); (b) "neck pain" (13 patients). Ninety-five control, healthy subjects were also examined. During clenching, standardized total muscle activities (electromyographic potentials over time) were significantly different in the three groups: 75 microV/microVs% in the temporomandibular joint disorder patients, 124 microV/microVs% in the neck pain patients, and 95 microV/microVs% in the control subjects (analysis of variance, P<0.001). The temporomandibular joint disorder patients also had significantly (P<0.001) more asymmetric muscle potentials (78%) than either neck pain patients (87%) or control subjects (92%). A linear discriminant function analysis allowed a significant separation between the two patient groups, with a single patient error of 18.2%. Surface electromyographic analysis during clenching allowed to differentiate between patients with a temporomandibular joint disorder and patients with a neck pain problem.

Clinical application of micro-implant anchorage in initial orthodontic retraction.

PubMed

Wahabuddin, Shaji; Mascarenhas, Rohan; Iqbal, Mahamad; Husain, Akhter

2015-02-01

Micro-implant is a device that is temporarily fixed to bone for the purpose of enhancing orthodontic anchorage either by supporting the teeth of the reactive unit or by obviating the need for the reactive unit altogether, and which is subsequently removed after use. The purpose of this study was to evaluate the clinical efficiency of micro-implants in reinforcing anchorage during the initial retraction of anterior teeth, check the rate of initial retraction for 8 weeks, and assess the stability of micro-implants during this period. Eighteen micro-implants were placed (10 in the maxilla and 8 in the mandible) and immediately loaded with 200-250 g of force using 9-mm closed coil Nitinol springs. The amount of space closure was measured every 2 weeks until the eighth week. Cephalometric measurements were made at the end of the study to evaluate anchor loss, if any. Micro-implant stability was also assessed. The rate of initial retraction in the maxilla at the end of 8 weeks was 1.65 mm/quadrant and 1.51 mm/quadrant in the mandible. The amount of retraction on the left side of the arches was 1.66 mm/quadrant and 1.49 mm/quadrant on the right side. The average initial retraction for both arches per month was 0.78 mm. An anchor loss of 0.1 mm (0.06%) was observed in the maxilla while no mandibular anchor loss was recorded. The rate of initial retraction observed in the maxilla was more than that achieved in the mandible. Initial retraction was also more on the left side of the arches. There was no anchor loss in the mandible. The micro-implant-reinforced anchorage was helpful in minimizing anchor loss and accepted heavy traction forces but did not bring about a faster rate of retraction.

Imaging the Human Aqueous Humor Outflow Pathway in Human Eyes by Three Dimensional Micro-Computed Tomography (3D micro-CT)

PubMed Central

Hann, Cheryl R.; Bentley, Michael D.; Vercnocke, Andrew; Ritman, Erik L.; Fautsch, Michael P.

2011-01-01

The site of outflow resistance leading to elevated intraocular pressure in primary open angle glaucoma is believed to be located in the region of Schlemm’s canal inner wall endothelium, its basement membrane and the adjacent juxtacanalicular tissue. Evidence also suggests collector channels and intrascleral vessels may have a role in intraocular pressure in both normal and glaucoma eyes. Traditional imaging modalities limit the ability to view both proximal and distal portions of the trabecular outflow pathway as a single unit. In this study, we examined the effectiveness of three-dimensional micro-computed tomography (3D micro-CT) as a potential method to view the trabecular outflow pathway. Two normal human eyes were used: one immersion fixed in 4% paraformaldehyde, and one with anterior chamber perfusion at 10 mmHg followed by perfusion fixation in 4% paraformaldehyde/2% glutaraldehyde. Both eyes were postfixed in 1% osmium tetroxide, and scanned with 3D micro-CT at 2 µm or 5 µm voxel resolution. In the immersion fixed eye, 24 collector channels were identified with an average orifice size of 27.5 ± 5 µm. In comparison, the perfusion fixed eye had 29 collector channels with a mean orifice size of 40.5 ± 13 µm. Collector channels were not evenly dispersed around the circumference of the eye. There was no significant difference in the length of Schlemm’s canal in the immersed versus the perfused eye (33.2 versus 35.1 mm). Structures, locations and size measurements identified by 3D micro-CT were confirmed by correlative light microscopy. These findings confirm 3D micro-CT can be used effectively for the non-invasive examination of the trabecular meshwork, Schlemm’s canal, collector channels and intrascleral vasculature that comprise the distal outflow pathway. This imaging modality will be useful for noninvasive study of the role of the trabecular outflow pathway as a whole unit. PMID:21187085

Imaging the Aqueous Humor Outflow Pathway in Human Eyes by Three-dimensional Micro-computed Tomography (3D micro-CT)

DOE Office of Scientific and Technical Information (OSTI.GOV)

C Hann; M Bentley; A Vercnocke

2011-12-31

The site of outflow resistance leading to elevated intraocular pressure in primary open-angle glaucoma is believed to be located in the region of Schlemm's canal inner wall endothelium, its basement membrane and the adjacent juxtacanalicular tissue. Evidence also suggests collector channels and intrascleral vessels may have a role in intraocular pressure in both normal and glaucoma eyes. Traditional imaging modalities limit the ability to view both proximal and distal portions of the trabecular outflow pathway as a single unit. In this study, we examined the effectiveness of three-dimensional micro-computed tomography (3D micro-CT) as a potential method to view the trabecularmore » outflow pathway. Two normal human eyes were used: one immersion fixed in 4% paraformaldehyde and one with anterior chamber perfusion at 10 mmHg followed by perfusion fixation in 4% paraformaldehyde/2% glutaraldehyde. Both eyes were postfixed in 1% osmium tetroxide and scanned with 3D micro-CT at 2 {mu}m or 5 {mu}m voxel resolution. In the immersion fixed eye, 24 collector channels were identified with an average orifice size of 27.5 {+-} 5 {mu}m. In comparison, the perfusion fixed eye had 29 collector channels with a mean orifice size of 40.5 {+-} 13 {mu}m. Collector channels were not evenly dispersed around the circumference of the eye. There was no significant difference in the length of Schlemm's canal in the immersed versus the perfused eye (33.2 versus 35.1 mm). Structures, locations and size measurements identified by 3D micro-CT were confirmed by correlative light microscopy. These findings confirm 3D micro-CT can be used effectively for the non-invasive examination of the trabecular meshwork, Schlemm's canal, collector channels and intrascleral vasculature that comprise the distal outflow pathway. This imaging modality will be useful for non-invasive study of the role of the trabecular outflow pathway as a whole unit.« less

Effects of fenoterol on beta-adrenoceptor and muscarinic M2 receptor function in bovine tracheal smooth muscle.

PubMed

De Vries, B; Roffel, A F; Kooistra, J M; Meurs, H; Zaagsma, J

2001-05-11

Prolonged (18 h) incubation of isolated bovine tracheal smooth muscle with the beta2-adrenoceptor agonist fenoterol (10 microM) induced desensitization of isoprenaline-induced adenylyl cyclase activity in bovine tracheal smooth muscle membranes, characterized by a 25% decrease in maximal effect (Emax) (P < 0.05), while the sensitivity to the agonist (pEC50) was unchanged. The Emax value of isoprenaline-induced smooth muscle relaxation of submaximal methacholine-induced contractile tones was similarly reduced by about 25% (P < 0.001), while the pEC50 value was diminished by 1.0 log unit (P < 0.001). As determined by 30 microM gallamine-induced muscarinic M2 receptor antagonism and pertussis toxin-induced inactivation of G(i alpha), muscarinic M2 receptor-mediated functional antagonism did not play a role in isoprenaline-induced relaxation of bovine tracheal smooth muscle contracted by methacholine, both in control and in 18-h fenoterol-treated tissue. In line with these observations, we found no enhanced muscarinic M2 receptor-mediated inhibition of 1 microM forskolin-stimulated adenylyl cyclase activity after 18-h fenoterol treatment. These data indicate that 18-h fenoterol treatment of bovine tracheal smooth muscle induces beta2-adrenoceptor desensitization and reduced functional antagonism of methacholine-induced contraction by beta-adrenoceptor agonists, without a change of muscarinic M2 receptor function.

Cementation of Glass-Ceramic Posterior Restorations: A Systematic Review

PubMed Central

van den Breemer, Carline R. G.; Gresnigt, Marco M. M.; Cune, Marco S.

2015-01-01

Aim. The aim of this comprehensive review is to systematically organize the current knowledge regarding the cementation of glass-ceramic materials and restorations, with an additional focus on the benefits of Immediate Dentin Sealing (IDS). Materials and Methods. An extensive literature search concerning the cementation of single-unit glass-ceramic posterior restorations was conducted in the databases of MEDLINE (Pubmed), CENTRAL (Cochrane Central Register of Controlled Trials), and EMBASE. To be considered for inclusion, in vitro and in vivo studies should compare different cementation regimes involving a “glass-ceramic/cement/human tooth” complex. Results and Conclusions. 88 studies were included in total. The in vitro data were organized according to the following topics: (micro)shear and (micro)tensile bond strength, fracture strength, and marginal gap and integrity. For in vivo studies survival and quality of survival were considered. In vitro studies showed that adhesive systems (3-step, etch-and-rinse) result in the best (micro)shear bond strength values compared to self-adhesive and self-etch systems when luting glass-ceramic substrates to human dentin. The highest fracture strength is obtained with adhesive cements in particular. No marked clinical preference for one specific procedure could be demonstrated on the basis of the reviewed literature. The possible merits of IDS are most convincingly illustrated by the favorable microtensile bond strengths. No clinical studies regarding IDS were found. PMID:26557651

The generation of concentration gradients using electroosmotic flow in micro reactors allowing stereoselective chemical synthesis.

PubMed

Skelton, V; Greenway, G M; Haswell, S J; Styring, P; Morgan, D O; Warrington, B H; Wong, S Y

2001-01-01

The stereoselective control of chemical reactions has been achieved by applying electrical fields in a micro reactor generating controlled concentration gradients of the reagent streams. The chemistry based upon well-established Wittig synthesis was carried out in a micro reactor device fabricated in borosilicate glass using photolithographic and wet etching techniques. The selectivity of the cis (Z) to trans (E) isomeric ratio in the product synthesised was controlled by varying the applied voltages to the reagent reservoirs within the micro reactor. This subsequently altered the relative reagent concentrations within the device resulting in Z/E ratios in the range 0.57-5.21. By comparison, a traditional batch method based on the same reaction length, concentration, solvent and stoichiometry (i.e., 1.0:1.5:1.0 reagent ratios) gave a Z/E in the range 2.8-3.0. However, when the stoichiometric ratios were varied up to ten times as much, the Z/E ratios varied in accordance to the micro reactor i.e., when the aldehyde is in excess, the Z isomer predominates whereas when the aldehyde is in low concentrations, the E isomer is the more favourable form. Thus indicating that localised concentration gradients generated by careful flow control due to the diffusion limited non-turbulent mixing regime within a micro reactor, leads to the observed stereo selectivity for the cis and trans isomers.

Toward autonomous avian-inspired grasping for micro aerial vehicles.

PubMed

Thomas, Justin; Loianno, Giuseppe; Polin, Joseph; Sreenath, Koushil; Kumar, Vijay

2014-06-01

Micro aerial vehicles, particularly quadrotors, have been used in a wide range of applications. However, the literature on aerial manipulation and grasping is limited and the work is based on quasi-static models. In this paper, we draw inspiration from agile, fast-moving birds such as raptors, that are able to capture moving prey on the ground or in water, and develop similar capabilities for quadrotors. We address dynamic grasping, an approach to prehensile grasping in which the dynamics of the robot and its gripper are significant and must be explicitly modeled and controlled for successful execution. Dynamic grasping is relevant for fast pick-and-place operations, transportation and delivery of objects, and placing or retrieving sensors. We show how this capability can be realized (a) using a motion capture system and (b) without external sensors relying only on onboard sensors. In both cases we describe the dynamic model, and trajectory planning and control algorithms. In particular, we present a methodology for flying and grasping a cylindrical object using feedback from a monocular camera and an inertial measurement unit onboard the aerial robot. This is accomplished by mapping the dynamics of the quadrotor to a level virtual image plane, which in turn enables dynamically-feasible trajectory planning for image features in the image space, and a vision-based controller with guaranteed convergence properties. We also present experimental results obtained with a quadrotor equipped with an articulated gripper to illustrate both approaches.

Integrated micro/nanoengineered functional biomaterials for cell mechanics and mechanobiology: a materials perspective.

PubMed

Shao, Yue; Fu, Jianping

2014-03-12

The rapid development of micro/nanoengineered functional biomaterials in the last two decades has empowered materials scientists and bioengineers to precisely control different aspects of the in vitro cell microenvironment. Following a philosophy of reductionism, many studies using synthetic functional biomaterials have revealed instructive roles of individual extracellular biophysical and biochemical cues in regulating cellular behaviors. Development of integrated micro/nanoengineered functional biomaterials to study complex and emergent biological phenomena has also thrived rapidly in recent years, revealing adaptive and integrated cellular behaviors closely relevant to human physiological and pathological conditions. Working at the interface between materials science and engineering, biology, and medicine, we are now at the beginning of a great exploration using micro/nanoengineered functional biomaterials for both fundamental biology study and clinical and biomedical applications such as regenerative medicine and drug screening. In this review, an overview of state of the art micro/nanoengineered functional biomaterials that can control precisely individual aspects of cell-microenvironment interactions is presented and they are highlighted them as well-controlled platforms for mechanistic studies of mechano-sensitive and -responsive cellular behaviors and integrative biology research. The recent exciting trend where micro/nanoengineered biomaterials are integrated into miniaturized biological and biomimetic systems for dynamic multiparametric microenvironmental control of emergent and integrated cellular behaviors is also discussed. The impact of integrated micro/nanoengineered functional biomaterials for future in vitro studies of regenerative medicine, cell biology, as well as human development and disease models are discussed. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Systematical analysis of cutaneous squamous cell carcinoma network of microRNAs, transcription factors, and target and host genes.

PubMed

Wang, Ning; Xu, Zhi-Wen; Wang, Kun-Hao

2014-01-01

MicroRNAs (miRNAs) are small non-coding RNA molecules found in multicellular eukaryotes which are implicated in development of cancer, including cutaneous squamous cell carcinoma (cSCC). Expression is controlled by transcription factors (TFs) that bind to specific DNA sequences, thereby controlling the flow (or transcription) of genetic information from DNA to messenger RNA. Interactions result in biological signal control networks. Molecular components involved in cSCC were here assembled at abnormally expressed, related and global levels. Networks at these three levels were constructed with corresponding biological factors in term of interactions between miRNAs and target genes, TFs and miRNAs, and host genes and miRNAs. Up/down regulation or mutation of the factors were considered in the context of the regulation and significant patterns were extracted. Participants of the networks were evaluated based on their expression and regulation of other factors. Sub-networks with two core TFs, TP53 and EIF2C2, as the centers are identified. These share self-adapt feedback regulation in which a mutual restraint exists. Up or down regulation of certain genes and miRNAs are discussed. Some, for example the expression of MMP13, were in line with expectation while others, including FGFR3, need further investigation of their unexpected behavior. The present research suggests that dozens of components, miRNAs, TFs, target genes and host genes included, unite as networks through their regulation to function systematically in human cSCC. Networks built under the currently available sources provide critical signal controlling pathways and frequent patterns. Inappropriate controlling signal flow from abnormal expression of key TFs may push the system into an incontrollable situation and therefore contributes to cSCC development.

Serum MicroRNAs as Potential Biomarkers for Early Diagnosis of Hepatitis C Virus-Related Hepatocellular Carcinoma in Egyptian Patients

PubMed Central

Motawi, Tarek K.; Shaker, Olfat G.; El-Maraghy, Shohda A.; Senousy, Mahmoud A.

2015-01-01

Circulating microRNAs are deregulated in liver fibrosis and hepatocellular carcinoma (HCC) and are candidate biomarkers. This study investigated the potential of serum microRNAs; miR-19a, miR-296, miR-130a, miR-195, miR-192, miR-34a, and miR-146a as early diagnostic biomarkers for hepatitis C virus (HCV)-related HCC. As how these microRNAs change during liver fibrosis progression is not clear, we explored their serum levels during fibrosis progression in HCV-associated chronic liver disease (CLD) and if they could serve as non-invasive biomarkers for fibrosis progression to HCC. 112 Egyptian HCV-HCC patients, 125 non-malignant HCV-CLD patients, and 42 healthy controls were included. CLD patients were subdivided according to Metavir fibrosis-scoring. Serum microRNAs were measured by qRT-PCR custom array. Serum microRNAs were deregulated in HCC versus controls, and except miR-130a, they were differentially expressed between HCC and CLD or late fibrosis (F3-F4) subgroup. Serum microRNAs were not significantly different between individual fibrosis-stages or between F1-F2 (early/moderate fibrosis) and F3-F4. Only miR-19a was significantly downregulated from liver fibrosis (F1-F3) to cirrhosis (F4) to HCC. Individual microRNAs discriminated HCC from controls, and except miR-130a, they distinguished HCC from CLD or F3-F4 patients by receiver-operating-characteristic analysis. Multivariate logistic analysis revealed a panel of four microRNAs (miR-19a, miR-195, miR-192, and miR-146a) with high diagnostic accuracy for HCC (AUC = 0.946). The microRNA panel also discriminated HCC from controls (AUC = 0.949), CLD (AUC = 0.945), and F3-F4 (AUC = 0.955). Studied microRNAs were positively correlated in HCC group. miR-19a and miR-34a were correlated with portal vein thrombosis and HCC staging scores, respectively. In conclusion, studied microRNAs, but not miR-130a, could serve as potential early biomarkers for HCC in high-risk groups, with miR-19a as a biomarker for liver fibrosis progression to cirrhosis to HCC. We identified a panel of four serum microRNAs with high accuracy in HCC diagnosis. Additional studies are required to confirm this panel and test its prognostic significance. PMID:26352740

Stabilization and control of quad-rotor helicopter using a smartphone device

NASA Astrophysics Data System (ADS)

Desai, Alok; Lee, Dah-Jye; Moore, Jason; Chang, Yung-Ping

2013-01-01

In recent years, autonomous, micro-unmanned aerial vehicles (micro-UAVs), or more specifically hovering micro- UAVs, have proven suitable for many promising applications such as unknown environment exploration and search and rescue operations. The early versions of UAVs had no on-board control capabilities, and were difficult for manual control from a ground station. Many UAVs now are equipped with on-board control systems that reduce the amount of control required from the ground-station operator. However, the limitations on payload, power consumption and control without human interference remain the biggest challenges. This paper proposes to use a smartphone as the sole computational device to stabilize and control a quad-rotor. The goal is to use the readily available sensors in a smartphone such as the GPS, the accelerometer, the rate-gyros, and the camera to support vision-related tasks such as flight stabilization, estimation of the height above ground, target tracking, obstacle detection, and surveillance. We use a quad-rotor platform that has been built in the Robotic Vision Lab at Brigham Young University for our development and experiments. An Android smartphone is connected through the USB port to an external hardware that has a microprocessor and circuitries to generate pulse-width modulation signals to control the brushless servomotors on the quad-rotor. The high-resolution camera on the smartphone is used to detect and track features to maintain a desired altitude level. The vision algorithms implemented include template matching, Harris feature detector, RANSAC similarity-constrained homography, and color segmentation. Other sensors are used to control yaw, pitch, and roll of the quad-rotor. This smartphone-based system is able to stabilize and control micro-UAVs and is ideal for micro-UAVs that have size, weight, and power limitations.

[Control of lead emissions from a battery repair shop in the city of Rio de Janeiro using the air as indicator].

PubMed

Quiterio, Simone Lorena; da Silva, Célia Regina Sousa; Arbilla, Graciela; Moreira, Maria de Fátima Ramos; Araújo, Ulisses César; Mattos, Rita de Cássia O da C; Santos, Luiz Sérgio Cardoso

2003-01-01

Lead levels in the air surrounding a battery repair shop (BRS) located in the Olaria neighborhood (Rio de Janeiro, Brazil) were monitored from April to July 1999. Most of the samples collected within 25 meters of the BRS exceeded the limit of 1.5 micro g.Pb.m-3 established by the United States Environmental Protection Agency (EPA). These results were published in a previous article (Quiterio et al., 2001). In the current study, installation of a Venture ventilation system and some changes in the operational procedure were proposed. After the modifications in the BRS, a new monitoring campaign was performed (August and September, 2000). Three points were selected for air collection, corresponding to the most critical points found in the previous assessments. The new concentrations ranged from 0.8 to 17.6 micro g.Pb.m-3, showing that lead emissions into the environment had decreased adequately. However, concentration at the chimney exhaust was still higher than EPA limits, and further improvements in the installations and procedures are definitely needed.

Development of a PPT for the EO-1 Spacecraft

NASA Technical Reports Server (NTRS)

Benson, Scott W.; Arrington, Lynn A.; Hoskins, W. Andrew; Meckel, Nicole J.

2000-01-01

A Pulsed Plasma Thruster (PPT) has been developed for use in a technology demonstration flight experiment on the Earth Observing 1 (EO-1) New Millennium Program mission. The thruster replaces the spacecraft pitch axis momentum wheel for control and momentum management during an experiment of a minimum three-day duration. The EO-1 PPT configuration is a combination of new technology and design heritage from similar systems flown in the 1970's and 1980's. Acceptance testing of the protoflight unit has validated readiness for flight, and integration with the spacecraft, including initial combined testing, has been completed. The thruster provides a range of capability from 90 microN-sec impulse bit at 650 sec specific impulse for 12 W input power, through 860 microN-sec impulse bit at 1400 see specific impulse for 70 W input power. Development of this thruster reinitiates technology research and development and re-establishes an industry base for production of flight hardware. This paper reviews the EO-1 PPT development, including technology selection, design and fabrication, acceptance testing, and initial spacecraft integration and test.

Frustules to fragments, diatoms to dust: How degradation of microfossil shape and microstructures can teach us how ice sheets work

USGS Publications Warehouse

Scherer, R.P.; Sjunneskog, C.M.; Iverson, M.R.; Hooyer, T.S.

2005-01-01

In a laboratory experiment we investigated micro- and nanoscale changes in fossil diatom valves and in the texture of diatomaceous sediments that result from ice sheet overburden and subglacial shearing. Our experiment included compression and shearing of Antarctic diatom-rich sediments in a ring shear device and comparison of experimental samples with natural glacial sediments from the Antarctic continental shelf. The purpose of the experiment is to establish objective criteria for analyzing subglacial processes and interpreting the origin of glacial-geologic features on the Antarctic continental shelf. We find distinct changes resulting from different glacial settings, with respect to whole diatom frustules, diatom micromorphology, and microtextural properties of sedimentary units. By providing constraints on subglacial shearing, these observations of genetically controlled micro- and nanoscale diatom structures and architecture are contributing to the understanding of large-scale glacial processes, aiding the development of models of modern ice sheet processes, and guiding interpretation of past ice sheet configurations. Copyright ?? 2005 American Scientific Publishers. All rights reserved.

The short-circuit current of the ileum, but not the colon, is altered in the streptozotocin diabetic rat.

PubMed

Forrest, Abigail; Makwana, Rajesh; Parsons, Mike

2006-02-01

Ion transport in control and streptozotocin-diabetic rat colon and ileum was studied using the Ussing chamber technique. No differences were observed between control and diabetic colonic mucosal short-circuit current under either basal or carbachol (100 nmol/L-1 micromol/L)-stimulated or prostaglandin E2 (100 nmol/L-1 micromol/L)-stimulated conditions. Similarly to colonic tissues, no differences in the short circuit current in either carbachol-stimulated or prostaglandin E2-stimulated tissues were observed between control and diabetic ileal mucosa. The basal diabetic ileal short circuit current (99.58 +/- 22.67 microA) was significantly greater than that of control ileal tissues (29.67 +/- 4.45 microA). This difference was abolished by the sodium-glucose-cotransporter inhibitor, phloridzin (50 micromol/L) (118.00 +/- 28.09 microA vs. 25.60 +/- 4.59 microA) and was also prevented by the replacement of glucose with mannitol in the buffer bathing the apical side of the tissue (control: 17.05 +/- 5.85 microA vs. 17.90 +/- 3.10 microA). Acetazolamide (450 micromol/L; a carbonic anhydrase inhibitor), amiloride, and bumetanide (100 micromol/L each; Na+-channel blockers), piroxicam (50 micromol/L; a COX1 cyclooxygenase inhibitor), and ouabain (1 mmol/L; a K+ transport inhibitor) had no effect on the basal short circuit current of either control or diabetic ileal tissues. This indicated that the alteration in the basal short circuit current of diabetic ileal tissues was due to a change in cellular glucose transport, whereas the evoked changes in short circuit current were unaffected by the diabetic state.

[Wireless Passive Body Sensor for Temperature Monitoring Using Near Field Communication Technology].

PubMed

Shi, Bo; Zhang, Li; Zhang, Genxuan; Tsau, Young; Zhang, Sai; Li, Lei

2017-01-01

In this study, we designed a wireless body temperature sensor (WBTS) based on near field communication (NFC) technology. Just attaching the WBTS to a mobile phone with NFC function, the real-time body temperature of human subjects can be acquired by an application program without seperate power supply. The WBTS is mainly composed of a digital body temperature probe (d-BTP), a NFC unit and an antenna. The d-BTP acquires and processes body temperature data through a micro control er, and the NFC unit and antenna are used for wireless energy transmission and data communication between the mobile phone and WBTS. UART communication protocol is used in the communication between the d-BTP and NFC unit, and data compression technique is adopted for improving transmission efficiency and decreasing power loss. In tests, the error of WBTS is ±0.1 oC, in range of 32 oC to 42 oC. The WBTS has advantages of high accuracy, low power loss, strong anti-interference ability, dispensation with independent power supply etc., and it can be integrated into wearable apparatuses for temperature monitoring and health management.

New paradigms in internal architecture design and freeform fabrication of tissue engineering porous scaffolds.

PubMed

Yoo, Dongjin

2012-07-01

Advanced additive manufacture (AM) techniques are now being developed to fabricate scaffolds with controlled internal pore architectures in the field of tissue engineering. In general, these techniques use a hybrid method which combines computer-aided design (CAD) with computer-aided manufacturing (CAM) tools to design and fabricate complicated three-dimensional (3D) scaffold models. The mathematical descriptions of micro-architectures along with the macro-structures of the 3D scaffold models are limited by current CAD technologies as well as by the difficulty of transferring the designed digital models to standard formats for fabrication. To overcome these difficulties, we have developed an efficient internal pore architecture design system based on triply periodic minimal surface (TPMS) unit cell libraries and associated computational methods to assemble TPMS unit cells into an entire scaffold model. In addition, we have developed a process planning technique based on TPMS internal architecture pattern of unit cells to generate tool paths for freeform fabrication of tissue engineering porous scaffolds. Copyright © 2012 IPEM. Published by Elsevier Ltd. All rights reserved.

Assessment of Micro-Basin Tillage as a Soil and Water Conservation Practice in the Black Soil Region of Northeast China.

PubMed

Sui, Yuanyuan; Ou, Yang; Yan, Baixing; Xu, Xiaohong; Rousseau, Alain N; Zhang, Yu

2016-01-01

Micro-basin tillage is a soil and water conservation practice that requires building individual earth blocks along furrows. In this study, plot experiments were conducted to assess the efficiency of micro-basin tillage on sloping croplands between 2012 and 2013 (5°and 7°). The conceptual, optimal, block interval model was used to design micro-basins which are meant to capture the maximum amount of water per unit area. Results indicated that when compared to the up-down slope tillage, micro-basin tillage could increase soil water content and maize yield by about 45% and 17%, and reduce runoff, sediment and nutrients loads by about 63%, 96% and 86%, respectively. Meanwhile, micro-basin tillage could reduce the peak runoff rates and delay the initial runoff-yielding time. In addition, micro-basin tillage with the optimal block interval proved to be the best one among all treatments with different intervals. Compared with treatments of other block intervals, the optimal block interval treatments increased soil moisture by around 10% and reduced runoff rate by around 15%. In general, micro-basin tillage with optimal block interval represents an effective soil and water conservation practice for sloping farmland of the black soil region.

Assessment of Micro-Basin Tillage as a Soil and Water Conservation Practice in the Black Soil Region of Northeast China

PubMed Central

Sui, Yuanyuan; Ou, Yang; Yan, Baixing; Xu, Xiaohong; Rousseau, Alain N.; Zhang, Yu

2016-01-01

Micro-basin tillage is a soil and water conservation practice that requires building individual earth blocks along furrows. In this study, plot experiments were conducted to assess the efficiency of micro-basin tillage on sloping croplands between 2012 and 2013 (5°and 7°). The conceptual, optimal, block interval model was used to design micro-basins which are meant to capture the maximum amount of water per unit area. Results indicated that when compared to the up-down slope tillage, micro-basin tillage could increase soil water content and maize yield by about 45% and 17%, and reduce runoff, sediment and nutrients loads by about 63%, 96% and 86%, respectively. Meanwhile, micro-basin tillage could reduce the peak runoff rates and delay the initial runoff-yielding time. In addition, micro-basin tillage with the optimal block interval proved to be the best one among all treatments with different intervals. Compared with treatments of other block intervals, the optimal block interval treatments increased soil moisture by around 10% and reduced runoff rate by around 15%. In general, micro-basin tillage with optimal block interval represents an effective soil and water conservation practice for sloping farmland of the black soil region. PMID:27031339

The conductivity measurements applied for the evaluation of controlled release of chlorhexidine from thermosensitive N-isopropylacrylamide derivative microgels.

PubMed

Musiał, Witold; Kokol, Vanja; Voncina, Bojana

2009-01-01

The aim of the work was the evaluation of the conductivity changes in aqueous environment, consisting of chlorhexidine, and N-isopropylacrylamide derivative microgel, during increasing the temperature between 25 degrees C and 42 degrees C, as a prerequisite to develop the this microgel for controlled release of chlorhexidine, when alterations in temperature are involved. Conductivity of studied systems underwent specific alterations, when temperature increased. For the system with polymer PNM I the values of conductivity were in the range 104,47 microS/cm - 134,70 microS/ cm, for temperature range 25 degrees C and 42 degrees C. In the case of PNM II - CX system, respective values reached 91,75 microS/cm - 135,95 microS/cm. The lowest conductivity values were observed when PNM III - CX mixture was studied: 96,90 microS/cm and 117,37 microS/cm. When a complex of derivatives of N-isopropylacrylamide with chlorhexidine undergoes thermal alteration, there is a potential to obtain controlled release of chlorhexidine from the polymeric bead in the range between 25 degrees C and 42 degrees C. The affinity of chlorhexidine to the polymer may be assessed in this systems applying the conductivity measurements. The solubility of chlorhexidine in the polymeric systems should be in future evaluated, to determine role of this factor in the conductivity alterations.

Flow control of micro-ramps on supersonic forward-facing step flow

NASA Astrophysics Data System (ADS)

Qing-Hu, Zhang; Tao, Zhu; Shihe, Yi; Anping, Wu

2016-05-01

The effects of the micro-ramps on supersonic turbulent flow over a forward-facing step (FFS) was experimentally investigated in a supersonic low-noise wind tunnel at Mach number 3 using nano-tracer planar laser scattering (NPLS) and particle image velocimetry (PIV) techniques. High spatiotemporal resolution images and velocity fields of supersonic flow over the testing model were captured. The fine structures and their spatial evolutionary characteristics without and with the micro-ramps were revealed and compared. The large-scale structures generated by the micro-ramps can survive the downstream FFS flowfield. The micro-ramps control on the flow separation and the separation shock unsteadiness was investigated by PIV results. With the micro-ramps, the reduction in the range of the reversal flow zone in streamwise direction is 50% and the turbulence intensity is also reduced. Moreover, the reduction in the average separated region and in separation shock unsteadiness are 47% and 26%, respectively. The results indicate that the micro-ramps are effective in reducing the flow separation and the separation shock unsteadiness. Project supported by the National Natural Science Foundation of China (Grant Nos. 11172326 and 11502280).

Biomechanical aspects of initial intraosseous stability and implant design: a quantitative micro-morphometric analysis.

PubMed

Akça, Kivanç; Chang, Ting-Ling; Tekdemir, Ibrahim; Fanuscu, Mete I

2006-08-01

The objective of this biomechanical study was to explore the effect of bone micro-morphology on initial intraosseous stability of implants with different designs. Straumann and Astra Tech dental implants were placed into anterior and posterior regions of completely edentulous maxilla and mandible of a human cadaver. Experiments were undertaken to quantify initial implant stability and bone micro-morphology. Installation torque values (ITVs) and implant stability quotients (ISQs) were measured to determine initial intraosseous implant stability. For quantification of relative bone volume and micro-architecture, sectioned implant-bone and bone core specimens of each implant placement site were consecutively scanned and trabecular bone was analyzed in a micro-computed tomography (micro-CT) unit. Experimental outcomes were evaluated for correlations among implant designs, initial intraosseous implant stability and bone micro-structural parameters. ITVs correlated higher with bone volume fraction (BV/TV) than ISQs, at 88.1% and 68.9% levels, respectively. Correlations between ITVs and micro-morphometric parameters were significant at the 95% confidence level (P<0.05) while ISQs were not. Differences in ITVs, ISQs and BV/TV data in regards to implant designs used were not significant at the 95% confidence level (P>0.05). Bone micro-morphology has a prevailing effect over implant design on intraosseus initial implant stability, and ITV is more sensitive in terms of revealing biomechanical properties at the bone-implant interface in comparison with ISQ.

Development of feedback-speed-control system of fixed-abrasive tool for mat-surface fabrication

NASA Astrophysics Data System (ADS)

Yanagihara, K.; Kita, R.

2018-01-01

This study deals with the new method to fabricate a mat-surface by using fixed-abrasive tool. Mat-surface is a surface with microscopic irregularities whose dimensions are close to the wavelengths of visible light (400-700 nanometers). In order to develop the new method to fabricate mat-surface without pre-masking and large scale back up facility, utilization of fixed-abrasive tool is discussed. The discussion clarifies that abrasives in shot blasting are given kinetic energy along to only plunge-direction while excluding traverse-direction. If the relative motion between tool and work in fixed-abrasive process can be realized as that in blasting, mat-surface will be accomplished with fixed-abrasive process. To realize the proposed idea, new surface-fabrication system to which is adopted feedback-speed-control of abrasive wheel has been designed. The system consists of micro-computer unit (MPU), work-speed sensor, fixed-abrasive wheel, and wheel driving unit. The system can control relative speed between work and wheel in optimum range to produce mat-surface. Finally experiment to verify the developed system is carried out. The results of experiments show that the developed system is effective and it can produce the surface from grinding to mat-surface seamlessly.

Micro-satellites thermal control—concepts and components

NASA Astrophysics Data System (ADS)

Baturkin, Volodymyr

2005-01-01

The main idea of this paper is to present the survey of current tendencies in micro-satellites thermal control concepts that can be rational and useful for posterior missions due to intensive expansion of satellites of such type. For this purpose, the available references and lessons learned by the National Technical University of Ukraine during the elaboration of thermal control hardware for micro-satellites Magion 4, 5, BIRD and autonomous thermal control systems for interplanetary missions VEGA, PHOBOS have been used. The main parameters taken into consideration for analysis are the satellite sizes, mass, power consumption, orbit parameters, altitude control peculiarities and thermal control description. It was defined that passive thermal control concepts are widely used, excepting autonomous temperature regulation for sensitive components such as batteries, high-precision optics, and some types of sensors. The practical means for realization of passive thermal control design as multi-layer insulation, optical coatings, heat conductive elements, gaskets are briefly described.

MicroRNA-21 in laryngeal squamous cell carcinoma: Diagnostic and prognostic features.

PubMed

Erkul, Evren; Yilmaz, Ismail; Gungor, Atila; Kurt, Onuralp; Babayigit, Mustafa A

2017-02-01

We aimed to determine the microRNA-21 expression in laryngeal squamous cell carcinoma and assess the association between the disease and clinical characteristics of patients. Retrospective case-control study. A retrospective study was conducted from January 2005 to May 2011, in a tertiary hospital following tumor resection in 72 patients with laryngeal squamous cell carcinoma. We used formalin-fixed paraffin-embedded tissue samples of laryngeal squamous cell carcinomas (study group) and adjacent nontumor tissues (control group) for microRNA-21 expressions, and we successfully extracted microRNAs detectable by real-time polymerase chain reaction. All patients were evaluated separately, and the study and control groups were compared. The study group was assessed in terms of localization, smoking, alcohol consumption, lymph node staging, tumor stage, overall survival, disease-free survival, perineural, and vascular invasion. All patients were male, and the average age of patients was 64.2 ± 10.3 years. MicroRNA-21 was upregulated in laryngeal squamous cell carcinomas compared to adjacent nontumor tissues (P = .005). However, the microRNA-21 did not differ significantly according to any clinicopathological features (P > .05). MicroRNA-21 has been found to be expressed at lower levels in early stage (stages 1 and 2) compared with advanced stage (stages 3 and 4), but this was not statistically significant (P = .455). We conclude that the microRNA-21 level may play an important role in diagnosis and serve as a potential biomarker; such measurement thus has clinical applications. However, any possible prognostic associations with microRNA-21 levels should be re-evaluated in future studies on laryngeal squamous cell carcinoma samples amenable to retrospective analysis. NA Laryngoscope, 2016 127:E62-E66, 2017. © 2016 The American Laryngological, Rhinological and Otological Society, Inc.

The Patentability of Living Organisms under 35 U.S.C. Section 101: In re Bergy.

ERIC Educational Resources Information Center

Harvard Law Review, 1978

1978-01-01

Examined is the In re Bergy decision in which a divided United States Court of Customs and Patent Appeals held that a living micro-organism was patentable under section 101 of the United States Code. Differing views and problems with the decision are also discussed. (JMD)

Some results from studies on the effects of weightlessness on the growth of epiphytic orchids

NASA Technical Reports Server (NTRS)

Cherevchenko, T. M.; Mayko, T. K.

1983-01-01

Epidendrum orchids were placed in a Malakhit-2 micro-greenhouse aboard the Soyuz-36-Salyut-6 space station to test their growth under weightless conditions. Growth occurred but was less than in control plants left on Earth; cells were smaller and parenchymal development slowed in all tissues. Stems, roots, and leaves were smaller. The number of stomas on the leaves was about the same as in the controls, but, because of the smaller leaf size, there were more per unit area. A modeling experiment using a clinostat revealed a large decrease in gibberellin activity and auxin activity. It was assumed that weightlessness primarily affects gibberellin biosynthesis, inhibiting cell growth. Reestablishment of growth compound activity upon return of the plants to Earth was indicated by the fact that the orchids resumed growth thereafter.

Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement

DOE Office of Scientific and Technical Information (OSTI.GOV)

Johnson, Chelsea R.; Millwood, Reginald J.; Tang, Yuhong

Genetic engineering has been effective in altering cell walls for biofuel production in the bioenergy crop, switchgrass (Panicum virgatum). However, regulatory issues arising from gene flow may prevent commercialization of engineered switchgrass in the eastern United States where the species is native. And, depending on its expression level, microRNA156 (miR156) can reduce, delay, or eliminate flowering, which may serve to decrease transgene flow. Here, in this unique field study of transgenic switchgrass that was permitted to flower, two low (T14 and T35) and two medium (T27 and T37) miR156-overexpressing 'Alamo' lines with the transgene under the control of the constitutivemore » maize (Zea mays) ubiquitin 1 promoter, along with nontransgenic control plants, were grown in eastern Tennessee over two seasons.« less

Field-grown miR156 transgenic switchgrass reproduction, yield, global gene expression analysis, and bioconfinement

DOE PAGES

Johnson, Chelsea R.; Millwood, Reginald J.; Tang, Yuhong; ...

2017-11-30

Genetic engineering has been effective in altering cell walls for biofuel production in the bioenergy crop, switchgrass (Panicum virgatum). However, regulatory issues arising from gene flow may prevent commercialization of engineered switchgrass in the eastern United States where the species is native. And, depending on its expression level, microRNA156 (miR156) can reduce, delay, or eliminate flowering, which may serve to decrease transgene flow. Here, in this unique field study of transgenic switchgrass that was permitted to flower, two low (T14 and T35) and two medium (T27 and T37) miR156-overexpressing 'Alamo' lines with the transgene under the control of the constitutivemore » maize (Zea mays) ubiquitin 1 promoter, along with nontransgenic control plants, were grown in eastern Tennessee over two seasons.« less

High density pixel array

NASA Technical Reports Server (NTRS)

McFall, James Earl (Inventor); Wiener-Avnear, Eliezer (Inventor)

2004-01-01

A pixel array device is fabricated by a laser micro-milling method under strict process control conditions. The device has an array of pixels bonded together with an adhesive filling the grooves between adjacent pixels. The array is fabricated by moving a substrate relative to a laser beam of predetermined intensity at a controlled, constant velocity along a predetermined path defining a set of grooves between adjacent pixels so that a predetermined laser flux per unit area is applied to the material, and repeating the movement for a plurality of passes of the laser beam until the grooves are ablated to a desired depth. The substrate is of an ultrasonic transducer material in one example for fabrication of a 2D ultrasonic phase array transducer. A substrate of phosphor material is used to fabricate an X-ray focal plane array detector.

Integration of a MicroCAT Propulsion System and a PhoneSat Bus into a 1.5U CubeSat

NASA Technical Reports Server (NTRS)

Agasid, Elwood Floyd; Perez, Andres Dono; Gazulla, Oriol Tintore; Trinh, Greenfield Tran; Uribe, Eddie Anthony; Keidar, Michael; Haque, Samudra; Teel, George

2014-01-01

NASA Ames Research Center and the George Washington University have developed an electric propulsion subsystem that can be integrated into the PhoneSat bus. Experimental tests have shown a reliable performance by firing three different thrusters at various frequencies in vacuum conditions. The three thrusters were controlled by a SmartPhone that was running the PhoneSat software. The subsystem is fully operational and it requires low average power to function (about 0.1 W). The interface consists of a microcontroller that sends a trigger pulses to the PPU (Plasma Processing Unit), which is responsible for the thruster operation. Frequencies ranging from 1 to 50Hz have been tested, showing a strong flexibility. A SmartPhone acts as the main user interface for the selection of commands that control the entire system. The micro cathode arc thruster MicroCAT provides a high 1(sub sp) of 3000s that allows a 4kg satellite to obtain a (delta)V of 300m/s. The system mass is only 200g with a total of volume of 200(cu cm). The propellant is based on a solid cylinder made of Titanium, which is the cathode at the same time. This simplicity in the design avoids miniaturization and manufacturing problems. The characteristics of this thruster allow an array of MicroCATs to perform attitude control and orbital correcton maneuvers that will open the door for the implementation of an extensive collection of new mission concepts and space applications for CubeSats. NASA Ames is currently working on the integration of the system to fit the thrusters and PPU inside a 1.5U CubeSat together with the PhoneSat bus into a 1.5U CubeSat. This satellite is intended to be deployed from the ISS in 2015 and test the functionality of the thrusters by spinning the satellite around its long axis and measure the rotational speed with the phone byros. This test flight will raise the TRL of the propulsion system from 5 to 7 and will be a first test for further CubeSats with propulsion systems, a key subsystem for long duration or interplanetary CubeSat missions.

Optimal Control of Micro Grid Operation Mode Seamless Switching Based on Radau Allocation Method

NASA Astrophysics Data System (ADS)

Chen, Xiaomin; Wang, Gang

2017-05-01

The seamless switching process of micro grid operation mode directly affects the safety and stability of its operation. According to the switching process from island mode to grid-connected mode of micro grid, we establish a dynamic optimization model based on two grid-connected inverters. We use Radau allocation method to discretize the model, and use Newton iteration method to obtain the optimal solution. Finally, we implement the optimization mode in MATLAB and get the optimal control trajectory of the inverters.

Micro-domain controlled anisotropic laser ceramics assisted by rare-earth trivalent

NASA Astrophysics Data System (ADS)

Sato, Yoichi; Akiyama, Jun; Taira, Takunori

2012-01-01

Principles that enable to synthesize anisotropic laser ceramics have been established. Anisotropic laser ceramics contain micro domains made of anisotropic crystals, and we have invented the novel alignment technology of micro domain structure in laser ceramics assisted by rare-earth trivalent. Our novel process is essentially superior to the traditional electromagnetic processing from the viewpoint of mass production. We discussed the significance of anisotropic laser ceramics, and we also show the result of evaluations to our orientation controlled RE:FAP ceramics.

Application of Flexible Micro Temperature Sensor in Oxidative Steam Reforming by a Methanol Micro Reformer

PubMed Central

Lee, Chi-Yuan; Lee, Shuo-Jen; Shen, Chia-Chieh; Yeh, Chuin-Tih; Chang, Chi-Chung; Lo, Yi-Man

2011-01-01

Advances in fuel cell applications reflect the ability of reformers to produce hydrogen. This work presents a flexible micro temperature sensor that is fabricated based on micro-electro-mechanical systems (MEMS) technology and integrated into a flat micro methanol reformer to observe the conditions inside that reformer. The micro temperature sensor has higher accuracy and sensitivity than a conventionally adopted thermocouple. Despite various micro temperature sensor applications, integrated micro reformers are still relatively new. This work proposes a novel method for integrating micro methanol reformers and micro temperature sensors, subsequently increasing the methanol conversion rate and the hydrogen production rate by varying the fuel supply rate and the water/methanol ratio. Importantly, the proposed micro temperature sensor adequately controls the interior temperature during oxidative steam reforming of methanol (OSRM), with the relevant parameters optimized as well. PMID:22319407

Development of Laser Propulsion and Tracking System for Laser-Driven Micro-Airplane

NASA Astrophysics Data System (ADS)

Ishikawa, Hiroyasu; Kajiwara, Itsuro; Hoshino, Kentaro; Yabe, Takashi; Uchida, Shigeaki; Shimane, Yoshichika

2004-03-01

The purposes of this paper are to improve the control performance of the developed laser tracking system and to develop an integrated laser propulsion/tracking system for realizing a continuous flight and control of the micro-airplane. The laser propulsion is significantly effective to achieve the miniaturization and lightening of the micro-airplane. The laser-driven micro-airplane has been studied with a paper-craft airplane and YAG laser, resulting in a successful glide of the airplane. In the next stage of the laser-driven micro-airplane development, the laser tracking is expected as key technologies to achieve continuous propulsion. Furthermore, the laser propulsion system should be combined with the laser tracking system to supply continuous propulsion. Experiments are carried out to evaluate the performance of the developed laser tracking system and integrated laser propulsion/tracking system.

Prostate Cancer Metastases Alter Bone Mineral and Matrix Composition Independent of Effects on Bone Architecture in Mice A Quantitative Study Using microCT and Raman Spectroscopy

PubMed Central

Bi, Xiaohong; Sterling, Julie A.; Merkel, Alyssa R.; Perrien, Daniel S.; Nyman, Jeffry; Mahadevan-Jansen, Anita

2013-01-01

Prostate cancer is the most common primary tumor and the second leading cause of cancer-related deaths in men in the United States. Prostate cancer bone metastases are characterized by abnormal bone remodeling processes and result in a variety of skeletal morbidities. Prevention of skeletal complications is a crucial element in prostate cancer management. This study investigated prostate cancer-induced alterations in the molecular composition and morphological structure of metastasis-bearing bones in a mouse model of prostate cancer using Raman spectroscopy and micro-computed tomography (microCT). LNCaP C4-2B prostate cancer cells were injected into the right tibiae of 5-week old male SCID mice. Upon sacrifice at 8 weeks post tumor inoculation, two out of the ten tumor-bearing tibiae showed only osteoblastic lesions in the radiographs, 4 osteolytic lesions only and 4 mixed with osteoblastic and osteolytic lesions.. Carbonate substitution was significantly increased while there was a marked reduction in the level of collagen mineralization, mineral crystallinity, and carbonate:matrix ratio in the cortex of the intact tumor-bearing tibiae compared to contralateral controls. MicroCT analysis revealed a significant reduction in bone volume/total volume, trabecular number and trabecular thickness, as well as significant increase in bone surface/volume ratio in tibiae with osteolytic lesions, suggesting active bone remodeling and bone loss. None of the changes in bone compositional properties were correlated with lesion area from radiographs or the changes in bone architecture from microCT. This study indicates that LNCaP C4-2B prostate cancer metastases alter bone tissue composition independent of changes in architecture, and altered bone quality may be an important contributor to fracture risk in these patients. Raman spectroscopy may provide a new avenue of investigation into interactions between tumor and bone microenvironment. PMID:23867219

Exploring alternative Zn-binding groups in the design of HDAC inhibitors: squaric acid, N-hydroxyurea, and oxazoline analogues of SAHA.

PubMed

Hanessian, Stephen; Vinci, Valerio; Auzzas, Luciana; Marzi, Mauro; Giannini, Giuseppe

2006-09-15

Analogues of suberoylanilide hydroxamic acid (SAHA) were prepared by replacing the Zn-binding group with squaric acid, N-hydroxyurea, and 4-hydroxymethyl oxazoline units, also varying the length of the aliphatic chain. No inhibitory activity on HDAC was observed below 1.0 microM and no cytotoxic activity on different tumor cell lines was seen below 20.0 microM.

Adrenergic and cholinergic activity contributes to the cardiovascular effects of lionfish (Pterois volitans) venom.

PubMed

Church, Jarrod E; Hodgson, Wayne C

2002-06-01

The aim of the present study was to further investigate the cardiovascular activity of Pterois volitans crude venom. Venom (0.6-18 microg protein/ml) produced dose- and endothelium-dependent relaxation in porcine coronary arteries that was potentiated by atropine (10nM), but significantly attenuated by the nitric oxide synthase inhibitor N(omega)-nitro-L-arginine (NOLA; 0.1mM), by prior exposure of the tissue to stonefish antivenom (SFAV, 3 units/ml, 10 min), or by removal of extracellular Ca(2+). In rat paced left atria, venom (10 microg protein/ml) produced a decrease, followed by an increase, in contractile force. Atropine (0.5 microM) abolished the decrease in force and potentiated the increase. Propranolol (5 microM) did not affect the decrease in force but significantly attenuated the increase. In spontaneously beating right atria, venom (10 microg protein/ml) produced an increase in rate that was significantly attenuated by propranolol (5 microM). Prior incubation with SFAV (0.3 units/microg protein, 10 min) abolished both the inotropic and chronotropic responses to venom. In the anaesthetised rat, venom (100 micro protein/kg, i.v.) produced a pressor response, followed by a sustained depressor response. Atropine (1mg/kg, i.v.) potentiated the pressor response. The further addition of prazosin (50 microg/kg, i.v.) restored the original response to venom. Prior administration of SFAV (100 units/kg, i.v., 10 min) significantly attenuated the in vivo response to venom. It is concluded that P. volitans venom produces its cardiovascular effects primarily by acting on muscarinic cholinergic receptors and adrenoceptors. As SFAV neutralised many of the effects of P. volitans venom, we suggest that the two venoms share a similar component(s). Copright 2002 Elsevier Science Ltd.

Controlled banked turns in coleopteran flight measured by a miniature wireless inertial measurement unit.

PubMed

Li, Yao; Cao, Feng; Thang Vo Doan, Tat; Sato, Hirotaka

2016-09-28

The mechanisms and principles of insect flight have long been investigated by researchers working on micro and nano air vehicles (MAVs/NAVs). However, studies of insect flight maneuvers require high speed filming and high spatial resolution in a small experimental space, or the tethering of the insect to a fixed place. Under such artificial conditions, the insects may deviate its flying behavior from that of regular flight. In this study, we mounted a tiny wireless system, or 'backpack', on live beetles (Mecynorrhina torquata; length 62 ± 8 mm; mass 7.4 ± 1.3 g) freely flying in a large laboratory space. The backpack contains a micro inertial measurement unit (IMU) that was especially designed and manufactured for this purpose. Owing to the small mass (∼1.30 g) and dimensions (∼2.3 cm 2 ) of the backpack and the high accuracy of the IMU, we could remotely record the beetle in free flight. The free flight data revealed a strong linear correlation between the roll angle and yaw angular velocity. The strength of the correlation was quantified by the correlation coefficients and mean values. The change in roll angle preceded the change in yaw angular velocity. Moreover, there were frequent fluctuations in the roll angular velocity, which were uncorrelated with the yaw angular velocity. Apart from the strong correlation, these findings imply that Mecynorrhina torquata actively manipulates its roll rotation without coupling to the yaw rotation.

Technology Challenges in Solid Energetic Materials for Micro Propulsion Applications

DTIC Science & Technology

2009-11-01

thruster is a relatively new class of micro propulsion system for micro spacecraft , though there are many other potential uses in power generation...micro spacecraft , micro satellites (10 to 100 kg), nano satellites (1 to 10 kg), and pico satellites (0.1 to 1 kg). These small-scale satellites will...rocket thruster, assuming that it is used for the attitude control of a 10 kg spacecraft with 1 m/s velocity increment to maneuver around an object in

Further Development of an Exhaled microRNA Biomarker of Lung Cancer Risk

DTIC Science & Technology

2017-08-01

devise a non -invasive airway based exhaled microRNA metric for lung cancer risk, initial work to be tested in a case control study. We expanded the...control study. We also expanded the assessment of lung cancer subjects to also include limited (3-month) prospective follow-up of non -cancer controls...follow-up of non -cancer controls in order to minimize control contamination/misclassification, and improved matching on age, smoking status, and

PLZT Ceramic Driving Rotary Micro-mirror Based on Photoelectric-electrostatic Mechanism

NASA Astrophysics Data System (ADS)

Tang, Yujuan; Yang, Zhong; Chen, Yusong; Wang, Xinjie

2017-12-01

Based on the anomalous photovoltaic effect of PLZT, a rotary micro-mirror driven by hybrid photoelectric-electrostatic actuation of PLZT ceramic is proposed. Firstly, the mathematical modelling of coupled multi-physics fields of PLZT ceramic is established during illumination and light off phases. Then, the relationship between the rotation angle and the photovoltage of PLZT ceramics is established. In addition, the feasibility of rotary micro-mirror with hybrid photoelectric-electrostatic driving is verified via closed-loop control for photo-induced voltage of PLZT ceramic. The experimental results show that the photo-induced voltage of PLZT ceramics has good dynamic control precision using on-off closed-loop control method.

A price mechanism for supply demand matching in local grid of households with micro-CHP

NASA Astrophysics Data System (ADS)

Larsen, G. K. H.; van Foreest, N. D.; Scherpen, J. M. A.

2012-10-01

This paper describes a dynamic price mechanism to coordinate eletric power generation from micro Combined Heat and Power (micro-CHP) systems in a network of households. It is assumed that the households are prosumers, i.e. both producers and consumers of electricity. The control is done on household level in a completely distributed manner. Avoiding a centralized controller both eases computation complexity and preserves communication structure in the network. Local information is used to decide to turn on or off the micro-CHP, but through price signals between the prosumers the network as a whole operates in a cooperative way.

Patterned graphene functionalization via mask-free scanning of micro-plasma jet under ambient condition

NASA Astrophysics Data System (ADS)

Ye, Dong; Wu, Shu-Qun; Yu, Yao; Liu, Lin; Lu, Xin-Pei; Wu, Yue

2014-03-01

In this work, a mask-free method is introduced for patterned nitrogen doping of graphene using a micro-plasma jet under ambient condition. Raman and X-ray photoelectron spectroscopy spectra indicate that nitrogen atoms are incorporated into the graphene lattice with the two-dimensional spatial distribution precisely controlled in the range of mm down to 10 μm. Since the chemistry of the micro-plasma jet can be controlled by the choice of the gas mixture, this direct writing process with micro-plasma jet can be a versatile approach for patterned functionalization of graphene with high spatial resolution. This could have promising applications in graphene-based electronics.

MicroRNA Expression Profile in the Prenatal Amniotic Fluid Samples of Pregnant Women with Down Syndrome.

PubMed

Karaca, Emin; Aykut, Ayça; Ertürk, Biray; Durmaz, Burak; Güler, Ahmet; Büke, Barış; Yeniel, Ahmet Özgür; Ergenoğlu, Ahmet Mete; Özkınay, Ferda; Özeren, Mehmet; Kazandı, Mert; Akercan, Fuat; Sağol, Sermet; Gündüz, Cumhur; Çoğulu, Özgür

2018-03-15

Down syndrome, which is the most common human chromosomal anomaly that can affect people of any race and age, can be diagnosed prenatally in most cases. Prenatal diagnosis via culture method is time-consuming; thus, genetic analysis has thus been introduced and is continually being developed for rapid prenatal diagnosis. For this reason, the effective use of microRNA profiling for the rapid analysis of prenatal amniotic fluid samples for the diagnosis of Down syndrome was investigated. To evaluate the expression levels of 14 microRNAs encoded by chromosome 21 in amniotic fluid samples and their utility for prenatal diagnosis of Down syndrome. Case-control study. We performed invasive prenatal testing for 56 pregnant women; 23 carried fetuses with Down syndrome, and 33 carried fetuses with a normal karyotype. Advanced maternal age and increased risk for Down syndrome in the screening tests were indications for invasive prenatal testing. The age of gestation in the study and control groups ranged between 17 and 18 weeks. The expression levels of microRNA were measured by real-time polymerase chain reaction. The expression levels of microRNA-125b-2, microRNA-155 , and microRNA-3156 were significantly higher in the study group than in the control group. The presence of significantly dysregulated microRNAs may be associated with either the phenotype or the result of abnormal development. Further large-scale comparative studies conducted in a variety of conditions may bring novel insights in the field of abnormal prenatal conditions.

On Study of Application of Micro-reactor in Chemistry and Chemical Field

NASA Astrophysics Data System (ADS)

Zhang, Yunshen

2018-02-01

Serving as a micro-scale chemical reaction system, micro-reactor is characterized by high heat transfer efficiency and mass transfer, strictly controlled reaction time and good safety performance; compared with the traditional mixing reactor, it can effectively shorten reaction time by virtue of these advantages and greatly enhance the chemical reaction conversion rate. However, problems still exist in the process where micro-reactor is used for production in chemistry and chemical field, and relevant researchers are required to optimize and perfect the performance of micro-reactor. This paper analyzes specific application of micro-reactor in chemistry and chemical field.

Subacute toxicity of nano-selenium compared to other selenium species in mice.

PubMed

Benko, Ilona; Nagy, Gabor; Tanczos, Bence; Ungvari, Eva; Sztrik, Attila; Eszenyi, Peter; Prokisch, Jozsef; Banfalvi, Gaspar

2012-12-01

Sixteen groups of mice were fed diets containing different selenium species to compare their toxicity. Inorganic sodium selenate and sodium hydroselenite, elementary nanoSe, organic Sel-Plex, and Lacto-MicroSelenium were administered for 14 d at concentrations of 0.5, 5, and 50 ppm Se, equivalent to 0.5, 5, and 50 mg Se/kg food, corresponding to an estimated 4, 40, and 400 µg/kg body weight/d Se uptake, respectively. At the end of the treatment, body, liver, spleen, kidney, heart, and brain weights were measured, mice were subjected to necropsy, and histological examinations were performed on the liver. At lower Se doses (0.5 and 5 ppm) a moderate reduction was observed in the number of bone marrow and white blood cells and in granulocyte-macrophage colony-forming units (GM-CFUs) relative to the untreated control group of mice. A comparison of lowest toxic doses of sodium selenite in mice (0.5 ppm) and mallard (10 ppm) indicates that birds are more resistant to Se than rodents. In mice, a small but measurable weight loss was observed after 5 ppm selenate and LactoMicroSe treatment. The most significant changes took place after 50-ppm administration in body and spleen weight, hematology, and liver histology. Toxicity was more pronounced when inorganic Se was applied than after subacute application of Sel-Plex, nanoSe, or LactoMicroSe. To summarize the effects, the authors' 14-d murine subacute toxicity study showed that the toxicity of Se species decreased in the following order: selenate > selenite > nanoSe > Sel-Plex > LactoMicroSe. Copyright © 2012 SETAC.

Cement Thickness of Inlay Restorations Made of Lithium Disilicate, Polymer-Infiltrated Ceramic and Nano-Ceramic CAD/CAM Materials Evaluated Using 3D X-Ray Micro-Computed Tomography.

PubMed

Uzgur, Recep; Ercan, Ertuğrul; Uzgur, Zeynep; Çolak, Hakan; Yalçın, Muhammet; Özcan, Mutlu

2016-08-12

To evaluate the marginal and internal cement thicknesses of inlay restorations made of various CAD/CAM materials using 3D X-ray micro-computed tomography (micro-CT) technique. Caries-free extracted mandibular molars (N = 30) with similar size were randomly assigned to three groups (N = 10 per group). Mesio-occlusal-distal (MOD) cavities were prepared, and inlay restorations were obtained by milling out CAD/CAM materials namely, (a) IPS: monolithic lithium disilicate (control), (b) VE: polymer-infiltrated ceramic, and (c) CS: nano-ceramic using a CAM unit. Marginal and internal cement thicknesses were measured using 3D micro-CT. Data were analyzed using 1-way ANOVA and Tukey's tests (alpha = 0.05). The mean marginal and internal cement thickness were not significant in all inlay materials (p > 0.05). Mean marginal cement thickness (μm) was the lowest for the IPS group (67.54 ± 10.16) followed by VE (84.09 ± 3.94) and CS (95.18 ± 10.58) (p > 0.05). The internal cement thickness (μm) was the lowest in the CS group (54.85 ± 6.94) followed by IPS (60.58 ± 9.22) and VE (77.53 ± 12.13) (p > 0.05). Marginal and internal cement thicknesses of MOD inlays made of monolithic lithium disilicate, polymer-infiltrated ceramic, and nano-ceramic CAD/CAM materials were similar and all less than 100 μm, which could be considered clinically acceptable. MOD inlays made of different CAD/CAM materials presented similar cement thickness, less than 100 μm. © 2016 by the American College of Prosthodontists.

Design of PID temperature control system based on STM32

NASA Astrophysics Data System (ADS)

Zhang, Jianxin; Li, Hailin; Ma, Kai; Xue, Liang; Han, Bianhua; Dong, Yuemeng; Tan, Yue; Gu, Chengru

2018-03-01

A rapid and high-accuracy temperature control system was designed using proportional-integral-derivative (PID) control algorithm with STM32 as micro-controller unit (MCU). The temperature control system can be applied in the fields which have high requirements on the response speed and accuracy of temperature control. The temperature acquisition circuit in system adopted Pt1000 resistance thermometer as temperature sensor. Through this acquisition circuit, the monitoring actual temperature signal could be converted into voltage signal and transmitted into MCU. A TLP521-1 photoelectric coupler was matched with BD237 power transistor to drive the thermoelectric cooler (TEC) in FTA951 module. The effective electric power of TEC was controlled by the pulse width modulation (PWM) signals which generated by MCU. The PWM signal parameters could be adjusted timely by PID algorithm according to the difference between monitoring actual temperature and set temperature. The upper computer was used to input the set temperature and monitor the system running state via serial port. The application experiment results show that the temperature control system is featured by simple structure, rapid response speed, good stability and high temperature control accuracy with the error less than ±0.5°C.

A miniature ultrasonic actuator-control system for plant stem diameter micro-variation measurements

USDA-ARS?s Scientific Manuscript database

Measurements of micro-variations in plant stem diameter are potentially useful to optimize irrigation decision support systems that are based on plant physiological responses. However, for this technology to be suitable for field applications, problems associated with stem softness and micro variati...

78 FR 25940 - Submission for OMB Review; Comment Request

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-03

... Service Title: Rural Micro-Entrepreneur Assistance Program. OMB Control Number: 0570-0062. Summary of... Rural Micro- entrepreneur Assistance Program (RMAP). The Secretary makes direct loans to rural... rate business loans of $50,000 or less to micro- entrepreneurs, as defined in the 2008 Farm Bill. Need...

Modulation of the release of ( sup 3 H)norepinephrine from the base and body of the rat urinary bladder by endogenous adrenergic and cholinergic mechanisms

DOE Office of Scientific and Technical Information (OSTI.GOV)

Somogyi, G.T.; de Groat, W.C.

Modulation of (3H)NE release was studied in rat urinary bladder strips prelabeled with (3H)NE. (3H)NE uptake occurred in strips from the bladder base and body, but was very prominent in the base where the noradrenergic innervation is most dense. Electrical field stimulation markedly increased (3H)NE outflow from the superfused tissue. The quantity of (3H)NE release was approximately equal during three consecutive periods of stimulation. Activation of presynaptic muscarinic receptors by 1.0 microM oxotremorine reduced (3H)NE release to 46% of the control. Atropine (1 microM) blocked the effect of oxotremorine and increased the release to 147% of predrug control levels. Activationmore » of presynaptic alpha-2 adrenoceptors by 1 microM clonidine reduced (3H)NE release to 55% of control. Yohimbine blocked the action of clonidine and increased the release to 148% of control. The release of (3H)NE from the bladder base and body was increased by both 1 microM atropine (to 167% and 174% of control, respectively) and 1 microM yohimbine (to 286% and 425% of control, respectively). Atropine and yohimbine administered in combination had similar facilitatory effects as when administered alone. We conclude that the release of (3H)NE from adrenergic nerve endings in electrically stimulated bladder strips is modulated via endogenous transmitters acting on both muscarinic and alpha-2 adrenergic presynaptic receptors and that the latter provide the most prominent control.« less

Profile of cerebrospinal microRNAs in fibromyalgia.

PubMed

Bjersing, Jan L; Lundborg, Christopher; Bokarewa, Maria I; Mannerkorpi, Kaisa

2013-01-01

Fibromyalgia (FM) is characterized by chronic pain and reduced pain threshold. The pathophysiology involves disturbed neuroendocrine function, including impaired function of the growth hormone/insulin-like growth factor-1 axis. Recently, microRNAs have been shown to be important regulatory factors in a number of diseases. The aim of this study was to try to identify cerebrospinal microRNAs with expression specific for FM and to determine their correlation to pain and fatigue. The genome-wide profile of microRNAs in cerebrospinal fluid was assessed in ten women with FM and eight healthy controls using real-time quantitative PCR. Pain thresholds were examined by algometry. Levels of pain (FIQ pain) were rated on a 0-100 mm scale (fibromyalgia impact questionnaire, FIQ). Levels of fatigue (FIQ fatigue) were rated on a 0-100 mm scale using FIQ and by multidimensional fatigue inventory (MFI-20) general fatigue (MFIGF). Expression levels of nine microRNAs were significantly lower in patients with FM patients compared to healthy controls. The microRNAs identified were miR-21-5p, miR-145-5p, miR-29a-3p, miR-99b-5p, miR-125b-5p, miR-23a-3p, 23b-3p, miR-195-5p, miR-223-3p. The identified microRNAs with significantly lower expression in FM were assessed with regard to pain and fatigue. miR-145-5p correlated positively with FIQ pain (r=0.709, p=0.022, n=10) and with FIQ fatigue (r=0.687, p=0.028, n=10). To our knowledge, this is the first study to show a disease-specific pattern of cerebrospinal microRNAs in FM. We have identified nine microRNAs in cerebrospinal fluid that differed between FM patients and healthy controls. One of the identified microRNAs, miR-145 was associated with the cardinal symptoms of FM, pain and fatigue.

The performance of residential micro-cogeneration coupled with thermal and electrical storage

NASA Astrophysics Data System (ADS)

Kopf, John

Over 80% of residential secondary energy consumption in Canada and Ontario is used for space and water heating. The peak electricity demands resulting from residential energy consumption increase the reliance on fossil-fuel generation stations. Distributed energy resources can help to decrease the reliance on central generation stations. Presently, distributed energy resources such as solar photovoltaic, wind and bio-mass generation are subsidized in Ontario. Micro-cogeneration is an emerging technology that can be implemented as a distributed energy resource within residential or commercial buildings. Micro-cogeneration has the potential to reduce a building's energy consumption by simultaneously generating thermal and electrical power on-site. The coupling of a micro-cogeneration device with electrical storage can improve the system's ability to reduce peak electricity demands. The performance potential of micro-cogeneration devices has yet to be fully realized. This research addresses the performance of a residential micro-cogeneration device and it's ability to meet peak occupant electrical loads when coupled with electrical storage. An integrated building energy model was developed of a residential micro-cogeneration system: the house, the micro-cogeneration device, all balance of plant and space heating components, a thermal storage device, an electrical storage device, as well as the occupant electrical and hot water demands. This model simulated the performance of a micro-cogeneration device coupled to an electrical storage system within a Canadian household. A customized controller was created in ESP-r to examine the impact of various system control strategies. The economic performance of the system was assessed from the perspective of a local energy distribution company and an end-user under hypothetical electricity export purchase price scenarios. It was found that with certain control strategies the micro-cogeneration system was able to improve the economic performance for both the end user and local distribution company.

Emerging roles of microRNAs as molecular switches in the integrated circuit of the cancer cell

PubMed Central

Sotiropoulou, Georgia; Pampalakis, Georgios; Lianidou, Evi; Mourelatos, Zissimos

2009-01-01

Transformation of normal cells into malignant tumors requires the acquisition of six hallmark traits, e.g., self-sufficiency in growth signals, insensitivity to antigrowth signals and self-renewal, evasion of apoptosis, limitless replication potential, angiogenesis, invasion, and metastasis, which are common to all cancers (Hanahan and Weinberg 2000). These new cellular traits evolve from defects in major regulatory microcircuits that are fundamental for normal homeostasis. The discovery of microRNAs (miRNAs) as a new class of small non-protein-coding RNAs that control gene expression post-transcriptionally by binding to various mRNA targets suggests that these tiny RNA molecules likely act as molecular switches in the extensive regulatory web that involves thousands of transcripts. Most importantly, accumulating evidence suggests that numerous microRNAs are aberrantly expressed in human cancers. In this review, we discuss the emergent roles of microRNAs as switches that function to turn on/off known cellular microcircuits. We outline recent compelling evidence that deregulated microRNA-mediated control of cellular microcircuits cooperates with other well-established regulatory mechanisms to confer the hallmark traits of the cancer cell. Furthermore, these exciting insights into aberrant microRNA control in cancer-associated circuits may be exploited for cancer therapies that will target deregulated miRNA switches. PMID:19561119

Controllable Si (100) micro/nanostructures by chemical-etching-assisted femtosecond laser single-pulse irradiation

NASA Astrophysics Data System (ADS)

Li, Xiaowei; Xie, Qian; Jiang, Lan; Han, Weina; Wang, Qingsong; Wang, Andong; Hu, Jie; Lu, Yongfeng

2017-05-01

In this study, silicon micro/nanostructures of controlled size and shape are fabricated by chemical-etching-assisted femtosecond laser single-pulse irradiation, which is a flexible, high-throughput method. The pulse fluence is altered to create various laser printing patterns for the etching mask, resulting in the sequential evolution of three distinct surface micro/nanostructures, namely, ring-like microstructures, flat-top pillar microstructures, and spike nanostructures. The characterized diameter of micro/nanostructures reveals that they can be flexibly tuned from the micrometer (˜2 μm) to nanometer (˜313 nm) scales by varying the laser pulse fluence in a wide range. Micro-Raman spectroscopy and transmission electron microscopy are utilized to demonstrate that the phase state changes from single-crystalline silicon (c-Si) to amorphous silicon (a-Si) after single-pulse femtosecond laser irradiation. This amorphous layer with a lower etching rate then acts as a mask in the wet etching process. Meanwhile, the on-the-fly punching technique enables the efficient fabrication of large-area patterned surfaces on the centimeter scale. This study presents a highly efficient method of controllably manufacturing silicon micro/nanostructures with different single-pulse patterns, which has promising applications in the photonic, solar cell, and sensors fields.

Negative effects of microplastic exposure on growth and development of Crepidula onyx.

PubMed

Lo, Hau Kwan Abby; Chan, Kit Yu Karen

2018-02-01

Microplastics exposure could be detrimental to marine organisms especially under high concentrations. However, few studies have considered the multiphasic nature of marine invertebrates' life history and investigated the impact of experiencing microplastics during early development on post-metamorphic stages (legacy effect). Many planktonic larvae can feed selectively and it is unclear whether such selectivity could modulate the impact of algal food-sized microplastic. In this two-stage experiment, veligers of Crepidula onyx were first exposed to additions of algae-sized micro-polystyrene (micro-PS) beads at different concentrations, including ones that were comparable their algal diet. These additions were then either halted or continued after settlement. At environmentally relevant concentration (ten 2-μm microplastic beads ml -1 ), larval and juvenile C. onyx was not affected. At higher concentrations, these micro-PS fed larvae consumed a similar amount of algae compared to those in control but grew relatively slower than those in the control suggesting that ingestion and/or removal of microplastic was/were energetically costly. These larvae also settled earlier at a smaller size compared to the control, which could negatively affect post-settlement success. Juvenile C. onyx receiving continuous micro-PS addition had slower growth rates. Individuals only exposed to micro-PS during their larval stage continued to have slower growth rates than those in the control even if micro-PS had been absent in their surroundings for 65 days highlighting a legacy effect of microplastic exposure. Copyright © 2017 Elsevier Ltd. All rights reserved.

Effectiveness and micro-costing of the KiVa school-based bullying prevention programme in Wales: study protocol for a pragmatic definitive parallel group cluster randomised controlled trial.

PubMed

Clarkson, Suzy; Axford, Nick; Berry, Vashti; Edwards, Rhiannon Tudor; Bjornstad, Gretchen; Wrigley, Zoe; Charles, Joanna; Hoare, Zoe; Ukoumunne, Obioha C; Matthews, Justin; Hutchings, Judy

2016-02-01

Bullying refers to verbal, physical or psychological aggression repeated over time that is intended to cause harm or distress to the victims who are unable to defend themselves. It is a key public health priority owing to its widespread prevalence in schools and harmful short- and long-term effects on victims' well-being. There is a need to strengthen the evidence base by testing innovative approaches to preventing bullying. KiVa is a school-based bullying prevention programme with universal and indicated elements and an emphasis on changing bystander behaviour. It achieved promising results in a large trial in Finland, and now requires testing in other countries. This paper describes the protocol for a cluster randomised controlled trial (RCT) of KiVa in Wales. The study uses a two-arm waitlist control pragmatic definitive parallel group cluster RCT design with an embedded process evaluation and calculation of unit cost. Participating schools will be randomised a using a 1:1 ratio to KiVa plus usual provision (intervention group) or usual provision only (control group). The trial has one primary outcome, child self-reported victimisation from bullying, dichotomised as 'victimised' (bullied at least twice a month in the last couple of months) versus 'not victimised'. Secondary outcomes are: bullying perpetration; aspects of child social and emotional well-being (including emotional problems, conduct, peer relations, prosocial behaviour); and school attendance. Follow-up is at 12 months post-baseline. Implementation fidelity is measured through teacher-completed lesson records and independent school-wide observation. A micro-costing analysis will determine the costs of implementing KiVa, including recurrent and non-recurrent unit costs. Factors related to the scalability of the programme will be examined in interviews with head teachers and focus groups with key stakeholders in the implementation of school-based bullying interventions. The results from this trial will provide evidence on whether the KiVa programme is transportable from Finland to Wales in terms of effectiveness and implementation. It will provide information about the costs of delivery and generate insights into factors related to the scalability of the programme. Current Controlled Trials ISRCTN23999021 Date 10-6-13.

Macro-/Micro-Controlled 3D Lithium-Ion Batteries via Additive Manufacturing and Electric Field Processing.

PubMed

Li, Jie; Liang, Xinhua; Liou, Frank; Park, Jonghyun

2018-01-30

This paper presents a new concept for making battery electrodes that can simultaneously control macro-/micro-structures and help address current energy storage technology gaps and future energy storage requirements. Modern batteries are fabricated in the form of laminated structures that are composed of randomly mixed constituent materials. This randomness in conventional methods can provide a possibility of developing new breakthrough processing techniques to build well-organized structures that can improve battery performance. In the proposed processing, an electric field (EF) controls the microstructures of manganese-based electrodes, while additive manufacturing controls macro-3D structures and the integration of both scales. The synergistic control of micro-/macro-structures is a novel concept in energy material processing that has considerable potential for providing unprecedented control of electrode structures, thereby enhancing performance. Electrochemical tests have shown that these new electrodes exhibit superior performance in their specific capacity, areal capacity, and life cycle.

Trichoderma asperellum strain T34 controls Fusarium wilt disease in tomato plants in soilless culture through competition for iron.

PubMed

Segarra, Guillem; Casanova, Eva; Avilés, Manuel; Trillas, Isabel

2010-01-01

Trichoderma asperellum strain T34 has been reported to control the disease caused by Fusarium oxysporum f.sp. lycopersici (Fol) on tomato plants. To study the importance of iron concentration in the growth media for the activity and competitiveness of T34 and the pathogen, we tested four iron concentrations in the nutrient solution [1, 10, 100, and 1000 microM provided as EDTA/Fe(III)] in a biological control experiment with T34 and Fol in tomato plants. The reduction of the Fusarium-infected shoot by T34 was only significant at 10 microM Fe. We hypothesized that Fe competition is one of the key factors in the biocontrol activity exerted by T34 against Fol, as an increase in Fe concentration over 10 microM would lead to the suppression of T34 siderophore synthesis and thus inhibition of Fe competition with Fol. T34 significantly reduced the populations of Fol at all the doses of Fe assayed. In contrast, Fol enhanced the populations of T34 at 1 and 10 microM Fe. Nevertheless, several plant physiological parameters like net CO(2) assimilation (A), stomatal conductance (g(s)), relative quantum efficiency of PSII (Phi(PSII)), and efficiency of excitation energy capture by open PSII reactive centers (Fv'/Fm') demonstrated the protection against Fol damage by treatment with T34 at 100 microM Fe. The first physiological parameter affected by the disease progression was g(s). Plant dry weight was decreased by Fe toxicity at 100 and 1,000 microM. T34-treated plants had significantly greater heights and dry weights than control plants at 1,000 microM Fe, even though T34 did not reduce the Fe content in leaves or stems. Furthermore, T34 enhanced plant height even at the optimal Fe concentration (10 microM) compared to control plants. In conclusion, T. asperellum strain T34 protected tomato plants from both biotic (Fusarium wilt disease) and abiotic stress [Fe(III) toxic effects].

Dietary boron does not affect tooth strength, micro-hardness, and density, but affects tooth mineral composition and alveolar bone mineral density in rabbits fed a high-energy diet.

PubMed

Hakki, Sema S; SiddikMalkoc; Dundar, Niyazi; Kayis, Seyit Ali; Hakki, Erdogan E; Hamurcu, Mehmet; Baspinar, Nuri; Basoglu, Abdullah; Nielsen, Forrest H; Götz, Werner

2015-01-01

The objective of this study was to determine whether dietary boron (B) affects the strength, density and mineral composition of teeth and mineral density of alveolar bone in rabbits with apparent obesity induced by a high-energy diet. Sixty female, 8-month-old, New Zealand rabbits were randomly assigned for 7 months into five groups as follows: (1) control 1, fed alfalfa hay only (5.91 MJ/kg and 57.5 mg B/kg); (2) control 2, high energy diet (11.76 MJ and 3.88 mg B/kg); (3) B10, high energy diet + 10 mg B gavage/kg body weight/96 h; (4) B30, high energy diet + 30 mg B gavage/kg body weight/96 h; (5) B50, high energy diet + 50 mg B gavage/kg body weight/96 h. Maxillary incisor teeth of the rabbits were evaluated for compression strength, mineral composition, and micro-hardness. Enamel, dentin, cementum and pulp tissue were examined histologically. Mineral densities of the incisor teeth and surrounding alveolar bone were determined by using micro-CT. When compared to controls, the different boron treatments did not significantly affect compression strength, and micro-hardness of the teeth, although the B content of teeth increased in a dose-dependent manner. Compared to control 1, B50 teeth had decreased phosphorus (P) concentrations. Histological examination revealed that teeth structure (shape and thickness of the enamel, dentin, cementum and pulp) was similar in the B-treated and control rabbits. Micro CT evaluation revealed greater alveolar bone mineral density in B10 and B30 groups than in controls. Alveolar bone density of the B50 group was not different than the controls. Although the B treatments did not affect teeth structure, strength, mineral density and micro-hardness, increasing B intake altered the mineral composition of teeth, and, in moderate amounts, had beneficial effects on surrounding alveolar bone.

Micro-Organ Device

NASA Technical Reports Server (NTRS)

Sun, Wei (Inventor); Chang, Robert C. (Inventor); Starly, Binil (Inventor); Holtorf, Heidi L. (Inventor); Leslie, Julia (Inventor); Culbertson, Christopher (Inventor); Gonda, Steve R. (Inventor)

2013-01-01

A method for fabricating a micro-organ device comprises providing a microscale support having one or more microfluidic channels and one or more micro-chambers for housing a micro-organ and printing a micro-organ on the microscale support using a cell suspension in a syringe controlled by a computer-aided tissue engineering system, wherein the cell suspension comprises cells suspended in a solution containing a material that functions as a three-dimensional scaffold. The printing is performed with the computer-aided tissue engineering system according to a particular pattern. The micro-organ device comprises at least one micro-chamber each housing a micro-organ; and at least one microfluidic channel connected to the micro-chamber, wherein the micro-organ comprises cells arranged in a configuration that includes microscale spacing between portions of the cells to facilitate diffusion exchange between the cells and a medium supplied from the at least one microfluidic channel.

Micro-organ device

NASA Technical Reports Server (NTRS)

von Gustedt-Gonda, legal representative, Iris (Inventor); Holtorf, Heidi L. (Inventor); Gonda, Steve R. (Inventor); Leslie, Julia (Inventor); Chang, Robert C. (Inventor); Sun, Wei (Inventor); Starly, Binil (Inventor); Culbertson, Christopher (Inventor)

2013-01-01

A method for fabricating a micro-organ device comprises providing a microscale support having one or more microfluidic channels and one or more micro-chambers for housing a micro-organ and printing a micro-organ on the microscale support using a cell suspension in a syringe controlled by a computer-aided tissue engineering system, wherein the cell suspension comprises cells suspended in a solution containing a material that functions as a three-dimensional scaffold. The printing is performed with the computer-aided tissue engineering system according to a particular pattern. The micro-organ device comprises at least one micro-chamber each housing a micro-organ; and at least one microfluidic channel connected to the micro-chamber, wherein the micro-organ comprises cells arranged in a configuration that includes microscale spacing between portions of the cells to facilitate diffusion exchange between the cells and a medium supplied from the at least one microfluidic channel.

Micro-Ramp Flow Control for Oblique Shock Interactions: Comparisons of Computational and Experimental Data

NASA Technical Reports Server (NTRS)

Hirt, Stefanie M.; Reich, David B.; O'Connor, Michael B.

2010-01-01

Computational fluid dynamics was used to study the effectiveness of micro-ramp vortex generators to control oblique shock boundary layer interactions. Simulations were based on experiments previously conducted in the 15 x 15 cm supersonic wind tunnel at NASA Glenn Research Center. Four micro-ramp geometries were tested at Mach 2.0 varying the height, chord length, and spanwise spacing between micro-ramps. The overall flow field was examined. Additionally, key parameters such as boundary-layer displacement thickness, momentum thickness and incompressible shape factor were also examined. The computational results predicted the effects of the micro-ramps well, including the trends for the impact that the devices had on the shock boundary layer interaction. However, computing the shock boundary layer interaction itself proved to be problematic since the calculations predicted more pronounced adverse effects on the boundary layer due to the shock than were seen in the experiment.

A research factory for polymer microdevices: muFac

NASA Astrophysics Data System (ADS)

Anthony, Brian W.; Hardt, David E.; Hale, Melinda; Zarrouati, Nadege

2010-02-01

As part of our research on the manufacturing science of micron scale polymer-based devices, an automated production cell has been developed to explore its use in a volume manufacturing environment. This "micro-factory" allows the testing of models and hardware that have resulted from research on material characterization and simulation, tooling and equipment design and control, and process control and metrology. More importantly it has allowed us to identify the problems that exist between and within unit-processes. This paper details our efforts to produce basic micro-fluidic products in high volume at acceptable production rates and quality levels. The device chosen for our first product is a simple binary micromixer with 40×50 micron channel cross section manufactured by embossing of PMMA. The processes in the cell include laser cutting and drilling, hot embossing, thermal bonding and high-speed inspection of the components. Our goal is to create a "lights-out" factory that can make long production runs (e.g. an 8 hour shift) at high rates (Takt time of less than 3 minutes) with consistent quality. This contrasts with device foundries where prototypes in limited quantities but with high variety are the goal. Accordingly, rate and yield are dominant factors in this work, along with the need for precise material handling strategies. Production data will be presented to include process run charts, sampled functional testing of the products and measures of the overall system throughput.

Reasons of different colors in the ignimbrite lithology: micro-XRF and confocal Raman spectrometry method.

PubMed

Koralay, Tamer; Kadioglu, Yusuf Kagan

2008-03-01

Medium to large volume ignimbrites usually show vertical changes in terms of color, mineral components, texture and geochemistry. Determination of vertical changes in single extensive ignimbrite flow unit is difficult and requires careful studies. Color changes in ignimbrite flow units are very important for earth scientists. This may cause to identify the same ignimbrite series with different definition. Incesu ignimbrite has a wide distribution in the Central Anatolian Volcanic Province (CAVP). It is classified into three levels as lower, middle and upper according to color and welding degree. There is a sharp contact between the lower and middle level. The lower level is dark brown to black in color and the middle level has pinkish red to red color. The present paper focuses on the investigation of color changes between the ignimbrite levels by using micro-XRF and confocal Raman spectrometry. Micro-XRF and Raman spectrometry studies were performed on the polished thin sections of the lower and middle levels with different compositions. These differences were because of the compositional changes of K and slightly Fe elements distribution within the matrix. The dark brown to black color of the lower level was related to the high concentration of the K and Fe relatively to the middle level. Confocal Raman spectrometry investigations exhibited the matrix of the lower level mainly composed of anorthoclase, supporting the results of the micro-XRF.

Pore-Scale X-ray Micro-CT Imaging and Analysis of Oil Shales

NASA Astrophysics Data System (ADS)

Saif, T.

2015-12-01

The pore structure and the connectivity of the pore space during the pyrolysis of oil shales are important characteristics which determine hydrocarbon flow behaviour and ultimate recovery. We study the effect of temperature on the evolution of pore space and subsequent permeability on five oil shale samples: (1) Vernal Utah United States, (2) El Lajjun Al Karak Jordan, (3) Gladstone Queensland Australia (4) Fushun China and (5) Kimmerdige United Kingdom. Oil Shale cores of 5mm in diameter were pyrolized at 300, 400 and 500 °C. 3D imaging of 5mm diameter core samples was performed at 1μm voxel resolution using X-ray micro computed tomography (CT) and the evolution of the pore structures were characterized. The experimental results indicate that the thermal decomposition of kerogen at high temperatures is a major factor causing micro-scale changes in the internal structure of oil shales. At the early stage of pyrolysis, micron-scale heterogeneous pores were formed and with a further increase in temperature, the pores expanded and became interconnected by fractures. Permeability for each oil shale sample at each temperature was computed by simulation directly on the image voxels and by pore network extraction and simulation. Future work will investigate different samples and pursue insitu micro-CT imaging of oil shale pyrolysis to characterize the time evolution of the pore space.

Using a Virtual Manipulative Environment to Support Students' Organizational Structuring of Volume Units

ERIC Educational Resources Information Center

O'Dell, Jenna R.; Barrett, Jeffrey E.; Cullen, Craig J.; Rupnow, Theodore J.; Clements, Douglas H.; Sarama, Julie; Rutherford, George; Beck, Pamela S.

2017-01-01

In this study, we investigated how Grade 3 and 4 students' organizational structure for volume units develops through repeated experiences with a virtual manipulative for building prisms. Our data consist of taped clinical interviews within a micro-genetic experiment. We report on student strategy development using a virtual manipulative for…

Income Inequality across Micro and Meso Geographic Scales in the Midwestern United States, 1979-2009

ERIC Educational Resources Information Center

Peters, David J.

2012-01-01

This article examines the spatial distribution of income inequality and the socioeconomic factors affecting it using spatial analysis techniques across 16,285 block groups, 5,050 tracts, and 618 counties in the western part of the North Central Region of the United States. Different geographic aggregations result in different inequality outcomes,…

The effect of desferrioxamine on transferrin receptors, the cell cycle and growth rates of human leukaemic cells.

PubMed Central

Bomford, A; Isaac, J; Roberts, S; Edwards, A; Young, S; Williams, R

1986-01-01

The effect of the iron chelator, desferrioxamine, on transferrin binding, growth rates and the cell cycle was investigated in the human leukaemic cell line, K562. At all concentrations of the chelator (2-50 microM) binding of 125I-transferrin was increased by 24 h and reached a maximum at 72-96 h. Maximum binding (6-8-fold increased) occurred in cells treated with 20 microM-desferrioxamine, in contrast with control cells which, at 96 h, showed a 50% decrease over initial binding. Scatchard analysis at 4 degrees C showed that this increased binding was due to an increase in the number of receptors, as the Kd was similar in induced (1.8 nM) and control (1.5 nM) cells. After 96 h cells, cultured with 20 and 50 microM-desferrioxamine accumulated 59Fe from bovine transferrin at over twice the rate found with control cells, reflecting the increase in transferrin receptors. Although iron uptake was unimpaired by the chelator there was a dose-dependent inhibition of cell growth, with control cells completing three divisions in 96 h and those in 10 microM-desferrioxamine only two divisions. At the highest concentration (50 microM), cell division was abrogated although cell viability was maintained (85%). In contrast, DNA synthesis was not markedly affected, except at 50 microM-desferrioxamine when incorporation of [3H]thymidine was 52% of that in control cells. Flow cytometry revealed that there was a progressive accumulation of the cells in the active phases of their cycle (S, G2 + M). Desferrioxamine may increase transferrin receptors in two ways: by chelating a regulatory pool of iron within the cell, and by arresting cells in S phase when receptors are maximally expressed. PMID:3790074

Flexible Wing Base Micro Aerial Vehicles: Micro Air Vehicles (MAVs) for Surveillance and Remote Sensor Delivery

NASA Technical Reports Server (NTRS)

Ifju, Peter

2002-01-01

Micro Air Vehicles (MAVs) will be developed for tracking individuals, locating terrorist threats, and delivering remote sensors, for surveillance and chemical/biological agent detection. The tasks are: (1) Develop robust MAV platform capable of carrying sensor payload. (2) Develop fully autonomous capabilities for delivery of sensors to remote and distant locations. The current capabilities and accomplishments are: (1) Operational electric (inaudible) 6-inch MAVs with novel flexible wing, providing superior aerodynamic efficiency and control. (2) Vision-based flight stability and control (from on-board cameras).

Design and optimization of membrane-type acoustic metamaterials

NASA Astrophysics Data System (ADS)

Blevins, Matthew Grant

One of the most common problems in noise control is the attenuation of low frequency noise. Typical solutions require barriers with high density and/or thickness. Membrane-type acoustic metamaterials are a novel type of engineered material capable of high low-frequency transmission loss despite their small thickness and light weight. These materials are ideally suited to applications with strict size and weight limitations such as aircraft, automobiles, and buildings. The transmission loss profile can be manipulated by changing the micro-level substructure, stacking multiple unit cells, or by creating multi-celled arrays. To date, analysis has focused primarily on experimental studies in plane-wave tubes and numerical modeling using finite element methods. These methods are inefficient when used for applications that require iterative changes to the structure of the material. To facilitate design and optimization of membrane-type acoustic metamaterials, computationally efficient dynamic models based on the impedance-mobility approach are proposed. Models of a single unit cell in a waveguide and in a baffle, a double layer of unit cells in a waveguide, and an array of unit cells in a baffle are studied. The accuracy of the models and the validity of assumptions used are verified using a finite element method. The remarkable computational efficiency of the impedance-mobility models compared to finite element methods enables implementation in design tools based on a graphical user interface and in optimization schemes. Genetic algorithms are used to optimize the unit cell design for a variety of noise reduction goals, including maximizing transmission loss for broadband, narrow-band, and tonal noise sources. The tools for design and optimization created in this work will enable rapid implementation of membrane-type acoustic metamaterials to solve real-world noise control problems.

Research on equipment of micro-pressure measure and control in loading experiment of plant cell mechanics

NASA Astrophysics Data System (ADS)

Zhang, Lian; Yu, Chengbo; Tao, Hongyan; Chen, Xuejun; Zhai, Feng

2005-12-01

The equipment is developed to measure and control micro-pressure in loading experiment of plant cell mechanics. The motivation for the development of this equipment was to maintain a stationary micro-pressure on the agar of culturing cells to keep cytoactive in biology experiments. A singlechip controls the stepping motor of this equipment to drive loading equipment in the system, in order to load between 50mN and 250mN under a constant voltage. The accuracy is estimated to be +/-0.4 mN. The structure and control system of this equipment is introduced and described in detail. The experimental results show that the equipment is capable of maintaining a constant, stationary micropressure in cell culturing application and is worth of extending and applying.

Photothermally controlled Marangoni flow around a micro bubble

NASA Astrophysics Data System (ADS)

Namura, Kyoko; Nakajima, Kaoru; Kimura, Kenji; Suzuki, Motofumi

2015-01-01

We have experimentally investigated the control of Marangoni flow around a micro bubble using photothermal conversion. Using a focused laser spot acting as a highly localized heat source on Au nanoparticles/dielectric/Ag mirror thin film enables us to create a micro bubble and to control the temperature gradient around the bubble at a micrometer scale. When we irradiate the laser next to the bubble, a strong main flow towards the bubble and two symmetric rotation flows on either side of it develop. The shape of this rotation flow shows a significant transformation depending on the relative position of the bubble and the laser spot. Using this controllable rotation flow, we have demonstrated sorting of the polystyrene spheres with diameters of 2 μm and 0.75 μm according to their size.

A clinical study of ischaemic strokes with micro-albuminuria for risk stratification, short-term predictive value and outcome.

PubMed

Das, Sukdeb; Yadav, Ujjal; Ghosh, Kartik Chandra; Panchadhyayee, Sujoy; Kundu, Shib Shankar; Ganguly, Prasanta Kumar

2012-12-01

Stroke results more than 4.3 million deaths worldwide per annum and 85% of all strokes are ischaemic in nature. Besides numerous modifiable and non-modifiable known risk factors, microalbuminuria is thought to be an important marker of global endothelial dysfunction and associated with cardiovascular disease including stroke. Fifty ischaemic stroke cases and 50 (age, sex matched) control subjects were subjected to study to compare and evaluate risk stratification of micro-albuminuria, its predictive value and outcome on day 1 and day 7 among admitted ischaemic stroke cases.The result was found that micro-albuminuria was present in 66% of ischaemic stroke cases compared to only 8% of control group (p < 0.001). Most validated National Institute of Health Stroke Scale (NIHSS) score was used for evaluation and calculation of predictive value and outcome of micro-albuminuria positive patient where higher value indicates poor prognosis, and the result was mean NIHSS score 29.12 versus 18.88 between two groups of strokes ie, with and without micro-albuminuria. Out of 50 ischaemic stroke patients 33 (66%) had micro-albuminuria. Among 11 patients who died, 10 (90.9%) had micro-albuminuria and NIHSS score was 33.64 and 25.0 on day 1 and day 7. Among 39 patients who were discharged, 23 patients (58.97%) were MA positive and NIHSS score was much less than death group ie, 23.38 and 16.38 on day 1 and day 7 respectively. So this study reveals micro-albuminuria itself results higher risk for ischaemic stroke compared to control group and it shows good predictive value for early assessment of clinical severity and subsequent fatal outcome. This is also simple, cost effective and affordable.

Evaluation of lactic acid bacterium fermentation products and food-grade chemicals to control Listeria monocytogenes in blue crab (Callinectes sapidus) meat.

PubMed Central

Degnan, A J; Kaspar, C W; Otwell, W S; Tamplin, M L; Luchansky, J B

1994-01-01

Fresh blue crab (Callinectes sapidus) meat was obtained from retail markets in Florida and sampled for viable Listeria monocytogenes. The pathogen was found in crabmeat in three of four different lots tested by enrichment and at levels of 75 CFU/g in one of the same four lots by direct plating. Next, crabmeat was steam sterilized, inoculated with a three-strain mixture of L. monocytogenes (ca. 5.5 log10 CFU/g), washed with various lactic acid bacterium fermentation products (2,000 to 20,000 arbitrary units [AU]/ml of wash) or food-grade chemicals (0.25 to 4 M), and stored at 4 degrees C. Counts of the pathogen remained relatively constant in control samples during storage for 6 days, whereas in crabmeat washed with Perlac 1911 or MicroGard (10,000 to 20,000 AU), numbers initially decreased (0.5 to 1.0 log10 unit/g) but recovered to original levels within 6 days. Numbers of L. monocytogenes cells decreased 1.5 to 2.7 log10 units/g of crabmeat within 0.04 day when washed with 10,000 to 20,000 AU of Alta 2341, enterocin 1083, or Nisin per ml. Thereafter, counts increased 0.5 to 1.6 log10 units within 6 days. After washing with food-grade chemicals, modest reductions (0.4 to 0.8 log10 unit/g) were observed with sodium acetate (4 M), sodium diacetate (0.5 or 1 M), sodium lactate (1 M), or sodium nitrite (1.5 M). However, Listeria counts in crabmeat washed with 2 M sodium diacetate decreased 2.6 log10 units/g within 6 days. In addition, trisodium phosphate reduced L. monocytogenes counts from 1.7 (0.25 M) to > 4.6 (1 M) log10 units/g within 6 days.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:7944362

UltraSail - Ultra-Lightweight Solar Sail Concept

NASA Technical Reports Server (NTRS)

Burton, Rodney L.; Coverstone, Victoria L.; Hargens-Rysanek, Jennifer; Ertmer, Kevin M.; Botter, Thierry; Benavides, Gabriel; Woo, Byoungsam; Carroll, David L.; Gierow, Paul A.; Farmer, Greg

2005-01-01

UltraSail is a next-generation high-risk, high-payoff sail system for the launch, deployment, stabilization and control of very large (sq km class) solar sails enabling high payload mass fractions for high (Delta)V. Ultrasail is an innovative, non-traditional approach to propulsion technology achieved by combining propulsion and control systems developed for formation-flying micro-satellites with an innovative solar sail architecture to achieve controllable sail areas approaching 1 sq km, sail subsystem area densities approaching 1 g/sq m, and thrust levels many times those of ion thrusters used for comparable deep space missions. Ultrasail can achieve outer planetary rendezvous, a deep space capability now reserved for high-mass nuclear and chemical systems. One of the primary innovations is the near-elimination of sail supporting structures by attaching each blade tip to a formation-flying micro-satellite which deploys the sail, and then articulates the sail to provide attitude control, including spin stabilization and precession of the spin axis. These tip micro-satellites are controlled by 3-axis micro-thruster propulsion and an on-board metrology system. It is shown that an optimum spin rate exists which maximizes payload mass.

PECASE: Soaring Mechanisms for Flapping-Wing Micro Air Vehicles

DTIC Science & Technology

2015-03-31

2015 2. REPORT TYPE Final 4. TITLE AND SUBTITLE PECASE: Soaring mechanisms for flapping - wing micro air vehicles 6. AUTHOR(S) Robert J. Wood 3...N00014-10-1-0684 Award Title: "PECASE: Soaring mechanisms for flapping - wing micro air vehicles" [previous award: N00014-08-1-0919, "Hovering Control for...Insect-Inspired Flapping - Wing Micro Air Vehicles"] Final report a. Scientific and Technical Objectives The Harvard Microrobotics Lab has

Electrostatic Accelerometer for the Gravity Recovery and Climate Experiment Follow-On Mission (GRACE FO)

NASA Astrophysics Data System (ADS)

Lebat, V.; Foulon, B.; Christophe, B.

2013-12-01

The GRACE FO mission, led by the JPL (Jet Propulsion Laboratory), is an Earth-orbiting gravity mission, continuation of the GRACE mission, that will produce an accurate model of the Earth's gravity field variation providing global climatic data during five year at least. The mission involves two satellites in a loosely controlled tandem formation, with a micro-wave link measuring the inter-satellites distance variation. Non-uniformities in the distribution of the Earth's mass cause the distance between the two satellites to vary. This variation is measured to recover gravity, after subtracting the non-gravitational contributors, as the residual drag. ONERA (the French Aerospace Lab) is developing, manufacturing and testing electrostatic accelerometers measuring this residual drag applied on the satellites. The accelerometer is composed of two main parts: the Sensor Unit (including the Sensor Unit Mechanics and the Front-End Electronic Unit) and the Interface Control Unit. In the Accelerometer Core, located in the Sensor Unit Mechanics, the proof mass is levitated and maintained in a center of an electrode cage by electrostatic forces. Thus, any drag acceleration applied on the satellite involves a variation on the servo-controlled electrostatic suspension of the mass. The voltage on the electrodes providing this electrostatic force is the measurement output of the accelerometer. The impact of the accelerometer defaults (geometry, electronic and parasitic forces) leads to bias, misalignment and scale factor error, non-linearity and noise. Some of these accelerometer defaults are characterized by tests with micro-gravity pendulum bench and with drops in ZARM catapult. Besides, a thermal stability is needed for the accelerometer core and front-end electronics to avoid bias and scale factor variation, and reached by a thermal box designed by Astrium, spacecraft manufacturer. The accelerometers are designed to endure the launch vibrations and the thermal environment at ground and in orbit. As the measure must be accurate, no sliding of the core must appear in regard of the accelerometer external reference. To ensure the thermal core stability, the electrode cage of the core is made of glass material (ULE), which is very critical, in particular due to the free motion of the proof-mass during the launch. To assess the design of the accelerometer in particular the critical parts of the core, specific analysis is realized to ensure mechanical behavior. The design of electrostatic accelerometer of the GRACE Follow-On mission benefits of the GRACE heritage, GOCE launched in 2009 and MICROSCOPE which will be launched in 2016, including some improvement to improve the performance, in particular the thermal sensitivity of the measurements. The Preliminary Design Review of electronics was achieved successfully on July 2013, and the PDR of the whole instrument is forecasted on November 2013. The integration of the Engineering Model will begin on October 2013 and its status will be presented.

Migration of carbon dioxide included micro-nano bubble water in porous media and its monitoring

NASA Astrophysics Data System (ADS)

Takemura, T.; Hamamoto, S.; Suzuki, K.; Koichi, O.

2017-12-01

The distributed CO2 storage is the small scale storage and its located near the emission areas. In the distributed CO2 storage, the CO2 is neutralized by sediment and underground water in the subsurface region (300-500m depth). Carbon dioxide (CO2) included micro-nano bubbles is one approach in neutralizing CO2 and sediments by increasing CO2 volume per unit volume of water and accelerating the chemical reaction. In order to design underground treatment for CO2 gas in the subsurface, it is required to elucidate the behavior of CO2 included micro-nano bubbles in the water. In this study, we carried out laboratory experiment using the soil tank, and measure the amount of leakage of CO2 gas at the surface. In addition, the process of migration of carbon dioxide included micro-nano bubble was monitored by the nondestructive method, wave velocity and resistivity.

The MicroArray Quality Control (MAQC)-II study of common practices for the development and validation of microarray-based predictive models

EPA Science Inventory

The second phase of the MicroArray Quality Control (MAQC-II) project evaluated common practices for developing and validating microarray-based models aimed at predicting toxicological and clinical endpoints. Thirty-six teams developed classifiers for 13 endpoints - some easy, som...

Simulation and Flight Control of an Aeroelastic Fixed Wing Micro Aerial Vehicle

NASA Technical Reports Server (NTRS)

Waszak, Martin; Davidson, John B.; Ifju, Peter G.

2002-01-01

Micro aerial vehicles have been the subject of continued interest and development over the last several years. The majority of current vehicle concepts rely on rigid fixed wings or rotors. An alternate design based on an aeroelastic membrane wing has also been developed that exhibits desired characteristics in flight test demonstrations, competition, and in prior aerodynamics studies. This paper presents a simulation model and an assessment of flight control characteristics of the vehicle. Linear state space models of the vehicle associated with typical trimmed level flight conditions and which are suitable for control system design are presented as well. The simulation is used as the basis for the design of a measurement based nonlinear dynamic inversion control system and outer loop guidance system. The vehicle/controller system is the subject of ongoing investigations of autonomous and collaborative control schemes. The results indicate that the design represents a good basis for further development of the micro aerial vehicle for autonomous and collaborative controls research.

Real-Time Variable Rate Spraying in Orchards and Vineyards: A Review

NASA Astrophysics Data System (ADS)

Wandkar, Sachin Vilas; Bhatt, Yogesh Chandra; Jain, H. K.; Nalawade, Sachin M.; Pawar, Shashikant G.

2018-06-01

Effective and efficient use of pesticides in the orchards is of concern since many years. With the conventional constant rate sprayers, equal dose of pesticide is applied to each tree. Since, there is great variation in size and shape of each tree in the orchard, trees gets either oversprayed or undersprayed. Real-time variable rate spraying technology offers pesticide application in accordance with tree size. With the help of suitable sensors, tree characteristics such as canopy volume, foliage density, etc. can be acquired and with the micro-processing unit coupled with proper algorithm, flow of electronic proportional valves can be controlled thus, controlling the flow rate of nozzles according to tree characteristics. Also, sensors can help in the detection of spaces in-between trees which allows to control the spray in spaces. Variable rate spraying helps in achieving precision in spraying operation especially inside orchards. This paper reviews the real-time variable rate spraying technology and efforts made by the various researchers for real-time variable application in the orchards and vineyards.

Modeling inter-signal arrival times for accurate detection of CAN bus signal injection attacks

DOE Office of Scientific and Technical Information (OSTI.GOV)

Moore, Michael Roy; Bridges, Robert A; Combs, Frank L

Modern vehicles rely on hundreds of on-board electronic control units (ECUs) communicating over in-vehicle networks. As external interfaces to the car control networks (such as the on-board diagnostic (OBD) port, auxiliary media ports, etc.) become common, and vehicle-to-vehicle / vehicle-to-infrastructure technology is in the near future, the attack surface for vehicles grows, exposing control networks to potentially life-critical attacks. This paper addresses the need for securing the CAN bus by detecting anomalous traffic patterns via unusual refresh rates of certain commands. While previous works have identified signal frequency as an important feature for CAN bus intrusion detection, this paper providesmore » the first such algorithm with experiments on five attack scenarios. Our data-driven anomaly detection algorithm requires only five seconds of training time (on normal data) and achieves true positive / false discovery rates of 0.9998/0.00298, respectively (micro-averaged across the five experimental tests).« less

Real-Time Variable Rate Spraying in Orchards and Vineyards: A Review

NASA Astrophysics Data System (ADS)

Wandkar, Sachin Vilas; Bhatt, Yogesh Chandra; Jain, H. K.; Nalawade, Sachin M.; Pawar, Shashikant G.

2018-02-01

Effective and efficient use of pesticides in the orchards is of concern since many years. With the conventional constant rate sprayers, equal dose of pesticide is applied to each tree. Since, there is great variation in size and shape of each tree in the orchard, trees gets either oversprayed or undersprayed. Real-time variable rate spraying technology offers pesticide application in accordance with tree size. With the help of suitable sensors, tree characteristics such as canopy volume, foliage density, etc. can be acquired and with the micro-processing unit coupled with proper algorithm, flow of electronic proportional valves can be controlled thus, controlling the flow rate of nozzles according to tree characteristics. Also, sensors can help in the detection of spaces in-between trees which allows to control the spray in spaces. Variable rate spraying helps in achieving precision in spraying operation especially inside orchards. This paper reviews the real-time variable rate spraying technology and efforts made by the various researchers for real-time variable application in the orchards and vineyards.

Impact of the irregular microgeometry of polyurethane foam on the macroscopic acoustic behavior predicted by a unit-cell model.

PubMed

Doutres, O; Ouisse, M; Atalla, N; Ichchou, M

2014-10-01

This paper deals with the prediction of the macroscopic sound absorption behavior of highly porous polyurethane foams using two unit-cell microstructure-based models recently developed by Doutres, Atalla, and Dong [J. Appl. Phys. 110, 064901 (2011); J. Appl. Phys. 113, 054901 (2013)]. In these models, the porous material is idealized as a packing of a tetrakaidecahedra unit-cell representative of the disordered network that constitutes the porous frame. The non-acoustic parameters involved in the classical Johnson-Champoux-Allard model (i.e., porosity, airflow resistivity, tortuosity, etc.) are derived from characteristic properties of the unit-cell and semi-empirical relationships. A global sensitivity analysis is performed on these two models in order to investigate how the variability associated with the measured unit-cell characteristics affects the models outputs. This allows identification of the possible limitations of a unit-cell micro-macro approach due to microstructure irregularity. The sensitivity analysis mainly shows that for moderately and highly reticulated polyurethane foams, the strut length parameter is the key parameter since it greatly impacts three important non-acoustic parameters and causes large uncertainty on the sound absorption coefficient even if its measurement variability is moderate. For foams with a slight inhomogeneity and anisotropy, a micro-macro model associated to cell size measurements should be preferred.

CROSS-DISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY: Surface Micromachined Adjustable Micro-Concave Mirror for Bio-Detection Applications

NASA Astrophysics Data System (ADS)

Kuo, Ju-Nan; Chen, Wei-Lun; Jywe, Wen-Yuh

2009-08-01

We present a bio-detection system integrated with an adjustable micro-concave mirror. The bio-detection system consists of an adjustable micro-concave mirror, micro flow cytometer chip and optical detection module. The adjustable micro-concave mirror can be fabricated with ease using commercially available MEMS foundry services (such as multiuser MEMS processes, MUMPs) and its curvature can be controlled utilizing thermal or electrical effects. Experimental results show that focal lengths of the micro-concave mirror ranging from 313.5 to 2275.0 μm are achieved. The adjustable micro-concave mirror can be used to increase the efficiency of optical detection and provide a high signal-to-noise ratio. The developed micro-concave mirror is integrated with a micro flow cytometer for cell counting applications. Successful counting of fluorescent-labeled beads is demonstrated using the developed method.

The Plant Research Unit: An International Space Station Habitat

NASA Technical Reports Server (NTRS)

Morrow, Robert; Reiss-Bubenheim, Debra; Schaefer, Ronald L.

2003-01-01

The Plant Research Unit (PRU) is one of six life science habitats being developed as part of the Space Station Biological Research Program. The PRU is designed for experiments in microgravity and will utilize the ISS Centrifuge Facility to provide gravity levels between microgravity and 29. The PRU will provide and control all aspects of a plant s needs in a nearly closed system. In other words, the shoot and root environments will not be open to the astronaut s environment except for experiment maintenance such as planting, harvesting and plant sampling. This also means that all lighting, temperature and humidity control, "watering," and air filtering and cleaning .must be done within strict limitations of volume, weight, power, and crew time while at the same time providing a very high level of reliability and a service life in excess of 10 years. The PRU will contain two plant chambers 31.5 cm tall, each with independent control of temperature, humidity, light level and photoperiod, CO2 level, nutrient and water delivery, and video and data acquisition. The PRU is currently in the preliminary design phase and a number of subsystem components have been prototyped for testing, including the temperature and humidity control systems, the plant chambers, the LED lighting system, the atmospheric control system and a variety of nutrient delivery systems. The LED prototype provides independent feedback control of 5 separate spectral bands and variable output between 0 and 1000 micro-mol sq m/sec. The water and nutrient delivery system (WNDS) prototypes have been used to test particulate based, thin film, and gel-based WNDS configurations.

9 CFR 114.5 - Micro-organisms used as seed.

Code of Federal Regulations, 2013 CFR

2013-01-01

... BIOLOGICAL PRODUCTS § 114.5 Micro-organisms used as seed. Micro-organisms used in the preparation of biological products at licensed establishments shall be free from the causative agents of other diseases or... Management and Budget under control number 0579-0059) [39 FR 16869, May 10, 1974, as amended at 48 FR 57473...

9 CFR 114.5 - Micro-organisms used as seed.

Code of Federal Regulations, 2012 CFR

2012-01-01

... BIOLOGICAL PRODUCTS § 114.5 Micro-organisms used as seed. Micro-organisms used in the preparation of biological products at licensed establishments shall be free from the causative agents of other diseases or... Management and Budget under control number 0579-0059) [39 FR 16869, May 10, 1974, as amended at 48 FR 57473...

9 CFR 114.5 - Micro-organisms used as seed.

Code of Federal Regulations, 2014 CFR

2014-01-01

... BIOLOGICAL PRODUCTS § 114.5 Micro-organisms used as seed. Micro-organisms used in the preparation of biological products at licensed establishments shall be free from the causative agents of other diseases or... Management and Budget under control number 0579-0059) [39 FR 16869, May 10, 1974, as amended at 48 FR 57473...

9 CFR 114.5 - Micro-organisms used as seed.

Code of Federal Regulations, 2010 CFR

2010-01-01

... BIOLOGICAL PRODUCTS § 114.5 Micro-organisms used as seed. Micro-organisms used in the preparation of biological products at licensed establishments shall be free from the causative agents of other diseases or... Management and Budget under control number 0579-0059) [39 FR 16869, May 10, 1974, as amended at 48 FR 57473...

9 CFR 114.5 - Micro-organisms used as seed.

Code of Federal Regulations, 2011 CFR

2011-01-01

... BIOLOGICAL PRODUCTS § 114.5 Micro-organisms used as seed. Micro-organisms used in the preparation of biological products at licensed establishments shall be free from the causative agents of other diseases or... Management and Budget under control number 0579-0059) [39 FR 16869, May 10, 1974, as amended at 48 FR 57473...

The potential of hybrid micro-vortex generators to control flow separation of NACA 4415 airfoil in subsonic flow

NASA Astrophysics Data System (ADS)

Jumahadi, Muhammad Taufiq; Saad, Mohd Rashdan; Idris, Azam Che; Sujipto, Suriyadi; Rahman, Mohd Rosdzimin Abdul

2018-02-01

Boundary layer separation is detrimental to the lift and drag of most aeronautical applications. Many vortex generators (VG), both passive and active have been designed to reduce these drawbacks. This study targets to investigate the effectiveness of hybrid micro-VGs, which combine both active and passive micro-VGs in controlling separation under subsonic conditions. NACA 4415 airfoils installed with passive, active and hybrid micro-VGs each are designed, 3D printed, and tested in a wind tunnel at 26.19 m/s under Re = 2.5x105. The lift and drag measurements from a 3-component force balance prove that hybrid micro-VGs increase lift by up to 21.2%, increase drag by more than 11.3% and improve lift-to-drag ratio by at least 8.6% until up to 33.7%. From this research, it is believed that hybrid micro-VGs are competitive to the performance of active VGs and a better configuration is to be considered to reduce parasitic drag and outstand active VGs.

Angular dependant micro-ESR characterization of a locally doped Gd3+:Al2O3 hybrid system for quantum applications

NASA Astrophysics Data System (ADS)

Wisby, I. S.; de Graaf, S. E.; Gwilliam, R.; Adamyan, A.; Kubatkin, S. E.; Meeson, P. J.; Tzalenchuk, A. Ya.; Lindstrom, T.

Rare-earth doped crystals interfaced with superconducting quantum circuitry are an attractive platform for quantum memory and transducer applications. Here we present a detailed characterization of a locally implanted Gd3+ in Al2O3 system coupled to a superconducting micro-resonator, by performing angular dependent micro-electron-spin-resonance (micro-ESR) measurements at mK temperatures. The device is fabricated using a hard Si3N4 mask to facilitate a local ion-implantation technique for precision control of the dopant location. The technique is found not to degrade the internal quality factor of the resonators which remains above 105 (1). We find the measured angular dependence of the micro-ESR spectra to be in excellent agreement with the modelled Hamiltonian, supporting the conclusion that the dopant ions are successfully integrated into their relevant lattice sites whilst maintaining crystalline symmetries. Furthermore, we observe clear contributions from individual microwave field components of our micro-resonator, emphasising the need for controllable local implantation. 1 Wisby et al. Appl. Phys. Lett. 105, 102601 (2014)

Fabrication of core-shell micro/nanoparticles for programmable dual drug release by emulsion electrospraying

NASA Astrophysics Data System (ADS)

Wang, Yazhou; Zhang, Yiqiong; Wang, Bochu; Cao, Yang; Yu, Qingsong; Yin, Tieying

2013-06-01

The study aimed at constructing a novel drug delivery system for programmable multiple drug release controlled with core-shell structure. The core-shell structure consisted of chitosan nanoparticles as core and polyvinylpyrrolidone micro/nanocoating as shell to form core-shell micro/nanoparticles, which was fabricated by ionic gelation and emulsion electrospray methods. As model drug agents, Naproxen and rhodamine B were encapsulated in the core and shell regions, respectively. The core-shell micro/nanoparticles thus fabricated were characterized and confirmed by scanning electron microscope, transmission electron microscope, and fluorescence optical microscope. The core-shell micro/nanoparticles showed good release controllability through drug release experiment in vitro. It was noted that a programmable release pattern for dual drug agents was also achieved by adjusting their loading regions in the core-shell structures. The results indicate that emulsion electrospraying technology is a promising approach in fabrication of core-shell micro/nanoparticles for programmable dual drug release. Such a novel multi-drug delivery system has a potential application for the clinical treatment of cancer, tuberculosis, and tissue engineering.

Molecular Mechanism of MicroRNA-200c Regulating Transforming Growth Factor-β (TGF-β)/SMAD Family Member 3 (SMAD3) Pathway by Targeting Zinc Finger E-Box Binding Homeobox 1 (ZEB1) in Hypospadias in Rats.

PubMed

Qian, Chong; Dang, Xiangyang; Wang, Xianglin; Xu, Wei; Pang, Guijian; Chen, Yifeng; Liu, Chengbei

2016-10-29

BACKGROUND The aim of this study was to explore effects of microRNA-200c regulating TGF-β/Smad3 pathway by targeting Zeb1 on the occurrence and development of hypospadias and to evaluate the relationship between microRNA-200c and occurrence of hypospadias. MATERIAL AND METHODS Pregnant rats with a gestational age of 12 days were allocated into 2 groups; one received gavage of DEHP-contained soybean oil (1 ml/day, 8 days; Group A) and the other had gavage of normal soybean oil (1 ml/day, 8 days; Group B). Baby rats with hypospadias from Group A were assigned to the model group (n=20) and healthy baby rats from Group B were assigned to the control group (n=20). Real-time quantitative polymerase chain reaction (qRT-PCR), immunohistochemistry and Western blot analysis were performed to detect microRNA-200c, Zeb1, TGF-β, and Smad3 mRNA and protein expressions in the model group (n=20) and the control group (n=20). The relationship between microRNA-200c and Zeb1 was detected using a dual-luciferase reporter gene experiment. After the in vitro intervention experiment in fetal rat penises, Western blot was used to detect the expression of Zeb1, TGF-β, and Smad3. RESULTS In the model group, microRNA-200c was expressed at a low level, and microRNA-200c expression in control group was 2.1 times higher than in the model group (P<0.05). When compared with the control group, mRNA expressions, protein expressions, and positive rates of Zeb1, TGF-β, and Smad3 were higher in the model group (all P<0.01). Luciferase gene report determined that Zeb1 is a target gene of microRNA-200c. The in vitro intervention experiment in fetal rat penises found that a high concentration of microRNA-200c inhibited hypospadias occurrence by suppressing the expression of Zeb1, TGF-β, and Smad3. CONCLUSIONS MicroRNA-200c was expressed in hypospadias penis tissues at low levels and was negatively correlated with Zeb1 expression. MicroRNA-200c up-regulated Zeb1 expression to regulate the TGF-β/Smad3 pathway, which led to the occurrence of hypospadias.

Molecular Mechanism of MicroRNA-200c Regulating Transforming Growth Factor-β (TGF-β)/SMAD Family Member 3 (SMAD3) Pathway by Targeting Zinc Finger E-Box Binding Homeobox 1 (ZEB1) in Hypospadias in Rats

PubMed Central

Qian, Chong; Dang, Xiangyang; Wang, Xianglin; Xu, Wei; Pang, Guijian; Chen, Yifeng; Liu, Chengbei

2016-01-01

Background The aim of this study was to explore effects of microRNA-200c regulating TGF-β/Smad3 pathway by targeting Zeb1 on the occurrence and development of hypospadias and to evaluate the relationship between microRNA-200c and occurrence of hypospadias. Material/Methods Pregnant rats with a gestational age of 12 days were allocated into 2 groups; one received gavage of DEHP-contained soybean oil (1 ml/day, 8 days; Group A) and the other had gavage of normal soybean oil (1 ml/day, 8 days; Group B). Baby rats with hypospadias from Group A were assigned to the model group (n=20) and healthy baby rats from Group B were assigned to the control group (n=20). Real-time quantitative polymerase chain reaction (qRT-PCR), immunohistochemistry and Western blot analysis were performed to detect microRNA-200c, Zeb1, TGF-β, and Smad3 mRNA and protein expressions in the model group (n=20) and the control group (n=20). The relationship between microRNA-200c and Zeb1 was detected using a dual-luciferase reporter gene experiment. After the in vitro intervention experiment in fetal rat penises, Western blot was used to detect the expression of Zeb1, TGF-β, and Smad3. Results In the model group, microRNA-200c was expressed at a low level, and microRNA-200c expression in control group was 2.1 times higher than in the model group (P<0.05). When compared with the control group, mRNA expressions, protein expressions, and positive rates of Zeb1, TGF-β, and Smad3 were higher in the model group (all P<0.01). Luciferase gene report determined that Zeb1 is a target gene of microRNA-200c. The in vitro intervention experiment in fetal rat penises found that a high concentration of microRNA-200c inhibited hypospadias occurrence by suppressing the expression of Zeb1, TGF-β, and Smad3. Conclusions MicroRNA-200c was expressed in hypospadias penis tissues at low levels and was negatively correlated with Zeb1 expression. MicroRNA-200c up-regulated Zeb1 expression to regulate the TGF-β/Smad3 pathway, which led to the occurrence of hypospadias. PMID:27794206

High-Performance Substrates for SERS Detection via Microphotonic Photopolymer Characterization and Coating With Functionalized Hydrogels

DTIC Science & Technology

2006-11-26

with controlled micro and nanostructure for highly selective, high sensitivity assays. The process was modeled and a procedure for fabricating SERS...small volumes with controlled micro and nanostructure for highly selective, high sensitivity assays. We proved the feasibility of the technique and...films templated by colloidal crystals. The control over the film structure allowed optimizing their performance for potential sensor applications. The

Automation of the micro-arc oxidation process

NASA Astrophysics Data System (ADS)

Golubkov, P. E.; Pecherskaya, E. A.; Karpanin, O. V.; Shepeleva, Y. V.; Zinchenko, T. O.; Artamonov, D. V.

2017-11-01

At present the significantly increased interest in micro-arc oxidation (MAO) encourages scientists to look for the solution of the problem of this technological process controllability. To solve this problem an automated technological installation MAO was developed, its structure and control principles are presented in this article. This device will allow to provide the controlled synthesis of MAO coatings and to identify MAO process patterns which contributes to commercialization of this technology.

An Empirical Study on Operator Interface Design for Handheld Devices to Control Micro Aerial Vehicles

DTIC Science & Technology

2010-10-01

An Empirical Study on Operator Interface Design for Handheld Devices to Control Micro Aerial Vehicles Ming Hou...Report DRDC Toronto TR 2010-075 October 2010 An Empirical Study on Operator Interface Design for Handheld Devices to...drives the need for a small and light controller which will not hinder a soldier carrying it. This requirement brings an issue of designing an

Blood-pool contrast agent for pre-clinical computed tomography

NASA Astrophysics Data System (ADS)

Cruje, Charmainne; Tse, Justin J.; Holdsworth, David W.; Gillies, Elizabeth R.; Drangova, Maria

2017-03-01

Advances in nanotechnology have led to the development of blood-pool contrast agents for micro-computed tomography (micro-CT). Although long-circulating nanoparticle-based agents exist for micro-CT, they are predominantly based on iodine, which has a low atomic number. Micro-CT contrast increases when using elements with higher atomic numbers (i.e. lanthanides), particularly at higher energies. The purpose of our work was to develop and evaluate a lanthanide-based blood-pool contrast agent that is suitable for in vivo micro-CT. We synthesized a contrast agent in the form of polymer-encapsulated Gd nanoparticles and evaluated its stability in vitro. The synthesized nanoparticles were shown to have an average diameter of 127 +/- 6 nm, with good size dispersity. Particle size distribution - evaluated by dynamic light scattering over the period of two days - demonstrated no change in size of the contrast agent in water and saline. Additionally, our contrast agent was stable in a mouse serum mimic for up to 30 minutes. CT images of the synthesized contrast agent (containing 27 mg/mL of Gd) demonstrated an attenuation of over 1000 Hounsfield Units. This approach to synthesizing a Gd-based blood-pool contrast agent promises to enhance the capabilities of micro-CT imaging.

Self-assembled polymeric nanocarriers for the targeted delivery of retinoic acid to the hair follicle

NASA Astrophysics Data System (ADS)

Lapteva, Maria; Möller, Michael; Gurny, Robert; Kalia, Yogeshvar N.

2015-11-01

Acne vulgaris is a highly prevalent dermatological disease of the pilosebaceous unit (PSU). An inability to target drug delivery to the PSU results in poor treatment efficacy and the incidence of local side-effects. Cutaneous application of nanoparticulate systems is reported to induce preferential accumulation in appendageal structures. The aim of this work was to prepare stable polymeric micelles containing retinoic acid (RA) using a biodegradable and biocompatible diblock methoxy-poly(ethylene glycol)-poly(hexylsubstituted lactic acid) copolymer (MPEG-dihexPLA) and to evaluate their ability to deliver RA to skin. An innovative punch biopsy sample preparation method was developed to selectively quantify follicular delivery; the amounts of RA present were compared to those in bulk skin, (i.e. without PSU), which served as the control. RA was successfully incorporated into micelle nanocarriers and protected from photoisomerization by inclusion of Quinoline Yellow. Incorporation into the spherical, homogeneous and nanometer-scale micelles (dn < 20 nm) increased the aqueous solubility of RA by >400-fold. Drug delivery experiments in vitro showed that micelles were able to deliver RA to porcine and human skins more efficiently than Retin-A® Micro (0.04%), a marketed gel containing RA loaded microspheres, (7.1 +/- 1.1% vs. 0.4 +/- 0.1% and 7.5 +/- 0.8% vs. 0.8 +/- 0.1% of the applied dose, respectively). In contrast to a non-colloidal RA solution, Effederm® (0.05%), both the RA loaded MPEG-dihexPLA polymeric micelles (0.005%) and Retin-A® Micro (0.04%) displayed selectivity for delivery to the PSU with 2-fold higher delivery to PSU containing samples than to control samples. Moreover, the micelle formulation outperformed Retin-A® Micro in terms of delivery efficiency to PSU presenting human skin (10.4 +/- 3.2% vs. 0.6 +/- 0.2%, respectively). The results indicate that the polymeric micelle formulation enabled an increased and targeted delivery of RA to the PSU, potentially translating to a safer and more efficient clinical management of acne.Acne vulgaris is a highly prevalent dermatological disease of the pilosebaceous unit (PSU). An inability to target drug delivery to the PSU results in poor treatment efficacy and the incidence of local side-effects. Cutaneous application of nanoparticulate systems is reported to induce preferential accumulation in appendageal structures. The aim of this work was to prepare stable polymeric micelles containing retinoic acid (RA) using a biodegradable and biocompatible diblock methoxy-poly(ethylene glycol)-poly(hexylsubstituted lactic acid) copolymer (MPEG-dihexPLA) and to evaluate their ability to deliver RA to skin. An innovative punch biopsy sample preparation method was developed to selectively quantify follicular delivery; the amounts of RA present were compared to those in bulk skin, (i.e. without PSU), which served as the control. RA was successfully incorporated into micelle nanocarriers and protected from photoisomerization by inclusion of Quinoline Yellow. Incorporation into the spherical, homogeneous and nanometer-scale micelles (dn < 20 nm) increased the aqueous solubility of RA by >400-fold. Drug delivery experiments in vitro showed that micelles were able to deliver RA to porcine and human skins more efficiently than Retin-A® Micro (0.04%), a marketed gel containing RA loaded microspheres, (7.1 +/- 1.1% vs. 0.4 +/- 0.1% and 7.5 +/- 0.8% vs. 0.8 +/- 0.1% of the applied dose, respectively). In contrast to a non-colloidal RA solution, Effederm® (0.05%), both the RA loaded MPEG-dihexPLA polymeric micelles (0.005%) and Retin-A® Micro (0.04%) displayed selectivity for delivery to the PSU with 2-fold higher delivery to PSU containing samples than to control samples. Moreover, the micelle formulation outperformed Retin-A® Micro in terms of delivery efficiency to PSU presenting human skin (10.4 +/- 3.2% vs. 0.6 +/- 0.2%, respectively). The results indicate that the polymeric micelle formulation enabled an increased and targeted delivery of RA to the PSU, potentially translating to a safer and more efficient clinical management of acne. Electronic supplementary information (ESI) available. See DOI: 10.1039/c5nr04770f

MicroPPT-Based Secondary/Backup ACS for a 160-m, 450-kg Solar Sail Spacecraft

NASA Technical Reports Server (NTRS)

Wie, Bong; Murphy, David

2005-01-01

Solar sail tip-mounted, lightweight pulsed plasma thrusters (PPTs) are proposed for a secondary (or backup) attitude control system (ACS) of a 160-m, 450-kg solar sail spacecraft of the Solar Polar Imager (SPI) mission. A propellantless primary ACS of the SPI sailcraft employs trim control masses running along mast lanyards for pitch/yaw control together with roll stabilizer bars at the mast tips for quadrant tilt (roll) control. The robustness of such a propellantless primary ACS would be further enhanced by a secondary ACS utilizing tip-mounted, lightweight PPTs. The microPPT-based ACS is intended mainly for attitude recovery maneuvers from various off-nominal conditions that cannot be reliably handled by the propellantless primary ACS. However, it can also be employed for: i) the checkout or standby mode prior to and during sail deployment, ii) the post-deployment transition mode (prior to the propellantless primary ACS mode operation), iii) the solar sailing cruise mode of a trimmed sailcraft, and iv) the spin-stabilized, sun-pointing, safe mode. Although a conventional bus ACS is required for the SPI mission as the sail is jettisoned at the start of its science mission phase, the microPPT-based ACS option promises greater redundancy and robustness for the SPI mission. For other sailing missions, where the sail is never jettisoned, this secondary ACS provides a lower-cost, lower-mass propulsion for deployment control and greater redundancy than any traditional reaction-jet control system. This paper presents an overview nf the state--of-the--art microPPT technology, the design requirements of microPPTs for solar sail attitude control, and the preliminary ACS design and simulation results.

Millimeter scale robots for the nanofactory

NASA Astrophysics Data System (ADS)

Murthy, Rakesh

The top down approach is a commonly employed miniaturization pathway into micro and nanomanufacturing. Its popularity is due to the fact that it adapts traditionally engineered macro scale positioning, manipulation and processing technology with micro and nano scale precision and part sizes. However, state of the art top down systems such as the Atomic Force Microscope (AFM) span four to five orders of magnitude larger than the parts being handled. This dissertation addresses the need for creating millimeter size robotic positioning technology that closes the size gap between equipment and part sizes. Such microrobot manufacturing methodology comprising of micro component-level design, fabrication and high yield assembly, system-level packaging, modeling, precision evaluation and control is presented and exemplified using two classes of microrobots. Both microrobots incorporate Micro Electro Mechanical Systems (MEMS) to combine high precision and low foot-print. The first microrobot type, the "ARRIpede" is a multi legged autonomous crawler, and is designed to operate as a mobile unit enabling parts transfer in a nanoassembly environment. An embodiment of this microrobot is demonstrated for planar motions with three degrees of freedom (XYtheta). The microrobot consists of a MEMS die "belly" spanning 10mm x 10mm x 1mm with in-plane electrothermal actuators and vertically assembled legs, and an electronic "backpack" spanning 15mmx15mmx10mm to generate a leg gait sequence. By incorporating bulk micromachined parts and precise epoxy dispensing at the assembled leg joint, the microrobot has a high payload bearing capacity (at least 9g). Simulations with a nonholonomic robot predict microcrawler velocities of a few mm/s under realistic assumptions. The open loop crawling velocity is experimentally characterized for various actuator frequencies and a close match with simulations is observed. A Linear Quadratic Regulator (LQR) based controller consisting of a high magnification camera and a laser displacement sensor for feedback is implemented. The open/closed loop positioning repeatabilities are evaluated and compared. The second micro robot called the "AFAM" (Articulated Four Axes Micro Robot) is a fixed base articulated design targeting micro and nano scale manipulation and probing applications. An embodiment of this microrobot is constructed incorporating four degrees of freedom (X, Y, Pitch and Yaw), occupying a total volume of 3mm x 2mm x 1mm, and operating within a workspace envelope of 50mum x 50mum x 75mum. This is by far the largest operating envelope of any other independent MEMS positioner with non-planar dexterity. A cable based transmission and motion amplification mechanism is designed to achieve the pitch and yaw degrees of freedom. The de-coupled motion of the microrobot is achieved by kinematic identification of the Jacobian and using a 3D flexure based kinematic model of the microrobot. By using the derived kinematics, the microrobot is driven to create nanoindents on a polymer surface. The end-effector positioning accuracy, repeatability and resolution are characterized using the nanoindents.

miR-Sens--a retroviral dual-luciferase reporter to detect microRNA activity in primary cells.

PubMed

Beillard, Emmanuel; Ong, Siau Chi; Giannakakis, Antonis; Guccione, Ernesto; Vardy, Leah A; Voorhoeve, P Mathijs

2012-05-01

MicroRNA-mRNA interactions are commonly validated and deconstructed in cell lines transfected with luciferase reporters. However, due to cell type-specific variations in microRNA or RNA-binding protein abundance, such assays may not reliably reflect microRNA activity in other cell types that are less easily transfected. In order to measure miRNA activity in primary cells, we constructed miR-Sens, a MSCV-based retroviral vector that encodes both a Renilla luciferase reporter gene controlled by microRNA binding sites in its 3' UTR and a Firefly luciferase normalization gene. miR-Sens sensors can be efficiently transduced in primary cells such as human fibroblasts and mammary epithelial cells, and allow the detection of overexpressed and, more importantly, endogenous microRNAs. Notably, we find that the relative luciferase activity is correlated to the miRNA expression, allowing quantitative measurement of microRNA activity. We have subsequently validated the miR-Sens 3' UTR vectors with known human miRNA-372, miRNA-373, and miRNA-31 targets (LATS2 and TXNIP). Overall, we observe that miR-Sens-based assays are highly reproducible, allowing detection of the independent contribution of multiple microRNAs to 3' UTR-mediated translational control of LATS2. In conclusion, miR-Sens is a new tool for the efficient study of microRNA activity in primary cells or panels of cell lines. This vector will not only be useful for studies on microRNA biology, but also more broadly on other factors influencing the translation of mRNAs.

miR-Sens—a retroviral dual-luciferase reporter to detect microRNA activity in primary cells

PubMed Central

Beillard, Emmanuel; Ong, Siau Chi; Giannakakis, Antonis; Guccione, Ernesto; Vardy, Leah A.; Voorhoeve, P. Mathijs

2012-01-01

MicroRNA–mRNA interactions are commonly validated and deconstructed in cell lines transfected with luciferase reporters. However, due to cell type-specific variations in microRNA or RNA-binding protein abundance, such assays may not reliably reflect microRNA activity in other cell types that are less easily transfected. In order to measure miRNA activity in primary cells, we constructed miR-Sens, a MSCV-based retroviral vector that encodes both a Renilla luciferase reporter gene controlled by microRNA binding sites in its 3′ UTR and a Firefly luciferase normalization gene. miR-Sens sensors can be efficiently transduced in primary cells such as human fibroblasts and mammary epithelial cells, and allow the detection of overexpressed and, more importantly, endogenous microRNAs. Notably, we find that the relative luciferase activity is correlated to the miRNA expression, allowing quantitative measurement of microRNA activity. We have subsequently validated the miR-Sens 3′ UTR vectors with known human miRNA-372, miRNA-373, and miRNA-31 targets (LATS2 and TXNIP). Overall, we observe that miR-Sens-based assays are highly reproducible, allowing detection of the independent contribution of multiple microRNAs to 3′ UTR–mediated translational control of LATS2. In conclusion, miR-Sens is a new tool for the efficient study of microRNA activity in primary cells or panels of cell lines. This vector will not only be useful for studies on microRNA biology, but also more broadly on other factors influencing the translation of mRNAs. PMID:22417692

Keeping a Good Attitude: A Quaternion-Based Orientation Filter for IMUs and MARGs

PubMed Central

Valenti, Roberto G.; Dryanovski, Ivan; Xiao, Jizhong

2015-01-01

Orientation estimation using low cost sensors is an important task for Micro Aerial Vehicles (MAVs) in order to obtain a good feedback for the attitude controller. The challenges come from the low accuracy and noisy data of the MicroElectroMechanical System (MEMS) technology, which is the basis of modern, miniaturized inertial sensors. In this article, we describe a novel approach to obtain an estimation of the orientation in quaternion form from the observations of gravity and magnetic field. Our approach provides a quaternion estimation as the algebraic solution of a system from inertial/magnetic observations. We separate the problems of finding the “tilt” quaternion and the heading quaternion in two sub-parts of our system. This procedure is the key for avoiding the impact of the magnetic disturbances on the roll and pitch components of the orientation when the sensor is surrounded by unwanted magnetic flux. We demonstrate the validity of our method first analytically and then empirically using simulated data. We propose a novel complementary filter for MAVs that fuses together gyroscope data with accelerometer and magnetic field readings. The correction part of the filter is based on the method described above and works for both IMU (Inertial Measurement Unit) and MARG (Magnetic, Angular Rate, and Gravity) sensors. We evaluate the effectiveness of the filter and show that it significantly outperforms other common methods, using publicly available datasets with ground-truth data recorded during a real flight experiment of a micro quadrotor helicopter. PMID:26258778

Keeping a Good Attitude: A Quaternion-Based Orientation Filter for IMUs and MARGs.

PubMed

Valenti, Roberto G; Dryanovski, Ivan; Xiao, Jizhong

2015-08-06

Orientation estimation using low cost sensors is an important task for Micro Aerial Vehicles (MAVs) in order to obtain a good feedback for the attitude controller. The challenges come from the low accuracy and noisy data of the MicroElectroMechanical System (MEMS) technology, which is the basis of modern, miniaturized inertial sensors. In this article, we describe a novel approach to obtain an estimation of the orientation in quaternion form from the observations of gravity and magnetic field. Our approach provides a quaternion estimation as the algebraic solution of a system from inertial/magnetic observations. We separate the problems of finding the "tilt" quaternion and the heading quaternion in two sub-parts of our system. This procedure is the key for avoiding the impact of the magnetic disturbances on the roll and pitch components of the orientation when the sensor is surrounded by unwanted magnetic flux. We demonstrate the validity of our method first analytically and then empirically using simulated data. We propose a novel complementary filter for MAVs that fuses together gyroscope data with accelerometer and magnetic field readings. The correction part of the filter is based on the method described above and works for both IMU (Inertial Measurement Unit) and MARG (Magnetic, Angular Rate, and Gravity) sensors. We evaluate the effectiveness of the filter and show that it significantly outperforms other common methods, using publicly available datasets with ground-truth data recorded during a real flight experiment of a micro quadrotor helicopter.

Smelting Remains a Public Health Risk Nearly a Century Later: A Case Study in Pueblo, Colorado, USA.

PubMed

Diawara, Moussa M; Shrestha, Sofy; Carsella, Jim; Farmer, Shanna

2018-05-07

Pueblo, Colorado has a long history of smelting activities, and recent studies raised concerns about lead exposure. This study tested 240 children in Pueblo for blood lead levels (BLLs) and found a significant association between distance from old smelters and children BLLs. Around 7.5% of Pueblo children had BLLs above the Centers for Disease Control and Prevention reference level of 5 µg/dL for elevated BLL, and 18.3% had BLLs between 3.3⁻4.9 µg/dL. Out of the 36 children who lived near former smelters, 13.9% had BLLs above 5 µg/dL vs. 6.37% for children living away from old smelters. The proportion of Pueblo children with elevated BLL was nearly three times the 2007⁻2010 United States national average (7.5% vs. 2.6%), and this was higher in the immediate vicinity of old smelters (13.9% vs. 2.6%). Genetic polymorphisms for ALAD-1 or ALAD-2 alleles, which play a role in susceptibility to lead toxicity, were not associated with children BBLs. Around 38.5% of houses sampled near the smelters had topsoil lead levels higher than the Environmental Protection Agency’s benchmark of 400 mg/kg. Our study resulted in the addition of areas of Pueblo to the EPA Superfund National Priorities List in December 2014, and cleanup is currently underway to minimize the public health risks.

Micro-Cogeneration Incl. The Conversion of Chemical Energy of Biomass to Electric Energy and the Low Potential Heat

NASA Astrophysics Data System (ADS)

Huzvar, Jozef; Kapjor, Andrej

2011-06-01

This article deals with combined production of heat and electricity for small premises, such as households, where energy consumption is around few kilowatts. This proposal of micro co-generation unit uses as a heat source an automatic burner for combustion of wood pellets. Construction of an equipment for the heat transport can be designed using different basic ways of heat transfer. Electricity is produced by the two-stroke steam engine and the generator.

Study of plasma-derived miRNAs mimic differences in Huntington's disease brain.

PubMed

Hoss, Andrew G; Lagomarsino, Valentina N; Frank, Samuel; Hadzi, Tiffany C; Myers, Richard H; Latourelle, Jeanne C

2015-12-01

Biomarkers for Huntington's disease progression could accelerate therapeutic developments and improve patient care. Brain microRNAs relating to clinical features of Huntington's disease may represent a potential Huntington's disease biomarker in blood. This study was undertaken to examine candidate microRNAs in plasma to determine whether changes observed in HD brains are detectable in peripheral samples. Four microRNAs from 26 manifest Huntington's disease, four asymptomatic Huntington's disease gene carriers, and eight controls were quantified in plasma using reverse transcription quantitative polymerase chain reaction. Linear regression was used to assess microRNA levels across control, asymptomatic gene carriers, and manifest patients. miR-10b-5p (P = 0.0068) and miR-486-5p (P = 0.044) were elevated in Huntington's disease plasma. miR-10b-5p was decreased in asymptomatic gene carriers as compared with patients with Huntington's disease (P = 0.049), but no difference between asymptomatic gene carriers and healthy controls was observed (P = 0.24). These findings suggest that microRNA changes observed in Huntington's disease brain may be detectable in plasma and have potential clinical utility. © 2015 International Parkinson and Movement Disorder Society.

Functional micro/nanostructures: simple synthesis and application in sensors, fuel cells, and gene delivery.

PubMed

Guo, Shaojun; Wang, Erkang

2011-07-19

In order to develop new, high technology devices for a variety of applications, researchers would like to better control the structure and function of micro/nanomaterials through an understanding of the role of size, shape, architecture, composition, hybridization, molecular engineering, assembly, and microstructure. However, researchers continue to face great challenges in the construction of well-defined micro/nanomaterials with diverse morphologies. At the same time, the research interface where micro/nanomaterials meet electrochemistry, analytical chemistry, biomedicine, and other fields provides rich opportunities to reveal new chemical, physical, and biological properties of micro/nanomaterials and to uncover many new functions and applications of these materials. In this Account, we describe our recent progress in the construction of novel inorganic and polymer nanostructures formed through different simple strategies. Our synthetic strategies include wet-chemical and electrochemical methods for the controlled production of inorganic and polymer nanomaterials with well-defined morphologies. These methods are both facile and reliable, allowing us to produce high-quality micro/nanostructures, such as nanoplates, micro/nanoflowers, monodisperse micro/nanoparticles, nanowires, nanobelts, and polyhedron and even diverse hybrid structures. We implemented a series of approaches to address the challenges in the preparation of new functional micro/nanomaterials for a variety of important applications This Account also highlights new or enhanced applications of certain micro/nanomaterials in sensing applications. We singled out analytical techniques that take advantage of particular properties of micro/nanomaterials. Then by rationally tailoring experimental parameters, we readily and selectively obtained different types of micro/nanomaterials with novel morphologies with high performance in applications such as electrochemical sensors, electrochemiluminescent sensors, gene delivery agents, and fuel cell catalysts. We expect that micro/nanomaterials with unique structural characteristics, properties, and functions will attract increasing research interest and will lead to new opportunities in various fields of research.

A plasma microRNA signature as a biomarker for acquired aplastic anemia.

PubMed

Hosokawa, Kohei; Kajigaya, Sachiko; Feng, Xingmin; Desierto, Marie J; Fernandez Ibanez, Maria Del Pilar; Rios, Olga; Weinstein, Barbara; Scheinberg, Phillip; Townsley, Danielle M; Young, Neal S

2017-01-01

Aplastic anemia is an acquired bone marrow failure characterized by marrow hypoplasia, a paucity of hematopoietic stem and progenitor cells, and pancytopenia of the peripheral blood, due to immune attack on the bone marrow. In aplastic anemia, a major challenge is to develop immune biomarkers to monitor the disease. We measured circulating microRNAs in plasma samples of aplastic anemia patients in order to identify disease-specific microRNAs. A total of 179 microRNAs were analyzed in 35 plasma samples from 13 aplastic anemia patients, 11 myelodysplastic syndrome patients, and 11 healthy controls using the Serum/Plasma Focus microRNA Polymerase Chain Reaction Panel. Subsequently, 19 microRNAs from the discovery set were investigated in the 108 plasma samples from 41 aplastic anemia patients, 24 myelodysplastic syndrome patients, and 43 healthy controls for validation, confirming that 3 microRNAs could be validated as dysregulated (>1.5-fold change) in aplastic anemia, compared to healthy controls. MiR-150-5p (induction of T-cell differentiation) and miR-146b-5p (involvement in the feedback regulation of innate immune response) were elevated in aplastic anemia plasma, whereas miR-1 was decreased in aplastic anemia. By receiver operating characteristic curve analysis, we developed a logistic model with these 3 microRNAs that enabled us to predict the probability of a diagnosis of aplastic anemia with an area under the curve of 0.86. Dysregulated expression levels of the microRNAs became normal after immunosuppressive therapy at 6 months. Specifically, miR-150-5p expression was significantly reduced after successful immunosuppressive therapy, but did not change in non-responders. We propose 3 novel plasma biomarkers in aplastic anemia, in which miR-150-5p, miR-146b-5p, and miR-1 can serve for diagnosis and miR-150-5p for disease monitoring. Clinicaltrials.gov identifiers:00260689, 00217594, 00961064. Copyright© Ferrata Storti Foundation.

Micro-tube mass production device for microbial culture.

PubMed

Fujimoto, K; Ogawa, M; Higashi, K; Miki, N

2016-08-01

This paper describes mass production system of micro-tubes for microbial culture in an open environment. Microbes are used in many fields, such as food, medicine, environmental and energy. We proposed a microbe culture system using hydrogel micro-tubes, which can protect the target microbes inside from competitive microbes outside of the tubes while allow oxygen and nutrition to diffuse through. The hydrogel micro-tubes can be produced by a microfluidic device, which can precisely control the flow and therefore, the tube geometry. For practical applications of the micro-tube-based microbial culture, one of the biggest challenges is the scale-up of the micro-tube-based culture system, or mass production of the tubes. We developed a fluidic system that can produce multiple micro-tubes in parallel. We characterized the mass-produced micro channels and verified the effectiveness of the system.

The Role of Volunteerism on Social Integration and Adaptation of African Students at a Mid-Western University in the United States

ERIC Educational Resources Information Center

Manguvo, Angellar; Whitney, Stephen; Chareka, Ottilia

2013-01-01

This study examined the role of volunteer experiences on Black African international students' social integration and adaptation at a predominantly White Mid-Western university in the United States. The study explores micro-level interactions and relationships fostered during volunteering as well as feelings of inclusion/exclusion and personal…

Rapid presumptive identification of the Mycobacterium tuberculosis-bovis complex by radiometric determination of heat stable urease

DOE Office of Scientific and Technical Information (OSTI.GOV)

Gandy, J.H.; Pruden, E.L.; Cox, F.R.

1983-12-01

Simple and rapid Bactec methodologies for the determination of neat (unaltered) and heat stable urease activity of mycobacteria are presented. Clinical isolates (63) and stock cultures (32)--consisting of: M. tuberculosis (19), M. bovis (5), M. kansasii (15), M. marinum (4), M. simiae (3), M. scrofulaceum (16), M. gordonae (6), M. szulgai (6), M. flavescens (1), M. gastri (1), M. intracellulare (6), M. fortuitum-chelonei complex (12), and M. smegmatis (1)--were tested for neat urease activity by Bactec radiometry. Mycobacterial isolates (50-100 mg wet weight) were incubated at 35 degrees C for 30 minutes with microCi14C-urea. Urease-positive mycobacteria gave Bactec growth indexmore » (GI) values greater than 100 units, whereas urease-negative species gave values less than 10 GI units. Eighty-three isolates possessing neat urease activity were heated at 80 degrees C for 30 minutes followed by incubation at 35 degrees C for 30 minutes with 1 microCi14C-urea. Mycobacterium tuberculosis-bovis complex demonstrated heat-stable urease activity (GI more than 130 units) and could be distinguished from mycobacteria other than tuberculosis (MOTT), which gave GI values equal to or less than 40 units.« less

Influence of Micro Threads Alteration on Osseointegration and Primary Stability of Implants: An FEA and In Vivo Analysis in Rabbits.

PubMed

Chowdhary, Ramesh; Halldin, Anders; Jimbo, Ryo; Wennerberg, Ann

2015-06-01

To describe the early bone tissue response to implants with and without micro threads designed to the full length of an oxidized titanium implant. A pair of two-dimensional finite element models was designed using a computer aided three-dimensional interactive application files of an implant model with micro threads in between macro threads and one without micro threads. Oxidized titanium implants with (test implants n=20) and without (control implants n=20) micro thread were prepared. A total of 12 rabbits were used and each received four implants. Insertion torque while implant placement and removal torque analysis after 4 weeks was performed in nine rabbits, and histomorphometric analysis in three rabbits, respectively. Finite element analysis showed less stress accumulation in test implant models with 31Mpa when compared with 62.2 Mpa in control implant model. Insertion and removal torque analysis did not show any statistical significance between the two implant designs. At 4 weeks, there was a significant difference between the two groups in the percentage of new bone volume and bone-to-implant contact in the femur (p< .05); however, not in the tibia. The effect of micro threads was prominent in the femur suggesting that micro threads promote bone formation. The stress distribution supported by the micro threads was especially effective in the cancellous bone. © 2013 Wiley Periodicals, Inc.

Regulation of Vapor Pressure Deficit by Greenhouse Micro-Fog Systems Improved Growth and Productivity of Tomato via Enhancing Photosynthesis during Summer Season

PubMed Central

Zhang, Dalong; Zhang, Zhongdian; Li, Jianming; Chang, Yibo; Du, Qingjie; Pan, Tonghua

2015-01-01

The role of a proposed micro-fog system in regulating greenhouse environments and enhancing tomato (Solanum lycopersicum L.) productivity during summer season was studied. Experiments were carried out in a multi-span glass greenhouse, which was divided into two identical compartments involving different environments: (1) without environment control and (2) with a micro-fog system operating when the air vapor pressure deficit (VPD) of greenhouse was higher than 0.5 KPa. The micro-fog system effectively alleviated heat stress and evaporative demand in the greenhouse during summer season. The physiologically favourable environment maintained by micro-fog treatment significantly enhanced elongation of leaf and stem, which contributed to a substantial elevation of final leaf area and shoot biomass. These improvements in physiological and morphological traits resulted in around 12.3% increase of marketable tomato yield per plant. Relative growth rate (RGR) of micro-fog treatment was also significantly higher than control plants, which was mainly determined by the substantial elevation in net assimilation rate (NAR), and to a lesser extent caused by leaf area ratio (LAR). Measurement of leaf gas exchange parameters also demonstrated that micro-fog treatment significantly enhanced leaf photosynthesis capacity. Taken together, manipulation of VPD in greenhouses by micro-fog systems effectively enhanced tomato growth and productivity via improving photosynthesis during summer season. PMID:26221726

Turbo Pump Fed Micro-Rocket Engine

NASA Astrophysics Data System (ADS)

Miotti, P.; Tajmar, M.; Seco, F.; Guraya, C.; Perennes, F.; Soldati, A.; Lang, M.

2004-10-01

Micro-satellites (from 10kg up to 100kg) have mass, volume, and electrical power constraints due to their low dimensions. These limitations lead to the lack in currently available active orbit control systems in micro-satellites. Therefore, a micro-propulsion system with a high thrust to mass ratio is required to increase the potential functionality of small satellites. Mechatronic is presently working on a liquid bipropellant micro-rocket engine under contract with ESA (Contract No.16914/NL/Sfe - Micro-turbo-machinery Based Bipropellant System Using MNT). The advances in Mechatronic's project are to realise a micro-rocket engine with propellants pressurised by micro-pumps. The energy for driving the pumps would be extracted from a micro-turbine. Cooling channels around the nozzle would be also used in order to maintain the wall material below its maximum operating temperature. A mass budget comparison with more traditional pressure-fed micro-rockets shows a real benefit from this system in terms of mass reduction. In the paper, an overview of the project status in Mechatronic is presented.

Characterization of spiral ganglion neurons cultured on silicon micro-pillar substrates for new auditory neuro-electronic interfaces.

PubMed

Mattotti, M; Micholt, L; Braeken, D; Kovačić, D

2015-04-01

One of the strategies to improve cochlear implant technology is to increase the number of electrodes in the neuro-electronic interface. The objective was to characterize in vitro cultures of spiral ganglion neurons (SGN) cultured on surfaces of novel silicon micro-pillar substrates (MPS). SGN from P5 rat pups were cultured on MPS with different micro-pillar widths (1-5.6 μm) and spacings (0.6-15 μm) and were compared with control SGN cultures on glass coverslips by immunocytochemistry and scanning electron microscopy (SEM). Overall, MPS support SGN growth equally well as the control glass surfaces. Micro-pillars of a particular size-range (1.2-2.4 μm) were optimal in promoting SGN presence, neurite growth and alignment. On this specific micro-pillar size, more SGN were present, and neurites were longer and more aligned. SEM pictures highlight how cells on micro-pillars with smaller spacings grow directly on top of pillars, while at wider spacings (from 3.2 to 15 μm) they grow on the bottom of the surface, losing contact guidance. Further, we found that MPS encourage more monopolar and bipolar SGN morphologies compared to the control condition. Finally, MPS induce longest neurite growth with minimal interaction of S100+ glial cells. These results indicate that silicon micro-pillar substrates create a permissive environment for the growth of primary auditory neurons promoting neurite sprouting and are a promising technology for future high-density three-dimensional CMOS-based auditory neuro-electronic interfaces.

Ground control station software design for micro aerial vehicles

NASA Astrophysics Data System (ADS)

Walendziuk, Wojciech; Oldziej, Daniel; Binczyk, Dawid Przemyslaw; Slowik, Maciej

2017-08-01

This article describes the process of designing the equipment part and the software of a ground control station used for configuring and operating micro unmanned aerial vehicles (UAV). All the works were conducted on a quadrocopter model being a commonly accessible commercial construction. This article contains a characteristics of the research object, the basics of operating the micro aerial vehicles (MAV) and presents components of the ground control station model. It also describes the communication standards for the purpose of building a model of the station. Further part of the work concerns the software of the product - the GIMSO application (Generally Interactive Station for Mobile Objects), which enables the user to manage the actions and communication and control processes from the UAV. The process of creating the software and the field tests of a station model are also presented in the article.

Biomimetic patterned surfaces for controllable friction in micro- and nanoscale devices

NASA Astrophysics Data System (ADS)

Singh, Arvind; Suh, Kahp-Yang

2013-12-01

Biomimetics is the study and simulation of biological systems for desired functional properties. It involves the transformation of underlying principles discovered in nature into man-made technologies. In this context, natural surfaces have significantly inspired and motivated new solutions for micro- and nano-scale devices (e.g., Micro/Nano-Electro-Mechanical Systems, MEMS/NEMS) towards controllable friction, during their operation. As a generic solution to reduce friction at small scale, various thin films/coatings have been employed in the last few decades. In recent years, inspiration from `Lotus Effect' has initiated a new research direction for controllable friction with biomimetic patterned surfaces. By exploiting the intrinsic hydrophobicity and ability to reduce contact area, such micro- or nano-patterned surfaces have demonstrated great strength and potential for applications in MEMS/NEMS devices. This review highlights recent advancements on the design, development and performance of these biomimetic patterned surfaces. Also, we present some hybrid approaches to tackle current challenges in biomimetic tribological applications for MEMS/NEMS devices.

Applicability of New Approaches of Sensor Orientation to Micro Aerial Vehicles

NASA Astrophysics Data System (ADS)

Rehak, M.; Skaloud, J.

2016-06-01

This study highlights the benefits of precise aerial position and attitude control in the context of mapping with Micro Aerial Vehicles (MAVs). Accurate mapping with MAVs is gaining importance in applications such as corridor mapping, road and pipeline inspections or mapping of large areas with homogeneous surface structure, e.g. forests or agricultural fields. There, accurate aerial control plays a major role in successful terrain reconstruction and artifact-free ortophoto generation. The presented experiments focus on new approaches of aerial control. We confirm practically that the relative aerial position and attitude control can improve accuracy in difficult mapping scenarios. Indeed, the relative orientation method represents an attractive alternative in the context of MAVs for two reasons. First, the procedure is somewhat simplified, e.g. the angular misalignment, so called boresight, between the camera and the inertial measurement unit (IMU) does not have to be determined and, second, the effect of possible systematic errors in satellite positioning (e.g. due to multipath and/or incorrect recovery of differential carrier-phase ambiguities) is mitigated. First, we present a typical mapping project over an agricultural field and second, we perform a corridor road mapping. We evaluate the proposed methods in scenarios with and without automated image observations. We investigate a recently proposed concept where adjustment is performed using image observations limited to ground control and check points, so called fast aerial triangulation (Fast AT). In this context we show that accurate aerial control (absolute or relative) together with a few image observations can deliver accurate results comparable to classical aerial triangulation with thousands of image measurements. This procedure in turns reduces the demands on processing time and the requirements on the existence of surface texture. Finally, we compare the above mentioned procedures with direct sensor orientation (DiSO) to show its potential for rapid mapping.

Application of interleaved flyback micro inverter in a grid connected system

NASA Astrophysics Data System (ADS)

Brindha, R.; Ananthichristy, A.; Poornima, P. U.; Madhana, M.; Ashok Rathish, S.; Ragavi, Selvam

2018-04-01

The two control strategies CCM and DCM have various effects on the loss distribution and efficiency and thus were studied for the interleaved flyback micro inverter concentrating on the loss analysis under different load conditions. The dominant losses with heavy load include the conduction loss and the transformer loss in case of the interleaved flyback micro inverter; whereas driving of gate loss, the turn-off loss in the transformer core loss and in the powermosfets are included in the dominant losses with light load. A new hybrid control strategy which has the one-phase DCM and two-phase DCM control reduces the dominant losses in order to improving the efficiency based on the load in wide load range is proposed here.

Documentation of the ISA Micro Computed Tomography System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Brown, William D.; Smith, Jerel A.

2013-12-18

This document is intended to provide information on the ISA Micro Computed Tomography (MicroCT) system that will be installed in Yavne, Israel. X-ray source, detector, and motion control hardware are specified as well as specimen platforms, containers, and reference material types. Most of the details on the system are derived from Reference 1 and 2.

Terrestrial Micro Renewable Energy Applications of Space Technology

NASA Astrophysics Data System (ADS)

Komerath, N. M.; Komerath, P. P.

This paper explores the synergy between technologies intended for extraterrestrial in situ resource utilization and those for terrestrial mass-market micro renewable power generation systems. The case for a micro renewable energy architecture is presented. The obstacles hindering market success are summarized, along with opportunities from recent demonstrations suggesting that the public appetite for sophisticated technology worldwide may be underappreciated by technical researchers. Technical innovations from space research are summarized along with estimates of possible conversion efficiencies. It is argued that the cost-effectiveness of micro power generation must be viewed through the value of the first few watts of available power, rather than the marginal cost per kilowatt-hour of electric power from utility power grids. This leads to the finding that the actual target cost per unit power, and efficiency, are well within reach of space technology products. Hybrid systems integrating power extraction from multiple resources, and adaptable for multiple applications, can break through mass market price barriers. Recent work to develop learning resources and test beds as part of a Micro Renewable Energy Laboratory is summarized.

In-line photonic microcells based on the elliptical microfibers for refractive index sensors applications

NASA Astrophysics Data System (ADS)

Jin, Wa; Liu, Xuejing; Jin, Wei

2017-10-01

We report the fabrication of in-line photonic microcells (PMCs) by encapsulating tapered elliptical microfibers (MFs) inside glass tubes. The encapsulation does not change the optical property of the MF but protects the elliptical MF from external disturbance and contamination and makes the micro-laboratory robust. Such micro-laboratory can be easily integrated into standard fiber-optic circuits with low loss, making the elliptical MF-based devices more practical for real-world applications. Evanescent field sensing is realized by fabricating micro-channel on the PMC for ingress/egress of sample liquids/gas. Based on the encapsulated elliptical MF PMCs, we demonstrated RI sensitivity of 2024 nm per refractive index unit (nm/RIU) in gaseous environment and 21231 nm/RIU in water.

Investigation on the Acoustic Absorption of Flexible Micro-Perforated Panel with Ultra-Micro Perforations

NASA Astrophysics Data System (ADS)

Li, Guoxin; Tang, Xiaoning; Zhang, Xiaoxiao; Qian, Y. J.; Kong, Deyi

2017-11-01

Flexible micro-perforated panel has unique advantages in noise reduction due to its good flexibility compared with traditional rigid micro-perforated panel. In this paper, flexible micro-perforated panel was prepared by computer numerical control (CNC) milling machine. Three kinds of plastics including polyvinylchloride (PVC), polyethylene terephthalate (PET), and polyimide (PI) were taken as the matrix materials to prepare flexible micro-perforated panel. It has been found that flexible micro-perforated panel made of PET possessing good porosity and proper density, elastic modulus and poisson ratio exhibited the best acoustic absorption properties. The effects of various structural parameters including perforation diameter, perforation ratio, thickness and air gap have also been investigated, which would be helpful to the optimization of acoustic absorption properties.

Reconfigurable and responsive droplet-based compound micro-lenses.

PubMed

Nagelberg, Sara; Zarzar, Lauren D; Nicolas, Natalie; Subramanian, Kaushikaram; Kalow, Julia A; Sresht, Vishnu; Blankschtein, Daniel; Barbastathis, George; Kreysing, Moritz; Swager, Timothy M; Kolle, Mathias

2017-03-07

Micro-scale optical components play a crucial role in imaging and display technology, biosensing, beam shaping, optical switching, wavefront-analysis, and device miniaturization. Herein, we demonstrate liquid compound micro-lenses with dynamically tunable focal lengths. We employ bi-phase emulsion droplets fabricated from immiscible hydrocarbon and fluorocarbon liquids to form responsive micro-lenses that can be reconfigured to focus or scatter light, form real or virtual images, and display variable focal lengths. Experimental demonstrations of dynamic refractive control are complemented by theoretical analysis and wave-optical modelling. Additionally, we provide evidence of the micro-lenses' functionality for two potential applications-integral micro-scale imaging devices and light field display technology-thereby demonstrating both the fundamental characteristics and the promising opportunities for fluid-based dynamic refractive micro-scale compound lenses.

Reconfigurable and responsive droplet-based compound micro-lenses

PubMed Central

Nagelberg, Sara; Zarzar, Lauren D.; Nicolas, Natalie; Subramanian, Kaushikaram; Kalow, Julia A.; Sresht, Vishnu; Blankschtein, Daniel; Barbastathis, George; Kreysing, Moritz; Swager, Timothy M.; Kolle, Mathias

2017-01-01

Micro-scale optical components play a crucial role in imaging and display technology, biosensing, beam shaping, optical switching, wavefront-analysis, and device miniaturization. Herein, we demonstrate liquid compound micro-lenses with dynamically tunable focal lengths. We employ bi-phase emulsion droplets fabricated from immiscible hydrocarbon and fluorocarbon liquids to form responsive micro-lenses that can be reconfigured to focus or scatter light, form real or virtual images, and display variable focal lengths. Experimental demonstrations of dynamic refractive control are complemented by theoretical analysis and wave-optical modelling. Additionally, we provide evidence of the micro-lenses' functionality for two potential applications—integral micro-scale imaging devices and light field display technology—thereby demonstrating both the fundamental characteristics and the promising opportunities for fluid-based dynamic refractive micro-scale compound lenses. PMID:28266505

Development of fast wireless detection system for fixed offshore platform

NASA Astrophysics Data System (ADS)

Li, Zhigang; Yu, Yan; Jiao, Dong; Wang, Jie; Li, Zhirui; Ou, Jinping

2011-04-01

Offshore platforms' security is concerned since in 1950s and 1960s, and in the early 1980s some important specifications and standards are built, and all these provide technical basis of fixed platform design, construction, installation and evaluation. With the condition that more and more platforms are in serving over age, the research about the evaluation and detection technology of offshore platform has been a hotspot, especially underwater detection, and assessment method based on the finite element calculation. For fixed platform structure detection, conventional NDT methods, such as eddy current, magnetic powder, permeate, X-ray and ultrasonic, etc, are generally used. These techniques are more mature, intuitive, but underwater detection needs underwater robot, the necessary supporting tools of auxiliary equipment, and trained professional team, thus resources and cost used are considerable, installation time of test equipment is long. This project presents a new kind of fast wireless detection and damage diagnosis system for fixed offshore platform using wireless sensor networks, that is, wireless sensor nodes can be put quickly on the offshore platform, detect offshore platform structure global status by wireless communication, and then make diagnosis. This system is operated simply, suitable for offshore platform integrity states rapid assessment. The designed system consists in intelligence acquisition equipment and 8 wireless collection nodes, the whole system has 64 collection channels, namely every wireless collection node has eight 16-bit accuracy of A/D channels. Wireless collection node, integrated with vibration sensing unit, embedded low-power micro-processing unit, wireless transceiver unit, large-capacity power unit, and GPS time synchronization unit, can finish the functions such as vibration data collection, initial analysis, data storage, data wireless transmission. Intelligence acquisition equipment, integrated with high-performance computation unit, wireless transceiver unit, mobile power unit and embedded data analysis software, can totally control multi-wireless collection nodes, receive and analyze data, parameter identification. Data is transmitted at the 2.4GHz wireless communication channel, every sensing data channel in charge of data transmission is in a stable frequency band, control channel responsible for the control of power parameters is in a public frequency band. The test is initially conducted for the designed system, experimental results show that the system has good application prospects and practical value with fast arrangement, high sampling rate, high resolution, capacity of low frequency detection.

Glucose and amino acid metabolism in rat brain during sustained hypoglycemia

DOE Office of Scientific and Technical Information (OSTI.GOV)

Wong, K.L.; Tyce, G.M.

1983-04-01

The metabolism of glucose in brains during sustained hypoglycemia was studied. (U-/sup 14/C)Glucose (20 microCi) was injected into control rats, and into rats at 2.5 hr after a bolus injection of 2 units of insulin followed by a continuous infusion of 0.2 units/100 g rat/hr. This regimen of insulin injection was found to result in steady-state plasma glucose levels between 2.5 and 3.5 mumol per ml. In the brains of control rats carbon was transferred rapidly from glucose to glutamate, glutamine, gamma-aminobutyric acid and aspartate and this carbon was retained in the amino acids for at least 60 min. Inmore » the brains of hypoglycemic rats, the conversion of carbon from glucose to amino acids was increased in the first 15 min after injection. After 15 min, the specific activity of the amino acids decreased in insulin-treated rats but not in the controls. The concentrations of alanine, glutamate, and gamma-amino-butyric acid decreased, and the concentration of aspartate increased, in the brains of the hypoglycemic rats. The concentration of pyridoxal-5'-phosphate, a cofactor in many of the reactions whereby these amino acids are formed from tricarboxylic acid cycle intermediates, was less in the insulin-treated rats than in the controls. These data provide evidence that glutamate, glutamine, aspartate, and GABA can serve as energy sources in brain during insulin-induced hypoglycemia.« less

Indoor Autonomous Control of a Two-Wheeled Inverted Pendulum Vehicle Using Ultra Wide Band Technology.

PubMed

Xia, Dunzhu; Yao, Yanhong; Cheng, Limei

2017-06-15

In this paper, we aimed to achieve the indoor tracking control of a two-wheeled inverted pendulum (TWIP) vehicle. The attitude data are acquired from a low cost micro inertial measurement unit (IMU), and the ultra-wideband (UWB) technology is utilized to obtain an accurate estimation of the TWIP's position. We propose a dual-loop control method to realize the simultaneous balance and trajectory tracking control for the TWIP vehicle. A robust adaptive second-order sliding mode control (2-RASMC) method based on an improved super-twisting (STW) algorithm is investigated to obtain the control laws, followed by several simulations to verify its robustness. The outer loop controller is designed using the idea of backstepping. Moreover, three typical trajectories, including a circle, a trifolium and a hexagon, have been designed to prove the adaptability of the control combinations. Six different combinations of inner and outer loop control algorithms have been compared, and the characteristics of inner and outer loop algorithm combinations have been analyzed. Simulation results demonstrate its tracking performance and thus verify the validity of the proposed control methods. Trajectory tracking experiments in a real indoor environment have been performed using our experimental vehicle to further validate the feasibility of the proposed algorithm in practice.

Indoor Autonomous Control of a Two-Wheeled Inverted Pendulum Vehicle Using Ultra Wide Band Technology

PubMed Central

Xia, Dunzhu; Yao, Yanhong; Cheng, Limei

2017-01-01

In this paper, we aimed to achieve the indoor tracking control of a two-wheeled inverted pendulum (TWIP) vehicle. The attitude data are acquired from a low cost micro inertial measurement unit (IMU), and the ultra-wideband (UWB) technology is utilized to obtain an accurate estimation of the TWIP’s position. We propose a dual-loop control method to realize the simultaneous balance and trajectory tracking control for the TWIP vehicle. A robust adaptive second-order sliding mode control (2-RASMC) method based on an improved super-twisting (STW) algorithm is investigated to obtain the control laws, followed by several simulations to verify its robustness. The outer loop controller is designed using the idea of backstepping. Moreover, three typical trajectories, including a circle, a trifolium and a hexagon, have been designed to prove the adaptability of the control combinations. Six different combinations of inner and outer loop control algorithms have been compared, and the characteristics of inner and outer loop algorithm combinations have been analyzed. Simulation results demonstrate its tracking performance and thus verify the validity of the proposed control methods. Trajectory tracking experiments in a real indoor environment have been performed using our experimental vehicle to further validate the feasibility of the proposed algorithm in practice. PMID:28617338

The effect of two different visual presentation modalities on the narratives of mainstream grade 3 children.

PubMed

Klop, D; Engelbrecht, L

2013-12-01

This study investigated whether a dynamic visual presentation method (a soundless animated video presentation) would elicit better narratives than a static visual presentation method (a wordless picture book). Twenty mainstream grade 3 children were randomly assigned to two groups and assessed with one of the visual presentation methods. Narrative performance was measured in terms of micro- and macrostructure variables. Microstructure variables included productivity (total number of words, total number of T-units), syntactic complexity (mean length of T-unit) and lexical diversity measures (number of different words). Macrostructure variables included episodic structure in terms of goal-attempt-outcome (GAO) sequences. Both visual presentation modalities elicited narratives of similar quantity and quality in terms of the micro- and macrostructure variables that were investigated. Animation of picture stimuli did not elicit better narratives than static picture stimuli.

Plant polycistronic precursors containing non-homologous microRNAs target transcripts encoding functionally related proteins

PubMed Central

2009-01-01

Background MicroRNAs (miRNAs) are endogenous single-stranded small RNAs that regulate the expression of specific mRNAs involved in diverse biological processes. In plants, miRNAs are generally encoded as a single species in independent transcriptional units, referred to as MIRNA genes, in contrast to animal miRNAs, which are frequently clustered. Results We performed a comparative genomic analysis in three model plants (rice, poplar and Arabidopsis) and characterized miRNA clusters containing two to eight miRNA species. These clusters usually encode miRNAs of the same family and certain share a common evolutionary origin across monocot and dicot lineages. In addition, we identified miRNA clusters harboring miRNAs with unrelated sequences that are usually not evolutionarily conserved. Strikingly, non-homologous miRNAs from the same cluster were predicted to target transcripts encoding related proteins. At least four Arabidopsis non-homologous clusters were expressed as single transcriptional units. Overexpression of one of these polycistronic precursors, producing Ath-miR859 and Ath-miR774, led to the DCL1-dependent accumulation of both miRNAs and down-regulation of their different mRNA targets encoding F-box proteins. Conclusions In addition to polycistronic precursors carrying related miRNAs, plants also contain precursors allowing coordinated expression of non-homologous miRNAs to co-regulate functionally related target transcripts. This mechanism paves the way for using polycistronic MIRNA precursors as a new molecular tool for plant biologists to simultaneously control the expression of different genes. PMID:19951405

Extremely stable piezo mechanisms for the new gravitational wave observatory

NASA Astrophysics Data System (ADS)

Pijnenburg, Joep; Rijnveld, Niek; Hogenhuis, Harm

2017-11-01

Detection and observation of gravitational waves requires extreme stability in the frequency range 3e-5 Hz to 1 Hz. NGO/LISA will attain this by creating a giant interferometer in space, based on free floating proof masses in three spacecrafts. To operate NGO/LISA, the following piezo mechanisms are developed: 1. A piezo stack mechanism (Point Angle Ahead Mechanism) Due to time delay in the interferometer arms, the beam angle needs to be corrected. A mechanism rotating a mirror with a piezo stack performs this task. The critical requirements are the contribution to the optical path difference (less than 1.4 pm/√Hz) and the angular jitter (less than 8 nrad/√Hz). 2. A piezo sliding mechanism (Fiber Switching Unit Actuator) To switch from primary to the redundant laser source, a Fiber Switching Unit Actuator (FSUA) is developed. The critical requirements are the coalignment of outgoing beams of <+/-1 micro radian and <+/-1 micro meter. A redundant piezo sliding mechanism rotates a wave plate over 45 degrees. 3. A piezo stepping mechanism (In Field Pointing Mechanism) Due to seasonal orbit evolution effects, beams have to be corrected over a stroke of +/-2.5 degrees. The critical requirements are the contribution to the optical path difference (less than 3.0 pm/√Hz) and the angular jitter (less than 1 nrad/√Hz). Due to the large stroke, a piezo stepping concept was selected. Dedicated control algorithms have been implemented to achieve these challenging requirements. This paper gives description of the designs and the ongoing process of qualifying the mechanisms for space applications.

Development of micro-mirror slicer integral field unit for space-borne solar spectrographs

NASA Astrophysics Data System (ADS)

Suematsu, Yoshinori; Saito, Kosuke; Koyama, Masatsugu; Enokida, Yukiya; Okura, Yukinobu; Nakayasu, Tomoyasu; Sukegawa, Takashi

2017-12-01

We present an innovative optical design for image slicer integral field unit (IFU) and a manufacturing method that overcomes optical limitations of metallic mirrors. Our IFU consists of a micro-mirror slicer of 45 arrayed, highly narrow, flat metallic mirrors and a pseudo-pupil-mirror array of off-axis conic aspheres forming three pseudo slits of re-arranged slicer images. A prototype IFU demonstrates that the final optical quality is sufficiently high for a visible light spectrograph. Each slicer micro-mirror is 1.58 mm long and 30 μm wide with surface roughness ≤1 nm rms, and edge sharpness ≤ 0.1 μm, etc. This IFU is small size and can be implemented in a multi-slit spectrograph without any moving mechanism and fore optics, in which one slit is real and the others are pseudo slits from the IFU. The IFU mirrors were deposited by a space-qualified, protected silver coating for high reflectivity in visible and near IR wavelength regions. These properties are well suitable for space-borne spectrograph such as the future Japanese solar space mission SOLAR-C. We present the optical design, performance of prototype IFU, and space qualification tests of the silver coating.

Caries risk indicators in children with type 1 diabetes mellitus in relation to metabolic control.

PubMed

El-Tekeya, Magda; El Tantawi, Maha; Fetouh, Hend; Mowafy, Ehsan; Abo Khedr, Nashwa

2012-01-01

The purpose of this study was to investigate the interaction of caries risk indicators and metabolic control in children with type 1 diabetes mellitus. The study included 50 children with type 1 DM and 50 healthy controls, all 6 to 9 years old. Diabetic children were classified into 3 groups: well, fairly, and poorly controlled based on glycosilated hemoglobin level. Personal, family data, medical and dental history were collected. Children were examined for caries experience, plaque, and gingival condition. Saliva samples were obtained for culturing mutans streptococci, lactobacilli, and Candida, and colony forming units were counted. No significant differences existed between all groups regarding caries experience or mean log count of micro-organisms. Diabetic children differed significantly from healthy children in parental occupation and education, dental visits, oral hygiene, and plaque and gingival indices, whereas no differences were observed among children with different levels of metabolic control regarding these factors. Regression analysis identified mutans streptococci as a significant variable affecting caries experience in diabetic children. Regarding the interaction of caries risk indicators and metabolic control on caries experience in diabetic children, the only variable that showed a significant effect was mutans streptococci.

Methods for implantation of micro-wire bundles and optimization of single/multiunit recordings from human mesial temporal lobe

PubMed Central

Misra, A; Burke, JF; Ramayya, A; Jacobs, J; Sperling, MR; Moxon, KA; Kahana, MJ; Evans, JJ; Sharan, AD

2014-01-01

Objective The authors report methods developed for the implantation of micro-wire bundles into mesial temporal lobe structures and subsequent single neuron recording in epileptic patients undergoing in-patient diagnostic monitoring. This is done with the intention of lowering the perceived barriers to routine single neuron recording from deep brain structures in the clinical setting. Approach Over a 15 month period, 11 patients were implanted with platinum micro-wire bundles into mesial temporal structures. Protocols were developed for A) monitoring electrode integrity through impedance testing, B) ensuring continuous 24-7 recording, C) localizing micro-wire position and “splay” pattern and D) monitoring grounding and referencing to maintain the quality of recordings. Main Result Five common modes of failure were identified: 1) broken micro-wires from acute tensile force, 2) broken micro-wires from cyclic fatigue at stress points, 3) poor in-vivo micro-electrode separation, 4) motion artifact and 5) deteriorating ground connection and subsequent drop in common mode noise rejection. Single neurons have been observed up to 14 days post implantation and on 40% of micro-wires. Significance Long-term success requires detailed review of each implant by both the clinical and research teams to identify failure modes, and appropriate refinement of techniques while moving forward. This approach leads to reliable unit recordings without prolonging operative times, which will help increase the availability and clinical viability of human single neuron data. PMID:24608589

Optical and electrical interfacing technologies for living cell bio-chips.

PubMed

Shacham-Diamand, Y; Belkin, S; Rishpon, J; Elad, T; Melamed, S; Biran, A; Yagur-Kroll, S; Almog, R; Daniel, R; Ben-Yoav, H; Rabner, A; Vernick, S; Elman, N; Popovtzer, R

2010-06-01

Whole-cell bio-chips for functional sensing integrate living cells on miniaturized platforms made by micro-system-technologies (MST). The cells are integrated, deposited or immersed in a media which is in contact with the chip. The cells behavior is monitored via electrical, electrochemical or optical methods. In this paper we describe such whole-cell biochips where the signal is generated due to the genetic response of the cells. The solid-state platform hosts the biological component, i.e. the living cells, and integrates all the required micro-system technologies, i.e. the micro-electronics, micro-electro optics, micro-electro or magneto mechanics and micro-fluidics. The genetic response of the cells expresses proteins that generate: a. light by photo-luminescence or bioluminescence, b. electrochemical signal by interaction with a substrate, or c. change in the cell impedance. The cell response is detected by a front end unit that converts it to current or voltage amplifies and filters it. The resultant signal is analyzed and stored for further processing. In this paper we describe three examples of whole-cell bio chips, photo-luminescent, bioluminescent and electrochemical, which are based on the genetic response of genetically modified E. coli microbes integrated on a micro-fluidics MEMS platform. We describe the chip outline as well as the basic modeling scheme of such sensors. We discuss the highlights and problems of such system, from the point of view of micro-system-technology.

Collection, Measurement and Treatment of Microorganism Using Dielectrophoretic Micro Devices

NASA Astrophysics Data System (ADS)

Uchida, Satoshi

Constant monitoring of manufacturing processes has been essential in food industry because of global expansion of microbial infection. Micro-scale dielectrophoretic method is an attractive technique for direct operation and quantitative detection of bioparticles. The electrical system is capable of rapid and simple treatments corresponding to severe legal control for food safety. In this paper, newly developed techniques are reviewed for bacterial concentration, detection and sterilization using dielectrophoresis in a micro reactor. The perspective to an integrated micro device of those components is also discussed.

Hierarchical Pore-Patterned Carbon Electrodes for High-Volumetric Energy Density Micro-Supercapacitors.

PubMed

Kim, Cheolho; Moon, Jun Hyuk

2018-06-13

Micro-supercapacitors (MSCs) are attractive for applications in next-generation mobile and wearable devices and have the potential to complement or even replace lithium batteries. However, many previous MSCs have often exhibited a low volumetric energy density with high-loading electrodes because of the nonuniform pore structure of the electrodes. To address this issue, we introduced a uniform-pore carbon electrode fabricated by 3D interference lithography. Furthermore, a hierarchical pore-patterned carbon (hPC) electrode was formed by introducing a micropore by chemical etching into the macropore carbon skeleton. The hPC electrodes were applied to solid-state MSCs. We achieved a constant volumetric capacitance and a corresponding volumetric energy density for electrodes of various thicknesses. The hPC MSC reached a volumetric energy density of approximately 1.43 mW h/cm 3 . The power density of the hPC MSC was 1.69 W/cm 3 . We could control the capacitance and voltage additionally by connecting the unit MSC cells in series or parallel, and we confirmed the operation of a light-emitting diode. We believe that our pore-patterned electrodes will provide a new platform for compact but high-performance energy storage devices.

Laser beam soldering of micro-optical components

NASA Astrophysics Data System (ADS)

Eberhardt, R.

2003-05-01

MOTIVATION Ongoing miniaturisation and higher requirements within optical assemblies and the processing of temperature sensitive components demands for innovative selective joining techniques. So far adhesive bonding has primarily been used to assemble and adjust hybrid micro optical systems. However, the properties of the organic polymers used for the adhesives limit the application of these systems. In fields of telecommunication and lithography, an enhancement of existing joining techniques is necessary to improve properties like humidity resistance, laserstability, UV-stability, thermal cycle reliability and life time reliability. Against this background laser beam soldering of optical components is a reasonable joining technology alternative. Properties like: - time and area restricted energy input - energy input can be controlled by the process temperature - direct and indirect heating of the components is possible - no mechanical contact between joining tool and components give good conditions to meet the requirements on a joining technology for sensitive optical components. Additionally to the laser soldering head, for the assembly of optical components it is necessary to include positioning units to adjust the position of the components with high accuracy before joining. Furthermore, suitable measurement methods to characterize the soldered assemblies (for instance in terms of position tolerances) need to be developed.

The effect of hierarchical micro/nanosurface titanium implant on osseointegration in ovariectomized sheep.

PubMed

Xiao, J; Zhou, H; Zhao, L; Sun, Y; Guan, S; Liu, B; Kong, L

2011-06-01

Hydrofluoric etching and anodized hierarchical micro/nanotextured surface titanium implant was placed in mandibles of ovariectomized sheep for 12 weeks, and it showed improved osseointegration by resonance frequency analysis (RFA), microcomputed tomography (micro-CT) evaluation, histomorphometry, and biomechanical test. This study aimed to investigate the effects of micro/nanotextured titanium implant on osseointegration in ovariectomized (OVX) sheep. The hierarchical micro/nanotextured surface of titanium implant was fabricated by acid in 0.5% (w/v) hydrofluoric (HF) and anodized in HF acid electrolytes with a DC power of 20 V, and the machined surface implants with no treatment served as control group. The implants were placed in mandibles of OVX sheep, respectively. Twelve weeks after implantation, RFA, microcomputed tomography, histomorphometry, and biomechanical tests were applied to detect the osseointegration of the two groups. The implant stability quotient (ISQ) values, the maximum pull-out forces, and the bone-implant contact (BIC) were 65.5 ± 6.3, 490.6 ± 72.7 N, and 58.31 ± 5.79% in the micro/nanogroup and 58.3 ± 8.9, 394.5 ± 54.5 N, and 46.85 ± 5.04% in the control group, respectively. There was no significant difference between the two groups in ISQ values (p > 0.05), but in the micro/nanogroup, the maximal pull-out force and the BIC were increased significantly (p < 0.05 or p < 0.01). Micro-CT analysis showed that the bone volume ratio and the trabecular number increased significantly (p < 0.01), and the trabecular separation decreased significantly (p < 0.05) in the micro/nanogroup. Implant modification by HF acid etching and anodization to form a hierarchical micro/nanotextured surface could improve titanium implant osseointegration in OVX sheep 12 weeks after implantation.

Lethality and bioaccumulation of 4-nonylphenol in bluegill sunfish in littoral enclosures

DOE Office of Scientific and Technical Information (OSTI.GOV)

Liber, K.; Gangl, J.A.; Corry, T.D.

1999-03-01

Toxicity and bioaccumulation in bluegill sunfish (Lepomis macrochirus) of 4-nonylphenol (NP), a common precursor and degradation intermediate of certain nonionic surfactants, were evaluated in a set of 18 aquatic mesocosms (littoral enclosures) in northeastern Minnesota. Nonylphenol was applied to enclosures every 48 h over a 20-d period (11 applications) at rates of 3, 30, 100, and 300 {micro}g/L. Additional enclosures served as untreated controls. Maximum NP levels in the integrated water column 2 h after each application averaged 5 {+-} 4, 23 {+-} 11, 76 {+-} 21, and 243 {+-} 41 {micro}g/L over the 11 applications at the 3-, 30-,more » 100-, and 300-{micro}g/L treatments, respectively. Sixty-one percent of the NP dissipated from the water column within {approximately} 39 h of each application. Estimated survival of stocked juvenile bluegills was significantly reduced at the 300-{micro}g/L NP treatment. No significant effects were noted at the other NP treatments; however, the 100-{micro}g/L enclosure with the highest mean NP concentration (93 {+-} 39 {micro}g/L) within that treatment had four to nine times more dead bluegills and four to six times fewer bluegills captured at the end of the season than the other two 100-{micro}g/L enclosures (64 {+-} 23 and 71 {+-} 32 {micro}g/L) and the controls, suggesting increased mortality in this enclosure. Nonylphenol tissue concentrations in juvenile bluegills collected from enclosures treated with 3 and 30 {micro}g/L NP ranged from 0.01 to 2.94 {micro}g/g wet weight and showed a significant positive relationship with the average measured NP concentration in the water. The mean wet weight nonequilibrium NP bioaccumulation factor was 87 {+-} 124. There was no relationship between fish lipid content and NP tissue concentration.« less

Reflective practices at the Security Council: Children and armed conflict and the three United Nations

PubMed Central

Bode, Ingvild

2017-01-01

The United Nations Security Council passed its first resolution on children in armed conflict in 1999, making it one of the oldest examples of Security Council engagement with a thematic mandate and leading to the creation of a dedicated working group in 2005. Existing theoretical accounts of the Security Council cannot account for the developing substance of the children and armed conflict agenda as they are macro-oriented and focus exclusively on states. I argue that Security Council decision-making on thematic mandates is a productive process whose outcomes are created by and through practices of actors across the three United Nations: member states (the first United Nations), United Nations officials (the second United Nations) and non-governmental organizations (the third United Nations). In presenting a practice-based, micro-oriented analysis of the children and armed conflict agenda, the article aims to deliver on the empirical promise of practice theories in International Relations. I make two contributions to practice-based understandings: first, I argue that actors across the three United Nations engage in reflective practices of a strategic or tactical nature to manage, arrange or create space in Security Council decision-making. Portraying practices as reflective rather than as only based on tacit knowledge highlights how actors may creatively adapt their practices to social situations. Second, I argue that particular individuals from the three United Nations are more likely to become recognized as competent performers of practices because of their personality, understood as plural socialization experiences. This adds varied individual agency to practice theories that, despite their micro-level interests, have focused on how agency is relationally constituted. PMID:29782586

Reflective practices at the Security Council: Children and armed conflict and the three United Nations.

PubMed

Bode, Ingvild

2018-06-01

The United Nations Security Council passed its first resolution on children in armed conflict in 1999, making it one of the oldest examples of Security Council engagement with a thematic mandate and leading to the creation of a dedicated working group in 2005. Existing theoretical accounts of the Security Council cannot account for the developing substance of the children and armed conflict agenda as they are macro-oriented and focus exclusively on states. I argue that Security Council decision-making on thematic mandates is a productive process whose outcomes are created by and through practices of actors across the three United Nations: member states (the first United Nations), United Nations officials (the second United Nations) and non-governmental organizations (the third United Nations). In presenting a practice-based, micro-oriented analysis of the children and armed conflict agenda, the article aims to deliver on the empirical promise of practice theories in International Relations. I make two contributions to practice-based understandings: first, I argue that actors across the three United Nations engage in reflective practices of a strategic or tactical nature to manage, arrange or create space in Security Council decision-making. Portraying practices as reflective rather than as only based on tacit knowledge highlights how actors may creatively adapt their practices to social situations. Second, I argue that particular individuals from the three United Nations are more likely to become recognized as competent performers of practices because of their personality, understood as plural socialization experiences. This adds varied individual agency to practice theories that, despite their micro-level interests, have focused on how agency is relationally constituted.

Double-plasma enhanced carbon shield for spatial/interfacial controlled electrodes in lithium ion batteries via micro-sized silicon from wafer waste

NASA Astrophysics Data System (ADS)

Chen, Bing-Hong; Chuang, Shang-I.; Duh, Jenq-Gong

2016-11-01

Using spatial and interfacial control, the micro-sized silicon waste from wafer slurry could greatly increase its retention potential as a green resource for silicon-based anode in lithium ion batteries. Through step by step spatial and interfacial control for electrode, the cyclability of recycled waste gains potential performance from its original poor retention property. In the stages of spatial control, the electrode stabilizers of active, inactive and conductive additives were mixed into slurries for maintaining architecture and conductivity of electrode. In addition, a fusion electrode modification of interfacial control combines electrolyte additive, technique of double-plasma enhanced carbon shield (D-PECS) to convert the chemical bond states and to alter the formation of solid electrolyte interphases (SEIs) in the first cycle. The depth profiles of chemical composition from external into internal electrode illustrate that the fusion electrode modification not only forms a boundary to balance the interface between internal and external electrodes but also stabilizes the SEIs formation and soothe the expansion of micro-sized electrode. Through these effect approaches, the performance of micro-sized Si waste electrode can be boosted from its serious capacity degradation to potential retention (200 cycles, 1100 mAh/g) and better meet the requirements for facile and cost-effective in industrial production.

Study of nanometer-level precise phase-shift system used in electronic speckle shearography and phase-shift pattern interferometry

NASA Astrophysics Data System (ADS)

Jing, Chao; Liu, Zhongling; Zhou, Ge; Zhang, Yimo

2011-11-01

The nanometer-level precise phase-shift system is designed to realize the phase-shift interferometry in electronic speckle shearography pattern interferometry. The PZT is used as driving component of phase-shift system and translation component of flexure hinge is developed to realize micro displacement of non-friction and non-clearance. Closed-loop control system is designed for high-precision micro displacement, in which embedded digital control system is developed for completing control algorithm and capacitive sensor is used as feedback part for measuring micro displacement in real time. Dynamic model and control model of the nanometer-level precise phase-shift system is analyzed, and high-precision micro displacement is realized with digital PID control algorithm on this basis. It is proved with experiments that the location precision of the precise phase-shift system to step signal of displacement is less than 2nm and the location precision to continuous signal of displacement is less than 5nm, which is satisfied with the request of the electronic speckle shearography and phase-shift pattern interferometry. The stripe images of four-step phase-shift interferometry and the final phase distributed image correlated with distortion of objects are listed in this paper to prove the validity of nanometer-level precise phase-shift system.

Design and prototyping of a chip-based multi-micro-organoid culture system for substance testing, predictive to human (substance) exposure.

PubMed

Sonntag, Frank; Schilling, Niels; Mader, Katja; Gruchow, Mathias; Klotzbach, Udo; Lindner, Gerd; Horland, Reyk; Wagner, Ilka; Lauster, Roland; Howitz, Steffen; Hoffmann, Silke; Marx, Uwe

2010-07-01

Dynamic miniaturized human multi-micro-organ bioreactor systems are envisaged as a possible solution for the embarrassing gap of predictive substance testing prior to human exposure. A rational approach was applied to simulate and design dynamic long-term cultures of the smallest possible functional human organ units, human "micro-organoids", on a chip the shape of a microscope slide. Each chip contains six identical dynamic micro-bioreactors with three different micro-organoid culture segments each, a feed supply and waste reservoirs. A liver, a brain cortex and a bone marrow micro-organoid segment were designed into each bioreactor. This design was translated into a multi-layer chip prototype and a routine manufacturing procedure was established. The first series of microscopable, chemically resistant and sterilizable chip prototypes was tested for matrix compatibility and primary cell culture suitability. Sterility and long-term human cell survival could be shown. Optimizing the applied design approach and prototyping tools resulted in a time period of only 3 months for a single design and prototyping cycle. This rapid prototyping scheme now allows for fast adjustment or redesign of inaccurate architectures. The designed chip platform is thus ready to be evaluated for the establishment and maintenance of the human liver, brain cortex and bone marrow micro-organoids in a systemic microenvironment. Copyright (c) 2010 Elsevier B.V. All rights reserved.

Transient Control of Synchronous Machine Active and Reactive Power in Micro-grid Power Systems

NASA Astrophysics Data System (ADS)

Weber, Luke G.

There are two main topics associated with this dissertation. The first is to investigate phase-to-neutral fault current magnitude occurring in generators with multiple zero-sequence current sources. The second is to design, model, and tune a linear control system for operating a micro-grid in the event of a separation from the electric power system. In the former case, detailed generator, AC8B excitation system, and four-wire electric power system models are constructed. Where available, manufacturers data is used to validate the generator and exciter models. A gain-delay with frequency droop control is used to model an internal combustion engine and governor. The four wire system is connected through a transformer impedance to an infinite bus. Phase-to-neutral faults are imposed on the system, and fault magnitudes analyzed against three-phase faults to gauge their severity. In the latter case, a balanced three-phase system is assumed. The model structure from the former case - but using data for a different generator - is incorporated with a model for an energy storage device and a net load model to form a micro-grid. The primary control model for the energy storage device has a high level of detail, as does the energy storage device plant model in describing the LC filter and transformer. A gain-delay battery and inverter model is used at the front end. The net load model is intended to be the difference between renewable energy sources and load within a micro-grid system that has separated from the grid. Given the variability of both renewable generation and load, frequency and voltage stability are not guaranteed. This work is an attempt to model components of a proposed micro-grid system at the University of Wisconsin Milwaukee, and design, model, and tune a linear control system for operation in the event of a separation from the electric power system. The control module is responsible for management of frequency and active power, and voltage and reactive power. The scope of this work is to • develop a mathematical model for a salient pole, 2 damper winding synchronous generator with d axis saturation suitable for transient analysis, • develop a mathematical model for a voltage regulator and excitation system using the IEEE AC8B voltage regulator and excitation system template, • develop mathematical models for an energy storage primary control system, LC filter and transformer suitable for transient analysis, • combine the generator and energy storage models in a micro-grid context, • develop mathematical models for electric system components in the stationary abc frame and rotating dq reference frame, • develop a secondary control network for dispatch of micro-grid assets, • establish micro-grid limits of stable operation for step changes in load and power commands based on simulations of model data assuming net load on the micro-grid, and • use generator and electric system models to assess the generator current magnitude during phase-to-ground faults.

Application of in-plant control measures in some Egyptian micro-scale dairy enterprises and its impact on heavy metal contents of their products.

PubMed

Abd El-Salam, Magda Magdy; Farahat, Mohamed F; Abu-Zuid, Gaber I; Saad, Samia G

2017-09-05

Egypt is encouraging micro-scale enterprises as proved to be one of the most important reasons of economic growth. Most of the annual milk production is processed in micro-scale dairy enterprises located in squatter areas with high health risks and negative environmental impact. The aim of this study was to assess the effectiveness of in-plant control measures in controlling lead and cadmium levels in dairy products from nine Egyptian micro-scale enterprises. The results revealed that white cheese enterprises had the highest mean lead and cadmium contents; both in their raw milk (0.712 and 0.134 mg/L, respectively) and final products (0.419 and 0.061 mg/kg). Higher compliance percentages were found with cadmium levels specified in the Egyptian standards than with lead levels and ranged from 59.4% in raw milk to 100% in dry milk for cadmium levels and from 8.3% in white cheese to 66.7% in ice cream for lead; moreover, none of the collected raw milk samples were complying with the lead levels. After implementation of in-plant control measures, lower lead levels were found in all samples with reduction percentages ranging from 35.2% in raw milk from the ice cream enterprises to 73.2% in yoghurt; moreover, higher percentages of samples complied with cadmium levels. This study highlights the urgent need for applying in-plant control measures to the Egyptian micro-scale dairy enterprises to improve both safety and quality of their products.

Micro-particle transporting system using galvanotactically stimulated apo-symbiotic cells of Paramecium bursaria.

PubMed

Furukawa, Shunsuke; Karaki, Chiaki; Kawano, Tomonori

2009-01-01

It is well known that Paramecium species including green paramecia (Paramecium bursaria) migrate towards the anode when exposed to an electric field in a medium. This type of a cellular movement is known as galvanotaxis. Our previous study revealed that an electric stimulus given to P bursaria is converted to a galvanotactic cellular movement by involvement of T-type calcium channel on the plasma membrane [Aonuma et al. (2007), Z. Naturforsch. 62c, 93-102]. This phenomenon has attracted the attention of bioengineers in the fields of biorobotics or micro-robotics in order to develop electrically controllable micromachineries. Here, we demonstrate the galvanotactic controls of the cellular migration of P bursaria in capillary tubes (diameter, 1-2 mm; length, 30-240 mm). Since the Paramecium cells take up particles of various sizes, we attempted to use the electrically stimulated cells of P bursaria as the vehicle for transportation of micro-particles in the capillary system. By using apo-symbiotic cells of P bursaria obtained after forced removal of symbiotic algae, the uptake of the particles could be maximized and visualized. Then, electrically controlled transportations of particle-filled apo-symbiotic P bursaria cells were manifested. The particles transported by electrically controlled cells (varying in size from nm to /m levels) included re-introduced green algae, fluorescence-labeled polystyrene beads, magnetic microspheres, emerald green fluorescent protein (EmGFP)-labeled cells of E. coli, Indian ink, and crystals of zeolite (hydrated aluminosilicate minerals with a micro-porous structure) and some metal oxides. Since the above demonstrations were successful, we concluded that P bursaria has a potential to be employed as one of the micro-biorobotic devices used in BioMEMS (biological micro-electro-mechanical systems).

Disrupted trabecular bone micro-architecture in middle-aged male HIV-infected treated patients.

PubMed

Sellier, P; Ostertag, A; Collet, C; Trout, H; Champion, K; Fernandez, S; Lopes, A; Morgand, M; Clevenbergh, P; Evans, J; Souak, S; de Vernejoul, M-C; Bergmann, J-F

2016-08-01

HIV-infected individuals are at increased risk of incident fractures. Evaluation of trabecular bone micro-architecture is an important tool to assess bone strength, but its use has not yet been reported in middle-aged HIV-infected male individuals. The aim of the study was to compare bone micro-architecture between HIV-infected and HIV-uninfected men. In this cross-sectional study, 53 HIV-infected male individuals with a mean (± standard deviation) age of 49 ± 9 years who had been receiving antiretroviral therapy including tenofovir disoproxil fumarate (DF) for at least 60 months were compared with 50 HIV-uninfected male controls, matched for age and ethnic origin. We studied the volumetric bone density and micro-architecture of the radius and tibia using high-resolution peripheral quantitative computed tomography (HR-p QCT). Volumetric trabecular bone density was 17% lower in the tibia (P < 10(-4) ) and 16% lower in the radius (P < 10(-3) ) in HIV-infected patients compared with controls. By contrast, the cortical bone density was normal at both sites. The tibial trabecular micro-architecture differed markedly between patients and controls: bone volume/total volume (BV/TV) and trabecular number were each 13% lower (P < 10(-4) for both). Trabecular separation and inhomogeneity of the network were 18% and 24% higher in HIV-infected patients than in controls, respectively. The radial BV/TV and trabecular thickness were each 13% lower (P < 10(-3) and 10(-2) , respectively). Cortical thickness was not different between the two groups. The findings of lower volumetric trabecular bone density and disrupted trabecular micro-architectural parameters in middle-aged male HIV-infected treated patients help to explain bone frailty in these patients. © 2016 British HIV Association.

Development of data communication system with ultra high frequency radio wave for implantable artificial hearts.

PubMed

Tsujimura, Shinichi; Yamagishi, Hiroto; Sankai, Yoshiyuki

2009-01-01

In order to minimize infection risks of patients with artificial hearts, wireless data transmission methods with electromagnetic induction or light have been developed. However, these methods tend to become difficult to transmit data if the external data transmission unit moves from its proper position. To resolve this serious problem, the purpose of this study is to develop a prototype wireless data communication system with ultra high frequency radio wave and confirm its performance. Due to its high-speed communication rate, low power consumption, high tolerance to electromagnetic disturbances, and secure wireless communication, we adopted Bluetooth radio wave technology for our system. The system consists of an internal data transmission unit and an external data transmission unit (53 by 64 by 16 mm, each), and each has a Bluetooth module (radio field intensity: 4 dBm, receiver sensitivity: -80 dBm). The internal unit also has a micro controller with an 8-channel 10-bit A/D converter, and the external unit also has a RS-232C converter. We experimented with the internal unit implanted into pig meat, and carried out data transmission tests to evaluate the performance of this system in tissue thickness of up to 3 mm. As a result, data transfer speeds of about 20 kbps were achieved within the communication distance of 10 m. In conclusion, we confirmed that the system can wirelessly transmit the data from the inside of the body to the outside, and it promises to resolve unstable data transmission due to accidental movements of an external data transmission unit.

MicroRNA network changes in the brain stem underlie the development of hypertension.

PubMed

DeCicco, Danielle; Zhu, Haisun; Brureau, Anthony; Schwaber, James S; Vadigepalli, Rajanikanth

2015-09-01

Hypertension is a major chronic disease whose molecular mechanisms remain poorly understood. We compared neuroanatomical patterns of microRNAs in the brain stem of the spontaneous hypertensive rat (SHR) to the Wistar Kyoto rat (WKY, control). We quantified 419 well-annotated microRNAs in the nucleus of the solitary tract (NTS) and rostral ventrolateral medulla (RVLM), from SHR and WKY rats, during three main stages of hypertension development. Changes in microRNA expression were stage- and region-dependent, with a majority of SHR vs. WKY differential expression occurring at the hypertension onset stage in NTS versus at the prehypertension stage in RVLM. Our analysis identified 24 microRNAs showing time-dependent differential expression in SHR compared with WKY in at least one brain region. We predicted potential gene regulatory targets corresponding to catecholaminergic processes, neuroinflammation, and neuromodulation using the miRWALK and RNA22 databases, and we tested those bioinformatics predictions using high-throughput quantitative PCR to evaluate correlations of differential expression between the microRNAs and their predicted gene targets. We found a novel regulatory network motif consisting of microRNAs likely downregulating a negative regulator of prohypertensive processes such as angiotensin II signaling and leukotriene-based inflammation. Our results provide new evidence on the dynamics of microRNA expression in the development of hypertension and predictions of microRNA-mediated regulatory networks playing a region-dependent role in potentially altering brain-stem cardiovascular control circuit function leading to the development of hypertension. Copyright © 2015 the American Physiological Society.

The molecular mechanism of serum microRNA124b induced coronary heart disease by inducing myocardial cell senescence.

PubMed

Guo, M-L; Guo, L-L; Qin, Q-J; Weng, Y-Q; Wang, Y-N; Yao, J; Wang, Y-B; Zhang, X-Z; Ge, Z-M

2018-04-01

The incidence and mortality of coronary heart disease are rapidly increasing in recent years. Myocardial cell dysfunction and cell senescence may play a role in coronary heart disease. MicroRNA controls a variety of biological processes, but leaving its role in coronary heart disease has yet to be explored. Patients with coronary heart disease were regarded as subjects, and healthy volunteers as the control, on both of which microRNA124b level of serum was studied by Real-time PCR, and the heart function of patients was detected by using ultrasound. The relationship between serum microRNA124b level and cardiac function was analyzed along with the model of rat coronary artery disease; the level of aging proteins P21 and P53 in cardiac muscle cells was also tested. MicroRNA124b in the serum of patients with coronary heart disease was increased, and the heart function of patients was decreased (p < 0.05). Serum level of microRNA124b in a rat model of coronary heart disease was increased, and the cardiac function was decreased (p < 0.05). When myocardial cell appeared ageing, the level of P21 and P53 was increased, and the level of microRNA124b was related with P53. The level of microRNA124b in the serum of coronary heart disease patients and rat model may be related to the occurrence of coronary heart disease; microRNA124b may lead to the occurrence of coronary heart disease by causing cell senescence.

A compact control system to achieve stable voltage and low jitter trigger for repetitive intense electron-beam accelerator based on resonant charging

NASA Astrophysics Data System (ADS)

Qiu, Yongfeng; Liu, Jinliang; Yang, Jianhua; Cheng, Xinbing; Yang, Xiao

2017-08-01

A compact control system based on Delphi and Field Programmable Gate Array(FPGA) is developed for a repetitive intense electron-beam accelerator(IEBA), whose output power is 10GW and pulse duration is 160ns. The system uses both hardware and software solutions. It comprises a host computer, a communication module and a main control unit. A device independent applications programming interface, devised using Delphi, is installed on the host computer. Stability theory of voltage in repetitive mode is analyzed and a detailed overview of the hardware and software configuration is presented. High voltage experiment showed that the control system fulfilled the requests of remote operation and data-acquisition. The control system based on a time-sequence control method is used to keep constant of the voltage of the primary capacitor in every shot, which ensured the stable and reliable operation of the electron beam accelerator in the repetitive mode during the experiment. Compared with the former control system based on Labview and PIC micro-controller developed in our laboratory, the present one is more compact, and with higher precision in the time dimension. It is particularly useful for automatic control of IEBA in the high power microwave effects research experiments where pulse-to-pulse reproducibility is required.

Evaluation Methods Used during the Assessment of an Academic Program at Micro-Level

ERIC Educational Resources Information Center

Chinembiri, France

2017-01-01

This paper majors on the evaluation criteria and methods used for the assessment of health education programs in the United States. The choice of the topic is dictated by the fact that there is need to improve on the quality of the graduates that the different nursing and medical institutions in the United States produce. By looking at the…

Revisited study of fluorine implantation impact on negative bias temperature instability for input/output device of automotive micro controller unit

NASA Astrophysics Data System (ADS)

Yoshida, Tetsuya; Maekawa, Keiichi; Tsuda, Shibun; Shimizu, Tatsuo; Ogasawara, Makoto; Aono, Hideki; Yamaguchi, Yasuo

2018-04-01

We investigate the effect of fluorine implanted in the polycrystalline silicon (poly-Si) gate and source/drain (S/D) region on negative bias temperature instability (NBTI) improvement. It is found that there is a trade-off implantation energy dependence of NBTI between fluorine in the poly-Si gate and that in the S/D region. Fluorine implanted in the poly-Si gate contributes to NBTI improvement under low energy implantation. On the other hand, NBTI is improved by fluorine implanted in the S/D region under high energy. We propose that the two-step implantation process with high and low energy is the optimum condition for NBTI improvement.

A thermally self-sustained micro-power plant with integrated micro-solid oxide fuel cells, micro-reformer and functional micro-fluidic carrier

NASA Astrophysics Data System (ADS)

Scherrer, Barbara; Evans, Anna; Santis-Alvarez, Alejandro J.; Jiang, Bo; Martynczuk, Julia; Galinski, Henning; Nabavi, Majid; Prestat, Michel; Tölke, René; Bieberle-Hütter, Anja; Poulikakos, Dimos; Muralt, Paul; Niedermann, Philippe; Dommann, Alex; Maeder, Thomas; Heeb, Peter; Straessle, Valentin; Muller, Claude; Gauckler, Ludwig J.

2014-07-01

Low temperature micro-solid oxide fuel cell (micro-SOFC) systems are an attractive alternative power source for small-size portable electronic devices due to their high energy efficiency and density. Here, we report on a thermally self-sustainable reformer-micro-SOFC assembly. The device consists of a micro-reformer bonded to a silicon chip containing 30 micro-SOFC membranes and a functional glass carrier with gas channels and screen-printed heaters for start-up. Thermal independence of the device from the externally powered heater is achieved by exothermic reforming reactions above 470 °C. The reforming reaction and the fuel gas flow rate of the n-butane/air gas mixture controls the operation temperature and gas composition on the micro-SOFC membrane. In the temperature range between 505 °C and 570 °C, the gas composition after the micro-reformer consists of 12 vol.% to 28 vol.% H2. An open-circuit voltage of 1.0 V and maximum power density of 47 mW cm-2 at 565 °C is achieved with the on-chip produced hydrogen at the micro-SOFC membranes.

Intra-Platform Repeatability and Inter-Platform Comparability of MicroRNA Microarray Technology

PubMed Central

Sato, Fumiaki; Tsuchiya, Soken; Terasawa, Kazuya; Tsujimoto, Gozoh

2009-01-01

Over the last decade, DNA microarray technology has provided a great contribution to the life sciences. The MicroArray Quality Control (MAQC) project demonstrated the way to analyze the expression microarray. Recently, microarray technology has been utilized to analyze a comprehensive microRNA expression profiling. Currently, several platforms of microRNA microarray chips are commercially available. Thus, we compared repeatability and comparability of five different microRNA microarray platforms (Agilent, Ambion, Exiqon, Invitrogen and Toray) using 309 microRNAs probes, and the Taqman microRNA system using 142 microRNA probes. This study demonstrated that microRNA microarray has high intra-platform repeatability and comparability to quantitative RT-PCR of microRNA. Among the five platforms, Agilent and Toray array showed relatively better performances than the others. However, the current lineup of commercially available microRNA microarray systems fails to show good inter-platform concordance, probably because of lack of an adequate normalization method and severe divergence in stringency of detection call criteria between different platforms. This study provided the basic information about the performance and the problems specific to the current microRNA microarray systems. PMID:19436744

Composition and maturity of the 60013/14 core

NASA Technical Reports Server (NTRS)

Korotev, Randy L.; Morris, Richard V.; Lauer, Howard V., Jr.

1993-01-01

The 60013/14 double drive tube (62 cm deep) is one of three regolith cores taken 35-40 m apart in a triangular array on the Cayley plains at station 10' (LM/ALSEP), Apollo 16. This trio, which includes double drive tube 60009/10 (59 cm deep) and deep drill core 60001-7 (220 cm), is the only such array of cores returned from the Moon. The top 45 cm of 60013/14 is mature, as is surface reference soil 60601 taken nearby. Maturity generally decreases with depth, with soil below 45 cm being submature. The zone of lowest maturity (34 is less than or equal to I(sub s)/FeO is less than 50) extends from 46 to 58 cm depth, and corresponds to the distinct region of light-colored soil observed during core processing. In the other two cores, most of the compositional variation results from mixing between fine-grained, mature soil with 10-11 micro-g/g Sc and coarse-grained ferroan anorthosite consisting of greater than 99% plagioclase with less than 0.5 micro-g/g Sc. This is most evident in 60009/10 which contains a high abundance of plagioclase at about 54 cm depth (minimum Sc: 3-4 micro-g/g); a similar zone occurs in 60001-7 at 17-22 cm (MPU-C), although it is not as rich in plagioclase (minimum Sc: 6-7 micro-g/g). Compositional variations are less in 60013/14 than in the other two cores (range: 7.9-10.0 micro-g/g Sc), but are generally consistent with the 'plagioclase dilution' effect seen in 60009/10, i.e., most 60013/14 samples plot along the mixing line of 60009/10. However, a plagioclase component is not the cause of the lower maturity and lighter color of the unit at 46-58 cm depth in 60013/14. Many of the samples in this zone have distinctly lower Sm/Sc ratios than typical LM-area soils and plot off the mixing trend defined by 60009/10. This requires a component with moderately high Sc, but low-Sm/Sc, such as feldspathic fragmental breccia (FFB) or granulitic breccia. A component of Descartes regolith, such as occurs at North Ray Crater (NRC) and which is rich in FFB, could account for the composition of these soils (i.e., a 3:1 mixture of 60601 and NRC soil). It seems unlikely that NRC ejecta would occur half a meter deep at the LM station, thus this low-Sm/Sc component may result from an older, local crater that penetrated the Cayley surface layer and excavated underlying Descartes material, as did North Ray Crater. There is no evidence for such a unit or component in the other two cores. Soil below the light-colored unit (58-62) cm has 'typical' Sm/Sc ratios, but the lowest absolute Sc concentrations, i.e., it is compositionally equivalent to a mixture of surface soil and plagioclase such as that in ferroan anorthosite. This is the only soil that might be related to the plagioclase-rich units in the other two cores. Except for the mature soil at the top of each core and, perhaps, the plagioclase-rich layers, there is little compositional evidence for any common unit among the three cores. Soil corresponding to the mare-glass-bearing unit (MPU-B) and regolith-breccia-bearing unit (MPU-A) of 60001-7 do not occur in 60013/14 or 60009/10.

Uranium and radium concentrations in plants growing on uranium mill tailings in South Dakota

Treesearch

Mark A. Rumble; Ardell J. Bjugstad

1986-01-01

Vegetation and soil samples were collected from a uranium mill tailings site and control sites in South Dakota. Uranium concentrations in soils from the mill tailings averaged 13.3 [micro]g g-1 compared to 5.1 [micro]g g-1 in soils from control sites. 226Ra concentrations in soils averaged 111.0 pCi g...

Establishment of Rio Grande cottonwood seedlings using micro-irrigation of xeric flood plain sites

Treesearch

David R. Dreesen; Gregory A. Fenchel; Joseph G. Fraser

1999-01-01

Flood control, irrigation structures, and flow control practices on the Middle Rio Grande have prevented the deposition of sediments and hydrologic conditions conducive to the germination and establishment of Rio Grande cottonwood (Populus fremontii S. Wats.). The Los Lunas Plant Materials Center has been investigating the use of micro-irrigation systems on xeric flood...

Coordinating a Team of Robots for Urban Reconnaisance

DTIC Science & Technology

2010-11-01

Land Warfare Conference 2010 Brisbane November 2010 Coordinating a Team of Robots for Urban Reconnaisance Pradeep Ranganathan , Ryan...without inundating him with micro- management . Behavorial autonomy is also critical for the human operator to productively interact Figure 1: A...today’s systems, a human operator controls a single robot, micro- managing every action. This micro- management becomes impossible with more robots: in

Reliability of a Cryoscopic Micro-Osmometer Using 15-µL Plasma Samples to Measure Hydration Status in Varied Environmental Conditions

ERIC Educational Resources Information Center

Scanlan, Aaron T.; Richter-Stretton, Gina L.; Madueno, Maria C.; Borges, Nattai R.; Fenning, Andrew S.

2017-01-01

Measurement of plasma osmolality (P[subscript osm]) remains popular for assessing hydration status in exercise science. However, a controlled reliability assessment of micro-osmometry using small sample volumes to measure Posm remains to be performed. This study aimed to examine the reliability of a cryoscopic micro-osmometer requiring 15-µL…

Centrifuge Facility for the International Space Station Alpha

NASA Technical Reports Server (NTRS)

Johnson, Catherine C.; Hargens, Alan R.

1994-01-01

The Centrifuge Facility planned for the International Space Station Alpha has under-one considerable redesign over the past year, primarily because the Station is now viewed as a 10 year mission rather than a 30 year mission and because of the need to simply the design to meet budget constraints and a 2000 launch date. The basic elements of the Centrifuge Facility remain the same, i.e., a 2.5 m diameter centrifuge, a micro-g holding unit, plant and animal habitats, a glovebox and a service unit. The centrifuge will still provide the full range of artificial gravity from 0.01 a to 2 - as originally planned; however, the extractor to permit withdrawal of habitats from the centrifuge without stopping the centrifuge has been eliminated. The specimen habitats have also been simplified and are derived from other NASA programs. The Plant Research Unit being developed by the Gravitational Biology Facility will be used to house plants in the Centrifuge Facility. Although not as ambitious as the Centrifuge Facility plant habitat, it will provide much better environmental control and lighting than the current Shuttle based Plant Growth Facility. Similarly, rodents will be housed in the Advanced Animal Habitat being developed for the Shuttle program. The Centrifuge Facility and ISSA will provide the opportunity to perform repeatable, high quality science. The long duration increments available on the Station will permit multigeneration studies on both plants and animals which have not previously been possible. The Centrifuge Facility will accommodate sufficient number of specimens to permit statistically significant sampling of specimens to investigate the time course of adaptation to altered gravity environments. The centrifuge will for the first time permit investigators to use gravity itself as a tool to investigate fundamental processes, to investigate the intensity and duration of gravity to maintain normal structure and function, to separate the effects of micro-g from other 0 environmental factors and to examine artificial gravity as a potential countermeasure for the physical deconditioning observed during spaceflight.

Influence of a change in helical twisting power of photoresponsive chiral dopants on rotational manipulation of micro-objects on the surface of chiral nematic liquid crystalline films.

PubMed

Thomas, Reji; Yoshida, Yohei; Akasaka, Takehito; Tamaoki, Nobuyuki

2012-09-24

Herein we report a group of five planar chiral molecules as photon-mode chiral switches for the reversible control of the self-assembled superstructures of doped chiral nematic liquid crystals. The chiral switches are composed of an asymmetrically substituted aromatic moiety and a photoisomerizing azobenzene unit connected in a cyclic manner through methylene spacers of varying lengths. All the molecules show conformational restriction in the rotation of the asymmetrically substituted aromatic moiety in both the E and Z states of the azobenzene units resulting in planar chirality with separable enantiomers. Our newly synthesized compounds in pure enantiomeric form show high helical twisting power (HTP) in addition to an improved change in HTP between the E and Z states. The molecule with a diphenylnaphthalene unit shows the highest ever known initial helical twisting power among chiral dopants with planar chirality. In addition to the reversible tuning of reflection colors, we employed the enantiomers of these five compounds in combination with four nematic liquid crystalline hosts to study their properties as molecular machines; the change in HTP of the chiral dopant upon photoisomerization induces rotation of the texture of the liquid crystal surfaces. Importantly, this study has revealed a linear dependence of the ratio of the difference between HTPs before and after irradiation against the absolute value of the initial HTP, not the absolute value of the change in helical twisting power between two states, on the angle of rotation of micro-objects on chiral nematic liquid crystalline films. This study has also revealed that a change in irradiation intensity does not affect the maximum angle of rotation, but it does affect the speed of rotational reorganization of the cholesteric helix. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Micro-valve using induced-charge electrokinetic motion of Janus particle.

PubMed

Daghighi, Yasaman; Li, Dongqing

2011-09-07

A new micro-valve using the electrokinetic motion of a Janus particle is introduced in this paper. A Janus particle with a conducting hemisphere and a non-conducting hemisphere is placed in a junction of several microchannels. Under an applied electric field, the induced-charge electrokinetic flow around the conducting side of the Janus particle forms vortices. The vortices push the particle moving forwards to block the entrance of a microchannel. By switching the direction of the applied electric field, the motion of the Janus particle can be changed to block different microchannels. This paper develops a theoretical model and conducts numerical simulations of the three-dimensional transient motion of the Janus particle. The results show that this Janus particle-based micro-valve is feasible for switching and controlling the flow rate in a microfluidic chip. This method is simple in comparison with other types of micro-valve methods. It is easy for fabrication, for operation control, and has a fast response time. To better understand the micro-valve functions, comparisons with a non-conducting particle and a fully conducting particle were made. Results proved that only a Janus particle can fulfill the requirements of such a micro-valve.

Direct adaptive control of a PUMA 560 industrial robot

NASA Technical Reports Server (NTRS)

Seraji, Homayoun; Lee, Thomas; Delpech, Michel

1989-01-01

The implementation and experimental validation of a new direct adaptive control scheme on a PUMA 560 industrial robot is described. The testbed facility consists of a Unimation PUMA 560 six-jointed robot and controller, and a DEC MicroVAX II computer which hosts the Robot Control C Library software. The control algorithm is implemented on the MicroVAX which acts as a digital controller for the PUMA robot, and the Unimation controller is effectively bypassed and used merely as an I/O device to interface the MicroVAX to the joint motors. The control algorithm for each robot joint consists of an auxiliary signal generated by a constant-gain Proportional plus Integral plus Derivative (PID) controller, and an adaptive position-velocity (PD) feedback controller with adjustable gains. The adaptive independent joint controllers compensate for the inter-joint couplings and achieve accurate trajectory tracking without the need for the complex dynamic model and parameter values of the robot. Extensive experimental results on PUMA joint control are presented to confirm the feasibility of the proposed scheme, in spite of strong interactions between joint motions. Experimental results validate the capabilities of the proposed control scheme. The control scheme is extremely simple and computationally very fast for concurrent processing with high sampling rates.

Integrated model reference adaptive control and time-varying angular rate estimation for micro-machined gyroscopes

NASA Astrophysics Data System (ADS)

Tsai, Nan-Chyuan; Sue, Chung-Yang

2010-02-01

Owing to the imposed but undesired accelerations such as quadrature error and cross-axis perturbation, the micro-machined gyroscope would not be unconditionally retained at resonant mode. Once the preset resonance is not sustained, the performance of the micro-gyroscope is accordingly degraded. In this article, a direct model reference adaptive control loop which is integrated with a modified disturbance estimating observer (MDEO) is proposed to guarantee the resonant oscillations at drive mode and counterbalance the undesired disturbance mainly caused by quadrature error and cross-axis perturbation. The parameters of controller are on-line innovated by the dynamic error between the MDEO output and expected response. In addition, Lyapunov stability theory is employed to examine the stability of the closed-loop control system. Finally, the efficacy of numerical evaluation on the exerted time-varying angular rate, which is to be detected and measured by the gyroscope, is verified by intensive simulations.

The MITy micro-rover: Sensing, control, and operation

NASA Technical Reports Server (NTRS)

Malafeew, Eric; Kaliardos, William

1994-01-01

The sensory, control, and operation systems of the 'MITy' Mars micro-rover are discussed. It is shown that the customized sun tracker and laser rangefinder provide internal, autonomous dead reckoning and hazard detection in unstructured environments. The micro-rover consists of three articulated platforms with sensing, processing and payload subsystems connected by a dual spring suspension system. A reactive obstacle avoidance routine makes intelligent use of robot-centered laser information to maneuver through cluttered environments. The hazard sensors include a rangefinder, inclinometers, proximity sensors and collision sensors. A 486/66 laptop computer runs the graphical user interface and programming environment. A graphical window displays robot telemetry in real time and a small TV/VCR is used for real time supervisory control. Guidance, navigation, and control routines work in conjunction with the mapping and obstacle avoidance functions to provide heading and speed commands that maneuver the robot around obstacles and towards the target.

Controlling nitrogen migration through micro-nano networks

NASA Astrophysics Data System (ADS)

Cai, Dongqing; Wu, Zhengyan; Jiang, Jiang; Wu, Yuejin; Feng, Huiyun; Brown, Ian G.; Chu, Paul K.; Yu, Zengliang

2014-01-01

Nitrogen fertilizer unabsorbed by crops eventually discharges into the environment through runoff, leaching and volatilization, resulting in three-dimensional (3D) pollution spanning from underground into space. Here we describe an approach for controlling nitrogen loss, developed using loss control fertilizer (LCF) prepared by adding modified natural nanoclay (attapulgite) to traditional fertilizer. In the aqueous phase, LCF self-assembles to form 3D micro/nano networks via hydrogen bonds and other weak interactions, obtaining a higher nitrogen spatial scale so that it is retained by a soil filtering layer. Thus nitrogen loss is reduced and sufficient nutrition for crops is supplied, while the pollution risk of the fertilizer is substantially lowered. As such, self-fabrication of nano-material was used to manipulate the nitrogen spatial scale, which provides a novel and promising approach for the research and control of the migration of other micro-scaled pollutants in environmental medium.

Controlling nitrogen migration through micro-nano networks.

PubMed

Cai, Dongqing; Wu, Zhengyan; Jiang, Jiang; Wu, Yuejin; Feng, Huiyun; Brown, Ian G; Chu, Paul K; Yu, Zengliang

2014-01-14

Nitrogen fertilizer unabsorbed by crops eventually discharges into the environment through runoff, leaching and volatilization, resulting in three-dimensional (3D) pollution spanning from underground into space. Here we describe an approach for controlling nitrogen loss, developed using loss control fertilizer (LCF) prepared by adding modified natural nanoclay (attapulgite) to traditional fertilizer. In the aqueous phase, LCF self-assembles to form 3D micro/nano networks via hydrogen bonds and other weak interactions, obtaining a higher nitrogen spatial scale so that it is retained by a soil filtering layer. Thus nitrogen loss is reduced and sufficient nutrition for crops is supplied, while the pollution risk of the fertilizer is substantially lowered. As such, self-fabrication of nano-material was used to manipulate the nitrogen spatial scale, which provides a novel and promising approach for the research and control of the migration of other micro-scaled pollutants in environmental medium.

Controlling nitrogen migration through micro-nano networks

PubMed Central

Cai, Dongqing; Wu, Zhengyan; Jiang, Jiang; Wu, Yuejin; Feng, Huiyun; Brown, Ian G.; Chu, Paul K.; Yu, Zengliang

2014-01-01

Nitrogen fertilizer unabsorbed by crops eventually discharges into the environment through runoff, leaching and volatilization, resulting in three-dimensional (3D) pollution spanning from underground into space. Here we describe an approach for controlling nitrogen loss, developed using loss control fertilizer (LCF) prepared by adding modified natural nanoclay (attapulgite) to traditional fertilizer. In the aqueous phase, LCF self-assembles to form 3D micro/nano networks via hydrogen bonds and other weak interactions, obtaining a higher nitrogen spatial scale so that it is retained by a soil filtering layer. Thus nitrogen loss is reduced and sufficient nutrition for crops is supplied, while the pollution risk of the fertilizer is substantially lowered. As such, self-fabrication of nano-material was used to manipulate the nitrogen spatial scale, which provides a novel and promising approach for the research and control of the migration of other micro-scaled pollutants in environmental medium. PMID:24419037

Circulating miR-132-3p as a Candidate Diagnostic Biomarker for Malignant Mesothelioma

PubMed Central

Gawrych, Katarzyna; Casjens, Swaantje; Brik, Alexander; Lehnert, Martin; Taeger, Dirk; Pesch, Beate; Kollmeier, Jens; Bauer, Torsten T.; Johnen, Georg; Brüning, Thomas

2017-01-01

The use of circulating microRNAs as biomarkers has opened new opportunities for diagnosis of cancer because microRNAs exhibit tumor-specific expression profiles. The aim of this study was the identification of circulating microRNAs in human plasma as potential biomarkers for the diagnosis of malignant mesothelioma. For discovery, TaqMan Low Density Array Human MicroRNA Cards were used to analyze 377 microRNAs in plasma samples from 21 mesothelioma patients and 21 asbestos-exposed controls. For verification, individual TaqMan microRNA assays were used for quantitative real-time PCR in plasma samples from 22 mesothelioma patients and 44 asbestos-exposed controls. The circulating miR-132-3p showed different expression levels between mesothelioma patients and asbestos-exposed controls. For discrimination, sensitivity of 86% and specificity of 61% were calculated. Circulating miR-132-3p in plasma was not affected by hemolysis and no impact of age or smoking status on miR-132-3p levels could be observed. For the combination of miR-132-3p with the previously described miR-126, sensitivity of 77% and specificity of 86% were calculated. The results of this study indicate that miR-132-3p might be a new promising diagnostic biomarker for malignant mesothelioma. It is indicated that the combination of miR-132-3p with other individual biomarkers improves the biomarker performance. PMID:28321148

Multiplex fluorescent immunoassay device based on magnetic nanoparticles

NASA Astrophysics Data System (ADS)

Godjevargova, T. I.; Ivanov, Y. L.; Dinev, D. D.

2017-02-01

Immunofluorescent analyzer based compact disc for simultaneous detection of 3 antibiotics in the same milk sample is consisting of two parts: CD-based immunofluorescence kit and optoelectronic fluorometer. Kit consists of 2 parts: Lyophilized immobilized antibodies on supermagnetic nanoparticles in Eppendorf tubes and CD-based microfluidic disk, in which are formed five chamber systems for simultaneous detecting of 5 separate samples. Each system consists of 2 chambers connected by a special micro channel acting as a hydrophobic valve. In the first chamber lyophilised conjugates of 3 antibiotics with accordingly 3 different fluorescent dyes are placed. The second chamber is for detection of fluorescent signal. The optoelectronic fluorometer is comprising of: integrated thermostatic block; mechanical-detecting unit (fluorometer) and block with controlling and visualizing electronics.The disc gets into a second block of the analyzer, where centrifugation is performed and also reporting of the fluorescent signals. This unit comprises a rotor on which the disc is fixed, permanent electromagnet in the form of a ring inserted under the disc and module of 3 LED diodes with emission filters for the relevant wavelengths corresponding to the used fluorescent dyes and 1 integrated photodiode, in front of which is mounted filter with 3 spectral peaks.The signal from the photodiode is detected by the electronic unit which is sensitive "lock-in" amplifier, the engine rotor management, control of thermostatic device and management of periphery of the analyzer, consisting of display and communications with computer.

Magnetic resonant wireless power transfer for propulsion of implantable micro-robot

NASA Astrophysics Data System (ADS)

Kim, D.; Kim, M.; Yoo, J.; Park, H.-H.; Ahn, S.

2015-05-01

Recently, various types of mobile micro-robots have been proposed for medical and industrial applications. Especially in medical applications, a motor system for propulsion cannot easily be used in a micro-robot due to their small size. Therefore, micro-robots are usually actuated by controlling the magnitude and direction of an external magnetic field. However, for micro-robots, these methods in general are only applicable for moving and drilling operations, but not for the undertaking of various missions. In this paper, we propose a new micro-robot concept, which uses wireless power transfer to deliver the propulsion force and electric power simultaneously. The mechanism of Lorentz force generation and the coil design methodologies are explained, and validation of the proposed propulsion system for a micro-robot is discussed thorough a simulation and with actual measurements with up-scaled test vehicles.

The laser micro-machining system for diamond anvil cell experiments and general precision machining applications at the High Pressure Collaborative Access Team

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hrubiak, Rostislav; Sinogeikin, Stanislav; Rod, Eric

We have designed and constructed a new system for micro-machining parts and sample assemblies used for diamond anvil cells and general user operations at the High Pressure Collaborative Access Team, sector 16 of the Advanced Photon Source. The new micro-machining system uses a pulsed laser of 400 ps pulse duration, ablating various materials without thermal melting, thus leaving a clean edge. With optics designed for a tight focus, the system can machine holes any size larger than 3 μm in diameter. Unlike a standard electrical discharge machining drill, the new laser system allows micro-machining of non-conductive materials such as: amorphousmore » boron and silicon carbide gaskets, diamond, oxides, and other materials including organic materials such as polyimide films (i.e., Kapton). An important feature of the new system is the use of gas-tight or gas-flow environmental chambers which allow the laser micro-machining to be done in a controlled (e.g., inert gas) atmosphere to prevent oxidation and other chemical reactions in air sensitive materials. The gas-tight workpiece enclosure is also useful for machining materials with known health risks (e.g., beryllium). Specialized control software with a graphical interface enables micro-machining of custom 2D and 3D shapes. The laser-machining system was designed in a Class 1 laser enclosure, i.e., it includes laser safety interlocks and computer controls and allows for routine operation. Though initially designed mainly for machining of the diamond anvil cell gaskets, the laser-machining system has since found many other micro-machining applications, several of which are presented here.« less

Alterations of neocortico-limbic association fibers and correlation with diet in prediabetes diagnosed by impaired fasting glucose.

PubMed

Hou, Yi-Cheng; Yang, Shwu-Huey; Wu, Yu-Te; Lai, Chien-Han

2016-06-01

To assess the existence of alterations in the micro-integrity of the fasciculus in prediabetic subjects. The issue of micro-integrity in white matter tracts has not been adequately addressed in prediabetes. Sixty-four prediabetic subjects and 54 controls were enrolled. All participants completed 24-hour diet records and 3-day diet records and received diffusion tensor imaging at 3T. The data for white matter micro-integrity were analyzed and compared between prediabetic subjects and controls with age and gender as covariates. In addition, voxel-wise regression between white matter micro-integrity, diet, and preprandial glucose levels were used to explore the relationship between white matter micro-integrity and diet or serum glucose levels. We found that prediabetic subjects had significant reductions in the micro-integrity of bilateral anterior thalamic radiation, left inferior longitudinal fasciculus, and left superior longitudinal fasciculus (corrected P < 0.05). In addition, total carbohydrate intake amount and preprandial serum glucose levels were negatively correlated with the micro-integrity in the left inferior longitudinal fasciculus and left anterior thalamic radiation (r: -0.47, corrected P < 0.05). Restrictive alterations in the white matter micro-integrity of the anterior thalamic radiation and inferior and superior longitudinal fasciculi might represent the initial "hot spots" for white matter tract alterations, which might play a role in the development of prediabetes. J. Magn. Reson. Imaging 2016;43:1500-1506. © 2016 Wiley Periodicals, Inc.

Large-area one-step assembly of three-dimensional porous metal micro/nanocages by ethanol-assisted femtosecond laser irradiation for enhanced antireflection and hydrophobicity.

PubMed

Li, Guoqiang; Li, Jiawen; Zhang, Chenchu; Hu, Yanlei; Li, Xiaohong; Chu, Jiaru; Huang, Wenhao; Wu, Dong

2015-01-14

The capability to realize 2D-3D controllable metallic micro/nanostructures is of key importance for various fields such as plasmonics, electronics, bioscience, and chemistry due to unique properties such as electromagnetic field enhancement, catalysis, photoemission, and conductivity. However, most of the present techniques are limited to low-dimension (1D-2D), small area, or single function. Here we report the assembly of self-organized three-dimensional (3D) porous metal micro/nanocages arrays on nickel surface by ethanol-assisted femtosecond laser irradiation. The underlying formation mechanism was investigated by a series of femtosecond laser irradiation under exposure time from 5 to 30 ms. We also demonstrate the ability to control the size of micro/nanocage arrays from 0.8 to 2 μm by different laser pulse energy. This method features rapidness (∼10 min), simplicity (one-step process), and ease of large-area (4 cm(2) or more) fabrication. The 3D cagelike micro/nanostructures exhibit not only improved antireflection from 80% to 7% but also enhanced hydrophobicity from 98.5° to 142° without surface modification. This simple technique for 3D large-area controllable metal microstructures will find great potential applications in optoelectronics, physics, and chemistry.

A Micro-delivery Approach for Studying Microvascular Responses to Localized Oxygen Delivery

PubMed Central

Ghonaim, Nour W.; Lau, Leo W. M.; Goldman, Daniel; Ellis, Christopher G.; Yang, Jun

2011-01-01

In vivo video microscopy has been used to study blood flow regulation as a function of varying oxygen concentration in microcirculatory networks. However, previous studies have measured the collective response of stimulating large areas of the microvascular network at the tissue surface. Objective We aim to limit the area being stimulated by controlling oxygen availability to highly localized regions of the microvascular bed within intact muscle. Design and Method Gas of varying O2 levels was delivered to specific locations on the surface of the Extensor Digitorum Longus muscle of rat through a set of micro-outlets (100 μm diameter) patterned in ultrathin glass using state-of-the-art microfabrication techniques. O2 levels were oscillated and digitized video sequences were processed for changes in capillary hemodynamics and erythrocyte O2 saturation. Results and Conclusions Oxygen saturations in capillaries positioned directly above the micro-outlets were closely associated with the controlled local O2 oscillations. Radial diffusion from the micro-outlet is limited to ~75 μm from the center as predicted by computational modelling and as measured in vivo. These results delineate a key step in the design of a novel micro-delivery device for controlled oxygen delivery to the microvasculature to understand fundamental mechanisms of microvascular regulation of O2 supply. PMID:21914035

Advanced MicroObserver UGS integration with and cueing of the BattleHawk squad level loitering munition and UAV

NASA Astrophysics Data System (ADS)

Steadman, Bob; Finklea, John; Kershaw, James; Loughman, Cathy; Shaffner, Patti; Frost, Dean; Deller, Sean

2014-06-01

Textron's Advanced MicroObserver(R) is a next generation remote unattended ground sensor system (UGS) for border security, infrastructure protection, and small combat unit security. The original MicroObserver(R) is a sophisticated seismic sensor system with multi-node fusion that supports target tracking. This system has been deployed in combat theaters. The system's seismic sensor nodes are uniquely able to be completely buried (including antennas) for optimal covertness. The advanced version adds a wireless day/night Electro-Optic Infrared (EOIR) system, cued by seismic tracking, with sophisticated target discrimination and automatic frame capture features. Also new is a field deployable Gateway configurable with a variety of radio systems and flexible networking, an important upgrade that enabled the research described herein. BattleHawkTM is a small tube launched Unmanned Air Vehicle (UAV) with a warhead. Using transmitted video from its EOIR subsystem an operator can search for and acquire a target day or night, select a target for attack, and execute terminal dive to destroy the target. It is designed as a lightweight squad level asset carried by an individual infantryman. Although BattleHawk has the best loiter time in its class, it's still relatively short compared to large UAVs. Also it's a one-shot asset in its munition configuration. Therefore Textron Defense Systems conducted research, funded internally, to determine if there was military utility in having the highly persistent MicroObserver(R) system cue BattleHawk's launch and vector it to beyond visual range targets for engagement. This paper describes that research; the system configuration implemented, and the results of field testing that was performed on a government range early in 2013. On the integrated system that was implemented, MicroObserver(R) seismic detections activated that system's camera which then automatically captured images of the target. The geo-referenced and time-tagged MicroObserver(R) target reports and images were then automatically forwarded to the BattleHawk Android-based controller. This allowed the operator to see the intruder (classified and geo-located) on the map based display, assess the intruder as likely hostile (via the image), and launch BattleHawk with the pre-loaded target coordinates. The operator was thus able to quickly acquire the intended target (without a search) and initiate target engagement immediately. System latencies were a major concern encountered during the research.

Using large volume samplers for the monitoring of particle bound micro pollutants in rivers

NASA Astrophysics Data System (ADS)

Kittlaus, Steffen; Fuchs, Stephan

2015-04-01

The requirements of the WFD as well as substance emission modelling at the river basin scale require stable monitoring data for micro pollutants. The monitoring concepts applied by the local authorities as well as by many scientists use single sampling techniques. Samples from water bodies are usually taken in volumes of about one litre and depending on predetermined time steps or through discharge thresholds. For predominantly particle bound micro pollutants the small sample size of about one litre results in a very small amount of suspended particles. To measure micro pollutant concentrations in these samples is demanding and results in a high uncertainty of the measured concentrations, if the concentration is above the detection limit in the first place. In many monitoring programs most of the measured values were below the detection limit. This results in a high uncertainty if river loads were calculated from these data sets. The authors propose a different approach to gain stable concentration values for particle bound micro pollutants from river monitoring: A mixed sample of about 1000 L was pumped in a tank with a dirty-water pump. The sampling usually is done discharge dependant by using a gauge signal as input for the control unit. After the discharge event is over or the tank is fully filled, the suspended solids settle in the tank for 2 days. After this time a clear separation of water and solids can be shown. A sample (1 L) from the water phase and the total mass of the settled solids (about 10 L) are taken to the laboratory for analysis. While the micro pollutants can't hardly be detected in the water phase, the signal from the sediment is high above the detection limit, thus certain and very stable. From the pollutant concentration in the solid phase and the total tank volume the initial pollutant concentration in the sample can be calculated. If the concentration in the water phase is detectable, it can be used to correct the total load. This relatively low cost approach (less costs for analysis because of small sample number) allows to quantify the pollutant load, to derive dissolved-solid partition coefficients and to quantify the pollutant load in different particle size classes.

The influence of flywheel micro vibration on space camera and vibration suppression

NASA Astrophysics Data System (ADS)

Li, Lin; Tan, Luyang; Kong, Lin; Wang, Dong; Yang, Hongbo

2018-02-01

Studied the impact of flywheel micro vibration on a high resolution optical satellite that space-borne integrated. By testing the flywheel micro vibration with six-component test bench, the flywheel disturbance data is acquired. The finite element model of the satellite was established and the unit force/torque were applied at the flywheel mounting position to obtain the micro vibration data of the camera. Integrated analysis of the data of the two parts showed that the influence of flywheel micro vibration on the camera is mainly concentrated around 60-80 Hz and 170-230 Hz, the largest angular displacement of the secondary mirror along the optical axis direction is 0.04″ and the maximum angular displacement vertical to optical axis is 0.032″. After the design and installation of vibration isolator, the maximum angular displacement of the secondary mirror is 0.011″, the decay rate of root mean square value of the angular displacement is more than 50% and the maximum is 96.78%. The whole satellite was suspended to simulate the boundary condition on orbit; the imaging experiment results show that the image motion caused by the flywheel micro vibrationis less than 0.1 pixel after installing the vibration isolator.

Discriminating between tectonism and climate signatures in palustrine deposits: Lessons from the Miocene of the Teruel Graben, NE Spain

NASA Astrophysics Data System (ADS)

Alonso-Zarza, Ana M.; Meléndez, Alfonso; Martín-García, Rebeca; Herrero, Mª Josefa; Martín-Pérez, Andrea

2012-07-01

The Upper Miocene (Vallesian-Turolian) Unit II of the Teruel Graben comprises at its top a 25 m-thick sequence of palustrine deposits. Deposition of the entire unit commenced some 9 to 7 Ma ago in a half-graben basin. Here, via a recent quarry, we examine in detail the lateral and vertical distribution of Unit II's palustrine facies and their features to determine the palaeogeography and main controls on deposit formation. Our findings suggest the deposits formed at a low-gradient lake margin with different energy levels. These energy levels controlled the type of primary deposit within the lake; wackestone to packstone sediments formed in low-energy conditions, whereas cross-bedded rudstones to floatstones formed under higher energy conditions, by erosion and redeposition of prior lacustrine deposits. Pedogenic and diagenetic modifications of the primary sediments took place during sedimentary discontinuities (SD) when the lacustrine sediments were subaerially exposed. These processes serve to explain the formation of eight different palustrine limestones: limestones with root traces, mottled limestones, brecciated limestones, flat pebble breccias, granular limestones, micro-karstified limestones with laminar calcretes, carbonate mounds and clayey limestones with laminar calcretes. Based on the features and thicknesses of the modified sediments five different morphological stages (I to V) of palustrine carbonates are defined. Stage I is characterized by incipient mottling and brecciation. Stage II shows mottling and strong brecciation that lead to the formation of intraclast breccias, in which the fragments are mostly "in situ". In Stage III, the primary fabric is totally changed; intraclasts have moved and may have lost their initial morphology. This Stage III may also be characterized by the formation of micro-karst. Stage IV is typified by the presence of coated grains and thin root mats. The chronological data available suggest that the formation of Stage III (lacustrine deposition + palustrine modification) would require about 40,000 yr. Facies and the SD record changes across short horizontal distances, and thus reflect the topography of prior sedimentation/modification events. Small (50 cm) highs with micro-karst have their SD counterparts in lower areas of the lake, in which the SD is indicated by desiccation and mottling. The topographic differences of the micro-karst were filled by intraclastic rudstones sourced by the adjacent carbonate flats. The example examined not only clearly sketches the morphology of ancient palustrine systems or wetlands, it also provides evidence that recycling of previous carbonate deposits played an important role as a sediment source, apart from biogenic or physical-chemical production processes. Our geochemical data indicate LMC (Low Magnesian Calcite) as the main component and Fe contents lower than 1%, except for the mottled areas that are richer in FeO. Stable isotope compositions provide δ18O values close to - 6.5‰ VPDB, and more varied δ13C (- 3.39 to - 6.97‰ PDB). Oxygen and carbon values reveal no covariation and clear trends are lacking. The homogeneity of δ18O values reflects the intense effects of meteoric waters. The deposition of these palustrine limestones took place under suitable semi-arid to sub-humid climates. Climate could also have a role in determining subaerial exposure periods. However, its imprint is not easy to detect neither in the geochemical signals nor in the vertical arrangement of the facies. This could be attributed to climate changes probably occurring over shorter periods than those that can be recorded in this type of sediment, such as the astronomical precession cycles, and suggests the unsuitability of palustrine carbonates for detailed palaeoclimate analyses. Tectonism controlled the location of the main lacustrine depocentre close to the basin's main fault. The activity of this normal fault during the sedimentation of Unit II determined long- and short-term sedimentary sequences. Such sequences are the response to small-scale subsidence pulses followed by the infill of the created accommodation space by shallow lacustrine deposits, which underwent early pedogenic and diagenetic processes after subaerial exposure.

International testing of a Mars rover prototype

NASA Astrophysics Data System (ADS)

Kemurjian, Alexsandr Leonovich; Linkin, V.; Friedman, L.

1993-03-01

Tests on a prototype engineering model of the Russian Mars 96 Rover were conducted by an international team in and near Death Valley in the United States in late May, 1992. These tests were part of a comprehensive design and testing program initiated by the three Russian groups responsible for the rover development. The specific objectives of the May tests were: (1) evaluate rover performance over different Mars-like terrains; (2) evaluate state-of-the-art teleoperation and autonomy development for Mars rover command, control and navigation; and (3) organize an international team to contribute expertise and capability on the rover development for the flight project. The range and performance that can be planned for the Mars mission is dependent on the degree of autonomy that will be possible to implement on the mission. Current plans are for limited autonomy, with Earth-based teleoperation for the nominal navigation system. Several types of television systems are being investigated for inclusion in the navigation system including panoramic camera, stereo, and framing cameras. The tests used each of these in teleoperation experiments. Experiments were included to consider use of such TV data in autonomy algorithms. Image processing and some aspects of closed-loop control software were also tested. A micro-rover was tested to help consider the value of such a device as a payload supplement to the main rover. The concept is for the micro-rover to serve like a mobile hand, with its own sensors including a television camera.

rs4919510 in hsa-mir-608 Is Associated with Outcome but Not Risk of Colorectal Cancer

PubMed Central

Valeri, Nicola; Robinson, Dillon; Paone, Alessio; Bowman, Elise D.; Robles, Ana I.; Croce, Carlo; Harris, Curtis C.

2012-01-01

Background Colorectal cancer is the third most incident cancer and cause of cancer-related death in the United States. MicroRNAs, a class of small non-coding RNAs, have been implicated in the pathogenesis and prognosis of colorectal cancer, although few studies have examined the relationship between germline mutation in the microRNAs with risk and prognosis. We therefore investigated the association between a SNP in hsa-mir-608, which lies within the 10q24 locus, and colorectal cancer. Methods and Results A cohort consisting of 245 cases and 446 controls was genotyped for rs4919510. The frequency of the GG genotype was significantly higher in African Americans (15%) compared to Caucasians (3%) controls. There was no significant association between rs4919510 and colorectal cancer risk (African American: ORGG vs. CC 0.89 [95% CI, 0.41–1.80]) (Caucasian: ORGG vs. CC 1.76, ([95% CI, 0.48–6.39]). However, we did observe an association with survival. The GG genotype was associated with an increased risk of death in Caucasians (HRGG vs. CC 3.54 ([95% CI, 1.38–9.12]) and with a reduced risk of death in African Americans (HRGG vs. CC 0.36 ([95% CI 0.12–1.07). Conclusions These results suggest that rs4910510 may be associated with colorectal cancer survival in a manner that is dependent on race. PMID:22606253

The governance of innovation diffusion - a socio-technical analysis of energy policy

NASA Astrophysics Data System (ADS)

Nolden, C.

2012-10-01

This paper describes a dynamic price mechanism to coordinate eletric power generation from micro Combined Heat and Power (micro-CHP) systems in a network of households. It is assumed that the households are prosumers, i.e. both producers and consumers of electricity. The control is done on household level in a completely distributed manner. Avoiding a centralized controller both eases computation complexity and preserves communication structure in the network. Local information is used to decide to turn on or off the micro-CHP, but through price signals between the prosumers the network as a whole operates in a cooperative way.

Design of micro-second pulsed laser mode for ophthalmological CW self-raman laser

NASA Astrophysics Data System (ADS)

Mota, Alessandro D.; Rossi, Giuliano; Ortega, Tiago A.; Costal, Glauco Z.; Fontes, Yuri C.; Yasuoka, Fatima M. M.; Stefani, Mario A.; de Castro N., Jarbas C.; Paiva, Maria S. V.

2011-02-01

This work presents the mechanisms adopted for the design of micro-second pulsed laser mode for a CW Self-Raman laser cavity in 586nm and 4W output power. The new technique for retina disease treatment discharges laser pulses on the retina tissue, in laser sequences of 200 μs pulse duration at each 2ms. This operation mode requires the laser to discharge fast electric pulses, making the system control velocity of the electronic system cavity vital. The control procedures to keep the laser output power stable and the laser head behavior in micro-second pulse mode are presented.

Astronomical Research with the MicroObservatory Net

NASA Astrophysics Data System (ADS)

Brecher, K.; Sadler, P.; Gould, R.; Leiker, S.; Antonucci, P.; Deutsch, F.

1997-05-01

We have developed a fully integrated automated astronomical telescope system which combines the imaging power of a cooled CCD, with a self-contained and weatherized 15 cm reflecting optical telescope and mount. The MicroObservatory Net consists of five of these telescopes. They are currently being deployed around the world at widely distributed longitudes. Remote access to the MicroObservatories over the Internet has now been implemented. Software for computer control, pointing, focusing, filter selection as well as pattern recognition have all been developed as part of the project. The telescopes can be controlled in real time or in delay mode, from a Macintosh, PC or other computer using Web-based software. The Internet address of the telescopes is http://cfa- www.harvard.edu/cfa/sed/MicroObservatory/MicroObservatory.html. In the real-time mode, individuals have access to all of the telescope control functions without the need for an `on-site' operator. Users can sign up for a specific period of ti me. In the batch mode, users can submit requests for delayed telescope observations. After a MicroObservatory completes a job, the user is automatically notified by e-mail that the image is available for viewing and downloading from the Web site. The telescopes were designed for classroom instruction, as well as for use by students and amateur astronomers for original scientific research projects. We are currently examining a variety of technical and educational questions about the use of the telescopes including: (1) What are the best approaches to scheduling real-time versus batch mode observations? (2) What criteria should be used for allocating telescope time? (3) With deployment of more than one telescope, is it advantageous for each telescope to be used for just one type of observation, i.e., some for photometric use, others for imaging? And (4) What are the most valuable applications of the MicroObservatories in astronomical research? Support for the MicroObservatory Net has been provided by the NSF, Apple Computer, Inc. and Kodak, Inc.

Nonlinear piezoelectric effects—towards physics-based computational modelling of micro-cracking, fatigue, and switching

NASA Astrophysics Data System (ADS)

Menzel, A.; Utzinger, J.; Arockiarajan, A.

2008-07-01

Piezoelectric ceramics—as one widely commercialised group of smart materials—exhibit a great potential for various engineering applications. Their high-frequency capabilities are in particular attractive for actuator and sensor devices, which nowadays are present in daily-life-technologies such as cellular phones, fuel injection systems, and so forth. At high loading levels however, severely nonlinear behaviour of these materials is observed which, from the control point of view, must be further investigated in order to be able to precisely account for such effects within the design of intelligent systems. The reasons for these nonlinearities are manifold and, even investigated within the last decades, not fully understood. Nevertheless, two important sources for these observations are so-called micro-cracking, together with fatigue phenomena, as well as switching or rather phase transformations. Accordingly, the main goal of this contribution is to study these effects by means of developing related constitutive models that can be embedded into iterative algorithmic schemes such as the finite element method. One the one hand, the grain-structure of a piezoceramic specimen will be modelled via the direct incorporation of the grain-boundaries as so-called interface elements. The underlying cohesive-like constitutive law of this layer includes both degrees of freedom of the surrounding bulk material—or rather the jumps in these fields—namely displacements and the electric potential. Based on the resulting traction-separation-type relations, micro-cracking is directly accounted for on this microlevel. Moreover, the constitutive law of the interfacial layer is supplemented by additional variables that enable the formulation of fatigue under cyclic loading conditions. On the other hand, phase transformations—modelled in terms of an energy-based switching criterion—are discussed and embedded into an iterative finite element context. Symmetry relations of the underlying unit cells are directly included so that the switching model accounts for the micro-mechanical properties of the piezoelectric materials of interest. At this stage, representative numerical simulations of polycrystalline specimens are based on straightforward averaging-techniques, while the combination of the developed micro-cracking model (grain boundaries) with the proposed switching model (bulk) constitutes future research.

Gas Turbine Heavy Hybrid Powertrain Variants. Opportunities and Potential for Systems Optimization

DOE Office of Scientific and Technical Information (OSTI.GOV)

Smith, David; Chambon, Paul H.

2015-07-01

Widespread use of alternative hybrid powertrains is currently inevitable, and many opportunities for substantial progress remain. Hybrid electric vehicles (HEVs) have attracted considerable attention due to their potential to reduce petroleum consumption and greenhouse gas emissions in the transportation sector. This capability is mainly attributed to (a) the potential for downsizing the engine, (b) the potential for recovering energy during braking and thus recharging the energy storage unit, and (c) the ability to minimize the operation of the engine outside of its most efficient brake specific fuel consumption (BSFC) regime. Hybridization of the Class 8, heavy-duty (HD) powertrain is inherentlymore » challenging due to the expected long-haul driving requirements and limited opportunities for regenerative braking. The objective of this project is to develop control strategies aiming at optimizing the operation of a Class 8 HEV that features a micro-turbine as the heat engine. The micro-turbine application shows promise in fuel efficiency, even when compared to current diesel engines, and can meet regulated exhaust emissions levels with no exhaust after-treatment system. Both parallel and series HEV variants will be examined to understand the merits of each approach of the micro-turbine to MD advanced powertrain applications. These powertrain configurations enable new paradigms in operational efficiency, particularly in the Class 8 truck fleet. The successful development of these HEV variants will require a thorough technical understanding of the complex interactions between various energy sources and energy consumption components, for various operating modes. PACCAR will be integrating the first generation of their series HEV powertrain with a Brayton Energy micro-turbine into a Class 8 HD truck tractor that has both regional haul and local pick-up and delivery (P&D) components to its drive cycle. The vehicle will be deployed into fleet operation for a demonstration period of six (6) months to assess real world operating benefits of the advanced powertrain. A parallel variant of the micro-turbine powertrain will be built and sent to the ORNL Vehicle Systems Integration Laboratory.« less

Two-Phase Flow in High-Heat-Flux Micro-Channel Heat Sink for Refrigeration Cooling Applications. Part 1: Micro-Channel Heat Sink for Direct Refrigeration Cooling

DTIC Science & Technology

2008-09-01

Mudawar Jaeseon Lee Myungki Sung Boiling and Two-Phase Flow Laboratory School of Mechanical Engineering Purdue University West Lafayette, Indiana...NA 6. AUTHOR(S) 5d. PROJECT NUMBER Mudawar , Issam NA Lee, Jaeseon Sung, Myung Ki 5e. TASK NUMBER NA 5f. WORK UNIT NUMBER NA 7. PERFORMING...NAME OF RESPONSIBLE PERSON a. REPORT b. ABSTRACT c. THIS PAGE ABSTRACT OF Mudawar , Issam PAGES U UU 19b. TELEPHONE NUMBER (Include area code)U U 465 765

A software upgrade method for micro-electronics medical implants.

PubMed

Cao, Yang; Hao, Hongwei; Xue, Lin; Li, Luming; Ma, Bozhi

2006-01-01

A software upgrade method for micro-electronics medical implants is designed to enhance the devices' function or renew the software if there are some bugs found, the software updating or some memory units disabled. The implants needn't be replaced by operations if the faults can be corrected through reprogramming, which reduces the patients' pain and improves the safety effectively. This paper introduces the software upgrade method using in-application programming (IAP) and emphasizes how to insure the system, especially the implanted part's reliability and stability while upgrading.

Lateral-deflection-controlled friction force microscopy

NASA Astrophysics Data System (ADS)

Fukuzawa, Kenji; Hamaoka, Satoshi; Shikida, Mitsuhiro; Itoh, Shintaro; Zhang, Hedong

2014-08-01

Lateral-deflection-controlled dual-axis friction force microscopy (FFM) is presented. In this method, an electrostatic force generated with a probe-incorporated micro-actuator compensates for friction force in real time during probe scanning using feedback control. This equivalently large rigidity can eliminate apparent boundary width and lateral snap-in, which are caused by lateral probe deflection. The method can evolve FFM as a method for quantifying local frictional properties on the micro/nanometer-scale by overcoming essential problems to dual-axis FFM.

Theory for controlling individual self-propelled micro-swimmers by photon nudging II: confinement.

PubMed

Selmke, Markus; Khadka, Utsab; Bregulla, Andreas P; Cichos, Frank; Yang, Haw

2018-04-18

Photon nudging allows the manipulation and confinement of individual self-propelled micro-swimmers in 2D and 3D environments using feedback controls. Presented in this second part of a two-part contribution are theoretical models that afford the characterization for the positioning distribution associated with active localization. A derivation for the optimal nudging speed and acceptance angle is given for minimal placement uncertainty. The analytical solutions allow for a discussion on the physical underpinning that underlies controllability and optimality.

Micro-Inspector Spacecraft Testbed: Breadboard Subsystem Demonstrations

NASA Astrophysics Data System (ADS)

Mueller, Juergen; Goldberg, Hannah; Alkalai, Leon

2007-01-01

Micro-inspector is a 5-kg inspection platform designed to operate autonomously following operator up-linked command sequences around a host spacecraft to perform safety inspections, anomaly inspections, or imaging of large in-space assemblies as envisioned for future NASA exploration missions. Similarly, such an inspection platform may be adapted to military space missions. Micro-inspector relies on solar power and using celestial sensors for navigation, giving the system large flexibility in the missions and applications it may serve, including those beyond Earth orbit. Micro-Inspector, through its small size and low weight, poses minimal design impacts to the host. Its small size and weight also affords micro-inspector to be disposable, allowing multiple inspectors to be used by a single host for different inspection routines or as emergency back-up. Its low-pressure butane propulsion system combines safety and compactness through liquid propellant storage with an adequate performance of up to 30 m/s for inspection maneuvers around the host. Micro-inspector, since power limited through a body mounted solar array, thus avoiding the complexities of deployable structures, relies on many advanced, ultra-low power micro-technologies, such as a novel microvalve by VACCO Industries in its propulsion system, electrochromic surface modulating heat transfer from the spacecraft using no moving parts, low power dual processor and FPGA-based reconfigurable and SEU mitigating avionics, a low power RF telecom link based on the Mars Micro Transceiver, and micro attitude control sensors, such as commercial micro IMUs and a JPL developed micro sun sensor. Host safety is a key concern, and multiple safety features are employed by micro-inspector to prevent any accidental impact onto the host. Among these is an active, laser-based range-finding collision avoidance system, which constantly monitors the distance to the host and via the micro-inspector's control system maintains a safe distance. Micro-Inspector design, through funding from the NASA Explorations Systems Mission Directorate, has significantly advanced over the past year and is currently at PDR level and beyond. Special emphasis was placed on retiring risk in various subsystem areas through the use of advanced technologies. To this end, a micro-inspector test bed was set up to critically assess the readiness of component technologies and subsystems. Breadboard subsystem demonstrations and system integration were performed to place future design efforts on a solid basis.

Modelling and Dosimetry for Alpha-Particle Therapy

PubMed Central

Sgouros, George; Hobbs, Robert F.; Song, Hong

2015-01-01

As a consequence of the high potency and short range of alpha-particles, radiopharmaceutical therapy with alpha-particle emitting radionuclides is a promising treatment approach that is under active pre-clinical and clinical investigation. To understand and predict the biological effects of alpha-particle radiopharmaceuticals, dosimetry is required at the micro or multi-cellular scale level. At such a scale, highly non-uniform irradiation of the target volume may be expected and the utility of a single absorbed dose value to predict biological effects comes into question. It is not currently possible to measure the pharmacokinetic input required for micro scale dosimetry in humans. Accordingly, pre-clinical studies are required to provide the pharmacokinetic data for dosimetry calculations. The translation of animal data to the human requires a pharmacokinetic model that links macro- and micro-scale pharmacokinetics thereby enabling the extrapolation of micro-scale kinetics from macroscopic measurements. These considerations along with a discussion of the appropriate physical quantity and related units for alpha-particle radiopharmaceutical therapy are examined in this review. PMID:22201712

Hybrid micro-scale photovoltaics for enhanced energy conversion across all irradiation conditions

NASA Astrophysics Data System (ADS)

Agrawal, Gautam

A novel hybrid photovoltaics (HPV) architecture is presented that integrates high-performance micro-optics-based concentrator photovoltaics (CPV) array technology with a 1-sun photovoltaic (PV) cell within a low-profile panel structure. The approach simultaneously captures the direct solar radiation components with arrayed high-efficiency CPV cells and the diffuse solar components with an underlying wide-area PV cell. Performance analyses predict that the hybrid approach will significantly enhance the average energy produced per unit area for the full range of diffuse/direct radiation patterns across the USA. Furthermore, cost analyses indicate that the hybrid concept may be financially attractive for a wide range of locations. Indoor and outdoor experimental evaluation of a micro-optical system designed for use in a hybrid architecture verified that a large proportion of the direct radiation component was concentrated onto emulated micro-cell regions while most of the diffuse radiation and the remaining direct radiation was collected in the 1-sun cell area.

Photon-trapping micro/nanostructures for high linearity in ultra-fast photodiodes

NASA Astrophysics Data System (ADS)

Cansizoglu, Hilal; Gao, Yang; Perez, Cesar Bartolo; Ghandiparsi, Soroush; Ponizovskaya Devine, Ekaterina; Cansizoglu, Mehmet F.; Yamada, Toshishige; Elrefaie, Aly F.; Wang, Shih-Yuan; Islam, M. Saif

2017-08-01

Photodetectors (PDs) in datacom and computer networks where the link length is up to 300 m, need to handle higher than typical input power used in other communication links. Also, to reduce power consumption due to equalization at high speed (>25Gb/s), the datacom links will use PAM-4 signaling instead of NRZ with stringent receiver linearity requirements. Si PDs with photon-trapping micro/nanostructures are shown to have high linearity in output current verses input optical power. Though there is less silicon material due to the holes, the micro-/nanostructured holes collectively reradiate the light to an in-plane direction of the PD surface and can avoid current crowding in the PD. Consequently, the photocurrent per unit volume remains at a low level contributing to high linearity in the photocurrent. We present the effect of design and lattice patterns of micro/nanostructures on the linearity of ultra-fast silicon PDs designed for high speed multi gigabit data networks.

Electrophoretic separation of cells and particles from rat pituitary and rat spleen

NASA Technical Reports Server (NTRS)

Hymer, Wesley C.

1993-01-01

There are 3 parts to the IML-2 TX-101 experiment. Part 1 is a pituitary cell culture experiment. Part 2 is a pituitary cell separation experiment using the Japanese free flow electrophoresis unit (FFEU). Part 3 is a pituitary secretory granule separation experiment using the FFEU. The objectives of this three part experiment are: (1) to determine the kinetics of production of biologically active growth hormone (GH) and prolactin (PRL) in rat pituitary GH and PRL cells in microgravity (micro-g); (2) to investigate three mechanisms by which a micro-g-induced lesion in hormone production may occur; and (3) to determine the quality of separations of pituitary cells and organelles by continuous flow electrophoresis (CFE) in micro-g under conditions where buoyancy-induced convection is eliminated.

Groove micro-structure optical fiber refractive index sensor with nanoscale gold film based on surface plasmon resonance

NASA Astrophysics Data System (ADS)

Zhang, Zhen; Li, Shuguang; Liu, Qiang; Feng, Xinxing; Zhang, Shuhuan; Wang, Yujun; Wu, Junjun

2018-07-01

A groove micro-structure optical fiber refractive index sensor with nanoscale gold film based on surface plasmon resonance (SPR) is proposed and analyzed by the finite element method (FEM). Numerical results show that the average sensitivity is 15,933 nm/refractive index unit (RIU) with the refractive index of analyte ranging from 1.40 to 1.43 and the maximum sensitivity is 28,600 nm/RIU and the resolution of the sensor is 3.50 × 10-8 RIU. The groove micro-structure optical fiber refractive index sensor do some changes on the D-shaped fiber sensor, compared with conventional D-shaped fiber sensor, it has a higher sensitivity and it is easier to produce than the traditional SPR sensor.

A Micro-Computed Tomography Technique to Study the Quality of Fibre Optics Embedded in Composite Materials

PubMed Central

Chiesura, Gabriele; Luyckx, Geert; Voet, Eli; Lammens, Nicolas; Van Paepegem, Wim; Degrieck, Joris; Dierick, Manuel; Van Hoorebeke, Luc; Vanderniepen, Pieter; Sulejmani, Sanne; Sonnenfeld, Camille; Geernaert, Thomas; Berghmans, Francis

2015-01-01

Quality of embedment of optical fibre sensors in carbon fibre-reinforced polymers plays an important role in the resultant properties of the composite, as well as for the correct monitoring of the structure. Therefore, availability of a tool able to check the optical fibre sensor-composite interaction becomes essential. High-resolution 3D X-ray Micro-Computed Tomography, or Micro-CT, is a relatively new non-destructive inspection technique which enables investigations of the internal structure of a sample without actually compromising its integrity. In this work the feasibility of inspecting the position, the orientation and, more generally, the quality of the embedment of an optical fibre sensor in a carbon fibre reinforced laminate at unit cell level have been proven. PMID:25961383

Microgravity alters protein phosphorylation changes during initiation of sea urchin sperm motility

NASA Technical Reports Server (NTRS)

Tash, J. S.; Bracho, G. E.

1999-01-01

European Space Agency (ESA) studies demonstrated that bull sperm swim with higher velocity in microgravity (microG) than at 1 G. Coupling between protein phosphorylation and sperm motility during activation in microG and at 1 G was examined in the ESA Biorack on two space shuttle missions. Immotile sperm were activated to swim (86-90% motility) at launch +20 h by dilution into artificial seawater (ASW). Parallel ground controls were performed 2 h after the flight experiment. Activation after 0, 30, and 60 s was terminated with electrophoresis sample buffer and samples analyzed for phosphoamino acids by Western blotting. Phosphorylation of a 130-kDa phosphothreonine-containing protein (FP130) occurred three to four times faster in microG than at 1 G. A 32-kDa phosphoserine-containing protein was significantly stimulated at 30 s but returned to 1 G control levels at 60 s. The rate of FP130 phosphorylation in microG was attenuated by D2O, suggesting that changes in water properties participate in altering signal transduction. Changes in FP130 phosphorylation triggered by the egg peptide speract were delayed in microG. These results demonstrate that previously observed effects of microG on sperm motility are coupled to changes in phosphorylation of specific flagellar proteins and that early events of sperm activation and fertilization are altered in microG.

Automated micromanipulation desktop station based on mobile piezoelectric microrobots

NASA Astrophysics Data System (ADS)

Fatikow, Sergej

1996-12-01

One of the main problems of present-day research on microsystem technology (MST) is to assemble a whole micro- system from different microcomponents. This paper presents a new concept of an automated micromanipulation desktop- station including piezoelectrically driven microrobots placed on a high-precise x-y-stage of a light microscope, a CCD-camera as a local sensor subsystem, a laser sensor unit as a global sensor subsystem, a parallel computer system with C167 microcontrollers, and a Pentium PC equipped additionally with an optical grabber. The microrobots can perform high-precise manipulations (with an accuracy of up to 10 nm) and a nondestructive transport (at a speed of about 3 cm/sec) of very small objects under the microscope. To control the desktop-station automatically, an advanced control system that includes a task planning level and a real-time execution level is being developed. The main function of the task planning sub-system is to interpret the implicit action plan and to generate a sequence of explicit operations which are sent to the execution level of the control system. The main functions of the execution control level are the object recognition, image processing and feedback position control of the microrobot and the microscope stage.

Externally resonated linear microvibromotor for microassembly

NASA Astrophysics Data System (ADS)

Saitou, Kazuhiro; Wou, Soungjin J.

1998-10-01

A new design of a linear micro vibromotor for on-substrate fine positioning of micro-scale components is presented where a micro linear slider is actuated by vibratory impacts exerted by micro cantilever impacters. These micro cantilever impacters are selectively resonated by shaking the entire substrate with a piezoelectric vibrator, requiring no need for built-in driving mechanisms such as electrostatic comb actuators as reported previously. This selective resonance of the micro cantilever impacters via an external vibration energy field provides with a very simple means of controlling forward and backward motion of the micro linear slider, facilitating assembly and disassembly of a micro component on a substrate. The double-V beam suspension design is employed in the micro cantilever impacters for larger displacement in the lateral direction while achieving higher stiffness in the transversal direction. An analytical model of the device is derived in order to obtain, through the Simulated Annealing algorithm, an optimal design which maximizes translation speed of the linear slider at desired external input frequencies. Prototypes of the externally-resonated linear micro vibromotor are fabricated using the three-layer polysilicon surface micro machining process provided by the MCNC MUMPS service.

Time Evolution of the Density Field of a Micro-Explosion Using Background Oriented Schlieren

NASA Astrophysics Data System (ADS)

Suriyanarayanan, P.; Venkatakrishnan, L.; Jagadeesh, G.

In recent years micro-explosions have found interesting trans-disciplinary applications in the areas of food preservation,wood science, drug delivery, gene therapy and bio-medical applications [1, 2]. Generating controlled micro-explosions in a laboratory environment in a reliable manner is essential; to study and understand some of the near field flow dynamics associated with blast waves.

Swiss roll nanomembranes with controlled proton diffusion as redox micro-supercapacitors.

PubMed

Ji, Hengxing; Mei, Yongfeng; Schmidt, Oliver G

2010-06-14

We demonstrate a redox Swiss roll micro-supercapacitor by rolling up a multilayered nanomembrane with an electrochemical active layer at either the outer or inner surface for different proton diffusion behaviors. The Swiss roll micro-supercapacitor could achieve high performance (e.g. capacity and life time) in a microscale power source and is helpful for studying charge transfer at the electrolyte/electrode interface.

Implementation of RCCL, a robot control C library on a microVAX II

NASA Technical Reports Server (NTRS)

Lee, Jin S.; Hayati, Samad; Hayward, Vincent; Lloyd, John E.

1987-01-01

The robot control C library (RCCL), a high-level robot programing system which enables a progammer to employ a set of system calls to specify robot manipulator tasks, is discussed. The general structure of RCCL is described, and the implementation of RCCL on a microVAX II is examined. Proposed extensions and improvements of RCCL relevant to NASA's telerobotic system are addressed.

Development of acousto-optic spatial light modulator unit for effective control of light beam intensity and diffraction angle in 3D holographic display applications

NASA Astrophysics Data System (ADS)

Kondalkar, Vijay V.; Ryu, Geonhee; Lee, Yongbeom; Lee, Keekeun

2018-07-01

An acousto-optic (AO) based holographic display unit was developed using surface acoustic wave (SAW) with different wavelength to modulate the diffraction angles, intensities, and phases of light. The new configurations were employed to control two beams simultaneously by using a single chirp inter-digital transducer (IDT), and a micro-lens array was integrated at the end of the waveguide layer to focus the diffracted light on to the screen. Two incident light beams were simultaneously modulated by using different refractive grating periods generated from chirp IDT. A diffraction angle of about 5° was obtained by using a SAW with a frequency of 430 MHz. The increase in the SAW input power enhances the diffraction efficiency of the light beam at the exit. The obtained maximum diffraction efficiency is ~70% at a frequency of 430 MHz. The sloped shape of the waveguide entrance and a tall rounded Ni poles help in coupling the incident light to the waveguide layer. The diffracted beam was collected through the lens, which increased the intensity of light in the viewing plane. COMSOL multi-physics and coupling of mode (COM) modeling were performed to predict the device performance and compared with the experimental results.

Micro-column plasma emission liquid chromatograph

DOEpatents

Gay, Don D.

1984-01-01

In a direct current plasma emission spectrometer for use in combination with a micro-column liquid chromatograph, an improved plasma source unit. The plasma source unit includes a quartz capillary tube having an inlet means, outlet off gas means and a pair of spaced electrodes defining a plasma region in the tube. The inlet means is connected to and adapted to receive eluant of the liquid chromatograph along with a stream of plasma-forming gas. There is an opening through the wall of the capillary tube penetrating into the plasma region. A soft glass capillary light pipe is disposed at the opening, is connected to the spectrometer, and is adapted to transmit light passing from the plasma region to the spectrometer. There is also a source of electromotive force connected to the electrodes sufficient to initiate and sustain a plasma in the plasma region of the tube.

FPGA Control System for the Automated Test of MicroShutters

NASA Technical Reports Server (NTRS)

Lyness, Eric; Rapchun, David A.; Moseley, S. Harvey

2008-01-01

The James Webb Space Telescope, scheduled to replace the Hubble in 2013, must simultaneously observe hundreds of faint galaxies. This requirement has led to the development of a programmable transmission mask which can be adapted to admit light from an arbitrary pattern of galaxies into its spectrograph. This programmable mask will contain a large array of micro-electromechanical (MEMs) devices called MicroShutters. These microscopic shutters physically open and close like the shutter on a camera, except each shutter is microscopic in size and an array 365 by 171 is used to select the objects under spectroscopic observation at a given time, and to block the unwanted background light from other areas. NASA developed and is currently refining the exceptionally difficult process of manufacturing these shutters. This paper describes how the authors used LabVIEW FPGA and a reconfigurable I/O board to control the shutters in a test chamber and how the flexibility of the system allows us to continue to modify the control algorithms as NASA optimizes the performance of the MicroShutter arrays.

Rapid Communication: MiR-92a as a housekeeping gene for analysis of bovine mastitis-related microRNA in milk.

PubMed

Lai, Y C; Fujikawa, T; Ando, T; Kitahara, G; Koiwa, M; Kubota, C; Miura, N

2017-06-01

Our aim was to identify a suitable microRNA housekeeping gene for real-time PCR analysis of bovine mastitis-related microRNA in milk. We identified , , and as housekeeping gene candidates on the basis of previous Solexa sequencing results. Threshold cycle (CT) values for , , and did not differ between milk from control cows and milk from mastitis-affected cows. NormFinder software identified as the most stable single housekeeping gene. We evaluated the suitability of the housekeeping gene candidates by using them to assess expression levels of the inflammation-related gene . Regardless of the housekeeping gene candidates used for normalization, relative expression levels of were significantly higher in mastitis-affected samples than in control samples. However, of all the housekeeping genes and gene combinations investigated, normalization with alone generated the difference in relative expression between mastitis-affected and control samples with the highest significance. These results suggest that is suitable for use as a housekeeping gene for analysis of bovine mastitis-related microRNA in milk.

Implementation of a sliding-mode-based position sensorless drive for high-speed micro permanent-magnet synchronous motors.

PubMed

Chi, Wen-Chun; Cheng, Ming-Yang

2014-03-01

Due to issues such as limited space, it is difficult if it is not impossible to employ a position sensor in the drive control of high-speed micro PMSMs. In order to alleviate this problem, this paper analyzes and implements a simple and robust position sensorless field-oriented control method of high-speed micro PMSMs based on the sliding-mode observer. In particular, the angular position and velocity of the rotor of the high-speed micro PMSM are estimated using the sliding-mode observer. This observer is able to accurately estimate rotor position in the low speed region and guarantee fast convergence of the observer in the high speed region. The proposed position sensorless control method is suitable for electric dental handpiece motor drives where a wide speed range operation is essential. The proposed sensorless FOC method is implemented using a cost-effective 16-bit microcontroller and tested in a prototype electric dental handpiece motor. Several experiments are performed to verify the effectiveness of the proposed method. © 2013 ISA. Published by Elsevier Ltd. All rights reserved.

Surface micro- and nano-texturing of stainless steel by femtosecond laser for the control of cell migration.

PubMed

Martínez-Calderon, M; Manso-Silván, M; Rodríguez, A; Gómez-Aranzadi, M; García-Ruiz, J P; Olaizola, S M; Martín-Palma, R J

2016-11-02

The precise control over the interaction between cells and the surface of materials plays a crucial role in optimizing the integration of implanted biomaterials. In this regard, material surface with controlled topographic features at the micro- and nano-scales has been proved to affect the overall cell behavior and therefore the final osseointegration of implants. Within this context, femtosecond (fs) laser micro/nano machining technology was used in this work to modify the surface structure of stainless steel aiming at controlling cell adhesion and migration. The experimental results show that cells tend to attach and preferentially align to the laser-induced nanopatterns oriented in a specific direction. Accordingly, the laser-based fabrication method here described constitutes a simple, clean, and scalable technique which allows a precise control of the surface nano-patterning process and, subsequently, enables the control of cell adhesion, migration, and polarization. Moreover, since our surface-patterning approach does not involve any chemical treatments and is performed in a single step process, it could in principle be applied to most metallic materials.

Surface micro- and nano-texturing of stainless steel by femtosecond laser for the control of cell migration

PubMed Central

Martínez-Calderon, M.; Manso-Silván, M.; Rodríguez, A.; Gómez-Aranzadi, M.; García-Ruiz, J. P.; Olaizola, S. M.; Martín-Palma, R. J.

2016-01-01

The precise control over the interaction between cells and the surface of materials plays a crucial role in optimizing the integration of implanted biomaterials. In this regard, material surface with controlled topographic features at the micro- and nano-scales has been proved to affect the overall cell behavior and therefore the final osseointegration of implants. Within this context, femtosecond (fs) laser micro/nano machining technology was used in this work to modify the surface structure of stainless steel aiming at controlling cell adhesion and migration. The experimental results show that cells tend to attach and preferentially align to the laser-induced nanopatterns oriented in a specific direction. Accordingly, the laser-based fabrication method here described constitutes a simple, clean, and scalable technique which allows a precise control of the surface nano-patterning process and, subsequently, enables the control of cell adhesion, migration, and polarization. Moreover, since our surface-patterning approach does not involve any chemical treatments and is performed in a single step process, it could in principle be applied to most metallic materials. PMID:27805063

Capabilities Eroded and Childcare Dilemmas: A Phenomenological Study of Low-Income Single Student Mothers in Higher Education in the United States and Germany

ERIC Educational Resources Information Center

Robinson, Katja

2016-01-01

The purpose of this phenomenological study was to explore the lifeworlds of low-income, single student mothers in two contrasting welfare regimes, the United States and Germany, to understand how their individual experiences as students and as mothers at the micro-level were shaped by policies regarding childcare and financial support at the…

Friend or Foe: MicroRNAs in the p53 network.

PubMed

Luo, Zhenghua; Cui, Ri; Tili, Esmerina; Croce, Carlo

2018-04-10

The critical tumor suppressor gene TP53 is either lost or mutated in more than half of human cancers. As an important transcriptional regulator, p53 modulates the expression of many microRNAs. While wild-type p53 uses microRNAs to suppress cancer development, microRNAs that are activated by gain-of-function mutant p53 confer oncogenic properties. On the other hand, the expression of p53 is tightly controlled by a fine-tune machinery including microRNAs. MicroRNAs can target the TP53 gene directly or other factors in the p53 network so that expression and function of either the wild-type or the mutant forms of p53 is downregulated. Therefore, depending on the wild-type or mutant p53 context, microRNAs contribute substantially to suppress or exacerbate tumor development. Copyright © 2018. Published by Elsevier B.V.

A micro-vibration generated method for testing the imaging quality on ground of space remote sensing

NASA Astrophysics Data System (ADS)

Gu, Yingying; Wang, Li; Wu, Qingwen

2018-03-01

In this paper, a novel method is proposed, which can simulate satellite platform micro-vibration and test the impact of satellite micro-vibration on imaging quality of space optical remote sensor on ground. The method can generate micro-vibration of satellite platform in orbit from vibrational degrees of freedom, spectrum, magnitude, and coupling path. Experiment results show that the relative error of acceleration control is within 7%, in frequencies from 7Hz to 40Hz. Utilizing this method, the system level test about the micro-vibration impact on imaging quality of space optical remote sensor can be realized. This method will have an important applications in testing micro-vibration tolerance margin of optical remote sensor, verifying vibration isolation and suppression performance of optical remote sensor, exploring the principle of micro-vibration impact on imaging quality of optical remote sensor.

Fabrication of micro/nano optical fiber by mechano-electrospinning

NASA Astrophysics Data System (ADS)

Chen, Qinnan; Wu, Dezhi; Yu, Zhe; Mei, Xuecui; Fang, Ke; Sun, Daoheng

2017-10-01

We study a novel fabrication method of micro/nano optical fiber by mechano-electrospinning (MES) direct-written technology. MES process is able to precisely manipulate the position and diameter of the electro-spun micro/nano fiber by adjusting the mechanical drawing force, which through changing the speed of motion stage (substrate). By adjusting the substrate speed, the nozzle-to-substrate distance and the applied voltage, the poly(methyl methacrylate) (PMMA) micro/nano optical fibers (MNOF) with controlled diameter are obtained and the tapered MNOF are fabricated by continuously changing the substrate speed. The transmission characteristics of PMMA micro/nano fiber is experimentally demonstrated, and a PMMA micro/nano fiber based refractive index sensor is designed. Our works shows the new fabrication method of MNOF by MES has the potential in the field of light mode conversion, optical waveguide coupling, refractive index detection and new micro/nano optical fiber components.

Pigments which reflect infrared radiation from fire

DOEpatents

Berdahl, P.H.

1998-09-22

Conventional paints transmit or absorb most of the intense infrared (IR) radiation emitted by fire, causing them to contribute to the spread of fire. The present invention comprises a fire retardant paint additive that reflects the thermal IR radiation emitted by fire in the 1 to 20 micrometer ({micro}m) wavelength range. The important spectral ranges for fire control are typically about 1 to about 8 {micro}m or, for cool smoky fires, about 2 {micro}m to about 16 {micro}m. The improved inventive coatings reflect adverse electromagnetic energy and slow the spread of fire. Specific IR reflective pigments include titanium dioxide (rutile) and red iron oxide pigments with diameters of about 1 {micro}m to about 2 {micro}m and thin leafing aluminum flake pigments. 4 figs.

Module Embedded Micro-inverter Smart Grid Ready Residential Solar Electric System

DOE Office of Scientific and Technical Information (OSTI.GOV)

Agamy, Mohammed

The “Module Embedded Micro-inverter Smart Grid Ready Residential Solar Electric System” program is focused on developing innovative concepts for residential photovoltaic (PV) systems with the following objectives: to create an Innovative micro-inverter topology that reduces the cost from the best in class micro-inverter and provides high efficiency (>96% CEC - California Energy Commission), and 25+ year warranty, as well as reactive power support; integrate micro-inverter and PV module to reduce system price by at least $0.25/W through a) accentuating dual use of the module metal frame as a large area heat spreader reducing operating temperature, and b) eliminating redundant wiringmore » and connectors; and create micro-inverter controller handles smart grid and safety functions to simplify implementation and reduce cost.« less

The role of microRNAs in synaptic development and function

PubMed Central

Corbin, Rachel; Olsson-Carter, Katherine; Slack, Frank

2015-01-01

MicroRNAs control gene expression by inhibiting translation or promoting degradation of their target mRNAs. Since the discovery of the first microRNAs, lin-4 and let-7, in C. elegans, hundreds of microRNAs have been identified as key regulators of cell fate determination, lifespan, and cancer in species ranging from plants to humans. However, while microRNAs have been shown to be particularly abundant in the brain, their role in the development and activity of the nervous system is still largely unknown. In this review, we describe recent advances in our understanding of microRNA function at synapses, the specialized structures required for communication between neurons and their targets. We also propose how these advances might inform the molecular model of memory. PMID:19335998

Estimate of the effect of micro-vibration on the performance of the Algerian satellite (Alsat-1B) imager

NASA Astrophysics Data System (ADS)

Serief, Chahira

2017-11-01

Alsat-1B, launched into a 670 km sun-synchronous orbit on board the PSLV launch vehicle from the Sriharikota launch site in India on 26 September 2016, is a medium resolution Earth Observation satellite with a mass of 100 kg. Alsat-1B will be used for agricultural and resource monitoring, disaster management, land use mapping and urban planning. It is based on the SSTL-100 platform, and flies a 24 m multispectral imager and a 12 m panchromatic imager delivering images with a swath width of 140 km. One of the main factors affecting the performance of satellite-borne optical imaging systems is micro-vibration. Micro-vibration is a low level mechanical disturbance inevitably generated from moving parts on a satellite and exceptionally difficult to be controlled by the attitude and orbital control system (AOCS) of a spacecraft. Micro-vibration usually causes problems for optical imaging systems onboard Earth Observation satellites. The major effect of micro-vibration is the excitation of the support structures for the optical elements during imaging operations which can result in severe degradation of image quality by smearing and distortion. Quantitative characterization of image degradation caused by micro-vibration is thus quite useful and important as part of system level analysis which can help preventing micro-vibration influence by proper design and restoring the degraded image. The aim of this work is to provide quantitative estimates of the effect of micro-vibration on the performance of Alsat-1B imager, which may be experienced operationally, in terms of the modulation transfer function (MTF) and based on ground micro-vibration tests results.

Hormonal regulation of Drosophila microRNA let-7 and miR-125 that target innate immunity.

PubMed

Garbuzov, Alina; Tatar, Marc

2010-01-01

The steroid 20-hydroxy-ecdysone (20-HE) and the sesquiterpenoid Juvenile Hormone (JH) coordinate insect life stage transitions. 20-HE exerts these effects by the sequential induction of response genes. In the nematode Caenorhabditis elegans hormones also play a role in such transitions, but notably, microRNA such as let-7 and lin-4 have likewise been found to help order developmental steps. Little is known about the corresponding function of homologous microRNA in Drosophila melanogaster, and the way microRNA might be regulated by 20-HE in the fly is ambiguous. Here we used Drosophila S2 cells to analyze the effects of 20-HE on D. melanogaster microRNA let-7 and miR-125, the homolog of lin-4. The induction by 20-HE of let-7 and miR-125 in S2 cells is inhibited by RNAi knockdown of the ecdysone receptor and, as previously shown, by knockdown of its cofactor broad-complex C. To help resolve the currently ambiguous role of 20-HE in the control of microRNA, we show that nanomolar concentrations of 20-HE primes cells to subsequently express microRNA when exposed to micromolar levels of 20-HE. We then explore the role microRNA plays in the established relationship between 20-HE and the induction of innate immunity. We show that the 3'UTR of the antimicrobial peptide diptericin has a let-7 binding site and that let-7 represses translation from this site. We conclude that 20-HE facilitates the initial expression of innate immunity while it simultaneously induces negative regulation via microRNA control of antimicrobial peptide translation.

Micro-Ramp Flow Control for Oblique Shock Interactions: Comparisons of Computational and Experimental Data

NASA Technical Reports Server (NTRS)

Hirt, Stephanie M.; Reich, David B.; O'Connor, Michael B.

2012-01-01

Computational fluid dynamics was used to study the effectiveness of micro-ramp vortex generators to control oblique shock boundary layer interactions. Simulations were based on experiments previously conducted in the 15- by 15-cm supersonic wind tunnel at the NASA Glenn Research Center. Four micro-ramp geometries were tested at Mach 2.0 varying the height, chord length, and spanwise spacing between micro-ramps. The overall flow field was examined. Additionally, key parameters such as boundary-layer displacement thickness, momentum thickness and incompressible shape factor were also examined. The computational results predicted the effects of the microramps well, including the trends for the impact that the devices had on the shock boundary layer interaction. However, computing the shock boundary layer interaction itself proved to be problematic since the calculations predicted more pronounced adverse effects on the boundary layer due to the shock than were seen in the experiment.

Ultra-short pulse delivery at high average power with low-loss hollow core fibers coupled to TRUMPF's TruMicro laser platforms for industrial applications

NASA Astrophysics Data System (ADS)

Baumbach, S.; Pricking, S.; Overbuschmann, J.; Nutsch, S.; Kleinbauer, J.; Gebs, R.; Tan, C.; Scelle, R.; Kahmann, M.; Budnicki, A.; Sutter, D. H.; Killi, A.

2017-02-01

Multi-megawatt ultrafast laser systems at micrometer wavelength are commonly used for material processing applications, including ablation, cutting and drilling of various materials or cleaving of display glass with excellent quality. There is a need for flexible and efficient beam guidance, avoiding free space propagation of light between the laser head and the processing unit. Solid core step index fibers are only feasible for delivering laser pulses with peak powers in the kW-regime due to the optical damage threshold in bulk silica. In contrast, hollow core fibers are capable of guiding ultra-short laser pulses with orders of magnitude higher peak powers. This is possible since a micro-structured cladding confines the light within the hollow core and therefore minimizes the spatial overlap between silica and the electro-magnetic field. We report on recent results of single-mode ultra-short pulse delivery over several meters in a lowloss hollow core fiber packaged with industrial connectors. TRUMPF's ultrafast TruMicro laser platforms equipped with advanced temperature control and precisely engineered opto-mechanical components provide excellent position and pointing stability. They are thus perfectly suited for passive coupling of ultra-short laser pulses into hollow core fibers. Neither active beam launching components nor beam trackers are necessary for a reliable beam delivery in a space and cost saving packaging. Long term tests with weeks of stable operation, excellent beam quality and an overall transmission efficiency of above 85 percent even at high average power confirm the reliability for industrial applications.

[Pollution prevention and control of aqueous extract of astragali radix processed with ZrO2 inorganic ceramic membrane micro-filtration].

PubMed

Pan, Lin-Men; Huang, Min-Yan; Guo, Li-Wei

2012-11-01

To study the measures for preventing and controlling the pollution of aqueous extract of Astragali Radix proceeded with inorganic ceramic membrane micro-filtration, in order to find effective measures for preventing and controlling the membrane pollution. The resistance distribution, polymer removal and changes in physical and chemical parameters of the zirconium oxide film of different pore diameters were determined to analyze the state or location of pollutants as well as the regularity of formation. Meanwhile, recoil and ultrasonic physical measures were adopted to strengthen the membrane process, in order to explore the methods for preventing and controlling the membrane pollution. When 0.2 microm of ZrO2 micro-filtrated aqueous extract of Astragali Radix, the rate of pollution was as high as 44.9%. The hole blocking resistance and the concentration polarization resistance were the main filtration resistances, while the surface deposit resistance decreased with the increase in the membrane's hold diameter; after micro-filtration, the liquid turbidity significantly reduced, with slight changes in both pH and viscosity. The 0.2 microm ZrO2 micro-filtration membrane performed better than the 0.05 microm pore size membrane in terms of conductivity. The 0. 2 microm and 0.05 microm pore diameter membranes showed better performance in the removal of pectin. The ultrasonic measure to strengthen membranes is more suitable to this system, with a flux rate up by 41.7%. The membrane optimization process adopts appropriate measures for preventing and controlling the membrane pollution, in order to reduce the membrane pollution, recover membrane performance and increase filtration efficiency.

Selective inhibition of alpha1B-adrenergic receptor expression and function using a phosphorothioate antisense oligodeoxynucleotide.

PubMed

Gonzalez-Cabrera, P J; Iversen, P L; Liu, M F; Scofield, M A; Jeffries, W B

1998-06-01

To investigate alpha1B-adrenoceptor function, we developed a phosphorothioate antisense oligodeoxynucleotide (AO) to inhibit the expression of the alpha1B-adrenoceptor subtype in DDT1 MF2 cells. We measured the cellular uptake of the AO and its effect on alpha1B-adrenoceptor mRNA expression, protein density, and coupling to phospholipase C. Cells treated with either a control oligodeoxynucleotide (CO) or medium alone served as control groups. Confocal microscopy demonstrated that DDT1 MF2 cells internalized carboxyfluorescein-labeled (FAM) AO within 30 min. Analysis of cellular lysates showed that approximately 50% of the intracellular FAM-AO was present as an intact 18-mer for up to 48 hr. Incubation of cells with AO for 48 hr decreased alpha1B-adrenoceptor density ([3H]prazosin Bmax) versus control groups by 12% (1 microM AO) and 72% (10 microM AO). In time course experiments, AO (10 microM) reduced alpha1B-adrenoceptor density by 28, 64, and 68% versus controls after 24, 48, and 72 hr of exposure, respectively. alpha1B-Adrenoceptor mRNA concentration (measured by RT-PCR) was reduced by 25% in cells treated for 48 hr with 10 microM AO versus controls. AO pretreatment (10 microM, 48 hr) reduced the maximum response to agonist-stimulated [3H]inositol phosphate accumulation. The maximal response of the full agonist norepinephrine was reduced by 30% after AO treatment, and by 73% for the partial agonist naphazoline. In contrast, AO did not affect histamine-stimulated total [3H]inositol phosphate accumulation. Thus, AO effectively reduced alpha1B-adrenoceptor subtype expression and function in vitro, suggesting a potential to selectively inhibit alpha1B-adrenoceptor function in vivo.

Morphologies and optical and electrical properties of InGaN/GaN micro-square array light-emitting diode chips.

PubMed

Han, Dan; Ma, Shufang; Jia, Zhigang; Liu, Peizhi; Jia, Wei; Shang, Lin; Zhai, Guangmei; Xu, Bingshe

2018-04-10

InGaN/GaN micro-square array light-emitting diode (LED) chips (micro-chips) have been prepared via the focused ion beam (FIB) etching technique, which can not only reduce ohmic contact degradation but also control the aspect ratio precisely in three-dimensional (3D) structure LED (3D-LED) device fabrication. The effects of FIB beam current and micro-square array depth on morphologies and optical and electrical properties of the micro-chips have been studied. Our results show that sidewall surface morphology and optical and electrical properties of the micro-chips degrade with increased beam current. After potassium hydroxide etching with different times, an optimal current-voltage and luminescence performance can be obtained. Combining the results of cathodoluminescence mappings and light output-current characteristics, the light extraction efficiency of the micro-chips is reduced as FIB etch depth increases. The mechanisms of micro-square depth on light extraction have been revealed by 3D finite difference time domain.

Recent advances in micro-vibration isolation

NASA Astrophysics Data System (ADS)

Liu, Chunchuan; Jing, Xingjian; Daley, Steve; Li, Fengming

2015-05-01

Micro-vibration caused by disturbance sources onboard spacecraft can severely degrade the working environment of sensitive payloads. Some notable vibration control methods have been developed particularly for the suppression or isolation of micro-vibration over recent decades. Usually, passive isolation techniques are deployed in aerospace engineering. Active isolators, however, are often proposed to deal with the low frequency vibration that is common in spacecraft. Active/passive hybrid isolation has also been effectively used in some spacecraft structures for a number of years. In semi-active isolation systems, the inherent structural performance can be adjusted to deal with variation in the aerospace environment. This latter approach is potentially one of the most practical isolation techniques for micro-vibration isolation tasks. Some emerging advanced vibration isolation methods that exploit the benefits of nonlinearity have also been reported in the literature. This represents an interesting and highly promising approach for solving some challenging problems in the area. This paper serves as a state-of-the-art review of the vibration isolation theory and/or methods which were developed, mainly over the last decade, specifically for or potentially could be used for, micro-vibration control.

Development of the dynamic motion simulator of 3D micro-gravity with a combined passive/active suspension system

NASA Technical Reports Server (NTRS)

Yoshida, Kazuya; Hirose, Shigeo; Ogawa, Tadashi

1994-01-01

The establishment of those in-orbit operations like 'Rendez-Vous/Docking' and 'Manipulator Berthing' with the assistance of robotics or autonomous control technology, is essential for the near future space programs. In order to study the control methods, develop the flight models, and verify how the system works, we need a tool or a testbed which enables us to simulate mechanically the micro-gravity environment. There have been many attempts to develop the micro-gravity testbeds, but once the simulation goes into the docking and berthing operation that involves mechanical contacts among multi bodies, the requirement becomes critical. A group at the Tokyo Institute of Technology has proposed a method that can simulate the 3D micro-gravity producing a smooth response to the impact phenomena with relatively simple apparatus. Recently the group carried out basic experiments successfully using a prototype hardware model of the testbed. This paper will present our idea of the 3D micro-gravity simulator and report the results of our initial experiments.

Hormonal activation of let-7-C microRNAs via EcR is required for adult Drosophila melanogaster morphology and function

PubMed Central

Chawla, Geetanjali; Sokol, Nicholas S.

2012-01-01

Steroid hormones and their nuclear receptors drive developmental transitions in diverse organisms, including mammals. In this study, we show that the Drosophila steroid hormone 20-hydroxyecdysone (20E) and its nuclear receptor directly activate transcription of the evolutionarily conserved let-7-complex (let-7-C) locus, which encodes the co-transcribed microRNAs miR-100, let-7 and miR-125. These small RNAs post-transcriptionally regulate the expression of target genes, and are required for the remodeling of the Drosophila neuromusculature during the larval-to-adult transition. Deletion of three 20E responsive elements located in the let-7-C locus results in reduced levels of let-7-C microRNAs, leading to neuromuscular and behavioral defects in adults. Given the evolutionary conservation of let-7-C microRNA sequences and temporal expression profiles, these findings indicate that steroid hormone-coupled control of let-7-C microRNAs is part of an ancestral pathway controlling the transition from larval-to-reproductive animal forms. PMID:22510985

Experimental investigation of the micro-ramp based shock wave and turbulent boundary layer interaction control

NASA Astrophysics Data System (ADS)

Bo, Wang; Weidong, Liu; Yuxin, Zhao; Xiaoqiang, Fan; Chao, Wang

2012-05-01

Using a nanoparticle-based planar laser-scattering technique and supersonic particle image velocimetry, we investigated the effects of micro-ramp control on incident shockwave and boundary-layer interaction (SWBLI) in a low-noise supersonic wind-tunnel with Mach number 2.7 and Reynolds number Rθ = 5845. High spatiotemporal resolution wake structures downstream of the micro-ramps were detected, while a complex evolution process containing a streamwise counter-rotating vortex pair and large-scale hairpin-like vortices with Strouhal number Stδ of about 0.5-0.65 was revealed. The large-scale structures could survive while passing through the SWBLI region. Reflected shockwaves are clearly seen to be distorted accompanied by high-frequency fluctuations. Micro-ramp applications have a distinct influence on flow patterns of the SWBLI field that vary depending on spanwise locations. Both the shock foot and separation line exhibit undulations corresponding with modifications of the velocity distribution of the incoming boundary layer. Moreover, by energizing parts of the boundary flow, the micro-ramp is able to dampen the separation.

In situ thermomechanical testing methods for micro/nano-scale materials.

PubMed

Kang, Wonmo; Merrill, Marriner; Wheeler, Jeffrey M

2017-02-23

The advance of micro/nanotechnology in energy-harvesting, micropower, electronic devices, and transducers for automobile and aerospace applications has led to the need for accurate thermomechanical characterization of micro/nano-scale materials to ensure their reliability and performance. This persistent need has driven various efforts to develop innovative experimental techniques that overcome the critical challenges associated with precise mechanical and thermal control of micro/nano-scale specimens during material characterization. Here we review recent progress in the development of thermomechanical testing methods from miniaturized versions of conventional macroscopic test systems to the current state of the art of in situ uniaxial testing capabilities in electron microscopes utilizing either indentation-based microcompression or integrated microsystems. We discuss the major advantages/disadvantages of these methods with respect to specimen size, range of temperature control, ease of experimentation and resolution of the measurements. We also identify key challenges in each method. Finally, we summarize some of the important discoveries that have been made using in situ thermomechanical testing and the exciting research opportunities still to come in micro/nano-scale materials.

Evaluation of data quality, timeliness and acceptability of the tuberculosis surveillance system in Brazil's micro-regions.

PubMed

Silva, Gabriela Drummond Marques da; Bartholomay, Patrícia; Cruz, Oswaldo Gonçalves; Garcia, Leila Posenato

2017-10-01

This study aimed to evaluate quality, acceptability and timeliness of the data in the tuberculosis surveillance system in Brazilian micro-regions. An ecological cross-sectional study was carried out, after a qualitative stage for selecting indicators. All 558 Brazilian micro-regions were used as units of analysis. Data available in the National Notifiable Diseases Information System (SINAN), from 2012 to 2014, were used to calculate 14 indicators relating to four attributes: completeness, consistency, timeliness and acceptability. The study made use of cluster analysis to group micro-regions according to acceptability and timeliness. Three clusters were identified among the 473 micro-regions with optimal or regular completeness (70% to 100%) and with over five notifications. Cluster 1 (n = 109) presented mean timeliness of notification and treatment equal to 62.8% and 24.9%, respectively. Cluster 2 (n = 143) had a mean percentage of cases tested for HIV equal to 55.9%. Cluster 3 (n = 221) had the best performing tuberculosis indicators. Results suggest priority areas for improving surveillance of tuberculosis, predominantly in the central-north part of the country. They also point to the need to increase the timeliness of treatment and the percentage of cases tested for HIV.

In-vitro trials to ascertain sustained release efficacy of assembly pheromone micro particles for the control of brown dog tick, Rhipicephalus sanguineus.

PubMed

Bhoopathy, Dhivya; Bhaskaran Ravi, Latha

2017-12-01

Sustained release micro particles were prepared incorporating assembly pheromone and deltamethrin. Two natural polymers, namely, chitosan and calcium alginate and a synthetic polymer, poly-ε-caprolactone were used for encapsulating the assembly pheromone-acaricide combination. The micro particles were subjected to in vitro evaluation freshly after preparation and then at monthly intervals to assess their sustained release efficacy. The response of the unfed stages of dog tick, Rhipicephalus sanguineus to fresh and aged micro particles was assessed and results were recorded. The micro particles were found to release assembly pheromone in a sustained manner up to 2 months of study period.

In-Silico Identification Of Micro-Loops In Myelodysplastic Syndromes

NASA Astrophysics Data System (ADS)

Beck, Dominik; Brandl, Miriam; Pham, Tuan D.; Chang, Chung-Che; Zhou, Xiaobo

2011-06-01

Micro-loops are regulatory network motifs that leverage transcriptional and posttranscriptional control to effectively regulate the transcriptome. In this paper a regulatory network for Myelodysplastic Syndromes (MDSs) was constructed from the literature and publicly available data sources. The network was filtered using data from deep-sequencing of small RNAs, exon and microarrays. Motif discovery showed that micro-loops might exist in MDS. We further used the identified micro-loops and performed basic network analysis to identify the known disease gene RUNX1/AML, as well as miRNA family hsa-mir-181. This suggested that the concept of micro-loops can be applied to enhance disease gene identification and biomarker discovery.

Modification of adenylate cyclase by photoaffinity analogs of forskolin

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ho, L.T.; Nie, Z.M.; Mende, T.J.

1989-01-01

Photoaffinity labeling analogs of the adenylate cyclase activator forskolin (PF) have been synthesized, purified and tested for their effect on preparations of membrane-bound, Lubrol solubilized and forskolin affinity-purified adenylate cyclase (AC). All analogs of forskolin significantly activated AC. However, in the presence of 0.1 to 0.3 microM forskolin, the less active forskolin photoaffinity probes at 100 microM caused inhibition. This inhibition was dose-dependent for PF, suggesting that PF may complete with F for the same binding site(s). After cross-linking (125I)PF-M to either membrane or Lubrol-solubilized AC preparations by photolysis, a radiolabeled 100-110 kDa protein band was observed after autoradiography followingmore » SDS-PAGE. F at 100 microM blocked the photoradiolabeling of this protein. Radioiodination of forskolin-affinity purified AC showed several protein bands on autoradiogram, however, only one band (Mr = 100-110 kDa) was specifically labeled by (125I)PF-M following photolysis. The photoaffinity-labeled protein of 100-110 kDa of AC preparation of rat adipocyte may be the catalytic unit of adenylate cyclase of rat adipocyte itself as supported by the facts that (a) no other AC-regulatory proteins are known to be of this size, (b) the catalytic unit of bovine brain enzyme is in the same range and (c) this PF specifically stimulates AC activity when assayed alone, and weekly inhibits forskolin-activation of cyclase. These studies indicate that radiolabeled PF probes may be useful for photolabeling and detecting the catalytic unit of adenylate cyclase.« less

Optimal design and experimental analyses of a new micro-vibration control payload-platform

NASA Astrophysics Data System (ADS)

Sun, Xiaoqing; Yang, Bintang; Zhao, Long; Sun, Xiaofen

2016-07-01

This paper presents a new payload-platform, for precision devices, which possesses the capability of isolating the complex space micro-vibration in low frequency range below 5 Hz. The novel payload-platform equipped with smart material actuators is investigated and designed through optimization strategy based on the minimum energy loss rate, for the aim of achieving high drive efficiency and reducing the effect of the magnetic circuit nonlinearity. Then, the dynamic model of the driving element is established by using the Lagrange method and the performance of the designed payload-platform is further discussed through the combination of the controlled auto regressive moving average (CARMA) model with modified generalized prediction control (MGPC) algorithm. Finally, an experimental prototype is developed and tested. The experimental results demonstrate that the payload-platform has an impressive potential of micro-vibration isolation.

Kit formulated asialoglycoprotein receptor targeting tracer based on copolymer for liver SPECT imaging.

PubMed

Liu, Chang; Guo, Zhide; Zhang, Pu; Song, Manli; Zhao, Zuoquan; Wu, Xiaowei; Zhang, Xianzhong

2014-08-01

Specific targeting of galactose-carrying molecule to ASGP-R in normal hepatocytes has been demonstrated before. In this study, galactosyl polystyrene was synthesized from controllable ratio of functional monomers and radio-labelled with (99m)Tc by formulated kit for SPECT imaging of hepatic function. p(VLA-co-VNI)(46:54) was synthesized by free-radical copolymerization initiated by AIBN, purified by dialysis, lyophilized to kit with Tricine and TPPTS as co-ligands for (99m)Tc labeling. Radiotracer (99m)Tc-p(VLA-co-VNI)(46:54)(Tricine)(TPPTS) was prepared and evaluated by in vitro stability, in vivo metabolism, ex vivo biodistribution and microSPECT/CT imaging in normal KM mice. MicroSPECT/CT and microMRI imaging were also performed in C57BL/b6 mice with xenograft hepatic carcinoma for hepatic function evaluation. (99m)Tc-p(VLA-co-VNI)(46:54)(Tricine)(TPPTS) was obtained in high radio chemical purity (RCP) (>99%) by using instant kit without further purification and excellent in vitro and in vivo stability. The result of biodistribution showed that liver had high uptake (90.49±10.68 ID%/g) at 30 min after injection and was blocked significantly by cold copolymer. MicroSPECT imaging in normal KM mice at 1h and 4h after injection showed good liver retention and targeting properties. Significant defect of activity was observed in the tumor site which was confirmed by MRI imaging. (99m)Tc-p(VLA-co-VNI)(46:54)(Tricine)(TPPTS) with lower ratio of targeting moiety has no observable effect on the specific binding affinity and liver uptake. This makes it possible to introduce more imaging units for multi-modality imaging. Furthermore, the instant kit preparation of (99m)Tc-labeling provides great potential for the evaluation of hepatocyte function in clinical application. Copyright © 2014 Elsevier Inc. All rights reserved.

Development and analysis of new type microresonator with electro-optic feedback

NASA Astrophysics Data System (ADS)

Janusas, Giedrius; Palevicius, Arvydas; Cekas, Elingas; Brunius, Alfredas; Bauce, Jokubas

2016-04-01

Micro-resonators are fundamental components integrated in a hosts of MEMS applications: safety and stability systems, biometric sensors, switches, mechanical filters, micro-mirror devices, material characterization, gyroscopes, etc. A constituent part of the micro-resonator is a diffractive optical element (DOE). Different methods and materials are used to produce diffraction gratings for DOEs. Two-dimensional or three-dimensional periodic structures of micrometer-scale period are widely used in microsystems or their components. They can be used as elements for micro-scale synthesis, processing, and analysis of chemical and biological samples. On the other hand micro-resonator was designed using composite piezoelectric material. In case when microscopes, vibrometers or other direct measurement methods are destructive and hardly can be employed for in-situ analysis, indirect measurement of electrical signal generated by composite piezoelectric layer allows to measure natural frequency changes. Also piezoelectric layer allows to create a novel micro-resonator with controllable parameters, which could assure much higher functionality of micro-electromechanical systems. The novel micro-resonator for pollution detection is proposed. Mathematical model of the micro-resonator and its dynamical, electrical and optical characteristics are presented.

Mechanical and Thermal Properties of Dental Composites Cured with CAD/CAM Assisted Solid-State Laser

PubMed Central

De Santis, Roberto; Gloria, Antonio; Maietta, Saverio; Martorelli, Massimo; De Luca, Alessandro; Spagnuolo, Gianrico; Riccitiello, Francesco; Rengo, Sandro

2018-01-01

Over the last three decades, it has been frequently reported that the properties of dental restorative composites cured with argon laser are similar or superior to those achieved with conventional halogen and light emitting diode (LED) curing units. Whereas laser curing is not dependent on the distance between the curing unit and the material, such distance represents a drawback for conventional curing units. However, a widespread clinical application of this kind of laser remains difficult due to cost, heavy weight, and bulky size. Recently, with regard to the radiation in the blue region of the spectrum, powerful solid-state lasers have been commercialized. In the current research, CAD (computer-aided design)/CAM (computer-aided manufacturing) assisted solid-state lasers were employed for curing of different dental restorative composites consisting of micro- and nanoparticle-reinforced materials based on acrylic resins. Commercial LED curing units were used as a control. Temperature rise during the photopolymerisation process and bending properties were measured. By providing similar light energy dose, no significant difference in temperature rise was observed when the two light sources provided similar intensity. In addition, after 7 days since curing, bending properties of composites cured with laser and LED were similar. The results suggested that this kind of laser would be suitable for curing dental composites, and the curing process does not suffer from the tip-to-tooth distance. PMID:29584683

MicroRNAs expression profile in solid and unicystic ameloblastomas.

PubMed

Setién-Olarra, A; Marichalar-Mendia, X; Bediaga, N G; Aguirre-Echebarria, P; Aguirre-Urizar, J M; Mosqueda-Taylor, A

2017-01-01

Odontogenic tumors (OT) represent a specific pathological category that includes some lesions with unpredictable biological behavior. Although most of these lesions are benign, some, such as the ameloblastoma, exhibit local aggressiveness and high recurrence rates. The most common types of ameloblastoma are the solid/multicystic (SA) and the unicystic ameloblastoma (UA); the latter considered a much less aggressive entity as compared to the SA. The microRNA system regulates the expression of many human genes while its deregulation has been associated with neoplastic development. The aim of the current study was to determine the expression profiles of microRNAs present in the two most common types of ameloblastomas. MicroRNA expression profiles were assessed using TaqMan® Low Density Arrays (TLDAs) in 24 samples (8 SA, 8 UA and 8 control samples). The findings were validated using quantitative RTqPCR in an independent cohort of 19 SA, 8 UA and 19 dentigerous cysts as controls. We identified 40 microRNAs differentially regulated in ameloblastomas, which are related to neoplastic development and differentiation, and with the osteogenic process. Further validation of the top ranked microRNAs revealed significant differences in the expression of 6 of them in relation to UA, 7 in relation to SA and 1 (miR-489) that was related to both types. We identified a new microRNA signature for the ameloblastoma and for its main types, which may be useful to better understand the etiopathogenesis of this neoplasm. In addition, we identified a microRNA (miR-489) that is suggestive of differentiating among solid from unicystic ameloblastoma.